LOCUS       DAORHD010000001        18587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_2097_length_18587_cov_3.982625, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000001 DAORHD010000000
VERSION     DAORHD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 18587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18587
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2097_length_18587_cov_3.982625"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="PKO06_00005"
     CDS             complement(<1..1342)
                     /locus_tag="PKO06_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68045.1"
                     /translation="MNRRPSRGAALQMILFLVLLISGLALAFFQMSSQAQRSAFRLER
                     GEVLRQIVEAADEEAFAVLLSALERPDSPEFLWLLNQGKNPAVLPISVPLISQIATTM
                     VRARQAAAITVEARVVDFTHRDFEGMPYSTDTREGVGRIELKTVCRLSDLGGGGGNAA
                     CHLIRRRDYKMATIVSPRPNTGRTGASQNAALDYALLVRNGLREFQQTGGLMLNQNRA
                     RLIVDAAGVPPGRRGKVRFGGADGRGDAHVFLNVDESRKAMMPAAPSKVTITISECHT
                     LFDLDTWKQQEIAAKVQKAKDKLGSQYDSSVEDYIRADVLKKFSGIVGEFKTGVAPLA
                     GTGPTEAADRVRQHLWNEEWKNLAGRVTSIPDRYDAPAPGCAMLSADAVGVTADVAQE
                     VLEGGIRQRFLYHAALHLDISSLEDQQQADEIGRKAWLGLNPAPSGLTAAADQHF"
     gene            complement(1375..3726)
                     /locus_tag="PKO06_00010"
     CDS             complement(1375..3726)
                     /locus_tag="PKO06_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68046.1"
                     /translation="MSDQETVQYLRDLRAPIKSFRLYAIEYFIREGRNREVVSALETA
                     QKQENDEECRLLLTHALNAVKQRAIEALEPASKVEKPLDAAGFPVRYSVADAEERLAL
                     LRRVKNDQLLVLAPFAVDAFQQEKHPLVAREIIRRFHPVWPKDRRQVLGRSLASEWQT
                     VRFAALEALTIIAPRDLIVHLPALLRHADPRVRSMAIRALAAIDPSEAAKHLEALLLS
                     PDVHHRWSALRECARLPFELTKPLLLAFLAVENDAALLDAACAVVAANPDPEVPWRVA
                     DLLAQRPEHAQILEPLLQTSINALQVSGQLSEKVEVYRQRLQQQVEQATVLRQARQAL
                     AALQTSDGTLQAEAQAVLRRCLPYPRVREMIADEVWKSTSESLRARLQEVLKSPSEPT
                     AEKDTDTWERCQSDAEKVRWLATRQAGQVGQLTRLLPGILSAPSTSASIKAAALRTAC
                     RCRVPGLTPLIDSFLRHSDDSVVAAALEYHAQTSPDEANALIGRYLKSGRSRVRVAAI
                     RVLRRTDPLQAVSSLFAMLNQKDPGDRALSLQCLVQFEFSLVRDRLLDLLESGKAPEL
                     SEDILALFEANPEVGLLYPLFRLARRLGGAKANQVDRVRSRMAELLVKMGHLKPKTLA
                     ELEGTFPARLQEDRKRRPMPAPAYSLKVLTQESESFWTKLGHTAGQGVKIVAALLLVI
                     LVAGVLLSRWAGGAGDPKELKKRTGAVIQVEPARGSGRILQITDFGWKVRLENGAIWH
                     LSPPPGGFPFIIEGMKVAVEARLFRVGADGAFQGTCVSFKISL"
     gene            3880..4230
                     /locus_tag="PKO06_00015"
     CDS             3880..4230
                     /locus_tag="PKO06_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV68047.1"
                     /translation="MATSIHAVEQSGFPVYSIHGYFDEAIGRELNALADQQLIKGKVC
                     LIVDLTDCPVMNSMGVAQLLELTVRVVEDFAGHLLLVGVNSVVLKVIKLAGVVPQAEL
                     SQTLTQALTEAQKV"
     gene            complement(4280..4918)
                     /locus_tag="PKO06_00020"
     CDS             complement(4280..4918)
                     /locus_tag="PKO06_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68048.1"
                     /translation="MTNHIRLFHIFRNAFQNPMMRLTRLGLMVLLLGLSVGVYAETTG
                     EPVPTKILPASVAEPGSLDQVKAPVGDLTMFCWFQPPEASRFGVADKDGGIVWEKVAG
                     FRPMLDYFWSKDGSKIFYVTDCIQKEAELRAGSDKTRSWFFVLDAMSGKTLAEGDLDT
                     DILDLPKQLPDAVGASHFIEVAFENDVINVTIEHQGKKVSGSKALKDLLEKK"
     gene            complement(4930..5256)
                     /locus_tag="PKO06_00025"
     CDS             complement(4930..5256)
                     /locus_tag="PKO06_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68049.1"
                     /translation="MNITQYGGPSDRTPDKYTKMGLGNRNNKLRPTSLALSPDLIRKY
                     GLKGGESIYIKTSQGTRFLGHYDDTTGNKNEPNVIDIYDKTDSLGNDNFLATIPKGQW
                     ELVIGR"
     gene            5249..5806
                     /locus_tag="PKO06_00030"
     CDS             5249..5806
                     /locus_tag="PKO06_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68050.1"
                     /translation="MFKRPSNAFDPRLMNPLETIDCALAAALPEGLTAGALVVPAVEE
                     LEDEEEVEPPVVPVVVVPSSVFVGALVPVGTVPVGDWEVCCPLVCSPVCAACCPAELM
                     VLPTLLIFCPASGLKMLTIDPTIPVSEPIDDRIGARDDNQEHPVKPADIRTTKTMKSP
                     SLQNRLKVRMPIISASYLSSTRRSR"
     gene            complement(5918..7231)
                     /locus_tag="PKO06_00035"
     CDS             complement(5918..7231)
                     /locus_tag="PKO06_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNV68051.1"
                     /translation="MELTAELVTQGAYALGIGILVGLERSVFDQPVPDESTESEAIEE
                     KESSELEGGEPSGVRTFSTFSLVGFAAAAVNVHVPFAGPVILAGLVLLVLALYRHTLV
                     RSPGMTTEAAAIGTAVLGAMCHSNPHFAAVLAVVLTGILSSKQFMHETIRKMRRVEVT
                     DTLKFLVIILIIVPLLPNRPLDPWGAVNPYKIGWLVVLISGISFVGYFLTRIMGAQKG
                     LGLTGLLGGLTSSTAVTAAMAEESTAAPQLKAICAFATVVANATSFLRVLVMVVILDL
                     TLAKQLAWSIGGMALTAVVAAILLWMAASRDKVTEIKEREGQVKLKNPFALTPALKFA
                     AFFVLIIFVSKVAREKLGNAGIYLAAAFSGLADVDAITLSMAEQTGNGSLESSVAVLG
                     ITIAVVSNAVVKSGIAMFTGSRDFGRLVALSLGAAVIVGLLMACI"
     gene            7477..9867
                     /locus_tag="PKO06_00040"
     CDS             7477..9867
                     /locus_tag="PKO06_00040"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV68052.1"
                     /translation="MSFSRGSWVHFLIMLLLVACVWQLEQPPHAKPSDVSVQEFSAHR
                     ALQLIRGYAVQPHRAGTAANDVLRQKLIDALTDLRLIVVTEEPTICRGTRVGNPRNIY
                     ARLPGRRSQLASASFEAMASGTMAGAPASLSARVASFPSAFLLMAHYDSVPFGPGAAD
                     DLAGVAAILEAVRAIQAGPPLANDLIIAFTDGEECGLLGAKAFSQHPWAAEVGCLLNF
                     EARGICGPSYLFELGAPKTWLVAEMARSGAPLRTSSLMFDIYRRLPFSTDYHLLRDSI
                     PGMNAAFIGYFPYYHSSHDTSEHLSSRSLQHHGEYALAFARHFGSLDRLATHPRSDLI
                     YFSGFGSQVIQYSHEFGIFLTIILTACSVFVLRLPFRDPDGQRSAPDHTTPDNILVQS
                     APAHWRTAFKASFSLSLLAGLFSLIPLIGGYLVHGPYLLYANWWVILAGVSLALACFA
                     AKYAKYRLHISLTIIHKGFIGLWIIGMLVQQLFLPAGTFLWHWPLLFGLLAITISAIF
                     SRHRHFAFIDLGLFTLLTATLSGISVPLCFSLGEVIGVFSVPLLSVWLILIAGLLLPW
                     LDPTVVSRKRGLVLTFSLSSLAGFVGALALAAPSIDCPRMTHLLQDIDAHTSTTRFYS
                     AETHLSDWTKEQLDSDTLKVGMRHAPGHLLWQGSTRQVSETGTLAQLASETTGSGSQR
                     ILSIVLTPTNKAQRLLIQGSSATEVAEVAFGRTSVELHGKAWEVDYSILPRNPLQMTV
                     TLAASAALDLRLTEIRYGIPVSRSPRAPGLIPMNNVLNRGSRFESDTVHVRTHYRL"
     gene            complement(9900..12194)
                     /locus_tag="PKO06_00045"
     CDS             complement(9900..12194)
                     /locus_tag="PKO06_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68053.1"
                     /translation="MPTTSASTTRHQWRFYRCGGLDQVSIESGADLLALGQLDSKLWA
                     TLSCPTKGVELDEKTLHLVDSDQDGRIRVAEVLAAVAWAGERLRNPDDLLKRSAVLPL
                     SAVNEQHADGKRLLSAMRQILKTLGKEQATEITADDVRSMVAMFAQTRLNGDGIVPAT
                     AASKEDLKTLIEEMISCVGSENDRSGLPGVSSVLVDRFWAELHAHDEWYKLLEADSKR
                     VLPFGENTASAFGLWQELAPKVEDFFTRCRLLAFDERLAERVTGSQAEPPLPTMAMLS
                     ATGESVRALPLAHPSATAELPLTDRVNPAWADCLARFKREVVEPMLGSRTQLTHEDSQ
                     KLVECFAPYQSWLAKKPSSSVEKLGMKRIRELIAGDHEKRVRALIADDLALKAEFDSI
                     SEVEKLVLYHRDLATLINNFVTFLDFFSGKHQAMFQAGTLYIDGRACELCLRVEDAGR
                     HASLSALCNTYLAYCDCVRRGMSEKMTIVAAFTAGDAEQLSVGRNGVFFDRRGNEWDA
                     TIVKIVEHPISIGEAFWSPYRRLGRMISEQVEKFAASGDKEMQARMSSGVAEAGKTVT
                     AGAPAPAQPFDVGRFAGIFAAIGLAIGAIGTAVASVITGFLGLSWWQMPLAIVGLLLL
                     ISGPSMVLAWLRLGKRNLGPLLDANGWAVNTRATMNVRFAQSLTKVATLPDGAKRYLE
                     DPFADKTRPWLTYFLILALILGGGFALKKRGAIKKWLAHRGNPPAKSSIASGTPSVAT
                     GTVNPASPAAPSVVGTGSAPVSAP"
     gene            12490..13866
                     /locus_tag="PKO06_00050"
     CDS             12490..13866
                     /locus_tag="PKO06_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV68054.1"
                     /translation="MHLHSRISVIALLGFIACLCTPALPVCAGVFGQVERDSNTPPPE
                     NDTTTEGRPASDQSEPTRLMMRGASSMPAKGTLADRQEYRYVVTLPPAMDLSIELESQ
                     PANAAVPELLDRDGTKVELRDEGEGTWMGAVPATPDGKEADFTVVVHKKPTAATEVSF
                     ILSVRVPELGDEGGNSESMQQEPSISAGDSSQGKSDRRTVIAKSGLSLRARPDPKATK
                     LATLPFGTVVEVLDIDGPPDMYEGINAQWYKIRANGKEGWAFGGFIGEPGEMPTPEGS
                     GMRGAIFNQPWEERKDGMRFFELSDPGTEKLKLQNGTWLTLEKGKTSGKFEFTNEGYL
                     FYDGVFISRNFVAEYVSSTDDPSGKMVPEMATKLIFSPASPSGRQIFFTATRDSGCVN
                     EYLLNLDEGVILNVSISKYGVDEWIEWSNDGKSVQLRYETEGSRFIYRLDIKTGKETL
                     LKEETIAE"
     gene            complement(13969..14481)
                     /locus_tag="PKO06_00055"
     CDS             complement(13969..14481)
                     /locus_tag="PKO06_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68055.1"
                     /translation="MNYSRSLCVLVLMAVLFVVPTAMAGVVDAPAGDPGQAGQSEQAG
                     QADQSGGQGSDCAILAWVKKLFHKDPALQKEAMLKLLELVVKDIELALNTLGGKDVCY
                     LKMFPAIEHVNHALKTLNKIHPPKHLKEVFKQVEKRLSHTKFCLVVKDTDEASARLQS
                     ALEYIKTLQQ"
     gene            complement(14622..15587)
                     /locus_tag="PKO06_00060"
     CDS             complement(14622..15587)
                     /locus_tag="PKO06_00060"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HNV68056.1"
                     /translation="MTSVLHHQPPEHPRFPKDFPEFERWFDTEAACERYLYEQRWPEG
                     FVCPACGHGQAWKNRRGWLECAGCHRQTSLTAGTLFHRSRLPLRTWFLAMWFICTSKT
                     GVSAAGLKRLLGLNSMQTAFSWLRRLRQAMVRPDREPLEGWVQVDDAWLAGSEAGKGS
                     SWRSALIAVAVESDASGDEFGRIRLRDITTEPAAGLVRFIRDEVKPGTQLEVDGWEGY
                     ETLAEEGYRCEIVAAGDDPSTPLPRVRRVVGLIRRWLLGTHQGRLSRAFLQGYLDEFA
                     FRYNRRKSPSVGMLFQRLIWHGARMQGQAYRPKSRDNMNRMEKLS"
     gene            complement(15720..16265)
                     /locus_tag="PKO06_00065"
     CDS             complement(15720..16265)
                     /locus_tag="PKO06_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68057.1"
                     /translation="MKMAKVEMMIWVVVLVGYLSQPLLAIMPTELNLGDLGVVKVVEG
                     TPPPETTGRFDKLVLMPPSGTGEGKVIFETEGRILEKWEIVSLAGTESRQLLLTLDPG
                     GNGGFKEFALLVPMNQGFEVIWEETMKMGSAEVKDVNGDQKPDLVLTGTEMDPATGKV
                     RPQTMEFTWTDGKLLALSQPE"
     gene            complement(16306..17469)
                     /locus_tag="PKO06_00070"
     CDS             complement(16306..17469)
                     /locus_tag="PKO06_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68058.1"
                     /translation="MSPVVRQVLMLLLLCGCVLTQNGYAETPVAEVKIERELFPQPYE
                     RLLDGYAYPEDRLLALFRCLCLARSDAHLSVAIISLERAIGELMRTQSALLRHQLLTA
                     EDPKQRMQEWMVWWSTVARRAETAAFASLFRLMSTEEKARYGFAALEKESLEQENELY
                     DPDYLAAYADVIPERHEFTRQIHKVYWCEDSVTDPLESLMTRLKFAFSTEEVLRYLAR
                     LCGRMSDSPLRQSLEKLQTRLTGNLVDDIIHENGVLLENNLYGSEIALIGVTAAGRVL
                     IRESLIRGRLQLLVKHAQGIRSRLDREIFVPILEQIGRRLVELGANSSESVKLHLQHM
                     RERLQEIVNATVTADAPADDQTDLVLFRKMGEYLVAEDRNSLMRGAPVLFARP"
     gene            17690..>18587
                     /locus_tag="PKO06_00075"
     CDS             17690..>18587
                     /locus_tag="PKO06_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68059.1"
                     /translation="MEHVLSLPTVSRRALLLFWLVVLCVPLLFLRSSMVSLIREDERN
                     LTEQARRALREEMTAFLDDLSLPKQILNSLRSVESRSSRLATLTGLKQLDSEQGIADL
                     LLLELARELPAEPLFLLIDPGHGRPPEIRYNTRLFTPLSAPSRRLAELWLRSLKDVLS
                     PEERQRLDRTVCNLLGELVSTNLPPDQVQESFSVRLGHERLLLAGRTLTETPTAPFRR
                     WIIAFRDRDFRLTQIIRQAVSQSLRHQCRRQVRFSTTDESGATFDLTRAHLRYSLPMP
                     RLLFAQGTDIPQRGIESPLPRPQ"
BASE COUNT         3989 a         5750 c         4984 g         3864 t
ORIGIN      
        1 aaaagtgttg atcagccgcc gccgtcagcc cactcggagc gggattcaac cccagccacg
       61 ctttcctgcc gatctcgtcc gcctgctgtt gatcttcgag tgaagagata tcgagatgca
      121 gagcggcatg atacagaaag cgttgccgaa tgcccccttc cagcacttcc tgggccacat
      181 cggcggttac tccgacggca tccgcgctca gcatcgcaca tcccggtgcc ggagcatcgt
      241 accgatcggg aatgctcgtc acgcgaccgg caagattctt ccactcttcg ttccacaggt
      301 gctgccgcac ccgatcagca gcctcggtag ggccggttcc tgccaggggt gccacaccgg
      361 tcttgaattc acccacgatg ccactgaact ttttcaggac atcggctcga atgtagtcct
      421 cgacactgct gtcatactgc gagcccagct tgtcttttgc cttctgtact ttggcggcga
      481 tctcctgctg tttccaggta tcgagatcaa acagggtgtg acactcggaa atcgtgatcg
      541 tcactttcga cggcgcagcc ggcatcatcg ctttgcgaga ctcgtcgacg ttcagaaaga
      601 catgggcatc accgcgaccg tctgcgcccc cgaaccgcac ctttccgcga cggcccggcg
      661 gaacacccgc ggcatccacg atcagacggg ctcgattctg gttcagcatc aagccacccg
      721 tctgttgaaa ctcccgcagg ccgttgcgta ccagaagagc atagtccagg gcggcattct
      781 gtgaagctcc cgtgcgacct gtgtttgggc gcggtgaaac gatggtcgcc atcttgtaat
      841 cacgccgacg aatcaggtga caggcggcgt tgccaccacc gcccccaaga tcggacaacc
      901 gacacacggt cttcagctcg atacgcccga cgccctcacg cgtatcggtt gagtagggca
      961 tgccttcaaa gtcacgatgc gtgaaatcga ccacgcgcgc ttcgacggta atcgctgccg
     1021 cctgccgggc tcgaaccatg gtggtcgcga tttgactgat caatggcacg gaaatcggca
     1081 gtaccgcggg gttctttccc tgattgagca accagagaaa ctcgggagaa tcgggacgct
     1141 ccagagcaga caacagcacc gcgaacgctt cttcatcggc ggcttcaacg atctgtcgca
     1201 gcacctcgcc gcgttcgagg cgaaaggcac ttcgctgcgc ctgtgatgac atttgaaaaa
     1261 atgccagcgc cagaccggaa atgagcagca cgagaaacag gatcatctgc agtgccgcac
     1321 cccgagatgg tcgacgattc atggatatcg cattatccca tttgttcaca gccactacaa
     1381 gctgattttg aacgagacgc aggttccctg aaacgccccg tctgctccga cgcgaaacag
     1441 acgcgcctcg accgcgacct tcattccttc gatgatgaaa gggaaccccc cgggaggtgg
     1501 cgacagatgc cagatcgcac cgttttcgag tcgtaccttc cagccgaaat ccgtgatctg
     1561 cagaatccga ccgctgccac gagcgggttc gacctgaatg acggctcccg ttcgtttctt
     1621 cagttctttc gggtcaccag caccacccgc ccagcgactc aacagcaccc cggccacaag
     1681 tatcacgagc agtaaagccg cgacgatctt caccccttgg ccggccgtgt ggccgagttt
     1741 tgtccagaac gactcggact cttgcgtgag cactttcagt gagtatgcgg gggccggcat
     1801 cggccgtcgc tttctgtctt cctgcagacg tgccgggaag gttccctcga gctctgccag
     1861 agtcttgggc ttcagatgcc ccattttgac cagcaactcc gccatccgcg accgcacccg
     1921 atcgacctga tttgctttcg cacctcccag gcggcgcgcc aggcgaaaca gcgggtagag
     1981 caatccgact tccgggttgg cttcgaacag ggccaggatg tcctcagaca gttccggagc
     2041 ttttccgctc tcaagcaaat cgagcaggcg gtcgcgcacc agcgaaaatt cgaattgtac
     2101 aagacactga agcgacagag cccgatcacc aggatctttc tgattcagca tagcaaacag
     2161 cgatgacacg gcctgcagcg ggtcggtacg ccgcagcacc cgaatggcgg ccacacgcac
     2221 ccgcgaacgc ccggacttga gataccgtcc gatcaaggcg ttggcttcgt cgggagaggt
     2281 ctgcgcgtgg tattccagtg ccgccgccac cacgctgtca tccgagtgcc gcaggaatga
     2341 atcgatcagc ggagtcagac cgggtacccg acaacgacag gcagtccgca acgccgcggc
     2401 tttaatcgat gccgaagtgg agggggcaga gagaataccg ggcaacagtc gggtgagttg
     2461 tcctacctgc ccagcctgtc gcgtcgcgag ccaacgcact ttctcggcat cagattgaca
     2521 gcgttcccag gtgtcggtgt ctttttccgc tgtgggttcc gatggcgatt tcaacacttc
     2581 ctgcagtcga gcacgaagtg attcagaggt cgacttccac acctcatcgg caatcatctc
     2641 ccgaacacgc gggtatggaa ggcaccgacg caagaccgcc tgcgcttcgg cttgcagtgt
     2701 tccgtcactc gtctgcaggg cagccagagc ctgacgcgcc tgacgcagca cggttgcctg
     2761 ctccacctgc tgctgtaatc gctgacgata gacttccacc ttctcagaca actgccccga
     2821 aacctgcagg gcattgatcg acgtctgcag cagcggttcc agaatctgag cgtgttcggg
     2881 acgctgcgcc agcaggtcgg cgacccgcca tggcacttcg ggatcggggt tggcagctac
     2941 cacggcgcag gccgcatcga gtaatgcagc atcgttttca accgccagaa acgcgagcaa
     3001 cagcggcttg gtcaactcaa agggaaggcg agcacattcc ctgagggcag accatcgatg
     3061 atgaacatca gggctcaaga gaagtgcttc cagatgtttc gcggcctcgg agggatcgat
     3121 ggcggccaat gcgcgaatcg ccatgctgcg cacccgagga tcggcatggc ggagcagcgc
     3181 gggcaaatga acgatcaagt cgcgtggagc aataatcgtg agcgcttcga gcgcggcaaa
     3241 gcgaaccgtc tgccactcag aagccagaga acgtcccagg acctgacggc gatctttcgg
     3301 ccagaccggg tgaaagcgcc gaatgatctc gcgggccacc agcggatgct tttcctgctg
     3361 gaacgcatcg accgcgaacg gcgccagcac caggagctgg tcgttcttca cacggcgcag
     3421 caatgccagt cgttcttctg catcagcaac ggaatagcgg acagggaatc ccgccgcgtc
     3481 caggggtttc tccaccttcg atgccggctc cagagcttcg atggcccgtt gcttgaccgc
     3541 gttcagcgca tgcgtcagca gtaaccgaca ctcctcgtcg ttctcttgct tttgcgccgt
     3601 ttcgagggca ctgacaactt cccggttgcg accttcccga atgaagtact cgatggcgta
     3661 caaacggaac gatttgatcg gcgctcgaag atcgcgcaga tactgaacgg tttcttggtc
     3721 agacatggtg agaaatcacg agtgatgagc ctgtcgcccg gcagatcgag tatatcacac
     3781 gctcaagtct cccctgagcc gaaccgctca ttcagatcac cggctctcat gctatactca
     3841 gcgggataat tctctcatct cggcgaagcg atatcattca tggcaacctc catacacgca
     3901 gttgagcaga gcggttttcc ggtgtactcg attcacggat actttgatga agcgatcggt
     3961 cgcgagctca atgcccttgc tgatcagcag ctgatcaaag ggaaggtctg cctgatcgtc
     4021 gacctcaccg actgtcccgt gatgaacagc atgggagtcg cccaacttct cgagctgacc
     4081 gttcgcgtcg tcgaggactt tgccggtcac ctgctgctgg tgggagtcaa ctccgtggtt
     4141 ttgaaggtga tcaaacttgc cggagtcgtg ccacaggctg agctgtctca gaccctgacc
     4201 caggctctca ctgaggctca gaaagtgtaa cgtcgactcg aacgccagca gcgctcgaat
     4261 cgacgtcaat aatcgtccgt tactttttct cgagcagatc tttcaacgct ttcgaaccac
     4321 tcactttctt gccctggtgc tcgatcgtga cattgatcac atcattttcg aacgcgacct
     4381 cgatgaagtg agaagcgccc accgcatccg gcaactgttt cggaagatcg agaatatcag
     4441 tatcgagatc gccttctgca agcgtctttc cgctcatggc atccaggaca aagaaccaac
     4501 tgcgggtttt gtccgagccc gctcgcaact cggcttcctt ctgaatacaa tcggtcacgt
     4561 agaagatctt cgaaccatct ttcgaccaga agtagtcgag catcgggcgg aaacccgcca
     4621 ccttttccca gacgatgccc ccgtctttgt ccgcgactcc gaaacgcgaa gcttcgggcg
     4681 gttgaaacca acagaacatg gtcaggtctc caaccggcgc tttcacctga tcgagtgatc
     4741 cgggctcggc cacgctcgca ggcaggatct tcgtcgggac gggttcaccg gtcgtttccg
     4801 catacactcc gactgacagg ccaagcaaca gaaccatcag tcccagtcgg gtcaagcgca
     4861 tcattgggtt ctgaaaggca ttacggaaga tatgaaacaa acgaatgtgg ttagtcatga
     4921 gtagcctcct tagcgaccga tgaccagttc ccattgtcct ttgggaatcg tggccaggaa
     4981 gttatcgttt ccgagactgt cggtcttgtc atagatgtcg atcacattcg gttcattctt
     5041 gttgccggtg gtgtcgtcat agtgaccgag aaatctcgtt ccttgagatg ttttgatgta
     5101 gatggactcg ccgcctttga ggccatactt tctgatcaga tcgggcgaca acgccagcga
     5161 ggtcggacgc agcttgttgt tccggtttcc cagacccatc ttcgtgtatt tatcaggagt
     5221 gcgatcgctc ggcccaccat actgcgtgat gttcaagcga ccgtcgaacg ctttcgatcc
     5281 gcgattgatg aacccgctcg aaaccatcga ttgcgcattg gcggccgcgc ttccggaagg
     5341 gctgaccgca ggagcgctgg tggtccctgc tgtcgaggaa ctcgaagatg aagaagaggt
     5401 tgagccgcca gtcgtcccgg tcgtggtcgt tccgtcttcc gtgttcgtcg gagcgcttgt
     5461 tccagtgggc acggttccgg tcggcgactg ggaagtctgt tgtccgctcg tctgttcgcc
     5521 ggtttgtgcc gcctgttgcc ccgcagaact gatggtgttg ccgacgctgc tgatcttctg
     5581 ccccgcttca gggctgaaga tgctgacgat cgacccaacc attcccgtaa gcgaaccgat
     5641 agacgacagg atcggtgcca gggacgacaa ccaggaacac cctgtcaaac ccgccgacat
     5701 cagaaccacg aagacgatga aaagcccatc tctgcagaat cgcctcaagg tgcgcatgcc
     5761 catcatttca gcctcctatc tttcctcgac acgtcgaagt cgatgacttc gatgtactgc
     5821 cacccatcat accgggagaa acgctgttcg cctatccctt ttgtacaaaa tcattcacag
     5881 cggtaacgtt gcgtcagctc tggctcgggg aagggcctca gatgcaagcc atcagcagtc
     5941 cgacaatcac cgcggcgcca agggacaaag ccaccaggcg gccaaagtca cgactgccag
     6001 tgaacatggc gattcctgat ttcacgactg cattcgagac cacggcaatc gtaatgccca
     6061 gaaccgccac ggaagactcg agcgagccgt tccctgtctg ctcagccatc gacagggtaa
     6121 tggcgtcaac gtcggccaga cccgaaaaag cggcagcaag atagattccg gcgttcccca
     6181 gtttctcgcg cgccactttc gagacaaaga tgatcagcac aaagaaggcc gcgaacttca
     6241 gcgccggagt caacgcgaac gggttcttca gcttgacctg cccctcacgc tccttgatct
     6301 cggtcacctt gtctcgtgaa gccgccatcc agagcaggat tgctgccacg acggccgtca
     6361 gcgccatgcc accaatcgac caggctagct gcttggcgag cgtcagatcg agaatgacca
     6421 ccatgaccag cacgcgcagg aaagaagtgg cattcgccac cacggtcgca aacgcgcaga
     6481 tcgccttcag ttgaggcgcg gcggtcgact cttcggccat agcggcggtc accgcggtcg
     6541 acgaggtcag acctccgagc agaccggtca accccagacc tttctgtgcg cccatgatgc
     6601 gggtcaggaa gtatcccacg aagctgattc ccgaaatcag tacgaccagc cagccgatct
     6661 tgtagggatt gaccgcgccc cacgggtcga gcggccgatt cggcagcagc ggcacaatga
     6721 tcagaatgat cacgaggaat ttgagcgtgt cggtcacctc gacccgtcgc atcttccgga
     6781 tcgtctcgtg catgaactgc ttggaactga gaatgcccgt caaaacgacc gcgagcaccg
     6841 cggcgaaatg cgggttgctg tgacacatgg ctccgagcac ggcggtgccg atggctgccg
     6901 cttcggtggt catacccggg ctgcgtacca gcgtatgccg gtacaaagcc agaaccagca
     6961 gaaccagccc ggctagaatc accgggccag cgaacggaac atggacattc acggcggcgg
     7021 cggcaaatcc aacgagggag aaggtcgaaa acgtgcgcac accgctcggt tcgccccctt
     7081 ctaactcaga cgattccttt tcttcgatcg cctcagactc cgtcgactca tcgggaaccg
     7141 gttggtcgaa gacactgcgt tcgaggccga ccagtatgcc aattcccagc gcatacgcgc
     7201 cctgtgtcac caattcagcc gtcagttcca tggaatcctc gcaagagaac ttcagtgctg
     7261 ggaaccactg ccccgcacat ttttgtcacc tgcgcatctt atgctgctgc cgaaatcgac
     7321 gtcaagcggg cttccgagca atcttgagga gacgctcatc agcttgcgca ctggcgggac
     7381 ttcgggagcg ctcaccacaa tccaggtcgg gcgacctttt gtccgacctc tcgctcacgc
     7441 aggaagatca acgcatctcc tgatatcatg aaccctatga gtttctcgcg cggcagctgg
     7501 gtgcattttc tgatcatgtt gctgctcgtc gcctgcgtct ggcagcttga gcagcctcca
     7561 catgccaaac caagcgatgt ctcagtgcag gagttttcgg ctcatcgagc tctgcagctg
     7621 attcgtgggt atgccgtgca gccgcatcgc gccggaaccg cagccaatga tgtcctgcgc
     7681 cagaagctga tcgacgccct gaccgatctg cgcctgatcg ttgttaccga agaaccgacc
     7741 atctgccggg gcactcgagt cggaaacccg cgcaacatct acgctcgcct gcccggcagg
     7801 cgatcacagc ttgcctcagc ttcgtttgag gcgatggcgt cgggaacaat ggcgggtgca
     7861 cccgcctcac tctcagctcg ggttgccagc tttccgtctg catttctgct catggctcat
     7921 tatgattctg taccgttcgg gccaggtgcg gctgatgatc tggccggggt cgcggccatt
     7981 ctcgaggccg tgcgggcgat ccaggccggg cctcccctgg caaacgatct gatcatcgcc
     8041 ttcaccgacg gagaagagtg cggccttctg ggcgcgaagg ccttcagtca gcatccgtgg
     8101 gccgcggagg tgggctgtct tctcaacttc gaagcacgtg gcatctgcgg accatcctac
     8161 cttttcgaac tcggagcccc caaaacctgg ctggtcgcgg aaatggctcg atcgggagcc
     8221 ccactgcgca cttcgtcact gatgttcgac atctaccgcc gcctgccgtt cagtacggat
     8281 taccatctgc tgcgcgactc gatccccgga atgaacgccg ccttcatcgg atactttccg
     8341 tattatcact cgtctcacga cacttcggaa cacctgagta gccgcagtct gcagcaccac
     8401 ggcgagtatg ctctggcctt cgctcgtcat ttcggctcac tcgataggct cgccacacat
     8461 ccgcggtccg atctgatcta cttttcgggt tttggctcgc aagtgataca gtattcccat
     8521 gagttcggaa tctttctcac gatcattctc accgcctgtt cggtgtttgt tcttcgactt
     8581 ccttttcgtg acccggatgg tcagagaagc gcgcctgacc atacaacccc cgacaacata
     8641 ctcgtacagt cagcccccgc gcactggaga acggctttca aagcctcttt ttctctgagc
     8701 ctgctcgccg gtctgttcag tctgattccg ctgatcgggg ggtatctggt tcatggtccg
     8761 tatctgctct atgccaactg gtgggtcatc ctggccgggg tgtctctggc attggcctgt
     8821 ttcgcggcaa agtatgcgaa gtacaggcta catatttctc taacaatcat tcataaaggt
     8881 ttcattggac tctggataat aggaatgctc gttcaacaac tttttctgcc cgcgggcact
     8941 tttctctggc actggccact gcttttcggc ctgctcgcca ttaccatctc tgccattttc
     9001 tctcgtcaca ggcattttgc cttcatcgac ctcggactgt tcactctact cacagccact
     9061 ctgtctggaa tcagcgttcc tttgtgtttc tcgctgggcg aggtgatcgg tgtgttttca
     9121 gtgcccctgc tcagcgtctg gctgattctc atcgccggtc ttctcctgcc ctggctcgat
     9181 ccaaccgttg tctcgcgaaa gcgcggactg gtgttaactt tttcgctcag ttctctcgcc
     9241 ggcttcgtcg gtgcactggc tcttgctgcc ccctctatcg actgccctcg gatgacacat
     9301 cttcttcagg atatcgacgc acacacgagc acaacacgct tctacagcgc tgaaacgcat
     9361 ttatcagact ggacaaaaga gcagttggac tcagacacgc tcaaggtcgg catgcgtcat
     9421 gcacccggcc acctgctgtg gcaaggctcg accaggcagg tttcagagac aggcactctc
     9481 gcgcagctgg cctcagaaac gacaggcagc ggttctcagc gcatcctgtc catcgtgctc
     9541 acgccgacga acaaagcaca gcgcctgctg atacagggtt catccgcaac cgaagtggcg
     9601 gaagtcgcgt tcggacgcac ctccgtcgag cttcacggaa aggcctggga ggtcgactac
     9661 tcgatactcc cgcggaaccc gctgcagatg accgtgacac tggcggcatc ggcagcgctc
     9721 gatctcaggt tgaccgaaat tcgctacggt ataccagtat cgaggtcgcc acgcgccccc
     9781 ggactcattc cgatgaacaa tgtactgaat cgcggcagcc gtttcgaatc agatactgtc
     9841 cacgtcagaa cgcactatcg actctgatga gtccgcgggc atttcacttc cgagtactct
     9901 tacggtgcgc tgaccggggc tgatcctgtc ccaaccacac tcggcgctgc cggggatgcc
     9961 gggttaacag tcccagtcgc gacactcggt gttccgcttg caatagacga cttggcaggg
    10021 ggatttccgc gatgcgccag ccacttcttg attgctccgc gcttcttgag cgcgaaccca
    10081 ccgccgagga tcaatgccag aatcaggaag taggtcaacc aggggcgagt cttgtccgca
    10141 aatgggtctt cgagatacct cttggcacca tcgggcagcg tggcgacttt ggtcaagctc
    10201 tgggcaaatc gcacattcat ggttgcccgg gtgttcaccg cccagccgtt ggcatcgagc
    10261 agagggccga gattgcgctt gccgagacgc agccaggcca gaaccatcga cggcccggat
    10321 atcagcagca aaagcccgac aatcgccagc ggcatctgcc accaggacag cccgagaaat
    10381 ccggtgatca cagaagccac agccgtacca atggcaccaa tggccaggcc gatcgccgca
    10441 aaaattccgg caaagcgacc gacatcaaac ggctgcgccg gtgccggggc accagcggtc
    10501 accgtcttac cagcttcggc aaccccactt gacatgcggg cctgcatttc cttgtcaccg
    10561 ctggccgcaa acttttcaac ctgctctgag atcatgcgtc ccagacgccg atagggcgac
    10621 cagaaagcct cgccaatgct gatcggatgt tcgacgatct tgacgatggt ggcatcccat
    10681 tcattgccgc gtcgatcgaa gaacacgccg ttccgcccga cgctgagctg ttcagcatca
    10741 ccagcggtaa acgccgcaac aatcgtcatc ttctcggaca tgccacgacg cacacagtcg
    10801 cagtatgcga gataggtatt acagagggcg gaaagcgagg catgcctgcc ggcatcttca
    10861 acgcgcagac acagctcgca cgctcgacca tcgatgtaga gggttcctgc ctgaaacatc
    10921 gcctgatgtt tgccggaaaa gaagtcgagg aaggtgacaa agttattgat caaggtcgcc
    10981 agatcgcgat gatacaacac cagtttttcg acttcagaaa tgctgtcgaa ttccgccttg
    11041 agagccagat cgtctgcgat cagagctcgc accctctttt catggtcacc ggcaatcaat
    11101 tctcggattc gtttcatacc aagcttttca acactgcttg acggcttttt tgccagccac
    11161 gactggtacg gagcgaaaca ctccaccaat ttctgcgagt cttcatgagt caactgcgtt
    11221 cgactgccga gcatcggttc gacgacttcg cgcttgaagc gtgccaggca gtcagcccac
    11281 gccggattga cacgatccgt cagcggaagt tcagcagtgg ccgacgggtg agcaagagga
    11341 agggctcgaa cggactcccc ggtagcggac agcatcgcca tcgtcggcag tggtggctcc
    11401 gcttgcgagc cagtcactcg ctctgccagc cgttcgtcaa aggccagtaa ccgacagcga
    11461 gtgaaaaagt cctctacttt cggcgcaagc tcctgccaca gcccgaaagc cgacgcggtg
    11521 ttctcaccga acggaagaac tcgtttggaa tcggcctcga gcagcttgta ccattcatcg
    11581 tgggcatgca gttccgccca gaaacggtca acaagcacag acgagacccc cggcaagcca
    11641 ctgcgatcgt tctccgagcc gacacacgag atcatctctt cgatcaacgt cttcagatct
    11701 tctttcgacg ccgccgtcgc cggtactatt ccatcgccat tcagccgcgt ctgcgcgaac
    11761 atggcgacca tactgcgcac atcgtcggca gtgatttctg tagcctgttc tttccccagg
    11821 gttttcagaa tctgtcgcat ggctgataac aaacgttttc catcagcgtg ctgctcgttc
    11881 accgccgaca acggcaagac cgctgaccgt ttcaagagat cgtcagggtt gcgcaatcgt
    11941 tcaccagccc aggcaacagc cgccagcact tcggccacgc gaatccgacc gtcctggtcg
    12001 gagtcaacga gatgcagcgt tttttcatcc agttcgacac ctttggtcgg acaactcaac
    12061 gtcgcccaga gtttcgaatc aagttgaccg agtgcgagca gatctgcgcc ggattcgata
    12121 ctgacctgat caagaccacc gcagcgatag aaccgccact ggtgcctggt tgttgatgcg
    12181 ctcgtcgttg gcatatgcag ccccttcaaa cttatcatta cgattgtatg atgcaccatg
    12241 agtggcttct tcacaacccc tgaacgatcc tccggtgtcc gaaccacaac gcgacgtttt
    12301 cgacatgagg aggtcgttct ctctctctca atgatgcacg cctcttgccg gacagctcaa
    12361 aagtttcact cacgccaaga ggaaaactga acagaatcaa atgaccctga aacgaggtaa
    12421 agacttttgc atcttcgccc tggctgacgt atgctttcca cacgttcagg atcgtcgaga
    12481 ggagtaccca tgcatcttca ttccagaatt tcggtcatcg cgctgcttgg tttcatcgcc
    12541 tgcctatgca ctccagcact tccagtctgt gctggggtgt tcggacaagt cgagcgcgac
    12601 tcgaacacgc cccctcctga gaacgacacc accactgagg gccgtccggc atccgatcag
    12661 agtgagccga cccgactgat gatgcgaggc gcttcttcca tgcccgcgaa aggcactctc
    12721 gccgaccggc aggagtaccg ttacgtcgtg acgttgccgc cggcgatgga cctcagcatc
    12781 gaactggaga gtcaaccggc gaacgccgct gttcccgaac tgctggaccg tgacgggacg
    12841 aaagtcgagt tacgcgatga aggcgaggga acctggatgg gcgctgtccc ggcaacgccc
    12901 gacggcaagg aggccgattt cacggtggtc gtgcacaaga aaccgaccgc cgcgaccgaa
    12961 gtttccttca tcctctctgt ccgagttccg gaacttggag acgaaggcgg aaacagcgaa
    13021 agcatgcaac aggaacccag catttcagcg ggcgactctt ctcaaggcaa gagcgacaga
    13081 cgaaccgtga tcgccaagtc cgggttgtca ctccgtgcga gacctgatcc gaaggcgacg
    13141 aagctcgcca cactgccgtt cggaaccgtg gtcgaggtgc tcgatatcga tggtccaccc
    13201 gacatgtatg aaggcatcaa tgcgcagtgg tacaagattc gggccaacgg caaagaaggc
    13261 tgggctttcg gcgggttcat cggcgagcca ggcgaaatgc cgacacctga gggttcagga
    13321 atgcgcggag ccatctttaa ccagccctgg gaagaacgca aggacggtat gcgcttcttc
    13381 gaactctctg atcccggcac ggaaaagctg aagctccaga acggcacgtg gctgacgctg
    13441 gaaaaaggaa aaacgagcgg caagttcgag ttcacgaacg aaggctatct gttctatgac
    13501 ggcgtgttca tctcacgcaa tttcgtcgct gaatacgtca gctcaaccga tgatccctcc
    13561 ggaaaaatgg ttccggaaat ggcgaccaag ctgatcttct ccccggcctc tccctctggt
    13621 cgacagatct tcttcacggc aacccgggac agtggctgcg ttaacgagta tctgctcaat
    13681 ctggacgaag gagtgattct gaacgtatcg atctcgaagt atggagttga tgagtggatc
    13741 gagtggtcga acgatggaaa atccgtccaa ctcagatacg aaacagaagg ttcgaggttc
    13801 atctacaggc tcgacatcaa gacaggaaaa gaaaccctgt tgaaagagga aaccatcgcg
    13861 gaatagcaca cctcgaacct ggacacctga tatcgtgtct cggtttcacg gcaacagaaa
    13921 accgggccgg cagagatcgc cggcccggtt ttcctttccc tgacttgctt actgctgcag
    13981 ggtcttgatg tactcgagcg cggactgcag gcgagcagac gcttcgtcgg tgtctttcac
    14041 gaccagacag aacttggtgt gcgaaagccg cttctcgacc tgtttgaaga cctctttgag
    14101 gtgcttggga gggtgaattt tgttgagggt cttgagcgcg tgattcacgt gttcaatggc
    14161 cggaaacatc ttcagatagc agacatcctt gccgcccagc gtgttcagcg ccagttcgat
    14221 gtccttcacg accagctcga gcagcttcag catggcttcc ttctgcaacg cggggtcttt
    14281 gtgaaacagc ttcttcaccc acgcgagaat ggcgcagtct gagccctgac cgccggactg
    14341 gtcagcctgt ccggcctgtt cactctgccc agcctgaccg ggatcaccgg ccggggcatc
    14401 gaccacaccg gccatcgccg tcggaaccac gaacaacact gccatgagaa cgaggacgca
    14461 cagagaacgg gaataattca taacgcctcc gggaataatg ttgcgaacca tgactcggcg
    14521 aatcgcaaat cacgtaccac cccgaaaacc ctgacgaaat cgagaatagt tgaggttatg
    14581 cgtctctcat gccggaattc tgacaaccga aacacgaatt ttcagctgag cttttccatt
    14641 ctattcatgt tgtcgcgtga cttcggacgg taggcttgcc cttgcatccg ggctccgtgc
    14701 cagatcaggc gctgaaacag catgccgaca ctcggcgact tgcgacggtt gtagcgaaag
    14761 gcgaactcat cgagataccc ctgcaggaaa gcccgcgaca gacgcccctg atgggttccg
    14821 agcaaccagc gacgaatcag accgaccacc cgtctcaccc gcggcaacgg cgtcgaggga
    14881 tcatcgccgg cagccacgat ctcgcagcga tatccttctt ccgccagtgt ttcatatccc
    14941 tcccaaccgt ccacttcaag ctgagttccg ggcttcactt catcgcgaat gaaccgcacc
    15001 agccctgccg cgggttcagt ggtgatatcg cgcaggcgaa tgcgaccaaa ctcgtcacca
    15061 ctggcgtcac tttccacagc aaccgcaatc agagccgaac gccagctcga ccctttcccg
    15121 gcttctgagc cggccaacca ggcatcatcg acctgcaccc agccctcgag cggctcccgg
    15181 tcgggacgaa ccatggcctg ccgcagtcgc cgcagccacg agaaggccgt ctgcatactg
    15241 ttcaacccca gcaaacgctt gaggccggca gcgctcaccc ccgtcttact ggtacaaatg
    15301 aaccacatgg cgagaaacca ggtacgaagc ggcagacgag accgatgaaa cagggtccct
    15361 gcggtcagag aggtctggcg atgacaaccc gcgcactcca gccagccacg gcggttcttc
    15421 caggcctggc catgaccgca ggcgggacag acaaaaccct cgggccaccg ttgttcgtag
    15481 agatagcgct cacaagccgc ttcggtatcg aaccagcgtt cgaattcggg aaaatccttc
    15541 ggaaaacggg ggtgttccgg tggctggtgg tgcaagacgc ttgtcatcac gaacagcata
    15601 acatgaatta aatggaaatg ctagaaagcg aaaccagaaa gacctgccga ttatccgcga
    15661 aaaaggagaa agcccccgac cggtcacccg gaagggggcg taaacgcaga gattgtcgat
    15721 tactcgggtt gtgacagcgc gagaagcttg ccgtcagtcc aggtgaactc catggtctga
    15781 ggtctgactt ttccggtcgc gggatccatc tcggttccag tcagtacgag atcaggtttc
    15841 tgatcgccat tcacgtcttt cacttccgcc gaccccattt tcatggtttc ttcccagatc
    15901 acctcgaaac cctgattcat cggcaccagc agggcaaatt ccttgaaccc gccgtttccg
    15961 ccgggatcaa gggtcagcag cagttgacgc gactcggtac cagccaggct cacgatctcc
    16021 cacttctcga gaatacggcc ttccgtctca aaaatcacct ttccctcgcc tgtccctgag
    16081 ggcggcatca gaaccagctt atcgaaacgt ccggtcgtct caggaggcgg agttccctca
    16141 accaccttga cgacaccgag gtcacccaga ttcaattctg ttggcataat tgccagaagc
    16201 ggctgagaca aatacccaac caggacaacg acccagatca tcatctccac tttcgccatc
    16261 ttcatctgct tcctcctgat caccgtccga aatccaaacc ttgacttacg gcctggcaaa
    16321 aagcactgga gcaccgcgca tcaggctgtt gcgatcttcg gccaccagat attcccccat
    16381 cttgcgaaac agcacgagat ctgtctggtc atctgccggt gcgtctgcgg tcacggtggc
    16441 gttcacgatc tcctgcagac gttcgcgcat atgctgcaga tgcagtttga ccgactccga
    16501 agagttggcc cccagctcga ccagacgccg tccgatctgt tccagaatcg gcacgaaaat
    16561 ctcgcgatcg agtcgcgacc gaatcccttg cgcatgcttg accagcagtt gcaggcgacc
    16621 acgaatcaag gattcacgga tcaaaacccg tccggctgcg gtcacaccga tcaaggcgat
    16681 ttcagatccg tacaagttgt tttcgagcaa taccccgttt tcatgaatga tatcgtcgac
    16741 aagattacct gtcagacgcg tctgcagttt ctcgagcgac tgtcgcaggg gtgaatcgga
    16801 catgcgtcca cacaaacgcg cgagataccg cagtacttct tcggtcgaaa acgcgaactt
    16861 gagtcgagtc atcaggctct cgagcggatc ggtgaccgag tcttcacacc agtacacctt
    16921 gtgaatctgg cgcgtgaact cgtgtcgttc gggaatcacg tctgcgtatg ccgcaagata
    16981 gtctgggtca tagagttcgt tttcctgctc cagagactct ttttcgagtg ctgcgaatcc
    17041 ataccgggct ttttcttcgg tcgacatcag tcgaaacagt gaggcaaaag ctgccgtttc
    17101 ggcccggcgg gcaaccgtcg accaccagac catccactcc tgcatgcgct gcttcgggtc
    17161 ttctgccgtg agaagctgat gccgcaaaag agcactctgc gtgcgcatca actcaccaat
    17221 cgcccgttcg agactgatga ttgccacgct gagatgggca tcggaccgag ccagacacag
    17281 gcagcgaaac agggccagca ggcggtcttc cggatacgca tacccatcga gcaatcgctc
    17341 gtagggctgt ggaaacaact cgcgctcgat ctttacttcc gcgaccggag tttcggcata
    17401 accgttctgg gtcagcacac acccacagag cagcaacagc atcagtacct ggcgaaccac
    17461 tggcgacata cggcctccct cgcaacgaca gtcccttgga cgggtcggtg agagtatacc
    17521 tcaaccccgt ccattccgca gcaccaccaa tcagggcaaa cgggaagtat ttcctcgagt
    17581 gacgggcact gaaactctcg tgtgtcagcg aagcgttcgc ggttgccctg caccaccgat
    17641 cgtcatcgtt ctgcacgttt cctgaagcgg ttgcgatata ctgcgactcg tggaacacgt
    17701 actgtctctt ccgacggtct ctcgtcgagc tctgttgctc ttctggctgg tcgtgctttg
    17761 tgttcccctg ctgtttctgc gttccagcat ggtcagtttg atccgcgagg atgaacgcaa
    17821 cctcaccgag caggcccgac gcgccttgcg cgaggaaatg acggcattcc tcgacgacct
    17881 ttccttgccc aaacagattc tgaactctct gcgttcggtt gagagccgct cctcacgcct
    17941 tgccaccctc accgggttga aacagttgga ttcagagcag ggaatcgcgg atctgctgct
    18001 gttggaactg gctcgcgagc tcccagccga acctctgttt ctgctgattg accccgggca
    18061 cggacgacct cccgagatcc gctataacac ccgcctcttc accccattgt cggccccatc
    18121 gcgccgcctg gcggagttgt ggctgcgttc gctgaaagac gtgctgtcac cggaagagag
    18181 gcaacgtctc gatcgtacgg tctgtaacct gctcggcgaa ctcgtttcca cgaacctgcc
    18241 acccgatcag gtacaagaga gtttttccgt tcgcctcggc cacgaacggc tgctgctcgc
    18301 aggtcgaacg ctgactgaga ccccgacggc tcctttccga cgctggatca ttgcgtttcg
    18361 cgaccgggat ttccgcctca ctcagatcat cagacaggct gtctctcagt ctctcagaca
    18421 tcagtgtcgc aggcaggtca gattctccac caccgacgag agtggagcca cgtttgatct
    18481 gactcgggca cacctcaggt attcgctccc catgccccga ctcctctttg ctcagggaac
    18541 cgacatcccg caacgcggaa tcgaatcccc ccttccccgg ccgcagc
//
LOCUS       DAORHD010000002        13709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_3546_length_13709_cov_4.513329, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000002 DAORHD010000000
VERSION     DAORHD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 13709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13709
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3546_length_13709_cov_4.513329"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..356
                     /locus_tag="PKO06_00080"
     CDS             54..356
                     /locus_tag="PKO06_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV68060.1"
                     /translation="MSEMNTSAVRAERLHRRFATARKTEGLWASLKNLLAPVYDQIEA
                     VTDLDLEIGVGELVGFLGPNGAGKTTTLKMLSGLIPPSDGIVQVLALTHFSGTMDS"
     gene            374..1048
                     /locus_tag="PKO06_00085"
     CDS             374..1048
                     /locus_tag="PKO06_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV68061.1"
                     /translation="MGQKQNLWWDLPPAETLALHRELYDIPRDDYHCRRDELVDMLEI
                     GDCLDIQARKLSLGQRMRCELAVALLHRPQVLFLDEPTIGLDILMQKKIRDFLLAYHA
                     RFHPTILLTSHYMEDVEALTKRIIVINRGRKVYDGALSQLTAAARLQKTLTVTFADLR
                     PESDFEKWGTLIEREGTRYRLRVPREKAASAAAELYSSGGVVDLTLEEAPLEEALAGL
                     FARPRD"
     gene            1054..1857
                     /locus_tag="PKO06_00090"
     CDS             1054..1857
                     /locus_tag="PKO06_00090"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNV68062.1"
                     /translation="MALKNSLSRLFLAHLQVTLAYRGQMLLWTMQEMLIPLVLMSAWL
                     SIERVEGNPYGPGDYLLYYLALPIVRNLSDCWTVHSLPAQVREGVLSRELLKPMHPLW
                     SHLFEHLAMKILQLGTFLVPLSALGWYYFDQLPALHLSLAKICLLGLVVLLAMALRFA
                     MNTTLAFTGFWIEQVDSLHLVINSAFWAMMAGMVVPLETLPPAFRAVADLLPYRYTLS
                     FPLEILRGGLPGVEILRGLLIGLLWLALLAWTGRGLWRRGLREYTAYGG"
     gene            1859..2647
                     /locus_tag="PKO06_00095"
     CDS             1859..2647
                     /locus_tag="PKO06_00095"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNV68063.1"
                     /translation="MLRLMRILLHFWKAGLMQAMEYRAGFLIGMFANTCDFTFGLIQY
                     VLFFSAAKSIAGWETSQMLTLYAIFMTMFSLHFIFLYPNLEAMSRLVNRGELDLALLK
                     PASAQVILSFRHLSFDEFGSFLASQALLLSLLVSGSISPSWHGLAGFGVAVVCGLSFI
                     YASFLILMSLTIIFEKMHNTADLLWSLFSLTRYPVDVYPRWMRSLFLAVIPIGFISSV
                     PASMLTRGSDWPVLLTGVGLSIVFLVLARRAWVAALRGYCSAGG"
     gene            complement(2650..5535)
                     /locus_tag="PKO06_00100"
     CDS             complement(2650..5535)
                     /locus_tag="PKO06_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV68064.1"
                     /translation="MTLLAPAWLVLIVPLWFLTGRLPLPSVLIHRLRLAALACLVLAM
                     CGPAVRLPMREGTVVVVADRSFSMPTGVDGQLKESVELLHQALPPNHRLAVVSFGERV
                     VVEHPPSTGKFPGFLAQVDRNGSQLGEAIERSISLIPREGTGRLLVISDGRATGRDFS
                     ESAFKSAARGIPIDFRLVQRELAGDLAIQNVEAPQQVAAGEAFFVHAWINSPMSQNVT
                     VELYRDKTLVSRKQLEALPGLSAVTFRDRAEEAGTHRYFLKVVGSHPDPVDENNTARL
                     LIGVAGERPLLALSGTADQGYARLLQRNKVPVRIVNPAEFSFQLDELADYAGVIIENL
                     PAGRIGERGMETLSAWVKNTGAGLLMTGGRNSFGPGGYFRSPLEPILPVSMELRREHR
                     KLSMALVVVLDRSGSMMAPAGGGKTKMDLANLGAVEAIELLNNLDEFGCFAVDTAPHI
                     MVPLQPLDNKSKVRDLLLRIRSEGGGIYVYEGLAAAHKMLKGAKAGTRHVILFSDTCD
                     SENPGDYLKLLKQMTDEGITVSVIGMGVETDCDGALLRDIAEKGNGRIFYSDRPEELP
                     RLFAQDTIVAARSTFVETPVPIQFEPTLQQITGRSFSPDRPAGGYNLCYLREGAQQHA
                     VSGDEYHAPIISAWQVGIGRSMAVTLEPNGPFTGDLKYWSDFGEFHSSLARWVAGKSG
                     TLFRDQPVTQTVANGYCQIQIHLDPERRHDPFAQPPRLLTLRSASGESTQTEERRFEW
                     ADPDTLSISLPLKGGQTAISTLDAPGGDPLPLSPVCLPYSPEFRPTGENEGQRNLEKM
                     ARMSGGKERIDLASIWNELPQTRRYLNIAPWLILLALILFLLEVLERRTGLVATLRAP
                     AAIRDWLERRRRVSEAEPVTAAPVLPSALSQAAAAGKNAIEARRDIPSTQPTLASQIG
                     NLVAPAPEPVDPAKPVPTAAPASPKKEATGIGSALKRARNEARKRQD"
     gene            complement(5532..7292)
                     /locus_tag="PKO06_00105"
     CDS             complement(5532..7292)
                     /locus_tag="PKO06_00105"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNV68065.1"
                     /translation="MPQFAFPAFFLALPLAAAALVAIYWLRNRFNPIRVSSLLLWQSQ
                     KEARESGVTLHRLQTPLLFFLELLALAAMACAAATPLLETTQEQRPYVIILDDSLSML
                     SGGNDSPREKSLQQIRKELEGSISYPVRFLLAGPEPVFLDSPARNRAEVMLALESWRC
                     RSPRADLAAAIALAEETLGGNGRILVFTDHQPPGTTESGKTRWLAFGQVRPNLAIVNA
                     VRGGSESRDRLLLELANYSSDSQTCTLIMTGLSTETQQVTVKLNAQERQRREFEIASS
                     ASTVKVQLTSPNDALSTDNDVVLLPRSAPRVKVAMNFKNGILKSQIERALISSGHIVP
                     ATDEPDLFLTDDQTVVPPSPGAWLVQFLPHTEALSYTGPFFIDPVHPLTQGVTLAGVV
                     WGAGKSELPGRHLLSVGNVPLISEQDIGTSRTIRIKLRPDLSTLHLSPAWPILLYNLV
                     DWRIRFLPGPTQPNLRLFQEVSFTVSPGIESLSGSTPDGRTLTLPVFAQGVSFRPEAP
                     GLYSFSGHTDSWQLSVNAFEPLESDLASATTGTWGSWTDAATLEKDFRPMTDAVLAFV
                     LVILALHQLAVFRRPKGIRI"
     gene            complement(7295..8167)
                     /locus_tag="PKO06_00110"
     CDS             complement(7295..8167)
                     /locus_tag="PKO06_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV68066.1"
                     /translation="MNSEYRQFLREGEQAASRYALTSPRRLPRGMSGNILGSGAGSSL
                     EFLDHREYQPGDDLRHIDWSAYARTDKLIVKLFREEISPHCDILIDGSCSMALAETQK
                     ARSTVALSALLGQAAQNADCSCRAWFVTDQVRPLPNGHERPSLWEGLDFRFPGNPGES
                     MLRQPPECKPHGIRILVSDLFWAIDPLPLLQRLAEKAAALFVVQILAQSDLGPTERGN
                     IRLIDSETGLEKEIFLDDPTIQAYRDSLQRLQHNWNRSCSQVGARMVTVIAESLLQTW
                     DLQELVSGELLKVG"
     gene            complement(8318..10876)
                     /gene="hrpB"
                     /locus_tag="PKO06_00115"
     CDS             complement(8318..10876)
                     /gene="hrpB"
                     /locus_tag="PKO06_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNV68067.1"
                     /translation="MIRSTGLPVEGFIVEIQNALCASNNLVLVAAPGAGKTTVVPLHL
                     FDEPWMQGKRTLMLEPRRIATRAAARRLAALHGSTLGQTVGFQIRFDRCIGPHTRIEV
                     LTEGLLTRRLQQDPFLEDVGLVIFDEFHERSLHADLGLALCREVQQTVRPDLRLLMMS
                     ATIEAGPIAAFLGQCPTLQIPGRQYPVETLHLERPSTLSLIERVAQAVERLFPAVPDQ
                     GDLLVFLPGEAEIYRVKELLETRGFDRQAAILPLHGTLPVDRQDEVLRPGTRRRIILA
                     TNVAETSLTIEGVRGVLDSGLARQVRLDPRTGLERLETTTISLANARQRTGRAGRLGP
                     GICLRLWTADEERSMPNEIVPEIRRVDLAHLALELAAWGKPDPLKLDWLEAPPVPAVG
                     AARQLLQQLGAITSDGGVTVLGRRMAALPLHPRIARLLLFAEQLGQGNLGADIAALLS
                     ERDILEGGRREARESSRSDLFIRLEALDSARRQHFRGSLSQIDLRAAAAVDRVAEQLR
                     RYLTSVPAEQKRKAVPFEEAVGRIPLPAFPDRIARRRGKPVPGTRTGYVLADGRGMRL
                     SESSSVQQSEFVIALAVDGTERGETREGLIHLAGEIDPAWIRELLSADITTVREPVFD
                     PQRRSFSIRRRVFYRDLLLDESTENMTADDRNLVEQQLAEEAGKRLNEALTFDEEFEQ
                     LRFRLRLLAHHDSSLAIPEIDEDWIRTRLPGLVMGKRSFADLKAVSLCEFTLAQLDHR
                     IVGSLQRHVPERFLLPSGRKVRLQYQLDGPPVLAVKLQELFGQTETPKLMQGRVSVLI
                     HLLSPAGRPLQITQDLRNFWLTVYPGLQRQMRAKYPKHSWPDDPFTSRPPPLKR"
     gene            complement(10873..>13709)
                     /locus_tag="PKO06_00120"
     CDS             complement(10873..>13709)
                     /locus_tag="PKO06_00120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68068.1"
                     /translation="RWLVTCLMFFGLPFLLVAYGLGTVWEIQRSEARDRLFSRLDEYL
                     LTIRKFDNTVEFFDDFFHRCLRRLRKIDEDNSRGKSRAYLKLVLWNLHRRFPGVFQTT
                     MVTEKGEPDPRYSPTAAPKLIVRRLVETIREMQFSPRGDYSTTRELVWPLIRGYIGPV
                     AEVGKFDAFRQRLIEANTDAKNRWFFYLISRSSGLLAHISQTPDWDIIPARDRMRRLR
                     RQVRDPEVKFGLITLDRQSQLRGTLALAIGEHERTLSDHVEVGRHLVSVMPYASKARI
                     WASIPTDRLPNFLWHRLCLLAIGSLVFGLFSWLAYRFIVLETTFRFTMRNRLILLFGF
                     ASGLPLMVLGFYGWDYLHDKFRTRIREAHDIADRNLQILDGRFPLQRGIMERLCQRIG
                     HQVEAQLLHGHAVDMNRLDRLSRRLGRAEFLLIDVKGRIRSSSDARKEHRSANSQMRK
                     LFGIVAKSVLKQIDKEGSHQITSSEMLVSSVPGIDLALNPLLKGLGSITPINFADFHV
                     WVYFLPIRGASGNIEYLFSATWDKFGLENYFLQTHLTGINRHRPDTRFFGFHTASEKL
                     YPEWKSRPQQLLNFMTNLRRQQGTTTETLVWRGRNYLLTGIKPKELSQFFLLAIENAD
                     PIENEIKHLRLRLSLFTIIAVVFSLGLGLTLSRQFLTPLATLTQGVVAIQERQFRHRV
                     PSLGENELGDLALTFNRVMEGLSDLEVARIVQESLFPAAEIHIGPYRASGRSVSASEL
                     GGDYFDMQKLSDDRLLILIGDVSGHGVPAALVMAMAKAFIEYQCSLSTIPTPDQLLAG
                     LHLVLFRTLKRRRTMTCFLGLLDTTTHQLTYANAGHNYPFLFRRGEPARNLTLPSFPL
                     GSRQNISVKTAEELLVSGDHLLLYTDGFIEAQTEGDSIGYQRVAEEVPLLFTDDPSAS
                     IDRILTWHKRLSQDITQADDITLVILSRAP"
BASE COUNT         2842 a         3728 c         4241 g         2898 t
ORIGIN      
        1 gagtctgagg gtctggtttg acaattctcg gagtcgagag ctaacgtgct cgcatgtcag
       61 aaatgaacac cagcgcggtt cgtgccgagc gtttgcatcg gcgcttcgcg acggctcgaa
      121 agaccgaggg gctgtgggcg agtttgaaaa atctgctggc tcccgtctac gatcagattg
      181 aagccgttac tgacctggat ctcgagattg gcgtcgggga gctggtcgga tttctcgggc
      241 cgaacggtgc cggcaaaacg accacactga aaatgctttc cggtctgatt ccaccatccg
      301 atgggattgt acaagtgctg gcgttgaccc atttcagcgg gactatggat tcctgaaacg
      361 catttcgctg gtcatgggac agaagcagaa tctctggtgg gatctcccgc ctgcggagac
      421 gctggcatta catcgcgagt tgtacgatat tcctcgagat gactaccatt gtcgtcgaga
      481 cgaattggtc gacatgctcg aaatcggtga ctgcctcgac attcaggccc gcaagctttc
      541 gttgggacaa cggatgcgct gtgaacttgc ggtggcgctg ctgcatcggc cgcaggtgct
      601 gtttctcgat gaaccgacca tcgggctcga cattctgatg cagaagaaga ttcgcgactt
      661 tctgctggcg tatcatgccc ggtttcaccc gaccattctg ctgacctcgc actacatgga
      721 agacgtcgag gccttgacga agcgaatcat cgtcatcaat cgcgggcgta aagtatacga
      781 tggcgctctg agtcagctga cggccgctgc tcgtttgcag aagaccctga ccgtgacgtt
      841 tgccgactta cgtcctgagt ccgatttcga aaagtgggga acgctgatcg agagggaagg
      901 aacgagatat cgtctgcgag tgccgcgtga gaaggccgct tcggcggcgg ctgaattgta
      961 cagctctggc ggagtggttg atctgacgct cgaagaagca ccgctcgagg aagctctggc
     1021 tggtctgttc gctcgtcccc gggattgagt ttcatggcgc tgaaaaactc gctgtcgcgc
     1081 ctgtttctgg cacacctgca ggttactctt gcctatcgag gccagatgtt gctctggacc
     1141 atgcaggaga tgcttattcc gctggtgctg atgagtgcct ggctgtcgat cgaacgagtc
     1201 gagggaaacc cttatgggcc gggagactat cttttgtact atctggcatt gcctattgtt
     1261 cgcaatttga gcgattgttg gactgtgcat agtcttccgg ctcaggttcg cgagggggtg
     1321 ctcagccgag aattgctgaa gccgatgcat ccactctgga gccatctgtt cgaacatctg
     1381 gccatgaaga tactgcagct gggtacgttc ctggtgcctc tcagcgctct gggctggtac
     1441 tacttcgatc agctgccggc actgcatctg agtctggcga aaatctgcct tctcgggttg
     1501 gttgtgcttc tggcgatggc cctccggttt gccatgaata cgactctggc tttcaccggt
     1561 ttctggatcg agcaggttga ctcgctgcat ctggtgatca actcggcttt ctgggccatg
     1621 atggccggaa tggtggtgcc gctcgagacg ttgccgccgg cattcagagc ggttgctgat
     1681 ctgcttccgt accggtacac gctgagcttt cccctcgaga ttctccgggg tggtcttccc
     1741 ggtgtcgaga ttctgcgcgg gttgctgatc ggtctcctgt ggctggcgtt gctggcgtgg
     1801 acaggtcgag gtctctggcg acgcgggttg cgcgaataca ccgcgtacgg aggatgagat
     1861 gttgcggttg atgcgcattt tgctgcactt ctggaaagcc ggactgatgc aggcgatgga
     1921 gtatcgagcc gggtttctga tcggcatgtt cgcgaacacc tgcgatttca ccttcgggct
     1981 gattcagtat gtcctgttct tctccgcggc caagtcgatc gccggctggg aaacctctca
     2041 gatgctgacg ctgtatgcca tcttcatgac catgttctcg ctgcatttca tctttctgta
     2101 tcccaatctc gaagccatga gtcggctggt gaaccgcggc gaactcgatc tggcgttgct
     2161 caaaccggca tcagcacaag tcattctttc gtttcgtcac ctctcgttcg atgagttcgg
     2221 cagttttctc gcctcgcagg ctctgctgct ctccctgttg gtcagcggct cgatcagccc
     2281 ttcatggcac ggactggcag ggttcggggt cgccgttgtc tgtggtctgt ccttcatcta
     2341 cgcgagtttt ctgattctca tgtcgttgac catcatcttc gaaaagatgc ataacacggc
     2401 tgatctgttg tggagcctgt tcagtctgac ccggtatccg gtcgacgtct atccgcgctg
     2461 gatgcggtct ctgtttctcg cggtcattcc tattggtttt atctcgtcgg ttccggcttc
     2521 aatgctcacg cgcggcagtg actggcctgt actcttaacg ggtgtcggct tgtcgatcgt
     2581 cttcctcgtt ctggcgcgtc gagcctgggt ggccgctttg cgcggatact gctctgccgg
     2641 gggctgagac taatcctggc gtttgcgggc ttcgttgcgg gcgcgcttga gcgctgaacc
     2701 gatgccggtt gcttccttct tgggtgaggc gggtgccgct gtgggaacag gtttggcggg
     2761 atcgacaggt tcgggggccg gcgcgaccag attgccgatc tgcgaggcga gtgtgggctg
     2821 tgtcgaggga atgtcgcgtc gagcctcaat ggcattcttc cctgctgccg ctgcctgtga
     2881 gagcgcactg ggaagcaccg gcgcagccgt gaccggctct gcttcggaca cgcgtcgacg
     2941 acgttcgagc cagtcgcgaa tcgctgccgg agcacgaagt gtcgcaacca gaccggtacg
     3001 tctttcgaga acttcaagca gaaacagaat caatgccagc agaatcaacc agggagcaat
     3061 gttcagatag cggcgcgtct ggggcagttc gttccagata ctggcgagat cgatgcgttc
     3121 cttgccaccg ctcatgcggg ccatcttttc caggtttcgt tggccttcgt tctcgccggt
     3181 gggacgaaac tccggagaat agggcaaaca gacgggggat agcggcaacg gatcaccacc
     3241 gggagcgtcg agggtcgaaa tggctgtctg accgcctttc agaggcaggc tgatcgacag
     3301 ggtgtccggg tcggcccatt cgaaccgtcg ttcttcggtc tgagtcgact ctccgctggc
     3361 ggagcgcagc gtcagcaggc gtggtggctg agcgaagggg tcgtgacgac gttcggggtc
     3421 aaggtgaatc tgaatctgac agtacccgtt ggcgacggtc tgcgtgacgg gctgatcacg
     3481 aaacaacgtg ccgctctttc ccgcgaccca gcgggccaga ctgctgtgaa attcgccgaa
     3541 gtcgctccaa tacttcagat cgccggtaaa cgggccgttg ggttcgagcg tcacggccat
     3601 agatcgacca atgccgacct gccaggctga gataatcggg gcatgatact cgtcaccact
     3661 gaccgcatgc tgctgagcgc cttcgcgcag gtagcagagg ttgtagccgc cggcggggcg
     3721 atcgggggag aaactgcgac cggtgatctg ttgcagcgtc ggttcgaact ggatgggaac
     3781 cggagtttca acaaaggtcg atcgcgcggc gacgatcgtg tcttgggcaa acaatcgcgg
     3841 taactcttcc gggcggtcgg aatagaaaat gcgaccgttg cctttttcgg cgatgtctcg
     3901 cagcagagcg ccgtcgcaat cggtctcgac tcccatgccg ataacactga ccgtgatgcc
     3961 ttcgtcggtc atctgtttca gcaacttgag ataatcgccg gggttttcgg agtcgcaggt
     4021 gtcagaaaag agaatgacgt ggcgcgtacc ggctttcgca cctttcagca ttttgtgagc
     4081 cgcggccaga ccttcgtaga catagatccc gccgccttcc gatcgaatgc gcagcaacag
     4141 atcgcgaacc ttggatttgt tgtcgagcgg ttgcagcggc accatgatgt gcggcgcggt
     4201 atcgaccgcg aaacaaccga actcatcaag attgttcaac agttcaatgg cttccaccgc
     4261 accgagattg gcgagatcca tcttggtctt gccaccgccg gctggagcca tcatgcttcc
     4321 cgaacggtcg agtacgacca cgagggccat cgaaagcttg cgatgttcgc gacgcagttc
     4381 catggatacg ggcagaatcg gctcgagcgg tgagcggaaa tagccgcccg gtccgaatga
     4441 gttccgtcca cccgtcatga gaagtccggc acccgtattc ttgacccagg ccgagagggt
     4501 ttccatgcca cgttcgccga tacgccctgc cggcaggttt tcgataatga cccccgcata
     4561 gtccgcgagt tcgtcgagtt gaaaactgaa ctctgccgga ttgacgatgc gcacgggaac
     4621 cttgtttcgt tgcagcaggc gcgcatatcc ctggtcggct gtcccggaaa gggccagcag
     4681 ggggcgttcg ccggctacgc cgatcagcag acgggcggtg ttgttttcgt cgaccgggtc
     4741 gggatgcgaa ccgaccacct tgagaaaata ccggtgcgtg ccggcttctt ctgcccgatc
     4801 gcggaaggta acggcggaga gaccgggaag tgcttcgagt tgcttgcgac tgacgagggt
     4861 tttatcacgg tacaactcga ccgtgacgtt ctggctcatc ggagaattga tccaggcatg
     4921 gacaaagaag gcctcgccgg cggcgacttg ttggggggct tcgacgttct gaatcgccag
     4981 atcgccggcc agctctcttt gtacgagtcg aaaatcgatg ggaatgcctc gagcagcgga
     5041 tttgaaggct gactcggaaa agtctcggcc ggtcgcgcgg ccgtcggaga tgaccaggag
     5101 tcttccggtt ccctctcggg gaataaggct gatggagcgc tcgatggctt cgcccagttg
     5161 ggagccgttt cgatcgacct gagccagaaa tccgggaaac tttccggtcg aaggcggatg
     5221 ttcgacgacc acgcgttcac caaagctgac aacggccagg cggtgattgg gaggcagtgc
     5281 ctgatgcagc agttcgaccg attccttgag ttgaccgtcg actccggtcg gcattgagaa
     5341 gctgcgatcg gcgacgacga cgacggttcc ctcgcgcatg ggcagtcgca cggcagggcc
     5401 gcacatggcc agcaccagac atgccagagc cgccaacctc agtcggtgaa tgagcaccga
     5461 agggagcggc agacgcccgg tcagaaacca gagcggaacg atcaggacca gccaggcagg
     5521 ggcaagaagg gtcatatgcg gatccccttg gggcgacgaa agacggcgag ctgatgaagg
     5581 gcgagaatga ccaggacaaa tgccagaacg gcatccgtca tggggcgaaa atccttttcg
     5641 agggttgcgg cgtcagtcca gcttccccac gttccagtgg tggcactggc caaatcagat
     5701 tcgagcggtt cgaacgcgtt gaccgacagc tgccaggagt cggtatgccc ggaaaacgaa
     5761 tagagcccgg gagcttcggg acggaacgac acgccctggg caaagaccgg gagagtcagc
     5821 gtgcgaccgt ctggagtgga gcccgagaga gactcgatgc cgggcgagac cgtgaacgac
     5881 acttcctgaa agagtcgaag attgggttgt gttggaccag gtagaaagcg aatgcgccaa
     5941 tcgacgagat tgtacaatag aatcggccag gcgggtgaaa gatgcagtgt cgatagatcg
     6001 ggccgcagtt taatacggat ggtgcgcgac gtgccgatat cctgttcgct gatcagtggc
     6061 acgttgccga cgctcagcag atgccgcccg ggaagttctg acttccccgc gccccagacc
     6121 acacccgcca gagtgacacc ctgcgtcaga ggatgcacgg ggtcgatgaa aaacggaccg
     6181 gtataggaca gcgcttcagt atgcggaaga aactgtacca gccacgcacc gggagaaggg
     6241 ggaacgacgg tctgatcgtc ggtcagaaaa agatctggct catcagttgc ggggacaata
     6301 tgaccacttg aaatgagcgc tcgttcgatc tgtgacttga gaatgccgtt cttgaagttc
     6361 atggcgactt tgacccgtgg tgccgatcga ggcagaagaa ccacgtcgtt gtcggtcgaa
     6421 agcgcatcat tcggagaagt gagttgtact ttcacggtgc ttgcggatga agcgatctcg
     6481 aattcgcggc gttgccgttc ctgggcgttc agttttacgg taacctgttg tgtctcggtc
     6541 gagaggcccg tcatgatgag ggtgcaggtc tgactgtcag aagaataatt ggccaattcg
     6601 agaagcagcc ggtctcgact ctctgagccg cctcgcacgg cattgacgat cgccaggttg
     6661 ggacgaacct gaccgaacgc gagccatcgc gtttttcccg attcggtcgt acccggtggc
     6721 tgatgatcgg tgaagacaag aattcggccg tttcctccca gggtctcttc ggccagagcg
     6781 attgcggccg ccagatcggc acggggtgaa cgacaacgcc atgactccag agccagcatg
     6841 acttcggcgc ggtttcgcgc gggtgaatcg agaaagaccg gctctggccc ggcaagcaga
     6901 aaccggacag gatacgagat acttccttcc agttcctttc tgatctgctg cagagatttc
     6961 tcacgaggag agtcattgcc gccggaaagc atggagagcg aatcatcgag aatgatgaca
     7021 tagggtcgct gctcctgggt cgtctcgagt aagggggtcg cggccgcgca ggccatggca
     7081 gccagggcca gcaactcaag aaagaagagc aatggcgtct gcagacggtg cagggtcacg
     7141 ccgctctcgc gggcttcttt ctgtgactgc cagagaagca gcgaagagac gcggatgggg
     7201 ttgaatcgat tgcggagcca gtagatcgcg accagagctg cggcagcgag cggcagggcc
     7261 agaaagaagg cgggaaaagc gaattgcggc atgctcaacc caccttcaac aattcgccgc
     7321 tgacgagttc ctgaagatcc caggtttgca gcagggattc ggcgatcacg gtcaccatgc
     7381 gggcaccgac ctgtgagcag gaacgattcc agttgtgttg cagtcgctgc agcgagtctc
     7441 gataggcttg aattgtcggg tcatcaagaa agatctcttt ttcgagcccg gtttcactat
     7501 cgatcagtcg aatgtttccg cgttcagtgg ggccgagatc actctgcgcc agaatctgca
     7561 ccacgaaaag cgccgcggcc ttctcggcca gtcgctgcag caatggcagc gggtcgatgg
     7621 cccagaacag atcgctcacc agaatccgta ttccgtgcgg cttgcactcg ggaggctggc
     7681 gcaacatcga ttcgccgggg ttcccgggaa atctgaaatc gaggccttcc cagaggctgg
     7741 ggcgctcgtg gccgttcggc agcggtcgca cctgatcggt gacgaaccac gcgcgacacg
     7801 aacagtcggc gttctgcgcg gcttgcccga gtaacgcgct cagagcgacg gttgatcgcg
     7861 ctttttgggt ctcggccagt gccatcgaac acgagccgtc aatcagaatg tcgcagtgcg
     7921 ggctgatctc ttcgcgaaac agcttgacga tcagtttgtc cgtgcgtgcg taggcgctcc
     7981 agtcgatgtg gcgcagatca tcgcctggtt gatattcgcg atgatcgaga aactcgagcg
     8041 agctgccggc cccgctgccg agaatgttcc cactcatgcc gcgcggcaag cgtctgggcg
     8101 aggtcagggc atagcgtgat gccgcctgct cgccttctcg cagaaattgt cgatactcgc
     8161 tgttcattcc tctgaccgaa tcacgtggga gatgaagtcg agccagtata caccatcacg
     8221 acgaaccccg caggctcgtt gcaccatgta tctcctgctg atcagacgtg tattctgctc
     8281 cattgaacga tgggtcacca tcacgagaat cccgtgatca gcgcttgagt gggggcgggc
     8341 ggctggtaaa cgggtcgtcg ggccaactgt gtttcggata cttggcgcgc atctgtctct
     8401 gcagaccggg atatacggtg agccagaaat tgcgcaggtc ttgagtgatc tgcagggggc
     8461 gaccagccgg tgacaacagg tggatcaaga ccgaaactct tccctgcatg agcttcggag
     8521 tctcggtttg tccgaacagc tcctggagct tgaccgccag caccggtggc ccgtcgagtt
     8581 gatactgcag acgaaccttt cgaccagacg gaagaaggaa tcgttccgga acgtgacgct
     8641 gtaacgatcc aacgatgcga tgatcgagtt gcgcgagcgt gaattcgcag agagaaaccg
     8701 ccttcagatc ggcgaaactg cgttttccca tgaccaaccc ggggagtcgg gtgcgaatcc
     8761 agtcttcgtc gatttcggga atcgcgagcg agctgtcgtg atgtgccagc aggcgcaatc
     8821 tgaatctcag ttgctcgaac tcttcatcga aggtgagcgc ttcattcagt cgcttgcctg
     8881 cctcttcggc caattgctgt tcaaccaggt ttcgatcgtc agccgtcatg ttttcggttg
     8941 attcgtcgag gagcaggtcg cgatagaaca cgcgtcgtcg aatgctgaag ctgcggcgct
     9001 gcggatcgaa caccggctca cgtaccgtgg tgatgtcagc ggacagcagt tctctgatcc
     9061 aggctgggtc gatttcaccg gcaagatgaa tgagcccttc acgagtctca ccccgttcgg
     9121 tgccatccac ggccagggca atgacgaact cgctctgctg cacggaactg gattcggaaa
     9181 gcctcatgcc acgaccatca gcgagaacat agccggtacg cgtgccggga actggtttcc
     9241 ctcggcgtct ggcgatgcga tcgggaaacg cgggtagagg aatgcgaccg acagcttctt
     9301 cgaagggaac ggcttttcgc ttctgctcgg caggtacgct ggtgagatat cggcgcaact
     9361 gctcggcaac ccgatcaacc gctgcggccg ccctcaggtc gatctgtgag agtgagccgc
     9421 gaaagtgctg acgccgagca ctatcgagcg cttccagtcg aatgaacaga tcgctgcgac
     9481 tcgattcacg cgcttcgcga cggccgccct cgagaatgtc acgctcactg agcagggccg
     9541 cgatatccgc gccgaggttt ccctgaccga gttgttcggc gaacagcaac agacgtgcaa
     9601 tgcgcgggtg cagcggaaga gccgccattc gccgcccaag cacggtgact ccgccgtcgc
     9661 tggtgatggc tccgagttgt tgcagcaact gcctggctgc gccgaccgca ggaactggtg
     9721 gtgcctcgag ccaatcgagc ttgagtgggt ctggtttccc ccaggcggcg agttcgagtg
     9781 ccagatgtgc cagatcaacg cggcgaatct ccggtacgat ttcattcggc atcgaacgtt
     9841 cttcatcagc ggtccatagc cgaagacaaa tgccgggtcc gaggcgaccg gcacgaccgg
     9901 tgcgctgacg tgcgttcgcc aggctgatgg tggtggtttc cagacgttcg agcccggtgc
     9961 gcgggtcgag gcgaacctgt cgcgccagcc ctgaatcgag cacaccgcga acaccttcga
    10021 tggtgagcga ggtttcagca acgttcgtgg caagaatgat gcggcgtcgg gttccgggtc
    10081 tgagaacctc gtcctggcga tcgacgggca gcgtgccgtg cagcggcagt atcgccgcct
    10141 gacgatcgaa tccgcgagtt tcgagcaact ccttgacgcg gtagatctcg gcttcgcctg
    10201 gcagaaagac cagcagatca ccctgatcgg gcacggccgg aaacagccgt tcgaccgcct
    10261 gagccacacg ctcgatcagg gaaagcgtgg acggtcgctc aagatgcaac gtctcgaccg
    10321 ggtactgccg gccgggaatc tgcagggttg ggcactgacc gagaaatgcg gcaatcggtc
    10381 cagcttcgat agttgctgac atcatgagta gacgcagatc cgggcgaacc gtctgttgta
    10441 cttcccggca cagggccaag ccaagatcgg cgtgaaggct gcgttcgtga aactcgtcga
    10501 agatcacgag cccgacgtcc tccaggaatg ggtcttgctg taatcggcgc gtcaacaacc
    10561 cttccgtgag cacttcgatg cgtgtgtgcg gcccgataca gcgatcgaaa cgaatctgaa
    10621 atccgaccgt ctgtccgagg gtgcttccgt gaagcgcggc caggcgtctt gccgcggcac
    10681 gggtggcaat acgtcttggc tccagcatca gggtgcgctt gccctgcatc catggttcgt
    10741 caaacagatg cagtggcacg accgtggttt tcccggctcc gggagcggcg acgagcacca
    10801 ggttgttcga tgcgcacaat gcgttttgga tctcgacgat gaagccctca acgggtaaac
    10861 ctgtgctgcg aatcatgggg cgcgcgagag tatgacgaga gtgatgtcgt cggcctgggt
    10921 gatgtcctgt gacagtcgct tgtgccaggt caggatgcga tcgatcgacg cggatggatc
    10981 atccgtgaac aggagaggaa cctcttcggc gacacgttga tacccgatgc tgtctccttc
    11041 cgtctgggct tcgatgaagc cgtcggtata cagcaacaaa tggtcgccac tcaccagcaa
    11101 ttcttcagcg gttttgaccg aaatgttctg gcggctgccg agcggaaacg atggcagagt
    11161 caggttccga gcaggttctc cacgtcgaaa cagaaagggg tagttgtggc cggcattggc
    11221 atacgtcagt tgatgcgtgg tggtatcgag caggcccaga aagcaggtca tggtgcggcg
    11281 gcgcttgagg gttctgaaga gtacgagatg cagaccggcg agaagctgat cgggtgtcgg
    11341 tattgtcgat agactgcatt gatattcgat gaatgccttc gccatggcca tgaccagggc
    11401 ggctggcacg ccatgtccgg agacgtcgcc gatcagaatc aacagacggt catcggacaa
    11461 tttctgcatg tcgaaatagt ctccgccgag ctcggacgca ctcacggagc ggccgctggc
    11521 acgatacgga ccgatatgaa tttcggcagc cgggaagagg ctttcctgga cgatgcgagc
    11581 tacttccaga tcggacaatc cttccatgac acggttgaat gtcagggcca gatcaccgag
    11641 ttcattctcg cccagggaag gcacacgatg acgaaactgc cgttcctgaa tggcaacaac
    11701 cccttgggtc aacgtcgcca gcggtgtgag aaactgcctc gacagggtca agcccagtcc
    11761 caggctgaaa acaacggcaa taatcgtgaa cagagacagg cgcaggcgca ggtgcttgat
    11821 ctcgttctca attggatcag cattttcgat agcaagcaga aagaactgac tgagttcctt
    11881 gggtttgatc ccggtcagca ggtagtttcg gccgcgccac accagagttt ccgtcgtggt
    11941 cccctgctgt cgccgcaggt tggtcatgaa gttcaatagc tgttgagggc ggcttttcca
    12001 ttccggatac agtttctcgc tggcggtgtg aaaaccgaaa aaacgcgtgt ccgggcgatg
    12061 tcgattgatt ccggtcaagt gcgtctgcag aaagtaattt tccagaccga atttgtccca
    12121 ggtggctgag aacagatatt cgatgttccc tgaagctccg cgtataggaa ggaaatacac
    12181 ccacacatga aagtcggcaa aattgatggg agtgatgctg ccgaggccct ttaacaaggg
    12241 gttcaacgcg agatcgattc ccggaacact tgacacgagc atctctgaag acgtgatctg
    12301 atgcgatccc tctttgtcga tctgcttcag cactgatttg gctacgattc cgaacagctt
    12361 gcgcatctga ctgtttgccg aacgatgttc cttgcgagca tctgaacttg aacgaatacg
    12421 ccctttgacg tcgatcagaa gaaactccgc tcgaccgagt cgacgactca gacgatccag
    12481 gcgattcatg tcgacggcgt gaccgtgcag aagctgcgct tcgacctggt ggccgattct
    12541 ctggcaaaga cgctccatga tgccgcgctg cagcgggaat ctgccgtcga ggatctgaag
    12601 attcctgtcg gcgatgtcgt gtgcttcgcg aatacgggtg cgaaacttgt cgtgaagata
    12661 gtcccagccg taaaacccga gaaccatcaa gggaagcccg gaagcaaagc caaacaagag
    12721 tatcagacgg ttgcgcatgg tgaagcgaaa cgtggtctcg aggacgatga atcgataagc
    12781 cagccacgaa aagagaccga ataccaggct tccaatggcg agcaggcaga ggcgatgcca
    12841 caggaaattc ggcaggcgat cggtcggaat actcgcccag atgcgggctt tggaggcata
    12901 gggcatgacg ctgacgagat gtctcccgac ttccacatgg tcgctcaggg ttcgttcatg
    12961 ctccccaata gccagagcca gtgttccacg aagttgtgat tgacggtcga gagtgatgag
    13021 accgaacttc acttccgggt ctcgaacctg acggcgcaaa cgtctcatgc gatcacgggc
    13081 aggaatgatg tcccagtcag gtgtctggct gatatgcgcc agaagcccgc ttgagcggga
    13141 aatcagatag aaaaaccaac gattcttcgc atcggtattg gcttcgatca atcgctggcg
    13201 aaaggcgtca aatttaccga cttcggcgac gggaccgatg taaccccgta tcagaggcca
    13261 caccagttcg cgagtcgtgg aatagtctcc gcgcggagaa aactgcatct cacgaatggt
    13321 ttcgacgagt cgacgaacga tgagctttgg ggctgcggtt ggagagtatc gcggatcagg
    13381 ctcccctttt tcagtcacca tggtggtctg gaacaccccg ggaaaccttc gatgcagatt
    13441 ccagaggaca agtttgagat acgcacggga ttttccgcga gaattgtctt catcgatctt
    13501 tcgtaaacga cgcaggcagc ggtgaaagaa atcgtcgaaa aactcgacgg tgttatcgaa
    13561 cttacgaatg gtcaggagat actcgtcgag tctggaaaac agtcggtcgc gggcctcgct
    13621 gcgttgtatt tcccagaccg taccgagacc gtaggcgaca agaagaaacg gcagaccgaa
    13681 aaacatcagg caggtgacca gccagcgca
//
LOCUS       DAORHD010000003        13665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_3557_length_13665_cov_3.954445, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000003 DAORHD010000000
VERSION     DAORHD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13665
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3557_length_13665_cov_3.954445"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PKO06_00125"
     CDS             complement(<1..1220)
                     /locus_tag="PKO06_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV68069.1"
                     /translation="MSPITSAPRLSHSPDSARRAGFVLLSLFLLTVTLLQALPDRWPN
                     RTVEQIETCFADHDAAFVLRHLNSDRAIRIGGKRTAMQLSPCSTFKIPHALIGLECGI
                     LTSADQRFPWDGHSQFVKDWERDHSVRSSMYYSVVPLYKMLATQIGSEKELSWLTTLD
                     YGNRDISAGLTSFWLGTSLKISADEQIRFLTNLVSHRLPVHAPNLEVIENITRLERFS
                     DGSYHGKTGSNFRDGKWVLGWWVGWINHGNDQYVFAANVRGQGASGMIARRCAEAALQ
                     RLGLLPTTREERLTMSYQAFDQQPNGWRAVGESGTLDQGCCIEAAKLIEEYLSRHDRN
                     LENWQRRILHFHAGQMQAFAGEVTAALSHFRNSYDPIEEDRKAPLRWNAYVRATIAFL
                     QRDAATLEACRNAML"
     gene            1472..2029
                     /locus_tag="PKO06_00130"
     CDS             1472..2029
                     /locus_tag="PKO06_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68070.1"
                     /translation="MKNVVRVSAGLGLLLLALVVVQLVGCGQSGTPTPPATPAAPAAA
                     VATVPTVSALDILKSFDTDQNKAQTEFASKTIKLVNVIANTHWEDDKLLELSVYNPDT
                     KEAGGIPSETVFRGAPLKQMVCPFFFKVRIDDPKLIEGIKCSTTNTVEGKAVTELFDV
                     LEIECEFDKFVEGEIIFKNPKITKK"
     gene            2189..3625
                     /locus_tag="PKO06_00135"
     CDS             2189..3625
                     /locus_tag="PKO06_00135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HNV68071.1"
                     /translation="MKLLLEVEQGANQGKTFEFARPDVFLVGRATDAHLVINPEDTSV
                     SRRHCLVEIAPPRCYLRDLGSRNGTQVNGKSVQYTEVRHNDLIKIGRTVLRVIVCDAD
                     SPDDVCMICGRADRRAVQDSVCSDCREESVKPVQAEAAGSPAAFPVPCVDCGVDVRSI
                     ANADGLARVYQAEALYACAGCARKRFALSYEKQYGHYRLLSQLGMGGMGVVFLAQDER
                     TGRLGALKTISLPFPDELSVRRFLRETRVMAHLTHSNIVRMFRQGIDHGVPYLFTEYL
                     DGGSISDKMRSVGGKLPIDVACRWTCDMLAGLAHFHESGFVHRDIKPANILLKKSPYG
                     QTEIAKLADFGLAKSLTAAGATVLTQPNDTCGSIQYTAPEQILSYISAGPAADIYSMG
                     ISLYQMVTGVLPFAQAMKTATSKKDILLILLEESPPPVNQLNPEVPAALTAIINQAIQ
                     KTPAYRYHSAQEFQEALESFLPERSPAA"
     gene            3622..5967
                     /locus_tag="PKO06_00140"
     CDS             3622..5967
                     /locus_tag="PKO06_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV68072.1"
                     /translation="MKKCPFCAEQIQDDARKCRFCGEFLDTPVPGQVKPGDVVDQFRI
                     VRELGRGGMGTVYLAEDQSLSRQVALKVLPASLLHDPSLPKRFKKEAQVAAGLIHPNI
                     ISIFAIGDLPSGQPWYAMTFLNGGSLADRLKSGPLSVATTLHLTREILSGLGFAHSKG
                     VFHRDIKPGNILLTETGTAVIVDFGIAHASMGTEVRTIAGSMIGTPHYMSPELWNGQP
                     SDGRSDLYAMGVMLYQMLTGELPFQGGDANSIMCGHLLHPVPAASTRASGLPVALDAI
                     IERALAKDPEKRFRTAEEFLAALETETVVSGRTPVPRLNLQETTPIEATLPPHPVERP
                     ITSPGPGHVSSQTVRSVESESGSGAQPTTMGKAVRVVLGCIVVPVVALILLLILVPDP
                     EPESGSAQTDVPLATSTVSPIAATASNTAGEAGAPVRETATAERQVVQTPPLPATLTT
                     SQTTMLASPGWNTPPTTASNVATRSGWGRSLIRETGSVALNPGGLPARLAEKFPRGRL
                     YFCAEETGARQVYVASIDGQEVRQLTSEPSGVKTFRLSTAARQIAWLSVETPGRLRFL
                     HLDTGTLHEWPDDKGGRSHDITSFAWHPDGSRLIYTVFERGRQPLTRFFARSLSATAA
                     TFLASVASADCRVEDVVPGSGDIIFIKRVDMADAEDWELVRLSAQSQEQISYEEYGLS
                     PRVSPAGTEIVFARFAGADDPEAHLVVMDLEGVKARVVARAPAGSRYLEPEWVPDAGG
                     FFFVNETETGRRIWSCLSHGAEAQRLFQRQQDESLPQFLSE"
     gene            complement(6023..6535)
                     /locus_tag="PKO06_00145"
     CDS             complement(6023..6535)
                     /locus_tag="PKO06_00145"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNV68073.1"
                     /translation="MRLRLFVLIALMLVAHVAFAELPVPFERVVRDGVRYGYKDLNGT
                     VVIPAVYRLAQNFSEEGIAAVVDEKGWAIIDKTGKLLLRPFVYDNGPDYFVEGLARFR
                     ENGRLGFFNTQGQKVIPAWFDFAYPFENGVARVASGCREVRGPDEHTTIEGGVWSTID
                     LTGKQLEPWR"
     gene            complement(6537..7580)
                     /locus_tag="PKO06_00150"
     CDS             complement(6537..7580)
                     /locus_tag="PKO06_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68074.1"
                     /translation="MNILPLVQTWLLMIFLQFTPFTGQEGQQEHVFTLSGAPGVRFVI
                     DVTSPKNALNQPVLAATGHFQGNPEAVGSLTAVILGFDRLRTLVNIEDADGIDQVPAG
                     TKEQLKLFPGKDDRLAICHDGTITRVNAISEKGTDPREIEFQSDLVKALVVYDQTQGV
                     RKIRLSVDGADSLEFVAQPVKVLSTKETMKIGTEADFKHAIIREYRGLISASLTLNQG
                     RSFAMTPAKAEQIVIDNQKKYQEFVARIYPDVVTKMSPAPKSDDPLLKLPKIDFPRER
                     LIVLLAHDGMYGDMLITNLEEKGRRLQIHCRYDKPSAGETMSAQPMGVGRYRAFVLKS
                     PARKLNILVEKEQ"
     gene            7839..9113
                     /locus_tag="PKO06_00155"
     CDS             7839..9113
                     /locus_tag="PKO06_00155"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNV68075.1"
                     /translation="MSEKKKVVLAYSGGLDTSVILKWLAEKGYEVICFVGNVGQRDDF
                     NAVKEKALKTGASKVHVRDLRREFVTDFIFQALWGNAIYESRYLLGTSLARPLIARAQ
                     MEVAAEEGARYVSHGATGKGNDQVRFELTFYALDPSIKVIAPWKDAEFLAQFKGRTDM
                     IEYAEKHGIPVKAQKSKPYSEDENLMHISHEAGILEDPGFIAPDDVYSLTISPKLAPD
                     KETTIEIEFRDGTPVRVKNLDDGHEERDPLALFEYLNKVGGENGVGRVDMVENRFVGI
                     KSRGIYETPGATILWAAHRDLEAIAMDKEVMRLRDMLVPKFSEVIYNGFWFSPEMDFL
                     LAAFAKSQEALDGTVTVKLYKGNAMVVARRSPTSLYNQDLSSMEVEGGFDQTDSRGFI
                     NINAQRLKAHHLVLRQDRPFDWRRGILRRGGR"
     gene            9110..10294
                     /gene="argH"
                     /locus_tag="PKO06_00160"
     CDS             9110..10294
                     /gene="argH"
                     /locus_tag="PKO06_00160"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNV68076.1"
                     /translation="MKLWDKGYSLEAAIERFTIGDDPVLDLELLPFDCRASIAHARGL
                     HRIGLLTQSELDGIERELTQLEERARAGEISIPVGMEDCHTFIEDHLTTVLGDAGRKI
                     HAGRSRNDQVLTALRLLERDRLSLVRDAVQKVIDSLDNLDKRRGSIPIPGFTHTRKAM
                     PSSIGMWATAFRDALRDDLPLVAGVASLIDQSPLGTGAGYGVPLPLDRTFTAADLEFS
                     RVQENPIYAQLSRGKFELAIVQVLVQIMHDLNRLATDAILFSLPEFGWLKLPDRFCTG
                     SSLMPQKKNPDVLELVRAGLHVLTGAQQRLQGISGNLISGYHRDLQWTKRPLLESFAT
                     TIDSLTVMLAVIDGLEVDEARCREAMTGELYATERACDLVRQGVPFREAYRRVAEEYR
                     SK"
     gene            10337..11659
                     /locus_tag="PKO06_00165"
     CDS             10337..11659
                     /locus_tag="PKO06_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNV68077.1"
                     /translation="MGLLIKRTTAVTLNDQHAVLEDIDIRLDGTRIQELGRNLPVHPG
                     DETIDGSSLVALPAFFNAHCHAPMTFERGWAEDLPLDRWFNERIWVAESALTEEDVYW
                     GAALAACEMIRSGCAGFNDHYFYMDRVAEVVEASGMKATLTWCQFGIGEDKEVGTNLE
                     GALAFASRWRGRANGRIRTVLGPHSPYVCPPAFLKEIAQLAKQHGFGLHIHLAESSQQ
                     VVNSLKAHDRTPTELLNDCGVFDVPCVVAHALYLDDRDVEILAKRQVSVAHCPITYMK
                     LAMGVNDLTRLQTAGINIGIGTDGPGSNNDMDMKEAVRITPLLQKFHFNNPEALPGDI
                     PLRLACAGSSRALGFAESGSLEVGKSADIALFDFSGPHLQPCHDLVANLVHVAKGADV
                     RHLIVDGKLIMRDRVILTLDEEKIKSEAGRRAKAMVSKNLRVVREYQG"
     gene            complement(11691..13499)
                     /locus_tag="PKO06_00170"
     CDS             complement(11691..13499)
                     /locus_tag="PKO06_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNV68078.1"
                     /translation="MIPRQNALRISSFCRFFLLVGVLVGLCLTHTLNAETRRISTDVN
                     LPGAQPLGSGRKVALLIGNEAYGELGALSNPVNDVNAFSKALESCGFGVMTRTNCTRK
                     EMSKAVQEFADQCRGSDVALFYYSGHGSQLKGENYLWPIQADAKSEADLEDECFRAQR
                     LIDMMTEANARINIVILDACRNVPFKSGSRSSTRGLAQMNSSSGQFLIAFATDPGAVA
                     QDGVAGTNSPYMLSILNTLKKSGLSLSEFFMEVRKDVKRLTNGQQRPWETACLEDRFY
                     FNNPIPPTPEPVTTVPPAPEPVPGSSLTPTPVATPQAVPTSDTGASSASTSGAFLQFV
                     QEYGASDTGPGEFLGVVDMAITRDDDLVLLTNAAQPLQIRSSSDLSLVRSFGAIGKTG
                     FSLQTPRAVAAGTKTGNIAVCDIDSNRVVVFNREGVPVLRFVDQVNRPVDLAFDDQEN
                     LYVIMEGQPGIFKYSPFGIFMQTLSANLLPRPTRLSFAHGHLIAVDDENRKLLLYTPA
                     GKLEGEIDLTGSLQGVRDLAAVDLNGHLYLTADNDTLILADAKGKRISQVKASEVHAP
                     KFSAWNSMAITADQSIFTVDRQRGILLRFSRDAATE"
BASE COUNT         2821 a         3793 c         4195 g         2856 t
ORIGIN      
        1 agcatggcgt ttcgacaggc ctcaagggtg gccgcatcgc gctggagaaa ggcgatcgtg
       61 gcgcgaacat aggcgttcca gcgcagggga gccttacgat cctcctcaat cggatcgtac
      121 gagttgcgaa agtgcgacag agccgcggtt acttcgccgg caaaagcctg catctggccg
      181 gcatgaaagt gcaggatgcg gcgttgccag ttttccaggt tgcgatcatg gcgggaaaga
      241 tactcttcga tcagtttcgc cgcttcgata cagcatccct gatcgagagt ccccgactcg
      301 ccgacagcac gccagccgtt cggctgctgg tcgaaggcct gataactcat ggtcagtctc
      361 tcttcacgag tggtcggcag cagtccgagc cgttgcaggg ccgcttccgc gcaacgtctg
      421 gcgatcatgc cgcttgcccc ctggccgcgc acattggccg caaacacata ctggtcgttg
      481 ccgtggttga tccaccccac ccaccagcca agcacccatt ttccgtcacg aaagttcgac
      541 ccggtcttcc catgatagga accgtcggaa aatcgttcga gccgggtgat attctcgatg
      601 acctcgaggt ttggcgcatg aaccggcaaa cgatgagaaa caagatttgt cagaaacctg
      661 atctgctcgt cagctgagat tttcaaactg gtaccaagcc agaacgaagt cagtccggcc
      721 gagatgtctc tgtttccgta gtcgagtgtg gtaagccacg ataattcttt ttctgagcca
      781 atctgtgtcg ccaacatctt gtacaatggc acgacggaat agtacatgct cgaacgcacc
      841 gaatgatcgc gttcccagtc tttgacgaac tgcgagtgtc catcccaggg aaatcgctga
      901 tcggcgctgg tcaggattcc gcattcgagg ccgataagcg catgcggaat cttgaaggtc
      961 gagcacggag aaagctgcat cgccgtgcgc ttaccaccga ttcgaatcgc tcggtcgctg
     1021 ttcaggtgac gcaagacaaa agcggcatca tgatcggcaa agcaggtttc gatctgctcc
     1081 acggtacgat tgggccagcg atcgggtaac gcctgaagaa gagtgacggt cagcagaaag
     1141 agcgagagca ggacgaagcc tgctcggcgt gcggaatcgg gggagtgcga cagtcgcggt
     1201 gccgaagtga taggggacat gttgcctgta tctcctgagc gaacttctcg acctcacttt
     1261 accatagttg tccgcagagt gagcgtcatc agcgcgccag agcgagacac aaccgaaggc
     1321 tgcgcaggct gaggggagcg cacgcggctt ttccggcatg agggcggaaa aatgttgtga
     1381 aggaaccagt cgatcggtat actcggcgag cagagcccgc atcgccttgt tcgcaggcgg
     1441 atgcgcatac atctttcatc gaggagtggc tatgaagaac gtggttcgag tttcagcggg
     1501 tctgggtttg ctgctattgg cactggtcgt ggtgcaactc gtcggatgtg ggcagtcggg
     1561 cacgccgact ccgccggcga ctcctgccgc accggcagcc gcggtcgcta ccgtgccgac
     1621 ggtgtctgct ctcgacatcc tcaagtcctt cgatacggat cagaacaaag cgcagaccga
     1681 gttcgcgagc aagacgatca aactggtcaa cgtgattgcc aacacgcact gggaagatga
     1741 caagttgctg gaactttcgg tctataatcc cgacacgaag gaagccggtg ggattcccag
     1801 cgagaccgtt tttcgcggtg ctcccctgaa gcagatggtg tgcccgttct ttttcaaggt
     1861 gcgtatcgat gatccgaagc tgatcgaggg catcaagtgc tcgacgacga acacggtcga
     1921 gggaaaagcg gtaacggagt tgttcgatgt gctcgagatc gagtgcgaat tcgataagtt
     1981 cgtggaaggt gagattatct tcaagaatcc gaagatcacg aagaagtaag cctgactcaa
     2041 gaccgaacag acctcacctg gcctctcgcg gcgggtgagg tttttctttg gtcacctgtg
     2101 ggggcctgag aggtttgaca ccccgggagg tatcgggtag cctcgaacgg tcacgccgat
     2161 gagcctggaa ccaccgaggg agacgtccat gaaactgctg ctggaagtgg aacagggagc
     2221 gaaccagggt aagaccttcg agtttgcccg acccgacgtc tttctggtcg gccgcgcgac
     2281 ggatgcgcat cttgtcatca accctgagga caccagtgtt tcgcgtcgcc attgcctggt
     2341 cgaaatcgcc ccaccgcggt gttacctgcg agatctcggg tcgcgaaacg ggacacaggt
     2401 gaatggcaag agcgtccagt atacggaagt gcgtcacaat gatctgatca agatcggacg
     2461 cacggtcttg cgggtcattg tctgtgacgc ggattctccg gacgacgtgt gcatgatctg
     2521 cggtcgagcc gaccgccgtg ccgttcagga ttcggtctgc agcgattgtc gcgaagagag
     2581 cgtgaaaccg gttcaggctg aggcggcggg ctcgcccgcg gcgttcccgg ttccgtgcgt
     2641 ggactgcggc gtcgacgtgc gttcgatcgc caacgccgat gggctggccc gagtgtatca
     2701 ggccgaagcg ctgtatgcct gcgcggggtg cgcgcgaaaa cgtttcgctc tgtcgtacga
     2761 gaagcaatac gggcactatc gtttgctgag ccaattgggc atgggtggta tgggagtggt
     2821 ctttcttgcg caggacgaac ggacgggtcg cctcggagcg ctcaagacga tctctttgcc
     2881 ttttcccgat gaattgtcgg tgcggcgttt tctgcgtgaa acgagagtga tggctcatct
     2941 cacccactcg aacatcgtgc gaatgttccg acagggtatc gatcatgggg ttccatacct
     3001 gtttaccgag tatcttgacg gtggttcgat ctcggacaaa atgaggagcg tgggtggaaa
     3061 actgccgatc gacgtggcct gccgctggac ctgcgacatg ctggccggac tggcgcattt
     3121 tcacgagagc ggattcgtgc atcgcgacat caaaccggcg aacatcctgt tgaaaaagag
     3181 cccgtatggg caaacggaga ttgccaagct cgctgatttc ggcctggcca agtcgcttac
     3241 agccgcgggt gcaaccgtgc tgacgcagcc gaacgacacc tgtggctcca ttcagtatac
     3301 ggcgccggaa cagattctca gctatatcag cgccggacct gcggccgaca tttactccat
     3361 ggggatctct ctgtaccaga tggtcacggg ggtattgcct tttgctcaag caatgaagac
     3421 tgccacgagc aagaaagaca ttctgttgat tctgctcgag gagtcgcccc ctcccgtgaa
     3481 tcagctcaat cctgaggttc cggcagcact gacagccatt atcaatcagg ccattcaaaa
     3541 gactccggcc tatcgttacc attccgcgca agagtttcag gaagcgctcg agagctttct
     3601 tccggaacgt tcgccggccg catgaagaaa tgcccgttct gcgcggagca gattcaggac
     3661 gatgcccgga agtgtcgctt ctgcggtgag ttcctcgaca cgccggtgcc cggccaggtg
     3721 aagccgggag atgtggtcga tcagttccgc attgtacgcg aacttgggcg cggtggcatg
     3781 ggtacggtct atctggctga agatcagagt ctgagtcgtc aggtggcact gaaggttctg
     3841 cccgcgagct tgctgcatga cccttccctg ccgaagcgat tcaagaagga agcgcaggtt
     3901 gccgccggcc tgatacatcc gaatatcatc tcgattttcg cgattggcga ccttccgtcg
     3961 ggccagccct ggtacgcgat gactttcctg aatggcggat cactggcgga tcgtctgaaa
     4021 tccggccccc tgtctgtcgc gacgacgctg catctgacgc gggagatcct gtccgggttg
     4081 ggcttcgcgc attccaaagg cgtgttccat cgcgacatca agcccggcaa catcctgctg
     4141 accgaaaccg gaaccgcggt gatcgtcgac ttcggcatcg cgcacgcgtc aatgggcacg
     4201 gaggtcagga ccatcgctgg ttcgatgatc ggcactccgc attacatgag cccggaactg
     4261 tggaacggcc aaccatctga cggccgcagc gatctctacg cgatgggtgt catgttgtac
     4321 cagatgctga cgggcgaact tccctttcag ggcggagatg caaactcgat catgtgcgga
     4381 catctgctgc atccggtccc ggcggcgtcg acgagagcgt ccgggttgcc cgtggctctc
     4441 gatgccatca tcgagcgggc cctggccaag gatccggaaa aacgctttcg aaccgccgag
     4501 gagtttctgg ccgctctcga aaccgagaca gtggtgtcgg gccgaacgcc ggtgccacgg
     4561 ttgaatctgc aggaaacgac cccgattgag gccacgctgc cccctcaccc cgtcgaacgt
     4621 cccatcacga gccccggacc aggccacgtg tcgtctcaga ctgtcagaag cgtggagtcc
     4681 gagtcgggct caggagctca gccgacaacg atgggcaaag ccgtgcgagt cgttctgggg
     4741 tgcatcgtgg ttccggtcgt tgctctcatt ctgctactga tcctggttcc tgacccagag
     4801 cccgagtccg gttccgctca gacggatgtg ccgctggcga cctcgaccgt gagcccgatt
     4861 gcggctaccg catcgaacac cgccggcgaa gcgggggccc cggtgcgtga gaccgcgaca
     4921 gccgagcggc aggtggttca gacaccacct ctgccagcga cgctgacgac gagtcagacg
     4981 accatgcttg cgtcaccggg gtggaacacg cccccgacga cggcttcgaa tgtggcaacc
     5041 cgcagcggtt gggggagatc gctgattcgc gagaccgggt cggttgcact gaacccgggt
     5101 ggtcttcccg cacgactggc cgagaagttc cctcgtggtc gcctgtactt ttgcgcggaa
     5161 gaaacaggcg cccgccaggt gtatgtggcg agcatcgatg ggcaggaagt gcggcagttg
     5221 accagtgagc cgtccggggt gaagacgttt cgcctgtcga cggcggcacg acagatcgcc
     5281 tggctgagtg tggagacacc gggtcggttg cgttttctgc atctggacac gggaaccttg
     5341 cacgaatggc ctgatgacaa gggggggcgt tcgcacgaca tcacgtcgtt tgcctggcat
     5401 ccggacggat cgcgattgat ctacactgtc tttgaaagag ggagacagcc cttaacgcga
     5461 ttctttgccc gttcgctgtc agccacggca gcgaccttcc tcgcttcggt tgccagcgct
     5521 gactgtcgcg tggaggatgt cgttccaggc tcgggtgata tcattttcat caaacgcgtt
     5581 gatatggccg atgcggaaga ctgggagttg gtacgcttgt cggctcaatc gcaggaacag
     5641 atttcatatg aggagtatgg tttgtctcct cgcgtctcgc ccgcgggtac ggaaatcgtc
     5701 tttgcgcgtt ttgcaggggc tgatgatccc gaagcgcatc tggtcgtgat ggatctggaa
     5761 ggggtaaagg ccagggttgt ggctcgcgcc ccggctggct cgcgctatct tgagcccgag
     5821 tgggtgccag atgccggcgg ctttttcttc gtgaacgaaa ccgagacagg gcggcgcatc
     5881 tggtcctgtc tgtctcatgg cgcggaagcg cagcgattgt tccagcgaca gcaagacgag
     5941 agcctgccgc agtttctcag cgagtaggag atgcaaaaca cgcccgcctt gagactatct
     6001 caatgcgggc gtgtcgctgg gttcagcgcc agggctcgag ttgcttcccc gtgaggtcga
     6061 tggtactcca gacaccgccc tcgatggtcg tgtgctcgtc cgggccgcgc acctcgcgac
     6121 agcctgaggc gactcgggcg acgccgttct cgaatggata cgcgaagtcg aaccaggcgg
     6181 gaatgacctt ctgaccctgc gtgttgaaga agccgagacg tccgttttcg cgaaaacgag
     6241 ccagaccttc gacgaaatag tccgggccgt tgtcatacac aaacggcctg agaagcaatt
     6301 taccggtctt gtcgatgata gcccatcctt tttcatcaac cacggcggcg attccctctt
     6361 ccgagaaatt ctgtgccagg cgatacacgg ccggaataac gaccgtgccg ttgaggtcct
     6421 tgtatccata tcggaccccg tcgcgaacca cccgttcgaa cggcacggga agctcagcga
     6481 aggcgacgtg cgcaaccagc atgagagcga tcagtacgaa cagacgaagt cgcatcctac
     6541 tgctcctttt cgaccaggat gttgagtttc ctggccggcg acttcagaac gaaggcccgg
     6601 tatcgaccga ctcccatcgg ttgcgcggac atcgtttcgc cggccgatgg cttatcgtag
     6661 cggcagtgaa tctgcaaccg gcgtcccttt tcctcgaggt tggtaatcag catgtcaccg
     6721 tacatgccgt cgtgggcgag aagcacgatc aaacgctcgc gggggaagtc gatcttcgga
     6781 agtttcagaa gagggtcgtc gcttttgggg gcgggactca tcttggtgac gacatcagga
     6841 tagatgcgcg cgacgaactc ctgatacttc ttctggttgt cgatgacgat ctgttcggct
     6901 ttcgccgggg tcatggcaaa cgaacgaccc tgattgagcg tcagagacgc gctgatcagg
     6961 cctcgatact ctctgatgat cgcgtgtttg aaatctgctt cagtcccgat tttcatcgtt
     7021 tccttggtgg acaggacctt gaccggctgc gccacgaact cgaggctgtc ggcgccgtcc
     7081 acggacaggc gaatctttcg aaccccttgt gtctgatcat agacgacgag ggcttttacc
     7141 agatcgcttt gaaactcgat ctcgcgaggg tcggttccct tctcgctgat ggcgttcacc
     7201 cgggtgatcg ttccgtcgtg gcagatggcg agccgatcat ccttgccggg aaagagcttc
     7261 agttgctctt tggtgccggc gggaacctgg tcgattccgt cagcatcttc gatgttgacc
     7321 agggtgcgaa ggcggtcgaa tccgagaatg actgcggtga gtgagccgac ggcttccggg
     7381 ttcccctgaa aatgaccagt ggcagcgaga actggttggt tcagcgcgtt cttcggactg
     7441 gtgacatcaa tgacgaagcg aactccggga gcgccggaga gggtgaagac gtgttcctgc
     7501 tgcccctcct ggccggtaaa cggcgtaaac tgaagaaaaa tcatcaacaa ccaggtttgc
     7561 acgagcggaa gaatgttcac gagtgatcct ttccagaaaa gcagtagtga ctcaataccc
     7621 ccgccatgtg taacacaaat tgtatcatct gcgcatgacg tctgaaacga ggaggggacg
     7681 ctctgcttcc aaccgtcgaa agagccttga aaatcctgcg cgccccggaa atggaacctg
     7741 agttctcgca cacatcattg gctcttgact ttggtttgat gagaaccgga taatgacgcg
     7801 gcaattttcc ccaatcttca gcccttgcgg agggtctcat gagcgaaaag aaaaaagtgg
     7861 ttctggcata cagtggtggt ctcgatacgt cggtcattct caagtggctt gccgaaaagg
     7921 gctacgaggt catctgcttc gtcggcaatg tggggcagcg tgacgatttc aacgcggtca
     7981 aggaaaaggc gctcaagacg ggtgcttcca aggttcacgt gcgcgacctg cgccgtgagt
     8041 tcgtgaccga cttcattttt caggcgctgt gggggaacgc gatctatgaa agccgctatc
     8101 tgctcggcac ctcgctggcc cgtccgctga ttgcgcgggc gcagatggaa gtcgccgcgg
     8161 aagagggggc ccgctacgtg tcgcacggcg cgaccggcaa aggcaacgac caggtgcgct
     8221 tcgaattgac tttctacgct ctcgatccca gtatcaaggt gatcgcgccc tggaaggatg
     8281 cggaattcct ggcccagttc aagggtcgca ccgacatgat cgagtatgcg gaaaagcatg
     8341 ggattccggt gaaggcgcag aagagcaagc cgtacagcga agatgaaaac ctgatgcaca
     8401 tcagtcacga agcggggatt ctcgaagacc cgggtttcat cgcccccgac gatgtctaca
     8461 gcctgacgat ctcgccgaag ctggctccgg acaaggagac cacgattgag atcgagtttc
     8521 gcgacgggac gccggtacga gtcaagaatc tcgatgacgg gcatgaagag cgtgatccgc
     8581 tggcgctgtt cgaatatctg aataaagtcg gtggggaaaa cggggtcggt cgcgtcgaca
     8641 tggtcgagaa ccgcttcgtc ggcatcaagt cacgtggtat ctacgaaacc cccggggcga
     8701 ccattctctg ggcggcgcat cgcgatctgg aagccatcgc catggacaag gaagtgatgc
     8761 gactgcgcga catgctggtg ccgaagtttt ccgaagtcat ctacaacgga ttctggttct
     8821 cgcccgaaat ggactttctg ctggcggcgt tcgccaagag ccaggaagcg ctcgatggaa
     8881 cggtgaccgt gaaattgtac aagggcaatg cgatggtcgt ggcgcgccgc tccccgactt
     8941 cgctctacaa tcaggatctc tcgagcatgg aagtcgaagg tggctttgat cagaccgact
     9001 cgcgcggctt catcaacatc aatgcgcagc ggctcaaggc gcatcatctc gtgttgcgtc
     9061 aggaccgccc cttcgactgg cggcgcggca ttctgcggcg cggcggccga tgaagctctg
     9121 ggacaaaggc tacagtctcg aagcggcgat cgaacggttc accatcgggg acgacccggt
     9181 gctcgacctc gagttgttgc cgttcgattg ccgcgcgtcg atcgcgcatg ctcgtggcct
     9241 gcatcgcatc ggcctgctga cgcagagcga actggacgga atcgaacgcg agttgacgca
     9301 gctcgaggaa cgggctcgtg ccggtgagat ctcgattccg gtgggaatgg aagactgcca
     9361 caccttcatc gaggatcatc tgaccacggt gctcggcgac gccggccgga agattcatgc
     9421 cgggcgttcg cgcaacgacc aggtcctcac ggctctgcgt ctgcttgaac gtgaccgtct
     9481 gtcgctggtc agagatgctg tgcagaaggt catcgattcg ctcgacaacc tggacaaacg
     9541 acgtggttcg ataccgattc cggggttcac ccacacgcgt aaagccatgc cttcctcgat
     9601 cgggatgtgg gcgacggcgt ttcgtgatgc cttgcgcgat gacctgccgt tggttgccgg
     9661 ggtggcgtct ctgatcgatc agtccccgct ggggacgggc gccggatacg gggttccgtt
     9721 gccgctcgat cggacgttca cggcggccga tctggagttc tcgcgtgtgc aggaaaatcc
     9781 gatctacgcc cagttgtcgc gggggaagtt cgagctggcg attgtccagg ttcttgttca
     9841 gatcatgcat gatttgaacc gcctggcgac ggatgccatt ctgttctctc tgcccgagtt
     9901 cggctggctg aaactgcctg accgattttg tacgggcagt tcgctgatgc cgcagaagaa
     9961 gaacccggac gttctcgaac tggtcagggc cgggttgcat gttctgaccg gagcgcagca
    10021 gcgactgcag ggcatcagcg gcaatctgat cagcgggtat caccgtgacc tgcaatggac
    10081 caaacgaccg ctgttggaat cgttcgccac cacgatcgat tcgctgaccg tcatgctggc
    10141 cgtcatcgac gggctcgagg tcgacgaggc gcgctgccgc gaagccatga ccggtgaact
    10201 gtatgccact gaacgcgcct gcgatctggt gcggcagggg gttccgttcc gcgaggccta
    10261 tcgccgcgtc gcggaagagt accggtcgaa gtagagcgaa agacgcggtg gcgaagagaa
    10321 aggatggggg tggaatatgg gactgctgat caaacgaacg acggccgtca ctctgaacga
    10381 tcagcacgcg gttctggaag acatcgacat tcgtctcgac ggtacccgca ttcaggaact
    10441 tggcaggaac ctgcccgtgc acccgggcga tgaaaccatt gacggttcga gtctcgtggc
    10501 tttgccagca ttcttcaatg cgcactgtca cgcaccgatg accttcgaac gcggctgggc
    10561 cgaagatctg ccgcttgatc gctggttcaa cgaacgaatc tgggttgccg agtcggcact
    10621 cacagaagaa gatgtatact ggggagcggc tctggccgcg tgcgagatga ttcggtccgg
    10681 gtgtgccggg ttcaacgatc attacttcta catggatcgc gtggccgagg tcgtcgaagc
    10741 gtcaggaatg aaggcgaccc tgacctggtg ccaattcggc atcggcgagg acaaagaagt
    10801 gggaacgaac ctcgagggag cacttgcctt cgcgagccgt tggcgcggtc gggcgaacgg
    10861 gcgcatcaga acggtgctgg ggccgcattc tccgtatgtc tgtccgccgg cgtttctgaa
    10921 agagatcgct cagcttgcga aacagcacgg attcggtctg cacattcacc tggccgagtc
    10981 gtcacagcag gtggtgaatt cgctgaaagc gcatgatcgc actccgaccg aactgctgaa
    11041 cgactgtggt gttttcgacg tgccgtgcgt ggtggcgcat gctctgtatc tcgatgaccg
    11101 tgacgtcgag attctggcga agagacaggt gtcggtggcg cactgcccga tcacctacat
    11161 gaagctggcg atgggcgtga acgatctgac gcgcctgcag acggccggaa tcaatatcgg
    11221 cattggtacg gacggaccgg gttcaaacaa cgacatggac atgaaggagg ctgtgcggat
    11281 cacccctctg ttgcagaagt tccacttcaa taatcccgaa gcgctccccg gagacattcc
    11341 gttgcgcctg gcctgtgcgg gcagcagtcg agcgctcggc tttgccgaat caggcagtct
    11401 cgaggtgggg aagtccgcgg atatcgcgct gttcgacttt tccggcccgc atctgcagcc
    11461 ctgtcacgat ctcgtcgcca atctggtgca cgtagccaag ggcgcggacg tgcgtcacct
    11521 gatcgttgat ggaaagctga tcatgcgcga ccgggtcatt ctgaccctcg acgaagagaa
    11581 gatcaagagc gaggccggcc gccgggccaa agccatggtc tccaagaacc tgcgcgtcgt
    11641 ccgcgaatac cagggctgac tctgctttcg ttctcctgtt tggttgcact ctattccgtc
    11701 gcggcatccc tggaaaaacg cagaagtatc ccacgctgtc gatcaacggt gaagatggac
    11761 tgatcggcgg tgatggccat gctattccat gcggaaaact tcggagcgtg tacttccgaa
    11821 gctttgactt gagagatcct cttgcctttt gcgtctgcca agatcagcgt atcgttgtca
    11881 gccgtcaggt acaaatgtcc gttgagatcg acggcggcga gatctcggac accctggagt
    11941 gaacccgtca aatcgatttc gccttccagc ttacccgcgg gtgtgtagag aagaagcttg
    12001 cgattctcgt catccaccgc gatcaggtgt ccatgagcaa aggacagcct tgtcggtcga
    12061 ggcagaaggt tggccgaaag cgtctgcatg aaaatcccga agggagaata cttgaagatc
    12121 cctggttggc cttccatgat cacgtagagg ttctcctggt cgtcgaatgc gagatcaact
    12181 gggcggttga cctggtccac gaaacgcaga acgggtaccc cctcgcggtt aaataccacc
    12241 actctgttgc tgtcgatgtc acatacggca atgttgccgg tcttggtgcc agcagcgaca
    12301 gcacgaggtg tttgcagcga aaaaccggtc ttgccgattg cgccgaaact tcgcaccaga
    12361 gacaggtcgg aactcgaacg tatctgcaat ggttgagccg cattggtgag aaggacgagg
    12421 tcgtcgtccc tcgtgatcgc catatccacg acccccaaaa actctcccgg tcctgtgtct
    12481 gatgctccgt attcttgtac gaattgcaga aatgccccgg aggtggacgc ggacgacgca
    12541 ccggtgtcac ttgtcggaac agcctgagga gtggccaccg gtgtcggcgt cagggaactg
    12601 cccggcacgg gttcaggtgc tggaggaact gttgttactg gttcgggagt gggagggatg
    12661 gggttgttga aatagaatcg gtcctcgagg caggcggttt cccaggggcg ctgctggcca
    12721 ttggtcagtc gcttgacgtc cttgcgcact tccatgaaaa actcggacag gctcaacccg
    12781 gatttcttga gggtgttcaa aatggataac atgtacggag agttggttcc cgccacgccg
    12841 tcctgggcga ccgctccggg atcagtcgca aaggcaatca ggaactgccc actggaggag
    12901 ttcatctgag ccagaccacg ggtactcgac cgagaacccg atttaaacgg cacgtttcga
    12961 caggcatcca gaatcacgat attgatgcgc gcatttgctt ccgtcatcat atcgatcagg
    13021 cgttgggcgc gaaagcactc gtcttcaaga tcggcttcgc tcttggcgtc agcctggatc
    13081 ggccagaggt agttttcccc cttcaactgg ctgccgtgac cggagtaata aaacagtgcg
    13141 acatcacttc cgcggcattg atctgcaaat tcctgaacgg ctttactcat ttcttttctt
    13201 gtacaatttg ttctcgtcat gaccccaaat ccacatgact cgagggcttt cgaaaaggcg
    13261 ttgacatcat tgaccggatt gctcagtgcg cccagttcgc cgtaagcctc gtttccgatc
    13321 agcagggcga cctttctgcc actgccgagg ggttgagcac ctggcagatt gacgtctgtc
    13381 gatattcttc gcgtttctgc gttcagcgtg tgtgtcaggc acaaaccgac gagcacaccg
    13441 accagcagaa agaatctgca gaaagaagaa attcgcaacg cgttctgacg cggaatcatg
    13501 gcagtcctcc aaagaactcg atgtctcgtc atactagagg aataaccgac aagcttcaag
    13561 ggaagactca tgggcaggcg cgtttcacac tcgccagaga tgactgccaa cgcaaagtcg
    13621 acggagtgcg aggactctga ctctggtgtg gattgaccat cgggg
//
LOCUS       DAORHD010000004        12841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_3969_length_12841_cov_4.159315, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000004 DAORHD010000000
VERSION     DAORHD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12841
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3969_length_12841_cov_4.159315"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="PKO06_00175"
     CDS             <1..530
                     /locus_tag="PKO06_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68079.1"
                     /translation="KASTDLAGTVVQLFRRGKDEAVSLSQDAAIKFALTDLEKEATEL
                     AKEAGRVLFDLYQRGDLKYPALLTIGHEILRMNFFIQKYTDEEAKTPAKSGGFFSQIK
                     SLFSKSQPKDPKQSQVERFTQNREDLFLRMGRLAFLKMEQKEFESSDLEGYFQTYTNL
                     QAKIAREKDKMNAPA"
     gene            555..1067
                     /locus_tag="PKO06_00180"
     CDS             555..1067
                     /locus_tag="PKO06_00180"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNV68080.1"
                     /translation="MDLNAYIRDVPDFPKKGILFKDITPLLGDAKAFDAAIEGLAAKY
                     RGQGISAIAGIESRGFILGAALALKLNVGFIPIRKPGKLPYKTKKREYSLEYGKDAIE
                     IHEDAVKPGQKVLLVDDVIATGGTARAAADLLKEMGANLVGIAFLIELSFLKGAERLK
                     GEPVFSLLRF"
     gene            1077..2066
                     /locus_tag="PKO06_00185"
     CDS             1077..2066
                     /locus_tag="PKO06_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68081.1"
                     /translation="MPAKTTTPRKPFPDLNHKEWEKRFVCAKRLSTPAGQGEFRKLAA
                     ASRREVTKRLVDLLNDDDSRVRMQTAAALGNLGCRDAAEALITALADPNEWVRVQVVE
                     ALGRIGTEDLASILAHHLEGESEAHVRATLIKVLGMIGSDKLVPVLALYLEDRDARVR
                     ANCVEAMLRLNSSKDAMRRQLLKLTSDVNNRVLANTALGLVSLGERRGRAILVRMLES
                     ADEYMRASATYALGEIRDRHDLSTLVRLLGDNSWMVRQNAVRALVKFGRTILEVMRES
                     LRSTNPAVRLGALDVCGHLGGATLRQSVIELLEDDSGEVRSKAEEVLDRLAGH"
     gene            2078..2806
                     /locus_tag="PKO06_00190"
     CDS             2078..2806
                     /locus_tag="PKO06_00190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HNV68082.1"
                     /translation="MSDALAVSRLGVILARAGSKRLPGKNVRPFAGRPLIAWVIQAAL
                     SSEVFDRVLVSTDCSAIAREARAWGAWVPFLRPPELATDQASSLDALVHAVRWVVDQQ
                     PTDTSLQLVGLLQPTSPFLTATHVREAVDLFDRQSCHSLSSMCPIHERPEWMFHRTPS
                     GLATPVDPEGVTKSSRDLPPLYRENGALYLLLPELLLKHHRLYDFEHHGSYLMSAVDS
                     IDIDDASDWEIASAVMSARCQGHL"
     gene            2803..3867
                     /locus_tag="PKO06_00195"
     CDS             2803..3867
                     /locus_tag="PKO06_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNV68083.1"
                     /translation="MKPFHFGSRRIGTGFPCFIIAEAGVNHDGDLEAARRLVRLAREV
                     GADAVKFQTFQTHRLVARGTAMAEYQKQNLAIDADQAAMLKALELGASEFRELFALGR
                     EIGIPVFSTPDEEFSARLLAELDPPAFKIGSGEVTNLPFLRLIGGLGKPVILSTGMST
                     LAETANAVEALEAGGATEIAILHCVSSYPAPVESLNLRVLQTLAQAFPYPIGFSDHSL
                     GFEASLGAVALGATLLEKHFTLDRSLPGPDHSSSLDPQQFSQYVRQIRNLEAALGNGV
                     KQPAAIELATRAVVRKVLVATRDLKSGTTLAEPDLLAKRSDNQAGVQPADLEKVVGMA
                     LARDLSEDEALTWDHLRPQR"
     gene            3952..4533
                     /locus_tag="PKO06_00200"
     CDS             3952..4533
                     /locus_tag="PKO06_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNV68084.1"
                     /translation="MKVVAFNGSPREKGNTALSLGLVTAELEKEGIQTETVSLSGQNL
                     RGCVACGKCFENKNRRCVIEADPMNDYMTKMFEADGVLLGSPVYYADITANLKALIER
                     TGYVAGANGGLLARKVGAGVIAVRRGGAIHSFDSINHFFLISQMIIPGSRYWNMTIGR
                     DPGEVLNDQEGVKTMEILGQNMAWLLKKIKPSG"
     gene            complement(4640..6130)
                     /locus_tag="PKO06_00205"
     CDS             complement(4640..6130)
                     /locus_tag="PKO06_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016944530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV68085.1"
                     /translation="MSNAHLEKLHAIRKAAGKPLTVGLDDQTILDFLARDPDLGRAID
                     MAVTAREQLLKEHAEHFKMEEKALCKLVQEKLLNFYPGPGINPYVPLAAAGSWIVTTH
                     GAVVHDSGGYGMLGMGHAPQKVLEAMAKPFVMANVMTPSFSQKHLTDRLQKEIGRVRG
                     TCPFDKFVFLNSGSESVTFTCRIVDIHGRKMTDAGKPHAGKKINRIAIVEGFHGRTEG
                     PARLSQSCRKAYATHLASFRDPDNMIFLPVNDVPALQQAYEKAAKDGIFIDAVFVEPV
                     MGEGVPGLALSREYYDTARSLSKKYGSLLIVDSIQAALRAQGCLSIVDYQGYENCEPP
                     DMETYSKALNAGQYPLSVTALRKEVADQYVTGLYGNTMTTNPRACEVACAVLDSVTEQ
                     VRTNIRERGKQFITGFEKLAKEFPGAIDRVVGTGLMACALLNPDKYRVTGEGGFEEWL
                     RRNGIEMIHGGDFGLRFTPSFSITKEEVDLIMRVIARGLKELAGKK"
     gene            complement(6309..6584)
                     /locus_tag="PKO06_00210"
     CDS             complement(6309..6584)
                     /locus_tag="PKO06_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNV68086.1"
                     /translation="MLLMQLLQYFQIFVLVRVMMTWIMPGPQEPWVKKVFYPVDVFLK
                     PFRTVIAFGGLMFDVGPVLALLFLHVLALVLSHFVLPPQLVNNLLLR"
     gene            complement(6596..7309)
                     /locus_tag="PKO06_00215"
     CDS             complement(6596..7309)
                     /locus_tag="PKO06_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNV68087.1"
                     /translation="MIDITRNLADVKTRIAQAAQRAGRSPETVKLVAITKTVGVSEIR
                     QLIAAGQTTFGENRPQALRDKVTELTGVKSIHWHLVGTLQSNKIKYVYPVAEMIHSID
                     RRDLFEDLARWTAKSGRACPLLIEVHISPEDTKHGFAPEEILPLLRELRSRDDLNVQG
                     LMGMAPFTDDLEMIRRCFRGLAELFQRSRELEGPGYHALELSMGMTDDFEIAIEEGAT
                     LVRIGRALFVDEHGNDPSA"
     gene            complement(7306..9078)
                     /locus_tag="PKO06_00220"
     CDS             complement(7306..9078)
                     /locus_tag="PKO06_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68088.1"
                     /translation="MKKPFSFSTTSLPVLITVLFFLFLLGGLLIRVYQADRDLGNLRL
                     AEEQARLAAEAGLHFGVVRMRERLNQPGGAMHPSYFSWLHDLADPPKLWRALSAGSGA
                     SFRLNETRRLPGFDREETPLIDEAHLFRLVSEGRVRNQIGKATGIVAATPLVKRFAVF
                     NSLNEYYYGQPIQPWVRLAGGLSAFMEKNSELFVRGALTSLGVCHDPGLLVKMFQPGG
                     ADPFAPIAGQTVLRGNYGTVHHKRGGASPCEGPLYCQTPIVVDSHVFKGPVQTASFFY
                     HRGESKPTLEHDSVALALSNSKRIQSVLDKAEGDLPENGVFVDEMTTPRTAFMASWRP
                     NFQALREYAKTSGIYIDASGQGFCRGEPTQVSYHVGSQSTYSETYFSPTSVTPEQDVV
                     DESFIVLSTATKFDGVNNLDRASLKGVNIVFSELSVMMRGEVEGDVMIVTPRHIYITG
                     SINPDSPTSLFLVAGDGTAFHTRDLEEYFLKNPPSEDLRDAARQWIVRAALYKPGAGW
                     YGAWSRKAQGDELLVPRNLLSGAQVSLTIHGACIEGSLSRWIEHAAPEGIHIKWVPGI
                     CDRLPVEPMSVNLVRTRLLSGDQP"
     gene            complement(9075..9608)
                     /locus_tag="PKO06_00225"
     CDS             complement(9075..9608)
                     /locus_tag="PKO06_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68089.1"
                     /translation="MKNTRRAYKRQQTTQLRLLLTLLAVGTASLVFFSFLSSRDMKSA
                     QFRSSWARQVETVLDRLSQELENAAVVEFPSQGEGPQCAFRRATADWTLGAAVEIDSF
                     LFAENSLYHMIRTASGTTPAPRPIGNLTNPLISGVQGGVFERVAPRLLKLRCRVQLPD
                     KPEIFQPFERLIHLRNQ"
     gene            complement(9605..10012)
                     /locus_tag="PKO06_00230"
     CDS             complement(9605..10012)
                     /locus_tag="PKO06_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68090.1"
                     /translation="MNDINGKGRPEARAGRSTVDWLLIFGILTFLVWPFISLWQIQAF
                     PAWDSETYQRAAVAVKNLMAEARSQPFRDAFQEKGFRTLVTTGDHEIEGRVEFLPHPE
                     LPGMTLIRAQVRWGYPLFKKSLFLESTITQTRP"
     gene            complement(10039..11106)
                     /locus_tag="PKO06_00235"
     CDS             complement(10039..11106)
                     /locus_tag="PKO06_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV68091.1"
                     /translation="MKRFIYVLVTVFSLGAGVSEAAIYSYRDESGRLFLTDNPPNSKY
                     QIVVPTRKDRAGPDLPTSWNGNSSTMVTEMAPQKILLPNDETYSEIINEAANYQGVDP
                     YLIKAVIKVESDFDPNVVSSKGACGLMQLIPGTAKLVKCQNSFDPRDNIHGGARYLRM
                     MLDRFQGNVDYALAGYNAGPGRVEQHGGIPPIRETKNYVKKVNHYYKLFRSGVPAAPA
                     MTRTVRAIPVVPSTLSKKLNAAYAMYLDNNVDGAIRSYQDILNIYPRNTQALYNLACL
                     LDMERRYDEAIEAYQASVKQDPYLDKSLYNLAIIYERLGQHREAIACWKRYTVTVKDE
                     DKIAMAERYVRELEEYAALQP"
     gene            complement(11259..11552)
                     /locus_tag="PKO06_00240"
     CDS             complement(11259..11552)
                     /locus_tag="PKO06_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68092.1"
                     /translation="MANPLPVPPAVALSGNVSPNPVPVSPPPNLTVVPTPSPDPKPID
                     LLDLYKTQQRECWMCKKAAADLEAGKTLDEEYAWHFVTCVLSWAHLNPAPVEA"
     gene            complement(11790..12005)
                     /locus_tag="PKO06_00245"
     CDS             complement(11790..12005)
                     /locus_tag="PKO06_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68093.1"
                     /translation="MIDLRKFLEDNKRLFDPNDGVEKEKARIVASRNELLAKARGSLR
                     QHLQQQLSEALRRFQEEEVPSDPRQLS"
     gene            12130..12450
                     /locus_tag="PKO06_00250"
     CDS             12130..12450
                     /locus_tag="PKO06_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNV68094.1"
                     /translation="MWTFAAHSDALGLNRSLVVTVQHQRLILVRTTAGVFALDDRCPH
                     QEQTFADGDVEEHALCCPWHSVKVDLRNGKVLNDMGFRGLRDVPVFAVKEEAGQILVE
                     LPAS"
     gene            complement(12559..>12841)
                     /locus_tag="PKO06_00255"
     CDS             complement(12559..>12841)
                     /locus_tag="PKO06_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68095.1"
                     /translation="NDRAPPQRLPPLVFCHRKIAMIPPRVNVETVQAAIGEYADPVAS
                     REERGQIYQELIGFVPPRIEARLNVTGALDPKMLDLQEQLLSAVRQEDR"
BASE COUNT         2744 a         3819 c         3601 g         2677 t
ORIGIN      
        1 gcaaagcgtc gactgatctt gctggtactg tcgtgcaact gttcagacgc ggcaaagatg
       61 aagcggtcag cctgtcgcag gatgccgcga tcaagttcgc gctcaccgat ctcgagaagg
      121 aagcaacgga actcgccaag gaggccggtc gcgttttgtt cgatctgtac cagcgcggcg
      181 acctgaaata cccggctctg ctgacgatcg gtcacgagat tctgcgcatg aacttcttca
      241 ttcagaaata caccgacgaa gaagcgaaaa ctcctgccaa atcaggtggt ttcttttcgc
      301 agatcaagag cctgttttcc aagtcgcagc cgaaagatcc gaaacagtcg caggtcgaac
      361 ggttcacgca gaaccgcgaa gacctctttc tgcgcatggg gcgactcgcc tttctgaaaa
      421 tggaacagaa agagtttgag tcttctgacc tcgaagggta tttccagacg tatacgaacc
      481 tgcaggcgaa gatcgcccgc gagaaagaca agatgaacgc cccggcctga ccgggaacca
      541 cgggaggcaa ccgcatggat ctcaacgcgt acattcgcga cgttccggat tttccgaaaa
      601 agggtattct gttcaaggac atcactcctc tgctcggcga tgccaaggct ttcgacgcag
      661 ccatcgaagg gctggcggca aagtatcgcg gacagggtat ttcggccatc gcgggcattg
      721 agtcgcgcgg gttcattctg ggcgcggctc tcgcgctcaa actcaacgtc ggcttcattc
      781 cgatccgcaa accgggcaag cttccgtaca aaacaaagaa gcgcgaatac agcctggaat
      841 acgggaaaga tgccatcgaa attcacgaag acgccgtgaa gcccggccag aaagtgttgt
      901 tggtcgatga cgtcatcgcg acaggcggca ccgctcgcgc agccgcggac ctcttgaaag
      961 agatgggcgc gaacctcgtc ggtattgcgt ttctcatcga actgtccttt ctcaaggggg
     1021 ccgagcggct gaagggcgaa cccgttttca gtctgctgcg gttctgaggc cacaacatgc
     1081 cagccaaaac cactaccccc cgcaaaccct ttcctgacct gaaccacaaa gagtgggaaa
     1141 aacgcttcgt gtgcgcgaag cgcttgtcga caccagccgg acagggcgag ttccgcaagc
     1201 tcgccgccgc cagccgacgc gaggtcacca aacgcctggt cgacctgctc aacgacgacg
     1261 actcgcgtgt tcgcatgcag actgccgccg ccctggggaa tctcgggtgt cgtgacgctg
     1321 ccgaagccct gatcaccgcg ctggccgatc cgaacgaatg ggtgcgggtg caggtggtcg
     1381 aagccctcgg gcgcatcggc accgaggatc tcgcctcgat tctggcccat cacctcgaag
     1441 gagagagtga agcgcacgtt cgtgccacct tgatcaaggt tctgggtatg atcggctcgg
     1501 acaaactggt tccagttctg gccctgtatc tcgaagaccg cgatgcccgc gtgcgcgcca
     1561 actgcgtcga ggccatgctg cgcctgaaca gttcaaaaga cgccatgcgc cggcagttgc
     1621 tcaagctgac ctctgatgtg aacaaccgcg ttctggccaa caccgcgctc ggactggtgt
     1681 cactgggcga acgacgcggc agggcgattc tggtgcgtat gctcgaatcg gccgacgagt
     1741 acatgcgggc ttcggcaacg tatgccctcg gggagattcg tgaccgtcac gatctctcga
     1801 cgctggtgcg cctgctgggt gacaactcct ggatggtgcg ccagaatgcg gtacgcgctc
     1861 tggtcaaatt tggtcgcacc attctcgagg tcatgcgcga atcactgcga tcgaccaacc
     1921 cggccgttcg acttggcgca ctcgacgtct gcggccacct tggtggggca acactgcgcc
     1981 agtccgtgat cgaactgctc gaggacgatt ccggcgaagt gcgatcgaaa gccgaagaag
     2041 tgctcgatcg actcgcagga cactgaattc cccccgtatg agcgatgctc ttgccgtttc
     2101 acggctcggg gtcattctgg cgcgcgccgg ttccaagcgt ttgcccggca agaatgtccg
     2161 cccgttcgcc ggacgccccc tcatcgcctg ggtgattcag gccgcgctgt catcagaagt
     2221 cttcgatcgc gtcctcgtct cgactgactg ttcggccatc gcccgcgagg cccgagcctg
     2281 gggcgcctgg gttccgtttc tccgcccacc ggaactggct actgatcagg cttcttcgct
     2341 cgacgcgctc gtacacgcag tgcgctgggt ggttgatcaa caacccaccg acacgtcgct
     2401 gcaactcgtc gggctcctgc agcccacctc gccgtttctc acggcgacgc atgtgcgcga
     2461 agcggtggac ctgttcgacc gtcaatcgtg ccactcgctg agttcgatgt gcccgattca
     2521 tgaacgtcct gagtggatgt tccatcgcac cccctcagga ctggccactc cggtagaccc
     2581 cgagggagtc accaagtcgt cgcgcgacct gccccccctc taccgggaaa acggcgcgct
     2641 ctatcttttg cttcccgagc tgcttctcaa acatcaccgc ctgtacgact tcgaacatca
     2701 cggttcgtat ctgatgtcgg ccgtcgactc gatcgatatc gatgacgcct cagactggga
     2761 aatcgccagc gcggtcatga gcgctcggtg tcaggggcac ctgtgaaacc gtttcatttc
     2821 ggcagtcgcc gcatcggcac cggctttccc tgtttcatca tcgccgaagc cggggtcaac
     2881 catgacggcg atctcgaagc cgcgcgccga ctggtgaggc tggcacgcga agtcggtgcc
     2941 gacgcggtga agtttcaaac ctttcagact catcgactcg tggcgcgcgg caccgcaatg
     3001 gccgagtatc aaaagcagaa tctcgcgatt gatgccgatc aagccgccat gctgaaggct
     3061 ctcgagcttg gtgcgtcgga gtttcgcgag ctgttcgcac tcggtcgcga gatcggtatt
     3121 ccggtgttct cgactcccga cgaagagttc agcgctcgtc tgctggccga gctcgatccg
     3181 ccggccttca agatcgggtc gggcgaagtc accaatctgc cgtttctgcg attgatcggc
     3241 ggcctcggca aacccgtgat tctttcgacc ggcatgtcga cgctggctga aaccgcgaat
     3301 gccgtcgaag ccctcgaagc cggaggcgca accgagatcg ccattctgca ttgtgtttcg
     3361 tcctacccgg ctccggtcga gagcctgaat ttgagggtat tgcagaccct ggctcaagcg
     3421 ttcccatatc cgatcgggtt ttccgatcac tcgctgggat tcgaagcctc tcttggggct
     3481 gtcgctctcg gcgccaccct gctggaaaag cacttcacgc tcgaccgctc gcttcccggg
     3541 cccgatcatt ccagctcact cgatccgcag cagttttcgc agtatgtgcg tcagattcgc
     3601 aacctcgaag ccgcgctggg gaacggggtc aagcaaccgg ccgccatcga gctggccacg
     3661 cgagccgtcg tacgcaaagt gctggtggcc acacgggatc tcaagagcgg aacaacgctt
     3721 gccgagcccg acctgctggc caaacgttcg gacaaccagg caggcgtaca acctgccgat
     3781 cttgagaagg tcgttgggat ggctttagcc agagatctat ccgaagacga agccctgacc
     3841 tgggaccacc ttcgtccgca gcggtagtct cttcgccagt ccgtggtaac atacgcgagg
     3901 ccgttttggc ctgcgcatct ttcgaagccg acaaaggaga ctcatctcat gatcaaagtc
     3961 gtcgcgttca atggcagccc gcgtgaaaag ggcaacaccg ctctttccct cggcctcgtc
     4021 accgccgaac tcgaaaaaga gggaattcag accgagacgg tctcactgtc cgggcagaat
     4081 ctgcgaggct gtgtcgcctg cggcaagtgc ttcgagaaca agaaccgccg ctgcgtcatt
     4141 gaggctgacc cgatgaatga ctacatgacc aagatgttcg aagcggatgg cgttctgctc
     4201 ggctcgcccg tctattacgc cgacatcacc gccaacctca aagcgttgat cgagcgtacc
     4261 ggttacgtcg cgggtgccaa cggtggcctg ctggcgcgaa aggtcggtgc cggcgttatc
     4321 gccgtacgcc gcggtggcgc gattcactca ttcgattcca tcaatcactt cttcctgatc
     4381 agtcagatga tcattcccgg ctcgcgctac tggaacatga ccatcggtcg cgacccgggt
     4441 gaagtgctca acgaccagga aggtgtgaag accatggaaa ttctgggtca gaacatggcc
     4501 tggctgctca agaagatcaa accctccggc tgagggtaaa ggacggcgag atcagagaac
     4561 gtcgccgcta agaaaaacgc ctcgttccaa ctgttggaac gaggcgtttt attcagagct
     4621 ctcgaatgag ggagtcgagt tacttctttc cggccagttc tttcaggccg cgtgcgatca
     4681 cgcgcatgat cagatcgact tcctctttgg tgatcgagaa cgatggagta aaccgcagac
     4741 caaagtcgcc accgtgaatc atctcgatgc cgttccggcg caaccactcc tcgaacccgc
     4801 cttcaccggt gacccggtat ttgtcggggt tcaacagcgc gcacgccatg agaccggtgc
     4861 cgaccacacg gtctatcgct ccggggaatt ccttggccag cttctcaaaa ccggtgatga
     4921 actgtttgcc acgctcgcga atattagtgc gtacctgttc ggtcacgctg tcaagcacag
     4981 cacaagccac ttcacaggca cgcggattgg ttgtcatggt gttgccatac agaccggtga
     5041 catactgatc ggcgacttcc ttgcgcaacg cagtgactga cagaggatac tgaccggcat
     5101 tgagcgcctt cgagtaggtt tccatgtcag gcggctcgca gttttcatac ccctgatagt
     5161 cgacgatgct taaacatcct tgtgctcgca acgctgcctg gatcgaatcc acaatcagca
     5221 gcgacccgta ctttttcgac aaagaccggg cggtatcata gtattcgcgg ctcagcgcca
     5281 gacccggcac gccttctccc atgaccggct cgacgaacac ggcatcgatg aaaatgccgt
     5341 ctttggcggc cttctcatac gcctgctgca acgccggtac gtcattcacc ggcagaaaga
     5401 tcatgttgtc cgggtcacga aatgacgcca ggtgggtggc atatgccttc cggcacgatt
     5461 gcgacaaccg ggccggtcct tcggtgcgac catgaaatcc ttcgacgatc gcgatgcgat
     5521 tgatcttctt gccggcatgc ggcttcccgg catcggtcat cttgcggcca tggatatcga
     5581 cgatgcgaca ggtaaaagtc actgattctg aaccgctgtt cagaaagacg aatttgtcga
     5641 agggacaggt gccgcgaacg cgaccgatct ctttctgcag ccgatcggtg aggtgtttct
     5701 ggctgaacga cggggtcatg acattcgcca tgacgaacgg cttggccatc gcctcgagca
     5761 ccttctgcgg cgcgtggccc atgcccagca tgccgtagcc gcccgaatca tgcacgacag
     5821 caccatgcgt ggtcacgatc caggatccgg ccgccgccag cggcacatac gggttgatcc
     5881 ccgggcccgg atagaagttg agcagttttt cctgcacgag cttgcagagc gctttctctt
     5941 ccatcttgaa atgctcggca tgttctttga gcagctgttc gcgggcagtg acggccatat
     6001 cgatagcgcg ccccagatcc gggtcgcgtg caagaaaatc gagaatcgtc tggtcatcga
     6061 gaccgacggt cagaggcttg cctgcggctt tgcgaatggc atgaagtttt tcgagatgtg
     6121 cgttgctcat cattccctcc ttggcctgct gccggttgtt ttcatgatcg agacggtcat
     6181 ggccggtcac gcagacgcag ggaaaagtgt atccgacctt ggcttttcag gcaacccaca
     6241 cagcagaaac aaccggccac accgattcat gaccggcctc atgccgaatc gagcgggtga
     6301 gaagggggct atctcaggag aagattgttg accaactgcg gcggcaggac aaagtgtgac
     6361 aacaccagag ccagcacatg cagaaacagc agggcgagaa ccgggcccac atcgaacatc
     6421 agaccaccga aggcaatgac cgtgcgaaac ggcttgagaa agacatcgac gggataaaat
     6481 accttcttca cccagggttc ctgtggtcca ggcatgatcc aggtcatcat gacccgcacc
     6541 agaacaaaaa tctgaaagta ttgcaggagc tgcatcagaa gcatgatgtc tttcctcagg
     6601 ccgacggatc gtttccgtgt tcgtcgacga acaaggctcg cccgatgcgc accagagttg
     6661 ccccctcctc aatcgcgatc tcgaaatcgt ccgtcatccc catcgacagt tcgagcgcat
     6721 gatacccggg gccttccagc tcacggcttc tttgaaacaa ctcggccaga ccgcgaaagc
     6781 aacgccgaat catttccaga tcatcagtga aaggtgccat acccatcagg ccctgaacgt
     6841 tcagatcgtc acgtgaccgc agttcacgca agagaggcag aatttcttca ggagcaaacc
     6901 cgtgtttggt gtcttcagga gaaatgtgca cttcgatcag aagcggacag gcccgacctg
     6961 atttcgcggt ccagcgcgcc aggtcttcaa acaaatcacg acggtcgatg gaatgaatca
     7021 tctcagccac cggatagacg tacttgatct tgttgctctg cagcgtgccc acgagatgcc
     7081 agtgaatact cttcaccccg gtgagttccg tcactttgtc gcgcaacgcc tgcggccgat
     7141 tctccccaaa ggttgtctgc ccggcagcaa tcagttgtcg aatctcactc accccgaccg
     7201 tcttggtgat cgccaccagt ttgactgttt caggtgatcg accggctcgt tgcgccgcct
     7261 gtgcaatccg cgttttcaca tcggcgagat tccgcgtaat gtcgatcatg gctgatctcc
     7321 actcagcagc cgagtacgca ccaggttcac actcatgggt tcgactggca gacgatcgca
     7381 gattcccggc acccacttga tatgaatccc ttccggtgcg gcatgctcga tccaccgact
     7441 caaactgcct tcgatgcatg caccgtgaat ggtcagcgac acctgcgctc cagacagcag
     7501 attgcgcggc accagaagct catcgccctg cgcttttctc gaccaggcgc cataccagcc
     7561 cgctcccggc ttgtacaaag ccgcgcgcac gatccattgc cgggccgcat cgcgcagatc
     7621 ctccgaaggc gggttcttga gaaagtattc ctcgaggtca cgggtatgaa acgccgttcc
     7681 atcaccggca accaggaaaa gactggttgg agagtccggg ttgatcgatc cggtaatgta
     7741 gatgtgacga ggcgtgacaa tcatcacatc accctcgact tcgccgcgca tcatgaccga
     7801 gagttccgaa aacacgatgt tcacgccctt gagcgaggcg cgatccaggt tgttcacgcc
     7861 atcgaacttc gtggcggtcg aaagtacgat gaacgattca tccacgacat cctgttcggg
     7921 ggtcacgctg gtcggagaaa agtatgtttc ggaatacgtg ctctgagaac cgacatgata
     7981 cgaaacctgc gtcggttcac cacgacaaaa cccctgacca ctggcatcga tgtaaatacc
     8041 cgacgttttc gcatactcgc gcaacgcctg aaagttcggg cgccacgaag ccatgaacgc
     8101 ggttcgagga gtggtcatct catcgacgaa cactccgttc tccggcaggt caccttcggc
     8161 tttgtccagg acgctctgaa tgcgcttgct gttcgaaagc gcgagagcga ccgagtcatg
     8221 ttcgagtgtc ggtttgcttt cgcctcgatg ataaaagaat gacgccgtct gcaccgggcc
     8281 tttgaaaaca tgactgtcaa ccacgattgg cgtctggcag tacagtggtc cctcacaggg
     8341 cgacgcgcca ccccgcttgt gatgaaccgt tccatagttg ccacgcagca cggtctgtcc
     8401 ggcaatcggc gcgaacggat cagcaccccc gggttgaaac attttcacca gcaacccggg
     8461 atcgtgacac acccccagtg aagtcaaagc gccgcgaaca aacagttcac tgttcttttc
     8521 catgaacgcg ctcagtccgc ctgcaagtct cacccatggc tgaatcggtt gtccgtagta
     8581 gtactcgttc aggctattga acaccgcgaa acgctttacc agcggtgtcg ctgcaacgat
     8641 ccccgtcgcc ttgccgatct gattgcgcac ccgcccttcg ctgaccagcc ggaacagatg
     8701 agcttcgtcg atcagcggtg tctcttcgcg atcgaatccc ggcaggcgac gggtctcgtt
     8761 caggcgaaaa ctcgccccgc ttcccgcgct gagcgctcgc cacaacttcg gcggatcagc
     8821 cagatcatgc agccacgaaa aatacgacgg atgcatcgct ccacccggct gattcagtct
     8881 ttcacgcata cggaccaccc caaaatgcaa cccggcttcg gcggccagac gcgcctgttc
     8941 ctcggcgaga cgcaggttcc ccagatcgcg atcggcctgg tagaccctga tcaacagccc
     9001 acccaacagg aacaggaaaa acagcaccgt gatcaacacg ggcaacgaag tcgtcgaaaa
     9061 ggagaaaggc tttttcattg gtttcgcaga tggatcaacc gctcgaacgg ttgaaaaatt
     9121 tcaggtttgt cgggcaactg tacgcgacac cgtagcttca gcagacgcgg ggccacgcgt
     9181 tcaaaaacgc cgccctgaac tcctgatatc aacggattcg tgagatttcc gatcggtcga
     9241 ggtgccggcg tggtgccgga ggcagtgcga atcatgtggt acaaactgtt ttcggcaaac
     9301 aggaagctgt cgatttcgac tgccgcaccc agcgtccagt cagcagtcgc tcgccgaaag
     9361 gcacactgcg gaccttcccc ctgcgaggga aactctacca ccgcggcatt ctccagttcc
     9421 tgcgagagcc ggtcgagtac cgtttcaacc tgacgcgccc agctcgaccg aaactgcgcg
     9481 gatttcatat cgcgcgagct cagaaacgag aaaaatacca gtgaggcggt tccgaccgcg
     9541 agcagcgtga gaagcaggcg aagctgcgtc gtctgctgtc gtttgtaggc tcgtcgggtg
     9601 tttttcatgg tctggtctgg gtaatcgtgc tttcgagaaa cagcgatttc ttgaataagg
     9661 ggtaacccca tcgaacctga gcccgaatca gggtcattcc gggaagctcc gggtgcggca
     9721 gaaactcgac gcgaccttcg atctcgtgat cgccggtcgt caccaatgta cgaaaccctt
     9781 tttcctgaaa cgcgtcacgg aaaggctgtg aacgtgcctc ggccatcagg ttcttcactg
     9841 cgacggccgc gcgttgatac gtttcactgt cccaggcggg aaacgcctgg atctgccaca
     9901 aggaaataaa cggccagacc agaaaggtca gaataccaaa gatcaacagc cagtcgaccg
     9961 tactgcgccc agcacgcgct tcgggtcgcc ctttgccgtt gatatcgttc atgccgttcc
    10021 tttcgcgggt cgtggtcctc agggttgcag agccgcgtat tcttcgagtt cacgcacgta
    10081 gcgctctgcc atcgcgatct tgtcctcgtc tttcacagtc acggtgtaac gtttccagca
    10141 cgcgatcgcc tcgcgatgtt gtcccagtcg ctcatagata atcgccaggt tgtagagcga
    10201 tttgtcgagg taaggatcct gctttaccga agcctgatac gcctcgatcg cttcatcgta
    10261 gcgtcgctcc atgtcaagca ggcaggccag gttatacagg gcctgtgtat tgcgcggata
    10321 aatattcaga atgtcctgat acgacctgat cgcaccgtcg acgttgttgt cgagatacat
    10381 cgcgtaagcc gcattgagct tcttcgacaa agttgaaggc acaaccggaa tggctcgtac
    10441 ggtgcgggtc atggccggag cagccggtac tccgctgcgg aacaatttgt agtagtggtt
    10501 cactttctta acgtagttct tcgtttcgcg aatcggtgga atgccgccat gctgttccac
    10561 ccgacctggc ccagcattgt agccggcgag cgcgtaatca acatttccct gaaatcgatc
    10621 gagcatcatg cgcagatatc tggcgccacc atgaatgttg tcacgcgggt cgaaagagtt
    10681 ctgacatttg accagtttgg cggtgccggg aatcagctgc atcaggccac acgcgccctt
    10741 cgaagagacg acgttcggat cgaaatcgga ttcgactttg atcaccgcct tgatcagata
    10801 ggggtcgaca ccctgataat tggcggcttc attgatgatt tcgctgtagg tctcgtcgtt
    10861 gggtaagaga attttctgcg gggccatctc ggtcaccatg gtcgatgagt tgccgttcca
    10921 gctggtcggc agatcagggc cggcgcggtc tttgcgggta ggcaccacga tctgatactt
    10981 gctgttggga ggattgtcgg tcagaaacag gcgaccgctc tcgtcacgat aggagtagat
    11041 ggcggcttcc gacaccccgg ccccgaggct gaaaaccgta accaacacat atatgaaccg
    11101 tttcaccgag cttcctccat gtacgaagaa tgcacccgca tcttctcatc ggcggaagcg
    11161 acggttcttt tgagggaaaa ttcgggaacg aaaaaggaaa agccccgaaa ccggggcttt
    11221 gagagatggg gaattcttcg aaaaaaagga gggttcgttt acgcttcgac cggggctgga
    11281 ttgaggtgcg cccagctcag cacgcaggtg acaaaatgcc aggcatattc ttcgtcgagc
    11341 gtttttccgg cctcgagatc ggcggccgct ttcttgcaca tccaacactc gcgctgctgt
    11401 gtcttgtaga gatcgagcag atcaatcggc tttggatcag gggacggggt aggaaccacc
    11461 gtcagattcg gcggcggaga taccggtacc ggattcgggg agacattccc ggagagagcg
    11521 acggcaggcg gcacaggaag ggggttcgcc atgtttacct cggacagcgt tggaaagtga
    11581 agcgattata gggcgcagcc gaaacggcgg caagtttgtg aaaaatatgt gtatcaagga
    11641 ctgatgcggg tgtacgcaac ccaaaaaccg aaccagcaaa ggcggtaccg caacttttca
    11701 ctttttcgaa atttttcttc acaagaaaac tctttattga tcagcttttt ggcagttcag
    11761 aacccggacg gacgaaatta tccctctttt caagacagct ggcggggatc cgagggtacc
    11821 tcttcttcct gaaaccggcg aagtgcctca gagagctgtt gttgcaggtg ttgcctgaga
    11881 ctgccgcgcg ccttggccag caactcgttg cggctggcaa cgatcctggc tttttccttc
    11941 tcgacaccat cattcgggtc gaacagtcgc ttgttgtctt cgagaaactt tcgcaggtcg
    12001 atcataacga ctccttgcac cacagctctc cttccactat cgacgtgcgc gaccggatac
    12061 cgaactgttt cgcgtattga cagaccacac gcaggcaccc tagagtggaa catcaccctg
    12121 gaggatcaca tgtggacttt cgccgctcac tctgacgccc tcggattgaa tcgcagcctg
    12181 gtcgtgactg tgcagcatca gcgcctgata ctcgtgcgca ccactgcagg ggtgtttgcc
    12241 ctcgatgacc gctgcccaca tcaggaacag accttcgccg acggagatgt cgaagaacac
    12301 gcgctctgct gcccgtggca ctcagtcaag gtcgatctgc gcaacgggaa agtcctgaat
    12361 gacatggggt ttcgcggttt gcgagacgta ccggtttttg cggtcaaaga ggaagccggt
    12421 cagattctcg tggaacttcc ggccagctga tcgttcaccg gtcagcgaga tcgcttcatc
    12481 atcgcctcgt gatcgtgcgg gtacgttcgg gcaatcgcga tgagcacaca ccgcacaacc
    12541 ggcacgagca acgcaggctc agcgatcttc ttgtcttacg gctgacagca gctgctcctg
    12601 caggtccagc atcttggggt cgagcgcgcc ggtcacgttc aggcgcgcct cgatgcgcgg
    12661 cggcacaaaa ccgatcagct cctggtaaat ctggccacgc tcttcgcgac tggccacggg
    12721 gtcggcgtat tcgcctatgg ctgcttgaac ggtctcgacg ttgacgcggg gtggaatcat
    12781 ggcgatcttt cggtggcaaa acacaagggg tggcaggcgc tgaggcggtg cgcggtcatt
    12841 c
//
LOCUS       DAORHD010000005        12770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_4019_length_12770_cov_3.338498, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000005 DAORHD010000000
VERSION     DAORHD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_12770_cov_3.338498"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKO06_00260"
     CDS             <1..607
                     /locus_tag="PKO06_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV68096.1"
                     /translation="NRPLHYWSFLPEPVRPWFVRRIRIIGPESTGKTVLARKLAAQYQ
                     TVRVSEYARSWIDLHSNLCRAEDFERYVQGQIAAEDALARQANRLLICDTDPFTTQLW
                     SRLLFGHCPAWIEVAARERHYALTLVTSPDTPWVDDGQRYQPDIAGRRAFFADCLAAL
                     DAAHQRYVVLQGNWEQRFHDACAAVNRVLAEGGCLPDESES"
     gene            591..2000
                     /locus_tag="PKO06_00265"
     CDS             591..2000
                     /locus_tag="PKO06_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein CapK"
                     /protein_id="HNV68097.1"
                     /translation="MNPNPDRPLTESERFPLLHERGRAMLQRLLEHPHAPKYTFPCGD
                     RLDAAGLERVQAFRRHLDQNRSWMQSLAEPPWLSAWIKRCVELVPFYRARTCSGITLA
                     EIPTCSRADLMRAPWSFVPDDQSLDDLIVYFTSGTTGSRLFVYSHPVVSSAYLAGLEW
                     LLAQEGIELARGPEHVAIVQVCAQQSTYTHATVASYLDQAGYAKINLNPHDWRDPDDR
                     WRFLEDCRPQVLTGDPVAFLALAASPAQVQPRALVSSAMLLLPETRAVLTRRFGCPVF
                     DTYSLNEARLVAGAKDGRSLPLLAPDLCVEILDPAGQRCPEGVRGEITLTGGRNPFLP
                     LLRYRTGDYAALGFGQKGIELREFEGRAPVVFLDDAGRAVNSIDVTGALRAFPLAQCV
                     VHQLADRSLIVKVRGLGWTEEELRRVLAGVFGKGTPVTIATLSEAETTDGKVLPYKCD
                     VKEMISGRELPNFRWQNTE"
     gene            2010..2606
                     /locus_tag="PKO06_00270"
     CDS             2010..2606
                     /locus_tag="PKO06_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68098.1"
                     /translation="MEREGPVLELLMRRLAECPPEFLGQPLDDAGKGEIDVAALVWDT
                     VHELSGKTLSVSELAKFQLEASRPRKMLNHLRIISATVWLLADGWFRGRSGVTEKVGT
                     LLLFKLVDFSNHVGFAELLSDSDRREELIRRVLQVFGYRPAGETIAQATERLQGLDSA
                     ERAKVLLAAKQAEDRARKIREEAARKAAEEAAASYGRE"
     gene            complement(2612..3346)
                     /locus_tag="PKO06_00275"
     CDS             complement(2612..3346)
                     /locus_tag="PKO06_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68099.1"
                     /translation="MPRHLTKVLLVAICLAGLNRSMPAGAAKDPFQQLGKLLQAKVAE
                     SLTQQFIRFDQKTGLLEFSPACLQLAGQLVQKNDKTFETFSLTPEGNALHFSIKTKAG
                     TAVSASVIPESLELGLEEMAIRGKLPGGLQITSSEDVQKSIGGFFDSLFGAASQITEL
                     MKNFTVEGDTFRLKRPIKASALGRTLRSQIKSGSDGPAGTTSETRTLNMRMESGWLKL
                     ELGQIGGMNTLMKFAIEQLTSKLQGK"
     gene            complement(3363..3770)
                     /locus_tag="PKO06_00280"
     CDS             complement(3363..3770)
                     /locus_tag="PKO06_00280"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNV68100.1"
                     /translation="MSDEVVFYDGRIPTRVFLFQHNLLWLLLLGWNIGLIASYFHSFR
                     WHVRITSQRLVLTRGFVARKDEEVEFFRVKDTSFEQSFLQRLAGVGTLTIISEDATAP
                     ELTMPIHDPSGFREKIRDGVRVERQRMGTKQIE"
     gene            3961..5307
                     /locus_tag="PKO06_00285"
     CDS             3961..5307
                     /locus_tag="PKO06_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNV68101.1"
                     /translation="MKLSGLSRVRCLVVTLMLVSLLVPVWAETAALPAATSTTTGLVD
                     LERLIPDLDLDLRYATTDNFTNHAVYPVERAFLVEPAARALAAVQADLRKDGYGLRVF
                     DAYRPLAVQRKFWSLLPDPNYVADPKTGSRHNRGASVDVTLIDAHGNECEMPTAFDDF
                     SEKAGAFQPVAGVPARMNRQRLQRAMTRHGFKILKSEWWHFDFGEWKQHPLLDLDLDE
                     LAARATTAPGGAVMKDWRLPDADVQAFLKECETKGIGQLMLVVAEKESDPVAMVQCFE
                     KQNDVWVRSLTSRRASIGRKGVAKPGEKREGDGKSPGGLFKIGRAFGYAASATTKMPY
                     IALVEDDIWVDDPKAPDYNLLTKKAKTKATSFETMRRNDDLYKYGFVVEYNTESIVPG
                     HGSAIFIHIQSGIGKPTAGCVAFGEDRLKRLFSWLDPAKHPHIAIGTRESLFLKHP"
     gene            complement(5359..6684)
                     /locus_tag="PKO06_00290"
     CDS             complement(5359..6684)
                     /locus_tag="PKO06_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV68102.1"
                     /translation="MTKHRYLRGQGTKMDDRERQAFLERWFPLVRRLLAESLPIASYS
                     GQEQQQAECLLRFFHEHGLMAALLPRGGVAALSRRLSAGESVSLETIRREVAQARQRR
                     QPVLAYNAHLDIVYPGETSAWTSSPFSYTERDGRIYGRGTCDMKGAMACMAVALMAIA
                     DHPRHPPTAPLVMGCFVTEEECAEGLSFEEIISDLELVPDAVLLGEPSSLEIARGQRG
                     KLEFRVETHGRLAHTSMPEVGKNAAYDLARVALAIERLDHEEFARFGRDPAHLLDRNT
                     LVLTAARSVPFQRNSVPAEAEGFVTVRLAKHGSFDQIMEKLRKAPGWPTDSRASPLVY
                     TTPSWTGRPSAWICEHPAWETESNHDFTDCLRRTCQLVTGTIPCEKIWPFSTDGVTSA
                     GRHGIPTLGFGPGIESVAHIVDEWVAVDHLETALLVYTTLPKHFSGVSA"
     gene            6729..7658
                     /gene="uvsE"
                     /locus_tag="PKO06_00295"
     CDS             6729..7658
                     /gene="uvsE"
                     /locus_tag="PKO06_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HNV68103.1"
                     /translation="MFIGYPCLNWSMNERPNRTFRLASYSDERFRETVAMNLRATEAI
                     LRWNLAEGFGYFRISSDLIPFASHPVCAVDWAAEFADDLARLGAYIRQHNMRIAMHPD
                     QFTLINAKDPEIVARSVKELAWHACFLDAMGLDATARLQIHVGGVYGDHAASRERFVR
                     EFEKLPPAIAHRLTVENDDRHYSLEDCLWISDRTGIPVLFDVFHHSLHGDGRPVSDCL
                     KQAARTWSKKAGRLLVDYSSQQPEKRPGTHADTLDDSDFSQFLAASKGTDFDVMLEIK
                     DKETSARRALALAQSDPRLRKPCGKTERCKPSR"
     gene            complement(7664..8806)
                     /locus_tag="PKO06_00300"
     CDS             complement(7664..8806)
                     /locus_tag="PKO06_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68104.1"
                     /translation="MNTLWNKFLNLSPWVRMLLAVALVLLLSMSIAGVGLYNFVQALK
                     IENEPVRNPPTDVMTSAGQLKPSSTILRFVSAAQTINTLGDRTSIGKTQAQPASLTPE
                     LISAFQNAIATMAATNLADILEQGYRSTGQQDAYNLLLLRERVRCLASFSIMLKREAP
                     TTDVSPILKAMVHDTLLMERVSPLLISKMVSVACGGIVSNMIHQLVATGSLLPSEAGS
                     LRQLIGTFKQYRLSLAEAMIFEARFVETLVLKLYRRAPLGSWLLHTIFGDPMIDYRKV
                     VAECETMDRIAFHAALEKISNPLTKIMIPNLTKAREVFIQKAAWESIVIQELQDLEGA
                     TADKAIDPYTSQALLMKQIDGKNRYYAAGPNARDDGMTDDDVFFAP"
     gene            complement(8803..>8986)
                     /locus_tag="PKO06_00305"
     CDS             complement(8803..>8986)
                     /locus_tag="PKO06_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68105.1"
                     /translation="FHADSDLELPDDESATIPICEILARAVLPLSFHLLAGWFLLMTV
                     GISLPSQVSHEKEISQ"
     assembly_gap    8987..9086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9087..9419)
                     /locus_tag="PKO06_00310"
     CDS             complement(<9087..9419)
                     /locus_tag="PKO06_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68106.1"
                     /translation="MNEQELKNRFQNLRQTAQASKPLTDAIMAGLPEASETGWQAIRQ
                     QVLANGYRALTVFAVCLLIGSLLSPVAQGFDSLISFDRLPKLHSLGTFAFHADSDLEL
                     PDDESATIP"
     gene            complement(9416..9928)
                     /locus_tag="PKO06_00315"
     CDS             complement(9416..9928)
                     /locus_tag="PKO06_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV68107.1"
                     /translation="MQQAERFRSEVQAWQGLVYSVAFGILLNAQDALDITQETFIKAF
                     EEPAFLDEGFNRKSWLVRVARNLALNTRRSLMRKLRSLMQVAGLFPQATEDSVEEQIV
                     RAEDRQELKAVLEKLGPEERELVTLRYAAELSYEEIAAELNIKLGTVMSRLARVKAKI
                     GMALEEENNS"
     gene            10117..10425
                     /locus_tag="PKO06_00320"
     CDS             10117..10425
                     /locus_tag="PKO06_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68108.1"
                     /translation="MELTKTQKFTLVCPFLLPLTVGAFLALAEQGKKSLWSIPCIFLI
                     FLFTRFLLRAFLMGGKLFIVTRDRAMGIFYYLLAPLLYVFVVFPVLLSIGRILVAELL
                     "
     gene            10603..12171
                     /gene="clcA"
                     /locus_tag="PKO06_00325"
     CDS             10603..12171
                     /gene="clcA"
                     /locus_tag="PKO06_00325"
                     /inference="COORDINATES: protein motif:HMM:NF003640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HNV68109.1"
                     /translation="MRKNRRNQLVWAILVGIGAGTVAVAFQEALHVAESLRGRLVAML
                     TAQGSGIALSAGLFAVLCVLVTAWGTRRFCPEAAGSGIPHLKGVLIGVRTMHGIWLIV
                     VKFIGGLLAIGAGLSLGREGPTVQMGGAVGQLFASGSASKTPQSGSLLIAVGAGAGLS
                     AAFNAPLAGFLFVMEELHFEFAPMPYISALAASITADMISRMAAGQLPAFLVFSHSTP
                     PLSALPGFAILGVLSGLLALLFKKSLIGGLRWTNHLSPKAAWFVPVAAGMLAWLAVLY
                     LPEVAGGGHETSENMLNGSILHRGPGLIFLLLAGKFFLTLISYLAKVPGGIFAPMLVI
                     GGLLGLGVGYVHCLILPGLQGVTPEVFAVIGMASFFAGVVHAPLTGLALIIEMTANFK
                     LLFPMMVATLLAFIMSEWWGGEPVYESLLNEDLRRSGPPPVLFQEPLSMHVQVEEESE
                     MVDRRINQIGFPEGVLIVSVNRRGQKFVPSGLTELKEGDALELMISGEVSTCTQEIRD
                     LAHARLKPAGKATS"
     gene            12349..12585
                     /locus_tag="PKO06_00330"
     CDS             12349..12585
                     /locus_tag="PKO06_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015140840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNV68110.1"
                     /translation="MARRIIVNPDVLVGKPILEGTRISVEFVISLLADGWTQEDIVRN
                     HPHLKPEDIISCLKYAAEMLQAEKVYPLPTTKVA"
     gene            complement(12607..>12770)
                     /locus_tag="PKO06_00335"
     CDS             complement(12607..>12770)
                     /locus_tag="PKO06_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HNV68111.1"
                     /translation="ASLDIFWLRDESLEDSASLPEPHILAAEIAEDLESALDQIRDIL
                     TDLDKRANR"
BASE COUNT         2430 a         3583 c         3872 g         2785 t
ORIGIN      
        1 caaccgacct ctgcattact ggtcgtttct tcccgagccg gtgcgtccgt ggttcgtgcg
       61 tcgcattcgc atcatcggcc cggaatcgac cgggaagacc gtgctggcgc gaaagctggc
      121 cgcgcagtat cagaccgtgc gggtgagcga gtatgcccgc agctggatcg atctgcacag
      181 caatctctgc cgcgccgaag atttcgagcg atacgtgcag ggacagatcg cggccgaaga
      241 tgcgctggca cggcaggcca accgattgct gatctgtgat accgacccgt tcaccacaca
      301 gctgtggtca cgtctgctgt tcgggcactg cccagcctgg atcgaagtgg cagcccgcga
      361 acggcactac gcgctgacac tggtgaccag ccccgatacc ccctgggttg atgacggaca
      421 gcgctatcaa cccgatattg ccgggcgacg cgcatttttc gccgattgtc tcgccgcgct
      481 cgatgccgcg caccagcgct acgtggttct gcagggcaac tgggaacagc gattccatga
      541 cgcgtgcgcg gccgtcaatc gcgtgcttgc cgaaggagga tgtctgcccg atgaatccga
      601 atcctgaccg gccgctgacc gagtccgagc gttttccgct attgcacgag cgcggacgcg
      661 cgatgctgca acgattactc gaacatccgc acgcgccgaa atacacgttt ccctgcggtg
      721 atcgacttga tgccgctggg ctcgaacggg tgcaggcctt tcgccgccat ctcgatcaga
      781 atcgatcctg gatgcagagc ctcgctgagc cgccctggct ctcggcgtgg atcaaacggt
      841 gtgtcgagct ggtgccgttc tatcgcgccc gtacctgttc tgggataacc ctggctgaaa
      901 ttccgacctg ttctcgtgcc gatctcatgc gggctccctg gagtttcgtc ccggacgatc
      961 agtcactcga cgatctgatc gtgtatttta cgagcggaac aaccgggagt cgcctctttg
     1021 tctactcgca tccggtggtc tcttcggcgt atctggccgg tttggaatgg ctgctggcgc
     1081 aggagggaat cgagctggca cggggccctg agcacgtggc gattgtccag gtctgtgccc
     1141 agcagagcac ctatacccat gcgacggttg ccagttatct cgatcaggcg ggctatgcga
     1201 agatcaatct gaacccgcac gactggcgcg acccggacga ccgctggcgg tttctcgaag
     1261 actgccgacc acaggtgctg accggcgatc cggtcgcctt tctcgcgctg gccgcgtcgc
     1321 cggcgcaggt gcaaccgcgg gccctcgttt cgtctgccat gcttctgctg cccgagacgc
     1381 gagcagtgct cacgcgacgt ttcggttgtc cggtcttcga tacctattcc ctcaacgaag
     1441 cacgcctggt tgccggggcg aaagatggcc gttcgctgcc tctgctggct cccgatctgt
     1501 gtgtcgagat tctcgacccc gcggggcagc gttgccccga aggcgtgcga ggagaaatca
     1561 cgctgaccgg tggacgcaat ccgtttctgc cgctgttacg ctatcgtacc ggggattatg
     1621 ccgctttagg ctttggccag aaggggattg agctgcggga attcgaaggc cgtgctcccg
     1681 tggtctttct cgatgacgca gggcgcgcgg tgaactccat cgatgtcaca ggcgcgctgc
     1741 gggcgtttcc gctggcccag tgcgttgtcc atcagttggc agatcgttcc ctgatcgtca
     1801 aagtgcgcgg gctggggtgg acggaagaag aactgcgccg ggttttggcg ggggtgtttg
     1861 ggaagggaac cccagtgacg attgcaacat tatctgaggc ggagacgacc gacggcaagg
     1921 tgctaccgta caagtgtgac gtgaaagaaa tgatctcggg aagagaactg ccgaactttc
     1981 gttggcagaa tacggagtga acactcatca tggaacgtga aggaccggtt ctcgaactgt
     2041 tgatgcgccg cctggccgag tgtccgccgg agtttctcgg ccaacccctc gatgacgccg
     2101 gcaaagggga gatcgatgtg gccgcactgg tctgggacac ggtgcacgag ttgtccggga
     2161 agactctgtc ggtgtcggaa ctggcgaagt ttcaactgga agccagtcga ccccgcaaga
     2221 tgttgaacca tctgcgaatc atcagtgcga ctgtctggct gctcgccgat gggtggtttc
     2281 gtggtcgctc cggggtgacc gagaaagttg gaacgctgct gctgttcaaa ctggtcgatt
     2341 tttcgaatca cgtcggcttt gccgagcttc tgtccgactc ggatcgacgt gaagagctga
     2401 tacgtcgcgt attgcaggtg tttggctacc gccccgcggg ggagacaatc gcgcaggcca
     2461 ccgaacggct gcagggcctc gacagtgccg agcgggccaa agtgctgctg gccgcaaaac
     2521 aggccgaaga ccgagctcgc aagattcgcg aagaggctgc ccgaaaggcg gcagaagagg
     2581 cggcggcatc atacggccgc gaatagtgct tttacttccc ctgaagtttc gaggtgagct
     2641 gctcgatggc gaacttcatc agcgtgttca tgccgccgat ctgtccgagt tcgagtttca
     2701 gccagccgct ttccatgcgc atgttcaggg ttctggtttc gctcgtggtg ccggccgggc
     2761 cgtcactgcc gctcttgatc tgactgcgca gggttcgtcc cagcgccgac gccttgatcg
     2821 gtcgcttcaa tcggaacgtg tcaccctcga cggtgaagtt cttcatcagt tcggtgatct
     2881 gcgacgcggc gccgaacagt gagtcgaaga agccgccgat tgacttttgt acgtcttcac
     2941 tgctggtgat ctgcagaccg cctggcagtt tgccgcgaat ggccatctct tcgagtccga
     3001 gttccagaga ttccggaatc accgaggcac tcaccgctgt gccggctttg gtcttgatgc
     3061 tgaaatgcag ggcattgccc tcgggtgtga gactgaaagt ctcaaaggtt ttgtcgttct
     3121 tctgtaccag ttgtccggca agctgcaggc aggccggaga gaattcgagc agaccggtct
     3181 tctgatcgaa acgaatgaac tgctgggtca gtgactcggc gactttggcc tgcagcagct
     3241 tgcccagctg ctgaaagggg tctttggcgg cacccgcggg cattgatcga ttcagccctg
     3301 ccaggcagat cgcgaccaga agaaccttcg tgagatgacg cggcatgaca aacctccggt
     3361 gattattcga tctgcttggt ccccatgcgt tggcgctcga cccgcacgcc gtcgcgaatc
     3421 ttttcgcgaa agccgctggg atcgtgaatc ggcatggtca gttccggggc ggtcgcatct
     3481 tcggaaatga tggtcagcgt gccgactccg gcgagacgtt gcagaaaact ctgctcaaac
     3541 gaggtgtctt tgacgcggaa gaactcgacc tcttcatcct tgcgggccac gaatccgcgt
     3601 gtgagtacca gtcgctgcga cgtgatacgc acatgccagc gaaaactatg aaagtatgag
     3661 gcaatcaggc caatgttcca gcccagcaac aacagccata gcagattatg ctgaaacaga
     3721 aagacccggg tgggaatccg gccatcgtaa aaaacgactt cgtctgacat acggcatcct
     3781 ccacgaactc acgagatctg ccggcattgt aacacgagaa cgcttccggt ttgcatcctc
     3841 cccgccggct gcggtagtct gtgcccgagt cgaaggcttc ggccacctcg tccaaaccgg
     3901 ggggtacgtc gaaaccgctg acgaggtcga acttccggtc ggtcgaaaag gatgaagcag
     3961 atgaaactct ctggtctgtc tcgcgtgcgt tgtctggtcg tgacgctgat gctggtgtcg
     4021 ttgctggttc ccgtctgggc tgaaaccgct gcactgcccg cggcgacttc caccaccacg
     4081 gggctggttg atctcgaacg gttgattcca gatctcgatc tcgacctgcg ctatgcaacg
     4141 accgataact tcacgaatca tgccgtgtac ccggtcgagc gggcatttct cgtcgaaccc
     4201 gcggctcgcg ctctggccgc tgttcaggca gatctgagaa aagacggcta cggcctgcgt
     4261 gtttttgatg cgtaccggcc gctcgcggta cagcgcaagt tctggagctt gttgccggac
     4321 ccgaactatg tggccgaccc gaaaaccggc tcgcgccaca atcgaggggc ttccgtcgac
     4381 gtgacgctga tcgatgcgca cggcaacgag tgtgagatgc cgacggcgtt cgatgacttt
     4441 tcggaaaagg ccggggcctt tcagccggtg gccggggtgc cggcacggat gaaccgacag
     4501 cggttgcaac gtgcgatgac cagacacggg tttaaaatcc tgaaatccga gtggtggcac
     4561 ttcgacttcg gtgaatggaa gcagcacccg ctccttgatc tcgatcttga tgagctggcc
     4621 gctcgtgcca caactgctcc cggaggtgcc gtgatgaaag attggcgtct tcccgatgct
     4681 gatgtgcagg cttttctgaa agagtgtgag accaagggaa ttggtcagct gatgctggtc
     4741 gtggcggaaa aggagtctga tcccgtggcg atggtccagt gtttcgaaaa gcagaacgac
     4801 gtctgggtgc gaagtctgac cagtcgacga gccagtatcg gccgcaaggg cgtggcaaag
     4861 ccgggcgaga aacgcgaagg tgacggaaag agcccgggcg gtctcttcaa gatcggtcgc
     4921 gcattcgggt atgccgcgtc ggcgacgacc aagatgccgt atatcgcctt agttgaagat
     4981 gatatctggg tcgatgatcc gaaagcgccc gactacaatc tgctgaccaa gaaagcgaag
     5041 acgaaagcga cttcttttga gacgatgcgg cgaaacgatg acttgtacaa gtatgggttc
     5101 gtggttgaat acaataccga atctatcgtt cccggtcatg gcagcgcgat cttcattcac
     5161 attcaatcgg gcatcggcaa gcccaccgcg ggctgcgtcg ccttcggaga agaccgcctg
     5221 aaacgcctgt tctcgtggct cgacccggcg aagcacccgc acatcgccat cggcacccgc
     5281 gagtccctgt ttctcaagca cccctgaaag ttgactcgcg agtgtgacgt cttcgcggct
     5341 gcatgcctga ggcgcgtgtc aggctgacac accggagaag tgtttcggca gggttgtgta
     5401 gaccagcagg gcggtttcga ggtggtcgac ggcgacccac tcgtcgacga tgtgggcgac
     5461 cgattcgatg ccgggtccga acccgagggt tggaatgccg tggcggccag cgctggtgac
     5521 cccgtcggtc gaaaacggcc agatcttctc gcacggaatt gtcccagtta ccaactgaca
     5581 ggtccggcgt agacagtcgg taaagtcatg gttcgattcc gtttcccagg ctgggtgttc
     5641 acagatccac gcgctgggtc ggccggtcca ggatggagtc gtgtagacca acggactggc
     5701 tcgagagtcg gttggccatc ccggagcctt tcgtagcttt tccatgatct gatcaaaaga
     5761 accgtgtttg gcaagtcgta cggtcacgaa cccttcggct tcagctggca ccgagttccg
     5821 ttgaaagggc acagatcgtg ccgccgtcag taccagggtg ttgcggtcga gcaggtgcgc
     5881 ggggtcacgg ccgaagcgtg cgaactcttc gtgatcaagg cgttcgatcg cgagtgcgac
     5941 gcgggcgagg tcgtaggcgg cgttcttgcc gacttcgggc atcgaggtgt gagccagacg
     6001 accgtgtgtc tcaacccgga actcgagctt gccgcgctga ccacgggcga tttcgagtga
     6061 cgacggttcg ccgagcagca ccgcgtcagg tacaagttca agatcactga taatctcttc
     6121 aaaagagaga ccttcggcgc attcctcttc ggtgacgaag catcccatga cgagcggcgc
     6181 ggtcggggga tgccgggggt gatcggcaat ggccatcaag gcaactgcca tgcaggccat
     6241 cgcacctttc atgtcgcagg tgccgcgccc atagattctg ccgtcgcgtt cggtatacga
     6301 aaacggcgac gaggtccagg ccgatgtctc gcccggatac acgatatcga ggtgcgcgtt
     6361 ataggcaaga actggttgtc ggcgttgcct ggcctgggcg acctcacgcc tgatggtctc
     6421 gagagatact gactcacccg ccgacagacg ccgcgagagg gccgcgactc cgccgcgcgg
     6481 caagagggcc gccatcaggc cgtgctcatg aaaaaatcgc agtaggcact cggcctgctg
     6541 ttgctcctgc cccgaatatg aagcgatcgg cagactctcg gcgagcaacc gccgcaccag
     6601 cggaaaccac cgctccagaa acgcctgccg ctcacgatca tccatcttcg tgccttgtcc
     6661 tcgcagatat cgatgcttcg tcatgatact cctattttgg atccccttcg tcgtggtaat
     6721 ctgttgatat gttcatcggg tatccgtgcc tcaactggag catgaatgag cggccgaacc
     6781 gcacctttcg gctggcgtcg tattccgacg agcggtttcg cgaaaccgtg gccatgaacc
     6841 tgcgggccac cgaagctatt ctgcgctgga atctggccga ggggttcgga tactttcgca
     6901 tctcgtctga tctgattcct ttcgcgtcac atccggtctg cgccgtcgac tgggccgcgg
     6961 agttcgctga cgatctggcg cgactcggcg cttacattcg tcaacacaac atgcgcatcg
     7021 cgatgcaccc cgaccagttc acgctgatca acgccaaaga tccggaaatc gtcgcccgca
     7081 gtgtgaaaga gctggcctgg cacgcgtgct ttctcgacgc gatggggctc gatgcgacgg
     7141 cgcgactgca gattcacgtc ggcggcgtct acggcgatca cgccgcgagc cgtgagagat
     7201 tcgtgcgcga gttcgagaag ctgccgcccg cgattgccca ccgcctgacc gtcgagaacg
     7261 acgatcgtca ctactccctc gaagactgtc tctggatcag cgatcgaacc ggcattccgg
     7321 tgctgtttga cgtttttcat cattcgcttc atggcgatgg ccgcccggtg agcgactgtc
     7381 tgaagcaggc ggctcgtacc tggtcgaaga aagcaggtcg actgctggtc gactattcga
     7441 gccagcagcc ggaaaaacgg cctggaacgc acgctgatac tctcgacgac tcggactttt
     7501 ctcagtttct tgcggcaagc aaagggactg acttcgatgt catgcttgag atcaaggaca
     7561 aggaaaccag cgcccgccgc gcccttgccc tcgcgcagtc cgaccctcgc ctgcggaagc
     7621 cctgtggcaa gaccgagcgc tgcaagccat cacgctgaaa tcatcacggc gcgaagaaca
     7681 cgtcgtcgtc tgtcatgccg tcgtcgcgag cgttcgggcc ggcggcatag taccggttct
     7741 tgccatcgat ctgtttcatc aacagtgcct gtgaggtata cggatcaata gccttgtcgg
     7801 ccgtagcgcc ttcgagatcc tgcagttcct ggatcacgat gctctcccag gcggccttct
     7861 gaatgaagac ctcacgggct ttcgtcagat tcgggatcat gatcttcgtg agtggattgg
     7921 aaatcttttc cagcgcggcg tgaaacgcta tccgatccat ggtttcacat tccgcgacga
     7981 ccttacggta atcaatcatc gggtcaccga agatggtgtg cagaagccag ctgccgagag
     8041 gagcacgccg gtaaagcttc agcacgagtg tttccacgaa cctggcttcg aaaatcatgg
     8101 cttccgcgag actgaggcgg tactgtttga acgtaccgat gagctgtcgc aaagagccag
     8161 cttctgaagg gagaagagac cctgtagcca ccagctgatg aatcatgttc gacacgatgc
     8221 cgccgcaggc gacgcttacc attttcgaga tcagaagggg cgagactcgt tccatgagca
     8281 aggtgtcgtg caccatggct ttcagaatgg gcgagacatc tgttgtcggt gcttcgcgtt
     8341 tcagcatgat cgagaatgac gccaggcacc tgacgcgctc tcgcaacaga agcaggttgt
     8401 acgcatcctg ttgcccggtg cttcggtatc cctgttcgag aatgtcggcc aggtttgtgg
     8461 ccgccatggt agcgatggca ttctgaaaag cactgatcag ttcaggggtt aacgaggccg
     8521 gctgagcctg cgttttgccg atggaggttc ggtcgccgag tgtattgatc gtctgcgcgg
     8581 ccgagacgaa tcgcagaatg gtcgaagacg gcttcaattg accggcgctg gtcatcacgt
     8641 cagtcggtgg gttgcgcaca ggctcgtttt caatcttcag agcctggaca aagttgtaca
     8701 atccgacccc cgcgatgctc atcgagagca gaagaaccag ggccacggcc agaagcatgc
     8761 ggacccacgg agacaggttc agaaacttgt tccacaacgt gttcattggc tgatctcctt
     8821 ttcgtgagaa acctgagatg gcaggctgat tccaacggtc atgagcagga accacccggc
     8881 gagaagatga aacgaaagcg ggagaacggc gcgcgccaga atctcgcata tcggaatcgt
     8941 cgcggactcg tcgtctggaa gctccagatc agaatctgcg tgaaagnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnncgga atcgtcgcgg actcgtcgtc tggaagctcc
     9121 agatcagaat ctgcgtgaaa ggcgaaggtg cccagcgaat gaagtttcgg gagacggtcg
     9181 aaagagatca ggctatcgaa cccttgtgcc accggagaca gaaggctgcc gatgagcagg
     9241 cagacggcaa acaccgtgag cgcgcggtat ccatttgcca gcacctgctg acgaatcgcc
     9301 tgccagcctg tttcagaggc ttcgggcaga ccggccatga tggcgtcggt caacggtttc
     9361 gacgcctgtg ccgtctggcg aaggttctga aatctgtttt tcaattcctg ttcgttcatg
     9421 agttgttttc ctcttcaagt gccattccga tctttgcttt tacccgagcc agcctggaca
     9481 tcaccgtccc gagtttgatg ttcagttcgg ccgcgatctc ttcatacgag agttcggcgg
     9541 cgtaccgcag ggtgaccagt tcgcgttctt ccgggccaag tttctcgagc accgctttca
     9601 gctcctgtcg gtcttcagca cgaacgatct gctcctccac gctgtcttcc gtggcttgtg
     9661 gaaacagccc cgcgacctgc atcagtgaac gaagctttct catcagcgag cgacgcgtgt
     9721 tcagcgccag gttgcgggct acgcgaacca gccatgactt gcggttgaac ccttcgtcga
     9781 gaaaggccgg ttcttcgaag gccttgatga acgtttcctg cgtgatatca agagcatcct
     9841 gggcgttgag cagtatgccg aacgcgacgg agtagaccag cccctgccag gcctgcacct
     9901 cgcttcgaaa ccgttccgcc tgctgcacgc cgtttcacct ttctcggatt ttgcgcttcc
     9961 ggaaacaata acaccgttca cccgaacttt attccagcga ttttttcatc ttgcgacaga
    10021 tgccactctg agataggcgc gatcagtcgc agcaactgtt atcgtggtat gattggggcg
    10081 aaggtgtaca aaacctatgg cggaaaggcg gcgctgatgg agctcacaaa gacgcagaag
    10141 tttactcttg tctgcccctt tctgctgcca ctcaccgtcg gggcttttct ggcactcgcg
    10201 gagcagggga aaaagagtct ctggagcatc ccctgcattt tcctgatttt cctgttcacc
    10261 cgatttcttc tgcgcgcatt cctgatgggt ggaaagctgt tcatcgtcac tcgagatcga
    10321 gcgatgggga tcttctacta tcttctcgcg cctctgctgt acgtcttcgt cgtgtttccg
    10381 gttctcctct cgatcgggcg aatcctcgtc gccgaacttc tctagccaac ctcgatctgt
    10441 ggcgtgtgtg cgcttggcct gcgcctttct gccgagagcc tctcgtgctc ctaccacacg
    10501 agatcgccgg cactggctac ttgcacgggc atgtaacttg ccatttccgc gaatcctgat
    10561 atctttctcg ccgaccctct gtcttggaaa ggaatcgatc ggatgcgaaa aaatcgtcgc
    10621 aatcagctgg tttgggcgat cctggtcggt atcggagctg gaacagtcgc ggtggctttt
    10681 caggaggctc tacacgttgc agaatccctt cgtggtcgat tggttgccat gttgaccgct
    10741 caggggagcg gaattgcgct ttcagctggg ttgttcgcgg tcctgtgcgt gctggtgacg
    10801 gcctggggga cgcggcgatt ctgcccagaa gccgctggca gtgggattcc ccatctcaag
    10861 ggggttctga tcggcgtgcg caccatgcat gggatctggc tgatcgtggt gaagttcatc
    10921 ggcggtctgc tggccattgg tgcggggctt tcgctgggtc gtgaaggacc gacggttcag
    10981 atgggcggtg ctgttggaca gctcttcgcc agtggttctg cgagtaagac gccgcagagc
    11041 ggatctcttt tgatagcggt gggtgcgggc gccggactct cggcggcttt caacgcccct
    11101 ctggcgggat tcctgttcgt catggaggaa ttgcatttcg agttcgcccc catgccgtac
    11161 atttcggcct tggccgcctc cattaccgcc gacatgattt cgcgcatggc agcagggcag
    11221 ttgcccgcat tcctggtttt ttcccattca accccgccat tgagtgcgtt gccggggttt
    11281 gcgattctgg gggtcttgtc gggattgctg gcgctgctct tcaagaagag cctcattggc
    11341 ggattacgct ggaccaatca cctgtcaccg aaagccgcct ggttcgttcc cgtggcggca
    11401 ggtatgcttg cctggttggc ggtgctgtat ctgcccgagg tggcgggcgg tgggcatgaa
    11461 acctcagaaa acatgctcaa cggaagtatt ctccaccggg gtccggggtt gatctttctg
    11521 ttgttggcgg gcaagttttt tctgacgctg atcagctatc tggccaaggt ccctgggggt
    11581 attttcgctc ccatgctggt gatcggtggt ctgctgggcc tgggcgtagg atatgtgcat
    11641 tgtctgattc tgcccggcct gcagggagtg actcccgaag tctttgccgt catcggaatg
    11701 gccagcttct tcgcgggagt ggtgcatgcg ccgctgaccg gactggcgct gatcatcgag
    11761 atgactgcca acttcaaact gctctttccc atgatggtgg ctacgctgct ggcgttcatc
    11821 atgtcagagt ggtggggcgg agagccggtc tatgaatccc tgctcaacga agacttgcga
    11881 cgttcgggcc cgccgcccgt gctctttcag gaacccttaa gcatgcatgt tcaggtcgag
    11941 gaagagtccg aaatggttga ccggcgaatc aatcagatcg gctttcccga aggggttctg
    12001 atcgtcagcg tgaatcgccg cggacagaag ttcgtccctt caggcctgac cgaactcaaa
    12061 gagggtgatg ctcttgaact gatgatttca ggggaagtct ccacgtgcac ccaggagatc
    12121 cgcgatttgg cacatgcccg gctgaagccg gccggaaagg cgacttcctg aaatcgggtg
    12181 gcgtgtaggg cgttagcggt tggcgcgttg gtagaggtcg gtgaggatgt cgcggatctg
    12241 gtcgagggcc gactcgaggt cttcggcgat ctcggcggct caagcggtgc ccgcagtatg
    12301 atatgctcaa gtgagcatgg gtgtgtactt tgagactgga ggtgtttcgt ggctcgacgc
    12361 attattgtaa atcccgatgt acttgttggt aagcctatct tggagggcac tcggatttct
    12421 gtcgagttcg tgatcagttt gctggcagac ggttggacac aggaagacat tgtcagaaac
    12481 catccgcacc tgaaaccgga agatatcatc tcgtgtctca agtatgccgc tgaaatgctg
    12541 caagcagaaa aagtgtaccc gttgccgaca accaaggtcg cgtgaaacgt ttcctcgccc
    12601 cacacatcag cggttggcgc gtttatcgag gtcggtgagg atgtcgcgga tctggtcgag
    12661 ggccgattcg aggtcttcgg cgatctcggc ggcgaggatg tgcggctcgg gcagagaggc
    12721 cgagtcttcg aggctctcgt cgcgaagcca gaaaatgtcc aggctggctt
//
LOCUS       DAORHD010000006        11764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_4614_length_11764_cov_4.121530, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000006 DAORHD010000000
VERSION     DAORHD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 11764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11764
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4614_length_11764_cov_4.121530"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..922)
                     /locus_tag="PKO06_00340"
     CDS             complement(29..922)
                     /locus_tag="PKO06_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68112.1"
                     /translation="MSSKHRGLAGRTSASRQGWRVLVGSLCLLGATLLLAGCNVFDTI
                     DTSLVTRPIEDRIAEGKLALASGEFSRAMEIFNALYSEGERSSEVCIGRGEAMAGAAG
                     FNLLTALDILQNGTGPYDHAPVIFGLARMIADRDLLAQGIKALRDAPSVPRADRLARG
                     LWALAWSARLLLDKFDTNHNGRFDHADQIDYTVNDARVSAWPQMYQNLVSGPAPAAQT
                     LEQTYVDLFEGFNGRGTTWTFLTPVADTSLSGTFSESNRNTILAVGDLVERLDLVNLY
                     YETNEASFTTAVRNLDGAEAP"
     gene            1088..2002
                     /locus_tag="PKO06_00345"
     CDS             1088..2002
                     /locus_tag="PKO06_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNV68113.1"
                     /translation="MFSTPIRRRVSALIAALVLLLSPVLFAAPYGDTAVVTGDAADAA
                     GSTSAPTTGVTVPAAGFPVKIATYNIAHARGKETGGLDEVAKYKFLDGIADLLKQEKV
                     EVVGFTEISGGRNLRAKFRDQPQYLAKKLGFHHCYGENVKKGWFGILATQGNVIMSKY
                     PILSWKNHKLYRSDPKHEQRACVEALLDLGKERRLRVFCAHLSTQKEESTKQVDELWG
                     LIKNCKEPVILMGDFNLRPTHEAIIKLSKLMKDTTANLKTSHRGGTNVKIDYHFTLNG
                     ITPGLAYNRGEKEGYSDHLCVINQYWLK"
     gene            2215..3339
                     /locus_tag="PKO06_00350"
     CDS             2215..3339
                     /locus_tag="PKO06_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV68114.1"
                     /translation="MNRLLTGFLVVTAFALALGCGGGGSSPTNAIIAPQIVSINPATG
                     GPGTIVQIQGSGFGSVQGASTLSFSGVTIVPNSWSDNVISATIPTNAAADGSFVISVN
                     GRISNPSTIFTLGNPTIYSLSPTYGNPGTLVTIYGNNFGTLTGESRVTFNGQTATVSS
                     WQPNQITCTVPTSLTVSGDVSVVVYANLSRPSNTISFTLTIPTITSISPQTDNVGAAI
                     IINGSGFGFGPTQQTSTVTISGIPATIVYWTDTYIQVKIPNGVAAGANNVIVTVNGRT
                     STPSSVTVSAPAINTLSPSPVKKDQWITLSGQYFGQTQAEGTSTIFIQGHGLVVTQIN
                     WSDSSISFAWPLNKTDQVPVTITVGGLYTTVLVASEWSLF"
     gene            3368..4249
                     /locus_tag="PKO06_00355"
     CDS             3368..4249
                     /locus_tag="PKO06_00355"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HNV68115.1"
                     /translation="MIISASRRTDVPAFFGEWLVNRLRAGYCKVRNPFNPHQVNRLEL
                     TPETVDAIVFWTRDPRPFTRALEELDARGFRYVFLMTITGYPRELEAATPSLDDQIEA
                     LQQLEQRIGCTRIAWRYDPILLSNHTNESWHLDNFSKIGEKLATAIDHGIISLIDYYR
                     KSERHLKPLEATGWKFRREPDAGPAFDDFVRHLAERAKAIGVRLQTCCESHPSFAAAG
                     ILAGSCIDQEWLNRSLSLHLKQTGRDRGQRPGCLCAPSKDLGSNHTCRHACRYCYATQ
                     SHDPAKLRQHDPLTEFL"
     gene            4285..5073
                     /locus_tag="PKO06_00360"
     CDS             4285..5073
                     /locus_tag="PKO06_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HNV68116.1"
                     /translation="MSLVYHRDMHTLLSRLFVVLYLVFLVPHDVFAWRKWEHVNPADL
                     EVRLVADQPEIVAGETGTFTLILRNRTDKPVSIDFATGQRWDLAVYHGNVQIWQLSNG
                     LQWIESPHSISVRPGEPEIVKMSWTSIDRVGMPLPQDVYRAQALVMCSPRHLVSNKCQ
                     FRLLPPKNTGQGVTRVPVGKHFEIVLPRFLDGVEVDWRPDYDYNDNRVSEIEYRKTAK
                     ETIWTLRADRPGHVNLHMYGFVPFREQMRSKERRSYRIEVFLNE"
     gene            5066..8602
                     /locus_tag="PKO06_00365"
     CDS             5066..8602
                     /locus_tag="PKO06_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV68117.1"
                     /translation="MSKTTSERPASGRAHTVNPAGKKNVEALMLEKGVISAEQLEKAK
                     NIQAQTLERLEDIIVRLGFLSEKEILDLWAAFLDTSVVDLTNIKVDAKILSLIPEYQA
                     KQYKVIPYKREGNKLTIVMADPFDIMASDMIAFLTQCKLEVLIAPKSQIDLAIKQLYS
                     WEGFSVTGEETLLTRMDDQTPRDPGTELARRTSEEGPVVQFLNAIFKQAILKRASDIH
                     IEPREDILLVRFRIDGLLHDAMTGPKSLMAPLVSRVKILSHLDIAERRMPQDGRLKVK
                     MEGKEVDFRVSTVPSLLGEKAVLRLLQRESAYDLDNIGFLPDDLERLKSALDEPYGMV
                     LLTGPTGAGKTTTLFGMLKYFNSSQINIFTIENPVEYRMDGITQVQINEKIGLDFPQG
                     LRAALRQDPDVILVGEIRDLETAEIAFRASLTGHKVLSTLHTNNAAATINRLINMGVP
                     AYLVTAALRAVIAQKLLHRICPKCRTEYRPSQKTFQKLKLPPSKTMSTKFFRGTGCRD
                     CNMTGAYGRLGVFETMVITSAIRDLIMEGGTEMLIKRGSRLGGMKTMREIALQKCFDG
                     LTTLEEVLYSTPSDDEPIYSGIAAGPGSGRSIIMPIAMPVAAPVAGIAHQDISTQAHT
                     EAFDANDPLMRQALDKLEEEFLFFTDEELMARLQVPLGQLQEMALSVGVERTADFFRK
                     RFGKLPEEMSEDDLINLIRNVQEKQGRMPEFLFESDASAQALAKAMTSEAVRLLSERV
                     SEPQKLPQYFTEDFCRECGLDLALDWAHGSRLKLIDLVLPGQYRPWQFGETGAWLATG
                     DIGQKVREALDWLMTTRLGCSRGDLPNMLTSDVVKEVGLGALLEKFGGSVYRLVQAAY
                     PGIFMPWQFPEEESLIWFREDRNEIAQAAVRWLVDTQLKITPDQIPARIGLKDFHGAG
                     LSKLLDLFNQNLFQVVDNAFPERFKPWEFAEQGDLWRNTNALETARKAVSWLIQERLQ
                     WEPTAAISQLTRRHFLQHGLGFMLGTLFNHSPLYALENAFEALKTNEIFQKTLIAFSQ
                     EVRDLSSKWASLAEKIALNQFGKARFKVALPNLKVAPILPENERIYYNASNQIEYVPQ
                     IIVTKLSAYDDYADFANWVPYCDRLIYWVLFDDRTEPYLGPAHPKVKLMFSENLVSGL
                     RNRGMLDVVEKIHTLRGSFERLLDRKAPAIGG"
     gene            8610..9512
                     /locus_tag="PKO06_00370"
     CDS             8610..9512
                     /locus_tag="PKO06_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68118.1"
                     /translation="MLVFQRSEAGTIREQNQDLLLAREEHGIFILADGEGANGRAAAE
                     QAAERLASDLLRLKALSSPAYAADAFPRLIEQTARSLQQSRIQQPEMANTSVSLGICF
                     IHEARLFVATTGRVGALIHLGSDVHTLEPHGIPPLAATLADDRLNGTTSHHPTRACNP
                     DPTYSTSSSVSEESFTISQLGPLSFQVGDWVLFCSQGLLISQPLAEIVPIAPAVHEDA
                     ENLANALFQRASARYDGDDRSLALLRFLPADLQLRFPPERVLSVDLDKRWKAPLWIPL
                     ALLGICTCLGVWLKKRLSVFLSED"
     gene            9560..10762
                     /locus_tag="PKO06_00375"
     CDS             9560..10762
                     /locus_tag="PKO06_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNV68119.1"
                     /translation="MSTESIFRSGLEGKSDVIVTYEKTDATLEIVVESSVKKLFGRHI
                     RERAEKSCREFGVTKGKLHIKDDGALDYVLMARLEAVFRKAGVYTPAPFKALPRAAGA
                     KERLRRSRLYLPGNQPDLSVNAGLFGGDSNILDLEDSVAPDQKLDARVLVRRTLEMAD
                     RFFPQGEIIVRMNPLSGPFGRDDLREIVPAAPHTLLIPKCESVEDIKAVESAVAEIEK
                     ASGMTVGRTLFMPLLETARGVMNAHAVATASSRNVALCFGAEDFTRDLGIPRTVTGQE
                     TLQARATVVMGSRAAGLDAIDTVFSDVADEKGLYNSALEAKAMGFVGKGVIHPRQIPV
                     VHQAFQPSDAELEEAKKIVLALREAEKTGSGVVALGSKMVDAPVAARARKLIDLARKM
                     NLPGLEEF"
     gene            10765..>11764
                     /locus_tag="PKO06_00380"
     CDS             10765..>11764
                     /locus_tag="PKO06_00380"
                     /inference="COORDINATES: protein motif:HMM:NF016138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HNV68120.1"
                     /translation="MNWTTNSIGRRIPTEIEGRKLTPYQGAFSHRPSGRIAGCPIPTT
                     THRGRIDKVVTNWDALLDKLPLRDGITISFHHHLRNGDQVVNEVVKRLAARGLKNLVI
                     APSALFPTHDELVPFIESGVISHVEGSMNGQVGVACSRGKMKHTAILRSHGGRYRAIQ
                     DGDLHIDIAFIAAPTADEFGNATGVHGKSACGPMLYALPDSLWADTVVVITDNLVRYP
                     CIPWSIQGGNVDHVLTVESIGDPTKIVSGTTKLTKSPTQLLIAEMTAQFIEEAGIMKD
                     GFSYQAGAGGISLAATVFLAERMRKKSVKMSFAHGGATQMLVDLLNEGLIGHILDLQ"
BASE COUNT         2615 a         3517 c         3196 g         2436 t
ORIGIN      
        1 gcctgaatct gcgttgtcgt gaagaatctc atggggcttc cgctccatcg agattgcgta
       61 cagccgtggt aaagctggct tcgttggttt cgtagtacaa gttcaccaga tcgagacgct
      121 cgaccagatc tccaacagcg agaatggtat tgcgattcga ctcggagaag gttccgctca
      181 acgaggtgtc agccaccggg gtgaggaaag tccaggtcgt tccccgcccg ttgaatcctt
      241 cgaaaagatc aacgtaggtc tgttccagcg tctgggccgc aggagccgga ccggaaacca
      301 ggttctgata catctgtggc cacgccgaga cgcgggcgtc attcacggtg taatcgattt
      361 gatcggcgtg atcgaatcgt ccgttgtgat tcgtgtcaaa cttgtccagc aacaggcgag
      421 cgctccaggc cagcgcccag agaccccggg ccaaacggtc ggctcgcggc acactcggtg
      481 cgtcacgcaa cgccttgatt ccctgcgcta acaggtcgcg gtccgcgatc atgcgtgcca
      541 ggccgaagat gaccggagcg tgatcatacg gcccggtgcc attctgcaga atgtcgagag
      601 cggtcaacag attaaagccc gccgcaccgg ccatcgcttc acctcgaccg atgcacacct
      661 ctgaagatcg ctccccttcg gaatacaggg cgttgaagat ctccatcgcc cgcgaaaact
      721 cccctgaggc cagagccagc tttccttccg caatgcggtc ttcgatcggg cgggtaacca
      781 gtgacgtatc gatcgtgtcg aacacgttgc atccggccag caacagggtc gccccaagca
      841 ggcagagcga cccgacaagc acgcgccacc cctgccgcga ggctgaagtg cgacctgcca
      901 aaccacggtg tttcgagctc atgcttcgtt ccgatacgag agaaggtaaa tgaaatcgag
      961 gacacaacga cacgattcca cgtcattcat cggcagatca tcgatcaatg tgaatcgttc
     1021 cgatacagaa aacctttcag gttttgcgtg gtaacatgga gtgaatatgg ttcaagggag
     1081 gtcttgcatg ttttcgacac ccatccgccg tcgagtgtcc gccttgatcg cggcactggt
     1141 tctgctgttg tccccggttt tgttcgccgc cccgtacgga gataccgcgg tcgtcaccgg
     1201 cgacgcagcc gatgccgccg gctcgacgag cgcgccaaca acgggagtga ccgttcctgc
     1261 ggcgggtttt cccgtcaaga tcgcgaccta taacatcgcg catgcgcgcg gcaaagagac
     1321 aggcgggctc gatgaggtcg cgaagtacaa gttcctcgac ggcatcgccg atctgctgaa
     1381 acaagagaaa gtcgaagtcg tcggcttcac cgagatttcg ggtgggcgga atctgcgcgc
     1441 caagtttcgc gatcaacccc agtatctcgc gaagaaactc ggatttcatc attgctacgg
     1501 ggaaaacgtc aagaaggggt ggttcggcat tctcgccacc cagggcaatg tgatcatgtc
     1561 gaagtatccg attctctcct ggaagaacca caagctgtac cgcagcgatc cgaagcatga
     1621 gcaacgggcc tgcgtcgaag ccttgctcga tcttggcaaa gaacgccggc tgcgggtttt
     1681 ctgcgctcat ctgagcaccc agaaagaaga gagcaccaaa caggtcgatg aactgtgggg
     1741 tctgatcaag aattgcaaag aaccggtcat tctcatgggg gatttcaacc tgcgacccac
     1801 tcacgaagcc atcatcaagc tgagcaaact gatgaaagac acgacggcca atctgaaaac
     1861 cagccaccgt ggcggaacga acgtaaagat cgactaccac ttcaccctga acggcataac
     1921 cccgggactg gcctataacc gaggcgagaa agagggatac tcagatcatc tgtgcgtcat
     1981 caatcagtac tggttgaagt aaccgtcaga gcccactgat cgtgcattta cgccccgcct
     2041 ttaaggcggg gcgtctgctt tttcacggaa acacgatcga tgttttttcg acacaaggtt
     2101 aacgaaacgg cgcaaaaacc cgacgtatga agtgagcctg cggcaacatg tcgtttgttt
     2161 ccgcatgcag acttcattga cgctcatctt gtacgttctt ataatggaaa catcatgaac
     2221 cgactattga ccggctttct cgtggtcacg gccttcgccc tcgctctcgg atgcggtggc
     2281 ggcgggtcga gtccgactaa cgccatcatt gccccacaga tcgtctcgat caaccccgca
     2341 accggcggtc ccggaaccat tgtccagatt cagggcagcg gcttcggcag cgtccaggga
     2401 gccagtaccc tgagcttctc cggcgtgacg atcgttccca acagctggag cgacaacgtg
     2461 atctccgcca ccattccaac aaacgctgct gctgacggtt ctttcgtcat cagcgtcaac
     2521 ggccgcattt caaaccccag caccatcttc accctgggga acccgacgat ctattcattg
     2581 agcccaacct acgggaaccc gggaacactg gtcacaattt acgggaacaa cttcgggacc
     2641 ctgaccggcg aatctcgcgt tacctttaac gggcagaccg ccaccgtcag ttcctggcaa
     2701 ccgaatcaga tcacctgtac agttccaacc tctctcaccg tatccggaga cgtgtcggtc
     2761 gtcgtctacg ccaatctcag ccgaccctca aatacgatct cgttcacttt aaccattcca
     2821 acgatcacct cgatcagtcc tcaaacagac aatgtcggag cagccattat tataaacggt
     2881 tctggatttg gcttcggtcc aacccaacag acaagtaccg ttacgattag cgggattcca
     2941 gccacaattg tgtactggac ggatacttat atccaagtga agattccgaa cggtgtcgct
     3001 gctggcgcta acaatgtcat cgtcacggta aatggtcgta catcaacacc atcgtcggtt
     3061 accgtttcag ccccggcaat aaacactcta tcgccgtcac ccgtgaaaaa ggatcaatgg
     3121 attaccctaa gcggacagta ttttggacaa acacaagccg aaggaactag tacaattttc
     3181 atacagggtc atgggttggt cgtcacgcaa ataaactgga gtgacagcag tatctctttt
     3241 gcctggcccc tgaataaaac agatcaagtt ccggtgacca taactgtcgg aggcctttac
     3301 acaacagtac ttgttgcttc tgagtggagc ctcttctgac acagcgggtc taagacgacc
     3361 tacaaaaatg atcatcagcg cgtcgcggcg gacggatgtg ccggcgtttt tcggtgaatg
     3421 gcttgtgaac cgcctgcggg ccgggtactg caaggtcaga aatccgttca acccgcatca
     3481 ggtaaaccgt ctcgaactga cgcccgaaac ggtcgatgcc atcgtgttct ggacgcgtga
     3541 tccgcgccct ttcaccagag ccctcgaaga actcgacgcc cgcggttttc gctacgtctt
     3601 tctgatgaca atcaccggct atccacgcga acttgaagct gccactccgt ctctggacga
     3661 tcagatagag gccctgcagc aacttgaaca gcgtatcggc tgcacgcgca ttgcctggcg
     3721 atacgatcca attctactct ccaatcatac gaatgaatcg tggcacctcg acaacttcag
     3781 caagatcggt gagaagctcg cgaccgccat cgaccatggc atcatcagcc tcatcgatta
     3841 ctaccgaaaa tcggaacgac acctgaaacc tcttgaagcc acaggctgga aattccgtcg
     3901 tgaacccgat gctggcccag cgtttgacga cttcgttcgt catctggctg aacgtgcgaa
     3961 ggcaatcggc gttcgcctgc aaacctgctg cgaatcgcac ccgagtttcg ccgcagcggg
     4021 aatcttagcc ggctcgtgca tcgatcagga atggttgaat cgatctctga gtctgcacct
     4081 gaaacagacg ggtcgagacc gtggtcagcg ccccggttgc ctctgtgctc catcaaagga
     4141 tctgggcagc aatcatacct gtcgccacgc ctgccgctac tgttatgcga cccagtcgca
     4201 cgatccggcc aagctgcgtc agcatgaccc actcactgag tttctatagg gaattctctc
     4261 tcagaagagt tgttggcagg gagaatgtct ctggtgtatc atcgcgacat gcacaccctg
     4321 ctgtcccggc tgtttgtcgt tctgtacctg gttttcctcg ttccccacga tgtcttcgcc
     4381 tggcgaaagt gggaacacgt caaccccgcc gatctggaag tgaggctcgt tgccgatcaa
     4441 ccggagatcg tcgccggcga aaccggcaca tttaccctga ttctgcgcaa ccgcaccgac
     4501 aaaccggtgt cgatcgattt tgcgaccggt caacgctggg atctcgcggt ttatcacggc
     4561 aacgtgcaga tctggcaact ctcgaacggt ctgcagtgga tcgaatcgcc gcattcgatc
     4621 tcggttcgcc ccggtgaacc cgagatcgtg aaaatgtcct ggacctcgat cgaccgagtc
     4681 ggtatgccac tgccgcaaga tgtttaccgc gctcaagcac tcgtcatgtg cagccctcga
     4741 catttggtct cgaacaagtg tcaattccga ctccttcctc ccaagaatac ggggcagggt
     4801 gtcacgagag taccagttgg aaaacacttc gagatcgttc tgccgagatt tctcgatggt
     4861 gttgaagtcg actggaggcc agactacgac tacaacgaca accgcgtttc cgagatcgaa
     4921 tatcgcaaaa cggccaagga aacgatctgg accctgcgcg ccgatcgccc gggtcacgtg
     4981 aacctgcaca tgtacggctt cgtacctttc cgagaacaga tgcgttcaaa agagcgccga
     5041 tcctatcgca tcgaggtctt tctcaatgag taaaaccact tccgagcgtc cggcttcggg
     5101 ccgggcccac accgtcaacc ctgccggcaa gaagaacgtc gaagctctga tgctcgaaaa
     5161 gggtgtcatt tcggccgagc agctcgagaa agccaagaac attcaggcgc aaacgctcga
     5221 acgcctcgag gacatcatcg tgcgtctggg ctttctcagc gagaaagaga ttctcgatct
     5281 ctgggccgcc tttctcgaca cctcggtggt cgatctgacc aacatcaagg tcgatgccaa
     5341 gatcctgtcg ctgattcctg aatatcaagc caaacagtat aaggtcattc cctacaaacg
     5401 cgaaggcaac aagctgacga tcgtcatggc cgacccgttc gacatcatgg ccagcgacat
     5461 gatcgctttt ctcacgcagt gcaagctcga ggtgctgatc gcgcccaaaa gccagatcga
     5521 cctcgctatc aagcagctct attcctggga aggattttcg gtcaccgggg aagagaccct
     5581 gctgacgcgc atggatgatc agacgccccg cgacccgggc accgaactcg cccgccgcac
     5641 gtctgaagag gggccggtcg ttcagtttct gaacgcgatt ttcaagcagg ccattctgaa
     5701 gcgcgcgtcg gacattcaca tcgagccgcg cgaggacatt ctgctcgttc ggtttcgcat
     5761 cgacggtctg ctgcacgacg ccatgaccgg gccgaaaagt ctgatggcgc cgctcgtttc
     5821 gcgcgtcaag attctgtctc acctcgacat cgccgagcgc cgcatgcctc aggatgggcg
     5881 tctcaaggtg aagatggaag gcaaggaagt ggacttccgt gtcagcacag tgcccagcct
     5941 gctgggcgag aaagccgttc tgcgcctgct gcagcgcgag tcagcctacg atctcgacaa
     6001 catcggcttt ctgcccgacg atctcgagcg gctgaaaagt gctctcgacg agccgtacgg
     6061 catggtgctg ctgaccggtc cgaccggcgc gggcaagacc acgacgttgt tcggcatgct
     6121 caagtatttc aactcgagcc agatcaacat cttcacgatc gaaaacccgg ttgaataccg
     6181 catggacggc atcacgcagg tacagatcaa cgagaagatc gggctcgatt ttccgcaggg
     6241 tctgcgcgcc gcgctgcgcc aggaccccga cgtcattctc gtcggcgaaa ttcgtgatct
     6301 cgaaacggcg gaaatcgctt ttcgcgcttc gttaaccggt cacaaagtgc tctcgacgct
     6361 gcacacgaac aacgccgcgg caacgatcaa ccgcctcatc aacatgggcg taccggctta
     6421 tctggtcaca gccgccctgc gggccgtcat cgcgcagaag ctgctgcacc gcatctgccc
     6481 caagtgccgc accgagtatc gtccgtcaca gaagacgttc cagaaactca aactgccccc
     6541 cagcaaaacg atgtcgacca agtttttccg cggcaccggt tgtcgcgact gcaacatgac
     6601 cggcgcttat gggcgcctcg gcgtcttcga aaccatggtc atcacctcgg ccattcgcga
     6661 cctgatcatg gagggcggca ctgagatgtt gatcaaacgc gggtctcggc tgggcggcat
     6721 gaaaaccatg cgcgagatcg ctttgcagaa gtgtttcgat gggttgacca cgcttgagga
     6781 agttctgtat tcgaccccca gcgatgatga gccgatctac agcggaatcg ccgccggacc
     6841 tgggagtggt cgatcgatca tcatgccgat tgcaatgccg gttgcagcac ccgttgcagg
     6901 aatcgcccac caggacattt caacacaggc gcacacggaa gcctttgatg ccaatgatcc
     6961 gctgatgcgt caggccctcg acaaactcga agaagagttt ctgtttttca ccgatgaaga
     7021 attgatggcg cggctgcagg tgccgcttgg tcaactgcag gaaatggcac tctcggtcgg
     7081 tgtcgaacgc acggctgact tcttccgcaa gcgtttcggc aagctgccgg aagagatgtc
     7141 ggaagatgac ctgatcaatc tgattcgcaa tgttcaggaa aagcagggcc gtatgccgga
     7201 gtttctcttc gagagcgacg cctcggcaca ggcactcgcg aaagccatga cgtcagaggc
     7261 cgtccgtttg ctgagcgaac gtgtcagcga accgcagaaa ctgccgcagt atttcaccga
     7321 agatttctgt cgcgagtgcg ggctcgatct cgctctcgac tgggctcacg gcagtcgtct
     7381 gaaactcatc gatctggttc tgcccggtca gtatcgaccc tggcagttcg gcgagacggg
     7441 agcctggctc gccaccggag acatcggaca aaaggtacgt gaagcgctcg attggctgat
     7501 gaccacacgt ctgggatgca gtcgcggtga tctccccaac atgctcactt ctgacgtcgt
     7561 caaagaggtg ggcctggggg cgctgctcga gaaattcggt ggttcggtct atcggctagt
     7621 acaagccgct tatcctggca tcttcatgcc atggcagttt ccggaagaag aatcattgat
     7681 ctggttccgt gaagaccgca acgagatcgc ccaggccgcc gtccgctggc tggtcgacac
     7741 ccagctgaag atcactcccg atcagattcc ggcccgcatc ggcctgaagg atttccatgg
     7801 ggcagggttg tcgaagctgc ttgatctgtt caaccagaac ctgtttcagg ttgtcgacaa
     7861 cgcctttcca gagcgcttca agccttggga gttcgccgaa cagggagatc tctggcgcaa
     7921 cacgaacgcc ctcgagaccg cgcgaaaagc cgtctcgtgg ctgatacagg aacgtctgca
     7981 gtgggaaccg accgcggcga tctcgcagct gacgcggcgc cactttctgc aacacgggct
     8041 cgggttcatg ctgggcaccc tgttcaatca ttccccgctg tatgcactgg aaaatgcttt
     8101 tgaagcactg aagaccaacg agatctttca gaaaacgctg atcgcctttt cacaggaagt
     8161 gcgcgatctg tcttcgaagt gggcttcact tgccgaaaag atcgcgttga atcaattcgg
     8221 caaagcccga ttcaaggtcg ctctgcccaa tctcaaagtc gcgccgattc tgcccgaaaa
     8281 cgaacgtatc tactacaacg cttcgaacca gatcgagtat gtgccacaga tcatcgtcac
     8341 caagctaagc gcgtatgacg attacgccga ctttgccaac tgggtgccgt attgcgatcg
     8401 gctgatctat tgggttctct tcgacgatcg caccgagcca tatcttggac ccgcacaccc
     8461 gaaggtcaag ctgatgttct cggagaatct cgtgtcaggc ttgcgcaacc gcggcatgct
     8521 cgatgtcgtc gagaagatcc acacgctgcg aggcagcttc gagcgcctgc tcgaccgcaa
     8581 ggctccggcc atcggcggct agacgcatca tgctcgtctt tcaacgttca gaagctggta
     8641 ctatccgcga acagaatcaa gacctactgc tcgcccgcga agagcacggc atcttcattc
     8701 tcgccgacgg tgaaggagca aacggacgcg ctgcggcaga acaggccgcc gaacgtctgg
     8761 ccagcgatct gttgcgcctc aaggccctgt cctcaccagc atatgcggct gacgcgtttc
     8821 cccgcctgat cgagcagacc gccaggagcc ttcaacagtc caggatacaa caaccggaga
     8881 tggctaatac atcagtgtct ctgggcatct gtttcattca cgaagcacgc ctgttcgttg
     8941 ccacaaccgg cagagtcggg gctctcattc atctggggtc tgacgttcac accctcgagc
     9001 cgcacggtat tcctcccctg gctgcgaccc tggccgacga tcgcctgaac ggaacgactt
     9061 cgcatcaccc cacccgcgca tgcaatccgg atccaacgta ctcgacttcg tcgtccgtgt
     9121 ccgaagagtc tttcacgatt tcccaactcg gaccattgtc ctttcaagtc ggtgattggg
     9181 tgctcttctg ttcgcagggt ctcttgatct cacagcccct ggccgagatc gtcccgattg
     9241 ctcctgccgt acatgaagac gcggagaatc tggccaatgc gctgtttcaa cgggcctccg
     9301 ctcgttatga tggcgatgat cgatcgttag cgttgcttcg ctttctcccc gcagacctgc
     9361 agttgcgatt tcctcccgaa cgagttctca gcgtcgacct tgataaacgc tggaaagcac
     9421 cgctctggat tcccctcgct ctgctgggta tctgcacctg tctgggcgtg tggctcaaga
     9481 aacgtttgtc cgtttttctg tccgaagatt gaatcagaaa accgtcagat atcacggaaa
     9541 ggtcggaggt tgatggagaa tgagtactga aagcattttc cgctctggtc tggaaggcaa
     9601 gtcggatgtc atcgtcacct acgagaagac cgacgccacc ctcgagatcg tggtggaatc
     9661 gagtgtcaag aaactgttcg gccgtcacat tcgtgagcga gccgaaaaat cctgccggga
     9721 attcggcgtg accaagggca agcttcacat caaggatgac ggtgctctcg actacgtgtt
     9781 gatggcccgc cttgaggcgg ttttccgcaa agcgggcgtc tatactcccg ccccgttcaa
     9841 agctttacca cgcgcggccg gggccaaaga gcgactgcgc cgcagtcgtc tgtatctgcc
     9901 cgggaatcag cccgatctct ccgtgaacgc cgggttattc ggaggcgaca gcaatatcct
     9961 tgatctggaa gactcggtgg ctcccgatca gaaactcgat gcccgcgttc tggtacgccg
    10021 aacgctggaa atggctgaca ggttctttcc gcagggagag atcatcgttc gcatgaatcc
    10081 cttgagcggc ccgtttggtc gtgacgatct gcgtgagatc gtgccagcgg caccgcacac
    10141 gctgctgatt ccaaagtgcg agtcagtcga agacatcaag gccgtcgaat cagcggttgc
    10201 cgagatcgaa aaggcatcag gcatgaccgt tggacgcacg ctgttcatgc cgctgctcga
    10261 aacggctcgg ggcgtgatga acgcccatgc ggtcgccacc gcgtcatcgc gcaacgtcgc
    10321 gctctgcttc ggagccgaag atttcacgcg cgatctcggg ataccgcgca cggtgacggg
    10381 tcaggaaacc ctgcaggctc gcgccacggt cgtgatgggc tcccgagccg ccggtctcga
    10441 tgccatcgac acggtgttca gcgatgtcgc cgacgagaag ggcttgtaca attcagccct
    10501 cgaggccaaa gccatggggt tcgtcggcaa gggtgtcatc caccccagac agatcccggt
    10561 cgtacatcag gcgtttcagc cctctgacgc cgaactggaa gaagccaaga agatcgtgct
    10621 ggctctgcgc gaagccgaaa agaccggcag cggtgtcgtc gccctcggct caaaaatggt
    10681 cgacgccccg gttgccgccc gggcgcgcaa actgatcgat ctggcccgca agatgaacct
    10741 tcccggactg gaggaattct gaccatgaac tggaccacca actcgatcgg acgcaggatt
    10801 cccaccgaga tcgaaggacg caagctgact ccgtaccagg gcgcattctc gcatcgcccg
    10861 tctggccgca ttgccggatg cccgattccg accaccactc atcgaggtcg tatcgacaag
    10921 gttgtcacga actgggacgc attgctggac aaactgccac tgcgtgacgg catcaccatc
    10981 tcattccatc atcaccttcg caatggagac caggtggtga acgaggtggt caagcgcctg
    11041 gccgcacgcg gtctgaaaaa tctcgtcatt gccccatcag cactgtttcc gacccatgac
    11101 gaactcgtac cattcatcga gtcgggtgtc atttcgcatg tcgaaggttc gatgaacggg
    11161 caagtcggtg ttgcctgctc acgcggcaaa atgaagcata cagccattct gcgctcgcat
    11221 ggcgggcgct accgggccat tcaggatggc gatctgcaca tcgatatcgc cttcattgcc
    11281 gcaccaacag ccgacgagtt cggcaacgcc acgggcgttc acgggaagtc ggcctgcgga
    11341 ccgatgttgt atgccctgcc tgactcgctg tgggctgata ccgtcgttgt catcacggac
    11401 aacctggtgc gttacccctg cattccctgg tcgattcagg gtggcaacgt cgaccacgtg
    11461 ctgaccgtcg aatcgatcgg cgacccaacc aagatcgttt caggcacgac caagctgacc
    11521 aagagcccaa cccagctgtt gatcgcagaa atgaccgctc agttcatcga agaagcggga
    11581 atcatgaaag acggtttttc ctaccaggcc ggtgccggcg gcatctctct ggccgcgacc
    11641 gtgtttctcg ccgaacgcat gcggaagaag agcgtgaaga tgtcgttcgc tcatggcggc
    11701 gcaacccaaa tgctcgtcga cctgctcaac gaggggttga tcgggcatat tctcgatctg
    11761 caga
//
LOCUS       DAORHD010000007        11756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_4622_length_11756_cov_3.913512, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000007 DAORHD010000000
VERSION     DAORHD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4622_length_11756_cov_3.913512"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PKO06_00385"
     CDS             complement(<1..196)
                     /locus_tag="PKO06_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68121.1"
                     /translation="MSDVNSSLDLSPPADRPAAGEASNDAAVELPSTRPFEGWFFLRK
                     EWIDQEEGIEKVTLNYTIGPA"
     gene            complement(327..1604)
                     /locus_tag="PKO06_00390"
     CDS             complement(327..1604)
                     /locus_tag="PKO06_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68122.1"
                     /translation="MAALFLACTAMAQMSEDINEFLTAGKACYQKRDLLGAQTEFENV
                     LLLDPRNLDAMVCLVRVYADRKELRKAQDMLSRARQVNSDHAAVKELTTLLGSDRGHV
                     KAKEADLVTYEALTLLGSGTRQRPYGLVVPETRVKVAAKDPLADEAEKPLSLDPATTS
                     PELQSFTKDEGPLTEVFDLWVSHGLPVALEKYFELILADRSIGAYDDRKLLEEGLTYF
                     NPRLESNKEDPEARFFLGMIAYLNGMRDRTLELFEPLRANPGSHEPLVRKVLKELDDW
                     KAEEERRLAEIKRQKEAEEAAKQAQLLAQQMAASNASAAAAVGSGTAGTSTSGSEMLD
                     NDGYDLYKRGQIEAAIEKFTAAIKENANEPKFHYHLGLALTDKGLNGQVEAFDRAIEA
                     FNQVLRLTPGSKLAQDSETMIRDIVAAKNTLKK"
     gene            complement(1663..2958)
                     /locus_tag="PKO06_00395"
     CDS             complement(1663..2958)
                     /locus_tag="PKO06_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HNV68123.1"
                     /translation="MQAHLRRLTVAILVGLWFQMSAWAADNTTGATAFSAKVTGDIYG
                     QVKVIDDVDGDGKKDLVFGATDGMVHLYSAKGSEIYAGLWPKHTGGPILADVAVADLD
                     ADGQKEVIVGSYDGNVYGLNSWGKELWKVNTRGTIQLSAPEVADVEGDGKLSIFVGSR
                     AGTVSRIDAQGRLTWEVPMTTKVSAKVIASDLDGDGVKEIVCKDDSGKVSVLTKTGSV
                     FRGWPQNTAANLDWPFEVTAADLNGDGRREVITTTPDKKLNVWTTNGALLRQLKLTDG
                     AHSAPRLADMDGDGKAEFVFTQADGKVQVMKQDGTNLRGWPYDTKHSIYGAPQILDID
                     GDGRLDVVFTAWNPEGVGKQAGYILALSRDGKVLSGFPKHIGKTIAPLTFADLDDDGF
                     LEMIAAGGINYTDNQLHVIPTQARVQIRMAVLGTEVNFE"
     gene            complement(2981..3487)
                     /locus_tag="PKO06_00400"
     CDS             complement(2981..3487)
                     /locus_tag="PKO06_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68124.1"
                     /translation="MSRVPQLGRKGFIAVVAFILLVILGILGISYWSISRLSTDQILK
                     EAHRIKARSIAQAGIEKVLVHVMNQYRLRNYDLEYPGSKKYVQDRQEKEFKREVGDGY
                     YQVELIQAYEPPSTNAALRNRVYAKNRVPIGIYDVWRVVVVGVMPTTNVQARVETLVK
                     VIRQTVQY"
     gene            complement(3484..4119)
                     /locus_tag="PKO06_00405"
     CDS             complement(3484..4119)
                     /locus_tag="PKO06_00405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV68125.1"
                     /translation="MQHTSRTKRAFTLVEMSVTMLVVSIIGLILLSVFRSNIAAWKWG
                     SKHMEFNQKIQLAMKQVFTDIKRINPVVYADETGNYWFKGEKIGDLVPNLVDIIDLDK
                     EPANGGEELMMWHTSYVTPGEKTGVRLFLEEGALVRELILPNDTRRKSVVSNRVADLH
                     FQKNVDDPLEIKVSMVITDDRNPELKETLAFAVHLDTDLVYVRMAGAEVEP"
     gene            complement(4134..4610)
                     /locus_tag="PKO06_00410"
     CDS             complement(4134..4610)
                     /locus_tag="PKO06_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV68126.1"
                     /translation="MRISRSHHAFTLIEIMITVLIIGAGVVPVLSLFLSGSRTVEKGG
                     AILEVTIAAQNILDSARSDSFLWDHIPLKINIPDDKFPQFTLPEFFAKKYQASGTLEI
                     EVAPNHTILGTAEPEENLIQISVTINWIENQFPRTSRLATYRANTNSFDLKTSTRF"
     gene            complement(4623..5243)
                     /locus_tag="PKO06_00415"
     CDS             complement(4623..5243)
                     /locus_tag="PKO06_00415"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68127.1"
                     /translation="MKRAWRRRVSRSAVFLTLAIVCALTSFCTGCGKKKSPSENNQTG
                     GEYRIPVARQPGATTGPSVNLSGNVAFPWLQAGRSIDGKELSADQRKTAKAPAEAFNQ
                     KYASGLQHFESGELGKALEVFEEIVRSYPGSDEASMAEYRIAQIHFRNKANGRALETY
                     KRIVSDYPLSPVAENARAAITYLETFDTHEKAYVSPEADDERRRNR"
     gene            complement(5256..6605)
                     /gene="glmM"
                     /locus_tag="PKO06_00420"
     CDS             complement(5256..6605)
                     /gene="glmM"
                     /locus_tag="PKO06_00420"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV68128.1"
                     /translation="MARLFGTDGVRSVANTFPLTGDHAFRIAQAGTRHLLLNHKKHDR
                     PIFIGKDSRRSGDMLEHAVAAGVASMGAEVRLLGVVPTPAVAYITREHQGLGGIVISA
                     SHNPFQDNGIKIFGADGFKIPDAVEEAIEADLASNQMNKLPQGAGVGRITNDATSVQF
                     WLKNLSRVLADQSSPRRIKVAIDCANGALAPWAPKFFADHGFSVDAIGVSPDGVNINQ
                     DCGSTHIGALQKVMARERFDVGFAFDGDADRVIAMTSDGDVIDGDYILAIMARYLKQL
                     GRLTNNFLVTTVMSNLGLDLAMKKLDIKLLKTKVGDRFVLEEMQRSQAIVGGEQSGHV
                     ILYEYSTTGDGLLTACFLLRIIRDTGMSLKDLSQVMEKFPQVLVNIRVKNKNYDSVPE
                     ITSHIRQHEEKLSGRGRILVRPSGTENLVRVMAEGPDEREIRHIVDDLAKVISAHLC"
     gene            complement(6797..9850)
                     /locus_tag="PKO06_00425"
     CDS             complement(6797..9850)
                     /locus_tag="PKO06_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68129.1"
                     /translation="MTYVSGLFTGFVNMMFTLLNSFVTWLSFSSEIENMVDAVNHKID
                     EPPTRIGHRMSSHPGGFAKIVGKRRLNMAGAYIKISNDHAPQDAAERLTALKMLAEEA
                     LHAKTISMPLNTARVQIALMKKVVKSQGNKRRQMEAMSDFGLASYGNESTIRDFLKRN
                     SLIEVPELDAPLKAIDMGWDDHVHDFLSEGRKTPTQVILDAFVKGISRLTLVHSTIDQ
                     PQLIHEAISAGNILGIDVQIGIEFSVGKRNCRRHYMYIPPRVENSQEFFRFFYDHHEA
                     LEPFLKGLRLNSEKRQRTMLMAMERFNTTMLPKINAGYQPHAPGWVPPLAWEDLNAYI
                     VCGQVNRVHLGALLYTSMLRAFHARVLELKTQLLAAEERFRLGLYSEWEMKNVSSQYQ
                     NVRRTYETMSPEGLMSQYLDNREVLDYDSEFIDESPLLDTLRGLGGNILWIHPLELGL
                     KEAVTHLVQNCHRLTHIETLNLRDSVQRNPETINLFSRFVHLFNNQPMNEVRHYLDQN
                     DIQTLTMDEVEQQRHQMGERRLVPVCGSDSTGRDVAIPGMGFIQSSRIPEAVRTSFRE
                     HHFRLARPVAQLILSKGADPKSMSGAPEDQDIICMGKIGRPMHNSVGDEPAEHHFNVF
                     RFWHYLNSTVKNSVRVAIGFAAALYWMNYQFALSIGLFFACLWFFITGFRNFLVDMIA
                     SSGTDFRSWSLKNVNFDNLAQSLYWTGFSVPLLGLVKMQYDELWPLEKSGLLFETAKF
                     FFICFANGSYIMFHNRLRNFDRKVRRANFFRTMIAFPFATAFSSIGNAMGIPSIVQAK
                     FWSDFVAGIIEGSGKFRQRFVLRQRDLKELLPRLYSMDRDARLTAMLDILYIWARQPR
                     GKTCLAHLLLQSRSWWVMVKEWWYGRETPEEVRRIQGQYYLYYERICELYIDTGCLLN
                     MTQFILNRFSGRDAVMLTKLVGEELETFRTWLRHLRSHYDVKKPELVDVKEYLPPALQ
                     KEDTFEMPQSIDELKKTNLQSKPEPTVELAPDPVSDTATPEERKP"
     gene            complement(10174..11232)
                     /locus_tag="PKO06_00430"
     CDS             complement(10174..11232)
                     /locus_tag="PKO06_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68130.1"
                     /translation="MIFWRGMCAGLLALGLMCATPAAAVPKFSGDFSHRFWVKTETEN
                     SAGKVSTRRVSAERFRFMFEDKLKHAEWRFRIGMIDPTRSTGSIISLGREGDTVLGPH
                     HFQVIDCYWQWNLEDGWKVRLGRTGIPFFRYRSELVFDNDIFWDGLYVEKTLPWATKA
                     KTSAHAGAFTIKRKIHGEGDKLFVFGAMGQPKWGKVQAEWRVDRFQFDIDSKGWANTF
                     APSYRLVNLYGAMEWPSQVRVTADWSRNLAANAPVSMHSGGDAYNLTVLLGKMPRDFT
                     SQLIYQYFKVGPHAVPGSMVWYDKRVNIEGSAIVYKFRVSRRTEIELDFYNFRRFEAG
                     FATDRKYLRWETQLNHRF"
     gene            complement(11267..>11756)
                     /locus_tag="PKO06_00435"
     CDS             complement(11267..>11756)
                     /locus_tag="PKO06_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNV68131.1"
                     /translation="VPTWARGRPDAKPLINARSETIAEKVSFREAFRQRRCLIPADGF
                     YEWQRQGRGKIPYWFHRPARKHFAFAGLWEPGLEDNQTTCTIVTTTANAVVSGIHDRM
                     PVMLTEKNYDRWLYGQITEMAGLLVPFSVDDLLAERVSTRVNSVSVDDEMCVQPERNL
                     LE"
BASE COUNT         2541 a         3558 c         3065 g         2592 t
ORIGIN      
        1 tcgccggccc gatggtgtaa ttgagcgtca ccttctcgat gccttcttcc tggtcaatcc
       61 attccttgcg caggaagaac cacccctcga aggggcgagt cgaaggcaat tcgaccgcgg
      121 cgtcgttgga cgcttcaccc gcagcgggac ggtctgcggg aggcgagaga tcaagcgatg
      181 aattcacgtc actcatggtt gcgatcttcc tgcacttcgg tgcgctcgag ccggacaaaa
      241 acccggcccc gggggaatcg gtcagagtat atcataaacc cgcaggcata ccaatggaaa
      301 acatgcgccc gccaaacagc ccctgatcac tttttgagag tgttcttggc cgcgacgata
      361 tcgcgaatca tggtttcgga atcttgagcc agctttgacc caggagtcag gcgcagtacc
      421 tggttaaacg cttcaatagc ccgatcgaat gcttcgacct gcccattcaa gcctttgtcc
      481 gttaaagcca gacccaggtg ataatggaac ttcggctcgt tcgcattctc tttgatcgca
      541 gccgtgaact tctcgatggc ggcctcgatc tggcctctct tgtacaaatc atagccatcg
      601 ttatcgagca tctcagagcc gcttgtggaa gttcccgccg tgccactgcc gaccgccgcc
      661 gccgcgctgg cattgctggc tgccatctgc tgggccagca actgcgcctg tttcgccgct
      721 tcttccgcct ctttctggcg tttgatctcc gcgagacggc gctcctcttc cgccttccag
      781 tcgtcgagtt ccttcaagac cttgcgcacc agcggctcat gtgagcctgg gttggcccgc
      841 agcggctcga acaactcgag ggttcgatcg cgcatgccgt tgaggtaggc aatcatgccg
      901 agaaagaacc gcgcctccgg gtcttcctta ttgctttcca gacgcggatt gaaataagtc
      961 agtccctctt caagcagctt gcggtcatcg taagccccga tcgaccgatc ggcgagaatg
     1021 agttcgaagt atttctccag agccaccggt agtccgtgcg aaacccagag atcgaagact
     1081 tccgtcaggg gtccctcgtc tttcgtgaag gattgaagtt cgggagaggt cgtcgccgga
     1141 tcgaggctca gcggtttttc cgcttcgtct gccagcggat ctttggctgc caccttcacc
     1201 cgggtttccg gcaccaccag gccgtacggc cgctggcgcg ttccagaccc cagaagcgtc
     1261 agcgcttcat acgtgacgag gtccgcttcc ttggccttca catgcccgcg gtcagagccc
     1321 aacagggtgg tcagttcttt cacggcggcg tgatccgagt tcacctggcg agctcgcgac
     1381 aacatgtcct gggctttccg cagttctttg cgatcagcat agacacgcac cagacagacc
     1441 atggcatcca gatttcgcgg atcgagcaac agcacgtttt caaactcggt ctgtgctccc
     1501 agcaggtcac gcttctgata gcaggccttg ccggccgtga gaaactcgtt gatgtcttcc
     1561 gacatctggg ccatcgctgt acaagcgaga aacagggcgg ccatggcccc caagagtccg
     1621 cgccacctta tcgagaacct gccactcatg ctcggtactg cctcactcga agttgacttc
     1681 cgtacccaat acggccatgc gaatctgcac ccgcgcctgc gttggaatga cgtggagctg
     1741 gttgtctgta tagttgatgc cacctgccgc aatcatttcg agaaatccgt cgtcatccag
     1801 atcagcaaac gtcaacgggg caatcgtctt gccgatgtgc ttgggaaaac ccgacagcac
     1861 cttcccgtct cgactcaacg cgagaatata ccctgcctgc ttgccgaccc cctctggatt
     1921 ccaggcggtg aacaccacgt caagtcgacc gtcgccatcg atatcgagaa tctgtggcgc
     1981 gccatagatc gaatgtttcg tgtcatacgg ccagccacgc aggttcgtac catcctgttt
     2041 catgacctgc accttgccat ccgcctgcgt gaacacgaac tcggctttgc cgtcgccgtc
     2101 catgtccgcg agccgcgggg ccgaatgcgc gccgtctgtc agtttgagct gtctcagcaa
     2161 cgccccgttc gtggtccaga cgttcagctt cttatccggt gtcgtggtga tgacttcacg
     2221 ccgcccatca ccattcagat ccgcggcggt gacttcaaac ggccaatcga ggtttgcggc
     2281 ggtgttttgc ggccagcctc gaaacactga cccggtcttg gtcaagaccg atacctttcc
     2341 ggaatcgtcc ttgcagacga tctccttcac gccgtcgcca tcaaggtctg aggcaatcac
     2401 ttttgccgat accttggtgg tcatcggaac ctcccaggtc aggcgaccct gcgcatcaat
     2461 ccgggacacc gtgccagcgc gcgaaccgac gaaaatggaa agtttgccat ctccctccac
     2521 atcagccact tcgggagcag aaagttgaat cgttccgcgc gtgttcacct tccacagctc
     2581 ttttccccag gagttcaagc cgtagacatt gccgtcatac gatccgacga tcacttcttt
     2641 ctgcccgtcg gcatcgaggt cggccaccgc gacatcggca agaatggggc cgccggtatg
     2701 tttgggccac aagccggcat agatctcgct gcccttcgcc gaatacagat gtaccattcc
     2761 gtccgtggcg ccgaagacca gatctttctt cccgtcgcca tcgacgtcgt cgatcacttt
     2821 cacctgaccg taaatgtcgc ccgtcacctt ggccgaaaac gcggttgccc cggtggtatt
     2881 gtccgccgcc cacgcactca tctggaacca caaaccgacc aggatcgcta ctgtcagtct
     2941 tcgcagatgc gcttgcatga atttcctcca gacttgccga ctagtactgt accgtctggc
     3001 gaatgacctt caccagcgtt tcaacccgtg cttgaacatt ggtggtcggc atcaccccaa
     3061 ccaccacgac tctccagaca tcgtagatgc ctatgggaac tcgattcttc gcatacaccc
     3121 ggttgcgcag cgcggcgttg gtcgacggcg gttcataggc ctggatcagt tcgacctgat
     3181 agtagccgtc gcccacttcg cgcttgaact ctttttcctg acgatcctgt acgtatttct
     3241 tcgatcccgg gtattccaga tcatagttcc tcaaccggta ctgattcatg acgtgcacca
     3301 gcactttctc gataccggcc tgagcgatcg agcgcgcctt gatgcgatgc gcttctttca
     3361 aaatctgatc cgtgctcagg cgagagatcg accaatagga gattcccaga attccgagga
     3421 tgaccagtag aatgaacgcg acgaccgcaa tgaatccctt ccgtcccagt tgtggcaccc
     3481 ggctcacggc tccacctccg cgccggccat acgcacatac accagatcgg tatcgagatg
     3541 caccgcaaag gccagcgttt ctttcagttc cgggttccgg tcatcggtga tgaccatgct
     3601 gaccttgatc tcgagcggat catcgacatt cttctgaaag tgcagatcgg ccactcgatt
     3661 cgagacaacc gacttgcgac gcgtgtcgtt cggcagaatc agttcccgta ccagcgcccc
     3721 ttcttccaga aacagacgaa caccggtttt ctcacctggt gtcacatatg acgtatgcca
     3781 catcatcagt tcttcgccac cgttggccgg ctctttgtcg agatcgatga tatccaccag
     3841 gttcggcacc agatcgccga tcttctcgcc cttgaaccag taattcccgg tttcatccgc
     3901 atacaccacc gggttgatac gcttgatgtc ggtaaagacc tgtttcatgg ccagctgaat
     3961 tttctggttg aattccatat gcttggagcc ccacttccag gccgcaatat tgctgcgaaa
     4021 gaccgaaaga agaatcaacc cgatgatcga aactaccagc atggtcacgc tcatctcaac
     4081 cagcgtgaac gcgcgttttg tccgtgacgt gtgttgcatc gcagccggcc ccctcaaaat
     4141 cgcgtcgacg ttttcaggtc gaagctgttg gtgttcgctc gataggtggc cagcctgctg
     4201 gttcgcggaa actgattctc gatccagttg atggtcacgg aaatctgaat gaggttctct
     4261 tcgggctcgg ccgtgcccag tatggtgtga ttcggcgcga cctcgatctc gagcgtgccc
     4321 gaggcctggt actttttcgc aaagaattcg ggcagggtga actgcggaaa cttgtcgtcc
     4381 ggaatgttga ttttcagcgg aatgtggtcc cacaggaacg agtcactgcg cgccgaatcg
     4441 agaatgttct gggcggcgat cgtcacttcg agaatggccc ctcccttctc gaccgtgcgt
     4501 gaaccggaca gaaaaaggct gagaaccgga accactcccg caccgatgat gaggaccgtg
     4561 atcatgattt cgatcagggt gaacgcgtgg tgactgcgag agatcctcat tatcagggcc
     4621 tcctagcgat tacgtcgccg ctcatcgtct gcttcggggg atacatacgc tttctcgtgc
     4681 gtatcgaacg tctcgagata ggtgatggcc gcccgcgcgt tctccgctac cggagacaat
     4741 ggataatcgc tgacaatacg cttgtatgtc tcaagagctc ttccattcgc cttgttgcga
     4801 aaatgaatct gcgcgatgcg atactcggcc atggaagctt catcggaacc cggatacgag
     4861 cgaacgatct cctcgaagac ctccagggcc ttgcccagtt ctcccgactc gaaatgctgc
     4921 aggccagacg catacttctg gttaaaagcc tcggctgggg ctttcgcggt cttgcgctgg
     4981 tctgccgaca gctctttgcc atcaatactt cgaccagcct gcagccacgg aaaggccaca
     5041 ttgcccgaca gattcaccga cggccccgtc gtcgcacccg gctgacgcgc gactggaatg
     5101 cgatattcgc cacctgtttg attattttct gatggggatt ttttcttgcc gcaaccagta
     5161 caaaatgacg tcaaggcaca gacaatcgcc agtgtcagaa acacggcaga ccggctcaca
     5221 cgtcgccgcc atgccctttt catcgtgtat accacctaac agagatgagc agaaatcact
     5281 ttggcgaggt cgtcgacgat atgacgaatc tcacgttcat ccggaccttc cgccatgacc
     5341 cgcaccaggt tttccgtccc cgatggtcgt accagaatac gaccgcgccc ggaaagcttc
     5401 tcttcgtgtt gtcgaatgtg actcgtgatt tccggaacac tatcgtaatt cttgttcttg
     5461 acccggatgt tcaccagcac ctgcgggaac ttttccatga cctgactcag gtctttcagt
     5521 gacatgccgg tatcacgaat gatgcgcagc aggaaacacg cggtcagcaa gccatctccg
     5581 gtggttgaat actcgtacaa gatcacatgg cccgattgct caccaccgac gatggcctgc
     5641 gagcgctgca tttcctcgag gacgaaccgg tcgccgacct tggtcttcaa gagcttgatg
     5701 tcgagcttct tcatggccag atcgaggccg agattgctca tcaccgtcgt caccagaaaa
     5761 ttgttggtca gacgtcccag ttgcttcaag taccgggcca tgatcgccag aatgtagtcg
     5821 ccatcaatga catcgccatc cgaagtcatg gcaatcacgc gatcggcgtc gccatcgaag
     5881 gcgaacccga cgtcgaaccg ctcgcgcgcc atcactttct gcaacgcgcc gatatgcgtc
     5941 gagccacaat cctggttgat attcaccccg tccggggaca caccgatcgc atcgacactg
     6001 aagccgtgat cagcaaagaa cttgggggcc cagggagcca acgcgccgtt ggcgcagtcg
     6061 atggcgactt tgatgcgacg cggtgacgac tggtcagcga gcacgcgtga caggttcttc
     6121 agccagaact gcaccgacgt cgcatcgttg gtgatacggc caactcccgc gccctgcggt
     6181 agtttgttca tctgattgga cgccagatcc gcttcgatcg cttcttcgac ggcatcggga
     6241 atcttgaatc cgtctgcgcc gaagatcttg atgccattat cttgaaaggg gttgtgtgag
     6301 gcactgatga caatgccgcc gagcccctga tgttctcggg tgatataagc aaccgccgga
     6361 gtcggtacca ctcccaacag gcgcacctcg gcccccatcg atgcgacacc ggccgcgacc
     6421 gcatgttcga gcatatcgcc cgagcgccgc gagtctttgc cgatgaagat cggacgatcg
     6481 tgtttcttgt gattcagcaa caggtggcgc gtgccggcct gcgcaatacg aaaggcatgg
     6541 tcgccagtga gaggaaaggt gttggcgaca gagcgcacgc catcggttcc aaagagtcga
     6601 gccatcggtg agagtgacct ccgggacgga ataagaacga caaactcagt ataccatcac
     6661 cagcaacgga aaaacactat cgtagccggg aatcagagcc gtccgttctt gcaggaccat
     6721 ttcgaaatca cggtcaggcg aacccgatgc gtgcgtcgaa agccgttctg gggagttcgt
     6781 ggcccgctgc gctccgttac ggtttgcgct cttccggcgt cgccgtgtct gagaccgggt
     6841 ccggggccag ttccaccgtc ggctccggct tcgactgcag attcgtcttc ttcaattcat
     6901 ctatgctctg cggcatttcg aacgtatcct ctttctgtaa cgccgggggg agatattctt
     6961 tcacatcgac cagttccggt ttcttcacat cataatgaga acgcagatgc cgcaaccacg
     7021 tccgaaaggt ctcgagctcc tcgccgacca gcttcgtcaa catgacggca tcccgaccgg
     7081 aaaagcgatt cagaatgaac tgggtcatgt tcaacagaca tccggtatcg atgtacaact
     7141 cacagatgcg ctcatagtag aggtaatact ggccctgaat ccgtctgact tcttcgggcg
     7201 tctctctccc ataccaccac tctttcacca tcacccacca cgaccggctc tgaagcagca
     7261 ggtgcgccag gcaggttttg ccacgcggtt gtcgggccca gatgtacagg atatcgagca
     7321 tcgcggtcaa tcgcgcatcg cgatccatgg aatagagtcg cggcagcagt tccttcaggt
     7381 cgcgttgtcg cagtacgaac ctctgacgga atttaccact cccctcaatg atcccggcaa
     7441 cgaaatctga ccagaacttg gcttgtacaa tcgacggaat ccccatggcg tttccgatcg
     7501 acgaaaacgc cgtggcaaaa gggaaggcga tcatggtgcg gaagaagttc gcccgcctga
     7561 ccttgcgatc gaagtttcgc agccggttgt gaaacatgat gtaggaaccg ttcgcaaaac
     7621 agataaagaa gaacttcgcc gtctcgaata gcagtcccga tttctcgagc ggccacagtt
     7681 cgtcatactg catcttcacc agacccagca ggggcacgct gaagccggtc caatacaagg
     7741 attgcgccag gttgtcgaaa ttgacgttct tcagcgacca tgagcgaaag tccgtgcccg
     7801 aagacgcgat catgtcgacc agaaaattcc ggaacccggt gatgaaaaac cagagacagg
     7861 caaagaacag accgatcgac agggcaaact gatagttcat ccagtagagg gccgccgcaa
     7921 agccgatcgc cacgcgaacc gagttcttca ccgtcgagtt caggtaatgc cagaagcgaa
     7981 acacgttgaa atggtgttcc gccggctcgt cgccgacgct gttgtgcatc gggcggccga
     8041 tcttgcccat gcagatgata tcctgatctt ccggagcacc cgacatgctc ttggggtcgg
     8101 cccccttgct gagaatcagc tgcgccaccg gtcgggccag tcggaaatga tgctcacgga
     8161 acgaggtgcg caccgcctcg ggaatacgac tggactggat gaaacccatg ccgggaatgg
     8221 ccacgtcacg gccggtcgaa tcgctgccgc agacgggaac caggcgacgc tcgcccatct
     8281 gatgtcgctg ctgctcgacc tcgtccatcg tgagggtctg aatgtcgttc tgatcgagat
     8341 aatgacgcac ttcattcatc ggctggttat tgaacaaatg gacgaagcga ctgaacagat
     8401 tgatggtttc cgggttccgc tgcacactgt cacgcagatt cagcgtttcg atatgcgtca
     8461 gtcggtggca attctgtacc agatgggtca cggcttcttt cagtcccagt tcgagcgggt
     8521 ggatccacag aatgttcccg ccgagacctc gcagcgtatc gagcagaggc gattcgtcga
     8581 tgaactcgct gtcgtaatca agcacttcac gattgtccag atactgcgac atcaggcctt
     8641 cgggagacat ggtctcatac gtgcgccgca cgttctgata ctggctcgaa acgttcttca
     8701 tctcccattc gctgtacagg ccgagtcgaa atcgttcttc ggctgccagc agctgcgttt
     8761 tcagctcgag cactcgcgcg tgaaacgcgc gcagcatcga ggtatacagc agcgccccga
     8821 gatgcacgcg attcacctgc ccgcaaacga tgtaggcatt caggtcttcc cacgccagag
     8881 gtggtaccca cccgggggca tgcggctggt acccggcgtt gatcttcggc aacatggttg
     8941 tattgaagcg ctccatggcc atcagcatcg tccgctggcg tttttcggag ttcaggcgca
     9001 gccccttcag aaaaggctcg agtgcctcat gatggtcata gaaaaatcgg aaaaactcct
     9061 ggctgttctc gacccgtggc ggaatgtaca tgtaatgccg gcggcagttc cgcttcccga
     9121 cgctgaattc gatcccgatt tgcacgtcaa tgccgagaat atttccggca ctgatcgcct
     9181 catgaatcaa ttgcggctga tcgatcgtcg agtgcacgag agtcaaccgt gaaatgcctt
     9241 tcacgaaggc atccagaatc acctgcgttg gggttttccg accttcagac agaaagtcgt
     9301 gaacgtgatc gtcccagccc atgtcgatcg ccttcagcgg ggcatcaagc tctggaacct
     9361 cgatcaaact attccgtttg agaaagtcac gaatggtcga ttcgtttcca tacgaggcca
     9421 ggccgaaatc agacatcgcc tccatctgcc gccgcttgtt gccctgcgac ttcacaacct
     9481 tcttcatcaa ggcaatctgc actcgcgccg tgttcagcgg catgctgatc gtcttggcat
     9541 gcagggcctc ttccgccagc attttcagcg ccgtcaaccg ctcagccgca tcctgcggcg
     9601 catgatcgtt cgaaatcttg atatacgcgc cggccatgtt cagacgccgc ttgccgacga
     9661 tcttcgcaaa gccgcccgga tgcgaactca tgcgatggcc gatgcgcgtc ggcggttcat
     9721 caatcttatg gttgaccgca tcaaccatat tctcgatctc agaactgaag ctcagccagg
     9781 tgacaaagct gttcaggagc gtaaacatca tgttgacgaa ccccgtgaac aacccggaaa
     9841 catacgtcat gcaacgcact cccgcgacag ttgttggtcg tggtctcaca aaatcgaccg
     9901 gacagaataa gatagcacac gtcgcccgga agacaaacga gatttcacac cggcgttccg
     9961 tgcacggaaa tcgcttcttc agatcgtcag cgcatctgac gctgacccaa cgcgtgatcg
    10021 atggtatggt cctgcacgtt tcagaggccc ggcggaaacc cggaaacttc agcgctcttc
    10081 tgccgtacca ctcacgtgaa ccggctcgct gaaacaaaac gggcggtatg cggaagagat
    10141 ccgataccgc ccgcgcagga cgctgtcaga caatcagaag cgatgattca gctgggtctc
    10201 ccagcgcagg tatttgcggt cggtggcaaa acccgcctca aaacgacgaa agttgtagaa
    10261 atcgagttcg atctcggttc gacgactcac ccgaaacttg tagacaattg ccgaaccttc
    10321 gatgttcacc cgcttatcat accagaccat ggaaccagga accgcatgcg gtcccacttt
    10381 gaagtattgg taaatgagtt ggctggtaaa gtcacgcggc atcttcccca gcagaaccgt
    10441 caagttgtaa gcgtctccac ccgaatgcat cgatacaggc gcattggccg ccaggttacg
    10501 agaccagtct gcggtcacac gcacttgtga cggccattcc attgcgccat agagattcac
    10561 gagccgatac gaaggggcga aggtattcgc ccatcccttg ctgtcaatat cgaattgaaa
    10621 gcggtcaaca cgccactcgg cttgtacctt cccccatttt ggctgcccca tggccccaaa
    10681 cacaaacagc ttgtcccctt cgccatgaat tttgcgtttg atggtaaaag cacccgcgtg
    10741 tgccgaggtc ttggccttcg tggcccaggg cagggtcttc tctacgtaca agccatccca
    10801 gaagatatcg ttgtcaaaaa ccagttccga acgatagcga aagaacggaa ttccggtgcg
    10861 acccagacga actttccacc cgtcttccag gttccactgc cagtaacagt cgatgacctg
    10921 aaagtgatga ggcccgagaa cggtatcccc ctcacgtccg agactgatga ttgagccggt
    10981 agagcgcgtc gggtcgatca tgccgatacg gaatcgccat tccgcgtgtt tgagcttgtc
    11041 ttcgaacata aaacgaaacc gctcagccga gacacgacgt gtcgaaacct tcccggcgct
    11101 gttttctgtt tcggttttca cccagaagcg atgcgaaaaa tcgccggaga acttgggaac
    11161 cgccgccgcc ggagttgcgc acatcaatcc aagcgccagc agaccagcgc acattcctct
    11221 ccagaagatc atcgttgtca ctcctcctct tgttgtgtcg ggtcagtcac tctagcagat
    11281 tccgttcggg ctgaacacac atttcatcat cgacggacac cgaatttacc ctagtcgaca
    11341 cccgctctgc cagcaagtca tcgaccgaaa acggcaccag cagccctgcc atctccgtta
    11401 tctgcccgta cagccagcga tcgtagttct tctccgtcag catcaccggc atgcggtcat
    11461 ggattccgga gacgacggcg ttggcggttg ttgtcacaat agtacaagtt gtctggttgt
    11521 cttcaaggcc tggctcccat aatccagcaa aagcaaaatg cttgcgtgcc ggacgatgaa
    11581 accaataggg aattttcccg cgcccctgtc tctgccactc gtaaaacccg tctgctggaa
    11641 tcaaacaacg ccgttgccga aaagcctctc gaaatgacac tttctcggcg atggtctctg
    11701 accgtgcgtt aatcagaggt ttcgcatcag gtcgcccgcg agcccatgtc gggacc
//
LOCUS       DAORHD010000008        11489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_4809_length_11489_cov_4.183750, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000008 DAORHD010000000
VERSION     DAORHD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4809_length_11489_cov_4.183750"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4453
                     /locus_tag="PKO06_00440"
     CDS             <1..4453
                     /locus_tag="PKO06_00440"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNV68132.1"
                     /translation="PDKTARLAIPLTFRTNADKGGSFLVQLCDPEEKTAASAARLLQI
                     TDLSITYKLGTTNALFWVTSLEKGSPVAGAAVLAIDRHDHRFLLGRTNAEGLLFVPAS
                     STYPAVTTTGEGPRLAPQTFDPQAVVLVVAATPTDSSFVQVMANRFKPFGVETAAPGQ
                     AESRTRTGHIFTERGVYRPGETVHFKLAMRRFVDFAVRAPEDEKVEVTITNPKDDIIY
                     SEDLVLNSFGTASDSLPLKDFAPLGEYTVKVTYKALDQEKAEPVTVANTTFQVQKFEP
                     PRHYVTIATERKTRPDNSVVTRPTQEEYLEAKIESKYYTGGFVKNAKVRWTANLVPVT
                     PNIPGFETYRFGNHTTGNTLVESGEALLDQDGALSVALPLDRSLINGLFGLEISATVL
                     DVDGRPATKVDTYQPKAPFRVGMTKLPGTILPGDVISVGAIAVDPQNRRITSGAIRFE
                     VFKKRYYYVQKRDEDGNLFYRWESGWTRMTSSEQQIRDGEALFEVGFDDYWSELQLQM
                     VYTTPEGEFMCRVPVEIGYVSDSDEDEDETNRRRKSDHQVLLLLSQSEAAAGEKVMVS
                     FNLPRPATHALVTCEQQNLLDYKVIAVKGRYGTFDVVMKPESRPNVFVTMTAAAGRNG
                     LPVYRSQVDAFPPSVYYGVDRVRVKNVISSLKVEIQPTEANLKGRPGEPRELEFIVTD
                     AAGKPAVCEMAVCVVDEAVLSLTGFVTPLLHRLAEFTTPLTVFTGDLRLSLITQELYK
                     LFRTNPLTGGDSGGGGLASDLAMRRDFRPVAYWNPALQPDAQGKARIRFTLPDSTTAY
                     RVYVVALDRGDAFASVDRQMVVTKEFYVQPGMPRFLTVGDKAVFPLSACNKTEDALEA
                     DLKVEGRNVLVKLSQPKASMKPMTNTPVPVEVEADTHAPEALLTFRGTAGAFNDGIEL
                     PLPLQPRRLAINRARIGHFTDKAEIDAEIPAAVSLIPENERRGVLTAIVALSTTEWTK
                     VAPGLRYLLQYPYGCIEQTSSGIIPLAALRRMAAQNLIPGVTKETVDKFLKAGVLRVL
                     RMQRSSGGFAYWPEQSEVTWWGTLYATFALTAAQQAKLEDPDAAFEVPESALEKALSY
                     IAQRVLKEKDSSDWHYYGVYELAAVNLALNNKLTAADLDRLLAEFDKRSEEGKAFLIW
                     AAAASKHLTPARMKDLLKKLNPKLGPSSTTWYDSGTRELAAALAAQMQADPSSRKADD
                     YAGSLLKSVNREGYWGSTAHTGWCLYALSQYLTKKMLGKGEEVPVEVRQPGRDPVKVR
                     VGQNSSEVELDAEALLKNGKVQLTTAGKKLVNWTLMVTYPDTATSAEDLNQGFLVEKK
                     ITNLNGSNEFRVGDIVKVSIEFADTPSQSQNWRNYRNYEYLALEDPLPAGFMAINTAL
                     KNDRRPVTTNEDGEESSSDDSESEEDWAPWEDGAYLFRPDHSEMHPEKVVAFRDRLWS
                     NRFRFTYFARAICEGEFMLRPTRVSLMYDPETYGLSQSQTVKVLPAK"
     gene            complement(4594..4875)
                     /locus_tag="PKO06_00445"
     CDS             complement(4594..4875)
                     /locus_tag="PKO06_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68133.1"
                     /translation="MKKHHLLFGILLSCLLTTGCEISSSRQLEEEIAKLDAEIKGLHA
                     KRSELLQLHDEIADLKKRIDETQRKKDQFLQDHPGLEEYAKKEWAKESK"
     gene            complement(4872..5141)
                     /locus_tag="PKO06_00450"
     CDS             complement(4872..5141)
                     /locus_tag="PKO06_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68134.1"
                     /translation="MMNSLRRRFAVVLLGALLLPGFIGCMDFSGKLRRQREKLLEERD
                     NADREVRRHVHLFNERDQVRQELSNLEAQLELYEKYQKQREAEKK"
     gene            complement(5131..5934)
                     /locus_tag="PKO06_00455"
     CDS             complement(5131..5934)
                     /locus_tag="PKO06_00455"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV68135.1"
                     /translation="MKRLTRSLRNPDGGIRPTDLEIVFTDEDFVVVNKPSGLLSIPDR
                     FRKHLLNLHDLLSQRFAKVHPVNRLDRDTSGLVVFARNSEANVDLSDRFARQEIQKTY
                     LALVKGEMPNDEGVIDAPIGEHPDQPGLMKIDRKTGKPAVTEYRVVERFKGLTLIEAK
                     PRTGRQHQIRVHLAHIGFPLAVDSDYGGAGSILLSAFKKSYKPGKEGESPLIGRLSLH
                     SWKLSFHHFRRNDALELIAPLPRDFEVALKQLRKYAAKTMSTQEIPDDE"
     gene            complement(5931..6782)
                     /locus_tag="PKO06_00460"
     CDS             complement(5931..6782)
                     /locus_tag="PKO06_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNV68136.1"
                     /translation="MRHSQLCRYRLFLLCLCIVIILALSQRLNAQDAELKIGVLYNHS
                     QSLAMARGRDTLKSYSRAIIENGGRAIALLVTDEASTIMRKMKMLDGLLVPGGDDIDP
                     KLYGEEPHPKLETVERGLDRFELEVLKMADRRQMPVLGICKGLQLMNVHRGGTMIQDI
                     PSETQRSGRFQHRRPAGAEAKLATHAVNIESDSLLWQALQARRLLVPSVHHQAIEKLG
                     EHLRVTARADDRIIEAIEATNHPFFVGVQFHPERALPTDPRFHGLFQALLTAAEGYRL
                     TRKESHP"
     gene            6862..7287
                     /locus_tag="PKO06_00465"
     CDS             6862..7287
                     /locus_tag="PKO06_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010629481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV68137.1"
                     /translation="MVQPPTVTGINHLTFAVRDLDRSLAFYVEVLGLCLVARWQGGAY
                     LRAGAVWIALFVDDAVRSAPHPDYTHTAFGVSSAGFAELAARVLGCGAEVWQQNQSEG
                     ASLYVLDPDGHRLELHVGDLEDRVRSLRANPPPGLQVFV"
     gene            7284..9884
                     /gene="pbpC"
                     /locus_tag="PKO06_00470"
     CDS             7284..9884
                     /gene="pbpC"
                     /locus_tag="PKO06_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNV68138.1"
                     /translation="MIETRRERWRRLGWRLAKLALLAGVLAWMLWEFALAFLIPDYSA
                     RVRAAVADGGVLLDRDGRMLRLFPNADDEFLLSSPPASFPELLCQAVLAAEDRRFYEH
                     AGFDPWAIVRAFWLNLRHQRVISGASTITQQVIRMVRPASRTILTKISELLLAARLER
                     SLNKTEILTAYLNLAPMGGNVRGAPLAARVFWGKSLEQLTCAEAAVLAGIPQAPSRLD
                     PNRWKGHRLLKKRRNWILQSMRELGFITVEEEKQGCQAPVWANARRFPFLAPHFCDWV
                     VAQIGRLQGWITTTVDLDVQQMVEETLQAHEARLRRSGAGQASVLVVDTRSMQMLAMV
                     GSLRFGPLAGGYNNGCVSPRSGGSVLKPFLYALAFENGFHPCSVVPDTRQHFQTPQGD
                     YLPLNADRRAYGPVTIRQALGNSLNISAVKMLNVVGVRTLYQLLVDLELLKPDPSGPD
                     RYGLGLAIGNPEIRMIDLIRAYGAFAHRGALKPITYLASSTAPLRSPLLTQESAFLIL
                     DILSDPTARLLTFGNPRYFSFDGPVALKTGTSTDYRDCWLIAVTPRYIIGLWAGNFDG
                     RPTFGLSGASACGPVLRDLLQNLPGALSEPWFRVPPGIREIPICGISGQTPTPFCDRL
                     SRELVSRDSEVPQTCTFHTSNAATHELPMEYADWLQQRRLTLQDDPFTLSHGVATPDP
                     LRPSSLMPGTAQGGVRVRAVQPDVLSWWPFPFNAMVSQYRSLGDPGSQRDVSFGWLAS
                     APLWLSARAVSETAVASFASPVPEPSRRITVSQGLDRSPLAQVGRITILQPHDGDRFV
                     LVPDQENVTRFRAVPEEPLPEIIWLLNGRELARTPPPYEFFWTMEKGRHEIAAIGPGE
                     QGSSIMIEVE"
     gene            complement(9887..10858)
                     /locus_tag="PKO06_00475"
     CDS             complement(9887..10858)
                     /locus_tag="PKO06_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV68139.1"
                     /translation="MPTMTWVRRFLGTACVSALLFAASDLPLFAAQRLITGAAVRVRA
                     EPTTKSAEVLKLHFGDVLEQLEKSSKPETIGKKQDHWYKLKLPSGSSGWVFGGFTIDF
                     DDSRREALYRRLVEERLAQDSQNALDWLELFEFLDRVNPTITSTDDKPFFELGHLRAL
                     QKYLDFCQVKNVEELEAVPSVRGLLSQLIWNEPAAACLVNRDLFWDLYQRYRDHAFAD
                     ELAWAAASQDLPGETEGYPPAVVVNAWEKLGRYLEAKPQGKYAEEAVARLGEWLTFSD
                     ADLKQVEGDEIKRMKGDLEKLKKVVQFTLAKSRDAVMNKLTDLLMSL"
     gene            complement(10982..>11489)
                     /locus_tag="PKO06_00480"
     CDS             complement(10982..>11489)
                     /locus_tag="PKO06_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNV68140.1"
                     /translation="GGNYAASLLAGRVAREKGCAQVLWLDGVHHRYVEEVGAMNIFFV
                     FNGDTLVTPKLNGSILSGITRDSVLVLGKHLGLKTEERELSIDEVVGGITTGSITEIF
                     GSGTAASISPVGNLKYREKDYVVGHRRVGPITQKLFDMLLGIQYGEISNPFGWTRPVE
                     LNNKAEHA"
BASE COUNT         2255 a         3321 c         3424 g         2489 t
ORIGIN      
        1 gcccgacaag accgcacgcc ttgcgattcc cctgaccttt agaaccaacg ctgataaggg
       61 tggttccttc ctcgtacaac tgtgtgaccc ggaggagaaa accgcggctt cggcagctcg
      121 tctgctgcag atcaccgacc tgtcgatcac ctacaaactg ggaacaacga acgctctgtt
      181 ttgggtgacc tctctcgaaa agggaagtcc ggtggccggc gccgccgtgc tggcgattga
      241 tcgtcacgat catcgatttc tgctcggtcg cacaaatgct gaagggctgc tgttcgtgcc
      301 cgcgtcttca acgtatccgg ccgtgacgac gaccggagaa gggccgcgtc tggcaccgca
      361 gacgttcgat cctcaggcgg tcgtgctggt ggttgccgcc acgcctactg actcgagctt
      421 tgttcaggtc atggccaatc gtttcaagcc gtttggcgtg gaaacggccg ctcccggaca
      481 ggccgaatcg cgcactcgta caggtcacat ctttacagaa cgcggcgtct atcgacctgg
      541 cgagacagtg cattttaaac tggcgatgcg ccggtttgtc gattttgccg tgcgggctcc
      601 agaggacgaa aaagtggaag tgacgatcac caatccgaaa gatgacatca tctactcgga
      661 agacctggtg ttgaacagct tcggaaccgc ttcggattcg ctgccgctca aggatttcgc
      721 gccactcggc gaatacacgg tgaaagtgac ctacaaggct ctggatcagg agaaggccga
      781 gccggtgacg gtcgcgaata cgacgttcca ggtgcagaaa tttgaaccac cgcgtcacta
      841 tgtgacgatc gcgaccgaac gcaagacgcg cccggacaat tccgttgtca cccgcccgac
      901 gcaggaagaa tatctcgagg cgaagatcga gtccaaatac tacaccggcg gtttcgtgaa
      961 gaatgccaag gtgcgctgga ccgcgaatct ggtgccggtg acaccgaaca tcccgggttt
     1021 tgaaacctac cgtttcggaa atcacaccac gggaaacacg ctcgttgaaa gcggagaagc
     1081 tctgctcgac caggacggag ccctcagtgt tgcgttgccg ctcgatcggt ctctgatcaa
     1141 tgggctcttc ggtctcgaga tctcggcgac ggttctcgac gtcgatggcc gaccggcgac
     1201 caaggtcgat acgtatcagc cgaaagcccc cttccgggtg ggcatgacca agttgccggg
     1261 aacgatcctg cccggtgatg tcatctcggt gggcgctatt gcggtcgatc cgcagaatcg
     1321 ccgcatcacc agcggagcga ttcgcttcga agtcttcaag aaacgctact actacgtgca
     1381 gaaacgtgat gaagacggga atctctttta ccgctgggaa agcggctgga cgagaatgac
     1441 cagcagcgaa cagcagattc gcgacggcga ggctctcttc gaggtcggct tcgacgatta
     1501 ctggagcgag ttgcagctgc agatggtcta cacgaccccg gaaggggagt tcatgtgtcg
     1561 cgttcccgtc gagattggct acgtttccga ctcggacgaa gatgaggatg aaaccaatcg
     1621 tcgacggaag agcgaccacc aggtgcttct gctgttgtcg caatctgaag cggcggcggg
     1681 tgagaaggtc atggtcagct tcaacctgcc gcgaccggcg acgcatgccc tggtgacttg
     1741 cgagcagcag aatctgctcg attacaaggt gatcgcggtc aagggacgct acggaacctt
     1801 cgatgtcgtc atgaagcctg agtcacgacc gaatgtcttc gtcaccatga ccgcggctgc
     1861 cggccgcaac gggttgccgg tctaccgcag ccaggtcgat gccttcccgc ccagcgtgta
     1921 ttacggtgtc gatcgagtgc gcgtgaagaa cgtcatcagt tctctcaagg tggagataca
     1981 gccgacggaa gccaatctga aggggcgccc gggtgagccg cgtgagctgg aattcatcgt
     2041 caccgatgcc gccggcaaac ctgcagtgtg tgagatggcg gtctgtgtcg tcgacgaggc
     2101 cgtcctgtcg ctgaccgggt ttgtgactcc gcttctgcat cgtctggccg aattcaccac
     2161 gccgttgacc gtgttcacgg gtgatctgcg gctgtcgctg atcacgcagg aattgtacaa
     2221 gctgtttcgc acgaatcctc tcaccggtgg tgattccgga ggtggtggct tggcatcaga
     2281 tctggcgatg cgacgcgatt tccgcccggt agcctactgg aacccggcgt tgcagccgga
     2341 tgcgcagggt aaggccagaa ttcgcttcac gctgcctgat tcgaccactg cctatcgtgt
     2401 ctacgtggtg gcactcgatc gtggtgacgc ctttgcttcc gtcgatcgtc agatggtggt
     2461 caccaaggag ttctatgtcc agcccggtat gccgcggttc ctgaccgtgg gggacaaagc
     2521 ggtgtttccg ctctccgcct gcaataagac cgaagacgca ctcgaggctg atctgaaggt
     2581 cgaagggcgc aatgtgctgg tgaaactgtc gcagccgaag gcttcgatga agcccatgac
     2641 caacacgccg gtacccgtcg aagtcgaagc tgatacgcat gcgcctgaag cactgctgac
     2701 cttccgtggt accgcggggg cgttcaatga tggaatcgaa ctgcccctgc cgctgcagcc
     2761 tcgacgtctg gcgatcaatc gggcgcgcat cggacacttc accgataagg cggagatcga
     2821 tgccgagatt cccgcggccg tatcgctgat tccggaaaat gagcggcgcg gtgttctcac
     2881 ggcgattgtc gctctatcaa ccaccgagtg gaccaaagtc gcccccgggt tgcggtatct
     2941 gctgcagtac ccgtatggct gtatcgaaca gacgagttcc ggcatcattc cgctcgcggc
     3001 actgcgtcgc atggccgctc agaatctgat tcccggcgtg acgaaggaaa ccgtcgacaa
     3061 gttcctcaag gctggcgttc tccgggtgtt gcgcatgcag cgttcatccg gcgggttcgc
     3121 ttactggccc gaacagtccg aagtcacgtg gtggggaacg ctctacgcca cgttcgctct
     3181 gacggcagca cagcaggcca aactcgaaga tccagatgcg gccttcgagg ttcctgagag
     3241 tgctctcgaa aaagccctct cgtacatcgc acaacgggtg ctcaaggaaa aggacagttc
     3301 agattggcac tactacggcg tgtatgagct ggccgcggtc aatctggctc tgaacaacaa
     3361 gctgaccgct gctgatctcg accgactgct ggccgagttc gacaagcgct ccgaagaggg
     3421 caaagccttc ctgatctggg cggcggcggc atcgaagcac ctcactccgg cgcggatgaa
     3481 agatctgctc aagaagctca atcccaagct gggtccgtct tcgacgacct ggtacgatag
     3541 cggaacacgt gaacttgccg cggcgctggc ggcccagatg caggctgatc cttcgtcacg
     3601 gaaagcggat gactacgccg gcagtctgct caagtccgtc aatcgtgaag gatactgggg
     3661 ctcgaccgct cataccggtt ggtgtctcta tgcgctctcc cagtatctga ccaagaagat
     3721 gctcggcaag ggtgaggaag tgcccgtcga agtccgacag ccgggccgtg accccgtgaa
     3781 agttcgcgta ggtcagaaca gcagtgaggt cgagctcgat gccgaggctc tcctgaagaa
     3841 tggaaaagtc cagttaacca ccgccggaaa gaagctggta aactggacct tgatggtgac
     3901 gtatcctgat acggcgacca gcgctgaaga tctgaatcag ggattcctcg tggagaagaa
     3961 gatcaccaac ctgaatgggt cgaacgagtt ccgggtgggt gatatcgtca aggtgtcgat
     4021 cgagttcgcc gatactccct ctcaatcaca aaactggcgc aactaccgca actatgagta
     4081 tctggctctc gaagacccgt tgccggcagg attcatggcc atcaataccg ctctcaaaaa
     4141 tgatcgtcga ccggtgacca cgaacgagga tggcgaagag tccagttccg atgacagtga
     4201 aagcgaggaa gattgggcac cctgggaaga cggagcctat ctcttccgcc ccgatcactc
     4261 cgagatgcat cccgagaagg tggtggcttt ccgtgatcgt ctctggtcga accgtttccg
     4321 cttcacctac tttgcgcgtg ccatctgtga gggcgagttc atgcttcgcc ctacgcgcgt
     4381 ctcgttgatg tatgacccgg aaacctacgg gttgagtcag tctcagacag tgaaggtctt
     4441 gcccgccaaa tagcatcggc acgctcgaaa gaatcaggcc cggagatttc tccgggcctg
     4501 tttctttcga gcatcattcg gttggtcaac ctgaacagaa gtccaggtta caaggctcga
     4561 aaagatgctc gggatgcaat aatgaactgt cagttacttg gattccttgg cccactcttt
     4621 cttggcatac tcttcgaggc cgggatgatc ctggaggaac tgatcctttt ttcgctgtgt
     4681 ttcgtcgatt ctcttcttca gatctgctat ctcatcgtga agctgcagca attcggatcg
     4741 tttggcatgc aggcccttaa tctccgcatc gagcttggcg atctcttcct cgagctggcg
     4801 agacgagctg atctcacagc cagtcgttag aaggcaactc aagagaatgc cgaaaagaag
     4861 gtggtgtttc ttcatttctt ttctgcctcc cgttgcttct gatacttttc gtacagctcc
     4921 agctgtgcct caaggttgga aagctcttga cgtacctgat cgcgctcgtt gaacagatga
     4981 acatggcgtc gaacttcgcg atcagcattg tcgcgttcct cgagcagttt ttcgcgctgc
     5041 cttctgagtt tccccgaaaa gtccatacag ccgatgaaac ccggaagcag taacgcgccg
     5101 agcaaaacaa ccgcgaatcg tctgcgaaga ctattcatca tctggaatct cttgggttga
     5161 catggttttg gccgcatact tgcgcaactg tttcagcgcc acctcgaagt cccgcggcag
     5221 cggtgcgatc agttcaagtg cgtcatttcg tcggaagtgg tgaaagctca gtttccacga
     5281 atgcagtgac agacgaccga tgagcgggct ctccccttct ttgccgggtt tgtacgattt
     5341 cttgaacgct gaaagcagga tactgccagc acctccgtag tcggagtcga ccgcgagtgg
     5401 gaacccgata tgagccaggt gaacgcgaat ctgatgctgg cgaccggtac gcggcttggc
     5461 ttcgatgaga gtgagcccct tgaatcgctc gacgactcga tactcggtga ccgcgggttt
     5521 gccggttttg cgatcgatct tcatcaggcc tggttgatca gggtgttcgc cgatgggcgc
     5581 atcgatgacc ccctcgtcgt tgggcatctc gcctttgacc agagccaggt aggtcttctg
     5641 aatctcctgc cgggcgaacc gatcgctgag atcgacgttg gcttcgctgt tgcgcgcgaa
     5701 cacgaccaga ccgctggtgt cgcggtcgag acggttgacc ggatgcacct tcgcgaaacg
     5761 ctgtgacagc agatcatgca ggttgagcag atgcttgcga aaacgatcgg gaatcgagag
     5821 caggccggaa ggcttgttca cgaccacgaa gtcttcgtcg gtaaagacta tctcgagatc
     5881 tgtgggccga atgcccccat ccggattacg cagactgcgc gtaagacgtt tcatgggtgt
     5941 gactccttcc gggtcaggcg atagccttct gccgccgtca gcagagcctg aaacaggccg
     6001 tgaaaacgag gatcggttgg cagcgccctc tctgggtgga actgcacacc cacgaaaaat
     6061 gggtggttcg tggcttcgat ggcctcgatg attcggtcat ccgcccgcgc ggtcacccgc
     6121 agatgttcgc cgagtttctc gatcgcctgg tggtgaaccg aaggaaccag cagtcgccgg
     6181 gcttgcagtg cctgccacag cagactgtcg ctttcgatgt tcaccgcgtg cgtggccagc
     6241 ttcgcttcgg ccccggccgg gcgtcgatgc tgaaagcgac cgctgcgctg ggtctcgctg
     6301 ggaatgtcct ggatcatggt tccgccacgg tgcacgttca tcaactgtag acccttgcat
     6361 attcccagga cgggcatctg acgccgatca gccattttca gaacctcgag ttcgaaacgg
     6421 tccagtccgc gttcgacggt ttcgagcttc ggatgaggtt cttccccata cagtttcgga
     6481 tcgatgtcat cgccgcccgg aacgagaagc ccgtcgagca tcttcatctt gcgcatgatg
     6541 gtcgaagcct cgtcggtgac cagcagagcg atcgctcgac cgccgttttc gatgatggcg
     6601 cgcgaatacg acttcagagt gtcgcgtccc cgtgccatcg ccagtgactg agagtgattg
     6661 tagagaacgc cgatcttcag ttcggcatct tgagcgttga gccgttgaga cagggccaga
     6721 atgatgacga tgcacaggca cagcaggaag aggcgataac ggcaaagctg cgaatgtcgc
     6781 atgcgggatt ctcctcgaag gcagtttagg ctaaaatacc accagaagcc ttcttttcac
     6841 agctgcggag aaacaagggc catggttcag ccaccgaccg tcaccggaat caatcatctg
     6901 acctttgcgg ttcgcgacct cgaccggtcg ctggctttct acgtcgaggt tctgggcctg
     6961 tgtctcgttg cccgctggca gggcggtgcg tatctgcgag ccggcgcagt ctggatcgcg
     7021 ctgttcgtcg acgacgcggt gcgatcggct ccgcatccgg actacacgca tacggccttt
     7081 ggggtatcgt cggcagggtt cgccgagctt gcggctcggg tgctcgggtg cggggcagaa
     7141 gtctggcagc agaatcagag cgagggcgct tcactctatg ttctcgatcc tgatggacac
     7201 aggcttgaac tgcacgtggg cgacctggaa gaccgcgtac ggtcgttgcg cgcgaatcct
     7261 ccccctggtt tgcaggtgtt tgtatgatcg agactcgccg tgaacgctgg cgtcggctgg
     7321 ggtggcgttt ggcgaaactg gccctgctgg ctggtgttct cgcatggatg ctgtgggaat
     7381 tcgctctcgc tttcctcatc cctgactatt cggcgcgggt acgcgcggct gtcgccgacg
     7441 gcggcgtgct gctcgaccgt gatggccgca tgctgcgcct gtttccgaat gccgacgacg
     7501 agttcctgct gagttccccg cccgcgtcgt ttcctgaact gttgtgtcag gcggttctgg
     7561 ctgccgaaga ccggcgattt tacgagcatg cggggttcga cccctgggcg attgtccggg
     7621 cgttctggct caacctgcgg caccaacggg tgatctccgg agcgagtacc atcacccagc
     7681 aggtgatccg catggtgcgc ccggcttcgc gaaccattct gaccaagatc agcgagctgc
     7741 tgctggcggc gcgtctcgag cgcagtctga acaagaccga gattctcaca gcctatctca
     7801 acctggcgcc gatgggcgga aacgtgcgcg gagctcctct tgccgcccgg gtattctggg
     7861 gaaagtcgct cgagcagttg acctgcgcgg aagccgctgt tctggcggga attccacagg
     7921 ctcccagccg actcgatccg aaccgctgga aagggcatcg attgctgaaa aagcgtcgga
     7981 actggattct gcagagtatg cgggaactgg gtttcatcac ggtggaagag gaaaagcagg
     8041 ggtgtcaggc acccgtctgg gcgaatgccc gtcggttccc ctttctggca ccgcatttct
     8101 gcgattgggt tgtcgcgcag atcggtcgtc tacagggctg gatcaccacg acggtcgatc
     8161 tcgacgtgca gcagatggtg gaagagacgc tgcaggcgca cgaagcgcgc ttgagacgtt
     8221 caggagccgg gcaggcctcg gtactggtgg tcgacacccg ctccatgcag atgctggcca
     8281 tggtcggttc cctgcgtttc ggacctctgg cgggaggcta caacaacggc tgtgtgtcgc
     8341 cacgctcggg tggatcagtg ctcaaaccgt tcctgtatgc cctggctttc gagaatgggt
     8401 ttcacccctg ttcggtggtg ccggatacgc gacagcattt tcagaccccg cagggagatt
     8461 acctgccgct gaatgccgat cggcgagcct atggccccgt caccattcgt caagctctgg
     8521 gaaactcgct gaacatcagc gccgtgaaaa tgctcaacgt ggtcggggtg cgaacattgt
     8581 atcaactgct tgtcgatctc gagcttctga agcctgatcc ctccggaccg gaccggtacg
     8641 gtctgggact ggcgattggc aatcctgaaa ttcgcatgat cgacctgata cgggcgtatg
     8701 gggctttcgc gcatcgagga gctttgaaac caatcacgta tcttgcgagt tcgacggctc
     8761 cattgcgttc gccgctgttg acgcaggaat ctgcgtttct gatcctggac attctttctg
     8821 atccgacggc ccgcctgttg accttcggca acccccgcta cttttcgttc gacgggccgg
     8881 tggcgctgaa aaccggtacg agtaccgact atcgtgactg ctggctgatt gcggtgactc
     8941 cgcgttacat catcgggttg tgggccggca acttcgatgg tcggccgacc ttcgggcttt
     9001 ccggggcgtc tgcgtgcggc ccggtgttgc gcgatctgct gcagaacctg ccgggggctt
     9061 tgtctgagcc ctggtttcgt gtccccccgg gaatccgtga gatcccgatc tgcggcattt
     9121 ccggtcagac accgactccg ttttgtgacc gactgtctcg agaattggtc agtcgcgatt
     9181 ctgaagtgcc tcaaacttgt acgtttcata ccagtaacgc ggcgactcac gaattgccca
     9241 tggagtatgc ggactggctg cagcagcgac gcctcacgct gcaggacgat ccattcacgc
     9301 tcagccacgg tgtggcaacc ccggacccat tgcgcccgtc ctcactgatg cctgggacag
     9361 cccagggcgg agtgcgtgtg cgtgcggtcc agcccgacgt gttgtcctgg tggccgtttc
     9421 cgtttaacgc gatggtttcg cagtatcgca gcctgggaga tcccgggagt caaagggacg
     9481 tgagtttcgg ttggctggct tctgccccgt tgtggctttc tgcccgagct gtttctgaga
     9541 cagcggttgc ttctttcgcg tcccccgttc cggaaccttc gcgccgcatc accgtctccc
     9601 agggcctgga tcgttccccc ctcgcgcagg ttggtcgcat taccattctg cagcctcacg
     9661 atggcgatcg gtttgtgctc gttccagatc aggaaaatgt gacccgcttt cgcgcggttc
     9721 cggaagaacc cttgccggaa atcatctggt tgctgaatgg gcgcgaactt gcgcgaaccc
     9781 ctccgcccta cgagttcttc tggaccatgg agaaagggcg ccacgagatt gctgcgattg
     9841 gccctggaga gcagggcagc agcatcatga tcgaggtcga gtagcgctac agactcatca
     9901 acagatcggt cagtttgttc atgaccgcat cgcgtgactt ggccagagta aactgcacga
     9961 ccttcttcag cttctccagg tcgcctttca tgcgcttgat ctcgtcgcct tcgacctgct
    10021 tcagatcagc atcagagaag gtcagccact cgccgagtct cgccacggcc tcttcggcat
    10081 acttgccctg gggcttggct tcaagatacc gtcccagttt ttcccaggcg ttgacgacaa
    10141 ctgcgggcgg atacccttcg gtttcgccgg gcagatcctg gctggcggcg gcccaagcca
    10201 gttcgtcggc aaaggcgtgg tcgcgatacc gctggtagag atcccagaac aggtcgcggt
    10261 tcaccagaca ggctgcagcc ggctcgttcc agatgagctg ggacagtaac ccgcgcaccg
    10321 acggcaccgc ctcgagctct tcgacgttct tcacctgaca gaaatcgaga tacttctgca
    10381 gggcgcgtaa atgtccgagt tcaaaaaatg gtttatcatc tgttgaagta attgttggat
    10441 tcacacgatc aagaaactcg aacagttcga gccagtcgag cgcattctgc gaatcttgag
    10501 ccagacgttc ttcgaccaga cgccggtaaa gcgcttcgcg tctgctgtcg tcgaaatcaa
    10561 tggtgaaacc accaaaaacc catcctgagc tcccgctggg caacttcaac ttgtaccaat
    10621 gatcctgttt tttgccgatc gtctcgggct tgctgctttt ctccaactgc tcaagcacat
    10681 cgccgaaatg caacttcaga acctcggccg acttggtggt cggctcggct cgcacgcgca
    10741 ccgcggctcc ggtgatcagg cgttgagccg cgaaaagcgg aagatcagaa gcggcgaaca
    10801 gcagagcgct cacacaggcg gtgcccagaa agcggcggac ccaggtcatg gtaggcatgg
    10861 ttgttcctcc ccgtacaatg agtgaatctg cgtcaagttt accgtggttg ctcccgttgc
    10921 gccacgaaaa acaacgcccc cgcgagcggc gggggcgttg cgaacatcga cgttgcctgt
    10981 gtcaggcgtg ttcagccttg ttgttcagtt cgaccgggcg tgtccagccg aaggggttcg
    11041 agatttctcc gtattggatg ccgagcagca tgtcgaacag cttctgggtg atcggaccga
    11101 cccggcgatg cccgacgacg taatccttct cgcgatactt caggttgccg acaggcgaaa
    11161 tgctggcggc ggtgccgctg ccgaaaattt cggtgatcga acccgtcgta ataccgccga
    11221 cgacttcgtc gatcgacagt tctcgttcct cggtcttcag gcccagatgt ttccccagca
    11281 ccagcacgct gtcgcgggtg atccccgaga gaatcgagcc attgagcttg ggtgtgacca
    11341 gagtgtcgcc gttgaacacg aagaagatgt tcatcgcgcc gacttcctcg acgtagcggt
    11401 gatgcactcc atccagccac agcacctggg cgcacccctt ttcgcgggcg acgcgaccgg
    11461 ccagcaggct ggcggcatag ttgccaccg
//
LOCUS       DAORHD010000009        10582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_5538_length_10582_cov_4.303695, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000009 DAORHD010000000
VERSION     DAORHD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10582
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5538_length_10582_cov_4.303695"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="PKO06_00485"
     CDS             <1..537
                     /locus_tag="PKO06_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68141.1"
                     /translation="SWIVRKTAAEAIWEQGYGLSDELLSASSAPNTDSRFWSVKILGR
                     MNEPTAFPRLIERLQDPDPSVRAAACQALGSLGNRQALAPLMTLLADPSEEVRTSAVL
                     AMSQIGEKDDKQAHRPSMPSHLRPENQQTCGQCGKKVNRDFTYCPFCLASLGTSCRKC
                     GRSLQADWKGCPDCGQRQ"
     gene            complement(575..3913)
                     /locus_tag="PKO06_00490"
     CDS             complement(575..3913)
                     /locus_tag="PKO06_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV68142.1"
                     /translation="MRTPASLRLALAAAFLLSALVWWCQPQSACTAPSLPPHLPSPPP
                     LGLSAEERAWLAQNRLVRIGVTPDFPPYEFLDASGTLRGLVGDTVALVAHQLQLELVI
                     EPDLPSWQHVMQALQSGKIDMVASAKITEERKQFMHFSPPLIELQSVVFTLDQARYTD
                     FSSLAGKRLASLKGWADTGWVASNCPQIRLVPCSRVEDLFGQVLLQGADAGLLDIGSL
                     GHYAHEHHLSQLSIAFTTPLTYSLAIGVRRDLDILARLVDRALKNIPPSTRTLLEHQW
                     LKSPQPEIYQQVALILVALAVVLIGFVFWNIQLRATVNRRTAELERSVAASNRQTTAL
                     QESERKFRAVFNQTFEFMGMVSPDGILLAANRPALEFIGMDEACVVGRLFWETPWWQD
                     LPQSQQLVRDAVGSAAKGEFARCELPARSRTGELSVLDFSMTAVRDDAHQILFLVPEG
                     RDITDLKAAQAAAQEGEQRFRDLFELSPDPCLLIDQDQFIECNRAALRLLGFDQKNEL
                     VQKHPADISPPTQPDGRNSVDKAAEMTAIARKRGMYRFEWVHRRRDGVELFVEVTLAT
                     IAFQGRSMLYCQWRDLSERRQTQAALRESRALVSAALETLPFDFWMCDSEFRLTLQNP
                     GSIQHWGQQLGHRLDEFLSPSTIGVLRADIQRALKGEFLQVERAFPRHNSTGWYRMIL
                     APIRDNNSILGALGILIDITDRKQAEDEKQLLEQQIRQAQKIESIGRLAGGIAHDFNN
                     YLTAILGNAELGRTELPPDHPSHATFQEIVETAQRASSLTRQLLTFSRKQPNQTQVLD
                     LNRLLSEIRKMIERLIGEQIQVVFQTPATPSCIRADPGQMEQVLVNLAINARDAMPNG
                     GQLSITLSHVDNPSHRLGRPPEIPNGQFICLSIRDTGIGMSEDIQQRVFEPFFTTKAA
                     GKGTGLGLATVFGIIRQSGGWISLESSPGAGTTFFVYLPLVDASPADLSVGGPTASVP
                     AGTPTRETIMLVEDEDSLRKLIVRFLTKQGYVVPAYPSGESALEALADPQAKPPALLI
                     TDVILPGITGRVLAEKLRQRFPELPVLFTSGYSDEVIETHGVFKDGLEFLPKPYTPYV
                     LAQHVRKILDHASRLSEKKTGDSR"
     gene            4085..4654
                     /locus_tag="PKO06_00495"
     CDS             4085..4654
                     /locus_tag="PKO06_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HNV68143.1"
                     /translation="MKPEAFDALFHDLLNLKKLRRTGWALRGIRDGESLADHAFGVSI
                     LTLILVEFHPQLNREKAVRLALLHELGECRVGDIPFPALSYLKGKSEAERAAVSDLLE
                     PLGERKPSLLAEYLEFEDNQTPEARFVKAVDKLEMLVTAAEYERTGYRSLTDFWENAV
                     TFECLQEFPLLSELANRLRQLRTERLGSR"
     gene            complement(4679..5407)
                     /locus_tag="PKO06_00500"
     CDS             complement(4679..5407)
                     /locus_tag="PKO06_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNV68144.1"
                     /translation="MLNILRSRTFILIIVFLSITLSFYPFERTSFSQSKTAPEDQGKV
                     VLALPEPLQTGLHSLEETLARRRTVRSFASRPLTAAQASQLLWAAQGVTSPEGYRTAP
                     SGGGLYPLEIRMVAGNIEGIATGTWHYQPASHQLSLEHPADIREALIDAAYRQTWMGQ
                     GAAIFVISGVSARSSAKYGTRGVRFTYMEAGHAAQNLLLQAIGLGLEAGTVGGFDDAK
                     VRAALHLPAEEEPLYLLVVGSPAP"
     gene            complement(5600..6640)
                     /locus_tag="PKO06_00505"
     CDS             complement(5600..6640)
                     /locus_tag="PKO06_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNV68145.1"
                     /translation="MPGGFLLKRLAVFLFLALVVWITLTALLTDVGRVTQLLSRLSLR
                     WLLAIVGAVLFNYALRFLKWSFFLRLLEIRLSLTDSLWIFFSAFTMILSPGKVGELVK
                     SVLIRSRYGIPAARTAPIVMAERLTDLLGLTALAAIGYSRYSYGGHALELIGGIIAAG
                     TLLITRPRFWILLDRFFLSRLPRFSRLRGSLLVLQESTANLLSLRSLAWTVPLSALSW
                     AGEGFALYFIFQALGVDRPDLLGISLFAHAFGSILGAVSFLPGGLGVTETTMGGFFVL
                     VGFSSELSVSATLLIRALTLWFGVLLGTIVYLFGRRPGDVLLMPGTPVTPSSAATSEA
                     TSASRVAGDTRD"
     gene            6751..7707
                     /locus_tag="PKO06_00510"
     CDS             6751..7707
                     /locus_tag="PKO06_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68146.1"
                     /translation="MRRSVLAFLGGILWAMSGAIDAAPLVLFTGEATETRDLYQIDLA
                     NMSIQPVLKTGDNEEMAAVSPDGKTLCFVSDRQGAMSLYRLSFPVDQATGTDISGGMG
                     AYSFPAWSPDGKTIAVSYAPDPEARLFKRKLVLLDPETRTQKVLLDSVTWTKADGVEV
                     VLDRPLWIDDKTLVFVAIEYADPADAPRIVSSTLYRLTLPDGKPERLVGGESYFDASG
                     SSKGFSASLPTRHRAGVAFSAIEGRFRRTPMTTTLDGLKKQVIPLKDANYFGPYLEIG
                     NACIYGIQDEEGRLKLMYQADEKQKPVPIPFAGDSYEPVLMP"
     gene            7712..9445
                     /locus_tag="PKO06_00515"
     CDS             7712..9445
                     /locus_tag="PKO06_00515"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNV68147.1"
                     /translation="MDVWTLRRHVRELNLWLREHPVVLRIVECIPSGWGLVLRRADGT
                     TGCLELHLDGEKQGFLWNPSWVETPAESALGRAMHRRLADARAGEAQVLGYDRVVHVP
                     FQRRDRLFGSIERSALILECTGRVANLLLVDEHREIIEQARLTANNRPKTEYQPISSD
                     LPELNPLNLDPDAGRKILALPSSEWRHDLPWLSPVLCRELEFRLGHGVVDPVRVLIDL
                     IGELEGNRPVSVVWRDAKVRAISTAELRHLADSPRQSFATVNEALIWIDRELIEARRD
                     RELRGRALGHFRKRLLRLEQAIAEEQTRLAGFDQAETWRHQGDLLLANAHAIPARATE
                     ARVLDWDTSAEIVIPLDPEATVAYCARRLFQRYKKALRGQEEGRKRLQTLEREAAWVR
                     EQIWFCEHAAGAAELHDLLPSASKRRRPTGSGDGRKDARERQESKRISPALELDGCRF
                     YVGRNGRQNDLITFRIGRRGDLWCHANDVPGAHVVARRADGPVTDEDTHRGAVLAAYF
                     SFARQGGKVRVDVTDIAHVKRIPGGDPGRVSYTHQRTLTVDPLEAESWLTVSPPTVGE
                     KKTPAAPGDGG"
     gene            complement(9456..>10582)
                     /locus_tag="PKO06_00520"
     CDS             complement(9456..>10582)
                     /locus_tag="PKO06_00520"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNV68148.1"
                     /translation="LFFVALPLLFWNEGRAVTTALSLEEGLGAVVTVAADKVDPANEN
                     KLVHMSASTKVEGTLSDDEFGVSAPAVRLRRSVEMFQWQEEKKSEEKKKLGGGTETVT
                     TYNYKKVWSSVAIDSGSFKKSSEHQNPGSIPYSSKSFEASQVQLGAFTLSPSLVGKIS
                     NWTPVDPASKPATGEAVSGTTASGTTTAPMFPGGLKAVNGKLYSASADPSNPKIGDIK
                     ISFEAVLPSTISIIAQQTGQTFRPYQTKAGDSLEMLDNGNRSAQEMFASAQSANTMMT
                     WGLRIFGFLLMYIGISMIFNPLVVLADVIPFFGNLMGAGVGMLSFFLAAPLSLTMIAI
                     GWIAYRPVLGIALLVFAVAIFVGGFMMMRGKKPAAAPLQA"
BASE COUNT         2272 a         2919 c         3356 g         2035 t
ORIGIN      
        1 tcgtggatcg tccgaaaaac ggccgccgaa gccatctggg aacagggata cggcttgtct
       61 gacgaactgt tgtcagccag ctcggcaccg aataccgatt cgcgcttctg gtcggtgaag
      121 attctcgggc gcatgaacga accgacggca ttcccgcgtc tgatcgaacg attgcaggat
      181 cctgatccct cggttcgtgc cgcggcatgc caggcgctcg ggtcactggg caatcgacag
      241 gctttggccc cattgatgac cctgttggcc gacccatctg aggaagtgcg cacgtcggcc
      301 gtgctggcga tgagtcagat cggcgagaaa gacgacaaac aggcgcatcg tccctcgatg
      361 ccatcgcact tgcggcccga aaaccagcag acatgcggac aatgtggcaa gaaggtgaat
      421 cgcgatttta cctactgccc cttctgcctg gcgtcgctgg gtacgagttg ccgaaagtgt
      481 ggacgctctt tgcaagctga ttggaaaggc tgcccggact gcggacagcg acaatagaac
      541 gacgacctct gcagatcagg ccggcgattc aggctcagcg tgaatcgccg gttttctttt
      601 cggaaaggcg cgaggcgtga tcgagaattt tgcgcacgtg ttgcgccagc acgtaaggcg
      661 tatacggttt tggcaggaac tccaggccgt ctttgaagac gccatgggtc tcgattacct
      721 catcagaata cccggacgtg aacagaaccg ggagttctgg aaaccgctgg cgaagcttct
      781 cggcgagaac acgcccagtg ataccgggaa ggatgacatc ggtgatcagc agggccggcg
      841 gcttcgcctg cgggtcggca agtgcttcga gagcgctttc tccgctcggg taggcaggaa
      901 cgacgtatcc ctgtttggtg agaaaacgaa cgatcagctt gcggagtgag tcttcatcct
      961 cgaccagcat gatggtttct cgcgtcggcg ttcctgcagg cacggaagcg gtggggccac
     1021 cgacggagag gtcggcagga gaagcgtcga cgagcggcag atagacgaag aaggtcgttc
     1081 cggcacccgg agagctttcg agagagatcc agcctccgct ctgacgaatg atgccgaaga
     1141 cggttgccaa cccgagaccg gtgcctttgc ccgccgcttt ggtcgtgaag aaaggctcga
     1201 aaacgcgctg ttgaatatcc tccgacatgc cgattcccgt atcgcgaatg ctgaggcaga
     1261 taaactgacc gttgggaatc tcgggcggtc gaccaagacg gtgagacggg ttgtcgacat
     1321 gactcagggt gatcgacagc tgtcccccgt tgggcatggc gtcacgcgca ttgatggcga
     1381 gattgaccag cacctgctcc atctggcccg ggtcagcgcg gatacaggag ggagtcgccg
     1441 gtgtctggaa cacgacctgt atctgctcgc cgatcaggcg ttcgatcatc ttgcgaatct
     1501 cgctgagcag gcgattcagg tcgagaacct gcgtctggtt cggctgtttg cgcgaaaagg
     1561 tcagcagctg gcgtgtcaga gatgaggcac gctgtgccgt ttccacgatt tcctggaatg
     1621 tcgcgtgact cggatggtcg ggtggtagtt cggtgcgtcc gagttcggcg ttgccgagaa
     1681 tcgccgtgag atagttgttg aagtcatgag caatgccgcc ggcaaggcgg ccgatcgact
     1741 cgatcttctg cgcctgcctg atctgctgtt ccaggagttg tttctcgtct tcggcctgct
     1801 tgcgatcggt gatatcgatg agaatgccga gtgcaccaag aatagagttg ttgtctcgaa
     1861 ttggagccag gatcatgcga taccaaccag tactgttgtg gcggggaaaa gcacgctcga
     1921 cctggaggaa ctcacccttg agggcgcgct gaatatcggc tcgaagtacc cctatggtcg
     1981 aggggctcag aaactcgtcg agtcgatggc ccagctgctg gccccaatgc tgaatggagc
     2041 ccggattctg caacgtcagc cggaactcgc tgtcgcacat ccagaaatcg aagggcaggg
     2101 tttcgagggc ggccgaaacc agagcgcgac tctcgcgaag tgcggcctgc gtctgccggc
     2161 gttcagacag gtcgcgccat tgacaataca gcatcgatcg accctgaaag gcaatggttg
     2221 ccagggtcac ttcgacgaag agttcgacgc catctcgacg tcggtggacc cactcgaaac
     2281 ggtacatgcc gcgcttgcgg gcaatggctg tcatttcggc cgccttgtcc accgagttgc
     2341 gaccatctgg ttgggtcggc ggcgaaatgt ccgcagggtg cttttgtaca agttcatttt
     2401 tctggtcgaa gccaagtaag cgcagcgcgg cacggttaca ttcgataaac tggtcctgat
     2461 cgatcagcag acaggggtcg ggagacagtt cgaacaggtc gcgaaaccgc tgctcgcctt
     2521 cctgagcggc tgcctgtgcc gctttcaaat cggtgatgtc tcgcccctca gggaccagaa
     2581 acagaatctg atgggcatca tcccgaaccg ccgtcatgga aaaatcgaga acactgagtt
     2641 cgccggttcg gctgcgagcc ggcagttcac accgcgcgaa ctcccctttg gccgcgctgc
     2701 cgaccgcgtc ccgcaccagt tgttgtgatt gcggcagatc ctgccaccag ggtgtttccc
     2761 agaacagacg accgaccaca cacgcttcat ccatgccgat gaactcgaga gctggccgat
     2821 tggcggcgag aagaataccg tccggactaa ccattcccat gaattcaaag gtctggttaa
     2881 agaccgcgcg aaacttgcgc tcgctttcct gcagggcagt ggtctggcga ttggatgcgg
     2941 caacactgcg ctcgagttcg gcggtacggc gattgaccgt ggctcgcagt tgaatgttcc
     3001 agaacacgaa accgatcagc acgacggcca gggcaaccag tatcaacgcg acttgttgat
     3061 agatctcggg ttgcggtgat ttgagccact gatgttccag aagtgtgcgg gtcgagggcg
     3121 gaatgttctt cagggctcgg tcgacgaggc gcgcgagtat gtcgagatcg cggcgaacgc
     3181 cgatggcgag agaataagtc aggggtgtcg tgaaggcgat cgacagttgt gacagatgat
     3241 gctcgtgggc gtagtggccg aggctgccga tgtcgagcaa cccggcatca gcaccctgca
     3301 gcagcacctg gccgaagaga tcctcgacgc gactgcacgg gaccagacgg atctgtggac
     3361 agttcgatgc gacccacccg gtatcagccc accctttcag tgatgccaga cgcttccctg
     3421 ccagactcga aaaatcggtg taacgcgcct ggtcgagggt aaagacgacg ctctgcaact
     3481 cgatgagcgg cggcgaaaaa tgcatgaact gtttgcgttc ttcggtgatc ttcgcgctgg
     3541 ccaccatatc gattttcccg ctctgtaagg cctgcatcac atgctgccac gagggaagat
     3601 cgggctcgat gaccagctcg agttgcagtt gatgcgccac cagagccacc gtgtcaccga
     3661 ccaggccgcg tagagtgccg ctggcatcca gaaactcata gggtggaaag tcaggggtga
     3721 cgccgatacg caccagacgg ttctgcgcga gccaggcacg ctcttcagcg gaaagaccaa
     3781 gcgggggcgg cgagggaagg tgcggcggca gactcggggc tgtgcacgca gactgcggct
     3841 gacaccacca gacgagtgcg gataggagaa aagccgctgc cagagcgagt cgaagagagg
     3901 caggcgttct cacgctgtct gttggatagg ttcttgatgt gccgatcata ctctcactgt
     3961 tgttctattc taccgtactc ggcggttccg tatacagaga accttgactt gggtcgggta
     4021 ttcgtctacc tttcggcgcg atacgactca ggcgagcgag cccgaccacg ataaaggacc
     4081 acccatgaaa ccagaagcct tcgatgctct gttccacgat ctgctgaacc tcaagaagct
     4141 gcggcgtact ggttgggcgc tgcggggaat cagggatggc gagtctctgg cagaccatgc
     4201 tttcggggtg tcgattctga cgctgattct cgtggagttt catccgcagc tgaatcggga
     4261 aaaagccgtg agactggctt tgttgcatga gcttggcgag tgtcgcgtgg gtgatatccc
     4321 gtttcctgcc ttgtcttacc tgaaaggaaa gtcggaagcg gagcgtgcgg cggtgagtga
     4381 tctgctcgaa ccgctgggcg agcgaaagcc ctcgttgctg gccgagtatc tggagttcga
     4441 ggacaaccag acccctgagg cacgcttcgt gaaagcggtt gacaagctcg agatgctggt
     4501 gacggccgca gagtatgagc gaaccgggta tcgttctctt accgatttct gggaaaacgc
     4561 agtgaccttc gagtgtctgc aggagtttcc gctgctgagt gagctggcga accgactgcg
     4621 gcagttgcgt acggagcgct tgggaagccg ctgaggcgaa gcagaagtga cgtcaggctc
     4681 agggagcggg cgagccgacg accagcagat acaacggctc ttcttccgcc ggcaaatgca
     4741 gagcggctcg gactttggca tcatcgaacc cgccgacggt accggcttcc agtccgagtc
     4801 cgatggcttg gagcagtaga ttctgagccg catgcccggc ttccatatac gtgaagcgaa
     4861 cgccgcgcgt gccatacttg gctgaggaac gggccgacac acctgagatg acgaagatgg
     4921 ccgcgccttg tcccatccag gtttggcgat aagcggcgtc aatcaaggct tcgcgtatat
     4981 ccgcgggatg ttccaggctc aattgatgcg atgccggctg ataatgccag gttcccgtgg
     5041 cgattccttc aatattgccg gccaccatgc gaatctcaag aggatacaga ccaccgcccg
     5101 agggggcggt acgataccct tcaggactgg tgacgccctg ggcggcccac aacagttgcg
     5161 acgcttgcgc ggcagtgagg ggacgcgagg caaacgaacg cacggtacgg cgacgcgcca
     5221 gcgtttcctc gagggagtgt aaccctgttt gcagaggttc cggaagtgcg agaacgacct
     5281 tcccttggtc ttcaggtgca gtttttgact gtgaaaaact cgtacgttcg aacggataga
     5341 agctcagggt gatgctgaga aagacgatga tgagaataaa cgtacgtgag cgaagaatgt
     5401 tcaacatgtc cagtttgtga atgaatcggt acctattccg aagccggtgt agggattagt
     5461 gctctcaacc ggcaacactg taccgcagtt cgccgaaaaa gaaaagggtg agaaagaaat
     5521 agttgtgact cgagcgaagg aggcaggatc aggcgattgg acaggtgcga gtcaaatgct
     5581 ggtgcggctc gtgccagtgt cagtctctgg tgtcgcccgc aactcgagac gcggatgtcg
     5641 cttcagaagt tgccgcagaa gaaggagtga caggagttcc aggcatgagg agaacatctc
     5701 ccggacggcg cccgaacaga tagacgatag tacccagcaa gaccccgaac cacagggtca
     5761 gggcacgtat cagcaacgtg gccgacacgg agagttcact cgagaagccg accagcacga
     5821 agaaaccacc catggttgtc tcagtgacgc caagcccgcc cgggagaaag gagaccgcgc
     5881 cgagaattga tccgaaggcg tgggcgaaga gagaaatacc gagaagatca ggacgatcga
     5941 ccccgagagc ctgaaaaatg aagtagaggg caaacccttc accggcccac gagagcgcgc
     6001 tgagtgggac tgtccaggcc agcgaacgca gggagagcag gttggcggtg ctttcctgca
     6061 acacgagaag tgagcctcgc aggcgtgaaa accggggcaa acgactcaga aagaaccgat
     6121 ccagcagaat ccagaagcgt ggccgggtga tcaacagcgt tcctgccgcg atgataccgc
     6181 cgatcagttc gagagcgtgc ccgccgtaag agtaacgact gtagccgatg gcggccaggg
     6241 cggttaaccc aagcagatcc gtcagccgtt cagccatgac gatcggtgcg gtacgcgcgg
     6301 cgggaattcc gtatcggctg cgaatcagga ccgatttcac cagttcgccc actttgccgg
     6361 ggctgaggat catggtgaag gcggagaaaa agatccagag gctgtcagtg agtgaaagtc
     6421 ggatctcgag cagtctgaga aagaaactcc acttcagaaa ccgcagcgcg taattgaaca
     6481 gaacggcccc gacgatcgcc agcagccagc gcagggaaag tcgggaaagc agctgcgtga
     6541 cccggccgac gtcggtcagc aacgcggtga gagtgatcca gaccacgagg gccagaaaga
     6601 ggaagaccgc gagacgtttg agcagaaaac caccgggcat gtgcggtact ctgccccaaa
     6661 ccaggacgaa atgcaaccgc gtggtgcgtg agggcgaacc gtgctaccat ggtgcatctg
     6721 cctgatgagg gctggaaggg agggaagaag atgcgacgat ccgtgctggc attcctgggg
     6781 ggcatactgt gggcgatgag cggggcaatc gacgcggctc cgttggtgct gtttaccggt
     6841 gaagccaccg aaacccgcga tttgtaccag atagatctgg caaacatgtc gatacagcca
     6901 gttctcaaga caggtgataa tgaagaaatg gcggccgttt ctcccgacgg aaagactctc
     6961 tgctttgtgt ctgatcggca gggggccatg tcgttgtatc gcctctcatt cccggtcgat
     7021 caggctacgg gtaccgatat ttcaggcggg atgggcgctt actcgtttcc ggcgtggtca
     7081 cccgacggca agacgatcgc cgtgtcgtat gcgcctgacc ccgaggcgcg cctgttcaaa
     7141 cgcaaactgg tactgctcga ccctgagacg cgcacccaga aagttctgct cgacagcgtc
     7201 acctggacga aggcggacgg agtcgaggtc gttctcgacc gcccactctg gatcgacgac
     7261 aagactctcg tgttcgtcgc catcgagtat gccgatcccg cggacgcacc gcgcatcgtc
     7321 agctcgaccc tctaccgcct gacgttgcct gatggcaaac ccgagcgact ggtaggtggc
     7381 gaatcctact tcgatgcgtc cgggtcgtcg aaaggatttt ccgcttcgct gccaacgcgt
     7441 catcgcgcag gagtcgcctt ctcagccatt gaaggccgct tcagacgcac gccgatgaca
     7501 accactctcg atggtttgaa gaaacaggtt attccgttga aagacgcgaa ctatttcgga
     7561 ccgtatctgg aaatcgggaa tgcgtgcatc tacgggattc aggatgaaga agggcggctc
     7621 aagctcatgt atcaggctga tgaaaagcag aaacctgtgc cgattccctt tgccggtgac
     7681 tcgtacgaac cggttctgat gccctgatac catggatgtc tggacgctgc ggcgtcatgt
     7741 ccgggagctc aacctctggt tgagagaaca cccggtggtg ctgcgcatcg tcgaatgcat
     7801 tccgagcggc tggggcttag tgctgcggcg agccgacggc acgaccggct gtctcgaact
     7861 gcatctcgac ggcgagaagc agggcttcct ctggaaccct tcctgggtcg agactccggc
     7921 tgaatcagct ctcgggcgag cgatgcatcg caggcttgcg gatgcccgag ctggagaagc
     7981 acaggtgctc ggatacgatc gggtggtgca tgtccccttt cagcgtcgag accgtctgtt
     8041 cggttcgatc gagcgcagtg cgctgattct cgagtgcact ggtcgagtcg ccaacctgct
     8101 gctggtcgac gaacaccgtg aaatcatcga acaggcacga ttgaccgcga acaatcgacc
     8161 gaaaactgaa tatcagccga tctcaagtga tcttccagag ctgaatccgc tgaatctcga
     8221 cccggacgcc gggcgaaaaa ttcttgccct gccatcgagc gagtggcggc acgatcttcc
     8281 ctggctgtct ccggtgttgt gccgcgaact ggagtttcgc ctgggacacg gcgtggttga
     8341 tccggtgcgg gtattgatcg atctgatcgg cgaactcgaa gggaaccgcc cggtcagtgt
     8401 cgtgtggcgt gacgccaagg tgcgcgccat ttcgaccgcg gaactgcgtc acctggccga
     8461 ttccccgcga caatcgtttg cgacggtcaa cgaagctctg atctggatcg accgtgaact
     8521 gattgaggcc cgtcgtgatc gggaacttcg cggtcgagcg ctgggacatt tccgcaagcg
     8581 tctgctgcga ctggaacagg caatcgcgga agagcaaacc cggttggccg gtttcgatca
     8641 ggcagagacc tggcggcatc agggtgatct actgctggcc aacgcgcatg cgattcctgc
     8701 gagggcgacc gaagcccggg tgctcgactg ggacaccagc gccgagatcg tcattccgct
     8761 cgaccccgag gcgaccgtgg catactgtgc ccggcgcctg tttcaacgct ataagaaggc
     8821 gttgcgcgga caggaagaag ggcgaaagcg gctgcagact ctggaacgag aagccgcctg
     8881 ggtgcgcgag cagatctggt tctgcgaaca tgcagccgga gcggcggaac tgcatgatct
     8941 gctgccgtcg gcatcgaagc gtcgtcgacc gaccgggtcg ggtgatggtc gaaaggacgc
     9001 gcgcgaaaga caggaatcca agcgcatatc acctgctctc gaactggacg gttgtcgctt
     9061 ctatgtcggc cgcaatggtc ggcagaatga tctgatcacc tttcgtatcg gacgccgcgg
     9121 tgatctctgg tgtcacgcca acgatgttcc cggcgcgcac gtggtcgcgc gcagagccga
     9181 tggtccggtg acggacgaag acacccatcg cggcgcggtg ctggccgcct acttcagttt
     9241 tgcccgtcag ggtggaaaag tccgggttga tgtgaccgat attgcccatg taaagcgcat
     9301 tcccggtggt gatcccggtc gggtgtcgta tactcaccag cggaccctga ccgttgatcc
     9361 gctcgaagcc gagtcgtggc tgaccgtgag cccgccaacg gtcggggaaa agaaaacccc
     9421 cgccgcacct ggcgacgggg gttgagaaaa cgagatcagg cttgcagagg agcggcagcg
     9481 ggctttttac cgcgcatcat catgaaaccg ccgacaaaga tggcgacggc gaagaccagc
     9541 agggcaatac cgagcaccgg acgataggcg atccatccga tcgcgatcat ggtcagcgag
     9601 agcggcgcgg ccaggaagaa cgagagcatg ccaacgccgg cgcccatgag gttgccgaag
     9661 aacggaatga catcagccag cacgacgagc gggttgaaga tcatcgaaat gccgatgtac
     9721 atcagcagga acccgaagat gcgcagaccc caggtcatca tggtgtttgc cgactgagcg
     9781 ctggcgaaca tttcctgcgc gctgcggtta ccgttgtcaa gcatctcgag agaatcgccc
     9841 gctttggtct gatacggtcg gaaggtctgg cccgtctgct gagcgatgat gctgatcgtg
     9901 ctcggaagaa ccgcttcaaa gctgatcttg atgtcgccga tcttggggtt cgacggatcg
     9961 gcactggcgc tgtagagctt gccattgacc gctttcaggc cgccggggaa catcggagca
    10021 gtggtcgtgc cggaagcggt cgtgccggaa acggcttcgc ccgtggcggg cttgctggcc
    10081 ggatcgacgg gagtccagtt gctgattttg ccgaccaggc ttggcgacag ggtgaaggcg
    10141 cccagttgaa cctgcgacgc ttcgaacgat ttgctgctgt agggaatcga tccggggttc
    10201 tgatgctcgg aggacttctt gaagctgccg gaatcgatgg cgactgagga ccagactttc
    10261 ttgtagttgt aggtggtcac ggtttccgtc ccgccaccga gcttcttctt ttcctctgac
    10321 ttcttttctt cctgccactg gaacatctcg acgctgcggc ggagccgtac ggccggggct
    10381 gaaacaccga actcgtcgtc cgagagcgtt ccctcgacct tggtgctggc ggacatgtgc
    10441 accagtttgt tctcgttcgc cgggtcaacc ttgtcggcgg cgaccgtgac gaccgcgccc
    10501 agaccctctt ccagagacag ggctgtcgtg accgcgcgcc cttcgttcca gaacaagagg
    10561 ggaagggcaa cgaaaaacag ga
//
LOCUS       DAORHD010000010        10291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_5786_length_10291_cov_3.396737, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000010 DAORHD010000000
VERSION     DAORHD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_10291_cov_3.396737"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /gene="truB"
                     /locus_tag="PKO06_00525"
     CDS             <1..823
                     /gene="truB"
                     /locus_tag="PKO06_00525"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV68149.1"
                     /translation="VLVLFINRGLKLLQYIPAQALDKTYLARVTLGRTTDTYDACGET
                     TAVHPHPINVTKEQILETLRGFVGEYLQKPPSFSAVKVKGKRAYDLARQGVALDLPER
                     PVHVSSIRLIRDYESGGSRHLVLRIHCSRGTYIRSLAHDLGHTLGCGAHLSYLLRERV
                     GVWSIHQSIAPRVIERRESVLALSGFMPLDRILPYPRVVINHEAEARIRHGNPLDPRD
                     IRSISAEHVSTPEEADEQSLVQVFSENGEFLALYKPDRTRVERGQGRLQPVRILV"
     gene            825..1787
                     /gene="ribF"
                     /locus_tag="PKO06_00530"
     CDS             825..1787
                     /gene="ribF"
                     /locus_tag="PKO06_00530"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HNV68150.1"
                     /translation="MKLVNFLGGHIPFGPCVLGLGTFDGFHLGHRELVRRMVSRAREL
                     QLPAVLFTFDLPPKKILDPQNFPGALTLPEEKFQLLLESGVDWVVFRTFDKQFAVTYS
                     QEFVRRILAEKLQAREVFVGFNFGFGFNREGTADSLATDLHELGIGCQILPRVSIDGV
                     TISSTFIREQIKSGNLDQATKWLGRPVTLTGTVIHGEKRGRQMGFPTANLSLEHPVKV
                     LPPHGVYDTIADTPFGSYQAMTNIGVRPTFARHTPLLEAHLLGFNGDLYSQTIRVRFY
                     RRLREERCFPSMESLIAQMKCDLEEVRTSSMTRRGHPRDMGQSS"
     gene            complement(1832..4660)
                     /gene="polA"
                     /locus_tag="PKO06_00535"
     CDS             complement(1832..4660)
                     /gene="polA"
                     /locus_tag="PKO06_00535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNV68151.1"
                     /translation="MKELDMSGCLLVDGNNLLYRAFYALEKQRLTNRSGEPTGALFGF
                     LRILLKVLRDERPECVGIAFDMARKTFRHQMYAEYKSTRRPTPSELLKQIPHAWDLCR
                     ALGFRLLMSEEYEADDLIGSAAESCKGNLPVRIVTGDRDLLQLIDDQVVVLLCRHNLS
                     ETTMMNREVFVQEYKFDPIRIVDYKALMGDASDNIPGVTGIGEKKATTLIQQHGSLDG
                     VYAALEQIANPRMKQQLIEGRESAYFSYRLATIKRDAPWGTGPEDLAWPGLEAALPAL
                     VPLLRRWNFLSMVQELERQYPDSPAWKPVQEETRPGGQPHEPSLPGLDEPSGEVGAPT
                     VMVEPVLQPVPGERLLVRTDEEFEQVLSQLGETVAIDVETDHLSPRQGRIVGFSLSCG
                     PERSWYVPVRHRYFGFQAEGQPDAALVFPRLAEALQTRRLLGHNLKFDLAFLHREGVT
                     GTGTPEDTMLAAYVLNPTRSNALKELARTELGLDAVTFEQVVGKGNFADVPLDKAAEY
                     AGQDVILPWLLREKFAAGFSARPELAKLYEQLERPLMPLLLEMETTGIGLNRSYLGEL
                     QRDLQSRLNQLEQRIHEYAGGPFNVNSNKQLQEVLFQKLALPSPKRTRTGLSTDSEVL
                     EHLADAHPIAQLLLEYRELAKLKNTYADTLADLVDQRTGLIHTHFNQTITATGRLSSS
                     NPNLQNIPIKSEWGRKVRRAFVPPVAGQTLLSIDYSQIELRLLAHFSKDPSLVEAFRH
                     GVDVHAVTAGRLFGKFPEAVTDEERKVGKTVNFGIIYGISAHGLARQLQIPRNAAQTY
                     IDQFFAGYPGVTAFFERNQQEAAEKMEVSTLWGRVRPLPELADRNRNQRAFGERVARN
                     TPLQGTAADLVKKAMLDAAKALCDGGFRTRLILQIHDELVFSAPPEELPAVSDLMTTT
                     MERVAALDVPLVCDAATGPNLADLAPVA"
     gene            4766..6937
                     /locus_tag="PKO06_00540"
     CDS             4766..6937
                     /locus_tag="PKO06_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HNV68152.1"
                     /translation="MLSIGRDILGNIKEAARLEWLVTNGIGGFASSSVLGMNTRRYHG
                     ILVAARRPPTERLAIVSRVEESLCVDGHEVFLSTNRYQKALYPAGYLHLERFDRDPLP
                     TWTFQYEDILVVKTLFMVHGENTTVITYKVFSNGRDVELRLRPHLLFRDFHGNTFENP
                     GFEDNTTVVDTFFSLQPFANAPALYMSWDRGTFVREAFWYRDNHLIEEENRGLNALED
                     DYSNGYLRVPKCQGTASICFSLTPPVKHANALELRKREELRLESIAQSLQSEDQFLRR
                     LLLAADQFIVERHSTGGRTIIAGYPWFSDWGRDSLISLPGLTLVPGRFDEARAILRTF
                     AQSVRDGLVPNCFSDVGSEALYNSVDASLWFFFAVYKFLEYTDDEPFVLEHLAEAMQA
                     ILDGFMNGTRFHIRMDPDDGLITAGETGVQLTWMDAKVDGLVVTPRQGKPVEVNALWY
                     NALRIFELVQNRAGNDTREISALARKVRISFRKTFLNEDMGYLYDVITPEGTPDHTFR
                     PNQIFAVYLPFSLLEPHQEKMIVDAVFKRLYTSYGLRSLMPKDPQYLGMYRGDRLHRD
                     KAYHQGTVWSYLIGPFLTSFLKVHHHSMEAQLRASYMLEPLRTHLEREACLGSISECS
                     TATRHIRRRAASPRPGASPRCCAATSKTSRASARTRCRSRQPRLHSRSAAPGATVCTA
                     AFPAHTRSDLTWSGFGGNVTASWNDLNHCLLSPGPLPTSPT"
     gene            6877..7143
                     /locus_tag="PKO06_00545"
     CDS             6877..7143
                     /locus_tag="PKO06_00545"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNV68153.1"
                     /translation="MERPQSLSLVAGAFADVANLISLGVLAGTCIGFGLAAGWLVDYW
                     FSLHPYGILGGIMLGLASAVRESYRMLRHSIECFERRKQTAQSR"
     gene            7109..7525
                     /locus_tag="PKO06_00550"
     CDS             7109..7525
                     /locus_tag="PKO06_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68154.1"
                     /translation="MNDENKLLNPVEEKVRLLIKRVGVASGVLALGLFLIGLIFWKSL
                     WLGIPCGIAIGFGSFFVLAQTVNTLTAPTKIHALFMVILSSLKLVLLLLALWVCYKIG
                     LDMLQLIVGLFLSQLAIFSGIVLTFHRGQDKPAEIP"
     gene            7527..8204
                     /gene="atpB"
                     /locus_tag="PKO06_00555"
     CDS             7527..8204
                     /gene="atpB"
                     /locus_tag="PKO06_00555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNV68155.1"
                     /translation="MELLESKIFSFFHVLHLNPGVWAPLLVTAIWALIGYLISRHLEL
                     VPGRLQTGIELLLDSVHSILKPVLGPAHALELVPFFTTLFIYLLSANMIGLIPGLKSP
                     TSIFSNCLGMALIIFCATHVMGLYKKGFGYIAHFWGEPLWLGPLMFPLHIIGELVRPL
                     SLTLRLFGNIMGEDIVLLVLLVFTIPWVLPLPMMAFAIFTSVLQALVFTMLSTVYVSQ
                     AMAPDHH"
     gene            8237..8482
                     /gene="atpE"
                     /locus_tag="PKO06_00560"
     CDS             8237..8482
                     /gene="atpE"
                     /locus_tag="PKO06_00560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNV68156.1"
                     /translation="MDNLTVLHIGYAASLSMSLAVACSACALAQGIAVKSALESMARQ
                     PELAGDLRLSMIIGLAMIESLAIYCLLVSLILLFVRW"
     gene            8610..9257
                     /gene="atpF"
                     /locus_tag="PKO06_00565"
     CDS             8610..9257
                     /gene="atpF"
                     /locus_tag="PKO06_00565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNV68157.1"
                     /translation="MKRLILTVLPIVFLCLLIGSSASAEPAAEAPPTQSEAVRYPSTA
                     TKSAHEEGGMFDIDPKILVAQTVNFFLLLWLLNKLLYVPLAQLIEQRRAHIATDIDLA
                     RHERRQAETIRQEWEKKIETVDQETYRMRQQAISEANAAREQIIAEAKAAAASQKQAA
                     ERDILLEKQKAWVELREEVVQLSLAVAEKVIEKSLDDQTHRDLIRSTISQLEKTR"
     gene            9254..9877
                     /gene="atpH"
                     /locus_tag="PKO06_00570"
     CDS             9254..9877
                     /gene="atpH"
                     /locus_tag="PKO06_00570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNV68158.1"
                     /translation="MKTRVVARRYAEAFFQVIRESTLEEAYADFSGFMEAMRQSDEMR
                     AVLKHPAVKVERKTELLKSLLSSSTQPVVNEFLCLLLRRHRFVFIDLIAQEVRDLYRR
                     EKRIVRVLVRTAVPLLEPERQDLIERLKKQVQGTIELKEQVEPQLKGGMILCFSEKVL
                     DTSVKTRLKLLRERLAHLKSELLSSVQGVPALVASPGSAPTPSSPSH"
     gene            9887..>10291
                     /locus_tag="PKO06_00575"
     CDS             9887..>10291
                     /locus_tag="PKO06_00575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582288.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNV68159.1"
                     /translation="MPIKPEEILSVLKNEIKSFSTEMKVDDAGTVVQVGDGIARVYGL
                     PKVMAGELVEFPNGVKGYVLNLEEDNVGCILLGPDIGIKEGDLVRGTGKVVSVPVGDA
                     LLGRVVNALGEPIDGKGPIVATETRPIETPAPN"
BASE COUNT         2067 a         3204 c         2793 g         2227 t
ORIGIN      
        1 cgtcttggtg ctcttcatca atcgcgggtt gaagctccta cagtatattc cagcgcaagc
       61 actcgacaag acctatctgg cgcgtgtcac gctggggcgc accaccgaca cctacgacgc
      121 ctgcggtgaa acgacggccg ttcatccgca tccgatcaac gtcacgaaag aacaaattct
      181 cgagaccctg cggggctttg tcggtgagta tctgcagaaa cccccttcct tctccgccgt
      241 gaaggtcaaa gggaaacggg cctatgatct ggcccgacag ggagttgccc tcgatcttcc
      301 cgagcgcccg gttcacgtgt catccattcg tttgattcgc gactacgaat caggcggatc
      361 acgccatctg gttctgcgca tccactgttc acgtggcacg tatatccgat cgctcgcgca
      421 cgaccttggg cacacgctcg gctgtggggc gcatttgtcc tacctgcttc gtgagcgggt
      481 cggggtttgg tcgattcatc aatcgattgc gccgcgcgtc atcgagcgtc gcgagtcggt
      541 tctcgccctg tccggcttca tgcccctcga tcgcattctt ccctaccccc gcgtggtcat
      601 caaccacgaa gcggaagccc gcatcagaca tggaaacccg ctcgatcccc gcgacatccg
      661 atccatctcc gcggaacatg tgtcgactcc cgaagaagca gacgaacaga gtctggtgca
      721 ggtgttttct gagaacggag agtttctcgc gctctataaa cccgatcgca ctcgtgttga
      781 acgcggtcaa ggccgactcc aaccggtacg cattctggtg tgacatgaaa ctggtcaatt
      841 ttctcggcgg ccacattccc tttggcccct gcgttctcgg cctgggaacg ttcgacgggt
      901 ttcacctggg tcatcgtgaa ctggtacgcc gcatggtatc gagagcccgg gaactgcagc
      961 tgcctgccgt gctgttcacg ttcgacctgc ctccgaagaa gattctcgat ccccagaact
     1021 ttccgggggc tctgacgctc ccggaagaga agttccaact gttgctcgag tctggcgtcg
     1081 actgggtggt ctttcgcacc ttcgacaagc agtttgccgt cacctattct caggagttcg
     1141 tgcgtcgcat tctggcagag aaactgcagg ctcgcgaggt ttttgtcggc ttcaacttcg
     1201 ggttcggctt caaccgcgag ggaaccgccg attcactggc gaccgacctg cacgaactcg
     1261 gcatcgggtg tcagattctg ccccgggtca gcatcgacgg agtgacgatc agcagcacct
     1321 ttattcgcga acagatcaaa agcggcaatc tcgatcaggc gaccaaatgg ctcggtcgac
     1381 cggtcaccct gaccggaacc gtgattcacg gcgagaaacg cggacgccag atgggctttc
     1441 ccaccgcgaa tctctcgctc gaacatccgg tcaaggttct gccaccgcat ggggtctacg
     1501 acactatcgc cgacacgccg ttcggcagct accaggccat gaccaatatt ggggttcggc
     1561 ccacgtttgc ccgacacacc ccacttctcg aagcgcattt gctcggcttc aacggcgacc
     1621 tctattcgca gaccatccgc gttcgcttct accgccggct gcgcgaagaa cggtgttttc
     1681 cgtccatgga gtcactgatc gcacaaatga aatgcgacct cgaagaagtg cgcacttcct
     1741 ccatgacacg tcggggacac ccacgtgaca tggggcaatc gtcctagaca aacggcaatc
     1801 ggtctcgtca atcgttctcg tcaaaggtca cttaggccac tggcgcaaga tcggcgagat
     1861 tcggcccggt cgcggcatcg cacaccaggg gcacgtcgag tgccgcaacg cgttccatgg
     1921 ttgtcgtcat gagatcgctc accgccggca gttcttcggg cggcgcggaa aacaccagtt
     1981 cgtcgtgaat ctgcagaatc agtcgggtgc ggaatccgcc gtcgcaaagc gctttcgcgg
     2041 catcgagcat ggctttcttg accagatcgg ccgccgtgcc ctgcagcggc gtattgcgag
     2101 ccactcgttc gccgaatgct ctctgattgc ggtttcggtc agcgagttcc ggtaacggcc
     2161 gcactcgccc ccacagggtc gacacctcca ttttctcggc cgcctcctgc tgattgcgct
     2221 cgaaaaacgc cgtcaccccc gggtacccgg caaaaaactg atcaatgtac gtctgagcgg
     2281 cattgcgggg aatctggagc tgacgagcga gaccatgtgc cgagatccca tagatgatgc
     2341 cgaaattcac agtcttgccg accttgcgtt cctcatcggt caccgcctcg gggaacttgc
     2401 cgaacaggcg acctgccgtc accgcgtgta catcgacccc gtgacgaaaa gcttcgacga
     2461 gggaagggtc tttcgagaaa tgcgccagca agcgcaactc gatctgcgaa tagtcgatag
     2521 acagcagcgt ctgccccgca acaggtggga caaacgctcg acgcactttc cggccccatt
     2581 cggacttgat cgggatgttc tgcagattcg gattggaact ggacagtcgc cccgtcgcgg
     2641 tgatcgtctg attgaaatgc gtgtgaatca gccccgtgcg ttgatcaacc aggtcagcca
     2701 gcgtgtcagc atacgtgttt ttcagcttgg ccagctcacg gtattcgagc agcaactgcg
     2761 caatcggatg tgcatccgcc aggtgttcca gcacttcgct gtccgtcgac aaccccgtac
     2821 gcgtgcgttt gggcgaaggc aatgccagct tctgaaacag cacttcctgc agctgcttgt
     2881 tggaattcac gttgaacgga ccgccggcat actcgtgaat acgctgttcg agctggttca
     2941 gacgtgactg cagatctctc tgcagctcgc cgagatagct ccggttcaac ccgatgccgg
     3001 tggtttccat ttcgagtaac agcggcatca ggggacgttc gagttgctca tacagtttcg
     3061 ccagctctgg tcgcgccgaa aaacccgccg cgaacttctc gcgcagcagc cacggcagaa
     3121 tcacgtcctg gccggcatac tccgcggctt tgtccaaagg cacatcagca aaattccctt
     3181 tgccgaccac ctgttcaaag gtcaccgcgt ccaggccgag ttctgttctc gccagctctt
     3241 tgagagcatt cgaacgtgtc ggattcaaaa catacgccgc cagcatcgta tcttctggcg
     3301 tgcccgtgcc cgtcacccct tcgcgatgca ggaacgccag atcgaacttc aagttgtgcc
     3361 cgagaagccg tcgggtctgc aaagcctcgg caagccgcgg aaacaccagc gccgcgtcgg
     3421 gttgcccttc ggcctgaaat ccgaaatacc ggtgtcgcac cgggacatac caggagcgct
     3481 cagggccaca ggaaagagag aacccgacga tacgcccctg acgaggcgac agatggtcag
     3541 tttccacatc aatggcaacc gtttcgccca gctgcgaaag cacctgttca aactcttcgt
     3601 cggtgcgtac tagcagcctc tcgcccggga ctggctgcag cactggttcg accatgacgg
     3661 ttggcgcgcc gacttcgcca ctcggttcat cgagaccagg caaagagggc tcgtgcggct
     3721 ggcccccggg gcgcgtctcc tcctgaaccg gcttccaggc gggggaatca ggatactgcc
     3781 gttcgagttc ctgcaccatc gagagaaagt tccagcgacg cagcagcggc accagagccg
     3841 gcaaggcggc ttcaagtccg ggccaggcga ggtcttccgg gccggtaccc cagggggcat
     3901 cacgcttgat ggtcgccaga cgatacgaaa aataggctga ttcacgcccc tcgatcaact
     3961 gctgcttcat gcgcgggttc gcgatctgct cgagcgcggc atacaccccg tcgagcgagc
     4021 cgtgttgctg aatcaacgtc gtggccttct tttcgccgat ccctgtcacg cccggaatgt
     4081 tatcagacgc atcacccatt aacgccttat aatcaacgat acgaatcgga tcaaacttgt
     4141 actcttgcac aaatacttcg cgattcatca tcgttgtttc cgagagattg tgccgacaga
     4201 gcagcaccac cacctggtca tcgatcaact gcaacagatc acggtctccc gtgacaatgc
     4261 gcaccggcag gttccctttg cagctttcgg cggccgaacc aatcagatca tccgcctcgt
     4321 attcctcgct catcagcagt cggaacccga gcgcacgaca gagatcccag gcgtgcggaa
     4381 tctgtttcaa cagttcactc ggcgtcggac gacgggtact cttgtattcg gcatacatct
     4441 ggtggcgaaa cgtcttcctg gccatgtcga acgcgatacc gacgcactcg ggtctttcat
     4501 cgcgcagcac cttgagcaga atgcgcagaa aaccgaacag agcgcccgtc ggctcgccgg
     4561 accggttggt cagacgctgt ttctcgagcg catagaaggc gcgatacagc aggttgtttc
     4621 catccaccag cagacagccg gacatgtcga gctccttcac gattcaatct ccgcggatgc
     4681 ggcgtcagca gtttaccttt ctctcccgat tgattcaacc ggccgtatgg tatacttccg
     4741 ccgcatcaac acccggaggc agaacatgct atcgatcgga cgcgatattc tcggcaacat
     4801 caaggaagca gcccgcctcg aatggctggt caccaacggc atcggcggtt tcgcctcttc
     4861 ttcggttctc ggcatgaaca ctcgccggta tcacggcatt ctggttgcag ctcgtcgccc
     4921 tcccaccgaa cgcctcgcga tcgtttcgcg cgtggaagag agcttatgtg ttgatggcca
     4981 cgaggttttc ctttcaacca accggtacca gaaagccttg tacccggccg ggtatctcca
     5041 tctcgagcgt tttgaccgcg atccgctccc gacctggacg ttccaatatg aagatatact
     5101 ggtggtgaag accctgttca tggtccacgg ggaaaacacc acggtcatca cctacaaggt
     5161 gttttcgaac ggtcgcgatg tcgaactgcg tctgcgcccg catctgttgt ttcgcgattt
     5221 tcacggaaac acgttcgaga acccgggatt cgaagacaac acgaccgtcg tcgacacttt
     5281 cttttcgctg caacctttcg ccaacgcccc tgcgctgtat atgtcgtggg accgtggcac
     5341 cttcgtgcgc gaagcgttct ggtatcgcga caaccacctg atcgaagaag agaaccgtgg
     5401 tctgaacgcc ctcgaagacg attattccaa cggctatctg cgagtgccca agtgtcaggg
     5461 aacggcgtcg atctgttttt ctctgacgcc cccggtcaag cacgccaacg ccctcgaact
     5521 gcgcaagcgc gaggaactgc gccttgaaag tattgcgcag agcctgcagt cggaagatca
     5581 gttcctgcgg cgtctcttgc tcgccgctga ccagttcatt gtcgaacgtc acagcaccgg
     5641 cggtcgcacc atcattgccg gctatccctg gttctccgac tggggacgcg acagcctgat
     5701 ctctctgccc ggactgacgc tcgtgcccgg gcgcttcgac gaggcccgtg ccattttgcg
     5761 caccttcgcc cagtcggtgc gcgatggtct cgtgcccaac tgcttttcag acgttggcag
     5821 cgaagctctc tacaacagcg tcgatgcctc gctctggttc tttttcgccg tgtacaagtt
     5881 cctcgaatac accgacgacg agccattcgt gctcgaacac ctcgccgagg ccatgcaggc
     5941 cattctcgac ggcttcatga acggcacgcg ctttcacatc cgcatggacc cggacgacgg
     6001 tctgatcacc gcgggtgaaa caggcgtaca actcacctgg atggacgcca aagtcgacgg
     6061 cctggtcgtg actccgcgtc aaggaaaacc cgttgaggtc aacgcgctct ggtacaatgc
     6121 gttgcgcatt ttcgaactgg tacaaaaccg agccggcaac gacactcgcg agatcagcgc
     6181 gctcgcccgc aaggtccgca tctccttccg gaagaccttt ctcaatgagg atatgggcta
     6241 cctctacgac gtgatcacgc ccgagggcac acccgatcac accttccgac ccaaccagat
     6301 cttcgcggtc tatctgccct tctcgctgct cgaaccacat caggaaaaga tgatcgttga
     6361 cgcggtcttc aagcgcctgt atacgtccta tggtctgcgc agcctgatgc cgaaagaccc
     6421 ccagtatctc ggcatgtacc gcggcgatcg cctgcaccgt gacaaagctt atcaccaggg
     6481 aaccgtctgg tcgtatctga tcgggccttt tttaacgtcg tttctcaaag tgcatcacca
     6541 cagtatggaa gcccagttgc gcgcttcata tatgctcgaa ccgctgcgca cgcaccttga
     6601 gcgcgaagcc tgtctcggct cgatctcaga gtgttcgacg gcgacccgcc acatacgccg
     6661 aagggctgct tcgcccaggc ctggagcatc gccgaggtgc tgcgctgcta cgtcgaagac
     6721 atcaagggcc agcgcccgaa cccgctgtcg tagccggcaa ccacgcctcc attcccgttc
     6781 ggcggccccg ggagcaactg tttgtaccgc tgcctttccg gctcacacac gatcggattt
     6841 gacttggagc gggttcggtg gtaacgtgac ggcttcatgg aacgacctca atcactgtct
     6901 cttgtcgccg gggcctttgc cgacgtcgcc aacctgatct cgctcggagt tctggctggc
     6961 acatgcattg gtttcggcct cgctgccggc tggctggtcg attactggtt cagcctgcat
     7021 ccgtatggca ttctcggcgg catcatgctc ggtctcgcct cggccgtccg cgaatcgtac
     7081 cgcatgctgc gtcacagcat tgaatgcttt gaacgacgaa aacaaactgc tcaatcccgt
     7141 tgaagaaaag gtcagactgc tgatcaagag agtcggcgtg gcttccggcg ttctcgcact
     7201 cgggctgttt ctgatcgggc tcattttctg gaaaagcctg tggctgggca ttccgtgcgg
     7261 catcgccatc ggattcggca gttttttcgt gctggcccag acagtcaaca cgctgactgc
     7321 tccgacaaag attcacgcgt tgttcatggt cattctctcg agtctcaagc ttgtgctgct
     7381 gcttctcgcg ctctgggtgt gctacaagat cggtctcgat atgctgcagc tgattgtcgg
     7441 cctcttcctg agccagctgg ccatcttttc cggcatcgtg ctgacctttc atcgcgggca
     7501 ggataaaccc gccgagatac cgtaacatgg aactcctcga atccaagatc ttctcctttt
     7561 tccatgtcct gcatctcaat ccgggtgtct gggcaccgct tctggttacg gcgatctggg
     7621 cactcatcgg atatctgatc agtcgccatc tcgaactcgt tcccggccgc ctgcaaaccg
     7681 gaatcgaact cctcctcgac agcgtgcact cgattctcaa gcccgtactt gggcccgcgc
     7741 atgcgctcga actcgttccc ttcttcacga cattgttcat ctatctgctc agcgcgaaca
     7801 tgatcgggct cattcccggc ttgaaatcac cgacgtcgat cttctcgaac tgtcttggaa
     7861 tggcgctgat catcttctgt gcgacgcatg tgatgggttt gtacaagaaa gggttcggtt
     7921 acatcgcaca cttctggggg gaaccgctct ggctcggccc cctgatgttc ccattgcata
     7981 tcatcggaga actggtgcgt cctctcagcc tcactctgcg cctcttcggc aacatcatgg
     8041 gcgaagatat cgtcctgctc gtactgctgg ttttcaccat tccgtgggtt ctgccactgc
     8101 cgatgatggc ttttgccatc ttcacgtccg tgctgcaggc tctcgtcttc accatgcttt
     8161 caaccgtgta cgtcagtcag gccatggcgc ctgaccatca ctgatagcgt agtctcgcga
     8221 ttcggaggaa cgaacgatgg ataatctcac agttctgcac atcgggtatg ccgccagtct
     8281 gagcatgagt ctggctgtcg cttgcagtgc ctgcgctctg gctcaaggca ttgccgtgaa
     8341 gtccgcgctc gaaagcatgg cgcgccagcc cgaactcgcc ggcgacctgc gtctgagcat
     8401 gatcatcggt ctcgcgatga tcgagtcgct ggcgatttac tgtctgctcg tttctctcat
     8461 tctgctcttc gttcgctggt gagcttccgc tcaacggtgc ccgccatcag ccgactcgcg
     8521 cgggcgtcta agagaccagg tccgagttct cgcgatccgg aacgaactcg ctctttccgc
     8581 gttcacccac gctttcgggg gttgaaacca tgaaacgcct cattctcaca gtgctgccga
     8641 tcgtttttct ctgtctgctc atcggctctt ctgcctcagc tgaacccgct gcagaagcac
     8701 cgccaactca aagtgaagcg gtacgatatc catcgacggc gaccaagtcc gcgcatgaag
     8761 aaggcgggat gttcgacatc gatcccaaga tcctggtcgc ccagaccgtc aacttcttcc
     8821 tgctgctgtg gctgttaaac aagcttctgt atgttccgct ggcccaactg atcgaacagc
     8881 ggcgggccca catcgcaacc gatattgatc tggctcgcca tgagcgtcga caggcagaaa
     8941 ccattcgaca ggaatgggaa aagaagatcg agaccgtcga tcaggaaacg taccgcatgc
     9001 gtcagcaggc tatcagtgag gccaacgccg cccgcgagca gattatcgcc gaggccaagg
     9061 ccgccgctgc gtctcagaag caggccgccg aacgagacat tctgctcgag aaacagaaag
     9121 cctgggtgga actgcgtgaa gaggttgtgc agctctcgct ggccgtcgcc gagaaggtca
     9181 tcgaaaagtc cctcgatgac cagacccacc gcgatctgat tcgcagtacc atctcacagc
     9241 tggagaaaac caggtgaaaa cccgggttgt cgcccgtcga tatgccgaag ccttcttcca
     9301 ggtgatacgg gaatcgaccc tcgaagaggc gtatgctgac ttcagcggtt tcatggaagc
     9361 gatgaggcag tcggacgaga tgcgcgccgt cctgaagcac cccgccgtca aggtcgaacg
     9421 caagacggag ctgctcaaat ccctgctttc ctcttccact caacccgtgg tgaacgagtt
     9481 tctctgtctg ctgctgcgtc gccatcgctt tgtcttcatc gatctcatcg cccaagaggt
     9541 cagagacctg taccgccggg aaaaacgcat cgtacgggtg ctggttcgca cggccgtacc
     9601 attgctcgaa cccgagcgcc aggatctcat cgagcgactc aaaaagcagg ttcaaggaac
     9661 catcgaactg aaggagcagg tcgaacctca gctcaagggc ggcatgatac tgtgtttctc
     9721 cgagaaagtc ctggatacgt ccgtgaagac gagactgaaa ctgctgcgcg agcggctggc
     9781 acacctgaag agtgaactgt tgtcgagcgt tcagggggtg ccggcgttgg tcgccagccc
     9841 cggttcggca cccacgcctt cgagtccttc gcattgagga gtcgttatgc ccatcaaacc
     9901 tgaagaaatt ctgagtgttc tgaaaaacga gatcaaatcg ttttcgaccg agatgaaggt
     9961 cgatgacgcc gggacggttg tacaagtcgg cgacggcatc gcgcgcgtct acgggctacc
    10021 taaggtcatg gccggagaac ttgtcgagtt cccgaacggc gtcaagggct atgtcctcaa
    10081 ccttgaagaa gacaatgtcg gttgcattct gctcgggccc gatatcggta tcaaggaagg
    10141 cgatctcgta cgcggcaccg gcaaagtcgt ttctgtacct gtcggtgacg cgttactcgg
    10201 gcgtgtcgtc aacgctcttg gcgaaccgat cgacggcaaa ggtccgatcg tggcaaccga
    10261 gaccagaccc atcgagacgc cagccccgaa t
//
LOCUS       DAORHD010000011        10257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_5823_length_10257_cov_3.186630, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000011 DAORHD010000000
VERSION     DAORHD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 10257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10257
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5823_length_10257_cov_3.186630"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKO06_00580"
     CDS             <1..669
                     /locus_tag="PKO06_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV68160.1"
                     /translation="HWSGSYGEDFGDATTHATELNVFESFDPKLPESYRNVPYLFLAN
                     IHPALQLKVIEKVARPKLIALDTMNFWISSFVDDLKKVIQKVDVLFLNRQEAMQLSGE
                     KKLVQAYQKLLTLGPKRLVIKLGELGAMTVTKDSTFFVPAFPCPGITDPTGAGDSFGG
                     GFMGHIAQSDDLSENGFRRAMLYGSTRGSFTVENFSIDTYRQLKRADIDARYHKLMES
                     IAVK"
     gene            complement(755..1351)
                     /locus_tag="PKO06_00585"
     CDS             complement(755..1351)
                     /locus_tag="PKO06_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68161.1"
                     /translation="MTLRQHVSMCLVIAVCLFLPCEKSYLKAEEESVELYYDMLESDD
                     ETVRENGVRLILAREPKNRKALENYLELEEERGNYSEAIEWLKRLEGAGVLSKDQAEE
                     RCNRLQERLANQEHISIWENRLDRFKQKEQYSLALHEVRKLKAWYSARKTKAEARMPD
                     LNKESGEYLDSESQRKNAKYYLSLYEETEKEILERLKN"
     gene            complement(1357..2166)
                     /locus_tag="PKO06_00590"
     CDS             complement(1357..2166)
                     /locus_tag="PKO06_00590"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNV68162.1"
                     /translation="MATAPVRAEIGVFGGSGFYSLFSHPAEVKVDTPYGPSSDLVTLG
                     EVRDKVVAFIPRHGRKHTIPPHMINYRANLWAMKELGVTRIIAPFACGSLQTNLRPGD
                     FVVVDQFVDRTCGRADTFFNGPKVSHISSAEPYCPELRQLAVQVLKSNGITTHDGGAV
                     VVIQGPRFSTKAESRWFTKCGWELVNMTQYPEVVLAQELGMCVTGIGLVTDYDAGLAG
                     NVPEVTAAEVARTFHQNSENLRRIMEPLIAAIPAERKNCRCAEKPAEAMLG"
     gene            2266..2694
                     /locus_tag="PKO06_00595"
     CDS             2266..2694
                     /locus_tag="PKO06_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV68163.1"
                     /translation="MVKHLRVAQREQCIGCYSCMYSCSRTWSNVMSPEKAALRVRNYS
                     GAEGAFSIRACFGCTDPDCVKPCPTQAVTVKSGGGIEFHADKCIHCGKCVTACIPKAH
                     QWDAEKRVPIPCRHCGICVSFCPNCVLEMQPREELKGAGA"
     gene            2691..4451
                     /locus_tag="PKO06_00600"
     CDS             2691..4451
                     /locus_tag="PKO06_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNV68164.1"
                     /translation="MTSFRMLEINLTTGSSTTTDISALFAEYLGGTGVATRLLLDSPS
                     DIDPYAPEAPIYFAIGPFSSVLPVATKTVALFKSPLTGNLGESHAGGRLALAMYGAGI
                     HVLKITGACTELSYLVIHDDKITIRRATSLKNMSALATERVLREREDSQSKRSIVRIG
                     PAGERRSPMAAVTVDGSRHFGRMGLGGVMGSKNLKAFVISGSHYWTIENKAKYNALYK
                     KLYDVVVKSETMHKYHDLGTAMNVVPLNQINGLPTRNFSQGYFESAHQISGETFADKH
                     LAQQTACAHCPTGCIHIATLRECFDVDDHHFKTFKVSYDHELIYALGSNLSIQNAEDI
                     LKLLLIIEKQGWDAISMGVTLAWACEAFQHGLITPKHTDGLIFNFGDAFGLEKAMQHI
                     AAGKNEFFRDLERGASYCAAKYGGTEFAVAFGKTEAAGYATGPFAFLGYATGTRHSHL
                     DSAGYSIDQKAISKPKPDEDLVKDMYKEAVWRMIPASLVICFFARNVYTKEVIVEGLD
                     ALGIPGWNDTRLDALAKRIHAMKFRYKLNCGFRWEDLTFPKRLEQLTTSMGQVTCMRF
                     GENIARFRQIVETDLQQLSL"
     gene            complement(4505..5536)
                     /locus_tag="PKO06_00605"
     CDS             complement(4505..5536)
                     /locus_tag="PKO06_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV68165.1"
                     /translation="MLGRLFPLSQSERLRFLRQFYVILQASLPLPDLIRRLAHIEESP
                     VLRDCLRVTGIELERGASLTMIWCRFPHLFPDSLLQYLRLGEESGQLAAALQEYLDFA
                     HRADQHRLIVWSAGAVVLAIAFGVGFILYCSGTPLMTLWELATPYSLVPIHVSINLWF
                     SPDLILLALAVLWLPTRLLLAKVSLKITHLRVSRLWLGVPALGAAFCKNDLARFLRAI
                     RIGLVSGLPIASAGRCAAETVENRYLADELGLVVRGLQTGLTLSQAFSMVSIPMPCLR
                     LALEAAEEGGELDVVLAGQADVYVREAESVFRWATTVLEPLAGLCGLAVMGGAGMLLL
                     RLMTGVAAL"
     gene            5766..6746
                     /locus_tag="PKO06_00610"
     CDS             5766..6746
                     /locus_tag="PKO06_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68166.1"
                     /translation="MRPHEIENWVLQIVERVKVRQPIEDSRVELKSAWIKPDKAARQI
                     AGHANSAHGSPVLWLIGLDEVNGVVGASHEELAEWYAQVKSQFNGLAPALMHYNIPID
                     DRTVVALLFESDRAPFVVINPMFGKTGGGSVALEVPWRENTSTRTATRAELLKILAPH
                     QELPIFDILEGQLVARPEKHNGQETLTWTLNLTLYAETASTTRVVIPFHKCAVSLELP
                     GILPISEFTSFSMTPPEQVRIREMTATPMSLTIEATPDEILINGPGKLHLTGQMYCSV
                     PQPIPDELPVQIKASLRPVLSDISIPLHLTLIRVKPDVGKVMKWSFRARS"
     gene            complement(6828..7562)
                     /locus_tag="PKO06_00615"
     CDS             complement(6828..7562)
                     /locus_tag="PKO06_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68167.1"
                     /translation="MMTLTMRRRQPISLLLVLTMFLSTFLVAPATSEAGLMDSVKSVS
                     KWLFVKAGSLGAAYGGAVLGMAIGGGPIGMVLGGVGGYIVGKKVMEWGTSNSKNFWTL
                     AGGLAGAALTVGMGWPVMLAGVAVGALGSRALASVIGKIFNKAKAVAAPVTAQAAAKM
                     ADQDAKAQAFIDQMMGKANGSSGTTAAQAAVAPMTAGTGSQGAYDRYLSAYKTYMDAT
                     QKGDAQAAQQAYTEYQSALKEYRGGK"
     gene            complement(7647..8651)
                     /locus_tag="PKO06_00620"
     CDS             complement(7647..8651)
                     /locus_tag="PKO06_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV68168.1"
                     /translation="MSCRRLLAVFLVLAVVGVVAPVGAAGLRVGVVLSLGGLGDRSFN
                     DAAYLGVQKLREIPGCQVDVLEPSDIEAIAAGLEFFCQRRCDLIIGVGLFANEPLRRV
                     AQQYPQQSFALLDSVVTLPNVLSIIFNEEEGSFYAGALAGLLTRTNRVGFLGGMKSPV
                     VNGFERGYKRGVRFVNPQAELVVRYAGETPEAFNSPKLGETLGGEIAAEKADIVYHAA
                     GRTGMGLIQSARRHGYLVIGVDSDQNAIVPGKIAASMVKRLDVAMDRAARLIMESRFK
                     GCVLTLGLVDGGIDLTLSKFNRALFTPILQERLKEVEQFLLHPPATTPDEPGAVENPR
                     "
     gene            complement(8730..9698)
                     /locus_tag="PKO06_00625"
     CDS             complement(8730..9698)
                     /locus_tag="PKO06_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV68169.1"
                     /translation="MKPLELSSVAEVWKSALTILEKALEPVGCQVQVWFPWGKDFRGH
                     DLAGSLPPTELPPDLASPQWARYAGRHWRSLNVAHNQWLISIKSKSGDLAASGRIDLE
                     LTPEIQNTFAMLGEFVKMECLLGLSLRILEARAGERTGHWERVRNLSVAVGRKMGLVP
                     RELVDLELTGLLHDIGKIALPASLWEETRPLSAEERRQMETHSAAGAAMIREIPGLDR
                     VAVGVHCHHEAPDGSGYPKGLTAEEIPLNALIVGAVDAFDAMTHYRPYAAERTYKDSI
                     QEMMTASGKYDDRVLWALQEVLRSLGILDTYPVSPAHPRYSDGPVA"
     gene            9973..10239
                     /locus_tag="PKO06_00630"
     CDS             9973..10239
                     /locus_tag="PKO06_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNV68170.1"
                     /translation="MPKHPTHPDLTRRLARIAGHVEAIRRMLDEERSCPEILQQMKAV
                     LAALENARRILAEDHIRHCLVDAIERKNTKAVIEEIEEVFSQIL"
BASE COUNT         2359 a         3111 c         2676 g         2111 t
ORIGIN      
        1 cactggtccg gttcttacgg tgaggatttt ggcgatgcca cgacacacgc gacggaactc
       61 aacgtctttg agtcattcga ccccaagctc cccgaatcct accgcaacgt tccgtacctg
      121 tttcttgcga acattcaccc agcgctccag ttgaaagtca tcgaaaaggt tgcccgcccg
      181 aagctgatcg cactcgacac aatgaacttc tggatctcga gcttcgtcga tgatctgaag
      241 aaggtcattc agaaggtcga cgtgctgttt ctcaaccgcc aggaagccat gcagctcagc
      301 ggagaaaaga agctcgtgca ggcttatcag aaactgctga ctcttggccc caagcgtctg
      361 gtcatcaaac tcggcgaact tggcgcgatg acggtcacca aagactcgac cttcttcgtt
      421 ccagcctttc cctgccccgg catcaccgac ccgaccggtg ccggcgactc attcggcggc
      481 ggcttcatgg gtcacattgc gcagtctgac gatctgtcgg aaaacggttt cagacgtgcc
      541 atgctctacg gatcaacgag gggctcgttc accgtcgaga acttcagcat cgacacctat
      601 cgccaactga agcgcgccga catcgatgcc cgctatcata agctgatgga aagcatcgcc
      661 gtaaagtagg aagtacaaac tcacaacacc ttacagcatt atcgggggct cagaagagcc
      721 cccgatattt tttttgaatc ccatcgggcc gcgatcagtt cttcagtcgt tcgaggattt
      781 ccttctcggt ctcctcatac agactgagat agtatttcgc gtttttccgc tggctctccg
      841 agtccaggta ttcccctgac tccttgttca gatccggcat tctcgcctct gctttcgtct
      901 tcctcgcaga gtaccaggct ttcagttttc gcacttcgtg aagggcgagt gaatactgct
      961 ctttctgttt gaatcgatcc agacggtttt cccagatact gatgtgttcc tgattcgcca
     1021 gtctctcctg caaacgatta cagcgctctt ccgcctgatc tttggacaac acgcccgcgc
     1081 cctcgagacg tttgagccac tcgatggctt cgctgtaatt gccgcgctcc tcctcgagct
     1141 caagatagtt ttccagcgct tttcgatttt tcggttcacg cgccagaatc agccggacgc
     1201 cattctcacg taccgtctca tcgtccgact cgagcatgtc atagtacaac tcaacagact
     1261 cttcttcggc cttcagataa gatttttcac agggaagaaa caagcagacc gcgataacca
     1321 agcacatcga tacatgttgt ctcagagtca tgacgatcac ccaagcatgg cttcagcggg
     1381 cttctcagca cagcgacagt tctttcgctc cgccggaatc gcggcaatca gaggctccat
     1441 gatgcggcgc agattttcgc tgttctgatg aaaggtgcgg gccacttccg cggcggtcac
     1501 ttcgggcaca ttgccggcca aacccgcgtc ataatcggtg accagaccaa tcccggtgac
     1561 gcacattccc agttcctgcg ccagaacgac ttcggggtat tgggtcatgt tcacgagttc
     1621 ccagccacac ttggtgaacc agcgactctc ggccttcgtc gaaaaacgcg ggccctgaat
     1681 caccaccacg gctcctccgt cgtgagtggt gatgccgttg ctcttcagca cctggaccgc
     1741 cagttgccgc agttcgggac aatacggctc tgccgaagag atatgcgaca ccttcggtcc
     1801 gttgaaaaag gtatcggctc ggccacatgt gcggtcgaca aactgatcga caacgacaaa
     1861 gtcaccgggg cgaagattgg tctgcagaga cccacaggca aacggggcaa tgattcgggt
     1921 gacgcccagt tctttcatgg cccagagatt agcccgatag ttgatcatgt gcggtggaat
     1981 cgtgtgtttg cggccatgac gtgggataaa ggcgacaacc ttatcgcgaa cttcacccag
     2041 agtgaccaga tcactcgacg gcccatacgg cgtatcgact ttcacttctg ccggatgtga
     2101 aaacaatgaa tagaagccgc tgccgccaaa aaccccgatt tccgctcgca ccggtgcagt
     2161 tgccatggtg aatccccttt cccactgatt gattcgagcc atggtagcac gagcttcacc
     2221 gaaaatggta acctgcacga caccaaccgt acgaggggaa caatcatggt taaacatctg
     2281 cgtgtcgcgc aacgtgaaca gtgtatcgga tgctattcct gcatgtactc gtgcagtcgc
     2341 acctggtcga acgtcatgtc accagaaaaa gcggccctgc gggtgcgaaa ctactcgggt
     2401 gccgaagggg ctttttccat cagagcctgc tttggctgta ccgacccgga ttgcgtaaag
     2461 ccgtgtccca cgcaggctgt cactgtgaaa tccggtggtg gcattgagtt tcatgccgac
     2521 aagtgcattc actgcgggaa gtgcgtgaca gcttgcattc ccaaagcaca ccagtgggat
     2581 gcggaaaaac gggttccgat tccctgccgt cattgcggaa tctgtgtttc cttctgtccc
     2641 aattgcgttc tggagatgca accccgtgag gaactcaaag gagcaggcgc atgacttcct
     2701 ttcgcatgct tgaaatcaat ctgaccaccg gttcctcgac gacgactgat atcagtgctc
     2761 tgttcgccga atatctgggc ggaaccggcg ttgcgaccag actgcttctc gactcgccgt
     2821 ccgacattga tccctatgcc cctgaagcgc cgatctactt tgccatcggt cccttcagct
     2881 cggtactgcc ggtcgccacc aagacggttg ccttgttcaa atcgcccctg accggaaatc
     2941 ttggagagtc gcatgccggc ggccgtctgg ccctggccat gtatggcgct ggcattcatg
     3001 tattgaagat caccggcgct tgcacagagc tcagttatct ggtgattcac gacgacaaga
     3061 tcacgattcg gcgtgcgact tccctgaaaa acatgtctgc tctggctacg gaacgcgtcc
     3121 ttcgtgagcg tgaagactcg cagagcaagc gttctatcgt gcgcatcggt ccggccggcg
     3181 aacgtcggtc cccgatggca gcagtgaccg tcgatggctc acgccatttc gggcgcatgg
     3241 gcctgggcgg agtcatgggc agcaagaatc tcaaggcgtt cgtcatctcg ggcagccact
     3301 actggacgat cgaaaacaag gcgaagtaca acgctctcta caagaagctg tatgacgttg
     3361 tcgtcaagag cgaaacgatg cacaaatatc acgatctggg caccgcgatg aacgtggtcc
     3421 cgctgaacca gatcaacggc ctgccgacac gaaacttcag tcaagggtat ttcgaaagcg
     3481 cgcaccagat cagtggtgaa acttttgccg acaagcacct ggcacaacag accgcctgtg
     3541 cccactgtcc taccggttgc attcacatcg ccactctgcg cgagtgtttc gacgtcgacg
     3601 atcatcattt taaaaccttc aaggtctcgt atgatcatga actgatctac gccctgggct
     3661 caaacctctc gattcagaat gccgaagaca tcttgaaact cctgctgatc atcgagaaac
     3721 agggatggga tgccatcagc atgggcgtca ctctggcctg ggcctgcgaa gcctttcagc
     3781 atgggttaat cacccccaag cacaccgacg gcctgatctt caacttcggc gacgccttcg
     3841 ggctcgagaa agcgatgcag cacatcgccg ccggaaagaa tgaattcttc cgcgatctcg
     3901 aacgtggcgc ctcgtactgt gccgcgaagt atggcggcac cgaatttgcc gtggccttcg
     3961 gaaaaacgga agccgccggc tacgccactg gtcctttcgc ttttctcggc tatgcgaccg
     4021 gcacgcgtca ctcacatctc gattcggcgg ggtattccat tgaccagaag gcgatcagca
     4081 agcccaagcc cgacgaagat ctggtgaaag acatgtacaa agaagccgtg tggcgcatga
     4141 ttcccgcgtc tctggtgatc tgtttcttcg cccgcaacgt gtataccaag gaagtgatcg
     4201 tcgagggact ggatgcactg ggcatccccg gctggaacga tacccgcctc gatgcgctgg
     4261 cgaagaggat tcatgccatg aagtttcggt acaagttgaa ttgcggattc cgctgggaag
     4321 acctgacctt tcccaagcgt ctcgagcagc tcacgaccag catgggacag gtcacctgca
     4381 tgcgcttcgg cgaaaacatc gcccggttcc ggcagattgt tgaaaccgat ctacaacaac
     4441 tctctctata acaacgtctc gcaactcaac gacccggacg cttacttcgg gtcgttgttg
     4501 tttctcacag agccgccacg ccggtcatca atcgcagcaa cagcatcccc gcgccgccca
     4561 tgactgcaag accgcaaaga cccgccagcg gctcgagaac cgtcgtagcc cagcgaaaga
     4621 cactctcggc ttcgcgaaca tagacatcag cctgccctgc cagcaccacg tccagctcgc
     4681 cgccttcttc agcggcttcg agagccagac gcaggcaggg catcggaatc gacaccatcg
     4741 aaaaggcctg tgatagagtc agtccggtct gcagtcctcg gacaaccaga cccagttcat
     4801 cggcaagata acggttttcg acggtttcag ccgcacaccg gccggcgctg gcaatcggca
     4861 aaccgctgac aagaccgatg cgtatcgccc gcagaaagcg cgccaggtcg ttcttacaga
     4921 acgcagctcc cagagccgga actcccagcc acagacggga gactcgcaga tgcgttatct
     4981 tgagcgacac cttcgccagc agaaggcgcg tgggcaacca caacaccgcg agagccagca
     5041 gaatcagatc cggggaaaac cacaggttga tactgacgtg tatgggaacc aatgagtacg
     5101 gggtcgccag ttcccaaaga gtcatcaatg gcgtgccgga acagtacaag atgaacccca
     5161 ccccgaaggc aatcgccaga accacagcgc cggcactcca gacgatcagg cggtgctgat
     5221 cggcccgatg ggcgaagtcc agatactcct gcaacgctgc cgcaagctgc ccgctttctt
     5281 cacccagcct gagatactgc agcaatgaat ccggaaagag gtgcggaaag cggcaccaga
     5341 tcatagtcag actggcacca cgttcgagct cgatcccggt cactctcaaa cagtcacgca
     5401 acaccggact ctcttcgatg tgggcaagtc ggcgaatcaa gtccggtaag ggtaacgaag
     5461 cctgaagaat gacgtagaac tgccgtaaaa atcgtaaacg ctcgctctgc gagaggggaa
     5521 agagtcgtcc caacatacgt tcacctccat ctttcgcttc ggcgataccg cactcacttc
     5581 tgtcttcacc atggcaccgc atggaacatg ccattgctcg atcagctcgc agagactcca
     5641 ggcaagccag ttgtccgcac agctgcgcag gtttccgcac agcctgcgtt tcgaattgca
     5701 catccccaca tgccggagat cggcatttat ggtagagtat gatcaattct ctgagaggtc
     5761 cgtctatgcg tccgcacgag atcgaaaact gggttctgca gattgtcgag cgcgttaaag
     5821 tacgtcaacc catcgaagac tcgcgcgtcg agctcaagag cgcctggatc aaaccggaca
     5881 aagccgcccg tcagatcgcc gggcacgcca actcggcgca tggttcaccg gtactctggc
     5941 tgatcgggct cgacgaggtg aacggggtcg tgggcgccag ccacgaagaa ctggccgagt
     6001 ggtatgccca ggtgaaatcg cagttcaacg ggctcgctcc tgccctgatg cactacaaca
     6061 ttccgatcga cgatcgcacg gtggtcgcgt tgctgttcga atcagaccgc gctccgttcg
     6121 tcgtcatcaa tccgatgttc ggaaaaaccg gcggagggtc ggtcgccctg gaagttccct
     6181 ggcgcgagaa cacctcgacc cgcactgcca cacgcgccga gctgttgaag attctggcac
     6241 cgcatcaaga actcccgatc ttcgacattc tcgagggcca gctcgtcgcg cgtcctgaga
     6301 agcacaatgg tcaggaaacc cttacctgga cgttgaatct gaccctgtat gccgagacag
     6361 ccagtaccac tcgcgtggtc attccatttc acaagtgcgc cgtatctctc gaacttccgg
     6421 gaatcctgcc catcagtgag tttaccagct tctcgatgac ccctccggaa caagtgcgca
     6481 tcagagaaat gacggccaca cccatgtcgc tgaccatcga agccactccc gacgagattc
     6541 tgatcaatgg cccgggaaag ctgcatctga ccgggcagat gtactgttcg gtgccacagc
     6601 cgattcccga cgaactgccg gtccagatca aagcctcatt gcgcccggtt ctttctgaca
     6661 tcagtattcc tcttcatctc acgttgattc gcgtaaaacc cgatgtcggc aaagtcatga
     6721 agtggagctt ccgggcacgg tcctgaacca gtcgatcatt ccagaccacg agaaagcccc
     6781 cgctccggag agcgggggct tttcttcgag tcagatttcg aacgacgtta ttttccgccg
     6841 cggtattctt tcagagccga ttgatactcg gtatacgcct gctgagccgc ctgagcatcg
     6901 cccttctgcg tggcatccat ataggtcttg tacgcggaca ggtagcgatc atatgcgccc
     6961 tggctgcccg ttccagcggt catcggggcc acagccgcct gagcggcggt cgtaccagaa
     7021 gatccgttgg ctttgcccat catctgatcg ataaacgcct gagctttggc atcctggtca
     7081 gccatcttgg cggctgcttg cgccgttacc ggcgcggcca ccgctttggc cttgttaaag
     7141 atcttgccaa tcaccgaggc aagcgcacgg ctccccagag cgccaactgc caccccagcc
     7201 agcatgaccg gccaccccat accgacagtc agagccgcac cagccaaacc acccgccaag
     7261 gtccagaagt ttttcgagtt actggtaccc cattccatga cctttttacc aacgatgtac
     7321 ccaccgacac caccaagaac catgccgata ggaccgccac caatggccat accgagcacc
     7381 gcaccgccat acgctgctcc cagcgacccg gctttgacga acaaccactt cgatacggat
     7441 ttgacggaat ccatcaaacc cgcttcgctg gtggccggag cgacgagaaa ggtgctgaga
     7501 aacatggtca gcacgagcaa gagagaaatg ggctgtcgac gccgcatcgt gagagtcatc
     7561 atggtgctac ctccgcttcc agaaagggat cgcatacagt tatatccaat tataggtaca
     7621 atcggggcaa ccgtcaatga ccgcttctag cgcgggtttt ccacggcgcc cggctcgtcg
     7681 ggcgtggtcg cgggcggatg cagcagaaac tgctcaacct ctttcaatcg ttcctgaagg
     7741 atgggagtaa acagcgctcg gttaaacttc gatagcgtca gatcgatacc gccatcgact
     7801 aggcccaggg tcaggacaca ccccttgaag cgactttcca tgatcagacg agcggcacga
     7861 tccatcgcga catcaaggcg cttgaccatg ctggccgcga tttttcccgg aacgatggca
     7921 ttttgatcac tgtccacccc gatcaccaga tagccatgac gacgtgcgga ctgaatcagt
     7981 cccatcccgg tacggccggc agcgtggtag acgatatcgg ccttctcagc cgcaatctca
     8041 cctcccagag tttcgccgag cttgggtgaa ttgaacgctt caggggtttc accggcatat
     8101 ctgaccacga gctctgcctg cggattcacg aaacgaacgc ctcgtttgta gccacgctcg
     8161 aagccattca ccacgggcga tttcattccg cccagaaagc cgacccgatt ggtgcgggtc
     8221 agcaatcccg ccagagcacc agcgtagaat gagccctctt cttcgttgaa aatgatggaa
     8281 agcacgttcg gcagggtgac aaccgaatcg agcagcgcaa acgactgctg cggatactgc
     8341 tgcgccacgc gacgcagcgg ttcattggcg aacaatccca cgccaatgat caggtcacag
     8401 cgtcgttggc agaaaaactc gagacctgcc gcgatggctt cgatgtcaga cggttcgaga
     8461 acatccacct ggcacccggg aatttcacgg agcttctgca ctcccagata ggcggcatca
     8521 ttaaacgagc gatcgccgag acccccgaga gacagaacga ccccaacgcg caaccctgct
     8581 gcgcccaccg gcgcaaccac tccgacgacc gcgagaacca ggaaaaccgc gagcaagcga
     8641 cgacaactca tgaattctac ctcccgtaac tgggataaag agtgcaatga acgacctgac
     8701 agatgacacc gaaccgggac ccatactgct caagcaacag gaccgtcact gtagcgtgga
     8761 tgggccggag aaaccgggta ggtatcgaga ataccaagcg aacgcaagac ttcctgaagc
     8821 gcccacagca cgcgatcatc gtattttccg cttgccgtca tcatctcctg aatggaatcc
     8881 ttgtacgttc gctctgcggc atacggtcga taatgggtca tggcatcgaa cgcatcgacg
     8941 gcaccgacga tgagagcatt caaaggaatc tcttcggctg tcaacccctt gggatacccg
     9001 gaaccatcgg gagcctcgtg gtggcaatgc actcccacag ccacgcggtc gagaccagga
     9061 atctcgcgaa tcattgccgc accggccgca ctatgggttt ccatctggcg gcgttcttcg
     9121 gcggataagg ggcgtgtttc ctcccagagt gaggcgggaa gagcgatttt gccgatatcg
     9181 tgcaacagcc cggtgagctc cagatcgacg agctcgcgag gtaccagccc catttttcgc
     9241 ccgacggcga ccgaaaggtt gcgcacacgc tcccaatggc cggttcgttc accagcgcgt
     9301 gcctcgagaa tgcgcagact gagtccgagc aggcactcca ttttcacgaa ctcgcccagc
     9361 atggcaaatg tgttctggat ttccggagtg agctcgagat caatgcgtcc ggaggcagcg
     9421 agatctcctg actttgattt gatgctgatc agccactggt tgtgggcgac attgagcgaa
     9481 cgccagtggc ggcccgcata ccgcgcccat tgcggagaag caaggtcggg aggcagttcc
     9541 gtgggtggca gagagccggc aagatcatgg ccacgaaaat ccttgcccca cggaaaccag
     9601 acctgaacct gacagccaac gggttccagg gccttttcca gaatggtgag agccgacttc
     9661 cagacttccg cgacggaact gagttccaga ggtttcatgg cttcctccac aggctgaatg
     9721 gtgtttctcg tcactatacc agaatacgtt catgagcatc atgcggaatt tcacgaagga
     9781 aaagtgcaca gaatgcaact ttgcatatcc tggtcacttt gtatcatttt atatgccgcc
     9841 cagataaagg tctttacata attgccatcc tgggggtatg ctaacaatgc tttattcatg
     9901 cggtttgaat tgccgacatt tcttcgttat cctcccccgg agcatcacac catcagacac
     9961 aaggagcagt tcatgccaaa acatccgact caccctgatc ttacccgccg cctcgcccgt
    10021 atcgccggtc atgtcgaagc cattcgccgc atgctcgatg aagaacgcag ctgccctgaa
    10081 attctgcagc agatgaaagc cgtgctggcc gcgctcgaaa atgcccggcg catcctcgcc
    10141 gaagatcaca tccgccattg tctggtcgat gccattgaac gcaagaacac caaagccgtc
    10201 atcgaagaaa tagaagaggt tttttcacag attctgtgat gtcagatgtc gccaact
//
LOCUS       DAORHD010000012        10045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6011_length_10045_cov_3.525726, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000012 DAORHD010000000
VERSION     DAORHD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_10045_cov_3.525726"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKO06_00635"
     CDS             complement(<1..446)
                     /locus_tag="PKO06_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNV68171.1"
                     /translation="MRSLRSSTILGTGMFLPEKRLTNFDLEKMVDTTDEWITTRSGIK
                     ERRIVEPHQKLSDIAVPAARMALENAGIKPEQINAIILATFTPDRPISATACIIQHKL
                     GCVNAAAMDVEVACSGFVYATSIAHQFIATGSMDYVLVIGADILSKV"
     gene            649..1104
                     /locus_tag="PKO06_00640"
     CDS             649..1104
                     /locus_tag="PKO06_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNV68172.1"
                     /translation="MANQKLRQITRVAAYGLVTRDRQILLCRLSEKLPDCAGKWTLPG
                     GGLAFGEDPATAMVREVVEETGLTVTPGAVAGIDSIVIHGEGTDHHGLRIIYHVQSFS
                     GFLRDESDGTTDTCSWFWFDEAERLPLVELAAIGIRLAREAYQKTSPGK"
     gene            complement(1271..2035)
                     /locus_tag="PKO06_00645"
     CDS             complement(1271..2035)
                     /locus_tag="PKO06_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HNV68173.1"
                     /translation="MDEKIRVGREFLKDAIRLLTDFAATDQSKGVQPPPFEKPVSDGT
                     KRVMLPQPAGLGERFSIDLARAVTNRRSRRAYLPDPLSLEELSWLLWATQGVQKVFGD
                     RSTFRTVPSAGARHALETYLAVSRVTGLEPGLYRFLPIEHELICEYQDKDIGIKLTEG
                     VLGQSFALKAAVSFIWTTIPYRMEWRYGAASHKVIALDAGHVCQNLYLACEAIHCGTC
                     AIAAYHQEKMDALLRVDGTDEFTIYVSPVGRIDGED"
     gene            complement(2072..5122)
                     /locus_tag="PKO06_00650"
     CDS             complement(2072..5122)
                     /locus_tag="PKO06_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68174.1"
                     /translation="MSPANRVTLHPLRVMLFLLLWLLLFLPSRHVWETLRQSDLRLSQ
                     RLQQEARQQNDLVLTLLAQNQRNQNLLVAEQLEQLVLTLTRTAMTLRQESASDALRKA
                     AQTSLTAPNAHLSTVATAALDIRSRVTGMLVQEFRATPGAVVRNQLHTVHAYGLTSPQ
                     QALLEEVFSLNVSEYLGEETATPTHEFVDQISKIFATTGFSRIIAGDLDSSFRGKLHS
                     VNVGGQDCWLFLQLLLDTTAMSPCDEHVPAYPTYIRAYDYTPATKRKLLVGLVAFLLP
                     RSNAPLLSPTDLVRHFMRNGTALALIPETGSPVLADDFHHPELGQYLSRTANVPEFTR
                     DGLILGVGHTWLVQPFRVIAATRPRQPDNWFQIRQHLVRGTLLVILCTLTFLLARSMF
                     FGDWLARRLVQQLSAGFLLASLMPLALAFDVLEQRFQLRTDSLEHQYRQQLRTCLEEF
                     EERIMLTRQTMRQSLEYLARQADLLPVTAVASATPQHSVQSSLARLFQRLLAEAYSSQ
                     VHRVPMNRLVLASLNGCWSVPVEPNDGEKDYLTLFLAAAADLLFQDLGQKPVTSEAVA
                     RNAKQEIILDEFVRLVANTLDPISAFFMLKSANELLILNSGQGNRYIHRLFLPTEASP
                     THQLYAFTGRSQESGAARRILSASLPFPGRVYLTRVPQECFALWPADGMRYPFLRSLF
                     RAMALQPVALSQTIEHEGHTLLIEGWPCKKAQSFLLLGLIDRDALLRQEHLVHLGQLL
                     LILFAISLILFLAFRTAREFLEPVQQLSQGMESLLSGQYSVRIPVTRRDELGQLCLSF
                     NGLIRHLHEGHMLGSMVSQRARRAMQSTSEESAALEGRRVQATIVYVSLHEVDKPVHD
                     ADPETFVRHLSHLISIVCRTAALHGGETDKIMGGKVLLVFGLDAPDEGAGINSALRFA
                     ADLYADRNLPVSPAIGINSGPVISGLLGSGTRRDFTVIGDTVNLAARLCALAGTLPLP
                     QVVLPMSALRFARDLPHTRTLGGTCVKGKQQEVEAVQLLLPSSD"
     gene            complement(5125..8052)
                     /locus_tag="PKO06_00655"
     CDS             complement(5125..8052)
                     /locus_tag="PKO06_00655"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68175.1"
                     /translation="MNSPNRSLLLYILLAILPLFVIDGGWVFLRLQDREGEYLDMEEQ
                     ARQALESMEQNWSLQRQIALHATRLRQAMTSLPDTDDESGSERPQRLLNLAREVFAAP
                     FPTPTVWMYSVDGASPDSPVQLLFARPENQYGKTGPAAFFRFLLRHQQQLSLSESEAK
                     RGQALTEQYLGKGFPGQILARTQRGTPVPVVHARQFHWFVWDYIEVAGRPTTGFILLI
                     PNHPNSQRCAFEVVRREPERDRQAQKGFIRFFRSEIPDILPTSLARSRTFLDWLNDWR
                     REHDIFKLEQRSVPWGQPIGNRVFFFRIIPDCTHFGFISFPRQRFGSPPLFLLLGNGL
                     FLFLGGLVLLRGLMFGTWPHLKLQGRFTGMYLFATALPFTLALVGCLVFLRERENTLM
                     NQLQTRLLRDLRRIDQNKDLSRSRYVSTFHQAVQDPALTQRLKSHGLSDPSVYEVPLS
                     HFHQARPALPLAALYLADWKGNLVGREFKPPEGTQDLTYLIRFYRAGLILLLRNNLQL
                     VGRHDLASSAPPLDDLDNLLVTVHRQGSSVSIANVFQENRLLPDLHQVGSLDLTKLHE
                     LISIDGLYLYSLVIFWRNNDLDLEILREQRRQLLDSDRRLAMMAYRIRDDDLEPLFPE
                     SEAFQKFVRQLGRQAIKRRGSVQGFDAALGWQFVAVPTRNLKNIVLVSGTSLQPIVAE
                     VARLQRGFVLLGIAAFGIILLLGWVTGKHIIEPITRVKHGVEAIVQGDFRARVALDRP
                     DELGQLTQTFDAMITRLEERERLATLVSGKVLDAFSGESDLAAATRARRIEAVILVSD
                     IRNFTSFSEQYSEKTMTALLNHHFDRMNEAIAEFGGRIDKFIGDAIHAIFESEESQSD
                     TPENRSLRAAARMLEYLYHINEERQSQGLFGYRIGIGIAAGEVILGGLGDPEVFFDYT
                     IIGPPLAEAEHLETLSKQVPHFPLVISPRVADRATLTSSFHPLSGYEELARVTDQAFR
                     ADH"
     gene            complement(8065..>10045)
                     /locus_tag="PKO06_00660"
     CDS             complement(8065..>10045)
                     /locus_tag="PKO06_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68176.1"
                     /translation="LMQRLSYQLFAGLLLAALLPLSLAFRLTEEFFQQKFTFLEHQQR
                     RSLLTLFSERERQFRLSVETLKDLLPELVRSSEHSRLLREHVQGITREKPPPYPGVAE
                     VEFSLQKLLRWFYPPPRLQEIFRCHPHLVCAVTWGDISTYFGLRESKSINALPMFFRL
                     TGRAMLNDLNPERFQRVFEASEPLRPKKTAIEEIQQEELQRFGTQIMASIFGEDSILS
                     LFKSPTGVLSMSLGAGTMDVFQIIAPGDAAPDQLYYLFLNTHDIERVAQRRILSRQDG
                     HGPMMYTVRGNRPGFFLSPQTGEQFPFLRPIARKIMYTKVPISSRIEYRGQTYLVEGQ
                     PGSRGDNFIYLGVVPFSESIRREQRVQNILQLSLLLTMFISLLLALNGAVTFLNPVRT
                     LSEALQQVAAGVFTVRLAGDRRDEIGALSLAFNQMTRSLQERHLLGQMVSVSARRAVE
                     SASAEARAAQGYRLHALILYIGIPRFERIRMATDSDALMSGLQRQVTIISRLIQQTGG
                     SIDKIMGEKLLAVFPETETTTSEERSRRAAAILLALSLAERRQELPYPLAVGLQHGQV
                     IAGLLGSGKRRDFTVIGSTVNLAARLEALAETREETRFLTTDRTAMLLKDVVPAEPLA
                     DVTLKGFQEKVTVWNVRPEGISPTPCSGSPLDVVS"
BASE COUNT         2245 a         2693 c         3122 g         1985 t
ORIGIN      
        1 accttcgaga gaatgtccgc gccaatgacc agcacgtagt ccatcgagcc ggtggcaatg
       61 aactgatgcg cgatcgaggt cgcgtagacg aacccggagc aggcgacttc gacatccatc
      121 gcggccgcat tcacgcaccc cagcttgtgc tgaatgatgc acgccgtggc ggaaatgggg
      181 cgatccggag taaaggttgc cagaatgata gcgttgatct gttcgggctt gatgccggcg
      241 ttttccaggg ccatgcgtgc cgcgggaaca gcaatgtctg acagcttctg atgaggctcg
      301 acgatgcggc gttccttgat gccggatcgc gtggtgatcc attcgtccgt cgtgtcgacc
      361 attttttcga ggtcaaaatt ggtcagacgc ttctcgggca ggaacatgcc ggtgccaaga
      421 atggtggatg atcgcaggct tcgcatatct actggaacct ccagtctcac agggagaaaa
      481 gtggcccata gatagcttaa atcagcggta aaagtcaaga ctaaaccggc gacagtatac
      541 ctatttcgca gcgcgctgtg agtctcctgt tgccggaaaa ccccgacacc ggaccggggg
      601 catttcgacc actgttgtcc aggctcgcgc ttgtgctatt ttgctgacat ggcaaaccaa
      661 aaacttcggc agatcaccag agtggccgca tacgggttgg tgacgagaga tcgtcagatt
      721 ctgctgtgcc gactatcgga aaaacttccg gactgtgccg ggaaatggac gctgcccggc
      781 ggcggcctcg cgttcggcga agacccggcg acggccatgg tgcgcgaggt agtggaagaa
      841 accggtttga ccgtgacacc gggagccgtc gccggtatcg attcgatcgt cattcatggc
      901 gaaggcactg atcatcacgg tttgcgcatc atctatcacg tgcagagttt ttccggcttt
      961 ttgcgcgatg aatctgatgg aacgacagat acctgttcct ggttctggtt tgacgaggct
     1021 gaacgtctgc ccttggtcga actggccgcg atcggaattc gtctggcgcg agaagcctac
     1081 cagaaaacgt ctccaggaaa atgaaatcgg acaacggcac gtgtttcccg ctgtgttgaa
     1141 acacccggtt cagagtatct ctggcttctt atcttgatta caaggccagt ccgaaaattc
     1201 catctgggac aaacgaagac cgggaatgcg aatccgactc ggcggcgaaa cgtcgccctc
     1261 acctgaggag tcagtcttcc ccgtcgatgc gcccgaccgg gctcacgtag atggtgaact
     1321 catctgtgcc atcgacgcgc agcagggcgt ccatcttctc ctgatggtag gcggcgattg
     1381 cgcaggtgcc gcagtgaata gcctcgcagg ccagatacag gttctgacac acgtgcccgg
     1441 cgtcgagggc gatgaccttg tgcgatgcgg ctccgtagcg ccattccatg cgatagggaa
     1501 tcgtcgtcca gatgaacgag acggcagcct tgagggcaaa gctctgaccg agcactcctt
     1561 ccgtcagctt gatgccgata tccttgtcct gatactcgca tatgagttca tgttcaatgg
     1621 gaagaaaccg gtacaatcct ggttcgagtc cggtgacccg gcttacggca agatacgtct
     1681 ccagggcatg gcgagctccc gccgatggca ccgtgcgaaa ggtgctgcgg tctccgaaca
     1741 ccttctgtac gccctgtgtc gcccatagta accaggacaa ttcttcgagc gagagtggat
     1801 cgggaagata ggcccgacga ctgcgacggt tcgtcaccgc gcgagccagg tcgatcgaga
     1861 agcgctcgcc caggccggcg ggctgcggaa gcatgactcg ctttgttcca tcagaaaccg
     1921 gtttttcaaa tggaggaggt tgtactcctt tgctctgatc ggtagcggca aaatcagtca
     1981 gcagcctgat ggcatccttg agaaattctc ggccgacgcg aatcttttca tccatactga
     2041 tctcctgtga acgaggtgtt gcggggctga ctcagtctga agacggtaga agaagctgta
     2101 ccgcttccac ttcctgttgc tttcctttca cgcaggtgcc gccgagagtt cgggtatgcg
     2161 gcaggtctcg agcgaaacgg agtgccgaca tcggaaggac aacctgaggc aatggtagag
     2221 ttccggcaag agcacacagg cgtgcggcca gattgacggt gtcgccgatg acggtgaagt
     2281 cgcggcgagt cccggaaccg agcagaccgg agatgaccgg cccgctgttg atgccgatcg
     2341 ccggcgagac cggaaggttg cgatcggcgt aaagatcggc ggcaaaacga agggcggaat
     2401 tgatcccggc gccttcatca ggagcatcga gtccaaagac gagcagcact tttccgccca
     2461 tgatcttgtc ggtttctccg ccgtgaagtg ctgcggtgcg gcagacgatg ctgatcaggt
     2521 ggctcagatg ccggacaaag gtctcgggat cggcatcatg cacaggtttg tcgacctcgt
     2581 gcaacgaaac gtagacgatc gtcgcctgaa cgcgacggcc ttcgagcgcg gcactttctt
     2641 cactggtact ctgcatggcc cggcgggctc gttgcgatac catactgccg agcatatgac
     2701 cttcgtgcag atggcgaatc aagccattga acgaaaggca aagctgtccg agctcatcgc
     2761 ggcgggtgac gggaatccgc acggaatact gtccggagag aaggctttcc atgccctggg
     2821 aaagctgctg tactggttcg agaaactcgc gggcggtgcg gaatgccaga aagaggatga
     2881 ggctgatcgc gaacagaatg agcaacagtt gaccgaggtg aacgagatgc tcctggcgca
     2941 gaagtgcatc acgatcgatg aggccgagga ggaggaaact ctgggccttt ttacacggcc
     3001 aaccttcgat caacagggtg tgtccctcgt gttcgatcgt ttgcgacagg gcgaccggtt
     3061 gcagagccat ggcgcggaac agagaacgca ggaacggata gcgcatcccg tccgccggcc
     3121 agagagcgaa gcattcctgg ggcactcggg ttagatacac ccggccggga aaaggcagcg
     3181 aggcgctcag gattcgccga gcggctccgg actcctgtga ccgtccggta aacgcataca
     3241 gctgatgcgt cggagaggct tcggtgggca gaaaaagtcg gtgaatgtac cggtttccct
     3301 gaccggaatt caagatgaga agctcattag cgctcttcag catgaagaac gctgaaatgg
     3361 ggtcaagcgt gttcgcgacg agtcggacaa actcatcaag aatgatttcc tgcttggcgt
     3421 tgcgggcaac cgcttcactg gttaccggct tttgtccgag atcttgaaac aagagatcgg
     3481 cagccgcggc gagaaacagg gtcagataat ctttctcacc atcgtttggc tcgacgggaa
     3541 cactccagca gccattcaga ctggcaagca caagccgatt catcgggacc cgatgcacct
     3601 gcgaggaata ggcttcagcc agaaggcgct gaaacaatcg cgctaatgac gattgaaccg
     3661 agtgttgagg tgtcgcggat gccactgctg tgactgggag cagatcggct tgtcgtgcca
     3721 gatactcgag agactgacgc atggtttgac gggtcagcat gattcgttct tcgaactctt
     3781 cgagacaggt tcgcagttgc tgacggtatt ggtgctccag cgagtcggta cgtaactgaa
     3841 agcgttgttc gagaacatca aacgccaggg ccagcggcat caacgaggcc agcaggaacc
     3901 cggctgaaag ctgttgaacg agccgtcgcg ccagccagtc accgaaaaac atcgatcgag
     3961 ccagcagaaa ggtgagagtg cagagaatga ccagcagtgt tccgcgcacc aggtgctgtc
     4021 tgatttgaaa ccagttgtcg ggctggcgcg gacgggtggc ggcgatgacg cgaaaaggtt
     4081 ggacgagcca ggtgtgccca accccgagaa tgagaccatc acgggtgaat tccggaacgt
     4141 tcgcggtacg tgagagatat tgtccgagtt cggggtgatg aaaatcgtct gccagaacgg
     4201 gagagccagt ctctggaatc agtgccagcg cggtgccgtt ccgcatgaag tgtctcacca
     4261 gatcggtggg agagagcagg ggagcattcg aacgcggcag cagaaaggcg accaggccga
     4321 ccagcagttt tcgcttggtg gcaggcgtgt aatcataggc acgaatgtag gtgggatagg
     4381 ccgggacgtg ctcatcgcag ggcgacatgg ctgtcgtatc gagcagcagt tgcaggaaca
     4441 accagcaatc ctggccgccg acattgaccg agtgcagttt tccgcggaat gaggaatcca
     4501 ggtctcccgc gatgatacgg ctgaaaccgg tggtggcgaa gatcttgctg atctggtcga
     4561 cgaactcgtg agtcggggtc gcagtctcct ccccgagata ctcggaaaca ttcagcgaaa
     4621 acacttcttc aagcagtgcc tgttgtggcg aagtcagtcc gtaggcatga accgtgtgga
     4681 gttggtttcg caccactgcc ccaggagtgg cgcgaaattc ctgcaccagc attcccgtca
     4741 cgcgagagcg aatatccaga gcggccgtgg cgacggtcga tagatgagca ttcggggccg
     4801 tcaggctggt ttgagccgct ttccgcagag cgtcagaagc gctttcctgg cgaagcgtca
     4861 tggcggtccg ggtgagagtc agaaccagct gttccagctg ctcggctacg agcagattct
     4921 ggttgcgctg attttgggcc agcagagtca ataccagatc gttctgctgg cgcgcttcct
     4981 gctgcagtcg ctgactcagg cggagatcgc tttgtctcaa ggtttcccat acgtggcgtg
     5041 acggcaggaa caagagcagc cagagcagga gaaacagcat gacccgcagg ggatgaagag
     5101 tgacgcggtt ggctggtgac atggtcaatg gtcggctctg aaagcctgat cggtgacgcg
     5161 ggcgagttcc tcgtatccgg acaatgggtg aaagctgctg gtcaacgttg ctcgatcggc
     5221 cacccggggc gagatgacga gcggaaagtg cggcacctgt ttggaaagtg tctcgagatg
     5281 ttccgcttca gccaggggtg ggccgatgat cgtgtagtcg aaaaagacct ctggatctcc
     5341 gagtccgcca agaatcacct cgcctgcggc aatcccgatc ccgatgcgat agccgaacag
     5401 cccttgtgac tggcgctctt cattgatgtg gtacagatac tccaacatgc gtgcagcggc
     5461 gcgcaaagaa cggttttccg gagtgtccga ctgggactct tcggattcga aaatcgcatg
     5521 aatcgcgtcg ccgataaact tgtcgatgcg accgccgaat tcggcgatgg cttcgttcat
     5581 gcgatcaaag tgatgattca acagggcggt catcgtcttt tctgaatact gttcggagaa
     5641 cgaagtaaag ttgcgaatgt ctgaaaccag gataaccgct tcgatccgac gggctcgggt
     5701 cgccgcggcc aggtctgact ctcccgaaaa ggcatcgaga acttttcccg aaaccagcgt
     5761 ggccagacgc tcgcgttctt cgaggcgagt gatcatcgcg tcgaaagtct gcgtcaattg
     5821 tccgagttcg tcggggcgat cgagagcaac acgagcccga aagtcgccct gtacaattgc
     5881 ctcaactccg tgtttcacgc gtgtgatcgg ctcaatgatg tgcttgccgg taacccagcc
     5941 cagaagcaga atgatgccga aggcggcgat gccaaggagc acgaagccgc gttgcaggcg
     6001 ggcaacttcg gcgacgatcg gctgcagtga ggtgccactg accaggacga tattcttgag
     6061 gttgcgggtg ggcacggcaa caaactgcca gcccagcgcg gcatcaaatc cctggactga
     6121 gcctcgtcgt ttgattgctt gtcgcccgag ttgccggacg aacttctgaa atgcctcgct
     6181 ctcgggaaac aacggctcaa gatcgtcgtc tcggatccga taagccatca tcgcgagacg
     6241 ccggtcagag tcgagcagct gccggcgctg ctcacgcagg atctcgagat cgagatcatt
     6301 gttccgccag aagatgacca gtgagtacag atacaggccg tcgatcgaaa tgagctcatg
     6361 caatttggtc agatcgagac tgccgacctg gtgtaaatcc gggaggagac ggttttcctg
     6421 aaacacgttg gcgatcgaaa cactcgaccc ctgacggtgg accgtgacca gcagattgtc
     6481 gagatcatcc agcggagggg cgctggatgc aagatcatgg cggcccacca actgaagatt
     6541 gttgcgcaag agcagaatca atcccgcgcg ataaaatcgt atgaggtagg tgaggtcctg
     6601 agtgccttcg gggggcttga attcccgacc gaccagattc cctttccagt cggcgagata
     6661 caacgcggcg agtggcagag ccggtcgggc ctgatgaaaa tgggacagag gaacctcata
     6721 cacgcttgga tccgatagcc catggctttt cagccgttgc gtcagggcgg ggtcctgtac
     6781 tgcctgatga aaagtcgaga catagcggct gcgggagaga tccttgttct ggtcgatgcg
     6841 tcgcaggtca cgtagcagcc gtgtctgcag ctgattcatc agggtgttct cgcgctcccg
     6901 aagaaaaacg agacaaccga ccagcgccag ggtgaaggga agggcagtgg cgaacaagta
     6961 catgccggtg aaccgaccct gaagtttcag gtgcggccat gttccgaaca tcaggccgcg
     7021 caacagtacc aggccgccga gaaacagaaa aagaccattg ccaagcagca gaaacagggg
     7081 aggggagccg aaccgctgcc gtggaaaaga gatgaatccg aagtgcgtgc aatccggaat
     7141 gatgcggaag aagaacaccc ggtttccgat tggctgtccc cacggcaccg atcgctgctc
     7201 gagtttgaag atgtcgtgct cgcgacgcca gtcgttcagc cagtcgagaa aggtgcgaga
     7261 gcgggcgagg gaggtcggca ggatgtcggg aatctcactg cggaaaaagc ggatgaaccc
     7321 cttctgagcc tgacgatccc gttccggttc gcgtctgacg acctcgaagg cgcatcgctg
     7381 tgaattcggg tggttcggga tcagcaggat aaagccggtc gttggtcgac cggcgacctc
     7441 gatgtaatcc cagacgaacc agtggaactg gcgcgcatga acgactggga caggagtgcc
     7501 gcgctgcgtg cgcgcgagaa tctgaccagg aaatcccttg ccgagatact gctcggtgag
     7561 cgcctgccct cgttttgcct cggattcaga cagtgacaat tgctgctggt ggcgcaacag
     7621 aaagcggaag aaggcggcag gaccggtttt tccgtactgg ttctccggcc gtgcgaacag
     7681 caattgaacc ggactgtcgg gactggcgcc gtcaacggaa tacatccaga cggtcggcgt
     7741 tgggaacggc gcggcgaaga cttcgcgtgc cagattcagg agccgctgtg gacgctcaga
     7801 gccggattca tcatccgtat cggggagcga tgtcattgcc tgccgcagtc tggttgcgtg
     7861 cagggcgatc tgcctctgca gcgaccagtt ctgttccatg gattcgagcg cctggcgtgc
     7921 ctgttcttcc atgtcgagat actcgccctc tcgatcctgc aggcgcaaaa acacccagcc
     7981 gccatcgatc acgaacagcg gcagaatggc caggagaata tacagcagga gagagcggtt
     8041 gggggaattc atctcacgac ggcctcatga taccacgtcg agcggcgacc cgctgcatgg
     8101 ggtgggggaa ataccttcgg ggcgcacgtt ccacaccgtg actttctcct gaaatccttt
     8161 caacgtcacg tcggcgagtg gctcggcggg cacgacgtct ttcagcagca tcgcggtgcg
     8221 atcggtcgtg agaaatcttg tctcttcccg agtttcggca agtgcttcga ggcgcgccgc
     8281 cagattgacc gttgacccga tgaccgtgaa atcgcgccgt tttcccgacc cgagaagacc
     8341 ggcgatgacc tgtccgtgct gcaggccgac ggcaagaggg tagggaagtt cctgacgccg
     8401 ttccgccagc gagagagcca gcaggatggc agccgcccgc cgcgatcgct cttctgacgt
     8461 tgtcgtctcg gtttcaggga acaccgccag cagcttttca cccatgattt tgtcaatcga
     8521 tcccccggtt tgctgaatca aacgtgagat tatcgtgact tgccgctgca ggcccgacat
     8581 cagtgcgtcc gagtcggtcg ccatacgaat gcgctcaaat cgaggaatgc cgatatagag
     8641 aatgagtgcg tgcagacgat atccctgtgc cgctctcgct tcggccgaag ccgattcgac
     8701 ggcgcgtcgg gccgaaaccg agaccatctg tccgaggaga tgccgttcct gcagggaacg
     8761 ggtcatctgg ttgaaggcca gagacagcgc tccaatctcg tcacgacggt ctccagccag
     8821 ccggaccgtg aagactccag ccgcgacctg ctgcagcgct tcagagagtg tgcgtaccgg
     8881 gttcaggaag gtgactgccc cattgagagc gagaagcagg gagataaaca tcgtcagcag
     8941 taacgacagt tgcagaatat tctgtacacg ttgttcgcgc ctgatgcttt ccgagaaagg
     9001 aacgactccg aggtagatga agttgtctcc tcgcgaaccg ggctggccct cgacaagata
     9061 ggtctggccg cgatactcga tgcgcgaact gatcggaacc ttggtgtaca tgatcttgcg
     9121 ggcgatgggc cgcaagaaag gaaactgttc gccggtctgt ggcgagagaa agaaaccggg
     9181 acgatttcca cgaacggtgt acatcatcgg tccatggccg tcctgccggc tgagaatgcg
     9241 gcgttgcgcc acgcgctcga tgtcgtgcgt attgaggaac agataataga gctggtctgg
     9301 cgcggcatct ccgggcgcga tgatttggaa gacgtccatg gtaccggcac cgagagacat
     9361 actgaggacg ccggtcggtg acttgaacaa gctcagaatg gaatcttccc cgaagatcga
     9421 ggccatgatc tgcgtgccga atcgttgcaa ttcttcctgc tgaatttctt caatagcggt
     9481 cttcttcggt cgcagtggct cggacgcttc aaaaacacgc tgaaatcgct cggggttgag
     9541 atcgttcagc atcgctcgtc cggtgaggcg aaaaaacatc ggcagggcgt tgatggattt
     9601 cgattctcgc aggccgaaat acgtactgat gtcaccccag gtcacggcac agaccagatg
     9661 cggatgacag cgaaagatct cctgcaggcg cggcggcggg taaaaccagc ggagaagttt
     9721 ctgcaggctg aattcgacct cagccacacc gggatacggt gggggttttt ccctggtgat
     9781 accctggaca tgttcgcgca ggagtcgtga atgttctgac gaacggacaa gctctggcag
     9841 caggtctttc agcgtctcga cactcagacg gaactggcgt tccctttctg agaacagggt
     9901 gagcagcgag cggcgttgct ggtgctcaag aaaggtgaat ttctgttgga agaactcctc
     9961 ggtcaagcga aatgccagag acaacggcag cagagcggcc agcagcaacc cggcaaacag
    10021 ttgatacgac aggcgctgca tcagc
//
LOCUS       DAORHD010000013         9820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6243_length_9820_cov_4.188735, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000013 DAORHD010000000
VERSION     DAORHD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6243_length_9820_cov_4.188735"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..791)
                     /locus_tag="PKO06_00665"
     CDS             complement(45..791)
                     /locus_tag="PKO06_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV68177.1"
                     /translation="MTLPTRSTLLPADLFRRLALLFTLFGLIPLISHRGNAATNFKPA
                     KLRPLQQAHLDPTLQQFWKQMALATRTRNFAFLEPHIDPNLRWTLGPESGIKSFMRYW
                     NLDKNPQQSIFWKELDRVLHLGGAFINNDRNRFQAPYVSACWPDEYDGFTHAAIIADN
                     VPLRQTPEPAGKVIGWLSYDIVRLDIDQLSHAPRQKLQGETHPWVQVTTAQGHTGFVW
                     GKYVRSPIDIRLMVEKIDGIWKITCFLAGD"
     gene            complement(788..1348)
                     /locus_tag="PKO06_00670"
     CDS             complement(788..1348)
                     /locus_tag="PKO06_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009119481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HNV68178.1"
                     /translation="MVPISASGVRLLSETERERFVQAALDQVGVTVMYDPAYVKLPYP
                     GGDVPMERGVCTDVVIRALRKLGKDLQVLVHEDMRRHFSAYPKKWGLKKPDRNIDHRR
                     VPNLMCWFTREGYSQPITSRETDYLPGDIVAWALSDGRPHIGIVSHLPVPETRRLLCV
                     HNIGAGAQLEDVLFAFQIIGHYRMFK"
     gene            complement(1413..3821)
                     /locus_tag="PKO06_00675"
     CDS             complement(1413..3821)
                     /locus_tag="PKO06_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV68179.1"
                     /translation="MSSEKKSEFTADGDVSSTRSCGSNHPPSAPADDTATRASPSGSS
                     GETVYRSLSEHLPGIVYRLYLREQNRMEFFNDMVRQITGYAAEELQKSRVLSIDPLIH
                     QEDLDRVITSVSASLPSMNPYQIEYRIIHRDGSIRHLEERGRPIPGGDGFPLYVDGVI
                     FDVTEKKNLEQMRKRYEFIVNTSREFMTMVRRDFVYEAVNEAYCQAHKKSREQIIGHT
                     VTDVWGPEAFSRIRPYFLQCFEGKEVHYRFSFPFAALGMRYFDVTYYPYYGDDREVTH
                     VIVVSRDMTRSHQMEVERDRLAIAIQQAAEGVLITDVEGTIEYVNPAFERISGYTSTE
                     LIGKNPRLFKSGNQPPATYEELWRTVKNGNVWKGHFINAKQDGSLYEAHSTISPVRDE
                     SGRIMHFVAVMNDITKEVALERQMRQSQKLEAIGTLAGGIAHDFNNILGAILGFADLT
                     RVELDESLPVQDNLREIIQASRRAQELISQILTFSRRVEPRRIPLLLHQAAQEGMSLL
                     RATVPKTVLIHNDFACQQTGMMGDPVQMHQILINLVTNAIQALPDGRGSVYVSVQPHL
                     VTPDTIESDPLHLPAGKYVLLEVRDSGVGMDKEVLDRIFEPYFTTKPVGQGNGLGLAM
                     VHGIVKEHDGHISVHSSPGHGTSFRIYFPACDVQPDSSPAREPVLSTSRGRILLVDDE
                     PALMSFGRKALQHLGFEVEATNDSRAALTLLQEEPIRFDAIITDIAMPGLSGPDLIEE
                     LRKLNQDIPVFMMSGFGSGQISPENLARLKIKLLLKKPFSISDLQQALHCITPSASSN
                     RRHL"
     gene            3903..4163
                     /locus_tag="PKO06_00680"
     CDS             3903..4163
                     /locus_tag="PKO06_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV68180.1"
                     /translation="MKRQQGRPTGLTRGGKEWTPRFLERLDAERCTGCGFCVKVCPVH
                     VFQGPPHARPTVVRLEECIGCRVCFNLCKDRAIELEELEGET"
     gene            4160..4927
                     /locus_tag="PKO06_00685"
     CDS             4160..4927
                     /locus_tag="PKO06_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV68181.1"
                     /translation="MSACIGLDLGSRRSKAVVLEQGQLREKAVFDAWVLAHEQIGDWV
                     RQRQPAMVGATGYSRQQARRVFEARVQTEIAAFAAGAGLLAPGALTLFDIGGQDAKVI
                     RLDGAGVALDFDMNDKCAAGTGKFFELLAGTLGVTYADMLSMAGNATEAATISSTCAV
                     FAESEIIGRLADGTDRSHLARGAMRSVAERLAAMSNRLGCVSPVILVGGGANPALADE
                     LSQLLRTEILLPTDGAFFGAVGIARLVSGELPSRGTS"
     gene            4924..6441
                     /gene="hutH"
                     /locus_tag="PKO06_00690"
     CDS             4924..6441
                     /gene="hutH"
                     /locus_tag="PKO06_00690"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNV68182.1"
                     /translation="MIELNGSRLDLNSLRRIADGEPVAIAPDVVDRVQASRRLVERLV
                     SEERVVYGVTTGFGHLCNTRISRDDLERLQTNLIRSHATGVGTPFSVPEVRAMIAIRI
                     NSLLHGVSGVRVEVVQGLADLLNHHVTPCVPEKGSVGSSGDLAPLSHIMLVLMGEGEV
                     LGEDGRPAPAAPALAKAGLKPLQLLAKEGLALINGTSVMTGVLGLAVEKGLKIAQSAD
                     IIAALTLEVLRGSDAPFDEAFIALRPYEGLKHVAHNIRSCLVDSEVRSSHLHCTKVQD
                     AYSLRCIPQVHGASREALRHLAEQVQIEFNSVTDNPILFDEHKIISGGHFHGQPMALV
                     ADYFGIAMAELGNISERRIDRLLNPLVSGMAPFLAENPGVNSGLMIVQYTAASLVSEN
                     KVLAHPASVDSIPTSAYQEDHVSMGTIAARKGAKILEHSAQVLAIEWLCAAQAYEFVK
                     PLKLGKGTGLAYELLRQTLPPLKEDRIMYTDLAAAQQLLWKGELVDHVAKEIPIR"
     gene            6413..8092
                     /gene="hutU"
                     /locus_tag="PKO06_00695"
     CDS             6413..8092
                     /gene="hutU"
                     /locus_tag="PKO06_00695"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNV68183.1"
                     /translation="MSPKKSPSASRVVHPPTGTKLHCKGWLQEAAFRMAQHNLDPEVA
                     EKPEELIVYGGIGKAARNWECYDAILKTLQRLENDETLLVQSGKPVAVFKSHPDAPRV
                     ILANSLLVPRWATWEHFHELDRKGLMMYGQMTAGSWIYIGTQGILQGTYETFAAIAQK
                     HFGGSLAGTISLTGGLGGMGGAQPLAVTMNDGVCITVEVDRTRIQRRIDHRYCDVMVE
                     SIDEAIKRAQEAKKQKKPLSIGLLGNCAEVLPEMFKRGFRPDILTDQTSAHDPLNGYV
                     PAGMSFEAALELRRKDPNGYVEKAKDSIVAHVKAMLEFMDAGVITFDYGNNIRGQALD
                     RGLKRAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDPQDIYTTDEAMVKLFPDHAPLK
                     RWLKKAREQVHFQGLPSRICWLGYGEREKAGLMFNELVRTGKVKAPIVIGRDHLDAGS
                     VASPYRETEAMKDGSDTVADWPILNALINAVNGATWVSVHHGGGVGMGNSIHAGMVIV
                     ADGTPEAERRLRRVLTSDPGMGVVRHVDAGYDLAIDCARKHGVDIPMMSKK"
     gene            8125..9042
                     /gene="ftcD"
                     /locus_tag="PKO06_00700"
     CDS             8125..9042
                     /gene="ftcD"
                     /locus_tag="PKO06_00700"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNV68184.1"
                     /translation="MEIIESIINISEGRDAETVNYIVESVRQTPECFLLDVSSDADHN
                     RTVITFIGNRASLTKAIKELYKRSLEKIDLRKHKGEHPRMGAVDVVPFVPIRDITLED
                     TIAFSKEVGKMIWETYKVPVYFYEETQPVADRKNLAAIRKGEFEGLPDKMKKPEWKPD
                     LGECAPHESAGASVVGCRMPLIAFNVNLGTANLDIANAIARHVRGSSGGLVCVKAMGV
                     ELKQRGIVQVSMNMVNYKKSPLFRVFEMIKAEADRYGVPVVGSEIVGLIPQDAMFEVA
                     EFYLRVEGYTPACILENKINTVLFDQLKK"
     gene            complement(9113..>9820)
                     /locus_tag="PKO06_00705"
     CDS             complement(9113..>9820)
                     /locus_tag="PKO06_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68185.1"
                     /translation="ALHELSHQFFTTRTIGSHIRDHLKTNDDATGFLTGVYLNEGLAT
                     ALGNGLYSRAIGSDDPQWYARDTINRYGHALLPLARQWIDRHRAIDREFFLAAHAAFQ
                     KLFPRYEQRWEFAFSRIAWMGSAEEAVFFTEGLKKHLPSYRLVEPSDDGRPLARVFKI
                     SSGRDLTKLPLSDRLRSRALVFSHSCENFILSDFDRDRDQFEIVFCSSKPEKFAALLE
                     RAFQLVYRSRFVTLSNH"
BASE COUNT         2125 a         2615 c         2931 g         2149 t
ORIGIN      
        1 tcttgatccg gcaaaagtcc gggttctgca ggctgctgat ctcttcaatc accggccaga
       61 aaacaggtga ttttccagat tccgtcaatt ttttccacca tcaggcggat atcgatcggg
      121 ctgcgaacat acttgcccca gacaaaaccg gtgtgtcctt gagccgtggt cacctgcacc
      181 cagggatgtg tttcgccctg caacttctgg cgtggggcgt gtgacaactg gtcaatatcg
      241 agtcggacga tgtcgtacga gagccaaccg atgactttgc ctgctggttc gggagtctgg
      301 cgcagcggca cgttatcggc aatgatggcc gcgtgcgtga aaccatcata ttcgtccggc
      361 cagcacgccg acacatacgg ggcttgaaat cggttgcgat cattgttgat gaacgctcca
      421 cccaggtgca gtactcggtc gagttctttc cagaaaatcg actgctgagg gtttttgtcc
      481 aggttccagt agcgcatgaa gcttttgatg cctgattccg ggccgagggt ccagcgaaga
      541 ttcgggtcga tgtgaggttc cagaaaagca aagttgcgcg tgcgtgttgc cagcgccatt
      601 tgtttccaga actgctgcag cgtcgggtca agatgcgcct gctgcaacgg gcgcaacttc
      661 gccggtttga agttcgtcgc cgcgttgccg cggtgggaaa tcagcgggat caacccaaac
      721 aaggtgaaaa gcagcgccag gcgcctgaag agatcagccg ggagcaacgt ggatctggtg
      781 ggcagagtca tttgaacatc cgatagtgac cgatgatctg aaaagcgaac aacacgtctt
      841 caagctgagc gccggcacca atattatgta cgcagagcag acgtctggtt tctggaactg
      901 gcagatgact gacgatgccg atatgcggcc ggccgtcgct caaggcccag gcgacgatat
      961 cgcccggaag atagtccgtc tcacgtgagg tgatcggttg ggaatatcct tcacgcgtga
     1021 accagcacat gagattcgga acccgccggt gatcaatgtt tcgatcgggc tttttcagac
     1081 cccatttttt cgggtacgcg gaaaagtggc ggcgcatatc ttcatgtacc agcacctgaa
     1141 gatcctttcc gagttttcgc agggcgcgaa tcaccacgtc ggtgcagact ccccgttcca
     1201 ttggcacatc accacccggg tagggcagtt tcacgtatgc tggatcatac atgaccgtga
     1261 cgccgacctg atcgagggct gcctgaacga accgctcgcg ctccgtctcg ctgaggaggc
     1321 gcacaccgct tgcggagatc ggaaccatga tgcagatcat catgaacagc aggagaaacg
     1381 aacgaagact gcgggttgtg atcaacggca tatcagaggt gtctccgatt cgatgaggca
     1441 ctgggggtga tacagtgaag agcctgttgg agatcgctga tgctgaaagg cttcttcaga
     1501 agcaacttga tcttgagtcg ggcgaggttt tccggcgaaa tctgtccgct tccgaaccct
     1561 gacatcatga aaactggaat gtcctggttg agttttctca attcttcgat cagatcaggt
     1621 ccagagagtc ctggcatggc aatgtccgtg atgatcgcgt caaatcggat aggttcttcc
     1681 tgtaagagag taagagcggc tctggagtcg ttggtggctt caacctcgaa gccaagatgc
     1741 tgcaaggctt tgcggccgaa actcatcaag gccggctcat catcgaccag gagaattctt
     1801 cctcgactcg tggagagaac cggttcacgt gccggcgacg aatcaggctg aacgtcacaa
     1861 gcgggaaaat agatgcgaaa gctcgttcca tgtccgggac tgctgtggac ggagatgtga
     1921 ccatcatgct ccttgacgat tccatgaacc atggcgagac ccaggccatt gccctgaccg
     1981 accggcttgg tggtgaagta cggctcaaag atgcgatcaa gcacttcttt gtccatcccc
     2041 acaccggaat cgcgaacttc gagaagcaca tacttgccgg cgggaagatg caggggatcg
     2101 ctctcaatgg tgtctggcgt taccagatgt ggctgaacgc tcacatacac cgaaccccga
     2161 ccgtctggaa gagcctgaat ggcattggtc acgaggttga tcaggatctg atgcatctgc
     2221 acgggatcac ccatcattcc cgtctgttga caggcaaaat cattgtggat gagaacggtc
     2281 ttgggaacgg tggcgcgcag caagctcatc ccctcttgag cggcctgatg caacagcaat
     2341 gggatccgcc gtggttcgac tcgtcgactg aacgtgagaa tctgggagat cagttcctgg
     2401 gcccgccgac tggcttggat gatttctcgc aggttatcct ggaccggaag agactcatca
     2461 agctcgactc tggtcaggtc agcaaatccg agaatcgcgc cgagaatatt gttgaaatca
     2521 tgcgcaatac cgcctgcgag ggtgccaatg gcttcgagtt tttgcgattg ccgcatctgc
     2581 cgctcgagag ccacctcctt ggtgatatca ttcatcaccg ccacgaagtg catgattcgg
     2641 ccggattcat ctcgtaccgg ggagatggtc gagtgtgctt cgtacaagct accgtcttgt
     2701 ttagcgttga tgaaatggcc tttccagacg tttccgtttt taacggtgcg ccagagttcc
     2761 tcgtacgttg caggcggctg gttcccgctc ttgaataatc gcgggttttt tccgatcagt
     2821 tcagtggatg tgtacccgga gatgcgttcg aaggcgggat tcacatactc gatggttcct
     2881 tcgacgtcgg tgatgagaac cccctcagcg gcttgctgta tggcaatggc cagacgatca
     2941 cgctcgactt ccatctgatg cgacctggtc atgtcacgtg aaacgacgat gacatgcgtg
     3001 acctcacggt cgtcgccata gtaggggtag taggtgacat cgaaatagcg catgccgaga
     3061 gcggcaaacg gaaatgaaaa ccggtagtga acttcttttc cttcgaaaca ctgaagaaag
     3121 tagggtcgaa tacgcgagaa ggcttcgggc ccccagacat cggtcacggt gtgaccgatg
     3181 atctgctcgc gcgacttttt gtgagcctga cagtaggctt cgtttaccgc ttcgtagaca
     3241 aagtcccgtc tgaccatcgt catgaattcg cgtgaggtat tgacgatgaa ctcgtagcgt
     3301 ttacgcattt gctcgaggtt cttcttttcc gtcacgtcga agatgactcc gtcgacatac
     3361 aacgggaacc cgtcgcctcc tggaatcggt ctgcctcgct cttccagatg ccggatactt
     3421 ccgtcacgat ggataatgcg gtattcgatc tgataggggt tcatcgaggg gaggcttgcg
     3481 ctgaccgatg tgatgacacg gtccagatct tcttgatgaa tcaaagggtc gatcgagagc
     3541 acacggctct tctgaagctc ctcggcagca tatccggtga tctgtcgcac catgtcgttg
     3601 aaaaattcca tgcggttctg ttcgcgcaga tacagtcgat acacgattcc gggaagatgt
     3661 tcggaaagag agcgatagac tgtttcgcca gatgatcccg aaggtgacgc ccgggttgcc
     3721 gtatcgtccg caggagcaga tggtgggtga ttgctgccgc acgatcgggt cgaagaaacg
     3781 tccccgtcgg ccgtgaattc gctcttcttc tctgacgaca tggttcgtga cactccttga
     3841 ctgattatat ggaggttctg tgaaagtgcc aagtctcgag atgaagtggt ataattcggc
     3901 ccatgaagag acagcaaggg cgccccacag ggctgacccg gggtggaaag gaatggacgc
     3961 ctcgttttct cgagcgcctc gatgctgagc gttgcaccgg gtgcggtttc tgcgtgaaag
     4021 tctgtccggt ccatgtcttt cagggacccc ctcacgctcg cccgaccgtt gttcggctcg
     4081 aagagtgcat tggctgccgt gtctgtttca atctctgtaa agaccgtgcc atcgaactgg
     4141 aagagctgga gggagagacg tgagtgcctg catcggtcta gacctcggat cacgccggtc
     4201 gaaggcggtc gttctcgaac agggtcagct gcgtgaaaag gctgtcttcg atgcctgggt
     4261 actggctcat gagcagattg gcgactgggt gcgtcagaga cagccggcga tggttggcgc
     4321 gaccggttac agccgccagc aggctcgccg cgttttcgag gcgcgggtgc aaaccgagat
     4381 tgcggcgttt gccgccgggg ccgggttgct cgcgcccggg gcactcaccc tctttgatat
     4441 cggaggccag gatgcgaagg tcattcgcct cgatggtgcc ggtgtcgctc tcgattttga
     4501 catgaatgac aagtgcgcgg ccggtaccgg caagtttttc gaattgctgg ctgggactct
     4561 tggcgtcacc tatgccgaca tgttgagcat ggcggggaac gcaaccgagg ccgcgactat
     4621 atcgagcacc tgtgctgtgt ttgccgaatc agagatcatt gggcgtctgg ctgacggcac
     4681 ggaccggtcg catctcgcgc gcggcgccat gcgatcggtg gctgaacggt tggcggccat
     4741 gagcaatcgg ctggggtgcg tttctcccgt gattctggtg ggcggtggtg cgaaccccgc
     4801 gctggctgac gaattgtccc agttgctgcg cacggaaata ctgcttccaa ccgacggtgc
     4861 gtttttcggt gccgttggta ttgcccgact ggtgtcgggc gaacttccct ctcgaggaac
     4921 atcatgatcg aactgaatgg cagtcggctg gatctgaaca gccttcgtcg cattgccgat
     4981 ggtgaacccg tcgcgattgc tccggatgtc gtcgatcgag tccaggcttc gcgtcgtctg
     5041 gtcgagcgtc tggtcagcga agaacgcgtg gtctatggcg tcaccaccgg attcggacat
     5101 ctgtgcaaca cacgaatcag tcgcgatgat cttgaacgac tgcagaccaa tctgattcgc
     5161 agtcacgcga ccggcgtcgg caccccgttt tcggtgcccg aagtgcgagc catgatcgca
     5221 attcgcatca attcgctgtt acatggggtc agcggcgtgc gtgtcgaagt cgtgcagggt
     5281 ctggccgatc tgttgaatca tcacgtgaca ccgtgtgtcc ccgagaaagg ctcggtcggc
     5341 agctcaggag atctggctcc gctttcgcac atcatgctgg tgttgatggg cgaaggcgaa
     5401 gttctcggcg aagacggtcg tccggcgccg gctgcgcccg ctctcgcgaa ggctggtctc
     5461 aagccgctgc agttgctggc caaggaagga ctggcgctga tcaatggcac gtcggtcatg
     5521 accggcgtgt tgggtctggc ggtcgaaaag ggactgaaaa tcgctcagag cgcggatatc
     5581 attgccgccc tgacgctcga agtgctgcgt ggatctgatg ccccatttga tgaagcgttc
     5641 attgcgttgc gcccctatga gggtctgaaa cacgttgccc acaacattcg ctcgtgcctg
     5701 gtcgacagcg aggtgcggtc gagtcatctg cactgcacga aggtgcagga tgcctacagc
     5761 ctgcgttgta ttccccaggt tcacggagcc tctcgcgaag cgcttcgaca tcttgccgaa
     5821 caggtacaga tcgaattcaa ttcagtcacc gacaacccga tcctgttcga cgaacacaag
     5881 atcatcagcg gcgggcactt tcatggacag cccatggccc tggtcgccga ctatttcggc
     5941 attgccatgg ccgaactcgg caacatcagc gaacgccgca tcgaccgtct gctgaatccg
     6001 ctggtcagcg gcatggcacc atttctggcc gaaaacccgg gggtcaactc gggtttgatg
     6061 atcgtgcaat acacggctgc ttcgctggtc agcgaaaaca aggtcctggc tcacccggcg
     6121 tcggtcgatt cgattccgac ctcagcgtac caggaagatc atgtcagtat gggcacgatt
     6181 gccgcacgca agggtgccaa aattctcgaa cactcggcgc aggtactggc catcgagtgg
     6241 ctctgtgccg cgcaggccta tgagttcgtc aaaccgttaa aactgggaaa aggaaccggg
     6301 ctggcgtacg agttgctgcg gcagacgtta ccgccgctga aggaagatcg gatcatgtat
     6361 accgacctcg ccgccgccca acaactgctc tggaaaggag aactggtcga tcatgtcgcc
     6421 aaagaaatcc ccatccgcta gtcgtgtcgt tcatccaccg accggcacca aactccattg
     6481 taagggttgg ttgcaggaag ctgcgtttcg catggcgcag cacaacctcg accccgaggt
     6541 agccgagaag cccgaagaac ttatcgtcta cgggggaatc gggaaagccg cgcgtaactg
     6601 ggaatgctac gatgccattc tgaaaaccct tcagagactc gagaatgatg agactctgct
     6661 agtacagtcg ggcaaaccgg tcgcggtgtt caaatcgcat ccggatgccc cgcgggttat
     6721 tctggcaaac tcgctgctgg tccctcgctg ggcgacctgg gaacattttc acgaacttga
     6781 tcgcaaaggt ctgatgatgt acggccagat gacggccggt tcctggatct acatcggcac
     6841 tcagggtatt ctgcagggaa catatgaaac cttcgccgcc atcgctcaga agcactttgg
     6901 aggttctctg gctggtacga tctctctgac cggtggtctg ggcggaatgg gcggcgctca
     6961 gcctctcgcg gtgaccatga atgacggtgt ctgtatcacc gtcgaagtgg accgcacccg
     7021 tattcagcgt cgtatcgatc atcggtattg tgatgtcatg gtcgagtcga ttgatgaggc
     7081 catcaagcga gcgcaagaag ccaaaaagca gaagaagccg ctttcgatcg gcctgctggg
     7141 caactgcgcc gaagtacttc ccgaaatgtt caaacggggt ttccgcccgg acattctgac
     7201 cgatcagacc tccgcgcacg atccgctgaa cggatatgta ccggcgggga tgtcgtttga
     7261 agctgctctc gagctgcgtc ggaaagatcc gaacggatac gtcgagaaag ccaaggattc
     7321 gatcgtcgcc cacgtcaagg cgatgctcga gttcatggat gcgggagtca tcacgtttga
     7381 ttacggcaat aacattcggg gacaggcgct tgatcggggt ctgaagcggg cattcgattt
     7441 tcccggattc gtgccggcat atattcgccc cctgttctgc gaaggcaagg ggccgttccg
     7501 ctgggtggcg ctgtccggag acccgcagga tatctatacg accgacgaag ccatggtgaa
     7561 gctgttcccg gaccacgcgc cgctgaaacg atggctgaag aaggctcgcg agcaggtgca
     7621 tttccagggt ctgccgtcgc gcatctgctg gcttgggtat ggcgagcgtg agaaagcggg
     7681 gctgatgttc aacgaactgg tcagaacggg caaggtgaaa gcccccatcg tcatcggtcg
     7741 agatcatctc gacgccggat cggtcgcctc accatatcgt gaaaccgagg ccatgaaaga
     7801 tggttcagac accgtggccg actggccgat cctgaatgcg ctgatcaatg cggtaaacgg
     7861 agcgacctgg gtcagcgtgc atcacggtgg cggtgttggc atgggaaact cgattcatgc
     7921 cggcatggtt atcgtggctg atggaacgcc cgaggccgag cgtcgtctgc gccgggtgct
     7981 gacctctgac ccgggcatgg gtgtcgtccg tcatgtcgat gccggatacg atctggcgat
     8041 cgattgcgcg cgcaagcatg gggtcgatat tcccatgatg tcgaaaaagt aacgcgattc
     8101 gcgaataccc cacaggagaa aaccatggaa atcattgaaa gcattatcaa catcagcgag
     8161 ggccgcgatg ccgagacggt gaactacatc gttgagtcgg tgcgccagac tcccgagtgt
     8221 tttctgctcg atgtgtcatc ggacgccgat cacaatcgta cggtcatcac cttcattggc
     8281 aatcgtgcca gcctgacgaa ggcgatcaag gagttgtaca aacgcagcct cgaaaagatt
     8341 gacctgcgca agcacaaagg ggaacatccc cgcatgggtg cggtggacgt ggttccattc
     8401 gttcccattc gcgacattac gctcgaagac accattgctt tcagtaaaga agtcggaaag
     8461 atgatctggg aaacctacaa agtaccggtg tacttctacg aagagacgca gccggtagcc
     8521 gaccgcaaga atctggcggc gattcgcaag ggcgagtttg aggggttgcc cgacaagatg
     8581 aagaagcctg aatggaagcc ggatctcggt gaatgtgcgc cgcatgagag cgccggagca
     8641 tcggtcgtcg ggtgccgcat gccgttgatt gccttcaacg tgaacctggg cacggcgaat
     8701 ctggatattg ccaacgcgat cgctcgccac gttcgtggca gttcgggggg gctggtctgt
     8761 gtcaaggcga tgggcgtgga actcaaacag cgcggtattg ttcaggtatc gatgaacatg
     8821 gtgaactata agaagtcacc gctgtttcgc gtattcgaga tgatcaaggc ggaagcggac
     8881 cgctatggag tcccagtcgt tggatcagaa atcgttggtc tgataccgca agacgctatg
     8941 ttcgaagtgg cagaattcta cctgcgagtc gagggctaca ctccggcttg tattctcgaa
     9001 aacaagatca acaccgtatt gttcgaccag ctcaagaagt agactgacgg aacagagttc
     9061 gaaaaccacc ggccggcgcg aatctcgcgc cggccgatgg tgcgttcgag tgtcagtggt
     9121 tcgaaagagt cacgaaccgc gatcgataca caagctgaaa ggctcgttcg agcaacgccg
     9181 cgaatttctc tggtttcgag ctgcagaaca cgatctcgaa ctgatcacga tcgcgatcga
     9241 aatcgctcag aatgaaattt tcgcacgagt ggctgaacac tagtgcgcgt gagcgcaggc
     9301 gatccgacag gggaagcttt gtaagatctc ttcccgagct gatcttgaag actctggcca
     9361 acggacggcc atcatccgac ggttccacga gtcgataact gggaagatgc tttttcaaac
     9421 cctcggtgaa gaatacggcc tcttctgctg atcccatcca ggcaatgcga ctgaaggcaa
     9481 actcccatcg ttgctcatat cgcggaaaca gtttctggaa cgcggcatgt gctgccagaa
     9541 agaattcccg gtcgatcgca cgatgcctgt cgatccactg tcgtgccaga ggcagaagcg
     9601 cgtgcccgta tcgattgatc gtgtcgcgcg cataccactg cggatcatct gacccgatag
     9661 ccctcgaata gagcccgttt ccgagagccg ttgccagacc ttcgttgagg tagactccgg
     9721 tgagaaagcc tgtcgcatca tcgttcgtct tgagatggtc gcgaatgtgc gagccaatcg
     9781 tgcgtgtggt gaagaattga tgagagagtt cgtgcagggc
//
LOCUS       DAORHD010000014         9676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6417_length_9676_cov_3.603783, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000014 DAORHD010000000
VERSION     DAORHD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6417_length_9676_cov_3.603783"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4438
                     /locus_tag="PKO06_00710"
     CDS             <1..4438
                     /locus_tag="PKO06_00710"
                     /inference="COORDINATES: protein motif:HMM:NF036483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV68186.1"
                     /translation="LYGSDRLKIPFGKHPLESRLKWSGITARPREEDYYKGILPVMED
                     ILKRDRNPSILRFVRSRTCQACQGARLNAQALAVEFHTWTIDRLSALSLNSLVEQLGH
                     FSLNDRERTIADPIIGEMQKKLTRLCSLGLGHLTLDRPSASLSAGEYQRVRLALQAFT
                     DLRHLLYVLDEPSLGLHPRDQRQLLDLLFHLRDAGNTILAVEHRRDAVLAADWLVDLG
                     PGAGPQGGELLYEGPLSPFLASGSFIDSPTRHWLTGTVPAGADAVDVSPTPAEAGRPA
                     LQVLGAAQHNLKQIDVCFPLQKLIAVTGVSGAGKSTLVHGILARSLRRSLHRAGSEPG
                     AHREILGLEFLDKVIAVDQQPIGRTSRSNPATYTGVFDLIRDLYARQPLARTRGWSKS
                     RFSWNVAGGRCEACQGTGTQEIGLHLLDPVEVVCEACGGQRYLPETLEVTWNGLSIAA
                     VLSLSVREARVHFDDQKQIMAHLDAMNDVGLDYLSLGQSSTTLSGGEAQRLKLATELA
                     RPGTGRTLYILDEPAAGLHHADIERLATVLRRLVSAGNTVIAVEHHPEFLLTADWIID
                     LGPEGGERGGHIVATGTPWEILKCPTSFTGQALKRLWQTSDVAKPAPEPSSPSLTPPT
                     EQRSPAPHLPSGIHLQDVRTHNLRDVQAFFPDRQLTVVTGVSGSGKTSLVMDTLYAES
                     HRRYVESLSTYARRFIQQSERPQLGAATGLTPTIAVTNRRGTANPRSTVGTMSGCLDL
                     WRVLMARSGERFCPECGSTLQRNVCAAGHFVGTAPLLARHFSFNDVLGACDHCHGLGQ
                     VAVCDIDRLVRFPERSLLDGALDGTRIGRFYGERDGQYVATLRAAGASLGHDFSLSWR
                     DLTPEARQIAMHGCGDREFEVTWQYRRGKRTGEHHMITAWKGFVSLIQEEFDRKRADE
                     RVNEFAELMVELPCAVCNGDRLKPEFRAVRWQGRSLGEWCRSSLTSLLDAGTDFLNRH
                     GAHQKGTPESESLTATETAARALIEALLPRLHQLVAAGLGYLSLDRTVQSLSGGEFRR
                     FLLACQLSSSLTGVTYVLDEPTVGLHERDTDRLLGLLHDLRRAGNTVVVIEHDPEVIR
                     AADHLIEVGPGAGSEGGTILATGTWPQIREHAQSLTARWLTDSAPWPIVTAHHLIHPG
                     VLIRGARAHNLKRLDLDIPLGSLVALTGVSGSGKSTLIEDVLLPSLTARHPINCDACS
                     SSVQFHRLLLVDQQLPSGSENSLVATITRVLDPIRTCFASTDDARSHGIGADAFSPFG
                     SVGRCETCQGSGGERVELDFLAAVRLVCEACEGKRFNHDVLGCRYREQSIADVLAMTV
                     SKASTFFSDQPEILARLEPLQRVGLVHLTLDRTTSSLSAGERQRLRLALALTDAHEAG
                     VSNHPTLFLFDEPTTGLHLADVARLFEVFQGLLTAGHSLIVVEHHLELIRRSDWVIDL
                     GPEGGEAGGNLVFAGPPADLAHHPTSATGIALRTSACGDGEV"
     gene            4527..5522
                     /locus_tag="PKO06_00715"
     CDS             4527..5522
                     /locus_tag="PKO06_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV68187.1"
                     /translation="MKRRVRTICLLAAIFVSIICVRVLTASPGSGDLTIAVISDLNES
                     YGSTTYSEHVDTALRFIEKRPPDLVLCAGDMVAGQKKSLSEETLVAMWSGFNRLVRQR
                     LNTAKIPFAFAFGNHDGPGTPAFAHERRIAEAFWLKRKPPLNFVDDSRFPRHYSFTCG
                     AVFFAVIDVAHPVLDDRQRLWLEQQLRLESARSARVRVILGHVPLYAIAEGRNKTGEV
                     MSDADRVHALFRRLGVDYYISGHHHAFYLSEKDGLKMISCGALGGGPRKLLGSDLPPR
                     KTLTFLTLLPQEHHFRIETYEVTDQPKLIDSSSLPDELHGFNGSSRRLVLPRQGQ"
     gene            complement(5516..6910)
                     /locus_tag="PKO06_00720"
     CDS             complement(5516..6910)
                     /locus_tag="PKO06_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HNV68188.1"
                     /translation="MPVLIVLGSLGFLMTIAYRGYSVILFAPVAALGAVMLTDPLLVP
                     PMFSSLFMDKMVGFIKLYFPVFLLGAVFGKVIELSGFSKSIVGAVIRLCGQERAMPAI
                     VLVGALLTYGGVSLFVVVFAVYPFAAEMFRQSNIPKRLIPGTIALGAFTFTMDALPGS
                     PQIQNIIPTTFFKTTTWAAPWLGVVGAVFILTVGLSYLNWRRQQAIRAGEGYGEGHSQ
                     EPTASVDASQLPSPLIAITPLVLVGVLNKVLTDRIPVWYGTTHSFLPAGAKAELVTDV
                     SKVAAIWAVEGALLVGILSVFAFAFRRVCEGFAEGSRAAVAGSLLASMNTASEYGFGA
                     VIAALPGFLLVASALQSISNPLLNEAITVTALAGITGSASGGMSIALAAMSDHFLQQA
                     SAAGISPEVLHRVAAMSSGGMDTLPHNGAVITLLTVTRLTHRQAYGDIFAITLVKTCA
                     VFLVIGFYYLTGIY"
     gene            7338..7751
                     /locus_tag="PKO06_00725"
     CDS             7338..7751
                     /locus_tag="PKO06_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68189.1"
                     /translation="MNKFLLVVVMAFLAASVVGLQAADITPFLKVTNYTSHMKTATAN
                     LEKIFMNDQIEFTGEARKIYIEAWKTCQSRTSYPTGSGSLAYMASSNTRDALNKAVEA
                     LDTLVYDQLVKFKNPEAKQLYREALKRMRLAAECL"
     gene            complement(7890..8321)
                     /locus_tag="PKO06_00730"
     CDS             complement(7890..8321)
                     /locus_tag="PKO06_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68190.1"
                     /translation="MRIKFLAVLALLVFCVAVSVLAKPPAPPSPYNLSFQLVNNLPHP
                     VTFMVGMRVFLETASDFYKKDLADENFAFNVEVPANGRVTHNKVLDGRWNSYVVQHLF
                     SVSVWGNGRELGNGQDRAANCKNFVITANPTAPVVSISRSN"
     gene            8530..>9676
                     /locus_tag="PKO06_00735"
     CDS             8530..>9676
                     /locus_tag="PKO06_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV68191.1"
                     /translation="MTLPKIGYDIGKESVRNNCFCFLRDHVEKHPERTCMKWVNTKNK
                     LWFDYNPFARTLTHDTFSYKNIHDMVVHVAAGYQKLGIKKGDMALVYVPMIPWLYAGM
                     FAFQMLGATCIFLDSFARADQLGYVADLCKPKVWLSGERGFAMGKGQPGLEAIPIKIN
                     MGPCTQTWTARFEDLLETREVAEPVAVAQEHTALITFTTGSSGTPKGADRSHRFLAAQ
                     HYAIDAFLPYNETDLDLPVFPIFSLNNLAAGVPTVLPAINVAEPANNDPYVLFTQIKG
                     EKITTLTLNPSLLNGLNKYCLEKGETLPELRRVAAGGAAVSRDVVAEFKKIAPNAELV
                     VMFGSTEAEPMAHITGTEMLAQRSVTEDDPELVDAGVNVGHFAEGLQY"
BASE COUNT         1954 a         3059 c         2715 g         1948 t
ORIGIN      
        1 cttgtacggc tcagatcgcc tcaagatccc gttcggcaag cacccgctcg agtcgcgtct
       61 gaaatggtcg ggtatcaccg cccgcccacg cgaagaagac tattacaagg gcattctgcc
      121 ggtgatggaa gacatcctca aacgtgatcg gaatccgagt atcctgcgtt tcgtacgcag
      181 ccgaacctgt caggcgtgcc agggggctcg tctgaatgcc caggcgctgg ccgtggagtt
      241 tcacacctgg actatcgacc gcttgagcgc tctcagtctc aactctctcg tggaacagct
      301 cggacatttt tctctgaacg atcgcgaacg aacgatcgct gaccccatca ttggcgagat
      361 gcagaagaaa ctcacccgtc tgtgcagtct ggggctcggg cacctgactc tcgatcggcc
      421 ttccgcctct ctttcggccg gagagtatca gcgcgttcgc ctggcactgc aggccttcac
      481 cgatctgcgg catcttctgt atgttctcga cgaaccgtcg ctgggtctgc accctcgcga
      541 tcagcggcaa ctgctcgatc tgctctttca cctgcgcgac gcgggcaata ccatactggc
      601 cgtggaacat cggcgtgatg ccgttctggc cgccgactgg ctggtcgatc tcggcccggg
      661 cgccggccct cagggcggag agcttctcta cgaagggcct ctgtctcctt ttcttgcatc
      721 gggaagcttc atcgatagcc cgactcggca ctggctgacc ggtactgttc cagccggcgc
      781 ggacgccgtc gatgtgtcac ccacgccggc ggaggccggc cgcccggcac tgcaggtgct
      841 gggagccgcc caacacaatc tgaaacagat cgacgtttgc tttcctctgc agaaactgat
      901 cgccgtcacc ggcgtttccg gtgccggaaa gtcgacgctc gtccacggca tcctggcccg
      961 gtcactgcgg cggtcactgc atcgagcggg cagcgaaccg ggggcacacc gcgagattct
     1021 tggcctcgag tttcttgaca aggtcattgc ggtagatcag caaccgatcg gtcgcacctc
     1081 gcgttcaaac cccgcgacgt ataccggcgt gttcgatctg attcgggatt tgtatgcccg
     1141 acagcctctc gcgcgcaccc gcggctggag caaaagtcgt ttttcatgga acgtcgcggg
     1201 cggccgatgc gaagcgtgtc agggaaccgg aacccaggag atcggcctgc acctgctcga
     1261 tccggtcgaa gtggtctgcg aggcctgcgg cggacagcgg tatctgcccg aaacgcttga
     1321 agtgacctgg aacggtcttt cgatcgccgc cgtgttatct ctttccgtcc gagaagctcg
     1381 tgttcatttc gatgatcaaa agcagattat ggcgcatctc gatgccatga acgatgtcgg
     1441 gctcgactat ctctcactgg ggcagtcttc gacgacgctt tccgggggtg aggcgcaacg
     1501 tctcaaactg gcgaccgaac ttgctcgccc cggcaccggc cgtaccttgt acattctcga
     1561 cgagccggcg gccggcctgc atcacgccga catcgagcga ctggcaacgg ttctgcgtcg
     1621 tctggtgagt gccgggaata ccgtcatcgc cgtcgaacac caccccgagt tcctgcttac
     1681 cgccgactgg atcatcgatc tagggccgga aggcggcgag cgcgggggac acatcgtcgc
     1741 cactggtacg ccgtgggaga tcttgaagtg cccaacttct ttcaccgggc aggcactgaa
     1801 aagattgtgg cagacgtctg acgtcgccaa gcccgcaccg gaaccttcct cgccgtctct
     1861 tacaccaccg acagagcaga ggtcaccggc cccgcacctt ccttccggca ttcatctgca
     1921 agacgttcgc acacacaacc tgcgcgatgt gcaggcattc tttccggatc gccaactcac
     1981 ggtcgtcacc ggcgtttcgg ggagcgggaa gacatcactg gtcatggata cgctctacgc
     2041 cgagagtcac cggcgttatg tcgagtcgct ctcgacgtat gcccgccggt tcattcagca
     2101 gagtgagcgg ccacaactcg gtgccgcgac cggcctgaca ccgacgattg cggtcaccaa
     2161 tcggcgcggc accgccaacc cccgctcgac cgtcggcacc atgagcggct gtctcgatct
     2221 ctggcgcgtg ctcatggccc gctccggtga gcgcttctgc cccgagtgcg gttcgacttt
     2281 acagagaaac gtctgcgccg ccgggcactt cgtcggcacg gctcccctgc tggctcgtca
     2341 cttttctttc aatgacgtgc tcggagcttg tgaccactgt cacggactag gacaagtagc
     2401 ggtgtgcgat atcgatcgat tggtgcgttt tcccgagcgg tctctgctcg acggcgctct
     2461 cgacggcacc cgtatcggcc gtttctacgg cgagcgcgac gggcagtatg tcgcaaccct
     2521 gcgggctgcc ggtgcctctc tcgggcacga tttttcactg tcgtggcgag acctgacgcc
     2581 cgaggcgcga cagatcgcca tgcatggttg cggcgatcgt gagttcgagg tgacgtggca
     2641 gtatcgtcgc ggcaaacgta ccggcgagca tcacatgata accgcatgga aaggatttgt
     2701 gtctctgatt caagaggaat tcgaccgaaa gcgcgcagac gaacgagtga acgagttcgc
     2761 cgaattgatg gtggagcttc cctgcgccgt ctgcaacggc gatcgtctga agcctgagtt
     2821 ccgcgccgtt cgctggcaag ggcgatctct gggcgaatgg tgtcgatcga gtctcaccag
     2881 ccttctcgac gccggcaccg attttctcaa ccgacacggc gctcaccaga agggaactcc
     2941 tgagagcgaa tctctgacag caacggaaac ggcggcccga gcgctcatcg aagccttgct
     3001 tccccgactc catcaactgg tggcggctgg tctagggtac ctgtcgctcg atcgtacagt
     3061 ccagtcgctt tccggcggcg agttcagacg gtttctgctg gcctgtcagc tgtcgagttc
     3121 actcaccggc gtcacctatg ttctcgatga gccgaccgtc ggtctgcatg agcgtgacac
     3181 cgatcgtctc ctcggactac tgcacgacct gcgccgggcc ggcaacaccg tggtcgtcat
     3241 cgaacatgac cccgaagtca ttcgtgccgc cgaccacctc atcgaggtcg gccccggcgc
     3301 gggctcagag ggcggaacga ttctggccac cggcacctgg ccgcagatcc gcgagcacgc
     3361 gcaaagcctg acggcacgct ggcttactga cagcgcgccg tggccgatcg tcacagcgca
     3421 ccatctgatt catcccggcg ttctgattcg cggagcacga gcgcacaacc tgaagcgtct
     3481 tgatctcgac ataccactcg ggagcctcgt cgccctcacc ggtgtttccg ggtctggaaa
     3541 gtccactctg atcgaagacg ttctgcttcc gtctctgacc gcgagacacc cgatcaactg
     3601 cgatgcgtgt tcctcgtctg tccagtttca tcgactgttg ctcgtcgacc aacagcttcc
     3661 atcgggatct gaaaacagtc tggtggcaac catcacgcga gtgcttgatc cgattcgtac
     3721 gtgttttgcc tcgaccgatg acgctcgcag tcacggaatc ggcgctgatg ctttctcacc
     3781 tttcggctcg gtcggtcggt gcgagacctg tcagggcagt ggcggcgaac gagtcgaact
     3841 cgattttctg gcggcggtgc gccttgtctg cgaggcttgc gagggcaagc gcttcaatca
     3901 cgacgtgctc ggctgtcgat atcgggaaca gagcatcgct gacgtcctgg cgatgaccgt
     3961 ctcgaaggct tcgaccttct tctctgacca accggaaatt ctcgcgcgac tcgaaccatt
     4021 gcagagagtc ggtctcgtgc atctgactct cgatcgcacg acatccagct tgtcagcggg
     4081 agaacgtcag cgtctgcgcc tggcactggc actcaccgat gcccatgagg ccggcgtctc
     4141 caaccaccca accctgtttc tgttcgacga gccgaccacc ggcctgcatc tcgccgatgt
     4201 cgcgcgactg ttcgaagtgt tccagggact gctgaccgcc gggcattcgc tcatcgttgt
     4261 cgagcatcac ctggagctga tcagacgctc cgactgggtc atcgacctcg gccccgaagg
     4321 cggcgaagcc ggaggcaacc tcgtattcgc cggcccccct gccgatctcg cacaccaccc
     4381 gacctcagcc accggcatcg ctctgcgcac ctctgcctgt ggcgacggcg aagtgtgacc
     4441 taggaggttt cgtccatgcg tatgttgttt ctccagggca catgcagacg accgaagcgg
     4501 tctgccatcg cgaaagggaa ccatccatga aacgccgggt tcgcaccatc tgtcttctcg
     4561 cggcgatctt cgtcagcatc atctgcgttc gcgttctcac cgcatccccc ggttcaggcg
     4621 atctgacgat tgccgtcatc tctgatctca atgaatccta tggttcgacg acgtacagcg
     4681 agcatgtcga taccgcactg cgtttcatcg agaaacgccc ccccgatctg gttctgtgcg
     4741 ccggcgacat ggtggcgggg cagaaaaagt ccctgtcaga agagacgctc gttgccatgt
     4801 ggagcgggtt taaccgcctg gttcggcaac gattgaacac ggcgaagatc cctttcgcct
     4861 tcgcgttcgg caaccacgac ggccccggca cgccggcttt cgcgcatgaa cgacgcattg
     4921 ccgaggcgtt ttggctcaag cggaaaccgc cgttgaactt tgtcgacgat tcgcgctttc
     4981 ctcgccatta cagtttcacc tgtggtgccg tgttcttcgc cgtcatcgat gtcgcgcatc
     5041 cggttctcga tgatcgccag cgtctctggc ttgaacaaca actgcgactc gaaagcgctc
     5101 gttccgcgag agtccgagtc atacttggcc acgtacctct ctacgcgatt gcagaaggca
     5161 gaaacaaaac aggtgaggtc atgagcgacg ccgaccgggt ccatgcactg ttccgtcgcc
     5221 tcggggtcga ttactacatc tcaggccacc accacgcctt ctatctttcc gagaaagacg
     5281 ggctcaagat gatctcatgc ggcgcgctcg gcggcggacc tcgaaaactg ctcgggtccg
     5341 atctgccgcc gcgcaagacc ctcaccttcc tgacgctctt gcctcaagaa caccactttc
     5401 gcatcgagac gtatgaggtc accgaccagc cgaagctcat cgattcgtcc agtcttcctg
     5461 acgagcttca cggcttcaac ggctcatctc gccgacttgt tcttcctcgc cagggtcagt
     5521 agattccggt gagatagtaa aaaccgatca ccagaaagac ggcgcaggtc ttgaccagcg
     5581 tgatcgcaaa gatgtcgccg tatgcctgcc gatgtgtgag cctggtcacc gtcagcaggg
     5641 tgatcaccgc cccattgtgc ggcaacgtgt ccatgccccc actcgacatc gcagccaccc
     5701 gatgcagaac ttcgggcgag atgcctgccg ctgaggcctg ctgcagaaag tgatccgaca
     5761 tggccgcaag cgcgatactc attccgcccg acgccgagcc ggtgattccc gccagagccg
     5821 tcaccgtgat cgcttcattg agcagaggat tcgagatgga ctgcagagct gaagcgacca
     5881 gcagaaaacc tggcagggcg gcgatcaccg ctccgaaccc gtattctgaa gccgtgttca
     5941 tgctcgccag cagcgacccg gccaccgcgg cacgcgagcc ttcagcgaaa ccttcgcaga
     6001 ctcggcggaa cgcaaatgcg aacaccgaaa gaatgccgac cagaagagca ccttcgaccg
     6061 cccagatcgc ggcgaccttg ctgacatcgg tcaccagttc cgctttcgcg ccggccggaa
     6121 gaaacgagtg cgttgtgccg taccagaccg gaatgcgatc ggtaagcacc ttgttcagca
     6181 caccgaccag aaccagtggc gtaatcgcga tcagcggcga gggcagctgt gaggcgtcga
     6241 ccgaggcggt cggctcctgc gaatgccctt ctccgtatcc ttctccggct cttatcgcct
     6301 gctgtcgacg ccagttgaga taggacaatc cgaccgtgag aatgaacacc gcgccgacga
     6361 caccgagcca gggagccgcc caggtcgtgg tcttgaagaa cgtcgtcgga atgatgttct
     6421 gaatctgcgg gctgcccgga agcgcatcca tcgtgaacgt gaaggcacca agcgcgatgg
     6481 ttcccggaat cagtcgtttg gggatattgc tctgacgaaa catctcggcc gcgaacggat
     6541 agacggcaaa caccacgacg aaaagcgaga cgccgccgta ggtaagcagt gcgccgacca
     6601 ggacgattgc cggcatggcc cgttcctgtc cgcagagccg aatgaccgct ccgacgatcg
     6661 atttcgaaaa gcccgacagt tcaatgacct tgccgaacac cgcaccgagc agaaacaccg
     6721 gaaagtacaa cttgatgaag ccgaccatct tgtccatgaa caggctgctg aacatcggcg
     6781 gcaccagcag ggggtcggtc agcatcacgg cgccaagagc cgcgaccggc gcgaacagaa
     6841 tcacgctgta gccgcggtag gcaatggtca tcagaaatcc gagcgagcca agaacgatca
     6901 gaaccggaat catgaggtct ctcccctggt gctcgccaga tgaaaccgaa ccggatcaac
     6961 cgacgaggcg acgatgattt ttgtgcaatg cacaatatca caatccacca acccggtcga
     7021 gaacaatcgc cttttaccag aacccggctc tcaactcacg tatcgccgtt cacgcagcaa
     7081 agcacgttcc tcacgaggcg cgtttcttcc ccttgctctt tcgtctcttt ttccggggaa
     7141 ctgaggggtc gggaagctgt gcgaatgcag gaccattggc tcagcagttc cctccacgga
     7201 ttgactggac gagctgttca cccgtcctca gagaattcag tggccgagct gccctgctga
     7261 aacaactttt cagaaacacc ctcggttccc gccagatatg gtattctagg ggcgagatcc
     7321 atttttctgg aggaaacatg aacaagttcc tgttggtcgt tgtcatggcc tttcttgccg
     7381 cctctgtggt cgggcttcaa gccgcggaca tcaccccgtt tctcaaggtc acgaactaca
     7441 cttcgcacat gaagacggcg accgcaaacc tcgaaaagat cttcatgaat gaccagatcg
     7501 agttcaccgg ggaggcccgc aagatctaca tcgaagcctg gaaaacctgc caaagccgga
     7561 cctcttaccc gactggtagt gggtctttgg cttacatggc gtcctccaac acccgcgatg
     7621 ctctgaataa agcggttgaa gcactcgaca ccctggtcta cgaccagctg gtgaagttca
     7681 agaaccccga agccaagcag ttgtaccgcg aagctctcaa gagaatgcgc ctcgctgccg
     7741 aatgcctgtg agcgtcaagc gctaactcat accgttctcg ctcgcgttcg agaacccaag
     7801 ccacttcttc ggaagtggct tttcacttgc ggagacaatc gatgaaatcg gcactctgat
     7861 gaggtatcgt cgaccaccct caccctctct cagttcgatc tgctgatgct cacgaccggt
     7921 gcggtgggat tggcggtaat cacaaagttc ttgcaattgg ccgcgcgatc ctggccattc
     7981 cccaattctc tcccgtttcc ccagacactg accgaaaaca gatgctgaac gacgtagctg
     8041 ttccatcttc cgtccaggac cttgttgtgg gtcactcgcc cattcgcggg gacctcaacg
     8101 ttgaaggcga aattttcgtc ggccagatct ttcttgtaaa agtccgaagc ggtttcgaga
     8161 aaaacccgca tgccaaccat gaaggtcacg ggatggggca ggttgttgac caactgaaaa
     8221 gaaaggttat acggcgaagg tggcgctggg ggcttggcca acacagaaac ggcaacgcag
     8281 aataccaaga gagccagaac ggccagaaac ttgattcgca cgattgattc tccttaatgt
     8341 cttcacactg atccttggaa ctagtctaac acttctcgtt tttcggttca aagcattctc
     8401 cgtcgagcag gcaacggctt tgttgcccgg gaaaaatcag gagtggtatc ctgcacgtat
     8461 tcgagttcgt tcctgcccat gatggctcgc aggcacaggc agatgttatg caacaacaag
     8521 gagtcgacaa tgacgcttcc gaagattggc tacgacatcg gcaaagagag tgtgcgcaac
     8581 aactgtttct gcttcctgcg agatcacgtc gagaaacacc ccgaacgaac ctgcatgaag
     8641 tgggtcaaca ccaagaacaa gctgtggttc gactacaacc cgttcgcccg caccctgacg
     8701 cacgacacgt tctcatacaa aaacattcat gatatggttg tacatgtcgc cgccggctat
     8761 cagaagctcg gtatcaaaaa aggcgacatg gccctggtct acgtgccgat gattccgtgg
     8821 ctctatgcgg gcatgttcgc ctttcagatg ctgggggcga cctgcatctt cctcgattcg
     8881 ttcgcgcggg ccgatcagct cgggtatgtc gccgatctct gcaagcccaa agtctggctg
     8941 tcgggcgagc gcggcttcgc catgggcaaa ggccagcccg gcctcgaagc cattccgatc
     9001 aagatcaaca tggggccttg cacgcagacc tggaccgcgc gcttcgaaga cctgctcgaa
     9061 acccgcgaag tcgccgagcc ggtcgcagtc gcgcaggagc ataccgcgct catcaccttc
     9121 accaccggtt cttccggtac gccgaagggg gctgaccgtt cgcatcggtt cctggccgcc
     9181 cagcactacg ccatcgacgc ctttctgcca tacaacgaga ccgatctcga tctcccggtc
     9241 tttccgatct tttcactgaa caacctcgcg gcgggcgtgc ccacggttct gccggccatc
     9301 aacgtggcgg agccggccaa caatgacccc tacgtgcttt tcacacagat caagggcgag
     9361 aagatcacca cgctcacgct gaacccttcg ctgctcaacg ggttgaacaa gtactgcctc
     9421 gagaaaggtg agacgctgcc cgagctacgg cgcgttgccg ccggcggggc cgccgtatcg
     9481 cgcgatgtgg tcgccgagtt caagaagatt gccccgaacg ccgagctggt cgtcatgttc
     9541 ggctcgaccg aagccgagcc gatggcgcac atcacaggca ccgagatgct ggcccagcgc
     9601 agcgtcaccg aagatgaccc cgaactcgtc gacgctggcg tcaacgtcgg tcacttcgcc
     9661 gagggtctgc agtaca
//
LOCUS       DAORHD010000015         9661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6431_length_9661_cov_4.050281, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000015 DAORHD010000000
VERSION     DAORHD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_9661_cov_4.050281"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="whiA"
                     /locus_tag="PKO06_00740"
     CDS             complement(<1..778)
                     /gene="whiA"
                     /locus_tag="PKO06_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNV68192.1"
                     /translation="MPTRRYLRRLKHALWHASFPFDCCRYTFLALALDAVAEDTGRLP
                     RRLYRLLGRTVRDTALLGEDTRTPPEGKTMIVQPIDIHSLRRLIMTRTAPNASQPLPP
                     HCQKAAIAGLFLRCGYMQDPQSGYHAEFAVKERWRARLVYRLGHLLRLPFHVLTRRDR
                     VVFYLKDRRHLLRLLRRLDQHDLVMELQDLVSTRDLLGLVNRQVNFETANINKSVAAA
                     EQQIERIRRLLAHPDQGIWSPALRELALLRLRFPHDGYEGL"
     gene            complement(787..2079)
                     /locus_tag="PKO06_00745"
     CDS             complement(787..2079)
                     /locus_tag="PKO06_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNV68193.1"
                     /translation="MIRFSLRRLVGPDLRSRRWVFFSIFGVLFSTLALVITTLVFLLL
                     APAGLPTVESFHPLGILFAALSAFLLGWYWLLKGYYHALTLLSMADQDAPSRLREVMF
                     RPSSRTDRLPSLITIGGGTGMSSILKGLRELPVDLTAIVTVADDGGSSGRLRKEMQIL
                     PPGDIRNCLVALSRSESLLSRLFQYRFHDGGAASGHSFGNLFIAAMSDLLGDFGSAVR
                     EASNILAIKGKVLPVTCENVQLVARFEDGSEIMGESAITGAQKRIAAIELRPAAALAN
                     PEALAAIDRADLIVLGPGSLFTSVIPNLLVPEVADRLNRSQARIVYICNVMTQPGETD
                     GYSAFDHVRKILDTTPLKRIDVAIVNSRRAARTLMTRYEEKGQYWVPPTVTPIEGMGV
                     RVITGDFLSETDLVRHNSLSLAQVLAEMLRERPATPVH"
     gene            complement(2103..3023)
                     /gene="hprK"
                     /locus_tag="PKO06_00750"
     CDS             complement(2103..3023)
                     /gene="hprK"
                     /locus_tag="PKO06_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNV68194.1"
                     /translation="MALVLVRELMRDLGLTNIAVQGDLDRKIISSEVHRPGLELAGFF
                     EHFGYERVIVLGRTELAYLGEQTVEVRKVRLRQLLFFNIPCIIVTDDLLVLDDLIELC
                     NQKQIPILRSAEHTGEFIYQLSRYLHHRFAPRKSMHGVFVEVFGVGILITGPSGIGKS
                     ECALELIKRGHRLVADDAVQLMKISDTKVIGYPDEMIRHHMEIRGLGIIDIRSLFGIA
                     ATADEKPVEMVIHLEKWDDRKDYDRLGILQKTIRLLKVEVPHTIIPVREGRNLAVVVE
                     TAAMNFYLRKMGIFTAEEFAKKLQAKMAKD"
     gene            complement(3071..4330)
                     /locus_tag="PKO06_00755"
     CDS             complement(3071..4330)
                     /locus_tag="PKO06_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68195.1"
                     /translation="MPAEELFPRSPLITFIGILISFLCVQMLLQTTFLSTLPRTMAFT
                     TGTMLIMTMLVTLSSRIGSRILRHFGYLLPGVWLWFFPFLSLLFSHAFFFVLIKPIPD
                     LLGRDCWTRILGLNAKCTTLAISSAFSGTMFFLALYLIIGAFRFLERTMAAASELEDQ
                     LLAEVSRTSARSPGSPPAPDTAALAPAETLPTVKEQLQAMEAGRHRARSFNRVAILVL
                     FVCLAVMASWVVFARPETILYYRGMMQLFSRQHPEHALAAFQHLAQKYPTYTYLDTVL
                     FQAAWTLDRRLGRTPEAMQAYREFLDRFGVNNVWADDVTANLVRLALDKQANATETLH
                     WTNLYQQQFPEGPFSPHILLYEMRALLQLGRTDEARQKQQQALSRFGESSIPLYNSED
                     DFMSLLPFRMVLESLIEPATTSEPTSR"
     gene            complement(4395..5465)
                     /locus_tag="PKO06_00760"
     CDS             complement(4395..5465)
                     /locus_tag="PKO06_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68196.1"
                     /translation="MVTSSAAPSVSPDGLFASTLIRYLLNQPLPLSHSLQNRQQLEQI
                     TDDLRQALSRRVRGLPSTSPGHTLTAGGTLLYWLGFYAGFTAAPVLGFAIALLGSAGI
                     SAGYKFTCEAWRNFEYLRLFYTSLALMQNADHTITEQERRILSDFLLSLELSDAEKNQ
                     LREITAPTLEDIKVPEWVDTDQRRSILSACWSLVYCDGVQPSEGDLFSRLSERFDVSA
                     EEATLLCRQAEKLIDRQEEDVLNLGRLYKSLTQQPEIPNALLEALIHSSVKWPSREAF
                     AETLTSSPVTAPTLPTAAREAQFLLAGSTVLDLLLGQHPELAAAGILTPLTQLARAVG
                     RETELAEFHALCAGLFHRLNAN"
     gene            5659..7980
                     /locus_tag="PKO06_00765"
     CDS             5659..7980
                     /locus_tag="PKO06_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNV68197.1"
                     /translation="MRNLCLMAVAIVLCLWVFTGSALAQEPGPEGGAPAGAAFQDLPK
                     DHWAFRDVEFLVKSGYMEGYPDGNFKGRKVVTRYDVALILARMMKRIEDRKQSANDAT
                     EAERAALARLTKEFRDELGLLGVRVDALERRMNDVEAKADAVEKALPKVNISGFYRGR
                     GQMIFDPTTVGRDEAGYSATFTKSGLVTFYQTMYLRFTGKPLGEKIETFYELLGYMSG
                     RNWNRLVYNDAGKTSGPNPFDRVDDYISTVEYQRYAQSNKLHFISNARSAKVRVYAGE
                     AVTGINDPLNLLTEDTDIIEPYQGLEITGSDRGLTYQGQVHKYDMRQGSTNTHEMFAG
                     RLVWKLPAKFSPDALSIGTSFGEKVHDYKIRGNSNTVRGVDVNYTTERVGKVQATAEF
                     LTSTDYHTDTRDNTKRNLGDEGTKFDVSLQQGGFTGTLKHYDFGREFRAYMAPIWAYD
                     IGDEDDNSNYPYSPIFPDNYGHNGFYGEKLTRWSANYDFGNKLLSIAKNLSMEATWLS
                     KTWEVDPFKPQDTDGYSGRKFTYQVLADFTDNTTLKYDFEQKFKALAKSQGRYSNTLE
                     LNLKLNDSVGTKAKINVINDQDERDIVGTTEYERNDRTGYFEVNSNINPRVFAKGSVE
                     HQVRWVNAPKEDIRIDYIGETTYNFTPTLSFTAGAQHVDFESLGEPGKSSLANAILGE
                     LKKNFTDKFRGRAFYTRAVVDYKDGETDTIDRENIYGELIYDISKDASFKLKFGYDYP
                     NDGRWDISSVGNGRDFKGIETQKMMIFEAKTNF"
     gene            7992..9167
                     /locus_tag="PKO06_00770"
     CDS             7992..9167
                     /locus_tag="PKO06_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68198.1"
                     /translation="MKRKLLLLLALSVAMLATGCIQEKLFIPVATVTGKIVVPAGKDP
                     IGIKITVAGNPSITTYVNETGDYKLEFPKTGRYLLVARSKYYDVDYVWVDAFLEQTVK
                     ADNLSLKEKIVGEVKFLATIVDYPNAEWFKVKSITPVWATDTVSLYDDGTHDDRLAND
                     GIFTLRLTNLATGYQQYHLIWYGDKDGEIGENTEKLDPHREAERVGNSEMWIREPEVK
                     LARGRITSALTGVNYSEVTLATKGGARKMLVNSDGTYSIAMEGNGREYLVFRSVNFHV
                     RAIPVDLTTVPIYDVPDTVLKAKAPGEIKLVLVKNDFQDVLNPMVVGEFTDWHPEAMY
                     DDGTHGDEVSGDGVYTYTKSGVGPGYYKYAYNIREGNQVRDPYEESGDSSFSILTVK"
     gene            9170..>9661
                     /locus_tag="PKO06_00775"
     CDS             9170..>9661
                     /locus_tag="PKO06_00775"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HNV68199.1"
                     /translation="MMRRFTALVLILLVFVTVQAEAREYAPPTNYFYEAGSVFDVSDP
                     VGDDKGPGYYTYPLDSRLRRGTFDVTRFYAYEEGKVLTFVIQFRNYVMTEWPNGKGSE
                     DQGFVANLCDIYIDMNRQPGRGYNKALPGRQIEFADQMGWEKMILVTPLSQWRVYEQL
                     KDKT"
BASE COUNT         2214 a         2662 c         2928 g         1857 t
ORIGIN      
        1 cgagaccctc gtaaccgtca tgcggaaaac gcagtcgcag cagggccagt tccctcaggg
       61 ccgggctcca gatgccctgg tccgggtgcg ccagcagtcg gcggatgcgt tcgatctgct
      121 gctctgccgc ggccacggat ttgttgatgt tcgcggtttc gaagttgacc tgacgattga
      181 ccaggccgag cagatcccgg gtcgagacga ggtcctgcag ttccatgacc aggtcgtgct
      241 gatccagtcg gcgcagcaag cgcagcaggt gccgtcggtc tttcagatag aagaccaccc
      301 gatcacggcg ggtcagcacg tgaaacggca gtcgcagcag atgtcccaga cggtagacca
      361 ggcgggcgcg ccagcgttcc ttcacggcaa actcggcatg atagccggac tgggggtcct
      421 gcatgtagcc gcagcgcaga aacagtccgg cgattgccgc tttctggcaa tgaggcggca
      481 ggggttggct cgcgttcggt gccgtacgag tcatgatcag gcgtcgcagg ctgtggatgt
      541 cgatcggctg cacaatcatc gtctttccct cgggcggcgt gcgcgtatct tcgcccaaca
      601 gggcggtatc gcgcactgtg cggccgagaa gccggtacag acggcgcggc agtcggccgg
      661 tatcttcggc gaccgcatcg agagccagcg ccagaaacgt atagcgacag cagtcgaagg
      721 gaaatgaggc gtgccagagg gcgtgcttca gccgtcgaag gtagcgacgc gtcggcacgg
      781 ctcggctcaa tgaaccgggg ttgccggtcg ctcgcgcagc atctcggcca ggacttgagc
      841 cagactgagc gagttatgac gcaccagatc ggtttcggaa aggaagtccc cggtgatgac
      901 tctgaccccc atgccctcga tcggggtgac agtcggcggc acccagtact gtcccttctc
      961 ttcgtagcgt gtcatcagtg tgcgcgcggc acgtcgactg ttgacgatcg ccacatcgat
     1021 tcgtttcaag ggagtggtgt cgagaatctt gcgcacatga tcgaacgcgg aatatccgtc
     1081 ggtctcgccc ggttgcgtca tgacattgca gatgtagacg atgcgggctt ggctgcggtt
     1141 caggcggtcc gcgacttccg ggacgagaag attgggaatg acgctggtga acagactgcc
     1201 cggacccagg acgatcaggt ctgcgcggtc gatggcggcc agcgcttccg ggttggccag
     1261 cgcggcggcc ggtcgcagtt cgatggcggc gatgcgtttc tgcgccccgg tgatggccga
     1321 ttcgcccatg atttcgcttc catcctcgaa acgtgcgacc aattgaacgt tttcgcaggt
     1381 gacgggcagg acttttccct tgatggccag aatgttgctc gcctcacgca ccgcggaacc
     1441 gaagtcgccg agcaggtcgg acatggcggc gatgaacaga ttcccgaaac tgtgtccaga
     1501 ggcggctccg ccgtcatgga accggtactg aaacaggcgc gacaacagac tctccgatcg
     1561 tgacagggcc acgagacagt tacggatatc acccggcggc aggatctgca tttccttgcg
     1621 cagccggccc gaagacccgc cgtcatcggc gacggtgacg atcgcggtca gatcgaccgg
     1681 taactcacgc aagcctttca gaatggaact catgcccgta ccgccgccga tggtgatcaa
     1741 acttggcagg cggtcggtgc ggctgctggg gcgaaacatg acttcgcgca acctggacgg
     1801 agcatcctgg tcggccatcg agagcagggt cagggcatgg taatacccct tcagcagcca
     1861 gtaccagcca agaagaaagg cgctcagcgc ggcaaacaga atgccgagcg ggtgaaagct
     1921 ctcgacggtg ggcagaccgg cgggggcgag caggagaaag acgagtgtcg tgatgaccag
     1981 ggccagcgtc gagaacagca cgccaaagat cgagaaaaag acccagcgtc gactgcgaag
     2041 atcagggccg accaggcgac gcagggaaaa acggatcatg aacgaatggg gcccggtggg
     2101 gcctaatcct tggccatctt ggcctgcagt ttcttcgcga actcttcggc ggtgaagatg
     2161 cccatcttgc gcagataaaa gttcatggcg gcggtttcga cgacgacggc cagattacga
     2221 ccttcgcgca ccggaatgat cgtgtgcggc acttcgacct tcaagaggcg aatcgtcttc
     2281 tgcagaatgc ccagccggtc gtagtccttg cggtcatccc acttctcgag atgaatgacc
     2341 atctcgaccg gcttttcatc cgcggtggcc gcgataccga acagagatcg aatatcgatg
     2401 atgcccagac cgcgaatctc catgtgatgg cggatcatct cgtccggata gccgatgacc
     2461 ttggtgtcgc tgatcttcat cagctgcacc gcatcgtcgg cgaccagtcg gtgtccgcgt
     2521 ttgatcagtt cgagagcgca ctcgctcttg ccgattcctg acgggccggt gatgagaata
     2581 ccgacgccga acacctcgac gaacaccccg tgcatacttt tgcgaggagc aaagcggtga
     2641 tgcaggtaac gcgacagttg gtagatgaat tcgccggtat gttccgctga ccgcagaatc
     2701 ggaatctgct tctggttgca gagttcgatc aggtcgtcga gtacgagcag gtcgtcggta
     2761 acaatgatgc agggaatatt gaagaacagc agttggcgca aacgaacctt gcggacctcg
     2821 acggtctgct caccgagata agccaactcg gtgcgcccga gcacgatcac gcgctcgtag
     2881 ccaaagtgtt caaaaaagcc ggccagttcg agacctggcc gatgcacttc cgaagagatg
     2941 atcttgcgat cgagatcacc ctggacggcg atgttggtca gccccaggtc gcgcatcaat
     3001 tcgcgaacga gtaccagtgc catgaagagg tcctgtcagg tgtggtgtcg gcgcgagtat
     3061 accacatccc tcaccgggaa gtgggttctg aagtcgtcgc gggttcgatc aggctttcga
     3121 gaaccatgcg gaacggcagg aggctcatga aatcatcttc ggaattgtac aggggaatgg
     3181 aggattcacc aaagcggctc agggcctgct gctgcttttg tcgggcttcg tcggttctgc
     3241 cgagctgcag cagggctctc atttcataga gcagaatatg gggactgaag ggaccttccg
     3301 gaaactgctg ctggtacaaa ttcgtccagt gaagagtctc ggtggcgttg gcctgcttgt
     3361 cgagcgcgag tcgaaccagg ttggcggtga catcatcggc ccagacattg ttcacgccga
     3421 accggtcaag aaattcacgg taggcctgca tggcttcggg agtgcgaccg agtcgtcgat
     3481 cgagagtcca ggcggcttga aacagcaccg tgtcgagata ggtatacgtc ggatacttct
     3541 gcgccagatg ttgaaaggcg gcaagcgcgt gttccggatg ctgccgggag aacagctgca
     3601 tcatgccgcg ataatacaga atggtttctg gtcgcgcgaa gacgacccac gaagccatca
     3661 cggcgagaca gacaaagagc acgagaatcg ccacgcgatt aaatgagcgg gcccgatggc
     3721 gaccggcttc catggcttga agctgctctt tcaccgtcgg cagagtctct gcgggagcga
     3781 gagctgccgt gtcaggcgcc ggcgggctgc ccggagatcg cgcggaagtg cgtgagactt
     3841 ccgcgagcaa ctgatcttcc agttccgaag cggcggccat ggtgcgctcg agaaagcgaa
     3901 aagcgccgat gattagatac agcgcgagaa agaacatggt tcccgagaat gccgaagaga
     3961 tcgcgagggt cgtgcacttg gcgttcagac cgaggatcct ggtccagcag tcacgtccga
     4021 gcagatccgg aatcggcttg atcaggacga agaaaaacgc atgcgaaaac agcaggctca
     4081 aaaaggggaa aaaccacagc cagactcctg gcagcagata gccgaagtgc ctcaggatac
     4141 gggagccgat gcgggaactg agcgttacca gcatcgtcat gatcagcatc gtgccggttg
     4201 tgaaagccat agtacgaggg agagtcgaaa gaaaagtagt ctgtaaaagc atctgcacgc
     4261 agagaaacga aatgaggatg ccgatgaacg tgatcagcgg gctgcgggga aacagttctt
     4321 ccgccggcat cgacgaactc accgcctggg tgccgctggg ggatggagga accggattca
     4381 cgcgtggaaa acgcttagtt cgcgttcagg cgatgaaaaa gaccggcgca caaggcgtgg
     4441 aactcggcca gttccgtttc gcgcccgacg gctctcgcca gctgagtcaa gggggtgaga
     4501 atgcctgccg ctgccagttc ggggtgctgc ccgaggagaa ggtcgagaac tgttgacccg
     4561 gcgagcagaa actgggcttc acgggcggcc gttggcagcg tgggagcggt cacgggagaa
     4621 ctggtcagag tttcggcgaa tgcctcgcgc gaaggccatt tcacgctgct gtgaatgaga
     4681 gcctcgagga gtgcatttgg aatctcgggt tgctgtgtca gtgacttgta caaacgaccg
     4741 agattgagca cgtcttcctc ctggcggtct atcagctttt cggcttgtcg acagagcagg
     4801 gtggcttctt cggccgatac gtcgaaacgc tcgctcagtc gcgaaaaaag atcgccttcg
     4861 cttggttgta cgccgtcgca gtagaccagg ctccagcagg cagacagaat cgatcggcgc
     4921 tggtcagtgt cgacccactc gggaaccttg atgtcctcga gggtgggagc ggtgatctcg
     4981 cgcaactgat tcttttccgc atccgaaagt tccagggaaa gcagaaagtc ggaaaggatg
     5041 cgtcgttcct gctcggtgat cgtgtgatca gcattctgca tcagggccag cgaagtatag
     5101 aacagacgca gatactcgaa gttgcgccag gcttcgcagg tgaacttgta gccggccgaa
     5161 attcctgccg aacccagcag ggcgatcgcg aaccccagta ccggagcggc cgtgaaaccg
     5221 gcatagaacc ccagccagta cagcagagtt ccgcccgcgg tcagggtgtg cccgggagag
     5281 gtggatggga ggccgcgaac tcgcctgctc agcgcctgcc gaagatcatc ggttatctgc
     5341 tcgagttgct gacgattctg aagggaatgt gacagcggga gaggctggtt gagcagatag
     5401 cgtatcaacg tcgaagcgaa caacccgtct ggcgaaaccg acggcgccgc ggaggaagtc
     5461 accatgtgct gatctccatt gccacaggat agagacagtg tagcacgttt tgacggctca
     5521 cgaagatttt caccgtgaac gaattcggga aaacctcttc gcctttcgag gggaagtgtc
     5581 gataaccacc atagctcatt tcagccccaa gccaaccaaa agtaaacaag aatgaggcaa
     5641 acaaacaagg gttcgggcgt gcgaaacctc tgcctgatgg cagtggcgat agtcctctgc
     5701 ctgtgggttt tcaccggttc tgctctggcg caggagcctg gaccagaagg tggagcacct
     5761 gccggtgcgg ctttccagga tcttcccaaa gaccactggg cgttccgcga cgtcgagttc
     5821 ctggtcaaga gtgggtacat ggaaggctat ccggacggga acttcaaggg ccgcaaagtt
     5881 gtgacccggt atgacgtcgc cctgattctg gcccgcatga tgaaacgaat cgaagatcgg
     5941 aaacaatccg ccaacgatgc caccgaggcg gaacgcgcgg ctttagcgcg actgaccaaa
     6001 gagtttcgcg acgaactcgg cctgctcggc gttcgcgtcg atgcgctcga acgccgcatg
     6061 aacgacgtcg aggccaaggc tgacgccgtt gagaaggcgt tgcccaaagt caacatttcc
     6121 ggtttttatc gtggtcgcgg tcagatgatc ttcgatccga ccaccgtcgg ccgcgatgaa
     6181 gctggctatt cggcaacctt caccaagtcg gggttggtga ccttctacca gaccatgtat
     6241 ctgcgcttca ccggaaagcc gctgggtgag aagatcgaga ccttctacga actgctgggc
     6301 tacatgtccg ggcgcaactg gaaccgcctg gtctataacg atgccggtaa gaccagtggt
     6361 ccgaacccct ttgaccgggt tgacgactac atctctacgg tggaatatca gcggtacgcg
     6421 cagagcaaca aactgcactt catcagcaac gcccgctcgg ccaaggtgcg cgtctatgcc
     6481 ggtgaagccg tgaccggtat caacgacccg ttaaacctgt tgacggaaga caccgatatc
     6541 atcgagcctt atcagggcct cgaaattacc ggttctgatc gcggtttgac gtatcagggg
     6601 caggtacata agtatgacat gcgtcagggc tcgaccaaca cgcacgaaat gtttgctggt
     6661 cgactggtct ggaaactgcc cgccaagttc agccctgatg cgctgtcgat cggcaccagc
     6721 tttggtgaaa aagtccatga ctacaagatt cgcggcaact cgaacaccgt tcgcggcgtc
     6781 gacgtcaact acaccaccga gcgcgtgggc aaggtgcagg ccacggctga gttcctgacg
     6841 tcgaccgact atcataccga tactcgtgac aataccaagc gcaacctcgg cgatgaaggc
     6901 acgaagtttg acgtcagcct gcagcagggg ggcttcaccg gaaccctcaa gcactacgac
     6961 ttcggtcgag agttccgcgc ttacatggcg ccgatctggg cgtatgacat cggcgacgaa
     7021 gatgacaaca gcaactatcc gtacagcccg atctttcccg acaactacgg gcacaacgga
     7081 ttctacggcg agaaattgac gcgctggtcg gcgaactacg acttcggcaa caagctgctt
     7141 tcgatcgcca agaacctgtc gatggaagcg acctggttga gcaagacctg ggaagtggac
     7201 ccattcaaac cgcaggatac cgatggctac tccggccgca agtttaccta tcaggtgttg
     7261 gctgacttca ctgacaacac cacgctcaaa tacgatttcg aacagaagtt caaggcgctg
     7321 gccaagtctc agggccgcta ctcgaacact ctcgaactta atctcaagct gaacgattcg
     7381 gtcggtacta aggccaagat caacgtgatc aacgatcagg acgaacgcga tatcgtcggt
     7441 acgacggaat acgagcgcaa cgatcgtacc ggttactttg aggtcaactc caatatcaat
     7501 ccgcgtgtct ttgccaaagg aagcgtggaa caccaggtgc gctgggtgaa cgcgccgaag
     7561 gaagacattc gcatcgatta catcggagaa accacctaca acttcacgcc gacactcagc
     7621 ttcacagccg gggctcagca cgtcgacttc gaaagcctcg gcgaacccgg caaatcgagt
     7681 ctggcgaatg cgattctcgg cgagttgaaa aagaacttca ccgacaagtt ccgtggccgg
     7741 gcgttctata cccgcgcggt tgtcgactac aaggatggcg agaccgacac catcgatcgc
     7801 gaaaacatct acggcgaact catctatgac atcagcaagg atgccagctt caagctgaag
     7861 ttcggctacg actatccgaa cgacggtcgc tgggacatca gcagtgtcgg caacggccgc
     7921 gacttcaagg gcatcgaaac ccagaagatg atgatcttcg aagccaagac caacttctag
     7981 gaccactggc catgaaacgc aaactcctcc tgctcctggc actttccgtt gcgatgctgg
     8041 cgactggctg cattcaggaa aagctgttca ttccggttgc gaccgtgacc ggcaagatcg
     8101 tcgttccggc cggaaaagat ccgattggta tcaagatcac tgtcgccggc aacccctcga
     8161 tcacgaccta tgtcaatgaa accggtgatt acaagctgga gtttccgaag accggccggt
     8221 acttactggt tgcccgcagt aaatactatg atgtcgatta cgtctgggtc gacgcgtttc
     8281 tcgaacagac cgtcaaggcc gacaacctgt cgctgaaaga aaagattgtc ggcgaagtca
     8341 agttcctggc taccatcgtc gactatccga atgccgaatg gttcaaggtc aagagcatca
     8401 cccccgtctg ggcgaccgac acggtttcgc tgtatgacga tggaacgcat gatgaccgtc
     8461 tggccaacga tggcatcttc acgctgcgct tgaccaatct cgccactggg tatcaacagt
     8521 accacctgat ctggtatggc gataaggatg gcgagatcgg tgagaatacc gagaaactcg
     8581 atccgcatcg cgaagccgag cgcgtaggca actccgaaat gtggatccgc gaacctgagg
     8641 tgaagctggc ccgcggtcgc attacctcgg cgctcaccgg ggtgaactac tctgaagtca
     8701 ccctggcgac aaagggcggt gcccgcaaga tgctggtcaa cagtgatggc acctacagca
     8761 ttgcgatgga aggcaatggt cgtgagtatc tggtgttccg cagtgtcaac tttcatgtgc
     8821 gggcgattcc cgtcgatctg acgaccgtac cgatttacga tgtgccagat acagtgttga
     8881 aggcaaaagc gccgggtgag atcaagctgg tgttggtgaa gaacgatttc caggatgtgt
     8941 tgaaccccat ggtggtcggc gaattcaccg actggcatcc tgaagccatg tatgatgatg
     9001 gcacgcatgg tgacgaagtc tctggcgatg gcgtctatac ctacaccaag agcggcgtcg
     9061 gccctggtta ctacaagtat gcctacaaca ttcgcgaagg caatcaggtg cgcgatccgt
     9121 atgaagaatc aggtgattcg tccttctcga tcctgacggt gaaatagaga tgatgcgaag
     9181 attcacagcg cttgttctga tcctgctggt tttcgtgacc gtgcaggccg aagcgcgcga
     9241 gtatgcgcca ccgaccaact acttctacga ggccgggtcg gtgttcgatg tcagcgaccc
     9301 cgtcggtgat gacaaaggcc cgggctacta cacctacccg cttgactcgc gtctgcgccg
     9361 cggcaccttt gatgtcacgc gtttctacgc ctatgaagaa ggaaaagtat tgaccttcgt
     9421 cattcagttt cgcaactacg tgatgaccga atggccgaat ggcaaaggca gtgaagacca
     9481 ggggttcgtc gccaatctgt gcgacatcta catcgacatg aaccgccaac cggggcgcgg
     9541 atacaacaaa gcgctgcctg ggcgacagat cgagtttgca gatcagatgg gctgggaaaa
     9601 gatgattctg gtgactccgt tgtctcagtg gcgggtctac gagcagctca aggacaagac
     9661 t
//
LOCUS       DAORHD010000016         9499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6597_length_9499_cov_3.659043, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000016 DAORHD010000000
VERSION     DAORHD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_9499_cov_3.659043"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PKO06_00780"
     CDS             complement(<1..1008)
                     /locus_tag="PKO06_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNV68200.1"
                     /translation="MKRKPLFWFLFLFVEICACSLVAEVHVRSAGVTDVGAPGEVSVL
                     SPFERQYLASWDRISGAFLAKTLRTDAGDLFAPGSNLVFSVRSGINGYLTILSIDERG
                     NMVVLFPNELEPTAKITGGTERIIPAPTAKYAFKLGKGPAYDLVKVILSVEPNQVIDT
                     LARSPLKPYERIDSPRQAVQTFQELLKRSNTLHAVSTIAVHTASATPDSFAVGLATLT
                     TRIGTAFLIKGWTDPLHIRPNQPFRLLLHPNAPCRLIRIDSISSPGHEKQLMAASFDL
                     TTGVCTYPPVESDLVLVADGDSEKETLRLLFDDNYSGKQATMTLVIPVLPQATAAADP
                     QV"
     gene            1200..2594
                     /locus_tag="PKO06_00785"
     CDS             1200..2594
                     /locus_tag="PKO06_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68201.1"
                     /translation="MASISRKNALRTLWLALVLTLVQAAVFAAALNVVYERVITDSWG
                     NILGTELQTIRSVNDLPYGSPWRSYLLMQTGGRDLLSYSSEVARYLNQPLNITISDRN
                     SVSNSSKSSDGYHITLFQHARSYSSEDSKKFIFLHEMGHVAMLNSYPGSYNFSGLNYG
                     TDGVHYMDEILPNHKTAWVEGWANAFAALKNNGKVFSLDMKSDSIVAFLKVNTFEQMT
                     RNELFIGKLLYDISRTFPSGTDKVYDVLARTGPHNSLRDFTRAYLSVYPGDQIGLARL
                     LDKLSWGKISQAELLEYVNNGSSTVSYDFYQYLQSRSGTVASNNSTGSTKKGFWARVG
                     DFFSNLFAGLFGRKDSAQTAASSAGVNTGSNALTPSGVSSQGTAALAPVVTGSPAAAS
                     SGSVSSALTPLSPEGIAQAQEDYFKAFAAYNNVLAKSDINSPEVKQALAVLQAAKQRL
                     ETIRQSIPQDATSK"
     gene            2791..3672
                     /locus_tag="PKO06_00790"
     CDS             2791..3672
                     /locus_tag="PKO06_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNV68202.1"
                     /translation="MPPLQLAAFLLFTIVAFSSIEVVSHPIRDQIDPMLLTFWRFHLG
                     GLCLLILGSAFGDFRRHVPPQRSDWLQLTLLGALNVILSMGALAVAIKYASASTVAIL
                     ISSNPLATNVFARLLLAEPLDTRRWIALLCGVTGVVLICLRPTGTIDTAFGIGCGLAA
                     MLTFGLYTVVSKPLVARLGSLPVAAYSFGLACLIYFPFLWVHLPTVWPANELWGRILY
                     LGIVVSGLAYVTFYRALAVLPAGRASLLFFCKPPTAGLLAWLVLGETPGQTVLIGAGL
                     VMFGLLIDRRPSSESRR"
     gene            3835..4626
                     /locus_tag="PKO06_00795"
     CDS             3835..4626
                     /locus_tag="PKO06_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV68203.1"
                     /translation="MERSTIIGFFGTWVLLLIGIKLAGPIVAFIDYPSIMIVLGCTTV
                     SCLVAYPFQEVKKTFGMMLSCFKESEKFDYAKSITDILAISEVARKEGLLSLDTKLNE
                     IQDPFMVRILRMVVDSIDNNIISDVIQTEIDSRGSRHEDTKTIIEFMASVGPAYGMVG
                     TLIGLVLMLRDLNDPSTIGPNMAVALVTTFYGSLLANLCLTPFIKKLVARSQAELLHL
                     EIIGKGCQMIAAGVHPRIISERLVSYLPTKERAKVADIDLERKTS"
     gene            4626..5432
                     /locus_tag="PKO06_00800"
     CDS             4626..5432
                     /locus_tag="PKO06_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNV68204.1"
                     /translation="MAKRKSAPGPGAPEWMLTYGDMVTLLLCFFVLLFAYSTTDIVKI
                     KAVQQSMNDAFSFSFSINNQSGSAQRVVPGGKGFVNMPNAANMMPIPKIVQRAKKLLG
                     KVSIRDNLEIVAGKQDVRLRVPSGVMFERGFAVLKPQAKELLDALSPLLTEVPDNDIQ
                     IDGHTDDAPLSNPQFPSNWELSTARASAVARHFIEEHEINPKRFCVRGFASFQPLYPN
                     DTDENRAKNRRVEIVLVTPLTPFKQDKPKSGLWENPNLRDPELDKPSPNW"
     gene            complement(5503..5661)
                     /locus_tag="PKO06_00805"
     CDS             complement(5503..5661)
                     /locus_tag="PKO06_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68205.1"
                     /translation="MIWFFLFTGLGLTIALFLLITRELLQVSARRIVGGALLVSVLTA
                     LIRVSQIT"
     gene            complement(5658..6668)
                     /locus_tag="PKO06_00810"
     CDS             complement(5658..6668)
                     /locus_tag="PKO06_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68206.1"
                     /translation="MTRLLLLCALILLISPASAVTPDIRLPEWHQLDLKLKPAEKTDS
                     LKLCVSVRALIADLRDLSISMRLPNGMTVTPAEQRQPFLKRGETWEATFVTGFKPGAN
                     GWVEIAAAARPDIPQMEQWLRNVATYSATARLILQTEIQRFIEPMQIGRIFPISVTTE
                     LSALTPRFFLFESLPLGKAHRVFVWAPDGEFSLPAPKRALREMRALMQREEWGKAAAS
                     ARALATAVQSAAEITFIAPGQRRMSLSKDMINSAIAASVPALEALAGNHEEAHQALQR
                     QATEDKNPFSAYIQANLGALHATNGRVSEARAAFTKALDLFPLWILMREQLARLSSGG
                     EK"
     gene            complement(6695..8242)
                     /gene="lysS"
                     /locus_tag="PKO06_00815"
     CDS             complement(6695..8242)
                     /gene="lysS"
                     /locus_tag="PKO06_00815"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNV68207.1"
                     /translation="MEELNQVQRQRLDVIEELRREGHEPFGRGFERTHTNAKAKEAFC
                     QAEAAAEQKEHFEFGPVTVVGRIIAKREQGKAAFAHIMDQNDKIQVYLRQDVLGEKAF
                     AIVKRLYAGDIVGIEGPMFRTRTNEVTVKAQKLTILSKSINPLPEKFHGLTDVELRYR
                     QRYVDLIANPEVRETFIKRSRIVQLVREFMNTKGFLEVETPMMHPIPGGAAARPFITH
                     HNALDMQLYLRIAPELYLKRLLVGGFEKVYEINRNFRNEGISIKHNPEFTMMEVYQAY
                     GDMEVMMNLTEELVTSVAAEISPDLKVPFQEKTIDLNRPWKRVKMIDAVREVAQAPEL
                     DFNSPRDLAAGKAKALGIHVDPKESTAAIIVKIFEEKIESTLINPTFIYDYPKEISPL
                     AKASAHDPTRVDRFELFMNGWEMANAFSELNDPEEQKLRFEEQLTQREKGDEEAHCMD
                     EDYVNALRYGMPPAGGLGVGIDRLVMILTNSPSIRDVILFPTMRPRVQGSSTATEGAV
                     TSEGKPS"
     gene            complement(8337..8537)
                     /locus_tag="PKO06_00820"
     CDS             complement(8337..8537)
                     /locus_tag="PKO06_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNV68208.1"
                     /translation="MQSRVKWFNNAKGFGFIENTTGEKDIFVHYKQIAGDGYRSLEQG
                     QTVEFEIEMGPKGPQAVNVRKI"
     gene            complement(8679..9167)
                     /locus_tag="PKO06_00825"
     CDS             complement(8679..9167)
                     /locus_tag="PKO06_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNV68209.1"
                     /translation="MTTILGLDLAQKTGLCVLAAEGPRLIWSDSVLLAAKDPGKRLLA
                     LQKVLVDIIERYHPDEIAMEDVFLPAKTSPRTPISLGELRGVARLCAARFEIPVSFYA
                     PRQVKMTITGNGAAAKDDVIRLVESEFHVHLKDDNQADAISVAYTHMLARRFRNFEGV
                     KV"
     gene            complement(9164..9334)
                     /locus_tag="PKO06_00830"
     CDS             complement(9164..9334)
                     /locus_tag="PKO06_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68210.1"
                     /translation="MKDYEWKRALFIGGVMFPILVIAQYYGHDSMWIPAVTAGVSSGI
                     SVILFPNPDHKP"
BASE COUNT         1968 a         2574 c         2734 g         2223 t
ORIGIN      
        1 aacctgtgga tcagctgctg ccgtggcttg cggtagaacg ggaatgacca gagtcatggt
       61 ggcctgtttc ccggaataat tgtcatcgaa aagcagtcgc agggtttctt tctcggaatc
      121 tccatcggcg accaggacca gatcggattc caccggagga tacgtacaga ccccggtggt
      181 gaggtcgaat gacgctgcca tgagctgctt ctcgtgccca ggcgaactga tgctgtcgat
      241 tcggatcaga cgacacggtg catttgggtg gagcagtagg cgaaagggct ggttggggcg
      301 aatgtgcagg ggatcggtcc accccttgat gagaaaggcg gtgccgatac gagtggtgag
      361 ggtcgccagc ccgacggcaa aggagtctgg tgtggcactc gccgtatgaa ccgcaatcgt
      421 tgacacggcg tgaagcgtat tcgaacgctt gaggagttcc tgaaaggtct gtacggcctg
      481 tctgggagag tcgatgcgtt cgtatggttt gagcgggctg cgagcgaggg tgtcgattac
      541 ttgattcggc tctacggaga gtatgacttt gaccagatca tacgccgggc ccttgcccaa
      601 tttgaaggca tacttggcag tgggggcggg aatgatgcgc tcggtgcctc cggtgatttt
      661 cgccgttggc tcgagttcgt tcgggaacag cacgaccatg ttgccccgtt catcgatgct
      721 caggatggtg agatacccgt tgatgccgct gcgtaccgaa aaaacgaggt tactgcccgg
      781 agcaaacagg tcaccggcat cggtgcgaag cgttttggcg aggaatgctc ctgaaatgcg
      841 atcccaggac gcgagatact ggcgttcaaa tggcgagagc accgaaactt caccgggcgc
      901 gccaacatca gtaacgccag ctgatcgaac gtgaacttca gccacaagcg agcaggcaca
      961 gatctccacg aagagaaaga gaaaccagaa aaggggcttc cgtttcatgg ccgtcctcca
     1021 ttgatccata ttgtgtgtgg tagtgtatcg cacgtcaggg cgcaagtgca aaactgcgcc
     1081 tgtactcaga tttggtgtcg atatgacgaa aaacttgtca tttcggtacc ccagcttctg
     1141 gacggttcgc ggaacgatca ctataatgaa tctaatcatc gcgagtaagg gagcgggata
     1201 tggcgtcaat ttctcgaaag aacgctctgc gaacactgtg gctggctctg gttctgactc
     1261 tcgtacaggc ggccgtgttc gcagccgcgc tgaatgtcgt gtacgaacgg gtcatcacgg
     1321 atagttgggg caacatcctt ggcactgaac tgcagacgat ccgttcggtg aacgatctcc
     1381 cttacggcag tccatggcga agttatcttc tcatgcaaac gggtggacgt gatctcctca
     1441 gctattcctc cgaggtcgcg agatatctca atcaacccct gaacatcacg atcagtgatc
     1501 gcaactccgt ctcgaattcc tcgaagagct cagacgggta tcacatcacg ctgttccagc
     1561 acgcgcgctc ctactcaagc gaagactcca agaagttcat ctttctgcac gagatgggcc
     1621 atgtggccat gctcaattcc tatcctggat cgtacaactt ctcaggactg aattatggca
     1681 ctgatggtgt ccattacatg gatgagattc tgccgaacca caagaccgcg tgggtcgaag
     1741 gctgggccaa cgcatttgcg gccctcaaga ataacggcaa ggtgttctcg ctcgacatga
     1801 agtccgattc gatcgtcgcc tttctcaaag tgaacacgtt tgagcagatg acgcgcaatg
     1861 aattgttcat cggaaaactg ctatatgata tctctcgcac gttcccttcg ggaacggata
     1921 aagtctatga cgtgctggca cgaacagggc cgcacaacag cctgcgcgac ttcacccgcg
     1981 cctatctcag tgtctatccc ggtgatcaga tcggtctggc gcggttgctc gacaaactga
     2041 gctggggaaa aatctctcag gcagagttgc tcgaatacgt gaacaacggg agtagcactg
     2101 tcagctacga tttctatcag tatctgcagt cccgctcggg aaccgtggcc tcgaacaact
     2161 caaccggctc cacgaagaag gggttctggg ctcgcgtggg cgacttcttc tccaacctgt
     2221 tcgccggctt gttcggtcgc aaggattccg ctcagacggc ggcaagttcg gccggcgtca
     2281 acacagggtc gaatgctctg acaccgagtg gagtttcgtc tcaaggcacg gcagcgctgg
     2341 ctcctgtcgt aacaggttcg cctgcagccg cgtcatctgg cagtgtgtcg agtgcgctga
     2401 ctccgttgtc gcctgaaggc attgcccagg cccaggaaga ctacttcaag gcatttgccg
     2461 cgtataacaa tgtcttggcc aagtctgaca tcaattcgcc tgaagtgaag caggctctcg
     2521 cggttctgca ggcggcgaag cagcgactcg aaaccattcg ccagagcatt ccccaggacg
     2581 caacgtcgaa gtagtcgttc gccccccccc ggccttccgg cgcttctgct gcggaaggcc
     2641 ggttctcttt tcaggggctg tgtgcctcct gtcggggttg aaagttcatg tgtcagaggg
     2701 accatcgtct cgacaattct gccagaactc gcatagctgg gcattcacca ttgtgaatgg
     2761 cctgtaatcg tggtagaacg gcgtcaccct atgccgcccc tgcaactcgc cgcctttctg
     2821 ctgttcacga ttgtggcctt ctcctccatc gaggtggttt ctcacccgat tcgcgaccag
     2881 attgacccga tgttgctgac cttttggcgc tttcatctcg gcggactttg tctgctcatt
     2941 cttggaagcg cgtttggtga ctttcgtcgg catgttccgc cgcagcggag tgattggttg
     3001 cagttgacgc tgttgggcgc actgaatgtt atcctcagca tgggagcgct tgctgttgcc
     3061 atcaagtatg cgtcagcatc tacggtcgcc attctcattt cgagtaaccc gctggcaacc
     3121 aatgtgttcg cccgcctcct cctggcggag cctctcgata cccgtcgctg gatcgccttg
     3181 ctgtgcggcg tgaccggtgt ggtgctgatc tgtctgaggc cgacgggaac catcgatacc
     3241 gcctttggca tcggatgcgg ccttgcggcg atgcttacct tcggactgta tacggtcgtc
     3301 tcaaaaccgc tggtggcgcg cctcgggagc cttcccgtcg cggcgtacag cttcggtctt
     3361 gcctgtctga tctacttccc cttcctttgg gtgcatctgc cgaccgtctg gccggcaaac
     3421 gagctctggg ggcgtatttt gtatctgggc atcgttgttt ctggtcttgc ctatgtcaca
     3481 ttctatcgag ctcttgccgt gcttccggct ggccgggcga gcctgctgtt cttctgcaag
     3541 ccgccgactg ccggcctttt ggcctggttg gttctcggag agactccagg gcagacggtt
     3601 ctcatcggtg ccggtctggt gatgttcggc ctgctgatcg atcgccgacc gagttccgag
     3661 tcgagacgct gaaacccacc acgacggttt acctgagagc gattgctggt gctgcgccac
     3721 cagtgctgag cctcagcctg tttttccctg attcgactca aattacgggg aacatgtgcc
     3781 gatgaccata gggggtatcc cctactcaaa tcatcgtaaa caggatgaaa caccgtggaa
     3841 agatccacaa ttatcggttt cttcggcacc tgggttctgc tgcttatcgg catcaagttg
     3901 gctggtccca ttgtcgcctt catcgactat ccgtccatta tgattgtgct tgggtgtaca
     3961 acggtctcgt gcttggtggc ttatcccttt caggaagtga agaagacgtt cggcatgatg
     4021 ctttcctgtt tcaaggaaag tgaaaaattc gattacgcca aatcgatcac cgatattctc
     4081 gccatttccg aagtggcgcg caaagagggc ttgctgtcgc ttgacacgaa gttgaacgaa
     4141 attcaggacc cattcatggt gcgcattctg cgcatggtgg tcgattcgat cgataacaac
     4201 atcatttccg atgtcataca gacggaaatc gactcccggg gatctcgaca tgaagacacc
     4261 aagacgatca tcgagtttat ggcgtccgtt ggtccggcat atggcatggt tggaactctg
     4321 atcgggcttg ttctgatgct tcgcgatctg aatgatcctt cgaccattgg tccgaacatg
     4381 gccgtcgctc tcgttacgac gttctatgga agcttgttgg cgaatctgtg cttgaccccg
     4441 tttatcaaga aactggtggc acgaagccag gccgaactgt tacaccttga aatcatcggc
     4501 aaaggctgtc agatgatcgc tgccggggtt catccgcgca tcatctctga gcgtctggtg
     4561 agctatctgc ctaccaagga acgtgcgaag gttgctgata ttgatttgga gcggaagaca
     4621 agctgatggc aaagcgtaaa tcagctcctg gccctggcgc gccagaatgg atgttgacct
     4681 atggagatat ggtcacgctt ctcctgtgtt tcttcgtgct gctgtttgcc tattccacga
     4741 cggatatcgt caagatcaaa gcggttcagc agtcgatgaa cgatgccttc agcttttcgt
     4801 tttcgatcaa taatcagagt ggttcagccc agcgagtcgt tcccggtggg aaaggcttcg
     4861 tgaacatgcc gaatgcggca aacatgatgc cgattcccaa gatcgtgcag cgggccaaga
     4921 agctgctcgg aaaggtttcg attcgagata acctggaaat cgttgccgga aagcaggatg
     4981 tgcgccttcg cgttccttca ggagtcatgt ttgaacgggg atttgcggtg ctgaagcccc
     5041 aggccaaaga gctgctcgat gccctctctc cgcttctcac cgaagttccg gacaacgaca
     5101 ttcagattga tggccacacg gacgatgcgc cgctgtcgaa tccgcagttc ccttcgaact
     5161 gggaactgag taccgcgcgt gcttcagccg tggcccggca ttttatcgag gagcacgaga
     5221 tcaaccccaa gcgtttttgc gtgcggggtt ttgcttcgtt tcagccgctg tatccgaatg
     5281 ataccgatga gaatcgagcc aagaatcggc gggtcgaaat tgttctggtg acgcctctga
     5341 caccgttcaa gcaggacaaa cccaagagtg gactctggga aaaccccaac ctgcgtgatc
     5401 cggagctcga caagccctcg cccaactggt agagcgaaaa cctcgcacca aaaaagaagc
     5461 gggtcggcaa ggcaccgacc cgcttttcca tttccagtgg aactacgtga tctgactgac
     5521 tctgatcaat gctgtcagaa ccgacaccag aagcgcgcct ccgacgatac gtcgggccga
     5581 cacctgcagc aattcgcgtg tgatcagcag aaacagggca atggtaagtc cgaggccggt
     5641 gaacagaaaa aaccagatca tttctcacct cctgacgaaa gccgtgccag ctgttcacgc
     5701 atcagtatcc agaggggaaa cagatcgagt gccttggtga aggcagctcg ggcttcgctg
     5761 acgcgaccat tggtggcgtg caacgcaccc aggttggctt gtatgtaggc actgaaagga
     5821 tttttgtctt cagtcgcctg tctctgcaag gcctggtgcg cttcttcgtg gtttccggcc
     5881 agggcttcaa gggctgggac agaggccgcg attgcagaat tgatcatgtc cttcgagaga
     5941 ctcatgcgtc gctgcccggg tgcgatgaac gtgatctcgg ctgctgattg caccgccgtc
     6001 gctaaggcac gtgccgaagc ggccgctttg ccccactcct cgcgctgcat cagagcccgc
     6061 atttcacgca atgcgcgctt gggagcggga agtgagaact ctccatcggg agcccagaca
     6121 aagacccgat gtgcttttcc gagaggcagg ctctcgaaca gaaagaaacg cggcgtgagg
     6181 gcggacaact ccgtcgtgac actgattgga aagatgcgtc cgatctgcat cggctcgatg
     6241 aaacgctgaa tctcggtctg caggatcaat cgcgcggtgg ctgaataggt ggccacgttc
     6301 cgcaaccact gttccatctg cggaatgtcc gggcgggcgg ccgcggcgat ctcgacccag
     6361 ccgttggctc cgggtttaaa gccggtgaca aacgtcgctt cccaggtttc gccccgtttc
     6421 agaaagggct gccgctgctc agcgggggtg acggtcatgc cgttgggcag gcgcattgat
     6481 atcgaaagat cgcgaagatc agcgatcaaa gcccgcactg agacacacag tttcaaggag
     6541 tctgttttct ctgcgggctt cagcttcaga tcgagctggt gccattcggg cagacgaatg
     6601 tcgggcgtga cggcagacgc tgggctgatc agcagaatca gagcacagag caggagcaga
     6661 cgggtcatga ctcaatcctt tgtgaggcgg tggctcagga aggctttccc tctgatgtta
     6721 ccgcaccttc ggtcgcggtg gaagatccct gtacacgcgg tcgcatggtg ggaaagagaa
     6781 tgacatcgcg aatcgaggga gagttggtca gaatcatgac gaggcgatcg ataccgacgc
     6841 ccagtccacc ggcgggaggc atgccgtagc gcagcgcatt gacatagtct tcatccatgc
     6901 agtgcgcctc ttcatcgccc ttctcgcgct gtgtgagctg ttcctcgaac cgcagtttct
     6961 gctcttcagg atcattcagc tcagaaaacg cgtttgccat ttcccaaccg ttcatgaaca
     7021 actcgaagcg atcgactcga gtgggatcgt gggcggaagc tttcgccaga ggggaaattt
     7081 cttttggata atcgtagatg aaggtgggat tgatcaaggt tgactcaatc ttctcttcaa
     7141 agattttcac gatgatggcg gccgtgcttt ctttcggatc gacatgaatg cccagagctt
     7201 tggccttgcc ggcagcgaga tcgcgagggc tgttgaaatc gagttcgggg gcctgcgcca
     7261 cttcacgcac ggcatcgatc atcttgaccc gcttccaggg ccggttgaga tcgatggttt
     7321 tttcctggaa tggaaccttc aggtcagggg agatttctgc ggcaacgctt gtcaccagct
     7381 cttcggtcag attcatcatg acttccatgt cgccgtaggc ctgatacact tccatcattg
     7441 tgaactcggg attgtgcttg atcgagattc cttcgttgcg gaaattgcga ttgatctcgt
     7501 agactttctc gaaaccgccg accagcagcc gcttcagata gagctctggt gcgatgcgca
     7561 gatacagctg catatcgagg gcattgtgat gggtaatgaa cggccgggcc gctgcgcccc
     7621 cgggaatcgg gtgcatcatg ggggtttcga cttcgagaaa gcccttggtg ttcatgaatt
     7681 cacgaaccag ttgcacgatg cggctgcgtt tgatgaaggt ttcgcgcact tccgggttgg
     7741 cgatgaggtc gacgtaacgc tgtcgatacc gcaattccac gtcggtcagg ccgtgaaact
     7801 tctcgggaag tggattgatg gacttggaca ggatggtcag cttctgcgct ttcaccgtga
     7861 cttcgttggt gcgggtccgg aacatcggac cttcgatgcc gacgatatca ccggcataca
     7921 gacgcttgac gatggcaaaa gccttctcgc ccaggacatc ctgacgcaga tagacctgga
     7981 tcttgtcgtt ctgatccatg atgtgggcga acgccgcctt gccctgctcg cgcttggcaa
     8041 tgatgcggcc gaccacggtg accggcccga attcgaaatg ctctttctgc tcggctgcgg
     8101 cctcggcctg acaaaaggct tctttggctt tcgcattcgt gtgggttcgc tcgaatcccc
     8161 tgccaaaggg ctcgtgcccc tcgcggcgca gttcttcgat gacatcaagg cgttgacgct
     8221 gcacttggtt cagttcttcc atgtaattct tccttcttgg ctttgaaaag cttcagcaat
     8281 aagaaagggg cgaatcggct gtggccgtcg cccccttcag tgaaacaaat gtatggtcag
     8341 atcttgcgga cgttaacggc ctggggacct ttgggaccca tctcgatctc gaattcgacg
     8401 gtttgcccct gctcgagaga gcggtaccca tcgccggcaa tctgtttgta gtggacgaaa
     8461 atgtcctttt caccggtggt gttctcgatg aaaccgaaac ccttggcatt gttgaaccac
     8521 ttgacacgac tttgcattgc tacgataact ccttgcctga ttgatcgagg gacgatattg
     8581 aaacgaggac tggatgcact tctggcgagg tctgcccgaa gtgaaaccgg atattatcac
     8641 agtggaccat ggaatgcaac tgaaaaaacc gcgaataatc agactttgac gccctcgaag
     8701 tttcgaaaac gccgcgccag catatgggtg taggcgacgc tgatcgcgtc agcctgattg
     8761 tcgtctttca gatgcacgtg aaactccgac tcgacgagac gtatcacgtc gtcttttgcg
     8821 gcggctccgt tgcccgtgat ggtcattttc acctgccgtg gggcatagaa ggaaaccgga
     8881 atctcgaatc gggcagcgca cagacgagcg accccacgga gttcgcccag ggagatcggg
     8941 gttcttggcg aggttttggc gggcaaaaac acgtcctcca tggcgatctc gtcggggtga
     9001 tatcgttcga tgatatccac cagcactttc tggagagcca gcaatcgctt cccaggatct
     9061 ttggctgcca ggaggacgct gtcgctccag atcaggcgtg gaccttcagc tgccagaacg
     9121 cacagaccgg ttttctgggc cagatcaagg cccaggatcg tcgtcacggt ttgtggtccg
     9181 ggttgggaaa gaggatgacg gagattcccg aggagacgcc ggcggtcaca gccggaatcc
     9241 acatggaatc gtggccataa tactgcgcaa tgacgagaat cgggaacatg actccgccaa
     9301 tgaacagggc cctcttccac tcgtagtctt tcatcgccga ttcccgtcaa agggcatgcc
     9361 acgtcctgcc ggtcgcgggc gattgccgcc gcccgagcgt cgtcgtcgtt tctgattgcg
     9421 gggtgggcga gccagtcggt tttcgcgcat ttccagcgtt cgccgtgtcg gcgtagcttc
     9481 ctcttcgatg ggctcatcg
//
LOCUS       DAORHD010000017         9326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6800_length_9326_cov_3.744903, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000017 DAORHD010000000
VERSION     DAORHD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9326
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6800_length_9326_cov_3.744903"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKO06_00835"
     CDS             complement(<1..369)
                     /locus_tag="PKO06_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV68211.1"
                     /translation="MQYRFPTSFVWGAATAALQIEGATAEDGRGPSLWDTFCHQHPER
                     IFEHANPDIACDHYHRYAEDVELIKAMGHTGYRLSISWSRLFPTGDPTQLNQAGVVFY
                     RRLLDALSAKQIVANVTLYHW"
     gene            556..1128
                     /locus_tag="PKO06_00840"
     CDS             556..1128
                     /locus_tag="PKO06_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68212.1"
                     /translation="MQQQQQHPRGRAGDRSLQTRGMSLIELLIAMTIFGLILAGCFQL
                     FRNFSGRNAVRLTARLELQLEVRRAMVNLYREIQEGIEIFKPDPGATLPFLMLRDYLN
                     NLHLLYLEKDLAATKREGCDMFRLYSVTYDVGTQASSAPREVLANITSMNFTSHGFAG
                     VLITGYLRREQASFSFVNMIRLKNATAGDT"
     gene            1128..2237
                     /locus_tag="PKO06_00845"
     CDS             1128..2237
                     /locus_tag="PKO06_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68213.1"
                     /translation="MRCVSRIPAERLGIALGVAVAILAIMFILAFSYFTLMRGVGSGL
                     EGADAHVRALAIAEAGTQAITARVFSGSWEGRWFRNGPDIQDKPISYGGGSYISVIQD
                     TPGVPLSFDLWVRSTYRGTRRLLFYRISYEDSVFHGLVNPAIDFTATFAEGTGSASLT
                     GGDLDPLTQKVNDLIGQRRQTSGNLGEARQRLAVKIDPLAILREVGAAAPKNGVLAVA
                     SAPVGISTVVPPPPVVPPPVGGQSTVKNVDRERIFNWLEKRGYDSDYLAALKGRANAQ
                     FAKIEQATRALKFLEAEIILRNLMQYLYGRTGAVNTQVNLEVARYRDAVQALETKAAA
                     EGWTVEQLAAAKRELELEHRARRQQIYQTAWSETP"
     gene            2234..3694
                     /locus_tag="PKO06_00850"
     CDS             2234..3694
                     /locus_tag="PKO06_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV68214.1"
                     /translation="MRHQTRRAITLVEILIALGLLAFSMVVLITTFQGAGREMAFTAE
                     HYSAMFLAQKVLEDVNQRLQENPHTFTELVQDATGQEVPVVGGASKYFRLLENTVRFD
                     RLLPDEDQSIVAGPLFEQLKGFTVKVSTRLDHPLAAGQRNLLELEIVIGWKGQDGHQK
                     EYRLSQLLQGTDDAVFAEPYSPTVPTPETQARLDRGALKALADMLAPMHPQLAAIPED
                     QLKVQAILQVCPSASQQAVMLFGQVLFLIDDCLSVVDRLNATIRPLEMERDQLQPLLT
                     KVGKTSGDFAACLKFTDLQRRISSLYQQKGVGVVQRVLLLIPLLPQLKGMLMDPAASL
                     GPRLSLYTPTLVGKLLMTNQAVDIVQLAFSSAEKCYLSLTVSPILESLPRRQEPEVLR
                     RVLDLQKIVTLMLPGDGDAEKNCKGYQQNLRQFREKYLGHHPNFVAFLDRESERGRDL
                     RTLRQNFTGLVQVFEVVRSLSDHVDDVVSSVPKAFR"
     gene            complement(3745..5601)
                     /locus_tag="PKO06_00855"
     CDS             complement(3745..5601)
                     /locus_tag="PKO06_00855"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68215.1"
                     /translation="MRTLKAAAGAVALAVVATALLLVISAGGVFSTWALDWFWRLNGP
                     LKKPTGGISGIGDQVVIVAIDEPTLRRYRGERPRLPRDVYARIITQASQMGARLIGFD
                     VTFDEPGTAEEDRQLAEAARTSGTVVTNCYLANNENFSRIWISGRAFFREHAKAEGFA
                     DFPLDLDRDKFIRRVRLYYPRGDLPCRVSFAIALYLADIGQTPDSVLYERTALVLPRP
                     GGLDSIRIPLDHRGLSLVGFLGGAKSLPTFSATDLLDGKIPPEALRDRIVLVGGTAAE
                     YRDSFHTPFSPLGDLPGVELHGHLLENLFSGILLREVSGPTWWAILLLTASVASFFVH
                     RVGTIAGGIVLSLVGVACLPLALLLFVNRMTFINPFDLWATLAVTWTSVTFYDSYRLR
                     NEKNQLAKLFRQYVSPHLLQELLEHPESIARGGARRQAVILFADVRGFTTICEESSPE
                     HVISFLNTYFNAMTKVIFRFEGVIDKFMGDGLMAFFGVPLFHDHTVVRAVEAALAMQQ
                     ALVDLQQMYRQQGEFPIRRIGIGIHGGDVVVGNVGSDLYQEYTVLGDAVNVAARLESQ
                     AMNGGILISGWIKDHLPADRFSLRSCGALKVKGRKGEVEAYEVTAITTTDVQ"
     gene            complement(5657..6316)
                     /locus_tag="PKO06_00860"
     CDS             complement(5657..6316)
                     /locus_tag="PKO06_00860"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV68216.1"
                     /translation="MRTLLHAIIGIILLSGLLLTGCGNQPSTATKDPLAFVEKIEKGE
                     AFVKRAGETTFNAASVKAQLHEGDIVKTGDTGEVVIRFATGAVARVLAKSEYQLKERK
                     VATTGQNVVYTRLVTGVAAFYVPKDAEAAKKFEVETERAVASIKGTTFKLADDGSTTT
                     LSVGEGTVNFTAKAGGKSVDVAEMYRVSANATGLSEPEKFNTLMDPDLAGAEEIMKGT
                     R"
     gene            complement(6433..7545)
                     /locus_tag="PKO06_00865"
     CDS             complement(6433..7545)
                     /locus_tag="PKO06_00865"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV68217.1"
                     /translation="MNQPLIPLVTPTERFRGLLFGTAIGDALGLPREGLEPARARRLF
                     PEPFRHRMFLGYGVTSDDTDHTLMVARALLKHPHDPAAFARALSWYLRLWILSLPPGI
                     GFATLRSIAKLWCGFGPTSSGVFSAGNGPAMRVAPIGAFFAQQPELREVYVRASTLIT
                     HTDPRALIAAQAVAEMAAWIMDKAMPHDLYPETLIWKLDGVADIKGIDPDSDAVVEWK
                     HLVTEVGRALEKNLTVTAFAQALDLGHGISGYAYHTVIMAIYAWFHHRNDFHGTIESI
                     IACGGDTDTTAAIAGALAGATTGTSGIPNPWKMYLVDWPRSIAFYWEMGSAMAEAYAG
                     QHARTPWYFTPGLLVKNLATLPILLLHVMRRLLPPY"
     gene            complement(7625..8731)
                     /locus_tag="PKO06_00870"
     CDS             complement(7625..8731)
                     /locus_tag="PKO06_00870"
                     /inference="COORDINATES: protein motif:HMM:NF023327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3419 family protein"
                     /protein_id="HNV68218.1"
                     /translation="MPITDEVVYANVWEDPELNRRSLHVKPGDSVLSITSGGCNSLCL
                     LLENPGRVVSIDLNPAQLGMLEYKRAAIMELEYDDYLEALGVTFYGKSPRHPSEYRVQ
                     LFDRIKKHMSPEARSFWEGRRERIAKGIFMDGKVEKFFAIYRRMLKWLYNWSDIERLF
                     SFGTLEEQRNYYLGMNRRKWKFLNQCLLNKFVLSLVKGSHSFEQVEDPDLAANLNRKI
                     HRGMTRFFNPDNYFMSLMLLGEHYKPDAMSPYLLRENFPVLKENIGRLEIFKGVLTDV
                     LATYGKASFDKLQLSNIFEWMTNEFFNGVIREAIDLARPHARFCWRYTLARPRTLDAT
                     NSSRLRAEPELAQKLFDLDRSFIYESFHVYQLIS"
     gene            complement(8772..>9326)
                     /locus_tag="PKO06_00875"
     CDS             complement(8772..>9326)
                     /locus_tag="PKO06_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV68219.1"
                     /translation="TDVKIVFGGYVPLYSRLIEYLTVSQVSSFGWALVTIFGTMAFLL
                     KRVQAMFLGIVPNLYPIIMTMGMMGFLGIRLDIATVTIASISLGIAVDDTIHELFLFY
                     HPDRQHLDPVESISDTLIEAGPAVIVTSTIYALGFSALILASIKSVILFGGLLAMTIV
                     FAMLCEITILPALVCTFKSWLKRT"
BASE COUNT         2019 a         2532 c         2809 g         1966 t
ORIGIN      
        1 ccaatggtac aaggtcacat tggcgacaat ctgcttcgca gagagggcgt cgagtaatcg
       61 tcgatagaac acgactccag cctggttgag ctgggtgggg tctccggtcg gaaagagccg
      121 ggaccacgaa atggaaagcc ggtatccggt gtggcccatg gctttgatca gttcaacgtc
      181 ttcggcataa cgatggtaat ggtcacaggc gatgtccggg ttggcgtgtt cgaaaatgcg
      241 ctcagggtgc tggtgacaga aagtgtccca gagagatggc ccgcggccgt cttctgccgt
      301 cgcaccctcg atctgcagag cggccgtggc ggccccccag acgaaactgg ttggaaagcg
      361 atactgcata ctgatgatcg ggacattcta cacgaagcga gctgacgatg caggacactt
      421 cgtcacccga tgaaaaagtt ccggtttgcc ctgctctggc aatagggcgc gagtatcacg
      481 tattccgttt cttttgttat actgttgtca cggagagaca cgaggagcgg aactttgaga
      541 gcgtgtacat accgtttgca acagcaacaa caacacccgc gcggtcgcgc gggtgacagg
      601 tcgctccaga cgcgaggcat gtcactcatc gaattgctga ttgccatgac catattcggc
      661 ctgattctcg ccggttgttt tcagctgttt cgcaacttca gcggtcgaaa tgcggttcgg
      721 ttgacggcac gtcttgagtt gcagctcgag gtgcggcgcg ccatggtgaa tctgtatcgc
      781 gagattcaag agggaatcga gatattcaaa cccgacccgg gggcgaccct gccgtttctg
      841 atgctgcgcg attatctcaa caatctgcat ctgctgtatc tcgagaaaga cctcgcggcg
      901 acgaagcgcg aaggatgcga catgttcagg ctgtactcgg tgacctatga tgtcggaacg
      961 caggcatcgt ctgcccctcg tgaggtcctg gccaacatca ccagcatgaa ctttacctcg
     1021 cacggttttg ccggtgtgct gatcaccggg tatctgcgtc gcgagcaggc cagtttttcc
     1081 ttcgtgaaca tgattcgact caagaacgcg acggcaggag atacctgatg cgttgcgtgt
     1141 ctcgaatccc ggccgaacga ttgggtattg cccttggggt cgcagtcgcg attctcgcga
     1201 ttatgttcat actcgctttc agttacttca cgctgatgcg cggggtcggt tccggactcg
     1261 aaggtgctga tgcgcacgtg cgtgccctgg cgatcgcgga agcgggtact caagccatca
     1321 cggcccgtgt gttttccggc tcatgggaag gacgctggtt tcgcaatggc ccagacatac
     1381 aggacaagcc gatttcctat ggaggtggat cgtacatttc ggtgattcag gatacgccgg
     1441 gggtgccgct ttctttcgat ctctgggttc gctcgaccta tcggggtact cgtcgcctgc
     1501 tgttttatcg catctcgtat gaggactcgg tgtttcatgg actcgtgaac ccggccatcg
     1561 atttcacggc gactttcgcc gagggaaccg gctcggcgtc tctcaccggc ggggatctcg
     1621 acccgctgac ccagaaagtg aatgacctca tcggacaacg ccggcaaacc tcgggaaatc
     1681 tgggcgaggc caggcaacga ctggcggtca agatcgaccc tctggcaatc ttgcgtgagg
     1741 tcggcgcggc agctccgaaa aacggagttc tggcagtggc atcagcaccg gtgggcattt
     1801 ccaccgtggt tccgccgccc cccgttgttc caccccccgt gggcgggcaa agcacggtca
     1861 agaacgtcga tcgtgaacgc attttcaact ggctcgaaaa gcgcgggtac gattctgact
     1921 atcttgctgc tttgaaagga cgagcgaatg cgcagtttgc caagatcgaa caggcgacac
     1981 gggctctgaa gtttctcgaa gcggagatca ttctacgcaa cctgatgcag tatctctacg
     2041 ggcgcaccgg agccgtgaat acccaggtga acctggaagt tgcgcggtat cgcgatgcgg
     2101 tgcaagcgct tgaaacgaag gcggctgccg aaggatggac ggtcgagcag ctggcggccg
     2161 ccaagcgcga gctcgaactt gagcaccggg cccggcgaca gcagatttat cagaccgcct
     2221 ggagcgagac accatgagac atcagacgcg gcgggcgatc accctggtcg agattctcat
     2281 tgcgctgggt ctgctggctt tctccatggt tgtgctgatc acgacctttc agggagcggg
     2341 tcgtgagatg gcatttaccg ctgagcacta ttcggcgatg tttctggcgc agaaagtgct
     2401 cgaggatgtc aatcaacgcc tgcaggagaa tccgcacacg ttcaccgaac ttgtccagga
     2461 tgcgacgggc caggaagtgc ctgtcgtcgg tggtgcgagt aaatactttc gtctgctgga
     2521 aaacacggtg cggttcgacc gactgcttcc tgatgaagac cagtcgattg ttgctggtcc
     2581 gttgttcgag cagttgaaag ggtttaccgt gaaagtttcg accaggcttg atcacccgct
     2641 tgccgcgggt cagcggaatc tgctcgaact ggaaatcgtg attggatgga agggccagga
     2701 tgggcaccag aaggaatacc ggctgtctca gctgctgcag ggaaccgacg atgcggtttt
     2761 tgccgaaccc tacagtccga ctgttccgac gcctgagacc caggcacgcc ttgatcgcgg
     2821 agcactcaaa gctctggcgg acatgctcgc gccgatgcat ccgcagcttg ccgccattcc
     2881 cgaagatcaa ctgaaagttc aggcaattct tcaagtctgt ccatcggcgt ctcaacaggc
     2941 tgtaatgttg tttggacaag tactctttct gattgatgac tgtctcagcg tcgtggatcg
     3001 cctgaatgcg actattcgcc cgctcgagat ggagcgcgat caattacaac cgctcctgac
     3061 caaggttgga aagacaagtg gcgactttgc cgcttgtctc aaattcaccg acctgcagcg
     3121 caggatttcg tcgctgtatc agcagaaagg ggtgggtgtc gtccagcgtg ttctgctgct
     3181 gattcctctc ttgccgcagt tgaagggaat gttgatggat ccggccgcca gtcttggtcc
     3241 gcgactttct ctctataccc cgactctcgt ggggaaactc ctcatgacca atcaggcggt
     3301 cgatattgtg cagttggctt tcagcagtgc cgagaagtgt tatctctcgc tgaccgtatc
     3361 acccattctc gaatcgttgc cgagacggca ggaaccggag gtgctgcgcc gggtcctcga
     3421 tctgcagaaa atcgtcaccc tgatgttacc cggtgacggc gatgcggaga agaactgcaa
     3481 gggataccag cagaacctgc ggcagtttcg ggaaaagtat ctcggtcatc atccgaactt
     3541 cgtggcgttt ctcgatcgtg agtccgagcg gggaagggat cttcggaccc tgcggcagaa
     3601 cttcaccggt ctggtgcagg tttttgaggt ggtcagaagt ctgtcagatc acgtcgatga
     3661 tgtcgtgtca tcggtaccca aagcatttcg ttaaaacgtt gtcgctgcgg aagttctgtc
     3721 ggagactgct cagggggcgg gagctcactg aacgtccgtc gtggtgatcg cggtaacctc
     3781 gtaggcttcg acttcacctt ttcgaccctt gactttcaac gccccgcacg accgaagcga
     3841 gaatcgatct gccggcaggt gatccttgat ccagcctgag atcagaatgc cgccgttcat
     3901 ggcttgcgac tcaagtcgag cggcgacatt cacggcgtcg ccgagaacgg tgtattcctg
     3961 atacagatca ctgccgacat ttccgacgac gacatcaccg ccatgaatgc cgatgccgat
     4021 acgtcgaatc gggaactcgc cctgctggcg atacatctgt tgcaggtcga ccagtgcctg
     4081 ctgcatggcg agcgctgcct cgacggcgcg cacaacggtg tgatcgtgga acagtggcac
     4141 cccgaagaac gccatcagac catcacccat gaatttgtcg atcacgcctt caaaacggaa
     4201 aatgaccttt gtcatcgcgt tgaaataggt gttcaagaaa gaaatgacgt gttccggact
     4261 ggactcttcg cagatggtcg tgaatccgcg cacgtccgca aacagaatga ccgcctgccg
     4321 acgagctcca cctcgagcaa tgctctcggg gtgttccaaa agctcttgaa gcaggtgggg
     4381 cgacacatac tgtcgaaaga gcttggcaag ctgattcttc tcgtttcgta gccggtacga
     4441 atcgtagaat gtgaccgatg tccaggtgac ggcgagcgtg gcccagagat cgaacggatt
     4501 gatgaaggtc atccgattga caaacagcag tagagcgagt ggcagacagg caacgccaac
     4561 gagcgagaga acgattcctc ccgcgatcgt accgactcga tggacgaaaa atgaggctac
     4621 ggaggccgtg agcagaagga tggcccacca ggtcggcccg gaaacttcgc gcaggaggat
     4681 gccggaaaaa aggttttcga gcagatgtcc gtgcagttcg actcccggca gatcgcccag
     4741 tggagaaaac ggcgtgtgaa atgagtcgcg atactcggcc gccgtgccgc cgacgagcac
     4801 gatgcggtcg cgcaaagcct cgggtgggat ttttccgtcg agcagatcgg tggcggaaaa
     4861 ggttggcagg cttttcgccc cgccgagaaa gccaacgagc gacaaccctc gatgatcgag
     4921 aggaatgcga atcgagtcga gcccgcccgg tcgtggcaga accagtgcgg ttcgctcgta
     4981 gagaacgctg tcgggtgtct gtccgatatc ggcaagatac agggcgatcg cgaacgagac
     5041 ccggcacgga aggtcaccgc gaggatagta caacctgaca cgccggataa acttgtcgcg
     5101 atcgagatcg agagggaaat cggcaaatcc ttcggctttg gcgtgctcgc gaaagaaggc
     5161 tcgacctgag atccagatgc gcgagaaatt ctcgttgttg gccagataac agttggtgac
     5221 gaccgtaccg ctggtgcgtg ccgcttcggc gagttgacga tcttcttccg ctgttcctgg
     5281 ttcatcaaac gtgacgtcga atccgatgag tcgagcgccc atctgagagg cctgggtgat
     5341 gatccgggca tacacgtcgc gtggcaggcg agggcgttcc ccgcggtatc ggcgcaaagt
     5401 cggttcgtcg atggcgacga tcaccacctg gtctccaata cccgaaatgc cgccggtcgg
     5461 ctttttcaaa ggcccattca atcgccagaa ccagtcgagc gcccaggtgg aaaacactcc
     5521 acctgcggag atcaccagca ggagagcagt ggcaacaacg gccagagcaa ccgcaccggc
     5581 ggctgccttt aacgtgcgca tgaggtgagt agtctcaacc ccggttcaga acgagcacac
     5641 tcgaacatga aacagatcag cgtgtccctt tcatgatctc ttcagcgccc gcgagatcag
     5701 gatccatgag ggtgttgaac ttttctggct cggacaagcc ggttgcattc gcgctgacgc
     5761 gatacatttc ggcgacatcc acgctcttgc cgccggcctt ggcggtaaag ttcaccgttc
     5821 cttcgcccac cgacagcgtc gtggtcgagc cgtcgtcggc gagtttgaag gtggttccct
     5881 tgatactcgc gaccgcgcgt tcggtttcga cctcgaactt cttggcggct tcagcatcct
     5941 tgggaacgta gaacgcggca actccggtga cgaggcgtgt atagacaacg ttctgaccgg
     6001 tggtcgcgac cttgcgctct ttcagctgat actcggactt cgccagcacg cgggcgactg
     6061 ctcccgtggc aaaacgaatg acgacctcac cggtgtcacc ggttttcacg atatcaccct
     6121 catggagctg agccttcacc gaagcggcgt taaaggtcgt ttccccggca cgcttcacga
     6181 aggcttcacc cttctcgatc ttttcgacga aagcgagcgg gtctttcgtg gcggtcgagg
     6241 gctggttccc gcaaccggtg agcaggagac cagagagcag aatgattccg atgatggcgt
     6301 gcagcagggt tctcatcaac agagcctcca atcacgagag ttcaaagccg tacaggttct
     6361 gatgacagga tatcagtcta cagccggatt cacaagctcc ggacaggcag aaatcgttct
     6421 gtggtccgcg tgtcagtagg ggggcagcag tcggcgcatg acatgcagca ggaggatggg
     6481 cagggtcgcc aagttcttga ccagcagtcc tggagtaaaa taccacggag tgcgagcgtg
     6541 ttgccctgca taggcttctg ccatcgctga gcccatttcc cagtaaaacg cgatggaacg
     6601 gggccagtcg accagataca ttttccaggg gttgggaatt ccggaagtgc cggtcgtcgc
     6661 cccggcgagg gctccggcaa ttgcggcggt cgtgtcggtg tcaccaccac aggcgataat
     6721 actttcaatc gttccatgaa aatcgttgcg atggtgaaac caggcgtaga ttgccatgat
     6781 cacggtgtga taggcatacc cgctgatacc gtgtccaaga tcgagggcct gggcgaaggc
     6841 tgtcacggtc agatttttct cgagggcccg tcccacttcg gtgacgaggt gcttccattc
     6901 gaccacggcg tccgagtccg gatcgatgcc cttgatatcg gcgacgccat cgagcttcca
     6961 gatcagggtt tcgggataca gatcatgcgg catggccttg tccatgatcc aggctgccat
     7021 ctcggcgacc gcctgggccg cgatgagcgc gcgcggatcg gtgtgggtga tcagggtgct
     7081 ggctcgcacg tagacttccc gaagctcggg ttgctgagcg aagaacgcgc cgatgggggc
     7141 aacccgcatc gccggaccgt tccccgccga gaacactccg gaagaggtgg gtccaaagcc
     7201 gcaccagagc ttcgcaatcg aacgaagagt cgcgaagccg attccggggg gaagactgag
     7261 aatccagagc cgcagatacc aggaaagggc tcgggcaaaa gcggctggat cgtgtggatg
     7321 tttcagtaat gcccgggcaa ccatcagcgt gtgatcggtg tcatcactgg tgaccccata
     7381 gcccaggaac atgcggtggc gaaagggctc gggaaacagc cgccgcgcgc gcgcgggttc
     7441 gagtccttcg cggggcaggc cgagcgcgtc accgattgcg gtgccaaaga gcaagcctcg
     7501 aaatcgctcg gtcggtgtga ccagtgggat cagcggttgg ttcatggtat cagactgtgc
     7561 gaatctatcc ctccatgcat ttccgatgtg aacatattcg taacgaacac tgaatgcggg
     7621 tgtgtcacga tatcagctgg tacacatgaa agctttcgta gatgaacgat cgatcgaggt
     7681 caaacagttt ctgtgccaac tcgggttcgg cgcgcagacg gctggagttc gtggcatcga
     7741 gtgtccgagg gcgtgccaga gtgtagcgcc agcagaagcg cgcgtgcggt cgtgccagat
     7801 cgatcgcttc ccggatgact ccgttgaaga attcgttcgt catccactcg aagatgttcg
     7861 acagctggag cttgtcaaac gatgccttac cataggtggc gagtacatct gtcaacacgc
     7921 ccttgaaaat ctcgagtcgt ccgatgtttt ctttcagtac cggaaagttt tcgcgcagca
     7981 gatacggcga catggcatcg ggcttgtagt gttctcccag cagcatcagt gacatgaaat
     8041 aattgtcggg gttgaagaag cgggtcatgc cgcgatgaat cttgcggttc agattcgctg
     8101 ccaggtcagg gtcttcgacc tgttcgaaag agtggctgcc cttcaccagg ctcaggacga
     8161 acttgttgag cagacactga ttcaggaact tccatttgcg gcgattcata cccaggtagt
     8221 agttgcgctg ttcttcgaga gtgccgaatg aaaacagtcg ctcgatatcc gaccagttgt
     8281 acaaccactt gagcatgcga cgatagatcg caaagaactt ctcgaccttg ccatccatga
     8341 aaatgccttt cgcgatacgt tcgcgacggc cttcccagaa agatcgtgct tcagggctca
     8401 tgtgcttctt gatgcggtcg aacagctgaa cgcgatactc gctcgggtga cgtggagact
     8461 tgccgtaaaa ggtcacgccg agcgcttcga ggtagtcgtc atactcgagt tccatgatcg
     8521 ccgcccgctt gtattcgagc ataccgagct gcgccggatt caggtcgatc gacacgacac
     8581 ggccgggatt ttcgagtagc agacagagtg agttgcaccc ccccgaagtg atcgacagta
     8641 ccgagtctcc cggtttcacg tgcagtgagc ggcgattcag ctcggggtcc tcccacacat
     8701 tggcatagac gacttcatcg gtgatcggca tatgtctttc ctccggtttg gaattggctt
     8761 gggataagaa ctcaggtgcg cttcaaccag gacttgaagg tgcagacgag tgcaggcaga
     8821 atcgtgatct cgcagagcat ggcgaagacg atcgtcatcg ccagcaggcc accgaacagg
     8881 atgaccgatt tgatactggc aagaatcagg gcggagaatc cgagggcgta gatggtcgag
     8941 gtgacgatga cggccggacc ggcttcgatc agagtgtcgc tgatcgattc gaccgggtcg
     9001 aggtgctgac gatcgggatg atagaacagg aacagctcgt gaatcgtgtc atcgacggca
     9061 atcccaagtg aaatcgaggc gatggtgacc gtggcgatgt cgagcctgat gcccaaaaac
     9121 cccatcatgc ccatggtcat gatgatggga tacaggttgg gaacgattcc cagaaacatg
     9181 gcctgcacgc gcttgagcag aaaggccatc gtgccaaaga tcgtgacgag cgcccagccg
     9241 aagctgctga cctgactgac cgtcagatac tcgatcaggc gcgaatagag tggaacatag
     9301 ccgccgaaga cgattttgac atcggt
//
LOCUS       DAORHD010000018         9311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_6816_length_9311_cov_4.389261, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000018 DAORHD010000000
VERSION     DAORHD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6816_length_9311_cov_4.389261"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKO06_00880"
     CDS             <1..903
                     /locus_tag="PKO06_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNV68220.1"
                     /translation="KILTTTSEANKLIADVWAVRADFYRDNPEIVKGLVEGIFEGMEM
                     LKSQTTKAQAFQWMADGFGMKVDDIKGMQNDAHSTNFAENRDFFLNANNATNFERTWN
                     NICFVYKEIGLMDNLVPFDQVMDFSIVKKLAEEGKFKEQQNEYVSRFVPTTYNKVQAE
                     APIITQTVRINFYPNSANIYEPKHDDIGRPMPNTLYDESIDATLEKVGRLVGAYDRAV
                     VAIVGHTDASMKGKIPEQIVKDLSMDRANSVKEALIKKFHFDPNKFVVEGKGWDQPAE
                     SSDPTNHTINRRVEVSVYSPEGGN"
     gene            986..3460
                     /gene="thrA"
                     /locus_tag="PKO06_00885"
     CDS             986..3460
                     /gene="thrA"
                     /locus_tag="PKO06_00885"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HNV68221.1"
                     /translation="MKVMKFGGTSVGDADRLRNVARLVAAAAVREPVGLVISAMTGIT
                     NHLIDSVTAVLAGKDPLSFVQVFEGRHQQALQFLQGSVSDFPLEKLQRELSAHVDEFS
                     SLLNGVRLVDECSARTLDRISCLGERALIPAMAALLQAEGLSVKVIDPRVMVLTDSVF
                     GNASPLMDEIERRFASERDLHPREVLLMPGFFGGDRRGHYTTLGRGGSDYSAAILAAA
                     LHADTLEIWTDVDGVYTADPRVVPDAFVLEEMTYTEAMELAFFGAKVLHPRTIAPVVE
                     RSIPVIIRNTFRPDHPGTRVARTTTAGKHAIRGLSSLGAVAMIGLSGAGLRGVPGVAA
                     RVFSAMAHAGISVILIAQASSEYSICFCVSEGRAPDARETLEEEFRVERQAGLVDPIE
                     VVPSLAVISAVGEEMRSRRGLAGAFFGGLATADVNVVAIAQGFSERNISAVIAGADTQ
                     RAMRAAHQSFFNTTQGIQVFLVGVGAVGRELVQQIETQQASLRQQGIDLRLCGVADSR
                     HMAINLEGYKSGAWQKALLESSTATDLRQLASIVQDARLMNPVLVDATGSDKIASAYL
                     PLLESGMHVVTPNKLANAGSWDYYLALRQTANRRRRHFLYGTNVGAGLPIIDTLRNLR
                     RSGDRLRACSGILSGSLSFLMGRLEDGVPFSQVLHEARDRGFTEPDPRQDLSGMDVAR
                     KILILAREAGLRRELAEIRLESILPAGSLTDEPVEAFLARAPDLDAHFSRLTAEAKRE
                     NKVWRFAGRIETDGSCHVGPIAVTHDHPLAAVRDGENAVSLLTDRYSPIPLVVRGYGA
                     GPAVTAAGVFADLMRTVIWHTEGVLS"
     gene            3457..4446
                     /locus_tag="PKO06_00890"
     CDS             3457..4446
                     /locus_tag="PKO06_00890"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNV68222.1"
                     /translation="MRPGGFCRAFAPATIGNLAAGFDVLGVSLAPVDSTLWGDIVTIR
                     VPRTPRRADRFTVTGPHANAVPSRTTENLVMIARRLFNQELRRRQLPTTGLELCLEKC
                     LPVCSGLGSSASSVVAALMALNTIHESPLPTPTLLELAGRAEGHVSGSIHLDNVAPSM
                     LGGIQLLVPGTNNVLQPVPLPWFSHWYLTVVHPDLQVPTALARQVLPPAFSRAAAVDY
                     WSNLAGLVHALHTGNESLAARCLRDPLIEPHRQSLVKGFDRVKREALAAGALGFSLSG
                     SGPSVFAVTESLSRAEELLDRMQQAFQRARVTSQGHVCRPDNHGARIIESDYS"
     gene            4449..5783
                     /gene="thrC"
                     /locus_tag="PKO06_00895"
     CDS             4449..5783
                     /gene="thrC"
                     /locus_tag="PKO06_00895"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV68223.1"
                     /translation="MIWYSLSQPSDTATLLEAAQRSIGHDGGLFMPRDFPRWDDVNRL
                     LTLPPRELWREIFQRLLVGELSSSEIQSLTDEAFSFPLPLTRVSEHAWSLELFHGPTL
                     AFKDFGARFLARILGHATRHQTGKPLTILTATSGDTGAAVAQAFHRVPGIRVVVLFPK
                     HRISPLQERLFATLGDNVLALRVAGSFDDCQSLVKACFRDRELCRHFGLTSANSINPA
                     RLLAQIGYYLVGVAIWRQQTGRTDAPTITVPCGNFGNLTAGLLAERLGLPVRRFLIAT
                     NANTVVPEYLAGAPYTPRASVATLANAMDVGAPNNWARIRALYQNDDQRLRQALTADS
                     VDDHGIRQAILDLSHQGYLADPHTATAWQVFERHRRPDEPGIFLATAHPAKFSEVLRD
                     ILHRDIPCPPVLHETLSRPLLTEDLEGSADELARRMERFCQAGAPAGNISPT"
     gene            5780..7003
                     /locus_tag="PKO06_00900"
     CDS             5780..7003
                     /locus_tag="PKO06_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV68224.1"
                     /translation="MNEQSKVQAFLHRFFQIRVPVATGESAGLGIAPIILLLFCWLAL
                     TWGRNERVIVPEGLLEGKTPVLFEMVADGTYLNIPDASLIARESSDGNVLMFVEYRDK
                     QYPLQTLPARYDSSERVVRVKLRRGMFVDRVYEQATETVSLGRGGPTARKGVALLTYQ
                     FESVETRLVSASILPSPAEVMRSARGLVVDRSLVSNVWSSFWRVLKGFVIALLFVFPL
                     GLCMGTFSRVRCMFAPLMTFAGYTPIPALVPLTMLFAAMLREFVMAVTGVTMTDEMQK
                     VLFLALAFGIYLLPLIVRSLEEVDNVYLQTAYTLGASRAQVMWRVLLGIAMPHIYDAG
                     RLGFGVGWSYIMLVEMVDLGGGGVGSLILNSQRIGPREHIFLILLVIVVLAFITDKIW
                     ELFGDWLFPYRRMKR"
     gene            7000..8271
                     /locus_tag="PKO06_00905"
     CDS             7000..8271
                     /locus_tag="PKO06_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV68225.1"
                     /translation="MTKPTDPNPPKSPSMLAKLKNLFWSDGKSSEPPTAATPARAEKV
                     VPTAPTKTSPTDSKTNSPPTTSATGTINPHASTPTSGAAPVSQPPTGTATPAQTPTAA
                     DTASFPTPVGAPTQPATPPTPGSSPAPTGASTAATTPPLDMGPIKPKPHVVEFRNVSK
                     VFNQGTPQEFCAIKDITFHIEDLPDVGEFIALVGPSGCGKSTVLNLIEGFPEVFPPTT
                     GEILVRGQPVTGPGKDRGMIFQKYSSFPHLTVLQNVLFGLEINSSENQYPAAEMKTRA
                     LDMIRKVGLAGHEHKYPYQLSGGQQQRVAIARTLVLKPRIILMDEPFSALDEPTRIEM
                     QSLITHLWKEVEATVFIVTHSLAEALYLGDRIFMFTPNPGRLGRISYIKPEDIGKDPG
                     KSPLEVQEGEKFKKHLAHITSEFLRLQEGKH"
     gene            8271..9188
                     /locus_tag="PKO06_00910"
     CDS             8271..9188
                     /locus_tag="PKO06_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68226.1"
                     /translation="MPQEPLGYLDYLGEAFHASPPVKGMGPVPVNKVFLAGAFILGFA
                     NPGFWALAAALELLYLYFLSTHPAFQKYVQAKRLKIVDASRAEKINSMILSLDPASKR
                     RLNALNANLNEITRLMNIDSDSASSFLRETKQRTLQQLSDVFLRMTFSKRVINDSLQN
                     TDVDKIKREAKEIEMQIQKPGISEALARSHKANLEIQVRRITNIEKANENLDLVEAEL
                     KRIENQVLLIREEVAINRSPEALSSTLDRIGNTLSETEDWMSKHSDFFSKVAGSDDEL
                     PIPLPASSVSTEEAEDPGNVPPPQTPVSQ"
BASE COUNT         1812 a         2956 c         2617 g         1926 t
ORIGIN      
        1 aagatcctga cgacgaccag cgaagccaac aagctcatcg ccgatgtctg ggccgtgcgc
       61 gccgacttct atcgcgataa ccccgagatc gtgaagggcc tcgtcgaagg catcttcgaa
      121 ggcatggaaa tgctgaagag ccagaccacc aaagcgcagg cattccagtg gatggccgac
      181 ggcttcggca tgaaggtcga tgatatcaag ggcatgcaga acgatgctca ctcgacgaac
      241 ttcgcggaaa accgcgattt tttcctgaac gccaacaacg ccacgaactt cgaacgcacc
      301 tggaacaaca tctgtttcgt ctacaaagaa atcggactga tggacaatct cgttccgttt
      361 gatcaggtca tggatttcag catcgtcaag aagctggcgg aagagggcaa gttcaaggaa
      421 cagcagaacg agtatgtcag tcgcttcgtg ccaaccacct acaacaaggt gcaggccgaa
      481 gcgccgatca tcacccagac ggtgcgcatc aacttctatc cgaacagcgc taacatctat
      541 gagccgaagc atgatgatat cggccggccg atgccgaaca ccttgtacga tgaatcgatc
      601 gacgcaaccc tcgagaaggt cggacgtctg gtcggtgcct atgatcgcgc ggtggtggcc
      661 attgtcggtc ataccgacgc ttccatgaaa ggcaagattc ccgagcagat cgtgaaagat
      721 ctgagcatgg atcgcgcgaa ctcggtcaag gaagctctga tcaagaagtt ccactttgat
      781 cccaacaagt tcgtcgtcga aggcaaaggc tgggatcaac ccgccgaatc ctccgatccg
      841 accaatcaca cgatcaatcg acgcgtcgaa gtttccgtgt attcacctga gggtggaaac
      901 tgagtcagtc tgacaacgac cggatgacgg gcggggcgct ggcgctccgc ccgttttttc
      961 cggaatcagg ttcggaggca ggatcatgaa ggtcatgaag ttcggcggca catccgtcgg
     1021 ggatgcggat cgtctgcgca acgtcgccag actggtcgcg gcagcggcgg ttcgagaacc
     1081 ggtgggtctg gtcatttccg ccatgacagg tatcaccaat catctgatcg acagcgtgac
     1141 cgcagtattg gcagggaagg atccactctc attcgtccag gttttcgaag gtcgtcatca
     1201 gcaggcattg cagtttttgc aggggtcggt ttctgacttt cctctcgaaa aactgcagcg
     1261 tgagctttcg gctcacgtcg acgagttttc cagcctgctc aacggggtgc gcctggtcga
     1321 cgagtgctca gcccgtaccc tcgaccgcat cagctgtctg ggtgagcgcg cgctgattcc
     1381 ggccatggcg gccttattac aggcagaagg tctgtcggtc aaagtgattg atccgcgtgt
     1441 catggtgctg accgacagcg tcttcgggaa tgcctccccc ctgatggacg aaatcgagcg
     1501 acgcttcgcc tcggagcgcg acctgcatcc ccgcgaggtt ctgctcatgc cgggcttttt
     1561 cggcggcgat cgtcgaggtc actacacgac actgggtcgg ggaggatccg attattcggc
     1621 ggcgattctg gcagccgctc tgcatgccga cactctcgaa atctggaccg atgtcgacgg
     1681 tgtctacact gccgatccgc gcgtcgtccc cgacgcgttc gtcctcgagg aaatgaccta
     1741 taccgaagcc atggaactgg cgttcttcgg tgcgaaggtc ttgcatccgc gcacgatcgc
     1801 ccccgtcgtc gaacggtcga ttcccgtcat catccgcaat actttccgcc ccgaccaccc
     1861 cggcacacgc gtggcacgca cgaccactgc cggaaagcac gccattcgcg ggctctcttc
     1921 actcggtgcc gtggccatga tcggtctttc gggtgccggc ttacgcggcg tcccgggagt
     1981 tgccgcccgt gtattcagtg ccatggcgca cgccggtatc tcggtcatct tgattgctca
     2041 ggcgtcgtcc gagtattcca tctgtttctg cgtcagcgaa ggacgcgctc ctgatgcccg
     2101 cgagacgctg gaagaggagt tccgggtcga gagacaggcg ggtctcgttg atcccatcga
     2161 agtggtccct tcgttagccg tcatctcggc cgttggcgaa gaaatgcgct cgcggcgagg
     2221 gttggccggc gcattcttcg gcggtctggc cacggcggac gtcaacgtgg tcgccatcgc
     2281 ccaagggttt tcagaacgca acatctcggc cgtgatcgcc ggagcggaca cccagcgcgc
     2341 catgcgagcc gcgcatcagt cgtttttcaa caccacccag ggcatccagg tctttctcgt
     2401 gggtgttggc gcggtgggcc gcgagctggt tcagcagatc gaaacgcagc aggcaagcct
     2461 gcgacaacag ggtatcgatc taaggctgtg cggcgtggcc gactcgcgcc acatggcgat
     2521 caatctcgaa gggtataagt caggagcctg gcaaaaggct ttactcgaga gctcgacggc
     2581 aaccgatctt cgtcagctcg cgtcgattgt ccaggatgct cgactgatga atccggttct
     2641 ggtcgacgcc acaggcagcg ataagattgc ctcagcctat ctccccctgc tcgagtcggg
     2701 catgcatgtc gtcaccccga acaagctggc gaacgccggt tcctgggatt actacctggc
     2761 gctgcgccag acggcgaacc ggcggcgacg tcactttctt tacggcacga atgtcggggc
     2821 cgggctgccg atcatcgata ccctgcgcaa tctgcggcgc agtggcgatc gattgcgagc
     2881 ctgcagcggc atcctatccg gctcgctttc gtttctcatg ggacgactcg aagatggagt
     2941 tcccttttct caggttctgc acgaagcccg cgatcgcggc ttcacggaac cggatccgcg
     3001 ccaggatctt tcgggcatgg atgtcgcccg caagattctg attctggctc gagaggccgg
     3061 tcttcgacgt gagcttgccg aaattcgcct cgaatcaatt ctgccggctg gctctctaac
     3121 cgatgaaccc gtcgaggcct ttctcgcccg cgctccagat ctcgacgcgc acttttcccg
     3181 actcacggcc gaagcaaaac gcgagaacaa ggtctggcgt tttgccggtc gcatcgaaac
     3241 agacgggtcc tgtcacgtcg gccccatcgc cgtgacgcac gatcaccctc tggccgcggt
     3301 tcgcgacggc gagaacgccg tcagtctgct cacagaccgg tattctccga ttccgctggt
     3361 tgtccgcggg tatggggccg gtccggccgt cacggccgca ggcgtctttg ccgatctgat
     3421 gcgaaccgtg atctggcata ccgagggcgt actgtcgtga ggcccggcgg tttctgtcgc
     3481 gctttcgccc ccgccaccat cggcaatctg gccgctggat tcgacgttct cggcgtgtct
     3541 ctggccccgg tggacagcac gttgtggggt gacatcgtca cgattcgcgt cccgcgcact
     3601 ccgcgccgag cggatcgttt caccgttacc ggacctcatg cgaacgcggt tcccagtcgc
     3661 accacagaga atctggtgat gatcgcccgc cgcctcttca atcaggaact gcgacggcga
     3721 caactgccaa cgaccggtct ggaactctgc ctcgaaaagt gcctgcccgt ctgcagcggt
     3781 cttggctcat cggccagttc agtggttgcc gcgctcatgg ccctgaacac cattcatgaa
     3841 tctccgctcc ccacgccgac cctgctcgaa ctggccggtc gagcggaagg gcatgtcagc
     3901 ggatcgattc atctcgacaa cgtcgctccc tcgatgctgg gcggaattca gctcctggtt
     3961 cccggtacaa acaatgtcct tcagcctgtc ccgctgccgt ggttctcgca ctggtatctg
     4021 accgtcgttc atcctgatct gcaggtaccg accgctttgg ctcgtcaggt gctgccgccg
     4081 gcattttcac gcgctgcagc cgttgattac tggtcgaatc tggccggtct ggtgcatgcg
     4141 ctgcacaccg gcaacgagtc cctggcggcg cgttgccttc gcgacccgtt gatcgaacca
     4201 catcgtcaat cgctggtaaa gggattcgat cgggtcaaac gcgaagccct cgctgccggc
     4261 gcactcggat tcagcctttc cggctcgggt ccctcagttt tcgcggtcac cgaaagcctg
     4321 agtcgggcag aagagttgct ggatcgcatg caacaggcgt ttcaacgcgc gcgcgtcacc
     4381 agtcagggtc acgtctgtcg cccagacaac catggcgcga gaatcattga aagcgactat
     4441 tcataattat gatctggtac tctctcagtc aaccatccga caccgcgacg ctgctcgaag
     4501 ccgctcaacg cagtatcggt catgatggcg gcctcttcat gccccgtgac tttccgcgct
     4561 gggatgatgt caatcgtttg ctgactctgc cgccccgaga gctctggaga gaaatctttc
     4621 agcgccttct ggtcggcgaa ctttcctcca gtgaaataca gagcctgacc gatgaggcgt
     4681 tttcctttcc attgcctctc acccgcgtca gcgaacacgc ctggtctctc gagttgtttc
     4741 atgggccgac cctcgccttc aaggacttcg gcgcgcgctt tctggcgcga atcctcggcc
     4801 acgctacccg ccaccagacc gggaagccgc tgaccattct gacggcgacc tcaggagata
     4861 caggagccgc cgtggcgcag gcgtttcatc gcgtgcccgg aattcgcgtg gtggtactgt
     4921 ttcccaaaca ccggatttcc cccctgcaag agcgcctgtt cgccaccctc ggcgacaatg
     4981 tgctggcact gcgtgtggct ggaagttttg acgactgtca gagtctggtc aaggcctgct
     5041 ttcgcgatcg cgagctgtgc cggcacttcg gcctgacttc ggcgaacagc atcaatccgg
     5101 ctcgtttgct ggcacagatc ggatactacc tggtcggcgt cgcgatctgg cgtcagcaga
     5161 ccggtcgcac cgacgctccg accatcacgg ttccctgcgg gaacttcggc aatctgacgg
     5221 ccggtcttct ggcagaacga cttggtctgc cggtgcgccg attcctcatc gccaccaatg
     5281 ccaacaccgt cgttcccgag tatctggcag gtgcgcccta cacgcctcgg gcttcagttg
     5341 ccacgctggc gaacgccatg gatgtcgggg ctcccaacaa ctgggcccgt atccgcgccc
     5401 tgtatcagaa tgacgatcag cgtctgcgtc aggcgctcac cgccgacagt gtcgatgatc
     5461 atggcattcg tcaggcgatt ctcgacctct cccaccaggg gtatctggcc gacccccata
     5521 cagcgaccgc gtggcaggtt ttcgaacgcc atcgccgccc cgatgaacca ggcatctttc
     5581 tggccaccgc tcatccagcc aagttttccg aggttctccg ggacatcctg caccgcgaca
     5641 ttccctgtcc gccggtattg cacgaaactc tgagtcgacc ccttcttaca gaggatcttg
     5701 agggttccgc cgacgagctt gcccgtcgaa tggaaagatt ttgccaagca ggcgcccctg
     5761 cgggtaatat atcccccaca tgaacgaaca atcgaaagtc caagctttcc tgcatcgctt
     5821 tttccagatc cgggttcccg ttgccaccgg agaaagcgcc ggtctcggta tcgccccgat
     5881 catccttctg ctgttctgct ggctggccct gacctggggg cgaaatgagc gcgtgatcgt
     5941 tcccgagggc cttcttgagg ggaagacgcc ggttctgttc gagatggtcg ccgacggcac
     6001 ctatctcaac attcccgatg cctcgctcat tgccagagaa agctcagacg gcaatgtcct
     6061 gatgttcgtc gagtatcgtg ataagcagta tcccctgcag actctcccgg cccggtacga
     6121 ttcctcggag cgggtcgtac gagtcaaact gcgccgcggc atgttcgttg accgcgtgta
     6181 cgagcaggca accgagaccg tcagcctggg gcgcggtgga ccgactgcca gaaagggtgt
     6241 ggcactgctg acctatcaat tcgagagtgt cgagacccgc ctggtttccg cctcgatcct
     6301 gccgagtccc gccgaagtca tgcgctcggc acgggggctc gtggtcgacc gcagcctggt
     6361 gtcgaacgtc tggtcgagct tctggcgcgt cttgaaaggg ttcgtcatcg cgttgctgtt
     6421 cgtgtttcca ctggggttgt gcatgggtac gttttcgcgc gtccgatgca tgttcgcacc
     6481 gttgatgacg tttgccggtt acacgcctat ccccgcgctg gtccctctga ccatgctgtt
     6541 tgcggccatg ctgcgcgagt ttgtcatggc ggtcaccgga gtcacgatga cggatgaaat
     6601 gcagaaagtg ctgtttcttg ccctggcctt cggcatctat ctgctgccgt tgatcgtgcg
     6661 atcgctcgaa gaggtcgaca acgtctatct gcagaccgca tacacgctgg gcgccagtcg
     6721 ggctcaggtc atgtggcgcg tgctgctggg cattgccatg ccccatatct atgacgccgg
     6781 tcggctgggt tttggagtcg gctggagcta catcatgctc gtcgagatgg tcgatctggg
     6841 cggcggcggc gttggctcac tgattctcaa cagccagcgc atcggcccgc gcgagcacat
     6901 ttttctgatt ctgctcgtga tcgtcgtgct ggcgttcatc accgacaaaa tctgggaact
     6961 gttcggcgat tggctgttcc cctatcggag gatgaagcga tgaccaagcc aaccgatccc
     7021 aatcctccca agagtccgtc catgctggcg aaactgaaaa acctcttctg gagcgacggc
     7081 aagagttctg aaccgccgac cgcggcgact cctgcgcgag ccgagaaagt cgttccaacc
     7141 gcccctacaa aaaccagccc aaccgacagc aagacaaata gcccgcctac tacctcggca
     7201 actggtacta tcaaccctca cgcttcgact ccgacttccg gtgccgcacc cgtctctcaa
     7261 ccaccgaccg gcaccgccac accggcacag actccgacag cggcggacac cgccagcttt
     7321 cccaccccgg ttggcgcgcc cacgcagccc gcgacccctc cgactccggg aagttcgcct
     7381 gcgccgaccg gcgcatcgac cgccgcaaca acgccaccac tcgacatggg accgatcaag
     7441 cccaagccgc atgtcgtgga gttccgcaat gtctcgaaag tcttcaatca gggcaccccg
     7501 caggaattct gcgccatcaa ggacatcacg ttccatatcg aagacctgcc cgacgtcggc
     7561 gagttcatcg ctctggtcgg cccatccggt tgcggcaaat cgacggttct gaacctcatc
     7621 gaagggtttc ccgaagtgtt cccaccgacg acgggtgaga ttctggtgcg cggccaacca
     7681 gtcaccggcc cggggaaaga ccgcggcatg atctttcaaa agtacagttc attcccgcat
     7741 ctcacggttt tgcagaacgt cttgttcggc ctcgagatca actcctcgga gaatcagtat
     7801 ccggcagccg agatgaaaac ccgcgccctc gacatgatcc gcaaggtcgg gttggcaggc
     7861 cacgagcaca agtatcccta tcagctgtct ggcggtcagc agcaacgcgt cgccatcgcc
     7921 cgcacgctgg tactcaagcc gcgcattatt ctcatggatg aaccgttctc ggcgttggat
     7981 gaaccgaccc gcatcgagat gcagtcgctg atcacccacc tctggaagga agtggaggcc
     8041 acggtcttta tcgtgaccca ctcccttgcc gaagccctgt atctcggtga ccgcatcttc
     8101 atgttcactc cgaatcccgg tcgtctgggc cgcatcagct atatcaaacc cgaagatatc
     8161 ggcaaagatc ccgggaaatc gccgctcgag gtacaggaag gcgagaagtt caagaagcat
     8221 ctcgcgcaca tcaccagtga gtttctgcgg ttgcaggaag ggaaacactg atgccacagg
     8281 aaccgctggg ttatctcgac tacctgggcg aggcttttca tgcctccccg ccggtcaaag
     8341 gcatggggcc ggtcccagtc aacaaggtct ttctggccgg cgccttcatt ctcggtttcg
     8401 ccaaccccgg tttctgggcc ctggccgccg ctctcgaact cctctacctg tactttctct
     8461 cgacacaccc ggcgtttcag aaatatgtac aagccaaacg cctgaaaatc gtcgatgcct
     8521 cgcgggccga gaaaatcaat tccatgattc tcagcctcga cccggcctcg aagcgacgcc
     8581 tgaatgctct caacgccaat ctcaacgaga tcacgcgtct gatgaacatc gactcggaca
     8641 gcgcttcgtc gtttctgcgc gagaccaagc agcgcactct gcagcagctt tccgacgtct
     8701 ttctgcgcat gaccttcagc aaacgcgtga tcaacgacag tctgcagaac accgatgtcg
     8761 acaagatcaa gcgcgaagcc aaagaaatcg aaatgcagat tcaaaagcca ggcatcagcg
     8821 aagccttggc ccggtcgcac aaggcgaatc tcgagattca ggtcaggcgc atcaccaaca
     8881 tcgaaaaggc gaacgaaaat ctcgatctgg tagaagccga gttgaagcgc atcgagaacc
     8941 aggttctgtt gattcgcgaa gaagtggcca tcaaccgcag tcccgaagcg ctttcctcga
     9001 cgctcgaccg cattgggaac accttgtcgg aaaccgaaga ctggatgagc aaacattccg
     9061 actttttcag caaggttgcg ggcagtgatg atgaactccc gattccgctc ccggcctctt
     9121 ccgtctcgac cgaagaagca gaggaccccg gcaatgttcc gccgccgcag accccggtca
     9181 gtcagtgaca tgagcgacct cggcgaccgt ctgccgctct cccgccgccg ttcccgtttt
     9241 ccccgccagc cgctccgaac gctcccggtc ttttcacgag actcggcgcg acatttcacc
     9301 accctcgcag a
//
LOCUS       DAORHD010000019         9120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7039_length_9120_cov_4.777606, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000019 DAORHD010000000
VERSION     DAORHD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7039_length_9120_cov_4.777606"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKO06_00915"
     CDS             <1..273
                     /locus_tag="PKO06_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HNV68227.1"
                     /translation="REAQKIPSGQRQELCRGLHAEQNAILQAAAFGVSLKGAELYCTH
                     QPCVTCAKMLINAGVEKIVFLGDYPDSLSLEILEEGKITLERIRLE"
     gene            277..1545
                     /gene="murA"
                     /locus_tag="PKO06_00920"
     CDS             277..1545
                     /gene="murA"
                     /locus_tag="PKO06_00920"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV68228.1"
                     /translation="MSRIVIEGGHRLSGVVRVGGGKNATLPIMAASILSAGPLVLENV
                     PRLNDVDTMGKVINALGVNTERLPNGDWKLHSPGGVREEAPYELVKAMRASFFVMGPL
                     LARLKKARVPLPGGCAIGVRPVNIHLKGFEALGARVSLEAGCAVAVADRLVGARILLD
                     FPSVGATENLMMAACLAEGTTLIENAACEPEIADLAGFLNAMGGRVTGAGTPTITIEG
                     VKELGECRYRIMPDRIDGGTFLVLGAMGTSPITVENVVASHQQAVITKLQEMGAELEI
                     EENRITVSRKGRLKPVEFKTLPFPGFPTDMQAQFMVALCLAEGTSIINETVFENRFMH
                     VAELSRMGAQIAIRDRTVVINGVEKLNGAPVAATDLRAGAALVMAGLNAEGRTEVSNV
                     HHIDRGYEDLVPRLMGLGAQIKRLDGNGHG"
     gene            1590..3293
                     /locus_tag="PKO06_00925"
     CDS             1590..3293
                     /locus_tag="PKO06_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68229.1"
                     /translation="MTPEAKPFKEILEGLTSRDRRQKLIHIDAVGRLRRQEHADMLVD
                     LLASPDQEVVGRVIDALGRIGNPRSLKYLLEFVVGDNVEFSDRAMQALTRFDIHASFE
                     TILRICTVERPNQVRRKLLSLLAPVKDPRVALAMNEVLGQTQDPGLLAEALGYFVQYP
                     SADKHTVLKMLSGSGQWEVAMTANLALSRLADESARSQIKRLAKSPAHPIRQAIVTGL
                     NKSPMNEDRELFEVFFKDQHPQIRHLAMAGLDLFAAPERATLINDWLQREKDDTVRFD
                     LLQRAAAEKSPVFYTEFVKLLSSPNDQYKRLARQALIDMGSSALDRILSEYPKMSLVL
                     KEQMILVLGGIGHERGRPAILEALRGKERWLRLNAIEALAQVGEHSLARRFEEMLRAE
                     KDDWVIATLASILGRVGNRDQIPLISDCLSHRDARVRANSVESLGMLAGPECRDQLQP
                     FLRDPNDRVRVNAAIVLWRLGDREVTGILKELIKDSNKWVRASAAFALGEIADPDAVP
                     VLLTALVDKEEVVYKNVLEALAKIGDVRALVPLLEERERKRLPDAALDDVMERFSEHL
                     K"
     gene            3354..4187
                     /locus_tag="PKO06_00930"
     CDS             3354..4187
                     /locus_tag="PKO06_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68230.1"
                     /translation="MNFEDQEQKRRERDDLLHGRGTKRTGETFSPIADEEEFRQNRPS
                     CDAVESSPNLDHEEMALLGRVKVDLDTPLEKGLSFAMWALLFGAYMCFHIGVFGGKRS
                     PPSPEFLTGLPYFLGFAALAFALRRGTDNYYLLDIRARQVKYHFRFLNWESVETAFRF
                     DDILWVEYRIETRRHKSNTWNEHQLRVHRSIGEDVEFSNWERDGDNLRSLGQKLAKLF
                     QRPLKTADLSELSENPKSDGSNVLFHVGETPVTEHRLAITIGLLFLLTVIGIIVLSAL
                     L"
     gene            complement(4334..7564)
                     /locus_tag="PKO06_00935"
     CDS             complement(4334..7564)
                     /locus_tag="PKO06_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV68231.1"
                     /translation="MELNFSGRFVGKFVNSKLTPLFVITSLLLGYFSLAVIPREEEPQ
                     IVVPMVDIFVGMPGASPAQVENRVVRPLEKLIWEIPGVEYVYSTSGEEQAMVIVRFKV
                     GEDLERSLVKLSEKLEHHKDRIPPGVIGPMVKNRTIDDVPFLSLTFWSRQYAHNDLRR
                     VAAQVQDALKAIPDVSVTTITGGMPRSVVVQPDPGKLAGYGVSLFELTSALRSANVEF
                     SSGSFDRDNMNVPLKTGRFLSSRWDVENLVVAVRDRKPIRLLDVAQVIDGTDEPDNYV
                     FIDFGSAGSPESATQSPDLTNGGPAPAVTLSLAKRKGTNAISVGDLVRQKLEVLKKNI
                     IPEEVQVLVTRDYGKTAQEKANDLFTSMGQAVGLVMIILFLTLSLRVALVSSVSIPVT
                     LSLVMLAFYAFGYTLNRITLFAMIFSIGLLTDDAVVVLENMIRHIGMTKNRGKDPALI
                     AQEAVHEIGDPTILANIAIVLAVMPMALVSGMMSPYMEPIPVGTAAAAMLSLLVAYVV
                     TPWAAIRFVKFPEPSAANDTPEEPPDTIFERVYRKIITPLVKRPKLRVGFIGLAWGIV
                     LIAILLVPLKIVKIKILPFDNKSEFQVVIDMPEGSTLEQTAAVTKEISEYLKTVPEVT
                     DVEMYIGNSGPFNFNGLVRHYFLRQGPNVADIQVNLVHKDKRTAKSHDIALRVRPPIQ
                     EIARRYKANAKIAEVPPGPPVLQTLVIEVFGPTEQGRKEAAASIRALLEQTPGVVDVD
                     TYVEDDQKQLIFDVDQTKAALLGLSPEQIAQALRLSVSGMQVGLMNMPREKEDVALLV
                     RLPKPDRSGVSALLNLHVASPATGRSISLGEVITPRERIRDKSLYHKNLQPVVYLTAD
                     VAGSEESPVYPIVNLIQSISQVKAPDGGEIVQWHTQVPAETISGYSMKFDGEWQITYE
                     VFRDLGLAFAAVMILIYFLMVGWYQDFITPIGILLPVPFSLAGVLPGHALLGAFFTAT
                     SMIGFIAGAGIIVRNSLLLIDFIIELRNRGDTLENAVINAAVIRFRPMVLTSLSTLIG
                     ATVMLTDPIFQGLAIAIVFGEIASTCLTRVAVPVIFYQIEIWRGRS"
     gene            complement(7580..8743)
                     /locus_tag="PKO06_00940"
     CDS             complement(7580..8743)
                     /locus_tag="PKO06_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV68232.1"
                     /translation="MNHHHPFKRRSFLRLAMFSIAAVLTFASGGFTQGSTPEWTTAAR
                     KTIPVSEEVVGILQPTTVTTLSSKVMGNVVEVLKREGETVAAHELLVRIDAKEIGSDL
                     AGAKATLAEAQAAIAEIRSTINTAQAAKEAAESQLQLAEVSYARIKELYDKKSTTQQE
                     HDQAFTRLTQAKSQVTQAASQISSLQAKLAQINARNEQARAGISKVSTLKELAEVRAP
                     FAGRIISRKVEPGMLAAPGVPLMIIEDLGRIRFEAIVPERLLSYLPDGASMTVRVDAL
                     GPELFSGTVAETVPGGDPMSHTFTVKFTLTDDARFRTGMYARGAVLKGEEQVLLVPTN
                     AVVSRGQLDGLFVKSGEKSLYRLVKTGRTIGDEVEILSGLNEGDQYDRRPTSR"
     gene            complement(8776..>9120)
                     /locus_tag="PKO06_00945"
     CDS             complement(8776..>9120)
                     /locus_tag="PKO06_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV68233.1"
                     /translation="VFSFVVSLRPSEREPISNINNALERIRVSREAIGQSEESLRILR
                     DRYVAGLAIVSELLGAETSLLSHKMNHLNALYDYSISRARLKLALGDLTVEKCDILQP
                     EAASVEMPSQTK"
BASE COUNT         1923 a         2435 c         2750 g         2012 t
ORIGIN      
        1 cgcgaagctc agaagattcc ttccggccag cgccaggaac tctgtcgggg attgcatgcc
       61 gaacagaacg cgattcttca ggcggcggcg tttggggttt cgctgaaagg cgccgagctg
      121 tactgcacgc accaaccctg tgtcacgtgt gccaagatgc tgatcaatgc cggggttgag
      181 aagatcgtgt ttctcggcga ttatcctgac tcgctttcac tcgagattct tgaagaaggc
      241 aaaatcacgc tcgaaaggat tcggctggaa taagctatgt cacgtattgt catcgaaggt
      301 ggtcatcggc tttcgggtgt tgtgcgtgta ggtggcggca agaatgcaac tctgccgatc
      361 atggcggcgt cgattctttc ggccggtccc ctggttctgg aaaacgttcc gcgcctgaat
      421 gacgtggata ccatgggcaa ggtcatcaac gctctcggtg tgaacaccga acgtctgcca
      481 aatggtgact ggaagctgca ttctccgggt ggagtcagag aagaagcgcc ctatgagctg
      541 gtgaaggcca tgcgagccag tttctttgtc atggggccac tgctggctcg tctcaagaag
      601 gctcgagtgc cgctgccggg cggttgtgcc attggcgtgc ggccagtgaa catccatttg
      661 aaaggcttcg aagcgctcgg cgcacgtgtt tccctggagg cgggctgtgc cgtggccgtg
      721 gccgatcgct tggtgggcgc gcgtattctg cttgacttcc cctctgtggg tgccaccgaa
      781 aacctgatga tggcagcgtg tctggccgaa ggaacgacat tgatcgagaa tgcggcctgc
      841 gaaccggaga tcgccgatct ggccggtttc ctgaatgcca tgggaggtcg cgtcaccggc
      901 gcgggaacac cgaccatcac gattgaaggc gtgaaggaac tgggcgagtg tcgctatcgc
      961 atcatgcccg atcgcattga tggcggcaca ttcctggttc tgggcgctat gggcacttca
     1021 cccattacgg ttgaaaacgt cgtggcttct catcaacagg cggtgatcac caagctgcag
     1081 gagatggggg ccgaactcga gattgaagag aaccgcatta ccgtttctcg caaagggcga
     1141 ctcaagccgg ttgagttcaa aaccctgccg tttcccgggt tccccacgga catgcaggcg
     1201 cagttcatgg tggcgctgtg tctggccgaa ggcacgtcga tcatcaatga gacggtcttc
     1261 gaaaaccgct tcatgcacgt cgccgagctg tcacgcatgg gggctcagat tgccattcgc
     1321 gatcgtaccg tggtcatcaa cggggtcgag aagttgaatg gggctccggt tgcggccacg
     1381 gatctgcgcg cgggtgcggc tctggtcatg gctgggttga atgcggaagg gcgcaccgaa
     1441 gtgagtaatg tccatcatat cgatcgcggt tacgaagatc tggtgccgcg actcatgggg
     1501 ctgggtgccc agatcaagcg cctcgacggc aacgggcacg gctgaacgaa gctgatgccc
     1561 atgaacacac tacgagcggg agggagccga tgaccccgga agcaaagcca ttcaaggaaa
     1621 ttctcgaggg cctgaccagt cgagatcggc gtcagaagct gattcacatt gatgcggtcg
     1681 gtcgtttgcg ccgacaggag catgcggaca tgttggtcga tctgctggct tcccctgacc
     1741 aggaggtcgt cgggcgagtg atcgatgcgc tcgggcgcat cggaaacccg cgctcgctca
     1801 agtatctgct cgagttcgtc gtcggtgaca acgtggaatt ttctgatcgg gccatgcagg
     1861 cgctgactcg ctttgatatt catgcctctt ttgaaaccat actgagaatc tgtacggttg
     1921 agcgaccgaa tcaggtgcgg cggaaactgc tcagcctgct ggctcccgtc aaagaccccc
     1981 gggtcgccct ggccatgaat gaagtccttg gccagactca ggatccgggg ctgctggccg
     2041 aagcactcgg atactttgtt cagtatcctt ccgcagacaa acataccgtt ttgaagatgc
     2101 tttcaggcag cggacaatgg gaagtggcca tgacggcgaa tctcgcgctg tctcgtctgg
     2161 cagacgaatc ggctcggtcg cagatcaagc gactggcgaa atcacccgcg caccccattc
     2221 ggcaggcgat cgtcacgggt ttgaacaagt cgccgatgaa tgaagatcgt gagttgttcg
     2281 aagtcttttt caaagaccag catccgcaga ttcggcatct ggccatggcc ggacttgatc
     2341 tgttcgcggc tcccgaacgg gcgactctga tcaatgactg gctgcagcgt gagaaagacg
     2401 acaccgtgcg tttcgacctg ctgcaacgcg ccgcggccga gaaaagtcca gtgttctaca
     2461 ccgagtttgt gaagctgctt tcctcaccca acgaccagta caaaagactg gctcgtcagg
     2521 cgttgattga tatgggatca agcgctctcg accgcattct gagcgagtat ccgaaaatgt
     2581 cactggtact caaggaacag atgatcctgg tactcggcgg cattggtcac gagcgaggtc
     2641 ggcctgcaat tctcgaggcg ttgcgcggca aagagcgatg gttgcgtctg aatgccatcg
     2701 aggctctggc tcaggttggt gaacacagtc tggcacgccg gttcgaagaa atgttgcgag
     2761 ccgagaaaga tgactgggtg atcgcgacct tggcatcgat tctcggacgc gtgggaaacc
     2821 gtgatcagat accgctgatc agtgactgtc tcagtcatcg tgatgcgcgt gtgcgcgcca
     2881 acagcgtgga aagtctgggg atgctggccg gcccggaatg tcgggatcag ctgcagccct
     2941 ttttgcgtga tccgaatgat cgggtgcggg tcaatgcggc gattgttctc tggcgcctcg
     3001 gagaccgaga agtcacgggc attctcaagg aattgatcaa ggattccaac aagtgggtgc
     3061 gcgcctcggc ggcctttgcg ctcggagaaa tcgctgatcc tgatgcggtc ccggtgcttt
     3121 tgacagctct ggtggacaag gaagaagtgg tctacaagaa cgtgctggaa gcgttggcca
     3181 agatcggaga tgtgcgcgcg ctggtgccgt tgctcgaaga gcgagaacgg aagcgattgc
     3241 cggatgccgc tcttgacgat gtcatggagc ggttttccga acatctgaag taaacaattc
     3301 cggcatcggg gggcatcggt cgtggtcatc gaagcgatca ggagctgtgt cttgtgaatt
     3361 ttgaggacca ggaacagaag cggcgtgaac gtgacgatct tctgcacggc agagggacga
     3421 aacgaaccgg ggaaacattc tcaccgatcg cggacgaaga agagtttcga cagaatcgtc
     3481 cgagttgtga tgcggttgaa agctcgccca accttgatca tgaagagatg gctctcctcg
     3541 gacgagtcaa ggtcgatctc gacactcctc tcgaaaaggg gctgagtttc gccatgtggg
     3601 cgctgctctt cggcgcgtac atgtgttttc acattggggt gttcggtggc aagcgttcac
     3661 ccccgagtcc ggaatttctc accggcttgc cctattttct tggttttgct gccctggcgt
     3721 ttgccttgcg ccgcggcacg gacaactatt acctgctcga cattcgcgcg cgtcaggtga
     3781 aataccactt tcgattcctg aactgggaat cggtggaaac ggcttttcga ttcgacgaca
     3841 tcctgtgggt cgagtatcgt atcgaaacca gacgccacaa gagcaacacc tggaacgaac
     3901 atcagttacg ggtgcatcgc tcaatcggcg aagatgtcga gttcagcaat tgggaacgcg
     3961 acggcgacaa cctgcgatcg cttgggcaaa aactggcaaa acttttccaa cgcccgctga
     4021 agaccgcaga tctttccgag ttgagtgaga acccgaaatc agacggttcg aacgtccttt
     4081 ttcatgtggg cgaaactccg gtcacggaac atcgtctggc gatcacaatc ggacttctct
     4141 ttcttctcac cgtcattgga atcatcgttc tgtcggctct gctctgatga gtgaactggt
     4201 acgttggaaa gccggatgtg cctttccaga tagtactgat ccagaggtat cgtcgtgaaa
     4261 gaaaaccgcc ccccggcgag tgtggtgccg gagggcgact tcgctgtacg agtttctcct
     4321 tcgtgttctt tgctcaggat cgacctctcc agatttcgat ctggtagaag atcaccggaa
     4381 ccgcgacgcg ggtgaggcag gtcgacgcga tttcaccgaa gacgatggca atggccagac
     4441 cctgaaagat cgggtcggtc aacatgaccg tcgcaccgat cagggtcgag agcgaggtca
     4501 ggaccattgg tcgaaatcga atgacggcgg cgttgatgac ggcgttttcg agcgtatctc
     4561 cccggtttcg cagttcgatg atgaaatcga tcagcagcag agaatttcgc acgatgatgc
     4621 cggcgccggc aatgaagccg atcatcgaag ttgcggtgaa gaaggctccc agaagggcgt
     4681 gaccgggcaa aacaccggcg agactgaacg gcaccggcag cagaatgccg atcggggtaa
     4741 tgaaatcctg ataccaaccg accatgagaa agtagatcag aatcatgacc gcggcaaaag
     4801 cgaggcccag gtcgcggaag acttcatacg tgatctgcca ctctccgtcg aacttcatgg
     4861 agtacccgga aatggtttcc gccggcacct gagtatgcca ctgcacgatt tcgccgccat
     4921 ctggagcctt gacctgactg atactttgaa tcaggttcac gatcggatac accgggcttt
     4981 cttctgatcc tgccacgtcg gctgtcagat agacgactgg ctgcaggttc ttgtgataca
     5041 ggctcttgtc gcgaatgcgc tcgcgaggcg tgatcacttc acccagggag atgcttcgcc
     5101 ccgtggccgg tgatgccacg tgcagattga gcaacgctga aacgcctgag cggtcgggtt
     5161 tgggcagtcg taccagcaga gcgacatctt ccttttcgcg tggcatgttc atgagtccta
     5221 cctgcatgcc gctgacagac agtcgcagag cttgtgcaat ctgttccggt gacagaccga
     5281 gcagtgctgc cttcgtctgg tcgacatcga agatcagttg tttttgatcg tcttcgacat
     5341 aggtgtcgac gtcgacgacg cccggggtct gttcgagcag tgcgcgaatg ctggcggctg
     5401 cttccttacg accttgttcg gttgggccga aaacctcgat gaccagagtt tgaaggacgg
     5461 gtggtccggg tgggacttcg gcgatctttg cgttggcttt gtaccggcgg gcaatctcct
     5521 gaatgggcgg tcgaacgcgc agagcgatat cgtgactttt ggcggtacgc ttgtccttgt
     5581 gaacgagatt cacctgaatg tctgcgacgt tgggtccctg acgcaggaag tagtggcgca
     5641 ccaacccatt gaagttgaag ggtcccgaat tgccgatata catctcgacg tcggtgactt
     5701 ccggaaccgt cttcagatac tcggaaatct ctttggtcac ggcggcagtc tgctcgagtg
     5761 tcgagccctc gggcatgtcg atcaccacct gaaattcgct cttgttgtcg aacggcagaa
     5821 tcttgatctt cacgatcttc aacggtacca gcagaatcgc gatcaacacg atcccccagg
     5881 ccagaccgat gaaaccgacg cgcagcttcg gtcgtttcac gagcggagtg atgatcttgc
     5941 gataaacgcg ctcgaagatc gtgtcgggcg gctcttcagg cgtatcgttg gccgcggaag
     6001 gttcaggaaa cttcacaaaa cgaatggccg cccacggtgt cacgacatag gcgacgagaa
     6061 gcgaaagcat tgccgctgcc gcggtgccaa ccggaatcgg ttccatgtag gggctcatca
     6121 tgccgctgac cagggccatc ggcatcaccg cgagaacaat cgcgatgttg gcgagaatcg
     6181 tcgggtcgcc gatctcatgt acagcctctt gcgcgatgag agcggggtct ttgccacggt
     6241 tcttcgtcat gccgatgtgg cgaatcatgt tctcgagcac cacgaccgcg tcgtcggtca
     6301 gcaaaccgat cgagaagatc atggcaaaga gcgtgatgcg gttgagcgtg tagccgaaag
     6361 cgtagaacgc cagcatgacg agcgaaagag tgaccgggat gctgacggaa ctgacgagag
     6421 cgacgcgcag cgagagtgtg agaaacagaa tgatcatgac cagaccgacg gcttggccca
     6481 tgctggtgaa tagatcattc gccttctctt gcgcggtctt cccgtagtct ctggttacga
     6541 gaacttgtac ttcttctgga atgatattct tcttcagcac ctcgagcttc tgacgtacaa
     6601 ggtcgccgac cgaaatcgcg ttcgttcctt tgcgtttggc caggctaaga gtgacagcag
     6661 gagccggacc cccattcgtc aagtctggac tctgggttgc ggattccggt gagccagctg
     6721 aaccgaaatc gatgaaaaca tagttgtcag gctcgtctgt tccgtctatc acttgtgcga
     6781 catccaacag tcgaatcggt ttacgatcac ggacggcgac gacgagattc tcgacatccc
     6841 agcgtgacga gagaaatcga ccggttttca gcggaacatt catattgtct cgatcgaaac
     6901 tgccagagga gaactcgacg ttcgccgaac gcagggccga ggtcagctca aagagtgaga
     6961 caccgtagcc ggcaagcttt ccggggtcgg gttgaaccac gactgaacgc ggcattccgc
     7021 cagtgatggt tgtcaccgaa acatccggaa tcgctttcag cgcgtcctgc acctgggctg
     7081 cgacgcgacg cagatcattg tgggcatact ggcggctcca gaacgtgaga gacaggaacg
     7141 gaacgtcatc gatggtgcgg tttttgacca tcgggccgat gactccggga ggaatgcgat
     7201 ctttgtggtg ctccagtttt tcagacagct tcaccagtga tcgctcaaga tcttcgccga
     7261 ctttgaatcg gacaatgacc atggcctgtt cttcgccgct ggtgctgtag acatactcga
     7321 ccccaggaat ctcccagatc agtttttcga gagggcgtac gacccggttt tcgacctgtg
     7381 ccggactggc acccggcata ccgacaaaga tgtcgaccat tggcacgaca atctggggct
     7441 cttcttcgcg tggaatgacc gccagcgaga agtagcccag cagcagcgaa gtaatgacga
     7501 agagtggggt gagtttggaa ttgacgaatt taccgacaaa acggccgctg aagttgagtt
     7561 ccataagagg ttcccccgtt taccgggatg tcgggcggcg gtcatactgg tcgccttcgt
     7621 tcaagccaga gagaatctcg acctcgtctc caatggtgcg tccggttttc accaggcgat
     7681 aaagactctt ctcgccagac tttacgaaca gtccgtcgag ttgtccccgg ctcacgacag
     7741 cgttcgtcgg caccaaaagt acctgctctt cgcctttcag taccgctcca cgtgcataca
     7801 tgccggtgcg gaaacgtgca tcgtccgtca gggtgaactt caccgtgaac gtgtgtgaca
     7861 tggggtcacc acccgggaca gtctcggcaa cggtcccgga aaacagttcc ggcccgagcg
     7921 catcaacccg cacggtcatg ctggctccgt cgggaagata actcaacaga cgctcaggca
     7981 cgatggcttc gaagcggatt cttccgaggt cttcgatgat catgagtggt acgccgggag
     8041 ccgcgagcat ccccggttcg actttgcgtg aaatgatacg cccggcgaac ggcgcgcgaa
     8101 cttcggccag ttctttcagt gtactgacct ttgaaatgcc tgcccgtgcc tgttcattgc
     8161 gggcgttgat ctgggcaagt ttggcctgca gactcgagat ctgcgacgcg gcctgcgtga
     8221 cctgagactt ggcctgggtc aggcgggtga aggcttgatc atgttcctgc tgggtggttg
     8281 acttcttgtc gtagagttct tttattcggg catacgagac ttctgccagt tgcagttggc
     8341 tctcggcggc ttctttggcc gcctgagccg tgttgatcgt gcttctgatc tcggcgatgg
     8401 ccgcctgagc ttctgccagt gtggccttgg cgccggccag atcgctgccg atctccttgg
     8461 catcaatgcg taccagcagc tcatgagccg ctacggtctc tccttcacgc ttcaagactt
     8521 cgacaacgtt tcccatcact ttgctcgaca acgtggtgac cgtggtgggt tgcagaatgc
     8581 cgacgacttc ctcgctgacg ggaatggttt tgcgtgccgc ggtagtccat tcgggagtgc
     8641 tcccttgagt gaaaccacca gaggcgaatg tcagaaccgc tgcgatggaa aacatcgcca
     8701 ggcgcaggaa cgaacgacgt ttgaatgggt gatgatgatt catggttctt ctcctcgacc
     8761 ggctgattgg cccggtcatt tggtctgtga cggcatttcg acagaggcgg cttcgggttg
     8821 cagaatgtca cacttttcaa ccgtcagatc gcccagagcg agcttcaggc gggcacggct
     8881 gatcgaatag tcatacagag cgttcaaatg gttcatcttg tgtgagagca gcgaagtctc
     8941 ggctcccagc agttcgctga cgatggcgag gccggcgacg taacgatctc gcagaatgcg
     9001 cagcgactct tcgctctggc caatggcctc acggctgacg cgaatgcgtt cgagggcgtt
     9061 attgatgttg gatatcggtt cccgctctga gggacgaagc gatacgacga agctgaaaac
//
LOCUS       DAORHD010000020         9080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7081_length_9080_cov_4.161219, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000020 DAORHD010000000
VERSION     DAORHD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7081_length_9080_cov_4.161219"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="PKO06_00950"
     CDS             complement(<1..1130)
                     /locus_tag="PKO06_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV68234.1"
                     /translation="MNDSLELFVFLVLLGLSAFFAASETAFFTLSPLRLKKLEEEGDT
                     CASEVLKLLERRQQLLTTLLLGSTLANVTATAMATHWLLQAVQRQADQLPALLSQNPV
                     IIGVAAAALGMTLLLVVGGEIIPRLIAQNSAQKTACRLVRPVRVCTLLFMPLTWVLMR
                     LVQPLTPSSSGTPTSTGTGASFDEIDSYFSLGEEVGIIERDEKEMISSVFEFGDTLVR
                     EVMVPRPDIVSVPITMKLDDLLKLVREDGHSRFPVYEASLDKILGVLYVKDLLMRYDE
                     IRASFDLHKLLRSVYFVPETKKLDDLLREFQKRKSHMAMVVDEFGGVSGLVTIEDLLE
                     EIVGEIVDEYDLDEQAQFVRVNDHTFVVDARMSISDLETELQ"
     gene            complement(1229..1756)
                     /gene="ybeY"
                     /locus_tag="PKO06_00955"
     CDS             complement(1229..1756)
                     /gene="ybeY"
                     /locus_tag="PKO06_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNV68235.1"
                     /translation="MEVLISNRQKKVKLNLKQIRLLAEEILRFEGVPENAELSLMFCD
                     DDEMQKLNHQYMGKDRPTDVLSFPLEEDDFAGDVRLLGDVVIDAETAIRQADKLGHSI
                     GLEIAFLLTHGILHLLRYDHMTRAEMARMKEREETICRHLSDKKLLKGLGSGSSGKAP
                     LIKRSVTPPRKPDAH"
     gene            complement(1719..3920)
                     /locus_tag="PKO06_00960"
     CDS             complement(1719..3920)
                     /locus_tag="PKO06_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNV68236.1"
                     /translation="MIERPGSYLNTVFEHLEIPEKNRRTFLIHVGVFFALVVGILYFD
                     PSGFNPEVSEGSLCRKAIVSPKNISFVDERKTDEIRRLEEEKVEPIVTHIENSEQEVM
                     AKLERFLTKANQLYNDWKDKELTEWTDTVVASYIPVDRLLEPESLKELVALSRTDFDR
                     VRTNSRKILQNFAQRTTTARNLETLKASVAKEVRLPNEWTTKLVVETVRNSLVINAIE
                     DEKLTREKRELARESVTPVKRSFTKGQKIIDQGVIITADDIYVLRAIEKQIQKNRLLG
                     FVGNLLLSGLLVGALLAHLRLTRHSLLKDLGLFRLQGGLLLVTLFLCRFVYSLGSALD
                     QPGLAILMSTLPSTGLLTAVLIDGQLALFQQMLLGILLFVISESNARFAIVSLIGSIT
                     GVLFWLGSSRTANLRGNLGWTGLKIGAVNAIAMNALMMLDVESSSIINVQEMLMAAAF
                     GIGNGIFTGILVNGTLPYLENAFALASGSRLLELTDLSQPLLRRMAEEAPGTYQHSIA
                     VATLSEAAAAEINADALLAKIGGYFHDIGKLKRPMYFAENQKKGENQHDHLTPYMSSL
                     ILVGHIRDGIDLGREYGLPESVVNFIGQHHGTSLISYFYDQAKNSTTDGQVSQDRFRY
                     GGPKPQTKETAIVMLADSVEASARTLPQHTHNRVEALVKKIVRGKMEDDQQLDQSELT
                     LKDIETIEQTFVKVILSMYHGRIEYPGKYANQPVEKGDTDGGSHQQPPKEG"
     gene            complement(3917..4960)
                     /locus_tag="PKO06_00965"
     CDS             complement(3917..4960)
                     /locus_tag="PKO06_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV68237.1"
                     /translation="MTQHTEVQIELSEELSRYLHAPGRKPSGLELIRGLPGLQAHARG
                     NRLSLSGTEDSLARCRRFLTILADRLASGQDLDPQELGHLFAEFAGPQSQELHQDRQT
                     GFHDILVTHSGRKVRPKTQRQQEYVEAIRTRDAVISIGPAGTGKTYLAVAMAVKALRD
                     KQVSRIILSRPTIEAGEKLGFLPGDLLEKVDPHFRPLYDALHEFLGISRFQQLLRQGV
                     IEITPLAYMRGRTFNEAFIVLDEAQNTTVPQMRMFLTRMGYGSRVVVTGDRTQIDLPN
                     RRDSSLLTLPEVLHDVERIGFITLEERDVVRHELVRDIIRAYEAYYGDTNAEGSETAR
                     QKRSRPGSEDTTA"
     gene            complement(4957..5802)
                     /locus_tag="PKO06_00970"
     CDS             complement(4957..5802)
                     /locus_tag="PKO06_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV68238.1"
                     /translation="MKKSLPGHILKAFRSIERFIPVVDLIIELVDARLPQASRIEGLV
                     RKLDKPSLIVLGKADLADPEATRTWIEHHERVGQPALALNAKQAASVKQLSSRIRQVA
                     TAVSTRLSKCRRLMVIGIPNVGKSTLINALAGRKAARVANLPGVTRSIQWVKLHGELE
                     LLDLPGVLDFRLLRQGEILRLINTLPGQTEDIEQDAVHLLDTLRRCGKAALVPDFSHN
                     TGSTRDFLPLYAAAMNFRIGGGAPDLNRAATDLIKRFQSGGFGRVTLETPDALLRAPE
                     MESAP"
     gene            complement(5943..6563)
                     /gene="lepB"
                     /locus_tag="PKO06_00975"
                     /pseudo
     CDS             complement(5943..6563)
                     /gene="lepB"
                     /locus_tag="PKO06_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            complement(6903..7271)
                     /gene="rplS"
                     /locus_tag="PKO06_00980"
     CDS             complement(6903..7271)
                     /gene="rplS"
                     /locus_tag="PKO06_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV68239.1"
                     /translation="MNPIIESIEREYQKNPKAVPAFRPGDTLRISLKVKEGDKERLQA
                     FEGVVIAKAYSGLRANFTVRKISYGVGVERKFMLHSPSIADIQVMRRGVVARSKLYYL
                     RGRVGKKTRVKDKYIAKKEA"
     gene            complement(7291..8046)
                     /gene="trmD"
                     /locus_tag="PKO06_00985"
     CDS             complement(7291..8046)
                     /gene="trmD"
                     /locus_tag="PKO06_00985"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV68240.1"
                     /translation="MIIDLVTLIPGIFDRVFDESILGSAKESGLLEIRCHNFREFSTD
                     RHKTVDDYPFGGGPGMLIKPAPLAECLRSVRRQGPAGPVIGLTPQGVPLNQGIVRRLA
                     QYPRLIIVCGRYEGFDERLLPEFDLELSVGDYVVTGGEFPAMTLVDAVSRMIPGTVGR
                     TASVEQDSFFTGRLDHPQYTRPAEWEGQSIPEVLRQGHHEKIETWRRNQALLRTATRR
                     PDVFTRLTVERDEAVAFQQVFGPSNTSTHHSGK"
     gene            complement(8043..8705)
                     /locus_tag="PKO06_00990"
     CDS             complement(8043..8705)
                     /locus_tag="PKO06_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68241.1"
                     /translation="MWLTRHHPELRTAKRPLSEKAGKPARTATVTPAPARPPIVRGVE
                     LSEADLIRVGQAKKPREDGWIRLKMHTDHPERFDVGSELLAEIEPGRGVPVKVIDSRD
                     SGPADEVEIRIAEIPTGTPCVVHPSGRLFIEKQQRKPAPRGTFYPDELEGMTVEDCKG
                     ELVGTVVHLEADVPAPYLVVKSDQYGELLIPYHRQVLREVSREKRRVKLVGTAAEQVP
                     ES"
     gene            complement(8715..8957)
                     /locus_tag="PKO06_00995"
     CDS             complement(8715..8957)
                     /locus_tag="PKO06_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNV68242.1"
                     /translation="MELDSIQQIVLNIVQTLVQYPDQVNIKPIEGESTIVFEVQVHPE
                     DAGKVIGKKGRTINALRTILRASTLLEGKKVMMELV"
BASE COUNT         1831 a         2603 c         2714 g         1932 t
ORIGIN      
        1 atctgcagtt cggtctcgag gtcgctgatg ctcatgcgcg catcgaccac gaacgtgtgg
       61 tcattgaccc gtacgaattg ggcttgttca tccagatcgt actcgtcgac aatctcgccg
      121 acgatctctt cgagcaggtc ttcgatggtc accaggcctg aaacgccacc gaactcgtcg
      181 acgaccatgg ccatgtgact tttccgtttc tgaaactcgc gcaggagatc gtcgagcttc
      241 ttggtttcag gcacgaaata cacggatcgc agcaacttgt gcaggtcgaa gctggcgcga
      301 atttcgtcat accgcatcag caggtctttt acgtagagga ctccgagaat cttgtcgagc
      361 gatgcttcat agacgggaaa ccgtgaatgt ccatcttctc gaaccagctt caataagtcg
      421 tcaagcttca ttgtaattgg tactgagacg atgtcgggtc ggggcaccat gacctcgcgt
      481 accagtgtgt cgccaaactc gaagacggat gagatcattt ccttttcgtc gcgttcgatg
      541 ataccgactt cttcaccaag agaaaagtac gagtcgattt cgtcgaaaga cgctccagtt
      601 ccggttgatg ttggggttcc gctgctggat ggggtgagcg gctgcaccag gcgcatcagc
      661 acccaggtca gtggcatgaa gagaagtgtg catacgcgca caggacgcac cagccgacag
      721 gcggtttttt gcgcggagtt ctgagcgatc aatcggggaa tgatctcgcc gccgacgacc
      781 agtaacagcg tcattcccag ggcagccgcg gcaacaccga tgatcaccgg attctgtgac
      841 aagagtgctg gcaactgatc agcctgtcgc tgcacggctt gcagcagcca gtgggttgcc
      901 atggccgtcg ccgtcacatt cgcgagcgtg ctgccgagca gcagggtcgt gagcaactgt
      961 tgacgccgtt cgagcagttt caggacctcg ctggcacagg tgtcaccttc ctcttcgagc
     1021 ttcttcagac gcagcggaga aagggtgaaa aacgcggtct cgctggcggc gaaaaaggcc
     1081 gagaggccca gcaaaaccag aaacacaaaa agttcgagcg agtcgttcat cgggcgcgaa
     1141 cctcatcggc agatcgaact gtgcctgtgc tcaacagcac acgcgtttcg tcgaggttcg
     1201 cagaggggga atcggggctc acgtgcagtc agtgggcgtc cggtttccgt gggggagtca
     1261 ccgaacgttt gatcaggggc gctttgccgc tgctgccgct gcccaggccc ttcagcagtt
     1321 ttttgtcaga gagatgacgg cagatcgtct cttctcgttc cttcatgcgt gccatttcag
     1381 cgcgcgtcat atggtcgtag cgcagcagat gcagaatgcc atgcgtcaag agaaaggcaa
     1441 tttcgagacc gattgaatgc cccagcttgt ctgcctgccg gatcgccgtt tcggcatcga
     1501 tcaccacgtc gccgagcaga cgcacgtcac ccgcaaagtc gtcttcctcg agcggaaacg
     1561 aaagcacgtc ggtcgggcgg tcttttccca tgtactgatg attgagtttc tgcatctcgt
     1621 cgtcatcgca gaacatcagc gaaagctcgg cgttttccgg aaccccttcg aaacgcagga
     1681 tctcctcggc cagaaggcga atctgcttca gattcagctt aaccttcttt tggcggttgc
     1741 tgatgagaac ctccatccgt gtctcccttt tctaccggtt gattggcata cttgcccggg
     1801 tattcaattc tgccatgata catggaaagg atgaccttga caaaggtctg ctcgatcgtc
     1861 tcgatgtcct tcagcgtcaa ctcgctctgg tcgagctgct gatcgtcttc catctttccc
     1921 cgtacgattt ttttcaccag ggcttcaaca cgattgtgcg tgtgctgcgg caaggtgcgg
     1981 gctgaggctt cgacggaatc ggccagcatg acgatagcgg tttccttcgt ctgtggcttg
     2041 ggcccaccat agcgaaaacg gtcctggctg acctgaccgt ccgtggtgct gttcttggcc
     2101 tggtcgtaaa agtagctgat caggctggtg ccgtggtgct gaccgataaa attcacgacc
     2161 gattcaggca gaccatactc tcgacccaga tcgataccgt cgcgaatgtg tccgaccagg
     2221 atcaaactcg acatgtacgg cgtcagatgg tcgtgctggt tctcgccttt cttctgattc
     2281 tccgcgaaat acattggtct cttcagcttg ccgatgtcgt gaaagtaccc gccgattttc
     2341 gccagaagcg catcggcatt gatttctgcg gctgccgctt ctgagagcgt ggcgaccgcg
     2401 atgctgtgct gatacgttcc gggggcttct tcggccatgc gacgcagcag cggttgagac
     2461 agatcggtca attcgagcag gcgagagccc gaagccagcg cgaaagcgtt ttccagatag
     2521 ggcagagtgc cgttcaccag aatgccggtg aagatgccgt tgccgatacc gaaggcagct
     2581 gccatcagca tttcctgcac gttgatgatg ctcgaacttt cgacatccag catcatcaga
     2641 gcgttcatgg caatggcgtt gaccgcgccg atcttgagac cggtccagcc gaggttccct
     2701 cgcaggttgg cggtacggct tgaacccagc cagaacagga cgcccgtaat gctgccgatc
     2761 aggctgacaa tggcaaagcg cgcgttcgac tcagagatga cgaacagcag aattcccaac
     2821 aacatctgct ggaagagtgc cagttgtccg tcgatgagaa ccgcggtcag aagcccggtc
     2881 gacgggagtg tcgacatcag aatggccagc cccggctggt caagggcgct gcccagcgaa
     2941 tacacgaagc gacacaggaa cagcgtgacc agcagcagac cgccctgcag tcgaaacaac
     3001 cccaggtctt tcagcaggct gtgccgggtg agacgcagat gagccagcag agcgccgaca
     3061 agcagccccg acagcagtag attgccgacg aagcccagca ggcggttttt ctgaatctgt
     3121 ttctcgatgg cgcgcagcac gtagatgtcg tcagccgtaa tgatcacgcc ctgatcgatg
     3181 atcttctgcc ctttggtaaa cgatcgtttg accggggtca ccgactcgcg ggccagttcg
     3241 cgtttctcgc gcgtcagttt ctcgtcttcg atggcgttga tgaccaggct gttccgcacg
     3301 gtttccacga ccagtttcgt ggtccactcg ttgggcaacc gcacttcctt ggccacagag
     3361 gccttcaggg tttccagatt gcgagcggtg gtcgtgcgct gggcgaagtt ctgcagaatc
     3421 ttgcgcgaat tcgtgcgcac ccggtcgaag tcggtgcgag agagcgccac cagctctttc
     3481 agagactcgg gttcgagcag gcgatcaacc ggaatgtagc tggcgacgac cgtgtcggtc
     3541 cattcggtca gctccttgtc tttccagtca ttgtagagct ggtttgcctt ggtcagaaag
     3601 cgctccagct tcgccatgac ttcctgttcg gaattctcga tgtgagtgac gatcggttcg
     3661 accttctctt cttcgagtcg ccgtatctcg tcagttttgc gttcatcgac gaagctgatg
     3721 ttcttggggc tgacgatcgc cttgcggcag agggaaccct cggaaacctc tggattgaag
     3781 ccactcggat cgaaatacag aatgcccacc accagcgcga aaaacacccc gacgtgaatc
     3841 agaaaggtgc gtcggttttt ctcgggaatc tcgagatgct cgaagacggt attgaggtag
     3901 gatcccgggc gctcaatcac gcggttgtgt cctccgatcc cgggcgggag cgcttctgcc
     3961 gcgcggtctc tgaaccctcg gcgttcgtat cgccgtagta ggcttcgtaa gccctgatga
     4021 tgtcgcgaac cagctcatgg cgcaccacgt cacgctcctc gagggtgatg aacccgatgc
     4081 gttcgacgtc gtgcaacact tcgggaaggg tgagcaatga tgaatcgcgg cggttcggca
     4141 ggtcgatctg cgttcggtca cccgtgacca ccacgcgtga gccatatccc atgcgggtga
     4201 ggaacatgcg catctgcggc acggtggtat tctgggcttc gtcgagaacg atgaatgctt
     4261 cgttgaacgt gcgaccgcgc atgtacgcaa gcggagtgat ctcgatgact ccctggcgca
     4321 gcagttgctg aaatcgtgaa atgccgagga attcatgcaa ggcatcatac agcggacgaa
     4381 agtgcggatc aaccttctcc agcagatcgc cgggcagaaa gccaagtttt tcgccggcct
     4441 cgatggtggg tcgcgacaga atgatgcggg aaacctgctt gtcacgaaga gctttgacgg
     4501 ccatcgccac ggccagatac gttttcccgg tgccggccgg gccgatcgaa atgacggcat
     4561 cacgcgttct gatcgcttcc acgtactcct gctgccgctg ggttttcggt cgaaccttgc
     4621 gcccggaatg cgtcacgaga atgtcgtgaa agcctgtctg acggtcctga tgcagctcct
     4681 ggctctgcgg tccggcgaac tccgcgaata aatgtccgag ttcctgcggg tcgaggtcct
     4741 gtcctgaagc caggcgatcg gcaagaatgg tgagaaatcg gcggcagcgt gccagactgt
     4801 cttcggtgcc gctcaacgac aggcgattcc cgcgggcatg cgcctgcaga cccggcagac
     4861 cgcgaatcag ctcgaggccg ctcggtttgc gacctggtgc atgcagatag cgagaaagct
     4921 cttcgctcag ttcgatctgg acttcggtgt gctgcgtcat ggtgcgctct ccatctccgg
     4981 cgctcggagc agtgcgtcag gagtttccag agtgacgcgg ccaaagccac cgctctgaaa
     5041 tcgtttgatc aggtcggtgg ccgcacggtt cagatcggga gctcccccgc cgatgcggaa
     5101 attcatggcg gcagcataga gcggcagaaa gtctcgggtt gatccggtgt tgtgcgagaa
     5161 gtccggaacc agcgccgctt tcccgcaccg tctcagggtg tcgagcagat ggacggcatc
     5221 ttgttcgatg tcctcggttt gtccggggag agtgttgatc aaacgcagta tttctccctg
     5281 tcgcaggagt ctgaaatcga gaacgccagg gagatcgagc agttcgagct ccccgtgcag
     5341 tttgacccat tgaatgctcc gggtcacccc gggaagattg gcaacgcgcg cagccttgcg
     5401 ccccgccaga gcgttgatca gcgtcgattt gccgacattg ggaatgccga tgaccatgag
     5461 tcgccggcac ttcgacaaac gcgtggaaac cgcggtcgcc acctgccgaa tgcgtgacga
     5521 aagctgcttg accgacgctg cctgcttggc attcaaagcc agggctggtt gtccgacgcg
     5581 ctcgtggtgt tcgatccagg tgcgtgtggc ttcagggtcg gccaggtcag ctttccctaa
     5641 aacgatcagt gacggcttgt cgagtttccg taccagccct tcgatgcgcg atgcctgtgg
     5701 cagacgcgcg tcgacgagtt cgataatcag atcgacaacc ggaatgaacc gctcgatcga
     5761 gcggaatgcc ttgagaatgt gcccgggaag agactttttc actggtccga agacgatggt
     5821 tcgccgtctt cggcgccgcg ggaaggagtg gcacgaggct gcacgggagc gaacgggccg
     5881 ggaaactcaa aagtcgcagg gtcgccggat ttgcgtactt gcagtggatg agaatagctg
     5941 cggatcggcc cgatgcgctt gaacggccag tacaaaaaca cggcctgtcc tttcagattc
     6001 gcgagtggca tgggccccca gtaccggctg tcggcactgt tgttccgatt gtcgcccaga
     6061 acgaagaggc tgtccagggg gacggtggtc tccatatcct gccagggcgg ttccgcgata
     6121 tacggctctt cgagcgccgt gtcattgacg aagaccgtgc cctcgatgat gcgaatgcga
     6181 tcacccggca aaccgatgac ccgcttgatg aaatcgcggg gggcggcttt cgggtccggg
     6241 tctggatact tgaacacgac gatatcgccg cgtttgggaa accgcaacca gagcttgggc
     6301 aaccatttca gacgcccgtc gagcgagaga tcggcgacat aggcaaactt gttgacgaga
     6361 atgcgatcgc cagactgggt gcgctgacgt gtcaccgggt cttgcatctc gcccagcagc
     6421 gtcggttgca tgctgccggt gggaatccag aaggcttgca ccacgagact ccgaatgatc
     6481 agcgccagca cgaaggcaat gaagacggtc tcattccatt cgcgaaacga ggatttatag
     6541 aaaagacgat aacgaggtga aatctctttc accccgtccg gatccgtcag tcgaacaaaa
     6601 aactcgttcg gtccgttggt cagaatcagc tcagcgccga tctgatgccg ttcaggcttg
     6661 atgagaaccg tcgagatggc acccgatcgc gagacgcgac acaactcaag ggtagccgcc
     6721 gatgatggcg tcatcgagag atgaccagaa aaccagagcg tatgttcgct cagcacctct
     6781 tcgggtgaga ggccttcgag cggaacaatg gttccctgac cgacgcggat cgtcggattg
     6841 cgcaccatcg gcgactgccc ttgagcagtc gtggaaggaa cgtcggcagt caacggctgg
     6901 ccttacgctt ccttcttggc gatgtacttg tccttcacgc gggtcttctt gccgacgcga
     6961 ccgcgcagat agtacagctt cgagcgggcg accacaccgc ggcgcatgac ctggatgtcg
     7021 gcaatgctgg gcgaatgcag catgaacttg cgctcgacgc cgacgccgta agagatcttg
     7081 cgaaccgtga agttggcgcg cagacccgag taagccttgg cgatgaccac gccttcaaag
     7141 gcctgaaggc gctctttatc gccttcctta accttgaggg agatgcgaag agtgtcaccg
     7201 ggccggaatg cgggcacggc cttcgggttc ttctgatact cgcgctcaat gctttcgatg
     7261 atggggttca tatcgtgact ccttcctgag ttacttgcct gaatgatgag tgctggtgtt
     7321 cgacggaccg aacacctgtt gaaacgccac ggcttcgtcg cgttcgacgg tcagccgggt
     7381 aaagacatcc ggacggcggg tggccgtgcg caacagcgct tggtttcgcc gccaggtttc
     7441 gattttttcg tgatgcccct gccgcaatac ctcgggaatg ctctggcctt cccactcggc
     7501 ggggcgggta tactgcgggt ggtcgagcct gccggtgaag aaggaatcct gctccaccga
     7561 ggcggtgcgg ccgaccgtac ctggaatcat gcgggagacg gcgtcgacca gcgtcatggc
     7621 gggaaactct ccgcccgtga cgacgtagtc gcccacggaa agttccagat caaactctgg
     7681 cagcagtcgc tcgtcaaacc cttcataacg cccacagaca atgatcagac gcggatactg
     7741 cgccagacgt cggacaatcc cctgattcag cggcacaccc tgcggggtca gcccgatgac
     7801 cggacccgcc ggaccctgtc ggcgcaccga gcgcagacac tcggccagcg gtgccggttt
     7861 gatcagcatg cccggaccgc cgccgaacgg gtaatcgtcg accgtcttgt gccgatcggt
     7921 actgaattcc cgaaagttgt ggcagcgaat ctcgagcaag cccgactctt tcgccgaacc
     7981 gagaatgctc tcgtcgaaca cccgatcgaa gatgccggga atcagcgtga ccagatcgat
     8041 gatcatgact cgggcacctg ctcagcggcc gttccgacca gcttcacccg ccgcttctcg
     8101 cgcgaaacct cgcgcagaac ctgtcggtga tacggaatca gcagttcgcc gtactggtcg
     8161 ctcttcacga ccagataggg cgcgggaaca tcggcttcga gatggacgac ggtgccgacc
     8221 aactcaccct tacagtcttc gacggtcatg ccttcgagtt catcgggata aaacgtgccg
     8281 cgcggagcgg gcttgcgctg ctgcttctcg atgaacaagc gaccagacgg atgaaccaca
     8341 cacggtgtcc cagtggggat ctcggcgata cggatctcga cctcgtcagc cggacccgaa
     8401 tctcgagagt caataacctt cacgggaaca ccacgacctg gttcgatttc ggccagcagt
     8461 tcactgccga cgtcgaagcg ctcgggatga tccgtgtgca tcttcaggcg aatccaacca
     8521 tcttcgcgag gcttcttggc ttgtccgact ctgattaaat cagcttcaga cagttcgacg
     8581 cctcggacaa tcggtggacg tgccggtgcc ggcgtcaccg tcgcggtgcg tgccggcttc
     8641 ccggccttct cggagagagg ccgtttggcg gtgcgcagct cagggtgatg acgggtcagc
     8701 cacatacgac gtcgtcagac cagctccatc atgactttct tgccttccag cagcgtcgat
     8761 gcgcgcagga tcgtgcgcag ggcgttgatg gtgcggcctt tcttgccgat gactttgccg
     8821 gcgtcttccg ggtgtacctg cacctcgaag acgatggtgc tctcgccttc gatcggtttg
     8881 atgttgacct ggtcgggata ctgaaccagc gtctgaacga tgttcagaac gatctgttga
     8941 atggaatcga gttccaaggt tcagttcctg gtcaggactt cttgacccag gtgccggtgt
     9001 tggtgcggat aatgcccttc tcgcgcagga gaggaacaac cgcgtccgtc ggctgggcgc
     9061 cattctgcag gtgcttgagg
//
LOCUS       DAORHD010000021         9067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7092_length_9067_cov_3.642255, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000021 DAORHD010000000
VERSION     DAORHD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_9067_cov_3.642255"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..847
                     /locus_tag="PKO06_01000"
     CDS             89..847
                     /locus_tag="PKO06_01000"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV68243.1"
                     /translation="MATRACQKTPENPIDRLKHERRGVTYADLLLKPDDELWEVIDGV
                     PYLLATPTIEHQEVSVKLSRFLDESFEGQPCRVFHAPTGVFLPKKNETLDETTNFVIP
                     DLIVVCDKAKLESRGCRGAPDLVIEILSPSSGARDQLVKLNFYEKHGVPEFWIVDPTL
                     KTVAVFRLEAGAKRYGRPIYHDRTKRIRSPRFPGLALDLARVFPPPEKAVREPSATFS
                     TARTPRVRKAKPKTLAPRPPARKPSTPKRSSARA"
     gene            913..3702
                     /locus_tag="PKO06_01005"
     CDS             913..3702
                     /locus_tag="PKO06_01005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV68244.1"
                     /translation="MPITSNTPVPERSSTFVSALIEHNSVGLSPEGRLYLCHDPSEAH
                     GLDQPIVTRLHESFERGSGHGLFLLGAREARTLLPASLRFWREFAGLFVRQLVRIEDL
                     ESARHDPQVAWPGEEVLRWLETAPPFRGAEYLREPTLRTLWEHMRRAFTEILDAWAGT
                     VDEYFQTVNPLWNMLGRVCFHLADRKDDDTRPFAFLATYTSGISQHGKPAHLPLSEAL
                     KEYAGARDRTALIRLLATVHRASASSAFLKGIVDSRRIFHPLTWTVAEAYQFLREIPL
                     YEAAGIVCRVPDWWKPGSRSTLAASLEIGSKPASDLGWRGMLDYSLQLCLDGEPLTAE
                     EWRAIKNRQDKLVYLRGRWIEIDTEELNQVLTHFERWQQGRRGTGLSFAEAMRLVSGA
                     NLDQDSPLPVGTEAAARVIPGAWLSSIIERLRDPAALTAVEPGPDLQGTLRPYQARGL
                     HWLWFLLELGLGPCLADDMGLGKTIQVLALLLASKRTRSLSSGSASDGASESRPALLV
                     LPASLLSNWEAERQKFAPSLQALCAHPSVMPSANIGKLRSEDLQNVDMVLTTYGTLVR
                     QQRLREMNWSLVILDEAQAIKNPDTRQARAVRQLNADRRLALTGTPVENRLGDLWSIF
                     DFLNPGLLGSAGEFKKFLETLETSAGTSYAPLRRLVRPYILRRLKTDPDVAPELPPKT
                     EVKTSCSLVKEQAVLYETAVNDLVKDLETVDPLKRRGLILAYLLRFKQICNHPSQWLK
                     DDNYDPAHSGKFARLREICEEIASRGEKVLVFTQFREMTGPINGFLRSLFGNDGLVMH
                     GSTPIKERGTLVNRFQEDPLISYLVLSLKVGGVGLNLTAANHVIHFDRWWNPAVENQA
                     TDRAFRIGQKKNVVVHPFVCRGTLEEKIDALLERKKALANELLGQEEYPLITELSDRE
                     LLSLVRLDVRSALGD"
     gene            3770..5161
                     /locus_tag="PKO06_01010"
     CDS             3770..5161
                     /locus_tag="PKO06_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HNV68245.1"
                     /translation="MMARSASSRSHTSRASTRSSRHSYGRYGYGGYGYGGFPPYVSVA
                     EKKQRALEAAQKLVKAGRKLHPIEVVGRDVCHTWWGLSWCRNLEHFGDYENRLPRGRS
                     YVRTGSVIDLQIAHGRVKGLVQGNDLYEVEIRIKPLAEDRKNELCERCASDIGSTIDL
                     LSGKLDRNVMNILAEPKRGLFPEPRDIEFTCSCPDYASVCKHVAAVLYGIGVRFDTAP
                     ELFFKLRGIDPDEFASSATAGLGSTLPPADPKETLVNSSIQELGAIFGIDFAPTGENN
                     ADTKIKLAARGGASAVPMPAVSTPGVSSAEAFVESLFDPISGKPIDVDADAEGEKKSR
                     GQRKPVRQKARQSVMVPTAVTKKSAQTISKRRKLTTAVTGKNRSGRSADKTIRHKKQS
                     ADSSLVNRCQPRQMLKLSELRELGIPYSRVQKALLDGHLEITAWRGEYRCTRTTTNWL
                     KNFVRVSISEIAD"
     gene            5186..5611
                     /locus_tag="PKO06_01015"
     CDS             5186..5611
                     /locus_tag="PKO06_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNV68246.1"
                     /translation="MADLDIRWKQRLSHYQQALLQLTQAVELSRSRALSDLEKQGLIQ
                     AFEFTHELAWNVMKDYFVDQGNPNITGSRDAFREAFQKGLIQEGENWMNMIKSRNLAS
                     HTHNRKTAEELVQAILHTYHNLFRQFAETMQKLADARHS"
     gene            5595..5924
                     /locus_tag="PKO06_01020"
     CDS             5595..5924
                     /locus_tag="PKO06_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV68247.1"
                     /translation="MPDIPETTRFGLREETIRLIQQVFASHPHIEQAILYGSRAKGTH
                     RDASDIDLTLVGAMLTLNDSCAIAQELDDLLLPWKIDLSLRHTIDNPALLDHIERVGR
                     EFYRRPA"
     gene            complement(5994..6584)
                     /locus_tag="PKO06_01025"
     CDS             complement(5994..6584)
                     /locus_tag="PKO06_01025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV68248.1"
                     /translation="MANDFFVHESSYVDDGVVIGKDTKIWHFCHILGKTRIGEKCSFG
                     QNCVVGPNVVIGNNVKVQNNVSIYDGVELEDDVFCGPSMVFTNVINPRSHVIRKHEYK
                     KTLIKRGASIGANATVVCGHTLGEFCFIGAGSVVTKDVPAYALIVGNPGRITGWMCHC
                     GVKLVSGKDLKESATHTCAVCNSAYTVKGGKLTPAC"
     gene            complement(6675..7808)
                     /gene="wecB"
                     /locus_tag="PKO06_01030"
     CDS             complement(6675..7808)
                     /gene="wecB"
                     /locus_tag="PKO06_01030"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018866768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNV68249.1"
                     /translation="MKLMTVLGARPQFIKAAVVSRALRSAAGGGEPIRELIVHTGQHY
                     DANMSQVFFDEMDIPRPTHSLNIGSGSHGAQTGAMLAAIEKVALDEKPDWVLVYGDTN
                     STLAGALVAAKLHIPVAHVEAGLRSFNRRMPEEINRIMTDHVSTALFCPTQTAVDNLR
                     HEGLPSSRLPKVQVVYCGDVMYDAALFYGTRAPVLTSVAGLSLKPKAFILATIHRAET
                     TDDAATLAGVVELLTKIGSEFAPVVWPIHPRTRHLLEKRGDLSRLLERGGQRLKLIDP
                     VGYLEMVALEKAARLIVTDSGGVQKEAYFHHTPCVTLRTETEWVELVEAGWNQVVGTD
                     PTNVIRAVQAAWNENWLSKSRPELYGRGNAGELIAQWLRDHRA"
     gene            7944..8723
                     /locus_tag="PKO06_01035"
     CDS             7944..8723
                     /locus_tag="PKO06_01035"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV68250.1"
                     /translation="MTGSQQNRSSSSTALSTSASRCGKKPCTYADLQKMPADELWELI
                     DGVPHAMTVPSADHQFVSVQLTRQLANFFHRSPCQVFHAPYGVFLPKKNESIEKTTNL
                     VIPDLVVVCDEKKRTPQGCRGAPDLVIEILSPSTAGRDRVKKLNLYERFGVKEFWIIS
                     LDSAAVMVFRLDADGRYARPVQFYWSDKLTTPLFPALEVDLSEVFPKFVDTEDPETPD
                     DSPPPSTPSDAPAPRVREDAKPFAAPAKRRSGQRKSATKRR"
     gene            8781..9035
                     /locus_tag="PKO06_01040"
     CDS             8781..9035
                     /locus_tag="PKO06_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNV68251.1"
                     /translation="MQAVNYSTARNNLKSIIEDIDSNGKEYLVVTKEDRQVVIISYDE
                     YQSMKETLYLLSTPANSERLLWSIGQIEKSKFQRYKLIDP"
BASE COUNT         1924 a         2809 c         2470 g         1864 t
ORIGIN      
        1 gaaattgcaa cgctcgcgtg ccgcgcgact tatcggagga gagtgctgtg gtatgatggg
       61 gcaaacctca ctgtcggaga gtggacacat ggcaacacga gcttgtcaga aaacacctga
      121 gaacccgatc gatcggttga aacacgagcg ccgaggggtg acctacgccg atctcttgct
      181 caagccagac gatgagttgt gggaagttat cgacggggtg ccgtatctac tggcaacgcc
      241 aaccatagag caccaggaag tttcggtgaa gctctctcga tttcttgacg aatccttcga
      301 aggtcagccc tgccgagtgt tccatgcgcc aactggggtg tttcttccga agaaaaatga
      361 aacactcgac gagacgacca actttgtcat tccggatctc atcgtcgttt gcgacaaggc
      421 aaagctcgaa tcgcgcggct gtcgaggagc accggatctg gtcatcgaga ttctctcgcc
      481 ttcaagcggt gcgcgcgatc agctcgtcaa attgaacttc tacgagaaac acggcgttcc
      541 cgagttctgg atcgtcgacc ccaccctgaa aaccgtcgcg gtgtttcgcc tcgaagccgg
      601 cgccaaacgc tatggccggc caatctacca cgatcgtacc aagcgcatcc gatcgccccg
      661 gttccccgga ctcgcgctcg atctcgcacg tgtttttcca ccccctgaaa aagccgtgcg
      721 tgagccctcg gcgacgtttt cgaccgcccg cacccctcgc gtgcgcaagg caaagcccaa
      781 gacgctggca cctcgtccac ctgcgcgcaa gccatcgacc ccgaaacgat catccgctcg
      841 cgcatgaatg tgtgcttgcc gtcacctcgc cacgcaccac gagagaggcc agtcgaatga
      901 tcttttcaca ccgtgccaat cacctccaac accccggttc ctgagcggtc ttcgacgttt
      961 gtttcagctt tgatcgagca taacagcgtc gggttgagcc cggaaggtcg gttgtatcta
     1021 tgccatgacc cttccgaggc gcacgggctc gatcaaccca tcgtcacgcg cctgcatgag
     1081 tctttcgagc gcggctcagg acatggactt tttctgcttg gcgctcgcga ggcacgaacc
     1141 ctgcttccgg cttcattgcg cttctggcgc gagttcgcag gcctgttcgt gcggcaactg
     1201 gtccgtatcg aggacctcga atcggcacga catgatccgc aggttgcctg gcccggcgag
     1261 gaagttctgc gctggctcga gaccgctccg ccgtttcgcg gggccgagta tctgcgcgag
     1321 ccgaccctgc gcaccctgtg ggaacacatg cgacgggcct tcaccgaaat cctcgacgca
     1381 tgggccggca cggtcgacga gtattttcag acggtcaacc cactctggaa catgctcggg
     1441 cgagtctgct tccatctcgc cgaccgcaaa gacgacgata cacgaccctt cgcgtttctc
     1501 gccacctata cttcaggcat ctcacagcac ggaaagccag cccatctgcc gctcagcgaa
     1561 gctctcaagg agtacgccgg ggcgcgcgat cgcaccgctc tgattcgcct gctcgcgacc
     1621 gtccatcgtg ccagcgcgtc gagcgcgttt ctgaaaggca tcgtcgacag tcgccgcatt
     1681 ttccacccgc tcacctggac cgtcgctgaa gcgtaccagt ttctgcgcga gattccgctc
     1741 tacgaagcgg ccggcatcgt ctgtcgcgta cctgactggt ggaaaccagg ctcgcgctcg
     1801 acgcttgcgg cttccctcga gatcggcagc aagcccgcct cagatctcgg ctggcgaggc
     1861 atgctcgatt actctctgca gttgtgtctc gacggcgagc ccctgaccgc agaagaatgg
     1921 cgcgccatca agaaccgaca ggacaaactc gtctacctgc gcgggcgttg gatcgagatc
     1981 gacaccgaag aactgaatca ggtgctgacg catttcgagc gctggcaaca gggaaggcgc
     2041 ggtaccgggt tgtcgttcgc cgaagccatg cgcctggtct cgggtgccaa cctcgatcag
     2101 gattcgcccc ttccggtcgg taccgaggcc gcggcgcgcg tcattcccgg cgcgtggctg
     2161 tcatcgatca tcgagcgttt gcgcgacccg gccgctctta cagccgtcga gcccggccct
     2221 gacctgcaag gcactctccg cccctatcag gcccgcggac tgcactggct ctggtttctg
     2281 ctcgaactcg ggctcggccc ctgcctcgcg gacgacatgg gtctcggcaa aacgattcag
     2341 gtcctggctc tgctgttggc cagtaagcgt acccgttcac tctccagcgg ttcagcatct
     2401 gacggggctt ctgagtcaag gccggccttg ctggtgcttc cggcatctct gctgtcgaac
     2461 tgggaagccg agcggcagaa gttcgctccc agcttgcagg ctctctgcgc gcaccccagc
     2521 gtcatgcctt cggcgaacat cggcaaactg cgctccgaag atctgcaaaa cgtcgatatg
     2581 gtgctgacca cctacggcac tctcgtgcgc cagcaaaggc ttagagagat gaactggagt
     2641 ctcgtcatcc tggacgaagc tcaggcgatc aaaaaccccg atacgcgaca ggcacgagca
     2701 gtcagacaac tcaacgccga tcgccgcctc gctttgaccg gaactccggt cgagaaccga
     2761 ctcggcgatc tgtggtcaat cttcgatttc ctcaaccccg ggttgctcgg ctcagccggc
     2821 gagttcaaga agtttctcga aacgctcgaa accagcgccg gcacatcgta tgctcccctg
     2881 cgccgactgg tacgcccgta tattctgcgg cgtctgaaga ccgaccccga cgtcgcgccc
     2941 gaactgccgc ccaaaaccga ggtcaaaaca tcctgttcac tcgtgaaaga acaggccgtg
     3001 ctctatgaga cggcggtgaa cgatctggtc aaggatctcg aaaccgtcga tccgctcaag
     3061 cgacgcgggt tgattctcgc gtatcttctg cgtttcaagc agatctgcaa tcatccgtcg
     3121 cagtggctga aagacgataa ctacgatccg gcgcacagcg gcaagtttgc ccgactgcgc
     3181 gagatctgcg aggaaatcgc ctcacgcggg gaaaaagtgc tggtgttcac gcagtttcgc
     3241 gagatgaccg gcccgatcaa cggcttcctg cgctcgctct tcggcaacga cggcctggtc
     3301 atgcacggca gcactccgat caaggaacgc ggcactctgg taaatcgatt tcaagaggat
     3361 ccgctgattt cgtatctcgt actctcactc aaggtcggcg gggtcggtct caatctcacc
     3421 gccgcgaatc atgtgattca cttcgaccgc tggtggaacc cggccgtcga aaaccaggcc
     3481 accgatcgcg ctttccggat cgggcagaag aagaatgtgg tggttcaccc tttcgtctgt
     3541 cgcggcacgc tcgaagagaa gatcgacgca ctgctcgagc gcaagaaagc cctggccaac
     3601 gaactgctgg gacaggaaga atacccgttg atcacggaac tgagtgaccg tgaactgctc
     3661 tcgctggtac gacttgatgt gcgttcggct cttggcgact gagaaagcat ctgctaccat
     3721 gacgtcacac cggttcacct aagcgctcac tttcattttt cgaggcttca tgatggcacg
     3781 ttctgcatcg tcacgatcgc acacctcacg cgcttcaaca cgttcttctc gccactcgta
     3841 cggtcgctat ggatacggtg ggtacggata cggcgggttt cccccgtatg tgtcggtcgc
     3901 cgagaagaaa cagcgcgctc tcgaggccgc ccaaaagctc gtgaaagccg gccggaaact
     3961 gcacccgatc gaagtcgtcg ggcgcgacgt ctgtcatacc tggtggggcc tttcgtggtg
     4021 ccgcaacctc gaacatttcg gcgactacga gaaccgcctg ccgcggggcc gttcgtatgt
     4081 tcgcaccgga tcggtcatcg atcttcagat tgctcacggc cgcgtgaagg ggctggtgca
     4141 agggaacgac ttgtacgaag tcgagattcg catcaagcca ttggcggaag atcgcaagaa
     4201 cgaactatgc gaacgctgtg cttcagacat cggctcaacc atcgatctgc tcagcggcaa
     4261 actggaccgc aacgtcatga acattctggc tgagccgaag cgcgggttgt ttccagagcc
     4321 acgagacatc gagttcacgt gttcctgccc cgattatgcc tcggtttgca agcatgtcgc
     4381 cgctgtgctc tacggcatcg gggtccgttt tgacacagcg ccggagctgt tcttcaaact
     4441 gcgcggcatc gaccccgatg agttcgcatc atcggcgaca gccgggctcg gctcgactct
     4501 gccccccgcc gacccaaaag aaactctggt gaattcatcg atccaggaac ttggcgccat
     4561 tttcggcatc gacttcgccc caaccggcga gaacaatgcg gacaccaaga tcaaactcgc
     4621 ggctcgcggc ggcgcttccg ccgtacctat gccagcagtg tcgacaccgg gcgtttcgtc
     4681 agccgaagcc tttgtggaat ctctattcga cccaatcagc ggcaagccga tcgatgtcga
     4741 tgctgacgcc gaaggagaga agaagagccg cggacagcga aagccggtca gacagaaagc
     4801 acggcagagc gtcatggtac cgacagcggt tacgaaaaag tccgcgcaaa caatcagcaa
     4861 gcgcaggaag ttgacaaccg ccgtgacggg gaaaaacaga tccgggagat cagcagacaa
     4921 gacgatccga cacaagaagc agtcagcaga cagcagtctg gtcaatcggt gtcagccgcg
     4981 acagatgctg aaattgtccg agttgcgcga gctcggcatt ccctattcca gagtgcaaaa
     5041 agccctactc gacggtcatc tcgagatcac cgcttggcgc ggcgagtatc gttgcacccg
     5101 cacgaccacc aactggctga agaattttgt ccgggtttca atttctgaaa tcgctgattg
     5161 atcgacgaca aggagtgtca ttttcatggc cgatctcgac attcgctgga aacaacgact
     5221 ctctcattac caacaggcac tgcttcagct cacccaggcg gtcgagctga gccgctcacg
     5281 agcattgtcc gatctcgaga agcaagggct gattcaggcg ttcgagttta cgcacgagct
     5341 tgcctggaac gtgatgaaag actatttcgt cgatcagggg aacccgaata tcaccggctc
     5401 tcgcgacgca tttcgcgaag cctttcaaaa gggattgatc caggaaggcg aaaactggat
     5461 gaacatgatc aagagccgca atctggcatc acacacgcac aatcgaaaaa cggccgaaga
     5521 gctggtacaa gcgatcttac atacctacca taaccttttt cgacagtttg ccgaaaccat
     5581 gcagaagctt gccgatgccc gacattcctg aaacgacccg cttcggcctg cgagaagaaa
     5641 caataaggct gattcaacag gtgtttgctt ctcacccgca cattgaacaa gcgattctct
     5701 acggatcgcg cgccaaaggc acacaccgag acgcctcgga tatcgatctc accctggtcg
     5761 gcgcaatgct cacgctgaac gattcctgtg cgattgccca ggaactcgac gacctgcttc
     5821 tcccctggaa gatcgatctt tcgcttcggc acacgatcga caatcccgct ttgcttgatc
     5881 acatcgaacg tgtcggccgc gagttctatc gtcgccccgc ataggcaatt ctgcgaaggt
     5941 tgccggcctc gacactcatt tcggcaccgt gaagcgctga acgatttttc acctcagcac
     6001 gcaggcgtca gttttccacc tttcaccgtg tatgctgagt tgcaaaccgc gcaggtatgc
     6061 gtggcacttt ccttcaggtc tttgccggaa accagcttca cgccgcagtg gcacatccac
     6121 ccggtgatgc ggcccgggtt gccgacgatc agcgcatacg ccggaacatc tttcgtgacc
     6181 accgatccgg cgccgatgaa gcagaactcg ccgagcgtgt gaccgcagac gacggtcgca
     6241 tttgcgccga tcgaagcccc gcgcttgatc agagtcttct tatactcatg ctttcgaatg
     6301 acgtgactgc gcggattgat gacattcgta aaaaccatcg acggcccgca gaacacgtcg
     6361 tcttcaagct caacgccgtc atagatcgac acgttgttct gcactttgac attgttgccg
     6421 atcacgacgt tcgggccaac gacgcagttc tgtccgaaac tgcacttctc gccgatgcgt
     6481 gtcttgccca gaatgtggca gaaatgccag atcttcgtgt ctttcccgat caccacgcca
     6541 tcatcaacgt atgaagattc atgcacaaaa aagtcgttcg ccatggttgc actcctgatg
     6601 ttcagtgtcc gtcgccggtc aagataccgg ctcatcgcag catctgtcca gcgaattcat
     6661 gtactcgtca aggctcacgc gcgatggtct ctgagccact gggcgatcaa ttctccggcg
     6721 ttgccgcgcc cgtagagttc gggacgactt ttggatagcc agttttcgtt ccaggcggct
     6781 tgcactgcgc gtatcacgtt cgtcggatca gtgccgacca cttggttcca accggcttcg
     6841 accagctcga cccactcggt ttcggtacgc aacgtcacac agggagtgtg atgaaagtag
     6901 gcttcctttt gtacccctcc ggaatcggtg acaatcagac gagcggcctt ttccagagcg
     6961 accatctcga gatacccgac cggatcgatc agcttcaggc gctggccgcc gcgttcgaga
     7021 agccgcgaca gatcgccgcg tttttccaga aggtgccggg tacgcgggtg aatcggccaa
     7081 acgaccggcg cgaattcgct tccgatcttc gtcagcagtt cgaccacccc ggcgagcgtc
     7141 gcggcatcat ccgtcgtttc ggcacggtga atggtcgcca gaatgaacgc tttcggcttg
     7201 agtgacaggc cggccaccga agtcaacacc ggcgctcgtg ttccatagaa caatgccgca
     7261 tcgtacatga cgtcgccgca atagacgact tgtactttag gcagacgtga cgacggcagt
     7321 ccttcgtgac ggaggttgtc gacggccgtc tgcgtggggc agaacagcgc ggtcgagacg
     7381 tggtcggtca tgatgcgatt gatctcttca ggcatgcggc ggttgaacga acgcagtccc
     7441 gcctcaacat gcgccaccgg tatatggagt ttcgccgcga ccaacgctcc ggccaacgtt
     7501 gagttggtgt ccccgtagac gagcacccag tcaggttttt catcgagcgc gactttttcg
     7561 atggcggcta gcatcgcgcc ggtctgcgct ccatgcgacc ccgagccgat gttgagcgaa
     7621 tgggtcggcc gcggaatatc catttcgtcg aagaacacct gcgacatgtt cgcatcgtaa
     7681 tgctgacccg tgtgaacgat cagctcgcga atcggctcgc cgccgcccgc cgcgcttcgt
     7741 aacgcccgac tgaccacagc ggccttgatg aactgcggcc gcgcccccag aaccgtcatc
     7801 agcttcatgc gtgtatcacc tcgttttcat ctcgccccac gatgtactcg attttgcctc
     7861 gtctgtcgac ccgcgaaatt gcctcgtggt aagattccgg tgaaccactc tgatcgcaac
     7921 ctgctcccgt cacaggaggc atcatgaccg gatctcaaca aaaccgttct tcgtcttcga
     7981 ctgcgctttc aacaagcgct tcccgttgcg gcaagaagcc ctgtacatac gcggacctgc
     8041 aaaagatgcc tgctgacgaa ctgtgggagc tcatcgacgg ggtgccgcac gccatgacgg
     8101 tgccttccgc cgatcatcag tttgtttcgg ttcaactcac ccgccagctc gcgaattttt
     8161 tccatcgttc tccctgtcag gtgtttcatg cgccctacgg cgtgtttctg ccgaagaaga
     8221 acgaatcgat cgaaaagacc acgaatctgg tgattcctga tctggtcgtc gtctgcgatg
     8281 agaagaagcg cacgccgcag ggctgtcgtg gagcgcccga cctggtcatt gagattctct
     8341 cgccatcgac ggccggacga gatcgcgtca agaaactcaa cctctacgaa cgtttcggcg
     8401 tcaaggaatt ctggatcatc agtctggaca gtgccgcggt catggtgttt cgcctcgatg
     8461 ccgacgggcg ctatgccagg ccggtgcagt tctactggtc tgacaagctc acgacaccac
     8521 tgttccccgc gctggaagtc gacctgagcg aggtatttcc gaagtttgtc gacaccgaag
     8581 atcccgaaac ccccgacgat tcaccgccac cctccacccc atccgacgcc cccgcgcccc
     8641 gcgtccgcga agacgccaaa ccgtttgccg cccccgccaa acgccgatca ggtcagagaa
     8701 aatcagccac caagcgtcgt taacagtttt ttgtcaacaa gtatattgga caatgtcctt
     8761 taaatgctgg ggagggccac atgcaagctg tgaactactc gactgcccgg aacaacttga
     8821 agtccatcat cgaagatatc gattccaacg gcaaagaata tcttgtcgtc acgaaagagg
     8881 atcggcaggt ggtcatcatc tcgtatgatg agtatcagtc aatgaaagaa acgctttacc
     8941 tgctttcgac gcccgccaat agcgaacgcc tgctttggtc gatcggccag atcgagaagt
     9001 cgaagtttca gcgatacaaa ctcatcgacc cgtgatcatc gcgttgtctg agatcggttg
     9061 gggggac
//
LOCUS       DAORHD010000022         8979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7207_length_8979_cov_3.854550, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000022 DAORHD010000000
VERSION     DAORHD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7207_length_8979_cov_3.854550"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PKO06_01045"
     CDS             complement(<1..536)
                     /locus_tag="PKO06_01045"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV68252.1"
                     /translation="MPDLKEFARKHLTWLGHDSFLIHAGKTIYIDPYQITDAKPADIV
                     LITHEHFDHCSPEDIAKIQHDKTVVVAPEDCLGKLTGNVRSIKPGMKIEIHGIEIEAV
                     PAYNVGKKFHPRLNAWVGYILTLEGIRLYHAGDTDFVPEMKTLRTDIAMLPVSGTYVM
                     TAEEAVQAALAMRPKLAIP"
     gene            651..6803
                     /locus_tag="PKO06_01050"
     CDS             651..6803
                     /locus_tag="PKO06_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HNV68253.1"
                     /translation="MNDDHLPVGDAANVSNDPVVARVPPEPGEQPESAAAPGASLVVM
                     ELDADGRISWAMECNGLPLIRRLGVENRSSAVLEHARLEITFADLATPVLLPIPRLEP
                     QQSWAVENPNVVLNGSRLRSLSERERVTLTVRCLHEDRELARTEQPIDVLAGNEWDRG
                     RLPELLAAFVTPNQPCIATLLQLARPTLERETGNAGFAGYSGRSRERVYAMTQAIYEG
                     LSAFDLTYVPAPVSFEANGQKIRLVDELMRHRLGNCLDVSLAVAAALEQAGLHPVLIL
                     FQKHAVPGVWLVNEWLPLTVSDDQLIVDKLVRCGDLLLFEATAALQKPAPNFETAVQQ
                     AEERLHSSDRFEMILDVRAARLARLLPLPLRREVDDPVLAESMIQQHIGTSATGTVRE
                     SNHESGLPSLEAAPMFPRIEGWKERLLDLSLRNRLLHYRPSREKTVPLAVCELPELED
                     RLADGRQLILEPLPELSTVEPRAAELVVDRGGEKQFEVERRRLLEQDRLLTSLDEAEL
                     QRRCRAIFRQARTELEETGSSTLFLALGFLRWFESEQATSPRLAPILLYPVEILRPAL
                     RGRYVIRVRDDEPRLNDTLIEKLRREFQLDFTPLKTLPLDESGIDLATVLSLLRQVTA
                     RIPRFDVVDQSVLGFFSFAKFLMWRDLTANLDDLMRNELVRYMVEGQANRAFADQAFA
                     EEGQLDTLYPPDQPPIVLDADSSQMTAIDAALRGNTFVLQGPPGTGKSQTIANMIAAV
                     MATGRRVLFVAEKRAALEVVARRLRTVGLGDACLELHSNRASKRDVALELFRVYQLGP
                     ETDHADLTQVVSELTSDRQTLNAHAALMNAETALGPSHHALFGRLIAGCATDEAMGKE
                     RRPLSQGTLIPRPLDVNAATWRQWQQLIREAADAVLPFFPPEAHALRGCHLLEWTPAL
                     EREREQEMQRCSAAAAELVRRLDAVQTALGCRFQARVKHLRVLDRLLGILEHQSREGV
                     AELLRRADVEKVLERLDQLLAWSVERETLWQSLETSFLPAMLDLDLVRLQREMLTYRD
                     SFGPWRWWKLRGCWAELAAVARGPVADLPRMLTQIGTGIRLQELSRQIRSEEELLVTL
                     LGPSLHQRLPNQREIATLVCSVREWRETGVSLPHDSLVFLPDPNGREPVERWRLSERS
                     ENSLLALPCARAAEYRRELGQIVSEFSECWARAMVGWRIRPVEVFQAEGDELIPAHLQ
                     TQAELWLKHVPALRDWSRWSRAVDAFEQAGLTGVVHRLALETVPVAMWEKRFELWLAQ
                     ERLNALCASDRNVAEFAGRRHDRTVERFRADDCELITLCRRQLRARLAARRPAFGAAV
                     SEFSEVGILQKEARRKRGHLPVRRLLERIPHLLGRLKPCLLMSPLSIAQYLPPGGEPF
                     DLVIFDEASQIAVHDAIGAIARGRQVIVVGDSKQLPPTSFFQADAEGGGEDAVDLDAE
                     QLLARDLESILEETAAALVPSLMLRWHYRSRDERLIAFSNHRYYDRRLQTFPAADRRA
                     ESGVSLIPVTGVFQRGGSRTNPAEAIAVVHAVVSHLRDENRRHLSLGVVTFNQPQQML
                     IEDLLDDARSQYPEIEPYFTERVPEPVFVKNLENVQGDERDVMLFSITYASDESGNVS
                     MNFGPLNRSGGERRLNVAITRARHRLLVFSGLSPESIDLHRTSAIGVRHLRDFLRYAA
                     GRDPEITVVSGDTGSATSLAPRENHLVAVIADFLRRHGYLVEERVGAARYRLDLAVRS
                     PDDGRVLMGIETDGDAYGSPGTARDRERLRAEVLTQLGWKLCRLYVLDWLNHPEIEQK
                     RLLALLQKARSETVPPEPPGANAQLVVEIAGPSNGGVPETAQGSSSPVTGVENGVENT
                     APSTHTSEEAQTPGLPPFVFLTTEKLGDADAFLRRDQNECLQQLLRRLTELEGPVHVD
                     RVAKVLTQAYGMTRVGSRAAQRVDALVQEICAAGDVCNHDEFLWPPGLDPSLNLPPAR
                     AAAADGTTRPPEQIAPEEIAAAALHLLERSVSLPRSELVRHTAAIMGFRRLTPRTQPF
                     FAAGIQVLLDRGLAEADGESVRLPRGAAPDQEHA"
     gene            6928..8262
                     /locus_tag="PKO06_01055"
     CDS             6928..8262
                     /locus_tag="PKO06_01055"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNV68254.1"
                     /translation="MSRFIRWAIVLWCCLPAVCSYGGGFMVREHSQAALGSAFAGVHA
                     GAHDLADMFFNPAALYYQPERAALAGMASMLPKAKFRLSSATTFNGTVVTGNPGGENI
                     GEDALIPSLYLAQPSWKGWRLGLSVNAPWGLATKCDPNWVGRYYAIESRLTSMNVMPT
                     VARRLSPRWVAGIGFQMQKLDAKLSNAVDYGTIAQANGVPGAAPTQQDGLVELTGEDW
                     GYGLVLGFMYEASARTRFGFSYRSKVDQNLKGTAEFSPDAFGVKNGLSALSGAFVTTG
                     AQAAVTTPEVVGFGVKHEMTASMTLVFDAVWNRWSRYKGLTVHFDNPAQAASRVQEDW
                     RDVWLTSIGLLYKVAPKWTLRAGYAVDRSPIPNEHRIPRIPGSDGRFTSVGARYQASE
                     RCHIDVGYMSISYDGAPITLKSTDPDSTFRGNLSGRFSNSFRILGAQIAWNW"
     gene            complement(8273..>8979)
                     /gene="phnD"
                     /locus_tag="PKO06_01060"
     CDS             complement(8273..>8979)
                     /gene="phnD"
                     /locus_tag="PKO06_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV68255.1"
                     /translation="TDEELAEHFSLLTAFLSRHLGIPVRVQVADDYRSLIQLIEAGKV
                     QVAWLGTALLETWDKKDMVEILCHPIQRGRAHYRSAILVRQDSPFRTLTDLAGHRMAY
                     VDRFSGSGFLYPARLFREQGLDPVASFSETLFSGNHSRSLEALLSGTTDAAAIFIDSG
                     DESGIPTGTRCLSWVQWIPTDPIVVRRDLATDTRQRLKQLFINIGQYPGGLETMQHLK
                     ARRGYEGFLADEELKN"
BASE COUNT         1674 a         2544 c         2808 g         1953 t
ORIGIN      
        1 ggaatcgcca gtttcgggcg catcgccagg gcggcttgaa ccgcctcttc cgcggtcatc
       61 acgtaggtgc ctgagactgg cagcatcgca atgtcggtgc gcagtgtttt catttcgggg
      121 acgaaatcgg tatcgcccgc gtgatagaga cggattccct cgagggtcag aatgtatccg
      181 acccaggcat tgagccgcgg gtggaacttc ttgccgacat tgtaggcagg caccgcttcg
      241 atctcgatgc cgtgaatctc gattttcatg cccggcttga tcgatcggac attgccggtt
      301 agcttgccga gacaatcttc gggagcgacg acgacggttt tgtcgtgctg aatcttcgcg
      361 atgtcttccg gagagcagtg gtcaaaatgc tcgtgggtga tcagcacgat atctgccggc
      421 ttcgcgtcgg tgatctgata agggtcaatg tagatcgtct tgccggcgtg gatcaggaag
      481 ctgtcgtggc caagccaggt gaggtgtttg cgggcaaatt ccttgaggtc gggcatgagt
      541 cctccgaggc ggtatcgaat acgtcggcgg tattgtaaca cccttttgcg tgtacgaaaa
      601 cgttggcgag ggtggcggac tggtgcgaat ctgagatatg ctgagacatc atgaacgatg
      661 atcatctgcc cgtgggcgat gcggcgaacg tgtcgaacga tcctgtggtt gcacgcgtgc
      721 caccagaacc aggcgaacag ccggaatccg ctgcggcgcc gggggcatcg ctcgtggtca
      781 tggagctgga tgccgatgga cggatcagct gggccatgga atgcaatgga ttgccgctga
      841 ttcgtcgact cggggtcgag aaccggtcga gcgcggttct tgaacatgct cgactggaga
      901 tcacttttgc cgatctcgca actccggttc ttctgccgat tccgcgtctc gaaccacaac
      961 agtcgtgggc ggtcgaaaac ccgaacgtgg tattgaacgg cagtcgcctg cggtcactca
     1021 gtgagcggga acgggtgacg ctgacggttc gttgcctgca cgaagaccgt gaattggcgc
     1081 gaacggaaca gcctatcgat gtcctggcgg gcaacgagtg ggaccgcggg cgtttgccgg
     1141 agctgctggc ggcgtttgtc acgccgaacc agccttgtat tgcgactctg ctgcagctgg
     1201 cgcgcccgac actcgagcgc gagacgggaa acgccgggtt tgccggatac tccggtcgtt
     1261 cgcgtgaacg ggtctatgcc atgacccagg cgatctatga aggtttgagc gcattcgatc
     1321 tgacgtatgt tcccgctccc gtgtcatttg aagcgaacgg gcagaagatt cgcctggtgg
     1381 atgaactgat gcggcatcgc ctgggaaact gcctcgatgt ctctcttgcg gtcgcggctg
     1441 cgctcgaaca ggctggcctt catccggtgc tgattctgtt ccagaaacat gcggtccccg
     1501 gggtgtggct ggtgaacgaa tggttgccgc tcacggtcag cgacgaccag ttgatcgtcg
     1561 acaagctggt cagatgcggt gatctgttgc tgttcgaggc gaccgcggcg ttgcagaagc
     1621 cggctccgaa ctttgagaca gcggttcagc aggcggagga acgtctgcac tcttcagatc
     1681 gcttcgagat gattctcgac gtgcgcgcag cgagactggc ccgactgctc cccctgcccc
     1741 tgcgccgtga agtcgacgat cccgtcttgg ctgaaagcat gatccagcag catattggaa
     1801 catcagcaac cggtacggtt cgcgaatcga accatgaatc tgggctccca tcgctcgagg
     1861 ctgcgccgat gtttccgcgc atcgagggct ggaaggagcg actgctcgac ctgtcgttgc
     1921 gcaatcgtct gctgcactat cgtccgagtc gcgaaaaaac cgtgccactg gccgtgtgtg
     1981 aactcccgga actcgaagat cgactggcgg acggtcggca gctgatcctc gaacctctgc
     2041 ccgaactttc gaccgtcgaa ccccgtgccg ccgagctcgt cgttgatcgt ggcggggaaa
     2101 aacaattcga ggtcgagcgt cgtcgtctgc tcgagcagga tcgtttgctg accagcctgg
     2161 acgaagcaga actgcagcgt cgctgtcgtg cgatttttcg ccaggctcgt accgaactcg
     2221 aggaaaccgg ttcgagcacc ctgtttctcg ctctcggctt tcttcgctgg tttgaatccg
     2281 agcaggcgac ctctccgcgg ttggctccga ttctattgta tccggttgag attcttcgac
     2341 cggcgctgcg cgggcgctat gtcatacggg tgcgcgatga tgaaccgcga ctgaatgata
     2401 cgctgatcga aaaactgcgt cgcgagttcc aactcgactt cactcccttg aagacgctgc
     2461 cgctcgatga gtcgggaatc gatctggcga ccgtgctgag cctgctgcgc caggtgaccg
     2521 cccgtattcc gcgatttgat gtggtcgatc agtcggtgct cggattcttt tcttttgcca
     2581 aatttctcat gtggcgcgat ctgacggcca acctcgatga cctcatgcgg aatgagctgg
     2641 ttagatacat ggtggaaggg caggccaatc gagcttttgc cgatcaggcc tttgccgagg
     2701 aaggccaact cgacacgctg tatccgcctg atcagccacc catcgttctc gatgccgaca
     2761 gcagtcaaat gacggccatc gatgccgctc tgcgtggaaa caccttcgtc ctgcaggggc
     2821 ctcccggtac cggcaaatca cagacgattg ccaacatgat tgccgcagtg atggcgactg
     2881 ggcggcgggt actctttgtc gccgagaaac gcgccgcgct cgaagtggtg gcccgcagac
     2941 tgcgtaccgt cgggctggga gatgcctgtc tcgaattgca tagtaaccgg gccagcaaac
     3001 gagatgttgc cctcgaactt tttcgagtgt accagctcgg ccctgaaacc gatcatgcgg
     3061 atctgactca ggtggtttcc gagttgacga gcgatcgtca gaccttgaat gcgcatgctg
     3121 ccttgatgaa tgccgagacg gctctcggtc cctcgcacca tgcgttgttc ggcaggttga
     3181 ttgctggttg tgcaactgac gaggcgatgg gaaaagagcg ccgtccgctg tctcagggaa
     3241 ccctcattcc gcgaccgctc gacgtgaatg ccgcgacctg gcggcaatgg caacaactga
     3301 tacgagaagc cgctgacgcg gttctgccat tctttccacc cgaagcgcat gccttgcgtg
     3361 ggtgccatct gctcgaatgg acacccgcgt tggaacgtga gcgcgagcag gaaatgcagc
     3421 ggtgttccgc cgccgccgcc gaactggtgc gcaggctaga tgcggtgcag acggcattgg
     3481 gatgccgctt tcaggcccgg gtcaaacacc tgcgggttct cgatcgtctg ctcggaattc
     3541 tggagcatca gagtcgtgag ggtgtcgccg aactgttgcg tcgcgcggat gtcgaaaaag
     3601 tgctcgaacg tctcgatcag ctgctggcct ggtcggtaga acgcgaaact ctctggcaga
     3661 gccttgagac gagcttcctg ccagcgatgc tcgatctcga cctggtgcgg ctgcagcgtg
     3721 aaatgctgac ctatcgcgat tcgttcggcc cctggcgctg gtggaagctg cgcggctgct
     3781 gggccgaact cgcggcggtc gcgcgcggtc cggttgccga cctgcctcgg atgctgacgc
     3841 agatcggcac gggaattcgg ctgcaggagc tgagtcggca gattcgcagc gaagaagaac
     3901 ttctggtcac tttgctgggg ccgtcgttgc atcaacgtct gcccaatcag cgcgagattg
     3961 ccaccctggt gtgttccgtg cgtgaatggc gcgaaacggg agtctcgttg ccgcacgatt
     4021 ccctggtgtt cctgcctgac ccaaacgggc gcgagccggt cgaacgctgg cgcttgtctg
     4081 aacgaagcga aaattccctt ctggcgcttc cttgtgccag agcggccgaa tatcgtcgtg
     4141 agctgggaca aatcgtcagc gagttcagtg aatgctgggc ccgggcgatg gtgggctggc
     4201 gcatcaggcc ggtcgaggtc tttcaggcgg agggggatga gctgattccg gcgcatctgc
     4261 agacccaggc cgagctctgg ttgaagcacg tgcccgctct tcgcgactgg agtcgttgga
     4321 gccgggccgt ggatgcattt gaacaggctg gtctgacggg tgtcgtccat cggctggcac
     4381 tcgaaacagt accggttgcg atgtgggaaa agcgttttga attgtggctc gcgcaggaac
     4441 ggttgaatgc tctctgtgcc agtgatcgga acgtggccga gttcgccggt cgacggcacg
     4501 atcgcaccgt tgaacgtttt cgcgccgatg attgcgaact gatcaccctc tgtcgtcgtc
     4561 agttacgggc tcgtctggcc gcgcgtcggc cggcattcgg tgcagccgtc agcgagtttt
     4621 ctgaagtcgg cattctgcag aaggaagccc gccgcaaacg cggacacctt cccgtgcgcc
     4681 gtctgcttga acgcattccg catctgcttg gtcggctgaa gccctgtctg ctgatgagcc
     4741 ccctgtcaat tgcccagtat ctgccgcccg gcggtgaacc gttcgatctg gtgattttcg
     4801 acgaagcctc acagatcgcg gtgcatgacg cgatcggtgc catcgcccgc ggtcgtcagg
     4861 tgatcgtcgt tggcgacagc aagcagttgc cgccgacttc tttcttccag gctgatgctg
     4921 aaggcggtgg cgaagatgcc gtcgatctcg atgccgaaca gttgctggca cgcgatcttg
     4981 aaagcattct ggaagaaacc gccgcggctc tggtgccgag tctgatgctg cggtggcact
     5041 atcgcagtcg cgatgagagg ctgatcgcct tcagcaatca ccggtactat gatcgacgac
     5101 tgcagacatt cccggccgcc gatcgacgag ccgaatctgg tgtctcgctg attcccgtca
     5161 ctggcgtgtt tcagcgtggt ggcagtcgga caaatccggc cgaggcgatc gccgtggttc
     5221 atgcggtggt ctcgcatctg cgtgacgaga accgacgtca tctgtcactg ggcgtggtca
     5281 cgttcaatca gccccagcag atgctgatcg aagatctgct cgatgacgcg cgatctcagt
     5341 atcccgaaat cgagccgtat ttcaccgagc gtgttcctga gccggtcttt gtgaaaaatc
     5401 tcgagaatgt acagggtgat gaacgtgatg tcatgctatt ttctatcacc tacgcctcgg
     5461 acgaatctgg aaacgtgtcg atgaatttcg ggccgctgaa tcgttcaggc ggtgaacgtc
     5521 gtctgaatgt tgccatcaca cgggctcgtc accgactgct ggttttttcg ggcctgagtc
     5581 ccgagtcgat cgatctccat cgcaccagtg cgatcggcgt tcgtcatctg cgtgactttc
     5641 tgcggtatgc cgcgggccgt gacccggaaa tcacggtcgt ttccggagat acgggctcag
     5701 cgacctctct ggcacctcgc gaaaaccatc tggtcgcagt gattgctgat ttcctgcgtc
     5761 gtcatggata cctggtggag gaacgggtcg gcgcggcgcg atatcgcctc gatctggccg
     5821 ttcgttcgcc tgatgacggg cgagttctta tgggaatcga gacggatggg gatgcctacg
     5881 gaagcccggg tactgcgcga gatcgcgaac ggctgcgtgc cgaagtgctg actcagcttg
     5941 gatggaagtt gtgtcgtctc tacgtgcttg attggctgaa tcatccggaa atcgaacaaa
     6001 aacggcttct ggcgctgctg caaaaagcac gttctgaaac tgtccccccg gaacccccgg
     6061 gagccaacgc ccaattggtc gtcgagatcg ccgggccgag caacggcggc gtacctgaga
     6121 cggctcaggg aagttcgagc ccggtgaccg gtgtcgagaa cggcgtggag aacaccgccc
     6181 catccacgca cacgagtgag gaggcacaaa ctcccggttt gccgccgttt gtgtttctga
     6241 ccaccgaaaa actcggcgat gccgacgcct ttcttcgccg tgatcagaac gaatgcctgc
     6301 agcagctgct gcgacgcttg acggaactcg agggaccggt gcatgtcgac cgtgtggcga
     6361 aagtattaac ccaggcctac ggcatgacca gagtcggttc tcgagcggct cagcgtgtcg
     6421 atgctctggt acaggagatc tgtgctgccg gagacgtgtg caatcacgat gagtttctct
     6481 ggcctcccgg actcgacccg tcgctgaacc tgccgccggc acgcgcggca gcggcagatg
     6541 gaacgacgcg tccgccggag cagattgcac cggaagagat tgctgctgca gctctgcatc
     6601 tgctggagcg gtccgtgagt ctgccgcgtt ccgaactggt gcgccatacg gcagcgatca
     6661 tgggctttcg ccgactgacc ccgagaacac agccattctt tgcggccggc attcaagtgt
     6721 tactcgatcg tggactggca gaggcggatg gcgaatcggt tcgactcccc cggggtgccg
     6781 ctcccgatca ggaacatgcg taaacgctgc ggtcgttgtc gccttggcgc gtacccgatg
     6841 tgagacaatt ttccttgatt tgtcttgctt gatttcctat gatcagagaa cgcctttgtg
     6901 cgtatcactt ttcgagtggg agaatgtatg tcgcgtttta ttcgctgggc gatcgttctg
     6961 tggtgttgcc tcccggccgt ttgttcctat ggcggtggat tcatggtgcg agagcattcg
     7021 caggccgcgc tgggcagtgc ctttgccgga gttcatgccg gagcacacga tctggcggac
     7081 atgttcttca atccggcagc gctctactat cagcctgaac gagccgccct ggcgggcatg
     7141 gcgagcatgc tgccgaaagc gaagtttcgc ctctcgtcgg ccaccacgtt caacggcacc
     7201 gtcgtgacgg gaaaccccgg tggcgaaaac atcggcgagg atgcgctcat tccctcattg
     7261 tatctggcac agccttcctg gaaaggctgg cgtctcggcc tctcggtcaa tgcaccctgg
     7321 ggattggcca ccaagtgtga tccgaactgg gttggtcgct actatgccat cgaatcgcgg
     7381 ctcacctcga tgaatgtcat gccgaccgtc gcgcgtcgtt tgagcccgag gtgggtggcg
     7441 gggatcgggt ttcagatgca gaagctcgat gcgaagctct caaatgccgt ggactacgga
     7501 accatcgctc aggccaacgg agtgccggga gcagcaccga cacagcagga cgggctggtc
     7561 gagttgaccg gcgaggactg ggggtatggg ttggttctcg ggttcatgta tgaagcgtcg
     7621 gcccgaactc gcttcggttt ctcgtatcgc tcgaaagtgg accagaacct gaagggaacg
     7681 gcggagttct cgccggatgc cttcggcgtc aagaacgggt tatcagccct ctcgggtgca
     7741 tttgtgacga caggtgcgca ggccgcggtg accacgcctg aagtcgttgg tttcggcgtg
     7801 aagcacgaga tgacggcctc tatgaccctg gtttttgacg cggtctggaa tcgctggagc
     7861 cggtacaaag gcctgacggt gcactttgac aaccccgcgc aggcagcgag tcgggtgcag
     7921 gaagattggc gcgatgtctg gctgacatcg attggtctct tgtacaaagt tgctccgaaa
     7981 tggacccttc gcgccggcta tgccgtcgat cggtctccga ttcccaatga acaccgaatt
     8041 ccacgcattc ccggctcgga cggtcggttc acttccgtcg gcgcgcgcta tcaggcgtca
     8101 gagcgttgcc acattgacgt tggctacatg tccatcagct acgacggagc accgatcaca
     8161 ctcaagagta ccgatcccga cagcacgttc cgcggaaatc tgagcgggcg tttctcgaac
     8221 agctttcgga ttctgggcgc acagatcgcc tggaactggt agtcgcggtc ggtcagttct
     8281 tcagctcttc atcagcgaga aagccctcat acccccggcg tgctttcaga tgttgcatgg
     8341 tttcgagccc ccccgggtac tggccgatat tgatgaaaag ctgcttcaac cgctgacggg
     8401 tgtcggtggc aagatcgcgg cgcacgacga tcggatcagt ggggatccat tgaacccagc
     8461 tgagacagcg tgtccccgtc ggaatgccgg attcgtcacc ggagtcgatg aagatcgctg
     8521 ccgcatccgt ggtcccactc agtaaagctt ccaggctgcg actgtggttc ccactgaaca
     8581 gcgtctcgga aaacgaggcg actgggtcca ggccctgttc tcggaacaac cgcgccggat
     8641 acaggaatcc actgccggaa aagcggtcga catacgccat gcgatgtccg gccagatcgg
     8701 tcaacgtgcg aaaggggctg tcctgccgca cgagaatcgc tgaccgatag tgagcccgac
     8761 cacgttgaat cggatggcag agaatttcga ccatgtcttt tttgtcccac gtctcgagaa
     8821 gcgctgttcc gagccaggct acttgtactt ttccggcttc gatcaattga atgagcgaac
     8881 gatagtcatc ggcgacctgt actcgtaccg gtatccccag atgtcggctg agaaaggccg
     8941 tcagcagtga aaagtgttcc gctaactctt cgtctgtct
//
LOCUS       DAORHD010000023         8842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7362_length_8842_cov_3.208831, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000023 DAORHD010000000
VERSION     DAORHD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7362_length_8842_cov_3.208831"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..1790
                     /locus_tag="PKO06_01065"
     CDS             198..1790
                     /locus_tag="PKO06_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HNV68256.1"
                     /translation="MRNPKTDSEKDLNSERLNEIRRKVAATAAAEAKKTSTKSRRLSS
                     AALAKALKDGEAGDRNIIVRLGKGRFLFDYSTEQFFLFRDHRWCVDEKRQHLVLVDEY
                     KALLLSESRRIKRAMKQPGYNSAARAGDAKRVKALKERVDALNRLEYRRRILDFSTVG
                     EAGLGITGKEWDMNTHLLCFMNGVLDLRTLEFRDGRPEDYLQMQIPCQWTGIDTPAPA
                     WEKALEAILPLDPLAPDRGGDIEVIKYLQKAVGYSLLGDPREHILIILIGVGQNGKGT
                     ILEILGYVFGPLAGPVPSEMLLEQNFARSGSAPSADIMALRGRRLAWASESNEGRKLD
                     SGKVKWLSGGDTITARPPYGKKQITFRATHQLFLLTNHAPEVDPDDRALWYRLRLIPF
                     MMSFVETPTAPNERKRDKGLVARLQGEASGIAAWAIRGYREYLQTGLVAPSRVTMATS
                     EYRGQNDLISQFVSECLVADKAAAVTGSRLYATYQQWAEKSGQRPLPNNKFAARIREK
                     IGAPTKTSSGSEYRGYRARPSK"
     gene            2200..4755
                     /locus_tag="PKO06_01070"
     CDS             2200..4755
                     /locus_tag="PKO06_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HNV68257.1"
                     /translation="MIDKWSHALLACESTQEWLINERFVQPSTMRMFDLGWNDSDLFM
                     DGEEWGWVADDGKQQTVFVPRGLVIPIRRQQALSAIKIRAMGSKCSTRFHMVRGSLAT
                     PLVAHSRAEVVFIVESELDAILLSQEAGDLASFVAMGSAANKPDDDLDRLIRAATLVF
                     VALDNDEAGMNASKSNWVTRYPNTRRWAIPDEYGKDPTEARARGLDLRAWVLAALPDL
                     LNPLLATQSVQVETETIVLCEEAGPTSSPATVVAEPEETPLPLPVNYVYVTSADDIAS
                     ALGLLEGMQSVGLDIETFSTCPPRDKKDIPALDPYLNSIRLISVGNEQVQFLFDAQAL
                     GAEIEPVLDLIRRKTVIGHNLAFDLKTLAVKYGDHMLDVECYDTYLAVLILWYKSHHI
                     HPPKGAMRLGEVVERYLSRELDKTLQTSDFALPLSAEQLSYAAMDIQVLPALRDMLQS
                     ELRENEIDHRAVDLEMKFLPEKIRAEMNGLPVNEVGLHQRLSDISDQLGGTERFFDEL
                     DVNPNSPKILSVLRKQVPDLPSSDKESIRPYREQPVFRELCSYREFVHNYRFLKKALP
                     NIRDGRVSPAFIQISGPTGRMGCPGVMLQAIPRSLESLFYHPPEGWAILTADFPAIEM
                     RLAATIADDRALIDCFRNDEDPHRKMASRITDIPEDQIGKKSVHRQKAKAANFGFLYG
                     MGVDAFRKKCLADGVEFSEEEARRFRDTFFQMYDGIDLWHREAKNGLATPLCEVYVKK
                     LKRRVPAIEVKTIMGRSVLAPGYSAVLNYCVQGSGAELLKLTVVEVGRSMRQQHLAAQ
                     IVNMVHDSVSVLCPESEVGRVTEILNVCVRESAKVFMPDFDTVPEITVVRRNG"
     gene            complement(4837..5709)
                     /locus_tag="PKO06_01075"
     CDS             complement(4837..5709)
                     /locus_tag="PKO06_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68258.1"
                     /translation="MKITNRLNLPSALVNAVRNDPYSAGACDISVTRLVSPPRIVALE
                     RQFHDKIEEDVSDRIWTIFGQAVHHILERGEETAVTEERLSIERQAWVISGQFDRLVL
                     AEGLLQDWKVSTVYSAGNGGRFEWEAQLNLLGTILREHGVRVEKLEVVVLMRDWSKTM
                     SLRNPEYPEAAVIKIPVPLWSIERCEEYLDERIRLHQAARTELPLCEAQERWAKPDTW
                     ALKKVNRVKAVRVFDNEADAYAACEVAGNEHSVEHRPGQNMRCQNFCSCAAFCSQWQA
                     ILKSGGNAGSDDPA"
     gene            complement(5706..6512)
                     /locus_tag="PKO06_01080"
     CDS             complement(5706..6512)
                     /locus_tag="PKO06_01080"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HNV68259.1"
                     /translation="METSQTISKIAEALAKVQGQMKPAGFDASNPHFKSKYATLAAVM
                     ESCRQLLSAHGIAILQGTSVENDPLRVSVQTQLVHVSGEWIKETLSIRPVRDDAQSIG
                     SAITYARRYGLSAMVGIVSDDDDDGEAAVGRQNNQPEPVPGNVRKLTAVKKTVKSETA
                     KVDPPKPEPVMPATEKAYINKVPAKPNLDNPKTSVQDRIAKIREIFTFSAKLGQKPDQ
                     MKAAIGQILGLNRPIRESAEITNDRIDDVLEAFRADYLERFGNEGKEKAA"
     gene            complement(6593..7651)
                     /locus_tag="PKO06_01085"
     CDS             complement(6593..7651)
                     /locus_tag="PKO06_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNV68260.1"
                     /translation="MNTHEAPFHPTPVESHPHSTALTASVKHPVPLPDLIDYLHEVES
                     YENPDTVLDLKDLSFNTRQNLVFPSGAEHALNDWSQRQLGSLLGIRYDKWFEMATPEE
                     RAMQMNQRLCRFNGQMKFRTSWARDNSVEADGIVQAIVSPTFSVISDAFIADLLMTEN
                     LSLGLSVVRFHRTERTVSYMLGVGMPSYTDREVGNVWGCLSVQNSGVGFCSLTVTLSL
                     VRLVCTNGMTAPVANSVLLRRKHLGIAADDVAAKLTDSIRHAPELLAQGISNLVQSRQ
                     RPISNPEEALALLLSRAKIGNKHLPAFLAAYAREPQPSVFGIGQAATDAQTHLQIGLS
                     PEDRLQLENVTGEYLRTM"
     gene            complement(7713..7844)
                     /locus_tag="PKO06_01090"
     CDS             complement(7713..7844)
                     /locus_tag="PKO06_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68261.1"
                     /translation="MKKSPHPFGSLISIVIDVAIDRILKYLTETAEPTTKTDTEKGK"
     gene            complement(8028..8243)
                     /locus_tag="PKO06_01095"
     CDS             complement(8028..8243)
                     /locus_tag="PKO06_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNV68262.1"
                     /translation="MASSPNFSSALGFLRLPPVLQLIPVSKATWWAGVQSGRFPRGIK
                     LTPRTTGWRRSDILQLIAELEGEATKQ"
     gene            complement(8285..8482)
                     /locus_tag="PKO06_01100"
     CDS             complement(8285..8482)
                     /locus_tag="PKO06_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013241124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV68263.1"
                     /translation="MSEFFATFLTPAEVAKVLRSSVFHVRNLIKYGLLPAVRAGKRQL
                     RVSVKALGDYLEAQRVESNRR"
BASE COUNT         1990 a         2295 c         2590 g         1967 t
ORIGIN      
        1 aaaggtgatt ttttacaaga agtcgctcta caagtttacg ctcgattcgc cggttggggt
       61 gtaaaaaaaa tacgccccgg caggccgagg cgctctagca aacgcttata gaatgattat
      121 acggcggtaa ctgcctgtag tcaagcaaga gcgcatctcg accctccgaa atctttactg
      181 ataggagggt tagcgaaatg cgtaacccga aaaccgattc tgagaaagat ctgaacagtg
      241 agcgactgaa tgaaatccgc agaaaagtgg cggcaaccgc cgccgccgaa gcgaagaaga
      301 cgagtaccaa gtcgcgacgg ctgtcttctg ccgctctggc caaggccctg aaggacggcg
      361 aggcgggcga cagaaacatt atcgtccggc tcggcaaagg gcggttcttg ttcgactaca
      421 gcacggagca gttcttcctg ttccgtgatc accgatggtg cgtagatgag aagcgtcagc
      481 acctggtact cgtggacgag tacaaagccc ttctcctgag cgagtctcgc cgcatcaaac
      541 gcgcaatgaa acagccgggt tacaactcgg cggccagggc tggcgacgcc aaacgcgtga
      601 aggccctgaa ggagagagta gacgctctga accgcctgga ataccgacgc cggattctcg
      661 atttctctac cgtcggggaa gccggtcttg gtatcaccgg gaaggagtgg gacatgaaca
      721 cccacctgct ctgtttcatg aacggggtgc ttgacctgcg tacgctggag ttccgcgatg
      781 gtcgccccga agactacctg cagatgcaga ttccatgcca atggaccggg atcgacaccc
      841 cggcaccggc ctgggagaaa gcgttggaag cgattttgcc cctggatccg cttgcaccgg
      901 acagaggggg tgacatcgag gtcatcaagt atctgcagaa agctgttgga tactcgcttc
      961 tcggcgatcc ccgggagcac attctgatca ttctgattgg cgtcgggcag aacggtaaag
     1021 gcacgattct tgaaatactg ggctatgtgt tcggaccact tgccggtccc gtcccgtcag
     1081 agatgcttct ggagcagaac ttcgcgaggt caggaagcgc tccttctgcc gacatcatgg
     1141 ctttacgcgg cagacgcctc gcatgggcgt ccgagagcaa tgaagggagg aagctcgatt
     1201 ccggcaaggt gaagtggtta tcgggcggcg acaccattac ggcaaggcct ccgtatggca
     1261 agaaacagat cacgtttcgc gctacgcatc agctgtttct cctgaccaac cacgcgcccg
     1321 aggtcgaccc tgatgaccgg gctctctggt accggctcag attgataccg ttcatgatgt
     1381 cgttcgttga gactccgaca gccccgaatg aacgaaaacg agataaaggg ctagtcgcca
     1441 ggcttcaagg tgaagcatca ggcattgcgg cctgggcgat acgagggtat cgcgagtatc
     1501 tgcagactgg tctggtggcc cccagtcgcg tgacaatggc tacgagtgag tatcgcggcc
     1561 agaacgatct gatcagtcag ttcgtcagcg aatgcctggt ggcggacaag gcagccgccg
     1621 tcaccggtag tcgactgtat gcgacgtatc agcagtgggc agagaaaagc ggtcagcgtc
     1681 cacttccaaa caacaaattc gcggctcgca tacgagagaa gatcggcgca cccacgaaaa
     1741 cgtcatcggg ctcagagtat cgaggataca gggctcgccc atctaagtaa cacgtacaaa
     1801 tggaagctcc ggtggttcag tctgccggag catgactttc agtgaaggag tttttcgatg
     1861 aatctgctcg actgtctaat tgaggacgga tttcacccgg cgagaaaaac cgccggacat
     1921 agccctgaat actcctcgcc ctgcccattc tgtggaggcg aagatcgatt caacatctgg
     1981 ccacacgagg gcaatacagg tcgctggtgg tgcagaaagt gtcagcggtc aggtgacctc
     2041 gcatcatatc ttcatgagtt tcggcagtgg tctctcgcgg acgctataga gactgcaaat
     2101 acatctctcg atgcgccttt ctcaagttca ccaccagtac agtcgccacc ggaaaggaga
     2161 ggactatctg agacggtatg gtctatcgcg ggagagaaga tgatcgacaa gtggagccat
     2221 gcactcttgg cttgcgagtc tactcaagag tggttgatca atgaacgatt tgtacaaccc
     2281 agcacgatga ggatgttcga ccttggctgg aatgactcag acttattcat ggacggcgag
     2341 gagtgggggt gggtagccga tgatgggaaa cagcagacgg tgttcgttcc ccgtggcttg
     2401 gtcataccga ttcgcaggca gcaggcgtta tcggccatca agattcgagc catggggtcg
     2461 aaatgtagca cgcgcttcca catggtccgt gggtccctcg caacaccgtt ggtcgcccat
     2521 tcacgtgcag aagtggtttt catcgttgag agcgagctcg atgcgatcct gctgtcgcaa
     2581 gaagcaggcg atctcgcgtc attcgtggcg atggggtcag ccgcgaacaa gcccgatgac
     2641 gatcttgacc gtctgatccg tgctgcgacg ctcgtgtttg tcgccctgga caacgacgag
     2701 gcaggtatga atgcgagtaa gagcaactgg gtgacgagat acccgaatac gcgccgctgg
     2761 gccatacccg acgaatacgg aaaagatccg acggaggcca gagcgagagg gctcgatctt
     2821 cgcgcctggg tcttggcggc cctgccagac ctgctgaacc cgctgttggc gacacagagc
     2881 gtgcaagttg aaacagagac aatcgtgctc tgtgaagagg ctggtcctac ttcgagccct
     2941 gcgactgtcg ttgccgagcc cgaagaaaca ccgctcccgt tgcctgtgaa ctacgtgtac
     3001 gttaccagtg cagacgacat agcgtctgca ctgggtctgt tggagggcat gcagtcagtg
     3061 gggctcgaca tcgaaacgtt cagcacctgt ccgccgcgtg acaagaagga tattccagct
     3121 ttagaccctt atctcaattc tattcgactc atttccgtcg gcaacgaaca ggtacagttt
     3181 ctgtttgacg cccaggcgct cggggcagag atcgaaccgg tgcttgacct tatacgccgg
     3241 aagacggtta tcggccacaa tctcgcattt gacctcaaga ccctggcggt gaaatacggc
     3301 gatcacatgc tagacgttga gtgctatgac acgtatctgg cggtcctgat actctggtac
     3361 aaaagtcatc acatacatcc gccgaagggc gcaatgaggc tcggtgaggt tgtcgaaaga
     3421 tatctgagcc gtgaactgga taagactctg cagacgtctg acttcgccct tcccttgtct
     3481 gccgagcagt tgtcctacgc cgccatggac attcaggtcc taccagcact gcgagacatg
     3541 ctgcagagcg agctccgtga gaacgagatc gatcaccgcg ccgtcgatct cgaaatgaaa
     3601 tttctgccgg agaagatccg ggccgaaatg aacgggttgc cggtcaacga agtcggtctt
     3661 catcagcgtc tatccgatat cagtgaccag ctcggtggga cagaaagatt ctttgatgag
     3721 ttggacgtga acccgaatag tccgaagatc ctgtctgtgc ttcgtaaaca ggtgcctgac
     3781 ctcccatcat ccgacaagga aagtattcgt ccttacaggg agcagccggt gtttcgcgag
     3841 ttgtgttcat accgtgagtt cgttcacaat tatcgtttcc tgaagaaggc actccccaac
     3901 atccgcgatg gccgggtgtc tcccgcgttc atacaaatca gcggcccgac gggcagaatg
     3961 ggctgccccg gtgtcatgtt gcaggcgatt ccgcgcagtc tggagtcact cttctaccat
     4021 ccgccagagg gctgggcgat tctgacagcg gactttccag ccattgagat gcgcctggct
     4081 gctactatag ccgacgacag agcgctcatc gattgctttc gcaacgacga ggacccacac
     4141 cgaaaaatgg cttctcggat caccgacatt cctgaagatc agatcgggaa gaagagcgtg
     4201 catcgacaga aggcaaaagc cgcgaacttc gggtttctgt atggaatggg cgtcgacgcc
     4261 ttcagaaaga agtgtctcgc tgacggtgtc gagttctccg aggaagaggc ccggcggttc
     4321 cgggacacgt tttttcagat gtacgatggg attgatctgt ggcatcggga ggcaaagaac
     4381 gggctggcaa cgcctctttg cgaggtttat gtgaagaagc tcaaacggag ggtgccagcc
     4441 attgaggtga aaacgatcat gggaagatcg gttctggcac ctggttacag cgcggtgctg
     4501 aattactgtg tgcaggggag tggtgccgaa ttgctgaagc tgacggttgt cgaggtaggc
     4561 agatcgatgc gccagcagca ccttgcggcc cagattgtga acatggtgca tgacagcgtc
     4621 agcgttcttt gcccggagtc tgaggtcggg cgtgtcacgg agatcttgaa cgtctgcgtg
     4681 cgtgaatcag cgaaagtatt catgccggac ttcgacaccg tgcctgaaat caccgtcgtt
     4741 cgtcgtaacg gctgagggcg gtatcagtgc cagctaaaaa agaggagagc gcacatcaaa
     4801 gcgctctccc cgcaacgggg aatagagagg aacagatcat gcaggatcat cagatccggc
     4861 gttgccaccg gacttcagaa tcgcctgcca ctgtgaacag aaagcggcac acgaacagaa
     4921 gttctgacat cgcatgttct ggccggggcg gtgctcgaca gagtgttcat tgccagccac
     4981 ttcgcaggca gcgtaggcat cggcttcatt gtcgaagaca cgaacagcct tcacccggtt
     5041 gacctttttc aaagcccagg tgtcaggctt cgcccaacgt tcctgggctt cacacaaggg
     5101 cagttccgtt ctcgccgctt gatgcaggcg gatacgctca tccagatact cttcacatct
     5161 ttcgatgctc cagagcggga cggggatctt gatgacagcg gcctccgggt attccgggtt
     5221 tctgagagac atggtcttcg accagtcgcg catgagaaca acgacttcga gtttctcaac
     5281 tcggacaccg tgttcacgga ggattgttcc aaggagattc agctgagctt cccactcgaa
     5341 tcttccgccg ttgccagcgg agtagaccgt gctgactttc cagtcttgaa gcagaccttc
     5401 agccagaacc agtctgtcga actgacctga gattacccag gcctggcgtt cgatgctgag
     5461 ccgttcttcg gtcacagcag tctcttctcc tctctccagg atgtgatgga cggcctggcc
     5521 gaaaatggtc cagatacgat cactgacatc ttcttcgatc ttgtcgtgga actgacgttc
     5581 cagagcgaca atacggggcg gcgataccag gcgggtaacg ctgatatcgc atgcgcctgc
     5641 ggaatacggg tcattgcgaa cagcgttcac aagcgccgag ggcaagttga gccggttggt
     5701 gatcttcatg cggctttctc ctttccttcg ttgccgaagc gttcaaggta gtcagcacgg
     5761 aacgcttcga ggacatcatc gatgcgatca ttcgtgattt ccgctgactc acggatggga
     5821 cgattgagac ccagaatctg gccaatggcg gccttcatct gatcaggttt ctggcccagc
     5881 tttgcgctga acgtgaagat ctcgcggatc ttcgcgatac gatcctgaac gctggtcttc
     5941 gggttgtcca ggttcggttt cgcgggaacc ttattgatat atgccttctc agtggcaggc
     6001 attacaggtt ccggcttcgg cgggtccact tttgcggttt cagacttcac ggtcttcttg
     6061 accgcagtca gtttccggac attgccgggt acgggttctg gctgattgtt ctgacgacca
     6121 acagcggctt cgccgtcgtc gtcatcatca ctgacaatgc caaccatggc agagaggcca
     6181 tagcgacggg catacgtgat tgcgcttccg atagactgag cgtcgtctcg gacaggacgg
     6241 atggagaggg tttccttgat ccactctccg gacacgtgaa cgagttgcgt ctgaacgctg
     6301 accctcagcg ggtcgttctc gacggatgtt ccttgcagaa tagcgatacc atgtgcgctg
     6361 agtaactgac ggcacgattc catgacggcg gccagcgtgg cgtatttcga tttgaagtgc
     6421 gggttcgagg catcgaatcc agcgggcttc atttgcccct gcactttggc gagggcttca
     6481 gcaatcttcg atatagtttg agaagtttcc atacaggttc tcctttgatg tttctaaaag
     6541 actgaagggg tgcctttcga caccccttcg cgataggaca cgtgaggacg ggttacatag
     6601 ttcgcaggta ttcaccggta acattctcca gttgaaggcg gtcttcagga ctgagaccga
     6661 tctgaaggtg agtctgagca tcggttgcgg cttggccgat tccgaagaca gacggttgtg
     6721 gctcacgggc ataggctgca agaaatgcgg gcaggtgttt gttgccgatc ttggcacggg
     6781 aaagcaacag agcgagcgct tcctcgggat tcgaaatagg acgctgacgg gactgcacca
     6841 ggttgcttat tccctgggcg agtagttcag gagcgtgacg aattgagtca gtcagtttcg
     6901 ccgcaacatc atcggcggca atgccgagat gctttcgacg gagcaggaca gagttcgcga
     6961 caggcgcggt catgccattg gtgcagacca ggcgcaccag agacagggtc acggtgaggc
     7021 tacagaagcc aacaccggaa ttttgaacgg acaaacagcc ccagacatta ccaacctcac
     7081 ggtctgtgta actgggcatt ccaacaccga gcatgtagga cacggttcgt tcagttcgat
     7141 ggaaccggac aacagacaag ccgaggctca ggttctcagt catgagcaga tcggcaatga
     7201 aagcgtcaga tatgacgctg aaggtaggac tgacgatagc ttgtacaatt ccgtcggctt
     7261 cgacggagtt gtcacgagcc caggacgttc tgaacttcat ctggccattg aagcggcaca
     7321 gacgttgatt catttgcatg gcacgctctt caggcgttgc catctcaaac cacttgtcat
     7381 atcggattcc gagcagcgat ccaagttgtc gctggctcca gtcgttaagg gcgtgttctg
     7441 cgccacttgg gaagaccagg ttttggcgcg tgttgaagga cagatctttg aggtcgagaa
     7501 cggtgtcagg gttctcgtac gattcgactt catgcagata gtcgatcaga tcagggagtg
     7561 gcaccggatg tttcaccgat gcggtcaggg ctgtggaatg cgggtgcgat tccacaggag
     7621 tggggtggaa gggcgcttca tgcgtgttca tgcagagcct ccttgttgaa atagttaaag
     7681 gcagggccat aggcccgaga cggttgaaac cttcatttcc ccttttcggt gtcggttttc
     7741 gtggtgggtt cggctgtttc ggtcagatac ttcaggatcc ggtcaatggc gacgtcgatt
     7801 acaatcgaga tcagagagcc aaacggatgc gggctttttt tcatgacggt ctcacctccc
     7861 tttcagatgg acttcgagcc ggggaaaatc cagcttcaaa agccttatac caacgaagga
     7921 ggggaaaatt tagtcggatg agccgacggc aggctgcgga cagaaagtcc ggaaatgaga
     7981 aacgcgggca gaaacctgcc cgcgctgttt cgactgcctg cgacttatta ttgcttcgtg
     8041 gcttcccctt cgagttccgc gatcaattgc agaatgtcgg agcgtctcca tcctgttgtg
     8101 cgaggcgtca gcttgatccc acgtggaaaa cgtccggact gcaccccggc ccaccaggtg
     8161 gcctttgaga ccgggatgag ctgaaggacc ggaggtaagc gtagaaagcc cagtgctgac
     8221 gaaaaattcg gtgatgaagc cagtttctgt tcagtttcag atgaatcggg aagtggtggt
     8281 ttactcatcg tctgttgctc tccacacgct gcgcttcaag gtagtcaccc agggctttca
     8341 ccgaaactct cagttggcgc ttgcctgctc ggactgctgg taatagacca tatttgatga
     8401 gattgcggac atgaaaaacg ctggagcgga ggactttggc aacctcggct ggtgtaagga
     8461 aagtggcgaa aaattcagac atgaacttct cctttgtcgc gacttccggc ggaacaagcc
     8521 gccggttgcc tcaaggagag ctgtctgtta tcctgagaat gcgcgagctg gggtaacacc
     8581 cagtcctgct tgagggctac agaaaggaag ttggcgcttt ctttccgtag tcctcattct
     8641 acttcctgca ccgttcgttg tcaatgggtg cagattctgt tttttcgtgg tagtattttt
     8701 tctgcagaga tgcagccttg cagggtttac tgcagtattc ttggtcgcct ctgtttttct
     8761 tgaagacttt ggcgcaacgt gggcatagcc cgacgaaaaa ggaaacgttg ggcataggca
     8821 tctctgcccc gctgtcggga ca
//
LOCUS       DAORHD010000024         8810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7409_length_8810_cov_3.874015, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000024 DAORHD010000000
VERSION     DAORHD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7409_length_8810_cov_3.874015"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2306
                     /locus_tag="PKO06_01105"
     CDS             <1..2306
                     /locus_tag="PKO06_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV68264.1"
                     /translation="NGPVQQFGNPEGVRYRLFRWLKDGRRFVVCSDEPGEECLEVHTQ
                     DGSAPVKRLRAMPIGRPIELKVDPQSDRVALTNHRNELWLIDLEKQAHTHIATSRHAP
                     LRGFDWSPDGKWLAYNESLSTHTSAIFLYDVVKKKKTQVTLPVLNDVQPTFDPDGKYL
                     YFLSFREFDPVYDQLHFDLGFPRGIKPYLIVLSNENVSPFLPAPKPPAEPPKPAASDN
                     KTAPKSKPPAPTPPVTRIDLDGITDRVLAFPVNDGIYTRLAAISGKVYFGTQPVQGTI
                     SQNWHDKPANGTIECYDLELQKLDPVVSGVSDFRVSPDRKWLLYRTGNRLRLVKAGEK
                     PDEAAAREGPGRKSGWIDLGRIQISVNPQAEWRQMYREAWRLQRDHFWTEDLSQVDWE
                     KIYRRYLPLLDRLGTRGEFSDLVWEMQGELGTSHAYEMGGDYRLAPNYGLGRLAIDTN
                     WDEKLKGWKILALSRGDPWDERRSSPLLRPGINARPGDAIIAIGGRALSAHLTPDEAL
                     VNLANREVQVTILSKSQKKPRTVTVKTLADESASRYRDWVEQNRAFVHEKSQGRIGYV
                     HIPDMGPRGYAEFHRSFIAEVDRDGLIVDVRFNGGGHVSQLLLEKLARRRLAYVQTRW
                     FGTEPYPAESVAGPVIALTNENAGSDGDIFSHCFKLLKLGPLVGKRTWGGVIGIWPRN
                     QLVDGGLTSQPEFSFWFTDVGWQVENYGTDPDIEVDFLPQDHAAGRDPQLERTVGEIL
                     RLLKQKPPRYPDLGKGRPKKPLPKLPR"
     gene            2424..2915
                     /locus_tag="PKO06_01110"
     CDS             2424..2915
                     /locus_tag="PKO06_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV68265.1"
                     /translation="MRKLAFLLVAGLLIALPAFAVFCTTCGEKLPDSARFCSKCGGKI
                     TAPTPEKKTTPAVKEKPAAVEPAPTTEPAPAASAPSLAGTFRLKTDLYVYAKRGDEHN
                     VLKKNLFFKPRRDRIKANAEIKILEVVGDAYLVEQVAPVNGKGLKGWCTESELALRST
                     WTK"
     gene            2937..3569
                     /locus_tag="PKO06_01115"
     CDS             2937..3569
                     /locus_tag="PKO06_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNV68266.1"
                     /translation="MTNTQSSAKPARTTLAGFFEHREEIKHSRFIARACSVNTPDEAS
                     AWISQHRDPEATHNAWAWSMHGSFRFHDDGEVSGTAGKPIWGAIEKQGLDRVVVLVTR
                     YYGGIQLGAGGLARAYGGAAATCLRLAPKRLLQRVLSASFQIPFSEQGRTYHLFERYQ
                     IERLSETFTDRGVTFEVRLSPERLDGFRAALRDTLRSPDDHWILISDDLF"
     gene            3752..6205
                     /locus_tag="PKO06_01120"
     CDS             3752..6205
                     /locus_tag="PKO06_01120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNV68267.1"
                     /translation="MEFLCQSGDLVATFVGAGRALAGTRGHQQIQKSRPSHYQAEVPV
                     SYRHVAPDLVFEIGGRLDGVWIDESEVTLDEIKTTTVPLTEITRDNQPLHWAQARAYG
                     YMYAHEHGLSHISIQLTYLNLDTLDILELRDSFTLLELQTFFQDLLRRYAEWARTIRD
                     WHLQRNASIEALAFPFTTYRDGQRQLAIGVFRAIAAGNNLYVQAPTGIGKTVATLFPA
                     LKSLGLSAVQRIFYLTARTVGRDVAEQTLKSLRRKGLRCKVLTLTAKEKCCVLPKPDC
                     RPESCTRARGYYDRVRPALDELFVQDTFDRQMLEQISERHSVCPFEFSLFASLWADVI
                     ICDYNYAFDPRVYLRRFFEPPIEPCAFLVDEAHNLPDRAREMFSADLYKSQFTSLRTE
                     LREAAPAVARAISRVTPFFRLAGKAMQNAVQQTLSFEESESPELANDVTPIQRPAAVS
                     TDTQLILYQMPADFVAAVRSFLKAAEAALVVSPSAPYRELLLELYFKSTAFVRVSETF
                     DDTFVFILDADVLPPVTGGTSRAFTRRDVRTRLFCVDPATRLQEALKRSRSTIFFSAT
                     LLPMNYFFRLFGGQATDKVLKLRSPFAARQLGLFVLPSIRTEFRVREQSHATIAEAIR
                     TIVSSRRGNYLVYFPSYRYLSDVLAAFSNRCPDTRVIVQSAGMDEAARDAFLKTFTSE
                     SDETLVGFAVLGGVFGEGIDLRGERLIGTIIVGVGLPQICLERNLIRERFEQSEKAGF
                     AFAYRFPGMNRVMQAAGRVIRTETDRGVVVLIDERFSRLEYRELFPREWEHARLVPSI
                     FELQRRLESFWQAAPVQAT"
     gene            complement(6193..7887)
                     /locus_tag="PKO06_01125"
     CDS             complement(6193..7887)
                     /locus_tag="PKO06_01125"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNV68268.1"
                     /translation="MDPAAPTDTSVGVLPVLPVGYYLEHFRAVLETVADRYGDLLTEQ
                     EQTFLRRFAELSPDAQKLYVRLTSRRGPLFRSDRLSYAEIGRIDAAARELSACELLAI
                     DHDTWLEEAVALLTVPELKALLQQRNIRTAGLGRMELLACLSRDVAADVTRTLLHEKF
                     HWYAPLCQEVLILFRLLFFGAPEPDLKAFVLSDLGRVQFEKVPLLSARRLFTSRTEID
                     RALLLHELKTRLYELLEDKANPDLPVQVEELIRKVPAADGHPLLLRWRRSCFEVAARF
                     WEREGEPRKALTLYADPETHPARERRARLLLKAGNESEAQRVLAQILSEPRDPAEEEV
                     AIALRGRFLEGRKRTARFNPEVEMLELPGPTGERIEARVLAALRTAGREGFEAENRFW
                     NSLFGIIFWDIIFAPIPRAFGHPFQYGPTDLFTAEFYPARADLIQHRLSEMRSREWLM
                     SRCEEICRTKRHIACALVDWEFVCSIDLPRVVGVVPSDHLGMICERLARNPGGFACGF
                     PDLFLFADDQPGYLLLEVKSPGDRLQDNQRSWMRTFAAHQIPCRLLQVTYPADQVA"
     gene            complement(7910..8695)
                     /locus_tag="PKO06_01130"
     CDS             complement(7910..8695)
                     /locus_tag="PKO06_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68269.1"
                     /translation="MAWKQPEVWFGLLGAAVVSACLLLRMNVRGMTTRGPGWWRRLLV
                     AGLSVLGLSVTPAMTVAEEPQIVSDEPQKQEERIDMQKLAEHAEGKKLLDVWKTAEDI
                     ASGKKGHFPFAQAEKEQLLKDLAAAETLIDELAKKGCYRQEEGELLKVELRTLVKGVQ
                     KMRPTELQQATCYKPMMLVPAVDALDRLETRLPLLEKLGSGTLSPAVAEKLARSIRTD
                     LKMLKNPLPDYYRPTTDPRPRIEAMLKRLPEILEKMGVKPDEK"
BASE COUNT         1834 a         2796 c         2383 g         1797 t
ORIGIN      
        1 ggaacggtcc ggttcaacag tttggaaacc ccgaaggggt ccggtaccgt ctgtttcggt
       61 ggctcaagga cggcaggcgt tttgttgtct gctcggacga accgggggaa gagtgcctcg
      121 aagtgcacac tcaggatggc tcggccccgg tgaaacgcct gcgggccatg cccattggtc
      181 gcccgatcga gctcaaggtc gatccgcaga gcgatcgcgt cgcgctcacc aaccatcgca
      241 acgaactctg gctgatcgat ctcgagaagc aggcccacac ccacattgcc accagtcggc
      301 atgcccctct gcgcggcttc gactggtcac ccgacggaaa atggcttgca tacaatgaat
      361 cgctctcgac acacacgtct gccatctttc tctatgacgt cgtaaagaag aaaaagaccc
      421 aggtgacatt acctgtcctg aacgacgttc agcccacatt cgacccggac ggaaagtatc
      481 tctactttct gagcttccgc gaattcgatc ccgtgtacga tcaactgcac ttcgatctcg
      541 gctttccgag aggaatcaag ccgtatctga tcgtgttgag taacgagaac gtttcaccgt
      601 tcctgccggc tcccaagcca ccggccgaac cacccaagcc tgctgccagc gacaacaaga
      661 ccgctccgaa gtcgaagccc ccggcgccga cacctccggt gacacgcatt gacctcgacg
      721 gcatcaccga tcgcgtgctc gccttcccgg tcaacgacgg gatctacacc cggctcgcgg
      781 caatttccgg caaggtctac ttcggcacgc aaccggtaca gggaaccatc agccagaact
      841 ggcatgacaa gccggcaaac ggcacgatcg agtgttacga tctcgaactg cagaagctcg
      901 acccggtggt cagcggtgtc agcgacttcc gagtatctcc tgatcgcaag tggctgctct
      961 atcgcacggg caatcgtctg cgcctcgtca aggcgggcga gaagcccgat gaagccgcgg
     1021 ctcgcgaagg acccgggcgc aagagcggct ggatcgacct gggacgcatt cagatttcgg
     1081 tcaacccgca ggccgagtgg cggcagatgt accgcgaagc ctggcgcctg cagcgagatc
     1141 atttctggac cgaagatctg tcgcaggtcg attgggaaaa aatctaccga cgctatctcc
     1201 cactgctcga tcgcctcggc acccgtggtg agttttccga cctggtctgg gagatgcagg
     1261 gggaactcgg cacgtcgcat gcctatgaaa tggggggtga ttatcgtctg gctccgaact
     1321 acggattagg gcgtctggcg atcgatacca actgggacga aaagctcaag ggctggaaga
     1381 tcctcgcact ttcgcgcggt gatccatggg acgaacgccg ttcctcgcca ctgctccggc
     1441 ccgggatcaa tgcccgcccc ggagacgcca tcattgccat tggagggcgc gcgctctcag
     1501 cccatctgac acctgacgaa gcgctggtga atcttgctaa tcgcgaagta caagtgacaa
     1561 tcctgtcaaa gagccagaag aagccacgca ccgtgaccgt taaaacgctg gccgacgaaa
     1621 gcgcttcccg atatcgcgac tgggtcgaac agaatcgcgc tttcgtgcac gagaaaagcc
     1681 aggggcgcat cggatatgtt cacatccccg atatggggcc tcgtggctac gccgaattcc
     1741 atcggtcgtt cattgccgaa gtcgatcgtg acggcttgat cgttgatgtc cggttcaacg
     1801 gcggtgggca cgtgtcgcag ctgctgctcg agaagctggc gcgtcggcgg ctggcctatg
     1861 tgcagacacg atggttcggc accgagccct acccggccga gtcggttgcc ggtcccgtga
     1921 tcgcgttgac caacgagaat gccggctccg acggtgatat cttctcgcac tgtttcaagc
     1981 tgctcaagct cggacctctg gtgggcaagc gcacctgggg tggcgtcatc ggcatctggc
     2041 cccggaatca gctcgtcgac gggggtttga cctcacaacc cgagttctcg ttctggttca
     2101 ccgatgtcgg ctggcaggtc gagaactacg gcaccgaccc ggatatcgaa gtcgattttc
     2161 tgccgcagga ccacgccgcc ggacgcgatc cacaactcga acgcacagtc ggcgaaattc
     2221 tgcggttgct gaaacagaaa ccaccacggt atcccgacct cggcaaggga cgccccaaga
     2281 agcccctgcc gaaactgcct cgttgaatct ctcgcagaaa gtgtgcgtgt tttgccagtg
     2341 gccggtatga gaaactcgcc tcgccagcaa aggcaggtgt agaatagcga tatcacatat
     2401 ctctcaacga tggaggttct ttcatgcgca agctcgcgtt tctgttagtg gcaggtctgt
     2461 tgatcgcctt gccggctttc gccgtcttct gcaccacctg cggcgaaaaa ctgcctgact
     2521 cggctcgttt ctgttcgaaa tgcggaggca agatcaccgc cccaacccca gagaaaaaga
     2581 ccactcccgc ggtcaaagag aagccggcag cagtcgagcc ggctcccacg acggaacccg
     2641 cacccgcggc ctccgctcct tcgctggccg gtacattccg cctgaaaacg gacttgtacg
     2701 tgtatgccaa gcgtggcgac gagcacaacg tgctcaaaaa gaatctgttc ttcaaacccc
     2761 gacgcgaccg tatcaaggcc aatgcggaaa tcaagattct cgaagtcgtg ggtgatgcgt
     2821 atctggtcga gcaggtcgcc cccgtcaacg gcaaagggct caagggctgg tgcaccgaat
     2881 cagaactggc tctgcgctcc acctggacca agtaaggact cgcgtcgtct cgacacatga
     2941 ccaacacgca gtcgtcagcg aaaccggcca ggacaaccct ggccggtttt ttcgaacacc
     3001 gggaagagat caagcacagc cgcttcattg ctcgtgcctg ttccgtgaac accccggacg
     3061 aagcgtcagc ctggatctcg cagcatcgag atccagaggc aacgcacaac gcctgggcct
     3121 ggtccatgca cggatctttt cgctttcacg atgatggcga agtctctggc accgccggaa
     3181 aacccatatg gggagccatc gaaaagcagg gcctcgatcg cgtcgtcgtt ctcgtcaccc
     3241 gctactacgg cggaatacaa ctcggtgccg gcggattagc gcgagcctac ggtggagccg
     3301 cggcaacttg tctgcgtctt gctcccaagc gccttctaca gcgagttctg agtgccagtt
     3361 ttcagattcc cttctccgaa cagggaagga cctatcacct gttcgaacgc taccagatcg
     3421 agcgattgtc tgaaaccttc accgatcgcg gcgtcacctt tgaagtccgt ctgtctcccg
     3481 aacggctcga cggattccgc gccgccctgc gcgacacgtt acgcagccct gacgatcatt
     3541 ggatattgat ctctgacgat ctgttctagg acatttatcc attcgtttcc cgctggtcga
     3601 ttccgccggt tcgtgctaag gtatcgtcac caaagcgccg cgttcctgtc attcacgggc
     3661 gctttcctca cccatgactg accgaatctc cactgacgca tccgcgccac aaccatcgat
     3721 tcccgttcac cgaatcgcag tccgcactct ggtggaattt ctgtgtcaga gcggtgatct
     3781 cgtcgcgacc ttcgtcggcg ctggtcgagc tctggctgga acacgcggtc accagcagat
     3841 tcagaaatca cgcccctcac attatcaggc cgaagtcccc gtttcctacc ggcatgtagc
     3901 ccctgatctg gtgttcgaga tcggggggcg ccttgatggc gtctggattg acgaaagcga
     3961 agtcacgctc gacgagatca agacaaccac ggttccgctc accgaaatca cgcgcgacaa
     4021 tcagcccttg cactgggccc aggcacgggc ctatgggtac atgtatgcgc acgagcacgg
     4081 tctgtcgcac atttcgattc agctgaccta tctgaatctc gacacgctcg acattctcga
     4141 actacgagac tcgttcacgc ttctcgaact gcagacattt ttccaagacc tgctgcgacg
     4201 gtatgcggag tgggccagaa ccatccgtga ctggcacctg caacgcaatg cctcgatcga
     4261 agccctcgcg tttccgttca cgacgtatcg cgacggccag cgtcagctcg caatcggcgt
     4321 ttttcgcgcc attgccgcgg gaaacaactt gtacgttcaa gcgccgaccg gcatcggaaa
     4381 aaccgtcgcg actcttttcc ccgcactcaa gtcgctcggg ctgtcggcgg tgcagcgcat
     4441 tttctatctg accgcgcgca cggtcgggcg tgacgtcgcc gaacaaacct tgaaatccct
     4501 gcgtcgcaag ggcttgcgct gcaaagtgtt gacgctgacg gccaaggaaa agtgctgcgt
     4561 gttgccgaaa cccgattgtc gtcccgaatc ctgcacccgt gcccgtggct actacgaccg
     4621 cgttcgtcca gctctcgacg aactgtttgt ccaggatacg ttcgatcgtc agatgcttga
     4681 gcagatatct gagcgccact ctgtctgccc cttcgagttt tctctgtttg cctctctttg
     4741 ggctgatgtc attatctgcg actacaatta tgccttcgat ccgcgcgtct atctgcgacg
     4801 tttcttcgaa ccaccgatcg agccctgcgc atttctcgtt gatgaggctc ataatctgcc
     4861 cgatcgcgcc cgcgagatgt tttcggctga tctgtacaag agccagttca ccagtctgcg
     4921 caccgaactt cgcgaagccg cgccagccgt cgcacgagcg atctcgcgcg tcactccgtt
     4981 ctttcggctc gccggcaaag ccatgcagaa cgcggtgcaa caaacgctca gtttcgaaga
     5041 gtcagaatca cccgaactgg ccaacgatgt gactcccatc cagcgtccgg cagctgtcag
     5101 caccgacacc caactcatac tctatcaaat gcctgccgat ttcgtggccg ccgtccgctc
     5161 ctttctcaag gcggccgagg ccgctctggt cgtttctcct tcagcgccat atcgcgagct
     5221 cctgctggaa ctctacttca aatcaaccgc cttcgtcaga gtctctgaga cattcgatga
     5281 cacgttcgta ttcattctcg atgccgatgt cctccccccc gtgaccggag gtacttcacg
     5341 cgccttcacg cgacgcgacg tccgtacccg actgttctgc gtcgatcctg ccacacgcct
     5401 tcaggaagcg ctgaagcgct cccgaagtac catcttcttt tccgcgactc tgttgcccat
     5461 gaactacttc ttccgcctct tcggcggcca ggctacggac aaggtactga aattgcgctc
     5521 gccattcgcg gctcgacaac tcggactctt cgtcttaccg agcatccgta ccgagtttcg
     5581 ggttcgcgag cagagccatg cgaccatcgc tgaagccatt cgcacgatcg tgtcgagccg
     5641 acgaggcaac tatctcgtct actttccctc gtatcgctat ctgtccgacg tgctcgccgc
     5701 tttctcgaac cgatgccccg atacgcgggt gattgtccag tccgcaggca tggacgaagc
     5761 tgcgcgtgac gcatttctta agacctttac gagcgaaagc gacgagacac tcgtcgggtt
     5821 cgccgtgctg ggaggggtgt tcggcgaagg catcgatctg cgaggcgagc gactcatcgg
     5881 cacgattatc gtcggagtcg gcttgccaca gatctgcctc gaacgcaatc tgatccgcga
     5941 gcgttttgag cagagcgaga aagccggttt cgccttcgcc taccgattcc ccggtatgaa
     6001 tcgtgtgatg caggccgccg gacgagtcat tcgcaccgaa accgaccgcg gagtcgtcgt
     6061 gctcatcgac gaacggtttt cccgcctcga gtaccgcgaa ctctttccgc gtgaatggga
     6121 acatgcccgc ctggttccct cgatcttcga actgcagcgc cgactcgaaa gcttctggca
     6181 ggcagcgccc gttcaggcaa cctgatctgc aggatacgtc acctgcagca gacggcaggg
     6241 aatctggtgc gcggcaaacg tgcgcatcca actgcgctga ttgtcctgca gtcggtcacc
     6301 ggggctcttg acttcgagca gaagatatcc gggctgatca tccgcgaaca gaaacaggtc
     6361 cggaaaaccg cacgcaaaac caccgggatt gcgcgccagt cgttcgcaga tcatacccag
     6421 atggtctgac ggcacgacac cgaccacgcg tggcaaatcg atcgagcaga cgaattccca
     6481 gtcgacaagg gcgcaggcga tgtgtcgttt ggttctgcag atctcttcac atctactcat
     6541 cagccattca cgtgatctca tctcggacag acgatgttga atcagatcgg cacgtgccgg
     6601 gtaaaattcc gccgtgaaca ggtcggtcgg cccatactga aaggggtgac caaaagcccg
     6661 agggatcggt gcaaagataa tgtcccagaa gatgatcccg aacaggctgt tccagaaacg
     6721 gttctcagct tcgaaaccct cacgaccggc cgtgcgtagc gctgcaagca cccgcgcctc
     6781 gatccgctca ccagtcggtc caggaagctc gagcatctcg acttcggggt tgaatcgtgc
     6841 agtgcgcttt cggccttcga gaaaccgacc acgaagagca atggcgacct cttcttcggc
     6901 tggatctcgg ggctcagaga gtatctgtgc cagcacccgc tgcgcctctg attcgtttcc
     6961 ggctttcaac aacagacgtg cccgacgctc gcgggccggg tgtgtctccg ggtcggcata
     7021 gagagtcaaa gctttgcgcg gctccccctc tcgttcccag aaacgagcgg ccacctcgaa
     7081 acacgatctt cgccatctca gcagcaaggg gtgaccatct gccgccggca ctttcctgat
     7141 cagttcctca acctgaaccg gcagatcggg attcgctttg tcctcgagca actcgtacaa
     7201 acgcgttttc aattcatgaa gcagcaaagc acgatcaatc tcagttcgtg aggtgaacaa
     7261 ccggcgcgcc gagagaaggg gaaccttctc gaactgaacc cgcccgaggt cactcaagac
     7321 aaacgccttc agatctggtt ccggcgcacc gaagaacagc agacgaaaga gaatcagcac
     7381 ctcctggcac aggggggcat accaatgaaa cttctcgtgc agcagagtac gagtcacatc
     7441 agcggcaaca tcacgcgaca gacaagccaa cagctccatg cgaccgaggc cggcagtgcg
     7501 aatgttccgt tgttgcagca acgccttcag ttccggtacc gtcaacagcg cgacggcctc
     7561 ctcgagccac gtgtcatggt cgatcgccag caactcgcag gcggaaagtt cccgagcggc
     7621 agcatcgatc cgcccgattt ccgcgtacga aagtctatct gaccgaaaga gcggcccacg
     7681 cctcgatgtc agacgaacat acaatttctg agcatcggga gagagctcgg caaagcggcg
     7741 cagaaacgtc tgttcctgtt cggtcaggag atcaccatac cgatccgcca ccgtctcgag
     7801 cacggcgcga aagtgctcga ggtaataccc aaccggcagg acaggaagaa caccgacact
     7861 ggtgtccgtt ggtgcggcag ggtccatcaa gactacggta gcaatcgtcc tacttctcgt
     7921 ccggcttcac acccattttt tctagtatct caggcagacg tttgagcata gcctcgatac
     7981 gcggacgagg gtcggtcgtt ggacgatagt agtcaggcag aggattcttc aacattttca
     8041 gatcggttct gatcgagcgg gccagcttct ccgcgaccgc cggcgagaga gtgccgcttc
     8101 ccagtttctc aagcagcggc aggcgcgttt ccagacgatc gagcgcatcg acggctggaa
     8161 cgagcatcat cggcttgtaa caggtcgcct gctgtaactc ggtcggccgc atcttctgaa
     8221 cgcccttcac cagggtgcgc agttccactt tcagcagttc cccttcctcc tgccgatagc
     8281 agcccttctt cgccagctca tcgatcagag tttccgccgc cgcgagatct ttcagcagct
     8341 gttccttctc ggcctgcgca aagggaaagt gccccttctt ccccgacgcg atgtcttcag
     8401 cggttttcca gacatcgagc agttttttcc cttcggcgtg ttcagccagc ttttgcatat
     8461 cgatccgctc ctcctgcttc tgcggctcat cgctcacgat ctgcggttct tcagcgacag
     8521 tcatggccgg cgtgacgctg agccccagta ccgacaaacc cgcgacgagc aaacgtcgcc
     8581 accagccggg gccacgcgtc gtcatgccgc gcacattcat tcgcagcagc agacaggcgc
     8641 tgaccaccgc agctccgagc agtccaaacc agacttccgg ttgcttccat gccataaatc
     8701 ctcttcactc cttctctgca attccgtgaa cctgcccgaa gcgtcgaacg gtctgcatca
     8761 ccctcagcgc acgtcgttcg agaggggtgg gcgcagcact caactccact
//
LOCUS       DAORHD010000025         8723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7535_length_8723_cov_3.689086, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000025 DAORHD010000000
VERSION     DAORHD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7535_length_8723_cov_3.689086"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="PKO06_01135"
     CDS             <1..1043
                     /locus_tag="PKO06_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68270.1"
                     /translation="RSPGRRSCSHLMSRMETNLPAPPEKAPTTLNAWMEQRLFFILVA
                     FMGLGWLQADLLAPQKHLVPWLLAGIMWFTSLKSSFADLWRVFRHPRLILLLLLVLHL
                     VLPIPARLLGSLWFSEHGELLAGLVLAAAIPVGVTSVMWSGIAGGNIALALSALTVDT
                     LLSPLILPATLWVFAGAAVTFDASRLVFDLLLMVVLPAVAGILLHDFSGGMIASRIGW
                     LAGPLSKLALGLVLAINLANSKHAVERLPVPLWQLLLCVFLLVIAGLILSMKAAEWTG
                     QRRSVIVVFAYAGGIRNLSAGIVICMRSFPPLTALPVLLGIFFQQPLAALFHRWWRSR
                     RKSSPETEFQLE"
     gene            1079..1537
                     /locus_tag="PKO06_01140"
     CDS             1079..1537
                     /locus_tag="PKO06_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68271.1"
                     /translation="MFFKPEDFTKQWQDQTRQWQEQMEKIFVEKINEMIRNPALLSAV
                     SKSIETSFDSKQKANETIAEFLQKLNVPTRHDTGRIMQYLQQVEARVLDLDEKLDLLL
                     DRVEKLQATSLAQPSIPRAATPAPKNPGQKTVKIAAKKTATRSTSGKGRK"
     gene            1540..2631
                     /locus_tag="PKO06_01145"
     CDS             1540..2631
                     /locus_tag="PKO06_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV68272.1"
                     /translation="MEPTLNPAQIPEEAKKLLDQNAQYAKILMDSYKRTPEEIEALKK
                     TLKICPTPREVIHQECSVKVYRFTPVRKDLHPVPLFIVPSLILRWYILDLITGHSLIE
                     HFVKSGIDTYLIDWGFPGDEHGQLTFDYYIDNFLGRAIRKVMRRTGATQIDLMGQCLG
                     GTIAAAYTSLHQEHIRRFIALTTPVDFENAGLLSIWTDKSRFNVDKIIEYYGNTVPAE
                     FIHGCFQFLDVKATVERYKKLYNNVLDNNFLYYYQALDLWSTDKVPFAGKVFGKFIKG
                     LYQENGLVNKTFEVNGRYVDLAKITCPVLNIAAAFDHVFPERSAKRLNDLVSGPAQYH
                     LMQTGHVTLVVLFPDRLKTYELMTDFLKN"
     gene            2640..3752
                     /locus_tag="PKO06_01150"
     CDS             2640..3752
                     /locus_tag="PKO06_01150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF005262.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNV68273.1"
                     /translation="MIVEKKRFDIPRFPFECNGETIPVAFGYETYGTLNAARDNVVLV
                     CHYFTGQSHAAGKYKPEDAVAGWWDELIGPGKVIDTDQLFVICCDTFCNINANNPAVI
                     TTGPATIDPNTGKPYGMRFPITTLKDMVRSQKMLLDHLGITHLRLIIGPSMGGLQAFL
                     WGKLYPDFMEKIVAVVSTPMVRPWTLMVPNQLGIDAIRLDPRWQNGEYYGGQPPHDGL
                     LLAFKILLISTRTDSWADRNFGRNLADPTGQSIPHPRHSMAGKYLVETEIEKIVLGRM
                     QFFDANSYLYIAKANALYDLRDEGETFEQAMQKIKARTLMIIDDSDLLFTRDQAELAR
                     AHLPDAQCVYYNSLNGHLSCLFDIHLFKEELQSFVS"
     gene            3752..6199
                     /locus_tag="PKO06_01155"
     CDS             3752..6199
                     /locus_tag="PKO06_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68274.1"
                     /translation="MSTLLRRTLRLLVVIGVILGVSSTVSADTATITRRIIAGDVASL
                     PPYAFLQNGTAAGLRIDLLRASAQAVGMEVEFTTASTSMLLERLCDGSIEVIAGFPYS
                     SEDDKAFDFSDPHTLMPYDVLVSPQASVDSYTPGTSLNLILIDQFPVPSEINSGVASW
                     TFLRESSLEAALEQTRLNPGAGILLPRATAYAVQQQHRDKRFRRLPAGFAPTPVCFAV
                     LSGNEQLRFHLNVGLNIIRNNGVYQALQQKWLGPVMEAERDLSRRPWRLSLAIVFALL
                     AMAVLWVITLRRTVKRQTEQVTRTHQDAQETANRFHLLCNSTNIIVAEMTAEGRFLYV
                     NTNFNWAIDTERLSEHALSERCHPDDLAGLNGALHTVRGLPDSRPELTFRVRTRDGDW
                     RWVEASFGSFRTPDGSLHLLMAARDVQERIKLEDQLRESQKMDAIGRLAGGIAHDFNN
                     LLTGILGYANLLKHETTEGSAARDAAETIEGAAKRAGELTKQLLNFARKGNSLTIPVN
                     LHGLIKEITKLLSRTFEKNITLLQKLEATPATVLGDPNQLQQIVLNLAVNAKDAMPEG
                     GTLLFATSTQRMNDSKARSVPGLLPGDYVVFKVTDTGVGIPPEVMDRIFEPFFTTKEP
                     GKGTGMGLALVYSIVKGHGGSVQVTSEPGKGTTFSIYLPLSTQEAADAGKSTFALIPR
                     GSGKVLLVDDEAMIRHLASALLLKLGYECQAFSNPRDALEHFKLRHNDLALVITDMVM
                     ADLDGLELFKAIKALDPQVPVLLTSGYEPGEKVQQALELGAAGFLPKPFKLKELGEAL
                     AKAARKNLPATTDTPTD"
     gene            complement(6236..>8723)
                     /locus_tag="PKO06_01160"
     CDS             complement(6236..>8723)
                     /locus_tag="PKO06_01160"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV68275.1"
                     /translation="LEGGLERAPAEAKLMRAEVELAVVGIAIFRHGNRRQMPAILAAI
                     FQHAADVFLSKADRDLDRLVKLVGRRVMVEKGYGDSLLLQLMREEGIATPAVQFVPHS
                     YSLEELVNDKVDVIHAYATVEPGLLQRRGIATRMISPVDYGIVFYGDMLATRQEYVVA
                     QAEVVAAARRASLKGWDWALKHVDEVASYILSLPGVSDRGIDREFLLFEARNMQSLIR
                     ADIIPLGHINPSRWDHMAHQLVQMGVASDPPAYETWVYRSDQAVHPWWLQTLLLGWLL
                     LLGVLAVLAYSRKQLREIIRRRTATLENEVRQRLQTESVLRASEKQFASLFWENPECL
                     TVTRRLDGKMLLVNKSFATLFESTRDQIEGRTSIELGIWLSPEDREQVIEALKTAGTL
                     RSYETLLRTLQGRVFPALVSVVPYEYQTEDCLLWIILDISQQKRQEEERRRYEMQVQH
                     VQRLESLGIMAGGIAHDFNNLLTGILGNIELLNDELADVPACREPLQDVRAAATRASE
                     LTRQLLAYSGRGRSEDRPLEVNTLIQETVQLLKLSISKKAVLRLDLAAEGLILRGDVS
                     QLRQVLMNLVMNASDALDGRPGEIRIASGLETKNAPFLSEFQPGVNHPAGSYVCIEVR
                     DTGCGIDESLRQKIFEPFFTTKFTGRGLGLAAVLGIVRGHRGALSIESAPGKGTTVRV
                     LLPATNDVVENETRVRVAQEERSMEQQVILVADDEELVRKLAKAMLGKAGYEVIVVED
                     GVFALEELRRRAGKVDLVILDMTMPRLSGDETFRAIQKEFPGMKVIVASGYTEGELVE
                     SFADGKPVGFLGKPFEKDELLSKVTEALAS"
BASE COUNT         1843 a         2700 c         2320 g         1860 t
ORIGIN      
        1 accgttcccc cggccgcaga tcctgcagcc acctgatgag tcgcatggaa acaaatctcc
       61 cggccccgcc cgagaaagcg cccacgacgc tcaacgcctg gatggaacaa cgactgtttt
      121 tcattctggt ggctttcatg ggtctgggtt ggttgcaggc agatctgctg gcaccccaga
      181 aacatctcgt cccctggttg ctggcgggca ttatgtggtt cacctcgctc aagtcgagtt
      241 ttgcagatct gtggcgtgtg ttcagacatc cccgactgat tctgcttctc ctgctcgtgc
      301 tgcatctggt gctgcccatc cccgcacgac tcctgggcag tctttggttc agcgaacatg
      361 gcgaactgct tgccggtctg gtcctcgccg cggcgattcc cgtcggcgtc acctccgtca
      421 tgtggagcgg catcgccggg ggcaacatcg ctctggctct gtcagccctg accgttgaca
      481 cgctgttgag cccgctcatc ctgccggcaa cgctgtgggt ttttgccggc gcggcggtca
      541 cctttgacgc ctcgcgactg gttttcgatc tgctgctgat ggtggtcttg ccggccgtcg
      601 ccgggattct gctgcatgat ttcagtggag gaatgatcgc cagtcgcatc ggctggctgg
      661 ccggccccct gtcgaagctg gcgttaggat tggtactggc catcaatctg gcgaattcga
      721 aacacgccgt cgagcgtctt cccgtgccgc tgtggcagct tctgctctgc gtttttctgc
      781 tggtgatcgc cgggctgatt ctcagcatga aagccgcgga atggacaggc cagcgccgtt
      841 ccgtcatcgt ggtcttcgcc tatgctggcg gaattcgtaa tctcagcgcc ggtatcgtca
      901 tctgcatgcg tagctttcca ccgctgacgg cactgccggt tctcctcggc atctttttcc
      961 agcagcctct ggccgcgctg ttccatcgct ggtggcggtc ccgtcgaaag agttcccctg
     1021 aaacggaatt tcagctagaa tagatttgct tttcagccta tgagacggga ggaacccgat
     1081 gtttttcaaa cccgaggatt tcaccaagca gtggcaggat cagacccgcc aatggcagga
     1141 gcagatggaa aagatcttcg tcgagaagat caacgaaatg attcgcaacc ccgccctgct
     1201 cagcgccgtg agcaagagta ttgaaacgtc gttcgattcg aagcagaaag ccaatgagac
     1261 gatcgccgag ttcctgcaga aactgaacgt tcccacccga cacgacaccg ggcgcatcat
     1321 gcagtatctg cagcaggtgg aagcacgtgt gctcgacctg gatgagaaac tcgacctgtt
     1381 gctggaccgc gtcgagaaac tgcaggccac ctcactcgct cagccttcca ttcctcgcgc
     1441 agcgacgccc gcaccgaaga accctgggca gaaaaccgtg aagattgcgg cgaagaaaac
     1501 cgcgactcgt tcgaccagcg gcaaaggacg gaaataacca tggaacccac actcaatccc
     1561 gcccagatcc ccgaagaagc gaagaaactg ctcgaccaga atgcccagta tgccaagatc
     1621 ctgatggatt cgtacaagcg caccccggag gagatcgaag ccctcaagaa aaccctgaag
     1681 atctgcccca ccccgcgtga ggtcattcac caggaatgct cggtcaaagt ttatcgattc
     1741 acccccgttc gaaaagatct gcaccccgtt cccttattca tcgttccctc gctcattctt
     1801 cgctggtaca ttctcgatct catcacgggc cacagtctca tcgaacactt tgtcaaaagc
     1861 ggtatcgaca cctatctgat cgactggggg ttccccggcg acgaacacgg acagttgacc
     1921 ttcgattact acatcgacaa cttcctgggt cgagcgatcc gcaaggtgat gcgccgcacc
     1981 ggagccacgc agatcgacct gatgggtcag tgtctgggcg gtaccatcgc cgccgcctac
     2041 acctcgctgc accaggagca catccgtcgt ttcatcgctc tcaccacccc ggtcgacttt
     2101 gaaaacgcgg gtctgctgtc gatctggacc gacaagtcgc gtttcaatgt cgacaagatc
     2161 atcgagtatt acggcaacac cgttcccgcc gagttcatcc acggctgctt tcagtttctg
     2221 gatgtcaaag caacagtcga gcgttacaag aagctctaca acaacgttct cgacaacaac
     2281 ttcctgtact attaccaggc gctcgatctc tggagcacgg acaaggttcc gttcgccggc
     2341 aaggtcttcg gcaaattcat caaagggctg tatcaggaga acggattggt caataagacg
     2401 ttcgaggtga acggccgtta cgtcgatctg gcgaagatca cctgcccggt cttgaacatc
     2461 gcggcggcgt tcgatcatgt ctttcctgag cgttcggcca aacgactgaa cgatctggtc
     2521 agcgggcccg cccagtatca cctgatgcag accggtcacg tcacgctcgt ggttctgttc
     2581 cctgaccgtt tgaaaacgta cgagctgatg accgactttt taaagaactg aggctcatca
     2641 tgattgtcga aaagaagcgt tttgacatac cgcgatttcc cttcgagtgt aatggcgaga
     2701 cgattcccgt cgctttcggt tatgagactt atggaacgct gaacgcggcg cgcgacaatg
     2761 tcgtgctggt gtgccactac ttcaccgggc aaagtcatgc cgccggaaag tacaagccgg
     2821 aggacgctgt cgccggctgg tgggacgagt tgatcggtcc gggaaaagtc atcgataccg
     2881 accagttgtt cgtgatctgc tgcgacacgt tctgcaacat caacgccaac aaccccgctg
     2941 ttattaccac cggtccggcg acgatcgacc cgaacaccgg gaaaccgtat gggatgcgtt
     3001 ttccgatcac cacgctcaag gacatggtgc ggtcgcagaa gatgctgctc gaccatctgg
     3061 gcatcaccca tctgcggctg atcatcggcc cctcgatggg aggtctgcag gcgttcctgt
     3121 gggggaaact gtatcccgac ttcatggaaa aaatcgtggc cgtggtttcg accccgatgg
     3181 ttcgtccctg gaccctcatg gttccaaacc agctcggtat cgatgccatt cgcctcgacc
     3241 cccgctggca gaacggagaa tactacggtg gccaaccccc gcatgacggc ctgctgctcg
     3301 cgttcaagat tctgctcatt tcgacccgca ccgattcatg ggctgaccgc aacttcggcc
     3361 gcaatctggc cgatccgacc gggcagagca ttccccatcc gcgtcattcg atggctggca
     3421 agtatctggt agaaacggaa atcgagaaga tcgttctcgg acgcatgcag tttttcgatg
     3481 ccaattccta cctctacatc gccaaagcca atgccctgta tgatttgcga gatgaaggcg
     3541 agaccttcga gcaggccatg cagaagatca aggcccgaac gctgatgatc attgacgatt
     3601 ccgatctgct gttcacccgc gatcaagcag agctggccag ggctcacctg cccgatgcac
     3661 agtgcgtgta ttacaatagt ctgaacggac atctgtcctg tctgttcgat attcatcttt
     3721 tcaaagagga actccaatca ttcgtttcct gatgtctacg ctgctccggc gcaccctccg
     3781 tctcctcgtg gtgatcgggg tgattcttgg ggtcagctca acggtgtcgg ccgataccgc
     3841 gaccatcacc cgacggatca tcgccggcga tgtggcctct ctgcccccct acgcctttct
     3901 tcagaacgga acagcagctg gattgcgcat tgatctgctc cgagcctcgg cccaggccgt
     3961 cggcatggag gtggaattca caacggcttc aaccagtatg cttctcgagc gcttatgcga
     4021 cggatccatc gaagtcattg ccggatttcc gtattcttcg gaagacgaca aggccttcga
     4081 cttttccgat ccgcacaccc tgatgccgta tgatgttctg gtctcgccgc aggcgtcagt
     4141 cgattcctat actccgggca cctcactgaa tctgattctc atcgatcagt tccccgtacc
     4201 cagtgaaatc aactcgggag tcgccagctg gacgtttctc cgtgagtctt ctctggaagc
     4261 ggcattagag caaactcgtc tgaatcccgg ggccggtatc ctgctcccgc gcgccacggc
     4321 ctatgcggtt caacagcagc accgagacaa gcgctttcgc cggcttccgg cgggctttgc
     4381 gccgactccg gtctgttttg cggttctcag cggaaacgag caactgcgtt ttcacctgaa
     4441 cgtcgggctc aacattatcc gcaacaacgg cgtctaccaa gctcttcagc agaagtggct
     4501 cggcccggtc atggaggcgg agcgtgacct cagccgacgc ccgtggcgtc tgtcgctggc
     4561 cattgtcttt gcgcttttgg ccatggccgt gttgtgggtc atcacgttgc ggcgcaccgt
     4621 caagcgtcag acggaacaag tgaccaggac tcaccaggac gctcaggaaa cagccaaccg
     4681 ctttcacctg ctctgtaaca gtaccaatat catcgtcgcc gaaatgacag ctgaaggtcg
     4741 gtttctctat gtcaatacca actttaactg ggccatcgac accgaacgac tttcggaaca
     4801 tgctctgagc gagcgttgtc accccgatga cctcgccggt ctgaacggcg ctctgcatac
     4861 ggtgcgcggt ctgcccgaca gtcgtcctga actgacgttt cgggttcgca cccgggacgg
     4921 agactggcgt tgggtcgaag ccagtttcgg ttccttccgc accccggacg gctcgttgca
     4981 tctgctgatg gccgctcgtg acgttcagga acggatcaag ctggaagatc aactgcgaga
     5041 gtcccagaag atggatgcca ttggccgcct ggcaggcggc attgcgcatg atttcaacaa
     5101 tcttctgacc ggtattctcg ggtatgcaaa cctgctcaag cacgagacca ccgagggcag
     5161 cgctgcccgc gacgccgccg agacgataga aggggcagcc aaacgggccg gggaacttac
     5221 caaacagctt ctgaacttcg cgcgcaaagg caattccctg acgattccgg tcaacctgca
     5281 cgggctgatc aaggaaatca cgaagttgct gagccgcacc ttcgaaaaga acatcacact
     5341 actgcagaag ctcgaagcca ccccggccac cgttctcggc gacccgaatc agttgcagca
     5401 gatcgtactg aacctggcgg tcaatgcgaa agacgccatg cccgaaggcg gcaccctgtt
     5461 gttcgccact tccacccagc gcatgaacga cagcaaggcc cggtccgtac cggggcttct
     5521 gccgggagat tacgtcgtat tcaaggtcac cgacaccgga gtcggaattc ctccagaagt
     5581 catggaccgc attttcgaac cgtttttcac gaccaaggaa ccaggcaagg gaaccggcat
     5641 gggcctggct ctggtgtact cgatcgtcaa gggtcatggc ggtagcgtgc aagtcacgag
     5701 cgaaccggga aagggaacca ccttttccat ctatctcccg ctcagcaccc aggaggcagc
     5761 agacgcagga aaatcgacgt tcgctctgat tccccgaggc tcaggaaaag tccttctcgt
     5821 cgatgatgaa gcgatgattc gtcacctcgc ttccgccctg cttttgaaac tcgggtatga
     5881 gtgccaggcg ttcagcaatc ctcgcgatgc tctcgagcat ttcaaactgc gacacaatga
     5941 tctggcgctg gtcatcaccg atatggtcat ggccgacctg gacggtctcg aactgttcaa
     6001 ggcgatcaag gcccttgacc cgcaggtacc ggtcctgctc acctctggtt atgaacccgg
     6061 agagaaggtg cagcaggccc tcgaactcgg tgccgccggt tttcttccca aaccgttcaa
     6121 attgaaagag ctgggtgagg ctctggcaaa ggcagcgcgc aaaaatctgc ccgccacaac
     6181 cgacacgcct accgactgag ttcgctgcca gaagcgtttt ttgcgggtga agcgatcacg
     6241 aagccagagc ttcagtgact ttgctcagca actcgtcttt ctcgaacggt tttcccagaa
     6301 agccgacggg ctttccgtcg gcaaacgatt cgaccagctc gccttccgtg taaccgctgg
     6361 cgacaatcac cttcattccc ggaaactctt tctggatggc gcgaaaggtt tcgtcaccac
     6421 tcagccgcgg catggtcatg tcgagaatga cgagatcgac ctttccggcg cggcgtcgca
     6481 gttcctcgag ggcaaagacg ccgtcttcga ccacgatcac ctcatacccg gctttaccca
     6541 gcatcgcttt cgcgagtttg cgtaccagct cttcatcgtc ggcaaccaga attacctgtt
     6601 gttccatgga ccgttcctcc tgtgcgaccc gcacccgcgt ctcgttttcg accacatcat
     6661 tcgttgccgg caacagcacc cggacagtcg tgcccttgcc tggcgcgctt tcgatgctca
     6721 gagccccgcg atgaccgcgg acaattccga gaaccgccgc cagcccaagc ccacgccccg
     6781 tgaacttggt ggtgaaaaac ggttcaaaga tcttctgacg caacgattca tcgatgccgc
     6841 accccgtgtc acgcacttcg atacagacat acgatccggc cggatggttc acccccggtt
     6901 gaaactctga tagaaacgga gcgttcttcg tttcgagacc gctcgcgatg cgaatctcac
     6961 cgggtcttcc atcaagagca tcagacgcgt tcatgaccag attcatcagg acctgtcgca
     7021 actgcgacac gtccccacgc agaatcagtc cttctgcggc cagatcgagc cgcagcaccg
     7081 ccttcttcga aatcgacagt ttcagcaact gcacggtctc ctgaatcagc gtgttcactt
     7141 ccagtggtcg atcttcactg cgaccacgac cggaatacgc cagcaactgg cgagtcagtt
     7201 ccgaagcccg tgtcgccgcg gctcgtacat cctgcaatgg ctcgcggcag gccggaacat
     7261 cggccagttc gtcgttcagc agttcgatat ttccgagaat gccggtcaac aggttgttaa
     7321 agtcgtgagc gattccgcct gccatgatgc caaggctttc gagacgctgc acgtgctgca
     7381 cctgcatctc atatcgccgc cgctcctctt cctgcctctt ctgctggctg atatcgagaa
     7441 tgatccataa caggcagtct tctgtctggt actcataggg gaccactgac accagtgccg
     7501 gaaacacgcg cccttgcaac gtgcgcagga gagtctcgta tgatcgtaaa gtcccggcgg
     7561 tcttcagggc ctcaatgacc tgttcccgat cttcgggact gagccagatc cccagttcga
     7621 tcgaggttct gccttcaatc tggtcacgag tgctttcgaa cagcgtcgca aaactcttat
     7681 tgaccagaag catcttccca tcgagtcgac gggtcaccgt caggcactcc gggttttccc
     7741 agaagaggct ggcgaactgt ttctccgagg ctctcaacac cgactcggtc tgcagacgct
     7801 gccgcacctc gttctccagc gttgcggtgc gacgacgaat gatttcgcgc aactgtttgc
     7861 gggagtatgc caataccgcg agcaccccca gcaggagaag ccatccgagc agaagtgtct
     7921 gaagccacca gggatgaacg gcctgatcac ttcgatacac ccaggtctca taggccggag
     7981 ggtcgctggc gacacccatt tgtacaagtt gatgagccat gtgatcccag cgggaagggt
     8041 ttatatgacc caacggaatg atgtcagcgc ggatcagact ctgcatgttc cgtgcttcga
     8101 agagcagaaa ctctctatcg atgcctcggt cagagacacc gggcaaagac agaatgtagg
     8161 aagccacctc atcgacatgc ttcagcgccc agtcccaacc cttcagcgat gctcgacgcg
     8221 ccgcggcaac cacctctgcc tgtgccacga cgtattcctg acgagtggcc agcatatcgc
     8281 cgtaaaaaac gatcccatag tcgacgggtg agatcatgcg ggtcgcaatg ccccgccgct
     8341 gcagtaaccc cggttcaacc gtcgcatagg cgtgaatcac atcgactttg tcgttcacga
     8401 gctcttcaag cgagtaagag tgtggaacga actggacagc gggcgtggcg atcccctcct
     8461 cacgcatgag ttgcagcagc agagaatctc catagccctt ttcgaccatg acacgtcgcc
     8521 ccaccagctt caccaggcga tccagatcgc gatcggcttt cgagagaaac acatcggcgg
     8581 catgctggaa gatcgccgcc agaatcgccg gcatctggcg ccgattgcca tggcgaaaga
     8641 ttgcgatgcc gaccaccgcc agctcaacct ccgcccgcat cagcttcgcc tctgccggag
     8701 ctcgctccaa gccgccctcc agc
//
LOCUS       DAORHD010000026         8625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7659_length_8625_cov_3.415169, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000026 DAORHD010000000
VERSION     DAORHD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8625
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7659_length_8625_cov_3.415169"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKO06_01165"
     CDS             complement(<1..492)
                     /locus_tag="PKO06_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68276.1"
                     /translation="MVEFQDYANRRDPHSEREDSGESPRSRQIKSASGKWNAISADVE
                     ERPKSRLPDLEELAAQGFTSAGRGRLKVDLTTPLEQAFIGGMIVCAIGLLIAVINIGE
                     APGWIPFSLLMGLLSFFMLYRSTDNYYFLDIPRRELMFHHQSIFYNGEYTVAPFKRIA
                     ATTS"
     gene            complement(533..871)
                     /locus_tag="PKO06_01170"
     CDS             complement(533..871)
                     /locus_tag="PKO06_01170"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNV68277.1"
                     /translation="MLSYIASFFLGGLLVFALPKLVGGIECDNYKSAFRLYIIFSIIN
                     FLIKKIFALFIIPFQILTLGLGLILVNGLILGFATDLAPGIRVNSLTSIFKGGVILGV
                     FNLVIGHLLP"
     gene            complement(890..1393)
                     /locus_tag="PKO06_01175"
     CDS             complement(890..1393)
                     /locus_tag="PKO06_01175"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNV68278.1"
                     /translation="MTISEKIGTCWTNRSPTDRAAVVLTSGFGVGFIPIAPATFGSLW
                     GIPLFLLTQGLPILLQVILLLIAFFVAIALADRGEAIARHADPSFVIVDEIVGMWITL
                     FLCWRTDWAALITAFLLFRFFDIVKVYPIRLFEQFRGGLGIVMDDVAAGMVANTVLRL
                     ILVSGIL"
     gene            complement(1353..1958)
                     /gene="pgsA"
                     /locus_tag="PKO06_01180"
     CDS             complement(1353..1958)
                     /gene="pgsA"
                     /locus_tag="PKO06_01180"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNV68279.1"
                     /translation="MTLATQLTMVRIVAIPVFMTAFLWNGTQLQADWGKVAATVVFII
                     AAITDYYDGAVARWYKEETPLGKFMDPIADKLLVSTALVAMVEYRALTHIPSWAAIVI
                     IGREFAVTGLRLICIPKGVLVESTSMAKWKTTAQLTWIITALVFLSVRIGIETYHLHE
                     QSYGFLNWNHGVILALTWVAVILTLVSGYDYFRKNWHLLDQ"
     gene            complement(1955..3280)
                     /gene="rimO"
                     /locus_tag="PKO06_01185"
     CDS             complement(1955..3280)
                     /gene="rimO"
                     /locus_tag="PKO06_01185"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNV68280.1"
                     /translation="MKFHLVSLGCPKNLVDSEGVAADLHRLGWTYTDQPQAADLLFVN
                     TCGFIQEAKEESLAMIMKMLEWKQEKPQLKVCAFGCLVKRYHEEIARDIPELDHLFDF
                     HSLPHLREWLHQAFPSLSRQRGRIPHEQTSTERERTHRLFTAPHVGYLKISEGCSNRC
                     TYCAIPGIRGDFRSKPLDAVLADATHLVETGAREIIVVAQDTTRYGTDLPGSNHLPEL
                     LQKLSNLPRVRWIRLQYLHPARLSEPVIEQLFSIPKVLPYFDIPFQHVADELLERMNR
                     GVTQDHLENLVRVIRRLFPESVLRSTFIVGFPGETQTHFEQLLEFIEDLPFDRIGAFP
                     YSPEDGTPASRLRPRVPKKVARERLDELMTLQQMLINERNQSWVGKTLEVIVDRLEEG
                     RAHGRTFGDAPEVDNEVHFDVSKRVAPGDFLRVKLTSADAYDFEGELIA"
     gene            complement(3294..7904)
                     /locus_tag="PKO06_01190"
     CDS             complement(3294..7904)
                     /locus_tag="PKO06_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68281.1"
                     /translation="MVSRWEILLEKGQTYFEMGEHEKALQALGKVIDEVPQCIDAIRM
                     KAQAHLSLGQTEPAIQAFEKIIEIDPTDSAAYGHLGQLLVNAGNQNRAIEIMKKAREL
                     NPRDTKLIHEMARLELVRNQFEAAEDLFRQILAIDPSYSAAALELARLAIARDDVSGA
                     IDLYQKALLEDPDNAQLHFELGEAYNFSGQYNEALMEYKKAEYLDPNNQEVLFRIGKD
                     LESKGKLEEALQKYEAVKEIEKNNLEVHYRCGKLYQRMEQIDKAISEFKTVAELKPDW
                     LEIRYELAKLYEILGNYEEQIKEFEAIAQVDANSLEIFMQLATAYENSGNLEKALGFW
                     HKAFLADPENPTFAEKVYELVLQGGQAEPVIDALETLSIVRADDTDLLLILARTYRDR
                     GMMDEANAYYGRIIEIDPKRADIRSELRAVYRSKGMVNEEIDSITLLLESDPDNVELW
                     KDLELLQSQSGRKRELVTTLRNLMRLDPSPAWAARIGQAYSDLGQVDEGLTYLRRALE
                     LNPSDPTPHYTLVTLYKEKGQTERVIESYKAILQIEPNNVNVHLELGDLFKQLGRLEE
                     QVDSLRSAISLGAQDARLFLELGSVLQQLKRIDEAKQVIRQAKELAPDDLSISRRLIE
                     IYVGNNDHEAAVQELETLRQRDPENLDNYLTLSRIYQGRSMFAEALEVLNQARTIAPQ
                     SLEISIQMGEVYNLQENYAEAIALMKETRTLAPLDPRISGVIAEAYAKSGEHESAIME
                     LKQLISSDAGNTLARIQLGRVYTIANMLDQATMAFKDVIRDVPQDPIAHVELGKIYVT
                     MGHNFEDQSMIDNALSEFTEAIKVDPKCPDAHLQIGLVLKERGRVDEALAEFEKVLRV
                     DPLNAAAKVQHEELSATKLESMAGEGINQARIFNERGMFKNAVLEYQNVLKITPTNYL
                     AWYELGKVYLEQKQFAEALEALSKAKEIKQDYLETYVALAQAYLAQNEPDNASMELQE
                     LLGIEPNHLYGMIMLGEIFQTKGWTDEAIERFEAAFAIDPTQLTPVLQLGTLYREKGD
                     FDTAKKWFAKVIDLDAGNAAATDFFANLTASQRQNDIVEALQRAHEAEAKGDVDAAIM
                     FFDSVIDLDGKNLEALYNLGRLYEQKHMIDSAAFEYERALQVDAEGKHKDLTVRLGDL
                     YTRQGKIVESVEVLRQAKQFFPDNTNVRIQFITQVKFKELNKFSTPDELADIVQEIRA
                     AAEKNQSANDWLELGLFVSKGLDGTLSEDDAINQAISYFEKVLEIDSGNIFAMVELAK
                     VYFRKNLFEQVEATYKKVLELDPENAQVHRKLSELYAQQKRFDLALSEIRQLMEIEPA
                     NGDHSTRLIDLYKEMYAKDENRAKQYQQIIAEMKSKVEANPKDAMMAYALGYSYIMLS
                     AGYTPSEEEMQWSVYYFKQANALDPNNLWPYWGLKLVYNKQSLSGKHMYDEAIAICKK
                     ALKIDENNSRAHYELGEAYNENYDVNMKNQALEEYKRAVQLDPNFVEAHFRLASILRV
                     KNMFDKAVEEYNKVIELDPTSPLAKDAKRSLIHIERSRGEAA"
     gene            complement(8017..>8625)
                     /locus_tag="PKO06_01195"
     CDS             complement(8017..>8625)
                     /locus_tag="PKO06_01195"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68282.1"
                     /translation="CIYALGSLYWEKKFYDKMVTLFKKAISLYPQNTRAHFELGKAFR
                     RKFKIAEAMDEFKKVINIDSGYAEVYFELGSLHLENGETAEALECLKKATSLNPANGE
                     AFFLLGKVYEENGDRDKATHAYQQARSKMTDNVEILVKLAADYLDKKKYEEALTELKK
                     AAAMDGVTADVYVMMSKAYRALGMKSEALAAIEKAETFEEGK"
BASE COUNT         1758 a         2327 c         2461 g         2079 t
ORIGIN      
        1 cgacgttgtc gcggcgatgc gtttgaatgg ggctaccgtg tattcaccgt tgtagaagat
       61 cgattgatga tggaacatca gttcgcgacg cggaatgtcg agaaagtagt agttgtcggt
      121 cgatcgatac aacatgaaaa acgagagaag gcccatcaag agcgaaaacg ggatccatcc
      181 cggagcttca ccgatgttga tgacggcgat cagcagaccg atggcacaga caatcatgcc
      241 gccgatgaat gcctgttcga gaggcgtggt cagatcgact ttcagacgtc ctctgcctgc
      301 cgacgtaaac ccctgagcgg caagctcttc aaggtcggga agccgcgatt tcggtctttc
      361 ctccacgtcg gcggagatcg cattccactt gcctgaagcg gattttatct gacgggagcg
      421 gggactctct ccagaatcct cgcgttctga atggggatcg cgtcggttgg cgtagtcttg
      481 aaattctacc attactcgct cctctcgctt ctgcagtggt caggttggct ggtcagggca
      541 gaagatgccc gatgacgagg ttgaataccc ccagaataac accgccttta aaaatactgg
      601 taagactgtt tacacgaata ccgggtgcca ggtcggtggc gaagcctaat atcaagccat
      661 tcaccaggat cagaccaagc ccgagggtga gaatctgaaa tggaattatg aacagagcaa
      721 agatcttttt tatcagaaaa ttaatgatcg aaaaaatgat ataaagtcga aaggcgcttt
      781 tgtagttatc gcactcgatg ccgccaacca gcttgggaag cgcgaaaacg aggagaccgc
      841 caagaaagaa gctcgcgata taggaaagca cgtgagtgag cccctgttgt cagagaattc
      901 cgctgaccag aatcaaccgc aacaccgtat ttgccaccat gccggcggcg acatcgtcca
      961 tgacgatccc gagacctccg cgaaactgct cgaacagacg gatcggatag actttgacga
     1021 tatcgaaaaa tcgaaacagg agaaaagcgg tgatgagtgc cgcccagtcg gtgcgccagc
     1081 acaagaacag ggtgatccac atgccgacga tctcgtcgac gatcacgaat gaagggtcgg
     1141 catgacgggc aatggcttcg cctcggtcgg ccagagcgat ggccacaaag aaagcgatga
     1201 gcagaaggat gacctgaagc agaatcggaa gcccctgtgt cagcaggaag agagggatcc
     1261 cccacaggct gccgaaggtc gccggcgcga tcgggataaa gccgacaccg aatccggaag
     1321 tcaggacgac ggcggctcga tcggtagggg atctattggt ccagcaagtg ccaatttttt
     1381 ctgaaatagt catacccaga aaccagagtc agaatgacgg ccacccaggt gagagcaagg
     1441 atgacgccgt ggttccagtt caggaacccg tagctctgct catgcagatg gtaagtctcg
     1501 atgccgatgc gcaccgagag gaacaccagc gcggtgatga tccaggtcag ttgtgcggtg
     1561 gtcttccact ttgccatcga ggtcgattca accagcactc ctttgggaat gcagatcaac
     1621 cgcagcccgg tgaccgcaaa ctcccgacca atgatcacga tggccgccca cgagggaatg
     1681 tgcgtcaggg cgcggtactc gaccatggcg accagagcgg tcgaaaccag cagtttgtcg
     1741 gcaatcgggt ccatgaactt gccgagcggg gtttcttctt tgtaccagcg ggccacggcg
     1801 ccatcatagt agtccgtaat ggcggcgatg atgaagacga cggtcgcagc gacctttccc
     1861 cagtctgcct gcagctgggt tccgttccag agaaaggcgg tcatgaacac gggaatggcc
     1921 acgattcgca ccatggtcag ctgtgtcgcc agagtcacgc gatcagttct ccttcgaaat
     1981 catacgcatc tgccgaggtc agttttaccc gaagaaagtc accgggggca actcttttcg
     2041 agacgtcgaa gtgcacttcg ttgtcgactt cgggggcgtc gccgaaggtt cgaccgtgcg
     2101 cgcgcccttc ttcgaggcgg tcgacgatga cctcgagtgt ttttcccacc caggactggt
     2161 tgcgctcgtt gatcagcatc tgctgcaggg tcatcagttc atcgaggcgt tcgcgcgcga
     2221 ccttcttcgg gacgcgtggg cgcaggcggc ttgccggagt gccgtcttca ggggaatagg
     2281 gaaacgcccc gatacggtca aacggcagat cttcgataaa ctcgagcagt tgttcgaaat
     2341 gggtctgcgt ctcgccggga aacccgacga tgaaggttga acgcagtacg gattcgggaa
     2401 acagtcggcg aatgactcgc accagatttt cgagatggtc ctgggtgacg ccgcggttca
     2461 tgcgctctaa tagctcatct gcgacatgct gaaacggaat gtcgaaatac ggcaacacct
     2521 tgggaatgga aaacagttgt tcgatcacgg gctctgaaag acgggccgga tgcagatact
     2581 gcaggcggat ccagcggacg cgcggcaggt ttgagagctt ctgcagaagc tcgggcagat
     2641 ggttcgatcc aggcagatcg gtgccatagc gcgtcgtgtc ctgcgccacc acgatgatct
     2701 cgcgggctcc tgtttccacc aggtgtgtcg cgtcggcaag cacggcatcg aggggtttcg
     2761 aacgaaagtc gccacggatg cccgggatgg cgcagtaggt gcaacggtta ctgcagccct
     2821 cggaaatctt gagataaccg acatgcggcg cagtgaacag ccgatgggtc cgttcccgtt
     2881 cggtcgatgt ttgttcgtgg ggaatgcgcc cacgctgtcg cgacagtgat ggaaacgcct
     2941 gatgcagcca ttcacgcaga tgaggcagcg aatgaaaatc gaagagatgg tcgagttcag
     3001 gaatatcgcg agcgatctcc tcgtggtatc gtttgaccag gcagccaaag gcgcagacct
     3061 tgagctgcgg cttctcttgc ttccattcga gcatcttcat gatcatggcg agcgattctt
     3121 ctttggcttc ctgaatgaag ccgcacgtgt tgacgaaaag cagatcggcc gcttgtggtt
     3181 ggtcggtgta ggtccagccc aggcgatgca ggtcagcggc cacgccttcg gaatcaacca
     3241 gattcttcgg gcagccgagc gagaccagat gaaacttcat gcgtttcgag gcttcatgcc
     3301 gcctcgccgc gtgaccgttc gatatgaatc agcgagcgct tggcgtcttt ggcgagcgga
     3361 ctggtcgggt cgagttcgat gaccttgttg tactcttcga cggctttgtc gaacatgttc
     3421 ttcacgcgca gaatactggc cagacggaag tgcgcttcga cgaagttcgg atcgagctgc
     3481 acggcgcgtt tgtactcttc gagtgcctgg tttttcatgt tgacatcata gttttcgttg
     3541 taggcttcgc cgagttcata gtgtgcgcgt gagttgtttt cgtcgatctt caacgctttc
     3601 ttgcagatgg cgattgcttc atcatacatg tgcttcccgg agagggattg tttgttgtag
     3661 acgagtttca aaccccagta gggccacagg ttgttcggat cgagcgcgtt cgcttgtttg
     3721 aagtagtaca ccgaccactg catttcttct tccgaggggg tgtaaccagc cgacagcatg
     3781 atgtacgagt agccaagggc atacgccatc atcgcatcct tgggattcgc ctcgaccttg
     3841 ctcttcatct cggcaatgat ctgttgatac tgtttggcgc ggttctcgtc cttggcatac
     3901 atttctttgt acaagtcgat caggcgggta ctgtggtcgc cattggccgg ctcgatttcc
     3961 atgagctgtc gaatctcgga aagggccagg tcgaaacgtt tctgctgcgc atacaactcg
     4021 ctgagcttgc gatgaacctg cgcattttcg ggatcgagtt cgagcacttt cttgtaggtt
     4081 gcctcgacct gttcaaacag attcttgcgg aaatacacct tcgccagttc aaccatggca
     4141 aagatattgc ctgaatcgat ttccagcact ttttcaaagt agctgatcgc ctgattgatg
     4201 gcgtcgtctt cggagagagt gccgtcaaga ccctttgaga cgaaaagtcc cagttcaagc
     4261 cagtcattcg ctgactggtt cttctctgcc gcggcacgaa tctcctgaac gatatcggcc
     4321 agttcatcgg gagtcgagaa cttgttgagt tccttgaact tcacctgggt gatgaactga
     4381 atgcgaacgt tcgtgttgtc ggggaaaaac tgcttcgcct gccgcagcac ttcgaccgac
     4441 tccacgattt ttccctgccg ggtgtacaag tcgcccagcc gcacggtgag gtccttgtgc
     4501 ttgccttcgg cgtcgacttg gagtgcgcgt tcgtactcga atgctgcgct gtcgatcatg
     4561 tgcttctgtt catacagacg tccgagattg tacagggcct cgagattctt gccgtcgaga
     4621 tcgatgacgc tgtcgaaaaa catgatcgcc gcgtcgacat cacctttggc ttcggcttca
     4681 tgagcgcgct gtagagcttc gacgatatcg ttctgacgct gcgaggcggt caggttcgcg
     4741 aaaaagtccg ttgcggccgc gttgccggca tcgagatcga tgactttggc aaaccacttc
     4801 ttggcggtgt cgaaatcgcc cttctcgcga tagagcgtcc caagctgcaa tacgggggtt
     4861 aactgcgtgg gatcgatggc aaacgcggct tcgaatctct cgatggcctc gtctgtccac
     4921 cccttggtct ggaaaatctc gcccagcatg atcatgccat acagatggtt gggctcgatt
     4981 cccagcagct cctgcagttc catcgaagcg ttatccggtt cattctgcgc gaggtacgcc
     5041 tgagccagag caacataggt ttccaggtaa tcctgcttga tctctttggc tttactcaac
     5101 gcctcgaggg cttcggcaaa ctgcttctgt tcgagataga ccttgccgag ttcgtaccag
     5161 gcgagataat ttgtcggcgt gatcttgagc acgttctgat attcgagaac ggcattcttg
     5221 aacatgcccc gttcgttgaa gatgcgggcc tggttgatcc cttctcccgc catcgattca
     5281 agtttggtcg ccgaaagctc ttcgtgctgc actttggctg ccgcattcag cggatcgacg
     5341 cgcaggactt tctcgaactc cgccagtgcc tcatcgactc ggccgcgctc cttgagtacc
     5401 aatccgatct gcagatgggc gtcagggcac ttgggatcga ccttgatcgc ttcggtgaac
     5461 tcggaaagag cgttgtcgat catgctctga tcttcgaagt tgtgtcccat ggtcacgtag
     5521 atctttccca gttccacgtg tgcgatcggg tcctgcggca catcgcggat cacgtccttg
     5581 aaggccatcg ttgcctgatc gagcatgttg gcaatcgtat acacgcgccc gagttgaatg
     5641 cgagccagcg tgttgccggc atcactcgag atcaactgtt tcagttccat tatggctgat
     5701 tcgtgctcgc ccgacttcgc ataggcttcg gcgataaccc cggaaatgcg tgggtcgagc
     5761 ggcgccaggg tgcgggtttc cttcatcagg gcgatggctt ccgcgtagtt ttcctgcaga
     5821 ttgtagacct ctcccatctg aatcgagatc tcgagactct gcggcgcgat cgtgcgagcc
     5881 tggttcagaa cttccagcgc ttcggcgaac atgctgcgac cctgatagat tcgtgacagg
     5941 gtcagatagt tgtcgaggtt ttccgggtcg cgttggcgca gagtttcgag ttcctgcacg
     6001 gcggcttcgt gatcgttgtt gccgacgtag atctcgatga gccgtcgcga aatcgagaga
     6061 tcgtccggcg caagttcctt ggcctgacga atcacctgtt tggcctcgtc gattcgtttc
     6121 agttgctgca ggacagagcc gagctcgaga aagagtcgcg catcctgtgc gccgagcgaa
     6181 atcgcagagc gcagagaatc gacctgttcc tccaggcgtc cgagttgctt gaacagatca
     6241 cccagttcca gatgcacatt gacgttattg ggttcgatct gcagaatcgc cttgtaagat
     6301 tcgatcactc tctctgtctg gcctttttcc ttgtagagcg tcaccagcgt gtagtgcggg
     6361 gtcggatctg atggattcag ttcgagtgcc cggcgcagat aggtgagacc ttcgtcgacc
     6421 tgtcccaggt cgctgtaagc ctgtccaata cgggctgccc aggcgggcga cggatcgagc
     6481 cgcatcaggt tgcgcagggt cgtgacgagt tcccgtttgc gcccgctctg gctctgcaac
     6541 agttcaaggt ctttccataa ctcgacattg tccgggtcac tttcgagcag aagggtgatg
     6601 gaatcaatct cttcgttgac catgccttta ctgcgataca ccgcgcgcag ttctgagcgg
     6661 atgtcagcgc gcttgggatc gatctcaatg atgcgcccgt aataggcgtt ggcttcatcc
     6721 atcatgccgc ggtcccggta ggttcgggcc aaaatcagca acagatcggt gtcgtcggct
     6781 cgaacgatcg agagagtttc tagggcgtcg atgaccggtt cagcctgacc gccctgcagg
     6841 accagttcat agactttttc ggcgaaggtc gggttttccg gatcggcaag aaacgcctta
     6901 tgccagaatc cgagcgcctt ctcgagattg ccggagttct catacgcggt agccaattgc
     6961 atgaagatct caagcgagtt ggcatcgacc tgcgcgatgg cctcaaactc cttgatctgt
     7021 tcttcgtagt tgccgaggat ctcatagagt ttggccagtt cgtagcgaat ctcgagccag
     7081 tcgggtttca gttcggcgac agtcttgaac tcgctgatgg ccttgtcgat ctgttccatg
     7141 cgctggtaca acttgccgca gcgatagtgc acctcgagat tgtttttctc gatttccttg
     7201 accgcttcat acttctgcag ggcttcctcg agcttgccct tcgactccag atcctttccg
     7261 atgcgaaaca aaacttcctg gttgttcggg tcgagatact cggccttctt atactccatc
     7321 agcgcttcgt tatactggcc gctgaagttg tacgcttcgc ccagttcgaa atgcagctgc
     7381 gcgttatcgg ggtcttcaag taaagccttc tggtacaggt cgatggcacc ggaaacatcg
     7441 tcgcgggcga ttgcgagccg cgcgagttcg agagccgcgg cggagtagga tggatcaatg
     7501 gccagaatct gtcggaacag gtcttcggcg gcttcgaact ggttccgtac cagctcgagt
     7561 cgcgccattt cgtgaatcag cttggtgtct cgggggttga gttcccgagc tttcttcatg
     7621 atttcaatgg cgcggttctg gtttcccgcg ttcaccagga gttggccgag gtgaccgtag
     7681 gcggcggagt cggtcggatc gatctcgata atcttctcga aggcctgaat ggccggttcc
     7741 gtttgcccga gggaaagatg ggcctgggcc ttcatgcgaa tggcatcaat gcattgcgga
     7801 acctcatcga tgaccttgcc caatgcctga agagctttct cgtgctctcc catttcaaaa
     7861 tacgtctgcc ccttctcgag cagaatttcc catcggctta ccatgtcgtt tgtctcctta
     7921 cgccttcggt cgggtctccg atcgttttct gtcgccgggt cccgcccctt cgtgtgtgac
     7981 cattcccgtg aatctgtggc tgcgcgttgc ttggtgctac ttgccctcct cgaacgtttc
     8041 ggccttttcg atggcggcca gtgcttcgga cttcatgccc agtgctcgat aggccttgct
     8101 catcatgacg tagacatcgg cggtgacccc atccattgcg gcggctttct tcagttcggt
     8161 cagcgcttcc tcgtacttct tcttgtccag ataatccgcg gcgagcttga ccagaatctc
     8221 gacgttgtcg gtcatcttcg aacgagcctg ctgataggcg tgcgtggctt tgtcgcgatc
     8281 cccattctct tcatagacct tcccgagcag gaagaaggcc tctccgttcg ccggattcag
     8341 cgatgtcgct ttcttcaggc attccagggc ttcggcggtt tcgccgtttt ccaggtgcag
     8401 gcttcccagt tcgaagtaga cttccgcata cccggaatca atgttgatga ctttcttgaa
     8461 ctcatccatg gcctcggcga tcttgaactt gcgtcggaag gccttgccga gttcgaagtg
     8521 ggcgcgggta ttctgcgggt agagcgagat cgccttcttg aacaacgtga ccatcttgtc
     8581 gtagaatttc ttctcccagt acagcgaccc aagcgcataa atgca
//
LOCUS       DAORHD010000027         8489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_7876_length_8489_cov_3.542447, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000027 DAORHD010000000
VERSION     DAORHD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7876_length_8489_cov_3.542447"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1941)
                     /locus_tag="PKO06_01200"
     CDS             complement(<1..1941)
                     /locus_tag="PKO06_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV68283.1"
                     /translation="MMFRSAVIRRFRAAPLVVAFLLLGLMGCGLREPQTGSLSGRVFN
                     VKGEIVENAKVYSLFNEVEQVYTGNDGSFYLSELPSGRNTIVITHPTYRIESRVVEIP
                     PSTNVAYDEIKLDWATSSQQLSALVIERTASTTADISWKTYKPATTILEWGLTQSYGN
                     TYTEREAVTDHRYTITGLAPDTVYHVRAKLFDDNNNAWFSYDMPFKTTQGPIPAAPVD
                     VRIQPLAAFGVVDLEWDLSTSTAVVGYRVWRREKDTDWTLLTTDLLDRNTRTYADVGA
                     RGGRFYEYAVEAWSLESAVSPRVYSRRVFMPGYINDLVTLTASDSPVLLEADLIVGLA
                     GNLQVGPGVEFRVAASDAFSLGTDRTRVEMLVQGRVTLAGTAADPVRFTPADSSGGRT
                     HWAGIVLQNGGTGFSEIGFVQLSGCTPYALWAKGTTPNLHDISVRYAQGGMYFEDIRA
                     LPTLTGCRFDDIASSALTVVNCRRFDVRDAEITESTIGMVFQAGTTEDYVTVRECRIE
                     ALQEGIRGAFQRSVLKNLLILVPDGIGIRVQSVPLSQNYIDHVTVDADVGIQLENGVP
                     LIENSIIVNTLQEGTVGVRSLTAGNPQFDYINVFGFAAPYENGSGGVGARTEQPAFLG
                     GNPYNYELQLTSMLKRTDRYGQE"
     gene            complement(1928..3928)
                     /locus_tag="PKO06_01205"
     CDS             complement(1928..3928)
                     /locus_tag="PKO06_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNV68284.1"
                     /translation="MIKHRGVFVLCLVVIVGAMLGCLETKQQTPGGLRGIVVDVNGTR
                     LSGVRITVTSASTITASDGTWGLDGLTPAIVTVLAEREGFAAASRQVEVLAGVITENV
                     ILTMEDEGFLYGLSILDVTSSAATIRFYTRGLARTYVQYGPNTLYESKTEMIATSTTA
                     HSFTLTNLTPASTYYIQAVASDTLGHIIYSEGKSFTTAYTQRGDPPLNLKVEKDPYSD
                     AIIVTWSADTGTDLAGYRLYRAMTPQGPFESVDSTLLLSTRYTDTLVLPGQVVFYRVT
                     RVANTNEESPPSQIVSFLLPGITRGNIEWRPEFGPLTLTGDLTIRQDSTLTILPGVQV
                     GVTASDQWDLTSGSDKKVEIKVQGALVVEGNAAAPVSMTSLSAAPRAGDWVGIGFYTT
                     ANLYVSRIDYLNLAFAQYGVHGITGAPPISNSRFANCSAAGVRCIAARAPVSIANSIA
                     DTCASGFLIASNTTQEISIATCTAVRCFYGIVARDNKRATVVGNKVQFWNVTGMDIGN
                     ATTSSFIGRNIIGPGGTGTGIVVRGRDEIRRNTIQAQIGIEIRDSAQATLRSNLILAD
                     QRKGAIGVLYNSADAYVPASHSITFNDIWDLPSTSSNRYIDIRGAVLPGVNNDRYLDP
                     SFEGGTPFVEYPNASFTYRPAPGSRLDREGYGFEDIGAYDVP"
     gene            4023..5228
                     /locus_tag="PKO06_01210"
     CDS             4023..5228
                     /locus_tag="PKO06_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013238779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV68285.1"
                     /translation="MNISQRAASMQASPIRKLIPLANAARKRGVTVYPLNIGQPDIHT
                     PEPVREAIRGFDQKVLAYSPSPGEDYLLEAFSQYYRKNNIPLDPAEIIVTTGGSEAMY
                     FAFLAICDPGDEVLVFEPFYTNYNGMAHQTGVTLKALRTVAEDGFQLPPIEVIKKAIT
                     PRTRAILICNPNNPTGTVYTRQTLEALAHVAKEHKVYLLADEVYREFAYDGLVHTSIL
                     HLPETYDNAILIDSISKRYSACGARIGVIASKNKDVMSACLKLAQARLSSPTVEQWAA
                     RAGLLMDPSYFRPILEEYQRRRDAVMEGFAKIPGCVCKTPKGAFYVIAKLPIQNAEAF
                     ASWLLTDHSYNNETLLVAPAAGFYVTAGLGKDEIRISYCLEVDKLKRAMTTLAEGVKR
                     FQELEKTKH"
     gene            5346..5801
                     /gene="dut"
                     /locus_tag="PKO06_01215"
     CDS             5346..5801
                     /gene="dut"
                     /locus_tag="PKO06_01215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNV68286.1"
                     /translation="MSDTPAPLQIRLQPAGTWPWQVPARATTGASGYDLCASVKGEIG
                     PGKVVAVPTGIKIEIPAGYEGQVRPRSGLAAKHGVTVLNAPGTIDSDYRGEVKVILIN
                     HGEEPFRYEPGMRIAQLVFAKVQEAEFTVVDQIVDSARGEGGFGSTGTF"
     gene            5877..6626
                     /gene="dapB"
                     /locus_tag="PKO06_01220"
     CDS             5877..6626
                     /gene="dapB"
                     /locus_tag="PKO06_01220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNV68287.1"
                     /translation="MSKRRVCVIGCLGKMGQVTCRGLLSTADYEVAAAVDVSHGGEDL
                     GELIHMGHLGVTIADNLQSVLAQEKIDVAIDFTNPDSVVSNVAMCLQARVPVLVGTTG
                     WSEEDLEKVRLLSNKMKTPVLVAPNFAIGAVLMMEFAKKAAKYMPHVEIIEMHNPNKL
                     DKPSGTARLTRELMLKSVGRTDRDPEELIPIHSVRLPGMVAHQEVIFGEVGQLLKIRH
                     DSMQRESFVPGILLGLKQLFHTEGLKIGLEL"
     gene            6730..7839
                     /locus_tag="PKO06_01225"
     CDS             6730..7839
                     /locus_tag="PKO06_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68288.1"
                     /translation="MKVLHFFVKDLGGEFPSDRFPIVATSFGEVVPEEIANVYSGYFT
                     ASGLAPVRIRLRMEDISVGSTKAVLRVDLKLFAFGVVILEYLIEPTSPVADPNQLFHA
                     RSVERASGETVTLNALWQADMALLRDRLYRDIKREYDDPAFTDHFHLFIDHEKRPEEE
                     AMAILLPGEESYSTPIKRRIVQRIQLWEESESCYIMGSRGYLGTALNPFDIVNFMQLA
                     RMQLYELKVYDHILDKSIEDTYAFLDKLPQTNSVLPFSWLHREFQEQVQHATKLLAVR
                     MDLVDLVKDITNTTKITDDPYFELVYRNLMDAFRVREWFDSVKDKIEEVEASQKMILD
                     RLDMVRSTTLEMTIIALIAVEIIVPFGHWIMSFVR"
     gene            complement(7969..>8489)
                     /locus_tag="PKO06_01230"
     CDS             complement(7969..>8489)
                     /locus_tag="PKO06_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I secretion C-terminal target
                     domain-containing protein"
                     /protein_id="HNV68289.1"
                     /translation="LRDGIDTITDFVPGTDRLDLSALLASLGISRAVARSGGYVQLVD
                     TNSGVSVQINADGSAGAGVLRPLVTLRGRTAAQIDFATRGVVEHAGNLGLHQVESVGQ
                     IEQFDILAEHLGVGVLDVLNHAQAEFRIGLGVIQGGIGNDHGRLPPVKNLNFTISIGV
                     RGNAMKNFFSCA"
BASE COUNT         1806 a         2525 c         2480 g         1678 t
ORIGIN      
        1 ttcctgaccg taccggtcgg ttcgcttcaa catcgatgtc agctgcagtt catagttata
       61 cgggtttccg ccgagaaacg cgggctgttc ggtacgtgcc ccgacgccac cgctcccgtt
      121 ttcatacggc gcggcaaaac cgaacacgtt gatatagtca aactgagggt ttccggcggt
      181 gagagatcgt acgcccacgg tgccttcctg taacgtgttt acaatgatgc tgttctcgat
      241 cagcggaacg ccgttctcca gctggatacc aacatcggca tcgacggtca cgtgatcgat
      301 gtagttctgg ctgagcggaa cggactgaac ccgaattcca atgccgtccg gaacgagaat
      361 cagcaggttc ttgagcaccg aacgctgaaa ggctccacga atgccttcct gcagggcctc
      421 gatgcgacac tcacgcacgg tcacgtagtc ctcagtcgtt cccgcctgaa acaccatgcc
      481 gatcgtcgac tcggtgatct cggcgtcacg cacatcgaag cggcggcagt tgaccaccgt
      541 cagcgcagac gaggcgatgt cgtcgaaccg acacccggtc aaggtcggca gagcccgaat
      601 gtcttcgaag tacatgccgc cttgcgcgta acgcacagaa atgtcatgaa ggttcggcgt
      661 cgtacccttg gcccagagcg catagggagt gcatcccgaa agctggacaa agccgatctc
      721 ggaaaaaccc gtgccgccgt tctgcaacac gattcccgcc cagtgagtgc gcccgcctga
      781 actgtctgcc ggggtaaagc gcaccggatc agcggctgtg ccggcgagag tcacccgccc
      841 ctgcaccagc atttcgacgc gcgtgcggtc agtgcccaga gagaaggcgt cggatgcggc
      901 aacccggaac tcgaccccgg gccccacctg caggtttccc gccaggccga cgatcagatc
      961 ggcttcgagt aaaacaggcg aatcgctggc agtcagagtc accagatcat tgatataccc
     1021 gggcatgaag acgcggcgcg agtagacgcg cggtgagacc gcagactcga gtgaccaggc
     1081 ttcgacggca tattcgtaga agcggccgcc tctggcgccg acgtcggcat acgtcctggt
     1141 gttgcggtcg agaagatcag tggtcagcag tgtccagtcg gtgtcctttt cgcggcgcca
     1201 gacgcgatat ccgacgacgg cggtcgaggt cgagagatcc cattccaggt cgacgacgcc
     1261 aaaggcagcc agaggttgaa tgcgcacatc aaccggagcc gcgggaatcg gtccctgggt
     1321 cgtcttaaac ggcatatcgt acgaaaacca ggcgttgttg ttgtcgtcaa acagcttggc
     1381 acgcacgtga tagacggtgt ccggcgccag accggtgatt gtgtaccggt gatcggtcac
     1441 ggcttcccgc tcggtatagg tgtttccgta gctctgcgtc agaccccact cgagaatggt
     1501 cgtcgccggc ttgtaggtct tccaggaaat gtccgcggtg gtcgaagccg ttcgctcgat
     1561 caccagcgcg gaaagctgct gcgaagaagt cgcccagtcc agcttgattt catcatacgc
     1621 cacattggtg ctcggcggaa tctcgaccac tcgtgattca atacgatagg tcgggtgggt
     1681 aatgacaatc gtgtttcggc cagacggcaa ctcggacaaa tagaacgagc cgtcgttgcc
     1741 cgtgtaaacc tgctcaactt cgttgaacag cgagtacacc ttggcgttct cgacgatctc
     1801 gcccttcacg ttgaaaacgc gccccgagag gcttcccgtc tggggttcgc gcaagccgca
     1861 gcccatcagc ccgagaagca gaaaggcgac gaccagaggc gccgcccgga atctgcgtat
     1921 gaccgcacta cggaacatca taggctccga tgtcctcgaa tccgtatcct tcccgatcga
     1981 ggcgactgcc gggagccggc cgatacgtga atgaagcgtt cggatactca acgaacggcg
     2041 tgccgccctc gaagctcggg tcgagatacc ggtcattgtt cacacccggt aaaacagctc
     2101 cacgaatatc aatgtatcga ttcgaactcg tacttggcag atcccagatg tcgttgaagg
     2161 tgatcgagtg tgaagccggc acgtaggcat cagcactgtt gtacaacact ccgatcgcgc
     2221 ccttgcgctg gtcggcgaga atcagattgc tgcgcagagt cgcctgtgcc gaatcgcgaa
     2281 tctcgatgcc gatctgcgcc tgaatggtgt tgcgacgaat ctcatcccgg ccgcgcacga
     2341 cgattcccgt gcccgtgccg ccggggccaa tgatgttgcg accgatgaaa ctgctggtcg
     2401 tcgcattccc gatatccatt cccgtgacgt tccagaactg aaccttgttc ccgaccaccg
     2461 tggcccgttt gttgtcgcgg gcgacgatgc cgtagaaaca gcgaacggcg gtgcacgtcg
     2521 caatgctgat ttcctgggtc gtattgctgg cgatgagaaa cccggacgcg caggtatcag
     2581 cgatcgagtt ggcgatcgag accggagccc gggcggcaat acagcgaaca ccggccgcgc
     2641 tgcagttggc aaaacgcgaa ttactgatcg gcggtgctcc ggtaatgccg tgaacgccat
     2701 attgggcgaa tgccaggttc aggtaatcaa tgcgcgagac gtacaggttc gctgtcgtat
     2761 agaacccgat ccccacccag tctccggcac gaggagccgc gctcagcgac gtcatcgaaa
     2821 ccggcgccgc ggcgtttcct tcgaccacaa gcgcgccctg caccttgatt tcgacctttt
     2881 tgtccgagcc gctggtgagg tcccactggt cgctggcggt gacgcctacc tgtacgcccg
     2941 gcagaatggt cagagtggaa tcctgtcgaa tggtcagatc gcccgtgagc gtcagaggcc
     3001 cgaactcggg tcgccattcg atgtttccgc gcgtgatacc cggcaacagg aaggacacga
     3061 tctgactcgg cgggctttct tcattcgtgt tggccacgcg tgtgacccgg tagaacacca
     3121 cctgccccgg cagcaccaag gtgtcggtat agcgagtgct cagaagaaga gtcgaatcaa
     3181 cggattcaaa cgggccttgc ggagtcatcg cccggtacag tcggtagccg gccagatcgg
     3241 ttccggtatc cgcgctccag gtaacgatga tggcatccga gtagggatct ttctcgacct
     3301 tgagattcaa tggcgggtcg ccacgctgag tgtaggcggt cgtgaaactt tttccctctg
     3361 aatagatgat atgcccgagg gtgtcagaag caacggcctg aatgtagtag gtactggccg
     3421 gggtgagatt cgtgagcgtg aaactgtgcg ccgtggtgct ggtcgcgatc atctcggttt
     3481 tgctctcata caacgtgttc gggccatact gcacataggt gcgcgccagc cctcgagtat
     3541 agaaacgaat cgtcgcagct gatgacgtca catcgagaat cgagaggcca tagagaaagc
     3601 cctcgtcttc catggtcaga atgacgtttt cggtgatcac tccggcaaga acctcgacct
     3661 gtcgggaagc ggcagcaaac ccttcgcgtt cggcgagcac ggtgacgatg gccggggtga
     3721 gaccatcgag gccccaggtt ccgtctgagg cggtgatggt tgaagcactg gtgacggtga
     3781 tacgcactcc gctgagccga gtgccgttca cgtcgacgac gatgccgcgc aacccgcccg
     3841 gggtctgctg tttggtttca agacacccga gcatggcccc gacgatgacg acgagacaca
     3901 agacaaatac gccacgatgt tttatcatgt ctgcactaag tcttcggaag aaaccgccca
     3961 atcgtttaga gggcaacgga gacaacacag aacgcaccga aggaaaagga agggagcagc
     4021 acatgaacat ctctcaacgc gcagccagca tgcaggcatc accgattcgc aaattgattc
     4081 cgctggccaa tgccgcccgc aagcgcggag tgacggttta ccctctcaat atcgggcagc
     4141 ccgacattca caccccggaa ccggttcgcg aagccattcg cggctttgat cagaaggtcc
     4201 tggcctacag cccgtcaccc ggtgaagatt atctgctcga ggccttttcc cagtattacc
     4261 gcaaaaacaa cattccgctc gacccggcgg aaatcattgt caccaccggc ggcagcgaag
     4321 cgatgtattt cgcgtttctc gcgatctgtg acccgggcga tgaagtgctc gtgttcgagc
     4381 cgttctacac caactacaac ggcatggcac accagacggg ggtgacgctg aaagccctgc
     4441 gcaccgtcgc cgaggacggc ttccagctgc ccccgatcga ggtgatcaag aaggcgatca
     4501 ctccgcgcac ccgggcgatt ctgatctgca accccaacaa cccgaccggt acggtttaca
     4561 cccgccagac cctcgaagcg ttggcgcatg ttgccaagga acacaaggtg tatctgctcg
     4621 ccgacgaagt ctatcgtgag ttcgcctacg atgggctcgt gcacacttcg attctgcatc
     4681 tgcccgaaac ctacgacaat gccattctga tcgacagtat ttcgaagcgg tactcggcct
     4741 gcggggcccg catcggagtc atcgcgagca agaacaagga cgtcatgagc gcgtgcctga
     4801 agctggccca ggctcgtctc agttcgccga cggtcgagca atgggcggcc cgagccggtc
     4861 tgctgatgga tccctcgtat ttccgtccga ttctggaaga gtaccagcgt cgtcgtgacg
     4921 cggtcatgga aggctttgcc aagattccag gctgcgtctg caagactccg aagggcgcgt
     4981 tctacgtcat cgccaagctg ccgattcaga acgccgaagc cttcgcgagc tggctgttga
     5041 ccgaccacag ctacaataat gaaacactgc tggtcgcccc ggccgctgga ttctacgtca
     5101 cagcgggcct tggcaaagac gagattcgca tcagctactg cctcgaggtc gacaagctga
     5161 aacgcgccat gaccaccctg gccgaagggg tgaagcgttt tcaggaactc gaaaaaacca
     5221 agcactgagg aattcgagtc cgggagccga gaaccggctt ccgggctctt gcctgaccaa
     5281 ttgcagatcg gactatcgcg tttcaaacgc cacttgagcc agggccacga gggagatttg
     5341 acacgatgag tgatacacca gcacctttgc agattcgcct gcaacccgcc ggaacctggc
     5401 cctggcaagt tcccgcccga gcgacgaccg gcgcatcggg ctacgatctc tgcgccagcg
     5461 tcaagggcga gatcggcccg ggaaaagttg tcgccgtgcc gaccggcatc aaaatcgaga
     5521 tacccgcggg gtacgaaggt caggtgcggc cacgttcggg gctcgcggcg aagcacgggg
     5581 tcacggtgct gaacgccccg ggcaccatcg acagcgatta tcgcggtgag gtgaaggtca
     5641 ttctcatcaa ccacggtgaa gagccgttcc gctacgaacc cggcatgcgc atcgcccagc
     5701 tggtgttcgc aaaagtccag gaggcggagt ttacggttgt tgatcaaatc gttgactccg
     5761 cacgcgggga aggtggtttc ggatcgacgg gaaccttctg acgaatgccc cttgcccgcg
     5821 gcggtttttt ctggtacgaa agtctgcgtt actgattcag gacgaaggag tgtgcaatga
     5881 gtaaacgccg ggtgtgtgtc attggctgtc tggggaagat ggggcaggtc acctgccgcg
     5941 gactgctctc caccgccgac tacgaagtgg ctgcagcggt cgatgtctcg cacggcggcg
     6001 aagatctggg cgagttgatt cacatgggtc acctcggggt gacgattgcc gacaacctgc
     6061 aatccgtact ggcgcaggaa aagatcgacg tcgccatcga tttcaccaat cccgattccg
     6121 tggtctccaa tgtcgccatg tgcctgcagg ctcgcgtgcc ggtgctggtc ggtacgaccg
     6181 gctggtcgga agaagatctc gagaaggtgc gcctgctgtc gaacaagatg aagacgccgg
     6241 ttctggtcgc ccccaacttt gcgatcggcg ccgtgctgat gatggagttc gcgaaaaagg
     6301 ccgccaagta catgccgcat gtcgagatca tcgagatgca caacccgaac aaactcgaca
     6361 aaccatcggg gaccgctcgt ctcacccgtg aactgatgct gaagtcagtg ggtcgcaccg
     6421 atcgcgatcc ggaagagttg attccgattc attcggtgcg cctgcccggc atggtcgcgc
     6481 accaggaagt catcttcggc gaagtcgggc agttgctgaa gatcagacat gattcgatgc
     6541 agcgcgaatc cttcgtgccc ggcattctgc tcggcctgaa acagctgttt cataccgagg
     6601 gtctgaaaat cggcctggag ctgtaaccag agtttcaccc gttcagattc tttttgtctg
     6661 agcatctgac ggattcatga ctcggacaat cgcgggcgag tattgtcgag atcgaacagg
     6721 gagcaacgca tgaaagttct gcacttcttc gtcaaggatc tcggcggcga gtttccgtct
     6781 gatcgttttc ccatcgtggc gaccagcttc ggcgaggtcg tgcccgaaga gatcgccaac
     6841 gtgtattccg ggtatttcac cgcttcgggg ctggcgccgg tgcgcattcg cctgcgcatg
     6901 gaagacatct cggtcggcag cacgaaagcc gttctgcggg tcgatctcaa gttgttcgct
     6961 tttggggtgg tcattctcga gtatctgatc gagccgacca gcccggtcgc cgacccgaac
     7021 cagctgtttc acgcgcgttc cgtcgaacgg gcctccggcg agaccgtcac cctcaatgcc
     7081 ctctggcagg ccgacatggc cttgctgaga gaccgcctgt atcgcgacat caagcgtgag
     7141 tatgacgacc cggcgtttac cgaccatttt cacctgttca tcgatcacga gaagcgtccg
     7201 gaagaggaag ccatggcgat tctgctgccg ggcgaagagt cctattcgac tccgatcaag
     7261 agacgcatcg tgcaacgcat tcagctctgg gaagaaagtg agtcatgcta catcatgggc
     7321 agtcgcgggt atctcggcac cgcgctgaac ccgttcgaca tcgtcaactt catgcaactg
     7381 gcccgtatgc agctctatga gctgaaagtc tacgatcata ttctggacaa aagcatcgaa
     7441 gacacgtatg cgttcctcga caagctgccg cagaccaact ccgtgcttcc cttttcgtgg
     7501 ttgcatcgcg agtttcagga gcaggtacaa catgcgacca agctgctcgc cgtccgtatg
     7561 gatctcgtcg acctggtcaa ggacatcacc aacacgacca agatcaccga cgacccctac
     7621 ttcgagctgg tctatcgcaa cctaatggat gcgttccgcg tgcgcgagtg gttcgactcg
     7681 gtcaaggaca aaatcgaaga agtcgaagcc agccagaaga tgattctcga ccgccttgac
     7741 atggtgcgct cgaccacgct cgaaatgacc attatcgctc tcattgccgt cgagatcatc
     7801 gttcccttcg gccactggat catgtccttc gtccgctagc gaacggtcgt tttctcgttc
     7861 agaactgagg agcttcccct cgacatcggc gctgattccg gtgtttcgcg agccacccgg
     7921 cagatgatcg ccccagtcga tcggcaacac gctgatctac tgcgggtttc aagcacaaga
     7981 aaaaaagttc ttcatcgcgt tgccacgaac gccgatagat atcgtgaaat tcagattttt
     8041 taccggaggc aaccgaccat gatcattccc gataccccct tgtataacgc caagtccgat
     8101 gcggaattcc gcctgcgcat gattcagcac atcaaggacg cctacgccga ggtgctcggc
     8161 aaggatgtca aactgctcga tctgcccaac ggactcgacc tggtgcaggc caagattgcc
     8221 ggcatgctct acgactcctc gcgtggcgaa atcgatctgc gccgcagtcc ggccgcgcag
     8281 cgtgacgagc ggacgcagta cgccggcacc ggcactgccg tcggcattga tctgcacgct
     8341 gacgccgctg ttggtgtcga ccagctgcac gtagccaccg ctccgggcca cggcccggga
     8401 gatgccgagt gacgccagca gcgcgctcag atccaggcgg tcggtgcccg gcacgaagtc
     8461 ggtgatggtg tcgatgccgt cgcgcagac
//
LOCUS       DAORHD010000028         8369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8048_length_8369_cov_3.656242, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000028 DAORHD010000000
VERSION     DAORHD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8048_length_8369_cov_3.656242"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKO06_01235"
     CDS             complement(<1..361)
                     /locus_tag="PKO06_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HNV68290.1"
                     /translation="MPLRRVVVVANPRAGLRVGGALNEAIELLKRHVETQVEYTRHAG
                     HAEQLARDLGADPDTLVAVCGGDGTLNEALNGIPSRGVLGLLPAGTANVVARELGIPL
                     NLREAAKVVAGRKVASGV"
     gene            complement(373..1257)
                     /locus_tag="PKO06_01240"
     CDS             complement(373..1257)
                     /locus_tag="PKO06_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV68291.1"
                     /translation="MQIGPFTVDVLSEGTMMLDGGAVFGGLPRTAWESRLPPDPQHRV
                     PLEIRQVLVRRPGVNLLIDTGFGEKVSPQIRSLYGLGKLRSWQERLGPLGLAPEDITH
                     VVFTHLHLEHAGGATRYAESGEELVPMFPRARHYVQESEWKDACVPNERSRAAYRFED
                     FLPLQHESQVQMVAGDQEIIEGVRVQLTGGHTRGHQIVIVGTREQSLFVTGDLIPTRH
                     HVSPAWLAPGDQSPQDLLESRKSLLRRLLQMPEHVLLTAHDRGTTPVRVSGSLESLQI
                     AEPGQASTPCGVELPLAV"
     gene            1345..2118
                     /locus_tag="PKO06_01245"
     CDS             1345..2118
                     /locus_tag="PKO06_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68292.1"
                     /translation="MADTFDSSNVTPTATAVMRTLGPLIREHALWEPGERLAIGLSGG
                     KDSLTLTWLLSEATRFHLPECHIVALHVPPPPWCSCAVPVEELRRLAASLPRVELQIL
                     ASDEPLPESCDRCARERRRRLFLACREREIHTLAFAHHQDDLVQTLLMNILFHGEASA
                     TMQPVRSFGDGALRLIRPLLSTPEKRIIALARRLGYLRSVTCATSGPAESQRTAVRAF
                     LHGLGPRRDVVKANLARLARQFRSAESSTPASEPASPSK"
     gene            complement(2058..2810)
                     /locus_tag="PKO06_01250"
     CDS             complement(2058..2810)
                     /locus_tag="PKO06_01250"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HNV68293.1"
                     /translation="MSGHRGPWPGDEQCFEPEQVKLLQRAVVHLSWLLSREYSFKSSL
                     QLVGDRFQLVARQRLAVGRSACSDEARHRRRARCRPMTAVGLGPLLIDGFNLLITIES
                     ALAGGVLFAGRDGAIRDLASVHGSWRRVAETERAMQIIAAALATAGGPECIWFLDAPV
                     SNSGRLCGILKQMAQERGLPWSVELTMNPDQKLAQASGLVVSSDALILDRAEFWVNVT
                     ETVLTTFDQVQLIHFDGEAGSDAGVDDSAERN"
     gene            complement(2807..4783)
                     /locus_tag="PKO06_01255"
     CDS             complement(2807..4783)
                     /locus_tag="PKO06_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV68294.1"
                     /translation="MSLQVKDLAKSFGTRQLFASVTFAVNPGEKVALVGGNGSGKTTL
                     MRIMLGEEHPDAGRLTKPAEARVGYLPQEVFLVEGADGSTDRTITLWQLAAQAFDRLE
                     GIKTEIERLENQMGQGDIDHALFEKHERLVAQFERAGGYTWQARLTRILKGLGFSESR
                     FHEPFAHFSGGWRMRGYFARLLASDPDYLLLDEPTNYLDIGSIAFVEDYLKSFAGGIL
                     VVSHDRYFLDNLATSVVALVPEGARVFRGNYSGFLEARLAWQAEADAAIERQTKERQR
                     IERFIERFRYKATKAAQVQSRIKMLGKMERIERPIDSRVLDFSFPKCQASGEMIMQVK
                     NVNRSFGDLQVLRQVDFSLYRGDRLCIVGENGVGKTTLMRILARQDTGFHGDLSWGYR
                     VYPAYFAQDEEISFEQEETISQRMMRECPIEMVPQLRGLLGAFLFSGDDIDKPVRVLS
                     GGEKSRLGIARLMMRPCNLLMMDEPTNHLDISSREALLEALDEFPGTLIIVSHDRFFL
                     DELATRVLVLDGGRATVYEGNYSQYLWRKQQAAKEEAVQSAGTHDRGSTTVAETNGGS
                     DSGADAWKARRRQNNQRQKLEKENQQIETQISGLEEGIRDIESRLASPRAGTTHEELA
                     QWARDLERSQRELDAAMRRWEELQTQLVEPEQMA"
     gene            complement(4786..5628)
                     /locus_tag="PKO06_01260"
     CDS             complement(4786..5628)
                     /locus_tag="PKO06_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68295.1"
                     /translation="MKNLSSQVTVVPVVSPDEAVARARGGLMWLLITLFLAWLSDSGL
                     GVTDYLRLLQEASREGGHSGAFMSIVLTFFALFGAALGQILGEELAAQLWAREQRLLQ
                     QQKSSPVAGVLHQNLLADPFEWMVSIHGWLNQFWRFLPSGAAQWFDAGGLFERLANLA
                     RRGLCFFAGLSGTFFMAAFMQMVGMEEWMSGSEEHRWQLVAIVLLIGNLFALALTSLP
                     PISLVLVMGLIFVYYGIVGVLAGELVVLKLLVMLQGVFLLLVAIRSIRSRMIPETTGT
                     KAEH"
     gene            complement(5775..6908)
                     /locus_tag="PKO06_01265"
     CDS             complement(5775..6908)
                     /locus_tag="PKO06_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68296.1"
                     /translation="MLPSNVRIIQDDAGVLRVSTSSPAMCMIGLLLLTVAISFVAALG
                     IDPLMQRPWWVIPLIAVCLLGLIFFVGGRILEVSRTTETIRYGWGWRSCAWTFRTGTL
                     RQVKGMELGSFVSLVRRPIEPGADLSKTVPDMYQLSLRWGEEQVKLFDSPNLDALVTL
                     ASKIARFAGWSMLEIVPESVLVEGAVSPQSLLREGSTAGWVPMRMPQERTFVCTQESD
                     GMKIEIEPFRPVWIWITRLILIVLGIGPTVYWLINFSGEIDWIILLLFQSFCWIQAIP
                     LLREQNRFGRFLVVRRDQLQVTDLLTLWGLRTRVIPAAEIVAIKIGHPNRQLDPIGLR
                     DNQQELIIDDGREVLTIGPGFPWLKLAWLRRQILATFFPAPET"
     gene            complement(7064..>8369)
                     /locus_tag="PKO06_01270"
     CDS             complement(7064..>8369)
                     /locus_tag="PKO06_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV68297.1"
                     /translation="LKMNIPAQEKKDETAALHKLQETAAGYARTLGQMIENQLLVEGL
                     SNACGAQAEQCEAVAAALAGRMESVAEAAKTEGSATTTALILIIVLAAAIFAVGMVRA
                     ITLPLRQFIEQLTNGATQLAAASTQVSTASQSLAAGASEQAASLEETSSALEEMSSMT
                     RQNADNAEQANQTARQADTMATTGVTQMETMVATIDRIKTSAGETARILKTIDEIAFQ
                     TNLLALNAAVEAARAGEAGAGFAVVAEEVRNLAMRSAEAARNTAGLIEGAQKNADEGV
                     NVTGEVAKSLKSIQEGAAKVASLINEITTATKEQSTGLGQISTAVSEMDKVVQQNAAN
                     SEESASAAEQLSAQALEVKNIVMELTVMVEGATARSAQASEFDHPQGPPASRVITHPK
                     GRTAPVVKHAPAVATAGKKPATSRKPNPAKVIPLDENDLKEF"
BASE COUNT         1713 a         2746 c         2287 g         1623 t
ORIGIN      
        1 tgacgccaga cgcgaccttg cggcccgcga cgaccttggc ggcttcacgc agattcaagg
       61 ggataccgag ttcgcgggcc accacattcg cggttcctgc cggcagtagt cccagcacgc
      121 cccgagacgg aatgccattc agagcttcgt tcagagttcc gtctccgcca cagacggcaa
      181 cgagggtatc cggatccgct cccaggtcgc gcgccagctg ttccgcatgc ccagcgtgtc
      241 gggtgtactc gacctgcgtc tcgacatgac gtttcagtaa ctcgatggct tcgttcaagg
      301 cgccaccaac tcgcaaaccc gctcgcggat tcgcaaccac gacaacacga cgaaggggca
      361 tcgggacctc gattagacgg ctaaaggcag ctcgacgcca cacggggtcg acgcctgccc
      421 cggctcagcg atctgcagtg actcaaggct gccggaaact cgtaccgggg tcgtaccacg
      481 atcatgtgcc gtgagaagca catgttcagg catctgcagc agacgtcgca acaggctctt
      541 ccggctttcc agaagatcct gtggggactg gtcgcccggt gccagccacg ccggtgacac
      601 atgatgacgg gttggtatca gatcccccgt cacgaacaga gactgttcac gcgtaccaac
      661 aatcacgatc tgatgtcccc gcgtatgccc gccggtcaac tgcacgcgca ccccttcgat
      721 gatctcctga tcaccggcaa ccatctgtac ctggctctca tgttgcagcg ggagaaagtc
      781 ttcaaagcga taggcagcgc gggaacgctc gttgggcaca caggcatcct tccactccga
      841 ttcctgcacg taatgccggg cacgtggaaa catcgggacc agttcttccc cgctctcggc
      901 gtagcgtgtg gctccgccgg cgtgttccag atgcagatgc gtgaacacca catgcgtgat
      961 gtcttccggg gcaagaccga gcggccccag tctttcctgc cacgaacgca gcttgccgag
     1021 gccgtacaac gatcgaatct gcgggctgac tttttcgccg aacccggtat cgatcagcag
     1081 gttgaccccc gggcgacgca ccagcacctg cctgatctcg agcggcaccc gatgctgagg
     1141 gtcgggagga agccgggatt cccaggcggt gcgaggaagc ccgccaaaga ccgcgccgcc
     1201 atcgagcatc atggttcctt ccgacaacac atcgacagta aacggcccga tctgcatgac
     1261 ttctctcctc gctgcgccag agtgtgtggc ctctattatg ccataaccct gcccgtttct
     1321 ctatcgatct ggtagcatgt cggcatggcc gacaccttcg acagctcaaa cgtcactcct
     1381 actgccaccg ctgttatgcg gaccctcggc ccgctgattc gcgagcacgc actctgggag
     1441 cccggcgaac gtctggccat tggcctgtca ggcggcaaag acagcctgac cctcacctgg
     1501 ctgctgagtg aggccacgcg gttccatctg ccggaatgcc acatcgtggc cttgcatgtt
     1561 cccccaccac cctggtgctc ctgcgcggtg ccagtcgagg agttgcgccg acttgcagca
     1621 tctctgcccc gagtcgagct gcagattctg gcaagtgatg aaccgctgcc cgagtcgtgc
     1681 gaccgctgcg cccgggaacg acggcgccgg ctgtttctcg cgtgtcgcga gcgcgagatt
     1741 cacacgctgg cttttgccca tcatcaggac gacctggtac agactctgct catgaacatt
     1801 ctgtttcatg gtgaagccag cgcgacgatg caaccggtcc ggtctttcgg ggacggagcc
     1861 ctgcgcctga tccggccgtt gttgtcaacg ccggagaaac ggatcattgc cctggccaga
     1921 cgcctggggt acctgcgatc ggtgacctgt gcgaccagtg gaccggccga gtctcagcgc
     1981 acggctgtgc gcgcttttct gcacggactc ggcccacgac gcgacgtggt caaggccaat
     2041 ctggcccgcc tggcccgtca gttccgttcg gccgagtcgt cgacgccggc atccgagccg
     2101 gcctctccgt caaaatgaat cagttgcacc tgatcaaacg ttgtcagcac cgtttcggtg
     2161 acattcaccc agaactccgc tcggtccaga atcagcgcat ccgacgagac gaccaaccct
     2221 gacgcttgcg ccagcttctg atccggattc atcgttaact ccaccgacca gggcagacct
     2281 cgctcctggg ccatctgttt caagatcccg cacagtcggc cgctgttcga gacaggcgca
     2341 tccagaaacc agatacactc tggtccaccg gcggtcgcca gagcagctgc gatgatctgc
     2401 atagcccgct cggtttcggc aacccgtctc caggaaccgt gaacactggc caggtcacga
     2461 attgcgccat cacgtccggc gaagagcacc ccacccgcaa gcgcgctctc aatcgtgatg
     2521 aggagattaa aaccatcgat cagcagaggt ccgagtccca ccgctgtcat cggtcggcac
     2581 cgagcccgac ggcgatgtcg cgcctcgtcg gaacacgccg atcgcccgac cgccagtcgt
     2641 tggcgggcaa cgagctgaaa ccggtctccg accaactgca atgacgattt gaaactgtat
     2701 tcgcgtgaaa gcaaccagga caaatgcaca accgcccgct gcagcagctt cacctgctcg
     2761 ggttcgaagc actgttcgtc acccggccac gggccgcgat gtccgctcac gccatctgct
     2821 ctggctcgac aagctgcgtc tgcagctcct cccatcgtcg catggcggca tcgagctcac
     2881 gttgggaacg ttcgagatcg cgagcccatt gtgccagttc ctcgtgagtg gttccggctc
     2941 ggggcgaggc aagacgagac tcgatatcac gaatgccttc ctcgagcccc gagatctggg
     3001 tctcgatctg ctgattttcc ttctcgagtt tctgtcgctg gttgttctgc cgccgccggg
     3061 ctttccaggc atccgccccg ctgtcggaac caccgttcgt ctcggccaca gtcgtcgaac
     3121 ctcggtcatg cgtaccagca gactgaacgg cttcttcttt ggccgcctgc tgtttgcgcc
     3181 agagatattg actgtaattc ccttcataga ccgtcgcgcg accgccgtcg agcaccagaa
     3241 cccgggtcgc cagttcatcg aggaaaaaac gatcgtgcga gacgatgatc agtgtaccag
     3301 ggaactcgtc gagcgcttcg agcagagctt ctcgactgct gatatcgaga tgattcgtcg
     3361 gctcgtccat catcagcaga ttacagggtc gcatcatcag ccgggcgatg cccaggcgac
     3421 tcttctctcc gcccgacaga acacgcaccg gcttgtcgat atcgtcaccc gagaagagaa
     3481 acgcgcccag caatccccgc aactgcggca ccatctcgat cgggcactcg cgcatcatgc
     3541 gctggctgat cgtctcttcc tgctcgaaac tgatctcttc atcctgcgcg aagtacgccg
     3601 gatacacgcg atacccccag ctcagatcac cgtggaaacc ggtatcctgt cgggccagaa
     3661 tgcgcatcag cgtggtcttg ccgaccccgt tctcacccac gatgcagagg cggtcgccgc
     3721 gatacagcga aaagtcgacc tggcgcagca cctgcagatc gccgaaactg cggttgacgt
     3781 tcttgacctg catgatcatc tcgccactgg cctgacactt cggaaacgaa aaatcgagca
     3841 cgcgactgtc gatcggacgt tcgattctct ccattttgcc gagcatcttg atgcgactct
     3901 gcacctgcgc cgccttggtt gccttgtacc ggaagcgctc gatgaaacgt tcgatgcgct
     3961 gccgttcttt ggtctgtcgt tcgatagcgg cgtcggcctc ggcctgccag gccagacgag
     4021 cttcaaggaa acccgaatag ttgccgcgaa acacgcgcgc cccttctgga accagtgcca
     4081 cgaccgaggt tgccaggtta tccagaaagt accggtcgtg cgaaacgacg agaatgcctc
     4141 cggcaaagct cttgaggtag tcttcgacga acgcgatcga accaatgtcg agatagttcg
     4201 tcggctcgtc cagcagcagg tagtccgggt cactggccag cagccgggca aaatatccac
     4261 gcatgcgcca gccgcccgag aagtgcgcga acggttcatg aaacctggac tcggaaaaac
     4321 ccagaccctt caggatacgc gtcagacgtg cctgccaagt gtatccgcct gcccgttcga
     4381 actgggccac gaggcgttcg tgcttctcga acagggcgtg atcgatatcc ccctgaccca
     4441 tctggttttc cagtcgctcg atctcggtct tgatcccttc caaccgatca aaagcctggg
     4501 ccgccagttg ccagagggtg atcgtccggt cggtgctgcc atcagccccc tcgaccagaa
     4561 acacttcctg cggcagatac ccgactcgag cctcagccgg cttggtcaac cgccctgcat
     4621 cgggatgctc ctcacccagc atgatgcgca tcagggtagt cttgccagac ccgtttccac
     4681 ccacgagcgc gaccttttcg ccggggttca cggcaaacgt cacgctcgca aacaactgcc
     4741 gggtaccaaa ggatttcgcg agatctttca cctgcagtga catggtcagt gttccgcttt
     4801 cgtcccagtt gtttccggta tcattcggct tctgatgctg cgaatggcca caagaagcag
     4861 aaacaccccc tgcagcatga ccagcagctt cagcacgacc agttcgccgg cgagcacccc
     4921 gacgataccg tagtagacga agatcaagcc catgaccaga acgagactaa tcggaggcag
     4981 actcgtcaga gccagagcaa acaggttgcc gatcaagagg acgatcgcca caagctgcca
     5041 gcggtgctcc tcgcttcccg acatccattc ttccatgcca accatctgca tgaacgctgc
     5101 catgaagaag gtgccgctca aaccagcgaa gaaacaaaga ccacgacggg ccagattggc
     5161 caaacgttcg aacaacccac ccgcgtcaaa ccactgagcc gctccagatg ggagaaatct
     5221 ccagaactga ttgagccagc catgaatcga gaccatccac tcgaatgggt ccgccagcag
     5281 gttctgatgc aagacaccag ccaccggcga cgacttctgc tgttgcagca ggcgttgttc
     5341 gcgggcccag agctgcgccg caagctcttc tccgagaatc tgcccgagag ctgctccgaa
     5401 cagggcaaaa aaggtcaaga cgatggacat gaacgcgcca ctgtgtccgc cttcgcgact
     5461 ggcttcctgc aacagccgca ggtagtccgt tactccgagc cccgagtcgc tcagccaggc
     5521 gagaaaaagt gtgatcaaca gccacatcag accaccgcgc gcacgcgcga cggcttcatc
     5581 ggggctcacg accggcacga ccgtaacttg agacgaaagg ttcttcatgg gttcaccggc
     5641 cggcagtata caccagaatc tccgttcatc ggcaccactg ggcgcaaatt cgatggcgac
     5701 cgagttgctg aagcactgcg cgtctcacgc agactcgtac cgccgtcggc ggcaacccgg
     5761 tgactcgcaa ccaatcaagt ttcgggtgca gggaagaagg ttgccagaat ctggcgacgc
     5821 aaccacgcca gcttcaacca cgggaatccc ggaccgatcg tcagcacttc gcggccatca
     5881 tcgatgatca gctcctgctg gttgtctcgg agtccgatag gatcaagctg ccgattcggg
     5941 tgaccgatct tgatcgcaac aatctctgca gccgggatca cccgcgtgcg caggccccag
     6001 agagtcagca gatcagtcac ctgcagctga tctcgccgca ccaccagaaa ccgtccaaaa
     6061 cgattctgtt cgcgcaacag cggaatggcc tgaatccaac agaaactctg aaacaacagc
     6121 aaaatgatcc agtcaatctc tcccgagaaa ttgatcaacc agtacacggt cggcccaatg
     6181 cccagtacga tgaggatcag acgggtgatc cagatccata ccggccgaaa cggctcgatc
     6241 tcgattttca tcccgtcaga ctcctgcgta cagacaaagg tgcgctcctg cggcattcgc
     6301 attggaaccc agcccgccgt gctaccctcg cgcagcagcg actggggcga aaccgcgccc
     6361 tcgaccagca cgctttcagg cacgatctcg agcatcgacc agccagcgaa acgcgcgatc
     6421 ttcgaggcca gcgtaacgag tgcgtccagg ttaggcgaat cgaaaagctt tacctgttct
     6481 tcaccccagc gcagagagag ttgatacata tcaggaacgg tcttggaaag gtcagccccc
     6541 ggttcgatcg gacgtcgcac cagactgaca aatgatccaa gttccatacc tttcacctgg
     6601 cgaagtgtac cggttcgaaa agtccaggca cagcttctcc agccccatcc atatctgatg
     6661 gtctccgttg ttcgtgagac ttccagaatt cggccgccaa caaagaagat gagccccagc
     6721 aagcaaaccg caatcaacgg tatcacccac caggggcgct gcatcagagg gtcgatccca
     6781 agcgctgcca cgaacgagat ggcaacggtg agcaagagaa gtccgatcat gcacatcgcg
     6841 ggactgctgg tagaaacacg aagaacgccg gcatcatcct gaatgatacg cacgttcgat
     6901 ggcaacacgc ggaactccct ttcggccgca ataggctgtc agcggctgat catcaagcta
     6961 ccccgctctg ataccgctgt caatcttcag ctgagttgcc gccgcctcgc gcgtacatac
     7021 tgcggggctt ggcagatctg ccaagccccg cacgtgaaga cgatcagaac tctttcagat
     7081 cattctcgtc gagcggaatc accttggccg ggttcggctt cctgcttgtg gcaggcttct
     7141 ttccagctgt tgcaaccgcc ggcgcgtgct tgacgacggg cgcggttctt ccctttgggt
     7201 gagtgataac tctggatgcg ggagggccct gcgggtgatc gaactcggac gcttgcgccg
     7261 accgtgccgt tgccccctcc accatgaccg tcagttccat cacgatgttc ttgacttcaa
     7321 gcgcctgggc cgagagctgt tcggcggccg aagccgactc ttcagagttg gcggcattct
     7381 gctgaaccac tttatccatt tcagaaaccg cggtgctgat ctgtccgaga ccggtcgatt
     7441 gttctttggt tgcggtcgtg atctcgttga tcagcgacgc gaccttcgcc gcgccttcct
     7501 gaatgctctt gaggctcttg gcgacttcgc ccgtcacgtt gacaccctcg tcggcgttct
     7561 tctgcgcgcc ttcgatgagc cctgccgtgt tccgcgcggc ctctgccgaa cgcatagcca
     7621 gattacgcac ttcttcggcg acgacggcga acccggcacc ggcttcaccg gcgcgagcgg
     7681 cctcgacggc ggcattcaac gccagcaggt tcgtctgaaa cgcaatttca tcgatggtct
     7741 tcaagatgcg agccgtttct ccggccgagg tcttgatgcg atcgatcgtc gcgaccatcg
     7801 tctccatctg cgtcacaccg gttgttgcca tggtatcagc ctgacgagcc gtctgattcg
     7861 cctgttcggc attatcagca ttctggcgcg tcatcgacga catttcctcg agtgccgagg
     7921 aagtctcttc gaggctcgcg gcctgctcgc tggcgcccgc ggccagcgac tgactggccg
     7981 tcgagacttg cgtcgatgcc gcggccagtt gagtagcccc attcgtcaac tgttcgatga
     8041 actgccgcaa cggcagcgtg atggcacgca ccatgccgac ggcaaagatc gccgcggcaa
     8101 gcacgatgat gagaatcagt gccgtggtcg tcgcagagcc ttccgtcttc gccgcctcag
     8161 ccacggactc catgcggccc gccaaagcgg cagccactgc ctcacactgt tccgcctgag
     8221 cgccgcaggc gttcgatagt ccctcgacca gcaactgatt ttcgatcatc tgaccaagcg
     8281 tccgcgcata ccccgcggca gtctcctgca acttgtgcaa ggccgcggtt tcgtccttct
     8341 tttcctgtgc cggaatgttc atcttcagc
//
LOCUS       DAORHD010000029         8316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8118_length_8316_cov_4.355526, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000029 DAORHD010000000
VERSION     DAORHD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8118_length_8316_cov_4.355526"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKO06_01275"
     CDS             <1..939
                     /locus_tag="PKO06_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV68298.1"
                     /translation="AMMLHERVSRSRSRRPEGEPPAAEPTTPQPPKEKITIDQLEAMF
                     VQLGENYAAKVAKEASYLGTFKARVINCSFGSENKSLCQVFKQNMVQEWGWENPSDAE
                     VQQVVNLFVEKALLAKDKALFAQCPEALFCIAAGNSSEDLDEIVTSPNDVPIDNKLVV
                     AATLENKKIADFSCHGVTKVDVAVPGVNIYATYPNGKMGFMSGTSMACPMAARFATQV
                     LQAHDGLTAVEVKKILMGTVDKKDWLADKVRSSGVINPNRAIHAAKLVKSGKSLDTAI
                     AEALTAVMDVQPRRSPYKGPNLKDKQVRDLYFSAIF"
     gene            1023..1970
                     /locus_tag="PKO06_01280"
     CDS             1023..1970
                     /locus_tag="PKO06_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68299.1"
                     /translation="MTLWRTTWLSLLILATLVIVPELQAQELTLFTMPPPRSLNWASP
                     WTLAFDAAVGNRFTFTHIKHKHTFGHVFIELKGPNGERELTGSTTAPDAPTDSDKVTK
                     EGYGLGVFWADLKGALETPASLDPQILDRYKSGRIGFITFKLHPDTYQRLARFLREYR
                     ERGYGNIYNGMNLPREGRGAGCSIFGVVFLELAGLLHPEFVRDWIISVRVPESLIGGP
                     LTGKKISIKNAILSRWAKQDEKHRVLSLYDPDLIFEWILKEWEKGKFDQLHDTSSADR
                     GYLSRKQLVRRQKALGLTYDVSDIAPPTDPIWQGQAAAK"
     gene            2073..3458
                     /locus_tag="PKO06_01285"
     CDS             2073..3458
                     /locus_tag="PKO06_01285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV68300.1"
                     /translation="MASNALRRHNAFLLFVWLVVFACSAFVPGSVLARSHLELSPDSA
                     REVLPAALRLLAHQLRRDGSLALFDFDGTLCSEQYLAPDGRFRSGQSTWHLWGARHLQ
                     EYPRLFLQQRLGFDHDEDARLISDKDDVLEGLTDIESHGYMKFAQIACFEAGMTPGEL
                     HTGLEGFLQEYQPADYVYPVMIDFLEVLLRQGTRVWIVSGSNPHYIHAVLRQLARQRG
                     EASETLLRHGHLIADTYSPYDPAAGTGCRLVGNMARQGRDTRFRAIYDDRFTPASGSA
                     RFIVEREGKWRAFETFIKPRDGAAIGLVAGNSDGDFELIDAAMKANSRLTALLVNPRG
                     SHLNTLPKSRCLTIKQQSRKGGEGMTRVTGTAQNAKLGPLVLTSNGQVWVLGLEEWPP
                     EAVGQEVEVCGTTEERSDLPVFVQPVSGPISQGIPVEPGTDLSQAAKRTCLTLANWHL
                     RSTQPKRGAKP"
     gene            3455..4363
                     /locus_tag="PKO06_01290"
     CDS             3455..4363
                     /locus_tag="PKO06_01290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV68301.1"
                     /translation="MRTLWFTLFVLLSLAAPLAAAEPPDTLYTLPYPSGQEYLVSVAY
                     NDWPTHVGNYAIDWLMDEGTHILAARDGVVARVVNTFTEAGLTPDFLQKNNLLTLKHP
                     DGTYTDYVHLQKGGIKVRVGQSVKAGELIALSGNTGFSSAPHLHFIAYRLGKNGAQES
                     FPVRFVSGMAKPYEIFRDCRYLAPGPDCQPTVDPFEAAFPGELAKLKFKLADMARAQV
                     SPRAGALAVRDHLKAHGAEYKQKYRVIYAAAQEGDSESLRLLTAFASSMDLRTDPNID
                     RLFQDSAAAPIAEEALELWNEIQVIE"
     gene            4504..5682
                     /locus_tag="PKO06_01295"
     CDS             4504..5682
                     /locus_tag="PKO06_01295"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HNV68302.1"
                     /translation="MSNTFRQRKDDMAKKKITVLGAGMVGAVMALDLAAEEQFQVTIA
                     DRDPEALSRVRAKARLETIEADLSRVEKIRELIKDADLVVGAVPGFLGFQTLKTVIEC
                     KKNFCDIAFMPEDFLQLDALAKQNGVTAIVDCGVAPGMSNLILGKYGSEYQTFEEALI
                     MVGGLPRVRTWPYEYKAPFSPIDVIEEYLRPARYVRDGKIVTMPALSEPELVNLPGVG
                     TLEAFNTDGMRSLLFTIKCPNMKEKTLRYPGHIEIMRILRETGFLSYDPIEVKGSKIA
                     PIDLTTKLLFPIWKAKPGEEEFTVMRVQTTGIRDGKRTRNTYDLLDFTDAKTGFSSMA
                     RTTGFPNVIMARQVLSGAFTRTGVFPPECFGGDKKVFQALTDGLKQRGVVFNHREEVL
                     "
     gene            5790..>6830
                     /locus_tag="PKO06_01300"
     CDS             5790..>6830
                     /locus_tag="PKO06_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNV68303.1"
                     /translation="MSHRISPRRFVGSALFGILIMTACLITPTVTAADRRLDTSIPSV
                     STSVQGNGARVALLIGNTNYDEAPLSNPVNDVRAFAGALKKIGFEVVVRENATHREMD
                     EAIVEFWNRLKGKDVGLFYYSGHGMQVNGENYLVPIKAVVRTEIDIRHGCYAAQKILD
                     YMQDAKPGLSLIILDACRNNPFRRSIRSVARGLAPMTTGKQVLLAYATDPNNVAQDGE
                     PQGNSPYMKHMLRHLEKPGLPIEKFFKEVRKGVLLDTNGEQTPWESGNLTDDFAFVTG
                     TDSTSDKGSAATTPANVDPKQKASPRELWTAEAEKGNVDAMYVVAYNYDSGSMDFPVD
                     HVAATDWYKRAAE"
     assembly_gap    6831..6840
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6841..7954
                     /locus_tag="PKO06_01305"
     CDS             <6841..7954
                     /locus_tag="PKO06_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68304.1"
                     /translation="AEAGSAAAMKAMADRFEDGIGCEQNPKTAHAWLEKAAAAGDTAA
                     ATKVSERNAQLTNLRERAAKGNGFARYELWRLENHIPEDVSRQEQLLLPAAEKGDQNS
                     ADLLCLLYVRELEVGTPEARRTWLNKFAAKGDANVKFLIAKQFQTGIGLPRDFERAAE
                     LYRQSRDLGHPNAARELQELYDAGQADAQEDSGLAQTLDGLKQKAESGDAASQLKMAI
                     KLAKGEGTAIDMPAAITWIRKAAAQNYPRAMTSLGQLHLGGQEGVKEDPTEGVRLLKQ
                     AAEAGDAEAVVTLMKWHQSKKPGADGDIEALAWAYVLIKRSETLGDIAWAYRDAGNFH
                     VEKLTGSLSTGEIQKARKRQNELLKTISAFKRFDDN"
     gene            complement(7951..>8316)
                     /locus_tag="PKO06_01310"
     CDS             complement(7951..>8316)
                     /locus_tag="PKO06_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68305.1"
                     /translation="VQPWSRIPRWLSGRLTPARVFSVFSPIEETAPYLASVRQLGADV
                     VDVELASLAQFLDTRLGRQTWCKALLIVSDLPGRGSTLDALSERELELEPAFIEMTDL
                     LIADLDLDRPSDHTVPLNR"
BASE COUNT         1810 a         2502 c         2406 g         1588 t
ORIGIN      
        1 gccatgatgc tgcatgagcg cgtctcccgc tctcgctcac gccgccccga aggcgaaccc
       61 ccggcagctg aaccgacgac cccacagccc ccgaaagaga agatcaccat cgatcagctc
      121 gaagccatgt tcgtacagct gggcgaaaac tacgcggcca aggtcgcgaa agaagcgtcc
      181 tacctcggaa cgttcaaagc ccgcgtcatc aactgttctt tcggcagcga aaacaagtca
      241 ctctgccagg ttttcaagca gaacatggtg caggagtggg gctgggaaaa cccgagcgac
      301 gccgaagtgc agcaggtggt gaatctgttc gtcgaaaaag cgttgctggc gaaagacaaa
      361 gcgctcttcg cgcaatgccc cgaagccctc ttctgcatcg ccgccggcaa ttcctctgaa
      421 gatctcgatg agatcgtgac ctcgccgaat gatgtgccca tcgacaacaa gctcgtggtc
      481 gccgcaacgc tggaaaacaa aaagatcgcg gacttttcct gccacggcgt aaccaaagtc
      541 gatgtcgcgg tgcccggcgt gaacatctat gccacgtatc cgaacggcaa gatgggcttc
      601 atgtccggca cttccatggc ctgtccgatg gctgcgcgct ttgccacgca ggtgctgcag
      661 gcgcacgacg gtctgaccgc ggtcgaagtg aagaagatcc tgatgggcac ggtcgacaag
      721 aaagactggc tggcagacaa ggtccgctcc agtggggtca tcaacccgaa ccgcgccatt
      781 cacgcggcca agctggtgaa atccggaaaa tctctcgaca ctgccatcgc cgaagccctc
      841 accgccgtga tggatgtgca accccgccgg tcgccgtata agggcccgaa cctcaaggac
      901 aagcaggttc gtgacttgta cttctcggcg attttctaat gcctgacacg cccgcagatg
      961 aggctggtac gagcctggtc tgcgggcttt tctctttcag acttcaaaca aggagctgtt
     1021 tcatgactct gtggcgcacc acctggttgt ctctgctcat tctcgccacc ctcgtcatcg
     1081 tgccggagct gcaggctcag gaactgacgc tgttcaccat gccgcctccg cgcagcctga
     1141 actgggcgtc cccctggaca ctcgcttttg atgccgccgt cggcaatcgg ttcaccttca
     1201 cccacatcaa acacaagcac accttcgggc acgtgttcat cgagctgaag gggccgaatg
     1261 gcgagcgcga actgaccggc agtaccaccg cccccgacgc cccgactgac tcagacaaag
     1321 tcaccaagga aggctacggc ctcggagtgt tctgggccga tctcaagggt gccctcgaaa
     1381 cgcccgcgag tctcgaccca cagattctcg accggtacaa atccggtcgc atcggcttca
     1441 tcacgttcaa gctgcacccg gatacgtacc agcgactcgc tcgttttctg cgggagtatc
     1501 gcgagcgtgg ctatggcaat atctacaatg gcatgaacct gccacgcgag gggcgagggg
     1561 ccggctgctc gattttcggc gtcgtgtttc tcgaactggc cgggttgctg caccccgagt
     1621 tcgtccgcga ctggatcatc tcggtcagag tgcccgaatc tctgatcggc gggccattga
     1681 ctggcaagaa gatctcgatc aagaatgcca ttctttcccg ttgggccaaa caggacgaga
     1741 aacaccgtgt tttgtcgctc tacgacccgg atctcatctt tgagtggata ctgaaagaat
     1801 gggaaaaagg gaagttcgat caacttcacg acacgtcttc agctgatcgc ggttacctca
     1861 gccgcaagca actggtacgc agacagaagg ccctgggtct gacgtatgac gtctctgaca
     1921 tcgcgccgcc aaccgacccg atctggcagg ggcaggccgc cgcgaaataa tcgaagaacc
     1981 gcaaccaaac ggggtgaact ctggttcatc ccgtttgttt tttacatgat acagtagaga
     2041 cgttcagatc tttttggagt gaggtgaaac ccatggcttc caacgcgctc aggcgtcaca
     2101 acgcctttct actcttcgtg tggctcgtcg ttttcgcgtg ttcggcgttc gtcccgggtt
     2161 ccgttctggc tcgcagtcac ctggaactgt cccctgactc agcaagagaa gttctgccgg
     2221 cggcccttcg cctgctggct caccaactgc gtcgcgacgg gtccctggcc ctcttcgact
     2281 tcgatggtac tctctgttct gaacagtatc tcgctccgga tggccgcttc cgctccgggc
     2341 aatccacctg gcatctctgg ggggctcgac atctgcagga atatccgcgc ctgtttctgc
     2401 agcagcggtt aggcttcgat cacgacgaag acgcgcgcct gatcagcgac aaagatgacg
     2461 ttctcgaggg gctgacggac atcgagagtc acgggtacat gaagttcgcc cagattgcct
     2521 gtttcgaagc cggcatgacc ccgggcgaac tgcacaccgg tctcgaaggt tttctgcagg
     2581 agtaccagcc ggcagattac gtgtatccgg tcatgatcga ttttctggag gtgctgttgc
     2641 gccaaggcac ccgcgtgtgg atcgtcagtg gcagcaaccc gcactacatt catgccgtgc
     2701 tgcgacaact ggcgcggcaa cgcggcgaag ccagcgagac cctgcttcga cacggacacc
     2761 tgatcgccga cacctattct ccgtatgacc cagctgctgg aaccggctgt cgcctcgtgg
     2821 gcaacatggc acgtcagggg cgcgatacca gatttcgcgc gatctacgac gaccggttca
     2881 cgccggccag cgggtctgcc cgcttcatcg tcgagagaga aggaaagtgg cgagcgtttg
     2941 agacgttcat caagccacga gatggagccg ccattggctt ggtcgcaggg aacagcgacg
     3001 gggattttga actgatcgac gcggccatga aagccaattc ccgcctgacc gcccttttgg
     3061 tcaacccgcg cggctcccac ctgaacacgc tgccgaagtc tcgttgtctc accatcaaac
     3121 agcagagtcg caaaggaggc gaaggcatga cacgagtcac cggcaccgct cagaatgcga
     3181 agctcgggcc actcgtcctc acgagcaacg ggcaagtctg ggttctcgga ctcgaggaat
     3241 ggccgcccga agccgtcggg caggaagtcg aggtctgcgg cacaaccgaa gagcgctctg
     3301 atcttccggt gtttgtccaa cctgtctcag gaccaatctc tcaagggatc ccggttgaac
     3361 ctggcacgga tctttctcaa gcggccaaac gcacctgtct cacgctggcg aactggcatc
     3421 tgcggtcgac tcaaccgaaa cgcggagcaa agccgtgaga acgctgtggt tcactctctt
     3481 tgttctgctg tccctcgctg cccccctcgc ggccgccgag ccacccgaca ctctgtacac
     3541 gctgccgtat ccgtcgggtc aggaatacct ggtctcggtt gcctacaacg actggcctac
     3601 ccacgtgggc aactatgcca tcgactggtt gatggatgaa ggcactcaca ttctcgccgc
     3661 gcgcgacggc gtggtagcga gagtcgtgaa cacgttcacc gaagccggtc tcacccccga
     3721 ctttctgcag aagaataacc tgctcacact gaagcaccct gacggtacgt acacagacta
     3781 cgttcatcta cagaagggcg gcatcaaagt gcgagtcgga caatcggtca aggccggcga
     3841 actcattgcc ttatcaggga ataccgggtt ttccagcgct ccccatctgc atttcattgc
     3901 ctatcggttg ggaaaaaacg gggcgcagga atcgtttccc gtgcgctttg tgtcaggcat
     3961 ggccaaaccc tacgagatct ttcgtgactg ccgctatctg gcgcccggcc ccgattgcca
     4021 gccgactgtc gatccattcg aggcggcctt tccgggcgaa ctggcgaagc tgaagttcaa
     4081 actggccgac atggcccggg cccaggtttc gccgcgcgca ggcgcgctgg ccgtgcgaga
     4141 ccatctgaaa gctcatggag ccgagtacaa gcagaagtat cgtgtcatct acgcggcagc
     4201 ccaggaagga gacagcgaaa gcctgcgtct gctgaccgcg ttcgcgagtt ccatggatct
     4261 gcgaactgat ccgaacatcg atcggctgtt ccaggattcc gccgccgccc cgattgcgga
     4321 agaagccctc gaactttgga atgagattca agtcatcgaa tagtcgcttt tcaggatggt
     4381 tttgaagttg atcatctagg acttctcgct gccggctcac cgaagtgtat tcgcacgcgg
     4441 tcgaggcatg aatctcgtga ggagcgctcg ccactctgat caatttctgt tactgtagta
     4501 gcaatttcaa atacattcag acaaaggaag gatgatatgg cgaagaagaa gatcacagtc
     4561 cttggggccg gaatggtggg cgcggttatg gctctcgatc tggcggccga agagcagttt
     4621 caggtaacga tcgctgatcg cgatcccgaa gccctgagcc gggttcgtgc caaggcacgc
     4681 ctcgaaacca tcgaagccga tctttcccgg gtcgaaaaga ttcgcgaact gatcaaggat
     4741 gccgacctcg tcgtcggagc ggtgccgggg tttcttggct ttcagactct gaaaaccgtc
     4801 attgagtgca agaagaactt ctgcgatatc gccttcatgc ccgaagattt tctgcagctc
     4861 gatgcgctgg ccaagcagaa cggcgtcacc gcgatcgtcg actgcggcgt ggccccgggt
     4921 atgtcgaatc tgattctcgg gaagtacggc agcgagtatc agaccttcga agaagcgctg
     4981 atcatggtcg gcgggcttcc ccgggtgcgc acctggccgt atgagtataa ggcgccattc
     5041 tcgccaatcg acgtcattga agagtatctg cgcccggccc gctatgtgcg cgacgggaag
     5101 atcgtcacca tgcccgcgct cagtgaaccc gagctggtca atctgcccgg cgtcggcaca
     5161 ctcgaagcct tcaacaccga tgggatgcgc tcactgctct tcacgatcaa atgccccaac
     5221 atgaaggaga agaccctgcg gtatccgggt cacatcgaga tcatgcgcat tctgcgcgag
     5281 acgggcttcc tgtcctatga ccccatcgag gtcaagggct ccaagattgc gccgatcgat
     5341 ctgaccacga aactgctctt ccccatctgg aaggcgaagc cgggggaaga ggagttcacc
     5401 gtcatgcgcg tgcagaccac cggcatacga gacggcaagc gaacgcggaa cacgtatgat
     5461 ctgctcgact tcacggatgc caaaaccggc ttctcatcga tggcccgaac caccggcttc
     5521 cccaacgtca tcatggcccg ccaggttctc agcggagcgt tcacgcgcac cggcgttttc
     5581 ccgcccgagt gtttcggcgg cgacaagaag gtctttcagg ctctcacgga cggcctcaaa
     5641 cagcgcggcg tcgtgttcaa ccaccgcgaa gaggttctct gaacaaggat cgatgcatga
     5701 tgccccagcc cctgcccaca caagcagggg ctggtttctt ttcatcaccc tcacattcgc
     5761 ttcacaccgt attctggaag gagtgaccca tgtctcatcg tatttcccct cgacggtttg
     5821 tcggttctgc gcttttcggc attctgatca tgaccgcgtg tctgatcacc cccacggtga
     5881 cggctgccga tcggcgtctc gacacttcga ttccctccgt ctcgacgagc gtacagggaa
     5941 acggagcgcg ggtggcgctt ctgatcggca ataccaacta tgatgaggct cctctcagta
     6001 atccggtgaa tgatgttcgc gcgttcgccg gcgcgctgaa aaagatcggc ttcgaggtgg
     6061 tggtgcgcga aaatgcgacc caccgcgaga tggatgaagc catcgtcgag ttctggaatc
     6121 gcctgaaggg caaggatgtc ggcctgtttt actactccgg acacggcatg caggtgaacg
     6181 gcgaaaacta cctggtaccg atcaaagccg tcgtgcgcac cgagatcgac attcgacacg
     6241 ggtgctatgc cgcacagaag atccttgatt acatgcagga tgccaagccg ggattgagtc
     6301 tgatcatcct ggacgcctgc cgcaacaatc cgtttcgtcg cagcatacgt tccgtggcgc
     6361 gcggtctggc accgatgacg acgggcaagc aggttctgct ggcatacgcg accgacccga
     6421 acaacgtggc ccaggacgga gaaccgcagg ggaactcgcc ttacatgaag cacatgctgc
     6481 gccatctcga gaagccgggc ttgccgatcg agaagttctt caaggaagtg cggaagggcg
     6541 tgttgctgga taccaacggt gaacagactc cctgggaaag cggcaatctg accgatgatt
     6601 tcgctttcgt gaccggtaca gattcgacct ccgacaaggg aagcgccgcg acgaccccgg
     6661 caaacgttga tccgaaacag aaggcctccc cgcgcgagct gtggacggcc gaagcagaaa
     6721 agggcaatgt cgatgccatg tatgtcgtcg cttataacta cgacagcggc agcatggatt
     6781 ttccggtgga tcatgtggcg gcgactgatt ggtacaagcg ggcggcggag nnnnnnnnnn
     6841 ggcggaggcc ggctcggccg ctgccatgaa agccatggcg gatcggttcg aagacggaat
     6901 cggctgtgag cagaacccga agacggctca cgcctggctg gaaaaggccg ccgccgcagg
     6961 agatactgcc gccgccacca aagtctcaga gcgcaacgcc cagctcacga acctgcgcga
     7021 gcgagccgcc aaaggcaacg gtttcgcgcg ttacgagctc tggcgtctcg aaaaccatat
     7081 ccccgaggac gtcagccgcc aggaacagct tctgcttcct gcagccgaaa aaggagatca
     7141 gaattcagcc gacctgcttt gcctcttgta cgttcgcgaa cttgaggtcg gcacccccga
     7201 agcccggcga acctggctga acaagtttgc cgccaaggga gacgccaacg tgaagtttct
     7261 gatcgccaag caatttcaga ccggcattgg cttgccgcgc gattttgagc gcgccgcgga
     7321 actgtaccgg cagtcacgcg atctggggca ccccaacgcc gcgcgagagc ttcaggaact
     7381 ctatgacgcc ggccaggccg acgcccagga ggacagcgga ctggcccaga cgctggatgg
     7441 gttgaagcag aaagccgaga gcggtgacgc cgcgtcccag ctgaagatgg cgatcaagct
     7501 ggccaaaggc gagggaacag ccatcgatat gccggcagcg atcacctgga ttcgcaaagc
     7561 tgcggcgcaa aactacccgc gcgcgatgac cagtctcgga cagctgcatc ttggcggtca
     7621 ggaaggcgtc aaggaagatc cgaccgaggg agtgcgtctg ttgaaacagg cagctgaagc
     7681 cggagacgcc gaggcagtcg tcacgctgat gaagtggcat cagtcgaaaa agccgggagc
     7741 cgacggtgat atcgaagccc tggcgtgggc gtacgtgctg atcaaacggt cagaaaccct
     7801 cggcgacatt gcctgggcct accgcgatgc cggcaacttt catgtcgaga aactcaccgg
     7861 cagcctgtcg acgggcgaaa tccaaaaggc tcgaaaacgt cagaacgagc tgctgaaaac
     7921 gatttcggcc ttcaaacgct tcgacgataa ctaacggttc aacggcaccg tgtggtctga
     7981 tggtcggtcg agatcgagat cggcaatcag cagatcagtc atttcgataa atgccggctc
     8041 gagctcaagc tcgcgctctg aaagcgcatc aagcgtcgat ccgcgacccg gaagatcgga
     8101 aacgatcagc agagccttgc accaggtctg tcgtccgagt cgagtatcga gaaactgagc
     8161 cagcgatgcc agttcgacat caaccacatc ggctccgagt tgtcgcaccg aggccagata
     8221 cggagcggtc tcctcgatcg gcgaaaagac gctgaagacg cgagccgggg tcagacgacc
     8281 cgacagccag cggggaattc gactccaggg ctgcac
//
LOCUS       DAORHD010000030         8278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8177_length_8278_cov_3.755077, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000030 DAORHD010000000
VERSION     DAORHD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8177_length_8278_cov_3.755077"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1775
                     /locus_tag="PKO06_01315"
     CDS             96..1775
                     /locus_tag="PKO06_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV68306.1"
                     /translation="MTAAAYLALAGINALTLAMPQLIRWIVDHGIVRRDATLLNGAVL
                     GLLALAFLRSGLAYLQGIWTETASQNVACDLRRELHRHLARLSFSWHDRAETGQLLTR
                     AIQDVERIRFVTGRAFLRILDGLLLLGGTAVMLVWMNPVLTLPALLTMPLLIWRALSL
                     GRRLRPISLAVQKQLSRVTTRLEQNLRGMRIVKAFAQEDAEITRFARENRRWRILANG
                     SARLTALNVPLIELIAGFGTILVVGCGGLLVMRQSLTLGELVAFTTYLGQLYMPTRRL
                     GMIVPAVSQAAAAGERVFEILDVIAEVRDAPDAVSVPTIRGQVRFEQVSFSYVRGHPI
                     LREVTFEVVPGQVVAVLGETGSGKSTIINLLPRFYDPISGRVLIDGYDIRHVTLASLR
                     AQIGTVVQETTLFAASVRENLLFGRPDASEAEMHAAAVAAQAHEFIMNLPNGYDTLVG
                     ERGVTLSGGQKQRIAIARTLLCDPRILILDDATASVDTGTEYQIQEALNHVMIGRTCF
                     IIAHRLSTLRRADLILVLDRGRLAAHGTHDELLRTSGLYADLCRQTQEMSP"
     gene            1772..3589
                     /locus_tag="PKO06_01320"
     CDS             1772..3589
                     /locus_tag="PKO06_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV68307.1"
                     /translation="MSFSLGSVGSGMGPRNAINEFGREGGGPVFDPRLLRRLLQLVQP
                     YRWQILAALAAMLVSSVLALATPWLIKNAIDRDIAGADATGLLQTVLLMMGAAVGAWA
                     ASSFQQYLLSNTGLLILADLRLRLFRHLQHLSLSYHHSHIVGVTISRVISDVAVIHDL
                     LTDGVATLIGDTIVLVGIMLVMLSLNVPLALVSFSVLPLMLLATWWFSRRARAAFRQT
                     RQRLAAVVGGLAEDISGIRVIQAFAREGVSAERFDEVNRANRDAYVEAMSLSFTFLPA
                     VDFLGVLAIAIVLWWGGAAVSGGGLTLGVVVAFLAYVTRFFQPIQELSQLYTTLQSAM
                     AGGERVFELLDTHPVIEDSPEAVPLPRVQGRVEFRHVGFEYQSGSPVLQDIQLTVDPG
                     KTVAIVGPTGAGKTTLASLITRFYDATTGTVLVDGHDVRTVTQHSLRSNTGLVSQDPF
                     LFSGTVADNIRFGHPQATLAEIQEASRAALAHEFITRLPAGYSTRILEGGVNLSIGQR
                     QLICIARAILADPRLLILDEATASIDTMTESLLQEALYRLLRGRTSIVIAHRLTTIRN
                     ADHIVVLQAGRIVEQGTHDELVARNGLYTELHQRQFLAS"
     gene            3672..4496
                     /locus_tag="PKO06_01325"
     CDS             3672..4496
                     /locus_tag="PKO06_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV68308.1"
                     /translation="MNHLSRLCVLFLFSCLPLLAAPCPRCQTSLPRQARFCPNCGQAF
                     VASAQPQKTPRVGREAVFQNFAVVDEFERVIKSNFYSAIVGGFPQFKVRLGNATRKFQ
                     AVRARLTPEVKILGDLYLEKAAVYEKIVSGLNTMRLDSMFRKALMIYYSRMIDWYNTL
                     LHWAREKSDYHAGDFAALQAAREDLKTKGRTYEVTAPYLKAGNTRLARGTPFAVLDVR
                     DGKALILALAETNTDEPVQRWESLNSLLERTTWSSNSEAVYRRSLSLKPESLAPGP"
     gene            complement(4500..7523)
                     /locus_tag="PKO06_01330"
     CDS             complement(4500..7523)
                     /locus_tag="PKO06_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNV68309.1"
                     /translation="MNLVRHCSHVMIRVFLVTTLWATAPALLAQATSGADTGMKGQAE
                     KKISELLGVPVTVRDYQLDYSTLRLKGVVIGDRTRKDQLQADIHEVSATCNFMSLLAG
                     SLVLEDVALASVSVRIPWSPAASLERVRPAASSTTRFDPATFPVKKVRGTSLEVRLDD
                     PRHQKSLALRLPAIEVTKADDLQAILIQLSGALRTISPKTGNAAPECSVRASIAVSGT
                     APFVGSATVDAGPAPVAEIQNLVEVFLPGSAAMVACKGGAAEANITFSQPGQKPGQIQ
                     AKLILRGLDLKPPTSGQSIRNLHGELRFNGTMQNGAANGVLDATRLRATVGTLGEISL
                     EKAKARFENFGAPAGTMHLEETVVAALGTTLRLSGSIKPSGTPQLRLLAGSKLDLADM
                     QKRLAGGGDPRWKQLTMAGTAELMVDVTGSLAAPIVRGGFQLSNGTIAIPERKIRLQN
                     TNLSISVDESGATIKTASGLIAGGQLTVTGTVKGQQQPVLNLNGSVKQAVANELLGLL
                     ASVAPSFPRDLKLSGPIDLEFGATGPLAKPVIQGACTLSQATLEMPVLMRPCTDLTGA
                     IQLTATGLQTKGLQARWGGSAFRVSGQVKDLSTFHTDLQFTVKPLDVTDLASFFLKDS
                     GYKVSGSGEAEGRMSGPAANLTITGTTRVPSGEVSMPLSSSNSSVYSAPYQNLSSGFR
                     YAGGKLAFNDAQASVFGGQLQGNCLIDPRARPIRFDLQLTGKALRTEAFLAENTSQKQ
                     VITGPVDGRFVATGDTTGLTSWNGSGSLSLRNGTYQAPPVVTPILSMLNLKQFASGDL
                     TSADGTFVLNSGIMTTQDLNFVTTIGKAFYRGDVGLDTTLRGDLNLVFSQAAVNQSQT
                     LKDLSLDGTSVSVPTKVRGTLLAPEFPGFSPGKLLELGLKRTGQKMLQEILTGKPKPA
                     DNSTATPTKKVDPAQDLLKELGGLFKKKKKKNTEAAPVPQTPAPAQPQPSVTSPAPAQ
                     VATSTAPAPAPAKKPQSIDKELKQLGKDLKKIFKF"
     gene            7871..>8278
                     /locus_tag="PKO06_01335"
     CDS             7871..>8278
                     /locus_tag="PKO06_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease domain-containing protein 2"
                     /protein_id="HNV68310.1"
                     /translation="MPTREEIVLLEPFCGLTIEQIQVPVTDDQFAAAVSEIQAAGAVG
                     FDTETKPVFVRGATNEGPDLVQFATEHRAFLFQLHRLVCRPYLIELLQSVAVMKIGFD
                     LTSDRSLLQHKLGVETQGIIDLSRAFRQRGYRQT"
BASE COUNT         1478 a         2433 c         2488 g         1879 t
ORIGIN      
        1 atgagaatct gttcatgtgc cgcacggagc ttgcccgacc gggcatggca gagacgttgc
       61 gccggtgttt cgggtatctg cgcccgtatt ggggcatgac agcggcggca tatctggcgc
      121 ttgcgggcat caacgctttg accctggcga tgccgcaact catccgctgg atcgtcgatc
      181 acgggatcgt cagacgtgat gcgactctgc tcaatggggc ggttctcggt ctgctggcgt
      241 tggcgttcct cagaagcggt ctcgcctatc tgcagggcat ctggaccgaa acggcctcgc
      301 agaatgtcgc ctgtgatctc cgtcgcgagc tgcaccgcca tctggcccga ctctcgttct
      361 catggcacga tcgcgccgaa accggtcaac tgctgacccg tgccattcag gatgtcgagc
      421 gcattcgctt tgtcaccggt cgcgccttcc tgcgcattct cgacggtctg ttgcttctgg
      481 gtggcaccgc ggtcatgctg gtctggatga acccggtcct gaccctgccc gcactgctta
      541 ccatgccgtt gctgatctgg cgggcgttgt cacttggccg tcgactgcgt cccatttcgc
      601 tggcggttca gaagcagctg tcccgggtca ccacccggct cgaacagaac ctgcgtggca
      661 tgcgcattgt caaagccttt gctcaggaag acgccgagat cacccgcttt gcccgcgaga
      721 accggcgctg gcggattctg gcgaacggct cagctcgact cacggctctc aacgtgccgc
      781 tgattgagtt gattgccggg tttggtacga ttctggttgt gggttgcggc ggtcttctgg
      841 tcatgcgcca gtctctgact ctgggcgagt tggtcgcttt caccacgtat cttggccaat
      901 tgtacatgcc gacaagacgt ctgggcatga tcgtaccagc ggtatcgcag gctgctgccg
      961 cgggtgagcg tgtgttcgaa atccttgatg tcatcgcgga ggttcgcgat gcgcccgatg
     1021 ccgtttcagt gcccacgatt cgcggtcagg tgcgcttcga gcaggtttcc ttctcgtacg
     1081 ttcgcggtca tcctattctg cgcgaggtaa cgtttgaggt ggtgcctggt caggtggtgg
     1141 cggttcttgg cgagacaggt tccggtaagt cgacgatcat caatctcctg ccgcgtttct
     1201 atgatccaat ctcgggaaga gtcctgatcg acgggtacga tatccggcac gtgacgctcg
     1261 cgtcactgcg cgctcagatc ggtacggtag tgcaggaaac caccctgttc gcggcttcgg
     1321 ttcgcgagaa cctgttgttc ggccgccctg acgcgagtga ggccgagatg cacgcggctg
     1381 ccgttgccgc tcaggcgcac gagttcatca tgaacctgcc gaacggatat gacacgctgg
     1441 tcggtgagcg cggtgtcacg ctctcgggcg ggcagaaaca acgcatcgcc atcgctcgca
     1501 cgcttctgtg tgaccctcga atcctgattc ttgacgatgc caccgcgagc gtggataccg
     1561 gaaccgagta tcagattcag gaagcactga atcacgttat gatcggcaga acctgtttca
     1621 tcatcgccca tcgactgagc acattgcgac gtgctgatct gatcctggtg ctcgatcggg
     1681 ggcgactggc ggcgcacggc acgcatgatg aactgctgcg gacatcggga ttgtatgctg
     1741 acctctgccg gcagacgcag gagatgtcgc catgagcttt tccctgggtt ccgtcggttc
     1801 gggaatgggc ccgcgcaacg cgatcaacga gtttggtcgt gaaggtgggg ggccggtgtt
     1861 tgacccgcgc ctgctgcgcc gattgctgca actggtacaa ccctaccgat ggcagattct
     1921 ggcggctctt gccgcgatgc tcgtttcgtc cgtgctggcg ttggccactc cgtggctgat
     1981 caagaacgcc atcgatcggg atatcgccgg tgctgatgcg accggcctgc tgcagactgt
     2041 tctcctgatg atgggggcgg ccgtcggagc ctgggctgcg tcctcgttcc agcaatatct
     2101 gctgtcgaac accggcctgc tgattctggc cgatctgcgt ttgcgcctct ttcggcatct
     2161 gcagcatctc tcgctctcct atcatcactc gcatatcgtc ggtgtcacga tctcgcgggt
     2221 gatcagcgac gtcgcggtca ttcacgacct gctcaccgac ggcgtggcca ccctgatcgg
     2281 tgacacgatc gttctggtgg gcattatgct ggtcatgctc tcgctgaatg tgcctctggc
     2341 cctggtctcg ttctctgtgt tgccactcat gctgctggcg acgtggtggt tttctcgacg
     2401 agcccgtgcc gcgttccgac agacccggca acgtctggcg gccgtggtcg gcggtctggc
     2461 ggaagatatc tccggcatcc gggtgataca ggcctttgcg cgtgagggcg tctctgctga
     2521 acgcttcgat gaggtcaatc gggccaatcg cgatgcctat gttgaggcca tgtcgctgtc
     2581 gttcaccttt ctgccggccg tcgattttct cggcgtactg gcgatcgcca tcgtcctctg
     2641 gtggggcggt gcggcggtgt ccggaggggg actgacgctc ggagtggtcg tggcctttct
     2701 ggcgtatgtc acccgattct ttcagccgat tcaggaactg tcgcagctct acaccacact
     2761 gcaatcggcc atggctggtg gtgaacgtgt ctttgaactg ctcgacaccc acccggtcat
     2821 tgaggacagc cctgaagccg ttccgctgcc gcgggtgcag ggacgggtcg agtttcgtca
     2881 cgtcgggttc gagtatcagt ccggatctcc ggttttgcag gatattcagc tgacagtcga
     2941 tccgggaaag accgtggcga tcgtcgggcc cactggcgcg ggcaagacca cgctggcgag
     3001 ccttataacc agattttatg acgccaccac tgggactgtt ctcgtcgacg ggcatgatgt
     3061 gcgaaccgtg acacaacatt ctttacggtc aaataccggc ctggtatctc aagatccgtt
     3121 tttgttttct ggtactgttg ccgacaatat ccgtttcggt catccccagg ccaccttggc
     3181 agagattcag gaggcatcac gcgcggcgct ggcgcacgag ttcatcaccc gactgccggc
     3241 ggggtattcc acgcgcattc tcgaaggcgg tgtcaacctt tcgatcgggc agaggcagtt
     3301 gatttgcatt gcccgggcga ttcttgccga cccgcgtctg ttgatcctcg atgaagcgac
     3361 cgccagcatc gacacgatga ccgagtcact cctccaggaa gcgctctatc ggctgcttcg
     3421 cggacgaacc tcgattgtca tcgcgcaccg cctgacgacg atcagaaacg ccgatcacat
     3481 cgtcgttctc caggccggtc gcatcgtcga gcagggaacg catgacgaac tggtggctcg
     3541 gaacggtctg tataccgaac tccaccagag acagtttctc gcgtcgtaaa acatcctgac
     3601 aacttgggag gttgccggat gcagggcgat cgagaggtgc ggatcatggt atgattttgg
     3661 tggaggacgt catgaaccat ctgagccgtc tgtgtgtcct gtttctcttc tcatgtctgc
     3721 cccttctggc cgcgccctgc cctcgctgcc agacgtccct gccccgtcag gcgcgatttt
     3781 gtccgaattg cggtcaggct tttgttgctt ctgctcagcc tcagaagact cctcgagtgg
     3841 gtcgcgaagc ggtctttcag aattttgccg ttgtcgatga gtttgaacgg gttatcaaga
     3901 gcaatttcta cagtgcgatt gtcggtggtt ttccgcagtt caaggttcgt ctgggcaacg
     3961 cgacccggaa atttcaggcg gtgcgcgccc gcctgacacc ggaagtgaag atactgggtg
     4021 atctgtatct ggaaaaagcc gccgtgtacg aaaagatcgt gtccggattg aacacgatgc
     4081 gccttgactc gatgtttcgt aaggcactca tgatctacta ttcccgcatg atcgactggt
     4141 acaataccct cctgcattgg gcgcgcgaaa aatccgacta tcacgcgggt gattttgcgg
     4201 ctcttcaggc tgctcgcgag gatctcaaga cgaagggacg cacgtatgaa gtgacggctc
     4261 cctatctcaa ggccggcaac acccggctcg ctcgcggaac gccgtttgcc gttctggatg
     4321 tgcgtgacgg caaagccctc attctggcac tggccgaaac caatacggac gagccggtgc
     4381 agcgttggga aagcctgaac tctctgcttg agcgtacaac ctggtcgtca aacagcgagg
     4441 ctgtgtatcg acgttcgctg tcgctgaaac cggaatcgtt ggcgccgggc ccgtagagat
     4501 cagaatttga agatcttctt cagatctttc cccagctgct tcagctcctt gtcgatggat
     4561 tgcggcttct ttgccggcgc tggtgccgga gcggttgagg tcgccacctg agctggagcc
     4621 ggagaggtca ccgaaggctg tggttgcgcc ggggctggcg tttgcggcac gggggctgcc
     4681 tcggtgtttt tcttcttctt tttcttgaag aggccgccca gctctttcaa gagatcctga
     4741 gcggggtcca ctttcttggt cggtgtcgcc gtcgaattgt cggccggctt gggtttgccc
     4801 gtcagaatct cctgcagcat cttctgaccc gtgcgtttca agccgagttc gagcagtttt
     4861 ccggggctga accccggaaa ctcaggtgcc agaagcgttc cacgaacctt ggttggtacg
     4921 ctcaccgagg taccgtcgag tgacagatcc ttgagtgtct gactctgatt caccgcagct
     4981 tgagagaaga ctaggttcag gtcaccgcgc aaggtcgtat cgagcccgac atcgccgcgg
     5041 tagaaagctt tgccgatggt cgtcacaaaa ttcagatcct gtgtggtcat gatgccgctg
     5101 ttcagcacaa aggtaccgtc agcgctggtc agatcacctg aggcgaactg tttcagattc
     5161 agcatcgaga gaatcggggt gaccaccggg ggtgcctggt aggttccgtt gcgcaggctc
     5221 agactgccgc tgccgttcca tgaggtcagg cccgtcgtgt caccggtcgc gacgaaacgt
     5281 ccgtcgacgg ggccggtgat cacctgtttc tgactggtgt tttccgcgag aaacgcctcg
     5341 gtacgcaatg ccttgcccgt taactgcaga tcgaatcgaa tcggcctggc gcgcgggtcg
     5401 atcaggcagt tcccctgcaa ctgccccccg aacacacttg cctgagcatc attgaaggcc
     5461 agtttgccgc ccgcataacg aaaaccactc gacaggttct gatagggtgc ggaatagaca
     5521 ctggaattgg acgaggaaag tggcatgctc acctcgcccg atggcacgcg ggtggttcct
     5581 gtaatcgtca ggttcgcggc ggggcctgac atccggcctt cggcctcacc gctgcccgaa
     5641 accttgtagc cgctgtcttt gaggaaaaac gaggcgagat cggtgacatc cagaggcttc
     5701 accgtgaatt gcagatcggt gtgaaacgtg ctcagatcct tgacctgacc gctgactcga
     5761 aaggccgaac cgccccaccg tgcctgcaga cctttcgtct gcagtcccgt cgctgtcagt
     5821 tgaatggcgc cggtgagatc cgtgcagggg cgcatcaata ccggcatctc gagcgtcgcc
     5881 tgcgacaggg tgcatgctcc ctgtatcacc ggtttcgcca gtggtccggt cgccccaaac
     5941 tcgagatcaa tcggtccgga aagcttcaga tcacgtggaa acgacggagc gacgcttgcc
     6001 aggagcccga gcaactcgtt ggcaacagcc tgtttcacgg acccgttcag gttcagcacg
     6061 ggctgctgct ggccctttac ggttcccgtc acggttaact gtccaccggc gatgagccct
     6121 gatgccgtct tgatggtcgc accgctctcg tcgacactga tcgagaggtt ggtattctgc
     6181 agacgtatct tccgttcggg aatcgcgatg gttccattgc tgagttgaaa accacctcgg
     6241 acaatcgggg cggcgagact gccggtgacg tcgaccatga gctcggcggt tccggccatg
     6301 gtcagttgct tccagcgcgg atcaccgcca cctgccagtc gtttctgcat atctgccaga
     6361 tcgagcttgc tgccggccag caaccgcagc tgcggggttc ctgagggttt gatagagccc
     6421 gagagtcgca gggtggttcc cagtgctgca accacggtct cctcgaggtg catggtgccc
     6481 gcaggagcgc caaagttctc gaatcgggct ttcgctttct ccagactgat ttcgccgaga
     6541 gtgccgaccg tcgcacgcaa ccgtgtggcg tcgagcacgc cgttggcagc gccgttctgc
     6601 atcgttccat tgaagcgcag ttcaccgtgc agattgcgta tggattgccc cgatgtcggc
     6661 ggtttcaggt cgagaccgcg gaggatcagt ttagcctgta tctgccctgg tttttgtcca
     6721 ggttgcgaaa acgtgatatt cgcttccgcg gctccgcctt tgcaggcaac catggccgca
     6781 gaacccggca gaaacacctc gaccaggttc tgaatctcgg ctacaggcgc cggaccggca
     6841 tcgacagtgg ccgagccgac aaagggagcc gtgccggaaa cggcgatcga ggcacgcacc
     6901 gaacactctg gagccgcgtt tcccgttttc ggggagatcg ttcgcagggc tccggaaagc
     6961 tggatcagaa tcgcctgcag atcgtcggct ttggttacct cgatggccgg cagtcgcagg
     7021 gcgagagact tctggtggcg cgggtcatcc agtctgactt ccagactggt accacgcact
     7081 tttttcaccg ggaaggtcgc cggatcaaac cgggtggtgg aggaagccgc cgggcgcact
     7141 cgctcgaggc tggccgccgg cgaccaggga atccgcacgg aaaccgatgc cagagcgacg
     7201 tcttcgagca ccagactgcc ggccagcaga ctcatgaagt tgcaggtggc cgacacttca
     7261 tggatatcgg cctgcagctg gtctttcctg gtgcgatcac cgataaccac acccttcaag
     7321 cgcagagtgc tgtaatcaag ctggtagtca cgaacagtta cgggtactcc cagcaactcc
     7381 gagatcttct tctccgcttg tcctttcatg cctgtatcag cgccagaagt ggcctgcgcc
     7441 agcagagccg gtgcggttgc ccacagagtc gtgaccagaa acacgcgtat catcacatgg
     7501 gaacaatgtc tgaccaggtt cattacaagc tcctctcttg cggtcggcag gcagccgcac
     7561 ctcgtggcgc tctgcatcac gccatcgcgg ccactgtcag ttcagaaacg tgaacctgtt
     7621 cagtctacgc cgaaacctgc ggtacgtcat ctgtcacgac gaaccagaaa atatggctgt
     7681 ctcgctcgtg tttctgttgt tttgggggac accagtgagc ctgtcggtgc gtgcagaccg
     7741 ttgtgccgtg gcatcgacag tgctatgctt cagcggttcg cgtctgtcat ctcaaccact
     7801 gaagtcgaca tcaacggtga ctcaacctct tgatcctcgg cccgcaaagc cgagaaccat
     7861 ccacaagccg atgcccacga gagaagagat tgttcttctc gagccgtttt gcggcttgac
     7921 gatcgagcag attcaggtgc cggtgaccga cgaccagttc gcggccgctg tgtctgagat
     7981 tcaggctgcc ggcgctgtcg ggttcgatac cgagaccaaa cccgtttttg tccgtggagc
     8041 aaccaacgag ggccctgatc tggtacagtt tgcgaccgaa caccgggcgt ttctctttca
     8101 acttcaccga cttgtctgca gaccatacct gatcgagctg ctgcaatcgg tggcggtgat
     8161 gaagatcggt ttcgatctga cctctgatcg tagcctgctg cagcacaagc tcggcgttga
     8221 aacgcaggga atcatcgatc tcagccgtgc gttccgccaa cgggggtatc gtcagacg
//
LOCUS       DAORHD010000031         8249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8218_length_8249_cov_4.391018, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000031 DAORHD010000000
VERSION     DAORHD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8218_length_8249_cov_4.391018"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKO06_01340"
     CDS             <1..485
                     /locus_tag="PKO06_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68311.1"
                     /translation="SQVRGGMESAYLLRETADPIVFSGPGGSKETQTRGLLRDWALFS
                     QRGSFGYPELETVEVVPHDEILSGYYVRIAVLNAAATLAQITQVFAEFGVEIQKLMQT
                     GQQLEVSEPDGIVSEVVFFTRPVREGTINAVLQRIRDHVKLAGVKCCYRYESPTPRRF
                     "
     gene            494..1687
                     /locus_tag="PKO06_01345"
     CDS             494..1687
                     /locus_tag="PKO06_01345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNV68312.1"
                     /translation="MFRPGEVLVQKYGGSSLATPERILKVAERIADQIRAGRRVAIVV
                     SATGDTTDDLITLLQQVNRDADARELDQLMATGEIASCSLMASALKRCGMRAHSFNAF
                     NLDIQTDTLFGAAEIKRFGRMERLAETLRRGGVAVVAGFQGITPDGDLTTLGRGGSDI
                     TAVALARELGQKVCEKFTDEDGVYTADPRLVPTARKIWHLNYDEMETLAQYGNGILHP
                     RSISYARAAGIRIHVRSSFSHEEGTLVGPDGDATVPVKSIACDRKQALVEIRGIPIPE
                     RPALPTGFRISAHEWIPAGTGRLHGRFAFKTADTFDALPELWSAAATWQADEVVFSSR
                     LRFLTLVGCGFSQDASTAGQYQKVLEQCQIRPLLFQHDGLRVTVAVDEDAAETALQEL
                     HRSFP"
     gene            1778..3865
                     /locus_tag="PKO06_01350"
     CDS             1778..3865
                     /locus_tag="PKO06_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68313.1"
                     /translation="MKQQPYGNCLIQTGFSRVWRFSLLLIWLSMTVSQGWAFVDAGVD
                     ASKFPVEVYGDYLEYRHKANQVVVKGKAFITYKDMRIAGDTIQSNTKSEDIFAQGNVD
                     FWKGYDQTKGDFLVYNMKSGKGWMREASIRRNRNFFRAKEVYVSPTYSLAKEIMNTTC
                     DHEDHPHYRLMAERFEVIPGKESTIENLAFRWRGRTLYHKSLEHSVEKEKSQKFFSTR
                     QGVSQIDGFYLKFMTDMDFGPNMQGKFVYDHFEKRGYGFGFNGAFTQKENSNGTFSFY
                     NLQETMRNHSNVQMNLSHNYRFKRGDSLSTNLSYTGDKSGGYSENQDLNTQLNYSGQL
                     SFATVNAIFSKFYDIDGDKYEFDNGYQILDRLPEVNFSFPAYTLPGVPITMNLSGMFA
                     KYQEGTPDDRRNTEKKDIRSSFTTPTIQVNERFDLTPSYNFEKSWYSGGIIRETGTTN
                     VRASHKFTSMTNMEFNYNITTQKGESPFRFDSQTTVDLFSTRLRIATQTWSFNPINFN
                     YNRVAKRLEQVYWDYSLRSSPDAYHNWEFFLRRDYTPDPVALSKMAFTRLTPGNLNIR
                     YRLSSQLWSFDTSVTYPHEYRRITNTSFNYRATIRPLWQVSSNGNFNHLTGKFSPLTL
                     GIIRDLHCWETRAEYNLERKEFWIEFYLKAFPSDAGRFRYGADTNRLEAKLATFDQLT
                     QQYDSFRGPGGVR"
     gene            complement(3886..4686)
                     /locus_tag="PKO06_01355"
     CDS             complement(3886..4686)
                     /locus_tag="PKO06_01355"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HNV68314.1"
                     /translation="MNTFKRSLFVPGLIMLLMTGFCSAISAGEKNMPPVRDPKDYHPD
                     PYYMKLIANYPTLVERKIDRAMRKYLNEPQTLVLNFTPINETETLQGRYARIFVKVGR
                     GSADHLVVEDAEFEFLDVLLDINKLLREDKIDTVTVKSINMNVRITEADLNAFLRQKA
                     KKIKVDNPKAELKADSITVSGSTKYGFMKVVFWATGNFSVHQAREIYFYARKVKVNGM
                     TMPRGFVGSLVKQINPIMNLDKFPFQLNLQQIRIEPGLLLFTSSRAGN"
     gene            complement(4683..5600)
                     /locus_tag="PKO06_01360"
     CDS             complement(4683..5600)
                     /locus_tag="PKO06_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68315.1"
                     /translation="MSTVPQLEIEDLSRAWRRFALNLEPDDIPLLLCHMSASGFRRFQ
                     QTVRQPSILLWVPPEPPDSYVPTILPKSHHLYVDVLPVSTEAPLLVYAHRRGGGELDF
                     RSRHPRYRSVSHPQGHFDFDELTDRLQHASRQSLHVIPTPKHRSAVIAHWRPELVAAL
                     THSVYRSDLTLVQLLGDRPWKGRILYPETLFASLPAATIREYEFSGHDLQLYVERIFQ
                     SASPGRLGVAGGKLAFLAGKVRTLQGRDLPIQPGLSYRLSLDSSLYSVPSLHPESFGG
                     RRLGTAGHTFWSIALRQLPLLEPGKGLGQ"
     gene            complement(5711..6160)
                     /locus_tag="PKO06_01365"
     CDS             complement(5711..6160)
                     /locus_tag="PKO06_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68316.1"
                     /translation="MSTSRIAVKSYIHAVSLLLIFFSSATVLSGVTRGAIDFQLFFFG
                     NTRGQSVMFQTADDGAGAFSFAPIWKLPEIVNQFTRGYETRTLIINVGNNEHPAAPVS
                     YLLDEPIFEHRAGSAVPRSTPSGRATSLAPHSSAKFRSFSSAASGAT"
     gene            complement(6168..7247)
                     /locus_tag="PKO06_01370"
     CDS             complement(6168..7247)
                     /locus_tag="PKO06_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV68317.1"
                     /translation="MIKTLDRYIFMQLLEPFCFGFFLFVVVISIDPLISALQNIVNDN
                     VSAWSVIRWFMFRLPQDMIFTFPMSVLLSTLLVFGRMSKDSETVAMKAGGINFFRLLI
                     PVVMFSLTVTGVSFVFNELMVPYSNRRAQEIRRTEILRLVEPEAAENVIMRAADGAFV
                     YARKVFEKSQTMEKVLLEYYQDGKLQRRISADRAVYENKQWVLYETIDQKYLDGKVND
                     PTPVARYVAEGIREPIKEFARQTKRSSEMSFRELKGRIDLFERANFMETTDLKVELAL
                     KTSLPFASFFFALIGASFGLTKYRSGAFIGFGVSIIIIFIYYVLMSIFCAMGKSGYLP
                     VYLSAWLQNIICLFVGIHIVRQVNT"
     gene            7343..>8249
                     /locus_tag="PKO06_01375"
     CDS             7343..>8249
                     /locus_tag="PKO06_01375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNV68318.1"
                     /translation="MRVSVIGTGYVGLVTGACFAESGNEVICMDTDKRKIDMLLDGKI
                     PIFEPGLDELVKRNAAEERLKFSTSMDDAVRHGLFQFIAVGTPPGEDGSADLQYVLTV
                     AREIGKRMTEYKVIVDKSTVPVGTADLVRAAVKDELKKRNAEIPFDVVSNPEFLKEGN
                     AIDDFMKPDRVVVGCDDVRTAELMKELYAPFVRTEKPILVMDVKSAEMTKYAANCMLA
                     TKISFMNDIANLCDLVGADVDSVRKGIGSDSRIGYSFIFPGAGYGGSCFPKDVKALLQ
                     KGKQMGYDMEIIDAVERVNERQKMVL"
BASE COUNT         2003 a         2094 c         2335 g         1817 t
ORIGIN      
        1 tctcacaggt tcgcggtggc atggagtccg cctatcttct gcgagagacg gctgatccga
       61 tcgtcttttc cggcccgggc ggcagcaagg agacacagac tcgtggtctg ctgcgtgatt
      121 gggccttgtt ttcacagcgc ggttcgttcg gctatcctga actcgaaacg gttgaggtag
      181 tgccgcacga cgagattctc agcggctact acgtacgtat tgccgtgctc aatgccgcgg
      241 cgacattagc tcagattacg caggtattcg ccgaattcgg cgtggagatt cagaagttga
      301 tgcagaccgg tcagcagctt gaggtttctg aacctgatgg catcgtcagt gaggtcgtgt
      361 tctttacccg cccggtacgc gagggaacaa taaatgctgt cttgcagcgc attcgcgacc
      421 acgtgaaact tgccggggtg aagtgctgct atcgatatga aagccccacc cctcggagat
      481 tctgaaacgg tctatgtttc gacccggtga agtgctggtc cagaaatacg gtggttcgtc
      541 gctggcgact cctgaacgca ttctgaaggt ggcggagcgg attgccgatc agatcagggc
      601 cggccgacgt gtggccatcg tggtctcggc gaccggagat acgaccgatg atctgatcac
      661 cctgctgcaa caggtgaatc gcgatgccga cgcgcgcgaa ctcgatcagc tcatggcaac
      721 cggtgaaatc gccagttgtt ctcttatggc cagtgccctc aaacgctgcg ggatgagagc
      781 acactcgttc aatgccttca atctcgacat tcagaccgac acgttgtttg gagccgctga
      841 aatcaagcga tttggtcgca tggaacggct ggccgaaacg ctccgacgtg gcggagtagc
      901 cgtggttgcc gggtttcagg gaatcactcc cgatggagat cttacgactc tcggtcgcgg
      961 cggttctgac attacggcag tcgcattggc acgcgaactc ggacagaaag tctgtgagaa
     1021 gttcaccgat gaagatgggg tatataccgc tgatccgcga ctggtgccca cggcacgcaa
     1081 aatctggcat ctgaattatg acgagatgga aaccctggct cagtatggca acggaattct
     1141 gcaccctcgc agtatcagtt atgctcgtgc cgcaggcatt cgcatccatg tacgctcgag
     1201 tttctcacat gaggaaggca cgctggtcgg ccctgatgga gacgcgaccg taccggtgaa
     1261 aagtatcgcc tgtgacagga aacaggcgct ggtcgagatt cgcggaattc cgattcccga
     1321 gcgcccggca ttaccgacgg ggtttcgcat ctcagcccac gaatggattc ctgccggcac
     1381 ggggcgtttg cacggccggt ttgccttcaa gacggccgat acgttcgatg ccttgcccga
     1441 actctggtct gctgctgcga cctggcaggc cgatgaagtc gttttttctt cgcgactgcg
     1501 ttttttaacc ctggtcggct gcgggttttc tcaagacgcc tcgacggccg gacagtatca
     1561 gaaagttctc gaacagtgtc aaattcgtcc actgctgttt caacacgatg gcttacgcgt
     1621 gaccgtcgca gtagacgaag atgctgctga aaccgcttta caggaacttc accggtcctt
     1681 cccctgaaga atctcttctt caaattccgt aaaacgccga tacagatgat gcggcttttt
     1741 ctgtcaaagc tgtgggcgtt catttggaaa aaagacggtg aaacagcaac catacggcaa
     1801 ttgcctgata cagaccggtt tttcacgggt ctggcgattc agcctgcttc tgatctggct
     1861 gtcgatgaca gtctctcagg ggtgggcatt tgtcgatgcc ggagtcgatg cgagcaagtt
     1921 tccagtcgag gtctacggag actatctgga ataccgtcac aaggcgaatc aggtggtggt
     1981 gaagggaaaa gcgttcatca cctacaagga tatgcgtatc gcgggcgata cgattcagtc
     2041 caacacgaag tctgaagaca tctttgcgca ggggaacgtc gacttctgga aaggctatga
     2101 ccagacgaag ggcgactttc tcgtctacaa catgaaatca ggcaaaggct ggatgcgcga
     2161 agcctcgatc agacgcaatc gcaacttctt ccgcgccaaa gaagtctatg tcagtccgac
     2221 atacagcctg gcaaaagaga tcatgaatac gacctgcgac catgaagacc acccgcacta
     2281 tcggctgatg gccgaacgtt tcgaggtgat tcccggtaaa gagtcgacga tcgaaaatct
     2341 ggcctttcgc tggcgtggtc gcacgctgta tcacaagtcg cttgaacatt cggtcgagaa
     2401 agagaagtcg cagaagttct tcagtacccg ccagggcgtt tcccagatcg acggcttcta
     2461 tctgaagttc atgaccgaca tggatttcgg ccccaacatg cagggaaaat tcgtctatga
     2521 ccatttcgag aagcgtggat acggctttgg attcaacggc gcgttcacgc agaaagagaa
     2581 cagcaacggc acattctcgt tctacaatct gcaggaaacg atgcggaatc acagcaatgt
     2641 gcagatgaac ctgagtcaca actatcgctt caaacgaggg gactcactgt ctaccaatct
     2701 gagttacacc ggtgacaaat caggcggtta ctctgaaaac caggatctga atacgcagct
     2761 gaactacagc ggtcaactgt cgtttgccac ggtgaatgcc attttcagca agttctatga
     2821 cattgatggc gacaagtacg aattcgataa cgggtatcag attctcgatc ggttgcctga
     2881 ggtcaacttc tcgtttccgg cctatacgtt gcctggcgtg ccgatcacga tgaatttgtc
     2941 ggggatgttc gcgaagtacc aggagggaac tccggacgat cgccgaaaca cggagaaaaa
     3001 ggacattcgc agcagtttta cgactccgac gattcaggtg aatgaacgtt ttgatctgac
     3061 accttcgtat aacttcgaaa agagctggta cagcggcgga atcattcgcg aaactgggac
     3121 aaccaacgtt cgggcgtcgc acaagttcac gtcgatgacg aacatggaat tcaactacaa
     3181 catcacgacg cagaaaggtg aatcaccgtt ccgattcgat tcgcagacga cagtagatct
     3241 gttctcgacg cggttgcgca ttgcgacgca aacctggagt ttcaacccga tcaacttcaa
     3301 ctacaatcgc gtggcaaagc gactggaaca ggtctactgg gattacagcc tgcgctcgag
     3361 cccggatgcc taccacaact gggaattctt tctacgacgc gattacacgc ccgacccggt
     3421 cgcactctcg aagatggcct tcactcgcct gacaccgggg aatctgaaca ttcgctatcg
     3481 attgagttcg cagctctggt cgttcgatac gagtgtcacc taccctcatg aataccggcg
     3541 tatcacgaac acgtcgttca actatcgtgc gacgattcgg ccgttgtggc aggtcagctc
     3601 gaacggaaac ttcaatcacc tgacgggcaa gttcagcccg ctgaccctcg gaatcatccg
     3661 tgatctgcac tgctgggaaa cacgggcgga atacaatctg gagcgcaagg aattctggat
     3721 cgaattctat ctgaaagcgt tccccagcga tgctggcaga ttccgatatg gtgctgatac
     3781 caaccggctg gaagcgaaac tggcgacatt tgaccagctt acgcagcagt atgacagctt
     3841 ccgtggtccc ggaggagttc gttaagcagc tcgaattccc gtttctcagt tcccggcccg
     3901 tgaactggtg aacaacaaga gacccggttc gatgcgtatc tgctgcaggt tcagctgaaa
     3961 cggaaatttg tccaggttca tgatcgggtt aatctgcttc acaagcgaac ctacaaaccc
     4021 acgaggcatc gtcatgccgt tcactttcac tttccgggca tagaagtaga tctcacgcgc
     4081 ctgatgcacg gagaagtttc cggttgccca gaagaccact ttcatgaatc cgtatttggt
     4141 tgaaccggaa acggtgatcg aatcagcctt cagttccgct tttggattat cgaccttgat
     4201 cttcttcgct ttctgtctga gaaacgcgtt gaggtcggct tcggtgattc ggacattcat
     4261 gttgatgctt ttcaccgtga cggtatcgat cttgtcttcg cgcaaaagct tgttgatatc
     4321 gagcagtaca tcgaggaact cgaactcggc gtcttcgacg accagatgat ccgcggagcc
     4381 acgaccgacc ttgacgaaga tgcgcgcgta gcggccctgc agggtctcgg tctcgttgat
     4441 tggggtgaag ttcagcacca atgtctgcgg ttcattgaga tacttgcgca tcgctctgtc
     4501 gatcttgcgt tcgaccagag tcggatagtt ggcgatcagt ttcatgtagt agggatcggg
     4561 atggtaatcc ttcgggtcgc gtaccggtgg catatttttc tcaccggcgc tgattgctga
     4621 gcagaagccg gtcatcagca acatgatgag acctggcacg aagagagaac gtttgaaagt
     4681 gttcattgac ctaatccttt tccaggctcg agaaggggca actgccgcag ggcgatgctc
     4741 cagaacgtgt gtcccgctgt accaaggcgc ctgccgccaa acgattccgg atgcagtgat
     4801 ggcacagaat acaacgaaga atcaagcgac aggcgatagg acaaacctgg ttgaatcggc
     4861 agatcccttc cctgcaaggt tctcactttt cccgccagaa atgcaagctt gccaccggcg
     4921 acgccgagtc gacccggaga agcgctttga aaaatgcgct cgacatacag ctgaagatcg
     4981 tgtcccgaga actcgtattc gcgaattgtg gcagctggta aagaagcaaa gagggtttcc
     5041 gggtagagga tgcggccttt ccagggtcga tcacccagca gttgaaccag ggtaagatcg
     5101 gaacgataga cgctatgggt cagagctgcg accagttcag gccgccagtg ggcgataacc
     5161 gcggagcggt gtttcggtgt cggtatgaca tgcagggact gccgtgaagc atgctgcagc
     5221 cgatcggtga gttcgtcgaa atcgaagtga ccctgcggat gtgagacgct gcgatagcgc
     5281 gggtgacggc tgcgaaagtc gagttcgccg ccaccgcgac ggtgcgcata gaccagcaat
     5341 ggagcttcgg tcgagaccgg aagcacatcg acgtagagat gatgcgattt gggaagaatg
     5401 gttggaacgt aggaatcagg gggttcgggt ggtacccaca ggagaatgga tggttgtcga
     5461 acggtttgct gaaatcgtcg aaacccggat gcggacatgt ggcagagtag caggggaatg
     5521 tcgtcgggtt cgagattcaa ggcaaagcga cgccaggcgc gtgagagatc ctcgatttcg
     5581 agttgcggta cggtggacac gaaagaatcg gtgagtaatt ctggttcgat gaaacaaccg
     5641 aacacaagtt ggtgaccgct gagaagatgc cgatcgtact ggcgaaagaa gggttgttcc
     5701 gagggctgaa tcacgttgct ccagaggcgg ccgaagagaa actccggaat tttgccgagg
     5761 agtgcggcgc gagcgaagtc gcccggcccg agggcgtcga cctggggacg gcagaaccgg
     5821 cgcggtgctc gaaaatcggc tcatcaagca gataactgac gggagccgcc ggatgctcat
     5881 tgttgcccac gttgatgatc agggttctgg tttcgtagcc gcgcgtgaac tggttgacga
     5941 tctccggcag cttccagata ggggcgaacg aaaaagctcc cgcaccgtca tcagctgtct
     6001 gaaacatcac gctctgcccg cgggtattgc cgaagaagaa cagctgaaaa tcgatggcac
     6061 cacgagtgac accagagaga accgttgcac tgctgaaaaa tatcaacagc agagatacag
     6121 catgaatata ggatttcacg gcaattcggc tggtactcat gacaatttca ggtattcact
     6181 tgtcttacga tgtgaatgcc gacaaacagg cagatgatgt tttgaagcca ggcagagaga
     6241 tagaccggca gataacccga cttgcccatg gcgcagaaga tactcatcag cacgtagtag
     6301 atgaagatga tgatgatcga taccccgaaa ccgataaagg ccccgctgcg gtatttggtc
     6361 aagccgaagg aagccccgat cagggcaaaa aagaaactgg caaacggcag cgatgttttc
     6421 agtgccagtt ccaccttgag atccgtggtt tccatgaagt ttgcacgttc gaacagatcg
     6481 atgcggccct tcagctctcg gaaactcatc tcggaggaac gcttggtctg ccgggcgaac
     6541 tccttgatcg gttcacgaat tccctcggcg acataacgcg cgactggagt cggatcattg
     6601 acttttccgt caagatactt ctgatcgata gtctcatata gtacccattg cttgttttcg
     6661 taaaccgcac gatcagcaga gattcgtcgc tgtaactttc catcctgata gtattcgagc
     6721 agaacctttt ccatggtctg gctcttctcg aacactttgc gggcgtagac aaatgcgcca
     6781 tcagcagctc tcatgatcac gttttccgca gcttcgggtt cgaccagacg cagaatctcg
     6841 gttcgacgaa tttcctgggc gcgacggttt gaatacggca ccatcagctc gttgaaaacg
     6901 aaactgaccc cggtgacggt cagtgagaac atgacgaccg gaatgagaag tcgaaagaag
     6961 ttgattcccc cggccttcat ggcaacggtt tccgagtctt tcgacatgcg accgaacacc
     7021 agcaaggttg acagcagcac cgacatcggg aacgtgaaga tcatgtcctg cggcaggcgg
     7081 aacataaacc agcgaatgac cgaccaggcg ctgacgttat cgttcacgat gttctgcaga
     7141 gccgaaatca gggggtcaat cgaaatgacg acgacgaaca ggaaaaagcc gaaacagaat
     7201 ggctcgagca gctgcatgaa gatatagcgg tcgagtgtct tgatcatcgg gtcaatgcct
     7261 gtctgccgaa aattggaaaa gcatgttcgt ggtggtatta tgacctcccc aacaggaata
     7321 ttcaaccgca gggagagaca agatgcgagt cagtgtgatt ggtaccggat atgtcggtct
     7381 ggtgacggga gcttgttttg ccgagtcggg caatgaagtg atctgcatgg ataccgacaa
     7441 aagaaaaatc gatatgctgc tcgatggcaa gattccgatt ttcgaaccgg gtctcgatga
     7501 actcgtcaag cgcaatgccg ccgaagagcg gttgaaattc agcaccagca tggatgacgc
     7561 ggtcagacat gggttgttcc agttcatcgc ggtgggcaca ccacctggtg aggatggttc
     7621 tgcggacttg cagtatgttc tgaccgtggc ccgcgagatc ggcaagcgca tgaccgaata
     7681 caaggtcatt gtggataagt cgacagtacc agttggaact gctgatctcg tgcgtgcagc
     7741 cgtaaaagac gagctgaaga aacgtaatgc tgaaattccg ttcgacgtgg tgagcaaccc
     7801 tgaattcctc aaagagggaa atgcgatcga cgacttcatg aaacccgatc gcgttgtcgt
     7861 cgggtgtgac gatgtgcgca ccgccgagtt gatgaaagaa ctctatgctc cctttgtccg
     7921 caccgaaaag cccattctgg tgatggatgt taaatcagcg gagatgacca agtatgcagc
     7981 caactgcatg ctggcgacca agatttcgtt catgaatgac atcgcgaatc tttgtgacct
     8041 ggttggcgct gatgtcgact cggttcgcaa aggaatcggc tcggactctc gcattggcta
     8101 ctcgttcatc ttccccggag ccggatacgg cggaagctgt tttccgaaag acgtcaaagc
     8161 gcttctgcag aagggcaagc agatgggcta cgacatggag atcattgacg ccgtcgaacg
     8221 cgtgaacgag cgccagaaga tggtgctgt
//
LOCUS       DAORHD010000032         8173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8330_length_8173_cov_4.317935, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000032 DAORHD010000000
VERSION     DAORHD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8173
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8330_length_8173_cov_4.317935"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="PKO06_01380"
     CDS             complement(<1..2331)
                     /locus_tag="PKO06_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV68319.1"
                     /translation="MVKRFYLEEIIDRSHLSAFHQRFSKLTGIRFVPFNERGEAVIGD
                     PQELLGRMDAIDFEQLARRDFMTRPDGSCIRVIKLTARDHLFGAVLLGPYLEANAKAQ
                     IAGVPRLTEDQVQAAVELIEQFFATQLEVSVERESIQRKEKILSVLEEASNIMNSSLE
                     FNNLLEFLMDIAIDITGATCGAILLKKESKKYLEVAAARGKYPQEVKKIRVPLGKGVT
                     GWVAKNREPLNVPNVLLEPRYIETPETIYSEMAVPLMVGDHLLGVIAVDSSEMNAFSK
                     EDCATLITLASMVTKVVENSRLLSNSNRKLNELTRLYSISERLAEQPEDTELLDRVMA
                     DAAAALGCKGMALMRYDSDKEDVYSICNYSLPEELHGLRVPLGKGIHGWVAQHRQPLL
                     VADAAQDVTVARHSFFDTFSRSVLCVPLLAGDRLVGVLSAHQKGDGQPFIEDDQRLLV
                     AIGRLLTAHFENRRLIDDSRRKLDYLTTLYKVSSSVAGTLNVNRLFNMIMHQVQEVMD
                     VENVSLMVHDSIKGQLSLDAAIGIPKKWVGTSTVKVGEGIAGWVAKHRQPLLIKDIAK
                     DERFLNRPSRMDYKTRSALSVPIINNRSLLGVLNVNNKRSGGIFSEDDLNLLLGISGQ
                     ISHAIGNAKLYERTEQQVAELSLIQELGKAINSSLDLDSVLNYFIDMTTRITESDKSS
                     LMLFDEEQQNLYVQVHRGFDPNAPVKSFSFKLGEGIAGKSAEQRRPMRVINTLEDANY
                     KQLPFSSSQTPLTLIAAPLLNRDKLIGVINCERELTK"
     gene            complement(2548..3006)
                     /locus_tag="PKO06_01385"
     CDS             complement(2548..3006)
                     /locus_tag="PKO06_01385"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HNV68320.1"
                     /translation="MITLFRWLWKLIVLGSAFGIGYIIGREHSFEEEEEWEAEEEERK
                     EAEKKDAAKPADAAPAAPATGGAEKPVAFAYENAAAKTVSIVGDFNDWNKDKNPLTLE
                     NGVWKTSLMLKPGRHEYKYVVNHTDWITDPKGGETVADKYGGRSSVIDVK"
     gene            complement(3011..5560)
                     /locus_tag="PKO06_01390"
     CDS             complement(3011..5560)
                     /locus_tag="PKO06_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV68321.1"
                     /translation="MSPEEKALMQAIQGLNERQREAVELTEGPVLVIAGAGSGKTRTL
                     TVRIANLVLHRGVPAESILAVTFTNKAAREMRERLHALMPGDASRLTVTTFHSFCCLL
                     LRRWHKHAGFSDGFTIYDDDDQERLMKLVIKELKGDPKKFTPRFLLNLASQAKNDLIG
                     PDEYKPSGPDRDFLGAAYRLYQQKLREHQALDFDDLLMESWKLLTQKPELLEKVQKTY
                     QYFLVDEYQDTNFAQYKLVALLSERSRNLCVVGDDDQSIYGWRGATIRNILEFEKDFA
                     GARVVVLDQNYRSTQAILDAASALVAKNRSCRNKKLWTDRRGGDRLRFLLASDDREEA
                     DLVVREIMRLERDGFRLQDIAILFRMNSQSRSFEQTLVKNRLPYEMTGGVKFFARREI
                     KDVLAYLRVLANPSDAISWKRIINTPSRGIGEATVQSLEAEGVLAESIATRAKQKPES
                     KIAVFHALMQRLRTLLDEEGVFEAARGVVRLTGYLDYLEKDDPDTAEERRENVESLLS
                     DIRQQLELKPDLTLREYLEQTALHADVDDLDETANAVHLLTMHNAKGLEFPVVFVVGM
                     EEGIFPHRSSSGAQEEIEEERRLAYVGMTRAMKRLYLTAARRRMMFGTWQNNALSRFL
                     GEIPRESFEPGTMMPRVFGVSSGVDEDGEDVSIEHRLPAAGQVKPFGVPVRGGVSRGK
                     GPTFAPLARAAASRQTGTSAPFSGKALPYPPPTTAPVYQRDADKPPLAGSPYNQAPST
                     ARTKTAGDQSVAPRQAVPEPVAEPPTSVTAAPAAADRGRGETSTANMINCRPGVEVVH
                     PLFGRGRILSIEGASLADFRMIIDFPRSGRKTLLLQYANLRVINNANSRGG"
     gene            complement(5550..6806)
                     /locus_tag="PKO06_01395"
     CDS             complement(5550..6806)
                     /locus_tag="PKO06_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HNV68322.1"
                     /translation="MSWLVVASFYVFRVCCATKVVFRNELVQALRVDSRRERDRKGCE
                     MLLPDVGILSGELLHPLKDIREGEKITGGCGWGETATTSVSDIFVQFDHKGPVLTLKT
                     ARGAEVRLSPEHQCFCRLNPLSRHHYLYLMERSTVGFRVGMSQELMKELVAMQTAKPD
                     WGNHSEIVDRLWIIESTESLPKALFLQKYAETKYGLPGFPFSAKHALCELTDDLILEL
                     FNRIDTPSRARQLLLDSLMFEESPHVTFRYSRSNPAVSTAIQFVMYGSGERCRTNNFF
                     SHLIRIDGTVELKGAEGKQFKRRVNKAGLWNMEVSREDLEEAQLFVKTLACLDTLEIV
                     KKIQLSRKNPYYLMPASHVKIGMSVPVLDRQGIDDDIVVSVQSEQYQGPLYDLKIEGL
                     NNFVAGGWVVGAYAGPGKQVPMRDVS"
     gene            6976..7971
                     /locus_tag="PKO06_01400"
     CDS             6976..7971
                     /locus_tag="PKO06_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNV68323.1"
                     /translation="MPNTPVARPRRYQITAISDALTDIIMETPDEALAGLGLPKGNSI
                     ELNSENLQKLESLLLKHEPTISPGGSPTNVIYGAANLGLHCAFIGCVGSDDYGYDYIN
                     NLRENGIDTYVSIKKGKSGVCYTLITPDGQRTFGLDFGVTKMLHDYEILHSLVRDSQY
                     LHFSAYELRGDSPINKATRHAYQIARKHGTRISFDLGDSFVISKIRDKLFEFLNDKVD
                     ILFANEDEAVCLSQSRDFRRLLAFSDLVVIKLGERGSLALTPDNEVAIPAYKVDQVKD
                     TNGAGDNFQAGFFYGLFKGLPLETCLKIGNFLAASVIRRVGAQAKVKISGIEFFI"
BASE COUNT         1779 a         2398 c         2196 g         1800 t
ORIGIN      
        1 cttggtcagt tcgcgttcgc agttgatgac cccgatcagc ttgtcgcggt tcaacagcgg
       61 cgcggcaatc aacgtcagcg gggtctgcga ggaactgaac ggcagttgct tgtagttggc
      121 gtcttcgagg gtgttgatga cacgcatcgg tcggcgctgc tcggccgact tgccggcaat
      181 gccctcaccc agcttgaatg agaagctctt caccggcgca ttcgggtcga acccgcgatg
      241 cacctgaaca tacaggttct gctgctcctc atcgaagagc atcagcgacg atttgtcaga
      301 ctcggtgatg cgggtcgtca tgtcgatgaa atagttgagc accgagtcga gatcgagtga
      361 cgagttgatc gctttcccga gttcctgaat caggctcagc tcggccacct gctgttcggt
      421 gcgctcgtac aatttggcgt tgccgatcgc atgcgaaatc tgcccggaaa tgcccagcag
      481 cagattcaga tcgtcttccg aaaagatgcc gccggaccgc ttgttgttga cgttcagcac
      541 gcccaacaaa ctgcgattat taataatcgg aaccgacagt gctgatcggg ttttgtaatc
      601 catgcgactc ggacgattga gaaatctctc gtccttcgcg atatctttga tcagtaacgg
      661 ctggcgatgt ttggcaaccc agccggcgat gccttcgccg accttgacgg tcgaagtacc
      721 aacccatttc ttcggaatgc cgatggcggc atcgagagag agctggccct taatcgaatc
      781 gtgcaccatc agcgagacgt tctcgacgtc catgacttcc tggacctggt gcatgatcat
      841 gttgaacagt cgattcacgt tcagagtgcc ggccaccgaa gaactgacct tgtacaaggt
      901 cgtcagataa tcgagtttgc ggcgcgagtc gtcgatcagg cggcgattct cgaagtgcgc
      961 ggtcagcagg cggccaatag ccaccaacag acgttggtcg tcttcgataa agggctgtcc
     1021 gtcacctttc tgatgcgccg aaagtacacc taccaggcgg tcaccggcca gcaacggcac
     1081 gcacagcacc gaccgcgaaa acgtgtcgaa gaacgaatga cgcgcgacgg tgacatcctg
     1141 cgcggcatca gccaccagca agggctggcg atgctgggcc acccagccgt gaatgccttt
     1201 tcccagggga acgcgcaggc cgtgcaactc ttcaggcagg gagtagttgc agatgctgta
     1261 gacgtcttcc ttgtcgctgt cgtagcgcat cagggccatg cccttgcacc cgagtgcggc
     1321 ggcggcatcg gccatcacgc gatcgagcag ctcggtatct tcgggttgct cggccagtcg
     1381 ctcagagatc gaatacaggc gggtcaattc gttgagcttg cgattgctgt tcgacagcag
     1441 gcgcgagttc tcgaccacct tggtaaccat cgacgccagc gtgatcaacg tcgcgcagtc
     1501 ttccttggaa aaggcgttca tctcggaaga atcgacggcg atgacgccga gcagatgatc
     1561 accgaccatc agcggcaccg ccatctcgga gtaaatggtt tcgggagtct cgatgtaacg
     1621 cggctcgagc aaaacattcg gcacgttcag cggttcgcgg ttcttggcga cccagccagt
     1681 gacgcccttg ccgagcggca cacggatctt tttcacttcc tgcgggtatt tgccgcgggc
     1741 ggcggcgact tcgagatact tcttgctttc ttttttcagc agaatcgcgc cgcaggtcgc
     1801 cccggtgata tcgatggcga tatccatgag gaactcaagc aggttattga actcaaggct
     1861 cgagttcatg atgttcgatg cttcttcgag caccgaaaga atcttctctt tgcgctgaat
     1921 cgattcacgt tcgaccgaga cttccagctg ggtggcaaag aactgctcga tcaactcgac
     1981 agccgcctgc acctgatctt ccgtcaggcg aggaacaccg gcaatctgag ctttggcatt
     2041 cgcctcgagg taggggccga gcaaaacggc accgaacaga tgatcacggg ccgtcagctt
     2101 aatcacacga atgcacgagc catccgggcg cgtcatgaag tcgcggcggg caagctgctc
     2161 aaagtcgatc gcgtccatgc gccccagcag ttcctgcgga tcgccgatca ccgcctcgcc
     2221 ccgctcattg aaaggaacga aacgaatacc ggtcagcttg ctgaaccgct gatgaaaagc
     2281 cgaaagatgc gaccgatcaa tgatttcttc gagatagaac cgtttaacca cgcattcctg
     2341 cctggtttac ccgagccctg acggtatcaa ggcacccgtg tgccagctgc atgagcccat
     2401 cgtgccgcag taaacctgaa gtaaggaaca aaaaccgcca aatgcctgtc aaaagcgttt
     2461 ggcgggtgtt ttgcccgtcc tgggagcacg cgcccccgcg aatctacgac tcaagagtgt
     2521 aacagaacga acggtattca gtcgacgtta cttgacgtca ataacggaag agcgaccgcc
     2581 gtacttgtcg gccaccgtct caccaccctt ggggtcggtg atccagtcag tgtgattgac
     2641 cacgtatttg tactcatggc gaccgggctt gagcatcaga gaggttttcc agacaccgtt
     2701 ttcgagcgtc aggggattct tgtccttgtt ccagtcgttg aagtcgccga cgatggagac
     2761 agtcttcgca gccgcatttt cataggcaaa cgccacgggt ttctctgcgc cgccagttgc
     2821 cggagccgct ggagccgcgt cagcgggctt cgccgcgtct ttcttttcgg cttccttgcg
     2881 ttcctcttct tcggcttccc actcttcctc ttcctcgaag ctgtgctcgc gaccaatgat
     2941 gtagccaatt ccaaaagcgc ttccgagaac gataagcttc cacagccaac ggaacagggt
     3001 gatcatgaac ctaacctcct ctggagtttg cgttatttat aacccgaaga ttcgcgtact
     3061 gcaagagtaa cgtttttctc cccgaccggg ggaaatcgat aatcatgcga aaatcagcca
     3121 gtgatgcgcc ttcaatcgaa agaattcttc cgcgaccgaa caaggggtga accacttcga
     3181 ccccgggtcg acagttgatc atgttggcgg tactggtctc accacgaccg cggtcggcag
     3241 ctgccggcgc ggctgtcacg gaggttggtg gttcagcgac aggctcagga acggcctgac
     3301 gcggtgcgac actttggtca ccggctgtct tcgttcgagc cgtcgaggga gcttgattat
     3361 acggagaccc ggccagcggt ggcttgtccg cgtcacgctg atagacaggg gcagtcgtcg
     3421 gcggagggta ggggagcgct tttcccgaaa acggcgctga agtgcctgtc tgtcgcgaag
     3481 ccgcggcccg cgcaagcggc gcaaaggtcg gccccttgcc acgactcaca ccaccgcgaa
     3541 cgggaacgcc aaatggcttc acctgaccag cggccggaag acgatgttcg atggatacgt
     3601 cctctccatc ctcgtcgacg cccgagctaa cgccgaacac gcgtggcatc atggttccgg
     3661 gttcgaacga ctcgcgggga atctcgccca gaaaacgcga gagtgcgttg ttctgccacg
     3721 ttccgaacat catgcgtcgc cgcgcggccg tcagatagag ccgtttcatc gcgcgggtca
     3781 tgccgacata agccagtcgc cgttcttctt cgatctcttc ctgggcgccg ctcgacgacc
     3841 ggtgaggaaa gatcccttct tccatgccga ccacgaagac gaccggaaac tcaagcccct
     3901 tggcattatg catggtcaac aaatgcacgg cgttcgccgt ttcgtcgaga tcatcgacat
     3961 cggcatgcag tgccgtctgt tcgaggtatt cccgcagtgt cagatccggt ttgagttcca
     4021 gctgctgacg aatatcagaa agcaggcttt ccacattctc tcgccgctct tccgccgtat
     4081 cagggtcgtc cttctcgaga tagtcgaggt aaccggtcaa gcgcaccacg ccacgcgcag
     4141 cttcgaacac cccctcttcg tcaagcaggg ttcgcagcct ctgcatcaac gcatgaaaca
     4201 ccgcgatctt cgattcgggc ttctgtttcg cccttgtcgc aatactctcg gcaaggactc
     4261 cttcggcttc taacgattgt actgttgctt ctccgatacc gcgtgacggc gtattgataa
     4321 tccgcttcca ggaaatggca tcgctgggat tcgcgagcac gcgcaggtag gccagtacat
     4381 ccttgatctc gcggcgagcg aaaaacttga ccccgccggt catttcgtac ggcaggcgat
     4441 tcttgaccag cgtctgctcg aaactgcggc tctggctatt catgcgaaac agaatggcga
     4501 tatcctgcaa gcgaaaacca tcgcgttcga gacgcatgat ttcgcgcacg accagatcgg
     4561 cttcttcgcg gtcgtcagaa gcgagaagaa aacgcaatcg atctccaccc cgacggtcgg
     4621 tccagagctt cttgttgcgg caactgcgat ttttcgccac cagcgcactc gcggcatcga
     4681 gaatcgcctg agtcgaacga tagttctgat cgagcacgac cacccgcgcc ccggcgaaat
     4741 ccttctcaaa ctcgagaatg ttgcgaatgg tcgcccctcg ccagccgtag atcgactgat
     4801 cgtcatcacc caccacgcac agattgcggc ttctttcgga aagcagcgcc accagcttgt
     4861 actgcgcaaa gttcgtatcc tggtattcgt ctaccagaaa gtactggtaa gttttctgca
     4921 ctttttcgag caactcaggc ttttgcgtca gcagtttcca ggattccatc aacagatcgt
     4981 caaagtcgag cgcctggtgc tcgcgcagct tctgttgata caaccgatag gccgcgccca
     5041 gaaaatctcg gtcgggtccc gatggcttgt actcatcagg cccaatgaga tcgttcttcg
     5101 cctgcgaagc caggtttaac agaaaccgcg gcgtgaactt tttcgggtca cccttgagtt
     5161 ccttgatcac cagtttcatc agacgttcct gatcgtcgtc atcataaatc gtaaagccat
     5221 ccgaaaatcc ggcatgctta tgccaccggc gcagcagcag gcaacagaat gaatgaaacg
     5281 tcgtgacggt cagacgtgaa gcatcgcccg gcatgagcgc atgcaaccgt tctcgcatct
     5341 cgcgggcggc cttgttggta aacgtgaccg cgagaatgct ctcggccggc actccgcgat
     5401 gcaagacgag attcgcgatt cggaccgtca gggtacgggt tttacccgaa cccgcgccgg
     5461 cgatcacgag gaccggacct tccgtcagtt caaccgcttc acgctggcgt tcattgagcc
     5521 cctgaatcgc ctgcatgagt gctttctctt caggagacat cgcgcatcgg cacctgttta
     5581 cccgggccgg catatgcccc aaccacccag ccgcccgcca cgaaattatt cagcccttcg
     5641 attttcaggt cgtacagagg gccctgatac tgttcagact ggacggaaac cacgatatca
     5701 tcatcgatgc cctgtcgatc gagtaccggt acgctcatgc caatcttcac gtgtgaagcg
     5761 ggcatgaggt aataaggatt ctttcgcgac agctgaatct ttttcacgat ttcaagggta
     5821 tcgagacacg ccagcgtttt cacgaacaac tgcgcttctt cgagatcttc gcggctcact
     5881 tccatgttcc ataaacccgc tttgttcacg cgacgtttga actgcttgcc ttccgcgccc
     5941 ttgagctcga cggtgccgtc aatgcgaatc agatgggaaa aaaagttgtt cgtccggcaa
     6001 cgctctccgc ttccgtacat gacaaactgg atggccgtgc tcaccgcggg gttactgcgc
     6061 gaataccgaa atgtcacgtg gggagactct tcgaacatca gcgagtcaag cagcagttgc
     6121 cgagcccgtg acggagtgtc gatgcggttg aagagttcga gtatcaaatc gtcggtcagc
     6181 tcgcacaggg cgtgtttggc cgaaaacgga aacccgggaa gaccgtattt cgtttcggca
     6241 tacttctgca gaaacagcgc tttcggcaga ctctcggtag attcgatgat ccagagccga
     6301 tcaacgattt cactgtgatt cccccagtcg ggtttggcgg tctgcatggc aaccagttct
     6361 ttcatcaact cctgcgacat gccgacccga aacccgacgg tcgagcgttc cataaggtac
     6421 agataatgat gtcggctcag cgggtttaac cgacaaaaac actgatgttc aggactcaga
     6481 cgaacctcgg cgccacgcgc ggtcttcaac gtcagcacgg ggcccttgtg atcgaactgc
     6541 acgaaaatgt cggaaacact cgtcgtcgcg gtttcgcccc aaccgcaccc gccggtgatt
     6601 ttttcacctt cgcgaatgtc cttgagcgga tgaagcaact caccgctcaa gataccgaca
     6661 tcgggtaaca acatctcgca tccctttcga tcacgttcgc gacgcgaatc gacgcgcagc
     6721 gcttgtacaa gttcgttccg gaaaacaacc ttcgtcgcgc agcatacccg aaagacgtag
     6781 aacgaggcaa ccacaagcca cgacaccagc agagtttttg tgcgtgggtt caggcttgac
     6841 gactcacccg cgccagacta ttccaggcct gtgaatcaaa ggaaagtctt tccgtgccct
     6901 gtactgacag aaggtgattg acaatcggcg ccggcctgcc taagctttca catcgtttgc
     6961 gaaagaggta tgaacatgcc gaatacgccc gtcgcccgac cgagacgtta ccagatcacg
     7021 gccatctctg atgcgctgac cgacatcatc atggaaaccc cagatgaggc actggccggg
     7081 ttagggcttc ccaagggcaa cagcatcgag ttgaactcgg aaaacctgca gaagctcgag
     7141 agtcttctgc tcaagcatga gccaaccatc tcgcccggcg gttctcccac caacgtgatc
     7201 tacggcgcgg cgaatctcgg cctgcattgt gccttcatcg gttgcgtagg cagcgatgac
     7261 tacggttacg attacatcaa caacctgcgg gaaaacggca tcgataccta tgtttcgatc
     7321 aagaaaggga aatcgggcgt ctgctacacc ctgatcaccc ccgatggaca gcgcaccttc
     7381 ggtcttgatt tcggggtcac caagatgctg catgattacg agattctgca ttcactcgtt
     7441 cgagattcgc agtatctcca tttttctgct tatgagttgc gtggcgacag tccgatcaac
     7501 aaagcgacca gacacgccta ccagatcgcc cgcaaacatg gcacgcgcat ttcgttcgat
     7561 ctgggcgaca gtttcgtgat cagcaagatt cgcgacaagc tgttcgagtt tctcaatgac
     7621 aaggtcgaca ttcttttcgc caacgaggat gaagcggtct gcctgagcca gagtcgcgac
     7681 ttccgacgtc tgctggcctt ttcggatctc gtcgtgatca aacttggcga acgtggctca
     7741 ctggcactca cccctgacaa cgaggtggcc attcccgcct acaaggtcga tcaggtgaaa
     7801 gacaccaatg gggcgggcga caactttcag gcgggtttct tctacgggtt gttcaagggc
     7861 ctgcctctcg aaacctgcct gaaaatcggc aactttctcg cagccagcgt cattcgacgt
     7921 gtcggagcgc aggccaaagt gaagatctca ggcatcgagt ttttcattta atcgcctcca
     7981 gatagaaacc ttcccagggt catttagttc tcccagattg atttgtctgc aatggctttg
     8041 aatgttcgtc tcttcacgtc tccgaaccac tctatgcctt gcaagttgtt gttgaacccc
     8101 agtttgctga tcagaaggat cgcgagattc cgcatggtag ccaggaattg ggcaattgcg
     8161 ggagttcggc aac
//
LOCUS       DAORHD010000033         8080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8477_length_8080_cov_3.806854, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000033 DAORHD010000000
VERSION     DAORHD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8477_length_8080_cov_3.806854"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2599)
                     /locus_tag="PKO06_01405"
     CDS             complement(<1..2599)
                     /locus_tag="PKO06_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68324.1"
                     /translation="MRYRGTWSPVVLLFLCLLLGARHATASSPDSYEPNGKREEAPFI
                     ATETPLFATLFPVGDQDYYRVPIATGLPLQLDIALDDVASNFNPHLTLFDAKGTEIFR
                     HEVAPGQPMRCRVNLADPGSLTIRLVDSCHGIGEVGYSHEWNNQAATQPYRLSVQATP
                     MPDSAEPNNTAETAFPLSLNQDFRGRLFPARDEDWYKVDIPAGKRGLLKVVFSHPSDL
                     IQPHALLRNSSGQDIDASSVSRGEQAVLLAEVSSGTYRFRFIDGNQGKHEVGYSHEWG
                     NQASTQEYQGSVQFLEVVDQYEPSSKDEPATFTLNTEVSASLFPRGETDFYAFEIPAP
                     GVGVLQIEADCSLAPVVPVLRLLKSSGEEVTRATGAEGGLAKLTTHLRSPGRFIVELF
                     DSNTGTWEVGYSHETANRTGPYKLRINFTPIIDSDDPHQSYETALELPQGTSVSSTLF
                     PAGEQEYFRCTVPGSGPTAFEFSIPDLDFPIAPQCVRMDAPGQDTQHFRVGVYQPISE
                     RLILQAPGSYTFRVLDGNHGLHEVGYSHEIANQCSRQPYHVFWRVSPPRPEPLASSVI
                     PLKEHDTATAAVAVAMPASCSFFLYPAGDQEWLLLPQTGPGLLEGRFDATDQRLNPRV
                     AVFTSATDTSKPFSEVDNSYSSIEHFRVRLPFRSNWLLRVTAATGSSSNPIHAQLWMH
                     DLNETGPEAAGIPLEVANLQQGLSGRIFPAGDSDAFQLTLPSAGTLHVSGFEPDGISL
                     NTTFSAIASPALRVLYLVGGRTDYSLFNQTSGQVVAKRLQEESLEYLRHFPFYSTYAR
                     GDCPPPIGATQSFHLQIPAGMQAGRLYVTAFGMDPNVVRFTCNQQPLKGTRISAWGQW
                     AEVAYEIASL"
     gene            complement(2596..5571)
                     /locus_tag="PKO06_01410"
     CDS             complement(2596..5571)
                     /locus_tag="PKO06_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV68325.1"
                     /translation="MKKIPCFLALVAFSAMLPLSLFSADLTLIAAPNGRAGILASATS
                     GIRSQYLAETLRLSEIRANDFPLRMTTPRSGEGDIFGIGEEFSIRCDLPADVSYHINL
                     FSLGTRGNLVRLFPNKWAPDSLVSGSSSVVIPPPKARFALRTSEPPGTDLILAIAIRG
                     DDATMSQQKTRAAGPFALFDDLASAATVISDWTRAEKGKQVSVRHLAIHTRGAEPGDG
                     AFSVGRSGDWVARLWIDQSRLQAGQPVFLKVLSNKSATISTVSFKPTNGSPSSIIPND
                     SSIDCNDHELLVLPRAEDSWAWQAQGPSGDGVLQLVLAGGDAASITLDLPLSIEGEFL
                     PPASPAVSSVTAPMTASATPITVTATASVPATTSDQMSSTGATTPTQPPVLVTASTTQ
                     TPSTESTTPDIATPSSSETVVTAPATEDLPRPVPASRTVSISAFLAAARQGKVRDVQA
                     LLQRFPWLVEARGERRATALHWAASEGHAEIVSLLASAGAWVNAPADGDMRPLHLAAW
                     HGHTETVRRLIAAGAGVNPVNRFHETPLLLAVKERRRETVQELLRHGALPEIADNNGS
                     TALNWAGVNQDTALIASLEAAGCRTSAARTLPLVHTHAAAGNLEAVKADLASEAASLH
                     AVDQRGRTPLHIAAEHRFAEIVKYLLTAGARVDAVDFSGRTALHLACQRADQLIVSQL
                     LEAGASLTTPDADQRTPIHLLCLSDVDESDLLTLLKLIPIANQAFRARDVDGCLPIHL
                     AVQKRQRSVYSYLCAIDLPAELRKTGEGLSCLGLAVRHAESDTIDAFIPSTADLNSRE
                     LGRSFTLPLVAAEAGNTGMLRALLERTFSQSPALDGTTLLHAAARSGNAEAVSIALRH
                     HPMLEAVDVEGNTPLHLATSLAHADTAALLIASGASVQTANGDGMTALHLAALSGSTD
                     VLAAVLKKGVSVQTPTSTGDLALHLAAEAGNTEIVERLLAAGARRDTRDARGQTAAGR
                     ARAAGFDDIADYLERETRP"
     gene            complement(5639..6979)
                     /locus_tag="PKO06_01415"
     CDS             complement(5639..6979)
                     /locus_tag="PKO06_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HNV68326.1"
                     /translation="MVPDHSSTLPRPMPLHTRICLGLLAGGACGVGANVLAGEHAALH
                     WLITRVTEPVGQIFLRLMVMVVVPLVFSSLTLGVAGLGNLRQLGRIGLKTLALFLLST
                     AIAAGCGLLLVNTIRPGVGLADEVKTRLIEQYGTQSRESLAGVGTAKPAALGLGALLE
                     FIPRNPLAAAVQGDMLALIVFSLLVGIALTGMPKERAQPLLGVLTALADAMVAIIDLT
                     MRLAPFGIAALIFSVTARFGIDLLYKLGWYVFTVVTGLFVHQFVVFSLALLLLARLSP
                     WRFFTAIESIILTAFSSSSSNATLPTTMRIAEEEVGIPREICGFVLPLGATMNMNGTA
                     LFEGVTVLFLAQVFGVSLSLTTQLLVVWFCVLSAIGTAGVPGGTIPLLIMVLQSVQVP
                     GEGIALIMGVDRLLDMCRTTVNVTGDVVVAAIVTRSENLPFRPYQAPPLPTAPE"
     gene            complement(6969..7913)
                     /locus_tag="PKO06_01420"
     CDS             complement(6969..7913)
                     /locus_tag="PKO06_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNV68327.1"
                     /translation="MAKTGILLESGTNELEIAEFYLDKQSYGINVAKIREIVLFDPDK
                     VTRFAGAQNGVLGTILFRGHSIVLVNLPQLLNLQPPPEGTRQVVLVSEFNQRTTAFLV
                     DGVARIHRISWAEFTPIHPLLGTFTSYTTGSFHIDKREILILDFESILNDLFPETRMH
                     EQPEAEKSLADRRANLRVFIVEDSRTIQALIISRLKGAGVTRIKTFSNGRLGLEALQQ
                     IAERKASGAASSDDDVDLVVSDIEMPELDGLSFCKQMKNTPGLARPIIMFSSLITEEM
                     ARKCRDCGADRWVSKPELDKVIGFADELCLGASPSHGS"
BASE COUNT         1725 a         2230 c         2546 g         1579 t
ORIGIN      
        1 ttaatgaagc gatctcatag gctacttcgg cccactgccc ccaggcggag atccgcgttc
       61 ctttcaaggg ttgctggttg caggtaaagc gaaccacatt cggatccata ccgaatgccg
      121 tcacatacag acgaccagct tgcattccgg caggaatctg caggtgaaag ctctgggtgg
      181 caccaatcgg aggtgggcaa tcaccacgcg cataggttga atagaaggga aaatgtcgga
      241 gatactcgag agactcttcc tgcaagcgtt tggccacgac ctggccagac gtttgattga
      301 acagggagta gtcggtacga ccgccgacga gatacagaac ccggagagcc ggtgatgcaa
      361 tggctgagaa tgtcgtgttc aggctgatcc cgtccggttc gaacccggac acgtgcagtg
      421 tgccggctga cggcagtgtg agctgaaatg cgtcactgtc tccggctggg aagatgcgac
      481 cggaaagtcc ctgctgcaga ttcgcgactt ccagaggaat tccggcggct tccggtccgg
      541 tttcgttgag gtcgtgcatc cacagttgag cgtggatcgg attgcttgac gaccctgtgg
      601 ccgcggtgac gcgcagaagc cagttgctgc gaaacggcag tcgaactcga aaatgttcga
      661 tgctggagta cgagttgtcg acttctgaaa acggcttgct ggtatctgtt gccgaggtga
      721 atactgcgac gcgcgggttc aggcgctggt cggtggcgtc gaaccgccct tccagcaggc
      781 cgggaccggt ttgtggcaac agcagccatt cctgatcgcc cgcgggatac agaaaaaagg
      841 agcagctggc gggcatggca accgccacgg cggccgtggc ggtgtcgtgc tctttcagtg
      901 gaattaccga actggccaga ggttcggggc gtgggggaga tacgcgccag aacacgtgat
      961 agggctgcct cgagcactgg ttcgcgattt cgtggctgta gcccacctca tggagaccat
     1021 gatttccatc cagaacccga aacgtgtatg agccgggagc ctggaggatc aaccgttcgg
     1081 aaatcggttg atacacgccg actctgaaat gctgtgtgtc ctggcctggg gcatccatcc
     1141 ggacacactg cggggcgatc gggaaatcca ggtcgggaat gctgaactcg aaagcggtcg
     1201 gaccgctacc gggcaccgta cagcgaaagt attcctgttc gccggccgga aagagagtcg
     1261 atgaaacgct ggtgccctga ggcagttcga gagccgtttc gtaagattgg tgtggatcgt
     1321 ccgagtcgat gatcggagta aagtttatgc gaagcttata gggaccggta cggtttgctg
     1381 tctcgtgaga atagcctact tcccaggttc cagtgtttga atcaaacagt tcgacaataa
     1441 accgacccgg cgaacggaga tgcgtcgtga gcttcgccag tcctccttcg gcccccgtcg
     1501 ctcgggtcac ttcttcgcct gacgatttca agaggcgaag caccggcacg acgggcgcca
     1561 gcgaacagtc ggcttcgatc tgcagcacgc cgaccccggg tgccggaatc tcaaacgcgt
     1621 agaagtccgt ctctccgcgg ggaaaaagcg atgctgagac ttcggtgttc agcgtaaagg
     1681 tcgccggttc gtccttactc gagggctcat actggtcgac cacctccaga aactgcaccg
     1741 atccctgata ctcttgagtc gatgcctgat ttccccactc atgcgagtat ccgacttcgt
     1801 gttttccctg gttcccatct atgaagcgga accgataggt ccccgagctc acctcggcga
     1861 gcagaacggc ctgctcaccc cgtgacacgc tcgaggcgtc tatgtcttgc cccgaactgt
     1921 tgcgaagcag ggcatgtggt tgaatgagat cgctgggatg cgaaaacacg accttgagca
     1981 ggccacgttt tcccgctgga atgtcgacct tataccagtc ttcatcgcgc gctggaaaga
     2041 gacggccgcg aaagtcctgg ttcaaggaga gcgggaatgc cgtttccgcg gtattgtttg
     2101 gctctgccga gtcgggcatc ggggtggctt ggacggacag gcggtacggc tgcgtggcgg
     2161 cctggttgtt ccactcatga gagtagccga cttccccaat gccgtggcag ctgtcgacca
     2221 gtcggatcgt caggctgccg gggtcagcca gattgactcg gcaacgcatg ggttggccgg
     2281 gggcaacctc gtgccggaag atttctgtac ccttggcatc gaataacgtc agatgcggat
     2341 tgaagttcga agcgacatcg tcgagagcga tgtcgagctg cagaggcaac ccggtcgcga
     2401 tgggtactcg ataatagtcc tggtcaccaa cgggaaaaag cgtggcaaat agaggagtct
     2461 ccgttgcaat gaacggagct tcttctctct tgccgttcgg ttcatacgag tcaggggagc
     2521 ttgcggtcgc atgtcttgcg ccaagaagca gacacaggaa gaggagaaca acgggtgacc
     2581 atgttccgcg atatctcatg gtcgtgtctc ccgctcgagg tagtcggcga tgtcgtcgaa
     2641 gccggcagcg cgagcgcgcc cggccgccgt ctgaccgcgg gcatcccggg tatcacgtcg
     2701 agctccggcg gcaagaaggc gctccacgat ctccgtatta ccggcttcag cagccagatg
     2761 cagcgcaaga tcacctgtac tcgtcggtgt ctgtaccgag acacctttct tcaataccgc
     2821 cgccaatacg tcggttgaac cactcagcgc cgccagatgg agcgccgtca tcccgtctcc
     2881 gttcgcggtc tgcacgctcg cgccagacgc aatcaacagg gctgccgtgt ctgcgtgtgc
     2941 cagagaggtc gccaggtgca ggggggtgtt gccctcgaca tccaccgctt ccagcatcgg
     3001 atgatgtcga agtgcgatcg ataccgcctc ggcattgcct gatcgtgccg ccgcgtggag
     3061 cagggtcgtg ccatcgagcg cgggcgattg cgaaaacgtg cgctcgagaa gagcccgtag
     3121 catgcctgtg tttccggctt cagccgcaac cagaggcaat gtaaacgaac gtccgagttc
     3181 gcgactgttt aaatctgcag ttgatgggat aaaggcatct atcgtatctg attccgcatg
     3241 ccggaccgcc aggcccagac aggacaagcc ttcgccggtc ttgcgcagct cggcggggag
     3301 atcgatggcg cagagatacg aatacacgga tcgctgccgc ttttgcacgg ccaggtgtat
     3361 cggcaggcac ccgtccacat cacgcgcgcg gaatgcctgg ttggcgatcg gaatcagttt
     3421 cagcagggtc agaaggtctg attcatcgac atcagaaagg cacagcaggt gaatgggggt
     3481 tcgctggtcg gcatcgggag ttgtcagtga ggcacccgcc tcgagcagtt gtgacacgat
     3541 gagctgatcc gcgcgttggc aggccagatg cagggcagta cgtccggaaa aatcgaccgc
     3601 atcgacgcga gcgccggctg tgagaagata cttcacgatt tccgcaaagc gatgctctgc
     3661 ggcgatgtgc aggggcgtgc gcccccgttg atcgacggcg tgcagagaag ccgcctccga
     3721 agcaagatct gcctttaccg cttcgagatt cccggccgct gcgtgggtgt gaacgagcgg
     3781 gagagttcgg gccgccgacg tgcggcaacc ggcggcttca aggcttgcga tcaacgcggt
     3841 gtcctgattc actcccgccc aattgagcgc ggtgctgcca ttgttgtccg cgatctccgg
     3901 cagagctccg tggcgcagga gttcctgtac tgtttcccgg cggcgttctt tcaccgcgag
     3961 caacagcggg gtttcgtgga aacggttgac gggattgact ccggccccgg cggcaatgag
     4021 gcgtcgaacg gtttcggtgt gcccatgcca ggctgccagg tgcagcggac gcatgtcacc
     4081 gtccgcgggc gcattcaccc aggctccggc agatgccagc agcgagacga tctccgcatg
     4141 tccttcgctg gcagcccagt gcagagctgt cgctcgacgt tcccctcggg cttcgaccag
     4201 ccagggaaaa cgctgcagta gcgcctgcac gtcgcgcacc ttgccctgac gggccgccgc
     4261 aagaaacgcc gagatcgaga cggttcgcga agcgggcacc ggccgcggca agtcttctgt
     4321 ggctggcgcg gtgaccaccg tctccgaaga tgacggagtg gcgatgtctg gcgttgttga
     4381 ttcggtcgaa ggcgtctgtg ttgtcgaggc ggtgaccagc actggtggct gagttggcgt
     4441 tgtggcaccg gttgaagaca tctggtcaga ggtggtcgct ggaacggatg ccgtcgccgt
     4501 gaccgtaatc ggggttgccg aggcggtcat cggtgcggtg acagatgaaa cggcaggcga
     4561 agcaggtggc aggaactctc cttcgatgct cagcggcaaa tcaagagtga tcgatgccgc
     4621 atcaccgccg gcgagcacga gttgcagaac cccgtctccg gaaggacctt gggcctgcca
     4681 ggcccacgaa tcttcggcgc gcggaagtac gagtagctcg tgatcgttgc aatctattga
     4741 agagtcattg gggatgatgg aagatggaga gccgttcgtt ggtttgaacg acacggtcga
     4801 aatcgtcgcc gatttgttgc tgagaacctt gagaaatact ggctgtccag cctgcagacg
     4861 cgactggtcg atccagagtc gggcgaccca gtcgcccgat cgaccgacgg aaaatgcgcc
     4921 gtcaccgggt tcggctccgc gcgtgtgaat ggccagatgt cgtacgctga cctgtttgcc
     4981 tttttccgcg cgcgtccagt cgctgatgac tgtcgcggct gaggcgagat catcgaacag
     5041 agcgaaagga ccggccgctc gggtcttctg ttggctcatg gtggcgtcat cgccgcgaat
     5101 ggcaatggcg agaatcagat cggtgccggg gggctcgctg gtacgcaggg caaatcgggc
     5161 tttcggcgga ggaatgacca cggaactgga tccgctgacc agcgagtccg gagcccattt
     5221 gttggggaaa aggcgaacca ggtttccccg ggtcccaagg ctgaagagat tgatatggta
     5281 ggagacatca gccggcagat cgcagcgaat ggaaaattcc tcgccgatac cgaaaatgtc
     5341 gccttcgccg gagcgcggtg tcgtcatgcg cagcgggaag tcgttcgcgc gtatttccga
     5401 aaggcgcagt gtttctgcca gatactgact ccggatcccg gacgtggccg acgccagaat
     5461 tcccgctcgg ccgttcggag ctgcgatgag cgtcagatcg gctgagaaaa gggaaagggg
     5521 aagcatggcg ctgaacgcaa ccagcgcgag gaaacacggg atcttcttca tacggtgctc
     5581 ctgaatccgg atctgccgca gatggcagaa agatgaacgt gactgacgaa caagaacttc
     5641 attccggtgc cgttggcagg ggaggtgcct gatacggccg aaatggcagg ttttcagatc
     5701 gagtgacgat ggcggcaacg acaacatcac ctgtgacgtt gaccgtggtg cggcacatat
     5761 cgagaagccg atcgacgccc atgatcagtg caattccctc gccgggaacc tggacgctct
     5821 gtaaaaccat aatcagcagt ggaatggttc cgccgggcac gccggcagtg ccgatggccg
     5881 aaagaacgca gaaccagacg accagcagct gagttgtcag gctcaacgac accccgaaca
     5941 cctgagcgag aaacagcacg gtgacgcctt cgaagagggc agtaccattc atgttcatcg
     6001 tggcgccgag tggaagcacg aaaccacaga tttctcgggg gataccgact tcttcttccg
     6061 cgatgcgcat ggtcgttggc agagtggcgt tcgaagaact cgaagagaag gcggtcagaa
     6121 tgatcgactc gatggcggtg aaaaaacgcc agggagaaag gcgggccaaa agcagcagcg
     6181 cgagagagaa aaccacaaac tgatgcacga acaggccggt gacgacggta aagacatacc
     6241 aaccaagctt gtacaaaaga tcgatgccaa agcgcgccgt cacgctgaag atcagggcgg
     6301 ctatcccaaa cggagccaga cgcatggtca ggtcaatgat ggcaaccatg gcatcggcaa
     6361 gagctgtcag aacgccgagc aggggttgtg cgcgctcctt gggcatgccg gtcaacgcga
     6421 tgccgaccag aagtgagaaa acgatcaggg ccagcatgtc accctgcacg gcagcagcca
     6481 gcgggtttct cggtatgaac tccaggagtg ctccgagccc caaagcggct ggcttcgccg
     6541 tgccaacgcc ggccaacgat tcgcgtgatt gcgtgccgta ttgctcaatc aaccgcgtct
     6601 tcacctcgtc ggcgagaccg acgcccggtc gaatcgtgtt taccagcaac aggccgcagc
     6661 cggcggcaat cgccgttgac agcaggaaca gggccagtgt cttcagaccg atccgtccga
     6721 gttgacgcag attcccaagc ccggctactc caagggtcag cgacgagaat accagtggca
     6781 cgaccaccat gaccatgagc cgcaaaaaaa tctggcctac cggctctgtc actcgcgtga
     6841 tcagccagtg cagcgcagcg tgttcccccg ccagcacatt cgcgccgacg ccacatgcac
     6901 caccggcgag aaggccgaga cagattcgag tgtgcaaggg catggggcgc ggcagtgtgg
     6961 aggagtggtc aggaaccatg gctgggtgaa gcacccaggc agagttcgtc ggcaaagcca
     7021 atgaccttgt cgagttcggg tttcgaaacc cagcggtccg cgccacagtc tctgcatttt
     7081 cgcgccatct cttcggtgat gagtgaggag aacatgatga tcggacgagc cagtccgggc
     7141 gtgttcttca tttgtttgca gaacgacaat ccgtcgagtt cgggcatttc gatgtctgag
     7201 acgaccagat cgacatcgtc atcggaggaa gccgcacccg aagccttgcg ttcggcgatc
     7261 tgctgcaggg cttccagccc gagacgaccg ttgctgaagg ttttgatacg cgtgactccg
     7321 gcgcccttga gacggctgat gatcagtgct tgaatggtgc gagaatcctc gacgataaag
     7381 actcgcagat tggcgcgtcg gtctgccaga ctcttttctg cttcgggttg ttcgtgcatg
     7441 cgggtctcag ggaacaggtc gttcagaatc gactcgaagt cgagaatcag aatctcgcgt
     7501 ttgtcgatat ggaagctgcc ggtggtgtag gacgtgaagg ttcccagcag aggatgaatc
     7561 ggggtgaatt ccgcccacga aatgcgatga attctggcca cgccgtcgac gagaaatgcg
     7621 gtggtgcgct gattgaactc cgagaccaga accacttgac gcgtgccttc gggcggtggc
     7681 tgcagattca gcaactgtgg cagattcaca agaacgatcg agtgaccgcg aaagagtatg
     7741 gttcctagaa cgccgttttg agccccggcg aaacgagtga ctttgtcagg gtcgaacagg
     7801 acgatttcgc gaatcttggc gacattgatt ccgtaactct gtttgtcgag ataaaactcg
     7861 gcgatttcca gttcattggt accactttca agcagaatgc cagtcttggc catcgggcgt
     7921 tcctccctct tcgagatatg tataaatcct caagagtata cggcaactcg aagagaaaat
     7981 ctcgtcctaa aaactgcttt aaaaagtgta cggcattgcg atatgcttgt ggaaagacac
     8041 tcgtgtcggt ggaggctcta tgcgcgcttg gttcttcgcc
//
LOCUS       DAORHD010000034         8045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8534_length_8045_cov_4.263830, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000034 DAORHD010000000
VERSION     DAORHD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8534_length_8045_cov_4.263830"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="PKO06_01425"
     CDS             <1..888
                     /locus_tag="PKO06_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68328.1"
                     /translation="AVFALPILGLVVAEAHEAEALARLQGTQFDLFALKNLQTLDCTQ
                     QRRTDSPLLRVWNADLDLELVPVETVLNGSWQRFLEGMNQKQGEYVLTLVRAEAMQPL
                     LVIPLRGGFDTSGTLTAACEALSLRAEDAVIQLRKLLETAPDTQDVRYHLGRAYETLA
                     LQGKKPREHSALTRQLFDEELALNPSNVKAIAGIGLWHKRAGQWAEAEHWLRRALDLA
                     PTNIPILLSLATTVFNRVGVGPKGTPVSEEAFGLLAQAWDLSPRHPEVRTLAIEWGRL
                     FRRDLLREFRGRPARISWP"
     gene            complement(966..3110)
                     /locus_tag="PKO06_01430"
     CDS             complement(966..3110)
                     /locus_tag="PKO06_01430"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV68329.1"
                     /translation="MIDTFFSLDALGSPIALFISLLIGLGFGLSLEQAGFGSSRRLAG
                     IFYFRDMTVLKVMFTAVLTAMLGLSWLLAAGLIKLDSINLLETIYGAQIIGGLLLGIG
                     FVMGGWCPGTAAVGLASGSLDALLFLFGAMGGAVIFNEWYPRLAEYVNWGNQGVLFVY
                     DTLGTTRPVFALAFTAVGVAAFWGSEFLEEYVGTGNLRRHAPVLVVVSIGLLLSAGGL
                     FVTPASPTPGSGHVASFAQVRPGSASISSSDQALLETIETAADHIEPEELADRLMAGD
                     AGLYLVDVRSPAEYAAYHLPGAVNLSLPDLPTALAARRNQGTIVLYSNGMTHPAQARD
                     VLQRQGYQNVYLLTEGLVGFLDRCLKPASVRATVLSDAELKRINAWRSFFLQAAPLPA
                     AASQRGREPDRPSSEGQAVIWPSAVKPEVALPGLVSTAWLADNLGKPGVKVLDLRPQP
                     EYNRGHLPGAVSLQVESLRGMSAGVPSMLLPSSMIVQHFSQLGLRADDIVILIHGEKL
                     HDATLVGMAFDRIQHRYWGILDGGHALWLAEKRPLTRDLPQVQPVSRPPVEESDRFTV
                     TSEQVLRSVRQQDSVIVDVRPVVNFLGTESDEARAGHIPGAVNRPYTEEVVTSSDGVV
                     RFRSRQDLASAWSALGVRPRSENPSPNDGRSTEKPVIVHCRTGHQASQAYFLLNYILG
                     FRDILWYDGGWTEWASRPDLPLATGTPSESGN"
     gene            complement(3103..3642)
                     /locus_tag="PKO06_01435"
     CDS             complement(3103..3642)
                     /locus_tag="PKO06_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNV68330.1"
                     /translation="MDAPESKSAGNTSPQPYLNPYLAGLLLGLTLLASFLILGAGLGA
                     SAGMARLAAFCQMCIAPEHVLSSEYFGGWGKSPLNYYLVFMLAGTFLGGFLSAWLSGR
                     QQVRLERGTACAGATRAVLAIVGGVIAGFASRLAQGCTSGQALTGSALLLTGSLVYLC
                     CVFAGGYAAAWFFRSQWHD"
     gene            complement(3646..5691)
                     /locus_tag="PKO06_01440"
     CDS             complement(3646..5691)
                     /locus_tag="PKO06_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV68331.1"
                     /translation="MQSFSLWRRLVLIVALCWSLALHAGTIKDTESASHDDTAAKYDL
                     VATYDGGAYRILQYNLAVLSHYSYALISGKEALIVDPGRDIDTYLELLKKEGLTCRGV
                     FLTHSHADFVAGHTELAKALGCKMYASHVSGAEYPFEPLRDGTTLEVGDALLSFLETP
                     GHTPDGICALVAAKSSPKEPKHLFTGDTLFVGSVGRPDLMGGTTSASALAGMLFDTWT
                     NKLSKLPDSVSIFPAHGAGSLCGAHLSDEPTSTLGAQKQVNSYLQYAGSRSAFIAAVL
                     DGLPEAPQYFKHNAQMNRKGPALVDWAATPEVVAPQAALTDVSRGYVVDVRDAKTYAD
                     GHIPNSVNIGVRGRLETWVGIMVPWGSNLILCGSPAETAESVRRLHRVGYTAKTIDWT
                     AWKTAGLPVARNQMTTPEALYQGMQKGDAPLIVDVRLPTEWMGLRIGQVLNLPLNHLG
                     ETAPVKLEKEQPVVAVCNSAYRSSMAVGVLERLGFSKASSLEGGSEAWIAAGYPVFGA
                     TAAGSHATPAPTQLRSVNLPDRLAPADLRRLKLDLPGTFEIIDLRPAAAIADYNPLQA
                     RPVDVAAALSDPSLLVGDIPLIITDRDGSLAMMIAGILSQKTKRPIKALHGGMESWWT
                     DNEKQRLLTPSSTGTTAPTGVAPRISPPIVAPITTPAAPSSPAPAQPAPKKKKSAGC"
     gene            complement(5773..6507)
                     /locus_tag="PKO06_01445"
     CDS             complement(5773..6507)
                     /locus_tag="PKO06_01445"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNV68332.1"
                     /translation="MALLVPGLGFGAEGQIPLIGEPAPAFTADTTQGKINFPADYKGK
                     WVILFSHPADFTPVCTTEFMTFAKMYKQFKALNCELVGLSIDSMYSHIAWLRTIKEKI
                     HYRGMKNVEVTFPLIEDIKMNVAKLYGMVHPGAADTKAVRAVFYIDPTGKIRAMMYYP
                     LEAGRNFAELKRLLIALQTTDAHKVACPADWQPGDDVIVPPPGSCGAAKDRVQKTEGV
                     HCYDWFFCTKKLPRSQLKLGAQTDEE"
     gene            6959..>8045
                     /locus_tag="PKO06_01450"
     CDS             6959..>8045
                     /locus_tag="PKO06_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV68333.1"
                     /translation="MFRDGPGEEQDQRQYANRELIRRLIPFVRPHLAQMLIAALMMGV
                     TVALGLAGPLLIRRGIDRDIAGRDLEGLKWTAFLFIVVQLGTILVYYFMAVRLEKVGQ
                     AVMARLKKSLFAHLLGLSVDYFDVNPVGRLLSRVESDTNTLRRLLSHTALQVASNLLM
                     LLGMLGVMFWADARLALATMLMLPILVGLLYWFQTKVRARFKRVRKLAAEVSAFVAER
                     VGGIRLIQAFGIEKQIQSRLATVNAVKFEAAYHAEQAVIMFFNGIQMVQVLGLALVLG
                     LGGYWVTAGSMSIGTLVLFLNYIQRFFGPLMALSEELNVTQRAFASADRIFGILDTPS
                     RVAEPTEAVSVPKAFQTLEFREVWFAYK"
BASE COUNT         1609 a         2292 c         2602 g         1542 t
ORIGIN      
        1 gcggttttcg cgcttccgat actgggtctg gtggtcgctg aagcgcatga ggctgaagcc
       61 ctggcccggc tgcagggcac gcagttcgat ctcttcgcgc tgaagaacct gcagactctt
      121 gactgtacac aacaacgccg cacggattcg ccattgctgc gtgtctggaa tgccgatctc
      181 gatctcgagt tggtaccggt tgaaacggtg ctgaacggtt catggcagag gttcttggaa
      241 ggaatgaacc agaaacaggg agagtatgtg ctgacgcttg tccgggctga agccatgcag
      301 cctttgctgg tgatcccatt gcgcggtgga ttcgatacct cgggtaccct gacagccgcc
      361 tgcgaagctc tgtcgttgcg ggccgaagat gcggtgatcc aactgcgtaa acttttagag
      421 accgcgccgg acactcagga cgttcgctat catctcggac gtgcgtatga aacgctggcc
      481 ctgcaaggga agaagccgcg cgaacattcg gctctgactc ggcaactgtt cgacgaagag
      541 ctggccctga atccgtcaaa tgtaaaagcg attgccggta tcgggctgtg gcataagcga
      601 gcaggccagt gggcagaggc ggaacactgg ctcagacgag cgctcgatct cgctccgacc
      661 aacattccga ttctgttgag tctggccacc acggtgttca accgcgtggg cgtcggaccg
      721 aagggcacgc cggtgtccga agaagcgttc ggcttgcttg cccaagcctg ggatctcagc
      781 ccccggcacc ccgaggttcg cacgctggcg atcgaatggg gccgcctttt ccgccgcgat
      841 ctgcttcgtg agttccgagg ccgccccgcg cggatcagtt ggccgtaaga cggcagttgt
      901 ggtcggatcc gcaacagaaa gcccgccagt ccgggatcgg aatggcgggc ttcgtggaat
      961 cggggctaat tgccgctttc cgatggcgta ccggtggcca gaggcagatc ggggcgggat
     1021 gcccactcgg tccaccctcc gtcgtaccag aggatgtcgc ggaacccgag gatgtagttc
     1081 aacagaaagt atgcctgact ggcctgatga ccggtgcggc agtgaacaat gacgggcttc
     1141 tcggttgaac gaccatcatt cggtgatggg ttctcgctgc gcgggcgaac acccagagcg
     1201 ctccaggcgg aggccaggtc ttgacgactc ctgaaccgga cgacgccatc agaggaggtg
     1261 accacctctt ccgtgtacgg tcggttcacg gcgccgggaa tgtggccagc gcgcgcttcg
     1321 tccgattcgg tgccgagaaa gttcaccacc ggccggacat cgacgatcac cgagtcctgt
     1381 tgacgcacgg atctcaacac ctgctcagac gtcaccgtga agcgatcact ctcctcaacg
     1441 ggcggtcgag acacaggctg cacctgcggc aggtcgcggg tcagcggccg tttttccgca
     1501 agccacaggg catgaccgcc atcgagtatc ccccagtacc ggtgctggat ccggtcgaag
     1561 gccattccga ccagggtggc gtcgtggagt ttctcgccgt gaatcaggat gacgatgtcg
     1621 tccgctctga gtcccagttg agaaaagtgt tgaacgatca ttgaagaagg aagcagcatg
     1681 gaaggaactc cagccgacat gccgcgcaag ctttcgacct gcaaggagac ggcgcctggg
     1741 agatgtccgc ggttgtactc gggttgcggt cgtagatcga gaaccttgac tccgggcttc
     1801 cccaggttgt ccgccagcca tgcggtcgag accaggccgg gaagcgcgac ttcgggtttc
     1861 acggcagaag gccagatcac ggcctggcct tcactcgaag ggcgatctgg ctcgcgacct
     1921 ctctggctgg cagcggcagg gagaggcgca gcctgcagga agaacgatct ccatgcgttt
     1981 atgcgtttca gttcagcgtc tgaaagaacg gtcgctcgga cggatgccgg cttgagacag
     2041 cggtcgagga acccgacaag accttcggtc agcaggtaga cgttctgata cccctgacgc
     2101 tgcaggacat cacgtgcctg agccggatgc gtcataccgt tcgaatacag aacgatggtt
     2161 ccctggttgc gtctcgcggc cagagcggtt ggcaagtcgg gcagcgagag attcacggcg
     2221 ccgggaaggt ggtacgcggc gtattccgcc ggtgaacgga catcgacgag atacagcccg
     2281 gcatcgcctg ccatcaggcg atcggccagt tcctcgggct cgatgtggtc ggccgcggtt
     2341 tcaatcgttt cgagaagagc ctgatcagat gatgagatcg acgcagaacc cggacgaacc
     2401 tgagcgaacg aggcgacgtg gccgctgccc ggcgtcggtg aagcgggcgt cacgaacagt
     2461 cctccggcag aaagcaggag accgatcgaa acgacgacga gtacgggggc gtgacggcga
     2521 aggtttcccg tgccgacgta ctcttcgaga aactccgaac cccagaacgc cgcgacaccg
     2581 acggcggtga acgccagcgc gaagaccggg cgcgtggtgc cgagcgtgtc gtagacaaag
     2641 aggactccct gattgcccca gttcacatac tcggccaggc ggggatacca ttcgttgaag
     2701 atcaccgctc cgcccatggc gccgaacaga aacagcagag cgtccaggct gccgctcgcc
     2761 aacccgaccg ctgccgtgcc cgggcaccag ccgcccatga cgaacccgat accgagaagc
     2821 aggccgccga tgatctgcgc gccgtagatc gtttcgagca gattgatgct gtcgagtttg
     2881 atcaacccgg cggccaagag ccaggaaaga ccgagcatcg ccgtgagaac ggcggtaaac
     2941 atcactttga gcacggtcat gtcgcggaag tagaagatgc cggccaggcg ccgcgagctg
     3001 ccgaatccgg cctgctcgag agagaggccg aacccgagac cgatcagaag cgagatgaag
     3061 agagcaatgg gacttccgag ggcatcgaga ctgaagaagg tatcaatcat gccactgact
     3121 cctgaagaac caggccgcgg cgtagccgcc ggcgaacacg cagcagagat agaccagact
     3181 gccggtgagc agcagggcac ttccggtcag cgcctgaccg gaagtacaac cctgcgccag
     3241 acggctggcg aacccggcga tgacgccgcc aacgatcgct agaaccgcgc gcgtcgcgcc
     3301 ggcgcaggcc gtgccgcgtt cgaggcgaac ctgctgtcga cccgacagcc aggccgacag
     3361 aaagccgccg agaaaggttc cggcgagcat gaagaccaga tagtagttca gcggcgactt
     3421 gccccaaccg ccgaaatact cggaagacag cacatgttcg ggagcgatac acatctggca
     3481 gaaagcggcg agacgggcca ttcccgccga cgcgcccagc cccgctccga gaatcagaaa
     3541 cgaggcgagc agggtgagtc cgagcaacag acccgcaaga tacggattca gatagggttg
     3601 aggagaggtg tttcctgcac tctttgattc tggggcatcc atggctcagc accccgcgct
     3661 ctttttcttc ttcggtgccg gttgggccgg agcggggctg gatggggccg ctggcgtggt
     3721 gatgggcgcg acgatcggtg gggagatgcg cggtgcgacg cctgttggtg ctgtagtccc
     3781 agttgatgac ggagtgagaa gcctctgctt ctcattgtcg gtccaccatg attccatgcc
     3841 gccgtgcaga gccttgatcg ggcgtttggt cttctgcgac agaatgccgg cgatcatcat
     3901 cgccagactg ccgtctcggt cggtgatgat cagcggaatg tcgccgacga gcagggaggg
     3961 atcggacaag gccgcggcga cgtcgaccgg ccgcgcctgt aacgggttgt aatccgcgat
     4021 ggcggcggcc ggtcgcagat cgatgatttc aaaggtgccg ggcagatcga gcttcaggcg
     4081 tctcagatcg gccggggcca ggcgatcggg gaggttgacg ctgcgcagtt gagtcggtgc
     4141 gggagtcgcg tggctgccgg ccgcggtcgc gccgaaaacc ggatacccgg cggcgatcca
     4201 ggcttcgctg ccgccttcga gacttgaggc ctttgaaaat ccgaggcgtt caagcacgcc
     4261 gaccgccatg ctcgaccggt aggccgagtt gcagacggcg accacgggct gctctttctc
     4321 gagtttgacc ggtgcggttt cgccgagatg attcagcggc aggttcaaca cctgtccgat
     4381 gcgcaggccc atccattccg tcggtagtcg gacgtcgacg atcagcgggg cgtcaccttt
     4441 ctgcatgccc tgatacaggg cttcgggagt ggtcatctgg ttccgtgcca cgggcagacc
     4501 ggcggtcttc caggcggtcc agtcgatggt tttggctgtg taacccacgc ggtggagccg
     4561 ccgaacggat tcagcggttt ctgccggtga gccgcacaga atcagattcg aaccccaggg
     4621 aaccatgatg ccgacccagg tttcgagacg cccgcgcacg ccgatgttca cggagttcgg
     4681 aatgtgcccg tccgcatacg tcttggcatc acgtacgtcg acgacataac cccggctcac
     4741 gtcggtaaga gcggcctgcg gagcgacgac ttcgggtgtc gccgcccagt cgaccagcgc
     4801 gggccctttg cggttcatct gggcgttgtg cttgaaatac tgtggagctt cggggagtcc
     4861 gtcgagaacc gcggcgatga aggcgctgcg actaccggcg tactgaagat agctgttgac
     4921 ctgcttctgc gcgcccagcg tcgaagtcgg ttcatcgctg aggtgagcgc cgcagagcga
     4981 acccgcgcca tgggccggaa agatcgatac cgagtcggga agtttcgaca acttgttggt
     5041 ccaggtgtcg aacagcatgc ccgccaacgc ggaggcactc gtcgtgccgc ccatcagatc
     5101 aggtcgaccg acacttccca caaacagggt gtcgccggtg aagagatgct tcggctcctt
     5161 cggggatgac ttcgcggcaa cgagagcgca gatgccgtcc ggcgtgtgac ccggcgtttc
     5221 gagaaacgag agcagggcgt cgccgacctc gagggtggtg ccgtcacgca gaggttcgaa
     5281 cggatactcg gcgccgctga cgtgcgacgc gtacatcttg cagcccagcg ccttcgccag
     5341 ttccgtgtgt ccggcgacga aatcggcatg ggaatgggtc agaaaaactc cgcgacaggt
     5401 caacccctct tttttcagca gttcgaggta agtgtcgata tcgcgtccag gatcgacgat
     5461 caatgcttct ttgccactga tcagcgcata ggagtagtga gacaatacgg caaggttata
     5521 ctggagaatg cgatatgcgc caccgtcgta ggtcgcgacc aggtcatact tggccgcggt
     5581 gtcgtcgtga gaggccgact cggtatcttt gatagtgccc gcgtgcaggg caaggctcca
     5641 gcagagggcc acgatcagaa ccaggcgccg ccaaagagaa aaagattgca taggatcctc
     5701 ctgtcgatgt tgaacggtag cgtacagaaa aagcatccgg aagggaaccc cccttccgga
     5761 tgctgtgccg tcttactctt cgtccgtctg cgcgccgagt ttcaactggc tgcgcgggag
     5821 ttttttggtg cagaagaacc agtcgtagca gtggacgcct tcggtcttct ggacccggtc
     5881 tttggcggca ccgcaggaac cgggaggagg aacgatgaca tcatcgcccg gttgccagtc
     5941 ggcggggcag gcgaccttgt gtgcatcggt ggtctgcaga gcaatgagca ggcgcttgag
     6001 ttcggcgaag ttgcggccgg cttcgagcgg gtaatacatc atcgcgcgga ttttcccggt
     6061 aggatcgatg tagaacaccg cacggaccgc tttggtatcg gcggcgcccg gatgaaccat
     6121 cccgtacaac ttggcgacgt tcatcttgat gtcttcgatc agcgggaaag tgacttcgac
     6181 attcttcatg ccgcgatagt gaatcttttc cttgatggtg cgaagccagg cgatgtgact
     6241 gtacatgctg tcgatggaaa ggcccaccag ctcgcagttc agcgccttga actgcttgta
     6301 cattttggcg aaggtcatga attcggtggt gcagaccggc gtaaaatcgg ccggatggct
     6361 gaagagaata acccattttc ccttgtaatc agccgggaaa ttgatctttc cctgggtggt
     6421 gtctgcggta aaagccggag cgggttcacc gatcaacgga atctgtcctt cggcaccaaa
     6481 accgaggccg gggacgagga gcgccagcag agccacaacg agaaaaacgg aacgtttcac
     6541 gatctacctc cgaaaatgta atttctgcct ctataagtat aggcaatctg tatcatatgc
     6601 ttaccacgga aggtctcgag gaatcaatgc tgaggatgct gtgctaaacc ggcattggaa
     6661 agcagattgg gaatggcagc tgggggagag gatgatgaaa ttggtcctgg acagtctcgg
     6721 cgaaggggaa ggatcggatt ggtgcctgcg cgccgatgtt cgatcatcgg agcgtctcac
     6781 gagtctggca ggccgtccgc ggagtgaggg aagcaaaggg atgctcggcc cgtccagacg
     6841 ggcactccct gctgacgcca aagtctcaaa gcctgtccca cgaaaaaagt gggttttcgc
     6901 caggctgaga gtcgcgtgac ttgcttcagc gggcggcttc gcataccatg accaccctat
     6961 gtttcgggac ggaccgggcg aagaacaaga tcagcgacag tatgcgaacc gggaactcat
     7021 tcgccggctg attccgttcg tgcggccgca tctggcgcag atgctgatcg ccgcgttgat
     7081 gatgggggtg acggtcgcgc tcgggttggc cggaccgctt ctgatacggc ggggcatcga
     7141 ccgcgatatt gccggtcgtg atctcgaagg gctcaagtgg acggcgtttc tcttcatcgt
     7201 cgtccagctc ggcactattc tcgtctacta ctttatggcc gtgcgtctcg agaaggtcgg
     7261 ccaggcagtc atggccaggc tgaagaagag tctatttgcc catctgctgg gcctttcggt
     7321 tgactacttc gatgtgaatc cggtgggcag gctgctgtcc cgcgtcgaga gcgacacgaa
     7381 caccctgcga cgactgttgt cccatacggc gctgcaggtg gcttccaatc tgttgatgct
     7441 gctcggcatg ctcggggtca tgttctgggc cgacgcgcgc ctcgccctgg ctacgatgct
     7501 gatgcttccc attctcgtcg ggctgcttta ctggtttcaa accaaggtgc gggcgaggtt
     7561 caaacgggtg cgcaagctgg cggccgaggt ttccgccttt gtagccgagc gggtcggggg
     7621 aatccgcctg attcaggcct tcggcatcga aaagcagatt cagagccgcc tggcgacggt
     7681 gaatgccgtg aagttcgagg cggcttacca cgcggaacag gccgtgatca tgtttttcaa
     7741 cggcattcag atggtacagg ttctgggtct tgccctggtg ctcgggttgg gtggttactg
     7801 ggtgacggcc ggctcgatga gcatcggaac ccttgtgttg tttctcaact acattcagcg
     7861 gttcttcggc ccgctgatgg cgctcagtga agaactcaac gtgacccagc gcgcgttcgc
     7921 ttcggccgac cgcatcttcg ggatcctcga tactccgtcg cgagtggcag agccaaccga
     7981 agccgtttcc gtgccgaagg cgtttcagac cctcgagttt cgtgaggtct ggtttgctta
     8041 taaag
//
LOCUS       DAORHD010000035         8043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8537_length_8043_cov_3.704056, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000035 DAORHD010000000
VERSION     DAORHD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8043
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8537_length_8043_cov_3.704056"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKO06_01455"
     CDS             <1..913
                     /locus_tag="PKO06_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68334.1"
                     /translation="MRKHAWALACGPILLGYHLECEETACAGLTLISRMISVAVRKIG
                     SQALFDLAPHLAIDRVMAFNTFDLCERVEKGLLPIMASIEQEATSLPAFADTAQRALE
                     SGAAHKTLSHRPSLAMRSLTNPNRVKKRLDPLYDPLRAVSGKPLKETASATREFGRMI
                     DEIQMRTTTPSSEWLLYLVTPTNLVLDFVIALATPNLKKAFEQDYVCRQTTRGVSVAY
                     VLAAYQHEFGKWPATLAEAETWMKVTFPLDLMTGDPMKYELVGTQSASLTSSGPDCTH
                     GTADDLSFLPFPSPLSNEPEQPAENEP"
     gene            918..1604
                     /locus_tag="PKO06_01460"
     CDS             918..1604
                     /locus_tag="PKO06_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68335.1"
                     /translation="MYLPHLTAIFQHSLNAGAAKKTLFQRPSFAMRALTDPERVKRRL
                     DSFFDHLRHVDGQPWSVVAPATASFTSLLHEVQTRSRTPMRFWAVYVFQPTHLVFDRL
                     ISELIPDCQKAMEQEYVSRSSTRGMGVAYAIAAFHHERQYWPATLAEAETWANASFPV
                     DVFTSAPYQYAVSASGTPSLVSPGLDLLYGTTDDLIYLLFPTVIASRSPDITHTEDTP
                     SSTSLGRRKR"
     gene            1609..2430
                     /locus_tag="PKO06_01465"
     CDS             1609..2430
                     /locus_tag="PKO06_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68336.1"
                     /translation="MIDRSQLVMLIPPEAVPPGSELADSLARWGNAAPLLIHGRQIVD
                     GIKRWSVFSPEQRATVPLIETSLASPFLARFLANASREWNIIETALLLQHLSESERQA
                     LAVSRRIALAPNLLNVFAYLSSLPSCWARVIDSALPVSVWRDLGALGNRLGEAVELML
                     TLSGTVAEKRMLAGLLRQAHLRHLLPSALASRDLSVLLTELQQLVSPRRDDVKKRYDE
                     AIADTDLPPNTELTVDPVFEKPGVTVKLHLRRQELARLERLRQSLDHLFARIPEL"
     gene            2434..3498
                     /locus_tag="PKO06_01470"
     CDS             2434..3498
                     /locus_tag="PKO06_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68337.1"
                     /translation="MLTTRTLFIDHAIAGHPRTAEIERGISYQKRELTSGERHPGGGI
                     LLTRNRGGFVKPCPGMKGAVCCGLWVVEWGMGCPFQCEYCVLQVYQTSGDLTLYVNWE
                     DCLEEVSRLRQQVRGPIRLCTGEFGDSLALEPDFPLNTLLARHTAGFAEFTLELKSKS
                     VCIESLLTLPETRHLIVSFSLNAPRIMTHLEHGTASLEQRLTAARALAERGIRLAFHF
                     DPIVALPGWETEYQETVAAMHRTLPAQADIAWISLGTFRFPRFFPDRALETFPGSILF
                     QEEFHPSFDGKLRYFRPLREQLYRHLSRLLNDAFPGVRTYLCMESEDVWERVTGKRRH
                     SGDLRCLLDQAVPCGQVTSA"
     gene            complement(3495..4418)
                     /locus_tag="PKO06_01475"
     CDS             complement(3495..4418)
                     /locus_tag="PKO06_01475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68338.1"
                     /translation="MRPEVHPIDPRWPFFSLDLELTTECDRNCAFCCRHIKREHGEMS
                     ESTWNRILAEARTHGSRIAFSGMGDPLRHPHWQEWLAQAREARLTAGVVVPGGSLSVS
                     VVEKLIQIHPSFVEVSCPTLRAPLYQQLQPRDDLPRVIEHIRSLRQTGGARFPLVVVG
                     LLTRLNQDEEPAFQRFWNDLGIQARMFPCHSRGHHLLRTDLILAAPVDAGGCGLMARH
                     SFIAWNGDALACCHDLDGSTSHGNVKQHSFLEIGSRRIERVAVATPFELCRGCDEIRR
                     LWPLPAGDSFPNSASARSRAMRRLARTIARF"
     gene            complement(4418..5494)
                     /locus_tag="PKO06_01480"
     CDS             complement(4418..5494)
                     /locus_tag="PKO06_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HNV68339.1"
                     /translation="MGAYYYPGWHHCPERDRAFPKKWSEWDLVYQATARFAGHEQPNL
                     PLSGRYDESDPASFPTRIDLARNAGVTHFIFAFYWSRGLQVLNQALTKGFLPCAAGAS
                     FRFAVMWANRMPRRVLPVRDPNAATLDPQRFVHTDPDDFLALITYLADHFFAQANYFR
                     WNGGLYFSIFDTSFFLRQMGMDGAQRAIASARQVLAARRLGPLHLAAIDPVPEFQPHL
                     REIGFDSMTHYMKLPDWKGPNTQDYRQLITQRIPEWETVSAESGLPYVPSVAPGWDAT
                     PRAVDYGKEKPQRYPWSPIVTGRSPEAFAEFVRAGVAFARRSHDPEPLLHIASWNEWS
                     EGHYLEPDQRYGHAWLEAVRRGSV"
     gene            5598..6614
                     /locus_tag="PKO06_01485"
     CDS             5598..6614
                     /locus_tag="PKO06_01485"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HNV68340.1"
                     /translation="MIHACAATEAKSSLWPFTYEPAPLGEQDVDVAITHCGICHSDLH
                     LLDNDWRITTFPFVPGHEIVGRITAIGAGVKHLELGQRVGIGWQCGACHECEFCGAGL
                     ENLCRRQRATCVGRHGGFADRVIVDSRFAFALPETLDSAEAAPLLCAGITVHAPLARH
                     LPEKGRRVGVVGIGGLGHLALQFARALGHQVTAISSNPKKEAEAKSLGAHHFAVIDEK
                     RSVNKFKGSFDLLLVSAIGTLDWQLLLELLAPRGKLCLVGVPDGALTIPPFLLIESQK
                     SICGSNIGSPNELRTMLAFAAEHKIRSRIELFPLNEVNEAIRRLRSNSLRYRAVLQMA
                     PVAG"
     gene            complement(6618..7421)
                     /locus_tag="PKO06_01490"
     CDS             complement(6618..7421)
                     /locus_tag="PKO06_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNV68341.1"
                     /translation="MPRKPNRVITLLTDFGLEDPFVGVMKGVMAGICRRLQFIDLTHA
                     VPPQNIARASFLLATSFAVFPSGTIHLAIVDPGVGGSRRAVAIRIHEGFLIGPDNGLF
                     SGVLARSPALEVRELSEPRFWRTRTPSATFHGRDVFAPAAAHLASGMALSRLGREVDA
                     ASLTLLPQAGWKTAENGAQGVVQDIDRFGNLITSIPASLIPVAGSWTVQVGAVTANGA
                     ATYASAAPGSLIALCGSHGYVEVASVNGNAATQTAAAVGTAVRLVYVSG"
     gene            complement(7437..7910)
                     /locus_tag="PKO06_01495"
     CDS             complement(7437..7910)
                     /locus_tag="PKO06_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68342.1"
                     /translation="MADKLRFEVFARFLVERFGACRIYDVAGGMGRLNQELTHLGCRV
                     TTFDSRHKHLEVTYDQREFALDVPCDCDLVVGLHPDAATRVIIEYAGQHRVPCAVVPC
                     CSDNSMSYKPWMRYLATLAEGVGLQVTEATLPLTGRNRVLLGYPIDKTQTSSSPS"
BASE COUNT         1660 a         2481 c         2336 g         1566 t
ORIGIN      
        1 aatgcgcaag catgcctggg cgctcgcctg cggaccgatt ctgctcgggt atcacctgga
       61 atgcgaggaa acggcctgcg ccggtctgac gctcatttcg cgcatgatca gcgtggcggt
      121 gcgcaaaatc ggctcgcaag ccctgttcga cctggccccc catctggcca tcgaccgcgt
      181 catggcgttc aacaccttcg acctctgtga gcgcgtcgaa aaggggctgt tgcccatcat
      241 ggcctcgatc gagcaggagg cgacctcact gcccgcattt gccgataccg cacagcgcgc
      301 tctggagagc ggagcggcac acaaaacgct gtcgcatcgt ccgtctctcg ccatgcgctc
      361 gctgacgaat cccaaccgag tcaaaaagcg cttggatcct ctgtatgacc cactccgtgc
      421 cgtatcgggc aaacccctga aagagaccgc ttcagccaca cgcgagttcg gacggatgat
      481 tgacgagatc cagatgcgga caaccacccc ttcgtccgag tggctactgt atctggtgac
      541 gccgacgaat ctggttctcg attttgtcat cgctctggcg acaccgaacc tgaaaaaagc
      601 atttgagcag gactacgtct gtcggcagac cacgcgtggg gtcagcgttg cttatgtcct
      661 ggccgcctac cagcacgagt tcggaaagtg gccggcgacc ctggctgaag cagagacctg
      721 gatgaaggtc acgtttcccc tcgatctcat gactggtgat ccgatgaagt acgagttggt
      781 gggaacacaa agtgccagcc tgaccagttc tggccccgat tgcactcacg gaaccgccga
      841 tgacctctct tttctgccgt ttcccagccc cctgtcgaac gagcccgagc agccagcgga
      901 aaacgagccg taagcaattg tatctgccac atctgaccgc cattttccag cacagcctga
      961 atgccggcgc agcgaagaag acgctgtttc agcgaccgtc gttcgccatg cgcgccctga
     1021 cggacccgga gcgcgtcaag cggcgtctcg attcgttttt tgatcaccta cgccatgtcg
     1081 atggccaacc ctggagcgtg gtcgccccag cgaccgcttc ctttacgagc cttcttcatg
     1141 aagtgcaaac gcgttccagg acgcccatgc gtttctgggc ggtgtatgtc tttcagccga
     1201 cacacctcgt tttcgatcgc ctgatctcag agttgatacc ggactgtcag aaggcgatgg
     1261 agcaggagta tgtgagccga tcatcgactc gcggaatggg cgtcgcctat gccatcgccg
     1321 cgtttcacca cgaacgacag tactggccgg cgacgctcgc cgaagcagaa acctgggcca
     1381 atgccagttt ccctgtcgat gtcttcacga gtgctcccta tcagtatgcc gtttccgcct
     1441 caggaacgcc cagcctggtc agcccggggc tcgacctgct gtatggcacg acggacgatc
     1501 tgatctacct gctttttcct accgtcattg cgtcccggtc gccagacatc acgcacacag
     1561 aagacacacc cagctcgacc agtctcgggc gaagaaagag ataacgttat gatcgaccgt
     1621 tctcaattgg tcatgctgat tccgccagaa gccgtgccgc caggttctga actggcagac
     1681 tcactcgctc gttgggggaa tgccgcgccc ctattgatac acggccgcca gatcgttgac
     1741 ggcatcaagc gctggagcgt gttttccccg gaacagcgag cgacggtacc gctgatcgag
     1801 acgtctctgg catcgccgtt tcttgccaga tttctggcca atgcctcacg cgagtggaac
     1861 atcatcgaga cggctctcct gcttcagcat ttgtccgagt cagagagaca agcgcttgcg
     1921 gtcagcagac gcattgcgct ggcaccgaat ctcctgaacg tgttcgcgta tctgtcgtcg
     1981 ctgccgtcct gctgggccag agtcatcgac agtgctcttc ccgtcagtgt ctggcgtgat
     2041 ctcggcgcgt tggggaatcg ccttggagaa gccgtggagt tgatgctgac attatcagga
     2101 accgttgcgg aaaaacgcat gcttgcaggt cttctgcggc aggctcacct ccgccatctt
     2161 ctgccttccg ctcttgccag ccgggatctt tccgttctcc tcaccgagct tcagcagttg
     2221 gtgagtccga gacgcgacga tgtgaagaag cgatatgacg aggctatcgc cgataccgat
     2281 cttccaccga acacggagtt gacggttgac ccggtcttcg agaaaccggg tgtgacggtg
     2341 aaactgcatc tgcgacgaca ggaactcgcc cgactggaga gactgcgtca gagcctcgac
     2401 cacctctttg cccgcattcc agaactgtaa gcgatgctga caacccggac actgttcatc
     2461 gatcacgcga tcgcggggca cccgcgaacg gccgagatcg agcgcggcat ttcgtatcaa
     2521 aaacgcgagt tgacctcagg agaaagacac ccgggcggtg gtattctgct gacccgcaat
     2581 cggggcggat tcgtcaaacc ctgccccggg atgaagggtg ccgtctgctg cgggctctgg
     2641 gtggtcgaat ggggcatggg gtgcccgttt cagtgcgagt attgtgttct gcaggtgtac
     2701 cagacgagcg gcgatctgac gctatacgta aactgggaag actgtctgga agaggtatct
     2761 cgacttcggc aacaggtacg cggaccgatt cgcctctgca cgggcgagtt tggcgattcg
     2821 ctcgcgctcg aacctgactt tccactcaac acgctgctgg caaggcacac ggccggtttt
     2881 gccgaattca cgctcgagct caagtcgaag agcgtctgca tcgaaagcct tctcaccctt
     2941 cccgagaccc gccatctgat tgtctccttt tccctcaatg cgccgcgcat catgacgcat
     3001 ctcgagcacg gcaccgcctc gctcgaacag cgattgaccg cggctcgagc gcttgccgaa
     3061 cgcggcattc gcctggcttt tcatttcgat ccgatcgtcg cactgccagg ctgggagact
     3121 gagtatcagg agacggtcgc tgccatgcac cgcaccctgc ccgctcaggc tgacattgcc
     3181 tggatctcac tgggaacatt tcggttcccc cgcttctttc ctgatcgagc cctcgaaacc
     3241 tttcccgggt cgatcctgtt tcaagaggag tttcatccgt cgttcgacgg caaactgcgc
     3301 tattttcggc ccttgcgcga acagttgtac cgccacctgt cgcgtttgct gaacgacgcg
     3361 tttccgggag tgcgtaccta tctctgcatg gaatcagaag atgtctggga acgagtcacc
     3421 gggaaacgtc gtcatagtgg cgacctgcgc tgcctgctcg atcaggcggt tccatgcggg
     3481 caggttacct ctgcctagaa acgggcgatg gttcttgcca atcggcgcat agcccgggag
     3541 cgagccgatg cactgtttgg gaaagaatcg ccggccggca gaggccagag acgcctgatt
     3601 tcgtcgcacc cgcggcagag ctcgaacgga gtcgccaccg ccacccgctc tattcgtctg
     3661 ctgcctattt cgagaaatga atgctgtttg acgtttccgt gacttgtact gccgtcgaga
     3721 tcgtggcagc aggccaaagc atcgccgttc caggcaatga aggaatgtcg tgccatcaga
     3781 ccgcagccgc cggcgtcaac aggtgcggcg agaatcagat cggtgcgtaa caggtgatgc
     3841 ccgcgactgt gacacgggaa cattctggcc tgaatgccga gatcgttcca gaaccgctga
     3901 aatgccggct cttcgtcctg attgagacgg gtcagcagac cgacgaccac cagagggaac
     3961 cgagcaccac ccgtctgtcg caaagaccgg atgtgttcga tcacgcgcgg gagatcatcg
     4021 cgaggctgta actgctgata cagcggagct cgcagggtcg ggcaggacac ttcaacaaac
     4081 gaagggtgta tctgaatcag tttttccacc acagacaccg acaatgagcc gcccggcacg
     4141 acgaccccgg cggtcaacct cgcttcgcga gcctgggcca gccactcctg ccagtgcggg
     4201 tgccgcaacg gatcgcccat acccgagaag gcaattcgag agccgtgtgt tcgggcttca
     4261 gcaagaattc gattccacgt cgactcggac atttctccgt gttcacgctt tatgtgacga
     4321 caacaaaacg cgcagttccg atcgcactcc gtcgtcagtt cgagatcgag actgaagaac
     4381 ggccaccgcg ggtcgatggg gtgaacttcg ggccgcatca cacgctgccc cggcgcacgg
     4441 cctcaagcca agcatggccg tagcgctgat ctggttcaag atagtggcct tcgctccatt
     4501 cgttccagga agcgatgtgc aacagaggct cagggtcgtg actacggcgc gcaaaagcga
     4561 ccccggcgcg aacaaactcg gcaaatgcct cgggcgaacg cccggtgacg atcggcgacc
     4621 acgggtaacg ctgcggtttc tccttgccgt agtcgaccgc gcgcggtgtc gcgtcccagc
     4681 caggtgcgac ggaaggcacg tagggaagac cgctttcagc actcaccgtt tcccattccg
     4741 ggatgcgttg cgtgatcagc tgacgatagt cctgggtgtt tgggcccttc cagtcaggca
     4801 gtttcatata gtgcgtcatg ctgtcgaagc cgatctcacg caaatgcggc tgaaactctg
     4861 gaaccggatc gatcgccgcc agatgcagcg ggccgagacg gcgggccgcc aacacctgtc
     4921 gtgcagacgc aatggctcgc tgtgcaccgt ccatacccat ctgacgcaga aagaagctgg
     4981 tatcgaaaat gctgaagtac aagccgccgt tccaacgaaa gtagtttgcc tgcgcaaaaa
     5041 agtgatccgc gagataggtg atcaacgcaa gaaaatcgtc gggatcggta tggacaaaac
     5101 gttgcgggtc cagggtggcg gcgtttggat cacgcacggg cagcactcga cgcggcatgc
     5161 gattggccca catcaccgca aaacgaaacg atgcgcctgc ggcacaggga agaaacccct
     5221 tggtgagagc ctgattcaga acctgcagac cgcgtgacca gtagaaggcg aagatgaaat
     5281 gggtcacacc agcgttccga gcaagatcga ttcgtgtggg aaaagacgcc ggatcacttt
     5341 catcgtatcg gccagagagc ggcagattcg gctgttcgtg gcctgcgaag cgcgcggtcg
     5401 cctggtacac cagatcccac tcgctccact ttttcggaaa ggcgcgatcg cgttcagggc
     5461 agtggtgcca ccccggatag tagtaagcgc cgatggcaag aggcgaaacg atggtgagac
     5521 tccggctggt gttttacgca ggtctctggt atgtacaaca gaaagagatc atttgaccag
     5581 tcatcggagg atttgccatg attcatgcct gtgccgctac tgaagccaag agttcactct
     5641 ggccgttcac ctatgagccg gctcccctgg gagaacagga cgtcgatgtc gcgatcacgc
     5701 actgcggcat ctgtcacagc gatctgcatc tactcgataa cgactggcgc atcacgacgt
     5761 ttccgttcgt tcccggccac gaaattgtcg gccgaatcac ggctatcgga gccggcgtca
     5821 agcatctcga actcggacag cgcgtcggca tcggctggca atgcggcgcc tgccatgagt
     5881 gcgagttctg tggggcgggg ctcgagaatc tctgccgccg acaacgtgcc acctgcgtcg
     5941 ggcgccatgg cggatttgcc gatcgcgtca tcgtcgacag tcgattcgca tttgcgcttc
     6001 ccgaaacgct tgattctgca gaagctgcgc cgctgctctg cgccggtatc accgtgcacg
     6061 ctccgctcgc gcggcatctg ccggaaaaag gtcggcgcgt cggggtggtc ggtatcggtg
     6121 gtctggggca tctggctctg cagttcgccc gggcgctcgg gcaccaggtc accgccattt
     6181 cgtcgaatcc caagaaagaa gcggaagcca aatcgctcgg ggcgcaccac ttcgccgtta
     6241 tcgacgagaa gcgttcggtc aacaagttca aaggcagctt cgatcttctg ctggtcagcg
     6301 ctatcggcac cctcgactgg cagttgctgc tcgaactgct ggccccgcgc ggcaagctct
     6361 gcctcgtcgg cgttcccgac ggggctctga ccattccgcc gtttctgttg atcgaaagtc
     6421 agaaatcaat ctgcggcagt aacatcggca gtccaaacga attgcgtacc atgctcgcct
     6481 ttgccgccga gcacaagatc agaagccgca tcgaactgtt tccgctgaac gaagtcaacg
     6541 aagcgattcg ccgcctgcgc agcaatagtc tgagatatcg ggccgtgctg cagatggccc
     6601 ctgtcgccgg ctgaccttta acccgacacg taaaccagtc tgaccgccgt tccgacagcg
     6661 gcggcggttt gggtcgccgc attgccgttc acggaagcga cttcaacgta gccatgactt
     6721 ccacacagcg cgatcagcga tccaggcgcg gcagaagcat aggtcgccgc accgttcgcc
     6781 gtgaccgcgc caacctgaac ggtccaggaa ccggcgaccg gaatcagcga tgccggaatg
     6841 ctagtgatca ggttgccaaa gcgatcgata tcctgcacca cgccttgcgc accgttctcg
     6901 gcggttttcc aaccggcctg cggcaacagc gtcagtgaag cggcgtccac ttctcgacca
     6961 agtcgcgaca gggccattcc tgaagccagg tgagccgccg cgggagcaaa cacatcacga
     7021 ccatgaaatg tcgcagacgg ggtgcgggtt cgccagaaac gcggctcgga cagttcgcgc
     7081 acttccaggg cggggctgcg cgccagcacc ccggaaaaca gaccgttatc gggaccgatc
     7141 agaaaaccct cgtgaatgcg tatagccacc gcgcggcgtg accctcccac acctgggtcg
     7201 acgatcgcga gatgaatcgt tcccgacggg aacacggcaa acgatgttgc cagcaggaac
     7261 gacgcccgcg cgatgttctg cggtggcacg gcatgcgtca ggtcgatgaa ctgcagacgg
     7321 cgacagattc ccgccatcac tcccttcatg acgccgacga acgggtcttc gagcccgaag
     7381 tcggtcagca gtgtgatcac gcgatttggt ttacgaggca tagtccaggt tctccatcac
     7441 gagggtgagc tagaggtctg cgttttatcg attggatatc caagcagcac acgatttcgc
     7501 ccggtcagcg gcagagtcgc ttcggtcacc tgcaggccaa ccccctcggc cagggtggcc
     7561 agatatcgca tccagggttt gtacgacatg ctgttatcag agcaacaggg aacgaccgcg
     7621 cacggaacgc ggtgctgacc ggcatactcg atgatcacgc gcgtggccgc atcgggatgc
     7681 agtcccacca ccaggtcaca atcgcacggc acatcgagtg cgaattcccg ttgatcgtag
     7741 gtgacctcga gatgtttgtg ccgggagtcg aacgtcgtca cccggcaccc gagatgcgtc
     7801 agctcctggt tcagtcgccc catgccgccc gccacatcgt agatacggca ggccccgaag
     7861 cgctcgacca gaaaccgggc aaagacctcg aaccgcagtt tgtcggccat atcacgggat
     7921 tctacatgta cccgcgcatc gttcgccaga acaaccacgg cagccagagc agcagcagag
     7981 ccgccagcat agccggaccc gccggtagat cataccgcaa cgccaccaac agtccgccgc
     8041 acg
//
LOCUS       DAORHD010000036         8037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8552_length_8037_cov_3.567026, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000036 DAORHD010000000
VERSION     DAORHD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8552_length_8037_cov_3.567026"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKO06_01500"
     CDS             <1..382
                     /locus_tag="PKO06_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68343.1"
                     /translation="YLELYLGDTRKRHVLNGVGTVVFIAGCMLLPGSHRMVQFLVDQR
                     YATYWVVKILTLAFIFGAGLCYTTIAEVAAVRRLLGKEDDANFQRTLFPQMFRVNAGA
                     LIIGLFLFAGLTLPVRFRSEGFLF"
     gene            484..1413
                     /locus_tag="PKO06_01505"
     CDS             484..1413
                     /locus_tag="PKO06_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68344.1"
                     /translation="MKRLLLGLCLLVAVLGVIDTPAFSQELTVYSMCPPRSLDWSNPR
                     ALMFTTAGNKLTFHHKDHKHSIGHVFIELKNGSERMLTGSVPTGDSNAVNKQLLLKEG
                     AGLGILFADMPGRLENTPDLDSELPDRYKSGRIAFVTFKLSQATYDRVKNFIAEYKAR
                     GYDKIYNGLNDPRRGLGAGCGTFGMACLEVAGLLHPTWKNIWYRHIDIAERLIGGKLG
                     GGKKVSVTDILAAGRWNRDGEPTRRLDLCDPNLVYLWINAQHDKLVFKQIHAGADGLV
                     LSQQVTPEKRQKAKGLVYDCTHVKTPTEPIFLK"
     gene            1723..2373
                     /locus_tag="PKO06_01510"
     CDS             1723..2373
                     /locus_tag="PKO06_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV68345.1"
                     /translation="MNQANPLPVMKPKPLLFVISGPSGSGKGTALDFIGREFAGSIER
                     SPTYTTRAKRNGEREGKDYHYVNEAQFSDLVAQKEIFEFTRTYNDELYGSPRCLINTD
                     NEKHLLVELDFNGFHRLRSISRRKVIGIFIMPPSIEELSKRILARQPETNLDRRLHRV
                     SEQIAHAWSYDYIVLNRDRDEFINRVRCVVASELYRLDGMGFLTENWHALDVTLNR"
     gene            2430..4382
                     /locus_tag="PKO06_01515"
     CDS             2430..4382
                     /locus_tag="PKO06_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68346.1"
                     /translation="MFRERRVVPAAAWFLLALLVSSAWALPPAKYHGGVRVVYVVPAD
                     REPHADYRERLDFVFRLIQDFFRRRMTEAGYLNADGQGKTFTLETESDGLLKVHLLDH
                     RPEGNLPDPALNHAFYQKNPYHIDAEMAKHLGLHISDSAVTVRITDMSFINTRQGLEL
                     APNCGVGGDAGGWMHEADHVFGNDLRYTDLPYPPLPFHAIGRDESEQTAIFNDVRYTN
                     IDAGGPYARPSPLRWRSDEWPEAQNAQVWEYATVFIGAMIHELGHGFGLHHCFVPTPA
                     SDGLTMIDSQLMGNGFRGIFASILPGQQFDARHHYPRHDPTRIPSSDFGTPVVLHPGQ
                     LANLNHHPLFNQERRPSDKTPPRTIRAFVRDGNSASACFAEDVERIPVTVDVADDQGP
                     EPRSGLSHVNILMDANQREYVEIPEASAHGASRHSLIFGLHPGEDRRQVFAPGLHSIS
                     FEVVDREGNKSRDCPWTLFISIRDHRLERWMMWEEWFAARGSTRREMLETSYLEQDDA
                     QVRPWDAESGKATGWRTFETGPFVHLSARTDEFFAHAPRDGARLAYAATMVESETERE
                     AVMRMGNDDYAQVYLNGERVLNEDVEKAPHDPFDSARYHSVPVRLRAGSNLLLVKVLD
                     VAGGSGFHVWFEETNGRPLKLKNDLP"
     gene            complement(4458..5189)
                     /locus_tag="PKO06_01520"
     CDS             complement(4458..5189)
                     /locus_tag="PKO06_01520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV68347.1"
                     /translation="MNFYTGISDHYDDIFQNTDEQVRFLTPHLAPLPKKIADLGCATG
                     KLANELAAQGHQVLGIDFDARMIDHARTEARSRGLATQFEVADIADLGKFVPTSHLDV
                     VSCFGNTLVHLPSPEAMEKLAAKIRATLVPGGWFVGQIINYDRILDQNITTLPTIDNE
                     QVRFDRKYIFTPGKPRFSFETTLTIKASEAEFKYSVMLFPLRQKQLADIFAQSGYQYC
                     EFFADAERHDWKPDSYALWFAARNQ"
     gene            5257..5382
                     /locus_tag="PKO06_01525"
     CDS             5257..5382
                     /locus_tag="PKO06_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68348.1"
                     /translation="MIPEFRQFASVQTHFEAEEMPIAEVKRKTRPVRLYELNRLR"
     gene            5819..6109
                     /locus_tag="PKO06_01530"
     CDS             5819..6109
                     /locus_tag="PKO06_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HNV68349.1"
                     /translation="MLHEPVAPVGKDLASDYKSRIGVWMFWLYAFIYASFVGINLLWP
                     TSMENIVFMGLNLAVFYGFGLIVFALVLALIYNHLSTSREAAMNAEEASKGA"
     gene            6113..>8037
                     /locus_tag="PKO06_01535"
     CDS             6113..>8037
                     /locus_tag="PKO06_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNV68350.1"
                     /translation="MELMPVILFLFFVGLVLGLSFYFGRQTKSATSYFAAGGTIHWAV
                     NGIAFAGDYLSAASFLGICGMIATAGYDGFLYSIGYLAGWIVALFVVAEPMKRLGKYT
                     FTDALDAKFNSKGIQLAAAISTLVVSLFYLIPQMVGAGVLVTPLLGLPHAAGVIMVGM
                     IVIIIVATAGMTSTTYVQFLKGGMLVVFSLALSIMVCQRGLTTTPDQAGTKPFYAYQT
                     LAATASGDTLTLADPSWQLVGKIDAGGATFVKLSKGGAVTYWKKETKEGEATVQLRET
                     QSLVVKPDGSKIINGAPASPENQLRQVGNLEEIRGKTGAEAVTGAIGPFELMSILGDS
                     STKARLWKEVKFKDGEDQVKIYTSYLVPGNKLMRPGMKFKVEGTTLEKIDFISLMLAL
                     FLGTAALPHILIRYYTVPSPACARKSTIVAIAAIGFFYVLTLYMGFGAMVNCVVNPAD
                     NNMSAPLLAKSFGVFLFSVISAIAFATVLGTVSGLIVAASGAVAHDIMDRYLALKMNE
                     KQKVMAGKVSAFVVGIIAIILGIIFQGVNVTFLVGLAFAVAASANLPSVIMLLFWKKT
                     TPHGIVASISTGIVVSIGIILFSPDMYALYGFDPKTAPIPFTNPGIFSIPASFFMLVA
                     VSLLTKAREGVALLPEL"
BASE COUNT         1609 a         2257 c         2407 g         1764 t
ORIGIN      
        1 gtatctcgag ctgtatctcg gtgatacgcg gaaacgacac gtgttaaacg gggttggaac
       61 ggtggttttt attgccggct gcatgttgtt gcccggcagc catcgcatgg tgcagtttct
      121 ggttgatcaa cgatacgcca cctactgggt ggtgaagatt ttgacgttgg cattcatctt
      181 cggtgccggc ctctgttaca cgaccattgc cgaagttgcc gccgtgcgac gactgcttgg
      241 aaaggaagac gatgccaact ttcagcgcac gttgtttccg cagatgtttc gagtgaacgc
      301 cggagctctc atcattggcc tgtttctctt tgccggtctg actttgccgg tgcggtttcg
      361 ctctgaaggg ttcctgttct agcgaacgac ggccgttgtc ttgacggttc gaggggcaat
      421 tcttacactt gtaccaacgc tgtcgatgaa cagcgggttt ttggtattgg aaaggagagt
      481 cgcatgaaac gtctgcttct tgggttatgc cttctggtcg cagtgttggg tgtcattgat
      541 actccagcct tcagccagga actgaccgtc tattcgatgt gtccgccgcg aagtctcgat
      601 tggtcgaacc cgcgtgcctt gatgtttacg acggcaggca acaagctgac ctttcatcac
      661 aaagaccaca agcattcgat tgggcacgtg ttcatcgaat tgaagaatgg cagcgaacgc
      721 atgctgaccg gaagcgtgcc gacgggtgac tcgaatgccg tcaacaaaca actcctgttg
      781 aaggaaggcg cggggctcgg cattctcttt gccgatatgc ctggacggct cgagaacaca
      841 cccgatctcg acagcgagct tcccgatcgg tacaagtcag gtcgcatcgc tttcgtcacg
      901 ttcaagctga gtcaggcgac ctatgatcgt gtgaagaact ttatcgctga gtacaaggcg
      961 cgtggctacg acaagatcta caacggtctc aacgacccac gccgcgggct gggtgccggc
     1021 tgtggtacct tcggtatggc ctgtctcgag gtcgcgggtc ttctgcaccc gacctggaag
     1081 aacatctggt accggcacat cgacatcgcc gagcggctga tcggcggcaa actgggcggc
     1141 ggcaagaaag tctcggtcac ggatatcctg gctgccggcc gctggaatcg cgatggcgaa
     1201 ccgacccgcc gactcgatct ctgcgacccg aatctggtct atctgtggat caacgcccag
     1261 catgacaaac tggtgttcaa gcagattcat gccggtgcgg acggtctggt actcagccaa
     1321 caggtgactc ccgaaaaacg ccagaaagcc aaagggctgg tgtacgactg cacgcatgtg
     1381 aaaaccccga ccgaaccgat ttttctcaaa tagttcccga attcgtccaa acgcccggtg
     1441 agagcaaggc tccgccgggc gttctctttg gcacgagttc gcaggcggtg aaccgcttgt
     1501 cacgcatggg cttcgaatac gagcaatgga caacgcgcat gggcgcagaa ggactcgtga
     1561 gacaagtcgc agacgaggct tttagaaggg tgtaccaccg cgatccgatg caaacccgta
     1621 tggccggcga ttgcacggtg gggtctttgc aggggatcgg taatctgata cagtgacggc
     1681 aacgccgtgc acgacattct cgaatagatc cgggggccag ccatgaatca ggcgaatccg
     1741 ttaccagtca tgaaaccgaa accgctgttg tttgtcatct cggggccgag cggctcggga
     1801 aagggtaccg cgctcgactt catcggccgc gagttcgcgg ggtcgatcga acgttcgccg
     1861 acctacacca ctcgcgccaa acggaatggc gagcgcgaag gcaaggacta tcactacgtc
     1921 aacgaggcgc agttttctga cctggtggcg cagaaagaga tcttcgagtt cacccgcacc
     1981 tataacgatg aattgtacgg gtcgcctcga tgtctcatca acaccgataa cgaaaaacac
     2041 ctgctggtcg aacttgattt caacggattt caccgtctgc gctcgatttc gcgccgcaag
     2101 gtcatcggca tcttcatcat gccgccctcg atcgaagagc tgtcgaagcg cattctggcc
     2161 cgtcaacccg agaccaacct cgatcgtcgt ctgcaccggg tgagcgagca gatcgctcat
     2221 gcctggtcct acgattacat cgtgctcaat cgcgaccgcg acgaattcat caaccgggtg
     2281 cgctgtgtcg tggcgtctga attgtaccgg ttggacggca tgggattcct caccgagaac
     2341 tggcatgcgc tcgacgtcac gctgaaccgg taggccgtca cgaaccccgc ttcgcagaac
     2401 tctccgcggg ggcacgcaag gagggaggca tgtttcgtga gcgtcgggtc gtgccggcgg
     2461 cggcgtggtt cctgctcgca ctgctggtct cgagcgcctg ggcacttccg ccggcgaagt
     2521 atcacggggg agtccgagtt gtgtatgtgg ttccggctga tcgcgagcct catgccgact
     2581 atcgtgaacg actcgatttc gtctttcgcc tgattcagga cttttttcgc cggcgcatga
     2641 ccgaggccgg ctatctcaat gccgatggcc agggcaagac gttcacgctt gagactgaat
     2701 ccgatggtct gctcaaagtg catctgctcg atcatcggcc cgaaggcaat ctccccgacc
     2761 ctgctctgaa tcacgcgttc taccagaaaa acccctatca catcgatgcg gagatggcga
     2821 agcatctggg tctgcacatc agcgacagcg ccgtgacggt gcggattacc gacatgtcgt
     2881 tcatcaatac ccgacagggg ctcgaactcg ctcccaactg cggagtcggc ggggatgcgg
     2941 gcggctggat gcacgaagcg gaccacgtgt tcgggaatga tctccgatac acagatctgc
     3001 cgtacccacc gttgcctttt cacgcgatcg gccgcgatga atcagagcag acggcgattt
     3061 tcaacgatgt gcggtatacg aatatcgacg ccggcgggcc ctatgcccgc ccgtccccgt
     3121 tgcgctggcg gtccgatgag tggcccgagg cgcagaacgc tcaggtgtgg gaatacgcca
     3181 ccgtgttcat cggggccatg attcacgagc ttgggcatgg gttcgggctt catcactgct
     3241 ttgttcccac ccctgcctcg gatggattga ccatgatcga ctcccagttg atgggaaacg
     3301 gcttccgcgg cattttcgct tcgatactgc caggacagca gtttgacgcg cgtcatcatt
     3361 acccccgaca cgatccgacg cggattccct cttcggattt cggcacgccg gttgttctgc
     3421 atcccggaca actcgccaac ctgaatcacc acccgctctt caatcaggag agaaggccct
     3481 ctgataagac accacctcgg acgattcgcg cattcgtcag agacggcaat tccgcttccg
     3541 cctgtttcgc tgaggatgtc gagcggattc cggtgaccgt cgacgtggca gatgaccagg
     3601 ggccggagcc gcgcagcggc ttgagccatg tgaacatcct gatggatgcc aaccagcgtg
     3661 agtatgtgga gattccggaa gcgtcggcgc atggcgcatc tcgtcactcg ttgattttcg
     3721 gtcttcaccc cggcgaggat cgtcgacagg tgtttgcgcc tggtctgcac tcgatcagtt
     3781 ttgaggtcgt cgatcgtgag gggaacaagt cgagagactg tccctggacg ctctttatca
     3841 gcattcgcga tcaccgtctg gaacgctgga tgatgtggga agaatggttt gccgcccgag
     3901 gctcgaccag gcgagagatg ctcgaaacgt cctatctcga acaggatgac gcgcaggtga
     3961 gaccatggga tgcggagagc ggaaaggcca ccgggtggcg gaccttcgag accgggccgt
     4021 tcgtgcatct gagcgcgcga accgacgagt tctttgcgca cgcgcctcgg gatggtgccc
     4081 gtctggcgta cgcggcgacg atggtcgagt cagagaccga acgagaagcg gtgatgcgga
     4141 tggggaacga tgactatgcg caagtgtatc tcaatggcga acgggttctg aacgaagatg
     4201 tcgagaaagc gccgcatgac ccgttcgatt ccgctcgtta tcattcggtg ccggtgaggt
     4261 tgagagccgg tagcaatctg cttctggtca aggttctcga tgtggcgggc ggcagtggtt
     4321 ttcacgtctg gtttgaagag acgaacggca ggccgctcaa actgaaaaac gacctgccgt
     4381 aaagccgcga tttaaagaga aaatgcccgg ttagaagccg gtactattgg cgcgcacgtg
     4441 ttgatcggga cgatcgtcta ctgattgcgg gcggcgaacc agagggcata cgagtcgggt
     4501 ttccagtcgt gccgttcggc gtcggcgaaa aactcgcagt actggtagcc actctgggca
     4561 aagatgtcag ccagctgttt ctggcgaagt ggaaacaaca tgaccgagta tttgaactcg
     4621 gcctcgctgg ccttgatggt cagcgtcgtt tcaaaagaaa aacggggctt gcccggggtg
     4681 aagatatact tgcggtcgaa acgcacctgc tcgttatcga ttgtgggcag tgtggtgatg
     4741 ttctggtcga gaatgcggtc gtaattgatg atctggccga cgaaccagcc gcccggaacg
     4801 agcgtggcac gaatctttgc tgccagcttt tccatggctt cggggcttgg gagatgaacg
     4861 agggtgttgc cgaagcagga aaccacgtcg agatgcgatg tgggcacgaa cttgccgaga
     4921 tcggcaatgt cggcgacttc gaactgtgtg gcgagtcctc gactgcgtgc ttccgtgcgc
     4981 gcgtgatcga tcatgcgtgc atcgaaatcg atgccgagga cctggtggcc ctgggctgcc
     5041 agctcgttgg ccagcttgcc ggtggcgcag ccgagatcgg cgatcttttt cggcaggggc
     5101 gccagatgcg gcgtcagaaa ccgcacctgc tcgtccgtgt tctggaaaat atcgtcgtaa
     5161 tgatcggaga taccggtgta gaagttcata cgggaacgat acctcatccc cccggccgca
     5221 ggcaagttcc tgctcgaagc ggcgatctgt cgcggtatga tcccggagtt ccgtcagttc
     5281 gcctcggttc agacgcattt cgaggcagaa gagatgccga tcgcggaagt gaagcgcaag
     5341 acacggccgg tgcgtctcta cgagttgaat cgcctgcgct gaagagtgag gggcggattc
     5401 ctgaatcgct gccgcagaaa cgggcgtttc tgcgctcgtc cgcaggcgaa cgggacgtcg
     5461 agtttgaaaa gtcctcgtcg atgattgctc gatgtcgctt gctgacttct ctgccggaaa
     5521 aaacaagttg tgaaaccgtc agagctctgt tattatccag accttctcgc tagtagagtt
     5581 tcgaaaaata gaacggcaac gaggagtttt tcgcgtcgcg ggtggtacag cccatacgca
     5641 gtctatgcaa gagagccccc gtctgttccg gccatcatga gccgtcacgg tctgcccgat
     5701 ctcgaatgcg gaccactcat gaccaagcaa aatcaacgga agcgaggatc agtcgggtcg
     5761 tatttttccg cctggtccct ctctccgtaa tgcatgcgaa atccccagga ggacaccgat
     5821 gttacatgaa cccgtcgcac ccgtaggaaa agacctcgcg tcagattaca agagccgtat
     5881 tggcgtctgg atgttctggc tgtatgcgtt tatctacgcc agctttgtcg gcatcaatct
     5941 gttgtggccc accagcatgg aaaacatcgt cttcatgggg ctgaacctcg ccgttttcta
     6001 cgggttcggg cttatcgtgt ttgcgctggt tctggcgctg atctacaacc acctgtcgac
     6061 gtcgcgcgaa gcggcgatga acgctgaaga agcgtcgaag ggagcctgag ccatggaact
     6121 catgccggtc attctctttc tcttcttcgt cgggttggtt ctcgggcttt ctttctattt
     6181 cggccgtcag accaagtcgg cgaccagcta cttcgcggcc ggaggcacga ttcactgggc
     6241 cgtcaacggc atcgcgtttg ccggcgatta tctgtcagcc gcgtcctttc tcggcatctg
     6301 cggcatgatc gcgacggcgg gctatgatgg gtttctctac tcgatcggct acctggccgg
     6361 ctggatcgtg gccctcttcg tcgtcgccga gccgatgaag cgtctcggta aatacacgtt
     6421 caccgatgcg ctcgatgcca agttcaactc gaagggcatt cagctggcgg cagcgatcag
     6481 tacgctggtc gtctcgttgt tctacctgat tccccagatg gtcggcgccg gggttctggt
     6541 gacgcctttg ctgggcctgc ctcacgctgc cggggtcatc atggtcggta tgatcgtcat
     6601 catcatcgtg gcaaccgcgg gtatgacctc gacgacctac gtgcagtttc tgaaaggcgg
     6661 catgctggtt gtcttctcgc tggctttgag catcatggtc tgccagcgcg gcctcaccac
     6721 aacccctgac caggctggca ccaagccgtt ttacgcgtat cagaccctgg cggctacggc
     6781 gagcggcgac acgctgacgc tcgctgaccc atcgtggcag ttggtgggca agatcgatgc
     6841 gggcggagcg accttcgtga agctgtcgaa gggcggagcc gtcacctact ggaagaagga
     6901 aacgaaagaa ggcgaggcga ccgtacaact cagggaaacg caatcgctgg tcgtgaagcc
     6961 agatggaagt aaaatcatca acggtgctcc cgcgtcgccg gaaaaccagc tgcgccaggt
     7021 cggcaacctc gaggagattc gtggtaaaac cggcgccgaa gcggttactg gagcaatcgg
     7081 tcccttcgaa ctgatgtcga ttctcggcga ttcctcgaca aaagcccggc tgtggaaaga
     7141 agtgaagttc aaggatggcg aggaccaggt gaagatctac acgtcctacc tggtgcccgg
     7201 gaacaagctg atgcgaccgg gcatgaagtt caaggtcgaa ggcacgacgc tcgagaagat
     7261 cgacttcatt tcgttgatgc tggctctttt ccttggtaca gccgcgcttc cgcacattct
     7321 gatccggtac tataccgttc cgagtcctgc gtgtgcgcgc aaatcaacca tcgtcgcgat
     7381 cgcggcaatc ggctttttct acgttctgac cttgtacatg ggcttcggcg ccatggtgaa
     7441 ctgcgtcgtg aacccggccg acaacaatat gtccgcgcct ctgctcgcca agtcgttcgg
     7501 cgtcttcctg ttttcggtga tctcggccat tgccttcgcc accgtgctcg gaacggtcag
     7561 cggtctcatc gttgccgcat ccggggctgt cgctcacgac atcatggatc gatacctggc
     7621 gctgaagatg aacgaaaagc agaaggtcat ggccggcaag gtctcggcct tcgtggtcgg
     7681 catcatcgcg atcatcctgg gcattatctt ccagggtgtg aacgtcacgt tcctggtcgg
     7741 tctggcgttt gcagtggcgg cctctgccaa cctgccctcg gtcatcatgc tgctgttctg
     7801 gaagaagacc acgccgcacg gaatcgtcgc gtcgatcagc accggcatcg tggtctcgat
     7861 cggcatcatc ctgttctcgc ccgacatgta tgcgctctat ggtttcgacc cgaagacggc
     7921 gccgattccg ttcaccaatc ctggcatctt ctcgattccc gccagtttct tcatgctggt
     7981 cgcggtgtcg ctgctgacga aggcccgcga aggtgtcgcg ctgctgcccg aactcga
//
LOCUS       DAORHD010000037         8032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8563_length_8032_cov_3.541056, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000037 DAORHD010000000
VERSION     DAORHD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8563_length_8032_cov_3.541056"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKO06_01540"
     CDS             complement(<1..484)
                     /locus_tag="PKO06_01540"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311967.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HNV68351.1"
                     /translation="MNGNLHGRHLLTLEDYSADEINYLLDLSAELKRLKRVGIKPRNL
                     DGKNVALIFEKASTRTRCAFVCACYDEGAHAEYLGKDDIQLGKKETVADTARVLGRMF
                     DGIQFRGFKHETVEELAKYAGVPVWNGLTDKFHPTQVLADFQTVQEQFGRVKGITFAY
                     V"
     gene            complement(597..932)
                     /locus_tag="PKO06_01545"
     CDS             complement(597..932)
                     /locus_tag="PKO06_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68352.1"
                     /translation="MPLSNYPTALLTLILTTIIEGVTCLFRFGFKLESTRDTASWVAP
                     LTFGVRIHHGYIGVLLCVVTWLWVRREPTRRALLIIGSALALSDLIHHFVVLWLVTGD
                     PQFDLTYPR"
     gene            1011..2078
                     /locus_tag="PKO06_01550"
     CDS             1011..2078
                     /locus_tag="PKO06_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNV68353.1"
                     /translation="MGRKSGGENRMVSVLRELQKVQGPSGFTDEAIAHVAEIARKAGI
                     RAERTLKGGLLVGQHPQPRFLVAGHLDTLGAMVCQINGDGTLAITKIGGPLLPSFEGE
                     YVTVHTMSGKLFRGTLLLNNPAAHVNRDAEKAERKPENMHIRLDAVVSSKKDVRDLGI
                     EIGDFICFDTRFEYVDTGFIKSRFLDDRAGCAVMIDTFLTLGRETLEQLPVAFFFSNY
                     EEVGHGACAGFPESIEEMLVVDMGVVGDRVDGDELSVSICPKDSSGPYDYQMRCELVS
                     LAKEGKIPHKIDVFPYYGSDGSAALRAGRNIRVGLIGPGVSASHGMERTHEKGLVATR
                     DLVLAWLEARQESPQPRRKKR"
     gene            2102..2833
                     /locus_tag="PKO06_01555"
     CDS             2102..2833
                     /locus_tag="PKO06_01555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV68354.1"
                     /translation="MLISIIVPAFNEERLLPACLKAIDAARAKFTDLGWDSEVIVCNN
                     NSTDRTGEVAEKLGARVVFEPVNRISTARNAGARAARGDWLVFVDADSEPPPGLFQAV
                     ARHIRCGKAIGGGALMRFPDKTPIGELSLILWNWCSRLWRLGAGAFLFVEAAAFQAIG
                     GFDEALYASEEIRFSIKLGLLGRRSKRQFVIIHKPRQLTSSRKLRLYSWRELVPQIAR
                     MVVSLPLAVRTREACAFWYDGRREP"
     gene            2894..4336
                     /locus_tag="PKO06_01560"
     CDS             2894..4336
                     /locus_tag="PKO06_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV68355.1"
                     /translation="MNPLLSATGPRSPLVERRRYPRLRALIDHPWFGVFIFFLILLSI
                     GLLVYEVFFSPSPSVLEWVHLTNDGLLLLFLVELILRWLCSATTRRFVASHWVEILAV
                     IPMFRIFRLSRVFLLVRLLRVFSLGALMQRRLAVYSNALGHRWVEYLLIFGILLFALI
                     FGSVGLAAYEVGPDSPLQSHSDAFWKALFSLITGEYADFPRSLGGKLVFCLLSLFAMG
                     IFAMITGTISAVMIEKLKENAMLKSENPEDLSGHIVICGYSSKVAILIEEFLASEDFR
                     SADFLLVSEHVDLEELRASNVPLNRIQVLREDFTRIETLRKANLNQARTAIILSEAGK
                     GRSHSDIDARTILAALTIERLNKGIHTCAEIYQPEFIDHLKMGGVDDVVVQGDVSGRL
                     LARLASTRGLQPFFKDLLTAKSGNHLKYVPVPQEYVGKPMNEVLPSLFRATGAILVGV
                     RDLKQEVLINPQKRILATGDELLVICPPGA"
     gene            complement(4448..6511)
                     /locus_tag="PKO06_01565"
     CDS             complement(4448..6511)
                     /locus_tag="PKO06_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68356.1"
                     /translation="MNSIIQRAGMFLLALLLVLPITADAWGPMTHLTVNQRAYEQAVR
                     EIGNQLAIPRELADDFIGGGPCPDIKSQGGASFPRDFHGDPDTVVRMIELAKQDPKFG
                     QADVVMALGWAGHVFGEVWTAHAEDGYANNKVTIPVEKSAGINHQINELCLDVLMYHR
                     NRESLKRKHLSIPIRLLEAAMIKEQENKPELPRMSAAEIKTISSKFLPTVVGIRVIAE
                     YLMRERPDLLDEMHQFFSDSEEQIDGSVQQVVRLLKEHGLRTSKTKTKSLDSSEKVKL
                     VLEGSFSNKFKTWAFSALGRVLQSDGANDIFTVLGYRLVKGALFTPAFHDPFLDKAKT
                     MGAASVWGNPNHSRIVTRFTEALLSRGDLTFPEIIAYAMEGFEPGTAEKQKQQSQFKQ
                     AGLNASGRKPVSSAQVLEALQEVERVEALQREWPWFWPWRPSDLRVAQTREQAGRLLT
                     NYFEDTGVSGSLGSRLQSFKQQNQQLRGAIWSYRMTAWYQPFKKWDQMKSLSALSDTY
                     AADKSFFAEVLRVQTQLAAQGNDPARLAGMKAETEQRLRETENALRNAKAQLEQLPIW
                     SLIKRDRKKDEIKKIEQQLADLKSRLEVLQALGETSPSTASSTTPASAPQASVVDLEL
                     GRNYTLAEAQKEYESAYAAYAALAAHQLQDTPAVKAALEKLDQARRVRESVIARLEAE
                     KAAGY"
     gene            6674..>8032
                     /locus_tag="PKO06_01570"
     CDS             6674..>8032
                     /locus_tag="PKO06_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNV68357.1"
                     /translation="MKKASAKSATPKPKKEGPRSSAHGVCWNLGDLYKDLKDKQIESH
                     YQTALKRAREFEKKYKGKLAPKPKLSGKQLGQMVQEYEEILMLRDRPHIYAHLLFASD
                     TQNPDYGRKLREMQNRSTEITKHLIFVDLEWCAVADADAKKLLADAAVKKYRHYLEAS
                     RRYKPHLLSEAEEKVIDEIENTGSRAFTRLFDELLGAMNFTVKVKGETRTMSEQEALS
                     LLYNPDRDTRQAAAQALTEGFKAGLKPITFIFNTLVDHHTVVDRLRSYADPMASRNLS
                     NDIEPKTVDALLKTCDANTGLVSRYYKLKAKLLGLKKLYDYDRYAPISADMPSCSWAE
                     SKELIIDAYREFSPELGRIVEEFYRKNWIDAELRPGKRGGAFCAGTTPDVHPYILTNY
                     SDKLRDAVTMAHELGHGVHQYLSRKQGYLQSDTPLTLAETASVFGEMLTFHRLMGRQT
                     NPA"
BASE COUNT         1658 a         2248 c         2333 g         1793 t
ORIGIN      
        1 cgacgtaagc gaaggtaatg cccttcacgc ggccgaactg ttcctgcacc gtctggaagt
       61 cggcgagcac ctgggtcggg tggaacttgt cggtcaggcc gttccagacc ggaacaccgg
      121 catacttggc cagttcttcg acggtttcat gtttgaaccc gcggaactgg atgccatcga
      181 acatgcggcc gagcacgcgg gcggtgtcgg cgacggtttc cttcttcccg agctgaatat
      241 cgtctttacc gagatattcg gcgtgagcgc cttcgtcgta gcaggcacag acaaacgcgc
      301 agcgggtgcg ggtcgaggcc ttctcgaaaa tcagggcgac gttcttgcca tcgaggttgc
      361 ggggcttgat gccaactctc ttcagccgct tcaactcagc cgaaagatcg agcagataat
      421 tgatttcatc agcggaataa tcttcgagag tgaggaggtg acgaccgtgc aggttgccgt
      481 tcatggtgga ttcttccttt ccagcaagta atttttcttc cgtgaggcgg aacatcacgc
      541 gttcggaatc tctctttgaa tagcattccc cgggaggttt ggcaactccc acccgatcag
      601 cgcgggtagg tcagatcgaa ctgcggatcg cctgtcacca gccagagaac gacgaagtgg
      661 tggatcaaat cagagagcgc gagcgcgctc ccgatgatca gcagcgccct tcgggtcggt
      721 tctcgacgca cccagagcca ggtcacgacg cagagcaaaa ctccgatgta cccgtggtga
      781 atgcgaaccc cgaaggtcag tggcgcaacc cacgacgcgg tatctcgtgt cgattcaagc
      841 ttgaagccaa accgaaacag gcacgtgacc ccttcgatga tggtggtcaa aatcagcgtc
      901 aggagggccg ttggatagtt ggacaggggc attgtagttg atctttcgtg atgaatcgag
      961 tctcgtgttc ctatggtatc atggggaaaa tcgcaggtga ggagtggcac atggggcgca
     1021 agagtggtgg cgagaatcgt atggtgagcg tgttgcggga gttgcagaaa gtgcagggac
     1081 cgtcggggtt taccgacgag gccattgcgc atgtggccga gattgcccgc aaggcaggaa
     1141 ttcgggccga acgcacgctg aagggcgggt tgctggtcgg gcagcatccg cagccgcggt
     1201 ttctggtcgc gggccacctg gatacgcttg gcgcgatggt gtgccagatc aacggcgacg
     1261 gcactctcgc gattaccaag attggcgggc cgctgttgcc ttcgttcgaa ggcgaatacg
     1321 ttaccgttca caccatgtcg ggaaaactgt ttcgtggcac tctgctgctg aacaacccgg
     1381 cagcgcatgt gaatcgtgat gccgaaaagg ctgaacgcaa gcctgagaac atgcacatca
     1441 gactggacgc tgtcgtcagc tcaaaaaagg atgtacgaga tcttggtatc gaaatcggtg
     1501 atttcatctg ctttgacacc cggtttgaat atgtcgatac agggttcatc aaatcgcgtt
     1561 ttctcgatga tcgggccggc tgcgccgtca tgatcgacac gtttttgacc ctgggtcgtg
     1621 aaaccctcga acaactgccc gtcgccttct tcttctcgaa ttacgaagaa gtcggtcacg
     1681 gggcctgcgc ggggttcccc gagtcgatcg aagagatgct ggtcgtcgac atgggcgtgg
     1741 tcggtgatcg ggtcgatggt gatgaattat ctgtttccat ctgcccgaaa gactcttcgg
     1801 gcccgtatga ttatcagatg cgctgcgaac tcgtcagcct ggccaaggaa ggcaagattc
     1861 cgcacaagat cgacgtgttc ccctactacg gctcggacgg atcagccgca cttcgcgctg
     1921 gtcgcaatat tcgcgtcggt ctgattggtc ccggcgtctc ggcctcacat ggtatggagc
     1981 gcacgcatga aaaggggctg gtcgctaccc gcgacctggt actggcgtgg ctcgaagccc
     2041 gacaggaatc accccaaccc cggcgaaaaa aacgctgact ctctgaggtc agccagacaa
     2101 catgctcatc tccatcatcg ttccggcttt taacgaagaa cggttgttgc ccgcctgcct
     2161 caaggcgatc gatgccgcga gagcgaagtt taccgatctc ggctgggatt cagaggtgat
     2221 cgtctgcaat aacaactcca cggatcgtac cggtgaagtt gccgaaaaac ttggggctcg
     2281 tgtggtcttc gagccggtga atcgcatcag tactgcgcgc aacgccggtg ccagggccgc
     2341 tcggggtgac tggcttgtct tcgtggatgc tgattcagag ccgcccccgg ggctttttca
     2401 agccgtggcg cgacatatca gatgcggcaa agccatcggc ggcggtgctc tgatgcgctt
     2461 tccggacaaa accccgatcg gagaactttc gctgatcctc tggaactggt gcagccgctt
     2521 atggcgtctc ggtgctggtg cgttcctgtt cgtcgaggcc gcggctttcc aagccatcgg
     2581 tggtttcgac gaagcattgt atgcatctga agagattcgc ttcagtatca agctcggact
     2641 tctgggccga aggtcgaagc ggcagtttgt tatcattcac aaacctcgac agctcacttc
     2701 ttcgcgaaaa ttgagattgt actcctggcg cgagctggtg ccgcagatcg cacgcatggt
     2761 cgtgtcgctg cccttggcgg ttcgtacccg cgaagcctgc gctttctggt atgacggtcg
     2821 tcgtgaaccc tgatccgacc attcctgaca gcttttgttt gcaggttctg aacggtttcg
     2881 ctacaatcgg ggcatgaatc cgcttctttc ggcaaccgga cctcgttctc cgttggtcga
     2941 gcgccgacga tatccgcgcc tgcgggcatt gatcgatcat ccctggtttg gggtgttcat
     3001 cttttttctg attttgctgt ccatcggtct gctcgtctat gaagtcttct tttctccgtc
     3061 gccgtccgtg ctcgagtggg ttcacctgac gaatgacggc ctgttgctgc tgtttctggt
     3121 agagctgatt ctgcgctggc tctgctcggc aacgaccagg cgcttcgtgg catcgcactg
     3181 ggtcgagatt ctggctgtca tcccgatgtt ccgtatcttc cgactcagcc gggtttttct
     3241 gctggtccga ctgctgcgcg tattctctct cggagcactg atgcagcgcc ggttggccgt
     3301 gtacagcaat gctctggggc atcgatgggt tgagtatctg ctgattttcg ggattctgct
     3361 gttcgccctg atcttcggtt ccgtcgggct cgccgcttat gaagtcggtc ctgactcacc
     3421 actgcagagc cattcagatg ccttctggaa ggcgctgttt tcgttaatca ccggagagta
     3481 tgcggacttt ccgcgatcgc tgggcggcaa gctggtcttt tgtcttctgt cgctgtttgc
     3541 aatgggcatc tttgccatga tcaccgggac gatttctgcc gtcatgatcg agaaactcaa
     3601 ggagaacgcc atgctgaaaa gtgaaaatcc tgaagatctt tccggacaca ttgtcatttg
     3661 cggatacagc agcaaggtgg cgattcttat cgaagagttc ctggcaagcg aagactttcg
     3721 cagtgcggat ttcctgctcg tttcggagca tgtggacctc gaagagctgc gtgcctccaa
     3781 tgtgccgctg aatcgcattc aggtgctgcg tgaagatttc acccgaatcg aaactctccg
     3841 caaagcgaat ctcaatcagg cgcgaaccgc cattatcctc tctgaggctg gaaaggggcg
     3901 atcacacagc gacattgatg cccgaaccat tctggcggcg ctcacgatcg agcgtctcaa
     3961 caagggtatt cacacctgcg cggaaatcta tcagcctgaa ttcatcgatc atctcaagat
     4021 gggcggcgtc gatgacgtcg tggtccaggg cgacgtttcg ggacgtctgc tcgcccggtt
     4081 ggcatcaact cgcgggctgc agcccttctt caaggacctc ctgaccgcca agagcgggaa
     4141 ccatctgaaa tacgtcccgg tgccgcagga gtacgtcggc aagccgatga acgaggtgtt
     4201 gccgtctctg tttcgagcca ccggagcgat tctggtcgga gtgcgcgatt tgaaacagga
     4261 ggtcctgatc aaccctcaga agcgcatttt ggctaccggc gacgaactcc tggtcatctg
     4321 tccgcctggt gcctgagtcc accggggaac gactgcccat catctctccg actgttgcct
     4381 gaaagagcga cgcccgagac acacatgtct cgggcgtcgc tctcttctca ggaaaactca
     4441 gggcagatca gtacccagcg gctttctcgg cttccagtcg agcgatgacg gattcgcgca
     4501 cgcgtcgggc ttggtccagc ttttcgagag ctgctttcac ggccggggta tcttgcagtt
     4561 gatgagcagc caaagcggcg taggcggcat acgcggactc atactctttc tgcgcctcgg
     4621 ccagcgtgta gttccgtccc agttcgagat cgacgacaga ggcctggggt gcagaagctg
     4681 gcgttgttga ggaagcggtc gagggactgg tctcacccaa tgcctgcaga acctcgagac
     4741 gcgatttcaa gtcagcgagc tgttgctcga tctttttgat ctcgtctttc ttgcggtcgc
     4801 gcttgatcag gctccagatt ggcaactgct cgagttgagc cttggcattg cgcagcgcat
     4861 tctcggtctc acgcaaccgc tgttccgtct cggctttcat accagcgagt ctggccgggt
     4921 cgttcccctg ggccgcgagc tgcgtctgca cacgaagcac ttcggcgaag aatgacttgt
     4981 ctgccgcata ggtgtcggat agcgccgaca ggcttttcat ctgatcccac ttcttgaatg
     5041 gctggtacca ggccgtcatg cgatacgacc agatcgcgcc gcgcaactgc tgattctgct
     5101 gtttgaatga ctgcaggcgc gaacccagac tgccggaaac gccggtatct tcgaagtagt
     5161 tcgtgagcag gcgaccagct tgttcacggg tttgcgccac ccgcagatcg ctggggcgcc
     5221 agggccagaa ccacggccac tcacgctgca gggcttcgac gcgttcgact tcttgcagag
     5281 cttcgagcac ctgagccgat gacacgggct tgcgaccgga agcgttcagg ccagcctgtt
     5341 tgaattgcga ttgctgtttc tgcttttccg ctgtgccggg ttcaaatcct tccatcgcat
     5401 aggcgatgat ttccggaaag gtcagatcgc cacgactgag caaagcctcg gtaaaacggg
     5461 tgacgatgcg actgtggttc gggttccccc agacactggc cgcgcccatg gtcttcgcct
     5521 tgtccaggaa aggatcatgg aaagccggag taaacagggc gcccttcacc aggcggtatc
     5581 ccagaacggt aaagatgtcg tttgccccat cgctctgcaa aacccggccc agagcagaaa
     5641 acgcccaggt tttgaacttg ttcgagaagc tgccctcgag aacgagcttg accttctcgc
     5701 tcgaatcgag actcttggtc tttgtcttcg aggtgcgcag gccatgctct ttcagcaggc
     5761 gcacgacctg ttgaacgctg ccgtcgatct gttcttcgct gtcgctgaaa aactggtgca
     5821 tctcgtcgag cagatcgggg cgttcgcgca tcagatactc agcgatgacg cgaatgccga
     5881 cgacggtcgg caggaatttg ctggagatgg tcttgatttc ggctgcggac atgcggggaa
     5941 gctccggctt attttcctgt tctttgatca tcgccgcttc gagcaggcga atgggaatgg
     6001 acaggtgttt gcgcttcaga gactcgcggt tgcgatgata catgagcaca tcgagacaca
     6061 actcgttgat ctggtgattg attcccgcgc ttttttcgac ggggattgtc accttgttat
     6121 tggcatagcc atcttctgcg tgcgcggtcc agacctcgcc gaagacgtgt ccggcccagc
     6181 cgagagccat gaccacgtca gcctgaccga atttcgggtc ttgcttggcc agctcgatca
     6241 tacgcacgac ggtatccggg tcgccgtgaa agtcacgtgg aaaagaggct ccaccctgag
     6301 atttgatgtc cgggcaggga ccaccgccga tgaagtcgtc ggcgagttca cggggaatcg
     6361 ccagctgatt cccgatctcg cgcacggcct gttcatacgc gcgctggttg accgtcaggt
     6421 gggtcatggg cccccaggcg tctgcggtga tcggtaaaac gagcagcaga gccagaagaa
     6481 acattccggc acgctgaatg atgctgttca tgcgaatacc tccgatgggc ggcgtgtgta
     6541 ccgcaccatg ttttctcatt ataagaaaaa gtccctctcc ccggaagata catcttgacg
     6601 gagaagagaa aatcacgcac catgacccga aaggcctgtc atttcacaga tcaacccgcg
     6661 aaaggaaacg gacatgaaga aagcttctgc caagagtgcg accccgaaac ccaaaaaaga
     6721 ggggccgcgc agttcagctc acggagtttg ctggaacctt ggtgacctgt acaaagatct
     6781 caaagacaaa cagatcgagt cacactacca gacggctttg aaacgcgcgc gcgaattcga
     6841 gaaaaaatat aagggaaaat tggctccgaa accgaagttg agcgggaaac agctcggaca
     6901 gatggttcag gaatacgaag aaattctcat gctgcgtgat cgtccgcaca tctacgcgca
     6961 cctgttgttc gcatcggaca cccagaaccc ggattacgga cgcaaactgc gcgagatgca
     7021 gaatcgttcg accgaaatca ccaaacacct gatcttcgtc gatctcgagt ggtgcgcagt
     7081 ggcggacgcc gacgcgaaga agctgctggc tgacgccgcg gtgaaaaaat accgtcacta
     7141 tctggaagcc tcgcggcggt acaagccgca tctgctgagc gaagccgaag aaaaagtcat
     7201 cgacgagatc gagaataccg gttcgcgagc ctttacccgc ctgtttgacg aactgctggg
     7261 cgccatgaac ttcaccgtga aggtgaaagg tgaaacccgc accatgagcg agcaggaggc
     7321 tctttcgctc ttgtacaatc cggatcgcga tacccgacag gctgcggctc aagcgctgac
     7381 cgaagggttc aaggcagggc tcaagccgat cacgttcatc ttcaatactc ttgtcgatca
     7441 ccataccgtc gtcgaccggt tgcggtctta cgccgacccc atggcctcgc gcaatctttc
     7501 caacgacatc gaacccaaga ccgtcgatgc gctgctcaag acctgcgatg cgaacaccgg
     7561 gttggtaagt cgctattaca agctcaaggc caagcttctc ggcctcaaga agttgtacga
     7621 ttacgatcgg tacgcgccca tttccgccga catgccttcc tgctcgtggg ccgaaagcaa
     7681 agagttgatc atcgatgcct accgcgagtt ctcacccgaa ctggggcgca tcgtcgaaga
     7741 gttctaccga aaaaactgga tcgacgccga gttgcgcccc ggcaagcgtg gtggtgcttt
     7801 ctgcgccggc acgacgcccg atgtgcaccc gtacattctc accaactaca gcgacaagct
     7861 gcgcgatgcc gtgaccatgg ctcatgaact cggacacggg gtgcatcagt atctctcgcg
     7921 caaacagggg tatctgcaga gtgacactcc gctgaccctg gctgagacgg ccagcgtgtt
     7981 tggcgaaatg ctgacgttcc atcgtctgat gggcaggcag accaatccgg cg
//
LOCUS       DAORHD010000038         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8802_length_7893_cov_4.060092, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000038 DAORHD010000000
VERSION     DAORHD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8802_length_7893_cov_4.060092"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKO06_01575"
     CDS             complement(<1..426)
                     /locus_tag="PKO06_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HNV68358.1"
                     /translation="MRNRRRNPRRGQALVEMAILFPFFLLIVVGGIIDFGFAFYNILT
                     LQQITDDVCMRLAENSRYSSPTSNTPIVPTDGSTQAFSLKPSWWAGDFQPSISPAAPV
                     PGFANHHYIRVSITYQSQTYTPFYQVILSSTAAVYGIPAR"
     gene            complement(605..1144)
                     /locus_tag="PKO06_01580"
     CDS             complement(605..1144)
                     /locus_tag="PKO06_01580"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNV68359.1"
                     /translation="MDLSSLTPDQALKLAAVVGATIACATDMTTGKIKNWLTFPMMVG
                     GLVANTWFFGWSGLGWSIVGLITGILVYLPLAVFGFVGMGDVKLLSGIGSVAGPGFMF
                     LTLLYGSVAAIPHALVIQWLNYGKNALQMFATSFTSGSWKKKTIHDDNATVRYHFYMG
                     LSLWIGSLAAWYFPLNIRW"
     gene            complement(1244..3193)
                     /locus_tag="PKO06_01585"
     CDS             complement(1244..3193)
                     /locus_tag="PKO06_01585"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNV68360.1"
                     /translation="MAQETLGEGIWRSLSANRRKALLIIGAAIALTTFFHYQSPVTYE
                     SAALLRVLSSDAARQENLAASMNGILAQDGVLRDLMTRAGLTPEAIDPKDRIRLEDAG
                     NGLVKLIVRHESSAVLQTLSNAAVQMLSDHYLGFNAEEDSFALESLRRKKMALQEKLD
                     QSKLELASLEQQTANPEAAVAPAERELQLLTLDLESDRRRLAEMPRVRKVSVQEQSPE
                     YADARARFNAAKGQLSELLKNYREKHPRVVEAMNELSSAEARLKQASRARTREEPNPE
                     IAECEKVIAEKEILLEQARHQAQAAESERLTRLDEAQRKRQETEYRVQALTTLFNETV
                     RQLEGLEIARHTAAGKIQVLHRTGDRPAPTGLSLIQREILGLLSGSLLAVVLLYSPSP
                     RPHPLITVPDAAILAGGGLPYTTETEIVHRILQVPSLAQSRLALPMPSDNSAQEHDER
                     LIALNEPHSRKLEPFKGLRTNLQILIAETRTRIVLVSSSRAGMGRTTLVANLGTLFAQ
                     DGYSVVLVDANLRKPALHRVFDLDNHAGLTSTLSSQMNPPILPYPGVSHLSILPSGPL
                     PPNPAELLGSRPMIELLDLLKRRYDLVLIDTPPLLEFPDAGILASITGGVIYLSRQGE
                     SEQDVTSCRDFLKSTRAKILGFVQT"
     gene            3311..4678
                     /locus_tag="PKO06_01590"
     CDS             3311..4678
                     /locus_tag="PKO06_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68361.1"
                     /translation="MDGRVSPHSKSGSAGNPGSPKPGPTIYRLPVITFASTLHDAHAV
                     ESLTTRLMSNLVGDGLEPAAVKALEEAGVETPAFLILTGGTENQALSVMLQRSGPFLL
                     LAHPEHNSLPAAIEILAGLRQNGRRGRIVLIHNPHAARVKIRRFLRLAAAHQSLRTMR
                     LGLVGRPSDWLAASMVDPRWVSRRWGPEVVLIDMDELLGRLEHLDEAEVRSLATEMSR
                     GVAEVREPTEADLTESGRFYLVLRDIARAQKLAGLSLRCFDLVQKFRQTGCLALSRLT
                     DEGCVGGCEGDLPALLTMAWLRAVSGEAPFMANPQDMDPEQNQLWLAHCTIARSWLSR
                     CVLRSHFESGVGVGIQGTIDPGPITIARIGGRRLDEVMMFDGHLVGGEALETRCRTQI
                     RVSLKGRVQEFLERPLGNHQVLVRGAWRDDLDEYLSLFMSTAGESPTDRSPDLPETGP
                     ISKYT"
     gene            complement(4749..5852)
                     /locus_tag="PKO06_01595"
     CDS             complement(4749..5852)
                     /locus_tag="PKO06_01595"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV68362.1"
                     /translation="MKVPLLDLKPQYGQFKDKLVPEILAIMESQMFILGPKVEKMEKE
                     LADYIGAKHALGVSSGTDAILIALMALDLKPGDEIITTPYTFFATAGCIHRAGAKAVF
                     VDIEPDTFNIDPKKIEKAISKKTRAIMPVHLFGQCADMDAINAVAKAHNLPVIEDCAQ
                     AIGSKNKGRQAGNMSLAGCFSFFPSKNLGCFGDGGLITTNDSAYFDRIKRLRMHGQSG
                     TYIHTEVGINGRLDALQAAVISAKLPLLAGWSDGRRRNAARYAELFKKIPQVKPPVAR
                     AENEHIYNQYVIRVPKRDDLKAHLTKHEIGCAVYYPLSLHEQECFKYLGYKKGDFPVS
                     EEAAQCTLALPVFPELVPEQIDFVGKTIAEFYK"
     gene            5942..7231
                     /gene="obgE"
                     /locus_tag="PKO06_01600"
     CDS             5942..7231
                     /gene="obgE"
                     /locus_tag="PKO06_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013859799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNV68363.1"
                     /translation="MKFVDELVIQVSSGKGGDGMVAFRREKFVPFGGPSGGDGGRGGD
                     VILEATRELNTLYDLSFRREYEADSGGKGGPKDKTGFDAAPLTVLVPAGTLVYDEETG
                     ELLCDLDAVGKKFLAAKGGRGGRGNAKFVSSVRRAPRFAEKGEPLQTRRLRLALKLLA
                     DVGLVGLPNAGKSTLLSVVSRARPKIADYPFTTLVPELGIVKIDGLPSFCMADLPGLI
                     EGAHEGAGLGIRFLKHTERTRALVHVIDVSAIDPEEPLASFSTVMKELKAFSKDLAKK
                     PQIVAANKMDLPDAREAWGTLQKALKRKKIKVFPISGATHEGLDDLVRGVAQMLKELP
                     AVEPAVVALPRVERRTDVAPSFRIDAVRPHLWEVTGNQIERWVEMTDFTNEESVRRFK
                     KRLKTCGFIEALADIAADLTDTVVISSMEFEYREFFA"
     gene            7228..>7893
                     /gene="nadD"
                     /locus_tag="PKO06_01605"
     CDS             7228..>7893
                     /gene="nadD"
                     /locus_tag="PKO06_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNV68364.1"
                     /translation="MTKVGLMGGSFNPVHRQHLAIARRVRAALGLDEVRLIPVFQPVH
                     KPGEEMLPFGSRVELLRAAIGVERGLVVDDVEQRLSGLSYTIRTIRYLKEQNPYAQFM
                     LIIGSDSLHDLPNWRESESLIREVPLCVCLRPGFPIRGPLPGTASVQVEIDESPVCSR
                     RIRQSLSRGEFAGLELAAPVVARLVEQDSYGCLGEPHRSWVLRLQALIDEMDPGMREH
                     IRSV"
BASE COUNT         1526 a         2191 c         2599 g         1577 t
ORIGIN      
        1 ccgcgccggg atgccgtaga ccgcggccgt cgaactcagg atgacctggt aaaacggggt
       61 gtatgtctga gattgatacg tgatcgaaac acggatgtag tggtggtttg cgaatcctgg
      121 aactggggcc gccggtgaga tggagggttg gaaatcacct gcccaccatg aaggcttcaa
      181 actgaaggct tgcgtactgc cgtcggtagg aacaataggg gtgttcgaag ttggagagga
      241 atacctgctg ttctcggcca ggcgcatgca cacgtcgtcg gtaatctgct gaagcgtcag
      301 aatgttgtag aacgcaaagc cgaagtcgat gatgccaccg acgacgatca gcaggaaaaa
      361 ggggaacagg atggccattt cgaccaatgc ctgaccacgt cgcgggttac ggcgccgatt
      421 tctcatacgt taacttcctc ccttggtaca ctagagctag caagaaacgt gctcgccgtc
      481 gcgggagcca ttcgccccgg cggtctctct gcacagatat ctactctatc aaatttaatt
      541 cgccgtgaat agcgttgaaa ggaaaaacta cacgaaagct gtggaaaaat tccgcgaacg
      601 gtattcacca ccgaatgttc agcggaaaat accaggccgc cagactcccg atccagaggc
      661 ttaaccccat gtagaaatgg tagcggaccg tggcgttgtc gtcgtgaatg gtttttttct
      721 tccaggagcc gctggtgaag ctggtagcga acatctgtag ggcatttttg ccgtagttca
      781 accactgaat caccaacgca tgaggtattg ctgccacaga cccgtacagc aacgtgagga
      841 acatgaatcc cgggcctgcg accgagccga taccgctcaa gagcttcaca tcgcccatgc
      901 cgacaaaacc gaacacggcc agtggcaggt agaccagaat gccggttatc agaccgacaa
      961 tgctccaccc gagcccagac cagccgaaga accacgtgtt ggcgacgagc ccccccacca
     1021 tcatcggaaa ggtcagccag ttcttgatct tgccggtggt catgtcggtg gcgcaggcta
     1081 tggtcgcgcc gacgacggcg gcgagtttca gcgcctgatc aggggtaaga ctcgagaggt
     1141 ccatcggtga gagtgtagca aaaaccgacc cagacgtacc tacttttttc ccgcaaggca
     1201 aaaagccgcc cgaaggcggc tcatgacgcg ggagaaacgg cgctcaggtc tgaacgaacc
     1261 cgagaatctt ggcgcgtgtg ctcttcaaga agtcgcgaca cgaggtgaca tcctgctctg
     1321 attcgccttg gcgagacagg tagatgacgc caccggtaat cgaggccaga atgccggcat
     1381 cggggaactc gagcagcggc ggtgtgtcga tgagcaccag atcgtagcgg cgcttgagca
     1441 gatcgagcag ttcgatcatc ggccgtgagc ccagaagttc agcggggttt ggcggcagcg
     1501 gcccggaagg cagaatcgag aggtgcgaca cgcccggata cggcagaatc ggcgggttca
     1561 tctggctcga caacgtcgag gtcagacccg cgtggttgtc gagatcgaac acgcgatgca
     1621 gagccggttt gcgcaggttc gcgtcgacga gcacgacgga atagccatcc tgagcgaaga
     1681 gcgtgccaag attggcgacc agagtcgttc gccccatgcc ggcccgggag gaactgacca
     1741 gcacgatgcg ggtgcgggtt tcggcgatga gaatctgcag attggtgcgc agccctttga
     1801 acggttcgag cttgcgggaa tggggctcgt tgagggcgat gagccgctcg tcatgctctt
     1861 gcgcggaatt gtctgaaggc atcggcaggg cgagtcgact ttgcgccagc gagggaacct
     1921 gcagaatgcg gtgaacgatc tcggtttcgg tggtatacgg caacccgccg cccgccagaa
     1981 tcgccgcatc gggcacggtg atgagcgggt gcggacgtgg agaaggcgag tagagcagaa
     2041 cgacggcgag cagcgagccg ctcaacaggc cgagaatttc gcgctgaatc agagagaggc
     2101 cggtgggggc ggggcggtca ccggtgcgat gcagcacctg aatcttgccc gcggcggtat
     2161 ggcgtgcgat ttcgagccct tcgagctggc ggaccgtttc attgaagagc gtcgtcagag
     2221 cttgaacacg atactccgtc tcctgtcgct tgcgctgggc ctcgtcgaga cgggtgagtc
     2281 gctcggactc agcggcttgt gcctgatggc gcgcctgttc gagcagaatc tctttctcgg
     2341 cgatgacctt ttcgcattcg gcgatctcgg ggttgggctc ttcgcgcgtg cgggctcgag
     2401 acgcctgctt gagccgcgct tccgctgacg agagttcgtt catggcttcg acgacgcgcg
     2461 ggtgtttctc gcgatagttc ttgagcaact cggacagttg cccctttgcg gcgttgaacc
     2521 gggcgcgggc atcggcatac tcgggtgact gttcctgaac cgaaaccttg cgcacgcgag
     2581 gcatttcggc cagtcggcgc cggtcggatt cgagatcgag ggtcagcagc tggagttccc
     2641 gttctgccgg ggcaacggcg gcttcggggt tggcggtctg ctgttcgagc gaagcgagtt
     2701 cgagtttcga ctggtccagt ttttcctgca acgccatctt cttccgtcgc agactctcaa
     2761 gcgcgaagga atcttcttcc gcgttgaagc cgaggtaatg gtcggaaagc atctgtaccg
     2821 cggcattgct caaggtctga agcacggcgg aagactcatg gcgcacaatc agctttacca
     2881 ggccgttccc ggcatcttcg agtcgaatgc gatctttcgg gtctatcgct tcgggggtga
     2941 gaccggctcg ggtcatcagg tcgcgcagaa cgccatcctg cgcgagaatg ccgttcatcg
     3001 aggcggccag gttttcctgg cgcgcggcat cagacgaaag gacgcgcaac agggcggcac
     3061 tctcgtaggt aaccgggctc tgataatgaa aaaaggtggt cagggcgatg gccgccccga
     3121 tgatcagcag agctttgcgg cgattggcgg aaaggcttct ccagatgcct tcacccagag
     3181 tttcctgagc cataggtgcc tcctggaaca aacaagatct tccttggtat tatcggcaga
     3241 gcacctgttt gatctgagcg attcgcaaga cagggttggt cagacatttt tgttctacag
     3301 gaggacgcgt atggacgggc gagtctcccc acattcgaag agcgggtctg ccggaaaccc
     3361 gggttcaccg aaaccgggcc cgacgatcta tcggttgccg gtaatcacct ttgcgtcaac
     3421 gcttcacgat gcccatgcgg tcgagtccct gacgacacgc ttgatgtcga atctggtcgg
     3481 ggacggcctc gagcccgccg ccgtgaaagc actcgaagaa gccggggtgg agacccctgc
     3541 ctttctgatt ctgaccggtg gcacggaaaa tcaggcgttg tccgtcatgt tgcagcgatc
     3601 gggcccgttt ctgctgctgg cccacccgga acacaactcg ctgcccgcgg cgatcgagat
     3661 tctcgccggc ctgcgtcaga acggtcgaag agggcgcatc gtgctgattc acaaccccca
     3721 tgcggcgcgg gtgaagattc gccgcttctt gcgtctggcc gcggcgcacc agtcattgag
     3781 gacgatgcga ctcggcctgg tgggacggcc ttcagactgg ctggcagcct cgatggtcga
     3841 tccgcgctgg gtcagccggc gctggggccc ggaggtggtg ctgatcgaca tggatgagct
     3901 gctcgggcga ctcgagcacc tcgatgaagc cgaggtgcgg agtctggcaa ccgagatgtc
     3961 acgcggggtg gccgaggtgc gtgagccgac ggaagccgat ttgacggaat cagggcggtt
     4021 ttatctggtt ctgcgcgaca tcgcccgggc ccagaagctg gcgggcctct cgctgcgctg
     4081 tttcgacctg gtgcagaagt ttcgccagac cggttgtctg gcattgtcgc gactgaccga
     4141 tgaagggtgt gtcggtggct gcgagggcga tctgccggcg ctgctgacga tggcctggct
     4201 gcgggcggtc agcggcgaag cgcctttcat ggcgaatccc caggatatgg acccggagca
     4261 gaatcagctc tggctggccc attgcacgat tgctcgaagc tggctgagcc ggtgcgtgct
     4321 gcgctcacat ttcgaatcgg gcgtcggggt cggaattcag ggaacgattg atccggggcc
     4381 gatcaccatc gcacggatcg gcggccgtcg actcgatgag gtgatgatgt tcgatggaca
     4441 tctggtcggc ggagaggcac tcgaaacccg ctgtcgaacg cagattcgcg tttcactgaa
     4501 ggggcgggtg caggagtttc tcgaacgacc gctggggaac catcaggtgc tggtgcgcgg
     4561 agcctggcgc gacgatctcg acgagtatct gagcctcttc atgtcgaccg cgggcgaatc
     4621 gccgactgac cgttcgccag atctgcccga gaccggaccg atttcgaaat atacctgaga
     4681 gatatacctg agaaaaacag caccggccgc catttctggc ggccggtggc aggtacacaa
     4741 cgaacggact atttgtagaa ctcggcgatc gtcttgccga cgaaatcaat ctgctccggc
     4801 accagctcag ggaacaccgg cagggccaga gtgcactggg cggcttcttc cgacacgggg
     4861 aaatcaccct ttttatagcc caggtatttg aagcattcct gttcgtgcaa cgagagcgga
     4921 tagtacacgg cgcacccgat ctcgtgcttg gtgagatgag ccttcaggtc gtcgcgcttc
     4981 gggacacgaa tgacatactg gttgtagata tgctcgttct cggcgcgggc aaccggcggc
     5041 tttacctgcg gaatcttctt gaacagttcg gcatagcggg cggcgttgcg acggcggccg
     5101 tcgctccagc cggcgagcag aggcagcttc gccgagatga ccgcggcctg cagagcgtcg
     5161 agacggccgt tgatgccgac ttcggtgtga atgtaagtgc cggactggcc gtgcatgcgc
     5221 aggcgtttga tgcgatcgaa gtaggcggag tcattggtgg tgatcaagcc gccgtcgccg
     5281 aaacatccga ggttcttgct cgggaagaag ctgaaacacc cggcgaggct catgttgccg
     5341 gcctgccgac ccttgttctt cgaaccgatc gcctgggcac agtcttcgat gacgggaagg
     5401 ttgtgcgctt tggcgactgc gttgatggca tccatgtcgg cgcattggcc gaacaggtgc
     5461 accggcatga tggcgcgggt ctttttggag atcgccttct cgatcttctt gggatcgatg
     5521 ttgaaggtgt caggctcgat atcgacgaac accgctttgg ctccggcgcg atgaatgcag
     5581 ccggcggtcg cgaagaaggt atacggcgtg gtgatgatct cgtcgcccgg tttgagatcg
     5641 agcgccatga gtgcgatgag aatggcgtcg gtgcctgaag aaacccccag ggcatgcttt
     5701 gcgccgatgt agtcggccag ttccttttcc atcttttcga ctttggggcc gaggatgaac
     5761 atctggcttt ccatgatcgc gagaatctca gggacgagct tgtctttgaa ctggccatac
     5821 tgaggcttca gatcgagcag agggactttc atgcgggact cctgtcttca gaggtttgca
     5881 cccgaaagct atcaccttcc tcgctgccgg tcaatcgtca tctgtggtat actgcgcgac
     5941 tatgaaattc gttgacgaac tcgtgattca ggtgtcttcc ggcaagggtg gcgacggcat
     6001 ggtggccttt cggcgcgaaa agttcgtgcc gttcggcggg ccgtcgggtg gcgacggcgg
     6061 gcgtggcggc gacgtcattc tcgaagcgac gcgcgagctg aacacgctct acgacctcag
     6121 cttccgccgt gagtacgagg ccgattcggg tggcaagggc ggcccgaaag acaagaccgg
     6181 gttcgatgcc gcaccgctga cggtgttggt gccggctggc acgctggtct acgacgaaga
     6241 gaccggtgaa ctgctgtgcg atctcgacgc ggtcggcaag aagtttctgg ccgccaaggg
     6301 cggtcgcggg gggcgcggca atgccaagtt cgtctcgtcc gtgcgccggg caccgcgctt
     6361 tgctgaaaag ggcgagccgc tgcagacccg ccggctgcgt ctggccctga agctgctggc
     6421 cgatgtcggc ctggtcgggt tgccgaacgc tggtaagtca acgcttctga gcgtggtcag
     6481 ccgggcacgg ccgaagattg ccgactatcc gttcacgact ctggtgcccg aactcggcat
     6541 cgtcaaaatc gacgggttgc cgtcgttctg catggccgat ctgcccgggc tcatcgaagg
     6601 ggcgcacgag ggcgcgggtc tgggcattcg ctttctcaag cataccgagc gcacgcgggc
     6661 gctggtacat gtgatcgatg tctcggccat cgacccggaa gagcctctgg cttcattctc
     6721 cacggtcatg aaggaactga aagccttctc gaaagatctg gcgaaaaaac cgcagatcgt
     6781 ggccgccaac aaaatggatc tgccggatgc ccgcgaagcc tggggaacgc tgcagaaagc
     6841 gctcaaacgc aagaagatca aggtgtttcc catttccggg gccacgcacg aaggtctcga
     6901 cgacctggtg cgcggggtcg cgcagatgct gaaagagctg ccggcggtcg aacctgcggt
     6961 cgtggcgttg cctcgcgtgg agcggcgtac cgacgtcgcg ccgtcgttcc ggatcgatgc
     7021 ggtgcggcct cacctgtggg aagtgacggg aaaccagatc gagcgctggg tcgagatgac
     7081 ggacttcacc aatgaggaat cggtgcgacg gttcaagaag cggctgaaaa cctgcgggtt
     7141 catcgaagct ctggccgaca ttgccgccga cctgacggac accgtggtca tcagctcgat
     7201 ggagtttgaa taccgggaat tctttgcatg acgaaggtgg ggctcatggg cgggagtttc
     7261 aaccccgttc atcgtcagca tctggcgatc gcccgccgcg tgcgcgccgc gcttggtctc
     7321 gacgaggtgc gcctgattcc ggtgtttcag ccggtgcaca aaccgggcga agagatgctg
     7381 ccgttcggca gtcgcgtgga attgctgcgg gcagccattg gcgtggagcg cgggctcgtg
     7441 gtcgatgacg tcgaacaacg gctttccggg ctgtcctaca cgattcgtac cattcgctat
     7501 ctgaaagaac agaatccgta tgcgcagttc atgctgatca tcggatcaga ttcgctgcac
     7561 gatctgccga actggcgaga gtccgagtcg ctgattcgcg aagtgcctct gtgcgtgtgt
     7621 ctgcggccag ggttcccgat tcgtgggccg ttgcccggca cggcgagcgt ccaggttgag
     7681 atcgacgagt cgcctgtctg ttcgcggcga atccggcagt cgctttcacg cggggagttc
     7741 gccggcctgg aactggcggc gccggtagtg gcccgcctgg tcgaacagga ttcctacggt
     7801 tgcctcggag agccgcatcg gtcgtgggtg ctgcgattgc aggcgctcat cgacgagatg
     7861 gacccgggga tgcgggagca cattcgctcg gtg
//
LOCUS       DAORHD010000039         7847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_8876_length_7847_cov_3.998460, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000039 DAORHD010000000
VERSION     DAORHD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8876_length_7847_cov_3.998460"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKO06_01610"
     CDS             complement(<1..1177)
                     /locus_tag="PKO06_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68365.1"
                     /translation="MTFHGSPLSNRFLRARIRAGFILPVAMLLISILMILGWMFLSTT
                     MHSKNISNVFYRDDMARLVAESAAAELRVRFAELLEKNAALQAMCSNPTGPGSTMTLS
                     QSEFPQTTALADTLAGSGQIDFSCMLSTTHVDTDLKEGAVAVRFDGEYQATLQMTFTV
                     ALGTGTKQRNSQFVYEHDLKRVNFRSSSVGRTGRGYTSTMANDYVLYVRNALGEFDKF
                     NGLSLNNNERGLAVLQPEAGKKGRLFFGTARPTDSQQEKKYVFINIDKSLEALIPPAP
                     PVTGVSWSDMQSQMPIFAGKIQAVIDEAKSKSEGKINFYPDRIKSELSVEYKPIVKEN
                     NIWWQSLWNKIKEVFNSFIAKVTGKQKNVDKGIGLLGENNSDFSICSLIDGNLRQRFW
                     "
     gene            complement(1341..2021)
                     /locus_tag="PKO06_01615"
     CDS             complement(1341..2021)
                     /locus_tag="PKO06_01615"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HNV68366.1"
                     /translation="MKTSVSFSLPPGPLRLGVIEADGVQVGPSTPPYLNQIRDDVRPM
                     LSESYVYPDHLQKGIRSLLKAYGFHPSGRNRPASEFLVKDLQGRGGFKSINNVVDINN
                     HLSLLSHLPISILDREKTGDHLCIRIGTETEKYIFNQEGQDLSLKHLLIVARGDGDLA
                     PFGSPVKDSHATKISVTTHNLVGFIYATPTTASIEEMQHWLKLFSDLLRSEGKASDIA
                     WEILDGPQ"
     gene            complement(2365..3723)
                     /gene="murC"
                     /locus_tag="PKO06_01620"
     CDS             complement(2365..3723)
                     /gene="murC"
                     /locus_tag="PKO06_01620"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNV68367.1"
                     /translation="MNAFFIGIGGMGMSGIAKILKQTGHHVAGSDRNLEGDYCRRLKA
                     LGVDLYLQDGTGLEAFLAAHSLQPAEIVVVKSTAVEDQVPDVIAARRLGIREMMRSDL
                     LAQLFHEKQGIAIGGSAGKTTTTGLVTWLLKFAGFEPTSAVGGIVSGLETNALAGTGP
                     HFVIEADESDGSIVKYHSHIALLTNISRDHKTMDELMELFRTFLSNVHPDGTVLVCSD
                     DAQNLELMKTLTRPVVTYGIGPEATIRAENIVIGQDQITFTVAGCPFTLLLPGMHNVQ
                     NALGAIAIGMRLNIPLEEIAAALLAFPGMKRRFERVGTARGVTVVDDFAHNPAEIQAA
                     IKTARHASRRRFIVYQPHGFGPTRFTRPDLVKVFSELNPEETLYLDDIFYGGGTVEKD
                     ISSRDIIEEVRHRNPNAFYLGDRRQIVERIAKEARQGDMVLVMGARDINTICRQILDY
                     LQ"
     gene            complement(3825..4457)
                     /locus_tag="PKO06_01625"
     CDS             complement(3825..4457)
                     /locus_tag="PKO06_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV68368.1"
                     /translation="MPTLRPLSRLVLLLVILSLIPGCGSRIHEDSDNSALVQGRLPVY
                     QIGDQVLLKNDLRTIELPVDTNGSFAGKVLPGTYQALIRSKSGTLKLIEMSLTVEDNL
                     TINLLNASLVPIPRVISVSVPLVYEDSAVVEWETDIDSDGRVDYGVDTAYGYSSFTDP
                     ELTTRHRIQLYGLSPNTSYHFRVVASRHSLESVQTFSPDYVFVTENRMEP"
     gene            complement(4478..6154)
                     /locus_tag="PKO06_01630"
     CDS             complement(4478..6154)
                     /locus_tag="PKO06_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV68369.1"
                     /translation="MRLLHRFTSRLGLLILLLLGSQAHTVLAAPAVPGNLTFAALARH
                     LYLPGQAALIFVDGPVYFHSLATKKWKAAALNQILLDSDSIRTGPHGYAAISFSTDNL
                     LLLHPNSGVRIKLRESGSPRLTVQVHKGEMLLSVVDSNDVVVEGRQAHLFLQRGEFSL
                     RSTEKEETVRALLESSFVRLTGGVTPLKLPAGYQLTIDAQGRESKPQRFDTIQEYATF
                     RRFRTALENFRGTNAQLSTDIAFRIDSVLVDDIFVSNLSTNEHGYRVINPGSKPAPRT
                     LLFRMKVTPFPRPQDRFEIAITKDLIFPLKEAGNGFLEARIPTPGFPEFHLKVHYVDE
                     NDRRAKLYESNFVLSNRRQKIDEIKAFLTELTRRFERRDFLFFREQVSRDYRDWFGNS
                     YFDFIKSLEDSLRNYRDIRLVLRPHSFTFKGDEVLVDLTYRLTALTSGWKMRYDDLGS
                     DLLTLVYRDGSWKLKGKTRGLFLQRLRVANDLRLGILRGQVKDEATGSPVRGAQVRIL
                     RTRYSTTTDSMGEYLIHNIAPGTYDIEITKNGFGRLLITQMEIKPSGDRF"
     gene            complement(6154..>7847)
                     /locus_tag="PKO06_01635"
     CDS             complement(6154..>7847)
                     /locus_tag="PKO06_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68370.1"
                     /translation="NPRYLPEFSGKDPTALSEPPFVPPPTMDLGSHIVQTRPASQMRP
                     PEVSTSLALGDDRELQLRNLNESEDEKLYTSQLVTDTTLYGTMVDGARNLSSRSQSTY
                     YEQNIRYDLYSTRLDGHYLSLQLDSTYCNDDREYADAFTLNQLSLESKTPHSRLVFGH
                     AYPEFSRFSMSQQMVGLYGTQKFSHTEAKAFTGYRSITNDALRNPRLVSGFRLQHDSD
                     ESFTMGLNAVGTRDRHESPGSSDDLPTLRNKVYSFDCLLKPNENWSFEAEVARSDTDF
                     DQRSEAGNQYANAYRAQGTWTRENAQLQGGIEQADTAFLTMLGESLRDQRAYYGRFFY
                     ELNEYVSGRVATRLTRDNLDNYKRATLIRNEPEVQLTFKPSEYYRDLRFEFFYQPLHE
                     YSDNGDFLDRYRDLTWLEMHQKAGAMRYYTGLAWTIDKDKIDRTNDRDTTKFDLKATW
                     EYDAHRQLYGLLGIEQYALKTVGGKDITKTIGLGGRSLFHEDITLDLDYLHEISDVKA
                     RQLDSIHDRLTFSVTKEYNNYARFIVSVEGTRHQFDMSDQDYSDVAAKVRLLRFF"
BASE COUNT         1688 a         2035 c         2357 g         1767 t
ORIGIN      
        1 gccagaagcg ctggcgaaga ttgccatcga tcagactgca gatggaaaag tcgctgttgt
       61 tctcgcccag caggccgatg cccttgtcga cgttcttctg ttttccggtg actttggcga
      121 tgaaactgtt gaatacttct ttaatcttat tccacagaga ttgccaccag atgttgtttt
      181 ccttaacaat tggcttgtac tctacagaga gttccgattt gatgcgatca gggtagaagt
      241 tgatcttccc ttctgatttg ctcttggctt catcaataac ggcctgaatc ttgccggcaa
      301 aaatgggcat ctgagactgc atatcactcc aggaaacacc ggtcaccggc ggagcgggag
      361 gaatcagggc ttcgagagat ttgtcgatgt tgatgaatac gtactttttc tcctgctgcg
      421 aatcggtggg gcgggcggtg ccgaagaaga ggcgtccctt cttgcccgct tcgggctgta
      481 ggacagccaa accgcgttcg ttgttgttca gtgacaaccc gttaaacttg tcaaactctc
      541 cgagggcgtt gcgaacatac aacacgtagt cattcgccat ggtcgaggtg tagccgcgcc
      601 cggtgcgccc gacagaggaa ctgcgaaaat tcacgcgctt caggtcgtgc tcatacacga
      661 attgggagtt tcgctgtttt gtgcccgtgc cgagggcaac ggtgaaggtc atctgcagag
      721 tcgcctggta ctctccgtca aagcgcacgg cgaccgcacc ctctttcaga tccgtgtcga
      781 cgtgggttgt gctcagcatg caggaaaaat cgatctgtcc agaccccgcg agcgtgtctg
      841 ccagagcggt tgtttgggga aactcgctct gactcagcgt catcgtgcta cccggcccgg
      901 tgggattact acacatggct tgcagagccg cattcttttc caacaactcg gcgaaacgaa
      961 ctcgcagttc tgcggcagcg ctctcggcca ccaggcgagc catgtcgtcg cggtaaaaaa
     1021 cgttgctgat gttcttggaa tgcatggtgg tggagaggaa catccaccca agaatcatca
     1081 gaatcgagat caataacatg gccaccggca gaatgaagcc ggcacggatt ctcgcccgga
     1141 gaaaacgatt ggacaaagga gagccatgaa atgtcataat cttctcctct attatacaac
     1201 cggttgggtt gacaacccaa ctttcctcta cgaggaaggg aattttctca cgaacagctg
     1261 caggagtaac ccgcctcatc gacttcggga atctggacca ggctggggat taagacgtgg
     1321 acgtggaggg tgcgaggccc tcactgggga ccgtcgagaa tctcccaggc gatatcagag
     1381 gccttccctt cactacgcag caggtcactg aaaagtttca gccagtgctg catttcttca
     1441 atggaagcag tggtgggcgt ggcatagatg aaaccgacca ggttgtgggt tgtgacactg
     1501 atcttcgttg cgtgagagtc tttcacggga ctgccgaatg gggcaagatc accgtcgccg
     1561 cgagcgacaa tcagcagatg tttcaggctc agatcctggc cttcctggtt gaaaatgtac
     1621 ttttcggtct cggtgcctat gcgtatgcac agatgatctc cggtcttctc acggtcgaga
     1681 atactgatcg gaagatgaga aagaagggac aggtggttgt tgatgtcgac cacgttgttg
     1741 atcgacttga aaccgccgcg cccctgcaga tctttgacca ggaactcgct ggccggccga
     1801 ttgcgtcccg aaggatgaaa accgtaggct ttcagaagac ttctgatgcc cttctgcagg
     1861 tgatccggat agacatagct ttctgacagc atgggacgaa catcgtcgcg aatctggttg
     1921 agatacggtg gcgtcgaagg cccgacctga acgccgtcag cttcaatgac tccgaggcgc
     1981 agtggtcccg gagggaggga aaacgaaacg ctggttttca tgacaggttc ccgaataatg
     2041 gcgtgtgttg tgttgcgaca ctttacacca aattgattcg gcggaaaagt acaacctgcg
     2101 caaagtcagg gtcgtttgat tctccggctt gggttttccc cttggcatcg ctcaacgatt
     2161 cgccgtcttg gcagggtgtg cgcccttcat ctcgaaatgc aggcagagag actgccgtgg
     2221 aacggcgaat ctcgctgacg ggaaaggttt gaataagtac aagctggcgt gaaacggaat
     2281 tctttgtggg actcgagtac gagggaataa aaaacggcag gtgcttacct gccgttggtc
     2341 tccgttgcgg atatcagaga ggggtcattg aagatagtcg agaatctgcc ggcagatagt
     2401 gttgatgtcg cgggctccca tgacgagaac catgtcgccc tgccgggctt ccttggcgat
     2461 gcgttcgacg atctggcgtc gatcgcccag atagaaggca ttcgggttcc gatggcgcac
     2521 ttcctcgatg atgtcacgtg aagaaatgtc tttttcgacg gtgccgccac cgtagaagat
     2581 gtcatcgaga tagagagtct cttcggggtt cagttcactg aagaccttga ccagatcagg
     2641 gcgtgtgaag cgggttggcc caaagccgtg cggctgatag acgataaacc ggcgtctgga
     2701 ggcatggcgc gccgtcttga tcgccgcctg aatctcggcc gggttatgcg cgaaatcatc
     2761 gacgaccgtg acgccgcgcg ccgtgcccac acgctcgaag cgccgcttca tgcctggaaa
     2821 cgccagcaac gcggcggcga tttcctcgag cggaatgttc aggcgcatcc cgatggcgat
     2881 ggctcccaga gcgttctgta cgttgtgcat gcctggcagc agcagcgtga acggacagcc
     2941 ggccaccgtg aacgtgatct gatcctggcc gataacaatg ttttcggctc tgatggtggc
     3001 ttcgggaccg attccgtacg taacgacagg acgtgtcagc gttttcatca gctccaggtt
     3061 ctgggcgtca tcgctgcaga cgagaaccgt gccatcgggg tgaacattgg agagaaacgt
     3121 gcggaacaac tccatcagtt catccatggt cttgtggtca cgggagatat tggtcagcag
     3181 ggcaatgtgg gaatggtact tcacgatgga cccgtctgat tcatcagctt caatgacgaa
     3241 gtgggggccg gtgccggcaa gagcattggt ctcgaggcct gagacgatgc cgccaacggc
     3301 gctggtgggt tcgaaccccg cgaatttcag cagccaggtg acgagcccag tcgtggttgt
     3361 tttacccgcc gagccgccga tggcaatgcc ctgcttttcg tggaaaagct gagccagaag
     3421 gtcggaacgc atcatctcgc ggattccgag gcggcgggcg gcgatcacgt cgggcacctg
     3481 gtcttcgacg gcggtcgact tgaccacgac gatctctgcc ggttgcagag agtgggcagc
     3541 cagaaacgct tcgaggccgg ttccgtcctg aagatacaga tcgacgccga gcgctttcag
     3601 tctgcggcag tagtcaccct cgaggttgcg atcagagccg gcgacgtggt gtccggtctg
     3661 cttcagaatt tttgcgatac cgctcatgcc catgccgccg ataccgatga aaaatgcgtt
     3721 catgaccagt acctccgaat cgtggaaaag aatgagattt cttacctaga ctatcggaac
     3781 aaggctctga aacgcttaga gcaggttgcg cccggcagag ggggtcacgg ctccatccgg
     3841 ttttcggtca cgaacacata gtcaggggaa aaggtctgca cactttcgag gctgtgccgg
     3901 ctggcgacga cgcggaagtg atacgaggtg ttgggagaca gtccatacaa ctggatgcga
     3961 tgccgtgttg tcagttcggg gtccgtgaat gaactgtagc cataggcggt atcgacaccg
     4021 tagtcgacac gtccgtccga gtcgatatcg gtttcccact cgaccacagc cgagtcttca
     4081 tagaccagcg gcaccgaaac gctgatcact ctcggaatgg gaaccagaga cgcattgagc
     4141 aggttgatgg tcagattgtc ttcgacggtc aggctcattt cgatcagctt cagtgtgccg
     4201 gacttcgacc ggatcaaggc ttgataggtt ccgggcagaa ccttgccggc gaaacttccg
     4261 ttggtgtcga ccggtagttc gatggtgcgc agatcgtttt tcagcagcac ctgatctccg
     4321 atctgataga cgggcaggcg cccctgtacc agcgcggaat tgtcggaatc ctcgtggatt
     4381 ctgctgccgc agccgggaat gagagaaagg atgacgagca ggagaacaag acggctgagc
     4441 ggtctgaggg taggcatggc ggtgttcctt gtcatgttca gaaacgatct ccgctgggtt
     4501 ttatctccat ctgcgtgatc aacaggcgtc cgaacccgtt tttcgtgatt tcgatatcgt
     4561 acgttccggg ggcaatgtta tgaatgagat actcgcccat gctatcggtc gtggtgctgt
     4621 accgtgttcg cagaattcgc acctgagcgc cgcgaacggg actgccggtc gcctcatcct
     4681 tgacctgacc gcgcagaatg ccgaggcgca ggtcgttggc cacccgaagt cgctgcagaa
     4741 acaacccgcg ggttttcccc ttgagtttcc agctgccgtc gcggtaaacc agcgtcagca
     4801 gatcactgcc gaggtcgtcg taacgcatct tccagccgct ggtcagagcg gtgagacggt
     4861 aggtcagatc caccagcact tcgtcgccct tgaaggtgaa ggaatgcggg cgcagaacca
     4921 gacgaatgtc tcgatagttg cgcagcgagt cttccagcga cttgatgaag tcgaagtaac
     4981 tgttaccgaa ccagtcacga tagtctcgcg agacctgttc gcgaaagaac agaaagtcac
     5041 gacgctcgaa tctcctggtt aactcggtca ggaaagcctt gatttcatca atcttttgtc
     5101 gccgattcga taggacaaag ttgctttcat acagtttggc gcgacgatcg ttttcgtcga
     5161 catagtgcac tttcaggtgg aattcgggaa agccgggggt cgggatgcgg gcctcgagaa
     5221 acccgttgcc agcctctttc agcggaaaga tgagatcctt ggtgatggcg atctcgaacc
     5281 tgtcttgtgg acggggaaac ggcgtcactt tcatgcggaa caggagggtg cgcggagcgg
     5341 gttttgaacc ggggttgatg acccggtagc cgtgctcatt ggtcgacaga ttgctgacga
     5401 agatgtcatc gaccagcact gaatcgatcc tgaaggcaat gtcggtcgac agttgggcat
     5461 tggttccgcg gaagttttcg agggccgtgc gaaaccggcg aaacgtggca tattcctgaa
     5521 tggtgtcgaa gcgctgaggc ttgctttcac ggccctgagc atcgattgtg agctggtaac
     5581 cggcgggcag cttcagtgga gtgaccccgc ccgtcaggcg gacaaacgat gattcgagaa
     5641 gggcacgcac ggtttcttcc ttctcggtcg atctcaaaga gaactcgccc cgctgcagaa
     5701 agaggtgcgc ctgtcgccct tcaacgacga catcgttcga atcaacgaca gaaagcagca
     5761 tttctccctt gtgaacctgg acggtcaaac gtggtgaacc gctctctctc agtttgatgc
     5821 gaacgcccga gttcgggtgc agcagcagca ggttgtcggt tgaaaacgag attgcggcgt
     5881 atccgtgagg acccgtgcgt atcgagtcgc tgtcgagaag aatctggttg agagcagcgg
     5941 ccttccactt cttcgtggcc agtgaatgaa agtacaccgg accatcgaca aagatcagcg
     6001 cggcctgccc tggcagatac aggtgtcggg cgagcgctgc aaaagtcagg ttgcctggca
     6061 cggcaggagc cgccaggacc gtgtgtgctt gactgccgag caacagcaga atgagcagtc
     6121 cgagacgaga ggtgaagcgg tgcagcagac gcattagaaa aacctcagaa gtcggacttt
     6181 cgcggcaacg tccgagtagt cctgatcgct catgtcaaac tggtgacggg ttccttcgac
     6241 actcacaatg aaacgggcgt aattgttgta ttccttggtg accgaaaacg tcaggcgatc
     6301 atgaatcgag tccagctgac gagccttgac atcgctgatt tcatgcagat agtcgagatc
     6361 gagcgtgata tcctcatgga acagtgaacg tccgcccagg ccgatggttt tcgtgatatc
     6421 cttgccgcca accgtcttca atgcatactg ttcgatgccg agcaacccgt acaattgccg
     6481 gtgagcgtcg tattcccagg ttgccttcag gtcgaatttc gtggtgtcgc gatcgttcgt
     6541 gcggtcgatc ttgtccttgt cgatggtcca ggccagtccg gtgtaatacc gcatggcacc
     6601 agccttctgg tgcatttcga gccaggtgag atcgcgatac cggtcgagga aatcgccgtt
     6661 gtctgaatac tcgtgcaacg gttgataaaa gaactcgaag cgcagatctc gatagtactc
     6721 gctcggtttg aacgtcagtt gcacttcagg ctcgttccgg atcagagtcg cgcgcttgta
     6781 gttgtccaga ttgtcgcggg tgagacgtgt ggcgacgcgg ccgctgacat actcgttcag
     6841 ttcatagaaa aagcgcccgt agtaggcacg ctggtcgcgc agactctcgc ccagcatggt
     6901 cagaaaggcc gtgtcagcct gttcgattcc cccctgaagt tgagcgtttt cacgtgtcca
     6961 ggttccctgg gctcgatagg cgttggcata ctgattgccg gcctcgctgc gctgatcgaa
     7021 gtcggtgtcc gagcgcgcaa cttccgcctc gaaactccag ttttcgtttg gcttcaacag
     7081 acagtcgaac gaatacacct tgttgcgcag agtcggcaga tcatcgctgc ttcccggact
     7141 ttcatgccgg tcacgagtgc caaccgcatt caatcccatg gtgaacgatt cgtccgagtc
     7201 gtgctgcagt cgaaagcctg acaccaggcg tgggttgcgc agcgcatcgt tcgtgatcga
     7261 acgatagccg gtgaaggctt tggcttccgt gtgggagaac ttctgcgtgc cgtagaggcc
     7321 gaccatctgc tgcgacatgc tgaagcgcga aaactcaggg tacgcatgtc cgaagacgag
     7381 tcgtgagtga ggagttttcg attcgaggga aagctggttc agagtgaaag cgtcggcata
     7441 ctcgcgatcg tcgttgcaat aggtgctgtc gagctggagc gacaggtagt gcccgtccag
     7501 gcgggttgaa tacagatcgt accgaatatt ctgttcgtag taggtgcttt gagagcgact
     7561 cgacaggttt cgagcgccat caaccattgt cccatagagc gttgtgtcgg ttaccagctg
     7621 ggacgtatag agtttctcgt cttcgctttc gttgaggttg cgcagttgca gttcgcggtc
     7681 atcacccagc gcgaggctgg tcgacacttc ggggggacgc atctgcgagg ctggtcgggt
     7741 ctgcacgatg tggctgccga gatccatggt tggcggtgga acgaatggcg gctcggaaag
     7801 cgcagtcgga tccttgccgg aaaactccgg gagatagcgc gggttga
//
LOCUS       DAORHD010000040         7775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9004_length_7775_cov_3.720207, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000040 DAORHD010000000
VERSION     DAORHD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9004_length_7775_cov_3.720207"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKO06_01640"
     CDS             complement(<1..1060)
                     /locus_tag="PKO06_01640"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HNV68371.1"
                     /translation="MAASSLPEPPLRAATLFPASYPGLFLLFAAPAFWFAGSRLVREW
                     SNNPSGELLPIVCITALALWWQQTSDHSLSLTTHPHRTWSENRFLPPVLLIASCCLLT
                     IDAMIEEESILALGIWSGVCGLVFLLARSQTQTRVRFPLLLLLFCLPLPGYVAFHYVH
                     YPLQKLSTLWTTGMLNIFGHQADSSGTIITIGSRTLQVVEECSGVRFLTALTFTALLA
                     GHLRLRSHAHLRMVLFLSAIPLAILTNVLRLTAAGHILIWRGPEAANAFIHGSAVGVF
                     YLAAISLLIVLTRLFIGLTQLPIVLNDSINQDILEVAAAGSLNVSGDESEGRDANHAI
                     ERRSESHAPPYAMLGRGVV"
     gene            complement(1070..1801)
                     /locus_tag="PKO06_01645"
     CDS             complement(1070..1801)
                     /locus_tag="PKO06_01645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HNV68372.1"
                     /translation="MNTSRTNHKTFLLIAWLLLLGSSVSAYQWNTVGGPLGTNWGPQG
                     TTVTYSFMPAGLNTDGGHTTSAIPGTWNAIITAAFNEWSSHCGLTFVQHPDSGLDFDA
                     TGATADIRIGRHHFDAALGTLAHGYYPPPNGDSAAGDVHFDEDEDWYVGLGVPGINQI
                     DLFSVALHEIGHAIGLGHSADPTSVMDPTYAPGSTLRDLQPDDIAGIQSHYGESGATP
                     EPATLLLFALCLGALFFMGPGGTRR"
     gene            complement(1776..2993)
                     /locus_tag="PKO06_01650"
     CDS             complement(1776..2993)
                     /locus_tag="PKO06_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68373.1"
                     /translation="MIFRILKVSTGDGPSTGFFFSRGLFTVLIVMVGAFGFMLAALVQ
                     HLWPAPETPTPVTRFPDTYTARFPATTAVPPPPAREVPASISASLSSLLHENAFESVL
                     TEVGSLSASYPGSRIPLLYRIQALVQLGRHSEAEQNIREALRAYPSDPETLIIGASYY
                     HAIRNPLAAEKMLLRALKADPNRVGAMRGLVTTYLQAGLWRLAKEIGINSLHKFPADP
                     VLTLGTADAARLLGEHAEAANRYQEYLRLQPHSRPDVHFSLATSLRESGAESNTVCEQ
                     YQKALSLQASHVPTLNDYALFLATKGRTQEAISLVPALLKHSATATIALDTAGLVHLK
                     ARQVAQAEMFFRQALAGEPHNPIIAAHLAALCHQQADQSKADEWLSRAKAECKGDQVE
                     WKRALGEYESHKP"
     gene            3355..4479
                     /locus_tag="PKO06_01655"
     CDS             3355..4479
                     /locus_tag="PKO06_01655"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV68374.1"
                     /translation="MAEKPKVFTVGLRAKGPSDSHLAKIEKLARHIGMGEFAWKNKIV
                     AVKTHFGERGNSSFPRPHYVRPLVDLIKAGGGKPFLAETTTLYTGSRSNAPDHIETAL
                     RHGFGAEVTGAPVIICDGLTGRDAHKVPINGRLLKEVSVAAGIVEADALVVLSHFKGH
                     EVSGFGGAIKNLGMGCCSRSAKLIQHTSIRPSVLTDRCVRCRTCLSWCSVGAIRQEQP
                     NGPVTIDPNVCQGCGECLVSCRVGAIRINWSEDAGKVTERMVEHALGVMENKKGRAFF
                     VNLLTDIVPLCDCYGFSDAPIVPDIGFAASTDPVALDACCVDLVNAQIGLQNSALKGG
                     YQPSEPKFAHVHEELDISIQLNYGTELGLGNAEYERVTLD"
     gene            4457..7162
                     /gene="mutS"
                     /locus_tag="PKO06_01660"
     CDS             4457..7162
                     /gene="mutS"
                     /locus_tag="PKO06_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNV68375.1"
                     /translation="MSVSPSTEPVPEATENVVTAPVSESGGTSAPESSAEKGMTPLMQ
                     QYVDIKAKYPEAILLFRCGDFYETFFEDAVVAAKELSIVLTARGRAKGEPVPLAGVPY
                     HSAMNYISRLVNKGFTVAICEQLEDPKLAKGLVKRDVVRVMTPGTLSDPQLLDETSNN
                     FLVAFGRRQGKFTLAAVEISTGEFLVTSGDQAEEHLLPEEFFRLRPREILALETSEDA
                     AVTAGPVAVPPWQALKAAVHQRKISEKDAIEALATLFPSEHRARFLTLPPLESRVLGI
                     LSEYLLDTQKCSLAHLRFPQPYRLGDGMVLDEATLRNLELLPDGRGQALGGTLFEAMN
                     QTRTSMGARLLKRWLTKPLIQLAPIRERQERVAAMVADGMLLGDLRGLLDRLPDFERL
                     MSKMALGGRSPRDLRALGVALDRLPIIRQRLAGTPLEPLAAALQVPPELASEILRSLK
                     EELPPTMADGGFIADGIDAGLDELRGLQRDGDRWLTAFEERERQESGIKTLRVRKNSV
                     FGYFIEISKGLADKAPAHYVRKQTLTTGERYITAELKEYETKVFSANEKSIALEKQLF
                     ETLFSRMQPHIPAIQTAGGALSEIDCLASLAQMARDRGYVRPELTEDLTLHIRGGRHP
                     VVEAFLGSGEFVPNETELDASRLQAIITGPNMAGKSTYLRQNALIVLLAQIGSFVPAA
                     EARIGVVDRIFTRVGAADNLIRGQSTFMVEMMETSSILRHATARSFLIIDEIGRGTST
                     FDGLSLAWAILEYITERIRARTLFATHYHELTDLPTVRPTLFNLNVAVAHDEKSGNMV
                     FLHQIQKGAANRSYGIEVARLAGLPAPLLDRAREILFELEKTEEEEVGRMTRTLKTSE
                     KAPLQLSLFAPEHELIEAVRNINLDHTTPLEALNLLARLKAMTDA"
     gene            7155..>7775
                     /locus_tag="PKO06_01665"
     CDS             7155..>7775
                     /locus_tag="PKO06_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68376.1"
                     /translation="MPDAYWDPIFARLTDDLDCVRQMAENLPDRALLEPIRGNPPLWK
                     QVQVFAKGDRFLNRARALEVILQSASHEPTLRRVLLWSWVQANDQTLKFVTLPLDRDS
                     EKRLLQGEFGNLAKLAILARIDPRESAKPLYEKVLAKLRADVPDTAQNLARATSSNSP
                     VVDAARAGELRECPSGNEVTSSAEAKNAVSENRELAALKKEMEQTRA"
BASE COUNT         1651 a         2167 c         2405 g         1552 t
ORIGIN      
        1 caacaacacc gcgaccgagc atggcatacg gaggtgcgtg cgactctgat cggcgctcga
       61 ttgcgtggtt tgcatcccgg ccttccgatt catcgcccga cacattcaac gacccggcgg
      121 ccgcgacttc gagaatgtcc tggttgatcg aatcgttgag aacgatcgga agctgagtaa
      181 gaccgatgaa caaccgcgtc aggacgataa gcaggctgat ggcagcgagg taaaacactc
      241 cgacagcaga gccgtgaatg aaggcgttcg ccgcttcagg accgcgccag atcagaatgt
      301 gtcccgctgc tgtcagccgc agcacgttcg tcagaatggc cagaggaatg gcgctcagaa
      361 acaaaaccat gcgtaggtgc gcatgggacc gcaggcgtag atgccctgcc aacaatgccg
      421 taaaggtcag cgcagtcaga aacctgactc cgctgcattc ttccaccacc tgaagggtac
      481 ggctcccgat tgtgatgatg gttcctgagc tgtcggcctg atgtccgaag atgttcagca
      541 ttccggtggt ccagagtgta ctgagcttct gtagcgggta gtggacgtag tgaaaagcca
      601 cgtagcccgg taaaggcagg cagaacaaca gaagcagcaa tggaaagcgg acgcgggtct
      661 gggtttggga gcgggccagg agaaagacaa gaccgcaaac gccactccag atgccgagag
      721 ccagaattga ctcctcctcg atcatggcat cgatcgtcag cagacaacat gatgcgatga
      781 gcaaaacagg tggcagaaat cgattttccg accatgttcg atgagggtgg gtggtgagtg
      841 acaaggagtg gtcgctcgtt tgctgccacc agagcgccag ggccgtgatg cagacgatgg
      901 gcaggagctc tccggatggg ttgttcgacc attcccggac tagtctcgag cccgcgaacc
      961 agaaagctgg tgccgcgaac agcaggaaca ggccaggata gctggctggg aacaaggttg
     1021 cggcccggag gggcggttca ggtagtgagg aggcagccac gaatttggtt cagcggcggg
     1081 tgccacccgg ccccatgaag aacaaagctc ccagacagag ggcaaacaga aggagtgtcg
     1141 ctggttccgg ggtggcacca ctttctccgt aatgggactg aatgccggca atgtcgtcag
     1201 gctgcagatc gcgcagggta gaccctggtg cataggtggg atccatgact gaagtcggat
     1261 ccgccgaatg accgaggccg atggcatggc cgatttcatg cagagccacc gagaaaaggt
     1321 caatctggtt gataccggga acaccgagcc ccacatacca atcttcatcc tcgtcaaaat
     1381 gcacgtcgcc cgccgcagag tcgccgttcg gaggtggata gtacccgtga gcgagagtgc
     1441 cgagtgctgc gtcaaagtga tgtcggccaa tgcggatgtc ggcagttgcg ccagtcgcgt
     1501 caaagtcaag accgctgtca gggtgttgca caaacgtgag cccgcaatgt gacgaccatt
     1561 cgttgaaagc cgccgtgata atggcattcc aggttccggg aatggcgctc gttgtatgcc
     1621 cgccgtccgt gtttagaccg gcaggcatga acgagtaggt gaccgtggtc ccctggggcc
     1681 cccaattcgt gcccagtgga cctccgacgg tgttccactg gtaagctgaa acggaggacc
     1741 cgagcaggag cagccacgca atcaacagga aagttttatg gtttgtgcga ctcgtattca
     1801 cccaatgccc ttttccattc tacctgatcg cccttgcatt ccgctttggc acgactcagc
     1861 cattcatcag cttttgattg atcagcctgt tgatggcaca aagctgcgag gtgggcagca
     1921 atgatcgggt tgtgcggttc gccagcgagg gcctgcctga aaaacatctc agcctgtgca
     1981 acctgtcggg ccttcagatg aacgagaccg gcggtgtcca gcgcgatcgt ggccgttgcg
     2041 gagtgcttca gaagcgcggg gacaagagag atggcttcct gggtccggcc tttggtggca
     2101 aggaaaagcg catagtcgtt cagcgttgga acgtgtgaag cctgaaggct gagtgccttc
     2161 tgatactgct cgcagacggt gttgctttct gcacctgact cccgaaggct ggttgccagc
     2221 gaaaaatgaa catccggtcg agagtggggc tgtagtcgca gatattcttg gtagcggttc
     2281 gcggcttccg catgttctcc cagcagtcgt gcggcatccg cggtaccgag tgtcagaacc
     2341 gggtcagccg gaaacttgtg caggctgttg atgccgatct cttttgccag acgccagaga
     2401 ccagcctgaa ggtaggtggt gaccagcccg cgcatggctc cgacgcggtt ggggtctgcc
     2461 tttagtgctc tcaagagcat cttctcggcc gcgagtggat tgcggatggc atggtaatag
     2521 gaagctccga tgattaaagt ctcggggtcg ctcgggtaag cacgtaaagc ttcgcgaatg
     2581 ttttgttccg cctcgctgtg cctgccgagc tgcaccaagg cttgaattcg atagagcaac
     2641 ggaattctgg aaccggggta ggatgcgctg agagagccta cttccgtcag gacggattcg
     2701 aatgcgttct cgtggagcag cgaactgaga gaagcggaaa tcgatgccgg aacttcgcgc
     2761 gcaggcggcg gtggcacggc tgtggtcgcg ggaaaacgag ccgtgtaggt gtcaggaaac
     2821 ctggtgaccg gcgttggggt ttcgggtgcc ggccagaggt gttgaaccag cgcggccaac
     2881 atgaacccga atgcccctac catcactatc agaacggtaa acaggccgcg cgaaaagaaa
     2941 aaaccggtag aggggccgtc tccagtactg accttgagaa tgcggaaaat catacctcaa
     3001 acctgaacca ggaaatgctt ctcgtcaatc aataaagcag atagatcacg aaaccggttc
     3061 acgcaattcc ttcgccgttc tcgtgaaatc gcgattgtaa aagctttttc ggcatttcag
     3121 aatagataca tgtattatta taatactgat aatatcatta caatttaatt gatattatca
     3181 gtattgcgat tttttctgga tcttggctag agtattcctt cagaacatcg gacagctttc
     3241 cctattcgtg aagtagttcg ggaaacagat tcagtgaaaa gtcgacgttt tggagcgagt
     3301 cgcggcgatg aggaccgtgg tataatcgct tcgattccag caccggaggc agatatggcc
     3361 gagaaaccca aagtgttcac cgtcggattg cgggcgaaag gcccgtctga cagccatctc
     3421 gcgaagatcg agaagctggc gcgtcacatc ggcatgggcg aattcgcctg gaagaacaag
     3481 atcgtcgccg tgaagaccca cttcggtgag cgcggcaact cctcgtttcc gcggccgcac
     3541 tacgtcagac ccctggtcga tctcatcaag gccggcggtg gtaagccgtt cctggctgag
     3601 accaccacgc tctataccgg cagtcgcagt aacgcccccg atcacatcga aaccgctctg
     3661 cgacacgggt tcggcgccga agtgaccgga gccccggtca tcatctgcga cgggctcacc
     3721 ggccgcgatg cccacaaggt gcccatcaac ggccgcctgc tcaaggaagt cagcgtggcc
     3781 gcgggcatcg tcgaagccga cgccctggtc gtgttgtcgc acttcaaagg ccacgaagtc
     3841 agtgggttcg gtggcgcgat caagaacctt ggcatgggtt gctgttcccg ctcggcgaag
     3901 ctgattcagc acacctcgat tcgtccttcc gtgttgaccg accgttgtgt ccgctgtcgt
     3961 acctgcctga gctggtgttc cgtcggggcg attcgccagg agcagccgaa tgggccggtg
     4021 accattgatc cgaacgtctg ccagggctgt ggcgaatgcc tggtttcgtg ccgggtgggc
     4081 gcgattcgca tcaactggag cgaagacgcc ggaaaggtca cggaacgcat ggtcgagcat
     4141 gcactcgggg tcatggaaaa caagaaaggt cgcgcctttt tcgtgaacct gctgaccgat
     4201 atcgtgccgc tgtgcgactg ctacggcttt tctgatgccc cgatcgtgcc ggatatcgga
     4261 tttgccgcat cgaccgaccc ggttgcgctc gatgcctgtt gcgtcgatct ggtgaatgcg
     4321 cagatcggtc tgcagaactc ggccctcaaa ggtggctacc agccttccga accgaagttc
     4381 gcgcatgtgc atgaagaact cgatatctcg attcagctga actacggaac cgaactcggc
     4441 cttggaaacg cggagtatga gcgtgtcacc ctcgactgag cctgtgcccg aagcgaccga
     4501 aaacgttgtg accgccccgg tcagcgagtc cggggggacc agcgcgcccg agtcgtcggc
     4561 agaaaagggc atgacgccat tgatgcagca gtatgtcgac atcaaggcga aataccccga
     4621 agcgattctg ctgtttcgct gcggtgattt ctacgaaacc ttctttgaag atgccgtcgt
     4681 agcggcgaaa gagctgtcga tcgttctgac ggcccgcggc agagccaagg gcgaaccggt
     4741 gccgctggcc ggcgtgccgt atcacagcgc gatgaactat atctcgcggc tggtgaataa
     4801 agggtttacc gtcgcgatct gcgagcagct cgaagacccg aaactggcga aagggctggt
     4861 gaagcgagat gtcgtgcggg tcatgacgcc gggcacctta tctgacccgc agctgctcga
     4921 tgaaaccagc aacaactttc tggtcgcgtt cggccgccgt caagggaagt ttacgcttgc
     4981 cgccgtcgag atttcgaccg gggagttcct ggtgacttcc ggcgatcagg ctgaagaaca
     5041 cctgttgccc gaggagtttt tccgactgcg gccacgcgaa attctggcac tcgaaacgtc
     5101 tgaagacgca gctgtgacgg cggggcctgt ggctgtgccg ccgtggcagg cgctcaaggc
     5161 tgcggttcat cagcgcaaga tttctgaaaa agatgcaatc gaagctctgg cgacgctctt
     5221 tccgtctgaa catcgggcac gttttctgac gctgcccccg ctggaatcgc gtgtgctggg
     5281 cattctctcc gagtatctgc tcgatacgca gaagtgctcg ctcgcgcatc tgcgctttcc
     5341 gcagccgtat cggctgggcg atggcatggt gctcgacgag gcgacgttgc gcaacctcga
     5401 actgctgccc gacggccgcg gacaagctct gggcggcacg cttttcgaag cgatgaatca
     5461 aacccgcacc tcgatggggg cgcggctgct caagcgctgg ctgaccaagc cgctgatcca
     5521 gctggcgccg attcgtgagc ggcaggaacg ggttgcggcc atggttgccg acggcatgct
     5581 gctcggcgac ctgcgcgggt tgctcgaccg cctgcccgat tttgaacgtc tgatgtcgaa
     5641 aatggccctg ggtggccgtt cgccgcgcga tttgcgagct ctgggagttg cgctcgaccg
     5701 cctgccgatc attcgacagc ggctggccgg aactccgctt gagcctctgg cagctgctct
     5761 gcaggtgccg cccgaactgg cgagtgaaat tctgcgttcg ctcaaggaag aactgccgcc
     5821 gaccatggcg gatggcgggt tcatcgccga tggaatcgat gcgggtctgg acgagctgcg
     5881 tggcttacag cgggatgggg accgctggtt gacggctttc gaagagcgcg aacggcagga
     5941 aagcgggatc aagaccctgc gggtccgcaa aaacagcgtg ttcgggtatt tcatcgagat
     6001 ctcgaaaggg ctggcggaca aagctccggc gcactacgta cgcaaacaaa ccctgacgac
     6061 gggtgaacgc tacatcacgg ccgaactcaa ggaatacgag accaaggttt tttcggccaa
     6121 cgagaagtcg atcgccctcg aaaagcagtt gtttgaaacc ctgttttccc gcatgcagcc
     6181 gcacattccg gccattcaga ccgcgggcgg cgctctcagc gaaatcgact gtctggcctc
     6241 gctggcccag atggcccgtg atcgcggcta tgtgcgcccc gaactcaccg aagacctgac
     6301 gctgcacatt cgtggtggtc ggcatccggt ggttgaggcg tttctgggct ctggcgagtt
     6361 tgtcccgaac gagacagagc ttgatgcgtc gcgtttgcag gctattatca ccggtccgaa
     6421 catggccggt aagtcaacct atctgcgaca gaatgcgctg atcgtactgc tggcgcagat
     6481 tggctcgttt gtcccagctg ctgaggccag gatcggcgtc gtcgaccgta ttttcacccg
     6541 ggtcggtgct gctgacaacc tgattcgcgg tcagtcgacc ttcatggtcg agatgatgga
     6601 aacctcatcg attctgcgtc atgccactgc ccgcagcttt ctgatcatcg acgagatcgg
     6661 gcgcggcacc tcgactttcg acgggctgtc gctggcctgg gcgattctcg aatacatcac
     6721 cgagcggatt cgagcgcgaa cgctgttcgc cacgcattat catgaactga ccgatctgcc
     6781 caccgtgcga ccaaccctgt tcaacctcaa cgtcgccgtg gcgcacgatg agaagtcggg
     6841 caatatggtt ttcctgcatc agatccagaa aggggcggcc aatcgctcgt atggcatcga
     6901 ggtcgcgcgc ctggccgggc tgccggcacc tctactggac cgcgcccgcg agatcctctt
     6961 tgagctcgaa aaaaccgaag aggaagaggt cggtcgcatg acccggacac tgaaaaccag
     7021 tgagaaagcg cccctgcagc tttcactgtt tgctcccgaa cacgagctga tcgaagcggt
     7081 tcggaacatc aacctcgatc atacgacgcc gctcgaggcg ttgaacctgc tggcgagact
     7141 gaaggcgatg accgatgcct gacgcttact gggatccgat ctttgcccga ctgaccgacg
     7201 atctcgactg cgtgcggcag atggccgaaa atcttcctga tcgtgcgctg ctcgaaccga
     7261 ttcgcgggaa cccgccgctc tggaagcagg tgcaggtctt tgccaaggga gatcggtttc
     7321 tcaaccgggc ccgggccctc gaggtgattc tgcagtcggc gtcacatgag ccgactctgc
     7381 ggcgggtgct gctctggagt tgggtacagg ccaacgatca gacattgaaa tttgtgaccc
     7441 tgccgctcga ccgtgacagc gagaaacgtc ttctgcaggg cgagttcgga aacctggcca
     7501 aactggcgat tctggcgcgg atcgatccgc gtgagtcggc gaagccgctc tacgagaaag
     7561 tgctcgcaaa actgcgggct gatgtccccg acactgccca gaaccttgcc cgcgcgacct
     7621 cttcgaacag cccggtggtc gatgcggcac gtgcaggtga gttgcgcgaa tgtccgagcg
     7681 ggaatgaggt gacttcgtct gctgaagcga agaacgctgt ctcggaaaac cgggagctgg
     7741 cagccctgaa aaaagaaatg gagcagacgc gggct
//
LOCUS       DAORHD010000041         7770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9018_length_7770_cov_4.004407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000041 DAORHD010000000
VERSION     DAORHD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7770
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9018_length_7770_cov_4.004407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="PKO06_01670"
     CDS             <1..612
                     /locus_tag="PKO06_01670"
                     /inference="COORDINATES: protein motif:HMM:NF022273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HNV68377.1"
                     /translation="SLNEETSERKIVSTSETPSQTAAIVKEQLVQKVEPVTVAPVKEV
                     VAPVVTEPVAKVSEKPVVAVAVESKPAVTTEKGVPAAAYQILYLFQKEGRLLDFLMED
                     ISSVDDDQLGGAIRPIHEGCRKILQERLKIEPVLQGPEGETVKVPEPHDPEEVKLTGN
                     VPAKGPFSGTLVHRGWRLTECKLPELVAGWSGDVVAPAEVEIS"
     gene            726..2510
                     /locus_tag="PKO06_01675"
     CDS             726..2510
                     /locus_tag="PKO06_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV68378.1"
                     /translation="MSQPKNIVGIDLGTTKVVLTYAKMPASEDDKPSIDLFPLLQEFA
                     KESMEERDILPSFLFDRLQEKPVLPWKREQSFIVGEYARERGSEVPARLVSSAKSWLC
                     NPRVNRRENILPWNAPADARKISPLDAQTELLHHVKACWNNKFKQKLEDQMVVITIPA
                     SFDAVARDLTLEAARQAGLENVTLLEEPQAAFYSWISQSEAPWRDQVRKGDVVLVVDV
                     GGGTTDFSLIEIGEEGGDLNLSRVAVGEHILLGGDNMDLTLAFHVKQKLEAAKKKIDT
                     WQTIALSHACRKAKERMLADTSIKSEPIVVSGRGSSVIGGSMKTTLEREEIQNLLVEG
                     FFPTCALSDEPQETSESGFREVNLAYASDPAITRHLARFLNSQGGTKEYKYPNAVLFN
                     GGVFKSDKCRERLTEVINSWLQAAGKPEVRVLQGADLVRAVSFGASYFGLARQGKGIR
                     IRGGVSQAYYLGIEMSMPAVPGIKPPVKALCVAAIGMEEGTKTGVPDRTFGLVVGRPV
                     QFKFFRSPTRREDQVGHLFDDILAEIEETSSLALNLPAAEGMTPGSTVDVTLEVAITE
                     IGTLEIWCLQKGAANKWKLEFNVRDAIK"
     gene            2687..3358
                     /gene="aat"
                     /locus_tag="PKO06_01680"
     CDS             2687..3358
                     /gene="aat"
                     /locus_tag="PKO06_01680"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNV68379.1"
                     /translation="MTPPRHRIPFLGLKLWFPDPRQADRDGLLAAGGDLSPERLLLAY
                     RSGIFPWTYDPITWWSPDPRAVFEIADFHVPRRLERTLRQGSFTITFDQAFRQVMSAC
                     AEPRPSGEETWISPEFIDAYTELHRQGHAHSVEAWLEGRLVGGVYGVAVGGLFAGESM
                     FHRVSNASRAALSILMRHLQARGFHLFDSQVITPATERLGVRQITRDEYLRRLAAVVD
                     LPVSF"
     gene            3426..3740
                     /gene="clpS"
                     /locus_tag="PKO06_01685"
     CDS             3426..3740
                     /gene="clpS"
                     /locus_tag="PKO06_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011646051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNV68380.1"
                     /translation="MPVPHTRHEEVLEEKTASKSKKPSLYRVFLLNDDYTTMEFVVYV
                     LETIFRKPSAEATRIMLHVHKKGKGLAGVYPRDIAETKITSVEELARTQGFPLRCEME
                     KE"
     gene            3737..6052
                     /gene="clpA"
                     /locus_tag="PKO06_01690"
     CDS             3737..6052
                     /gene="clpA"
                     /locus_tag="PKO06_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010363140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNV68381.1"
                     /translation="MINKELQLAIEAAIRDAARGHHEYVTVEHLLYAVVHDDYGRDVL
                     HSCGGDLGRIKRKVEEYFDKQVPRFPQNSQDQPQITVGFQRVMQRAFLHVQSAGKSEA
                     NAGDVLAAIFLEDDCQARHILESEDITRLDVLNFVSHGVVKEGFPLGEDGKNALEGEQ
                     IGGDEDDDEGEQTDFPGGEARPKPVRDPLKQFATNLLERAAAGEIDPLVGRIRELRRT
                     IQILARRKRNNVVFVGEPGVGKTALVEGLALKIHQREVPEILLDTPIYALDMGGMLAG
                     TKYRGEFEARLKATIKALSEKPKVILFIDEIHTIVGAGATSGGVMDASNLLKPLLNSG
                     KIRCLGATTYEEYRQVFEKDRALARRFQKMELQEPTVRETVKILKGLRRYYEEFHQVR
                     FPCATLRAAAELSAKYVNERFLPDKAIDMIDEAGARLKLSPQYPKKKTVTVRDVERVV
                     SLMARIPAKTVSSNEVEKLGQLDQSLKQVVFGQDEAINQLVSSIKRTWAGLGSPDRPL
                     GSFLFIGPTGVGKTEVCKQMAAVMGVKFLRFDMSEYMEKHAVSRLIGAPPGYVGFDQG
                     GLLTDGVRKNPYCVVLMDEIEKAHQDVFNILLQVMDYATLTDNNGKKADFRHVILVMT
                     SNAGAQEMEKQSIGFGEQKLESSDKAREAVNRLFSPEFRNRLDGIISFRRLARETMVQ
                     IVGKNLREVERQLERKEIVLEVSEAARHWLAEHGYDPRQGARPLARLVQKELKDPLSE
                     QILFGALKQGGKALVDLRDGKIAFTFESKAA"
     gene            6054..>7770
                     /locus_tag="PKO06_01695"
     CDS             6054..>7770
                     /locus_tag="PKO06_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV68382.1"
                     /translation="MNQAREAGPRYLIGIDLGTTQSAISYYDAENPESGIQSLLVPQR
                     VSAREISPFPTLPSVAYLAEETLELAGFEVEVPVGDSGHRPVVGHWARELGSQVPARL
                     VASAKSWLCHPRIDREAAILPWQSETVRDRISPVTAARLYLSHLKQIWNRSLGARGEE
                     HRFEAQKIVVTVPASFDPVARNLTLKAVEEAGFPPVTLLEEPQAAFYDFLSRHQETVA
                     GALDAVKTILVIDIGGGTTDFSLIGVEWSKESRAGRAQPKLSRLAVGPHLLIGGDNFD
                     LTLAHTVEGRLRERGRKLVTRQWQALLYQAREVKERLLSLKPPEKVTFQIPGAGSRVV
                     AQTLKEELAAEPLREQLLSGFLPTVAATDRPEEDTGLGISDAGLPFTRDPAITRHLAA
                     FLADQRVFPDAVLFNGGTTLSPALRDRLLQQLTAWSQTGTAPKVLENPYPTMAVAHGA
                     VFFAQVATGTGLRIQSGSPNSIYLGIGTDPASTPARHIPESLICLLPKGAEPETDYQI
                     ASKVFGIDRRRPVAFYLYYSGNPPRGEGAGDTITYQARRHRPLPPPRLQTAGAKGGAS
                     AERVEE"
BASE COUNT         1625 a         2182 c         2421 g         1542 t
ORIGIN      
        1 agtctgaatg aagaaacttc ggagaggaaa attgtttcaa cctcagaaac tccgtctcaa
       61 acagctgcga ttgtcaaaga acaacttgta caaaaagttg aaccggtcac agtcgcgccg
      121 gtgaaagagg tggttgctcc ggtcgtcacc gagcctgtcg ccaaggtgag tgagaaaccg
      181 gtggttgcgg tggccgtcga gtcgaaaccc gccgtcacga cggaaaaagg agtgccggcc
      241 gcggcgtatc agatcctgta tctgtttcaa aaagaaggac gactgctcga tttcctgatg
      301 gaagacattt cgagcgtcga tgatgatcaa ttgggcggag ccatacgccc cattcacgag
      361 ggctgccgca agattctgca ggaacggctg aagatcgagc ccgtgttgca gggtccggaa
      421 ggcgagacgg tcaaggtgcc ggaaccgcac gacccggagg aagtgaagct gaccggaaat
      481 gtaccagcca agggcccctt ctccggaact ctggtgcatc gcggctggcg tttgaccgag
      541 tgcaaactgc ctgagctggt cgccggttgg tccggtgacg tggtcgctcc cgcggaggtt
      601 gagatctcgt gagctgaacg gaaacgttct cgagaaaccc ggtcgcggca tggtcgcagg
      661 ccgggtttct tttttgactc cgatttgagt ataataagcg ctcaaacaag cacaaggaga
      721 cgcgcatgag ccagccgaag aacatcgtcg gcatcgacct gggaacgacg aaggtcgttc
      781 tgacctatgc caagatgcct gcttccgaag acgacaagcc gtccatcgat ctgtttcccc
      841 tgctgcagga gttcgccaag gagagcatgg aggaacgcga catcctgccc tcgttcctgt
      901 tcgaccggtt gcaggaaaag ccggttctgc cctggaagcg cgagcaatca ttcatcgtcg
      961 gcgaatacgc ccgtgaacgt ggctcggaag tgccggcccg cctggtttct tcggccaagt
     1021 cgtggctgtg taatccgcgc gtgaaccgcc gggaaaacat tcttccctgg aacgcccccg
     1081 ccgacgcccg caagatctcg ccgctcgatg cgcagaccga gctgttgcac cacgtcaaag
     1141 cctgctggaa caacaagttc aagcagaagc tcgaagatca gatggtcgtc atcacgattc
     1201 cggcgtcgtt tgatgcggtg gcccgtgatc tgacactcga agcggccaga caggccgggc
     1261 tcgagaatgt gacgctgctc gaagagccgc aggccgcctt ctattcctgg atctcacagt
     1321 ccgaagcccc gtggcgcgat caggtgagga agggagacgt cgtgctggtt gtcgacgtcg
     1381 gcggtggaac caccgacttc agtctcatcg agattggtga agaaggggga gacctgaacc
     1441 tcagtcgcgt ggccgtcggc gaacacattc tgctgggcgg cgacaacatg gatctgacgc
     1501 tggccttcca cgtcaagcag aagctcgagg ccgccaagaa gaagatcgac acctggcaga
     1561 ccatcgctct cagccatgcc tgccggaaag ccaaggagcg catgctggcc gatacgagca
     1621 tcaagtccga gccgatcgtc gtctcgggcc gcggctcgag cgtgattggc ggcagcatga
     1681 agaccactct cgaacgtgaa gagattcaga atctgctcgt cgaagggttc ttccccacct
     1741 gtgccttgtc cgatgaacca caggagacgt ccgaaagcgg attccgtgaa gtcaatctgg
     1801 cctatgcctc tgacccggcc atcacccgcc atctggcgcg ctttctgaac tcgcagggtg
     1861 gtacaaaaga gtacaagtat ccgaacgccg ttctcttcaa tgggggcgtc tttaaatctg
     1921 ataaatgccg cgaacgcctg accgaggtga tcaactcctg gctgcaggcg gcgggaaaac
     1981 ccgaggtgcg cgtgctgcag ggcgcggatc tcgtgcgggc tgtctctttc ggagccagct
     2041 acttcggcct ggcccgccag ggaaaaggga ttcgcattcg cggcggcgtg tcacaggcct
     2101 actatctggg catcgaaatg tcgatgccgg cggttccggg catcaaacct ccggtcaagg
     2161 ccctctgcgt tgccgccatc ggcatggagg aaggaacgaa gaccggagtc cccgatcgca
     2221 cctttggtct ggtcgtcggg cgaccggtac agttcaaatt tttccgttcg ccgacccgtc
     2281 gtgaagatca ggtcggacac ctgtttgacg acattctcgc cgagatcgaa gaaaccagca
     2341 gtctggcgct caatctgccg gccgccgagg gaatgacgcc gggaagcacg gtcgatgtca
     2401 cgctcgaagt ggcgattact gagatcggca ccctggaaat ctggtgtctg cagaaggggg
     2461 ccgcgaacaa atggaaactg gaattcaacg tgcgggacgc cattaagtag cgattcatcg
     2521 ctgattcgac aacccaaccg acctttggaa agccgctgcc ttcgggcagc ggctgccgtt
     2581 atcagagctt tttcttcgtg tcatgtgaca gacgacgggt ttctgtttac aataggacga
     2641 gaaaatctcc tccaggtgag cggaactttc ccgacggctt gactccatga caccccctcg
     2701 acatcgcatt ccatttctcg gattgaagct ctggttcccg gatccgcgac aagctgaccg
     2761 ggacggactg ctggcggctg gtggtgatct cagtccggaa cgcctgctgc ttgcctaccg
     2821 cagcgggatc ttcccctgga cctacgatcc gattacctgg tggtctcccg atccgcgagc
     2881 cgtcttcgag attgccgact ttcatgtgcc acgacggctg gaacgcactt tgcggcaggg
     2941 gtcgttcacg atcaccttcg atcaggcgtt tcgccaggtc atgtccgcgt gtgccgaacc
     3001 gcgcccctcg ggcgaagaaa cctggatcag ccctgaattc atcgatgcct ataccgagtt
     3061 acatcggcag gggcatgcgc acagcgtcga agcctggctg gaaggacgtc tcgtgggtgg
     3121 cgtctatggg gtggcggtgg gcggcctgtt cgccggtgaa tcgatgttcc accgggtcag
     3181 caacgcttct cgtgcggctc tgagcatact gatgcggcat ctgcaggcgc ggggattcca
     3241 cctgttcgac agccaggtca tcaccccggc gaccgagcgt ctgggggtgc ggcagatcac
     3301 gcgtgacgag tatctccgcc gtctggcggc ggtggttgat ctgccggtca gcttctgatc
     3361 attgccctgc cccgattgtc gggcacgggc aggtgtggta gagtgctgat caggagatac
     3421 gctcaatgcc ggtaccgcat acccgtcacg aagaagttct cgaggaaaaa accgcgagta
     3481 aatcgaagaa accgtcgttg tatcgagtct ttctcctgaa cgacgactac acgaccatgg
     3541 aattcgtggt ctacgtgctc gagacgatct tccgcaaacc ttcggctgag gcgacacgca
     3601 tcatgttgca tgtgcacaag aaggggaaag gtctcgccgg cgtgtatcca cgtgatattg
     3661 ccgagaccaa aatcacctcg gtcgaagaac tggcgcggac tcagggtttt ccactgcgct
     3721 gtgaaatgga aaaagaatga tcaacaaaga actgcaactg gccattgagg ccgcgattcg
     3781 cgatgccgcc cgcgggcatc atgagtatgt caccgttgaa catctgctct acgccgtcgt
     3841 tcatgacgac tacggccgcg acgtcttgca cagttgcggt ggtgatctgg gccgtatcaa
     3901 acgcaaagtc gaggaatact tcgacaagca ggtgccgcgt tttccccaga actcacagga
     3961 tcaaccgcag atcacggtcg gttttcagcg ggtcatgcag cgcgcgtttt tgcatgttca
     4021 atcggcggga aagtcggaag ccaacgcggg agacgtgctg gccgccattt ttctggagga
     4081 tgactgccag gccagacata ttctggagag cgaagatatc acgcggctcg atgtgctgaa
     4141 cttcgtgtcg catggcgtgg tcaaagaggg ctttccgctc ggtgaagatg ggaaaaacgc
     4201 tctcgagggc gagcaaattg gcggtgacga agacgatgac gaaggcgagc aaaccgactt
     4261 tcccggtgga gaagcccgcc cgaagccggt gcgcgatccg ctgaagcagt ttgccaccaa
     4321 tctgcttgaa cgggccgccg ccggggagat tgatccattg gtcggtcgca ttcgtgagct
     4381 gcgccgaacc attcagattc tggcccgccg caagcggaac aacgtcgtct ttgtcggtga
     4441 gccgggtgtc ggtaaaacgg ctctggttga gggtttggcg ctcaagatcc atcagcgaga
     4501 ggtcccggag attctgctcg ataccccgat ctatgcgctc gacatgggcg gcatgcttgc
     4561 gggcaccaaa taccgcggcg agttcgaggc gcgcctgaag gcgaccatca aagcgctcag
     4621 cgagaagccc aaggtcattc tgttcatcga cgagatacac accatcgttg gtgccggcgc
     4681 gacgagcggc ggcgtgatgg atgcctcgaa tctgctcaag ccattgctga acagcggcaa
     4741 gattcgctgt cttggcgcga cgacctacga agagtatcgc caggtgttcg agaaagatcg
     4801 agccctggcc agacgtttcc agaaaatgga actgcaggag ccgacggtgc gcgaaacggt
     4861 caagattctc aagggtttgc gccggtacta tgaggagttt catcaggtgc gctttccctg
     4921 tgccacgttg cgagccgccg ccgaattgtc ggccaagtat gtcaacgaac ggttcctgcc
     4981 ggacaaagcg atcgatatga tcgatgaagc gggagcccgc ctcaagctgt cgccgcagta
     5041 cccgaaaaag aagaccgtga ccgtccgaga tgtcgagcga gtcgtgtcgc tgatggcgcg
     5101 cattccagcc aagaccgttt catcgaatga agtggaaaag cttggtcagc tcgatcagtc
     5161 gctgaagcag gtcgtctttg gtcaggacga agccatcaat caactggtca gttccatcaa
     5221 acgcacgtgg gcggggctgg gttctcctga tcgaccgctc ggctcgttcc ttttcatcgg
     5281 cccgaccggt gtcggcaaga ccgaggtctg caagcagatg gccgcggtga tgggcgtcaa
     5341 gtttctgcgt ttcgacatga gcgaatacat ggagaaacat gcggtgtcgc gcctgatcgg
     5401 cgcgcctccc ggatatgtcg gttttgatca gggtggcctg ttgaccgatg gtgtccgcaa
     5461 gaacccctac tgcgtcgtgc tcatggatga aatcgagaag gcgcatcagg atgtgttcaa
     5521 cattctgctg caggtgatgg actacgcgac cctgactgac aacaatggca agaaagcgga
     5581 ttttcgccac gtcatcctgg tcatgacctc taacgcgggg gctcaggaaa tggagaagca
     5641 gtcgatcggt ttcggtgagc agaagctgga gtcttccgac aaagctcgcg aagcggtgaa
     5701 tcgtctgttc agtcctgagt ttcgcaaccg tcttgatggc atcatcagct tccgccgact
     5761 ggcgcgcgag accatggtgc agatcgtcgg caagaacctg cgcgaggtcg agcgccagct
     5821 cgagcgcaag gaaatcgtgc tcgaggtgag tgaggccgcc cgccactggt tggcggaaca
     5881 tgggtatgat ccccgccagg gcgcgcgccc actggctcgt ctggttcaga aagaactcaa
     5941 agatccattg tccgagcaga ttctcttcgg agcgttgaaa cagggcggaa aagcgttggt
     6001 cgatctgcgc gatggcaaga tcgcgttcac gttcgagtcg aaagcggcct gacatgaacc
     6061 aggctcgtga ggctggcccg cgttatctga tcggcatcga tctgggcacg acccaatcgg
     6121 cgatcagtta ctacgatgcc gagaatccag agtctggcat tcaatcgttg ctcgtgccgc
     6181 agcgggtctc ggcgcgcgag atcagcccgt tcccgacgtt gccatcggtg gcctacctgg
     6241 ccgaagagac gctcgaactg gcgggctttg aggtggaagt accggttggt gattccggtc
     6301 atcggcctgt cgtcggtcat tgggcacgtg aactcggaag ccaggtgccg gcgcgtctgg
     6361 tggcgagcgc caagtcatgg ctctgtcacc cgcgtatcga tcgcgaagcc gcgattctgc
     6421 cctggcagag tgagacagtg cgcgatcgca tttcgcccgt gacggcggcg cgattgtacc
     6481 tgtcgcatct caaacagatc tggaaccgca gtcttggagc gcgtggagaa gaacatcgat
     6541 ttgaggctca gaaaattgtg gtcacggtgc cggcgtcctt tgacccggtg gcccggaatc
     6601 tgacgttgaa agcagttgaa gaggcgggtt tcccgccggt gaccctcctg gaagaaccgc
     6661 aggcggcttt ctacgatttt ctcagtcggc accaggagac ggttgccgga gcgctggacg
     6721 cggtgaagac gatactggtc atcgatatcg gtggcgggac gaccgatttc agtctgatcg
     6781 gggtggagtg gtcgaaagaa agtcgtgccg ggcgagctca gccgaagctg tcgcggttgg
     6841 cggtcgggcc gcatctgctg attggcggcg acaactttga tctgacactg gcgcacacgg
     6901 tcgaaggccg cttgcgtgaa cggggccgaa aactggtgac ccgacaatgg caagcgctct
     6961 tgtaccaggc gagagaggtg aaagagcgcc tgctgtcatt gaagccgcca gaaaaagtca
     7021 catttcagat cccaggtgcc ggaagtcggg ttgtggcgca gacactcaag gaagagctgg
     7081 cggctgaacc attgagggaa cagttgctgt ccggatttct gccgaccgtc gcggctactg
     7141 atcgccccga agaagatacg gggttgggta tttcagatgc cggcctgccg ttcacccgtg
     7201 atccggcgat tacccgtcac ctggctgcat ttctggctga tcagcgggtg tttcccgacg
     7261 cggtgctgtt taacggaggt accaccctgt caccggcgct gcgcgaccgc ctgctgcagc
     7321 aactgaccgc ctggagccag acgggaaccg cgccgaaggt gctcgagaat ccgtatccca
     7381 ccatggctgt cgctcatggg gccgtctttt tcgctcaggt ggcgaccggc accgggctgc
     7441 gcattcagag cggcagtccg aattcgatct acctcggcat tggtactgat ccggcgtcga
     7501 cgccagcccg tcatattccc gagtcactca tctgcctgct gccgaaaggg gctgagccgg
     7561 aaacggacta ccagatcgcc tcgaaggtgt tcggcatcga tcggcggcgt ccggtggctt
     7621 tctatttgta ctactccggc aatccgccgc gcggcgaagg cgcaggagat accatcacct
     7681 accaggcccg tcgtcaccga cctctgccgc ctccgcggct gcagaccgcc ggagcgaagg
     7741 gaggggcatc ggcagagcgt gtcgaggagg
//
LOCUS       DAORHD010000042         7736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9066_length_7736_cov_3.836610, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000042 DAORHD010000000
VERSION     DAORHD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7736
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9066_length_7736_cov_3.836610"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..2125
                     /gene="nifJ"
                     /locus_tag="PKO06_01700"
                     /pseudo
     CDS             32..2125
                     /gene="nifJ"
                     /locus_tag="PKO06_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718531.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
     gene            2238..3470
                     /locus_tag="PKO06_01705"
     CDS             2238..3470
                     /locus_tag="PKO06_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV68383.1"
                     /translation="MNSWTKTRLVTIVWFFLLACALGVSAAEGQLPAIFQQGTFEEVS
                     ARAEKEQRLLIVDFMATWCPPCKRMDQMTWPDMALMAWVASHALAIQIDVDKEAKLAE
                     NFGIEAMPTVLVLKRDKTLGRFTGFRDADDLLTWLEQVRTGKEPPPAPAAKNPREEGQ
                     RKMKEAREALAAGNFDAATEQFLWLWDHLLELSPSFVGVKYSFLLDDLKSLASKHAPA
                     RAKLAAMRDKLNAVVEAERVDPAQFKDWFSLCRIVGDDEKIMAWYEASAKSPSSLSRL
                     LPVQLDITDFLLEHKRLAEAGRLVTDPLTNVRAMLFMGKTGDLLEDASLARFIGGRWL
                     ALLAAGRRDEAVQIRKELFISLGQAPEVKINLMEAGADAGVRLPEFDVWIKEAQEKSK
                     DVASLSAALKNLPLLKVP"
     gene            complement(3479..5062)
                     /locus_tag="PKO06_01710"
     CDS             complement(3479..5062)
                     /locus_tag="PKO06_01710"
                     /inference="COORDINATES: protein motif:HMM:NF028512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68384.1"
                     /translation="MSFRESLNRSGLQQQLRIVFFCMVLPLFVVFGIFVNVREAMLIR
                     GAFEKETLRLARLAGYTLAPGVEFEDVKAVQEVINGLIQGGEVSYVIVTDRNQNVVAS
                     YQPENVGQKPFASFSGEHPAGAPATSTGVASTASVIDRGSVLHVQVPLQFNQRQLGEM
                     AIGFSLDRINSEITTNIAIMIGALLVGLLFIYIGARYFGNLIITPLLEIKKAAEHLSR
                     QEYDQVPAFVTAPQEIEYFYNTFVQMAEEIKRNHLALQNVNLTLEQKVAERTHELQIQ
                     TEKALQSDRLKSEFLANMSHELRTPLVSILAWPDIILEHYEMRDHVLTGAREIKKTGR
                     HLLSLINDLLDLESIDTGKMRVHLQSTDPIPLIIEAIEHTSGFAGTRQVEVQAQFEAD
                     VPHVEIDPIRFKQVMINLLSNAIKFSPEKNRVIVRAGVVGDLVEFSVQDFGRGIAPEK
                     QPIIFERFRQVDGTIRRKFGGSGIGLYLAKNFIDRMGGDIRVVSDVGKGSTFVVRLHQ
                     RTNSSIPDTNPDTDLQPEE"
     gene            complement(5059..5679)
                     /locus_tag="PKO06_01715"
     CDS             complement(5059..5679)
                     /locus_tag="PKO06_01715"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNV68385.1"
                     /translation="MSTLATSNLNLSSRRHRPVTVMARVLLLILFCCASMPCCAVSEE
                     EIPLDLQLQIFLKIMTYNRTLSFDKLDSFAVTIAYNKAAGKQSLEELSARIKDILGGK
                     TILGRPITLNIVAIGKAFKTSALNACHLLILHGELDEEKASLLAWANERKVLSAAAMH
                     DADVSDVVILLRLEDQKPVIHFNLGLARKLGFDFHAQFLKHCIITK"
     gene            complement(5657..7663)
                     /locus_tag="PKO06_01720"
     CDS             complement(5657..7663)
                     /locus_tag="PKO06_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV68386.1"
                     /translation="MTDKTASSHLRRWGAVFCIIAGICSCANHAAIAANASSTGFQDF
                     SEVSLDDLLNQVVVTAARHEQRLSDSPMTTHVITEEEIRLSPAITLADLLRMVPGFQS
                     KTWLSQFTNTSIRGMLGASVINERILWMVDGVPINDVRDGGIWTDITSIPLSNIKRIE
                     VLSGPGSPLYGSNAFLGVVHILTKSPDDYLKHGLTGQAQVSYGTFDTSINSLAVAGRN
                     GRAKWLWTADGGTTRGPGLVRDRNRPGEAKHTDRMWGFLRGKYEWGNHKVQVGARRVL
                     QDYDGADFAVYRLYTWGRDEKWLDWHFRQPRGQKIEDSFVFSWHAFNEHFYDFADVPD
                     LTYDIRSYRLYFNLQRDIQYRKHRLSIGTGLRSESYHGDDFYPDKRSILKENWNLFFQ
                     DLYKFDSRWSLSFGGRYDTHPNYDNIFSPHASLMHSFDSQRGKVRLTHGRAFKEPSNW
                     QSYIDQPSGKGTPDMTPEKLQANELAIEYTFPRELRLGATLFSFRHENIIWENFDPVV
                     ADPAYARYGILGKFHPQQPGDDAKIKGIEFEVQKRFSPKTRLFSNFVWLSSKDSRERD
                     LQYDAPRMISAGIWHTLNHRTSLHLVHHHVAETTDTNLAHVPGIGVRTVEAYSITGAS
                     LNLKLSERERIKLSAWNLGRGTYEEMIGAPVPGTTVHCEYTRHF"
BASE COUNT         1732 a         2069 c         2281 g         1654 t
ORIGIN      
        1 taccgttgga taaataattt tttgatttgg ccacaacttc agtttcatcg ataaatacga
       61 tatgctttcc gacctgaagg aaggtggctc tttcctgctc gagagcccgt tcaaggcttc
      121 cgagatctgg gacaaactgc ccgctgacat gcagcagcag atcatcgaga agaaggcgaa
      181 gttctacgtg atcgacgcgc tcagccacgc cgagaagctc ggcctgggca accgcatcaa
      241 catcatcatg cagtcagcat tcttcgccat cagcggcatt ctgccccgcg atcagttcat
      301 gaagagcatc aaggatgccg tcaagaagac ctacggtcgc aagggcgacg aagtcgtgcg
      361 catgaatcag gaagcggtgg atatcggcgc caacagttac gaagaagtca aatacagcaa
      421 agtcaccagc aagattccga tgcgcgtcgg cgttccgaaa gaggctcccg aattcgtgcg
      481 cgacgtgctc ggcaccatca tcgccggcaa gggggattcc atcccggtgt cgaagatgcc
      541 cgtcgatggt accttcccga cctcgaccac ccaatgggaa aaacgcagca tcgcaaccca
      601 gatcccggtg tgggaagcca acatgtgcat ccagtgcgga ctctgttctc tggtgtgtcc
      661 gcatgccgcc attcgcacca aggtctatga tccgaagtgg gccaaagaag cgccgaagac
      721 cttcaagagc tgcaaggcca ccgcgcctca gttcaaggat aaagatctgg cgttcaccgt
      781 ccaggtggcc tctgaagatt gcactggttg cggaacctgc gtctacatct gcccgaagaa
      841 agacaaagac agcggcaaga aagccatcaa catggctcct caggcgccgt tgcgcgatgc
      901 cgaagttgca aacttcgcgc acttcctgcg catccctgat cccgaactgt ctctggtgaa
      961 cctgaatact ccgctgggca cccagctgcg tcgccccctg ttcgaattct ccggcgcgtg
     1021 cgctggctgc ggcgagaccg ggtatgtcaa actgctgacc cagttgttcg gtgatcacct
     1081 gatgatcgcc aatgccacgg gttgcagctc gatctacggc ggcaacctgc ctacgactcc
     1141 ctactgctcg cgctctgatg ggcgtggtcc ggcctggtcg aacagtctgt ttgaagacaa
     1201 tgccgaattc ggcatgggta tgcgtctggc ggcggataaa atgcgcgctc aggctctcga
     1261 actgatcgac aaactgagcg cctgcacctg caaatcctgc aaagacgcac ttcctctctt
     1321 cgaggcggtg aagaaacagg atcagggaac acctgcgggg atcgaagccc agcgtgaaaa
     1381 cgtggccaag ctgaagaaag cactgcaggg gtgcaacgaa ctcgaagcca aacgactgct
     1441 gacccttgcc gactatctcg tgaagcgatc ggtctggatc atcggtggcg acggctgggc
     1501 gtatgatatc ggctacggtg gtcttgatca tgtgctggcc agtggccgca atgtcaagat
     1561 gctggtgctc gataccgaag tctattcgaa caccggtggt cagatgtcca agtcgacacc
     1621 gcttggttcg actgcgcgct ttgccgctgg tggcaaggaa accccgaaga aggacctcgg
     1681 actcatcgca atgagctacg gctacgtcta cgtggccaag gtctcgctgg gtgccaaccc
     1741 cggtcaggtc atcaaggcct tccaggaagc cgaagcctac gatggtccgg cgatcatcat
     1801 cgcctacagc cactgtatcg ctcacggcat cgaaatgagc aagggtctcg aaaaccagaa
     1861 gatggcggtg aattccggtc actggccgct gatgcgcttc aacccgaacc tgctcaagga
     1921 tggcaagaac ccgctggtcc tcgattcaca ggcgccttcg atcaagttcg aagactacgc
     1981 gatgaacgag aaccgctatc gcgtactgaa gaagagccgc cccgacgcgg tgaagaactt
     2041 catgagccag aacgaaaaga atgtgcagat gcgcttcaaa ctgtacaaat cgctggccga
     2101 aatgaacatg gaaaaactgt cctagttctt gttgagtcag agccccgcct gagtaagctg
     2161 aggcggggct ctttcatttt cggcactttc tggtaaactg ttgtggtccg tgaatcgaca
     2221 atgcctggag gttccccatg aactcgtgga caaagactcg cctggtgacg atcgtgtggt
     2281 tttttctgct ggcgtgcgct ctcggggtca gcgccgcaga aggacaactg ccggcgatat
     2341 tccagcaggg cacgtttgag gaagtctcag ctcgcgccga gaaggaacaa cgattgttga
     2401 tcgtcgattt catggcgacc tggtgccctc catgcaagcg catggatcag atgacctggc
     2461 ccgacatggc tctcatggcc tgggtggctt ctcatgcgct ggccattcag attgacgttg
     2521 ataaggaagc caaactggcc gagaatttcg ggatcgaagc catgcccacc gttctggtgc
     2581 tgaaacgaga taaaacactg ggtcggttca ccggctttcg tgacgcggat gatctgctga
     2641 cctggcttga acaggtgagg accggcaaag aacctccccc tgcgccggcg gcgaagaatc
     2701 cgcgtgaaga agggcagcgc aagatgaaag aagctcgtga agcgctggcg gcgggcaact
     2761 tcgatgccgc gaccgagcag tttctctggc tgtgggacca tctgctcgag ttgtcgccgt
     2821 catttgttgg cgtgaagtac agctttcttc tcgatgatct gaaaagcctg gcctcgaaac
     2881 atgctccggc tcgcgccaaa ttggcagcca tgcgggacaa gttgaacgcc gttgtcgaag
     2941 ccgagagggt tgatccggcg cagttcaaag actggttctc cctctgtcgt atcgtcggcg
     3001 atgatgagaa aatcatggcc tggtatgaag cgtcggccaa gagtccgagt tcattgtcgc
     3061 gtctgctgcc ggtacagctc gatatcaccg actttctgct tgagcacaag agattggccg
     3121 aagcggggag gctggtcact gatccactga cgaacgttcg cgcgatgctg ttcatgggca
     3181 aaaccggcga tcttctcgaa gatgcgagtc tggcgcggtt catcggcggg cgctggctgg
     3241 cactgctggc cgccggtcgt cgtgacgaag ccgtgcagat tcggaaagag ctcttcatca
     3301 gccttggaca ggctcccgaa gtgaagatca atctcatgga agcgggagct gacgccgggg
     3361 tgcgactgcc cgaattcgat gtctggatca aggaagcaca ggagaagagc aaggatgtgg
     3421 cgtcgttgag cgcagcgctg aaaaacctgc cgcttctgaa agtgccctga gtcgaacgct
     3481 actcttcggg ctgaagatcc gtatccggat tagtgtccgg aatcgatgag ttcgtacgct
     3541 gatgcaggcg cacgacgaac gtgctgccct tgccaacgtc gctgaccaca cgtatgtcgc
     3601 cacccatgcg atcaataaag tttttcgcca ggtacaaacc gattcccgag cctccgaact
     3661 tgcgacgaat cgtgccatcg acctggcgaa atcgttcgaa gatgatcggc tgcttttctg
     3721 gagcaatgcc gcggccgaaa tcctgaaccg agaattcaac cagatcgccg acgactccag
     3781 ctcggacaat gacccgattt ttctcgggtg aaaacttgat ggcgttgctg agcagattga
     3841 tcatgacctg cttgaaccga atcggatcga tctcgacgtg tggaacatcc gcttcgaact
     3901 gggcctggac ttcgacctga cgcgtaccgg cgaagccgct ggtgtgttcg atggcttcga
     3961 tgatcagggg aatcggatca gtgctttgca gatggactcg cattttcccg gtatcaatcg
     4021 attccaggtc gagaaggtcg ttgatcaggc tgagcagatg acggccggtc ttcttgatct
     4081 cgcgtgcgcc ggtcagcaca tggtctctca tttcgtaatg ttcgagaatg atatcgggcc
     4141 aggcaagaat cgaaacgagc ggtgtgcgca actcgtggga catgttggcg agaaactcgg
     4201 atttcaaccg gtcggactgg agtgccttct cggtctgaat ctgcagttcg tgcgttcgct
     4261 cggcaacctt ctgctcgagg gtcaggttga cgttctgcag cgcgagatgg ttccgcttga
     4321 tctcttccgc catctggacg aaggtgttgt agaaatactc gatctcctgc ggtgctgtca
     4381 cgaacgcggg cacctgatca tattcctgac gtgacaagtg ttcggcggct ttcttgatct
     4441 cgagtagagg ggtgataatc agatttccga aataccgggc tccaatgtag atgaacagca
     4501 ggccgacgag tagagcgccg atcatgatgg cgatgttggt ggtgatttca ctgttgatgc
     4561 gatcgagtga aaatccgatg gccatttcgc cgagttgtcg ctgattgaac tgcaggggaa
     4621 cttgtacatg cagtacggat ccacgatcga tgacgctggc tgttgatgcg acacccgtgc
     4681 tggttgccgg cgctcccgcc ggatgttcgc cggaaaaaga ggcaaacggt ttctgcccga
     4741 cgttctctgg ctgatacgag gcgacgacat tctggttgcg atcggtgacg atgacatacg
     4801 agacttcacc gccctgaatc aagccgttga tgacttcctg caccgctttc acgtcttcga
     4861 actcgacccc gggggccagt gtataaccgg caagacgtgc cagccgcagc gtttctttct
     4921 cgaacgctcc gcgaatcagc atggcttctc gaacattcac gaatatgcca aagacgacga
     4981 atagcgggag taccatgcag aaaaacacga tgcgcagctg ctgctgcaga cctgagcgat
     5041 tcagagactc gcggaaactc atttggtgat gatgcagtgt ttcagaaact gcgcgtgaaa
     5101 atcgaaaccc agctttcggg ccaggccgag attgaaatga atgactggct tctgatcttc
     5161 gaggcgcaac agaatcacga cgtcggaaac atcggcgtca tgcatggccg cggccgacag
     5221 gaccttgcgc tcgttggccc aggccaggag ggacgctttt tcctcatcga gctctccgtg
     5281 caaaatcagc aggtgacagg cattgagtgc tgaggtcttg aaggccttcc cgatggccac
     5341 gatgttcagg gtgatcggcc gaccaagaat ggttttgccg cccagaatgt ctttgatgcg
     5401 agccgacagt tcctcgagag actgcttccc tgccgcttta ttgtaggcga tcgtgaccgc
     5461 gaatgaatcg agtttgtcga aagacagcgt gcgattgtag gtcatgatct tcaagaagat
     5521 ctgtagctgc agatcgagag gaatctcttc ttctgatacg gcgcagcagg gcatggaggc
     5581 gcaacagaag agaatgagca gcagcactcg cgccatgacc gtgaccggtc gatggcgtcg
     5641 agacgagaga ttcagattag aagtggcgag tgtactcaca gtggactgtt gtccccggaa
     5701 ccggggctcc gatcatttct tcataggtgc cgcgaccgag gttccaggct gatagcttga
     5761 ttcgctcgcg ttcggacagt ttcaggttga gcgaggcgcc ggtaatggaa tacgcttcga
     5821 ccgtgcgtac gccgataccg ggcacgtgag ccagattcgt atctgtggtt tcggcgacat
     5881 ggtggtggac gagatgcagc gaagttcgat ggttcaaagt gtgccagatt ccggccgaga
     5941 tcatgcgcgg agcgtcgtac tgcagatcgc gttctcgact atctttcgac gacagccaga
     6001 cgaagttcga aaacagccgg gttttcggtg agaaccgttt ctgcacctcg aactcgatcc
     6061 ccttgatctt cgcgtcgtca cccggctgct gggggtgaaa cttccccagg attccgtagc
     6121 gggcatacgc ggggtcggcg acgaccgggt cgaagttttc ccagatgatg ttctcatgac
     6181 ggaacgagaa gagggtagct cccagtctga gctcgcgcgg gaaggtgtac tcgatcgcca
     6241 gctcgttcgc ctgaagcttc tcgggcgtca tatctggagt gcccttgcct gaaggctggt
     6301 cgatgtaaga ctgccagttc gacggctcct tgaaggctcg accatgcgtc agtcgcactt
     6361 tcccgcgttg gctgtcgaac gagtgcatca ggctggcgtg cgggctgaaa atattgtcat
     6421 aattcggatg tgtgtcgtaa cgtccgccaa agctgaggct ccagcgtgaa tcgaacttgt
     6481 acaaatcctg aaagaacaga ttccagttct ctttgaggat gctgcgtttg tccgggtaga
     6541 agtcatcgcc atggtacgat tcggaacgga gtccggtgcc gatggaaaga cgatgtttgc
     6601 gatactgaat gtcgcgctga aggttgaaat acagacggta ggaacggata tcgtaggtca
     6661 ggtcggggac gtcggcaaag tcgtagaagt gttcgttaaa ggcatgccag ctgaaaacaa
     6721 acgagtcttc gatcttctgc ccgcgaggct gccgaaagtg ccagtcgagc cacttttcgt
     6781 ctcgccccca ggtatacagg cgatagacgg caaagtccgc gccatcgtaa tcctgcaata
     6841 cccggcgcgc cccaacttgt actttgtggt tcccccactc atacttgccg cgcagaaacc
     6901 cccacatgcg atcggtgtgc ttggcttctc cgggacgatt gcggtcacgt accaggccgg
     6961 ggccgcgcgt ggtgccgcca tcggcggtcc acagccactt cgcccgccca ttccgccctg
     7021 cgacggccag tgaattgatg ctggtgtcga aagtgccata cgaaacctga gcctgcccgg
     7081 tgagaccgtg cttcagatag tcgtcggggg acttggtgag aatgtgtaca actccgagaa
     7141 aggcgtttga gccatacaga ggggacccgg ggccggaaag aacctcgatg cgtttgatat
     7201 tgctgagagg aatcgaggtg atgtcggtcc agataccgcc gtcgcgcacg tcgttgatcg
     7261 gaacgccgtc gaccatccag aggatacgtt cattgataac tgaggcgccg agcattccgc
     7321 gtatggaggt gttcgtaaac tgcgacagcc aggtctttga ctgaaatccg ggtaccatcc
     7381 gcagaagatc ggcaagagtg atggcagggg agaggcgaat ctcttcctca gtgatgacat
     7441 gggtggtcat cggcgagtcg ctgagccgct gttcatgacg tgccgcggtg acgacgacct
     7501 ggttcagcag atcatcgaga gacacttccg aaaaatcctg aaagccggtg ctgctggcat
     7561 tcgcggcaat ggccgcgtgg ttggcacagc tgcagatacc ggcgataatg cagaaaacgg
     7621 ctccccaacg gcgtaaatgg gaggatgcgg ttttgtcagt catggcaatt cctctcgaac
     7681 gtttttagtg gatgaaaacg atagcataaa aaccattcag gagaaaccct gaacgg
//
LOCUS       DAORHD010000043         7682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9153_length_7682_cov_3.918972, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000043 DAORHD010000000
VERSION     DAORHD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9153_length_7682_cov_3.918972"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PKO06_01725"
     CDS             complement(<1..533)
                     /locus_tag="PKO06_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HNV68387.1"
                     /translation="MKIAFHPIPESERKPIPADPQKLGFCKVFTDYMFIMQYSEEEGG
                     WHTPEIRKNAPLPLEPSTIGLHYAQTIFEGLKAFRTSRGTIQLFRPEMNAKRFNRSAA
                     RLCMPAIPEYDFLESIEALIRVEERWVPRSRGAALYVRPFMVASDTTLGVRAASNYLY
                     MVILSPVGPYYQEGFNP"
     gene            716..1105
                     /locus_tag="PKO06_01730"
     CDS             716..1105
                     /locus_tag="PKO06_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV68388.1"
                     /translation="MKGITPFGIYTNHPEESRNFYRSVLGIERIWSMSAAEQAAERSG
                     VVLRLESPRLELFASELRLTDRRVNDRVPGLCQLVLYPGDLLRAAQTLEKAGVDYRAD
                     YTGRLYFEDLNGIQWEMRETPGTAGLT"
     gene            complement(1098..1454)
                     /locus_tag="PKO06_01735"
     CDS             complement(1098..1454)
                     /locus_tag="PKO06_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006518648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV68389.1"
                     /translation="MIYLAYLAWGAAIGILSSMIGLGGGVLIVPTLIWLFGLTQHQAQ
                     GTSLALMIPPIGLLAAYKYWLHGNVILPIAICGALGFFLGGYLGADLAHWMNPQQMRR
                     LFGIVLVTIGISFLVT"
     gene            complement(1508..1732)
                     /locus_tag="PKO06_01740"
     CDS             complement(1508..1732)
                     /locus_tag="PKO06_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68390.1"
                     /translation="MATLFGYSTYRKILQYDLKLATIHNFQKKTPTFEGVLAWTIDKE
                     DEMRDLIKLGVIGILTNRPDTLSRVVTTWK"
     gene            complement(1678..2541)
                     /locus_tag="PKO06_01745"
     CDS             complement(1678..2541)
                     /locus_tag="PKO06_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNV68391.1"
                     /translation="MRLSASRSPRVGLCLLLIVVWAVFAPLAGHSQDLRLQKPGADFL
                     IVGHMGAPLHAVENTLPSFQKALELGANAIETDVCITKDGEVVLWHDWSPHATVSLFR
                     QLGKQGLKARPFNPDLGSAFRRPVNELTLAELRQHFGYALRQTRIGENKRLPDRIVTI
                     REFAQWAANRDDLVKVLFDIKVPAKNSAWVRPLMKRICSVLNQYNLLDRAAFMSPEPA
                     ILDVIADMNDLPEITVVHDQELPPASSFIRRSFPPFRKRSTAIFPVLRSDDRWRHCSG
                     IPPIARFFSMT"
     gene            complement(2628..3953)
                     /locus_tag="PKO06_01750"
     CDS             complement(2628..3953)
                     /locus_tag="PKO06_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV68392.1"
                     /translation="MLVNMWDKVVAAIGQKNVAEVRQLLRQAPELAIQKNYQDETLLH
                     FAANVGSPEIVADLLVRGAKPDLPDEFGWTPLHEACGNGNEAIVAMFLQLPFNLDGVS
                     RKGETALHLATRQGFAKIVKQLLQAGANHEFRNRDGNTPLHVAALAGRLDLLQIIREA
                     GGDIHARNLNGETPLHSCAREGHIEAALWCLSHGANADEQDKGYRTFIDTAIREGRQL
                     FVQHFAELEQPVPTPDNPNPLAATGLRLDVTINLKTMATQARKPGGKLFRAYLFGVPY
                     MTYRMMLGALLEAALWFFVFPFLIFLLFAGLAQGLLPRVIPVPGTWSAELLPIVGAGL
                     NTLVFFIASHLFTTPPEGRRLERNALRRFRSTVWVRLLHFAVPALLTIRSLTYDQLFW
                     RQLSLFWLWFMMLYGLLFIGWWVGKETPVEPTKPPTTPDPPLRFGSSTP"
     gene            complement(4001..>7682)
                     /locus_tag="PKO06_01755"
     CDS             complement(4001..>7682)
                     /locus_tag="PKO06_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV68393.1"
                     /translation="AAELFQLVFGRAKAPSLPAGSGRLEDWVIGGNYRVLNRMGSGGT
                     ASVLRATDLSLNREVALKILLENETTDEELTKRFLAEGKILASLRHPNVVMVYSLGLD
                     QRLKFPFLVMEMVHGATLDDHLEDLRNPLLLFREMITILEAIHFCHQKKIIHLDLKPS
                     NVMIDQNGQIKIIDFGIAIPMQTQKENSFALGTAYYMAPEQFEFDRTFTPETDVYALG
                     IMLWELLTGKVPFSEPAGSADPFDALEQAHRHAEPPFESLEAIPHARALVPLLRRMLA
                     KPPAQRPAIPEIIQLLRQEMLSQGGQIGDRFEVREELHRSPLTRILSGFDRTFQQKVV
                     IEILTAEAGTRHDQMELFLQRAGRLTRLGHPCFSRVVALDREPATRLPYLVQEMAGGL
                     TLADALSLREGDAPRLLRLLLRVLLGLWRAHEAGFGHGLLGLETILVSDDDEVAFIRT
                     HNVLSDHAAITADVQAIPSLGLAFVQACTAAGMDKDDHPDYIEKARTLCTTWKAAIDE
                     SRSIDLRQMSVDVERLLINHHLEGAEPVNGAVPPLGKTPSEKLARIKRLVDVGNPVSI
                     RILLDALTGEKDVRVLAAILPALARLERRSELPVVVPFLDHPDSTVRLAAIEAIHLAG
                     AVGACLHLFDLLDDADKRVRTHAASTLKNFGDAFLKEQLQTFALFGLPDQQIQALRAL
                     RFFPSEEHLSLIELMSRSENPEIAKAARDARLTILTSELELLESTNAPPPVALRRIES
                     MENHTIVCGYGRKGQMIVQSLLARDEHCVLIENNDQQSGLELARAQGIPTLVGSATHA
                     PLLLAAGVTRARQVFAVTNDDDVNLEIALKTKMLLGNLLRPRHLEPLQCLTHVFSISL
                     ACMADLFAPLRTGTRDRFQVLSTAPGFELRLFNIYENAARQILAGNLPSVSGLQDQLH
                     GKPLHVLILGVTNLGVNLAVRVATQLQGPDPGLLKITIVDQSRHLREAAIRFHYPNID
                     RIVSLNLVPLAEKDLTPEAIERLAGFDPFAAIFVCHQNQNLGQKLGAMVRQFPSVSAH
                     VPMLVVGRAATPAATSTPSAPSGNPATAQNQFARFDLFSATCQVDAILQEPLDQLAHA
                     IHQQFCEGDTIEPNDPRRVPWERLRADVKEHFRAQADHLAVKARTLGCALVAHQEPGR
                     AYVMSDDDVGTLARLEFRRRVVESQFSSSHGASFLETLRETGKDAPTWDVLPQPEREE
                     LYRQIRGIPTLLASIGQKMQRL"
BASE COUNT         1631 a         2127 c         2403 g         1521 t
ORIGIN      
        1 atcgggttga atccttcctg atagtagggg ccgaccgggc tgagaatgac catgtacagg
       61 taattggaag cggcacgcac accgagagtg gtatcggaag ccaccatgaa cggacgcaca
      121 tacagagccg caccgcgact gcgcggaacc cagcgttctt cgacgcggat cagggcctcg
      181 atactctcga gaaaatcata ctcgggaatg gcgggcatac agagtcgcgc ggctgagcga
      241 ttgaagcgct tggcattcat ttcgggccga aagagctgaa tcgtgccgcg agacgtgcga
      301 aaagccttca agccttcaaa aatggtctgt gcatagtgta gaccgatcgt cgaaggttcg
      361 agcggaagcg gggcattctt gcggatctcg ggggtgtgcc aaccgccctc ttcctccgaa
      421 tactgcatga tgaacatgta gtcggtgaag actttgcaga accccagttt ctgcgggtcc
      481 gccggaatcg gtttccgttc cgattcgggt atcggatgaa aagcaatttt catggcgtct
      541 tcctttcctg gtggcctgta tttatgcgaa aagcctgtaa taaagactgc ctgcacgaga
      601 cattatactc tccacccctg gatggtgcaa taaaacccgg ccgatttctc gggtggggtt
      661 cggcgaaggg gggctcgttg ccgataagaa atcagaacct gctggaggcg catgcatgaa
      721 aggaatcacc ccgttcggta tctacacgaa tcatcccgaa gagagtcgga acttttaccg
      781 ctctgtcctg ggaatcgagc gtatctggag catgtcggcc gcagaacagg ctgccgaacg
      841 atcgggggtt gttctgcgtc tcgaaagccc gcgtctggag ctatttgcca gtgaactacg
      901 actcaccgac cgacgggtca atgatcgggt tcccggcctt tgccaactgg ttctctaccc
      961 gggtgatctg ctgagagcgg cacagacgct ggaaaaggcg ggagtcgact atcgcgcgga
     1021 ctacaccggg cgactctact tcgaggatct gaacgggatt cagtgggaga tgcgcgagac
     1081 gccaggcact gccgggctca cgtgacgaga aaactgattc cgatcgtgac cagcacgatg
     1141 ccgaacaggc gccgcatctg ctgtgggttc atccagtgcg ccagatcagc gcccagataa
     1201 ccgccgagaa agaagccgag cgctccacaa atggcaatcg gcagaatgac gttgccgtgc
     1261 agccagtatt tgtaggcggc cagcaggccg atggggggaa tcatgagcgc gagagaagtt
     1321 ccctgggctt gatgttgcgt caggccgaac aaccagatca gcgtcggaac gataagcacg
     1381 ccgcccccca ggccaatcat gctcgacagg attccgatag cggcacccca agccaaatag
     1441 gcgagataga tcatgctgcg attctacgaa attgggcttg tgactgtcca gtgtcagtca
     1501 cgcgtgtcta cttccaggtg gtgacgactc gcgacagcgt gtccggacga ttggtgagaa
     1561 tgccgatgac gccgagcttt atcagatcac gcatctcgtc ttccttgtcg atcgtccagg
     1621 cgaggacacc ctcgaacgtc ggcgttttct tctgaaagtt atgaatggtg gccagtttca
     1681 ggtcatactg aagaatcttg cgataggtgg aatacccgaa cagtgtcgcc accggtcgtc
     1741 cgatcgaagc acaggaaaga tggcggttga gcgctttctg aacggtggaa aacttcttcg
     1801 gatgaatgat gatgccgggg gaagttcctg gtcgtgaacg accgtgatct cgggcagatc
     1861 gttcatgtcg gcgatgacat cgagaatcgc gggctcaggt gacatgaacg cggcccggtc
     1921 gagaagattg tactggttga gaactgagca gatgcgtttc atgagcggtc tgacccaggc
     1981 gctgttttta gcggggacct tgatgtcgaa gaggactttg acgaggtcgt cgcggttggc
     2041 ggcccattgc gcgaactctc tgatcgtgac gatgcggtcg ggcagccgtt tgttctcacc
     2101 gatcctggtc tgacgaaggg catacccgaa gtgttgccga agttccgcga gggtgagctc
     2161 attcaccggc cgtcggaaag cgctgccgag atcaggattg aacgggcggg ctttgagccc
     2221 ctgtttgccg agctgcctga agagtgagac cgtggcgtgg gggctccagt cgtgccacag
     2281 aacgacttct ccgtctttgg tgatgcagac atcggtttca atcgcgttgg cacccagctc
     2341 gagagccttc tggaaactgg gcagggtgtt ttccacggca tgcaggggag cgcccatgtg
     2401 cccgacgatc aggaaatccg caccaggttt ctgaaggcgc agatcctggc tgtgaccggc
     2461 gaggggggcg aaaaccgccc acacgacgat cagcagaagg cagagaccga cgcgtggact
     2521 tcgagaggcg gagagtctca tatgcagtca tctcctgaaa tgccgtcagt acctctcatt
     2581 atacgaagaa gaggcacttt cggcaaaaac cgcttgcaga gcggtgatca gggggtcgaa
     2641 gagccgaatc gcagcggtgg atcaggggtt gtcggaggct tcgtgggttc gacaggcgtc
     2701 tctttgccga cccaccagcc gatgaacagc aggccgtaca acatcatgaa ccagagccag
     2761 aacaggctca gctgccgcca gaacaactga tcgtaggtga gcgaacgaat agtcagcagc
     2821 gcgggcaccg cgaaatgaag cagtcgcacc cagacggtgc tgcgaaagcg gcgcagggca
     2881 ttgcgttcca gacgtctgcc ctcagggggc gtcgtgaaca gatgagaggc aatgaagaag
     2941 acgagggtgt tgagtccggc cccgacaatg ggcagcagct ccgcggacca cgttccggga
     3001 acgggaatga cgcgtggtaa caagccctgt gccagcccgg cgaacagcag aaaaatcaga
     3061 aagggaaaga cgaagaacca cagggctgct tcgagcagcg cgccgagcat catgcgatac
     3121 gtcatgtacg gcacgccgaa cagataggcg cgaaagagct tgccccccgg tttgcgcgcc
     3181 tgagtggcca ttgtcttgag attgatcgtg acatccagac gcagaccggt ggccgcgagc
     3241 ggattcgggt tgtcgggcgt cggaaccggt tgttcgagtt cggcaaaatg ctgcacgaac
     3301 aactgacgcc cttctcgaat cgcggtatcg atgaacgtgc gatacccttt gtcctgttca
     3361 tcggcgttgg ctccgtgcga gaggcaccag agcgcggctt cgatgtggcc ttcgcgggcg
     3421 caggaatgca gcggtgtttc cccgttcaga ttacgggcat gaatgtcgcc accggcctca
     3481 cgtatgatct gcagcaggtc taaacgccct gccagagcgg ctacatgcag gggagtgttg
     3541 ccgtctcgat tgcgaaactc atgattggct ccggcctgca gcaactgctt cacgatcttg
     3601 gcgaaaccct gtcgggtagc gagatgcagg gctgtctccc ccttgcgcga tacgccgtcg
     3661 aggttgaatg gcagctgcag aaacatggcc acaatggctt cattgccgtt gccgcaggct
     3721 tcatgcaagg gcgtccagcc gaattcgtcc ggtaggtcag gcttggcacc acgtaccagc
     3781 aggtcggcga cgatctcggg tgacccgacg ttcgccgcga aatgcagcag cgtttcgtcc
     3841 tggtagtttt tctgaatcgc cagttctgga gcctgccgaa gcagttggcg gacttccgcg
     3901 acgttcttct gaccaatggc ggcaacgact ttatcccaca tatttacgag catatcacag
     3961 ctgctggcgg ggagcgaatt ctttcccgcg gccggaaggc tcagagtcgt tgcatctttt
     4021 gcccgatcga agccagcagg gtgggaatgc cgcgaatctg tcggtacaac tcctcgcgtt
     4081 ccggctgggg caagacgtcc caggtcggcg cgtcttttcc cgtctcgcgt aacgtttcga
     4141 gaaagctcgc tccatgagag gaggaaaact gcgattcgac cacccggcgc cggaactcaa
     4201 ggcgtgcgag cgtgccgacg tcgtcatcgc tcatgacgta ggcacgtccc ggctcctggt
     4261 gggcaaccag ggcgcagccg agtgttctgg ccttcacggc caggtgatca gcctgggcgc
     4321 gaaagtgttc cttgacgtcg gctcgcaggc gttcccacgg cacccgtcgc ggatcgttcg
     4381 gttcgatggt gtccccttcg cagaactgct ggtgaatggc gtgagcgagc tggtcgagcg
     4441 gttcctgcag aatcgcgtcg acttgacagg tggccgaaaa gagatcgaat cgcgcgaatt
     4501 gattctgagc ggtggcgggg ttcccggatg gcgctgatgg cgtggaagtc gctgctggtg
     4561 ttgctgcgcg cccgaccacc agcatcggaa catgcgccga caccgacgga aactgccgca
     4621 ccatggcacc gagcttctgc ccgagattct gattctggtg acacacgaag atagccgcga
     4681 atggatcgaa ccctgccagg cgttcgatcg cctccggtgt cagatctttc tccgccagag
     4741 gcaccagatt cagggaaacg atacgatcga tgttggggta atgaaagcga atggccgcct
     4801 ctctcagatg acgtgactga tcgacaatcg tgatcttcag aagtccggga tcaggccctt
     4861 gcagctgagt tgcgactcgt actgccagat tgaccccaag gttggtaacc ccgagaatca
     4921 gcacgtgcag cggtttgccg tgtagctggt cctgcagacc ggaaaccgat ggcaggttcc
     4981 cggcgagaat ctgccgggcg gcgttctcgt agatgttgaa caaccgcagt tcgaatccgg
     5041 gcgcggtcga cagcacctga aagcgatctc gagtaccggt tcgcaggggt gcgaacaggt
     5101 cagccatgca ggcgagcgaa atcgaaaaca cgtgcgtcag acattgcagg ggctcgagat
     5161 ggcgcggacg cagcaggttt cccagcagca tcttggtctt gagggcaatt tcgagattca
     5221 cgtcatcgtc gtttgtgacc gcgaaaacct gacgggctcg ggtcacgcct gccgccagca
     5281 gcagcggtgc gtgggtcgct gaaccgacca gagtcgggat cccctgagcc cgcgccagtt
     5341 ccagcccgct ttgttggtca ttgttctcga tcagcacgca gtgctcgtca cgcgccagaa
     5401 gactctggac aatcatttgc cccttccgac cgtagccgca cacgatcgtg tgattctcca
     5461 tcgattcgat tcgccgcaga gcgaccggag gcggggcgtt cgtggattcc aggagttcca
     5521 gttccgaggt caggatagtc agacgcgcat cacgcgcggc ttttgcaatt tcaggattct
     5581 ctgaacggct catcagttcg atcagcgata aatgttcttc gctgggaaag aagcgcagcg
     5641 cgcgtagagc ctgtatctgc tgatccggca ggccgaagag cgcgaacgtc tgcagttgtt
     5701 ccttcaggaa cgcgtcgccg aagttcttca gtgtcgaggc ggcgtgggtg cgcacgcgtt
     5761 tgtccgcatc gtcgagcagg tcgaacaggt gcagacaagc cccaaccgct ccagccagat
     5821 ggatcgcttc aatggcggcg agacgaacgg tggagtccgg atgatccaga aatggcacca
     5881 ccacgggcaa ctcggaacgt cgctcgaggc gcgccagagc cggcagaatc gccgccagaa
     5941 ctcggacatc cttctcgccc gtcagggcgt cgagaaggat ccggatactg accgggtttc
     6001 cgacatcgac gaggcgtttg atgcgtgcga gtttctctga aggagttttc ccgagggggg
     6061 ggacagcgcc gttgactggt tccgcgccct ccagatgatg gttgatcagc agccgctcga
     6121 catccaccga catctgtcgc agatcgatgg accgtgactc gtcgatggcg gctttccagg
     6181 tggtacagag ggttcgggct ttctcgatat aatcggggtg gtcgtccttg tccataccag
     6241 ccgcggtgca ggcctgaaca aatgcgagac ccagcgacgg tatggcctga acatccgccg
     6301 tgatggcggc gtggtctgaa agaacgttgt gggtgcgtat gaacgccacc tcgtcatcat
     6361 cagaaacgag aatcgtctcg agtccgagaa gcccatgacc aaaaccagct tcatgcgctc
     6421 tccagagccc cagaagaacc cgcaacagaa gtcgcagcag gcggggggcg tccccttcac
     6481 gaagactcag ggcgtcggcg agcgtcagac ctccggccat ctcctgcacc agatacggca
     6541 gtcgtgtggc gggttcgcgg tcgagagcga ccacacgcga aaagcacggg tgtccgagtc
     6601 gtgtcagccg ccctgcgcgt tgcagaaaca actccatctg gtcatgacgg gttccggcct
     6661 ccgcggtcag aatctcgatg accaccttct gttgaaatgt tcgatcgaat cctgacagaa
     6721 tgcgggtgag cggactgcga tgcagttctt cccgaacctc gaaacggtcg ccgatctgac
     6781 cgccctgcga cagcatttcc tgccgcagga gttgaatgat ttccggaatg gcgggtcgct
     6841 gcgcgggcgg tttggcgagc atgcgtcgca gtaatggtac gagtgcgcgc gcgtgaggaa
     6901 tggcttccag agactcaaac ggcggctccg catgacggtg cgcctgctcg agggcatcga
     6961 atgggtccgc gcttccggcc ggttcgctga aggggacttt gccggtgagc agctcccaga
     7021 gcatgatacc cagggcgtag acatcggttt ccggggtgaa ggtgcgatcg aactcgaact
     7081 gctccggtgc catgtagtag gcggtaccca gagcaaacga gttctccttc tgggtctgca
     7141 tgggaatggc aatgccgaaa tcgatgatct tgatctgacc gttctggtca atcatcacgt
     7201 tcgagggctt caggtcgaga tgaatgattt tcttctgatg acagaaatga atggcctcga
     7261 gaatcgtgat catctcgcga aacagcagca gagggttgcg caagtcttcg agatgatcgt
     7321 cgagcgtggc cccatgaacc atttccatga cgagaaacgg aaacttgaga cgctgatcga
     7381 gaccgagcga atacaccatg acgacattgg ggtgccgcag actcgcgaga atctttcctt
     7441 cggcgagaaa tcgcttggtc agctcctcgt cggtcgtctc gttttcaagc agaatcttga
     7501 gggcgacttc gcggttgagc gacagatccg tggctcgcag aacactggcg gtgccgcccg
     7561 aacccatgcg gttgagcacc cggtaattgc cgccgatgac ccagtcttcg agacggcccg
     7621 accccgccgg cagcgatggc gctttggccc gaccgaagac gagctgaaag agttccgcgg
     7681 cg
//
LOCUS       DAORHD010000044         7602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9297_length_7602_cov_3.819133, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000044 DAORHD010000000
VERSION     DAORHD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9297_length_7602_cov_3.819133"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PKO06_01760"
     CDS             complement(<1..760)
                     /locus_tag="PKO06_01760"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68394.1"
                     /translation="MTDQETRLLREQLTSTKAETRKKAFAELKKFSADRALSVLTSCL
                     DAKNSDVQADLMKAIITYKDEALPHLVKAFNDPLWRVRKTAAEIIGSMGDRALSRFLA
                     LIPQNEEDVDFWMVQTLGFMGGEATPYLMRAFHHPNPRIRLAAVRAAANTGDPQIMPS
                     LIGMLNDPSWSIRKAAYDTLSRIQHLNPEAVIEAIKRADIEAKYWLIRLAGERQDSRL
                     IPVFCGIIEHDREEAKLEAIRALAEIQRPEAHKVL"
     gene            complement(812..1945)
                     /gene="dnaN"
                     /locus_tag="PKO06_01765"
     CDS             complement(812..1945)
                     /gene="dnaN"
                     /locus_tag="PKO06_01765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNV68395.1"
                     /translation="MMKFKVDLKEFAEGLSLVGRAVAVKGVRAILSNVLVLAGENQLQ
                     LVGTDQEIMALARVPAQVETHGQCTIPAKLLQEVISNLPVDEAEGLITLEQPADNEGL
                     IHVMSRRGKYHLNVQGTEEYPPFPILEREDFPRAKVSGTALAEAMKQVAIAVGAEEAN
                     AVQRSMCFLFQGGELRLVATDSKRLAVTVLRGVTVPKELDKTFLVPGRAVSEVLKMAE
                     STPDLSIGLFKEQLVFLSEKHQFLTRLLEGRFPDYQRVIPKECSRKATFDVKSLVQSI
                     KAVLPIARQNSFMMSLDMGRNETRVWSQSKNEGTSEMFISSELNGEAIQIAFNARYLL
                     DFLGAVNAEKVSLEMTTPSYPGLFKPVADDNPYQYVVMPISML"
     gene            complement(1942..5091)
                     /gene="ileS"
                     /locus_tag="PKO06_01770"
     CDS             complement(1942..5091)
                     /gene="ileS"
                     /locus_tag="PKO06_01770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014425420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNV68396.1"
                     /translation="MLFQKTSTSISPKREEEILEFWRKEDIFRQTIKNRDGCPDFVFF
                     EGPPTANGTPHPGHVLTKAMKDLVPRYKTMCGCRVGRKAGWDTHGLPVELEVEKQLGI
                     SCKQDIEKFGIENFIKKCRESVFKYEAEWRRMCERGGFWVDMDDPYITCTDQYIETGW
                     WVLGQLWKKGMLYEGHKVVPYCPRCGTALSSHEVAQGYDEAEDPSVYVRFKIKNQADT
                     YFLVWTTTPWTLLSNVALAVGRDIDYAKVKHQGQTLIMAAARVEPVLQKDFEIVGTVK
                     GAELLGVEYEQMYTFVAPKKRAHYVIGADFVSTEDGTGIVHIAPAFGEDDYRVGQAND
                     LPVMQPVNLDGKFTDEVTPWKGRFVKDADKDIIKDMKAKGILLSSGTIKHTYPFCWRC
                     DSPLLYYARHSWFIKTTAFKEEILANNDKINWFPDHIKHGRFKNFLDNMIDWAISRDR
                     YWGTPLPIWQCECGKQECIDSREMLCRLGKNVDPKIELHKPYVDDITLDCPDCGKQMK
                     RVPEVIDCWFDSGIMHTAQWHYPFENKETFARQFPADFICEAVDQTRGWFYSLLVTST
                     FLHQAPAYKNVVVLGLICDKNGVKMSKSKGNVVDTKALFEKYGADAVRWSLYSGTAPW
                     NTRRFYEEAVSDAVSRFLGTLQNVYSFFVLYANTDNLNPTQFNVPVAERSELDRWIIS
                     RFNNTAKRVRETLDIFEITHATQALETFVDELSNWYVRRSRRRFWASELTADKKSAFR
                     TLYEVLVGTAKLIAPFTPFLAEEIYRNLVCSVDAKAPRSVHLTDYPVADVSCVDEALE
                     KNMGFARTVVQLGRAARSEANLKVRQPLADMKVVAPESWQQEVLKRMQAIIQEELNVK
                     QVVPVAAADDLVKCVIKPNFRTLGKGPFKGLIPKIKAHFEQADGNTVQKALAAGGYVC
                     LLDGQEVKLTAEDVEVSKTASGDYAVAAEGTLTVALNQILTRPLVLEGMARELVNKIQ
                     FMRKEQNFDIADRIRVAYRLGEHANAGDLGEALQQHGDYIRGETLALEIAAADHIQGT
                     EWNINGIPIRLEISKESKS"
     gene            complement(5136..5732)
                     /locus_tag="PKO06_01775"
     CDS             complement(5136..5732)
                     /locus_tag="PKO06_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNV68397.1"
                     /translation="MNVLRDFQQVGADLFAARLNNTHSGNLSLRLNRMLAITRTGSML
                     HRLGHNDVVETLIDGDDLETPRASREIPVHRAIYRATGAQAVVHAHPPHVIALSLLVD
                     RIMPLDGEGRYYFPNGIPILTVDNPVGSEEVARGLPPLLKTSPIVVVRGHGTFAIGTD
                     LESGLHWTSSLDNIAQIVLLSRSGMHSLLASPIDRPRS"
     gene            complement(5729..6838)
                     /gene="mtnA"
                     /locus_tag="PKO06_01780"
     CDS             complement(5729..6838)
                     /gene="mtnA"
                     /locus_tag="PKO06_01780"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNV68398.1"
                     /translation="MQKELPMIPTVQYQPETDTVSLLDQTRLPCERVMMPCASVDQMC
                     EAIRALRVRGAPAIGVAAAFGLYIGLREYSGSADGVAAHLEAVATRLAETRPTAVNLF
                     WALNRASKAIQQQIAAAPAAQRLKAARDEALRQATLIMQEDIDMCRAIGFYGAAYLLN
                     GDTWLTHCNAGALATGGYGTALGVFRAAKEQNKRFSVFVDETRPLLQGARLTAWELLE
                     EGIDATLICDNMAASLMAAGKIQGIVVGADRITAEGWVANKIGTYGLAVLAHYHQVPF
                     YVAAPVSTFDLGVRHGREIVIEQRSADEVAKWGGIQTAPTGMPVYNPAFDVTPPDLVT
                     AIFTNLGVLKAPYAQSIHETLHGAGLDHLHGVKRP"
     gene            7053..7346
                     /locus_tag="PKO06_01785"
     CDS             7053..7346
                     /locus_tag="PKO06_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNV68399.1"
                     /translation="MSVKLFVGNLPFSTDDNRLKEEFTKFGAVESARVVTDKHTGRSR
                     GYGFVEFSDQTAADAAVTGMNGQNMDGRPLTVSVAKSQGTGPNGPRRERSQDM"
     gene            complement(7390..>7602)
                     /locus_tag="PKO06_01790"
     CDS             complement(7390..>7602)
                     /locus_tag="PKO06_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68400.1"
                     /translation="LVQGAQQIHAVHAMVDGGLQYPLDQGTFAAIHLCVQEVREKAQV
                     KVSRHESECEKQKHPQPPDERAVEGK"
BASE COUNT         1506 a         2255 c         2197 g         1644 t
ORIGIN      
        1 tcagcacttt gtgggcttcg ggacgctgaa tctcagccag ggcccgaatc gcttcgagct
       61 tcgcctcttc gcggtcatgt tcgatgatgc cgcaaaacac gggaatcaac cgggaatcct
      121 ggcgctcccc ggccagacgg atcagccagt atttggcttc tatgtcggcc cgcttgatgg
      181 cttcgatcac cgcttcaggg ttgagatgct gaatccggga aagcgtgtcg taggccgcct
      241 tgcgaatcga ccacgaggga tcgttgagca tgccgatcag cgacggcatg atctgcggat
      301 cgccggtatt ggccgcggca cgcacggcgg cgaggcgaat gcgcggattc gggtggtgga
      361 acgcacgcat cagatagggt gtcgcttcgc cgcccatgaa gccgagcgtc tgcaccatcc
      421 agaagtcgac gtcttcctca ttctgaggaa tgagcgcgag aaaccggctg agggcccggt
      481 cgcccatact gccgataatc tcggcggccg tcttgcgcac acgccacaag gggtcgttga
      541 aggccttgac cagatgcggc agcgcttcat ctttgtaggt gataatcgcc ttcatcaggt
      601 ctgcctgaac atcgctgttc ttggcatcaa gacacgaggt cagcacgctc agtgcccgat
      661 cggcgctgaa ctttttcaac tcggcgaacg ccttcttccg cgtttcggct ttggtactgg
      721 tcaactgctc gcgcaggagt ctggtttcct gatccgtcat gatccgctcc ttttccccat
      781 cacgggtact ttccatgtgc tcggcataag tttacaacat gctgatcggc atgaccacat
      841 actgatacgg gttgtcgtca gcgaccggct tgaacaagcc gggatagctg ggggtggtca
      901 tctcgaggct gaccttctcg gcattcacgg caccgagaaa gtcgagcaga tagcgcgcgt
      961 tgaaggcgat ctgaatcgct tctccattga gttcggaaga gatgaacatc tccgaggtgc
     1021 cttcattctt cgactgagac cagacacgag tctcgttgcg gcccatgtcg agcgacatca
     1081 tgaagctgtt ctgtcgcgcg atcggcagaa ccgccttgat cgactgtacc agcgacttta
     1141 cgtcaaaagt ggcttttcgg ctgcactctt tgggaatgac tctctgataa tccgggaacc
     1201 gtccctcgag caggcgggtc agaaactggt gcttttcgga cagaaacaca agctgttcct
     1261 tgaacagacc aatggagagg tcgggagtcg actcggccat cttcaacacc tcgctgaccg
     1321 cccgtcccgg aaccaggaac gtcttgtcca gttctttcgg aaccgtcacc ccacgcagca
     1381 ccgtcaccgc cagacgttta ctgtccgtcg ccaccagtcg caactctccc ccctggaaca
     1441 gaaagcacat gctgcgttgc acggcattgg cctcttccgc gccaacggca atagccacct
     1501 gtttcatggc ttccgcgagc gctgttcccg acactttggc acgcggaaaa tcttcacgtt
     1561 caagaatcgg aaacggcgga tattcctcgg ttccctgaac attcaggtgg tacttgccac
     1621 ggcggctcat cacgtgaatc aacccctcgt tgtccgcggg ttgttcgagc gtgatcaggc
     1681 cttcggcctc atcgaccggg aggttactga tgacttcctg cagcagtttg gcaggaatcg
     1741 tgcactgccc gtgcgtttcg acctgcgccg gtacccgggc cagcgccatg atttcctgat
     1801 cggtgccgac gagctgcagc tggttttctc cggcgagaac cagaacattc gaaagaatcg
     1861 cccgcacgcc cttcacggcg accgcgcgac caaccagact caggccctcg gcaaactctt
     1921 tcagatcgac cttgaacttc atcatgactt ggactccttg gaaatttcaa gccggatggg
     1981 aatcccgttg atgttccact ccgttccctg tatgtgatcc gccgccgcga tttccagcgc
     2041 cagcgtttca ccgcgaatgt agtctccatg ctgttgcaga gcttcgccca gatcaccggc
     2101 gttggcgtgc tcacccagac gataggccac ccggatgcga tcagcgatat cgaagttctg
     2161 ctctttgcgc atgaactgaa tcttgttcac cagttcgcgg gccattcctt cgaggaccag
     2221 cggccgcgtg aggatctggt tcaacgccac ggtcagcgtt ccctcagccg cgacggcata
     2281 atcgcccgac gcggtcttgc tgacctcaac gtcttccgcg gtcagcttca cttcctgtcc
     2341 atcgagcaga cagacataac cgccggcggc cagcgctttc tgcacggtat tgccgtcggc
     2401 ctgttcgaag tgtgccttga tcttcggaat cagacccttg aaggggccct tgcccagcgt
     2461 gcggaagttc ggcttgatga cgcatttgac caggtcatcg gcggcggcca ccggcaccac
     2521 ctgctttaca ttcagttctt cctgaatgat cgcctgcatg cgcttcagaa cctcctgctg
     2581 ccaggactcg ggcgctacga ccttcatgtc ggcaagcggc tggcgcacct tgagattggc
     2641 ctcgctgcgc gcggcacgcc ccaactgcac gaccgtgcgc gcaaacccca tgtttttctc
     2701 gagcgcctcg tcgacacacg acacatccgc caccgggtag tcggtgagat gcacgctgcg
     2761 cggggctttg gcatcgacgg aacacacgag gttgcgatag atttcttcgg ccaggaacgg
     2821 ggtaaacggg gcaatcagtt tggccgtgcc gaccagcacc tcgtagagcg tgcggaacgc
     2881 ggactttttg tcggcggtca gttccgacgc ccagaagcgg cgacgactac gacgaacgta
     2941 ccagttcgac agctcgtcaa caaacgtttc aagagcctga gtggcatgcg tgatttcgaa
     3001 gatgtcgaga gtttcacgca cgcgtttcgc ggtattgttg aagcgtgaga tgatccagcg
     3061 gtcgagttca ctgcgttcgg ccactggcac gttgaactgc gtcgggttca aattgtccgt
     3121 gttcgcatag agcacgaaga agctgtagac gttctgcagc gtacccagaa accggctcac
     3181 ggcatcagaa acagcttctt cgtagaaacg acgggtgttc cagggcgcgg tgcccgaata
     3241 gagcgaccag cgcaccgcat cggctccgta tttctcgaac agggccttgg tatcgacgac
     3301 attccccttc gacttgctca tcttgacgcc gttcttgtcg cagatgagcc cgagcacgac
     3361 gacgttcttg taggccggag cctggtgcag gaaggtgctg gtcaccagca gactgtagaa
     3421 ccacccccgg gtctggtcga ccgcttcaca gatgaagtcg gcggggaact gccgcgcgaa
     3481 ggtttccttg ttctcaaagg ggtaatgcca ctgggccgtg tgcatgatgc ctgagtcgaa
     3541 ccagcagtcg atgacctcgg gcacgcgctt catctgtttt ccacagtcgg ggcagtccag
     3601 ggtgatatcg tcgacatacg gcttgtgaag ctcgatcttc gggtcgacgt tcttcccgag
     3661 ccgacacagc atctcgcgac tgtcgatgca ttcctgtttg ccgcactcgc actgccagat
     3721 cggcagcggg gtgccccagt agcgatctcg cgagattgcc caatcgatca tgttgtcgag
     3781 aaaattcttg aatcgcccgt gcttgatgtg atccgggaac cagttgatct tgtcgttgtt
     3841 ggcgagaatc tcttccttga aagccgtggt cttgatgaac cacgaatggc gtgcgtagta
     3901 gagcaacgga ctgtcgcagc gccagcagaa cggatacgtg tgcttgatcg tgcccgacga
     3961 aagcagaatg cccttcgcct tcatgtcctt gatgatgtct ttgtcggcgt ctttgacgaa
     4021 ccggcctttc cagggcgtga cttcatcggt gaacttgccg tcgaggttga ccggctgcat
     4081 caccggcaga tcgttggcct gaccgacgcg gtagtcgtct tcaccaaagg ccggcgcgat
     4141 atggacaata ccggtcccgt cttccgttga aacgaagtca gcgccaatga catagtgggc
     4201 acgtttcttc ggcgccacaa acgtgtacat ctgctcatat tcaacgccca gcagttcggc
     4261 gcccttcacc gtgcccacga tctcgaagtc tttctgcagc accggttcga ctcgcgcggc
     4321 tgccatgatc agcgtttgcc cctgatgctt caccttggcg tagtcgatat cgcgaccgac
     4381 ggcgagcgct acgttcgaca gcagggtcca gggggtggtc gtccagacca gaaagtaggt
     4441 gtcggcctga ttcttgatct tgaagcgcac atagaccgaa gggtcttcgg cttcgtcgta
     4501 gccctgggcc acttcgtgcg acgacagagc cgtgccgcat cgcgggcagt acggaaccac
     4561 cttgtggccc tcatacagca tgcctttctt ccagagctgg cccagcaccc accagccggt
     4621 ctcgatgtac tgatcggtgc aggtgatata cgggtcatcc atgtcgaccc agaacccgcc
     4681 gcgctcgcac atgcggcgcc actcggcttc gtacttgaac acgctctcgc gacacttctt
     4741 gatgaagttt tcgatgccga acttctcgat atcctgcttg caactgatgc ccagctgttt
     4801 ttcgacttcg agttcgaccg gcaagccgtg cgtgtcccag ccggccttgc ggccaacccg
     4861 gcaaccgcac atggttttgt accggggaac aaggtctttc atggccttgg tcagcacatg
     4921 tccggggtgc ggagttccgt tcgcagtcgg cggcccttca aaaaagacga agtcgggaca
     4981 gccgtcgcga ttcttgatcg tctgtcgaaa gatgtcttcc tttcgccaga attcgagaat
     5041 ttcttcttca cgtttgggcg agatcgaggt cgaggttttc tggaacaaca taagtgatga
     5101 ggtccttagc gaaggaatga aagggttgac atgagttatg aacgcggacg atcgatcggc
     5161 gatgccagca gggagtgcat gccggatcga ctcaagagaa cgatttgggc aatattgtcg
     5221 aggctgctgg tccaatgaag gcccgattca agatcagtgc cgatggcaaa cgtgccgtga
     5281 ccgcgtacca ccacgatcgg cgacgttttc agcagtgggg gaagcccgcg agcgacttcc
     5341 tcggaaccaa cggggttgtc gacagtcagg atgggaatgc cgttcgggaa atagtaccgg
     5401 ccctcgccat cgagaggcat gatgcgatca acgagaagag acagggcaat gacatgcggc
     5461 gggtgcgcat gcaccaccgc ctgggccccg gtcgcacgat agatcgcgcg atgcaccgga
     5521 atctcgcgag aggcccgtgg cgtctcgaga tcgtcgccgt cgatcagcgt ttcgacgaca
     5581 tcgttgtgcc cgagtcgatg cagcatcgaa ccggtacggg taatcgccag cattcggttc
     5641 agacgcagac tgaggttccc cgaatgggtg ttgttcagac gggcggcgaa gagatcggcc
     5701 cccacctgtt gaaagtcacg caggacgttc atggccgttt gactccatgc agatgatcga
     5761 gaccggctcc gtgcagcgtt tcgtgaatcg attgagcata cggggccttc aggaccccga
     5821 ggttggtgaa gatcgcggtg acgagatccg ggggagtcac atcgaatgcc gggttgtaga
     5881 ctggcattcc ggtcggagcc gtttgtattc cgccccattt tgccacctca tcggcacttc
     5941 gctgttcaat gacaatttca cgtccgtggc gcacgccgag atcgaaggtc gacaccgggg
     6001 ccgcgacata gaacggcacc tgatgatagt gcgccagcac cgccaggcca tacgtaccga
     6061 tcttgttggc aacccagcct tcggccgtga tgcggtcagc gccgacgacg attccctgaa
     6121 tttttccggc ggccatcagt gaggctgcca tgttgtcgca gatcagggtg gcgtcgatac
     6181 cttcttccag gagttcccag gcggtcaggc gagccccctg cagcaacggg cgagtttcat
     6241 cgacaaacac cgagaatcgc ttgttctgtt ctttcgcggc gcggaacact cccagcgcgg
     6301 ttccatagcc tccagtggcc agggctccgg cattgcagtg ggtcagccag gtgtctccgt
     6361 tgagcagata tgcggcgccg tagaaaccga tggcgcggca catgtctatg tcttcctgca
     6421 tgatcagtgt tgcctgccgc aacgcctcat cgcgagccgc tttgagacgc tgcgccgcgg
     6481 gagcggcggc gatctgctgc tgaatggctt tcgaggcgcg attgagcgcc caaaacagat
     6541 tgacggccgt ggggcgggtt tcggccaacc gagtcgccac ggcctcgaga tgcgccgcca
     6601 ctccatcagc cgagccggag tattcgcgca acccgatgta caagccaaaa gccgcggcaa
     6661 ctccgatggc cggagccccg cgcacgcgta gagcgcgaat ggcttcacac atctgatcga
     6721 ctgaggcgca gggcatcatc acccgttcgc acggaagacg cgtctgatcg agcagtgaaa
     6781 cggtgtcggt ttctggttga tactgaacgg tcgggatcat gggcaactct ttctgcatgc
     6841 ggggtttcct gatgaaacga gtcaccccga cggtgccggc gcatcataac agagacgggg
     6901 aatgagggtc aagtgttctc cttcctccag agagagaaga aaagacttgc aaatccgtac
     6961 gaggtgcggg aaaatagttg cggctctctt ttgctcttca cgatggttcg tgtaaagtga
     7021 gccaaaagta ctatctcctg gaggaactgt tcatgtccgt gaaactgttt gttggtaatc
     7081 tcccgttttc caccgatgac aaccgcctga aagaagaatt cacgaagttc ggcgccgttg
     7141 agtccgctcg cgtcgtcacc gacaagcaca ccggccgctc acgcggatac gggttcgtcg
     7201 aattttccga tcagacagcg gccgacgccg ccgtaacggg tatgaacggg cagaatatgg
     7261 atggccgccc cctcaccgtg agcgtggcca aatcccaggg tacgggcccg aacggccccc
     7321 gccgcgaacg ctcacaggac atgtaatccg gcgctcggcc ttcacggccg gcacccatca
     7381 cttccgtgat tactttccct caaccgcccg ttcgtcgggt ggttgagggt gtttctgttt
     7441 ctcacattct gactcatgac ggctgacctt gacctgcgct ttctcacgga cttcctgaac
     7501 gcagaggtgg atcgccgcga aggttccctg atcaagcggg tattgcaggc ctccgtcgac
     7561 catggcatgt acggcatgaa tctgctgcgc accctgtaca ag
//
LOCUS       DAORHD010000045         7594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9320_length_7594_cov_3.643985, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000045 DAORHD010000000
VERSION     DAORHD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9320_length_7594_cov_3.643985"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="ssrA"
                     /locus_tag="PKO06_01795"
     tmRNA           complement(<1..310)
                     /gene="ssrA"
                     /locus_tag="PKO06_01795"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            707..1618
                     /locus_tag="PKO06_01800"
     CDS             707..1618
                     /locus_tag="PKO06_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68401.1"
                     /translation="MSQSVMPEPISRFFKAAQPILKAEKGREILIEDRSDGYGNGYSP
                     TPIEVEADAAIVQVREWITQHPEQLSGDHLTSILQAVTFCSRAVDEYRARYEGAAGDY
                     DDFYSPLAYSLQRLFESLRTVLTRLDNEGVFPLTQVPIELLRTQVRVCEGGVFTQELY
                     KSLLSDAMQTGRQEPFLEIVRQQAEEVRALVLQMLAATPDRKVWIMRDLVHRFFHTQA
                     DVADDVADGFLVSLRDGMANVREEAERCLGRCGFRVFPFVRDRFVSASGNERLLLLRV
                     LKELQRNAYSAEKPVVTEFLNHLPPER"
     gene            1783..2919
                     /locus_tag="PKO06_01805"
     CDS             1783..2919
                     /locus_tag="PKO06_01805"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNV68402.1"
                     /translation="MKCRQWDLRPPFTAQAIPAAERETFDLILAFGPTDEFARPDFHP
                     QLRKAFPKAILAGCSTCGEIAQSGASEDHLIITGIRCEHGRLRSAVASVGEGDDTSAG
                     RTLAQALIAPDLRLVLVFTEGLHTHAQALVDGLLSVLPPQVLISGGLAGDGYRFQKTV
                     VLTPDSVSGTQAVAVGFYGDRLQVRVASATGWEPFGRARRVTRAQGSKVFELDGKRAL
                     EIYAGYLGPEAANLPAAGLVYPVAVETVAGSPGLIRSLSAVNHDDGSLTFFGDIPEGS
                     IVRLMNASYKELIAGASQAITHALHGAESPDQFTLGLLFSCAGRKAVLRAHTDLEIAA
                     VSQHLPDSICLTGFHTYGEIGYAEATQRCEHHNQTMTVTLLSEN"
     gene            2921..4735
                     /locus_tag="PKO06_01810"
     CDS             2921..4735
                     /locus_tag="PKO06_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV68403.1"
                     /translation="MHELLLLQLKKVLNLPATQLREIRENPHSLNALLQNTPPHLMRE
                     VLREISSAYEEHELQNQRLNRALRISAREADELATELRRQEEQKYRLLFEHMALGFLH
                     LEILFDASGEPVDARCLEANPAFLRMFSLTEQQVQGKHLLQLRPNFNRDLLRAMGEVA
                     NTGMPSRFEREDPITHRVFEVCLYRPTDKHLAALLNDVTERRREAEEKQRLELQMQQS
                     QKLESLGLMAGGIAHDFNNLLTVIGGNIDICRHQGPLPRDVVENLDDAWAALKRASDL
                     CRQLLAYSGKAPLQLSNFDLSDLLRRHTSLLEVSVPRKIAIEYQLAEHLPPIEGDPTQ
                     IEQVVMNLMINAAEAIGNASGTITLSTSSRECDAAFLASAWLKEPVAPGRYVCLKVED
                     SGCGMDAATLERIFDPFYSTKHTGRGLGLAALLGIVRRHHGTLHVTSVVGKGTSFELF
                     FPAAEKQIRDVKVHRTEESTGDERFVGTVLLVDDEPSIRDIGAQLLELLGVHVMTAAD
                     GEEALHVFRTCRQMAVNHDRQIRCLILDLTMPRMDGLEAMIELRRLDPELPILLASGY
                     TEEEITARLSEVRPNGFIQKPYSLETLRSKLAPYLSKT"
     gene            4770..5864
                     /locus_tag="PKO06_01815"
     CDS             4770..5864
                     /locus_tag="PKO06_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68404.1"
                     /translation="MTFLGQTPRQWFEFLLLFGIVLITLIWSIPQGGGAQVSGLLLLA
                     AVLSYGLFFSPHVISQPAAHSSRTEAFTMVAWSMALVWGVMPLGPECKRIAQGLLWAT
                     AAYILFVSPWRHGDTLEDRGLGTLPGFFTYLKQGEYKWLARTVFMGGNLLLIVGCFAA
                     TNVLQDVVAGALRRMGGLRIPRELPAWAWLAISLGLYNFLICFLMRWDNLSRAGPMIL
                     TYLASGILFVVLFGYWYIYLKHSGWVELVPERGFKRISSYILWGTLQELLFLSYFNTR
                     IRKGVSSPLLAAVLTATIFSVFHLRAYTLMIICYLIGIVWALMFQAAPNLMLMGLAHG
                     IGGGFGSAFAVQGMTMFKINARVGPFHPPG"
     gene            5928..7145
                     /locus_tag="PKO06_01820"
     CDS             5928..7145
                     /locus_tag="PKO06_01820"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HNV68405.1"
                     /translation="MTKLRWHFLALLLLGTWSATVCCANRPADDQAEPGRALPAPHLN
                     RLFQRTSGWTGADGASSVPLTDTLTLWLFGDTFIGRVYDGARAKTTLINNSVALQVLR
                     RDRGGEIVSARPRFYWKTARDQSQHAKLWQESRFERRANGDKPTSFFLPPDRKGWFWP
                     LSGVLADNALFVVLLQLDKTDETSVFGFRTIGLWLARVSNPLSSPSDWVVSYRRVPWC
                     LTGTERQVTFGSAIMKDGDAVYVYGIDEDLRPGKAQKSLIVARAPMAGFDRFSAWRFW
                     NGTAWGANFTRSAAIAEGLVDEFSVSRLPGTDRLVLVQTEPGLSPRIVARMAGAPQGP
                     WSTPRPVYTVPEASGSKRLFAYAAKAHPELATQPHALVLTYVVNSFDFFDLIKQANLY
                     WPTFLQLRIDSRS"
     gene            7142..>7594
                     /locus_tag="PKO06_01825"
     CDS             7142..>7594
                     /locus_tag="PKO06_01825"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV68406.1"
                     /translation="MNRNKGAQAQAALRLMRVSGVGAAMFRRLVEHYGSPGAALQAVE
                     ALRAREDGFPVPRTTVKRQTPTLLTRVLAWIEAPPARHHILWLGHSRYPLHLGQITEP
                     PPVLFCHGRLATLRQPTLAIVGAREASETGLKLTREIAAWAASQQICVV"
BASE COUNT         1434 a         2361 c         2241 g         1558 t
ORIGIN      
        1 cgtctaccat catgtcccga gtttaattgt ttaaggcttt cgtaaccccc aggcaggtct
       61 ctactcgccc gatccgtctg gaaagtctcg gcgcttcagg ccagtggccc cctgaaacac
      121 ccagccagtt taagtggcag ctctccggta tcttactggc agaatctacc gggaaccgtc
      181 gcggcgctta ggctgcgagt gctaagtttt cgttagcagt tattgtttgg tcgaattttt
      241 acgaggccgt tcgaccaacc tcggatggca ggagattgct cactctcccc gtcgaaacca
      301 ggtcgccccc agagttttca aagaacgccg gcaccggcac gcagcccgct accataccta
      361 agtatatcac aaccgctcag ttcaaatatg cgtgggcgag cgaaaaaaca caagcatggg
      421 cgcaatttcg ccctcttcga cgtgtggcat tcgaagccgg ttacccgtat catgaaatct
      481 gtgatccatc gatccgggaa tcaccaggaa tgggagtctc cgcttgcgaa tctctacggt
      541 tctcctgctg acgctgagct tctggctcac ccctgtattc cacaccgccc aagccgcaag
      601 ccggtcaccc gcccttccag aaaatcgagc ccggctcggt gaatcccgcg cctctcacac
      661 accccagggt ctcgtaccca acccatcacc tgcgaaggac aaatccatga gccaatctgt
      721 gatgcccgag cccatttcgc ggtttttcaa ggccgcgcaa ccgattctga aagctgagaa
      781 ggggcgtgag atcctgatcg aagaccggtc tgacgggtac ggaaacgggt attcgcccac
      841 tccgatcgag gtcgaggccg acgcggcgat tgtccaggtt cgcgagtgga tcacacagca
      901 cccggagcaa ctttcaggtg atcatctcac aagcattctg caggccgtca cgttctgttc
      961 acgggcagtc gacgagtatc gggcccgata cgagggtgcg gccggcgact acgacgactt
     1021 ctactccccg ctggcctatt ctctgcaacg cctgttcgag agtttgcgca ccgttctcac
     1081 gcgtctggac aacgagggag tctttccgct gacgcaggtg ccgatcgaac tgctgcgcac
     1141 tcaggtcaga gtgtgcgagg gcggcgtctt cacgcaggag ttgtacaaat cgcttctttc
     1201 cgacgccatg cagaccggcc gtcaggaacc gtttctcgag attgttcgcc agcaggcgga
     1261 agaggtgcga gccctcgtgc ttcagatgct cgcggccact ccggatcgaa aagtctggat
     1321 catgcgcgac ctcgtgcatc gcttctttca cacgcaggcc gacgtggccg acgacgtggc
     1381 cgacggtttt ctggtttctc tgcgcgacgg tatggccaac gtgcgcgagg aagcagagcg
     1441 ctgcctgggt cgttgcggtt ttcgcgtctt tccgttcgtg cgtgaccgct tcgtgagcgc
     1501 atcgggaaac gagcgcctgc tcttgctgcg ggttttgaaa gagctgcaaa ggaacgccta
     1561 ttcagccgaa aaacccgtgg tcaccgagtt tctgaatcat ctgccacccg agcggtgacc
     1621 agcgttggca ggtcgagaac gcgttgaacc cgatcacgag agatacgcgt cgtcgagtcg
     1681 gcatcggctc gtccttcgtg gtatactgcg gtcatacctg aaaaacagtg aaagtgagag
     1741 gccctgctcc cgatccgggg caggcagccg aagtatagag acatgaagtg ccgacaatgg
     1801 gatctgcgac ctccgtttac cgctcaggcg attcctgctg cggagagaga gactttcgac
     1861 ctgattctcg ctttcggacc gaccgacgaa ttcgcgcgcc ctgacttcca cccccagttg
     1921 cgcaaagctt ttccgaaagc cattctcgcc gggtgttcca cctgcggaga aatcgcccag
     1981 agcggggcgt ccgaagatca cctgatcatc accggaatcc gctgtgaaca tgggcggttg
     2041 cgatccgcgg tcgcgtcggt tggcgaaggc gacgacacga gcgcaggccg cacactggct
     2101 caggcactca tcgcccccga tcttcgactc gtacttgtgt tcaccgaggg cttgcacact
     2161 catgctcaag cgctggtcga tggtctgctc tcggtgctgc caccgcaggt tctgatcagc
     2221 ggtggactcg caggtgacgg gtatcggttt cagaagaccg tcgtcctcac ccccgacagc
     2281 gtttcgggaa cccaggccgt ggcggtcggg ttctacggtg accggttgca ggtacgggtc
     2341 gcctcggcaa ccggctggga acccttcggc cgcgcccgcc gagtcacccg cgcacagggg
     2401 tcgaaggttt tcgaactcga tggcaagcgc gccctcgaaa tctatgccgg ctatctgggc
     2461 cccgaagccg ccaatctccc ggccgccggt ctggtctatc cggtcgcggt cgagaccgtg
     2521 gcaggttcac ccggcctgat tcgatcgttg tcagccgtga atcatgatga cggttccctg
     2581 accttcttcg gcgacattcc cgaaggaagt atcgtgaggc tgatgaatgc ttcctataaa
     2641 gagttgattg cgggcgcttc gcaggcaatc acccacgcgc tgcacggcgc ggaatcaccc
     2701 gaccagttca ccctgggtct gctgttcagc tgtgccgggc gcaaagcggt gcttcgggcg
     2761 cacaccgatc tcgagattgc cgcggtgtcg cagcatcttc ccgattcgat ctgcctgacc
     2821 gggtttcata cctatggcga gatcggatac gcggaggcga cgcaacgttg cgagcatcac
     2881 aaccagacca tgaccgtgac tctgctgagt gagaactgac atgcacgaac tgctgttgct
     2941 gcaattgaag aaagtcctca accttccggc gacccagctg cgagaaattc gcgagaaccc
     3001 gcacagcctg aatgcgctgc tgcagaacac tccgccgcat ctgatgcgtg aggtgctgcg
     3061 cgagatcagc tcggcgtatg aagaacacga actgcagaat cagcgattga accgggctct
     3121 gcggatcagc gcccgcgaag cggacgaact ggccacggaa ctgcgccggc aggaagagca
     3181 aaagtaccgg ttgctgttcg agcacatggc gctcgggttc ctgcatcttg agatcctgtt
     3241 cgatgcaagc ggtgagccgg ttgatgcccg gtgcctcgaa gcgaaccccg cgtttctgcg
     3301 catgttcagt ctgaccgagc aacaagtgca ggggaaacac ctgctgcagc tgcgcccgaa
     3361 ctttaatcgt gatctgctgc gagcgatggg tgaggtcgcg aacaccggca tgccaagcag
     3421 atttgagcgc gaagacccga tcacccaccg cgtgttcgaa gtgtgcctct atcgcccgac
     3481 cgacaagcac ctggctgccc tgttgaatga tgtcaccgag cgccgtcggg aggctgagga
     3541 aaagcagcgt ctcgagctgc agatgcaaca gtcgcagaag ctcgagagtc tcgggttgat
     3601 ggccggcggc atcgcgcatg atttcaacaa tctgctgacc gtcatcggcg gcaatatcga
     3661 catctgccga caccaggggc ccctgccccg cgatgtcgtc gagaatctcg acgacgcctg
     3721 ggctgccctg aagcgcgcct cggatctgtg ccgtcagctg ctggcgtatt cgggaaaggc
     3781 cccgttgcag ctctcgaact tcgatctcag cgatctgctg cgccggcaca cctcgctcct
     3841 cgaggtttcg gtgccacgca aaatcgccat tgagtaccag ctggcagaac atcttccacc
     3901 gatcgaaggc gatccgacgc agatcgaaca ggtcgtgatg aacctgatga tcaacgccgc
     3961 ggaagccatc ggaaacgcct caggcaccat cactctttcg accagcagtc gggaatgtga
     4021 cgccgcgttt ctcgcgagcg cctggctgaa agagccggta gcgcccggcc gctatgtctg
     4081 cctgaaagtt gaagactcgg ggtgcggaat ggatgccgcc acgcttgaac gcattttcga
     4141 cccgttctat tcgaccaagc acaccggccg tggactcggc ctggcagcac tcctcggcat
     4201 cgtacgccga catcacggca cccttcacgt caccagtgtc gttggcaaag gtacgagttt
     4261 tgagttgttc tttccggcag ctgaaaagca gattcgcgac gtgaaagttc atcgaaccga
     4321 agaatcaacg ggtgacgagc ggtttgtcgg cacggttctg ctcgtcgatg acgagcccag
     4381 tattcgcgat atcggggccc aactgctcga gctgctgggc gtgcacgtga tgaccgccgc
     4441 cgatggtgaa gaagctctac acgtttttcg tacctgccgc cagatggcgg tgaaccacga
     4501 tcggcagatc cgctgcctga tcctggatct caccatgccc cgcatggacg gactggaagc
     4561 gatgatcgag ctgcggcgtc tcgatccgga actgccgatt ctgctggcca gcggctacac
     4621 cgaagaagag attaccgcgc gattgagcga ggttcgcccg aacgggttca ttcagaagcc
     4681 ctattcactc gaaactctca gaagcaagct ggcgccgtat ctctcgaaaa cgtaagacgc
     4741 tgttgcgacg aattggcgcc tgatcaccca tgacctttct cggacaaacc ccgcgtcagt
     4801 ggttcgaatt tctgctgctg ttcggcatcg tgctcatcac cctgatctgg tcaatcccgc
     4861 agggtggcgg agcacaggtt tccggacttc tgctgctggc cgcggttctc agctacggtc
     4921 tcttcttctc gccacacgtg atatcacagc cggcagccca cagcagccgc accgaagcct
     4981 ttacgatggt cgcgtggtcg atggcgcttg tctggggagt catgccgctg gggccggagt
     5041 gcaagcgcat cgctcaggga ttgctctggg caaccgccgc ttacattctg ttcgtttctc
     5101 cgtggcgaca cggtgacacg ctggaagacc gaggtctcgg aacgctgccc ggctttttca
     5161 cctatctgaa gcagggagag tacaagtggc tggcccggac ggtgttcatg ggcggcaatc
     5221 tgctgctgat cgtcggctgt ttcgccgcca cgaacgtgct tcaggatgtc gtcgccggtg
     5281 ccctgaggcg catgggcggt ctgcgtatcc cgcgcgaact gccagcctgg gcgtggctcg
     5341 cgatctcgct ggggttgtac aactttctca tctgctttct gatgcgctgg gacaaccttt
     5401 cacgagccgg accgatgatt ctgacgtatc tggcctcggg cattctgttc gtcgtgcttt
     5461 tcggatactg gtatatttac ctcaagcaca gcggctgggt tgaactggtt ccagagcgag
     5521 gtttcaagag aatcagttcg tacattctct ggggaacgct gcaggaactg ctgtttctga
     5581 gttacttcaa cacccgcatt cgcaagggag tcagcagtcc actgctggcc gcggtgctga
     5641 cggcgacgat cttttccgtc tttcacctgc gcgcgtacac cctgatgatc atctgttacc
     5701 tcatcggcat tgtctgggcc ctgatgtttc aggccgcgcc gaacctgatg ctcatgggtc
     5761 tggcccacgg catcggcggt gggttcggtt cggctttcgc ggtgcaggga atgacgatgt
     5821 tcaagatcaa tgcccgtgtc ggcccgttcc atcctcccgg ttgacgcgtt gccgccgacc
     5881 tgggtgcgtg gtagcgtagg aacaacccaa cgacgaaaag gtgaatcatg accaagcttc
     5941 gatggcactt tctggctctg ctcctgctcg gcacctggag tgccacggtc tgctgcgcga
     6001 accggcccgc tgacgatcaa gccgaaccgg ggcgggcact tcctgcaccg cacctgaatc
     6061 gtctgttcca gcgcacctca ggctggaccg gcgctgacgg cgcatcgtcc gtaccgctga
     6121 cggacaccct gacgctctgg ctgttcggcg acacgttcat cggccgggtc tatgacggcg
     6181 cacgcgccaa gaccaccctg atcaacaact cggtcgccct gcaggttctg cgccgagatc
     6241 gcggggggga aatcgtttcg gccaggccgc gcttctactg gaagacggcg cgcgatcagt
     6301 ctcagcacgc caagctgtgg caggaaagcc gattcgagcg acgagcaaac ggcgacaagc
     6361 cgacctcgtt ctttctgccc cccgaccgca aggggtggtt ctggccactc agcggagtgc
     6421 tggccgacaa cgccctgttc gtcgtgctgc tgcagctcga caagactgat gagacgtcgg
     6481 tcttcgggtt tcgtacgatc ggcctgtggc tcgcccgcgt atcgaacccg ctcagttcgc
     6541 cttccgactg ggtggtttcg tatcgccggg ttccctggtg cctgactggt acggaacggc
     6601 aagtgacctt cggttcggca atcatgaaag acggcgacgc cgtgtatgtg tatggcatcg
     6661 atgaagatct gcgccccggg aaggcgcaga aaagcctgat cgtcgctcga gcgcccatgg
     6721 ccggcttcga ccggttttcc gcctggcgat tctggaacgg cacggcatgg ggagccaatt
     6781 tcacccgatc ggcggccatt gcggaaggcc ttgtcgacga gttctcggtc agtcgactgc
     6841 ctggcaccga ccgtctggtg ctggtacaga cggaaccggg gctgtctccc cgcattgtgg
     6901 cgcgtatggc cggagctcct caggggccct ggtcgacccc tcgccccgtc tacaccgtcc
     6961 ccgaggcttc gggcagcaaa cgtctattcg cctatgccgc gaaagcccac ccagagctgg
     7021 ccactcagcc tcatgctctg gtactcacct acgttgtcaa ctcgttcgac ttcttcgatc
     7081 tgatcaagca ggcgaacctg tactggccga cttttcttca actgcgcatc gacagccgct
     7141 catgaacagg aacaagggcg cacaggcaca agcggctctc cgcctgatgc gcgtttcggg
     7201 cgtcggtgcc gccatgttcc ggcgactggt cgagcactat ggctcgcccg gagcggcact
     7261 gcaggcagtc gaagcgctgc gcgcccgcga ggacggattt ccggttccgc gaaccacggt
     7321 gaagcgacag actccgacgc tgctgaccag ggttctggcc tggatcgaag ccccaccagc
     7381 ccgtcaccac attctctggc tcggacattc gcgctatccc ctgcatctcg ggcagatcac
     7441 cgagcccccg cccgtgctgt tctgtcacgg tcgtctggcg accctgcggc agccgacgct
     7501 cgccatcgtc ggcgctcggg aagccagtga gaccggcctg aaactcaccc gcgagatcgc
     7561 cgcctgggcc gcgtctcagc agatctgcgt ggtc
//
LOCUS       DAORHD010000046         7590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9329_length_7590_cov_3.076178, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000046 DAORHD010000000
VERSION     DAORHD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7590
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9329_length_7590_cov_3.076178"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKO06_01830"
     CDS             complement(<1..1197)
                     /locus_tag="PKO06_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV68407.1"
                     /translation="MKQFCRTFLALLLVLHAGAGFSVEFSTFQARGYEGRGPGEFIGP
                     EDLILGPSGEIVVADSRNNRVQVLSSAGIFQRMIWLRQETPGVASYISDLEKSLASGT
                     AKSVAGTKKDDAEEQVGKKSDRAERIRLPNGTSISMDKPSGLALDSQGRLFVACPGSN
                     QILIFRLTDGVFLGMFSRPGRMQGTLDGPIDIDVRQDGLLAVAESTAKRVQIFNPDGS
                     FVRELLYKEETPKRGLQSLVPRGVFWMPGGELLVSYPTFNQVVAWDMKGNIVWRYGVK
                     GSGKGELSEPSFFCHGPEGHVFVSDSGNHRIVEITPMGLFVKNFAMGRGSSPGRLLRP
                     GGIAFTSADSLFVADTGNNRVQIFTPSRAALILKDAKELTMKDRWDEALPRIEQVLTL
                     EPNDAEA"
     gene            complement(1235..2491)
                     /locus_tag="PKO06_01835"
     CDS             complement(1235..2491)
                     /locus_tag="PKO06_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNV68408.1"
                     /translation="MQRLLATFGFVVGISVILVAITWGIFKLQGPGPVPQPSHPTTVV
                     TSPATEAVMPTPAQPAPQEVQVVFQKDGKTYYSGKAPLLTVSGQAGAPTAIDDQQVVE
                     LVKQIPELPVPQGMVSARIETDKNGQPRRFLRGPVAPAIDRGETQRQIREALSKDPSK
                     TAIAVPIALTQAEAAGGFEALREKQGFKTCLASFVTVHVDHMDDTGRNENLRLAAERI
                     DGVVVAPGEEFNFDKVVKERSRKNGFQLAGVISNGKVIPGLGGGICQVSTTLYRVALQ
                     SNLTITERHNHSIYDGIEYADRGLDAAVAWGSKNFRFRNTLSVPILLSVMSGPGRVQA
                     AIYAPEKPFDKVDVVTRNERKLPLEKKTVINAKLKAGQVKVVQPGVIGYTIESYRIVT
                     VNGISREERLSKDKYLTFPRIEEVNN"
     gene            complement(2520..3533)
                     /locus_tag="PKO06_01840"
     CDS             complement(2520..3533)
                     /locus_tag="PKO06_01840"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNV68409.1"
                     /translation="MNTFAVIGAGSWGTTLAHLIHRNVRQVRVWAFEPEVIEGFRQSR
                     RNLFFATELLFEPHVTVLNDLRAVCEEAEAIVFAVPSKHLRTLARAAAPFISETACIL
                     NAAKGLEPGSGKRLSQVLAEELRPTASPDTDRIAVLSGPNLAHEVAAGKVGASVIASP
                     DESVSTPLQHAVSAKSFRVYRHTDRTGVELGGTLKNIFAIGAGIVEALELGDNARAAY
                     LTRALHELVRLGVRMGGRQSTFYGLAGLGDLMATATSPLSRNHQFGKALALGTTEAFQ
                     AGHQRMVVEGVETARMAREWSKKLTIPMPITEEICRVLFEGKSPREAARDLMSRSLKD
                     EEE"
     gene            complement(3553..4176)
                     /gene="plsY"
                     /locus_tag="PKO06_01845"
     CDS             complement(3553..4176)
                     /gene="plsY"
                     /locus_tag="PKO06_01845"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNV68410.1"
                     /translation="MSDVSFALLAQVALGGYLLGSIPSAYWFCLAVYRIDIFQFGSKN
                     MGATNVHRVLGQGPFSMVLVLDILKGFFAVLFGAWMAAAPEQVMLLKIVAGGTAVIGH
                     TLSFWVRFRGGKGVATGLGVFLGLAPVSSILTMFVFLVVLIPSGMVSLGSIAASASLP
                     FLIYRQGEGGDWNTALVAFATVVALVIIVKHRANISRIAAGRENKLE"
     gene            complement(4187..5527)
                     /gene="der"
                     /locus_tag="PKO06_01850"
     CDS             complement(4187..5527)
                     /gene="der"
                     /locus_tag="PKO06_01850"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNV68411.1"
                     /translation="MRQLPIVAIVGRPNVGKSALLNRIVGRRHAIVEATPGVTRDRNY
                     APATWNGKSFHVVDTGGLDPDDKTPIQESINTQVEFALHEAEAIVFLCDFSSGLMPHD
                     RQIFNQLRRKVGVKPLLVAVNKVDNPRSDDEIYEFWELGVDKLYPISAVHGHGVADLL
                     DDLVPRFPEQSDRDDEAETDHMIALVGKPNVGKSSLLNRILGQDRSIVDDTPGTTRDT
                     IHVTVRHGEKAYHFIDTAGLRRPARRKDSIETFSVIRTLQTIERSDIAVLLLDATEGE
                     ISEQDKRIAGQIIEADSACIIAWNKWDIATKDQHTWETLYRKTREEFPLLQFAPVFSL
                     SAKSGLRVDRLFELVDQVREAGAYRIPQSRLNDILFEALTIQPPPSCFGRPLVIEDLH
                     QCPGPGITFRVRCSEPKGLHFSYQRYLLNQIKQEYQFFGWPIKMQVARRSNRKH"
     gene            5843..6565
                     /locus_tag="PKO06_01855"
     CDS             5843..6565
                     /locus_tag="PKO06_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68412.1"
                     /translation="MIEYSPLLPIQELDLAIDELDRKVAQLQEKFEKLAVEIKKEEEA
                     LAAKESLFKKITLRRSQAELENDHLADRIRSAELKLQTPGVPPVAYQSLEKEIAENRE
                     KASVLETRILEDMEKSEQLAREIEKARKVATGRQQQLDEFKVKIKTQKQGLLAEKETQ
                     VTKRRQATLGLAANLLETYEDVRQRTKGRVIWEAETPGCPSCGISLPAHVVRQLSQNL
                     QKAGPCPACGAYMRWTGLVDAA"
     gene            6567..7508
                     /locus_tag="PKO06_01860"
     CDS             6567..7508
                     /locus_tag="PKO06_01860"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV68413.1"
                     /translation="MRRRCVLVLMLLLWSMTAVAQTASDAASTTLSASSPELLQVSFA
                     RYVSDDAGLIREPVLSQIIACCSEIEQNLKIRIMIKTEFLADMESYAERVDSYFSEWI
                     RSGVAEKRGILLYAGLPRDSLRGKFNLRVGIGLKYLITKEMGEKIVNQVILPNNLDNK
                     DGQGFLEGVLTVKRTLLDEYKREQARTVPPTGTFELGSFLWRSKELFFALLVALVISY
                     FIFFVERCPRCGGHLKHEDEMLKNPGESALGLKRKTYSCDRCGFSRRKKELVYPSGAQ
                     GWRLWLAGTRRHGAPVAHESAPPAESAAEKTGHQPPE"
BASE COUNT         1620 a         2096 c         2304 g         1570 t
ORIGIN      
        1 tgcttcggcg tcattgggtt cgagcgtcag tacttgttca atgcgaggga gagcttcgtc
       61 ccaacgatct ttcatggtga gctcttttgc gtccttgaga atcagtgcgg cgcggctggg
      121 ggtgaagatc tgtacccggt tgttgccggt gtcggcgaca aacagagagt cggcactggt
      181 gaaggcaatg ccgcccggac gcaatagtcg cccgggcgaa gaaccgcgcc ccatggcgaa
      241 gtttttcacg aataacccca tgggggtgat ttccacgatg cgatgattgc cactgtcact
      301 gacaaataca tgcccttcgg gaccatggca gaagaatgag ggttcggaca actctccctt
      361 gccgcttcct ttcaccccat accgccagac aatgttgcct ttcatgtccc aggccaccac
      421 ctgattgaag gtgggataac tcacgagcag ttctccgccg ggcatccaga atacaccgcg
      481 tggaactaac gattggaggc cacgtttcgg agtttcttcc ttgtacaaaa gctcgcggac
      541 gaagctgccg tccggattga agatctgcac gcgcttggcc gtgctttcgg ccacggccag
      601 caggccatcc tggcgaacgt cgatgtcgat tggtccatcg agagtgccct gcatgcgccc
      661 gggccgtgaa aacatgccga gaaacacccc atcggtcaac cgaaagatca aaatctggtt
      721 cgaacccgga cacgcgacaa acagacgtcc ctggctgtca agcgccagac cactcggttt
      781 gtccatcgag atgcttgtac cgttgggaag tcgtatgcgt tcggcgcgat ccgatttctt
      841 gccgacctgt tcctctgcgt catctttttt tgtcccagca acggattttg ctgtgccgct
      901 ggccaagctc ttctccagat ccgaaatgta actggcgacg cccggcgttt cctgcctgag
      961 ccagatcatg cgctgaaaaa tgcctgcgct gctgagcacc tgaacacgat tgttgcgact
     1021 gtcggccacc acgatttcgc cgctcggacc cagaatcaaa tcttcgggcc cgatgaactc
     1081 gcccgggccg cgtccttcgt atccgcgggc ctgaaaagtg ctgaattcga cagaaaaccc
     1141 tgctccggca tgtagtacca gaagcagggc cagaaacgtg cggcagaatt gtttcatggt
     1201 gtcttccccg ccggggcgat gaaccccggg cgactcagtt gttgacttcc tcgatacgcg
     1261 ggaatgttaa atacttgtcc ttgctcagtc gttcttcacg actgatgccg ttcaccgtga
     1321 cgatacgata ggactcaatg gtgtacccga tcacgccggg ctggacaact tttacctgtc
     1381 cggccttgag tttcgcgtta atgaccgtct tcttttcgag tggcaacttg cgctcgttgc
     1441 gggtgacgac gtcgaccttg tcgaagggtt tttccggggc atagatggcg gcctgcacgc
     1501 gaccgggccc ggacatcacc gacaacagaa tgggcactga gagcgtgttg cgaaagcgga
     1561 aattcttcga cccccatgcc acggcggcat cgagaccgcg atcggcatac tcgatgccgt
     1621 cgtagatcga atgattgtgc cgttcggtga tggtgagatt gctctgcaga gcgacgcgat
     1681 acagcgtggt actcacctgg cagatgccgc cgccgagacc gggaatcact ttcccgttcg
     1741 agataacgcc ggcaagctga aacccgttct tccgactgcg ttccttgacc actttgtcga
     1801 aattgaactc ttcgcccggt gcgaccacga cgccgtcaat gcgctcagcg gccaggcgca
     1861 gattctcgtt gcgtcccgtg tcgtccatgt gatcgacatg cacggtgacg aagctggcca
     1921 ggcaggtctt gaacccttgt ttctcgcgca aggcttcgaa tccgccggcg gcttctgcct
     1981 gcgtcagagc gatggggacc gcgatggcgg tcttcgacgg gtctttactg agggcttcgc
     2041 gaatctggcg ctgcgtctcg ccgcgatcga tggccggtgc gaccgggcca cgcagaaagc
     2101 ggcgtggctg gccattcttg tcggtctcaa tgcgggcact gaccattcct tgcggaacgg
     2161 gaagctcagg aatctgtttg accagctcaa ccacctgctg atcatcgatg gcggttgggg
     2221 ccccggcttg tccgctgacc gtcagcagag gagcttttcc ggaatagtag gtttttccgt
     2281 ctttctgaaa cacgacctgc acttcttgtg gcgcaggttg agcgggggtg ggcatgacgg
     2341 cctccgtcgc gggtgaggtg acaacggttg tcgggtgcga gggttgtgga acagggccgg
     2401 gaccctggag cttgaagatc ccccaggtga tcgcgaccag aatgacgctg attccgacaa
     2461 caaatccaaa ggtagccaac aatcgctgca tgatcaaaac tccagagaaa aagtccgggt
     2521 cactcttctt cgtccttgag agagcggctc atcagatctc gggcggcctc ccggggagac
     2581 ttgccttcga agagaaccct gcatatctct tcggtaattg gcatcggaat ggttagcttt
     2641 ttgctccatt cccgcgccat acgtgccgtt tcgaccccct cgaccaccat gcgctggtga
     2701 cccgcctgaa aggcttcggt ggttccgagg gccagcgctt tgccgaattg atgattgcgc
     2761 gacaggggtg acgtggcggt cgccatgaga tcgcccaggc cggcgagccc gtagaaggtg
     2821 ctctgtcggc cgcccatgcg cacaccaagc cgcaccagtt catggagcgc gcgcgtcaga
     2881 tatgcggcgc gagcgttgtc acccagttcg agcgcttcga cgatcccggc accgatggca
     2941 aaaatgtttt tcagggttcc gccgagttca acgccggtac ggtcggtgtg gcgatagacg
     3001 cggaacgact tcgctgaaac cgcatgctga agcggggttg acacgctctc gtcgggagac
     3061 gcgatcaccg atgcaccgac tttcccggca gcgacctcgt gtgccaggtt ggggcctgat
     3121 aacaccgcga tccgatcggt gtccggggag gccgtggggc gcaattcttc tgccagaacc
     3181 tgcgacaggc gcttgcccga acctggttcg aggcccttgg cagcattgag gatacacgcg
     3241 gtctccgaga taaacggagc cgcggcgcgg gcgagagtcc gcagatgttt cgaaggaacg
     3301 gcgaacacga tcgcctcggc ttcttcacag acggcgcgaa ggtcgttcaa gacggtcaca
     3361 tgcggttcga acagcagttc ggtggcgaaa aagaggttgc gccgcgattg gcgaaaccct
     3421 tcgatgactt caggttcaaa agcccacacg cgaacctgtc tcacattccg gtgaatgagg
     3481 tgagccagag tggttcccca ggatccggcg ccgatgactg cgaaagtatt catgaatggg
     3541 gtgcctgaca aactattcga gcttgttttc gcgaccggcc gcgatgcggc tgatgttggc
     3601 ccgatgtttg acgatgatga ccagggcaac caccgtcgca aacgcaacga gggcggtgtt
     3661 ccagtcacca ccttcgccct gacggtagat cagaaagggc aacgaggccg aagccgcgat
     3721 agaaccgagc gagaccattc cgctcggaat caggaccacc agaaacacga acatggtgag
     3781 aatcgacgag accggggcga gtcccagaaa aacgccgaga ccggtggcaa cgccttttcc
     3841 gccgcgaaat cgcacccaga acgacagggt gtgaccgatg accgcggtgc cgcctgccac
     3901 gattttgaga agcatcacct gctcaggtgc cgctgccatc caggctccga acagaacggc
     3961 aaaaaagccc ttgagaatat cgagtaccag caccattgag aacgggcctt ggccgagaac
     4021 ccggtgcacg ttcgtcgcgc ccatgttttt tgagccgaac tgaaagatat cgatgcggta
     4081 gactgccagg cagaaccaat aggcggaagg aatcgacccg agcagatacc cgccgagagc
     4141 gacctgggcg agcagcgcga aagagacgtc agacatggcg aacctctcag tgtttgcggt
     4201 ttgagcgccg ggccacctgc attttgatgg gccaaccgaa gaactggtat tcctgcttga
     4261 tctggttcaa gaggtagcgt tgatacgaaa aatgcaggcc cttgggctcg gaacaacgaa
     4321 ctcggaacgt gatgcccggc cccggacatt gatgcaggtc ttcgatgacg agtggccttc
     4381 caaaacagga ggggggcggc tgaatcgtca aggcttcgaa cagaatgtca ttcaaacggg
     4441 actgcggaat tcgataggct ccggcttcac gcacctgatc gaccaactcg aacaagcggt
     4501 cgactcgcaa accacttttg gccgacagtg aaaataccgg agcaaactga agcaggggaa
     4561 actcttctcg agtcttgcga tagagcgttt cccacgtgtg ctgatctttt gtcgcaatat
     4621 cccacttgtt ccaggcgata atgcaggcgg aatcggcctc gatgatctgg ccggcaatgc
     4681 gtttgtcctg ttcggatatc tccccttcgg tggcgtcgag cagcagtacg gcgatatcgc
     4741 tgcgctcgat ggtctgcagc gtgcgtatga ccgaaaacgt ctcgatcgag tctttgcggc
     4801 gagccgggcg acgaagaccg gcggtatcga tgaaatggta ggctttttcg ccatgtcgaa
     4861 cggtcacatg gatggtgtca cgagtggttc ccggggtgtc gtcgacgatg gaccggtcct
     4921 ggccgagaat gcgattgagc agcgatgact tgccgacatt gggcttgccg acgagggcaa
     4981 tcatgtggtc ggtttcggct tcatcatcac ggtcgctttg ttcgggaaaa cgtggaacca
     5041 ggtcgtcgag aaggtcggcg actccatgac catgcaccgc cgagatcgga tacagcttgt
     5101 cgaccccgag ttcccagaac tcgtagattt cgtcatcgct tcgcggattg tcgaccttgt
     5161 tgacggcaac cagcaagggt ttgacgccga ctttgcggcg cagttgattg aagatctgtc
     5221 gatcgtgcgg catcaagcct gaggaaaagt cgcagagaaa gacgatcgct tcggcttcgt
     5281 gcagggcaaa ttcgacctgg gtgttgatcg attcctgaat gggggtcttg tcgtcgggat
     5341 cgagaccgcc ggtgtcaacg acatgaaacg atttgccgtt ccaggtggcg ggggcgtagt
     5401 tgcggtcacg ggtgaccccg ggtgtggcct caacgatcgc atggcgacgg cccacgatac
     5461 gattgagcag agctgacttg ccgacgtttg ggcggccgac gattgcgacg atgggaagtt
     5521 gacgcatgat gcctcctgaa agaaggtctc tacagtacca gaaccagagg aaaaagtcaa
     5581 agggcgcggg cgagaaaaac ctgatgtaaa gccgcttgac gtgtattgct gggcgcgcct
     5641 gccgttggta tcatgaaccg gcgagttccg gttctgcgag cagaaagggt cttgattcgc
     5701 atgaatgcag ggacgtgacg gaaatgtgag gcaggcacgg tctcgataac ccaaactggc
     5761 cgtgtccttg aaatcgatcg tttgacagtg gtggggctgt ttgatagtgt accccgcaaa
     5821 accatcgcag gaggagcgac gcgtgatcga gtattccccc ttgttgccga ttcaggagct
     5881 cgatctcgct atcgacgagc tagaccgcaa agtggcccag ctgcaggaga agtttgagaa
     5941 gctggcggtc gagatcaaga aagaggaaga agcactcgct gccaaagaga gcttgttcaa
     6001 gaaaatcacg ttgcggcgca gtcaggctga actcgagaat gaccatctcg ctgatcgtat
     6061 tcgctctgcg gaactgaaac tgcagactcc tggtgtcccg cctgttgcat accagagtct
     6121 cgagaaagag attgccgaaa accgcgagaa ggcatcggtt ctcgaaacca ggattctcga
     6181 agacatggaa aaaagcgagc agctggcacg tgaaattgag aaagcccgca aggtggcgac
     6241 ggggcgacag cagcagctcg acgagttcaa ggtcaaaatc aagacccaga aacaggggct
     6301 gctggcggaa aaagagaccc aggtcaccaa acggcgtcaa gcgactctcg gtctggcggc
     6361 gaatctgctc gaaacctatg aggacgtgcg ccagcggacc aaaggtcgcg tcatctggga
     6421 ggccgagacg cccgggtgtc cgtcgtgtgg catttcactg ccggctcacg tcgttcgcca
     6481 gctcagccag aatctgcaga aagctgggcc ttgccccgcc tgcggtgcgt atatgcgctg
     6541 gacaggcctg gtggatgctg cttgacatga gacgtcgctg cgtgctcgtg ctgatgcttc
     6601 tgctctggtc gatgacggcc gtggctcaaa cggcctctga tgcagcctcg acaacgttgt
     6661 cggcatcgtc gccagaactg ctgcaagtgt cgtttgcgcg ctatgtcagt gatgatgcgg
     6721 gtctgattcg cgagcctgtt ctgtcgcaga tcattgcttg ttgcagtgaa atcgagcaga
     6781 acctgaagat ccgcatcatg atcaagacag agtttctcgc ggatatggaa tcttacgcgg
     6841 agcgcgtcga ctcttacttt tcggaatgga ttcgcagtgg ggtggcggaa aaacgtggca
     6901 ttctgctgta tgccgggctg ccacgtgatt ctctgcgagg caagttcaac ctgcgtgtcg
     6961 gtattggcct gaaatacctg atcacgaaag aaatgggcga gaagatcgtc aatcaggtca
     7021 ttctgccgaa caatctcgac aacaaagacg ggcaggggtt cctcgagggc gtgctgaccg
     7081 tgaagcgcac gctgctcgat gagtacaaac gcgagcaggc cagaacggtg ccgccaacgg
     7141 gaactttcga gctgggaagc tttctctggc gatccaagga gttgttcttt gctttgctgg
     7201 tggccctggt gatcagctac ttcatattct tcgtcgagcg atgtccccgt tgtggcggac
     7261 atctgaagca cgaagacgag atgctgaaga atcccggcga gagtgctctg gggttgaagc
     7321 gaaaaacata ttcctgtgat cgctgcggtt tttcccgccg caagaaagaa ctggtgtatc
     7381 catcgggtgc ccagggttgg cgtctctggc ttgctggcac ccgccgccat ggcgctcctg
     7441 tcgcccacga atccgctcca ccggcggaat ctgctgccga aaagactgga caccagcccc
     7501 cggagtgagg tagactgacg tcgcaggaca gccggatggt cgcgttcggc ctggtgctgt
     7561 acctgaccgg gcctcgcatg gccgccctga
//
LOCUS       DAORHD010000047         7578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9348_length_7578_cov_3.920510, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000047 DAORHD010000000
VERSION     DAORHD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9348_length_7578_cov_3.920510"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="PKO06_01865"
     CDS             <1..1195
                     /locus_tag="PKO06_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68414.1"
                     /translation="LVPVNGQQTLALGREFFATAGSGQAVLYQPAGLSSAPTAVSAPA
                     VAPVPIVPTVGTFQPDLPVSPVPQVNPPSVAPNPVVSPAAAMPPQSALTTAGALPPSP
                     VLPSSAPMPGNPDPFFGATSVPPGLVGPLVTALAWDGRSFWAGTLNDGVWRFVDGTWT
                     HWNSAGGQLKDDQVVNLYTAHGQTYLYSWILGLVALENTAPRPVFPAESVQGLLSVAS
                     GDQSYVYLLFKEGFIRRFKAGSPLEEVGRVPEDFYRSVHSLQMLQGKPMVVVNDGVIA
                     QDQTGRWITSFFEEDVKGRKPLFSTLSADGRLIIALDDGRVFAYHQRRIQRIAALGTR
                     PRSLMSSGRDVWLLTSTSIYRLENNGFATVPLPQTGPFQQQLSLPERQIIVVLSETGV
                     RGFRF"
     gene            1346..1690
                     /locus_tag="PKO06_01870"
     CDS             1346..1690
                     /locus_tag="PKO06_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68415.1"
                     /translation="MNLYGTDEELEEFNQLIKNPQVERNLYAMALIIEEIKRRNFLKM
                     SWHCCWRCQYFATCKINWYRGERKMERRNCCTYCQNFWECHKRFNEEADVSDATTETK
                     PETPPKDPGTKP"
     gene            1632..2276
                     /locus_tag="PKO06_01875"
     CDS             1632..2276
                     /locus_tag="PKO06_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68416.1"
                     /translation="MQRQKQSLRPLPRIPAPSRESFLLTDRISLVVLIVCGFLLLPAI
                     AGADLTPDGPSGYVRVPSHTTIKYRQVEWSAHARQYKSPGAGRLRSLTDLAMGFSPIK
                     DFELGFQKTLDTNRATEAYDPDPTVNFKVRLPSMGDGYFSETAIGMILDTNPNNYHSL
                     YFTVGGFGVAWNFGGNPYVGTARYGTYDRGRHQPKNLCLIVGADLSPGVPGERG"
     gene            2310..2441
                     /locus_tag="PKO06_01880"
     CDS             2310..2441
                     /locus_tag="PKO06_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68417.1"
                     /translation="MFSLGWRLKSHRGFWVDAAASTKSTYTDFYDYQPLTIGVGAIF"
     gene            2515..4086
                     /locus_tag="PKO06_01885"
     CDS             2515..4086
                     /locus_tag="PKO06_01885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNV68418.1"
                     /translation="MRLVNASEMRAIDQFAMQKIGLTSRVLMENAGLKVLFTLERVFQ
                     GLRGKRFTVVCGKGNNGGDGLVMARHLLNNGVSVNVFVVAHEQDLTGDCAFQAEVMKN
                     CGYDCAFMTSREDLDKLRVTLEFSDVAVDALYGTGFSGNVDGYAAEVVRIFNDCRGHR
                     VAIDVPSGVCSTTGRVSDPHVNADLTITLGLPKLGLYLPPGLDAAGDVWLADIGIPPR
                     ALEVVAPRAYLVDQRLVQTLLPTRPEQSHKGTFGHVMILAGSHEYQGAGVLATYGALR
                     SGAGLISLGLPEDVANGMTCEVLPDVITRRFPAKDGGFALDAEQVRAFVGQYRAVVAG
                     PGWGRQQGRRTSLLALIQNWAGGLVLDADALNLLESMTELRGHGSDLVLTPHLGEMSR
                     LTGKSIAVLKDDLPGAARELAERAQAVVLLKSAVTVIAIPDGRIFLLSRPNSGLARGG
                     AGDLLAGLVGGFMAQGIPGWQAAIVGAFLHSEAGRIARDELGADAMTISEVASLIPRA
                     FRQLRGEVSPPTQQV"
     gene            4100..4960
                     /locus_tag="PKO06_01890"
     CDS             4100..4960
                     /locus_tag="PKO06_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV68419.1"
                     /translation="MIRLLSLGSGSRGNASLIEIGRSFYLIDAGLSFRRISQSLAQCG
                     FDLDRLAGIIVTHEHDDHVKGLQTALKKTDVPVYATPGTIDALIDDNLDNRRFVPVRS
                     GEELDLGKIRVWPFPVPHDAAEPIGLRLEACGRTIGIASDMGHVTPVILEHLTDCDLL
                     CLESNYDDDLLSVCRYPDWLKRRIRGPRGHLGNRGVRGILSRLKRQLQHLMLFHISQE
                     SNTPELVRENVEEILRLPLLQETRLIVASQDEPTPILDLLAEQGYARSAVWRQQRFTD
                     LYAHGNLHDW"
     gene            4950..5357
                     /locus_tag="PKO06_01895"
     CDS             4950..5357
                     /locus_tag="PKO06_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNV68420.1"
                     /translation="MIGKIQERPYRSLVENYLSRCHGRLSVQLLPCRDDAELMRRFGE
                     REWTVALDERGTTPDSLGFSQWLSGHFNAGRQKVSVFLGGSAGLPAEIKTKSRERLAL
                     SRLTLNHQLALLVFAEQLYRGTSILFGEPYHKA"
     gene            5354..6199
                     /locus_tag="PKO06_01900"
     CDS             5354..6199
                     /locus_tag="PKO06_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNV68421.1"
                     /translation="MIHLSPQAFMRIENFVLNFSGIVMARLQQKGIHERIQKHLERLN
                     VQNVDDYLLLLESTSSSQVRDELMSLVTVGESFFYRNPAQLQYLRQHLLPELFERKRA
                     SGSHLLRVWSAGCSTGEEPYSLAHLLSTYAGTYPEYTCSITAGDINRLNLQWAEKGIY
                     RRRSVRAQVKEIEQLLGKPLGRDLDDGRFAVDPSLRALIEFQFQNLHDLESLKTLWGS
                     DIIFCRNVLIYFEETFKERLVQTFFHLLNPGGMLFLGETESLSGIEQGFKLVSCGGAY
                     GYRKE"
     gene            6217..7281
                     /locus_tag="PKO06_01905"
     CDS             6217..7281
                     /locus_tag="PKO06_01905"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNV68422.1"
                     /translation="MEQPKKIRTLIVDDSAFMRTAIERMLKEEPSIEIVGSAANGKEA
                     VEKTFRLRPDVVTMDIEMPVMDGLHALKEIMRLCPTPVIMVSSMTQEGAKATLDALDL
                     GAFDYLGKPGSGFTTNILTLKEELLNKVRAAGDSTARVPKLRIVPLVPKRVTTTTDQM
                     TLLDWVIVVGASTGGPPAIQNILSALPAGLPAPIIIAQHMPRSFTSAYAMRLNTLCNL
                     RVKEASDGEVLQRSVAYICPGDFQTRFKRREDNRYYFSMTSNDEEKARFAPCIDNLFF
                     SAAENFGRKTLGIILTGMGEDGVRGLKNIKLVGGLTLAQDRATSVVYGMPRAALEHGA
                     VTRIIALPDIPGEIELTLKS"
     gene            7397..7543
                     /locus_tag="PKO06_01910"
     CDS             7397..7543
                     /locus_tag="PKO06_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68423.1"
                     /translation="MKNRIIPLLQDYSSSLLGFIRYLRDRVPCVPGADRGTDLDSDEE
                     DFRS"
BASE COUNT         1523 a         2077 c         2230 g         1748 t
ORIGIN      
        1 gctggtgcct gtgaatgggc agcagaccct ggcattggga cgcgagttct ttgccacggc
       61 cggatcggga caagcggtgt tataccagcc ggctggcttg agttcggctc cgacagctgt
      121 ttctgcgcct gcggttgctc cggttcccat tgttccaacc gtcggcacct ttcagcccga
      181 tcttccggta tcgcccgtac ctcaggtgaa tccaccatca gtcgcaccga atccggttgt
      241 gtctcccgcc gcggctatgc cgcctcagtc tgccttgacg accgcaggag cgcttccccc
      301 atcgccggtc ttgccttcct ccgctccgat gccggggaat cctgatccat tcttcggtgc
      361 cacgtcagtt ccccctggtc tggttggccc gctggtaacc gcgctggcct gggacggtcg
      421 ctcattctgg gctggcacat tgaatgacgg tgtgtggcga ttcgtcgacg gaacctggac
      481 tcactggaac tcggccggcg gccagctcaa ggatgaccag gtggtgaacc tgtacacggc
      541 ccatggccag acgtatctgt attcctggat ccttggcttg gtggcactcg agaatacggc
      601 tcctcgtccg gtctttccgg ccgagtcggt gcaggggctg ctgtcggtgg cttcgggcga
      661 tcaatcatat gtgtatcttc tgttcaagga aggattcatt cgacgtttca aagctggcag
      721 ccctcttgaa gaagtcggtc gggtgcctga agatttttat cgctcggtgc attcgctgca
      781 gatgctgcag ggaaaaccga tggtcgtcgt caacgatggg gttattgcgc aagatcagac
      841 cggtcgctgg atcacctcgt ttttcgagga ggatgtcaaa gggcgcaaac ccctcttttc
      901 cacactgtct gccgatggaa ggttgatcat cgccctggat gatggtcgcg tgtttgctta
      961 ccatcagcgg cgtatccaga ggatcgccgc gttgggaacc cgccctcgat cgctgatgtc
     1021 atcgggtcgt gacgtctggt tgctgacatc gaccagtatc tatcgtcttg aaaacaatgg
     1081 gtttgccacg gtaccgttgc cgcagaccgg gccgtttcaa cagcagctgt ctctgccgga
     1141 gcgacagatc atcgtcgttt tgagtgaaac cggggtcaga gggttccggt tttaagaaaa
     1201 agttcttata cttgtaccac gttattctca ggcgatgttc gaattcgtcc gatagaaggg
     1261 gtgttcttcg ggaaacgaag ggcgcaaaac tggtgtcaaa cctgtgagcg aatatctacc
     1321 ggtttttgca gaagagatct tcatcgtgaa cctgtacggg acggatgaag agctggagga
     1381 gttcaaccag ctcatcaaaa acccgcaggt agagcgcaat ctgtacgcga tggcgctcat
     1441 tatcgaggag atcaagcgcc ggaattttct gaagatgagc tggcattgct gctggaggtg
     1501 ccaatacttc gccacctgca agatcaactg gtatcgcggg gagcgaaaga tggagcgccg
     1561 taactgctgc acctactgcc agaacttctg ggaatgccac aagcgattca acgaagaagc
     1621 ggatgtatcg gatgcaacga cagaaacaaa gcctgagacc cctcccaagg atcccggcac
     1681 caagccgtga atcattcctg ctgactgaca ggatctctct cgtcgtcctg atcgtctgcg
     1741 ggttcctgtt gcttcctgcc attgccggcg ctgacctgac ccccgacggc ccttccggct
     1801 atgtgcgcgt tccatcccat accacgatca agtatcgtca ggttgaatgg tcagcacatg
     1861 cccgccagta caagtcaccc ggtgccgggc gtttacgctc actcactgat ctggccatgg
     1921 gcttctcgcc gatcaaggat tttgaacttg gctttcagaa aacgcttgat acgaatcggg
     1981 caacggaagc ctacgatcct gacccgaccg taaacttcaa ggtgcgattg cccagcatgg
     2041 gcgatggcta tttctcggaa acggccatcg gcatgattct cgataccaat ccgaataact
     2101 atcattccct ctatttcacg gtgggcgggt tcggagtggc ctggaacttt ggtgggaacc
     2161 cgtatgtggg cacagcgcgt tatggaacgt atgatcgggg tcgtcatcaa ccgaagaatt
     2221 tgtgcctgat cgtgggggcg gatctcagcc cgggggtccc cggcgagcgc ggctaacgca
     2281 gtcactactt cgccgactac aatggcgacg tgttttctct gggctggcgg ctgaagagtc
     2341 atcgcggttt ctgggtcgat gccgcggcca gcaccaagag cacctacaca gacttctacg
     2401 attatcagcc gcttaccatc ggagttggcg cgattttctg atcctctctg gctcttgtca
     2461 gaggttcacc ggattgctat agtgaacccc gtcagttgac ggcaggagga tatcatgcgt
     2521 ctggtgaatg cctcggaaat gcgggctatc gaccaatttg ccatgcagaa aatcggactg
     2581 acgagccggg ttctcatgga aaacgcgggc ctgaaggtgt tgttcacgct tgaacgggtc
     2641 tttcaggggc tgcgtggcaa gagatttacc gttgtctgtg gcaagggaaa caacggtggc
     2701 gacggtctgg tcatggctcg gcacctgctg aacaatgggg tgtcggtcaa cgtgttcgtg
     2761 gtggcccacg aacaggatct caccggagac tgcgcctttc aggcagaagt catgaagaac
     2821 tgtggctatg actgtgcctt catgacttct cgcgaagatc tcgacaaact gcgcgtgaca
     2881 cttgaattca gcgatgtggc ggttgacgcc ctgtacggca ccggcttctc cggaaacgtc
     2941 gatgggtatg cagctgaagt ggtgcgtatt ttcaacgact gccgggggca ccgggtggcc
     3001 atcgatgtcc cttcgggggt ctgctctacc accgggcggg tttctgaccc tcacgtgaat
     3061 gccgatctga ccataactct cgggctgccc aagctcgggc tgtatctgcc acccggtctc
     3121 gatgcagccg gtgatgtctg gttggccgac atcggaatac cgccgcgtgc cctcgaggtc
     3181 gtcgctcctc gtgcttatct cgtcgatcag cgtctggtac agactctgct gccgacccgc
     3241 cccgagcagt cacacaaagg cacttttggt cacgtgatga ttctggccgg atcgcatgag
     3301 tatcaggggg ccggagttct ggcaacctat ggagctttgc gatcgggagc cggtctgatc
     3361 agcttggggt tgcccgagga cgtggcgaac ggcatgacct gcgaggtgct tccggatgtg
     3421 attacgcgcc gctttccggc gaaagacgga ggctttgctc ttgatgctga acaggttcgt
     3481 gcgtttgtcg gtcagtaccg tgcggtagtg gccgggcccg ggtggggccg gcagcaggga
     3541 cggcgcacgt ctctgctggc attgattcaa aattgggccg gtgggctggt gcttgacgcc
     3601 gatgcgctca atctgctcga atcgatgacc gaactgcgcg gccatggttc cgatctggta
     3661 ttgacgcctc acctgggtga gatgtcccgc ctgacgggaa aatcgattgc cgtgttgaaa
     3721 gacgatcttc ctggagccgc tagagaactg gctgaacgtg ctcaggcggt ggtactattg
     3781 aagtcggctg tcacggttat cgcgattccg gatgggcgca tctttctgct gtcgcgtccc
     3841 aacagtggtc tggcgcgcgg cggagcgggc gaccttcttg ccggtctggt cggtggtttc
     3901 atggcccaag ggatacctgg gtggcaggcg gcgatcgtgg gggccttttt acactcggaa
     3961 gcgggacgta ttgcgcgtga cgaactgggg gccgatgcca tgacgatcag tgaggtggcc
     4021 tccctgattc ctcgagcttt tcgccagttg cggggcgaag tgtcgccgcc gacccagcag
     4081 gtgtaaagcg agtgaaccgg tgatcagact tctatcgttg ggctcgggaa gtcggggaaa
     4141 cgcgtccctg atcgagatcg ggcgttcgtt ctacctcatc gacgccggtc tcagttttcg
     4201 tcgcatttct cagagtctcg cgcagtgcgg gtttgacctt gaccgactgg ctggcatcat
     4261 cgtcacgcat gagcatgacg atcacgtgaa aggcctgcag actgcgctga aaaagacgga
     4321 tgtgccggtc tacgcaactc ctggaactat cgatgccttg atcgatgaca acctggacaa
     4381 caggcgtttt gtaccggtca gatctggcga agaactcgat ctgggaaaga ttcgcgtctg
     4441 gccatttccg gtcccacacg atgcggccga gccgatcggt cttcgcctcg aagcctgcgg
     4501 ccgaaccatc gggattgcct ctgatatggg gcatgtcact ccggtgattc tcgagcatct
     4561 gaccgactgt gatctgctgt gcctcgaaag taactatgat gatgatctgc tctcggtgtg
     4621 ccgctacccg gactggctca aacgccgaat tcgcggcccg cgcgggcatc tcggcaaccg
     4681 cggggtgcgt ggcattctct cgcgcctcaa acggcagttg cagcatctga tgctctttca
     4741 tatcagccag gaatcaaaca caccggaact cgttcgcgag aatgtcgaag agattctccg
     4801 cttgccgctc ctgcaggaaa cacggttgat cgtggcctct caggacgaac cgactcccat
     4861 cctggatctt ctcgccgagc agggctatgc gcgctcggcg gtgtggcgcc agcaaaggtt
     4921 taccgatctc tatgctcatg gaaatcttca tgattggtaa aatccaggag cgtccgtatc
     4981 gctctctggt cgagaactac ctgtcgcgtt gtcacggtcg cctgtccgtg cagctgttgc
     5041 cgtgccgcga tgatgccgaa ctcatgagac gattcggcga acgggaatgg acggtcgcgc
     5101 tggatgagcg tggcaccacg cctgattccc tgggcttttc acagtggctt tccggacatt
     5161 tcaatgccgg caggcagaag gtttctgtct ttctgggcgg ctcggcgggg cttccggccg
     5221 aaatcaagac aaagagccgc gaaaggcttg ctctttccag actgacactc aatcatcaac
     5281 tggcgttgct ggtgttcgcc gaacagttgt atcgtggcac atcgattctc ttcggggagc
     5341 cgtatcacaa ggcatgatcc atctttcccc acaggcgttc atgcgtatcg agaacttcgt
     5401 gctgaacttc tcgggtattg tcatggcgcg cttgcagcaa aaggggattc acgagcggat
     5461 tcagaagcat ctggagcggc tgaacgttca aaatgttgac gactatctgc tgctgctcga
     5521 atcgacgtcg agttcacagg ttcgtgatga gctgatgtcg cttgtcacgg tcggcgaaag
     5581 ctttttctat cgaaatccgg cacagctgca gtatcttcga caacacctgt tgccagagtt
     5641 gttcgaacgc aagcgcgcgt cgggcagtca tctattgcgg gtctggagtg ccggctgctc
     5701 aaccggagaa gaaccgtatt ctctggcgca tctgctgtcc acctatgccg gcacctatcc
     5761 ggaatacacc tgcagcatta ctgcgggaga catcaaccgt ctcaatctgc agtgggcaga
     5821 aaaaggtatt tatcgtcgcc gatcggttcg cgcgcaggtg aaagagatcg aacaattgct
     5881 cggaaagccg cttggccgtg atctcgacga cggccgcttc gccgttgatc cctcgttgcg
     5941 ggctctgatc gagtttcagt ttcagaatct gcatgatctc gaaagcttga aaaccctctg
     6001 ggggtcggat attatctttt gtcgcaatgt tctgatctac ttcgaggaga cgttcaagga
     6061 acgcctcgta cagacgtttt tccacctgtt gaacccaggt ggcatgctct tcttgggtga
     6121 aaccgaatct ctcagcggga tcgaacaggg attcaaactc gtttcctgcg gtggggctta
     6181 cggatacaga aaagaatgat tgaagaacgc tgaaccatgg aacaaccgaa gaaaattcgt
     6241 acgctgatcg ttgacgattc tgctttcatg cgcactgcca tcgagcggat gctgaaagaa
     6301 gagccgtcta tcgaaatcgt cggctctgcg gcaaacggca aagaagcggt cgaaaagacg
     6361 tttcgcctgc gaccggatgt cgtcaccatg gatatcgaga tgccggtcat ggacggcctg
     6421 catgcgttga aagagatcat gcgcctgtgt ccgacgccgg tgatcatggt cagttcaatg
     6481 acccaggaag gcgcgaaagc cacgctggat gctcttgatc tcggggcctt cgattatctg
     6541 ggaaaaccgg gcagcgggtt cacgacgaac attctgaccc tcaaggaaga actgctcaat
     6601 aaggtccgcg ctgctggcga cagcaccgct cgggtaccaa agctcagaat tgtgccgctt
     6661 gttccgaagc gtgtcacgac gaccaccgat cagatgactc tgctcgattg ggtcatcgtc
     6721 gttggagcct ccaccggtgg accaccggcg atacagaaca ttctctcggc tctgccggca
     6781 ggcttgccgg ctccgatcat cattgcccaa catatgccgc ggtcgttcac cagtgcctat
     6841 gccatgcgtt tgaatacgct ctgcaacttg cgagtcaaag aagcgtctga tggcgaggtg
     6901 ctgcaacgat cggttgccta catttgccca ggcgatttcc agacccgatt caaacgacgc
     6961 gaagataatc ggtactactt ctcgatgact tcgaacgacg aagaaaaggc gcgatttgcc
     7021 ccgtgtatcg acaacctgtt cttctcggcc gccgagaact tcggtcgaaa aacactcggc
     7081 atcattctga ccggcatggg agaagacggg gttcgtggcc tgaagaacat caaactcgtc
     7141 ggcggtttga ccctggctca ggaccgcgcg acttcggtcg tgtatggaat gccgcgggcg
     7201 gcgctcgaac acggagcggt gactcgcatc atcgcgttgc ccgatattcc gggcgaaata
     7261 gaattgaccc tcaagagctg aggcggtcgc tgctgcagat actgaaaccc gggtggtgcc
     7321 cgggtttctt tttctatcga gaatcaacga ttttctgtcg ataactggta cggatctgca
     7381 aacaaggagg atccgtatga aaaatcgaat cattccgctc ctgcaggact actcaagctc
     7441 gctgctcggg ttcattcgct atctgcgcga ccgcgttccc tgcgttcctg gagcagatcg
     7501 aggaacggac ctcgactctg atgaggaaga tttccgctcc tgaccgcgtt catcgtgggg
     7561 cgaggcaaaa aaacggcc
//
LOCUS       DAORHD010000048         7532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9447_length_7532_cov_4.279524, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000048 DAORHD010000000
VERSION     DAORHD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9447_length_7532_cov_4.279524"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKO06_01915"
     CDS             complement(<1..415)
                     /locus_tag="PKO06_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNV68424.1"
                     /translation="MIEIDGSIGEGGGQILRTSLALSMCTGQPFRISALRAKRPRPGL
                     MRQHLTAVLAAKQLCQAKTNGAEISSQDLTFEPGKVKPGEYTFAIGTAGSATLVLQTV
                     LPPLLTAGKPSHVVLEGGTHNPLSPPFHFVRETFLP"
     gene            complement(434..1540)
                     /locus_tag="PKO06_01920"
     CDS             complement(434..1540)
                     /locus_tag="PKO06_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNV68425.1"
                     /translation="MLNLVSIVTVRYQELGLRFRRNQLTEVLKPGWYVAFDLLGERIE
                     VISEKDLLLENEELEQILRTGLLDDAVEGVRLTDTERALLWVDGRFERVLTPGVYAIF
                     KRLRQVKVEKLSLEDPLFENSRAFTVARAAGSEVALTSVQVEQGQVGLYFRDGRLVRE
                     LAPGFYAFWKNIATVKIVPVDLREKQLDLSGQEIITADKVTLRLNAVLTYQVTDARLS
                     VQVAENAEQALYREGQLVLREAVGSRTLDGILNEKDALAQSITAALKQKANRFGLAIV
                     SFGIRDIILPGDMRELMNRVVAAQKEAEANQIVRREETAATRSQCNTAKMMEQNPVLM
                     RLRELEVLERIAEKSQLKLVLGEKGLTERVMNML"
     gene            complement(1887..1961)
                     /locus_tag="PKO06_01925"
     tRNA            complement(1887..1961)
                     /locus_tag="PKO06_01925"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1926..1928),aa:Met,seq:cat)
     gene            2190..3782
                     /gene="rtcR"
                     /locus_tag="PKO06_01930"
     CDS             2190..3782
                     /gene="rtcR"
                     /locus_tag="PKO06_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003161424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HNV68426.1"
                     /translation="MNQIVVGFLGTTLDRRGRTSRWEHWRPSVCLCQHEELLITRFHL
                     LYAERDLALARQVKEDILSVSPETDVVLELLEFRNPWDFEEVFATLLDYSQRFHFDRE
                     SEELLLHITTGTHVAQICLFLLCEAGFFPGKLLQTGPGRNSHPPGSYSLIDLDLSRYD
                     SIARRFAVRTNDDISILKSGIQTRNQRFNHLIAEVEKVTLRSRDPILLLGPTGAGKSR
                     LARQIYERRKQRGLISGGFIEVNCAMLRGDTVQSSLFGHRKGAFTGATTDRPGLLKQA
                     DGGLLFLDEIGELGPDEQAMLLRAIEEKTYLPLGSDHEVKSDFQLICGTNRDLKREVA
                     VGGFREDLYARIKFWSFRLPALKERLEDVEPNIDFELQNRSREAGRPITLSREARERY
                     LRFAVGPQAVWPANFRDLNNSIARMALLAEGNRIAVDDVQREIERLKNEWAETSPDDS
                     SDELETLLGTKAAAELDLFDRVQLLEVISVCRRCPTLAEAGRRLFAMSRQRRRQANDT
                     DRLRKYLLKFHLTWEAITAMSA"
     gene            4014..4772
                     /locus_tag="PKO06_01935"
     CDS             4014..4772
                     /locus_tag="PKO06_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNV68427.1"
                     /translation="MRAKPRTSAHDHIQTLTDLKQRLQSLARPRHAELQLRFFKTGPG
                     EYGEGDRFLGLDVPTQRALAREFRHLPLDAAKVLLLSPWHEERLVALLLMIGHYEKAA
                     PKEREALTEFYLSHARYVNNWDLVDASAHLLAGPTFPATRTKRLETLARSGMLWERRI
                     AVVATFHHIRQNQFEPTLRISDILLHDTHDLIHKAVGWMLREVGKRDLAVLEEFLKPR
                     YAKMPRTMLRYAIERFPEKRRQAYLKGKIGALQP"
     gene            4889..6157
                     /locus_tag="PKO06_01940"
     CDS             4889..6157
                     /locus_tag="PKO06_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV68428.1"
                     /translation="MKRLVQWSLFLFLIACACPVYALAEWTVIAYFVAEEGDKQSTLE
                     DAQIRNINQMATPGSSDQVNLLVQMDRGQGLSALMKETYADPNYSGAKRYRLAGGKWN
                     ALEKLGEVNMGSPYALWECLKWAAEKYPAKRYALFINSHGSGVFSWRGTGATGSTSPG
                     AVEFNPDRFVAYDTADNDCLTVFEVAAVLRAFRKKLNAGRPMSLIGFDACYSGCIEAL
                     YQFRDSTEVMMASPSETAMTGFPYGSIARALHAVPALDPEKLAEMATEAYIKNTNSSN
                     AQVMGAWRATKAGRLANAIGQLAFEIDQAMQQTGKKFGVQSIVACGDKERYWDIRRVA
                     QSIKTGNASFNGATNLTTIQQLAGDVENAVNDARISVWYDGRFAEGKVGGLSIAWPGA
                     AEYQQWRAFYKALEFSADTHWDEMLDRREL"
     gene            complement(6266..6898)
                     /locus_tag="PKO06_01945"
     CDS             complement(6266..6898)
                     /locus_tag="PKO06_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68429.1"
                     /translation="MELAGNLCLQPETPPSPDRSTSSGETSSTVNTLVRTIQTLKRRR
                     AALPDELSYLAVNESSRQWWKELSDFWAAPASVRGYHRPNQTQGDHSRRVFKLACRHW
                     HRVPWDHLRRNRRVCRSFLRAALFHDVGKITDRRRHEIESFHWMLPKDQLAAFFVLHH
                     MGRWSCINDAEKNDLLERAGVLHLSTPEHLWLADLLCAADFTEACCWDIT"
     gene            complement(7205..>7532)
                     /locus_tag="PKO06_01950"
     CDS             complement(7205..>7532)
                     /locus_tag="PKO06_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV68430.1"
                     /translation="VSTADIGEKMEIPYRFLRRITRKLVEKGFLEAQRGKTGGVYLVV
                     EPKKISLLDVMQIFDQRGITVNACCTPKRGCRRDQHCPVHPHFETVQKEFHRSLQKMS
                     FARLAK"
BASE COUNT         1511 a         2254 c         2196 g         1571 t
ORIGIN      
        1 ccggcagaaa cgtctcgcga acgaagtgaa acggaggaga cagcgggttg tgcgtgccac
       61 cttcgagaac cacgtgagaa ggcttgccgg cggtgagcag aggcgggagg accgtctgca
      121 gaaccagcgt ggcactgcca gctgtgccga tggcgaacgt gtactcgcct ggcttcacct
      181 tccccggttc gaaggtcagg tcctgcgaac taatctcggc cccgttcgtc ttcgcctgac
      241 agagttgttt ggcggccaga accgccgtca ggtgctggcg catcaggccc gggcgcgggc
      301 gtttcgcccg cagagcgctg attcgaaacg gctgacctgt acacatcgaa agcgcgagag
      361 acgtgcgcag aatctgcccg ccaccttcgc caatgcttcc atcgatttca atcatggttg
      421 ttctccctct tactcacagc atgttcatga cccgttcggt caggcccttc tcacccagca
      481 ccagcttcaa ctgcgacttt tcggcgatgc gttccagtac ttcgagttcg cgcagacgca
      541 tcaaaaccgg gttctgttcc atcatcttcg ccgtgttgca ctgactgcgg gtcgcagccg
      601 tctcttcgcg ccgcacgatc tggttggcct cggcttcctt ctgcgccgcg accacccggt
      661 tcatcagttc gcgcatgtcg cccggcagaa tgatatcgcg aatcccgaac gagacgatag
      721 ccagtccgaa ccggttcgcc ttctgcttca acgccgccgt gatactctgg gccagcgcgt
      781 ccttctcgtt cagaatgcca tcgagtgtgc gactgcccac ggcttcgcgc aacaccagct
      841 gtccttcgcg atacagggcc tgctcggcgt tttccgcaac ctgcaccgac aggcgcgcat
      901 cggtaacctg atacgtcaga accgcgttca ggcgcaacgt caccttgtcg gcggtgatga
      961 tctcctgacc cgacaggtcg agctgtttct cacgcagatc aaccggaaca atcttgaccg
     1021 tcgcgatgtt cttccagaac gcgtagaacc ccggcgcgag ttcgcgcacc agacggccat
     1081 cccggaagta gaggccgacc tgaccctgtt cgacctgcac gctggtcaga gcgacctcac
     1141 tgccggccgc ccgggcgacc gtgaaagcgc ggctgttctc gaacagcgga tcctcgaggc
     1201 tcagcttctc gaccttcacc tggcgcagac gcttgaagat cgcatacacg cccggggtca
     1261 gcacccgttc gaaccggccg tcgacccaga gcagggcccg ttcggtatcg gtcaggcgaa
     1321 cgccttcgac cgcatcgtcc aggagaccgg tgcgcagaat ctgttccaat tcttcgtttt
     1381 cgagcagaag atctttttcg gagatcacct cgatgcgttc gcccagcaga tcgaaggcga
     1441 cataccagcc tggcttgagc acctcggtca gttgattgcg gcgaaatcgc agtcccagtt
     1501 cctgatagcg taccgtgacg atggaaacca gattcaacat agacgttcct ccttccgaaa
     1561 gtcgatcttc gactcagttc attcattcga cgctcatcgg caccatctgc gccgatattg
     1621 tgtgcgagag ccgtcagagc gggctttccg tcgtttccgc gcctcggcga tggagtgaag
     1681 tttgaggggt tcgggctgaa ggtcaccccc tgaacgcggg cttcaagggg ctcgaacgcc
     1741 ggtatccgct tcactgcaaa accgcctctg gtgctgcttt cctggctcga ccgcgagaac
     1801 gggggttgtc gggcccccca tgcggggaga gcgggtgggc tggaaccacc ggatcgcctg
     1861 ctcctcgggc cagttcgggt gtcttgcgac actggtgcgg ggttcgaacc cgcgacaggc
     1921 agttgatgag actgctgctc taccattgag ctaaccctgc cggcctcctc tttcgatgcc
     1981 gctctccgga actccctcgc ctcaccggcg ggagccgggc ggtcgattca gccgacactc
     2041 tttgtcattg caatcgcaat gccaggttga cgaccgtggc acgcgaatcg cccgcatcag
     2101 ctctggaagc cgatactcga ggttttagcc ggttttgtcg gtccaatgtt tctcgcgaaa
     2161 catctaatgt gctatactat attctagtca tgaaccagat tgtcgtcggc tttctcggca
     2221 ccaccctcga ccggcgcggc agaacgtcgc gctgggaaca ctggcgcccc tcggtctgcc
     2281 tctgccagca cgaagaactg ctgatcaccc gctttcatct gttgtatgcc gagcgagatc
     2341 tcgcactggc gcgccaggtc aaagaagaca ttctttcggt ctctcccgag accgacgtcg
     2401 tgctcgagct tctcgagttt cgcaacccgt gggacttcga ggaagtcttc gcgacgctgc
     2461 tcgactactc tcagcgattc catttcgatc gcgagtctga agagctcctg ctgcacatca
     2521 ccaccggaac gcatgtcgca cagatctgcc tctttctcct gtgcgaggca ggcttcttcc
     2581 cgggcaagct tcttcagacc ggcccgggtc gcaacagcca ccccccgggc agttactcgc
     2641 tcatcgatct cgatctttcc cgctatgaca gcattgcgcg tcgttttgcc gttcggacga
     2701 atgacgatat ctcgattctg aagtcgggca ttcaaacccg caatcagcgt ttcaatcacc
     2761 tgatcgccga agtcgagaag gtcactctgc gcagtcgcga cccaattctc ctgctgggtc
     2821 cgaccggcgc ggggaaatcg cgtctggcgc gtcagatcta cgagcggcgg aaacagcgcg
     2881 gactgatcag cggcggcttc attgaagtga actgcgccat gttgcggggc gatacggttc
     2941 agtcgtctct cttcgggcat cgcaagggcg cgttcaccgg cgcgaccacg gaccgccccg
     3001 gtctgctcaa acaggcggat ggcggcctgc tgtttctcga cgagatcggc gagctcggcc
     3061 ccgacgaaca agccatgctg ttgcgcgcca tcgaagagaa aacgtacctg ccgctcggtt
     3121 ctgatcacga ggtgaaaagc gactttcagc tgatctgcgg tacgaatcgt gacctcaagc
     3181 gggaggtcgc cgtcgggggg ttccgcgagg acctgtacgc ccgcatcaag ttctggagct
     3241 ttcgcctgcc cgccttgaaa gagcgtctcg aagacgtcga acccaatatc gacttcgaac
     3301 tgcagaaccg ctcccgcgaa gccggcaggc cgatcacgct cagtcgcgaa gcccgcgaga
     3361 gataccttcg tttcgcggtg ggcccacagg ctgtctggcc cgccaacttt cgcgatctca
     3421 acaattcgat cgcccgcatg gctttgctcg ccgaaggcaa tcgaatcgcc gtcgatgatg
     3481 tccagcgtga gatcgagcgc ctgaagaacg aatgggccga gaccagcccg gacgattcga
     3541 gcgacgaact cgagactctg cttggcacga aagccgcggc cgagctcgat ctcttcgacc
     3601 gcgtccagct tctcgaggtc attagcgtct gtcgccggtg cccgacgctc gccgaggccg
     3661 gccggcgcct gttcgccatg tcacgtcagc gccgacggca ggccaacgac accgaccgcc
     3721 tgcgcaagta tctgctgaag ttccacctga cctgggaagc gatcaccgcc atgtcggcat
     3781 gagtggccac cctggcgtac tggttcaggg gcaattgatt ctctgtcttg ggttttcccc
     3841 ttgtcatcgc gcaacgattc gccggccggc tcacatgttg tgctcacgcc aggaggaaaa
     3901 cgagacagaa tcaaaattcc ctggttctgg ttgcggcagg cttttctttg cacggtcggt
     3961 gcgctaccat gcgtctggaa cccatcactt cgcggcgatc ttcaggaggg ctcatgcggg
     4021 cgaaaccacg aaccagcgca cacgatcaca ttcagacgct gacagatctg aaacaacgct
     4081 tgcagagtct ggcgcgaccg cgccacgcgg aattgcagtt gcgctttttc aagaccggcc
     4141 ccggcgagta tggagaagga gaccgctttc tcgggcttga tgtgccgact cagcgcgccc
     4201 tggctcgcga gtttcggcat ctgccactcg atgccgccaa ggttctgctt ctttcaccct
     4261 ggcatgaaga acgcctggtc gcgcttctgc tcatgatcgg gcattacgag aaggctgccc
     4321 ccaaagagcg cgaggccctg accgagtttt atctgtcgca tgcccgctac gtgaacaact
     4381 gggatctcgt cgatgccagc gctcacctgc tggccgggcc gacgtttccc gcgacgagaa
     4441 cgaaacgcct cgaaaccctg gcccgctccg gcatgctgtg ggaacgccgc atcgcggttg
     4501 tcgccacctt tcaccacatc aggcagaacc agttcgagcc gactctgcgg atcagcgaca
     4561 ttcttttgca cgatacgcat gatctgattc acaaagcggt cggctggatg ctccgcgaag
     4621 tcggcaaacg tgacctggcg gttttggaag agtttctgaa gccgcgatac gccaagatgc
     4681 cgcgcaccat gctacgttat gcaatcgaac ggttccctga gaaacggcgc caagcttacc
     4741 tgaaagggaa gatcggcgca ttgcagccct gatctgcacc cgagactgcg tagttcaccg
     4801 tgcgatgctg ccgataaggg cggactgctc gcaccttttc ccctctcaat gatacactga
     4861 gtacactttg actcatcgag gtgactccat gaagcggctg gttcaatggt cgttgttcct
     4921 ttttctgatc gcgtgtgcat gcccggtgta cgcgcttgcg gagtggaccg tgatcgccta
     4981 ctttgtcgca gaagagggtg ataaacagtc gacactcgaa gatgctcaga ttcgaaacat
     5041 caatcagatg gcgactcccg gctcgtctga ccaggtgaac cttctggtgc agatggatcg
     5101 aggtcaggga ctgagtgcgc tcatgaagga aacttatgct gatcccaatt acagcggggc
     5161 caagcgctat cgcctggccg gtgggaagtg gaacgcgctg gaaaagctcg gcgaagttaa
     5221 catgggatct ccgtatgccc tttgggagtg cctcaagtgg gccgcggaaa agtacccggc
     5281 gaaacggtat gccttgttca tcaacagtca tggcagcggg gtgttctcct ggcgtggaac
     5341 gggagcgacc ggcagtacca gtcctggtgc cgtcgagttc aatcccgatc gttttgtggc
     5401 ctatgacacg gccgacaatg attgtctcac ggtcttcgag gtggcggcgg tgcttcgtgc
     5461 ctttcggaaa aagctgaacg ccggtcgacc gatgagcctg atcggttttg atgcgtgtta
     5521 ctccggctgc atcgaagcgt tgtatcagtt ccgtgacagc acggaagtga tgatggcaag
     5581 cccatcagag acagccatga cgggcttccc ctatggttcg attgctcgtg ctctccatgc
     5641 cgttcctgcg ctcgatccgg aaaagctggc ggaaatggcc acggaggcat acatcaagaa
     5701 caccaacagc tcaaatgctc aggtgatggg cgcgtggcgg gccacgaaag ccggtcgact
     5761 cgccaatgcg atcggacagc tggctttcga aatcgatcag gccatgcagc agacgggaaa
     5821 aaagttcggt gttcagagca ttgtggcctg tggtgacaag gaacggtact gggacattcg
     5881 gcgggtggcg caatcgatca agactggaaa tgccagcttc aatggggcga ccaatctgac
     5941 gacgattcag caacttgcgg gagatgtcga aaacgcggtg aatgacgcac gcatatccgt
     6001 gtggtacgac ggccgttttg ccgagggaaa agtcggtggc ttgtcgatcg cgtggcccgg
     6061 cgctgccgag tatcaacaat ggcgcgcgtt ctacaaagcc cttgagttct cggctgatac
     6121 ccactgggac gaaatgctgg atcgacgaga gctgtaacga ggagtatcaa cacacacgcc
     6181 gcccggaaga ctcccgggcg gcgtgtgtgc tttcttgcaa aaagaagcgg gatgggaacg
     6241 tcgcgacgtt cctgagagcg tcagttcagg tgatatccca gcagcaggct tcggtgaagt
     6301 cggcggcgca cagcaggtcg gcgagccata gatgttcagg cgtcgagagg tgcagcactc
     6361 ccgcccgttc gagcaggtcg tttttctcgg catcgtttat acaggaccag cgtcccatat
     6421 gatggagtac gaaaaacgct gccagctggt cttttggcaa catccaatga aagctttcga
     6481 tctcgtgacg ccgacggtcg gtgattttgc cgacatcgtg gaacagggct gctcgaagaa
     6541 aacttcggca gacacggcga ttgcggcgca gatgatccca cggtacgcga tgccagtggc
     6601 ggcaggccag tttgaatact cgccgcgaat ggtcaccctg tgtttgattc ggtcgatgat
     6661 agccgcgaac tgaagcgggc gcggcccaga aatcactgag ttctttccac cattgacgcg
     6721 aactttcgtt caccgcgagg taggacaatt catcggggag ggctgctcgc cgccgcttga
     6781 gggtctgaat cgtgcgcacc agcgtgttca cggtggaact tgtctctcca ctcgaggtcg
     6841 aacgatcggg tgagggaggg gtctcgggct gcagacacag gtttccagca agttccatgg
     6901 cgcactctca ctcgtttgaa ggtgggcaga tgctagcacg tgctcaggtc atgcacaaga
     6961 gcttgtgttg aagggcgtga gagccgcgag gctggtgaaa acccgcatgc catggctcct
     7021 cactcacgct gtaccgagcc ggctgtgccc cggtcgaaag tcgcgtcgcc atcatccttt
     7081 gagaaatccg atcctgcatt caagccattc ttcaagaaaa agcttgattc tctcaaatgc
     7141 agatcagcag ctaaaaagaa aaaccccgga ccaggacatc cggggaggtg atcgaaaaaa
     7201 gggcttactt ggccagccgg gcaaaactca ttttctgaag gctacgatga aactctttct
     7261 gcaccgtctc aaagtgggga tgcaccggac agtgctggtc gcggcggcac ccacgcttgg
     7321 gggtgcagca ggcgttgacc gtgatgcccc gttgatcgaa gatctgcatg acgtcgagca
     7381 gagagatttt cttgggttcg accacaagat acacgccacc cgttttccca cgctgagctt
     7441 cgaggaatcc cttttccacc agtttccggg tgatgcggcg caggaaccga taggggattt
     7501 ccattttctc gccgatgtca gctgttgaaa ca
//
LOCUS       DAORHD010000049         7504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9499_length_7504_cov_3.531615, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000049 DAORHD010000000
VERSION     DAORHD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7504
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9499_length_7504_cov_3.531615"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="pgk"
                     /locus_tag="PKO06_01955"
     CDS             <1..426
                     /gene="pgk"
                     /locus_tag="PKO06_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNV68431.1"
                     /translation="TLLLPVDVVCAPEIKEGVTVKTVDLKSIPADQLGLDIGPKTIEL
                     FAAELKKARTVLWNGPMGVFETKPFDAGTRKVAEALTTLKATTVVGGGDSVAAIEQMG
                     AADKVSHVSTGGGACLEYLEGLVLPGIEIFSRDIKSMMK"
     gene            437..1189
                     /gene="tpiA"
                     /locus_tag="PKO06_01960"
     CDS             437..1189
                     /gene="tpiA"
                     /locus_tag="PKO06_01960"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNV68432.1"
                     /translation="MARRPLIAANWKMHKTQAEARAFMTAFIPKVSAVKARDILVCPP
                     FPLISVVDAARRDTNIFLGAQNMYFEEKGAFTGEVSPLMLKDLHCEFVILGHSERRWV
                     FNESDELINRKTLAAVEKGIIPILCVGEKLDEREKNLTEQVILNQCEKGLHGLNEKTV
                     AGMVIAYEPVWAIGTGKNASTKDAKDAIALIREFVHSRFGADAAAKVRILYGGSVKPE
                     NFKTYMDQEGIDGGLVGGASLEAESFAKLVMY"
     gene            1298..3067
                     /locus_tag="PKO06_01965"
     CDS             1298..3067
                     /locus_tag="PKO06_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV68433.1"
                     /translation="MGIRLKLGLGFAALIVLLSSLTWYWGARSLGLSIDSSDSLRLVN
                     LREQWQAQWRHEQDTLKGLGAEVAKAFAPYLIPVDDVADGTRSQTSEAVPVTPATLEV
                     ARLIADRLRAHLRLEWIELRHEGRPLLYPGVTMADLPENPPPEPIRLATDGPLSLQGY
                     LVAVQPVMGTPCEVVLMRRSGSPVVPLASLWDTHGYLAGEAGVADDLWYRRELARSDP
                     GEPVVSQILHQGRLYRLRVDERTTGSKRLLVGYEADASTLTRAGVSDLLIQLAMLQTA
                     GFLLLGYFLGRRLFQPLERLREGMERVAAGQWQKIPEEGGDEIGEVARSFNRMVQDLA
                     LAQTRLLEIQRELVAKEKMAVLGRFSAGVAHEINNPLGTILVCAGNARESLDSAGTVD
                     AEDLASIVEATKRCRQIVDSLLNYARNRPPERSQVELSELVRDAVVQLKPERDAASIL
                     VCAAEVPETRLSVDGRAMVQVLRNLLANACDAVQGLSSPRVEIRCRAPEPGWVEVSVL
                     DNGPGLPGGENHLFEPFYSTKSKGTGLGLAISQSIVESHGGRLWAERLPEDLTAFRFT
                     IPTFAGGGDPLSRMDRHRSDTVS"
     gene            3064..4428
                     /locus_tag="PKO06_01970"
     CDS             3064..4428
                     /locus_tag="PKO06_01970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV68434.1"
                     /translation="MSKKILLIDDEVKLLQALKKALEREGYEVFGFADPAGALEFALQ
                     RELDLVISDIRMPGLSGLELMSRLGASGRAVPTMLMTAYSSVETAVTAMKMGARDYLI
                     KPFEVGDFKAAVRKVLAAGADETKAEEPESKYEIVGHSAAIKQVLDLIGQVADTESTV
                     LLQGESGTGKELVARALHLCSRRSDRPFVPVNCSTLPETLFESEVFGHVRGSFTGAVG
                     DKIGLLVEAQGGTLFLDEIGDLAPANQAKLLRVLQDGTFKRVGDARTMTADVRVLAAT
                     NRQLRQEVAAGRFREDLLYRLAVVEIRLPPLRERLDDLHELAGHFLRRAAVKHHRPQP
                     VFDEALVRHLATHSWPGNIRELENYLERAVILTRDARLTPQSVPLPKTTEESPTASRE
                     QPLPGVDGSLGETIDRIEQELVMRALESAQGNHSRAAEMLGVTRQNLHYKIKKYRLQD
                     RGRS"
     gene            4425..5657
                     /locus_tag="PKO06_01975"
     CDS             4425..5657
                     /locus_tag="PKO06_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68435.1"
                     /translation="MTNWRRWLIVACSALLFCLPCVGQGIASWTMEKSASGVVLLFAQ
                     PVTLDGVRLPVRAVELWGPGRRGTVAALKSDHAVGIAVPDIPDQTLFGVISDASASRL
                     DITPVYAEIEEPASGSGVGSSADLLDRIKVLGTSAEQRRLEVLLSQNLQEEVEWQRRR
                     GCFSCHRLLPLGLAVQSAVEQKIALPHDSVRELLQNLTDWQSGDGSWFFPRASRYGRV
                     TATLTALAVLGWWEEITTTDGSASLLRGLGFLAGVQREDGGVAFDFTYPPVFHGRAAA
                     GWLMLNGLVFGRREVERAGHAFPSRFVEMQMRLCEWFAQVEDHAWKKYLFAMMASRLD
                     SQVVAPARESLPDFLVLEVRQLGFPPELPISSLVNFALRHEGRTLLPRRLALADRTSS
                     TTRFWDLLDSLVREPDPW"
     gene            5759..7150
                     /locus_tag="PKO06_01980"
     CDS             5759..7150
                     /locus_tag="PKO06_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV68436.1"
                     /translation="MFLFCLCWVSSILAAAPASWAGKSSQVSAVRETAVSAPETRTGS
                     QAAGQSSPIDPSIPRDIVRDFEVLRQQLAQHPRDVAILNSLGILYARVGQLNDALSVW
                     QRALEYDPRYVHLYNNIGSALKTQKRFAEARQVFQRGLQVAPSYWIHYNLGLLEKELG
                     RLADAAICFQMALRMNPKFEPAQQKMREIAAAAAGYPVDGNSSMRTELPFPFEVKPPV
                     SVEGTPQTEDAEDEDQGPPVRRWGGTNRGSSDVGPVSTVAECVAVLGRHQPGRFGKTI
                     ALTFDDGPHPSYTTQLLEYLRSQGVRATFFVLGSRAETYPHLITRMSDEGHELANHTW
                     SHRSLVNQSQSAALSEMRRTSELLSGLTGRSPRLVRPPYGHTNAKVARLIHGQGWHQI
                     MWDADSRDWAGGSSQAMLQRVMRQFSPGGIVLFHDIHPGMLRVLPVLIPALKQCGYRF
                     VTVSELMALDPAS"
     gene            7156..7290
                     /locus_tag="PKO06_01985"
     CDS             7156..7290
                     /locus_tag="PKO06_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68437.1"
                     /translation="MSAALFDFCLILLLLGFFLYGLREWDHLLADPEEDQREKAGGAA
                     "
BASE COUNT         1464 a         2005 c         2375 g         1660 t
ORIGIN      
        1 accttgctgc tgccggtcga cgtggtctgc gcccccgaga tcaaggaagg cgtgaccgtg
       61 aagacggttg atctgaagtc gattcctgcg gatcagctgg gtctggatat cggtccgaag
      121 accatcgagc tgtttgccgc tgaactcaag aaagctcgca ccgttctctg gaatggcccg
      181 atgggggttt tcgagaccaa gccgtttgac gccggtaccc gcaaagttgc cgaagcattg
      241 acaaccctga aagccacgac ggtggtcggc ggcggagatt ccgtcgctgc gatcgaacag
      301 atgggtgccg ccgacaaggt gagccacgtc tcgaccggtg gtggcgcatg tctggagtat
      361 ctcgaaggcc tggtgctgcc cggcatcgag atcttttcgc gcgatatcaa aagtatgatg
      421 aaatgaggtg actcgtatgg cacgtcgacc gcttatcgcg gctaactgga agatgcacaa
      481 gacccaggct gaggcccggg ccttcatgac cgcttttatt ccgaaagtgt cggcggtgaa
      541 agcccgtgat atcctggtct gcccgccatt tccgctgatc tcggtggtcg atgccgcccg
      601 ccgcgacacc aacatttttc tcggggcgca gaacatgtac ttcgaggaaa agggggcgtt
      661 caccggcgaa gtttcgcctc tgatgctgaa agacctgcac tgcgagtttg tcattctcgg
      721 acattccgaa cgccgttggg tgttcaacga gagtgacgaa ctcatcaatc gcaaaacgct
      781 cgcggccgtc gagaaaggca ttattcctat cctctgcgtc ggtgagaagc tggacgagcg
      841 tgaaaagaac ctgaccgaac aggtgattct caaccagtgt gagaaggggc tgcatgggct
      901 caacgaaaaa acggtggccg gtatggtcat cgcgtatgaa ccggtgtggg ccatcggaac
      961 cggcaagaac gcctcgacca aggatgccaa ggatgcgatc gcgttgattc gcgaattcgt
     1021 ccacagccgc tttggtgcgg atgcggctgc caaagtccgc attttgtacg gtggttcggt
     1081 caaacccgaa aacttcaaga cctacatgga ccaggaagga attgacggcg ggttggtggg
     1141 cggtgccagc ctcgaagccg agtcgtttgc caagctggtg atgtactagt tcccgttttt
     1201 ttctgcgctt tttccccgcg gtgccgaacg atcgcccgcg gggttttccg tttcaggagg
     1261 agacagggta taatgacggc gaaggagacg cgttgaggtg ggaattcgcc tgaaactggg
     1321 gcttggtttt gcggccctca tcgtgctttt gagttcgctc acctggtact ggggtgcgcg
     1381 aagccttggg ctttccatcg attcgtctga cagtctgcgt ctggtgaatc tgcgcgagca
     1441 atggcaggcg caatggcgcc atgagcagga tacactcaag ggtctgggtg ccgaggtcgc
     1501 caaggctttt gctccgtatc tcatcccggt tgacgatgtc gcagatggca caagatctca
     1561 gacttccgag gccgtgccgg tgacgccggc gacactggaa gtggcgcgcc tgattgcgga
     1621 tcgtttacgg gcgcatttga gactcgagtg gatcgaactt cgccacgagg gtcgaccgtt
     1681 actgtatccg ggcgtcacca tggcggatct cccggaaaat ccgcccccgg aaccaatccg
     1741 actggccacc gatggacctt tatccctcca gggatacctc gtcgccgtgc aaccggtgat
     1801 gggcacaccc tgcgaagtgg ttctcatgcg acgttctggc tcgccggtgg tgccactggc
     1861 cagcttgtgg gatacgcacg ggtatctggc tggcgaagcc ggagtggccg atgatctctg
     1921 gtatcggcgc gaactggcga gatctgaccc gggagaacct gtggtgtcgc agatcttgca
     1981 tcagggtcga ttgtacagat tgcgcgtcga tgaacgaacg actggcagca agaggctgct
     2041 tgtgggatat gaggccgatg cctcgaccct gacccgtgct ggggtcagtg atctgctgat
     2101 tcagcttgcc atgctgcaaa ccgccggttt tctgctgctg gggtattttc tcggtcgccg
     2161 cctgtttcag ccgctcgagc gcttgcgcga ggggatggaa cgagtggcgg cgggacaatg
     2221 gcagaagatt cccgaggaag ggggcgacga aattggtgaa gtcgctcgca gtttcaaccg
     2281 catggtgcag gatcttgctc tggcgcaaac gcgactgctt gagattcagc gggaattggt
     2341 ggcgaaagag aaaatggcgg tgctgggacg gttctctgcg ggagttgcac atgaaatcaa
     2401 taatccgctg ggtaccattc tggtctgtgc cggcaatgcg cgcgagtcac tcgacagtgc
     2461 aggaacggtc gacgcagaag atcttgccag cattgtcgag gccacgaaac gctgccggca
     2521 gattgtcgac tcactgctga actatgcgcg taatcggcct cctgagcgca gtcaggtcga
     2581 gctgagtgaa ctggtcagag atgcggttgt ccagttgaag ccagaacgtg atgcggcatc
     2641 gatcctggtc tgtgcggcag aggttcctga gacacgtctg tctgttgatg gccgggcgat
     2701 ggtgcaggtg ttgcggaatc tgctggccaa tgcctgtgat gccgtgcagg gcttgtcttc
     2761 accgcgagtc gagatacgat gtcgtgcgcc tgaaccggga tgggtcgagg tgagtgttct
     2821 ggacaacggt cccggacttc ccggaggcga gaatcatctg tttgagccat tctattcgac
     2881 caagagcaag ggaaccggtt taggactggc gatttcgcag tcgatcgtgg aaagtcatgg
     2941 tggccgcctg tgggctgagc gtctgcctga agacctcacc gcattccgtt ttaccatacc
     3001 gaccttcgcg ggcggcggag accctctttc ccgcatggat agacatcgga gtgacacagt
     3061 atcatgagta agaaaattct gttgattgac gatgaagtga aactgctgca ggccttgaaa
     3121 aaggcgctgg aacgagaagg atatgaggtc ttcggttttg ctgatccggc gggagcgctg
     3181 gaattcgctc tgcagcgcga acttgatctg gtgatttccg atattcgcat gccgggtctt
     3241 tccggtctgg aactcatgag cagactggga gcttctggac gagccgtgcc gaccatgctg
     3301 atgaccgcgt attcgtccgt ggaaaccgcg gtaaccgcca tgaagatggg ggctcgcgac
     3361 tatctcatca agccgttcga ggtgggagat ttcaaagcgg cggtgcgcaa agtcctggcg
     3421 gcaggcgcgg atgaaaccaa ggcggaagaa ccggagtcga aatacgagat cgtgggccat
     3481 tccgcagcga tcaagcaggt gctcgatctg atcgggcagg tggctgatac cgagagtacc
     3541 gtcttgctgc agggcgagag cggtacggga aaggaattgg tggcacgggc tctgcatctg
     3601 tgttctcgaa gaagcgatcg cccgtttgtc cccgtgaact gtagcacgtt gccggaaact
     3661 ctttttgaaa gtgaagtgtt tggtcatgtg cgtggcagct tcaccggagc cgtcggcgac
     3721 aagatcggtc tgctggtcga ggcgcagggt ggcacactgt ttctcgatga aatcggggac
     3781 ctggcgccgg cgaatcaggc caaactcctg cgtgtgctgc aggatggaac gttcaaacgt
     3841 gttggtgacg cacgcacgat gaccgcggac gttcgtgttc tcgcggccac gaatcggcag
     3901 ttacgccagg aggtggctgc cggccgattt cgtgaagatc ttttgtaccg gttggcagtg
     3961 gtagaaattc gcctgccacc gctgcgagaa cgacttgatg atctgcatga gctggcgggc
     4021 cattttctgc gacgtgcggc cgtcaaacat catcgaccgc agccggtctt cgacgaagcg
     4081 ctggttcgac atctggcaac acactcctgg cccggaaaca ttcgggaact ggagaattac
     4141 ctcgaacggg ccgtgattct gactcgtgat gcccggctca cgccccagtc cgtaccgctt
     4201 ccgaaaacga cggaagagtc gcccactgcc tctcgcgagc agccgcttcc cggagtggac
     4261 ggatcgctgg gtgaaaccat tgatcgtatc gagcaggaac tggtgatgcg agcgctcgag
     4321 agcgcccagg gaaaccactc acgagcggcg gaaatgctcg gagttacccg acagaatctc
     4381 cattacaaga tcaagaaata ccgattgcag gatcgaggac gttcgtgacg aattggcgcc
     4441 gctggctcat cgttgcatgt tctgccctgc tcttctgcct cccttgcgtt ggtcagggta
     4501 ttgcctcctg gaccatggaa aagtcggctt ccggggtcgt gctgctgttc gcgcaaccgg
     4561 tgaccctcga cggggtccgg ctgcccgttc gggcagtcga gttatgggga ccgggccgac
     4621 gtggaaccgt ggcagcgctg aaatccgatc atgcggttgg aatcgcggtt cccgatattc
     4681 cggaccagac cctgttcgga gtgatctcgg acgcgtccgc gagtcgtctc gacattactc
     4741 ccgtgtatgc cgagatcgag gaaccggcga gcggatcagg cgttggctca tctgctgatc
     4801 tgctcgatcg tatcaaggtt ctgggcactt ctgccgaaca gcgccggctc gaagtcctgc
     4861 tcagccagaa cctgcaggag gaggtcgagt ggcaacgtcg ccgtggctgc ttctcgtgtc
     4921 atcgtctgtt gccgcttggg ctggctgtgc agagcgccgt cgagcagaaa atcgccctgc
     4981 cgcacgactc ggtgcgcgaa ctcctgcaga acctgactga ttggcagagt ggagacggga
     5041 gctggttttt cccgcgagcc tcacgctatg ggcgtgtgac cgcgactctg actgcactgg
     5101 ccgtactggg gtggtgggaa gagatcacga cgaccgacgg gagtgcgtcg ttactgcgtg
     5161 gtcttggctt tcttgccgga gtgcaacgcg aagatggggg agtggccttt gactttacct
     5221 atcctcccgt gtttcacggt cgtgccgcgg ccgggtggct gatgctgaat ggtctggtgt
     5281 tcggtcgtcg agaagtcgag cgcgccggtc acgcatttcc gtccaggttc gtcgaaatgc
     5341 agatgcgctt gtgtgaatgg ttcgctcagg tcgaagatca tgcctggaaa aagtacttgt
     5401 ttgcaatgat ggcaagccgc ctcgattctc aggtggtggc cccggccaga gagagtcttc
     5461 cggattttct ggttcttgag gtcagacaac tcggctttcc cccggaacta ccgatcagct
     5521 ctctggtcaa tttcgcgttg cgtcatgaag gacgcaccct gttaccgcgc cggttggcgc
     5581 tggcggaccg gacgtcgtcg acaacccgtt tctgggacct gctcgacagt ctcgtgcgcg
     5641 aacccgatcc gtggtgaggg tgaaaaaaac gcggcagcct gtcgatacac ggagtcatat
     5701 gaacgcccgt tcgtgttttc cctgctcgcc gcttttcacc tgcggaaaca gactccgatt
     5761 gtttctgttc tgtctgtgct gggtgagttc catcctggca gcagctccgg catcctgggc
     5821 aggaaaatcc tctcaggtct ctgcggtgcg cgagactgcg gtatcggcac ccgagaccag
     5881 aactggcagc caggctgcag gacagtcttc accgattgat ccgtcgattc cccgcgacat
     5941 cgtaagagat tttgaggtat tgcgacagca actcgctcag catccgcgcg atgtggcgat
     6001 tctcaactcg cttggcattc tgtatgcgcg ggtcggacaa ctgaacgacg ctctgtccgt
     6061 ctggcagcgc gcgcttgaat acgacccaag atatgtgcat ctctacaaca atatcggatc
     6121 agctctgaag actcagaaac gttttgctga ggcgcggcag gtgtttcagc ggggattaca
     6181 ggtcgcaccc tcctactgga ttcactacaa tctggggttg ctcgagaagg aactcggacg
     6241 ccttgcagat gcagcaatct gctttcagat ggcgttacga atgaatccga agtttgagcc
     6301 cgcacagcag aaaatgaggg aaattgcggc tgccgcggct ggctaccccg tcgatggcaa
     6361 cagttcgatg aggaccgaac tgcctttccc cttcgaggtc aagcctccgg tgagcgttga
     6421 gggaaccccg cagacagaag atgcagagga tgaggatcag ggacccccgg tgcgacgctg
     6481 gggtggtacg aatcgtgggt cgagtgatgt cggcccggta tcgacggtgg ctgaatgtgt
     6541 ggcggttctc ggtcggcatc aacccgggcg tttcgggaaa accattgcgt tgaccttcga
     6601 tgatggcccg catccgtcct atacgaccca gttgctcgag tatctgcgat ctcagggggt
     6661 acgagcgacg ttctttgtgc ttggttcgcg ggcggaaacg tatccgcatc tcattacgcg
     6721 catgagcgat gaggggcacg aacttgccaa ccacacctgg agtcatcgtt ccctggtgaa
     6781 tcagagccag tctgcggcgc tctctgaaat gcgtcgtacg tctgagctgc tctccggtct
     6841 caccggtcgc tctccgcgtc tggtacgtcc tccgtatggt cacaccaatg cgaaagtggc
     6901 acggttgatt catgggcagg gatggcatca gatcatgtgg gatgcggaca gtcgcgattg
     6961 ggccggggga agttcgcaag ccatgctgca gcgggtgatg cggcagttct cgcccggtgg
     7021 cattgtgttg tttcacgaca tccacccggg aatgctgcga gttctgccgg ttttgattcc
     7081 ggcactgaag cagtgtggtt atcgctttgt cacggtgtcg gagttaatgg ctctcgatcc
     7141 agcgagctga agcagatgtc agccgctctc ttcgatttct gtctgattct gctgctgctg
     7201 ggatttttcc tgtacggact gcgtgagtgg gatcatctcc tggctgatcc ggaagaggac
     7261 cagcgggaaa aggctggtgg agccgcgtag ccgatgcctg tcggactcag catcgccctg
     7321 tgggttctct tcgccggtct gggcttggtc gctctcgcca cggatctgat gacctggtgg
     7381 cgcggcgagt gagccgtccg aagaacattt tcacagaacc ctaaaaactt cggcagctcc
     7441 tgccgatgaa agaagcggga aattcttccc cgtcaacagg aaagaatttc ccgcttcttt
     7501 catc
//
LOCUS       DAORHD010000050         7485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9538_length_7485_cov_3.984118, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000050 DAORHD010000000
VERSION     DAORHD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9538_length_7485_cov_3.984118"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..2805
                     /locus_tag="PKO06_01990"
     CDS             22..2805
                     /locus_tag="PKO06_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV68438.1"
                     /translation="MVRLRSLILGTIVVVLFAALIGCGNGNDAFPGEKSGGGTVRLSV
                     TAAGPAEMAPLAATRANGLVKPAILPSDIPTQQYRAIVKSAYGTFPVTSLYLSEDRRQ
                     IRFEETFRYAPEFTVEIYHQSYQPGGDWRSDLEHVLFTGVIAIPAMDNTQTVVQSASI
                     GAQETALGLSFAYWRQTTAPNAAFSQYLPQVESAQLTALAQRINEELRDLLPGQLAST
                     SFVFAWPEVVNDHAESLGQSIPAPPDAGDVTPPTVTAIVPVNNAANVTTGTTIRATFS
                     EGVLSSSLHAGTFSLQTAGGTPVTGTLSRDGVTVVFTPNAPLMFDTEYVLTIAGVQDQ
                     RGNVMNQPYSSGFRTISNPSSDLTPPTIVAVNPADGADGIATSTTITLTFSEAIDVTT
                     VTTTSIQLRQNGIPVTGSLFVTGSTQVTLTPAALAPATTCTLQVSGVHDLAGNALSQP
                     FTATFITRPADQAVAGAYRSYAFPVTGTNVSVYGQTATRPTSLVVVNPTSTSQSYVVS
                     GTLSTASIRASSSRRRATGGSVQRNLRQADFQGLLRELERRLPTFRRSSDQRPRPRIY
                     ADLVGNETTFSIVQDDGSTVTAPSTCRQIVTLPSPATSGNANFYFDQDIAWDATAQSF
                     VSQLAAAWPSIYATVRQKFGSEPPAGAFNGISLNDDITILIVSSEKIDPTGADLAGFF
                     YSGDLYSSAIDPASNQRKMLYLNYLPDSLSALKGVLAHELQHMVNFYQHKSRGLNEED
                     WLNEAMSGYAEHLCGFGVPTGNRSKALQTRDYLAAVSANSLTEWTGSHEDYGQVYLFG
                     TWLGQQYGTSGSVISLLAGTETGTDAVAAFTGKPFTDILTEWSIALLINDTTGGSRYG
                     IPGLSLRTTYPYNGGSVTFNGPQPVQRSAPFSETLGVAPHGCAVLEVTGGTGNRLQLT
                     VPTTGLFYELHAE"
     gene            2824..3774
                     /locus_tag="PKO06_01995"
     CDS             2824..3774
                     /locus_tag="PKO06_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV68439.1"
                     /translation="MIHRLGLTLSRLACHLPDTVGRRLAVGLGWTAAALLLHERRNLQ
                     ETIDRVYTRVGRPLPMPLSEIVNRVFPHFALNLYEILRYPLVTRDLLVRRFHVHGREH
                     FETALQRGKGVILVVPHLGNWETLGEYLAHYGYPLHSFYLAQKEDHLGSLLDHFRTYS
                     RIVLHDRDRGLVSALRTLKRGEVLGMISDQDGGNYGVYLDFLGHWVSLPAGPANWSLR
                     TGASVVRLYCLRRGLSADFDAWFLPALPAVEGTDHEACVQVRTKHMADWMQEIILRYP
                     HQYLWFYHRFKPRHEAQMSRLKQSGHRMRTGMTAYGIPPS"
     gene            3771..5081
                     /locus_tag="PKO06_02000"
     CDS             3771..5081
                     /locus_tag="PKO06_02000"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNV68440.1"
                     /translation="MIHPPNPSADELTAWGCTQALAGAVCLPLLALGPVLTRFSRRVR
                     HGIPHLLGQLPIPSCGPLIWLHGVSQGESMVAIGLGEHLKHRYPFCRLGFTTTHPEVL
                     ENARRKKHLDAVGFAPLDLLPFTMRAFRRWQPRLLIITETDFWPGLCALARRRGIPLA
                     LVNGRISEKLHRFHSRLLPLGRLIFRSFSLLAVQTETDRRRLIDMGAAPQAIQVLGNL
                     KADLAPTSAPDAVRVIGSWKGTSPLVIFGSLHPVEFEALRPEFAKLLRNHDCRLLIAP
                     RNTSLAREWQNSLSISGIPCCLRSQLTSTFAENERVHVIVLDTMGELGALYELGNLAF
                     IGGSLSLTVGGHNPIESIRYRVPTLVGPHTRNFADLIGDLTAADAIGIGHTATEIAGA
                     IDRFLQHPEDAVKMAVRASEVLATHQGAIARTLQALQPFLPHDH"
     gene            5166..6404
                     /locus_tag="PKO06_02005"
     CDS             5166..6404
                     /locus_tag="PKO06_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV68441.1"
                     /translation="MKKCPFCAEEIQDEARKCKHCGEFLDSGGLDRSLKAGDTVQQYH
                     VLRELGRGGMAVVFLADDTGLQKKVAIKALPPNLTHEPELTRRFLQEARLAAALTHPG
                     IITIHTVGATPQGQPFFVMEYLPGDRLSGKLQQGPLPIPEATRIMRETLAALGYAHAR
                     KVIHRDIKPDNILFRDDHSVVIADFGIAKATMSSQAMTATGMIMGTPHYMSPEQCRGD
                     TVDHRSDLYSCGIVLFHMLTGKLPFTGDGMHALMYAHLRQAPPIPSHLNAQVPKALDA
                     VVLKALAKNPDERFANADAFLQALKDDARVPADPLYEPATQRLTPLSEDFAASGEPVS
                     PSSPPSPSSAAPIQSSREKPVTEDSRAVSEGAPIPSPMPAAQRENPTAALAPTIGAVL
                     MTLLIISYSFLYYLGSGESF"
     gene            6504..7163
                     /locus_tag="PKO06_02010"
     CDS             6504..7163
                     /locus_tag="PKO06_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68442.1"
                     /translation="MRSLFLTLVYLGFTVPVLLRVGDSEQILILTAIMATLGASMAIL
                     ARAIFQLRSDPDDWAFLTSCRQRPSNTLSDHDRRKCDYISNHAFCSLYFSGITVCFIF
                     WRILCEESNPLDVFPSSELAPLVPALIPVLLFLLLEEWLSYTLTASDADAADPLAFWR
                     LAVQVLVMAYAALGPLQNETVSDDARMLLIICGAGIPPLIFTISFLYRLMWREQASGE
                     A"
     gene            7163..>7485
                     /locus_tag="PKO06_02015"
     CDS             7163..>7485
                     /locus_tag="PKO06_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68443.1"
                     /translation="MGGTDLLLISCSVLVIGGFLLSWSRYRFAGAVIQTLGFTVISAA
                     LVISLTDFIRMQVRTTTFLLLQCLWLAGGTGSLLLMRLNRHSLETLVQAFWPGSPPTT
                     PEELAR"
BASE COUNT         1391 a         2390 c         2132 g         1572 t
ORIGIN      
        1 gacttgcggc gaggtcgtca tatggtacgt ctgcgttcac tgattctcgg tacgatcgtt
       61 gtggtgttgt ttgcagcact gatcgggtgc gggaatggga atgacgcgtt tccgggcgag
      121 aagtcgggcg gcggcaccgt tcgactttcg gtcacggctg ccggccctgc cgagatggcc
      181 ccgctggcgg caacgcgcgc caacggtctg gtgaagccgg cgattctgcc ctcggacatt
      241 ccgacccagc agtatcgggc aatcgtcaaa agcgcgtacg gaacgtttcc cgttacgtcg
      301 ctctatcttt ccgaagaccg gcgccagatt cggtttgagg aaacctttcg ctatgcgccc
      361 gagttcacgg tcgagatcta tcaccagtcc taccaacccg gtggcgattg gcggtctgat
      421 ctcgaacacg tgcttttcac cggtgtcatc gccattccgg cgatggacaa cacgcagacc
      481 gtcgtgcaat cagccagtat cggcgcacag gagacggcac tcggcctttc gtttgcctac
      541 tggcgccaga cgaccgcccc gaacgccgca ttctcgcagt atctgccgca ggtcgaaagc
      601 gctcagctca cggctctggc ccagcgcatc aacgaggaac tgagagacct gcttcccggg
      661 caactcgcga gcacctcgtt cgttttcgcc tggcccgagg tcgtgaacga ccatgcggaa
      721 tcgctcggac aaagcattcc cgctccacca gacgcgggcg acgtgacacc tccgaccgtg
      781 acggccatcg tcccagtgaa caacgcggcg aatgtcacga ccggcaccac cattcgggcg
      841 acgttcagcg aaggcgttct ctcctcatcg ctgcatgccg gtactttcag tctgcaaacg
      901 gctgggggca ccccggtcac cgggactctg agccgcgatg gagtcaccgt cgtcttcacg
      961 ccgaatgcgc ccctgatgtt cgataccgag tatgtgctca cgattgccgg cgtgcaggat
     1021 caacgcggca atgtcatgaa tcaaccgtat tcaagcgggt ttcgcacgat cagcaacccg
     1081 agcagcgatc tgacgccgcc caccatcgtc gcggtgaatc ccgccgacgg cgcagacggc
     1141 atcgccacgt cgacaacgat cacgctgacg ttcagtgaag cgatcgatgt gacaacggtc
     1201 accacgacca gcattcagct gcgccagaac ggcataccgg tcacgggaag tctcttcgtg
     1261 accggctcga cccaggtgac gctcaccccc gcggcgctgg cacccgcgac cacctgcacg
     1321 cttcaggtca gcggggttca cgacctggcc gggaatgcgc tgagtcaacc attcaccgcc
     1381 accttcatca cccgcccggc ggatcaggcc gtcgccgggg cgtatcgttc gtacgctttt
     1441 cccgtgacag gaaccaatgt gtcggtctac ggacagaccg cgacacgccc cacgtctctg
     1501 gtggttgtca acccgacctc gaccagccag tcctacgtcg tctcgggaac gctgtcaacc
     1561 gcatccatca gggcctcttc gtcgcgacgg agagcaaccg gcgggtctgt tcagcggaac
     1621 ctgcgacaag cggactttca ggggctgctc cgcgaacttg aacgtcgatt gcccactttt
     1681 cgtcgcagtt ccgaccagcg tccgcgaccc cggatctacg ccgatctcgt cggcaacgag
     1741 acgacgtttt ccatcgtcca ggacgacgga tcgacggtga cggcaccgtc gacctgccgt
     1801 cagatcgtga ctttgccgtc accggcgacc agcggaaacg ccaacttcta tttcgaccag
     1861 gacatcgcct gggatgccac ggctcaatcc ttcgtcagtc aactggcggc ggcgtggccg
     1921 tcgatctacg cgacggtccg gcagaagttc gggtcggagc cgcctgccgg cgcgttcaac
     1981 ggaatcagcc tgaatgacga catcacgatt ctcatcgtgt cttcagagaa gatcgatcct
     2041 actggcgcag atctggccgg ttttttctac tctggagact tgtactccag cgccatcgac
     2101 ccggcgagca atcagcgcaa gatgttgtat ctcaactacc tgcccgattc gctgagcgcc
     2161 ttgaagggag tcctcgcgca cgagttgcag cacatggtca atttctacca gcataaaagt
     2221 cgcggactga acgaggagga ctggctgaac gaagcgatgt ccggatacgc ggagcatctg
     2281 tgcggttttg gagtaccaac cggaaaccgg tcaaaagcac tgcagacgag agattacctg
     2341 gctgccgttt ccgcaaactc gttgacggaa tggacgggaa gtcatgagga ctacggtcag
     2401 gtgtatctgt tcggaacctg gcttggtcag cagtatggga catcgggttc ggtcatctct
     2461 ctgcttgccg gcactgaaac cggcactgac gctgtcgccg cgttcaccgg caaaccattc
     2521 accgacattc tcaccgagtg gtcaatcgcc ctgttgatca atgatacaac cggtggcagc
     2581 cggtatggaa ttccgggctt gagtctacgc accacttacc cctacaatgg cggatcggtc
     2641 accttcaatg ggccgcaacc ggttcagcgc tccgccccgt tcagcgaaac cctgggggtc
     2701 gcgccacacg gttgtgccgt tctcgaggtg accggaggca cggggaaccg attacagctc
     2761 acggttccga ccaccggtct tttctatgag ctgcatgcgg agtaacgttc acacacaccg
     2821 atcatgattc atcgcctggg actcacgctt tcacgcctcg cctgtcatct gccggacacg
     2881 gtggggcgac gtctggccgt cgggctgggt tggaccgcgg ccgctctgct gctgcatgag
     2941 cgccggaatc tgcaagagac catcgaccgc gtctacacga gagtcggtcg accgcttccc
     3001 atgcctctct ccgagatcgt gaaccgggtg tttccacact tcgccctgaa cctgtatgag
     3061 atcctgcgat atccgctcgt gacccgcgat ctgctggtgc gtcggtttca cgtgcatggg
     3121 cgcgagcatt tcgagacggc cctgcagcgc ggaaaagggg tgatcctggt cgtgccgcat
     3181 ctgggcaact gggagaccct gggggagtat ctcgcgcatt acgggtatcc gctgcattcg
     3241 ttctacctgg ctcagaaaga agaccacctc ggcagcctgc tcgatcactt tcgcacgtat
     3301 tcacggatcg tgctgcacga tcgagaccgt ggtctggtca gcgctctgcg caccctgaag
     3361 cggggcgagg tgctcggcat gatctcagac caggatggcg gcaattacgg ggtctacctc
     3421 gactttctcg ggcactgggt cagtctgcca gccggacccg ccaactggag cctcagaacg
     3481 ggagcgagcg tcgttcgcct ctactgcctg cggcgcggtc tgtcggctga ctttgatgcc
     3541 tggtttctgc ctgcactgcc ggcggtcgag ggaactgatc acgaggcatg cgtacaggtt
     3601 cgaaccaagc atatggcaga ctggatgcag gaaatcattc ttcgttatcc gcatcagtat
     3661 ctctggtttt accatcgctt caaaccccgg catgaggcgc agatgtccag gttaaagcaa
     3721 agcggacatc gcatgcgaac cggcatgacc gcatacggaa ttcctccgtc atgatccacc
     3781 caccgaaccc ttccgcggac gaactgacgg catgggggtg cacgcaggcg ctcgcaggcg
     3841 ctgtctgtct gccattgctg gcactcggtc cggtgctgac acgtttctca cgacgagtca
     3901 ggcacggaat accgcacctg ctggggcagc tgccgattcc cagctgtggg cctctgatct
     3961 ggctgcacgg agtttcgcag ggcgagtcga tggttgccat cggcctgggc gaacatctca
     4021 aacaccggta tccgttctgc cgcctcgggt tcacgacgac ccatccggag gttctggaaa
     4081 acgcccgccg caagaagcac ctggatgcgg tcggctttgc cccgctcgat ctgctgccgt
     4141 tcacgatgcg ggcgtttcgc cgctggcagc cgcgacttct catcatcacc gagaccgatt
     4201 tctggcccgg tctctgtgcg cttgcccgcc ggcgcggcat tcctctcgcg ctggtcaacg
     4261 gccgcatcag tgaaaagctg catcgattcc actcacggct gttgcctctc gggcgcctca
     4321 tctttcgctc gttttctctg ctcgccgtgc agacggagac cgaccgtcgc cgcctgatcg
     4381 acatgggagc agccccccag gcaattcagg ttctcggcaa cctgaaagcc gatctggccc
     4441 caacgtcggc tccggacgcg gttcgggtca tcggctcctg gaaggggaca tcgcctctcg
     4501 tgatcttcgg atctctgcac ccggtcgagt tcgaggcgct gcgcccggag ttcgccaagc
     4561 tgctgcggaa ccacgattgt cgtctcctga ttgctccgcg caacaccagc ctggctcgcg
     4621 agtggcagaa ctcactttcg atttctggta ttccatgctg tttacgtagt cagctgacca
     4681 gtacttttgc cgaaaacgag cgggtgcacg tcatcgtgct cgacaccatg ggcgaactgg
     4741 gcgcgctcta tgaactcggc aacctggcct tcatcggcgg ctctctgtcg ctgaccgtcg
     4801 gggggcataa tcccatcgaa tcgatccggt atcgcgtgcc cactctggtc ggcccacata
     4861 cgcgcaactt tgcggatctc attggagatc tgaccgctgc tgacgccatc ggcatcggtc
     4921 atacggccac cgagattgcg ggggccatcg atcgctttct ccagcatccg gaagacgccg
     4981 tgaagatggc ggtacgagca tcggaagttc tggccacgca tcagggggcc attgcacgca
     5041 ccctgcaggc attgcagccg ttcctgccgc atgatcacta agcgaaccgt gaaatcgttt
     5101 tctctcttcg cggcttgtgg tagggtgatg ggacaaacgc ggaaagaggt gaggcagtca
     5161 gcggcatgaa gaagtgccca ttctgcgcgg aggaaatcca ggacgaagcc cgcaagtgta
     5221 aacactgcgg cgagtttctc gattcgggtg gtctcgaccg ctcgctcaaa gccggggaca
     5281 ccgtgcagca gtatcatgtt ctgcgcgaac tcgggcgcgg cggcatggcc gtcgtctttc
     5341 tcgccgatga caccgggttg cagaagaaag tcgccatcaa agcgctcccc cccaatctga
     5401 cccacgaacc ggaactcacc cggcgttttc tgcaggaagc tcgtctggcc gctgcattga
     5461 cacacccggg aatcatcacg attcacaccg tcggtgccac gccacagggg caaccgtttt
     5521 tcgtcatgga gtatctgccc ggagatcggc tttcgggaaa actgcagcaa ggccctctac
     5581 cgattcccga agccacgcgc atcatgcggg aaacgctggc agcccttggc tacgcgcatg
     5641 cccgcaaggt cattcaccgc gacatcaagc cggataacat tctctttcgc gatgaccact
     5701 cggtcgttat cgccgacttc ggcattgcca aggccacgat gtcctcgcag gccatgaccg
     5761 cgacaggcat gatcatgggc acgcctcact atatgagccc cgaacaatgc cgtggagaca
     5821 cggtcgatca tcgaagcgac ttgtactctt gcggcatcgt actgtttcac atgctcaccg
     5881 gcaaacttcc gttcaccggg gacggcatgc atgcattgat gtatgcccat ctgcggcagg
     5941 cgccccccat tccctcgcac ctgaacgctc aggtcccgaa ggcgctcgat gcggtggtcc
     6001 tgaaagcgct ggccaagaac cccgacgagc gttttgccaa tgcggacgcg tttctgcagg
     6061 ccctcaagga tgacgcccgg gtgccggcag acccgctcta cgaaccggca acccagcgac
     6121 tgactccgct ttcagaagat ttcgccgcca gcggcgagcc ggtctctcca tcgtctcccc
     6181 cgtctccatc gtctgccgcc ccgatccagt cgagccgtga aaaaccggtc actgaagaca
     6241 gccgcgctgt ttccgaagga gcgcccattc cgtccccgat gccggccgcg cagcgtgaga
     6301 acccgactgc cgctctggca cccacgatcg gcgccgttct gatgactcta ctgatcatca
     6361 gttacagctt tttgtactac ctcggttccg gagaatcctt ttaacatggg cgtcacgcgg
     6421 agtagaaaga gcgttttcag gcaacgggca tttcctcggg ggagtattcc cgatctccat
     6481 tccggtcggc gaagaggtgg ttcatgcgtt ctctctttct gacgctcgtc tatctggggt
     6541 tcaccgttcc cgtgctgctc cgcgtcggtg actcggaaca gatactcatt ctgaccgcca
     6601 tcatggcgac tctcggcgcc agcatggcga ttctggcccg tgccatcttt caactgcgtt
     6661 ccgaccccga cgactgggct ttcctcacat cgtgccgtca gcgaccgagc aacaccttgt
     6721 ccgaccacga tcgccgcaaa tgtgactaca tcagtaatca tgccttctgc agcttgtact
     6781 tcagcggcat cacggtttgt ttcatcttct ggcgcatcct gtgtgaagag tcgaaccctc
     6841 tcgatgtatt tccgagctct gagctggcgc ctctggtgcc ggcactgatt cccgtccttc
     6901 tctttctgct gctcgaggaa tggctttctt acaccctgac cgcgagcgac gcggatgccg
     6961 ccgacccgct ggcattctgg cgtctggcag tacaggttct ggtaatggct tatgccgcac
     7021 tcggtccgtt gcagaatgaa acggtatcgg acgatgcgcg catgctgctc atcatctgcg
     7081 gggcaggcat tcccccattg atcttcacca tctcgtttct gtaccggctc atgtggcgag
     7141 aacaagcttc aggagaagcc tgatgggtgg aactgatctg cttctgattt cctgcagtgt
     7201 tctggtaatc ggcggctttc ttctcagctg gtcgcgatat cgttttgcgg gagccgtgat
     7261 ccagacactt ggttttaccg tgatcagtgc tgcgctggtt atttcgctca cggacttcat
     7321 tcgcatgcag gtccgtacca ccacgtttct tctgctgcag tgtctctggc tggctggcgg
     7381 caccggatcg ctgctgctga tgcgtctgaa ccgacactct ctcgagaccc tggttcaggc
     7441 gttctggccc ggttcaccac cgacaactcc cgaagaactg gcccg
//
LOCUS       DAORHD010000051         7451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9600_length_7451_cov_3.507031, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000051 DAORHD010000000
VERSION     DAORHD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9600_length_7451_cov_3.507031"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PKO06_02020"
     CDS             complement(<1..849)
                     /locus_tag="PKO06_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68444.1"
                     /translation="MTEPVKEASAIPTASPSGRLLFSSKPFTFELFQLWFVTLAFGLV
                     FLAANRLGLGAADPNRVRLVFSTFVIINIAGAFMAFQGWKQIPTRGRVYDLALFLSMA
                     LLCAAIANSLDLILWAAGAYPIKTSVFPNIFFVFALVFGLLGIFKLAQLCLVTPGLLT
                     AAIFSLLVALFLLIPYKFNSTLFGSIPNLENQKEFLFGMLYSFVIAFIGSVCAQIMIS
                     SRGFFRLPAQFICVGTIGLSFGCSIYGAQFLILDTMSVSASPVHVILGLSYVSIGLGV
                     YRLGNVV"
     gene            complement(1050..7064)
                     /locus_tag="PKO06_02025"
     CDS             complement(1050..7064)
                     /locus_tag="PKO06_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV68445.1"
                     /translation="MSADSSTRRWLIPAFIVGLSCFLLVLYAVGCRIIDPGYGNGYVP
                     EGPTITLFQSTIQSNQEYRIQGEGFELLIPTGAMPRAMQLSVFRTLRETNIFPVPASW
                     TVLSNVYTISHMGGIGSYNLGQLYRPAALRIPFATDLVPTGADQSDIFIACYDAAQGL
                     ILRTPSIDMTNGMAMIETYHFSMWAVVARPVDLRPKAFPNAPLVETDPTIMAATTATR
                     YAKDLAFTINFELPSGAATTGNLSGWYLQFFPAPATGLVLGHPTINGSTRPLTAGFPF
                     LEPIDLLKDPLVTRTYDGSRGTFAFRLIFTDKTPADAPATLIWRLGARDPLGILYERE
                     GHVTFTVENRQIVLTAPFGSIAPATSIPFTWNLLPGVGAYESVLLQIADHPEGFLLPR
                     FETLLKPATTTYTLDADATLLLPSDKQLYWRIVGYDLYKNQVVTRPATFRIGLTPDAL
                     TVLNTLPASGALGVSPATSISVVFSRAVASSTILPSLSITPQSVGDLQLSANSFSYTP
                     ETPLLENTRYIVGLATTVTDLNGHSLEAPFEFSFTTGTLGAAGFANLLQFVPALGQSE
                     VPPRQSLELAFDRDMVADSVRTSFSLTPPVPGAAPIWTWLTPRHARVSFPDGLAYATT
                     YQARLAATAASLGGQTLQQDVRWEFRTAAQDDVTAPLLVSITPAGGARNVPLNALVTF
                     TFSEPMDPAAFEAALSVLNPAVGALEFHWVPGSEIVSVKPVAGWKPQAAHTLRIGASA
                     RDVSGNQLPEAVETSFTTSNETGPIIVSSRPANQELTAQPVTRLEFTFDRPMDREKVQ
                     AAYSVIPLPVAAPTFLWSENDTRLAVSYPLAFPERTNVTAAFSALAQDLNGQSLAGQV
                     SLTFRTGDFTSPTLVSIVPASGSTLIPQNAQLFVRFSEPMQTSSPEKVVTLSPPLPGV
                     TYSWSPAATELTLERGTPWPIAAQSFTLSGAMKDAAGNQLGASTTFTLSFTSSTAPQV
                     ALSVPANGAANVSRTAPIMLTFDQPMNQAVTAGACQITPSVSFTPSWNGDSTILTLTP
                     SISLANATVYQLTLSTAATGQNGIPLVSPVTVTFSTADTIAPELLAVTPAHQAFGVSR
                     TAALTFTFSEPVNTAAAQVAYSLNPVGPSAPTFSWSNANRTVTVSYSAPFPEETRYRA
                     RFSTLLSDAAGNTPTAAFESEFTTESTVIPEVAEFQPASGASGVAMNTALRFAFNKPM
                     ATSTVLVTLSPDPGGTPLYSWSNDAKTLTYTPATQLTAGQNYLTTLSETARDLFQNPL
                     SAPRSISFTVGNSLAAQTSCNPTNGATGLILTTQPVLTFTKAMNTASVMADLTIDPPG
                     PRTPTWSNGNKTLTLDLGALVASTSYTLSLAPTIVDAEGVPLGQTFTSTFVTRPPAPT
                     VVSVNPENGATGIGLSSPITITFSQPMNQGTVNISAAPSDPLGTRTITWSANGQVMTV
                     SWSTGFEANKAYTITLTGNDTYGQALTGDVDSQFTSATVSGPTIVSFSPTIGTQGASR
                     TVRLTALFSRAMKPDTTQAAFSLTPPPTGVPAFAWSGDAKTLDITFSQPLDYDTYYTA
                     AFSTTACDPNLVRLNTAYSSWFVTETRPAILAANVSPSAGATEIASGAVIRVPFSKPM
                     NLAVTQAAFSVKIGGVAIPGAFSWDSNTLIFTPASPLPAATTCTVAVTTAAADTTGNH
                     LASPWSSTFVTYTPDLASWRKDLDFEDEGSGTQFPQRTGHALVTFDNKLWVIGGTDGL
                     NDLNDVWWSANGVNWTRATAAAAFSARHGHACTAWNNAIWLTGGVNQVIVDGFPEDNY
                     LDDVWRSTDGITWTRMSASAEYYSRAEHVLLVFNNQLWMVGGITTDTDGNVVLLGDAW
                     NSTNGSNWTERSQVVGFTPRRGAAAYSALNRLWVVGGFGVDSVTQQQKSLNDVWSSVN
                     GDTWTRVTATTPLTGRSGAGFAFFKNRLTLVGGKGVDAGGWDILLNDVWTSADGLNWN
                     RNRADGIAADHFSRRSGHSLSLLGTRLYLVGGETDLGPFNDVWSAP"
     gene            complement(7061..>7451)
                     /locus_tag="PKO06_02030"
     CDS             complement(7061..>7451)
                     /locus_tag="PKO06_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68446.1"
                     /translation="ERPSKPPVAQPAVTATASTRHVVGSCPLCGEGRIVKGNSAYGCL
                     RFREGCQFRLPFEYLGKTLSEAMVKQLLNGKETRASVGFTSPDGTKQSGKLRLNKTTR
                     EIELVPIDQTSSTGTTRSRKKKEKEET"
BASE COUNT         1595 a         1916 c         2466 g         1474 t
ORIGIN      
        1 aacgacgttg cccaggcggt agaccccgag accgattgag acgtaggaga ggccgagaat
       61 cacgtgcacc ggactggccg acacgctcat cgtgtcgaga atcagaaatt gagctccgta
      121 gatcgaacag ccgaaactca atccgattgt tccgacgcag atgaattgag ccggcagtct
      181 gaaaaagccg cgcgatgaaa tcatgatctg cgcgcagacc gagccgatga aggcgataac
      241 gaacgaatac agcattccga acaaaaactc tttttggttc tctaagttag gaatagagcc
      301 aaaaagagtt gaattgaact tgtacggtat gaggagaaac aaggcgacca gcagcgagaa
      361 gatcgcggcg gtcaataacc ccggagtgac cagacagagc tgcgccagtt tgaaaattcc
      421 cagcaggccg aaaacgagag cgaagacaaa gaaaatgttc ggaaataccg acgttttgat
      481 cgggtaggcg cccgccgccc agagaatcag gtcgaggctg ttggcgatgg ctgcgcagag
      541 caacgccatg ctgagaaata aggcgaggtc atacacgcgt cctcgggttg gaatctgctt
      601 ccaaccctga aatgccatga aagcaccggc aatgttgatg atgacgaagg tcgaaaacac
      661 cagccgcacc cggttgggat cagcggctcc gagtccaagg cggttggcag ccagaaagac
      721 gagaccgaat gccagggtga cgaaccacag ttggaacagt tcgaaggtga agggttttga
      781 cgaaaacaac agccggccgc tgggcgatgc ggtgggaatg gctgaagctt ccttgacggg
      841 ttcggtcata gcttggtcgt gaaagacact ctctcagaat tcattcgttc agtctcattc
      901 taccgtacgc tgtcgtgctg gtctatcaaa gcgtgaggga tcatgaattc tgcctgagat
      961 caggaggaat acggccagct cctttttctg atacaggccg ttgtcgaaaa caaaagcggt
     1021 agggtgaagg gattccagcc atgagcgggt tatggtgccg accagacatc gttgaacggt
     1081 ccgagatcgg tttcgccacc cacgagatac aggcgggtgc ccaggaggct gagagaatgg
     1141 ccggatcggc gcgaaaagtg gtcagcggcg ataccgtctg cgcgattgcg gttccagttg
     1201 agaccgtcag cactcgtcca gacgtcattc agcagtatgt cccagccgcc tgcgtccact
     1261 cctttcccgc cgacgagcgt gagacggttc ttgaagaagg cgaacccggc accacttcgc
     1321 ccggtcagcg gagttgttgc cgtcacgcgt gtccacgtgt ctccgttcac gctgctccag
     1381 acatcgttca gcgatttctg ctgctgcgtg accgagtcga cgccgaaccc gccgacgacc
     1441 cagagacgat tcagagcgga ataggccgca gcgccgcgtc gtggggtaaa accgaccacc
     1501 tgactgcgtt ctgtccagtt tgagccattc gttgaattcc aggcatcccc gagcagcacg
     1561 acgttgccat ccgtgtcggt ggtgatgccg ccgaccatcc acagctgatt gttgaagacg
     1621 agcagaacat gctcggcacg cgaatagtat tctgctgacg cggacatcct ggtccaggtg
     1681 atgccgtcgg tcgaacgcca cacatcgtcg agatagttgt cctcggggaa tccgtcgacg
     1741 atgacctggt tgacaccgcc ggttaaccag atcgcgttgt tccaggccgt gcaggcgtgc
     1801 ccatggcgag ccgagaaagc agctgcggcc gtggcgcgag tccagtttac gccgttggct
     1861 gaccaccaga cgtcattcag atcgttcagc ccgtcggtgc cgccgatgac ccagagtttg
     1921 ttgtcaaacg tgaccagggc gtgaccggtg cgttgcggaa actgtgtgcc gctgccttca
     1981 tcctcaaagt cgagatcttt gcgccacgag gcgaggtcgg gggtataggt gacgaacgtg
     2041 ctgctccagg gcgaagccag gtgattgccg gtcgtgtcgg ctgcggctgt cgtgacggct
     2101 acagtacaag tggttgcggc tggaagtggg cttgcaggag tgaaaatcag agtattggaa
     2161 tcccaggaaa aagcgcctgg aatcgcgacc ccgccgatct tcacgctgaa agccgcctgc
     2221 gtcacggcca gattcattgg cttcgagaac ggaacacgaa taacggctcc agaggcgatt
     2281 tcggttgctc cggcactcgg actgacattc gccgcgagaa tggcgggccg ggtttccgtc
     2341 acgaaccacg aggaataggc ggtgttcaaa cgaacgagat tcggatcgca ggcggtggtg
     2401 ctgaaagcgg cggtatagta ggtgtcatag tcaagcggtt gcgagaaggt gatgtcgagg
     2461 gtcttggcat caccggacca cgcgaacgcc ggaaccccgg ttggcggtgg ggtcagcgaa
     2521 aaagccgcct gggtggtgtc cggcttcatg gctcgactga aaagcgccgt caaacgaact
     2581 gttcgtgatg ccccttgagt gccgatggtt ggtgagaaac tgacaatggt tggcccggaa
     2641 acagtcgcgg aggtgaactg cgaatcgacg tcaccggtca gggcctgacc atacgtgtcg
     2701 tttccggtga gcgtgatggt gtaggctttg ttcgcctcga agccagtcga ccagctgacc
     2761 gtcataacct gtccattcgc ggaccaggtg attgtccgag ttccaagcgg gtcagatggc
     2821 gcggcagaga tgtttaccgt gccttgattc atgggttgag agaatgtgat cgtgatcggt
     2881 gatgagagcc cgattccggt ggctccgttc tcggggttca ccgacacgac tgtgggtgcg
     2941 ggtgggcggg tgacaaacgt gctggtaaac gtctgtccca atggcacgcc ttcggcatcg
     3001 acaatcgttg gggcaagcga aagggtgtag ctcgtcgatg ccaccagagc acccaggtca
     3061 agggtaaggg ttttgttccc gttgctccag gtcggcgtgc gagggccggg ggggtcgatt
     3121 gtcaggtcag ccatgacaga ggccgtgttc atggccttgg taaacgtcag tactggttga
     3181 gttgtcagaa tcaagccggt cgcgccgttg gtcggattac aagacgtttg agccgccaga
     3241 ctgttgccca cggtgaaaga gatggaacga ggggcggaca gcgggttctg aaacaagtcg
     3301 cgcgcggttt cggaaagagt tgtcagataa ttctgtccgg ccgtcagttg cgtcgcgggg
     3361 gtgtatgtca gggtctttgc atcattcgac cagctgtaca atggcgttcc gccaggatct
     3421 ggcgacagag tcaccagtac ggtagaggtc gccatcggct tgttgaatgc aaagcgcagc
     3481 gcggtgttca tggcgacgcc actcgcgcct gaagcgggtt ggaactcggc cacctcggga
     3541 atcaccgtgg attccgtggt gaactcgctc tcgaaggcgg ctgtcggagt gttccccgcg
     3601 gcatcgctca gaagagtcga gaatcgagct cgatagcggg tttcttccgg aaagggtgcg
     3661 ctgtagctca ccgttactgt gcggttggca ttcgaccacg agaaggtggg agcgcttgga
     3721 ccgacggggt tcaggctata ggccacctgg gccgccgcgg tgttgaccgg ttctgagaac
     3781 gtaaacgtga gtgcggccgt gcgcgataca ccgaatgcct gatgtgcagg agtgacggcc
     3841 aacagctcag gagcgatggt atccgcggtg gagaaagtca cggttaccgg cgaaaccagg
     3901 ggaatgccat tctgaccagt ggcagccgtc gaaagagtca gctggtacac ggttgcattg
     3961 gcgagtgaga tggagggagt cagcgtcagg atcgtggaat cgccgttcca ggatggggtg
     4021 aatgagacgg aaggagtgat ctgacaggct ccggctgtaa cagcctgatt catgggctgg
     4081 tcgaaggtga gcatgatcgg ggcggtgcgg ctgacgttgg cagcgccgtt tgccggaacc
     4141 gacaaggcta cctgcggggc cgtgcttgaa gtgaaggaga gcgtgaatgt ggtggaggct
     4201 ccgagctggt ttccggcggc gtctttcatg gccccggaga gggtgaagct ctgcgcggcg
     4261 atcggccagg gcgtgccacg ttcgagggtg agttcggtgg cagctggcga ccaggaatag
     4321 gtgactccag gcaagggtgg agaaagtgtt accacttttt ccgggctgga cgtttgcata
     4381 ggttcagaaa atcggacaaa gagctgcgca ttctggggaa tcagcgtgct gcccgaggcc
     4441 ggcacgatcg aaaccagggt gggcgaagtg aagtcgccgg ttctgaacgt cagagaaacc
     4501 tggccggcca gagactgtcc gttgagatct tgtgccagcg cgctgaatgc cgcggtcaca
     4561 ttcgtacgtt caggaaaggc caacggatag gagaccgcaa gtcgggtatc gttttcgctc
     4621 cagaggaacg tgggcgcagc cacgggaaga gggataacgg aataggcggc ctggactttt
     4681 tcacggtcca tcggtcgatc gaaggtgaac tcgaggcgcg tgaccggttg tgcggtcagt
     4741 tcctgattcg ccggtcgcga agacacgatg atcgggcccg tctcgttgct ggtcgtgaaa
     4801 ctggtttcaa cggcctcggg caattggttg ccgctgacat cccgtgcgga ggctccgatg
     4861 cgcaacgtgt gcgccgcctg cggtttccag ccggcgactg gtttgaccga gacgatttca
     4921 ctgccgggaa cccagtgaaa ttcgagtgcg ccgaccgcgg ggttgagaac cgagagagcc
     4981 gcttcaaacg cggctggatc catcggctcc gagaaggtga aggtgaccag cgcattgagc
     5041 ggcacattgc gagcaccacc ggcgggcgtg atactgacaa gcaacggcgc ggtcacgtca
     5101 tcctgggccg cggtccgaaa ctcccatcgc acgtcctgtt gcagcgtctg accgccgagg
     5161 ctggctgcag ttgcggccaa gcgagcctga tacgtcgtgg cgtaggcgag tccgtccggg
     5221 aaagagactc tggcatgtcg gggcgtcagc caagtccaga tgggagcggc gccaggtacc
     5281 ggaggcgtga gagagaaact ggtacgcacg gagtcggcga ccatgtcgcg gtcgaaggcc
     5341 agttcgagcg attgacgcgg aggaacctcg gactgcccca gggcgggaac gaactgcagg
     5401 agattggcga agccggcggc tccgagtgtt ccggtggtga aggaaaactc gaagggggct
     5461 tccagagaat gcccgttgag atcagtgact gtggtggcaa gaccgacaat gtagcgcgtg
     5521 ttttccagca gaggcgtctc gggagtgtac gagaacgaat tcgcggaaag ctgcagatcc
     5581 ccgacactct gaggtgtgat cgacagcgag ggcagaatcg tgctcgaagc cacggcgcga
     5641 ctgaacacga cgctgatcga ggtggcggga gaaacgccca gggccccgga agcgggcagg
     5701 gtgttcaaga ccgtcagtgc atccggagtc agaccgatgc gaaatgtcgc cggtcgggtg
     5761 actacctggt ttttgtacaa gtcatatccg acaattctcc agtataattg cttgtctgac
     5821 ggaagtagaa gtgttgcgtc agcgtcgagg gtgtaggtcg tggtggccgg tttgagcagg
     5881 gtctcgaatc gtggcagaag aaatccctcg ggatggtcgg caatctgcaa caacaccgac
     5941 tcgtacgctc caacgccggg cagcaggttc cacgtgaagg gaatcgaagt ggcgggagcg
     6001 atggagccaa acggagccgt gagcacgatc tggcggttct cgacggtgaa ggtgacgtgt
     6061 ccttctcgct catacagaat gccgagagga tctcgcgcgc cgagtcgcca gatcagggtg
     6121 gccggggcat cggccggcgt tttgtcggtg aagatcaggc gaaacgcgaa ggtgccgcgt
     6181 gatccgtcgt aggtgcgggt tacgagagga tctttcagca ggtcgatggg ttcgagaaag
     6241 ggaaaaccgg cggtcagggg tctcgtagat ccgttgatcg tcggatgacc gagaaccagt
     6301 cccgttgccg gtgccggaaa aaactgcagg taccagccac tcaggttgcc ggtggtggcg
     6361 gcgccgcttg ggagttcgaa gttgatcgtg aatgccaggt ctttggcata cctggttgct
     6421 gtcgttgccg ccatgatggt gggatcggtt tcgacgaggg gcgcgtttgg aaaagccttc
     6481 gggcgcagat ctactggtcg agccacgacg gcccacatgg aaaagtgata ggtttcgatc
     6541 atggccattc cgttggtcat atcgattgac ggcgtgcgca gaatcaaccc ctgtgcggca
     6601 tcatagcagg cgatgaagat gtcggattga tctgcgccgg tcggcaccag atcggtcgcg
     6661 aacggaattc gcaaagccgc cgggcgatag agttgcccga ggttgtacga gccgattcct
     6721 cccatgtgag aaatcgtgta gacgttgctg agcacggtcc agctggccgg taccggaaag
     6781 atgttggtct cgcgcagagt gcgaaagacg ctcaattgca tggcgcgcgg catcgctccg
     6841 gttggaatca acagttcgaa cccttctccc tgaatccggt attcctgatt tgactgaatg
     6901 gttgactgaa aaagagtgat ggtcgggcct tcaggaacat aaccgttgcc gtatccggga
     6961 tcgatgatgc ggcagcccac ggcatagagt accaggagaa aacaggataa acccacaatg
     7021 aacgccggaa tgagccagcg gcgagtggaa ctgtctgcgc tcatgtctcc tccttttctt
     7081 tcttcttgcg gctgcgggtc gttccggtcg atgatgtctg atcaatcggt accagttcga
     7141 tttctctcgt tgtcttgttg agacgcagtt tcccgctttg ctttgttccg tcgggtgatg
     7201 tgaatcccac gctggcgcgg gtctccttgc cattcagcaa ttgtttcacc atggcttctg
     7261 acagcgtttt gcccagatac tcgaagggga gtcggaactg acagccttcg cgaaaacgca
     7321 gacatccgta tgccgaattg cccttcacga tgcgcccttc accgcagagc ggacacgatc
     7381 caaccacgtg acgcgtcgag gcagtcgcgg ttaccgcggg ctgggccacg ggtggcttgg
     7441 atggccgttc a
//
LOCUS       DAORHD010000052         7348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9825_length_7348_cov_3.846291, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000052 DAORHD010000000
VERSION     DAORHD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9825_length_7348_cov_3.846291"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="PKO06_02035"
     CDS             <1..1201
                     /locus_tag="PKO06_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV68447.1"
                     /translation="TRDITERKRAAEERARLEEQIRQAQKLESLGVLAGGIAHDFNNL
                     LMAIMGNLELIGGCLPEDSSGQKYLTSAQKAAQRAAELCSQMLAYAGRGPMSRKLLQP
                     SRLLEELRPMLEMAVAKPARLVLDIPLELPPVEADQAQLQQALMNLVTNASEALLGQA
                     GEIRIRAWSELCGADRLQQGVLKEHLSEGPYVFLSVSDTGCGMDETLMPRVFDPFFTT
                     KFAGRGLGLPAVLGIVRSHRGTMTLQSQPGVGTTVTILLPVSEATDETAKSQERKRWK
                     GTGTILLVDDEASVREVTTLLLTRLGFRTRTAVEGAEALQLIELSEPSEFPVCVILDV
                     TMPGMDGIEAHRRIKARWPALPVIISSGYQAQDCLTHFHDPPPSGFLKKPYSLSQLEA
                     LLRSILR"
     gene            1251..2999
                     /locus_tag="PKO06_02040"
     CDS             1251..2999
                     /locus_tag="PKO06_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68448.1"
                     /translation="MLASAEAAKAVSALQYLVRKPELLTDEALRTAADRCLDHHDVAV
                     RFWARKLRSAVEQLAAKRIESSEGALTARSVSELLEMLKRAPSGYGAIEILRELVTRS
                     GDELRGTLLTYLAQTTDTVQISFLTKHLGLRFPDDDMLSRLLPYLKHGDDRVVANTVE
                     GVEPMESSAAVAVLAQLLSHPSQRVRANAAKALNRHAPEKATGVLRQMLQMHDQPNFV
                     LAACHAVRHLRHREFLPTLAGLLTDPVIASEAMKTISVICGDVAIQLLDQLPEVAKDV
                     IVRQRLLMAVEEHLAASCPVDQNPWAPLQASDNRRVIEVGEKRLFALQPSLTLDEAQK
                     KALANKRDAFGVISKFLYRPREDQIRLTYREMRYEPFWHVVCQLCLDYTRAREVDLNI
                     ERPVQSVRVGRQILTPEQGRIRLTMDEHCVEECLKEVFIDAQTGAPGPYQRHLAYPRR
                     EIRETEELMVDEVIVVPARIKASPVVRDLLTEILKPVRATEINHEQISLHRVNLLFRP
                     VYAFEFQWEDSQSFAVLEIDGLTGEVVSGKALKKKFDELFSESSLFELGSEAIDLVIP
                     GGGLAAKLARAVLGKN"
     gene            complement(3019..4650)
                     /locus_tag="PKO06_02045"
     CDS             complement(3019..4650)
                     /locus_tag="PKO06_02045"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNV68449.1"
                     /translation="MEADLLLVNGNVWRGGSQPEHQWTIAICGNRIAALGPDNQMKDW
                     RGPRTSVFDCRSRLVLPGFHDAHLHPLIGATDLLDCSLMGLKDWPSYQRHINTYLERH
                     PDASFVRGNGWVHGLFPAPGPHRQLLDSVIPDRPAYFKAMDGHSGWCNTAALRLAGIT
                     RQTPNPPGGIIERDPTTGEPTGLLRKMPAMQLVTDLIPQATHAELLNAADAFASQAAR
                     FGVTSVQDASGKSHFPAVYLELERSERLKTFRVTLVRRFSPGKGLGQLEESETLRHAH
                     HSPFFRFGPLKLFLDGVIEGETAYLLHDYSDRAGYRGEPVWDIAEWLEMIPRLAERDW
                     AVHVHAVGDAAVRLALDGIAGARAALPQAALRHQIAHADLISDADIPRFADLGVLANL
                     QPSWFARDSNYLGVTIPAIGNDRSEKLYRLRDLYNHRADVAFSSDWPFGGDVTSFDPL
                     IGIETGIRRIHPESHGDPLNPSQSATLTEMLTGFTLGAARAVNREHELGALRPGYLAD
                     LIVIEPDLFSVAPEEIHRSRVVLTVVDGSVIYRAD"
     gene            complement(4662..5423)
                     /locus_tag="PKO06_02050"
     CDS             complement(4662..5423)
                     /locus_tag="PKO06_02050"
                     /inference="COORDINATES: protein motif:HMM:NF020229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HNV68450.1"
                     /translation="MKLLLPVLLATVFFSLFEPQQINAEAPDQPTLKRRQISPLFIPP
                     ASPTLRIAFFDADSTLRIAPSGSPSANHATDVALLPLLTGPLQKLASEGYLIAIVSNQ
                     AGVAHGHVTLQNADAALAYTVERLASIGAPIHYYDFAELENEDRKPDIGMARRLADLA
                     STTWNKTIDWQNSLMVGDSAWKEGKDLEPDGTPGDDFSNSDRLFAENLQKTFGGTTFH
                     HPRDFFGWKAHGVRNFKSHQDLQNFIAKHPELAPR"
     gene            5723..>7348
                     /locus_tag="PKO06_02055"
     CDS             5723..>7348
                     /locus_tag="PKO06_02055"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNV68451.1"
                     /translation="MSRVTEMFGELTFNRDVMREKLSRDTYEKMLATLQTGNSLDESI
                     ADEVAHAMKEWAISKGATHFTHWFQPQRGGTAEKHDAFLTYAENGEIIERFSAKQLIQ
                     SEPDASSFPSGGIRSTFEARGYTAWDPTSPAFLMETGQTKTLVIPSVFLSWTGDVLDQ
                     KTPFLRSMRALNDAGIKLQRMLGNRMAKKVLVFTGPEQEYFVVSKKLYDTRPDLQICG
                     RSLFGAPPAKGQQMEDHYFGSIHRKMKKFMEDLDLELYRRGIPAKTRHNEVSPNQFEI
                     APLYEEANLAIDHNLQMMDIMKMVGQRHDLVVLLHEKPFAGVNGSGKHVNWSLGDNTG
                     ANYLEPSASPLKNINFLLTLGALLLGVDKFGGYLRATVADAGNDHRLGANEAPPAIMS
                     VYLGEYLSKLLAEIEAMGKFNEQSMAHINMGVRNLPRVVRDTSDRNRTSPIAFTGNKF
                     EFRAVGSSQNCSEAVTALNLLVTYGYYTIEKRLLAMSGKQDIKEKALVVLRDVFKETR
                     RVRFEGNGYSEEWHKEAAKRGLPNARNTPAALDA"
BASE COUNT         1578 a         1987 c         2271 g         1512 t
ORIGIN      
        1 gacgcgcgac atcaccgaac gcaaacgggc agctgaggaa cgcgcccgtc tcgaagaaca
       61 gatcagacag gcgcagaagc tcgagagtct cggcgttctc gccggaggca tcgcccacga
      121 tttcaacaac ctgctgatgg ccatcatggg aaacctggag ctgatcggag ggtgtctccc
      181 ggaagattcc agcggtcaga agtatctcac ctcggcccag aaagccgcac agcgggccgc
      241 cgaactgtgc agtcagatgc tcgcctatgc cggtcgtggc ccaatgagca gaaaactgct
      301 gcaaccttcg agactgctcg aagaactgcg gccaatgctc gaaatggcgg tggcgaagcc
      361 ggcccggctt gtactcgaca ttcctctgga attgccaccg gtcgaggcgg accaggctca
      421 gctgcagcaa gcgctgatga atctggtgac caatgcgtca gaagctctcc tgggccaggc
      481 gggcgagatc cgcattcgtg cctggtcgga attgtgtggg gctgatcgct tgcagcaggg
      541 ggttctgaaa gagcatctgt cggaagggcc gtatgtgttt ctgagtgttt ctgacaccgg
      601 ctgcggcatg gacgaaacct tgatgccgcg ggtttttgac ccgtttttca ccacgaagtt
      661 cgcaggacgt ggtctggggc tgccggcagt cctgggcatt gttcgcagcc atcggggcac
      721 gatgaccctg cagagtcagc ccggtgtggg tacgacggtc acgattctgt tacccgtgag
      781 cgaagcgaca gatgaaaccg caaagtctca ggagcgaaaa cgctggaaag gcacggggac
      841 cattctgttg gtcgatgacg aagcatcggt acgtgaagta acgacacttc tgctcacccg
      901 gctcgggttt cgcacgcgaa ctgccgttga aggagcagag gccctgcagc tgattgaact
      961 cagcgaaccg tccgagtttc cggtgtgcgt gattcttgat gtgaccatgc ctggcatgga
     1021 tggaattgag gcgcatcggc gcatcaaggc ccgctggccg gcgttgcccg tgatcatctc
     1081 gagcggctat caggcacagg attgtctgac acattttcac gatccgcccc cctccggctt
     1141 tctgaagaag ccgtattctc tctcgcaact ggaagccttg ctgcggagca ttcttcgcta
     1201 gtgcgaccac acgcatgaaa cccgtgaaca gtgtcgatag gatacgagag atgctggcct
     1261 ctgcagaggc ggcaaaggcc gtttcagcat tgcagtatct ggtacgcaag ccggaactgt
     1321 tgaccgatga agcacttcga accgccgctg accgctgtct ggaccatcac gatgtcgctg
     1381 tacgcttctg ggcgcgcaag ttgcgcagcg cggtcgagca gctcgccgcg aagcggatcg
     1441 agtcgagtga aggcgcactg accgctcgct cagtgtccga gttgctcgag atgttgaaaa
     1501 gagctccgtc agggtatggg gccatcgaaa ttttgcgtga gctcgtcaca cgctccggcg
     1561 acgagcttcg tggaacgctg ctgacctatc tcgcgcagac gaccgacacg gttcagattt
     1621 cctttcttac caagcatctt ggtctgcgct ttccggacga cgacatgctg agccggttgc
     1681 tgccatatct caaacacggc gatgatcgag tggtggccaa taccgtcgaa ggggtggagc
     1741 cgatggagtc gagcgccgcg gttgcggtgc tggcacaact gctgagtcat ccttcgcagc
     1801 gagtgcgagc caatgcggcg aaagcgctca atcgccatgc gccagagaag gcgacaggtg
     1861 tgttgcgaca gatgctgcag atgcacgatc agccgaactt cgtgctggcg gcctgtcatg
     1921 ccgtgcgaca cctgcgtcac cgcgagttcc tgccgaccct ggctggttta ctgactgatc
     1981 ccgtcattgc gtccgaggcg atgaaaacca tttccgtgat ctgtggcgat gtggcgattc
     2041 aactgctcga ccaacttcct gaagtcgcca aagatgtgat tgtgcgacag cggcttctga
     2101 tggcagttga agagcatctg gcggcttcat gtccggttga ccagaatccc tgggctccgc
     2161 tgcaggccag tgacaaccgg agggtcattg aagtcggtga aaagcgcctt tttgcgctgc
     2221 agccatccct gacgctggat gaagctcaga agaaagcgct ggccaacaag cgtgatgctt
     2281 ttggggtgat ctcaaagttt ctctaccggc cgcgtgaaga tcagattcgg cttacgtatc
     2341 gagaaatgag gtatgaacca ttctggcatg ttgtgtgcca gctctgcctg gattacacga
     2401 gggccaggga agttgatctc aacatcgaac gaccggtaca gagtgttcgc gttgggcgtc
     2461 agattctgac ccccgagcag ggaaggattc gtctgacgat ggatgagcac tgtgtcgagg
     2521 aatgcctgaa ggaagtgttc atcgacgccc agactggcgc tcccggtcca tatcagcgcc
     2581 atctcgccta tccgcggcgc gagatccgtg agaccgagga gttgatggtc gacgaagtca
     2641 tcgtcgttcc ggcgcgcatc aaagcctctc cggtcgtgcg tgatctgctg acggaaattc
     2701 tgaaaccggt gcgggcgacc gagatcaatc acgaacagat cagcctgcat cgggtgaatc
     2761 tcctgtttcg accggtgtat gcgtttgagt ttcagtggga ggacagccag tcgttcgcag
     2821 ttcttgaaat cgatggactc acgggcgaag tggtctcggg aaaagcgctg aagaagaagt
     2881 tcgacgaact cttttccgaa tcatcactgt tcgagctggg ttcagaggcg atcgacctgg
     2941 tcattcccgg tgggggactg gcagccaaac tggctcgtgc ggtgctgggg aaaaactgag
     3001 atcccgtgct tcgaggtgtc agtcggcgcg atagatcacc gagccgtcaa cgaccgtgag
     3061 aacgacgcgt gaacggtgaa tctcttctgg ggcgacggag aacagatcag gttcgatcac
     3121 gatcagatca gccagatagc ccggtcgcaa agcaccgagc tcatgctcgc ggttgaccgc
     3181 gcgggcggcg ccgagggtga agcctgtcag catctcggtg agagtcgccg actggcttgg
     3241 attcagagga tcgccgtggc tttcggggtg aatacgtcgt atgccagtct cgatgccgat
     3301 caacggatcg aagcttgtga cgtccccccc gaacggccag tcactggaaa aagcgacgtc
     3361 cgctcgatga ttgtacaagt ctctcaggcg atacagtttc tctgaacgat cgttgccgat
     3421 ggctggaata gttaccccga gatagttcga gtcgcgcgcg aaccaggaag gttgcaggtt
     3481 ggcgagcacc ccgagatcgg caaaccgagg gatgtccgcg tcgctgatca gatcggcatg
     3541 ggcgatctgg tggcgcagag cggcctgcgg gagcgcggca cgcgcgccgg caatgccgtc
     3601 gagtgcgaga cgaaccgccg cgtccccgac ggcatgcaca tgcaccgccc agtcacgttc
     3661 ggccagtcgc gggatcatct cgagccactc ggcgatgtcc cagaccggtt cgccccgata
     3721 acccgcgcga tccgagtaat cgtgcagcag gtaggcggtt tctccctcga tgacaccatc
     3781 gagaaagagt ttcaacggcc caaaacgaaa gaatggggag tgatgtgcgt ggcggagggt
     3841 ttcggattcc tcgagctgtc ccaggccttt cccgggcgag aaccgcctga ccagggttac
     3901 ccggaaagtc ttcagacgct ccgatcgttc cagttcgagg tagaccgcgg gaaagtgact
     3961 ctttcctgag gcgtcctgca cggaggtgac gccaaaacgt gcggcctgag aggcaaacgc
     4021 atcagctgca ttcagcagtt cggcgtgagt ggcctgtgga atgaggtcgg taaccagttg
     4081 catggcgggc attttccgca gcagtcccgt cggctcgccg gtggtggggt cgcgctcaat
     4141 gatacccccc ggtgggttgg gcgtctgcct ggtgatgccc gccaggcgta gagcggcggt
     4201 gttgcaccag cctgaatggc cgtccatggc tttgaaatag gcaggtcggt cgggaatgac
     4261 cgaatcgagt aactgtcgat gtggcccggg agcgggaaac aggccatgga cccacccgtt
     4321 tcctcgcacg aatgatgcgt ccgggtggcg ctcgagatag gtattgatgt ggcgctggta
     4381 tgaaggccaa tctttcaatc ccatcaacga gcagtccaat agatcggtag cgccgatcag
     4441 aggatgaaga tgcgcgtcgt ggaacccggg gagtacgagt cgactgcgac agtcaaagac
     4501 actggttcga ggtccgcgcc agtccttcat ctggttgtcg gggcccaggg cggcaatgcg
     4561 attgccgcag atcgcaatgg tccactggtg ttctggttgg gaaccgccgc gccagacgtt
     4621 tccgttcacc agcagaagat cggcttccat gagggctcag ctcagcgcgg ggcgagctcg
     4681 gggtgcttcg cgatgaaatt ctgcaggtct tgatgcgact tgaagtttct gactccgtgc
     4741 gctttccagc caaagaagtc gcgcgggtgg tgaaacgtcg tgccgccaaa ggttttctgc
     4801 aggttctcgg cgaacaatct gtccgagttg gaaaagtcgt ctcccggcgt tccgtcaggt
     4861 tccagatcct tgccttcttt ccaggcagag tcgccgacca tgagtgagtt ctgccagtcg
     4921 atcgtcttgt tccaggtggt actggccaga tccgccaatc gacgggccat gccgatatcg
     4981 ggcttgcggt cttcattttc gagttcggca aagtcatagt agtggatggg agctccgatg
     5041 ctggccagac gttcgacggt ataggcaagc gcggcgtcgg cattctgcag agtgacgtgc
     5101 ccgtgagcca ctccggcctg gttggagacg atggcgatga gatatccctc actggccagt
     5161 ttctgcaggg gtcccgtcag caggggcagc aacgcgacat cggttgcgtg gttggccgat
     5221 ggagacccgc tgggggcgat gcgcaacgta ctgtcggcat cgaagaaggc gattcgtaac
     5281 gttgggctgg cgggggggat gaaaagcggt gatatctgcc gtctcttcag agtgggctga
     5341 tctggtgctt cagcgttgat ctgctgaggt tcgaacaagc tgaagaacac ggtggcgaga
     5401 aggacaggca gaagaagttt catggtggcg ttccttgaaa ggcttgggat caggaatggg
     5461 cgtgatcagt ctgcccgaag tcgcggcaaa ctgcaaccag agaccaccgc tcccgggttg
     5521 gtgaacgaca attcgagtga agttcgattt cttcgatgta aaaatgggta atagacaatc
     5581 acaaaattta aatatcgaaa aattgtaagg gtgaaaattc ctgatttgcc gaaaaatatg
     5641 aagtatgacc ttgacgaagt gaacgttgat cagtaagctt cagaaatcga gaccgcactc
     5701 ttaccttccg aaggagcaac ccatgtcccg agttacggaa atgtttggtg aactcacctt
     5761 caatcgcgat gtcatgcgcg agaaactcag ccgcgatacc tacgagaaga tgctcgcgac
     5821 gctgcagacc ggcaactctc tggacgaatc gattgccgat gaggtggctc acgccatgaa
     5881 agaatgggcg atcagcaagg gcgccaccca tttcacccat tggtttcagc ctcagcgcgg
     5941 ggggaccgcc gaaaagcatg atgcctttct cacctatgcg gaaaacggcg agatcattga
     6001 acgtttctcg gccaaacagc tgattcagtc cgaacccgac gcttcctcct ttccttcggg
     6061 tggcattcgc tcgaccttcg aggcgcgtgg ctacaccgcc tgggatccga cttctcccgc
     6121 ctttctcatg gaaaccggac aaaccaagac gctggtcatt ccttccgtgt ttctgtcatg
     6181 gacgggcgat gtcctggatc agaagacacc gttcctgcgc tcgatgcggg cactgaacga
     6241 tgcggggatc aagttgcagc gaatgctggg caaccgaatg gcgaagaaag tgctggtttt
     6301 taccgggccc gagcaggaat acttcgtggt ctcgaagaag ctctatgaca cgcgaccaga
     6361 cctgcagatc tgcggtcgca gtctcttcgg tgctcccccg gcgaaggggc agcagatgga
     6421 ggatcactac ttcggctcga ttcatcgcaa gatgaagaag ttcatggaag acctcgacct
     6481 ggagttgtat cgccggggaa tccccgcgaa gaccagacac aacgaggttt cccccaacca
     6541 gttcgaaatt gcgccgctgt atgaagaagc gaatctggcc atcgaccaca acctgcagat
     6601 gatggacatc atgaaaatgg tcggacagcg ccatgatctc gtcgtcttac tgcacgagaa
     6661 gccctttgcc ggagtcaacg ggtcagggaa gcatgtcaac tggtcgctgg gtgataacac
     6721 cggcgcgaac tatcttgagc cctcggcatc acccctgaag aacatcaatt ttctgttgac
     6781 gctgggcgcg ctgcttctcg gcgtcgacaa gttcggcggc tatctgagag ccacggttgc
     6841 cgatgccggc aacgatcatc gtctcggggc gaacgaagcg cctccggcca tcatgtctgt
     6901 ctatctgggc gagtacctgt cgaagttgct ggccgagatc gaagccatgg gcaagttcaa
     6961 cgagcaatcg atggcgcaca tcaatatggg cgtgcgcaac ctgccacggg tcgtgcgtga
     7021 tacctccgac cgcaatcgca catcgcccat tgcctttacg ggcaacaagt tcgagtttcg
     7081 tgccgtgggt tcatctcaga actgctctga agcagtcaca gcgctgaatc tgctggtgac
     7141 ctacgggtac tatacgattg agaaacgctt gctggcgatg agcggaaaac aggatatcaa
     7201 ggaaaaggca ttggtggtct tgcgtgatgt cttcaaagag accagacggg ttcgcttcga
     7261 aggaaacggt tactcggaag agtggcacaa agaagcggcc aaacgtggac tgccgaatgc
     7321 ccggaatact ccggccgctc tcgatgcg
//
LOCUS       DAORHD010000053         7300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9921_length_7300_cov_3.455072, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000053 DAORHD010000000
VERSION     DAORHD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9921_length_7300_cov_3.455072"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="PKO06_02060"
     CDS             <1..1239
                     /locus_tag="PKO06_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV68452.1"
                     /translation="YFNIVLGDEKVGQKLKEIRLPFGTGLAGWCAKESQPVIVPDASK
                     DPRFFKTADKSSGFVTRSMICVPMRLKDEVIGVIQVLNRTGDIPFNDHDVEILEAVAN
                     QAVSALDNARLYENIQRVYLSTVEVLATAIDAKDPYTHGHSRRVTDYSVAIAEEMGLS
                     KAEIDNVRYAGLLHDVGKIGIKDSIIGKPERLTDEEYNIIKRHPSIGADILKPVEFLS
                     DKIPGVLHHHEYYDGRGYPDHLKGEDIPFIGRVICVADAFDAMTSDRPYRKGLNVNVA
                     IGELKKFSGKQFDPACVDAFLRAFEKKIFQWFEKVPGSDELVLKPDPVGSVRFPEVPA
                     PHGDTGHKESTQPFPPIHPDEKKGTPSSQPFPPIHPEAASKPSSQPFPPIHPEEKKST
                     PSSQPFPPIHPGEPPKDDKK"
     gene            complement(1297..1749)
                     /locus_tag="PKO06_02065"
     CDS             complement(1297..1749)
                     /locus_tag="PKO06_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68453.1"
                     /translation="MLKQLKRAEETLQTRLNEKDEQIRSLSTELDALKSWKEEMGLKL
                     AEFKGASQSNPQMLIFKLLEHNQRLNQALTSKWVNVEKSLTDQLQTSIDQIRKLFAEA
                     EALHRDGLEIISIYESRLPDDTKRKIHEELGRLPGSASKVPVLTERTS"
     gene            complement(1949..3010)
                     /locus_tag="PKO06_02070"
     CDS             complement(1949..3010)
                     /locus_tag="PKO06_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68454.1"
                     /translation="MKRTILLLGLVLVSALVGCDGGGLFNPADAINPYGYIFKNFTTA
                     TAPTLPTDVRSIVYDASKLTLFIGGKGTGLFSVNPNSDPPTAINQATATAELGARIVQ
                     CLAVDKKTGDLFIGTNLGLFRCNKTTGVTTPVAAIAANSSVLSIAQPDNDTLWVGLFD
                     DSADTNSIARSVDSGANFTLFGTDDGMTASAVVQIHADANVTLAAGTGSAGKAGLFKF
                     NKQMLKFAPIDNAPFENGATLINVFGTSWYAGGVNKGLHVSTDSGTTWVELLSSTSSI
                     TPYQILNESDRYWVSSNKGLYLTYNLKDFNLFNTASRLGKDLSRQLASASSMIWMTHT
                     DPTVADGTDGITRGVFAGD"
     gene            3260..3631
                     /locus_tag="PKO06_02075"
     CDS             3260..3631
                     /locus_tag="PKO06_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68455.1"
                     /translation="MDQAEFAAHPMFQNATVRWDESGIPLPLPEVTRRNLFRRAALLV
                     VLIVFTQLYWHGPKTFWSVDAPYSESRYMPPFYQQVQGAHWETFFADVTAALADPPQG
                     VYEERAADRDVHFLEGEGVLQ"
     gene            3635..3940
                     /locus_tag="PKO06_02080"
     CDS             3635..3940
                     /locus_tag="PKO06_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68456.1"
                     /translation="MPRLAEGVASYIFTDEAMAIGPGGMRHRTTSGQETARWEDMVNV
                     KVSSRRNSDDEIELYHLHLRDGRSWSIDITSRWSEDIRTALEIHGVPIIRPAEEKES"
     gene            complement(4158..5069)
                     /locus_tag="PKO06_02085"
     CDS             complement(4158..5069)
                     /locus_tag="PKO06_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68457.1"
                     /translation="MNHRIRSVWLIRSSIVLTVLAVICLSLAVTAPAAAEGYWELVDT
                     KLIDKTQREGTWIKVRRHTDRYVEYEGVLPGSAPKVYRAEWNPPARILIPDGQVLTAV
                     AAIIVSVPPALNHDAGLDVGCCFEHTGIGPGVSSGSGIGGAFAKFGGRAPGKSSDKKI
                     IKAPKKEFGDKQGRMVFNVSVGNGFDNLGATFVYQWKGGPAPKPGVIGGDDQPDDQPE
                     DQPEDKNTDNTAWSVGHPVPWVFHPDGTIDAKGLWNGTWVRSGNDYTVTIEHMGVKDT
                     FTVRFSADGRSFTAYKDGQTYRQGLRR"
     gene            complement(5317..5718)
                     /locus_tag="PKO06_02090"
     CDS             complement(5317..5718)
                     /locus_tag="PKO06_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV68458.1"
                     /translation="MSEAVDMQAEESYYCQRMPDFKIETAIQGSLQIIRTTGYLDDAG
                     GKVLKETCEKLIGEGKAKFVFNLAGTPVINSTGLSMLLDMMVRIIDYNDGQVAICGLS
                     KLTRTALQMTGVLTLAKEFTGEAEAIAGLGA"
     gene            complement(5733..6776)
                     /locus_tag="PKO06_02095"
     CDS             complement(5733..6776)
                     /locus_tag="PKO06_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNV68459.1"
                     /translation="MPLSVFGSRLRVGLLLVLVLVPLMPALSWSGNTHRKIAADALSL
                     MPSEFQERFGSYKKTLMNGAVDPDRLIKDFQNHVYHVHTTRPDVWQVAPDRLQELFQT
                     ITDLLHGKERLPVSGKALKAYLKRSTTELSRDEEIAYQLGLLSHYVADLNQPLHTDGA
                     DIDPVENEYHDLFERDVENQLSQISLLPIRANPVSDLRQRTAEMAKEANSGYRAIGEA
                     YRQGNRAYDLTDLLQKQYSASVQQVIDFWLGVFAFASSPLSTALTRAPDLSVNTISIG
                     HSSSQTRVYRARINLNTASQQELESLPEVGPKKARAIMAARPFRSVYDLRRVKGFGQN
                     LVDKLIDQVTIDP"
     gene            6939..>7300
                     /locus_tag="PKO06_02100"
     CDS             6939..>7300
                     /locus_tag="PKO06_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68460.1"
                     /translation="MSTTTPTSSDKLPEIQTEAGAAPVLPPLPTVDPSVQPTVGEDLE
                     RLRQIDCLKATIVRWKTGTHPDTVIEQVLARRLSLEESRFLTPFPLRVLTALALVFAG
                     CAGAWGGLWFVCWQLGFT"
BASE COUNT         1649 a         1914 c         2018 g         1719 t
ORIGIN      
        1 tatttcaaca tcgtactggg tgatgaaaag gtcgggcaga aactgaaaga gattcgactc
       61 cccttcggca ccggtctggc cggctggtgt gcgaaagaga gccagcccgt tatcgtgccc
      121 gacgcgtcga aagaccctcg atttttcaaa actgccgata aaagctcggg gttcgtgacc
      181 cgctccatga tctgcgtgcc gatgcgcctg aaagacgagg tcatcggggt cattcaggtt
      241 ctgaaccgta ccggcgatat tccgtttaat gaccacgatg tcgagatcct tgaagcggtt
      301 gccaatcagg cggtttcggc tctcgacaat gcgcggttgt atgaaaacat tcagagagtg
      361 tacctgtcga cggtcgaagt tctggccacg gccatcgatg ccaaggaccc ctatactcat
      421 ggtcattccc gccgcgtgac cgattattcg gtggcgattg ccgaagagat ggggctcagt
      481 aaagccgaga tcgacaatgt ccggtacgcc gggctgctgc acgacgtcgg caagatcggc
      541 atcaaggaca gcattatcgg aaaacctgaa cggttgaccg atgaagagta caacatcatc
      601 aaacgtcatc ccagcattgg tgctgatatc ttgaagcccg tcgaattcct gagcgacaag
      661 attcccggcg ttttgcatca tcacgagtat tacgatgggc gtgggtatcc cgatcatctc
      721 aagggcgaag atatcccatt catcggaaga gtgatctgtg tggccgatgc gttcgatgcc
      781 atgacctcgg accgcccata ccgcaagggg ttaaacgtga acgtcgccat tggtgaactg
      841 aaaaagtttt cgggaaaaca gttcgacccc gcctgcgttg atgcgttcct gcgtgcattt
      901 gaaaagaaga tctttcaatg gtttgaaaag gttccaggct cagacgaact ggtactgaaa
      961 ccagatccgg tcggcagtgt gcgcttcccc gaagtgccgg caccgcacgg tgataccggg
     1021 cacaaagaaa gtacgcagcc gttcccgccc atccacccgg atgaaaagaa aggcactcct
     1081 tcgagtcaac cattcccgcc gattcaccct gaagcggcct caaaaccctc ttcgcaaccc
     1141 tttcctccga ttcatcccga ggaaaagaag agcacaccct cgagtcagcc gttcccgccg
     1201 attcatccag gcgaaccacc gaaagacgac aaaaaataaa ccccgagcca gaagcccggg
     1261 gttctttctt ttatctgatc aaagacaaag ctctgatcaa ctcgtacgtt cagtcaaaac
     1321 cggcaccttg ctcgcagagc ccggcagacg tccaagctct tcatgaatct tccgtttcgt
     1381 gtcatcggga agtcgcgatt cgtagatcga gatgatctcg agcccgtcac gatgcagggc
     1441 ttcggcttcg gcaaacagct ttcgaatctg gtcgatgctg gtctgcagct gatcggtcag
     1501 gctcttctcg acgtttaccc actttgaagt cagagcctgg ttcaaacgct ggttgtgttc
     1561 gagcagcttg aagatcagca tctgcgggtt gctctgtgaa gctcccttga actcggccag
     1621 cttcagcccc atctcttctt tccagctttt caaggcgtcg agttcggtac tcagactgcg
     1681 aatctgctca tccttttcat tcagtctggt ctgtaatgtt tcttcggcgc gcttcagctg
     1741 cttcaacata tcttcggtaa accccgcctt gaaagactca tgctgtgagc gggtttcctg
     1801 cagtttctgc tcgtacgaat ccttgatcag ctgcagctcg cggtttttga attagaacga
     1861 gatcttcgtg ccgcccacaa acagcagcgt gatgacgaga atgatgaaca gctgagtaat
     1921 gagatccatg ctggtgtcgc gccccgcctc agtcgcccgc aaacacgccg cgggtaatgc
     1981 catctgtacc gtcagcgacc gttggatcag tgtgggtcat ccagatcatg gaggaggcag
     2041 aggcaagctg tcgagacagg tccttgccaa gacgtgacgc cgtgttgaag aggttgaagt
     2101 ccttgagatt ataggttaag tacaagccct tgtttgaaga aacccagtat ctgtcacttt
     2161 cgttaaggat ttgataagga gtaattgatg atgtggagga gaggagttct acccacgtag
     2221 ttccgctgtc ggtcgaaaca tgcaggccct tgttcactcc gccggcatac caggaagtac
     2281 caaatacatt gatgagtgtg gcgccatttt caaagggtgc gttatcgata ggagcgaatt
     2341 tcagcatctg cttgttgaac ttgaacaacc ctgctttgcc ggcagagcca gttccagctg
     2401 caagcgtgac gtttgcatca gcgtgaatct gcacaaccgc gctggccgtc attccgtcat
     2461 cagttccaaa caaggtgaag tttgccccgg aatcaacaga gcgagcaata gagttggtat
     2521 ctgcggagtc atcgaacagc ccaacccaga gtgtgtcgtt atcgggctgt gctattgaaa
     2581 gcacagagct gttggcagca attgcagcga ctggcgttgt gacaccggtt gtcttgttgc
     2641 accgaaacaa tccaagattc gtgccgatga acaaatcacc ggtcttcttg tcaaccgcga
     2701 gacattgaac gattcgagct ccaagctcgg ctgttgctgt tgcctgattg atggcggttg
     2761 gtggatctga gtttggattc acggaaaaca gaccggttcc tttgccaccg atgaacagcg
     2821 tgagcttgct ggcatcgtaa acgatggatc gaacatctgt cggtaaagtg ggggcagtgg
     2881 cagtcgtaaa attcttgaag atgtagccgt aggggttgat ggcgtcggcg gggttgaaga
     2941 gaccgccacc atcgcagccg accagggcag agaccagaac aagaccgaga agaagaatcg
     3001 tgcgtttcac ggttaaacct ccgtgataat ggagttggca agggctttga gtgtagctca
     3061 gcatcagcgc gttggcaact ttccatcgaa gcagaggcaa tgaagctgtt acgatccata
     3121 gcctgttcca cgcggataga gtctgcatct ggcctgcgtc gcaaactgac tttcgtgttt
     3181 gagtccttga gattgtggta gccttttgtt cgatagccga tgatgcgcga gagtacttcg
     3241 tgaacaggga ggagctgtca tggatcaagc agaatttgcg gcacatccga tgtttcagaa
     3301 tgccaccgtt cgctgggatg aatcgggaat tccacttcct ctccctgaag ttactcgacg
     3361 caacctgttt cgaagagcgg ctttattggt tgtcctcatt gttttcacgc agctgtactg
     3421 gcatggcccc aagacttttt ggagtgtcga tgcgccctat tctgagtcgc gttatatgcc
     3481 tccgttctat cagcaggttc agggggcgca ttgggaaacg ttctttgccg atgtcaccgc
     3541 ggctcttgct gatcctccac agggtgtcta tgaggaacga gcggcagatc gagatgtaca
     3601 ttttctcgaa ggggaaggtg ttttgcagta gttgatgcct cgcctggccg aaggagtcgc
     3661 cagctacatt ttcaccgatg aagccatggc tatcggtccc ggaggcatgc gtcatcgaac
     3721 gacgagcggt caggaaaccg cgcgctggga agacatggtg aacgtgaagg tatcgagtcg
     3781 tcgcaactcg gacgatgaaa tcgagttgta tcaccttcac cttcgcgatg ggcggtcatg
     3841 gtcgatcgat atcacttctc gatggtcaga agatattcgc accgctctcg agattcatgg
     3901 tgttcccatt attcgaccag ccgaagaaaa ggaatcctga gtttcgtgcg tatgctaaat
     3961 gtacgagttg atagaataga aaaaccacat tcagtaaaca tgtgaagcgt tgagttctga
     4021 ggatgtgaaa gagcgatcag cgaagacaga aacgagtctg cggaaaaaac cgcgatcgaa
     4081 ccgatgtggt tcagatcgcg gtttgttctg cggggtggtt cttgaactta tgattcagag
     4141 tttccccgtg gtctgagtta acggcgaaga ccttgtcgat aggtctgccc atccttgtag
     4201 gcggtgaatg agcggccgtc ggcgctgaat cgaacggtaa aggtatcctt gacccccatg
     4261 tgttcaatgg tcacggtata gtcgttgccg ctccgaaccc aggttccgtt ccacaagccc
     4321 ttggcatcaa tggttccgtc cgggtgaaac acccatggca cggggtgtcc gacgctccag
     4381 gcggtattgt ccgtgttttt gtcttcgggc tgatcttctg gttgatcgtc aggctgatca
     4441 tcgccaccga tcacgccagg tttgggcgca gggccaccct tccactgata gacaaaggtg
     4501 gctccgagat tgtcaaatcc gtttccgacg ctcacattga acaccatgcg accctgcttg
     4561 tctccgaact ctttcttggg agctttgatg atcttcttgt ctgaactctt gccgggtgct
     4621 ctgccaccga acttcgcaaa agcacctccg attcctgatc cgctgctcac gcctggccca
     4681 atgccggtgt gttcgaagca gcaacccaca tccagccctg catcgtgatt cagtgcggga
     4741 ggaacggaaa cgatgatggc agcaactgct gtgagtactt ggccatctgg aatcagaatc
     4801 ctggctggcg gattccattc ggctcgatac acctttggag cagagccggg aagtactcct
     4861 tcatattcga catagcggtc tgtgtgacgc cgaaccttga tccaggttcc ctctcgttgc
     4921 gttttgtcga tcagcttcgt atccaccaat tcccagtatc cctctgcggc tgccggggca
     4981 gtgactgcga gactcagaca gatgacggcg agaacggtca gaacaataga agaacgaatc
     5041 aaccagactg accggatacg atgattcatg acgtaggtcc tccagctctc gatgaaatac
     5101 ttctgtgcgg atcatcggta agagtagcaa aaacccgctg ttttgcctag ttgccgccta
     5161 gtgtcggtcg tggcgcgcaa acgctgaagt cacgaggtgt tgcgccgctt cgtttctcat
     5221 cagatgagag aaccgcgccg tacgggagag caaagagtct tcagaagcag agggtaagcg
     5281 cgacagaaag atccgtgcga aaatttgggg gcgaaatcag gcgccgaggc cggcgatggc
     5341 ttcggcttca ccggtgaact ctttggccag agtcaacacg ccggtcatct gcagggcggt
     5401 gcgggtgagc ttggaaagac cgcagatcgc gacctggccg tcgttgtaat cgatgatgcg
     5461 aaccatcatg tcgagcaaca tcgacagacc ggtactgttg atgaccgggg ttccggcgag
     5521 gttgaagacg aacttggctt tgccttcacc gatcagtttc tcgcaggttt ctttgagaac
     5581 cttgccgccg gcgtcatcaa gatacccggt cgtgcgaatg atctgcaggc tgccctgaat
     5641 tgcagtttcg attttaaagt ctggcatacg ctggcaataa taggattctt cggcctgcat
     5701 gtcaactgct tctgacatgc cgctcacagg cttcacggat cgatggtgac ttgatcgatg
     5761 agcttgtcga ccagattctg gccgaacccc ttcacccgtc tcagatcata gacgctgcga
     5821 aacggacgtg ccgccatgat ggcacgggct tttttcggac ccacttcggg caatgattcc
     5881 agttcctgtt gcgatgcggt attcaggtta atgcgcgctc gatacacacg tgtctgtgac
     5941 gacgaatgtc ctatcgatat tgtattaaca gaaagatcag gagctcgtgt aagagctgtt
     6001 gaaagtggtg aactggcaaa ggcaaaaacc ccgagccaga agtcgatgac ctgctgcacc
     6061 gaagccgaat actgcttttg cagcagatca gtcaaatcgt aggcgcgatt tccctgtcga
     6121 tacgcttctc cgattgcccg gtatccgcta ttcgcctcct tcgccatttc cgccgttcgt
     6181 tgtctcaggt cagaaacggg gttcgctctg atgggaagaa gggagatctg cgacagctga
     6241 ttttcgacgt cgcgttcgaa caggtcgtga tactcatttt ccacggggtc gatatcagcg
     6301 ccatcggtat gcagcggctg attcagatcg gcgacgtagt gactcagaag cccgagttga
     6361 taggcgattt cttcgtcgcg ggaaagctcg gttgtcgatc gcttgagata ggctttgagt
     6421 gccttgcccg agacggggag ccgctccttg ccatgcagca ggtcggtgat cgtctgaaac
     6481 agctcctgca agcgatctgg ggcgacctgc cagacatcgg gtcgcgtggt atgcacgtga
     6541 tagacgtgat tctgaaagtc tttgatcagg cggtcagggt cgacggcacc attcatcaac
     6601 gtttttttgt acgaaccgaa acgctcctga aactcggatg gcatcagtga cagcgcgtct
     6661 gcggcaatct tgcgatgcgt gtttcccgac cagctcaggg caggcatcag gggaaccagc
     6721 accagtacga gcagcaaccc cactcgaagg cgagaaccaa acacggagag cggcatgagc
     6781 gtatcctttg gcctactgac attgagtata cgccgaaagc aatcggcttc tcaatgcgac
     6841 gattcgtcgt tctccgggtg aacaaatctc tcgccgaaat gccagctgac gttctgcggc
     6901 ggaaaaggta tattgccttc agcgagtaac gaacgctcat gagcacaacc actccgactt
     6961 catctgacaa gctgcctgaa atccaaaccg aagcaggtgc ggctccggtt cttccacctc
     7021 tcccgacggt cgatccctcg gtacagccca ccgttggtga agatctcgag cggttgcgac
     7081 aaatcgactg cctgaaggcc acgatcgtgc gctggaaaac gggaactcac cccgataccg
     7141 tcatcgagca ggtgctcgca cgccgcctgt cacttgagga aagccgattt ctgacgccgt
     7201 ttcctctgcg ggtgctgacg gcgctggccc tggtgttcgc cggctgcgca ggtgcctggg
     7261 gtggactctg gtttgtctgc tggcagctgg gtttcacgga
//
LOCUS       DAORHD010000054         7280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_9967_length_7280_cov_3.311696, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000054 DAORHD010000000
VERSION     DAORHD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9967_length_7280_cov_3.311696"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKO06_02105"
     CDS             <1..576
                     /locus_tag="PKO06_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68461.1"
                     /translation="PNSPIKIQKTASGIELHWDPFMGCGPARFIPAAFLMFWLCGWLV
                     GELFAISAFIMCILKMQIIPAIFLAIWLTGWSVGGYFAIRVLRGMLQPSRPERLVFEE
                     HLLQHDAGLNPMTWMSQTSKLAKRRTTTGEMVEIRRTDVQNIRMEKGSKGRLRLIVDC
                     EAGSVEMGMMLSHADKQWVFQRLRDWWGRET"
     gene            complement(588..1241)
                     /locus_tag="PKO06_02110"
     CDS             complement(588..1241)
                     /locus_tag="PKO06_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68462.1"
                     /translation="MLYSFGTALSERVDDLLCYTFFMRQNLYQEPETHLDCDEIDRFK
                     AALAAIPDRPRTAGFDAQRREDAISSLTLARTVAFLMYSMLAPLITLIVLMMPPFPSW
                     PRQTLFLIALGLFTSLEYLLFIAVQRGWLPSPDPEHVSPFSEHQIEFAALCLSVTPWL
                     LRFDPSRFLVFQGVINGSLIIAGIVFFITKSMLSRRWRWGVLRVLILPIVQLVWLFH"
     gene            1348..4950
                     /locus_tag="PKO06_02115"
     CDS             1348..4950
                     /locus_tag="PKO06_02115"
                     /inference="COORDINATES: protein motif:HMM:NF040115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68463.1"
                     /translation="MASIITGMSTDQIAALTTTQIRNLTTADFKAMSTAQVKALTSDQ
                     VPAIETQDLFVLSTAQIQALDTYDIQVMSSDQVKIMTSAQIKALTSDQMNALEFEDFA
                     AMSSAQIKAIDAEDLNVVISAVVTAFSTDQFAALTSTQFAGLNTTSLSVLMPTEIAQI
                     STAQVKGLTTAQIAALDSDQIQAFQPAVLGAMSAAQIAAIKTEQISLLSTAQIGGLSP
                     TQIKALTTAQAKELSATQIGVLSGAQLKAIEIIDLQQFGTGQIDALTTSQISELTSTQ
                     LGALNSTQLGAFGTEDIGAISTAQIKGMTTAQIASLSSTQTLAFKPEVLGAMTVAQVP
                     AIPASYVGSLSTAQFAALSTAHIKALTTSQVAQLTTAQIGTLDAAHIKAFENIDLQQF
                     GSDQINALSTAQISALTSAQLGGFTTGQLVNFGTEDIDAISTAQIKGMTTAQIASLSS
                     TQTLAFKPEVLGAMTVAQVPAIPASYVGSLSTAQFAALSTAHIKALTTSQVAQLTTAQ
                     IGTLDAAHIKAFENIDLQQFGSSQINALTTAQISALTSSQLGGLTTTQLVDFGTEDMD
                     AITTAQIKGMTTAQIASLSSTQLQAFKPVTIGAMTPAQVAAINSAHISTLLTDQFAAL
                     TSAQVKAMTSSQVGQISTAQIGVLSDANLKAIDVVDLQTWGSDQINALSTTQMSALTS
                     SQLGGLTTTQLVDFGTEDIDAITTAQIKGMTTAQIASLDSDQIPAFKAATLGAMTSAQ
                     IAAISTATIPLISTTQMAGLTSAQIAALTTAQVQQLTTAQIASLSVTQVKGMETGDIA
                     VLSTLQVQTLGSDQIGAMLPTQMKGFSTDHLAVLSTDEIAGMTSAQIAALSTLQVKSL
                     GSDQISAIEIADITAMTAAQIASMTTEQVYNGLRTAQVAALTATQVKAMTTDQVKNLS
                     TDQIDALKTNQLSAMTSGQLQALTTDQLDGLRTDQFGALSSTQLAALKTDQVRDGLTP
                     AQLATITSTQIKGFTASQVNAMTTDQVAAFTPTVFPFLSSAQVAALTTAHIQNSLSTD
                     HIIALTTAQVKGLTSSQIDAMSTAQLSNLQTEDFVALSSSQIAAISTTQIQTGGLTNT
                     QIEKLTTAQVKGLTSAQIQAFSTAQVALIEPEDLGAFTTAQIQWLTTQQVQQALGSSQ
                     IQALKITQIPALKTDQIQALTSTQVGYLTSTQVRALTPEQISVMSTDQISQLAI"
     gene            5068..6882
                     /locus_tag="PKO06_02120"
     CDS             5068..6882
                     /locus_tag="PKO06_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013792995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68464.1"
                     /translation="MVTIDLLTAYERAWKQQLSFQDLLQVAEALGASGAGSQAAILYH
                     IWLRRTQGPQLHIAWFNFGALLFSEGDLAGARDAYQQALKLAPGFVQPRFNLGLTYER
                     LGQPDLAVNEWRVIDDSVSPSQEDQKPLLIMALNNLGRLLEEKKHYYAAHDYLVKSLM
                     LNPEQSDVLHHLVYLREKMCAWPVYFTIPGVRPELMKQSTSALAMIGLSDDPQEQLDA
                     AKQYVKTKVRSDVPALRPEKTYGHKKIRIGYCSSDFCMHPVSMLTVQLFELHNRDEFE
                     VYGFCWTRQGHSALRQRVMDAMDHFIRIDEMSDEAAAKKIRECEIDILVDLHGQTLGA
                     RANMLAYRPAPIQITYLGLPATTGLPSIDYVIADRFLIPAEHVRFYSEKPLYMPDVYQ
                     VSDRKRQSTEPPTREQCGLPADAFVFCSFNNNYKYTPEMFEAWMNILRRTPESVLWLL
                     ADNPYAQENLKREARLKGVEASRLIFATRVLPEQYLARYAVADLFLDSFPFNAGTTAN
                     DALWMGLPVLTRTGRTFASRMAGALLTAAGLPELITYDVKAYEEKAVELAANRSHCRK
                     LREHLQSVREQGVLFDTPRFVRHLEQRLQSLMESGCEK"
     gene            complement(7095..>7280)
                     /locus_tag="PKO06_02125"
     CDS             complement(7095..>7280)
                     /locus_tag="PKO06_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68465.1"
                     /translation="VNDKIPANRNFRLNIIDKIEDLLIEQESVTNIPYDIVFFNRKDF
                     ERNKKIRTAGSAALENL"
BASE COUNT         1729 a         1880 c         2063 g         1608 t
ORIGIN      
        1 ccgaattcgc cgatcaaaat tcaaaaaacc gcgagcggta tcgagctgca ctgggaccct
       61 ttcatgggat gcggtccggc gcgtttcatt ccggctgcct ttctgatgtt ctggctctgt
      121 ggctggctgg tgggggagct ttttgccatt tccgccttca tcatgtgcat tctgaagatg
      181 cagatcattc cggccatctt tctggcgatc tggctgacag gatggagtgt gggcggctac
      241 tttgccattc gtgtgctgcg tgggatgctg cagccctcac gtcccgagcg actggtcttc
      301 gaggagcacc tgctgcaaca cgatgcggga ctgaacccga tgacctggat gtcgcagacc
      361 tcgaagctgg cgaaacgacg cacgacgact ggagagatgg tcgagattcg ccggacagat
      421 gtgcagaaca ttcggatgga aaaaggatcg aaagggcgtt tgcgactgat tgtcgattgt
      481 gaagcaggca gcgtggaaat gggaatgatg ctgagtcacg cggataaaca atgggtgttt
      541 cagcggttgc gcgactggtg gggcagggaa acgtgatggc gacgctacta atgaaacagc
      601 cagacgagct gcacgatcgg caggatcaga acccgcagga cgccccagcg ccagcgccga
      661 ctgagcatgg atttggtgat gaagaatacg attccggcaa tgatcagcga accgttgatt
      721 acgccctgaa acacgaggaa tctggaggga tcgaaacgca acagccaggg cgtgaccgac
      781 agacagagtg cggcaaactc gatttgatgc tcgctgaagg ggctgacgtg ttcgggatcg
      841 ggcgagggga gccatcctcg ctgaaccgcg atgaatagaa gatattcgag ggaggtgaaa
      901 agaccgagag cgatcaggaa cagcgtctga cgcggccagc tgggaaacgg cggcatcatc
      961 aggacaatca gggtgatgag cggtgccagc atggaataca tcagaaatgc gaccgtgcgg
     1021 gccagtgtca gtgaagagat cgcatcttcg cggcgttgcg cgtcgaaccc ggcggttcgc
     1081 ggccgatcgg ggatcgcggc aagagccgcc ttgaaccgat cgatctcatc gcaatcgaga
     1141 tgagtttcgg gttcctgata cagattctgg cgcatgaaga aagtatagca caacaggtca
     1201 tcgacacgct ctgatagcgc agttccgaat gagtagagca acgtcgttct cagttttttt
     1261 tcggaactgc cgataggagt actggaaaac tgtctttccg tgagtcctgg ggaacacatt
     1321 tctaaggatt agaaaaaggg gatagctatg gcttcgatta ttacagggat gtccacagat
     1381 caaattgcgg cgctgacgac cacgcagatt cggaacctga cgacggctga tttcaaagcc
     1441 atgtctacgg ctcaggtcaa agcattgacc agcgatcagg tgccggcaat tgaaacccag
     1501 gatctcttcg tcttgagcac cgcgcagatt caggctctag acacgtatga catccaggtc
     1561 atgtcatcag atcaggtcaa gatcatgact tcggcccaga tcaaagcgct gacttccgac
     1621 caaatgaacg cgttggagtt tgaggacttt gccgccatgt cgtcggcaca gatcaaggca
     1681 attgatgcgg aagatctcaa tgtggttatc tctgccgtgg tcactgcatt ttcgaccgat
     1741 cagtttgcgg cactgacctc cactcagttt gcgggtctga atacgacatc actctctgtc
     1801 ctgatgccga ccgaaattgc ccagatcagt acggcccagg tcaaggggct gacaacggcg
     1861 cagattgccg cactcgacag cgatcagatt caggcattcc aacccgccgt tcttggggcg
     1921 atgtcggcag ctcagattgc agccatcaaa acggaacaga tctcgctgct ctctacggcg
     1981 caaatcgggg gactgtctcc aacccagatc aaagcgttga cgacggctca ggccaaagag
     2041 ctttctgcca cacagattgg cgtactctct ggtgcgcaac tgaaagcgat cgaaatcatt
     2101 gatttacagc agtttggaac tggtcagatc gatgccttga cgacgtctca gatcagcgag
     2161 ctaacctcga ctcaactggg tgcattgaat tccacgcaac ttggcgcttt tggcaccgaa
     2221 gatatcggcg cgatatccac agcgcagatc aagggcatga cgaccgcgca gattgcatcg
     2281 ctgagctcga cgcagacact ggctttcaaa cctgaagttc tgggagcgat gacggtggct
     2341 caggtgccgg cgattccggc gtcctatgtc ggatctctat cgaccgctca gttcgcggct
     2401 ttgtcgacag cgcatatcaa ggctttgacg acgtctcagg tagcgcaact gactaccgcg
     2461 cagatcggaa cacttgatgc tgcccacatc aaagcgttcg aaaacattga cctgcaacag
     2521 tttggatcag atcagatcaa tgccttgtca acggctcaga tctcggcact tacctcggca
     2581 cagctaggtg ggttcacgac cggccagctt gtcaacttcg gcactgaaga tatcgacgcg
     2641 atatctaccg cgcagatcaa gggcatgacg accgcgcaga ttgcttcgct gagctcgacg
     2701 cagacactgg ctttcaaacc tgaggttctg ggagcgatga cggtggctca ggtgccggcg
     2761 attccggcgt cctatgtcgg atctctatcg accgctcagt tcgcggcttt gtcgacagcg
     2821 catatcaagg ctttgacgac gtctcaggta gcgcaactga ctaccgcgca gatcggaaca
     2881 cttgatgctg cccacatcaa agcgttcgaa aacattgacc tgcaacagtt tggttcaagt
     2941 cagatcaatg ccctgacaac ggctcagatt tcggcactca cctcgagcca gctgggcggg
     3001 ttgacaacga cacagcttgt cgacttcggc actgaggata tggatgcgat tacaaccgcg
     3061 cagatcaagg gcatgacgac cgcgcagatc gcatcgctgt ctagtacaca gctgcaagcg
     3121 ttcaaaccgg tcacgattgg agcgatgacc ccagcccagg ttgcggcgat caacagtgca
     3181 catatctcaa ccttgttgac cgatcagttt gccgccttga caagtgctca ggttaaagcg
     3241 atgacgtctt cgcaggtcgg acaaatttcg acggctcaga ttggggtgct gtctgatgcg
     3301 aacctgaagg ccatcgacgt cgttgacctg caaacgtggg gctcagacca gattaacgct
     3361 ctttccacca cccagatgtc cgcgctcacc tcgagccagc tgggcgggtt gacaacgacg
     3421 cagcttgtcg acttcggtac agaagatatc gatgccatca cgaccgcgca gatcaagggc
     3481 atgacgactg cgcagattgc ctctctcgat agtgatcaaa ttcctgcttt caaggctgct
     3541 actttgggcg cgatgacgtc tgctcaaatt gcggccatta gtactgcgac gatcccgttg
     3601 atctcgacga cgcaaatggc agggctcacc agtgctcaga ttgccgcact gacgacagcg
     3661 caagtgcagc aactgacgac ggcgcagatc gcctcactgt cggtcaccca ggtcaaaggt
     3721 atggaaacgg gtgatattgc ggtgctgagt acgctgcagg tgcagacatt gggctcggat
     3781 cagatcggag ccatgctacc cacgcagatg aaggggttca gtacggacca ccttgccgtg
     3841 ctatcgacgg atgagattgc tgggatgacg agtgctcaga ttgccgctct gtcgacactt
     3901 caggtcaaga gtctgggcag cgaccagatc agcgccatcg agatcgcgga catcacggcc
     3961 atgacggcgg cgcagatcgc ctcgatgacg acagagcagg tctataatgg cttgcgaacg
     4021 gcacaggtag ccgcgttgac ggcgactcag gtgaaagcca tgacgacgga tcaggtgaaa
     4081 aacctctcga ccgatcaaat cgatgctctg aagacgaatc agttgtcggc aatgaccagt
     4141 ggccagttgc aggcgctgac aaccgatcaa cttgatggac tgcgaacgga tcagttcggt
     4201 gcactgtcga gcactcagtt ggcggcactg aagacagatc aggtgaggga tggtctcacg
     4261 cctgcgcagt tagcgaccat cacgagcact cagatcaaag ggttcaccgc atcgcaagtg
     4321 aatgccatga cgacagatca ggttgccgcg tttacgccga cggtcttccc gttcttgtcc
     4381 tcagctcagg ttgcggctct cacgactgct cacattcaga actctttgag cactgaccac
     4441 atcattgcct tgacgacggc acaggtcaag gggttgacga gtagtcagat cgatgccatg
     4501 tccacggctc aactctcgaa cctgcagaca gaagactttg tggctctgtc atcttcacag
     4561 attgctgcga tcagtacgac gcagatccag accggggggt tgacgaatac ccagatcgag
     4621 aaactgacga ccgcgcaggt gaagggcctg acttcggctc agatacaggc attttcaacc
     4681 gcgcaagtcg ccctgattga acccgaggat ctgggagcgt ttaccaccgc gcagatacaa
     4741 tggctgacca cacaacaggt gcaacaggca ctcggatcga gccagattca ggcattgaag
     4801 attactcaga ttcccgcatt gaagacggat cagatacagg cactgacatc gacgcaggtc
     4861 ggctatctga catcgaccca ggtgagggct ctcacaccgg agcagatatc ggttatgtcc
     4921 acggaccaga tctcgcagtt ggcgatctga tctttttcag cgttcatcgg acccccgcgt
     4981 catgtgacgc gggggttttc attcggggga aaaacgtgta caacatttca cggaagattc
     5041 agcgacacag gggacatgga gacgatcatg gtgaccatcg atctgctgac tgcgtatgaa
     5101 cgtgcatgga agcaacaact ctcatttcag gatcttcttc aagtagcaga agctctggga
     5161 gcgtcagggg caggcagtca ggcggcaatt ctctaccaca tctggttgag acggacgcag
     5221 gggccgcagc tgcacatcgc ctggtttaac ttcggagcgt tgcttttctc tgaaggcgat
     5281 cttgcggggg cgcgggatgc ctatcaacag gcgttgaagc tcgcgcctgg atttgtccag
     5341 ccaaggttca atcttggttt gacatatgaa cggcttggac agcctgatct ggcggtaaac
     5401 gagtggagag tcatcgatga ttccgtcagt cctagtcagg aagaccagaa accacttctg
     5461 ataatggctc tcaataatct tggtcgcctt cttgaagaga aaaagcacta ttacgcggcg
     5521 catgactatc tcgtgaaaag tctgatgttg aatccggaac agagcgacgt gcttcaccat
     5581 ctggtgtatc tcagagaaaa aatgtgcgcc tggcctgtct atttcactat tccgggggtg
     5641 cgccctgaat tgatgaagca gtcaacatcg gcactggcca tgattgggct ttccgatgac
     5701 ccgcaggaac agctggatgc ggcgaaacag tatgtcaaga cgaaagtccg atctgatgtt
     5761 cccgcacttc ggcctgagaa aacgtatggg cacaagaaga ttcgcatcgg ctattgttcg
     5821 tctgatttct gcatgcatcc ggtgtcgatg ttgaccgtac aactgtttga actgcacaat
     5881 cgcgatgaat tcgaggttta cggattctgc tggacacgcc aggggcactc ggcgttgcga
     5941 cagcgagtca tggatgccat ggatcatttc attcgtatcg atgagatgag cgatgaagcg
     6001 gcggcgaaaa agattcggga gtgcgaaatc gacattctcg ttgatttgca cggacaaacg
     6061 ctgggggctc gtgccaatat gctggcgtac cggccagctc cgattcagat cacctatctg
     6121 ggcctgccgg cgacgacagg attgccatcc atagactatg tcatcgctga ccgatttctg
     6181 ataccggctg aacatgtacg gttctattca gaaaaaccgc tgtacatgcc tgacgtctat
     6241 caggtcagcg atcgaaagcg gcaaagcaca gaaccgccga cgcgagaaca gtgcggactg
     6301 cctgctgatg cttttgtctt ttgctcgttc aacaacaact acaagtatac gccagagatg
     6361 ttcgaggcgt ggatgaacat tctgcggcgc actcctgaaa gtgtgctgtg gctgttggct
     6421 gataacccgt atgctcagga aaatctcaag cgcgaagcgc gcttgaaagg ggtcgaggct
     6481 tcacgcctga tctttgcgac gcgtgtcctg cccgaacagt atctggcccg ctatgccgtt
     6541 gccgacctgt ttctcgattc ttttcccttc aacgccggaa cgaccgcgaa tgatgcgctc
     6601 tggatgggct tgccggtgtt gacacgcacc ggtcgaacat tcgcgtcacg catggctgga
     6661 gcccttctga cggcagccgg acttccggag ttgattacgt atgatgtgaa agcatacgaa
     6721 gagaaggcgg ttgaattggc ggcgaatcgt tcccattgtc gaaaattgcg ggaacatctt
     6781 cagtcggtgc gcgaacaggg ggtgcttttt gatacgccca ggttcgtgcg tcatctggaa
     6841 cagcgtttgc aaagtcttat ggaaagcggt tgtgagaagt agcggcgttg gttccccgga
     6901 tcgctttctc cgcgtgccca cgtgagtgat cacgcgcgaa tcgggcctgc tcgcggattg
     6961 tgatggaaaa atgtttgact ggaagagaga tgtgtgttag agtgttgttt caaagcaggt
     7021 atttacctcg cctcgagctt cggcgaagag gttgattgtc cggctctcgg gctgggcaag
     7081 cctgaataaa actgttacaa gttttcaagg gcggcagaac ctgccgtcct tattttttta
     7141 tttcgttcaa aatccttgcg gttgaagaaa acaatgtcat atggtatatt ggtgacagat
     7201 tcctgctcta tcaacaagtc ttcaatttta tcaataatat tcaaccgaaa gtttctgtta
     7261 gcaggtattt tatcattaac
//
LOCUS       DAORHD010000055         7199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10134_length_7199_cov_3.619541, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000055 DAORHD010000000
VERSION     DAORHD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10134_length_7199_cov_3.619541"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKO06_02130"
     CDS             complement(<1..745)
                     /locus_tag="PKO06_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68466.1"
                     /translation="MRCACGFENEGGNKFCNMCGMALPAEGDAGSPMERAPLELDLSL
                     DLGDGSPSSGGETSGLDLNLDLGTPAADGGLDLGGINLDGGSKPAGLDLDLGSSEAGI
                     ASAGGLNLESQPITEGDLSFDLHAQPAALDTGSIEAPAADGLDLNLDTTDSGALDLNL
                     DTQEPVGLDLGASGGELNLDLGEPDLPPMKPQPAKPAARPEPPTVPPVEPKVASVVRP
                     EPKPMAPPPKAEVKPVAPPPPPPPPPPPPP"
     gene            complement(975..2693)
                     /gene="ptsP"
                     /locus_tag="PKO06_02135"
     CDS             complement(975..2693)
                     /gene="ptsP"
                     /locus_tag="PKO06_02135"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV68467.1"
                     /translation="MTRPTPLRLVGITGSVGTCVARAYVFRRRLEIERRPIEETAVAR
                     ELERLAEAIELTRRDILAVQQEALSKHGQKYAAIFDSHLLMLSDPSFRPEVEKQVQKR
                     RLNIEVIVQDVVNSFLQRFLAIEDEYLRERAVDMQDVGDRILRHLLGLEGPAAEIGAE
                     PFVLLASDVTPTELLEFAKGHLRGICLDSGGATSHVAILAGALGIPSVFGLTDLSRVA
                     HTGDQILVDTRSQGLVVLHPAAEQVATIAPVAEAPQMPAEGLVTADGYRFTVGANVAR
                     VEELAMLERQNIRRIGLFRSEFLFMESVDLPSETFQEQIYRQVVETARDMAVLRTIDI
                     GSDKPVKYLPFPREVNPAMGFRSIRFALQRPDVLLPQVKAMIRASTAGRARIIFPMVS
                     APEELQAIKEVWERALDQIGPVAAPEWGIMVEVPSALFMMDEIARYTKYISLGTNDLL
                     QFVYGMDRTNERLNTLAHPLCLPFLRLLLYSVAAAKGEGITVGICGEMAGDPAGFVML
                     AGLGIDEFSMRPAALPTIWKMIPQLNRRDVVAWSYDLLARGARADVRLEMARAFPRLA
                     SIVCPD"
     gene            complement(2690..2965)
                     /locus_tag="PKO06_02140"
     CDS             complement(2690..2965)
                     /locus_tag="PKO06_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNV68468.1"
                     /translation="MKRQDVEIKNKFGLHARPASLLVKLATSFQSEVQLVKEDMEVNA
                     KSILGVMMLAAGPGNLITIVANGPDEEDAVKALAALVDSRFGEEEAA"
     gene            complement(2978..3769)
                     /locus_tag="PKO06_02145"
     CDS             complement(2978..3769)
                     /locus_tag="PKO06_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HNV68469.1"
                     /translation="MPELSRLTLLQVAFRSFFLQASWNFRGMMSMGFLYAIKPALDQI
                     YADPELRRQAYVRHMKYFNTNPYFSSILMGVVLALEERVARREITDDIIDDTKEGLMS
                     AFAAVGDGFFWDSWRPFVATFALLFAFNNFLLTPIIFLTLYNLPHLYLRFRGVFWGYR
                     MGTDVIRILGRFQLPRVRLGLRYATMVLLAYLVANHVNLHTPLLITNLPMEYFYWGEK
                     IVQGIGATLLVGLSALAYRSKIDVLTISFLLMLLALLLYHWGILI"
     gene            complement(3762..4556)
                     /locus_tag="PKO06_02150"
     CDS             complement(3762..4556)
                     /locus_tag="PKO06_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HNV68470.1"
                     /translation="MIEVWQAALLVAVIGGIVDLDSTATWQFMVSQPVVAAPLTGLFL
                     GYFHGDVVSGLRLGLMIGTILQLVWIEQLPLGMNIPPDAALASVLSVALGYLAGRYFE
                     SYVEREVCNTVALLIAVGLGLLGRSLDIWIRRLNTSIDNWVAGKLEAGAFHALDVGHS
                     LGGFLTFLKAFLFCFVAVLIGVEPLRTFTQSLNISQNTGFIVIEGLLPAVGFAVLASR
                     CLQTPRELQVFLLGVAAFTLLPAWFWLPVIFGLGIGWYSSRSPSNA"
     gene            complement(4549..6525)
                     /locus_tag="PKO06_02155"
     CDS             complement(4549..6525)
                     /locus_tag="PKO06_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1925 domain-containing protein"
                     /protein_id="HNV68471.1"
                     /translation="MSRNPLSLVLVLHAHQPISNPDEIVCEIIDRCYRPVLTTLSHHS
                     ALAFVLHVSGSLLQRWQKLAPDLIDLVKTGVRRGQIELLGGGWHEPLFPFIPAIDRQG
                     QIRELARRVKTLFGTRPRGVWLAERVWEPSFPVDLSAAGADYTLLDDHAFLQTGWAEH
                     QLTGPFLTEDQGCQIGIFPISRALRYLIPFADHDVLMNSLFERGQRNGGVWVYADDIE
                     KMGAWPGTYERMHEQGWLETFCELLLGAADWLRLERLDTVWARNEWAGLAYLPTTSYP
                     EFLRWSLPPYLRGRPGTSGSPRHFLVRYPEVRILQRRMLAASRAVHARSRPPAAWLDR
                     LWQSQTSCAYWHGWFGGVYDPVLRQSARMAAAQVEHEARAVTGVELSREDVDGCGAPE
                     LIVRTPAQWLALQPSDGAMIGWEASHTGFDLCGVMHAWDEEGRLETEDALPTHLKPLA
                     AFFLPAHFSGIPFRGDSDARVIPMILQAEPPRIDLYENELCIEIAWSLKLCLSEGDIP
                     LRLRKTVKIADKGCRFEVNWHLRNESGNPVETALGFWLPVSVHPSRPDWVPEPGFPEL
                     GVESVDDQIEGEAIGIQELQVGIPGAETLLIRAEQPYTAWYQPMIQRLMVEGRERSLL
                     QGLSLILTTSLHLEPRVDVSFGFELSRSSGTRDD"
     gene            complement(6522..>7199)
                     /locus_tag="PKO06_02160"
     CDS             complement(6522..>7199)
                     /locus_tag="PKO06_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV68472.1"
                     /translation="PDGWWFEPFLFGFGGSYFENDKLSFQSEATIKATHFLIDLKETA
                     HALPPVNLRTSSYDVMMTSFKNGQVSMVINGPWAIREAFAGQAFKENVSNLLIAPLPK
                     GPAGFYTPVGCQTWVVPSSCKNRKEAIELIKFLSSTEAMAVVCKKNYSIPARQSLFND
                     PELRKDPFLAPFLRQMQECRPHPNDHADRGELYTLIGEYLVKVLSGDVTPEDGLKDLT
                     AAWKGKK"
BASE COUNT         1594 a         2149 c         2181 g         1275 t
ORIGIN      
        1 gcggtggggg cggcggcgga ggaggaggtg gtggtggtgg agccacaggc ttcacttctg
       61 ctttcggcgg aggagccatg ggcttcggtt caggtctgac caccgaagca actttcggtt
      121 caaccggggg aaccgtcggc ggttccggtc tggcggcggg tttcgccggt tgcggcttca
      181 tggggggcag atcgggttcc cctagatcga gattgagttc tccgccactc gcgccaagat
      241 cgagaccgac gggttcctgc gtgtcgaggt tcagatccag agccccggaa tcggtggtat
      301 cgaggttcaa atcaagacca tcagccgcag gagcttcgat cgagccggta tcgagagcgg
      361 cgggttgtgc atgaagatca aaagaaaggt cgccttcggt gatcggctgc gattcgaggt
      421 tcaggccgcc ggcagaggca ataccggctt cggacgagcc gagatcaaga tcgagtcccg
      481 ccggcttcga cccgccatcc aggttgatgc ctccgagatc gagcccgcca tcggccgcgg
      541 gcgtgccaag atcgagattg agatcaagcc cggaggtctc gccgccggag gacggggaac
      601 cgtcacccag atcgagcgac agatcaagct cgagcggtgc tctttccatg ggagagccgg
      661 catcgccttc cgccggtaaa gccatcccac acatgttgca gaacttgttc ccaccttcgt
      721 tttcaaaacc acaagcgcag cgcatgtttc cctccgatat acgggctcag cctcagaaca
      781 gattattcaa tcgcaaagca taacagaaaa cagcaccatt gtctcacatg gtgcatcatt
      841 tcctttcatc ggcacatgac ccgttcaacg atagcactca ggcaatgtcg cgccagctct
      901 gccgacacag agctttttcg tcggtggaag agcgagggac tcgctcgcga acgttttgcg
      961 agacgagcga cgactcagtc ggggcagacg atcgacgcga gacggggaaa ggcacgggcc
     1021 atctcgagac gcacatcggc acgagcgcca cgagccagca gatcgtagga ccaggccacg
     1081 acatcgcgac gattcaactg gggaatcatc ttccagatgg tgggcaacgc ggccgggcgc
     1141 atgctgaatt cgtcaatacc aagaccagcc agcatcacaa accccgcggg gtcacccgcc
     1201 atctcgccgc agatcccgac ggtgatcccc tcccctttcg ccgcggcaac gctatacagc
     1261 agcaaccgca gaaacggcag acacagcggg tgcgccaggg tattcaggcg ttcattggtg
     1321 cggtccatgc cgtagacgaa ctgcagcaga tcgttggttc cgaggctgat gtatttggtg
     1381 tagcgggcga tttcatccat catgaagagc gcggaaggca cttcgaccat gatgccccat
     1441 tccggggctg ccaccgggcc gatctgatcc agggcgcgtt cccagacttc cttgatggcc
     1501 tggagttctt cgggcgctga gaccatggga aaaatgatgc gggcacgccc cgccgtcgac
     1561 gcgcgaatca tggcttttac ctgcgggagc aggacatccg ggcgctgcaa cgcgaaccga
     1621 atcgagcgaa accccatcgc cgggttgact tcgcgcggaa acggcaggta cttgactggt
     1681 ttgtccgacc cgatatcgat agtacgcaac accgccatgt cacgcgccgt ctcgaccacc
     1741 tggcggtaga tctgctcctg aaaagtctca gagggcaaat ccacgctttc catgaacagg
     1801 aactcactgc ggaacagccc gatgcggcga atgttctggc gttcgagcat ggccagttcc
     1861 tcaacccggg cgacattcgc tccgaccgtg aaccgatagc cgtcagccgt gaccaaccct
     1921 tcggcgggca tctggggagc ttctgccaca ggggcaatcg tcgccacctg ttccgcagcc
     1981 gggtgcagga cgaccaaacc ctgcgaacgg gtatcgacca ggatctggtc gcccgtatgc
     2041 gcgacccgcg agagatcggt gagaccgaag accgagggaa tgccgagcgc gccggcaaga
     2101 atcgccacat gagaggtggc accgccggaa tcaaggcaga tgccgcgcag atgccccttg
     2161 gcgaattcga gcagctcggt cggggtgaca tcgctggcca gcagcacgaa aggttccgcc
     2221 ccgatctcgg cggccggtcc ctcgaggccg agcagatgcc gcaggatccg atcgccgaca
     2281 tcctgcatat cgacggctcg ctcacgcaga tactcgtcct cgatcgcgag aaaacgctgc
     2341 agaaacgaat tgacgacgtc ctgcacgatc acttcgatgt tcagacggcg cttctgcacc
     2401 tgcttttcga cttcgggccg aaaggaagga tcggagagca tgagcagatg cgaatcaaag
     2461 atggcggcgt atttctgacc gtgcttgctc agggcttcct gttgcacggc gagaatgtca
     2521 cggcgggtga gttcgatcgc ttcggccaga cgctcgagtt cgcgcgcaac cgcggtttct
     2581 tcaatggggc ggcgttcgat ttcgaggcgg cggcggaaga cgtaggcgcg ggcaacgcag
     2641 gtgccaacgg agcccgtgat ccccaccagg cggaggggcg tcggccgggt catgccgctt
     2701 cctcttctcc gaagcgggaa tcgacgagtg ctgccagggc cttgaccgcg tcttcttcat
     2761 cagggccgtt ggccacgatg gtgatcaggt tcccgggccc ggccgccagc atcatgacgc
     2821 cgagaatgct tttggcattc acttccatgt cttctttgac cagctgcacc tcgctttgaa
     2881 aggaggtggc cagcttcacg agcagggaag cgggacgcgc atggagaccg aatttattct
     2941 tgatttcgac gtcttgccgt ttcatgaccg gtttaactta gatgaggatc ccccaatggt
     3001 acaacaagag ggccagtaac atcaacaaaa aagaaatggt caggacatcg attttcgaac
     3061 gatacgccaa tgcggaaagt ccgaccagca aggtcgcccc aattccctgc acgattttct
     3121 cgccccaata gaagtattcc atgggcaggt tcgtgatcag cagaggagtg tgcaggttca
     3181 cgtggttcgc gacgagatag gccagcagca ccatggtggc atagcgcagc ccgagtcgca
     3241 cgcgcggcaa ctggaagcgt ccgagaatgc gaatgacatc ggttcccatt cggtatcccc
     3301 aaaacacgcc tcgaaagcgc agatacagat gcggcagatt gtacaatgtc agaaagatga
     3361 tcggggtcag cagaaaatta ttgaacgcga agagcagagc aaacgtggcg acgaacgggc
     3421 gccaggaatc ccagaagaag ccatcgccca cggcggcgaa ggcactcatc aatccttcct
     3481 tggtgtcgtc gatgatgtcg tcggtgatct cccgccgcgc cacccgctct tcgagcgcca
     3541 gcaccacacc catcagaatc gaggaaaaat acgggttggt gttgaagtat ttcatgtgcc
     3601 ggacataggc ctgacgccgc agctcggggt cagcgtaaat ctgatccagc gccggcttga
     3661 tggcatagag aaaacccatg ctcatcatac cgcggaagtt ccaggaggcc tgcaggaaga
     3721 aactgcgaaa cgccacctgc agcagtgtca gacgactgag ttcaggcatt ggacggagag
     3781 cggctactgt accagccgat accgaggcca aagatgaccg gaagccagaa ccaggctggt
     3841 aacaaggtaa aagcggcaac gcccagcagg aacacctgca actcacgggg agtctgcaga
     3901 cagcggctgg ccagcaccgc aaaaccaacc gccggcagca ggccctcgat gacgatgaag
     3961 cccgtgttct ggctgatatt gagtgactgc gtgaaggtgc gcaaaggttc gacgccgatc
     4021 agaacggcga caaaacagaa gagaaacgcc ttgagaaagg tgaggaaccc accgagcgag
     4081 tgcccgacat cgagcgcatg aaacgccccg gcttcgagtt tccccgccac ccagttgtcg
     4141 atcgaggtgt tgaggcgacg gatccagatg tcgagcgagc ggccgagcag acccagaccg
     4201 acggcgatca gcaaagccac ggtgttgcac acttcgcgtt cgacatacga ctcaaagtac
     4261 cggccggcaa gatagccgag cgcgacggaa agcacggaag cgagcgcggc atctggcgga
     4321 atattcatgc ccagcgggag ttgttcgatc cagaccagtt gcaggatcgt gccgatcatg
     4381 agccccagcc gcaggccaga gacgacatcg ccatggaaat acccgagaaa caacccggtc
     4441 aggggcgcgg caaccaccgg ttgcgacacc atgaactgcc aggtggccgt cgagtccagg
     4501 tctacgattc caccgatcac cgctaccagc agggctgcct gccagacttc aatcatcccg
     4561 ggtgccgctg gaccgtgaca gctcgaagcc gaaggacaca tcgacccgcg gttcgaggtg
     4621 cagagacgtg gtgagaatca gcgagagccc ctgaagcagg gagcgctcgc gcccttcgac
     4681 catcaaacgc tgaatcatcg gctgatacca tgccgtgtat ggctgttccg cgcggatcaa
     4741 aagagtttcg gcacctggaa ttccaacctg gagttcctga attccaatcg cctcaccctc
     4801 gatctggtca tcgacacttt caacacccag ctccgggaaa cccggttcag gcacccagtc
     4861 cgggcgggag ggatgaaccg ataccggcag ccagaagcca agcgcggtct cgacgggatt
     4921 gcccgactcg ttccgcaggt gccagttcac ctcgaacctg caccctttat cggcaatttt
     4981 caccgttttc cgcagccgca gcggaatatc cccctcggac aggcacagtt tcagcgacca
     5041 ggcaatctcg atgcagagtt cgttctcgta caggtcgatt cggggcggtt ctgcctgcag
     5101 aatcatcgga atcacccgcg catcggaatc gccccgaaac ggaatgccac tgaagtgagc
     5161 gggcaggaag aaggccgcca ggggcttgag atgtgtcggc agggcgtctt ccgtttcgag
     5221 gcgcccttct tcgtcccacg catgcatgac gccacacaga tcgaagccgg tgtgggatgc
     5281 ttcccagcca atcatggcac catcagaggg ttggagcgcc agccactgag ctggagtgcg
     5341 aacgatcagt tcgggggcgc cgcagccatc gacatcttcg cgactgagtt cgacgcccgt
     5401 caccgctcgg gcttcgtgct ccacctgagc ggcggccatg cgcgcactct gtcgcagcac
     5461 cggatcatag accccgccaa accagccgtg ccagtaggcg cacgacgtct gagactgcca
     5521 gagacgatca agccacgccg cgggtggacg ggaacgcgcg tgcacggcgc ggctcgcggc
     5581 caacatgcgg cgctgcagga tgcgcacttc gggataacgc accaggaagt gcctgggact
     5641 accggacgtg cccggtcgac cccggagata cggcgggagt gaccaacgca gaaactcggg
     5701 ataggaggtc gtcggcagat acgccagacc ggcccactcg tttcgcgccc agacggtatc
     5761 gagccgttcg agacgcaacc agtcggccgc tcccagcagc aactcgcaga acgtctcgag
     5821 ccagccctgt tcgtgcatgc gctcgtaggt tccgggccag gcgcccatct tttcgatatc
     5881 gtccgcgtag acccagactc ccccattgcg ctgtccgcgc tcgaacaggc tgttcatgag
     5941 cacatcgtgg tcggcgaagg gaatcagata ccgcagcgcc cgagagatcg gaaagatacc
     6001 gatctgacac ccctggtctt cggtcagaaa cggccccgtc agctgatgtt cggcccagcc
     6061 ggtctgcaga aaagcgtggt cgtcgagcaa ggtgtagtcc gcgccggcgg cagaaagatc
     6121 gaccgggaat gagggttccc agacgcgctc ggcaagccag accccccggg gacgtgtccc
     6181 gaagagggtc ttcacacggc gcgccagttc cctgatctgc ccctggcgat cgattgccgg
     6241 aataaagggg aacaagggtt cgtgccagcc accgccgaga agctcgatct gtccacggcg
     6301 tacgccggtc ttcacgagat cgatcaggtc gggagcgagt ttctgccagc gttgcagcag
     6361 tgaccccgac acgtgcagaa cgaaagcaag cgcggagtga tgcgaaagcg tcgtcagaac
     6421 cgggcgataa cagcgatcga tgatctcgca cacaatctca tccgggttgc tgatcggttg
     6481 gtgggcatgc agaaccagaa ccagggaaag cgggttccga ctcacttttt tcctttccag
     6541 gcggcggtca ggtctttgag accgtcttcg ggtgtcacat cgccgctgag caccttgacg
     6601 aggtactccc cgataagtgt gtacaactca ccgcgatcgg catgatcgtt tggatgcggc
     6661 cgacactcct gcatctgtcg taaaaacggt gcgaggaagg ggtctttccg cagttcagga
     6721 tcgttgaaca gtgactggcg tgcgggaatc gagtagttct tcttgcagac caccgccatg
     6781 gcttcggtcg acgacagaaa cttgatcagt tcaatcgctt ccttgcggtt cttgcaggaa
     6841 gacgggacga cccacgtctg acaaccgacc ggggtgtaaa agcccgcagg tcctttcggc
     6901 agaggggcga tgagcaggtt gctgacgttt tctttgaacg cctgaccggc aaaggcttcg
     6961 cgaatcgccc atggcccatt gatgaccatg ctgacctgcc cgttcttgaa ggaggtcatc
     7021 atgacatcgt aggaactggt acggagattg accggcggca gcgcatgcgc tgtttccttc
     7081 aaatcgatca agaagtgtgt cgccttgatc gtagcttcgc tctggaagct gagtttgtca
     7141 ttttcgaagt aggaccctcc gaagccaaat aagaacggtt cgaaccacca gccatcggg
//
LOCUS       DAORHD010000056         7141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10257_length_7141_cov_3.667513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000056 DAORHD010000000
VERSION     DAORHD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_7141_cov_3.667513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKO06_02165"
     CDS             <1..250
                     /locus_tag="PKO06_02165"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68473.1"
                     /translation="GDAIIFYTDGYIEALNETGVMFGYDRFRDVFSRYAHQSATRCID
                     DMLSFHRSFLGAGSAGDDVTLLVLKRLTGTSPISESHP"
     gene            247..639
                     /locus_tag="PKO06_02170"
     CDS             247..639
                     /locus_tag="PKO06_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68474.1"
                     /translation="MKVSNPLIILIALVSCLLLVFGPTYLDHLKSAQEARQQEELEKL
                     REQRSGSSSHRPDDFADTELVAEDDPLIAAEEGLIPEDEEVLTGDGAETATQPRTPQA
                     SSSSRPASDIPAASFSRSFSESATKSPH"
     gene            678..920
                     /locus_tag="PKO06_02175"
     CDS             678..920
                     /locus_tag="PKO06_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68475.1"
                     /translation="MMHPVIVPRHIAIVMAHDWKLAREKHQRMLLHTTASAPPSAEPL
                     RPPLFMSASQAKALAAEWELFRRAAEEKPGKQKTGV"
     gene            complement(948..1250)
                     /locus_tag="PKO06_02180"
     CDS             complement(948..1250)
                     /locus_tag="PKO06_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68476.1"
                     /translation="MFFKHILVGFIILGILGFLYGDHVLHFQANLMIQWMYDFPAYEA
                     YERIVRYYPNSSFKTEAKKMMDILIKRNRDLAKYLETRDKETRALEKKRAQNESFH"
     gene            complement(1298..2239)
                     /locus_tag="PKO06_02185"
     CDS             complement(1298..2239)
                     /locus_tag="PKO06_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68477.1"
                     /translation="MENNKIQELVFSFMDSVGAKPVQAGEGIWQAPIPSAETGFFNGD
                     KDFRFTFSRDVAEKHRDLELICEGSYLLKRIIDRLSTVPKVSRLFLKAQPTLPPLVSG
                     KTPELRVITPGKVYYRQQVWFNFRVKYECDRKEEMFFSTLADPALPQVELHEQLSKVD
                     ETLYSEEPEPGVPIEDLGEDLLRLYLSSCRAVEEKIADQIQDVRDWAEEKYKHEEQRF
                     IAYLEEQKRELQKKKENVCFHLYFFQKEEEIERMIKDLETEKNRKLAELKEKFAVQVE
                     VALINAVIIAVPTLGVPAGKITRKLREGAGNRVAFSG"
     gene            complement(2226..4334)
                     /locus_tag="PKO06_02190"
     CDS             complement(2226..4334)
                     /locus_tag="PKO06_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNV68478.1"
                     /translation="MKSALIKVTNVNAHSFTFQLKGKAFKEGYLFLTPEMATLFPFRE
                     ELTSVTFLDEADVVHSAIYHKSRGYMVCLTFGEWWERHHPSDGDLVAIEVIDPAHRQY
                     RLRHLGNRPAPRVSQEEKISLTVRPAGDAAIVYPDANLEVLRKLLRGDISPWRETALI
                     LKGEEMNRSGDFEDLIALRSSFGVAAYEHQLNTVETVIHRMNGRALLADEVGLGKTIE
                     AGLALKEYLLRGMVKKVLILTPASLITQWKQELKTKFDLNFKSHSEVSDWGASDLIIA
                     SLDTAKSQKNAKSVQQINYDLLIVDEAHKLKNKKTQNWKFVNSIKTKYLLMLTATPIQ
                     NDLLELFNLISILKPGHLSTVQKFKHEYLEDRDKRIPKNSVALRSMLSEIMIRHRRSD
                     TLIKFPQRFVHTHMLHLNDDETKLYAELTEFIRSRYFTLPTHKNPRGINRLTLILLQK
                     LMGSSTHALQRALEKMISSNFYKDDFDQNLRKLLDMAKSVKNSRKSQALLDIMKPLDD
                     KMIIFTQFRGTQDFLKRLLRENGYTVSVFNGQLSQSEKDECIEEFRNKTRVLISTESG
                     GEGRNLQFCKALVNYDLPWNPMRIEQRIGRIHRLGQLEDVHIINLSAEDTIESYVLEL
                     LDKKINLFRLIIGELEMILGNLHTKKSLEQIIIDIVTMSPNREAMKRKFQTLGNKLER
                     AYQTHQDITQAQEEIFHGEQ"
     gene            complement(4365..5642)
                     /locus_tag="PKO06_02195"
     CDS             complement(4365..5642)
                     /locus_tag="PKO06_02195"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV68479.1"
                     /translation="MRQTIRSLGFAGMLCSAAGAACAEPVVAGHHLAEARAGASALFL
                     AHVFNADQKPLKAGNYEIGLELSGGQGRKVYTLKPTTDIDAGRISTFRMPLNGAHTAS
                     TNGNFRVFVRAGKQTTFSESYTLTNHRLGGAVHGKHTALYTEAPPETGDSGRPPAEVP
                     FEDELVKLDAKGVTSAKTTGKPVTAKPATTRPTAVKPHAVTTSTTGVPTPTSAAAISL
                     PVNAPKTVSAKQPGTTPVAKPAAKEPTVAKKETVTTVAVTPESPAKPRQIDASEFKTI
                     RTIDEELIIYVIRTGDTLKSIAERYYGSAAKERVIAEFNFIDDPKSIKVGEEIIVEVK
                     PLKQGANVAPTQSHSSEAAAKPQSATKSDVAAASGATYVIQAGDNLGKIAKQTLGKAT
                     LASKLLEANPGLNPKNLKVGTTILIPEISGKQG"
     gene            complement(5965..>7141)
                     /locus_tag="PKO06_02200"
     CDS             complement(5965..>7141)
                     /locus_tag="PKO06_02200"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV68480.1"
                     /translation="FAELLAYLGMGQSPRGALHIFFTRGWVFFSMGMFSSYVMYLCSQ
                     PAKGEFTVVTCLGLGLEIFYCWQHGGIGSGLGCAALLALFLNALATDGKAGQLFLQRF
                     LSALVPPFFILLTPTFLDATVKLNPSTLDHFAYAYDRCLGGQLSFFLARLFAQVFWFE
                     KASMFFYVTLPLAWALVYVSGLNKRGAIALRVFHSFIISAVIANIVFHLFPCGGPKNF
                     FGTLFPNTLPVVEALVSQVMSALTPRNAVPSLHTTWVLLLWWNSRSQSLKTRTVTYLY
                     LAFTLFATLGLGEHYLFDLIVAIPFAVSVQALCFYELPLTDNRRSRPLVVGILLFFLG
                     LLFARFGLASVEPCSSLVWLVTLVLVLIPLYLESRLWQAAEEFGGMISPASTVVSNQ"
BASE COUNT         1568 a         1999 c         1951 g         1623 t
ORIGIN      
        1 cggggacgcc atcatcttct acaccgacgg ctacatcgaa gccctgaacg agacaggcgt
       61 catgttcggc tacgacaggt ttcgtgatgt attcagccgg tacgctcacc aatcggcaac
      121 tcgttgcatc gacgatatgc tgagttttca ccgttcgttt cttggcgctg ggtcagctgg
      181 tgatgacgtc actctgctgg tactcaagcg gctcactggc acctcaccca tatcggagtc
      241 tcacccatga aggtatcgaa ccctctgatc attctgattg cgcttgtgtc ctgcctgctt
      301 ctcgtgttcg gtccgaccta tctcgatcac ctcaagtccg cgcaggaggc tcgtcagcag
      361 gaggagttgg aaaagttacg ggaacaacgg agcggatcct cgtctcatcg gcccgatgac
      421 ttcgccgaca ccgagctcgt cgccgaggac gatcccctca ttgcggcaga agaagggttg
      481 attcccgaag atgaggaagt cctgaccgga gacggtgcgg aaacagcaac gcagccccgg
      541 actccgcagg cttcctcttc atcgagaccg gcctctgaca tccctgcggc gtcattctca
      601 cgatcatttt ctgagtctgc cacgaagtct ccgcactgac tccaccattc ggcccgccga
      661 tcacggttcc gtttcccatg atgcacccgg tgatcgtccc ccggcacatt gccatcgtga
      721 tggctcacga ctggaaactc gctcgggaga agcatcaaag aatgcttctg cacacgacag
      781 ccagcgcgcc accgtccgcg gagccacttc gaccaccgct gttcatgagc gcttctcagg
      841 ccaaagcgct ggccgccgag tgggaattgt tccgccgcgc ggccgaagag aagcctggaa
      901 aacaaaaaac cggggtgtga cccccggttg tctgccatgt gcctgcttta atgaaatgat
      961 tcgttctgag ctctcttttt ctcgagcgct cgcgtttcct tgtcgcgggt ctcgagatac
     1021 ttggccagat cccgattccg tttgatcaga atatccatca tttttttcgc ctcggtcttg
     1081 aacgagctgt tcggatagta gcgaacaatg cgttcatacg cctcgtaggc aggaaaatcg
     1141 tacatccatt gaatcatcag gttggcctga aaatgcagca cgtgatcgcc atacagaaac
     1201 ccaagaatgc ccagaatgat gaagccgacc aggatgtgtt tgaagaacat cgcgcaccct
     1261 cctcagaata tcccttccac ttctaaagat atcctgttca gccggaaaac gccactcgat
     1321 tgccagcccc ttctcgaagt ttgcgcgtaa tctttccggc tggcactccc agcgtcggaa
     1381 ccgcgatgat gaccgcattg atcagagcga cctcgacctg aacggcaaac ttctctttca
     1441 gttccgccag tttccgattc ttttcggttt ccaggtcctt gatcatgcgc tcgatctctt
     1501 cttctttctg aaagaagtac aggtgaaagc agacattttc cttctttttc tgcagttcac
     1561 gtttctgttc ctcgagatac gcgatgaacc gctgctcttc gtgtttgtat ttctcttcag
     1621 cccagtcacg cacatcctgt atctggtcgg caatcttctc ttcgaccgct cgacacgaag
     1681 acagatagag acgcagcaga tcttcgccga gatcctcgat gggcacgccc ggctcgggtt
     1741 cttccgaata gagagtctcg tcgaccttcg acagctgttc atgcagctca acctgcggca
     1801 atgccgggtc ggccagtgtg ctgaagaaca tttcttcctt gcggtcgcac tcgtacttca
     1861 cccgaaaatt gaaccacacc tgttggcgat agtacacctt gccgggggtg atcacccgca
     1921 actcaggcgt ctttcccgac accagaggcg gcagggtagg ctgcgccttg aggaacagac
     1981 gactgacctt gggcaccgtc gacagacgat cgatgatgcg tttgagcagg tatgatcctt
     2041 cgcagatcag ctcaagatcg cggtgtttct ctgcgacatc acgtgagaac gtaaagcgaa
     2101 agtccttgtc gccgttgaaa aaaccggttt cggcgctcgg aatcggtgcc tgccaaatac
     2161 cttcaccggc ctgcaccggc ttggccccga cggaatccat gaagctgaag acgagttcct
     2221 gaattttatt gttctccatg gaaaatttcc tcctgagcct gagtaatgtc ctgatgcgtc
     2281 tgataggcgc gttcgagttt gttgccgagg gtctggaact tgcgtttcat ggcctcgcgg
     2341 ttgggtgaca tggtgacgat gtcgatgatg atctgctcga gactcttctt ggtgtgcaga
     2401 ttgccgagaa tcatttcgag ttcgccaatg atcagcctga acagattgat cttcttatcg
     2461 agcagttcga gcacgtagga ttcgatcgta tcttcggcgc tcaaattgat gatgtgcacg
     2521 tcttccagct gaccaaggcg gtgaatgcgg ccgatgcgct gttcgatccg catcggattc
     2581 cacggcagat catagttcac gagggctttg cagaactgca ggttgcgacc ttcaccgccc
     2641 gattcggtcg agatcagcac gcgggtcttg ttccggaact cctcgatgca ctcgtccttt
     2701 tcagactgcg acagttggcc gttgaagacg gaaacggtat acccgttctc ccgcagcagg
     2761 cgcttcagaa agtcctgggt gccacggaac tgcgtgaaga tgatcatctt gtcgtcgagc
     2821 ggcttcatga tgtcgagcag ggcctgcgac ttgcgtgagt ttttcacgct cttggccata
     2881 tcgagcagct tgcgcagatt ctgatcgaag tcgtccttat agaagttgct gctgatcatc
     2941 ttttcgagcg cgcgctgcaa cgcgtgtgtc gaactgccca tcagcttctg caacagaatc
     3001 agggtcagac gattgattcc gcgggggttc ttgtgggtcg gcagggtaaa gtaccggctg
     3061 cgaatgaatt cggtcagctc tgcatacagt tttgtctcat cgtcgttcag atgcaacata
     3121 tgcgtatgaa cgaatcgctg tggaaacttg atcagcgtat ctgagcgccg gtggcggatc
     3181 atgatctctg acaacatgga ccgaagagcg accgagtttt tgggaatgcg cttgtcgcga
     3241 tcctccagat actcgtgctt gaacttctgg acagtcgaca gatgaccggg cttcagaatc
     3301 gatatcagat tgaacagttc aagaagatcg ttctgaatgg gcgtcgcggt gagcatgagc
     3361 aggtacttcg tcttgatcga attgacgaac ttccagttct gggtcttctt gttcttgagc
     3421 ttgtgggctt cgtcgacgat caacagatcg taattgatct gctgcacgga cttagcgttc
     3481 ttctgactct tggccgtatc gagagaggca atgatcaggt ccgaagcgcc ccagtcgctg
     3541 acttcgctgt gactcttgaa gttcagatcg aacttggtct tcagttcctg cttccactgc
     3601 gtgatcagcg aagccggagt cagaatcagc accttcttga ccatgccgcg caacaggtac
     3661 tctttcagcg ccagaccggc ttcgatcgtc ttgccgagac cgacttcatc ggccagcagg
     3721 gctcggccgt tcatgcgatg aatcaccgtc tcgaccgtat tcaactgatg ttcataggca
     3781 gccacgccga agctgctgcg cagggcaatg agatcttcga agtcgccgga gcggttcatc
     3841 tcttcgccct tcaaaatcaa cgcggtttca cgccagggag agatatcgcc gcgcaacagc
     3901 ttgcgcagca cttcgaggtt cgcatccggg tagacgatcg ccgcgtcacc cgcgggtcgc
     3961 acggtaagac tgatcttttc ttcctggctc actctgggtg ccgggcggtt tcccagatga
     4021 cgcaaccggt actgacgatg tgcggggtca atgacctcga tcgccaccag gtcaccatcg
     4081 ctcggatgat ggcgttccca ccactcgccg aacgtcaggc agaccatgta accccgcgat
     4141 ttatgataga tcgcggaatg cactacatcg gcttcgtcga gaaacgtcac cgaggtcagt
     4201 tcctcgcgaa aggggaaaag agtcgccatt tcgggggtga gaaacagata cccttccttg
     4261 aaggccttgc ccttcagttg aaaggtaaag ctgtgcgcgt tgacattcgt aaccttgatc
     4321 aatgcagatt tcactttttt gctcccatct tccacggcgc ttctttaacc ctgctttcca
     4381 gagatttcgg gaatcaggat ggtggttccg accttgagat tcttcggatt caatccgggg
     4441 ttcgcttcca gcagcttcga agccagagta gccttgccaa gcgtctgttt ggcaatcttt
     4501 cccaggttgt cgccggcctg aatcacatac gtggcaccag aagcagcggc aacgtcagac
     4561 ttcgtggcac tctggggctt cgccgcggcc tcactggaat gactctgcgt gggagccaca
     4621 tttgcgccct gtttcagggg tttcacctcg acgatgatct cttcaccgac tttgatcgac
     4681 ttcggatcat cgatgaagtt gaattcggca atgacccgtt ccttggcagc cgaaccgtag
     4741 tagcgttcag caatgctttt cagggtatcg cccgtccgaa tgacataaat gatcagttct
     4801 tcatcgatcg tgcgaatggt cttgaattcg ctcgcatcaa tctggcgcgg tttggccggc
     4861 gactcgggcg tcacggcaac cgtcgttacc gtctctttct tggccacggt cggttctttc
     4921 gccgccggct tggcaaccgg agtggtcccc ggttgtttcg cggaaaccgt tttcggcgcg
     4981 ttcaccggaa gggagatagc tgcagccgac gtgggtgtcg gaacacccgt cgtggaagtc
     5041 gtgaccgcat gaggcttgac ggccgtcgga cgcgtcgtgg cgggctttgc cgtcaccggc
     5101 ttgccggtcg tcttcgccga ggtcacgccc ttcgcatcga gcttcaccaa ttcgtcctcg
     5161 aatggtacct cggccggcgg gcggcccgaa tcgccagttt caggcggtgc ttcggtatac
     5221 agagcggtgt gtttcccatg caccgcaccg ccgagccgat gattggtcag cgtgtaagat
     5281 tccgaaaacg tcgtctgctt gcccgcgcgg acgaaaacgc gaaagtttcc gttcgtggat
     5341 gcggtatgcg cgccgttcaa cggcatgcgg aacgtgctga tgcgaccggc atcgatatcg
     5401 gttgtcggct tcagagtgta gacttttcgg ccctgcccgc ccgaaagttc aagcccgatc
     5461 tcgtaattcc ctgccttcaa aggcttctga tcggcattga acacatgggc gagaaagagg
     5521 gcactggcgc cagcccgcgc ttcggcaaga tgatgaccgg ccacgacggg ctcggcgcag
     5581 gcggctccgg cagcggaaca cagcataccc gcaaagccga gtgaacgaat cgtttgcctc
     5641 ataactgacg gttctcctgg gaaacgtttg cttttctgtt agaactatcg gcagtttgcg
     5701 cccccggact gagcgccccg gaacatttcc ttgaaccgcc catcaagcct gtctccgaag
     5761 ccgcacgtgc agagattcct cacgtttcct gaggcacatc tgaaattatt gcgggcgacg
     5821 gatcggcgaa atctcactcg tcgaacgcta tcatgggttc tgcacgccat cgtgtcgcgg
     5881 gaggacgtcg tgatcccgga ctcttcgttt ctccgactgc cactcaccga aaatcgacaa
     5941 tgagttcctc agtcttgcgt gaattcactg attcgaaacg acagtcgacg ctggagaaat
     6001 cattccgccg aactcttctg ctgcctgcca cagacgagat tcgagataca gtggaatgag
     6061 caccaggacg agggtcacaa gccagacaag agacgaacac ggttcgacag aagcgagccc
     6121 aaaacgggca aacagaagcc cgagaaaaaa cagcagaatg cccacgacaa ggggccttga
     6181 ccggcggtta tcggtcagag gaagttcgta gaaacagaga gcctgcacac ttacggcaaa
     6241 cggaatggca acgatcagat cgaacagata atgttccccg agcccgagag tggcaaacaa
     6301 ggtgaacgcc agatacagat acgtcaccgt gcgagtcttc agcgactgag agcgtgagtt
     6361 ccaccagaga agcagcaccc aggtggtgtg cagtgacggc acggcgtttc tgggagtgag
     6421 agcactcatg acctgtgaaa ccagcgcttc gacaaccggg agcgtgttgg gaaagagcgt
     6481 tccgaaaaag tttttgggac caccgcaggg gaaaaggtga aacacgatgt tggctatcac
     6541 ggcggaaatg atgaagctgt gaaagactcg cagcgcaatc gcgccacgct tgttgagccc
     6601 tgagacatag accagcgccc aggcaagcgg cagcgtgaca taaaagaaca tcgaggcctt
     6661 ctcgaaccag aaaacctgag cgaagagtcg agcaagaaag aagctcagct gtcccccaag
     6721 acaccgatcg tatgcgtatg caaagtggtc aagggtcgag ggattgagtt tgacggtggc
     6781 gtcaagaaac gttggagtca acaaaatgaa aaatggcggg accaatgcag acaggaatcg
     6841 ctgtaaaaag agttgtccgg ctttgccatc ggtcgcaaga gcgttcagga acagcgccag
     6901 aagcgcggca cagcccaacc cactgccaat accgccgtgc tgccagcaat agaaaatctc
     6961 gagaccaagc ccgagacagg tcaccacggt gaattctcct ttggccggtt gtgaacagag
     7021 gtacatgaca taggaggaga acatgcccat ggaaaaaaag acccatcctc tggtgaagaa
     7081 gatgtgcaga gctccccgcg gagactggcc cataccgaga taagccagga gttcggcgaa
     7141 t
//
LOCUS       DAORHD010000057         7099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10355_length_7099_cov_4.337876, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000057 DAORHD010000000
VERSION     DAORHD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10355_length_7099_cov_4.337876"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKO06_02205"
     CDS             complement(<1..267)
                     /locus_tag="PKO06_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68481.1"
                     /translation="MDKIIVLTKTVTCGILEAYGLFARKKGLESWLAPKANVAPQVGG
                     AYELFWDLNDLENDSTIGCCLTGFEPKRFLSFEWRSPRQFKHFAN"
     gene            complement(340..2340)
                     /locus_tag="PKO06_02210"
     CDS             complement(340..2340)
                     /locus_tag="PKO06_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68482.1"
                     /translation="MIKSRSLALAALVSFGVSCCSVVQAQNPYSGDAPVQTQKTTANQ
                     TSRSGAPVTTLTPTIGEVAKNNGYATSEYAGYLVGYPIVQATIVFVDETKGYERFTWF
                     EQILNEHGCKKFKLDRKETVTGNIFNRKRSLTVTAQIEGRGFVVKAIKGHYGGTITGN
                     PDGVNSSGDADLKDVVNLELQNQQAELKILQYSFELDRLIIERSKLGALDFSGKNRVD
                     KAIKDLRENKIPAEKAKLQANHDTIGRLFNDRVTKAMTAKDYNRAIELIKASRTNTEA
                     SKYQLGNCYLGLGQHDQAIGYYKGLASSGTYGEKAELGMAMAYHAKGNDREALNAIYR
                     NLANFRNSADEVAALAKIDEWKLMSKTADFPELPEKISSVYVQKGLLNNQANHSTAVN
                     DYKRAAEIKANGGSAAAASKQILDEYAGVKSQYANQLANAKQTADNRFMNERERAKGS
                     YDAWQRSHNLAVSRANDEYYYEVQRKRRDLEEARRELDYLTRNPPKPSTGSGNGSSTD
                     PYSGGTKPNSGRSTTDPYGGRSGTTSRTGTDPYAGGNSSGNSGSGTDPYAGGNNGNGQ
                     GTDPYANEYQTRLEAARSKVTRLERDYNWLYYNQTAYVDDKTSSERASLNSARREYEK
                     YDLSKKPAYIAADSEVKRYTELTSAADSRFNALKQLAKEAGY"
     gene            2518..3723
                     /locus_tag="PKO06_02215"
     CDS             2518..3723
                     /locus_tag="PKO06_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68483.1"
                     /translation="MTRKLLLGSLFVLLLAGAVFANGSYELGQAKAAMDRGDFRYALD
                     QFRWITGNTSFGPEVRREAMYFIGFVYVKMSDPWSAIDSFRRFLDKYGSSSNEYLLPD
                     AMYVLGRTYEEVGRHDDARRLYRRCRERFPYNEFARKSEDRLRILGDSYYPPNPPPYP
                     PNPPYPPSYGITPEVRDMIELAKMSPDSYSADQMLLKACNRAQLGADFVALSKATRNQ
                     YTQYQIFDLCQKSRVYQMMTPYEVVDLAKTTTNSYYRNQLLLDYANKRARYAEDFRVL
                     MDACTDHFTKTQIMQIAQQKLGNGPMFNISMSVEAAPVKTSKKTETRVADTKVKAQKS
                     ASDPFVGFQSDAEKIRRVTWFLDAVQYKKGIDETSKLLKKEDMTLDVVRQAVKERESM
                     KKFDKLHGK"
     gene            3730..4506
                     /locus_tag="PKO06_02220"
     CDS             3730..4506
                     /locus_tag="PKO06_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68484.1"
                     /translation="MISRTGKAFLAGAFFFRSLNMNQHLFLLLVISLCAAPVFAANDD
                     TEIITIGPSVATPRQPTPATPARESPPAELSTLRTRAEAGNPDDQLTLGLALLDKRFG
                     VDDPVEGAIWITKAASQNHFEARTVLGRLKRDGRGVPRNYFEAVRHFRAAADAGYQKA
                     RIELGMMYALGQGVHQDALEAYTLLCLASPTLQLAPANELHAERAMLAKLEKQLSTDQ
                     RELALERMNDARDQSLSELMKRHPGKQGLPPAPMTDPVKK"
     gene            complement(4555..5793)
                     /gene="purD"
                     /locus_tag="PKO06_02225"
     CDS             complement(4555..5793)
                     /gene="purD"
                     /locus_tag="PKO06_02225"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNV68485.1"
                     /translation="MNILILGAGAREHAFAYGLSRSPTVHKIYACPGNAGMKEFCTRL
                     PFSSNSDLINVVKDRVELAVVGSPKYIELGTVDALTRAGIPVIGPAADAGRIETSKAF
                     AAKFIARHNIPAPFTHVVANRAEAEAFIHENPWVQVVKCDGFAAGTGVAVVDNPDEAI
                     GAVHRLLKTHGPPLILQERLQGLECSFAILTDGQQWISFSSCRDYKKIGEHDEGPTTG
                     GMGAVSPCPGLTREVEAEIQRRIVEPTVAGLRMDKLTYHGFLSFQLMLTTRGPKVLEF
                     NARLGDPEAQSILARFRGNLADLLLDCARGRLESLGSEVAFGRHFAVSVVLARQGYPL
                     DESAQPRVAGFESIRESTITHSGSEIDPGSTSYRFGGGRLMTLTALGDTLEQARNTCY
                     DDIRRLDLTNVIYRRDIGSA"
     gene            5943..>7099
                     /locus_tag="PKO06_02230"
     CDS             5943..>7099
                     /locus_tag="PKO06_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68486.1"
                     /translation="MKVTRLFSVSVGLFFLLLAGVTSGISQTLYSPAVTDLFNRPGSS
                     SPRDLYKMAHEHFTAGKLDDARLLAMRILLDGHRSQNLLDLLGTIEVKAGRPLLAGEW
                     LRKAISLNLEDNVARKVLLRLPPPPRPIPMDQGKLEAHFAQISQKLPLLLERLNSPSL
                     HFDSVLDEIARGQFYKALALAEEYEKKSPGANGTGLTALCALYLGRTRDALQLVEQGL
                     KLDPYHSLLLFVKVLGTDTHPDTSAPNRARALFDQDQWDEALKACDQLTALFPRSGDG
                     LLIKARIAYERRQYGQARSLADQASIRDPDHPGIDLLRSDIALAQNQREQAAQHIERA
                     FRRGYHLPSVTLKAAMLALLSEQSDEAARVLDETEALQPFVDRDAYPLFVEL"
BASE COUNT         1440 a         2173 c         1961 g         1525 t
ORIGIN      
        1 attggcgaaa tgcttgaact ggcgggggct gcgccactcg aacgagagaa agcgtttggg
       61 ttcgaaaccg gtcaagcaac agccgatggt gctgtcgttc tcgagatcgt tcaggtccca
      121 gaacaactcg taggcaccac caacttgtgg ggcaacattt gccttcggcg ccagccagct
      181 ttccaggccc tttttccgcg caaacaaccc gtacgcttcc agaattccgc atgtcacggt
      241 cttggtcagc acgataatct tatccatcgt ttatccctga aagagaagcc ggccaggaag
      301 gagacttccc ggccggctgg tggttctgtc aggtccggct cagtatccgg cttccttggc
      361 aagctgtttc aacgcattga aacgactgtc agccgcgctc gtcagctcgg tgtaacgctt
      421 cacttcgcta tcagcggcaa tgtaagcggg tttcttggag agatcgtact tttcatactc
      481 gcggcgggcg ctgttcaacg aagctcgctc gctcgacgtc ttgtcatcga catacgcggt
      541 ctggttgtaa tacagccagt tgtagtcgcg ttcgagccgc gtcaccttgc tgcgtgccgc
      601 ttcgagtctg gtctgatact cgttggcata ggggtcggtg ccttgcccgt tcccattgtt
      661 gcccccggca tagggatcag tgccagaacc tgagtttcca ctggagttcc cacccgcata
      721 cgggtcggta cccgttcgcg aggttgtccc tgagcgtcca ccgtaaggat ccgtcgtgga
      781 cctacccgaa ttcggcttcg tgccgccgct gtacggatcg gttgaagacc cgttgcccga
      841 gccggtgctg ggcttcggtg ggttgcgggt gaggtaatca agctcacggc gggcttcttc
      901 gagatcgcgg cgcttgcgct gcacctcata gtagtattca tcgttcgcgc gcgacacagc
      961 cagattgtga ctgcgctgcc aggcatcgta actgcctttg gcgcgctcac gttcattcat
     1021 gaaacggttg tctgccgtct gtttcgcatt cgccagctgg ttcgcatact gcgacttcac
     1081 accggcgtac tcatcgagaa tctgcttcga agccgctgcg gcgctgccac cgttggcctt
     1141 gatctctgcc gcccgcttgt aatcattgac cgcagtgctg tggttcgcct gattgttcaa
     1201 caggcccttc tgcacataaa cactcgagat cttctcgggc agttcgggga agtcagccgt
     1261 cttgctcatc agcttccatt catcgatctt cgccagagcc gcaacttcgt cagcggaatt
     1321 gcggaagttg gccaggttgc gatagatcgc gtttaaggct tcgcggtcgt tccccttggc
     1381 atgataggcc atggccatgc cgagctcggc tttctcgcca taggtgccgg atgacgcgag
     1441 acccttgtaa tacccgatag cctgatcatg ctgccccagg ccgagatagc agtttccgag
     1501 ctggtacttt gaggcttccg tattggtacg tgaggctttg atcagctcga tcgcccggtt
     1561 gtagtctttc gcggtcatcg ccttcgtcac gcgatcgttg aacagccgac cgatagtgtc
     1621 gtgattcgcc tgcagcttcg ctttttccgc aggaatcttg ttctcacgca gatctttgat
     1681 cgctttatcg acgcgattct tgccggaaaa atcaagagcg ccgagtttac tgcgttcgat
     1741 gatcagtcga tcgagttcga acgaatactg cagaatcttc aattcggcct gttgattctg
     1801 aagctcgaga ttgaccacat ctttgagatc ggcatcgccg ctggagttca ccccatcggg
     1861 gttgccggta atcgtgccgc cgtagtgccc tttgatggct ttgacaacaa agccgcggcc
     1921 ctcgatctga gcggtcaccg tcagggaacg cttgcgattg aagatgttcc cggtgacggt
     1981 ttcttttctg tcgagcttga acttcttgca gccatgctcg ttcaaaatct gttcgaacca
     2041 ggtaaagcgt tcatagccct tggtttcatc gacaaagaca atcgtcgcct ggacgatcgg
     2101 atacccgacc agataaccag catattccga tgtggcatag ccgttattct tcgcgacttc
     2161 accgatggtc ggggtcagag tcgtcaccgg agcgccagat cgagaggtct gattggcagt
     2221 ggtcttctgg gtctgaaccg gagcatcacc actgtacggg ttctgggcct ggaccacaga
     2281 acaacaactc actccgaatg agacgagggc ggcaagcgca agggaccggg acttaatcat
     2341 actcaatcct ccagaggtcc ggttaggtgg ctgggacaca taaagccaca gaatatctac
     2401 ggtcgaattc cgtcagaagt tttctgacaa tagttcattg tatccaattt tgcacaaaaa
     2461 agacaagtct gataacatga tcctgttcgt tcgaaattgt acggaggagg cacattgatg
     2521 acccgaaaac ttctgctggg ttctctcttc gtcctgctgc tcgccggcgc ggtgttcgcg
     2581 aacggttctt atgagctcgg tcaggccaag gcggccatgg accgcggcga cttccgctac
     2641 gccctcgacc agttccgctg gatcaccgga aacacctcat ttggtcctga ggttcgacgc
     2701 gaagcgatgt atttcatcgg tttcgtctat gtgaaaatgt ccgatccgtg gtcggccatt
     2761 gactccttcc gacgttttct cgataagtac ggcagctcat cgaacgagta tctgctgccc
     2821 gatgccatgt atgttctggg tcgcacctac gaagaagtcg gtcgtcacga cgacgctcgc
     2881 cgtctctatc gccgctgccg cgaacgcttt ccctataacg agttcgcccg caagtccgaa
     2941 gatcgcctgc gcattctcgg cgacagctac tatccgccca acccgccccc gtatccgccg
     3001 aatcctccgt acccacccag ctacggcatc actcccgaag tgcgcgacat gatcgaactg
     3061 gcgaagatgt ccccagactc ctacagcgct gatcagatgt tgctgaaggc ctgcaaccgc
     3121 gcccagctcg gagccgactt cgtcgctctc tccaaagcta cccgcaatca atacacccag
     3181 tatcagatct tcgatctgtg ccagaagtcc cgggtgtacc agatgatgac tccttacgag
     3241 gtcgtcgacc tcgccaagac taccaccaac tcgtactatc gcaaccagct gctgctggat
     3301 tacgccaaca aacgcgcccg ctacgccgaa gatttccgcg tgctgatgga tgcctgcacc
     3361 gatcacttca ccaaaacgca gatcatgcag atcgcccagc aaaagctggg gaacggtccc
     3421 atgttcaaca tctcgatgtc cgtagaagcc gctccggtga aaaccagcaa aaagaccgaa
     3481 acgcgcgtcg ccgatacgaa ggtgaaagct cagaagtccg cttctgatcc gttcgtcggc
     3541 tttcagagtg atgcggaaaa aatccgccgc gtcacgtggt tcctcgatgc cgttcagtac
     3601 aagaagggca ttgatgaaac ctcgaagctg ctgaaaaaag aggacatgac tctcgacgtg
     3661 gttcggcagg ccgtcaagga acgcgaatcc atgaagaagt tcgacaagct gcacggaaag
     3721 taaactcgtg tgatttcacg caccggcaag gctttccttg ccggtgcgtt tttctttcgg
     3781 agtctgaaca tgaaccagca cctgtttctg ttgcttgtga tttccctctg cgctgctccg
     3841 gtcttcgccg ccaacgatga cacggagatc atcaccatcg gcccgtcagt cgccactccg
     3901 cgccagccga cgccggccac gcctgccagg gagtcgccgc ccgcggaatt gtcgacgctg
     3961 cgtaccagag ctgaggccgg aaaccctgac gaccagctga ccctggggct cgccttgctc
     4021 gacaagcgct tcggagtcga tgacccggtc gaaggcgcga tctggatcac caaagccgcg
     4081 tcacagaacc acttcgaagc ccgtacggtt ctgggtcgtc tcaagcgtga cggccgcggt
     4141 gtaccacgca actacttcga agccgtgcgc cactttcgcg ccgcggctga cgccgggtat
     4201 cagaaagccc gcatcgaact tggcatgatg tatgccctgg ggcagggggt tcatcaggat
     4261 gcgctcgaag cctacaccct gctctgcctg gcctcaccca cgctgcaact cgcgccggcc
     4321 aacgaacttc acgcagagcg cgccatgctg gcaaagctcg agaaacaact ttccactgac
     4381 cagcgtgagc tggcactgga gcgcatgaac gatgcccgcg atcagtcttt gtccgagctc
     4441 atgaaacggc atccgggcaa gcagggcctt ccaccagccc ccatgaccga cccggtgaaa
     4501 aaatagacgg gcgtacaaga gtacctgaga gtactggttc agcgacgata cgactcaggc
     4561 tgaaccaata tctcgtcgat agatgacatt cgtgagatcg agccggcgaa tatcatcgta
     4621 gcaggtgttg cgggcttgtt cgagagtgtc tccaagagcg gtcagcgtca tcaggcgccc
     4681 accaccgaag cggtagctgg tactcccggg atcgatctcg ctgcccgagt gcgtgatcgt
     4741 gctttcccgg atgctttcaa acccggcaac gcgaggctgc gccgactcat cgagcggata
     4801 cccctgacgg gccagcacga ccgagaccgc aaagtgtcgc ccgaacgcca cctctgaacc
     4861 cagcgattcg agtcgccccc gggcacaatc gagtaacagg tcggccagat tgccgcgaaa
     4921 gcgggcgagt atgctttggg cttcggggtc gccgagacgc gcattgaatt cgagcacctt
     4981 gggcccgcgt gtcgtcagca tcagctgaaa cgacagaaag ccatgatacg tgagtttgtc
     5041 catacgtaac ccggcgaccg tcggctcgac gatgcggcgt tgaatctcgg cttcgacctc
     5101 gcgcgtcaga ccagggcagg ggctgaccgc acccatgcca ccagtggtcg ggccctcatc
     5161 atgctcgccg atcttcttgt aatcgcggca cgaagaaaaa ctgatccatt gctggccatc
     5221 ggtgagaatg gcgaacgagc actcgaggcc ctgcagacgt tcctgcagaa tcagtggcgg
     5281 cccatgggtt ttcagcaacc gatgcacagc gccgatcgct tcgtccgggt tgtcgaccac
     5341 ggcaacgccg gtacctgctg caaatccgtc gcatttgacg acctgcaccc acggattctc
     5401 gtgaatgaag gcttcggctt cggcccggtt cgccaccacg tgggtaaagg gggccggaat
     5461 gttgtgccgc gcaatgaatt tggcggcaaa tgccttgctg gtttcgatac gtccggcatc
     5521 ggccgccggc ccgatgacgg gaattccggc ccgggtcagc gcatcgaccg tccccagttc
     5581 gatgtatttg ggagaaccga ccaccgccag ttcgacacga tctttgacca cgttgatgag
     5641 atcagagttc gaggaaaacg gcagccgggt gcagaactct ttcatgccgg cgttgcctgg
     5701 gcaggcatag atcttatgaa ccgtcggcga tcgggaaagg ccgtaggcaa aggcgtgttc
     5761 gcgagccccc gctcccagaa tcaggatgtt cactgctcgt tctcccttcg tcatgcagcc
     5821 gtcgcagccc ggcaaggcgg cggtatgcta ctatgaaacc ggcttcgcgt caagctttgc
     5881 ccagcagtcg attgtggtat agtaaggttc gtgaggagat tctgccaacg aggtcacact
     5941 ccatgaaagt cacgcggctg ttttccgtct ccgtcggtct tttctttctg cttcttgcgg
     6001 gagtcacgtc cggcatcagt caaaccctgt attcgccggc cgtcacggac ctgttcaacc
     6061 gtccgggcag cagcagtccc cgcgacttgt acaagatggc tcacgagcac ttcaccgccg
     6121 gaaaactcga tgatgcccgt ctgctggcca tgcggattct tctggacggc catcgcagcc
     6181 agaatctgct cgatctgctc ggaacgatcg aagtcaaggc cgggcgccca ctcctggccg
     6241 gtgagtggct gcgcaaggcc atcagtctga acctggaaga caacgtcgcc cgcaaagtgc
     6301 tgctgcgtct gccgccacca ccgaggccga ttcccatgga tcagggaaag ctcgaagcgc
     6361 acttcgcgca gatttcgcag aaactcccat tgttgctcga gcgtctcaac tccccgagtc
     6421 tgcactttga cagcgtcctc gacgagatcg cccgcggcca gttctacaag gcactcgcgc
     6481 tcgccgaaga gtatgaaaag aagtccccgg gcgccaacgg caccggtctt acggcgctct
     6541 gcgcgctcta tcttggtcgc acccgtgacg cactgcaact ggtcgaacag gggctcaagc
     6601 tcgacccgta tcattccctg ctgctgttcg tgaaggtcct gggcaccgac actcaccccg
     6661 atacgtcagc accaaatcgg gcccgggcgc tgttcgacca ggaccagtgg gacgaagcac
     6721 tgaaagcctg cgatcaactg accgcgctgt ttcctcgttc tggcgacggg ctgctgatca
     6781 aggcccgcat tgcctacgaa cgccgacaat atgggcaggc ccgcagcctg gccgatcagg
     6841 cttcgattcg cgatcccgac catcccggca tcgatctgct gcgttccgac atcgctctgg
     6901 cccagaacca gcgcgaacag gcggcgcaac acatcgagcg cgctttccgg cgcgggtatc
     6961 atctgccgtc cgtcacgctg aaagccgcca tgttggccct gctcagcgaa caatccgacg
     7021 aagccgcgcg cgtgctcgat gaaaccgagg ctctgcagcc gttcgtcgac cgcgacgcgt
     7081 atccactctt cgtcgaact
//
LOCUS       DAORHD010000058         7066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10422_length_7066_cov_4.231351, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000058 DAORHD010000000
VERSION     DAORHD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7066
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10422_length_7066_cov_4.231351"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKO06_02235"
     CDS             complement(<1..373)
                     /locus_tag="PKO06_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68487.1"
                     /translation="MRISNNPLTLNRQESGNRVTRGFEFTLRAASDEDRRMEHSGMEA
                     TDPTMSFWNLVSSLPRQDQATMAANGLASRLAQNGINDSNLSLIRSFPARFTREEMLN
                     IEGLLKENPLLQMSGSASNRRL"
     gene            919..2211
                     /locus_tag="PKO06_02240"
     CDS             919..2211
                     /locus_tag="PKO06_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014186842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNV68488.1"
                     /translation="MQKHRKKGNMEESQSAGSLLRLAPTNEVKREEWSDWHWQMRNRI
                     TTVAQIARELKLNNQRLASLTCVEDRFRTVITPYYLSLANLNDPNDPILRQALPDPRE
                     LEMAGMGEEDPLDEEKDMPVPGLTHRYEDRALMVVTNACAMFCRHCTRKRIWHSDDAN
                     VSDENINAMIAYIRRTPSIRDVIVSGGDAFTLSTPRLESILKRLRAIPHVEVIRIGTR
                     TPVTIPMRIDNELCEMLDRYGPIWVNTQFNHPNEVTEESAKAIDRLIRHGVCVNNQSV
                     LLRGVNDDPETIRRLCRRLVQIKVRPYYLFQCDQIVGVEHFRTRISKGIEIMESLRGH
                     TTGFSIPTFVVDGPGGTGKIPVMPNYLISQSEQMAVLRNFEGAIIGYREAGEQVLRQV
                     HRTSTGVAGILAGHRQALVPMENRRMTRRKTRAAGAGL"
     gene            2189..3187
                     /locus_tag="PKO06_02245"
     CDS             2189..3187
                     /locus_tag="PKO06_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNV68489.1"
                     /translation="MRLALACNRSHLYQPVDGQPEDQFEEFDAPETVDAIAQTIASFG
                     HTVEILEADHTFPETLKQGMFDVVFNISEGFRGRCRESLVPAVCEMLGVPYTGSDPLT
                     MAISLDKNMAKKVLADKVPVPAGRVFTCAADLERSGAFDDLLFPALIKPNAEGSSKGI
                     RTFSRVENVSKAREVILWALKEYRGPALIEEFLPGVECTVGVVGNQNARVIGMMEIAP
                     KKVSQDQFVYSLETKRDYKNQVEYRVPPHLPESTKKEIEEVALGAFHGLDCRDFSRVD
                     VRLGADGRPRFIEVNPLPGLSPFKSDLVILARGMNIEYPELMRMILDSAFKRLGLA"
     gene            3200..4204
                     /locus_tag="PKO06_02250"
     CDS             3200..4204
                     /locus_tag="PKO06_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV68490.1"
                     /translation="MKVLILSNAPAPNQRWEAEERSDRGVLDAVSSISQALKANGHVV
                     ATGEIGERPEDLLQLLDREQPDTVFNLCETIRGNSRLEPAVPFLLRWRGVPFSGNGPQ
                     TISILLEKTTTKRLLRDHRLPTPAFHDLVSESDIDDWYIWPAILKPAAEDASLGIDAG
                     SVVRDREAARERFRLLAERFGTPVLIEQYIDGRELNVAVLETSEDLLLGVNEIDFSEM
                     PEHVPHIVTYNAKWVEESAEFLKSPVRAPANLPPEIDAEVRRLVRQTFAALHLAGYAR
                     IDLRLDTQNQPWILEVNPNPDISPGAGFVKALPEMGVSYEQAIEIIVQRACRTKGIPR
                     "
     gene            4201..4698
                     /locus_tag="PKO06_02255"
     CDS             4201..4698
                     /locus_tag="PKO06_02255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV68491.1"
                     /translation="MNVIIRDLRPTDRPAIATMLQQPEFFHPQDVDVALELVDDALKN
                     RKDSHYTFGVAELEGETVGYVCFGQRPMTVGTFDLYWICVNPHKKNNGVGRKLMRWAE
                     EQIFARNGHMVIVETAGRIGYKPTREFYLRVDYKEEARIKDFYGPNDDLVIYVKRNPA
                     LQKSS"
     gene            complement(4776..6485)
                     /locus_tag="PKO06_02260"
     CDS             complement(4776..6485)
                     /locus_tag="PKO06_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HNV68492.1"
                     /translation="MARMFPAKFPRHESEGSFSRAEKELFEGFRDQLGDEWVVYFGRK
                     FVAPKWKSRAPEDGEIDFLVGHPDLGILVIEVKGGTIRFDGENWYSQDMYGREHRIKD
                     PFEQASKNLHALVSLMERQPEWQGRHVADYAVAFPETETQPLNLPMHAPRGIILDQRD
                     VGRLPGRLSDILKHWSGQRSRGFANGPGILASLDRVLVPRPPIGLLISDAEKAIEELT
                     REQEKILDLLAWQKQAAIFGCAGSGKTYLACTKALQLKTLGLPRVLLTCHNKMISDEL
                     KKRLSGKGIEVMHFTSLCTQLCEWAGMPTRKIEPRPDEDPRDFKLRQSRYFDEELPEL
                     MLRVLEQHPEWKYDAVIIDEGQDFHDLWWLPLQEALKDPKESLFYVFYDDNQKLIEGK
                     RGNVPKELPRFLLRENVRNTRNIHQKLTHFYRGAQESISRAPAGPTVKLLSYESDDEM
                     ARLLDQTIVGLMRHADMRANRLVVLTPRNFQKSRVPRINLSNGYVLRQEKSAGEKEIY
                     YSNIFRFKGLESAVVIVVEIDEEFRRMSTFEELCYVAFSRPRSHLVLIGKREILNQLQ
                     KLA"
     gene            6668..>7066
                     /locus_tag="PKO06_02265"
     CDS             6668..>7066
                     /locus_tag="PKO06_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68493.1"
                     /translation="MHRRFSTTLLIITVTLAALTFGCGGGGGKNPATGYTGPTTTMNG
                     TLTVPALLNNALLTSIRGSQNTDSDIRAAFASATVLVNGSIPGTVVISPNSSNSMWPI
                     RLEGVPEAINDRYYLDVLARRFRLRAHVRTA"
BASE COUNT         1510 a         2122 c         1885 g         1549 t
ORIGIN      
        1 tcaatcgccg attgctggcg cttcccgaca tctgtaacag gggattctct ttcagcagac
       61 cctcaatgtt cagcatttcc tcacgagtaa accgggcagg gaagctgcgg atcaacgaca
      121 ggttggagtc attgatgcca ttctgagcca atcgtgaggc caagccattc gccgccatgg
      181 tcgcctgatc ctgccgcggt aacgacgaga cgagattcca gaacgacatg gtcggatcgg
      241 tcgcctccat gcccgagtgc tccatgcgac ggtcttcgtc cgaagcagct ctgagggtga
      301 attcaaaacc tcgggtcacg cgattgccac tctcctggcg gttcaaagtc agcggattgt
      361 tgctgattct catggtctgc ctccaagcga ttattccagt tcactaggac tatcggtcga
      421 ttgccctgcc gacgaaaggg gcgaattcag gattcgttgc gcaaatgtta tacatttttt
      481 tctccccccc ttgtgaggta cgtacgccga tgttataagg agacaaaata cgttcttgat
      541 cagcatttga taattgacag gcggcagaca catatgacag gtgaagatct cgtctcactt
      601 attcgcaggc cccgccgact caggcgtggg tatgtcacac tcgcgatcca tttttcgctt
      661 caatgccccc cattttcggg aggctttttt ctttgcccag agggtcgagg tcaagcggta
      721 acccaaggcg gcaggtaaca tcgcccgggt ttcgcccagc gttttccgca acagatagca
      781 ccaggataac gctttcgaaa ctcgcggttc gctgtttccg gtttcaagga acgggagcct
      841 tgaaacaacg cactgaaagc acattgggct cacgcccacc ctggacccgg ggccaccggc
      901 aaggaaaccc cggaacattt gcagaagcat cgaaagaagg gaaacatgga ggaatcacag
      961 tccgccggga gcctgctgcg gctcgctccc accaacgaag tgaagaggga agaatggtcc
     1021 gactggcact ggcagatgcg caaccgcatc acgaccgtgg cccagattgc gagagaactg
     1081 aagctcaata accaacgcct cgcgtctctc acctgtgtcg aagatcgttt tcgcaccgtc
     1141 atcaccccgt attacctgtc tctggccaat ctgaacgacc cgaacgatcc gattctgcgt
     1201 caggctctgc ccgacccgcg cgaactggaa atggcgggca tgggcgaaga agatccgctc
     1261 gacgaagaaa aggatatgcc ggttcccggt ctgactcacc ggtatgaaga tcgagcgctg
     1321 atggtcgtga caaacgcctg cgccatgttc tgccgacact gcacgcgcaa gcgcatctgg
     1381 cattccgatg acgccaacgt ctcggatgaa aacatcaacg ccatgatcgc ctacattcgg
     1441 cggactccga gtatccgcga tgtcatcgtc tctggcggcg acgcgttcac cctgtcgacg
     1501 ccgcgcctcg aatcgattct caaacgactg cgggccattc cacatgtcga agtgattcgt
     1561 atcggcacgc gcactcctgt gaccattccg atgcgcattg acaacgaact ctgcgagatg
     1621 ctcgaccggt acggtccgat ctgggtcaac acccagttca atcacccgaa cgaagttacc
     1681 gaagagtcgg cgaaagccat tgaccgcctg atccgccacg gcgtctgcgt caacaatcag
     1741 tccgtgttgc tgcgcggcgt caatgatgat cccgagacga ttcgacgttt gtgccgacgc
     1801 ctggttcaaa tcaaggtgcg cccgtactat ctgttccaat gcgaccagat cgtcggggtc
     1861 gaacacttcc gcacccgcat cagcaaaggc atcgaaatca tggagtcact gcgcgggcac
     1921 acaacgggat tctcgattcc caccttcgtc gtcgacgggc ctggcggcac cgggaagatt
     1981 ccggtcatgc ccaactacct gatcagccag tccgagcaga tggccgtttt gcgcaacttc
     2041 gagggcgcca tcatcgggta ccgcgaagcc ggtgaacagg tcctgcgaca ggttcatcgc
     2101 acctcgaccg gggtggcagg cattctcgcc gggcatcgcc aggcgcttgt tccgatggaa
     2161 aaccgtcgga tgacccggag gaagacccgt gcggctggcg ctggcctgta atcgttcgca
     2221 tctctatcag ccggtcgacg gacaacccga agaccagttc gaagaattcg acgcccctga
     2281 gaccgtagac gcgatcgcgc aaacgatcgc gtctttcggt cacacggtcg agatcctcga
     2341 agccgatcac acttttcccg aaaccctgaa acagggcatg ttcgacgtgg tgttcaacat
     2401 ctctgagggg ttcaggggtc gttgtcgcga atcgctggtt ccggctgtct gcgaaatgct
     2461 cggtgtgccg tataccggtt ctgatccgct caccatggct atctcgctcg ataagaacat
     2521 ggccaagaaa gtgctggcgg acaaggttcc cgtacctgcc ggccgggtct tcacctgcgc
     2581 cgccgacctc gaacgttcgg gtgcgttcga cgacctcctg ttcccggcgt tgatcaagcc
     2641 gaacgcggaa gggtcgagca aagggattcg caccttctcg cgggtcgaga atgtctcaaa
     2701 ggctcgtgaa gtcattctct gggctctcaa ggagtatcgc ggtccggcgc tgatcgaaga
     2761 gtttctgccc ggagtcgagt gcaccgtggg tgtcgtcggc aaccagaatg cgcgagtgat
     2821 cgggatgatg gagatcgcgc ctaaaaaagt ctcgcaggat cagttcgtct actcactcga
     2881 gaccaagcgc gactacaaga accaggttga ataccgggtg ccgccgcatc tgcctgaaag
     2941 taccaaaaaa gagatcgagg aagtcgctct cggagcattt cacggccttg actgtcgtga
     3001 tttttcgcgc gttgatgttc gcctcggtgc cgacgggcga ccccggttca tcgaggtcaa
     3061 tccgttaccc gggttgtccc ccttcaagag cgatctggtc attctggcgc gcggtatgaa
     3121 cattgagtac cccgaactga tgcgaatgat tctggattcc gccttcaagc gactcggact
     3181 cgcctagccg ttctcacgca tgaaggtctt gattctttcg aatgctccag cgccgaacca
     3241 acgctgggaa gccgaagagc gctccgatcg cggagttctt gacgcggttt cctcgatttc
     3301 tcaggcactg aaagcgaacg gacatgtcgt cgccaccggc gagatcggcg agcgtccgga
     3361 agaccttctg cagctgctcg accgcgagca acctgacacc gtcttcaatc tgtgcgaaac
     3421 catacgcggc aacagtcgtc tcgaaccggc ggttcccttt cttctgcgat ggcgcggagt
     3481 tcccttcagc ggcaacggtc cgcagactat ctcgattctg ctcgagaaga cgacgaccaa
     3541 gcgacttctt cgcgatcacc gtcttccaac acctgctttt catgatcttg tgagcgaatc
     3601 agatattgac gactggtaca tatggccggc aattctcaag ccggcggctg aagacgcttc
     3661 actcggaatc gatgccggca gcgtggttcg tgaccgggag gcagcccgcg aacgctttcg
     3721 cttactggcc gagcgattcg gcacccccgt gttgatcgag cagtatatcg acggacgcga
     3781 actcaacgtc gcggttctcg agacttctga ggatctgctt ctgggtgtga atgagatcga
     3841 tttttccgag atgcctgagc atgttcccca catcgtcacc tacaacgcca agtgggtcga
     3901 agaatcggcc gagtttctca agtccccggt gcgggccccc gccaatctgc cacccgagat
     3961 cgatgccgag gtgcgccgtc tggttcggca gacgtttgcc gccctgcatc tggccggata
     4021 tgccagaatc gatcttcgtc tcgataccca gaaccagccc tggatcctcg aagtgaatcc
     4081 caacccggac atttcccccg gcgcgggatt cgtgaaagcc ctgcccgaga tgggcgtgtc
     4141 ctatgagcaa gccatcgaaa tcatcgtgca gagagcctgc cgaaccaaag gaattccccg
     4201 atgaacgtca tcatccgcga cctgcgaccc accgaccggc ccgcgattgc taccatgctg
     4261 cagcaaccag aattctttca cccccaggat gtcgacgtcg ctctcgaact ggtggatgac
     4321 gctctcaaga accggaagga ttcgcattac acctttggtg tcgccgaact ggaaggtgaa
     4381 accgtcggct atgtctgttt cggccagcgc cccatgactg tcggcacctt cgacctctac
     4441 tggatctgcg tgaatcccca taagaaaaac aatggggtgg ggcgaaaact gatgcgctgg
     4501 gccgaagagc agatcttcgc ccgcaacggc cacatggtca tcgtcgagac ggctgggaga
     4561 atcggctaca aacccacccg cgaattttac ctgcgcgtcg attacaagga agaggcccgc
     4621 atcaaggatt tctacggccc gaatgacgat ctggtcatct acgtcaaacg gaatccggca
     4681 ctgcagaaat catcgtaacg gtcgatccac cttcagagcc tgcgcgcgaa tcattgactc
     4741 cgtagagagg tttcagaacc cgtcagccgg tactctcaag ccagcttctg cagttgattc
     4801 agaatctctc gtttgccaat gagtaccaga tgactgcgcg gccgcgagaa cgcgacatag
     4861 cacagttcct caaaggtgct catgcgacga aactcctcat cgatctcgac cacgatcaca
     4921 acagcgcttt caagcccctt gaaccggaaa atgttcgagt aatagatctc tttctccccc
     4981 gctgactttt cctggcgcag cacatacccg ttgctcagat tgatacgggg cacacggctc
     5041 ttctgaaagt tacgcggggt caataccacc agacgattgg ctcgcatgtc cgcgtggcgc
     5101 atcaaaccaa caatcgtctg atcaagcagt cgagccattt cgtcgtctga ctcatacgaa
     5161 agcagtttca ccgtcgggcc ggcgggtgca cgactgatcg attcctgagc cccgcgataa
     5221 aaatgcgtca gcttctggtg aatgttgcgg gtgttgcgca cgttctcccg cagcaggaat
     5281 cgaggcaact ccttcgggac attgccgcgc tttccttcaa tgagcttctg gttgtcatcg
     5341 tagaaaacgt agaacaggct ctccttcgga tccttgagtg cttcctgcag gggcagccac
     5401 cacagatcat gaaagtcctg tccctcatcg atgatcacgg catcgtattt ccattccgga
     5461 tgctgttcga gcacccgcag catcaactcc ggcagttctt catcgaagta gcggctttgt
     5521 cgcagcttga agtctcgcgg atcttcgtcc ggtcgcggct cgatcttccg ggttggcatt
     5581 cctgcccact cgcacagctg cgtgcacaga ctggtgaaat gcatcacttc aatccctttg
     5641 cccgaaagcc gctttttcag ctcatcgctg atcattttgt tgtgacaggt cagcaacacc
     5701 cgcggcaaac ccagcgtttt cagctgcagc gcttttgtac aagcaagata cgtctttcca
     5761 gagccagcac agccaaatat ggctgcctgc ttttgccatg ccaacagatc cagaatcttc
     5821 tcctgctcgc gtgtcagctc ttcgatcgct ttctcagcat cgctgatcag caggccgatc
     5881 ggtggtcgag gcaccagaac ccggtcaaga cttgccagaa tacccgggcc attggcgaat
     5941 ccacgtgacc gctgtcccga ccagtgtttg agaatgtcgg acagacggcc gggcagacga
     6001 cccacatcgc gctgatcgag aatgatgccg cgcggtgcat gcatcggcag attcaagggt
     6061 tgtgtctctg tctcgggaaa ggcgaccgca tagtcggcga catgtcgccc ctgccattca
     6121 ggttgtcgtt ccatgagaga caccagcgca tgcaggttct tggaagcctg ttcaaagggg
     6181 tccttgatgc gatgctcgcg tccatacatg tcttgagagt accagttttc tccatcgaat
     6241 cttatggtcc cacccttgac ttcgatgacg agtatgccaa gatcggggtg gccgaccaga
     6301 aagtcgattt ccccgtcctc cggagctcga cttttccatt tgggagcgac gaacttgcgc
     6361 ccaaaataca cgacccactc gtctccgagc tggtctctga atccctcgaa cagctccttt
     6421 tccgctcgac tgaacgatcc ttcgctctcg tggcgaggga actttgcggg aaacatccgc
     6481 gccatgacac acctctcgtc tacgttcttc tttctggata ccttactttt caatcaatct
     6541 gcaacccctg cggcaggtgt cggtcgatat cgacaagaat ttgcatctcg ggccattgac
     6601 ctggtacccg aaccggtact atgatcctgg atgtctgtac ccactctgac acttttgagg
     6661 tggtctcatg catcggcgtt tttccacgac cctgttgatc attaccgtca ctctggcggc
     6721 gctcacgttc ggttgcgggg gtggcggggg taaaaatcca gccaccggtt acaccggtcc
     6781 gaccaccacg atgaacggca cactgacggt tccggctctt ctcaacaatg ccctgctcac
     6841 ctcgatcaga ggctcacaga ataccgatag tgatattcga gcggctttcg ccagtgccac
     6901 cgttctggtc aacggctcga tccccggcac cgtggtgatt tcgcccaata gttcaaattc
     6961 gatgtggcca attcggctcg aaggcgtgcc tgaagccatc aacgaccggt actatctcga
     7021 tgtgttggct cgacgttttc ggttgcgtgc ccatgttcgt acggcc
//
LOCUS       DAORHD010000059         7036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10491_length_7036_cov_3.741011, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000059 DAORHD010000000
VERSION     DAORHD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10491_length_7036_cov_3.741011"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..3935
                     /locus_tag="PKO06_02270"
     CDS             201..3935
                     /locus_tag="PKO06_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68494.1"
                     /translation="MNRSLGFLIAGFLLIFASAAFGTMDYAHPEAISLGLQQLESLYT
                     AEKYDEAISYARNLLGKIPKDQPQKQRAMDLLTLSLDRQAQKRLARETTQKREQNRKS
                     SEQLITEAAALMGEKKYAEALPKFKQAAQLFGGDAETFFMLGYAHHKLGQEADAYLAY
                     KRCAQLNGDHPRALFHLSQLSYKLKRFQESESYSRKLIQTLNTRFDESRQILLEQRAA
                     SLNDKALATARSMVSMKKNLAQGYYLLGLLTARRGNWQEATDSFVTAIRIDPRLTEIY
                     FHLGMAKFKIGQFHQASLAFERALFIGETTIREETARSKKLLDEGKADDAVAIGVKVK
                     ELENRMALVNYGIALCQLKKMETGAALESIEKTLSFKPDFLQARFARAVILASQREYQ
                     QALYEMRQILKVAPPNSPEAKKAIRSIKLLMDQSLASTQGIKPAPKKQGVPTTDVNRF
                     VKDMHGLGDRKQEEDWSDLYPTINEIVDLAARRNFATALRKALHLQKVHPNLTQAWSI
                     AGKCYMEMGRFEDALDSFKKALARNPNDPDSLGNIAYLNALKGVELDKSLAMAKQAIE
                     FQPNNAAFHHTLGWVWFRKGEMEKAIGELQTALQLRPEYPLARYNLALAYYLMGNFDQ
                     ALAGFEAVMSSQPDNMKARLFRGITLAKMQKVPEAVAALDELKKKLPEKEVLVKVVTE
                     LHDRLKLAHERKTDIPVPEIKHTAPIAQLLEKARKYRQLGLVNRAKELYLECQRLHPE
                     AFQPWYELGAMYAESGLSAPAKRAWEQALKLKPNDYTLQVNIGRMQFKLNDRAHARTA
                     FTAAQALKPEDPEPPDYLGLIAYEEARFESAESHALAALRLKPRHFKAMALLGMSRIR
                     LGRYEAARDAYETLYARAPRDSSIRRHARKKLWIIARLMQPNRAPSFPDAELHKEELE
                     KRAAGKTSGDSQDLNAIEVKKAAADKWNRLSTEDKIWLYRRLEPFGGAGKVPADIAAQ
                     AAVSSIAAPMTTEEKLLILKKLDSFKTLAKAYPPPPPRMSSKYKLGKPVKPKRSADDA
                     DPFIKQALESAEKGFITEAVSALEKGRAISPRNLDLLINLGFLELQLGNFKSSFEVFA
                     QAALHHPKSPLARLCLGHMYWLGGRGPEAVDQWKLIGTLDMETDAEFSILQRAEKVWK
                     RVLDNNPTDSDAHSNLGIVYLFSGKATQAIAEFESVLQLAPQRIEHRFYAAQAYSLLY
                     ISGKNKNHKREARSRLQELANTGTPFPHSPTLYAFLEKLS"
     gene            3932..4918
                     /locus_tag="PKO06_02275"
     CDS             3932..4918
                     /locus_tag="PKO06_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68495.1"
                     /translation="MRIPLLPDQSSCVGVPRRQRFVAAVILVLLISWVTVAQAALPRP
                     LDLPELGDYEIYSIGGAGSATGKRILLPESPRQALNEDGLIGYFSGATADKLPPKQLW
                     AALSYQYSQLTSRRGRAFRAGEDGHLGSVYLRANYIGDWAEWAISIPFHEYSLAAPDT
                     YGRPSDENVGVGNVKLGWKATYLPDRSYYRFSYGAVAHLTAGDPDIHLPITARSEDEV
                     KLFGCVTTKETDRATANLELGTILNSRGDENRFTYRAGFSYQPTDPSVFICELAGEVL
                     TGNDKDTMDLISGLRLAPARTVVFEMTYTRNLRTYREYGFDHRFGAGATIRW"
     gene            4946..6589
                     /locus_tag="PKO06_02280"
     CDS             4946..6589
                     /locus_tag="PKO06_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HNV68496.1"
                     /translation="MESSLSHWFTRTLAAAYHHPPVETPTAALEQCGIDPSPFPWLSN
                     ISPASYPLRGDRLEQWPDLEQRQRSAKRTHGQYFTPEVLVTHLLQTLAPLPTSGRILD
                     PACGAGGFLVPLAGHLADVQPDTFLDRLYGYDIDPDALLICLGRLLSRFPHRGVPTLE
                     QRDFLLSPPEDQFRLIIGNPPYRVNLPEATRTALAQRYTTTEGEKDLYTFFLEAGIGA
                     LTHDGDLVMLTSHTYLVNHQCQRIRDHLFRNHQARSLYFLPFRFFPMAPGVIPVITHV
                     SSSRATEQALQIYDQYSLTNGWNSRHAVKPELLRSSTGLAQIRLSPDARQLFDQMQQE
                     HQPLGNLARVGVGIQESVTRGRQISRFVRATKDSDADVPVLRGREIDQFKVQWGGNYL
                     TYGPHLTYPGPEEIFREPKILYQNLRNESLPLRLVAALDTDGFFPKNSLSFIARPTPP
                     FTLEFLVGLLNSPLVNAWFAGRYYSFHITVTQVRSIPIPTATEPLRHEIEHLVRQLKN
                     TELETREGERLQSHLSELVIRCYFPGEPIARIRHVLGLG"
     gene            6740..>7036
                     /locus_tag="PKO06_02285"
     CDS             6740..>7036
                     /locus_tag="PKO06_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV68497.1"
                     /translation="MPTYGYECTKCAHKFEVFHSMNSVEPRHCPRCEGLGKKLLSASS
                     IIFKGSGFYTTDYRDSGYVEAQKRDSTTPSSTTAGSNAKPAETTVAPAASEKPTA"
BASE COUNT         1593 a         2148 c         1891 g         1404 t
ORIGIN      
        1 gctcagagaa aacaatcgcc gcaaagagaa tcaggttttt cgtccactgt ttggggcgaa
       61 gtgtttcgag ccacgccggt actctcatga tgctcagagt ctactgaacc cgctcgggga
      121 aggcaagaat ttgccacgga cgaaccgaaa aggatgagat ttcatactcg cgcccaatct
      181 gccgatacaa gaagataagc atgaacaggt ctcttggctt tctcattgcc ggatttcttc
      241 tgatcttcgc ttctgcggcg ttcggaacga tggactacgc ccacccggaa gcgatcagcc
      301 ttggtttaca gcaactggag agtttgtata ccgctgagaa gtatgatgag gccatttcct
      361 acgcccgcaa ccttctggga aaaattccca aggaccagcc gcaaaaacag cgggcgatgg
      421 atcttctcac actgtcgctc gaccggcaag cgcagaaacg actcgctcgt gagaccactc
      481 agaaacgcga gcaaaatcgg aagagttctg aacaactgat cacggaagct gcggcgctca
      541 tgggcgagaa aaagtatgcc gaggcgctgc caaagttcaa gcaggccgcc cagctcttcg
      601 gcggtgacgc cgaaaccttc ttcatgctgg gctacgcgca ccacaaactc ggtcaggaag
      661 ccgatgcgta tcttgcctac aagcgctgcg cccagttgaa tggcgatcat ccacgggcgc
      721 tgttccacct gtcgcagctg agctacaaac tcaagcgctt tcaggaatcg gaatcgtatt
      781 cgagaaagct gattcagacg ctcaacacca ggttcgatga gtcccggcag attctgctcg
      841 agcagcgcgc cgccagcctg aacgacaaag ctctggcaac cgcgcgcagc atggtctcga
      901 tgaagaagaa tctcgcccag ggctactatc tgctgggact gctcacggct cgtcgcggca
      961 actggcagga ggctacggac tcgttcgtta ccgcgatccg catcgatccg cgcctcaccg
     1021 agatctactt tcacctcgga atggcgaagt tcaagattgg gcagtttcat caagcgtcac
     1081 tggcttttga acgagcgctg ttcatcggcg aaaccaccat tcgcgaagag accgcgcgca
     1141 gcaagaagct tctcgacgaa ggcaaggcgg atgatgcggt cgccatcggg gtcaaggtga
     1201 aggaactcga aaaccgcatg gcactggtga actacggcat cgctctctgc caactgaaga
     1261 aaatggagac aggcgcggct ctcgagtcga tcgagaaaac tctgtctttc aagcccgact
     1321 ttctgcaggc tcgattcgcc cgcgccgtca ttctggcatc acaacgggag tatcaacaag
     1381 cactgtatga gatgcgccag atcctgaagg tggctccgcc caacagtccc gaagccaaga
     1441 aggccattcg ctcaatcaag ctgttgatgg atcagtcact cgccagcact caggggatca
     1501 aacctgcccc caagaagcag ggagttccga ccaccgatgt caaccgtttc gttaaagaca
     1561 tgcacggtct tggcgaccgc aagcaggaag aagactggtc cgacttgtac ccgaccatca
     1621 acgagatcgt cgatctcgcc gctcgccgca actttgcgac cgcactgcgc aaggctcttc
     1681 acctgcagaa ggtccatccg aacctgaccc aggcctggtc gatcgccggc aagtgctaca
     1741 tggagatggg acgtttcgag gacgctcttg attccttcaa gaaagccctg gctcgcaatc
     1801 caaatgatcc tgacagcctc ggcaacatcg cctatctcaa cgcgttgaag ggcgtcgagc
     1861 tcgacaagtc cctggccatg gcaaaacagg ccatcgagtt tcagccaaac aacgcggcat
     1921 ttcatcacac tctgggctgg gtctggttca gaaaagggga gatggaaaag gcgatcggcg
     1981 agcttcagac tgccctgcaa ttgcggcccg agtatccgct cgcccggtac aatctggcac
     2041 tcgcctacta tctgatgggc aattttgatc aagctctcgc ggggttcgaa gcagtcatgt
     2101 cgtcacagcc cgacaacatg aaagctcggt tgtttcgcgg catcacgctg gccaagatgc
     2161 agaaagtccc agaagccgtt gccgctcttg atgagctgaa aaagaaactg ccagagaaag
     2221 aagtgctcgt gaaggtggtc accgaactgc acgaccggct gaaactcgca cacgagagaa
     2281 aaaccgacat tccggttcct gaaatcaagc atacggctcc catcgcccaa ctgctggaaa
     2341 aggcccgaaa gtatcgccaa ctgggtctgg tcaaccgggc aaaggaactg tatctcgagt
     2401 gtcagcgcct gcatccggaa gcttttcagc cctggtatga gctgggcgcc atgtatgcgg
     2461 aatcggggtt gagtgctccc gccaagcgcg cctgggaaca ggcgttgaag ctgaaaccca
     2521 acgattacac cctgcaggtg aacatcggcc gcatgcagtt caaactcaat gatcgcgccc
     2581 atgcgcgcac ggcctttacg gccgctcagg cgttgaaacc cgaagacccc gaaccaccag
     2641 actatctcgg gctcatcgcg tatgaagagg cgcgcttcga atcggcggaa agtcatgcgc
     2701 tcgcggctct gcgcctgaaa ccgcgccatt tcaaagccat ggccctgctc ggcatgtccc
     2761 gcatccgcct cggtcgttac gaggcggcac gtgatgcgta tgaaacgctg tatgcgcgcg
     2821 cgccacgcga ctcctcgatt cgacggcacg cccgcaagaa gctctggatc atcgctcgat
     2881 tgatgcagcc gaatcgcgct ccgtcgttcc ccgatgccga gctgcacaag gaagaactgg
     2941 agaagcgggc tgcgggcaag acctcgggtg acagccagga tctgaacgcc atcgaagtga
     3001 agaaagccgc agccgacaag tggaaccgac tctcgaccga agacaagatc tggttgtatc
     3061 gtcgactcga accctttggc ggcgcgggaa aagtcccagc ggacatcgcg gcacaggccg
     3121 ctgtctcctc gattgccgca ccgatgacta ccgaagaaaa gctgctgatt ctcaagaagc
     3181 tcgactcatt taaaactctg gccaaagcct acccgccacc gccaccgcgc atgagttcga
     3241 agtacaagtt gggaaaaccg gtcaagccca agcgaagtgc ggatgacgct gatccattca
     3301 taaaacaagc cctcgagtcc gccgagaaag gattcatcac cgaggcggtt tccgcgctgg
     3361 aaaagggtcg cgcgatctcc ccgcgcaacc tggatctgct catcaacctc ggattcctcg
     3421 aactgcagct gggcaacttc aagagttcgt tcgaagtctt cgcccaggcc gcgctgcatc
     3481 accccaaatc gccgctggcc cgcctgtgtc tcggacacat gtattggctg ggtggtcgtg
     3541 ggccagaagc cgttgatcaa tggaaactga tcgggactct cgacatggaa accgacgccg
     3601 agttcagcat tctacagaga gccgaaaaag tctggaagcg agtgctggac aataatccga
     3661 cggattcgga cgctcactcg aacctgggta tcgtctatct gttttcggga aaggcaactc
     3721 aggctattgc cgagttcgaa agcgttctgc aactcgcccc ccagcgaatc gaacaccggt
     3781 tttatgcagc ccaggcttac tcgctgttgt acatcagcgg caagaacaag aaccacaagc
     3841 gcgaagcccg atcccgcctg caggaactgg ccaacaccgg aacgccgttc ccgcactcgc
     3901 cgaccctgta tgccttcctc gagaaactct catgagaata cccttgttgc ccgatcaatc
     3961 gtcatgcgtc ggtgttccac gccgtcagcg cttcgtggcg gcagtcatcc tggttcttct
     4021 gatcagctgg gttacagttg cacaggccgc tctgccgcgc ccgcttgatc tgccggaact
     4081 tggagactac gagatctact cgatcggcgg tgccggcagc gccaccggca aacgtattct
     4141 gctccccgaa tcgccgcgtc aggcgctgaa tgaagatggt ttgatcgggt acttttcggg
     4201 agcgacggcg gacaaacttc ccccgaagca gctgtgggcg gcactatcgt atcagtattc
     4261 gcagctcacc tcccgtcgcg ggcgagcgtt ccgggctggt gaggacggac acctcgggtc
     4321 ggtctatctc cgcgccaact atatcggcga ctgggctgaa tgggccatca gcattccgtt
     4381 tcatgaatac agtctggcgg ctcccgacac gtatggccgg ccgtccgatg agaacgtcgg
     4441 cgtcggcaat gtgaaactgg gctggaaagc gacgtatctt ccggaccgca gttactaccg
     4501 gttcagttat ggtgctgtcg cgcacctcac ggccggcgac ccggacattc atctgccgat
     4561 cactgcccgc agcgaagatg aggtcaagct tttcggctgt gtcaccacga aggaaaccga
     4621 ccgagccacg gcaaacctcg agttgggcac gattctgaac tcgcggggcg acgagaaccg
     4681 gttcacctat cgtgccgggt tcagctatca gccgaccgat ccgtcggtct tcatctgcga
     4741 actcgcaggc gaagttctga ccggcaatga caaagacacg atggatctga tctcgggttt
     4801 gcgcctggcc cccgcccgaa cggtcgtctt cgaaatgacc tacacgcgca atctgcgtac
     4861 ctatcgcgaa tacgggttcg atcatcgatt cggcgccggc gcgacgattc gctggtaacc
     4921 accttttcga cagcctcagc tgacgatgga atccagctta tcgcactggt tcacccggac
     4981 actcgccgca gcctatcatc atccgcccgt tgaaacgccg accgccgctc ttgagcagtg
     5041 cggcattgat ccatcgccat tcccctggct gtcgaacatc tcgccggcat cctaccccct
     5101 tcgcggagac aggctggagc aatggcccga cctggaacaa cggcagcgca gtgccaagcg
     5161 cacacacggg caatacttca cgcctgaagt gctggtcacc catctgctgc aaacccttgc
     5221 gcctttgccg acatcaggca ggattctgga tcccgcgtgc ggcgcgggtg gctttctggt
     5281 tcctctcgcc ggccatctcg ccgacgttca acccgacacg tttctcgacc gtctgtacgg
     5341 gtatgacatt gatcctgatg cattgctgat ctgcctcggg cgcctgctga gccgttttcc
     5401 tcatcgtggt gttccgaccc tcgagcaacg ggactttctg ctttccccgc ccgaagacca
     5461 gtttcggttg atcatcggga acccgcccta tcgcgtcaat ctgcccgaag cgactcgcac
     5521 tgccctggcg cagcgctata ccaccacaga aggagaaaag gatctctaca ccttctttct
     5581 cgaagcaggt atcggagcgc tcacgcacga cggcgatctg gtcatgctga cctcgcatac
     5641 ctatctggtc aaccaccaat gccagcgcat cagagaccat ctcttcagga accatcaggc
     5701 gcgcagcctg tactttctcc ctttccgctt tttccccatg gcgccagggg tgattccggt
     5761 catcacgcat gtgagttcat cacgcgctac cgaacaggct ctgcagatct acgaccagta
     5821 ctctctgacg aacgggtgga actcgcgaca tgcggtcaaa cctgaactac tgcgctcttc
     5881 gacgggcctg gcacagatac ggttgtcgcc cgacgcgcga cagctgtttg atcagatgca
     5941 gcaagagcat caacctctgg gaaacctcgc ccgtgtcggc gtaggcattc aggaatcagt
     6001 gacacggggt cggcagatct cgcggtttgt tcgcgccacg aaagacagcg atgcagatgt
     6061 accggttctt cggggtcgag aaatcgatca attcaaagtt cagtggggtg gaaactatct
     6121 gacttatggc ccgcatctca catatccggg tccggaagag atcttccgcg aacccaagat
     6181 tctgtatcag aatctgcgca acgagtcgct gcccctcaga ctggtcgccg ctcttgatac
     6241 cgacgggttc tttcccaaaa actctctttc gtttattgcc cgccccactc cgccgttcac
     6301 gctcgagttt ctcgtcgggc ttctgaactc tccgctcgtg aacgcctggt tcgccggcag
     6361 atactacagt ttccacatca ccgtcactca ggtgcggtcg attccgattc cgaccgccac
     6421 agaaccgtta cgtcacgaga tcgagcacct cgtgcgacag ctgaaaaaca cggaactgga
     6481 aacccgcgaa ggtgagagac tccagtctca cctttccgaa ctcgtgatcc gatgctattt
     6541 tcccggggaa cccatcgcgc ggatcaggca cgtgctcgga ctcggttgat ttcgtcgcca
     6601 cggcctgctg ccgggccaga gagaaacgat tgtctgtcac gaaatgttgt ggtatattag
     6661 cactcatctc attcgagtgc taagtcgttc gaatggctgt catacacgat cacataccac
     6721 cttggcctgg aggtacatca tgccaaccta cggatatgaa tgcaccaagt gcgcacataa
     6781 gttcgaagtg tttcactcga tgaattcggt cgaacccaga cactgtcccc gatgcgaagg
     6841 tctgggcaaa aagctgttgt cagcctcctc gattattttc aagggttccg ggttctatac
     6901 caccgactat cgtgactcag gatatgtcga agcccagaaa cgtgattcca caaccccgtc
     6961 ttcgacgacg gccggttcca acgcgaagcc ggcggaaaca accgtcgcac cagcggcaag
     7021 cgagaagccc accgcg
//
LOCUS       DAORHD010000060         7028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10510_length_7028_cov_3.795784, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000060 DAORHD010000000
VERSION     DAORHD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10510_length_7028_cov_3.795784"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..4030
                     /locus_tag="PKO06_02290"
     CDS             56..4030
                     /locus_tag="PKO06_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68498.1"
                     /translation="MSSSPRPASGKTERIDDEVLKQIQIRNLSVQIPRPRDFLPDQTL
                     VRLLRLPPGNIDVPGVDVTIEQHLPAVTGQHSEPTIDHTIRVVPRQASGTVRPLIFGL
                     FSRARQNDDWNCAVEVLGQRLALGVQRQGEHSRRYTIVDERNRDRFYLRFSLNQDNTV
                     SHSRMTGLNGSIDVPELRRVAEWLRLNLDVGGALRLTLKSEDTAELIDTQMHLEAQSL
                     RLMLLPALSLENGIISCQLQKREGTVTNLDGTWFGRRVEQRGVFQLASHGGATASFSA
                     RVAEEPLRWTLQLEPQRDRARRIAIEVARSAGELRIAATQNASGSNARWRDGDLELRV
                     RDGQRITDLIPSPLIPSRFAATWASMTTTVDLLGPLHVTGQILSDQQFDQARVELRLD
                     GASMRDPRQNDSRLVLQGRILVASRSLELREVGLRLDRLSLIASGLVDLGLNGYVWQR
                     GRVDVRGELTGTGDWDLDARLLQRLFALSSPPGFERLRVRGQRLLSLSVGPDVGVPTL
                     TLLAEKALLTRGKAIWNCEPLNVRVFSPTPFRFEKPLPPLLQTELAGGIFGARCLASA
                     AIDLDRREWLQIGMSADGPDLAAIQRTLADVPEVGSYLRKHQLTLGGSFQLRVSGKGP
                     LAWPVMQGEWRFPALAIAVPGLQAQVPCQIAIETPAPGTYRGLVKTSNTKIERRGVPF
                     VLDSLKATWQYERVPTPRHHLLTLALDGKLFDTELRGTIQYLPRLRRVQQARLQFEGR
                     NLEKLSREVGRVGQFAFPFTMRGGIRGEWVASGTLPDLTSRATCTWNGIEVQVPIRQS
                     DKPLVSLDVRDLSGTLDLVQNARDRFDVELSSGTARWLNAPLRLSGLGHLARQESGRW
                     IPVTDRLELDLADLPIRDAVAFWKHTAGARDALPDVTDLSGTVSTRVRLSGLRNRYLG
                     EGSLILKAAAFRLPGVALPLSELNGTIALGRRTASEAVSLSVDGLRGRFGRADVRIPR
                     GRVIDPLGSCRIAFDGVVSNILPGDLLSLCGGWRLPALSFPREGSYAGTLNLSGTAAS
                     PKARVSVTGDEKTEILLRTPQREYRLPVGPCQISFAYEPTNGKMSVDPGQIGVLDGLI
                     RIDAASAELRSGAARQFEVRGTFSDIDLGRFSRDGESLVRGTLVGTFRANQTPDRHRE
                     LLVHLGMEKLKILTLPVDQTLMERIGLDMLEEPEFATGTLNLYLSSEGADDERGRLRI
                     ADGLLAGPDLRLEISESAFEPHQLQIDGRLMFNPQPLRRTKVGKKLGKLTKLVQDKAT
                     GIPYIDLKVQGSWDKPNLIGKVIMDRLKKRGKRNFIRNIFGGHRPHKASVQELKDWFP
                     GWEP"
     gene            4044..5063
                     /locus_tag="PKO06_02295"
     CDS             4044..5063
                     /locus_tag="PKO06_02295"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HNV68499.1"
                     /translation="MRIRIWALRLICVWLLWGLSTALTWAAVPKVLYKSFFPISESQW
                     IIQGKMIYLEDDDAKEELKDRAFGNFFSHRCKQFPLTIALGSDTWRTKTDDGGNFRLE
                     AVATYAITASGTREFVFTSDTRSGWKETFSHDGTASAAFLIISDVDDTVLVTEVTNRF
                     KMLMKTFFTDPDRRGVVAGTPQVYQSLVHGTEPDKQGLLVFVSASPMYLAPRLEAFFL
                     HQKFPPFTLILREFGVKEKWEDKAEDLSDYKFTKISDLLNRTASMPVILLGDSGERDP
                     EIYARIVDAFPGRARCVIIRNITEQDEFHQRYLELKKKVKLIVWRDPQKLLAGLITEG
                     LVRAP"
     gene            5060..6241
                     /locus_tag="PKO06_02300"
     CDS             5060..6241
                     /locus_tag="PKO06_02300"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV68500.1"
                     /translation="MSLRRANRYISLAAKNSSLGVLRLWGLVLSLVVLPMVSKAAELK
                     LPAQGVGSGLFVAELLLSASESEAVEIRYDQTQLQYAGSVQPNPDIQLRYDGVVEIAS
                     GSGERRYQFKYLLLPPHQSSVLTLHTRHGTNTVTVSRTATEAAATYHLHLLAIALLLL
                     GVGVFLWKYQSRQVDLMSTRSLFYTYEELERLRREHETPAAPSVKETVPESTGPDARA
                     EAPRVVAPEPPIVGAPLPSGSQEPAPITGPLVEHQKSVSEPAASEAGVSISPIKTAST
                     GKESSPSLGASSESPTVMQYLNPFQVSLTDAQGRRFSGSGTEIIIGRRKDCQVVLTAA
                     EVSRTHACIRRQGRLFELVPLAPTNLTCVNEVIVTQPVLLKSGDTISLGGAGFRVTID
                     E"
     gene            complement(6284..>7028)
                     /locus_tag="PKO06_02305"
     CDS             complement(6284..>7028)
                     /locus_tag="PKO06_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68501.1"
                     /translation="RHLDGRTIPASLTRPASGPVRVHAGDPLGQVVPWIASVTPEQRR
                     YDHIHLEVVDWQGHFLNPNQFLPRPTDTLPPVIQQLWLLPNGSDNPFRVEEPEKLPVV
                     HGTIDLVIDTYDQLDGSLYRHVPYEVTWKIEAASPTPPVEFPERVAYRFDRIPRADDR
                     TVFADTFFQARIKGEPTVITCEGNNVGRRFLLHLAHGDVTSGYDAARGFDTRSLADGR
                     YVLVVTVRDIDGNATSKRETFEVRQVGSR"
BASE COUNT         1395 a         1995 c         2171 g         1467 t
ORIGIN      
        1 gcaggggagg ccgatctggg agtttcatga tctcccgacc gtgccgatgg gctcgatgtc
       61 ttcctcccca cgccccgcat ctggaaagac ggaacgaatc gacgacgaag tcctgaaaca
      121 gattcagatc cgtaacttgt ccgttcagat tccacgaccg cgtgactttc tcccggatca
      181 gacgctggta cgactcctgc gcctgccacc cggcaacatt gatgtaccgg gagtcgatgt
      241 gacgatcgaa cagcatcttc cggcggtgac cggccagcat tctgaaccga cgattgatca
      301 cacgattcgt gtggtgccgc ggcaggcgag cggcaccgtt cgaccgctga tctttggtct
      361 gttcagccgc gctcgtcaaa atgacgactg gaactgtgcc gttgaggtgc tggggcagcg
      421 tctggctctc ggtgtgcagc ggcagggaga acattcgcgg cgctatacca ttgtcgacga
      481 gcgaaaccgt gaccgtttct atcttcgctt cagtctgaac caggacaata cggtgtctca
      541 ctctcgaatg accgggttga acgggtcgat cgatgtgccc gaacttcgtc gtgttgccga
      601 gtggttgcgt ctgaatctcg atgttggcgg agcgctgcgg ctcacgctga aatcagaaga
      661 cacggccgaa ctcatcgata cgcagatgca tctcgaagct caatcactgc gcctgatgtt
      721 gttgccagcg ctgagtctcg agaatggaat catttcctgt cagttgcaga agcgcgaagg
      781 aacggtgact aacctggacg gaacctggtt cggccggcgc gtcgagcagc gcggagtctt
      841 tcaacttgca tcgcacggag gcgcaaccgc cagtttctcc gctcgtgttg ccgaagaacc
      901 gcttcgctgg acactgcaac tcgagccgca acgcgatcgc gctcgacgca tcgccatcga
      961 agtcgcgagg agtgccgggg agctgcggat cgctgccacg cagaatgctt ccggatcgaa
     1021 cgctcgatgg cgtgatggtg atctggaact gcgcgttcgt gacgggcaac gcatcacgga
     1081 tctgattccg tcaccgctga ttccctcacg atttgccgcg acgtgggcca gcatgaccac
     1141 gaccgtcgat ctcctcggcc cgcttcacgt gaccgggcag atcctctcgg atcagcagtt
     1201 tgaccaggca agagttgagt tgagactcga cggagcttcg atgcgcgacc ccagacagaa
     1261 cgacagtcga ctcgtgttgc agggccgtat tctcgtcgcc agccgctcgc tggagttgcg
     1321 tgaggtcggc ctgcgtcttg atcgtctgag cctcatcgct tcagggcttg tcgatctggg
     1381 cctgaacggc tatgtctggc aacgcgggag ggtggacgtt cgcggagagc tcaccggtac
     1441 cggagactgg gacctcgatg ccaggcttct gcaacgactc ttcgcgctgt cttctccacc
     1501 gggttttgaa cgcttgcgcg ttcgcggtca gcgactgctg tcgctttctg tcggtccaga
     1561 cgttggcgtg ccgaccttga cgctgctggc cgagaaggcg cttctcaccc gcggaaaagc
     1621 gatctggaac tgtgaacccc tgaatgtgcg ggtgttttct cccacaccct tccggtttga
     1681 aaaacctctg cccccccttc tgcagacaga acttgcaggt ggaatcttcg gggcgcgctg
     1741 tctcgccagt gccgcaatcg atctggaccg ccgcgagtgg ctgcagatag ggatgagcgc
     1801 ggacggtccg gatctggcgg ccattcagcg aactctcgcg gacgttcccg aggtggggtc
     1861 gtatctgcgc aaacatcaac tgacgcttgg cggcagcttt caactgcggg tctcaggaaa
     1921 agggcctctt gcctggccgg tcatgcaggg cgagtggcgg tttccggccc tggcgatcgc
     1981 ggtaccggga ttgcaggcgc aggtgccatg tcagatcgcc atcgaaacgc cggctcccgg
     2041 aacctaccgc ggcctggtga aaacctccaa tacgaagatc gaacgccgcg gagtgccgtt
     2101 cgtcctcgat tctctcaagg caacctggca gtatgagcgg gtgcccacgc cacgtcatca
     2161 cctgttgacg ctggcactcg acggaaagct cttcgatacc gaactgcggg gcaccataca
     2221 gtatctgccg cgtctgcggc gggtacaaca ggctcgcctg cagttcgagg gacggaatct
     2281 cgagaaactc tcacgtgagg tgggacgagt cgggcagttc gctttcccgt tcaccatgcg
     2341 cggcggcatt cgcggagagt gggttgcctc aggaacgctg cccgacctga ccagccgtgc
     2401 cacttgcacc tggaacggca tcgaagtgca ggtgccgatt cggcaatcag acaagccgct
     2461 ggtgtctctc gatgttcgcg acctgtcagg gaccctcgat ctggtgcaga acgcgcgcga
     2521 tcgcttcgac gtcgaactaa gcagtggcac ggcccgctgg ctgaatgcgc cgcttcgtct
     2581 ttcgggactg ggacatttgg cgcgtcaaga gtctggtcgc tggattcccg tgactgatcg
     2641 cctcgaactc gatctggcag atctgccgat tcgcgacgct gtagctttct ggaaacacac
     2701 cgctggcgca cgcgacgctc tgccggatgt caccgatctt tccggaacgg tttcgactcg
     2761 tgtcaggtta tcaggtctgc gaaatcgtta ccttggcgag ggaagcctga tactcaaagc
     2821 ggcggcgttc cgtctgccgg gagtggcgct tccgctcagc gagctcaatg gtacgattgc
     2881 gctcgggcgt cgaaccgcct cagaagccgt gtctctgtca gtcgatggtt tgcgcgggcg
     2941 cttcggacga gccgacgtgc gtattcctcg tggccgagtg atcgacccgt tgggaagctg
     3001 tcgaattgcc ttcgacggag tcgtctcgaa cattctgccg ggagacctgc tttctctgtg
     3061 cggaggctgg cgactccccg cgctctcgtt cccgcgcgag ggatcctatg ccggaacatt
     3121 gaacctgtca gggactgccg cttccccgaa ggcgagagtg agtgttacgg gcgacgagaa
     3181 gactgagatt cttttgcgga cgccgcagcg cgaataccgc ctgccggtcg gcccgtgtca
     3241 gatctctttt gcgtacgagc cgacaaatgg gaagatgagc gtggatcctg ggcagatcgg
     3301 ggttcttgat ggcttgatac gcattgatgc cgcgtcggcc gaactgcgct cgggtgcggc
     3361 gcgacagttc gaggtgcgcg gcaccttttc ggacattgat ctgggtaggt tctcgcgcga
     3421 cggtgagagc ctcgtgcgtg gtactctggt tggcacgttt cgagccaatc agacgccgga
     3481 ccgccatcgg gagctgctcg tgcatctcgg tatggaaaag ttgaagatcc ttaccttgcc
     3541 cgtcgatcag acgctcatgg aacgaatcgg tctcgacatg ctggaggagc ccgagtttgc
     3601 cacgggaacg ctgaacctgt atctttcgag cgagggtgct gatgatgagc ggggaagact
     3661 gcgcattgcc gacggacttc tggccgggcc cgatctgcgg ctcgagatct cggaaagtgc
     3721 ctttgaacct catcaactgc agatcgacgg tcgtttgatg ttcaaccctc agccgttgag
     3781 gcgcacgaaa gtcgggaaaa agctcgggaa actgacaaaa ctggtacagg acaaggcgac
     3841 aggaattcct tacatcgatc tcaaggtgca ggggagctgg gacaaaccaa acctgatcgg
     3901 aaaggtgatc atggatcgac tgaagaagcg gggcaagcga aacttcattc gcaacatctt
     3961 cggtggacac cggccgcaca aggcgagtgt ccaggagctc aaagattggt ttcctggctg
     4021 ggagccataa ttggaggaag gatatgagaa tacgaatctg ggcgctgcgc ctgatctgtg
     4081 tctggttact ctggggcctg tccaccgctc tgacgtgggc agctgttccg aaagtgttgt
     4141 acaagtcatt cttcccgata agcgaatcgc agtggattat tcagggaaag atgatctatc
     4201 tcgaggacga cgatgccaag gaagaactca aggatcgagc cttcggcaat tttttctcgc
     4261 atcgctgcaa acagtttccg ttgaccatcg cgcttggctc tgacacctgg cgcacgaaga
     4321 ccgatgacgg cggcaatttc cgactcgagg ccgtggccac atacgccatc acggccagcg
     4381 gtacgcgtga attcgtgttc acttctgaca cccggtcggg ctggaaagag acgttttcgc
     4441 acgacggtac cgccagcgcg gcgtttctga tcatcagcga tgtcgacgat acggtgctgg
     4501 tcaccgaggt aaccaaccgg ttcaagatgc tgatgaaaac gttttttacc gacccggacc
     4561 gacggggcgt cgtcgccgga actccccaag tgtaccagtc gctggtgcac ggaacggagc
     4621 ccgacaagca ggggttgctg gtattcgttt ccgcctcgcc catgtacctg gcaccacggc
     4681 tcgaagcttt cttcttgcat cagaagtttc cccctttcac cttgatcctg cgcgaattcg
     4741 gggtcaagga aaaatgggag gataaagcgg aagacttgag cgactacaag ttcacaaaga
     4801 tctcggatct gctgaaccgc actgcctcga tgccggtgat tcttctcggt gattcgggag
     4861 agcgagatcc ggaaatctat gccaggatcg tcgatgcgtt tccgggacgc gctcgctgcg
     4921 tcatcatcag aaacatcacg gagcaggatg aatttcacca aaggtatctc gagctgaaga
     4981 aaaaagtgaa gctgatcgtc tggcgcgatc cgcagaaact tctcgcgggg ctgatcaccg
     5041 agggactggt tcgcgcgccg tgagtcttcg acgcgcgaac cgctatatct ctcttgcagc
     5101 gaagaactct tctctaggcg tgttacgcct ctgggggctg gtactgtcgc ttgtcgttct
     5161 ccccatggtc agtaaggccg ccgagttgaa actgccggcg cagggagttg gaagcggtct
     5221 ctttgtcgcg gaactgctgt tgtcggcctc tgagtcagag gcggttgaga ttcgatacga
     5281 tcagactcag ctgcaatacg cgggttctgt gcaacccaac ccggacatac agctgcgata
     5341 tgacggagtg gttgagattg cctctgggtc tggagagcga cggtatcagt tcaagtatct
     5401 gctgctgcca ccccatcaga gttcggtgct gacactgcac actcgtcacg gcacgaacac
     5461 ggtgaccgtc tcacgcacgg ccacggaagc cgcggcgacc tatcacctgc atctgctcgc
     5521 gatcgctctc ctgctgctgg gcgtcggggt gtttctctgg aaatatcaga gccggcaggt
     5581 cgatctgatg tcgacgcgct ccctgttcta tacgtatgaa gaactcgaac gcctgcgccg
     5641 agaacacgag acgcccgctg caccatcggt gaaggaaacg gtgccggagt cgaccggacc
     5701 ggatgccaga gccgaagctc cacgcgtcgt cgcacctgag ccgccgatcg tgggagcccc
     5761 attgccgtcc ggttcccagg aacctgcacc gataaccggg cctctggttg aacatcagaa
     5821 gtcggtgtct gaacctgcgg cgtccgaggc aggcgtgtcg ataagcccca taaaaacggc
     5881 atcaaccggg aaagaatcgt cgccatctct gggtgcttcg agtgagtcgc cgacggtgat
     5941 gcagtatctc aacccttttc aggtgtctct gaccgatgcg cagggacgca ggttcagtgg
     6001 gagcggcacg gaaatcatca tcggtcgccg caaggattgt caggttgttc tgacggctgc
     6061 ggaagtgagc cgcacacacg cctgtattcg ccgtcagggc cggttgtttg agttggttcc
     6121 gctcgcgccc accaacctga cgtgtgtgaa tgaggtgatc gtaacccagc ctgtgttgct
     6181 aaagagcgga gatacgatct ccctcggcgg agcaggtttt cgcgtgacga tcgatgaata
     6241 gggctttcgc caggcacgaa gaaggtgaga ctcccgaaaa cgcttaacgc gagccgacct
     6301 gtcgaacctc aaaagtctca cgtttcgaag tggcgtttcc atcaatgtct cgaacggtaa
     6361 caaccaggac ataccgacca tcggcgagcg acctggtgtc gaaccctctg gccgcatcat
     6421 acccgctggt gacatcgcca tgagcgaggt gcagcaggaa gcggcgtccc acgttgttcc
     6481 cttcgcaggt gattacggtg ggctcgcctt tgatgcgggc ctggaagaat gtatcggcga
     6541 acaccgtccg gtcatcggcg cgaggaattc gatcaaaacg ataggcaacg cgttctggga
     6601 attcgacagg cggggtgggg gaagctgctt cgattttcca ggtgacttca tacggaacat
     6661 gccggtagag agagccgtca agctgatcgt aggtgtcgat gaccaggtca atcgtgccat
     6721 ggaccaccgg cagcttctcg ggctcctcga cgcgaaatgg gttgtcagag ccgtttggca
     6781 gcagccagag ttgttgaatg accggaggca gggtgtcggt tggccgtggc agaaactggt
     6841 tcgggttcag aaaatgcccc tgccagtcga cgacttcgag gtgaatatga tcataccgtc
     6901 gctgttcggg ggtcacagag gcgatccagg ggacgacctg gccgaggggg tcgccggcat
     6961 gcactcgcac cggccctgag gcaggccggg tgagcgaagc cggaatcgtc ctgccgtcca
     7021 gatggcgg
//
LOCUS       DAORHD010000061         7016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10536_length_7016_cov_4.529665, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000061 DAORHD010000000
VERSION     DAORHD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10536_length_7016_cov_4.529665"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKO06_02310"
     CDS             <1..653
                     /locus_tag="PKO06_02310"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68502.1"
                     /translation="SFNQMARKLEERRLIRMLVSRVARDLAATGPSLEAGYRDVTILF
                     LRSRVSESTPRKAGELFQQLRLQLSEISRLSSRHGGDIDKVMGEKILIVFSHHAGAAD
                     AAERARRLIDDLIQAGRDRRLPIPWTGGLHAGKVISGLLGAGNRQDMTVIGDPVNAAA
                     RLESLTHHLSQDAVIASDEVVQWLADRSAFVAHGTVQVKGKRQPLTIYVKSTAIQT"
     gene            complement(691..1314)
                     /locus_tag="PKO06_02315"
     CDS             complement(691..1314)
                     /locus_tag="PKO06_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV68503.1"
                     /translation="MLFEQIPVGGMGNFAYLFADREGGEACLVDPAHQAEKLLARVKE
                     LKLNVTRVLLTHHHFDHLNAAMTLKARTGAKLLAHAETARLAAGSVSVDDHLSDGDSF
                     ILGGETVHVLHTPGHAPGSLCFVVGGQWLITGDTLFVGNCGRTDLPGGDPRQLFASLQ
                     RLKQLPDSLVICSGHDYGLAKTRTLGEEKKLSAVLKAATFAEFDAIP"
     gene            1410..2072
                     /locus_tag="PKO06_02320"
     CDS             1410..2072
                     /locus_tag="PKO06_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNV68504.1"
                     /translation="MNLVECAIMNPITHALVGWTIARPLSSHRRDRLMITLAGILPDI
                     DGLGLPVEALTNGTHDWYSTWHHALTHNLTAAAALAAILTFIRRWGERRQTSPQDDAR
                     PPLGSIWLFLACLLSTHLHLLGDLIGSKGPDGDPWPVPYFAPFSDVTWVAPFQWEINA
                     WPNFALTILLLTWAFRCAWRDGESPIGFISTRADQSFVETLRQRFGNPTSIATDDIRP
                     SC"
     gene            2082..3194
                     /locus_tag="PKO06_02325"
     CDS             2082..3194
                     /locus_tag="PKO06_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HNV68505.1"
                     /translation="MSDNQHLGLYIHVPFCRSKCSYCGFFSLPFAAELCELWLQHLFV
                     EFDRRIEAEKIQRVRTAFIGGGNPTVLPLAQFTAFVDGLQRRLEPSPIKEWTFETNPE
                     TFTREHLAVLRSVSGLRLSFGLQRLRDDELTRIGRQAHASCGLRALEQAFSTITNVGA
                     DFILGVPGLPSLAGDLDLLLHRFPLTHVSTYFLTAEPNTPLAREIENGRFPDPAECGP
                     EELFEVRAALERHGLQQYEISNFALPGRECLHNLHYWYQGDYIGIGPAAVSTLDGIRT
                     ANPEGLEQWINGEHQIERLSPIDQRREYLMLRLRLLREGFSPIDYASRFGIGVNELVQ
                     QLDTLVDRGDLTRTPEGYALSISGMSVANRIISELF"
     gene            complement(3150..5339)
                     /locus_tag="PKO06_02330"
     CDS             complement(3150..5339)
                     /locus_tag="PKO06_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68506.1"
                     /translation="MNIREVLDLTVLGPLLESNWRLTGVPVRLFLMNGEKSAELGKPS
                     LCSRFHEQHPTTRQVCLKAEFLRNIESFTDWNCPFACGNGLREVALPVWLEEKRVGVL
                     FLGPFWLESEELPLEEFRQRARTYGFDEASYVEALRALPVLSHERLERVKAFYADFLN
                     VVSHLGLRNLQWKREAVERNRSQLLAAESEAKYRMLVEGSSDLIYSIGIDGCFTFLSA
                     GVEKFGYTPEEVLGKPMFSFVHPDDRQRIIDKYREGFRRGAHPTTNEFRVITKDGGSR
                     NVEEAGRIICDEQGKPVMVTGILRDVTQRTIAEDALRRGKEIAEAANRAKSSFLAAMS
                     HEIRTPMNAILGFSDLVMQSPLPDDLRSQMEIIRKSGHDLLGIIDDLLDLARIEADQI
                     RFEEKPFSLPQVLREVRQALDVRVREKGLRLVVEAPEQLPDQWLGDALRLKQILHNLI
                     GNAIKFTREGAITLGVQVLPEPSSGESPREIHLTVTDTGIGIPEDKLQTIFEPFTQVD
                     GGYSRRYGGIGLGLAICRRLLEHLGGSIRVESRLGVGSTFTCRIPLTCLGSECPAQVT
                     PMVTPMVATMSRMLRILLAEDEPMSSLLAQTILSRQGHQVTAVETGRDALAAIEGQPF
                     DVVLMDVSMPEMDGLEATRRIRAWENRAVSLEGVAARRSHVPIIALTAHAMKGDEERI
                     RASGVDAYLTKPVVPERLLALVTQLAGSRSDRRSEELRDDPVRDAHS"
     gene            complement(5421..6776)
                     /locus_tag="PKO06_02335"
     CDS             complement(5421..6776)
                     /locus_tag="PKO06_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNV68507.1"
                     /translation="MKPLVNPRRVRSLADGRSGAGPVVYWMQRDQRAEDNWALLAAWQ
                     QAVERQRPLLVVFCLVSDFLEATMRQFSFLLKGLEETSATLTERGIAFTVLTGAPAKV
                     LPAFLREHDAACLYTDFNPLRLCRTWKAEVLARISIPAFEVDAHNVVPAWVASPKLEV
                     GARTLRPKIHRLLPEFLEEFPRRLPDVLPWKQKLSSFDWDDCRTSLKVRRDIGELTHW
                     VPGTHAALSQLKTFASSMISSYDTGRNDPNVRAQSDLSPYLHFGQIAPQRVALVAARA
                     AVTEESCNAFLEELIVRRELADNFCLYEPRYDSVDAFPRWARESLQDHLKDPRPHRYT
                     LEQFERAETHDALWNAAQRQMVNHGKMHGYLRMYWGKKILEWCREPHEALAIANLLND
                     RYELDGRDPNGYAGTAWSIGGVHDRAWPTRPIFGKVRYMNLNGCRGKFDVDAFVNVWG
                     T"
     gene            complement(6760..>7016)
                     /locus_tag="PKO06_02340"
     CDS             complement(6760..>7016)
                     /locus_tag="PKO06_02340"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNV68508.1"
                     /translation="LLMLLFGRWQMGTGLRFAFFLGWYGTSRFFVEFIRLNKEFWNHL
                     TSDQLLAAGLVVASVIFTIAVPRSGGEISPPEPQKHETPG"
BASE COUNT         1446 a         2186 c         1946 g         1438 t
ORIGIN      
        1 tctctttcaa ccagatggct cgcaagctgg aagagcgtcg cctgatccga atgctggtca
       61 gtcgggtcgc ccgggatctt gcggcaacag gcccgtctct ggaagccggg tatcgcgacg
      121 tcacgattct gtttcttcgg tcacgggtct cggagagtac tccccgcaag gcgggtgagc
      181 tctttcaaca gttgcgcctg cagttgagcg agatcagtcg tctgtcctcg aggcatggcg
      241 gcgacatcga caaggtgatg ggcgaaaaga ttctcatcgt cttttcccat cacgcaggcg
      301 cagctgatgc cgccgaacga gcccgtcgcc tgatcgatga cctgatccag gccggtcgcg
      361 atcggcgact tccgataccc tggaccggcg gactccacgc cgggaaagtc atatcaggcc
      421 tgcttggcgc aggcaaccgt caggatatga ccgtgatcgg cgacccggtg aatgccgctg
      481 ccagacttga aagcctgacg caccacctga gtcaagacgc ggtcattgct tcagacgagg
      541 tggtacagtg gctcgcagac cgctccgcct tcgtcgccca cggaactgta caagtgaaag
      601 gaaagagaca acctctgaca atttatgtga aaagcacagc tattcaaacc tgaacggatc
      661 gtcatccgct catcaagacc gcgcaccagg ttacggaatg gcatcaaact cggcgaacgt
      721 cgctgccttc aggaccgcgc tcagcttttt ttcttccccg agagtacgag tcttggcaag
      781 gccataatca tgaccagagc agatcacaag gctgtcggga agttgtttga gacgttgcag
      841 actggcaaac agctggcgcg ggtcaccacc cggcagatcg gttcgaccgc agttgccgac
      901 gaacagagta tcgccagtga tcaaccactg cccaccgacc acgaagcaaa gacttccggg
      961 agcgtgaccc ggagtgtgca atacatgcac ggtttcacca ccaaggatga atgagtcgcc
     1021 atcggacagg tgatcgtcga ctgagacgct gccagccgcc agacgagcgg tttccgcgtg
     1081 ggccagcagc ttcgcccccg tgcgggcctt caatgtcatg gccgcattga ggtgatcgaa
     1141 gtggtgatgc gtcagcagaa cgcgcgtcac attgagcttc agctctttca cgcgcgccag
     1201 aagtttctcg gcctggtgag ccgggtcgac cagacacgcc tctcccccct cgcgatcggc
     1261 gaacagatac gcgaaattgc ccattccccc cacgggaatc tgctcgaaca gcatgtcaca
     1321 cacctcctgt tcacatccgt gaacgtcacg gtttttctac ggtactccat tttcgcaacc
     1381 gagaaaagcg ggcggaattt caaggcagaa tgaatctggt agaatgcgcc atcatgaacc
     1441 cgatcactca cgccctggtc ggctggacaa tcgcacgacc gctgtcgtcg catcgtcgcg
     1501 atcggctgat gatcaccctt gccggcatcc tgcccgatat cgacgggttg ggtttgcctg
     1561 tcgaagcgct gacgaacggc actcatgact ggtattccac ctggcatcat gccctgacgc
     1621 ataatctcac ggccgccgcc gcgctcgctg ccattctgac gttcatccgt cgatggggag
     1681 agcgacgcca aacctcaccc caagacgatg cccgaccacc gctcggttcg atctggttgt
     1741 ttctggcttg tctgctctcg actcatctgc atctgctggg cgacctgatc ggttcgaaag
     1801 ggcccgatgg agacccgtgg ccggttccgt acttcgcccc gttttccgac gtcacctggg
     1861 tggcgccgtt tcagtgggaa atcaatgcct ggccgaattt cgcgctgacc attctgctgc
     1921 tgacgtgggc ttttcgctgc gcctggcgcg atggggaatc accgatcggg ttcatttcaa
     1981 cgcgagccga tcagtcgttc gtcgagacac tgcgtcagag attcggaaac cccacttcca
     2041 tcgcgaccga cgacattcgt ccgagttgct gagcagctat cgtgtctgac aatcaacatc
     2101 ttggcctcta tattcacgtg cctttctgtc gttcaaaatg ttcttactgc ggtttcttct
     2161 ctctgccatt tgccgcggaa ctctgcgaac tctggctgca gcacctgttc gttgaatttg
     2221 accgacgcat cgaggcagag aagatccagc gagtgcgaac cgctttcatc gggggtggga
     2281 atccgacggt gctgccgctc gcccagttca cggcgttcgt tgacggcttg caacgtcgac
     2341 tggaaccaag ccccatcaag gaatggacct tcgagacgaa ccccgaaacc ttcacccgcg
     2401 aacatctggc cgttcttcga tccgtttccg gtctgcgtct gagcttcgga ttgcagcgac
     2461 tgcgtgatga tgaactgaca cgcatcggac gtcaggcgca tgccagttgc ggtcttcgag
     2521 cgttagagca agcgttcagc accatcacca acgtcggtgc cgatttcatt ctgggagtgc
     2581 ctggtctgcc ctcgctggcg ggtgatctcg acctgctgct gcaccgtttt ccgcttaccc
     2641 atgtgtcaac ctactttctg accgctgaac cgaacacccc tctggctcga gagatcgaaa
     2701 acggacgctt tcccgatcct gccgaatgcg gtccggaaga actcttcgaa gtcagagcgg
     2761 cactcgagcg tcacgggttg cagcagtacg agatttccaa ctttgcgctt ccgggtcgcg
     2821 agtgtctgca taatctgcac tattggtacc agggggatta cattggtatc ggccccgctg
     2881 cggtttccac gcttgacggg attcgaaccg cgaatcccga aggtctggaa cagtggatca
     2941 acggcgagca tcagatcgag cgactcagcc cgatcgatca gcggcgggaa tatctcatgt
     3001 tgcgcctgag attgctgcgc gaaggttttt cacccatcga ttatgcttcc cgattcggca
     3061 tcggggtcaa tgaacttgta caacagctcg acacactcgt cgatcgggga gatctgactc
     3121 gcacccctga gggctatgcg ttgtcgatct caggaatgag cgtcgcgaac cggatcatct
     3181 cggagctctt ctgagcgccg gtcactccgg ctgcccgcca gttgcgtgac cagtgccagc
     3241 aggcgttcgg gcaccaccgg cttggtcaga taggcatcaa ctcccgaggc gcgaatgcgt
     3301 tcttcgtcgc ccttcatcgc atgagccgtc agggcgataa tcggcacgtg cgagcgacgc
     3361 gccgccaccc cctcaagaga cacggcccga ttttcccagg cccgaatgcg tctcgttgcc
     3421 tcgaggccgt ccatttccgg catcgacaca tccatcagca ccacgtcaaa cggctggccc
     3481 tcgatcgccg caagagcatc tcgacccgtc tcgaccgcgg tcacctgatg cccctgccgg
     3541 gaaagaatcg tctgagccag cagactgctc atcggctcat cttccgccag cagaatgcgc
     3601 aacatgcgcg acatcgtggc aaccatcggc gtgaccatcg gagtcacctg cgccggacat
     3661 tcactgccga gacaggtcag cggaatgcgg cacgtaaacg tactacccac gcccagtcgg
     3721 ctttcgacgc ggatactccc ccccaggtgc tcgagcagtc ggcggcagat ggcaaggccg
     3781 aggccgatac ctccgtaccg gcgcgaatac ccgccgtcaa cctgcgtgaa cggctcaaag
     3841 atggtctgca gcttgtcttc cggaattccg atgccggtgt cggtcaccgt caggtgaatc
     3901 tcgcgcggac tctcaccgct cgagggttcg ggcagaacct gaacgccgag ggtaatcgcc
     3961 ccttcgcggg taaacttgat cgcattgcca atgagattgt gaagtatctg tttgaggcgc
     4021 agcgcgtctc ccagccattg atcaggcagt tgctccggag cctcgacgac gagacgcagc
     4081 cctttttcgc gcacccgcac atcgagagcc tgccgcacct cgcgcaaaac ctgcggcaac
     4141 gagaagggct tttcctcgaa gcggatctga tcagcctcga ttcgcgccag atcgagcaga
     4201 tcgtcgatga tgccaagcag atcatgcccg cttttcctga tgatttccat ctgcgaccgg
     4261 agatcgtctg gcaatgggct ctgcatgacc agatcagaga atccaagaat cgcattcatc
     4321 ggggttcgaa tctcgtgact catcgccgcg agaaacgaac ttttcgcgcg gttggcggcc
     4381 tcggcgatct ctttgccgcg acgtaacgcg tcttcggcga tagttctctg cgtcacatca
     4441 cgcagaatgc ctgttaccat cacgggttta ccctgttcgt cacagatgat tcgccccgct
     4501 tcctcgacgt ttctgcttcc tccatccttg gtgatcacac gaaactcgtt ggtggtcggg
     4561 tgcgcacccc ggcgaaatcc ttcgcgatac ttgtcgatga tacgctgtcg atcgtccggg
     4621 tgcacaaagg aaaacatcgg cttgccgagc acttcttcgg gggtataccc aaacttttcc
     4681 accccggcgc tcagaaaggt gaaacaaccg tcgatgccaa tcgagtagat gagatcagaa
     4741 ctcccctcga cgagcatgcg atacttcgcc tcgctctcgg cggccagcag ctgcgagcgg
     4801 ttgcgttcga cagcctcgcg cttccactgc agattgcgca ggcccagatg cgagacgacg
     4861 ttcaagaaat ccgcgtagaa cgccttcacc cgctcgagac gctcatggct gagcaccggc
     4921 agtgcccgca gcgcctccac gtacgacgct tcatcgaatc cgtaagttcg agcccgctga
     4981 cggaattcct cgagcggcaa ctcctccgat tcgagccaaa acggcccaag aaagagcact
     5041 cccacacgct tttcttcgag ccacaccgga agggccacct cacgcagacc gttgccacag
     5101 gcgaacgggc agttccagtc ggtaaatgac tcgatgttgc gcaggaattc cgctttcaga
     5161 cagacctggc gagtcgtggg gtgctgttca tgaaaccgcg agcagagcga gggtttgcca
     5221 agttcggctg acttctcgcc gttcatcaga aacagacgca cgggaacccc ggtcaggcgc
     5281 cagttgcttt ccagcaatgg ccccaaaacg gtgagatcca gtacttctct gatgttcatg
     5341 tcctgcgcac ccgatcctgc tgttctctgt tgtgttttct gattatactc acgaaagaca
     5401 caacccggaa taccccgacg tcaggttccc cacacattca cgaaggcatc gacgtcgaac
     5461 ttgccacgac agccgttgag gttcatgtag cgcaccttgc cgaaaatcgg acgcgtcggc
     5521 caggcgcgat cgtgcactcc cccgatcgac caggcggtgc ccgcataccc gttcggatca
     5581 cgaccatcga gttcataccg atcgttgagc aggttggcga tcgcgagcgc ttcgtgcggc
     5641 tcccgacacc attccagaat cttctttccc caatacatgc gcagatagcc gtgcatcttg
     5701 ccgtggttga ccatttgcct ctgagcggca ttccagagag catcgtgcgt ttcagcccgt
     5761 tcgaactgct cgagcgtgta tcggtgtgga cgcggatctt tcaggtggtc ctgcagcgat
     5821 tcacgagccc agcgcggaaa tgcgtcgacg gaatcgtacc ggggctcata gaggcagaag
     5881 ttgtctgcca gctcgcgccg cacgatcagt tcttcgagaa acgcgttgca cgactcttcg
     5941 gtaaccgcgg cccgggcggc aaccagtgcc acgcgttgcg gcgcgatctg gccgaaatgc
     6001 agatacggag acaggtcgga ttgcgctcgg acgtttggat catttcgacc cgtatcatag
     6061 ctgcttatca tcgaagaagc gaatgtcttc aactgggaca gagccgcgtg tgttccggga
     6121 acccagtgag tgagctcacc gatatcccgt ctgactttga ggctggtgcg acaatcgtcc
     6181 cagtcgaacg agcttaactt ctgcttccaa ggcagcacat caggtaatcg acgtggaaac
     6241 tcctcgagga attccgggag caggcgatgg attttcgggc gcagcgtacg agcgccgact
     6301 tcgagcttcg gcgaagccac ccacgccggc accacgttgt gcgcatcgac ctcgaaggcg
     6361 ggtatcgaaa tccgcgccag cacttccgct ttccatgtgc gacacagccg caacggatta
     6421 aagtcggtat agaggcaggc tgcatcgtgc tcacgcagaa aagccggcaa cactttcgcc
     6481 ggtgctccgg tcagcaccgt aaaggcaatt cctctttcgg tcagggtcgc cgatgtttcc
     6541 tcgagcccct tcaacaggaa gctgaactgg cgcatcgtcg cttccagaaa gtctgaaacc
     6601 aggcaaaaca cgaccagcaa cggccgctgc cgttccactg cctgctgcca ggcggccagc
     6661 aatgcccagt tgtcctcggc ccgctggtcg cgctgcatcc agtagaccac cggtcccgcc
     6721 cccgatcgac cgtcggccag agagcggaca cgccgggggt taaccagggg tttcatgctt
     6781 ctgtggttcg ggcggggaga tttcgccccc tgagcgcggc accgcaatgg taaaaatcac
     6841 cgacgcgacg accagccccg ccgccagcag ttggtcagac gtgagatgat tccagaattc
     6901 cttgttgagg cgaatgaatt cgacgaaaaa tcgcgacgtt ccataccagc cgaggaagaa
     6961 cgcgaagcgc agcccggtgc ccatctgcca gcgaccgaaa agcagcatca atagca
//
LOCUS       DAORHD010000062         6972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10634_length_6972_cov_3.300275, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000062 DAORHD010000000
VERSION     DAORHD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_6972_cov_3.300275"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKO06_02345"
     CDS             complement(<1..491)
                     /locus_tag="PKO06_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68509.1"
                     /translation="MDAATFLTQVRAGRLDGRVYLFAGRESFLKERALEQVKSRFVPP
                     DEQAESFHIIDFAHREASEFPALLHSFSFSSGRRVFALYQFDKLGAAERKQIYAVLKQ
                     AIPEDALVFLLTDETPVAAEAAKALAEVVQRLDFWPPFENQVPTWVRQEAADAGAQMT
                     PEAV"
     gene            complement(569..1999)
                     /locus_tag="PKO06_02350"
     CDS             complement(569..1999)
                     /locus_tag="PKO06_02350"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF002600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNV68510.1"
                     /translation="MAKKTAAKTKETSLKYQPVNAWDKLTPVQDKEMEQVAKRYIDFL
                     GTCKTERETLTYLEQHAKKHGFREFAPGRKLKAGDRLYWISKNRAMGMAIVGRRPIAD
                     GVRMLAAHHDVPHLDLKALPLYERHGLGLLRTHYYGGVKKFQWATIPLAIHGFACTKK
                     GKTLSFAIGEKADDPVFTILDIAPHLSYKIQNDRKATEVLRGEELQVVAGHRPQPLKK
                     GETKGEDRIKMMVLEYLQKHFGFEEEDLAWAEVAVVPAMPPREVGMDRSLIGGFGHDD
                     RACVFASFEALLGIKTPEHTAAALFFDKEEIGSAGASGAQSMMVTDFFACLVEAATGS
                     CDFSLLRRTMCNSQALSADTNAAIDPIFEGAYDSSNAGLVGRGVWVCKFTGSGGKAGS
                     SEADVEFVAQIRDLLTDAKIPYQFGEMGKVDEGGGGTVAKFLAQLNMHVLDIAIPVLS
                     LHSPFEVLSKVDLYYSVLAYRVFMAK"
     gene            2256..2390
                     /gene="rpmH"
                     /locus_tag="PKO06_02355"
     CDS             2256..2390
                     /gene="rpmH"
                     /locus_tag="PKO06_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNV68511.1"
                     /translation="MSFTYNPNKRKRQKTHGFRKRMATVSGRKVLKRRKLRGRKRICL
                     "
     gene            2381..2785
                     /gene="rnpA"
                     /locus_tag="PKO06_02360"
     CDS             2381..2785
                     /gene="rnpA"
                     /locus_tag="PKO06_02360"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNV68512.1"
                     /translation="MSVIRPGLHTLTTKAEFDRVFAERKRFSRDGIAFYYLVQRHGDT
                     RLGIMAPRRYGKAVKRNRFRRRLKEIFRLSPETPPADIVITVYKPYEELGFALLQRTV
                     RWGLDKIRRCQPPAGSTGQAAQVPAAPPQEDR"
     gene            2782..3033
                     /gene="yidD"
                     /locus_tag="PKO06_02365"
     CDS             2782..3033
                     /gene="yidD"
                     /locus_tag="PKO06_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV68513.1"
                     /translation="MTHHEEPALPAPGIASKASFVFIRWYQRWLSPYIGVHCRFEPTC
                     SRYTFAAIEKYGFIRGWLMGIYRIFRCNPFNPGGYDPVR"
     gene            3056..4663
                     /gene="yidC"
                     /locus_tag="PKO06_02370"
     CDS             3056..4663
                     /gene="yidC"
                     /locus_tag="PKO06_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV68514.1"
                     /translation="MTHSAPLSFSRRLAVLRALTVGLWLLAWLIPTPLSAQATGTLAP
                     IVAPAATQAVTTELRDRNADGKADVAVETPRLRLLISGATGNLEVFYLRGINFEENLY
                     PPVIAGHGYSFASLTMRPFALQINGSDEPDGTWTFDTPTVGTQSIVVTAKRSEPVSGL
                     ELTKTYRFTLNGYQFGYDVTVTNSTGKDMIVGTDALGGLNLMYGPGLFIDPFNPSSYL
                     GLHDGSHIQYDNVAKFREAAAKERFQGVGLKTTYFCMLMDAGIPVNLIAQGFEVKPTD
                     TKQTRNRTFEGQVVGVSIPPFTLKAGESRHVPFTLYYGPKLLDELALINRGNVTDYGF
                     LSTMLLRILQFFHGLFPNFGLAIIFLTLAVRAMLHPLTVKQTKSMAQMQKIQPMLQDL
                     KDRYRDNPQKFNEEVLKLYQKHNVNPLGGCLPLLLQLPILIALYNTINIAVELRKVPF
                     LWLTDLSKADPYIILPLAITALMYAQQGQTTDPQQQQMMAFMPMFMFIITWTLPSGLL
                     LYWFTSSVIGVVQQLQANKMVAKIKEE"
     gene            4670..5689
                     /gene="jag"
                     /locus_tag="PKO06_02375"
     CDS             4670..5689
                     /gene="jag"
                     /locus_tag="PKO06_02375"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNV68515.1"
                     /translation="MKRVIEITAKSHEEARALAQKEVRPGETITQSDILQEPTRGIFG
                     IVGNPDVRIRFTIDEIPAPPPVAPKVVTPATPSPRPEPEADFGDAEEATDLDEDLVDE
                     AEDIYVDEEIPADAEAHGGRKPRVYSGPTGTNHPDYEQLIRLVHQVAALVGSGEITVS
                     ERIDGDAWIIEPNGDNLTPFIGKHGRTLDAIQYLVNIVANKGREDKVKIVLDAQGYRD
                     SRHRRLISLAHRMCRKVADGGRAVELEPMSTVDRRTIHLALKDRTDIQTYSRGIEPMR
                     RVVIAPRKGAQAGGGRRGGQRPRRYGGPGRFGGRDQQGEHRTQGEQNGPRPVPMFMGE
                     DGDND"
     gene            5739..>6972
                     /gene="mnmE"
                     /locus_tag="PKO06_02380"
     CDS             5739..>6972
                     /gene="mnmE"
                     /locus_tag="PKO06_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNV68516.1"
                     /translation="MIRISGPETFAILKQVFRYNGKRAFPGPFAVFPGHLQDPGDGSA
                     IDQVLVTTFCSPASYTGEDMAEITLHGNPVLLSTALSALFQAGAHPAEPGEFIRRAFL
                     NGKLDLLEVEGVSQLLSAVTRTQAQIALHQLDGLPSQRIRTIRETLLERLTHLEATLN
                     FPEEAIDDLDQQQLAAPLRQATAELERFLAAARQGSLLAAGLRLVLIGKPNTGKSSLL
                     NRLIGRERAIVTEIPGTTRDTLEEIFSLGGLPVRLIDTAGLRNSDDRLEQLGIERTRE
                     AVAGAFFLIVLFDRSEPFTDEDHFTLELVRNSNKPFVMALSKGDLPDRFSHAPTEVTQ
                     ALVLSTRTGQGIQELTDLVARRLRELEPGSLDELMFLGAQQLASLAKALGMLHSLLQN
                     LDHLYQDLIAVELEGIIRE"
BASE COUNT         1468 a         2178 c         1873 g         1453 t
ORIGIN      
        1 accgcttcgg gggtcatctg cgctccggcg tcggcggctt cctgtcgcac ccacgtcggc
       61 acctgatttt cgaacggcgg ccagaaatcg agtcgctgca cgacctctgc cagagctttt
      121 gcggcttcag cggcaaccgg agtctcatcg gtcaacagaa acacgagagc atcttccgga
      181 attgcctgtt ttaacactgc gtagatctgc tttctttcag cagcccccag tttgtcaaac
      241 tggtacaacg caaacacccg tcgaccgctg gaaaacgaaa aactgtgtaa cagcgcggga
      301 aactcggatg cctcacgatg ggcgaagtca atgatatgga aactttctgc ctgctcgtcg
      361 ggcggaacga atcgcgactt gacctgttca agcgcgcgtt ccttgagaaa cgactcacgc
      421 cccgcgaaca gatagacccg accgtcaagt cgcccggctc gcacctgggt cagaaacgtc
      481 gccgcatcca tgttccgcct ccaaaagaga acgcggtcgc aacccaaggc tgttggttac
      541 gaccgcggtt ggtcaagcac tcatctgcct atttggccat gaacactcga taggccagca
      601 cggaatagta caagtcgact ttcgagagca cttcgaaggg ggaatgcaga ctcagcacag
      661 gaatcgcgat gtcgagcaca tgcatattca actgagccag gaacttcgcg acagttccac
      721 cgccgccttc atcgaccttt cccatttctc cgaactggta cggaatcttg gcatcggtca
      781 acagatcgcg aatctgagcc acaaactcga catccgcctc gctgcttccc gccttgccgc
      841 ccgaaccggt gaacttgcac acccaaacgc cacgaccgac gagaccggca ttcgaagagt
      901 catacgcacc ctcgaagatc gggtcaatcg ccgcgttggt atctgccgac agggcctgcg
      961 agttacacat ggtgcgacgc agcagagaaa agtcgcagct tccggttgcg gcttcgacca
     1021 ggcaggcaaa aaagtcggtg accatcatgg actgcgcacc gctcgccccg gcggaaccga
     1081 tttcttcctt gtcgaagaat agcgcggcag ccgtgtgttc cggggtcttg atgccgagaa
     1141 gagcttcaaa cgaagcgaac acgcaggcac gatcgtcatg accgaaacca ccgatcaggc
     1201 tgcggtccat gccgacttca cggggcggca tcgcgggcac gacggccact tcagcccacg
     1261 cgagatcttc ttcctcgaac ccgaagtgtt tctgcagata ctcgagcacc atcatcttga
     1321 tgcgatcttc acctttggtt tcgcccttct tgagcggctg cggacgatgc ccggcgacga
     1381 cctgcaactc ttcgccacgc agcacttcgg tggccttgcg gtcattctga atcttgtaag
     1441 acaggtgcgg agcaatatcg aggatcgtga acaccggatc atcggctttt tcaccgatag
     1501 cgaacgacag ggtcttgccc ttcttcgtgc aggcgaagcc atgaatggcc aaaggaatgg
     1561 tcgcccactg aaacttcttc actccgccgt agtagtgagt gcgcagcaaa cccagaccat
     1621 ggcgttcata caggggcaga gccttcagat cgagatgcgg cacatcgtga tgagcggcca
     1681 gcatgcgcac gccatccgcg atcggacgac ggccgacaat ggccataccc atcgcccggt
     1741 ttttgctgat ccagtagagg cgatcgccgg ctttcagctt gcgaccggga gcgaactcgc
     1801 ggaaaccgtg tttcttcgca tgctgttcca gataggtcag tgtctcgcgt tccgtcttgc
     1861 aggttcccag aaaatcaatg tagcgtttcg ccacctgttc catttctttg tcctgaacag
     1921 gggtcagttt gtcccaggca ttcaccggct ggtatttcag ggacgtttcc ttggttttcg
     1981 ccgcagtctt cttcgccatg tctctttcct ttccgtttct ctgcagagga agccctctgg
     2041 atatttttcg gctcgcatac tatcatattc ttcccggcga gaaaacagat tcgtgtaccg
     2101 tgtttttggc agaagggcat ccggttggca gtcacgcttc agacgaggtc gggcggggat
     2161 ggggtacaaa acccggcaat tgagttgaca ttcgctgtcc atctgtaata tactcgatcc
     2221 ctaatcccaa tcgttcatcg gacaggagta gagtcatgag ttttacgtat aatccgaata
     2281 aacgcaagcg ccagaagacc catggtttcc gcaagcgcat ggccaccgta tctggccgca
     2341 aagtgctcaa gcgccgcaaa cttcgcggtc gcaagcgcat ttgtctgtaa tccggcccgg
     2401 actacacacc cttaccacca aagccgagtt tgatcgcgtc tttgccgagc gaaaacgctt
     2461 ttccagagat ggaatagcgt tttattatct ggtacaaagg cacggagaca cgcgccttgg
     2521 catcatggcg ccgcgtcggt atggcaaggc agtcaagcgc aatcgatttc gtcgtcgatt
     2581 gaaagagatt tttcgcttat cccctgaaac gccgcctgcg gacatcgtga tcacggtgta
     2641 caaaccctac gaggaactcg gttttgcgtt gcttcagcga actgttcgct ggggtctgga
     2701 taaaattcgc cggtgtcagc cgccggccgg ttcaaccggt caggcggcac aggtgccggc
     2761 cgcccctccg caggaggatc ggtgacccac cacgaggaac cagcccttcc cgccccggga
     2821 atcgcttcga aagccagctt tgtctttatt cgctggtacc agcgctggct gtctccctat
     2881 atcggcgtac actgccgatt cgaaccgacc tgctcgcgct acaccttcgc ggccatcgaa
     2941 aagtatgggt tcatccgcgg ctggctgatg ggcatctatc gcattttccg ctgcaatccc
     3001 ttcaaccctg gcggttatga tccagttcgc tgatctgccg tcgtcggagg ctcatgtgac
     3061 acactctgcc cccctctcgt tttctcggcg gcttgccgtg ctgcgagccc tgaccgtcgg
     3121 tctgtggctg ctcgcctggt tgatcccgac acccttgtcg gcacaggcga ctgggacact
     3181 cgccccgatc gtcgctcccg ccgcaaccca ggcggtaacg accgaacttc gtgatcgcaa
     3241 cgcagacggc aaagccgacg tggccgtgga aaccccacgc ctgcggctgc tcatttccgg
     3301 tgccaccggc aatctcgaag tgttttatct gcgcggtatc aacttcgaag aaaacctgta
     3361 tcctccggtg atcgccgggc acgggtattc gttcgcgagc ctcaccatgc gtccgtttgc
     3421 cctgcagatc aatggctctg atgagccaga cggaacctgg acattcgaca ctccgaccgt
     3481 gggcacgcaa agcatcgtgg tcacggcgaa acggtccgaa cccgtttccg ggctcgagct
     3541 caccaaaacc tatcgcttca cgctcaatgg ttaccagttc ggatatgacg tgaccgtaac
     3601 gaattctacc ggcaaggaca tgatcgtcgg aaccgacgcc ctcggtggct tgaacctcat
     3661 gtatgggccc ggattgttca tcgatccctt caatccgtcc tcctatctcg ggctgcacga
     3721 tggctctcac atccagtatg acaacgtcgc gaagtttcgc gaagccgccg ccaaagaacg
     3781 ctttcaggga gtcggtctga agacgaccta tttctgcatg ctgatggatg ccggcattcc
     3841 ggtgaacctc atcgctcagg gcttcgaggt caaacccacc gatacgaaac agacccgcaa
     3901 ccgcactttc gaagggcagg tcgtcggagt atcgattccg ccgtttacgc tcaaggcggg
     3961 cgaatcgcgt cacgtgccgt tcacgctgta ttacggtccc aaactgctcg acgagctggc
     4021 gctgatcaat cgtggcaatg tcaccgacta cggatttctg agcacgatgc tgctgcgcat
     4081 tctgcagttc tttcacggcc tgttccccaa cttcggtctg gccatcattt ttctgactct
     4141 cgccgtgcgc gccatgctgc atccgctgac cgtcaagcag accaagtcga tggcccagat
     4201 gcagaagatc cagcccatgc tccaggatct caaggaccgt tatcgcgaca acccgcagaa
     4261 gttcaacgaa gaagttctca agctctacca gaaacacaat gtcaaccctc tgggcggctg
     4321 tcttcctctc ctgctgcagc ttcccattct cattgccttg tacaatacca tcaatattgc
     4381 cgtcgaactg cgcaaagtgc cgttcctctg gctgaccgac ctttcgaagg ccgacccgta
     4441 catcattctg cccctggcga tcacggccct gatgtatgcc cagcaagggc agaccacaga
     4501 cccgcagcag cagcagatga tggccttcat gccgatgttc atgttcatca tcacctggac
     4561 ccttccctcg ggtttgctgc tatactggtt caccagcagc gtcatcggag tggttcagca
     4621 gctccaggcc aataaaatgg tggccaaaat caaggaggag taggcagtca tgaaacgcgt
     4681 cattgaaatc accgccaaat cacacgaaga ggctcgcgca ctggcccaaa aagaagtgcg
     4741 gcccggcgaa acgatcaccc agtccgacat tttacaggaa ccgactcgtg gcatcttcgg
     4801 catcgtcggc aaccccgatg tccgcattcg cttcaccatc gacgagattc ccgcgccgcc
     4861 gccggtagct ccgaaggtcg tgacccccgc cactccttcc ccccgtcccg aaccagaagc
     4921 cgatttcggc gacgcggagg aagcgacgga tctcgacgaa gacctggtcg atgaagcaga
     4981 agacatctat gtcgacgagg agatcccggc cgacgccgag gctcatggcg gacgcaaacc
     5041 tcgcgtctac agcggaccaa ccggaaccaa ccacccggat tacgagcagc tcatccgtct
     5101 ggtgcatcag gtggccgcac tcgtgggcag cggcgaaatc accgtctcgg aacgcatcga
     5161 cggtgacgcc tggatcatcg aaccgaacgg cgacaatctg acccccttca tcgggaaaca
     5221 tggtcgaacc cttgatgcga tccaatatct cgtcaatatc gtggccaaca agggtcgtga
     5281 agacaaggtc aaaatcgtgc tcgacgctca gggataccgc gattcccggc atcgccgcct
     5341 gatctcgctg gctcatcgca tgtgccgcaa ggttgccgac ggcggacggg ccgtcgaact
     5401 ggaaccgatg tcgaccgtcg atcggcgcac gattcacctg gcactgaagg accgtacgga
     5461 tattcaaacc tattcgcgcg gcatcgaacc aatgcggcgg gtggtcatcg ctccgcgaaa
     5521 gggcgctcag gccggtggtg ggcgccgcgg cgggcagcga cctcgccggt atggcgggcc
     5581 aggccgcttc ggcggtcgcg accaacaggg agagcatcgc acccagggcg agcagaacgg
     5641 accacgcccg gtaccgatgt tcatgggcga ggatggtgac aacgactaga tcaaccatcg
     5701 ccgcgacggc caccccggtc ggtcgagcgg cgatcggcat gatcagaatc tcgggccccg
     5761 aaacgttcgc gattctgaaa caggtattcc gatacaacgg caaacgggct tttcctggcc
     5821 cgtttgccgt ttttcccggt catcttcagg atcccggtga tgggagcgct attgatcagg
     5881 tgctggtcac cacgttctgt tcgcccgcct catacacggg tgaagacatg gccgagatca
     5941 ctctgcatgg caacccggtc ctgctgtcga cggcgttgtc ggctctcttt caagccgggg
     6001 cacaccccgc cgaacccggg gaattcattc gccgcgcttt tctgaacggc aagcttgatc
     6061 tgctcgaggt cgaaggggtt tcgcagcttc tcagcgccgt cacgcgcaca caggcgcaga
     6121 tcgctctgca ccagctcgac gggttgccat cccagcgcat tcgtaccatt cgagaaacgc
     6181 tgctcgagcg actgactcat ctcgaagcca ctctgaattt tccggaagaa gccatcgatg
     6241 atctggacca gcaacagctg gccgccccac tgcgacaggc aacggctgaa ctcgagcgtt
     6301 ttctcgcggc cgcccgacag ggcagtcttc tggcagccgg cctgcgactg gttctcatcg
     6361 gcaagcccaa caccggaaaa tcgagtctgc tcaatcggct gatcggtcgt gagcgggcca
     6421 tcgtcaccga gattcccggc acgacccgtg acaccctgga ggagattttc tctcttgggg
     6481 gcttaccggt gcgtctgatc gataccgccg gccttcgcaa ctcggacgat cgtctcgagc
     6541 agctcggcat cgaacgtacc cgcgaagccg tggccggggc tttttttctc attgtcctgt
     6601 tcgatcggtc agaacccttt actgatgaag atcatttcac acttgagctg gtacgcaatt
     6661 ccaacaaacc tttcgtgatg gccttatcga agggcgatct gcccgatcgc ttctcgcatg
     6721 cgcccaccga ggtgacccag gcactcgtcc tttccacgag aaccggccag ggtattcagg
     6781 agttgacgga tctggttgca cgacgcctgc gcgaactcga acccggctca ctcgacgagc
     6841 tgatgtttct gggcgcgcag caattagcct cactggcaaa agctcttggc atgctgcatt
     6901 cgcttcttca gaacctggac catctctacc aggatctcat cgccgtcgaa ctcgaaggca
     6961 tcatacgtga ac
//
LOCUS       DAORHD010000063         6872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10883_length_6872_cov_3.141411, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000063 DAORHD010000000
VERSION     DAORHD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10883_length_6872_cov_3.141411"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2292
                     /locus_tag="PKO06_02385"
     CDS             82..2292
                     /locus_tag="PKO06_02385"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNV68517.1"
                     /translation="MKQGRELTQVIGRDGQRKAFDPEKIRFAIERAGKATGEFGALEA
                     AKLADQVIKILSHRHHKEIPQVEEIQDVVEHVLIVNNFFATNRSYIVYREQHKNMREG
                     RRTFVDVISSVNEYLGQQDWRVNANANQGYSLGGMILNVSGKVVANYWLSHVYTPEIG
                     EAHRNGDLHIHDLDMLSGYCAGWSLRTLLQEGFNGVPGKVEAGPPKHFSSALGQMVNF
                     LGTLQNEWAGAQAFSSFDTYLAPFVRRDNLTYHDVFQGIQEFVYNLNVPSRWGTQTPF
                     TNLTFDWICPEDLRQQKPLIGGQHQDFTYGDLQKEMDMINKAYIEVMTKGDHHGRAFT
                     FPIPTYNITKDFNWDHPNCEHLFEMTAKYGLPYFQNFVNSDLEPKMVRSMCCRLQLDL
                     RELLKRGNGLFGSAEQTGSIGVVTVNLARLGYQFAGDLNGLKSRIDELLTLSRNSLEV
                     KRKVIQRYIDDGLYPYTRRYLGTLRNHFSTIGINGMNECIRNFTRDEFDITTPKGVEF
                     AQNLLNHIREKMVQFQSETGHLYNLEASPAEGATYRFAREDKKRFPEIIQAGPKNAPY
                     YTNSTQLPVDFRGDIFEIMEHQTQLQTLYTGGTVLHLFVGERIPTAEACKKLVRKCLT
                     NFRMPYITVTPTFSICPTHGYTAGEHRFCPTCDSQLIKKFRAERDEAIAAGREAPVVP
                     EHASQIPSDLRQTCEVWSRVMGYYRPVSQFNAGKKSEYADRNQLQPTVLERHLA"
     gene            2328..3053
                     /locus_tag="PKO06_02390"
     CDS             2328..3053
                     /locus_tag="PKO06_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNV68518.1"
                     /translation="MLSQLNIGGMVPFTTIDFPGRLATVLFLRGCNLRCRYCHNPELL
                     DRSQRGTERWTRVESFLRQRQGFLEGVVLSGGEPTLEPRLPELASKIRDLGFEIALHT
                     NGACPDVIRHLIDERLVQFVALDVKGPFDQYQRICGVDQGMAAAATVRLLNESGLPHE
                     FRSTVHPDLLSDHDLLSLGDQMHELGARKLVLQRFREGKILDTSLNLVKRAWAHPTTI
                     RALEGRLGHIEIRGFDLPETIAA"
     gene            complement(3147..3683)
                     /locus_tag="PKO06_02395"
     CDS             complement(3147..3683)
                     /locus_tag="PKO06_02395"
                     /inference="COORDINATES: protein motif:HMM:NF022703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HNV68519.1"
                     /translation="MRKLFHYGLMIGVLIGATVAPAAAEEQSLNDSLKGLKSDMQDLK
                     QNLQGLKQGLKGTDSGTTTSGADNGTAITIPGVAVEIPGVTSGESQMYNDSHKEYTIE
                     CKGENLQINGSHCTFTLTGTCGQLQINGAYNTVKAEAVAQIHVNGAHNTVTYVKGTET
                     EQPTISNLGVENTIEVAK"
     gene            complement(3791..6766)
                     /locus_tag="PKO06_02400"
     CDS             complement(3791..6766)
                     /locus_tag="PKO06_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV68520.1"
                     /translation="MAIDPITTPDRLAGFLNDPNNRIRANVIIASVNKHGFDALVWLR
                     DLAAFGDPAYRRSALYVIDKLKHPSFIEVLEILICDDDLEVRHMAHKAIHEYADAGVK
                     KAKNLIEMADRMMAKTNMGEQDFHSAMVQMRAPTAQKKAVKKTSKSATEELGEQLLGS
                     DGLKAAGQSIKSLQEQAKVAREKMAARLSQTTASIVSGDSRERAVTIGASVVAVLLLL
                     AHAANAFSHFLSGSPQHRLALIAAVLSWLAGVPLLARRSAVPAALLPLVLFFVPWVAA
                     TLDIQDADLFGGTRVKKAASTPESSTSTASGTISTGSKRPPVRPATATRPVHTTTPPD
                     RHATATVVTNVPAAARAKILAPSKGDLLIGAFKVRAQISGKPKSAEILLDEKVIKTYA
                     ETHEGQITFDMTTETHDVGGHLLRVRVTDQAGRRAEEQVFVKFMTPISTVTIKAPASG
                     SVLWKDDHLLVDVTGNEYDQVEFALNDVVICTFPFDQAGLYNFPIPIATLTEGTHRFE
                     ARARMTDGRIATSAVQFKALNPKPTIAFESPKQGQQVYGSVDVIFLPDSGWKETAIRR
                     VTWSVDGLEKNTLASPPWQEIWEAGDMEPGPHVLKAVVENELERTAEASIRVEVIQPK
                     LAASIKGLRSNQILAEDLELTVEVVSDGPGTTIEEISVCVDNKLLKSLTKPPYSFVLK
                     VKEYDQGPHKLTAEVFRSDGQKFKTSPVTFSTNPSDRRTHLVAARSGTGALIGHEELA
                     KQRLELKEDGVAIASPSLQHADSGPQQLGLLMGIGASMKSEKRLSKAKEAAANLVDGL
                     KAHDRAFLLKFSDTAELAVAPTSDRTKLLQEIEYLAPQRGTAIFDAVARAVEESAKSP
                     GPWALVVFADRGDVGLDGRGPASKRSREEVFELLRRSNVQIHAVSLGNPSDTDFPAAD
                     EVLQALAKVSHGRYYNAPTESELPAAFQEVLKDLRSQIRVTFTSPSGAPDGKWHTIEI
                     QVRGNSDTKLRYKPGYLAR"
BASE COUNT         1338 a         2028 c         2028 g         1478 t
ORIGIN      
        1 ctttttttat caaaattctg tgtagtgtgt gcataacgcc ggctgatgcc ggtggatttc
       61 atatacaggg gcagagggaa catgaagcaa ggtcgggaac tgacgcaggt catcgggcgg
      121 gacgggcagc gcaaggcatt tgatccggaa aagattcgtt tcgcgatcga acgcgccggc
      181 aaggcaaccg gagaattcgg ggctctggaa gcggctaaac tggcggatca ggtcatcaag
      241 attctgtcgc accggcatca caaagaaatt ccgcaggtcg aagagattca ggacgtcgtc
      301 gaacatgttc tgatcgtcaa caacttcttc gccaccaacc gctcgtacat tgtgtatcgc
      361 gaacagcaca agaacatgcg cgaaggtcga cgcaccttcg tcgatgtcat cagttccgtg
      421 aacgaatacc tcggtcagca ggactggcgc gtaaatgcca acgccaacca ggggtactct
      481 ctgggcggca tgattctcaa cgtttcaggg aaagtggtcg ccaactactg gctgtcgcat
      541 gtgtacactc ccgagatcgg cgaagctcat cgcaacggcg atctgcacat tcatgacctg
      601 gacatgctct cgggctactg cgccggttgg tcgctgcgca ccctgctgca ggaaggcttc
      661 aacggcgttc ccggcaaggt cgaagccggt ccccccaagc atttttcgag cgcgctcggc
      721 cagatggtca actttctcgg cacgctgcag aacgaatggg ccggagccca ggcgttcagt
      781 tcgttcgaca cgtatcttgc tcccttcgtg cgccgtgaca atctgaccta ccatgacgtg
      841 tttcagggca ttcaggagtt cgtgtacaac ctcaacgttc cctcgcgctg gggtacccag
      901 acaccgttca ccaacctcac cttcgactgg atctgcccgg aagacctgcg gcaacagaag
      961 ccattgattg gcggccagca ccaggatttc acctacggcg atctgcagaa agaaatggac
     1021 atgatcaaca aggcctacat cgaggtcatg accaagggcg atcaccatgg ccgcgcgttc
     1081 acgttcccca ttccgaccta caacatcacc aaagatttca actgggatca cccgaactgc
     1141 gagcatctgt tcgagatgac cgccaaatac ggtttgccct actttcagaa cttcgtgaac
     1201 tcggatctcg aacccaagat ggtgcgctcg atgtgttgcc gcctgcaact cgacctgcgc
     1261 gaactgctca agcgcggtaa cggcctgttc ggctcggccg agcagaccgg ttcgatcggc
     1321 gtcgtgacgg tgaacctggc ccggctcggg taccagtttg ccggcgatct caacggcctc
     1381 aagagccgca ttgacgagtt gctgaccctc agccgcaaca gtctcgaagt gaagcgcaag
     1441 gtcattcagc gctacatcga tgatggtctc tatccgtata ctcgccgcta tctgggcacg
     1501 ctgcgcaatc acttctcgac catcggtatc aacggcatga acgagtgcat tcgcaacttc
     1561 actcgcgacg agtttgacat caccacaccc aagggcgtcg aattcgcgca gaatctgctg
     1621 aatcacattc gcgagaagat ggtgcagttc cagtctgaga ccggtcacct gtacaatctc
     1681 gaagcatcac cggccgaagg agcgacctac cgcttcgccc gcgaagacaa aaagcgcttc
     1741 cccgagatca ttcaggccgg tccgaagaat gccccgtatt acaccaactc cacgcagttg
     1801 ccggtcgact tccgcggcga catcttcgag atcatggaac accagactca gctgcagacg
     1861 ctctacaccg gtggtacggt gctgcatctg ttcgtcggcg agcgcattcc gaccgccgaa
     1921 gcgtgcaaga aactggtacg caaatgcctg accaacttcc gcatgccata catcacggtc
     1981 acgccgacct tctcgatctg cccgacgcac gggtacaccg ctggggaaca ccggttctgc
     2041 ccgacctgcg actcgcagct gatcaagaaa ttccgcgccg agcgagatga agccattgcc
     2101 gctggacgcg aagcgccggt cgtgcctgaa catgcgagcc agattccgag cgacctgcgt
     2161 cagacctgcg aagtgtggag ccgcgtcatg ggctactatc gcccggtttc gcagttcaac
     2221 gccggcaaga agagcgaata cgctgatcgc aaccagctgc agccgaccgt gctcgaacgc
     2281 cacctggcct gatcgacgaa gaccattcac cgacaaagga tctcactatg ctttcccaat
     2341 tgaacattgg gggaatggtt cccttcacca cgatcgactt tccgggtcgg ctggcgacgg
     2401 tcctgtttct gcgcggctgc aacctgcgct gccgatactg tcacaacccc gaactgctcg
     2461 atcgttcgca gcgaggcacg gaacgctgga cgcgggtcga aagctttctg cgtcagcggc
     2521 aggggtttct cgaaggcgtt gtgttgagcg gcggggagcc cactctggaa ccgcgattac
     2581 cggagctggc gtcgaagatt cgcgatctgg ggtttgagat tgccctgcat acgaatggcg
     2641 cttgtcctga tgtgatcagg catctcatcg atgagcgact ggtacagttc gtggcactcg
     2701 atgtcaaggg cccgttcgac cagtatcaac gaatctgcgg tgtcgatcag ggaatggccg
     2761 ccgccgcgac ggtgcgactg ctcaacgaga gcggcctgcc gcacgagttc cgctccacgg
     2821 ttcatcccga cctgctgtct gaccatgatc tgctcagtct cggagaccag atgcatgaac
     2881 tgggggcacg aaaactggtt ctgcagcgtt ttcgcgaagg gaagattctg gacacatcac
     2941 tgaatctggt caagcgcgcc tgggcgcacc cgaccacgat ccgcgcgctc gaaggtcgtc
     3001 ttggccacat cgagattcgt ggattcgatc tgccggaaac gatcgccgcc tgacgttcgt
     3061 caccccgatg aaaaagcggc cggctcatcg ccggccgctt ttctttctgc ttctgattct
     3121 cttctgaccg tttcccgtca gatcggttac ttggcaacct caatcgtgtt ctcgacgccg
     3181 aggttgctga tcgtcggttg ctcggtctcg gtgcctttca cataggtgac cgtattgtga
     3241 gcgccattga cgtgaatctg cgcgaccgcc tcggccttca cggtgttgta ggctccgttg
     3301 atctgcagct gcccgcaggt tcccgtcagg gtgaaggtgc agtggctgcc gttgatctgc
     3361 agattctcgc ccttgcactc gatggtgtat tccttgtggc tgtcgttgta catctgcgac
     3421 tctccgctgg tcacaccggg aatctcaacg gccacgccgg gaatcgtgat cgcggttccg
     3481 ttgtcggctc cgctggtcgt tgtgccgctg tcggttccct tcaggccctg cttgagcccc
     3541 tgcagattct gtttgagatc ctgcatgtcc gacttcagac ccttcaggga atcattgagc
     3601 gactgttcct cagcggcggc aggcgcaaca gtggctccga tcaatacacc gatcatcagt
     3661 ccataatgaa acagctttcg catggtgtcc tccacggcag tgatatcgaa ccagtccggc
     3721 tattatacac ggcctcggtg gttcaacaaa attagcgagc ccagcatgcg cggaatgcgc
     3781 agcgcctggg tcaacgggcc agatagccgg gcttgtagcg cagtttcgtg tccgagttgc
     3841 cgcggacctg tatctcgatc gtatgccact ttccgtctgg tgctcccgaa ggcgaagtga
     3901 acgtgaccct gatctgactt cgcagatcct tgagtacttc ctgaaatgcg gccggaagtt
     3961 cggactcggt cggggcgttg taataccgac catgtgaaac ttttgccaga gcctgcagca
     4021 cttcgtcggc agctggaaaa tcagtatcgg aggggttgcc gagactgacg gcgtgaatct
     4081 gcacgttact gcggcgaagc agttcgaaca cctcttcgcg tgaccgtttg ctcgcgggtc
     4141 cgcggccatc gagcccgacg tcgccacgat cggcgaacac gaccagggcc cacggacccg
     4201 gacttttggc gctctcttca acggcccggg cgaccgcatc gaaaatcgcg gttccacgct
     4261 ggggtgcgag atactcgatt tcctgtagaa gcttcgtgcg atcactggtt ggcgctaccg
     4321 ccagttccgc ggtatcggaa aacttcagca ggaaggcgcg gtcatgtgcc ttcaggccat
     4381 cgaccaggtt cgcggcggct tctttggcct tcgagaggcg tttttccgat ttcatcgaag
     4441 caccgattcc catcaggagt ccgagttgtt gtggaccaga atccgcatgc tgaagactgg
     4501 ggctggcaat cgccacgcca tcttctttca actcgaggcg ctgcttcgcc agttcctcgt
     4561 ggccgatgag cgcgcctgtg ccgctgcggg ccgcaaccag atgggttcgc cggtcagagg
     4621 ggttggtcga aaacgtcacg ggtgacgtct tgaacttctg tccgtctgat cgaaacactt
     4681 ctgctgtcag cttgtgcggc ccctgatcat actctttgac cttcagcacg aacgagtagg
     4741 ggggcttggt gaggctcttc aacagcttgt tgtcgacgca caccgaaatc tcctcgatgg
     4801 tcgtgcccgg gccatcgctg accacttcga cggtgagctc gaggtcttcg gccagaatct
     4861 ggtttgagcg cagacctttg atcgaagccg ccagctttgg ttgaatgacc tcgacgcgaa
     4921 tgctcgcttc cgcggtacgc tcgagttcgt tttccacgac ggctttgagg acgtgcggcc
     4981 ctggctccat gtcacccgcc tcccagattt cctgccaggg cggtgaggcc agggtgttct
     5041 tttcgaggcc atcgaccgac caggtgacgc ggcgtatggc ggtttccttc cagccgctgt
     5101 caggcaggaa gatcacatcg accgaaccgt acacctgctg cccctgtttc ggcgactcga
     5161 acgcgatcgt cggcttgggg ttgagggctt tgaactggac ggcactggtg gcgatgcggc
     5221 catcggtcat gcgggcccgc gcttcgaatc ggtgggttcc ttcggtcagc gtggcaatcg
     5281 gaatgggaaa gttgtagagg ccggcctgat caaagggaaa ggtgcagatc accacgtcgt
     5341 tcagggcgaa ctcgacttga tcgtactcgt tgccggtcac gtcgaccagc agatgatcat
     5401 ctttccagag cacggaacca ctggccggag ccttgatcgt cacggtagaa atcggggtca
     5461 tgaacttgac aaagacctgt tcttcggccc gacgaccggc ttgatcggtc acgcgaaccc
     5521 gcagcagatg cccaccgacg tcgtgcgtct cggtggtcat gtcgaaggtg atctgacctt
     5581 cgtgtgtctc ggcatacgtc ttgatcactt tctcgtcaag cagaatctcg gctgatttgg
     5641 gcttgccgct gatctgggcg cgaaccttga acgcgccaat cagcagatcg cccttgctcg
     5701 gcgcgagaat ctttgcccga gcggcagctg ggacgttcgt gaccaccgtg gctgtggcgt
     5761 gacgatcggg aggggtggtc gtgtgtaccg gtcgcgtggc tgtcgccggt cgaacgggag
     5821 ggcgttttga accggtggaa atggttccag atgccgtcga agtcgatgat tccggtgtgc
     5881 ttgctgcttt cttgacgcgt gtcccgccga acagatcggc gtcctgaata tccagggtcg
     5941 cggcgaccca cggaacgaag aagagcacga gggggagaag tgctgctgga accgcactgc
     6001 gcctggccag caacggcacc cctgccagcc aggacaggac ggcggcaatc agggcgagtc
     6061 gatgttgcgg tgaacctgac agaaaatgac tgaatgcgtt ggcggcatgg gccagcagca
     6121 ggagcacagc gacgacggaa gcaccgatgg tgactgctct ttctcgtgag tcgccggaca
     6181 cgatcgaggc ggtggtctga cttagtctgg cggccatctt ctcacgggcg accttggcct
     6241 gttcctgcaa gctcttgatc gattgtccgg ccgccttgag cccatcggag ccgagcagtt
     6301 gttctcccag ttcctcagtt gccgacttcg aggttttttt gaccgccttc ttctgcgccg
     6361 ttggggcgcg catctgaacc atggcggaat ggaaatcctg ctcgcccatg ttggtcttgg
     6421 ccatcatgcg atcggccatc tcgatcaggt tcttggcttt cttcaccccg gcatcggcat
     6481 actcgtgtat cgccttgtga gccatgtgcc gcacttcgag atcgtcgtca cagatgagaa
     6541 tctcgagcac ttcgatgaac gacggatgtt tcagtttatc gatgacatac agtgcgctgc
     6601 gtcggtaggc ggggtcaccg aacgccgcca gatcgcgaag ccagaccaga gcatcgaacc
     6661 cgtgcttgtt gaccgaggcg atgatgacgt tcgcgcgaat gcgattgttc gggtcgttca
     6721 gaaagccggc gagacggtcg ggcgtggtga tggggtcgat ggccatggtc gtttcgcgtt
     6781 caggcttcct ggcgggcggc ggagacggcg gacgcggggg ccgcgttgac gtccggccgc
     6841 gcgacgccga tgtcgatcac cgtcggcacc gg
//
LOCUS       DAORHD010000064         6848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_10943_length_6848_cov_3.729133, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000064 DAORHD010000000
VERSION     DAORHD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6848
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10943_length_6848_cov_3.729133"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKO06_02405"
     CDS             <1..239
                     /locus_tag="PKO06_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020606227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNV68521.1"
                     /translation="GFEKVPGIAEQILAALQQAGGFLPLTDGTAPEEIQKHFGISKKT
                     FKKAIGTLFRERRIVIEEQGIRLVPGKEERTGKS"
     gene            complement(243..1310)
                     /locus_tag="PKO06_02410"
     CDS             complement(243..1310)
                     /locus_tag="PKO06_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68522.1"
                     /translation="MYQILVSEPTLKRVDAYLQLLRLKNVAGKRLRDALEGFALETLD
                     GESFLERLVATKVPQIFAESAVRGDGSDWNPTELGLLGDIVIAVTVQVFDNGRHTQPE
                     VHPHPFPATLLFVPGALLRNDHGQTPADWDEVTREKQIVPRKFRCLYERRLLPALLFI
                     QATAERKGVKALVTIPGLGCGMFAGPFRGRLGSEFEKVLVGILEDHHTRLTQIRGLYY
                     DPYDECDNRRRVFGQTIFLTRPLRQGNDTRPQLCMPASYNDSVDDFSHCDLYSIVAWD
                     HVSWPGNDFYGGARATDDGVKAAATDVMTAITGVKGVYETRRTMYVPPAPYRTWGNVV
                     AELGLGLRVKGRVQVFPIPTP"
     gene            complement(1533..2429)
                     /locus_tag="PKO06_02415"
     CDS             complement(1533..2429)
                     /locus_tag="PKO06_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV68523.1"
                     /translation="MRRWFRDLLFLMATFTAIVTGAFAQNSTCTLRMAFTSDVFGNVP
                     LKDAEIAVQYWVELLAKDMGNTEYGWSRVFPDASSLFTAAGEQTFDILAMPPVEYLQQ
                     QERELYEPVLASTLMGNCMTRFLLLVHRDSTITELRQLRGKTLILQSYGCGRAHEMWL
                     DLQLKTAGEPRVREFFRRLTVEKEASKCALPVFFRQADACLMLNGSFDVLSEMNPQLK
                     KQLVTLKQSEEFLGGFVLFPKQMPPARKQQLRDICLRMSTYPRGRQVFTLFRIESLIS
                     FPPGALESLRSLLLRHMAEFKP"
     gene            complement(2437..4053)
                     /locus_tag="PKO06_02420"
     CDS             complement(2437..4053)
                     /locus_tag="PKO06_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013607826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV68524.1"
                     /translation="MINLNNVRVTFGKRTLFKNASFLIRPADKIGLVGPNGAGKTTVF
                     RLITGEEHPSEGDVQVQSGIVVGYFSQSVGEMSGRTALQEVLAGAGKVYEIGKKLHEL
                     EHRMADGTLDAAGMDRYGKLQEEFLHLDGYEKETHAQTILNGLGIGPDRWEQPVETFS
                     GGWKMRIALARILLLNPDVLLIDEPTNHLDVESIVWLEAWLKEFKGALVMTSHDREFM
                     TRVCFRTVEVAGETITTYSGDYDFYLRERTIRREQLIASQKRQQAMLAKEEEFIARFA
                     ARASHAAQVQSRVKTIEKIERIVVPPDPKVIKFEFGTTKRSGDIVVDLQGLGKTYTLP
                     DGRPLPVFSGATGKVIRQNKIALTGVNGAGKSTLLKIISGLIEPTEGVCNLGASVQLG
                     YFSQYSCDVLKPDRTVFEEVQERLPMATIGFIKNLLGAFQFSGDDSDKKVSILSGGEK
                     TRVMLAILLTQPVNFLVLDEPTNHLDIASREVLLEALQDFQGTVIIVSHDRYFLRHLV
                     NRVFEIDHGCLRTYEGDYDYYLSKTAAQAH"
     gene            complement(4097..4258)
                     /locus_tag="PKO06_02425"
     CDS             complement(4097..4258)
                     /locus_tag="PKO06_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68525.1"
                     /translation="MTGKTGDRVVIGGIYKCKDHPENTKTFLKGAVFLPCGRAEGHGT
                     TWILVRKTQ"
     gene            4685..6040
                     /locus_tag="PKO06_02430"
     CDS             4685..6040
                     /locus_tag="PKO06_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNV68526.1"
                     /translation="MKLRIVHVLTFCILLVFSSSAVFSASSLPDKTVLEAVAHGNMAF
                     AVDLYQRLCQKEGDLFFSPFSISSALAMTRAGACASTASQMDAVLYFTGLPEQQALHE
                     TFAAFLKTLNTSQEGLSLAISNSLWGHKNYAFLPAFLSQLEQAYGARLERVDFHAAAE
                     ARKQINDWVAKQTKDLIKDLLPDGIITPLTRLILVNAIYFKGMWEAAFDKDDTRDLPF
                     YLRNGKAVRTPIMNRTGSYSYAEATDLQILEIPYKGGKVAMTIFLPAKPDGLAALENG
                     LTADLLQQHVQSLSRVEKVNVYLPRFKSTGSFSLSETLKALGMTEAFSLEKADFSGMT
                     EKRELAISEVVHKAFVDVSEEGTEAAAATAVVMQLRCAAPMEKKIPVFRADRPFLYVI
                     RDLTHGNLLFMGRFARPAGEEIDPSTLKEQSSPAVIQTGSDLVPAPAPPAPTPDDTNR
                     E"
     gene            6176..>6848
                     /locus_tag="PKO06_02435"
     CDS             6176..>6848
                     /locus_tag="PKO06_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68527.1"
                     /translation="MGEIILFLVIGILITPVLIIIHIYRRLSDLRSRIEENFANDQAE
                     KHVIRRALDRLRQLEETVQQLSAARGAGTTPTLVEPTPSCSITANETAAEKIVTDTAM
                     VSDQRPSSVQGSLPPEPGERVRATATASAGAPGTGEPSERVAHESFLPPIPTETKAVP
                     RAVPASGQPLPETPRTSVRQPEWEAVIGGNLLNKLGAFILVIGLGLFLYYTLDSFGPA
                     AKLLIG"
BASE COUNT         1489 a         1933 c         1926 g         1500 t
ORIGIN      
        1 caggcttcga aaaagtgccg ggaatcgccg agcagattct ggcggcgtta cagcaggcgg
       61 gaggttttct gcctctcacg gatggtaccg ctcccgaaga gattcagaag cacttcggga
      121 tcagcaaaaa gaccttcaaa aaggcgattg gaaccctgtt tcgcgaacga cgcattgtca
      181 tcgaagagca gggaatccgc ctggttccgg ggaaggaaga gagaaccgga aaaagctgag
      241 ccctacgggg tggggatcgg gaagacctgt actctgcctt tgacgcgaag cccaagaccg
      301 agttcggcaa ccacgttgcc ccacgttcga tagggtgcgg gaggaacata cattgttcgg
      361 cgtgtttcgt aaacaccttt caccccggtt attgccgtca tcacgtccgt ggccgcggct
      421 ttcacgccgt catccgtagc gcgcgcccct ccatagaaat cgtttccggg ccaggatacg
      481 tgatcccagg cgacgatact gtacaagtcg cagtgcgaga agtcatcgac ggaatcgtta
      541 taagaagcgg gcatgcagag ttgcggtcgg gtgtcgttcc cctgacgcag tggccgcgtc
      601 aggaagatcg tctgtccaaa cactcgccgt cgattgtcgc actcatcgta tgggtcgtag
      661 tacaaaccac gaatctgtgt taaccgcgta tgatgatctt caagaatacc gacaagtacc
      721 ttttcaaact cagagccaag tcgaccgcga aatggcccgg caaacatgcc acagccaagt
      781 ccaggtatgg tcaccagcgc cttgacgcct ttacgttcgg cggtggcttg tatgaacagg
      841 agtgccggca ggagtcgtct ttcatacaga caccggaact tccgggggac gatctgtttc
      901 tctcgtgtga cttcgtccca gtcggccgga gtctgaccgt ggtcattacg cagcagagcg
      961 ccgggaacaa acaggagagt tgcgggaaac gggtgtgggt gaacctcggg ttgcgtatgc
     1021 cgaccattgt cgaacacctg cacggtaacg gcgatgacga tatcgccgag aaggccgagt
     1081 tcggtcgggt tccaatcgct gccgtcgcct ctgacagcac tttcggcaaa gatctgcggc
     1141 accttggtgg ccacaagtcg ctccagaaaa gattccccgt cgagcgtctc gagagcgaaa
     1201 ccttcgagag cgtcgcgtaa acgcttgccg gccacattct tcagacgcag caactgcaga
     1261 taggcatcaa ctctcttcag ggttggctct gagacaagaa tttgatacat cgtttccgtg
     1321 ctccttgcgt gcggtgtggt cagggcgttc gaagtgaagc caaaggacag gagaccgaaa
     1381 agcaggaccc gaagccagct caccgggcgc tgcttcatgt cagtcgcaga cccacgtttc
     1441 cgtcgttgag caccaggcgt ctgttcccag ccgacccgtc agcatgtgcc atacgataga
     1501 gacgatgaaa gccacacctg aaggcgcgag agtcatggct tgaattctgc catgtgcctg
     1561 agcaatagcg aacgcaacga ttccagagct ccagggggaa agctgatcag gctttcgatg
     1621 cgaaacaacg tgaaaacctg acgtccacgg ggatacgtgg acatgcgcag gcagatatcg
     1681 cgcagttgct gcttgcgtgc gggaggcatc tgtttcggaa acaacacgaa accacccaga
     1741 aactcctctg attgcttcag cgtcaccagc tgctttttca attgcggatt catctcactc
     1801 aggacatcga aggagccgtt caacatcaga caggcatcag cctgcctgaa gaatacagga
     1861 agcgcgcatt tcgaagcttc cttttctacg gtcaaccggc gaaagaactc gcggacgcgt
     1921 ggttcaccag cggttttcaa ctgcaggtcc agccacatct catgagccct gccgcaaccg
     1981 tatgactgca gaatcagcgt tttgccccgc agctgtcgca gctcggtgat ggtgctgtcg
     2041 cgatgcacca gcaggagaaa ccgggtcatg cagtttccca tcaacgtcga agctaacacg
     2101 ggctcataca gttcgcgttc ctgttgctgc agatactcga ccggcggcat ggccagaata
     2161 tcgaaggttt gctctcctgc tgcggtaaac agggaagagg catcgggaaa aacccggctc
     2221 cagccgtact ccgtatttcc catgtccttg gcgagcagtt caacccagta ttgaacggca
     2281 atttcggcat ctttcagggg gacattgccg aacacgtcgc tcgtgaatgc catgcgtaga
     2341 gtacaagtcg aattctgagc aaaagctcct gtgacaattg cggtgaatgt tgccatcagg
     2401 aacagaagat cgcgaaacca tcgtctcatg ccggcgtcag tgcgcctggg cggcggtctt
     2461 gctcaggtag tagtcgtaat cgccctcgta cgttctgagg cagccgtggt cgatttcgaa
     2521 aacacggttc accaggtgtc gcagaaagta ccggtcgtga cttacgatga tcacggtccc
     2581 ctgaaaatcc tgcagggcct cgagtagaac ttcgcggctg gcgatatcga gatggttggt
     2641 cggctcgtca agcaccagga agttgaccgg ttgtgtcagc agaatcgcca gcatgactct
     2701 ggttttctct ccgcccgaca gaatcgagac ctttttgtcc gagtcgtcgc ccgagaactg
     2761 aaaggcgccg agcaggtttt tgataaatcc gatggtagcc atgggcaggc gttcctgtac
     2821 ttcctcgaac acggtacgat cgggtttgag cacatcgcag gaatactggc tgaaataccc
     2881 gagttgaacg ctggcgccga ggttgcagac gccctcggtc ggctcgatca agccgctgat
     2941 gatcttgagc agcgtcgact tgccggcccc attgaccccg gtgagcgcaa tcttgttctg
     3001 gcggatgacc ttgccggtcg ctcccgaaaa aaccggtaag gggcggccat ccggcagagt
     3061 gtaggttttt cccagaccct gcagatcgac cacgatatcg ccgctgcgtt tggttgtacc
     3121 gaactcgaac ttgatgactt tcgggtcggg cggcaccacg atgcgctcga tcttctcgat
     3181 cgtcttgacc cgagactgca cctgcgccgc atgcgatgca cgagccgcga accgggcgat
     3241 gaactcttct tctttcgcga gcatagcctg ctgacgcttt tgtgatgcga tcaactgctc
     3301 gcgtcgaatg gtacgctcgc gcagatagaa gtcgtagtca ccactgtacg tggtgatggt
     3361 ttcgcccgcc acttcgacgg ttcgaaaaca aacccgggtc atgaactcgc gatcgtgact
     3421 ggtcatgacc agcgcgccct tgaactcctt gagccaggct tcaagccaga cgatcgattc
     3481 gacgtcgagg tggttcgtcg gttcgtcgat cagtaataca tcgggattca gcagcagaat
     3541 gcgagccagt gcgatccgca tcttccagcc gcccgagaag gtttcgaccg gctgttccca
     3601 gcggtccggg ccgatgccca gaccgttcag aatagtctgc gcatgtgttt ccttctcgta
     3661 gccgtcgaga tgcaggaact cttcctgcag ttttccgtac cggtccattc cggcggcatc
     3721 cagcgtgcca tctgccatgc ggtgctcgag ttcgtgcagt ttcttgccga tttcgtagac
     3781 ctttccggct ccggccagga cttcctgcag ggcggtacgc cccgacattt cacccacgct
     3841 ttgcgaaaag tagccgacga cgatgccaga ctgaacctgg acatcaccct cgcttgggtg
     3901 ttcctctccg gtgatcaggc gaaagaccgt ggtcttgccg gcgccgttcg gcccgaccag
     3961 gccgatcttg tcagccgggc gaatcaggaa cgaggcattc ttaaaaagcg tgcgcttgcc
     4021 gaacgttacg cgcacattgt tcagattgat catggccggt cactcatggc agaatggacg
     4081 ccttattggc gcgttgtcac tgcgtttttc gcaccagaat ccaggtggtg ccatgccctt
     4141 cggcccgccc gcaggggaga aagaccgccc ctttcagaaa ggtcttggta ttctccggat
     4201 gatctttgca tttgtatatc ccaccgataa cgactcgatc tcccgttttg cctgtcatga
     4261 gagaactccc tggcgatgaa gatggttcga tcgtttcagg aaactctctg aacgaaagaa
     4321 ggcagaacct ttacctgacc gtgtctgcct aacggacaac cgggacgcaa cgacttctgc
     4381 gacaacgtaa gaacaaagac caatgctctg ccatgcatct cgttgcacca gtataccaca
     4441 aacgctcacc gctggctccg aattggcaga catgaccggg gcacacagga acttcctgct
     4501 ggcgcagaag tgtttgtctt actccaccca aggaacaccc ggacgagcct gaattcctcg
     4561 caagtggccc atcatgagag tgtgcagggg gtctggctgg cggatgactg gacaagcagg
     4621 tgctggtagc ggtaggtttt tgtccgaacc ccggttttta cttcttcatt tctggagatt
     4681 cctcatgaaa ctgcgaatcg tgcacgtctt gacgttctgt atcctgctgg tcttctcgtc
     4741 ttccgcagta ttttctgctt ccagtttgcc tgacaagact gttctcgaag cagttgccca
     4801 cggaaacatg gcatttgcgg tcgatctcta tcaacgcctg tgccagaaag agggcgactt
     4861 gtttttctct ccgttcagca tctcgtcagc actggcgatg acgcgtgcgg gagcgtgcgc
     4921 ttcgaccgcg tcgcagatgg atgctgtcct gtatttcacg ggtctgccag agcagcaggc
     4981 tcttcatgag acattcgccg cgtttctgaa aaccctgaac accagtcagg aaggtctttc
     5041 gttggccatt tcaaactcgt tgtgggggca taagaactat gcgtttctcc cggcatttct
     5101 gtcgcagctc gaacaggctt atggcgctcg tctcgaacgg gtcgactttc atgccgcagc
     5161 cgaagcgcga aagcagatca atgactgggt ggccaagcag acgaaagatc tgatcaagga
     5221 tctgctgccg gatggaatca tcactcctct tacgcgcctg attctggtca atgccatcta
     5281 cttcaaggga atgtgggaag ccgcttttga caaggacgat acccgcgacc tgccattcta
     5341 tcttcgcaac ggaaaagccg ttcgcacgcc gatcatgaat cgaacaggat cgtactctta
     5401 cgcagaagcc accgacctgc agattctcga gattccctac aagggaggga aggtcgccat
     5461 gacgatcttt ctgcccgcga agccggatgg tctcgccgct ctcgaaaatg gtctcacggc
     5521 tgatctgctg caacagcacg tgcagagtct atcacgtgtg gaaaaggtga acgtgtatct
     5581 gccgcgcttc aaaagtacgg gttcattttc tctctcagag actctgaaag ctcttgggat
     5641 gaccgaggca ttttctctcg agaaggctga cttctcgggt atgacggaaa aacgtgaact
     5701 ggcgatcagt gaggtggtcc acaaggcctt cgtcgatgtc agtgaagaag gtaccgaagc
     5761 ggcagccgcg accgccgtcg tgatgcagtt acgctgtgcc gcaccgatgg aaaagaagat
     5821 tccggtcttt cgcgctgatc gaccatttct gtatgtgatt cgagacctga cgcacgggaa
     5881 tctcctgttc atgggtcggt tcgcgcgacc tgccggtgaa gaaatcgatc cttcgaccct
     5941 gaaggaacag agctcgccag cggtcattca gaccgggagc gacctggtgc ctgcccctgc
     6001 accgccagca ccgacgcccg acgatacaaa cagggagtag tcttccaatc gtgctgtaaa
     6061 ggagaacccg ggcttgatgc tcgggttctc tctgtatcga gagggttttc accagcgatt
     6121 gccgttggtg tagaatgata cgtttgaatt ctgttttcgt cagcggagca cgagcatggg
     6181 tgaaataatc ctgtttctgg tcatcggaat cctgattacg ccggttctca tcatcattca
     6241 catctatcgc cgcctgagtg atctgcgcag tcgcatcgaa gagaactttg ccaatgatca
     6301 agcggaaaag catgtgattc gccgcgcgct cgaccggctg agacagctcg aagaaacggt
     6361 gcagcagctt tcggccgcac gaggcgccgg aaccaccccc acgctggttg agccgactcc
     6421 gagttgttcc atcacggcca atgagacggc tgccgaaaag atcgttactg acactgccat
     6481 ggtcagcgac cagaggccca gttcggttca ggggagtttg ccgcctgagc cgggcgagcg
     6541 ggtgagggcg acggcgactg cttcggctgg tgctcccgga accggcgagc cgtccgagcg
     6601 agtggctcac gagtctttcc tgcccccgat tccaacggag accaaagcag ttcccagagc
     6661 cgtgccagcc tctggccagc cgctccccga gacaccgaga acgtctgtca gacaaccgga
     6721 gtgggaagcc gtcatcggtg ggaaccttct caacaagctc ggagcgttta tcctggtgat
     6781 cgggctggga ctctttctgt attacacgct cgactccttc ggaccggcgg ccaaactgtt
     6841 gatcggtc
//
LOCUS       DAORHD010000065         6788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11078_length_6788_cov_3.572108, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000065 DAORHD010000000
VERSION     DAORHD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11078_length_6788_cov_3.572108"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="PKO06_02440"
     CDS             complement(<1..535)
                     /locus_tag="PKO06_02440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68528.1"
                     /translation="MTPERERSTRSIRLRFRNPRYDRIVRLNTESIARRMGFNEDQVF
                     DITLAVEEAYTNAIEHSRRSFDSLELEIIYHLFQDRLVVSIQDSGCGFEQGEPGIDQP
                     LPVADQIRGRGLGLILNLSDHAEILSEPGLGTMIRITKFLAGAPGSRVGDVPRIPTCP
                     GSPEARSSLDDNKQPDEV"
     gene            1034..1678
                     /locus_tag="PKO06_02445"
     CDS             1034..1678
                     /locus_tag="PKO06_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68529.1"
                     /translation="MSEIRKKAVKTSYQRVATGTNQTLSESFALEQITQWEEQAVDLA
                     SRAQTASNREQMLSAHQALLQKLEATRELAALLGFASDTRRLASFTVDVEKLSPSQKQ
                     RFSELKTILGLTGIPATPVPLIVRPARVERQPALPTNLQTVPTTQLPAPATFSRQAAS
                     PQVATEPIELTSPVKPAKIPDRFYRPGRIPLLGDTSEEENEDKTETASESTESQ"
     gene            1821..2834
                     /locus_tag="PKO06_02450"
     CDS             1821..2834
                     /locus_tag="PKO06_02450"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNV68530.1"
                     /translation="MTKRHAFGIVGATGAVGQKFLRILEEQQIEPSHIRLFASEKSQG
                     RKVTFRGQEYTIECVEGADFKGLDVVFFSAGTEVSKTFCPQVVKAGAVVIDNSNAFRM
                     EPQVPLVVPEANAHALKHHQGLIANPNCSTIQMIVAIAPIHQVNPIKRIVVSTYQSVS
                     GTGLEAIETLKQQATAKLEGEALPEGVYPHPIGFNLFPHIDVFLESGLCREEEKMVRE
                     TQKILESPIPVCPTTVRVPVFYCHSESVNLELSQAMSPEEARALLSKSPSIVLMDEPD
                     RNVYPTPLLCQDRDEVFVGRIRRDPSVTNGLNLWVVADNLRRGAATNGVLIYRSLVER
                     GQL"
     gene            2967..3980
                     /locus_tag="PKO06_02455"
     CDS             2967..3980
                     /locus_tag="PKO06_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68531.1"
                     /translation="MLVVLLLFFIHATAWADYRRPNESDPFVRSILWGTHTARALVQL
                     HDEPFHVVPGTTIDRTWYVSEVRRQSVLFRNRKTGTFVDAPLVTREKPRFNRLTSFWS
                     HEISLWDAVELVSMGYKYSVVMHYETGGSVKPQCHADSLERMLQKFMPPHVRFAIVGP
                     VLVVLPNRIYQENWRDILKRVKEINPDATAQRYSGLMQSGTVHSRGDDIQFVLRQISL
                     GSGVMIFFPSSLHFPVYAAFKDMPFHHILTMIAAVNQCVLIDRVGGIEFQPMSQTRAI
                     FPGPQLPDMLTVGPWEPQPGSGPHPPAPVPWAPADPGAWRRLPPPAIPASEAAVISPA
                     DSF"
     gene            3994..5235
                     /locus_tag="PKO06_02460"
     CDS             3994..5235
                     /locus_tag="PKO06_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV68532.1"
                     /translation="MTLTNPPPIGKAATVRLGASDPQTDLSVVPCRDYAETTIKQALL
                     QLLKPFGGLGNLVHSGDRVLLKPNLLSARGPDAAATTHPLLVEFLASACRQLGAQVFI
                     GDSPPLTLRRIEDFWEQTGMAQAAQRSGATLVCFEREPRRELTVAGPHGPVRIHVTEE
                     VFKANVTINLPKLKTHNLTVLTGAVKNHYGLLPGLQKAQWHRLLPQPDDFSRLLVSIC
                     EATPMTLHLMDGIMGMDGQGPAGGRPVPTGVLLAATNPAAVDLGVCSITGADPTTVPT
                     LHWYQRLQQRPYNREHLRFHGADPRHLRFSDFRLPPPHLVTFIPQALVRVARRLFWLR
                     PALKPGLCIKCGACLKICPAHAISMREQGIRFHRATCISCFCCMEVCPRDAIVMQGSS
                     VVSLLMAARRLKHRWRQRNGG"
     gene            5239..6156
                     /locus_tag="PKO06_02465"
     CDS             5239..6156
                     /locus_tag="PKO06_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV68533.1"
                     /translation="MNFWLRDLMQHAQGRLIDRLARWSAHSAYRQRWRAAQLLTGISF
                     DLLRLRRGHVETALRERLGISASAAVQQARDVYQHFFLNALEMASIPYLQREELLARI
                     EARGLDHLRAALDRGRGCIILSGHYGLWEMVPPWMSYHGFPMTVVVRRQNNPHVDEWM
                     EFMRQAHGPKTTDSGFTLRQILRALKNGESLGLMSDQDAGTKGLFVNFLGKEASTVVG
                     PAQIALKTGSPIVPLCLHPQHPHPHQLEICAPIFPDRYADDLEGQIRITQAYTDILAE
                     WVRRRPEQWFWLHRRWKTRPTPQQDESPA"
     gene            6153..>6788
                     /locus_tag="PKO06_02470"
     CDS             6153..>6788
                     /locus_tag="PKO06_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNV68534.1"
                     /translation="MNFSLSLYQSVLRAIVTALELVPPRRIEREEVELRKGHYAPETE
                     VPTATVWIHGASLGEVITLRPLLKALAQAVGRRHLLGTTMTMDGLKRLRADDLVEFTS
                     LMPVELPEFLDPFIARLRPSLLLIAETEIWPLLLSTARRHGLPYGIINGRINPKTVKV
                     LRLFASLFRTGLHDLKFVLCQSQEYADRYASLGIPRSRIQVLGSFKHDLEPV"
BASE COUNT         1420 a         2098 c         1825 g         1445 t
ORIGIN      
        1 gcacttcatc aggttgcttg ttgtcatcaa gagagcttct tgcctctggg gaacctggac
       61 acgtgggaat ccgcggaaca tccccgacgc gcgaacccgg cgcacccgcc aggaacttgg
      121 ttatgcgaat catggtgcca agccctggtt cagacaagat ctccgcatga tctgacagat
      181 tcaggatcaa tccgagcccg cgaccgcgaa tctgatccgc gacgggaagg ggttgatcaa
      241 taccgggctc gccctgctca aagccacaac ctgaatcctg aatcgacacg acgagtcgat
      301 cctgaaacag gtggtaaata atctcgagtt caagagaatc gaagctgcga cggctgtgct
      361 cgatcgcatt ggtatacgct tcttcgaccg ccagcgtgat atcgaagact tgatcttcat
      421 tgaagcccat tcggcgggca atcgactccg tgttcaatcg tacgatccga tcgtaacgag
      481 ggttacggaa ccgcaagcga atcgacctgg ttgatctctc tcgttccggt gtcatgataa
      541 tctcatccgc ctatgagtat agcatctttt tccacgcctc gaaatgctcc cccccagcat
      601 tcatggacga tgtgcgtctc tcaacggtcc ccatcctgat ttccgaaatc gatggcttgc
      661 cccccttgtc cgtatcgtga aggagaggta tcttcgacag agcatggaaa tcgatcacca
      721 cacaccctgc tgcgggcaat cacggcgaaa acggcactgt cttccacgac tgctcctgtc
      781 gctttttttt ctctggccac tgggggcatc ggcacaggat ctgccctcgc ttccagccct
      841 ggatcaactt cctgcctgga tgaacctcaa gcccggacag gatgcaagtg cggtcatacc
      901 gatttcgcag cccgtatggc gagagcctca gggaccaatc gatcctgatc cgtccgctgc
      961 tctcaagacg ccacaggcac ctcggagcag tggtcgggtt cagttccagt tcactccacc
     1021 cgcgatgccc gacttgtctg aaattcgcaa gaaagccgtg aaaacatcgt atcaacgagt
     1081 ggctaccggt acaaatcaga cactcagcga gagttttgcc ctcgagcaga tcacccagtg
     1141 ggaagaacag gccgtcgacc ttgcctcacg agcgcagaca gcctcaaatc gcgagcagat
     1201 gttgtccgcg catcaggcac ttctccagaa gctcgaagca acccgggaat tggcagctct
     1261 acttggattc gccagcgata cccgacgatt agcatctttc acagttgacg tagagaaact
     1321 gtcaccctct cagaaacaac gtttttccga attgaagacc atccttggtc tgaccggcat
     1381 tcccgcaaca cctgtgccct tgatagtgcg accggcgcgc gttgagcgcc aaccggcact
     1441 tccgacaaac ctgcaaacgg tgccgacaac gcaactccct gccccggcca cattttcacg
     1501 tcaagcggct tccccacagg ttgcaaccga accgattgag cttacctctc cagtgaagcc
     1561 ggccaagatt ccagatcgat tctatcggcc cgggcgtatt ccgttgctcg gagacacatc
     1621 ggaagaagag aacgaggaca agacggaaac cgcgtcagaa agcacggaga gccagtaacc
     1681 acagggagcg cacccgacgg tgaccgcgcc ggttcttgag cgacagctgg agcgcatcaa
     1741 catcagcttc aggcgggctt tacatcacgc ccgctttgcg gtacaagtgg cgcagagcaa
     1801 tgttcacgaa aggatggggt atgaccaagc ggcacgcatt tggaattgtc ggcgctaccg
     1861 gagccgtcgg acaaaagttt ctgcgcattc tcgaagagca acagattgag ccttctcaca
     1921 ttcgtctctt cgcctcagaa aaaagtcagg ggcggaaggt cacgtttcgc ggccaggaat
     1981 acacgatcga gtgcgttgag ggagccgact tcaaggggct cgacgtcgtg ttcttttcgg
     2041 ccgggaccga ggtcagcaaa acgttctgcc ctcaggtcgt caaagccggc gcagtcgtca
     2101 tcgacaacag caacgccttc cgcatggaac ctcaggttcc tctcgtggtg cccgaggcaa
     2161 atgcgcatgc cctgaagcat caccagggat taatcgccaa tccgaactgt tcgacgattc
     2221 agatgatcgt cgccatcgca ccgattcatc aggtgaaccc catcaagcgc attgtcgtca
     2281 gtacctatca gtctgtctca ggaactggac tggaagcgat tgagacgctg aagcaacagg
     2341 ctacggccaa gctagagggg gaagccctgc ctgagggcgt gtatccgcat ccgatcgggt
     2401 tcaatctgtt tccgcatatc gacgtctttc tcgagagcgg tctgtgccga gaagaagaaa
     2461 agatggtccg cgaaacgcag aagatcctcg aaagcccgat tccggtgtgt cctacgactg
     2521 tccgcgttcc ggtgttttac tgccacagcg agtcggttaa tctcgaactc agtcaggcca
     2581 tgtcacccga agaggctcgt gcactgcttt ccaaaagccc gagtatcgtc ctcatggacg
     2641 aaccggaccg caacgtctac ccaactccgc ttctctgcca ggatcgtgac gaagtcttcg
     2701 tcggccgcat tcgacgagat ccgagcgtca cgaacggctt gaacctgtgg gtggttgccg
     2761 acaatctgcg gcgcggcgcg gcgaccaacg gcgtactgat ctatcggagc ctggtcgaac
     2821 gcggacaact atagagtaca agtaacgatt tctcaaattg agctttacaa aagagataca
     2881 agctgagcac ttttcgacac acacgatctc aggatagtga ccttgagcgc ggcagaacag
     2941 aggctctgct gagtttccga ctgccggtgc tcgtcgtatt gctgctcttc ttcattcacg
     3001 ccacggcctg ggcagactat cggcggccca acgaatctga cccattcgtg cgctcgattc
     3061 tgtggggcac gcatacggcc agggccctgg tgcaacttca tgacgagcct tttcacgtcg
     3121 taccgggcac cacaattgat cgcacctggt atgtttccga ggtgcggcgc caatcagtcc
     3181 tgttccggaa ccgcaagacg ggaacctttg tcgacgcccc tctggtaact cgagagaaac
     3241 ccagattcaa tcgactgacc tccttctggt cgcatgaaat cagtttgtgg gatgcggttg
     3301 agctggtctc catgggatac aagtacagcg tcgtcatgca ttatgaaacc gggggttcgg
     3361 tgaaaccaca gtgtcatgcc gattctctcg aacgcatgct gcagaaattc atgccccccc
     3421 acgtgcgatt cgcgattgtc gggccggttc tcgtagttct cccgaaccgt atttatcagg
     3481 aaaactggcg cgatatcctc aagcgcgtca aagaaatcaa ccctgatgcc accgcgcagc
     3541 gctacagcgg cctgatgcaa tccggaaccg tgcattctcg cggcgacgac attcagttcg
     3601 ttctgcgcca gatttcactc ggttccgggg tcatgatctt cttcccgtcg tccctgcatt
     3661 ttccggtcta tgccgcgttc aaagacatgc cgtttcacca cattctgacg atgatagctg
     3721 ccgtgaatca atgtgtactg atcgatcggg tggggggcat cgagtttcag ccgatgtccc
     3781 agacacgcgc gatctttccg ggtccgcagc tccctgacat gctgaccgtc gggccctggg
     3841 aaccacagcc aggcagcggt cctcatcctc ccgccccggt gccgtgggca ccggctgacc
     3901 caggtgcctg gcgtcgcttg ccaccgcctg cgattcctgc atctgaggca gccgtcatct
     3961 cgcctgccga cagtttctag tgcgactcat ctcatgacgc tcacgaaccc accccccatc
     4021 ggcaaggccg caacagtcag gctcggcgcc tctgatccac aaacggatct ttccgtggtc
     4081 ccctgccgtg attatgcgga aacgacgatc aagcaggctc tcttgcagct tctcaagccg
     4141 tttggcggtc ttggcaacct ggtacattca ggtgaccgcg tcttgttgaa acccaatctc
     4201 ctgtcagccc gggggcccga tgcagcggcc acgacgcacc cgcttctggt cgaatttctg
     4261 gcttctgcct gtcggcaact cggtgcccag gttttcatcg gagacagccc gcccctgacc
     4321 ctgcggcgca ttgaagactt ctgggagcag acgggaatgg cacaggcagc gcagcgttct
     4381 ggggcaaccc tggtctgctt cgaacgcgag ccacgacggg aacttaccgt cgccgggcct
     4441 cacggaccag tgcggattca cgtcaccgaa gaggttttca aggccaacgt caccatcaat
     4501 ctgcccaaac tgaaaacaca taatctcacg gttctgaccg gtgccgtgaa aaaccactat
     4561 gggctgctcc ccgggttgca gaaagcgcag tggcatcgtc tgttgccgca acccgacgac
     4621 ttcagccgcc tgctggtctc gatctgcgaa gcgacaccta tgaccctgca cctgatggac
     4681 gggatcatgg gcatggatgg gcagggcccg gcgggtggtc gcccggtgcc gaccggcgtg
     4741 cttctggccg caaccaaccc ggcagcggtc gatctcggtg tgtgttccat cacgggcgct
     4801 gatccgacga cagtaccgac tctccattgg tatcagcggc ttcaacaaag gccctacaac
     4861 agagagcatt tgcgttttca cggtgccgac ccacgacacc ttcgtttctc cgatttccgc
     4921 ctgcccccgc cacacctggt aaccttcatt cctcaagccc tggtgcgcgt tgcccgccgg
     4981 ctgttctggc tgcgtccagc tttgaaaccc gggctttgca tcaaatgcgg tgcctgcctc
     5041 aaaatctgtc cggcacacgc catttccatg cgggaacagg gtattcgttt tcatcgtgcc
     5101 acgtgcatct cctgtttctg ctgtatggaa gtgtgtccac gcgatgccat tgtaatgcag
     5161 ggcagttccg tggtttcctt gctgatggcc gcgcgccggc tcaaacatcg ctggcgccaa
     5221 cgcaacggag gctgaggcat gaatttctgg ctccgcgacc tgatgcaaca cgcacaggga
     5281 cgcctgatcg accgactggc ccgctggtcg gctcactccg cgtatcgaca gcgttggcgc
     5341 gcggctcaac tgttaaccgg tatctctttc gatctcctgc gccttcgccg cggccacgtc
     5401 gaaaccgcgc tgagggaacg actcggcatc tccgcatccg cagcggttca gcaggcccgc
     5461 gacgtgtatc agcatttctt tctcaacgcg ctggaaatgg cctcgattcc gtatctacag
     5521 cgcgaggaat tgctcgcccg catcgaagcg cgcggtctcg atcatctgcg tgccgctctt
     5581 gatcgcggtc gtggctgtat cattctttcc gggcactatg ggctctggga gatggttcca
     5641 ccctggatga gctatcacgg gtttcccatg accgtggtgg tgcgacgtca gaacaatccc
     5701 cacgtcgacg agtggatgga attcatgcgc caggctcacg gcccaaaaac aaccgattcc
     5761 ggctttacct tgcggcagat tctgcgcgca ctcaagaacg gcgaaagcct cgggctcatg
     5821 agcgatcagg atgccgggac gaaagggctg ttcgtcaatt ttctgggtaa agaagcctca
     5881 accgtcgtcg gaccggcgca gatagcgttg aaaaccgggt ctcccatcgt tcccctgtgt
     5941 ctgcatccac agcatccaca tccacatcag ctcgagatct gcgcgccaat cttccccgac
     6001 cggtacgctg atgacctcga ggggcagatt cgaatcacgc aggcgtatac ggacattctg
     6061 gctgaatggg tgcgtcgacg tcccgagcag tggttctggc tgcatcgtcg ctggaaaacc
     6121 cgacccacgc cacaacagga tgaatcaccc gcatgaactt ttctctgtct ctctaccaaa
     6181 gcgttctccg ggcaatcgtg acggctctcg aactggtgcc tccccgtcgc atcgaacgcg
     6241 aagaggtcga actgcgcaag gggcactacg cccctgaaac cgaagtccca acggcaacgg
     6301 tctggattca cggcgcgtcg ctcggagagg tgatcacact gcgcccgctg ctcaaagcgc
     6361 tcgcgcaagc tgtcggacgc aggcacctgc tgggaacgac catgaccatg gatggcttga
     6421 aacgattgcg cgctgatgat ctcgttgagt tcacctcgct gatgccggtc gagctgcccg
     6481 agtttctcga tccgtttatc gcccgcctgc gaccgtccct gctgctcatc gcggaaaccg
     6541 agatctggcc gctgcttctg tcgaccgctc gacgacatgg cttgccctac ggcatcatca
     6601 atggccgcat caacccgaag acggtgaaag tgttacggct gtttgcttcg ctctttcgta
     6661 ccggtcttca cgatctcaag ttcgttctct gtcagagtca ggaatacgct gatcggtatg
     6721 ccagtctcgg cattccccgc agtcgcattc aggttcttgg ctctttcaag cacgatctcg
     6781 aaccggta
//
LOCUS       DAORHD010000066         6747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11176_length_6747_cov_4.040048, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000066 DAORHD010000000
VERSION     DAORHD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11176_length_6747_cov_4.040048"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1169
                     /locus_tag="PKO06_02475"
     CDS             318..1169
                     /locus_tag="PKO06_02475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV68535.1"
                     /translation="MNPRPRAEALPSFYPDAFFGARRNPREVLLEQSESMQQKWLLLR
                     DLRPGRILDIGCQKGEFLYFMQSRGWQGKGIEISPLSPNLFGQDIHYGSLAEAQLRPG
                     SFDVVTLWAVLEHLLEPQRMLRDILPLLAPHGRLIVLVTNIQSLPGRWMRHDDIPRHL
                     LLFSPQTLRSMLERCGWLVQRVHCNHVLFGGWHRGLFTYLLKWLAGESFADIVAQNRT
                     PERWLEFTTHLRGKPSRLVEWVETWDKRLTPWFDTLMDRLGFGFTLIATATPRSPQRL
                     ICHGRIR"
     gene            1169..3136
                     /gene="asnB"
                     /locus_tag="PKO06_02480"
     CDS             1169..3136
                     /gene="asnB"
                     /locus_tag="PKO06_02480"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV68536.1"
                     /translation="MCGICGWVYTDPARPAPVEVIRRMLGRLRHRGPASGGVWATKNA
                     ALGHARLPIIDLSPHAAQPIISEDGNSILVCNGEIYNFEDLRSQLETRGHRFRSRSDS
                     EVILHLWEEKGENCVHDLIGMFAFAIWDIRRELLFLARDRFGKKPLVYAQYPDRFVFG
                     SEIKALQCDPGFRPAVSERGLYLYLAYQSVPAPYSAFDRVEKLPPAHCLTVRQGRGEL
                     REYWRLKHAPRFADTAEPPPEIVTELQERLQQSVKCRIRADVPVGAMLSGGIDSSLVV
                     SIIARQLGQQVSTFTVGFPIAEYDERPWASEVAKRLSTNHFELLAGPEIIDLLPTLVW
                     YYNEPFADSSALPTWLVCRLAREHVTVALSGDGGDECFAGYPRYQNIGEHALSEGFPG
                     IWERWHRRFRDWRGFVTGRSWTADLRRLADLHQDRLLYYYRLAHFHEGYQTRLLTPEF
                     RRRLGDLVAVDHLLERFRRSRAPSFLDTLTEIDLGLYLPDTLMTKTDVASMAHSLEVR
                     SPFLDHTLVEFCARFPAHWKLKDGCQGKWILRRAAADLLPESILHRKKMGFGIPMDHW
                     FRKEWRDVAHDALLSGRLIARGYFEPDYLRSLLERHQQGESWQYLIWNLMMFEHWHRR
                     FIDGDGPPMPDPELTITEVRNDRRESGDPPR"
     gene            complement(3081..4502)
                     /locus_tag="PKO06_02485"
     CDS             complement(3081..4502)
                     /locus_tag="PKO06_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV68537.1"
                     /translation="MTRLHPLPPAWSPRQLLSRAQEWLDAWKKGTWAEHAHPVPLEGP
                     FFREIDRCWGLQMPELAALADTPEFNQRSSLILMEDGRELGPPHSLHEIIRKHGSGSY
                     SHWGRILMFSTSDNSDPNTNGRVYSYRFRPPAEHPLHHVAHWNLRNRFLAALYWLIYV
                     VCLVPPFRRAFRWVWNVLPELMSRLVAPGMISSSLLSTTGLPVNQRERDCSPQALRQD
                     VEHAIRVARQYLSRLPGGGAGLRGKTIMEIGPGINVGSSLILACHGATVFVVDRFLCT
                     WVEEYHARFYATLLQAMPAAFPGVPTTVIETFLSHRQAPEIRAFAHPLENLRTVPYHS
                     IDIFLSNAVLEHCADTWAACREMTRVARPGALAFHQVDFRDHRDMERPLEFLLLDSES
                     FAGEFETRNGECGNRVRARELEQMFREHQWEVTSFEPNFFAEQSYLRDFIPRFQSANP
                     SLAQRFNEEDLRILCGHFSLRLS"
     gene            complement(4499..5659)
                     /locus_tag="PKO06_02490"
     CDS             complement(4499..5659)
                     /locus_tag="PKO06_02490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV68538.1"
                     /translation="MRNGTGKPRVMMIIHQFRPWRPGGAEVQAERLAERLAQRGHAVH
                     VLTQQRTPDALPVENWRGFRITRVPFALGYRPFSGWSQFAFLVRYRRSYDILHVHQAF
                     GHAIVAVLAAQLLRKPCLIKLGCAGTYGDLHQFSRLPLSAFGLPILRQADVIVAISRE
                     IEQELQTWQFAPDRILCLPNGIDTTQFRRSAGWGRPDEKRGPWRLCLVGRRHPQKGID
                     VAIRAMALLRHEPGFPPCELHLYGVDYPEYDFRRLSAECGVEDRVIFHDFRDEPRAIL
                     DTHDLFILPSRGEGLSNALLEAMSMELPIIATRVSGTTDLLVDGWNGRLIPPESPEAL
                     RAAICDVLRRPEQAGEWGRRARRTVESLYSLDYIAAQYSALYDRLLARLPQS"
     gene            complement(5667..>6747)
                     /locus_tag="PKO06_02495"
     CDS             complement(5667..>6747)
                     /locus_tag="PKO06_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68539.1"
                     /translation="LCGPALGHRVFATERAGDQSSPIVLQLSNVVGVKVVPWPEDMKP
                     DMRLPVVPSSVFLSWPLNSWLLSSGDAATVSTPLPALIGFGMNSVTGKPIWFYLPVTG
                     EAIQSHAVLTRPLIRAPRQEMNPYVRLIDADSLPAPLSSYLKSDKPERQRLCELCDWY
                     SRRIAFGTGTHEMFHHADSALARSRVREQLFQSSAPLELYCYQECVLLADLLGDFGCS
                     VRLLHLDYEPFPAMMHDRRIQHWVAEIRLGEGDRWLFVDPSLGVFGLTSADGRRVVDW
                     LRVGGLWSYRLPLVTLKPRWVQYTWEIASRRLHDAAVHGPSLACVAIAEYLRAFDYGA
                     FLACRLRGVREYAPHGWSQIVEVTR"
BASE COUNT         1354 a         2059 c         2019 g         1315 t
ORIGIN      
        1 cgggctcccg gccctcagtg cgaactgatt tacgacgcct gttctttcga cgtgctgccc
       61 acgaagggca tcttcaccac ctcactggtc gacttgcagg caacgtatga agtcgaggtt
      121 cgtccaccgt gactcgatca cgcttcagtc gtcatttcac tgggactttc gatacttccg
      181 cgatgctgga aactgtcacc tgcccacttt gcggccaatc cgattgccga gaactgtttc
      241 gtcgacctga tcatacatac caggtctcag accacgcttt cccggtcgtt cgctgtcgtc
      301 actgttcgat ggtgtacgtg aatccccgcc cgcgcgccga agcactgccg tcattctatc
      361 ccgacgcgtt cttcggggcc aggcggaatc cgcgcgaggt gcttctggag caatcggaat
      421 cgatgcagca gaaatggctg ttactgcgcg atctgcgtcc gggtcgcatt ctggatatcg
      481 gctgccaaaa gggtgagttt ctgtatttca tgcagagtcg ggggtggcag ggaaagggca
      541 tcgagatttc cccgctttct cccaatctgt tcggacagga catccattac ggatcgctgg
      601 cggaagcgca attgcggccc ggctccttcg acgtcgtgac tctgtgggcc gtgctcgagc
      661 acctgctcga acctcagcgg atgttgcgtg acattctgcc gctgctcgcc ccgcatggtc
      721 ggttgattgt cctggtcacc aacattcaga gcctgcccgg gcgctggatg cgccacgacg
      781 atatcccgcg acatcttctg ctcttctcac cgcagaccct gcggtcgatg ctggagcgat
      841 gcggatggct ggttcagcga gttcactgca atcacgtgct gttcggcggc tggcatcgcg
      901 gcctgttcac ctatctgctc aagtggctgg cgggagagtc tttcgcggac atcgtcgccc
      961 agaatcgaac ccctgagcgg tggctcgagt tcaccacgca tctgcgcggc aagccctcgc
     1021 gtctggtcga gtgggtggaa acctgggaca agaggctcac tccgtggttc gacacgctga
     1081 tggaccgcct cggattcggc ttcacgctga tcgccaccgc gactccccga tcgccacaac
     1141 gtctgatctg tcacgggaga attcgctgat gtgcggcatc tgcggctggg tctacacgga
     1201 tcccgcacga ccggcgccgg ttgaggtgat tcgtcgcatg ctgggtcgat tgcggcatcg
     1261 cggaccggcc tcgggagggg tctgggcgac gaaaaacgcg gctctgggtc atgcccgcct
     1321 gccgatcatc gatctgagcc cgcacgccgc acagcctatc atcagcgaag acggcaacag
     1381 cattctcgtc tgcaacggtg agatctacaa cttcgaggat ctgcgaagcc agctggaaac
     1441 acgcggtcat cgtttccgat cgcgttccga ttcagaagtg attctgcacc tgtgggaaga
     1501 gaaaggcgag aactgtgttc acgatctgat cggcatgttc gccttcgcca tctgggacat
     1561 acgccgcgag ttgctgtttc tcgcccgcga ccgtttcggc aagaagcctc tggtctacgc
     1621 gcagtatcct gaccgcttcg tcttcggatc ggagatcaag gctcttcagt gtgatccggg
     1681 gtttcgcccg gcggtcagcg agcgcggcct ttacctgtat ctggcctacc agtcggtgcc
     1741 ggcgccgtac tcggcgttcg atcgcgtcga gaaactgccg cccgcccatt gtctgaccgt
     1801 gcgtcaggga cggggtgagc tgcgcgaata ctggcgtctg aaacatgcgc ctcggttcgc
     1861 cgacaccgcc gagcccccgc ccgaaatcgt caccgaactt caggagcggc tacagcagtc
     1921 ggtcaaatgc cggattcgcg ccgacgtgcc tgtcggagcc atgctgtcgg ggggcatcga
     1981 ctcatcgctg gtcgtgtcga tcatcgccag gcagctcggc cagcaggtgt cgacattcac
     2041 cgtcggtttt ccgatcgctg agtatgacga gcgcccctgg gccagcgaag tcgcgaaacg
     2101 cttgagtacg aatcactttg aacttctggc aggacctgaa atcatcgatc ttctgcccac
     2161 gctggtctgg tactataacg aaccgttcgc cgactcgtcg gcgttgccga cctggctggt
     2221 ctgtcgcctg gcacgcgagc atgtgacggt agcgctttcg ggcgatgggg gcgacgagtg
     2281 tttcgccggt tacccgcgtt accagaatat cggcgaacac gcgctcagtg aagggtttcc
     2341 gggcatctgg gaacgctggc atcgtcggtt tcgcgactgg cgcggttttg tcaccggacg
     2401 gtcctggact gccgatctgc ggcgtctggc cgacctgcat caggatcgtc tgctctacta
     2461 ctaccgtctg gctcactttc acgagggata ccagacccgc ctgctgactc ccgagttccg
     2521 ccgccgtctg ggcgatctgg tcgctgtcga tcacctgctc gaacgcttcc gccgcagtcg
     2581 agcgccgtcc tttctcgaca cgctcaccga gatcgatctg gggctgtatc tgcccgacac
     2641 tctgatgacc aagacggacg tcgcctcgat ggctcattcg ctcgaggtgc gctctccgtt
     2701 tctcgaccat acgctggtcg agttttgcgc ccggtttccg gcgcactgga agctgaagga
     2761 cgggtgtcag ggcaaatgga ttctgcgtcg cgccgccgcc gaccttctgc cggaatccat
     2821 tctgcatcga aagaaaatgg gattcggcat tccgatggat cactggttca gaaaggagtg
     2881 gcgggacgtc gctcatgacg cccttctttc cggtcgactg atcgcacgcg gctacttcga
     2941 acccgactat ctcagatctc ttctcgaacg gcaccagcag ggcgaaagct ggcagtatct
     3001 gatctggaat ctcatgatgt tcgaacactg gcaccgccga ttcatcgacg gagacggccc
     3061 gccgatgccc gacccggagc tcacgataac cgaagtgaga aatgaccgca gagaatccgg
     3121 agatcctcct cgttgaatcg ttgggccagc gacgggttcg ctgattgaaa gcgaggaatg
     3181 aaatctcgca ggtaggactg ttccgcgaag aagttcggct cgaaactcgt gacttcccat
     3241 tgatgttcgc gaaacatctg ttccaactcc cgggctctga cccgattgcc gcattcgccg
     3301 ttccgcgtct cgaactcacc ggcgaacgat tcggaatcga gcagaagaaa ttcgagcggc
     3361 cgttccatgt cacgatgatc gcggaagtcg acctgatgga acgcgagcgc gcccggtctg
     3421 gccacccggg tcatctcgcg acaggcggcc caggtgtcgg cacaatgttc gagcacggca
     3481 ttcgagagaa agatgtcgat cgaatggtac ggaacggttc gcaggttctc gagcgggtgc
     3541 gcgaacgctc gtatctccgg cgcctgtcgg tgggacaaaa aggtctcgat caccgtggtc
     3601 ggcaccccgg gaaaggcagc cggcatcgcc tgcagcaagg tcgcgtagaa gcgggcatgg
     3661 tattcttcga cccacgtgca gagaaatcgg tcgaccacga acaccgtggc cccgtgacag
     3721 gcgagaatca gggaactgcc gacattgata cccggcccga tctccatgat cgtctttcct
     3781 cgaaggcccg cgccgccgcc cgggagacga ctgagatact ggcgggccac tcgaatcgcg
     3841 tgctcgacgt cctgtctcag ggcctgcggc gagcagtcgc gttcacgttg attgaccggc
     3901 aatcccgttg ttgacaacaa gcttgaagaa atcattccag gcgcgaccag cctggacatt
     3961 agctcgggca gaacattcca gacccagcga aaggcgcggc ggaagggcgg caccagacag
     4021 accacgtaga tcagccagta gagcgcggca agaaagcgat tccgcagatt ccaatgcgcg
     4081 acgtggtgca atggatgttc ggcgggcgga cgaaaacgat aggaatagac acgcccattc
     4141 gtgttagggt ccgagttgtc actggtcgag aacatgagaa tgcggcccca atgagagtac
     4201 gagccgctgc catgcttgcg aatgatctca tgaagactgt gcggtggtcc aagttccctt
     4261 ccatcttcca tgaggatcag cgacgatcgc tgattgaact cgggtgtatc cgccagtgcc
     4321 gccagctcag gcatctgcag gccccagcac cgatctatct cgcgaaagaa cgggccctcg
     4381 agcggcaccg gatgggcatg ttccgcccag gtgcctttct tccaggcatc gagccattcc
     4441 tgcgcgcgcg acaacaactg tcgaggcgac caggcaggcg gtagcggatg caagcgggtc
     4501 atgactgggg aaggcgcgcc aacaaacgat cgtagagagc ggaatactgg gcggcaatgt
     4561 agtcgagtga atagagactt tccaccgtac gtcgcgcgcg ccgcccccat tccccggcct
     4621 gctcgggtcg gcgcaggaca tcgcagatcg cggcccgcag ggcctcgggg gattccggcg
     4681 gtatcagtcg gccgttccag ccatcaacga gcaggtcggt ggtaccactg acccgggtgg
     4741 cgatgatcgg cagttccatg gacatcgcct cgaggagagc attggaaaga ccctcgccac
     4801 ggctgggcag tatgaataga tcatgggtat cgagaatggc tcgcggctca tctcgaaaat
     4861 catggaagat cacccggtct tcgacgccgc attccgccga gagacgacgg aaatcatact
     4921 cgggatagtc gactccgtag agatgcagtt cgcatggcgg gaatcccggc tcgtgccgca
     4981 gaagcgccat cgcgcgaata gcgacatcga tgcctttctg cggatgtcgt cgaccgacca
     5041 gacacagccg ccacggccca cgcttctcat cgggcctccc ccaaccggcg gagcgtcgaa
     5101 actgcgtcgt gtctatgccg ttcggcaggc acagaatgcg gtcgggcgcg aactgccacg
     5161 tctggagctc ctgctcgatc tcacggctga tggcgacgat gacgtccgcc tgtcgaagta
     5221 tcggcaggcc gaacgcggaa agcggcagtc gcgaaaactg gtgcagatct ccgtaggttc
     5281 cggcacaccc cagtttgatc aggcagggtt ttctcagcag ttgcgcggcc agaacggcga
     5341 cgatggcatg accgaacgcc tgatggacgt gaagaatgtc ataggagcgc cggtatcgca
     5401 cgaggaaggc gaactggctc cagccgctga agggtcgata cccgagcgcg aacggcaccc
     5461 gcgtgatgcg aaagcctcgc cagttctcga ccggcagagc atcgggggtt ctctgctgcg
     5521 tgagcacgtg aacggcatgt ccgcgctgtg ccagtctttc cgccagacgc tcggcctgca
     5581 cttcggcacc gcccggacgc cagggtcgaa actgatggat gatcatcatc accctgggct
     5641 tgcctgtgcc gtttctcacg tgtttcctac ctcgtcactt ccacgatctg cgaccagccg
     5701 tgcggggcat attctctcac acccctcagg cgacaggcaa gaaaagcccc ataatcgaaa
     5761 gcgcgcagat actcggcgat tgcgacgcaa gccaggctcg gaccgtgcac cgccgcgtcg
     5821 tgcagacgac gactggcgat ttcccacgtg tattgcaccc agcgaggctt gagggtcacc
     5881 agaggcagtc gataggacca gaggccaccg acgcgcagcc agtcgaccac tcgacggcca
     5941 tccgctgacg tcaaaccgaa cactccaaga gaaggatcga caaacaacca acgatcccct
     6001 tcgccgagcc ggatctccgc gacccagtgc tgaatgcgac gatcgtgcat catcgccgga
     6061 aagggttcgt agtccaaatg caagagtcgg acggaacatc cgaaatcgcc cagaaggtcg
     6121 gccagaagca cacactcctg atagcagtaa agttcgagcg gagcactcga ttgaaacagt
     6181 tgttcgcgaa ctcgtgaacg ggctaacgcg ctgtcggcat ggtgaaacat ctcatgcgtt
     6241 ccggttccga atgcgattcg acgactgtac cagtcacaca attcgcatag tcgttgtcgt
     6301 tcaggtttat ctgacttcag atacgaactc agcggcgcag gcaagctgtc agcatcgatc
     6361 agccgcacat atggattcat ttcctgtcgc ggagcacgga tcaaagggcg cgtcagcaca
     6421 gcatgtgact ggatagcctc gccggtgacg ggcaggtaga accagatggg cttccccgtg
     6481 accgaattca ttccaaaccc gatcagtgca ggtaacgggg tggaaaccgt cgccgcgtct
     6541 ccggaggaca gcagccatga attcaagggc caggacagga agacggacga agggacaacc
     6601 ggaagccgca tatccggctt catatcttca ggccatggca ccaccttcac gccgacgacg
     6661 ttcgacagct gtaacacgat gggtgacgat tgatctccag cccgttccgt cgcgaacacc
     6721 cggtggccca gtgccgggcc gcacaac
//
LOCUS       DAORHD010000067         6717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11261_length_6717_cov_3.780847, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000067 DAORHD010000000
VERSION     DAORHD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11261_length_6717_cov_3.780847"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKO06_02500"
     CDS             complement(<1..264)
                     /locus_tag="PKO06_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNV68540.1"
                     /translation="MIDKNQLDAFRLEAPFQLRGDQPRALGQLSTWLNDGRSFLTLLG
                     VTGSGKTFTMASLIQRVQRPVLVITHNKTLAAQLYSEFRDFFPH"
     gene            364..1182
                     /locus_tag="PKO06_02505"
     CDS             364..1182
                     /locus_tag="PKO06_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNV68541.1"
                     /translation="MMKIYIIADMEGIAGVVSPDQISGNSWEYDLARKQFNAEVCAVC
                     SGAIEGGAEEIYVNDFHGNGRNLDPVLLPQQVMLVRGEFRPCSGFDHLDNTFAGLILL
                     GAHARSGSRDAVIPHTYNPDIRFELYGQPVGEFDLLALLAGEMKVPTLLISGDAKTIE
                     QAHTNLPATATVITKFGLTGQSAVCLHPKAICEMLREETKRAMQAASTLEPPMISPPV
                     QLLVKLTNVSTAERLDWIPGLKRVDELTYEFMGETMVEVMKLVYGVTTLVSAKF"
     gene            1193..1921
                     /locus_tag="PKO06_02510"
     CDS             1193..1921
                     /locus_tag="PKO06_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNV68542.1"
                     /translation="MPYVFLVVADHTRFVPEYLAPPPFRALLEIGGVPAIEKMLQALG
                     RVSRLSMQVLTTDEDPVKRVLAPRRQIPIHGARANPVDLIREAVQDLPDEDIVVFMPG
                     DLPFVSKTEVEELIRLAGQEPGVVCPVVKTITLPPGLKKKSLVFQEGELCPGYALAAH
                     RRVLTPDLFRRIDQVLQGPTQLLKNIGGRFLLKMLFSKPHLGEAEELASSHLGVPVRL
                     VIMPSAGFARNLSSEEDLTAAGVL"
     gene            complement(2039..3538)
                     /locus_tag="PKO06_02515"
     CDS             complement(2039..3538)
                     /locus_tag="PKO06_02515"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV68543.1"
                     /translation="MENWHDLRDLATQLFAEVAPRGVKDLDRLFEKWKSGFPRKQEVN
                     FEQALPPDMRQQVLADIGIVEPPEVSSGSSHYLSYLTILNRLMERNIEELLQMSAEDV
                     RALIVREGRRLQLSDLEIGELTTVLPQWIQKISNKVIFEFSPINIFEMTSPAKESGGS
                     SSARFKGIIGQSAKMQSIFSCLERISASNLTVLIQGESGTGKELVAHAIHMNSPRSQE
                     NFIPVNCGALPDSIIESELFGHERGAFTGAVGQKIGYFEIADKGTIFLDEISETSLNT
                     QVKLLRVLQEKKIRRVGGTRPIDVDIRVIAATNRDLLTLVREGSFRHDLYYRINEMTV
                     TLPPLRERQGDLPLLIEHFLEKFAKENGRKTVPRLDPATRRLLFSYSWPGNIRELENV
                     LKRTVVLADAVIQPSHLPPNLLEKPARSGSARSTSVSTGSLKDQLMSAEREIIQQSLE
                     NNRGNVSLTARVLKVSRRTLQRKMKALGIEGGDQENESENRPPAPDRAP"
     gene            complement(3547..4512)
                     /locus_tag="PKO06_02520"
     CDS             complement(3547..4512)
                     /locus_tag="PKO06_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68544.1"
                     /translation="MHVSRLCLTLLTLLAMLLASGNAWAIRNICNDCGQVAEPAEVTC
                     SKCDEPLNICLACDTMNEVSADFCSKCGEDLAYMRVLATIDPDTRDELRLGQSDRARL
                     ERSLARLNHFLEKDPQNAETYLYRRAKVYQQMGFFSNEANAWADFLRQFPDTPKMTRI
                     QGFQSEALRKWGFLFYQQGQLKSAQEKFLLATKANPMNTEAWLWAGRCAMEAKHTTEA
                     AEYYLSGLKSDPGNKTAIHFLRQLKKTPPKDLLVAPKPAGAVDKKPAVTPKADQKPAP
                     VVQDSPAAVVPDTLATPSPSTEPTGNTGRVNPEASSQKPPIKQGN"
     gene            complement(4585..5424)
                     /locus_tag="PKO06_02525"
     CDS             complement(4585..5424)
                     /locus_tag="PKO06_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68545.1"
                     /translation="MSEIIELAQFRAFSFEEDPEALVDMQRCREHLEGSWFDREETVR
                     MRQKIVCRALGSSWVVTLARTIVGYADLIGMPSGEGLVPHWRLHPDYRDPRLGRKLLN
                     GLQEAAAKRSYKGLAFFCDIPEVQEDLERLGIPRSRSFFWAKASDLEGGEPLEITESR
                     EELPELMNRNYLPFLGTPLPPKLVFAQAHMASSYGVFSYQRPRLGSVLFHDRQFVLCF
                     DGREWYIFRTEKHPEDGDAIRPLLETLGSWHDGRILLSAKALEKAGLEAMSAEPVWDC
                     FVG"
     gene            complement(5491..>6717)
                     /locus_tag="PKO06_02530"
     CDS             complement(5491..>6717)
                     /locus_tag="PKO06_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNV68546.1"
                     /translation="WLVMLTFFCSPASAESIRSETEALEAFFTGSVPPVELFTTEFLQ
                     AVPPAKIKEIASMYHSEIGSWKNSAGASGSYTLCFDRGEAPCRIHLDQTGRIDGFWMG
                     APKHHHDSVASISTTFRALPGTTAITLTRNQDTILLDHNGDKPLAVGSAFKLYILKAL
                     RQGIREGKWSASQTVALNPDWKSLPSGILQDWPIGTPITIETLANLMISRSDNTATDH
                     LLFLVGRETVEKLDPHQNEPFLSTMEMFRLKYLDPIWRTRYLASTEPAHRREVLTAMA
                     TLPVLLTEIKEDPIEVQNIEWFMTTRNLCGILRELREDPSLAINPGLVDKDKWYRVAF
                     KGGSEPGVLNYTICLQKAPASPTYCLSATINQPTQPLETKTFDVLILRLIALIEKGEI
                     DKMPASVASDRENSAK"
BASE COUNT         1339 a         1817 c         2081 g         1480 t
ORIGIN      
        1 gtgcggaaaa aagtcgcgaa actcgctgta gagctgcgcg gccagggttt tgttgtgcgt
       61 gatcaccagc accggccgct gcacacgctg aatcagactg gccatggtga acgtcttgcc
      121 gcttcccgtg actccgagaa gggtcagaaa ggagcgccca tcattgagcc aggtggaaag
      181 ctgaccaaga gctcgtggct ggtcgccgcg tagctggaag ggggcttcga gacggaacgc
      241 gtcgagttga tttttatcga tcatgggcgt agattatacc tgaatctgac cgctccgccc
      301 agaggagaag ggccgtcctt ccgctgctga acgctgcaga aaaactcaca ccaaaacagg
      361 ttgatgatga agatttacat tattgctgac atggaaggga tcgccggtgt cgtttccccc
      421 gaccagattt cgggtaactc ctgggaatac gatctggcgc gaaaacagtt caatgccgag
      481 gtgtgcgccg tttgttcggg agcgatcgag ggcggcgcgg aggaaatcta cgtcaacgac
      541 tttcacggga atggccgcaa tctcgacccg gtgctgttgc cacagcaggt catgctggtc
      601 cgtggcgagt ttcgcccctg ctcgggcttt gatcatctcg ataacacctt tgccggactg
      661 attctcctgg gggcccatgc gcgcagtggg tcgcgcgatg cggtcattcc tcacacctac
      721 aacccggaca tccgtttcga actctacggc caaccggtcg gggaattcga tctactggct
      781 ttgctggccg gtgagatgaa ggtgccgacc ctgctgattt cgggcgacgc gaaaacgatc
      841 gaacaggcgc ataccaatct gccggcgacg gcgacggtga tcaccaagtt cgggctgacc
      901 gggcagtcag cggtttgcct gcatcccaaa gccatctgcg aaatgttgcg cgaggaaacg
      961 aaacgtgcca tgcaggccgc ttcgaccctc gagccgccga tgatttctcc gccggtacaa
     1021 ctgctggtca agctgacgaa tgtgtcgacc gccgaacggc ttgactggat tccaggactg
     1081 aagcgggtcg atgaactgac ctacgagttc atgggggaaa ccatggtcga ggtcatgaaa
     1141 ttggtatatg gagtgacaac gctggtttca gcgaagttct aacccccttc tcatgcccta
     1201 tgtttttctg gtcgtcgccg accatacacg cttcgttcct gagtatctcg ctccaccccc
     1261 attccgggct ctcctcgaga tcgggggagt gccggcaatc gaaaaaatgt tgcaggcgct
     1321 cggtcgggtg tcgcgactgt cgatgcaggt tctgaccacg gatgaagacc cggtgaaacg
     1381 cgtgttggca ccgcgccgac agattccgat tcacggagcc cgggcgaatc cggtcgatct
     1441 gattcgtgaa gcggtacaag atcttcccga tgaagacatt gtcgtgttca tgcccggcga
     1501 cctgccgttc gtctcaaaga ccgaggtcga ggagttgatc agactggccg gccaggagcc
     1561 gggagttgtc tgccccgtgg tgaaaacgat cacgttgcct cccggactca agaagaagtc
     1621 gctggtgttt caggaagggg aattgtgccc gggatatgct ctcgcggctc atcggcgagt
     1681 tctgactccc gatctgtttc ggcgaattga ccaggttctt caggggccga cgcagctgct
     1741 gaagaatatc ggaggcaggt ttctgctcaa gatgcttttt tccaaaccgc atctgggaga
     1801 agccgaggaa ctggccagtt cacaccttgg agtgccggtg cggcttgtga tcatgccgtc
     1861 cgcgggtttt gctcgcaatc tgtcctcaga agaggatctc acggcggccg gcgttttgta
     1921 acgcggcttg atctcgggcg tcgtcacgca ccattcactt ttgtgagtac gagccggcag
     1981 tctgaaaaag ctcgctgaaa gaagggtttc gggtggtgat gaattagcct gggggtggtc
     2041 agggggcccg gtcaggggcc ggtggtcggt tttcactctc gttttcctgg tcgccgcctt
     2101 cgatcccgag cgctttcatt ttgcgctgca gcgtgcgtcg cgagactttg agaacccgcg
     2161 ccgtgagcga cacgttgccg cgattgttct cgagagattg ctgaataatc tctcgctctg
     2221 ccgacatcaa ctgatctttc aggcttcccg tcgaaacgct ggtggaacgg gcgctgccgg
     2281 aacgggccgg cttctcgagc aggttcggcg gcagatggga tggttgaatg accgcatctg
     2341 cgaggaccac cgtacgtttg agcacatttt cgagttcacg aatattgccg ggccagctgt
     2401 aactgaacag cagccgacga gtggcggggt cgagtcgcgg tacggttttt cggccgtttt
     2461 ccttggcgaa tttctcgaga aagtgctcga tcagcaaagg caggtcgcct tgtcgctctc
     2521 tgagcggcgg cagcgtcacg gtcatctcgt tgatgcgata gtacaagtcg tggcggaaac
     2581 tgccctcgcg caccagggtc agcaggtcgc gattggttgc ggcaatgacg cggatgtcga
     2641 cgtcgatcgg gcgggtgccg ccgacgcgac gaatcttctt ttcctgcaga acccgcagga
     2701 gtttgacctg cgtgttgagc gaggtttccg agatctcgtc gagaaagatc gttcccttgt
     2761 cggcgatctc gaaatacccg atcttctggc cgacagcgcc ggtgaacgcg ccgcgctcgt
     2821 gcccgaacag ttcgctctcg atgatggaat caggcagggc gccgcaatta accggaatga
     2881 agttttcctg cgatcggggc gaattcatgt ggatggcgtg agccaccagt tctttgccgg
     2941 tgccgctttc cccctgaatc aggacggtca gattgctggc gctgatacgt tcgaggcacg
     3001 aaaaaatcga ctgcatcttc gccgactgtc cgatgattcc cttgaatcgg gcagagctgc
     3061 tgccaccgga ctctttcgcg gggctggtca tctcgaagat gttgatgggc gagaactcga
     3121 agatgacttt gttcgagatc ttctggatcc attgtggaag caccgtggtg agttcgccga
     3181 tctcgaggtc gctgagttgc agccgacggc cctcacgcac aatgagagct cggacatctt
     3241 cggcgctcat ctgcagcagt tcctcgatgt tgcgctccat gagccgattg agaatggtca
     3301 gataactgag gtagtgcgac gaaccgctgc tgacctctgg tggttcaacg atgccgatgt
     3361 cggcgagcac ctgctgtcgc atatcgggag gcagtgcctg ctcgaagttg acttcctgct
     3421 tgcgcggaaa accggacttc catttctcaa acagtcgatc gagatccttc acgcctcgcg
     3481 gcgctacttc agcgaacagt tgcgtcgcga ggtcgcgaag gtcgtgccag ttttccatga
     3541 aaggactcag tttccctgtt tgatcggggg cttctgggaa ctggcttcgg ggttgacccg
     3601 gccggtgttc cccgtcggtt cggtcgatgg cgacggagtc gctagcgtgt caggtaccac
     3661 ggcggccgga ctatcctgga cgaccggggc gggcttctga tcagccttcg gagttacggc
     3721 cggcttcttg tcgacggctc cggccggctt gggggcgacg agcagatctt tgggcggtgt
     3781 ctttttcaac tgacgcagaa agtggatagc ggtcttgttc cccggatcgc tcttgaggcc
     3841 actgagatag tattcggctg cttccgtggt gtgcttggct tccatggcgc atcgcccagc
     3901 ccacagccag gcctcggtgt tcatcggatt ggctttcgtt gccagcagaa acttttcctg
     3961 agcgcttttc aactgcccct gctggtagaa cagaaacccc cacttgcgca gcgcttccga
     4021 ctggaaaccc tgaatgcggg tcatcttcgg cgtatcggga aactgccgca gaaagtcggc
     4081 ccaggcgttg gcttcgttcg agaagaaacc catctgctga tacaccttgg cgcgccggta
     4141 gagataggtc tcggcgttct gcggatcttt ttcgagaaag tgattcaacc gtgccagact
     4201 gcgttccagg cgagcgcgat cagactggcc gagtcgaagt tcgtcgcgag tgtccgggtc
     4261 gattgtagcc agtacgcgca tgtaggcaag gtcttcacca cacttcgagc aaaagtcggc
     4321 ggaaacctcg ttcatggtgt cacacgccag acagatgttc agtggttcgt cacatttgga
     4381 acaggtcacc tcggcgggtt cggcaacctg accgcagtcg ttgcagatgt tgcggatcgc
     4441 ccaggcattg ccgctggcaa gaagcatggc aagcagagtg agaagagtga ggcatagacg
     4501 ggacacgtgc atgtgagacc tccgaatgta tcgttacgct tgaacggtgc gaatgctgaa
     4561 tgatagaagc ctgtccagtc ggtgtcaacc aacgaagcaa tcccagactg gttcggccga
     4621 catggcttcg agaccggcct tttccagggc cttggcggaa agcaggatgc gtccgtcgtg
     4681 ccagctgccc agggtttcga gaaggggacg gatggcgtcg ccatcttccg gatgtttttc
     4741 cgtgcggaaa atgtaccact cgcgaccatc gaaacacagg acgaattgcc gatcatggaa
     4801 gagaacagag ccgagtcggg gacgctggta actgaacacc ccgtaggaac tggccatgtg
     4861 tgcctgggca aagaccagtt tgggcggcag cggtgtgccg agaaatggca ggtagttgcg
     4921 gttcatcagt tcgggcaatt cttcacgcga ttcggtgatc tcgagtggct cgccgccttc
     4981 gagatccgag gctttcgccc agaaaaacga acgactgcgc ggaatgccca ggcgttcgag
     5041 gtcttcctgc acctcgggaa tatcgcagaa gaacgccagg cccttgtatg agcgcttggc
     5101 ggctgcttcc tgcagaccgt tcagaagttt ccgtcccagt cgcgggtcac gataatccgg
     5161 atgcagacgc cagtgcggca cgagcccttc gccagatggc atcccgatga gatcggcata
     5221 cccgacaatg gtgcgagcca gcgtcaccac ccacgacgaa ccgagggcgc ggcagacgat
     5281 tttctgacgc atgcgcacgg tttcctcgcg gtcgaaccag ctgccttcca gatgttcgcg
     5341 gcagcgctgc atgtcgacga gggcttccgg gtcctcttcg aacgaaaagg cacgaaactg
     5401 agcgagttcg atgatttctg acatgaatgg attatgcctg agaaggggga caaagaacaa
     5461 gacccgcagc cacgatcatg aacccagggg ctatttggcg ctattctccc tgtctgaagc
     5521 gactgatgcg ggcatcttgt cgatctcgcc cttctcgatc aaagcgatca ggcgcagaat
     5581 cagcacatcg aaggtttttg tctccagcgg ctgcgtcggc tggttgatgg tcgcggagag
     5641 gcagtacgtc ggtgaagcgg gagccttctg cagacagatg gtatagttca gaaccccagg
     5701 ctcggaaccg cctttaaaag ccacacgata ccacttgtct ttatccacca gaccggggtt
     5761 gatcgcgagt gatgggtcct cccgaagttc ccggagaatg ccgcagagat tgcgcgtcgt
     5821 catgaaccac tcgatgttct gcacttcgat cggatcttcc ttgatctcgg tgagcaggac
     5881 cgggagcgtt gccatggcag tgagcacctc tcgtcggtgg gcgggttccg tgctcgccag
     5941 atatcgcgtg cgccagatgg ggtcgagata cttcaggcgg aacatttcca tggtgctgag
     6001 aaacggttca ttctggtgcg ggtcaagctt ctcgacggtt tctcgaccga ccaggaagag
     6061 cagatgatcg gtggcggtgt tatcactgcg cgagatcatg aggtttgcca gggtctcgat
     6121 ggtgatgggc gtgccgattg gccagtcttg aaggattccg gaggggaggg acttccaatc
     6181 agggttgagg gcaaccgtct gagaagccga ccattttcct tccctgattc cctgccggag
     6241 ggctttcaga atgtagagtt tgaacgccga acccaccgcc agcggtttgt ctccgttgtg
     6301 atccaggaga atcgtatcct ggtttcgcgt cagagtgatg gcagtcgtac ccggaagcgc
     6361 gcgaaaggtc gtgctgatcg aggcgacgga atcgtgatgg tgctttggag cacccatcca
     6421 gaagccgtca atgcgacctg tctggtcgag gtgaatgcgg cagggagctt cgccgcggtc
     6481 gaaacaaagt gtgtagctgc cgctggcccc agcagagttt ttccaggagc caatctcgga
     6541 atgatacatc gacgcgattt ccttgatctt ggcaggcggc acggcttgga ggaactctgt
     6601 ggtgaaaagt tcgactgggg gcaccgaccc ggtgaagaag gcttccaggg cttcggtttc
     6661 tgaccttatg ctctctgcgc tggctggtga gcagaaaaag gtgagcatga caagcca
//
LOCUS       DAORHD010000068         6694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11317_length_6694_cov_3.736858, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000068 DAORHD010000000
VERSION     DAORHD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11317_length_6694_cov_3.736858"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..758)
                     /gene="rsmG"
                     /locus_tag="PKO06_02535"
     CDS             complement(24..758)
                     /gene="rsmG"
                     /locus_tag="PKO06_02535"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNV68547.1"
                     /translation="MNLIRPLLEPLAIALELPLGEEAYVRFERLYQSMVTDPLYPSVS
                     KISDPSDIVNKHFIDSLAPLCLDLDCFPGQGGRVLDIGCGGGFPILPLAIVLTDLSFC
                     AMDAKDKAVEFVARMATATGLRNLTTRHARAEEAAREKNTRERFDLVICRAVAEVRVL
                     VELCLPLVKVGGCALFYKGPKLDEELAAAMHAFEELQVEPADLTTRTLTPPTLPFERG
                     YVLIRKRKPSPEKYPRRNGVPAAKPL"
     gene            complement(760..2634)
                     /gene="mnmG"
                     /locus_tag="PKO06_02540"
     CDS             complement(760..2634)
                     /gene="mnmG"
                     /locus_tag="PKO06_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNV68548.1"
                     /translation="MSSDAQVIVVGAGHAGCEAALAAARSGARTLLLTINLDMIAWMP
                     CNPSIGGQGKGQLVREIDALGGAMGAAADATHIQARLLNTRKGLAVQAIRVQSDKLAY
                     SAFMRHAVLGCPRLTLQQAMVTELIVENRNVIGVRTSSGDVIHAPTIILAPGTFLRGL
                     IHIGPTRFSGGRAGELAADALGLSLETAGISLMRFKTGTPPRISRASIDVRQLESQPD
                     EPDTPRFSLQSSITPFLSRHACYLTRTTADTHDIIRRYMHESALMAGQIRGTGPRYCP
                     SIEDKIHKFPDKPSHKVFLEPEGAESPEVYLQGLSTSLPERVQELYVRSLPGLSNARI
                     TRPGYAVEYDIIDPTTLYPTLMSKQIGGLFAAGQINGTSGYEEAAAQGLLAGVNASRQ
                     ARGASPIVLDPQTSFTGLMIDEITTRAMEEPYRIFTSRSPFRLNLRMSNAEDRLTRIA
                     HDHELVSAKHFDFIQVRSRQIDDLIKQLEAMKVTFAEWRERNPGSCLTPFDGSMSLSR
                     LFKRPETEFAEVAAYVPGLSDLDSLARLEIEARTKYAGYLVLQEREIRLQQQLSDTPL
                     PTDFFDQLPAGLSCESRQKILAVRPATLGALARIPGVRAGDVSVVLLQLRKTQSPRQV
                     "
     gene            complement(2631..4754)
                     /locus_tag="PKO06_02545"
     CDS             complement(2631..4754)
                     /locus_tag="PKO06_02545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68549.1"
                     /translation="MPPITNKRHQMIINNLKSIMAEVRHKSLIQLSELPDTAIPAAEK
                     AKHYQEACNDSEEIVKATAKRLLAALGGTAPEPPPHPAAPAPAAQPPETTHVAPASHA
                     EPAPAPQPEPTFPSFAEPTPEPPPRPAYVVEAPPLEEPLDPALPALQSLKAIPQKLEH
                     IRFLARNRPGGHLTHLLALAGDEIEEVALTALQTLLNLRDPRIPPQVLVFFNQPGFSS
                     QRRFLVLKIIMETSASLEVGALEHILLTEKDVIVKSGLVKVFARMAKEDGLNTLKLCL
                     FDSDPRVRANTVEVIEEQAIQGCEAEISRLLQDPENRVKVNAAKFLVKSGYSQAFNTL
                     KSMLGSSEVWLRDSVIFALGEIGDQPSLTLLKAALKDPNQGIRLSVLKALAKINNNPS
                     RDSLQGSVDDPDPVVAQVARGLWEKIKNQPPKPPAPPPPPGKPLPTSAPEAVVAAPTA
                     PPPSRPAAPPPVPQPHAPSLRPTAAPARPAPPHTPPAPVAEMPSDPGESAPMSEPTEP
                     PTPPTPPVPPSAPELSLPSLDFNDSAPAEPASPVVPVAPPPKPAVTTPPRPPTPPVAP
                     RPVVQPPSRTMPPTATTPKPPTPPVPPIAPKPAAPPVAITLPEGSPKFQKPRSADVYL
                     KLTSGSPADLDAAMKDLPFILGDDQMTLLQVAVAHDNENVRLNAAKILSRKKGPAVVN
                     LLQILAGDTNTLVSSIAAKALTLLK"
     gene            complement(4760..>6694)
                     /gene="mnmG"
                     /locus_tag="PKO06_02550"
     CDS             complement(4760..>6694)
                     /gene="mnmG"
                     /locus_tag="PKO06_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNV68550.1"
                     /translation="FPRSAENESRETSSVFLHETHFPLIVVGAGHAGCEAALAAARLG
                     VDTLLLTIDLNTIALMPCNPSIGGPGKGHLVREMGVLGAEMPRAIDETCLQLKWLNTS
                     RGPAVRARRAQADKWVYRQRLSKAVFNTPHLTVRQGMAVELLCDKDRVQGLLTETGLR
                     FSCEALVLATGTYLNGRIIIGPQSWSGGPQNQRAAIGLSESLARHGVPLRRLQTATPP
                     RVHRDSLTYARMQELPGDVHAGGFLWENRLRQLPDQLSCHLTFTTAETIEAVRRHISQ
                     SPLVLGNITNEGPKHCPSIDRKVLKFPKIEKHQIFIEPEGRDNVECYLQGLTTSMPAQ
                     AQVELIRTVPGLERAEILRYGYAIEYDALAPGSMRKTMASRHLDGLFTAGQINGTSGY
                     EEAAAQGLLAGINAAARLQGKPPLTLSRTQAYLGVLADDLSLWDHPEPYRMTPANAEF
                     RLSLREDSAEFRLLDSARATGLCSPELICSIETWVNRINREWQRLSELRTSPTAELRA
                     RLTEQGVGGLKKQISALDLLSRPQISYQQLGLFLGPDFQPVLSSPDEISYLENETRYR
                     GYADREQDRMQETRLLESLKLPLPLDHPNLKILSESARQYLTNPRYEDLEQLQRQHCL
                     SRSDLAILLSVFAHPIHFTE"
BASE COUNT         1219 a         1821 c         2264 g         1390 t
ORIGIN      
        1 cccgtgtcag cgatctcgtc tcattagagc ggtttggcgg caggtacgcc attgcgacgc
       61 ggatactttt ccggtgacgg ttttcgcttg cgaatcagca catacccgcg ttcgaaaggg
      121 agggtgggcg gagtgagcgt gcgtgtggtc aggtcagcgg gctcgacctg cagttcttca
      181 aacgcatgca ttgccgccgc cagttcttca tcgagcttgg gacctttgta gaacaacgca
      241 caaccgccaa ctttcaccag aggcagacag agctcgacaa gaactcgaac ttcggccacg
      301 gcccggcaga tgacgagatc gaatcgctcg cgcgtgttct tctcgcgcgc ggcttcctct
      361 gcccgggcgt gccgcgtagt gagatttctg agaccggtcg ccgttgccat gcgggcgacg
      421 aattcgaccg ccttgtcttt ggcatccatc gcgcagaacg agagatccgt gagaacgatg
      481 gcgagaggca gaatgggaaa gccgcccccg cagccgatgt cgagcacgcg ccctccctgg
      541 cccggaaagc agtcgagatc gaggcagagt ggagcgagag aatcgataaa gtgcttgttc
      601 acgatatctg aaggatctga gatcttggac acgcttggat acaaaggatc tgtgaccatc
      661 gactggtaca gccgttcgaa ccgcacgtag gcttcctctc cgagtggcag ttcgagcgcg
      721 atggccagtg gttcgaggag cgggcgaatg aggttcatgt cagacctgcc tgggggattg
      781 agttttgcgc agctgcagca gcacgaccga cacgtctccg gcgcgaactc cggggattct
      841 ggccagcgcg ccgagtgttg cgggcctgac tgccaggatc ttctgtcgtg actcgcatga
      901 gaggcctgcg ggaagctgat cgaagaagtc ggtgggaagg ggggtatcac tcaactgttg
      961 ctgcagacga atctcacgct cctgcaacac cagatagccg gcatacttcg ttctcgcttc
     1021 gatttcgagt cgtgcgagtg aatcgagatc cgacaatccg gggacatacg cggcgacttc
     1081 ggcgaattcc gtctccgggc gtttaaacag acgcgacaac gacatcgaac cgtcgaacgg
     1141 agtgaggcac gagccgggat tacgttcgcg ccattccgcg aacgtgactt tcattgcttc
     1201 gagttgtttg atcagatcat cgatttgcct tgacctgact tgtatgaaat cgaagtgctt
     1261 ggctgaaacc agttcgtgat cgtgtgcgat tcgggtgagt cggtcttccg cgttggacat
     1321 gcgtagattc aggcggaacg gtgatctgga ggtgaagatc cggtaaggct cttccatggc
     1381 gcgtgtggta atctcgtcga tcatcagccc ggtgaacgag gtttgcgggt cgagtacgat
     1441 cggagatgca ccccgtgcct gccgcgaggc attgaccccg gcaagaagac cttgcgccgc
     1501 ggcttcttca taccccgaag tgccgttgat ctgcccggcg gcgaacaggc cgccaatctg
     1561 cttcgacatc agcgtcgggt acaaggtggt tggatcgatg atgtcgtatt cgaccgcgta
     1621 accgggccgg gtgattctgg cgttgctcaa gccgggaagg gaacggacgt agagttcctg
     1681 tacccgttcg ggcaggctcg tggagagccc ctgcagatag acttcaggac tctccgcgcc
     1741 ttccggttcc agaaaaacct tgtgtgatgg cttgtcggga aatttgtgaa tcttgtcttc
     1801 aatcgacgga cagtagcgag gtccggtgcc gcgaatctgc ccggccatca gggccgattc
     1861 gtgcatgtac cgacgaatga tgtcatgtgt gtctgctgtt gtccgagtca gatagcaggc
     1921 atgtcggctg aggaaaggag ttatcgacga ttgcaaagaa aaacgcgggg tgtcgggttc
     1981 gtccggctgc gattcgagtt gtcggacatc gatggatgct cgcgagatcc gtggtggcgt
     2041 tcctgtcttg aaacgcatga gcgaaatgcc tgccgtttcg agactcaacc ccagagcgtc
     2101 agcggcgagc tcaccggcgc ggccgcctga aaatcgcgtt ggcccgatgt gaatcaggcc
     2161 gcgcaagaac gttccaggcg caagaatgat tgttggagca tgtatgacat ctccagagct
     2221 tgtacgaaca ccgatgacgt tccggttctc gacgatcagc tcagtgacca tggcctgctg
     2281 cagcgtcagc cgcggacacc ccaggactgc gtgtcgcatg aaggcactgt aggccagttt
     2341 gtctgactgt acgcgaatgg cctgtaccgc gagtcctttc cgcgtattca ggagccgcgc
     2401 ctgaatgtgc gtggcatcag cggctgcccc catcgcgcca ccgagggcgt cgatttcgcg
     2461 taccagctgg cctttgccct gacccccgat ggaagggttg cagggcatcc aggcaatcat
     2521 gtcgaggttg atcgtcagca gcagagttcg cgctccggaa cgagctgccg ccagcgcggc
     2581 ctcacagccg gcgtggccgg cccccacgac gatgacctga gcgtcggaac tcacttgagc
     2641 agggtaagag cctttgccgc gatcgagctg accagggtat tggtatcgcc ggccagaatc
     2701 tgcaacaggt tgaccacggc cgggcctttt ttgcgagaca gaatcttggc cgcgttcagg
     2761 cgcacgtttt cgttgtcgtg ggcgacggcg acctgaagca aggtcatctg gtcatctccc
     2821 aggatgaacg gcaggtcttt catggccgca tcgaggtcgg cgggcgagcc actggtcagc
     2881 ttgagataca cgtccgccga tcgaggtttc tgaaacttcg gtgacccttc gggaagcgtg
     2941 atggcaacag gcggcgcggc cggtttcgga gcgatggggg gaacgggtgg cgtcggtggc
     3001 tttggtgtcg tggccgtcgg cggcatggtc cgcgagggtg gctgcacgac ggggcgcggc
     3061 gcgactggtg gcgtcggcgg gcgtggcggt gtcgtcacgg cgggcttggg cggtggtgct
     3121 accgggacga ccggagaagc cggttctgcc ggcgcactgt cgttgaaatc gagagagggc
     3181 agcgaaagct ctggtgccga cggtggcacc ggtggcgtcg gcggcgtggg tggttcggtg
     3241 ggctctgaca ttggcgcgct ttcgcctgga tcagacggca tctcggcgac cggggcaggt
     3301 ggtgtgtgtg ggggggcggg ccgcgcggga gcggccgtcg gtcggagact gggagcgtga
     3361 ggctgcggca caggaggcgg ggccgcgggg cgggagggcg gcggtgcggt cggagctgcg
     3421 acaactgctt cgggagcgct tgttggcagc ggcttccccg gtggcggtgg tggtgccggc
     3481 ggttttggcg gctgattctt gatcttttcc cacaacccgc gtgcgacctg ggcgaccacg
     3541 ggatcagggt cgtcgacgga tccctgcagg gaatctcgag aaggattgtt gttgattttg
     3601 gccagggcct tcaggacgct gagacgaatg ccctgattcg gatctttcaa tgccgctttg
     3661 agaagggtga gcgatggctg gtcgccgatt tctccgaggg cgaagatgac gctgtcgcgc
     3721 agccagactt ctgatgaacc gagcatggat ttcagggtgt tgaacgcctg cgaataccct
     3781 gacttgacca gaaacttggc cgcgttcacc ttgacgcggt tttccgggtc ttgcagaagg
     3841 cgggaaatct ctgcctcaca accttgaatg gcttgttcct caatgacttc gacggtgttc
     3901 gctcgcacgc gcgggtcact gtcaaacagg cacagcttca gggtgttgag gccgtcttcc
     3961 ttggccattc gcgcgaaaac cttgacgaga ccagacttca cgatcacatc tttttcggtc
     4021 agcagaatgt gttcgagagc gccgacctcg agtgatgccg atgtttccat gatgatcttc
     4081 aagacgagaa aacggcgctg cgatgagaat ccgggctgat tgaagaagac cagcacctgc
     4141 ggtggaatac gcgggtcgcg caggttcagc agagtctgca gtgccgtcag ggcgacctct
     4201 tcgatttcgt ctccggcgag tgcgagcagg tgcgtcaagt gcccgcccgg gcggttgcgc
     4261 gccagaaagc gaatgtgttc gagtttctgt ggaatggcct tgagcgactg gagtgccggc
     4321 agtgccggat cgagtggctc ttcgagcgga ggggcttcga ccacgtacgc gggtcgtggt
     4381 ggcggctcgg gtgtcggctc ggcgaacgag gggaacgtgg gttcaggctg gggtgccggc
     4441 gcaggttcag cgtggcttgc cggtgcgacg tgcgtggttt ccgggggctg ggcggctggt
     4501 gccggtgcgg cgggatgggg cgggggttcg ggggctgttc cgcccagggc cgcgagcagg
     4561 cgcttggctg tggccttgac gatttcttcg ctgtcgttgc aggcttcctg gtaatgcttc
     4621 gccttttccg ccgccggaat cgcggtgtcg ggcaactctg agagctgaat gagcgacttg
     4681 tgtcgaactt ccgccatgat cgacttcaga ttgttgatga tcatctgatg gcgcttgttc
     4741 gtgataggag gcatggcggc tactccgtga aatgaatcgg atgggcgaag accgataaga
     4801 gaatagcaag atctgagcgg gaaaggcaat gctgtcgctg cagctgctcc agatcctcgt
     4861 aacgtgggtt ggtcagatac tgtcgtgcgc tctcggagag gatcttcaga ttcggatggt
     4921 cgagtggcag gggaagcttg agtgattcga gcagtcgggt ttcctgcatc cgatcctgtt
     4981 cacggtcagc atacccgcga tagcgggttt cgttttcgag ataactgatt tcgtcagggc
     5041 tggacaacac cggttgaaaa tcagggccga gaaagagtcc gagttgttga tacgaaatct
     5101 gcggtctgga aagcagatcg agggctgaga tctgtttctt caagcccccg acaccctgct
     5161 cggtgagccg tgcacgcagt tcggccgtgg gcgaggtgcg cagttctgac agccgctgcc
     5221 actcgcggtt gattcggttg acccacgtct cgatcgaaca gatcaactct ggactgcaca
     5281 accccgtggc cctggcgctg tcgagcagac gaaactccgc ggagtcttcc cgcagcgaca
     5341 ggcgaaactc ggcattggcg ggggtcatgc gatagggctc ggggtgatcc cataaggaga
     5401 ggtcatccgc gagcaccccc agatacgcct gggtgcgtga cagcgtcagc ggcggcttcc
     5461 cctgcagtcg ggcggcggca ttgatgccag ccagcagacc ttgtgccgcg gcttcttcat
     5521 accccgaggt gccgttgatc tgaccggcgg tgaacagtcc gtcgagatgg cgtgacgcca
     5581 tggttttgcg catcgacccc ggcgcgagcg cgtcatactc gatggcatac ccgtagcgca
     5641 gaatctcggc acgttcgagg ccgggaacgg tgcggatcaa ctcgacctgc gcctgagcgg
     5701 gcatgctggt cgtcagaccc tgaagatagc actcgacgtt gtctcggcct tcgggttcga
     5761 taaagatctg atgcttttca atcttgggga acttgagaac cttgcggtcg atcgaggggc
     5821 agtgctttgg cccctcgttg gtgatgttgc cgagcacgag cggggactgg ctgatgtgcc
     5881 gtctgactgc ctcgatggtt tccgcggtgg taaaagtcag atggcagctg agttggtcgg
     5941 ggagctgacg caggcggttt tcccacagaa atcctccggc atgcacatca cccggcagtt
     6001 cctgcatgcg ggcgtaggtg agtgagtcgc gatgcacgcg tggcggagtc gcggtctgca
     6061 gacggcgaag cggcacgccg tgtcgagcaa gcgattcgga cagcccgatc gcggcacgtt
     6121 ggttctgggg tccgcccgac cacgactgcg ggccgatgat gatgcgcccg ttgagatacg
     6181 tgccggtggc gagtaccagt gcctcacagg aaaaccgcag tccggtctcg gtgagcaggc
     6241 cttgaactcg atctttgtcg cagaggagct caacggccat gccctgccgt acggtcaggt
     6301 gcggggtgtt gaacacggct ttggataacc gctgtcgata gacccacttg tccgcctgag
     6361 ctcgccgggc tcgcacggct ggcccgcgcg acgtgttgag ccacttcagc tgcagacagg
     6421 tttcatcgat ggcgcgaggc atttcagcgc cgagaacgcc catctcgcgc accaggtgcc
     6481 ctttcccagg gccgccaatg ctgggattgc atggcatcag cgcgatcgtg ttcaggtcga
     6541 tggtcagcag cagggtgtcg accccgagtc gtgccgctgc cagagcggcc tcgcacccgg
     6601 catggccggc tcccaccacg atcagcggaa aatgagtctc gtgtaggaac acggacgatg
     6661 tctcacgaga ctcattttcc gctgatcgtg gaaa
//
LOCUS       DAORHD010000069         6691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11324_length_6691_cov_3.389542, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000069 DAORHD010000000
VERSION     DAORHD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6691
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11324_length_6691_cov_3.389542"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKO06_02555"
     CDS             complement(<1..454)
                     /locus_tag="PKO06_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68551.1"
                     /translation="MHRRAGLRAFLLLIVFSSLWPLFAAGPEALSVHAVTVDFPLISI
                     RVKLIASGSFDVSASTFAINEGGAAIADFAVASATPRQIMAFVLDRSSSLENQIKDVR
                     RSAVAFLKSLPVGLKIEVLSFASDVEIDQEFTLARNDLISAVEFSRVNL"
     gene            complement(454..2412)
                     /gene="htpG"
                     /locus_tag="PKO06_02560"
     CDS             complement(454..2412)
                     /gene="htpG"
                     /locus_tag="PKO06_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNV68552.1"
                     /translation="MSETNNTFEFKAEAKQLLDLMIHSVYSHKEIFLRELISNASDAL
                     DKLRFESLTNETLRSLAEDLHIRLSADPKARTLSVVDNGIGMSRDDMLAYLGTIAKSG
                     TRDYLRLIQEAKEKVGSPDLIGQFGVGFYSSFMVADKVTVVSRKAGETQAWQWESVGD
                     GRFTITEASRDRQGTTVTLHLKPEDKEDEEAQDFTAEWVIREIVRKYSDFVAYPIKMQ
                     TERTEHERDADGKVKEGTEPKTVIEDQTLNSMKAIWTRPEKEVTEEEYKEFYKHISHD
                     WTEPMKHIVYRAEGAMNDFRSLLFLPSKAPHDLFFPEGSRGIHLYVKRVFIMSDCKEL
                     IPEYLRFVRGVVDSDDLSLNISREILQQNRQIQGIRKSIIKKVLDTLRTMLQEQKDKY
                     LDFWKEFGRVVKEGLFKEPGDRDRIFEVTLLPSTNHATELTTLEEYVGRMKSEQKDIY
                     YLTGESRQILEQSPHLEAFRDKGYEVLLLSDPVDEVWVPFTMEYKGKRFQNIGKGQVN
                     LGSDDEKKKKEEALKEKQDSCKSLFEFMKTHLSEHVKEVRLSSRLTSSPACLVSEVND
                     MSPQLEQLLRASGKDTPHTKRILELNPTHPILDKMQAIHKIDASDARLREYTDLLFGQ
                     AILAEGGKPPEPAKLSKQISELMMKAIG"
     gene            complement(2531..2932)
                     /locus_tag="PKO06_02565"
     CDS             complement(2531..2932)
                     /locus_tag="PKO06_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV68553.1"
                     /translation="MPTFFTIVIVVIILHIVKSMFSWEGEAMNVQQLRDAWANPATRQ
                     ADARLIDVREPGEFSGGHVPGAINIPLASIGNRATELASASTLYVICHSGARSAAACG
                     TLQKQLPATVKIINISGGTAAWQAAGFPVEK"
     gene            complement(2944..3765)
                     /locus_tag="PKO06_02570"
     CDS             complement(2944..3765)
                     /locus_tag="PKO06_02570"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV68554.1"
                     /translation="MQQPDWLDRLERRVPGLAVPGIVRIVTLGMLFAFGLINSGTTPQ
                     EFWLLSGNTLAHGQIWRVFTFLFHPPTFNMILLIFELMILVMCGDGLEEAWGSFRVTV
                     YYLTGAIATVLCGFLVPEMAIPNTYLNLSLFLAFATVYPDYEILLFFIIPVKIKYLAL
                     FSGLLIAWTIITAPLLFKLVAALSIGNYLLFFGPAAFRLAMQTGSANLRRRQFESTMK
                     AAVPPRHVCSVCGRSSQTDPDVFIRYCTCKACGDNGRGFCEEHLQAHEEGNPTIH"
     gene            3869..5404
                     /locus_tag="PKO06_02575"
     CDS             3869..5404
                     /locus_tag="PKO06_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HNV68555.1"
                     /translation="MLEMVDLKKKLEKAEFKKWMDDAERKINELQRRMKDAGVPTIIV
                     FEGLEAAGKGTCINRLMLPLDPRGFKVHPIHPPNVEERLRPFLWRFWNKLPARGRLAI
                     FDHSWYDRCLTERVQKEVSSKDLPKAYEEIQAFERQQTDDGAVLVKFWLHISRKEQRK
                     RFKAILDNPATAWKIGKDEKRQQKQYDKYLEAAEEMFERTNTAFAPWVIVEAHDKQFA
                     IQKIFQTIISVWEAALEKVAAKAAAKASAGAKDGKDKPAKKAPKSLPAKNVTILSALD
                     LTKKVSEKEYDEKVDKYQKRLLELEHEIYVRRIPVVVAFEGCDAAGKGGAIKRLTERL
                     DPRGYEVIPVAAPTREELDHHYLWRFARALPKAGHITIFDRTWYGRVLVERVEGFCSD
                     IAWQRAYREINEFEAHLAHDGTVVVKFWLHIDQDEQMRRFKERQNTPWKQWKITEEDW
                     RNREKWPMYETAVNDMLANCSTSYAPFTIVEANDKLHARLKILKTVIDAIEKALKSGV
                     GKA"
     gene            5434..6354
                     /locus_tag="PKO06_02580"
     CDS             5434..6354
                     /locus_tag="PKO06_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HNV68556.1"
                     /translation="MKLDILPEIREALAARRPVVALESTIIAHGMPYPENLKTAQLLE
                     ATVRENGAVPATIAIIGGVWKIGLSPADLEFLATAKEVRKASRMDLGGVLSRGLHAAV
                     TVCGTMIGADLAGIRIFATGGIGGVHRGATQTFDISADLDELGRTRVAVVSAGAKAIL
                     DLPLTLEYLETKGVPVVGYGTSELPAFYTRESGLKLVERVDTPEQAAALLHAHWQVPH
                     SGGVLIANPIPSEAALPRALIDGAIEEALKQAQRENVAGKALTPFLLGALGKITAGAS
                     LTANIALVKHNAAVSARIATALSAHLKATC"
     gene            complement(6444..>6691)
                     /locus_tag="PKO06_02585"
     CDS             complement(6444..>6691)
                     /locus_tag="PKO06_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV68557.1"
                     /translation="LKNDAALKLYVVGHTDNAGTFDHNVKLSQARAAAVVEALVKKNG
                     IAGTRLTPFGAGPTSPVASNATEEGKAQNRRVELVAQ"
BASE COUNT         1393 a         1871 c         2083 g         1344 t
ORIGIN      
        1 tgaggttcac gcgcgagaac tccaccgccg agatcaggtc gttgcgagcc agcgtgaact
       61 cctggtcgat ctcgacgtcg ctggcgaacg aaagaacttc gatcttgagt ccgaccggca
      121 acgacttcag aaacgcgacc gccgatcggc gcacgtcctt gatctggttc tcgagactcg
      181 acgagcggtc gagcacgaag gccatgatct gccggggcgt ggcactggcg acggcgaagt
      241 ccgcgatcgc cgcgccgcct tcgttaatgg cgaacgtact ggcggaaacg tcgaaagatc
      301 cgctggcaat aagcttgaca cgaatcgaga tcagcgggaa gtcgaccgtc acggcatgca
      361 ccgacagggc ttcgggcccg gccgcgaaga gcggccagag actgctgaag acgatcagca
      421 gcaggaaggc ccgaagcccc gcacgccggt gcatcagccg atggctttca tcatcagctc
      481 ggagatctgt ttgctgagct tggcgggctc gggcggcttg ccgccctcgg cgagaatcgc
      541 ctgcccgaac agcaggtcag tgtactcgcg caaccgcgca tcgctggcgt cgatcttgtg
      601 aatcgcctgc atcttgtcga gaatcgggtg cgttggattc agctcgagaa tgcgcttggt
      661 atgcggcgta tctttgcccg aggcgcgcaa cagctgttcg agctgcgggc tcatgtcgtt
      721 gacttcgctc accagacagg ccggtgaaga ggtcaggcgg ctcgacaggc gcacttcctt
      781 cacgtgttcg ctcaagtgcg ttttcatgaa ctcgaacagc gacttgcagg aatcctgctt
      841 ctctttcagc gcttcttctt tcttcttctt ctcgtcgtca ctgcccagat tcacctggcc
      901 cttgccgatg ttctggaacc gcttgccctt gtactccatg gtgaacggca cccagacctc
      961 gtcgaccggg tcgctcagca acagcacttc gtagcccttg tcgcggaacg cttcgaggtg
     1021 gggcgactgt tcgagaatct gccgcgactc gccggtgaga tagtagatat ctttctgttc
     1081 agatttcatg cgcccgacat actcttccag agtggttaac tcggtggcgt gattggtact
     1141 gggcaacaga gtgacctcga agatacggtc gcgatcgccc ggttccttga acagaccttc
     1201 cttcacgacg cggccgaact ccttccagaa gtcgagatac ttgtctttct gctcctgcag
     1261 catggtgcgc agggtgtcga gcaccttctt gatgatcgat ttgcgaatgc cctgaatctg
     1321 ccggttctgc tgcagaatct cgcgcgagat gttcagcgac agatcgtccg aatcgacgac
     1381 gccgcgcacg aaccgcagat attccggaat cagttccttg cagtcgctca tgatgaagac
     1441 gcgcttgaca tagaggtgaa tgccgcgact gccttcgggg aagaacagat cgtgcggggc
     1501 cttcgacggc aggaacagca gtgagcggaa atcgttcatc gcgccctcgg cccgatagac
     1561 gatgtgtttc atcggttcgg tccagtcgtg gctgatgtgc ttgtagaact ccttgtactc
     1621 ttcttcggtg acctctttct cggggcgggt ccagattgcc ttcatcgagt tgagcgtctg
     1681 gtcttcgata acggtcttcg gctcggtgcc ttccttgacc ttgccgtcgg catcgcgctc
     1741 gtgttcggtg cgttcggtct gcatcttgat cgggtaggcg acgaaatcgg aatacttgcg
     1801 cacgatctcg cgaatcaccc actcggcggt gaagtcctgt gcttcctcat cttccttgtc
     1861 ttcgggcttc agatgcagag tgacggtggt accttgccgg tcgcgggaag cttcggtgat
     1921 ggtgaaccgg ccgtcgccga cggattccca ctgccaggcc tgggtctcgc ccgccttgcg
     1981 gctgacgacg gtgactttgt cggcgaccat gaagctcgag tagaacccga cgccgaactg
     2041 gccgatcaga tcgggcgagc cgaccttttc cttcgcctcc tggatcagcc gcagataatc
     2101 gcgggtgccg gacttggcga tagtaccaag atacgccagc atgtcgtcgc ggctcatgcc
     2161 gatgccgttg tccacgacac tgagcgtgcg ggctttcggg tcggcagaca gtcgaatatg
     2221 cagatcttcg gcgagggaac gcagtgtttc gttggtcaga gattcgaagc gcagcttgtc
     2281 aagagcgtct gaagcgttcg agatgagttc gcgcagaaag atttccttgt gggaatagac
     2341 cgaatggatc atcagatcga gcagttgctt ggcttcggcc ttgaattcaa aggtgttatt
     2401 cgtttcgctc atggagagac ctcctgtgat gtatgttcat gacgttttac ctgtaccagg
     2461 tcgctggtgc aaccggcagg tgattctgct gccttagact cagaccggcg ggtgcgcgtt
     2521 cccggaaaaa ctacttctcg accgggaaac ccgcggcttg ccaggcggcc gtcccgccgg
     2581 aaatgttgat gatcttgacc gtggctggca gttgcttctg gagcgttccg caggccgcgg
     2641 ccgaacgggc accggaatga cagatgacgt acagcgttga agccgaggcg agttctgtgg
     2701 cgcggttgcc gatcgaggcc aagggaatat tgatcgcccc ggggacatgg ccaccggaaa
     2761 actcaccagg ctcgcgaaca tcgatcagac gcgcatccgc ctgacgagtc gcgggattcg
     2821 cccaggcgtc acgaagttgc tgaacattca tggcttcacc ttcccagctg aacatggatt
     2881 tgacgatgtg cagaatgatg acgacgatga caatggtaaa aaaagtgggc aagttagcgc
     2941 ctttcaatgg atggtcggat tcccttcttc gtgggcctgc agatgctctt cacagaatcc
     3001 gcggccgttg tcaccacagg ccttgcaggt gcagtagcgg ataaacacgt cggggtctgt
     3061 ctgagagctg cgaccgcaca cggaacacac gtgacgcgga ggcacggctg ctttcatggt
     3121 gctttcgaac tggcggcgcc gcagattggc tgaaccggtc tgcatcgcga gtctgaaggc
     3181 cgcggggcca aagaaaagca ggtagttgcc gatcgacagg gccgcgacga gtttgaacag
     3241 cagcggcgcg gtgatgatgg tccaggcgat cagcagcccg gaaaagaggg ccagatactt
     3301 gatcttcacc ggaatgatga agaagagcag aatctcgtaa tcagggtaga ccgtggcgaa
     3361 ggccagaaac agcgacaggt tcagataggt gttgggaatc gccatttccg gaacgagaaa
     3421 cccgcacaga acggtcgcga tagcccccgt cagatagtac acggtgacgc gaaaactgcc
     3481 ccaggcctct tcaagaccgt cgccgcacat gaccagaatc atcagctcga agatcagcag
     3541 aatcatgttg aacgtcggcg ggtgaaacag aaacgtgaag acgcgccaga tctgcccgtg
     3601 cgccaaagtg ttcccggaca gcagccagaa ctcctgcggc gtggttccgg agttgatcag
     3661 gccgaaggcg aagagcatgc cgagcgtgac gatacgaacg atcccgggta cggccagtcc
     3721 gggcacgcga cgttcgagtc ggtcaagcca gtcaggttgt tgcatggcgg gtttcagtgg
     3781 cctcgacaag ggaaatcatg cgcagcaagt cggcgtatgg tagcatactt cgacgtacgt
     3841 cgactatgta ccagggagga cgagagcaat gcttgaaatg gtggatctga agaagaaact
     3901 cgagaaagcc gagtttaaga aatggatgga cgacgcggag cgcaagatca acgagctgca
     3961 gcgtcgcatg aaagacgccg gggttccgac gatcattgtg ttcgagggtc tggaagcggc
     4021 ggggaagggc acctgcatca atcggttgat gttgccgctt gatccgcgcg gtttcaaggt
     4081 tcaccccatt cacccgccca acgtggaaga acgactgcgt cccttcctgt ggcgcttctg
     4141 gaacaagctg cccgcgcgcg gccgcctcgc catctttgat cattcctggt atgaccgatg
     4201 tctgaccgag cgcgttcaga aagaggtgtc gagcaaagat ctgcccaaag cgtatgaaga
     4261 gattcaggcg ttcgagcgcc agcagactga cgacggcgcg gtgctggtca agttctggct
     4321 gcatatcagc cggaaggaac agcgcaagcg cttcaaggcg attctcgaca acccggccac
     4381 ggcctggaag atcgggaagg atgaaaagcg gcagcagaag caatatgaca agtatctcga
     4441 agccgccgaa gagatgtttg agcgcacgaa tacggccttc gccccctggg tgatcgtcga
     4501 ggcgcacgac aaacagttcg cgatacagaa gatttttcag accatcattt cggtctggga
     4561 agccgccctc gagaaggtgg ctgccaaggc cgcggccaaa gcctctgccg gtgccaaaga
     4621 cggcaaggac aaacccgcga agaaagcgcc gaagtcgctg ccggccaaga atgtgacgat
     4681 tctctcggct ctcgatctga ccaaaaaggt gtcggaaaaa gagtatgacg agaaggtcga
     4741 caagtatcag aagcgtctgc tcgaactcga acacgagatc tacgtgcggc gcattccggt
     4801 ggtcgtggcg tttgaaggct gcgacgccgc cggcaagggc ggggcgatca agcgtctgac
     4861 cgagcggctc gatccgcgtg gctacgaggt tattccggtc gctgcgccga cccgcgagga
     4921 actcgatcat cattatctgt ggcgatttgc ccgcgcgctg ccgaaagccg gacatatcac
     4981 gatcttcgat cgcacctggt acggtcgggt gctggtcgag cgcgtcgaag gtttctgctc
     5041 tgatatcgcg tggcagagag cctaccgtga aatcaacgag ttcgaagctc atctggccca
     5101 tgacggcacg gtcgtcgtca agttctggct gcatatcgac caggacgaac agatgagacg
     5161 gttcaaggaa cgccagaaca ccccctggaa acagtggaag atcaccgaag aagactggcg
     5221 caatcgcgag aagtggccca tgtacgagac ggcggtcaat gatatgctgg ccaactgctc
     5281 gaccagctat gcgccgttta ccatcgtgga agcgaatgac aaactgcacg ctcggctcaa
     5341 gattctcaag accgtcatcg acgcaattga aaaggcattg aaatcaggag tcggcaaggc
     5401 ctaagcctga cagcaactcg tacggagcac atcatgaaac tcgacattct gcctgagatc
     5461 cgcgaagccc tggcggcgcg cagaccggtc gtcgcgctgg aatcgacgat tatcgcgcac
     5521 ggcatgccgt atccggagaa tctgaagacg gcgcagcttc tggaagcgac ggtgcgcgag
     5581 aacggcgcgg tgccggcgac gatcgcgatt atcggcggtg tctggaagat cgggttgtcg
     5641 cccgccgatc tcgagtttct ggcgaccgcg aaagaggtgc gcaaggcgag ccgcatggat
     5701 ctcggtgggg tgctgagtcg cggcttgcat gccgccgtca cggtctgcgg cacgatgatc
     5761 ggtgccgacc ttgcgggcat tcgcatcttc gcgaccggtg gcatcggcgg cgtgcatcgc
     5821 ggcgccacac agaccttcga tatctcggct gatctcgacg agctcggtcg aacgagagtc
     5881 gccgtcgtct cggccggggc caaggcgatt ctcgatctgc cgctgacgct cgagtatctc
     5941 gagacgaaag gtgtgcccgt tgtcggctat gggaccagtg agctgcccgc gttctatacg
     6001 cgcgaaagcg gtttgaagct ggtagaacgg gtcgatacgc cggaacaggc cgcggcattg
     6061 ctgcacgcgc actggcaggt gccgcattcc ggcggggtgc tgatcgccaa tccgattccg
     6121 tccgaggcgg ccctgccgcg agcgttgatt gacggcgcga ttgaagaggc cttgaaacag
     6181 gctcagcgag agaatgtcgc cggcaaggcc ctgacgccgt ttctgctggg ggcactcggc
     6241 aagatcactg ccggcgccag tctgaccgcc aacattgcac tggtgaaaca caatgctgcc
     6301 gtgtctgctc gcattgccac ggcgttgtct gcccatctca aggccacctg ctgacgttca
     6361 ccggtttttg tccgagaaac gaacagcgcc acaaagcctg aaaagctgct ttgcggcgct
     6421 gtttgttgca gccggttgtt gggttactga gcgacgagtt ccacgcggcg attctgggct
     6481 ttgccttctt cggtggcgtt cgaggcgaca ggagaggtcg gtcccgcgcc aaacggggtc
     6541 aggcgagtgc cggcaatgcc gttctttttc accagagcct cgacgacggc cgccgcccgg
     6601 gcctgcgaga gcttgacgtt gtggtcgaag gtgccggcat tgtcggtgtg tccgaccacg
     6661 tagagtttga gagctgcgtc gttcttgagc a
//
LOCUS       DAORHD010000070         6672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11376_length_6672_cov_3.393532, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000070 DAORHD010000000
VERSION     DAORHD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11376_length_6672_cov_3.393532"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="PKO06_02590"
     CDS             <1..1474
                     /locus_tag="PKO06_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68558.1"
                     /translation="ALAGIWAGLFLLLQMRVIDDMDILLQIVLGRHFIEHGELPSTEP
                     LCFSLSGSVFVPLGWLAQVFAALIDRIGGLYLLHTLRSVLYATALLLAATLSLTGQTR
                     KPVGILLGIALAFPVFMTSANIRPQAAILPLFFLILHLANSSFLLWKKIIVTAAIGVL
                     WQNLHASVPAGIAALVLLSVGNFLNGRWSPPTSPEAGPQAVPWSDAAILALILAGTQL
                     CTPLGLDIFALSRLNYTISAQWLGISEWLPAWHSSMVMHLAPFWAALMLTLAVLWLRP
                     TLRHPGEMALALPFAGLTLLSCRFVFFWAPLMVPIWARWADLLWEHRASSIPTSSESP
                     SDSTTIPTVVVLLMILTGCLLPRYFSPLDRSSVPRQAVQQLVQQISFGRVYNFREWSG
                     PLVYFSAGALKPAIDGRLYLFAYQTWLEYQQAASGEIAVASLTRKFAPDAFFLHPTYH
                     AALTTLLRHDPAWRQIWADEQAVVFVPALSPPNPELRPSP"
     gene            1588..2655
                     /locus_tag="PKO06_02595"
     CDS             1588..2655
                     /locus_tag="PKO06_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNV68559.1"
                     /translation="MGTRPVLIVSDSLVRAALFEPPFSLSPEGVVLTGFASLRFLALE
                     RYPAVVLHEPPADLLRKLALGIHDQPDGDGLWQALNQVPRVRLYQLVVTPTRPGKDAV
                     ALDIENIVPADLGRLVGEAQRHPQAGVGTPTPGTDRPAQALTADDIRERFQRGERSLS
                     AATPMTDWARETAAALGMRLDETPAGYRLLRVKAFSRRDLEATKDLLFQVSTRYVDAL
                     FIVDLPYLPIVRDLVPSLRGRLVAPRVHWADYGAYTGEVSVEMLTDMDCRGALVPDTP
                     PWTKPEHLQKLVQSAMKRRLLLFLPETLARAAGCDIMTPLPDGTSAVTTCFKLVDGRE
                     NPPPEPPPGTAVLMTSDQLSS"
     gene            2672..3316
                     /locus_tag="PKO06_02600"
     CDS             2672..3316
                     /locus_tag="PKO06_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNV68560.1"
                     /translation="MIDETKVKQIVAEVLKQLDLQTRVKPGTVPVGISNRHCHVSQAD
                     LETLFGAGYQLKNMKNLSQPGQFAAEECVTLVGRKGTLEKVRILGPCRGGTQVEISWT
                     DSYALGVPAVVRDSGDTKSTPGAILLGPKGHVVLKEGVIVASRHIHMHTKDAEKMGLK
                     DNDRVSVKTPGPRSIIFNNVLIRVSEKFALDFHLDTDEANAAGLKNGDAVEVIS"
     gene            3390..3692
                     /gene="eutM"
                     /locus_tag="PKO06_02605"
     CDS             3390..3692
                     /gene="eutM"
                     /locus_tag="PKO06_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007625965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HNV68561.1"
                     /translation="MTQEALGMIETKGLTALIEASDAMVKAANVRLVGWEKIGSGYVT
                     AFVRGDVAAVKAATDAGAAAAKKIGELVCVHVIPRPHANLDEVMPIREETLANAKK"
     gene            3708..3992
                     /locus_tag="PKO06_02610"
     CDS             3708..3992
                     /locus_tag="PKO06_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNV68562.1"
                     /translation="MILGRVCGTVVCTRKDDRLKGMKLLIVQELTIDGKPTANHLVAA
                     DAVSAGAGETVLLVAGSSARLTERTLNCPVDAAICGIVDQVVMDSDRQTR"
     gene            4116..5525
                     /locus_tag="PKO06_02615"
     CDS             4116..5525
                     /locus_tag="PKO06_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HNV68563.1"
                     /translation="MVSDKDINVIVSEVIKRLGVETGSVTVAASRAAGRGVFDNLEEA
                     FKAATEAQKQLVALPLATREAIIANMRKRAMERVEELARVGNEETGYGRVEDKIQKNK
                     LAITKTPGIEDLRPAAYSGDHGLTLQELAPYGLIGAITPSTNPSETVICNSIGMVAAG
                     NAVAFGPHPSAARVSQLAMTILNDAIVEAGGPENLLVCAAKPSLENAQLLFTHPLIRL
                     LVVTGGPAVVAAAAKSGKKFIAAGPGNPPAVVDETADLKKAARDIIAGATLDNNVLCI
                     AEKEIIVVEAVADELKKHLRNSGAYEVSARELVQLEKLVLDHKTGGPNKKFVGKNADF
                     ILEHLGVRVPPDVRMVLAETPPDHPFVVEEMLMPVVPLVRVRDVFAAIELAVKVEHGY
                     HHTAVMHSKNIDNLHLMAVRCDCSIFVKNGPSYAGLGFGGEGPTTFTIASPTGEGITT
                     ARSFTRSRRCVLVDHFRIV"
     gene            5531..>6672
                     /locus_tag="PKO06_02620"
     CDS             5531..>6672
                     /locus_tag="PKO06_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNV68564.1"
                     /translation="MATPSLEALAELGLVGCGGAGFPTQVKLAAKNIDHFIVNGAECE
                     PLLHKDKEILKHFTDEFYAGLSLAMQLTGAKQAHIAVKKKYADLVKHLEKTLPDPRMK
                     VLPLGDFYPAGDEYELVYEAAQRLIPFGGIPLNVGCVVSNVETLTLLGRGKPFTHKIF
                     TIAGAVPNPVTVSVPVGITVREALALAGLTKTDGLTVIDGGPMMGRIVKSLDEIVSKT
                     CGGLIALPTTHPLIFKMTRPEKHQRHIARSACDQCTDCTELCPRRLLGYPIKPHKAMR
                     TSQLAEFHDSNYALDAIFCCECGLCSLYACPEDLPPREISVMAKRHHLAKGVKQTDFK
                     GEPEIHPMRSARRVSIERLIRRLGLEEFDHKAPYREIPLRPEKVTLP"
BASE COUNT         1253 a         2217 c         1864 g         1338 t
ORIGIN      
        1 cgccctcgcc ggcatctggg ccggactgtt tctgctgctg cagatgcgtg tgatcgacga
       61 catggatatc ctgctccaga tcgtcctggg tcggcacttc atcgaacacg gggagctgcc
      121 ttccactgag ccgctctgtt tctcgctttc cggctctgtc ttcgtcccat tgggctggct
      181 cgctcaagtg ttcgccgcgc tgatcgaccg cattggtggc ttgtacttgt tgcacactct
      241 ccgttccgtc ctgtatgcca ccgccctgct gctggccgcc acgctgtctc tcaccggaca
      301 aacgcgcaag cctgtcggca tcctgctcgg catcgctctg gcatttcccg ttttcatgac
      361 cagcgccaat attcgcccgc aggccgcgat tctgcctctc ttctttctca tcctgcatct
      421 cgcgaattcg tcattcctcc tgtggaaaaa gatcatcgtg accgccgcga tcggcgtgct
      481 ctggcagaac ctgcatgcgt cggtgccagc cggtatcgcc gcactcgtgc ttctcagcgt
      541 gggaaacttt ctcaacggtc gctggtcacc gcccacctct cccgaagcag gtccccaggc
      601 ggttccgtgg tctgatgcgg ccatacttgc tctgatactt gccggcacac aactgtgcac
      661 cccgcttggt cttgatatct tcgccctgag ccgcctgaac tacacgatct cggctcagtg
      721 gctcggaatc agcgaatggc ttcccgcttg gcattcgagc atggtcatgc acctggctcc
      781 gttctgggcc gctctgatgc tgacactcgc tgttctgtgg ctgcgcccga ctctgcgcca
      841 tcccggcgaa atggcactgg ccctgccgtt tgccgggctc actctccttt cctgccgctt
      901 cgttttcttt tgggcgccgc tcatggtgcc gatctgggcg cggtgggccg atctgctctg
      961 ggaacaccgc gcctcgagca ttccgacctc gtccgagtcg ccttcagact ccacgaccat
     1021 tccaaccgtc gtcgtcctgc tgatgatcct gaccggctgc ctgcttccgc gatatttctc
     1081 acctctcgat cgctcgtcgg ttccccgaca ggctgtgcag caacttgtac agcagatttc
     1141 ctttgggcgg gtgtacaact ttcgcgaatg gagcgggcca ttggtctact tttccgccgg
     1201 tgctctgaaa ccggcgattg acgggcgcct ctacctgttc gcgtatcaga cctggctcga
     1261 gtatcagcag gccgcgagtg gcgaaatcgc cgttgccagc ctgacacgaa agtttgcccc
     1321 cgacgcgttt ttccttcatc caacctatca cgcagcgctc acgactctcc ttcggcacga
     1381 tccggcatgg cggcagatct gggcggatga gcaggccgtc gtcttcgttc ccgccctttc
     1441 cccgccgaac cccgaacttc gcccgtcgcc ctgagacaag cacgcggcgc cggcccaaac
     1501 gaccatcgcc cggaaagacc atgctcgtct gctcaatgaa aaacagttgt tgcggcttcg
     1561 ggccggttgg gttatgatct cgtcaacatg ggaacacgac cggttctgat cgtttcggac
     1621 agcctcgtgc gcgcggccct tttcgagccg ccgttttccc tgtcacctga aggcgtcgtg
     1681 ctcaccgggt tcgcgtcact gcgttttctc gcgctcgaac gctatccggc cgtagttctg
     1741 catgagccgc ccgccgacct gttgcgcaaa ctcgcgctcg gcattcacga tcaacccgac
     1801 ggcgacggtc tctggcaggc actcaatcag gtgcctcgag tcagattgta ccagttggtc
     1861 gtcacaccca cccgccccgg aaaagatgcc gtggcgctcg acattgagaa cattgtccct
     1921 gctgatctgg gccgtctggt cggcgaagcc cagcgccacc cccaggccgg ggtcggcacc
     1981 ccgacacctg gcaccgatcg acccgcgcag gccctgaccg ccgacgacat tcgtgagcgc
     2041 tttcagcgcg gtgagcgaag tctgtcggcg gcaacgccta tgaccgattg ggcgcgagaa
     2101 accgcggcgg cgctcggcat gcgccttgac gaaaccccgg cagggtaccg gttgttgcgg
     2161 gtgaaagcct tttctcgtcg cgatctcgaa gccacgaaag accttctgtt ccaggtcagc
     2221 acgcggtatg tcgatgcgct gtttatcgtc gacctcccct atctgccgat cgttcgcgat
     2281 ctcgtgccgt cgctgcgcgg gcggctcgtt gcccctcgcg tgcactgggc cgactacggc
     2341 gcgtacaccg gcgaagtctc ggtcgaaatg ctgaccgata tggattgccg cggagctctg
     2401 gttcccgaca cccccccctg gaccaagccg gaacacctgc agaaactggt acaatcggcg
     2461 atgaagcgcc gactgctgct ctttctcccc gaaacccttg cacgagcggc ggggtgtgat
     2521 ataatgaccc cgcttccgga tggtacgtcc gcagtcacca cttgcttcaa actggtcgat
     2581 ggccgcgaga acccaccacc agagccacct ccgggcacgg cggtgctgat gacgtcagac
     2641 cagctgtcaa gctgaaggaa aggaaataga catgatcgac gaaaccaagg tcaaacagat
     2701 tgtcgccgag gtgctcaagc agctcgacct gcagacgcgg gtgaaacccg gtaccgttcc
     2761 ggtcggcatc agcaaccgtc attgccatgt ctcccaggcc gatctcgaaa ccctcttcgg
     2821 cgccgggtac cagttgaaaa acatgaaaaa cctctctcag cccgggcagt tcgccgctga
     2881 agagtgtgtc actctcgtcg gccgcaaggg taccctcgag aaggttcgca ttctcgggcc
     2941 ctgccgcggc ggcactcagg tcgaaatctc ctggaccgac agctacgccc tcggcgttcc
     3001 cgccgtggtc cgcgattccg gcgacaccaa aagcacgccg ggcgcgattc tgctcgggcc
     3061 caagggtcac gtggtattga aggaaggcgt catcgtcgcc tcccgccaca ttcacatgca
     3121 taccaaggat gccgagaaga tgggcctgaa agacaatgac cgcgtctcgg tcaagacacc
     3181 cggtccgcgc tcgatcatct tcaacaacgt gttgatccgc gtcagcgaaa agttcgcgct
     3241 cgacttccat ctcgataccg acgaagccaa tgccgccggt ctcaagaacg gcgatgccgt
     3301 cgaagtcatt tcctaactac gtgtaagaga ctttcccttt gtctacaaaa aaggaaaaac
     3361 caatgtccac aaaatctcag gaggtcccca tgactcaaga ggctctcggc atgattgaaa
     3421 ccaaaggctt gaccgcgttg atcgaagcga gcgacgcgat ggtgaaggcc gccaacgttc
     3481 gtctggtcgg ctgggaaaag atcggttccg gttacgtgac cgcgttcgtt cgcggtgatg
     3541 tcgccgccgt gaaagccgcc accgacgccg gtgccgccgc tgccaagaag atcggcgaac
     3601 tggtctgcgt gcacgtgatt ccgcgcccgc atgcgaatct ggatgaagtc atgccgattc
     3661 gcgaagaaac gctcgccaac gccaagaagt aatcaccgag cgatctcatg attctgggtc
     3721 gtgtttgtgg aaccgtcgtc tgcacccgta aggacgatcg cctgaaaggg atgaaactcc
     3781 tgatcgttca ggagctcacc atcgatggca aaccgaccgc caaccatctg gtggcggccg
     3841 atgccgtcag tgcgggtgca ggcgaaactg ttctgctcgt cgcgggcagc tctgcccgtc
     3901 tcaccgagcg cacgctgaac tgcccggtcg atgcggccat ctgcggcatc gtcgaccagg
     3961 tcgtcatgga ttccgatcgc cagacccgat agatcgccgg tttttccact tcccaacctg
     4021 tacatcttcg ttcggttcgc gttttttgta ccgaatgttg atgtggcttt ttcgcctgca
     4081 ctgcgtgtga caggccacgc gagggggttc atctgatggt ttccgacaag gatatcaatg
     4141 tcatcgtctc tgaagtgatc aaacgcctgg gcgtcgagac cggctccgtg actgtcgccg
     4201 catcgcgggc cgccggccgc ggggtgttcg acaacctcga agaagctttc aaggccgcga
     4261 ccgaagcgca gaagcagcta gtcgccctgc cgctcgccac gcgcgaagcc atcatcgcca
     4321 acatgcgcaa acgcgccatg gaacgcgtgg aagaactggc tcgcgtcggc aatgaagaaa
     4381 ccgggtatgg tcgcgtcgag gacaagattc agaagaacaa gctggcgatc accaagaccc
     4441 ccggcatcga agatctgcga cccgctgcct acagtggtga tcacggtctg acgctgcagg
     4501 aactcgcacc ctacggattg atcggagcga ttaccccctc gaccaatcct tccgaaaccg
     4561 tcatctgcaa ctcgatcggc atggtcgccg cgggcaacgc ggtcgctttc ggccctcacc
     4621 catccgctgc tcgtgtgtcc cagttggcca tgaccattct caacgatgcc atcgtcgaag
     4681 ccggtggccc cgagaacctg ctggtctgtg ccgccaaacc gtcactcgag aacgcccagc
     4741 tgctgttcac gcatccgttg atcagactgt tggtcgtcac cggtggcccc gccgtcgttg
     4801 ccgccgccgc caagagcggg aagaagttca tcgccgccgg ccccggcaac ccgcccgccg
     4861 tcgtcgacga aaccgccgac ctgaagaaag ccgctcgcga tatcatcgcc ggcgctacgc
     4921 tcgacaacaa cgtgctctgt atcgccgaga aggagatcat cgtcgtcgag gcggtcgcgg
     4981 acgaactgaa gaagcacctg cgcaacagcg gcgcttacga agtttccgcc cgcgagctgg
     5041 tacaactcga gaagctcgta ctcgaccaca agaccggcgg accgaacaag aagttcgtcg
     5101 gcaagaatgc cgatttcatc ctcgaacacc tcggagtgcg cgtgccgccc gatgttcgca
     5161 tggtgctcgc cgaaactcca cccgatcatc cgttcgtggt cgaagaaatg ctcatgcccg
     5221 tcgtaccgct ggtacgcgtt cgggatgtct tcgccgcgat cgagctggcc gtcaaggtcg
     5281 aacatggcta tcaccacacc gccgtcatgc acagcaagaa catcgacaac ctgcatctga
     5341 tggccgtgcg ctgcgactgc tcgatcttcg tgaagaacgg cccctcctat gccggactcg
     5401 ggttcggcgg cgaaggcccc actaccttca cgatcgcctc gcccaccggt gaaggcatta
     5461 cgaccgcccg cagtttcacg cgctcacgcc gctgcgtgct cgtcgatcac ttccgcatcg
     5521 tctgatccgc atggccacac cttctctcga agctctggcc gaactgggtc tcgtcgggtg
     5581 cggcggcgcc gggttcccga cccaggtgaa gctcgccgcg aagaacatcg atcacttcat
     5641 cgtcaacggc gcggaatgcg agccgctgct gcataaagac aaagagatcc tcaagcattt
     5701 caccgacgag ttctacgccg gcctctcgct cgccatgcag ctgaccggag cgaaacaggc
     5761 gcacatcgcg gtcaaaaaga agtatgccga tctggtaaaa catctcgaaa agacacttcc
     5821 cgacccgcgc atgaaggtgc tgccgcttgg cgacttctac ccggccggcg atgagtacga
     5881 gttggtgtat gaggccgcgc agcgattgat cccattcggc ggtattccgt tgaacgtcgg
     5941 ctgtgtcgtc agcaatgtcg aaaccctgac cttgctcggt cgcggcaaac cgttcacgca
     6001 caagattttc acgattgccg gcgcggtgcc gaacccggtg acggtttccg taccggttgg
     6061 catcaccgtg cgcgaagctc tggcgctcgc cgggctcacc aagaccgacg gtctgaccgt
     6121 catcgacggc ggcccgatga tggggcgcat cgtcaaatcg ctcgacgaga tcgtcagcaa
     6181 gacctgcggc gggcttatcg cgcttccgac cacgcatccg ctgatcttca agatgacccg
     6241 ccccgagaag catcagcggc acatcgcccg ctcggcctgc gaccagtgta ccgactgcac
     6301 cgaactgtgt ccgcgccgtc tgcttgggta tccgatcaaa cctcacaagg cgatgcgcac
     6361 gtcgcaactg gccgagtttc acgactcgaa ctacgcgctc gacgccatct tctgctgtga
     6421 atgcggcctc tgctcccttt acgcctgccc cgaagatctg ccgccacgcg agatctcggt
     6481 catggccaag cggcaccacc tcgccaaggg cgtcaaacaa accgatttca agggcgagcc
     6541 cgagattcac ccgatgcggt ccgcccgccg cgtcagtatc gagcgcttga ttcgccgtct
     6601 gggtctcgaa gagttcgatc acaaggctcc gtatcgcgag attccgctgc gccccgagaa
     6661 agtgacgctg cc
//
LOCUS       DAORHD010000071         6656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11417_length_6656_cov_3.999849, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000071 DAORHD010000000
VERSION     DAORHD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11417_length_6656_cov_3.999849"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PKO06_02625"
     CDS             <1..414
                     /locus_tag="PKO06_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68565.1"
                     /translation="SMFHDLTINSLDIADLILLMLVPNMNHLKTTHVCLQVMENLKYP
                     KEKIKLLLNRANCERSMNREELETGLKRKIDFTMSDDFQNVSELIEHQKTVYELNNES
                     PYREDMNRLIEVITGKKADAAKKGLTGLLKSFFGQ"
     gene            575..3328
                     /locus_tag="PKO06_02630"
     CDS             575..3328
                     /locus_tag="PKO06_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68566.1"
                     /translation="MTPAVTRTGPTSSVPVLIGLWVFLVLIPGLFFIWMSSQLHEQWR
                     FNEIHTRQNALERSVRRLLRLAHPSQDALEKLAAHPDFPSSPPPLSLSPQGREEHAVL
                     SLLHQFLTDGQSLSQDSQLRICHHFGWVFNPYQVLEQPGVPVEITRAGQSAWLVWSAA
                     TATQPVRIALSAATNAFPRLSDLCSQQFRDTTREWLVIESTGGQATGVPSLRPAIPDL
                     LALAKRQQNGRWQADEHVGISDMAQDGTTVFLRDQLSPRARLSFRSRLHAVLLTLLVI
                     VSCVFPALCRTYLALPLFWKLLMLLGYLVVLPLAASGFLGQGFFHSRALNHAELLRTR
                     ALSDLEVFDSGFLHSIQSLESRFQVLKEALDRRRLSSAQVQKFLDPFFQAQVIDRFLL
                     LSHRGDILLDRCSSRRNHSLMNEVGMAFCRDLFAQRRLGGRKVEEPKRFTLFNDLIQN
                     SIWGSHYLVEGRLQRFLHGTGVLYSYWTLLAPQNPTFGLITLDLFHDGLVTRYLRTTP
                     LRGASGRILACHLPSRTWFPGGAGVNDDLAALAEATVRHGKPQTAEIESSGQWHAALA
                     IPAIYLKGCVLIALIPFSRQDSLLAITHAGWLSGLLLGLVLPLGFWMILAHGILQPIQ
                     NILAGINRLRRVELDRPIPSTGDDELGRLAVGVNRMMTVAADLDLARVVQRCFTPEQL
                     PTVPGFTLHFWKSQRLGVGGAYFDCQTDAAGNLVLFFGSAVGQGIQAALIMAMVKSLS
                     FTHLQAGGSADDLLPMIGQALPGLGVSRPAMCLTIISLQSASCLFKAWTAGSPAVLFK
                     PYSNRSIVQFTSSSYPLGGRYRHLQEIAHQPFHPGDALLLMTHSVLTTAAATHPDDQL
                     LQLTERFARYDPGAAESAFQNLRREWSTHAGGRESGKEAFCLLARTPSRNAEAPGCSE
                     GDR"
     gene            3325..6075
                     /locus_tag="PKO06_02635"
     CDS             3325..6075
                     /locus_tag="PKO06_02635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68567.1"
                     /translation="MNTRRPSLPFSLLLALPLFFGWLMLEHARIERLRSETELGEEQL
                     FTSMQKCCYEIRPLSQIHRGLQSLRQHIRAHDYRYSAVSPWPRTFPEGYRVQAGIFSP
                     SGELSEPAWAPIPFKFVWKQFWQDFCRRRTTFAQLTRYQKAFGNWLGLDDVENDDLLQ
                     EVSTPSGIGFFYTLRGPEQAGLLLFVSDIPSPIERWKQRLQASHTPSLDLWIFDPRSD
                     AIYGAKPLTDREQRFLRLGQVQTGATRCEAGQRYATLRTEENLILLGRTPDRTDHRRG
                     LEQWVIAFLTIIPLLTWICAGDSFLLRLSIHTRLLLLFAASALLPYLLLREMSFRHQE
                     DIARQLESTIRQEQTARLHAAEDQLRHLPQRQNRVYQRLSREVRQRVAAGPEAMDAVL
                     RDARAQRLLDAYSLLDSRGQELCSHWNDQSIRDLAKLFAEEMLTRYRENRYPQPDDSL
                     RWSFFQVIQSPIIGFDTIADSRGICRPIKVSAAASALWYWDTFSDARASAPAFIGLVQ
                     GLANARLALFQRELPSGVFAFDATQHTWYPQAPRLPHVDELVGQAFVGRRTLATRQVS
                     PDGETLILLFPSAVEPNICFLIADDANSLRERISAIQRRYQLAGMFILIMTGFLASVL
                     ARTLLSPLEQLSRGVTTFESGGRVPVLTISTSDEIGVMTEAFNAMVQEAREVDAARKV
                     QESLIPLTPAQLPGIRSALRYLPLENLAGDYGDVIPRPDGTLLVAIGDVTGHGIGSAL
                     QTAMAKAVCYHACQTQATLPELFARLNAILHQGRSSRRFMTFCGGVLSPHTGIWHWIS
                     AGHGYPLIRYPDGRIEFLKSIGVPLGMQASRRWQVSEVKLTPGTMLLFYTDGLVEACN
                     VDQQPFDYEGLRTAFGQATGGPDEVAEQILRVFHRYTGNVPLTDDVTLLVLEWTGAPQ
                     PA"
     gene            complement(6017..>6656)
                     /locus_tag="PKO06_02640"
     CDS             complement(6017..>6656)
                     /locus_tag="PKO06_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68568.1"
                     /translation="FLPELLQELALTRGEPEAALIVLHAFHRRHPAARSVRRLTASFL
                     AMKERIRPALSLLESCLRDQPSVLADQFDAYRLSLLAGDLPRAEQFGRQVHALYRPLL
                     EACRVMAGELLGHDNGGFYEQLHALETPSLNLYNAMVQLKSRQGDRETVLELLGELFL
                     ARPTNGVIVRLLLKSGETLAEPVRVGLRAYLEALTPVGGRPSIREREESHRR"
BASE COUNT         1279 a         2114 c         1853 g         1410 t
ORIGIN      
        1 tccatgttcc acgacctgac gatcaactca ctcgacatcg ccgatctgat tctactgatg
       61 ctcgtgccga acatgaacca cctgaagacc acgcacgtct gcctgcaggt catggaaaac
      121 ctcaagtatc cgaaagagaa gatcaagctg ctgctcaatc gcgccaactg cgaacgcagc
      181 atgaatcgcg aagagctgga aaccggcctg aaacggaaga tcgacttcac gatgtccgat
      241 gatttccaga atgtcagcga actgatcgaa catcagaaga cggtctatga actgaacaac
      301 gagtctccgt accgagaaga catgaaccgc ctgatcgaag tgatcaccgg caaaaaagcc
      361 gatgcggcga aaaagggatt gaccgggtta ctgaagagct tcttcggcca gtagagaact
      421 ttttcgcgtt ttcttttacg aacgccattc agataatcgc acgacctgcg cccaccgcaa
      481 cccgggcgca ggtcgtgtgc tttttcaccc gagacgagat cacccccttg cgtcagcggt
      541 ttcgtctcac tggtgacagg ctcggctcac ctgcatgacc ccggcagtca cacgcaccgg
      601 accgacttct tcagtaccag ttctcattgg tctctgggta tttctggtgc tgattcccgg
      661 cttattcttc atctggatgt catctcagct gcacgaacag tggcgcttca acgagattca
      721 cactcgtcag aacgcgctcg aacgatcggt gcggcgactg cttcgtctcg cccacccttc
      781 gcaggacgcc ctggagaagc tggccgcgca ccccgatttc ccgagttcgc cgcctcccct
      841 ctcgctctct ccgcagggtc gagaggaaca cgccgttctt tcgctgcttc atcagttcct
      901 gaccgatggt cagtcgttga gccaggattc tcagctgcgt atctgtcatc acttcggctg
      961 ggtgttcaat ccgtatcagg ttcttgagca accgggagtt ccggtcgaga tcacccgggc
     1021 cggtcagagt gcctggctgg tatggtcagc cgcgactgcc acgcaaccgg tcagaatcgc
     1081 tctttctgcc gcgacgaatg cgttccctcg tttgtccgat ctctgcagcc agcagtttcg
     1141 cgacaccacg cgagaatggc tggtgatcga atcgacaggg ggccaggcaa ccggcgttcc
     1201 gtcgctgcgg cctgcgatcc cggatctgct ggccctggcc aagcgccagc agaacggtcg
     1261 ctggcaggcg gacgaacacg tcgggatctc tgacatggcc caggacggca ccacggtgtt
     1321 cctgcgagat cagctgtcac cgcgcgcacg tctttccttt cgcagccgcc tgcatgcggt
     1381 tctgctgacc ctgctggtca tcgtctcctg tgtctttccc gccctgtgtc gcacctatct
     1441 cgctctgccc ctcttctgga aactgctgat gctgctgggg tatctggtgg tgctgccgct
     1501 cgcagccagc ggctttctcg gacaaggatt ctttcactcg cgcgcgctca accacgcgga
     1561 actgctgcga acccgcgccc tcagcgacct cgaggtcttc gattccggat ttctgcactc
     1621 gattcagagt cttgaaagcc gcttccaggt gttgaaggaa gctctcgatc gtcggcggct
     1681 gtcttccgcg caggtgcaga agtttctcga tccatttttc caggcgcagg tcatcgatcg
     1741 ttttctcctg ctgagccatc ggggcgacat tctgctcgac cgctgctcat ctcgccggaa
     1801 ccactcgctg atgaatgaag tcggcatggc cttttgccgc gacctgttcg cccaacgacg
     1861 tctggggggg cgcaaagtcg aagaaccgaa acgctttacg ctgttcaacg acctgatcca
     1921 gaactccatc tggggaagcc actatctggt cgaaggtcgg ttacagcggt ttcttcacgg
     1981 gaccggcgtc ctctactcat actggacgct tctggcgccg cagaacccca ccttcggtct
     2041 catcactctc gatctctttc acgacggcct cgtcacacgg tatctgagaa ccacccccct
     2101 gcgcggggcc agtggtcgaa ttctggcgtg tcatctgccg tcgcgcacct ggtttcccgg
     2161 tggcgcgggc gtcaatgacg atcttgccgc acttgccgag gcgacggttc gccatggcaa
     2221 gccgcagacc gcagaaatcg agagttccgg acagtggcat gcggctctgg ctatccccgc
     2281 catctacctg aaaggatgcg tgctgattgc gctgattcca ttttcccgtc aggattccct
     2341 cctcgccatc acgcacgctg ggtggctgtc ggggctgctg ctggggctgg tactccctct
     2401 cggattctgg atgatcctgg cgcacggcat ccttcagccg attcagaata ttctcgcggg
     2461 catcaaccgc ctgcgtcggg tcgaactcga tcggccgatt cccagtaccg gcgatgatga
     2521 actggggcga ctcgccgtcg gcgtgaaccg catgatgacc gtcgccgcag atctcgacct
     2581 cgcccgcgtc gtgcagcggt gcttcactcc cgagcagctg ccgaccgtcc ccggctttac
     2641 gctgcacttc tggaaaagcc agcgtctcgg tgtcggcggc gcttacttcg attgtcagac
     2701 cgatgccgcc ggcaatctgg tgctcttctt cggctccgct gtcggacaag gaattcaggc
     2761 ggcactgatc atggccatgg tgaaatctct gtcgtttacg cacctgcagg cagggggttc
     2821 agccgatgat ctgcttccca tgatcggcca ggcgctcccc ggcctgggag tttcgcgacc
     2881 cgcaatgtgt ctcaccataa tcagcctgca gagcgcatca tgcctgttca aagcctggac
     2941 agccggcagt cccgcggtgt tgttcaaacc gtattcaaac cgttcgattg tacagttcac
     3001 ttcatcttca tatcctctcg gtgggcgata tcgacaccta caggaaatcg cccatcaacc
     3061 atttcaccca ggcgacgccc tgctgctcat gacccactcc gtgttaacca ccgcggcggc
     3121 aacccacccc gacgaccagc tgctccagct gaccgaacgg ttcgctcgat atgatccagg
     3181 ggctgccgaa agcgctttcc aaaacctccg ccgagaatgg tccacccacg ccggtggccg
     3241 tgaaagcggc aaagaggcct tctgtctgtt agccaggaca cccagccgga atgctgaagc
     3301 gcccggatgt tcggagggtg accgatgaac acccggcgcc cgtccctgcc attcagcctt
     3361 ctgctcgcgc tccctttgtt tttcggctgg ctgatgctgg aacatgcgcg aatcgaacgt
     3421 ctgcgatcag agacggagct gggcgaagaa cagctcttta ccagcatgca aaagtgctgt
     3481 tacgagatac gtccgttgtc gcagattcat cgcggcttac aatcgctgcg acagcacatt
     3541 cgagctcatg attatcggta ctccgccgtt tccccctggc cgcgaacttt tcctgaaggc
     3601 tatcgagtac aagcgggaat tttctctcct tccggcgaac tgtctgaacc ggcatgggcc
     3661 cccattccct tcaagttcgt ctggaagcag ttctggcagg acttctgccg acgccgcacg
     3721 acgttcgcgc agctgacccg ttatcagaaa gccttcggaa actggctcgg cctggatgat
     3781 gtcgaaaatg acgatctgct gcaggaagtc tcgacgccaa gcggcatcgg attcttctac
     3841 accctgcgcg gaccggagca agccggactt ctgctgttcg tctccgacat tccatcgcca
     3901 atcgaacgtt ggaaacaacg actgcaggca agtcacacac ccagcctgga tctctggatc
     3961 ttcgatccgc gaagcgatgc catctacggc gccaagcctc tgaccgatcg ggagcaacgg
     4021 ttcctgcgcc tcggccaggt gcagacgggc gcgacgcgct gcgaagcagg gcagcgctat
     4081 gccacgctga gaacggaaga gaatctgatc ctgctcggac ggacaccgga cagaaccgat
     4141 caccgtcgtg gcctggagca atgggtgatc gcgtttctca ccatcatacc tctcctcacc
     4201 tggatctgtg cgggagactc ctttcttctg cgtctcagca tccacactcg cctgttgctc
     4261 ctgtttgcgg cctcggctct cctgccctat ctgctgctgc gtgagatgag ttttcggcac
     4321 caggaagaca tcgcccggca actcgagagc accattcggc aggagcagac cgcccgactg
     4381 catgcggccg aagaccaact tcgccatctg ccgcagcggc aaaatcgcgt gtatcaacgg
     4441 ctttcacgag aggtgagaca acgggtggcc gcgggtccgg aggcgatgga cgcggtgctg
     4501 cgggacgcac gagcgcagcg tcttctcgat gcgtacagtc tgctcgacag ccgaggtcag
     4561 gaactctgca gtcactggaa cgatcagtcc attcgcgatc tggccaagct gtttgccgaa
     4621 gagatgctca cccggtaccg cgaaaatcgg tatccacaac cggacgattc tctgcgctgg
     4681 tctttctttc aggtcattca gagtccgatc atcggattcg acacgatcgc cgactcacgc
     4741 ggcatctgcc gaccaatcaa ggtcagcgcc gcggcttccg cattgtggta ctgggacacc
     4801 ttctcggatg cccgtgcctc agcccctgcc tttatcggtc tggtacaagg tctcgcaaac
     4861 gcacgtcttg ccttgtttca gcgtgaactg ccgtcaggag tcttcgcgtt cgatgccacg
     4921 cagcacacct ggtatccaca ggcacctcgt cttccccacg tcgacgagtt ggtcggtcag
     4981 gcattcgtcg gtcgaagaac cctcgccacc agacaggtct ctccagacgg cgagacgctg
     5041 atcctgttgt ttccaagcgc ggtcgagccg aacatctgtt ttctgatcgc ggacgatgcc
     5101 aactcgcttc gcgagcgcat ttccgcgatc cagcggcggt atcaactcgc cggcatgttc
     5161 attctcatca tgaccgggtt tctcgcgagt gtgctcgcac gaacgttgtt gtcgccactc
     5221 gagcaactct cgcgtggcgt gaccaccttc gaatccggag gcagagtacc agtgctgacg
     5281 atctcaactt ctgacgaaat aggcgtcatg acggaagcgt ttaacgccat ggtgcaggaa
     5341 gcccgcgaag tcgacgcggc ccgcaaggtg caggaaagtc tgattcccct cacgccggct
     5401 caactgccgg gaattcgcag cgcattacgc tatctgccgc tcgagaatct ggccggtgac
     5461 tatggcgatg tcatcccccg accggacgga acgctcctgg tcgcgatcgg cgatgtaacc
     5521 ggacacggca tcggttcggc tctgcagacg gcgatggcca aggccgtctg ttatcatgcc
     5581 tgccagactc aggcgacgct tcccgaattg tttgcacgtc tgaatgccat cctgcaccag
     5641 ggccggtcga gccgccgctt tatgacattc tgcggtgggg ttcttagccc gcacaccggt
     5701 atctggcatt ggatctcagc cggacacggg tatcccttga tccgctatcc ggatggacgc
     5761 atcgagtttc tcaagagcat cggcgttcct ctcggcatgc aggcgtcccg tcgctggcag
     5821 gtgtcggaag tgaaattgac gcctggaacg atgttgctct tctacaccga cggattggtg
     5881 gaagcctgca atgtcgacca gcaacccttc gattatgaag gacttcggac ggccttcggc
     5941 caggcgaccg gcggcccaga cgaggttgcg gagcagattc tccgcgtgtt ccaccggtac
     6001 acgggaaacg tgccgctcac cgacgatgtg actcttctcg ttctcgaatg gacgggcgcc
     6061 ccccaaccgg cgtgagcgct tcgagatagg ctcgcaaccc cacccgcacc ggttcagcaa
     6121 gcgtttcgcc cgatttcagc agcagacgca cgatgacgcc attggtcggg cgcgccagga
     6181 acagttcgcc gagaagctca agcacggtct cgcgatcgcc ctggcgtgat ttcagctgca
     6241 ccatggcatt gtacaagttt aacgatggtg tttcgagcgc gtgaagctgt tcatagaatc
     6301 caccgttgtc atgtccgagc aactctccgg ccatcacccg gcaggcttcc agcaatgggc
     6361 ggtacagggc atgcacctgt cggccgaact gttcggcccg cggcagatct cctgccagca
     6421 gggacaggcg gtaggcgtcg aactgatccg ccagcacgct gggctgatca cgcagacagg
     6481 attcaagcag ggacaaggcc gggcgaatcc gctctttcat ggcaagaaat gaagccgtca
     6541 ggcggcgaac agagcgtgct gccggatggc gacgatgaaa cgcatgcaga acgatcagtg
     6601 ccgcctccgg ttctccacgc gtcaacgcca gttcctgcag cagttccggg aggaac
//
LOCUS       DAORHD010000072         6614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11513_length_6614_cov_3.383443, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000072 DAORHD010000000
VERSION     DAORHD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11513_length_6614_cov_3.383443"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKO06_02645"
     CDS             <1..611
                     /locus_tag="PKO06_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014109904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNV68569.1"
                     /translation="MIAVDKADAAQFSVDEILHPTDWVLLNYLMDPRTGLGRFRNFRI
                     SNYNLMMDLIDCCKDMTIKQILDLPDVKERVELYREQQEKFIEQVKRCSSVYDKLIVL
                     DLRNEEVIHAGNRFMIYALYPETTISIHVMWGFQKQNTVFATGKSIVNRSSRFNIGEL
                     MLKYGGGGHQNAGTCQIAHDQSEKVLKELIEAITTADKAAGA"
     gene            1230..2792
                     /locus_tag="PKO06_02650"
     CDS             1230..2792
                     /locus_tag="PKO06_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNV68570.1"
                     /translation="MTVGTESAATETTAVATETVPATGAVTIIDITQLKADPFTLKKD
                     GFEEAGQKLGRKVDTLQERMSNHRAGWLKNEVIWGITWLKILLSVLLFSGLFFLELTL
                     RKLIRRLKRKVDAEEPSLKELSRQSDVWIDPLLVALQPPLSLFIWGYGGLTIMAFLFS
                     QVAKTDELYWIPQTAGQIAEMGGLIAFFWLLFRMITVLDVRLEKWADTTPSHWDNILV
                     PFVGRILRFILPMMGVIFSLPFFHFSPETTASIQCLVTITLIGSIAWILFQLILVVEK
                     GLLVPLGDTAREDLKSRKILTQVHILKKILFTIIGVVSLALMMMVFDSVRQLGTSILA
                     SAGVLGIIVGLAAQRTIGTLLAGIQIAMTQPIRIGDVVIVEGEWGNIEEISLTYVVVK
                     IWDLRRLVVPINYFIEKPFQNWTRTSEKVLGTVYFYVDYTFPVDALREPLKKIVSTSE
                     HWDKDVCGLIVTEAKERIMELRALVSAENSSKCWDLRCEVREKVIAWIQEHYPQSLPL
                     TRVMLTQAETAE"
     gene            complement(3277..5385)
                     /locus_tag="PKO06_02655"
     CDS             complement(3277..5385)
                     /locus_tag="PKO06_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68571.1"
                     /translation="MSEEVITHPTSEHRARALKLFLISCASLYFELLFLRWIPDSVHV
                     LSFFGNFTLLAIFLGLGIGLSQSQETTEDSIFRKIVRNLSLLSAAVLLFDVLRFSVRY
                     SGDYAFNVGPVNVEHRFNVYVVIFLFLSLAIYCFIPIGSLIRRYFATQAPLAAYTLNI
                     AGSLAGILLFTTLSYFGTPLWFWMLIGLLMLLPFTPEKGKYALIFLIMCGVIVAENRY
                     TEKVFNFQKFWSPYYGLQLRQVSKDHSYLTISNSFILSALNLSLPQETTREYYEVPYR
                     VKKEPENVLVLGAGMGNDVAVGLMNGAKHIDAVEIDPMIIELGKQYHPLKPFIDPRVT
                     IINDDARAFMRNSKQKYDLIVFGTLDSHGLFSLFSSIKMENYVYTVESFREAKNLLKD
                     GGLLYVNTGFMGVEFVNCRLYNCLKEVFGKDPLFFVYENAILMYLTGNIEQIDFSIFS
                     SKSFKRLTVNAEREKVESPENLILPTDDWPHLYLRDMMIPREYFFALLILFTVSAVFL
                     FTGLKRTTEFHGFYFLLGAGFMLLETKCITEMGLIFGSTWIINAVVISSILLVILVAN
                     LFLLKAAPADTTTYFYLALGGALIFGYFVPLDLLNQENFAFKLLLSALYIAIPVFFSS
                     LIFGINFRRESADASLCLAWNMLGSIFGGIIEYGSMIYGLKSLALFALCIYLLSYLLQ
                     FKTLIQKSDTIPQTQNGDTR"
     gene            complement(5555..>6614)
                     /gene="pgi"
                     /locus_tag="PKO06_02660"
     CDS             complement(5555..>6614)
                     /gene="pgi"
                     /locus_tag="PKO06_02660"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009195880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV68572.1"
                     /translation="IVASKTFTTQETMTNALTARQWLLDTLKDQKAVSRHFVALSTNK
                     TEVAKFGIDTENMFEFWDWVGGRYSSWSAIGLSICCSLGFRRFIELLEGAHAMDEHFR
                     TSPLEKNLPVILAMLGIWYNNFFGAESQAILPYDQYLHRFAAYFQQGDMESNGKYVDR
                     DGRPVDYQTGPIIWGEPGTNGQHAFYQLIHQGTKLIPCDFIGCVNPQHQMGDHHLKLL
                     ANFFAQTEALMKGRTEAEVKGDLEKAKVPAEEAKMLLPHKIFEGNRPTNSLLVKKLTP
                     RTLGMLTALYEMKIFVQGIVWRLNSFDQWGVELGKVLAKTILAEEEALVKGQKPDLSH
                     HDSSTRGLLTQFAAWREA"
BASE COUNT         1516 a         1691 c         1913 g         1494 t
ORIGIN      
        1 tgatgattgc ggtcgacaaa gccgacgcag cgcagttttc ggtcgatgag atcctgcacc
       61 cgaccgactg ggtgctgctg aactacctca tggatccgcg caccggactg ggacgtttcc
      121 gcaacttccg catctcgaac tacaacctga tgatggatct catcgactgc tgcaaggata
      181 tgacgatcaa gcagattctt gatctacccg atgtcaaaga gcgcgtcgag ttgtacagag
      241 aacagcagga aaagttcatc gaacaggtga agcgctgctc cagtgtctac gacaagctca
      301 tcgttctcga cctgcgcaac gaggaagtga tccacgctgg caatcgcttc atgatctacg
      361 cgctctatcc cgagacgacg atctcgattc acgtcatgtg gggctttcag aaacagaaca
      421 ccgtcttcgc taccggcaag tcgatcgtca accgatcgtc caggttcaat atcggtgaac
      481 tcatgctcaa atacggcggc ggaggccatc agaatgccgg cacctgccag atcgcccatg
      541 atcagtccga aaaggtcctg aaagagttga tcgaagcgat caccaccgcc gacaaggccg
      601 ccggcgcctg accgctcgtt tcgcgctccc catgcctcgc tccgctctca cggcgcgagg
      661 cgtttctctt catagcgggg gaagtttctt gatggaaacc ggaaagaacg aactgaattt
      721 ctcacgaaga acgcttctcc tggtcatcga agaaccagac ggtgctgtgt ttggcccagg
      781 gtcatgcagt tctctcccag ttttttgtcg gcatgagcgc actccctgcc gcaacggcga
      841 atcgttgagt catggcgacg aaaaacagct tctcgcgaga tgtaattgac cctgggtttt
      901 gtccgtcacg ggttgtattg atttcgcgac atgggtaaaa tgagcggaaa cacacgtgcg
      961 gctgtggtgt catgcacggg gccatttttt cctcgtctga ggcatgacct gggccaggca
     1021 gccgtctgca gacaaggagt tccgtcatga gtcactttgt tgcgttccgt tctttctttt
     1081 gttcctcggc aactctgaga tgtgtgattt gtctctggtt gtctgtattc gcttcatctg
     1141 tatggtgcgt tgagagcagt ttacgttctt cggcaacagg tactgtcgcg gcagaagtcg
     1201 cgtccgttcc cgctggcagc gtggcgagcg tgaccgtagg tacggaaagt gcggcaaccg
     1261 agactacggc ggtggcgacc gagacggtgc cggccacagg tgcggtcacg atcatcgata
     1321 ttacgcagtt gaaagccgac ccatttacgt tgaaaaagga tgggtttgaa gaggccggcc
     1381 agaagctggg gcggaaggtc gataccttgc aggaacgtat gagcaaccat cgtgcggggt
     1441 ggctgaagaa tgaagtcatt tggggtatca cctggctaaa gattctgcta tcggttctcc
     1501 ttttcagcgg gttgttcttt ctcgaactga ccctcagaaa gctgatacgg cgcttgaaga
     1561 ggaaggtcga tgcggaggaa ccctctctca aggaactgag tcggcagtcg gacgtctgga
     1621 tcgacccctt gctggtggcg cttcagccgc cgctctcgct gtttatctgg ggatacggtg
     1681 gtctgacgat catggcgttc ctgttcagtc aggtcgccaa aaccgacgaa ttatactgga
     1741 ttccgcagac ggccggtcag attgcggaaa tgggtggtct gatcgcgttc ttctggcttc
     1801 tgttccgcat gatcaccgtg ctggatgtaa gactggagaa gtgggccgat accaccccca
     1861 gccactggga caacattctg gtgccgtttg tcgggcgcat tctgcggttc attctaccga
     1921 tgatgggcgt cattttttcc cttccgtttt ttcacttttc accggagacc accgcgtcga
     1981 ttcaatgcct ggtgacgatc accctgatcg gttcgatcgc gtggattctc tttcagctca
     2041 ttctcgtcgt tgaaaaaggt ctgttggtcc cgctgggaga tacggcgcgc gaggatctga
     2101 agtcgcggaa gattctgact caggtgcaca ttctcaagaa gattctgttc accattattg
     2161 gagtcgtctc tctggcattg atgatgatgg tcttcgacag cgtacgacag ctgggaacca
     2221 gtattcttgc atccgccggc gttctcggta tcatcgttgg tctggcggcg cagcgcacga
     2281 tcggtacgct tctcgccggg attcaaatcg ccatgactca gccgattcgg atcggcgacg
     2341 tcgtgatcgt cgaaggcgag tggggcaata tcgaagagat ctcattgacc tatgtcgtgg
     2401 tcaagatctg ggatctccgc cgtctggtgg tgccgatcaa ctacttcatc gagaagccat
     2461 ttcaaaactg gactcgaacg tcagaaaagg tgctgggcac agtctacttc tatgtcgatt
     2521 acacttttcc ggttgatgcg cttcgcgagc cgctcaaaaa gattgtttcc acatctgaac
     2581 actgggacaa agacgtttgc ggactgattg tgaccgaagc aaaggagcgg atcatggaac
     2641 tgcgcgccct ggtgagcgcg gaaaactcgt cgaagtgctg ggatctgcgc tgtgaagtgc
     2701 gggaaaaagt cattgcctgg atacaggaac actatccaca gtcactacct ctgacccgtg
     2761 tgatgctgac gcaggcagaa acagcggagt gagtgagaga ggcttgcaga tccgcggcga
     2821 gatgtttggg gcggttgaac ggctaaaaga agtccggaag tggagagtca tcctggtgga
     2881 tggcgagtcc ttcgatttcc agccgccaac gggagcggca aaccaggcgc ggcactccgt
     2941 tcccacggcg gcgtggtgtt tgtcgatgtt ctgcacgaag atttaggaac gaagagtgtt
     3001 cccttgagcc gtcatctcgt gtcgctgcag cgactgttcg agctgatcga atccgcgcgc
     3061 gtctgggcag ccgattgccg gtgttgagtt ggcgttcctc cagcatccgc tgatagctgc
     3121 gacacagcag attgaacccc ttggcaaacg agtgcctgcc cgggtcgaca gtgtcagacc
     3181 atgttcgacc gccccacgac atgtcgagaa ggaaaactcc tgttgatgtt cgttcgacgg
     3241 ttttgtcgat ggttcagcca tggatgcctc tgccgctcaa cgagtatcgc cgttctgtgt
     3301 ctgcgggatg gtatcagatt tctgaatcag cgttttgaat tgcagcagat aggacaacag
     3361 atagatgcag agagcgaaga gtgccagaga cttcaggccg tagatcatcg atccatactc
     3421 gataatgccg ccgaagatcg aaccgagcat gttccaggcc aggcagagcg aggcgtcggc
     3481 cgattcgcgc cgaaaattga tgccgaaaat cagcgacgaa aagaacacgg ggatggcgat
     3541 atacagcgcg gagagcagta gtttgaaagc gaagttctct tgattgagaa gatcgagtgg
     3601 cacgaaatat ccgaagatga gagcgccgcc cagggcgaga tagaaatacg tcgtcgtgtc
     3661 ggcaggagcc gctttcagca gaaacaggtt ggcgacaagg atgacgagga gaatactcga
     3721 tatgaccaca gcgttgatga tccaggttga gccgaagatc aggcccatct ccgtgataca
     3781 cttggtttcg aggagcatga atccggcgcc gagcagaaaa tagaaaccgt gaaattcggt
     3841 ggtgcgcttg agccccgtga agagaaacac ggcagagacg gtgaagagaa tcagaagcgc
     3901 gaagaaatac tcacggggaa tcatcatgtc tctgagatag agatgcggcc aatcgtcggt
     3961 cgggagaatc agattctctg gagactcgac tttctctcgt tcggcgttca ccgtcaggcg
     4021 tttgaaagat ttcgaagaaa agatcgagaa atcgatctgc tcgatgttgc cggtcagata
     4081 catcagaatg gcattctcgt agacgaaaaa gagagggtcc ttgccgaaga cttctttcag
     4141 acagttgtag agacgacagt tcacgaactc gacacccatg aagccggtat tgacatacag
     4201 caggccgccg tctttcaaca ggtttttcgc ttcgcgaaag ctttcaacgg tatagacgta
     4261 gttttccatc tttatcgacg aaaagagtga aaacaatccg tgggaatcca gcgttccgaa
     4321 gacgatgaga tcgtacttct gtttactgtt tctcatgaag gcgcgggcat catcgttgat
     4381 gatcgttacg cgagggtcga taaagggctt gagcgggtga tactgcttgc cgagttcgat
     4441 gatcatgggg tcgatctcga cggcgtcgat gtgtttcgct ccgttcatga gcccgacggc
     4501 aacgtcattt cccatgccgg ctcccaggac aaggacgttc tccggttcct tctttacccg
     4561 atagggaacc tcatagtact cgcgagtggt ttcctgcggc agggaaagat tgagagctga
     4621 caggatgaag ctgttgctga tcgtcagata gctgtgatcc ttgctgactt gtcgcagttg
     4681 cagaccatag tagggcgacc agaacttctg aaagttgaac accttctcag tgtaccggtt
     4741 ctcggcaacg atcaccccgc acatgatcag aaagatgagc gcatactttc ctttctcggg
     4801 ggtgaatggc agcagcatga gtagaccgat gagcatccag aaccagagtg gagtgccgaa
     4861 gtaggaaaga gtggtgaaca gcagaatgcc agccagactg ccggcgatgt tcagggtata
     4921 ggccgcgagt ggtgcttgcg ttgcaaagta gcggcgaatc aggctgccga tcggaatgaa
     4981 acagtagatg gccagcgaga ggaacagaaa aatgaccaca tagacgttga aacgatgctc
     5041 gacattgacc ggaccgacat tgaacgcgta atcgccggaa tacctgacgc tgaacctgag
     5101 aacatcaaac agcagaactg ccgctgagag cagactcagg ttgcgaacga tctttcggaa
     5161 aatcgaatct tcggtggttt cctgcgattg gctgagtccg atgccgagac cgaggaaaat
     5221 cgccagcagc gtgaagttgc caaagaagga aagcacatgt accgagtccg gaatccaccg
     5281 caaaaacaac agctcaaaat acagcgacgc acaactgatg agaaacagct tcaatgcccg
     5341 tgcgcggtgt tctgacgtcg gatgagtgat gacttcttcg ctcaaggtgc gtctcctcgg
     5401 aaagacggta tggtcattga atcagacgat catgcggaat cgtgatgaga taccgaagaa
     5461 aagtccattc gtggcatgag atgaaacgat cgcatcaggc acacctcgac aaccgctgca
     5521 cgaacggctc ttcgtgtgtc tcgtgtctgg tgtgtcacgc ttcgcgccag gcggcgaact
     5581 gggtgagcag gccgcgggtc gaagagtcgt ggtgcgacag gtcaggcttc tggcctttca
     5641 ccagcgcttc ctcttcggcg agaatcgttt tggcgagcac tttgccgagc tcgacgcccc
     5701 attgatcgaa gctgttgagg cgccagacaa tgccctgaac gaagatcttc atctcataca
     5761 gagcggtcag catgccgagc gtgcgcggcg tgagtttttt cacaagaagc gaattcgttg
     5821 gtcggttgcc ctcaaagatc ttgtgcggaa gcagcatctt cgcttcttca gccggtactt
     5881 tcgctttctc gagatcgcct ttcacctcgg cttcagtgcg ccccttcatc agcgcttcgg
     5941 tctgcgcgaa gaagttcgcc agcagtttca gatgatgatc gcccatctgg tgctgcgggt
     6001 tgacgcagcc gatgaaatca cacggaatca gtttggtacc ctgatgaatc aactggtaga
     6061 acgcgtgctg accgttggtg cccggctcac cccagatgat cggcccggtc tgatagtcga
     6121 ccggccggcc gtcgcgatcg acgtacttgc cgttgctttc catgtcgccc tgctgaaagt
     6181 aggcggcgaa ccgatgcaga tactggtcat acggcagaat cgcctgcgat tcggctccga
     6241 agaagttgtt gtaccagatg ccgagcatcg caagaatgac tggcagattc ttttcgaggg
     6301 gagaggttct gaaatgctcg tccatggcat gcgcgccttc gagcagttcg atgaatcgcc
     6361 tgaacccgag ggaacagcag atcgagagcc cgatcgccga ccagctcgaa taccggccgc
     6421 cgacccagtc ccagaactcg aacatgttct cggtgtcgat gccgaacttc gcaacctccg
     6481 tcttgttcgt cgacagggcg acgaaatggc ggctcacggc cttctgatcc ttcagagtgt
     6541 cgagcagcca ctgccgcgcg gtcagggcgt tggtcatcgt ctcctgcgtg gtgaaggttt
     6601 tcgacgcgac gatg
//
LOCUS       DAORHD010000073         6609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11526_length_6609_cov_4.717730, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000073 DAORHD010000000
VERSION     DAORHD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11526_length_6609_cov_4.717730"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKO06_02665"
     CDS             complement(<1..246)
                     /locus_tag="PKO06_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68573.1"
                     /translation="MTISPTATQAPSTGVPAAFPQPGSTSRNEAEVVWLVAPERAWSI
                     LFWALLLYLASIGGRFAWEERIQFFYEAGLLYLIGVGS"
     gene            complement(301..777)
                     /locus_tag="PKO06_02670"
     CDS             complement(301..777)
                     /locus_tag="PKO06_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68574.1"
                     /translation="MALTTQIFTFPAGGLWWVFGFITFVATAEPLQIYRKHRDQPAVR
                     KRCLIRLALRLLLVVLLIPPFTWAVTEGRWGAIIRLRDRELIGTTYFGRFLGKIDLDG
                     VSLRLEHRAHSRGSTWTLVGAKKFEHLRIDGFVDRSGRSLVDVVRVDFHVPTVEMR"
     gene            complement(783..2684)
                     /locus_tag="PKO06_02675"
     CDS             complement(783..2684)
                     /locus_tag="PKO06_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68575.1"
                     /translation="MTENPSSYRRYLSRLPVPGVLPLLIFALFLTLPTLAWTKKGKKP
                     AKEKGSTSEKSKPSKAELSAVKAAAKARKSLAKARTLRDKGKIEMALVEFQSAIDLDP
                     SYAEAYLEMGGLYFDSNLPDPAAQALELGIPLARQQGIDAVLVAQAYCQLAESYRRIG
                     RLNEALDQAKSASEVAPQDALPWKISGATHLDSDNEPEALKSLQEAVRLDPQFVEAWE
                     LLGQVALRRKDEALAQRVYEGLYRCDTFKADLYKTDMNQSGLKAAAPPKTKPVPPSAH
                     MDDPYAIGPATAPASFAAPATAGEPKKATATATVATQTIPRPAPPTPPVTPTRPVPAK
                     VVRPAGQKTFTGDEPPLLSLPTRPTSPVRPTERTSATTENASRSAAIKPTTAFRSAKP
                     LDPSDTLLTEVIPSGATTTMPILDENGEPAASPPTESEGSAGDTMQPPSETSDNDDPY
                     APPHASPPADGEAATSEAGDTTPNDSTAADPTLPDGEGDGSTTSSDEPALPMLPEDQV
                     PAPAAAVPGANTDDELIVVASTGSDEIPEDRIFALFSNLSSPDADIADGARKRLVGFG
                     AAAAPHLMQRLSDSNPLVRLRVMQTLGDLGGNAQVAVDQIRKLSTSDPDPKVRQEAQK
                     LVQSLTSSR"
     gene            2881..3276
                     /locus_tag="PKO06_02680"
     CDS             2881..3276
                     /locus_tag="PKO06_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNV68576.1"
                     /translation="MTEKAEQPGVEVIVTAAAEVYRILGPGLPEKVYVECLTRELSLR
                     GVHVQSQVPVRLTYKGVRLDHETSLPLLVEGTVAVWVEALAELSSLHEARLRTCMKFG
                     GWKQGVLLSFHVTRFEDGVRRLTATESDS"
     gene            3417..4775
                     /locus_tag="PKO06_02685"
     CDS             3417..4775
                     /locus_tag="PKO06_02685"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HNV68577.1"
                     /translation="MNSQLVVRRYLSGFVLVILLFLWLVPLPAATDAETRTPPLQQGT
                     LVLIGGALAAPGDEILNALKRSVTGLCRREGRHVPRVAFVPSSKQSLQQAHDDFEQDD
                     PDEPQRYPSYRTWFERAGFEPLLVPITQETVFDRRKAAEDARLADLIRSCQVVFLMGG
                     DQGKHAMCLLRRDGSPSAAMMAIREVYERGGAVVGTSAGTHAMANPMFGWGESGLTIL
                     FSRLEPFTLSDLTPKLPVEPKREGNCASVPGLGLLPSGMLTDTHFDARGRLGRLLVGL
                     RDLGLRWGIGVDENTALFLQNGRGVVHGERGVFIVDTASATFSLPGEPFQARQVRVSL
                     LTQGDIWQTTTGTIETSKPVVQNVAGAARFSSDVFSATKTSSGDRARPYETTLVMRSL
                     GASADREVVARAWPPTASIWVRFRKTTDTRVWGSALNSTIAHLVLDVDRIPFFKEHTS
                     EP"
     gene            complement(4908..>6609)
                     /locus_tag="PKO06_02690"
     CDS             complement(4908..>6609)
                     /locus_tag="PKO06_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNV68578.1"
                     /translation="TFLNLMTTTFGRVLGGVHGRLSEFTRALSQEAFHWHFQDFAAHL
                     LFSTRYRVGTPFLGSNNAQQRTVTLGATAPMLATQSVGLPQGMTATTRLIRVEVPAGR
                     RSRRVRVVTEKTDWINLWLYHLPGGQVQRAPRPIADLYAYHPDQVLDVAPGDLLALLA
                     CNFDWNQLRNPAGLQTFRFSVLDESPILTLTATPENGDQAKLLLRAEVTRIPAEVRRL
                     RWDWQLGDGRRREDFSNHRDEASLVIERRAHFPQVAEQSLEVRLFDVTGTASTELALA
                     SMTWRPIDLTLTADPNPVETNAPCEVQAVAKNAPRGVRYQFFFSDTPTLVPMKADRIR
                     HTFPAPGTYSASVHLLASGSAQTVLAIASTTVSVVEATLAEPRLSAPFPLGYVELQRG
                     EYSEGNQDFAEGMAFTYQILERHETVSGEFEIRDLRGPTQKLGAGRGRATFTARCRFI
                     ETKADDPWLKQWGSPLIDRESRSEKTLPFVFDRQNNSYSTSAEGRKQGYPPQVIGRLG
                     RLLVIIIMEPRCMGENSPSGQASFLPSVAIDDMAQSDAFTLWQAISDRFRRRAPATQR
                     "
BASE COUNT         1265 a         1873 c         2078 g         1393 t
ORIGIN      
        1 agaaccgacc ccgatcagat acagcagacc ggcttcgtag aagaactgga tccgttcttc
       61 ccaggcgaac ctgccgccaa tcgacgcgag gtacaacagc agggcccaga agagaatcga
      121 ccaggctcgc tccggggcga ccagccagac aacttctgct tcatttctcg aagttgaacc
      181 gggttggggg aaggccgcgg gcacacctgt tgaaggggcc tgagtcgctg tggggctgat
      241 ggtcatgagt cttgccgtgt tctcctcgtg tcctgtggtc attctacacc cgatgaattc
      301 ctatcgcatc tcgaccgtcg gcacgtgaaa gtccacgcgc acaacgtcga ccaacgatcg
      361 cccggatcgg tcgacgaagc cgtcgattcg cagatgctcg aactttttcg ccccgaccag
      421 cgtccaggtc gacccgcggg aatgagctcg atgctcgagc cgcagtgaga ctccatccag
      481 atcgatcttg ccgagaaaac gaccgaaata cgtggtgccg atcagctctc gatcgcgcag
      541 gcggatgatg gctccccatc gcccttcggt gaccgcccag gtaaacggag ggatgagcag
      601 gacaaccagc agcaggcgca atgcgagtcg tatcagacac cgctttcgca cggcgggttg
      661 gtcgcgatgt tttcgataga tttgcagcgg ctcggcggtc gcgacgaagg tgatgaagcc
      721 aaagacccac cagagaccac cggcgggaaa cgtgaagatc tgagtcgtca gagccacgga
      781 aatcagcgcg aagacgtcag tgattgtacg agtttctggg cttcttgacg cactttggga
      841 tctggatctg aagtgctgag tttgcgaatc tgatcgacgg ccacctgcgc atttccgccg
      901 aggtcgccca gcgtctgcat cacccgtagt cggacaagcg gattgctgtc gctcaaccgc
      961 tgcatcaaat gtggggccgc agcggctccg aacccgacca gtcgcttgcg cgcaccgtca
     1021 gcgatgtcgg cgtccggact cgatagattg ctgaacaggg cgaaaatacg gtcttcggga
     1081 atctcatcgc ttccggtcga agcgacgaca atcagttcgt cgtcggtgtt cgcccccgga
     1141 accgcggcgg ctggtgccgg gacctgatcc tcgggcagca tcggcagtgc tggctcgtcg
     1201 gacgaggttg tcgatccgtc gccttctcca tcaggcaatg ttgggtcagc agccgtcgaa
     1261 tcgttcggag tggtgtcgcc ggcttcggat gtggcagctt cgccgtcagc cggaggggaa
     1321 gcgtgaggtg gagcgtacgg atcgtcgttg tcgctggttt cagaaggcgg ctgcattgtg
     1381 tcgccagccg aaccctcgct ctctgttggt ggagaagccg ccggctcgcc gttttcgtcg
     1441 aggatcggca tcgtcgtggt ggcccccgaa ggaatgactt cagtcagcag ggtgtcggaa
     1501 gggtcgaggg gtttcgccga acgaaacgcc gtggtcggtt tgatcgccgc tgaacgtgac
     1561 gcgttctctg tcgttgccga tgttcgctct gtcggtcgca ccggagacgt cgggcgagtg
     1621 ggtaacgaca gcagtggtgg ctcatcacct gtaaaggtct tctgaccagc tggacgaaca
     1681 actttggctg gtactggccg ggtcggagta accgggggtg tcggtggtgc cggccgagga
     1741 atcgtctgcg tcgcaacagt cgcggttgca gtcgctttct ttggttcgcc tgcagtggcg
     1801 ggtgcagcga aagacgctgg tgccgttgct ggcccgatgg catacggatc atccatgtgc
     1861 gctgatggtg gcacgggttt ggtcttcggc ggggcggctg ccttgaggcc gctctggttc
     1921 atgtcggttt tgtacaagtc ggctttgaac gtatcacagc gatacagacc ttcgtagact
     1981 ctctgagcta gcgcttcgtc tttgcggcgc agtgccactt gtcccagaag ttcccaggcc
     2041 tcgacgaact gcggatccag gcgtaccgct tcctgaagag acttcaaggc ttcgggctcg
     2101 ttgtcactgt ccaggtgtgt ggcacccgag atcttccagg gaagggcgtc ctggggcgcg
     2161 acttctgagg cactcttcgc ctgatccagc gcttcgttca accgcccgat gcggcggtag
     2221 ctctcggcga gctgacagta ggcttgcgcg acgaggaccg catcgattcc ctgctgacgg
     2281 gcaaggggaa tgccgagttc gagcgcctga gccgcggggt cgggcagatt tgagtcgaag
     2341 tacaggccac ccatttcgag atacgcttcc gcatacgaag gatcgagatc gatggcactc
     2401 tgaaactcaa cgagagccat ttcgattttg cctttgtcac gcagagttct tgcctttgcc
     2461 agacttttgc gggcctttgc cgcggccttg acggcggata actcggcctt cgagggcttg
     2521 ctcttttcgc tggtgctccc cttttccttg gctggcttct tccctttctt tgtccacgcc
     2581 agagtcggaa gcgtgaggaa caacgcgaag atcagaagcg gaagcacgcc gggtaccggc
     2641 agtcggctca gataacgacg ataagaagag gggttctcgg tcatgaaata ctcctctgac
     2701 ggcgattgaa cacccgaagt gaggacgaac caaacgtttg tcgcatgata ccatgtcagc
     2761 ggtttccggt gcagccatct gctcctgacc agacgatgaa cttttcgttt tcgaagcggt
     2821 gaaaaacgag tacaatacgc ggagagtgag agaccgccga accgcaagga gtcgaagggc
     2881 atgacggaaa aagcggaaca accaggtgtc gaggtcatcg tgaccgccgc cgccgaagtg
     2941 tatcggattc tcggcccggg tctgcccgaa aaagtttacg tcgagtgcct gacccgcgaa
     3001 ctctcgttgc gtggtgttca tgttcagagc caggttccgg ttcgattgac ctacaagggg
     3061 gtcaggctcg accacgaaac gtctcttccc ctgctggtcg agggaacggt ggcagtatgg
     3121 gtcgaagcgc ttgccgagtt atcctctctg cacgaagcac gtttgcgcac ctgcatgaag
     3181 ttcggcggct ggaaacaggg ggttctgctg agtttccacg tcacgcgttt cgaagacgga
     3241 gtacgtcgtc tcacggccac agaatctgac tcgtagaacg ccgcttcggg atcgactgac
     3301 caacgggcac cagcctcaga acctcgcatc aacggtggca acccgcgtca ggggttatca
     3361 atcccgctct ggtatactgc gctcgattat cattttcaga ggggaagggc tttgtcatga
     3421 atagccagtt ggttgttcgg cgatatctgt ctggtttcgt gttggtcatt ctgctgtttc
     3481 tctggctcgt gcctttacct gcggcaactg atgctgaaac ccgtacaccc ccgctgcagc
     3541 agggaacgct ggtgctgatt ggtggcgcgt tagccgcccc tggagatgag attctgaacg
     3601 ctctcaagcg ctcggtgacc gggctctgcc gccgggaagg gcggcatgtg ccgcgcgtgg
     3661 catttgttcc atcatcgaaa cagagcctgc aacaggcaca tgatgacttt gaacaggacg
     3721 accctgacga acctcagcgc tacccgagtt atcggacctg gttcgaacgt gccgggttcg
     3781 agccgttgct ggtacccatc actcaggaaa cggtcttcga cagacgcaag gcggcggaag
     3841 atgcacgcct tgctgatctg atccggtcgt gtcaggtcgt gtttctcatg ggcggcgacc
     3901 aggggaagca cgcgatgtgt ctgcttcggc gagatggttc gccaagcgcc gccatgatgg
     3961 cgattcgtga agtgtacgaa cgaggtgggg cggttgtcgg caccagcgcc ggaacgcatg
     4021 ccatggccaa cccgatgttt gggtggggcg aaagtggact gaccattctg ttctcgcggc
     4081 tcgagccctt cacgttgagt gaccttacgc cgaagttacc ggtcgagccg aaacgcgaag
     4141 gcaactgcgc ctcggtgccc ggtcttgggc tgctcccttc ggggatgctg accgacactc
     4201 actttgatgc ccgcggacgc ctgggtcgac tcctggttgg ccttcgcgac ctcgggcttc
     4261 gctggggcat cggtgttgat gagaataccg cgctgtttct tcagaacggt cggggtgtcg
     4321 tgcatggaga acgcggagtc ttcattgtcg atacggcctc ggcgactttt tcgttgcccg
     4381 gcgaaccttt tcaggcgcgt caggttcggg tgtctttgct gacgcaggga gatatctggc
     4441 agacgacgac cggtacgatt gagacgagca agccagtcgt gcaaaacgtc gctggtgcgg
     4501 cgcggttttc gtcagatgtt ttcagtgcga cgaaaacctc gagtggcgat cgtgcccgtc
     4561 cttatgaaac caccctggtc atgcgctcac ttggcgcgag tgccgatcgc gaagtggttg
     4621 cccgtgcctg gccgccgacg gcctcgatct gggttcgatt ccgtaaaacg accgatacgc
     4681 gcgtgtgggg ttcagctctg aatagtacta ttgcccacct ggttcttgat gtcgatcgga
     4741 tcccgttttt caaagaacac acgtcagagc catgaaggct gtccggaagc gcgagacgcg
     4801 agaatctgga acgacgtcga aaagataaaa ccctcgcgga tgcctgccgg cgttgtctgc
     4861 ttcgcagtcg cagacgatgc ctgccgactc ggtcgtgaaa cccctgtcta gcgttgtgtg
     4921 gcgggagccc ggcgtcgaaa tcggtcgctg atggcttgcc acagagtgaa ggcatcgctt
     4981 tgggccatgt cgtcgatggc gacgctcgga agaaaactcg cctggcctga cgggctgttc
     5041 tcgcccatgc atcgcggttc catgatgatg atgaccagca gccggccgag tcgcccaatg
     5101 acctgcggcg ggtatccttg cttgcgccct tcggctgacg ttgagtacga gttgttctgt
     5161 cgatcgaata cgaatggaag cgtcttttcg ctacgcgatt cgcgatcgat caggggcgag
     5221 ccccattgtt tcaaccacgg atcatcggct ttcgtttcga tgaatcgaca ccgcgccgta
     5281 aacgtcgctc ggcctcgacc tgcgcccagc ttctgcgtcg gcccgcgcag atcgcgaatc
     5341 tcgaactcac ccgaaaccgt ctcgtggcgc tcgagaatct ggtaggtgaa tgccatacct
     5401 tcggcaaagt cctggttgcc ttcggaatac tcgccccgct gaagctcgac atagccgagt
     5461 ggaaacggtg ccgacaagcg cggttccgcg agcgtcgcct caacgacgct gaccgtcgtg
     5521 gaggcgatgg ccagcaccgt ctgagccgaa cccgacgcaa gcaggtgaac gctcgcggaa
     5581 taggtgccgg gtgcggggaa tgtgtgacga atgcggtcag ccttcatcgg aaccagcgtc
     5641 ggggtgtcgc tgaagaaaaa ctgatatcga acgccgcgcg gggcgttctt cgctacggcc
     5701 tgcacttcgc agggtgcgtt ggtttcgacc gggttcgggt cagccgtcag agtgaggtcg
     5761 atcggccgcc aggtcatgct tgccagtgcg agttccgtcg aagcggttcc ggtgacatcg
     5821 aacaaccgca cttcaagaga ttgttcggca acctgtggaa aatgggcccg gcgttcgatg
     5881 accagagatg cttcatcgcg atgattcgag aagtcttcgc gtcgccgtcc gtcacccaac
     5941 tgccaatccc agcggagacg acgtacttcc gcgggaatgc gggttacctc ggcgcgaaga
     6001 agcagtttcg cctggtcacc gttctctgga gttgccgtca gcgtcagaat cggagattca
     6061 tcgagaaccg aaaaacgaaa cgtttgcaac ccggcggggt tgcgaagctg gttccagtcg
     6121 aagttgcagg ccagcagagc gagaaggtca ccgggagcga cgtcgagaac ctgatcggga
     6181 tgatacgcat agaggtcggc gattggccgt ggagcacgct gaacctgccc acccggcaga
     6241 tgatagagcc agaggttgat ccagtctgtc ttttccgtga ccacccgtac ccgtcgcgat
     6301 ctgcgtccgg cagggacttc taccctgatc agccgtgtcg ttgcggtcat gccctgaggc
     6361 agaccgaccg actgtgttgc cagcatcggc gccgtcgcgc ccagggtgac cgttcgctgc
     6421 tgcgcgttgt tcgatccgag aaacggcgtg ccgactcgat agcgcgtcga gaacagcaga
     6481 tgggccgcaa aatcctgaaa gtgccagtga aaggcctcct gcgatagagc acgggtgaat
     6541 tcgctgagtc gcccgtgcac gccgccgaga acacgcccga acgtcgtggt catgagattc
     6601 agaaatgtc
//
LOCUS       DAORHD010000074         6443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11982_length_6443_cov_3.985911, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000074 DAORHD010000000
VERSION     DAORHD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11982_length_6443_cov_3.985911"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKO06_02695"
     CDS             <1..485
                     /locus_tag="PKO06_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68579.1"
                     /translation="EAKVRNRLNPERIDVVGFFLAEAEEAARLAALTAIRALRAEYPE
                     KTDMPEVQKYHFLGANCVSSGIQNLTRSIRPAYHHFAKQLVRKDVDEFQEWNAALACQ
                     WIKSWNELTLLAFDGLRGGRIDWKKLSEVSAFHKNLRSHNQMSLPWRAEPIIKWVAGK
                     "
     gene            496..1296
                     /locus_tag="PKO06_02700"
     CDS             496..1296
                     /locus_tag="PKO06_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68580.1"
                     /translation="MSFSPAEAQSAFRRLIRQPFFTYLLLILGLATAGIYSTIVRDDF
                     QIVKGAEMALLAITRFIILGQLIEFFRGLMILGLGTPLVMGLSSPLFYYSRQLRLEPF
                     LETFGIGLLPGLGLFFLGWKGLQLHRRNQQAGPGAEPETAADGFCARWLPTVEQACEW
                     SIWIMIATLMSGEWSGIGVLAVGFAGLTLAFSLVILVGAKTSIGRRGIGISLVIAGLV
                     TILTSIVLVTTQGNRCTEAVLGIPPGILLVIIGLALWLGASSRHARVS"
     gene            1408..1704
                     /locus_tag="PKO06_02705"
     CDS             1408..1704
                     /locus_tag="PKO06_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68581.1"
                     /translation="MSYQTEQPLPAPQHHATTLRAICLPCPPMYQGFSSWQELARLVW
                     ECDLSSYSLLAAFETWKRSDGSKVGLLKLPTRPEYLRRRHEVTRDSARDGASGE"
     gene            complement(1664..4540)
                     /locus_tag="PKO06_02710"
     CDS             complement(1664..4540)
                     /locus_tag="PKO06_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68582.1"
                     /translation="MSLRSILRAHLPLLRWLGQVLIFFAIPLLIVTAGFGRMWERQER
                     ERHDQAFTRLDRHLALIRKRSNTIVYLGAQFDALKQFIRRSPFRERTERMRKAIATFH
                     ARFPGMLRIIMFDREGRRLCPELDPSVPDSIVRRLSEVLCGEDETRLEAAKRYRPILS
                     AFLGPGVEPEQLAQGAKLKPVDANPGGEWRWLSFATGKWGGFCIQVRMVPDWDQAPLN
                     DQIRLLRQLRPKRQVDFGLVDLRRHAYAPPDVAVALRQYGVTTRQHALLPASLVTLQQ
                     IDAGVFLWASIPRRLLGDFDSRRLRLIFWVTAVFSVLCLVSARVMLGQSTFVLSIRQR
                     LLLLFAFAGGVPLVLIAFAQWDALQDLERRRSREATEAMERHLQALDNRYANLRADIE
                     LKLGQMLRPDRFADLEARKNWREVLEEVRRSFSPREIYVFNPNGKVLLRYQENANPPW
                     MEKNIREEGSTRLLGSLIRTLCSRLQPVEESVSGEMAVSIAEQFTGGELPVGEMVSNL
                     GKVFEITVFRDQHWIFLQPMRSAGGRIERVVSVSWKRDLLERLYLQRAIPEFEKIMPE
                     VRLMAWSPVLPLGPLPSNFPLYGSLRPFLAQLEAQGGTVFGDHDEPGHRYLLTGVKPR
                     RISDHLLLAVREDNFISAERTRTIRLMVGFTLGLFAVTIFLGYILSQRFLQPIGHLTT
                     GIEAIAERDFQHRLPPGDLDELGRLSETFNRMMEGLSDLEVGRIVQESLFPKDELVVG
                     AYRAFGQTHAATELGGDYYDVKRLPDDRILLIIGDVTGHGVGPAMVMAMAKIMVEYCL
                     SDFDLERFTVAFNDSLFKVMKRKRFMSCFLGIVDTKRHVMQMVNAGHNFPYLVRGSAH
                     LLEDLGNSYPIGSRASLKPKVVELPMEAGDTLLFYTDGLIETDTGATQIGYEGVAEAL
                     PRYLDADLPASCERIWAWNRELAQGKRQEDDITLLMLHRERNRG"
     gene            complement(4537..5277)
                     /locus_tag="PKO06_02715"
     CDS             complement(4537..5277)
                     /locus_tag="PKO06_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68583.1"
                     /translation="MKPKIRLGVTLIELLVALICFSLILLPAYHAFLMGTRTSLTGML
                     QVGTTMEARLALRQIHADLKGACLPFDARDLKITLDQLLSTSILGGETVYALQVFPRH
                     VEPTRTIVTEPNGLRPHVANRVTYSLETVKSGDPRKRLVRREKPHAGTGEAPRVTILS
                     ERVNHFEIRTVSFRGPVPRSAFFVTLQLIDSLDAAALSNLPTDPVGRPRGVVLADYCD
                     LVVPTFLSSMMAQEGVNRTWYSELRGPP"
     gene            complement(5274..5765)
                     /locus_tag="PKO06_02720"
     CDS             complement(5274..5765)
                     /locus_tag="PKO06_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68584.1"
                     /translation="MMMRLIKRRDARAFTLVEVVIALAFMGLALLGFLLVASSANRQS
                     MDAYYEFAALQAALEPIEVFRACGFELLENYASHPLDEYPTGWHALKDKPGSALQHPI
                     EWEMFQREITIDPPVRLPTAGNRRAIRVRVRVQPIQESRAASWLTRPGGVAVESLVVE
                     LPR"
     gene            complement(5762..>6443)
                     /locus_tag="PKO06_02725"
     CDS             complement(5762..>6443)
                     /locus_tag="PKO06_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68585.1"
                     /translation="GHPLFSGFDSSFLFGVGGGNVNQPPTDAAYPIGSLFGRRGNAIA
                     PDVTGTDGFRPFGGFGLWARRLDRASFLRSGMYDAVNNRLIIDGIVQILGNEVLSLGS
                     PDKPLIISGQGVIVASGFQIRGEMKRSDPANDLLILVTRRGNIVIDTTKPIEASLIAA
                     GDDLQRSIIPLKPMQVIGAVVVDRLNTHQWCAGEHHLRYDEALQRPQPLYRPAISRWV
                     VFERLAEE"
BASE COUNT         1412 a         1952 c         1779 g         1300 t
ORIGIN      
        1 tcgaagccaa agtgcgcaat cgactgaacc ctgagcgaat cgatgttgtc gggttcttcc
       61 tggcagaggc tgaggaagcc gcgcgtctgg cggcactcac ggctattcgc gctttgcgcg
      121 ccgagtatcc ggagaagacc gacatgcccg aagtgcagaa gtaccacttc cttggcgcaa
      181 actgtgtctc gtcgggcatt caaaacctga cccgctccat tcgcccggcc tatcatcact
      241 tcgcgaagca actggtgcgc aaggatgtcg acgagtttca ggagtggaac gccgctctcg
      301 cctgccagtg gatcaaatcc tggaacgaat tgactctgct ggccttcgac ggcctgcgcg
      361 gtggccgcat cgactggaag aaactctccg aggtgtctgc tttccacaag aacctgcgca
      421 gtcataatca gatgtccttg ccctggcgtg ccgaacccat catcaaatgg gttgccggaa
      481 agtaggacaa atcgagtgtc cttcagtccc gcagaagcac aatctgcttt tcgccgactg
      541 attcgtcaac cattcttcac ctatctgctc ctgattctcg ggttggcaac cgccgggatc
      601 tactcgacca tcgtgcgaga cgattttcag atcgtcaaag gcgcggaaat ggctctgctg
      661 gccattaccc gatttatcat cctcggacag ttgatcgaat tttttcgagg cctgatgatt
      721 ctcggcctgg gaactccact ggtgatggga ctgtcttcac ctctgtttta ttactctcgt
      781 caactccgac tggaaccatt tctcgaaacc ttcgggatcg gactcctccc cgggctgggt
      841 ctgttcttcc tgggctggaa aggtctgcaa ctgcatcgac gaaatcagca ggcagggccg
      901 ggtgctgagc cggagacggc cgctgatggg ttctgcgccc gctggttgcc aaccgtcgaa
      961 caagcctgcg aatggtcgat ctggatcatg atcgcgacgc tgatgagcgg tgaatggagc
     1021 ggcatcgggg ttctcgcggt tggcttcgcc ggcctgaccc tggcgttttc tctcgtgatc
     1081 ctcgtcggcg cgaaaacctc gattggccgg cgcggcatcg gcatctctct tgtgattgcc
     1141 ggtctggtca cgatcctcac ttcgatcgtc ctggtcacga cgcagggcaa tcgctgcacg
     1201 gaagccgttc tcggcatccc gcccggtatt ctgctcgtca tcatcggcct ggccctctgg
     1261 ctgggtgctt catcccgaca tgcgcgagtg agttagctcg gagacaattc tttacattcc
     1321 attacgaaaa cttgtcttga atgccacctt tcacgaggca tattctgtct gtgaccacca
     1381 cgacagaatg ccgcaaaggg ggcatacatg agctatcaga cggaacaacc acttccagct
     1441 cctcagcacc atgccacgac tttgcgcgcc atctgtctgc cgtgtccccc gatgtatcag
     1501 ggattcagct catggcagga actggcgcgt ctggtctggg aatgcgacct ctcttcctat
     1561 tcgctgctcg cagccttcga gacctggaaa cggtcggatg gttcgaaagt gggcctgctg
     1621 aaacttccca cgcgccccga gtatctccgc cgcaggcacg aagttacccg cgattccgct
     1681 cgcgatggag catcaggaga gtgatgtcgt cttcctggcg ctttccctgc gccagttccc
     1741 ggttccaggc ccagatacgt tcgcatgatg cgggtagatc ggcatcgaga tatcgaggca
     1801 aggcttcagc cacgccttca tagccgatct gcgtggcacc ggtatcggtc tcgatcaatc
     1861 cgtccgtgta gaacaacagc gtgtcgccgg cttccatagg cagttccacg accttgggtt
     1921 tcagactggc gcgactgcca atcggataac tgttgcccag atcttccaag aggtgtgccg
     1981 aaccccgcac cagatacgga aagttgtgtc cggcattcac catctgcatc acgtgacgct
     2041 tcgtatccac gataccgaga aaacagctca tgaatcgttt gcgtttcatg actttgaaca
     2101 acgagtcgtt gaacgccacc gtgaatcgtt cgagatcgaa gtcgctcaaa cagtattcca
     2161 ccatgatttt cgccatcgcc atgaccatcg ccgggcccac gccgtgtccg gtgacatcgc
     2221 cgatgatcag cagaattcga tcgtcgggca aacgcttgac atcataatag tcgccgccga
     2281 gttccgtagc ggcgtgtgtc tgtccgaatg cgcgataggc accaaccacc agttcgtcct
     2341 tcggaaaaag gctttcctga acgattcgac caacttcaag atcggacagt ccctccatca
     2401 tgcgattgaa cgtttcagat aatcgtccga gttcatcgag gtcaccaggc ggcaatcgat
     2461 gttggaaatc acgttcagcg attgcctcga tacccgttgt caaatggcca atgggttgca
     2521 gaaatcgctg tgacagaata tagccgagaa agatcgtcac ggcgaacaag cccagcgtga
     2581 atccaaccat caggcgaatg gtccgcgttc gttccgcgga gatgaagtta tcttcgcgca
     2641 cggcgagcag cagatgatca gagatgcgac gaggtttcac accggtcagc aggtatctgt
     2701 ggccgggctc gtcatgatca ccgaagacgg tacctccctg cgcttccagt tgtgccagaa
     2761 atggtcgcag tgagccatac agcggaaaat tcgatggaag aggtcccagc ggaagcacgg
     2821 gactccaggc catcagtcgc acttccggca tgatcttctc gaactccggt atcgcccgct
     2881 gcagatacaa ccgctccagc agatcccttt tccaggaaac ggaaacgaca cgctcgatcc
     2941 gaccgccggc actgcgcatc ggttgcagaa aaatccagtg ctggtctcga aaaaccgtga
     3001 tctcgaaaac ctttccgagg ttcgacacca tttcaccaac cgggagttct cctccggtga
     3061 actgttccgc gatcgagacg gccatttcgc cactcacgct ctcttcgacc ggctgcaacc
     3121 gggaacagag ggtgcggatc agactgccaa gcagacgcgt agagccttct tctcgaatgt
     3181 ttttctccat ccacggcgga ttcgcgtttt cctgataccg cagcagcact ttgccgttgg
     3241 ggttgaacac atagatttcg cgcggagaga acgaacggcg cacctcttcg agtacctctc
     3301 gccagttctt ccgggcttcc aggtcggcga agcgatccgg tcgcagcatc tgtccgagtt
     3361 tcagttcgat atcggcgcgc agattcgcat agcggttatc caaagcctga agatgtcgct
     3421 ccatggcttc ggtcgcttct cgacttcgtc gacgttccag gtcctgcagg gcatcccatt
     3481 gggcgaacgc aatcaacacc agcggcactc cgccagcaaa cgcaaacagg agcagaagac
     3541 gctgacggat ggaaagcaca aacgtgcttt ggcccagcat gactctggcg gaaaccaggc
     3601 acaacacact gaagacggca gtgacccaga aaatcaggcg caggcggcgc gaatcaaagt
     3661 ctcccaggag tcgtcgcgga atcgaggccc agaggaacac accggcatcg atctgctgca
     3721 acgtcaccag cgatgccggc aacagggcgt gttgccgggt cgtcaccccg tactgtcgca
     3781 gagcgacggc cacatccggc ggagcgtagg cgtgtcgacg cagatccacc aggccgaaat
     3841 ccacctgacg cttcggacgc agctgccgca acaaccggat ctggtcgttc aagggcgcct
     3901 ggtcccagtc gggaaccatc cgaacctgaa tgcagaatcc cccccacttg cctgtggcaa
     3961 acgagagcca gcgccactca ccaccgggat tggcatcgac cggcttcaac ttcgccccct
     4021 gcgccagttg ctccggttcg acaccggggc ccaggaacgc actcagtatc ggtcgatagc
     4081 gcttcgcggc ttccaggcgg gtttcgtcct ctccgcacag cacttcgctc aaacgccgca
     4141 cgatggaatc cggcacggag ggatcaagct ctggacacag tctgcgaccc tcgcgatcaa
     4201 acatgatgat gcgcagcatg ccgggaaaac gagcatgaaa tgtggcgatg gccttgcgca
     4261 tccgttccgt tctctccctg aatggggatc ggcgaatgaa ctgcttcaag gcatcaaact
     4321 gtgcgcccag atacacgatg gtattcgagc gcttgcgaat cagcgccaga tgccgatcaa
     4381 ggcgagtgaa cgcctggtcg tgccgttcgc gttcctggcg ctcccacatg cgaccaaacc
     4441 ccgccgtcac gatcagcaaa ggaatggcaa agaaaatgag cacctgtccg agccagcgca
     4501 gcaggggcag gtgagcacga agaatagagc gaagactcat ggcggaccgc gcagttcaga
     4561 gtaccaggtg cgattcacgc cttcctgcgc catcattgat gagagaaagg tcggaaccac
     4621 gagatcacag taatcggcaa gaacgacacc gcgcggccgg ccgacagggt cggtcggcag
     4681 gttactcaaa gcggcggcat caagcgagtc gatcaactgc agggtcacga agaaggcgga
     4741 gcgcggaacg gggccgcgga aactcacagt gcgaatttcg aagtgattga ctcgctcaga
     4801 gagaatcgtg acccgaggag cttcccccgt gccggcatgc ggcttttctc ggcgcaccag
     4861 acgcttgcgc gggtcaccgc ttttcaccgt ctcgagagaa tacgtcacgc ggttcgccac
     4921 atgcggccgc aagccgttgg gctcggtgac aatcgtccgc gtcggttcca catgtcgcgg
     4981 aaacacctgc agagcgtaga cggtctctcc tcccagaatg ctcgtggaca gcagctggtc
     5041 gagcgtgatc ttcaagtctc tcgcatcgaa tggcagacag gcccccttca gatcagcgtg
     5101 gatctgacgc aaggcaaggc gtgcttccat ggtggttccc acctgcagca tgccggtcag
     5161 cgaggtgcga gtccccatga gaaaggcatg atacgccggc aggagaatca gcgagaagca
     5221 gatcaacgcg accagcagtt cgatcagcgt cacgccaagc ctgattttcg gtttcatcgc
     5281 ggaagctcca ccacgaggct ttcaacggcg acgccgcccg ggcgagtcag ccagctcgcg
     5341 gcacgacttt cctgaatcgg ttgcacccgc acgcgaactc tgatggcgcg ccgatttcct
     5401 gccgtcggca atcgtaccgg cggatcaatg gtgatttccc gctggaacat ctcccactcg
     5461 atcgggtgtt gcaacgcaga cccaggtttg tctttcaacg cgtgccatcc cgtggggtat
     5521 tcgtcgagcg gatgagacgc atagttttcg agcagttcga aaccgcacgc ccgaaacacc
     5581 tcgatcggct caagggctgc ctgcagtgcg gcgaattcgt aataggcatc catcgactgc
     5641 cgattggcac tcgaagccac cagcagaaac ccaagcaacg ccagccccat gaatgccaga
     5701 gcgatgacga cttcgaccag cgtgaacgct cgcgcgtctc ttcgtttgat caggcgcatc
     5761 atcattcctc tgccagtctc tcgaagacga cccagcggct gatcgcaggt cgatacagcg
     5821 gttgcgggcg ctgcagagct tcatcgtacc gaaggtgatg ttcccccgca caccactgat
     5881 gggtattcag acggtcaacc accacggccc cgattacctg catgggtttc aagggaatga
     5941 tcgatcgctg cagatcgtcc ccggcggcaa tcaatgaagc ttcgatcggt ttggtagtgt
     6001 cgatgacgat gttgcctcgc ctggttacca gaatcagaag gtcattcgcg ggatcgctcc
     6061 gtttcatctc accccgaatc tgaaaccccg atgccacgat gactccctgg ccggaaatta
     6121 tcagcggctt atccgggcta ccaaggctca acacttcgtt gccgagaatt tgtacaattc
     6181 cgtcaatgat cagacggtta ttcacagcat catacatgcc actgcgaaga aatgacgcac
     6241 ggtcaagccg tcgagcccag aggccgaatc cgccgaaggg acggaagcca tccgtaccag
     6301 ttacgtctgg agcaatggcg ttccctcggc ggccgaacag agaaccgata ggatacgccg
     6361 cgtctgtcgg cggttgattc acgtttcccc cgccgacgcc aaacagaaat gaggaatcga
     6421 agcccgaaaa caaaggatga cca
//
LOCUS       DAORHD010000075         6443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_11984_length_6443_cov_3.303538, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000075 DAORHD010000000
VERSION     DAORHD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11984_length_6443_cov_3.303538"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PKO06_02730"
     CDS             <1..770
                     /locus_tag="PKO06_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68586.1"
                     /translation="YFQVLQQIRRMHAFDLTQLPSGMQLAEGYSEKLYRWEQNYFLEN
                     FVGAVCQLDLAPAFIRDLEQELDALTCRLQKLEPSLIHRDLQPGQTLTTLGVYRPSSR
                     ELRVNGIVWAGDYLELGDGADLPVTIRGQGVLMARSIRVRSGLVKATPDDLCVLVARD
                     GPIRIDTESEIQAALVAVNDRRDGRIFTRRRFQLTGSLAVDRLCSSYDPITGQIELVP
                     GPSDWNLRYDPIFRPRKPQLQVALAHWVLFERFEREP"
     gene            767..1228
                     /locus_tag="PKO06_02735"
     CDS             767..1228
                     /locus_tag="PKO06_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68587.1"
                     /translation="MKSTCRRGFSLIEALTGLCILVVLLLGIVQMNAQSRHVAGDAVY
                     ELQAAQLAEEGIEILHAFGYDWVSRYVEHPVSGLSLTWAPIVESPVAGDEYPAEYRQF
                     QRKVQVTPIETERLRACRIEVQVAPLPGTTAEQGLSRVPVTAETLVLEMPR"
     gene            1225..1944
                     /locus_tag="PKO06_02740"
     CDS             1225..1944
                     /locus_tag="PKO06_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68588.1"
                     /translation="MIREQRRGVSLVELLFGVVISGLVVWLLFLVLHTETRLTRRTQQ
                     RTEAAREARQILDWLRDDLVHCCQPLSGGRFRPSLVDALERRSDGGLDTFSFLCFSRE
                     LPLADAVPLRRFGKSHRQASRITWRLVQPSQPSADGCLALERIVQMPTTGTSDLKRAG
                     YLKRLSERVLAFSIRPQTITAQGERVQIFRVSVRLRHADTGGTSGGVCEMFEVVCPDF
                     FQSTWSQHGIRHPWHAIMSGP"
     gene            1969..4842
                     /locus_tag="PKO06_02745"
     CDS             1969..4842
                     /locus_tag="PKO06_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68589.1"
                     /translation="MKDTLFLRLFRWVIFCVCFIAFPLALLNYGLHMGLARHWEAYTN
                     SEARRVTMRMEQILRASKDREFYYELIDRCASHLAASADRPLLQKRLAQHFMKRFDGL
                     IDLYITNPKGEMVFPDDLPPGVSRFAVRKLHEGFAELPSIRGLLRNLKILWYFLSDNP
                     SNEDFPQMDVTRRMAEIYPMGRRSLLYGRIFPQGGVFGQVHKGAREAWFPVTLLAEKS
                     NQKGQAMTLGLYSRERVSSETPLKANEVSVVTQGLAAIDHSARDWFVRDHLLVRVMTL
                     DSNNKFWGLVRLDQQREGRLWKSRALVYSILLFFSIAIVSFCLLVLDWRIWFSIRWKL
                     IGLFGIATGLPLVVLFVKGYDLLQSTEQNLKAEALTNLQESVKSLDLKFPQITRDYVA
                     LFGRLQKSITKPNDRQQLLAFFDELVRRREELGYNMPLILQRDGTIIVRDDRDRKNVD
                     RFLMALGMELMTRFNKAMGFKEEAEVQTARSVEAAMLSDKNAVGLVHGLFKNMGQLAR
                     MSFGRSDRFLYLSVFQDDRGMAHSIYFMSIPSKRIFHSYLQKYLLVRQREVPNSRLFA
                     FHEDDANLHVPKAIHRESWLVNFVDRVRSRRGVVADELEIEGQPCMAVGIPGSQVDQY
                     CLVQVLPLTELVAAKQQLSVQLLLFGVLCLALSMSVGFMLSGQFLRPIAALADGVQAI
                     NEGRFQHRVDRLSDDELGQLSNSFNVTMERLEELSVAHTVQESLFPATKLELGGMEVF
                     GQSIAATELGGDYYDYFSVDDKRLVILIGDVAGHGTASALLMAMAKATITVEVRNNPH
                     PAHMLEVVNQMIFTAMRKKRMMTFFYALVDTSTGEIEYVNAGHNSPFLLRVRDGQFLE
                     LASESYPLGTRKTMVYKQRKIDLAPGDSLWCYTDGFPECACEGRDQLGYDLFRQVLRE
                     SRGADAFERCQAVYRRINAMRGMVPQNDDMTMILLQHRGASAR"
     gene            4861..5424
                     /locus_tag="PKO06_02750"
     CDS             4861..5424
                     /locus_tag="PKO06_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNV68590.1"
                     /translation="MAFDSPFVVFSIAVALGCDALAVGLAVGTRNPDFRARFRLWFHF
                     GLFQFFMPLVGWYIGRKTLAYIEAYDHWIAAGLLWVIAAKMLKESLSNDDEEANSRSA
                     SDPTRGWSLVALSFATSFDALGVGFSLSMARTPLLVPCVIIGLMAGTMTYTGLRLGTR
                     LSAAFGKRVEMFGAGILFYIGYRLLEI"
     gene            5452..6396
                     /gene="trxB"
                     /locus_tag="PKO06_02755"
     CDS             5452..6396
                     /gene="trxB"
                     /locus_tag="PKO06_02755"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNV68591.1"
                     /translation="MTRKLVIIGSGPAGLTAAIYAARAGLSPLLFEGQQLGGQPGGQL
                     MTTTEVENFPGFSKGIMGPDLMMEMRAQAVRFGAEILTEDVETVDFSRRPLTLKNAQR
                     EIQAHAVIVATGASARILDLPAVKAFWGKGVSACATCDGALPFFRNQPLAVIGGGDSA
                     IEESLFLTRFGSEVHIIHRRDQFRASRIMSERAQKHPKIKIWWDSVVEDLAGDQALKT
                     VKIRHVKTGQVSDLPCRGLFMGIGHQPNTAFLKGALNADEQGYIQVRHPTTWTSVEGV
                     FACGDVIDPHYRQAVAAAGTGCIAALDAERWLAANNLG"
BASE COUNT         1265 a         1722 c         1980 g         1476 t
ORIGIN      
        1 attattttca ggtattgcaa caaatccgca ggatgcatgc ttttgatctc acccaactgc
       61 catcaggcat gcagttggcg gagggctaca gcgaaaagct gtaccgctgg gaacagaatt
      121 attttctgga aaactttgtc ggcgcggttt gccaattgga ccttgctccg gcttttatca
      181 gagatttgga gcaggaatta gatgctttga cctgccgcct gcaaaaacta gaaccgtccc
      241 tgattcatcg tgatctgcag cccggacaga cgctcaccac gcttggcgta tatcgtccgt
      301 cgagccgtga gcttcgcgtg aacggcatcg tctgggccgg ggattatctc gaacttggtg
      361 acggtgccga ccttcccgtg accatcagag gccagggggt actgatggct cgcagtattc
      421 gcgttcgcag tgggctggtc aaagcgaccc ccgatgatct ttgtgtgctt gtggctcgtg
      481 atggtccgat tcgcatcgac accgagtctg agattcaggc cgccctggtg gcggtcaacg
      541 atcggcgtga cgggcgcatc tttacccggc gtcgatttca gctgaccggc agtctcgcgg
      601 tcgatcggtt gtgttcctcc tatgacccga ttaccgggca gatcgagctg gtaccagggc
      661 ccagcgattg gaatctgcgc tatgacccga tattccgccc gcggaagccg cagctgcagg
      721 tggctctcgc acactgggtg ctctttgaac gattcgagag ggaaccatga agtcgacatg
      781 ccgccgtggc ttcagcctga ttgaggcgct gaccggactg tgcattctgg tcgtgcttct
      841 gctgggcatc gtgcagatga atgcccagtc gcggcacgtg gcgggcgatg cggtgtatga
      901 gttgcaagcc gctcagcttg ccgaagaggg aatcgagatt ctgcatgcat tcggatatga
      961 ctgggtgtca cgctatgtgg agcatccggt ctcgggcttg tctctgacgt gggcaccgat
     1021 tgttgaaagc ccggtcgctg gtgatgaata cccggcggag taccggcagt ttcagcgaaa
     1081 agtgcaggtg acgccgatcg agacggagag actgcgagcc tgtcgcatcg aggtgcaggt
     1141 cgccccattg ccgggaacca ctgccgaaca ggggttgtcg cgcgtgccgg tcaccgcgga
     1201 gactctggtg ctggagatgc cgcgatgatt cgagaacaac gacgcggggt aagtctggtg
     1261 gaactgctgt tcggcgtggt gatctcggga ttggtggtct ggctgctgtt tctggtcttg
     1321 catacggaga cacgcctgac caggagaacc cagcaacgaa cggaggctgc ccgggaagca
     1381 cgacaaattc tggactggct gagggatgat ctggttcatt gttgtcagcc gctttccggg
     1441 gggcgctttc gcccgagtct tgtggatgca ctcgagcgaa gaagcgacgg agggctcgac
     1501 accttttcgt ttctctgctt ttcccgcgag ctgccgctcg cggatgccgt tcccctgcgt
     1561 cgcttcggaa aaagtcatcg acaggcgtct cgcatcacct ggcgacttgt tcagccttca
     1621 cagccttcag cagatggatg tctggctctt gaacgaattg tacaaatgcc gacgacggga
     1681 acgagcgacc tcaagcgtgc tgggtatctg aagcgtctca gcgagcgggt gctggcgttc
     1741 agcattcgac ctcagaccat cacggcgcag ggggaacggg tgcagatctt tcgggtttcg
     1801 gtgcgcctgc ggcatgcgga tacaggtgga accagtggcg gggtgtgcga gatgttcgaa
     1861 gtggtctgcc ccgacttctt tcagagtacc tggagtcagc acggaatccg ccatccgtgg
     1921 catgcgatca tgagcgggcc gtgacgagat gaaacaggac atcagacgat gaaagacacc
     1981 cttttcctgc gcctctttcg ctgggtgatc ttttgcgtct gtttcatcgc ctttccgctc
     2041 gctctcctga attatggtct gcacatgggg ctggcacgtc actgggaagc ctacacgaat
     2101 tctgaggctc gacgggtcac catgcggatg gagcagattc ttcgcgcttc gaaggatcga
     2161 gagttctact atgaactgat cgatcggtgc gccagccacc tggcggcgtc ggccgaccgt
     2221 ccgcttctgc agaaacgact cgcgcagcat ttcatgaagc ggtttgacgg tctcatcgac
     2281 ttgtacatta ccaatccaaa gggagagatg gtttttcccg acgatcttcc gccgggtgtc
     2341 agtcgcttcg ctgtgcgcaa gctccatgag ggttttgcgg agctgccgag cattcggggc
     2401 ctgctgcgca acctgaagat tctctggtac ttcctcagtg acaacccgag taacgaggat
     2461 tttccgcaga tggatgtcac tcgacgaatg gcggaaatct atcccatggg tcgcaggtcg
     2521 ttgctgtatg gccggatttt tccgcagggc ggggttttcg ggcaggtgca taaaggtgcg
     2581 cgtgaggcgt ggttcccggt cacgttgctg gcggagaagt cgaatcaaaa gggtcaggcc
     2641 atgactctcg gtttgtacag ccgagaaaga gtgagctcgg agactccgct caaagccaat
     2701 gaagtatccg tggtcaccca gggactggcc gcgatcgatc attcggcgcg ggactggttt
     2761 gttcgagatc acttgctggt gcgagtcatg actcttgact ccaacaacaa attctggggc
     2821 ctcgtgcgcc ttgaccagca gcgggaaggg cgactctgga aaagccgtgc tcttgtgtat
     2881 tcgatcctct tgttcttttc tattgccatt gtcagctttt gcttgttggt actcgactgg
     2941 cgcatctggt tttccattcg ctggaaactg atcggtctgt tcggcatcgc gaccgggctg
     3001 ccgctggtcg tactcttcgt caaaggctac gacttgttgc agagtactga gcagaacctg
     3061 aaagccgaag cgctgacaaa cctgcaggag tccgtgaaaa gtctcgacct gaagtttccg
     3121 cagatcaccc gtgattatgt tgccctgttc gggcggctgc agaaaagcat taccaagccc
     3181 aatgatcgac aacaactgct ggcctttttc gacgagctgg tacgtcgcag ggaagaactt
     3241 ggttacaaca tgcccctcat ccttcaacgt gacggcacga tcatcgtccg ggatgaccgc
     3301 gatcgaaaga acgttgatcg gtttctcatg gccctgggca tggaactgat gacacgcttc
     3361 aacaaggcga tggggttcaa ggaggaggcg gaagtccaga cagcgaggtc ggtcgaagcg
     3421 gcaatgctga gcgataagaa cgcggtcgga ctggttcacg ggctgttcaa gaacatgggc
     3481 caactggcac gcatgtcttt cggccgttct gaccggtttc tctacctgag tgtttttcag
     3541 gatgaccgcg ggatggcaca cagcatctac ttcatgtcga taccctcgaa acgcatattt
     3601 cacagttatc tgcagaaata cctgttggta cgccagcgtg aagttccgaa cagccgactg
     3661 ttcgcttttc atgaggatga tgcgaatctg cacgttccca aggcaattca tcgagagtcc
     3721 tggttggtaa acttcgtgga ccgcgtcaga agtcggcgag gcgttgttgc agatgagctc
     3781 gaaatcgagg ggcaaccgtg catggctgtg ggaattcccg gcagccaggt cgaccagtac
     3841 tgcctcgttc aggtgttacc actcacggaa ctggttgccg ccaaacagca gttgtcagta
     3901 caactgctgc tgtttggcgt gctttgtctg gccttgtcga tgtcggtcgg gttcatgctt
     3961 tcggggcagt ttctccgacc gattgccgcg cttgccgatg gggtgcaggc gatcaatgaa
     4021 gggcgtttcc agcatcgagt cgatcgtttg tcggatgacg aactcggaca gttgtcgaac
     4081 tccttcaatg tcaccatgga acgcctcgag gagctgtcgg tggcacatac ggttcaggag
     4141 agcctgtttc cggcgaccaa actcgaactg ggagggatgg aggtattcgg gcagtccatc
     4201 gcggcaacgg aactgggcgg agactattac gactatttct cggtcgacga caagcgtctg
     4261 gtcattctga tcggcgatgt ggccggacac ggtaccgctt cggcgttact gatggcgatg
     4321 gcgaaagcga ccatcacggt cgaggtgcgg aataacccgc acccggcaca catgctcgaa
     4381 gtcgtgaacc agatgatctt cacggccatg cgcaagaagc gcatgatgac cttcttttat
     4441 gccctggttg atacgtcgac tggcgagatc gagtatgtga acgcgggaca caacagtccc
     4501 ttcctgcttc gggtgcgaga cgggcagttc ctcgaactgg cgtcagagag ctacccgctg
     4561 gggacgcgca agaccatggt gtacaaacag cgcaagatcg atctggcacc cggtgacagt
     4621 ctgtggtgct acacggatgg attccccgaa tgcgcgtgtg aaggccgtga tcaactcggc
     4681 tatgacctgt ttcggcaggt gctaagggaa tcgcgtgggg ccgacgcgtt cgaacgttgt
     4741 caggccgtct accggcggat caacgccatg cgtgggatgg tgccgcagaa cgacgacatg
     4801 accatgattc tgttgcagca tcggggagcc tcggcgaggt gatatgttat actcgccgcc
     4861 atggctttcg attcgccctt tgtcgtcttc agcattgcgg tcgctctggg gtgtgatgct
     4921 ctcgcggtcg gcctcgccgt cggtactcgc aaccccgatt ttcgcgcgcg ctttcgcctg
     4981 tggttccact tcgggctgtt tcagttcttc atgcccctgg ttggttggta cattggccga
     5041 aagacgctcg cttacattga agcctatgac cattggattg cggcaggtct gctctgggtc
     5101 attgccgcga aaatgctgaa agaaagcctg tcgaacgacg acgaagaggc aaattcgcgc
     5161 agtgcaagcg acccgacacg tgggtggtcg ctggtggctc tctcgttcgc taccagtttt
     5221 gacgcgttgg gcgtgggttt cagcctgagc atggcgcgaa caccgcttct cgtgccctgt
     5281 gtcatcattg ggctgatggc cggaacgatg acctatacgg ggctgcgttt gggaacccgt
     5341 ctcagtgccg cattcggcaa acgagtcgag atgttcggcg ctggaattct gttctacatt
     5401 gggtatcgcc tgctggaaat ctgaatcgag aacctcaagg gaggcatgaa catgactcgc
     5461 aaactcgtta tcatcggttc cgggcctgcc ggcttgacgg ccgctatcta tgcggctcgc
     5521 gccggcttgt cgcctctact gttcgaagga cagcaactcg ggggacagcc aggtggacag
     5581 ctgatgacaa ccaccgaagt cgaaaacttt ccgggttttt cgaagggtat catgggaccg
     5641 gatctgatga tggaaatgcg tgctcaggcg gtacgtttcg gcgcggagat cctgaccgag
     5701 gatgttgaaa ccgtggactt cagccggcgt ccgctgacgt tgaagaacgc tcagcgtgag
     5761 attcaggcgc atgcggtgat cgtcgcgacg ggcgcgagcg ctcgtattct cgatctgccc
     5821 gcggtaaagg ccttctgggg aaaaggagtt tctgcctgtg ccacgtgtga cggtgctctg
     5881 ccatttttcc gcaaccagcc gctcgccgtc attggcggcg gtgactcggc gatcgaggaa
     5941 tcgctttttc tgacgcgctt cggatctgaa gtccatatca ttcatcgtcg cgatcagttt
     6001 cgcgcaagca gaatcatgtc agaacgcgca cagaagcatc cgaagatcaa aatctggtgg
     6061 gacagcgtgg tggaagatct ggctggtgat caggccctga agaccgtgaa gattcgccac
     6121 gtgaaaaccg gtcaggtcag cgatcttccg tgccgtgggt tgttcatggg tattggtcat
     6181 caacccaaca cggcatttct caagggcgcg ctcaacgcgg acgagcaagg ctacattcag
     6241 gtgcggcacc ccacgacctg gacgtcggtc gagggagttt tcgcgtgtgg cgatgtcatt
     6301 gatccgcact atcgccaggc ggtcgcggcg gctggaaccg gctgtatcgc cgccctcgat
     6361 gccgaacgct ggcttgccgc caacaatctg ggctgatcgc cagccgttcg tcagatccgc
     6421 agcccgacca cctcgaacat gcg
//
LOCUS       DAORHD010000076         6433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12007_length_6433_cov_4.190185, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000076 DAORHD010000000
VERSION     DAORHD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12007_length_6433_cov_4.190185"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKO06_02760"
     CDS             <1..668
                     /locus_tag="PKO06_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNV68592.1"
                     /translation="SSQFRILRVFKNRFGATGEVGVFEMTGKGLLPVLNPSDLFVNRS
                     RGDSPGVAIVPLLEGSRCILAELQALVTQSFLAMPRRVVAGLDSNRLHLVIAVLEKHA
                     GLRFYNKDVFANVAAGLRLSEPGGDLGLAMALVASLRNQPIPREILCTGELTLSGEIR
                     PVSGLDRRLNEGRRFGFKKFLVPARADLPKAADLVPVTHIREAIQTVFPGRPPEPREI
                     EPE"
     gene            771..1859
                     /locus_tag="PKO06_02765"
     CDS             771..1859
                     /locus_tag="PKO06_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HNV68593.1"
                     /translation="MLFNLIRFILVITGTVVGVALGYGLIDQYGNFLEIEHPGLKFAA
                     LLGCLGYLLSSMAGRELDLYLQAKIETTNSYDLAWAALGLVFGLISANLLLIPVYFIL
                     YKDLVHFRFDNQYFNSLVPLITLVIPVFFNLLFAYLGASIVSRYRRGIPRHLPAISSV
                     PPKALDTSVIVDGRIADLIRMSFMEGTFLVPRFVLNELQFLADSGDQLKRKRGRQALD
                     LLKALRKEYPDNFLLTEKDYPQVEEVDAKLIEFTRDEAAVMVTLDFNLKRVAELQNVK
                     VLYLQDLVSIFKPPVQTGEELEIQILKPGKETEQGVAFLPDGTMVVVEDGGHDIGKKV
                     KITVTNILQTTGGRMVFGRIHLKKKAGS"
     gene            1887..2666
                     /locus_tag="PKO06_02770"
     CDS             1887..2666
                     /locus_tag="PKO06_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HNV68594.1"
                     /translation="MAALRVKDIMDPHPVVVTADQTIFGLKEIMILRQPECILVVDKA
                     NRLVGMLTSAHVAGAMGIEDRVDSIMVHNCTIIRDTELVRDAAARLSASDFEALPVLD
                     ASDHIVGVVSYKDIVKDLVDEPAQKLSPESAVVYLAMTRDPEQEEYWLEKVTANGYRA
                     AITQVGATAEKLPIKLREATIVAAIARSVIREDPREKFAVSNAVRDIYSQCNVINPGL
                     GGGFKVAIVRGEGRIAVAAFGRCGHALANGNEQIFMGGNII"
     gene            2667..4097
                     /gene="gltX"
                     /locus_tag="PKO06_02775"
     CDS             2667..4097
                     /gene="gltX"
                     /locus_tag="PKO06_02775"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007391344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNV68595.1"
                     /translation="MSSPIRVRFAPSPTGYLHVGGARTALFNWLLARQTGGVFILRIE
                     DTDLERSTAESEEGLLRDLRWLGLDWNEGPGVGGPHGPYRQSERTTIYQDAARRLMAK
                     GAAYPCFCSEESLEAKRKQAEASGAAMHYDGTCTRLPAAEVQRRISAGEPYAIRMKVP
                     EKDYVLDDLCRGSVEWKSENLGDFIILRSNGMPVYNFCVVVDDAEMEVTHVIRGDDHL
                     TNTHRQLIIYEALERPRPKFAHVSMILGPDKAKLSKRHGTTSVGQYAEDGFLPEAMVN
                     FFALLGWNEGTNQELYSRDELIQKFSIERIGNSAAVFDHAKLLWMNGEHIRKLSLDTL
                     TQLLEPRFKKAFPGDVRFDDPAFRQRATQVIQPALKTLNDVGQILTPILHGGTPENAE
                     AEEALKGPDVPRLLEALQPVFGEIEWKKEIINQTIKDLAKKLNIKGKALFMPIRVRLT
                     GNCHGPDLVGIIELLGRETVLQRLKP"
     gene            complement(4171..5226)
                     /gene="phnD"
                     /locus_tag="PKO06_02780"
     CDS             complement(4171..5226)
                     /gene="phnD"
                     /locus_tag="PKO06_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV68596.1"
                     /translation="MARGTWLLIVFVVLAAVTIGFWWNRTSDPEAIRITWEIASAPPL
                     IMGVIPYLPEQLLKREFHALTGYLSKNLHREVQLNFAVDYESLGRLLDLGKVQLAWFS
                     ANSFESLNRENRWESICRPLREGAVRHRGAIVVREGSGIRTLAELRGRRFAYVDRHSG
                     TGFVMPNQLFRAEGIDPLRFFSEVTFTGNHSTSISGVLSGAYDAAAVYDVYSGHALWA
                     LDLKTQKTIPVPVSSESPVVMEASHTRLVPGSGFAHEGLFCIAFTEWLLNDPVAVRRD
                     MDATFKEQIRDLLVNMKKYPGGSEVLQELSQLRKWNGFIDERSLAETIASQDWALGRP
                     QSLATETASSVTPLRER"
     gene            complement(5202..>6433)
                     /locus_tag="PKO06_02785"
     CDS             complement(5202..>6433)
                     /locus_tag="PKO06_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68597.1"
                     /translation="YSIEDLLLHEQDPRLVADIIETLHAGDTERMTQILQRFLDHSHH
                     RVRGSAVQTLLRRCDQRPVLTKCLEHLASLLSSTLSVERATAAVVMGATGLPVFVPAL
                     GVLADDEQAEVSRHACRSLARIGSSQAVRLLAEKRIMGRHTAAAQSAWELLNREGMEE
                     RAKVLARLSAAERSRIGFWLKAVQSSADDELLMRVLRVSESQKRETLLRALGSEDADR
                     DLLANCLCLEGTEIRLDPEPLLERLLLQDLDRFPTWGDLIIPLCGPGHAGYERVLLVM
                     LQRLAREMCIYERGCSVGLWKDEQRKNNLDKWLDNRLRVVIHFTALAGKQPAETLEAL
                     VKATTSDRFVHSVALEFLEEQLGRTIAAHLFPLLEPGDPMTRQARLASTAGIDLQELD
                     QAHLEEEVRTWLAEPGF"
BASE COUNT         1369 a         1957 c         1750 g         1357 t
ORIGIN      
        1 tctcatcgca gttccgtatt ctgcgtgtct tcaagaaccg tttcggcgcg actggggaag
       61 tcggcgtgtt cgagatgacc gggaaagggc tgctgcccgt gctcaatcct tccgatctgt
      121 tcgtgaaccg gtctcgcggt gattcccccg gtgtcgcgat tgttccgctt ctggaaggct
      181 cgcgctgcat cctcgcggaa ctgcaggccc tggtcaccca gtcgtttctg gccatgccac
      241 gccgagtcgt cgccgggctc gacagcaatc gcctgcacct ggtgatcgcg gtgctcgaaa
      301 aacacgccgg tctgcgcttc tacaacaagg atgtctttgc gaacgtcgcg gctgggcttc
      361 gtttgagcga acccggcggt gatctgggtc tggccatggc cctcgtggcc agcctgcgta
      421 atcagccgat tccgcgcgag attctctgta ccggcgaact tactctctcc ggagagattc
      481 gaccggtttc cggactcgat cgccgactta acgaaggtcg ccggttcggt ttcaagaagt
      541 ttctggtgcc cgcccgcgcc gacctgccga aggccgcgga tctcgtgccc gtcactcaca
      601 tccgcgaagc tatccaaacc gtctttcccg gccgcccacc cgagccgcga gagatcgagc
      661 ccgagtaggt ccaatccact cctgaagccg atctccggtt cctcgcccac cgcgagaacc
      721 ttgcgaaagg ttctggtttg tgctaagctg tcgcgccctc aggggtatct atgcttttta
      781 acctcattcg tttcattctg gtcatcaccg gcaccgtcgt cggcgtcgcg ctcggatatg
      841 gcctcatcga ccagtacggc aacttcctcg aaatcgaaca tcccggtttg aagttcgccg
      901 ccttgctggg gtgccttggg taccttctca gctcaatggc tggtcgcgaa ctcgatctct
      961 acctgcaggc caagatcgaa accaccaaca gttacgatct cgcctgggct gccctggggt
     1021 tggttttcgg gttgatctcc gccaacctgc tgctcattcc ggtgtatttc atcttgtaca
     1081 aagatctggt tcactttcgc ttcgacaacc agtatttcaa ctccctggtt ccactgatca
     1141 ctctggtgat tcccgtgttc ttcaatctgc tgttcgccta tctcggtgct tcgatcgtca
     1201 gccgctatcg acgcggcatt ccgcggcacc tgccggccat ctcttcggtg ccgccgaaag
     1261 ccctcgatac cagcgtcatc gttgatggcc gcatcgccga tctgattcgc atgagcttca
     1321 tggaaggcac attcctggta ccgcgattcg ttctcaacga gttgcagttt ctggccgatt
     1381 ccggcgatca gctcaaacgc aagcgtggtc ggcaggctct cgatctattg aaagctctgc
     1441 gcaaagagta tccggacaac tttctgctca cggaaaaaga ctacccgcag gtagaagagg
     1501 ttgatgccaa gctgatcgag ttcacccgtg acgaggctgc ggtgatggtc actctcgact
     1561 tcaacctgaa gcgcgtcgcc gaactgcaga acgtcaaggt gctctacctt caggatctgg
     1621 tcagcatctt caaaccaccg gtacaaaccg gtgaagaact cgagattcag attctcaagc
     1681 ccggcaagga aacggagcag ggggtggcat ttctgcccga cggcaccatg gtcgtggttg
     1741 aagacggtgg ccacgatatc ggcaagaagg tgaaaatcac cgtcaccaac atcctgcaga
     1801 cgaccggtgg ccgcatggta ttcggccgca ttcatctaaa gaaaaaagct gggtcataaa
     1861 cccgacccac gacagggagg accgacatgg ccgcgttacg agtcaaagac atcatggatc
     1921 cgcatccggt ggtggttacc gccgatcaaa cgattttcgg gctgaaggaa atcatgattc
     1981 tgcgtcagcc ggaatgcatt ctcgtggtcg acaaggccaa ccgcctcgtc ggcatgctga
     2041 cttcagctca cgtcgccggc gccatgggta tcgaagaccg tgtcgattcg atcatggtgc
     2101 acaattgtac catcattcgc gacacagaac tggttcgtga tgcggctgcg cgcctatcgg
     2161 caagcgactt cgaagcattg ccggttctcg atgcgagcga ccacatcgtc ggtgtcgttt
     2221 cctacaagga catcgtcaag gatctggtcg atgaaccagc ccagaaactg tcaccggaaa
     2281 gtgccgtggt ctatctggcc atgactcgcg accccgaaca ggaggaatac tggctcgaga
     2341 aggtaaccgc caacgggtac cgtgccgcga tcacccaggt cggggccacg gcggaaaagc
     2401 tgccgatcaa actgcgcgaa gcgacaatcg tcgcggccat tgcccggtca gtcattcggg
     2461 aagatccgcg cgagaagttc gcggtcagca atgccgtgcg ggacatctac agtcaatgca
     2521 acgtgatcaa tccgggtctt ggtggcggtt tcaaggtcgc catcgttcgc ggcgaaggtc
     2581 gcatcgcggt ggccgccttc gggcgttgcg gccatgctct ggccaacggt aacgaacaaa
     2641 tcttcatggg agggaatatc atctagatga gctcccccat tcgcgtgcgt tttgccccgt
     2701 ctccaactgg ttatctgcat gtcggcggtg ctcgcaccgc gctgttcaac tggctgctgg
     2761 cccgacagac cggcggcgtt ttcattcttc gcatcgaaga cacggatctc gagcgttcga
     2821 ccgcggaatc ggaagaaggc ctgctgcgtg acctgcgctg gctcgggctg gactggaacg
     2881 aaggtcccgg cgtcggtggc ccgcatggtc cctatcgtca gagtgaacgc accacgattt
     2941 accaggatgc ggcacgccgg ctgatggcca agggggccgc ttatccctgt ttctgctccg
     3001 aggagagcct cgaagccaag cgcaagcagg ccgaagccag tggagccgcc atgcattacg
     3061 acggcacctg cacccgatta ccagccgcag aggttcagcg acgcatatcg gccggcgaac
     3121 cctacgccat tcgcatgaaa gtaccagaga aagattacgt tctcgacgat ctctgccgag
     3181 gttccgttga gtggaagtca gagaatctgg gagatttcat cattcttcgt tcgaatggta
     3241 tgccggtcta caacttctgc gtcgtggtcg atgatgccga gatggaagtc acgcacgtga
     3301 ttcgcggcga cgatcatttg acgaatacgc atcggcagtt gatcatttac gaggccctgg
     3361 agcgccctcg accaaagttc gcgcacgtgt cgatgattct cgggccggac aaagccaagt
     3421 tatcgaagcg tcacggcacg acgtcggtcg gacagtatgc cgaagacggt tttctccccg
     3481 aggccatggt gaacttcttt gcgctgctcg gctggaacga aggaaccaat caggagttgt
     3541 actctcgcga cgaactcatt cagaagttct cgatcgaacg catcgggaac agcgccgccg
     3601 tattcgacca cgcgaaactc ctgtggatga acggtgagca cattcgcaaa ctgtccctcg
     3661 acaccctgac gcagctgctt gaacctcgct tcaagaaagc atttccggga gatgtgcgtt
     3721 tcgacgaccc ggcttttcgt cagcgcgcga cgcaggtcat tcagccggcg ctcaagacgc
     3781 tcaacgatgt cgggcagatt ctcacgccga ttcttcacgg cggcacaccc gaaaacgccg
     3841 aagccgaaga ggctttgaaa ggtccggacg tgccgcgcct gctcgaagct ctgcagccgg
     3901 ttttcggcga aatcgagtgg aagaaggaga tcatcaacca gaccatcaag gatctggcca
     3961 aaaaactgaa catcaaagga aaagctctat tcatgccgat tcgcgtgcgc ctgaccggca
     4021 actgtcatgg cccggatctg gtcggcatca tagaactgct ggggcgagag acggttctcc
     4081 aacgactgaa gccctgatcc tcccaggaca ctaacgagag aagcacccgt gaccgggtgc
     4141 ttcttttgtc cagtcccggt gcgctcgaac tcacctttcg cgaagcggcg tcactgatga
     4201 cgccgtttct gtcgcgaggg actgaggccg tcccagtgcc caatcctgac tggcaattgt
     4261 ctccgccaga gatcgttcat cgatgaagcc gttccatttt cgcaactggg acaattcctg
     4321 aaggacttca ctgccccccg ggtacttttt catgttcacc agcaggtcgc gaatctgctc
     4381 cttgaatgtc gcatccatat cacgacgcac cgccacggga tcattcagca accattcggt
     4441 gaaggcgatg cagaacaggc cttcatgagc gaaacctgag ccgggaacca gccgggtatg
     4501 gcttgcctcc atcaccacgg gcgattcaga cgacacggga accggtatgg ttttttgcgt
     4561 cttcaggtcg agcgcccaca aggcatgacc ggaatacaca tcgtatacag ccgcggcatc
     4621 ataggcccct gacagaaccc ccgagatact ggtggaatga tttccggtga aggtcacttc
     4681 gctgaagaac cgcagaggat cgattccctc agcgcgaaac agttgattcg gcatgacaaa
     4741 acccgttcct gaatgccggt cgacataggc aaaacggcga ccacgcagtt cggccagcgt
     4801 acgaattcca gagccctcgc gaacaacgat cgccccacga tgcctgaccg ctccctcccg
     4861 caatggtcgg caaatggatt cccagcggtt ttctcgattc agtgattcga aagaattggc
     4921 agaaaaccac gccagctgaa cttttccgag atccagtaag cgaccgagtg attcgtaatc
     4981 caccgcaaag ttcagctgga cttcgcgatg caggtttttc gacagatacc cggtcagcgc
     5041 atgaaattcc cgtttcagca actgctccgg caggtatggg atgaccccca tgatcaaagg
     5101 aggagctgac gcaatttccc aggtgatccg aatcgcttcc ggatcactcg tccgattcca
     5161 ccagaagccg atggtcaccg ccgccagcac gacaaagacg atcagaagcc aggttccgcg
     5221 agccatgtac gaacctcctc ttccaggtgt gcctggtcaa gttcctgcag atcgatcccg
     5281 gctgtcgagg ccaaacgcgc ctgccgcgtc atcggatcac cgggttccag taagggaaac
     5341 aggtgcgccg caatggttcg tcccaattgt tcttcgagga actcgagggc caccgagtgc
     5401 acaaatcgat cgctcgtcgt ggctttcacg agcgcttcga gcgtttctgc cggctgtttt
     5461 cccgccagcg ccgtaaagtg aatcaccacg cgcaatcggt tgtccaacca tttgtccagg
     5521 ttattctttc tttgttcatc cttccagaga ccgacagagc atcctcgttc atagatacac
     5581 atctctcgcg ccagtcgttg cagcatgaca aggagtacac gttcataccc ggcatgtcca
     5641 gggccgcaca gaggaatgat gagatctccc caggtcggga accgatcaag atcctgcaga
     5701 agcagacgtt ccaacagggg ttccggatcc agtcggatct ccgttccctc cagacacaga
     5761 cagtttgcca gtaagtcccg atctgcatcc tcgcttccca gagcccgcag caacgtttcc
     5821 cgtttttgcg actcggacac tcgcaaaacc cgcatcaaca gttcgtcatc cgccgacgac
     5881 tgaactgcct tcaaccagaa gccgattctc gaacgttctg ccgcgctcaa acgcgccagc
     5941 accttcgcac gttcttccat cccttcacgg ttcaacagtt cccaggctga ctgagccgct
     6001 gccgtgtgac gccccatgat ccgtttttcc gccagcaatc gtaccgcctg gctgctgccg
     6061 attcgagcca gtgatcgaca ggcatgcctg gaaacttccg cttgttcgtc gtctgcaagc
     6121 actcccagtg cgggaacaaa gacaggcaac ccggtggctc ccatcacgac cgctgccgtg
     6181 gcgcgttcga ccgacagcgt tgaagaaagc aacgacgcca ggtgctcaag acacttcgtc
     6241 agcaccggtc gctggtcgca acgccgcaac aaagtctgca ccgcactgcc acgcactcga
     6301 tggtgcgaat gatcaagaaa ccgttgcagg atctgggtca tgcgctccgt gtcgccggca
     6361 tgcagggttt cgatgatatc ggccaccagt ctcggatcct gttcatgcag cagcagatct
     6421 tcgatcgagt agg
//
LOCUS       DAORHD010000077         6388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12127_length_6388_cov_4.287226, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000077 DAORHD010000000
VERSION     DAORHD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6388
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12127_length_6388_cov_4.287226"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PKO06_02790"
     CDS             <1..1058
                     /locus_tag="PKO06_02790"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNV68598.1"
                     /translation="AIGEMKTKPTQHSVREMRAIGLQPDVIVCRTARPLTDDARDKIS
                     LFCNIEKNHVLEARDVDCLYEIPLAMEEQGLADLVLQRFRLGSPKPDLVEWKGIVSNF
                     KQKKARKVRIALVGKYIKIADAYLSVIESITHASLAHDLGLELVWVSSEQVEKEGAEK
                     VLADIHGVIVPGGFGDRGIEGKIQAIRYCRENQVPLLGICLGMQCVVIEFARHVAGMD
                     DAHSAEFMPDGKHSVVSLMPDQDGLMKGGTMRLGTYPCRIDEKTLAHRLYGTTLIYER
                     HRHRFEVNNDFRARLARKGLMMSGTSPDLRLVEMVEIENHPYFIACQFHPEFKSRPGR
                     PHPLFDGLFKAAAARCS"
     gene            1087..1905
                     /gene="kdsA"
                     /locus_tag="PKO06_02795"
     CDS             1087..1905
                     /gene="kdsA"
                     /locus_tag="PKO06_02795"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNV68599.1"
                     /translation="MRSAFSLKSWRADAPFFVIAGPCVLEMLPEALQIARAVKDIGQR
                     LGLPVVFKASFDKANRTSGAAHRGPGMEAGLRIFQQIEADTGLPMLVDVHWPEQTGDA
                     ARVCQILQIPAFLCRQTDLVLAAAQACRCVNVKKGQFLSPTDTEHIVKKIKSVSPENE
                     VVLTERGTSFGYGNLVVDMRSFPIMRQWADLVVYDATHSLQLPGAGDGKTAGQREYIP
                     FLAQAAMATGCVDGLFLEVHPEPSRSPSDAENIWPLDQLEPLLKRLLDVRRAVG"
     gene            2154..3137
                     /locus_tag="PKO06_02800"
     CDS             2154..3137
                     /locus_tag="PKO06_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNV68600.1"
                     /translation="MNHGPIASPEVILNAARQVLQQEGEAVLAVSRRLDQRVNRAVEI
                     VLNAPGRVIVSGMGKSGHVGRKIAATLASTGTPAFFLHPSEALHGDLGMVTEQDVLLL
                     LSNSGETEEVVRLIPFLRRIGARILAITGQLESTLAKAADVVFDGGVEREACPLNLAP
                     TSSTTVALALGDALAVALLEARGFRERDFARLHPSGSLGRKFLTVRDLMHVGDQMPMA
                     GRGIPLREAIITMSRKSFGALLIADDRHRLLGIFTDGDLRRYFERSDGSLHVPLEKIM
                     IRHPKHIGPDRLAAEALKIMESKSITALPVVEGEVLVGFLHLHDILRARLV"
     gene            3173..3961
                     /locus_tag="PKO06_02805"
     CDS             3173..3961
                     /locus_tag="PKO06_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNV68601.1"
                     /translation="MLDFHTHTFFSNDSRSDPAAMIDAARRRGVRYLAFTDHADFAPT
                     DFRIDPDAYFEQLGQWRGMHDGVEVFVGIEIGWQISHTLEVRSFLERIRRHACDVVIA
                     SFHRSAGLDIGDGTYMRGKTLHDWWRGLLEEMLSAVQAEPDFDILGHLDMMRRYPPAK
                     GSRIPEDLYGMLDRLLGWLAEHDKTLECNSSGFRYGLDGPHPEEWVFKRFRELGGRRV
                     SLGSDAHVPDDVGYWTTEARKMLQRCGFSDVCGFRARVPFLIPL"
     gene            3978..5081
                     /gene="nagA"
                     /locus_tag="PKO06_02810"
     CDS             3978..5081
                     /gene="nagA"
                     /locus_tag="PKO06_02810"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNV68602.1"
                     /translation="MTLQITSTMPDFLDTLRVYDLELTDRPMADSPDGFRLPGMIDLH
                     IHGAYGFDFTHGGPDRIEATLDRLIECGLTGIVPTLITCPEGARLQALEDLKTVALRR
                     RRPPRILGIYLEGPFLSPHRCGSHPPPLLMKPDLEALKRWQNAAGGLIKMVTVAPELD
                     GAIEFMAAARRMGVIPAIGHTDADHETTCRACPEGLGHVTHLYNAMRPFSHREPSVLS
                     ALFSQPRVTVELIGDGIHVAPEIVAMTYRIFGARRISLISDGICPLGMPNGTYDAYGQ
                     KLIMNDGRCALESGKLFGGGLPLIDSLRILSRRAGLSLRDLALSVGFSPAAALGIELP
                     AGDVILDRDLRWLATHFDGKWYTARAATVAEAQ"
     gene            5078..5962
                     /locus_tag="PKO06_02815"
     CDS             5078..5962
                     /locus_tag="PKO06_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68603.1"
                     /translation="MRAAAGDRMWRTTTVLLAFVVWCAVVVHGQAAPWGERMRTYGDS
                     LKDWAGSTTHDLKDWAGSATHDLKDWAGSTTRDLLQQAEGGNCELCGSKTVRGMSRCL
                     RCLHKTAPAEFKQWCDDTWRSTRETVETLRDRRHYQRAIDLLFSARDAVQSQRNSDSQ
                     LVLERNRQWYEQMTRLIMSRDNGEGGRLAREAVKRFAPSLEGTDFVEDPARAFSYFWA
                     LDGKGFVKRVRWLRGPGGKIVTMQEYLEQKTGADPQKSEDVVEIIDDVRKIATEDTRE
                     MAPAALDALARTLRLLTR"
     gene            5974..>6388
                     /locus_tag="PKO06_02820"
     CDS             5974..>6388
                     /locus_tag="PKO06_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNV68604.1"
                     /translation="MALESLPSPAWEESRSRESGEESESESPGIAWQFWVMLLVSLCM
                     VTGVTLLTRGSVQREAEHEFEAAGNEIQAIILYRLSEHARILWSGSAFFDASNQVSRR
                     EWHDFVARQRLNEVLPGIQGVGYSLLIPRADLASHQ"
BASE COUNT         1236 a         1783 c         2011 g         1358 t
ORIGIN      
        1 aagccatcgg cgagatgaag accaagccga cgcagcactc ggtgcgcgag atgcgggcga
       61 tcggtctgca gcccgatgtc atcgtctgcc gcaccgctcg accactgacc gatgatgccc
      121 gcgacaagat ttcgctcttc tgtaacattg aaaagaacca cgttctcgaa gcacgcgacg
      181 tggactgcct gtatgagatt cccctggcca tggaggaaca ggggctggct gacctggtgc
      241 tgcagcgctt tcgcctcggt tcgcccaagc ccgatctggt cgaatggaag ggcatcgtca
      301 gcaacttcaa gcagaagaaa gcccgcaagg tgcgtatcgc cctggtgggg aagtacatca
      361 agatcgccga tgcctatcta agtgtgatcg agtcgatcac ccatgcctca ctggcgcacg
      421 atctcggact cgagcttgtc tgggtttcct cggaacaggt cgagaaagaa ggggccgaaa
      481 aagtcctggc tgatattcat ggagtgatcg tcccaggcgg ttttggtgac cgaggaatcg
      541 aagggaagat tcaggcgatc agatactgcc gcgagaatca ggttccgttg ctgggcatct
      601 gcctggggat gcagtgcgtc gtcatcgagt ttgcgcgcca tgtcgccggg atggatgacg
      661 cgcattcggc ggagttcatg cctgacggca aacattcggt ggtcagcctg atgcctgatc
      721 aggatggact gatgaaaggc ggaacgatgc ggctggggac ctatccgtgc cggatcgatg
      781 aaaagacgct ggctcaccga ctgtacggta cgactctcat ctatgagcgt catcggcacc
      841 gcttcgaagt taacaacgac tttcgcgccc gcctggcgcg caagggtctg atgatgagcg
      901 gcacgagccc tgatctgcgg ctggtcgaaa tggtcgagat cgagaatcat ccctatttca
      961 tcgcctgtca gtttcatccc gagttcaaat cgagaccagg gcgcccgcat ccgctgttcg
     1021 atgggctgtt caaagccgcg gcggcgcggt gttcctgagc gaccaaggag cgacacggtg
     1081 aaccagttgc gttccgcgtt ctcactgaaa tcctggcgtg ccgacgcccc cttctttgtc
     1141 attgccggcc cctgtgtgct ggaaatgctt cccgaggcgt tgcagatcgc gcgtgcggtc
     1201 aaggatatcg gccaacgtct ggggctgccg gtcgtgttca aagccagttt cgacaaggcg
     1261 aaccgcacgt ccggagccgc gcaccgtggg ccgggaatgg aggctggtct caggatcttt
     1321 cagcagatcg aggctgatac cggcctgccg atgctggtcg acgtgcactg gcccgagcag
     1381 accggagacg cggcgcgggt ctgtcagatt ctgcagattc cggcctttct ctgccgacag
     1441 accgatctgg tgctggctgc ggcgcaggcg tgccggtgtg tcaacgtgaa gaaagggcag
     1501 ttcctgtcgc caacggatac ggaacacatt gtcaagaaga tcaagagcgt gagtccggaa
     1561 aatgaagtag tactgacgga acgaggaacc agtttcggat acggcaacct tgttgtcgac
     1621 atgcggagtt ttccgatcat gcgtcagtgg gccgatctgg tggtctatga tgccacgcac
     1681 tcgctgcagt tgccgggggc cggggatgga aaaacagccg gtcagcgcga gtacatcccg
     1741 tttctggcgc aggcggcgat ggccaccggt tgtgtcgatg gtctctttct cgaagtacac
     1801 cccgaaccgt cgcgttcgcc ttccgatgct gagaacatct ggccgctcga tcagctcgag
     1861 cctctgctga agcgattgct cgatgttcgc cgggcggtcg ggtgagttcg agcaaacacc
     1921 cccctgacct gccactctgt cgtcatttgg ccgcatcgct tcgcgaatcg acgtgcgatg
     1981 acgagggcat ctcctttccg ccgtagagaa agggttttgt cgagatgaag tgaagcgcgg
     2041 gaatacctcg aatgatctct ttgatggcgc acacccgagt ggtgtcgagc cctgtcatct
     2101 ctgtgatcgc gaacggcgtt gaaacgggct tgtttctacg ctatcatcgt agcatgaacc
     2161 acggaccgat tgcttccccg gaagtcatac tgaatgcggc acggcaggtg ctgcagcagg
     2221 aaggcgaagc cgtgctggcg gtttcgcgac ggcttgacca gcgcgtgaac cgggcggtcg
     2281 agattgtcct gaacgctccc ggacgcgtga tcgtgtcggg catgggaaaa tcgggtcatg
     2341 tcgggcgcaa aattgccgcg acactggcaa gtaccgggac ccccgcgttc ttcctgcatc
     2401 ccagcgaagc cctgcatggc gatctgggca tggtgaccga acaggatgtg ttgctgttgc
     2461 tgtcaaacag cggcgaaact gaagaggtcg tgcgattgat tccgtttctg cggcgaatcg
     2521 gtgcccgcat tctggcgatt accggacagc tcgagtcgac gctggcgaaa gcggcggatg
     2581 tcgtctttga cggcggtgtc gagcgggaag cctgcccgtt gaatcttgcc ccgaccagtt
     2641 cgacgacggt cgcgctggcg ctgggtgacg cgctggcggt ggctctgctc gaagcccgtg
     2701 ggtttcgtga acgtgatttt gcgcgcctgc acccctcggg cagcctgggc cgcaagttcc
     2761 tgaccgtgcg tgatctgatg catgtcggag accagatgcc gatggctggc cggggtattc
     2821 ctctgcgcga agccatcatc accatgtcca gaaagagttt cggcgctctg ctgattgccg
     2881 atgaccggca tcgcctgctc ggaatcttca ccgacggcga tctgcgccgg tatttcgaac
     2941 gctcggatgg atcactgcat gtgccgctcg agaagatcat gattcgccat ccgaaacata
     3001 tcggccctga ccgcctggct gcggaagccc tgaaaatcat ggaaagcaag tcgatcacgg
     3061 cgttaccggt ggtcgaggga gaggtgcttg tcgggttcct gcatctgcac gatattttac
     3121 gcgctcgact ggtgtagaaa ccggcgagag ccacgaaagg agagaggatc gggtgctcga
     3181 ttttcatacc cacaccttct tctccaacga ttcgcgttct gaccccgccg cgatgattga
     3241 tgctgcgcgt cgccgcggag tgcgttatct cgcatttacc gatcatgccg attttgcccc
     3301 caccgatttc agaattgatc ctgatgccta cttcgaacaa ctgggacaat ggcgaggcat
     3361 gcacgacgga gttgaagtct tcgtaggcat cgagatcggg tggcagatca gccacacgct
     3421 cgaggtcaga tcatttctcg aacgtattcg ccgccacgcc tgcgatgtcg tcatcgcttc
     3481 gtttcatcgc tctgccggac tcgatatcgg agatggcacg tacatgcgag gaaagacgct
     3541 gcacgactgg tggcgcgggt tgctggaaga aatgctgagc gcggttcagg ccgaacccga
     3601 cttcgacatt ctcggacatc tggatatgat gcgccgatat ccgcctgcca agggttcccg
     3661 cattcccgaa gacttgtacg gaatgcttga tcgtctgctg ggctggctgg cggagcatga
     3721 taaaacgctc gagtgtaaca gctcggggtt tcggtacggc ctcgatggtc cgcaccccga
     3781 agagtgggta ttcaagaggt ttcgcgagtt aggagggcgg cgcgtcagtc tcggttcaga
     3841 tgcgcacgtt cccgatgatg tgggttactg gacgaccgaa gcgcggaaga tgctccaacg
     3901 ttgcggtttt tctgatgtct gcggtttccg ggcccgggta ccatttctga ttcctttgtg
     3961 agaaagcgag aagcaccatg accttgcaga tcacctccac catgccggat tttctcgata
     4021 ccttgcgcgt ctacgatctg gaactgactg atcgccccat ggcagacagc cccgatgggt
     4081 tccgtctgcc cggcatgatc gatcttcaca ttcacggcgc gtatgggttt gatttcaccc
     4141 acggcggccc cgatcgcatc gaggcaaccc tggacagact gatcgaatgc ggtttgacag
     4201 gcatcgtgcc cacgctgatc acctgcccgg agggagctcg tctgcaggcg ctcgaggacc
     4261 tgaaaaccgt ggcgctgcgt cgtcgtcgcc cgcctcgcat tcttggaatc tatctcgaag
     4321 ggccttttct gtcgccgcat cgctgtgggt cgcacccgcc gccactgttg atgaaaccag
     4381 atctcgaggc cttgaagcgc tggcagaacg ccgcgggtgg cctgatcaaa atggtgaccg
     4441 tcgctcccga actcgatgga gccatcgagt ttatggctgc cgcgcgccgc atgggggtca
     4501 taccggcaat tggtcacacc gatgccgatc atgaaacaac ctgtcgcgcc tgtccggaag
     4561 ggcttggaca tgtcacgcac ttgtacaatg ccatgcgtcc gttcagtcac cgggaacctt
     4621 cggttctgtc ggcgctgttt tcgcagcccc gcgtcaccgt tgaactcatt ggcgacggaa
     4681 ttcacgtcgc gcccgaaatt gtggccatga cctaccgaat cttcggtgcg cgccgaatct
     4741 cgctgatctc tgatggcatc tgcccgctgg gaatgccgaa cggcacctat gacgcctatg
     4801 gtcagaagct gatcatgaac gacggccggt gtgccctcga atcaggaaaa ctgttcggtg
     4861 gcggactgcc cctgatcgac tcgctgcgga tcctgtcgcg tcgcgccggg ctttctctgc
     4921 gtgacctggc gttgtccgtg ggcttcagtc cggcggccgc gctgggcatt gagttgcctg
     4981 ccggcgatgt tattcttgat cgtgacctgc gctggctggc gactcacttc gatggcaagt
     5041 ggtacaccgc gcgagcggcg acggtcgcgg aggcgcaatg agagcggctg cgggcgatcg
     5101 catgtggcgg acgacaacgg ttcttctcgc gttcgtcgtc tggtgcgctg tcgtcgtgca
     5161 tggacaggcc gcgccatggg gcgaacggat gcgcacctac ggagattcgc tgaaagactg
     5221 ggctggcagc acgacccatg atctcaagga ctgggctggc agtgcgactc acgacctcaa
     5281 ggattgggcg gggagtacga ctcgcgattt gctgcaacag gccgagggag gcaactgcga
     5341 gctgtgcggc agcaagacgg ttcggggaat gtcacgctgt cttcgctgtc tgcacaaaac
     5401 cgcgccggcg gaattcaaac aatggtgcga tgacacgtgg cgatcgactc gtgagacggt
     5461 cgaaacgctg cgtgatcggc gacattatca acgcgctatc gaccttcttt tctctgcgcg
     5521 agatgccgtt cagagtcagc gtaacagcga ttcgcaactc gtactcgaga gaaatcggca
     5581 gtggtatgaa cagatgaccc gtctgatcat gagtcgtgac aatggagaag gggggcgact
     5641 ggctcgcgaa gccgtaaagc gttttgcacc ctcactggaa ggcacggatt tcgtcgaaga
     5701 cccggcgcgg gctttttcct atttctgggc tctcgatggc aaagggtttg tgaaacgggt
     5761 gcgctggctg cgaggcccgg gcggcaagat tgtcacgatg caggaatacc tcgagcagaa
     5821 gacgggtgct gatccgcaga agtcggaaga cgtggttgag atcatcgatg atgtgcgcaa
     5881 aattgcgacc gaagatacgc gggaaatggc tcccgcggct cttgatgccc tggcgcgcac
     5941 gcttcgtctg ctgactcgct gagcggggat tccgtggcgc tcgagtcatt gccgtcacct
     6001 gcgtgggagg aaagccgatc tcgcgaaagt ggagaggaga gcgagagcga atcaccgggt
     6061 attgcctggc aattctgggt catgttgctg gtcagcctgt gtatggtgac cggggtgact
     6121 ttattgaccc gcggcagcgt gcagcgcgaa gccgagcatg agtttgaagc ggcgggcaat
     6181 gagattcagg cgatcatttt gtaccggttg tcggaacatg cacgcatcct ctggagcggt
     6241 tcagctttct ttgatgcctc aaatcaggtc tctcgacgcg agtggcatga ttttgtcgcg
     6301 cgacagcggc tcaatgaggt cctgcccggg atccagggag tcggctactc gctgctcatc
     6361 ccaagggctg atctggcgag ccatcaac
//
LOCUS       DAORHD010000078         6383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12145_length_6383_cov_4.211757, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000078 DAORHD010000000
VERSION     DAORHD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12145_length_6383_cov_4.211757"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2160)
                     /locus_tag="PKO06_02825"
     CDS             complement(<1..2160)
                     /locus_tag="PKO06_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68605.1"
                     /translation="MPSSPLESWPATLDLSTWLEFSLSAQDSITRALALAEIGDRDEI
                     ESYRDHLQNISTGDPDDRCRQIAQSLLARRESLTEARALSSGIEPTPQGVRDLFDRTY
                     PALREALRESVWQASSAGQLDLWRDHLLQEAVPEVVAIGLRLLSRYGTNTDAAFGTTF
                     LNAENPGVVLAAIDLLAVHDTDRLSQYLLFPLHHPSAQVRFHAARFLRATDPDAADRF
                     LNEALTDDDPFVRRLAILELSKQPTESSLTSFVRVIGFETQPLLLVKAGLILTDHPHL
                     DMPPHVYDHLLMSQGLRKHVLNITLRALVQRIRDAGAISLSIEEYLTDLKRELASRRD
                     DLTIALITRDLRHTSIEVRRQAFERCQAFVADPRIQKALRGRRRHESDSELQQVLTHL
                     LQAVPETIQGAPPEASSVPKDNAPVRSAEAIRDFTACVSSGQLYRLPVREQIVQIDAI
                     SYHDLFKSSKGSFFSILQHSTERLVLLRVLKAFERWGSRADAEPLHSLLKRPDPAVIG
                     QTLKTLAHLGDENITTILNPFLREDNLAVKAAALEAFFVCDRSAAGQFLQSMLESPHA
                     LIRRKALALAPLTDHATVESRLIGIMQHDPLPDLQMQAGCLIASNPSDRGLTALFRCC
                     RHPGHAAVPEWQELWNSVRPTAALHFRQSTDHVDAWIEERVRNEEAAQARQTTATAMI
                     ADRAKVRLRNSATRERIENLNLGHSMGEALSLLRVFAL"
     gene            2243..2605
                     /locus_tag="PKO06_02830"
     CDS             2243..2605
                     /locus_tag="PKO06_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68606.1"
                     /translation="MANFDVMCETVDGIPVLRVKGYYEKPAGQKIIEHAEALCQTGKT
                     VLVLDFSVCEVLSSPGVATIVELAADLADNYGGSLIICGLDRLKEKVLMLVGMQTMAR
                     ICASLSDALQAARTAMSR"
     gene            complement(2641..5571)
                     /locus_tag="PKO06_02835"
     CDS             complement(2641..5571)
                     /locus_tag="PKO06_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68607.1"
                     /translation="MWSITRESGTSSPPAPSPWERLRSGLHLVLTLIVFGVLPWGITE
                     LALLTFTDTVKARQIEHLKISQERLLAQVSLLDDETVFASRLINRVFTRLQKHGVEEG
                     RREVARLERLFPHTFELIFFDGSGNLLPEVSSLRRGRTFLERCYTAIGAIFRGEAAEP
                     GQSALLRELFKMPRIESLRQYGQGKPMPIGEDPRAGYCSFHWAKPASSSSIVGASGYL
                     AFYNPDTFAPNTALLHTIAAAEKRIRAMRFGAIDLSRRTPVLTPSSLGSQPSLASALL
                     KASTGYQRTWLDERFLITLVPRKRHGSLFCVQEVPTPLSRTFIRGIRLLLLMWSLFLL
                     HQVFSGRFLVGSRITLRIVSLFLFSVGLPTALLLVGGYRALEDHRHVLLDSLERTMRT
                     RLRQFDEQFPSMIAEIEDRLQKLKREAESHADRTQRINTFTKLKNVRSITDFLLVDGH
                     GRSLWDLREPENAAVAQRYQVGRLMAREIMKRLNGVDQIDVGTLVTESVNSTMSSLAD
                     AGSWELFQRNLGRFTPFGLANEGCFMLFDAVRGKSGQVEQMLCSVMFRAQVESRFFRD
                     HRRALERQTDLNWRVSLWSRTRLMRSIYSEKRSRGDVDEIGLQVHSQQSSIRKIIHTR
                     KGAELIIGQLGQNLQSFVLVASAPLAPLEKQIALLWQWTAALASLLVLATFVIGRLLS
                     EQLLRPIGSINEGIQAIRERDFQHQVPVHTRDELGDVSALMNHVLEEMRDLSIARAIQ
                     ETLFPPGEIRMNRLIVYGQSRTLSDVGGDYVDYVQLDDHRLFGLVGDVSGHGVSAALI
                     MGMAKCFFTIADRHQTPLPELLAGFNRYLYQTIKRKKMMTMILFRWNGETRTLEFANG
                     GHVSPFLQRASTGVVEELAMPCMPLGVRLNATFASREVVLETNDAVMLCTDGLAEAKN
                     PAGEDVGYQTPPVWFGELGGQEPKAMVESLFTRLDSFRGEAPVNDDVTIICLRVLPSP
                     SPTR"
     gene            5936..>6383
                     /locus_tag="PKO06_02840"
     CDS             5936..>6383
                     /locus_tag="PKO06_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68608.1"
                     /translation="MADQKLLLTSVFKPFGVDDAYGVKENVCELMHNQVTRGQGVFSI
                     RSHNRSFGLTFLAENLQTPTTVLDFPTFEEFERELRTGDYTHIGISFIVPNVDKARRM
                     AETARRLAPRAKLYLGGHGAAIEDLEQLISCDGICRGEGVTWLRQRL"
BASE COUNT         1392 a         1726 c         1976 g         1289 t
ORIGIN      
        1 cagagcgaac actcgcagca gagaaagagc ctcacccatc gaatgtccga gattcagatt
       61 ctcaatgcgc tctctggtcg cgctattccg gagtctgact tttgctctgt ccgcgatcat
      121 cgccgtcgca gtcgtttgtc tggcttgagc cgcctcttca ttcctcaccc gttcttcgat
      181 ccaggcatca acgtgatcgg ttgactgccg aaagtgaagg gcagctgtcg ggcgaacgga
      241 attccacagc tcctgccact cggggaccgc ggcatgaccg gggtggcgac aacagcgaaa
      301 tagagccgtg agcccacgat cactggggtt ggaagcgata aggcagcctg cctgcatctg
      361 gagatccgga agagggtcgt gctgcatgat tccgatcaga cgtgactcga cggtggcatg
      421 atccgtcagg ggagcgagcg cgagcgcttt acgacggatg agagcgtgag gcgattccag
      481 catggactgc agaaactgcc cggcagcgct gcgatcgcag acaaagaagg cctcaagggc
      541 ggcagccttc accgcgaggt tatcctcccg cagaaacggg ttcaggatcg tggtgatgtt
      601 ttcatcacca aggtgggcca gggtcttcag ggtctgacca attacggccg ggtcggggcg
      661 cttcaacaac gaatgcaatg gttcggcatc ggcgcgtgat ccccagcgtt caaatgcttt
      721 cagcacgcgc agcagaacca gtctttccgt ggagtgctgc agaatagaga agaatgaccc
      781 tttggaagac ttgaatagat cgtgatacga gatggcatca atctggacaa tctgttcacg
      841 caccggcaac cggtagagct gaccggaact gacacaagcc gtaaaatctc tgatggcttc
      901 ggcagaacga actggggcgt tgtccttcgg cacggatgaa gcttcgggag gtgcaccttg
      961 aatcgtctca ggaacggcct gcagcaggtg ggtgagaacc tgctgcagtt cagagtcgga
     1021 ctcatgccgt cggcgcccgc gcagggcttt ctgaatacgc gggtcggcaa cgaaggcctg
     1081 gcagcgctcg aatgcctgac ggcgaacctc gatcgacgtg tgacgaagat cgcgagtgat
     1141 cagggcgatg gtcaggtcgt cccgtcggct ggccagttcg cgtttgagat cggtaagata
     1201 ctcttcgatg gaaagagaga tcgcgcctgc gtctcgaatc cgctgcacga gggctcgaag
     1261 agtgatattg aggacgtgct ttcgcagacc ctgcgacatc agcaggtgat catacacgtg
     1321 cgggggcatg tcgagatgtg gatgatcagt gagaataaga cctgctttca cgagcaagag
     1381 gggctgcgtt tcaaacccga tgactcggac gaatgaagtc agactgctct cggtgggctg
     1441 tttgctcaac tcgagaattg ccagtcgacg aacgaacgga tcatcatcgg tgagggcctc
     1501 gttcagaaag cgatcagcgg cgtctggatc ggtcgctctc agaaatcgcg ctgcatgaaa
     1561 gcgaacctgg gcggatgggt ggtgcagcgg aaacagaaga tattgcgaaa gtctgtcggt
     1621 atcgtgcact gccagaagat cgatggcggc caggaccaca ccggggttct cggcattcag
     1681 aaacgtggta ccgaatgcgg cgtcggtgtt cgttccgtac cgggaaagca atctcaagcc
     1741 gatggccacg acttcgggaa ccgcttcctg aagcaggtgg tcgcgccaga gatcgagctg
     1801 tccggcagag gaggcctgcc agacggactc acgcagggcc tctcgaagcg cgggataagt
     1861 tcggtcgaac agatcgcgca ctccctgtgg cgtcggctcg atacctgacg agagcgctcg
     1921 ggcttcggtg aggctctcac ggcgggcgag cagactctgc gcgatctggc ggcaacggtc
     1981 gtctggatcc ccggtagaaa tgttctgcag atgatcacgg tatgattcga tctcatctcg
     2041 atcgccgatt tcggccaggg caagggcgcg tgtgatcgaa tcctgcgcgc taagcgaaaa
     2101 ttcgagccac gtcgacagat cgagggtggc cggccaggat tccaggggcg atgaaggcat
     2161 accagacaac gtatcacatc cttcgcgtgc ggtgacaaca gatccgtgta tactgaagaa
     2221 aaccgttcca ggagattccg agatggccaa ttttgatgtc atgtgtgaaa cggttgacgg
     2281 cataccggtt ctgcgagtga agggatacta cgagaagcca gcggggcaga aaatcatcga
     2341 gcacgcggag gcactgtgtc agacgggaaa aacggtgcta gttctcgact tctccgtgtg
     2401 cgaggtgctg agcagcccgg gagtggccac catcgttgaa cttgccgcgg atctggcgga
     2461 taactacggc ggctctttga ttatctgcgg gcttgaccga ctgaaggaaa aggttctcat
     2521 gctggttggg atgcagacga tggcccgcat ctgcgcgtct ctctccgacg ctctccaggc
     2581 cgctcgaaca gcaatgagtc gttgagcgac gagccgatga ggcggcagcg gtcgggcaga
     2641 tcaccgtgtc ggtgatggtg agggcaaaac ccgcaggcag atgatcgtga catcatcgtt
     2701 cacaggagcc tcgcctcgga acgaatcaag tcgggtaaag agactctcga ccatggcttt
     2761 gggttcctga ccgccgagtt ccccgaacca gacgggtggg gtctggtagc cgacatcctc
     2821 ccctgcgggg ttcttggcct cggccagtcc atcggtacag agcatcacgg catcgttggt
     2881 ctcgagtact acctcgcgcg aagcgaatgt tgcgttcaat cttactccca gtggcatgca
     2941 tggcatcgcg agttcttcga cgactcccgt agaggcgcgc tgcaggaagg gcgacacgtg
     3001 ccccccgtta gcgaactcca gggtgcgtgt ttcgccgttc cagcgaaaga ggatcatggt
     3061 catcattttc ttccgcttga tggtttgata cagatagcgg ttgaaaccgg ccagcagttc
     3121 cggcagggga gtctggtgcc gatcggcaat cgtgaagaag cacttggcca ttcccatgat
     3181 cagggccgca ctcacgccat gccccgaaac atcgccgacg agaccgaaca gtcggtggtc
     3241 gtcgagttgt acgtagtcga cgtagtcgcc tccgacatcg gagagcgtcc gactttgtcc
     3301 atacacaatc aatctgttca tgcgaatctc gccgggagga aacaacgtct cctggatggc
     3361 gcgggcaatg gacagatcgc gcatctcttc cagtacgtga ttcatgagtg ccgacacgtc
     3421 acccagctca tcgcgcgtgt gcaccggaac ctggtgctgg aaatcacgct cgcgaatggc
     3481 ctgaatccct tcgttgatgc tgccgatggg ccgcaaaagc tgctctgaga gcaggcgacc
     3541 gatgacgaat gttgccagga caagcaggct ggctagggcg gcagtccact gccagagtag
     3601 agcgatctgc ttctcgagcg gagccagtgg agcggaggcg accaggacga aactttgcag
     3661 attttgtcca agttgaccaa tgatcagttc agcacccttc ctggtgtgga tgatcttgcg
     3721 aatggatgac tgttgcgagt gcacctggag cccgatctcg tcgacgtccc cgcgtgaccg
     3781 cttttccgag tagatcgagc gcatcaggcg ggttctcgac cataggctga cacgccagtt
     3841 cagatcggtc tgtcgttcga gggcgcgacg atgatcgcga aaaaaccggg attcaacctg
     3901 agctctgaac atgacggaac agagcatctg ctctacttgc ccgctcttgc cgcgaacggc
     3961 gtcgaacagc atgaagcagc cttcgtttgc gaggccaaaa ggggtgaagc ggccaaggtt
     4021 ccgctgaaag agttcccagc tgccggcgtc ggccagcgag ctcatggtcg aattgaccga
     4081 ttcggtcacg agggtgccga catctatctg atcgactccg ttcaaacgct tcatgatctc
     4141 acgcgccatc aggcgaccga cctgataccg ctgcgcgacc gctgcgtttt ccggttcccg
     4201 taagtcccag agcgagcgac catgaccgtc gacgagcagg aagtcggtga tcgagcgaac
     4261 gttcttcagt ttggtaaacg tgttgatacg ctgagtgcga tctgcgtggc tttcagcttc
     4321 ccgcttgagt ttttgcagac gatcttcgat ttctgcgatc atgctcggaa actgctcatc
     4381 gaactggcga agtcgtgtgc gcatggttcg ttcgagagaa tccagaagca cgtggcggtg
     4441 atcttccagc gcccggtaac cacctaccag cagcagtgcg gtcggcaggc cgaccgagaa
     4501 caggaacagg ctgacaatgc gaagagttat tcgcgacccg acgaggaacc tacctgaaaa
     4561 aacctgatgc agcagaaaca gactccacat gagcaggagg agacgtatgc cacgaataaa
     4621 cgttcgagaa aggggcgtgg gaacttcctg aacacagaac agggaaccgt gtctctttcg
     4681 cgggaccagg gtaatcagga agcgctcatc gagccaggtg cgctgatatc ccgtcgatgc
     4741 cttcagcaga gcggaggcga ggctgggttg tgacccgagg ctcgacggcg tgagaacagg
     4801 cgtgcgcctt gacaggtcga tcgctccaaa acgcattgcc cgaatacgct tttcggcggc
     4861 ggcaatggta tggagcaggg cggtgttcgg cgcaaaagtg tccgggttgt agaaggcgag
     4921 ataccccgat gctcccacga tactcgaaga actggcgggt ttcgcccagt gaaaactgca
     4981 gtatccggct cgcgggtctt ccccgatcgg catgggtttt ccctgtccat attggcgcag
     5041 cgattcgatg cggggcatct tgaacaactc gcgcaacagt gcagactgtc cgggttcggc
     5101 ggcttcaccg cgaaagatcg caccgatagc cgtgtagcac cgttcgagaa acgtacgacc
     5161 gcgtcgaagc gagctgacct cgggcagaag gtttccggag ccgtcgaaga aaatcagttc
     5221 gaaggtgtgc ggaaaaagtc tctcgagacg cgcgacttcc ctgcgacctt cctcgactcc
     5281 atgtttctga aggcgcgtga agacacgatt gatcaaacgg gaggcaaata cggtttcgtc
     5341 gtcaagcagg ctgacctgtg cgagaagccg ttcttgcgag attttcagat gttcgatctg
     5401 tcgagctttg actgtgtcgg tgaaggtgag aagagcgagt tcggtgattc cccaggggag
     5461 aaccccaaag acaatcaaag tgagcaccag gtggagaccc gaacgcagtc tttcccacgg
     5521 gcttggcgca gggggtgagc tggtaccgga ttctcgggtg attgaccaca tgacgagatt
     5581 tcacgatacc acaggcagga cgcagagggc gtgacagcgg acggtgcggc gatcccgcat
     5641 tgatcatcgt gtgatttcaa gaccagcagc tctcttcgag agcttgatgt tgcaccacga
     5701 tactgcgaaa acacccgaat tcgtctgcag atgtgcttcg cgctcaccct cttccgtagc
     5761 gggttgccag ggtggcatcc cgcaaaatgt actgcaaatt gtcatgcagt gacagttcga
     5821 gttgctgagg ggcagtggtc gcgcttgacg ccgattgtcc gctgttctcc ctgttgtgat
     5881 cggtttctcc agccacgtga tctcaatccg tgctatcatg cccgcgaggt gagacgtggc
     5941 tgatcagaaa ctgttgctga cgtctgtgtt caagcccttt ggggtcgacg atgcctacgg
     6001 ggtgaaagag aacgtctgcg aactcatgca caaccaggtg acccgcgggc agggcgtgtt
     6061 ctcgattcgc tcgcacaatc gcagtttcgg gctgaccttc ctggccgaga acctgcagac
     6121 tccgacaacg gtgctcgatt ttccgacgtt cgaggagttc gagcgggagc tgcgaaccgg
     6181 cgattacacc cacatcggca tctcgtttat cgtgccgaat gtcgacaaag ccagaaggat
     6241 ggccgagacc gcgcggcgac tcgcacctcg ggccaagttg tatctcggcg gtcacggcgc
     6301 tgcaatcgaa gatcttgaac agttgatttc ctgtgacggc atctgccgcg gtgaaggcgt
     6361 gacctggcta cgacagcggc tgg
//
LOCUS       DAORHD010000079         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12158_length_6378_cov_3.040171, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000079 DAORHD010000000
VERSION     DAORHD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12158_length_6378_cov_3.040171"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..626)
                     /locus_tag="PKO06_02845"
     CDS             complement(114..626)
                     /locus_tag="PKO06_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68609.1"
                     /translation="MFQAFNRKVRGFTLTEIIIGMAILIIIFSIVMRWFLMQRGYQQR
                     ITQIADTQDRFRLAVWNMVQELQTGRVIIWPRLNSDKSIRSDTKVVFKNMLGEIVTFY
                     HIPEALEIRRCVIPPQPGLTPQLDERVVGTGIASVSFTARDIGNRLIDIHLQASGVHT
                     LDAVYLLNED"
     gene            complement(610..1056)
                     /locus_tag="PKO06_02850"
     CDS             complement(610..1056)
                     /locus_tag="PKO06_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68610.1"
                     /translation="MALSILLTCLLPIIHLFGSTSRTAQKSQNLGLAVSLAHRIAQHL
                     YITPFNEIQNISLPGLPIADGSADSLFNPIENWTTSAVGELSIQASQLPSLYSFLRKY
                     EFRYALLVNNVSFGTGDELKSISIIITWKEGGQDLLYKLQTHVPGF"
     gene            complement(1155..2699)
                     /locus_tag="PKO06_02855"
     CDS             complement(1155..2699)
                     /locus_tag="PKO06_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68611.1"
                     /translation="MGNRDTERLPTDGSSNDRDLVQLIECWSSEVGQPTLRPSWGRLK
                     QRLSESREDVEQTYRRLEVSVSSESSLLLILALTQPATALRHQLLRNALLKLIGKPCH
                     RVLGTLLFLPPDEIRENLAPLVYSRGVQGQRAAYLMARLNLSLNAEAVRAILDLEIHD
                     RSFLINLSHLVEPAVRLSLRQSLLQDQPPPTGSPPAAQIDTFCEYLLDGPPGDPISPD
                     ATSTVPSNETPGTPEPSSGTSLVTPEPIAQSPGDTVPVTQIAQRLEKTVPVTSSVPLC
                     ATGSGSSERKEAQSSFLHGDMFGISKRRFWPYFIGISLGLLILGLLVSGSETEGPARY
                     QPLSSQAPAPEYWIDAVSRQRVTRSFLKADSDFQMGEIFANRGDFSSALKLFQDALTE
                     YPAHVGARFREGFCLMQMQDYKGAATSFRKVLAHRADYPLVNLYLGRIALVNFEPDKA
                     AEHLQKEYALRKDLRVGIEYSRLLNQLGKFPESIQILSDLHRRFPTDLAVSSMLTFAQ
                     EQMKGK"
     gene            2846..4057
                     /gene="aroC"
                     /locus_tag="PKO06_02860"
     CDS             2846..4057
                     /gene="aroC"
                     /locus_tag="PKO06_02860"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV68612.1"
                     /translation="MGRLRQLTAGESHGEALIGILDGLVRGIPVSDAAIASQLKRRRV
                     GYGRSTRQSQESDRFRIIGGVRQGMSTGNPFGILLENDSSSPAPETLKSTSRKQSKSA
                     LTKDMCCPRSGHADFVGMLRWGETEAVDVAERASARETAVLVALAVPARELLAQLGVL
                     LGGMVTRMGSIEAQIDATASVSDLLRRTAKTGDVWMTPDSVVVPRWREVVDEARHDGD
                     TLGGAIRVLVDGLIPGLGGFSQRWQRIDGRLSAGLMALPGVKAVSIGRALETSRSRGS
                     ACCDEMRYSDSTGVRRLTNWAGGLEGGMTNGEPLVIELLMKPIPTGKGRMSVNVKTLR
                     RSAAACPRHDTIALAALAVIAESVVAIELADAYLELLGGTTLEDVKAARKRYLARLRP
                     LWLPKRSSRRS"
     gene            complement(4058..5044)
                     /locus_tag="PKO06_02865"
     CDS             complement(4058..5044)
                     /locus_tag="PKO06_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNV68613.1"
                     /translation="MKRIARIIARGSYIPTEEHPNAELRARFGDDIITKSEEATGILT
                     RFYAPKDWAASDLAVKAVEAALADAELKPEQIDLLVLGTDTPDYLTPATSVVVQHKAG
                     LKRAGTFDVGCACASFPTGLAIASGIMATQPNIKRALVVGVYMMHKLADLEKDPMCFY
                     YGDGAGAVVLETGSEPGFIASTFAADGSYATNWGIFAGGTAEPATPAAVNAGRTQVRF
                     VTAFPSTVNREGWPQRVREVAANGGFAVTDIDHIIFTQVRSKTITQVMEDLGLPETKA
                     HKIMQNYGYMGSACLPVAFADAWKLGKIKPGNLVVFVGSGVGYNQCAVAFRV"
     gene            5295..>6378
                     /locus_tag="PKO06_02870"
     CDS             5295..>6378
                     /locus_tag="PKO06_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV68614.1"
                     /translation="MKTSYRVGGVLVLCYLVAWLDRMAINMTIQDMAKDLNIGPERIG
                     WILSAFFAGYALFQIPGGMLADRVGARLVILCALAWWSVFTGFTGLASTFGMMLAVRF
                     FFGVGEGIFPAAVWKVIGSWFTKKNRASANALIISSIALGPALTPLILAPVLGKYGWR
                     TCFYVLGLLGAVCWVVCHRMVYNSIREHPRSSPEEIEEYEADQKSETANREATLQKAS
                     FGDLLKTPIVWVLFSIGLVCNIAMYGWLNWLPSYLLREKNLDLKSMAFAASIPFMFGA
                     IGCMSAGFISDRWFRGRRKLLVLICMGTGLLSLFLFTRVSDMTTAMVMQCVAGLSLFM
                     ASGALWALPMIFLPPALMGSGSGFINT"
BASE COUNT         1317 a         1671 c         1960 g         1430 t
ORIGIN      
        1 ccaggtagag cgcgatgggc acgagaacgg ccatgaagct gatcacgacg atcatcgcca
       61 cgccgtgcga cgaacacctc gtggattggt ggatggggaa aagtgtacgc atctcagtcc
      121 tcattcagta agtagacagc gtcgagcgta tggacgccgc tggcctgcag gtggatatcg
      181 atcagacggt tgccgatgtc acgagcggtg aacgacaccg aggcaatgcc ggtaccgacg
      241 acccgctcgt caagctgcgg cgtcagcccc ggttgcgggg gaatgacaca gcgacggatc
      301 tcgagagcct cgggtatatg gtagaaggtc acgatttcgc ccagcatgtt cttgaatacg
      361 accttcgtat cgctgcgtat ggatttgtcc gagttaaggc gcggccagat gatcactctg
      421 cctgtctgta gctcctgcac catattccag accgccagac ggaatcggtc ctgggtgtcg
      481 gcaatctggg tgatgcgctg ctgatatccg cgctgcatga gaaaccatcg catgacgatg
      541 ctgaaaatga taatgagtat cgccatgccg atgatgatct cggtgagagt aaacccgcgc
      601 acttttctat taaaagcctg gaacatgagt ttggagcttg tacaatagat cctgcccccc
      661 ctccttccag gtgatgatga tcgagatgct tttcagttcg tctccggttc cgaaactgac
      721 gttattcacc agaagagcgt agcgaaattc gtacttgcgc aaaaaagagt acaagctcgg
      781 caactggctc gcctgaattg acagctcgcc gacagcagat gttgtccagt tttcaatcgg
      841 gttgaacagt gagtcggcgc tgccatcggc aatgggcaag cctggcagcg aaatgttctg
      901 aatctcgttg aaaggcgtga tatacaagtg ctgggcgatg cgatgtgcca gactgacggc
      961 caagccaagg ttctggctct tctgcgcggt gcggctggtc gaaccgaaca ggtggatgat
     1021 cggcaaaagg cacgtcaaca gaatgctcag cgccatgacc acctcaagaa acgatgttcc
     1081 ccgttgctcg aatcgagcgt gagatgagtg caggtcacaa ccggccgggc aagtgaaggt
     1141 tccctggttc atcgtcactt gcccttcatc tgttcctgtg cgaatgtcag catcgaggat
     1201 acggccaggt cggtgggaaa tcgacggtgc aggtccgaga gaatctgaat cgactcggga
     1261 aacttgccta actgattcag caggcgactg tattcgatgc cgacgcgcag gtctttgcgc
     1321 agggcgtact ctttctgcag atgttccgcg gctttgtccg gctcgaaatt gaccagggcg
     1381 atgcgaccca gatacaggtt gaccagagga taatcggcac ggtgcgccaa gaccttccgg
     1441 aaggaagtgg cggcgccctt gtaatcctgc atctgcatca ggcagaatcc ttcgcggaag
     1501 cgagcaccga catgcgcagg atactcggtg agtgcatcct gaaacagttt cagcgctgag
     1561 gaaaaatcgc cgcgattggc gaagatctct cccatctgaa agtcgctgtc agccttgaga
     1621 aacgagcgcg tgacccgttg tctggaaacg gcatcgatcc agtattccgg ggccggagcc
     1681 tgactggaga gcggttggta tctggctggc ccttcggttt cgctgcctga gaccaacagc
     1741 ccgagaatca gcagtccgag gcttatgccg atgaaatacg gccagaatcg ccgtttcgag
     1801 atgccgaaca tatccccatg cagaaatgag gattgagcct cttttctttc agaactgccg
     1861 ctgcctgtcg cgcagagtgg tacagatgaa gtgactggaa cagtcttttc aagcctttga
     1921 gcgatttgag taactggtac ggtgtcgccc ggactctgtg caatcggttc gggagtgacg
     1981 agcgaagttc cagacgacgg ttcgggagtg cccggtgtct cgtttgaggg gacggtggag
     2041 gtcgcatcgg gtgagattgg atcgccgggc ggaccgtcga gcagatactc gcaaaacgta
     2101 tcgatctgag ccgccggcgg cgatcctgtc ggtggcggtt ggtcctgcag cagcgactga
     2161 cgtaacgaaa gtcgcacggc aggctcgacg agatggctga gattgatgag gaaactgcga
     2221 tcgtggatct ccagatcaag aatggctctg accgcttcgg cattcaggct gagattgagc
     2281 ctcgccatga gataggcggc tcgctgcccc tgaaccccac gtgagtacac gagcggcgca
     2341 agattctcgc gaatctcgtc gggtggcaga aacagaagag tgcccagcac ccgatggcat
     2401 ggtttgccga tcagtttgag cagggcgttg cgcagcagtt gatggcgtag cgcagtcgcc
     2461 ggctgggtta gggcaagaat caacagtaaa ctgctctctg aagacacaga gacttcgagc
     2521 cgccgatacg tctgctcgac gtcttcgcga gactcggaca gtcgttgttt caggcgtccc
     2581 cagcttggac gaagcgtcgg ttgtccgact tcactcgacc agcactcgat cagttgtacc
     2641 aggtcgcgat cattcgatga accgtctgtc ggcagccgtt ccgtgtctct gtttcccatg
     2701 cgatacgatt gattatttca cagtttttcg ctgccagcaa ctgggcgatg cagagagcag
     2761 gtgatggctc tatgcctgcg cctgatcctg cggtagaatc agagccgtca ggtggcacac
     2821 atgggttgtt caggaggcat tcggcatggg caggttgcgg caattgacgg cgggcgagtc
     2881 acacggtgaa gcactgatcg ggattctgga tgggctggtc aggggaattc cggtcagcga
     2941 cgcggccatt gcctctcaac tgaaacgacg tcgagtgggc tacgggcgca gtacgcggca
     3001 aagccaggag tccgatcggt ttcggatcat tggcggtgtg cgacagggaa tgtccaccgg
     3061 aaatcctttc ggaattcttc tcgagaatga ctcgtcgtcg ccggcaccgg aaactttaaa
     3121 aagtacgagc cgaaaacagt cgaaaagtgc attaacgaag gatatgtgct gccctcgatc
     3181 tgggcatgcg gatttcgtgg gaatgctgcg ctggggcgag accgaggccg tagatgtggc
     3241 ggaacgggca tcggcgcgcg aaacagcggt tctcgtcgcg ctggcggttc cagcgcgtga
     3301 actgctggcg cagcttggcg tgctcctcgg tggcatggtg acccgtatgg ggtccatcga
     3361 ggcccagatc gacgcgaccg cttccgtcag cgatttactg cgccgaaccg cgaagacggg
     3421 tgatgtctgg atgaccccgg actccgttgt tgttccacgg tggcgtgagg tcgtggacga
     3481 ggcccgacac gatggcgaca cgctgggcgg tgcgatacgt gttctggtcg atggcttgat
     3541 tccggggttg ggcgggttct cgcagcgatg gcagcgcatc gatggcagac tgtccgctgg
     3601 acttatggct ctgcccggtg tgaaagccgt gtcgatcggt cgggcgctgg agacgagccg
     3661 atctcgcgga agcgcctgct gtgatgagat gcggtattcc gacagcaccg gcgtgcgtcg
     3721 cctgacgaac tgggccggcg gtctcgaagg tggcatgacc aatggagaac cgctggtgat
     3781 cgaactgctc atgaaaccga ttcccaccgg gaagggacga atgtccgtca atgtgaagac
     3841 gcttcgacgt tcggctgcgg cgtgcccacg acacgacacc attgctctgg cagcgctggc
     3901 ggtgattgcc gaatcggtcg tggccatcga actcgccgat gcgtatctgg aattgttggg
     3961 tggcacaact ctggaagacg tcaaagcggc tcgaaaacgc tatcttgcgc gtcttcggcc
     4021 gctctggctt ccgaaacgat ccagccggag gtcttgatca gacgcggaaa gcgaccgcgc
     4081 actgattgta cccgactccg gaaccgacga ataccaccag attccctggc ttgatcttgc
     4141 cgagtttcca ggcgtcagcg aaagccaccg gcaggcaggc tgagcccatg tagccgtaat
     4201 tctgcatgat tttgtgggct ttggtttccg gcaggccgag gtcttccatg acctgggtga
     4261 tggttttgct acggacctgt gtaaagatga tgtgatcgat atctgtaacc gcgaatccgc
     4321 cattggcagc gacctcgcgt actcgctgtg gccagccttc gcgattgacg gtcgacggaa
     4381 aagccgtcac gaagcgtacc tgggtgcgac cggcattcac cgccgcgggt gtggccggct
     4441 cggcagtacc accggcgaaa attccccagt ttgtggcata ggagccgtct gcggcgaagg
     4501 tcgaggcgat gaatccgggc tctgatccgg tttcgagcac gaccgctccc gcgccatcgc
     4561 cgtagtagaa gcacatcgga tccttctcga ggtcggccag tttatgcatc atgtacacgc
     4621 cgacaaccag agctcgcttg atattgggct gcgtcgccat gattcccgaa gcaatcgcca
     4681 gtccggtggg aaaagaggca caagcgcagc caacgtcaaa agtgcctgca cgtttcaggc
     4741 ccgccttgtg ttgcacgacg acagatgtgg caggggtcag ataatcgggg gtgtcggttc
     4801 caagaaccag gagatcgatc tgctcgggtt tcagttcggc atcggccagt gccgcttcga
     4861 cggctttaac agccagatct gaagcggccc aatctttggg ggcgtaaaac cgggtcagaa
     4921 ttcccgttgc ttcttcactc ttggtgatga tgtcgtcgcc gaatcgtgcg cgaagttctg
     4981 cgttcgggtg ttcttcggtc gggatgtagc tccctcgggc gatgatgcgg gcaatgcgtt
     5041 tcatgacggc tccttgcggg ttgaagtgtg tcagacataa acccgaaaag gggagcctct
     5101 gtcaaggaaa attttgctat gcacaatagc gataatgctc atcaataatg acgaaatccg
     5161 actttggact ccgattcagc tggcaatctg tcctgacgaa agatggctct gaaaaaccaa
     5221 taatggcttg cgcacaggga tgtatgatgc tatatctgtt gccttttaat attgcactgc
     5281 aacatggagg aactgtgaag acgagttatc gagtcggtgg ggtgctggtt ctctgctacc
     5341 tggtggcctg gcttgatcgc atggcgatca acatgacgat tcaggacatg gcgaaagacc
     5401 tcaatatcgg tccagagcga atcgggtgga ttctgtcggc tttttttgcc ggatacgctc
     5461 tgtttcagat tcctggcggc atgcttgccg atcgcgtcgg ggctcgtctc gtcattctct
     5521 gcgcgcttgc ctggtggtct gtcttcacgg gcttcaccgg tcttgcctcg acatttggca
     5581 tgatgcttgc cgtgcgcttc ttcttcggtg tcggcgaagg catttttccg gcagcggtct
     5641 ggaaggtcat cggttcctgg ttcaccaaaa agaatcgggc atcggccaat gcattgatca
     5701 tctcctcgat cgccctggga cccgcactga caccgctgat tctcgcaccg gtgctgggaa
     5761 aatacggatg gcgaacctgt ttttacgttc tgggccttct gggcgcggtt tgctgggtag
     5821 tgtgtcaccg aatggtgtac aattccattc gtgaacatcc gcgaagctct ccggaagaga
     5881 tcgaggaata tgaagccgat caaaagtcgg agacggcgaa ccgtgaagcc acgctgcaga
     5941 aagcaagttt tggagacttg ctgaagaccc cgattgtctg ggtcctcttc tcgatcgggc
     6001 tcgtatgtaa catcgcaatg tacgggtggt tgaactggct cccatcgtat ttactgcgcg
     6061 aaaagaatct cgatctcaag agcatggctt ttgcggcttc catcccattc atgtttgggg
     6121 caatcggctg tatgtcagcc ggcttcatct cggatcgctg gtttcggggt cgacgcaagc
     6181 ttctggttct gatctgcatg gggactggct tgttgagcct gtttctgttt acccgagtca
     6241 gtgacatgac aactgccatg gtcatgcagt gtgtggccgg cctgtcgttg ttcatggctt
     6301 ccggagcgct ctgggcgctg ccgatgatct ttctgccacc tgcgctcatg ggctcaggat
     6361 cagggtttat caataccg
//
LOCUS       DAORHD010000080         6317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12361_length_6317_cov_3.820984, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000080 DAORHD010000000
VERSION     DAORHD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12361_length_6317_cov_3.820984"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(345..797)
                     /locus_tag="PKO06_02875"
     CDS             complement(345..797)
                     /locus_tag="PKO06_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68615.1"
                     /translation="MKKVLFAVLMVMAICLPSFAEEPAPAAAPAADAAKPADAAAPAP
                     AADAKPADAAAPAPAVEKFEGTVVVVKGAEGAADTYTLKTAAAELVLLPGDKLEELKK
                     VADLDKKTFVIEGEKVAGKDGKDGIMIKSFEEKKADAAAPAPATEEKK"
     gene            complement(983..2371)
                     /locus_tag="PKO06_02880"
     CDS             complement(983..2371)
                     /locus_tag="PKO06_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV68616.1"
                     /translation="MNQILLIDDEKLILWGLQRDLERVGFKVSAFQKAEDAFETLEKT
                     HFHAALVDVRMPGIGGLAMLDRIKQVDPETQVIMITAFADVEVAVESIRRGAFDFVLK
                     PFTLEKIRLTIQNALEAVRLKKEVASLKTELARRQHSARVLGLSPRMRLVTEQIERIG
                     QAGAGVVLICGESGTGKGVAAQELHRIGPRSEAPFIEINCGAIPENLFESELFGHEKG
                     SFTGATELKKGLMELADGGTVFLDEVGEMPLPIQTKFLKALEEQRIWRVGGRRPIQLD
                     VNIVAATNRDLTQAVRDGEFREDLFYRLNVVPIFLPPLRERGEDILQLADEFLKHYQN
                     RYRRSFSGFSDQAGKHLLSYPWPGNIRELKNCIERAVILETAESITVESLGIGTTPAH
                     LALPKTNGVPEEPLSDQGIDLIGRLETIETKYLQEALKKCGGNQSQAAKMLGMTRDVL
                     RYRLKKYGLGEG"
     gene            complement(2368..3855)
                     /locus_tag="PKO06_02885"
     CDS             complement(2368..3855)
                     /locus_tag="PKO06_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68617.1"
                     /translation="MRRFGLSQRVILGSILLFLLVTLVMGMTWYRLSRLVEATTHLRG
                     SDDVIVMGSQVQDDLLYLLQTQEFFDQYLSVQTWKHYFELSQRVNKLLNRARSARIYQ
                     RAENELESLDKARREMSNVLENATYSLDPNFPVGAVSPDTLKKIRESRSRLHQQISAL
                     MVKERENRQSLEELLHEQLDSIMETTFWVAVAVLLAGCTFAFYLHRAAVTPIKNLSAT
                     MREAERELTEVVPPPGAAPEMQELVESFNRMTTSLNRHQKRISSMLSLAVTVAHEVRN
                     PIAAIGTAIQAIEKSYPADGADREIFAEILKEVHRVNSIISDLLVFARPRPVQAEYFR
                     ISELVQELQILMHPFLEGKKIGFSCDIPADLDEIEADRNQMHRLLLNLLTNASDAIGD
                     SGQMKIVVESHSDSMIRLVVEDSGPGVPESDREKVFYPFFTTRAKGTGLGLSIVSDIV
                     ERHHGQIRVTDGKLFSGARFEIEMPRRFVADEAAPDQERSERSRS"
     gene            complement(3937..6171)
                     /locus_tag="PKO06_02890"
     CDS             complement(3937..6171)
                     /locus_tag="PKO06_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68618.1"
                     /translation="MISIHVTLPGCEKRGSAIIVVLMVCLVLGIMVGAILNFQRGQAH
                     LLSKSAKDYLALCAADAGLNCVLAEMRANPQFVTHGSIYIPLKGWTSPAKHRPFVVGE
                     TRGLELDHNGNGTYGGRVVLEKTRIIGDFRVRVRLLSAKNSLDTKTVDEAHRYFLLEA
                     VGRVDDSHRKVTAILERFTPTSFLLYDGGILDTGAYGPYRLTPGVLRRGRLYGHEGLK
                     IARRGMIDQGSDLLDMEKISTPGYLEIRQSTRLSFRNGQEGRLDSGNSSSKMDKFKTF
                     PEERGGRVIGHFVLDGMRGAKSERLPPLNPNYWKEATRPKPTMLGPGSSFRGFSASKW
                     RNPAKPQEAVYDLFFGWEYESKKEDMLLYSTVPLRIWGCPPWKALTIFCEKDVYIAGD
                     FNQHPDFPQNYELGYKDYTSQPENGSEKNGCMILSQGRIWFDYSHPIQYLRNELVNLI
                     DYEIAMRLGGKDLNPAALAAICYPPRFSTNSDPRQPMLALHFKGIASLFALPKEPPQV
                     IPVTLAGLVMHSALKELREFLEPGKNAAESATRFGIKSLICREKVLAKIGAACYMTGF
                     LTKGERNSVINDLLDQAEKEDRDEPNPNLGPWNVADRLFRLAVTHPKMGFRFPEMTVN
                     ALLVDSAELNARWDQGDHLDKVLPEIGNITSKEAICFPFVGSDTRMLLRHLGGRVHLR
                     TQPSDVFLDGSAREDFFLVRRNQYDHSLVAGGGDYYPSYVPAGFAVINWQDDSCTREE
                     FDSF"
BASE COUNT         1367 a         1864 c         1711 g         1375 t
ORIGIN      
        1 tggactctgg ttctcccagg cttcgatttc ctgcatctgc tgcagttcat actgttcgcg
       61 aatgggttcc atgtcggcac tccttcagtg attgacgctc agaataccat accaccagca
      121 gaggagacca gcaacacgag cctcagcagc gtctcgcgca gtctcgaccc ccgctgccga
      181 cttccctggg agcggacaca aatcgcggtg ttgcgccatg agtacaagtt atcatctgcc
      241 gcaacgcagt ctgtacaaga tgataacggg agtgagttac cagttcaaat gccgagaaat
      301 gaaaaactcc cgaccgcggg gccgggagtc tctgcgatca gagcttattt cttttcttcg
      361 gtggcgggag ccggagcggc ggcgtcagcc ttcttttctt cgaaggactt gatcatgatg
      421 ccatctttac cgtctttgcc agcgaccttt tcgccttcga tgacgaaggt cttcttgtcg
      481 aggtcggcaa ccttcttcag ctcttccagc ttgtcgccgg ggaggagaac gagctcggcg
      541 gcggcggtct tcagggtgta ggtgtcggca gcgccttcag cgcccttcac gacaacgacg
      601 gtgccttcga acttctcaac ggcgggagcc ggagcggcgg cgtcagcggg cttggcatca
      661 gcggcgggag ccggagcggc ggcgtcggcg ggcttggcgg cgtcagcagc gggagcggcg
      721 gcaggagccg gctcttcagc gaacgaaggg agacaaatcg ccatgaccat cagaacagca
      781 aacaacactt tcttcattgt aaaactcctt ggtgtgattt tatgcgtaac cgcatgactt
      841 caggttgtgc aaagggcgta ccaacctcca aaccaggctt caacaggaga ttgtttcagg
      901 gaatcagtgc ctttctgggg aattcaaccc agaattgtgg gtgaaaaccc ccactttcgg
      961 tccaggtatc gcgttttttt cctcaaccct cgccgagtcc gtatttcttc agacggtagc
     1021 gtagaacatc gcgggtcata ccaagcatct tggcggcctg actctgatta ccgccgcact
     1081 ttttcagcgc ttcctgcaga tacttggttt cgatcgtttc caggcgtcca atcaaatcga
     1141 ttccctggtc agacagaggc tcttcaggaa ccccattcgt tttcggcaaa gccagatgag
     1201 ccggggtggt tccaatgccg agcgattcga ccgtgatcga ttcagccgtt tcgagaatca
     1261 cggcccgctc gatgcagttc ttgagttcgc gaatgttccc cggccacgga taggacaaca
     1321 gatgcttccc ggcttgatcg gaaaaaccgg agaagctgcg tcgatagcgg ttctggtagt
     1381 gtttcaggaa ctcatcagcc agctgcagaa tgtcttcacc tcgttcgcgc aacggcggca
     1441 gaaagatcgg aaccacgttg agccggtaaa acaggtcttc gcggaattcg ccatcgcgca
     1501 ccgcctgtgt cagatcacgg ttcgtggcgg ctacgatgtt gacgtcgagt tgaatcggtc
     1561 gtcgcccgcc gacgcgccag atgcgctgtt cctcgagcgc tttgaggaac ttggtctgaa
     1621 tcggcagcgg catctcgccc acttcatcca ggaagacagt cccaccatcc gccagttcca
     1681 tcaatccctt tttcagttct gttgccccgg taaaggagcc cttttcgtgg ccgaacaact
     1741 cggactcgaa gaggttttct ggaatcgcgc cacagttgat ttcgataaag ggagcctcgg
     1801 aacgtggacc gatccggtgg agttcctgag ccgccactcc tttgcccgtg ccgctctcgc
     1861 cgcagatcag caccaccccg gcccccgcct gcccaatccg ttcgatctgc tcggtcacca
     1921 gccgcatgcg cggcgacaga ccgagcaccc gggcagagtg ctgccttctc gccagttccg
     1981 tcttcagtga ggcgacttcc ttcttcaggc gcaccgcttc aagtgcattc tgaatggtca
     2041 gacgaatctt ctcgagcgtg aacggtttga gcacgaagtc gaaggccccg cggcgaatgg
     2101 actccaccgc cacttcgaca tcggcgaacg cggtgatcat gatcacctgc gtctcggggt
     2161 caacctgttt gatgcgatcg agcatggcga gaccgccgat tccgggcatg cgcacatcga
     2221 ccagagccgc gtgaaaatgc gttttctcga gcgtctcaaa ggcatcttcc gctttctgaa
     2281 aggccgagac cttgaacccg acgcgttcga gatctcgctg caacccccag agaatcagct
     2341 tctcatcatc gatcaacagg atctggttca tgatctgctc cgttcacttc gttcctggtc
     2401 cggggccgcc tcatcagcca caaaacgacg cggcatctcg atctcaaatc gcgctccgga
     2461 aaagagcttg ccatcggtga cccgtatctg tccgtggtga cgctcgacaa tgtccgacac
     2521 gatcgacaat cccagacctg tgcccttcgc gcgagtggtg aaaaacgggt aaaagacctt
     2581 ttcgcggtca gactccggca caccgggtcc cgaatcctca acgaccaggc gaatcatcga
     2641 gtccgagtgc gactcgacca caatcttcat ctggccgctg tctccgatcg catctgacgc
     2701 attcgtcaac agattcagca acaggcgatg catctgattt cgatccgctt cgatttcatc
     2761 aaggtcggca ggaatgtcgc aggaaaaacc gattttcttg ccttcgagaa acgggtgcat
     2821 gaggatctgc aactcctgta caagttctga tatcctgaag tattctgcct gaacgggtct
     2881 tggccgagca aacaccagca aatcggaaat aatactgttg acccggtgaa cctccttcag
     2941 aatctcggcg aagatctctc ggtcagcacc gtcggcgggg tagcttttct cgatggcttg
     3001 aatcgccgtg ccgatggccg caatcggatt tcgcacctca tgcgcaaccg tcaccgccag
     3061 ggacaacatg gacgagatac gcttttgatg acggttgagc gaggtcgtca tgcgattgaa
     3121 cgattcgacc agttcctgca tctccggggc ggcacccggg ggaggcacca cctcggtcag
     3181 ctcgcgttcg gcttcgcgca tggttgccga gagattcttg atcggtgtca ccgccgcccg
     3241 atgcagatag aacgcgaagg tacaccctgc cagcagcacc gccacagcaa cccagaaggt
     3301 ggtttccatg atcgaatcga gctgctcatg aagcagttct tcaagtgact gccggttctc
     3361 gcgctctttg accatcaggg cactgatctg ctggtgcaat cgtgatcgtg attcccgaat
     3421 ctttttcagc gtgtcgggag agacagcgcc aaccggaaag ttcggatcga gcgagtaggt
     3481 cgcgttctcc aacacattcg acatttcgcg gcgggctttg tcgagtgact ccagttcgtt
     3541 ttccgcacgc tgatagattc tcgccgaccg ggcacgattc agtagtttgt tcactctctg
     3601 gctgagctca aagtagtgtt tccaggtttg tactgacaga tactggtcga aaaactcctg
     3661 cgtctgcagc agatagagaa gatcgtcctg aacctgactt cccatgacga tgacatcgtc
     3721 agaaccgcga agatgggttg tcgcttcgac gagtcgtgac aggcggtacc aggtcattcc
     3781 catgaccagg gtcaccagca gaaacagtag aatcgacccg agaatgaccc gctgtgacaa
     3841 cccgaagcga cgcatgcttc ctcctgaagg ggctgattat accgcccgaa tgcggggttg
     3901 cgtcaagcgg aatcgcgagt ccggcatggc acgcagtcag aacgagtcga actcctcgcg
     3961 agtgcagctg tcatcctgcc agttgatgac ggcaaagccg gcaggaacgt aggatggata
     4021 gtaatctcca ccaccagcca ccagcgagtg gtcatactga ttccgccgca ccagaaaaaa
     4081 atcttcacgg gctgaaccgt cgagaaacac atctgaaggc tgcgtgcgca gatgcactct
     4141 gccccccagg tgccgcagca gcatgcgggt atccgaaccc acgaagggaa agcagatggc
     4201 ctctttgctg gtgatgttgc cgatctccgg cagtactttg tcgaggtgat ctccctggtc
     4261 ccagcgggcg ttcaactcgg ccgaatcgac aagcagggcg ttcaccgtca tttccggaaa
     4321 gcggaaaccc atcttcgggt gcgtcaccgc cagacgaaac agacgatcag ccacattcca
     4381 ggggcccagg ttcggattgg gttcatcgcg gtcttccttt tccgcctgat cgagcagatc
     4441 gttgatgaca ctgttccgtt cgcccttggt cagaaagccg gtcatgtagc aggccgcgcc
     4501 gattttcgcc agcaccttct cacggcagat cagactcttg attccaaaac gtgtcgctga
     4561 ttccgcggca ttcttgccgg gttcgagaaa ctcgcgcagt tctttcaacg ccgagtgcat
     4621 caccaacccg gcgagcgtca cgggaatgac ctgcggcggt tcctttggca aggcaaacag
     4681 cgaggcaatc cccttaaaat gaagtgccag catgggttgt cgcggatcag agttcgtact
     4741 gaaacggggt ggatagcaga tcgccgccag agcagccggg ttaagatcct tgcccccgag
     4801 gcgcatggca atctcatagt cgatcaagtt caccagttca ttgcgcagat actgaatcgg
     4861 gtgcgaatag tcaaaccaga tacggccttg gctcagaatc atgcacccat tcttctcgct
     4921 tccgttttcc ggctgactgg tatagtcctt gtaccccaat tcatagttct gtggaaaatc
     4981 cggatgctgg ttgaaatctc cggcgatgta gacatctttt tcacaaaaga tcgtgagagc
     5041 tttccacggc gggcaacccc atattcgcag cggaaccgtt gaatacaaaa gcatatcctc
     5101 ttttttcgac tcgtactccc aaccgaaaaa gagatcatag accgcttcct gcggcttcgc
     5161 gggatttcgc cattttgacg cactgaatcc ccggaaactg ctgccggggc cgagcatggt
     5221 cggtttcggt cgggtggctt ccttccagta attggggttg agaggcggca agcgctcact
     5281 cttggcaccc cgcatgccgt cgagcacgaa atgtccgatg accctgccgc cgcgctcttc
     5341 cggaaaagtc ttgaatttgt ccattttgga agaactgttt cccgagtcca gacggccttc
     5401 ctggccgtta cgaaatgaca atctcgtgga ctgacgaatc tcgagatacc cgggagtact
     5461 gatcttctcc atgtcaagca gatcactgcc ctgatcgatc atcccgcgac gggctatctt
     5521 gagcccttcg tgcccataga gtcttccacg gcgcagcacg ccgggagtca accggtacgg
     5581 tccataggca ccggtatcga gaatcccgcc atcatagagc agaaacgagg tcggcgtgaa
     5641 acgttcgagg atggctgtta ccttgcgatg actgtcatca acccgcccga ccgcctcaag
     5701 cagaaagtac cggtgcgctt cgtcaacggt cttggtgtcg agactgtttt tcgccgacag
     5761 taatcgcacc cgcacccgaa aatctccgat gattcgcgtc ttttcgagaa ccacacggcc
     5821 gccataggtc ccgttgccgt tgtgatcgag ttcgaggccg cgagtctcgc caaccacgaa
     5881 ggggcgatgt ttcgccggac tggtccaccc tttcaacgga atatagatac tgccgtgtgt
     5941 gacgaattgc gggttggccc gcatctcggc caacacacag ttcaaaccag catcagccgc
     6001 gcagagagcc agatagtcct tggcactctt cgacagcagg tgggcctgac cgcgctgaaa
     6061 gttcagaatc gcgcccacca tgatgccaag caccaggcag accatgagaa cgacgatgat
     6121 cgccgagcct cgcttctcac atcccggtaa cgttacatga atgctgatca tgggttcacc
     6181 caactcggac gaccacgaat ggcgaaacag gttctcaagg ccagagcatt ctcctgctcg
     6241 ctcaccgggc gattcggacg cacgaaggag acacgcacat gcacgagata cgggccatgc
     6301 ccatcgacct cataagg
//
LOCUS       DAORHD010000081         6315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12374_length_6315_cov_3.746006, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000081 DAORHD010000000
VERSION     DAORHD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12374_length_6315_cov_3.746006"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>6315
                     /locus_tag="PKO06_02895"
     CDS             21..>6315
                     /locus_tag="PKO06_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV68619.1"
                     /translation="MRGLISEYGLGERLKWGLQVGTPYLVTREVVESGAMSRNYRNWV
                     LNEDRTLVTGESIGLRARQVASPYVYQVLADEEAACAEGLSLPDRRIRFEKANLGNLA
                     RAVASREGDDGCFMAGESVALLQSIQTIEQLHTELLDLADDSDTREPGASRLLQSHAE
                     TSPLDAIDTVDTGTARGGGAAEPIAIIGMGCVFPGCPDPRRLFENLLARRHFIRPMPE
                     ERLDSGLFYREDRNQPLATYSRIGAWLDAVEFDAMQFRVPPRAAAVMDLGQKLALICA
                     RQALLDAGLLDSERFDRERTAVIIGNSMGGGASVENLRTVHVYEVTQRLKRFADETGI
                     GGLMQRLLERLNREIPTREITEDSLPGELSSLIAGRVAAVFDLHGPNFTVDAACGSGL
                     AAVAASVQALRQRTIDLALAGGVDTQMDPASFIKFSKVTALSGRGSFPFDHRADGFVM
                     GEGAGIVLLKRLSDAQRDGDRIYAVILGIGQSSDGKGRGITAPNPEGQRRAISRAWAD
                     AGVPFSRIGYLEAHGTGTRVGDEMELTSAAGLLETTSREGQPVLLGSIKANLGHTKAA
                     AGIAGLIKTAWVMQQRLVPPQPNFERFPESWNCRNLPFVVCREPALIPDKELFAGVSS
                     FGFGGTNHHVVLGEAPNTSRRADSFVIPTSFAPDQPEITASSSERTALMFPGQGSQYV
                     GMFSAWMSEPSFRDVLERAAKVFAHQNPHQNSLLSFISPDNLSQMTAEQIELAEGRLR
                     NTIIAQPAILAVSCGLLEVLRQRGVRADMTFGHSLGEYAALYAAGSLTLEQILDAVCV
                     RAQYMGSLPRNDLGAMGVVGAPADEVATELTAARAVPGYVTIANRNSPKQTVISGETA
                     VVETLLKRFADRGVLARRLPVSAAFHSDLVGGVAPLFRQRLETFPWKMPTMPVPANLT
                     GEWYPLPEERLSDDVSRTRMIDLLTRQVSGTVDFIDQVERAWKAGVRTFIEVGPKNVL
                     CRLVDEILGARAHRCLHTDHPRQNGHELVERVLAVSRDALRPAAVTPSRSLANAQAVD
                     ATDIASVPPGGSIEQTVRKAVEEISGYPASMIRPELDLEADLGIDTLKIFEIASRLRK
                     VFPALAGRRFDLSKIRTLQSIVELIRSSGPIEQPALPTNETPQVAVAHDPVDTSRAGT
                     NTGAPDHGGVARHVLGTGVCGTWQGIAALNVPTWCSRKTVLVRASGNDAFDEALEARL
                     RELDAWPILYAPDPSVQEALEQGSVPEIYRTAEVIFFPIAHERFGRRQRKSFYREQIL
                     HLLLLCRVLGKTLQGLVILTDLGGNLKTALIHESGAGFLSGVLPALCKGLVKDFPQLC
                     TRTIDLPSFTPEWVEAACRLGCTRGLPAMVGIDRDGRLTSQTVQAAPLLLHDAEAADQ
                     LRELLPRGSVVLATGGAAGITAHLLKRLARELAPRLIILGRSPDRSAFVQELRQTGAE
                     VWYLSCDLADGPAVDRTAEVIRRVYPRIDLLLHGAGLDISRHLSNKTPEEIDRVYRVK
                     VAGLVNLIDALGEENIGAVTSFSSVSSFFGNHGQVDYAAANGFLNAFIGRGRSRYLCL
                     AWSAWDRIGMVSRGPIYDILQANGVTFITPEAGEHFFLTELADFLQGTAKQRTVGFFG
                     EVGPGMSESGWLAPTIETRTNRLQISPDATGILWDHVIGGRCVIPGVVSLYQLVQAVL
                     SLQPEDWLVCEEVAYDFPIRRGHGETVRCLVQSEGEKQSLLVEDSNARGETLRRRHLQ
                     ALVRTETVTSSRRQEWLDRFERLELLLNETERGLVQGLKKQVVARSDLYSIFFHGPSF
                     QVLADVYRWSDRVLEAHPIPVPGLEDWRSLCGGRLWPFVLETAFHAAGLMCLMKVGAP
                     EYFLPHAIGRVSIVASAGQNLAGMRVLVEHLGTELEQRDALSLTVLRFQAVVVDGGRR
                     PVAVLEDLRMVAGRDRPADLRTFLATGFPFSVHETPLMILVTDDARRVASDQAALSHW
                     LHPEEQRIYAELLIDQRKADWLAGRMAAKILISDLIQRTLGMEVAPASFAVISAGAFP
                     QVKYLSVSAHALFQTWQVSISHTQGRAIAALAKGPLGVDIEQRRAITPEASERAFTAR
                     ERHDISPVDLITVWTAKEAVMKAT"
BASE COUNT         1167 a         1767 c         2016 g         1365 t
ORIGIN      
        1 gccggtacgc aacccgtttt ttgagagggc tgatttcgga atatggcctg ggcgaaaggc
       61 tcaagtgggg tctgcaggtc ggcacgcctt atctggtgac gcgagaggtg gtcgagagcg
      121 gagccatgag tcggaactat cgcaactggg tgttgaatga agaccgcacg ctcgtgaccg
      181 gcgagagtat cggtctgcgt gcccgacagg tggccagtcc gtatgtgtat caggttctcg
      241 cggacgaaga ggctgcctgt gccgagggcc tgtcgctgcc tgaccggcgc attcgcttcg
      301 aaaaagcgaa tctggggaac ctggctcgcg ccgtggcttc ccgggaagga gacgacgggt
      361 gcttcatggc cggcgagagt gtcgctcttc tgcaaagcat tcaaacaatc gaacagctgc
      421 atacagagct tctggatctc gctgatgatt cggacacgcg cgaacccggc gcgtcgcgtt
      481 tgttgcagtc gcacgccgag acgtcgcccc tggatgcgat tgatacggtg gacacaggca
      541 cggctcgcgg aggcggggct gccgagccca ttgcgatcat tggtatgggg tgcgtgtttc
      601 cagggtgccc tgacccccgt cggttgtttg agaacctcct ggccaggcgt catttcattc
      661 gacctatgcc cgaagagcgc cttgattcag gactgtttta tcgggaagac cgcaatcagc
      721 cgctggcgac ctatagccgt atcggtgcct ggctggatgc cgttgaattc gatgccatgc
      781 agtttcgtgt cccacctcgc gccgcggccg tcatggacct ggggcagaag ctggctctga
      841 tctgcgcccg ccaggcgttg ctggatgccg gactgctcga ttccgagcga tttgatcgcg
      901 aacggacggc tgtcatcatc ggcaacagca tggggggcgg ggcatcggtc gaaaacctgc
      961 gcaccgtaca cgtgtatgaa gtgactcagc gcttgaaacg ttttgccgac gaaaccggta
     1021 tcggtggctt gatgcagagg ttgctcgaac gtttgaaccg cgaaattccc acgcgagaga
     1081 tcaccgaaga ctcgctgccg ggtgaactca gcagtttgat tgccggccgg gttgcagcgg
     1141 tgttcgacct ccacggtccg aacttcaccg tcgatgccgc ctgtggatcg gggctcgccg
     1201 cggtggcggc tagtgtccag gctttgcgac aacgtaccat tgatctggcg ctcgcaggcg
     1261 gagtcgatac ccagatggat ccggcatcgt tcatcaagtt ctccaaggtc accgctcttt
     1321 ccgggcgtgg atcattcccg tttgaccatc gagccgacgg atttgtcatg ggtgaaggtg
     1381 ccggaattgt gctgttgaag cgactgagcg atgcgcaacg tgatggcgat cggatctacg
     1441 ccgtgattct tggtatcggc cagtcgagcg acggtaaggg ccgtgggatc accgctccta
     1501 accccgaggg acaacgtcgg gccatttctc gtgcctgggc ggatgccgga gtgccgttct
     1561 cgcggatcgg gtatctcgag gcgcacggga cgggaacccg cgtcggtgat gaaatggaac
     1621 tgacgtctgc cgccgggctg ctcgaaacaa cgtcacgaga agggcaaccg gtactcttgg
     1681 ggagcatcaa ggctaatctc ggccatacca aggccgctgc cggcattgcc ggactcatta
     1741 aaaccgcgtg ggtcatgcag cagcgtctgg tgccgccgca accgaacttc gagcgttttc
     1801 ccgaatcctg gaattgtcgg aatcttccct ttgtcgtctg tcgtgaacct gcgctgattc
     1861 ctgacaagga gctctttgcc ggagtctctt cgttcggctt tggtggaacc aatcatcatg
     1921 tggttctggg tgaagcgccg aacacgagtc gacgagccga ctcctttgtc attcccacga
     1981 gtttcgcacc agatcagcct gagataaccg cttcgagttc tgagcgtact gccctgatgt
     2041 ttccgggaca gggatcacag tatgtcggga tgttctcggc ctggatgtct gagccgtcgt
     2101 ttcgcgacgt tctggaacgg gcagctaagg tattcgccca ccagaaccct catcaaaaca
     2161 gcttgttgtc atttatctcc ccggacaatc tgtctcagat gaccgcggaa cagatcgagc
     2221 tggcagaggg acgtctgcgc aatacgatta tcgcccaacc ggccattctg gcggtcagtt
     2281 gtgggctgct cgaggttctg cgacagcgtg gcgtgcgggc ggacatgaca tttggacaca
     2341 gtctgggaga gtatgccgcc ttgtatgccg cgggttctct gacgctcgaa cagatcctcg
     2401 atgcggtgtg cgtgagggcg cagtatatgg gctcgctgcc gcgaaacgat ctgggagcca
     2461 tgggtgtcgt gggagcgccg gccgatgaag tggcaaccga actgacggct gcgcgtgcgg
     2521 tgccgggtta cgtgacgatt gcgaatcgca actcaccgaa acagacggtg atcagcggag
     2581 aaaccgcggt ggtcgaaacc ctgctgaagc gattcgccga ccggggtgtt ctggcgcgtc
     2641 gactgccggt gtcggccgcc tttcacagcg atctggtagg cggcgtggct ccactgttcc
     2701 ggcagcgact tgaaacgttt ccgtggaaga tgccgaccat gccggttccg gcaaatctga
     2761 ccggggaatg gtatccgtta ccggaggaac gtttgtctga cgatgtcagc cgcacccgta
     2821 tgatcgatct gctcaccaga caggtgagtg gtacggtcga tttcattgat caggttgaac
     2881 gagcctggaa ggcgggagtg cgcaccttca tcgaagtggg gccgaaaaac gtgctctgcc
     2941 gtctggtcga tgagattctg ggtgcgcgtg cgcaccgttg tctgcatacc gatcatccgc
     3001 ggcagaacgg acacgaactg gtcgagcggg tgctggcggt ttcgcgagat gctcttcgtc
     3061 ccgccgccgt cacgccttca cgatctcttg cgaatgcaca agccgtcgac gcgactgata
     3121 tcgcgagtgt gccaccaggc ggcagtatcg agcagaccgt gcgtaaggcg gttgaagaaa
     3181 tcagtggcta cccggcgtcg atgattcgac ctgaactcga tctcgaagcg gatcttggca
     3241 tcgatacgct caagatcttc gaaatcgcca gtcgcttgcg caaggtcttt ccggcactgg
     3301 ctggtcgccg ttttgatctg tcgaaaattc gtactctgca gagtattgtc gagctcatca
     3361 ggagttcggg gccgatcgaa caacctgcgc tacccacgaa cgaaaccccg caggtggccg
     3421 ttgcacacga cccggtagac acctcgcggg ccggcaccaa tacgggcgca cccgaccatg
     3481 gcggggtagc ccggcacgtg ttgggaacag gcgtctgcgg aacctggcaa ggcatcgctg
     3541 cgttgaacgt gccaacctgg tgttcgcgta agacggtact ggttcgcgcc agcggaaatg
     3601 acgcatttga tgaagctctt gaggcacgat tgcgtgaact cgatgcgtgg ccgattctct
     3661 atgccccgga cccatcggtg caggaagccc tggaacaggg atctgttccc gaaatctatc
     3721 gaaccgccga ggtgatcttt tttccgatcg cgcacgaacg gttcggccgc cgacagcgca
     3781 aatcgtttta tcgcgagcag attctgcatc tgctgctgct ctgccgggtg ttggggaaga
     3841 cgctgcaagg tctggtgatc ctgaccgatc tgggcgggaa cctgaagacc gcgctgatcc
     3901 acgagagtgg tgccggtttt ctttcgggtg ttttacctgc tctctgcaaa ggcttggtaa
     3961 aagactttcc gcaattatgc actcggacga tcgaccttcc atctttcacc ccggagtggg
     4021 tggaggctgc ttgccggctg ggttgcacgc gcggcttgcc ggccatggtg gggatcgacc
     4081 gtgacggcag actgacgagt cagaccgtgc aggctgcgcc gttgttgctt catgacgcgg
     4141 aagccgcaga tcaactgcga gaactgctgc cgcgcgggtc ggtcgttctg gccaccggcg
     4201 gcgctgccgg aatcaccgct cacttgctca aacgtttggc cagagagttg gcgcctcgtc
     4261 tgatcatcct cgggcgatcc cctgatcgat ctgccttcgt gcaggaactg cgccagaccg
     4321 gcgctgaagt gtggtatctc tcgtgcgatc tcgccgacgg accggctgtt gaccgcacgg
     4381 ccgaagtcat tcgtcgagtg tatccgcgaa ttgacctgct tctgcacgga gcgggtcttg
     4441 acatcagccg tcatctctca aacaagaccc cggaagagat cgatcgtgtc taccgggtga
     4501 aggtcgccgg cctggtcaat ctcattgacg cattgggaga agagaacatc ggtgccgtga
     4561 cgagtttctc gtcggtgtcg tcgttcttcg gcaaccatgg ccaggttgat tacgctgcgg
     4621 cgaacggctt tctgaatgcg ttcatcggtc gtggacgaag tcggtatctc tgtctggcct
     4681 ggtcggcctg ggaccgcatc ggcatggtgt cgcgcggccc gatctacgat atcttgcagg
     4741 caaatggtgt gactttcatc acgcccgaag ccggtgagca cttttttctg accgaactgg
     4801 ccgacttctt gcagggaacg gcgaagcagc gcacggtggg attcttcggc gaagtcggcc
     4861 ccggaatgag cgagagcggg tggttagcgc caaccatcga gacgcgcacg aacagactgc
     4921 agatctctcc tgacgccacc ggaatcctct gggatcacgt gatcggtggt cggtgcgtca
     4981 ttccgggcgt tgtcagtttg taccagttgg ttcaggccgt gctttctctg caaccggaag
     5041 attggttggt ctgtgaagag gtcgcgtatg actttccgat tcgccggggt cacggtgaaa
     5101 cggtgcggtg cctcgtgcaa tccgagggtg agaagcagtc cttgctcgtt gaagacagca
     5161 acgctcgtgg tgaaacgctg cgccgccgtc atctgcaggc tttggtgcga acggaaacgg
     5221 ttacgtcgag ccgcagacag gagtggctgg accgattcga acgactggag ttgttgctga
     5281 acgaaactga acgaggtctc gttcaagggc tgaagaaaca ggttgttgcg agatctgact
     5341 tgtactccat tttctttcac ggtccctcgt ttcaggtgct ggcggatgtc tatcgatggt
     5401 ctgatcgggt gctcgaagca catccgattc cggttcccgg tctcgaagac tggcgttccc
     5461 tgtgcggcgg ccgactctgg ccgttcgtgc tcgaaaccgc ttttcatgcg gctgggctga
     5521 tgtgtctcat gaaagtggga gcgcccgagt attttctgcc ccatgcgatc ggtcgggtct
     5581 cgatcgtggc aagcgccggg cagaatctcg ccggcatgcg cgttctcgtc gagcatctgg
     5641 gcacggagct cgaacagcgc gacgcattgt ccctgacggt attgcgtttt caggcggtcg
     5701 tggtcgatgg ggggcgtcgg ccagtggcgg tgcttgaaga cctgcggatg gtggctggtc
     5761 gtgatcggcc ggccgatctc agaacattcc tggccacggg gttccccttt tcggtgcacg
     5821 agacgcctct gatgatactg gttaccgatg atgcgcgccg ggttgcgagt gatcaggcgg
     5881 cgttgtccca ctggctgcac cccgaagaac agcgcatcta cgcggagctc ttgatcgacc
     5941 agcgtaaggc cgactggctc gccggtcgta tggcggcaaa gattctgatc agcgatctga
     6001 ttcagcgaac gctgggaatg gaggtcgcgc cggcatcgtt tgcggtgatc agcgccggag
     6061 cgtttccgca ggtgaagtat ctttctgtct cggcgcatgc cctgtttcaa acctggcagg
     6121 tgagcatcag tcatacccag gggcgagcca ttgccgctct ggccaaaggc cctctgggcg
     6181 tcgacatcga acaacgacga gccatcaccc ccgaagccag cgaacgcgcg ttcaccgccc
     6241 gcgagcggca cgacatctcg cccgtcgacc tgattaccgt ctggacagcc aaggaagcgg
     6301 tcatgaaagc cacgg
//
LOCUS       DAORHD010000082         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12657_length_6215_cov_3.304058, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000082 DAORHD010000000
VERSION     DAORHD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12657_length_6215_cov_3.304058"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="PKO06_02900"
     CDS             complement(<1..1337)
                     /locus_tag="PKO06_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNV68620.1"
                     /translation="MEHITITLKDGSRHSIERGQPISEVATKLPQDASLPILCARVNN
                     EIRSLDFKPEIDSTVEFLTYKSSDGQDAYRRSLTFVLARAVLELYRNARLVIGHSIGN
                     SFYYDLYTDVPVSNTILNYIRERMLKIIEKNEPFDKKVMTRTEAASVFKKEGYPEKAR
                     LVQNLNSDQVGVISSWKYLDLDHGPIVPSTGYLKVWDIKQYAHGFVLLFPDAKNPAEP
                     APISDQPKIFSVYRESKEWGKILEVNNVGRLNACIQSGEISDFIKVAEALHEKKIGQI
                     ADEITRRKDCRIVLIAGPSSSGKTTFSKRLCIQLRVNGLRPIALSLDNYFVNREHTPR
                     DEDGHYDFEDIHALDLHLFNEHLSELLKGREIQVPRFDFATGSRKKESDALRITEGQI
                     VIIEGIHGLNDELTESVEAKFKFKVYISALTQLVIDDYNRLSTTETRLIRRMVR"
     gene            1681..2772
                     /locus_tag="PKO06_02905"
     CDS             1681..2772
                     /locus_tag="PKO06_02905"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HNV68621.1"
                     /translation="MSSIRWKVPVLAPVIGLTLFWLAATLYHARPSTAERPGVGEAAA
                     VTVRVPLANGGLSERMSMKKVTKSDALWQRLLTPEQYRIARGKGTERAFCGSFYNHKQ
                     SGLYVCICCGLPLYHARAKFDSGTGWPSFFEPVAPENVSEHPDHSHGMVRTEILCTRC
                     DAHLGHVFDDGPPPSGRRHCLNSESLAFIPDDLTVDPEKPDRKLELATFGMGCFWGVE
                     ALFREVPGVVSAESGYSGGTVPWPSYEEVCEHGTGHAEVVLVRFDPAQVTYEQLLDVF
                     WKNHDPTQLNRQGPDVGDQYRSAVFSHAEAQKKAAEASKQKLEEAKAFARPIVTQITA
                     AAPFYRAEEYHQRYFEKHPEAGCHIIRRK"
     gene            2943..>6215
                     /locus_tag="PKO06_02910"
     CDS             2943..>6215
                     /locus_tag="PKO06_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV68622.1"
                     /translation="MRAFRTVLTWSLIAHLVGFAVFFVIGCGGGGGGGGSASPVSPAT
                     ETVWSTSLTQVQEALKTATGTWTAAETSVSLGFSETDAAALCGMVADPLLSSFTAAVK
                     ADVGVETVLAVAPNGTSLPRRFTWREKDGKDWTSPVKNQGRYGTCVAFAAIAALEMSL
                     KIAQQQSGVSYDFSEWHLWHLGTKGKSPAPGGWFPSGAVECLKTRGAVSEDLCPYTAI
                     PSTPTPPSDARWFPISNWSVVSGRSALKQALLEGPVMAAMRVRSDFCFYRGGIYSPVA
                     GRELGDHAILLIGYDDDQGCWIAQNSWSASWGENGFFRIKYGQINDQGLRVAVTKGTA
                     AGQTQTSSGTDEAMLGFPNPVVQLGPYVREVTATSAVICWATDQSTTAQVEYGAGPTF
                     SRVAQKEAPAATHSMPLLGLQESTAYQFQVTSTTPTGQTIRSNVQSFITLSVGSAPVV
                     APAIFNVNVTGITKTSAMVSWSTDQAATGKIYYVTGTIASTASDLRLRTAHSITLNNL
                     QGGTTYSFYVEASASSLVAVSDTSLFTTLDSTPPAISEVSVPTPTLSGTKASVVWLTD
                     EAATSQVEYGFSPAYGQLTNLVPTLTFGHQVNLTNLLPQTTYHYRVRSKDANGNEGTS
                     SNGTFTTPDSVPPVLSTIRTSPITGTTATINFTTNETATVQVEYGPGAFTAITAPETN
                     TTFSILLSGLTPKVVYKFRVRAKDAANNEAVTADLQFTTVDSAAPVISNISVAPETLT
                     GTTAQVTWLTDEAATTQVEYGLTAAYGLSTTIDTTKVTGHLAKLIALLPNTTYNYRVR
                     SKDVSGNETVSGNNTFTTLDSQPPTISNIRVLPITGTTGNVTFTTSKPATIVIEYGVG
                     IFSALASTTTLATTHEILLSGLLPKSAYRYRIYATDSAGNVGISAILSFTTLDTVPPV
                     ITGIIVSSITNTSATVAWTTDKAATSQVIWGSSTTYDKGPIVDSTLLKNHSIKINLDD
                     SSVPIWNFQVSSTDAAGNVATSTNQTFQVPLYLYDVNVTAIGTDSATIVWNSNATASS
                     LVEYGLSSLYGNQAYTLPFPNTGTRNHSVTVSGLVPFATYYFRVRSKEPTGREAVSQG
                     FSF"
BASE COUNT         1259 a         1751 c         1893 g         1312 t
ORIGIN      
        1 cgcaccatgc ggcggatcag gcgggtttcg gtggtcgaaa ggcggttgta gtcgtcgatg
       61 accagctgcg tcagcgcaga aatgtagacc ttgaacttga acttggcttc gaccgattcg
      121 gtgagttcgt cattcaggcc gtggatgcct tcgatgatga cgatctgacc ttcggtgatg
      181 cgcagggcgt cgctctcttt cttgcgcgac ccggtggcga agtcgaagcg tggcacctga
      241 atctcgcggc ctttcagcag ctcgctcaga tgttcgttga acagatgcag atcgagggcg
      301 tgaatgtctt cgaagtcgta gtgcccgtct tcatcgcgcg gggtgtgttc gcgattgacg
      361 aaatagttgt cgagggaaag cgcgatcggg cgcaggccat tgacccgcag ctgaatgcac
      421 aggcgcttgc tgaacgtggt tttgcccgaa ctcgatggcc cggcaatgag tacgatgcgg
      481 caatctttgc ggcgcgtgat ctcgtcagcg atctgaccga tcttcttctc gtgcagggct
      541 tcggcgactt tgatgaaatc agaaatttca ccgctctgga tgcaggcgtt gagacggccg
      601 acgttgttga cctcgagaat cttgccccat tccttgcttt cgcgatagac cgagaagatc
      661 ttcggctgat cactgattgg tgctggttcg gccgggttct tcgcgtcggg aaagagcagc
      721 acgaaaccgt gggcatactg cttgatatcc cagaccttga gataaccggt ggaaggcaca
      781 atcgggccgt gatcgagatc caggtacttc cagctgctga tgacgccgac ctgatcggag
      841 ttcagattct gcaccagacg tgccttctcg ggataccctt ccttcttgaa gacggacgcg
      901 gcttcggtgc gcgtcatcac cttcttgtcg aacggttcgt tcttctcgat gatcttgagc
      961 atgcgctcgc ggatgtaatt gagaatcgta ttcgatacgg gcacgtccgt gtacaagtcg
     1021 taatagaagc tgttgccgat cgagtgccca atgacgagtc gggcgtttcg atacagttcg
     1081 agcacagcac gggccagcac gaatgtgaga ctgcgacggt aagcgtcttg tccgtcagag
     1141 gatttgtagg tcagaaactc gacggtgctg tcaatctcgg gtttgaagtc gagtgagcga
     1201 atttcgttgt tgacgcgggc acagaggatg ggcagtgaag catcctgcgg gagcttggtc
     1261 gcgacttcac tgatgggctg gccgcgttcg atggaatggc gcgagccgtc tttaagcgtg
     1321 atcgtgatgt gttccatgtc gtgttcttcc ttcagagatc gaaataaaaa cccccgagcc
     1381 tgttgcggcc cgggggtcgg actcagcaga ctaccgaaga actgccccgc tccgcatact
     1441 caggccccgc gctcgttgac gagccacggt cgccgccgtc ggtggggaaa acgggtgcac
     1501 aattcctccc tttcatcctt gattagcggc tagcgtacca tcctcttttc gctgtgtcaa
     1561 ttcgaaatcg gtttgcgcgg ggagagtttt cgatgcacgg agtttgtaat tgacaggata
     1621 acggtatcgg aataacttga gaaggagagt catacggaaa tgaaaggagg tcatgctatc
     1681 atgagctcca tcagatggaa ggttccggtg ctggcgccgg tgatcggtct gaccctgttc
     1741 tggctggcgg ccacactgta tcatgctcgt cccagtacgg ccgaacgacc aggcgtcggc
     1801 gaggctgctg ccgtcacggt gcgcgtgccg ttggccaacg gaggtctgag cgaaaggatg
     1861 tctatgaaaa aagtcaccaa gtctgatgcg ctctggcagc gcctgctcac ccccgagcag
     1921 tatcgcattg cccgcgggaa aggcacggag cgagcattct gtggatcgtt ctacaaccat
     1981 aagcagagtg gtctgtatgt ctgcatctgt tgcggtttgc cgctctatca cgctcgggcg
     2041 aaattcgact caggcacggg ctggcccagt ttcttcgagc cggtcgcgcc tgagaacgtc
     2101 agcgagcacc ccgatcacag ccatggcatg gtgcgcaccg agattctctg tacacgatgc
     2161 gatgctcatc tggggcatgt cttcgacgac ggcccaccgc cgtccggtcg gcggcattgt
     2221 ctgaattccg agtcgctggc gttcattccg gacgatctga cggtggaccc cgagaaacct
     2281 gatcgaaagc tggaactggc gacgtttggc atgggatgtt tctggggcgt cgaagccctg
     2341 tttcgcgagg tgcccggtgt cgtatcggcc gagagcggtt attctggcgg cacggtgccg
     2401 tggcccagtt acgaagaagt ctgcgaacat ggcacaggtc acgcagaagt tgtgctggtg
     2461 cggttcgatc cggcgcaggt gacgtatgaa cagctgctcg acgtcttctg gaaaaaccat
     2521 gacccgactc agctgaatcg gcagggcccg gatgtcggcg atcagtatcg ttcggcggtt
     2581 ttctctcatg cggaagccca gaaaaaggcc gctgaggcgt cgaagcagaa gcttgaagaa
     2641 gccaaggcgt tcgcccggcc gatcgtgacc cagattaccg ctgccgcccc cttctaccga
     2701 gctgaagaat atcaccagcg ctacttcgag aagcacccgg aagccggctg ccacatcatt
     2761 cgccgcaagt aaccccgcct cagggcaaac ccccgtggca gcctcgagcg acgggtgcct
     2821 gagtatccgt ttaccctgcc gccataactg ccccctattc cggcgcgggg aaaatgccga
     2881 aagagagatt gccacatctc tgtttaggcg cggattttct ctatgcggcg ggggtaagcc
     2941 gaatgcgagc atttcggaca gttctgacct ggtctctcat cgcccatctg gtgggctttg
     3001 ccgtgttttt tgtcatcggt tgcggcggtg gtgggggagg cggtggatct gcgtcgcccg
     3061 tgtcacccgc gacggaaact gtctggtcga cttcgctcac gcaggttcag gaagccctga
     3121 agacggcgac cggaacctgg actgccgcgg aaaccagtgt cagccttggg ttcagcgaga
     3181 ctgacgccgc tgcgctctgc ggcatggtgg ctgacccgct gctctcatcc tttacggcgg
     3241 cggtcaaggc cgatgtcggg gttgaaaccg tgctcgcggt tgctccaaac ggcacctcgc
     3301 tgccgcgacg ctttacgtgg cgcgagaaag atggaaaaga ctggacgagt ccggtcaaga
     3361 accagggtcg gtatggcacc tgcgtcgcct ttgcggccat tgccgcactc gaaatgtcgt
     3421 tgaagatcgc gcagcagcag agtggtgtga gctacgactt ctcggaatgg catctgtggc
     3481 atctgggcac caagggcaag tctcctgcgc caggtggatg gtttccgtct ggtgccgtcg
     3541 aatgtctgaa gacacggggg gcggtcagcg aagatctgtg tccgtataca gcgattccat
     3601 cgacgccaac tcctccgtct gatgcgcggt ggttcccgat cagcaattgg tcggtggtga
     3661 gcggtcgctc ggccttgaag caggctctgc tggaaggccc ggtgatggcc gccatgcgag
     3721 tgcgttccga tttctgtttt tatcgcggcg gcatctacag cccggtcgcg ggtcgagaac
     3781 tcggtgatca tgccattcta ctgattggct acgacgacga tcagggctgc tggattgcgc
     3841 agaattcctg gtcggcttcg tggggtgaaa acgggttttt ccggatcaag tatggccaga
     3901 tcaacgatca ggggctgcgc gtcgctgtca cgaaggggac ggcggccgga cagacgcaga
     3961 ccagctcggg aaccgacgaa gcgatgctcg ggtttcccaa cccggtggtg cagttggggc
     4021 cctatgtgcg tgaggtcacg gcaacgtcgg cggtgatctg ctgggcgacc gatcagtcga
     4081 ccacggcgca ggtcgagtac ggtgccggcc cgaccttctc tcgtgtcgca cagaaagaag
     4141 ctccagcagc gacgcattcg atgccactgc tgggactgca ggaaagcacg gcctatcagt
     4201 ttcaggtgac ctcgacgaca ccgaccggtc agacgattcg gtcaaatgtc cagagtttca
     4261 ttacgctgtc tgttggcagc gcgcccgtgg tcgccccggc cattttcaac gtgaatgtca
     4321 ccggcatcac caagaccagt gccatggtga gctggagcac agaccaggcg gcgaccggca
     4381 aaatctatta tgtgaccgga acgattgctt cgacggcatc tgatctgcgt ctacgcactg
     4441 cgcattcgat taccctcaac aacctgcagg gagggacgac ctactcgttc tacgttgaag
     4501 catcggcgag cagtctggtg gcggtttccg atacctcgtt gtttacgacg ctcgacagta
     4561 cgccgccggc gatcagcgaa gtgtcggtgc cgacgccgac gctgtcgggc acgaaggcga
     4621 gcgtggtctg gctgacggat gaagcggcga cttcgcaggt cgagtatgga ttctcccctg
     4681 cttacggaca gttgaccaac cttgtaccca cactgacgtt cggacatcag gtgaacctga
     4741 ccaacctgct gccacaaact acgtatcatt atcgtgtgcg atcaaaggat gcgaacggca
     4801 atgagggaac gagcagtaac ggcaccttca cgacacccga ttcggtgcca ccggtacttt
     4861 cgaccattcg taccagtccg attacgggga ccacggcaac gatcaacttc accacgaatg
     4921 aaaccgcgac cgttcaggtc gaatacggac caggcgcgtt cacggccatt accgcacccg
     4981 agaccaatac gacattctcg atcctcttga gcggtttgac gcccaaagtg gtgtacaagt
     5041 tccgggtgcg cgcgaaggat gccgccaaca acgaagcggt gacggccgat ctgcagttca
     5101 cgacggtcga ctcggctgca ccggtaatct cgaatatcag cgtcgcacca gagacactga
     5161 ctggtacaac cgctcaggtc acctggctga ccgatgaggc ggcaacaact caggtcgaat
     5221 acggactgac cgctgcctat ggcctctcga ccacgatcga tacgaccaaa gtcacggggc
     5281 atctggcaaa gctgatcgcg ctgctgccga acaccacgta caattatcgc gtcagatcga
     5341 aagatgtcag cggcaacgaa acggtctcgg ggaacaacac cttcacgaca ctcgattcgc
     5401 aaccgccgac gatttcgaac atccgcgtgc tgccgatcac cggcacgaca gggaacgtca
     5461 cgttcacgac gagcaagccg gcgacgatcg tcatcgagta cggcgtcggc attttttcag
     5521 cgctggcttc gacgacgaca ctggcaacga cccacgagat tttgctttcg gggctgctgc
     5581 cgaagagtgc ctaccggtac cgcatctatg ccactgactc ggccggaaac gtgggcatct
     5641 cggccattct ctcgttcacg acgcttgaca ccgtgccgcc ggtgatcacc ggcatcattg
     5701 tctcgagtat caccaatacg tcggcgacgg tggcctggac gaccgacaaa gcggcgacct
     5761 cgcaggtgat ctggggttcg agcacgacgt atgacaaggg accgatcgtt gactcgacgc
     5821 tgttgaagaa ccattcgatc aagatcaacc tggacgatag cagtgtaccg atctggaact
     5881 ttcaggtctc gtcgaccgac gccgccggga atgtggccac gtcgaccaac cagacatttc
     5941 aggtgcctct gtacctgtac gatgtgaacg tgaccgcgat tggaacggat tctgcgacga
     6001 ttgtctggaa cagcaacgcc acggccagct cgctggtcga gtatgggctg agttcgctct
     6061 acggcaatca ggcctatact ctgccgttcc cgaacacggg cacgcgcaac cactcggtga
     6121 cggtgagtgg tctggtgcct tttgccacct attactttcg ggtgcgatca aaagagccga
     6181 cagggcgcga ggcggtctcg caggggttca gcttc
//
LOCUS       DAORHD010000083         6205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12680_length_6205_cov_3.721951, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000083 DAORHD010000000
VERSION     DAORHD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12680_length_6205_cov_3.721951"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKO06_02915"
     CDS             complement(<1..784)
                     /locus_tag="PKO06_02915"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HNV68623.1"
                     /translation="MRGRFNLKMVSVLLALILWFYVNFVISPLQKRTVTVPVDYRNIP
                     VNMLVTPTKTTVEVSLTASRREFILAGADKVQAYVDLLSLRTTPTYFPIRTIANPGIS
                     VEFTRPAQIAFAGQILKIQEFPVVVELKGAPAEGFMAEQAVVAPAKVKVEAPAEVLER
                     IVACKVQISLGDVKNSVSERRKVFAMLANGEPTAQATISPESVSVDVPVKAGYPTREL
                     PVKPQFLNKLPEGLRLEGFTVNPATMTISAPLRVLDDLKELLT"
     gene            complement(781..1596)
                     /gene="cdaA"
                     /locus_tag="PKO06_02920"
     CDS             complement(781..1596)
                     /gene="cdaA"
                     /locus_tag="PKO06_02920"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNV68624.1"
                     /translation="MFHSALQILWSVMRDVSVMDLLDVALVTWAIYRVLLLIEGTRAF
                     NLLKGLGILVLFLAFTRDLDFHTVNWVLSNTLPAGFLALVVIFQPELRRALEDIGRGK
                     FLVDDFAGGENLEEVIDAVARTIDTCAKKRIGALILIEQEIGLKDFIEKGVPLHSYIT
                     AELLNTIFLPFTPLHDGAVIIKGPYIEAASCFLPITTNPDLARELGTRHRAAVGITEI
                     TDALALIVSEETGTISMARSGKITRELTDDKVRELLSGAFRRVHKPRRLLSLR"
     gene            complement(1600..2499)
                     /gene="dapA"
                     /locus_tag="PKO06_02925"
     CDS             complement(1600..2499)
                     /gene="dapA"
                     /locus_tag="PKO06_02925"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003317384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNV68625.1"
                     /translation="MKKKQPILLQGSWVALVTPFNRGRIDRPALKKLLDFHLQNDTDG
                     LVISGTTGESATLSAEEKRELMSECTTHLQGRIPLMLGTGGNCTATATENTAHAADWG
                     AQAVLVVTPYYNKPTQPGLIAHFTKVATATTLPVVLYNVPGRTGVNLLPSSVLALANV
                     PNIRAVKEASGNLEQAMEILRFAPRDFVLFSGEDALNLPLMAVGARGTISVTANVAPR
                     AMKEFNDAALAKNWDKAREIHFSLLDLHRHLFLESNPIPVKTALAHMGLIKEEFRLPL
                     VKAADATREKLVSLLQRLKLKGR"
     gene            2645..3346
                     /locus_tag="PKO06_02930"
     CDS             2645..3346
                     /locus_tag="PKO06_02930"
                     /inference="COORDINATES: protein motif:HMM:NF014494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNV68626.1"
                     /translation="MSHTNALNEIPGLCGLWVVCRPESPEVLADPVPAIAGVSIRWEY
                     AVSRSAAEAGRCPEIGRRGRFFGTHDQETICAKMELYDLVVVQPLSLNSLAKIALGIR
                     DSLPAAVVLTALAAGKPLFLAQEGMDAATAGMHPHLTKLYRRYWQDLTGGPVIGFTRA
                     GLPEKLEVWFRRRRDALRLSAERRAGRPVITRDDVREAWQAMVPLVIPRGALLTDLAR
                     EEAGALGVTLRIEEP"
     gene            3366..4007
                     /locus_tag="PKO06_02935"
     CDS             3366..4007
                     /locus_tag="PKO06_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNV68627.1"
                     /translation="MEKMLSLSLRDFSQAVASDSFSPGGGGCSAYAGALGASLGHMVL
                     TLTIGKKKYAEFDEANRRLQAEMKQLAEALLGCVERDHKGCERILEAMALPKDTESEK
                     MRRKSAIAEASKVANEAPIDVTRLALKVLQLMKNGIAHVNRNCITDWACGALQAYAAL
                     EGAAMNAKINCGSVEDHKYCSDVRDEMRNTLGEGRRLLDEIRNHVHSSIDASN"
     gene            4429..5052
                     /locus_tag="PKO06_02940"
     CDS             4429..5052
                     /locus_tag="PKO06_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68628.1"
                     /translation="MSIIELSDLYVLLNERRVHNLNFVTGTHFAPHLFDALLRAREQG
                     VELPVVWNTSGYESVDLLQVLEGLVDVYLPDIRYADNAVAAQLSKVGNYVEVDRAALL
                     EMYRQVGPLVLDAEEIAVRGMIVRHLVLPGGLAGTRPSLTWLRDHIGTDVSVSLMCQY
                     FPAHQAHQLPGLDRRLTDDEYRQAIDVLEELGFEKAWAQDPYEPGCA"
     gene            5161..5925
                     /locus_tag="PKO06_02945"
     CDS             5161..5925
                     /locus_tag="PKO06_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV68629.1"
                     /translation="MSGHNKWAKVKHIKGKMDAIRGRMFTKLIKEITLCARNGGGDPN
                     SNPKLRAAIQAAKDQNMPKDNVERAIKKGTGELEGVTYEEVTYEGYAPGGVAVIVRCL
                     TDNKNRAVSEIDKILSKGGGNRGAPGCVSFMFDNKGYFFLSKEDNPKLTEEALTDIVL
                     DAGADDLKALEDGFEVNCEPSAFLDVRPKLEAKGLKITEAKVAMFPKTTVAVKGADVA
                     KVLRLIENLEEHDDVNDVYSNHEISDADMEQYMAAS"
BASE COUNT         1302 a         1723 c         1895 g         1285 t
ORIGIN      
        1 cggtcagcag ttccttgaga tcgtcgagaa cgcgcaacgg cgcgctgatc gtcatggtcg
       61 caggattgac cgtgaagcct tccagacgga ggccctcggg caacttgttc agaaactggg
      121 gtttcaccgg cagttcacga gtcggatatc cggccttcac cggaacatcg accgagactg
      181 attcggggga gatggtagct tgcgccgtgg gctcaccgtt cgccagcatg gcgaacacct
      241 tgcgtcgttc gctgaccgag ttcttgacgt cgccaagcga gatctgaact ttacaggcga
      301 cgatgcgttc caggacttct gcgggggcct cgaccttgac cttggcgggg gcgaccactg
      361 cctgctccgc catgaatcct tcggccgggg cgcctttcag ttccaccacg accgggaact
      421 cctggatctt cagaatctga ccggcgaagg cgatctgggc gggacgcgta aactccacgc
      481 tgatgccggg gttcgcgatc gtgcgaatcg gaaaatacgt cggggtggtg cgcaggctga
      541 gcaggtcgac ataggcctgc accttatccg cgccggcaag aatgaactcg cgtcgcgagg
      601 cggtcagcga aacttcgact gtcgtcttgg tcggtgttac cagcatgttt accggaatat
      661 tgcgatagtc gactggtacg gtaacggttc gtttctgaag aggcgagatg acgaaattga
      721 cgtagaacca gagaatcagt gccagcagca ccgagaccat cttgagattg aaacggcctc
      781 tcatcgcagt gacaacaacc tcctgggttt gtgaacccgg cggaaggctc cggagaggag
      841 ctcgcgcacc ttgtcatccg tcagttcgcg ggtgatcttg ccgctgcggg ccatcgaaat
      901 cgtgccggtt tcttcgctga cgatcaatgc cagcgcatcg gtgatctcgg taatgccgac
      961 cgcggcgcga tgccgggttc cgagctcgcg ggccaggtcg gggttggtcg tgattggcag
     1021 aaagcagctg gcggcctcaa tataggggcc cttgatgatg acggcgccgt cgtgcagcgg
     1081 cgtgaatggc agaaagatcg tgttcagcag ctcggccgta atgtaactgt gcaacggcac
     1141 gcctttttcg atgaagtcct tgagtccgat ttcctgctcg atgagaatca gggccccaat
     1201 gcgtttcttg gcacaggtgt cgatggtacg agccacggca tcgatgactt cttcgaggtt
     1261 ctcgccgccc gcgaaatcat cgacgaggaa cttgccacgg ccgatgtctt caagtgcgcg
     1321 gcgcagctcg ggttggaaaa tgaccaccag ggcgagaaag ccggcaggaa gcgtgttcga
     1381 cagaacccag ttcacggtat gaaaatcgag gtcgcgcgtg aacgccagaa aaagtaccag
     1441 tatgccgagg cccttcagca gattgaacgc gcgcgtgcct tcgatgagaa gcaaaacacg
     1501 atagatcgcc caggtcacca gcgccacgtc cagtagatcc atgaccgaga catctcgcat
     1561 cacggaccag aggatctgca gcgcggaatg gaacatggat cagcgcccct tcagcttcag
     1621 acgttgcagc aacgagacaa gcttttcgcg ggtggcatcg gctgctttga ccaacggcag
     1681 gcgaaactct tccttgatca gacccatgtg agccaatgcc gttttcacgg gaatggggtt
     1741 gctttcgagg aagagatggc gatgcaagtc gagcagcgag aagtggatct cgcgtgcctt
     1801 gtcccagttt ttggccagag ccgcatcgtt aaactctttc atggctcggg gagccacatt
     1861 ggcggttacc gagatggtac cgcgcgcccc gaccgccatc aggggcaggt tcagcgcgtc
     1921 ttcgccggag aagagcacga aatcgcgggg ggcgaaccgc agaatctcca tggcttgttc
     1981 gagattgccg cttgcctctt tgaccgcgcg aatattcgga acattcgcca gagccaggac
     2041 gctcgaaggc aggagattaa ccccggtgcg accggggacg ttgtagagca ccaccggcag
     2101 cgtggtcgcg gtcgcgactt tggtgaaatg agcgatcaga ccaggctgtg ttggtttatt
     2161 gtaatacggg gtcacgacca gaacggcctg agcaccccaa tccgcggcat gcgccgtgtt
     2221 ctcggttgca gtcgcggtgc agttgccacc tgtgccgagc atgagaggaa tgcgcccctg
     2281 gagatgtgtc gtgcactcac tcatcagttc ccgcttttct tcggcggaca gcgtggccga
     2341 ctcgcccgtc gtgccggaga ttaccagccc atcggtgtcg ttctgcagat gaaagtcgag
     2401 cagtttcttc agggcgggtc gatcgattcg acctcgattg aacggagtca ccagggctac
     2461 ccacgagcct tgcaggagga tcggttgctt ctttttcatg atgcgttcgc gtcacctcag
     2521 gagagtgatg cacctaccat aatatgaaac tcccttttcc gccaaacatt ttatgccgaa
     2581 aaatgtactg cgacgtcttc cggttgactt catgagggaa ggcggcgtac cctgccattc
     2641 agacatgtca cacacgaatg ccttgaacga gattcccggc ctgtgcggtc tctgggttgt
     2701 ctgccgcccg gaatcgcccg aggttctcgc tgatccggtt cccgccatcg caggggtatc
     2761 gatacggtgg gaatacgcgg tctcgcgctc agccgccgaa gcggggcgat gccctgagat
     2821 cggccgtcgc ggtcgttttt tcggaaccca cgaccaggaa acgatctgcg cgaagatgga
     2881 gttgtacgat ctggttgtgg ttcagccgct gagtttgaac tcgctggcga agatcgctct
     2941 cggtatcaga gactctctgc ccgcggcggt cgtcctgaca gctctggctg ccggcaagcc
     3001 cctgtttctc gcgcaggagg gaatggacgc ggcgacggcc ggaatgcacc cgcatctgac
     3061 caagctctat cgccgctact ggcaagatct gacgggaggc ccggtgatcg gattcactcg
     3121 cgcagggctg ccggagaagc tcgaggtctg gttccgccgt cgccgtgacg ctttgcggct
     3181 gagcgctgaa cggcgagccg gacgaccggt gatcactcgt gacgatgtcc gcgaagcctg
     3241 gcaggccatg gttccgctgg tgatcccgcg cggggctctg ctgaccgatc tggcacgcga
     3301 agaagccgga gcgctgggtg tgacgctgcg cattgaagaa ccctaatccc gaattgaggc
     3361 taaccatgga aaaaatgctt tctctctcgt tgcgtgattt cagtcaggca gtggcttccg
     3421 attcattttc tccgggcggt ggcggctgtt ccgcctacgc cggcgctctt ggtgcctctc
     3481 tcgggcacat ggtgctcacc ctcaccatcg gtaagaaaaa gtacgcggag tttgatgaag
     3541 cgaatcgcag gctgcaggca gagatgaagc aactcgccga agctctgctc gggtgcgtgg
     3601 aacgagatca caaaggctgt gagcggattc tcgaggccat ggcgctgccc aaggacactg
     3661 agtccgagaa gatgcgccga aaatctgcga ttgctgaagc cagtaaagtc gcgaacgagg
     3721 ctccgattga cgtgacacga ctggcactca aggtgctgca gttgatgaag aacggcattg
     3781 cccatgtcaa tcgcaactgc atcacggact gggcgtgcgg ggctctgcag gcgtatgccg
     3841 cacttgaggg cgccgccatg aacgcgaaaa tcaactgcgg ttcggtcgaa gatcacaaat
     3901 actgttccga tgttcgtgac gagatgcgaa acaccctcgg agaggggcgc cgcctgcttg
     3961 acgaaattcg caatcacgta cactcgtcga tcgacgcatc gaactgatct cgtcgatcgt
     4021 ccgagccgaa gtcggccata accctttaca gccaggaatt cgcgcctgta tggtaccgaa
     4081 ctctcggttt cgccacggtt cgctttctct cgctgccttt cgacaaagga tcgcctgatg
     4141 tcgcgtctga ctctggatga aagccgccgc cgcgtccaac ggctgtggga gatgttcgat
     4201 ccgtgtgttc tctgcccccg ccgctgcggc gcacggagac gttcagggca gattggcgtc
     4261 tgcggcgccg gccccgatat ccgggtagca gcctggcacg cgcatcgggg cgaagaaccc
     4321 ccgctttcgg gtacccgcgg ctcggggacg attttctcgt ctcactgccc gatgaagtgt
     4381 ctgttttgtc agaattttcc attctcgcaa ctcgggaatg gaaagacgat gagtattatc
     4441 gagttgtctg acttgtacgt tctgctgaat gagcgacggg tgcataatct caacttcgtc
     4501 acgggcacgc attttgcccc gcacctcttt gacgcattgc tgcgggcacg ggagcaaggc
     4561 gtcgaactgc cggttgtctg gaatacgagc ggctacgagt cggtcgatct gctgcaggta
     4621 ctggaagggc tcgtcgatgt gtatctgccc gacattcgct atgcggacaa tgccgtggcg
     4681 gcccagttgt ccaaggtcgg caactatgtc gaggtcgacc gtgccgcttt gctcgaaatg
     4741 taccggcagg ttggcccgct ggtgctcgat gcggaagaga ttgccgtcag aggcatgatc
     4801 gtcagacacc tggtactgcc gggaggtctg gccggaacgc gcccgtcgct gacctggctg
     4861 cgtgatcaca tcggaaccga cgtttctgtc agcctgatgt gccagtattt tcctgcccat
     4921 caggcacacc aactgcctgg cctcgaccga cgtctgacgg acgacgagta tcgacaggcg
     4981 atcgatgttc tcgaagaact cggcttcgag aaagcctggg cgcaggatcc ctatgagccg
     5041 ggctgcgcgt gagagatggc cctgttgatc agcgaaaaag attgattccc gggcgaatta
     5101 tgatatactc agtccgcatc tgtaacgtca aggctgcaca gcaggcttca ggaggaaatg
     5161 atgtcaggtc ataataaatg ggcaaaggtt aagcacatta aggggaaaat ggatgcgatc
     5221 cgcggtcgca tgttcacgaa gctgatcaaa gaaatcacgc tttgtgcccg taacggcggt
     5281 ggagatccga acagcaaccc caagcttcgc gccgccattc aggccgcgaa agaccagaac
     5341 atgcccaagg acaacgtcga acgtgccatc aaaaagggaa cgggtgaact cgaaggggtg
     5401 acctacgaag aagtcaccta tgagggatac gctcctggcg gcgttgcggt gatcgttcgt
     5461 tgcttgaccg acaacaaaaa ccgcgctgtt tctgaaatcg ataaaatcct cagcaagggt
     5521 ggcggcaatc gcggggcccc cggctgcgtg tcattcatgt tcgacaacaa agggtatttc
     5581 tttctgagca aggaagacaa cccgaagctg accgaagaag cattgaccga catcgtgctc
     5641 gatgctggcg cggacgacct caaggcactc gaagacgggt ttgaagtgaa ctgcgaaccg
     5701 tcggcttttc tcgatgtgcg ccccaaactc gaagccaagg gtctgaagat caccgaagcc
     5761 aaggtcgcca tgtttcccaa gaccacggtc gcggtgaagg gcgcggatgt cgcgaaggtt
     5821 ctgagactca tcgagaatct cgaagagcat gatgatgtga atgacgtcta ttccaaccac
     5881 gaaatctccg atgccgacat ggagcagtac atggcggcct cctgacgagg catctccgtg
     5941 acatgaaatc ccggaagccg cgcctcgact gaggttcggt ttccgggttt ttcattcccg
     6001 acgtgacacc tgattttacg ggagaggaac aatgattgat tctctgcagg gcattctgac
     6061 agtaaaacaa ccgcatcggt gcgtgatcga gaatgatgga atgggcttcg agatgctgat
     6121 tcccttcagc accttcaagc agttgcgcga ggtcggcagc ggtgctcgtc tgctgtgcca
     6181 tctggtctgg cgtgaggacg gaccg
//
LOCUS       DAORHD010000084         6190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12715_length_6190_cov_3.468297, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000084 DAORHD010000000
VERSION     DAORHD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12715_length_6190_cov_3.468297"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(290..1345)
                     /locus_tag="PKO06_02950"
     CDS             complement(290..1345)
                     /locus_tag="PKO06_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNV68630.1"
                     /translation="MMFVKRFRVFMLGVLLVCGVVPGFAAGGRVALLIGNAAYNNVPP
                     LENPPNDVRAFAEALRQVGFDVVVKQDTDLRTMDEAITDFWNRLKGQEAGLFYYSGHG
                     IQLNGENFLVPTNAVIEKEMDIKHGCFPAQKLLDFMDDAKAKTNIVILDACRNNPFKR
                     SARSAAMGLARMDAAGQMLIAYATDPDKTAADGEPGGNSPYMKHILKNMMNPGMPVER
                     MFKLVRVGVLDETGQKQRPWELSCLTQDFAFVPGGSSGYEAVSGPSVKIEPPTVSAST
                     GTNESSAPAGWRTAIIQDKDGYTNIRSTAGKTGAIVGKVVNGEEFYVKPTKSDWWPVR
                     TPSGVAGFMHKSRVLLR"
     gene            1489..3210
                     /locus_tag="PKO06_02955"
     CDS             1489..3210
                     /locus_tag="PKO06_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV68631.1"
                     /translation="MELGVDLLIRLRNLWNDGWFDLGFWLGRHFRKTFYLAVALIFTL
                     LAIAEVSTFQVTQEVRQQAFDAILHYRVIKPRPDPDIVIVDINEASLAAMAREYGRWP
                     WPRQVMGEFIELIQKQKPKAIVFDILFSDPDLYNSDSDTYFNDAIARTDNTFFPMLRL
                     DPNNDALSKIHASMIPGVLPPRTATTADPTIAMVLPHFRAAIDSKRLGFHNIYADDDA
                     VVRRYPVYRDELGWRLPSLPARLGGEFGWSVPDAPWVLLNWRGAPFSYQYVSFAEVFH
                     DLTRKQKHRPANEFTGKILIIGSTAPSLFDIKPTPMSQMHPGVEILATAIDNLKNGDA
                     LRYPQLETWYLLLAIAIVWVTAWGFYSGIEPVILDWWFGLSQFFMVGVSYASINLSTV
                     YINLTAPISFALAYFSVTRTYASLTRKALDPSMVRRSMRESGQRQATLLLIRCDTEAI
                     SDVGFANIRAGMLAVTHEPMSIEIIKEDQRGFWDLFEKTFAVCWMAPIEDQHARDRIN
                     EDVESVIAAFQVLLRRELVQTEGVASWFFHHGTVTCGESAEDSWRALFAETLIQWHKS
                     LATQVTS"
     gene            3207..3731
                     /locus_tag="PKO06_02960"
     CDS             3207..3731
                     /locus_tag="PKO06_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV68632.1"
                     /translation="MNQITRVLAMTVLILTTALPLLAATESAEIASGTALKPDDIREE
                     PFRDATTTGALEKGDPVSILEKDGGWLKIASEKGNGWVRLLSIRRADMTKASVTVEGI
                     LSLASGRAGTGNIVATTGIRGLTPELLRLAKFHAARLARLESYAVTRTQARKFADQGG
                     LKAVSVPYLAGDEK"
     gene            3731..4675
                     /locus_tag="PKO06_02965"
     CDS             3731..4675
                     /locus_tag="PKO06_02965"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV68633.1"
                     /translation="MRCHLSALCVMILVCLSAPLPAQDVKKAILDYFRKPSSASDTAR
                     PVLSDSDKSKLLNKLRHRLPDRLKGATDALQPISLEHEISIGRQIAGNLLGAAALVDD
                     DGLQTYINKIGRWVADQSERPELPWHFGVLDSHDVNAFAAPGGYIFVTRGLYSLLQNE
                     AELAGVLGHEIGHVIRRHHLKVLQQSRLLDLGRQIIPKKITDNAFAQSLIGQGAEIVS
                     RALDQNAEFEADRLAVILTARAGYDAFALPAVLQQIGHVSRDHDRVALLFKTHPHPDT
                     RLQKLDARLGDRLDHLKGRTLPNRLYHLKPAKTTKPGK"
     gene            4921..6072
                     /locus_tag="PKO06_02970"
     CDS             4921..6072
                     /locus_tag="PKO06_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009386236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HNV68634.1"
                     /translation="MQDRRSAALPPVRSGNSFPRLLINVRKLEHNARLMVEEFAAAGI
                     TVMGVNKVFNGLSETAEAVVRGGIQTIAESRIANLKKLAGLPVTKCLLRSPAPSEVDD
                     TVRFSDLSLESEESVLRALSAAAVRQNRTHEVMLMIDMGDLREGIWHEDRPSILRALD
                     LITTLPGLNLYGLGTNFNCYGTLLPTRENGLQFVTLARELEAARNIRFPYLSGGNCTS
                     HYLLRHGLWPEGINHLRVGGTHEFGIEYVNVEYLEGYYHSSMDVKRVCSDLYLLQAEV
                     IETNVKPTCPVGKLGRDAFLQTKTFVDRGPRRRALLAVGRQDIPHENLWPCDPGITVL
                     GQTSDHTLIDTQDAVQPPEVGEIVSFQLDYTGLLHACNAPGIEKVVTHD"
BASE COUNT         1334 a         1946 c         1638 g         1272 t
ORIGIN      
        1 gtgtttgtac gaactgccgg agctgaaacc tacgttatca cgctgacgaa agaaggtgac
       61 gagtatctgt tcgaagatat caacagcccc gacaccgatc aatgggagaa gctgcctgag
      121 ttgaaatagt tcgcaattcg caacagaaaa gaccggggaa agccttcgct cttcccggtc
      181 tttttttatt ttaccccatc tgcctgcgaa actgtgagcc agcggccatt accgctgtca
      241 cccgtcacct ggtgttcaat gtcgatcggt ctctggcgac cgtcgagacc taccgcaata
      301 agacccgact cttgtgcatg aaaccggcaa cgcccgatgg cgtgcgtacc ggccaccagt
      361 ccgacttcgt cggtttgacg taaaactctt cgccgttcac gaccttgcct acgatcgccc
      421 cggtctttcc ggccgtactc cgaatgttgg tgtagccatc cttgtcttga atgatcgccg
      481 ttctccagcc ggccggcgcc gagctttcgt ttgtccctgt cgacgcagaa acggtcggcg
      541 gctcgatctt gaccgatggg ccggaaaccg cctcatagcc acttgagccc cccggcacga
      601 aggcaaagtc ctgtgtcaga cacgacagtt cccacggacg ctgcttctgc ccggtttcat
      661 caagaacacc gactcgaaca agcttgaaca tgcgttcgac cggcatccca ggattcatca
      721 tatttttcag tatgtgcttc atgtacggag agttcccgcc gggctcacca tcggcggcgg
      781 tcttgtccgg gtcggtggca tacgcaatca gcatctgacc agcggcatcc attcgcgcca
      841 atcccatcgc cgcagatcgc gcgctgcgtt tgaacgggtt gttccggcag gcatcgagaa
      901 tgacgatatt ggtcttcgcc ttcgcgtcat ccatgaaatc gagcaacttc tgcgccggaa
      961 agcagccatg cttgatatcc atttcctttt caatcacggc attggtcggc acgagaaaat
     1021 tctcgccgtt caactgaatg ccgtggcccg aatagtagaa cagacctgct tcctgccctt
     1081 tcaaacgatt ccagaagtcg gtgatggctt catccatcgt tcgcagatcg gtatcctgct
     1141 tgaccaccac atcaaacccg acctggcgta acgcctcggc gaacgcccgc acgtcgttcg
     1201 gcggattctc gagcggcggc acgttgttgt aggccgcgtt tccgatcagg agcgccaccc
     1261 gtccgccagc ggcaaaccct gggacaaccc cgcagaccag caagacaccg agcatgaaga
     1321 cacggaatcg tttcacgaac atcatggtca aacttcctcc gcaagcggat ttcccacagt
     1381 ataagctccg gcctgcgccg acgcaacctt cccttccgta acatcgcgag ggtttgacaa
     1441 tcagcgaagc gcgtggttcc ctgacagaag acgataccga tttcacgaat ggaactgggt
     1501 gtggatctcc tgatcaggct tcgcaacctc tggaacgacg gctggttcga tctcggcttc
     1561 tggctggggc gacatttccg taaaacgttc tatctggccg tcgcactcat tttcacccta
     1621 ctggcgatcg ccgaagtctc aacgtttcaa gtgacccagg aagtccgcca gcaggcgttc
     1681 gatgcgattc tgcactaccg cgtgatcaaa cctcgccccg atcccgatat cgtcatcgtc
     1741 gacatcaacg aagccagtct cgccgccatg gcccgagaat acgggcgctg gccctggccg
     1801 cgtcaggtga tgggcgaatt cattgaactg atccagaaac agaagccgaa agccatcgtc
     1861 ttcgacattc ttttcagcga tcccgacttg tacaattctg acagtgacac gtatttcaat
     1921 gatgccattg cacggacaga caacacgttc tttcccatgt tgcggcttga tccgaacaac
     1981 gacgctctca gcaagatcca cgcgtccatg atccccggcg tgctgccgcc ccgcaccgct
     2041 acgaccgcag atcccaccat cgcgatggtg ctgccccatt tccgggcggc catcgacagc
     2101 aagcgactgg ggtttcataa catctacgcc gatgacgatg ccgtcgtgcg ccggtatccc
     2161 gtgtatcgtg acgaactggg ctggcggctg ccttccctgc ccgcacgcct gggcggcgag
     2221 ttcggctggt cggttcccga tgctccgtgg gtcttgctga actggcgcgg agcaccgttc
     2281 tcctaccagt atgtgagctt cgccgaggtg ttccacgatc tgaccagaaa acagaaacac
     2341 cgacctgcga acgagtttac cggcaagatt ctcattatcg gctcgacggc accaagcctc
     2401 ttcgatatca aaccgacccc catgagccag atgcatcctg gagttgagat tctggcgact
     2461 gccatcgaca acctcaagaa tggagacgcg ctccgctatc cacagctcga gacctggtat
     2521 ctgctgctgg ccatcgccat tgtctgggtg accgcctggg gcttctacag cggaatcgag
     2581 cccgtgatac tggactggtg gtttggtctg tcccagtttt tcatggtcgg agtgtcctat
     2641 gcgagcatca acctgagcac cgtttacatc aatctcaccg caccgatttc gtttgccctc
     2701 gcctacttca gtgtgacccg cacctacgcc tctctgacga gaaaggcact cgacccgagt
     2761 atggttcgtc gctcgatgcg cgagagcgga cagcggcagg caacgcttct tctgattcgt
     2821 tgcgacaccg aggcgatcag tgatgtcgga ttcgccaaca ttcgcgccgg catgctcgcc
     2881 gtgacacacg agcccatgag tatcgaaatc atcaaggaag atcagcgcgg attctgggac
     2941 ctcttcgaaa aaaccttcgc cgtctgctgg atggcaccga tcgaagatca gcacgccaga
     3001 gaccgaatca acgaagatgt cgagtcggtg atcgcggcat ttcaggtttt actgagacga
     3061 gaacttgtac agacagaggg tgtcgcctcc tggttcttcc atcatggcac agtcacatgc
     3121 ggcgagtcgg ccgaagacag ctggcgtgcg ttgtttgccg aaactctgat acaatggcac
     3181 aaatcactgg ccacgcaggt gacatcatga accagatcac tcgtgttctc gctatgacag
     3241 tactgattct cacgaccgcg cttccgctgc tggcagcgac agagtctgca gagatagcca
     3301 gcggcaccgc cctcaaacca gatgacattc gcgaagaacc gttccgcgat gcgaccacga
     3361 cgggcgccct ggaaaaggga gatccggtga gcatcctcga gaaggacggt ggctggctga
     3421 agatcgccag tgaaaaggga aacggctggg tgcgcctgtt gagcattcgt cgcgcggata
     3481 tgaccaaggc gtccgtcacc gtcgagggaa ttctctcact cgcttcgggg agagccggaa
     3541 ccgggaacat cgtcgccacg accggcattc ggggcctgac tcccgaactg ctgcgcctgg
     3601 cgaaatttca tgccgcacgg ctggccagac tcgagtcgta tgccgtcacc cgcacccagg
     3661 cccggaagtt tgccgatcag ggcggtctca aggccgtctc cgttccgtat ctggcaggag
     3721 acgaaaagtg atgcgatgcc acctctccgc cctctgcgtc atgattctgg tctgcctgag
     3781 cgcccccctt ccggcgcagg atgtcaagaa agccatcctc gactactttc ggaagccctc
     3841 atcagccagt gacacagctc gtcccgttct ttccgactcg gacaaatcaa agttgttgaa
     3901 caaactgcgt catcgacttc ccgaccgttt gaagggggca accgacgctc ttcaaccaat
     3961 ttcactggaa catgaaatct cgatcggcag acaaatcgcc ggcaacctgc tgggcgcggc
     4021 ggcgttggtc gacgacgacg gcctgcagac gtatatcaac aaaatcggcc gctgggttgc
     4081 tgaccagagc gaacgccccg agcttccctg gcactttggc gtcctcgact ctcatgacgt
     4141 caatgctttt gccgctccag gaggctacat cttcgtcact cgcggcttgt actctctact
     4201 tcagaacgaa gcggagctgg caggagtcct ggggcacgaa atcggccacg tgattcgccg
     4261 gcatcacctc aaggtgttgc agcagagccg tttgctcgac ctcggccgcc agatcattcc
     4321 caaaaagata accgacaatg ctttcgcaca aagtctgatc ggccagggag ccgagatcgt
     4381 ctcgcgcgcc ctcgaccaga acgccgaatt cgaagccgat cgccttgccg tcattctgac
     4441 ggctcgtgcc ggctacgacg ccttcgccct gcccgccgtt ctgcagcaga tcggccacgt
     4501 ttcccgagac cacgaccggg tcgccctgct cttcaagacg caccctcacc ccgacacccg
     4561 cctgcagaaa ctcgacgccc gcctcggcga ccgtctcgac cacctgaaag ggcgaacgct
     4621 gcccaaccgg ctctatcatc tgaagcccgc caaaaccaca aaacccggca agtaatcccc
     4681 gcggcctgaa gcgccaacca cgcgggttca caacatcgga caagtggcga tcgcatcacc
     4741 tggagaaacc ctcaccgagc aggccacgtg aaatacactc tggcgaaaca ctcagcttcg
     4801 acaatcaaag cacgggggtc gagtcgactc cgtctgtact ggcagcctct gctctgcctc
     4861 tccagaaaac tcgaatgaaa atacgaacca caaggtcccg aaaacctgta ttatagggcc
     4921 atgcaggatc gccgctctgc cgctctccct cccgtccgat cgggtaactc cttcccgcgt
     4981 ctgctgatca atgttcgcaa gctcgagcac aatgcccgac tgatggtcga ggagttcgcc
     5041 gccgcgggca ttaccgtcat gggcgtcaac aaggtcttca acgggctgag cgaaaccgct
     5101 gaagccgtcg tgcgcggggg catccaaacc atcgcggaat cacggatagc gaatctgaaa
     5161 aagctggcgg gtctgcccgt gacaaagtgc ctgctccgaa gtcccgcccc cagcgaagtc
     5221 gatgataccg tgcggttttc tgacctttcc ctcgagtccg aggaatccgt gctgagagcg
     5281 ttgtcagcgg cagcggtgcg ccagaaccgc actcacgagg tcatgctcat gatcgacatg
     5341 ggcgatctgc gagagggcat ctggcacgaa gaccgtcctt cgatcctgcg agcactcgat
     5401 ctgataacga ctctccctgg actgaacttg tacggactcg gaaccaattt caattgttac
     5461 ggcacgttgc ttccgacccg tgaaaacggt cttcagtttg tgactctggc ccgcgaactt
     5521 gaagccgcca gaaacattcg tttcccctat ctttccggcg gaaattgtac cagtcactat
     5581 ctcctgcgcc acgggctgtg gcccgaggga atcaaccatc tgcgagttgg cggcacccac
     5641 gaattcggaa tcgaatacgt caacgtcgag tatctcgagg gctactatca ctcgagtatg
     5701 gatgtaaagc gcgtctgttc cgacctgtat cttctgcagg ccgaggtgat cgaaacaaac
     5761 gtcaaaccga cgtgtccggt cgggaaactt ggccgggacg cgtttctcca gacgaaaacg
     5821 tttgtcgatc gcgggccgag gcggcgagcc ctgctcgcgg tcggccgcca ggacattccg
     5881 cacgaaaacc tctggccgtg tgacccggga atcaccgttc tcgggcagac cagcgatcac
     5941 acgctgattg acactcagga tgccgtccag cctcccgaag tcggtgaaat cgtctcattt
     6001 cagcttgatt acaccggcct tctgcacgca tgcaatgcgc ctggaatcga aaaagtggtg
     6061 actcatgact gaaccactgc gcgaatctcc cgcgaccccc gaaacagaaa atcctgggcc
     6121 accaccacag tggctgatga tagggctggc cattggcctg aatctgctct gggcattctc
     6181 gtatccggtc
//
LOCUS       DAORHD010000085         6184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12732_length_6184_cov_4.062979, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000085 DAORHD010000000
VERSION     DAORHD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12732_length_6184_cov_4.062979"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..2024)
                     /locus_tag="PKO06_02975"
     CDS             complement(30..2024)
                     /locus_tag="PKO06_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HNV68635.1"
                     /translation="MNSGLIIEYLSKDTGLAIGMVLSVQPKGIRILTTGKRETTIQER
                     SILVSGPALTVNLNDLDGCLELLQQADRRRVELEPVVHLEELHALLVEEPRIYGLAEM
                     AEYLGAARDDHLQAALLRRLKHDALYFREKKDGWTPIPVAEVQTALEKERSRLAREES
                     DRQLAEELHQLCLSPTTTYSDRVREVLPLIEQIAVWKTEAKVPQRIFDLLEQAGIGWN
                     QKLAQFLIDTGRFQPDENLLLRKHNVPRTFGSEALREAEQLVMTPHSPENRVDLRHLR
                     TWVIDNAETRDRDDGFSIHAELDGSITLWVHIADGAHWISLGSSLNKEASRRGTSIYL
                     PDDKLHMLPPTLSEEHLSLNQGTDRTCLTQRLRFSVDGNLLESEFCESLVHISDAISY
                     AQADVLSANEAEIQLGLRLAELLRLRRRQQGGVILTRPELTVKLRDGEILVERRMGRG
                     DSQEMVAEFMIWANHHAAAWCRERHVPCLYRSQEPPENPPEMPETFDPVLFYRAIRSL
                     RRGAISVEPTLHHGLGVGLYCQVTSPLRRYNDLLLQRQIKAALHGQPTPYSSESLMQT
                     ILWAENATGTGEEIMNQRHRYFLLKYLKQKLDRGEERFEATVVDLSQDCTFFNHDTCE
                     FARTRKPTFSVQVGQKLLMRYLAVEPAENQLRYEVLQPLE"
     gene            2059..2625
                     /locus_tag="PKO06_02980"
     CDS             2059..2625
                     /locus_tag="PKO06_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HNV68636.1"
                     /translation="MERQETAPGVPPFPLARLISGGQTGVDRLALDVAMSLGIPVGGW
                     CPRGRRAEDGVIPERYPLREASSELYQERTRLNVRDSDVTLILCSGAPSGGTLLTIEM
                     ARTMHRPFYLADMFTASDEQLIDVLRRWLWDIRPSTLNVAGPRFSEWPQLEPQVTCLL
                     RRCVVASSLPPPVWPPARPMTPSLPGTS"
     gene            2768..4360
                     /locus_tag="PKO06_02985"
     CDS             2768..4360
                     /locus_tag="PKO06_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68637.1"
                     /translation="MWYNQFCTEPMENYYLILGIPAFSPLEDVQTAYNRQFGELFVSE
                     SPLANLPRMKLLKEALEILSDPVRREEYDARLKTFLESLEKKFGDAVDRLSEGKFDEA
                     IALLRECLKINPREPDFYETLGLAFQLRGTLDEALKAFQQGLQLKSKNAQFNWYIADI
                     YRQMRDDERFETYLLDAAEGFKEILKTDPRNVHAQEMLADCYGKMRFFDEAVEVYNKL
                     IEMFPYKAEYRRDLGALLYEMDCLDDAQESLVEALRNAPEDASSYLYLGLVYYKKRLL
                     AWAIQNLDESLKRNPQQPEVRSLLEKIREVQKDVGRTVEEIINDPTPDAMVEGSVKWY
                     NSETGIGVLSCPEYAEVLLHYTALKPEDVDALKKGDAVRFGVVKDQVGLIAVQVERLE
                     DGQVGDTLPGKISKFDHKRKLGMISVPDGREIMFTFAALSGELAADKLEIGLDVLFDA
                     KTIRGLNDDLHEQAMNVRPRKKPTVKPIGTPKGPAAVVPSSPAPAPAPSPESPPPSQP
                     PTPEPTSEPPARKGGRKPKKGS"
     gene            4459..5823
                     /locus_tag="PKO06_02990"
     CDS             4459..5823
                     /locus_tag="PKO06_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68638.1"
                     /translation="MPVNRRPKLTLIMPSIGRKPDQRYIATWQMEPLVLAVLAALTPP
                     DWEITIQDDRLVPVDTTQPADLVGITVETYTAKRAYQIAAEYRRRGVKVVMGGYHPTL
                     WTDEVLEHADCVVTGPAEGIWGKVLADVSAGCLQKRYDGPFDFAWKGLFPRREVYADK
                     NYLPLALVEFSRGCRFHCQFCAITAFTRGRHYYRPPSDVAEEIRRSGQKIVFLIDDNI
                     AANHEAAFALCEALKPLGVSWVSQISVDAAADGRLVRAMAEAGCIGVLIGFESLRADL
                     MQRLNKKCNGSIELYERALAQVRINGICVYAAFLFGIDGDDRGAFPRTFRFIQKHRFF
                     LAAFNHLVPFPGTPLYARLEAMNRLTSPAWWLDSEFRFGYVPFHPQEMSAQELAETCR
                     HWRRRFYSWRSIIHRALDARTNARRLSTLAMFLTQNFFGRREVDEKYGLPLGMIGSDQ
                     KFPV"
     gene            5846..>6184
                     /locus_tag="PKO06_02995"
     CDS             5846..>6184
                     /locus_tag="PKO06_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68639.1"
                     /translation="MNLSAPYELTTWRAGSPSSPADEQLDARIRHLLSACHMPTPILS
                     LAYQCEPSFRRALACQGDEHYLVYARDRAADELNSLGIRSLRTSSLNQVACRMAYIHH
                     LRIHPQQAGGT"
BASE COUNT         1271 a         1689 c         1829 g         1395 t
ORIGIN      
        1 cgaagaacca gcgagaggcg ggtgccatcc tattcgagcg gttgaagcac ttcataacgc
       61 agctggttct ccgcgggttc aacggcgaga taacgcatca gcagtttctg accgacctgt
      121 accgaaaacg tgggttttcg ggtgcgggcg aactcgcagg tgtcgtggtt gaagaatgta
      181 cagtcctggc tgagatcaac gacagttgcc tcaaatcgct cttctccgcg gtcgagtttc
      241 tgtttcagat acttgagaag aaaataccga tgtcgctggt tcataatctc ctctccggtt
      301 cctgtcgcat tttcggccca gagaatcgtc tgcatgagag actcagagga atacggcgtc
      361 ggttgtccgt gcagggcggc cttgatctga cgctgcaaca gcagatcatt atagcgcctc
      421 aacggcgaag tcacctgaca gtacaagccg acacccaagc cgtgatgcag cgtcggttcg
      481 accgagatcg ccccgcgtcg caatgagcga atcgcacgat agaaaagtac cgggtcgaag
      541 gtttccggca tctcgggcgg gttctccggt ggttcctgac tgcgatacag gcagggaaca
      601 tgacgttccc gacaccaggc agccgcatga tgattggccc agatcatgaa ctcggccacc
      661 atctcctggc tgtccccacg ccccatgcgc cgttccacga ggatttctcc gtcacgcagt
      721 ttcacggtaa gttccgggcg ggtgagaatg acgccaccct gctggcgacg tcggagtcgc
      781 aggagctcgg cgagccgaag tccgagttgt atttcggctt cattggctga gagaacatct
      841 gcctgggcat aggaaatggc gtcagagatg tgaaccagcg attcacagaa ctccgactcc
      901 agaaggttgc cgtcgacaga gaagcgaagt cgttgggtca ggcaggtgcg atccgttccc
      961 tgattcagac tcagatgttc ttctgaaagg gttggcggaa gcatgtgcag tttgtcatcg
     1021 ggtagataaa tggaggtgcc gcggcgtgag gcttctttgt tcaggctcga cccgagcgag
     1081 atccagtgcg cgccgtcggc aatgtgaacc cagagggtga tcgatccgtc gagttcggca
     1141 tgaattgaga aaccgtcatc ccggtcgcgg gtctcggcat tgtcgatgac ccaggtgcga
     1201 agatgacgca gatcaacgcg gttttcggga gaatgaggcg tcattacgag ttgttcggct
     1261 tcgcgcagag cttcggagcc gaaggtgcgt ggcacattgt gtttacgtaa aagcagattt
     1321 tcatcaggtt gaaaacggcc ggtgtcgatc agaaactggg cgagcttttg gttccagccg
     1381 attcccgcct gttcaagcag atcgaagatt cgttgcggaa ccttcgcctc ggttttccac
     1441 accgcgatct gctcgatcag tggcagaacc tcacgcaccc ggtcggaata ggtcgtcgtc
     1501 ggtgacagac agagttggtg caattcctcg gcaagctgac gatcagattc ttcgcgggca
     1561 aggcgcgaac gttccttctc gagagcagtc tgaacctctg cgactggaat gggggtccag
     1621 ccgtccttct tctcgcgaaa gtacaaggcg tcgtgcttga gtcggcgaag gagcgccgct
     1681 tgcagatggt cgtcacgagc tgctcccaga tactcggcca tttcggcaag accgtaaatg
     1741 cggggttctt cgaccagcaa ggcatgcagt tcctcgaggt gcacgacagg ttccagttcg
     1801 acccgtcgcc gatcagcctg ttgcagaagc tccagacatc cgtcgagatc gttgagattg
     1861 accgtcagtg ccggcccgct caccaggatc gatcgctcct gaatcgtggt ttcgcgcttc
     1921 cccgtggtga gaatgcgaat gcccttgggt tggaccgaga gtaccatccc gatggccaga
     1981 cctgtatcct tgctgagata ttcgatgatg agtccggagt tcataatctg tgcggggcat
     2041 tgtaacatac tgtttgttat ggaaagacag gaaaccgcgc cgggtgtgcc gccgttcccg
     2101 ttggctcgtc tcatctcggg cggccagacc ggcgttgatc gacttgcgct cgacgtggcg
     2161 atgagtctcg gtattccggt gggcggttgg tgtcccaggg gacgtcgagc agaagatggt
     2221 gtcattcccg agcggtatcc actgcgcgaa gcgtcgtcgg aactttacca ggagcgcacc
     2281 cggttgaacg tgcgggattc ggatgtgaca ttgatcctgt gttccggagc gccctcgggc
     2341 ggaacacttc tgacgatcga gatggcgcga accatgcatc gcccatttta tctggcagac
     2401 atgttcaccg cctcagatga gcaactgata gacgtcttgc gacgctggct ctgggacatt
     2461 cgaccgtcaa cattgaacgt ggctggacct cgcttcagcg aatggccgca actggaaccg
     2521 caggtcacgt gcctgctgcg gagatgcgtc gttgcgtcgt cgttgccgcc acccgtctgg
     2581 cctcccgcgc gacccatgac cccatcgctt cccggcacat cctgacttcc gacacccgag
     2641 ccgcgtcaaa cgttcttcgt ttcgggcctg gtttcaggca gtctgacggg gacagttcgc
     2701 gagatcacgc ctcgaatttg gagaggctgg agtgtctgtc tccttcacga tctcctgagg
     2761 agaatgagtg tggtataatc agttttgtac cgaacctatg gaaaactatt atctgattct
     2821 gggcattcct gccttttcgc ctctcgaaga cgttcagacg gcatataatc gccagttcgg
     2881 tgaactcttt gtctctgaaa gtcctctcgc gaaccttccg cgcatgaaac tgctcaagga
     2941 agcgctcgaa atcctttctg atcctgttcg gcgcgaggag tatgacgcgc gcctgaagac
     3001 gtttctcgag tcgctcgaga aaaagtttgg agatgcggtt gatcggctct cggaaggaaa
     3061 gttcgatgaa gcgatcgcgc tgctgcgtga atgtttgaaa atcaacccgc gtgaacccga
     3121 tttctatgaa acgctggggt tggcctttca gctgagaggc acgctcgatg aggctttgaa
     3181 ggcgtttcag caggggttgc agctgaagag caagaatgcg cagttcaact ggtacattgc
     3241 cgatatctat cgacagatgc gtgatgatga gcggttcgag acctatctgc tcgatgccgc
     3301 cgagggcttc aaggaaatcc tcaaaaccga tccgcgcaac gtgcatgcgc aggagatgct
     3361 cgccgactgt tacggcaaaa tgagattctt cgatgaagcg gtcgaagtgt ataacaaact
     3421 gattgagatg tttccgtata aagctgagta tcgacgcgat ctgggtgcgc tattgtacga
     3481 aatggattgc ctcgatgatg cgcaggaaag cctggttgaa gcactgagaa atgcccccga
     3541 ggatgcgtcg tcctatctgt atctcggcct tgtctattac aagaagcggc tgctggcctg
     3601 ggccattcag aatcttgacg aatcgctcaa gcgcaacccg caacagccgg aagtccggag
     3661 tctgctcgaa aagattcgcg aggtgcagaa ggatgtgggt cgtacggtcg aagagatcat
     3721 caacgatcct acgcctgatg ccatggtcga gggatcggtg aaatggtaca attccgagac
     3781 cggcatcggc gtcctcagct gcccggagta tgcggaagtg ttgttgcact ataccgcgct
     3841 gaagcccgaa gacgtcgatg cactgaaaaa aggcgatgcc gttcgcttcg gggtggtgaa
     3901 agaccaggtt gggttgatcg ccgtccaggt tgaacgtctc gaagacggtc aggtgggcga
     3961 caccttgccc ggaaagatct cgaagttcga tcacaagcgc aagctgggta tgatttccgt
     4021 gcctgatggg cgtgagatca tgttcacctt tgccgctttg agcggcgaac tggccgcgga
     4081 caaactcgag attggtctcg atgtgctgtt cgatgccaaa acgattcgcg gtctgaacga
     4141 cgatcttcat gagcaggcga tgaatgtgcg accacgcaag aagccgaccg tgaaaccgat
     4201 tggaaccccc aaggggccgg ccgcagtcgt tccctcatcg cctgcccccg ctcctgctcc
     4261 cagccccgaa tcgccgccgc cgtcccagcc accgactcct gaacctacgt cagagccccc
     4321 ggcacgcaag ggtggtcgca agccgaagaa aggctcatga cgagtcgtac accccgaaaa
     4381 tggtctgagc gccatttttc gctgtgcatg cgagaaatga caccgtgaat cgagcgtgac
     4441 acggtgctgg aggaactgat gccagtgaat cgtcgtccga agctcacgtt gatcatgccg
     4501 tcgattgggc gtaagccaga tcagcgctat atcgccacct ggcagatgga gccactggta
     4561 ctggccgtgc tggcagccct gaccccgccg gattgggaga ttaccattca ggatgaccga
     4621 ttagtcccag tagatacgac gcagcctgcc gatctggtag gcattaccgt tgaaacgtat
     4681 acggcgaagc gggcatatca gattgccgcc gaatatcgcc ggcgtggcgt gaaggtcgtg
     4741 atgggcggtt accaccccac cctctggacc gacgaagttc ttgaacacgc ggattgcgtc
     4801 gtgaccggtc cggcggaagg gatctgggga aaggtgctgg ccgatgtttc cgccggttgc
     4861 ctgcagaaac gctacgatgg tccattcgac ttcgcgtgga aggggttgtt tccgcgtcgc
     4921 gaggtctacg ccgacaaaaa ttatctgccg cttgccctgg tcgagttttc gcgtggctgt
     4981 cgatttcact gtcagttttg cgcgattacc gcgttcacgc gtggtcgtca ttactatcgt
     5041 ccgccgtctg acgtggccga agagattcgc cgcagtggtc agaagatcgt ctttctgatc
     5101 gatgacaaca ttgccgccaa tcacgaggcg gccttcgcgc tctgcgaagc tttgaaaccg
     5161 ctgggggtca gctgggtgag tcaaatcagt gtcgatgcag ccgcagatgg ccgcctggtg
     5221 cgggccatgg ccgaagcagg ctgtatcggg gtgctgatcg ggttcgagtc gttgcgcgca
     5281 gatctcatgc aaagactcaa caaaaagtgt aatggttcga tcgaattgta cgagcgagcg
     5341 ttggcgcagg tgcgcatcaa tggtatctgc gtctatgctg ctttcctttt tggcatcgac
     5401 ggcgacgatc gcggtgcctt tccgcgcacc ttcaggttca ttcagaagca tcggttcttt
     5461 ctggccgcat tcaatcatct ggttccgttt ccgggaaccc ctctctatgc ccgcctggaa
     5521 gccatgaacc gactgacgtc gcctgcctgg tggcttgatt cggaattccg ctttggctac
     5581 gtcccctttc atcctcaaga gatgagcgct caggaactgg ctgagacgtg ccgtcactgg
     5641 cgacgtcgct tctattcctg gcggtcgatc attcaccgcg cactcgatgc tcgtacaaac
     5701 gcccggcgct tgtcgaccct ggcgatgttt ctcacgcaaa acttcttcgg tcgtcgtgaa
     5761 gtcgatgaaa agtacgggtt gcctcttggt atgatcggtt cagatcaaaa gtttccggtc
     5821 tgagatcgcc agacatgcct gtcgcatgaa tctctcggcg ccgtacgaac tgacaacctg
     5881 gcgcgcgggg tctccttctt cacccgcgga cgaacagttg gatgcgcgga taaggcatct
     5941 gttgtccgcc tgtcacatgc ccacgccgat tctgtcgctg gcctatcagt gcgagccttc
     6001 gtttcgacgc gcgctggcct gtcagggcga cgaacattat ctcgtctatg cgcgtgatcg
     6061 cgcggctgac gagttgaatt ctctcggcat tcgatcgctg cgtacaagtt ctctgaacca
     6121 ggtcgcgtgt cgaatggcct atattcacca cctgcgcatt catccgcaac aggcgggtgg
     6181 cact
//
LOCUS       DAORHD010000086         6175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12763_length_6175_cov_4.096895, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000086 DAORHD010000000
VERSION     DAORHD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12763_length_6175_cov_4.096895"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKO06_03000"
     CDS             complement(<1..489)
                     /locus_tag="PKO06_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68640.1"
                     /translation="MSRVARLTVCLLVFLGFFGVVSPTVAQEASITASGTIVRYDIAS
                     GLPSRYPRALTWTSTGEIIAAFMAESGVNQWLGAATLNPASATGHLLTTRGLESLHVT
                     SLAASGTTVLATTERGLYRLEPDAWRRIGPSWHWQSLAPDDDGAFWVAGRDPQGRLAV
                     GRM"
     gene            complement(486..1571)
                     /locus_tag="PKO06_03005"
     CDS             complement(486..1571)
                     /locus_tag="PKO06_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV68641.1"
                     /translation="MNTPVFQPQSIWFCAEGWEAGLSEELSRKFPGRTFERLDSGVVG
                     CKGFHPPRPGAEIFPRFFLSEAHLIEASSHREIIRQAGPLVDAALDHTHDRWTLHVST
                     PGVFTLDGEHYREASARAGLLADLFLERMRTYRKRAMERFTAADAFRQGLVAQFWVLS
                     SQRVIVTVTRERLARKMTLSTLFLPAASQIPADAKAPCRSYYKLEEAFLHAHRSPRPG
                     DTCVDLGAAPGGWSWGALKRGARVTAIDAADLAEHVASHPNCVHRRDNGFEYLPVQPV
                     DWMFCDMIVKPLAALGLLERWLQAQACRSFVLNIKFRGREPGSFLPDIERLQHTYELG
                     RLKIRHLYYDRSEITLIHTHHREGDIR"
     gene            complement(1568..4135)
                     /locus_tag="PKO06_03010"
     CDS             complement(1568..4135)
                     /locus_tag="PKO06_03010"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68642.1"
                     /translation="MNTRKSAADISDWIEAHRRTLPLGGAWLLGGEPNLQPAGQFASA
                     RLRVLIARLSSYHDVAAGITHSFLWQLAKDVPGTFVDLAYFPSAAEERAFAADAVPLW
                     FGTTTREPAPRFDILAISHSVVQELLNLPAALANSGIALDAVTREQQGAPLVILGGSN
                     SYATAILHGSSGLTSHAGLIDVLLIGDAEEAWPAFLRLYHQRRGTERQAFLREAARTI
                     PGCLVPALYQEEYDENGVLSSISVTDNAPFPVRAARADLARAAGFREGPLFYDETTAG
                     SSHLLISAGCPYFCSFCKESWEQKPYRERQLDTLIETASQLKANLGLREVSLMTFNVN
                     TITTLYQLLTQLEPQFERVALKSQRFDAIAHAPHLLERQLEAGKRTYTCAMEGISDRL
                     RRFLQKGLPETLLLEGFRQLLQRNLRQMKVFIIVTGREQEADRVEFAALLANLKAQMA
                     HLRGKPLITFSLAALFRPPFTPLQFEPPRDQAGALETWMRRIEETVKKGGFECRISAG
                     PWDALVSEYLAYADRRSTPILVKASLEKRFRYRGEVERAVYVWWQQELRERFTRLPFP
                     PEPRTDATVFPWSLVDTGVTQTALYRHYQLLLSGQEQASCIAQPLGSGTCHGCGACPD
                     PATRRRLTSLEIAPPRTARPCVAATPRSFRLFGRIARRLAPVGNRFLEAAFSRLLMRG
                     AQLVFPDLTAGFGEFRASLPRGGAFGLFVGDFTIRCSTQPPWDSIIPQANAQSSDLEI
                     LQIVSASGSIDQLIREERESVIHLHAHRQTDAIARDIDHLLQKYRFKHQKRWENEILT
                     WQIAPGQAKKCGFSQVRLPRHGGTLTVTFLCEPEPHLLAFFLGLGDREIRIPPEVSR"
     gene            complement(4159..5304)
                     /locus_tag="PKO06_03015"
     CDS             complement(4159..5304)
                     /locus_tag="PKO06_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68643.1"
                     /translation="MVEKAELQIMPDGRFRFVALRRGKPGLWTRGSVRRMPGVTSTGW
                     DFLCEDLHGTLRGEVVKAALDLIWQPDPLSVERYRFDPVAALPAFESGHPAVLPAQPW
                     VATPLDDSSEPPRTWSLWRGLLAASADLPRGTGRTVTGSTLLFLDNDRQIAGVTLFPS
                     AIPGSPLVLLPLTADLHVSLDRQAAALNWSTPKPAPSYQSCVISGQQPEPGKAWTGET
                     SFGGLVRSTFRFEPIGNGVSTPFSTPAVPTRTYSLVAALEQSDPTFPLPVRRLFPLSI
                     SFTDNRLWFSCPDDTGFRLAVPFELSAAGRLVINGDAQTGHLLRLADRDVAALVIRSV
                     AGSSPGFQSRFIDLDHLWLVLDLTVQRFHPVPRSHHCRLALYLRWSS"
     gene            5347..5520
                     /locus_tag="PKO06_03020"
     CDS             5347..5520
                     /locus_tag="PKO06_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68644.1"
                     /translation="MTAVDAAERVNRRQDARREKYDQADEGQNAKCRGNVDRRWRATG
                     GVTGSYRREGDVP"
     gene            complement(5525..5659)
                     /locus_tag="PKO06_03025"
     CDS             complement(5525..5659)
                     /locus_tag="PKO06_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68645.1"
                     /translation="MDCKNCPDRLFCYTAPTTWPISDQRESAAILLRVLRCRKGLARR
                     "
     gene            complement(5738..>6175)
                     /locus_tag="PKO06_03030"
     CDS             complement(5738..>6175)
                     /locus_tag="PKO06_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68646.1"
                     /translation="SDPDSEPPALPEELASLSLWLSHLRTLQSNAEEIWHVGKAGYAQ
                     HPRVKILQVTSLKTLDDIKGYESMNWDKASDWISRARVLGEQFRTLYWSLSRWVLEKS
                     STWVDRLPPLTLRTRHNLETLEGDYLILEATIASETGRSRNPK"
BASE COUNT         1278 a         1800 c         1994 g         1103 t
ORIGIN      
        1 catgcggccg acagccaggc gaccctgtgg gtcccggccg gcaacccaga aggccccgtc
       61 atcatcaggc gcaaggctct gccagtgcca ggacggacca atgcgtcgcc aggcatctgg
      121 ctcgagtcga tacaggccac gctcggtcgt cgccagcacg gtggtgcctg atgccgcgag
      181 agatgtcacg tgcagagact ccaggccccg cgttgtcagc aggtgtccgg tcgcactggc
      241 cgggttcagc gttgcggccc ccagccactg gttcaccccc gattcggcca tgaaggcggc
      301 gatgatctcg ccggtagatg tccaggtcag ggctcgcgga tatcgtgatg gcaagccgct
      361 tgcgatgtca taacgaacaa tcgtgccgct ggcggtgatc gatgcttcct gcgcaacggt
      421 cgggctgacg acgccgaaga aacccagaaa gaccagcagg caaaccgtca gacgtgccac
      481 ccggctcatc gaatgtcacc ctcgcgatga tgggtatgaa tcagcgtgat ctcactgcga
      541 tcatagtaca aatgccggat tttcagtcgg cccagttcat aggtgtgctg caaccgctcg
      601 atgtcgggca gaaaggaacc gggctcgcgg ccgcggaact tgatgttcag gacgaatgaa
      661 cggcaagcct gcgcctgcag ccagcgttcc agaaggccca gcgcggccag gggtttgacg
      721 atcatgtcgc agaacatcca gtcgaccggc tgcacgggca gatactcgaa cccgttatcg
      781 cgacggtgaa cgcagttcgg gtgtgacgcg acatgttcgg ccagatcagc ggcgtcgatg
      841 gcggtgaccc gggcgccgcg tttgagcgcc ccccacgacc agccgccagg agccgctccc
      901 aggtcgacac aggtgtcgcc ggggcggggg cttcggtgag catgcagaaa agcttcctcg
      961 agcttatagt acgagcgaca gggagccttg gcatctgccg gaatctgaga agcggcgggc
     1021 agaaaaaggg tcgagagagt catcttccgc gcgagtcgct cacgggtcac cgtcacaatg
     1081 accctctggc ttgacaggac ccagaactgc gcgaccaagc cctggcgaaa ggcgtctgcc
     1141 gcggtaaaac gctccatggc gcgcttgcga taggtacgca tgcgttcgag aaacagatcg
     1201 gccagaagtc cggcgcgggc tgaagcctca cgatagtgct cgccgtcgag cgtgaagaca
     1261 cccggcgtcg agacgtgcag ggtccagcga tcgtgtgtgt gatctagtgc cgcatcgacc
     1321 agcggcccgg cctgacgaat gatctcgcgg tgcgaggaag cctcaatcag atgagcttcc
     1381 gacaaaaaga atcggggaaa gatctcggct cccgggcggg gcgggtgaaa tcccttgcag
     1441 ccgacgacgc cagagtccag gcgctcgaag gtgcgtccag ggaacttccg actcaactcc
     1501 tctgacagcc cggcttccca gccttcggca cagaaccaga tcgattgtgg ttggaaaacg
     1561 ggggtgttca tcgggatacc tccggtggaa tgcgaatttc gcggtcgccg agcccgagaa
     1621 agaacgcgag caggtgtggc tctggctcac acagaaacgt gacggtcagc gtgccgccat
     1681 gtcggggcag gcgtacctgc gagaagccgc attttttcgc ctgtccgggc gcgatctgcc
     1741 atgtcagaat ctcgttttcc cagcgcttct gatgcttgaa tcgatacttc tgcaacaggt
     1801 ggtcaatgtc gcgggcaatc gcgtcggttt gacgatgtgc gtgcagatga atcaccgatt
     1861 cacgttcctc cctgatgagc tggtcgatag agcctgatgc agaaacaatc tgaagaatct
     1921 caagatcgga cgattgagcg tttgcctgcg ggatgatgga atcccagggc ggctgtgtac
     1981 tgcatctgat ggtgaagtcg ccgacgaaca gcccgaaggc tccgcctcga ggcagcgagg
     2041 cgcgaaactc accgaacccg gcggtcagat cagggaacac caactgtgcg ccccgcatca
     2101 acaagcgcga gaatgcggct tcgagaaaac gattgccgac cggcgcgagc cggcgggcaa
     2161 tccgcccgaa cagacgaaag gagcgcggcg tcgcggcgac gcagggccga gccgtgcggg
     2221 gcggggcgat ctcgaggctg gtcaggcgac gacgggtcgc cgggtcgggg caggctccgc
     2281 atccgtgaca tgtgccgctg cccaatggct gagcgatgca gctcgcttgc tcctgcccac
     2341 tgaggagcag ctgataatga cgatacaatg cggtttgcgt gaccccggta tcgaccaggc
     2401 tccagggaaa gaccgtcgcg tcggttcgcg gctcaggcgg aaacggcagg cgggtaaaac
     2461 gctcgcgcaa ttcctgctgc caccagacat agacggctcg ctccacctcg ccccgataac
     2521 gaaagcgctt ttcgagagaa gccttcacga gaatcggggt cgatcgccga tcggcatacg
     2581 ccagatactc cgagaccaga gcgtcccagg ggccggcgct gatacggcat tcaaagccgc
     2641 ctttcttcac ggtttcctcg attcgtcgca tccaggtttc cagcgctccc gcctgatctc
     2701 gcggtggctc gaactgcaga ggcgtgaacg gggggcgaaa cagcgcagcc agcgaaaagg
     2761 tgatcagagg cttgccgcgc agatgagcca tctgtgcctt cagattggcg agcaaagctg
     2821 cgaattcgac ccgatcggcc tcctgctcgc gtccggtcac gatgatgaag actttcatct
     2881 ggcgcagatt tcgttgcagc agttggcgaa acccttccag cagcagtgtt tcgggcagac
     2941 ctttctggag aaaccgtcgc agtcggtcgc tgattccctc catggcgcag gtgtaggtac
     3001 gtttgcctgc ttcgagctgc cgttcaagaa gatgcggagc gtgtgcgatg gcgtcgaagc
     3061 gttggctctt cagggcgacc cgctcgaact gcggctcgag ctgggtaaga agctgataca
     3121 aggtggtgat ggtgttgacg ttgaacgtca tcaggctgac ttcacgcaat cccaggttgg
     3181 ctttcaactg cgaagctgtc tcgatcaggg tgtcgagctg ccgttcacga tatggcttct
     3241 gttcccacga ttccttgcag aacgaacaga agtagggaca ccccgctgaa atcagcagat
     3301 gcgaagagcc ggcggtcgtt tcatcataaa acagcggtcc ctcgcgaaat ccggcggcgc
     3361 gggccagatc agcgcgggcg gcacgaacgg gaaacggagc gttatctgtg actgagatgc
     3421 tagataaaac gccattttcg tcatactctt cctggtacaa agctggaacc agacacccgg
     3481 gaatcgtgcg cgccgcctct ctgagaaagg cctgccgctc ggtgccgcgt cgctgatgat
     3541 acaggcgaag aaacgcgggc caggcttctt cggcatctcc gatcagaagg acatcgatga
     3601 gccccgcgtg cgacgtgagg ccggaactgc cgtgcagaat cgcggtggcg taactgttgc
     3661 tcccgcccag aatcaccagc ggagcaccct gttgttcccg cgtgacggca tcgagtgcga
     3721 tgcccgaatt cgccagagcg gcaggcaggt tcagcagttc ctgcacgacc gagtgactga
     3781 tggcgagaat gtcgaaccgg ggtgccggct cgcgggtggt cgtcccaaac cagagaggta
     3841 ccgcatcagc ggcaaacgcg cgttcttcgg cggctgacgg aaagtaggcg agatcgacga
     3901 acgtgcctgg cacgtctttt gccaactgcc agagaaagga atgggtgatg ccggccgcga
     3961 catcgtgata cgacgagagg cgtgcgatca gaacccgcag tctggctgag gcgaactgcc
     4021 cggcaggctg caggtttggt tcgccgccca gcagccaggc acctccgaga ggaagcgtgc
     4081 gtcgatgcgc ttcgatccag tcggatatgt cagccgcgga cttccgggta ttcacgtttc
     4141 atgcttaaca tagaaccgtc atgaactcca gcgcagatac agcgcgaggc gacagtggtg
     4201 cgaacgtgga accgggtgga accgctggac ggtcagatcg aggaccagcc acaggtgatc
     4261 gaggtcgatg aaccgcgact gaaatcccgg tgatgaacca gccacggagc gtatgaccag
     4321 cgcggcgaca tcgcgatctg cgaggcgcag cagatgtcct gtctgggcgt caccgttgat
     4381 cacgagtcgt ccggcagcgg aaagctcgaa gggaacggcc agccgaaacc cggtgtcgtc
     4441 tgggcacgag aaccacaggc gattgtcggt gaaactgatc gagagaggaa acaaccgccg
     4501 taccgggagt ggaaaggtgg ggtcgctctg ctcgagtgcc gcgacgaggg aataggtgcg
     4561 agtggggact gccggcgtgg aaaagggtgt gctgaccccg ttcccgatcg gttcgaagcg
     4621 gaacgtgctg cgtaccagtc cgccaaagct ggtctcgccg gtccacgctt ttcccggctc
     4681 gggctgttgt ccactgatca cgcaactctg atacgaggga gcgggtttgg gagtcgacca
     4741 gttcagagcc gcggcctgcc ggtcgagact gacatgcaga tcggctgtca ggggcagcag
     4801 gaccagcgga ctccctggaa tcgctgatgg gaaaagggtg acaccggcaa tctgtcggtc
     4861 attgtccagg aacaacagag tcgagcctgt gacggttcgc ccggttccgc gcggcagatc
     4921 agcagacgcg gccagcagac cgcgccacag gctccaggtg cgcggtggct ctgacgaatc
     4981 gtcgagtggt gtcgccaccc acggttgcgc cggcagaacg gccggatgac cgctctcaaa
     5041 cgccgggaga gccgcaaccg gatcgaaccg ataacgctcg acggagagcg gatcaggttg
     5101 ccagatgaga tccagtgctg ctttaacgac ttctccgcgc agcgtgccgt gcaggtcttc
     5161 acacagaaag tcccagccgg tactcgtgac acctggcatc cggcgaacgc tgccgcgggt
     5221 ccagagtccg ggtttccccc gccgcagagc gacgaagcgg aaccgcccgt ccggcatgat
     5281 ctgcagctcg gccttctcga ccatctgagg cacctcgcga tcgagttcgc gcgtgaaaat
     5341 gtaccgatga ccgctgtaga tgccgctgag agggtgaacc gccgccagga tgccagacgg
     5401 gaaaagtatg atcaggcaga cgagggccag aacgcgaaat gccggggcaa tgtcgacagg
     5461 agatggcgcg cgaccggtgg cgtgaccggc agctaccggc gcgagggaga tgttccctga
     5521 atcgtcagcg cctcgccagg ccctttcggc aacgtaacac gcgcagaaga atggcggctg
     5581 attcgcgctg gtcagagatt ggccaggtgg ttggtgcggt gtagcagaac aggcgatcgg
     5641 gacagttctt gcagtccatg cctttctcct tggtcgtgtt cgcgtgttga tgttccatcc
     5701 ctccccttat cggaagattc aacgatttga ctgagcgtca cttggggttt cgagaccggc
     5761 cggtttccga ggcgatggtc gcctcgagaa tcagataatc gccctcgagc gtctcgagat
     5821 tgtgtcgcgt gcgcagcgtg agcggaggaa gccgatcaac ccaggtcgaa gacttctcga
     5881 gcacccagcg gctgagcgac cagtagagag tacgaaactg ctcgccgagg actcgggccc
     5941 gtgagatcca gtcactggct ttgtcccagt tcatcgattc gtatcctttg atatcatcga
     6001 gtgttttcag cgacgtaacc tgcagaattt ttactcgtgg gtgctgagca taccctgcct
     6061 tgcccacatg ccagatctcc tcggcgttgc tctgtaacgt tcgcagatga gacagccaca
     6121 gggaaaggga cgctagttct tccggcagcg ctggtggttc actgtccggg tcgct
//
LOCUS       DAORHD010000087         6162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12809_length_6162_cov_3.666121, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000087 DAORHD010000000
VERSION     DAORHD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12809_length_6162_cov_3.666121"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2201
                     /locus_tag="PKO06_03035"
     CDS             <1..2201
                     /locus_tag="PKO06_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68647.1"
                     /translation="RVRTVCTHVYRPPKPRQLPLSDPLEPRVPFPVRANLCPGSGAFV
                     HEITFSKLVPLPLTERVLPPPREEVASLFLRRWNLETRVQPIAPAPRAWVPGPVHCPP
                     LRTEGPRWVAHPLAVRTFTTEAVTRLTTTLARHLALTARAARPELWRVPLPPRMAAPV
                     QRQSGAFAAPFRQPRRQGFLPPPAHVVTMRGLAIDERSSPIFAPPVETHHVPAPFNTT
                     IRLEYIHHTADLFLEQVPWHRAPQCPPFTEPPTVQVTHRFSVDPVLLEYRFDFALVDS
                     DDGISSLLKRARFSPAAWPFSPLPTICEFLSFVSSHPSKLSLNRPRYSQVFGHVGERL
                     REQPYLTSWPRRLRCRSIFQNPSAFSLHRAFIGSQRGLGLPFSMLTQSLPRPVLTRRA
                     LKTLLHVEPAAVPSLPQPILPEFTRLPVAAPPAPTPAFSPPLDTVPKASSPRPTLAEI
                     RKQGLGRSVLSGTLTHTLRENSYASVAPLPAGSGAYFGYTPREEWFHLNLSGFVLLVA
                     DSPGAQALADSPVTHLRMPPYRSAPVVTGTITHLSIPPLSHDRAPFHHLIEDLPAVRP
                     TAPLFSLLGTHFAALLLIDGHMHASGVDQRPFRPAIVPLSPAPVVYRSPQLALTDHGE
                     RIPLGKPANHILELLYHILVDRLRHHRLRLEVTENRQRQILGTRRSGQGHKPTAQLRD
                     RFFSLTPANLSAPPRITQPAARNLRVKMARLAGLLETAQKLNALVGPTPP"
     gene            4379..>6162
                     /locus_tag="PKO06_03040"
     CDS             4379..>6162
                     /locus_tag="PKO06_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68648.1"
                     /translation="MRLRPLELVLSLASPFYPLVVDCRSIRVTSGNLSPKRFFRHFSG
                     SGVREPRRWPVPGCLIARIRIPFQEAVPFEIAAALPGARFPRELWPDPGTNPFTQLPF
                     HMEPRNRNQGFRSTFRLHPPIVICRWGNLDRLPQEASGSRDRETPSLRGTIMPSKRSL
                     VAAYRCDAGSSLSFTRFQASLTEAQPVRASFSEEVDVWSVLYRSLPRRQKPKFDKPRL
                     IESGGRCLARFKSLTYPWRPAIEPLRGETCLLPLSPPESAPTLEDLVSQPFSIVPLFH
                     ETFSETMPHRVGWVSASHPSPRTFPGSIWVSPRLSWSLHAFSLTRGAPRRPRLDRLPQ
                     GSQSNVPAARLRFQFQSRRERPLFISASPTSAVHPASLLLLHDPALPIRRLAQSAPHF
                     VPETQSLDPGSHTRPLAFPVPFRTQDRDFWCTLLELDAPVTRQHKLICPSLHIAGPRV
                     PGRLKPTDLQMGPLRVRFCPIYRSASLATHEALPLDVSTTPARLPAATPVSREACVLL
                     QRPERTSAWAPHPPQENNVRDRCVFALEAAPRQRAPLRLTHPGPRHPSLGINAFGLPD
                     WIDLPPWQRPLPGDLALTADFRQPSGPDG"
BASE COUNT         1012 a         2264 c         1560 g         1326 t
ORIGIN      
        1 cccgcgtcag aacagtttgc actcacgttt accgcccgcc caaacccaga caactccccc
       61 tgtccgaccc actcgaacca cgggttccat tccccgtgcg cgcgaatctc tgccccggct
      121 ctggcgcttt tgttcacgag attacttttt ccaaactcgt accattgcct ctgaccgaac
      181 gtgtcttgcc gcccccccgg gaagaagtgg caagcctttt tctgcgtcgc tggaatctgg
      241 aaactcgcgt gcaaccgatc gctccagccc cgcgcgcttg ggttcccggg ccggtacact
      301 gccccccgct gcgcaccgag ggtccgcgct gggtcgccca tcccctcgcc gtcagaacct
      361 tcacgaccga ggctgtcacg cgactgacga cgactctggc acggcatctc gctctcaccg
      421 cccgagcggc ccggcccgag ttgtggcgtg tcccgctgcc cccccgcatg gccgcccccg
      481 ttcagcgaca gtcaggagcc ttcgccgcgc cattccgtca gccgagacgt cagggattcc
      541 tgccaccccc ggcgcacgtg gtgaccatgc gcgggctcgc gatagacgag cggtcttccc
      601 cgatcttcgc gccgccggtt gaaacacatc acgtgccggc tcctttcaac accacgattc
      661 gtcttgagta catccaccat acagccgacc tgttcctcga gcaggttccc tggcatcgcg
      721 ctccgcaatg cccccccttt accgaaccgc cgacagtaca agtgacacat cgatttagtg
      781 ttgatcccgt gcttctcgaa taccgattcg attttgcact cgtcgattct gatgatggca
      841 tcagctcgct cctgaaacgc gcaaggttct cccctgcagc atggccattt tctccgctgc
      901 ccaccatctg cgagtttctc tcctttgttt cttcacatcc aagcaagcta tcactcaatc
      961 gcccccggta ctctcaggtg ttcggtcatg tcggcgagag gctgcgcgag cagccatatc
     1021 tcaccagctg gccccggcga ctgcggtgcc gatcgatttt tcagaacccc agtgcctttt
     1081 cgttgcatcg cgcgttcatc ggttcacagc gtggtctggg actgcccttt tccatgctga
     1141 cccagtcgtt gcctcgtccg gtgctgaccc gacgcgccct caagacgctg ctgcatgtgg
     1201 aaccagccgc ggtcccgtca ttgccgcaac cgattctgcc ggaattcacc cgcctgccgg
     1261 tcgcggcgcc cccagcaccg acgccagcgt tttctccccc gctcgataca gttcccaaag
     1321 cttcttctcc acgacccact ctcgcggaga ttcgcaaaca gggcctgggg cgttccgttc
     1381 tcagtggcac cctgactcac accctgcgcg aaaactcata tgcatcagtg gccccacttc
     1441 cggcaggcag tggagcgtat ttcggctata ccccacgaga agagtggttt catctcaatc
     1501 tcagcggctt cgtgctgctg gtcgccgatt ccccgggtgc gcaggctctg gccgactcgc
     1561 ccgtcacgca tcttcgcatg ccgccctatc ggtcggctcc ggttgtcacc ggcacgatca
     1621 ctcacctgtc gattcctccg ctgagtcatg accgagcgcc atttcatcac ctgatcgaag
     1681 atctgccggc cgttcgcccg accgcgccat tgttctcgct tctgggaacg catttcgcag
     1741 ctctgcttct gatcgatggc cacatgcacg caagcggggt cgatcagcgc ccgtttcgcc
     1801 ccgccatcgt ccccctgtcc cccgcaccgg tcgtctatcg atcaccgcaa ctggctttga
     1861 ccgatcatgg cgagcgaata ccattgggca aaccagccaa tcacattctg gaactgctgt
     1921 atcacattct ggttgaccga ttgcgtcatc atcgtctgcg cctcgaggtc accgaaaacc
     1981 gtcagcgcca gattctggga acacgacgat ccggccaggg acacaagccg accgcccaac
     2041 tgcgcgaccg cttcttctcg ctcaccccgg ccaacctttc cgctccgccc cgaatcacgc
     2101 aaccagcagc gcggaatctg cgcgtaaaga tggcgcggct cgccggcctg ctggaaacgg
     2161 ctcagaaact gaacgctctg gttggcccca ctccgccatg aacgtcggtc atcacgcaat
     2221 tcccgttttt cacgtctggt catcagcttc agacgcgaat aagccgattc gacgacttgg
     2281 acacgtacgc ctcttcggtc tggcaagcca gttgcgcatc agcagtggtc ccgaaccttc
     2341 gcaccggcgc gtcttctgtt tctgccccga tgtattctct ctcttcgcgg ggttctcgct
     2401 tcgtcgaccg cagccggtcc tggtcagtct gccctgtcgc ctgatcgaaa aaccctgtgc
     2461 gttcgccaca gctgctcccg cgaccccttt cctgtcggtt caccgtcttg gccatgagcg
     2521 tacccgtctt ccggtgcgtc agcagctcca gttgtgcgtg ccagagtcga cgttggccga
     2581 ggaactcgca cgggcgagca ccgcgatctc ttttcaaccg ctcgccgtct ctcacctgag
     2641 cacgtatgtg tcgcgacctg catctctcat cgcgccgaca cccagacgtg aagcagtgcc
     2701 acttcgtgtg tcaacgtcgc tgcgaaccca agtcggtgat cccgaccctc tccccgccgt
     2761 tcaaaccccg tcgttgacga ggcttgaccg cgagatgtgg cccgttcaca gcgtttccgc
     2821 gctgaagcgc atctcatcgc tggccagagc cgcggcaccg acagtcatgg cgctcccgac
     2881 cagacgtcat ctggcgtttc ctgcgccgcg aaatcgattt cagaccttac gtgaatttcg
     2941 cttcggcgag cccctcggct atcctgcctc gctctttccc gcacgggttc ggcgtgaacg
     3001 tctcgccctg gctaccatcg acctcgccgg tcggcacccc ctggcacaga tgctcgtttc
     3061 gcgggcggta ctcgccccga tctgccgccg ccctcgcccg cagccccagg tcattctccc
     3121 accacttccc cggtcaggcg acgttctcgt gtttttgccg gtggctatcg gtcatgaacc
     3181 tcgccagccg ttcctcctcc cgggacggag gcaggttcca tcgcgtctcg aatgtcgtga
     3241 gcgggcgctc caggcgcgcc tcgccacggt attaccgccc cagtgcctgc caaccatgcc
     3301 gactgaccgg caacactggc ccacccattg gagtgccagc atctggaagc agttacgtcg
     3361 actcaagctg cacgcgcgca cgctccggtg cccccgccgc tggttcgaac gaccgttctt
     3421 cttcaacgtg ctcggcttcc tgccgccgcc actcacgatt gaattgaaac cagcgcccct
     3481 cacacgtggt tcggtcggac cgtctccatc gtccgatcgt cccacacatc gcgacttttc
     3541 accgggtcga ccgcgatctc tcgcggtaac gccctgtgct tatgtcgact ccggtaccca
     3601 ctacaccttt cagacacgcc atgttccgcc accgctgccg cagacggtcg acggattcgt
     3661 gctgacacgg cagcggctcg aaccgtgggg tgccatgaca catcccagac cgtacacacg
     3721 ctttttccca ttcgcgcaaa gctccagatc gcttatggcg caatttcaac gctcagagat
     3781 ctggtactct cccaccgttc acggccgtat cccatggcag gccatctcgt ggattcgcga
     3841 gcgggcgcaa tccatggtgt tcctcacgac tcctcttttc atccctgcca ggcaaccacc
     3901 tctccccagg cgcatccgca gcatatccgg gacgtatcga catctccccg ggtatatgca
     3961 gcgtcaacga cggcgcacgc tgattgccta tttcttcact cgcgaaaggt cttttcgagc
     4021 cgactacatt gatcacagac cggtacgaaa aaagattgcc tgcgaacgtc gggctttgcc
     4081 catttcccca cctcgaaccc atggtgtcca cactgtcctt aagcggagac aggatcgacg
     4141 cgaagtgctc ccggcaccgt tcgctgccgc accttccgcg cctcacgcgt cgttattcat
     4201 cgagcggctc ccgctcgctc cggtcttttc acgattgcgg cagcccttcg gtttgccgcc
     4261 atcgcccaga gatcgtctcc ctgcacggtt tcgcgtccct ctggatctgc acctccgatc
     4321 aacaacgcag ccccgtctgg aaatcccgct cggagcgttc cccttcggat tcgaaccgat
     4381 gcgcctgcgc ccgctcgagc tcgtactgag cctcgccagc ccgttctatc ccctggttgt
     4441 cgattgtcgc tcgattcgcg tgacttccgg gaatctgagt ccgaaacggt tctttcgcca
     4501 cttctcgggc tcaggggtgc gcgaaccgcg tcgatggccc gttccgggtt gtctgatcgc
     4561 acgcatccgg attccgttcc aggaggcggt tccgtttgaa atcgccgctg ccctccccgg
     4621 agcccgtttt ccccgcgagt tgtggccaga ccccggtacc aacccgttca cccaacttcc
     4681 cttccacatg gaaccgcgaa atcgcaacca gggtttccgc tccacgtttc gcctgcaccc
     4741 ccccattgtc atctgccgct ggggcaacct ggatcgtctg ccgcaggaag catctggttc
     4801 cagggaccgc gagacgccgt ctctgcgagg gaccatcatg ccctcgaaac gctcgctggt
     4861 tgccgcgtat cgatgcgacg caggcagctc tctttctttc acccgttttc aggcttctct
     4921 caccgaagct caaccggtac gggcgagttt ttctgaagaa gtcgatgtct ggtccgtgct
     4981 ctatcgttcg ctcccgcgac gccagaagcc aaagttcgac aaacctcgcc tgatcgaatc
     5041 tggcgggcgg tgtctggcac ggtttaaatc gctgacgtat ccctggcgac cagccatcga
     5101 accgcttcgc ggtgaaacct gcctgctccc cctgtcaccg cccgaaagcg caccgactct
     5161 cgaagatctc gtcagccagc cgttttccat cgttcccctg tttcacgaga cgttttcaga
     5221 gaccatgccg catcgagtcg gttgggtcag cgcgagtcac ccctccccgc ggacttttcc
     5281 tggctcgatc tgggtatcgc cccgactgtc ctggtcactt catgccttct cgcttaccag
     5341 aggcgcacct cgacgcccac gtctcgatcg gttgccccag ggatcgcagt cgaacgtccc
     5401 ggcggcgagg ctgcgttttc agtttcaatc acgtcgagaa cgccccctgt tcatcagcgc
     5461 ctcgccgact tcagccgttc acccggcctc gcttctcctg ctgcatgatc cggcacttcc
     5521 catacggcga ctggctcaaa gtgctccgca cttcgtgccc gagacccaga gtcttgatcc
     5581 aggctcacac acacgtcccc tggcgtttcc ggttccgttt cgcacgcagg accgcgattt
     5641 ctggtgcacc ctgctcgaac tcgacgcccc agtgacacga cagcacaagc tcatctgtcc
     5701 gagtctgcac attgccggac ctcgtgttcc cggtcgtttg aagccaacag atcttcaaat
     5761 gggacccctt cgcgtcagat tctgcccaat ctatcgcagt gccagcctcg cgacgcacga
     5821 ggcactccct ctcgatgtgt ccacgactcc ggcacgcctg cctgcggcca ctcccgtgag
     5881 tcgcgaggct tgcgtcctgt tgcaacgccc cgagcgcaca agcgcctggg caccgcatcc
     5941 acctcaggaa aacaacgtgc gtgaccgctg cgtctttgcg ctcgaagcag caccccggca
     6001 gcgcgctccg ctccgtctga ctcacccggg tccacggcat ccctcactcg ggatcaacgc
     6061 tttcggactt ccggactgga tcgatctgcc accctggcag cggccactcc ccggggatct
     6121 cgctctgact gcagacttcc ggcagccgtc tggtccggat gg
//
LOCUS       DAORHD010000088         6140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_12874_length_6140_cov_3.458340, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000088 DAORHD010000000
VERSION     DAORHD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12874_length_6140_cov_3.458340"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2077)
                     /locus_tag="PKO06_03045"
     CDS             complement(<1..2077)
                     /locus_tag="PKO06_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68649.1"
                     /translation="MEEKSLQHLEFPKVLALIAGFAGSKAAKEAILALRPITDRLQIV
                     RGLDELDELLGFLDRGYHLPISGLREMRDLVEQLQGGRDVLDGDEFLRIRSNLEVIRE
                     VRRSFQDAPAMPLLQARLDSLPDLSPVHREISLTISDKGAVRDDASPRLAGIRRDLLK
                     ARQELEKRLTDYLHSQGDTFSDSFFTLRNERYVVPVKVGSQSQVQGIVHDESGSGQTL
                     FMEPLEFLAGNNRLARLKSEEREEVLRILRALTQLLATHLSGLRHTFDTLVFFDQLHA
                     RARFARAYAAHRPEIAADGSLELIQARHPLLHPNCVPLDLKLGGTTSCLVITGPNGGG
                     KTVGLKITGVNALLMQCSCFILASVNSRLPIFDHVLSDIGESQSIEEHLSTFTSHLQR
                     LNEILRDAGPRSLILMDEIGVGTDPQEGAALAQGVLKALVRRGCLTLVTSHYHALKHL
                     AFTTPGFQNGCMEFDEETHRPTFRFLAGASGRSNALAMARQFRLPEEVLAEMVQSIEG
                     RGGDEIKLLEGLERERAHAENLRRSLQERDRQLEQRQKDLEQELQKLSQFRRTKRDEL
                     TESFEREFKDRLKQLEALIHALRQPQPGLSAEAALDQARAALQETKRAIETLEQEKAE
                     VQSEKPEILDQPPDIGDDVLWPRFNLRGKLEALENNGKQAIVETNGKKTSVPFAELQA
                     IRASRRSGPG"
     gene            complement(2089..3633)
                     /gene="cobA"
                     /locus_tag="PKO06_03050"
     CDS             complement(2089..3633)
                     /gene="cobA"
                     /locus_tag="PKO06_03050"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV68650.1"
                     /translation="MNTSVVPRNHHTPPGTVYLIGAGPGDPGLITVRGRALIETADVI
                     VYDQLGAPVFLTLARPDAELIDVGKRAGRHTVPQPEINRLLVEHAQRGRQVVRLKGGD
                     PMVFGRGGEELDALVDAGIPFEVVPGVSSAIAGPSYAGIPLTHRDCTTALAIATAHEA
                     DLKEESTIPWSSLSGINSTVFLMGVAQLANIVDHLLRQGRNTTTPIAIIENATTPRQR
                     TVTGTLADIVNRAHEAAVKPPALIVVGEVVRFHDRFNWFEKRPLFGRSIAVTRASAQA
                     ATMADRLQALGAEVVLSPAITIHPIDPNPLFESFLHEINSYRDVVFTSVNGVEVFFNQ
                     LRQRGKDARLLAGHSIICIGPATGDACRQHGIIPDFIPETFVAESLLPWFQNRPPART
                     ALLRAESARDLLPDGLRRHGCQVNVIPLYRTDFVPQAGADLLTRLRDNTIDAVTFTSS
                     STVDAFCRWLAATDLDRRTIPAVAIGPVTADTCRERGLQPIAEASVHTIPGLIDLLLQ
                     RFGRTS"
     gene            complement(3723..4661)
                     /gene="hemC"
                     /locus_tag="PKO06_03055"
     CDS             complement(3723..4661)
                     /gene="hemC"
                     /locus_tag="PKO06_03055"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006193692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNV68651.1"
                     /translation="MKTLRLGTRGSRLALVQARHVQQILQKSIPDLHIEEVIIRTLGD
                     NVTGVPLFQVGGQGLFIREIERALQSQDIDLAVHSLKDMPHALGEGLTLAAVTTRADA
                     RDVLISRAGESLAQLASGARIGTSSLRRQAQLLSLRPDLSFVALRGNLDTRLKKLEAG
                     EADAIVLAAAGLERLGLSAHCTQFFSFDELTPAAGQGALGLECRSADLELLTPILAPL
                     IDVSSQTAAQAERAFLAAVEGGCQVPLGVHATLDNSTLFIRACIGDPRGCRIVRKTIS
                     GPATQAAELGRTIAAHLLDQGGREILGTLRRPPDDR"
     gene            complement(4658..5989)
                     /gene="hemA"
                     /locus_tag="PKO06_03060"
     CDS             complement(4658..5989)
                     /gene="hemA"
                     /locus_tag="PKO06_03060"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNV68652.1"
                     /translation="MSLLLIGISHHTAPVEIREKLAPLCTSGLPGADGRSIEAVTVFT
                     CNRVEVYLAGDVVEGERAFQQWLTDCGCPWSELGTHFYRRVDIEALTHLLRVASGLDS
                     LVLGENQILHQLKDSYQAAIQAGSVGKQLHGLFQKALETGKRVRAETRISENTVSVAS
                     TAVSLARQIFGHLHNCTALLIGAGEMATLVARHLHTQGIGRLIFTNRTSSRAEELARQ
                     FSGEAAPVERLAELLVQADLVITSTGSPRPILGRREFSAALERRPFRPLFAIDIAVPR
                     DIAPDCEDLDNLYVYNIDDLQMVVDQNLDLRRIESEKAEAIVRYEAGSYQTVIEAFSA
                     APLIRALRQQAESLRVAEVERLLTRFPEWSAEQRDAVDQATRALLAKWLHQPILALKE
                     RSRAGREELLELARLFGLSEGVVPQIPLSLVSENREKDASAEKQTVIKDTP"
BASE COUNT         1238 a         1715 c         1956 g         1231 t
ORIGIN      
        1 tgccaggtcc cgagcggcga gaggcgcgaa tggcctgcag ctccgcgaac ggcaccgacg
       61 tctttttgcc gttggtttcg acgatcgcct gttttccgtt gttttcaagg gcttcaagtt
      121 tgccgcgcag gttgaagcga ggccagagaa cgtcgtcgcc tatatctggc ggttgatcaa
      181 gaatctctgg cttttcggat tgtacttctg ccttctcctg ctcgagcgtc tcgatggcac
      241 gcttggtttc ctgcagtgct gctcgtgcct ggtcgagggc ggcttcggcc gagagtcctg
      301 gttgcggttg tcgcaaagcg tgaatcagtg cctccagctg cttgagacga tctttgaact
      361 cgcgctcgaa actctcggtt aactcatcgc gtttggtgcg gcggaactgt gacagcttct
      421 gcagttcctg ttcgagatct ttctgacgct gttcgagctg acggtcgcgc tcctgcagcg
      481 aacggcgcag attctcggcg tgtgcgcgtt cgcgttcgag accttcgaga agcttgatct
      541 catcaccacc acgaccttcg atcgattgta ccatttcggc cagaacttcc tcgggcaatc
      601 ggaactgcct ggccatcgcc agtgcgttgc tgcgacccga agcgccggcg agaaagcgaa
      661 aggtggggcg atgcgtttct tcatcgaatt ccatacagcc gttctggaag cctggcgtgg
      721 tgaatgccag atgcttgaga gcatgatagt gtgacgtgac cagagtcaga cagccacggc
      781 gtaccagtgc cttgagcacc ccctgagcca gggctgcgcc ttcctgtggg tcggtgccga
      841 cgccgatctc gtccatgaga atcagtgagc gtgggccggc atcgcgcagg atctcgttga
      901 ggcgctggag atgcgaggtg aaggtcgaca ggtgttcttc gatgctctga gattcgccaa
      961 tgtctgacag cacgtggtcg aaaatcggca gacgcgaatt caccgaggcg agaatgaagc
     1021 agctgcattg catgagcagg gcattcactc cggtgatttt caagccgacg gtttttccac
     1081 ccccgttcgg cccggtaatc accagacagc tggtggtgcc gccgagcttg aggtcgaggg
     1141 gcacgcagtt cgggtgcagc agtggatggc gcgcctggat caactcgagt gacccgtcgg
     1201 cggcgatctc gggacgatga gcagcatagg ctcgtgcaaa gcgggctctg gcgtgcagtt
     1261 ggtcgaaaaa gacgagggtg tcgaacgtgt gacgcaatcc gctcaggtgt gtggccagca
     1321 gctgggtcag cgcgcgcaga atgcgcagga cctcttcacg ctcctcggac ttcaagcgag
     1381 cgagacgatt gttgccggcg agaaactcga gtggttccat gaaaagcgtt tgccccgacc
     1441 cggactcgtc gtgcacgatg ccttgaacct ggctttggct gccgactttc acaggaacca
     1501 cataccgctc gtttcgcagg gtgaagaaag agtcggaaaa cgtgtcgccc tgagaatgca
     1561 gatagtcagt cagccgtttc tcgagttctt gacgggcttt gagcagatcg cgacgaattc
     1621 ccgccagccg aggagaggca tcatcacgca cggcaccctt gtcggaaatg gtcagcgaaa
     1681 tttcccggtg aaccggcgac aggtcgggca ggctgtcgag tcgcgcctgg agcagaggca
     1741 ttgctggcgc atcctggaac gaacgcctca cttcccgaat gacttcaaga ttcgagcgaa
     1801 tgcgcagaaa ctcgtcaccg tcgaggacat cgcgaccgcc ctgcagctgt tcgaccagat
     1861 cgcgcatttc acgcagaccg ctgatcggca gatgataccc gcgatcgaga aagccgagca
     1921 gctcatcgag ctcatcgagc cctcggacaa tctgcaggcg atccgtgatc gggcgcagcg
     1981 cgagaatagc ctccttggcg gccttggaac cggcaaagcc ggcgatgagc gcgagtactt
     2041 tgggaaactc gaggtgctgc agcgactttt cttccatgat cgtgaaactc aggaggtacg
     2101 cccgaatcgc tgcagaagca gatcgatgag gcccggaatc gtatgaacgg aagcttcggc
     2161 aattggttgc agaccccgtt cgcgacaggt atcagcggtg acgggaccaa ttgccacggc
     2221 gggaatggtg cggcggtcga gatcggtggc ggcgagccag cggcagaagg cgtcgacggt
     2281 cgagctgctc gtgaaagtca cggcgtcgat ggtgttgtcg cgcaggcgag tcagcagatc
     2341 ggccccggcc tggggcacga aatcggtgcg atacagtggg atcacattga cctgacagcc
     2401 gtgtcggcgc aggccgtcgg gcaaaagatc gcgtgcgctc tcggcgcgca gaagggcagt
     2461 acgcgcgggt gggcgattct gaaaccacgg tagcaggctt tcggcaacga aggtttcagg
     2521 aatgaagtcg ggtatgatgc catgctgtcg gcaggcatct ccggttgccg gcccgatgca
     2581 gataatcgaa tgccctgcca gcaggcgcgc atctttcccg cgttgccgca gctgattgaa
     2641 aaacacctca acgccgttca ccgacgtgaa caccacgtct cggtacgagt tgatttcatg
     2701 aagaaagctc tcaaaaagcg ggttggggtc gatcggatgt atggtgatgg cggggctcaa
     2761 cacgacttcg gcccccagcg cctgcagacg gtcggccatg gtcgctgcct gggcgctggc
     2821 gcgagtgacg gcaatgctgc gtccgaacag gggacgtttt tcaaaccagt tgaaacgatc
     2881 atggaaccgc acgacttcgc cgacaacgat cagagctggt ggcttgacgg ccgcttcatg
     2941 ggctcgattc acgatgtcgg ccagagttcc ggtcacggtg cgttggcgcg gggtcgtggc
     3001 gttctcgatg atcgcgatgg gggttgttgt gtttcgtcct tgtctgagca gatgatcgac
     3061 gatgttggcc agctgggcaa cccccatgag aaacacggtg ctgttgatgc cgctcagtga
     3121 actccacgga atcgtcgact cctctttgag atctgcttca tgagcggtgg caatggctaa
     3181 cgcggtggta caatcgcggt gtgtcagcgg gatgccggca taggacggac cggcaatggc
     3241 ggagctgact cccggaacaa cttcgaaggg gattcctgcg tcgaccaggg cgtcaagttc
     3301 ttcgccaccc cgaccgaaga ccatggggtc gccacccttc agtctcacca cctgtctgcc
     3361 gcgctgggcg tgctcgacca gcagacgatt gatctcgggc tgcggaaccg tatgccgacc
     3421 cgcgcgtttc ccgacgtcga tcaattcggc gtcgggtcga gccagcgtaa gaaagacggg
     3481 tgcgcccagt tggtcgtaga caatcacgtc cgcggtttca atgagcgcac ggccgcgtac
     3541 ggtgatcaag ccggggtctc cggggcctgc tccgatcagg tagacggtgc cgggaggggt
     3601 gtggtggttc cgtgggacaa ctgaggtatt catgatggtg agccgcatgg taacacgaaa
     3661 tgcgcgaaca cgcgagagcg tcgcgagggg aataccctgg cgaaattggc tctatgaaat
     3721 gatcatcggt cgtccggggg gcgacggagc gtgcccagaa tctcgcgacc accctgatca
     3781 agcaggtgag cggcgatggt tctgccgagt tcggcggcct gcgttgctgg gccggaaatc
     3841 gtctttcgta cgattcgaca acctcgggga tctccgatgc aggcgcgaat gaagagtgtc
     3901 gaattatcaa gggtcgcatg cactcccaag gggacctgac atcccccctc gacggcggcg
     3961 agaaacgcac gttcggcttg agcggccgtc tggctggaaa catcgatcaa gggggcgaga
     4021 atgggagtga gcaactcaag gtccgcggag cgacactcaa gaccaagtgc gccttgtccg
     4081 gcggccgggg tcagttcatc gaaggaaaag aactgagtgc agtgggcgga tagtccgagt
     4141 cgttccagtc cggcagcggc gagaacaatg gcatcggcct caccagcttc gagcttcttc
     4201 aggcgagtgt cgaggttgcc gcgcagggcg acaaaggaaa gatcgggtct gagagacagc
     4261 agttgtgcct gtcggcgcag actcgaggtt ccaatgcggg ctccagaagc aagttgcgcc
     4321 agcgactcac cggctcgact gatcagtaca tctctggcat ccgcacgcgt ggtcacggcg
     4381 gctaacgtca gtccttcgcc cagggcgtgg ggcatatctt tcagactatg taccgcgaga
     4441 tcgatatcct gagactgaag cgcgcgttcg atctcacgaa tgaacaaccc ctgaccacca
     4501 acctgaaaca gaggcactcc ggtgacgttg tcgccaaggg ttcgaatgat cacttcttca
     4561 atatgaagat ctgggatact cttctgaagg atctgttgca catgccgagc ctggacaagc
     4621 gccagtcggc tccctcgggt gccaagccgc agcgtcttca tggggtatcc ttgatgacag
     4681 tctgtttctc tgccgacgca tctttttccc tgttctcgga aacaagtgag agcggtattt
     4741 gcggaaccac gccctcagag agtccgaaga ggcgggcgag ttcgagcagc tcttcgcggc
     4801 cggcgcgaga tcgttccttg agagccagga tcggttgatg cagccacttg gccagcaggg
     4861 cccgggtggc ttgatcgacc gcgtcgcgtt gctcggcgga ccactcagga aatcgagtca
     4921 gcagacgctc gacttcggcc actcggagac tttctgcctg ttgtcgcaac gcgcgaatca
     4981 gcggtgcggc tgaaaaggct tcgatcacgg tctgatacga ccccgcctcg tagcgcacga
     5041 tggcttcagc cttttcgctt tcgatgcggc gcagatcgag attctggtcg acgaccatct
     5101 gcagatcgtc gatattgtac acatacaggt tatcgagatc ctcgcagtcg ggagcgatgt
     5161 cacgcggcac ggcgatatcg atggcaaaaa gcggccggaa agggcgtcgt tcgagagccg
     5221 cggaaaactc gcgccgcccg agaatcggtc ggggcgaacc ggtagaggtg ataaccagat
     5281 cagcctgaac cagcagttcc gccaggcgtt ccactggcgc agcttctcca gaaaactgcc
     5341 tggccagttc ctcggcgcga gagctggtgc gattggtgaa gatcaggcgt ccgatgccct
     5401 gagtgtgcag atggcgggca accagagtgg ccatttctcc ggcaccgatg agcagagccg
     5461 tgcagttgtg caagtggcca aaaatctgac gggccagact cacggcggtc gaagcgactg
     5521 aaaccgtgtt ttccgaaatg cgggtctcgg cgcgaacccg ttttcccgtt tcgagggctt
     5581 tctgaaagag gccgtgcagc tgtttgccga ccgacccagc ctggatcgcg gcctggtatg
     5641 agtccttgag ttgatgcaga atctgatttt ctccgagtac cagcgaatca agcccggaag
     5701 ccacacgcag taagtgcgtg agagcctcga tatcaacacg ccgatagaaa tgggttccca
     5761 gttcggacca agggcagccg cagtctgtca gccactgttg aaatgctcgt tcgccctcta
     5821 ccacgtcacc tgcgagatac acctcgaccc gattgcaggt gaagaccgtc acggcttcga
     5881 tgctgcggcc gtcagcgcca ggcagacccg aggtgcacag cggggccagc ttttcccgaa
     5941 tctcgacggg agcggtatga tgactgatgc cgatcaggag cagagacatt gctcgatctc
     6001 cctggcgacg gtctgaattc cttcggtgcg aatgcgttcg accgtgtgtt ctgtttccca
     6061 gcgacgatga aacgcctgtc gttcgtcggg gtcggaaaac gtgcgacgaa tacgctcgcg
     6121 caggcggttc atgagtgttg
//
LOCUS       DAORHD010000089         6097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13028_length_6097_cov_3.758524, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000089 DAORHD010000000
VERSION     DAORHD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13028_length_6097_cov_3.758524"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2655
                     /locus_tag="PKO06_03065"
     CDS             <1..2655
                     /locus_tag="PKO06_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV68653.1"
                     /translation="VEEHTPELTSRLHEVSGILQRLDMAGTVQLVRQLAPGVQKLWVL
                     VENSPTGKNMRLELEQLAEPQKCGLRFEFPDSESFDQLFERARSLGSDSAVLYLVLAH
                     DNQGKIMEPSEFLSRLSHVTAAPLFILADDRFVEGVVGGSLLSAYDHGKAAGQLAVRR
                     LSSPASFAAQIATSASGIVTLDYHQLERYGWDQSRVPANARILHRPPSLSEKYPLLLS
                     VTLPVTITLTTFVIVLLINIQRRRRVEAALRASQEKYRRLFTSMREGVALCEFTGTGS
                     EATDYLVLEANPAFLALFGLSADALGALASRIWPREEPPFLSAFNAMRNTGHDWTGEA
                     TCDHGRRTFRLDCFHIEGQRFAAVVDDVSDRKKSERTLFETLNMLNTVISASPSPIIT
                     FDANGCVSLWNSAAERMFSRIATEVLGRPLPIVNDSERADFLAKVNAARLAKPPSFEW
                     TLRHPDGRAQNFLVSIGTLTERPGTESGRLAIFLDVSEVRTAEREQKRMEEQLQQVQK
                     LEALGRLAGGIAHDFNNQLTTIIGNSEMLRDELRGNPESLSLVEEILAAGKRSGALTE
                     QLLTFSRRQSVTPQVVDANLVLRRLQSTLSRLLGETIRLHFDQMASDCFIRVESGLFE
                     QALMNLSINSRDAMEKGGTLEFRTSRLILKQPIQAYAQDVPVGDYVLVEVIDSGHGMD
                     TRVIENLFDPFFTTKPIGKGTGLGLAMVYGFMQQHHGYIQVHSKPNTGTRFSLYFRAE
                     PRPSESSRIEKQITPNTAAPTYTILVVEDELPVQRMMQRILKMAGYEVLTAANGTEAL
                     SVFESVSQRVDLVLSDIVMPEMGGLELATRLWEKRSDLKVILSSGYGEQALEQAPFPR
                     EHVRVISKPFPTDELLGLIRTFLDKHPV"
     gene            2664..5549
                     /locus_tag="PKO06_03070"
     CDS             2664..5549
                     /locus_tag="PKO06_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68654.1"
                     /translation="MIPAPLLSRVVRWLGFTVCFLAFPLMLVNVGLQHGLDSQWQAYL
                     ERCAARMVRQHDLLTAPAADRSFFYRDLERLVKALERNATTTTAFTDRLSLLQKKLPG
                     LFDLYLTDGSGELLYPRDLPPVLSRFALKKLHETLGRALLVGAPKPLRKHQKLFTYFW
                     GETIPWVDLSALHDVGELLEVSPVGRRSFFFYSFRQRFALFAHVNLQAVPPHFPLRVL
                     TSRASQASPDQTYGVAGVGEHLQNSRLSEAERQFVERGLVEFETSPREYLVSDDLLLV
                     VTALDPSFRLWSLSRLPPEKRGDLLRRRTIAYSVLVFLVTTVLSGMIIVGQYPVQISI
                     QSRLTGILGFAIGLPLVVILVMGYDYLWKSEQTLIHQAFRNLEESLRSLDEKFPQLFR
                     HVHQRIKNVVKRHRASDGVLRLEPFNQELIRRQAELGFYWHLIVNADGDTLYVSLDQK
                     GQRRDDTVSYSVTSEMLRRLNRSLGFVDSQKIAGRGSTSVFLSEDRSFKLFMKQSMKR
                     FNQFNRYFDRIFLFEPVWAADGRAEMMICFNFKPRDLFFLYLKQDLLTRQRQVADSQI
                     LAVNPEFPDFDTPAGAGKADWIKQFIRRVQTRKALLKERLWVDGREILAAAAPSQSGD
                     GYYLVQTVPVATVINSLTVIRWNLGLFIGISLLMVMAVGSVLARHFLLPIKDLGEGVL
                     AIQESRYRHRIPTHSRDELGQLSLMFNQTLERLEELSLARTVQGRLFPQQMLEVGEWR
                     VYGRCVTATELGGDYYDYFAVGDEYLVVVIGDVAGHGTSAALLMAMAKAGLTLEVKRD
                     VSPVQVLGALNSLLHSSDKRRKLLTFFYAVIQVASGEVRYGNAGHNRPLFFRRGVPPV
                     ELKSHGLPLGSRKNVEFRQEAFTMAAGDRLWLYTDGIPETPGHDQEPLGYERMAQVFA
                     ETASRSFDTAVHTVFQHCDAFRAGAPQADDMTLILVERTSTPTTHSG"
     gene            complement(5541..>6097)
                     /locus_tag="PKO06_03075"
     CDS             complement(5541..>6097)
                     /locus_tag="PKO06_03075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV68655.1"
                     /translation="KHTNAYFTGLGSKKRIVLYDTLIKSHTVDEAALIFAHEVGHWKH
                     DHMRIGFTLAFLGTLLGCLVAWWGFPWLQQEPALHLREIWHPVNLPFFLVVVTVSTLL
                     FAPFEAQISQHFERQADRASLELTGLKQVFIDAEVRLARDNHAELLPHPLRVFWLYSH
                     PPAIDRIRMAEEYAPTAASSPASP"
BASE COUNT         1241 a         1790 c         1734 g         1332 t
ORIGIN      
        1 gtcgaggaac ataccccgga actgacgtcc cgactgcacg aagtttccgg cattctgcaa
       61 cgtctcgaca tggccggaac ggttcagttg gtccggcaac ttgccccggg tgtgcagaaa
      121 ctctgggttc tcgtcgaaaa cagtccgacc ggaaaaaaca tgcgtctcga actggaacaa
      181 cttgcagagc cccagaagtg tggcttgagg ttcgagtttc cagactccga atcttttgac
      241 cagctgtttg agcgagctcg ctccctcggc agtgacagcg ccgtgctgta tctggttctc
      301 gcccacgaca atcagggcaa aatcatggag ccttcagagt ttctgtctcg cttgagccac
      361 gtgaccgctg cgccgctttt cattctcgct gatgaccgct tcgtcgaagg cgttgtcggc
      421 ggatctttgc tcagcgccta cgaccatgga aaggcggcgg ggcaactggc ggtccgtcgt
      481 ttgtccagtc ctgcgtcgtt tgccgctcag atcgcgacct ctgcttcggg aatcgtcacg
      541 ctcgactatc accaactcga gcgatacggc tgggaccaat cccgcgttcc agccaacgcc
      601 cgtatactgc atcgcccacc cagtctgtct gaaaagtacc cgttgctgtt gtcggtcaca
      661 cttcccgtga ccatcaccct gacgacattt gtcatcgtgc tgctgatcaa tattcagcgc
      721 cgtcgtcgag ttgaagcggc attgagagcc agtcaggaaa agtatcgacg cttgttcacc
      781 tcgatgcgcg agggagtcgc tctctgcgag ttcacgggca ccggctcaga ggcgaccgac
      841 tacctcgtgc tcgaagcgaa tcccgcattt ctggcactgt tcggactttc tgccgatgcg
      901 ttgggggccc ttgccagtcg catctggccg cgagaagaac caccttttct ttcggccttc
      961 aacgcgatgc gcaatacagg acacgactgg acaggagaag cgacctgcga ccacggaagg
     1021 cgaacgtttc gcctcgattg tttccatatc gaaggtcagc gctttgccgc cgtcgtggac
     1081 gatgtctcag atcggaagaa gtcggaacga accctgtttg agacgctgaa catgctgaac
     1141 acggttatct cggcatcgcc ttcgcccatc atcacctttg acgccaacgg atgcgtcagc
     1201 ctgtggaaca gtgccgcaga gcggatgttc agccgtatcg ccaccgaagt tctcgggcgc
     1261 cccctgccga tcgtcaacga cagtgagcgc gccgatttcc tcgccaaggt gaacgcagct
     1321 cgtctggcga aacctccatc ttttgaatgg acacttcggc atccggacgg ccgggcccag
     1381 aactttctgg tctcaatcgg gacactcacg gaaagaccgg gtaccgagag cggccgcctg
     1441 gctatctttc tggatgtgtc ggaagtacgc accgcagaac gcgaacagaa gcggatggaa
     1501 gagcaactac aacaggtaca gaagctcgag gcactcggcc gacttgccgg cggtatcgct
     1561 cacgatttca acaaccagtt gacaaccatc atcggcaact cggagatgct cagagacgag
     1621 ctgcgaggaa acccggaaag tctgagtctg gtcgaggaga tcctcgccgc gggcaaacgt
     1681 tcaggggccc tgaccgagca actgctcacc ttcagtcgac gccagtcggt aaccccgcag
     1741 gtggtcgacg ccaacctcgt gttgcgtcgc ctgcagtcga cattgtcccg tctgctcggc
     1801 gaaacgatca gattgcattt cgatcagatg gcctccgatt gcttcattcg ggtcgagtca
     1861 ggattgttcg aacaagccct gatgaacctg tcgatcaact cccgcgacgc catggagaag
     1921 ggtggcaccc tcgaatttcg taccagtcgc ctgattttga aacagccgat acaggcgtat
     1981 gcgcaggatg ttcccgtggg tgattacgtt ctggtcgaag tcatcgacag cggacacggc
     2041 atggacacca gagtcattga gaatctgttc gacccattct ttacgaccaa gcccatcggc
     2101 aagggaacgg ggctgggtct ggccatggtg tatgggttca tgcaacagca tcacgggtat
     2161 atccaggtac acagcaaacc caacaccggg acccgttttt ccctgtactt tcgcgctgaa
     2221 ccgcgtccgt ccgagtcttc acgcatcgag aagcagatca caccgaatac tgcagcacct
     2281 acctacacca ttctggtcgt cgaagacgaa ctcccggttc aacggatgat gcagcgcatt
     2341 ctcaaaatgg cgggatacga ggtgttgacc gctgccaacg ggacagaggc tctgtccgtt
     2401 tttgagtccg tcagccagcg tgtcgatctg gtcctttccg acattgtaat gccggaaatg
     2461 gggggactcg agctggccac cagactgtgg gaaaaacgct cggatctgaa agtgattctc
     2521 tcgagcgggt atggagagca ggccctcgaa caagccccgt tcccgcgtga gcatgttcgg
     2581 gtcatcagca aacctttccc caccgatgaa ctgctgggtc tgatcagaac ttttctcgat
     2641 aaacacccgg tctgagccat tcgatgattc ctgcaccgct cctgagccgc gtggttcgct
     2701 ggctgggttt cacggtgtgt tttctcgcct ttccgttgat gctggtgaat gtcggtcttc
     2761 aacacgggtt ggatagccag tggcaggctt atctcgaacg ttgcgccgcg cgaatggtgc
     2821 gccagcacga tctcctgacc gctccggccg cggaccgttc atttttctat cgcgacctcg
     2881 agcgtctggt caaggccctt gagcgaaacg caaccaccac gacagcattc acagatcgtc
     2941 tttcactgct tcagaagaag ttgcccggcc ttttcgactt gtacttaacc gacggctccg
     3001 gagaactcct gtatccgcgt gacctgccac cggtcctgtc gcgtttcgcc ctcaagaagc
     3061 tgcacgagac gcttggccgc gcattgctcg tcggagctcc caaaccgcta cgcaagcatc
     3121 agaagctgtt tacctacttc tggggcgaaa cgattccatg ggtggatctg tcggctctgc
     3181 atgacgtcgg cgaactgctc gaggtctcgc ccgtcgggcg tcgctccttc ttcttctact
     3241 cgttccggca gcggttcgcg ctctttgccc atgtcaacct tcaggcagtg cctcctcact
     3301 ttcccttgcg agtgctgacc tcgcgtgcca gtcaagcctc tccggaccag acgtacggag
     3361 tcgcgggcgt tggcgagcat ctgcagaatt ccaggctgag cgaagccgaa cggcagttcg
     3421 tcgagcgagg tttggttgag ttcgaaacct cacctcgcga gtatctggtc agcgacgatc
     3481 ttctcctggt cgtgaccgcc ctcgacccct cgttccgcct ctggtcactg tcccgtcttc
     3541 ctccagaaaa gcgcggtgat ctgctgcggc gacgaaccat cgcctattcc gtgctggtgt
     3601 tcctggtcac aactgtgctc agcggcatga tcatcgttgg acagtacccc gtgcagatct
     3661 ctattcagag ccgtctgaca ggaatactcg gcttcgcgat cggcctgccg ctcgtggtga
     3721 ttctggtcat ggggtatgac tacctctgga agtccgaaca aaccttgatt catcaagcgt
     3781 ttcgcaacct cgaggagtcg ttgcgcagcc tcgatgagaa gtttccgcag ctgtttcgcc
     3841 acgtgcatca gcgcatcaag aacgtcgtca agcgtcatcg ggccagcgac ggagttctga
     3901 ggctggaacc cttcaaccag gaactgattc gacgtcaggc agaactggga ttctactggc
     3961 acctgatcgt caacgccgac ggcgatacgc tgtatgtttc actcgatcag aagggacagc
     4021 ggcgcgacga caccgtttcc tattcggtga cttccgaaat gttacgccgg ctcaaccgca
     4081 gtctgggatt cgttgacagc cagaaaatcg ccgggcgtgg ctcgaccagc gtttttctgt
     4141 cagaagaccg cagtttcaag ctcttcatga aacagagcat gaagcggttc aatcagttca
     4201 atcggtactt cgatcgcatc ttcctgtttg aacccgtgtg ggccgcggac gggcgcgccg
     4261 agatgatgat ctgtttcaat tttaaacctc gcgatctgtt ctttctgtat ctgaaacaag
     4321 accttctaac gcggcagcga caggtcgccg acagccagat cctggccgtg aacccggagt
     4381 ttccggattt tgacacgccg gccggcgcgg gaaaggcaga ctggatcaag cagttcattc
     4441 gccgtgtgca gacccgcaaa gccctgttga aagaacgtct gtgggtcgat ggccgtgaga
     4501 ttctcgccgc ggcggcaccc agtcagtcgg gcgatggcta ctacctggtg cagaccgtgc
     4561 cggtagccac agtgatcaac agcctgaccg tgattcgctg gaatcttggc ctgttcatcg
     4621 gtatcagcct gctgatggtg atggccgtcg gttcagtgct cgcgcgacac tttctgctgc
     4681 ccatcaagga tcttggtgag ggcgtgctcg ccattcaaga gtctcgctat cggcatcgga
     4741 ttccgactca cagtcgtgat gaactcggac agttatctct gatgttcaat cagacgctcg
     4801 aacgcctcga agagttgtcg ctggcccgca cggtacaggg gcggctcttc ccgcaacaaa
     4861 tgctcgaagt cggtgaatgg agggtgtacg gtcgttgcgt gacggcaacg gaactgggag
     4921 gcgattacta cgattacttt gccgtcggcg acgaatacct cgtggttgtt atcggcgatg
     4981 tggccgggca cggcacgtcg gccgctcttc tgatggctat ggccaaagcc gggttgacgc
     5041 ttgaggtcaa gagggatgtt tctcctgttc aggtgcttgg ggcattaaac tcgctgcttc
     5101 acagctcgga caaacgcaga aagcttctca cgttttttta tgccgtgatt caggtcgcct
     5161 cgggtgaagt gcgctatgga aacgccggtc acaatcgtcc gctcttcttt cgcagaggcg
     5221 tgccaccagt tgaactcaag agtcacggtc tgccgcttgg cagccgcaag aacgtcgagt
     5281 ttcgacagga agcgttcacc atggccgcgg gcgaccgcct ctggctctac accgacggga
     5341 ttcctgaaac tccggggcac gatcaggaac cgctgggata cgagcgcatg gctcaggtgt
     5401 ttgccgagac cgcctcacgg tcgttcgata cggcggtgca tacggtgttt cagcactgtg
     5461 atgcctttcg ggcgggggcg ccccaggcgg acgacatgac gctgattctc gttgaacgca
     5521 cgtcgacgcc cacgacgcac tcagggtgac gcgggcgaac tcgcagctgt cggtgcgtat
     5581 tcttccgcca tgcgaattcg atcgatggcc ggtggatgac tgtacagcca gaaaacccgc
     5641 aggggatgcg gcagaagctc ggcatgattg tcgcgcgcca gccgcacttc agcgtcgatg
     5701 aacacctgct tcaacccggt caattcgagt gaagcgcgat cggcctggcg ttcgaagtgc
     5761 tgactgatct gggcctcgaa gggggcaaac agcagggtcg acacggtgac gacgacgaga
     5821 aagaatggaa gattcacggg gtgccagatc tcacgcagat ggagagcggg ttcctgctgc
     5881 aaccagggga acccccacca ggcgaccaga caccccagca aggtacccag aaaagccaga
     5941 gtaaaaccta tccgcatatg atcatgcttc cagtgaccga cctcgtgcgc aaagatcagc
     6001 gcggcttcat cgaccgtgtg actcttgatc agggtatcgt agaggacgat gcgtttcttg
     6061 gaccccagtc cggtgaaata ggcattcgtg tgcttcg
//
LOCUS       DAORHD010000090         6073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13103_length_6073_cov_4.078265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000090 DAORHD010000000
VERSION     DAORHD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13103_length_6073_cov_4.078265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PKO06_03080"
     CDS             complement(<1..1288)
                     /locus_tag="PKO06_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68656.1"
                     /translation="MSLLGTRPSSARLRGTISLVMLGLLSLLIIVAFGLFRRVGSHTH
                     TVTLLDQSLIVRNVLESLSADAFQFIRREANRQGSTLYQTFRGSTPTAEHVLDLAAAG
                     YAPTSAFHDLIQPYQSGSGLILTVDSQPRVSLTDVQAIPLPDLLYFADVSHTVSEYAG
                     SIKIVSSGGFGDRRYTLTARYPFKVTFVQLPMLREFAWFFDQVSTEQTATTDGINVLP
                     LKNDEIEPGHWPLILDSPFADRAATPEGGSKTTMEFGTPHQNGKVYLGNSGQPVILQL
                     GGEVQPSRAVFSDLSLIKPSHFNIITEKTDKVYLEDLSVVGFSHQVRGFSKFLGPPIS
                     PSSIRTTSWIFGFSDETTPSSGMFTRSIKFDKFLTGDPAFTTLNANPGLWRLSSALKP
                     LGLSKAPLLYAQALEQGIPPSEAIGYSIIPARQIFGQ"
     gene            1407..1751
                     /locus_tag="PKO06_03085"
     CDS             1407..1751
                     /locus_tag="PKO06_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV68657.1"
                     /translation="MATFSSPAEMVGSVFVIRPTGYLDEAGGKTLRAAFQEPLQKGCQ
                     KFLLNLTGTPVINSQGITQILEICEAVLYEQKGTLGLVGLSELYTDVFEVVGILNLVT
                     LFADEAEALKLL"
     gene            1789..2895
                     /locus_tag="PKO06_03090"
     CDS             1789..2895
                     /locus_tag="PKO06_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68658.1"
                     /translation="MATRLMRFWICAALLLSVLVGSPVAGQGLDEYESAPVADTNTVT
                     ASEPVYTGAASESAATVAVATGVTEVDDVRRRIVSFAEAEVGKVCSDAGPDGFKIGWQ
                     NLVGFYEKAFGYTNLEKDQPYWYKCLRAPNQTIKGGPWSWCGIFALSNWVKAGLPVRW
                     NTRVVGCKYRGDVKKYLQPGDIAIIKLAVNPNNHHCIVKEVNGNELVTIDGMQEKQQV
                     RQRTRKVSDIEIYYSVADAMNASMTLPNPPAPGGGSTPATPTTPSTPGSASTVSGGQT
                     PGITGSTPAVIDTKKGQELEQILQQLTEWVLREAQRVFSWARQQETPAAGGAAAGVGS
                     SGNESETPSVETPQKSFWDYVVEGVRSILPFSLF"
     gene            complement(2927..4744)
                     /locus_tag="PKO06_03095"
     CDS             complement(2927..4744)
                     /locus_tag="PKO06_03095"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68659.1"
                     /translation="MNLPNPAAPYVRKIVVLVLVPFLAFLFYAGFGIRQTWRSTEFAE
                     RVLQQADFLDQTFRLITQLQRERSFSALYLTGSAPRSQLEQEYLQTQPVLELFLASIT
                     NASLSQPLQQQSQQLRQRVSDARNAARARETFPFAYQQYNRLIDVLQDVQESMAQAFQ
                     DAETARLFNNILLIEAAREHAGKLRVNFTRLLSAPPEAEDEAMYLLFVTHTAIRELLN
                     VRALSLTRQGQQMLSAKRDSDAWKYVNDLIGHLFRGKIELSALNADPTRFVEAITRVI
                     HDLEEITANENQQVRRIQLEILERSRKQRLQTIILILFVCLFLAYLGYDYITGLMERN
                     DAYTRLQNTLAELSEARKQEISIGWQIQQDLLLGKVPTEIRGGRMACRTIPSLGIDGD
                     FIDFFAHDTNTVDILFGDVMGKGIPAALVGAATKSHFQRAMSRLLLELQQHAWPAPEE
                     IVAHVQQRMAPGLFSVERFVSLHYGQFNFVQRLLKLVNCGHTEVLHFHRANGTHSLYE
                     PQNAPLGIFETETFTSQAISFATGDVFVFYSDGITETRNAVGDFFGKERLVHSVGSIV
                     HDASFSPEIAVAKILSELLAFAGSDQFRDDLTLLVIEVA"
     gene            complement(4768..5295)
                     /locus_tag="PKO06_03100"
     CDS             complement(4768..5295)
                     /locus_tag="PKO06_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68660.1"
                     /translation="MKLVFSMLIGLAFWFFPDVTTRLFYAYAIQSVSRPHESDAALIA
                     RFQQQRRSFETLRAMIASDTGLERVDDTWTSPKDPATIGITPQRIAEYRRLFNHIGTP
                     RGIEAYDPTRSSFRLLASTQGITVSGSGKGYLWSTEPPSPLLSELDSALASHSKPFFG
                     YRHLEDRWYLFLEID"
     gene            complement(5292..>6073)
                     /locus_tag="PKO06_03105"
     CDS             complement(5292..>6073)
                     /locus_tag="PKO06_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNV68661.1"
                     /translation="IFSGGTMHLQGVKTGLIVLLITLFSCVAFGLAAEPASPSESLQW
                     SPGEFKKLKSTLEFENEALSQPDMKLQDVRISPLIRYERWYEIPIEIGFHTAEDGIVS
                     FMKKAHAYSLANATLGHKAITISQTAETLPNGKQLLSVIVSQALVVGQAPLNRHSENL
                     VKGLDQILRQTTFEPRVRRGGGEVAKSAPHWITNLRIDSDLRFQITGYGLSLSAVTEL
                     AEKLFDSTIFIDVNLNSATRVVFEKQPIWRFDLVARVSAQE"
BASE COUNT         1355 a         1564 c         1870 g         1284 t
ORIGIN      
        1 cctgcccgaa gatctggcgt gccggaatga tcgagtagcc gatggcttcg gaaggaggaa
       61 tgccctgctc gagagcttga gcgtacagaa gcggagcctt cgaaagtccg agtggtttga
      121 gcgcggaaga cagtcgccag agaccgggat tcgcattgag ggtggtaaag gccgggtcac
      181 cggtcagaaa cttgtcgaac ttgatcgacc gggtaaacat cccgctcgat ggcgtggttt
      241 cgtcggagaa accgaagatc cagctggtgg tacgaatact cgaagggctg atgggtggcc
      301 cgagaaactt gctgaacccg cgcacctggt gtgagaagcc gacgacgctg agatcctcga
      361 ggtagacctt gtcggttttc tcggtgatga tgttgaagtg agaaggcttg atcaggctca
      421 gatcagaaaa gacggcgcga ctgggttgta cttccccgcc cagttgcaga atcactggct
      481 gaccgctgtt gccgagatac actttcccgt tctgatgggg agtgccgaac tccatcgtgg
      541 ttttcgagcc gccttcggga gtggcggcac ggtcggcaaa cggcgaatcg agaatcagtg
      601 gccagtggcc cggttcgatc tcatcgtttt tcagtggcag tacgttgatt ccatcggtcg
      661 tcgcggtctg ctcggtggac acctggtcga agaaccaggc aaactcgcgc aacatcggca
      721 actgtacgaa cgtgaccttg aagggatacc gagcggtcag agtgtagcga cgatcgccga
      781 agccgcctga gctgacgatt ttgatcgagc cggcatactc gctgaccgtg tgcgagacat
      841 cagcgaagta caagagatca ggcaacggaa ttgcctgaac atcggtaaga gaaacccgcg
      901 gttgagaatc gaccgtcagg atgaggccac tgccgctctg gtaaggctga atgaggtcgt
      961 gaaatgcgga tgtaggagcg taacccgccg cagcgagatc gagcacgtgt tcggcggtcg
     1021 gcgttgagcc gcgaaaggtt tgatagagag tggaaccctg acgattggct tcgcggcgga
     1081 tgaactgaaa agcatcggcg gaaagcgact cgaggacgtt gcggacgatg agcgactggt
     1141 cgagcagcgt gaccgtatgc gtgtgagaac cgacacgtcg gaacaacccg aaggcaacga
     1201 tgatcaacag gctcagcaag ccgagcatga ccagtgaaat ggtaccccgc aggcgcgcgg
     1261 aagacggtcg ggtgcccaga agactcatga ataccatggc gccagtatac tacgctcgct
     1321 ttgaagagtg agcaggcagg cgaggtggct cgtgcggtcg ttcggcaatg attgactttc
     1381 acggggaccg gtgcttaatt gaagacatgg cgacgttttc atcccctgcg gaaatggtcg
     1441 ggtctgtctt tgtcattcgc cccacggggt atctggacga agcaggcggc aagactctgc
     1501 gtgcagcatt tcaggaaccg ttgcagaagg ggtgccagaa gtttctgctc aacctcaccg
     1561 gaaccccggt gatcaacagc cagggtatta cccagattct cgaaatctgc gaagccgttc
     1621 tgtacgagca gaaaggaact ctcggcctgg tcggattgtc ggagttgtat accgacgttt
     1681 tcgaggtggt tggcatcctg aatctggtca cgctcttcgc ggatgaagcc gaagccctga
     1741 aactgctgta aagacgggtt tcggatcggt ctgaaacaag gaggctccat ggcgactcga
     1801 ctcatgcgtt tctggatctg tgccgctctg ctgttgtccg tgctcgttgg ttcaccggtt
     1861 gccggtcagg ggctcgatga gtatgagtcg gcgccggtgg ccgatacaaa cacggtgacc
     1921 gcctcagagc ctgtctacac gggcgcagct tcggaaagcg ccgcgaccgt cgctgtggcg
     1981 accggggtta cagaggttga cgacgtgcgc cgtcgaatcg tcagcttcgc tgaggctgaa
     2041 gtcggaaaag tgtgcagcga tgctggccct gatggtttca agatcggctg gcagaatctc
     2101 gttggttttt acgagaaggc cttcggctat acgaatctcg agaaagatca gccatattgg
     2161 tacaagtgtc tgagagcgcc gaaccagacg attaaaggcg ggccgtggtc ctggtgcggt
     2221 atctttgccc tttccaactg ggtgaaagcc ggtttgccgg tgcgctggaa cacgcgcgtt
     2281 gttggctgta aataccgggg tgacgtgaaa aagtatctgc agcctggcga tattgccatc
     2341 atcaaattgg ctgtcaatcc caataatcat cactgcatcg tgaaggaagt caatggcaat
     2401 gaactggtca ccattgacgg catgcaggaa aagcagcagg ttcgccagcg cacccgcaaa
     2461 gtcagtgata tcgagatcta ttactcggtg gccgatgcca tgaatgcgtc gatgactctg
     2521 ccgaatccgc ccgcgccagg cggcggatcg acacctgcca ccccgacgac accttcgacg
     2581 ccggggtccg cttcgactgt cagtggcgga caaacacccg ggatcacggg ctcaaccccg
     2641 gccgtcatcg atacaaaaaa gggacaggaa ctggagcaga ttctgcaaca actgaccgag
     2701 tgggtgctgc gtgaagccca gcgcgtgttt tcgtgggccc gacaacagga aacaccggca
     2761 gccggcggtg ctgcggccgg tgttggttct tcgggcaacg aatcagagac tccgtccgtc
     2821 gaaacaccgc agaagagctt ctgggactac gtggtcgaag gtgtgcgttc gattctgcct
     2881 ttttctcttt tttgaagcat ctcttcgatt ctcatcgctc gtactctcag gcaacctcga
     2941 tgacgagaag cgtcaggtcg tcgcggaact gatctgaacc ggcgaaggcg agaagttcag
     3001 acagaatttt tgccacggcg atctcggggc tgaatgaggc atcgtggacg atggatccga
     3061 cgctgtgaac cagtcgctct ttgccgaaga aatcgccaac ggcgtttcgc gtttcggtga
     3121 ttccgtctga gtaaaagaca aagacatcgc ccgtggcgaa cgaaatcgcc tgtgacgtga
     3181 acgtttcggt ctcaaagatg ccgagcgggg cattctgcgg ctcatacagc gagtgcgttc
     3241 cgttggcgcg gtggaagtgc agaacctcgg tgtgcccgca gttcaccagc ttgagaagtc
     3301 gctgaacaaa gttgaactga ccgtaatgca ggctgacgaa gcgttcgacg ctgaacaacc
     3361 cgggggccat tcgttgttgc acgtgggcga cgatttcttc aggtgccggc caggcgtgtt
     3421 gttgcagttc aagcagcagg cgcgacatgg cgcgctgaaa gtggctcttc gtcgccgcgc
     3481 cgaccagcgc tgccggaatt cccttgccca tcacgtcgcc gaacagaatg tcgacggtgt
     3541 tggtgtcgtg cgcgaagaag tcgatgaaat cgccgtcgat tcccagagag ggaatggtgc
     3601 ggcacgccat gcggccaccg cgaatctcgg ttggcacttt cccgagaagc agatcttgct
     3661 gaatctgcca cccgatactg atttcctgct tgcgcgcctc tgacaattcg gcaagagtgt
     3721 tttgcaggcg ggtgtaggca tcgtttcgct ccatcaagcc cgtgatgtaa tcatagccga
     3781 gataggcgag aaacagacag acgaacagaa tgaggatgat ggtctgaagt ctctgttttc
     3841 gagaccgttc cagaatctcg agctggatgc ggcgaacctg ctggttttcg ttggcggtaa
     3901 tctcctcgag atcgtgaatg acgcgggtga ttgcctcgac gaatctggtt gggtccgcgt
     3961 tcagagccga caattcgatc ttgccgcgaa acaggtgacc gatcaggtcg ttgacatact
     4021 tccaggcatc tgaatcgcgt ttggcggaaa gcatctgctg tccttgccgg gtcagagaca
     4081 gagctcggac attcaacaat tcgcggatgg cggtatgggt gacgaatagc agatacatgg
     4141 cttcgtcctc cgcttccgga ggggcactca gcaggcgagt gaagttcact ctcagcttgc
     4201 cggcatgctc acgcgccgcc tcgatcaaga ggatgttgtt gaagagccgt gctgtttccg
     4261 catcctgaaa ggcttgtgcc atcgactcct gtacatcctg cagaacatcg atcaggcgat
     4321 tgtactgctg atatgcgaaa ggaaaggttt cgcgagcccg cgcggcattc cgggcatcac
     4381 tgaccctctg gcgcagctgt tgtgattgtt gttgcagggg ttgtgacagc gaggcgttgg
     4441 tgatgctggc caggaataac tcgagaacag gctgagtctg cagatattcc tgctcgagtt
     4501 gggatctggg cgcgctgccg gtcagataca gggcagaaaa cgaacgttcg cgctgcaact
     4561 gagtaatcag tcgaaaggtt tgatcgagaa aatcggcctg ctgcagcacc cgctcagcga
     4621 actccgttga acgccaggtc tgccggatac cgaagccggc atagaacaga aaagcaagaa
     4681 atggcaccag aaccaggacg acgattttcc gcacataggg agcggcagga ttgggcagat
     4741 tcacgtaggc tccttgaaaa acgttcccta gtctatttcc agaaagagat accaacggtc
     4801 ttcgagatga cgatacccga agaacggctt gctatgactg gcaagagctg aatcgagttc
     4861 ggacaaaagt ggcgacggtg gttcagtcga ccataagtac cctttaccgg aacctgagac
     4921 ggttattcct tgcgttgaag cgagcaggcg aaatgaagat ctggtaggat cgtaagcttc
     4981 aatacctctg ggagtgccga tatgattgaa gagtcgccgg tactctgcaa ttcgttgagg
     5041 cgtgatgcca atcgtcgcgg gatcctttgg gctggtccag gtgtcatcga cacgctcgag
     5101 accggtgtcg cttgcgatca tggctcgcag agtttcgaag cttcgccgct gctgttgaaa
     5161 gcgggcgatc aaggcggcgt cgctttcgtg cgggcgactg acggattgaa tggcatacgc
     5221 gtagaacagt cgcgtggtca catcgggaaa gaaccagaag gcgagcccga tgagcatgct
     5281 gaaaaccagt ttcattcttg ggcgctgact ctcgcaacga gatcaaaacg ccagatgggc
     5341 tgtttctcga agacgacccg ggtggcactg ttcaggttca cgtcgatgaa aatggtgctg
     5401 tcgaagagtt tttcggcaag ctcggtgacg gccgaaagcg agagcccata accggtgatc
     5461 tgaaaccgca gatcgctgtc gatccggaga ttcgtgatcc agtgaggcgc actcttcgcc
     5521 acctccccgc ctccccggcg aacgcggggt tcgaacgtgg tctgtctgag aatctggtcg
     5581 agacccttga cgagattctc gctgtgcctg ttgagcggtg cctgtccgac caccagagcc
     5641 tgactgacga tcaccgaaag aagctgttta ccgttcggca gtgtttcggc ggtctgagag
     5701 atggtaatcg ccttgtgtcc cagggtggcg ttggccagcg aatacgcgtg ggctttcttc
     5761 atgaagctga cgattccgtc ttccgcagtg tgaaagccga tctcgatggg aatctcgtac
     5821 catcgctcat accgaatcag cggcgatatg cgcacgtcct gcaacttcat gtccggctga
     5881 ctcagagctt cattttcgaa ctccagagtc gacttgagtt tcttgaactc gcccggagac
     5941 cactgcagac tttcagaggg ggatgcaggt tcggcggcca gaccgaacgc gacacacgag
     6001 aaaagagtga tgagcagcac aattaaaccg gttttcacgc cctgaaggtg catcgtgcct
     6061 cctgaaaaga tcg
//
LOCUS       DAORHD010000091         6058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13139_length_6058_cov_3.556888, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000091 DAORHD010000000
VERSION     DAORHD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13139_length_6058_cov_3.556888"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PKO06_03110"
     CDS             complement(<1..1163)
                     /locus_tag="PKO06_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV68662.1"
                     /translation="MDIDLSQYMGMYVDGSRENLDMMDKQLLELEQDPASVSAVGEIF
                     RAAHTLKGMSATMGFEKVAHLTHEMENILDKLRNRQMTANQQVIDVLFETFDVLRILV
                     NDSISATDSNINLDAIIKKLNDLMNQTSGIEKVVEAQKVVNTAASATTAASAAPTGSG
                     TLSAMADISFNEFEYQVLLEAQQRNQSIFLMQVKLVNDCLLKAPRAFMVVRNLEELKC
                     EIIKSVPEVKDLEEEKFDLSFKMVLVSSSTGNDVKNGIESISEIERVDVTKISAEDCR
                     QAAGAQVAAAAAAPPPPAAPPPVVAAPKPSPTIAAPVATPPRPPAPARPAPPPPAPTP
                     VPAKPAAALPPAPVAKAGGAPPAPPPPPVVPPAAPPGSPPKGGGKEKAASAAE"
     gene            complement(1453..3054)
                     /gene="flhF"
                     /locus_tag="PKO06_03115"
     CDS             complement(1453..3054)
                     /gene="flhF"
                     /locus_tag="PKO06_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNV68663.1"
                     /translation="MTLKRYTADSFLEAFLQAQEELGEDMIVVTSGTVQQNKFGGIYR
                     TTVWELTAMPGKAKATPKSVGSFNAPSTPVVSEEIFLPPSEPARKASRPATQPAAGIS
                     GTSARAPFTAMNTAAESARAVAGTSASHQSSLRGIHTIRSNQIGDHEPQLQRQDSDQQ
                     NAAKLQAMLGEILKNRRDVPMAPRPEATSTRPAAPNAAGAVARNPQAAAYQNVQVAPS
                     AVRELDKEETPAIQDLESKINDIFSMLRQIKGASDKVLGTPSCSLPDGLKMLRERLVQ
                     QEAPEDFIDELVEQMREILPLQAQRNPTLVWDESCRWLQQQLQFRAPVIARQSSGPTI
                     IILMGPTGVGKTTTIAKLAASFALDVQQRRKVALFTIDTYRIGAPAQLSQYAQIIEAD
                     MEILLMPDEVNPALVKHADKDIILVDTAGRSQKNQRELQDMKQFLDCFPDSVKFLVLS
                     ATTKFSDMVENERRFGELGYDHFIFTKTDETNSFGGLLALMYRSRSPLAYLTHGQSVP
                     DDFRKAGFEFFLSQMQASSPLHQNL"
     gene            complement(3051..3992)
                     /locus_tag="PKO06_03120"
     CDS             complement(3051..3992)
                     /locus_tag="PKO06_03120"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNV68664.1"
                     /translation="MADSVAKPIDSMIAQTRERIQRYVFPRLESRKPKVPGRKIARTV
                     AVTSGKGGVGKTNLVANMAISLAQSGKRVIILDADLGLANIDVVFGIRPKRNIMDVIS
                     GSFRLEQILIPGPCGIQIIAGGSGIAELAHLDQKRVESLFEQMRFLEDRADYLLIDTG
                     AGIAPTIISFCLAADQIIVVTNPEPTALADAYGIIKIISQHRSDAYVGVLVNRAESEQ
                     EAGFIHERLVKVAGDFLQFPVHRFGFVPQDKQMYLAVRQQTPLLLFSPTSAASNAIRR
                     VVHQVFNLSSNEELPQQEGISGFFQRLNALFRGGQTE"
     gene            complement(3985..4845)
                     /locus_tag="PKO06_03125"
     CDS             complement(3985..4845)
                     /locus_tag="PKO06_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68665.1"
                     /translation="MMPDQAAALRQLKEMMDRQAAEQTPTGETFLATLPVTTPFTTIL
                     LLMPEDQSQSLPPMQKWLPRFFQNPRKACLWDEGGYLPAKLALSEQASANLLSPMQQL
                     ETPHGPIWVIPRFGRLGNLTNEPEVDRIRFLKQAHHALSSIQELWITLSNRDVHRNHP
                     IVASADLAALMLPFHPNSLLRGYEIVKTLHLAKFVNPIGILVEQAGAGTSTDDLFQRL
                     QATAKRFLSLDLQFGGVVHSQERADSDIPSTPLQTMLNSASPLPRTFLTRFADHVLFP
                     HPSLTGGTHG"
     gene            complement(4851..>6058)
                     /locus_tag="PKO06_03130"
     CDS             complement(4851..>6058)
                     /locus_tag="PKO06_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNV68666.1"
                     /translation="MAFLALIPGLPKFSFALLAAIFGSLAYVINKTSTEQQQVQEEEA
                     KAQEQAAAAPKPEDVSSLLSVDPLELEIGYNLIPLVDTNQGGDLLNRITLIRRQMAID
                     LGLIVPPIRIRDNIQLHPSTYLVKIKGVEIDRYELIPDQFLAMNPGTVTSPIAGIEVR
                     EPAFGLPAVWINQSMRERAELAGYTVVDPSTVIATHLTEIVRRNSHEILGRQELQSLL
                     DTVKKSYPLIVDEVVPSVVNQATLLKVLQNLLREGVPIRNMVTIMESLADCAGVKDVD
                     ALTEIVRQGLSRHICKTLVDENGVLNVISLEPRLEQVLTGSLQKIDGSVQLAMDPKLG
                     QNFMDRLKEKVEEVMNEGRTPILLCSSPLRLSIKRLSERIAPRLVVLAYNEIPSTLQL
                     QSIGLVGLN"
BASE COUNT         1236 a         1600 c         1881 g         1341 t
ORIGIN      
        1 ttttctgcgg cagacgctgc tttctccttg ccgcctcctt tgggaggact gcccggcgga
       61 gctgctggcg gtacgactgg cggtggtggt ggtgccggcg gggctccacc ggcttttgcg
      121 accggggccg gcggaagagc ggcggcaggt ttggcgggga cgggagtggg agctggtggc
      181 ggcggtgccg gccgtgcggg tgctggcggc cgcggcggag tggctaccgg cgcggcgatg
      241 gtcggagacg gtttgggagc ggcgacgacg ggaggcggtg ccgctggcgg cggtggggcg
      301 gcagcggctg ccgccacctg ggctcctgct gcttgtcggc aatcttcggc agagattttg
      361 gtgacgtcga cgcgttcgat ttcggagatt gattcgatcc cgttcttcac gtcgtttccg
      421 gtgctggaag acaccagcac cattttgaaa gaaaggtcga acttctcctc ttcgagatcc
      481 ttgacttcag ggaccgactt gatgatctcg cacttcagtt cctcgagatt gcgcacgacc
      541 atgaaggctc gtggcgcttt gagcagacag tcgttgacca gcttgacctg catcagaaag
      601 atggattgat tccgctgctg agcttcgaga aggacctgat actcgaactc gttgaaactg
      661 atatcggcca tagccgagag ggttcctgac cccgtcggcg cggcggacgc tgctgtggtc
      721 gccgaagcag cggtattgac cactttctgg gcttcgacaa ccttctcaat tcctgatgtc
      781 tggttcatca ggtcgttgag cttcttgatg atggcatcca ggttgatgtt tgagtcggtg
      841 gccgaaatac tgtcattcac cagaatgcgc agtacatcaa atgtttcaaa caggacatcg
      901 atgacctgct ggtttgcggt catctggcga tttcgcaatt tgtcgaggat gttttccatc
      961 tcgtgagtga gatgggcaac cttctcgaag cccatcgtgg cggacatccc cttcagcgta
     1021 tgggcggcgc gaaagatctc tccgaccgcc gaaacactgg ccggatcctg ttccagctcc
     1081 aggagctgtt tgtccatcat gtcgaggttt tcgcgagaac catcgacata cattcccatg
     1141 tattgggaaa gatctatatc cacggacatc cccgaggaaa aagttacttg tgctctggtt
     1201 ccccgcggtc ttcgcctgtg cttcgcagat ctgaagaaca cttcacgggt tcggttcgcg
     1261 gttgtgagag acctcgccgc agcccaaaac ccgacaggcg aaagaaacgg ggttctttcg
     1321 cacggcccgt atcataccat atcggcactc cgggggcgat ttccaacgca tgggaaattc
     1381 gagaatgcgg atgaggggaa accggcagta ctcgatgaaa cggcaaccag cgttttgagt
     1441 ggggaaaacc gactacagat tctggtgaag tggtgacgac gcctgcatct gcgacagaaa
     1501 aaattcgaaa ccggccttcc ggaagtcgtc gggaacgctc tggccatgag tgagataggc
     1561 cagcggggag cggctgcgat acatcaaagc cagcaggccg ccaaaggagt tggtttcatc
     1621 ggtcttggta aagatgaaat gatcataccc aagttcgcca aagcgccgct cattttcgac
     1681 catgtcagag aacttggttg tggctgacag caccaggaac ttaactgagt ccgggaaaca
     1741 gtcgagaaac tgtttcatgt cctgcagttc acgctgattc ttctgcgatc ggcccgcggt
     1801 atcgacaaga atgatgtcct tgtcggcgtg cttgaccaag gccgggttga cctcatcggg
     1861 catcaacaga atctccatgt cggcctcgat gatctgcgca tattgtgaaa gctgtgcggg
     1921 tgcgccgata cgataggtgt cgatggtgaa cagggcgacc tttcttcgct gctgaacatc
     1981 gagggcgaat gaggcggcca gcttggcaat cgtggtagtt ttgccgactc cggtcggacc
     2041 catcaaaatg atgatcgtgg gcccgctgct ctgccgtgca atgacgggag cccggaactg
     2101 aagctgctgt tgaagccagc ggcaggattc atcccagacg agagtcgggt tgcgctgggc
     2161 ctgcagtggc agaatctctc gcatctgttc gacgagttcg tcgatgaaat cttcgggggc
     2221 ttcctgctgt accaggcgtt cgcgaagcat cttgaggccg tcgggaagac tgcaggaagg
     2281 agtgccgaga accttatctg atgccccctt gatctgtcgc agcatcgaaa aaatgtcgtt
     2341 gatcttgctc tcgagatcct gaattgccgg tgtctcttct ttgtctagtt cgcgaactgc
     2401 gctcggagca acttgtacgt tttgatatgc cgccgcctga ggattccgag ccaccgctcc
     2461 tgcggcgttg ggcgcggcgg ggcgcgtgga tgttgcttcc ggtcttggtg ccatgggaac
     2521 atcgcgacgg ttcttcagga tctctcccag catggcctgc agcttggccg cgttctgctg
     2581 gtcgctgtcc tgtcgctgca attgcggttc gtggtcgccg atctggttgg agcgaatggt
     2641 gtgaatgcca cggagagaac tctgatgtga agccgatgtg ccagccacgg cgcgagcgga
     2701 ctcggctgcc gtgttcatgg ccgtaaacgg agcccgcgcg gacgttccgc tgatgcctgc
     2761 tgctggctgg gttgcaggtc gagaggcttt tcgtgcgggt tccgagggtg gcagaaaaat
     2821 ctcttcggaa acgaccggtg tgcttggcgc gttgaaactg ccgaccgact tgggggtggc
     2881 ttttgccttg ccgggcatgg ccgtgagctc ccagaccgtg gtacgataga tgccaccaaa
     2941 cttgttttgt tgcaccgtgc ctgacgtgac gacaatcatg tcctcgccaa gctcttcctg
     3001 ggcttgtaga aaggcttcca gaaaactgtc ggcggtatag cgtttgagtg tcactcggtt
     3061 tgccctccgc gaaacagggc gttcaggcgc tggaagaagc cggagatgcc ttcctgttgc
     3121 ggcagttctt cattcgacga gagattgaat acctgatgca cgacacgccg aatggcattg
     3181 gaagcggcag acgtcggtga gaagagcagg agcggggtct gctggcgcac cgcgagatac
     3241 atctgcttat cctgcggcac gaaaccgaat cgatgcaccg gaaactgcag aaagtcaccg
     3301 gcaaccttga cgagccgttc atgaatgaag ccggcttcct gctcggactc ggcacggttg
     3361 accaggacac cgacgtaggc gtcgcttcgg tgctggctga tgattttgat gatgccgtag
     3421 gcatcggcca acgcagtggg ttcgggattg gtcacgacaa tgatttgatc agcggcgaga
     3481 cagaacgaga tgatggtggg ggcaatgccc gcgccggtat cgatgagcag atagtcggct
     3541 cggtcttcaa ggaagcgcat ctgttcgaac agactctcga cgcgcttctg gtcgagatga
     3601 gccagttcgg cgatacccga accacccgcg atgatctgaa tgccgcatgg gcccggaatc
     3661 aggatttgtt ccagtcgaaa cgagccggaa atgacatcca tgatgttgcg ctttgggcga
     3721 atgccgaaga ccacgtcgat gttggccaga ccaagatcgg catcgaggat aatcaccctt
     3781 tttccggatt gggcgagact gatcgccatg ttggcgacca ggttggtctt gccaacccca
     3841 cccttacctg atgtgactgc aaccgtgcga gcaattttgc gacctgggac ttttggcttg
     3901 cgcgactcga gtcgcggaaa cacataccgc tgaatacgct cgcgggtctg ggcgatcatg
     3961 ctgtcgatcg gcttggcgac ggaatcagcc atgagtgcct ccggtcaggg acgggtgagg
     4021 aaaaagaaca tgatcggcga aacgcgtcag gaaggtgcgc ggaagtggtg aagcactatt
     4081 gagcatggtt tgcaacgggg tggagggaat gtcggagtcg gcacgttcct gagaatgtac
     4141 caccccgccg aactgcaggt caagagagag aaagcgtttt gccgttgcct gaagtcgctg
     4201 aaacaggtcg tcggtcgaag tgcccgcgcc cgcctgctcg accagaatac caatgggatt
     4261 cacaaatttt gccaggtgca gagttttgac gatctcatac ccacgcagca gcgaattcgg
     4321 gtgaaaggga agcatgaggg cggccagatc cgcgctggcg acgatggggt gattgcgatg
     4381 cacgtcgcga tttgagagcg tgatccagag ttcctgaatc gaactgagtg catggtgcgc
     4441 ctgcttcagg aaccggatgc ggtctacctc aggctcattc gtcagattgc cgagtcggcc
     4501 gaaccgggga atcacccaga tgggcccgtg cggggtttca agttgctgca tgggagacag
     4561 gagattcgca ctggcttgct cactcagggc cagttttgcc ggaaggtagc cgccttcgtc
     4621 ccacagacag gctttgcggg gattttgaaa gaaacgcggc aaccacttct gcatcggagg
     4681 caggctctga ctctgatctt cgggcatgag cagcaggatg gtggtaaacg gggtggtcac
     4741 cggcagagtc gcgagaaaag tttcaccagt cggtgtctgt tccgcggcct ggcggtccat
     4801 catctctttc aattgtcgca gggcggctgc ttgatcaggc atcattcacc tcagttcaga
     4861 cccacgagac cgatcgactg caactgcaga gtcgagggaa tctcattgta tgccaggacc
     4921 accaggcgtg gggcaatgcg ctcagacaaa cgtttgatgc tgagccgcag cggagaactg
     4981 cacaggagaa tcggagtccg cccttcgttc atgacctcct ccactttttc cttcagccgg
     5041 tccatgaagt tctgaccaag ctttggatcc atggcgagtt gtacggaacc gtcgattttc
     5101 tgcaggctgc cggtcagaac ctgctcgaga cggggctcaa gagagatgac gttcaaaacc
     5161 ccgttttcat cgaccagcgt cttgcagata tgtcgggaaa gaccctggcg cacgatctcg
     5221 gtcagggcat ccacatcttt gactccggca cagtctgcca gcgattccat gatcgtcacc
     5281 atgttgcgga tcggcactcc ctcgcgcaac agattctgca gaactttcag taacgttgcc
     5341 tggttgacaa cgctcggaac cacttcgtca acgatcagtg ggtaggactt cttcacggtg
     5401 tcgagcagcg actgcagctc ctgacggccg agaatctcat gagagttgcg gcgtacgatc
     5461 tccgttaagt gggtggcaat gaccgtcgac gggtcgacca cggtgtagcc tgcaagctca
     5521 gcgcgttcgc gcatgctctg gttgatccag acggcgggca ggccgaatgc cggttctcgc
     5581 acttcgatgc cggcaatggg cgaggtgaca gtgcctgggt tcatggccag aaactggtcg
     5641 ggaatcagtt catagcgatc gatttcgaca cccttgattt tgaccagata ggttgacgga
     5701 tgcagttgaa tattgtcgcg aatgcgaatc ggaggcacga tcaacccaag atcgatcgcc
     5761 atctgccgac ggatcagtgt gatgcggttc agcagatctc ccccctgatt tgtgtcaacg
     5821 agcggaatga ggttgtagcc gatctcgagt tcgagcggat caacactcag cagcgacgaa
     5881 acatcctcgg gctttggtgc tgctgcagcc tgttcctggg ctttggcctc ctcctcctgg
     5941 acttgctgct gttcggttga cgtcttgttg atgacgtaag ccagcgatcc gaaaatggcg
     6001 gccagaaggg caaagctgaa cttcggcaga ccgggaatca gagccagaaa cgccatcc
//
LOCUS       DAORHD010000092         6034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13232_length_6034_cov_3.290684, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000092 DAORHD010000000
VERSION     DAORHD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13232_length_6034_cov_3.290684"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1525
                     /locus_tag="PKO06_03135"
     CDS             134..1525
                     /locus_tag="PKO06_03135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV68667.1"
                     /translation="MSFPLVYQRPEFGKLWLAQLIAFIGDGIFQIAMVMWLIQKTNSG
                     TLVGLIISVSFLPAVLIGPFAGTLADRLPPKVLLIGADVSRGLLLAIFAGVAGNDLLE
                     VWHLFLICSLLATAGVFHSPTTLAVIPRLVSPDQIDGAMALHTVVRDLSKLIGPALGG
                     FIVARWSVPVAFACNAFALFFSAACIAMMSIPPRTATGGDKQVLTQLWEGLAYVRRQD
                     TIFRMLSGFAFLNLFAAQILVLLPLTVMQLLLRDSVALGFCEGALALGSVAIGFFFVQ
                     ILTRIPTSKLFLGSMAGSGVLFLVFAGNVRFWKTLASLFAPAATAEAMVGAPGWGSWG
                     LLVAPYPTFIVGLFLLGGCFAAVNIAALTIFQKIVAPDMKGRFFSLVEALSYVSLPLA
                     SALAGYLADHAGIPVTYVIGGVGLLILTWRFSRIPGLTSFDQAEAAAGAPSPSPDETN
                     QPAQNREPENVLP"
     gene            1522..2772
                     /locus_tag="PKO06_03140"
     CDS             1522..2772
                     /locus_tag="PKO06_03140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68668.1"
                     /translation="MSRPVRQVHLSLLAGLLPAVFLILALLAYNALIERMQSGLHEQR
                     SLALRTLPAATIAQQKHYSCVALTADLATFTILTGDWPLALDNTEIRRLREMLGSGTE
                     LGIEIELADLGPAFFWLVPIEGGYRAAVQSRLSFRSRVESFRALIWIGALLLAGIAFF
                     VYALLARKLSDVFSEMEAKNLELERANRHLEELGKLKSNFLALVSHELRTPLARMAGH
                     IQLIKAQEEQLPPEQRRRFEEMSLDVEELSRMTKNVLDLTRLQSEDLSARLDLSQVSP
                     LVRSCVERSRRGAAARGLTLECETPETPPVSHDPYLLERILDNLLVNAIKYSFPDTVI
                     RVTLVERESNVEICVESRGAVIPSADREKIFEKFYRVPQEADIPGTGLGLYLVRQFIL
                     MMNGRAWVEPREQGNCFVVTLPFG"
     gene            2800..4182
                     /locus_tag="PKO06_03145"
     CDS             2800..4182
                     /locus_tag="PKO06_03145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV68669.1"
                     /translation="MRILIVEDDHQQAENLARLLETMGNSVLFRTDPVQGLAAFRDEE
                     FDLTILDMRMPGIDGLTILKRALELRPDSRIVILTAFGTIDTAVEAMKRGAFDYLVKP
                     VDPERLKQILGLTADANRLSRMAGLSAPAGPEPNDFIALSPAMQRVRDLIRRVAAMNT
                     TVLIHGETGTGKELVARAIHANSPRRQKAFIAVNCANLQGNLLESELFGHEKGAFTGA
                     DSRKLGKFELASGGTLFLDEVGEIPLELQAKLLRAIQEQEIERLGGVKPVRVDVRLVS
                     ATNRDLEKCVEAGKFRQDLFYRLNVFPLTLPPLRRRRDEIGALADHFLQEWSEANGLM
                     RMTLAPDALDWLKEQPWPGNIRELRNLLERAAIIDEDGTLTRDDLLRNGLESGDDDAP
                     MMNGSGTPVEAEPLVHRLADQERGLISAALEKTHGNVVAAARDLGLPRRTFYDKLKKL
                     GIEPARYRHP"
     gene            4319..>6034
                     /locus_tag="PKO06_03150"
     CDS             4319..>6034
                     /locus_tag="PKO06_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV68670.1"
                     /translation="MTEQTWLQKTIRNVLVGGMTACWLMSATVAWSAGEPPILDRLNQ
                     LARDIHMSQKAVSGSGNQSHESAGAFAKEAATAADLQKAIFRVNTVDEAVAARETIQK
                     FGYRVTSTAERKAYQQAMNLLESRLKFLAQSESREFVPVVQSLEPLNKEIERLSFSVE
                     SGPGSRVKTIAAQADRARSASRTDVAGRLGFEVKNGKTSFAVSAPDATSVNLVLFNKP
                     EEKTGAGYAMKKDAAGVWRCSIDGELYGKYYGFAADGPNTPGFLFDPKRLLSDPCALA
                     NVDHDGKSIVVKTDFTWTDKGFKPPSAKDVIIYEAHVKDLTAHPSSGVPADLRGKYLG
                     ILEGQVLNHLKDIGVNAVEFLPLQEFDNNFAGHMNHWGYMTTHLRAPETACATGKKGE
                     AVKEFKQMVNGLHNAGIAVIMDVVYNHTGEGNEKGMPLNFKGLDNPGYYRLCDDKRFY
                     WNGTGCGNEFRSDHPVTRRYIVDSLKYWSKEYHIDGFRFDLGTILDKDTMTAIMNELP
                     ADTIIVAEPWAADWKRNQWGKSDFRNTKLGKWNDDFREKIREFVKGHGNRNDVMTVLA
                     GSCFWW"
BASE COUNT         1167 a         1775 c         1786 g         1306 t
ORIGIN      
        1 cggcactcag agcgcgtgga actgtctgac aaacgggttt ccaagtgccc gtcttacatg
       61 gaaaaagttt ccgtttgccc tgtcatgaaa gacccttgct gcggcggctg attccgggta
      121 cactgaaaat ctcatgtcgt tcccacttgt ctatcagcgc cccgagtttg gcaaactctg
      181 gctggcgcaa ctgatcgcat ttatcggcga tggcatcttc cagatcgcca tggtcatgtg
      241 gctgattcag aagacgaact ccggaaccct cgtcggcctg atcatcagtg tcagtttcct
      301 gcccgcggtt ctgatcgggc cgtttgccgg cacgctggcc gaccgtctgc cgccgaaagt
      361 gctgttgatc ggggccgatg tctctcgcgg tcttctgctg gcgatctttg ccggcgtcgc
      421 cggaaacgat ctgcttgaag tctggcacct gtttctcatc tgttcgctcc tggccacggc
      481 cggagtgttt cacagcccca cgacgctggc ggtcatacca cgtctggttt cacctgatca
      541 gatcgatggt gcgatggctc tgcacaccgt ggttcgcgat ctgtcgaaac tgatcggtcc
      601 ggcgctgggc ggcttcatcg ttgctcgctg gtccgtaccg gttgcctttg cttgtaatgc
      661 ttttgccctc ttcttttcag cggcatgcat tgcgatgatg tccattcccc cgcgcaccgc
      721 gaccggcggg gacaagcagg tcctgacgca gctgtgggaa ggcctggcct acgtgcgccg
      781 ccaggacacc atttttcgca tgctgtccgg gtttgccttt ctcaacctct ttgccgctca
      841 gattctcgtc ctgctgccgt tgaccgtgat gcagctgctg ctgcgcgatt cggtagcgct
      901 gggtttctgt gaaggggctc tcgcgctcgg ctcagtcgcg atcggctttt tcttcgtgca
      961 gattctgacg cgcattccga cctcaaagct gtttctgggt tccatggctg gttccggcgt
     1021 gctctttctc gtattcgccg gtaatgtccg gttctggaaa acccttgcca gcctgttcgc
     1081 cccggccgca acggcagaag ccatggtcgg tgccccgggg tgggggagct gggggcttct
     1141 cgtggctccg tatcccacct tcatcgttgg cctctttctc ctcgggggct gcttcgccgc
     1201 cgtgaacatc gccgccttga ccatttttca gaagattgtc gcgcccgaca tgaaggggcg
     1261 attcttttcg ctggtcgaag cgctcagtta tgtgtcactg ccgctggctt cggctttggc
     1321 cgggtatctc gccgatcacg ccggcattcc tgtcacctat gtgatcgggg gtgtcggtct
     1381 tttgatcctg acctggcgct tctcccgaat tccgggtctg acctcgttcg accaggccga
     1441 agcggcagcc ggtgctccgt ctccatcacc cgacgagaca aatcaaccag cacaaaaccg
     1501 tgaaccggag aacgtgttgc cgtgagtcga ccggtacgac aggttcatct gtctctgctg
     1561 gccggattgt tgcccgccgt ctttctgata ctggccctgc tggcctataa tgctctgatt
     1621 gaacgtatgc agagcgggtt gcatgaacag cggtcgctgg cgctccgcac ccttccggct
     1681 gccaccatcg cgcaacagaa acactacagt tgtgtggctc tgaccgccga tctggccacc
     1741 ttcaccatcc tgaccggtga ctggccgctg gcgctggaca acacggaaat acgccgtcta
     1801 cgcgagatgc tcggttcggg aaccgaactg gggatcgaga tcgagctggc tgatctcggt
     1861 ccggcgtttt tctggctggt acccatcgaa gggggctacc gtgccgcggt gcagagtcga
     1921 ctctcgtttc gttcgcgggt cgagtctttt cgcgccctga tctggatcgg cgctctgctg
     1981 ctcgccggga tcgcgttttt cgtctatgcg ctgctggccc gcaagctctc cgacgtcttt
     2041 tccgaaatgg aagcgaagaa tctcgaactg gaacgggcca atcgtcacct cgaagagctc
     2101 ggcaagctga aaagcaactt cctcgcgctc gtgtcgcacg aactgcgtac cccgctggcc
     2161 cgcatggccg gtcatattca gttgatcaag gctcaggaag aacagctgcc acccgagcaa
     2221 cgtcgacgct tcgaagagat gtcgctcgac gtcgaagaat tgagccgcat gaccaaaaac
     2281 gtgctcgatc tgacccgcct gcagagcgaa gatctgtcgg cgcggcttga cctgtcgcag
     2341 gtgtcgccgc tggtgcgatc ctgcgtcgaa cgctcgcgac ggggggcggc ggcacgtgga
     2401 ctgaccctcg aatgcgaaac gcccgaaacg ccgccggtca gtcatgatcc gtatctgctc
     2461 gagcgaattc tcgacaacct gctggtgaac gcaattaagt actcgtttcc tgataccgtc
     2521 attcgggtca ctctggttga gcgtgaatcg aacgttgaaa tctgcgtcga aagtcggggc
     2581 gccgtgattc cctcggctga tcgcgaaaag atcttcgaaa aattctatcg ggtgccgcag
     2641 gaagcggata ttcccggcac cgggttgggt ctgtatctgg tgcgacagtt tatcctgatg
     2701 atgaatggtc gggcctgggt cgagccgcgc gaacagggca actgcttcgt ggtcacgctg
     2761 cccttcggtt aagcgccgaa gtgtgataca ctccccccca tgcggattct gatcgttgag
     2821 gacgaccacc agcaggcgga aaacctcgcc cggttgcttg aaaccatggg caactcggtg
     2881 ctgtttcgca ccgatccggt gcaggggctc gctgcttttc gcgacgaaga gttcgatctg
     2941 accattctcg atatgcgcat gcccggcatt gacgggctga cgattctgaa gcgcgcgctc
     3001 gagctgcgcc ctgattcgcg gattgtgatt ctgacggcgt tcggcacgat cgatacggcg
     3061 gtcgaggcca tgaaacgagg ggcgttcgac tatctggtca aacctgtcga cccggaacgc
     3121 ctgaagcaaa ttctcgggtt gaccgccgat gcaaaccggc tctcgcgcat ggccggtctg
     3181 tctgctcctg cgggccctga gcccaacgac ttcatcgcgc tcagcccggc gatgcaacgg
     3241 gtgcgcgatc tgatccgtcg cgtggcggcg atgaatacga cggtgctgat tcacggcgaa
     3301 accggaaccg gaaaagagct ggtcgcgcgg gcgattcacg ctaacagccc gaggcgccag
     3361 aaggcgttca tcgccgtcaa ctgcgcaaat ctgcagggaa atctgctcga gtccgagttg
     3421 ttcggccatg agaagggcgc ctttaccggt gctgacagcc gcaaactcgg caaattcgaa
     3481 ctcgcgagtg ggggcacgct ctttctcgat gaggtcggcg agattccgct cgaactgcag
     3541 gccaagctgc tgcgcgccat tcaggaacag gagatcgagc gactgggcgg ggtgaaaccg
     3601 gttcgggtcg atgtgcgcct ggtctcggcc accaaccgcg atctcgagaa gtgcgtcgag
     3661 gccggcaaat tcagacagga tctcttctac cgcctcaatg tctttccgct gaccctgccg
     3721 ccgctgcgtc gccgccgcga tgaaatcgga gccctggccg atcatttcct gcaggaatgg
     3781 tctgaagcga atgggttgat gcgcatgacg ctggcccctg atgccctcga ctggctgaaa
     3841 gaacagccct ggcccggaaa catccgtgag ctgcgcaacc tgctcgaacg agcggcgatc
     3901 atcgatgaag acggaacgtt gacccgcgac gatctgcttc ggaatggcct ggagtctggt
     3961 gatgacgatg caccgatgat gaacggctcg ggaacacccg tcgaggctga accgctggtg
     4021 caccgtcttg ccgatcagga acgcgggttg attagcgccg cgctcgaaaa aactcacggc
     4081 aacgtggtag ctgctgcccg cgacctcggt ctgccccgcc gtacgttcta cgataaactc
     4141 aagaaactcg gcatcgaacc cgcacgctat cgtcacccat aaccccgcgc gttctttttt
     4201 tccatcacgt tttcatctga cgtgattctg ggtcttccgg ggtgcccggt gaaacgggta
     4261 taatgaaaaa tcaccgcttg gtgcggcggt atgggatttt ccattcagga ggcagggaat
     4321 gacggaacaa acctggttgc agaaaacgat tcgcaacgtt ctggtcggtg gcatgaccgc
     4381 ttgttggctg atgtcagcga ccgtcgcctg gtcggcgggt gaaccgccga ttctcgatcg
     4441 cctgaatcag ctggctcgcg acattcatat gtcgcaaaag gcggtttcgg gctctggaaa
     4501 ccagagtcac gaaagtgccg gcgccttcgc gaaagaagcg gcgaccgccg ctgatctgca
     4561 gaaggcgatt ttccgcgtca atacggtgga tgaagcggtt gccgcccgcg aaaccattca
     4621 gaaattcggg tatcgcgtca cctcgaccgc cgaaaggaaa gcctaccagc aggcgatgaa
     4681 cctgctcgaa tcccgcctca agttcctggc tcagtctgaa agccgtgaat ttgtcccagt
     4741 cgttcaaagc ctcgagccgc tcaacaagga aatcgaacgc ctcagtttct cggttgaaag
     4801 cggccccggc agtcgggtca agacgatcgc cgcgcaggct gatcgcgccc gatctgcctc
     4861 tcgcactgac gttgcggggc gcctggggtt cgaagtgaag aacggcaaga cctcgtttgc
     4921 ggtttccgca cccgatgcaa cgtcagtgaa tctggtgctg ttcaacaagc ctgaggaaaa
     4981 aaccggcgcc ggctatgcga tgaagaaaga tgccgcgggt gtctggcgtt gctcgatcga
     5041 tggcgaactc tacggcaaat actacgggtt tgccgctgat ggtccgaata cccccggctt
     5101 tttgttcgat ccgaaacgtt tgctttctga tccttgcgcg ctggcgaacg tcgatcatga
     5161 cggaaagagc attgtggtca agaccgattt cacctggacc gacaaagggt tcaaaccgcc
     5221 gtcggcgaaa gacgtcatta tctatgaggc tcatgtcaag gacctgacgg cgcaccccag
     5281 ctccggtgta ccggctgact tgcgcggaaa atacctcggg atcctcgaag ggcaggtgct
     5341 caatcatctg aaagatatcg gggtcaacgc ggtcgagttc ctgccgctgc aggagttcga
     5401 caacaatttc gcggggcaca tgaatcactg gggctacatg acgacgcacc tcagggctcc
     5461 tgaaaccgcg tgtgccaccg gcaaaaaggg cgaggcggtc aaggagttca agcagatggt
     5521 caacggcctg cacaatgccg gcatcgccgt gatcatggat gtggtctaca atcacaccgg
     5581 cgaaggcaac gagaaaggga tgccgctgaa cttcaagggg ctcgacaatc ccgggtacta
     5641 tcgcctgtgt gacgacaagc gcttctactg gaacggaacg ggctgtggca acgaattccg
     5701 atccgatcat ccggtgactc gacgctacat cgtcgacagc ctgaaatact ggtccaaaga
     5761 gtatcatatc gatgggttcc ggttcgatct gggcacgatt ctcgacaaag acacgatgac
     5821 cgcgatcatg aacgaactgc cggccgatac catcatcgtc gctgagcctt gggccgccga
     5881 ctggaagcgc aatcagtggg gcaagagcga cttccgcaac accaagctcg ggaaatggaa
     5941 cgacgatttc cgcgaaaaga ttcgcgagtt tgtcaaaggt catggcaacc gcaatgacgt
     6001 catgaccgtg ctggcaggtt cctgcttctg gtgg
//
LOCUS       DAORHD010000093         6014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13310_length_6014_cov_4.319517, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000093 DAORHD010000000
VERSION     DAORHD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13310_length_6014_cov_4.319517"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2564)
                     /locus_tag="PKO06_03155"
     CDS             complement(<1..2564)
                     /locus_tag="PKO06_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68671.1"
                     /translation="MRHTAAFLLGFQFLVRFGSTLLFIAAQTFFFRQAGVERLPALYA
                     VVNLAFIVLQVFLLPFFRQRAGRTLGHLLVAMPFLLVVGFLAGLRQSDLGVSTLLLLV
                     MVFELLFGQLFTQHLSETIPLQQGKSMLPMLSAFASISGIVAGITLRLLLAIYSLSEV
                     YGLGTLILGCACLAWLMTGRGDETHPSGDGPEPPTSLSESQAPVASSSEPLSPALKDL
                     LWLVLLLAGTNMFGKYWLDYQYSRGITRAFSTERDLASFMATFSSVTDLLIILTQMLL
                     TGRLLRQWKLSMALYFMPALVLCGCLGHLGVGSLASLLAVQFLYTFLVKTCFQPSLSL
                     VFSVFPAAWRGQVIARFGLMSSGAALITGLLLALGGNQWVETRSFLLLSLVFASCLWV
                     VHRLDRSYVSAIVSALGLSERETREDLEVLTRLDQATRHSWLKRHLQQESSRADAVTL
                     IPDLFPDQAAVQLLAYVTATESPKVREHALQALTRIADHQVPWIEAFLLDERLEVRTR
                     AGLLESLGCSEAGPRYLPVARRLLENPQLRLQAAAAALIGKHENEERVLVQALERVWN
                     WVENTADTLSQGAGVAVMGSIGHEAFVPALTRVASQGAGNLSLEAVVSLSRIPSPETR
                     AALIHLQGQATDARVREQAEKESQRFERLSEQDMISILGSLNPDERQRLLQQTQWQHD
                     ARRLHLMRLSAAEPDLQTRRALLRFWVIADQRLMVSVLELDRDLEKQGSLVTLLTTHL
                     LMTVGLRARFLIPLVDALAENQAWSTCESLLLALLSRVALEDACGEQVGRSHDSARWS
                     RWRAWLCDLANRRSAGRFPSDLSALAGDVRFTTSLLHEVLLAAFNRQTAERLACVLL"
     gene            complement(2561..3763)
                     /locus_tag="PKO06_03160"
     CDS             complement(2561..3763)
                     /locus_tag="PKO06_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV68672.1"
                     /translation="MTGRGRLRWRLAFAFLTVGLAPILILGWISLRVAEGYLRQTTLT
                     RDQTLLNAVTHGLELFVRGRYDQLAHLAQQPTVQSLDAKRAAPLLRDSSSRLPFFDGL
                     FLLDATGTVVARAGGFARTLQQHSDLEQRFLDPYRDASRWSRDREPIVRHPDAEWRAR
                     LLILRAPVSDFLRPEQQNGLLVGVMALEGFEWQEILEAFQPNDREYVCILSESGEILT
                     RTGNGLAESATRFALGSLAAAASERRQAKTVEYLHQGRRDLLTISFSPLIGGWLLCGR
                     PADEAFRLLYQLRETWGVALLFTLVLSLAAVLLLSQMLAQPILSLTLGLQELREGRYG
                     YRVTTESDDELGTAARALNDLAAFLQRRYLIGSIWERFRGHRDTPSSPIVSETTPARS
                     EPGSRVDP"
     gene            complement(3786..3959)
                     /locus_tag="PKO06_03165"
     CDS             complement(3786..3959)
                     /locus_tag="PKO06_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68673.1"
                     /translation="MGEKKRTSGIVDRIEGNVVIVVVRDPQNPDDTIEVAIPRQRIKK
                     TDLQEGDRVTVLL"
     gene            complement(3980..>6014)
                     /locus_tag="PKO06_03170"
     CDS             complement(3980..>6014)
                     /locus_tag="PKO06_03170"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNV68674.1"
                     /translation="PLKAIPTLLLFLLPATWWHRSRLGWKGLIGIWTLLVLYSWFVLQ
                     ELVVAVMIFPTFHLLAYRTSRFIDQRYPGRLRDVSTERKVLPATPEGLLGEGIFSEMV
                     DRLWQSFCGIMAQSVGENTPEEVASEKVEVLQMQFQDPRQMSRALTDLVRRDGSFKEK
                     PFLKRVEDVFWKVQKALYSQDLAIVQPFVSDGLFEQLQVRVEEQQAVNIRYQVSDMGI
                     YDLRFVQVHSDHSFDTMHVLVRAYAHEEQVDESRGERQRTEGGRRQFDEVWAFLRRPD
                     AKTLERPGLLEGFCPNCSAPIEIGQAARCKACDAFLRAGTHDWVLFQITPACEWHYGE
                     PEEIPGMRDLRMVDPTFTVQHIEDRSAVIFWKERQAERTGQVEPLRRFVLPESCDRLE
                     QRWRELGDDREYQEFCALGKVRLKAILSDPDWDRLYVETVWSGVPKALRAGKARTLST
                     NPRFFRKILVLRRRHGLATCLDSALSSSHCHSCGAPAKAGFNSRCDYCQAILNEGDTA
                     WVLDWWVGEDDPDLLKALSQRVEQKVVVPQVFAEGGSRLDTVAALAQMMLADGVVDAG
                     EMKLLQTFAAGHGIPPVRVEQVIQSLREGWIHLPKLDKRQDAHHLLQSAVRMALADGV
                     IVEAEMEMLHQLARHLGYSEADVKYLLARHHKKEYQDQRRLEQPRCFSGKDQE"
BASE COUNT         1435 a         1823 c         1648 g         1108 t
ORIGIN      
        1 agcagaacgc acgcgagacg ctccgccgtc tgacggttga acgcggccag cagcacctcg
       61 tgcagcaacg aagtcgtgaa gcgcacgtca cccgcaagtg ctgagagatc agaagggaac
      121 cgaccggccg accgtcgatt cgcgagatca cagagccagg ctcgccaacg tgaccagcgg
      181 gcagaatcat gagagcgccc aacctgttca ccgcaggcat cttcgagagc aacgcgggac
      241 aacagagcca gaagcagcga ctcacaggtt gaccacgcct ggttttcggc cagtgcatcg
      301 accaggggga tcagaaatcg cgctcgcagg ccgacggtca tgagaaggtg cgtcgtcagg
      361 agagtcacga gagagccctg cttttcgaga tcacgatcga gttcgagaac cgacaccatc
      421 agacgctgat cggcgatcac ccaaaaacgc agaagtgcac gacgcgtctg caggtcaggc
      481 tcagcggcac tcagccgcat cagatggaga cgtcgagcat cgtgttgcca ctgggtttgc
      541 tgtaatagtc gttgtcgttc gtcagggttg agggaaccca gaatagagat catatcctgt
      601 tctgagagcc tttcaaatcg ttggctctct ttttccgcct gctctctcac tcgcgcatcg
      661 gtcgcctgtc cttgcaggtg tatgagagcg gctcttgttt cggggcttgg gattcgtgac
      721 agcgagacaa ccgcctcaag cgacagattg ccagccccct gcgaagcgac gcgtgtcagc
      781 gcgggaacga aggcttcatg tccgatcgag cccatcacgg ccacaccggc tccctgagac
      841 agggtgtcgg ccgtattctc aacccagttc catacacgct ccagggcctg aaccagcacc
      901 cgttcctcat tttcgtgttt gccaatgagc gctgccgccg ccgcctgcag acgtaactgc
      961 ggattctcga gcaaacggcg ggcaacgggg agatagcgcg gcccggcctc ggaacacccg
     1021 agactttcca ggagacctgc tcgagtgcga acctcaaggc gttcatcgag caaaaatgct
     1081 tcaatccaag gaacctggtg gtcagcgata cgagtcagag cctgcagcgc atgttcgcgc
     1141 accttcggtg actctgtcgc ggtcacgtat gccagcaact ggacagcggc ttgatccggg
     1201 aaaaggtccg ggatcagggt taccgcatca gcgcgactgg actcttgttg aaggtgccgc
     1261 ttcagccacg agtgtctcgt tgcctggtcg agccgcgtca gcacctcaag atcttctcgc
     1321 gtttcgcgct cgctcagccc aagtgccgag acgatggccg acacataaga tcgatcaagc
     1381 cggtgaacca cccacagaca cgacgcaaac acaagactca ggagcagaaa cgatcgtgtt
     1441 tccacccatt ggtttccgcc caatgccagg agaagtccgg tgatcagagc agctccagaa
     1501 gacatcagac cgaatctggc aatgacttgc ccacgccatg cggcagggaa cacggagaag
     1561 acgagcgaca gcgaaggttg aaagcaggtc ttgaccagaa aggtatacag gaattgaacc
     1621 gccaggagac ttgccagact gccgacgcca agatggccca ggcaaccgca caagaccagc
     1681 gcaggcatga aatacagtgc catcgagagt ttccactggc gtaacagtct gcccgtcaac
     1741 aacatttggg tcaaaatgat cagcaagtcg gtcactgatg agaatgtcgc catgaatgac
     1801 gccaggtctc tttccgttga aaacgctcgg gtaatgccac gcgaatactg ataatcaagc
     1861 cagtatttgc caaacatgtt ggttccggcc agcaggagta ccaaccacaa caaatccttc
     1921 agagctgggc tcaaaggttc ggaagaggat gccacgggag cttgagactc cgacagactc
     1981 gtcggcggct ccggcccgtc cccggaagga tgggtctcat caccacgccc cgtcatcagc
     2041 caggcaaggc aggcacagcc cagaatcaac gttcccaacc catacacctc ggacaaactg
     2101 taaatggcca acagcaatcg caacgtaatg ccggccacaa tgccactgat cgaggcaaac
     2161 gccgacagca tcggcagcat ggattttccc tgctgcagcg ggatcgtttc actcaggtgc
     2221 tgtgtgaaca actgcccaaa gagtagttcg aaaaccataa ccagcaacaa cagagtcgag
     2281 accccaagat cgctctgacg caaacccgcg aggaaaccaa caaccagtag gaacggcatg
     2341 gcaaccagca gatggcccag agtccgcccc gcccgctggc gaaaaaacgg caacagaaaa
     2401 acctgcagaa caatgaaggc cagattcacg accgcgtaga gcgcaggcag gcgttcaaca
     2461 ccggcctggc gaaaaaagaa ggtctgcgcc gcaatgaaaa gcagtgtcga cccaaagcgc
     2521 accagaaact ggaaaccgag aagaaatgcg gccgtgtgcc tcatggatca actcttgatc
     2581 caggctcgga gcgtgccggg gttgtctctg acacgatcgg cgaggacggg gtatcccggt
     2641 gaccccggaa tcgttcccag atgctgccaa tcagatatcg acgctggaga aacgcggcca
     2701 gatcattcag agctcgtgcc gccgtaccca gctcatcgtc agactcggtc gtcacccgat
     2761 agccgtatcg tccctcgcgc aactcctgta aaccaagggt gagcgacaag atgggctgag
     2821 ccagcatttg tgacagtagc agcacggccg ccaaactgag taccagcgta aagagcaacg
     2881 ccactcccca cgtttcacgc agttggtaca acagccgaaa cgcttcgtcg gccggacgtc
     2941 cacacagtaa ccagccaccg atcaaggggc tgaacgagat cgtcagcaga tcgcgacgac
     3001 cctgatgcag atactccacc gtctttgcct gccgccgctc ggatgccgct gccgcaagac
     3061 ttcccagcgc aaagcgcgtg gcagactccg caagaccgtt tcccgtgcga gtcaggatct
     3121 ctccagactc ggacaaaatg cagacatact cgcggtcatt cggttgaaac gcttcgagaa
     3181 tctcctgcca ctcgaatcct tcgagagcca tgacgccgac cagaagcccg ttttgttgct
     3241 cggggcgcag aaagtcgctg acgggggcac gcagaatcaa caggcgtgcg cgccattcgg
     3301 catccgggtg acgaacgatc ggctcacggt cacgagacca gcgactcgca tcgcgatacg
     3361 gatccaggaa tctctgttcc aggtctgaat gttgctgcag ggttcgggca aacccaccgg
     3421 cacgcgccac caccgtaccg gttgcgtcga gcagaaacaa gccatcaaaa aaaggaaggc
     3481 gagatgacga atctctcagt aacggtgctg cacgtttcgc atcgagactt tgcaccgtcg
     3541 gttgttgcgc cagatgcgca agttgatcgt atcgacctcg gacaaacaac tcaaggccat
     3601 gggtcacggc attcagcagt gtttgatcac gcgtcagggt tgtctgtctc agatacccct
     3661 cggcaacccg cagggagatc catcccagaa tcagaatcgg tgccagtccg acggtcagaa
     3721 aagcgaacgc cagacgccaa cgtagacgtc cacgaccagt catgcgttgt tttcctcgac
     3781 cgaaatcaca gcagcaccgt cacccggtcg ccttcctgca ggtcggtttt cttgatacgt
     3841 tggcggggaa tcgccacctc gatcgtatcg tccgggtttt gcggatctcg cacgacgaca
     3901 atgaccacat ttccttcgat gcgatcgaca atccccgagg ttcgcttctt ttcacccata
     3961 gccctcattt ccgttacgct cattcttgat ctttgccgga gaaacaccgt ggttgctcca
     4021 gtcggcgctg atcttgatat tcttttttgt gatgacgtgc cagaagatac ttgacgtcag
     4081 cttccgagta gccgagatgt ctcgccagct ggtgcagcat ctccatttcg gcctcgacaa
     4141 tcaccccgtc ggccagtgcc atgcgtactg ccgactgcag tagatgatgc gcatcctgtc
     4201 gtttgtccag tttgggcaga tgaatccagc cctctcgcag ggactgaatc acctgttcga
     4261 cgcgaaccgg cggaatgcca tgccccgcgg caaatgtctg tagcagcttc atttctcctg
     4321 catcgacgac tccgtcggcc agcatcatct gggccagtgc cgccacggta tcgagtcgcg
     4381 aaccaccttc ggcaaacact tgcggaacca cgaccttctg ctcgactcgc tgactcagag
     4441 ccttgagcag atccgggtcg tcctcgccaa cccaccagtc gagaacccag gcggtatctc
     4501 cctcgttcag aatcgcctgg cagtagtcgc atcgactatt gaaccccgct ttggccggag
     4561 cgccacagga atgacagtgg gagctggaaa gagcggaatc aagacaggta gcgagaccat
     4621 gccgccttcg gagcacgaga atcttgcgaa aaaatcgcgg attcgtcgat agtgtgcggg
     4681 ccttgccggc gcgcaacgct ttcggaactc cgctccacac ggtctcgaca tatagtctgt
     4741 cccagtcagg atcggagaga atcgccttca aacggacttt tcccagggca caaaattcct
     4801 ggtactcgcg atcatcaccc agttcacgcc atcgttgttc gagccgatcg caggattcag
     4861 gcaggacgaa acgacgcaaa ggctcgacct gaccggtccg ttccgcctgc cgttccttcc
     4921 agaaaatgac cgctgagcgg tcttcgatat gctgcacggt gaaagtggga tcaaccatac
     4981 gcagatcacg catgcccgga atttcttcgg gctcgccata gtgccactca caggcgggag
     5041 tgatctgaaa caacacccag tcatgcgttc cggctcgcaa gaaggcatcg caggccttac
     5101 accgggcagc ctggccgatc tcgattggcg cgctgcagtt cggacagaag ccttcgagca
     5161 acccggggcg ttcgagtgtc ttggcatcgg gacgacgcag gaaagcccag acctcgtcga
     5221 actgtcgccg gcctccctcg gttcgctggc gttcgccccg actttcgtcc acctgttcct
     5281 catgcgcata tgcacgcacg agaacatgca tggtatcaaa ggaatgatca ctgtgaacct
     5341 ggacaaatcg cagatcgtag atgcccatgt cgctgacctg ataccgaatg ttgacggctt
     5401 gctgctcctc aacacgcacc tgcaactgtt cgaagagccc atcagaaacg aatggctgca
     5461 cgatggcgag atcctgtgaa tagagcgctt tctgaacttt ccagaacacg tcttcaacgc
     5521 gtttgagaaa gggtttctcc ttgaatgagc cgtcgcggcg aaccagatcg gtcaaagcac
     5581 gagacatctg gcgcgggtcc tgaaactgca tctgcagcac ttcgactttc tcgctggcca
     5641 cttcttcggg agtgttttct cccacggact gtgccatgat gccgcagaac gattgccaaa
     5701 ggcgatcgac catttcggaa aagatgccct cgcccagcag accttcgggg gtcgccggca
     5761 agaccttccg ttccgtcgac acgtcgcgca gacggcctgg ataccgctgg tcgataaagc
     5821 gactggtacg ataggcgagg agatgaaagg tcggaaaaat catgaccgcc accaccagtt
     5881 cctgcaacac gaaccaggaa tagaggacca gcagggtcca gatgccaatc agtcccttcc
     5941 agccgagtcg agaccgatgc caccaggtcg ccggcagcag gaacagcagc agcgtgggaa
     6001 tagctttcag gggg
//
LOCUS       DAORHD010000094         5992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13381_length_5992_cov_2.527876, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000094 DAORHD010000000
VERSION     DAORHD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13381_length_5992_cov_2.527876"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PKO06_03175"
     CDS             complement(<1..788)
                     /locus_tag="PKO06_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68675.1"
                     /translation="MSTATKEYTQKRRIGIVCTVGTRDLTLDGQTAEKLGKAGNTEFA
                     SPRKLGKEILDDWDNLSKRIAFPIIQPVIDSAFERARSSQGEIVYLLLVGTDQPTLIE
                     ETHREKDTAYFTEVAVNYYSQQSQRPSKSFQAKRLLYPMNPTRMDIAWNYLQQKASKE
                     CFTELRNCDQVFLSTTGGVPGLNQALLMFLFSEVCGRNLSFVHVDEDLQRPIPIEIVQ
                     HLTRLETIHRIEAYVETFDFAAMARELLEEDQEGDHRYRRHPCHP"
     gene            1138..1575
                     /locus_tag="PKO06_03180"
     CDS             1138..1575
                     /locus_tag="PKO06_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68676.1"
                     /translation="MCDRGLKATVEKKQWLFESLFGGTNPTRQARFGEVWWVRDSAWG
                     RLEPLRHLNDDAKLHRGLCIRPQARMMAFGTSKIAQARKSKSRLFEVPAGAYDGGDRP
                     SVFLLDYALPALWQDCKLNHEPALLREDLIEKLRLKLEKRGKQ"
     gene            1572..2651
                     /locus_tag="PKO06_03185"
     CDS             1572..2651
                     /locus_tag="PKO06_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68677.1"
                     /translation="MSAVELGTQKGESRRPSPLGEALERWWQRPDSRNWADFLMALYE
                     YIPKVLRRVCKNDDLHEADNQSLEELTHFVYERLLRELMLPEANAVDVNVMEDYSDEI
                     RLISKDPLTRQKLALMRTTHVSAQTVVYLQDRILNYGKARLRKTWKSYKKRDHIPDEP
                     DEAEDERPTEKLSDGTAVRVDDVVIIAPSSSLRKKADEITVQIWKSKPKKAQLEAWII
                     QLCLKHPLEMANREVPQSFFRRLNVVDDVGERRKVERSARTSEATDDGDKQAVQAMTI
                     AKFLNLKRPTVFERVTRFDEFLREELELMCTSEDQIAGVSDYLLTLWIRKLAEEDPSF
                     WENPEHGRSETADTAPSGLIEEKLP"
     gene            2648..3742
                     /locus_tag="PKO06_03190"
     CDS             2648..3742
                     /locus_tag="PKO06_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68678.1"
                     /translation="MNKTTACLNAASLQRFIEQKRAGKTDADTLRHVERCRRCSLLVA
                     AAKDYDEKAMLAEHARVEKLFQQAKPKTVEPGQIWRLEKQPDQSRPLGIITRGPEMSI
                     DLGDPDVRFVRARLDYLHNQIDENEDVFVHADESPLRVRYLLETWNERPIPTSMLESY
                     VGKLAPSAWQRLESALTSTSPAKPLPRAVAAFRERCVQESADLSRMVMHKLARILGDE
                     EKASQKKPTRMVQTTTAEAGNASSLPWIFRLSGMTEELFSSDNEVLPLAAADQTHDQA
                     FETWVNVTMKKIRSDASCPVVVRRAAVDTMCFKAKKTVKQSLRLELRSERNRLLKTFM
                     MIAGEIRIRVDEVKDWSKVAAVKVVHDEDE"
     gene            3726..>5992
                     /locus_tag="PKO06_03195"
     CDS             3726..>5992
                     /locus_tag="PKO06_03195"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68679.1"
                     /translation="MTKTSDQYLQTAAATARARLKLILRPQQASPAVVGDALSSRFKT
                     DDPELTEFRRWFNALPVPIEAVLHLYDSHHDELYGPLGDLLLACTTKPSMTACFPYRL
                     DLLKRWARAFATRHDIDASNMLTSMIAYKEHLLTIKSDAPSGHASELPGLACPLVKEN
                     QFVIWETAFTGADAAPWKTLGICRRVAGTAGARRRGVEEAIETAYHLLKKENKQDFDR
                     ECCLPAYHLCDVLIEGESCGLAIWAALWLRLHGRSTILPWGPTMLTGKLDEQGLVHSI
                     GSLEQKVETALACGFKRVFVPAENLDALEDTHPFRRDARVLGVRSVDDVRQIILASDP
                     IPQAEALIKACVDGVVDPSESKTRETMEVWWRKQLEEGSLMQSFALVRQNLREQRRRD
                     KARCWRHMVRPLFGVFAAHLRQNQTEKIPRLLWSRLFQALVPDSVFLLHLPLLLDRYC
                     LGSRAEAYRLYTELGLRPVRHDLDLALALAWGNELFQEDSVNVWRKPEFRSQFPLMCW
                     YLFRDPLKAIESILSWPSRSPQEEQWRHSFFQQLKRLSPLLYACKTRRSDAKTATCLR
                     ADAPGGLAELRLMPAELVPIDRRPALMLKIRSAYGDELSKGEVDILRTHFLAHLESRF
                     DKPTALRMTRLFWDDAHPQSVILPDEKHPGHQMIKRCLQEYRHSKDIRSKTTANASPA
                     SWMGRLSRAWNADTLMIKAQEAKTNDALVSTRDGIRELDKGLNQALWAMCRGKPVRGD
                     DALAFGDFANPALLF"
BASE COUNT         1385 a         1560 c         1717 g         1330 t
ORIGIN      
        1 gggtggcagg gatgacgccg gtatcggtgg tcgccttctt gatcttcttc gagaagttcg
       61 cgagccatgg ccgcgaaatc gaacgtttca acgtaagcct cgatgcgatg aattgtctcc
      121 aggcgtgtca gatgttgaac gatctcgatc ggaatcggtc gttgtaaatc ttcgtcaacg
      181 tggacaaatg aaaggttgcg accgcagact tcgctgaaga ggaacatcag cagggcttga
      241 ttcaacccgg gaacgcctcc agtcgtcgag agaaagacct gatcgcagtt tctcaactcg
      301 gtgaagcact ctttgctcgc cttctgttgc agatagttcc aggcaatatc catgcgggtc
      361 gggttcatgg gatacaagag tcgtttggcc tggaaacttt tcgacggcct ttgactttgt
      421 tgcgaatagt agtttactgc gacttccgtg aagtaggcag tgtccttttc ccggtgagtc
      481 tcttcaatca atgtcggttg atcggttccg accaatagca gatacacgat ttccccttgg
      541 ctcgatcggg ctcgctcgaa ggctgaatcg atgaccggtt gaatgatggg gaaggcgata
      601 cgcttcgata gattgtccca gtcgtcgaga atctctttac ccagtttacg cggactcgcg
      661 aactccgtgt ttccagcttt gcccagtttt tcagctgttt gtccgtcgag agtcagatcg
      721 cgcgtgccga ccgtgcagac aatgccgata cggcgtttct gtgtgtattc cttggttgcg
      781 gttgacatga catcctcctc gagtgcaatc tgtgggcagt gaccgttcgc catattcgct
      841 tctcccgctt tccgacaatg tatcactccg cttgctatac cgccagaaca tacaatattc
      901 actgctactg aaatatttga ctgaaattct gcagtagaac tgaaaacatt tatgcaactt
      961 cgattgttca ttgctggtgc gcattttggc ggtttgatcg gtttttagca cccgcggatt
     1021 ttctggaatg gcaagtactt ctgagaccgg cggaaaagtg atggttcggc gctgaaaaat
     1081 tggccggggt ggggtatcat gcgtgggtag ttcatctcag gggtgaggcg gagacgcatg
     1141 tgcgaccgag ggttgaaggc gacggttgag aagaaacagt ggctgtttga gagcctgttt
     1201 ggcgggacga acccgacacg gcaggcgcgg ttcggagagg tctggtgggt gcgcgattcg
     1261 gcgtggggcc gactcgagcc gttgcgtcac ctgaacgatg acgcgaaact gcatcgcgga
     1321 ctgtgtattc ggccgcaggc ccgcatgatg gccttcggca cttcgaagat cgcgcaggcc
     1381 agaaagtcga agagtcgcct gttcgaagtg ccggccggag cctacgacgg cggcgatcgt
     1441 ccgagtgtgt ttctgctcga ttacgctctg cctgcgctct ggcaggattg caaactgaat
     1501 cacgaacccg ccttgctcag ggaagacctg atcgaaaaac tgcgcctgaa gctcgaaaag
     1561 cggggcaaac aatgagcgct gtcgaactcg ggacgcagaa gggcgagtcg cggcgaccct
     1621 cgccgctggg tgaagctctc gaacgctggt ggcaaagacc cgacagccgg aactgggccg
     1681 attttctgat ggctctgtat gaatacattc ccaaggtctt gcgaagagtc tgcaaaaacg
     1741 acgatcttca cgaggctgac aaccagtcgc tggaggaact gactcacttc gtgtacgagc
     1801 gactcttgag agaattgatg ttgccagagg ccaatgcggt cgacgtgaat gtgatggaag
     1861 attattcaga cgagattcgt ctcatcagca aagacccgct gacccgacaa aaacttgctc
     1921 tcatgcgaac cactcatgtc agtgcccaga cggtcgttta tctacaggat cgcattctca
     1981 actacggcaa agccagatta cgcaaaacct ggaagagcta taagaagcgt gaccacattc
     2041 ctgacgaacc tgatgaggcc gaagacgaaa ggccgaccga gaagctttcg gacggaacgg
     2101 ctgtcagggt cgatgatgtg gtcatcattg cgccgtcttc ctcgctcagg aagaaggctg
     2161 acgagattac tgtacagatc tggaagtcaa agccgaagaa ggcccagctc gaagcgtgga
     2221 taattcagtt gtgtctgaaa cacccactgg aaatggcgaa tcgagaggtc ccacagagct
     2281 tctttcgacg actcaacgtg gttgacgacg tcggcgagcg gcgaaaggtc gaacgatcgg
     2341 cacgaacgag tgaggcaacc gatgatggag ataaacaggc ggtgcaggcg atgacgatcg
     2401 cgaaattctt gaacctgaaa cgcccaactg tctttgaaag ggtgacacgg ttcgacgagt
     2461 tcctgcgcga ggaactcgaa ttgatgtgta catcagaaga tcagatcgcc ggggtgtctg
     2521 actacctgct caccctctgg atcagaaaac tcgcagaaga ggacccctcg ttctgggaga
     2581 atccggaaca cgggcgtagc gaaacggcag atactgcgcc aagcggtctg atcgaggaga
     2641 aactgccatg aacaagacaa ctgcctgcct caatgccgca tctttgcaac gcttcataga
     2701 acagaagcgt gccggcaaaa cagacgcaga cacactgcgg cacgtagaac ggtgccgtcg
     2761 ctgttccctg ctcgttgcag ccgcgaaaga ctatgatgaa aaagccatgc tcgccgagca
     2821 tgcgcgggtc gagaaactct ttcagcaagc gaaacccaaa acggtcgaac ccggacaaat
     2881 ctggcggctc gaaaagcaac ctgatcaatc tcgaccgctt ggaatcatta ccaggggtcc
     2941 cgaaatgtcg atcgacctcg gcgatccgga tgtgcgtttt gtccgagcca gactcgatta
     3001 cctgcataat cagatcgatg aaaacgaaga tgttttcgtt catgctgacg agtcaccatt
     3061 gagagttcgc tatctgctcg aaacctggaa cgaacgcccc attccgacat ccatgcttga
     3121 gtcatacgtc ggcaaacttg caccgtccgc ctggcaacga cttgaatcgg cccttacgtc
     3181 gacatctcca gcgaaaccgt tgccgcgggc agttgccgct tttcgtgaac gttgcgtgca
     3241 agagagtgcc gacctgtccc ggatggtcat gcataaactc gctcgtattc tcggtgatga
     3301 agaaaaagca tcgcaaaaga aaccgacaag aatggtgcaa acaacaactg ccgaagcagg
     3361 caacgcctct tccctgccct ggattttccg tctttcgggg atgaccgaag agcttttcag
     3421 ttcggacaat gaagtgcttc cgcttgccgc cgccgatcag acacatgacc aggcctttga
     3481 aacgtgggtg aacgttacca tgaagaaaat cagatcggac gcatcatgcc cggttgtggt
     3541 gcgcagagcc gccgtcgaca cgatgtgttt caaggcgaaa aaaacagtga aacagtctct
     3601 tcgtctcgaa ctgcgatcag aaaggaatcg tctgctgaag acctttatga tgattgccgg
     3661 cgagattcgc attcgtgtcg atgaggtaaa agactggagc aaggttgccg ccgtcaaagt
     3721 cgtgcatgac gaagacgagt gatcagtacc tgcagacggc ggcggcaacg gcgcgagctc
     3781 ggctaaagct catcctccga ccgcagcagg catcgcccgc cgttgtcggc gatgccttgt
     3841 cgagccgctt caaaacagat gatccggagt taacggaatt tcggcggtgg ttcaatgccc
     3901 ttccggtgcc gatcgaggcg gttcttcatt tgtacgattc tcaccatgat gaattgtatg
     3961 ggccgctggg ggatctcctt ctcgcatgca cgacaaaacc atcaatgacg gcgtgtttcc
     4021 cgtatcggct cgaccttctg aaacgctggg ctcgagcttt tgcgacacgg cacgacatcg
     4081 acgcgtcgaa catgctgacc tcgatgattg cttacaaaga gcacctgctt acgatcaaga
     4141 gtgatgcgcc gagtggtcat gcgtcagagc ttcctggact ggcttgcccg ctggtgaagg
     4201 aaaaccagtt cgtcatctgg gaaaccgctt tcaccggtgc ggatgcggca ccgtggaaaa
     4261 cactcggcat ctgtcgaaga gtcgccggca cggctggggc acgccgccga ggtgtcgaag
     4321 aggcgatcga gacggcgtat cacctgttaa aaaaggaaaa caagcaggat ttcgatagag
     4381 aatgctgttt acccgcctat catctctgcg acgtgctcat cgaaggcgag tcgtgcggtc
     4441 tggcgatctg ggccgcactg tggctccgac tgcacggtcg atcgaccatt ctgccgtggg
     4501 gtccgacgat gttgacgggt aaactcgatg agcaaggttt ggtgcatagc atcgggtcct
     4561 tggaacaaaa agtggagacg gccctggcat gcggcttcaa acgagtgttc gttccggctg
     4621 aaaacctcga tgcgctcgaa gatacgcatc cgtttcgacg cgatgcgcgc gtgctgggtg
     4681 tccgttccgt cgatgacgtg cgacagatca ttctggcatc tgatccaata ccgcaggctg
     4741 aagcattgat caaagcctgt gttgacggtg tggtcgatcc gtccgagtcg aaaacccgtg
     4801 aaacgatgga agtctggtgg cggaagcaac tggaagaggg atcgttgatg cagagttttg
     4861 cgctggttcg acaaaacctg cgcgaacagc gccggcggga taaagctcga tgctggcggc
     4921 acatggttcg accgttgttt ggtgtttttg ccgcacatct acgccaaaac cagaccgaga
     4981 agataccccg tcttttgtgg agccggttgt ttcaagcgct tgtgcccgac tctgtgttcc
     5041 tgttgcatct gccactgttg ctcgatcgtt actgtctggg aagtcgcgcc gaagcctatc
     5101 gtctctatac cgagctcgga ttgcgtccgg ttcgtcatga cctggacctt gcattggcac
     5161 tcgcctgggg caacgaactt tttcaggaag acagcgtcaa tgtctggcgg aagccggagt
     5221 ttcgttcgca gtttccgttg atgtgctggt atctgttccg tgatccgctc aaggcaatcg
     5281 aaagcatttt gtcgtggccg agccggtcgc cacaggaaga acagtggcgc cactcgttct
     5341 ttcaacagtt gaaacgactt tcccctcttc tgtatgcctg taagacacgt cgttcggatg
     5401 ccaaaaccgc gacgtgtctt cgtgccgatg cgcccggcgg tctggcggag ttgcgtttga
     5461 tgccggcaga actggtgccg atcgatcggc gcccggctct catgctcaag atacgaagcg
     5521 cgtacgggga cgagttgtcg aaaggtgaag tcgacattct acgcacgcat ttcctggccc
     5581 atcttgagtc gcggttcgac aaaccgacgg ctctccgcat gacaagactg ttctgggatg
     5641 atgcccaccc gcagtctgtc atacttcctg acgaaaaaca ccccggtcac cagatgatca
     5701 aacggtgtct gcaagaatat cgccacagca aagacatacg ttccaaaacg actgcgaatg
     5761 cttcccccgc aagctggatg gggagattgt ccagggcctg gaatgccgac acgctgatga
     5821 tcaaggcaca agaagcgaag acgaatgacg ctctcgtgtc cacgcgcgat ggtattcgag
     5881 aactcgataa aggtttgaat caggcattgt gggccatgtg ccgcggtaaa ccggtgaggg
     5941 gcgatgatgc tctggctttt ggtgactttg ccaacccagc gcttctcttc at
//
LOCUS       DAORHD010000095         5978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13429_length_5978_cov_3.299510, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000095 DAORHD010000000
VERSION     DAORHD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13429_length_5978_cov_3.299510"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="PKO06_03200"
     CDS             <1..1962
                     /locus_tag="PKO06_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNV68680.1"
                     /translation="IGVHALHQEVRQPIDQLKPFVGATVRVTGLFAGELRFRRQGGYR
                     CRWEDIRGTLDGQPFTLSRRFQVDIDASEPFPEPGQRYSATGTLHLDRNGTVRLTCAS
                     IVPAPEGVWFSRTIGRLQQWVRDGSRSLLSSRHQALNVGLLLGDTSQLSHQDRRLFRE
                     TGLTHLLAVSGQHVMVLALLLAAFLTWCGVPPLSRGLISIGFLTVFGFMAAGQPSVWR
                     AVAMYAVGLLIWNIEANPGPIRPVALTALLLLIWNPHWIHHIGFQLSFLAVLGIIIGR
                     PPLEQFLMRLHLPLLFARYLAVSIGANLATLPLAAYHFGTISLISFLINPLVVWSFGI
                     LLPLGIVLVCLGHLWFNGALLVAASLSLAFDAFLSVAEAASRLPGGLLFTGHLPGSLV
                     ALTYAALLYLLWETSLFHDRSTIASKTVTTIEASGISSSQPQTSAAARTNDSTQTIRV
                     SDSTKSSGELSASQEKYPPHRLSVAQLKSIDDRLASVPRRSLKTSSNLVAITFPVQKL
                     KVESQTIFHRLDDLTPEILRQEPLRAAQAISLCLTLVAVEILARLPQTAVQKATTWLN
                     DTVPPLQSRHLTTILLASRALAEPRAGLDPAVAPFATQAEELLRDGTTLLTTGPATDS
                     PAAPLPQQQLRTRVLEWCRLATHIDETSR"
     gene            complement(2039..3259)
                     /gene="clpX"
                     /locus_tag="PKO06_03205"
                     /pseudo
     CDS             complement(2039..3259)
                     /gene="clpX"
                     /locus_tag="PKO06_03205"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
     gene            complement(3268..3873)
                     /gene="clpP"
                     /locus_tag="PKO06_03210"
     CDS             complement(3268..3873)
                     /gene="clpP"
                     /locus_tag="PKO06_03210"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNV68681.1"
                     /translation="MTLVPIVVEQSSRGERAYDIYSRLLKDRIVFISGPIEDTMADLI
                     IAQLLFLESEDPEKDISLYINSPGGVVTSGLAIYDTMQYIKPDVSTICIGQAASMGAV
                     LLAAGAAGKRFAVPNARIMIHQPSGGAQGMASDIEIQTAEILRLKEKLNDVMAHHTGQ
                     PLKRIKKDMDRDFFMSSADAKEYGIIDDVIQRNKAPKVKAG"
     gene            complement(3848..5203)
                     /gene="tig"
                     /locus_tag="PKO06_03215"
     CDS             complement(3848..5203)
                     /gene="tig"
                     /locus_tag="PKO06_03215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNV68682.1"
                     /translation="MLKTQVQELEPNKVALSVEVGKEKVVAAYESFFLRAANSVKIPG
                     FRKGKVPRKIIENHLGKEAIREQVEEELVDEMYPVAVRDAKLHPVSRAKIEDVQLKEG
                     ESFAFKAIIEIRPKLPEFSYTGREVTAQRIKLEEAGVDKVLEKLREQFSRTVPVENGT
                     LDINDYFTVTCEVKVDGEVDKDLSEEKGYHRLIEANRLFLPLKGCKAGDQKTYQVPFT
                     SEGDKGSKYEGKTLEITATLENVSRPMKPELNDEFAKEVGEYQNLEALKAKIREDLEQ
                     QLLTDAEDRAYDAILKQVSDETTFAIPESMVQRTIDFFLHSIDRRWRQYNTSLEEVLK
                     RSNKDIKEYREGFREKAVYETRVMLITDSIAERQHIEVTDAEFMAEVERQAKEYGYPV
                     EKLLEMFKQSDGEDNVRHTLLNRKIREFLLKNNQIHYDMVSEADFNKGESDSDAGTHS
                     S"
     gene            complement(5256..5328)
                     /locus_tag="PKO06_03220"
     tRNA            complement(5256..5328)
                     /locus_tag="PKO06_03220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5293..5295),aa:Gly,seq:tcc)
     gene            complement(5342..5423)
                     /locus_tag="PKO06_03225"
     tRNA            complement(5342..5423)
                     /locus_tag="PKO06_03225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5387..5389),aa:Leu,seq:tag)
     gene            complement(5499..>5978)
                     /locus_tag="PKO06_03230"
     CDS             complement(5499..>5978)
                     /locus_tag="PKO06_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68683.1"
                     /translation="PLAAAARYWVGKKQATEKVNQERKVVKVDSYINKTLTVDRVVDN
                     PSTFIGQAVDWEGKAVCSTDSKGNTIYVVNSNPNVNSDNDLSQSFGVIFPKDLPNDPR
                     VSDAYSTIRVKGKIVRVEKVMNTWTSFLSRDRQPILEASEATFSRENYPDPLVIRFY"
BASE COUNT         1164 a         1756 c         1586 g         1472 t
ORIGIN      
        1 atcggcgtgc atgccctgca ccaggaagtt cgtcaaccga tcgatcagct caaacccttt
       61 gtcggcgcca ccgtgcgtgt caccgggctg tttgccgggg aactccgctt tcgccgtcag
      121 ggtggttatc gctgtcggtg ggaagacata cgaggaaccc tcgacgggca gcccttcact
      181 ctctctcgcc gtttccaggt cgatatcgat gcctccgagc ctttcccgga acccggccaa
      241 cggtacagcg ccaccggcac cctgcatctc gaccgaaatg gaacggttcg actcacctgc
      301 gcatcgatcg ttcccgcgcc tgaaggcgtc tggttttcac gcaccatcgg ccgcctgcag
      361 cagtgggtgc gcgacggcag tcgcagtctg ctgtcttcgc ggcaccaggc attgaatgtc
      421 ggcttactgc tgggtgacac cagccaactc agccaccagg accgacgctt gttccgggaa
      481 acggggctga cgcacctgct cgccgtctca gggcaacacg tcatggtgct cgcccttctg
      541 ctggcggcat tcctcacctg gtgtggagtt cctcccctct cacggggtct gatcagcatc
      601 ggatttctga ccgtcttcgg tttcatggca gccgggcaac cctcggtctg gcgcgccgta
      661 gcgatgtatg ccgtaggtct gctgatctgg aatatcgaag ccaaccccgg tcccattcgc
      721 ccggtggccc tgactgctct gttgctgctc atctggaatc cgcactggat ccatcacatt
      781 ggttttcagc tgagctttct tgccgttctg ggcatcatca tcggtcgccc gccactggaa
      841 caatttctga tgcgcctgca tctccctctt ctgttcgcgc ggtatctggc cgtgtcgatc
      901 ggtgcaaatc tggcaactct gcccctggct gcctatcatt tcggaaccat ttcactcatc
      961 tcgttcctca tcaatcccct ggtggtctgg tcattcggaa ttcttctgcc actcggcatc
     1021 gttctggtct gcctcggcca tctctggttc aacggagctc tgctggtagc ggcgtcactt
     1081 tcccttgcct tcgacgcttt tctgtccgta gccgaagccg cttcacgtct tccgggcggt
     1141 cttctcttta ccggtcatct tccgggttca ctggtcgctc tgacctacgc tgccctgctg
     1201 tatctgctct gggaaaccag cctttttcat gacagaagca cgattgcttc caagacagtg
     1261 acgacgattg aagcgtccgg aatcagctcc tctcaaccac agacaagtgc agcagcaagg
     1321 acaaacgatt cgactcagac gatccgtgtg tcggatagta caaaatcatc aggtgagcta
     1381 tctgcttctc aagagaagta tccacctcat cgtttatcag ttgcgcaact caaatctatc
     1441 gacgatcgtc tcgccagcgt gccgcgacga tcgttaaaaa catcatcaaa ccttgtcgcc
     1501 atcacttttc ccgttcagaa gctgaaggtt gaaagccaga cgatttttca ccgactggac
     1561 gatttgaccc ccgaaattct tcgtcaggag ccactgcggg cggctcaggc catctcactc
     1621 tgcctgactc ttgtcgctgt cgaaatcctc gcgcgcctgc cgcagaccgc ggtacagaaa
     1681 gcaacaacgt ggctcaacga cacggttcca ccactgcagt cccgacatct gaccacgatc
     1741 ctgctggcat ctcgcgccct ggcggaacct cgcgccggcc tcgatccagc cgttgcgccc
     1801 ttcgccacac aagccgagga actgctgcga gatggcacga ctcttctcac caccggcccc
     1861 gcgaccgact cccccgcggc gcccctcccc cagcagcaac tgcgaacacg cgtcctggag
     1921 tggtgtcgat tggcgacaca catcgacgaa accagccgct aaagcaaaac cgcgaagctg
     1981 aggggcttcg cggttttgcc tgttggtgtg atcaggcggt cttttcgtta ctctgcaccc
     2041 gatacacgag ctggcagggc tcggttttat cgatcgcccg ttcgtgtacg atcaccttgg
     2101 cgatttcctt gttcgaggga atctcataca tcagatcgag catcagctct tccagaatgc
     2161 gtctcaggcc acgagcacca gtactctggc gcagggcgcg tcgagcaatc gccttgagtg
     2221 cggccggcgt gacttcaagt tcgacgcctt ccatgcggaa gagacggtta tactgtttga
     2281 gaatcgcgct cttgggttcg gtcaaaatgc tgaccaggtc ttcttcaccc agcggctcaa
     2341 acgtcgtgaa gatgggcacg cgaccaatga attccgggat catgccaaac ttcagcagat
     2401 cttcgggcat cagcatcgac aggaatctgc caacctgttt ttccttcttg gccaccggcg
     2461 ttgagccaaa acccatcaac ttcacctggc tgcgattttc gatgatcttt tcgataccct
     2521 cgaacgcgcc gccgagaatg aagaggatgt ctttggtatt gatcgggatg taatcctgat
     2581 gcggatgctt gcgccccccc tgcggtggca catgggcgat cttgccctcg agcatcttca
     2641 aaagcgcctg ctgcacgcct tcaccggaca catcacgcgt tatcgacagg ttctcgctct
     2701 tgcgggcgat cttgtcgatc tcgtcgatgt agacaatgcc cttctcggcc ttctgcacat
     2761 cgtaatccgc attgcgcagc agattgagca gaatgctctc gacgtcatcg cctacgtacc
     2821 ccgcttcggt cagggtcgtg gcatcggcga tacagaatgg aacatgcaga atcttggcga
     2881 gcgtctgggc gatataggtc ttcccacagc ccgtcgggcc cagaaacagg atattcgatt
     2941 tctgaacttc aacatcgtct tttcggctct gctgcagcgc aatacgtttg tagtggttgt
     3001 acacggccac ggcaaccacc ttctttgcgc gctgctgacc aatgatgtac tggtcgagga
     3061 tcgagacgat ttccttgggt ttgagcagat tcttgttttc gacggtcacg tcgtcatcga
     3121 gctcttcaac gatgatctca ttgcagagat tgatgcactc gtcacagatg tagacttcgg
     3181 ggcctgcgat gagcttccga acctgatcct gaccctttcc gcagaaggaa caacggaact
     3241 ggggattctt ggaaagcatt gactctccta gccggctttg accttgggcg ccttgttgcg
     3301 ttgaatcacg tcgtcaatga tgccgtattc cttggcatcg gcggaagaca tgaaaaagtc
     3361 tcgatccatg tctttcttga tgcgcttcaa gggttggccg gtgtggtgag ccatgacgtc
     3421 attcagcttt tctttcaggc gcagaatttc ggcggtctga atctcgatat cagacgccat
     3481 gccctgcgcg ccgccggaag gctgatgaat catgatgcgg gcgttcggca cggcaaaacg
     3541 cttgccggct gctcccgctg ccagaagaac cgcgcccata ctggctgcct gaccgatgca
     3601 gatggtactg acgtcgggct tgatgtactg catcgtgtcg tagatggcca gtccgctggt
     3661 gacgactccg cccgggctgt tgatatacag cgaaatatct ttctcggggt cttcggattc
     3721 gagaaacagc agctgggcaa tgatcagatc ggccatggtg tcttcaatcg gaccgctgat
     3781 gaagacgatg cgatctttaa gcaggcgcga gtaaatgtca tacgcgcgct cgccgcggct
     3841 actttgctca actactatgg gtaccagcgt cactgtcgga ctcccccttg ttaaaatcgg
     3901 cttcactcac catatcgtaa tgaatctggt tgttctttaa caaaaattcg cgaattttgc
     3961 ggttcaacaa ggtatgtcga acattatctt cgccatcaga ctgtttgaac atctcgagca
     4021 gtttttcgac gggatacccg tattccttgg cctgacgctc gacttcagcc atgaactcgg
     4081 catcggtgac ttcgatatgc tggcgttcgg caatcgaatc ggtgatcaac atcacgcggg
     4141 tttcatagac cgccttctcg cggaacccct cgcgatattc cttgatatcc ttgtttgagc
     4201 gcttgagcac ttcttcgagc gaagtattgt actggcgcca gcggcgatcg atgctgtgca
     4261 ggaagaagtc gatcgtgcgc tggaccatgg attccggaat cgcgaaggtc gtctcatccg
     4321 atacctgctt cagaatcgca tcataggcgc ggtcttcggc atcggtgagc aactgctgtt
     4381 cgagatcttc acgaatcttg gctttcagcg cctcgagatt ctggtattcg ccgacttcct
     4441 tggcgaactc atcgttcagt tcgggcttca tcgggcgaga cacgttttcg agcgttgccg
     4501 tgatctcgag cgtcttgcct tcgtatttcg agcctttgtc tccttcagag gtgaatggca
     4561 cctgataggt cttctggtcg ccggctttgc agcccttcag cggcaggaac aggcggttcg
     4621 cttcgatcag gcggtggtaa cccttttctt cggaaaggtc tttgtcgact tcgccatcga
     4681 ccttcacttc gcaggtgacg gtgaaatagt cattgatatc aagcgtgccg ttttcgaccg
     4741 gaacggtgcg actgaactgc tcgcgaagct tctcgagaac cttatcaacg ccggcttcct
     4801 cgagcttgat gcgctgcgcg gtgacctcac ggccggtata ggaaaactca ggcagttttg
     4861 gtcggatttc gatgatggcc ttgaacgcga atgattcgcc ttctttcagc tgcacgtcct
     4921 cgatcttggc gcggctgacc gggtgcagtt tcgcatcacg aaccgcgacc gggtacatct
     4981 catcgacgag ttcctcttcg acctgctcgc gaatcgcctc tttgccgaga tggttctcga
     5041 tgatcttgcg gggaactttg cctttgcgga agccgggaat cttgacactg ttcgcggcac
     5101 gcagaaagaa gctctcatag gccgcgacaa ccttttcctt cccgacctca acactgaggg
     5161 cgactttgtt gggttcgagt tcctggacct gggtcttgag catggttgcg cgactccttc
     5221 agattgggaa tgaatgtttt gcggagaaaa aaaagagcgg gagacgggat tcgaacccgc
     5281 gacctcctgc ttggaaggca ggagctctac caactgagct actcccgcat cacatttcct
     5341 gtgcgaaggg cgggactcga acccgcacgg tgtataccac gagatcctaa gtctcgcgcg
     5401 tctgccagtt tcgccaccct cgcgtgtgga tctgaaatga acgcaagaga ggataccaga
     5461 ttctgtacct tctggcaagt cccttctctg agggaaattc agtagaaacg aatgaccagc
     5521 ggatcggggt agttttcgcg ggaaaaggtc gcttcgctgg cttcgagaat cggctgtcgg
     5581 tcacggctca gaaaggaggt ccaggtgttc atgactttct cgacgcgcac gatcttgccc
     5641 ttcacgcgaa tggtcgaata ggcatcggaa acccgaggat cattcggcag gtctttggga
     5701 aaaatcaccc cgaatgactg ggacaaatca ttgtccgagt tcacgttcgg gttcgagttc
     5761 accacataaa tggtgttgcc cttgctgtcg gtgctgcaga cagctttgcc ctcccagtcg
     5821 accgcttgcc caatgaacgt cgatgggtta tcgaccacgc gatcaacggt gagcgtcttg
     5881 ttgatgtacg agtcgacttt aacgactttc cgctcctgat ttactttttc ggttgcctgt
     5941 ttcttaccga cccagtagcg ggccgccgcc gcgagcgg
//
LOCUS       DAORHD010000096         5954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13494_length_5954_cov_3.541278, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000096 DAORHD010000000
VERSION     DAORHD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13494_length_5954_cov_3.541278"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PKO06_03235"
     CDS             complement(<1..327)
                     /locus_tag="PKO06_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV68684.1"
                     /translation="MEVAKQDAVLGIARHWLDRGVDGFRLDTANLYFHSRDLQDNPPL
                     PEGQRGDSPVLMQQHLHNADQPEAPAFFERLRALMDSYGAQRADGSGARMAVAEIGGA
                     EPWPTMV"
     gene            349..1863
                     /locus_tag="PKO06_03240"
     CDS             349..1863
                     /locus_tag="PKO06_03240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68685.1"
                     /translation="MMIDNLARSGIRKFSPHRLPLEEQGRVKPIDVLMESLCSAEELG
                     WSQINGRPGRLLSLGVSGGRTLVYWNVFPALASGPAFMFGGQEPEHLVRNHITELAQA
                     EQTRGEINGVFVAHLDVVPERSAAWSREMRTLAQLAERTRRVQQRQLTVDGRTVWAVA
                     MIDSHAGRYALIHAYDATQDMKSLGRMRFGICLSIIPVLLMAWGAGLLLGDLLLHPIS
                     DLERGIEAIRSRDTSHRIPVRRDDEFGSLARLFNQSLLELKELELAREVQGSLLPREL
                     PRINGWSIGGVNVTASDLGGDYYDLLATHEGKEWVMCVGDVTGHGASAALAMAMAKAG
                     LTYRLNQGEVALKPLLDSLNLIFYNELRSSRKFMTMVLNRFCPATGDIEFENAGHNYP
                     LIYRRATAVSEFVQMVGSPLGVKRKGVRDSAKHRLDPGDAVLCYTDGYPECSLSSGEF
                     MGDEAMKQRFEELCRTGHDAASIVQHLLQDLQQRRSPGPLQDDVTLVVIKREEV"
     gene            1866..2384
                     /locus_tag="PKO06_03245"
     CDS             1866..2384
                     /locus_tag="PKO06_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68686.1"
                     /translation="MSRSLGKRWFFVLLCLCGAFLISGCAERVEMDGAVVFCMPAWTW
                     LTAAALGLVFAVLAVRAASQGWRLWLVTALCWFLFVPGLYGDRVILTKDSIESLRGFP
                     FSSNSVAVRFDDVKQLIISTYKDSKGRLKTRLVFEGKGSQRRMLPAGDLVRAAMPELL
                     QIFDQRHIPIIR"
     gene            2397..3719
                     /locus_tag="PKO06_03250"
     CDS             2397..3719
                     /locus_tag="PKO06_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HNV68687.1"
                     /translation="MKNTLIRWGIALIVGVLVAGAAVQAQARHELGDEALLDLVQRQT
                     LRYFWEHAHPTSFMAPERNTTPNCVTTGGTGFGLMAWVVGCERGWFERREVLRRSHVL
                     VDFLARADRWHGAWPHWLDGTTGKVIPFWKKDDGADLVETSYLVMGLLTLDAYFSRSN
                     LDERSLREKIRKLWREVEWTWFTRGENVLYWHWSPNFGWEMNHRIRGWNECLITYLLA
                     ASSPTFPISPNAYHQGWAGSEHFKNGNQYYRLPPLPLGEKLGGPLFFSHYSFLGIDPR
                     GLRDAYADYGEQVRIHTLTNYYYCVENPKRYRGYSSRCWGLTASDNDKGYSAHSPTND
                     LGIITPTAALSSMPFTPLQSMRALRHFLQVYEGRLWGKCGFYDAFSPHRDWIAESYLA
                     IDQGPIIVMLENHRTSLLWRLFGEHADVRRGLKRLGFVRMSSRQGGGL"
     gene            3716..>5954
                     /gene="bglX"
                     /locus_tag="PKO06_03255"
     CDS             3716..>5954
                     /gene="bglX"
                     /locus_tag="PKO06_03255"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HNV68688.1"
                     /translation="MKCVVSLATWLRCRVVVLAVVLFCQPCWCGAIEEVRACLDLLGR
                     MTLEEKIGQLTLYTSTWAQTGPTLDANYLHYIRQGRCGAVFNAMTASYTRSLQRIAVE
                     QSRLKIPLLFGYDVIHGHRTIFPIPLAEACSWNLEAIERSARIAAIEAAAEGIHWTFA
                     PMVDVARDPRWGRVSEGAGEDVWLGCQIAHARVRGFQGGDLSAPDTILACAKHFAAYG
                     AAQAGRDYHSVDLSERTLEEIYLPPFEACVKAGARTFMTAFNDLNGIPCTGNRALLTD
                     LLRSRWGFTGVVVTDYTSINELIPHGFATDLRQAAQLAIEAGVDMDMQGGAFLTHLAE
                     LVRVGIVSEQQIDQAVLRVLQVKQQLGLFDDPYRYCDEERERRVVFCEEHLRAAEDLA
                     RQSLVLLQNESRTLPLQKTLTRVAVIGPLAESRVDLLGNWHGAGDENKVETILERVRE
                     KLGAGCQVRFVKGCDVTGTRRLDVSGVLQAVADAEAIILVLGEHEHMSGEAASRADLD
                     LPGRQNELAELVGITARRAGKPVAAVLVNGRPLVLTRLSRAVPAILEAWFPGTRGARA
                     IADVVFGDAAPVGKLVMTFPRCVGQVPIFYGMKNTGRPFDAANKYTSKYLDVENTPLF
                     PFGHGLSYTTFSYAHLKLDQTQISSDDTLRVTVDVTNSGDRAGTDVVQLYLRDHVGSV
                     TRPVKELKGFARVALAPGETRAIEFRLSADDLAFLRADMTRGVEPGTCSVMVGGSSET
                     LLAADF"
BASE COUNT         1091 a         1635 c         1936 g         1292 t
ORIGIN      
        1 caccatggtg ggccagggct cggcgccgcc gatctcggcc accgccatgc gcgcgccgct
       61 gccgtctgcg cgctgcgcac cgtaactgtc catcaaggcg cgcagccgct cgaagaaggc
      121 cggcgcctcg ggctggtcgg cgttgtgcag gtgctgctgc atcagcaccg gcgaatcgcc
      181 gcgctggccc tcgggcagcg gcgggttgtc ctgcaagtcg cggctgtgaa agtagaggtt
      241 ggcggtatcg aggcggaagc cgtccacccc gcggtcgagc cagtgccggg cgatgcccag
      301 caccgcgtcc tgtttcgcaa cttccatgac gatcgcaatg atctgttgat gatgatcgac
      361 aatctcgccc gctccggtat tcgcaagttt tcgccgcatc gactgccgct ggaagagcag
      421 gggcgggtca agccaatcga tgtgttgatg gaaagcctgt gctctgcaga agaattgggg
      481 tggtcgcaga tcaatgggcg gccggggcga ttgttgagtc tcggcgtcag tggcggacga
      541 acgctcgtct actggaatgt gtttccggcg ttggcctctg gaccggcgtt catgttcgga
      601 ggccaggaac cggagcatct tgtgcgcaac cacattaccg aactggcgca ggccgagcag
      661 acccgcggcg aaatcaacgg ggtgttcgtt gcccatctcg atgtggttcc cgagcgatca
      721 gcggcctgga gccgggagat gcgcaccctg gcgcaactcg ccgaacgtac ccgccgcgtg
      781 cagcagcggc agctgacggt cgatggacgc accgtctggg ccgtcgccat gatcgactcg
      841 catgccggaa gatatgccct gattcacgca tacgatgcga cgcaagatat gaagtcactc
      901 ggacgcatgc gattcgggat ctgtctgagc attattcccg tgctgctgat ggcctggggg
      961 gcgggtttgc tgcttggtga tctgctgctg cacccgatca gcgatctgga acgagggatc
     1021 gaggccattc gttcgcgcga cacgtcgcat cggattccgg tgcgtcgcga cgacgagttc
     1081 ggttcgctgg cgcgcctgtt caaccagtca ttgctggaac tgaaagaact cgaactggct
     1141 cgtgaagtgc agggaagcct tctgccacgt gaactgcccc gaatcaacgg gtggtcgatt
     1201 ggcggtgtga atgtgaccgc gagcgatctg ggcggtgatt actacgatct tctggccacg
     1261 catgaaggga aagagtgggt gatgtgcgtc ggtgacgtga ccggtcacgg agcttcggct
     1321 gctctggcga tggcgatggc gaaggcgggg ctgacctatc ggctgaacca gggcgaagtg
     1381 gccttgaaac ctctgctcga ttcgttgaat ctgattttct acaacgagct gcgcagctcg
     1441 cgaaagttca tgacgatggt gctgaatcgc ttttgtccgg ccacgggaga catcgagttc
     1501 gagaacgccg ggcacaacta tcctctgatc tatcgtcggg cgaccgcggt cagtgagttc
     1561 gtacagatgg tgggttcacc actgggcgtg aagcgcaaag gggtgcgcga ttcggcgaag
     1621 catcgacttg acccgggaga cgccgttcta tgctataccg acgggtatcc tgaatgctcg
     1681 ctgtcgagcg gagaattcat gggggatgaa gcgatgaaac agcgattcga agagttgtgc
     1741 cgcacggggc atgatgcggc cagcatcgtg caacatctgt tacaggatct gcagcagcgg
     1801 cggagccccg gaccgctgca ggatgacgtc accctggtcg tgatcaagcg cgaggaggtc
     1861 tgaagatgag ccggtcgctg ggcaaaagat ggtttttcgt gctgctgtgt ctctgcgggg
     1921 cgttcctgat cagcggttgt gcagaacgtg tcgaaatgga cggggccgtc gtcttttgca
     1981 tgccggcctg gacatggctg accgctgcgg ctctcgggct ggtctttgcc gtactggctg
     2041 ttcgggccgc gagtcagggg tggcgcttgt ggttggtgac ggcgttgtgc tggtttctct
     2101 ttgtgcccgg cctgtatggc gatcgggtga tcctgacgaa agactcgatt gagtcacttc
     2161 ggggatttcc gttttcgagc aattcggtcg cggttcgttt cgacgatgtg aaacagttga
     2221 ttatctcaac gtacaaagac tccaagggga gactcaagac gcgactggtg tttgaaggca
     2281 agggttcgca gcggcgaatg ttgcctgcag gcgatctcgt acgggcggca atgcctgagt
     2341 tgctgcagat tttcgatcag cgacacatac cgatcatcag ataacgggag gggacgatga
     2401 agaacacgct catccgctgg ggtattgctc tgattgtagg agttctggtc gcgggtgccg
     2461 cggttcaggc tcaggcccgt cacgaactgg gtgatgaagc cctgctcgat ctcgtgcaga
     2521 gacaaacgct gcgttacttc tgggaacatg cgcatccgac ttcttttatg gctcccgagc
     2581 gcaatacgac gccaaactgc gtgacgacag gtggtactgg tttcggcctg atggcctggg
     2641 tcgtgggctg tgaacgcggc tggttcgagc gtcgcgaggt gctgcgccgg tcacatgtgt
     2701 tggtcgactt tctcgcccgt gccgatcgct ggcacggagc ctggccgcac tggctcgacg
     2761 gcacgacggg caaggtgatt ccgttctgga agaaggatga tggtgccgac ctggtcgaaa
     2821 cgtcctatct ggtcatgggc ctgctgacgc tcgatgccta cttctcacgc tcgaatctgg
     2881 acgaacggtc gctgcgagaa aagattcgga agctgtggcg cgaggtcgaa tggacctggt
     2941 tcacccgtgg agaaaacgtg ctctactggc actggtcccc gaacttcggc tgggaaatga
     3001 accaccgcat tcgcggctgg aacgagtgtc tgattaccta tctgctggcg gcatcgtcgc
     3061 caacgtttcc catttcaccg aacgcctatc accaaggctg ggccggaagc gaacatttca
     3121 agaatggaaa ccagtactat cgtctgccac cacttcccct gggtgaaaaa ctgggtggtc
     3181 ctctgttctt ctcgcactac tcatttcttg gtattgaccc gcgcggcctg cgtgatgcat
     3241 atgccgacta tggtgagcag gtgcggattc atacgttgac caactattac tactgcgtcg
     3301 agaatccgaa gcggtatcgc gggtactcgt cgcggtgctg ggggttgacc gccagcgaca
     3361 acgacaaagg ctactcggcg cattctccca ccaacgatct tggcatcatc accccgacgg
     3421 cggcgctgtc atcgatgccg tttaccccgc tgcagtccat gcgggccctg cgccactttc
     3481 tgcaggtcta cgaaggcaga ctctggggca agtgtgggtt ctacgatgcg ttcagtcctc
     3541 atcgtgactg gatcgctgag agctatctgg cgatcgatca ggggccgatc atcgtgatgc
     3601 tcgaaaacca tcgtacgagt ttgttatggc ggttgttcgg tgaacatgct gatgtgcgac
     3661 gtggtctcaa acgtctggga tttgttcgaa tgtcatcgcg tcaaggaggt gggctatgaa
     3721 gtgcgtggtg agtctggcca catggttgcg ctgccgtgta gtcgtgttgg ctgtggtgct
     3781 gttctgccag ccctgttggt gcggcgctat cgaagaggtg cgtgcctgcc tcgatctgct
     3841 tggccgcatg accctcgaag aaaagattgg tcagttgact ctctatacga gcacctgggc
     3901 gcagacgggg ccgacgctcg atgcgaacta tctgcactac atcaggcagg ggcgctgcgg
     3961 ggccgtgttc aacgccatga cggccagcta tacccgcagt ctgcagcgca ttgccgttga
     4021 acagtcgcga ctgaagatcc ctctgctgtt cggctacgac gtcattcacg gtcatcgcac
     4081 gatctttccc atccctctgg cggaagcctg ttcctggaat ctggaagcga tcgaacgctc
     4141 cgctcgcatc gccgcgatcg aggcggcggc cgagggcatt cactggacct ttgccccgat
     4201 ggtcgatgtg gcgcgtgacc cccgctgggg gcgggtgtcg gagggcgcgg gcgaagatgt
     4261 ctggctcggc tgtcagattg cgcatgcgcg cgtgcgcggg tttcaggggg gtgacctgtc
     4321 agcgcccgat accattctgg cctgtgccaa gcattttgcc gcatacggtg ccgctcaggc
     4381 cgggcgagat tatcactcgg tcgatctgtc agaacgaact ctcgaagaga tctatctgcc
     4441 gccgtttgaa gcctgtgtca aggcgggagc ccgaaccttc atgacggcgt tcaatgattt
     4501 gaacggcatt ccctgtaccg gaaaccgtgc cttgctgacc gatctgctgc gatcgcgctg
     4561 ggggttcacg ggggtggtcg tcacggacta cacctcgatc aacgaactga ttccgcacgg
     4621 tttcgcaacc gatctgcgtc aggccgcgca gctggcgatc gaggccggag tcgacatgga
     4681 catgcagggc ggagcgtttc tgacgcacct ggccgaactg gtgcgtgtcg gcatcgtctc
     4741 ggaacagcag atcgaccagg ctgttctacg tgtgcttcag gtgaaacaac aactcggact
     4801 ttttgatgat ccgtatcgct actgtgacga agaacgggaa cgccgcgtgg tgttctgcga
     4861 ggaacacctg cgggcggccg aagatctggc gcgtcagtca ttggtactgc tccagaacga
     4921 gagtcggact ctgcctctgc agaagacgct gacacgtgta gcggtgattg gtccgctggc
     4981 tgaatctcgc gtcgatctgc tggggaactg gcacggagcc ggcgatgaaa acaaggtcga
     5041 aacaattctg gaacgtgtgc gcgagaagct gggcgctggt tgccaggtca ggtttgtgaa
     5101 agggtgtgac gtcaccggaa ccagacgtct cgacgtgtct ggtgttctgc aggcggtcgc
     5161 agacgcggaa gcgatcattc tggtgctggg cgaacatgag cacatgagcg gtgaagccgc
     5221 atcgcgtgcc gatcttgatc tgcctggtcg tcagaacgaa ctggctgaac ttgtcggcat
     5281 caccgcccgc cgggctggaa aaccggttgc cgccgtgtta gtcaacggcc gacctctggt
     5341 gctgacccgc ctcagtcggg ctgtacccgc tattctcgaa gcctggttcc cggggactcg
     5401 tggtgcccgg gcgatcgctg atgttgtctt cggcgacgcc gctcccgtgg gcaagctcgt
     5461 gatgaccttt ccgcgctgtg tgggtcaggt gccgattttc tatgggatga agaataccgg
     5521 gcgtccgttt gatgccgcca acaagtacac gagcaagtat ctcgacgttg agaatacgcc
     5581 gttgttcccc ttcggccatg gtctgtctta taccacattc tcttatgccc atctgaagct
     5641 ggatcaaacg cagatcagct cggacgatac tctgcgggtg accgtcgacg tgacaaattc
     5701 cggagaccgg gcgggaaccg atgtcgtgca gttgtacctg cgcgaccatg tcggatcggt
     5761 gactcgacct gtgaaagaac tcaaggggtt cgcgcgagtg gcgctggcac ccggagaaac
     5821 ccgcgcgatc gagtttcgac tgtccgctga cgatctggcg ttcctgcggg ctgacatgac
     5881 gcggggagtc gagccgggaa cctgctcagt catggttggc ggcagttcgg agacgcttct
     5941 tgccgctgac ttct
//
LOCUS       DAORHD010000097         5943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13527_length_5943_cov_4.066067, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000097 DAORHD010000000
VERSION     DAORHD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13527_length_5943_cov_4.066067"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="PKO06_03260"
     CDS             <1..1121
                     /locus_tag="PKO06_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68689.1"
                     /translation="DPVPELWKSLFSTFQTVSDKPVFFQQPTDRFIRETFVGVVGGVR
                     QSLVILLLLSILLVIREHLSSSNLFKGLVFCVIAGDLLWNAWGFFQFTFPYPVSGFGP
                     EIREYLHRQPGPFRIVTPYCLAQNDALIERYETVSGYDTLVWSAYSEFINVSQGRARQ
                     EPTLSFEALHPSPLLDLLNPRFWIYPRAAATAPAPLELRLTTSDWTLYENPRAFPRFY
                     AVSSARVLPDRASVLHQLTTTDLARTVLLETDRVCPSGSEEKGSSPLQVSILDWQPAR
                     QSLQVTLASSAYFICGDVNFPGWQATVDGVATPVMTGNGLFRTLWLEAGSHRIEFRYA
                     PTSLRRGLCVSVISFLLWLMAVSRLLWLRCRHTPPVDH"
     gene            complement(1147..2211)
                     /locus_tag="PKO06_03265"
     CDS             complement(1147..2211)
                     /locus_tag="PKO06_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNV68690.1"
                     /translation="MSRGKKLFGLLVSLLFLWLALRKVDWQQIPAILSQVQIGYLLIL
                     SLTLVGEVISRTYRWQVILQDRQLPFGLLFNGLSLGYFFNNLFPARAGEFARAIYISR
                     RTSVRASEIFGSIILERFLDGIVVMGFLLITLTCFDVSPTIRAGGISAFIFYLSVFLF
                     MSLFQFRRAWVARLLEFVLRPFPEGWRERVMSVQTALSQGFSLLRQPWNLLHAIFLSF
                     IAWTISVLTLWCCLQAVNIIVGWRETILLITVLSLGAMIPSSPGMIGLYEYLCVLVLS
                     EMLHYPHEVAITFGILMHILGMLFYAIIGTVILFRENLALKEFEKADDESDPLEICRE
                     PEQIAETAQPRVSADSPPNA"
     gene            2334..3275
                     /locus_tag="PKO06_03270"
     CDS             2334..3275
                     /locus_tag="PKO06_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68691.1"
                     /translation="MLYVEVMLIGLAAFGLCFFLGKKLGERKFRRVNEEMKALELSFN
                     QLLEQMELTSAHNLKVLEAKTEDLRQLLPIVDKKVLQAQDLTQQLDEYASRKPAVAAP
                     SSLDPAREIKLRRELQESIATVQNRLSSLEQRLQGLEHRATEWDARVEASSHATNRSG
                     ARMVPVADLPFSATGSEKVIDIKSRELRREREGVGQMTSEMTPTLESIVRQAEKQLKE
                     VQQLREMTAVVAGDTRRLEPVAAPEAEFSPLPSELPEELTGEDVPAPGTLFYDVLTLS
                     QQGVTIPQIARKMNIGNGEVELIMKLYGSRFNLRKVM"
     gene            3282..4385
                     /locus_tag="PKO06_03275"
     CDS             3282..4385
                     /locus_tag="PKO06_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNV68692.1"
                     /translation="MFIRELPATAKNVLILLGGIVSLWLTWQLSDVIAPFLVSLVLAY
                     ILNPLIHAARYLYLPRPWAVLALFVLGLGLSVVILIPFVLNIVSEGHDLASRLQTVDV
                     QQMTNQYRLQGQELYDRYSQIPWVREYLGVAWNNDRVRDLAAKAVVMAKDVVMGVIRK
                     VFSWITAAFSGVMGLFLIPLLTFYMLLDLELLFAQGLLLVPPVYRVSTERILAEIDSL
                     LSSYVRGQIVSCLIFGSLATLGLWLSGLPFAVLLGPMAGLANLIPYLGGLVTVILATL
                     VALVQTGVSWAFVILMIKVAVTLSIVQAIDGFLVQPKIVGDSVGLHPLMVILALIVGG
                     SLFGLLGMLLSVPVTCVFKVLSRELYHELYDAA"
     gene            4375..5310
                     /locus_tag="PKO06_03280"
     CDS             4375..5310
                     /locus_tag="PKO06_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV68693.1"
                     /translation="MPPETIPAAIRVQGLNKWFGRKQVLEHIDFTVPAGAVAGFLGPN
                     GAGKTTTLRILLGLARAYNGTMEALGLPMPSCRTAAMEQIGTLVEQPAFLDSMSGAKN
                     LWWFGNLYQPVTAERVREVLELVGLGEAASKPFGVYSTGMKQRLGVAAALLHRPKLLI
                     LDEPTNGMDPQGRAQMRDIFKQIHAETGVTIFLSSHLLDEIQRLCDYVVIIDRGKTVR
                     EGWVKDILRGDIECWEVRFPAPDTDRVRGLLKNLGCVQAVNDVPRGLELTLTPGSSAE
                     VVRLLVESKVAVQAVIPRESSLEETFLALTETKGL"
     gene            5313..>5943
                     /locus_tag="PKO06_03285"
     CDS             5313..>5943
                     /locus_tag="PKO06_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV68694.1"
                     /translation="MRRFWILLRFELVKVLARKKALLFLLALNVLPIMSGLMAMLVYI
                     KYQGWGLGEVQFSFVVEGVKKLLAGHVKLFALISPFFLALVIGDSFSGESGKGLMKTL
                     LLTPVPRWQVIIAKTMAVMLFLITALAVGGVFLQFSLFVARAVTSSPSAEGLSQMVGN
                     SPAVAEAVNQALKDRSTSLQLVGAMPALRLLLITFLANLSVVGFFILFSL"
BASE COUNT         1205 a         1586 c         1734 g         1418 t
ORIGIN      
        1 ccgacccggt tcctgaactg tggaagagtc tgttctcaac gttccagact gtctctgaca
       61 aaccggtgtt ctttcaacag ccgaccgacc gttttattcg tgaaacgttt gtcggggttg
      121 tcggcggcgt gcgtcaatcc ctggtcattc tgctgctcct gtccattctg ctcgtcattc
      181 gagagcacct ctcctcgtcg aatctcttca aggggctcgt gttttgcgtg attgccggtg
      241 acctgctctg gaatgcctgg ggcttctttc agttcacctt tccctaccca gtgtcaggat
      301 tcgggcctga aatcagggaa tatcttcacc gacaacctgg accatttcgc attgtcaccc
      361 cctactgtct ggcacagaac gatgcactga tcgaacgcta tgaaacggtc tcgggatatg
      421 acacgctggt ctggtcagct tactctgagt tcatcaatgt cagccaggga cgtgcccgcc
      481 aggaaccgac cctgtcattt gaggccctgc atccctctcc gctcctcgat ctgctcaatc
      541 cccgtttctg gatctacccc cgcgctgcgg ccactgctcc cgccccgctg gaactccgac
      601 tgacgacttc tgactggacg ctgtatgaaa atccccgggc ttttccacgc ttctacgctg
      661 tctcatcagc acgggttctt ccagatcgcg catctgtgtt gcatcagctg acaacgactg
      721 acctggccag aaccgtgttg ctcgaaactg atcgcgtgtg tccgagtggc agtgaagaaa
      781 aaggttcttc accgctgcag gtcagcatac ttgattggca gccggcaagg cagagtctgc
      841 aggtgactct ggcgtcttcg gcatacttca tttgcggcga tgtcaatttt ccggggtggc
      901 aggccacggt cgatggggtg gcaactccgg tgatgaccgg caacggactc tttcgtaccc
      961 tgtggttgga ggcaggttca catcgaatcg aatttcgata tgctcctact tctcttcgtc
     1021 gcgggctctg cgtctctgtg atttctttcc tgctgtggct gatggcagtg agtcgcctgt
     1081 tgtggctgag atgcaggcac acgccgccgg ttgatcatta gccagccgat gcgtggggca
     1141 gaagaatcag gcgttgggcg gagagtcggc ggaaacgcgt ggttgagccg tttccgcgat
     1201 ttgttccggt tctcggcaga tttcgagtgg atcgctttca tcatcggctt tctcgaactc
     1261 tttgagtgcc agattttcgc gaaagagaat gacggttccg atgatcgcgt aaaaaagcat
     1321 cccgaggatg tgcatgagaa tgccgaaagt gatggcgact tcatgcggat agtgcaacat
     1381 ctcggaaagt accagcacgc aaagatactc atacagcccg atcattcccg ggcttgacgg
     1441 aatcatggcg ccgagtgaga ggaccgtgat cagcagaatg gtctcccgcc aaccgacgat
     1501 gatatttacc gcttgcaggc agcaccagag agtcaagacg gaaatggtcc aggcgatgaa
     1561 cgagagaaag atcgcgtgca gaagattcca gggttgacgt aacagagaga acccctgcga
     1621 cagcgctgtc tgaacactca tcactcgctc gcgccatccc tctggaaacg gcctcaggac
     1681 gaactcgagt agacgggcca cccaggctct tctgaattga aagagcgaca tgaacagaaa
     1741 gactgacaag tagaagatga aggcggaaat gccgcccgcg cgaatggtcg ggctgacatc
     1801 gaaacaggtc agagtgatca gtagaaaacc catgacgaca atgccatcaa gaaagcgttc
     1861 gaggatgatc gagccgaaga tttctgaggc tcgaaccgag gttcgacgcg aaatgtagat
     1921 ggctcgtgcg aactcgccgg cccgcgccgg aaacagattg ttgaaaaagt atccgagcga
     1981 cagaccgttg aacagaaggc cgaatggcaa ctgtcggtct tgcagaatca cctgccagcg
     2041 ataggtgcgg ctgatcactt cacccacgag ggtaagcgag agaatgagga gatagccgat
     2101 ctgcacctgg ctgagaatgg ccggaatctg ctgccaatca acttttcgca gtgccagcca
     2161 gaggaacagc aggctgacga gtagcccgaa aagctttttc ccgcgtgaca tggggcaatc
     2221 gtatcacacc cgttcggcga cgagcgaaaa aaatcgtcaa gttcctggcg gcgtggtctt
     2281 gtggaaccag ggtccggtga atataatggg tcatcttctt ccaggggcgc cacatgctgt
     2341 acgtcgaggt catgctgatc ggtctggcag ccttcggcct gtgctttttc ctcggcaaga
     2401 aactgggaga acgcaagttc agacgggtca atgaagagat gaaagcgctc gagttgtcgt
     2461 tcaaccagct gctcgagcag atggaactga cctcagccca caacctgaaa gtcctggaag
     2521 caaagaccga agatctgcgc caattgctgc caatcgtaga taaaaaagtt cttcaagccc
     2581 aggatctcac gcagcaattg gatgagtatg ccagtcgcaa acccgctgtc gccgccccgt
     2641 cgtctctcga tcccgcccga gaaatcaaat tgcggcgtga gctgcaggaa tcgattgcga
     2701 cagtgcaaaa tcgtctttca agtcttgaac agcgcctgca ggggctcgaa catcgggcga
     2761 ccgaatggga cgcgcgcgtg gaagcctcgt ctcacgcgac gaaccggtcg ggtgctcgaa
     2821 tggtaccggt tgccgatctg cccttcagtg cgactggctc agagaaagtc atcgacatca
     2881 agtcgcgtga gctgcgtcga gaacgtgagg gggtcgggca gatgacgtcc gagatgacgc
     2941 cgacactcga atcgattgtt cggcaggctg aaaaacagct caaagaagtg cagcagttgc
     3001 gagaaatgac cgccgtggtt gccggtgata cccgacgtct tgaaccggtt gcggctcctg
     3061 aagccgagtt ttcgcctctg ccgagtgagt tacccgaaga actgaccggc gaagatgttc
     3121 cggcaccggg tacgcttttc tacgatgtcc tgactttgtc gcagcagggc gtcacgattc
     3181 cacaaattgc ccgcaagatg aacattggaa acggcgaggt cgagttgatc atgaaactgt
     3241 acggttcccg gttcaatcta cgcaaggtca tgtaggcccc cgtgtttatc cgtgaactgc
     3301 cggcgactgc caagaatgtc ctgattctgc tgggcggaat cgtttcgctg tggctgacgt
     3361 ggcagctgtc tgatgtcatt gctccgtttc tggtttcact ggttctcgcc tatattctga
     3421 atccgctgat tcatgctgcc cgatatcttt atctgccgcg accctgggct gtgcttgcgt
     3481 tgttcgttct cggactcgga ctgtccgtcg tgattctcat tccctttgtg ctcaacatcg
     3541 tctctgaagg acatgatctt gcctctcgcc tgcaaacggt cgatgttcag cagatgacga
     3601 accagtaccg acttcagggc caggaactgt atgaccgcta ctcgcagatc ccctgggtga
     3661 gagaatatct gggtgtcgcc tggaacaatg atcgggtgcg tgatctggcg gcaaaggcgg
     3721 tggtcatggc caaagatgtg gtcatgggag tcatacgaaa agtgttttcc tggatcactg
     3781 ccgcgttttc cggagtgatg ggactgttcc tgattccact gctgaccttc tacatgctgc
     3841 tcgatctcga actgctgttc gctcaggggt tgttgctggt gcccccggtc tatcgtgtgt
     3901 cgaccgaacg cattctcgcc gaaatcgatt cgctgctcag cagttacgtt cggggccaga
     3961 ttgtttcatg cctgatcttc ggttctttgg cgactttggg gttgtggctt tccggtctgc
     4021 catttgccgt gctgctcgga ccgatggccg gcctggctaa cctcatcccg tatctcggtg
     4081 gtctggtaac cgtgatcctc gctacgctgg tagccctggt tcaaaccggg gtgtcctggg
     4141 cgttcgtgat actgatgatc aaggtcgcgg tcaccctgtc gattgtgcag gccatcgacg
     4201 ggttcctggt acaacccaag attgtcggcg acagcgtcgg cctgcatcca ttgatggtga
     4261 ttctggcatt gattgtcggt ggcagtctgt ttggcctgct cggcatgctg ctgagcgttc
     4321 ctgtcacctg cgtgttcaaa gtcctcagtc gagagttgta tcacgagttg tacgatgccg
     4381 cctgaaacaa tcccggctgc catccgcgtt cagggtttga acaaatggtt tgggcgtaag
     4441 caggtgttgg aacacatcga ctttaccgta ccggccggcg cggtggcggg gtttcttggg
     4501 cccaatggtg ccggcaagac gacgacgctg cgcatactgc tcggtctcgc gcgtgcctac
     4561 aacggaacca tggaagcact cggattgccg atgccgtcat gtcgaacggc agccatggaa
     4621 cagatcggca ctctggtcga acagcccgcg ttcctcgaca gcatgagtgg agcgaagaat
     4681 ctgtggtggt ttggtaattt gtaccagccg gtaacggcgg aacgcgttcg ggaagtgctc
     4741 gaactggttg ggcttggcga agcggcgtcg aaaccgttcg gcgtctacag taccggtatg
     4801 aagcagcgac tgggggtggc cgcggcgttg ttgcatcgcc ccaagctgct gattctcgat
     4861 gagcccacca acggcatgga tccccaggga agagcgcaga tgcgcgatat cttcaaacag
     4921 attcacgcgg agaccggagt gaccattttt ttgtcgtcac atctgctcga tgagatccag
     4981 cgcctctgtg attatgtggt catcatcgat cggggaaaga cggtgcgtga aggctgggtg
     5041 aaagacattc tgcgtggcga catcgagtgt tgggaagtcc gttttcccgc ccccgataca
     5101 gaccgtgttc gcggtcttct caagaacctg ggttgtgttc aggcggtgaa cgatgtgccg
     5161 cgtggtcttg aactgacact gactcctggt tcttctgccg aagtggtgcg cctcctggtt
     5221 gaatcgaaag ttgcggtaca ggccgtcatt ccacgcgaat cctcactcga agagacgttc
     5281 ctggcattga ccgagacgaa agggctttga cgatgcgtcg attctggatt ctgctgcgtt
     5341 tcgaactggt caaagtgctg gcccgtaaaa aggcactgct gtttctgctg gccttgaacg
     5401 tactgccgat catgtcgggg ctcatggcga tgctggtcta tatcaaatac caggggtggg
     5461 gtctgggaga agtccagttt tctttcgtcg tcgaaggggt gaagaagctt ttggcagggc
     5521 atgtcaaact gtttgcgctc atctctccgt tctttctggc tctggtgatc ggtgatagtt
     5581 tttcgggcga gtccgggaaa ggcttgatga aaacgttgtt gctgaccccg gtgccccgat
     5641 ggcaggtgat cattgccaag acgatggccg tcatgctgtt tctcattacc gcgctggccg
     5701 tgggtggggt ctttctgcag ttcagtctgt ttgtcgcccg ggccgtgacc agtagcccgt
     5761 cggccgaagg gctgtctcag atggtcggga actcgccggc tgttgctgaa gccgtgaacc
     5821 aggctctgaa agatcgttcc acctccctgc agctggttgg ggccatgccg gctctacgcc
     5881 tgttgctgat cacctttctg gccaatcttt cggtggtcgg gttcttcatt ctgttttccc
     5941 tgt
//
LOCUS       DAORHD010000098         5910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13643_length_5910_cov_4.493424, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000098 DAORHD010000000
VERSION     DAORHD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13643_length_5910_cov_4.493424"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKO06_03290"
     CDS             <1..689
                     /locus_tag="PKO06_03290"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68695.1"
                     /translation="YLHTEGERRFIRQLFGRYVNDKVIDAIMLLPRDDVLKGHRQHVC
                     VLFIDIRGFTTYSESRPPNQIVDFLNRYFESLTEIIMRHNGVVDKFLGDGLMAFFNAP
                     IPRPSFLEDAVAAVLEIRETLAARKIRTTDDAFDLKVGMALHTGDAVIGNIGSERKME
                     FTAIGDTVNTTSRLESLNKDYGTDIIVSDSVVIGTNKTYEWKFLADHQIRGKERPVPI
                     YTLVGKKLYG"
     gene            713..1873
                     /locus_tag="PKO06_03295"
     CDS             713..1873
                     /locus_tag="PKO06_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68696.1"
                     /translation="MKRLIQTVLGGLWLLCAVTPVFCAVEPVFMVNDVTGNVAASAAD
                     STWIPLLGETLSEGTRLELKSASDTLDLVHLKLQKEIRIQGPKVLTIDKQGLQGVSEK
                     DYGQSVGGIPSDLAMNADGLNQVGAVRDHAQVGAGGGKSKGIQASRVLKPQSVDGMKG
                     AGEESAQQALALEEKLLEQEREKKRQELEGDKNESSPSESTHQEMTTGEPSAASSDNF
                     TSMQNTESVPKKLVREQRDAGASAMAAPAASAPVMNTISLEVAVPEKALESALYSPDT
                     GVTVNVNDQDYKASRTDASIKGWVVLRVPGVREEEQLIMSLVDKDLRNLQFIKLHLTS
                     TTGVLDALTLESKGCHEQAAAVWFKLLQQGKVIRKVAEAHLKRLASKIEKTR"
     gene            1891..2481
                     /locus_tag="PKO06_03300"
     CDS             1891..2481
                     /locus_tag="PKO06_03300"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68697.1"
                     /translation="MSPMEPKDFPSLAVNWRKLRGVVLFADVCGFFDLARKSPLEDLG
                     TVISQLYGFWGGEIRRQRGEVVSYIGDSVLALFTADACGTMDPEWCATLAAFHIVKGM
                     KKIRPDLEITVGLNSGEILEGRWDESGRQMQTILGDVVNRAAMLVGGPARPKGIHASQ
                     PVVDVLGPRVAREKVTLRVPNNGEEWTIWRLNSLVL"
     gene            complement(2547..3974)
                     /locus_tag="PKO06_03305"
     CDS             complement(2547..3974)
                     /locus_tag="PKO06_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV68698.1"
                     /translation="MDSTPGIKMKFCESLDRFVAIRAFTPEQRQQLFKNTKITDKKSY
                     KRLIINAAVVNYLDEIAPLVFGNNEYPLLGEIIEQELYNLCVKVNQALDIKEVTIQVD
                     DPSGQNIPMLGQEAEKGKSQRFMEIENHLKKRIIGQDEAVTAVAQAIRKAKVGLKNPK
                     RPVGSFIFVGQTGVGKTELAKALCEFLTGQETNLVRIDCSEYAMSHEYAKLIGAPPGY
                     IGHNEGGFLTESVKAKPNTVVVFDEIEKAHEKVHNLLLQLLDEGILTDSKGETVSFRE
                     CCIILTTNIGVSDITTEESRPGFRPSAGKGGEEEKLKLENLSHDQKSKITRKSLEKKF
                     PPEFLNRIDEIVVFRALTKDDNLQILDIMLTEVADRLKNLGMGIEFTPKLRQFLVDNG
                     TDLKYGARPLRRTIHRYIENPLAEMILKGEGAKRGDLMTSDLSKDTAGEDCATFDVTG
                     TFEAPPPTPETETAPPPPAPGNKQK"
     gene            4084..5517
                     /locus_tag="PKO06_03310"
     CDS             4084..5517
                     /locus_tag="PKO06_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68699.1"
                     /translation="MTIKEAEEHFHRGIHYFRGGFFPAALQEFQAVARIDPQFGNIQH
                     FLTVTRKKTEEVAGQLSAFLHEAFDDQAHQLSEQLVIEDSPHFGREIERLLRREEYQA
                     ALTKLADAEAFVSESRPFLLLKANVFKRLGRFADAEQILLRAHTLFPEDAEIINNLGN
                     VYLARNHFNEARDRFEAALKLNPDDPRALNNLGTLYMQIHNLDRAQSVFERLVREHPN
                     WQVARRNLEGLLRRKEELEKEIFRLRQELIAHPTYIDIGMNLGKNLFFRGFFTEARLT
                     LESVLRQNPSMHAAQFYLGSICEIEGDFQKALERYYAMCVARQQDDSPAFINSQRLRE
                     EGYLEEALAEVKKVAVLELDLASGHIKLGIRYFEDGQWDNAQEHFDEAVKLKPKYPDA
                     YYWRALTRLRTGKKASAEKDLRKAIELNPRFADAHYQLGMLSRDKAPRKARFHLQAAI
                     SMGVRAQFAEIAERLLKTPDGDDEEED"
     gene            5535..>5910
                     /locus_tag="PKO06_03315"
     CDS             5535..>5910
                     /locus_tag="PKO06_03315"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68700.1"
                     /translation="MEEVLFQENFPAFPTRPEDLQKLSVVLRSQRVLSKEDWDKLKSE
                     NSDPQKLILAIALRNDVKPKNLAEAEASILNVPFIDLETMNIPNEVMKLFKPEQMVEH
                     RVIPIKITGQELLVGMLSPADEE"
BASE COUNT         1319 a         1549 c         1708 g         1334 t
ORIGIN      
        1 tgtacctgca caccgaaggg gaacggcgct ttattcgcca gctgttcggt cggtatgtca
       61 atgacaaggt catcgatgcc atcatgctgc tgccacgaga cgacgttctc aaaggacatc
      121 gccagcacgt gtgcgtgttg tttatcgata ttcgtggttt taccacctat tctgaaagtc
      181 ggcctccaaa ccagatcgtc gatttcctca accggtattt tgaatcgttg accgaaatca
      241 tcatgcgtca taatggcgtg gtcgacaaat ttctgggcga tgggttgatg gccttcttca
      301 atgcgccgat tcctcgcccc agctttctgg aagatgccgt ggccgccgta ctcgagattc
      361 gtgaaacgtt ggctgcccgg aaaatccgga cgactgatga tgcgtttgat ctgaaggttg
      421 gcatggcact gcatacggga gatgccgtta tcgggaacat tggttcggaa cgcaaaatgg
      481 agtttaccgc gatcggtgat acggtcaata ccacttcgag gctggaaagc ttgaacaagg
      541 actatggaac cgatatcatt gtgagcgatt cggtggtgat tggtaccaac aagacctacg
      601 aatggaaatt tctggcagat catcagattc ggggcaagga aagaccggtc ccaatctata
      661 cgctggtcgg aaagaagttg tacggatgat ggtgaagttc aggaggatta ctatgaagcg
      721 tctgattcag accgtgctcg gaggcctgtg gcttctttgt gccgttacgc ccgtgttttg
      781 tgccgtggag ccggttttca tggtcaacga tgttaccggg aatgtggcgg caagtgctgc
      841 cgattctacc tggattccac tcctgggcga aaccctcagt gagggcaccc gcctcgaact
      901 gaagagcgcc agtgatacgc tcgatctagt gcatctcaaa ctgcagaaag aaatccgtat
      961 ccaggggccc aaagtcctga cgatcgacaa acaggggtta cagggtgtct cggaaaagga
     1021 ctatggccag tcggtaggtg ggattccttc tgatctggcg atgaatgctg acggtctgaa
     1081 tcaggtcggt gcggttcgcg atcatgctca ggttggtgcc ggtggaggca agtcgaaggg
     1141 aatccaggcc tcccgggttc tgaaacctca gtcagttgac gggatgaaag gcgccggcga
     1201 agagtctgct cagcaggctc tcgcgttgga agaaaagctg ctggagcagg aaagggagaa
     1261 gaaacgccaa gaactggaag gcgacaagaa cgaatcgtcc ccatcggagt ccacacacca
     1321 ggaaatgaca acgggagaac cgagtgccgc ttcgtcagat aacttcacgt cgatgcagaa
     1381 tacggagtct gtgcccaaaa aacttgtcag agaacaacgc gatgctggcg caagtgcaat
     1441 ggctgcgccg gccgcttcgg cgcccgtcat gaacaccata tcgcttgagg ttgccgtgcc
     1501 ggagaaagcg ctggaaagtg ccctgtactc tcctgacacg ggcgtgaccg tgaatgtgaa
     1561 tgatcaggat tacaaggctt ctcgaaccga tgcctcgatc aaggggtggg tggttctgcg
     1621 ggtgcccggt gttcgcgaag aagagcagct gatcatgagt cttgttgaca aagacctgcg
     1681 taacctgcag ttcatcaaac tgcacctgac gagcacaacc ggcgttctgg atgctctgac
     1741 tctcgaatcc aagggttgcc acgaacaggc ggcggctgtc tggttcaaac tgttacagca
     1801 gggaaaagtg attcgcaagg ttgcagaagc tcatctgaag cgccttgcgt ccaaaatcga
     1861 aaagaccaga tagggtttgg aacattcccc atgagtccta tggaaccgaa agactttcca
     1921 tcgctggcgg tgaactggcg caagctgcgc ggcgtggtgc tgttcgcgga tgtctgcggt
     1981 ttcttcgatc tggctcgcaa gagcccactc gaagaccttg gcacggtcat ttcgcaattg
     2041 tacgggttct ggggcggtga gattcgccgt cagcgcggag aagtcgtgtc atacatcggt
     2101 gactcggttc tggcgctctt tacggctgat gcctgcggca ccatggaccc ggaatggtgc
     2161 gcgactctgg ctgcctttca catcgtgaag ggcatgaaaa agattcgccc ggatctggaa
     2221 attaccgtgg gcctgaattc cggtgagatt ctcgagggac gctgggacga atccggccgg
     2281 cagatgcaga cgattctcgg cgacgtcgtg aatcgagcgg ccatgctggt gggcgggcct
     2341 gcccgcccga aaggcattca cgcgtcacag ccagtggtcg atgtcctggg tccgagagtc
     2401 gcacgtgaga aagtgacatt gcgtgtgcca aacaatgggg aagagtggac aatctggcgt
     2461 ttgaattcgc tggttctctg aactgaagca aaaaacaaca cccccgcact tgtactctgg
     2521 tgcgggggtg tcttcttgcg tggcttttac ttctgtttat tgccaggcgc gggtggcggc
     2581 ggcgcggtct cggtctccgg agtggggggt ggagcttcga aggtgccggt cacgtcaaaa
     2641 gtggcacaat cttcgcccgc agtatccttg ctcaaatcgc tggtcatcag atcgccgcgt
     2701 ttggcccctt cacctttgag aatcatttcc gccagcgggt tttcgatgta ccggtgaatg
     2761 gtgcggcgca acggccgagc gccatacttc agatcggtgc cgttatccac gagaaactgc
     2821 cgcagtttcg gggtgaactc gatgcccatg cccagattct tgagtcggtc ggcaacctcg
     2881 gtcaacatga tgtcgagaat ctgcaggttg tcatccttgg tcagggcgcg aaacacgacg
     2941 atctcgtcga tgcggttgag gaactcgggc gggaacttct tctcgagcga cttgcgcgtg
     3001 atcttggatt tctggtcatg cgacaggttc tcgagtttca gcttctcctc ttcgccacct
     3061 tttccggcgc tgggccggaa gccggggcgg gactcttccg tcgtgatatc ggagaccccg
     3121 atgttggtcg tcaggatgat gcagcactcg cggaacgaaa cggtctcacc cttgctgtcc
     3181 gtgagaatgc cttcgtcgag cagctgcagc aacagattat gcactttctc atgggccttt
     3241 tcgatctcat cgaacacgac gacggtattg ggtttcgcct tcaccgactc ggtgaggaac
     3301 ccgccttcgt tgtggccgat gtatccaggg ggagcgccga tgagcttggc atattcatgg
     3361 ctcatggcat actcggaaca atcgatgcgt accagattgg tttcctggcc ggtcagaaac
     3421 tcacagagtg ccttggcgag ctcggtcttg ccgacgccgg tctgcccgac gaagatgaaa
     3481 ctgccgacgg gtcgcttcgg gtttttcagg ccgaccttgg ccttgcgaat tgcctgagcc
     3541 acggctgtga cggcttcatc ctggccgatg atacgcttct tgagatgatt ctcgatctcc
     3601 atgaagcgct gcgacttgcc cttctcagcc tcctggccga gcatgggaat attctgcccg
     3661 cttggatcgt cgacctgaat ggttacttct ttgatgtcga gcgcctgatt tactttcacg
     3721 cacaggttgt acagttcctg ctcgatgatt tcacccagaa gcggatattc attgttcccg
     3781 aataccaggg gagcaatctc gtccaggtag ttgaccacgg cggcattaat gatcagccgt
     3841 ttgtaggact ttttgtccgt gatcttggtg ttcttgaaaa gctgttgccg ctgctctggc
     3901 gtgaatgcac gaatcgcgac gaaccgatcg agcgattcgc agaacttcat tttgattccg
     3961 ggggtggaat ccatgactac cctccctggg ggtgcgttag tttccgctga caagcgaggt
     4021 tcaatcaggt atagggtatt gtatcacttg cctgtacgat aggcaacacc ccagaggaat
     4081 cgagtgacaa tcaaggaagc cgaagaacac ttccatcgag gtatacacta ctttcgcggt
     4141 ggtttttttc cggccgccct gcaggaattc caggccgtcg cccgcattga cccgcagttc
     4201 ggcaatattc agcattttct caccgtcacc cgaaagaaga ccgaggaagt ggccggacag
     4261 ttgagcgctt ttctgcacga agcgtttgac gatcaggcac atcaactgtc tgagcagctg
     4321 gtcattgaag attcccccca tttcggccgt gagatcgagc ggctgctgcg ccgcgaagag
     4381 tatcaggcag ccctgaccaa gcttgctgat gccgaagcct tcgtttcgga atcgcggccc
     4441 tttctgctgt tgaaggcgaa tgtcttcaag cggctcggtc ggtttgccga tgccgagcag
     4501 attctgttgc gcgctcacac gctgtttccc gaagatgccg aaatcatcaa caatctcggc
     4561 aatgtctatc tggctcgaaa tcacttcaat gaagcgcgtg accggtttga ggcggcgttg
     4621 aagctgaacc cggacgaccc gcgagccctc aacaatctgg gtaccctcta catgcagatt
     4681 cacaacctcg atcgcgcaca atcggtattc gaacgattgg tgcgggaaca cccgaattgg
     4741 caggtggccc gtcgcaatct cgaggggttg ctgcgccgca aagaagaact cgaaaaagag
     4801 atctttcgcc tgcggcagga gctgattgct cacccaacct acatcgatat cggcatgaat
     4861 ctggggaaga acctgttttt ccgtgggttt ttcaccgagg cgcgtctgac actcgaaagt
     4921 gtcttgcgtc agaacccctc gatgcatgcg gcgcagtttt atctggggag tatctgcgag
     4981 atcgaaggcg atttccagaa agcccttgaa cgctactatg ccatgtgtgt cgcccggcaa
     5041 caggatgatt cgccggcatt catcaactcg cagagactgc gagaagaggg atatctcgaa
     5101 gaggctctcg ccgaagtgaa gaaggtcgcc gtgctcgaac ttgacctggc gtcaggtcat
     5161 atcaagctcg ggatcaggta tttcgaagac ggacaatggg acaatgcgca ggaacacttc
     5221 gatgaagcgg tcaaactcaa accgaagtat cctgatgcct actactggag agccctgacc
     5281 cgcttgcgta ccggcaagaa agcctcagcc gaaaaagatc ttcgcaaagc gatcgaattg
     5341 aacccgagat ttgcggatgc ccattaccag ctgggaatgc tgtcgcgcga caaggccccc
     5401 aggaaagcgc ggttccatct gcaggccgcg atcagcatgg gggtgcgcgc gcagtttgcc
     5461 gagattgctg aacgcctgct gaaaacccct gacggcgacg acgaagaaga agactgaggg
     5521 agcggccgaa aaccatggaa gaagtcctgt ttcaagaaaa ctttcccgcg tttccgacgc
     5581 gtcctgaaga tctgcaaaag ttgtccgtcg ttctgcgctc tcagcgggtt ctctccaaag
     5641 aagactggga caaactcaaa tcggaaaact ccgatccgca gaaactcata cttgccattg
     5701 ctctgcgcaa cgacgtcaaa ccgaagaacc tcgccgaagc cgaagcgtcg attctgaacg
     5761 tgccgttcat cgaccttgaa acgatgaaca ttcccaacga agtcatgaag ctgttcaaac
     5821 cggaacagat ggtcgaacat cgggtcattc ccatcaagat caccggccag gagcttctgg
     5881 tcggcatgct gtcaccggct gacgaagagg
//
LOCUS       DAORHD010000099         5895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13692_length_5895_cov_4.390925, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000099 DAORHD010000000
VERSION     DAORHD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13692_length_5895_cov_4.390925"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKO06_03320"
     CDS             complement(<1..693)
                     /locus_tag="PKO06_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68701.1"
                     /translation="MRKQRFIISIVFLALCLFLAAPVFAADEPSGLKLDLKGESSPRL
                     PRSLGEFNVSLDSTLAPQRTMADTIRPTRDLLDLPRAKVVSPELEAQNTDDTEPLVTT
                     PTPVSPNSQSLSSTFTFRPGYLQTNLTDRFTSRSTRTVRPQGSRLSLTGITQQEPLPL
                     VTLPAPTPTPVFANRVRPRGALPSAAPGSPILLPSPAVVPSVVDHYLLEATYNFNPAV
                     QGKVSYQRSQFDP"
     gene            complement(788..1681)
                     /gene="rapZ"
                     /locus_tag="PKO06_03325"
     CDS             complement(788..1681)
                     /gene="rapZ"
                     /locus_tag="PKO06_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNV68702.1"
                     /translation="MNGEGHLQPQDRVNLTIVSGLSGAGKSQAMHYFEDAGYFCIDNL
                     PPALLPKFVELCKDTHGKIKSLAVGIDTRGGEFFQNLFDALATVKELGFTYRILFLDA
                     TDEVLVRRFSETRRRHPLSSGGRILDDIQYERRTLSELRESADVVINTSDLTARELYE
                     EIRRFIEHDSTSRKFPVVIVSFGFKHGVPLDCDLMFDVRFLPNPYYLPEMKNLTGIDE
                     QVYHYVTQSELGAEFGAKLLSFIEYLIPHFIQEGKSRLQIAIGCTGGRHRSVAFAEFL
                     YREMQKDHILLSRRHRDLDLK"
     gene            complement(1690..2145)
                     /locus_tag="PKO06_03330"
     CDS             complement(1690..2145)
                     /locus_tag="PKO06_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HNV68703.1"
                     /translation="MIDLKVSGTSTERPRTNTTPKPTSTPTEGPAPAFTASLKAARRT
                     LLEGNLSELLGKIRALGKKFLQSPSNDSLEEYRDGVGQFLERVSKELFSLKQELGLEK
                     DGQQKAYQLVETVNRELETLTRETLRDEQALRLLGSLDEIRGLVLDLLS"
     gene            complement(2149..4062)
                     /locus_tag="PKO06_03335"
     CDS             complement(2149..4062)
                     /locus_tag="PKO06_03335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV68704.1"
                     /translation="MNSGRMPLVWRLDSLIALLFIVTYFLAGALGQPLPVVPFFACGA
                     LVISCAHLLVHVKIIKPLAYFAKVADDIADGDTRSLNPEILASNSAEDIQASFGKIAG
                     SLIESRDAVENYMQTISDINKQLASKVDSLSLLYSISKAMGGSLSIDSLVRTFLTVIN
                     DHMHVNGAMVLLYNDRNDMVTVKDLLGFPPELFARFRFFSDHKIIMNVFAAEEGIWVP
                     DEASVNLMKAEFETDAVHELKLFTPMRIKDHFVGIIVLGSKKDGSDFSESDCKLVQAF
                     TGLATTAVNNAMLYERSESTKNELDRKVFNLMTLQQAGKVLSSTLNLDELIRIAIDMF
                     LETVWSNKGVLMLQGEDSLDMIVRAAKGIPPEELKALEKDAAEVWAMTTLRKEKRPIL
                     AQELATKSFFQSYTAINRALAFAVYIPLLKEGEIYGVVKIGAKINGEPFTENDLEFFS
                     TLSSQATIAFENARLYSLAITDSITKLYVHRYFQLRLDEEVKRSRRYNSTLTLLMCDI
                     DHFKEINDKYGHQQGDIILKEVSLILRKNIRSTDIAARYGGEEFVIILPETTQSDARI
                     VAERIRRDVSKFDFPALAPEQPPVKCTISIGVAGFPLNANDKDELIQKADASMYQAKG
                     AGRNRVMLYGIDC"
     gene            complement(4059..4748)
                     /gene="tmk"
                     /locus_tag="PKO06_03340"
     CDS             complement(4059..4748)
                     /gene="tmk"
                     /locus_tag="PKO06_03340"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006640370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNV68705.1"
                     /translation="MKRSSRSHHAPKPATSRAHGWFFTFEGPEGSGKTTQIRKLSEYL
                     TERGHTVLITREPGGTPCGDAIRSLFLQTPGGHLEAETEMFLMLAQRTEHWRKVIKPA
                     RDAGNIVLCDRYIDSSLAYQGYGRGLGADRILSIHQEFLGDCLPDLTIVFSIDPRDGL
                     KRAQAGGKTRLDRMESEQIGFHHRVHAGYLELSHRFPERFAVVDASGQIDATYAQILA
                     VVEAMHPELLT"
     gene            complement(4748..5071)
                     /locus_tag="PKO06_03345"
     CDS             complement(4748..5071)
                     /locus_tag="PKO06_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV68706.1"
                     /translation="MKIECKVDEKNRQRDYHLDGELDVHQAKLLKERLVEDLEEIQEG
                     SWVIRLHMENVQYLDSSGLGMLVYLKKEIKLRHGKLHIDGLKDAVYNVFKLTRLDEFF
                     ELPKP"
     gene            complement(5068..5538)
                     /locus_tag="PKO06_03350"
     CDS             complement(5068..5538)
                     /locus_tag="PKO06_03350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68707.1"
                     /translation="MSAESSFVPPAQAVTVQLQLTGNPKCLGLVRRVLSASIGQFRLP
                     SGVLNDLKLAVTEACTNVIRHSFKYDFHRQFSLTLEFTSSVLRIRIIYEDRTFEPEKI
                     PLPDLTSAPEGGLGVFIIRKIMDNVRYATNRETGEITLTMEKFIAQEPSTGGQP"
     gene            complement(5552..>5895)
                     /locus_tag="PKO06_03355"
     CDS             complement(5552..>5895)
                     /locus_tag="PKO06_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68708.1"
                     /translation="RWGLGDASIEFSKGAVRQKHLVSHVKTCEKVCREHPDMAIAYAE
                     LGRCHLLFDDLAQTDKALTQLETSFFLDPQSMTTAMLLAKAYRALGKPARVDAIRQNV
                     ILSEPEIQSKL"
BASE COUNT         1251 a         1544 c         1793 g         1307 t
ORIGIN      
        1 gggatcgaac tgtgaacgct gatacgaaac ctttccctga accgcgggat tgaagttata
       61 cgtggcttcg agaaggtaat ggtcgacaac cgaaggaacg accgcaggag aaggcagcag
      121 aatcggcgac cccggcgcgg ccgagggaag ggccccgcgc gggcgaaccc ggttggcaaa
      181 caccggggtt ggggttggtg ccgggagagt gacaagtggc agtggctcct gctgggtgat
      241 cccggtgagg ctgagtcgcg agccttgtgg ccgcaccgtt cgtgtcgagc ggctggtgaa
      301 gcggtctgtc aggttggtct gcagataccc gggacggaag gtgaatgtcg atgacaggct
      361 ctgggaattg ggtgataccg gcgtcggggt tgtcaccaag ggctctgtgt catcggtatt
      421 ctgtgcctcg agttcagggg aaaccacttt tgctcgtggg agatcgagaa gatcgcgggt
      481 aggacgaata gtgtcggcca tggtgcgctg aggtgccagc gtgctgtcga gtgacacgtt
      541 gaattcgccg agtgaacgag ggagtcgtgg actggattcg cccttgagat cgagcttcag
      601 gccagaaggc tcatcggcag cgaaaaccgg cgctgccaga aagaggcaga gcgccagaaa
      661 aacgatactg atgatgaagc gctgtttccg cataagtccc tcagacaaac acaaaatata
      721 ctctccaccg ctcccgaaag caagggattt gtgaagtttg acaccacagg accgacagaa
      781 aaccgggtta cttgagatcc agatcgcggt gtcggcgtga caggagaatg tggtctttct
      841 gcatttcacg gtagagaaac tcggcaaagg cgacggagcg atgccggccg ccggtacagc
      901 cgatggcgat ctgcaggcgt gatttccctt cctgaatgaa atgaggaatc agatactcga
      961 tgaagctcag aagtttcgcg ccgaactcgg cgccgagttc cgattgggtc acataatgat
     1021 agacctgctc gtcaattccg gtcagattct tcatttccgg taggtagtac ggattcggca
     1081 ggaacctcac atcgaacatc agatcgcaat ccagtggaac tccgtgtttg aacccaaagc
     1141 tgacgatgac gaccggaaac ttgcgactgg tcgagtcgtg ctcgataaac cggcggatct
     1201 cttcatagag ttcccgggcg gtgaggtcag aggtgttgat gacgacatcg gctgattcgc
     1261 gcaactcaga gagcgttcgt cgttcatact gaatgtcgtc gagaatgcgg ccgcccgagc
     1321 tgagtgggtg gcgccgcctc gtttcggaaa aacgtctgac cagaacctca tcggtggcgt
     1381 cgagaaagag aatgcgatag gtgaagccga gttccttgac agtggccagg gcatcgaaca
     1441 gattctgaaa gaactcgccc ccacgggtgt caatgccgac ggcaagggat ttgatcttgc
     1501 cgtgcgtgtc cttgcagagt tcgacgaact tcggcagcag ggccgggggc aggttgtcga
     1561 tgcagaaata gccggcgtct tcgaagtaat gcatggcctg actttttccc gcacccgaga
     1621 gcccggaaac aatggtcaga ttgactcgat cctgtggttg caggtgacct tcgccgttca
     1681 tgtgatgcct caggaaagaa gatcgagaac gagaccgcga atctcgtcca gactgccgag
     1741 caggcgcagc gcctgctcat cacgcaacgt ttcacgggtg agcgtttcca gttcacggtt
     1801 cacggtttcg accagctggt acgctttctg ttgtccgtct ttttccagtc ccagttcctg
     1861 tttcagcgag aacagttctt tcgagacccg ctcgagaaac tgaccgacgc catcgcggta
     1921 ctcttccagc gagtcgttgg atgggctttg caggaacttt ttgcccagag cgcgaatctt
     1981 gccgagcagc tcgctgaggt tcccttcgag aagcgtacgc cgggctgcct tcagcgatgc
     2041 ggtgaaggcg ggggcagggc cttcggttgg cgtggaagtc ggcttggggg tcgtgttggt
     2101 gcgcggtcgt tcagtgctgg tgccggaaac tttcaggtcg atcatggttc aacaatcgat
     2161 gccgtacagc atgacgcgat tacgccctgc gcccttggcc tgatacatcg aggcatcggc
     2221 cttctgaatg agctcgtctt tgtcattcgc gttcagcggg aagcccgcga cgccgatcga
     2281 aatggtgcac ttgaccggag gctgttcggg agccagggcc gggaagtcga acttcgaaac
     2341 gtcgcgtctg atgcgttcgg caacgatgcg ggcatcggac tgcgtggtct cgggcagaat
     2401 gatgacgaac tcttcgccgc cgtatcgggc cgcaatgtcg gtcgatcgga tgttcttgcg
     2461 gagaatcaga ctgacttcct tgaggatgat gtccccctgc tgatgaccat acttgtcgtt
     2521 gatttccttg aagtgatcaa tgtcgcacat caacagcgtg agcgtggaat tgtaccggcg
     2581 actgcgcttg acttcttcgt ccaggcgcag ttggaagtac cgatgaacat acagtttggt
     2641 gattgagtcg gtgatcgcca gagagtacag gcgcgcattc tcgaaagcga tggtggcctg
     2701 cgagcttaag gtcgagaaga actcgagatc gttttcggta aacggctcgc cgttgatttt
     2761 cgcgccgatc ttcaccaccc cgtagatttc cccttccttg aggagcggaa tgtagacggc
     2821 gaaagcgagt gcccggttaa tggcggtgta tgactggaag aacgacttcg tggcgagttc
     2881 ctgagccagg atcggccgtt tttccttgcg cagggtggtc atcgcccaga cctcggcggc
     2941 atccttttcg agtgctttga gttcttcggg cggtatgccc ttggcggcac gaacgatcat
     3001 gtcgagagag tcttcaccct gcaacatcag gacgcctttg ttcgaccaga ccgtctcgag
     3061 gaacatgtcg atggcgatgc ggatcagttc gtccaggttg agtgtcgagc tcaagacctt
     3121 ccctgcctgc tgcagggtca tgagattgaa aaccttgcgg tcgagttcgt tcttcgtcga
     3181 ctcggagcgc tcatacaaca tggcgttgtt gaccgcggtg gtggccagtc cggtgaaggc
     3241 ctgcaccagc ttgcagtcag actccgaaaa gtcgctgcca tccttcttcg accccaaaac
     3301 gatgatgccg acgaaatggt ccttgatgcg catcggggtg aacagcttca gttcatgcac
     3361 ggcatcggtt tcgaattcgg ctttcatcaa attcaccgac gcttcgtccg gaacccagat
     3421 gccttcttca gcggcgaaca cgttcatgat gattttgtgg tccgagaaga agcgaaaacg
     3481 cgcgaacagc tcagggggaa agccgagcag gtctttgacc gtgaccatgt cgttgcggtc
     3541 gttgtagagc agaaccatgg ccccattgac atgcatgtga tcgttgatga cggtcaaaaa
     3601 agtgcgtacc aggctgtcga tgctgagact gccacccatg gccttgctga tggaatagag
     3661 aagcgagaga gagtcgactt tgctggccag ttgcttgttg atgtcgctga tcgtctgcat
     3721 gtagttctca acggcatctc gcgactcgat caaactgccg gcgatttttc cgaaactggc
     3781 ttgaatgtct tcggcgctgt tgctggccag gatttccggg ttgagagaac gggtgtcgcc
     3841 gtcggcgata tcgtcagcga ccttggcaaa atacgccagt ggtttgatga tcttgacatg
     3901 aaccagcagg tgcgcacaac tgatcaccag agcaccgcag gcgaagaatg gcacgacggg
     3961 aagtggttgt ccgagagcgc cggcaagaaa ataggtgacg atgaacagca gggcgatcag
     4021 gctgtccagt cgccacacca gcggcattcg gcctgaattc atgtcagcag ctccggatgc
     4081 atcgcttcaa cgacggcaag gatctgagca taggtggcgt cgatctgtcc cgaggcatcg
     4141 acgacggcaa accgctcggg gaaacgatgc gacagttcga gatatcccgc atgcacgcga
     4201 tgatgaaacc cgatctgttc tgattccatg cggtcgagcc gggtttttcc gcctgcctgg
     4261 gcccgcttga ggccgtcacg aggatcgatc gaaaagacga tggtcaggtc aggaaggcag
     4321 tcgccgagaa actcctggtg aatcgagaga atgcgatcgg ctcccagtcc gcgcccatac
     4381 ccctgatacg cgaggctcga gtcgatatac cgatcgcaca agacgatatt cccggcatct
     4441 cgtgcgggtt tgatgacttt gcgccagtgc tcggtgcgct gcgccagcat caggaacatc
     4501 tccgtttccg cttccaggtg cccgcccgga gtctgcagga acagcgaacg aatcgcatca
     4561 ccgcagggag tgccgccggg ttcgcgggtg atgagcaccg tgtgtccccg ctcggtgagg
     4621 tattcgctga gcttgcgaat ctgcgtggtt ttcccgctgc cctcaggacc ttcgaaggta
     4681 aaaaaccagc catgtgcgcg gcttgtagcc ggcttgggcg cgtggtggga tcgagaagat
     4741 ctcttcatca gggttttggc agttcgaaga attcgtcgag acgcgtcagc ttgaagacgt
     4801 tgtagaccgc atccttcagc ccgtcgatgt gcagcttccc atgtcgcagc ttgatctctt
     4861 tcttgagata cacgagcatt cccagccccg aactgtcgag atattgcacg ttttccatgt
     4921 gcagtcgaat cacccacgac ccttcctgaa tctcttccag gtcttcgacg aggcgttcct
     4981 tcagcaactt ggcctgatgc acgtcaagct caccatccag gtggtaatcc cgttggcggt
     5041 ttttctcatc caccttgcat tcgattttca cggttggcct cccgtcgagg gttcctgggc
     5101 aatgaatttt tccatggtca gtgtgatttc cccggtctcg cgattggtag catagcgtac
     5161 gttatccatg attttccgaa taataaaaac ccccagcccc ccttcagggg ctgaagtcag
     5221 gtctgggagc ggaatcttct caggttcgaa ggtccgatcc tcatagatga ttctaatgcg
     5281 aagtacgctg gaagtaaact ctaaagtcaa actgaactga cgatgaaagt cgtacttgaa
     5341 cgaatggcga atgacattcg tgcaggcttc cgtgacggcc agtttcagat cgttgagcac
     5401 gcccgagggc aaccggaact gtccgataga ggcgctcagg acgcgtcgca ccaggcccag
     5461 acatttcgga ttgccggtca gctgtaactg aaccgtgacg gcctgggcag ggggaacgaa
     5521 agacgattcg gcggacatgg tgtcgaaggc ctcagagctt ggattgaatc tcaggttcgg
     5581 atagtatcac gttctgacgt atggcgtcga cgcgcgcagg cttccccagt gctcgatacg
     5641 ctttggcgag cagcatggcc gtagtcatgg actgagggtc gaggaaaaag gaggtttcga
     5701 gttgggtcag agctttgtcg gtctgagcca gatcgtcgaa gagcaggtgg caacgcccga
     5761 gttccgcgta cgcgatggcc atatcgggat gttctcggca gactttttcg caggtcttga
     5821 cgtggctcac caggtgtttc tggcgcaccg cccccttgga aaactcaatc gacgcatcgc
     5881 cgagccccca acggg
//
LOCUS       DAORHD010000100         5895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13693_length_5895_cov_3.780651, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000100 DAORHD010000000
VERSION     DAORHD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13693_length_5895_cov_3.780651"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1311
                     /locus_tag="PKO06_03360"
     CDS             70..1311
                     /locus_tag="PKO06_03360"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNV68709.1"
                     /translation="MEWIKDRKELSESARVPVKSWCAEPEPEALKQAANLAAHPVVEH
                     HVALMPDCHVGYGMPIGGVIGCREAVIPNAVGVDIGCGMCAVKTSATVEQMRDLAFIR
                     AILEDVKKLVPVGEGNSHKRQQEWDGFASFESSIERDVPAWFNQDGLNHDRKNLGTLG
                     GGNHFIEVQASEDGTVWLMLHSGSRNLGHRIASFYHRLAQQLNQRWHADLPSPDLAFL
                     PLETGEGQGYVRDMTFALAYALENRRRMMAAAKSVMAEHLPGIGFVEEINIHHNYAKL
                     EHHFGRNLWVHRKGATSAREGELGIIPGSMGTPSYIVRGRGNPESFMSCSHGAGRSMG
                     RTEASRRLTLEECDRAMQGVVYDRWHKAGGGSKKRREKGIDYDFGEAPQAYKDIDQVI
                     AAELDLIEPLVKLRPLGVIKG"
     gene            1623..2120
                     /locus_tag="PKO06_03365"
     CDS             1623..2120
                     /locus_tag="PKO06_03365"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68710.1"
                     /translation="MWEQSCERNCGSSFWLFALRENIDFINEYLSHVGPVIRDNRGFI
                     DKYIGDAVMALFEQSPDDAVNAAIASLRPVVEFNELRQRRGEKPIRIGIGLNTGTLML
                     GTVGEHNRINATVISDAVNLASRVEGQTKTYGAEILITETTFKSLIRPHQYLIRTVDT
                     IQVKG"
     gene            2491..3696
                     /locus_tag="PKO06_03370"
     CDS             2491..3696
                     /locus_tag="PKO06_03370"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNV68711.1"
                     /translation="MEKKRIVIRSPQIYSGFCHIGSCMAFVWIVGEAGDHQFIFQDLA
                     CSDSLETLSLKKDLYLCAGTGEEYIIQDFRLDEVTNMKEIRGWASDIAHLNRSFVEAK
                     TELPDQKGMLSPKTQQKAIRTMSSMVNAVRGDARIQMREAQEAATQLLGEVSHTPHAI
                     VNLMAVRSFDDYTYAHSINVATLALTIGHGMRMSPSEMKILGMAALLHDVGLLKLPLE
                     LLHKEAQLTEEEFEQVRQHPQLGYDLVAPAVDLDPRIKQTVLHHHEKFQGGGYPKGLK
                     GHAIPLFARIVAIADVYDALTSPRPFRPQIYPYDAIRILLSNTENQFDPEILAVFVRR
                     MSIFPPGSMVRLNDGSEALVIRPNPGAHLKPVVRLVRDMQGMRAYTTNNLDLVQSDLY
                     VVGPGTSSK"
     gene            complement(3747..5333)
                     /locus_tag="PKO06_03375"
     CDS             complement(3747..5333)
                     /locus_tag="PKO06_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV68712.1"
                     /translation="MRSVTGSLPGRLLQAFALLVLLLLSSTAATARTGGEGRVSILFT
                     NDLHSELEPFAEASATALLGGIVRQAEMIAEIRQQTPNTLVVSAGDQVQGTPFFQVFR
                     GRAEAEALSLAGYQLVTLGNHELDLGLPALAETLAIASYSLICANVFSAADLRPVFRP
                     YRLVQVASLSIAVLGIIGDDAWNDYGALIRRRLVQLEPRAVLRPLVDHVRPMVDLVVV
                     LSHSGLDSDKLLARDVPGIDVIIGAHDHTVMATVTEIVSERAEPCSPPTLIVQAGDRG
                     RFLGRLDFIVTADRRRLHSYELLPVLTPTGDPTRSPHPVSALMASYSRRLREIMETPV
                     TQAPQGFFYDRDRRNHEFLPLGTLTCEAFRAITGAEIGFINSGGIRRGIPAGHVTLRH
                     LFEALPYDNTVTVVTLTGADLASLLEQQCQRRVSDQGGFQTAGVTATFDMSSNTLSNL
                     LVSGVMVEKRKRYRIATSSYLTTDPLRKGTFGPWLATLEDSGIVMRDALGKYLHEVRV
                     LPSLDTPPIKFLGPVSTGVR"
     gene            complement(5427..>5895)
                     /locus_tag="PKO06_03380"
     CDS             complement(5427..>5895)
                     /locus_tag="PKO06_03380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNV68713.1"
                     /translation="LVDGDLRKPVLHTFFFQSHQRGLTDYVLGEPLERVMLPTAQPNL
                     WLLPTGPLPPDPSDFLGSAAMETRFKELVDRFDLVLFDAPPIGLVSDVMNFAQRIDAI
                     LYLAPLGREKKALTLKGVEMLKLVAAPVIGLACNFVNYYEPITYKYGYTKYTT"
BASE COUNT         1367 a         1696 c         1726 g         1106 t
ORIGIN      
        1 gcatgggaag tcgtggtgac acagaattcg tgacggtgtg gtgccgaacg gcacgaaagg
       61 agcgatgcta tggaatggat aaaggaccga aaggaactgt ccgagtcggc tcgtgtgccg
      121 gtgaagtcct ggtgcgcaga acccgaacca gaagcattga agcaggcggc gaatctggcg
      181 gcacacccgg tcgtcgaaca tcatgtcgct ctgatgcccg actgccatgt cggctacggc
      241 atgccgatcg ggggcgttat cggctgtcgc gaagcggtca ttccgaacgc cgtcggcgtc
      301 gatatcggct gtggcatgtg cgccgtgaag acgtcagcaa cggtcgagca gatgcgtgat
      361 ctggctttca ttcgcgcgat tctcgaggac gtgaagaagc tggtaccggt tggcgagggc
      421 aacagccaca agcgacaaca ggagtgggac ggtttcgcgt cattcgaatc gagcatcgaa
      481 agagacgttc cggcctggtt caaccaggac ggactcaacc acgatcgcaa gaacctcggc
      541 acgcttggcg gcggcaacca cttcatcgaa gtgcaggcga gcgaagacgg aactgtctgg
      601 ctgatgctgc attcgggctc gcgcaacctc ggacaccgga tcgcgagctt ctatcatcgt
      661 ctggcgcaac agctcaacca gcgctggcat gctgatctgc cctctcccga tctggcgttc
      721 ctgccgctcg agacgggcga gggccaggga tacgtgcgcg acatgacctt cgcgctggcg
      781 tatgcgctcg agaaccgccg ccggatgatg gccgcggcga agtcggtcat ggccgagcat
      841 ctgcccggca ttggcttcgt cgaagagatc aacatccatc acaactacgc gaagctcgaa
      901 catcacttcg gccgcaacct ctgggtgcat cgcaagggcg cgacctcggc gcgcgaaggc
      961 gaactgggca tcatcccggg aagcatgggc acgccctcct atatcgtgcg cggtcggggc
     1021 aatcccgaat cgttcatgtc gtgctcgcac ggcgccgggc gctcgatggg ccgcaccgaa
     1081 gccagccgcc gcctgacgct cgaggagtgt gaccgggcca tgcagggcgt ggtctacgat
     1141 cgctggcaca aggccggcgg cggctcgaaa aagcgccgcg agaagggtat cgactacgac
     1201 ttcggagaag ctccgcaggc ttacaaggat attgaccagg tcatcgccgc cgaactcgac
     1261 ctgatcgaac ccctggtgaa actgcgcccc ctgggcgtta tcaagggctg accacccgcc
     1321 ccacagacac ccattccctc tcgccgcgca gggtcatttg gttctgtcca gttttcctct
     1381 tggcgtgagc gaatcggtga gcggtgccga ggggaaaacc caagccggag aatcaaatga
     1441 ccctgctcac cgcgtccttt tcattgactc gttcaaaacc ttggcggtat gttctgaatg
     1501 ggcttgggga cttcgtttcc cgtgatacag cgtctcggtt ttccgtcttt cggcttctgg
     1561 aacacctgtc ggcttgtcgt gaaaaccagc tcgggaatct gcgactctga ttcgagaatg
     1621 ctatgtggga gcagtcatgc gagagaaact gcggatcgtc gttctggctc tttgcgctca
     1681 gagagaacat cgacttcatc aacgagtatc tgagccacgt cggcccagtc atcagagaca
     1741 atcgcgggtt catcgacaag tatatcggcg acgcggtcat ggctctgttc gaacaatcac
     1801 cagacgatgc ggtcaatgcg gcaatcgcct cgctgcgacc ggtcgtcgag ttcaacgaac
     1861 tccgtcagcg tcgtggcgag aaaccgattc gtatcggcat cgggttgaac accggcactc
     1921 tgatgctggg aacagtcggc gagcacaacc gcatcaacgc gacggtcatc tctgatgcgg
     1981 tgaaccttgc ctcgcgggtt gaaggccaaa ccaaaaccta cggggcagag attctgatca
     2041 cggaaacgac cttcaagagc ctgatacggc cgcaccagta tctgattcgt accgtcgaca
     2101 cgattcaggt caagggatag aaccaggcgg tctgcattta tgaagtctgc gacgggtcag
     2161 agtcgaagaa acgtgagtat gtcgcggcga ttctttcgac ctaccaggag gcgatggccc
     2221 tggactacga aaagaaattt gccaaggccg aaaagcgctt cagagactgt ctcagggaag
     2281 gaacaagtga cgtctgcgtt aatctgtacc tcgatcgatg ccgatcatat ctagagagtc
     2341 cgccaccgga agactggaac ggcgtcaccc gattgcgcag caagtagtca gccatcgccg
     2401 gctcgatctg agatacggaa aacagaacgc cgggttttgc gtagtatctg gtagaggggt
     2461 ggattccact gccaggagtt ttttcggact gtggaaaaga aacgaatcgt cattcgaagc
     2521 ccgcagatct attccggatt ctgccatatc ggcagctgca tggccttcgt ctggattgtg
     2581 ggcgaggcgg gcgatcacca gtttatcttc caggatctgg cttgttcaga ttcactcgaa
     2641 accctcagcc tgaagaaaga cttatacctg tgcgccggta cgggtgagga gtacatcatt
     2701 caggacttca gactcgatga agtcacgaac atgaaggaga ttcgcggctg ggcctcagac
     2761 atcgcacatc tcaaccgcag ctttgtcgag gcaaagacgg aactccctga tcagaaggga
     2821 atgctctcgc cgaaaaccca gcagaaagct attcgcacca tgtcttcgat ggtaaacgcg
     2881 gttcgcggcg atgcgcgcat tcagatgcgc gaagctcagg aagccgcgac tcagcttctg
     2941 ggagaggttt cgcacactcc gcatgccatc gtcaatctga tggccgtgcg atcattcgac
     3001 gattacacct acgcacactc tatcaacgtt gcaaccctgg cattgacgat cggtcacggc
     3061 atgcgcatgt ccccttccga aatgaagatt ctcggaatgg cggccctgct gcacgacgtt
     3121 gggctgttga aactcccgct ggagcttctg cataaagaag cccaactgac ggaagaagag
     3181 ttcgagcagg tccgccagca cccacagctc ggatacgacc tggtcgcccc cgctgtcgat
     3241 ctcgacccgc gcatcaaaca gacggtgctg caccaccacg aaaagtttca gggcggcggg
     3301 tatccaaaag ggctcaaggg gcacgccatt ccgctgtttg cccgcattgt cgccatcgcc
     3361 gacgtgtacg acgccctcac cagtccgcgg ccctttcgac cgcagatcta tccctatgac
     3421 gccattcgca ttctgctgag caacaccgaa aaccagttcg acccggaaat tctggcggta
     3481 ttcgtccggc gcatgtcgat cttcccccca ggaagcatgg tccgactcaa cgatggctca
     3541 gaagccctcg tcatcagacc aaacccgggt gcgcacctga aacccgtcgt tcgcctcgtg
     3601 cgagacatgc agggaatgcg cgcttacaca accaacaacc tggacttggt tcagagcgat
     3661 ctttacgtgg tcggcccggg tacaagttca aaatagcgtt cagcttgacc gggcgatact
     3721 ctcgcagaaa gaggacaaac gcccgactac cgaaccccag tggaaacggg gccaagaaat
     3781 ttgatcggtg gcgtatcaag agacggaaga acacgcactt cgtgcaggta ctttcccaaa
     3841 gcatctcgca tgacgattcc cgaatcttcg agtgtggcca gccaggggcc aaacgttccc
     3901 ttgcgcagcg gatcggtcgt cagatacgac gaggtggcaa ttcgataacg ctttcgcttc
     3961 tcgaccatga ccccactcac cagcaggttc gagagcgtat tggacgacat atcgaacgtc
     4021 gcggttactc cagctgtctg gaatccgccc tgatcactga ccctcctctg gcactgctgt
     4081 tcgagcagag aggcgagatc cgcgccggtc aaggtgacga ccgtgaccgt gttatcgtag
     4141 ggaagcgcct cgaagaggtg gcgaagtgtc acgtggccgg cagggattcc gcgccggatg
     4201 ccgcctgagt tgatgaaccc gatctcggca ccggtgatgg cacgaaaagc ctcgcacgtc
     4261 agtgtcccga gaggcagaaa ctcgtgattg cgccggtcgc ggtcgtagaa aaagccttga
     4321 ggggcttgag tgacaggcgt ttccatgatc tcacggagac gccggctgta cgaagccatc
     4381 agagcgctga ccgggtgagg tgaccgcgtc gggtcgcccg tcggcgtcag caccggtaac
     4441 agctcatacg aatgcaacct gcgccggtcg gcagtaacga tgaagtcaag ccgtccgagg
     4501 aaacgacccc gatcacccgc ttgaacgatc aaggtcggcg gtgagcacgg ctcagcccgc
     4561 tcactgacaa tttcggtcac ggttgccatc accgtatgat cgtgggctcc gatgatgaca
     4621 tcgatacccg ggacatcgcg cgccagcagc ttgtcggaat caaggccgga atgcgaaaga
     4681 acgacgacca gatcaaccat cggccgaaca tgatccacca ggggacgcag cactgctcgt
     4741 ggttcgagtt gcaccaagcg tcttcgaatc agcgcgccat aatcgttcca ggcatcgtca
     4801 ccaatgatgc ccagcacggc aatggaaagc gacgcaacct gtacaaggcg ataggggcga
     4861 aataccggtc gcagatcggc tgccgagaag acattggcac agatcagcga gtatgaggct
     4921 atggcaagag tttccgcgag agccggcaga ccgagatcga gttcatgatt tcccagcgtc
     4981 accagctggt atccggccag agacaacgct tctgcctccg cgcgcccgcg aaagacctga
     5041 aagaatggcg tcccctgcac ctgatcaccc gcgctcacca cgagcgtatt cggcgtctgc
     5101 tgacgaatct cagcgatcat ctcggcctgc cgaacgatgc cgccaagaag tgcagtcgca
     5161 gaagcctccg cgaatggctc cagttcagaa tgaagatcgt tggtgaacag aatcgacacc
     5221 cggccttccc cgccggtgcg agccgtcgca gcagtcgacg agagcagtaa gaggaccaga
     5281 agagcaaagg cttgaagcag gcgaccgggc aacgagcctg tgaccgaacg catacacaca
     5341 ccagatttca gaaatgtcat gccatcagct tacacgagtt cgtcgcggtg ttcacgagaa
     5401 acagttcagg ccagcgatga agaagatcag gtcgtgtatt tcgtgtatcc gtacttgtag
     5461 gtgatcggtt cgtagtagtt cacgaagttg caggcgagac caatgaccgg agccgcaacc
     5521 agcttgagca tctcgacgcc cttgagggtc aaggccttct tctcgcgccc caacggggca
     5581 agatagagaa tagcatcgat acgctgggca aagttcatga catcggaaac cagaccgatc
     5641 ggaggtgcgt caaaaagtac aaggtcaaat cgatcgacaa gttccttaaa tcgcgtttcc
     5701 atggcagcag aaccgagaaa gtctgaagga tcaggtggaa gaggaccggt cggcagcagc
     5761 cacagattcg gctgggcggt cggcaacatc acacgctcga gcggctcacc gagcacataa
     5821 tccgtgagtc cgcgctggtg tgactggaag aaaaaggtgt ggagaaccgg ttttcgcagg
     5881 tcaccatcga ccagg
//
LOCUS       DAORHD010000101         5874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13764_length_5874_cov_3.803746, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000101 DAORHD010000000
VERSION     DAORHD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13764_length_5874_cov_3.803746"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="PKO06_03385"
     CDS             <1..976
                     /locus_tag="PKO06_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV68714.1"
                     /translation="DWAKDPARIDVPYAVKVAAIGDIHGSFHELAASLECLGMAKVAD
                     AEKMTLTWTGGKDLLVFTGDFTDRGKYTREVYEAVMDLEQQAAKAGGRVIALFGNHEA
                     LLLNGQVEKWANTLKPPKKQHYQNTIDSFTKAGLDFHQAISPKGKVGAWIRRRPIVAV
                     VNGFLFVHGGLPSPARSLSDITADFQEGLDAEAFSKGIFMEEKGPLWHREWWNDQALV
                     EDNLQRMGVRGVVFGHTIGALGTEGEIACIGDRVINIDIGMCPTYGNSKGGGLLITIE
                     KNGQMQFTAKYPDRPEKDLMKVPAPVVPAVCPRPRPANEVLPRTGTGG"
     gene            1193..2842
                     /locus_tag="PKO06_03390"
     CDS             1193..2842
                     /locus_tag="PKO06_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV68715.1"
                     /translation="MKRLFLTLLLMVVVLVPAFAKDSGTRFSSLSGTVEVAADADAKA
                     DTWKFGKMDMVLEVDDHIKTGEESSAILSFADLSTFVMKEETHIILSTPPDKDSKVQL
                     VAGKIWANVKKMAKDGSLEIEMSQAVAGIKGTNITCQSSPTEDRIQVLRGLAEVLVKE
                     TGEKISVPEGFEIVVKAGGKTDKQEVSVEDVQEEWKKQLENFGSSIQLNEVPDLIKEI
                     MEEELAAFTELSNEFKALTSGEANDPDVVSEFKKDAERFIGVLLEDGMRLAAARAKIE
                     NELASPDLPSGDRDKFQGYIRQIAEAGTKMQDYQNQIGGWLKTNFLLGAATTGGTEVE
                     QMTAAVTETWSSIESLMAEVSANPSGLSQDWFFEALDVCNEVMADLDSQAEELSAYLD
                     EHPNDQAASNLLAQIGKYQDEIGNLLKDFNVVEIDGAVLTEMQDSDDLLSESIAELGE
                     QIDAYNDAVSGQGDEVKLERSLGILNNFSQARRLYLSSQRLYDSIKRETSGQKFRTAE
                     MEELDQTYERITETYNQLGIAATELESRLSELQSELGKQLQ"
     gene            2865..4937
                     /locus_tag="PKO06_03395"
     CDS             2865..4937
                     /locus_tag="PKO06_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68716.1"
                     /translation="MINLKKLTKTLAILISLLTVAASVWALSMASSKALDAFRRYEPD
                     FRRSLQSLQDFARKAHQQKLEATDKKWFAIKEEADETMEFVQKRFDLLDDLYKSTLND
                     NPGDRAALMDGFSRIEDLYRRCRDLHVEKFQEGGALLNGAADSTEPAPVDLTKTEYEK
                     PVATGETAAKTEVPATSEPAKPETTTTEGTELKPVPPTPVTVAPESSSTAKPEPKEEQ
                     KVRLHGTFKADLRNRNEKYTATNRVLPSNFNQFKLSLSYQADEKNKLSLDNKYIHRKR
                     NELVKENILTFAWLHVHSKEMAWSLKDTLHHVWYPQATVKEYRDNLIEGFLNLKEKRW
                     ERTYNLGFDRRVYPNYSRSDYKQWNLGGQTTRFIPNGTVFWDSIYNGRNYDNSPSLDY
                     TNQYYNLEYNQSFNGNDSEVSVSNTYDTRHYGNEAVNLFRANYWDNYFRFRYELPVNK
                     KWTWIFEDDYQKRLYASDFSRGYSQFNAKTTAKITIDKQTRARFWHNYTMNHENTTAR
                     AHRNHKFNGMWEKKFSDSFKLKLEDTFHHRTAITGATLDFHENVFNAKATWKLPSKIE
                     LVWNNEYLTRYYEALFYRDYRYFATGLAATYAKAKQFEWQLGYQHRTFSFRNGNNLAT
                     GWNSNAQPLAEAKYTKYINKDLKAYVASSYEKTFYKTFDTLSQELLWDFTRPLTITEF
                     TGGLEYSF"
     gene            4951..>5874
                     /locus_tag="PKO06_03400"
     CDS             4951..>5874
                     /locus_tag="PKO06_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68717.1"
                     /translation="MKSTRVLVVFFGILLSIGLLGCGGGGGGGGGGTVAPDAPAITAR
                     LNSFVKAMAHGNAVEATKFMSSRLAAASGGGTLIIKDFGDDIDDPSDDKSYNFLIPSD
                     GILQSGDIATVRAEYTTDQSVTLSITFTLLREEGEWYIDDVEVTSSSTTTYRLADWFQ
                     VNPGNTWTYKIKYGGGLDSTYKVRYTVEPSTLSQTYTDTPTSTSNTVEAYSMKAEALD
                     ASGNPITPISSVAGTARGDDPEPRLSWNSSFEPETFSGDNGTLYFGVTGREVSADPWQ
                     MIPEEVTLGTSYTPSTSFNAPNGSPHTPRTDI"
BASE COUNT         1432 a         1597 c         1661 g         1184 t
ORIGIN      
        1 tgactgggcg aaagacccgg ctcgcattga tgtgccgtat gccgtgaagg ttgccgcgat
       61 cggggacatt catggttcgt ttcatgagct tgcggcgtcg cttgaatgtc tgggcatggc
      121 caaagttgcc gatgccgaga agatgacgct gacctggaca ggcggaaaag atctgctcgt
      181 gttcaccggt gacttcactg atcgcggcaa atacactcgc gaagtgtacg aagcggtcat
      241 ggatctcgaa cagcaggcgg cgaaggccgg tggacgcgtg attgcgctgt tcggcaacca
      301 cgaagccctg ctgttgaatg gtcaggtcga gaagtgggcg aacaccttga agccgcccaa
      361 gaaacagcac taccagaata cgattgattc gttcacgaag gccggtcttg atttccatca
      421 ggcgatttca cccaaaggca aggtgggtgc ctggattcgt cgccgcccga tcgttgccgt
      481 ggtcaatggt tttctgttcg tgcatggcgg actgcccagt ccagcccgct ccctttctga
      541 catcacggcc gactttcagg aaggcctcga cgccgaagcc ttctcgaaag gtatttttat
      601 ggaggaaaaa ggcccgctct ggcaccgtga atggtggaac gatcaggcgt tggtcgaaga
      661 caacctgcag cgcatgggtg tgcgcggcgt ggtgttcggt cacacgattg gcgcactcgg
      721 caccgaaggc gaaatcgcct gcattggtga ccgggtcatc aacatcgaca ttggcatgtg
      781 ccccacgtat ggcaacagca agggcggcgg attgctgatc accattgaaa agaacggtca
      841 gatgcagttt accgccaagt atcctgatcg cccggaaaaa gacctgatga aagttcccgc
      901 gcccgtcgtg ccggccgtgt gcccgcgacc ccgcccggcg aacgaggtgt tgccgcgaac
      961 tggcaccggc ggctgatatt cagctggcct gccgggtgcg cacacagcgc ctggcgcgac
     1021 agacaagaac aagacccggg gtactcgccc cgggtttttt gctgcttgac aatccttcag
     1081 ccacgactct gaaaatagtg tacgggaaaa ttgactcaac tattccagtg tgctaacctc
     1141 ggtcgttctt gtccctggac ggggccctac atgcagttcg aaggagtgag agatgaaacg
     1201 gcttttcctg accctcctgc tcatggtggt ggtgctggtt cccgcttttg cgaaagattc
     1261 gggaactcgg ttttccagcc tttccggcac ggttgaagtg gctgctgatg cagacgccaa
     1321 ggctgatacc tggaagttcg gcaagatgga tatggtgctt gaagtggatg accacatcaa
     1381 gaccggcgaa gagtcgagcg cgattctgag ctttgccgat ctttccacct ttgtcatgaa
     1441 ggaagagacg cacatcattc tctcgacccc gcctgacaaa gacagcaaag tgcagcttgt
     1501 agccggaaag atctgggcca acgtcaagaa gatggccaaa gacggctcac tcgagatcga
     1561 gatgagtcag gccgttgccg gtatcaaggg cacgaacatc acctgtcaga gcagcccaac
     1621 ggaagatcgt attcaggttc tgcgcggtct cgccgaagtg ttggtcaaag aaaccggtga
     1681 gaagatcagc gttcccgagg gttttgaaat tgtcgtcaag gcaggcggca agactgacaa
     1741 acaggaagtg agcgtcgagg acgtccagga agaatggaag aagcaactcg agaacttcgg
     1801 tagttcgatt cagctcaatg aagtcccgga tctcatcaaa gagatcatgg aagaagagct
     1861 cgctgccttt accgagctct cgaacgagtt caaggctctt acttcgggtg aggccaatga
     1921 cccggatgtc gtgtccgagt tcaagaagga cgccgaacgg ttcatcgggg ttctgctcga
     1981 ggatggcatg cgtctggcgg cagcccgagc gaaaatcgaa aatgagctgg cgtcaccaga
     2041 tctaccgagc ggcgatcgtg acaagttcca gggctacatt aggcagattg ccgaagccgg
     2101 aacgaaaatg caggactacc agaaccagat tggcggatgg ttgaagacca acttcctgct
     2161 gggtgcggcc acgactggcg gcaccgaggt cgaacagatg accgctgccg tgactgagac
     2221 ctggtcatcg atcgagtcgc tcatggccga agtcagcgcg aatccgagcg gcttgtcgca
     2281 agactggttc tttgaagcgc tcgatgtctg taacgaggtc atggctgatc tcgattccca
     2341 ggcagaggag ttgagcgcct atctcgatga acatccgaac gatcaggcgg cttcgaatct
     2401 tctggcccag atcggcaagt atcaggacga aatcggaaac ctgctgaagg atttcaatgt
     2461 cgtcgagatc gacggagcgg tgttgaccga gatgcaggat tcggatgatc tgttgtctga
     2521 atccattgct gaactcggcg agcagatcga tgcctataat gatgccgtat cgggtcaggg
     2581 cgatgaggtg aagctggagc gttcgttggg tattctcaac aacttctcgc aggctcgccg
     2641 tctgtatctc agcagtcagc gcctgtacga ttccatcaag cgcgaaacca gcgggcagaa
     2701 gttccgcacc gccgaaatgg aagaactgga ccagacctac gagcgcatta ccgaaaccta
     2761 caatcagctc ggcattgccg cgaccgagct cgagagccgg ctctctgaac tgcagtccga
     2821 gctgggtaag caattgcagt aagcgttgaa aggaaaacac ggatatgatc aacctgaaaa
     2881 aactgaccaa gactctggcg attctgatca gtctgcttac tgtggcagct tcggtgtggg
     2941 ctctgtcgat ggcgagttca aaagcgctcg atgccttccg gcgctacgaa cccgactttc
     3001 gtcgcagcct gcagtccctg caggatttcg cgcgcaaagc gcatcagcag aaacttgaag
     3061 ccaccgacaa aaagtggttc gcgatcaagg aagaagcgga cgaaacaatg gagttcgtgc
     3121 agaagcgttt cgatctgctc gacgatttgt acaagtccac tctcaacgac aaccccggcg
     3181 atcgtgctgc gcttatggac gggttttcac gcattgagga cctgtaccgc cgctgtcgcg
     3241 atcttcatgt tgaaaaattc caggagggtg gcgccctctt gaacggtgct gctgattcga
     3301 cggaaccggc cccggttgac ctgaccaaaa ccgagtatga aaaaccggtt gcgacaggcg
     3361 agaccgccgc gaagaccgag gttcccgcca cgagcgaacc ggccaagccc gaaacgacca
     3421 caactgaagg cactgagctg aagccggttc cgccaactcc ggtgacggtt gctcccgaga
     3481 gcagctcgac cgcgaaaccc gaacccaaag aagaacagaa agttcggctg catggcacgt
     3541 tcaaggccga tctgcgcaac cgcaacgaga aatacaccgc gaccaatcgc gtgttgccga
     3601 gcaacttcaa ccagttcaag ctgtcgttgt cgtatcaggc cgatgaaaag aacaaactct
     3661 ccctcgacaa caagtacatt caccgcaagc gcaacgagct ggtgaaagaa aacattctga
     3721 ccttcgcctg gctgcatgtg catagcaagg aaatggcctg gtcgctcaag gatacgctgc
     3781 accacgtctg gtatccgcag gcaacggtga aggaataccg cgacaacctg atcgaagggt
     3841 tcctgaacct gaaagagaag cgctgggaac gaacatacaa cctcgggttc gaccgccgcg
     3901 tctacccgaa ctactcgcgg tccgattata aacagtggaa tctgggtggc cagacgaccc
     3961 gcttcattcc gaatggcacc gtgttctggg attccatcta caatggccgc aactacgata
     4021 actcgccgtc tctcgactac acgaaccagt actacaacct ggaatacaat cagtcgttca
     4081 acgggaacga ctccgaagtt tcggtgtcga atacctacga tacgcgtcac tatggcaatg
     4141 aagcagtcaa cctcttccgc gccaactact gggacaacta cttccgtttc cggtatgagt
     4201 tgccggtgaa caagaagtgg acctggatct tcgaggatga ctatcagaag cgtctctatg
     4261 ccagcgactt cagccgcggg tattcgcagt tcaacgccaa gacgaccgcg aagatcacca
     4321 tcgacaagca gacccgtgct cgtttctggc acaactacac gatgaatcac gaaaacacga
     4381 ccgcccgcgc gcatcggaac cacaagttca acggcatgtg ggaaaagaag ttctcggatt
     4441 cgttcaagct gaaactggaa gataccttcc atcaccgcac ggcgatcacg ggtgctactc
     4501 ttgatttcca cgaaaacgtc ttcaacgcga aggcgacgtg gaaactgccg agcaagatcg
     4561 aactggtctg gaacaatgaa tacctgaccc gatattacga ggccctgttc tatcgagatt
     4621 accgctactt cgcgaccggt ctcgccgcga cctatgccaa agcgaaacag tttgagtggc
     4681 agctcgggta ccagcaccgc accttttctt tccgcaatgg gaacaatctg gcgacgggtt
     4741 ggaactcgaa cgcgcagccg ctggctgaag ccaaatacac caaatacatc aacaaggatc
     4801 tgaaggcgta tgtggccagc agctacgaaa agaccttcta caaaaccttt gatacgctct
     4861 cacaggaact gctgtgggat ttcacccgac cgctgacaat aacggaattt accggcggcc
     4921 tggaatattc attctgagag gatgaagggt atgaagtcga ctcgtgtcct ggtcgtattt
     4981 ttcgggattc tcttatcgat cggtcttctc ggctgcggtg gcggcggagg cggtggcggg
     5041 ggtggtacag ttgcacctga cgcgccggcg atcacggcgc gtttgaactc gttcgtgaag
     5101 gccatggctc atgggaacgc ggtcgaagcg accaagttca tgtcctcgcg gctggccgcg
     5161 gcaagcggcg gtggcacgtt gatcatcaaa gacttcggcg atgacatcga tgatccgagt
     5221 gacgacaaat cgtacaactt cctgataccc tctgatggca tcctccagtc aggtgatatc
     5281 gcgacggtga gggcggaata cacgactgat cagagtgtca cgctgtcgat aacgttcacg
     5341 ttgctgcgag aagagggaga gtggtacatc gacgatgtcg aggtcacctc ttccagcacg
     5401 accacgtatc gtctggctga ctggtttcag gtcaaccctg gaaacacctg gacctacaaa
     5461 attaaatacg gtggtggtct cgactcgacc tacaaggtgc gctatacggt tgaacccagc
     5521 actctttctc agacctacac ggacacgccg acctcgacat cgaacacggt cgaagcctac
     5581 tcgatgaaag ccgaagcgct cgacgcgagc ggcaatccga tcacgccgat ctcttctgtc
     5641 gctggaacag cgcgtggaga cgatcccgaa ccccgtttgt cgtggaactc gagttttgaa
     5701 ccagagacgt tctctggcga taacggaacg ctctatttcg gtgtgaccgg acgagaagtc
     5761 tcggctgatc cgtggcagat gattcccgaa gaagtgacgc tgggaacatc ctatacgccg
     5821 tctacttcct tcaatgcccc gaatggctct ccccatacgc cgagaacgga catt
//
LOCUS       DAORHD010000102         5843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13866_length_5843_cov_3.239288, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000102 DAORHD010000000
VERSION     DAORHD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13866_length_5843_cov_3.239288"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKO06_03405"
     CDS             complement(<1..808)
                     /locus_tag="PKO06_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68718.1"
                     /translation="MTQPTADLHLNPQGAVSAPAKPAGPPAQPSSFILAGAIMLLFTG
                     LIASGILAFQMNDLQVFYNLSFAVFCCYCGFIGWYNGIIRELAVLFHYVAPFVIGYTQ
                     ARPLALHFGHTSWFAILGAFWACVFGSFVAIFILLYLLRALLWPTSFPGRVAGLALGL
                     SEAMLLFIAIGNIYQAVPFADKELGQYLKPVMDSIAEKLSTPTLQFAAATTRDITDIT
                     LQIRKNGFDPNRVDQPALRRNFEPLVNSPSVQRLVDDQALMEKLRAGKSLE"
     gene            complement(805..3780)
                     /locus_tag="PKO06_03410"
     CDS             complement(805..3780)
                     /locus_tag="PKO06_03410"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNV68719.1"
                     /translation="MITRKSGQRGFALSVVLLALVTVFVTVHPPVLTAAELILESDTP
                     FLDPSFSPDGTKIVCIGEDQGQPQVVLFDRVAGKVRILTSSKGEKRTPCFAGDEKTIF
                     FCGKEKGNFDIFKVGIEGGKEVNLTRTPENEFACEVSVNVFEDEDEKRFYRVYYLKGK
                     DKSGALWSITDNGKNPREEIAGAFQAFTLHSDLRSIVLSDGKALLSALYLVSSTGEGI
                     ITVDAPAKPVAVPGSAGLKEPVFTGNDLHLAVRRAKSAVLLDPSTATTQSLTTQPWIS
                     RPAFSRARPEMALIIKNGNRYRLDLVPLTQPELRIRNLYKFPGNRFFTLNDSDAAWNK
                     IKAQSFVVIPDKIREFFMLYESNRYEWLDSYITIDAAFHLFHLFYDYTLKEIERGTFR
                     AGIASCSAALAHEAALAVEAATNPQAKSCLQTLQGVFTLAALLYEPDPAALQTAATKV
                     PAAIRESLTHDLEKILNANENCRSKLLGRPVDFTQFKVRGHYEGEPALESYFRTVMLF
                     GQFGLRVASPDGTAPSEDLPLILALAWLGQKAKVQGKPVSEVLADLSAPIDFLVGEPE
                     DLPFPTLAKTMGEQQLPTNADDCVDPQRLKAVFETLKQYPRPRIRPQDGVQVFVFPQR
                     ATLDSIIMQQLVYRAVGTQAKPRWLPRLLDVMAGLGSARAKALLLDTLHEGQYDRYES
                     QLEKATREVAGLKESTWTDNLYRGWLGAFRDLCLNTDQPTMAFAQSPAWTDRILYAAL
                     GSLTTLRHDTLLYNKMGGAEAGEGGERMYRKFPRVFVDPYPSVYARMRRLTLELYGLM
                     KARGFLPPEDKKDDTEETDMILGASLNGLMKSLAGILEKLEAAARKQARGEVLSEAEQ
                     AALFEIGRDLEHLTIALTAKPEDGFGNIYNDECSLIADVFTGDETVRECAVGRVFNLF
                     VRHAANTGFEYLYRGGTYSFYEFEQPLSNRLSDKQWKKMIRDNQLPPLPEWSRSFIVG
                     DPPKIDPDEEYYPGMEENQ"
     gene            complement(3777..4307)
                     /locus_tag="PKO06_03415"
     CDS             complement(3777..4307)
                     /locus_tag="PKO06_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68720.1"
                     /translation="MSLIWLMQFWLFWLTACPVGDAITLARECYILPTPAGRQFFYLS
                     SRPVHQPTETIIARHTSPFSPPLCRYRTRPVFKLRSGDIDGDGREELIAGVTRPDLGH
                     QRRIYVFTLDDRLMRPRWFGTRLSFHLDWFDTIPVGSRVGLTTREHGRKFRYRIVYRW
                     YYWGFRTVRIQKEDQP"
     gene            4455..5687
                     /locus_tag="PKO06_03420"
     CDS             4455..5687
                     /locus_tag="PKO06_03420"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNV68721.1"
                     /translation="MILVLNCGSSSLKYEVFKMPHRQSLGRGLIERIGEVKGRLTQTS
                     ERGKIEIGDVIPNHQAAMELMAKALVDSQSGILKSIKEIVGIGNRVVHGGEKYAASVV
                     IDDDVIATVEKCAELAPLHNPPNLMGIRAAYELLPEVKQVAVFDTAFHQTMPPAAYMY
                     GLPYEVYEKYRVRRYGFHGTSHRFVAARACAIMKRAPENTNLITCHLGNGASVTAIAN
                     GKSIDTSMGFTPLEGVLMGTRCGDIDPAILPYLEERGFSPKELNSMMNKKSGLLGLSG
                     ISNDLRDLEVAAEKGNKRAQMALDVYAYKIRRYVGAYSANLVKNDILVFTGGIGQNGV
                     KMRERICHRLENIGIMMDYSLNRVLGSKEGVVSKSYSPVAILVVPTNEELQIAIDTFD
                     LLFHEKAEFRRRASDVNL"
BASE COUNT         1265 a         1573 c         1811 g         1194 t
ORIGIN      
        1 cttcgagact cttgccggcc cgcagttttt ccatgagcgc ctgatcatcg accagacgtt
       61 gtacggaagg actgttgacg agaggttcga agttgcggcg cagggcgggc tgatcgaccc
      121 gattcggatc gaacccgttt ttacggatct gcagcgtgat gtcggtgata tcccgggtgg
      181 tggccgccgc gaactgcaga gtcggggtcg acagtttctc cgcgatcgaa tccatgacgg
      241 gcttcagata ttgtccgagt tctttgtcgg caaatggcac tgcctgatag atgttcccga
      301 tagcgataaa gagaagcatg gcttcagaca acccgagagc gagccccgcg acccggccgg
      361 gaaagctggt cggccagagc agcgcccgca ggagatacag cagaatgaag atcgccacga
      421 aggagccgaa gacacacgcc cagaaagctc ccagaatggc aaaccacgag gtatgaccga
      481 agtgaagggc gagggggcgg gcttgagtat agccgatgac gaacggagcg acgtagtgaa
      541 acaacaccgc cagttcccgg atgatgccat tgtaccagcc aatgaaaccg caatagcagc
      601 agaaaacggc gaaagagaga ttgtagaata cttgcagatc attcatctga aacgccagaa
      661 tgccgctggc gatcaggccg gtgaagagca gcatgatcgc accggcgagg atgaaactgc
      721 tgggttgggc cggtggaccg gccggcttgg ccggggcgga taccgccccc tgagggttca
      781 gatgcagatc ggcggttggc tgggtcattg gttttcctcc atgccggggt agtattcctc
      841 atcaggatcg atcttcggcg ggtcaccgac aataaaggaa cgcgaccact cgggcagcgg
      901 gggcagctgg ttgtcgcgaa tcatcttctt ccactgcttg tcggaaagac ggttgctgag
      961 aggctgttcg aactcgtaga acgaataggt gccgccgcga tagagatact cgaacccggt
     1021 gtttgcagca tggcggacaa acaggttgaa cactctgccg acggcgcact cgcgcacggt
     1081 ctcgtcgccc gtgaagacat ctgcgatcag tgagcattcg tcgttgtaga tgttgccgaa
     1141 cccgtcctct ggtttggcgg tcagcgcgat ggtcagatgt tcgagatcgc ggccgatttc
     1201 gaacagcgcc gcctgctccg cttccgagag aacttcacca cgggcctgtt tgcgggccgc
     1261 cgcttccagt ttctcgagta tgccggcaag agacttcatc agaccgttca gcgacgcgcc
     1321 gagaatcatg tctgtttctt ccgtatcgtc cttcttgtct tccgggggca ggaagccgcg
     1381 ggctttcatc aagccataga gttcgagcgt caggcggcgc atgcgggcat acaccgacgg
     1441 gtacggatcg acgaagacgc gggggaactt gcgatacatg cgctcaccgc cttctccggc
     1501 ctctgcgcca cccatcttgt tgtacaggag cgtgtcgtgc cgcagcgttg tcaggctccc
     1561 gagagcggcg tagagaatgc gatcggtcca ggccgggctc tgggcaaagg ccatggtcgg
     1621 ctgatccgta ttcaggcaaa gatcgcgaaa agcgccgagc cagccgcgat agaggttgtc
     1681 ggtccaggtc gattccttca gcccggcgac ctctcgtgtg gctttctcga gctgcgactc
     1741 atacctgtca tactggcctt cgtgcagagt gtcgagcagc aatgccttgg cacgtgccga
     1801 acccaggccc gccatgacat cgagcagtcg cggcagccag cgaggcttgg cctgagtgcc
     1861 aacggcacga tagacaagct gctgcatgat gatggagtcg agggtggcac gctgcggaaa
     1921 aacgaagacc tgcacgccgt cctgcggacg aatacgtggg cgggggtact gtttcagggt
     1981 ctcgaagact gccttgagac gttgcgggtc gacacagtcg tctgcgtttg tcgggagctg
     2041 ctgttcaccc atggttttcg ccagggttgg aaacggcagg tcttcaggtt caccgaccag
     2101 aaagtcgatc ggggcggaga ggtcggcgag cacttcagac acaggtttgc cctgtacttt
     2161 tgctttttgt ccgagccagg cgagcgcgag aatcagtggg agatcttcgc tgggcgcggt
     2221 gccgtcgggg ctggccactc gaagaccaaa ctgaccgaag agcatgaccg tgcggaaata
     2281 gctttcgaga gccggttctc cttcatagtg accgcgaacc ttgaactggg tgaaatcgac
     2341 cgggcgcccg agcagcttcg accgacagtt ctcgttcgcg ttcagaattt tctcgagatc
     2401 gtgagtcaga gactcgcgga tcgccgctgg cactttggtc gccgccgtct gcagtgctgc
     2461 ggggtcgggc tcatacagca gggcggcgag cgtgaagaca ccctgcaggg tctgcaggca
     2521 ggacttggcc tgcggattgg tcgccgcctc aaccgccagc gctgcctcgt gggcgagcgc
     2581 ggcgctgcac gaggcgattc ccgcccggaa agtgccgcgt tcgatttctt tcagggtgta
     2641 gtcgtagaac agatgaaaca gatgaaaggc ggcatcgatg gtgatgtaag aatcgagcca
     2701 ctcgtaccga ttgctttcat acagcatgaa aaactcgcga atcttgtcgg gaatgacgac
     2761 gaagctttgg gccttgatct tgttccaggc ggcatcagag tcgttcaggg tgaaaaaacg
     2821 attgcctgga aacttgtaca aattacgaat gcgcaactcg ggttgtgtca gaggaaccag
     2881 atcgagtcgg tagcgatttc cgttcttgat gatcagcgcc atctcaggtc gagcccgtga
     2941 aaaggcgggt cgcgagatcc agggctgtgt ggtcagagac tgggtcgttg ccgtgctggg
     3001 gtcaagcagc accgcacttt tggcgcgacg taccgcgaga tgcagatcgt ttcccgtaaa
     3061 caccggttct ttcagacccg cactgccggg aacggcaacc ggtttagcgg gcgcatcgac
     3121 ggtgatgatc ccttcgccag tagaggaaac gagatagagt gctgacaaca gcgctttgcc
     3181 gtctgacagg acgatggacc gaagatccga atgcagagtg aaggcctgaa acgcaccggc
     3241 gatctcttcg cgcgggtttt taccgttatc cgtgatcgac cagagagctc cagacttgtc
     3301 cttgcccttc aggtaataga cacgatagaa acgcttttca tcctcgtcct cgaatacgtt
     3361 gactgagacc tcgcaggcga attcattttc gggagtccgg gtcagattta cttctttccc
     3421 gccttcaatg ccgaccttaa agatgtcgaa attgcctttc tctttgccgc agaagaagat
     3481 ggttttctcg tcgccggcga aacacggggt gcgcttctct cccttcgagg aggtgagaat
     3541 gcgcaccttg cccgccacac gatcaaacag aaccacttgg ggttgtccct gatcctcacc
     3601 gatacacacg attttggtgc cgtcggggga aaaagacgga tcgaggaagg gggtgtccga
     3661 ctcgagtatc agttcggctg ccgtcagaac tggtggatgg actgtaacaa agacggtgac
     3721 gagtgccaac agcactacgc tcaatgcgaa accccgttga cctgatttcc gtgtgatcat
     3781 ggctgatcct ccttctgtat tcgaaccgtg cgaaaccccc aataatacca tcgatagacg
     3841 atacggtacc ggaattttcg accgtgctcg cgcgtcgtca gaccgactcg tgagccgacc
     3901 ggaatcgtgt cgaaccagtc gagatggaaa gacaggcggg tgccgaacca gcggggccgc
     3961 atgagacgat cgtcgagggt gaacacgtag atacggcgct ggtgcccgag atccgggcgg
     4021 gtgacgccag cgatcagttc ttccctgcca tcgccgtcga tgtcgcccga acgcagcttg
     4081 aagaccggtc gggtgcggta gcggcacaga ggcggtgaga acggagaggt gtgccgtgcg
     4141 atgatcgtct cggtcggctg atgaaccggc cgggacgaga ggtagaaaaa ctgtcgaccg
     4201 gccggagtcg gcagaatgta acactcgcga gccaaggtga tcgcgtcgcc cacggggcat
     4261 gccgtcagcc aaaacagcca gaactgcatc aaccagatca gcgacacgtc gaccactcct
     4321 cggacttatt cggggtcagc gacaacggat gaattctacc atgggttttg cttgacctcg
     4381 tggcgcgcac gttctataac ggagacaaac acgtggggag gtcgagcatg gacatgccgc
     4441 agaatgaggg tcgaatgatt ctggtgctga attgcgggag ctcttcgctg aagtatgaag
     4501 tgttcaagat gcctcatcgg cagagtttgg gccgtgggct catcgagaga atcggtgaag
     4561 tgaaggggcg actcacccag acctcagaac gcggcaagat cgagatcgga gatgtcattc
     4621 cgaaccatca ggctgccatg gagctgatgg ccaaagcact tgtcgattct cagtcgggta
     4681 ttctgaagtc gatcaaagag attgtcggca tcggaaaccg tgtggtacac ggaggcgaga
     4741 agtatgcggc atcggttgtc atcgacgacg atgtgattgc caccgtcgag aagtgcgcag
     4801 aactcgcgcc gttgcacaat ccaccgaacc tgatgggcat tcgcgcggcg tatgaactgc
     4861 ttccagaggt caaacaggtc gcggtcttcg atacggcttt ccaccagacg atgcctccgg
     4921 ccgcgtacat gtatgggctg ccgtatgagg tctacgagaa gtatcgcgtg cgacgctacg
     4981 ggtttcacgg aaccagtcat cgcttcgtgg cggcacgggc ctgcgcgatc atgaagcgcg
     5041 cccctgagaa caccaatctg atcacctgcc atctgggcaa cggagcctcg gtcacggcca
     5101 ttgccaatgg caaatcgatc gacacctcga tgggatttac accgctcgaa ggggtcctga
     5161 tgggcacgcg ttgcggagat atcgatccgg cgattcttcc ctatcttgag gaacgcggct
     5221 tttccccgaa agaactgaac tcgatgatga acaagaagag cggtttgctc gggctttccg
     5281 gcatctcgaa cgacttgcgt gatctcgagg tcgcggccga gaaggggaac aagcgggcgc
     5341 agatggcgtt ggatgtctac gcctacaaga ttcgtcgcta tgtcggggcc tattcggcga
     5401 acctggtgaa gaacgacatc cttgtcttca cgggcggcat cggccagaat ggcgtgaaga
     5461 tgcgtgaacg catctgccat cgactcgaaa acatcggcat catgatggac tattccctga
     5521 accgcgtgct gggttcgaaa gaaggggtgg tatccaagtc gtattcgccg gtcgcgattc
     5581 tggtagtgcc gaccaatgaa gagctgcaga tcgcgatcga tacgtttgat ctgctgtttc
     5641 atgagaaggc cgagttccgc cgccgagcgt cggatgtgaa tctctgaccg gtcagacccg
     5701 aaccccgcaa gccagacaat gaaatttgtg tggattgcgg gttttctctt tctgtcactc
     5761 ctcgatcgat ctgattctgc gaggtcgctc gggagaggac ccgcagtcgg ccacactggt
     5821 actcttcgaa gtacgttttc ggc
//
LOCUS       DAORHD010000103         5835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13905_length_5835_cov_3.782353, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000103 DAORHD010000000
VERSION     DAORHD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13905_length_5835_cov_3.782353"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKO06_03425"
     CDS             complement(<1..1221)
                     /locus_tag="PKO06_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68722.1"
                     /translation="MRHLLRPLVVFLAVVLVSFCVGCGGGGGGGDWGGGGINQIAVRN
                     AIGGGNIRGDIRAAAAAVPATAKVSLYQNGVLVSGPQDMGAAGVVFTGLVDGTYSVRI
                     TVGARIFQSSKTVSSTRTALIARVSYFNTSGLGILFEEILDQNSDGLAGSGENPVTRE
                     FSSGATSEATLYDAVDVTNWGAGLTGDSDGDGLINAFDEDIDGDGTLNSDESAADDDL
                     DDDNANNDTDTDDDQDGIPDDQDQQPGGTGTDVQLIDGPAGFKTAGFPAVSPTMTIPT
                     FQSPRKSAVVLVNTTSGQLTVTVAANGTGIVANQMVSANRSAQDEAMSDYERNLARLR
                     EIGNSLPAFRKTPVRLDIRANRAAVVNDQRTFYNGMERKSVTATLKNVSGSANGATGL
                     EHWVSNEIAGADLVT"
     gene            complement(1492..2064)
                     /locus_tag="PKO06_03430"
     CDS             complement(1492..2064)
                     /locus_tag="PKO06_03430"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNV68723.1"
                     /translation="MKRLTILFGSPRKTSNTRILAEEAARGAIEKGGEVEWLYLNDLR
                     INTCQSCNACKAEPITRWCVQQDDMSRVYELIDRSDALLIASPIYFGYVPALVKACLD
                     RLYPLVDPGFAPREANGKRVGFLYVQSHPDARQFLSPIRMFEAMFTLMGFEMYDRLIA
                     PDLDQGRKPMADKHPDLMEAALSLGRGFFA"
     gene            2162..2491
                     /locus_tag="PKO06_03435"
     CDS             2162..2491
                     /locus_tag="PKO06_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV68724.1"
                     /translation="MFWIWVAVIAVIIVISKCFSLFVFPRILSRWFTLHHELSTEDGY
                     VAVPDLSHLVGKQGVAVTDLKPCGKIRLDDTTYDARVEKGVISRGAKIVVSHAQGNSI
                     IVQPAEI"
     gene            complement(2605..5250)
                     /locus_tag="PKO06_03440"
     CDS             complement(2605..5250)
                     /locus_tag="PKO06_03440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV68725.1"
                     /translation="MSRVHIRFVISLLVCVLALASQASALIKPQPQRAILPNGLRVVV
                     QEDHSLPLVSCKLLFKVGPGVARPVAPGLVAVLARLLETAAPVGETRSAFLTELAKQG
                     CHPPQVVDTAQGLILMIQGPSDSLDVALRSLRRLAFGQTFSREAFDRAKNLELQKARQ
                     RLQFPLSSGYLSDAIAPMLFEGLPMGARFFATQEEVGKLTLADVEKMYQEQCVSNNAA
                     LVVVGDVMACEIFKGLMPVYGDLTALPVAEAKPAPAYRERADEKSIVQPDAVCTASVV
                     EKREYLDIKNTQVVIGFPAPALDDPDCAAASVWLIAMSHVNDSWLGRVNRHDFPKLSN
                     LSAQYQPYVGKGTFLISFESPDVDVDVVVSGLLRKLSALWRVPPRDAELQRILANCKS
                     IRSLKSEFRAVQGISLGIGELAGDFSLESSIWSAIENVSADDLARVARRMFTNGLCAV
                     RIAHPISAKSRQNAVVQSANLDNGMQVVVKPYGGSDVVAVCLRVGEPGGSFRKPMKGL
                     PELVFRMIEAQAGPEARDTQLARRLDEIGGLVHCQVIPGSGLYVVGYAGVDQYEALLQ
                     CLKSQVFAPVFDDKLLARLAAKVKSERETLLGQASVLMLEKFTALAFPGDEWKHSQVP
                     PENVAATKLTEARDFLKTWVKPGNVLCTVVGNLDPADGLRQARAVFGDLSSTEGPTRS
                     GGDGALLAGLEKTSEVEVRLPAGSAYLAGGYRLPPARASRHDPIPLDFITLGSLMHYL
                     ARSEDSLLQRELVQKGLAEAVEDVVFRHTHRASYLYFLVRVSPDKLEQCREELRRILR
                     QVPETQITAEQVEIAGKVVPNMFLQKMESSWYHALTLAGFLAQGYQADFFEKLPEAFK
                     QVKPEQISAAMREYARHYILITGRP"
     gene            complement(5277..5624)
                     /locus_tag="PKO06_03445"
     CDS             complement(5277..5624)
                     /locus_tag="PKO06_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68726.1"
                     /translation="MRKTVYLLSAFLLLASLFAASAKMVSYKLELRITTDRYEEYPFE
                     IGIDDREYRRITENVHTGIQPFLLEARKAIADRAGYRVEIYGADNYKMVVVRSYVVII
                     RDLSQGRVVFSKE"
BASE COUNT         1279 a         1781 c         1639 g         1136 t
ORIGIN      
        1 cgtgacaaga tcagcgccgg caatctcgtt ggaaacccag tgttcgagcc ctgtggcacc
       61 attcgctgag ccagagacat ttttcagtgt tgcagtgaca ctcttacgct ccatgccgtt
      121 ataaaatgtt cgttggtcat tcacgactgc cgcacgattg gcccgaatat ccaatcgaac
      181 cggtgttttg cgaaatgcag gtaaagagtt cccgatttcg cgcagacgcg ccaagttgcg
      241 ttcatagtct gacatagctt catcttgcgc ggatcgattg gcagaaacca tctggttagc
      301 cacaatccct gtaccattcg ccgccacagt cacagtgagc tgccctgatg ttgtattcac
      361 gagcacaact gcagactttc gtggagactg aaaggtgggt atggtcatgg ttggcgaaac
      421 ggcgggaaaa ccggcggtct tgaaaccagc cggcccatcg atcagctgta cgtcggttcc
      481 cgtgccgcca ggctgttgat cctggtcatc aggaatgccg tcctggtcat catcggtgtc
      541 ggtgtcgttg ttggcgttgt catcatcaag atcgtcgtca gcagctgatt catcagaatt
      601 cagcgttcca tcgccatcga tatcttcgtc gaaagcgttg atcaatccat caccgtcaga
      661 atcgcccgtg agtcctgcgc cccaatttgt cacatcaacg gcatcataga gagttgcttc
      721 ggaagttgct ccggacgaga attcccgagt aaccgggttt tctccactcc cggcaagtcc
      781 atcggaattc tgatcaagaa tttcttcgaa gagaattcct aaacctgatg tattgaagta
      841 ggagacacgt gcaatgagag ccgttcgggt tgagctgacc gtcttcgaag actgaaagat
      901 tcgcgcgcca accgtaatgc gcaccgaata ggtgccatcg acgaggccgg tgaagacaac
      961 gccggctgcg cccatgtctt gcgggccaga aacgaggacg ccgttctgat acaacgacac
     1021 tttcgcggtt gccggcacgg ccgctgccgc ggcgcgaata tcgcctctga tattgccacc
     1081 gccaatggcg ttgcgtacgg cgatctggtt gatgccacca ccgccccagt cgcctccccc
     1141 accgccgcca ccgcagccga cgcagaaaga gaccagaact accgccagaa agacgaccag
     1201 gggtcgcaga agatgtcgca tgagaagacc tcgcttcagg aaaatcgtgt accctgaaat
     1261 gatagaccag ccgctaccga aaggcaacga cgttctgcgg aaagttctct tcaggcgagg
     1321 gtctttccca tccgtactcg cactattaac ggagcattta aagcctgtgt cagcgtttga
     1381 ccgtgggttg cacaatggtc acacggcact tccttgctgc cttcaacaga gcggagtcaa
     1441 gcgttgccag tcgtgccgaa cccaagacct cacttgtgcc cttgcccaac gctaagcgaa
     1501 gaagccgcga cccagcgaga gggcggcttc cataaggtca gggtgtttgt cggccatggg
     1561 ttttcggccc tggtcaaggt caggcgcgat gaggcggtcg tacatttcga accccatcag
     1621 ggtgaacatc gcctcgaaca ttcggatagg actgagaaac tgccgcgcat ccgggtgact
     1681 ctgaacgtag agaaaaccca cacgctttcc gttcgcctca cgcggtgcaa agcccggatc
     1741 gaccagaggg tacaaacggt cgagacaggc tttcaccagt gccggcacat acccgaagta
     1801 aatgggactg gcgatcaaca gggcatcact gcgatcgatc aactcgtaca cccggctcat
     1861 gtcatcctgc tggacgcacc agcgcgtgat cggctcagcc ttgcacgcat tgcagctctg
     1921 acaggtattg atgcgcagat cgttgagata gagccactcg acctcgcccc ccttctcgat
     1981 cgcaccgcgg gcggcttctt cggcgagaat gcgggtgttg ctcgtcttgc gcgggctgcc
     2041 gaacaagatc gtcagtcgtt tcatgtgatg tgccccctct tgtcagcgag ttgtcgacag
     2101 cataccatgg gccgacatcc cacgcctgtg cgagattttc gacgagtcga acggaggaac
     2161 catgttctgg atctgggtcg cagtcatcgc ggtgattatc gtcatttcca agtgcttctc
     2221 gctcttcgtc tttccgcgca ttctgtcgcg atggtttacc ctgcatcacg aactttcgac
     2281 cgaagacggc tacgtcgccg tgcccgatct ttcgcatctc gtcggaaaac agggagtggc
     2341 cgtcaccgat ctcaaaccct gcggcaagat ccgtctcgac gacaccacct acgatgcccg
     2401 cgtcgagaaa ggcgtcattt cgaggggggc aaagatcgtg gtttcgcacg cacaggggaa
     2461 ctcgatcatc gtacaacctg ctgagatatg agatgagctt tctcacaaca ttctgagccg
     2521 gtgaccctcg accgaaaacc cgcctgacag aacaaacccg cgcggcaggc cgcgcgggtt
     2581 tttgtcgaaa tgcgatcgcc gagattacgg gcgcccggtg atcagaatgt aatgacgggc
     2641 atattcccgc atggcagccg agatctgctc gggcttcacc tgtttgaacg cttcgggcag
     2701 tttttcgaag aagtctgcct ggtagccttg agccaggaac ccggccaggg tcagagcgtg
     2761 ataccacgat gattccatct tctgcaggaa catgttcggc accaccttgc cggcgatctc
     2821 aacctgctcg gccgtgatct gggtttcggg cacctggcgc agaatgcggc gcagttcctc
     2881 gcggcattgt tccagtttgt caggggaaac ccgcaccaga aagtacaggt agctggcccg
     2941 atgcgtgtga cggaagacca catcctcgac ggcttcagcc aagcccttct gtacaagttc
     3001 tctctgcagc aaactgtctt cacttctggc aagatagtgc atcagcgagc cgagcgtgat
     3061 gaaatcgagc ggaatcggat catgccgcga agcgcgggcc ggcggcaacc ggtatcctcc
     3121 ggccaggtag gccgagccgg ccggcagacg cacctcaacc tcactggttt tttccagacc
     3181 ggccagtaga gccccatcgc caccggaccg cgtcggacct tcggtcgagc tcagatcgcc
     3241 aaacaccgcg cgcgcctggc gcagcccgtc agccgggtcg agatttccca ccaccgtgca
     3301 caggacattc ccgggcttca cccaggtctt caagaagtct cgcgcctcgg tcagtttcgt
     3361 cgccgcgaca ttctcgggcg gaacctgcga gtgcttccat tcgtcgccgg ggaatgcgag
     3421 tgccgtgaac ttttcgagca tgagcacgga agcctgcccg agcaacgtct cacgctcaga
     3481 tttcactttg gcggccaggc gggccagcag cttgtcatca aacaccggcg caaacacctg
     3541 cgacttcaga cactgcagca gagcttcata ctgatcaact ccggcgtagc cgacgacata
     3601 caacccgctg ccgggaatga cctgacagtg aaccaggccg ccgatctcat cgagacgtcg
     3661 cgccagttgt gtgtcacggg cttcgggccc agcctgggct tcgatcatgc gaaacaccag
     3721 ttcgggcagc cccttcatgg gcttgcggaa agaaccaccg ggctcaccga cacgcagaca
     3781 aacggccacg acgtcactgc caccgtacgg tttcacgacc acctgcatgc cgttgtccag
     3841 gttggccgac tgcacgaccg cgttctgacg cgacttcgcc gagatcggat gcgcgattcg
     3901 aacggcacag aggccattcg tgaacatgcg gcgggcaacg cgggcaagat catcggccga
     3961 cacgttttca atcgccgacc agatggaaga ctcaagcgaa aagtcaccgg caagttcgcc
     4021 gatgccgagc gagattccct gcacagcccg gaactccgac ttcagactgc gaatactctt
     4081 gcagttcgcc aagatgcgct gcagttcggc atcgcgcggc ggtacacgcc agagagccga
     4141 gagcttgcgc aacaaccccg acaccacgac atcgacgtcg acatcggggc tctcaaacga
     4201 aatcaggaag gtccccttgc ccacatacgg ctggtactga gcactcaggt tcgacagttt
     4261 ggggaagtcg tgccgattga cacgcccaag ccaggaatca ttgacgtggg acatggcgat
     4321 caaccagaca ctggcggccg cacaatcagg atcatcgaga gccggtgccg gaaacccgat
     4381 gaccacctgc gtattcttga tatcgagata ctcgcgcttc tcgacgaccg aagccgtgca
     4441 gacggcatcg ggttgaacga tcgacttctc gtcggcacgt tcgcgatacg cgggcgctgg
     4501 ttttgcctcg gcgaccggca acgcggtcag atcaccataa acgggcatga gacccttgaa
     4561 gatctcgcag gccataacgt cgccaaccac gaccagagcc gcattgttgc tgacgcactg
     4621 ttcctgatac atcttctcga cgtcggccag agtgagtttc ccgacttcct cctgtgtcgc
     4681 gaagaaacgc gcccccatcg gcaacccttc gaacagcatg ggagcaatcg catcagaaag
     4741 atatccggaa gacaacggaa actgcagacg ctgtcgcgct ttctgcagtt cgagattctt
     4801 ggcccggtcg aacgcttcac gggaaaacgt ctgcccaaag gcgaggcgac gcagacttcg
     4861 aagcgccacg tcgagactgt ctgacgggcc ctggatcatg agaatcaggc cctgagcggt
     4921 gtcaaccacc tgcggcggat ggcacccctg cttggccagc tccgtcagaa acgcggaacg
     4981 ggtctcgccg accggcgcag ccgtttcgag cagtcgagcc agaacagcga ccaggccggg
     5041 agcaaccgga cgcgctacgc cgggcccgac cttgaacagc agcttgcacg agaccagcgg
     5101 cagactgtgg tcttcctgaa ccaccacccg caggccgttg ggcagaatgg cccgctgcgg
     5161 ctgaggcttg atcagggccg atgcctggct tgccagggcc agcacacaga ccagaaggct
     5221 gataacgaaa cggatgtgaa cgcgagacat gcgattcctc ccccgcgaag agtcgattat
     5281 tccttactga acacgacccg cccctgggac agatcgcgta tgatgacaac gtaactgcgc
     5341 acgaccacca tcttatagtt gtcggcgcca tagatctcaa cccggtaccc cgcgcgatcg
     5401 gcaatcgcct tgcgcgcttc gagcagaaac ggctgaatgc cggtatgcac gttttcggtg
     5461 atgcggcgat attcgcggtc atcgatgccg atctcgaacg gatattcctc gtagcgatcg
     5521 gtggtgatgc gcaactcgag cttgtacgac accatcttcg ccgaggcagc gaacagcgat
     5581 gccaacagca gaaacgccga caacaggtac accgttttac gcataccggg catggtagac
     5641 gcgagccggc aacctgtcaa caaaatcaga cagtcctatg atcctgttgg tgacggggat
     5701 cagacgggtt cgccggaaag ggtccagagg cgggcctggg tgatgcgcac ccggcgcatc
     5761 gtgccgatct gttctgccgt gccggcgaaa tcgacactgc gaccgtgcgt agtcttgccc
     5821 ttcacgcggc cgggc
//
LOCUS       DAORHD010000104         5820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_13957_length_5820_cov_3.590286, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000104 DAORHD010000000
VERSION     DAORHD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13957_length_5820_cov_3.590286"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5820)
                     /locus_tag="PKO06_03450"
     CDS             complement(<1..>5820)
                     /locus_tag="PKO06_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68727.1"
                     /translation="LSESRTEVLKQLIPGTEDHYYYSCLNEQNRGNFSQARALLEQWI
                     KAQGLTARAREIQYRQTLLEYPTRPEESLSFLKNELNLRFEHQRRQQTPKTSYPSELD
                     PSLISRQSLTRQAFSLYSNLDGFEISAWPFLAREPLDGHKRRHFLTRLQRPDIPGLVE
                     MIVADLQTEDNGGFGYLSIHSQLLPQQLEQCLGRLPELGEDTNFIQTWLSKLQPSPDQ
                     DWKNDPKIRQAHLERLWNFVRKLSPNQNSLKVHVLYQQLILDRQLGTYDAKKFLEYLK
                     LPRSASYVNREYLVRKEHRDHQANLHQDFGRFTLFPPVVDDEPLVRDYLETFLKDAES
                     PNEYAPYFDKDYLKRVFAETKLQEGTGKAENLLALLSADSVKNLKEQILLEFLPSNPQ
                     VIGMNDPLALKVRVKNIPNLIVKVFRINTFNYYRQQQQEISTSIDLDGLVANEEKVLT
                     YAQPDIRRHTELYEFPTLKEPGLYVIELIGNGRSSRALIRRGRLTFTERSGSAGQVFT
                     VYDEAGVPVKDASIWMSGHEYTPDERGQINIPFSTNPGTQPIIIRRGEVAVLHQMTHQ
                     AENYSLQARLFVDRESLLERRQATLIVRPWLTVNGTPIDIKLLEEVRVRIDSTDRDGI
                     SSTREIPAPELQNATETLIPFKVPEKLSHISFTLRGKVQNLSQNKKIDLEVSQGYTLN
                     GIEKTDKIDDLHLRLVDGRYLLELRGKTGETKPDRVVTVEVKHRDFVRTVKLSLQTND
                     QGRIYLGSLNDIVWIKAEGPQGVSEHWELLRDRALLPGVIHARVGETVHLPMPYAVDT
                     APAELFSLLEVRGSTYVKNCAEFGKVQNGQLSITGLTGGDYDLFLLPEQRGIRLRVTE
                     GQLNEGWAQSPARTLEMRVQTPLALEAPTVDQARGKIVVKVHNVNAAARVHLVATRYL
                     PGTSVFDGLQTFTRPDPREYRLNPSVSQYVSGRNIGEEYQYILDRKYAKVRVGNLLRQ
                     PSLLLNPWNLRKTDTTRQDAAGGESWQNMPVPCPAMEASTGGYAGDAPGEPEPVEAGG
                     FASLDFLPSTSLLIANLRPNAQGLVEVDLSALEGRQHVQLLAIDQHSAICRQLSLPET
                     AYTPKDLTIAQPLEPTQHFTEQKSISTVASGSEFTLADITTAKLEMYDSLQSVYGLFS
                     TLSSNPTLAEFSFILEWPELSAARKGELYSKYACHELSFFLYHKDPEFFQKVIKPYLA
                     NKRDRTFMDDWLLENDLTSYLTPWRYGRLNTVERILLARRLPNEFDSTARHIQDRLDL
                     LPPDLERWNQLFNTALKGSSMDTDDRLGFDKAQAAAPAKPRPMAPPPPPAPASVAPMG
                     LVAPTAAPRQSSKGARNELMKAKKDMRSEEKVAREAASFADAEGMDLDEASAVDDFKE
                     QEEDSGRRRVQRQLYQALDRTEEWVENNYYRLPIEKQVADLITVNAFWRDYATHKAGT
                     PFLSKNVAEAARSFPEMMLALAVIDLPFKPGSHNVSYTEARMSLKAVQPAVFFHKEIR
                     ETGAAGKVQEILTGQNFFAADDRYRYENSERLDQFVTEEFQTWRVYGCQVIITNPSST
                     RRKVDALLQIPQGSVPVNNGFYTRSINLQLDPYTTKTVEYYFYFPKAGRYTHFPIHIS
                     QESRLLAAAEPFVFQVVDQLTKIDKTSWPWISQEGSMEDVLAFLENNNVDRLDLNLIA
                     FRLRDRSFFTKVIALLQKRHVYHETTWSYGLYHNEPAVIREYLAHASWANQCGLYLDT
                     PLLTLNPVWRHAYQHREYWPMVNARAHQLGKDRKILNRQFHEQYLLFLRYLQYRQALD
                     DGDRLGVVYYLLLQDRIDEALSWFSSVKNQEVCSSIQYEYLKAWLAFSEERAAEGLAI
                     AERYKDYPVDRWRKLFQEMLQQADEIAGKGPVVIDQEDRNQVQAHLAATEPNLELKVE
                     GGRLQLAHQNLKGCRVNFYQMDLELLFSRNPFVQGVSGQF"
BASE COUNT         1138 a         1605 c         1780 g         1297 t
ORIGIN      
        1 gaactgaccc gacaccccct gcacgaaggg gttgcgcgag aacagcagtt cgagatccat
       61 ctggtagaag ttcacgcggc accctttgag attctggtgt gcgagttgca gacgcccgcc
      121 ttcgactttc agctccaggt tgggctcggt cgcggccaga tgtgcctgta cctggttgcg
      181 gtcttcctgg tcgatgacga ccgggccctt gccggcgatt tcgtcagcct gctgcagcat
      241 ttcctgaaac agcttgcgcc agcggtcaac cgggtagtcc ttgtaccgtt cggcaatcgc
      301 cagaccctct gcggcccgct cttcactgaa cgccaaccag gctttcaggt attcatactg
      361 tatcgacgag cacacctcct ggtttttcac ggaactgaac caggacaatg cttcatcgat
      421 gcgatcctgc agcagcagat agtagacgac tcccagacga tcaccatcgt ccaatgcttg
      481 tcgatactgc agatagcgca ggaacaacaa gtactgttcg tggaactgac ggttcaggat
      541 cttgcgatct ttgcccagct gatgcgcccg cgcgttgacc atcggccagt attcgcgatg
      601 ctgataggca tgacgccaca ccgggttgag cgtcagcaac ggggtgtcga gatacaaccc
      661 gcactgattg gcccacgatg cgtgcgccag atactcacga atgaccgcgg gctcattgtg
      721 atacaggccg tatgaccagg tagtctcgtg atacacatgc cgcttctgca acaacgcgat
      781 gaccttggtg aagaacgatc gatcgcgcag gcggaaggca atgaggttca gatcgaggcg
      841 atcgacattg ttgttttcca ggaacgcgag aacgtcttcc atgctgcctt cctgagagat
      901 ccagggccag gaggtcttgt cgatcttggt cagctggtcg accacctgaa acacgaacgg
      961 ctcggccgcg gcgagcaagc gagattcctg cgagatatga atcgggaagt gcgtgtaccg
     1021 gccggccttc gggaagtaga agtaatattc gacggtcttg gtcgtatacg ggtcgagttg
     1081 gagattgatg ctgcgcgtgt agaacccgtt gttgactggg acggacccct gcggaatctg
     1141 caacagcgca tccaccttcc ggcgcgtgga actggggttg gtgatgatga cctgacaccc
     1201 gtagacccgc catgtctgga actcttcggt gacgaactga tcgaggcgtt cgctgttctc
     1261 ataccgatag cgatcgtccg cggcgaagaa gttctgaccg gtaagaatct cctggacttt
     1321 gcccgcggca ccggtctccc ggatttcctt gtggaagaac acggccggct gaacggcttt
     1381 cagcgacatg cgggcttcgg tataggagac gttgtggctt cccggcttga aaggcagatc
     1441 aatgacggct aacgcgagca tcatttcggg gaagctgcga gctgcctcag ccacattctt
     1501 cgacaggaac ggagtaccgg ctttgtgagt cgcataatcg cgccagaagg cattgaccgt
     1561 gatcagatca gcaacctgct tttcgatcgg tagccgatag tagttgtttt ccacccattc
     1621 ttccgtgcga tcgagagcct gatacagctg ccgttgcacg cggcggcggc cgctgtcctc
     1681 ttcctgttcc ttgaaatcgt cgaccgccga agcctcgtcg aggtccatcc cctctgcgtc
     1741 ggcgaacgaa gcggcttctc tggctacctt ctcttccgaa cgcatgtcct tcttggcttt
     1801 catgagctcg ttgcgagctc ccttgctcga ctgacgcgga gcggccgttg gggccaccaa
     1861 ccccatgggt gctaccgagg cgggggccgg cggtggcggc ggagccatcg gtctcggttt
     1921 ggcaggggct gcggcctgtg ccttgtcgaa cccgagccga tcgtcggtat ccatcgaact
     1981 gcccttcagg gcggtgttga acaactggtt ccagcgctcg agatccgggg gcagcagatc
     2041 aagacgatct tggatgtggc gggccgtcga gtcgaattcg ttcggcagtc ggcgcgcgag
     2101 cagaatgcgc tcgacggtgt tcagtcggcc ataccgccag ggagtcagat agctggtcag
     2161 atcgttctcg agcagccagt catccatgaa cgtacgatcg cgcttattcg ccaggtaagg
     2221 tttgatgacc ttctggaaga attctggatc cttgtgatac aggaaaaagc tcagttcgtg
     2281 acacgcatat ttcgaataga gctcaccttt gcgtgctgcg gacagttcag gccattccag
     2341 aatgaacgag aattcggcca gggttgggtt gctcgacagc gtcgaaaaca gaccatacac
     2401 gctttgcagc gagtcataca tttccagttt ggcggtcgtg atatcggcga gggtgaactc
     2461 gctgccggaa gcgacggtcg agatactctt ctgctcggtg aagtgctggg tgggctcgag
     2521 cggttgggcg atcgtcaggt ccttgggcgt gtaggccgtc tccgggagcg acaactggcg
     2581 acagatcgcc gagtgctggt cgatcgccag cagctgcacg tgttgccgtc cttcgagcgc
     2641 gctcagatcg acttcgacga ggccctgggc attcgggcgc agattcgcga tcagcagcga
     2701 ggtggaaggc agaaagtcca ggctggcgaa accgccggct tcgaccggtt ccggttcgcc
     2761 gggcgcatcg ccggcatacc cgccggtgct ggcttccatg gccggacacg gcacaggcat
     2821 gttctgccac gattcaccac ctgccgcatc ctgtcttgtg gtgtcggtct tgcgaagatt
     2881 ccagggattg agcaacagag agggctgacg caacagattg ccgactcgta cttttgcata
     2941 cttgcgatcg agaatatact gatattcctc gccgatgttg cgaccggaaa cgtactggct
     3001 gacggagggg ttaagccggt attcacgagg atccggtctg gtgaaagtct gcagaccgtc
     3061 gaagacgctg gttcccggca gatatcgggt cgcaaccaga tgcacccggg cggcggcatt
     3121 cacattatgc accttgacta cgatttttcc cctggcctga tcaacggtcg gcgcttccaa
     3181 cgccagaggg gtctgaacgc gcatttcgag cgtgcgcgcg ggtgactggg cccagccttc
     3241 gttcaactga ccctcggtga cccgcaggcg aatcccacgc tgttccggca ggagaaacag
     3301 gtcatagtca ccgccggtga ggcccgtgat ggaaagctgt ccgttctgca ccttgccgaa
     3361 ttcggcacag tttttgacat acgtgctgcc acgcacttcg agcagcgaaa acagctcggc
     3421 gggagcggtg tcgaccgcgt agggcatggg cagatgcacg gtttcgccga ctcgcgcatg
     3481 gatgacgccg ggcagaagcg ctcgatcacg caacagttcc caatgctcgc tgacgccttg
     3541 cggaccctcg gccttgatcc agacgatatc attcagggag ccgaggtaga tgcgcccttg
     3601 atcgttggtc tgcaggctga gtttcacggt gcgaacaaag tcacgatgct tgacttccac
     3661 ggtgaccaca cggtcgggct tggtctctcc ggttttgccg cgaagctcaa gcagataccg
     3721 accgtcgacc agacgcagat gcagatcatc gatcttgtca gtcttctcga tgccgttcaa
     3781 cgtatagcct tgggaaacct cgaggtcgat tttcttgttc tggctgagat tctggacttt
     3841 gccgcgcagc gtaaaactga tgtggctcag cttctccgga accttgaacg ggatgagggt
     3901 ctcggtggca ttctgcagtt ccggggcggg aatctcgcgc gtggagctga tgccgtcacg
     3961 atcggtcgaa tcgatgcgca cgcgtacctc ttccaacagc ttgatgtcga tcggggtgcc
     4021 gttgacggtg agccacggcc tgacgatgag cgtcgcctgc cggcgctcga gcagactctc
     4081 gcgatcgacg aacagacggg cctgcagaga gtagttctcg gcctgatgtg tcatctgatg
     4141 cagcacggcg acttccccac gacgaatgat gatgggctga gtgccggggt tggtactgaa
     4201 gggaatattg atttgtccgc gctcatccgg cgtgtattca tggcctgaca tccagatcga
     4261 ggcgtctttg acggggacac cggcttcatc atagacggtg aatacctgac cggcagagcc
     4321 tgaacgctcg gtgaaggtca gtcgaccgcg acgaatcagg gcgcgactcg atcgtccgtt
     4381 tccgatcagt tcaatcacat acagccccgg ctctttcagg gttgggaact cgtacagttc
     4441 cgtgtgtcgt cgaatatccg gttgtgcgta ggtgagcact ttctcttcgt ttgccaccag
     4501 accgtccagg tcgatgctcg tggaaatctc ctgctgctgc tgacgatagt agttgaaggt
     4561 gttgatgcgg aagaccttga cgatcaggtt ggggatgttc ttcacccgca ccttcagcgc
     4621 caacgggtcg ttcatcccga tgacctgcgg attcgatggc aggaactcga gaagaatctg
     4681 ttccttgagg ttcttgacgc tatccgcgga aagcagcgcg agaaggttct ccgcctttcc
     4741 ggttccttcc tgcaacttgg tttcggcgaa gacgcgcttc agataatctt tgtcgaagta
     4801 cggcgcatac tcgttcggtg attccgcgtc cttcaggaag gtctcgaggt agtcgcgtac
     4861 cagcggttca tcgtccacga cgggcggaaa cagggtgaaa cggccgaaat cctgatgcag
     4921 attggcctga tggtcacgat gttccttgcg gaccagatac tcgcgattga cgtagctggc
     4981 cgatctcggc agcttgaggt attcgagaaa cttcttggcg tcgtaggtgc cgagctgccg
     5041 gtcgagaatc aactgctgat acaggacatg gactttcagc gaattctgat tcggactgag
     5101 tttcctgacg aagttccaca ggcgttcgag gtgtgcctga cggatcttgg gatcgttctt
     5161 ccagtcctgg tctggtgacg gctgcagctt ggagagccag gtttgaatga agttggtgtc
     5221 ctcgcccagt tcgggcagtc gcccgagaca ctgttccagt tgctggggaa ggagctggct
     5281 gtgaatggac agatacccga atcccccgtt gtcctccgtc tgcagatcag ccacgatcat
     5341 ttccaccaga ccgggaatgt cggggcgttg caggcgggtc aggaaatggc ggcgtttgtg
     5401 accgtcgaga ggttcgcgcg ccaggaacgg ccaggcagaa atctcgaacc catcgagatt
     5461 cgagtagagt gaaaacgcct gccgtgtgag gctctgacgg ctgatcagag agggatccag
     5521 ctcggacgga taggaagtct tcggcgtctg ctggcggcgc tgatgctcga accgcagatt
     5581 cagctcgttc ttcaggaacg acagagattc ttcgggccgg gtcgggtatt ccagcagggt
     5641 ctgacgatac tgaatttcac gggcgcgcgc ggtcagcccc tgagctttga tccattgttc
     5701 cagcagggcg cgtgcctggc tgaaattgcc tcggttttgc tcgttcagac acgagtagta
     5761 gtagtggtct tcggtgccgg gaatgagctg cttcagaacc tcagtgcgtg attcgctgag
//
LOCUS       DAORHD010000105         5782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14101_length_5782_cov_4.017810, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000105 DAORHD010000000
VERSION     DAORHD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14101_length_5782_cov_4.017810"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..>5782
                     /locus_tag="PKO06_03455"
     CDS             90..>5782
                     /locus_tag="PKO06_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV68728.1"
                     /translation="MSDPVPRVARESLLRQAVSAWWMPFVLSLLPLLILLFAGREEAE
                     REYKSRVDDLRNQCERISRSLPQATDIEYWLQTIAGRLRRYLLRTPRAGIDAGAIRRT
                     FPAGMEQTRFWIFRQEDRLRPVTGKGFEIEFADQFARLLQELSLSEGRGTDKQMGTAK
                     AGIDAKLLQRWRMLLGTGLTAELFASSFRGQPFHVIFQNQPSLMLWDLITDKGTVRAG
                     FVCIFPIADMTPERPLLVCMKNWSHPRIRPGFLPLPYTEGPILELPLLHPDLCRPAVM
                     QAVLKLQERMGIFPADGADLFHKDIRVLSPLIGRLLPLTERYWAFPTPVSPVSGHLGL
                     LVTPALQPRLSSLERLAHLLLFLWGACWIVTVGQLLIRQRIPAPGVHIELVILFLALV
                     SMPLMLSYSNSTRLAADVTANRLDERRAAINRIMSNIEAGTVQVNQSICRTVRGFWDD
                     QHLRERLLAADQAGPLLRELNGRFREQGQVIAGILLFRRDREEPDHLDLSGVTAGSQK
                     LLIRILTREWKNSIGEAATAGSNTDDIITRDASTWGRRPGFFGQTRELGSLRAWRLGE
                     RQWFMYSIVWKRDNKAMMQLDCFWRLEDHLERYLVDAFDEQLRISQEEDLGLAAYRIR
                     GNRVFESAAATGVVAGRPEFSRLAATAVFKPLQLERPEQGDLVIARQSRHLPGYVLLA
                     RASTQIVQERNRQLLTSMTLLLLSLLTPAFLISRWLRKRLAEPIVALAEVLEEVGQGN
                     LDRQVQVSRQDELGQAARSVNTMIAWLRERRMLSRFVAPQVLDVVAGGDYRRAMEGDH
                     RQVAVLVSDIRSFTTLSETYPATAIFELLNRHMERMTRAIQREGGVIDRFVGDAIQAV
                     FYERVGAISPAIRALRAAAAMKREHQAINREREQQGSFAYQIGIGVDVGTVVAGVIGD
                     PGSRLDFSVLGEPLRRAGELEAASKQAQYTGIMVSPDVCLAAEEAFSFTPCQGAPDVM
                     ELRAIPQLEEIDDLARGNASAETLRPRTISAEEPVDDPASADLSAPVLSPGLLAFLWG
                     LPLICFLAVWLWGGTRLVESQRSMVRQALDETLGIHENLFAVLPQAGMWLKSRLQQLP
                     TQPFSFEAWGRCWQEFRQIAPRASFIALQQGKPLPFRDALEAHRDFYDEQFFRVASAS
                     VSPGFPLVASDALRFWACLAFHDLHYPSAGEEDRLSILRPSLFQWRLNEVEKSDRFAG
                     IAGHTFEELVREGLGAFYPATIDWHPGAFFWLPMREIGRPSMSPAEKIDRTTGLMVFL
                     PVEHMTPEVGYRFFLQTLRQSGLEGAVVWRQGSQWKSLLSDDFFGKKLLDALSQRLQN
                     LENVGGAGLMGYQDLEDGALAWRQPSGDSQVLIMVGQRSQRIQKPLWLEAQTSGLIVG
                     GWILLGVIWLTGGRMRRLLRRSLLAQMIMGFALVILPSLGIGFQLLETYIDERSMRAE
                     KEQGERLIADLESMDRGHELWTAYACRLYERFLSDPGQLEEFRREMDQADPDAFRGND
                     LFWERLVKGAEWRGLLISNLMVAGTGRYVRGFMQDSARAEAYRALFEKNLGKLNPDAD
                     TVAREGKRESEKRSTMIGAQVDDFVESMQTMVPAQVFAGQLAAPRLLVRLMLQDQVEQ
                     LFYMQHLRLDGFSKAIMQIVFDDKCFMKLLFREWQMMNTALGDSSAFEVSYGNAPGWK
                     FIPPFWRTYYRFEKSLNPAYADIRRGMFLHRQRLQDHARANVAAEAFAAGQPRVRRIR
                     TAEGEVLQAAVPGTVNSRLVYSHGVALDTVQSNALAKIRRERLLLILLAGIALILAVR
                     ASRRFLAPLLELERCARRVTQLDFTARMNIERDDELGRLAVAFNRMTEGVQQGRLLRQ
                     FVSDSVQVMVGEKAHSERKASGEQVELVVLFTGVAEFKTLLKTLPADRLIRGLNE"
BASE COUNT         1077 a         1523 c         1885 g         1297 t
ORIGIN      
        1 gaacggccga gcgtgggtcg cagggccgtg gtcgcaggcc gccgcggcga taaacgtccg
       61 tattgtcgcc ttggacgagc actgaggcga tgagcgatcc tgtaccgcgc gtcgcgcgcg
      121 aaagtttgct tcggcaggcc gtgagtgcct ggtggatgcc attcgtcctg tcactgttgc
      181 cactgctgat tctcctgttc gctgggcgtg aagaagcgga acgtgagtac aagtcgcgtg
      241 tcgatgatct tcgcaaccag tgcgaacgaa tcagtcgcag tttgcctcag gccacggata
      301 tcgaatactg gcttcagacc attgccggcc gtctgcggcg ctatctgtta cgcacgccgc
      361 gtgccggaat cgatgccgga gccattcgtc gcacctttcc tgccggcatg gaacagacac
      421 ggttctggat ctttcgtcag gaagaccgcc tgcggccggt gaccggcaaa gggtttgaga
      481 ttgagtttgc tgatcagttc gccaggttac tgcaggaatt gtcgttgtcc gaggggcgag
      541 ggactgacaa gcagatggga acggcgaaag cgggcattga tgccaaactg ctgcaacgct
      601 ggcggatgct gctgggcact ggtctgacgg cggaactctt cgcgtcatcg ttccgtgggc
      661 aaccgtttca cgtcattttt cagaaccaac catctctgat gttgtgggac ctcatcaccg
      721 acaaagggac ggtgcgagct gggttcgtct gtatttttcc gatcgcggac atgacgccgg
      781 aacggccgct cctggtctgt atgaagaact ggagtcatcc gaggatacga cccgggttcc
      841 tcccgctgcc gtacaccgaa ggcccgattc tggaactgcc gctgctgcat ccggacttgt
      901 gtcggccggc ggtcatgcag gcggtgctca agctgcagga acggatgggc attttcccgg
      961 cagatggagc ggatctgttc cacaaagaca ttcgggtgct cagtccgttg atcggacgcc
     1021 ttctgccgct gacggagcga tactgggctt tcccgacgcc tgtatccccc gtttccggac
     1081 acctgggcct gctggtgacg ccggcgctgc aaccgagatt gtcttcgctc gaacgactgg
     1141 ctcacttact gctgtttctg tggggggcgt gctggatcgt cactgttggc cagttgctga
     1201 ttcgccaacg gattcccgcg cccggagtgc atatcgagtt agtcatcctg tttctggcgc
     1261 tggtttcgat gcctttgatg ctgagctaca gcaacagcac gcgtctggcg gctgacgtga
     1321 ccgcgaatcg tctcgatgag cgccgcgcgg cgataaatcg catcatgagt aatatcgaag
     1381 ccggtacggt gcaggtgaat cagtcgattt gtcgcacggt tcgcggtttc tgggatgatc
     1441 agcacctgcg agagcgcctt ctggcagcgg atcaggcggg accgctcctg cgtgaactga
     1501 acggacgttt tcgggagcag gggcaggtga ttgccggcat tctgctgttc aggcgcgatc
     1561 gtgaggaacc cgaccatctg gatctttcgg gggttacggc cggatcgcag aagctgctga
     1621 tcagaatcct gacgagagaa tggaagaact cgatcggcga ggcggcaacc gcgggttcga
     1681 acacggacga tatcatcacc cgagacgcat cgacctgggg acgaaggccc ggctttttcg
     1741 gtcagacgcg tgaacttggg tcgttacgtg cgtggcgttt gggagagcga caatggttca
     1801 tgtattccat cgtctggaag cgtgacaata aggcaatgat gcaactcgat tgcttctgga
     1861 gactggaaga ccatctcgaa cgctatctgg tcgatgcctt tgacgaacag ctgagaatca
     1921 gtcaggaaga ggatctcggc ctcgccgcgt atcgcattcg gggtaatcga gttttcgagt
     1981 cggccgcggc gacgggagtg gtggcggggc gaccggagtt ttcgcgtctc gccgccacgg
     2041 cggtgttcaa acctcttcaa ctcgaacgac ccgaacaggg tgatctggtg attgcgcgtc
     2101 agagtcggca cctgcccggg tatgttctgc tggcgcgagc ttcgacgcag atcgtgcagg
     2161 agcgcaaccg gcagctgttg acgagtatga cgctgttgct gctcagtctt ctgaccccgg
     2221 catttctgat ctcgcgatgg ctgagaaagc gtctggcgga acccatcgtc gccctggccg
     2281 aggtgctcga agaggttggc cagggcaatc tcgatcgtca ggtgcaggtt tcacgtcagg
     2341 atgaactggg acaggcggcc cgatcggtga acacgatgat tgcctggctc cgcgagcggc
     2401 gcatgcttag tcggtttgtc gcgccacagg tgctcgatgt cgtggctgga ggagactacc
     2461 ggcgggccat ggagggcgat caccgccagg tggcggttct ggtgtcagac attcgaagtt
     2521 tcacgacgtt gagcgaaacg tatccggcga ccgcgatttt cgaactgctg aatcgacaca
     2581 tggagcggat gacacgggcg attcagcgcg agggaggagt catcgatcgg ttcgtcggcg
     2641 acgcgattca ggcagtgttt tacgagagag tcggcgcgat ctcacccgcg attcgggcgt
     2701 tacgtgcggc tgctgccatg aagcgagaac atcaggccat caaccgcgaa cgtgagcaac
     2761 agggcagctt tgcctatcag atcggcatcg gtgtggatgt cggcacggtc gtggccggcg
     2821 tgatcggaga tccgggttcg cgtctcgatt tttcggttct gggcgagcct ctgcgccgcg
     2881 ccggagagct tgaggctgct tcgaaacagg cccagtacac gggaatcatg gtctcgcccg
     2941 acgtctgcct ggcggccgaa gaggcgttct ccttcactcc ctgccagggt gcccctgacg
     3001 tgatggagtt gagggcgatt ccccaacttg aggaaatcga tgatctggcg cggggaaacg
     3061 catcggcgga gactctccgg ccacgaacga tctccgcgga ggagcccgtg gacgatcccg
     3121 cgtctgctga tctgagcgca cctgtgctct cgccgggcct gctggcgttt ctctgggggc
     3181 tcccgttgat ctgctttctc gccgtctggc tgtggggcgg gactcgcctc gtcgagtcgc
     3241 agcgttcgat ggtgcgacaa gcgctcgatg agactctcgg aattcatgaa aacctgttcg
     3301 ccgttctgcc gcaggcggga atgtggttga aatctcgttt gcagcagctt ccgacgcagc
     3361 cgttttcctt cgaagcctgg ggtcgctgtt ggcaggagtt tcgccagatc gctccccggg
     3421 catcttttat tgccttgcag cagggaaaac ctctgccgtt tcgcgatgct ctcgaagctc
     3481 accgggattt ctacgatgag cagttctttc gtgtcgcgtc ggccagtgtc tcgcccgggt
     3541 ttccgttggt tgccagtgac gcgctgagat tctgggcgtg tctcgccttt catgatctgc
     3601 attacccgag tgcgggcgaa gaagatcgcc tgtcgatact gcgcccttcg ttgtttcagt
     3661 ggcgcttgaa tgaagtcgag aagtctgatc ggtttgccgg tattgccggg cacaccttcg
     3721 aggaactcgt tcgtgaaggg ttgggggcat tttatccggc aaccatcgat tggcaccccg
     3781 gagcgttctt ctggctgccg atgcgagaaa tcggtcgccc gtcgatgtct ccggctgaaa
     3841 agatcgaccg tacgacgggc ctgatggtgt ttctcccggt cgaacacatg acgcctgaag
     3901 tcggctatcg gttctttttg cagacgcttc gtcagagcgg cttagaagga gccgttgtct
     3961 ggaggcaggg aagtcaatgg aaatcgttgt tgtctgacga cttcttcggc aagaagctgc
     4021 tcgatgcctt atcacagcgc ttgcaaaacc tggagaacgt tggaggggcc ggtttgatgg
     4081 gatatcagga ccttgaagac ggagctttgg cgtggcgcca gccgtctgga gattcccagg
     4141 tgctgatcat ggtcggacaa cggagccaaa gaattcagaa gcccctctgg ctcgaagcgc
     4201 agacgagtgg actgattgtg ggtggctgga ttctgctcgg ggtgatctgg ctgacaggtg
     4261 ggcgcatgcg gcggttgttg cgccggtctt tgctggcgca gatgatcatg ggttttgccc
     4321 tggtcatcct tccctcgctg ggtatcggtt ttcagctcct ggaaacatac attgatgagc
     4381 gttcgatgcg tgcggaaaag gagcagggcg agcgtcttat cgcagatctc gagagcatgg
     4441 atcgcggtca tgaactgtgg acggcgtatg cctgtcgtct gtatgaacga ttcctctcgg
     4501 acccggggca gttggaagag tttcgccgtg agatggatca ggcggacccg gatgcctttc
     4561 gtggcaatga ccttttctgg gagcggttgg tcaaaggagc ggagtggcgg ggactgctga
     4621 tcagtaatct catggtggcc ggtaccggaa ggtatgttcg tggcttcatg caggactcgg
     4681 cgcgggcgga ggcgtatcga gcgctgttcg agaagaacct gggcaaactt aatcccgatg
     4741 cggataccgt agcccgcgag ggaaaacgag aatcggaaaa gcgttcgacg atgatcggag
     4801 cccaggtcga tgactttgtc gagtcgatgc agaccatggt tccagcgcag gtgtttgccg
     4861 gacaactggc ggctccgcgg ctgttggtac gcctgatgct gcaggatcag gttgaacagc
     4921 ttttttacat gcagcatctg cgtctggacg gattttcgaa agccatcatg cagatcgtct
     4981 ttgatgacaa gtgcttcatg aagctgttgt ttcgcgagtg gcagatgatg aacacggcac
     5041 tgggagattc ctctgccttt gaagtgagct acggaaatgc cccgggctgg aagttcattc
     5101 cgccgttctg gagaacctac taccgcttcg agaaatcgtt gaatccggca tatgcagata
     5161 tacgcagagg gatgttcctc catcgacaac gactgcagga ccacgcgcgt gccaacgtgg
     5221 ccgcggaggc atttgcggcc ggacagccgc gagtccgtcg gatccgaacc gcggaaggag
     5281 aggtattgca ggcggctgtc ccgggcaccg tgaacagtcg cctggtctat tcgcacggag
     5341 tggctctgga tacggtgcag agcaacgcgc tggccaagat caggcgcgaa cgccttctgt
     5401 tgattctgtt ggcgggaatc gctctcattt tggcggttcg tgcttcccgc cgctttctgg
     5461 cgccgttact ggagctggaa cgctgcgcgc gccgggtgac gcagctcgat ttcaccgccc
     5521 gcatgaacat cgaaagagac gatgaactgg ggcggctggc ggtggctttc aaccggatga
     5581 ccgaaggcgt gcagcaggga aggttgctgc gtcagtttgt ttctgattca gtacaagtca
     5641 tggttggaga aaaagctcat tcagagagaa aggcgagtgg tgaacaagtc gaactggtcg
     5701 ttctctttac gggagtcgcc gagttcaaga ctctgctgaa gacgcttccg gctgatcgat
     5761 tgattcgggg actgaacgag ta
//
LOCUS       DAORHD010000106         5767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14164_length_5767_cov_4.306373, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000106 DAORHD010000000
VERSION     DAORHD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14164_length_5767_cov_4.306373"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1325)
                     /locus_tag="PKO06_03460"
     CDS             complement(<1..1325)
                     /locus_tag="PKO06_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV68729.1"
                     /translation="MTDTTIPTTPLPEPASRISLQARVFLAMSLLLLATLCGTSWVET
                     ERARESYERDIRSHVNGIWKSLDELVRPLLIERNVPLLTTIALKLTDRETADNPLQGM
                     AFLSRDMITLAHSHPASAPVDLESRRASDIIQFLRPIRPPERTEPLGYLRLEYSTMPL
                     GQKQDEILRAHLWLFVFLLFFAWMLAGVVARALKAPLRSLEETANQLSSGRLDARVPG
                     QPQEDLGSLVDSFNRMADSIGAHLKDLDQKNVSLERRVFELSTLQQAGRIINSVLNLD
                     RLYEVIVDTTIQVLGGVKRCSLMLADRPSNEFVIKIAKGLDVGNLPDNRRVPITDGVA
                     GKVFQTGEPILINDLAEGEKTLMLDPARISRSSICVPIKVNDDVIGIISAGNKMTGER
                     FTANDLALLETLATQAGIAMKNAKLYFDLDRKIIELGTLHEVGKSLSMVL"
     gene            complement(1322..2533)
                     /locus_tag="PKO06_03465"
     CDS             complement(1322..2533)
                     /locus_tag="PKO06_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68730.1"
                     /translation="MSIPLPGAGNDMEKLQTNITLGIGSNYFSIAGVLAAWVVLMKTG
                     WRDGFWETIFASMAVAPAMMGDALDSYFLNRVRVDTEKGWNDVFITEAGRNHLTKFHV
                     WHRLHSLLSAGFLAGILLVGFSSDPSIYQWFRPVFGLLFLTLSLRCLFVQIRYLAPAL
                     TSRRVPAFGKRWVFVLALGALWFWWLYARDPGKPFSPMGMALHGLIYFFLCGILHPLP
                     SRYSILHLDRNPGPRLPREIKVFPDGTPNPLLETDMKDQLPLWQNAGFTPLSVFSMPL
                     LEMPIFQTVGQAWIGPERRVLLLLQVSEGRENAHRTLVSWRGDQILISSDFGAATARF
                     PEGIHYQSIPRASTPTEALQQHFAFTGADLHPAADPVWPLVNQILGRMMDFLRTELVS
                     SRPVQSPERST"
     gene            2664..3965
                     /locus_tag="PKO06_03470"
     CDS             2664..3965
                     /locus_tag="PKO06_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68731.1"
                     /translation="MPHRHVEIWLGIVGLFAALVVLACGRNVQQEAERGRGHLGRLLR
                     AALALIGLLGLSGSAEAATAAKPANLIETYRETLGRSVSGKHLLETWQAAREIADGTR
                     GAYPFDAAGKKAFLAELARALADVDALVKDRVFQLGEASLLKLELRELTAGVQRRRTT
                     ESQNVTCYRVATVSPVRVSRRRLEARIPLVEKLLPGRVSPLVQETVIKGCAADLAYLQ
                     QQADEKRLPADERAPVEDLIRRARPALERLQVSVQAQDVKGYSRDVWEKIFAAWEQLQ
                     KMVDGGSTTKEREDMGQTLGEIDELLKQQREERRLAYEESESVSQELRFLFEKAREMP
                     PKDSTITCYKMAYVPPAKMSLERLEKRLPLLKRVKAKGQLPKTTLLKMMKAIEVDLTT
                     TEGGVDDPDLSADEREQARRISEQIRLECERLYDVEDRGKK"
     gene            3968..5005
                     /locus_tag="PKO06_03475"
     CDS             3968..5005
                     /locus_tag="PKO06_03475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68732.1"
                     /translation="MSASRPVQQRSFVFEVTGRCNHACPHCYNVWKAVDYPCEELNTA
                     DTLRILDAMLRQSGASLVTLTGGEPLLRPDLYEIVDFLAERGIGINLISNGSLLTESA
                     IARLGPKRISFFELPLLGCCADTHDELSGRRGAFDDVTRAVADLKLAEARVVSVFVAT
                     RRNLSEFRETAELAIALGADGLMLNRFNPGGEGIRHIDELQATPAEMQRALDAAEEIS
                     REYEFPISCSIAMPPCLFDTQKYERLTFGFCAAGTDRAYYTLDPQGNIRPCNHSPTIL
                     GNLRRQSLEEIVNGSAMTAFMAACPEFCAGCRLERECLGGCKAAAEVCYGSPAKADPF
                     LSAFMEQARKL"
     gene            complement(5026..>5767)
                     /locus_tag="PKO06_03480"
     CDS             complement(5026..>5767)
                     /locus_tag="PKO06_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNV68733.1"
                     /translation="TLGDEVCFCSLLGRDHAGRMALETIERLGIDGSRLLATLPETPH
                     SVILFDPTGRRQIHVDLKAIQETPYSVEIAENALDGCSLAVLCNINFSRPLLEVARKR
                     GIVIATDLHALSSIHDSYNQDFLGQADIVFVSHEHLGDAPEMFARHLFDAWHQLQIVV
                     VGMGAEGALLAVRENGVVKRFPAVTVRPIVNTIGAGDALFSGFTHFYAQQRDPERALH
                     LATRFAAWKIGASGGAEGFLTEEELLRL"
BASE COUNT         1194 a         1635 c         1867 g         1071 t
ORIGIN      
        1 agcaccatgc tcaagctctt gccgacttcg tgcagagtgc ccagctcgat gatcttgcgg
       61 tcgagatcga agtagagttt ggcgttcttc atcgcgatgc cggcctgtgt tgccagggtc
      121 tcgagcagtg ccaggtcgtt ggccgtgaag cgctcgccgg tcatcttgtt gccggccgag
      181 atgatgccga tgacatcgtc gttcaccttg atcggcacgc agatcgaact gcgtgaaata
      241 cgcgccggat caagcatcag cgtcttctcg ccctcagcga gatcgttgat gagtatgggt
      301 tctccggtct ggaacacctt gccggcgact ccgtcggtga tcggcacccg gcgattgtcc
      361 ggcaggttcc cgacgtcgag gcccttggcg atcttgatca cgaattcatt gctcggccga
      421 tccgccagca tcagcgaaca gcgtttgact cctcccagaa cctgaatcgt ggtatcgacg
      481 atcacttcat acaggcgatc gagattcaac acggagttga tgatgcgccc ggcttgctgc
      541 agcgtcgaca gctcgaagac acggcgctcg agcgagacgt ttttctgatc gaggtctttc
      601 aggtgagctc cgatgctgtc ggccatgcgg ttgaacgagt cgacgaggga gccaagatct
      661 tcctgcggtt gcccgggaac ccgcgcgtcg agtcggcccg aagacagctg attggcggtt
      721 tcctcgagag agcgcagcgg agctttgagt gcgcgagcca cgactccggc gagcatccag
      781 gcgaagaaca gcagaaagac gaacagccag aggtgcgccc gcagaatctc gtcctgcttc
      841 tgtcccagag gcatggtcga gtattcgagt cgcagatagc ccaagggttc ggtgcgctcc
      901 gggggccgaa tcgggcgcag aaactgaatg atgtcggagg cgcggcggct ctcgaggtcg
      961 accggagccg aggctgggtg cgaatgggcc agcgtgatca tgtcgcgcga gagaaaggcc
     1021 atgccctgca gcgggttgtc tgcggtttca cgatcggtca gtttcagggc gatcgtggtc
     1081 agcaggggaa cgttgcgttc gatcagcagc ggccgcacca gttcgtccag agacttccag
     1141 atgccgttga cgtgcgatct gatgtcgcgc tcatagcttt cacgagcacg ttctgtctcg
     1201 acccagctcg taccacagag ggtggcgagc aacaacagcg acatggccag aaagacccgc
     1261 gcctgcagcg aaatgcggga ggcaggctcg ggtaaagggg tcgtggggat agtggtgtcg
     1321 gtcatgtgga cctctcaggc gattgaacgg gccgcgaact gacaagctcg gtgcgcagaa
     1381 aatccatcat gcgtccgaga atctggttga ccagcggcca gacgggatcg gcagccgggt
     1441 gcaggtcggc gccggtaaac gcgaaatgct gctgtaaggc ttcggtcggg gtgctggccc
     1501 ggggaatgct ctgatagtga atgccttcgg gaaaacgagc ggtggccgcc ccaaaatcgc
     1561 tgctgatgag aatctggtcg ccacgccacg aaaccagcgt acgatgggca ttttccctgc
     1621 cttcgctgac ctgcagcaac agcaggactc gtcgttccgg gccgatccag gcctggccga
     1681 cggtttgaaa gatgggcatt tccagaaggg gcatcgagaa cacggaaagg ggcgtaaatc
     1741 cggcattctg ccagagcggc aactggtctt tcatgtcggt ttccagcagt gggttggggg
     1801 tgccgtcggg aaagaccttg atctcgcgcg gcaggcgcgg gccggggttg cgatcgagat
     1861 gcagaatcga ataacgcgag ggcagtgggt gcagaattcc gcacagaaag aagtagatca
     1921 ggccgtgaag cgccattccc atcggagaga acggcttgcc gggatcgcgg gcatagagcc
     1981 accagaacca caaggccccc agcgccagga cgaacaccca gcgcttgccg aaagcgggaa
     2041 cgcgccgtga ggtgagggcg ggggcgagat agcgaatctg gacgaacagg caccgcagtg
     2101 acagcgtcag aaacagcaga ccgaagaccg ggcgaaacca ctgatagatg ctcggatcac
     2161 tgctgaagcc gaccagcaga atgcccgcga gaaagcccgc cgacagcagc gagtggagac
     2221 ggtgccagac atgaaacttg gtgagatggt tgcgaccggc ttccgtaatg aagacatcgt
     2281 tccagccctt ttcggtgtcg acgcgcaccc gattgagaaa atacgagtcc agggcgtcac
     2341 ccatcatggc gggagcaacc gccatggaag cgaagatcgt ttcccagaaa ccgtcacgcc
     2401 agccggtttt catcagaacg acccaggccg ccagtacgcc cgcaatcgaa aagtagttgc
     2461 tgccgatgcc gagcgtgata ttggtctgga gcttctccat gtcgtttccg gcaccgggta
     2521 gtggaattga catggaatga tcatagcatt tccggacgtg aatttcacga tgaattccgg
     2581 ctgttttcgt gtcggtatgg cacggtgcat ggttcggtgg tcgtggtatc atggcgcgaa
     2641 ttgtttgagt gaggagaaga atcatgccgc accgtcatgt cgaaatctgg cttggtattg
     2701 tgggtctgtt tgccgcgctg gtcgttctgg cctgcggccg caacgtgcag caggaagcgg
     2761 aacggggccg cgggcatctg ggccgcctgt tgcgggcggc tctcgccctg atcggtctgc
     2821 tgggcttgtc gggcagcgcg gaagccgcga cagcggcgaa accggcgaat ttgatcgaaa
     2881 cctatcgtga gacgcttggc cgaagtgttt ccgggaagca tctgctcgag acctggcagg
     2941 cggctcgcga gattgcggat ggcacgcgtg gagcgtatcc gtttgatgcc gccggcaaga
     3001 aggcatttct cgccgagctc gcgcgcgcgc tggccgatgt cgatgccctg gtgaaagatc
     3061 gggtgtttca actcggcgaa gcatcgctgt tgaaactgga actacgtgaa ctgacggctg
     3121 gcgtgcagcg tcgtcgcacg acggagagcc agaatgtcac ctgctatcgc gtcgcgacgg
     3181 tgtcaccggt tcgggtctcg cgtcgacgcc tcgaagcgcg cattcctctg gtcgaaaaac
     3241 ttttgcccgg gcgggtttcg cccctggtgc aggaaaccgt gatcaagggg tgtgcagccg
     3301 atctcgcgta tctgcagcag caggccgacg aaaagcgttt gcccgcggat gagcgtgctc
     3361 cggttgaaga cctgattcgg cgcgcccggc ccgcccttga acgtctgcag gtgtcggtgc
     3421 aggcgcagga cgtgaagggc tactcgcgtg acgtctggga aaaaatcttt gccgcctggg
     3481 aacagctgca gaagatggtg gatggtggca gcacgacaaa agaacgcgaa gacatgggtc
     3541 agactctggg cgagatcgac gaactgctga aacagcagcg tgaagaacgc cgccttgcct
     3601 atgaagagtc cgagtccgtc agtcaggaac tccgctttct gtttgaaaaa gcgcgggaga
     3661 tgccaccgaa agactcgacg atcacctgct acaaaatggc gtatgtgccg ccggcaaaga
     3721 tgagtctcga acggttggaa aagcgcttgc ctctgttgaa gcgggtcaag gcgaagggac
     3781 aactgccgaa aacgaccctg ttgaaaatga tgaaggcgat cgaagtcgat ctgaccacca
     3841 ccgagggcgg ggtcgatgat cctgacctgt ccgcggacga acgtgaacag gcgcggcgca
     3901 tcagcgaaca gatccgcctc gagtgcgaac ggttatacga cgtcgaagat cgaggcaaga
     3961 agtgaatgtg agtgcgagcc gaccggtaca gcagcgctcg ttcgtcttcg aagtcaccgg
     4021 tcgctgcaat catgcctgcc cgcattgtta caacgtctgg aaagcggtcg actacccatg
     4081 cgaggaactg aacacggccg acacgcttcg gatactcgat gccatgctgc ggcagagcgg
     4141 tgcgtcgctg gtgaccctga cgggtggcga accgctgttg cgacccgatc tgtacgagat
     4201 cgtcgacttt ctcgccgaac gtgggatcgg catcaatctg atctcgaacg ggtcgctgct
     4261 gaccgagtcg gcaatcgcgc gtctggggcc gaaacgcatc agtttcttcg aactgccgct
     4321 gctcggctgc tgtgccgaca cccacgatga actcagcggg cgccgcggtg ctttcgatga
     4381 cgtgacgcgg gcggtggccg atttgaagct cgccgaggca cgcgtggtct cggtctttgt
     4441 ggcgacccgc cgaaatctgt ccgagttccg cgaaaccgcc gaactcgcca ttgctctcgg
     4501 agcggacgga ctgatgctga atcgctttaa ccctggcggc gagggaatcc gtcacatcga
     4561 tgaactgcag gcgactcccg ccgagatgca gcgggcgctc gacgcggccg aagaaatcag
     4621 cagggaatac gagtttccga tcagctgctc gattgccatg cccccctgcc tgttcgatac
     4681 gcaaaagtac gagcgtctga cattcggatt ctgcgccgca ggcacggatc gggcttatta
     4741 cactcttgac ccacagggga acatccgccc ctgcaatcac tcgccgacga ttctcggaaa
     4801 cctgcgtcgt cagagtctcg aggaaatcgt gaacggttcg gcgatgacgg cattcatggc
     4861 ggcgtgtccg gaattctgcg ccgggtgccg tctcgagcgc gaatgcctcg gcggctgcaa
     4921 agcggcggcc gaagtctgtt atggctcacc cgccaaagcc gacccgtttc tgtcggcgtt
     4981 catggagcag gcgcgcaagc tctgaccgac agcgacccgt acagctcaga ggcgcagcag
     5041 ttcttcctcg gtgagaaaac cttcggcacc gccgctggca ccgatcttcc aggcggcaaa
     5101 acgggtcgcc aggtgcaggg cgcgctcagg gtcgcgttgc tgggcgtaga aatgcgtgaa
     5161 tcccgaaaaa agggcgtcac ccgcgccgat ggtgttgacg atcggtcgca ccgtcaccgc
     5221 cggaaaacgt ttcaccaccc cgttctcacg caccgccagc agagcgcctt cagctcccat
     5281 gccgacgacg acgatctgca gctggtgcca ggcgtcgaac agatgccggg cgaacatctc
     5341 cggtgcgtcg ccgagatgct cgtggctgac aaaaacgata tcggcctgtc cgaggaagtc
     5401 ctgattgtac gagtcgtgaa tcgacgagag cgcgtgcaga tcggtggcaa tgacaatacc
     5461 tcgttttcgc gccacctcga gcaggggccg cgaaaagttg atgttgcaga gaacggccag
     5521 agagcaaccg tcgagcgcat tctcggcgat ctcaactgag tagggggtct cctgaatcgc
     5581 tttcagatcg acatgaatct gccgtcgccc cgtggggtcg aacagaatca ccgagtgcgg
     5641 ggtttcgggc aacgtcgcga gcagtcgcga gccgtcgatg ccgagccgct caatggtctc
     5701 gagcgccatt ctcccggcgt ggtcccgccc gagcagcgag cagaagcaca cctcgtcgcc
     5761 cagcgtg
//
LOCUS       DAORHD010000107         5751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14231_length_5751_cov_3.543188, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000107 DAORHD010000000
VERSION     DAORHD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14231_length_5751_cov_3.543188"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..1008)
                     /locus_tag="PKO06_03485"
     CDS             complement(388..1008)
                     /locus_tag="PKO06_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68734.1"
                     /translation="MPKEKTPATKPRIPKPPKTPSVDALITAVPFLNLPASAPPDELW
                     KAASVFLSHFDNEAVKRFGKIQAVYYAGQLREARDFTKQAIASFVWTPGPFRFPSELA
                     FKGWQDDVGSLRFNVRLDFSSQTVERYPEFPAPGRWGELMTMLRALLMFLVLDAAQDE
                     VLSLYLGYCRKCGKVFEKTRGDQEYCSKACKSATTQKRYDDKQRRS"
     gene            complement(1265..1338)
                     /locus_tag="PKO06_03490"
     tRNA            complement(1265..1338)
                     /locus_tag="PKO06_03490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1302..1304),aa:Arg,seq:cct)
     gene            1480..2073
                     /locus_tag="PKO06_03495"
     CDS             1480..2073
                     /locus_tag="PKO06_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68735.1"
                     /translation="MHRRMTDQSFLTTCFCLTLALMLGSLPLHAVDPSFEMNRLCRSN
                     IKMLNDATAKFFSENQQDLPLWADFNQVYTMILTTKYVPTKPVPPLPDCGYYLMYRNP
                     KDFSWYCNLHGMNAGDESISFRYHEYRFTAYVQTKFLTHPKYKSHYDELNRWTSYSPT
                     LMENMKFQYNRNPTSTIVMGIIGLGVLWFLYRNLFGA"
     gene            2153..5374
                     /locus_tag="PKO06_03500"
     CDS             2153..5374
                     /locus_tag="PKO06_03500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV68736.1"
                     /translation="MTEPSAPSSTPAIVYIPEASGPLPAVQSPLIGWMVLYVFFFALP
                     IAALYFGYRDYISRQHLEEIGKTSNFFTCELSSLRTRASNTKVLTHLCNMLGGDIRPV
                     IDNPKKLRNVIADFQEQIPATATILVWDTRGNLLFPTATEAGVLADLPRFFQIGNTSL
                     AGEISLQPAASTERFEKEYADEISRLSTVLGSGFPFAKSVTLPGKIIISSDVLHPCIA
                     YWQFFPDTTSGKMGYAVILAKQQLPETFGLSWQLRNNRNANPELTSGFIDLEQNIIDV
                     SYRELEKHAAAFLNEYRQTLVSPVIRGDWVLTVMPLGETSPVRLFNLFSLRRLRQQFE
                     DRDFEAQVIIGTLLLLGALYFGHSYRQRTREGLSIRFRLVGITLLSMFLPIAFLGAVA
                     LHFTLDRSKILAGNTQEMLLQEVKHMDEGIVEFFRAKMAWLRSLKNTLPELATVDVPA
                     LTKRFLELQKAGQLERVYLVAPDGSLLYDQDHLFQEFGRKAFISELGRKTIEYASLKS
                     LTGLSDANARDSIGKDFINNLASGRGVLHKILWPGSAEQVYLFVDVFTPSPQAALKGT
                     GPLAVIITINKTGIEEEYVRKAIATPKRRGSEVSFFAVRRDGSFNTIPELRGAFKANL
                     LPLLDAAQALETPQPERVTVDDRHLLLTIRAGMQLDEYYLGAYTDWDVVMGSIEWFER
                     LSLSTILLSISASLSLVFFLVRSFLTPIRVLSEGTRAISGGDLTRHLPIYDRDELGHL
                     SQAFNEMTRRLNNRLNELTLLYNLAQKASTTHSQREIFELVAQRMREYLAADSCGTAW
                     INEGEGKMNLYLGESPDAEIAERARQATITAITNRALHVETIGEGAILGIPLLFEEKE
                     FGGIYLVYTRTIPNITPEIRSFIEMVQRQLSLIIENQRLFEQAVTDGLTKLYVRRFFL
                     ANLEKELARARRYHLDMSLVMIDIDHFKKFNDNYGHQTGDMVLRETAQRILETIRSTD
                     TPGRYGGEEMAVILPQTGLKDAAMVAERMRVAVEEANYIDKDRTLKVTISVGVTSLLG
                     RQLTMEEMIEECDQCLYKAKDLGRNRVVIAEAKPASLEVIPCD"
     gene            5367..>5751
                     /locus_tag="PKO06_03505"
     CDS             5367..>5751
                     /locus_tag="PKO06_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68737.1"
                     /translation="MIDTVIVFGTTSRQSTDCPSSSGASRNGFTLVEIIMVLFLIGII
                     ALPFTRMFSFGAQGTVESFEHVFAFNLAREKVEEVRSLPFEMVKSDFENFRDIYSDQT
                     DLDQMYTTADVFEKNFTDIVTESRLK"
BASE COUNT         1308 a         1657 c         1477 g         1309 t
ORIGIN      
        1 tttccggact tccggggtag gaccctggca cacacgccga gactcaacga atcgctgaag
       61 gtcaatttct gaaataacca aggagcgccc gccaggcctt actgcaggga taagcccctt
      121 gccaacaagc gttcgaacat aggaaacgga catccggaga attccggcca cctctttggg
      181 ggtaagaaag atgggcataa aaaagccctc ctgagatagc ttctgccaag taaaaccctg
      241 gcagttgtct catggagggc cttttgctat cctgggtttg tcctagaccg gtttacgccg
      301 ggccacccat gagggtgacg gttagaaagt tgctgcttcc tttccgtcgc ccccatcata
      361 gctcttagtc ttttcgatgt caatagacta agatcgtctt tgtttgtcgt cgtatcgttt
      421 ttgcgttgtg gccgacttac atgctttgct gcagtattcc tgatcaccac gggtcttttc
      481 aaagactttg ccacatttgc gacaatagcc aagatacaag cttagaacct catcttgcgc
      541 cgcatcgaga accaagaaca taagcaaagc cctgagcatt gtcatcagtt ctccccatcg
      601 acccggcgca gggaattccg ggtatcgttc aacagtctgg ctcgaaaagt ccagtctcac
      661 attgaatcga agactcccaa catcatcctg ccatcccttg aacgccagct cagaaggaaa
      721 tctgaacggc ccgggggtcc agacaaatga tgcgattgcc tgcttggtaa agtctctggc
      781 ttcacggagt tgcccagcat agtaaaccgc ttggatcttg ccaaaccgct ttacggcctc
      841 attatcaaag tgggatagaa agacagaagc cgctttccag agctcatcag gcggagcaga
      901 agcaggcagg ttaagaaagg gaacagccgt gatcagtgcg tcaacgctgg gcgttttcgg
      961 tggtttcgga atgcgaggtt tggttgcggg tgtcttttct ttcggcatct ggtcaggctt
     1021 tcggggaata ggtgctacgg catatcatgc gagcgacggg catctgagcg ggccagagca
     1081 ttgtatcatg cacgcctgtt tcggggccgg aacgttcagc aaacaagagc gctttcaatc
     1141 agcgttttag aggctccaca cctctgacaa cagcttacag tcagactttc aaacttttgc
     1201 atcccctttt gcatccccgg attaaaacag cccagcaaac cagcatcaga agccgacaag
     1261 acaatgcgcc tacctggact cgaaccaggg gccccaggat taggaatcct gtgctctatc
     1321 cacctgagct ataggcgcag cgggtgaaat gatactcgct ctgcttctga cggtcaactt
     1381 tctacggtcg ccgctccgtc gtctcgcgag gaagatagat gaaaatttgt gggaactggg
     1441 gtaacatgtg accactctcg acgacgcagg agacacgcaa tgcatcgacg catgactgat
     1501 caaagcttcc tgacaacatg cttctgtctg actctcgctc tgatgctggg ctcactcccc
     1561 ctgcacgctg tcgacccgag cttcgaaatg aaccggttgt gccgttcgaa tatcaagatg
     1621 ctgaacgacg cgaccgcgaa gtttttttcg gaaaaccaac aggatcttcc tctctgggca
     1681 gattttaatc aggtctacac catgatcctg acaacgaagt atgtcccgac aaaaccagtt
     1741 ccaccgctgc ctgactgcgg ttattatctg atgtatcgga atcccaagga tttttcatgg
     1801 tactgtaatc tgcatggcat gaacgccggc gacgaatcca tctcgttccg gtaccatgag
     1861 tatcggttta ccgcctacgt gcagaccaag ttcctgaccc atccgaagta caaatctcac
     1921 tatgacgagc tcaatcgctg gacctcctat tctccaacgc tgatggagaa catgaagttc
     1981 cagtacaatc ggaatccgac ctccaccatt gtgatgggca tcatcgggct tggcgtgctg
     2041 tggttcctgt atcgtaacct tttcggcgcc tgatctcgtc tccgtagaaa gttgcgtctc
     2101 ccatccatct cagatatcct gaaacacaat ccatgagaga acccaacgat cgatgacaga
     2161 accgagcgcc ccttccagca caccggctat tgtctacatc cccgaagcat cggggccgct
     2221 gccggctgtt caaagtccgc tgatcggatg gatggtcctg tatgtattct ttttcgctct
     2281 ccccatcgcg gccctgtatt tcggatatcg ggactacatc tctcgccagc atctcgaaga
     2341 gatcggcaag acatcgaact tcttcacgtg cgaactgtcc tcgttgcgca cccgggcatc
     2401 caataccaaa gtcctgaccc acctgtgcaa tatgctcggc ggcgatattc gccccgtgat
     2461 cgacaatcct aaaaaattac ggaacgtcat tgcggacttt caggaacaga tcccagccac
     2521 agcgacgatc ctggtctggg acactcgcgg caatctcctg ttcccgacgg ccacggaagc
     2581 cggcgttcta gccgatctcc cgcgattctt tcaaattggc aacacctcgc tcgccggaga
     2641 aatttcgctc caaccggccg cctccaccga gcgttttgaa aaagagtatg ccgatgaaat
     2701 cagccgcctc agcaccgttc ttggcagtgg gtttcccttc gccaagagcg tcacccttcc
     2761 gggcaaaatc atcatcagta gcgatgtctt acatccctgc atcgcctatt ggcagttttt
     2821 tcccgatacg acttcgggaa aaatgggcta cgccgtcatt ctggccaagc agcagttgcc
     2881 ggaaacattc ggtctgagct ggcagctgcg caacaaccgc aatgccaatc cggaactgac
     2941 cagcggcttc atcgacctcg aacagaacat aatcgacgtc tcgtatcgcg agctcgagaa
     3001 gcatgccgcc gcttttctga acgagtaccg acagaccctg gtttccccgg tgattcgcgg
     3061 ggactgggtg ctcacggtca tgccccttgg cgaaacctcg cccgtccgtc ttttcaacct
     3121 gttttcactg cgccgtctgc ggcagcagtt tgaggaccgc gacttcgagg cgcaggtgat
     3181 catcggtacg ctgctgctgc tgggggcgct ctattttggc cactcctacc gccagcgaac
     3241 ccgcgagggt ttgtcgattc ggtttcgtct cgtaggaatc acgcttctgt ccatgtttct
     3301 gccgatcgcc tttctggggg cggtggcact gcatttcacc ctggaccgct cgaagattct
     3361 cgccggtaac acgcaagaga tgctgttgca ggaagtcaag cacatggacg aagggatcgt
     3421 cgaattcttc cgcgccaaaa tggcctggct gcgttcgctc aagaatacgc tccccgagct
     3481 cgccaccgtt gacgtcccgg ctctgacgaa acgctttctg gaactgcaga aagccgggca
     3541 actcgaacgc gtgtatctgg ttgctccgga cggaagtctg ctgtatgacc aggatcacct
     3601 gtttcaagag ttcggacgta aggcgtttat cagcgagctc ggccgcaaga caatcgagta
     3661 tgccagcctg aaaagcctga ctgggctgtc agatgcaaat gcgcgagatt cgatcgggaa
     3721 ggatttcatc aacaatctcg cctctgggcg tggagttctg cacaagattc tctggcccgg
     3781 gtccgccgaa caggtgtatc tgttcgtcga cgtcttcacc ccttccccac aagcggcatt
     3841 gaagggcacc ggaccactcg ccgtcatcat cacgatcaat aaaaccggta tcgaagagga
     3901 atacgttcgc aaggcgatcg ccactcccaa acgtcggggt tccgaagtca gcttcttcgc
     3961 ggtgcgacga gacggttcct tcaatacgat tcctgagctc aggggcgcat tcaaggcaaa
     4021 cctgctcccc ctgctggatg cggcacaggc tttggaaacc ccgcaaccag agcgagtgac
     4081 cgtcgatgat cggcatctgc tcctgaccat tcgtgcaggc atgcaacttg atgaatacta
     4141 cctgggggca tacaccgact gggacgtcgt catgggttcg atcgaatggt tcgaacgttt
     4201 gtctctgagc accattctgc tcagcatctc ggcatccctg tccctggttt tctttctggt
     4261 tcgcagcttt ctgactccga ttcgcgtact ttccgaagga acgcgagcca tttcgggcgg
     4321 tgatctgacc cgacatctgc cgatttatga ccgtgacgaa ctcggacatt tgtctcaggc
     4381 attcaacgag atgacgcgcc gcctgaacaa ccgactgaat gagctgacgc ttttgtacaa
     4441 cctggctcaa aaggcttcga cgacccatag ccagcgagag atctttgagc tcgtggccca
     4501 gcgcatgcgc gagtatctgg cggccgactc gtgcggtacc gcctggatca acgagggcga
     4561 gggcaagatg aacctgtatc tcggcgagtc tcccgacgcc gagatagccg agcgcgcccg
     4621 gcaggcaacc atcacggcga tcacgaatcg cgcccttcac gtcgaaacga tcggcgaagg
     4681 cgccattctc ggcattcccc tgctgttcga ggaaaaagag ttcggcggca tctacctggt
     4741 gtatacgcgc acgattccga acatcacacc ggagattcgc agtttcatcg agatggtgca
     4801 acgccaactg tcactgatca tcgagaatca gcgactgttc gaacaggcgg ttaccgacgg
     4861 tctgaccaag ctgtatgtcc gacggttctt tctggccaat ctcgagaagg aactggctcg
     4921 agcgcgccgc tatcatctcg atatgtcgct ggtcatgatc gacatcgacc atttcaagaa
     4981 gttcaacgat aattatggcc atcagacggg cgacatggtg ctgcgagaga cggcacagcg
     5041 aatcctcgaa accatccgct ccaccgatac tcccggtcgc tacggcggcg aggaaatggc
     5101 cgtcattctt ccgcagaccg ggctgaagga tgccgccatg gtggcggaac gcatgcgcgt
     5161 ggcggttgag gaagccaact atatcgataa ggatcgaacc ctgaaagtca ccatttcggt
     5221 cggagtcacg tcgcttctgg ggcgccaact gacgatggaa gaaatgatcg aagagtgtga
     5281 tcagtgtctg tacaaagcca aagatctcgg ccgcaaccgc gtcgtcatcg cggaagcaaa
     5341 accggcaagc ctggaagtca ttccctgtga ttgacacggt catcgttttc ggtaccacct
     5401 cgcgacagag tactgactgc ccatcgtcgt caggcgcttc cagaaacggt ttcaccctgg
     5461 tcgaaatcat catggttctg tttctcatcg gcatcatcgc tctgcctttc acgcgtatgt
     5521 tctcattcgg agctcaggga accgtcgaaa gtttcgaaca cgtcttcgcc ttcaatctcg
     5581 cacgtgaaaa ggtcgaggaa gtgcgttcgc tcccgttcga aatggtgaaa tcggacttcg
     5641 agaattttcg cgatatctac agtgaccaga ccgatctcga ccagatgtac accactgccg
     5701 acgtgttcga gaagaatttt accgatatcg tcaccgagag tcgcctgaaa g
//
LOCUS       DAORHD010000108         5730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14295_length_5730_cov_4.954890, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000108 DAORHD010000000
VERSION     DAORHD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14295_length_5730_cov_4.954890"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKO06_03510"
     CDS             complement(<1..874)
                     /locus_tag="PKO06_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68738.1"
                     /translation="MKLRILVVSFCCCLAGLWAAPSALVNQPADLRLIDLRENARFLE
                     EDLDLGKGITEVIGKQVIDRRFRSAGIPDPVKKQLSYGIVAREMPVDSLNVCFVLKGD
                     LDTDKFLAFADKRYQRYFDTIRAQKLIEQTPTPRFIRVAGKSARIYPFAFRQAEAVVT
                     TFPGHVIISTVPAGDYSLIEETVGVLEGRIPASTEQPEKIGFMTTFVPLAQERNEIRQ
                     FENRYEGFAAKTRKQFRKIAAPEKFRNDEAMAKAEQQLKAALAEISKFSYEIGARRET
                     EGYAYEVNMIFRCGS"
     gene            1025..2422
                     /locus_tag="PKO06_03515"
     CDS             1025..2422
                     /locus_tag="PKO06_03515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68739.1"
                     /translation="MKCLLVLPHGPIHRAGTGSYRKSLRYAPLTLATLASLVPAELGL
                     ELRIIDEGVDSWDPRQHLDVDLIGISTMTGTANRAYAIGDFFRRHGKRVIMGGIHATL
                     LPHEAKSHADCVITGLAEDTWPQALRDAVANRLQPFYHMRPDFDWTSIKRPMPDRRIF
                     DRRKYVTVNSIEATRGCAHLCSFCAVATAWDHRYYMRPVEEVIAEIQTLQGDELCFLD
                     PSLTCNREYSLRLFEALRPLGKWWVGCATIDVAHDPTFLSALAASGCRGLLIGFESVS
                     QASLHEVRKPFNDVTKYKSAVRKFHDHGIAIQACFVFGLDTDDQDVFKRTVQFVYDCE
                     IDLPQFSVLTPFPGTAVFTDLERQGRLIERNWSLYDAEHVVFRPLGMSPERLQDGLFY
                     AWKHAYSLRSIFRRLSGSRCLLSIAIPANLGYNVYARHLSRYTPDRMENEGVLFPEPG
                     ASAPPATESLAGQQA"
     gene            complement(2454..4082)
                     /locus_tag="PKO06_03520"
     CDS             complement(2454..4082)
                     /locus_tag="PKO06_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNV68740.1"
                     /translation="MLNRALCAACHAAWLTTCLPQTLAFARASRSSVRHIQEQVLVEI
                     LQRQATTVFGRRHRFDRLHSACEFQATVPPAEYEDLAPYIEAAAAGAPHQLTVEPVHV
                     FEPTSGSTAAFRLIPYTSASQDSFNRALHPWLFDLFTHRPHLATGPAYWVITPRLGVA
                     ERTSSGVPIGFVDDAAYFGRFAKALIDRVMVVPSSVGRLKDPREWRLQTLWRLVRAEQ
                     LRLISLWSPTFLPVLLRSLPEFAADLQQMLAGEDRDRLDLVNRVIEEIRAGREMGCGT
                     KLWPQLQLISTWTDGDAAAGLPDLQKWFFGVPLQPKGLLATEGVVTIPMCEASAPVLA
                     VRSAFFEFIEGEEGEGSYRLAHELEQGGRYRVLMTTPGGLYRYKLHDVVSVEGWWRRL
                     PMLQFIGKEAHVSDLCGEKLNARHLREILQTLVGSLNGVFVAAETASSPPHYVCLIST
                     DVEAPGASRLRNELEAQLQKNPHYRWCREVGQLNELRVVQIPYDTVEMNRRRLARWES
                     MGTRRGTAKTGVLDRLTGWTGYLTGDTVDSSLAR"
     gene            complement(4155..4874)
                     /locus_tag="PKO06_03525"
     CDS             complement(4155..4874)
                     /locus_tag="PKO06_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68741.1"
                     /translation="MGRSADRELRGSIVRVAEIATSVRDAMFGLMDEAYVVGRESFLA
                     DLAAKDWAILLHDQQGGLRGFTSLAVMDADVGDRSIRALFSGDTIIQPEFWGSLELPR
                     VWGRFMLQAIEEAGARELYWFLISSGYKTYRFLPVFFREFFPRFDCPTPNDLQAILDS
                     IGEKRFHARFDRERGIIRLEHPTPLRPGIADLTPERLQDPHVAFFVDRNPGYDRGEEL
                     ACIARLTVDNLRPFIRRALKA"
     gene            complement(4876..>5730)
                     /locus_tag="PKO06_03530"
     CDS             complement(4876..>5730)
                     /locus_tag="PKO06_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68742.1"
                     /translation="QAGGTLLARGYQAFREVSREFPAPITTTSILEGNTTARGLLENR
                     AGRGPLPAYEPLTRYLTALLPLRGPGARWPRRMRTRNVSPYVIRNLASEDQTAVSDLY
                     ERASRSFDGLVLPIIVNHHSDAGLWPGLNWSDMLGVFAGRDLLGLIAVWNRMPVQQIR
                     LSHLHPVLTFLQRFWSVGRAFWGECPLPPSGTEVPHLLLDPWLIAPGREREIGPLLLD
                     AALTRAAARHALFVAFGVPEWHPMREVIRRFFHVPYWSMLYTVRWPETPAVPTGVEKR
                     LIPPLGTL"
BASE COUNT         1255 a         1765 c         1593 g         1117 t
ORIGIN      
        1 ttgagccgca gcgaaagatc atgttgactt cgtaggcata cccctcggtt tcgcgtcggg
       61 caccaatctc gtagctgaac ttgctgatct cggccagagc ggccttcagt tgctgttcgg
      121 ctttggccat ggcttcgtcg ttgcgaaact tttcgggagc ggcaatcttg cgaaactgtt
      181 tacgtgtctt cgcggcaaac ccttcgtacc ggttctcaaa ctggcgaatc tcgttccgtt
      241 cctgggcaag aggcacgaat gtcgtcatga atccgatttt ttcgggctgt tcggtggagg
      301 cgggaatccg gccctcgaga acacccacgg tttcttcgat gagagagtaa tcaccggccg
      361 gcaccgttga gataatgacg tgaccgggga aagtggtcac cacggcttca gcctggcgaa
      421 acgcgaaagg atagatgcga gcggacttgc cggccacgcg aatgaagcgc ggtgtcgggg
      481 tctgttcgat gagcttctgc gctctgatcg tgtcgaagta gcgctggtag cgtttgtcag
      541 cgaatgccag aaacttatcg gtatcgaggt cgcccttcag aacgaaacag acattgagcg
      601 aatcaaccgg catctcacgg gcgacaatgc cgtagctgag ctgctttttg accgggtcag
      661 ggatgcctgc cgaacggaaa cgccgatcga taacctgctt gccgatgact tccgtgatcc
      721 ctttgccaag atcgaggtct tcttcaagaa aacgagcatt ctcgcgaaga tcgatgagcc
      781 gcagatcggc cggttggttc accagagcac tgggagcagc ccacaggcca gccagacaac
      841 aacagaacga caccaccaga attcgcagtt tcatgtgcga tcctcctctg aaaaaactcg
      901 tcgacaacca actctacgtt acaccacttt tcttctccac ttcaacattg aataacctcc
      961 agccgaccac tatcatgagc ttgcggaaat ctcatcagtc tgttcgtgac ggagaaggat
     1021 cagcgtgaag tgcctgcttg tcctgcctca cggcccgatt catcgggccg gaaccggttc
     1081 gtacagaaag tcgctgcgat acgctcctct gactctcgca accctggcgt ctctggttcc
     1141 tgccgaactc ggactggagt tgcgcatcat cgatgagggg gtcgactcct gggatccacg
     1201 ccaacatctc gatgtcgatc tcatcggcat cagcaccatg acgggaacgg cgaatcgagc
     1261 ctacgccatc ggggactttt tccgccgaca tggcaagcgc gtcatcatgg ggggcattca
     1321 cgccacgctc ctgcctcacg aagccaaatc tcacgccgat tgcgtcatca ccggactcgc
     1381 ggaagacacc tggcctcagg ctctgcgaga tgcagtcgcg aaccggttgc aaccgtttta
     1441 tcacatgcga cctgacttcg attggacttc gatcaagcgt cccatgcccg atcgccgcat
     1501 cttcgaccgt cggaagtacg tgaccgtgaa ctcgatcgaa gcgacacgcg gctgcgccca
     1561 tctctgttcc ttctgcgcgg tcgccaccgc ctgggaccac cgatactaca tgcgaccggt
     1621 cgaggaggtg atcgccgaga tccagaccct gcagggcgac gaactttgtt ttctcgaccc
     1681 cagcttgacc tgcaaccgtg agtattcgct gcgtctgttc gaagcgcttc ggccgctcgg
     1741 caagtggtgg gtgggctgcg ccaccatcga tgtagcgcac gacccgacat ttctctcagc
     1801 cctggccgca agcggctgcc gcggattgct gattggcttc gaatccgtgt cgcaggcgtc
     1861 actgcatgaa gtgcgcaagc cattcaacga cgtgaccaag tacaagtcgg ccgtgcgaaa
     1921 gtttcacgat cacggtatcg ctattcaggc ctgctttgtc ttcggtctcg acacggacga
     1981 tcaggatgtt ttcaagcgga cggtccagtt cgtctacgac tgtgagattg atctgccgca
     2041 gttttccgta ctgacaccct ttccaggaac cgccgtgttt accgatctgg aacgtcaggg
     2101 tcgcctcatc gaacgcaact ggtcactgta tgatgccgaa catgtcgtat tcaggccgct
     2161 cggcatgagt cccgaacgtt tgcaggatgg tctgttctac gcctggaaac acgcctattc
     2221 gctgcggtcg atattccgcc gactgtcggg atcgcgttgc cttctctcga ttgccatccc
     2281 cgccaacctg ggctacaacg tgtatgctcg tcatctctcg cgttacactc ccgaccggat
     2341 ggaaaacgag ggagtactgt ttccagaacc gggggcttcg gcaccaccag ccaccgaatc
     2401 actcgcaggc cagcaggcct gaacctctcg atatcggctg ctgatcagcg ggctcagcgt
     2461 gcaagagacg aatcgaccgt gtcgcccgtg agatagcctg tccatcccgt cagccgatcg
     2521 agcacccccg tcttggccgt tccacgccgg gttcccatag actcccagcg ggccaggcgg
     2581 cgacggttca tttcgaccgt atcatacgga atctgtacta cccgcagctc gttcaactga
     2641 ccgacttctc ggcaccagcg gtagtgtgga ttcttctgca gctgtgcttc gagttcgttc
     2701 cgcagacgac ttgcacccgg tgcttcgacg tccgtggata tcaggcacac atagtgtgga
     2761 ggagagcttg ctgtctcagc cgccacaaaa accccgttca gcgaccccac cagggtctgc
     2821 aggatctcgc gcaggtgacg ggcattcagt ttttcaccac agagatccga gacatgtgct
     2881 tccttgccaa tgaactgaag catcggcaat cgacgccacc atccttctac cgagaccacg
     2941 tcatgcagtt tgtaccgata aagtccacct ggagttgtca ttaacacgcg ataacgacca
     3001 ccttgttcca gctcgtgggc gagacgatac gagccctcac cctcttctcc ttcgatgaac
     3061 tcgaagaaag ccgagcgaac ggcgagaacc ggtgctgatg cttcacacat cggaatcgta
     3121 accacccctt cggttgccag caatcccttg ggttgcagtg gcaccccgaa aaaccacttc
     3181 tgcagatcag gcaagccagc tgccgcatca ccgtccgtcc aggtcgatat gagctgcaat
     3241 tgtggccaga gcttcgttcc acaccccatt tctcggccgg cacgtatttc ttcgatgaca
     3301 cggttcacca aatccagccg gtcacgatct tcgccagcca gcatctgctg cagatctgcg
     3361 gcaaactcgg gcagagaacg cagcagaacg ggcagaaacg tcgggctcca gagcgaaatc
     3421 agacgcagct gttccgcacg caccaggcgc cagagggtct gcaaccgcca ctctctcggg
     3481 tccttgagac gtccgaccga ggagggaacc accataaccc gatcaatcaa cgctttcgcg
     3541 aagcggccga agtatgccgc atcatcgaca aaaccaattg ggacaccact cgacgtgcgt
     3601 tccgccactc ccagtcgcgg agtgatcacc cagtaggccg gcccggtcgc cagatgcggt
     3661 cgatgggtga acaaatcaaa caaccaggga tgcaaggccc ggttaaagga atcctgcgaa
     3721 gcgctggtgt agggaatcag gcgaaaagcc gccgtcgaac cactggtcgg ctcaaacacg
     3781 tgaaccggtt cgaccgtcag ctgatggggc gctccggcag cggcggcctc gatgtagggc
     3841 gcaagatctt catattcggc gggcggaacc gtggcctgaa actcgcaggc cgaatgcagg
     3901 cgatcgaatc gatgtcgtcg accgaacacg gtcgtcgcct ggcgctgcag gatctccacg
     3961 agcacctgtt cctgaatgtg tcgcaccgaa ctgcgtgacg cacgggcaaa cgccagcgtc
     4021 tgtggcagac aggttgttaa ccaggcagcg tggcaggccg cacacagcgc tcggttcagc
     4081 acgacctcac gtctctcctt tgccggcaac ccgattcggg tcagtcggcc cacgaatcgc
     4141 agaccctcgc tggctcacgc cttcaaagcc cgccggatga acggtcgaag attgtctacc
     4201 gtgagtcgag caatgcaggc gagttcctcg ccgcgatcat aaccagggtt tcggtcaaca
     4261 aagaacgcga cgtgcggatc ctgcagtcgt tcgggagtga gatccgcaat cccgggtcga
     4321 agcggtgtcg gatgttccag tcgaatgatc ccacgctccc ggtcaaagcg agcgtgaaag
     4381 cgcttttcac caatggaatc gagaatcgcc tgcaggtcat tcggagtcgg acagtcgaat
     4441 cggggaaaaa actcgcggaa gaacacggga agaaaacgat aggttttgta gccgcttgaa
     4501 atgaggaacc agtagagctc acgcgctcct gcttcctcga tggcctgcag catgaatcga
     4561 ccccagaccc gcggcagttc aaggctgccc cagaactccg gttgaatgat cgtatcgcca
     4621 ctgaagaggg cgcgaatgga tcgatccccg acatccgcat ccatgaccgc aagcgaagtg
     4681 aacccgcgca gccccccttg ctggtcgtgc agcagaatcg cccagtcttt ggccgccagg
     4741 tcggccagaa atgactcgcg accaaccacg taggcttcat ccattaaccc gaacatggca
     4801 tcacgcaccg acgtcgcgat ctcggcaacg cgaacaatgc ttccacgcag ttcccggtcg
     4861 gcagatcggc ccatgttaca aggttcccag ggggggaatc aggcgcttct cgacacctgt
     4921 tggcacagcc ggagtctccg gccaacggac agtatacagc atactccagt acggcacgtg
     4981 aaaaaaacgg cgaatcacct ctcgcatcgg gtgccattcc ggcaccccaa aagccacgaa
     5041 cagagcgtgt cgcgccgcgg ctcgcgtcag tgcggcatca agcagcaagg gaccgatctc
     5101 acgttcccgg cctggtgcaa tcagccacgg gtccagcaac agatgcggaa cttccgtccc
     5161 cgaaggcggt aaggggcatt ctccccagaa cgcgcgaccg acggaccaga accgctgcag
     5221 gaacgtcagc accggatgca gatgagagag gcgaatctgc tgcaccggca tgcggttcca
     5281 gaccgcgatc aggccgagca gatcacggcc ggcaaacacg ccgagcatgt cagaccagtt
     5341 caaacccggc cagagaccgg cgtcggaatg gtggttcacg atgatcggca gaaccaggcc
     5401 atcgaacgat ctcgacgcac gttcgtacaa gtcactaaca gctgtttgat cttcagaagc
     5461 aaggtttcgt atcacgtagg gagaaacgtt ccgagttcgc attcttcgcg gccagcgtgc
     5521 accgggacct cgcagtggca gcagcgccgt cagataacgg gtaagcggct cataggccgg
     5581 aagtggcccg cgaccagcac ggttttcaag cagaccccgc gcggtggtgt ttccttcgag
     5641 aatcgaggtc gtggtgatcg gggccggaaa ctctcgggaa acctcacgaa acgcctgata
     5701 cccacgtgcc agcagagtgc cacccgcctg
//
LOCUS       DAORHD010000109         5689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14454_length_5689_cov_4.161697, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000109 DAORHD010000000
VERSION     DAORHD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14454_length_5689_cov_4.161697"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKO06_03535"
     CDS             <1..861
                     /locus_tag="PKO06_03535"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HNV68743.1"
                     /translation="QKRIPLGMLTVTNNSGGGQPVSMENIRETSKLFRKHKIPFFIDA
                     CRFAENAYFIKTREPKYRKTPVIDIAREMFSYADGCTMSAKKDGMVNIGGFLAMNDDT
                     LAMHARNVLILTEGFPSYGGLAGRDLEALAVGLEEVLHEDYLAYRIRTAEYMGERLLK
                     AGVGIIRPTGGHAVYIDATSVYPHIPAHQFPGQVLVCELYKVGGIRGVEIGSVMFGKS
                     DAKGKPIPAAMELVRLAFPRRVYTQSHFDFAIEAIIETYQQRRKAKGLKITWEPPFLR
                     HFTAQFAPVK"
     gene            920..>5689
                     /locus_tag="PKO06_03540"
     CDS             920..>5689
                     /locus_tag="PKO06_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68744.1"
                     /translation="MTLRRLGLLMILCLLWAGAAMPLWAKKAARIATAPAFLTQDQDK
                     LIKNEVITFLRRNSERKPGEGWLFLARQYRELGQLERAADVLRQILRAAPVSEEQKWD
                     AQLLLGQILVERKEYDAALKEFDRLISWMPVRRWRVEAKVERAKLLSRTVTGLPDLFK
                     AFRRYFAPFPEVSDDEEVRYLIGFERGYDLEIAMRALEAWEEIARYPERPVADLACLN
                     LALIYTYDLGKPERAEPFLASMTVPYRSAAASSTLLIRGTVEQFYRQPRNATQAMELY
                     RSYLQGVRDLDGWRVATVLSGRLLAEDLGEPQTAIEVFSSLASPPRHLLATPSLSLQK
                     RQEVAEEDKFWGVLGYKMAGYTAEYLVKDPDRARACYLKAQEIDRTRVGIQADFWVED
                     ALRRTEPKMSQAELHFEQAYEKYRGRDPQEAIRLWESFVASYPEHVLAREACFRIAVA
                     THDDLKQLDRALELYERYLIRVMPQKSVWKLDKIYDWGRIDEVRYRIGSLKANHRKDP
                     LSAIKIFSELSTLYPDSYWAMQGMKDTIRLQRELLGDENSARQTMRDFIDRYPDTVDA
                     QAYRKELAEKLVEQGDVLEAADLLRGFLDHGLPSDEGYLTVEKQWRELSFRAQEKALR
                     ELIRTSASADRQMLYEELIDVMGLASSSAPLETLVEELKDSRVSDESRWLLTYQLGTR
                     LYRDYPEKAKTVFQTLADSASGPAQLACVLTLGNLAYRVEKSAVGALHYYERAASLTP
                     PLHPLLETPRYRLGRLYLQQNRGIDGLQALTEFVRQYPRSRHLPQAFSAMGDAYAALH
                     HPVRAKQFWAKAVRLLPSLKEKIEKKVADAAKLADPDAWLKSRVDERQRQREEEQARQ
                     ASAAAGLATDSVQTDKTTAEDREPTALFLAATGTVKIDPKATAEELYAAYRDEQQRMP
                     KPDPDNSVTLLLAILKKEAPTPVVDKAMRHFISWRVLRQPRPKDFVAESQEILTKRNY
                     PVVLSELLYRLAEAKERQLKDYEGANKTYFEYLSFFPQGGRVLRVRERIPQVYALAKD
                     LKNALRFNEKLINDTQIPADMRVDASLRKARLEETTDEKEEAVKTLEAALSFESKRRP
                     ELYLQLERLTSTFEYVQKALDTPGEETFRFEALQRLIRKAEEDQKHDEAARLVREYQK
                     AFTGEEAVAWIAKKGEDLGKRGTIAEIEAKIEGFPEEPETAARLYKLAQLVEGMENTK
                     YRSQDLFYELTLVYPGSPFYRESKLRAENVRVIKSIDQIGGLLKKGVKTGEGDALLLH
                     RARLYRDTLQDTARAREDYEALLKLFPDSPRLDEAYLGLGSLILKVDRDGEKAREYWE
                     KGLGVSRNPEVRRQLARNIDDLRQFREKILYSEKNTDHAAGTDLVWKRARLENDRDGA
                     QALVQEALRQLENRPQRARLHYLNGRLFEEAERWGEAISEYEAAHACLYHPGCRKDRV
                     LYRLGRLYARTGKMEEAYRRCVALVTRYPRSRFSRSGFYWLYKQDLAAKRFNLAFDRL
                     GRLLQFRNLFPTHRQAITKLQKEVGARMNLADMQRLQALAKTDGGAAFHYYIGRVLEN
                     DMGDVDGAMREYETYLRSNPPIARARDLLMKLAQLAES"
BASE COUNT         1268 a         1501 c         1724 g         1196 t
ORIGIN      
        1 cagaagcgta ttccgctcgg catgctcacg gtcaccaata attcgggtgg cggtcagccg
       61 gtctccatgg aaaacattcg cgagacctcg aagctgtttc gcaagcacaa gattccgttc
      121 ttcatcgacg cctgccggtt cgccgagaat gcctacttca taaaaacgcg tgaacccaag
      181 tatcgcaaga cgcccgtaat cgatattgca cgtgaaatgt tctcgtacgc tgatggttgt
      241 acgatgagcg cgaagaaaga tggcatggtc aacatcggcg gctttctggc catgaacgat
      301 gacacgctgg cgatgcacgc acgcaacgtg ttgattctga ccgaagggtt tccgagttac
      361 ggtggtctgg ccggacgcga tcttgaagct ttggctgtcg gcctcgaaga agtgttgcac
      421 gaggattatc tggcgtatcg cattcgcacg gccgaataca tgggcgagcg gctgctcaag
      481 gccggagtgg gcattattcg tccgaccggc ggccatgcgg tttacatcga cgcgacttcg
      541 gtctacccgc acattccggc gcatcagttt ccggggcagg tgctggtgtg tgagctatat
      601 aaagttggcg ggatacgagg ggtcgagatc ggcagcgtca tgttcgggaa gtctgatgca
      661 aaaggaaagc cgattccagc ggcgatggag ttggtgcgac ttgcctttcc gcggcgcgtc
      721 tatacccaga gtcacttcga tttcgccatc gaagcgatca tcgaaactta ccagcagcgt
      781 cgcaaggcca aaggactgaa aatcacctgg gaaccgccat ttctgcggca tttcacagcg
      841 cagttcgctc cggtgaagtg acccctgagg ggttgcggaa agcgagcgaa cgtggcacat
      901 tgaggcgggt cccgttcaca tgacactcag acgccttggc ttgctgatga tcctgtgtct
      961 gctttgggca ggtgcagcaa tgcctctttg ggcgaaaaag gcggcacgaa tcgctaccgc
     1021 accagccttt ctcacccagg atcaggacaa actgatcaag aacgaagtca tcacttttct
     1081 gcggcgcaac tcagaacgaa agcctgggga aggctggctg tttctggcca ggcagtatcg
     1141 agaacttgga caactggagc gcgctgcgga tgtgttgcgt cagatcctgc gcgcagcacc
     1201 ggtttctgaa gagcagaaat gggacgctca attgttgctt gggcagattc tggtggagcg
     1261 taaggaatat gatgcggctc tgaaagagtt tgaccgtctg atctcctgga tgccggttcg
     1321 tcgctggcga gtggaagcca aagtggagcg cgccaagttg ctgagtcgaa ccgtgaccgg
     1381 cctacccgac ctgttcaagg cctttcgccg ctattttgcg cccttccctg aagtttccga
     1441 tgacgaagaa gttcgctatc tgattggttt tgagcgcggc tacgatctgg agatcgccat
     1501 gagagcgctc gaagcctggg aagaaatcgc ccgctatccg gaacgtccgg tagccgatct
     1561 ggcctgtctc aatctggcgc tgatctatac gtatgatctc ggcaagcctg aacgagccga
     1621 gcctttcctg gcttcgatga ctgtcccgta tcggtcggct gccgccagca gcaccctgct
     1681 cattcgcggc acggtggaac agttctatcg acagccgcgc aatgcgacac aggcgatgga
     1741 gctgtatcgt tcgtatctgc agggagtgcg tgatctcgac ggctggcgag tggccacggt
     1801 tctttctggg cgtctgctgg ccgaagacct gggtgagccg cagaccgcga tcgaggtgtt
     1861 ttcctcactt gcttctccgc cgcgacattt gctggcgact ccttcactct ctctgcagaa
     1921 aagacaggaa gtcgcggaag aggacaagtt ctggggagtg ctggggtaca agatggccgg
     1981 atacaccgcc gagtatctgg tgaaagaccc tgatcgtgcc cgtgcctgct atttgaaggc
     2041 gcaagagatt gatcgaaccc gtgtcggcat tcaggctgac ttctgggtcg aagatgcgtt
     2101 gcgtcgcact gaaccaaaaa tgagccaggc cgaactgcac ttcgaacagg cctacgagaa
     2161 gtatcgcggt cgagacccgc aggaagcgat tcgtctctgg gaatcattcg ttgccagtta
     2221 ccccgagcat gtactggctc gcgaagcctg ttttcgtatt gccgtggcta ctcacgatga
     2281 tctgaagcag cttgatcgag cgcttgagtt gtacgaacga tatctgattc gggtgatgcc
     2341 gcagaaatcg gtgtggaagc tcgacaaaat ctatgactgg gggcgcattg acgaggtgcg
     2401 ctatcgcatc ggcagtctca aggcgaatca tcgcaaagat ccgctcagcg cgatcaagat
     2461 cttttccgaa ctgtcgacgc tgtatcctga cagttactgg gcgatgcagg ggatgaagga
     2521 cacgatcaga ttgcagcgcg aacttctcgg tgacgagaat tcggctcgac agacgatgag
     2581 agacttcatc gaccggtacc cggacacagt cgatgctcaa gcttaccgca aggaactggc
     2641 ggagaaactg gtggaacagg gagatgttct cgaggcggcc gatctgttgc gcgggtttct
     2701 tgaccatggg ttgccttcgg acgaggggta tctgactgtc gagaagcaat ggcgtgagct
     2761 gtcgttccgg gcccaggaaa aggcacttcg cgagctgatc agaacctcgg cttcggccga
     2821 tcggcagatg ctttacgaag aactgatcga tgtcatggga ctggcgtcct cgagtgcgcc
     2881 acttgaaacc cttgttgaag agttgaaaga ctccagggtt tctgacgaat cccgatggct
     2941 cttgacctac cagcttggca cgcgactcta tcgtgattac cccgagaaag cgaagaccgt
     3001 gtttcagact ctggcggatt ccgcttctgg gccggctcaa ttggcctgcg ttctgacgct
     3061 gggcaatctg gcttatcgcg tcgaaaaatc ggcggtgggg gcactgcact actacgagcg
     3121 ggctgcctcg ttgactcctc ctctccatcc gttacttgag acaccgcgct atcgtctggg
     3181 aaggttgtat ttgcagcaaa atcgcggcat cgatggactg caggcgttga cggagttcgt
     3241 tcgacagtat ccgcgcagtc gccacctgcc gcaggcgttc tcggcaatgg gtgatgccta
     3301 tgcagcactg catcatccgg tcagggccaa gcagttctgg gccaaagccg ttcgactgct
     3361 gccgtctctg aaagaaaaaa tcgaaaagaa ggtcgctgat gccgcgaagc tggccgaccc
     3421 cgatgcctgg ttgaaatcac gcgtcgatga acgacagcgt cagcgcgaag aagaacaggc
     3481 gcgccaggca tcggcggcgg ccggtctggc aaccgacagc gtgcagacgg ataaaacgac
     3541 cgccgaagat cgcgaaccga cggctctttt ccttgctgcc actggtacgg tgaaaatcga
     3601 tccgaaggca actgctgaag aattgtatgc tgcttatcga gatgaacagc agagaatgcc
     3661 caaaccagac ccggacaaca gcgtgactct tctgctggcg attctgaaaa aagaagcccc
     3721 gacacccgtg gtcgacaaag ccatgcgtca tttcatttcc tggcgcgttc tgcgacagcc
     3781 gcgtccgaaa gacttcgtcg ccgagtcgca ggaaattctg acaaagcgaa actacccggt
     3841 ggttctttcc gaactattgt accggttggc agaagcgaaa gaacgccagt tgaaagatta
     3901 cgagggagcc aacaagacct acttcgagta tctctcgttc tttccgcagg ggggaagggt
     3961 gctgcgggtg cgcgagcgga ttcctcaggt ctatgcgctg gcaaaagacc tgaaaaatgc
     4021 cttgcgattc aacgagaaac tgatcaacga tacccagatt cccgctgaca tgcgggttga
     4081 tgcgtcactg cgcaaagcca ggctcgaaga gaccaccgac gagaaggaag aggcggtaaa
     4141 aacgctcgaa gccgcgctca gctttgaatc aaagcgtcgt ccagaactgt atctgcagct
     4201 cgagcgtctg accagtactt tcgagtatgt gcagaaggcg cttgatacgc ccggagagga
     4261 aaccttccgc ttcgaggctc tgcaacgcct gattcgcaag gcggaagaag atcagaaaca
     4321 cgatgaggcg gcgcgtctgg tacgtgagta tcagaaggcg tttaccggag aagaggccgt
     4381 ggcctggatc gccaagaaag gtgaggatct cggcaaacgc ggcaccatcg cggaaatcga
     4441 agccaagatt gaagggttcc ctgaagagcc cgagacggcc gctcgattgt acaagttggc
     4501 gcagttggtc gaaggtatgg aaaacaccaa gtatcgctcg caggatctct tttacgagtt
     4561 gaccctggtg tatcccgggt cgccatttta tcgcgaaagc aaactgcggg cggaaaatgt
     4621 ccgggtaatc aaatcgatcg atcagattgg cggtctgctc aaaaaagggg tgaagaccgg
     4681 cgagggggat gctttgctgc tgcaccgggc acgtctctat cgtgatacgt tgcaggatac
     4741 cgcccgggcc cgcgaggatt atgaagctct gctcaagctg tttcctgatt cgccccgtct
     4801 cgacgaggcg tatcttggtc tcggctcact gatcctcaag gtggaccgcg atggagaaaa
     4861 ggcgcgtgag tactgggaaa agggtctcgg cgtctcgcgg aaccccgagg tgcggcgaca
     4921 gctggcgcgt aacattgatg atctgcggca atttcgtgaa aagatcctgt actcggagaa
     4981 gaacaccgat catgccgccg gcacggatct ggtctggaag cgggcgcgtc tcgaaaacga
     5041 ccgggacggt gctcaggcgc tggtgcagga agcgttgcgg caacttgaga accgtccgca
     5101 gcgtgctcgc ctgcactacc tgaacgggcg tctgttcgag gaggctgagc gctggggcga
     5161 ggcgatttcg gaatacgaag ctgcgcatgc ctgtctgtat catcccggct gtcgcaaaga
     5221 tcgagtattg taccggttgg ggcggttgta tgcccgcact ggaaaaatgg aagaggctta
     5281 tcgaagatgt gtcgcgttgg tgacgcgata tccgcgcagt cgcttctcgc ggtccgggtt
     5341 ctactggctc tacaaacagg atctggcggc taagcgtttc aatctggcgt ttgacagact
     5401 gggccgactc ctgcagttcc gcaatctctt tccgacgcat cggcaagcca tcaccaagtt
     5461 gcagaaagag gtcggggctc gcatgaacct ggcggacatg cagcgtctgc aggcgctggc
     5521 gaaaaccgat ggtggtgccg cttttcatta ctatatcggc cgggtgctcg agaacgacat
     5581 gggtgatgtc gatggcgcca tgcgcgaata tgaaacctat ctgcgttcga acccaccgat
     5641 cgcccgagct cgcgatctct tgatgaaact ggcgcagctg gccgaaagc
//
LOCUS       DAORHD010000110         5688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14460_length_5688_cov_4.194568, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000110 DAORHD010000000
VERSION     DAORHD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14460_length_5688_cov_4.194568"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="PKO06_03545"
     CDS             <1..1793
                     /locus_tag="PKO06_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV68745.1"
                     /translation="LCGWQEAEARGKTLSEVFRILNEETRQPVPNPAEGALRECRVIT
                     LANHTILVSRDGREFPIEDSGAPIYDTDKKICGSVLVFRDKSADVAAERERALLEYAI
                     RSSVNEIYLFDADTLRFRFVNEGALRNLGFTLDQMRTMTPLDIKPTHTPESFARLVEP
                     LLRREVPVIRFETEHQRANGSRYPVEIHLQLFEHQHRRVFLAVIQDLTERRNMEGQLH
                     QAQKMESIGNLAGGVAHDFNNMLGVIIGRAEVAQQRLPADNPVQSDLQEILQSAHRSA
                     DLTRQLLAFARKQTASPRVIDLNEVIDGMLRMLRRLIGENISLRWQPGASLWPIRIDP
                     AQIDQILANLVVNARDAIRNVGTISLETENIAFDAEYVQAHPEFVQGDFVRLSVSDTG
                     SGMTKEVMARVFEPFFTTKELGKGTGLGLAMVYGIVKQNDGFINVYSEPGRGTTFNLY
                     FGRSRETVQTPAQPVTLPSATGHETILLVEDEPSLLELGTMLLTRLGYRVLTVGSPQE
                     ALERVSTNSESIDLLITDVVMPEMNGRDLAEAIRKIRPGIRVLFMSGYTADVIAHHGV
                     IEAGKAFLQKPFNRSQLANTVRQVLDAPLS"
     gene            complement(1899..2438)
                     /locus_tag="PKO06_03550"
     CDS             complement(1899..2438)
                     /locus_tag="PKO06_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68746.1"
                     /translation="MKKVLCLMLVLCLTLPAMAMAAKGVPSALKAQDTLTADQKATFE
                     GKARVKMGKEFLFVSKDAAKASLKKDDVCTEKRQIHYVCCCQKVTKNRPGKCPCGKGL
                     IPAIKLDNNWYTLDRDELGQLVVKPFVAVDCAAKADCCGGTKADCPSCGKCAPKAEPA
                     PAADAAPAPAPADCGSCGN"
     gene            2649..4238
                     /locus_tag="PKO06_03555"
     CDS             2649..4238
                     /locus_tag="PKO06_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HNV68747.1"
                     /translation="MTTLKDEITRRRTFAIISHPDAGKTTLTEKMLLFGGAIQMAGSI
                     KARKAARYATSDWMAIERERGISVTSSVMNFCYRDIDLNLLDTPGHQDFSEDTFRVLT
                     AVDGALMVIDSAKGVETQTKKLMEVCRMRNTPIITFINKLDREGLHPLDVLQDIENTL
                     QIECAPLTWPIGMGREFRGVYDRRRHEVRLFKAGSDVRPEDVVIIRDLADPRLDELLG
                     SQAAELRHDLELLEGASSPFDFDHFRRGNQTPVFFGSALNNFGVQEVLDAVVDLAPPP
                     QPREALSRVVMPEEAPFSGFVFKIQANMDPQHRDRIAFLRVCSGRFERGLKVRHHRLG
                     KDLTIKDATMFLARQRTNVEEAFPGDIIGIHNRGTLKIGDTLSEKETLQFTGIPSFAP
                     ELFRSVRIKNPLRSKQLQIGLKQLSEEGAIQVFKPLLAGGETILGAVGALQFEVTKAR
                     LADEYQVDAVYSNVEYVTARWPTSSDKAKLDEFIRQNKQHMAEDTEGQMAYLVGNLYA
                     FGRLAEKWPMIEFRMFREYRQ"
     gene            complement(4279..5436)
                     /locus_tag="PKO06_03560"
     CDS             complement(4279..5436)
                     /locus_tag="PKO06_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV68748.1"
                     /translation="MTEHAYRGDLFGPEDPARSSAVQVAIGYDSLRIKKETGQEYSLP
                     FSSAQMSRGGFDGLGIIVQGRNEAGQTIYLQLQDEAFLRELRDRGPATVQPAVRAMLG
                     QGTATRAWGYFALIGLVVFLVGGSLALYFGSGYLAEIVVSHIPHSAEASLGKLTAESL
                     TQGKQLITEGTVNDGVQTVWKRVLSGIEKNPYTFNLSLVESSTVNALAAPGGHIVVFS
                     GLLANLESGEELAGVLAHECAHALRRHSLKRIAKTAGISIAFGLFLGDLGGLTGLFTE
                     FGKELTLLSYSRDDEREADEEAVQILVRAGIDPTIFPNFFRRMGEKESSVEKALAIVS
                     THPSFAERDQRLQTLFAAHREKTFRPIDVDWKRLREALSATAKLGQKPGQD"
     gene            complement(5438..>5688)
                     /locus_tag="PKO06_03565"
     CDS             complement(5438..>5688)
                     /locus_tag="PKO06_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNV68749.1"
                     /translation="FGGIYKISLAPGQHYVVDNGHLVAWSNTVQYEVQKASSGWLSSM
                     TSGEGFVCHVRGPGDVWFQTRNPTGFGAWVRQFIPTKG"
BASE COUNT         1210 a         1756 c         1594 g         1128 t
ORIGIN      
        1 agttgtgcgg gtggcaggaa gcggaagccc gtgggaaaac cctttccgag gtctttcgga
       61 ttctcaatga ggaaacccga caacccgtgc ccaatccggc agagggggcc ctccgggagt
      121 gccgcgtcat cacgctcgcc aatcacacga ttctggtgtc acgagacggg cgggagtttc
      181 cgatcgaaga cagcggtgcg ccaatctacg atacggacaa gaagatctgc ggctcggtgc
      241 tggtgtttcg cgacaagtcc gccgatgtgg ctgccgaacg cgaacgagcg ctgctggaat
      301 atgccatccg ttcgagcgtc aatgaaatct acctgttcga tgccgacacg ctcaggttcc
      361 gcttcgtcaa tgaaggcgcg ctgcgcaatc tcggctttac gctcgatcag atgcgcacca
      421 tgaccccgct cgacatcaag ccgacgcaca ctcccgagtc ttttgcccgt ctcgttgaac
      481 cgctgctgcg acgtgaagtt cccgtgattc gtttcgaaac cgagcatcaa cgagcgaatg
      541 gctcccgcta ccccgtcgaa attcatctac agctgttcga acatcagcac agacgggtgt
      601 ttctcgccgt cattcaggat ctcacggaac gccgaaacat ggaggggcag ctgcatcagg
      661 cacagaaaat ggaatccatc ggcaatctgg cggggggggt cgcgcacgac ttcaacaaca
      721 tgctcggggt catcatcggg cgagccgaag tcgcgcagca acgactgcct gccgacaacc
      781 cggttcagag cgatcttcag gagattctcc aatcggcgca tcgctcagcc gatctgactc
      841 gccaacttct ggcgttcgcc cgcaagcaga ccgcttctcc ccgcgtcatc gatctcaacg
      901 aagtcatcga cggcatgctg cgcatgctgc gtcgactgat cggagaaaac atctcgctgc
      961 ggtggcaacc aggtgccagc ctctggccaa ttcgcatcga cccggcacag atcgatcaga
     1021 ttctggccaa tctcgtggtc aatgcgcgcg acgcgattcg aaacgtggga accatctcac
     1081 tggaaacgga aaacatcgcc ttcgatgccg agtatgtaca agcacacccg gagtttgtcc
     1141 agggtgattt tgtccgactt tcggtcagcg acacgggttc aggtatgacg aaagaggtaa
     1201 tggcccgtgt cttcgaacct ttcttcacga ccaaggaact ggggaaaggc accggtctcg
     1261 ggctggccat ggtctacggc atcgtcaagc agaacgatgg attcatcaat gtttacagcg
     1321 agccggggcg cggcacgacc ttcaaccttt acttcggtcg atctcgcgag accgtgcaga
     1381 caccagctca gccagtgacg ctaccatcag cgaccggaca cgaaaccatt ctgctggtgg
     1441 aagacgaacc cagtctgctg gaactcggca ccatgctgct gacgaggctc gggtatcggg
     1501 tactgaccgt cgggtcgccc caggaagccc ttgaacgggt atcgacgaac tccgagtcga
     1561 tcgatctgct gatcaccgac gtcgtcatgc ccgagatgaa cggccgcgat ctggcggagg
     1621 ctatccgaaa gattcgaccc gggatccgcg ttctcttcat gtcgggctat acggcggatg
     1681 tcatcgccca tcatggggtc atcgaggccg gaaaagcctt cttgcagaag ccgttcaatc
     1741 gaagccaact ggccaacacg gtgcggcagg tgctggacgc gccgctcagc tgatcactca
     1801 cgttcagcca cacacacccg ccgttgcctg atcgaccttt tcagccgaaa tgaaaaacag
     1861 gtccggaaat ccggacctgt ttttctacgg aaaaagactt agttgccgca ggaaccgcag
     1921 tcagctggcg caggagcagg agctgcatcg gctgccggag cgggttcagc cttcggagcg
     1981 cacttgccgc aggaagggca gtcagccttg gtcccaccgc agcagtcagc tttcgctgcg
     2041 caatcaacgg ccacgaaagg cttcaccacc aactggccga gttcatcgcg gtccagggtg
     2101 taccagttgt tatccagctt gatggcggga atcaggccct tgccgcaggg acatttgccc
     2161 gggcggttct tggtgacctt ctggcagcag cagacataat gaatctggcg cttttcggtg
     2221 caaacgtcgt ctttcttcag cgaagccttc gcggcatctt tgctgacgaa gaggaattcc
     2281 ttgcccatct tcacgcgggc cttgccctca aacgtcgcct tctgatcggc tgtcagggtg
     2341 tcctgagcct tcaacgcgga aggaacgcct ttggcagcca tggccatcgc gggcagcgtc
     2401 agacagagaa ccagcatcag acaaagcact ttcttcatgt gtataccatc tcccattaca
     2461 gtttaaattg gcgattttct cacctgccga aagtctaccc tacgattccg gtaccagcaa
     2521 ctttttttcg tccacagcat cctgtactgt ttctctccag tctcgtcatt cgctctcagc
     2581 attcgcggtt gcgagtcgcg acaaagcgtg gtacaacttg gttccgcaat ttttcgtgga
     2641 gaccagccgt gacgacactg aaagacgaaa tcacccgacg acgcaccttt gccatcatca
     2701 gtcaccctga cgccggcaag accaccctga ccgaaaaaat gctgctgttc ggcggcgcca
     2761 ttcaaatggc cgggtccatc aaagcccgca aagccgctcg ctatgccacc tccgactgga
     2821 tggccatcga gcgtgagcgt ggcatctcgg tcaccagttc ggtcatgaac ttctgttatc
     2881 gggatatcga tctgaacctg ctcgacacgc ccgggcacca ggacttttcc gaagacacct
     2941 ttcgcgtgct caccgccgtc gacggggcgc taatggtcat cgacagcgcc aagggcgtcg
     3001 agacccagac caagaaactg atggaagtgt gtcgcatgcg caacacgccg atcatcacct
     3061 tcatcaacaa actcgaccgc gaagggcttc acccgctcga tgtactgcag gatattgaaa
     3121 acacgttgca gatcgaatgc gcgccgctga cctggccaat cggcatgggc cgagagtttc
     3181 gcggcgtcta tgaccggcgg cggcacgagg tgcgtctgtt caaggccggc agcgatgtgc
     3241 gaccggaaga cgtcgtcatc attcgcgatc ttgccgaccc gcgtctcgac gaactactcg
     3301 gcagccaggc cgccgagctg cgccacgatc tcgaactgct cgagggtgct tcgagcccct
     3361 tcgacttcga tcacttccgg cgcggcaatc agaccccggt ctttttcggc agcgcgctga
     3421 acaatttcgg agtccaggaa gtcctcgacg cagtcgttga tctggccccg ccaccacagc
     3481 ctcgcgaggc actttcccgc gtcgtcatgc ccgaggaagc gccgttttcg ggcttcgttt
     3541 tcaagattca ggccaacatg gatccgcagc accgcgatcg tatcgcgttt ctgcgggtct
     3601 gctcaggtcg tttcgaacgc gggctgaagg tgcgccatca tcgtctcggc aaagacctga
     3661 ccatcaagga cgccacgatg tttctcgccc gccagcgcac caacgtcgaa gaagcgtttc
     3721 cgggcgacat catcggcatt cacaatcgcg gcaccctcaa gatcggcgac acgctctccg
     3781 aaaaagagac gttgcagttc accggcattc ccagcttcgc gcccgaactg ttccggtcgg
     3841 tgcgcatcaa gaacccgctg cgctccaagc agctgcagat cggtctcaag cagctctcag
     3901 aagagggtgc gattcaggtg ttcaagccgc tgctcgccgg cggtgaaacg attctcggcg
     3961 cggtcggcgc gctgcagttc gaggtcacca aggcacgtct ggccgacgag tatcaggtcg
     4021 acgccgtcta ttcgaatgtc gagtatgtga ccgcccgctg gccgaccagc agcgacaagg
     4081 cgaaactcga tgaattcatc cgtcagaaca aacagcacat ggccgaagac accgaagggc
     4141 agatggccta cctggttggc aacttgtacg cattcggtcg tctggccgag aaatggccga
     4201 tgatcgaatt ccgcatgttc cgcgagtatc ggcagtaacg aaggtcgcgc aagtgtcgcc
     4261 ggggtcaccg gcgccggttc agtcctgtcc cggtttctgt ccgagtttgg ctgttgccga
     4321 aagcgcttcg cgaagccgtt tccagtctac gtcgatcggg cggaaggtct tttctcgatg
     4381 cgccgcgaac agggtctgca gtcgctgatc tctttccgcg aaggacggat gcgtcgagac
     4441 gatcgccagg gctttctcga ccgaactttc cttttctccc atgcgtcgaa agaagttcgg
     4501 aaagatcgtg ggatcaatgc cggcacgcac cagaatttgt accgcctctt catcggcctc
     4561 gcgttcatcg tcgcggctgt aggagagcaa ggtcagttcc ttgccgaact cggtgaacag
     4621 gccggtcagc ccgcccagat caccaaggaa caaaccgaag gcgatgctga tgccggcggt
     4681 cttggcgatg cgcttcaggg aatggcgccg cagggcatgc gcgcactcgt gtgccagcac
     4741 tcccgcgagt tcttcaccac tctcgagatt cgccagcagg ccggaaaaga ccacgatgtg
     4801 ccctcccggt gccgccagcg cattgacggt cgacgattcg accagagaga gattgaaggt
     4861 atacggattc ttctcgatac ccgaaagcac gcgcttccag accgtctgca ccccgtcgtt
     4921 gaccgttcct tccgtgatca gctgcttccc ctgtgtgagc gattccgcgg tcagctttcc
     4981 cagactggct tcggctgagt gagggatgtg tgagacgacg atctcagcga gatagcccga
     5041 accgaaatac agtgccagac tgccacccac cagaaagaca accaacccga tcagggcaaa
     5101 gtagccccac gcgcgggttg ccgttccctg cccgagcatc gcccgcacgg cgggctgcac
     5161 cgtggccgga ccacgatcac gcagttcgcg cagaaacgct tcgtcctgca gttgcagata
     5221 gatcgtctga ccggcttcat ttcggccctg aacgatgatc cccagaccgt cgaagccgcc
     5281 gcgactcatc tgagccgaac tgaagggaag actatattcc tgaccggttt ctttcttgat
     5341 tcgaagagaa tcatagccaa tagcaacctg gacggcagaa cttcgggccg gatcctccgg
     5401 cccgaagaga tcgccacgat aggcgtgttc cgtcatatca ccctttggtc ggaatgaact
     5461 gtcggaccca ggcaccaaaa ccggtcgggt tgcgggtctg gaaccagaca tcaccgggac
     5521 cgcgcacgtg acagacaaag ccttcgcccg aggtcatcga ggacagccag ccgctcgacg
     5581 ctttctgcac ctcgtactgc acagtgttgc tccaggcaac gagatgcccg ttgtcgacaa
     5641 cgtaatgctg accgggggcc agactgatct tgtagatgcc accgaaac
//
LOCUS       DAORHD010000111         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14494_length_5678_cov_3.484972, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000111 DAORHD010000000
VERSION     DAORHD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14494_length_5678_cov_3.484972"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PKO06_03570"
     CDS             complement(<1..508)
                     /locus_tag="PKO06_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68750.1"
                     /translation="METSELIEIISRGEDGKNQFKADARNNTQLAAEMVAFCNSGGGR
                     LFLGVSDNGSVSGLQREDVSRLNLLISDAATNLVKPSINPETENVRHPDGIVIVVKIP
                     DGLSKPYMDNAGAIWVKSGSDKRKVTSREEIQRMLQKSGLIHGDEVPAHGVSIASIDM
                     DFFSAFYEK"
     gene            complement(490..3156)
                     /locus_tag="PKO06_03575"
     CDS             complement(490..3156)
                     /locus_tag="PKO06_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HNV68751.1"
                     /translation="MNKNDQEPLERMPQIFAHGSRWVRADFHLHTKADKQFAYAGEEN
                     SYLSAYVDALANAEIRVGVIANHNKFVKNEFDAMRKNARKRDIYLLPGVELSVADGAN
                     GIHTIVVFSEAWLENGQDYIYQFLNVTFAGKTPDQYENENGRSQDSLSETIKKLESYN
                     RDFFLIFAHVEQNSGLWLELDGGRLAEVGKTDYFQRRCRGFQKVRTHDKQNAPCRMKV
                     KEWLGASYPAEVEGSDPKRIDEIGKGEACYVKIGAFSFEAVKFALCNFENRVAREVPR
                     HARSHIKSIRFEGGTLNGQDLSFSPGLNALIGVRGSGKSSIIEAIRFAMDIPAGDNPA
                     DDEYKRKLIKHTMGSGGKVTVIAVDRFGQEYEIRRIWDHRPEVLVNGELQPSGLSIRE
                     AVVHKPLYFGQKDLSNSGEGFEKNLVEKLIGEKSREIRLQIAEQQQKVQESIRRYLKL
                     SNISARKEEHTSKKQNAEFQLERYKKYGLEEKLQRQVDFDADSRKLREIAESVTAFHD
                     ALTDLLMQHEDAIRNHVLYKSRQNETYFNSYFSAYASVVTQMDALKQSRDSLAESRAS
                     LAGKQAEFDQIFQGLKEEFAAIERSISDELREAGVPTIRPDEFRQLNKVLEQSRAILA
                     ELDKEQAKSEEIRQELTRELSLLNELWHKEFQIIQDEMGKVNANHSSLEIVTDFKADK
                     AAFKVFLREILKGSALRDTTIQKLVDEFIDMHAIFEERERAEQILAESGPKFWEYFFK
                     QLETLLIWRVPDKFTIKYGGKELKEHSLGQRASALILFVLSQRENDLILIDQPEDDLD
                     NQTIYKDVIKLIHTLKRETQFIFATHNPNFPVLGDAEMVIACDYSNNRVDPYHRSIDD
                     PLMQARIVNIMEGGKEAFERRKEIYSIWKPLS"
     gene            complement(3211..4254)
                     /locus_tag="PKO06_03580"
     CDS             complement(3211..4254)
                     /locus_tag="PKO06_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV68752.1"
                     /translation="MSDPFFDHPILNSPYEAPCRHWELDAEGQPTQRIIDERRKAEFI
                     TPIPKPKKRKGETAAQSAFVFDEGKGLSTDGQQYDPTSVINQVRSHVNAWRELPRPSQ
                     WQVTPETARLLQHWRSHQFGGIRPFFCQVEAVETAIWLTLGIDLRGRSRTLRVNYRTS
                     HQIRQQADRLLGKVMTDIDGNSEDRGDTVSLFNGPPPQILSAKSEAREISAVAQWLTE
                     KTRSGVLPHETGVFVRSTAQLSRAQKAVQEAGLKFSVLDEHVEPKHDHVSVCTMHLAK
                     GLEFRAVAVMACDDEVIPMQERIEQVGDEGDLQEVYDTERHLLYVACTRARDFLLVSS
                     VEPASEFVDDLRG"
     gene            complement(4449..>5678)
                     /locus_tag="PKO06_03585"
     CDS             complement(4449..>5678)
                     /locus_tag="PKO06_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68753.1"
                     /translation="FMKRFLPDFARQPPFSLRVNAFPEKHFMPEVFLFPEDVGRARDV
                     AREVFDTSVTVRKVQASGTEEVLVIGFRGQNLSKYVLVAQTSLEPVKQHIYSLWITLL
                     AVALLVLLSPLVIGVLLSSQILRPIGSLSEGMVAIQNRQFGHRVPVLTRDELGEMSGL
                     MNQVIEGMGDLQVARIVQESLFPPTALDMEGFRITGSSRAMADIGGDYYDYFQISPDK
                     LSGLIGDVSGHGVSAALIMGMAKCAVTLDESPDRSLVDILGQFNRFLIRTIKRKKMMT
                     GFFFTLDVKTGLLTYANAGHNFPFLYSAGSGSIRDLEMASFPLGVRAKAEFKTDTVVL
                     QPGDAILLYTDGLVESPGGPQGEGVGYELARDWFKQAAHLPPEGVLEQILASFDTYTL
                     GRPPADDVTLVCLKRLR"
BASE COUNT         1247 a         1604 c         1424 g         1403 t
ORIGIN      
        1 ccttttcata aaatgcggaa aagaagtcca tgtcaatgct ggcaatggaa acgccatgtg
       61 caggtacttc atcaccgtgg atcaatccag atttctgaag catccgttga atttcttcac
      121 gagacgttac ttttcgtttg tcagaacccg acttgaccca aatcgctccc gcattgtcca
      181 tatatggttt gctaagcccg tcaggaatct ttacgacaat gacaattccg tcggggtgtc
      241 tgacattctc tgtttccggg ttgatcgacg gctttacaag attggttgcg gcatcggaaa
      301 tgagaagatt caatcgtgaa acatcctctc tctggaggcc ggaaacggag ccattatcac
      361 tgactccgag gaaaagccgc ccgccaccag aattgcagaa agccaccatt tcagctgcta
      421 actgggtatt gttcctcgca tcggctttga actgattctt gccgtcctca ccccgactga
      481 tgatttcaat taactcagag gtttccatat gctataaatc tccttgcggc gctcgaacgc
      541 ctccttgcca ccttccatga tgttgacgat tctggcctgc atgagcggat catcgatact
      601 gcggtggtac ggatcgaccc gattgttcga gtaatcgcag gcgatgacca tctccgcatc
      661 tccgaggacc ggaaagtttg ggttatgcgt ggcgaagatg aactgcgtct ctcgcttcaa
      721 tgtgtgtatg agtttgatca cgtctttgta gatagtctgg ttatcgagat catcttcagg
      781 ttggtcgata agaatgagat cgttctcacg ctggctcaga acgaacagaa tcaaagccga
      841 cgcccgctgt cccaaggaat gctccttgag ctccttgcct ccatatttaa tggtgaattt
      901 gtctggaacc cgccagatca acaaggtttc aagctgtttg aaaaaatact cccaaaactt
      961 cgggccactt tccgccagta tctgctcggc ccgttccctc tcctcgaaga ttgcatgcat
     1021 atcaatgaac tcatcaacga gcttctgtat cgtcgtgtct cgaagagcgc tccctttgag
     1081 aatttcacgc agaaacacct tgaaagccgc tttgtcagcc ttgaaatccg tcacaatttc
     1141 gagcgatgaa tggttcgcat taaccttacc catttcatcc tgaatgatct gaaactcttt
     1201 gtgccataac tcgttcaaga gggaaagctc tcgcgtcagc tcctgtcgaa tctcctccga
     1261 cttcgcctgt tctttgtcga gttcggcaag aatcgcccga gattgctcca acaccttatt
     1321 cagttgtctg aattcatcag gtcgaatggt cggaacgccg gcttcacgta actcatcgga
     1381 gatgcttcgc tcaatggcgg cgaactcttc cttgaggcct tggaagatct gatcaaactc
     1441 cgcttgtttc ccggccaggc tcgctcgaga ttcggcgaga gaatctcggc tctgcttcaa
     1501 cgcatccatc tgtgtcacaa cagaagcata agccgagaag tagctgttaa aatacgtttc
     1561 attctgccta gacttgtaca acacgtgatt tcttatcgcg tcttcatgtt gcatcaacag
     1621 gtcggtcaac gcatcatgaa acgcagtcac actctctgcg atttccctga gctttcggga
     1681 atcagcatcg aaatcgacct gtctttgaag cttttcctca agcccgtact tcttgtatcg
     1741 ctctagctga aactcggcat tctgcttctt ggaagtgtgc tcttccttgc gagcagaaat
     1801 gttcgagagc ttcagatacc gacgaatgga ttcctgaacc ttctgctgct gttctgctat
     1861 ctgcagtctg atttcacgcg atttctcgcc gatcagtttc tctaccaggt ttttttcgaa
     1921 cccttccccg gaattcgaca ggtccttctg gccgaaatac aaaggcttgt gaacgacggc
     1981 ttcgcgaatc gagagccctg aaggctgaag ctcgccattc acgagcactt caggccgatg
     2041 atcccagatg cgcctgatct catattcctg gccgaaacga tcgaccgcaa tgaccgtgac
     2101 ttttccgccg ctccccatag tgtgtttgat gagcttgcgt ttgtactcat catctgcagg
     2161 gttgtcaccg gctggaatat ccatggcaaa gcgtattgcc tcaataatcg atgattttcc
     2221 acttccacgt accccgatca gggcgttgag tcccggggag aaacttagat cctgaccgtt
     2281 gagcgtacca ccctcgaatc gaatcgattt gatatgtgac cgagcatgcc tggggacctc
     2341 acgcgccacc cgattctcga aattgcacag ggcaaacttc accgcttcaa aggaaaaagc
     2401 accgatcttc acgtagcacg cctcaccctt gccgatctca tcgatcctct tggggtccga
     2461 cccctcgacc tcggcagggt aagaagcacc gagccattct ttgaccttca ttcggcaagg
     2521 agcgttctgc ttgtcatggg tacgaacttt ttggaaccca cggcatcgtc gctggaagta
     2581 atcggtcttt cccacctccg ctaaccgacc accatccaat tccagccaga ggccgctgtt
     2641 ctgctcgaca tgagcgaaga tgaggaaaaa atcccgattg tacgattcga gcttcttgat
     2701 cgtctcactc aggctgtcct ggcttcgccc attctcgttt tcgtactgat cgggggtttt
     2761 tccggcaaag gtaacgttca ggaactgata gatgtagtct tgcccgtttt cgagccacgc
     2821 ttcactgaag acgacaatgg tatgaatgcc gttcgcccca tccgcgaccg acagttcaac
     2881 cccaggcagc aagtagatgt ctcgctttcg ggcattcttg cgcatcgcat cgaattcgtt
     2941 cttcacgaat ttgttgtggt tggcaatgac gcccacacgg atttccgcat tggccaatgc
     3001 atccacataa gcgctcagat aggaattctc ttcaccagca taagcgaact gcttatcggc
     3061 tttcgtatgc agatgaaagt cggccctgac ccaacgagag ccgtgcgcaa agatctgtgg
     3121 catccgttca agaggttctt gatcgttttt gttcatgctg aattctacct tatttcttcg
     3181 gcatccgtgt catatgcacg acgagtcggt tcaaccgcgc agatcgtcga cgaattcgga
     3241 tgccggttcg acactgctga ccagcaggaa gtcacgggcg cgggtgcagg cgacatacag
     3301 aagatgtcgc tccgtgtcgt agacctcttg taaatcaccc tcatcgccga cctgctcaat
     3361 gcgttcctgc atcggaatga cctcgtcgtc acaggccatg accgccactg ccctgaattc
     3421 gagccctttg gcgaggtgca tggtgcaaac cgaaacgtga tcgtgtttcg gctccacgtg
     3481 ctcgtcgagc acggagaact tcaaccctgc ctcttgaacc gccttctgag cacgtgaaag
     3541 ctgagcggtc gatcggacaa aaacacccgt ttcatgcggc aacacccccg aacgggtttt
     3601 ctctgtcagc cactgcgcta ccgcactgat ttctcgtgct tcgcttttcg ccgaaagaat
     3661 ctgaggcggt gggccgttga acagcgatac cgtgtctcct cgatcctcgc tgttgccgtc
     3721 gatgtccgtc atgaccttgc cgagcaggcg atcggcctgc tgacgaatct ggtgtgatgt
     3781 gcgatagttg acgcgcaacg tccgcgaacg gccgcgcaga tcgataccca gggtcagcca
     3841 gatcgctgtt tcaacggctt caacctgaca gaagaagggg cgtatccctc cgaactgatg
     3901 actgcgccaa tgctgcaaga gtctggcggt ttcaggggtc acctgccact ggctcggccg
     3961 aggcagttca cgccaggcgt tgacgtgact tcgcacttga ttgatgactg aggtcggatc
     4021 atactgctga ccgtcagtcg acagtccttt tccttcatca aacacgaagg ctgactgagc
     4081 ggctgtttcg cccttgcgtt tcttcggctt gggaatcggc gtgatgaatt ccgcttttcg
     4141 ccgttcgtca atgatgcgct gggtcggttg gccttcggcg tccagttccc aatggcgaca
     4201 aggggcttca tacggtgaat tcaaaatcgg gtgatcgaaa aacggatcag acatgttgct
     4261 tctctcgtca gttcttccgt cggttttcac ggcagtctca cattcccgct cagctgaccc
     4321 cacaggtttt cacattgctt caatcgacga tgcactcacg gtgtcgaaaa tctgacgacc
     4381 gtaagcctgt atgggcatgt cgttaacgat accgcaggtg ctcagtttag acaagtagat
     4441 gccatggctc agcgcaggcg tttgaggcag accagagtga cgtcatcggc gggggggcga
     4501 cccagcgtgt atgtgtcgaa cgaagccagg atctgctcga gcactccttc gggcggcaga
     4561 tgcgctgcct gcttgaacca gtcgcgtgcc agttcgtacc cgaccccttc gccctgcggt
     4621 ccaccaggtg attcgaccag gccgtcggtg tagagcagaa tggcatcgcc gggctgcagc
     4681 accacggtgt cggtcttgaa ctcggccttg gcccgcactc ccaaagggaa cgatgccatt
     4741 tcgagatcac gaatcgaacc gctgcctgcg ctatacagga aggggaagtt gtgtcctgcg
     4801 ttggcatagg tcaacagacc ggttttcacg tcgagggtga agaagaaccc ggtcatcatc
     4861 ttttttcgtt tgatcgtacg aatcagaaaa cggttgaact ggcccagaat gtcgaccagc
     4921 gatcgatcgg gagattcgtc cagcgtcacc gcgcacttgg ccatgcccat gatcagcgcc
     4981 gctgagacac cgtgtcccga cacgtcgccg atcagcccgc tcagtttgtc cgggctgatc
     5041 tgaaagtaat cgtagtagtc gccgccgata tcggccatgg cgcggctgct accggtgatg
     5101 cgaaatcctt ccatatcgag cgccgtggga gggaagagac tttcctgcac gattcgtgct
     5161 acctgaaggt cgcccatacc ctcgatcacc tgattcatca acccggacat ttcacccaac
     5221 tcgtcacgag tcagaaccgg aacgcgatga ccgaactgac ggttctgaat cgccaccatt
     5281 ccttcgctga ggctgccgat cgggcgtaga atctggctcg aaaggagcac gccgatcacg
     5341 agtggcgaga gcagcaccag cagtgccacc gccaacagcg tgatccagag actgtagatg
     5401 tgttgtttga ccggttcgag cgaggtctgt gccaccaaca cgtacttgct caggttttgt
     5461 ccgcgaaagc ctataaccag cacttcttct gttccagaag cctggacttt tcgcaccgtc
     5521 acactggtgt cgaacacttc acgggcaaca tcgcgggccc gacccacgtc ttccggaaag
     5581 agaaaaacct cgggcatgaa gtgcttttcc ggaaaggcgt tcacccgaag agagaagggc
     5641 ggttgtcggg cgaaatcggg cagaaatcgc ttcatgaa
//
LOCUS       DAORHD010000112         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14507_length_5673_cov_3.224635, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000112 DAORHD010000000
VERSION     DAORHD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14507_length_5673_cov_3.224635"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1223
                     /locus_tag="PKO06_03590"
     CDS             135..1223
                     /locus_tag="PKO06_03590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV68754.1"
                     /translation="MDHHGNPLIVETWPEGPIAADPPLPWDVYWEAAWARAWQVETGG
                     QPTMFVYRASNHVFVHSFLVHSIDGNDGVDLETPYGYGGPLANTQDPEFLTTAWRAFA
                     TWAHDRRVVTEFVRFHPLIENTRFVADPDLKISVDRETIWYDGSAGAPTSGSETNAVP
                     SAAFRRGVAHALKSGLRFAELPIDSCYELFRSLYRDTMIRLHADQFYLFSDRHWDALR
                     AASIKIRLFGVLADDRLAAAALCLEHTPWLHYHLGASDASLLDRRPNNLLFHGLQEAA
                     RRMPGIRAVHFGGGSDASPDNSLLRFKAGAGGRRARFSIGRRIWDPNAYARLKSEVLR
                     RHPELRQYENTYLQIHRLIPRPTREEHS"
     gene            1220..2440
                     /locus_tag="PKO06_03595"
     CDS             1220..2440
                     /locus_tag="PKO06_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNV68755.1"
                     /translation="MTPKPATVTALPKPLVRAEALAEELIVLRRGFHAHPEIAFQEIE
                     TAAKIAKTLTDWGLTRVRTGVGKTGICVDIGSASGRLVALRADMDALPIQEELSKPYA
                     SQVPNVMHACGHDAHIAMALGAAKLLHDRHREQPLPGRVRVLFQPAEEHQDAEGLSGA
                     PRMITDGALDDVAAIFGLHVDATRPLGFVRVQPGPYSAAVDTFRATIIAQGGHGAYPH
                     DGRDPLWMLIPILSALHAIVSRRVNPLEQAVVSLGQIHGGTASNILPAEVHLEGTLRS
                     FNPAVRETLIDEVRAALGLSRSLGGDFRLHVQRGYPSTRNDSAMTTFFESVLADLLEP
                     GLVHHEPMGMGGEDFAYYCEKVPGVFAMIGAAIADGKLRPHHTPVFDIDERILPLGAA
                     ILAESALRFLNRPG"
     gene            2546..3616
                     /locus_tag="PKO06_03600"
     CDS             2546..3616
                     /locus_tag="PKO06_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV68756.1"
                     /translation="MSHWRNILIKPETSILDTLRVIDDSSLAIALVVDHEEKLIGTVT
                     DGDIRRALLKGHPLTLPAREIMNLHPISAGQHTPEEELLFMLSRHSIKHLPVTDSAGR
                     VVGLRQLQDLIQRQQRENWAVIMAGGRGTRLGELTRSTPKPLLTVGDRPLLGTILTRL
                     RRHGIRRVFLAVNHLADQVKTYVGDGSAFDLEVSCLEEREVLGTAGALGLLPEKPALP
                     LIVMNGDLLTSVHFGNLLDFHTASHRALTVCIRECRFQVPYGVVTARGNDLIDIIEKP
                     EQEVFINAGIYILNPEVLERIVPGTKIDMPDLIKKMTGEKGGVGCFPLGSFWMDIGRP
                     DDFLRAQSEYQQHFSESTEVRP"
     gene            3613..4617
                     /locus_tag="PKO06_03605"
     CDS             3613..4617
                     /locus_tag="PKO06_03605"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV68757.1"
                     /translation="MNWQGKKVAVTGAGGFIASHLVETLVRQGAQVRALVRYTSRSDN
                     GWLDDVRRNLGTEFEIVRGDIRESQGIMRVVEGCDYVFHLAALIAIPYSYDWPGSYIE
                     TNVVGGFNVLEAARAQRVSKVIFTSTSEVYGSALYTPMDETHPLQTQSPYAASKAASD
                     LLAASYARSFEMPVVVVRPFNTYGPRQSRRAVIPTIISQALVGTEIYLGDTTPVRDLN
                     FVADTVAGFLACAANAVADGRVYNLASGVGVSIGEVVERIGRLLGKPLTIVSDPRRRR
                     PEASEVRCLIGASKRVEAECGWKPTFSLDEGLRQTVSWFAQHPEIRSAATESVREYVK
                     "
     gene            4614..>5673
                     /locus_tag="PKO06_03610"
     CDS             4614..>5673
                     /locus_tag="PKO06_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV68758.1"
                     /translation="MTTERPGFIPNCVPVVRGREAEYVQECLKTGWVSSVGPFVDRFE
                     REFAAYVGARHAVATCNGTAALHLALLCAGVKPGDRVLVPTLTFIATVNPVRYCGAEP
                     LLVDAEPTTANVDPRQILEICERESCAGRRIAAILPAHLYGHPAHLEPILAFARQKNI
                     PVIEDASECLGGRLGERHVGTCGLIGCFSFNGNKLITSGGGGGMLVTDDATLAARAKH
                     LSVQARSDESEYLHDAVGYNYRLTNIHAAFGCAQLEQIETVLAAKRRIHDWYERELQG
                     VPGLTLLKEQPWARSSHWMSNVLVEPGVFGADRRDLLRYLASIGIQTRPFFTPMHRLS
                     MYRASEDAFPVAERLYQQG"
BASE COUNT         1078 a         1665 c         1740 g         1190 t
ORIGIN      
        1 gggccttatg ctggggaagg ggtgacgcac tttgccatcg aagacgaagt gagagactgg
       61 ctgaagcctt tcagggaatt cgcactcgaa atgagtgtgc gctcgcatga gaatcgaacc
      121 cgcgaggtgc gtcattggat catcacggca acccgctgat tgtggagacc tggcctgagg
      181 ggccgatcgc ggcagaccct ccgttgccgt gggatgtcta ttgggaagcg gcatgggcgc
      241 gtgcctggca ggtcgaaacg ggtgggcaac cgacgatgtt cgtgtatcgc gcttcaaatc
      301 atgtgttcgt ccacagcttt ctcgtacact ccattgacgg taacgatggc gtcgatctcg
      361 agacccccta cggatacggc ggccctctgg caaacacgca agaccccgag tttctgacga
      421 cggcctggcg cgccttcgcg acgtgggcac atgatcggcg cgtggtgacc gagttcgtcc
      481 gctttcatcc gctgatcgaa aacacaaggt ttgtcgccga ccctgatctg aaaatcagcg
      541 ttgatcgcga aaccatctgg tatgatggca gtgcaggtgc gcccacgagc ggtagcgaaa
      601 ccaacgcggt cccctcggcg gcctttcggc gcggcgtcgc tcacgcgctg aagtccgggt
      661 tgagatttgc agaactgccg attgattcct gttacgagtt gtttcgatct ctctatcgtg
      721 ataccatgat ccgtttgcat gctgatcaat tctacctgtt ctctgatcgt cactgggacg
      781 cgttgcgcgc ggcctcaatc aaaatcagac tgttcggggt tctggcggac gatcgcctgg
      841 ctgccgccgc gctctgcctg gaacatacgc cttggctgca ctaccatctc ggagcttctg
      901 atgcctcgct gcttgaccgg cgtccgaata atctgctgtt ccatggcctt caggaagctg
      961 cccgccgcat gccggggatt cgagccgtgc atttcggcgg ggggagcgat gcgtctcccg
     1021 acaactccct gctgcggttc aaggccggag ctggcgggcg ccgcgcccgc ttttccatcg
     1081 gacgtcgcat ctgggacccg aacgcctatg cgcgactgaa gtcggaagtc ttgcgtcgcc
     1141 acccggaact gaggcagtat gagaacacct atttgcagat acatcgcctg ataccgcgac
     1201 caacacgaga ggagcactca tgactccgaa acctgcgacc gtgaccgcac tcccgaaacc
     1261 cctggtgcgc gctgaagccc tggcggaaga actgatcgtc ctgcggcgtg gctttcatgc
     1321 tcatccggag atcgcctttc aggagatcga aacggccgcg aagatagcga agaccctcac
     1381 ggactgggga ctgacgcggg tgcgtaccgg tgtcggcaaa accgggatct gcgtcgatat
     1441 cggctcagcg tccggccggc tggtggcgtt gcgcgcggac atggatgcgc tgccgattca
     1501 ggaggagtta tcgaagccgt atgcctcgca ggtgccgaac gtcatgcatg cctgcggaca
     1561 cgatgcgcac atcgccatgg cactcggtgc cgcgaaactg ctgcacgatc gacatcgcga
     1621 acaacccttg ccgggccgcg ttcgcgtgct gtttcagccg gccgaggaac atcaggacgc
     1681 ggaaggcctc agcggagctc cgcgcatgat cacggacggg gctctcgacg acgtggcggc
     1741 cattttcggt ctgcatgtcg atgcgacccg cccgctcggc tttgtgcggg tgcagcctgg
     1801 tccctacagt gcggccgtcg acacctttcg ggcgacgatt atcgcccagg gcggccacgg
     1861 cgcatatccg cacgatgggc gagatcccct ctggatgctg attcccattc tcagcgcgct
     1921 gcatgcgatc gtcagccgac gcgtgaatcc gctcgaacag gcggtcgtca gtcttggaca
     1981 gattcatggc ggcacggcca gcaacatcct tcctgccgag gtgcatctgg aaggcacgtt
     2041 gcgaagcttc aaccccgccg tccgcgaaac cctcatcgat gaagtgcgcg ccgctctggg
     2101 gctcagccgt tcgctgggcg gtgacttcag actgcatgtg cagcgcggct atccgtcgac
     2161 gcgcaacgac tcggccatga ccacattctt cgaatcagta ctggctgatc tgctcgaacc
     2221 gggactggta catcatgagc cgatgggaat gggcggcgag gatttcgcct actattgcga
     2281 gaaggtgccc ggtgtatttg ccatgatcgg agcagccatc gccgatggca agctgcgccc
     2341 tcatcatacg cccgtctttg acattgatga gcggatcctg cctcttggcg ctgccattct
     2401 cgccgaatca gccttgcgct ttctgaaccg cccgggttga tctcgctcgc agagttgaac
     2461 catgattcgt tcgagagtag actggtcggc gaagaatacc atcgtgattc tgcctgatat
     2521 tgggagaggc tgaccacagc tgcgaatgtc tcattggcgt aacattctga tcaagcccga
     2581 gacctcgatt ctcgacaccc tgcgagtgat cgacgattcg tcgctggcga tcgccctggt
     2641 cgtcgatcat gaagaaaagt tgatcggcac cgtgaccgac ggcgacattc ggagagccct
     2701 gctgaaaggc cacccgctga cgctgcccgc gcgggagatc atgaatcttc atccgatcag
     2761 tgccggccag cataccccgg aagaagaact gctgttcatg ttgtcacgac actcgatcaa
     2821 gcatctgccg gtgacggatt ccgccggtcg ggtcgtcggg ctgcgccagc tccaggatct
     2881 cattcaacgg cagcagcgcg agaactgggc cgtgatcatg gccggtggtc ggggaactcg
     2941 tctgggagaa ctgacgcgct cgactcccaa accgttgtta acggtcggtg atcgcccgct
     3001 gctgggtacg attctcaccc ggttgcgtcg ccatggcatt cggcgtgtgt ttttggccgt
     3061 caaccatctt gctgatcagg tgaagaccta cgttggagac ggcagtgcgt tcgatctcga
     3121 ggtgtcctgc ctcgaagaac gggaagtgct gggcacggct ggagctcttg ggttgttgcc
     3181 cgagaaaccg gctctgccgc ttattgtcat gaacggcgac ctgctcacct cggtgcattt
     3241 cggcaatctg ctcgactttc ataccgcgtc gcatcgcgcg ctgacagtgt gcattcgcga
     3301 atgccggttt caggtgccgt atggagtcgt caccgcccgt gggaacgatc tgatcgatat
     3361 cattgaaaaa cccgaacagg aagtgtttat caacgcgggg atctacatac tcaaccccga
     3421 ggtgctcgag cgaattgtac caggaacgaa aatagatatg cctgatctga tcaagaagat
     3481 gacgggagaa aaaggcgggg tcgggtgctt cccgctgggc tcgttctgga tggacatcgg
     3541 tcgcccggac gatttcctgc gggcgcagag cgagtatcag cagcattttt cagaatcgac
     3601 cgaggtgcgt ccatgaactg gcagggaaag aaagtcgcgg tgaccggcgc tggcgggttt
     3661 atcgccagtc atctggtgga aaccctggtt cgacaaggag cacaggtgcg cgccctggta
     3721 cgctacacca gtcgcagcga caatggctgg ctcgatgacg ttcgccggaa tctgggaacc
     3781 gaattcgaga ttgtccgggg tgacattcgt gaatcgcagg gaatcatgcg cgttgtcgaa
     3841 gggtgcgact atgtctttca tctcgcggcc ctgatcgcta ttccctattc atatgattgg
     3901 cccggcagtt atattgaaac caacgtggtc ggtggattca acgtgctcga agccgccaga
     3961 gcccagcgtg tgtcgaaggt catcttcacc tcgacctcgg aagtctacgg gtcggctctc
     4021 tataccccga tggatgaaac gcacccgttg cagacccagt cgccgtatgc cgccagcaag
     4081 gctgcctctg atctcttggc cgcatcgtat gcgcgatcgt tcgagatgcc ggtggtcgtt
     4141 gtccgaccat tcaacaccta cgggccgcgt cagtcgcggc gggccgtcat tccgacgatt
     4201 atctcgcagg cacttgtggg caccgaaatc tatctcggag ataccacgcc ggtgcgcgat
     4261 ctcaatttcg tcgccgatac cgttgcggga ttcctggcct gcgccgcgaa tgcggttgcc
     4321 gatgggcgtg tctacaatct ggcgtcgggt gtcggggtca gcatcggaga ggtggtcgag
     4381 cgcatcggcc gcctgctcgg aaagccactc acgattgtgt ccgatccacg tcggcgtcgc
     4441 cccgaagcct cggaagtccg ttgcctcatc ggcgcgtcca aacgggtcga agccgaatgt
     4501 ggctggaaac ccacgttcag cctcgacgag gggttgagac aaaccgtttc ctggtttgcg
     4561 cagcacccgg agatacgctc tgccgctacg gaatccgtgc gtgagtatgt gaaatgacca
     4621 cggaacgacc cggtttcatt ccgaattgcg taccggttgt tcgtggccga gaagcagaat
     4681 acgtgcagga atgtctgaaa acgggttggg tgtcgagcgt cggtccgttc gtcgatcgtt
     4741 tcgaacgcga gttcgccgcg tatgtcggtg cccggcatgc ggttgccacc tgtaacggca
     4801 cggccgcgtt gcacctggcg cttctctgtg ctggcgtgaa acccggcgat cgcgtgctgg
     4861 taccgaccct gacgttcatt gcgacggtca atccggtgcg ctactgcggg gccgagccgc
     4921 tgctggtcga tgccgaaccg acaaccgcca atgtcgaccc gcggcagatt ctcgaaatct
     4981 gcgaacggga aagttgcgca gggcgtcgta tcgcggcgat tctcccggcc cacctgtacg
     5041 gtcaccccgc tcacctcgaa cccattcttg ccttcgcccg gcagaagaac attccggtca
     5101 tcgaagacgc atcagagtgt ctgggagggc gcctcggaga acgccacgtg ggtacctgcg
     5161 gcttgatcgg ctgtttcagt ttcaatggca acaagttgat caccagtgga ggcggtggag
     5221 gaatgctggt caccgatgac gcaacactgg cggctcgcgc caaacatttg tccgtacagg
     5281 ctcgcagtga tgaaagcgag tatctgcacg acgcggtcgg ctacaactat cggttgacca
     5341 atattcatgc ggcctttggg tgcgcgcagc tggaacagat cgaaacggtg ctggcggcga
     5401 aacgtcgtat ccatgactgg tatgagcgcg agctgcaggg agtgcccggc ctgacactgc
     5461 tgaaagagca gccgtgggcc cggtcaagtc actggatgag caacgtgctc gtcgagcctg
     5521 gggtgtttgg ggctgatcga cgtgatctgc tcagatatct cgcttcgatt ggtatccaga
     5581 cgcgtccgtt tttcaccccc atgcaccgtc tctcgatgta tcgcgcctct gaagacgcat
     5641 tcccggttgc cgagcgtctc tatcagcagg gcc
//
LOCUS       DAORHD010000113         5655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14581_length_5655_cov_3.549464, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000113 DAORHD010000000
VERSION     DAORHD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14581_length_5655_cov_3.549464"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKO06_03615"
     CDS             <1..787
                     /locus_tag="PKO06_03615"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNV68759.1"
                     /translation="VKSAVLLAALQAEGPTRIFQHVETRDHTERLLRHMASELTLDGS
                     CLVLSPNQPLRSLNLSIPGDPSSAAFFAVGAAIHPDSDVLLEGICLNPHRIRFLEVLS
                     EMGADVTCRSVVPGDWEPRGDVRVRGARLRGVTIGPEVVPGLIDEIPALAVAMAVAEG
                     ESHISGAAELRLKESDRIAVLVRELKRFGVPVLERGDGLTITGGTPLKAPVHSLPSGD
                     HRLVMALAILGTAAEGTTRIRQAECVRVSFPEFDTLLKQISRG"
     gene            921..3626
                     /gene="cmk"
                     /locus_tag="PKO06_03620"
     CDS             921..3626
                     /gene="cmk"
                     /locus_tag="PKO06_03620"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNV68760.1"
                     /translation="MNSRSEAQIIAIDGPAGAGKSTVAKKVAERLGFSYLDTGAMYRV
                     VTLKALEEKANFADVEAIRAAARSSRIRFEERPGSPMPAVFLNDRDVTEAIRTPEISR
                     NVSDVAKDAVVRDCMTKLQREIGARGHWVVDGRDIGTVVFPQATVKIFLTASIEERAQ
                     RRVKELQAKGFPADLETLKQEIARRDDIDSNRETAPLKQAEGAELVDTTSLDINQVVG
                     KIIEIVTLKGKKTRTEGRMEDVKQAAKGKKQEETMETMEQMLKAEESGEFHTVRSGAI
                     VTGTILDVTPSEIYVDINYKTDGVIEADQVPEDIRTTLKKGDKLRVYVKHIDDNRGQV
                     KLSLSRARELDAWNQIEEAFRKKTLVEGVIKGRTKGGFEVDIQGVRAFLPQSQLSHAK
                     NLKENMGKPCPMMVMEFDRRRKNVVVSERALAEEARNKRRDEVFAQCQVGDMIKGVVS
                     RIVEYGAFVDLGGGLEGLIHRNDISWSVIGNPREVLQAGQEIEAKILKMDTEKGKIGL
                     GLKQKKEDPWGTVDQRYEVGRRYEGRVKNIMDFGAFVELEEGVEGLVHISDLSWARNV
                     RHPSDIVKSGDQITVVVKEIDKTKKRISLSYKETQPHPWDNLEKKIKIGDVVQGRVNN
                     LTDFGAFIDIGEGIEGLLHISDMDWVKKINHPKEVLEKGQEVEVKILNIDSKGRRLSL
                     GLKQTQNDPWENVETTYHVGETVSGVVKNLVSYGAFIQLESGLEGLLHVSEVSWEKEI
                     KDIGEVLKVGDQVQVTVYEVDKSRQKIGLSLKRLSDDPWKGIEKRFPVNSVQPCKVKT
                     IDNSGVEVELAENIYGYIHISQIDAERVQHPSEKLSVGQEIFVKVLELDRKNRRMKLS
                     RKEAMLDSEQQELRRFQVQSNQGFSDSIGDLMRDKLEAIKEQLKG"
     gene            3727..4749
                     /gene="gap"
                     /locus_tag="PKO06_03625"
     CDS             3727..4749
                     /gene="gap"
                     /locus_tag="PKO06_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNV68761.1"
                     /translation="MTIKIGINGFGRIGRLVFRAAMSRPNVEIVGINDLFDAEYLAYM
                     LKYDTVHGTFKGTVEAKDGKLIVNGKAIRVTGEKDPANLKWNEVGADYVAECTGLFLD
                     KAGCEAHLKAGAKKVAISAPSKDDTKMFVMGVNHKSMTKDDVIVSNASCTTNCLAPIT
                     KVLQDNWGLAEGLMTTVHATTATQKTVDGPSKKDWRGGRAAAGNIIPSSTGAAKAVTK
                     VIPALKGKLTGMAFRVPTLNVSVVDLTCKLEKAPAEDTTAAYEEIKKAMKKAAEGELK
                     GILGYTEDKVVSSDFNGDARTSIFDADAGIMLNNKFVKVVAWYDNEWGYSNKLIDLIE
                     HMATLK"
     gene            4908..>5655
                     /locus_tag="PKO06_03630"
     CDS             4908..>5655
                     /locus_tag="PKO06_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNV68762.1"
                     /translation="MFRTIKDIQFKGKRTIVRVDFNVPLDKSLNITDDTRIRAAVPTL
                     KHILEQGGRLIIMSHLGRPKGGPDPKYTLKSVLKRLEELLGRKVAFAADCIGEEAEKM
                     AKSLKDGEALLLENLRFHAEEEKNEPGFAKKLAGLAEIYVSDAFGTAHRAHASTAGVA
                     DTLPAYAGFLMEKEITYLNKVTQNPERPFVAIMGGAKVSDKIKVIENLLKKVDALLIG
                     GGMAFTFIKAMGHEIGKSLCEADQIETAKKI"
BASE COUNT         1343 a         1517 c         1669 g         1126 t
ORIGIN      
        1 ggtgaaatct gcggtgttat tggccgcgct gcaggccgag ggaccgaccc ggatctttca
       61 gcacgtcgag acgcgcgatc acacggaacg attgcttcga cacatggcat cggaactgac
      121 acttgatgga agttgtctcg tactgtcgcc gaatcagccc ttgcgttctc tgaatctctc
      181 gatcccgggt gatccgagtt ctgcggcctt cttcgcggtt ggcgcggcga tccacccgga
      241 ttcagacgtt ttgctggaag gcatctgtct gaatccgcac cgcattcggt ttctcgaggt
      301 tctgtctgag atgggggccg acgtgacctg tcgttcggtg gttccaggag actgggaacc
      361 acgtggagat gtccgcgttc gtggggctcg cctgcgtggg gtgacgatcg gaccggaggt
      421 ggttcccgga ctgatagacg agattcccgc tttggccgtg gccatggcgg tggcggaggg
      481 cgagtcgcac atcagtgggg ctgcggaact gcgcttgaaa gagtcggatc ggatagcggt
      541 gctggtgcgc gagttgaaac gattcggtgt cccggtcctg gaacgtgggg atgggctcac
      601 catcacgggc ggcacgcctc tgaaagcgcc ggtacactcg ttgccatcgg gtgatcaccg
      661 gcttgtcatg gctttggcga ttctcggtac tgcagccgaa ggaacaacca gaattcgcca
      721 ggcggagtgc gtgcgggttt cttttcccga attcgatacc ttgcttaagc agatcagcag
      781 aggctaacag ctttttttct tcccctcgcg aagaagcttg caattgcatc ggaaatgttg
      841 tacattgtcg cttcaccgtt ggggtggcac ttccatctcc tgaacggttg caaccaggtc
      901 tcacactcat ggcggaacgc gtgaattcac ggagcgaagc tcaaatcatc gcaatcgatg
      961 ggcccgcagg ggcaggcaaa agtacggtcg ccaaaaaggt cgccgaacgt ctgggcttct
     1021 cctacctgga taccggtgcc atgtatcgag tggtgaccct gaaagctctt gaagaaaaag
     1081 ccaacttcgc cgatgtcgaa gcgattcgcg ctgctgcccg ttcttcgcga attcgtttcg
     1141 aagagcgccc gggctcaccg atgccagcgg tgtttctcaa tgatcgagat gttaccgaag
     1201 cgattcgcac tcctgagatc tcccgcaacg tttcagatgt cgctaaagat gcggtggtgc
     1261 gcgattgcat gaccaagtta cagcgagaga ttggtgctcg cgggcactgg gtcgttgatg
     1321 gccgcgacat cggcacggtg gtctttccgc aggcgacggt caagattttt ctgaccgcct
     1381 ccatcgagga acgagcacag cgacgcgtga aagagctgca agcgaagggc tttccggctg
     1441 atcttgagac gctcaagcaa gagattgccc gccgcgatga tattgattcg aatcgtgaga
     1501 ccgctccgct caagcaggcc gaaggagcag aattagtcga cactaccagc ctggatatca
     1561 accaggttgt tgggaaaatc attgaaattg ttacgctgaa gggtaaaaaa acccggacgg
     1621 agggacgtat ggaagatgtg aagcaagccg ctaagggaaa gaagcaggaa gaaacgatgg
     1681 aaaccatgga acagatgctg aaggctgaag aaagcggtga gttccatacg gttcggagtg
     1741 gggcaatcgt caccgggacg attctggacg tgaccccatc cgaaatctat gttgacatca
     1801 actacaagac cgatggtgtc atcgaggccg atcaggttcc cgaagatatt cggacaaccc
     1861 tgaagaaggg cgacaagctg cgggtctacg tcaagcacat tgatgacaac cgtggtcagg
     1921 tcaagctgtc gctcagtcgg gcccgtgaac tcgacgcctg gaaccagatc gaagaggcct
     1981 tccgcaagaa gaccctcgtc gagggcgtga tcaagggccg caccaagggt ggctttgaag
     2041 tcgacattca gggcgtgcgc gcgtttttgc cgcagagcca gttgtcgcat gccaagaatc
     2101 tcaaagaaaa catgggcaaa ccctgcccga tgatggtcat ggaattcgac cgccgccgca
     2161 agaacgtcgt tgtctctgaa cgtgcgctgg ccgaagaagc ccgtaacaag cgccgcgacg
     2221 aagtgttcgc gcagtgccag gtcggcgaca tgatcaaggg cgtggtttca cgcatcgtgg
     2281 aatacggcgc attcgttgat ctcggcggtg gtcttgaagg cctcattcac cgcaatgaca
     2341 tcagctggtc agtgattggc aatcctcgtg aagtgcttca ggctggccag gagatcgaag
     2401 cgaagatcct gaaaatggat accgaaaagg gcaagatcgg tctgggcctc aagcagaaga
     2461 aagaagaccc ctggggcacg gttgatcagc gttacgaagt gggccgccgt tacgaaggcc
     2521 gcgtgaagaa catcatggat ttcggcgctt tcgttgagct cgaagaaggc gttgaaggtc
     2581 ttgttcatat ttccgatctc tcctgggccc gcaacgtgcg ccatcccagc gatatcgtga
     2641 agtctggcga tcagatcacc gtcgtggtga aagaaatcga caagaccaag aagcgcatct
     2701 ctctttctta caaagaaact cagccccatc cctgggacaa cctcgagaag aagatcaaga
     2761 tcggcgatgt cgtgcagggc agagtgaaca acctgaccga cttcggcgcg ttcatcgata
     2821 tcggtgaagg catcgaaggt ctgctgcaca tctccgacat ggattgggta aagaaaatca
     2881 accacccgaa agaagtcctc gagaagggcc aggaagtcga ggtcaagatt ctcaacatcg
     2941 actccaaggg tcgccgcctg tcgttgggcc tcaagcagac tcagaacgac ccgtgggaaa
     3001 acgtcgagac cacgtatcac gtgggtgaga cggtcagcgg tgtcgtgaag aacctcgtca
     3061 gctatggtgc gttcattcag ctcgagagcg gccttgaagg cctgctgcat gtttccgaag
     3121 tttcctggga aaaagaaatc aaggacatcg gcgaagtgct caaggttggc gatcaggtgc
     3181 aggtgaccgt gtatgaagtc gacaagagcc gccagaagat cggcctctcg ctcaagcgtc
     3241 tgagtgacga tccctggaag ggcatcgaga aacgcttccc ggtgaacagc gttcagccct
     3301 gcaaggtcaa gaccattgac aatagcggtg tcgaagttga actggccgag aacatctacg
     3361 gatacattca catctctcag atcgatgccg agcgggtgca gcaccccagt gaaaagctca
     3421 gcgtcggcca ggagatcttc gtgaaggtgc tcgaactcga ccgcaagaac cgccgcatga
     3481 aactctctcg caaggaagcg atgcttgact ccgaacagca ggaactccgc cgattccagg
     3541 tgcagtccaa ccagggcttc tccgattcca tcggcgacct gatgcgcgac aagctcgaag
     3601 ccatcaagga acagctcaag gggtaagtcc ccacgtatgg tgatgaagct tacccgccct
     3661 ggatcctgat ggacccgggc gggttttttc tcgaccgcat aataaacatc tcaggaggct
     3721 gtacgtatga ccatcaagat cggaatcaac gggtttggac ggatcggccg gttggtgttt
     3781 cgggctgcca tgagccgccc caatgtcgaa atcgtcggca tcaacgacct gttcgatgct
     3841 gaatatctcg cctatatgct caagtacgac accgtgcacg gcacgttcaa gggcaccgtg
     3901 gaagcgaaag acggcaaact gatcgtgaat ggcaaagcca ttcgcgtcac cggtgaaaaa
     3961 gacccggcga acctgaagtg gaatgaagtc ggcgctgact atgtcgccga atgtactggt
     4021 ttgtttcttg acaaagctgg ttgcgaagct catctgaagg ctggcgcgaa gaaggtcgcg
     4081 atctccgctc cctcgaagga cgacaccaag atgttcgtca tgggcgtcaa ccacaagagc
     4141 atgaccaaag acgacgtcat cgtgtcgaat gcctcctgca ccaccaactg tctggccccg
     4201 atcaccaagg ttctgcagga caactggggt ctggccgaag gtctgatgac caccgtgcat
     4261 gcgaccaccg ccacgcagaa gaccgttgac ggcccgtcga agaaagactg gcgcggtggc
     4321 cgcgcagctg cgggtaacat cattccctcc tcgacgggcg cggccaaggc cgtgaccaag
     4381 gtcattcctg cgctcaaggg caagctgacc ggtatggcgt tccgcgttcc gaccctcaac
     4441 gtgtcggtcg ttgacctgac ctgcaagctc gaaaaagcgc cggccgaaga taccaccgcc
     4501 gcctacgaag aaatcaagaa agccatgaag aaagccgctg agggcgaact gaagggcatt
     4561 cttggctaca ccgaagacaa ggtcgtcagc agcgacttca acggggatgc ccgcacctcg
     4621 atcttcgatg ccgatgccgg gatcatgctc aacaacaagt ttgtcaaggt tgtcgcctgg
     4681 tacgataacg agtggggcta ttccaacaag ctcatcgatc tcatcgaaca catggcgacc
     4741 ttgaagtaag tgccgttctc tcgcagcgat cgatgttttc gcctgcggag gccggaccaa
     4801 ccgtgaaatg aagcggggcc ccgcgttcgg ggctccgctt gtttttgtcc ctgaaatggt
     4861 ttattgatgt agcacacctt cagaaacagg aggaaatgag gaatcctatg ttccgaacga
     4921 tcaaagacat tcagttcaag ggaaagcgca ccatcgtccg tgtcgatttc aatgttccgc
     4981 tcgacaagag cctgaacatc accgacgata ctcgcatccg tgccgcagtt ccgacgctca
     5041 agcacatcct cgagcagggc ggccggctga tcatcatgtc gcatctgggc cgtcccaagg
     5101 gcgggcctga cccgaaatac acgctcaaat ccgtgctcaa gcgcctcgaa gagctgctgg
     5161 gccgcaaagt tgcttttgct gcggattgta tcggtgaaga agccgagaag atggccaaga
     5221 gcctgaaaga cggtgaagct ctgctgctcg agaaccttcg gttccacgcc gaagaagaga
     5281 agaacgagcc cgggttcgcc aagaagctgg ccggtcttgc cgagatctac gtcagtgacg
     5341 cgttcggcac ggcgcatcgc gcgcacgctt cgactgccgg tgtggctgat actctgcccg
     5401 cctatgccgg tttcctgatg gaaaaggaaa tcacctacct gaacaaggtg acgcagaatc
     5461 ccgaacgccc ctttgtggca atcatgggcg gcgccaaggt ttctgacaag atcaaggtta
     5521 tcgagaacct gctcaagaaa gtcgacgcgc tgctcattgg cggtggcatg gcctttacgt
     5581 tcatcaaggc catgggtcac gagattggca agtcgctctg cgaagccgat cagatcgaga
     5641 cagcaaagaa gatca
//
LOCUS       DAORHD010000114         5654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14584_length_5654_cov_3.545812, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000114 DAORHD010000000
VERSION     DAORHD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14584_length_5654_cov_3.545812"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /gene="rfbF"
                     /locus_tag="PKO06_03635"
     CDS             complement(<1..724)
                     /gene="rfbF"
                     /locus_tag="PKO06_03635"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HNV68763.1"
                     /translation="MKAVILAGGLGTRLSEETVVKPKPMVEIGGKPILWHIMKIYSTY
                     GINDFVVCLGYKGYMIKEYFANYFLHMSDVTFDMKCNEMKVHHNKAEPWTVTLIDTGE
                     STATGGRLRKVRSFLDEETFCFTYGDGVADVNISSLIEFHRRSGLKATLTAVQPPGRY
                     GAVNVERELVESFQEKPKGDGGWINGGFFVLEPDAIEYVKGDQTSWEGDSLVRLATDH
                     QLGAFKHDGFWRPMDTLRDKMYL"
     gene            complement(969..1661)
                     /locus_tag="PKO06_03640"
     CDS             complement(969..1661)
                     /locus_tag="PKO06_03640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV68764.1"
                     /translation="MNNIYRDGTYLESNPDWHVKDSPWKAKQIADLLARNRFVPETVC
                     EVGCGAGEILACLSKIYGDKVTFHGYEISPQAFEICRRKEQPNVCFHFADLLVTEKVF
                     DVVMAIDVFEHVENYIGFLRDLKPKGTYKLFHIPLDLSVFAVLRCSPILKARHSVGHL
                     HYFTKETALETLRDTGYQIVDWCYTRDSLELPQLGWPATMMKLPRRLFFALHQDLAAR
                     ILGGFSLLVLTK"
     gene            1935..3596
                     /locus_tag="PKO06_03645"
     CDS             1935..3596
                     /locus_tag="PKO06_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68765.1"
                     /translation="MKKPVVPFVPPLRSGTLKGLDSLAGLPPENSKESAADRVVGRLT
                     PAKPFPAVTPTDHSSSREWRIQKHFLSLYDKLGKPFARFLSDLLDILLSGSIQNRLVS
                     ELIEARVQEMRREKAEEARESGAMHRKVTPSRPVEVTIGGEPRQIPRHFLKRKSLIAG
                     MLLNTKEFSGFQKLWNALEQVVRTRETEQLLSGLDRPARRKVLEEIVGRLKLEICHED
                     LVAAWALLEADQFEDLEWAYTETRMLSIVRPAFYEDLDTAYREAKKLLKLLISEFEEV
                     FPPEVAKKDAILDYLREILEVYTHHHFVLKYFNQSRPSLTRKEWESTRQDLLVKIESL
                     KAEALRLRDENRSLLVERDRAVAQMRTAAQQAEESRQQLQKLDPAEVARQRRAMESEM
                     NQLKTELTEAQEEENHLRLQMRKLDAENQSLLQQLRDLKAIPDESAKSVAGMLEGKRV
                     VIFGGVGRDHYLPVLKEAGVKESDFVWYEGYRTISQNRTAEIMGRCDFIVVITSYAGH
                     LHTWQTRSSRTEGQVLEYIHNSGTGTLRQKIIERFGPGDRSDSRS"
     gene            3651..4232
                     /locus_tag="PKO06_03650"
     CDS             3651..4232
                     /locus_tag="PKO06_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV68766.1"
                     /translation="MTPEELWREALRGHQPSWEALYRLLGGRLYQFFLKNTGKPELAM
                     DKTQEVFERLWKHRESFIDGSLNTWVFRIARNLLIDLWRRKPRAEILAEDPPEVVDPA
                     IGVEESVLTKLHREDMLRHIDDSLNELSEDDRLILGVVYLGGLSLPELASVMEIPLGT
                     AKTRCRQARLRLDRLLQERLKGQTPTLEAKETA"
     gene            4229..5587
                     /locus_tag="PKO06_03655"
     CDS             4229..5587
                     /locus_tag="PKO06_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68767.1"
                     /translation="MNCQEFQEKLLEPTTLAAFDQNPEASAHAAACAECRRLLEWNRK
                     VAEGFSAMSLREPPAILRDRILRIPELVTREDASAKPVAETETAWFPGWRWLSGMGLS
                     FAGAFLLYVLVVRPFDMGTAVREDSRPTTKGMQVSAVPPASVSEAVRPSPEPVIPALQ
                     PTSAEAKPPDTGAAVPSSPAIAIEVAKVPTVSTAKTTASSLPVSAPASAVAIGRVAVD
                     EASTAMQVAAAPMAPEPKNRMAVTENQEPPATEMAFAPPPPKVVGTPLNTVVGEPLVP
                     TADAPSASVSTAAGAGNTSPGASKSEEPIVLAQTENKGSQFGLADKRPSFTEGEILLR
                     EAVPADAEEAKAGSLDDQPLLDTSATSDQFAREFRASKKETTAMPTQQALPDLETRKE
                     RLLAIVQKHSDKVIEGPIDLNQWILAGWITVKERISLAAPQGRTWVTQHEDGQWKIEL
                     EP"
BASE COUNT         1316 a         1527 c         1573 g         1238 t
ORIGIN      
        1 cgagatacat cttgtcgcgc agggtatcca tgggccgcca gaacccatca tgtttgaaag
       61 cgcccagctg gtgatctgtg gccagccgaa cgagtgaatc accttcccag ctcgtttgat
      121 cgcctttcac atactcgatg gcgtcaggtt cgagtacgaa gaagccgccg ttgatccagc
      181 ctccgtcacc cttgggtttt tcctgaaagc tctcaaccaa ctctcgttcg acgttgacgg
      241 ctccgtaccg accagggggt tggacggcgg taagcgtcgc tttcaagcca gaccgacggt
      301 gaaactcgat gagtgaactg atgtttacat ctgcaacacc gtctccgtag gtgaaacaga
      361 atgtttcctc gtcgagaaaa gagcgaactt tcctgaggcg tccaccagtt gctgtcgatt
      421 ccccggtgtc gatcagtgtg accgtccagg gttccgcctt gttgtgatgc actttcatct
      481 cgttgcattt catgtcgaag gtgacgtcgg acatgtgcag aaagtagttg gcgaaatact
      541 ccttgatcat gtaacccttg tacccgaggc acacgacaaa atcgtttatc ccgtaggtcg
      601 aatagatctt catgatgtgc cacagaatcg gtttgccacc gatctcgacc attggcttgg
      661 gtttgacgac cgtctcttca ctgagtcttg tgcctaatcc accggctagg ataacggctt
      721 tcatgagcat tcctcgattg tctgcaaatg tagtgatctc tgttagattc tgaaccgata
      781 cggcggcgtc attgaaacga caacagaatc ggtgtgagcc tgcctcatct cccgtgttgt
      841 tggaggcatt cattcaggtt cgcgaatggc gagagaatgc taccaccaga gtgcgacgaa
      901 tgtctacagt atgatcagat gaggttgtgc gaggtgggaa cggaaacgtc gggcgcccag
      961 cctgcttgtc acttggtcag gacaagcaga gaaaatccac cgaggatacg cgctgctaga
     1021 tcttgatgga gagcaaagaa cagacgtctt ggcagtttca tcatcgtcgc cggccagccg
     1081 agttgcggga gttccagcga gtctctggtg taacaccaat cgacaatctg gtatccagtg
     1141 tctcgaagtg tttcgagtgc tgtctccttc gtgaagtaat ggagatgacc gacggagtgg
     1201 cgtgccttca gaataggtga acagcgcaat acggcgaaca cagacaaatc gagaggaatg
     1261 tgaaaaagct tgtatgttcc ttttggcttc aggtcgcgca agaatccgat gtagttttct
     1321 acatgttcga atacatcgat cgccatcacc acatcgaaga ccttctcagt gacgagcagg
     1381 tccgcgaaat gaaaacagac gtttggctgt tccttcctgc gacagatctc gaaagcctgg
     1441 ggtgaaatct catagccatg aaaggttacc ttatctccgt agattttcga caaacatgcg
     1501 agaatttcgc ctgccccgca accgacttcg caaacagtct ctgggacaaa ccgatttctc
     1561 gcgagcaaat ctgcaatttg cttggctttc cacggtgagt ctttcacatg ccagtcagga
     1621 ttactttcga gatacgtccc gtctctgtag atgttgttca ccggcacgcc tttacctaaa
     1681 tgatttcata cggaacatgc agcttggatg gcctcgatga gatgatcgga ggtagtatat
     1741 accaaatgct tcagcacagc ccgaacaggt ttttccgtca cgacggcgag tgagctgata
     1801 gttcgcctgc tgtcctgatt tccgcaccgg ggtgcagggt ggcttgttcg taccccatac
     1861 aaacagctga cttctagtat tgccttgccg tctgaatttg cgtagactgc gggaatctct
     1921 ggttcggagc acgtatgaaa aagcctgttg tcccatttgt gccaccgttg cggtctggaa
     1981 cactcaaagg gctggactcg ctggctggtt taccacctga aaactcaaag gaatcggctg
     2041 ccgatcgggt tgtcgggcga ctcactccgg ccaagccttt tccggcggtg actccaacag
     2101 atcactcctc cagtcgcgaa tggcgtattc agaaacactt tttgtcgctg tatgacaaac
     2161 tcggaaagcc gtttgcccgc tttttgagcg atctgctcga catcctgctg agcggctcga
     2221 tccagaatcg gctggtgagc gaactgattg aagcgagagt ccaggaaatg cgccgcgaaa
     2281 aagcagaaga ggcgcgcgaa agcggggcga tgcatcgaaa agtgactccc agtcgtcctg
     2341 ttgaagtgac cattggtggc gagccacggc agattccccg tcactttctc aagcgcaaga
     2401 gcctgattgc cggaatgctg ctcaatacaa aagaattttc cggttttcag aaactctgga
     2461 atgccctgga acaggttgtg cgaacgcgag aaaccgagca gctgttgtct gggctcgatc
     2521 gccccgcacg gcggaaagtt cttgaagaga tcgttgggcg attgaaactc gaaatctgcc
     2581 atgaagacct ggttgccgca tgggctctcc ttgaagccga tcagttcgaa gatctcgagt
     2641 gggcttacac ggaaacccgt atgctgagca ttgtgcgacc ggcattttac gaagaccttg
     2701 acacagccta tcgcgaagcg aaaaaactgc tcaaactgct gatttccgag tttgaagagg
     2761 tctttccacc tgaggttgcc aagaaagacg cgattctcga ctacctgcgg gagatcctgg
     2821 aagtctatac ccatcaccat tttgttctga aatacttcaa tcagagccgc ccatctctca
     2881 cccgcaagga gtgggaatct acccgccagg acctactcgt gaagatagaa tcactgaagg
     2941 ctgaagcact cagattgcga gatgagaacc ggtctctcct ggtcgagcgt gaccgggccg
     3001 tggcacagat gcggacagcg gctcaacagg cggaagagag tcgccagcag ctccagaagc
     3061 ttgatccggc cgaagtcgcc cgtcagcgtc gtgccatgga atctgagatg aaccagctca
     3121 aaaccgagct gacagaggcc caggaggaag aaaaccacct gcggctgcag atgcgcaaac
     3181 tcgatgccga gaatcagagc cttctgcagc aactgcgcga tctgaaggcc atcccggatg
     3241 aatcggccaa atcggttgcc ggcatgctcg aaggcaagcg ggtcgtgatt ttcggtggcg
     3301 ttggccgcga tcactatctg cccgtgctga aagaggcagg ggtcaaggaa agcgacttcg
     3361 tgtggtacga aggctatcgc acgatcagcc agaatcgtac ggctgagatc atgggccgat
     3421 gtgacttcat tgtggttatc accagctatg ctggtcacct gcatacgtgg cagacccgtt
     3481 cctctcgaac agaggggcag gttctcgagt acattcataa cagtggcacg ggaacactgc
     3541 gccagaagat catcgagcgc tttggtccag gcgatcgttc cgatagccgt tcttgacccg
     3601 tcgctacgtt cctaaatcca atggctatga ctcacgttga aaaaagtgac atgacccccg
     3661 aagaactctg gcgtgaagcg ctgagaggtc accagccgtc ctgggaagcc ctttaccggc
     3721 tgctgggggg gcgattgtat cagttctttc tcaaaaacac cggaaagccc gaactggcaa
     3781 tggacaagac acaggaagtc tttgaacgtc tctggaagca tcgggagtcg ttcatcgatg
     3841 gaagcctgaa tacctgggtc tttcgtattg ctcgcaatct cctgatcgat ctctggcgtc
     3901 ggaaaccgcg tgccgagatt ctcgccgaag acccgcccga ggtggttgac ccggcgatcg
     3961 gggtcgaaga gagcgtgctg acgaaactgc atcgcgaaga tatgctgaga catattgatg
     4021 actcgctcaa cgagctgtct gaggatgatc gtctgatact cggagtagtc tatctcggtg
     4081 ggctttccct tccggaactg gcctcggtca tggagatccc gcttgggacc gccaagacca
     4141 gatgccgcca ggcccggtta cgactcgatc gattgttaca ggaacgtctg aaggggcaaa
     4201 ctcccactct ggaggcgaag gaaaccgcat gaactgccag gaattccagg aaaaacttct
     4261 cgaaccgacg actcttgccg cgttcgacca gaacccggaa gcgtcggcgc atgcggctgc
     4321 gtgcgctgaa tgccgccgtc tgctggaatg gaaccgcaag gtggctgagg ggttcagcgc
     4381 gatgtccctg cgtgagcctc cggctatcct gcgagatcgc attctccgta ttccagaact
     4441 ggtcacccgt gaggatgcat cggcaaagcc tgtcgctgaa acagaaaccg catggttccc
     4501 gggttggcgc tggctttccg gcatgggatt gagctttgcg ggcgcttttc tgttatatgt
     4561 gctcgtggtg cgaccattcg acatgggaac tgcggtgcgt gaagactcgc gcccaacgac
     4621 gaaaggcatg caggtttccg ctgttccgcc tgcatctgta tctgaggctg tgcgcccatc
     4681 gcccgaacca gtgattccag cgttgcaacc gacatccgca gaagcaaaac cacctgacac
     4741 gggtgcagcg gtcccttcgt ctccagcgat tgccatcgag gtggcgaaag tgccgacggt
     4801 gtcaactgct aaaacaactg catcttcgct gccggtctca gcacctgcga gcgctgtggc
     4861 gattggccga gtagccgtcg atgaggcctc aactgccatg caggttgctg ccgcgccgat
     4921 ggcccccgag ccgaagaacc ggatggcggt gaccgaaaat caagagcctc cggcaacgga
     4981 gatggcgttt gcaccgcctc cgccaaaagt cgtaggaacc ccgttgaaca ccgtggttgg
     5041 tgaaccgctg gttccgactg ctgatgcgcc atctgcctcg gtgagcacag ctgcgggtgc
     5101 gggaaacacc tcgcccggag ccagtaaatc agaagagccg attgtcttgg cccagacaga
     5161 gaacaaaggc tcacaattcg gtctggcaga caagcgacct tcatttaccg aaggcgagat
     5221 cctgctgcgt gaggccgttc cggcagatgc cgaagaagcc aaggccggtt ctctcgatga
     5281 tcagcccttg cttgacacgt cggcaaccag cgaccagttc gcgcgcgagt tccgcgcctc
     5341 aaagaaggaa accactgcga tgccgactca acaggcgttg cctgatctcg agacgcgaaa
     5401 agaacgatta ctcgccattg ttcagaaaca ctcggacaag gttatcgaag gtccgattga
     5461 tctgaaccaa tggatactgg ccggatggat cacggtgaag gagcgcatca gcctcgcagc
     5521 accccaggga cgaacctggg tcacgcagca cgaggacggc cagtggaaaa ttgaactcga
     5581 accctgagcc cccttaacac ttttttacat cgcggactgg ttcccggcaa agtaacgata
     5641 gatccgtgtt tcag
//
LOCUS       DAORHD010000115         5634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14656_length_5634_cov_3.619107, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000115 DAORHD010000000
VERSION     DAORHD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14656_length_5634_cov_3.619107"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..406
                     /locus_tag="PKO06_03660"
     CDS             155..406
                     /locus_tag="PKO06_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68768.1"
                     /translation="MVVHVSTIRGLDEALAELERAIESITDAETKKQLQKKYQRLSEA
                     RRTMSGTPCPPPMTSKILFMVGLTALVLVLLVHYSHRRS"
     gene            410..1042
                     /locus_tag="PKO06_03665"
     CDS             410..1042
                     /locus_tag="PKO06_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68769.1"
                     /translation="MRHPLLLVLIVLWFVGTFGVLQADENASATTTTNKPDNRPDKVE
                     RDWGVGYFRLNGYHDFVEPRISFIKRKPHYSEVIEYSWRPMEVVDMPKHERIDRIRCL
                     INRYYFTGRERRFFYGAGLGGNIVLFNDDLKDWAKSRGLSLEDGVHGAGRVFVGYKIR
                     EVRFMKKLYPVVARIDAHISPDYKFGGILGRAGDRLELSEIKFGLNFSIE"
     gene            1073..2008
                     /locus_tag="PKO06_03670"
     CDS             1073..2008
                     /locus_tag="PKO06_03670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68770.1"
                     /translation="MRKYVFGPVPSRRLGRSLGIDLIPPKTCTFECRYCQLSSTTTLL
                     TQPQSFYPIEDILAELHEILNDIDRPDWITLSGAGEPTLHADLGTIIRRIKELHVAPV
                     CVITNGTLLHLPSVQKALLPADRVIPTLCTVFPETFTRIHRPAPGIDLCNTLQGLPRF
                     AAQFQGVLEVEIFVVPGINDTPQEVAGLSRFLSTLPRLAAVYLNTAVRPPHDASLRPA
                     TREELQQFKNRLALMVPVTTALDHQPVPKRLTRKQQATPEDILALLLRHPCTIEQLCM
                     VLDQTPSELQGVLDELQRQGKITQWPDLSWGVAES"
     gene            2270..3064
                     /locus_tag="PKO06_03675"
     CDS             2270..3064
                     /locus_tag="PKO06_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68771.1"
                     /translation="MMKPHVSRAERRALIDGFLAHDPNALDRFYEQVLHHRLEALYGC
                     RLLFPPAVPIRFFDQQERPGLWHCLGPCLLLLRGHLLRRWICRQSVSEADAEAWILRL
                     RTGVHQRDYEVLPRDFRTRLERLGGIAYPGIRVWSHPHLYRAWTVDDSGQACTADILL
                     LLTLADWWDAHAGEIPLLPENDVWFTDSIRMGSELEVEADHETLRAALNDLREEFRQA
                     AVREGYQRTGWEEAQSAWDQSEEDDDTEEVEEGYDRENDGSDEDED"
     gene            complement(3152..4303)
                     /gene="ccsB"
                     /locus_tag="PKO06_03680"
     CDS             complement(3152..4303)
                     /gene="ccsB"
                     /locus_tag="PKO06_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HNV68772.1"
                     /translation="MSPESIVFALSMASYIFAFVIFTFSQALQKTGLYRFGRFALLIG
                     GILGATGIILRWSASGHPPLSNMYESLVTLATMTAWIAWLFSLRHPLPLVEGGAAVLS
                     VLMIGIASVFPRDIKPLVPALQSGWLYLHVSIAFVGEACFALAFAMSFLFCFRRLITG
                     RQSQRRSERKADGVHTAWTAERLLCAGIVLGLPLILTLGLAKATVVSVPVVPVTMAVP
                     PESTAVASAALATNNLPATTWEFKPIMLWVTVPVALLTLLLFLSFFLLRGAAGRAVDQ
                     WLPEEDRLDEFTYRAIALGFPLFTVGAIIFGMVWAQKAWGRYWGWDPKETWALITFLV
                     YSLYLHLRLTKGWRGLGAAVLSVVGFLVTMFTLFGVNLLLSGLHSYASF"
     gene            complement(4300..>5634)
                     /locus_tag="PKO06_03685"
     CDS             complement(4300..>5634)
                     /locus_tag="PKO06_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HNV68773.1"
                     /translation="FGLLAVLALSTLLCTLTRLKLTRQRLFQRLDYLSPREILSLPVR
                     VELDPTKVLLKRDTGWQSRTFDDGLVTHFRPVGRLALIGGALIHVGLLILLAGGLWSR
                     TVYVETAIHGMEGERVALPTIEALQAGAQADRYRRQVRAAQSFNASDARMVALAAEID
                     RLDSIYKQGVMQPAFKISFDRLWVDVHEVASASDLPMTRNWNTQGTVQQADQVVASAV
                     IRVNEPFSYGGLSFYQADWKKVYREVRLKGVPIGEEGIRYLGSQPIELIASLGAPISL
                     ENSSYTIVVLDFWPDFRFMGAEPVSVSDELRNPSARLAIYDGEGKLAGRAWAFGEELE
                     ELSGHLSNLPVRFLVTGAAPRFESVLQVAADPAIPVVWLGSLIMTLGMFLTLYVSYRE
                     EWMVEQTNGKVLLAVNGNRAPFLLLESLRSLLQRVAPEATLPEDLLRERSDT"
BASE COUNT         1287 a         1732 c         1513 g         1102 t
ORIGIN      
        1 cctgttggtc tttgcgcaag tctgccagcg tttgaatgat caggtccggc ttcgcgaggt
       61 aatcgccaag ctgcagggca tgaatttgtc agagtcacaa ctcgagcagc tccgtgagct
      121 gcagaatact cactgaccgg gaggaggaga cgacgtggta gtgcatgttt cgaccatacg
      181 cggcctggac gaggctctgg ccgaactcga acgagccatc gaatcgatca cggatgctga
      241 gacgaaaaag cagttgcaga aaaagtacca gcgtctttcc gaggctcgcc gcaccatgag
      301 cggaacccct tgtccgccac ccatgaccag caagattctc ttcatggtcg gactgacggc
      361 gttagtcctc gtgcttctgg tgcactactc tcaccgccgt tcctgatcga tgcgacatcc
      421 tcttctcctg gttctgatcg ttctgtggtt cgtcggcacc ttcggcgtac ttcaggctga
      481 cgagaacgcc tccgccacca ccacaacaaa caagcctgac aatcgccctg acaaggttga
      541 gcgcgactgg ggcgtcgggt attttcgact caacggctat catgactttg tcgagccgcg
      601 catctccttc atcaaacgca agccccacta ctccgaggtg atcgagtatt cgtggcggcc
      661 catggaagtg gtcgacatgc ccaaacacga gcgcatcgac cgcattcgct gtctcatcaa
      721 ccggtactat tttaccggtc gcgagcgccg ttttttctat ggcgccggac tgggaggcaa
      781 catcgtcctc tttaacgacg atctcaagga ctgggccaaa agccgcggtc tctctctcga
      841 agacggagta catggagccg ggcgcgtctt tgtcgggtac aaaattcgag aagtccgatt
      901 catgaagaaa ctctacccag tggtagcgcg catcgacgcc catatctcac cagactacaa
      961 gtttggcgga atactcgggc gggcaggcga tcggctcgaa ctgagcgaaa tcaagttcgg
     1021 actgaatttc agtatcgagt aacctcgaga aaaggaactg caaggggcaa ccatgaggaa
     1081 gtatgttttc ggacctgtcc cttcacgaag gctcggacga tcgctgggca tcgatctgat
     1141 tccccccaag acctgtacct tcgaatgccg ctactgccaa ctttcatcga ccaccactct
     1201 gctgacgcag ccgcagtcgt tctacccgat cgaggacatt ctcgcggaac tgcacgagat
     1261 tctcaacgac atcgaccgcc cggactggat caccctttcc ggggcaggtg aacccacctt
     1321 gcatgcagat ctcggcacga tcattcgtcg catcaaggaa ctgcacgtcg ccccggtctg
     1381 tgtcatcacc aacggcacat tgctgcatct tccttcggtt caaaaagcct tgctgccagc
     1441 cgatcgcgtg attccaactc tctgcaccgt gtttcccgag acctttacgc gtattcaccg
     1501 accagctccc ggcattgatc tctgcaacac tcttcaagga cttccccgtt ttgccgcgca
     1561 gtttcagggt gtgcttgaag tcgagatctt cgtcgttccc ggcatcaatg atacgcctca
     1621 ggaagtcgcg ggcctcagtc gctttctttc gacgctgccg cgactcgctg ccgtgtatct
     1681 gaatacggcg gttcgcccgc cacacgacgc atcgttacgc ccagccaccc gcgaagagct
     1741 gcagcagttc aaaaatcgat tggccctgat ggtgccggtg accaccgccc tcgatcatca
     1801 accggtaccg aaacggctga cccgcaagca acaggcaact ccggaagaca ttctcgcctt
     1861 actgctcaga cacccctgca cgatcgagca gctctgcatg gtcctcgatc aaaccccatc
     1921 tgaactgcag ggcgtgcttg acgaattgca gcgtcagggc aagatcacgc agtggcccga
     1981 tctttcctgg ggtgtcgccg aatcctgacc ctttcctcac tccttcactc acactccctg
     2041 tatgccatca aactcagaca tcatcattcc ttcgcaccga ggcgcgtatg atcgcctctg
     2101 gtctgagttt cgtcacgtcg ttctttcggt atcattgaag gctccctccc gcttcacgcg
     2161 caaattgtgc tcggttccaa cccgtactct gagttttctg gaagatctga gcggtttctg
     2221 tcgtcagcca cgcacacttt ttctgcaatc actgcttcag gcatatctcg tgatgaaacc
     2281 tcacgtctca cgcgcagagc gtcgagcgct tatcgatggc tttctcgcgc acgacccgaa
     2341 tgcgctcgat cggttttacg agcaggtcct gcaccaccgg ctggaagccc tgtatggttg
     2401 tcgcctgctc tttccacccg ctgtgcccat acgatttttt gaccagcagg aacgtccggg
     2461 gctctggcac tgtctcggtc cctgtctcct gctgctgcgc gggcatttgc tgcgccgctg
     2521 gatctgccgt cagtccgtgt cggaagcgga cgccgaggcc tggattctcc gtctgcggac
     2581 cggggttcat cagcgtgact acgaagtgct tccccgggat ttccgtactc gcctcgagcg
     2641 actgggaggt atcgcatacc cgggaatacg cgtctggtcg cacccacatc tctatcgagc
     2701 ctggactgtc gatgacagcg gtcaggcatg cacggcagac attctgttgt tactgacgct
     2761 cgccgactgg tgggacgctc acgcaggaga gattcccctg ttgccggaaa acgacgtctg
     2821 gttcacggat tccatcagga tggggagtga actggaagtc gaagcagatc acgaaacgct
     2881 tcgcgccgct ctcaacgacc tgcgagagga gtttcgccag gcggcggtac gcgaaggtta
     2941 tcaacgtact ggctgggaag aagctcaatc ggcctgggat caaagcgaag aagatgatga
     3001 taccgaagag gtcgaagaag gatatgaccg cgagaacgat ggatcagacg aagacgagga
     3061 ctgacctttc cgattctctc aaaccggttc acactatcac tttccgccgg ggcccgatca
     3121 gatgcccgca ttggccgctc cggcgtcaat atcagaacga agcgtaggag tgaagcccgg
     3181 acagcagaag gttgacgcca aacagagtga acatggtcac cagaaaaccg acgaccgaaa
     3241 gcaccgcggc accgaggcca cgccaccctt tcgtcaagcg caggtgcaga tagagcgaat
     3301 agacgagaaa ggtaatgaga gcccaggttt ccttggggtc ccagccccag taacgccccc
     3361 aggctttctg cgcccacacc ataccgaaga tgatcgcacc gaccgtaaac agcggaaatc
     3421 cgagcgcgat cgcccgatac gtaaactcgt cgagacggtc ttcctcggga agccactgat
     3481 caacggcccg ccccgcggcc ccgcgcaaca gaaagaacga aagaaacaac aacagcgtca
     3541 gcagtgcgac cggaactgtc acccagagca tgatcggctt gaactcccag gtggtcgccg
     3601 gaaggttgtt cgtcgccagc gcggctgaag cgacagcagt gctctcgggt ggcacagcca
     3661 tggtcaccgg aacgaccggc acggatacca cggtcgcttt cgccagacca agcgtgagaa
     3721 tcagcggcaa acccagcacg atgccggcac acagtagccg ttccgcggtc caggcggtat
     3781 ggacgccatc agcttttcgt tcgcttcgac gctggctttg ccggccggtg atcaagcgac
     3841 gaaaacagaa gaggaaactc atggcaaacg cgagcgcaaa gcaggcttcc cccacgaaag
     3901 cgatcgacac atgaaggtag agccatccgg attgcagggc cggaaccaac ggtttgatat
     3961 cgcggggaaa caccgaagca atgccgatca tcaacaccga cagcaccgcg gcaccaccct
     4021 ctaccagagg taacgggtgc cgtagggaaa acagccacgc aatccaggcg gtcatcgtgg
     4081 ccagcgtaac cagcgactcg tacatgttcg aaaggggagg atgcccgctt gccgaccatc
     4141 gcagaataat tcctgtcgcg cctaatatgc ctccgataag caacgcgaat cgtccgaacc
     4201 ggtacaaacc cgttttctgc agagcttgag aaaacgtaaa gatgacaaaa gcgaaaatgt
     4261 acgatgccat gctgagcgca aagacaatcg attccggact catgtgtcag acctttcgcg
     4321 cagcagatct tccgggagag tggcttcggg agcaacccgc tgcagcagtg aacgcagact
     4381 ttccaacagc agaaatggag cgcggtttcc attcacggcc agcaacactt tgccgttcgt
     4441 ctgttccacc atccattctt cacgatacga aacatacagg gtcagaaaca tcccgagcgt
     4501 catgatcagg cttcccagcc agaccacagg aatcgccggg tcggcagcga cctgcagcac
     4561 gctttcgaat cgcggcgcgg cacccgtcac cagaaagcgt accggaagat tcgacagatg
     4621 cccggacagt tcttcgagct cttcaccgaa ggcccaggcg cggccggcca gttttccctc
     4681 gccatcatag atcgccaggc gagccgacgg attccgcagt tcatctgaga ccgagaccgg
     4741 ctcagcaccc ataaagcgaa agtcaggcca gaagtcgaga acgacgatcg tgtaagaact
     4801 gttctcgagg gaaatcggag cccccaggga ggcgatcagc tcgatgggct gagatccgag
     4861 ataccgtatt ccttcttctc cgatgggaac gcccttcagg cgcacttccc gatagacctt
     4921 tttccagtct gcctgataaa acgaaagtcc accgtaggag aacggttcat tcacgcgtat
     4981 gactgcgctg gcgacaacct gatcggcctg ttgcaccgtc ccctgcgtgt tccagtttcg
     5041 ggtcatcggc aggtcgctcg ccgaagccac ctcatgcaca tcaacccaca aacggtcgaa
     5101 agagatcttg aacgcgggct gcatgacccc ttgtttatag atcgaatcaa gccgatcgat
     5161 ctcggcggcc agagccacca tgcgagcatc agacgcattg aacgactggg cagctcgaac
     5221 ctgtcgtcga taccgatcgg cttgcgcccc tgcctgcagt gcttcaatgg taggcaacgc
     5281 cacacgttcg ccttccatgc catgaatagc ggtttccaca tagaccgtgc ggctccagag
     5341 gccaccagca agcagaatca gcagccccac gtgaatcaga gcccccccga tcagcgccag
     5401 tcggccaacc ggtctgaaat gcgtaacgag cccgtcgtcg aacgtccggc tctgccaccc
     5461 cgtatctcgt ttgagaagaa cctttgtcgg gtcgagttcg acgcgcaccg gcagagaaag
     5521 aatctcgcgg ggggagagat aatcgagacg ctgaaacaga cgctggcggg tcagcttgag
     5581 acgggtcagc gtgcacaaca gcgtcgagag cgccagaact gccagcagac cgaa
//
LOCUS       DAORHD010000116         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14661_length_5633_cov_3.953926, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000116 DAORHD010000000
VERSION     DAORHD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14661_length_5633_cov_3.953926"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKO06_03690"
     CDS             <1..1041
                     /locus_tag="PKO06_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV68774.1"
                     /translation="VLEIEVLLEMILEMTGRVLGGVRSSSLILFDEETGRLQVKVHKG
                     VQRNLVNKTIKIGEGIAGKVFERGQPLLITTPKSDDEMTDQVTNTSIKSSICVPLRAK
                     ENSIGVLAVSDKLSGAAFDENDLEMLSTLGAQIAIAIHNAKLYEDLEASYLAAVRALA
                     NSLDAKDTYTRGHSERVALYSVAIGRRMNLNAEDIRTLQIGSLLHDIGKIGISEAIIR
                     KPTRLSDDEYELIKTHPVQGASIIEPAKFLRTKIPLVKYHHERYDGKGYPEGLVGEGI
                     PLLARIVCVADSYDAMTSKRAYRDNIGTDHAVQELLKHSGTQFDPKVVGVFLELLKDP
                     HFLEEVAKTTVQ"
     gene            1038..3203
                     /gene="recJ"
                     /locus_tag="PKO06_03695"
     CDS             1038..3203
                     /gene="recJ"
                     /locus_tag="PKO06_03695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV68775.1"
                     /translation="MKRQWRIPEIPADALAQLARETGLRELLARILWNRGLRSRALVD
                     RYFEPRRRFLASPFLLEGMYPALHRILKALEGEEKIRIFGDRDVDGITSTVLLYETIK
                     SYTRHVDYAVPVIEDGYGLNQEFLNSARREGVSLIITVDCGISNNREVEEARKLGIDV
                     IITDHHETPPTLPQAISVLDPKAPDSRYPHKELAGVGVAFKLALALTLAQSKSLPVPL
                     VAFFRDAEEISAVRFTPREGFSHVRNLNRQALASAGFLFWSTADREEILPLLPKELRS
                     AQSYILQELQIKHTPGESHRSAADLAADMNIPATYSGAERLVLLYLKFLEAEEPAVAT
                     LWQRALDVLTIGTIADMAPLRGENRTISHMGLKFITHTKRQGLQELFHLLHWKNRAIS
                     ERDVSFTIAPILNSSGRLRSAELAISLLTTDFPFRAKDLAQELYELNQERKRVAEDCY
                     QQIRKLLLAQNDVEKDRLLVVAAPMPNQGVTGIVATRLMIDFSRPVIVLLEDQKRMLG
                     SARGFKQVNIMNALNACADLLEKYGGHLGAAGLTLPAVNLEEFRRRVKAFAALHIQPE
                     ELVAEWLIDAELSIDQVDDELMRELQRFGPFGIDNPTPLFLVRNARFYEVRKVGENRN
                     HLRFRFRKSTGQVLSGIGFNLAAALATDVAGDGTCDIVFSVDANDYNGTRTAQAVIQD
                     VLFHEAARPVTTATPDAAPEATTSTEPLPPEPLPTEVIV"
     gene            3200..3508
                     /locus_tag="PKO06_03700"
     CDS             3200..3508
                     /locus_tag="PKO06_03700"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNV68776.1"
                     /translation="MSIRRNPLADHYDFTRLENMWVSMIGEIIEELIATGELCDCAEC
                     VLDVSAIALNKLPTRYWVSGEFNAFTSPDSFYADPRNREQAKKAVLDAVKLVQSNPHH
                     "
     gene            complement(3518..4849)
                     /locus_tag="PKO06_03705"
     CDS             complement(3518..4849)
                     /locus_tag="PKO06_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68777.1"
                     /translation="MTSKPEDGRCETSLLWAWGALLWLLLCAPSCSAAESVWFRWVGT
                     WGTPGHEAGQLLEPKAMAFSPDGQRLAVSDTANNRVVILRVNRESPLAAVFPTKRHTG
                     VVASFTCEKILGDIWPFEGSTTPHDWPDRYHETDHSDGLTPRRELKGRLYQGGQARIR
                     RWDRVPMDRFSLPQGLEWKDARTLIVCDTGNHRLKVVTLDNEVLTIIGQEGWKDGYFH
                     HPIGIDLDAEGNLYVTEPRSQYIRGLGLDFAQRQRAQGNRVQVFSPAYKFQQRWGHMH
                     HMSGRRWRQFKDPTRIHLTRDGTVYVTDSGNHRVLVCGKDGRPDRVLQRWPYYRLRYP
                     QAVDSDDSGLLAIADTGNHRVLILEGDQLLQIIGGFGVENGRFVEPREARFGPDGSLY
                     VLDSNNCRIQIFERVGGKPPLPVEPLPPPPAATAPVPLPPGPPESAPADSE"
     gene            4963..>5633
                     /locus_tag="PKO06_03710"
     CDS             4963..>5633
                     /locus_tag="PKO06_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68778.1"
                     /translation="MAGNQTYAKRYFEAAVDFFEKGDYGKAVEHINKAIEKAPNDAEI
                     ISTKGVFLHRMNDLQGAVEAYRQAIRSCPQHQFSHFNLGLIYMKMGNATQAVQEWEAV
                     IRLNPNDVDALFNIAVALAQMNKRSEAMLFYQRILKLNSGHVQTHQNLGLMYRDEKQY
                     EKARHHLTRLKELDSTYSEVVNREIVRCAEQEFLDKLAASSAGKVATEHLDGERPDDA
                     HSRGT"
BASE COUNT         1200 a         1756 c         1616 g         1061 t
ORIGIN      
        1 gtgctcgaaa tcgaagtgct gctcgagatg attctcgaaa tgaccgggcg ggttctcggc
       61 ggcgttcgtt cgagttcgct gattctgttc gatgaagaga ccggccggct gcaggtcaaa
      121 gttcacaagg gcgtgcaacg caacctggtc aacaagacca tcaagatcgg ggaaggtatc
      181 gccggcaaag tcttcgagcg cgggcaaccg cttctgatca ccacgccgaa atcagacgac
      241 gagatgaccg atcaggtgac caacacgtcg atcaagagtt ccatctgcgt gcctctgcgg
      301 gccaaagaga acagtatcgg cgtgctggcc gtctcggaca aactctcggg cgcggcgttc
      361 gacgagaacg atcttgagat gctctcgacg ctcggagcgc agatcgccat cgccattcac
      421 aatgcgaaat tgtacgaaga cctcgaagcg agctacctgg ccgcggtgcg cgctctggcg
      481 aactcactcg acgccaagga cacctatacg cgcggtcaca gcgagcgggt ggctctgtat
      541 tccgtcgcga tcggccgccg catgaatctg aacgccgaag acattcgcac gctgcagatc
      601 ggctcgctgt tgcacgacat cggcaagatc ggcatttcag aggcgatcat tcgcaaaccg
      661 acgcgacttt ccgacgatga gtacgagttg atcaagacgc atcccgtgca gggggcctcg
      721 atcatcgagc ccgcaaagtt cctgcgcacc aagattccgc tggtgaagta tcaccacgag
      781 cgttacgacg gcaaaggcta cccggaagga ttggtaggcg aaggtattcc gcttcttgca
      841 cgcattgtct gtgttgctga ctcgtacgat gccatgacca gcaagcgtgc ctatcgcgac
      901 aatatcggca ccgatcacgc cgttcaggaa ctgctcaaac actccggcac ccagttcgat
      961 ccgaaggtgg tcggcgtgtt tctcgaactg ctgaaagacc ctcatttcct ggaggaagtc
     1021 gccaagacca ccgtgcaatg aaacgacaat ggcgcatacc tgaaatcccc gccgacgctc
     1081 tggcccaact tgccagagag accggtctgc gcgagctgtt ggcccgcatt ctctggaatc
     1141 gcggtctgcg cagccgggca cttgtcgacc ggtattttga accacgccgg cgctttctgg
     1201 cctcgccgtt tctcctcgag gggatgtacc cggcgctgca ccgcattctt aaggcacttg
     1261 agggcgaaga aaagattcgc atcttcggag atcgcgacgt cgacggtatc acgtcgaccg
     1321 ttctcctgta cgaaaccatc aagtcgtata cccggcacgt cgactatgcc gtaccagtca
     1381 tcgaagacgg atatggtctc aatcaggagt ttctgaactc ggcgcgacgc gaaggtgtct
     1441 cattgatcat cacggtcgat tgcggcatca gcaacaaccg cgaagtcgaa gaggcacgca
     1501 agcttggtat cgacgtcatc atcaccgacc atcacgagac gccgccgaca cttccccagg
     1561 ccatctcagt gctcgacccg aaagcccccg acagccggta tccgcacaag gaactggcgg
     1621 gagtcggtgt ggcgttcaag ctggcgctgg ccctgaccct ggctcagtcg aaaagtctgc
     1681 cggtaccgct ggtggcgttc tttcgcgacg ccgaagagat ctccgcggtg cgattcacgc
     1741 cgcgagaggg gttcagccat gtccggaacc tgaatcggca ggcgcttgcc agtgccgggt
     1801 tcctgttctg gagcaccgcc gaccgggagg agattcttcc cctgctgccc aaagaactgc
     1861 gttcagccca gtcgtatatt ctgcaggaac ttcagatcaa gcacactcca ggcgagagcc
     1921 atcgttcggc cgccgatctc gcggccgata tgaacattcc cgccacctat tccggtgccg
     1981 aacgactggt actcctgtat ctgaagtttc tcgaagccga agaaccagcc gtcgcaaccc
     2041 tgtggcaacg cgctcttgac gtgttgacga ttgggacaat cgccgacatg gcaccgttgc
     2101 gcggcgagaa tcgcaccatc agtcacatgg gactgaagtt catcacgcac accaagcgcc
     2161 aggggctgca ggaactgttt catctgctgc actggaagaa ccgcgccatc tctgagcgcg
     2221 atgtcagctt cacgatcgcc ccgattctga actcctcagg gcgtctgcgt tcggccgaac
     2281 tggccatcag cctgctgaca accgactttc ccttccgcgc caaagacctg gctcaggaac
     2341 tgtatgaact caaccaggag cgcaagcggg tggccgaaga ctgttatcag cagatccgca
     2401 aactgctgct ggcacagaat gatgtcgaga aagatcgact gctggtcgtc gccgctccca
     2461 tgcccaacca gggagtgacc ggcattgtgg ccacgcgact gatgatcgat ttcagccgcc
     2521 ccgtcatcgt gctgcttgag gatcagaagc ggatgctggg ctcggcccgc ggcttcaagc
     2581 aggtcaacat catgaatgcc ctgaacgcct gcgccgatct gctcgagaag tacggcgggc
     2641 atctcggggc cgccgggctg accctgccgg ccgtcaacct cgaggaattc cgtcgccggg
     2701 tcaaggcctt cgccgccctc catattcagc ccgaggaact ggtagccgag tggctgatcg
     2761 acgctgagct ttcgatcgat caggtggacg acgagctgat gcgcgaactg cagcgcttcg
     2821 gccccttcgg tatcgacaac cccaccccgc tgtttctcgt gcggaacgcc cgcttctatg
     2881 aagtgcgcaa ggtgggcgag aatcgaaatc acctgcgttt ccgctttcgg aaatccaccg
     2941 gacaggtcct ttccggcatc ggtttcaacc ttgccgcggc tctggccacc gacgtggccg
     3001 gcgacggcac ctgcgacatc gtcttttcgg tcgacgccaa tgactacaac ggaacgcgca
     3061 ccgctcaagc cgtgattcag gacgtactct tccatgaagc ggcccggccc gtcacgacgg
     3121 cgactcctga tgccgcaccc gaagcgacga cttcgaccga gccgctgccc cccgaacccc
     3181 ttcccactga ggtaatcgta tgagcattcg tcgcaacccc ctcgccgacc actacgactt
     3241 cacccggctc gaaaacatgt gggtcagcat gatcggggaa atcatcgaag agctcatcgc
     3301 caccggcgaa ctgtgcgact gcgcggaatg tgtgctcgat gtgtcggcca ttgccctgaa
     3361 caaactgccc acccggtatt gggtctcggg cgagttcaac gcgtttacgt cacccgactc
     3421 attttacgct gatccacgca atcgcgaaca ggcgaaaaaa gccgttctcg acgccgtcaa
     3481 gctcgtacaa agcaacccgc atcactgagg cacggcttca ctcgctgtcg gcgggtgcgc
     3541 tttccggagg cccgggcggc agaggcacgg gagccgtcgc ggcaggtggc ggcggcaacg
     3601 gctccacggg gagcggcggt ttgccgccga cacgctcgaa tatctggatt cggcagttgt
     3661 tgctgtcgag aacgtacaag gatccatcgg gtccgaaacg cgcttcacgc ggttccacaa
     3721 agcgaccgtt ttcgacaccg aacccgccga tgatctgcag cagctggtcc ccttccagaa
     3781 tgaggacccg atggttcccg gtatcggcaa tggcgagcag ccccgaatca tcagaatcga
     3841 ccgcctgggg ataccgcaga cgataatacg gccagcgctg cagcacgcga tccgggcgcc
     3901 catccttgcc gcacaccaga acgcgatggt tgccggaatc ggtgacgtag acggttccgt
     3961 cccgcgtcag gtgaatccgg gtcggatcct tgaactggcg ccagcgccgg cccgacatgt
     4021 ggtgcatgtg cccccagcgt tgttgaaact tgtaggccgg cgaaaacacc tgcacgcgat
     4081 tgccctgggc ccgctggcgc tgtgcgaagt cgagccccag accgcgaatg tactgagatc
     4141 ggggttcggt gacgtacagg ttcccttcgg catccagatc gataccgatg gggtgatgga
     4201 aatagccgtc cttccagcct tcctggccga tgatggtcag cacctcgttg tcgagggtga
     4261 cgaccttgag gcgatggttt ccggtatcgc acacgatcag ggtgcgcgcg tccttccatt
     4321 cgagaccctg cggcagcgag aatcgatcca tgggcactcg gtcccagcgc cgaattcgtg
     4381 cctgaccgcc ctggtacaaa cgccccttga gttcgcggcg cggcgtcaga ccgtcactgt
     4441 gatcggtctc gtgatagcga tccggccagt catgcggcgt cgtgctgcct tcgaagggcc
     4501 agatgtcgcc gagaatcttc tcgcacgtaa agctcgccac cactcccgtg tgccgtttcg
     4561 tgggaaagac cgcggcaaga ggcgattcac ggttcacgcg caggatgacg acgcggttgt
     4621 tcgcggtgtc actgacggca agacgctgtc cgtcaggaga aaaggccatc gccttgggtt
     4681 ccagcagttg gccggcttcg tgtccgggtg tgccccacgt accaacccag cgaaaccaga
     4741 ccgactcggc cgccgaacag ctcggcgcac acagcagcag ccagagaagc gcaccccaag
     4801 cccaaagaag gctggtctcg caccgtccgt cttccggttt gctcgtcatc gaaccgcgtg
     4861 tcagggggaa tcgcatgggc ggtattttac tctacccggg cgaaaatttg aattcatccc
     4921 gttcattggc tataatacgc acactccgca ggggcaggtg ttatggcagg caatcagaca
     4981 tacgcgaaac gctacttcga ggctgccgtc gacttcttcg aaaaaggcga ctacggcaag
     5041 gccgtcgaac atatcaacaa agccatcgag aaagccccca acgacgccga gatcatttcc
     5101 accaaaggcg tgttcctgca tcgcatgaat gatctgcagg gcgccgtgga agcctatcgt
     5161 caggcgattc gttcctgccc ccagcatcag ttcagtcatt tcaatctcgg gttgatctac
     5221 atgaagatgg gcaatgcgac acaagccgta caggagtggg aagcagtgat tcgcctcaac
     5281 ccgaacgacg tcgacgccct cttcaacatt gccgtcgctc tcgcccagat gaacaagcgc
     5341 agcgaagcga tgctcttcta tcaacgcatc ctgaaactca attccgggca cgtgcagacg
     5401 catcagaacc tcggtctcat gtaccgcgac gagaagcagt acgaaaaagc ccgacaccat
     5461 ctgacccggc tgaaagagct cgactccacg tattccgagg tcgtgaaccg agaaatcgtg
     5521 cgctgtgccg agcaggagtt tctcgacaaa ctggcggcct cgagtgccgg aaaggtggca
     5581 accgagcatc tggacgggga aagacctgac gatgcgcatt cacgaggcac tca
//
LOCUS       DAORHD010000117         5619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14710_length_5619_cov_3.802660, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000117 DAORHD010000000
VERSION     DAORHD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14710_length_5619_cov_3.802660"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PKO06_03715"
     CDS             complement(<1..325)
                     /locus_tag="PKO06_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68779.1"
                     /translation="MNTLTVTLLSQETECGTECAVRFDVNGISLLDQLRSYEERFALR
                     EGHPSLAGSYSWLPATTHTRQSLQLAMSCHRAGAPTALLECACGVPGCWPMLATITYQ
                     DATVTW"
     gene            complement(358..2178)
                     /locus_tag="PKO06_03720"
     CDS             complement(358..2178)
                     /locus_tag="PKO06_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV68780.1"
                     /translation="MRGLSISTKILLGFVFILLLSITAMILANVNLAKLKVRIEFMRE
                     VQFPIMQQLQEMVAESTSASVTFMQIITQGSNDDLKNAFQVSTDNALSLLNSLSELAG
                     NPGIKNPDAIRISMTETRELIEGMVTRANGIMQSAMPLPAASYTEILVYIRDHQEAIR
                     AKSNAVAKYFEDTEGRIIQVDLANIFGKIGTIMKFLPGIIIIFSLGFTIFISTGITGP
                     LKKLSQSMGAAERGMLETRIELKSNDEFQSLAESFNRMLSGICQIIAKVMETSNDLAT
                     SSQELSSASVESAATLIDISKNVNQINSSTADISNNLDQTAHSIDSFADSAHQVASLA
                     ETAVEAVTVTTEAAYKGGQTVKKSVEMIGKIKESVDVATQVILDLNTTSGQIGEIVNT
                     ITTIASQTNLLALNAAIEAARAGEQGKGFTVVAEEVRKLAEESAEAAEAIGTRIENIL
                     TKTQNAVDSMQLGRTRVDEGIRVIREVSLSLDNINNSINDVNKKIKDISKISGEQSRG
                     SEVMSRTVEDIAKLTRTTSERTSVVATSVEQQSATVSQISNSTEDLARLADELHTLVS
                     RFTILSDQKAFPRAERPQLGGPGKTPSPSQKASTPPARNQ"
     gene            complement(2178..3263)
                     /locus_tag="PKO06_03725"
     CDS             complement(2178..3263)
                     /locus_tag="PKO06_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HNV68781.1"
                     /translation="MILDDSIRLMLEEQERQTLSPHACQSSQSRGRRVAEKPCFIRTA
                     FQRDTDRIIHSEAFRRLKHKTQVFLAPTNDHFRTRMTHTLEVVGVARTIARGLRLNVD
                     LAEAIAYGHDLGHTPFGHAGEQVLNEISPQRFHHATHSLRVAEQLEKNGRGLNLSLEV
                     LDGIVKHSKGRKGALFTEEGPDAPLTLEAAIVRISDLIAYLNHDIDDAMRAGLITVSD
                     LPPEATQLLGSRHAQRIHTMVKDIISTSRETCKVRMSGEVWEATEQLRAFLYEKVYPR
                     PEIESEIQKSKALLRQMADWFLEHPNALMKHLKHAPPPEQPLVQTLVDYLASMTDAFA
                     ISIFKELFLPHYRLDMSLIPRAAGGPA"
     gene            complement(3318..4625)
                     /locus_tag="PKO06_03730"
     CDS             complement(3318..4625)
                     /locus_tag="PKO06_03730"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNV68782.1"
                     /translation="MRTYDVIKNKRDGHKLSPEEIRFLIAGFVNGEIPDYQMAAWLMA
                     VFFKGMDEEETFHLTDAMRRSGDLIELPGIQGFTIDKHSTGGVGDKTSLVLGPVLAAL
                     GLKVAKLSGRGLGHTGGTIDKLEAIKGFSCDLSTPKFIDAVNRIGVAIAGQTGNLVPA
                     DKKIYALRDVTATVDSIPLIAASIMSKKLAIHNHGLVLDVKVGSGAFMKSLPDARELA
                     KLMVKIGRQDGRQVTAVLSNMDEPLGEMVGNALEVKEAVNTLRGKGPSDLMELVRNLA
                     TESLLLARDRKVTQAEASQAVSEVITNGKAFAKLIDMVKNQGGDPAMVEDLSRLPAAK
                     EILPLKANCDGYVKQIACEEVGLAAMMLGAGRETKESVIDPAVGFRIVKRVGDKVKQG
                     DTLAEVHVNPGHNNDAAMQRLLSAYIIGSEPVKPMALVLDIVS"
     gene            complement(4671..4871)
                     /locus_tag="PKO06_03735"
     CDS             complement(4671..4871)
                     /locus_tag="PKO06_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68783.1"
                     /translation="MTVPGVPTGPLPKCFGTDYFGKWNTTGMTFDSMKVDKPDQQQKC
                     AECPYFERCCAVNNIKILRIKR"
     gene            5059..5316
                     /locus_tag="PKO06_03740"
     CDS             5059..5316
                     /locus_tag="PKO06_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68784.1"
                     /translation="MAKAKTIKKAGTPAVETPLKKKDKATGTRRDYDPKEIYEEGETV
                     YHKIWDDEGEIIEVGTTEDGIRKIRVQFGKVGLKTLCMGQG"
BASE COUNT         1141 a         1440 c         1710 g         1328 t
ORIGIN      
        1 tccaggtgac cgtggcatcc tgatacgtga tggttgccag catcggccaa caaccgggca
       61 caccacaggc gcattccagc agcgctgttg gtgctcccgc acggtgacag gacatcgcca
      121 actgcaggga ctgtctggtg tgagtggtgg cgggaagcca cgaatagctg ccggcgagtg
      181 acgggtgacc ttcacgcagt gcgaaacgtt cctcgtatga tcgcaattgg tctaacagac
      241 tgatgccatt gacgtcaaaa cggacggcac attccgtacc acactcggtt tcctgtgaga
      301 gaagggttac ggtcagggtg ttcatgagag aacccggtgg ttgcgaagag gccagttcta
      361 ctggttcctg gcgggcggtg tggaagcttt ctgagaaggt gacggcgttt tgcctggacc
      421 accaagttgg ggtcgctcgg cgcggggaaa tgccttctgg tcgctgagaa tcgtgaagcg
      481 ggagaccagc gtgtgcagtt cgtcggcgag tcgagccagg tcttccgtgg agttcgaaat
      541 ttgagacacc gttgccgact gctgctcgac cgaggtggcg accacgctgg tgcgctcgga
      601 tgtcgtgcgg gtcagcttgg cgatatcttc aaccgtgcga gacatgactt ctgaaccacg
      661 agactgctcg ccggagatct tgctgatatc tttgatcttc ttgttcacgt cattgatgct
      721 gttgttgatg ttgtcaagac tcagactgac ttcacgaatg acgcggattc cttcgtcgac
      781 tcgggtgcgt ccgagttgca ttgaatcgac ggcattctgc gtcttggtca gaatgttttc
      841 gatgcgggtt ccgatggctt cagcggcctc tgccgattct tcagccagtt tgcgcacttc
      901 ttcggcgaca accgtgaagc ccttcccctg ctcaccggcg cgggctgctt caatagcggc
      961 gttcaacgca agcaggtttg tctgactggc gatggttgtg atcgtgttga cgatttcgcc
     1021 gatctggcct gaagtggtgt tcagatcaag aatgacctgc gtggcgacat cgacggactc
     1081 tttgattttg ccgatcatct cgaccgactt cttcacggtc tgaccgcctt tgtaagccgc
     1141 ttcggtggtg acggttaccg cttcgacggc ggtttccgcc agcgaggcga cctggtgtgc
     1201 cgagtccgcg aatgaatcga tcgaatgcgc ggtctgatca agattgttgg agatgtcggc
     1261 ggtggaagag ttgatttggt tgacgttctt ggaaatatcg atcagcgtcg cggctgattc
     1321 cacggaagcg gaactgagtt cctgagagct ggtcgccaga tcgttgctgg tttccatgac
     1381 cttggcgatg atctggcaga tgcccgaaag catgcgattg aaactctcag cgaggctttg
     1441 aaactcgtca tttgatttca gttcgatgcg ggtctcaagc atgccgcgct cggcggcccc
     1501 catggattga gacagtttct tcaagggtcc ggtaatgccc gtagaaatga agatcgtgaa
     1561 tccgagactg aagataatga tgatgccggg cagaaacttc atgatcgtgc cgatttttcc
     1621 gaaaatgttg gcgagatcga cctgaatgat tcggccttcg gtgtcctcga aatacttggc
     1681 taccgcattc gacttggcgc gaatggcttc ctggtggtcg cgaatataca caagaatttc
     1741 ggtgtaactc gcggcaggca gtggcatggc gctttgcatg attccgttgg cacgggtgac
     1801 cattccttcg atgagttctc gggtttcggt catcgagatg cggatggcat cggggttctt
     1861 gatgcctggg ttgccggcaa gctcagagag ggagttcagc agagacaagg cgttgtcggt
     1921 ggaaacctga aaggcattct tcaggtcgtc gttgctgccc tgggtaatga tctgcatgaa
     1981 agtcaccgag gcagaggtgg attccgcgac catttcctga agctgctgca tgatggggaa
     2041 ctgaacttcg cgcatgaatt cgatgcggac tttcagcttg gccagattga cgtttgccag
     2101 gatcatggcc gtgatactga gcaaaagaat aaaaacaaag ccaagcagaa ttttggtgct
     2161 gattgaaaga ccgcgcatca ggctgggcct cccgcggcac gggggatgag ggacatgtcg
     2221 aggcggtagt gtggcaggaa gagttccttg aagatgctga tagcgaaagc gtcggtcatg
     2281 ctcgcgaggt aatcgacgag cgtttgtacc agtggctgct caggcggagg cgcatgtttc
     2341 agatgtttca tgagagcgtt cgggtgttcg agaaaccagt cggccatctg gcgcagaaga
     2401 gctttcgact tctgaatctc gctttcgatc tcgggacgag ggtaaacctt ttcatacaag
     2461 aacgcccgca gttgctcggt ggcttcccag acctcgccgc tcattcgcac tttgcaggtt
     2521 tcgcgactgg tactgatgat gtccttcacc atggtatgaa tgcgctgggc atgacgtgag
     2581 ccgagcagtt gggtggcctc gggtggcaga tcgctgaccg tgatgagtcc cgctcgcatg
     2641 gcatcgtcga tgtcatgatt gagatacgcg atcagatctg aaatacgaac gatagcggct
     2701 tccagggtca gcggagcgtc tggaccttcc tcggtgaaca gcgcgccctt ccgccccttg
     2761 ctgtgtttta cgataccatc gaggacctca agggaaagat tcagcccgcg accatttttc
     2821 tcgagttgct cggctacccg cagactgtgc gtggcatgat gaaagcgctg tggactgatt
     2881 tcgttgagca cctgttcgcc ggcatgtccg aatggggtgt gtccaagatc atggccgtag
     2941 gcgatcgctt cagcgaggtc gacgttgagc cgcaatcccc gggcgatggt ccgggcgacc
     3001 ccaaccacct cgagggtatg ggtcatgcgc gtgcgaaaat ggtcatttgt cggcgccaga
     3061 aagacctggg ttttgtgctt gagccggcgg aaggcttcgc tgtgaatgat gcgatcggta
     3121 tctcgctgga atgccgtgcg aatgaagcag ggtttttcag caacgcgccg gccacgagac
     3181 tgcgatgact ggcaggcatg cggcgacaga gtctgcctct cctgctcctc aagcatcagg
     3241 cgaatactgt catcgagaat catgccgcgg tcgtgccttt gtcccagttt gaattttgct
     3301 tcaatcgcat gaattaatta tgagacgata tcgagaacca aagccatcgg tttcactggc
     3361 tcgctgccaa tgatgtaagc tgagagcagg cgctgcatgg ccgcgtcatt gttatgcccc
     3421 ggattgacgt gcacttccgc gagcgtgtcg ccttgtttca ccttgtctcc aactcgtttc
     3481 acgatacgga acccaacggc gggatcgatg accgattctt tggtctcgcg cccggccccg
     3541 agcatcatgg ccgcgagccc gacttcttca caggcaatct gcttcacgta accgtcacaa
     3601 ttcgctttga gaggcagaat ctctttggcc gccggcaacc gtgacagatc ttcgaccatg
     3661 gctgggtcgc ccccttgatt cttgaccatg tcgatcagtt tggcgaaggc cttcccgttg
     3721 gtgatcacct cgctgacagc ctgagaggct tcggcttggg tgactttgcg gtcacgggcg
     3781 agcaacaacg attcggtagc gagattgcgt accagttcca tgaggtcgga aggccctttg
     3841 ccacgcagag tattgactgc ttctttgact tcgagggcgt tgccgaccat ttcgccgagc
     3901 ggctcgtcca tgttcgacag tacggcggtg acctggcgtc cgtcctggcg accgattttc
     3961 accatgagct tggccagctc gcgtgcgtcg ggcaggcttt tcatgaacgc gccgctgccg
     4021 actttgacgt cgagtaccag gccgtgattg tgaatcgcga gtttcttgga cataatcgag
     4081 gctgcgatca gcggaatcga gtcgactgtg gccgtaacgt cacgcagagc ataaatcttt
     4141 ttgtcggcgg gaacgagatt gccggtctgt cccgcaatcg caacgccgat gcggttgaca
     4201 gcatcgatga acttcggtgt ggacaagtcg cagctgaatc ccttgatggc ttcgagcttg
     4261 tcgatggtgc caccggtatg cccgagaccg cgacccgaca gtttggcgac tttcagtccg
     4321 agggcggcca gcacggggcc gagcaccagg ctggttttgt ctccaactcc accggtcgag
     4381 tgtttgtcga tcgtgaaccc ttggatgccg ggcagctcga tcagatctcc gctgcgccgc
     4441 atcgcgtcgg tcaggtggaa ggtctcttct tcatccatgc ccttgaaaaa caccgccatc
     4501 aaccaggcag ccatctgata atcgggaatc tcaccgttca caaacccggc aatgagaaag
     4561 cggatctctt ccggggacag cttgtgacca tcacgcttgt tcttgatgac gtcgtatgtg
     4621 cgcatgagtg cctccgttgg ggtgttctga gctgaacgaa acgggccgga tcagcgcttg
     4681 atgcgcagga tcttgatgtt gttcaccgcg cagcagcgct cgaaatacgg acactccgcg
     4741 cacttctgct gctggtcagg cttgtcgacc ttcatcgagt cgaaggtcat gccggtggtg
     4801 ttccacttgc cgaaatagtc tgtgccaaag cacttgggaa gagggccggt cgggactccg
     4861 gggacagtca tttgtcatct cctggggcgg attgagaatc ggtctgtcgt tcgaacacga
     4921 cagccgcgtg ggtaaaatcg ctggcaattg tagcacacat tcgggagatc aaccaccatc
     4981 tctggggcta tttgacttga cccgagaact gtggtaccct gctgccacta atcccgacag
     5041 ttggagggta ctcccattat ggcaaaagcc aagaccatca agaaagccgg aactcccgcg
     5101 gttgaaacac cgttgaaaaa gaaagacaaa gcgaccggaa cccgccggga ctacgatccg
     5161 aaagagatct acgaagaagg cgaaaccgtg tatcacaaga tctgggacga cgaaggagag
     5221 atcattgaag tcggtacgac cgaagacggc atccgcaaga ttcgggtgca gttcggcaag
     5281 gttggcctga aaaccctgtg catgggtcag gggtaggcag cgcacagagc cgctgttgag
     5341 agttgcaggc acgcgattgt tgagcatgtt ttgacatcgt ggttttgacg tcggtttgaa
     5401 tgacgcaccg gcacgttacg gaagaaaaac ttccgctctc gtgccggtgt ttctttgttg
     5461 ccagatctgt ggtggtcggt ttcttgggcg atggcacttt gatgtggagc atttctctgg
     5521 gctgaagtgg agagaggaga gacgatcccg aaaccttcgg aggtagtggg aatctgccga
     5581 tagagaggtg attccttgtt tgaggggagg gttgtcggc
//
LOCUS       DAORHD010000118         5617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14715_length_5617_cov_3.987415, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000118 DAORHD010000000
VERSION     DAORHD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14715_length_5617_cov_3.987415"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PKO06_03745"
     CDS             complement(<1..1531)
                     /locus_tag="PKO06_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68785.1"
                     /translation="MEYRSTSSSSGSGATSPVPWVPWRLLLCLLLPTLLLSWGASSRT
                     QAALDRHLEHLQTGMDLRLDQLVPFVDEGYDLHRKLSALCDRAEAAPDPFIRLKHELP
                     RFNQRYHQAFRFFVWDASGEVVKELTDQAGYRYVLRQLWETLHEVARSHLSAVPQPPL
                     SLEIVQRNSTLLRSFIGPFLAMNHLTRPLTTGQLHPVPLAHAQPDHSRFWYRLGRRFS
                     VFCLIGQEPFRRPRSLRATIQSHNGSRQPLFQLGLFHEIDRRLEVPGGVVSHRSEVKL
                     GLQALQKRLSGRQFTGRLYLTWRNLDVGLWGVAIADVDPFLRRSRADKAWWAALGVVF
                     GFLAWASLTLSEWRGSGASVRHRIITLFLAANGLPLLTLGIWSADYLQQKEKALINEA
                     FIEGEKALRTFDSSLHLFLQSLERTSNRLLSPFTETFSVPLEPQTLQDLHQVFARVGG
                     DQLYILASGTGDLIDRVGISNDDRLRAIETMGIAYAYAALLIANHGSRLPPLPPVSQR
                     GI"
     gene            complement(1617..2717)
                     /gene="ricT"
                     /locus_tag="PKO06_03750"
     CDS             complement(1617..2717)
                     /gene="ricT"
                     /locus_tag="PKO06_03750"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNV68786.1"
                     /translation="MSRYASFRLRKLPMVHWFSIPDQLNLSPRQQVLTRTQHGTEIAT
                     VLRVLPDDRSLEQIPEPFIQELIRPLTPEDRKAAKELADREQQAFKRTRELIVQHQLD
                     MKLLKAEYLFDGTRLILYYKAENKVDFRDLLKSLASSFKTRIELRQIGVRDETKLLGG
                     IGCCGKEVCCRQFLGSFHPVTTKMAKDQNLSLNPAKLSGVCQRLLCCLSYEHKYYASF
                     HGRFPKISAEILIGTERARVMDLNFITQKVLVGFMDRRKLYVQLEHIKGRKDPQTGRN
                     LWWVQEPGQPEPDVAILLQPIVPPAERMPRNRKDRPDKPDRPRRDDRRADGNEPRRSD
                     EGAASPTDEEDPVSRDEPAEMTGDAPPTDELS"
     gene            complement(2714..4045)
                     /gene="holB"
                     /locus_tag="PKO06_03755"
     CDS             complement(2714..4045)
                     /gene="holB"
                     /locus_tag="PKO06_03755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNV68787.1"
                     /translation="MSNATPCRLQSLLLQQEAVSQLGHVLRGGRLQQTYLLTGAPSVG
                     KGTLARAFAAALLCEHPDSSATDFPDACGQCDSCRRLETGSHPDVSIISPRGNEIRIQ
                     QVRTMQEDAQLKPALGSWRVFIIDPADRLNEFSANSLLKILEEAPPRVIFLLLASSPE
                     GVLPTILSRSTRVPLRSPSFHEARQALTALTSVPAAQAAEALAICQGRLGFARRLLAV
                     GIPPSPEEPAVVSPADLGHWQAAYFDELARLPALIDERFERSSFVDALWPTLQELLEQ
                     PAPRLAVSEYAFTLALAAAPGLPRAFPILFTGGFLDLLDLIKREAKKSADALLKRLKA
                     GLPSAMLKELEEQFTQWATEFAQRRFVSLLEGLGLAWADGFRYATCGDEEMLLNIGRK
                     DIIMKLAAFPGSAVLESRLQTLERSVQRHRRYIQPALILENILTDIGGPLR"
     gene            complement(4046..5296)
                     /locus_tag="PKO06_03760"
     CDS             complement(4046..5296)
                     /locus_tag="PKO06_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNV68788.1"
                     /translation="MSIIIVDDSKVNRLLLRRILEERGLKDLFEAGDVTELFALLGFE
                     GAGQHPPACQNADLILMDIMMPDIDGIEATRRLHASQSFHDTPVIMVTAAGEGELLER
                     AFAAGAMDYLTKPIDATELVARVKSALTLKREMDTRKARERELLELQNKLVLSNDHLN
                     NVLKQIQADIELAAQTQIRMLPPQGAAFPSLQSHWQLLTCSRLGGDFLNLIPLSPRQT
                     AFLLVDVSGHGIQAALLAFSLGHLLANTGGDSLLTSREGTPRQPGEMLAMLNQRFCTE
                     EELGQYFTIAYGVFDAETRTVTISRAGHPPAIHLAAATGTARALREGGPPIGMVDNID
                     FPEDRFTVQPGDRLFLYSDGITETAAQDTGVLLGESGLEQILVRQHTQPLSVALEQVM
                     QACAQWRGSREQADDMSILGLQFL"
     gene            complement(5293..>5617)
                     /locus_tag="PKO06_03765"
     CDS             complement(5293..>5617)
                     /locus_tag="PKO06_03765"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNV68789.1"
                     /translation="WDTSEGDDEKIALWIGTRNGITRVSRMGGVMTLARPRFTTEAHP
                     FPWPGSPTDLQPIWAYSWYGNPIEDMNDVCPYDGLPGNDITCLALDDLNLYVGTNNGL
                     CRIRK"
BASE COUNT         1113 a         1634 c         1793 g         1077 t
ORIGIN      
        1 gtatgccgcg ctgcgatacg ggggggagcg ggggcagtcg tgagccgtgg ttcgctatca
       61 gcagcgcggc atacgcgtag gcgattccca tcgtctcgat ggcacgcaga cgatcgtcgt
      121 tgctgatccc gacgcgatcg atcagatcgc ccgtgccgct ggcaagaatg tagagctggt
      181 cgccgccgac acgggcaaac acctggtgca agtcctgtag tgtctggggt tcgagaggaa
      241 cggaaaaggt ttccgtgaac ggtgacaaca aacggttact ggtacgctcg agtgattgca
      301 gaaagaggtg cagcgacgaa tcgaaggtgc gcagagcctt ttcgccttcg ataaaggctt
      361 cgttgatcag tgccttttcc ttctgctgca ggtaatcggc gctccagatg ccgagcgtca
      421 aaagaggcag gccattggcc gccagaaaga gggtgatgat gcggtgccgc accgaagcgc
      481 cgctgccgcg ccactcgctg agcgtgagcg aggcccacgc caggaacccg aagacgactc
      541 ccagcgcggc ccaccaggct ttgtcagcgc gcgatcgtcg cagaaacgga tcaacatcgg
      601 cgatggcgac tccccacagc ccgacgtcaa ggttgcgcca ggtcaggtac agacgtccgg
      661 tgaactgcct tcccgacagg cgtttctgga gtgcctgaag gccgagcttc acttctgaac
      721 ggtgcgacac cacccccccc ggaacctcga ggcgacggtc gatctcgtga aacaggccca
      781 gttgaaagag cggctggcga gacccgttgt ggctctgaat cgtcgcccgc aggctgcgtg
      841 gccggcggaa cggttcctga ccgatgagac agaacacgga aaaacgtctg cccaggcgat
      901 accagaaccg cgagtggtcg ggttgcgcgt gtgcgagcgg cacaggatgc agttgtcccg
      961 tcgtcagagg ccgcgtcaga tgattcattg ccagaaaggg gccgatgaac gatctcagaa
     1021 gagtgctgtt ccgctgcacg atctcgaggc tcaggggcgg ctggggcacc gcgctcagat
     1081 gactgcgggc cacttcgtgc aatgtctccc acaactgacg gagaacatag cgatagccgg
     1141 cctgatcggt cagttccttg acgacttccc ctgaagcgtc ccagacaaaa aaacggaaag
     1201 cctgatggta acgctgattg aaacgaggaa gttcatgctt gagccggatg aacgggtcgg
     1261 gagcggcttc ggcgcggtcg cagagagcgc tcagtttccg atgaagatca tacccctcgt
     1321 cgacgaaagg gaccagctga tccaaccgca gatccatgcc ggtctgcaga tgctccaggt
     1381 gtcgatcgag agccgcctga gtgcgcgaag acgctcccca cgaaaggagc agcgttggca
     1441 gcagcaggca gagaaggaga cgccagggaa cccacgggac cgggctcgtg gctcccgatc
     1501 cggaactcga tgacgtactg cgatattcca tgctgagatc cgctcaataa gccaagcatg
     1561 cacacagtcg gctcggacaa tgctcgccga cggttacgcg aagaaaacgg tcgatctcag
     1621 gaaagctcgt ccgtgggcgg agcatcgccg gtcatctcgg cgggctcatc tcgtgatacg
     1681 gggtcttctt cgtcggtggg ggaggctgcg ccctcatccg accgtcgcgg ttcgttccca
     1741 tccgcacgcc gatcgtcgcg gcgtgggcgg tccggtttgt ccgggcggtc tttgcggttc
     1801 ctgggcattc gctcggcggg gggaacgatg ggctgcagca gaatcgccac atccggttcc
     1861 ggctggccgg gttcctgtac ccaccacagg ttacgcccgg tttgcggatc ttttcgaccc
     1921 ttgatgtgct cgagttgtac gtacagcttg cgacggtcca tgaatccgac cagcaccttc
     1981 tgggtgatga agttgagatc catgacgcgg gcgcgttcgg tgccgatcag aatttccgcg
     2041 ctgatcttgg ggaaccggcc gtggaacgat gcataatact tgtgttcgta ggaaaggcag
     2101 cagagcagcc gttgacagac gcccgacagc ttggccggat tcagactgag attctggtct
     2161 ttggccatct tggtcgtgac cgggtgaaaa ctgcccagaa actgccgaca gcagacttcc
     2221 ttgccgcagc acccgatgcc gccaagcagt ttcgtttcat cgcgaacgcc gatctggcgc
     2281 agctcgatgc gtgtcttgaa actggacgcc agactcttga gcagatcgcg gaaatccacc
     2341 ttgttttccg ctttgtaata cagaatcagg cgggtgccgt cgaacagata ctcggccttc
     2401 agaagcttca tatcgagctg atgctgcacg atcagttctc gcgtgcgctt gaacgcctgc
     2461 tgctcacgat ccgccagttc cttcgccgct tttcggtctt cgggcgtgag agggcgtatc
     2521 agctcctgaa tgaacggctc gggaatctgc tcgagagagc ggtcgtccgg aagcacgcgc
     2581 aggaccgtgg cgatctcggt gccgtgttgc gtacgagtca gtacctgctg acgaggggaa
     2641 aggttcagct gatccgggat ggaaaaccag tggaccatgg gaagtttgcg gagtctgaat
     2701 gaagcgtagc ggctcacctg agtgggcctc cgatgtcggt aagaatgttt tcgagaatca
     2761 gggcaggctg aatgtagcga cggtgtcgtt gcaccgatcg ttccagtgtc tgcaggcgag
     2821 attccagaac ggccgagccc ggaaaggctg ccagcttcat gataatgtct ttccggccga
     2881 tattcaacag catttcttcg tcgccgcagg tggcataacg gaatccatcc gcccacgcca
     2941 gacccagccc ttcgagcaga ctgacaaaac gacgttgcgc gaactcggtg gcccactgcg
     3001 tgaactgctc ctcaagctcc ttgagcatcg ccgaaggcaa cccggctttc agccgcttga
     3061 gaagagcgtc cgcgctcttc ttcgcttcgc gcttgatcag gtcgagcagg tcgagaaagc
     3121 ctccggtgaa gagaatcgga aacgcgcggg gcagaccggg agcagcggcc agggcaagcg
     3181 tgaatgcata ctcgctcacg gcgagccgtg gcgcgggctg ctccagaagt tcctgcaggg
     3241 tgggccagag cgcatccacg aaagaggagc gttcgaaccg ttcatcgatc agagcgggaa
     3301 gtcgggccag ttcatcgaag taggctgcct gccagtgccc cagatcagcg ggcgagacaa
     3361 ccgcgggctc ttccggtgac ggcggaatac cgacggcaag aagtcgtcgt gcgaatccaa
     3421 gtcgcccttg gcagatggcc aaagcttctg ccgcttgagc tgccggtact gatgtcagcg
     3481 cggtcagggc ctgtcgtgct tcgtgaaagg acggagagcg gagcgggacg cgggtcgaac
     3541 gactcagaat cgtcggcaag acgccttcgg ggctcgaagc cagcaagaga aaaatgactc
     3601 gtggcggagc ttcttcgaga atcttcagca gactgttcgc cgaaaactcg ttcaggcgat
     3661 cggcggggtc gatgatgaac acgcgccacg aaccgagtgc gggcttcagt tgcgcgtctt
     3721 cctgcatcgt gcgtacctgt tgaatgcgaa tctcgttgcc gcgcgggctg atgatcgaca
     3781 cgtcgggatg gctgccggtt tccaggcgac gacacgaatc gcactggccg caggcatccg
     3841 gaaagtcggt cgccgatgag tcgggatgtt cgcacagcag ggcggccgca aaagcccgcg
     3901 ccagcgtccc cttgccgacg gacggggctc cggtcagcag ataggtctgc tgcagtctgc
     3961 cgccgcgaag cacatgtccc agttgtgaga cggcttcctg ctgcagcaga aggctttgta
     4021 gccgacaggg agtcgcgttg gacatttaga gaaactgcaa cccgagaatc gacatgtcgt
     4081 cggcctgctc tcgcgaaccg cgccactgcg cgcaggcctg catgacctgt tcgagcgcca
     4141 ccgaaagagg ttgggtgtgc tgtcgtacca gaatctgttc gagtcccgat tcccccagga
     4201 gcacgccggt gtcctgggcg gccgtctcgg tgattccgtc cgagtagaga aacagccggt
     4261 cgccgggttg aaccgtgaaa cgatcctcgg gaaagtcgat gttgtctacc atgccgatcg
     4321 gtgggccacc ctcgcgcagc gcgcgagcgg ttccggttgc cgccgcgaga tggatggccg
     4381 gtggatgacc ggcgcgcgaa atggtcaccg tgcgggtttc cgcgtcgaag accccgtaag
     4441 cgatggtgaa atactggcca agctcctctt cggtgcagaa acgctggttg agcatcgcca
     4501 gcatctcgcc cggttgtcga ggcgttcctt cgcgactggt cagcagactg tcgccgccgg
     4561 tgttggccag cagatgtccg aggctgaatg ccagtaacgc cgcttgaatg ccgtgcccgg
     4621 agacgtctac cagcagaaaa gccgtctggc gaggcgaaag cggaatcagg ttcagaaagt
     4681 cgccgccgag ccgggaacag gtcagcagct gccagtggct ctgcagactc ggaaacgcgg
     4741 ctccctgcgg ggggagcatg cgaatctgcg tttgagccgc gagttcgata tcggcctgga
     4801 tctgcttgag aacgttgttc aggtggtcat tcgagaggac cagcttgttc tgcagttcca
     4861 gaagctcgcg ctcccgggct ttgcgcgtat ccatttcacg tttcagcgtc agcgcagact
     4921 tgacccgcgc caccagttcg gtcgcgtcga tgggtttggt caggtaatcc atggctccag
     4981 ccgcgaacgc ccgctccagc agctcccctt ctccggcggc ggtgaccatg atgaccggcg
     5041 tgtcgtgaaa cgactggctc gcgtgcagac gtcgggtcgc ctcgatcccg tcgatgtccg
     5101 gcatcatgat gtccatcagt atgagatcgg cgttctgaca ggccgggggg tgttggccgg
     5161 ccccctcgaa gccgagaagc gcgaaaagtt ccgtcacgtc tcccgcctcg aacaagtcct
     5221 tcaggccgcg ttcttccaga atgcgacgca gcaagagtcg gtttaccttc gaatcgtcaa
     5281 cgataatgat gctcatttgc gtatcctgca cagtccgtta tttgttccca cgtacaagtt
     5341 cagatcgtcg agcgccagac aggtgatgtc gtttcccggc agaccgtcat acgggcagac
     5401 gtcgttcatg tcttcgatcg ggttgccata ccaggagtag gcccagatcg gttgcagatc
     5461 ggtcggcgac cccggccagg gaaagggatg agcctccgtc gtgaagcgcg gtcgggccag
     5521 ggtcatgacg cctcccatcc tgctgacccg ggtaatgccg ttccgggtgc cgatccacag
     5581 cgcgatcttt tcgtcgtctc cctctgaggt gtcccag
//
LOCUS       DAORHD010000119         5579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14872_length_5579_cov_2.990768, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000119 DAORHD010000000
VERSION     DAORHD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14872_length_5579_cov_2.990768"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..569)
                     /locus_tag="PKO06_03770"
     CDS             complement(69..569)
                     /locus_tag="PKO06_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HNV68790.1"
                     /translation="MRLTPLDIYQHEFSRKKIGGLDDAEVHEFLKKVGREYESLYAEN
                     KAQKDQIERLTAQMKDYLELEKTLKQTLISAQKASGDLKNNAEKEAQLIVKEAEIQAE
                     RIIDEARQESEFINKEIREMKRQKRMLKVELRTVLESYLTMLNDDAPAATRPANTVTA
                     PVTEAH"
     gene            complement(573..1811)
                     /locus_tag="PKO06_03775"
     CDS             complement(573..1811)
                     /locus_tag="PKO06_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNV68791.1"
                     /translation="MTALSLARPLAPATQTHEIDRISTPQGPVPRIYDEFWTAKQRQM
                     HSLHYAVSYRASFKPELPDYFIRRLTRVGDTVADPFGGRGTTALQAALLGRRAISNDI
                     NPLSERITSPKLNPVTPQAIEERLAEIPFRAHRDLSKEEDLSMFYHPDTFLELVNLKN
                     YLKRHRDPIDRFIEMVAISRLHGHSPGFFSAYSFPQISIPKANQVKINRTRGVEPEYR
                     DIASRILKKGKSILKDGGVEEIRRSGLISRFTTNDSRKLKDWETGSVNLVVTSPPFLN
                     QVDYIQDSWLEMWFCDIDSDKFRNQLVQTASLDEWKQFISDTLSELHRVLVPGGYLAM
                     EVGEVRHQGELLNLEEVIINMTCNKQYNAGQYRVLQVLVQSQEFTKLANCFQVKNNVL
                     GTNTNRIILMEKIASPVSRR"
     gene            2004..2825
                     /locus_tag="PKO06_03780"
     CDS             2004..2825
                     /locus_tag="PKO06_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HNV68792.1"
                     /translation="MSHEIWSELQEALEAREPFVFCVVTERTGSSPGKPSQKMLVYDD
                     GSTCGTVGGGVNEERVRQKAMELFTSGGSTIMTFELNNPIDGKEPVCGGSMRVYLELM
                     CRQPRLVIFGGGHIGRVLAKLASIAGYRVALADERPAYASPDTLPEVAELLCCPYSEA
                     VTKARIGKDDAVVIVTPGHVHDVEVLRAMLGTPAAYIGMIGSQRKVEGIRRLLLEEGF
                     VSERVNQVFAPIGINLGGETPEEIAVGILAQLVAFNNGQIMKFEAKKRTNVPCPA"
     gene            2944..3918
                     /locus_tag="PKO06_03785"
     CDS             2944..3918
                     /locus_tag="PKO06_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68793.1"
                     /translation="MRKRACWLLIMTFVLSLVVTPQVTFAAGSFLDTLHQTQEGQAED
                     QGLAPSEESTSAAAEPEPAPAEPAPESGQLAFNQGVSIEQLIAQIIQQIYNLLGCRGV
                     PIRIIIIRSGGCTRPPTSGTQPPVTTQPPTTTTPPVTTTPTTPPQGTTDGQGVAGLKA
                     EMAAKYGINAVDGDGAVWSQRQLEEANKVLSTLPAGFRGCTKSIQRDSMFHSPNVLGY
                     VRMGIPTVHMLNSSCREGTFQGTMVHEMTHTFQANHPDITRLWQQTFWPNGRNPSPPS
                     VSSYGNTQPVEDMAEAVRAYWQSGSRMKQTHPDRYEFVKKYIMEGKEF"
     gene            4058..4813
                     /locus_tag="PKO06_03790"
     CDS             4058..4813
                     /locus_tag="PKO06_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68794.1"
                     /translation="MSKKLSLHGVLLLLLVLLACRSGPVWSAENFLETINSVEKQHGV
                     AIERPLKTPAPDSGDPYRIFDDGVAYAKAASATNSVDAWKKKILSKFGIKANDSATAK
                     WSAAQLEKLFSILDKLPASYRQHTKSLVRVKMYKSKYVLGYVKRGIPSVNITNAGCTN
                     SQFTDTIVHEMMHVFLFKNKDKLYSWTSKFWPNGASGKPYPSSVTSYGNTNPEEDLCE
                     SVAFYFSKAKSMKAAQPARYEFIKKFVFFGKEF"
     gene            4815..5294
                     /locus_tag="PKO06_03795"
     CDS             4815..5294
                     /locus_tag="PKO06_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68795.1"
                     /translation="MIRLCLVLVSCCVLGFLAVPGFGMIDTLPLPDLVRQAEMICIAK
                     VVQVNEVSVDKDQVSTVKNVLLSEKLLKGSWQAMEPIVVMTRQSGKYGTVGSLEDQVE
                     FPPKGSHVVLFLKKAKDGSLEPVNLLQGVWPLDGQKPLGMGLGITLQQLEEVVKLNR"
BASE COUNT         1203 a         1416 c         1670 g         1290 t
ORIGIN      
        1 cggccaaaac ggcaagaacg agtccgttct tgccgtcttt cagacaaccg agtgccaatg
       61 gttctgtctc agtgagcttc cgtgacggga gcggtgacgg tgttcgccgg ccgggtcgcg
      121 gcgggcgcat catcattgag catggtgaga tacgactcga gcacggtgcg cagctcgacc
      181 ttgagcatgc gcttctggcg cttcatttcg cgaatttctt tgttgatgaa ctctgattcc
      241 tgtcgagcct catcgatgat gcgctcggcc tgaatctccg cttccttcac gatcagctgg
      301 gcttctttct cggcgttgtt cttgagatcg cctgaagctt tctgcgcgga gatcagagtc
      361 tgcttgagcg tcttttcgag ctccaggtag tctttcatct gcgccgtcag acgttcgatc
      421 tgatctttct gcgccttgtt ttcggcgtag agcgactcgt actctcgtcc gactttcttg
      481 agaaactcat gcacttcggc atcgtcaagg ccgccgatct tcttgcggga aaactcgtgt
      541 tgatagatgt ctaacggggt cagacgcata gattacctcc tgctgacggg cgatgcgatt
      601 ttttccatca ggatgatgcg attggtgttg gtgccgagaa cgttgttctt cacctggaag
      661 cagttcgcga gcttcgtgaa ctcctggctc tgaaccagaa cctggagcac gcgatactgc
      721 ccggcattgt actgtttgtt gcaggtcatg ttgatgatga cctcttcgag gttgagcagc
      781 tcgccctggt gacgaacctc tccgacctcc atggctagat aacctcccgg aaccagcacc
      841 cgatgcagtt ccgacagggt gtcggagatg aactgtttcc actcgtccag actggcggtt
      901 tgtaccagtt ggttgcggaa tttgtcactg tcaatgtcgc agaaccacat ctcgagccag
      961 ctatcctgaa tgtagtcgac ctgattcagg aaggggggcg aggtgacgac caggttgacc
     1021 gaaccggttt cccagtcctt cagttttcgt gaatcgttgg tggtgaagcg cgagatcagt
     1081 ccggatcggc gaatctcctc aacaccaccg tctttgagaa tcgactttcc ctttttgaga
     1141 atgcgcgagg caatgtcgcg atactcgggc tcgaccccgc gagtgcgatt gatcttgacc
     1201 tggttggcct tggggatcga gatctgcggg aaggaatagg ccgagaagaa ccccggagaa
     1261 tggccatgca aacgtgaaat ggcgaccatc tcaatgaatc ggtcgatcgg gtcacggtgc
     1321 cgtttcagat agtttttcag attcacgagt tcgaggaagg tatcgggatg gtagaacatc
     1381 gacagatctt cttctttcga cagatcgcga tgagcgcgga acgggatctc cgcgaggcgt
     1441 tcctcgatcg cctgaggcgt gaccggattc agtttcgggc tggtgatgcg ctcagacaga
     1501 gggttgatgt cgttgctgat cgctcgtcgc ccgagaagag cggcctgcag tgcggttgtg
     1561 ccacgtccgc caaaggggtc ggcaaccgtg tcgccaacgc gggtcagtcg ccggatgaag
     1621 tagtcgggca actcgggttt gaatgaagcc cggtacgaga cagcataatg caaagagtgc
     1681 atctgccgct gttttgccgt ccagaattca tcatagatgc ggggaacagg accctgcggg
     1741 gtggagatgc gatcgatttc atgggtctgg gttgccggtg cgagagggcg tgccagggaa
     1801 agggcggtca tgttgcctcc gaggaatcgt gctttctttt tatgcgtgca ggaaacctat
     1861 cggcgaggcg cgcccgtttc ttgacagcct tgctggaaat tgggatagtg ctatgccgcg
     1921 ttcctgacgg aaggcgaaac tgatccccgt gcttcactca ggcgatgttc gccggtcgtg
     1981 aatgccttga aggagattaa acgatgtcgc atgaaatctg gagcgagctg caggaagcgc
     2041 tcgaagcccg tgagcccttt gtattctgcg tggtgacaga gcgcacggga agcagcccgg
     2101 gcaagccctc gcagaaaatg ctcgtctatg acgatggctc gacctgtggc acggtgggcg
     2161 gaggagtgaa cgaagagcgg gtgcgacaga aagcgatgga gctctttacg tcaggtggtt
     2221 cgactatcat gacgttcgag ttgaacaatc cgatcgatgg caaagaaccg gtttgcggtg
     2281 gttcgatgcg ggtctatctc gagctgatgt gtcgtcagcc ccgcctggtc atctttggcg
     2341 gaggtcatat tggaagggtg ctggcgaaac tcgcttccat agctggttat cgggttgcgc
     2401 tcgcggatga acgcccggca tacgcctctc ccgacacgct tcctgaggtt gcagaactgc
     2461 tgtgctgccc gtattccgag gcggtcacga aagccagaat cggcaaggac gatgcggtgg
     2521 tcatcgtgac gcccggtcat gtgcatgacg tcgaggtctt gcgtgcgatg cttggtacgc
     2581 ctgcggccta catcggcatg atcggctcgc agcgtaaagt cgaaggcata cggcgcttgc
     2641 tgctcgaaga aggttttgtg tctgagcgcg tcaatcaggt gttcgcgcca atcgggatca
     2701 acctgggggg agaaacccct gaagagatcg cggtgggcat tctggcgcag ctcgttgctt
     2761 tcaataacgg ccagatcatg aagtttgagg cgaaaaaaag gaccaacgtt ccctgtcccg
     2821 cgtaagtatt gtcttgcggg aacttggtcg ttcctgcaaa tagagggaaa cttccgcgaa
     2881 ctgtcctgcg taggtataat gaaggtggaa attcaaccgt gttgtacgga ggaggcttca
     2941 tttatgagaa aacgagcgtg ctggctgctg atcatgacct ttgtgctgag tctggtcgtg
     3001 acccctcagg tgacctttgc ggccgggagc ttccttgaca ccttgcatca gacgcaggaa
     3061 ggtcaggcgg aagaccaggg tctggcacca tccgaagagt ctacatcggc agcggctgaa
     3121 ccggaacccg ccccggcaga gcctgctccc gaaagcggac aactggcatt caatcagggt
     3181 gtcagcatcg aacaattgat cgctcagatc attcagcaga tctacaatct tcttggctgt
     3241 cgcggtgtgc cgattcgcat tatcatcatt cgcagcggag gttgcacgag gcctccgacg
     3301 agtggtacgc aaccgccggt gacaacccag cctccaacga ccacgactcc tccggtcact
     3361 accacgccga ccaccccccc ccagggaacg accgatgggc agggagtagc cggtctgaag
     3421 gccgagatgg cagcaaaata cggcatcaat gccgttgacg gcgatggtgc cgtctggagc
     3481 cagcgccagc tcgaggaagc gaacaaggtt ctttccaccc ttcccgcggg tttccgtggc
     3541 tgcaccaagt ccattcagcg tgattcgatg ttccacagcc cgaatgtgct gggctacgtg
     3601 cgcatgggca ttccgacggt tcacatgctg aacagctcct gtcgcgaagg gacgttccag
     3661 ggaacgatgg tgcatgaaat gacgcatacc ttccaggcca atcatccgga tatcacccga
     3721 ttgtggcagc agacgttctg gcccaatggc cgaaatccgt ccccgccttc tgtctcatca
     3781 tatggaaaca ctcaaccggt cgaagatatg gcggaagcgg ttcgtgccta ttggcagtcg
     3841 ggctcgcgga tgaagcagac gcatcctgac cgctatgaat tcgtgaaaaa gtacatcatg
     3901 gaaggcaagg aattctgatc gcgtaagccg ctcgagagcg cttttcagac agcaggggcc
     3961 gaaaaacccc tgctgttttc attcacttac ggaatacacg tttggggagt ataatggaag
     4021 tgaacgggaa tctcggggta ccgaggaggg tgtgcgcatg agcaaaaagt tgtcattgca
     4081 tggtgtgtta ctgctgctgt tggtgctttt ggcttgtcgc tctgggcctg tctggtcggc
     4141 tgaaaacttt cttgagacga tcaattcagt cgaaaagcag cacggggtag cgattgagag
     4201 accgttgaag acccctgctc cagacagtgg cgacccctat cgcattttcg atgatggagt
     4261 cgcgtatgcg aaggctgcct cggccacgaa tagtgtcgac gcctggaaga agaagattct
     4321 gtcgaagttc ggtatcaagg cgaacgacag tgccacggcg aagtggagcg cagcgcagtt
     4381 ggaaaagctg ttcagcattc tcgacaaact gccggcaagt tatcgtcagc acacgaaaag
     4441 ccttgttcgc gtgaagatgt acaagtcaaa gtatgttctt ggatatgtga aaaggggcat
     4501 tccgtcggtc aatatcacga acgcaggttg tacgaactcg cagtttacgg ataccattgt
     4561 tcatgagatg atgcatgtgt ttctgttcaa gaataaagac aaattgtact cttggacttc
     4621 caagttctgg ccgaacggcg cgagtggaaa accgtatccc tcttccgtga cctcgtatgg
     4681 caacaccaat ccggaagaag atctctgcga atccgtcgcg ttctattttt cgaaggcgaa
     4741 gtccatgaag gcagcacagc ccgcccgtta tgaattcatc aagaagttcg tgttcttcgg
     4801 aaaggaattt tgacgtgatc aggctctgtc tggttctagt cagttgttgt gttcttgggt
     4861 ttctggccgt tcccggattc gggatgattg acaccctccc tctcccagat ctcgtacgtc
     4921 aggctgaaat gatctgcatc gcgaaggtcg ttcaggtgaa tgaagtctct gtggataaag
     4981 atcaggtctc gaccgtgaag aacgtattgc tatctgaaaa gctcttgaaa ggttcatggc
     5041 aggcgatgga accgattgtc gtgatgactc gtcagagcgg aaagtatggc accgtcggtt
     5101 cactcgaaga tcaggtggaa ttccccccca agggttccca cgtggttctg ttcctgaaaa
     5161 aggcaaaaga cggaagtctc gaacccgtca atcttctgca gggggtttgg ccgctcgacg
     5221 gccagaaacc tcttggcatg gggctgggga tcacccttca acagctcgaa gaagtggtga
     5281 aactgaaccg gtgagagaca ggctcgataa agacggcccc cagggttctg cccgggggcg
     5341 tttttatttc tggtaaaagc ggaatacttc ggatttggcc gtgactgcct gaagatgccc
     5401 ccacccttgc tcttgcgtgt gtggcgggtg agcgcgcaaa aagagcgcgg aggtgagttt
     5461 tacctgcggg cttctcgaac aaaatgaggg agttggggga tcaggatgcg gtcgacggcc
     5521 agcgtgaccg cttttgtcga gccgggcaga cagacgagaa tgcgccctga agcgagacc
//
LOCUS       DAORHD010000120         5566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14918_length_5566_cov_3.155144, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000120 DAORHD010000000
VERSION     DAORHD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14918_length_5566_cov_3.155144"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKO06_03800"
     CDS             <1..422
                     /locus_tag="PKO06_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68796.1"
                     /translation="ALSLLVCCLVIGHRTSPVLDQISTLLFAEVSEMNSLYEPIESRI
                     PPDASVSAPHHLAARLCTRRRLWFFPQGADEADYVIIPHMPLSFPAVTVAEAQQCQAR
                     LMQSGRYEQMGDQEFFTVLRRRPAHPDAGAANRKGTP"
     gene            419..1246
                     /locus_tag="PKO06_03805"
     CDS             419..1246
                     /locus_tag="PKO06_03805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV68797.1"
                     /translation="MKQHQAMHEQQFHDGWARDMRPEDILVQETMEAATQPENRYLLR
                     QLGDLRDRRILDLGSGAGETAIYLALKGARVTASDLSAGMLDLLERRAAIEGAQVTCV
                     QMSAERLPFADATFDIVYASNVLHHVPLEATLLEVRRVLKPGGVLASWDPVAYNPLIN
                     VYRRLATKVRTSDEHPFRVRDYHVCKRIFPEATFECFWFLALMIFIKMFAIDRLNPNQ
                     VRYWKYIVSEHRRLEALFLRLDRIDRLLCRVFPLIRWFCYNIAILAPKTVTSRKIAS"
     gene            complement(1307..3568)
                     /locus_tag="PKO06_03810"
     CDS             complement(1307..3568)
                     /locus_tag="PKO06_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV68798.1"
                     /translation="MKFTCQRALFVLLFVSTLTLVGAATDATQAYRAYSAAYQAYQNA
                     ISRQAPAAEVEKALHSYQTAKEAYIQSLPSAGTTTSSSASTADSSAAVSDTAVAGIPE
                     TPTGQATSGFRDDDPATFSANIQQWMNALRASPSQQYAEAIVSQITTYLAANPNLPDR
                     SRIKYELAKACDEVLGNRTLANRLYQEVADDPDSGRWGLYAKLRVKYNNALGDRLSWK
                     KSVNDKAAAMNEAFQKYKSTSWLALPVKIFRWSKYLASSVSFIRAQNKESDFQLWFEE
                     LGAPFAPTVDQVFDQYQSVNDETGLVSDNQASIKLLYNNYQSWYARWKFLSEARESID
                     IQYFIIENDIFGFSFLGHLLQKARQGVKVRLMVDARGTTKFATPLWARDYLRVMLRHP
                     NIEIKVYNPIQSNLVSMFTDIRRIVSSNHDKIVVVDGRYAIIGGRNIADEYLVDPIDD
                     PAAWLDCDVIIDSEAVAGQLDQAFVEEFNLLKAYDVGKTDAAESKLRLLDAAYTTMQA
                     ALNRRPLLDAAKVTRDIASQVKQFNEKLTQYKNMTGYPDYNPFSDAHQCPVKILDKHS
                     LSGPRNDITEAIIRFIDGSKKEIIIQNPYIVLTPRAEAALKRAARRGVPIYIHTNSPQ
                     TSDSFPTEGIIVREWRTMLKNMPTCRMFARVNAGQLHAKNFVFDGQISIIGTYNFDFL
                     SEKVNSEVVAVIKSPEFAREVRRDVMAAIAKSKEYRLATADTPEFGPEDIGSKKMWLV
                     KLCSKLGFLKPLF"
     gene            3683..5464
                     /locus_tag="PKO06_03815"
     CDS             3683..5464
                     /locus_tag="PKO06_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68799.1"
                     /translation="MEKREWQWVFEGRARMGVGLSEDGKMLRWWSVRPGHPSQKTEET
                     GEREQTVEDFRSHGPSRDNVTEWELDNPDWINHMYDPLYDGGEPGVINGPRRHEIPDA
                     VLEELAKCVGADPKLWPPPPDTTIGGEVQPTESPGTAATTPSEPANHASDPMVSRVAA
                     SLPTSRTQERAEAPVVLQQLLEKVPVGIRHWKTGKKGKKVVWTPPYRPVPPVAGEQDH
                     LNRLFRANGGLARSVRGAIFHLHHFGSLVENRLMELFGSTLAGVLFSANDLRDLIAVI
                     ADARQAARQCGTQGMPVESDVIWRFAAAKVDFSDLVREMFQRASALGKRLIRLSRLGM
                     RLLCMEDDFFRALVSEYAAGLEQLVQTDLDAEKLVGLLAKTTQRSGVHAEALLQVLPK
                     TKKALGMKTNLEVFAFYETLIKRGTYLFGMNEPSWLAEMRQKRENPNGSRNSDKVLAA
                     LNEHQELYEFAKPWLTSPRAGNQIVKAVEQMYRHHFGYSDLQADMEGQIPVVMKICHS
                     LVTSLEEFVSLCTAIPRLIEQLPRDGESTPLGWTHDFSVSDLEHRVRYCQTLEQLLSD
                     VPTSELERMNKSSQSVRTKVGKKKARA"
BASE COUNT         1173 a         1454 c         1699 g         1240 t
ORIGIN      
        1 ttgctctctc attgctggtc tgctgcctcg tgatcggaca tcgtacaagt cctgtgcttg
       61 accagatttc gacactgctg tttgccgaag tgtccgagat gaactccttg tatgagccga
      121 tagaaagccg gattccaccc gatgcttccg tatcggctcc gcatcatctg gctgcccgac
      181 tctgtacgcg tcgtcgcctc tggttctttc cccagggtgc tgatgaggcc gactatgtca
      241 tcattcctca catgccgctg tcgtttcctg cggtaacggt tgccgaggcc cagcagtgtc
      301 aggctcgact gatgcagagc ggcagatatg agcagatggg ggatcaggaa ttcttcacgg
      361 tgctgcgacg tcgtccggct caccccgatg cgggggccgc gaacaggaaa ggaactccat
      421 gaaacagcat caggccatgc atgaacagca gtttcacgat ggctgggcac gtgacatgcg
      481 tccggaagac attctggtgc aggaaaccat ggaagccgcg acacagccgg aaaaccgcta
      541 cctgttgcgc caacttggtg atctcaggga caggcggatt cttgatcttg ggtcaggagc
      601 cggagaaacg gccatctatc tggctctgaa aggagcacgg gtgacggcca gtgatctctc
      661 agccgggatg ctggatctgc tggaacgccg tgccgccatt gaaggcgcgc aagtgacgtg
      721 cgtacagatg tccgccgagc gtcttccttt tgccgacgca acgttcgaca tcgtgtatgc
      781 cagcaacgtg ttgcatcatg tgccgctcga agcgaccctg ctcgaggtga ggcgggtgtt
      841 gaagcccggt ggggtgctgg cttcctggga cccggtggcc tacaacccgt tgatcaatgt
      901 gtatcggcgg ttggcgacga aggtgcgaac gtctgatgaa cacccatttc gagtgcgaga
      961 ctatcatgtc tgcaaacgca tttttccgga agcaacgttc gagtgtttct ggtttttggc
     1021 tctcatgatc ttcatcaaga tgtttgccat cgatcggttg aaccccaatc aggtccgtta
     1081 ctggaaatac attgtttccg agcatcgtcg cctcgaggca ctcttcctca ggcttgaccg
     1141 aatcgatcgg ctgttgtgcc gagtgttccc gctgatcagg tggttctgct acaacattgc
     1201 cattctggct ccgaaaaccg tgacaagccg gaaaatcgct tcgtagccaa gctctatggg
     1261 cacagccaga ctcgctggcc atgccggtgt tgagcgtgag gaaggcttag aatagtggtt
     1321 tgagaaagcc cagcttggaa cagagcttga ccagccacat cttttttgaa ccgatgtctt
     1381 ccggcccgaa ttctggggtg tcggcggtag ccaaccggta ctcttttgat ttggcaatcg
     1441 ccgccatgac gtcgcggcga acttcgcggg cgaattcggg agacttgatg acggccacga
     1501 cttccgagtt caccttctcg ctgagaaagt cgaagttgta cgtgccgatg atactgatct
     1561 gaccgtcgaa gacgaagttc ttggcatgca gctgtccggc gttgacccgg gcgaacatgc
     1621 ggcaggtcgg catgttcttg agcatcgtgc gccactcgcg cacgatgatc ccttcggtgg
     1681 ggaagctgtc agaagtctga gggctgttcg tgtgaatgta gatgggcact ccgcgtcgcg
     1741 ccgcgcgttt gagcgctgct tcggcccgtg gggtcagaac gatgtacggg ttctgaatga
     1801 tgatttcctt cttgctgcca tcgatgaaac gaatgatggc ttcggtgatg tcgttgcgcg
     1861 gtcctgagag tgaatgcttg tcgagaatct tgaccggaca ttggtgcgcg tcgctgaacg
     1921 gattgtagtc gggataccca gtcatgttct tgtactgtgt cagtttctcg ttgaactgtt
     1981 tcacctggct ggcaatgtca cgcgtcactt tcgcggcatc aagcaggggg cgacggttca
     2041 atgccgcctg catggtcgtg taggcggcgt cgagcagccg cagcttgctc tcggcggcgt
     2101 cggttttgcc gacgtcgtag gctttcagca gattgaattc ttccacgaaa gcctgatcaa
     2161 gttgaccggc gacagcctcg ctgtcgatga tgacgtcaca gtcgagccac gcggccgggt
     2221 catcgatcgg atcaacgaga tactcgtcgg cgatgttgcg tccgccgatg atggcataac
     2281 ggccgtcgac gaccacgatc ttgtcatggt tgctcgacac gatgcgccga atgtcggtaa
     2341 acatcgaaac caggttgctc tgaatcgggt tgtagacttt gatttcgatg ttcgggtgtc
     2401 gcagcatgac ccgcaggtag tcacgtgccc acagtggggt ggcgaacttg gtggtgccgc
     2461 gggcgtcgac catcagacga actttcacgc cctgccgcgc tttctgcagc agatggccca
     2521 ggaacgagaa gccgaatatg tcgttttcga tgatgaaata ctgaatatca atgctttcac
     2581 gcgcttcgct caggaacttc caacgggcat accaggattg gtagttgttg tagagcagct
     2641 tgattgaggc ctgattgtca gacaccaagc cagtttcatc atttaccgac tggtactgat
     2701 caaacacctg gtcaacggtc ggagcgaaag gagctccgag ctcctcaaac cacagctgga
     2761 aatcactttc cttgttctgg gctcgaatga acgataccga gctcgccaga tacttgctcc
     2821 agcgaaagat tttgaccggc aaggccagcc atgaagtgga tttgtacttt tgaaaagctt
     2881 cattcatggc cgcagctttg tcgttcacgg acttcttcca gctgaggcgg tcacccaggg
     2941 cattattgta tttgacccgc aacttggcgt agagccccca gcgaccggaa tccgggtcgt
     3001 cggcaacttc ctgatacagg cgattggcga gggtgcgatt gcccagaact tcatcgcagg
     3061 ctttggccag ctcatacttg attcgactgc ggtcaggaag attgggattg gcggccagat
     3121 acgtcgtgat ctggctgacg atggcttcgg catactgctg cgaaggactg gctctcaggg
     3181 cattcatcca ctgctggatg ttggcggaaa acgtggctgg gtcatcatcg cgaaaccccg
     3241 aggtggcctg accggtcgga gtctcgggaa tgcccgcaac agccgtatcg gaaacggcag
     3301 cggagctgtc ggcggtcgat gccgacgagg aagtcgtggt tccggcgctg ggcaacgact
     3361 gaatataggc ctctttggcc gtctgatacg aatgcagagc tttttcgacc tcggcggctg
     3421 gagcctgccg cgaaatggcg ttctgatagg cctgatacgc ggcggaatac gcgcgatagg
     3481 cttgggtggc atcggttgca gctccgacca gggtcagagt cgatacgaac aacagaacga
     3541 acagcgctcg ttggcaggtg aatttcatcg ttttcctcgc tcgccagagg gttatgttgt
     3601 tcattatact gatggtactc ccttccgtcc atgagagggg ggagcacaac acagctcgta
     3661 cgagatcgaa aggtgagcgg agatggaaaa acgtgaatgg caatgggttt tcgagggtcg
     3721 ggcaaggatg ggggtagggt tgtcggagga tggcaagatg cttcgctggt ggtcggttcg
     3781 tcccggacac ccgagtcaga aaacggaaga aacaggcgaa cgggagcaga ccgtagagga
     3841 ttttcggagc catggcccgt cgcgggacaa tgtcaccgaa tgggagttgg acaacccgga
     3901 ctggatcaac cacatgtatg atccgctgta cgacggcggt gagcctggtg tcatcaatgg
     3961 accgcgtcgc catgaaatac ctgacgcggt tctggaggag ctggcgaagt gcgtgggagc
     4021 cgacccgaag ttgtggcctc caccaccgga tacgacgatc gggggcgaag tccagccaac
     4081 tgagtcgccc ggcacggcag caaccactcc gtcagagccg gccaaccatg catcggaccc
     4141 gatggtctca cgtgtggcag cgtcattgcc gacatcacga acccaggagc gtgcggaggc
     4201 tccagtcgtt ctgcagcaac ttctcgagaa ggttccggtc ggcattcgcc actggaaaac
     4261 gggaaaaaag gggaaaaagg ttgtctggac ccctccctac cgaccggttc cgcccgttgc
     4321 cggggaacag gaccatctga accggctgtt tcgcgcgaac ggaggtctgg cgcggtctgt
     4381 acgcggtgcc atctttcatc ttcaccactt tggctctctg gtcgagaacc ggctgatgga
     4441 gctgtttgga tcgacgctgg ccggggttct cttttcggcg aacgatctgc gtgatctgat
     4501 tgcagtcatt gccgatgccc gtcaggcggc acgccagtgc ggcacgcagg ggatgccggt
     4561 cgagagcgat gtcatctggc ggtttgcggc tgccaaagtg gacttttcgg atctggttcg
     4621 tgagatgttt cagcgggcgt cggccctggg gaagcgattg atcagacttt cgcgattggg
     4681 aatgcgtctg ctgtgcatgg aagacgactt ctttcgcgcc ctcgtctccg aatatgccgc
     4741 cggtctggag caacttgtac aaacggatct tgacgccgag aaactggttg ggcttctggc
     4801 aaagacaaca cagcgcagtg gggtgcatgc ggaggcgtta ctgcaggtgc tgccgaagac
     4861 gaagaaggcc ctgggtatga agacgaacct cgaggtgttt gcgttttatg aaaccctcat
     4921 caagagaggt acctacctgt tcggcatgaa cgaaccatcc tggttggcgg agatgagaca
     4981 gaaacgcgag aacccgaacg gctcgcgcaa ttcagacaag gttctggccg ctctgaatga
     5041 acatcaggaa ttgtacgagt tcgcgaagcc ctggttgacg tcgccgcgag caggcaatca
     5101 gatcgtgaaa gccgtcgagc agatgtatcg acatcatttt ggctacagtg atctgcaagc
     5161 cgatatggaa ggacagattc cggtagtgat gaagatctgt cactcactcg tcaccagcct
     5221 ggaagaattc gtgagcctgt gcacggccat cccgcgtctg attgaacaac tcccgcgtga
     5281 tggcgaatca acgccgctgg gttggacgca cgatttcagc gtcagtgatc tggaacaccg
     5341 ggtgcgatat tgccagacgc tggaacagtt gctttcagat gtgccaacgt ccgagctgga
     5401 aagaatgaac aaaagctctc aaagcgtacg aacgaaagta ggaaagaaaa aagcgagagc
     5461 atagcgagcc attccctggc ctggaagcca acgatatgat gtcctgtcgc gtcaggggcc
     5521 cctgagaaaa cctgctgggt ttcgtggtta cagcagcagc gtgtcg
//
LOCUS       DAORHD010000121         5550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_14990_length_5550_cov_4.605460, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000121 DAORHD010000000
VERSION     DAORHD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14990_length_5550_cov_4.605460"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKO06_03820"
     CDS             complement(<1..837)
                     /locus_tag="PKO06_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68800.1"
                     /translation="MNDWELFLRLRHSDAQAPVRWRGQVVDEAKARELLTEAVGGALA
                     PEWQEFLEPFLFSPSLSPNLLPPSQPLLHELCAVGREVAATLPASLPFIDRCLLVYLT
                     LEDPLAHADAISCLSEALHQNRTSQRLVRVFRHRGVADFLIRHGRLSPELQADWELAG
                     QVLHLYLEHSPDLPKMLELGGEHCLRRNRPAVAEFLFRHLLELEEARIKPLPLEKRAR
                     CEGGIGRSLLAQGRWPEAEPLLQRAEEHHLTLRRPGNLDLALILTELGRGVSYWQSVG
                     GYT"
     gene            complement(837..1847)
                     /locus_tag="PKO06_03825"
     CDS             complement(837..1847)
                     /locus_tag="PKO06_03825"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNV68801.1"
                     /translation="MAKKKKTTDGQAPEAQGRAVDLLALLVPQGRVGPHQVELEVVTE
                     DVKPTTEVHDRPALSLNPLPSRSEPAPGRVDGSTGEPVVASRVSNTGQPDPTESLQKE
                     KAMPRKVSGGEPSSVSQAKARSAQSASAVSAGGCSDFQPPAGDSFDRIRADILKCTSC
                     ELYKTRTNIVPGEGNPSARLVFIGEAPGADEDATGRPFVGRAGQHLDKILTAAGFRRD
                     EVFICNILKDRPPDNRTPTVREMEACTPFLRRQLALIKPQIIGLLGNTAVKFVIGPEA
                     PGITKIHGQWFESILGIPCMPMYHPSYLLRNQSRTVGSPNWQMWQDIQALKKRFDELA
                     GS"
     gene            complement(1959..>5550)
                     /locus_tag="PKO06_03830"
     CDS             complement(1959..>5550)
                     /locus_tag="PKO06_03830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68802.1"
                     /translation="FPLAAFSAETSPETDLDSLGTGSEIKVEDFSIEVGAGTGESSSF
                     DLGDLDASGPAALPPTKPAMKEEPVVEATSGDDDGFLNQFLIQPRESGEKPASKAAES
                     IEMSVSFDEPVSVAAVEPDVAPFPGLDQSSEPTAEAPAGDVDDDLAGLIMGIEGKPAP
                     KPAPPKPVVRREPPPPAPTAPVEAASTAPEIEGLEGLHIPELETLPESESEATAEPTA
                     AVAARLTPPDAALPELTPEEHLEECLKRLAEAKDPDERYSVVLELKELKLAPAAATFI
                     QLLGDELKDIREVAAEYLGDAAAKDAVKPLIGCLSSGDASLKFIAARSLGNIRDDAAV
                     APLVKLLEEDNDDLRYVALESLGKIGSTTALKPVAAFLKARNHDLRFVACEALGNLRD
                     AQTVSLLLPMLKDPDFEVRLKTIESLGKIGSVAACDQLLVVLGEDNERMRLATIQALG
                     QIKNANAVDALIDVFGPGTPQIKEKIIWALGEIGSERAVEPLLGLSQHFTQKLIHLAL
                     EAFAKIKSAKASRYVLGILDKKDQALRLKAIEALGEIAEKATAGNMIPFLEGPEPEVK
                     MAAAKALGKIGNPIAIDPLVGRLTDSERDVRLQAIEALGLIKGAKAIPPLVNALHEQD
                     SQIITKAEWALCEMQDLAVEPLNKALLSETPEVIPSIVRVLGKIGSIRSIFPLLKVLE
                     TAEAGLKPVIADSLLAIDNHLTDGNPISVILKEGYAWAQFSMAEALATMGDERAFPLM
                     IKIARETLNDKDLKKLAGIPDKRILECSSQILFLIRMNVARLFAQVGNDKAIPVIMKY
                     FSETDNPQRQWCVEALGGIQTDGALDALIDILKRPEYQIPLELLSKQLIANKSRKLVE
                     KLVLSASHPSDVVRMAIAVVLGETRDPRAIRTLSQLVKDSADKVRSASIEAIGKIGTT
                     AAVAPAIEALRDSIEAVRAKAAQVLGELRDTAAVEFLERTTKDSSELVRRIAVKSLAA
                     MADARVPDILIQSLTDKAETVRCAAVESLGTRKDRVAIPHLIKTLEDAAEAVREKTAS
                     ALALIGDTQAIMPLLERLDDPSPLVPLACSEAIVSFKTRSYVVLVEALKHNEERIRRH
                     ACDLLVRIGEDTLVQKLLRLVMDRNNFLRENIARILGRIGDGRVVDALVQMLADRASS
                     VRRTAAEALGSLRDIRALVALKKAERDQSREVRQAASTAVQEILKANKLN"
BASE COUNT         1079 a         1677 c         1686 g         1108 t
ORIGIN      
        1 ggtgtagccg cccaccgatt gccagtagct cacgccgcgc cccagttcgg tcagaatcag
       61 agccagatcg aggttgccgg ggcggcgcaa cgtcagatgg tgttcttcag cccgttgcag
      121 caaaggttcg gcttcgggcc accgcccctg cgcgagcaga ctgcggccga tgccgccctc
      181 acagcgtgcc cgtttttcga ggggtagtgg cttgattcgc gcttcctcaa gctcgagcag
      241 atgacgaaac agaaactcgg cgacggcggg gcggttccgt cgcagacagt gttctccgcc
      301 caattccagc attttaggaa gatcggggct gtgttccaga tacagatgca gcacctgccc
      361 ggccaattcc cagtccgcct gcagctcggg ggacagacgc ccatggcgaa tcagaaagtc
      421 ggcaacacca cgatggcgaa agacccgtac gagtcgttgc gaagtgcgat tctgatgcag
      481 agcttcggac aaacaggata ttgcatcagc atgggcgagc ggatcttcaa gagtcagata
      541 caccagcaaa catcgatcga taaacggcag agacgctggc agggtggctg ccacttcgcg
      601 gccaacggcg caaagctcat gcaacagcgg ctgtgaaggc ggcagcagat tcggggacag
      661 actcggagaa aagagaaacg gctcgagaaa ttcctgccat tccggtgcca gggctccacc
      721 caccgcctcg gtcagcagtt cacgggcttt cgcctcatcg acgacctgac cgcgccagcg
      781 cacgggagcc tgagcgtccg agtggcgcag acgaagaaac agttcccaat cattcatcaa
      841 gaaccagcaa gttcgtcaaa gcgttttttc aacgcctgaa tatcctgcca catctgccag
      901 ttcgggctgc ccaccgtgcg ggattgattc cgcaacagat aggacggatg atacatcggc
      961 atgcagggaa tgccgaggat cgactcaaac cattggccgt gaatcttcgt gattccgggc
     1021 gcttcagggc caatgacgaa cttgactgcg gtgttgccga gcaggccgat gatctgaggt
     1081 ttgatgagtg ccagctgacg acggaggaac ggcgtgcagg cttccatttc tctgaccgtc
     1141 ggcgtgcggt tgtcgggcgg acggtccttg agaatgttgc agatgaacac ctcgtcgcgt
     1201 ctgaacccgg cggcagtcaa aatcttgtcg agatgctgac cggcacgacc gacgaagggc
     1261 cgacccgtcg catcttcgtc ggctccgggt gcctcaccga tgaacaccag ccgagcgctc
     1321 gggtttccct cacccggaac gatgttcgtg cgtgtcttgt agagttcgca actcgtacac
     1381 ttgagaatgt cggcgcgaat acgatcgaat gaatctcccg cgggaggctg gaaatcgctg
     1441 cacccaccgg cagatacagc tgacgctgac tgggccgagc gcgccttcgc ctgagatacg
     1501 gacgagggtt caccacccga aactttacgg ggcatggcct tctccttctg cagagattcc
     1561 gttgggtctg gctggccggt gttcgagact cgcgaggcga cgaccggttc accggtagag
     1621 ccgtcgaccc gcccaggagc aggttccgat ctcgatggca gcggattcaa cgacaaggca
     1681 ggtcgatcgt gcacttcggt tgtcggcttc acgtcttcag tcacgacttc gagttctacc
     1741 tgatgcggcc cgacacgacc ctgaggtacc aggagcgcga gcagatcaac cgcacgcccc
     1801 tgggcttccg gtgcctgccc atccgtcgtt tttttctttt tcgccacctt acaggttcct
     1861 ttttcggaca agcctgaaga cgacagcctc tgcaaagagc ttgcagaggc tgtcatgcat
     1921 gtgtttcacc ggtactcagc aagatcaccc atcggtgatc agttgagctt attggccttg
     1981 aggatttcct gcacggcggt cgaagcggcc tggcgaactt cgcgactctg atcacgttcg
     2041 gctttcttca gggcgacgag agcgcgaatg tcacgcagac tgccaagtgc ttcagccgcg
     2101 gtacgacgca ccgacgaggc acgatcggcc agcatctgca ccagcgcgtc gacaaccctg
     2161 ccgtcgccga tgcgacccag aatgcgcgcg atgttttccc gcaggaagtt gttgcggtcc
     2221 atgaccagac gcagcagctt ctgcaccagg gtgtcttcgc cgatacgcac cagcagatcg
     2281 caggcatggc ggcgaatacg ttcttcgttg tgtttgagcg cttcaaccag caccacgtag
     2341 gagcgggtct tgaagctgac aatcgcctcg gagcaggcca gcggcaccag cggcgacggg
     2401 tcgtcgagcc gttcgagcag cggcatgatg gcctgtgtat cgccaatgag cgccagagcc
     2461 gacgccgtct tttcgcgcac ggcttcggcc gcgtcttcca gcgttttgat cagatgcgga
     2521 atggccacgc gatccttgcg ggtgcccagc gactcgaccg ccgcgcaacg cacggtttcg
     2581 gctttgtcgg tcagcgactg aatcaggatg tcgggcacgc gggcgtcagc catggcagcc
     2641 agcgacttga ccgcaatgcg tcgcaccagt tcggaagagt ctttggtcgt ccgttcgagg
     2701 aactcgacgg ccgcggtgtc gcgcagctcg cccagcacct gagcggcctt ggcccgcaca
     2761 gcttcaatcg agtcacgcag cgcttcgata gccggcgcca cggcagcggt cgtgccgatc
     2821 ttgccgatcg cctcgatgga agcggaacgc actttgtcgg ccgagtcttt gaccagctgc
     2881 gacagggtgc gaatcgcacg cgggtcgcgg gtttcaccca acaccacggc gatggccatg
     2941 cgcaccacat ctgaggggtg cgacgccgac agcacaagct tctcgacgag cttgcggctc
     3001 ttgttggcga tcagctgttt cgacagcagt tcgagcggaa tctgatattc aggccgcttg
     3061 agaatatcga tgagcgcatc gagtgcgcca tcggtctgaa tgccgcccaa cgcttcaacg
     3121 caccactggc gctgcgggtt gtcggtctcg ctgaaatact tcatgatgac cggaatcgcc
     3181 ttatcgttgc cgacctgagc aaacaggcgg gccacattca tgcgaatcag aaacagaatc
     3241 tggctgctgc attccagaat gcgtttgtcc ggaataccgg ccagtttctt gagatccttg
     3301 tcgttgaggg tttcgcgggc aatcttgatc atgagcggga acgcgcgttc atcgcccatc
     3361 gtcgccaggg cttcggccat cgagaactgc gcccaggcat agccttcctt caagatgacc
     3421 gagatcggat tcccatcggt cagatggttg tcgatcgcca gcagactgtc ggcaatcacc
     3481 ggcttgaggc cggcctcagc cgtttcgagc actttcaaca gggggaagat ggagcgaatg
     3541 ctgccgattt tgccgagcac gcgcacgatc gagggaatga cttcgggggt ctcagagagc
     3601 agcgccttgt tcagcggctc gaccgcgaga tcctgcatct cgcagagcgc ccattcggcc
     3661 ttcgtgatga tctgggaatc ctgctcatgc agggcgttca ccagcggcgg aatcgccttg
     3721 gcgcccttga tcagccccag cgcttcgatg gcctgcagac gcacgtcgcg ttctgaatcg
     3781 gtcaaccgtc cgaccagcgg gtcgatggca atcggattgc cgatcttgcc gagggctttg
     3841 gcggccgcca tcttgacttc gggttccggg ccttcgagga acggaatcat gttgccggcc
     3901 gtggccttct cggcgatctc gccgagtgct tcgatcgcct tcaggcgcag ggcctggtct
     3961 ttcttgtcaa gaatgccgag aacgtaccgg ctggctttcg ctgatttgat cttggcaaaa
     4021 gcttcgagag ccaggtgaat cagtttttgg gtgaaatgct gcgacagccc gagtaacggc
     4081 tcgaccgcgc gctcgctgcc gatttcacca agcgcccaga tgatcttttc cttgatctgg
     4141 ggagtgccgg ggccgaagac atcgatcaac gcatcaacgg cgttggcgtt cttaatctgc
     4201 cccagggcct gaatggtcgc cagtcgcatg cgttcattgt cctcacccag cacgaccagc
     4261 agctgatcgc aggccgcgac agacccgatc ttgccgagcg actcaatggt cttgaggcgc
     4321 acctcgaagt cggggtcttt cagcatcggc agcagcagac tgaccgtctg agcatcgcgc
     4381 aggttgccca gagcttcgca ggccacgaag cgcagatcgt gattgcgagc cttcagaaag
     4441 gcggcaacag gcttgagagc ggtggtcgac ccaatttttc ccagactttc gagcgccaca
     4501 tagcgcagat cgtcgttgtc ttcttcgagc agcttcacga gcggggcgac tgccgcatca
     4561 tcgcgaatat tcccgagcga gcgggccgca atgaacttca gtgaggcatc accggaactg
     4621 agacagccga tcagcggctt gaccgcatct ttggccgcgg catcgccgag atactcggcg
     4681 gccacttcgc gaatgtcctt cagctcgtcg cccagcagtt gaataaaggt cgccgccgcg
     4741 ggggccagct tcagctcctt cagttccaag accacggagt agcgctcatc ggggtccttg
     4801 gcttccgcca gccgtttgag acactcttcg agatgttctt cgggcgtgag ttcgggcaga
     4861 gccgcatctg gcggtgtcag acgtgccgca acggcagccg tcggttccgc agtggcttca
     4921 gactcgctct ccgggagggt ctccagttca ggaatgtgca gcccttcaag accctcgatc
     4981 tccggagcgg ttgacgcggc ctcgacgggt gcggtcggtg caggcggcgg gggttcgcgg
     5041 cggacaaccg gcttcggcgg cgcgggtttg ggcgctggct tgccctcgat gcccatgatc
     5101 aacccggcca gatcgtcgtc gacatcgccg gcaggcgctt cggcagtcgg ttcagagctc
     5161 tgatcaagcc cgggaaaggg cgcgacgtca ggttcgacgg ccgcaacgga aaccggttca
     5221 tcgaaagaca cgctcatctc gatcgattcc gcagccttgg atgcgggttt ttcaccagac
     5281 tccctgggct gaatcagaaa ctgattcagg aacccatcat catcgccaga agtggcttcg
     5341 accacgggtt cttctttcat cgccggcttg gtcggcggca gtgcggctgg tcctgacgca
     5401 tcgagatcac ccagatcgaa ggaactcgat tcgccggttc cggccccgac ttcgatcgag
     5461 aaatcttcaa ctttgatctc agagcccgtg cccagcgagt cgagatctgt ttccgggctc
     5521 gtctcggcag aaaaggcggc aaggggaaag
//
LOCUS       DAORHD010000122         5536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15035_length_5536_cov_3.635103, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000122 DAORHD010000000
VERSION     DAORHD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15035_length_5536_cov_3.635103"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3195
                     /locus_tag="PKO06_03835"
     CDS             <1..3195
                     /locus_tag="PKO06_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68803.1"
                     /translation="LWQVFGTLETHHEIKAKARTRQRLQQEMAVVERSHNPLFHMARW
                     LRLDSERVLERAERQGKTPQECLETLEKHLVRQQARTAHVQWILVKDPPPPVLLRVTF
                     RELGGSTCTISYPLSAEIFRAIGEDRFRMLDLRMQELFRRIQNSCHRVGFFPRAFWQA
                     LLTKHPSVICSWEQRVRQPVASGDRDILRGPEIHVLHRMFLRKMGFRDPMYRDQLRQD
                     AETVISQVFPGWMRFDKLDYLFECSCAQFWPVKMHLRERLMCWFPLFEPRWLNDLSRL
                     PPKTQGMEECETQVEQHLWGSLQFYHDPDVDRTRESLRCLMRLLATRGVCVAVLHPRE
                     DNRILMKSRAWRNGLPYDPSVLQPAQIRDIDAWMVVRTRIVLGQGFPLLLGMSWKDEQ
                     FRRETRARGFLLVGSLTAIFGLLLFLIRPFYVSLPYQLSVLFALVMAPPVIQGVITID
                     QLSVERQFRMYADARRELERSMDGLDKSFELGLAWPIAFVRATVASAAQNPDWLKPVP
                     AGSPDSGFEQAMADLPWYGLTLNLAAKHVAGQLPRMAQLFVSRAEGEEYQLMGAFGIV
                     IGSLFRQLGFEDVANKTAHQSTKDLGEGFKASEIVALLQRLLFAESFANLFFSIESLV
                     DMSVFGRQNYFYNRFGGLQNHPWLVQTVFTEGHLKGPIFRDWMRRWGLVSVWNGRTDQ
                     PLFQRFPPFVILFAISEKPQLIECVEPAPTLLTHLSQLSTLTGLWLFDSIGLGSETRM
                     LGVGPSRTMPRNLLYAQYRIGEEQLQESRYWHEVQRLLYLLITLPFLLALLIGRRLVE
                     PLIELRETASRVASGDYAARLNPHRGGEFAVLARVFRDMTESLAGGKLLQLFVSESVR
                     QSVADGSGTFHAKPREEYRLVLFVSLSGLRQHWKQMPATQILAELNVFLEAASREIRL
                     AGGDVDKFVGEKVLAAFSARNGETREAMLVRAAGVVRTILRWGSVRTEWKAFPPSIGM
                     IWGPLLVGRLGTEDVRLEQAMIGDTVNLASRLCDQALNAGGGCLTDDRTATLLASLLE
                     RIPDLKLERLGSARIKGKRREVEVCQLTCGEKTA"
     gene            3192..>5536
                     /locus_tag="PKO06_03840"
     CDS             3192..>5536
                     /locus_tag="PKO06_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV68804.1"
                     /translation="MSRSLFPSSLVQPLIVSLLPLFLLIWNLDTDWKKAVVHQQEVFR
                     EMTNRLQVRVRRLTSPEFWPEHWTRRLSTRLAQTIEQHSSTEHIEKLVQTWKTQVASG
                     AMVWVATCASDSDRASLLLEGGTGYRKVWSDLFHFLFACFKNPIELALSREQHNLIRG
                     LFGDHFPVSLLAPEMRGRPFPVLFRGSSFWLCWELLFAEPGPASGSRKRRLLGGVIVM
                     FPQQQLQYRQVMELVLRRWRAMVPEAKGVECAFLPLPVKPGPLMEPPMGDIRWLQGVV
                     KQRMVSFLRQLELKGSTNGWIAGDPVLPGSAINDILDLETHWGRVLPAAPILGHAVML
                     CGPKRAVTEPMRAILARWVFAFWLIGWLVVFVFRWWFGRFPDPGIRLGMQLWFLGLLA
                     VPLILMVLTANNWLLDYRSNLLLAKRQEMEQVIAQIEAGTRVLNEEQTQLLNRLSTSP
                     DLGRQLKEVEEGRLASAVFMDHFLESVLQSGLDPGAIFFYGHGGFEVSIMRRAHIVDQ
                     EIEMRRFVSTLAEGLLQHADPELAREYRLREERRNVAGAKKANPFQGLSSNPMTYLQI
                     GSTEEFRMGRVSMMKFHSSIRWQGRVHYCLFVFWSFDRACRVFLKSQIPKMNIAYRDL
                     GIAAYRELQDGVEVIFEPRVDESMQRWAELAREQPVFREQDGMLVLAVQSRTLPGYLL
                     VVRGSLDGILQAVQTERFRLLMAALLLAFMVALSGYGLSTWLSDPILQMTLAIQKIAG
                     GNLDIRVTEPRPDELGSAGESIDQMTRQLQERRRMSRFVAP"
BASE COUNT         1092 a         1424 c         1684 g         1336 t
ORIGIN      
        1 ctgtggcagg tgttcgggac actcgagact caccacgaga tcaaggcaaa ggccaggacc
       61 cgtcagcggt tgcagcagga aatggcggta gtggaacgat cgcacaatcc actgttccat
      121 atggctcgct ggctgaggct cgacagtgaa cgagtattag agagggcgga acgccaggga
      181 aagacaccgc aggagtgtct ggagacactt gagaagcatc tggtgcgcca acaggctcgc
      241 actgcacatg tgcaatggat tctggtaaag gatcccccac ctccggtgct gcttcgcgtg
      301 acttttcgtg agcttggcgg aagcacgtgt accatttcgt atcctctttc ggccgagatt
      361 ttccgcgcta tcggtgaaga ccggttccga atgctggatc tccgcatgca ggaactgttt
      421 cgtcgtatcc agaattcctg ccatcgcgtc ggattttttc ccagggcttt ctggcaggcc
      481 ctcctgacga aacacccgag tgtgatctgt tcctgggaac agagggttcg acaaccggtg
      541 gcttccggag accgcgacat tctgcgtggt ccggagatac acgttctgca tcgcatgttt
      601 ctgcgcaaga tgggtttccg cgatccgatg tatcgcgacc agttgcgaca ggatgcggaa
      661 accgtgatta gtcaggtgtt tcccgggtgg atgcgtttcg ataagctcga ttatcttttc
      721 gagtgttcct gtgcccagtt ctggcccgtc aaaatgcatc tgcgcgaacg tctgatgtgt
      781 tggttcccgt tgtttgaacc ccgttggtta aacgatctga gtcggcttcc accaaaaaca
      841 cagggcatgg aagaatgcga aacacaggtg gaacaacatc tgtggggaag tctgcagttt
      901 taccatgacc ccgacgtcga caggacgaga gagagtcttc gttgtctgat gcgattactc
      961 gcgacgcgtg gagtatgcgt tgccgtcctt cacccaaggg aagacaatcg catcctcatg
     1021 aaaagcagag cgtggaggaa tggattgccc tatgatccgt cagtgctgca acctgctcaa
     1081 attcgtgata tcgatgcgtg gatggttgta cgaacccgaa ttgttcttgg ccagggattc
     1141 cctttgctac tcggaatgtc ctggaaagat gaacagtttc ggcgagagac gcgtgcccgg
     1201 ggttttttgc tggttggctc tttgaccgcc attttcgggt tactgctgtt tctgatcaga
     1261 ccgttttatg tctctctccc gtaccagctg tcggtgctgt ttgctctggt catggcgccg
     1321 ccggtgatac agggcgtgat cacgatcgat caattgtcgg tcgaacgaca gtttcgcatg
     1381 tatgcagatg ctcgtcgaga gcttgagcga tcgatggatg gactggacaa atcatttgaa
     1441 ttgggtttgg cgtggccgat tgccttcgtt cgtgcgactg tggcttcggc ggctcagaac
     1501 cctgactggt taaagcccgt gccagccggc tcacccgact cgggctttga acaggccatg
     1561 gccgatcttc catggtatgg actgactctg aatctggcgg cgaaacatgt cgccggccag
     1621 ttgccgcgca tggcacaact gttcgtttct cgtgcggaag gagaagagta tcagctgatg
     1681 ggggcgtttg gcattgtgat cggatcgttg ttcagacagc ttggctttga ggacgttgcc
     1741 aacaaaaccg cgcaccagag caccaaggat cttggcgagg gattcaaggc atccgaaatc
     1801 gtcgcgctcc tgcaacgcct gctgttcgcc gaatcatttg cgaatttgtt cttctccatt
     1861 gaatcgctgg tcgacatgtc ggtcttcggc aggcaaaact atttctacaa ccgctttggt
     1921 ggtctgcaga accatccctg gctggtacaa acggtgttta ccgagggaca tttgaagggc
     1981 ccgatttttc gtgactggat gaggcgttgg ggattagtca gcgtctggaa tgggcgaacg
     2041 gaccagccac tctttcagcg atttccaccc ttcgtgattc tgttcgcgat ctcagaaaag
     2101 ccgcagctca tcgagtgcgt tgaacccgct cccaccttgc tgactcatct ttcacaactc
     2161 agtaccctca cgggattatg gctgttcgat tcgatcgggc tgggtagtga aacccgtatg
     2221 ctgggcgtcg gaccctcgcg taccatgccg agaaatctgt tgtacgccca gtatcgaatc
     2281 ggcgaagagc agcttcaaga gtcgcgttac tggcatgaag tgcaacgtct tctgtatctg
     2341 ctgatcaccc tgccttttct gctcgctttg ctgattggca ggcgattggt tgaaccactg
     2401 atcgaactgc gcgagaccgc gtctcgcgtc gcctcaggag actatgcggc gcgactgaac
     2461 ccgcatcgtg gaggcgagtt tgcagtactg gccagggtgt ttcgtgacat gacggaaagt
     2521 ctggctggtg gaaaactgct gcagttgttt gtgtctgagt cggttcggca gtctgtcgcc
     2581 gatggttcag ggacgtttca tgcaaagcct cgcgaagagt atcgactcgt cctgttcgtg
     2641 tcactgtcag gtttgcgaca acattggaaa cagatgcccg cgacccagat tcttgcggag
     2701 ttgaacgtct ttctcgaagc ggcttcacgt gagattcgtc tggcgggtgg tgatgtcgac
     2761 aaatttgtcg gagaaaaggt gttggcggcc ttttccgcgc gaaatggcga aacgcgtgag
     2821 gcaatgctgg tccgcgcggc aggagtggtg cgcacaattc tgcgctgggg ctcggtgcga
     2881 actgagtgga aagcttttcc accctcaatt ggcatgatct ggggccctct gctggttggg
     2941 cgtctgggaa ccgaagatgt gcgcctcgaa caggcgatga tcggcgatac cgtgaatctc
     3001 gcctcacgct tgtgcgatca ggcgctgaat gccggtggag gatgcctgac cgatgatcgc
     3061 acggccaccc tcctggcctc actgctcgaa cggattcccg acctgaagct ggaacgtctc
     3121 ggaagcgccc ggatcaaagg caagcggcgc gaagtggaag tctgtcagct cacctgcggg
     3181 gaaaagaccg catgagtcgt tcgctgtttc cgtcgtctct ggtgcaaccc ctgattgtca
     3241 gcctgctacc gttgttcctg ctgatctgga atctggacac cgactggaag aaagcggttg
     3301 ttcaccagca ggaagtgttt cgagagatga ccaatcgtct gcaagtgcgc gttcgacgac
     3361 tcacttcacc ggaattctgg cccgaacact ggactcgacg tctcagcacc cgtctggcac
     3421 agacgatcga acagcattcc tccacagaac acatcgaaaa gttggttcag acgtggaaaa
     3481 cgcaagtcgc atcaggtgcc atggtgtggg ttgcgacgtg tgcgtcggac agtgatcggg
     3541 cgagtctgtt gctggaaggg gggaccggtt atcgcaaggt ctggtccgac ctgtttcact
     3601 tcctgttcgc ctgtttcaag aatccgatcg aacttgcact gagccgggaa cagcacaacc
     3661 tcattcgcgg gctctttggg gaccattttc ccgtcagtct gctggccccg gaaatgcgag
     3721 gtcgcccatt tccggttctc tttcgtggca gttccttctg gttgtgctgg gaactcctgt
     3781 tcgcggagcc aggaccagcc tcgggctctc ggaaaagacg attgctgggt ggagtcattg
     3841 tcatgtttcc gcaacagcag ctgcagtacc ggcaggtcat ggagctcgtg cttcgccgtt
     3901 ggcgtgccat ggtcccggaa gccaagggag tcgagtgtgc gtttctcccg ctgccagtga
     3961 aacccggacc actcatggaa ccccccatgg gagatatccg ctggctgcaa ggggtggtga
     4021 agcagcgcat ggtcagcttc cttcgccaac tggagctgaa gggttcgacg aatggctgga
     4081 tcgctggtga tcccgttctt cccgggtcag cgatcaacga cattcttgat ctggagacgc
     4141 actggggtcg agttctcccg gcggctccga ttcttggtca tgcagtcatg ttgtgtgggc
     4201 caaaacgtgc agtaacggaa ccgatgcgcg cgatactcgc ccgatgggtg tttgcgttct
     4261 ggctgatcgg ctggctggtc gtgtttgtct ttcgatggtg gttcggccgc tttccagatc
     4321 ctggaatacg cctgggtatg cagttgtggt ttctcgggct gttggctgtg cccctgatac
     4381 tgatggttct gacggccaac aactggctct tggactatcg ttcgaatctg ctgttggcca
     4441 aacgccagga gatggaacag gtgatcgcgc agatagaagc ggggacccgg gtgttgaacg
     4501 aggaacaaac tcagctcctc aaccggttgt caacatcccc ggatctggga aggcagctga
     4561 aggaggtcga agagggcagg ctggcgtcag ccgtgttcat ggatcacttc ctggagtcgg
     4621 ttctgcagtc gggcttggat ccgggggcga tcttttttta cggacacggt gggttcgagg
     4681 tatcgatcat gaggcgggca cacatagtcg atcaggaaat cgagatgcgc cgattcgtaa
     4741 gcacgttggc agagggtctg ctgcaacacg cagatcctga gctggcacgg gaataccgtc
     4801 tacgtgagga acgtcgcaat gtcgccggag caaaaaaggc gaatcccttt caaggtttgt
     4861 cgtcaaaccc gatgacctat ctgcagattg gcagcacgga agagtttcgc atggggcggg
     4921 tcagtatgat gaagtttcac tccagtatcc gctggcaggg acgggttcac tattgtctct
     4981 ttgtcttctg gagctttgat cgcgcctgcc gggtgtttct gaaaagccag ataccgaaga
     5041 tgaacatcgc gtatcgcgat ctgggcattg cagcctatcg agagttgcag gatggggtgg
     5101 aggtgatttt cgaacctcgt gttgatgagt cgatgcaacg ttgggcggag ctggcgcgag
     5161 agcaaccggt ctttcgagaa caggatggaa tgctggttct cgcggttcag agtcggacgt
     5221 tgccgggata tctgctggtc gttcgcggct ctctggatgg cattctgcag gctgtccaga
     5281 cggaacggtt tcgcctgttg atggcggcac tgttgctggc gttcatggtg gctctgtcgg
     5341 gctatgggct ctcgacctgg ctatcggatc cgattctgca gatgacgctt gccatacaga
     5401 aaattgccgg tggcaacctg gacattcggg tgaccgaacc tcgcccggat gaactcggca
     5461 gtgctggcga gagcattgac cagatgactc gccaactgca ggaacgtcgt cgcatgagtc
     5521 gcttcgtcgc acctca
//
LOCUS       DAORHD010000123         5533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15045_length_5533_cov_4.309237, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000123 DAORHD010000000
VERSION     DAORHD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15045_length_5533_cov_4.309237"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..354)
                     /locus_tag="PKO06_03845"
     CDS             complement(55..354)
                     /locus_tag="PKO06_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNV68805.1"
                     /translation="MSESKNHDQPFRFEDIAVAVKYNFGAKGEAPTVIASGRGFLASR
                     IKTLAQEHEVPVVAEKPLAESLAQVPVGSEIPAELWEAMAAVLAHLYSLDGSLNR"
     gene            complement(351..1427)
                     /locus_tag="PKO06_03850"
     CDS             complement(351..1427)
                     /locus_tag="PKO06_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68806.1"
                     /translation="MKISLQAPAQAFTRGQTVRGEVVGHQDGQSLAVVNGVMTRIPRH
                     LPIGHTFSGNIQQNESGETVVESQASDLKNRAASMQISHPQVKLEPTPENRKLVELMK
                     QFGIPITPETLEEIKHRLPQGSGFADSAKLRAILLMLSRGLPEQQLSALEKYFSGRQH
                     LADLFARLDSSSLDQLRKQWGLGRSVTTLIELIEQSSSEGKSILSQTADQLAENLQFQ
                     SLFEMSPAESGDHQLYFQWPLFWQGQNAPDTLEGECFFARKDGQNRGFSVRVMVHPPN
                     LGNMEIGIHEFEKALWVHFGVRESSARDLLPTVFEPLRHALLDQGWKSVKLTLGIKPP
                     RSQLLEPLQTTPSEPEHPELDLKA"
     gene            complement(1424..2101)
                     /locus_tag="PKO06_03855"
     CDS             complement(1424..2101)
                     /locus_tag="PKO06_03855"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV68807.1"
                     /translation="MRNHHEMAARQAGHVLIAGGDEAGRGPWAGPVVAAMVILPESYE
                     LPGLDDSKKLSRDQRRRLFDQIRQTAIAFGVGSASVEEIDRINILEATRLAFHRAFDQ
                     LVPAPNYLLLDYIKLPWLTLPHQAFPRGESESDSIAAASILAKETRDLLMEQLDAEFP
                     GYGFRNHMGYGTPAHQKALQERGPCAIHRRSFKPIKAFTDSARVSQLDLFAESASDHS
                     SGTRRQR"
     gene            complement(2195..3394)
                     /locus_tag="PKO06_03860"
     CDS             complement(2195..3394)
                     /locus_tag="PKO06_03860"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV68808.1"
                     /translation="MKAKNRDELQALLREGLSWCMKCGACLSGCPVYRHRRTEGSSMR
                     GKLALLQAIEAGRTLPPETVAEVLSHCTGCQTCRSLCPNGIDTVFLTLVARLILAQDG
                     THDWTDRAGSSVRKYQLSSRLTLAAMPMLMHGIEGVRAGRLLRSLPRRVFHALRAEPD
                     FAAFQTGSEILFPGCALSQDPPRLQQLERVCAKVGLAVQLTPDLGCCGMPELGIASAQ
                     RHEEQVRLLFDRLQKLQPKRILVVCHRCLTGFRYLRSLYHWTAEEASLFDRIDDFTVR
                     LASLNWTATKPLAGSVTVHDPCQLLRVPGAKAATRSILARILSEPVVESSGDGTCCGF
                     GGSFLFRHPTLAGDLRRERVEQLRKTGTQRMVTACTYCADSLELPLRRQGMETSLLID
                     LLDQALS"
     gene            complement(3405..4769)
                     /locus_tag="PKO06_03865"
     CDS             complement(3405..4769)
                     /locus_tag="PKO06_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV68809.1"
                     /translation="MKQLEQFLRRLDAAIVSTDVTQTTFHAYDATNEFHYPEAVVRPR
                     DSEELHQIVLSANEYHVPLFPRGAGTGFSGGSLAVDGGVVIDFLGLNKVLDLDEDDML
                     VRVQPGVVTLHLQKLVESHKLYFPPDPASLKICTIGGNVAENAGGPHCLKYGVTRDYV
                     LAIDGFTGDGRPFAAGKAIHKNRAGYDLRHLLIGSEGTLAVFSAFTLKLIPRPETRIL
                     FLAWFRAIHDAAAMVNQLLHRGIMPSSIEFMDEGSIKAVEQHAHMGLPTGFQALLLIE
                     IDGRHDEMPLIQKRVEDCLSTSASNIQVAHQADEQEKLWEVRRKASPAMRQFGNKKAN
                     QDIVVPRKSIPTALDGLKHISQRHRINVINFGHIGDGNIHVNIMYDGNNPDDCQRAEL
                     AVHDLFDLVNSLGGSISGEHGIGLAKKKHLPRNIDTTSYDLMRAIKRDFDPHGILNPR
                     KIFL"
     gene            complement(4828..>5533)
                     /locus_tag="PKO06_03870"
     CDS             complement(4828..>5533)
                     /locus_tag="PKO06_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68810.1"
                     /translation="SKGKKLTREAGERLATIEEHTALGSGFKIAMRDLQIRGAGNMLG
                     EAQSGHIAAIGFSLYIELLEEAVKQLRGKAEARVQDAQVEIPVTALLPRQYIPDDETR
                     IEFYARLARCRDQQLLDVLRLECEDRFGSLPPEARRLMRVVSLRVKATHTGVSKITRL
                     PLHLKVEFDPARAPHLEQLFQQRSPWLRRLTFLAEEPNAVLLEARSVDADDLIDHASD
                     LLEHLRSLQPRPTPTA"
BASE COUNT         1129 a         1545 c         1694 g         1165 t
ORIGIN      
        1 agctgttctc ccaggctgcc gaggcgcttg cggccgtcgg tacctttggt tgcatcagcg
       61 gttgagcgag ccatcgagtg aatacaggtg cgccagtacc gcggccatgg cttcccacag
      121 ctcggcagga atctcgctgc cgaccggaac ctgcgccaaa gactcggcca gaggcttttc
      181 cgcgacgacc ggcacctcgt gttcctgagc caaagtcttg atacgtgagg cgagaaagcc
      241 gcggccagag gcaatgaccg ttggtgcctc tcctttggcg ccgaagttgt atttcaccgc
      301 gaccgcgatg tcttcgaatc ggaacggttg atcgtggttt tttgactcgc tcatgctttc
      361 agatccagtt ccgggtgctc aggctcggag ggcgttgttt gcagaggttc gagcagctgt
      421 gaccgggggg gcttgatgcc gagggtcagt ttgacagact tccagccctg gtcgagaagc
      481 gcgtggcgca ggggctcgaa cacggtgggg agcaggtcgc gggccgacga ctcgcgcacg
      541 ccgaaatgca cccagagagc tttttcgaac tcatgaatgc cgatttccat gttaccgagg
      601 ttgggcgggt gtaccatcac gcgaacggaa aaaccgcgat tctgaccatc ctttcgggcg
      661 aagaaacact ctccttcaag ggtgtcggga gcgttctggc cttgccagaa cagcggccac
      721 tgaaaataca gctggtggtc accgctttcc gcgggagaca tttcgaacag cgactgaaac
      781 tgcaggttct cggcgagttg gtctgcggtc tgtgacaaga tcgacttgcc ttcagaagac
      841 gactgttcaa tgagttcgat gagcgttgtc acgctgcgtc cgagtcccca ctgcttgcga
      901 agctgatcga gagaactgct gtcgaggcgt gcgaacaaat ctgcgagatg ctgcctgccg
      961 gaaaagtact tctcgagcgc cgacagttgc tgctccggaa gcccgcgcga gagcatgaga
     1021 agtatggctc gcagttttgc ggaatccgcg aatccgcttc cctgaggcaa gcgatgcttg
     1081 atttcttcga gtgtttccgg ggtgatcggt atgccaaact gtttcatcag ttcgacgagt
     1141 tttcgattct ccggtgttgg ctccagcttc acctgagggt gtgatatctg catcgatgca
     1201 gcgcgattct tcagatctga cgcctgggac tcaacgacgg tttcgccact ttcgttctgc
     1261 tgtatgttac cggaaaacgt atgtccgatc ggcagatgac ggggaattcg cgtcatgacc
     1321 ccattcacca ccgcgagcga ctgtccgtcc tggtgaccga cgacctcgcc gcgtaccgtc
     1381 tggccgcggg tgaatgcttg cgcgggggcc tgcagcgaaa tcttcatcgc tggcgacggg
     1441 tgcccgacga atggtcagaa gcgctttccg caaacagatc cagctgcgat acgcgtgcag
     1501 aatcggtgaa agccttgatg ggcttgaaag accgtcgatg aatggcacac ggtccccgct
     1561 cctgcagcgc tttctgatga gctggggtgc cataacccat atggttgcga aaaccatatc
     1621 cggggaactc agcgtccagt tgttccatca agaggtcgcg ggtttccttg gcgagaatcg
     1681 atgccgccgc aatggaatcg ctttccgact cgccgcgcgg aaaagcctga tgcggtaatg
     1741 tcagccaggg aagcttgatg tagtcgagaa gcagataatt cggggctggt acaagctggt
     1801 cgaatgctcg gtgaaaggcc aggcgtgtgg cttccagaat gttgatgcga tcgatttcct
     1861 cgacactggc gctgccgaca ccgaaggcga tggcggtttg cctgatctga tcgaagagcc
     1921 gtcgccgctg atcgcgcgac agttttttcg agtcatcgag accgggcagc tcataagact
     1981 cgggaagaat caccatcgcc gcgacgacag gtcccgccca gggcccgcga ccagcttcgt
     2041 cgcctccggc aatcaagaca tggcccgcct gtcgggccgc catttcgtga tggttccgca
     2101 tatcaacgac tacactatct cagctcacgt gaccacgcaa ctgccaagcc gtttgccctg
     2161 gcaaatgccc aggcaagcgc agggtgggaa aaagtcagct cagggcctgg tcgagcaggt
     2221 cgatcaacag gctggtctcc attccctggc gtcgtagtgg cagttcaagg gaatcggcgc
     2281 aataggtgca ggcagtgacc atgcgttggg ttccggtctt tctcagttgc tcaacccgtt
     2341 cacgtctcag gtcgcctgcc agggtcgggt gccgaaagag aaaactgccg ccgaaaccgc
     2401 agcaggtacc atctccggaa gactcgacca cgggttcaga aaggattcgc gcgagaatgg
     2461 atcgtgtcgc ggccttggcg ccaggaacgc gcagcagttg gcaggggtca tggacggtta
     2521 ctgagcccgc cagcggtttc gtggcagtcc agttcagtga tgccaggcga acggtgaagt
     2581 cgtcgatgcg gtcgaataga gacgcttctt cggcggtcca gtgatagagc gagcgcaggt
     2641 agcgaaaccc ggtgagacag cgatggcaga cgacgagaat gcgcttcggt tgaagtttct
     2701 gcaaacgatc gaacaacagg cgaacctgtt cttcatgccg ttgtgctgac gcgattccga
     2761 gctcgggcat gccgcagcag ccaagatcgg gggttaactg aacggcgaga cccactttgg
     2821 cgcagactcg ctcgagttgc tgcaatctgg gtgggtcctg gctgagagcg caaccgggaa
     2881 agagaatctc tgagccggtc tgaaacgcgg cgaagtctgg ctcggcgcgt agggcgtgaa
     2941 agacccgtcg tggcagagaa cggagcaagc gaccagcgcg aacaccttcg attccgtgca
     3001 tcaacatggg catggctgcc agtgtcaacc gactcgacaa ctggtacttt cgcaccgaag
     3061 atcccgctcg atcagtccag tcgtgtgtgc cgtcttgagc aagaatcagt cgtgccacga
     3121 gggtaagaaa cacggtatcg atgccgtttg ggcagagtga acgacaggtt tgacagccgg
     3181 tgcaatggct gagaacctcg gcaacggttt cggggggcag ggtccgccct gcttcaattg
     3241 cttgcaagag cgcgagtttg ccacgcatcg agctgccttc ggtgcggcga tgccgataga
     3301 ccgggcaccc cgacagacag gccccgcact tcatacacca ggaaagccct tcgcgtaaaa
     3361 gagcctgcag ttcgtcccgg tttttcgctt tcacgtggcg tggctcacag aaaaatcttg
     3421 cgggggttga gaatgccgtg cgggtcgaag tcccgcttga tggcccgcat cagatcatac
     3481 gaggtcgtat cgatgtttct cggcaggtgc ttcttcttgg ccaatccgat gccatgttcg
     3541 ccactgattg agccgccgag tgagttgacc agatcgaaca ggtcgtgcac cgcgagttca
     3601 gctctctgac aatcgtccgg gttattacca tcatacatga tgttgacgtg gatgttcccg
     3661 tcgccaatat gtccgaaatt gatgacgttg atgcgatgtc gctgcgagat gtgtttcaat
     3721 ccgtccaagg cggtgggaat cgatttgcga gggacgacaa tgtcctgatt ggctttcttg
     3781 ttgccgaatt gtcgcatcgc cgggctcgcc ttgcgcctga cttcccacaa cttctcctgc
     3841 tcgtcggcct ggtgagccac ctgaatgttt gaggcgcttg tactgaggca gtcttcaact
     3901 cgcttctgaa tgaggggcat ttcatcgtgt cgtccgtcaa tctcgatgag cagcaacgcc
     3961 tgaaaacctg tgggcagacc catgtgtgca tgctgctcga cggctttgat cgagccttca
     4021 tccatgaatt cgatcgaaga aggcatgatt cctcggtgca ggagttgatt gaccatcgcg
     4081 gcggcgtcgt gaatggcgcg aaaccaggcc agaaacagaa tgcgggtttc cggcctggga
     4141 atcagcttca gggtgaacgc actgaacacc gccagggtgc cttcgctgcc gatcaacaga
     4201 tgacgcaggt cgtagccggc tcggttcttg tgaatcgcct ttccagccgc aaatggccga
     4261 ccatcgcccg tgaaaccatc aatggccaag acgtagtcgc gtgtgacccc gtatttcaga
     4321 caatgcggtc ccccggcatt ctcggcgaca ttgccgccga tggtgcagat tttcagtgaa
     4381 gccggatcag gcgggaaata cagcttatgg ctttcgacca gcttctgcag gtgcagggtg
     4441 acgacgccgg gctgcacacg caccagcatg tcgtcctcgt cgaggtcgag aaccttgttc
     4501 aggcccagaa aatcaatgac gacgcccccg tcgaccgcca gggaaccgcc cgaaaaaccg
     4561 gtgcccgcgc cacgcggaaa gagcggcacg tgatactcat ttgccgacag aactatctga
     4621 tgcaactctt cgctgtctcg aggtcggaca accgcctcgg gatagtgaaa ctcgtttgtc
     4681 gcgtcatagg cgtggaaagt cgtctgcgta acgtctgtgc tgacgatggc ggcatcaaga
     4741 cgacggagaa attgttccag ttgcttcacc agaagaacct ttcgtcagac gaggtgtgcg
     4801 gcagtatagg catgtcgtga ctccgggtca agcggtcggg gtgggtcgag gctgcagcga
     4861 acgcagatgt tccaggaggt ctgaggcatg gtcgataaga tcgtcggcat cgaccgatcg
     4921 tgcttcgagc agtacggcgt tcggttcttc ggcgagaaag gtcagccggc gcaaccaggg
     4981 ggagcgctgt tgaaaaagtt gctcgaggtg cggagcccgt gcggggtcga actcgacttt
     5041 gaggtgcagg ggcaggcggg tgatcttcga aactcccgtg tgcgtcgcct tcacccgcag
     5101 actgaccacg cgcatcagac ggcgtgcctc tggtggtagt gagccaaagc ggtcttcgca
     5161 ctcgagtcgc aaaacgtcaa gcagctgctg gtcccggcac cgtgccagtc gcgcataaaa
     5221 ctcgatgcgc gtctcatcat cgggaatata ctgccgtggc agaagcgccg tcaccggaat
     5281 ttcgacctgg gcatcctgga ctctcgcttc agccttgccc cgcaactgct tgacggcttc
     5341 ctcgagcaac tcgatgtaga gcgagaaccc gattgccgcg atgtgaccac tctgggcttc
     5401 acccagcatg ttgccggcac cgcgaatctg cagatcgcgc atggcgatct tgaaacccga
     5461 tcccagggcg gtgtgttctt cgatcgtggc cagacgttcc ccggcttcac gggtcagctt
     5521 cttgcccttg gag
//
LOCUS       DAORHD010000124         5516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15133_length_5516_cov_3.304523, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000124 DAORHD010000000
VERSION     DAORHD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_5516_cov_3.304523"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="PKO06_03875"
     CDS             complement(<1..613)
                     /locus_tag="PKO06_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68811.1"
                     /translation="MRHGKLALLSVVSVVSLFLVGYRPQWREMYTKWCLANQIAIEQA
                     VQLGPASSLPPLDVEVRLEDYRNRLPENFGEIPQCPVYREVGLATHSYLLQVSASGTT
                     VVRCLLHGTRSDPVVVPSDRGLQMLLERAGELRQQRQMRTALVLALVWCFGPWFGLAL
                     LGALVGRARLFAWVSRFERVWRSLNLVLVTACIPGYVLAPIPPT"
     gene            complement(621..1517)
                     /locus_tag="PKO06_03880"
     CDS             complement(621..1517)
                     /locus_tag="PKO06_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV68812.1"
                     /translation="MKYFFWLLLWFSLSLSCVQAGDGESTPIQVGFSSTTLSSVDVND
                     AVVALKVWFEQILSAEGLNWKFETTVYQDPRELDAVVREGRVDVLTVSTMEYLRLASG
                     GALIRPLTSEASGMMQDMQYLLVHVQSPIQSLEQLKGKKIIIDARHAGETPMMWLEVE
                     LAKRNLPRAEKFFSSVKRVKEKPSLAILPVFFQQADACLVRQQGLDTVAELNPQVGKN
                     VRILMKSEPFLRGVTGFYKDFAATRIEKIVSSSQKLDKSSKGLQVLTLFKVDNILPMP
                     DGALDSSARLKADYERLVEKRK"
     gene            1754..3331
                     /locus_tag="PKO06_03885"
     CDS             1754..3331
                     /locus_tag="PKO06_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68813.1"
                     /translation="MLLINPPVSRPCEAPAGMARLAGRLREAGHQISLWDANLEGFLQ
                     TIGIEPASDDTWTRRAWRDRERHIRDLRSGEAFAHPDTYARVIGDLERLLSRHPASIQ
                     GWHIGLTDLTHERLQPVRSTDLARAAIEPETNPFYRWFRHVWGNRFAGLNFDLIGISL
                     NYLDQALTASALAGALKTLTPKSRIVMGGGLITSWLSRPGFINPWSGIVERLIAGPGE
                     EALVRLAEEHHHGQQALRSETNSFSLLSTYSGPSENPPGRTYDFFGLPLDQYLSPGIT
                     LPVSLSHGCFWHTCSFCPETAEGNRYQPVHDDIQLLQTLTQRHHQRLLHLLDNALSPR
                     FLRQAAEQAIGAPWYGFARFEEPLDNLDFCRALRRHGCVMLQLGLESGDQSVLDDLHK
                     GISLDRACRILDNLKLAGIGTYVYLLFGTPAEDEASARRTLDLVARHAETIDFLNLAI
                     FNLPIGAGEASTLDTMPFSEGDLSLYVDFKHPKGWNRAHVRRFLDREFRRHPAITPII
                     KRDPPAFTSSHAPFFLP"
     gene            3480..4127
                     /locus_tag="PKO06_03890"
     CDS             3480..4127
                     /locus_tag="PKO06_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68814.1"
                     /translation="MSTQSRFSELKYALTLLFVAPIILGVVLIAACAFDAIPLSIMIR
                     LGMVNITSTSNESATRSIMNQLKNALICYHADIGRWPHVRSQISSETMALADTYCLYD
                     TVSRNVLFNSRVGYPFESNGLTTESYQKRWKGPYLDGTPEECMRDAWEKRIRFELNGT
                     PPSGPDTGRTKKLSILYLHSAGSDGLFDPLSKAVDPAYAGDDIIMQVARTKVPFE"
     gene            4229..4864
                     /locus_tag="PKO06_03895"
     CDS             4229..4864
                     /locus_tag="PKO06_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68815.1"
                     /translation="MNRKNFGSELAHLFTTLVGFFVVLFVSLLVGSAYGFVPISVLER
                     LGVVSVSHGQGKASATRAQMYNLKSALVNYANDLGRYPHTGEQLSSATILLAQRYCLD
                     DHASRNVLVSDSVSRPFEKHGLSDELYKKRWKGPYMDGDPDEFMRDAWDTRIKYTLAW
                     GPAASSSPAVSLVLQSAGPDGVFDPPADCLVASYAGDDLITRVSKLKLPKE"
     gene            4967..>5516
                     /locus_tag="PKO06_03900"
     CDS             4967..>5516
                     /locus_tag="PKO06_03900"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV68816.1"
                     /translation="MRLANSAALLLCLILCAILSAGPICFAANPYQDESIKPASTTGT
                     GNGKSETTVVTENGITNTPTPTRPGNEPPALNPPPEPGKKGITTASPEFKTWLQKVRP
                     QVDAWNFPEGIVNTYGQTITRDDFLKAIIWIESNGVHKSPKGRITTSYVGALGFMQLM
                     PSTAKGLGCDAKDPGQNLLAGCK"
BASE COUNT         1224 a         1697 c         1463 g         1132 t
ORIGIN      
        1 gagttggggg aatgggtgcg agcacataac cgggaatgca ggctgtgact agaaccaggt
       61 tcagcgaccg ccagacacgc tcaaatcgag acacccaggc gaacaagcga gctcgaccga
      121 ccaaggctcc cagcagagcg agtccgaacc agggcccgaa gcaccacacc agggccagca
      181 ccagcgccgt gcgcatctgc cgttgttgac gaagttcccc cgctcgttcc agcagcatct
      241 gcaacccacg gtctgatgga acaaccacag ggtcacttcg ggtgccgtgc agaagacagc
      301 gaacgacggt cgttcctgaa gccgacacct gtagcagata ggagtgtgtc gccagaccga
      361 cttcgcgata cacggggcat tgtgggattt ctccgaagtt ctccggcagt ctgttgcgat
      421 aatcctccaa cctgacttcg acatcgagcg gaggcaaact cgaggccggt cccaactgga
      481 cagcctgctc aatggcgatc tgattcgcca gacaccactt cgtatacatt tcccgccatt
      541 gcggccgata accgaccaaa aagagcgaga ccacggacac gacgctcaag agagcgagtt
      601 ttccgtgtcg cactcgggtc tcacttgcgc ttttcgacca ggcgttcgta atcggctttc
      661 agtcgagcgc tggagtcgag cgcgccatcg ggcataggca gaatgttatc aactttgaac
      721 agggtcaaca cctgcaggcc tttcgacgac ttatcgagtt tctgcgacga agaaacgatc
      781 ttctcgattc tggtggcggc aaagtccttg tagaagccgg tgacaccgcg cagaaatggc
      841 tcggatttca tcaaaatacg cacgtttttt ccgacctgcg gattgagttc ggcgaccgta
      901 tcgagcccct gttgtcgcac gaggcaggca tcggcctgct gaaagaatac gggaagaatg
      961 gccagcgatg gtttttcctt gacccgtttc acggagctga agaacttctc ggcccgcggc
     1021 agattacgct tggccagctc gacttcaagc cacatcatcg gagtttcgcc ggcatggcgg
     1081 gcgtcgatga tgattttctt gcccttgagc tgttcgagcg actgaatcgg cgattgaaca
     1141 tgcaccagaa gatactgcat gtcctgcatc atgccgctcg cttcagaggt caacggcctg
     1201 atcagggcgc ccccggaagc caaccgcagg tattccatcg tcgagacggt cagcacatcg
     1261 accctgccct cacggaccac ggcatcgagt tcacgcgggt cctgatagac cgtggtctcg
     1321 aacttccagt tcaagccttc agcgctgaga atctgctcaa accagacttt gagtgcgacg
     1381 accgcatcgt tgacatcgac actcgacagc gtggttgacg aaaagccaac ctgaatcggc
     1441 gtcgattcgc catcacccgc ctgaacacaa gaaaggctga gcgaaaacca cagcagcaac
     1501 cagaagaagt atttcatggt ttccacctct aattccatta tacctgagta gaccgccttt
     1561 tgcctccgca ccagggcaaa cgggaacttt tttcgcgtgt gacgggcact tggactcccg
     1621 tttgccctgc ctccgcacaa cgtgatgaac agcactctct tgtctcagag ctgatccaga
     1681 accgatggac ccgtgataca ctgccgacgt tttgcacgag caactctgga gcgactaggt
     1741 gccaggaaca aggatactgt tgatcaaccc gccggtgagc cgcccctgcg aagctcctgc
     1801 gggaatggct cgtctggccg gtcgacttcg tgaggccggg catcagatct cgctctggga
     1861 tgccaatctc gaaggatttc tgcagacgat cggcatcgag ccggcgagcg acgacacctg
     1921 gaccagaaga gcctggcgag accgtgaacg gcatatccgt gatctgcgtt caggggaggc
     1981 gtttgcacat ccggacacgt atgcccgtgt gatcggcgat ctcgagcggc tcctctcacg
     2041 ccaccccgca tcgattcagg ggtggcacat aggcctgaca gacctcactc atgaacgtct
     2101 tcagccggtg cgctccacgg atctggcccg ggcagccatc gaaccggaaa ccaatccgtt
     2161 ctaccgatgg ttccgacacg tctggggcaa tcgctttgcc ggcctgaact ttgatctgat
     2221 cggtatctcg ctgaactacc tcgatcaggc actgaccgcc tcggcgctcg cgggagcact
     2281 caaaacgctc accccaaagt ccaggatcgt tatgggtggg ggcctcatca ccagctggct
     2341 ttcacgaccc ggattcatca acccctggtc ggggatcgtc gaacgtctga ttgccggccc
     2401 cggagaggaa gcgctcgttc gcctggccga agagcatcac cacgggcaac aggcgttacg
     2461 ttcagaaaca aactctttct cattactgtc aacatattca ggcccatctg agaatccacc
     2521 aggtaggaca tatgactttt tcggcctccc gctcgatcag tatctttctc ccggaatcac
     2581 tcttccagta agtctttcgc atggatgctt ctggcacacc tgttcgttct gccctgaaac
     2641 cgctgaaggc aatcgctatc agccggttca tgacgacatt cagcttctgc aaaccttgac
     2701 acaacgtcat caccagcgcc tgctgcatct gctcgacaac gctctgagcc cacggttttt
     2761 acgacaggcg gcagagcagg ccatcggcgc tccctggtat ggcttcgctc gcttcgagga
     2821 gccgctcgac aacctggatt tctgcagggc cctgagacgc cacggttgcg tcatgttgca
     2881 gctggggctg gaatcaggcg atcaaagtgt tctcgacgac ctgcacaagg gcatttcact
     2941 cgaccgcgcc tgccggattc tcgacaacct gaagctggcc ggcatcggca cctacgtgta
     3001 tctgctgttc ggcacgccgg ctgaagacga agcctctgcc cgacggaccc tggatctggt
     3061 ggcacggcac gcagagacga tcgactttct caatctggcc atcttcaatc tgcccatcgg
     3121 cgccggcgaa gccagcactc tcgacacgat gccgttttcc gagggcgatc tcagtctcta
     3181 cgtcgatttc aagcacccga aaggctggaa cagagcccac gtaaggcgtt ttctcgatcg
     3241 cgagttccgt cgtcaccccg ccatcacgcc catcatcaaa cgcgacccgc ccgccttcac
     3301 ctccagccac gcgccgtttt tcctgccctg atcggctgtc attttcatgg gcgcacctct
     3361 cgcgcgtcac atctgtcgtc gcagctcccg aacctagcgg ttcgcaacaa atcgggaggt
     3421 tttccccatt gccaaccgcg gcggcatcgc tatattggat gcagaaacgg gaggtcgtca
     3481 tgagcacaca aagccggttc agcgaactga agtatgcact gaccctgctg ttcgtagcgc
     3541 ccatcattct cggggtcgta ttgatcgcgg cctgcgcctt cgatgccatt ccgttgagca
     3601 tcatgatacg actcgggatg gtcaacatca ccagcaccag taatgagtcc gccacgcggt
     3661 cgatcatgaa ccagctgaaa aacgccttga tctgttacca cgccgatatc gggcgctggc
     3721 cacatgtcag atcgcagatc tcgtcagaaa cgatggctct cgcagatacc tattgtctct
     3781 acgatacggt cagccgcaac gtactcttca actctcgggt cggatacccg tttgaatcga
     3841 acggcctgac aacagagagc tatcaaaaac gctggaaagg tccatatctg gatggcactc
     3901 ccgaagaatg catgcgtgat gcctgggaaa agcggattcg tttcgagctc aacggcactc
     3961 cgccttcagg cccggatacc gggagaacaa agaagctttc cattctgtat cttcactccg
     4021 ccggcagcga cgggttgttc gatccgctga gcaaggctgt cgatccggca tatgcgggcg
     4081 acgacattat catgcaggtg gcgcgtacca aggttccttt cgaatgatcg cggctgttga
     4141 ccaacttcac gccctgcgaa ccatcacgat tcgcccttgc caactcgcgt ttctcgacta
     4201 aactggtcgt aggtcgcagg agggcagcat gaaccgaaaa aacttcggca gtgaactggc
     4261 acacttgttc acgactctgg tcggattctt tgtcgttttg tttgtgagtt tgctggtggg
     4321 ttccgcatac ggatttgtcc ctatctccgt tctcgaaagg ctcggggtgg tatcagtcag
     4381 tcacgggcaa ggcaaggctt ccgccaccag agctcagatg tataatctga aatccgctct
     4441 ggtcaactac gccaacgacc tcggccgtta tccgcatacg ggcgagcaac tctcgtcagc
     4501 aacgattctg ctggcacaac gatactgtct cgacgaccac gccagccgga acgtcctcgt
     4561 gagcgactcc gtctccagac cattcgaaaa acacggactc tcagacgagc tttacaagaa
     4621 gcgatggaag ggtccgtaca tggacggaga tcccgacgag ttcatgcgag acgcctggga
     4681 tacgagaatc aaatacaccc tggcctgggg acccgctgcc tcgtcaagcc cggcagtgtc
     4741 tctcgtgctt caatccgcag gccccgacgg ggttttcgat ccaccggccg attgccttgt
     4801 cgcgagttat gctggcgacg acctgatcac gcgagtcagt aaactcaaac ttcccaaaga
     4861 gtgacgccac atcggagcgc attcaagggc tcacactcat ctcgtaaact tttttccctg
     4921 atttctggta gagttggagg gtatgattct catcaggagg acagccatgc gccttgccaa
     4981 ctccgccgct ctgttgctct gtctcatcct ctgcgccatt ctctccgccg gacccatctg
     5041 tttcgcggcc aacccgtatc aggatgagag catcaagccc gcctccacaa ccggaacggg
     5101 caacggcaag agcgaaacca ccgtcgtcac cgaaaacggc attaccaaca cgccgacgcc
     5161 aacgcgcccg ggcaatgagc cgccggctct gaatcccccg cccgaaccag gaaaaaaggg
     5221 aattaccacc gcatcgcccg agttcaagac ctggctgcag aaggtgcgcc cacaagttga
     5281 cgcctggaat tttccggaag gaatcgtcaa cacatacggg cagaccatca cccgcgacga
     5341 cttcctgaaa gccatcatct ggatcgagag caacggcgtg cacaagagtc cgaaaggaag
     5401 gatcaccacc agttacgttg gcgctctggg tttcatgcag ttgatgccct cgaccgcgaa
     5461 aggtctgggg tgcgatgcca aagacccagg ccagaacctg cttgccggat gtaaat
//
LOCUS       DAORHD010000125         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15265_length_5484_cov_3.414441, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000125 DAORHD010000000
VERSION     DAORHD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15265_length_5484_cov_3.414441"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="galT"
                     /locus_tag="PKO06_03905"
     CDS             complement(<1..993)
                     /gene="galT"
                     /locus_tag="PKO06_03905"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV68817.1"
                     /translation="MRERTEERMSELRFNTVTGDWVIIASERAKRPADFKPKTERPPL
                     PEHSPNCPFCPGNEKMSADETLRYNDSRGNWLVRSVVNKFAALAPVEGFACDASGIHR
                     RCTGYGRHEVIIESPQHHLTTALQPQSQINLILRAYRERFRAFFADEHIEHVIAYKNH
                     GPSAGTSLEHPHSQIVGLPVVPAQVKGRLSNSHQHLERQGDCLFCQTLVAEEREGNRL
                     VAQNDSFTAFIPYAALSPFHLWVFPRTHQACFGNISDRTLEDLGRIMKDVLTRLYKGL
                     SNPDFNYVIRSAGLRDLKGDGFHWYISIVPRLNLAAGFELGTGMYVNPSIPETSA"
     gene            complement(1057..2478)
                     /locus_tag="PKO06_03910"
     CDS             complement(1057..2478)
                     /locus_tag="PKO06_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV68818.1"
                     /translation="MSVILVSAAAISWAKAVNREGRKSYLDAFLKDVGGIQNARFFPI
                     EHLGTASIKAYCTAQGIPTETVNAVASYHTCWEETFDAIRRVALRLGAPTILGFSGSN
                     VVLEENLWLANACKQLWPDVSIIFGQDFATINAERLLRDYPIVDYVGRGFGELLVAEL
                     FRIRTSKVSALETISGLAWRDQGGKIRQNPFSPVSLKSLPWVARDDVHIANYHGYGVA
                     VSTARGCPYACTYCASGGVHRVLGRAAYQTREIDDVLEEVAWLYKDHAVRHLVITDEV
                     FFTTSEESKERARAFACGLRDRGLEVQFMIDARVDALDPTVLKPLYDAGLRQMFIGIE
                     TGDEAQRARYGKAYPPGTDVKKNLQGVLDLGIRIIPGVLTFHPETTMSELDQTFSLIS
                     DLGIARVFSFLNRVNPIPGTHLHEEFRHKGYLRREWPIAEFVFRDASIQALHDEFWSL
                     SAQPEVRTEDFLQVFRRHLNKHT"
     gene            complement(2506..3579)
                     /locus_tag="PKO06_03915"
     CDS             complement(2506..3579)
                     /locus_tag="PKO06_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV68819.1"
                     /translation="MSEQTNIETNTGSGTQSTAKVLVPGKTALVTGGGGFLGKAIVKQ
                     LLEKGMKVRSFSRGAYPELEKMGVECFRGEISDPAAVEKACAGCDLVFHVAAKPGIWG
                     PYEEYERINYRGTETVVNACKKLKVPRLIYTSSPSVVFNGQDMQNADETVPYPDHYEA
                     HYPKTKAMAEKLVLAANSAELATTSLRPHLIWGPGDKHLVPRLVKAAREGRLKKVGEG
                     TNKVDAVYVDNAAEAHLQAAEHLEFGKPPAGKAYFITNDDPRLCWDIVNSIIGAAGLP
                     PLTSSINQSVAWVIGWAMETVYGLAGWKEEPRMTRFLANELATHHWFNIAAAKRDFGY
                     QPRISMEEGMKRLADWYKQNPNA"
     gene            complement(3608..5284)
                     /locus_tag="PKO06_03920"
     CDS             complement(3608..5284)
                     /locus_tag="PKO06_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HNV68820.1"
                     /translation="MTNKSPIGVNMALALHEKAQQLPHKRAVIFPYTRDEAGRVAYTQ
                     FTFKQLEQETDRYARGLTRAGIKRGTRTVLMLKPSLEFFALTFALFKIGAVVVLIDPG
                     VGLKNLGICLREAEPEAFIGVPKAHAARVLLRWAPHSIKINITHGLRLFWTGWILSDL
                     RDNSTEPFPMTLFPPDEMGAIMFTSGSTGVSKGVVYTHGILTSQVQYLQQIYGFDQND
                     IDLATFPLFALFDVCLGMTAIIPDMDATRPADADPVKLIEAIENQGATCMFGSPALVN
                     TLSRYGVNHGIRLTTLKKIISCGAPARNDVLQRFHQMLPDDAEIYTPYGATEALPVSN
                     IGSREILGETHLETAKGGGTCIGKPDTHVQVEIIRITDDVISAWKDDLRVKPGEIGEI
                     AVRGPIVTKSYFKREESTALAKIPVPGTSDLYHRMGDVGRLDAQGRLWFCGRKSHRVE
                     TAEGPLFTIPCEAIFNQHPRVFRSALVGVGDKGSQRPVICIELEKEDSGLDLEQLKKE
                     LLELAQKASHTRTISTFLIHPKFPVDVRHNAKIGRETLAQWAADKLRGKP"
BASE COUNT         1113 a         1625 c         1565 g         1181 t
ORIGIN      
        1 cgcgctggtt tccgggatgc tcgggttgac gtacatgccc gtgccgagtt cgaaaccagc
       61 cgccagattg aggcgcggta cgatcgaaat gtaccagtga aagccgtcgc ccttgaggtc
      121 gcgaagcccg gcagagcgaa tgacatagtt gaagtcgggg ttcgacaagc ctttgtacaa
      181 cctcgtcaga acgtctttca tgattctgcc gagatcttcg agagtgcgat ctgagatgtt
      241 tccaaaacag gcctgatgcg ttcgcggaaa cacccacagg tggaacggtg agagggcggc
      301 atacggaatg aacgccgtga atgagtcgtt ctgcgccacc aggcggtttc cctctcgttc
      361 ttccgcgaca agtgtctgac agaacaggca atcgccctga cgctcaaggt gttgatgact
      421 gttgctcagg cgtcctttca cctgagcggg aaccaccggc aacccgacga tctgtgaatg
      481 cgggtgctcg agcgaggtgc ccgcgctcgg cccgtgattc ttataggcga tgacgtgctc
      541 gatgtgttcg tcggcaaaga aggcgcgaaa ccgttcgcga taggcccgca gaatcagatt
      601 gatctgcgac tggggttgta acgcggtggt cagatggtgc tgaggcgact cgatgatgac
      661 ttcatgccgt ccgtaaccgg tacagcgccg atgaatgccc gaggcatcgc aggcaaaccc
      721 ctcgaccggg gcgagagccg cgaacttgtt gacgaccgat cgtaccagcc agttcccacg
      781 actatcattg taacgaagag tctcatcggc agacatcttc tcgtttcccg ggcagaacgg
      841 gcaattcggt gaatgctcag gcagcggcgg gcgctcggtt ttcggtttga aatcagccgg
      901 tcgtttcgcg cgctcagacg caatgatcac ccagtcgccc gtgaccgtat tgaaccgcag
      961 ttctgacatc ctctcctccg ttcgttcacg tattctgtac caacaacact accgcgattc
     1021 gccgccccac acaactcggg gcagacgcgg gcagggtcag gtgtgcttgt tcagatgacg
     1081 gcgaaacacc tgcagaaagt cttccgtccg aacttccggt tgtgcagaaa gactccagaa
     1141 ctcgtcatgc aaggcttgaa tcgaggcatc gcggaaaacg aattccgcga tcggccactc
     1201 ccggcgcaga tagcctttgt gtcgaaactc ttcatgcagg tgagtgccgg gaataggatt
     1261 cactcgattg agaaaactga agacgcgggc gattcccagg tcgctgatca gcgaaaacgt
     1321 ttggtcaagt tcgctcatcg tcgtctcggg gtgaaatgtc agaacaccgg ggatgatcct
     1381 gatcccgagg tcgagcactc cctgcaggtt tttcttcaca tccgtgccgg gtggatacgc
     1441 ttttccgtag cgagcccgct gcgcttcgtc accggtctcg atcccgatga acatctgtcg
     1501 caatcccgca tcatacaacg gcttcagcac cgtcgggtcc agagcgtcaa cccgggcatc
     1561 gatcatgaac tgcacctcaa gcccgcgatc gcgcaacccg caggcaaagg ctcgcgcccg
     1621 ctctttcgac tcttccgacg tggtgaagaa aacttcgtcg gtgatgacca gatgccggac
     1681 ggcatggtcc ttgtacagcc acgcgacttc ttccagaacg tcatcgatct ctcgcgtctg
     1741 gtatgccgca cgacccagca cgcgatgaac tccgcctgac gcacagtacg tgcacgcata
     1801 cggacaacca cgcgcggtcg acactgcaac accgtacccg tgatagttgg cgatatgcac
     1861 gtcatcgcga gccacccagg gtaaactctt gagagaaacc ggagaaaagg gattctgccg
     1921 aatctttccc ccctggtctc gccatgccag accggatatc gtttcgagag ctgagacttt
     1981 cgaggtgcgg atgcgaaaca gctcagccac cagaagttcc ccgaatcccc tcccgacgta
     2041 atcgacgatg gggtagtcgc gaagcagtcg ttccgcattg atggtggcaa agtcctggcc
     2101 gaagataatg gaaacgtctg gccagagctg cttacaggca ttcgccagcc agagattttc
     2161 ttcgagcacg acgttcgaac ccgaaaaacc gagaatcgtc ggggccccga ggcgcagcgc
     2221 gactcgccgg atggcatcga aggtttcttc ccagcatgta tgatatgacg ccaccgcgtt
     2281 cacggtttcg gtcggaatgc cctgagccgt gcagtaggcc ttgatcgagg ccgttcccag
     2341 gtgttcgatg ggaaaaaagc gcgcgttctg aatgccgccg acatccttca gaaacgcatc
     2401 gagataactc ttgcgtcctt cacggttgac ggccttcgcc caggagatcg ccgcggccga
     2461 aacgaggatc acgctcacga aaacacctcg tggaaatatt cgcgatcagg cgttgggatt
     2521 ctgtttgtac cagtcggcga ggcgtttcat gccttcttcc atgctgatgc gcggctggta
     2581 gccaaagtca cgtttggcgg cggcaatatt gaaccagtga tgcgtcgcca gttcgttcgc
     2641 caggaagcgg gtcatgcgcg gttcttcctt ccagcctgcc agcccataca cggtttccat
     2701 ggcccagccg atgacccagg ccacggactg attgatggaa gaagtcagcg gcggcaggcc
     2761 ggcggcaccg atgatcgagt tcacgatgtc ccagcagagc cgcgggtcat cgttggtaat
     2821 gaaataggct ttgcccgcgg gcggcttacc aaattcgaga tgttcggcgg cttgcagatg
     2881 cgcctcagcc gcattgtcga cgtagaccgc atcgaccttg ttcgtcccct cgcccacttt
     2941 cttgagacgt ccttcgcgcg cggctttcac cagacgcggc accaggtgct tgtcgccagg
     3001 gccccagatg agatgcggac gcagagaggt cgtcgcgagt tcggccgagt tcgctgccaa
     3061 taccagcttt tcggccatcg ccttggtttt cgggtaatgc gcttcgtagt gatcgggata
     3121 cggcaccgtt tcatcggcat tctgcatgtc ctgtccgttg aagacgacgc tgggcgagct
     3181 ggtgtagatc aggcgaggca ccttgagttt cttgcaggcg ttgaccactg tctcggtgcc
     3241 gcggtagttg atgcgctcgt attcttcata cgggccccag ataccaggct tcgccgcgac
     3301 gtgaaacacc aggtcgcaac cggcgcaggc tttctcgacc gccgccgggt ctgaaatctc
     3361 gcccctgaaa cattcgacgc ccatcttttc gagctccggg taggctccgc gcgagaagct
     3421 gcgcaccttc attcccttct cgagcagctg cttcacaatg gcctttccga gaaatccacc
     3481 accgccggtc accagagctg ttttgcccgg caccagtact tttgctgttg attgtgtacc
     3541 gcttccagtg ttcgtttcaa tgttcgtctg ctcggacata cgttcgtctc ctctcacgtt
     3601 tgagcgttca cggtttgccg cggagtttgt cggcggccca ttgggccagg gtttcacggc
     3661 cgattttggc gttgtggcgc acatcgacgg ggaacttcgg gtgaatcagg aaagtcgaga
     3721 tcgtcctggt atgcgatgct ttctgggcga gctcgagcag ttcttttttc aattgctcaa
     3781 gatcgaggcc cgaatcttct ttttcgagtt cgatgcagat aaccgggcgc tgactgcctt
     3841 tgtcgcccac cccgaccagc gcactgcgaa agacgcgagg atgctgatta aagatggctt
     3901 cgcagggaat ggtgaagagt ggtccttcgg cggtctcgac ccgatgcgac ttgcggccgc
     3961 agaaccagag tcgaccctgc gcatcgagac gaccgacatc acccatgcga tggtacaagt
     4021 cgctggttcc cggaaccggg atctttgcca gcgccgtcga ctcttcacgc ttgaaatagc
     4081 tcttcgtcac gatcggcccc cgaacggcga tctcaccgat ttcccccggc ttcacccgca
     4141 gatcgtcttt ccaggcgctg atgacatcat cggtgattct gatgatctcg acctgcacat
     4201 gtgtgtcagg cttgccaatg caggtccctc cgccctttgc ggtttcgaga tgcgtctcgc
     4261 caagaatctc acgactgccg atattgctga cgggcagtgc ttcggtcgcc ccgtacggtg
     4321 tatagatttc cgcatcgtca ggcagcatct ggtgaaagcg ctgcagcaca tcgttgcgag
     4381 ctggcgcgcc gcacgaaatg atcttcttca gcgtcgtcag cctgatgccg tgattcaccc
     4441 cataccggct gagcgtattg accagggcgg gagaaccgaa catgcaggta gcgccctggt
     4501 tttcgatagc ctcaatcagt ttgaccgggt cggcatccgc cggccgcgtg gcatccatgt
     4561 ctggaatgat cgcggtcatg ccaaggcaga catcgaaaag cgcgaacaac ggaaacgtcg
     4621 ccagatcgat gtcgttctgg tcaaacccgt agatctgctg cagatactgc acctgactgg
     4681 tcagaatgcc atgggtgtac acgaccccct tcgacactcc ggtgctgcca ctggtaaaca
     4741 tgatcgcgcc catctcgtcg ggcggaaaca gcgtcatggg gaacggttcc gtgctgttgt
     4801 cccgcaaatc gctgagaatc cagccggtcc agaacagccg cagcccatgc gtaatgttga
     4861 tcttgatgga atgcggtgcc cagcgcagca gcacgcgcgc agcgtgggct ttgggaacac
     4921 cgatgaacgc ttcgggctcg gcttcgcgca ggcagatgcc cagattcttc agacccacgc
     4981 ccgggtcgat gagcaccacg accgcgccaa tcttgaacag cgcgaaggtc agcgcgaaaa
     5041 attcaagact cggcttgagc atcagcaccg tacgagtccc gcgtttgatg ccggcgcggg
     5101 tcaaaccacg ggcataccgg tcggtttctt gctcgagctg cttgaacgta aactgggtat
     5161 acgccacccg accagcttcg tcgcgggtat agggaaaaat gaccgcccgc ttgtgcggca
     5221 gctgctgtgc tttttcgtga agcgccagcg ccatgttgac cccgatggga ctcttgttcg
     5281 tcatcgtcga cctctcagaa tctcaggaaa ttctcgcgtc tctacgcggg ttccggctca
     5341 gccggcgatt gtcgcaccgt cgagaggata ccgattgaaa aagtctttca tcagctcgat
     5401 gaccttgtct tcaacgtctt cgagcagata atggctggcc tcagcaaaac tatgggcttc
     5461 cgcgtgcgga aaccgtctga ccca
//
LOCUS       DAORHD010000126         5483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15267_length_5483_cov_3.630066, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000126 DAORHD010000000
VERSION     DAORHD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15267_length_5483_cov_3.630066"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="PKO06_03925"
     CDS             <1..499
                     /locus_tag="PKO06_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68821.1"
                     /translation="GSDWRLRMSDLERYLAPEVDDKAPGTQQGPKMCLKLPKWIEFSG
                     LPAKLNGEHGPETWPVFKKLVELDYEKEEREDRRVFWLESLLPERVGYAEAFVRTVLT
                     KLEKTGLIKQGHDRQKGDFIRIITPIRTPRMILDIPFAQGGIHGAPEQACENRCLRRY
                     LEPES"
     gene            complement(550..1068)
                     /locus_tag="PKO06_03930"
     CDS             complement(550..1068)
                     /locus_tag="PKO06_03930"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNV68822.1"
                     /translation="MKKVLVLIALLCVACLAQAEEPAAVSADLGLAFQQVGDLLKTNL
                     DANAVLGTPVTAGDVTIIPIVVRGFGFGMGEKVRSETEVKSHDKASKEGEKGNKGIGC
                     GAGGMARPIAILVVKKDGQIQLHRLQPEGWISQAVSTLVPVFQNAINKRFEMRKMQME
                     EKKGAPENAPKR"
     gene            complement(1065..2042)
                     /locus_tag="PKO06_03935"
     CDS             complement(1065..2042)
                     /locus_tag="PKO06_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68823.1"
                     /translation="MKIQTPSTVILALLFVIAATIGFAGDSASTSVVPPWVTLPTSTG
                     SGPWEYHSQPLTLGSSTIIVTGAPEYLFVRAPSILSSDTVIVYGRVVPSQLVVSSASQ
                     PMVVPLAHEPRASFLHEMLQASSFMNASGTREVDQTGFSSHEPESVVSLVKRWLHGWS
                     VTPEAWQWKWRVVSGLALASLVWSFLLSAQTSRAAASIQQFPWTSLTYGLIGSGGFVA
                     LLASGWHHPLNLIVPVLAIFPLFPVLFLAPAVTGVSVGRWCLGTCMNGRAWPWVTAVL
                     AVGVWFLILIPVIGPFLFALMLLWGTGGLMLAGVESVRESEIHPPEVVS"
     gene            complement(2137..3906)
                     /locus_tag="PKO06_03940"
     CDS             complement(2137..3906)
                     /locus_tag="PKO06_03940"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68824.1"
                     /translation="MITILEKLGRLACSSNDPQELVRAIVSEVPHIIRVQRVSLVLID
                     RKGFHIPASIGLPQDASVIRHTTEAPIINWVAEHAETLVVKNIDEDERFQHTVRRESY
                     LSKSFVSLPLIEQKKVIGVLSLTDREDGGEFSETDLRSLAIFTNYLAYSLRNSFLLSR
                     LRRNEKLLKRSHNQLKQRFHEIKVLYRLWRGMNNVMDLGSLARFILKRMPAILSVRRA
                     SIIIRDIRTGKLFILEAVGVGKEYEKQPYLSSSPVSEWVIANRQPLLINDINTDPRFR
                     TLRREGYRTGGFICVPLIHKNEVYGVLNVADKRRQGGFAETDMRIMTIFGNQLGVALA
                     NTQLLEEISRRDQIKEEMAIARQIQTQYLPNRRPNLPGIEIGAHLSSAEEIGGDFYDW
                     VERDDGRLAFFLGDVSGKGIPAALTMVLSTTHMAELMMSASDLSVFMKCAHRRICRFS
                     KGVQYVTAGAMILSHDRRRLECALAGHPPMLLWRAATGDLIELSEGGLPLGMYEDLDD
                     YPFGSVEIEPGDSILMYTDGVIDARDRFDKPFTKKRLIEVFTARAREQAFTVVEQLQS
                     ALSSHMTGVPPFDDFTLINLRVR"
     gene            complement(3908..>5483)
                     /locus_tag="PKO06_03945"
     CDS             complement(3908..>5483)
                     /locus_tag="PKO06_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68825.1"
                     /translation="GYRDKTVRLGPEAARLPIAGKAHVFSADQRSTFGGQGLSRANAG
                     KYESISGNGLLHLSFRATEPAMTPFLAGIRTSGGEWSRDLLLDDSGAGSLQIDLSDYE
                     RLVLIPAVATTQADVSRVFAYSFAANAQAMRIYPIPNPAFPTEFIIVVKSESSLAETP
                     KALVRFSNIQAEPALAPADDSRRVFVGNYTIPGTGEGQVTVRVGAETSTFSFYASILK
                     DGGVAHLQLREADVSMRSRVAEDQALLFEGADLEVPDELRVLSRPVELCFNSANMVET
                     RLSFRVQAQTERESQIGIWNQKDSDNHWIRASRTETGWFSAVSREGSYVLVSDRTAPR
                     IHDLRVDLDRDRSMMRARLTDAGAGLKAESVRVEVDGCAIPFQYEPETGLVSADVSHL
                     ARGEHSFDVEVFDRAENRGHAVMKQLLAGPLTILQAVAWPNPCRGAAQLAVILEGSGA
                     DDPDTEVEARVYDVSGARLVTLALEHAGNRTFSSRWDLRTEDGRMVANGLYLFKVTVR
                     RGDSVSKTQGKLAVLN"
BASE COUNT         1251 a         1693 c         1484 g         1055 t
ORIGIN      
        1 tggcagcgac tggcgcctgc gaatgtctga tctcgagcga tatctcgcac cggaagtgga
       61 cgacaaggca ccggggaccc agcaggggcc gaagatgtgt ctgaaactgc cgaagtggat
      121 cgagttttcc ggattgcccg cgaaactcaa cggggagcac ggaccggaga cctggcccgt
      181 gttcaagaaa ctggtcgaac tggactacga aaaggaagaa cgtgaggatc gacgcgtgtt
      241 ctggctcgag tccctccttc ctgaacgtgt cggctacgca gaagccttcg tcaggacggt
      301 tctgaccaag ctcgaaaaga ccgggttgat caaacagggt cacgatcgcc agaaaggcga
      361 tttcatccgg atcatcaccc cgatccgaac cccgcgcatg atcctggaca ttcccttcgc
      421 gcagggaggc attcacggcg ctcccgaaca ggcgtgcgaa aaccgctgtc tgcgacgtta
      481 tctcgagcct gagtcctgag ctctactcac ccccgacccg gacattccgg gtcgggggtg
      541 tcttatcact cagcgtttgg gcgcgttttc gggagctccc tttttctctt ccatctgcat
      601 cttgcgcatt tcgaaacgct tgttgatggc attctggaac accggcacca gcgtggaaac
      661 cgcctgagag atccagcctt ccggctgcag ccgatgtaac tggatctgtc cgtctttctt
      721 cacgacgaga atggcaatcg ggcgcgccat gccaccggca ccacatccga tacccttgtt
      781 acctttctct ccttctttcg aagccttgtc atgcgatttt acttcggtct ccgatcgtac
      841 tttctcgccc atgccaaaac cgaatccacg cacgacaatc ggaatgatgg tgacatcgcc
      901 agccgtcact ggcgtcccca gcacggcatt cgcatcgaga ttggttttga gcagatctcc
      961 cacctgctga aacgccagac ccagatcggc cgaaaccgcc gccggctctt cggcctgagc
     1021 cagacacgcg acacacaaca acgcgatcag taccagtacc ttcttcatga aacaacctcc
     1081 ggaggatgga tttcggactc gcgaacagat tcaactcccg ccagcatcaa cccgcctgtt
     1141 ccccacagca gcatcagcgc gaacagaaac gggccaatga ccggaatcag gatcaggaac
     1201 cagactccca ccgccagcac cgccgtcacc cagggccagg cccgcccgtt catgcaggtt
     1261 ccgagacacc agcgacccac ggaaacaccc gtgaccgcgg gagcaagaaa cagaacagga
     1321 aacagaggaa agatggccaa cacaggaacg atcaggttca gcgggtggtg ccagccactg
     1381 gccagcagag cgacgaaacc gcctgaaccg atcagaccat acgtcagact ggtccaggga
     1441 aactgctgta ttgaagccgc cgcacgtgac gtctgcgctg acagaaggaa actccagacc
     1501 agtgatgcca acgccagacc gctcaccacg cgccacttcc attgccaggc ttcgggggtc
     1561 accgaccagc cgtgaagcca gcgtttcaca agcgatacca ccgactccgg ttcgtgagat
     1621 gagaatccgg tctgatccac ttcacgtgtg cctgaagcgt tcatgaacga actggcctgg
     1681 agcatctcgt gaaggaatga ggcacggggt tcgtgcgcca acggtacgac catgggctga
     1741 gacgcggatg aaaccactaa ctgagatggt acaactcggc catagacgat cactgtatct
     1801 gatgagagga ttgatggtgc tcgaacgaac agatactccg gcgcgccggt gacgatgatc
     1861 gtagaactcc ccagtgtcag cggttgtgaa tgatactccc aggggcccga accagtactg
     1921 gtcggaagcg tgacccatgg cggaacgaca gacgtcgacg cgctatctcc ggcaaacccg
     1981 atggttgccg cgataacgaa caggagcgct agaatcacgg tcgacggggt ctggatcttc
     2041 accgttcttc cctcccacgg ggcaccctcg ccctctcttc aggctagagg acccaacccg
     2101 gtttgtcaaa ccgattcacg ccgcacccgg gtctgatcag cgaacgcgca gattgatcaa
     2161 ggtgaagtcg tcgaagggcg gcacaccggt catatgcgaa ctgagcgcac tctgcagttg
     2221 ttcaacgacg gtaaaggcct gctcacgagc acgcgccgta aatacttcga tcagacgttt
     2281 cttggtgaaa ggtttgtcga aacggtctcg cgcgtcgatg accccatcgg tgtacatgag
     2341 aatcgaatct ccgggctcga tctcgaccga cccgaaggga taatcatcga ggtcttcata
     2401 catcccgagc ggcaaccccc cctcactgag ttcgatgagg tctccggtgg cggcacgcca
     2461 caacagcatg ggcggatgcc ctgccagagc gcattccagc cgtcgtcgat catggctcag
     2521 gatcatggcc cccgcggtca catactgcac tcccttggaa aatcggcaga tacggcgatg
     2581 cgcacacttc atgaaaacgc tgagatcaga tgccgacatc atgagttccg ccatgtgcgt
     2641 cgtcgaaagt accatggtca aggcagccgg aatccctttt ccggaaacat ctccgaggaa
     2701 aaaggccaga cgcccgtcgt cacgctcgac ccagtcatag aaatcgccgc cgatctcttc
     2761 tgcagaactg agatgagcgc cgatctcgat ccctggcagg ttcgggcgtc gattcggcag
     2821 atactgagtt tgaatctgcc gggcaatcgc catctcttcc ttgatctgat ctcggcgact
     2881 gatctcttcg agcagctgcg tgttggccag cgcgacgcca agctgattgc caaagatggt
     2941 catgatgcgc atgtccgtct cggcgaaccc cccctgacga cgtttgtcgg ccacgttgag
     3001 cacgccatag acctcgttct tgtgaatcag cggcacgcag atgaaaccgc cggtacgata
     3061 gccctcgcga cgcagggtgc gaaatcgcgg atcggtgttg atgtcgttga tcagcagggg
     3121 ctgacggttg gcaatcaccc actccgacac gggtgacgaa gacagatacg gctgtttctc
     3181 atactctttg ccgactccga cagcctcgag aatgaacagc tttcccgtgc gaatatctcg
     3241 aatgatgatc gacgcccgcc gcacggagag aatggccggc atgcgcttca gaatgaaacg
     3301 cgccaatgac cccagatcca taacattatt catgccacgc cacagacggt acaagacctt
     3361 gatctcgtga aatcgctgtt tgagctgatt gtgcgacctc ttcaacagtt tttcgttgcg
     3421 ccgcagacgt gacagcaaaa acgaattacg cagcgaatag gcaagatagt tggtgaaaat
     3481 agccagggac cgcagatcgg tttcggaaaa ctcgccaccg tcctcgcgat cggtcaggct
     3541 gagaacgccg ataaccttct tctgctcgat caaaggcagg ctgacaaagg atttggaaag
     3601 atagctctcg cgcctgaccg tatgttgaaa acgctcatct tcgtcgatgt ttttcacgac
     3661 cagagtttcg gcatgttcgg ccacccagtt gatgatcggg gcttccgtcg tatggcgaat
     3721 gacactggca tcttgaggca acccgatcga cgcgggaata tggaaaccct tgcgatcgat
     3781 cagcaccagg ctgacccgct gaaccctgat gatatgcggc acttcactga caatggctcg
     3841 taccagctcc tgcggatcgt tactcgaaca ggccagtcgc cccagctttt ccagaatcgt
     3901 gatcatatca attcagtacc gccagctttc cttgagtttt cgacacgctg tcgccgcgtc
     3961 tcacagttac cttgaacaag tacaatccat tggccaccat acggccatcc tccgtgcgca
     4021 gatcccagcg agacgagaag gtccggttgc cggcgtgttc aagcgccagg gtaaccaatc
     4081 gagctcctga aacatcatac accctcgcct cgacttccgt atctggatca tcagcgccgc
     4141 tcccctcgag aatcacggca agttgcgccg ctccacgaca cggattgggc caggcgacgg
     4201 cctgcagaat cgtgagcgga ccggccagca gctgtttcat gacggcatgg ccgcgattct
     4261 ctgcccgatc aaacacctca acgtcgaacg aatgttctcc acgcgccagg tgcgagacat
     4321 ccgccgacac aagaccggtt tctggctcgt actggaaggg aatagcacat ccatccacct
     4381 caacccgcac cgactctgcc ttcaagccag ccccagcgtc cgtcaaccgt gcgcgcatca
     4441 tggaccgatc gcgatcgaga tcgacccgca ggtcgtgaat gcggggtgcc gtccggtctg
     4501 acaccagcac atacgaaccc tcgcgcgaaa ccgcgctgaa ccagcccgtt tcggtacgac
     4561 tggcgcgaat ccagtgattg tccgagtctt tctggttcca gataccgatc tgactctccc
     4621 tttccgtctg cgcctgcaca cgaaacgaca gccgggtttc gaccatattc gcgctgttga
     4681 agcacaactc caccggtcgc gataacaccc gaagttcatc aggaacctcc aggtctgctc
     4741 cctcgaacag cagggcttga tcttccgcga cgcgcgagcg catcgagacg tcggcctcgc
     4801 gcagttggag atgagcgact ccgccatcct tcagaatcga ggcgtaaaac gagaacgtgc
     4861 tggtctcggc gccaacgcgc acggtcacct gcccctcccc cgtaccggga atcgtgtaat
     4921 tgccgacgaa cacccgacgc gagtcgtcgg ccggtgccaa cgccggttcg gcttgaatgt
     4981 tgctgaagcg caccagagcc ttgggcgttt cagccaatga agactcggac ttgaccacga
     5041 tgatgaactc cgtgggaaag gccgggttgg gaatcggata aatgcgcatg gcctgagcgt
     5101 tggcggcaaa agagtaggcg aacactcgcg acacatcagc ctgggtggtt gccaccgccg
     5161 gaatcaacac caggcgttca taatctgaaa gatcgatctg cagactgccc gccccggaat
     5221 catcgagcag gaggtctcgc gaccattcac cacccgaagt tcgtatacct gcaagaaagg
     5281 gagtcatcgc cggttccgtg gctcggaaac tcagatggag caatccgttg ccggaaatcg
     5341 actcgtactt tccagcgtta gctcgcgaga gtccctgacc cccgaacgtg cttcgctggt
     5401 cagcggaaaa cacatgcgct tttccggcga tgggaaggcg ggcggcttcg gggccaaggc
     5461 gcaccgtttt gtcgcgatac ccc
//
LOCUS       DAORHD010000127         5475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15300_length_5475_cov_3.961993, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000127 DAORHD010000000
VERSION     DAORHD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15300_length_5475_cov_3.961993"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="PKO06_03950"
     CDS             <1..907
                     /locus_tag="PKO06_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68826.1"
                     /translation="AAQTPAPKPAQTAPAPAATASGSGEANPVVLSNQMMITFMERFK
                     SGNFAEARKIVDDMLWGVDKVQDTPTKVYRAFSNLMGKHLFEHQLRTEGTKAEVEWLQ
                     QPYADGYYFQALLAFHEKKVPEALEALQKAISWDPVRAAFHIERGFMMMHLETPPELA
                     MVMATYLKALELADNRQDFAAAMRGIGFVFVEKMDLTAGLAAYMVAQKFDPEDKTAPR
                     EIDYIRAQAPGLTRLLDDGKAISLLSERRIPVTIDPIHVKVLTEIATDLEQKQNTREL
                     KAILRRILDIDPANANAKKKLTALP"
     gene            916..2160
                     /locus_tag="PKO06_03955"
     CDS             916..2160
                     /locus_tag="PKO06_03955"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68827.1"
                     /translation="MNRTTVSWLVVVLWVVVSLTVPRLLPALVPDSASAPELLQAPKA
                     SASALVPQVFVSGAALSPASNPVINVPAPSVASTSMRKPAGGHGAGVGNWWEALAIIL
                     ISDAVMSVENVLIIAILVSAVPPRIRVVATFFGLLAAGVFRVVFASVASILMKFPVVA
                     LLGAIALIFLTVSIFTDTVRQIRKKQAGTGHSVEEKMDFGEVRHELSRIFREKGFWKT
                     PEGQALKTVTTTVILQDILLSLDNVLVVAGNAVGDLSLTVIGVGVSILMMATMANLMV
                     SIVQKYPVVGFVGGLALAKASYNLFHESYDPETAVVAFGSILVFLMFSRVYHKLTTDE
                     EEEVQPLRVELTESAIEPATEAAVAAVSSEKTPVVAAVVGSMTAPELNREFLLELRTC
                     LVRHNETLDRLAAVLQDLKKPR"
     gene            complement(2407..3504)
                     /locus_tag="PKO06_03960"
     CDS             complement(2407..3504)
                     /locus_tag="PKO06_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68828.1"
                     /translation="MKQSSIRSLVLPLFFTTGLLVSGSVCVYGLSDSTLSLVQQLKSK
                     VNTSESPAPTGSKPALELNAPPPIVSPVPSQPEPAIKPQTQPTAEPAPALSEETERPA
                     KKKRDRRKKETPTSVAKPAETERKPAVEEPKQPEPVFGPGPNQARTQRDRAEPAPTIP
                     RQESPRTSEASEMVKHIMEKLATVKQGRQPAGAKDKGKPAAAPKEESAINITSDYSAP
                     VTEAKPTPAPAAAPALTPAPAPAPAAPQPPTVKAPASHGIERSFGELSDDELIQYAKE
                     YVWSSEKSRKHNPPPTPPYRPKKKNTDAKADKSKTQTKTSSDKVAATKAVDKTATVKE
                     SKSAKPATKAGSKTTGSSSSTKKTSSKKKAR"
     gene            complement(3540..4040)
                     /locus_tag="PKO06_03965"
     CDS             complement(3540..4040)
                     /locus_tag="PKO06_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV68829.1"
                     /translation="MKLRTCLSALLLGLTLAAPLSAGPFPLSEVIKRTKGERFMSEPN
                     PYNSHPGVQMMVEFGIASWYDCPTKPEYINNNKYGIRDHIYPVAHKTLPFGTLIKVIN
                     PANGKKIMTRVIDRGPFIAGRIIDLDRPGAQALGIGGIGKIILKIYKVDPKQVAQARQ
                     SSPSTL"
     gene            complement(4245..4703)
                     /gene="ndk"
                     /locus_tag="PKO06_03970"
     CDS             complement(4245..4703)
                     /gene="ndk"
                     /locus_tag="PKO06_03970"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010878270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNV68830.1"
                     /translation="MERTFVIIKPDAVQRGLVGEIITTFERKGLKILGMKMLTITQSQ
                     AERQYAIHKGKPFYDSLVKFMTSGPCVPIVLEGKNAIHLVRKLVGATDPAQSEPGSIR
                     GRYSVDMKHNLIHASDSPESVKHELPIFFTEQELMTYEMSWSPWLYSQVI"
     gene            complement(4730..5197)
                     /locus_tag="PKO06_03975"
     CDS             complement(4730..5197)
                     /locus_tag="PKO06_03975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV68831.1"
                     /translation="MTGPAPKPAEECGLNISPNGLSIHMRIPSEILYLRNALITVKEI
                     CDHYDLEVGLSHRIVLALEEALLNTVEHAYLGNPGVIDVLFAVNEEEFKVVVEDYGCG
                     ISSKDPEFVEEEPPERGRGLTILRGMADESLVDSTIGQGTRATMLFRMAGARA"
     gene            complement(5231..>5475)
                     /locus_tag="PKO06_03980"
     CDS             complement(5231..>5475)
                     /locus_tag="PKO06_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV68832.1"
                     /translation="NTVNEVSIAFARAPQGIDFNSVDGDPVHLIFLLAAPIESGSLYL
                     KLLARISRLLRYQDLIEDLKKATSKEEVIRIIAAKE"
BASE COUNT         1068 a         1458 c         1635 g         1314 t
ORIGIN      
        1 cgccgcccag actccggcgc cgaagccggc tcagacggcc cctgctcctg ctgccacagc
       61 atcgggcagc ggtgaagcca accctgtcgt cttgtcgaat cagatgatga tcacgttcat
      121 ggaacgcttc aagtccggca actttgctga ggctcgaaag atcgttgacg acatgctctg
      181 gggggtcgac aaagtgcagg acaccccaac caaagtctat cgcgccttct cgaatctcat
      241 gggcaagcat ttgtttgaac accagcttcg caccgaaggc acgaaggcag aggtcgaatg
      301 gctgcaacag ccatacgccg atggctacta ctttcaggcc ttgctggcgt ttcacgaaaa
      361 gaaagtgccc gaagcgctcg aagcactcca gaaggccatc tcctgggatc ccgtgcgcgc
      421 cgcgttccat attgaacgtg ggttcatgat gatgcatctc gagactccgc ctgaactggc
      481 gatggtcatg gcgacctatc tgaaagcact cgaactggcc gacaaccgcc aggatttcgc
      541 cgcggcgatg cgcggtatcg gctttgtctt cgtggaaaaa atggatctca ccgccggact
      601 tgctgcctac atggtggcgc agaagttcga ccctgaagac aagaccgccc ctcgcgaaat
      661 cgattacatt cgtgctcagg cgccggggct gactcgattg cttgatgacg gcaaggccat
      721 ctcgttgctc tctgagcgcc gcattcccgt gaccattgat ccgatccatg tgaaggtgct
      781 gaccgagatc gccaccgatc tcgaacagaa acagaatacg cgcgaactga aggccatcct
      841 gcggcggatc ctcgatatcg atccggcgaa tgccaacgcg aaaaagaaac tgaccgcgct
      901 tccctgacac gttccatgaa ccgcaccact gtttcgtggc tggtggtcgt tttgtgggtg
      961 gtcgtctcgc tgacggtgcc acgtctgctc ccggcgctgg tccccgattc cgcgtctgcc
     1021 cctgagttgc tgcaggcgcc aaaggcgtca gcttcggcac tggtgccaca ggtgttcgtt
     1081 tcgggcgctg ctctgtctcc ggcctcaaac ccggtcatca atgttcccgc gccgtcggtg
     1141 gcttcgacgt cgatgcgaaa acctgccggt gggcatggag ccggcgttgg caactggtgg
     1201 gaagcgctcg cgatcatttt gatcagtgat gccgtgatga gcgtcgaaaa tgtgctgatc
     1261 attgccattc tggtttccgc agtgccgcca cgcattcgcg tggtcgccac gttctttggt
     1321 ctgctcgcgg cgggcgtgtt tcgggtcgtg ttcgcctctg tggccagcat tctcatgaag
     1381 tttccagtgg tcgcgcttct cggcgcgatc gccctgattt ttctcaccgt ttcgatcttt
     1441 accgacacgg tgcggcagat tcgcaagaag caggcgggaa caggacattc cgtcgaagag
     1501 aagatggatt tcggcgaagt gcggcacgaa ctgagtcgca ttttccgtga gaagggcttc
     1561 tggaaaacgc ccgaagggca ggctctgaag acggtgacca ccaccgttat ccttcaggac
     1621 attctgctgt cactcgacaa cgtgctggtt gttgccggaa acgcagtcgg tgacctcagt
     1681 ctcacggtga ttggcgtcgg agtatcgatt ctcatgatgg cgacgatggc gaatctcatg
     1741 gtgagtatcg tgcagaagta tccggtagtg gggtttgtgg gtgggttggc tttggcaaag
     1801 gcgtcctaca acttgttcca cgagtcctat gacccggaaa ccgccgtcgt ggcctttggt
     1861 tcgattctgg tgttcttgat gttttcccgg gtgtatcaca agctcacgac tgacgaggaa
     1921 gaagaggtac agccgctgcg cgtggaactt accgaatctg cgatcgagcc cgccaccgaa
     1981 gccgctgtag ccgcggtttc cagcgagaag actccggtgg ttgcggcggt ggtcggttcg
     2041 atgaccgctc cagaactgaa tcgcgagttt ctgcttgaac tacgcacgtg tctcgtgcgc
     2101 cataacgaaa ctcttgatcg tctggccgcg gtgcttcagg acctgaagaa acctcgctga
     2161 ttgctgagcc gtccgggctg tgtgtggttt gcctgaagtg acatgcgaac gcaacacatc
     2221 catggcgcct ggtgttcggt cgtcagcgcc tcggacaata tcgtccgagt tccgccagaa
     2281 ttgagatcat gggttcgctt cctggttggt gatgaggggt agcatggata gcccctcaaa
     2341 agcgacaccc ctctcgtcgc cgtttcagag aggggtgtcg aacgcttgcc ctgtattgaa
     2401 ggtcggtcag cgagctttct tcttgctgct ggtcttcttg gtgctggatg atgagcctgt
     2461 ggttttgctg ccagccttgg tcgcaggctt tgctgacttg gactctttga cggttgctgt
     2521 tttatccact gccttggttg cggccacttt gtccgagctg gttttcgtct gagttttgga
     2581 tttatcagct tttgcgtcgg tattcttttt cttaggtcga taaggtgggg tcgggggcgg
     2641 gttgtgtttg cggctctttt ccgagctcca gacatattct ttggcatatt ggatcagttc
     2701 gtcgtcgctg agctctccaa acgagcgttc gatcccatga ctcgcaggag ctttcaccgt
     2761 tggcggctga ggggcagccg gcgcaggagc aggcgcggga gtgagagccg gtgctgctgc
     2821 cggtgcgggg gtgggcttcg cttccgtcac cggagccgag taatctgagg tgatattgat
     2881 cgctgactct tctttggggg ctgccgcggg ttttcctttg tctttcgcgc ccgcgggctg
     2941 tcgtccctgc ttcaccgttg ccagcttttc catgatgtgc ttgaccatct ccgaagcttc
     3001 cgaagtccgc ggagactcct gacgtggaat ggttggcgcg ggttctgccc ggtcgcgctg
     3061 cgtgcgcgcc tggttggggc cgggcccgaa tacgggttca ggttgcttcg gctcttccac
     3121 cgcgggcttt cgctctgttt ccgctggttt ggcgaccgac gtcggtgttt ccttcttccg
     3181 gcgatctcgt ttctttttcg ccggtctctc tgtttcttca ctgagagccg gagcgggttc
     3241 cgctgtcggt tgggtttggg gtttgatcgc cggctctggt tgtgagggca ccgggctcac
     3301 gatcggaggt ggagcattca gttccaaagc gggttttgat ccggtcgggg cgggcgactc
     3361 ggaggtattg accttgctct tgagctgttg gaccagactc aaggtgctgt cagacaggcc
     3421 gtagacgcag actgaaccgg aaaccagaag cccggtggtg aagaacagcg ggagaacgag
     3481 cgaacgaatc gacgattgtt tcatgaactc gaccttcatg gaaagaatgg tgaagcgact
     3541 tacagggtcg acgggcttga ctgccgtgcc tgggcgacct gcttgggatc gaccttgtag
     3601 attttcaaaa tgatcttacc gataccgccg ataccgaggg cttgagcgcc cggtcggtca
     3661 aggtcgataa tccgaccggc aatgaacgga ccgcggtcaa tgacgcgagt catgatcttc
     3721 ttgccgtttg ccggattgat cactttgatc agggtgccga agggaagcgt cttgtgggcg
     3781 acgggataga tgtggtcgcg gatgccatac ttgttgttgt tgatgtattc aggcttcgtg
     3841 gggcagtcat accaggaagc aatgccaaac tccaccatca tctgaacgcc agggtggctg
     3901 ttataggggt tcggttcact catgaatcgc tcgcccttgg tgcgtttgat cacctcagaa
     3961 agcgggaacg gtccagctga cagaggggcg gccagggtaa gacccaggag cagcgcgctc
     4021 aggcatgttc gtaacttcaa aacgtctacc tcctcgaaca gtttgtctgc agaaacattc
     4081 gttcggccag aaacggtcga gcggtgtctg cggaacgggg ggagatcagc atagcaaagc
     4141 cgcgggaatt ttgcaaaccc gcaccagagg caaagtccga aaaaaaagaa cccccggaac
     4201 accgggggca aagatgagag gatctttgag aggatgcttg aaaatcagat gacttgagaa
     4261 tacagccatg gcgaccagga catctcgtag gtcatgagtt cctgttcggt aaagaaaatc
     4321 ggaagttcat gtttcacgct ttcaggtgaa tcggaggcgt gaatcagatt gtgtttcatg
     4381 tcgaccgaat agcgcccgcg tatgctgcca ggctcagact gggcaggatc agttgcgccg
     4441 accagcttgc gcacgagatg aatggcgttt ttcccttcga ggacgatggg gacgcagggg
     4501 cctgaggtca tgaatttgac gagcgaatcg tagaaaggtt ttcccttatg aatggcatat
     4561 tgacgctctg cctgtgactg cgtaatggtg agcatcttca tgccgagaat cttcaggcct
     4621 ttgcgctcaa acgtggtgat gatttccccg acaagtccgc gctgaaccgc atcgggtttg
     4681 atgatgacga acgttctttc cattcctctc tacctctcta cgagtttttc tacgctcggg
     4741 ctccggccat gcgaaacagc atggtagctc gtgtgccctg accaatggtc gagtcgacca
     4801 gtgactcgtc agccatgcca cgcagaatgg ttaaaccacg tccgcgttcc ggaggttctt
     4861 cctcgacgaa ttccgggtct ttcgatgaga tcccacatcc gtagtcttca acgacgacct
     4921 tgaattcttc ctcgttcacg gcgaacaaca catcgatcac tcccgggttc ccgagatagg
     4981 cgtgctcaac ggtgttcagc agagcttctt caagcgccag aacgattcgg tgcgagagcc
     5041 cgacttcgag atcatagtga tcgcagatct ctttcaccgt gatgagcgca ttccgcaagt
     5101 acagtatctc ggaagggatg cgcatatgaa tcgaaagccc gtttggcgaa atgttcagtc
     5161 cgcattcttc ggcaggtttg ggtgctggtc ctgtcatacg tttctcccgg aggtgcaaat
     5221 gaaggtgcaa ctattccttc gcggcaatga tgcggatgac ttcttccttc gaggtcgcct
     5281 tcttcagatc ttcgatgaga tcctgatagc ggagcagacg cgagatacgg gccaaaagtt
     5341 tgagatagag actgccagac tcgatcggag cggcaagcag gaaaatcagg tgaaccggat
     5401 caccatcgac ggaattgaaa tcgattccct gcggcgctcg tgcaaaagcg atagagactt
     5461 cgttcacggt attcg
//
LOCUS       DAORHD010000128         5430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15468_length_5430_cov_3.864186, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000128 DAORHD010000000
VERSION     DAORHD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15468_length_5430_cov_3.864186"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PKO06_03985"
     CDS             complement(<1..859)
                     /locus_tag="PKO06_03985"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68833.1"
                     /translation="MNRLAIFERSIAQPNLEKATDLITGGQTVRSIGGCSPQAAHQLL
                     AWTDIPIKTLQEGTDPEGGEFLVVERPPHGRRDIVRSLVDILADLNSPDLTLALEFMN
                     QENSLLEEEAALLIPFLQKQLFSSDQQVRYLARKARDHLLQKYPDLEKRRDGDTASYE
                     PLEGAGTREILLQKMRLGSRFVCFDAIERLTDSGDTTLIEPLMQFLEQESDPFKIAHL
                     VKRIPRLKDQRIAKVLRAYLSHPDPRIVANAVEGFWYIKAPDLVDEFAHLGDSPDNRI
                     RANAIKALFP"
     gene            complement(930..1859)
                     /locus_tag="PKO06_03990"
     CDS             complement(930..1859)
                     /locus_tag="PKO06_03990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV68834.1"
                     /translation="MQERTQQSSIAGLYTLIGGMCLCGMFISIGVPVLFTTPYFFSLR
                     AFAGIVACFSITGIICERLEKTSAALISGMIAATFFVYGCFEVVYALLPSPAGWVEPQ
                     SGWYPLWRELYSYAGNRALQVIPIAALAGLLAFHFPRLWQHRCRIGQPDSPTTILGSS
                     QPQSWKQVAGRLAFYLTIVTVGFLVARGGGSEGSGRLMLFCGRLIGGTTNSLIEELLF
                     RGILQPVFESVISLGAANALQAVFFAVIHFGYVETLTVAALAPEVGRILLYTGIGWFL
                     GRAARETNGLLIPWFFHALVTAAIWVLLTVQGS"
     gene            1924..3177
                     /locus_tag="PKO06_03995"
     CDS             1924..3177
                     /locus_tag="PKO06_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV68835.1"
                     /translation="MMSEAPRERPPRQCLILSRDTNYTVFLELVIGQYGFRAKVTDMV
                     QKLGTLSSSMPTVAWVIDLDGISETIEDLVKLARLKAPDARLVFLSSRFTVEMAQACI
                     RLGAIALLLKPFPVPRLVQTLSSIHQERELLEEDDLISSNDQDRDTPVPSPAKSDTAP
                     KGFSQEPEIGDDPLLRVVNLQCPVCLHHFPTARFKLWVMPVCDTDTDFCPVTTGAVHP
                     ELYSVMVCPNCCFAYYAGKFSGLKIAEPIRREFLSATCTGERKALTAGLVYTGKRTLL
                     HGIKAFDLAALAVQQLRIRNCIKLAGEFYLKASWLSRKVGHQLNERQAQEKALACFEA
                     CYRPYLRQGGRFPGRDVLISRFDKLRNRWKTGPSSSRVSWPASWHGASASARKPSSTL
                     KRWSISRICISTRRCTSTSMPCIGV"
     gene            complement(3223..3351)
                     /locus_tag="PKO06_04000"
     CDS             complement(3223..3351)
                     /locus_tag="PKO06_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68836.1"
                     /translation="MDQQQNPQSPKEVDVVKEATSKVIFAVIVFGAMLFAKFVLGW"
     gene            3631..>5430
                     /locus_tag="PKO06_04005"
     CDS             3631..>5430
                     /locus_tag="PKO06_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68837.1"
                     /translation="MSGRRPREENADDLFDGDFAEVDPSWRQAAILRLAREPEPGYRG
                     VLETALWDQNPRTAFIAYLGLRRLHPPVSGMHEAWLDLFGESVRLLETRAASGPLPLR
                     IAAMQALAFAPGGVFTGVIERWKRDPLSELTTWGSLTEQPPALPVINDSGSGGWPEIQ
                     GVLLAATADRGERVRLMTDLLETARGDDALPVFLALQLEPEPEMTDMLLPWLHAPDTR
                     IAVEAARAVLACGGSGVYVLLLAQVKQAGDPDKRSGFLSVLARTGREEAWQVCCAHLN
                     HEDAAVRLAAVEALARFPRPATDRQQALRERQNDPHGVVAATAHGWLWRLGALESVSR
                     LTQMLSDASAGMRQAAARALALVAPATAVPLLVDLLEVERQGDVLRAALISLRRLLPG
                     RSLPPRVLERLLLQFRRLLSGPDFFRRSQVAVLCGFLGTAAEELLLSSLEEQEHPHVL
                     ASLLAAVARVGSTRVLVFSRFRDHPDPRVRANLMEALLGGGPGGIPFLSQGLRDPSPR
                     VRSGAARSLFALGQLEVVGVLNRMLLTPSPLPVLAACHALGSLMRWHPPALSPDHPLR
                     LQLGTRVRRRQPAAPGTPAWLSEPFLPELLQELA"
BASE COUNT         1032 a         1571 c         1724 g         1103 t
ORIGIN      
        1 acgggaacaa cgccttgatg gcattcgcgc gaatgcgatt gtcaggggaa tcacccagat
       61 gcgcgaattc atcgacgagg tcgggggctt tgatgtacca gaaaccttcg acggcgttgg
      121 cgacgatacg cgggtcaggg tgggagagat aggcgcgaag gaccttggca atgcgctggt
      181 ctttgaggcg cggaatacgt ttcaccagat gcgcgatctt gaacgggtcc gattcctgtt
      241 cgagaaactg catcagcggc tcaatcaggg ttgtgtcgcc gctgtcggtc aggcgctcga
      301 tcgcgtcgaa acagacgaaa cgactgccga gacgcatctt ctgcagcaga atctcccgcg
      361 tgccagctcc ttccagaggt tcataactgg cggtatcacc atcgcgtcgc ttctcgagat
      421 cgggatactt ctgcagcagg tgatcgcgag ctttgcgcgc cagatagcgc acctgctggt
      481 cgctggaaaa cagttgcttc tgcagaaagg gaatcaggag tgccgcttct tcctcgagca
      541 gggagttttc ctggttcatg aactcgagcg ccagcgtgag atcaggagag ttgaggtcgg
      601 ccagaatgtc gacgagactg cgcacgatgt cgcgccgccc atggggaggc cgctcgacga
      661 cgagaaactc gccgccttcc ggatcggtgc cttcctgcag cgtcttgatt ggtatatctg
      721 tccaggcgag cagctggtgc gccgcttgag gggagcagcc gccgatggag cgaaccgtct
      781 gtcctccggt gatcaggtcg gtggcctttt ccaggttggg ctgggcaatc gaacgttcga
      841 aaatggcgag acgattcatg aacctcatcc ttgagtcatg cgtgttgaca ggtcgaagtg
      901 cggccgggtc gaggttcgca aaggatactt caagacccct gaacggtcaa cagcacccag
      961 atggcggcgg tcaccaacgc atgaaagaac cagggaatca gcaggccatt cgtttctcgg
     1021 gcggctcggc cgagaaacca gccgatgccg gtatagagca gaatacgccc gacttcgggc
     1081 gcgagagccg cgaccgtcag ggtttccacg tatccgaagt gaatgacggc gaagaacacc
     1141 gcctgcagag cattggcggc ccccaggctg atcacgctct caaaaacggg ctgcagaatg
     1201 ccgcgaaaca acagttcttc gatcagcgaa ttcgtggtgc caccgatcag acgaccgcag
     1261 aatagcatca gacggccgct tccttcactg ccacccccgc gcgcgaccag aaacccgacc
     1321 gtgacgatgg tgagatagaa ggcgagacgc cccgcgacct gcttccagga ttgcggttga
     1381 ctgctgccca gaatcgtcgt ggggctgtcg ggttgtccga tgcggcagcg atgttgccag
     1441 agacggggaa agtgaaacgc gagaagtccg gcgagagccg cgatgggaat cacctgcagt
     1501 gcgcggttgc cggcgtaact gtacaattcg cgccagagcg gataccaccc ggattgcggc
     1561 tcgacccaac cggcagggct gggcagcagg gcgtagacaa cctcaaagca gccgtagacg
     1621 aagaacgtcg cggcgatcat accgctgatc agggcggctg acgttttctc gagacgctca
     1681 cagatgatgc ccgtgatact gaaacaggcg acgataccgg cgaaggcgcg caacgaaaag
     1741 aaatacggag tggtgaacag caccggaaca ccgatcgaga tgaacatccc acagagacac
     1801 atgccgccga taagcgtgta tagacccgca atggaagact gctgcgttct ttcctgcatg
     1861 ataaaccggc tccggaattc gagagagatt tacttttccg ctcggatgat acatcatggg
     1921 ggcatgatga gcgaagcacc tcgtgaacgc ccaccccggc agtgtctgat tctgtcgcgc
     1981 gacaccaact acaccgtctt cctggagctc gtcatcggcc agtatggctt tcgtgcgaaa
     2041 gtcaccgata tggtgcagaa gctgggaacc ctgtcatcgt cgatgccgac cgtcgcgtgg
     2101 gtcatcgatc tcgacgggat ctcggaaacg attgaagatc tggtcaagct ggccaggctg
     2161 aaagcgcctg atgcccgcct ggtgtttctc agctcgcgat ttacggtcga gatggcccag
     2221 gcttgtattc gactgggagc aattgccctt ctcttgaagc cttttcctgt tcctcgactt
     2281 gtacaaacat tgtcgtccat tcatcaggaa cgagaactgc tggaagagga cgacctcatc
     2341 tccagcaatg accaggatcg tgacactccg gtgccgagtc ccgcgaaatc tgacaccgcc
     2401 ccaaaggggt tctcacaaga gccggagatt ggtgatgacc cgttacttcg tgtggtcaat
     2461 cttcagtgcc cggtctgcct gcaccacttt ccgacggccc gtttcaagct ctgggtcatg
     2521 ccggtgtgcg acacggatac ggacttttgt ccggtgacga ccggtgcggt ccacccggaa
     2581 ctatactcgg tcatggtgtg tcccaattgt tgctttgcct actatgcggg caaattcagc
     2641 ggcctcaaga tcgccgagcc gattcggcgt gagttcctgt cggccacctg taccggtgaa
     2701 cgcaaggcgc tgaccgcggg gctggtctat acgggaaaac ggacgttgct gcatggcatc
     2761 aaggcgttcg atctggcggc tcttgccgtg cagcagttgc gaatcaggaa ctgcatcaag
     2821 ctggcggggg aattctacct caaggcatcc tggctgagcc ggaaggtcgg ccaccaactc
     2881 aacgagcggc aggcgcagga aaaggccctg gcctgtttcg aggcgtgtta tcgcccgtat
     2941 ctcagacagg gaggtcgttt cccggggcgc gacgtgttga tttcccgttt cgacaaactg
     3001 cggaaccgct ggaagaccgg gccatcgtca tcacgggttt cctggccggc gagctggcac
     3061 ggcgcctcgg cgagcgcgag aaagccctcc tctaccttga agaggtggtc aatatcccgt
     3121 atctgcatca gtacacgtcg ctgtaccagc acgtccatgc cgtgtatcgg ggtctgaaag
     3181 aggcaacccc caaggcgatc tgatcagagc gggaaggttc gtttaccagc cgagaacgaa
     3241 cttcgcgaag agcatggctc cgaagacgat gacggcgaaa atcactttgc tggtcgcttc
     3301 cttgacgacg tcgacttcct tgggactttg cgggttctgc tgttgatcca tcggtggcct
     3361 ccattggcgc aaaattgccg aatggacgca gatgtgtgaa gtgtggcccg agaacggaag
     3421 gcggcactca aaatgcgtca ttcggacatt ccgaatggcg gcccgaccat ccgcagtaac
     3481 ctccggagtc gtgatctccg gacccacttt cagtatagca gatctccctg gaattcgcca
     3541 tccggcggtg tcgggatcaa ctggcggttg tcctggaagg gagcgctcgt tataatgcgc
     3601 tcaaggtctg agcaaggagt gtttcgcatc atgagcggcc gtcgcccgcg agaggaaaac
     3661 gccgacgacc tgtttgatgg cgattttgcc gaggtcgatc cgagttggcg acaggcggca
     3721 attcttcgtc tggcgcgtga accggagcca gggtatcggg gcgtgcttga aaccgccctc
     3781 tgggatcaga atcccagaac cgcatttatc gcgtatcttg gcttgcgtcg attgcatcct
     3841 cccgtgtctg gaatgcatga agcctggctt gacctgtttg gcgagtcggt acgtttgctc
     3901 gagactcgtg cggcttccgg gccccttccc ctgcggatcg ccgccatgca ggcattggct
     3961 tttgcccccg ggggcgtatt caccggggtg atcgagcgct ggaagcgtga tccattgtcc
     4021 gagttgacaa cctggggaag tctcacggaa cagcctcctg cgcttccggt gattaacgat
     4081 tcggggagtg ggggctggcc cgagattcag ggggtattgc tggcggcgac ggcagatcgt
     4141 ggggagcgag tgcgcctgat gacagatctg ctcgagacgg cacggggcga tgatgccttg
     4201 ccggtttttc tggcgttaca gttggagccc gagccggaga tgaccgacat gctgctgccc
     4261 tggctgcatg cgccggacac acgcattgcg gtggaagccg cgcgcgccgt gctggcgtgt
     4321 ggtggttccg gggtctatgt actgctgttg gcgcaggtta aacaagccgg tgacccggac
     4381 aaacgcagcg gctttctttc cgttctggcg cgaaccgggc gagaagaagc ctggcaggtc
     4441 tgctgtgccc acctgaatca cgaggatgcc gcggtgcgac ttgccgccgt cgaggcactg
     4501 gctcgctttc cgcgcccggc cacggatcgc cagcaggctt tgcgcgagcg gcagaacgat
     4561 ccgcatggcg tcgtcgcggc aacggctcac ggctggctct ggcgtctggg agctctcgag
     4621 tcggtgtcac gcttgacgca gatgctgagc gatgcgagcg cgggcatgcg acaggcggct
     4681 gcccgggccc tcgctctcgt ggctcccgcg actgcggttc ccctgctggt cgatctgctg
     4741 gaagtcgagc gacagggaga cgtgttacgg gcggctctga tcagtctgcg gcgattactg
     4801 ccggggcgat cgctgcctcc ccgggttctc gagcggttac tgctgcagtt tcggcggctg
     4861 ttgagcggac cagacttctt tcgtcgcagt caggtcgctg tcctgtgcgg gttcctcggg
     4921 actgctgccg aggaactgct gttgtcctca ctcgaggagc aggagcatcc gcatgttctg
     4981 gcttcactgc tggcagcagt cgcccgggtc gggtcgacgc gggttctggt gttcagccgc
     5041 ttccgcgatc atccggatcc acgggtgcgg gccaatctca tggaggcctt gctgggaggc
     5101 ggtccgggcg gcatcccgtt cctctctcag ggcttgcgag atccatcgcc tcgcgtgcga
     5161 tcaggagcgg ctcggtcgct gtttgccctc ggacaattgg aagtcgtcgg ggtgctcaat
     5221 cgcatgctgc tgaccccctc gccgcttccg gtgctggccg cgtgtcatgc gctcggttct
     5281 ctgatgcgct ggcacccgcc cgcgctttcc cccgatcatc ccttgcgtct gcaattgggg
     5341 acccgggttc gccgccgcca gcccgcggca ccgggaaccc ctgcctggtt gtccgagccg
     5401 ttcctcccgg aactgctgca ggaactggcg
//
LOCUS       DAORHD010000129         5421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15520_length_5421_cov_3.509132, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000129 DAORHD010000000
VERSION     DAORHD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15520_length_5421_cov_3.509132"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKO06_04010"
     CDS             <1..653
                     /locus_tag="PKO06_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV68838.1"
                     /translation="VGYTNAGKSTLLNALTGSQVTVHDGLFTTLDPTARRLSLPSGRS
                     AILSDTVGFIQKLPHGLVKAFRATLEDVLNSQVVVHVTDLSHPDALERIGAVNEVLRE
                     IGVLERETIMVFNKTDREAAIAREGLTHDFPGCCFISAKSGDGLDTLLRRLDEIMARE
                     VKTVELIIPAEQPMLREVRQWSRIITEEWLPEGVRLQAEVPTKLVVRLKPWLVTDE"
     gene            785..1840
                     /locus_tag="PKO06_04015"
     CDS             785..1840
                     /locus_tag="PKO06_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNV68839.1"
                     /translation="MPDYLSQTQYQQLRDFFSREFGLFFEPNKLTFLENRVLPLMHEL
                     KCLDLNHFILEVQGDEEKRARLLNELTTNETWFFRHPRHFDILRDDILPPLIREGMQH
                     RKREISVWSAGGSIGAEAFSIAITLQEALRELSEWRVTIVCSDISRAAIQRAQEGIYS
                     LSELRLLSNTLLTRHFIPLDAERFQVKPELRSLIQFEHLNLVSSWPQRDFDIIFCRNT
                     MIYFREATKKALTERFYRVLKPGGYFFISPTETIHWSGENEVERLFLRGEYVYRKRVQ
                     ARDYLLYEFHSPSDLLRALNLLVKASIEYHLRSVPPRGPGSPKRALFFPRSLGVEVES
                     LLTKHGVQTAAKSEFSE"
     gene            1905..2363
                     /locus_tag="PKO06_04020"
     CDS             1905..2363
                     /locus_tag="PKO06_04020"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV68840.1"
                     /translation="MVTTQPVNIRIRVTILVQRPDGRVCLVRHLKDGRRYWLLPGGGQ
                     EPFECMTETARRELWEETRIACGQFEFLGLRESFASEAGRHIVYPVFRGLNPDFSAVE
                     RGEDARVEAVDFLTWEEIRERTVFPEMKTDLERLVRGEPILPFLTLDWIP"
     gene            2377..4947
                     /locus_tag="PKO06_04025"
     CDS             2377..4947
                     /locus_tag="PKO06_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68841.1"
                     /translation="MLEKKYETLIQDCRKRIAANPRDFFAYYQLARALEMSGDTDAAI
                     TVYRDALAINPKDAYAHNFLGLSYRNKGMINEAITEFKKAIMYNASYAFPYANLGVIY
                     KMKGMYDEAIIRFKKAISLKTDFAFSHLQLGLIYRLKKLYDEAIVRLRNAIDINHDYA
                     YAHFALGAVFLEINKPKKAIVEFKKSVLLNPQDYFAFCQLGRAYIALRDHDEAEKVLK
                     TSIRLNPDNPFAYLNLGSAYFAMARFDEAIEATKRVLDINPDLVEARVLLGQSLMRKS
                     MDDAALTELRRALKADPKFPRLHFLMGTVLRKKGDLVKAIEEFKAENAVNPNDAELHL
                     QLGQTYKDKGMLDEATSELKRALYLDATLFRPHEILGEIYDVKGMSDLAVTAWKKASE
                     MRKRYSTTKPREKPAPMVQNKSELEMMIERFKSDVMTAPNNPDAHYKLASAYKNNHMF
                     DEAIIEFRKVLALNPYDSFAHYNLEVIYRLKGVDDPNLTRWKRSVAVNPENAGAHYRL
                     GLAYKEKGMLDEAIREWEKAVAIDPDFSHAHLQLGIAYKCIGQADSAIRSWEKTVELD
                     PEFREAFFHLGKAYLNRGMLEEAVHAIKKTIRLHPQDVESRFLLGLIYKNNGLMEDAR
                     DVLEDLLALKEDHVQALVNLAAVYKNLNQLENAYNCLLSALRLDQTNFFAHFLLGSVY
                     RALGQLERAEEAFRKSLELNPTDAFSQYSLGVIYKNSGRLPLAIKAFEKAIELDSDDS
                     YSYSHLATSLYEQGQLHEAVKAFQHAVEINPEDSYALYNLGMAYMDLHDVDAAIDAFV
                     KVVNLEPNDAYACFNLGLAYEEKRLLPEAKACFQKALQHAQKGTQVERFARESLRRMK
                     "
     gene            4950..>5421
                     /locus_tag="PKO06_04030"
     CDS             4950..>5421
                     /locus_tag="PKO06_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV68842.1"
                     /translation="MSFPSHSRHQADGKNGTVRVSNRNQGAQATAGGLTLNAVRHASQ
                     GQGKPGGRAMMLGMMSGWLSVVLLGALLIMGCDTDNNVKSREGALSGKVVDKAGTALQ
                     YALVSWAEDRTRWGRTDANGKFVVEGVSFGTRQFVVELTGYRTRTFDVNIYSEAI"
BASE COUNT         1217 a         1459 c         1524 g         1221 t
ORIGIN      
        1 tggtcgggta cacaaatgcc ggcaagtcga ctttgttgaa tgccctgacc ggttctcagg
       61 tgaccgtgca tgacgggttg ttcaccacgc ttgaccccac cgctcgacgt ctgtcgttgc
      121 ccagcggtcg gtctgccatt ttgtctgaca ccgtcgggtt tattcaaaag ctcccgcatg
      181 gcttggtgaa agcgtttcgt gccacgctcg aagatgtctt gaattctcaa gtcgtggtcc
      241 acgtgaccga cctttctcac ccggacgctc tcgaacgcat cggggccgtg aacgaagtcc
      301 tgcgtgagat cggggtgcta gagcgagaaa cgatcatggt ctttaacaag accgaccgcg
      361 aagcggcgat tgcacgcgaa gggttgacgc atgattttcc gggctgctgt ttcatttcgg
      421 ccaaaagtgg cgacgggctt gacaccctgc tgcgacgcct cgatgaaatc atggcgcgag
      481 aggtgaagac ggtcgagctg ataatcccgg ctgagcagcc gatgttgcgc gaagtaagac
      541 aatggtcgcg aattatcacc gaagaatggt tgccggaagg agtacgattg caagccgagg
      601 taccgacgaa actcgtggtc cgactcaaac cctggcttgt gaccgatgaa tgaacctgtg
      661 ggtcgcacac gatctcgcgg ggttgcgggg gtactgctcc cgaccatggt ctttttcgtg
      721 acttgcgtgg atgagactat tcgccgaaga cggaatgggc tataatggta ggcatgtgac
      781 gcgcatgccc gactatcttt cacagacaca gtatcaacag ctgcgcgact tcttttcgcg
      841 tgagttcggc ctgtttttcg agccgaacaa actgactttt ctcgagaatc gcgtactgcc
      901 gctgatgcac gagctgaagt gtctcgatct gaaccacttc attctcgaag tgcagggcga
      961 cgaggaaaag cgggctcgtc tgctgaacga gctgacgacc aacgaaacct ggttttttcg
     1021 acacccgcgc catttcgata ttctgcgcga tgacattctt ccccccctta ttcgtgaagg
     1081 gatgcagcac cgcaaacgtg agatctccgt ctggtccgct ggtggttcta ttggtgccga
     1141 ggcgttttcc attgcgataa ctctgcagga agccctgcgt gagctgagtg agtggcgggt
     1201 gacgatcgtc tgcagcgata tttcgcgagc tgcgattcag cgggcgcaag agggcatcta
     1261 ttcgttgtcc gagttacgcc tgctgtcaaa cacgcttctg accagacatt tcatccctct
     1321 tgatgccgaa cgcttccagg taaaacctga actgagaagc ctgattcagt ttgaacacct
     1381 caatctggtc tcgtcctggc cgcaacgtga ttttgacatc atcttctgtc gcaataccat
     1441 gatctacttt cgcgaggcga ccaaaaaggc cctgaccgaa cgtttctacc gggtgttgaa
     1501 accaggcggc tatttcttca tcagtccaac cgagacgatt cactggtcgg gggaaaacga
     1561 ggtcgagcgc ctctttctcc gcggcgaata cgtctatcgc aagcgcgtgc aggcgcgcga
     1621 ctatctgctt tatgagttcc attccccgag tgatctgctg cgcgcgttaa atctgctcgt
     1681 caaagccagc atcgaatacc atctgcgtag cgtgccacca cgtggacccg gttccccgaa
     1741 acgggcgctg ttttttcccc gcagtctggg cgtcgaggtc gagagcctgt tgacgaagca
     1801 cggggtgcag actgccgcga agtcggagtt ttccgagtaa ctgaccgttt tccattcctg
     1861 gcttggagat tttcgcatct ctgccgatac aagggatttg gattatggtc acaactcaac
     1921 cggtcaacat tcgcattcgg gtcaccattc tggtgcagag gcccgatgga cgtgtctgcc
     1981 tggtgcgtca tctcaaggac gggcgccgct attggctgct gcccgggggc ggccaggaac
     2041 cgttcgagtg catgaccgag acggctcgtc gtgagctgtg ggaagaaacg cgcattgcct
     2101 gcggacagtt cgagtttctg ggtctgcgcg aaagtttcgc ttccgaagct ggccgacata
     2161 tcgtctaccc tgtgtttcgc ggcctcaatc ccgatttctc cgcggtcgag cgcggagagg
     2221 atgcccgggt tgaggcggtt gattttctga cctgggagga aatccgggaa cggaccgtgt
     2281 ttccggaaat gaagaccgat ctcgaacggc tggttcgggg ggagccgatt cttccctttc
     2341 tgaccttgga ctggattccg taggctggag gcacgcatgc tggaaaaaaa atacgagaca
     2401 ctgattcaag actgccgcaa acgtattgcc gcgaatccac gcgacttctt tgcctactat
     2461 cagttggcgc gcgcgctcga aatgagcggt gacacggatg cggccattac ggtgtatcgc
     2521 gatgctctgg ccatcaatcc gaaagacgcc tacgcacaca actttctcgg tttgtcttat
     2581 cgcaacaagg gcatgatcaa cgaggcgatc accgagttca agaaagccat catgtacaac
     2641 gccagctacg cgttcccgta tgccaatctc ggcgtcatct acaagatgaa gggaatgtac
     2701 gacgaagcca tcatccggtt caagaaagcg atctcactca agaccgattt cgccttttct
     2761 cacctgcaat tggggctcat ctatcgcctc aagaagttgt acgatgaagc gatcgtacgg
     2821 ttgcgcaacg cgatcgacat caatcatgat tacgcctatg cgcattttgc cttgggtgcc
     2881 gtgtttctcg aaatcaacaa gccgaagaag gcgattgtcg aattcaagaa atccgttctc
     2941 ttgaaccctc aggactactt cgctttctgt cagctgggtc gcgcctatat cgcgttgcgt
     3001 gaccatgatg aggccgagaa agtgctgaag acctcgattc gtctgaatcc ggacaatccg
     3061 tttgcctatc tgaatctggg gtcggcttac ttcgccatgg ctcgtttcga tgaagccatt
     3121 gaggcgacaa agcgggtgct cgacatcaat ccagatctcg ttgaagctcg cgtgctgctc
     3181 ggacaatcgc tgatgcgtaa atcgatggat gatgccgcgc tgacggaatt gcgccgcgcc
     3241 ctgaaggccg atccgaagtt tccgcggctg cactttctca tgggcacggt gctgcgtaaa
     3301 aaaggcgatc tggtgaaagc gatcgaggaa ttcaaggcgg aaaatgccgt caatccgaat
     3361 gacgctgaat tgcatctgca actgggacaa acctacaagg acaagggcat gctcgatgag
     3421 gccaccagcg aactgaaacg ggcgctgtac ctcgatgcga ccctgttccg accgcatgaa
     3481 atccttggcg agatctacga cgtcaaaggc atgagcgatc tggccgtgac cgcctggaaa
     3541 aaagcgtcag agatgcgtaa gcgctactcg acgaccaaac cgcgcgagaa gccggcgccg
     3601 atggtgcaga ataagtccga gttggaaatg atgatcgaac gcttcaaaag cgatgtcatg
     3661 accgctccga ataaccccga cgcgcactac aaactggcaa gtgcctacaa gaacaatcac
     3721 atgttcgatg aagcgatcat cgagttccgt aaagtgctgg ccttgaaccc gtatgattca
     3781 ttcgctcatt acaacctcga agtcatttat cgtctgaagg gcgtcgacga ccccaacctc
     3841 acccgctgga agcgttcggt ggccgtgaat cccgaaaatg cgggcgcgca ttatcgcctc
     3901 ggtctcgcct acaaagagaa aggcatgctc gacgaagcta ttcgcgagtg ggagaaggct
     3961 gtggcgatcg atcccgattt ctcgcatgcg catctgcaac tgggtattgc ctacaaatgc
     4021 atcggccagg cagacagcgc cattcgttcg tgggaaaaaa ccgttgagct tgatccggaa
     4081 ttccgtgagg ccttctttca tctcgggaaa gcctatctca atcgcggcat gcttgaagaa
     4141 gcggtacatg cgatcaagaa gacgatccgc ctgcacccgc aggatgtcga aagtcgtttc
     4201 ctgttgggct tgatttataa aaacaatggg ttgatggaag atgcccgcga tgtgctcgaa
     4261 gatctgctgg cactcaagga agaccatgtg caggcactgg tcaacctggc cgccgtgtac
     4321 aagaatctga atcagctcga aaatgcgtac aactgtctgc tgtcggcctt gagacttgat
     4381 cagaccaact tctttgctca cttcctgctg ggcagcgtct atcgagcact ggggcagctc
     4441 gaaagagccg aagaggcatt tcgcaagtcg ctcgaactca acccgaccga tgccttttct
     4501 caatacagcc tcggggtcat ttacaagaac tcgggcagac tgccgctggc gatcaaggcg
     4561 ttcgaaaagg ccatcgagct cgattccgac gattcctact cgtattcgca tctcgcgaca
     4621 tcgctgtatg aacaggggca gttgcatgag gcagtgaagg ccttccaaca tgcggttgag
     4681 atcaaccccg aagactccta cgccttgtac aatctgggca tggcctacat ggatctgcat
     4741 gatgtcgacg cagccattga tgcattcgtg aaagtcgtga acctcgagcc gaacgatgcc
     4801 tacgcctgct tcaacctggg gttggcatac gaagaaaagc gcttgttgcc cgaagcgaaa
     4861 gcctgttttc agaaagcact gcagcatgct cagaaaggaa ctcaggttga gcggtttgcc
     4921 cgtgaatctc tccgtcggat gaaataaaca tgtctttccc ctcccactct cgacaccagg
     4981 ctgatggcaa gaacgggacc gttcgcgtct cgaatcgcaa ccagggtgca caggcgacgg
     5041 ccggtggact cacgctgaat gcggtgcggc acgcctcgca ggggcagggc aagcccggtg
     5101 gtcgagcgat gatgttggga atgatgagtg gttggttgag tgtcgtgctt ctgggagcct
     5161 tgctgatcat gggttgcgac actgacaaca acgtcaagtc tcgagaaggc gctctctcgg
     5221 ggaaagtggt cgacaaagcc ggcacggcac tgcagtatgc gctcgtgagc tgggctgaag
     5281 atcgcacccg gtggggtcgt accgatgcga acggcaagtt cgtggtcgag ggtgtgtcct
     5341 ttggcacccg ccagtttgtt gtggaactca ccggttaccg cacgcgaact ttcgacgtaa
     5401 acatttattc tgaagccatc a
//
LOCUS       DAORHD010000130         5389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15664_length_5389_cov_3.529246, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000130 DAORHD010000000
VERSION     DAORHD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15664_length_5389_cov_3.529246"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PKO06_04035"
     CDS             complement(<1..1756)
                     /locus_tag="PKO06_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HNV68843.1"
                     /translation="MSRAHEPLIRALQTVASWYSGILFSSRTVTGLLMMAAVTWNPDY
                     GVASLLCACLMLFWAKLFDIPETEVHAGGYFANAILFGLYLGNTFAHLPLLLLFSFSG
                     SFVVFILTRTLTQILWVRYGLPVCSYPFVLAVFLFTLLCRSEEVWFGTVFYRPWSYSG
                     GRVDLFFHQPFLLSVVDKLWHSVLGFVFSVGAILYQKQFVPCLLATIALVLSSRISTL
                     MAIAGYLCTRVLMLLFPFTCIGYETAFGFNAVLISIAIGSIYFVPGTKTTILMSSSQI
                     MGFLIGLFAIGSTRITGGDWTALPFNVTVTAILLAMQGRKPSARPMKPGLAFESPEDA
                     VVYYRRYEDRIFLRTVCLPVFGRWKIVQAFDGHETHREYWRYGVDFAAVDAAGSRFRT
                     SGITVDDYFAFQAPVLSPVDGVVAAVEAGVDDNALGHMNLAQPWGNGVIIFSAGLYIG
                     LYHLKKASILVVPGQVVGVGTPIGSVGNSGRSAVPHLHLQVQETPTLGAANLPFLLAN
                     VVLDESGRRCFKPFFRPDRIVSLEPMSGDDARRLPWLPRNGEIWHLELEEPHGKRMMT
                     WTCSYTLYGNVVVTAADGE"
     gene            complement(1789..3159)
                     /locus_tag="PKO06_04040"
     CDS             complement(1789..3159)
                     /locus_tag="PKO06_04040"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV68844.1"
                     /translation="MRREYQKPRIFPHTEGAMNKFGPATGSYKDAIDGARVAELVQKY
                     GSPLFVFSERTIRDTYRKAAGEFAHRYPQVQYSWSYKTNYLDAICQIFHQEGSWAEVV
                     SQFEYEKALRLGVPGEQIIFNGPYKPYEALRQAVKAKSKINVDNMEEIFNLEKLAVEL
                     RKPIDLGIRVNLDAGIYPAWTRFGFNLENDAAFSAVEKMHSTGKLRLKGLHCHLGTFI
                     LEPKAYRTAVQKLAQFARRLRDELAIKIEYLDLGGGFPSTNTLHTQYLPGNQTNPTVD
                     EFARVICDELLLRRDCFSTPPLLILETGRAMIDESGYLVASVVGSKRLTDGTPSLILD
                     AGVNLLFTSTWYKHNVIPTRPHSEFLENMILYGPLCMNIDVVRPMVQLPALAVGEQVL
                     IHPVGAYNVTQWMQFIQYRPAVVLVDEAGKPHEIRAKEDLDYMTRLESVPEHLRERKR
                     RVKHAR"
     gene            complement(3156..4316)
                     /locus_tag="PKO06_04045"
     CDS             complement(3156..4316)
                     /locus_tag="PKO06_04045"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV68845.1"
                     /translation="MKINLAVTGLNASDSPGPGLGIIKCLRAQTRFDVRIIGLAYDAK
                     EATIYVENACDEVYLIPYPSAGVEAFKSRMREVVQRAKVNFFLPALDSELANALAMEA
                     ELKSLGVRTFLPTAEMIEMRSKLKLQELCRKARLRYPQTIEISNYDQLFAGARRLGYP
                     CVVKGIFYEAVIARNDADLDKAARAISFRWGFPLLLQQHVPGEEFDIAIAGDGEGRMI
                     GITAMKKMALTDKGKAWGGMTIQSPGLFACCRRLMKHLEWRGPMEAEIMRCEKTDQYY
                     LIEINPRFPAWIYLSHGAGCNLPELLLQHLYEKNRSRSSTVFARPGTLFLRYAQDMII
                     SMPEYESMLMSGELRRGAPTVIESTARVTRRRSTPGTPAETSIRTRKKEARS"
     gene            complement(4306..4554)
                     /locus_tag="PKO06_04050"
     CDS             complement(4306..4554)
                     /locus_tag="PKO06_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HNV68846.1"
                     /translation="MGKKMADRFNILASGLIIDLVSGRTFSANPTGLVTFRGIMEGRK
                     KAQLVADLVKEFEIDEKTATRDVEEFLVELKSFHLHED"
     gene            complement(4725..>5389)
                     /locus_tag="PKO06_04055"
     CDS             complement(4725..>5389)
                     /locus_tag="PKO06_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68847.1"
                     /translation="LALTRVWQWVGIGLLVAVVAPWFWGAAEGLGRWFYLVQPLWGRA
                     LVENVPTDTAFPAFPWLVYPLTGLIIGLSMRSGTTENQLRPGMTAAGSVLLVSGLIIV
                     QIWGQTQYGDYYRMPVGGTFVYLGFALLWISSFLWLSKYGLGQKPLSALSFWSEHITL
                     VYCVQWFLFGFSVLFLRFRTVDSLLVLVALIPVFLGLTWAISRLLLRSARFMHVFRWF
                     SR"
BASE COUNT         1292 a         1607 c         1422 g         1068 t
ORIGIN      
        1 gttcaccgtc agccgcggtc acgacaacat tgccatacag cgtataagaa cacgtccagg
       61 tcatcattct cttgccatgt ggctcctcga gctcgaggtg ccagatctct ccgttccgcg
      121 gcaaccaggg caggcgtctc gcgtcatcgc ccgacatcgg ctcgagagac acaattcgat
      181 caggccgaaa gaagggcttg aagcatcgtc gacccgattc gtccagaacc acgttggcaa
      241 gcaggaacgg caggttcgca gcaccgaggg ttggcgtctc ctgtacctgc agatgcaaat
      301 gcggaaccgc gctgcgaccc gaattgccga cactgccaat cggggttccg accccgacaa
      361 cctgcccggg cacgaccaga atagatgcct tcttgaggtg atacagaccg atatacagcc
      421 cggctgaaaa gatgatgacg ccgttgcccc acggttgtgc cagattcatg tgtcccagcg
      481 cgttgtcgtc aacacctgct tctaccgccg cgaccacccc atcgaccggc gagagtaccg
      541 gtgcctgaaa ggcaaaatag tcgtcgaccg tgatgccgct cgtgcgaaag cgactgcctg
      601 ctgcatcgac cgcggcaaag tcaacaccat agcgccagta ttctcgatgg gtttcgtgac
      661 catcgaatgc ctgtacgatt ttccagcgcc caaagaccgg cagacatacg gtccgcagaa
      721 agatgcgatc ttcgtaccgt cggtaataga cgaccgcatc ttccggcgac tcgaacgcca
      781 accccggttt catcggcctg gcagacggtt tacgtccctg catcgccagc agaatggccg
      841 tgaccgtcac gttgaacggc agtgctgtcc agtctccacc ggtgatacgc gttgatccga
      901 tggcgaaaag gccaatgaga aaacccatga tctgagacga cgacatgaga atcgtcgtct
      961 tcgttcccgg cacgaaatag atgctgccga tggcgatact gataagaacc gcgttgaagc
     1021 cgaaggctgt ctcatacccg atgcaggtga acggaaacaa cagcatgagt actcgtgtac
     1081 agaggtatcc ggcaatcgcc atcagagttg agatccgtga ggacaacacc agggcgatcg
     1141 tcgccaggag acacgggaca aactgcttct gatacagaat tgccccgacg gagaatacga
     1201 aaccgagaac gctatgccag agcttgtcga cgacagacag cagaaacggc tggtgaaaga
     1261 acagatcgac ccgaccgccc gaatacgacc acggccgata aaacacggtt ccaaaccaca
     1321 cttcctcaga ccggcacagc agggtaaaca gaaagaccgc caacacgaat ggatacgagc
     1381 acaccggcag accgtatctc acccacagaa tctgtgtcag agtacgagtg agtatgaaga
     1441 caacaaagct tccagaaaaa gaaaagagca gaagcaatgg aagatgcgca aaggtattgc
     1501 cgagatacag accgaagaga attgcattgg cgaagtagcc cccggcatgc acctcggttt
     1561 ccggaatgtc gaaaagcttc gcccagaaca gcatcaaaca tgcacagagc agtgacgcca
     1621 caccatagtc gggattccag gtgacggctg ccatcatcag cagacccgta accgtacgcg
     1681 acgaaaagag aattccgctg taccagctgg ctaccgtctg tagtgcgcgt atcaagggtt
     1741 catgggctcg actcacggca cgatccttcc atggtgacag tcggctcgtc agcgagcgtg
     1801 cttcacccgt cgttttcgtt cacgcagatg ttccggcact gactcgagtc gggtcatgta
     1861 atcgagatct tctttggccc ttatttcatg aggttttccg gcttcgtcta ccaggacaac
     1921 cgcaggacga tactgaatga actgcatcca ctgcgtgacg ttataggctc cgaccgggtg
     1981 aatcagcacc tgctctccga cagcgagagc cggcagctga accatcggtc gtacgacatc
     2041 gatattcatg cagagcggac catagagaat catgttttcg agaaactctg aatgcgggcg
     2101 cgtcggtatg acattgtgct tgtaccaggt cgaagtgaac agcagattga ctccagcatc
     2161 aagaatcagc gagggagtcc catcggtgag acgcttcgat ccgacgacac tggccaccag
     2221 atacccggac tcatcgatca tggcccgacc ggtttcgaga atcagtaacg gcggggtcga
     2281 aaaacaatcg cggcgcagca gcagttcatc gcagatcacg cgagcaaatt catcaaccgt
     2341 cgggttcgtt tgatttcccg gcagatactg ggtatgcagc gtgttggtcg agggaaaacc
     2401 gccaccaaga tcgaggtatt cgatcttgat cgccaactcg tcacgcagcc ggcgcgcaaa
     2461 ctgagccagt ttctgaactg ccgtgcgata cgccttgggt tcgagaatga acgtgcccag
     2521 gtgacagtgc agtcccttca gccgcagttt gccggtcgaa tgcatcttct cgacggccga
     2581 aaacgcggca tcgttttcga gattgaaccc gaaccgcgtc caggccggat agatgccagc
     2641 gtccagattc acgcgaatgc cgagatcgat cggcttgcgc agctcgaccg ccagcttctc
     2701 gagattgaaa atctcttcca tgttatcgac attgatcttc gatttcgctt ttaccgcctg
     2761 tctcaaggct tcatacggct tgtacggtcc attgaagatg atctgttcgc caggcacccc
     2821 aagacgcagg gccttttcgt attcgaactg tgaaacgacc tcggcccaag acccttcctg
     2881 atgaaagatc tgacagatcg catcgagata gttggttttg tacgaccacg aatactgaac
     2941 ctgcgggtag cgatgagcga attcgccggc agctttgcga taggtatccc gaatcgttcg
     3001 ttcggaaaac acgaacaggg gcgacccata cttctgcacg agttcagcca cacgcgcgcc
     3061 atcgatcgcg tctttgtacg agccggttgc cggtccgaac ttgttcatag cgccttccgt
     3121 gtgaggaaaa atgcgcggtt tctgatactc tcgtctcacg accgggcctc cttctttcgt
     3181 gtgcgaatcg atgtctctgc cggtgttcct ggcgttgaac gacggcgcgt gacccgggcc
     3241 gtgctctcga tgaccgtcgg cgcccctcgg cgcagttcgc cagacatcag catgctctca
     3301 tactcgggca tcgagatgat catatcctgc gcgtaccgca gaaagagcgt tcctggccgg
     3361 gcaaacaccg tcgacgatcg cgaccggttc ttctcataca ggtgctgcag caacagctca
     3421 ggcagattgc agcccgcacc gtgactgagg tagatccagg caggaaatct cgggttgatc
     3481 tcgatcagat agtattgatc ggtcttctcg cagcgcatga tctccgcttc catcgggccg
     3541 cgccactcga gatgtttcat caaccgacgg cagcacgcaa acaggcccgg cgactgaatc
     3601 gtcatgcctc cccaggcctt gcccttatcg gtcagcgcca ttttcttcat cgcagtgatg
     3661 ccaatcatgc gcccttctcc gtcgcccgcg atcgcgatat cgaattcttc acctggtaca
     3721 tgctgctgca gcagtagcgg aaaaccccag cggaacgaga tcgcccgcgc cgctttgtcg
     3781 agatcggcgt cgttccgggc tatcaccgcc tcatagaaga tgcctttcac cacgcacggg
     3841 taccccagtc ggcgggctcc ggcaaacagc tggtcgtagt tcgagatctc gatcgtctgc
     3901 ggataccgca atcgagcttt gcgacacagt tcctgcagtt tcagcttcga acgcatctcg
     3961 atcatctcag cggttggcag gaatgtccgc acgcccagag acttcaactc tgcttccatg
     4021 gccagcgcat tcgccagttc cgaatcgagg gcgggaagaa agaaattcac cttcgctctc
     4081 tgcaccacct cacgcatgcg tgacttaaat gcctctactc ctgccgacgg atacggaatc
     4141 agatacactt cgtcgcaggc gttttctacg tagatggtgg cttccttggc gtcgtacgcc
     4201 aggccgataa tgcgtacgtc aaaccgggtc tgcgcccgca gacacttgat gatgcccaga
     4261 cccggaccgg ggctgtcaga ggcattcaag ccggtgacgg cgaggttaat cttcatgcag
     4321 atgaaatgat ttcagctcga ccagaaactc ttcgacatca cgcgtcgccg tcttctcgtc
     4381 gatctcgaat tccttgacca gatcggcgac taactgcgcc tttttccggc cctccatgat
     4441 accgcgaaag gtcacgagac cggtggggtt cgcagaaaac gtgcggccag acacaaggtc
     4501 aattatcagc cctgaagcca ggatgttaaa tcgatcggcc attttcttgc ccacgcgtcc
     4561 tccccgttcc tgaacctgga acgcctacag cacgttccga attttcagac aattccgagt
     4621 ctgtcgttcc acattcggaa cacctgactc gcgttcttct agggtaaccg agggaaggtg
     4681 ttttgcccac ttttttcgtg acgacggtgc cacgaaagct ctcatcatcg gctgaaccag
     4741 cgaaacacgt gcatgaatcg agccgaccgc aagagtagtc tggagatagc ccaggtcagg
     4801 cccagaaaca cgggaatcag ggccacgaga accagcagac tgtcgacggt acgaaacctc
     4861 agaaacagta cgctgaagcc gaatagaaac cattgaacgc aatagaccag ggtgatgtgt
     4921 tcactccaga acgagagagc tgacagcggc ttttgtccga gcccgtactt cgacaaccag
     4981 agaaacgaag agatccacag cagagcaaat ccgagataga cgaaggtgcc acccacgggc
     5041 atgcggtaat aatcaccata ctgagtctgc ccccagatct gaacgatgat caatcctgac
     5101 acgagaagaa cgctccctgc tgcagtcatt ccggggcgca gttggttctc ggtcgttcca
     5161 gagcgcatcg agagaccgat gatcaacccg gtcagtggat acaccagcca cgggaaagcc
     5221 gggaacgccg tgtctgtggg cacgttttcg accaatgccc gcccccagag aggttgcaca
     5281 agataaaacc accgccccag gccttccgcc gccccccaga accacggcgc caccacggcc
     5341 accagcagac cgattcccac ccactgccac actcgtgtca aagccagtt
//
LOCUS       DAORHD010000131         5381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15698_length_5381_cov_4.425648, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000131 DAORHD010000000
VERSION     DAORHD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15698_length_5381_cov_4.425648"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKO06_04060"
     CDS             <1..623
                     /locus_tag="PKO06_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68848.1"
                     /translation="RAFLQRAREEGRYVQAALTDANTWITLEPENGFACFHRASINLA
                     VGEKDAALQDLTTGLQKADPTGTKPNRRLAQAYIHRALLLLERKQYEPAEKDLRRAQE
                     LDDEDPDLSYARSFLAERDGNAREALHLMERADFLYAQRHKTSYLLEAPMVNNRYFKR
                     LYKLREACGFDPMKRSERFAESVEPVPAGGDDDDDDERLAQPLLPR"
     gene            586..4275
                     /locus_tag="PKO06_04065"
     CDS             586..4275
                     /locus_tag="PKO06_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV68849.1"
                     /translation="MMSVWRSRFCPGENVVPKPPSTIMRSGLFDTSAETVMNRLATAR
                     IPGPLAACLVSLWLACLTFPALVYAQSAPGASQPVILAGCEIDYPPFYLVTPDGQATG
                     FATELLRAAMRAVGRDVTYRTGPWNEVKGWLETGTVQVLPLVGRTPEREALFDFTFPY
                     LTLHGAIVVRKGNGGVQTLADLKGRSVAVMQSDNAEEFLRRNNPGCEIRVTRTFVDAL
                     RELEAGRHDAVVMQRLLAVRLIKELGLSGVEIVHTPITGFRQDFCFAVKEGDRDSLAL
                     LNEGLALVMADGTHRQLHARWMGNLDLPSHRRLIIGGDHRYPPWEFLDETGAPAGFNV
                     ELTRAIAQELGFDVEIRLGPWPEMVRLLESGEIDALQGMFYSLKRDAVFDFSNAHSVN
                     GCVVACRRDAGPPPASVAELRGRRITVENGDLTHEWALENGLASEVITVESQEAALRE
                     VRAGHSDCALVGRMTARYVIEKQGWNDLICGQKPIISPEYCYTFRNGQTALRTQFNEG
                     LSVLEQTGEYRRIQEKWFGVQERPHDSLSTLGRWLALASVPLLVIFLISAAWTWTLKR
                     QVAAKTYELRESEASHRRLFETMAQGVVYQNPDGHIVSINPAAEHILGLTRDQLLGLT
                     SMDPRWRTIREDGSELPGSEHPAMIALRTGKPVGPCVMGVARPDEPHHRWLRVNARPL
                     FRPGEDRPYQVYATLEDITELRRAREEYQQLFRLMLNGVVVAEVVRDEVGKVIDFRFL
                     AVNPAYERLVGLSAEKSIGRRVRELLPQLEDSWIEVYGRVVDTGEPVRFESEAKVLGK
                     HFAVSAFRTAPNQFASIFFDVTERRRAEEEREHLHEQLLQAQKMESVGRLAGGIAHDF
                     NNILGVILGRVELMMESMDEHHPQRHDLLEIEKAAQRSADLTRQLLAFARKQMAVPQV
                     INLNSVIDGLLRMLARLLGENIRIDWKPASDLWPIEIDPVQIDQVLVNLSVNARDAIK
                     DAGTIGITVENAVVTHDTSDRMLGVAPGEYVHLRFADSGCGMRQHVKDKLFEPFFTTK
                     DVGKGTGLGLATVYGVMKQNGGYIDVQSEVGKGTIFHLYFPRHIESTNASEKDDVSQT
                     TAADARVARHQQATILLCEDHADIRELTRLMLEKRGFTVLVASGPTEALELARAHRKN
                     IRLVITDIVMPEMNGRELTRQLTGLIPELKCLYMSGYTADVILEHGVAEDGLTFIQKP
                     FTSARLFEKIDALLDATSAVSGESP"
     gene            complement(4354..>5381)
                     /locus_tag="PKO06_04070"
     CDS             complement(4354..>5381)
                     /locus_tag="PKO06_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV68850.1"
                     /translation="PFAAVVADYQMPEVNGIDFLKRVQKPSPETTRMMLTGQTELNVA
                     MQAVNEGHIFRFLTKPCPYDVLTKALDDAFRQYQLVTAEKELLEKTLKGSIKILVDLL
                     ALARPELFMRTTALSPLVRRMTDRLQGNQQITELALMFSRLCHLILPPSVLADVQKGA
                     APADANVQRMIDRQPLVIQRILLQIPRLEEIAKVVGYLHKNYDGGGEPSDDVSGENIP
                     LGSRLLRAVLDFDDLRGRGQNEAQAFHSLTARIGTYDPNVLKALKAEIELSHEGLDLI
                     EVNVEDLAPGMIMADNVVTVGGTTLLREGIELTELFRTRIVQYDRIDRVVIPIRVWKA
                     REPSRKS"
BASE COUNT         1114 a         1542 c         1644 g         1081 t
ORIGIN      
        1 ggcgggcgtt tctgcaacga gcccgtgaag agggaagata cgtgcaggcc gctctcaccg
       61 acgcgaatac ctggatcacg ctcgagcccg agaatgggtt cgcctgcttt catcgcgcgt
      121 cgatcaatct ggcggtcggt gaaaaagacg cagccctgca ggacctcacg acaggcctgc
      181 aaaaggctga cccgactggt acgaaaccga accgcagact ggcgcaggcc tatattcacc
      241 gggcgcttct ccttctcgaa cgcaaacagt acgagcccgc cgaaaaagac ctgcggcgcg
      301 cccaggaact cgatgatgaa gatcctgatc tgtcctatgc ccgcagcttt ctggcggaac
      361 gcgacgggaa cgccagggaa gcgctgcacc tgatggaacg cgcggacttt ctgtatgcgc
      421 agcgacacaa aacctcgtat ctgctcgaag cgccaatggt caataatcgc tacttcaagc
      481 gtttgtacaa attgcgtgag gcgtgcggtt tcgatccgat gaaacgatcg gaacgattcg
      541 ccgagtccgt cgaaccggta ccagccggcg gtgatgatga tgacgatgat gagcgtctgg
      601 cgcagccgct tctgccccgg tgagaacgtg gtgccaaaac caccatcgac gatcatgaga
      661 agcgggctat tcgacacctc agccgaaacg gtcatgaacc gactcgcgac tgcacgcatt
      721 cccggtccgc tggccgcgtg tctggtcagc ttgtggctgg cctgtctgac ttttcccgcg
      781 ctcgtctacg cgcaatccgc gccgggcgct tcccagcccg tcattctggc cgggtgcgaa
      841 atcgattatc ccccattcta cctggtcacg cccgacggcc aggcgacggg tttcgcgacg
      901 gaactgctgc gggcggccat gcgagccgtc ggacgtgatg tcacgtatcg caccgggccc
      961 tggaacgagg tcaaaggctg gcttgagacc ggaaccgtac aggtcttgcc cctggtgggg
     1021 cgcacacctg agcgcgaagc gctgttcgac ttcaccttcc cctatctgac gctgcacggg
     1081 gcgatcgtcg tccgcaaggg aaacggcggg gtccagacgt tggcagatct gaaagggcgt
     1141 tccgtcgctg tgatgcagag tgacaatgcc gaggagtttc tgcggcgcaa caaccccggc
     1201 tgcgagattc gcgtgaccag aacctttgtc gacgcgctgc gtgaactcga ggccggcagg
     1261 cacgacgcgg tcgtcatgca gcgattgctg gcggtgcgcc tgatcaagga attgggattg
     1321 tccggggtgg aaatcgttca caccccgatt accggctttc ggcaggattt ctgttttgcc
     1381 gtgaaggaag gagatcgcga ctcgcttgcc ctgctcaacg agggcctggc gctggtgatg
     1441 gccgacggca cgcatcgtca gctgcatgcg cgctggatgg gcaatctcga tctgccatcg
     1501 catcgacgac tgatcatcgg cggcgatcac cgctacccgc cgtgggagtt cctcgacgaa
     1561 accggtgccc ccgcggggtt taacgtcgaa ctgacccggg ccattgcgca ggaactcggc
     1621 ttcgatgtcg agattcgtct cggaccctgg cccgagatgg tgcgcctcct cgaaagtggt
     1681 gagatcgatg cgttgcaggg catgttttac agcctgaaac gcgacgcggt gtttgatttc
     1741 tcgaacgcgc attccgtcaa tgggtgcgtg gtcgcctgcc gacgcgatgc gggccccccc
     1801 ccggcctcgg ttgccgagct tcgtggccgt cgcatcaccg tcgaaaatgg cgatctgacg
     1861 cacgagtggg ccctcgaaaa cggcctcgcg agcgaagtga tcacggtcga gtctcaggaa
     1921 gccgccttgc gcgaagttcg agccggccac agtgattgcg ccctggtcgg tcgaatgacc
     1981 gcccggtatg tgatcgagaa acaggggtgg aacgacctga tctgtgggca gaagccgatc
     2041 atttcgccgg aatactgcta cacgtttcgc aacggacaaa ccgccttgcg aacccagttc
     2101 aacgaagggc tcagcgtgct cgaacagacc ggcgagtatc ggcgtattca ggaaaagtgg
     2161 ttcggcgtgc aggagcggcc gcacgattcg ctgtcgaccc tgggacgttg gctggccttg
     2221 gcgtcggtgc cgctgctggt gatattcctt atctcggctg cctggacatg gacgctgaaa
     2281 cgtcaggtcg cggcgaaaac gtacgagtta cgggaaagtg aggccagcca tcggcgcttg
     2341 ttcgagacga tggctcaggg ggtcgtgtat cagaatcctg acggtcatat cgtctcgatc
     2401 aacccggcgg ccgaacacat tctcggattg acccgcgatc agcttctcgg gcttacctcc
     2461 atggacccga gatggcgaac gatccgggaa gatggttctg aactgcccgg ttcggaacac
     2521 ccggcgatga ttgccttgag aaccggaaag ccggtcgggc catgcgtgat gggggtggct
     2581 cgccctgatg agccgcacca ccgctggctg cgggtgaacg cccgcccgct ctttcgaccc
     2641 ggcgaagaca ggccatacca ggtgtatgcg acgctcgaag acattaccga actacgccgg
     2701 gcgcgcgaag agtaccagca gctctttcgc ctgatgctca atggggtcgt ggtggcagag
     2761 gtggttcgag acgaggttgg gaaggtgatc gatttccgct ttctggcggt gaacccggcg
     2821 tatgaacgtt tggttgggct gagtgccgag aaaagcatcg gtcgccgcgt gcgagagttg
     2881 ttgcctcagc tcgaagattc ctggatcgag gtctatggac gcgtggttga cactggtgaa
     2941 ccggtacgat tcgagagtga agccaaagtg ctcggcaagc acttcgcggt gagtgcgttt
     3001 cgcaccgctc caaaccagtt cgcgtcgatt ttcttcgatg tcaccgagcg ccggcgcgcc
     3061 gaagaagaac gagaacatct gcacgagcag ctcctgcagg cgcagaagat ggaatcagtc
     3121 ggtcggcttg ccggtggtat cgcgcacgac ttcaacaaca ttctcggcgt cattctcggc
     3181 cgcgtcgaac tgatgatgga atccatggat gaacatcacc cacagcgcca cgacctgctc
     3241 gaaattgaga aagcggcaca acggtcagcg gatctgacca ggcagttgct cgcgtttgcg
     3301 cgcaaacaga tggccgtgcc gcaggtgatc aacctgaaca gcgtcatcga tgggttgctg
     3361 cgcatgctgg cgcgtctgct gggcgaaaac attcgtatcg attggaaacc cgcatctgat
     3421 ctgtggccga tcgaaatcga cccggtacag atcgatcagg ttctggtgaa cctttccgtc
     3481 aatgcccgag atgccatcaa agacgccgga accatcggca ttaccgtcga gaacgctgtc
     3541 gtgactcacg atacctctga tcgaatgctg ggcgtggcac ccggagagta tgttcatctg
     3601 cgctttgccg acagcggctg cggcatgcgc cagcacgtca aggacaagct gttcgaaccg
     3661 tttttcacca ccaaggatgt cggcaaggga actggccttg gcttggccac cgtctacggc
     3721 gtcatgaaac agaacggcgg ctacatcgac gtgcagagcg aggtgggaaa aggcacgatc
     3781 tttcacttgt acttccctcg acacatcgag tcaacgaacg cgagcgagaa agacgatgtc
     3841 agccagacca cggcggccga cgcacgtgtc gctcgacacc agcaggccac gatactgctc
     3901 tgcgaagacc atgccgacat tcgtgagctg acacgcctga tgcttgagaa gagagggttc
     3961 accgtcctgg tcgccagcgg accaaccgaa gcactggagc ttgcgcgcgc gcatcggaag
     4021 aacattcgac tggtgatcac cgacatcgtc atgccggaga tgaacggtcg tgagctgaca
     4081 cggcagctga ccgggctgat tcccgagctt aagtgcttgt acatgtccgg ttacaccgcc
     4141 gatgtcattc tggaacacgg ggtggcagaa gacggcctca ccttcattca aaaaccgttt
     4201 acctcggcgc gtttgttcga aaagatcgat gccttgctcg atgcgacttc tgcggtgtcc
     4261 ggagaatctc cgtgaggtgc gttggaaccc agatgcatcg aggaatggtg ccgtccgtac
     4321 attgaggccg agccagaacc ggtgatctga ccttcacgat ttcctcgatg gttcgcgagc
     4381 tttccagacg cgtatgggta tgaccacgcg atcgatgcgg tcatactgca cgatgcgggt
     4441 cctgaacaat tcggtcagtt cgatgccttc acgcagcaga gttgtgccgc cgacggtgac
     4501 gacgttgtcg gccatgatca ttcctggcgc gagatcttcg acattgactt cgatcaaatc
     4561 gagcccttcg tgggaaagtt cgatctcggc cttgagagct ttcagaacat tcgggtcgta
     4621 ggtgccgatg cgtgcggtca gagaatgaaa agcctgggct tcgttctgcc cccgaccgcg
     4681 cagatcatcg aaatcaagaa cggctcgcag caggcgactg ccgagaggaa tgttctcacc
     4741 cgagacgtcg tcactcggtt ctccccctcc gtcataattc ttgtgaagat accccacgac
     4801 tttggcgatt tcttccagtc ggggaatttg cagtaatatt ctttgtatga ccaacggctg
     4861 ccggtcgatc attctctgga cattggcgtc tgctggagcc gctcccttct gaacatccgc
     4921 aagcactgac ggtggcaaga tcaaatggca gagtcgcgaa aacatcaacg ccaactccgt
     4981 gatctgttga ttgccctgca gacggtcggt catccgtcga accaacggag acaaggcggt
     5041 ggtgcgcatg aagagttcgg gtcgggcgag ggcgagcagg tcgacgagaa tcttgatcga
     5101 acccttcaag gtcttttcga gaagctcctt ttcggcagtc accaactggt actgacgaaa
     5161 agcgtcgtca agcgctttgg tcagtacgtc ataggggcag ggtttggtca gaaagcgaaa
     5221 gatatgtcct tcattcaccg cctgcatggc gacgttcagc tcggtctgcc ccgtcagcat
     5281 catgcgggtg gtctccgggg agggcttctg gactcgcttg agaaaatcga ttccgttgac
     5341 ttccggcatc tgatagtcag caaccacggc ggcaaatgga c
//
LOCUS       DAORHD010000132         5373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15730_length_5373_cov_3.724709, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000132 DAORHD010000000
VERSION     DAORHD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15730_length_5373_cov_3.724709"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..369)
                     /locus_tag="PKO06_04075"
     CDS             complement(151..369)
                     /locus_tag="PKO06_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68851.1"
                     /translation="MEPSPKTVINSTLMLWCGVVIAELFIIAHLMNSGLVLVFGLVLI
                     FPLRAAWNWPAPPEAPAVPAPASAPAVK"
     gene            complement(386..1750)
                     /locus_tag="PKO06_04080"
     CDS             complement(386..1750)
                     /locus_tag="PKO06_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68852.1"
                     /translation="MSASRDQIRGVLADLVAKQDKVLADAPGLEKLLQAKFVGMYSQE
                     IAAVKAALIEKVPWNLRRNNETVASRRMIQDLGSGVARAHAVSLDLACWAVESWALSL
                     RMTLETLTKAAPATPASASIPTSTPTPKMGANAVSAPQAAPLATAMVSTSTDGQAAAS
                     TVPARITPTTRMGLIYGVDDRGCMRVYQVWWNPVGGNEAASALASQVKPEPQRLVHLF
                     EAASVNKSVEATVTSDRLGHIPAGTPKNTTTPAPRSAASVAAGGAPALPPKAPVSTPV
                     PGAVRSTAIPPSAPTPRASTPQPTPVRPVAPAPVKPPQPVAAPPAAPSLPPRIPASRG
                     EELYDQAMAILNGSKAVARSGEVIQLLQQSAGQGYVLAETRLGELCMRGRVVKEDLPT
                     AVRWLQSAAQKGEPEAQTVLGSLYQCGMGVPLDLNEAQKWLQRAAKQGNAEAQQLLKQ
                     ILET"
     gene            complement(1770..2846)
                     /locus_tag="PKO06_04085"
     CDS             complement(1770..2846)
                     /locus_tag="PKO06_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV68853.1"
                     /translation="MNLRRAFKQIPGRTPIEHSFQRLHMRISVLVLLVTLLTTLVGCG
                     SGSVEFGRPALVVTDPDESRVVVYDNSPRRILILDSQFKLIREITHPLMQNVWGLTLG
                     LQGEILAATECADSSGLQEVAASSSAVAAILRFDRDGRELPPWIWRGRQGPLIKPIQI
                     TALPDATYLVTDAGVDRIFRLNDAGVVIGASGQNGSAPGQVYFPSDIKRLPSGKVLLT
                     DAFNSRVQEFRLNELATQSAPASGTLPEILTFERVVLENGMEPGKVRFPQYLAVAPSG
                     NWYVSEFETMRVSEFDQNGTLVRLFNPTLSDAATGTETFFELFGVAWLPKSKRLLVAD
                     SLNAGIHVFAEDGRVTEFLRGPKP"
     gene            complement(2896..3354)
                     /locus_tag="PKO06_04090"
     CDS             complement(2896..3354)
                     /locus_tag="PKO06_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNV68854.1"
                     /translation="MIIDTLARADWYLAINSRIARAFRYLQQTDLAALAAGTYLIDDR
                     RVYAIVQEYETRAESDAQYESHRRYLDVQYVISGHERIGWANRDLLAPGYYDEEKDLE
                     FHVGTGQLLDVPRGSFMLLAPHDAHLPCVHPLTGRTRVKKVVVKVLVRDD"
     gene            3503..5125
                     /locus_tag="PKO06_04095"
     CDS             3503..5125
                     /locus_tag="PKO06_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNV68855.1"
                     /translation="MAILYVDGKRLKRQIRASARWLVAHEDALNRLNVFPVPDGDTGT
                     NMGHTLKAVVRAISDSNLGNDLSDIASTVANSALLGARGNSGVILSQIFHGFAEGIGD
                     RKRANAMDIAYSLKRACEKAYAAVSSPTEGTILTVVREGVDAAYEKARTEVDIAVVIE
                     TLLTACKRSLANTPNLLPALKAANVVDAGAMGFVFIIEGIQRLLTGAELPETELEAGK
                     PDHSDPPGPASIDLRSQYGYCTELLIRGSRLAEAEIRNALQNLGDSLIVAGGANGVKI
                     HVHTARPGAILECCQHFGILHDVKIENMDAQHAALSAGEPEKQIAIIAVAMGEGLRRI
                     FESMGCDFVIQGGQTMNPSVQELSDAIRVTANRYRCRQLILLPNNGNVLFSAEQACAL
                     HAQREVVVIPTRTVPEGFSALMAFDPEADLPVNVERMRQAIKQVKTGEVTQAVKEYQS
                     DDLQVRAGDTIAIHQNKILGTFPDAQSAVRHMVATLLGESDAVISLFHGEDTPEVVAQ
                     ALAQELALTYPDLDVELHHGGQPHYAYIVSIE"
     gene            5137..>5373
                     /locus_tag="PKO06_04100"
     CDS             5137..>5373
                     /locus_tag="PKO06_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNV68856.1"
                     /translation="MPDRLVHIVTDSTCDLDQPVLQELGVHMVPLNVLFGHETFKDRR
                     ELPPTGFYAKLRSGAPHPTTSQPSPAEFRVVYEQL"
BASE COUNT         1113 a         1637 c         1600 g         1023 t
ORIGIN      
        1 tccccgcccc gactgcagca cgcgatacgc ccatttgcgc tgcagcagaa ggtccgagaa
       61 ccgcagatca ttctcggggg tgtagaacga cactccggtg gcgacgagaa attcgatttt
      121 ttccatgttc cggctccagg accgatcaga ttacttcaca gcgggggcgc tggcgggggc
      181 gggcacggca ggggcttccg gcggcgcggg ccaattccag gcggcacgca gcggaaagat
      241 cagaaccagg ccgaatacca gtaccagccc ggagttcatg agatgcgcaa tgatgaacag
      301 ttcggcgatg accacgccgc accagagcat aagggtcgag ttgatcacag tcttcggaga
      361 tggttccatg gtcgggctcc ctggttcagg tttcaagaat ctgtttcagc agctgctggg
      421 cttcggcatt cccctgcttc gcggcgcgct gcagccactt ctgcgcctcg ttcagatcga
      481 gcggtacacc catgccgcac tgatagaggc tgccgagcac cgtctgggcc tcgggttccc
      541 ccttctgcgc cgctgactgc agccagcgca ccgcggtcgg caggtcttct ttcaccacgc
      601 gaccacgcat gcagagctcg cccagtcggg tttccgccag gacatacccc tgacccgccg
      661 actgctgcag cagttgaatg acctcaccgg agcgtgcgac cgctttcgat ccgttcaaga
      721 tcgccatggc ctgatcgtag agttcttcgc cgcggctggc gggaatgcgc ggcggcagac
      781 tcggagcggc gggcggtgcg gcgaccggct gcggtggttt gaccggcgcg ggcgcgaccg
      841 ggcggacagg cgtgggctgc ggtgtcgaag cacgcggcgt cggagcagac gggggaatgg
      901 cggttgatcg gaccgcgcca ggcaccggcg tggataccgg agctttgggc ggcagcgcgg
      961 gagcgccgcc ggcagcaacc gacgcagccg atcgtggagc aggagtggtg gtattcttcg
     1021 gcgtacccgc gggtatatgt ccgagtcggt cggatgtcac agtcgcttca actgatttgt
     1081 ttacagaggc ggcctcgaag agatgcacca gacgctgcgg ttcgggcttt acctgactgg
     1141 cgagtgccga tgcggcttca ttgccaccga cggggttcca ccagacctga tacacacgca
     1201 tgcaaccacg atcatcaacc ccgtagatca gccccatgcg agtcgttggc gtaatgcggg
     1261 ctggtactgt tgaggccgcg gcctgtccat ccgtcgacgt cgacaccata gccgttgcca
     1321 gcggcgctgc ctggggagca gaaaccgcgt ttgctcccat cttcggcgtc ggagtcgatg
     1381 tcggaatcga tgctgaggcc ggggtcgcag gtgccgcttt cgtcagcgtt tcgagcgtca
     1441 tacggagcga caacgcccac gactcgacgg cccagcaggc cagatcgagt gacacggcat
     1501 gggctcgcgc caccccactg ccaagatctt gaatcatgcg gcgactcgcg acggtttcat
     1561 tgttgcgtcg caggttccag ggaactttct cgatcaacgc cgctttcacg gccgcgattt
     1621 cctgactgta catgccgaca aacttcgcct gcaggagttt ttccaacccc ggggcatcgg
     1681 ccagcacctt gtcctgcttc gccaccaggt cggccagcac tccgcgaatc tggtctcgag
     1741 aggcgctcat gccatcgatc tccaaccgtt cacggcttcg gtccgcgcag aaattcggtc
     1801 acccggccat cttcggcaaa aacatgaatg ccggcattga gagagtcggc gaccagcagt
     1861 cgcttcgact tcggcaacca ggccacgccg aacaactcga aaaacgtttc cgtaccagtt
     1921 gccgcatcgc ttaacgtggg attgaagaga cggaccagtg ttccgttctg atcgaactcg
     1981 ctgacccgca tggtttcgaa ctcggaaacg taccagttgc cgctcggggc gaccgccaga
     2041 tattggggga aacgcacctt tccaggttcc atgccatttt caagcaccac gcgttcgaag
     2101 gtcagaatct cgggaagcgt tcccgaggca ggggcgctct gcgtggccaa ttcgttcaac
     2161 cgaaattcct gcactcggct gttgaacgca tcggtcagca gcaccttgcc ggacggcaac
     2221 cgtttgatgt cgctgggaaa gtagacctgc cccggcgcac tgccgttctg acccgaggca
     2281 ccgataacca ccccggcgtc gttcagccgg aagatacgat cgacccccgc gtcggtcacc
     2341 aggtaggtcg catcgggcag agccgtgatc tgaatcggct tgatcagcgg cccctgtctg
     2401 cctcgccaga tccagggggg gagctcccgc ccgtcgcggt caaaacgcag aattgcggcc
     2461 accgctgacg atgacgcggc aacttcctga agaccagaac tatcggcaca ttccgtggcg
     2521 gccagaattt cgccctgcag acccagcgtc aggccccaga cgttctgcat cagcggatga
     2581 gtaatctctc gtatcagttt gaactgcgaa tcgagaatca gaatgcgacg cggactgttg
     2641 tcatacacga ccacccgtga ttcgtccgga tccgtgacga ccagtgccgg acggccgaat
     2701 tcgaccgatc cggagccgca accaacgaga gtggtgagaa gcgtaacgag caggaccaag
     2761 acactgatac gcatgtgcag cctctgaaaa gagtgttcta tcggcgtgcg tcccggtatc
     2821 tgcttgaacg cgcgacgcag gttcatggta ttactcgccc cggcggtcgt caacgattct
     2881 cttacgccga tacgctcagt catcgcgcac cagaaccttc acgaccacct ttttcacccg
     2941 agtccgcccg gtcagcggat gaacacaggg aagatgcgca tcgtgaggag ccagcagcat
     3001 gaacgaaccc ctgggaacat cgagaagctg cccggtccct acatggaact cgagatcttt
     3061 ctcctcgtcg tagtaaccgg gagccaacag gtcgcgattg gcccaaccga tgcgctcatg
     3121 cccggagatc acatactgaa catcgaggta acgccgatga ctctcatact gagcgtctga
     3181 ttccgcgcgg gtttcgtatt cctgaacgat ggcatagacg cgccggtcgt cgatgagata
     3241 cgtaccagcg gccagagcgg caagatcggt ctgctgcaga taccgaaacg cgcgtgcaat
     3301 gcgggagttg atcgccagat accagtcagc tcgagccagc gtgtcaatga tcatggagct
     3361 tgctccgaaa attgggtctg gcaagagtat atcagaatac gaaaacctca tcttcaccac
     3421 actggtcaaa aatgactgtg gggaatcagt tgcccggaag ggcgagtact ggtagaaatg
     3481 tggcgcgaga ggaaacaact tcatggcaat tctctatgtg gacgggaagc ggctgaagcg
     3541 ccagatacgt gcgtcggcac gctggttggt cgcccacgaa gacgctctca accgcctcaa
     3601 cgtgttcccc gttccggacg gggacaccgg caccaacatg gggcacaccc tgaaagcggt
     3661 cgtgcgggcc atttccgatt cgaacctggg aaacgatctg tcagacatcg cctcgaccgt
     3721 ggctaatagc gctctgctgg gcgctcgcgg gaactccggc gtcattctgt ctcagatctt
     3781 tcatggattc gcggaaggca tcggagaccg caaacgggcc aacgccatgg atatcgccta
     3841 ttcgctgaag cgggcctgtg aaaaggccta cgccgcggtc agcagcccga cggaaggcac
     3901 gattctgacc gtggtgcgcg agggagtaga cgcggcctat gagaaagccc gcaccgaggt
     3961 cgacattgcc gtggtcatcg aaactctgct tacggcctgc aaaaggtcgc ttgccaacac
     4021 cccgaacctt ttacctgcgc tgaaagcagc caacgtcgtt gacgcgggcg ctatgggatt
     4081 cgtcttcatc atcgagggca ttcagcgact gctgaccggg gccgaactgc ccgaaaccga
     4141 gctggaagca ggcaaaccgg atcattcaga ccccccaggt ccggcgtcga tcgaccttcg
     4201 ctcacagtac ggctactgca cggaactgct gattcggggg tcacgactgg cggaagcgga
     4261 gattcgcaac gctctgcaga acctcggcga ctccttgatc gttgccggtg gagcgaacgg
     4321 tgtcaaaatt catgttcata ccgcacgacc gggtgcgatt ctcgagtgct gtcaacactt
     4381 tgggattctg cacgacgtca agatcgagaa catggacgcg cagcacgccg cgctttccgc
     4441 gggagaaccc gagaagcaga tcgcgatcat cgcggtcgcc atgggtgagg gtctgcgacg
     4501 catcttcgag tcgatgggct gtgatttcgt cattcagggc gggcagacga tgaacccgag
     4561 cgttcaggag ctcagcgacg cgattcgtgt cactgccaac cggtaccgtt gtcgacaact
     4621 cattctgctg cccaacaacg gcaatgtctt gttttctgcc gaacaggcct gcgccctgca
     4681 cgcgcaacga gaagttgtgg tcatcccaac ccggacggtt ccggaagggt tttcagcgct
     4741 gatggctttt gatcccgagg cggatctacc cgtaaacgtc gaacgaatga ggcaggcaat
     4801 caagcaggtc aaaaccggcg aggtgaccca ggccgtgaaa gagtaccagt ctgatgatct
     4861 gcaggtgcga gccggagaca cgattgccat tcatcagaac aagattctcg gcacttttcc
     4921 cgacgcgcag agcgcggtga ggcacatggt cgccactctg ctcggcgaga gtgacgccgt
     4981 catttccctg tttcatggtg aagacacgcc ggaagtcgtc gcgcaagccc tggcccagga
     5041 actggcgctc acgtatcctg acctggacgt cgaactgcac cacggcggac aacctcatta
     5101 cgcctacatc gtttcgattg aatagaagca cttttcatgc cagatcgact tgtacacatc
     5161 gtcaccgaca gcacctgtga tctcgatcaa ccggtgcttc aggaactcgg ggtgcacatg
     5221 gtaccgttga acgtcctgtt cggtcacgaa acgttcaagg accgccgcga gttaccgcct
     5281 acgggttttt atgccaagct gcgttccgga gccccccacc ccactacttc gcagccctcg
     5341 ccggccgagt ttcgcgttgt ctacgagcag ctg
//
LOCUS       DAORHD010000133         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_15924_length_5333_cov_3.417772, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000133 DAORHD010000000
VERSION     DAORHD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15924_length_5333_cov_3.417772"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKO06_04105"
     CDS             <1..449
                     /locus_tag="PKO06_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68857.1"
                     /translation="LAIGPALYLAKTAQKQELLSEKEFTATLLAQHALERVIAYGRVR
                     PGALPPMTEEEPVVVSPDSPRQVSWYFLDLFGHVAGISETDDQALYWHLKPFTCEVKT
                     YLIEQGVFKVIVYIGYLDGGRQKKVFLERLFDIPQQLDREPQGATP"
     gene            446..886
                     /locus_tag="PKO06_04110"
     CDS             446..886
                     /locus_tag="PKO06_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68858.1"
                     /translation="MKTGFITLPVVLVVIGLLLAGSLFLLIPNPHSSPRDLLPEVQTA
                     MDNYQTFLEQFNRDGKYASRIKVTEKELSFISSDNSEIFYRFTDGNLTREHRGQTSTL
                     LSNLGTVRFSGGQLLPQFVSVFIAYADNQLPSFFTSVAPRGVRP"
     gene            883..1407
                     /locus_tag="PKO06_04115"
     CDS             883..1407
                     /locus_tag="PKO06_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68859.1"
                     /translation="MMNNRGIAWLLLVAFIFLLAFIGGAGVYWRLNSNFVKPPLSTKL
                     QIDYQLESALLLVFQTIAAAGPEPLPATFTYEPPRREIMPGVVLSVRTVQRTPEEVQL
                     EAQAVGSRLERYLSARLFRGIASGPEMAERSALLPALDAASASADLSSPSTDLTTGSP
                     TWLIELLQRRTDTP"
     gene            1404..3593
                     /locus_tag="PKO06_04120"
     CDS             1404..3593
                     /locus_tag="PKO06_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68860.1"
                     /translation="MSKSARALPVSGHPRAGPAESLLMQGSFTHQRLSPTRTTRGFLL
                     MIVLVIGTILLLLGITLHFAMGRQNANLHWLANGEIAHFLADAGINSCIRAVRESVQT
                     SGLLNQGSSTNPLVQILTRPQPLDDTSLMSYLKDTWNAELKAFAAEVDPTASIRVEVW
                     LRDFRQTETDPAAWVDPVAKRGYLSIEATGEYRGVKRTLTVKRQIRVASCLPPLVSKF
                     TLHVKNAGDGGEGKFNIIRNDYNGNLTDGPRPFICYNHATPDAPFEARPHDPAGEADA
                     QVFEKRGWIWLGNQRVRLNLTSGPGLFGEIFHFYEVKDPSRFQAVGFVTPVDRLPAPF
                     SSPQTLPRDNFQAGQMAEYTFGHSFIFDGFHDKSNRRDTEAMYEGSILSNAEKSAFGA
                     KSSLLHLYGEARPGFMSRTRVLGNVQVALPRFASLDITPSDSDLRTRFSGQTPPPTYL
                     LPSVPQSSFDPNRQIKDFLGRRFGGPVMINALLFGTQYEEYKKFMSGIIELPYVTGYN
                     SMQDYHVDPTKRQFPPKHQILSEDRGLSLELKRGSDVVYKGSVDAQRLDDVVEGRVQK
                     VVPTISEFWKTYLDRTSGELHLNGIVAIENPDRQNLILPPSDKSSPLKVRGGGLILLE
                     QGNVTLRGVQLLSSSEALTIIAPEANSVRVVNAISQQVNVVAPMAEFVCDDKLDWFGT
                     LSVSSIQANPRNPGGVLRFRETQDPTRPESLSFAKIHIDESDSFWHE"
     gene            3586..4029
                     /locus_tag="PKO06_04125"
     CDS             3586..4029
                     /locus_tag="PKO06_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV68861.1"
                     /translation="MSNSRCGMSLVEILVAAGVLSLILLPVALSFSSGARGIQMTHEE
                     MTAHQAAIELMEQLQSLPFALVPAGTWVNDQIRGGMRLVQNSPLTFQVTPLDGFERGL
                     SITELSKDGRVRFKKIDISVSWKGQDGKGPARSFRVKGLLANETL"
     gene            4016..4615
                     /locus_tag="PKO06_04130"
     CDS             4016..4615
                     /locus_tag="PKO06_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68862.1"
                     /translation="MRPCNLATWSRRAGITLVEILIVVVIAVLLIGTLTTLFSGFRRS
                     FAKGEESTVVIQEGGFFLAHLRNDLINAVLPPGFDQADWQKAIVADADSLSFSLYADA
                     QGTIETVTYMTDGKSLKRMVKNGSPKPLIEGRIASLSWKTGWEVIPTPGSGTRRLWVE
                     LSATLGGQGKPGMTSKQLHLRTRLFPARLNRQLNGNLSP"
     gene            4612..>5333
                     /locus_tag="PKO06_04135"
     CDS             4612..>5333
                     /locus_tag="PKO06_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68863.1"
                     /translation="MKAIIRLLLPLLLPILISVLPFIILRDERNRALQLAGERLKRSW
                     HRQAEFIAQQFRSNCSIEKQIQNQTGTLKHLLQQAARRQLETRHLLTSQHFLRSYHQA
                     FPPRRHPNGRVFWNPASESLLLAFIPDGDSRVKALPTSSLPSYRLRPFTRLMNEMLQA
                     PRLSKQELTSKDSISKAVFGAYCQFEMIALTQKGIPTPVHFMDQRSFIVWDTIEVNGK
                     RMGAFLLIFSESIETRGRPLRHA"
BASE COUNT         1095 a         1667 c         1422 g         1149 t
ORIGIN      
        1 ccctcgccat cggtccagcc ctttatctgg ccaaaaccgc gcaaaagcag gaactcctct
       61 ccgagaaaga gttcaccgcc accctgctcg cccagcacgc cctcgaacgc gtgattgcct
      121 acggccgggt cagacccggt gctctccccc ccatgacgga agaagaacct gtcgtcgttt
      181 ctcccgattc gccgcgtcag gtttcgtggt attttctcga cctgttcggc catgttgccg
      241 gcatttcgga aaccgatgat caagccctct actggcatct gaagccgttc acctgcgagg
      301 tcaagacgta tctgatcgaa cagggcgtct tcaaagtgat cgtctacatc ggctatctcg
      361 acggtgggcg tcagaaaaaa gtctttctcg aacgtctctt cgacatccct cagcagctcg
      421 accgtgagcc tcagggggcc accccatgaa aaccggtttc atcacgcttc cggtcgtgct
      481 ggtcgtcatc ggcctgctgc tggcaggttc tctgtttctg ctgatcccga atccgcactc
      541 ttcgcctcgc gatctgctcc cggaagttca gacagccatg gacaattatc aaacgttcct
      601 tgagcagttc aaccgagacg ggaaatatgc gagtcgcatc aaggtgaccg agaaggaatt
      661 gtccttcatc agctcggaca attccgaaat tttctatcgt tttaccgacg gcaatctcac
      721 gcgtgagcac cgcggacaaa cctcaacgct gctttctaac cttggcaccg tgcgcttttc
      781 cggcgggcag ttacttccgc agttcgtgtc ggtgttcatc gcgtatgccg acaatcagct
      841 cccatccttt ttcacgtcgg ttgctccgcg aggggtgcgg ccatgatgaa taaccgaggc
      901 atcgcctggt tgctgctcgt cgccttcatt tttcttctgg ccttcattgg cggtgccggg
      961 gtgtactggc gactcaattc gaacttcgtg aaacccccgc tttcgaccaa actgcagatc
     1021 gactaccaac tcgaatccgc actattactg gtttttcaga caattgccgc tgccggccct
     1081 gagcctctcc cggcaacctt cacctacgaa ccgcctcggc gcgagatcat gcccggggtt
     1141 gtcctttctg tgcgcacggt tcaacgaaca ccagaagaag tccaactgga agcccaggcg
     1201 gttggaagcc gacttgaacg gtatctttcc gcccgtctgt tccggggcat tgcctcaggt
     1261 cccgagatgg ccgaacgctc cgcgctgttg ccggcactcg acgcggcatc agcctctgcc
     1321 gatctgagtt cccccagcac cgacctcacg accggctcgc cgacctggct gatcgaactg
     1381 ctgcagcggc gcaccgacac tccatgagca agtcggcccg agcattgccg gtctcgggtc
     1441 acccgcgagc agggccggcc gaatcgctcc tgatgcaggg ttccttcaca catcagcgcc
     1501 tgtcaccgac acgaaccacg cgcgggtttc tgttgatgat cgttctggtc atcggcacga
     1561 ttctgctgct gctcggcatt accctgcatt tcgccatggg gcgtcagaac gcgaatctgc
     1621 actggctggc gaacggcgag atcgcccact ttctggctga cgccggaatc aacagctgca
     1681 tccgtgccgt ccgcgagtcg gttcagactt cggggctgct gaaccagggt tcttcaacca
     1741 acccattggt tcagatactc actcgaccgc aaccgctcga cgacacctcg ttaatgtcct
     1801 atctcaagga cacctggaac gctgaattga aagccttcgc cgccgaagtc gatccgacgg
     1861 ccagcatcag ggtcgaagtc tggctgcgcg acttccgaca gacggaaacc gaccccgccg
     1921 cctgggttga tccagtggca aagcgggggt atctgtctat cgaggcaacc ggtgaatacc
     1981 gaggcgtcaa gcgaaccctg acggtgaaac gtcagatacg agttgccagc tgcctgcccc
     2041 cgctcgtcag caagttcacc ctgcacgtca aaaacgcagg cgatggcggc gaaggcaagt
     2101 tcaacatcat tcgcaacgac tacaacggga acctgaccga cggaccgcgg ccattcattt
     2161 gttacaacca tgcgacaccg gatgctccat tcgaagcccg gccacacgat cctgcgggtg
     2221 aggcggatgc ccaggtgttt gagaaacgtg gctggatctg gctcggcaac caacgcgtcc
     2281 gcctcaacct gacttctggt cccggtctgt tcggcgaaat ctttcacttt tacgaggtga
     2341 aggatccgag tcggtttcaa gccgtgggtt tcgtcactcc cgtcgatcgt ctcccggccc
     2401 cgttcagttc accgcaaacc ttgccccgcg acaacttcca ggccgggcag atggcggaat
     2461 acacattcgg acacagcttt atcttcgatg ggttccatga caaaagcaat cgtcgcgaca
     2521 ccgaagcgat gtacgaaggg agcatcctct cgaatgctga aaaaagcgct ttcggagcga
     2581 aatcctcgtt gctgcatctc tacggcgaag ctcggcccgg cttcatgtcg cgcacgcgag
     2641 tcctgggaaa tgtccaggtt gccctacccc gttttgcaag tctggacatc actccgagtg
     2701 acagtgatct gagaactcga ttttccggtc agaccccgcc accgacctat ctgctgccct
     2761 cggtgccaca gagctccttt gatcccaacc gacagatcaa ggatttcctc gggcgacgtt
     2821 tcggcggacc ggtgatgatc aacgcgctgc tgttcggcac ccagtatgaa gaatacaaga
     2881 agttcatgag cggcatcatc gagctgccct atgtcactgg gtacaactcc atgcaggatt
     2941 atcatgtcga cccgaccaaa cgccagtttc caccgaagca ccagattctg tcagaggacc
     3001 gcggcctgag cctcgaactc aaacgtggtt cagacgtggt ctataaaggc tcagttgatg
     3061 ctcaacgcct cgacgacgtc gtggaaggtc gagtccagaa agtcgtgcca accatcagcg
     3121 aattctggaa gacttatctc gatcgcacca gcggagaact tcatttgaac ggcatcgtgg
     3181 caattgagaa cccggacaga caaaacctga tcctgccgcc gtcagacaag tcctcaccac
     3241 tcaaagtccg tgggggaggt ctcatcctgc tggagcaggg aaatgtcacc ctgcgcggag
     3301 tacaacttct gtcttcgtca gaagctctga caatcattgc tccagaggcg aactctgttc
     3361 gtgtcgtgaa tgcgatttca cagcaggtca atgtcgtggc ccccatggcc gagttcgtct
     3421 gcgacgacaa actcgactgg ttcggcacgc tgtcggtcag cagcattcag gcgaatccac
     3481 gcaatccggg gggcgttctg cggtttcgtg agactcagga tccgacccgc cccgagtctc
     3541 tatcgtttgc caagattcac atcgacgaaa gtgactcgtt ctggcatgag taactcacga
     3601 tgcggcatgt cgctggtcga gattctggtc gccgccggtg tcctttccct gatcctgctg
     3661 ccggtagccc tgtctttcag ttccggtgca cgcggcattc agatgactca cgaggagatg
     3721 acggcccacc aggcggcaat cgaattgatg gaacagctgc agtctctgcc attcgccctt
     3781 gttccagccg gcacgtgggt gaatgatcag attcgtggcg gcatgcggct cgtacaaaat
     3841 tctccgctca cgtttcaggt aacccctctc gacgggtttg aacgcggact gtcaatcacg
     3901 gagctgagca aagacggtcg tgtgagattc aagaagatcg atatctccgt ctcctggaaa
     3961 ggtcaggacg gcaagggacc ggcccgctca tttcgggtga agggattact ggcaaatgag
     4021 accctgtaac ctggcgacct ggtctcgacg agcggggatc actctggtcg agattctgat
     4081 cgtggtggtc attgccgtgt tactcatcgg gacgctgacc accctgttct ccggctttcg
     4141 ccgcagtttc gccaaaggcg aggaatcgac ggtcgtcatt caggaagggg gattcttcct
     4201 tgctcacttg cgcaatgatc tgatcaacgc cgtcttgccc ccggggttcg atcaggctga
     4261 ctggcagaaa gcgatcgtgg ccgacgccga ctcgctgtct ttttccctgt atgctgatgc
     4321 ccagggaacc atcgagaccg tcacctacat gaccgatggc aagtcgctca aacgcatggt
     4381 gaagaacggg tctccgaaac cgttgatcga ggggcgtatt gccagcctct cctggaaaac
     4441 cggctgggaa gtcattccca cgccaggctc cggaactcgt cggctctggg tggaactgtc
     4501 agcaacgctg ggcggccagg ggaagcccgg gatgacctcg aagcagcttc acctgcgcac
     4561 ccggttgttt ccagcccgcc tcaaccgcca gctcaacggc aatctctcgc catgaaggcg
     4621 atcatccgac tcctcctgcc cctgctgctg cccattctca tctcggttct gccattcatc
     4681 atcctgcgcg acgaacgcaa tcgtgcgctg caattggcag gcgagcgtct caagcgcagt
     4741 tggcaccgcc aggcggagtt catcgcgcag cagttccgtt cgaactgctc gatcgaaaag
     4801 cagattcaga atcagaccgg cactctgaaa cacctgctgc agcaagccgc ccggcgacag
     4861 ctggaaaccc ggcatttgct gacctcacag cactttctgc gcagttatca tcaggcgttt
     4921 ccgccccgac gacatccgaa tgggcgcgtc ttctggaatc ctgcctctga atctctgctc
     4981 ctggccttca ttccggatgg cgactcccgg gtcaaagccc ttccgacctc atcactgcct
     5041 tcctatcgtc tgcgaccgtt cacgcggttg atgaacgaga tgctgcaggc gccgcgcctc
     5101 agcaaacagg aactcaccag caaggattcc atcagcaagg ccgtgttcgg tgcctactgt
     5161 caattcgaga tgatcgcact gactcagaag ggcattccca ctccggtgca cttcatggat
     5221 cagcggtcat tcatcgtctg ggataccatc gaagtcaacg ggaaaaggat gggagccttc
     5281 ctcctcatct tctccgagtc gatcgaaacc cgggggcgac cgcttcgtca tgc
//
LOCUS       DAORHD010000134         5312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16033_length_5312_cov_2.925433, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000134 DAORHD010000000
VERSION     DAORHD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16033_length_5312_cov_2.925433"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PKO06_04140"
     CDS             complement(<1..1669)
                     /locus_tag="PKO06_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68864.1"
                     /translation="MSTNTHAASPTIDTVASLRPSWGKRAVSGLFVALPLLMVLAMGH
                     VLLDLHRELFQEEQRRELTGRMSRLADEANPFHFMPKMARELHSRLRAEGMTPEAIDR
                     VCREASALATVSFDLYVFDAAGTLIESRTHPEETRTFMQTVWLYHCTQDNQYYDPNFS
                     RIKQYFGQYFNFQYFQRRQGQALPMNGRNGNGLILWLEGPQRKRDGLLLAVSEVPPLA
                     HFLTRAVKAIEQSGISLWITGADGVERQVLGQEDVRADLAGQVGMRLQLEESLQFQNV
                     RVRAVANPVWKLAPMKSALNVFFLLLVVVLVVGSLYYGEYVDQIYISIKTKLYFLFLY
                     LTLLATLGLATLGLRQSSDREAVAFADAGKAAQEVLAHLDSDFSLARLDCLRFFRSLR
                     DDPHLRAGDLKTFERRLRGQVLNWLEVRHLSGEPFFTTQKRDRDIEFVNRANAKRNIE
                     RFIPERLPAGTTMTYTPPEIMVQSVVETPVSGWLRIFEAPDDIHIMQFGQFALWFYWD
                     AYRQPDSPYATITMNKNQHVAAWEYLTKRMLERFSHGQTALRIVAWRS"
     gene            complement(1669..>4001)
                     /locus_tag="PKO06_04145"
     CDS             complement(1669..>4001)
                     /locus_tag="PKO06_04145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68865.1"
                     /translation="GKAISYLQRGQRGMLFWQRFPEQQGRGVLIVIWKLPDQIAMFER
                     TVRQRLGRHRSLYARQDKGDPTWVAGVPRSTLTRAYLGLAGSERSAWFDADTGWTSVQ
                     AGATHLLYARDFPASAPDQWRHRVLVAWPLILLVVSWLGAILFGPRARVFLSIRWKLV
                     FLFGLALYLPLVGVVFLGVRTLGDRREVLEIGVSRQARETISRLDGRFAGDSLEFTRL
                     CRSLRDHPSMRSDPREMMNVVRPLIDRQKIIHWEVRSLQGDLLCSLGAPGFMEGLESF
                     FESMGKAGILRRLADRFTATGLKMLRPPDPIIEKALLSSSLGFVNIVETPDYVHSSHV
                     GNVLLYWYWDACRESDSPAAMVSIFQSLPYAFRQFLQKQLPDPEIQREGFELVARDPA
                     SGRVFPTGAVPSQEMVELLDRIQVSQEPVRGTVTWNGGHWLVTGAPGKHLQGYALLAL
                     YPAERVEQRVAGVRWAIIGMLALVLVFAALTGLILSRTFLDPIGEIERGLQALQRRET
                     GYRAGWQRADELGHLIQSFNVMIEDLKEMELARVVQETLTPQAFPEIAGYDGRVSMTT
                     ASHLGGDYADVVKLADGRVLIAIGDVTGHGLGAALLMTMVKAGVFQFAEKPTTLVDLR
                     TELNALVFQHFQRRMMMTFFVATLEPRTGEVEYLCSGHPYPFIASKGQVIRQFADPQM
                     PLGASGRRAVVPLRTDRLAPGECLVLYTDGCYEATDPTEKMLGWKRFSEIMAESVRLG
                     IEQGVAGFWRAFRQHRTDEHLEDDCTIILIQHREQH"
     assembly_gap    4002..4011
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4012..4502)
                     /locus_tag="PKO06_04150"
     CDS             complement(<4012..4502)
                     /locus_tag="PKO06_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68866.1"
                     /translation="MSPTITPSSPGYGRLPALGRTLLLVFLLGWVPLLIAGFLCLRVL
                     DGWSELVRERRTDTLARELNRISRLLDPEVSLQSSFPNWAREALTGIGASDATRLERR
                     LGRMVGEQVTIVTFDSNGNIDQKRNDSISNRYVVEKLIRILSRPAEEAIALMRQNRKR
                     LNQV"
     gene            4730..>5312
                     /locus_tag="PKO06_04155"
     CDS             4730..>5312
                     /locus_tag="PKO06_04155"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNV68867.1"
                     /translation="MPYAQNALEPYISSATLGFHHGKHHAAYVKKANELMIAPRFEGQ
                     QPLEVLAAAAKDPAFTGLLNNLAQAVNHEFFWQCLKPNGGGEPTGPLADAVVKSFGSW
                     AKCRAELESAGTAQFGSGWVWLVADGATLKVVKTGNAETPITQGLKPLLTIDVWEHAY
                     YLDYQNRRADFIKVVLDHLVNWDFVTENYLRAGP"
BASE COUNT         1167 a         1675 c         1451 g         1009 t
ORIGIN      
        1 ccgagcgcca ggccacgatc cgcagagccg tctgcccatg cgaaaagcgc tccagcatgc
       61 gttttgtcag gtattcccag gcggcaacat gctgattctt gttcatcgtg atcgtcgcgt
      121 aaggcgaatc cggctggcga tacgcatccc agtagaacca gagggcaaac tgcccgaact
      181 gcatgatgtg aatgtcatcg ggagcttcga agatccgcaa ccagccggaa acaggcgttt
      241 caaccacact ctgaaccatg atctccggtg gcgtataggt catcgtcgtg ccggccggca
      301 gtcgctcggg aatgaaacgt tcgatgtttc tcttcgcatt ggcgcggttc acgaactcga
      361 tgtcgcgatc acgcttctga gtcgtgaaga acggttcgcc gctcagatgg cgaacttcca
      421 gccagttcag cacctgtccg cgcaggcgtc gttcgaacgt cttcaggtca ccggcccgca
      481 gatgcggatc atctcgcagc gagcgaaaga agcgcagaca gtccagacgt gccagagaga
      541 aatccgagtc gagatgggcc agcacttcct gcgcggcttt tccggcatcg gcaaaagcca
      601 cggcttcgcg gtcgctggac tgccgcagac cgagcgttgc caaacccagc gtcgcaagca
      661 gggtcagata cagaaataga aagtacagtt ttgttttgat tgatatatag atctgatcaa
      721 cgtattctcc ataataaaga gagcctacaa ccagaacaac caccagcaac aggaagaaca
      781 cgttcaaagc agacttcatg ggtgcgagct tccataccgg attcgccacg gccctgaccc
      841 ggacattctg aaactgcagc gactcctcca gctgcagacg cattcccacc tgaccggcaa
      901 gatcagcgcg cacgtcttcc tgaccgagca cctggcgttc gactccatcc gcgcctgtga
      961 tccagagtga gattcctgac tgctcaatcg ctttcaccgc gcgagtcaga aagtgagcca
     1021 gtggcggaac ctcactcact gccagtaaca gcccatcgcg ttttctctgc ggcccttcca
     1081 gccacagaat caagccgttg ccgttgcgtc cgttcatggg aagagcctgg ccctggcgac
     1141 gctgaaaata ctgaaagttg aaatactgtc caaagtactg cttgattcga ctgaagttcg
     1201 gatcgtagta ctggttgtcc tgagtgcaat ggtagagcca gacggtttgc atgaacgtac
     1261 gggtctcttc cgggtgcgtc ctgctttcaa tcaacgttcc agccgcatca aacacataga
     1321 gatcaaaact cacggtcgcc aaagccgagg cctcgcgaca gacccggtca atggcctcag
     1381 gagtcatgcc ctcggcccgc agacgggaat gaagctcacg cgccatcttg ggcatgaagt
     1441 gaaacggatt cgcctcatcg gccagacgcg acatccgacc cgtcagctcc cgacgctgct
     1501 cttcctggaa gagctcccgg tgcaaatcaa gtaacacgtg tcccatcgcc agcaccatca
     1561 acagaggcag agcgacgaac aggccactca cggcccgctt tccccaggac ggacgcagag
     1621 aggcgaccgt atcgatcgtc ggtgatgcag cgtgggtatt ggtactcatc aatgctgctc
     1681 ccgatgctgt atcagaatga tggtacagtc gtcttccagg tgttcatcgg ttcggtgctg
     1741 ccgaaacgcg cgccagaacc cggcaacgcc ctgctcaatt cccaaccgca ccgactcagc
     1801 catgatctcg ctgaatcgct tccagccgag catcttttct gtcgggtccg tcgcctcata
     1861 gcacccgtcg gtgtacaaca ccagacactc gcccggggcg agtcgatccg tgcgcagcgg
     1921 aacgaccgcg cgtcgccccg aagccccgag cggcatctgc gggtccgcaa actggcgtat
     1981 cacctgacct ttactggcga tgaatgggta cggatgacca gagcagagat actccacctc
     2041 gcccgtgcgc ggctccagag ttgccacaaa gaaggtcatc atcatacgtc gctggaaatg
     2101 ctgaaacacc agggcgttca gttcggtacg caggtcgacg agcgtggttg gtttctcggc
     2161 gaactgaaag actcccgcct tcaccatggt catcaggagc gcggctccca gaccgtgacc
     2221 ggtgacatcg ccaatcgcga tcagcacccg gccatctgcc agcttcacga catcggcgta
     2281 gtcgccaccc aggtgactcg ccgttgtcat cgaaacccga ccgtcatacc ccgcgatctc
     2341 gggaaacgcc tgcggggtca gggtttcctg cacaactcgc gccagttcca tttccttcag
     2401 gtcttcaatc atgacgttga agctctgaat cagatgtccc agttcatccg cccgttgcca
     2461 tccggcgcgg taaccggttt cgcgcctctg cagcgcttgc agtccacgtt cgatctcacc
     2521 gatgggatca agaaaggtgc gcgagagaat gagcccggtg agcgcggcaa aaaccagaac
     2581 cagggccagc atgccaatga tcgcccagcg cacgcccgcg actctctgtt ctacccgctc
     2641 agccggatat aaggccaaca gggcatatcc ttgcagatgt ttccccggag cgccggtgac
     2701 cagccagtgt cccccgttcc aggtcacggt cccgcgaacc ggttcctgac tgacttgaat
     2761 gcgatccagg agttccacca tctcctggct gggcaccgca cccgtcggaa agacccgacc
     2821 actcgcgggg tctcgcgcaa cgagctcaaa tccctcccgc tgaatctccg ggtctggtag
     2881 ctgcttctgc agaaactggc gaaacgcata gggcagcgac tgaaagatac tgaccatggc
     2941 ggccggggaa tcggactcgc gacaggcatc ccagtaccag tacagcagga cgttgccaac
     3001 gtgcgacgaa tgcacgtagt cgggcgtttc cacgatgttg acgaatccga gtgacgacga
     3061 caaaagtgct ttctcgatga tcggatcggg cggccgcagc atcttcaacc ctgtggcggt
     3121 gaatcgatcc gccagccggc gcagaattcc cgcctttccc atgctctcga agaacgattc
     3181 gagcccttcc atgaatccag gagctcccag cgagcacagg agatctccct gcagcgaacg
     3241 aacttcccag tgaatgatct tctgacgatc gatcaggggc cgcacaacgt tcatcatctc
     3301 ccgcggatcg gagcgcatcg atgggtgatc gcgcagtgat cgacagagac gggtaaactc
     3361 gagactgtcg ccggcaaacc tgccgtccag gcgactgatc gtttcgcggg cttgtcgcga
     3421 aaccccgatt tcaagcactt cgcggcgatc gccaagggtt cgaacgccga gaaaaaccac
     3481 accgaccagg ggaaggtaga gagccagccc gaagagaaag acgagtttcc agcgaatcga
     3541 caggaacact ctggcacgtg gcccgaacag gatcgccccc agccacgaaa ccacgagcag
     3601 gatcagcggc caggcgacca gaactcggtg ccgccactgg tccggagctg atgcgggaaa
     3661 atcccgcgcg tagaggagat gggtcgcgcc cgcctgcact gaagtccagc cggtatctgc
     3721 atcgaaccag gctgagcgtt ccgagccggc caatccgaga tacgcgcggg tcagcgtcga
     3781 gcggggcaca cccgcgaccc acgtgggatc tcctttgtcc tgccgcgcat acagcgaccg
     3841 atgccggccc agtctctgac gaaccgtgcg ttcgaacatc gcgatctggt caggaagttt
     3901 ccagatgaca atcagcacgc cgcggccctg ttgttccggg aagcgttgcc agaacagcat
     3961 gccacgctgc ccgcgctgca gatatgaaat cgccttgcct tnnnnnnnnn nacttgattc
     4021 aaccgtttcc ggttctgacg catcagcgca atcgcctctt ccgccggacg cgaaagaata
     4081 cgaatcagtt tctcgacgac gtaccggttg ctgatcgaat catttctctt ctgatcgata
     4141 tttccgttgc tgtcaaaggt gacaatggtc acctgttctc ctaccatgcg tcccagtcgt
     4201 ctttcgagtc gagtcgcatc tgaagcgcct atccctgtca gggcttctcg tgcccagttc
     4261 gggaaactgc tctgtagact gacctccgga tccagcaggc gcgaaatgcg gttcaactcg
     4321 cgggccagag tatcggttcg ccgttctcgc accagttcgc tccacccatc gagaacccgc
     4381 agacagagaa atcctgcgat caacaggggc acccatccca gcaggaacac gagcagcagc
     4441 gtacgcccca gagctgggag acgcccgtac ccgggagaag acggtgtgat cgtcggcgac
     4501 atattctggg tattttagtg taccgccccc ggaaaagata ccgttttcct gtgaatggat
     4561 tccggccgcg gtgagggtgt atcctgtccg tatcaccttt tccggaggat ctctcatgcc
     4621 cagaaacgcc cgccttttcg cctcgctggt gctactgacc agcctgctgc tgacttcagt
     4681 ccagaccacc cgtgctgatg ccccctgccc tgaagtcgta ttcgctcccc tgccctacgc
     4741 gcaaaatgct ctcgagccgt acatttcctc agcaaccctc ggctttcacc acggcaaaca
     4801 ccacgcagcc tacgtcaaaa aggccaacga actgatgatc gcgccacgct tcgagggtca
     4861 gcaaccgctc gaagtcctgg ccgccgccgc gaaagatcct gcgttcaccg ggttgctgaa
     4921 caatctggca caagccgtca atcacgagtt tttctggcag tgcctgaaac cgaacggcgg
     4981 tggtgaaccg accggtcctc tggcagacgc cgtggtaaag agcttcggca gctgggccaa
     5041 atgccgcgcg gaactcgagt cggcaggaac ggcacagttc ggcagtggat gggtctggct
     5101 tgtcgccgat ggggccacgc tgaaggtggt gaaaacgggc aatgcagaga cgccgatcac
     5161 ccaggggctc aaaccattac tcacgatcga tgtctgggaa cacgcctact acctcgacta
     5221 ccagaatcgc cgcgccgact tcatcaaggt cgtgctcgac catctcgtga actgggactt
     5281 cgtcaccgag aattatctcc gggccgggcc ct
//
LOCUS       DAORHD010000135         5309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16045_length_5309_cov_3.381424, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000135 DAORHD010000000
VERSION     DAORHD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16045_length_5309_cov_3.381424"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKO06_04160"
     CDS             <1..334
                     /locus_tag="PKO06_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68868.1"
                     /translation="PATPTTVKKVSPQPTAIQDQPAAFPAPASPRPPSPPESQAPRIG
                     ETLIIPEGQSIIVRRGPSERLARIARLPGGTRMQVKFVRHVGKTIWLEVNFGKGKSGW
                     IPARDFPL"
     gene            426..932
                     /locus_tag="PKO06_04165"
     CDS             426..932
                     /locus_tag="PKO06_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV68869.1"
                     /translation="MNLNRRGFNLVEMLIVVAVLSFLTVLALPLTEITYHRAGERRLR
                     ERLGEIRQAIDAYRRSHRLGQLPASIASLTAPIAASDLRVGANEGPFLAYGSLGNPFT
                     SPPDTFHWELRLFNPSAPSHNTWQIVINNASTTFGTGTGVFDIRFPLAGVNGLATSPL
                     DGTVYGTW"
     gene            919..1194
                     /locus_tag="PKO06_04170"
     CDS             919..1194
                     /locus_tag="PKO06_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68870.1"
                     /translation="MAPGKPTRGFTLLELILTILILSALVALAVPYYQSYLEDSKRAV
                     MIANLQTIRKTLIEYKSDTGTYPISLTDLMPKYFFELPEDPINATNT"
     gene            1255..3708
                     /locus_tag="PKO06_04175"
     CDS             1255..3708
                     /locus_tag="PKO06_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV68871.1"
                     /translation="MDTNLLSTLIDLVPSPVYLKDARGAYTFCNRAFCEFLQLPRERI
                     VGHTFLELGVATADKAGEYLRHDQQLIQSQNEATELEDTITFTDGRTRHVIIHKSRVI
                     AAEGSTRGILGVIHDVTSLVESKQQSEALTQELKQALANAETLRRQADQARAEAETLM
                     LTAESANRAKSDFLANMSHEIRTPMNGIIGMVGLLLNTPQSPEQCEFSRTIKMCAESL
                     LGLINDILDFSKIESGKLEIENIDFDLRTTIEDVIDTLAFKAREKGLDLFYLIEPAVP
                     ALLQGDPTRLRQVMVNLVSNAVKFTNEGEVAIHVTLDHEDQDQVIIRFSITDTGVGIP
                     PAQLPRLFKPFTQADSSTSRRFGGTGLGLSICKRLVDMMDGQIGVRSREKAGSTFWFT
                     ARFPKQKGEALPTFDPSSVEITGSRVIIVDEHRTDCRVFENMLASWKCRCEAVPTGLQ
                     ALRLLREANVRKDPFKFAIIDWQLRDLRGDELGRILRNDPDFSSLILIAISPGNREVS
                     GKVLESAGFEATLQKPFKQSQLYNSLLMTVAGRAGNISVSGPMITSQAADNLPKRQLK
                     ILLVEDNLVNQRVALKILENLGYVADAVLNGVEALRALAKVNYHLVLMDVQMPEMDGF
                     ETTRHVRNGPERVHNPRVPIIAMTANAMRGDREECLSAGMDDYIAKPVEPDELAQVVG
                     KWLNPVLEEGRQPSEKRLAPDAMVFDRAGFLTRLGGNEQILEQIIDCFLLQDVPQQIR
                     SLKEAIEAGEIAKIQRLAHTLKGASSSVGALALTDAALKLEVAVKEGRSEQFAGLLGG
                     IEHAFAELKRLLGGSSGAA"
     gene            complement(3710..>5309)
                     /locus_tag="PKO06_04180"
     CDS             complement(3710..>5309)
                     /locus_tag="PKO06_04180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV68872.1"
                     /translation="PVDQVVNLGIMTGFPDGRFQPHKPMTRGEATVIFSRLIQALEKA
                     FFRKPPILPIRPKYADVPDGHWLNGDLARIGGVGGLRCFPGERFRANDSLRLIELRQL
                     RTALLDYFGENLFILSGTPPELLLLPKGVFQRLTCQDWSYSMDQVEWTDVPPNGRIKL
                     PEGGSPTRLWLKNPDFQTIGPVEVRSDQLNFWLLLVRKDLRKFRDRLLAERNKEFETD
                     GSDRLGFHLQSLRARLKKPDETPAPPKRDDAETIVTLMEGMHAEEGSGEETVATALPP
                     TAQTADNPIISDEQRTIQPKWGGGPEIELADGTRGTAKDSAPPRVLAGNAVETRTRHF
                     SREIPDPMSFTGNVIDSLTGKGVAGAVVLADKQTGETDERGVFRFKVSPGAMLDLTIY
                     REGYEPLSLRQRVDRQSPESRFQLKPLFADFGGFVTSEDTGEPIAGAVIRLGEQRARS
                     GPDGRYRFTRVRPTFYQLSGQAEGFMEAVEFVHVSPTREQPHELKLKPVEQAWQRREA
                     PEAQGDGSINEARIDAVSSLPPIE"
BASE COUNT         1122 a         1646 c         1441 g         1100 t
ORIGIN      
        1 gccagcgacg ccgacgaccg tcaagaaggt cagtccgcaa ccaacagcga tacaagatca
       61 gcccgccgcc tttcccgctc ccgcatcacc ccggccccca tctccgccgg agtcgcaggc
      121 tccccgcatt ggcgaaacgc tgatcattcc ggaaggacaa agcatcatcg ttcgacgtgg
      181 acccagcgaa cggcttgccc gcatcgcccg cctgcccggg ggaacccgaa tgcaggtgaa
      241 gttcgttcgt catgtcggca agacaatctg gctcgaggtc aacttcggga aagggaaatc
      301 cggctggatt ccggctcgag acttcccctt gtaacgttga cgccgctgag ccgacgcacc
      361 ccgagaaata cctgtcgcca gggacgtccc gtcacggtag aatggtgtcg tctacggaaa
      421 acgtcatgaa tctgaatcgt cgtggcttca atcttgtcga aatgttgatc gtcgtcgccg
      481 tgctcagttt tctcaccgtg ctggccttac cgctgacgga aatcacgtat catcgcgccg
      541 gcgaacgtcg tttgcgcgag cggctcgggg agattcggca ggccatcgat gcgtatcgac
      601 gctcacaccg gcttggtcag ctccccgcgt caatcgccag tctcacggct cccatcgctg
      661 cgagtgatct gcgtgtcggg gccaatgagg ggccgtttct ggcctatggc tcactcggca
      721 acccgtttac atcacccccc gatacctttc actgggaact acgcctgttc aacccctcgg
      781 ctccatcaca taacacctgg cagatcgtga tcaacaatgc ctctactaca tttggaactg
      841 ggacaggcgt cttcgacatt cgctttccgc tggcgggagt gaatggtctg gccacttctc
      901 cccttgatgg aaccgtctat ggcacctggt aaacccaccc gcggattcac tctgctcgag
      961 ctcattctga ctattctgat tctgtctgct ctcgtcgctc tggccgtccc atactaccag
     1021 tcgtatctcg aagacagcaa acgcgcggtc atgatcgcga atctgcagac gatacggaaa
     1081 acgctcatcg agtacaaatc tgatacggga acctacccca tttctctcac agatctgatg
     1141 ccgaaatact ttttcgaact cccggaagac ccgatcaatg ccacgaatac gtagggctat
     1201 ccgaatctct cgacccgtga tagacttggc gtcatctacc aggatctcta gcagatggat
     1261 acgaaccttc tttccaccct gattgacctt gtcccgtcac cggtctacct gaaggacgcc
     1321 cgtggggcgt ataccttctg caatcgcgcc ttctgcgagt ttctccaact tccgcgtgaa
     1381 cgcatcgtgg gtcacacgtt cctcgaactc ggggtcgcca cggcagacaa ggccggggaa
     1441 tacctgcgcc acgatcagca gctgattcag agccagaatg aagccacgga gctcgaagac
     1501 accatcacgt tcaccgatgg aagaacgcgt catgtcatca tccataaaag tcgcgtgatc
     1561 gccgccgaag gatccacgcg aggaattctc ggcgtcattc acgatgtcac gtctctggtc
     1621 gaatcgaagc agcagtctga agcgttgacc caggaactga aacaggcgct ggccaatgcg
     1681 gagacgttgc gacgacaagc tgatcaggcg cgcgccgaag cggaaaccct gatgctcacg
     1741 gccgaatcag ccaatcgtgc gaaaagtgat tttctcgcga acatgagcca tgagattcgc
     1801 accccgatga acggcatcat cggcatggtc ggactgctgc tcaacacgcc gcagtcgcct
     1861 gaacagtgcg agttttcgcg caccatcaaa atgtgcgctg agtcgcttct ggggctgatc
     1921 aacgacattc tcgacttttc caagatcgag tccggcaagc tcgaaatcga gaacatcgac
     1981 tttgatctgc gcaccaccat cgaagacgtc atcgacactc tggccttcaa ggctcgggaa
     2041 aaagggctcg atctgttcta tctgatcgaa ccggctgttc ccgccctgct gcagggtgac
     2101 ccgacccgcc tgcgacaggt catggtcaac ctcgtcagca atgcggtgaa gttcaccaac
     2161 gaaggcgaag tggcgattca cgtcaccctc gaccacgaag accaggatca ggtcatcatt
     2221 cgcttttcca ttaccgacac cggagtcggc attcccccgg cccaactgcc acgattgttc
     2281 aagccattca cacaggctga ttccagtact tcacgccgtt ttggcggaac tggactagga
     2341 ctttcgatct gcaaacgtct ggtcgacatg atggacggac agatcggagt tcgcagtcga
     2401 gagaaagccg gctcgacctt ctggttcacg gcgcgttttc ccaagcagaa aggcgaagcg
     2461 ctgcccacgt tcgatccgag ttcggtcgaa atcacgggga gtcgcgtcat cattgtcgat
     2521 gaacaccgca ccgactgtcg cgtgtttgag aatatgctcg cctcgtggaa gtgccgctgt
     2581 gaagcagtgc caacgggcct gcaggctctg cgtcttctgc gcgaagccaa cgtgcgcaag
     2641 gatccgttca agtttgccat catcgactgg cagttgcgcg atctgcgcgg cgatgaactc
     2701 ggacgcattc tgcgcaacga cccggacttc agcagcctga ttctgatcgc gatttctccg
     2761 ggtaaccgcg aggtgagtgg caaagtgctg gaaagcgccg gtttcgaagc cactctgcag
     2821 aagccgttca aacagtcgca gttgtataac tcgttgctca tgaccgtcgc ggggcgtgcc
     2881 ggcaacatct ccgtttcggg accaatgatc acctcgcagg ccgcagacaa cctgccaaag
     2941 cggcagctga agattcttct cgtcgaagac aacctggtca atcagcgagt ggcgctgaag
     3001 attcttgaaa acctcgggta tgtcgctgat gccgttctca acggggtcga agcgctgcgc
     3061 gccctggcca aggtcaacta ccatctggtg ttgatggatg ttcagatgcc cgaaatggat
     3121 ggctttgaaa ccacccgcca tgtgcgcaat ggtccagagc gcgttcacaa tcctcgggtg
     3181 cccatcattg ccatgacggc gaacgcgatg cgcggtgatc gcgaagaatg cctgtcagcg
     3241 ggaatggatg actacatcgc caaaccggtt gagcccgacg aactggccca ggtggtcggc
     3301 aaatggctga acccggttct cgaggaaggc cgacaaccgt ccgaaaaacg actcgctcct
     3361 gacgccatgg tcttcgaccg cgccggcttt ctcacccgtc tgggcggcaa cgagcagatt
     3421 ctcgaacaga tcatcgactg tttcctgctg caggatgtcc cgcagcagat tcgcagcctg
     3481 aaagaggcca tcgaagccgg agaaatcgcc aagatccagc gtctggctca cacgctgaaa
     3541 ggagcgtcga gttcggtcgg cgctctcgcg ctcaccgacg cagcactcaa actggaagtg
     3601 gcggtgaagg aagggcgttc agagcagttc gctggtctgc tgggcgggat cgaacatgcc
     3661 ttcgccgaac tgaaacgcct cttgggcggc tcttcgggag ccgcctgatt cactcgatcg
     3721 gcggcaggct cgagaccgca tcaatccgtg cctcattgat actcccgtca ccctgcgcct
     3781 caggagcttc gcgtctttgc catgcctgtt cgactggctt cagcttcagc tcgtgtggct
     3841 gttctcgagt cggagaaaca tggacaaact cgaccgcttc catgaatccc tccgcctgtc
     3901 cggataactg ataaaaggtc ggacgcaccc gcgtgaaccg ataccgtcca tccggcccgc
     3961 tcctggctcg ttgctcgcca agccgaatca cggctccggc aatcggctct cccgtgtctt
     4021 cactggtcac gaagccgcca aagtcggcaa agagcggctt gagttgaaaa cgcgactcag
     4081 gtgactgtcg atcgacgcgt tgtctcaggc tcagcggctc gtacccttcg cggtaaatcg
     4141 tcagatcgag catcgctccc ggcgagacct tgaagcgaaa cacgcctcgc tcatcggtct
     4201 ctcctgtctg cttgtcagcc agaaccaccg cccccgcaac acccttgccg gtcaaactgt
     4261 caatcacgtt accggtgaat gacatcggat cagggatttc tcgtgagaaa tgccgggttc
     4321 gcgtctcgac agcattcccc gcaagcaccc ggggcggcgc gctgtctttc gccgttcccc
     4381 gtgttccgtc agcgagttcg atctccggtc cgcctcccca tttcggctgt atggtgcgct
     4441 gctcgtcaga gatgatcggg ttatcggctg tctgagcggt tggcggcaac gcggtggcaa
     4501 cggtttcttc gcccgagcct tcttcggcat gcattccctc catcagggtc acgatcgtct
     4561 cggcatcatc gcgtttcggc ggcgccggcg tttcgtcagg cttcttcagg cgtgcacgca
     4621 gcgactgcag atgaaagccc aggcgatcgc tgccatccgt ttcgaattcc ttgttccgtt
     4681 cggccagcag gcgatcgcgg aattttcgca gatccttgcg taccaggagc aaccaaaagt
     4741 tcagctgatc agaccgcact tccaccggac caatggtttg aaagtcagga ttcttcagcc
     4801 acaatcgagt cggcgaccct ccctccggaa gcttgattcg accattcggt ggcacatccg
     4861 tccattccac ctgatccatt gagtagctcc agtcctggca ggtgagccgc tgaaagacgc
     4921 ctttcggcag cagcagcaac tctggcgggg tgccgctcaa gatgaacagg ttctcaccaa
     4981 aatagtcgag caaggccgtg cgcaattgtc gcagctcgat cagtcgtaat gaatcgttcg
     5041 cccgaaaacg ctctcccgga aagcagcgca gaccgccaac gcccccaatg cgggccagat
     5101 cgccattcag ccaatgccca tccgggacat ccgcgtattt cggacgaatg ggaagaatcg
     5161 gcggtttgcg aaagaacgct ttttcgagcg cttgaatcag tcgtgaaaag atgaccgtgg
     5221 cctcgccacg ggtcatcggc ttgtgcggtt gaaagcgccc atcggggaat ccggtcatga
     5281 tcccaaggtt cacgacctga tcaaccggc
//
LOCUS       DAORHD010000136         5307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16056_length_5307_cov_2.995811, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000136 DAORHD010000000
VERSION     DAORHD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16056_length_5307_cov_2.995811"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="PKO06_04185"
     CDS             <1..859
                     /locus_tag="PKO06_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68873.1"
                     /translation="QAEAYFSELPFELAPATDDSPFCFNCERWSELLPALLRLNLGAV
                     SYGEWPSLILLSLLLLSGLLTLLFIFVPLYLAAATDFSHAQRPVILYFCLLGVSFIFI
                     EISLMQRFSLLLGHPSYSLTTTLGSLLLFSGIGSMMAGRFAILGRLSFGAIVMLGLCA
                     AWGYPDLMRFLLPYSLPIRVFGVILLVAPLGFFMGMPFPLGLRDLGTRAPHLVPWAWG
                     VNGGATVLGSVLAIVTAMKCGFTMVLLLASCGYLLAWVSFRMSSVSGPPAVVTPSITA
                     VHPEPPSGG"
     gene            864..1937
                     /locus_tag="PKO06_04190"
     CDS             864..1937
                     /locus_tag="PKO06_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68874.1"
                     /translation="MSLSPYPAFAPPWDASSWVVFDRCALLANLAARLGPANSLLDLR
                     SPILSMLAPHFEDDWRRLGVGGFLISASHLSRLPSSFYRNWQGILTAPFRSSALSLVA
                     RLGLCPIISQVPEAIIWSNMAQQAGKELPLLVRWRGGDLLADSGPTGFLPLVEAIPGL
                     PMLRLDGFFCDTPFASRHQADAFRRAISRTCPESTTPILISGSAGELPARIKPLSTID
                     DEFLTWTETVEQAATFTVETWGVPVDQTSNQLHLSIDFGSSHGIPDHMELPVLVEDMP
                     GVLYRVEPYRSLIALPHRPEARTPWRVTLVGGNGVQAIGPSLWPRALLRRFIADRLRT
                     VPCYLASANGYELITSAQGAEAT"
     gene            complement(1930..2955)
                     /locus_tag="PKO06_04195"
     CDS             complement(1930..2955)
                     /locus_tag="PKO06_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV68875.1"
                     /translation="MTSVTLPRKVTEKLQEIPLRGEISYSFASSSSGEGMIGESWLLL
                     TQTQIIVVVQPLVEDLKVAGVFKIQELSQIAVRRTFLGDIRFSFVNAAEAVLAEFEVP
                     SLQKDEGEELFQMLEKRFPGKMRQTREDVLDAVDVTGDSMMTVRVDLMKSIADTTSGV
                     QVDNVSIDSRMSLPGLDSVEGPLGDGVEQTVIIDVPVSQTVEVNTDSSVANPLSHTRE
                     HEQQKSHHAPSVNLKGPKKPKKSGQVVPPPPAPGPAEDDDASVDCTKCGRTNRVSFQY
                     CLACGHQLPVPPDKHRRRRPVNAVVGASTGENAPDDSGCGSCLGQLIGFIIFLFIFQG
                     ILSAVFK"
     gene            complement(2952..3626)
                     /locus_tag="PKO06_04200"
     CDS             complement(2952..3626)
                     /locus_tag="PKO06_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68876.1"
                     /translation="MADTAKGKGGSGCLPSCGCFCGCWAFVLAVLAVSGGLFITFLAW
                     PFWKALKTTPQFPEMPRIEASDRDSFEEKRVQWQQAQGSGTVEFTLGEVNAWLARFRP
                     PPASGLLVEHVWVVAQDGRLSVLADGSGFFCKRLSMACEFDTFSSSSGDWPLRSWQLN
                     AWVEKSATGLRLGRWFLDHYLQTVTGKDLIGLLAGITKIEFATDRIRIEGVIPEAWKK
                     RQGEEK"
     gene            complement(3628..4113)
                     /locus_tag="PKO06_04205"
     CDS             complement(3628..4113)
                     /locus_tag="PKO06_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004095098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV68877.1"
                     /translation="MAVQKKVQSRQEIQVVGFYLGTDEYAININNVREIVAMMEIRKI
                     PKAPPFVEGVIVLRGHVIPIIDLRKRFEMSAIADIQSAKILIVEFHSHQLGMIVDNVS
                     EVFRLNQNQIEPAPPLFHGNVSSQYIQGVGKLEERIIVLLDVEKLLSGEERTQLASLK
                     G"
     gene            4192..4647
                     /locus_tag="PKO06_04210"
     CDS             4192..4647
                     /locus_tag="PKO06_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68878.1"
                     /translation="MTTNKFRLWKLASDPERPWERRCEDPRGVLFQLVSGESFRHLGI
                     VHCPVVSDGYRGRHAHRRKLEEFFVLSGTLAFFWRDLETGTHGRLELAPGDRLSLRPG
                     LAHAFKALTEAVFVEFCPTPYDPTDVFPFDPLQPPEGWVPDPDFLRKPS"
     gene            complement(4756..5157)
                     /locus_tag="PKO06_04215"
     CDS             complement(4756..5157)
                     /locus_tag="PKO06_04215"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNV68879.1"
                     /translation="MTKEAKLIEAAIEASKRAYAPYSKFFVGAALETTEGEIISGCNV
                     ENASYGLTNCAERTALFSAFAQGKRTFKRMALFVDHHEFIFPCGACRQVLIELAPKIE
                     ILVVKGDREVKKVKPTDLMPYSFTADQLDDR"
BASE COUNT          995 a         1651 c         1424 g         1237 t
ORIGIN      
        1 ccaggccgaa gcgtatttct ccgaacttcc cttcgaactg gcaccggcga ccgacgacag
       61 tcccttctgc ttcaactgcg aacgatggtc agaactgctg ccggccttac tgcgcctgaa
      121 tctcggagca gtctcttacg gagaatggcc atcgctgatt ctgctgtctc ttctgcttct
      181 gtcgggtctg ctgaccctgc tgttcatctt tgtccctttg tatctggcag cggcaacgga
      241 tttcagccat gcacagagac cggtcattct ctatttctgc cttctgggcg tgtcattcat
      301 cttcatcgag atttccttga tgcagaggtt ttcgctgctg cttgggcatc cgtcctactc
      361 attgaccacc actcttggct ctctgctgct gttttcaggt atcggaagca tgatggccgg
      421 ccgcttcgcg attctcggcc gtctgagttt cggggcaatc gtcatgcttg ggctgtgcgc
      481 tgcctgggga tatcctgatc tcatgcggtt tctgctcccg tactccctgc cgatacgcgt
      541 gttcggtgtc atcctgctcg tcgcccctct cggattcttc atgggcatgc cctttccgct
      601 tggtctgagg gatctgggga cacgggcgcc acatctggtg ccctgggcct ggggcgtgaa
      661 cgggggagcc acggtgctgg gttcagttct cgccattgtc acggcgatga agtgcggttt
      721 cacgatggtg ctgctgctcg cctcgtgtgg gtatctgctg gcctgggtca gttttcgtat
      781 gtcttcggtt tccggcccac ccgccgtggt cacaccctca atcaccgcgg ttcatccaga
      841 gccgccctca ggaggctgac tccatgtccc tttcgcccta tcccgcgttt gctcccccct
      901 gggatgcttc ttcctgggtg gttttcgatc gatgcgccct gctggccaat cttgcggcac
      961 gcctgggccc ggcgaactct cttctcgatc tgcgatcacc gattctctcc atgctcgccc
     1021 ctcacttcga agatgactgg cggcgactcg gcgtcggagg ttttctgatc tccgcttctc
     1081 atttgtccag gcttccatca tctttttatc gcaattggca aggaattctc acggctccat
     1141 ttcgttcttc tgcgttgtcg cttgttgccc gactcggttt gtgtcccatc atatctcagg
     1201 ttcccgaagc gatcatctgg tcgaacatgg ctcagcaggc cgggaaagag ttgccattgc
     1261 tggtacgctg gcgtggcggc gatctcctgg ccgactctgg cccgaccggt ttcctccccc
     1321 tcgtcgaggc cattccaggc ctgcccatgc ttcgtctcga cggctttttc tgcgataccc
     1381 cgtttgccag tcgacatcag gccgatgcct ttcgtcgtgc gatctcgcgc acctgccctg
     1441 agtcgacgac tcccatcctg atttcggggt cggcaggaga actgccggct cgaatcaaac
     1501 cgttgtcgac catcgatgac gagtttctga cctggaccga gaccgtagag caggcggcca
     1561 cctttaccgt cgaaacctgg ggagtaccgg tcgatcaaac ctcaaatcag cttcatctct
     1621 ccatcgactt tggatcatct catggtatcc cagaccacat ggaactcccg gtcctggtcg
     1681 aagacatgcc cggtgtgctc taccgcgtcg agccgtatcg cagcctcatc gctctgcctc
     1741 accgtcccga agcacgaaca ccgtggcgcg tcactctggt tgggggaaac ggtgttcagg
     1801 ccatcggtcc atctctctgg cccagagcgc tgctgcgccg tttcattgct gatcgactgc
     1861 gaacagttcc ctgctatctg gcctcagcga acgggtatga gctgatcacg agcgctcagg
     1921 gggctgaggc tacttgaaga cggctgagag aattccctga aaaatgaaga gaaagatgat
     1981 gaacccgatc agctgaccca gacaggaacc gcacccggag tcatctgggg cattctcccc
     2041 ggtcgaagcc ccgaccaccg cattcaccgg gcggcgacga cgatgcttat cgggcggaac
     2101 cggcagttga tgcccgcagg ccagacagta ttgaaaactg acgcgattgg ttcgaccaca
     2161 cttggtacaa tcaaccgagg catcatcgtc ttcggcaggg ccgggcgctg gcggaggcgg
     2221 cacgacctgg cctgacttct tcggcttttt tggccccttc agattcaccg acggtgcgtg
     2281 atgcgacttc tgctgctcgt gttcacgcgt gtgtgacagc gggttggcaa ctgaactgtc
     2341 ggtgttgacc tcgacggtct gactcacggg gacatcgatg atcacggttt gttcgacccc
     2401 gtccccaagc ggcccctcga ccgagtcgag ccccggaagg ctcatgcgac tgtcaatcga
     2461 cacattgtca acctggactc ccgaggtcgt gtcagcgatg ctcttcatca aatcgacccg
     2521 caccgtcatc atactgtcgc cggtcacatc gacggcgtcg agcacgtctt cgcgcgtctg
     2581 acgcatcttg cccgggaaac gcttctcgag catctgaaac agttcttcgc cttcatcctt
     2641 ctgcagtgat ggcacctcaa actcggccag caccgcttct gccgcattga caaagctgaa
     2701 gcgaatatcg cccagaaagg ttcgccgcac cgcaatctgt gaaagttcct gaatcttgaa
     2761 gaccccggcc accttcaggt cttcgaccag cggttgcacc acaacgatga tctgcgtctg
     2821 cgtcagtagc agccaggact caccaatcat gccttcgccc gacgacgaac tggcaaacga
     2881 gtacgagatt tcgccgcgaa gcggaatctc ctggagtttt tcggtcacct tgcgaggcag
     2941 agtcacactg gtcatttttc ttccccctga cgcttcttcc acgcttcggg aatgacgcct
     3001 tcgatcctga tacgatcagt cgcgaactca atcttcgtaa tgccggccag aagcccgatc
     3061 aggtccttgc ccgtcaccgt ctgcagatag tggtcaagga accatcggcc gagacgcagc
     3121 ccggtcgctg acttctcgac ccaggcgttc agttgccagc tgcgcagagg ccaatcacca
     3181 ctgctgctgg aaaaggtatc aaattcgcag gccattgaca aacgtttgca gaaaaagcct
     3241 gaaccatcgg ccagaacact cagacgacca tcttgagcca caacccacac atgttcgacc
     3301 aacagtccac tcgcgggcgg cgggcggaag cgcgccagcc acgcattgac ttcgcccagg
     3361 gtgaattcga ccgtgccact gccttgagcc tgttgccact gtacccgttt ctcttcgaaa
     3421 gaatcacgat ctgaagcctc gatacgtggc atttcaggga actgaggagt cgtcttgagt
     3481 gctttccaga aaggccaggc cagaaaggtg atgaacagtc caccgctgac cgccagcacg
     3541 gcgagcacaa aggcccagca gccgcagaaa catccgcacg atggcaggca cccgctcccg
     3601 cccttccctt ttgcggtatc ggccatttca acctttcaga ctggccaact gcgtgcgttc
     3661 ttcgcccgag agcagtttct cgacatcgag cagcacgata atgcgctctt cgagcttgcc
     3721 gacgccctga atatactgcg aactgacatt gccgtgaaac agtggcgggg ccggttcgat
     3781 ctggttctga ttcaaacgaa acacctcgga cacgttgtcg acgatcatgc cgagctgatg
     3841 gctgtgaaac tcgacgatca ggatcttcgc gctctgaatg tctgcaatcg ccgacatctc
     3901 gaaacgtttg cgcagatcaa tgatcggaat gacgtgacca cgcaggacga tgacgccttc
     3961 gacgaacggt ggagctttcg gaatcttgcg aatctccatc atcgcgacaa tttcgcgcac
     4021 attgttgata ttgatcgcat attcgtcggt tccgagatag aacccgacga cctgaatctc
     4081 ttgccgtgac tgaaccttct tctggactgc catgaccgta tggtagcatg cgttcagaga
     4141 cgatgcaacg aatgatgcga agcgagtgtt cgtgctatac ttccggtaca gatgaccacc
     4201 aacaaatttc gcctctggaa acttgcttca gacccggaac gcccatggga acgccgctgc
     4261 gaggatccgc gcggggtgct ctttcaactc gtcagcggcg agtcattccg gcatctgggc
     4321 atcgtccact gtccggtcgt ctccgacggc tatcgcggtc gccatgcaca tcggcgcaaa
     4381 ctcgaagagt ttttcgtgct ctccggcacc ctggccttct tctggcgtga tcttgaaacc
     4441 ggcacgcatg gccggctcga actcgctccc ggagaccggc tttccttgcg ccccgggctg
     4501 gctcacgcgt tcaaagccct gaccgaagct gtctttgtcg agttctgccc gaccccctac
     4561 gacccgacag atgttttccc ctttgatccg cttcagccgc ccgaaggctg ggttcccgac
     4621 ccggactttc tccggaaacc gtcctgaaag gcgcaacccc cgcgacgggc tttccgtcag
     4681 cgggggttgt tctccgtgat gtgccgtggg gcaggcagaa agatcacccc gacgaatcac
     4741 cgctcagaga ggtgtttacc gatcatcgag ttggtcggcg gtaaaactgt acggcatcag
     4801 atcggtgggt ttgaccttct tgacttcccg atcacccttc acgaccagaa tttcgatctt
     4861 cggggctaat tcgatcaaca cctgacgaca cgcaccacag ggaaagatga actcgtgatg
     4921 gtcaacgaac agcgccatgc gcttgaatgt gcgcttgccc tgtgcgaagg ctgaaaacag
     4981 agccgttcgt tcggcgcaat tggtcagccc gtagctcgcg ttttccacgt tgcagccgct
     5041 gatgatctcc ccttccgtgg tctcgagcgc agctccgacg aagaacttcg aatagggcgc
     5101 ataggcccgt ttgctggctt cgatggccgc ttcgatcagt ttcgcttcct tggtcattcc
     5161 gtcttccttt gtcctgattg cgagagatga aaaaggggcc gatgaacggc cccatttccg
     5221 aaatccgaaa tacgtttagc gcttggagaa ctgtgggcgg cggcgagcgc ggcgcagacc
     5281 gtactttttg cgttcgcgca tgcgcgg
//
LOCUS       DAORHD010000137         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16064_length_5305_cov_4.066286, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000137 DAORHD010000000
VERSION     DAORHD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16064_length_5305_cov_4.066286"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="PKO06_04220"
     CDS             complement(<1..1037)
                     /locus_tag="PKO06_04220"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV68880.1"
                     /translation="MTSEASFRGQGQYRYSWECFISLLFGGVFAVCFYFGLFTNLENY
                     SLDARFRLRGELPAHSQVVLVSITDACLAELGPWPWPRAKHAELLKILQREGAGSVGF
                     DLIFSETSALGATDDEIFAAAMRESGNVILAQTVGLRQTADPESLEPVTRFVVDRAVP
                     LLASASAGEGFIDMEAETMNTDGLIRNLLLQKTVDGRNHYLFGLALASHLLKQSIQPE
                     EHGVRLGERFLPYYFRRVNPGNQTVYSSFLLNYAGPNAHFRDVSYLDVLQGRFNKGIF
                     ASAAVIVGTRARGLSEDVKFSPFGAMAGMEIHANLLQNILDGRILHRLSPRQAGLLII
                     GLGLALGWVIW"
     gene            complement(1099..2196)
                     /locus_tag="PKO06_04225"
     CDS             complement(1099..2196)
                     /locus_tag="PKO06_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68881.1"
                     /translation="MTNDDLQLIKSNLEDADTLAENKNWAEAKAAYLKVLEDFDRIAA
                     ADPEASFTDEELTLKKKAQDQITIADAKLAQLHREQGIVALEAKDYARAVDELEEAIN
                     LAAETEVTFLEEVKSFLDKARILLRDQEIFTQISPSVKQGDDLRHAGAYAEAILEYQE
                     ALKSLGGMPENHRFVTYVKQSLRECKRALVRSYMRRSQRSADLGKYLRAYKVLQRAQL
                     VVADDDFTYRAFLLQIGERLASHLKPTDIDEASDIEAPEKWATAIKDYEEALTLYQSS
                     SRTDPFSPAFSRQNMYEDRFLSARRQLATLYRERGDKLRDKAEIEKALKNYKEALKLY
                     PRADKEFHATFSEIRKLRAQIGNPAPDAQTH"
     gene            complement(2193..4073)
                     /locus_tag="PKO06_04230"
     CDS             complement(2193..4073)
                     /locus_tag="PKO06_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68882.1"
                     /translation="MSRLALVLIVGLVVCSFGSLSASTSPTRLMEQAIKARSQGDTAG
                     AVQTLEEAVQVAVRKEQKMLALLMLGDCQLHAEQYEQAIDTYQNLIDLQPGMEEEAEA
                     RFRQAQAYLMLGQIAKTRELCRDLKKRFPKSEFARLATHLQKSAATAAGQSAAMPEAA
                     PAKSPVTEDDTPAAIKSEPVSGSKLAGRQKPQTSQIPRSEVEPVLEAAPVSGKPTAAN
                     TRVAALPKKPVMLTNKDLEEMNARSVGPSRRPQEDSPDADATEAESQDAVTKNETSES
                     EMSNDPEPVAKPRSTVSEDAEPVQVAKSGKFEEVEDIASALGQVSSAQPEPVEKTPVQ
                     EPAAAPVSSRPPDSLAALLTFPRLSRQESESLATEILKDQEELQLRSGGTPDAELLIR
                     LAGNTARFGEIKEACRLYDQLLRLHPGSRFVEEAYFEAIRLRVILRAYAAAQQWGTTF
                     LKSFPKSARRSALQKLLDHAAQQAGRKGAKRLETGGPADPEDALSRDPHYREGTRLRQ
                     EGRYALALEHLQRLTSSHGQNPRLWHELALIEIQMRRYPQAEKHVKKLLEIDPNHSEG
                     RSLLGYIHYQRREYQKAAGDYQKAREAKQGAKPEEGLTFFDSESAARRLHQADRRKGD
                     EP"
     gene            complement(4070..>5305)
                     /locus_tag="PKO06_04235"
     CDS             complement(4070..>5305)
                     /locus_tag="PKO06_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68883.1"
                     /translation="RQQLFRQVGTVVDLATDDVARSLAGVRDSMRLLARMPAFRQATQ
                     AGQATPALRMMQSQAGLLLVPCVQTEEALRMTRRQFHEDLVLSVETLAAEGRRLFYSV
                     GLWPAVEAEPVMSLGDGAGDSVSSLAMNTGGTDVRGNESSLVEQLIPVIEAAFNALVP
                     VTSLVDRVFPVGLPTLADPRAAENILRASLSDRDLIRSVAVKQLDGQEIISVTEIASS
                     FQMLSNWVKTATRAARSFYAGPVWFEEGLGRPIWNVAVPLRDEEREPFAMLSSQVDLG
                     FLGDMAQKTRLTATSYLLVVDDDGVVIAHPKASLVANQVNLTHSNRAVAEALRGEEGV
                     QELRLGDTTFIVGYRSLKKVDAAMLPAWGILYLTPLSEAIGSLIWDAANALVLIAVAL
                     YLIFWCAGILIEVIEEGEV"
BASE COUNT         1081 a         1633 c         1480 g         1111 t
ORIGIN      
        1 ttccagatca cccaccccag cgccaatcca agcccgatga tgagaaggcc ggcctgtcga
       61 ggcgagagac ggtgcagaat tcttccatcg agaatattct gcagcagatt cgcgtgaatc
      121 tccattcccg ccatggcccc gaacggcgag aatttcacgt cttcggaaag accgcgggcc
      181 cgcgtgccga caatgacagc ggcactggcg aatatccctt tattgaagcg gccctgaagc
      241 acatcgaggt aggacacgtc acgaaaatga gcgttcgggc cggcgtagtt gagcaggaac
      301 gaggaataga ccgtctgatt gccgggattc actcgacgga agtagtacgg aagaaatcgc
      361 tcacccagac gcaccccgtg ctcttcaggt tgaatcgact gtttcagcag atgcgaagcc
      421 agtgccagac cgaacagata gtgattgcga ccatcaaccg ttttctgcag cagcagattg
      481 cgaatcaacc catcggtgtt catcgtttcg gcttccatgt cgatgaagcc ttctccggct
      541 gaagctgagg ccaacaacgg aaccgcccga tcgaccacga atcgcgtcac cggttcgagt
      601 gactcgggat cagccgtctg gcgcaaacca acagtctgcg ccagaatcac gttgcctgat
      661 tcgcgcatgg ccgcggcaaa gatctcgtcg tccgtcgccc ccagagctga tgtttcgctg
      721 aagatcagat cgaacccgac actgccggcc ccttcccgct gcagaatctt caacagttcc
      781 gcatgtttgg cacgcggcca cggccacggc cccagctccg caagacaggc gtcagtaatc
      841 gatacgagta ctacctgaga atgtgcggga agttctcccc gaaggcgaaa tcgcgcatca
      901 agagaatagt tttcgagatt ggtgaacaat ccgaaataga aacagacagc gaagacacca
      961 ccgaacaaca gcgagatgaa acactcccag ctgtagcgat actgtccctg gccgcggaac
     1021 gatgcttcgg aggtcatgcg tggagaaaaa agaaaatgcc tcgaccccgg cagtcagacc
     1081 ggagtcgaag catggtgatc agtgagtctg ggcgtcggga gccgggttcc cgatctgggc
     1141 ccggagcttg cgaatctccg agaaagtcgc gtggaactct ttgtcagcgc gcgggtacag
     1201 cttcagcgct tccttgtagt tcttcagtgc cttctcgatc tcggccttgt cgcgaagctt
     1261 gtcaccgcgt tcgcgataca gggtcgccaa ttgtcgacgc gcactgagaa agcgatcttc
     1321 atacatattc tgccgcgaaa aagcaggact gaacgggtct gtacgagagc tcgactggta
     1381 caaagtcagc gcctcttcgt aatccttgat ggcagtcgcc cacttttccg gggcttcaat
     1441 gtcgctcgcc tcatcgatat cagtcggttt cagatgggac gccagccgtt caccaatctg
     1501 cagcagaaaa gcccgatagg tgaagtcgtc atcagccacg accaactgag cccgctgcaa
     1561 caccttgtag gcccgcagat acttgcccag atcggcagac cgctgactgc gacgcatgta
     1621 ggaacgcacc agcgcgcgct tgcactcgcg cagcgactgt ttcacgtagg taacgaaacg
     1681 atgattctcg ggcatgccac ccagactctt caacgcttcc tggtattcca gaatcgcttc
     1741 ggcataggct cccgcgtggc gcagatcatc gccctgtttc accgacggcg agatctgggt
     1801 gaagatttcc tgatcgcgca acagaatgcg tgccttatcg agaaatgact tcacttcttc
     1861 cagaaacgtc acttcggttt ctgcagcaag attgatggct tcttccagtt catcgacggc
     1921 ccgggcgtag tctttggcct cgagagccac gattccctgt tcacggtgca gctgagccag
     1981 tttcgcatca gcgatggtga tctggtcctg ggctttcttt ttcagcgtca gttcttcgtc
     2041 cgtgaacgaa gcttcgggat cggcagcggc aatacggtca aagtcttcga gaaccttgag
     2101 gtaggcagct ttcgcctcgg cccagttctt gttctcggca agcgtatccg cgtcttcgag
     2161 attggatttg atcaattgca gatcatcgtt cgtcatggct cgtctccttt gcgccggtct
     2221 gcctgatgca gacgccgggc ggctgattcg gaatcgaaaa acgtcaaacc ttcttctggc
     2281 ttcgcgccct gtttcgcttc gcgggctttc tggtaatcac ccgcagcttt ctggtactcg
     2341 cgacgctggt agtgaatata ccctaaaagg gaacgacctt cactatgatt cgggtcaatt
     2401 tccagcagtt ttttcacatg tttttcggcc tgggggtatc tgcgcatctg aatctcgatc
     2461 agagccagtt catgccagag gcgcggattc tgaccatgac tcgaggtcag tcgctgcaga
     2521 tgttcgagcg ccagcgcata gcggccttcc tggcgcagac gtgtgccttc tcggtaatgc
     2581 ggatcacggg aaagcgcatc ctcagggtct gcgggtccgc cggtttcgag gcgtttcgcc
     2641 cccttgcgcc cggcctgctg cgcggcatgg tccagcagtt tctgcagggc cgaccgccgc
     2701 gccgacttcg gaaacgactt gagaaacgtc gttccccact gctgtgccgc ggcatacgcg
     2761 cgcagaatca cgcgcaggcg aatcgcctcg aaataggctt cttcgacaaa gcgcgacccc
     2821 ggatgcaacc gcagaagctg gtcatacaac cgacaggcct ctttgatttc accaaacctg
     2881 gccgtgtttc ctgcaagcct gatcagcagc tccgcatcgg gagttccacc gctgcgtaac
     2941 tgcagttctt cctgatcttt cagtatttcg gtggccagcg actcgctctc ctggcgcgat
     3001 agccggggaa acgtcagcaa tgccgccaga ctgtcaggcg gccgggacga caccggggcc
     3061 gccgcaggct cctgaaccgg ggtcttctcg acgggttcgg gttgggcact gctcacctga
     3121 ccaagagccg aggcgatgtc ctcgacctcc tcgaactttc ctgattttgc gacctgtacc
     3181 ggttccgcgt cttccgacac cgtcgaacgg ggtttcgcca ccggctccgg atcattcgac
     3241 atttcagact cagaagtctc gttcttcgtg acagcgtctt gcgactccgc ttcagtcgcg
     3301 tctgcgtcag gggaatcttc ctgcgggcgt cgtgaaggcc cgacgctgcg ggcgttcatt
     3361 tcttcgaggt ccttgttggt cagcatgacc ggctttttcg gtagcgccgc cacacgagtg
     3421 ttcgcggctg tcggtttgcc cgagacaggt gccgcttcga gaaccggctc gacctcgctt
     3481 cgcggtatct gcgatgtctg cggtttctgc cgccctgcca gcttgctgcc acttaccggc
     3541 tctgacttga tcgcagcagg cgtgtcgtct tctgtgacag gagattttgc cggagcggct
     3601 tcgggcatgg ctgcgctctg tccggctgcc gttgccgccg acttctgcag atgcgtggcc
     3661 aaacgcgcaa actcactttt gggaaatcgc tttttgagat cacgacacag ctcgcgggtt
     3721 ttggcgatct gtccgagcat cagatatgcc tgagcctgcc ggaaccgcgc ctcggcttcc
     3781 tcttccatgc caggctgcag atcgatcagg ttctgatagg tgtcaatcgc ctgctcatac
     3841 tgttccgcat gcagctggca atcgcccagc ataagcagcg ccagcatctt ctgctctttg
     3901 cggaccgcca cctgcacggc ttcttccaaa gtctggaccg cccccgcggt gtcaccctgg
     3961 gatcgagcct tgatggcctg ttccattaag cgcgtcgggg atgtcgaggc agaaaggctg
     4021 ccgaaggaac acaccaccag acccacgatc aagaccagcg ccagacggct cacacctcgc
     4081 cctcctcaat gacttcgatc aggattccgg cacaccagaa aatcagatac agggccacgg
     4141 caatcagcac cagagcgttg gcggcatccc agatcagtga gccgatggct tcagagagcg
     4201 gcgtcagata caggattccc cacgccggca gcatggcggc atcgactttt ttgagcgagc
     4261 gatagcccac gatgaaggtc gtatctccga ggcgcaattc ctgtacccct tcttcgccgc
     4321 gcagcgcttc ggcgaccgct cgatttgaat gcgtcagatt gacctgattg gccaccagag
     4381 aggctttggg gtgcgcgatg accacgccgt cgtcatcgac gaccagcaga tatgaggtcg
     4441 ccgtcagtcg ggttttctga gccatgtcgc caaggaatcc gagatcgacc tgcgaggaca
     4501 gcatggcgaa gggctcgcgt tcttcgtcac gcaacggaac ggcaacgttc cagatggggc
     4561 gacccaaccc ttcctcgaac cagaccgggc ccgcataaaa cgatcgggcg gcacgagtcg
     4621 ccgtcttcac ccagttcgac agcatctgaa agctgctggc aatttccgtg acactgatga
     4681 tttcctgtcc atccaactgc ttcactgcaa ccgatctgat cagatcgcga tcactgagac
     4741 tggcgcgcag gatgttttcg gcggcacgcg gatcggcaag cgtcggcaac ccaacgggga
     4801 acactcgatc gaccaggctg gtcacgggga ccaaagcgtt gaacgccgct tcaatcacag
     4861 gaatcagctg ttcgaccagc gaggattcgt ttccgcgaac gtccgttccc ccggtgttca
     4921 tggccagcga cgagacggaa tcaccggcgc catcgcccaa cgacatcacc ggctcagctt
     4981 ccacagccgg ccacaggccg accgagtaga acaggcgacg cccctctgcg gccagggtct
     5041 cgaccgagag aaccaggtct tcgtggaact gccttctggt cattcgcagc gcttcttcgg
     5101 tttgcacgca cggcaccagc aacaggccag cctgactctg catcatgcgc agagcgggcg
     5161 tggcctgacc agcctgtgtc gcctgccgaa aggccggcat tcgcgccagc agccgcatgg
     5221 aatcccgcac tccagcgaga gagcgggcaa cgtcatcggt cgccaggtca accacggtac
     5281 ccacctgccg aaacaactgt tgtcg
//
LOCUS       DAORHD010000138         5295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16111_length_5295_cov_3.602099, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000138 DAORHD010000000
VERSION     DAORHD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16111_length_5295_cov_3.602099"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKO06_04240"
     CDS             <1..309
                     /locus_tag="PKO06_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68884.1"
                     /translation="LLDWAEAAGEATAAIQRVLWLGVGSHERPPDDVRRLLAEMPDVE
                     PLIELWGELAKAGDLEGLTVMRWLAECGRLSSDADLAKGDQMKGKRGVLESNTNVEPE
                     "
     gene            362..550
                     /locus_tag="PKO06_04245"
     CDS             362..550
                     /locus_tag="PKO06_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68885.1"
                     /translation="MKGIMPIPGKDPTWFWFLVLTYGICKAWKIWMGEEEEPKSGSPQ
                     ASHTSEAEERGETESERK"
     gene            1285..2004
                     /locus_tag="PKO06_04250"
     CDS             1285..2004
                     /locus_tag="PKO06_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68886.1"
                     /translation="MDIELGSHDPDKKNNVRLSMNLNRWHKICERLTKQANEAQTFVE
                     RTFGETHVPTYTGGQQTQKLEELASRGLLALELHGALLRARQTVRAAIGRANDEWGIT
                     ERLVQVAAINERLNCLIKTFTRQHPNRVPVEEIERLFARADAARQTLQCASNADLFGE
                     LTKEVDPDDLYKSHRTYERVLQNKGADVQLMTAEEAAEIEKEILRLRGLKYRLQDEIA
                     EANLGLVELDLPGQVVARVLG"
     gene            2084..2791
                     /locus_tag="PKO06_04255"
     CDS             2084..2791
                     /locus_tag="PKO06_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68887.1"
                     /translation="MFQTSDVPHRTCTYAMSRWHKYAERLDRQIAEQVHLIDYAFGTM
                     TVPTYMGPTQIDTLRRLSERGRQALHMHRRLLAAKALIRSALSREGVRVGTSEKLARI
                     NTIEQQLSLLIRVFCRQHPSRLEIGEIERFFARMEAARRPVEFATTTRNSWLEEEGQG
                     SAADPEDTMWAQKEYSGSKGVDVQLLDRAQIIRLEQRILALRAEKFRLQDEIAAANTK
                     PLTAELPEEMIALVTGG"
     gene            3118..4299
                     /locus_tag="PKO06_04260"
     CDS             3118..4299
                     /locus_tag="PKO06_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68888.1"
                     /translation="MAHWVKDLFRLLKRPYSEDLIAELEARISALEEKMRDGGFIYRV
                     TEGLADQYELDALNLKLFELKEHVPEKFFRAEAKTVWATNEYRVEPAAAKRQTWEIIV
                     PGMLVKLVSGQRTCAQGGFFKVEVLGAKRVISVRRRELERFGNHELPEAVIEQNRTRW
                     RERVSRFNRFQKNEEYLLVGDQVMLESDARIRLACGARELYRAMQRHGLNEVVQGHIV
                     GLVRLMRQMRGHPRGHDLEPIIELVAAELPDRAALEGAMARIIEWAGADVTLDPPRSK
                     TDQEWPPRQKGDLPFIDAPVFKRPRLAEMYAAAGLFEPGFTYTRAQVEKLVSGCASSG
                     APNDPRFPLEVLIRNMVDIGYLERDVRGLRYWLRIGPWCESMPANAWPEPETRKEHLA
                     T"
     gene            4410..>5295
                     /locus_tag="PKO06_04265"
     CDS             4410..>5295
                     /locus_tag="PKO06_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68889.1"
                     /translation="MKRFWLLVTFFTLVVLPAQVGMMLRESCNPDLAESYLATAQMQI
                     DKGNEDGALAIWDFLATHDLDETGVAMASYTAVIAKQNQISAKVYRGVTGALKGYVLG
                     EVTDPASLAGCTAGDLMVWGDVRDLIKNGYVYCTGGETDTLIVALSSIGLATTLAPHL
                     DVGISVAKTFARFMSKTLRAGLELLVTEAKATGKLDKVTGLLSSLGTTYRRIGMGTLD
                     LVQFSKDSAMLTRLAGIVESYGRAGYGAIMLGGKSAVTFLGVALDAGVKLTGAAGKRI
                     MRFGLQYPKWGARALKVAK"
BASE COUNT         1150 a         1420 c         1728 g          997 t
ORIGIN      
        1 ctgcttgatt gggccgaagc ggccggcgag gcgaccgccg ccatacagcg ggtgctgtgg
       61 ttgggtgtcg gttctcacga gaggccgcct gacgatgtgc gccgcctgct cgcggagatg
      121 cctgatgtgg agccgctcat cgagctctgg ggcgaactgg ccaaagccgg cgacctcgag
      181 ggtctgaccg tcatgcgctg gcttgccgag tgcggtcgtc tgtcgtctga cgccgatctc
      241 gccaaaggcg accagatgaa gggcaagagg ggcgttcttg aatcgaacac gaatgttgaa
      301 ccggagtgaa caaagatgct tttcgtaagt gacgaaccga acaaacccac aggaggttgc
      361 aatgaaagga atcatgccga taccggggaa agaccccacg tggttctggt ttctggtgct
      421 gacctacggg atctgcaagg cctggaagat ctggatgggc gaggaggagg agccgaagag
      481 tgggtcacca caggcttctc atacgtcgga ggccgaagaa aggggagaga cggagagtga
      541 gcggaagtga ttgcagacag gcccacggga acaggcaggg cggtgctggg cgactctaca
      601 gtagaagggt tgatatgtcc gagttggttt gttgcaaaat gcagatcgga aaagcgtgag
      661 agatgaaaat aaagtgaaaa tgatcttgac agcgagggcg aatttcgata tgatggtaat
      721 gaaagcgaaa aatccgcgga ggttgtcgag agcgtatgta agccgtcgtc gtcgaagtct
      781 tcgccccgaa tagtcacaca ttggggctat acgaaaagca ggtggttctg cgcttgcaaa
      841 acggaagaat cgacgacggc ggcttcattt ttgtcgctct ttgacaactt catccggtcc
      901 gcggagggag ggggggtaag gctcctcccg gccaggtctg cgttccgcaa taccctggcc
      961 atggtcacct tccgtgcgac cgagacactc tcaccacgag ggcggttcgc cagaccaacc
     1021 cggacaccga cacatccccg cgccgaccct ttctttccga cggatgcgcc tatcggcgct
     1081 gacggctttc ggccgtttgc accgacaggc actctctctc taactccttt tcggcctacg
     1141 attgcggggc gtgtgaccga tcgctcgccg tacatctccg cgggggtgta acgagccggc
     1201 gaggctcatc gagaccctcg catttcgtac gcgtgagggc caagaaaggc tctcgaacat
     1261 tcttcaccaa cacaggaggt ggttatggac atcgaattgg gttcacatga cccggacaaa
     1321 aagaataacg tcaggctgtc gatgaacctg aaccgctggc acaagatctg cgaacggctg
     1381 acgaagcagg cgaatgaggc gcagaccttc gtcgagcgca cgttcgggga aacccacgtg
     1441 ccgacctata ccgggggaca gcagacgcag aagctggagg agctggcgag ccgagggctg
     1501 ctggccctcg aactgcacgg ggccctgctt agagcacgtc agacagtgcg tgccgccatc
     1561 gggcgcgcca acgatgaatg gggcattaca gaacgtctgg tgcaggtggc cgccatcaac
     1621 gagcggctga actgtttgat caagaccttc acgcgacaac accccaaccg agtaccggtc
     1681 gaagaaatcg aacgcctgtt cgcacgggcc gacgcggcac gccaaactct gcaatgcgcg
     1741 tcaaacgcag acttattcgg tgaattgaca aaggaagtcg accctgacga cttgtacaaa
     1801 tcgcacagaa cgtatgagcg ggttctgcag aacaagggtg ctgatgttca gctcatgacc
     1861 gctgaggaag ccgctgagat cgaaaaggaa atactgcgcc tgcgcgggct caaataccgc
     1921 ctgcaagacg agatcgcgga agcgaatctg gggctcgtcg aactcgacct gccgggccag
     1981 gtcgttgctc gcgtgctcgg ctgaaccgac acccacgcga caagacgcgg ccgccgcaca
     2041 gagcggattg cggcggtcag accgaaccca aaggaggaat gccatgttcc aaacatcaga
     2101 tgttccgcat cgtacgtgca cgtatgcgat gagccgctgg cacaagtatg ccgagcggct
     2161 cgacaggcag attgccgagc aggtgcatct gatcgactac gcgttcggca cgatgaccgt
     2221 gccgacctat atggggccga cgcagatcga caccctgcgc cgtctgagcg agcgcggacg
     2281 tcaggccctg cacatgcacc gccgcctgct ggccgcgaaa gctctgatca gatcggcgct
     2341 cagtcgtgaa ggtgtgagag taggcacttc cgagaagctg gcgcgcatca acaccatcga
     2401 acagcagttg tcgctgttga ttcgggtgtt ctgccgacag catccgagcc gtctggagat
     2461 cggcgagatc gagcggttct tcgcgcgcat ggaggccgct cggcgtccgg tggagttcgc
     2521 gaccacgacg cgaaatagtt ggcttgaaga agaaggacag gggagtgccg ctgaccctga
     2581 agacacgatg tgggcgcaga aagaatactc cggctcgaaa ggggtcgacg tgcagttgct
     2641 cgacagggcc cagatcattc gactggaaca acgcattctc gcgttgcggg ctgagaagtt
     2701 ccgtctgcag gacgagatcg cggcggcgaa tacgaagccc ttgacggcgg aactgccgga
     2761 agagatgatc gctctcgtca ccggcgggta acgtcgagtg agccgcttgg cagagccagc
     2821 cgggcggcca ggagatcagg ctcaggcaat atccggggtc aaacatcggc tgtcatgcgg
     2881 cgctgtgcct tcgccgactg agcggaaggt gcgtctgtcg tgacagtacc gatgaaggtt
     2941 gcatgttgtc cgttgtttgt gacccttccg caaggaagga aagttgcaga tgccgttgag
     3001 ggaaacacct gccccggagt gttttcctgt gtgtggcacc ctgcgggctt cggctcgcag
     3061 ggcccgccga ttttttatcg ctggattgtt actcttatca agagaggaga aattcgtatg
     3121 gctcactggg tgaaagactt gttcagattg ttaaagcgtc cgtattcgga agacctgata
     3181 gcggagctgg aagcccgaat cagtgcgctg gaagaaaaaa tgcgggatgg tggtttcatc
     3241 taccgcgtca ccgagggtct ggccgatcag tacgaactcg atgcgctcaa cctcaagctg
     3301 tttgagctga aggaacacgt tccagaaaag tttttccggg ctgaagcgaa aaccgtctgg
     3361 gcgacgaacg aataccgagt ggaacccgca gccgccaaac gccagacgtg ggagatcatt
     3421 gttcccggaa tgctggtgaa gctcgtgtcc ggacaacgca catgcgcaca aggcgggttt
     3481 ttcaaagtgg aagtgctcgg cgcgaagcgg gtcatcagcg tgaggcgtcg tgaactcgaa
     3541 cggttcggca accatgagct gccagaggcg gtgatcgaac agaatcgtac tcgttggcga
     3601 gagcgggtaa gccgattcaa tcgatttcag aagaacgagg aatacctgct ggtcggtgac
     3661 caggtgatgc tcgagtcaga tgcccgaatc aggctggcct gtggcgcgcg tgaactgtat
     3721 cgcgccatgc agcggcacgg tctgaacgag gtggtgcagg ggcacatcgt tggtctggtg
     3781 cgacttatgc ggcaaatgcg ggggcatcca cgcgggcatg atctggagcc gatcattgag
     3841 ttggtggccg ccgaactgcc tgaccgggcc gcgctggagg gggcgatggc acgcatcatc
     3901 gagtgggccg gagcagatgt cacccttgac ccgccgcgat caaaaacgga tcaggagtgg
     3961 ccgccccgcc agaagggaga cctgcccttc atcgatgcac cggtgttcaa acgtccgcgt
     4021 ctggccgaga tgtatgccgc cgccgggctg ttcgagcccg ggttcacgta tacccgcgct
     4081 caggtcgaga agctcgtgtc agggtgtgcg tcgagcggcg cgccgaacga cccgcggttt
     4141 ccgctcgagg tgctcattcg caacatggtc gacatcggat acctcgagcg tgatgtgcgc
     4201 ggtttgcgat actggctgcg tattggcccc tggtgcgaat ccatgccggc caacgcctgg
     4261 cctgaaccgg aaacccgcaa ggagcacctc gcgacgtgag cggtgagaag acaagtgcgc
     4321 tgcagttgtg gtacacgtga agaagggcgc atcacctgcc tggatgattc gacagaacga
     4381 acagggagcg ctggagaggg aggcatagca tgaaacgttt ctggttgctg gtcacgtttt
     4441 ttacgctggt ggtgttgccg gcgcaggtgg gcatgatgct gcgcgagtcg tgtaacccgg
     4501 atctggcgga gtcctatctt gccacagcgc agatgcagat agacaagggg aacgaggacg
     4561 gtgcactcgc catctgggac tttctggcca cgcacgatct cgacgagacg ggcgtcgcga
     4621 tggcttcgta cacggcggtg atcgccaagc agaatcagat ctcggccaag gtgtatcgtg
     4681 gcgtcaccgg ggctctgaag gggtatgtgt tgggtgaagt gactgacccg gcttcgctgg
     4741 ctggctgtac cgccggtgat ctgatggtct ggggcgatgt tcgcgatctg attaagaatg
     4801 ggtatgtata ctgcaccggc ggcgaaaccg acacgctgat cgtcgcactt tcgagtatcg
     4861 gtctggcgac gaccctggcc ccgcatcttg acgtaggcat ctcggtggcg aaaacctttg
     4921 cccgcttcat gagcaagacc ttacgtgccg gcctggaact gctggtgacc gaggccaagg
     4981 caaccggcaa actggacaaa gttaccgggc ttctcagttc actgggaacg acgtatcgtc
     5041 ggatcggtat gggaactctt gacctggtac agttctcgaa agactcggcc atgctgacgc
     5101 gtctggctgg tatcgttgaa agttacgggc gtgccggtta cggcgcaatc atgctcggcg
     5161 gaaaaagcgc ggtcaccttt ctcggcgtcg cgctcgatgc cggggtgaaa ctcaccggtg
     5221 ccgccggtaa gcgcatcatg cgctttggcc tgcaataccc gaagtggggc gcgcgtgcgc
     5281 tgaaggtcgc taaaa
//
LOCUS       DAORHD010000139         5284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16156_length_5284_cov_3.655957, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000139 DAORHD010000000
VERSION     DAORHD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16156_length_5284_cov_3.655957"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PKO06_04270"
     CDS             complement(<1..1127)
                     /locus_tag="PKO06_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68890.1"
                     /translation="MPLSKRDKEFRFTKISYLDFKFIEPDRVMTSLFMRLAHQGLSSR
                     LRKKTEKTLEDFVGEFCSNPEKFVDFHQYRDVVSRWVETDLLDIVNRGKANQAIAAPR
                     PLHGFTYRFRNPNHSRPYGADQQLYEMLAQARTPWSQGCLDQLKAFCFEGYDPVTGKP
                     LPNQVLDVETRALLHLSEQVEDAPDTRGGRDSHPPPCIGSADLLADDIKRLLFYQRFI
                     PRSVMIDYLKILLFFHLGMAHLRLLKMLPLLVRSHGKAPLCEKSTCPMNPQKMIDPHG
                     ACPFQIGLFVDAAGTTHTRFLAERSADVHYRRIPPFIRAYFTARKLDEFGEDLLKRQK
                     TARPISGEFSLFEVLRFLDSTFAGEQKIFFGQRISAITQDTG"
     gene            complement(1118..5278)
                     /locus_tag="PKO06_04275"
     CDS             complement(1118..5278)
                     /locus_tag="PKO06_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV68891.1"
                     /translation="MGRVIPIGEPVNDAERQTIGFLRDHLPDSYLILHNFELGRDGER
                     FEIDLAIIAPHAVYLVDIKSTHGTIDVHGPHWYPDGRAPFSSPLLKLRHHARALKGVI
                     TASHPHKPELERVYVTAAVILSAPTARLLDPENRDGPDVTTLARASSFFQQADRIPAI
                     FDRNLLSLQNIILKAVQGAAKRRVGPLRFGNWEISEKLGGTDSFTEYRAYNIFAGRAA
                     GSVRLRRYQADPYLPIEQREAQQRHISNAYQAINRLPSHPSIIGARDFFGSEAGDSYI
                     LVTEDAPGQALRLHLDQPARVLTFDQKRRIAADLLAALAHAHAHQVVHRNLTPSCILV
                     RPDGGIALGGFDFARVGQDRSHSIAREIIEDLDERYAAPEVHREPQAASPASDVFSAG
                     LILYELFTGERPFQNQSEVFSQGGVFAQKPSVLRSELNAAFDEWIQMLCRFEPGSRPS
                     SSTALETFYQLVHPEQTPASEPAATPTPEPDYFNLPPNFPLSRKFVVQKRLGKPGSFG
                     AVYKVIDTLGDVARAVKLILRDRTSVIERLKTEYRALVTLPEHPHVVKVFDADFLPGS
                     QVPFIVFEYVDGLDVGEMIDEKCFSADDALLFARQTLAGLGHLHRNNIRHCDIKPRNL
                     LWTGKGVKIIDFNVSVRTIAGNSHGGGTKRYLPPDLELSGEPTDLELRDRDLYAFGLT
                     FFEVVTGRYPWDASIPPAGQPAPDPRTFSDCANLHPEVVAFLAKALASKRADRFHSTE
                     EMEAALSRLASIQTSSSGALAGTTDTESLIQRMDLGEANANPFVDYLLTLFSQSTRSN
                     RGTRGLDELGKKIYVETALDRELAPAVLAGQFDLVIITGNAGDGKTAFLQQMEDLARH
                     KGGTIEEAFLNGSRFQLEGRWFQCLYDGSQDEGDKTSDDVLLAFFRSFAGAEIPRQSA
                     NETRLIAINEGRLVDFLLSAGDRFARLKQIVTQGLATGVPEERIAVVNLNLRSIVGDS
                     VLGGQNGVGSILRRLMQRLVQPKLWEPCAACDLADRCYVLHNVHTFQDSTAGVKVLER
                     LETLFTLTHLRGQLHVTMRDLRSALAFTLAGTRSCREIHVLYRSGSRDEVLAGFYFNS
                     WMGGDRPISDRLIAVLAELDIGKAAEPQLDRRLDFLAPDESRHLFTFDRRARYDRELL
                     ESTFADLPRDCTSSRVVEVSAAHRRFVAMCRRRDYFERRDGGWRQLLPYHSADRMLTM
                     IERRLIEPKLVSEILDGINRGEGVFHPSQLGGGLALQVRRVDNGTIRSYRLFPANRFS
                     IALQDQSERAKFVEHVPRGLLLRYATEQTTNAELEINLDIFEMLHRLNAGYRPSLEEE
                     QGYYLSLTVFKNVLAAAPYQEVLLTSTGHEFFVIRNLEADRLEMHRLERPKSVTQKRE
                     NGESSPCR"
BASE COUNT         1140 a         1419 c         1604 g         1121 t
ORIGIN      
        1 cctgtatctt gcgtgatagc gctgatccgt tgtccgaaaa agatcttctg ctcgccggca
       61 aaggtggaat ccaggaaacg gagaacctcg aagagcgaaa actcaccaga tatcggtctg
      121 gccgttttct gccgttttaa caggtcttct ccgaactcat cgagtttgcg tgcggtgaaa
      181 tacgctctga tgaacggagg aatccgacga taatgcacat cagcactacg ttcagcgagg
      241 aaccgagtgt gcgtggtgcc tgccgcatca acaaagagcc cgatttgaaa tggacaggct
      301 ccgtgcgggt cgatcatctt ctgtggattc atcgggcatg tactcttttc gcaaagcgga
      361 gctttcccgt gtgagcgaac cagcagagga agcatcttga gcaggcggag gtgagccatc
      421 cccaggtgaa agaacaacag aatcttgaga tagtcgatca tgaccgatcg tggaatgaag
      481 cgctgataga acagcaggcg tttgatatcg tcggcgagca gatcagccga accgatacaa
      541 ggcggtgggt gtgaatccct tccgccgcgc gtgtcgggcg catcttccac ctgttcgctt
      601 agatgaagaa gcgcacgggt ttcgacatcg agcacctggt tggggagcgg ctttccggta
      661 accgggtcgt agccttcgaa acagaaggct ttcagttggt cgaggcaccc ttggctccac
      721 ggtgttcgag cctgtgccag catttcatac aactgctgat cagccccata cggtcgagaa
      781 tgattggggt tgcgaaatcg ataggtgaac ccgtgcaacg gtctgggggc cgctatggcc
      841 tggttcgctt tgcctcggtt gacgatgtcg agcagatcgg tctcgaccca ccgagatacc
      901 acgtcgcggt attgatgaaa atcgacgaac ttttccgggt tgctgcaaaa ctccccgacg
      961 aaatcctcga gagtcttctc agttttcttg cgcaggcggc ttgaaagccc ttgatgggca
     1021 agccgcataa agagagaggt catgacgcga tcaggctcga tgaacttgaa atcgagataa
     1081 ctgatcttgg tgaagcgaaa ctccttgtcg cgtttgctca acggcatggc gaactctccc
     1141 cgttttcgcg tttttgggtg acgctcttcg ggcgctccaa gcggtgcatc tccaggcgat
     1201 cggcttccag attccgaatg acgaaaaact catgccccgt cgatgttaag agcacttcct
     1261 gatatggggc ggccgccaat acgttcttga aaaccgtcag actgaggtaa taaccttgct
     1321 cttcttccag actcgggcgg taacctgcat tgagacggtg gagcatctcg aagatgtcga
     1381 gattgatctc gagttcggca ttggttgtct gctcggttgc ataccgcaac agcagaccgc
     1441 gtggaacgtg ttctacgaac ttggctcgtt ccgattggtc ttgcagggcg atcgaaaagc
     1501 ggttggccgg aaacaaacgg tagcttctga tggtgccatt gtctacccgc ctgacttgaa
     1561 gagccagccc gccgcccagc tgggagggat gaaacacccc ctcgcctcga ttgataccgt
     1621 ccagaatttc actcaccagc ttcggttcga tcaggcggcg ttcgatcatg gttaacatcc
     1681 ggtccgctga gtggtagggc agaagctgac gccacccccc gtcgcgtcgt tcaaagtagt
     1741 ctcgacggcg gcacatggcg acgaaacgac gatgggccgc tgaaacctca acgactcgcg
     1801 agcttgtaca atcgcggggc aggtcggcga aggttgattc tagcaactca cggtcatatc
     1861 gagcgcgccg gtcgaacgtg aacaaatgcc gtgattcgtc cggggcaaga aaatccagac
     1921 gacggtctag ttgcggttct gcggctttgc caatgtcgag ttcggccaga acggcaatga
     1981 gccgatcgct gatcggtcga tcaccaccca tccagctgtt gaaatagaag ccggcgagaa
     2041 cctcatcacg cgacccgcta cgatacaaaa cgtgaatctc gcgacaagag cgggttcctg
     2101 ccagggtgaa cgccagcgcg gagcggaggt cgcgcatggt gacatgcagc tgtcctctca
     2161 gatgagtcag ggtgaaaagt gtttccaacc gctccaacac tttcacgcca gccgtcgaat
     2221 cctgaaaggt gtgaacattg tgcaggacat aacagcgatc agccagatca cacgcggcgc
     2281 atggttccca cagcttgggc tgaaccaatc gttgcatcaa tcgccgaaga atgcttccca
     2341 cgccgttctg cccgccgagg acgctatcac cgacaatact gcgtagattg agattaacca
     2401 cggcgattcg ctcttccggc actcccgtgg ccaaaccctg cgtgacgatc tgcttgagcc
     2461 gagcgaagcg atcaccggca ctgagaagaa agtcgacgag ccgcccctcg ttgatggcta
     2521 tgaggcgagt ttcgtttgcc gactgtctcg ggatttcagc gccggcaaac gagcgaaaga
     2581 atgccaggag cacatcgtca ctggtcttgt cgccttcgtc ctgactgccg tcatagaggc
     2641 actgaaacca ccggccctcg agttgaaatc gactgccgtt cagaaaggct tcctcgatgg
     2701 ttccgccctt atgacgggcg aggtcttcca tttgctgaag aaaggcggtt ttgccatcac
     2761 cggcattccc ggtgatgatt accagatcga attgtccggc taatacggcc ggtgccagct
     2821 cgcgatcgag ggcggtttcg acgtaaatct ttttcccaag ctcatcgagc ccgcgagtac
     2881 cgcgattcga acgggtactc tgactgaaca gggtcaggag atagtcgacg aaggggttcg
     2941 cgttcgcttc acccaaatcc atacgctgga tcagtgattc cgtgtcagtg gttcccgcca
     3001 gggcacccga agagctggtt tgaatcgagg caagtcggct cagtgcggct tccatctctt
     3061 ccgtcgagtg aaagcggtcg gcgcgctttg aggcgagcgc tttcgccaga aaggcaacca
     3121 cttcgggatg caaattggcg cagtcgctga aagtgcgcgg gtctggtgcg ggttggccag
     3181 cgggtggtat ggaagcgtcc cagggataac gaccggtgac cacctcgaaa aaagtcaacc
     3241 cgaaggcata gagatcgcgg tctcgcagtt ccagatcggt cggttcaccc gacagttcga
     3301 gatccggtgg cagatagcgt ttggttccgc ctccgtgact gtttccggcg atggttctga
     3361 ccgaaacgtt gaaatcgatg atcttcacgc ctttgccggt ccacaagaga tttcgcggtt
     3421 tgatatcgca atgacggatg ttgttgcggt gcaagtgacc caacccggcc agcgtctgtc
     3481 gggcaaacag cagggcatcg tccgcagaga agcatttctc gtcgatcatt tcgccgacat
     3541 caagaccgtc gacatactcg aagacgatga agggaacctg gcttccgggc aggaagtcgg
     3601 catcgaaaac cttgaccaca tgcgggtgtt cgggaagggt gaccagagcg cggtactctg
     3661 ttttgagccg ttcgatcacc gacgttcggt cgcgtaagat cagcttgacg gcacgggcca
     3721 cgtcgcccag cgtgtcgatg actttgtaga cggcgccgaa cgaacccggc ttgccaagcc
     3781 gtttctggac cacgaatttc cgcgagagcg gaaagttggg cggaagattg aaatagtcag
     3841 gctctggtgt cggtgttgcc gctggttcgc ttgccggtgt ctgctctggg tgtacaagtt
     3901 gataaaatgt ttcaagagcg gtagatgaag agggacgtga gcctggttca aatcggcaga
     3961 gcatctggat ccactcatcg aatgcggcgt tcagctcgct gcgcaaaacg gaaggctttt
     4021 gcgcgaacac tccgccttga ctgaacacct cgctttggtt ctgaaacggg cgctcgccgg
     4081 tgaataactc gtacaagatc aacccggcag aaaatacatc agaagccggt gaggccgcct
     4141 gtggttcgcg atgaacctcc ggagcggcat atcgttcgtc aagatcttcg atgatctcgc
     4201 gagcgatcga gtgtgaacgg tcttgcccga cgcgggcgaa atcgaaaccg cccagagcga
     4261 tgccgccatc ggggcgaacc agaatacacg atggtgtgag gttgcgatgc acgacctgat
     4321 gggcgtgcgc atgcgcgagc gcggcgagca gatctgcggc gatgcgccgt ttttgatcga
     4381 aggtcagtac ccgtgccggt tgatcgagat gcagacgcag agcttgtccg ggggcgtctt
     4441 cggtgacgag gatatagctg tcgcctgctt cagaaccgaa aaagtcgcga gccccgatga
     4501 tcgaggggtg agacggcagg cggttgatag cctggtaggc gttggagatg tgcctctgct
     4561 gggcctcgcg ttgctcgatg ggcagatacg ggtcggcttg atagcggcga agtctgaccg
     4621 agccggccgc gcgcccggcg aagatgttat aagcgcgata ctcggtgaag ctatctgtgc
     4681 cgcctagttt ttcactgatc tcccagttgc cgaatcggag cggcccgacc cgccgttttg
     4741 ccgcgccctg aacggctttt agaatgatgt tctgcaggga gaggagattg cggtcaaaga
     4801 tcgcgggaat acggtcggct tgttgaaaga agctcgaggc cctggccagc gtggtgacat
     4861 ccggtccgtc acggttttcg gggtcgagaa gccgagcggt cggcgcggac aaaatcaccg
     4921 cggcggtgac gtacacccgt tcgagttcgg gcttgtgcgg gtgactggcg gttatgacgc
     4981 ctttcagcgc gcgggcgtgg tggcgaagct tcagcagggg tgaagaaaac ggggcgcgtc
     5041 cgtctgggta ccagtgcggg ccgtgaacat cgatggtgcc gtgcgtgctt ttgatgtcga
     5101 cgaggtagac ggcatggggg gcgatgatcg ccaggtcgat ctcgaatcgc tcaccgtcac
     5161 gaccgagttc gaagttgtgc agtatcaggt agctgtcagg cagatgatct cgcaagaaac
     5221 cgatcgtctg ccgttcggca tcgttaaccg gctctccgat cggaatcacc cgccccatca
     5281 gacc
//
LOCUS       DAORHD010000140         5279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16191_length_5279_cov_3.728560, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000140 DAORHD010000000
VERSION     DAORHD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16191_length_5279_cov_3.728560"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PKO06_04280"
     CDS             <1..685
                     /locus_tag="PKO06_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV68892.1"
                     /translation="AIAGAFLFTGASLQQVGLQYTTAGKAGFITGLYVVLVPLIGRLF
                     GHRTDLFTWSGAILATAGLYFLSVTEQFTIAFGDLLELIGAVFWAGHVLLVGRYASRF
                     EPVAFSVGQFSVCSVLSFVTALKLEVISTTGLMGAAIPIAYGGFISVGIGYTLQVVAQ
                     RDAPPAHAAILLSLESVFAALTGWLLLQELMSGRAIFGCVLMLAGMLLAQFGPSPEEK
                     PVLIGTTGN"
     gene            complement(776..1480)
                     /locus_tag="PKO06_04285"
     CDS             complement(776..1480)
                     /locus_tag="PKO06_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68893.1"
                     /translation="MKTRGRGYVSVLIGILVVIVSVSGALWAQAATGSEPVVPGTTAV
                     TPEMVNQLGVQIQDLMKNTALASNPAGLEILKNVNGQIENLGKNLQNMTQEMTKNTLQ
                     TVNNNLEKVLQSVNEQTRQTVQKAVSQTMTQVNTMIQSAMQQATQMTNQLVNQAMDQA
                     QKDVDAAMKEADKALADGLKAGDDAMKEALKGIGDSTSNPVPVTGSGDSTEPAEPIDP
                     PDPPEPPEIPEVPETE"
     gene            1600..2448
                     /locus_tag="PKO06_04290"
     CDS             1600..2448
                     /locus_tag="PKO06_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68894.1"
                     /translation="MPLKTIFRSRRSWYLALILLSLSASCALAGNPASCAADPWPAIG
                     SAITATFAPIKPGMSNFNLDFSLWSGSSLLSTGTIGISNSKTVWFGLRSPQSSFRYLS
                     QGASISCLLVTPSDTVLFHAKPGEYSPFPLVRIDKEASGGFRLDLTVNYRTFNGSEPA
                     EFSVHPETAAYLTAKLRDRYPMVRESAEEYRLRASENASDSVILEKGKDGIGGFVAHF
                     PMEGQPPLKLQVSPLNVTGPFPTPPDNWIPAGGRTPSSGLDATVTFCKGFYSILSEIL
                     QPATTK"
     gene            complement(2515..4665)
                     /locus_tag="PKO06_04295"
     CDS             complement(2515..4665)
                     /locus_tag="PKO06_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNV68895.1"
                     /translation="MNRLMSLRLLLALCLLVAFGGVVDRLSADILTLNNGDELFGTVK
                     NLDAAGIQFERNGTVASYALSQVLKLEFETLKMPAGEDTVERISDPLLRELMKDVPKA
                     EAFPQASRLDVLDEEVYEMKDAGTWEYTARQVFVPFKEVARDDANHSFGYFPDLEKAE
                     VLFGRSIMAPESGLMGLVGGKPGSVRYVSDHTIADEADFGTVPLYQRRHTVKFAIPEV
                     TMGSIVDRQYRIRRFKADPLKPFLAEKLFRGREPARLMRLIVRAPRSLDLVFQAIDPQ
                     KVIKVETIEKDQMQEWRFEACNTEGLIDEPLMPELERLAPRVVIGLKNTWKGVASEYE
                     ACLTPLRTQALADPALETIVTRVCSGTGDLSEKARRLFTFMARDIGDVDVFPDWYSWQ
                     PHPVAQTLQAKQANQVDRAFLYHCLLLKAGVPNQVLPVRWRMLGEVASEVPSLMQMSG
                     LVVEIDSQAASFPMGLPVVRDLGPEVPPSFLQGASARRLSDGSVATVPYVPAKEEGNR
                     SIHEGEITADGTLKMKTTLQPRGGTAGDWRGMRDYTKLEQEKYLESMLHEMIPGARLL
                     SFTLANLDDTASTPLLTWEWEVPAYALRSGDDLLVIPLPVNRKAYSAAAVGPATRRFP
                     LTWDVLRRDEQTIALRLPDGYTVYALPEGGYASARGMMYHSRFGFSVNRVLFTDTLER
                     HVTDLDASEYVEYKKLIEGRSQTSDQWIIVKRAR"
     gene            complement(4662..>5279)
                     /locus_tag="PKO06_04300"
     CDS             complement(4662..>5279)
                     /locus_tag="PKO06_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68896.1"
                     /translation="SGLLQAETTKRYAGDYEAWARASWKNIKEEERGLYLSQRINKYG
                     KGAELATYTLVNLQDIEFPFSYSFQYRIPQYPREVADLMVFAVPGLLDYLTFPEAGLA
                     TRVYPIEYDTTEMQKDEGTVSLGEGLTIRSLPEPVRVDTPWFTFIGEYQREGDRAITY
                     RFAFRRLAKRVELKDYPEFKRQIERVQKCARDRIFLMKATQGGKP"
BASE COUNT         1014 a         1678 c         1402 g         1185 t
ORIGIN      
        1 tgccattgcc ggagcgtttc tgttcaccgg ggcttcgctg cagcaggtgg gtctgcaata
       61 caccaccgcc ggcaaagccg gtttcatcac cgggctctac gtcgtcctgg ttccgctcat
      121 cggtcgactg tttggtcatc gaaccgatct cttcacctgg tccggagcta ttcttgccac
      181 cgccgggttg tactttctga gcgttaccga acagttcacc atcgcgttcg gcgatcttct
      241 ggaactcatt ggcgcggtct tctgggctgg tcacgtgctt ctcgtcgggc gttacgccag
      301 ccgtttcgaa cccgtggcat tctctgtcgg acagttcagc gtctgttcgg ttctgagttt
      361 cgtaaccgct ctcaagctcg aggtcatcag cacaaccggc ctgatgggag ccgccatccc
      421 catcgcgtat ggcggcttca tctcggtcgg gatcggctac acgcttcagg tcgttgccca
      481 gcgagacgcc ccaccggccc atgccgcaat tctgctcagt ctcgagtcgg tttttgcggc
      541 tctcaccggc tggctgctgt tgcaggaact gatgtccgga cgcgcgatct tcggatgtgt
      601 cctgatgctt gccggaatgc ttctcgccca gttcggtcct tcgccggaag aaaaaccggt
      661 gctgatcggc accaccggaa attagcacga aaccgccacg actccacact gcccgacccg
      721 cgccttgccg gcgcgggtcg gtacgttcac tcacggtatc tcgatcgctt ccccgtcact
      781 ccgtctccgg gacctcgggg atctcaggcg gctcaggagg atctggagga tcgatcggct
      841 ctgctggttc agtcgaatcg cccgaaccgg tcaccggaac gggattcgag gttgaatcgc
      901 caataccctt cagggcttct ttcatggcat catcgccggc tttcaagcca tcggcgagcg
      961 ctttgtcggc ttccttcatc gccgcgtcaa cgtctttctg ggcctgatcc atggcctggt
     1021 tgaccagctg attggtcatc tgggtggcct gttgcatggc gctctggatc atggtattca
     1081 cctgcgtcat cgtctggctg accgccttct gcaccgtctg tctggtctgc tcgttcacgg
     1141 actgcaacac cttttccagg ttgttgttga cagtctgcag tgtgtttttg gtcatctcct
     1201 gcgtcatgtt ctgaagattc tttcccaggt tttcaatctg gccgtttaca ttcttcagaa
     1261 tctcgagccc ggccggattc gatgccaacg ccgtgttttt catcagatcc tggatctgca
     1321 ccccgagctg attcaccatt tccggggtga ccgccgttgt tcccgggaca accggctcag
     1381 aacctgtcgc tgcctgcgcc cacaacgcgc cagacaccga aacgatcacg accagaatcc
     1441 cgatcagaac gcttacataa cctcgcccac gtgttttcat gctcgtgccc tttccgggca
     1501 accccattga gtctcgctgc cggttcaggt atgttgtgcc cgaacgttcg agagggtgcc
     1561 cttactctct cagcatacct gtaacccggg agccaatcaa tgccgctcaa aacaatattc
     1621 cgctcacgtc ggtcctggta cctcgcgctg attctccttt cgttgagcgc ttcgtgcgcg
     1681 ctcgccggca acccggcttc ttgcgctgca gacccctggc cggcaatcgg ctccgctatt
     1741 accgcgacgt tcgcccccat caagccggga atgtcaaact tcaatctcga tttcagcctc
     1801 tggtccggct catctcttct cagcaccggt acgatcggaa tatcaaacag caagacagtc
     1861 tggttcggtc ttcgttcacc acagtcttcc tttcgttatc tcagtcaggg agcctcgatc
     1921 tcctgcctgc tcgtcactcc ctccgatacg gttctgtttc atgccaagcc cggggagtat
     1981 tccccttttc ctctggtgcg catcgacaaa gaagcgtcag gtggttttcg actcgatctc
     2041 acggtcaact accgcacctt caacggctct gagccggcag agttttccgt gcatccggag
     2101 accgccgcgt atctgaccgc caagctgcga gaccggtatc cgatggttcg tgaatccgcg
     2161 gaagagtacc gattgcgcgc ttcagagaac gcttcagaca gcgttattct cgaaaaggga
     2221 aaagacggca taggtggttt tgtcgcccac tttccgatgg agggccaacc gccactgaaa
     2281 ctgcaggtca gtccgttgaa tgtcaccggg ccgttcccga ccccaccgga taactggatt
     2341 ccggccggcg gtcgcactcc ttcatctggg ctcgacgcca ccgtgacctt ctgtaagggg
     2401 ttttactcga tcctctcaga aattctgcag cccgcgacca cgaagtaaac atccagaatc
     2461 agagcagaaa cccccgcggc atcaaacctg ataccgcggg ggttttggtt tcgatcagcg
     2521 ggcccgcttg actatgatcc actgatcaga agtctgagac ctgccttcaa tcaacttctt
     2581 gtattcgaca tactcggacg catcaagatc ggtcacgtgt cgttcgaggg tgtcggtgaa
     2641 caacacccga ttgaccgaga atccgaatct ggaatgatac atcatgccgc gtgccgaagc
     2701 gtatccgccc tcgggaagag catacacggt atagccatca ggcaaccgca gagcgatcgt
     2761 ctgttcgtcg cgacgcagga cgtcccaggt cagcggaaac cgccgcgtcg ccggccctac
     2821 cgccgccgcc gaatacgcct tcctgttgac cggcagggga atcaccagca gatcgtcgcc
     2881 actgcgcagg gcatacgcag ggacttccca ctcccaggtt aacagcggcg tgctcgcggt
     2941 atcgtcgagg ttcgccagtg taaagctcag gagccgagcc cccggaatca tttcgtgcag
     3001 catgctctcg agatacttct cctgttccag cttcgtatag tcacgcattc cacgccaatc
     3061 gcccgcggtt ccgcctctcg gctgcaaggt cgtcttcatt ttcaacgttc cgtctgccgt
     3121 gatctctccc tcatgaatac tgcggtttcc ctcttccttc gccggtacat acggcaccgt
     3181 ggctacgctg ccatcagaca agcggcgggc gctggcaccc tgcagaaagg aagggggcac
     3241 ttcaggaccg aggtcgcgca cgaccggcaa ccccatcgga aaactggcag cctgagaatc
     3301 gatctcgacc accagaccgc tcatctgcat cagactgggc acctcagacg ccacctctcc
     3361 gagcatgcgc cagcgaaccg gcaacacctg attcggcacc ccggctttca gcagcagaca
     3421 gtgatagaga aacgcccgat cgacctgatt ggcctgtttc gcctgcagcg tctgcgcaac
     3481 tgggtgcggc tgccaggagt accagtcagg aaagacatcg acatcgccga tatcgcgcgc
     3541 catgaaggta aacaagcggc gtgccttttc cgacagatca ccggttccgg aacagacacg
     3601 agtgacgatc gtctcgagcg ctgggtcggc cagcgcctgc gtgcgcagcg gggtaagaca
     3661 ggcttcgtat tcgctcgcca cccccttcca ggtgttcttc aaaccgatga ccactcgtgg
     3721 cgccagtcgc tccagttcgg gcatcagcgg ctcgtcaatc aagccttcgg tattgcaggc
     3781 ctcgaaccgc cattcctgca tctggtcttt ctcgatcgtt tcaaccttga tcaccttctg
     3841 cggatcaata gcctgaaaga ccaggtcgag tgaacgcggc gcgcgcacga tcaggcgcat
     3901 gagacgcgcc ggctcgcggc cgcgaaacag tttctcggcg agaaacggct tgagcggatc
     3961 agccttgaac cgacgaattc gatactgtcg atcgacgatc gaccccatgg taacttcagg
     4021 aatagcgaac ttgacggtat gacgtcgctg gtacaaaggc acggtgccga agtcggcctc
     4081 gtcagcaatg gtgtgatccg acacgtacct gacgcttccc ggcttgcccc cgaccaggcc
     4141 catcagaccg gattccggag ccatgatcga tcgcccgaag agcacctcgg ctttctcgag
     4201 gtcaggaaag tagccgaacg agtgattcgc atcgtcgcga gccacttcct tgaaggggac
     4261 aaacacctgt cgcgctgtat actcccaggt tcccgcatct ttcatctcgt acacttcttc
     4321 gtccagcacg tcgaggcggc tcgcctgggg gaaggcctcg gctttcggca catctttcat
     4381 cagttcccgg aggagcgggt cggagattcg ctcgaccgta tcctcccccg ccggcatctt
     4441 cagcgtttca aactcgagct tcaacacctg cgatagagca tacgatgcaa cggtaccatt
     4501 tctttcgaac tggattcccg cagcatcgag gtttttcacg gttccgaaca gttcgtcacc
     4561 gttgttcagc gtcagaatgt cggccgatag ccggtcgacc accccgccaa acgccaccag
     4621 gagacagaga gcaagcaaca gacgcaatga catcagccgg ttcatggttt tcctccctgc
     4681 gtggctttca tcagaaagat acgatcgcgg gcgcacttct gcactcgttc aatctggcgt
     4741 ttgaactccg ggtagtcctt caactcgacc cgcttggcca gccgacgaaa tgcaaagcga
     4801 taggtgatgg ccctgtctcc ttcgcgctga tactcgccga taaaggtgaa ccacggagtg
     4861 tcgacgcgta ccggttccgg aagactgcga atggttaacc cttcgccgag agaaacggtt
     4921 ccttcgtctt tctgcatctc cgtcgtgtcg tactcgatcg gatacacgcg agtcgccagc
     4981 ccggcttcgg gaaacgtcag atagtcgagc aacccgggaa cggcgaaaac catgagatcc
     5041 gcaacctcgc gcggatactg gggaatgcga tactggaagg agtaggaaaa tggaaactcg
     5101 atatcctgca gattgacgag ggtatacgtc gccaactcag caccttttcc gtatttgttg
     5161 atccgttggc tgagatacaa cccgcgctcc tcttccttga tgttcttcca gctcgcccgt
     5221 gcccaggcct cgtagtcacc ggcgtagcgc ttggtggtct cggcctgcag caggccgct
//
LOCUS       DAORHD010000141         5272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16229_length_5272_cov_4.621622, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000141 DAORHD010000000
VERSION     DAORHD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16229_length_5272_cov_4.621622"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PKO06_04305"
     CDS             complement(<1..551)
                     /locus_tag="PKO06_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HNV68897.1"
                     /translation="MNLLIACWLVPLLSGLISLVLGFLPAVARWSAAMGTAVGGLLGL
                     TLALRQIHRGESIVWEMAWNLPIGSFQLGLDALSSFFLIVISIVLLACAVYSAAYLKH
                     DDGHRPQGTVGLFFSLLAAGMMLVVLARDALLFLIVWEVMSVSAFFLVMYEDEHIPAR
                     HAGWTYLVATHLGAAFLLLLFIS"
     gene            complement(548..1231)
                     /locus_tag="PKO06_04310"
     CDS             complement(548..1231)
                     /locus_tag="PKO06_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV68898.1"
                     /translation="MELSVADVARLLKVNEQQVDDWIRHDRLPGYTVQDVLRFNRDEV
                     LEWAATHHVKVSLASGGESLPSGGIVQALERGGVFFQVPGDTREAVLHEVAQRLPLPP
                     GSDRELFEHMLVAREIANPTGIGDGIAIPHVNTPLIIPGGRSSISLLFLNHPVQWYAG
                     DRQPVFCLFVLISPTVSEHLSRLASLANLLQIKEFIALLQKRPAIDELLTKLRGWERP
                     EPVAGKEQP"
     gene            1300..4236
                     /locus_tag="PKO06_04315"
     CDS             1300..4236
                     /locus_tag="PKO06_04315"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV68899.1"
                     /translation="MDTDNQKAVQFVTRMSSASPLSPDRDFLTNARFCLVFLGTPLLL
                     AWVACVSLLDERRTNLLEWQEQRLERRLVYLESSSDTTEYLVRLLRRLERMVFSSPQP
                     EVQLARLRQSLNRRFPETFEFTLLDRHGLPIPTACDFPVPRAFLQRFAKAYQRFLNGD
                     IEALTSEKSFMQNFFGPFVPLGREFHGSLHYANSRLRRRYVYVSTPHPSGLLIVHLSA
                     GKNWDSMVLRDRVAQLNQHSDQFRWLVVNTTSLSASGATFGVASDTWRDCQQQMERSP
                     LKPVQAAGWLFMKRAISSQLLLVGGLRKTRTDSFDVARRTLTLGFMVLFLFSYACIQY
                     LSQSHLGLSIRIKLVLAFSYTAGIPLIIMGFLAGNYLREREQVLENRLYLNLEQQLAA
                     YDGRFLQSIRPLEERLQKEFLQIVLRTRDSLDEFTTRLPHLRSKINWDLLKLFNEEGK
                     PVFTDSRDRIAAGESKELGIFDKISGSVLRNLNQSITVAGDQALTEQLLSEEASGEIS
                     GTELEWVNSRVTGALGRMAPYDFGHTRFMLALTPLFDVQEYARYLAVLIWNRKTLEQT
                     YVRQTLLLQERRLSETSLFAYHRQREEMSFPRPYRLAHEAIALARQTHSSRQSTREHL
                     HTANGALLALGIRGQELSDYTLIALSSDREIRHELEVISWRFRTFALILLGISSTVGF
                     MLARRFITPIQHLTDGVTALQQRQFETRIPLIDQDEFGHLAQTFNTMIEGLEDLEVAR
                     VIQESLFPQGTVHGAGWEIYGTCHPATQVGGDYLDYFPLEDGRLVFVLGDVSGHGVSA
                     ALVMAMAKALIAHPATTYEPAAVLGTMQSVFLQVLKRKKMMTCCFGLLTPGENKVLIA
                     NAGQNFPFHLQGGTQAVELEMPGKPLGVRNTRGFECHELTLTWRDVLVLYTDGLVEAS
                     DRHGDSIGYERTKQALPDLIGETAQATEERIRQWHESIAAPGPQADDISLIIIQSHRL
                     PGAQTT"
     gene            4233..>5272
                     /locus_tag="PKO06_04320"
     CDS             4233..>5272
                     /locus_tag="PKO06_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68900.1"
                     /translation="MRDTIIRYLRHLPRALLFSAVFFGIPLGLLFLGGQQWYEEKAHR
                     MQRLLVQDLDNYLATWRTEHPFLTHLEYTLRKYGNLALSAPDTRTALKSFIHQMQKRF
                     PDTLHFLCLDNRGNVLPDLSEFQPNHTLIQRFYQDYTLLEANLPGPMTRHVHQYRDFL
                     GPMIGRDKEIKIYNKMLIASQREKRSYVYLSPATAAGMFLVFIDRHSKFDQICLTDLA
                     RTHSRVCSPWIVQLYDLKNPTAPANTLPPNLQVSADAMLGLLESGSQPQLFAHGYLWH
                     AMTHLGTFRLLIGVPDHSHLYLLEHLEVLKIAVGCVFSLLFLAVLVLFSQPTPISFSI
                     RGKLTLLFLYLVG"
BASE COUNT         1174 a         1584 c         1341 g         1173 t
ORIGIN      
        1 aaggaaatga acagaagcag gagaaacgcc gccccgagat gcgtcgcgac cagataggtc
       61 caaccagcat gtctcgccgg gatgtgttcg tcttcataca taaccaggaa aaaggcggac
      121 accgacatga cttcccagac gatcaggaac agaagagcat cacgcgccag tacgaccagc
      181 atcatccccg cggcaagcag actgaaaaac aaaccgacgg ttccctgcgg ccgatgccca
      241 tcgtcatgtt tgagatacgc cgcgctgtaa acggcgcagg ccagcagaac gatggagatg
      301 acgatcaaaa agaacgacga caaggcatcg agtccgagtt gaaaagagcc aatcggaaga
      361 ttccatgcca tttcccagac tattgattca ccacgatgta tctgccgcag tgccagggtc
      421 aatccgagca aaccaccaac agccgttccc attgccgcac tccagcgagc cacggccggc
      481 agaaaaccca gcaccagcga aatcagtccg ctgaggagcg gaaccagcca gcaggcaatc
      541 agcagattca tggctgctcc tttcccgcga ccggctctgg tcgttcccac cctcgcagtt
      601 tggtcagcaa ctcatcgatc gcgggtcgtt tctgtaacag cgcgatgaat tccttgatct
      661 gcagaagatt cgcgaggctg gccagccgcg acagatgttc actgaccgtc ggactgatca
      721 gaacgaaaag acagaacacc ggttgccggt cacccgcata ccactgcacc gggtgattca
      781 gaaacaacag ggatatggat gaccgcccac cgggaatgat cagaggggta tttacgtgag
      841 ggatggcaat tccatcaccg attccggtgg ggttggcgat ctcacgggcc acgagcatgt
      901 gttcaaagag ttcgcggtcg ctccccggcg gcaacggcag tcgttgtgcg acttcatgca
      961 gcaccgcttc acgcgtatca ccgggcacct gaaaaaaaac gccgccacgt tcaagcgcct
     1021 gaacaatgcc gcccgaaggc aacgactcgc ccccggaagc cagactgacc ttgacatgat
     1081 gtgtggccgc ccattccaga acttcatcgc gattgaatcg caagacatcc tgaaccgtgt
     1141 atccaggtaa ccgatcgtga cggatccaat catccacctg ttgttcgttc accttcaaaa
     1201 gccgcgctac atcagcgaca gaaagttcca ttgccacgcc ccgatcgaga aaagtggcgc
     1261 gattatactc ctgatgtccc ggctctgaca aggaggaact tggacacgga caaccaaaaa
     1321 gcggtacaat tcgtcacacg catgtcttcg gcttctcctc tatcgccaga tcgtgacttt
     1381 ctgacaaacg cgcggttctg cctggttttt cttggcacac cgctgctgct ggcgtgggtt
     1441 gcctgcgtct ccctcctcga tgaacgacga accaatctgc tggaatggca ggagcaacgt
     1501 ctcgagcgcc gactcgtcta tctcgagtca agttctgaca caactgagta tctcgtgcgc
     1561 ctgcttcgcc gtctcgaacg aatggttttc tcatctcctc aaccggaagt acaactcgct
     1621 cgcttgcggc agagcctcaa ccggcgcttt cccgaaacct ttgagttcac ccttctcgac
     1681 cggcacggcc tgcccattcc caccgcctgt gactttccgg ttccccgggc atttcttcag
     1741 cgctttgcca aagcctacca gagatttctg aacggagata tcgaggccct gacctcggag
     1801 aaatcgttca tgcagaactt tttcggcccc ttcgttcccc tggggcggga gttccatggt
     1861 tcgcttcatt acgccaactc gcgtctgcgc cgtcgatatg tctatgtcag caccccgcat
     1921 ccctctggtc tgctgatcgt acatctcagc gccggaaaga actgggacag catggtcctg
     1981 cgagatcgtg tagctcaact caaccaacat tccgatcaat tccgttggct ggtcgtcaac
     2041 acgacgagtc tgtcagcaag cggcgcaacc ttcggtgtgg cttctgatac ctggagagac
     2101 tgtcaacagc agatggaacg gtcacctctc aagccggtac aggcagccgg ttggctgttc
     2161 atgaagcgcg ccatctcttc acagttgctg cttgtggggg gcctgcgcaa gacccgcacc
     2221 gattcgttcg acgtcgcacg acgcacgctg acgttgggat ttatggttct attcttgttt
     2281 tcttacgcct gtattcaata tctctctcaa agtcatctcg gactttccat tcgaatcaaa
     2341 ctcgtgctcg ccttttcgta taccgccggc attcctctga tcatcatggg ctttctggcc
     2401 ggcaactatc tgcgcgagcg cgagcaggtt ctcgaaaatc gactgtatct gaacctggaa
     2461 caacaactcg ccgcctatga cggacgtttc ctccagtcga ttcggccact ggaagagcgg
     2521 ttacagaaag agtttctgca gatcgtgctg cgcacccgcg actcgctgga cgaattcacc
     2581 acgcgactgc cacacctgcg atccaagatc aactgggacc tgctcaagtt gttcaacgaa
     2641 gagggcaagc cggtcttcac cgattcgcgc gatcgtatcg cggctggtga aagcaaagaa
     2701 ctcgggatct tcgacaagat ttccggttca gtgctgcgca atctgaatca atccatcacg
     2761 gtcgccggtg atcaggccct gaccgagcaa ctgctttcag aagaagcatc gggggagatt
     2821 tcgggcactg aactggaatg ggtgaactca cgtgtcacgg gcgcgttggg acgcatggcc
     2881 ccgtatgact tcggacacac tcgcttcatg ctggctctga cgcctctctt cgatgtgcag
     2941 gagtatgccc gctatctggc cgttctgatc tggaatcgga aaacactcga acaaacctac
     3001 gtccgtcaga ccctgttgct ccaggagcgt cggctttctg aaacatctct ttttgcctac
     3061 catcgacagc gtgaggaaat gtcgtttcca cgcccgtacc ggttggcgca tgaagcaatc
     3121 gctcttgcac ggcagacgca ttcctctcga caaagtacca gagagcatct gcacacagcc
     3181 aacggcgcgc tgctcgctct cgggattcgc ggtcaggagc tctcggatta cacgttgatc
     3241 gccctgagtt cagatcgcga aattcgtcac gaactcgagg tcatttcctg gcgctttcgt
     3301 acgttcgccc tgatcctgct cggtatcagc tcgaccgtcg gcttcatgct cgcgcgtcgt
     3361 ttcatcactc caatacagca tctcacggat ggggtcacag cgctgcagca gcggcaattc
     3421 gaaacccgca ttccactgat cgatcaggat gagttcggtc atctggcgca gacattcaac
     3481 accatgatcg aaggactcga agatctcgaa gtggcacgag tcattcagga gagccttttc
     3541 cctcagggaa cggttcacgg cgccggttgg gagatctacg gcacctgtca tccagccacg
     3601 caggtcggag gggactatct cgactacttt cctctggaag acggtcgcct ggttttcgtg
     3661 cttggcgacg tctcgggtca tggagtctcg gcagctcttg tgatggccat ggcgaaagca
     3721 ttgatcgctc accccgcgac aacgtacgag ccggctgccg ttctcggaac gatgcaaagc
     3781 gtttttctgc aggtgctcaa acgaaagaag atgatgacct gttgcttcgg cctgctgaca
     3841 ccaggcgaga acaaagttct catcgccaat gccggccaga actttccctt ccatctgcag
     3901 gggggcaccc aggccgtcga actggaaatg cctgggaaac ccctcggtgt gcgaaataca
     3961 cggggattcg agtgccacga actcacactc acctggcgtg acgttctggt tctgtacacg
     4021 gacggtctgg tcgaagccag cgatcgccat ggcgacagca tcggctacga acgaaccaaa
     4081 caggctctgc ctgatttgat cggtgaaaca gctcaggcga ccgaagaacg cattcgacaa
     4141 tggcatgaat cgattgccgc gccgggacca caggccgacg atatcagcct gatcatcatt
     4201 cagtctcatc gtttgccggg agcgcagaca acatgagaga cacgatcatt cgatatctgc
     4261 gccatcttcc gcgtgccctt ctgttctcgg cggtattttt cggtattcct ctggggctgt
     4321 tgtttctcgg cggccagcaa tggtacgagg aaaaagcgca ccgcatgcaa cgtttactcg
     4381 tacaagacct ggacaattat ctggcgacct ggcgaacgga acatccattt ctgacccatc
     4441 tcgaatacac ccttcgcaag tatggcaatc tggcgctctc cgctcccgat acacgtacgg
     4501 cgctcaaatc attcattcat cagatgcaga aacgatttcc tgacactctg cactttctct
     4561 gcctcgacaa tcgcggcaac gttcttcccg atttgtccga gtttcagcca aatcatacgc
     4621 tgatacagag attttatcag gactatacat tgctcgaagc gaatcttccc ggacccatga
     4681 cccggcatgt acaccagtat cgtgattttc tgggaccgat gatcggtcgt gacaaggaga
     4741 tcaagatcta taacaagatg ctgatcgcct cgcaacgaga gaaacgctcg tatgtctatc
     4801 tctcgcccgc gaccgccgcc ggcatgtttc tggtcttcat cgatcgtcac tcgaagttcg
     4861 atcagatctg tctcacagat ctcgcacgaa cacacagtcg ggtctgctct ccatggatcg
     4921 ttcagctgta cgacctcaag aacccgacag cgcccgcaaa cactctgccg ccgaatctgc
     4981 aggtttcggc cgacgcgatg ctgggactgc ttgaaagcgg ctcgcaacct cagttgttcg
     5041 cacacgggta tctctggcat gccatgacgc atctaggcac ttttcgtctg ctgatcggcg
     5101 ttcccgatca ctctcacctg taccttctcg aacacctcga agtcttgaag atcgccgtcg
     5161 gctgtgtgtt ttcgttgctg tttctggcgg ttctcgtcct gttctcccaa ccaactccga
     5221 tttcgttttc cattcgcggc aaactgactc tgctgtttct ctatctggtt gg
//
LOCUS       DAORHD010000142         5253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16318_length_5253_cov_3.745671, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000142 DAORHD010000000
VERSION     DAORHD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_5253_cov_3.745671"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKO06_04325"
     CDS             <1..878
                     /locus_tag="PKO06_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV68901.1"
                     /translation="PGVRIVATEIDRSPALTDLLAAHDVIVNAAGNMRGDRLAEYVRA
                     NVQVPEDLVWHQQSLHKPPLFLHLSSVAAIGPTTPEAPPTETACCRAISLYGRTKRAG
                     ELSLLAARRATPNLPLLILRPPSLFGPGDPCFFDLFVWAVRGVALKLCTPKKQFNLLY
                     IGDLVNMLEQLVKHHLQIPSFENPLLHIGFPDQISDDQFSQALGRVCGRPVRPVFLSS
                     GFCRLLARLDGVRGRLAGQPHLFSRSKVDEMSCEYWLQNFDRFHSWLPNLVATPLESA
                     LRETYDWYRRQGWLQ"
     gene            875..1645
                     /locus_tag="PKO06_04330"
     CDS             875..1645
                     /locus_tag="PKO06_04330"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV68902.1"
                     /translation="MNLSCPTCRVPLQRQMTKLTCPLCHVSPLSAPSHYDFSPWFVQM
                     RDCERERVSLAYRFYSLAYAPLALINMLAVWKASLGKLTTHYQQTLKAATHPILDVAI
                     GDGALTRLATRSLALPHPLVGLDVSRDMLSQAESNLNHLPGFVSVRADACALPFPGQE
                     FQTVCCFGGLHVIAHPERAMAEMARVLRSGGRFAASILLIPHGRVSDYLSRRYVELGF
                     LSTRFTRTNAEALVQQSGLDLIDTHLNGRMLLVSARKP"
     gene            complement(1659..2645)
                     /locus_tag="PKO06_04335"
     CDS             complement(1659..2645)
                     /locus_tag="PKO06_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNV68903.1"
                     /translation="MLHIFLNQTANRGGAGERWRLVEQELQRRARPYTAHVLQAPGDL
                     DRLLPELWTQGARQFAAAGGDGTLQLVVEAVMRHVPGEQRGEIRLGAIGIGTSNDFHK
                     KGGVRPAAPRTDGQPVVPPHAASSLWNGVPCRLDEAAAHSHDVLELIVKKPDGTTETH
                     WCLQAVHAGTIPVTNQNLTRGGGWCQWMYGHWYWLSLQMAAMHTLFSYAGFEAEIEVN
                     SASISGTFTGMTLLKRCNIAGQFVFATHRTPNDGQIDLAACRKVSGWRMIQLIIAFTE
                     QGFAGHPEVEFRETSEVRAQFSAPQWIDYDGELVQAVEAQVRVLSAALTMLG"
     gene            complement(2648..3925)
                     /locus_tag="PKO06_04340"
     CDS             complement(2648..3925)
                     /locus_tag="PKO06_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV68904.1"
                     /translation="MAVKNLDVANFDVKQLLLDQTMSLSQKMNLFHRVIQESKRLKLY
                     LYFRTTNGPSEAHIDATTLETGEVKNMIMMASNNYLGLSTHPEVKRRTIETVEKWGIG
                     MAGPCTLNGNTVLHQAFEDHLARFKHCEAAMLFASGYAANIACITGLLGKNDIFIGDE
                     LNHASLIDGARYSGARRMVFKHSDPADLERCLKETQAETGTRLVAVDGVYSMDGEIAP
                     LRPIVDLCKKYGAMLMVDDAHGTGAVGKTGRGSLEYCGVEGEVDIVMGTFSKAFGSFG
                     GFLAGTHAFINWMRHFGRAGMFTASVAPMNVAACHAALEIMEQHPELVMRLQQNAARV
                     RRSLRDMGFEISNHETAIIPIFIRDRARAYGIVKEAYERGLFLSLIEYPAVAPGTERV
                     RLTVMATHTDADLDTAMALLKELGQKYGVLKGS"
     gene            complement(3916..4584)
                     /locus_tag="PKO06_04345"
     CDS             complement(3916..4584)
                     /locus_tag="PKO06_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68905.1"
                     /translation="MTKTATVPTPVPAGTMPPMICLTGIDGCGKSTHLRTLTERLQQH
                     LGEQIPVLSVWDISRLPRYRAHPLIADRETLYQYLGSLHGGARGLFILHALRESLDNI
                     LDAHPRLILVDGYWYKYLLTEILHDPGCEWLYDCAVAFPRPIGTVLLDLPAEASFQRK
                     PALTPYECGFARPGPDTFIPFQNRLRAALQEMARRERWPVIDADRPVFAVAEQIWTQV
                     EKWL"
     gene            complement(4590..>5253)
                     /locus_tag="PKO06_04350"
     CDS             complement(4590..>5253)
                     /locus_tag="PKO06_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68906.1"
                     /translation="RIMCDIVRAYLEHGPSRVSRVAILVAGARAYKRVLFHQGMPRND
                     DLFVGLMAAILRTSRDEMRDVKQRFLESASLRAAVARSIRPIPEALELFKRLATTDTR
                     RHVVATNPIMPARFNALRLAEAGYPIERFDYICGSEDFSGQKHLPSFYPDLLARLHAD
                     PTEAMMIGNDPKKDLAVGATGIRTFLLRTPFTVLRRDWPGRPDWEGDYGTLEKRLSEG
                     GL"
BASE COUNT         1073 a         1670 c         1430 g         1080 t
ORIGIN      
        1 tccccggtgt ccgaattgtc gctaccgaga tcgatcgatc gccggcactg accgacttgc
       61 tggcagctca tgacgtcatc gtgaacgcgg ccggtaacat gcgcggcgac agactcgccg
      121 aatatgtcag agcaaatgtc caagttcccg aggatctggt ttggcatcaa cagagccttc
      181 acaaacctcc acttttcctg catctgtcat cagtggcagc gatcggcccg accacgcctg
      241 aagctccacc aactgaaact gcctgctgcc gcgccatcag tctgtatggc cgaacaaagc
      301 gcgccgggga attgtctctc ctggcggccc gccgcgccac cccgaacctg cccctgctca
      361 ttctccgccc accgtcgctg ttcggtccgg gtgacccctg tttcttcgat ctgtttgtct
      421 gggcagtcag aggcgtagcc ctgaaactct gcaccccgaa gaagcagttc aacctgctct
      481 acatcggcga cctggtcaac atgctcgaac agctggtcaa gcatcacctc cagattcctt
      541 ccttcgaaaa tccgctgcta cacatcggtt ttccagacca gatttcggac gatcagtttt
      601 cgcaggcgct cggtcgtgtc tgtggtcgac cggtacgtcc ggtgtttctg tcgagcggat
      661 tctgccgact gttggcgcgg ctcgacggag tgcgcggtcg tctggccggg caaccccatc
      721 tgttcagcag atcgaaggtc gatgagatgt cctgcgaata ctggctgcaa aactttgacc
      781 gttttcactc atggcttccg aacctggtcg ccacaccact cgagtccgcg ctgcgtgaga
      841 catacgactg gtaccgcagg cagggctggc tgcaatgaat ctctcctgcc cgacctgtcg
      901 agttccgttg cagcggcaga tgaccaagct gacctgcccc ctatgccacg tttcgcctct
      961 ctctgcgcca agccactacg atttttcgcc ctggtttgtt cagatgcgcg attgcgagcg
     1021 cgaacgcgtc agcctggcct atcgcttcta ctcattggct tatgctccac tggctctgat
     1081 caacatgctt gccgtctgga aagcctcact ggggaaactg acgacgcact accagcagac
     1141 tctgaaagca gctacccatc cgatcctcga cgttgcgatc ggcgatggtg cgttaacacg
     1201 gctggccaca cgcagcctgg ccctgccgca tccgctcgtg gggctcgatg tatcacgaga
     1261 catgttgagt caggcagaga gcaatctgaa ccatctcccc ggattcgtca gcgtacgagc
     1321 tgacgcctgt gctctgcctt ttcccgggca ggaattccag accgtctgct gtttcggcgg
     1381 gctgcatgtc attgcccacc ccgaacgtgc catggccgag atggcccgtg tcctgcgctc
     1441 cggtggccgc ttcgccgcct caatcctgct gattccgcac gggcgggttt ccgattatct
     1501 gtcgcgccgg tatgtcgagc tcggatttct ctcgacccgc ttcacccgga ccaatgccga
     1561 agccctcgtt caacagagtg gcctcgacct gatcgacacg cacctgaacg gacgcatgct
     1621 tctcgtatca gcccgtaaac cctgaaacgt gcggagtttc agccgagcat ggtcagggct
     1681 gccgaaagca cacgcacctg cgcctcgacc gcttgtacca gttcaccatc gtaatcgatc
     1741 cattgtggag cagaaaattg agccctgact tctgacgttt cacggaactc gacttcagga
     1801 tgaccggcaa aaccctgttc agtgaaggca atgatcagct gaatcatgcg ccagcccgat
     1861 actttgcggc acgccgccag atcgatctga ccgtcgttcg gggtacgatg ggtggcgaag
     1921 acgaactgac ccgcaatatt acagcgcttg agcagagtca tccccgtgaa ggttccgctg
     1981 atcgaagcgc tgttcacctc aatctcggcc tcgaatccag catagctgaa caaggtatgc
     2041 atggccgcca tctgcagcga caaccagtac cagtgaccat acatccattg gcaccagcca
     2101 ccgcctcggg tgaggttctg attggtcact ggaatcgtgc cggcatgcac ggcctgcagg
     2161 caccagtgcg tctcggtcgt gccatcaggc ttcttcacga tcagctcaag cacatcgtga
     2221 ctgtgcgcag cggcttcatc aagacgacac ggcactccgt tccagagaga actcgccgca
     2281 tggggcggca caacaggctg tccatcagtg cgaggagcgg cggggcgcac cccacctttc
     2341 ttgtgaaagt cgttactggt accgatacca atcgcgccca acctgatctc gccacgctgc
     2401 tcaccgggaa catgacgcat gaccgcttcg accaccagct gcaacgtgcc atcaccaccg
     2461 gcggcggcga attgacgcgc gccctgcgtc cacaactcgg gcagcagacg atcgagatcg
     2521 ccgggagcct gcagcacatg cgcggtatag ggccgcgcgc gacgctgcag ctcctgctcc
     2581 accagtcgcc aacgttcacc cgctccgccg cgattcgcgg tctgattgag aaagatgtgc
     2641 agcatgctca cgatcctttc aacacgccgt atttttgtcc gagctctttg agcaacgcca
     2701 tggctgtatc gaggtccgca tcggtatgtg tcgccatcac cgtcagtcgt acccgctccg
     2761 ttccgggggc cacggcgggg tactcgatca gcgacaggaa caacccacgc tcgtaggctt
     2821 ccttgacgat accgtaggcc cgtgcccgat cacgaatgaa tatcggaatg atcgccgtct
     2881 cgtgattgct gatttcaaac cccatgtcgc gaagactgcg acgcacccgg gcggcgtttt
     2941 gctgcagccg catcaccagc tcaggatgct gttccatgat ctcgagcgcg gcgtggcagg
     3001 cagccacatt catgggagca accgacgccg tgaacatgcc cgcacggccg aagtgccgca
     3061 tccagttgat aaaggcgtga gtcccagcca ggaaaccacc aaacgagcca aaggccttgc
     3121 tgaaggtacc catgacaatg tcgacttcac cttcgacacc gcagtattcg agagatccac
     3181 gaccggtctt cccgacggcg cccgtgccat gtgcgtcatc gaccatcagc attgccccgt
     3241 atttcttgca caagtcgacg atcggccgca gcggcgcgat ttcgccatcc atgctgtaaa
     3301 caccgtcgac ggccaccaat cgagtgcccg tctctgcctg agtctctttc agacagcgtt
     3361 cgagatcggc cgggtcggaa tgcttgaaca ccatgcgccg ggctccgctg taccgtgctc
     3421 catcaatcag gctggcgtgg ttcaactcgt caccgatgaa gatgtcgttc ttgccgagca
     3481 atccggtaat acaggcaata ttcgcggcat aacccgaggc aaacagcatg gccgcctcac
     3541 agtgtttgaa acgcgcgagg tgatcttcga acgcctgatg cagcacagta ttcccgttca
     3601 gcgtgcaggg accggccatg ccgatacccc acttttcgac cgtctcgatc gtgcgccgtt
     3661 tcacttcagg atgcgtcgat agtccgaggt agttgttcga cgccatcatg atcatattct
     3721 tcacttcgcc ggtttcgagc gttgtggcat cgatgtgcgc ctcggacgga ccgttggtcg
     3781 tgcgaaagta gagatacagt ttcagtcgtt tcgattcctg aatcacccgg tgaaacaggt
     3841 tcatcttctg cgacagactc atcgtttgat caagtaacag ttgctttaca tcgaagttcg
     3901 cgacatccag attcttcaca gccatttttc cacctgtgtc cagatttgtt cagccacggc
     3961 aaacaccggg cgatcggcat cgatcaccgg ccaacgctcg cgacgggcca tttcctgcag
     4021 tgccgcccgc aagcggttct gaaaggggat gaaggtgtca ggtccggggc gcgcaaaccc
     4081 gcactcatac ggagtcaaag ccggcttgcg ctgaaagctc gcttcggcgg gcagatccaa
     4141 cagcacggtg ccgatcggcc gcgggaacgc gacggcacaa tcatacagcc attcgcaccc
     4201 cgggtcgtgc agaatctcgg tcagcagata cttgtaccag tagccatcga cgagtatcag
     4261 cctgggatga gcgtcaagga tgttatcgag agactcgcgc agcgcatgca gaatgaagag
     4321 cccacgcgca cccccgtgca aactccccag atactggtag agagtttcac gatcggcaat
     4381 cagagggtgc gcccgatagc gtggcagtcg agaaatgtcc cagacactga gaaccggaat
     4441 ctgctcgcca agatgctgtt gcagtcgttc agtcaacgtt ctcagatgcg tcgactttcc
     4501 gcagccatcg atccccgtca ggcagatcat cggcggcatc gttcccgccg gaaccggagt
     4561 tggcacggtc gcggttttgg tcatgagctt cacaaacctc cctccgacaa acgtttttcc
     4621 agagttccgt aatccccttc ccagtccggt cgaccgggcc aatcacgacg cagcacggtg
     4681 aacggcgtgc gcagcagaaa ggtgcgaata cccgttgccc ccaccgcgag atctttcttc
     4741 gggtcattcc cgatcatcat cgcctcggtt ggatccgcgt gcaggcgtgc cagcaggtca
     4801 gggtaaaacg acggcaggtg tttctgaccg gaaaaatcct cgctgccgca gatataatca
     4861 aaccgttcga tcgggtatcc ggcctcggca aggcgcagcg cattgaagcg cgccggcatg
     4921 atcggattgg tcgccacgac gtgccgacgg gtgtcggttg tcgccaagcg cttgaacaac
     4981 tccagcgctt cgggaatcgg gcgaatcgac cgcgccacgg cggcacgcaa ggatgccgac
     5041 tcgagaaatc gctgcttcac gtcgcgcatc tcgtctcgtg acgtgcgcag gatcgccgcc
     5101 atcaggccga cgaacagatc atcgtttcgc ggcatgccct gatgaaacag cactcgtttg
     5161 tatgccctcg caccggcaac caggatggct actcgagaga cacgactggg accatgttcc
     5221 agataggcgc gaacgatgtc acacatgatg cgt
//
LOCUS       DAORHD010000143         5243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16360_length_5243_cov_4.300694, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000143 DAORHD010000000
VERSION     DAORHD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16360_length_5243_cov_4.300694"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKO06_04355"
     CDS             complement(<1..999)
                     /locus_tag="PKO06_04355"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68907.1"
                     /translation="MQTRLMTIMFIDVQGFTKRTASQTLEETKVFIEETRTFVQEHLE
                     KWNGKLIKTIGDGFLGSFEAPTSAIQAALEIQRKLEARNANILNPENFVRFRIGINTG
                     EIAVDESGDVFGDPVNIAARIESFCEPNEVFISEATYLAINRNQFQLKDLGPQMFKNA
                     TREIRIYKILRHGPELGGASTLTAPKPSMKETVSGGGAHASESKTRLPANWAKIVGAI
                     VAFLLIAALFCVALKKVLQRGKGSGSTATETVALPPLVPLPGLKNGQAPVLVPVPSLA
                     SVSAEPKPTAGPATMTSTDEDDEDLSGDEDLLADNPPGEKPMSDKRPRKPGFFKLFA"
     gene            1141..2865
                     /locus_tag="PKO06_04360"
     CDS             1141..2865
                     /locus_tag="PKO06_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV68908.1"
                     /translation="MLILGINSAYHETAAALLDDGRLIAFVEEERINRRKHGKPSAVD
                     NPDELPLGAIRECLSMAGATFDDVDLIGYSFDQKGRKANIGVDTFATSGSWGTQEGEE
                     RFHAALMRVPDNLQKLGTKPLAPRFRWIPHHLAHAGSAFLASPFERAVVMTIDGIGEF
                     DSLCVWSGEGTQLKPLFVLPYPHSLGFLWEKVCQFLGFSEYDACKVMGLSSYGNRNRL
                     AAELAQIAQLHEDGTFRLSLEHLHFRAATFDGLSEIFGPPRLPGEPILERHQDLAAAV
                     QAFTEEALMAMATRARRDTRAPHLTLSGGVALNCVANSRLEREAGYERLFVQPAANDA
                     GTALGAALVLWTQECGKERVFVQDHPYYGPSYTVADCERAISERGLTADRPTRPEEAV
                     ASLVADGRVVGWFQGRMEMGPRALGNRTLLADPRRHDMRDLLNRKVKHREPFRPFAPS
                     VLAEKATDWFDMPGRTDSTDFMLFAYPARPDKRERIPAVVHVDGTSRIQTVRASSAPR
                     YHSLISAFERRTGVPMVLNTSFNDSEPIVMSPTHAINTFLKTGIDAVMLEGLLVRKPG
                     VEPPVTEP"
     gene            2869..3837
                     /locus_tag="PKO06_04365"
     CDS             2869..3837
                     /locus_tag="PKO06_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68909.1"
                     /translation="MSDSLSTEHDFAIPRDGSLFNPIDGPRGRRLALVFGNEAPGGRC
                     PFHTAQCSHCDLGAGEGIRFTTEINVRRLTFFTRHYAEIFPMIEHLVIYNSGSTLNPL
                     ELSLDTLQHILAFAASLPKCRRVSFDTREDFVTPERVARLADGLRSDQQFGLTLGVES
                     QNDHVRMQLLGKSMSREEVAAVFQHLSRCNPRSRVEMNVVFQPPGLTGQAAVDDALAT
                     IRFGLELMDQSGVPVDFNFHPYYPSWKGTREFPNHPRANLQDTLRALILIHREIRTHG
                     GDSAIFVGWNDEGHDLQPSSKSREINLYQPGIVAFNRSQDEADLHL"
     gene            complement(3919..4440)
                     /locus_tag="PKO06_04370"
     CDS             complement(3919..4440)
                     /locus_tag="PKO06_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNV68910.1"
                     /translation="MKQKVIEVLNKARGMELHAIHQYMNQHYNLDNLDYGELAAKMKL
                     IAIDEMRHAEAFAERIKEIGGEPTTDPAAKTTKAQKVEAIYPFDSELEDDTLLQYNQF
                     LNICRENGDSISAKVFETIIAEEQLHFNYFDNVNEHLKKLGAAYLARIAGTPAEVGAV
                     SPGFVGQGADPAA"
     gene            complement(4591..>5243)
                     /locus_tag="PKO06_04375"
     CDS             complement(4591..>5243)
                     /locus_tag="PKO06_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015774789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV68911.1"
                     /translation="CAANPACGRCRTVCPTGAILGEGKDLSIDLGRCVFCGECERVCP
                     SRAIVFTNDHRLAVRKREHLIYRGGPLPEFLPLDEPVRSLLSHSFKIRQVSASGCNAC
                     EADINVLGTLAFDWGRFGISVVASPRHADALLVTGPVAQQMQLALKKTFDATPTPRVV
                     VAVGSCAISGGPFIDHPEVTNGALTTLAVDLFIPGCPPHPMTILDGVLRLLGRKTA"
BASE COUNT         1081 a         1584 c         1441 g         1137 t
ORIGIN      
        1 ggcaaacagt ttgaaaaagc caggttttct cggccttttg tcgctcatcg gcttctctcc
       61 cggtggattg tccgccagca gatcctcgtc acccgacaag tcttcgtcat cctcgtcagt
      121 cgaggtcatc gttgctggtc ctgccgtggg cttgggttcg gcggaaacgc tcgccagtga
      181 tggcaccggt accagcacag gagcttggcc atttttcaga ccgggcaacg gcaccagcgg
      241 aggtagggcg acggtttccg tggcagtcga cccagaacct ttgccgcgtt gcagcacttt
      301 cttcagagcc acgcagaaaa gggcggcaat caacagaaag gccacaattg cccccacgat
      361 cttggcccag ttcgccggca gacgagtctt ggattcagaa gcatgcgctc caccaccaga
      421 taccgtttcc ttcatcgatg gtttcggcgc ggtcagagtg ctggccccgc ccaactccgg
      481 cccatggcgg agaatcttgt agatacgaat ctcgcgcgtt gcgttcttga acatctgtgg
      541 gccaagatct ttgagctgaa actgatttcg attgatcgcc agataggtgg cttcggagat
      601 gaacacctcg ttcggttcac agaaactctc aatgcgtgcg gcaatgttca ccgggtctcc
      661 gaacacgtca ccgctctcgt caaccgcgat ttctccagtg ttgatgccga tccgaaaccg
      721 aacaaagttc tcgggattga gaatgttggc gttgcgcgcc tcgagcttgc gctgtatttc
      781 cagagccgcc tgaatagcgc tggtcggcgc ttcaaaggac ccgagaaacc catctccgat
      841 cgtcttgatc agcttgccgt tccacttttc caggtgttcc tggacaaagg tgcgggtttc
      901 ctcgatgaag accttcgtct cttcgagagt ctgactggct gtacgtttgg taaagccctg
      961 cacgtcaatg aacatgatgg tcatcaatcg ggtctgcaca caactacctc ccggagacgg
     1021 tacgcgcatt ccctttatac ccgaagagtc ttctgccgtc tacgccgtca acgctgggca
     1081 ttgcccggga aaggtgatac actcgtgcca caccgctttc caccacaggg gaggacacgc
     1141 atgctgatac tcggcatcaa ctcggcttat catgagactg ctgctgccct gctcgacgat
     1201 gggcgtctga tcgcttttgt ggaagaggag cgcatcaatc gccgcaagca tggaaaacct
     1261 tcagccgtcg acaaccctga tgaactgccg ctcggtgcta ttcgcgagtg cctctccatg
     1321 gctggagcca cgtttgatga tgtcgatctg attggctatt ccttcgatca gaaagggcgc
     1381 aaggccaaca ttggcgtcga taccttcgcc acaagcggca gctggggaac tcaagaaggg
     1441 gaagaacgct ttcacgctgc gttgatgcgc gttcccgata acctgcaaaa gctcgggacc
     1501 aaaccgcttg cgccaagatt ccgctggatt cctcaccatc tggctcatgc aggaagcgcg
     1561 tttctggctt ccccctttga gcgtgccgtg gtcatgacca ttgacgggat cggcgagttc
     1621 gactccctgt gtgtctggtc gggcgaaggt acgcagctga agcctctctt tgtcttgccc
     1681 tacccgcaca gtctcggttt tctctgggaa aaggtctgtc agtttctcgg cttctctgag
     1741 tatgatgcct gcaaggtcat gggcttgagc agttacggca accggaatcg tctggccgca
     1801 gagctggctc agatcgcgca gttgcacgaa gatggcacgt ttcggctcag tctcgagcat
     1861 ctgcatttcc gagccgccac attcgatggg ctctcagaga ttttcggacc accacgcctg
     1921 ccgggcgaac ccattcttga acggcatcag gatctcgccg ccgccgtgca ggcctttacc
     1981 gaagaagcgt tgatggccat ggccacgcga gcccgacgcg atacccgggc cccccacctg
     2041 actctttcag ggggagtcgc tctgaactgt gtcgccaata gccggctcga acgcgaagcc
     2101 ggctatgagc gcctgttcgt tcaaccggcc gccaacgatg ccggcaccgc ccttggtgcg
     2161 gcactggttc tctggaccca ggaatgcggc aaggagcggg tattcgttca agatcacccg
     2221 tactatgggc ccagttatac cgtagccgac tgtgaacgag ccatcagtga acgtggtctc
     2281 accgctgacc gcccgaccag accagaagaa gccgtcgcct cactggtggc ggacggacga
     2341 gtcgtcggct ggtttcaggg gcgtatggaa atgggtccaa gagccctggg aaaccgcact
     2401 ctgcttgctg atccccgacg tcacgacatg cgtgatctgt tgaatcgcaa ggtcaagcat
     2461 cgtgagccat tccggccttt cgcgccaagc gtgctcgcgg agaaggcgac agactggttc
     2521 gacatgccgg ggcgcaccga ctcaacggat ttcatgcttt tcgcctatcc cgcgcgtccc
     2581 gacaaacgcg aacgtattcc cgccgtcgtg cacgtcgacg gtacaagtcg aattcaaact
     2641 gtacgagctt cctcagctcc ccgataccat tccttaatct cggcattcga acggcgaacc
     2701 ggcgtgccga tggttctcaa cacgtcgttc aacgattccg aaccgatcgt catgtctccg
     2761 acgcacgcca tcaacacatt cctcaaaacc ggaattgatg cggtcatgct tgaaggtctt
     2821 ctcgtacgca agccaggcgt cgaaccgccg gtcacggaac cctgaatcat gtccgattcg
     2881 ttgtccaccg aacacgattt tgccattcca cgggacggct cactcttcaa ccccatagat
     2941 ggaccgcgcg ggcgacggct ggcactggtc ttcgggaacg aagcaccggg cggtcgctgt
     3001 ccgtttcata ccgctcaatg ctcgcactgc gatctcggcg cgggcgaagg catcagattc
     3061 acgaccgaga tcaacgtacg ccgtctgaca ttcttcaccc ggcattacgc tgagatcttt
     3121 ccgatgatcg aacatctggt catctataac tcaggctcga cgctgaatcc gctcgagctt
     3181 tcgctagaca cactgcagca cattcttgcg ttcgcggctt cgctgccgaa gtgccgccgg
     3241 gtcagttttg acacgcgcga ggatttcgtg acccccgagc gggttgcccg gcttgccgat
     3301 ggcctgcgct ctgaccaaca gttcgggctg acgctcggcg tcgagagcca gaacgaccat
     3361 gtgcgcatgc aactgctcgg aaagagcatg agtcgtgagg aagtcgccgc ggtgtttcag
     3421 catctgagcc gttgcaatcc gcgcagtcgc gttgaaatga acgtggtgtt tcaaccaccg
     3481 ggtctgaccg gacaggcggc ggttgacgat gcgctggcta ccattcgatt cgggcttgaa
     3541 ctcatggatc aatccggagt gcctgtggat ttcaattttc atccctatta cccgagctgg
     3601 aaaggaacgc gcgagtttcc caaccatccc cgagccaacc tgcaagacac gcttcgggca
     3661 ctgattttga ttcatcgcga gattcgaacc catggcggcg acagcgccat cttcgtcggc
     3721 tggaacgacg aaggccacga cctgcaaccc tcgtcgaagt cacgtgaaat caacttgtac
     3781 caaccaggaa tcgtggcctt caatcgctct caggacgagg ctgatctcca tctctgaaca
     3841 gaagcatccg actctctcca gagacacgaa cggcgactcc tggagtcgcc gttcgtgtct
     3901 cagagcctat cgattccttc aggccgcggg atcagctccc tgaccgacga atccggggct
     3961 gaccgcgccg acttccgccg gcgttccggc gatacgggcg agatacgcgg cacccagctt
     4021 cttgaggtgc tcgttcacgt tgtcgaagta attgaagtgc aactgctctt cggcgatgat
     4081 ggtttcgaac actttggcgc tgatactgtc gccattctcg cggcagatgt tgagaaactg
     4141 gttgtactgc agcagcgtat cgtcttcgag ctcagagtcg aacggataaa tcgcttcgac
     4201 cttctgggcc ttcgtggtct tcgcggccgg gtcggtcgtc ggctctccgc cgatctcttt
     4261 aatacgttcg gcgaaagctt cggcatgccg catctcatca atggcaatca gcttcatctt
     4321 cgcggccagc tcaccgtagt ccagattgtc caggttataa tgctgattca tatactgatg
     4381 aatcgcgtgc agttccatgc cacgtgcctt gttgagaacc tcgatcacct tctgtttcat
     4441 cacttcacga gacgtttttg ccacgactct tcctcctgga atgcaaattg ttgatgtctg
     4501 cagcatacca catcccgcgt gttcaaaaaa cggcgcttgc cggcgattcg tctctcaatc
     4561 cgaatcattc cgatttacga acgtgacgga tcaggcggtt ttacgaccaa gcagacgcag
     4621 cacgccgtcg aggatcgtca tcggatgcgg cgggcaaccc ggaatgaaca gatcaaccgc
     4681 aagcgtcgtc aacgctccgt tggtcacttc cgggtgatcg atgaacggac cgcctgaaat
     4741 cgcacaggag ccaaccgcca ccacaacgcg aggtgtcggc gtagcatcga aggtcttctt
     4801 cagggccaac tgcatctgct gggccaccgg accggtcacc agaagagcat cggcatgccg
     4861 cggtgaagcg acgactgaga tgccgaacct tccccagtcg aacgccagag tacccaacac
     4921 gttaatgtcc gcctcgcagg cattacaccc ggaagcgctg acctggcgga tcttgaagga
     4981 gtgcgacaga agcgagcgta ccggctcatc gagcggaaga aattccggca gtgggcctcc
     5041 gcgatagata agatgttcac gtttgcggac ggcgaggcga tgatcattcg tgaagacaat
     5101 cgcccggctt gggcacaccc gctcacattc cccgcagaat acgcagcggc caagatcgat
     5161 cgacaggtct tttccttcac cgagaatagc tccggtcggg caaacggtgc gacagcgacc
     5221 gcaggcggga ttcgccgcac agg
//
LOCUS       DAORHD010000144         5232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16423_length_5232_cov_3.660421, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000144 DAORHD010000000
VERSION     DAORHD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16423_length_5232_cov_3.660421"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKO06_04380"
     CDS             <1..217
                     /locus_tag="PKO06_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68912.1"
                     /translation="SAQRQKATLVCTEKDLVKIPVSWATSHSLHVFSIKLLPDEQTTF
                     LEVFRRQGLSLPASTSATAFEGHVSRR"
     gene            complement(221..472)
                     /locus_tag="PKO06_04385"
     CDS             complement(221..472)
                     /locus_tag="PKO06_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68913.1"
                     /translation="MGAGFLGFGGLAILATGSVWLLLALSFIGWKPLWEFAYELLRQV
                     CLFIYGFFCVIDFICIFGSLGMMLLDLLSGIGWIFMLFL"
     gene            1028..3568
                     /locus_tag="PKO06_04390"
     CDS             1028..3568
                     /locus_tag="PKO06_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HNV68914.1"
                     /translation="MIDLIYNLSLLVALSVISGFISQRWAKNTWHGEILQGTLFGCTA
                     MLGMVRPLVLAPGLIFDGRSVMISLCGLFFGLQATLIAVLLTGFLRLWQGGIGATVGV
                     MVILSSALIGLLSAHLRKASLADTRALDLYFFGLAVHVAMLLIMLLLPFEMAMQTLRS
                     IGLPVMLTYPLATVLIGKILSDQERNTQTINALRTSEARYRFLADNTSDVLWIFNFDT
                     QAWDYISPSVSRLRGYSAEEVQRQTLAQVLSPQSLSSISNTLAERAALLQHHPERPHT
                     YTDEVEQTCKDGTTVCAEIASRFTYNDRHERIVIGVSRNIQERKLAEVRMKASQEALQ
                     SSEDRYKALLQSVPDLLFLLDRQGVFRDYHANDPAKLMVKPEQFLNRPVNEILPPEIA
                     AQTMQLLATLFETGSHQQYEYALHLPDGKRFFENRLFPCGKDLALSIIRDITDLKTSE
                     EEKLQLLQQVHQSQKLESLGVLAGGIAHDFNNILMGILGNVELAEVDVKPGSPVLSRL
                     GKITAAAQRAAELCRQMLTYSGSSSLSCESIVLPLLVPEIIRLLKSNIPAKTAVRLDF
                     STPLPPIFADPYQIRQMLRHLIINASESITHDHGLIMVSAVSRRYTSDDLAATELAPT
                     LPAGEYIAVSVEDNGCGMSAETRRRMFEPFYSTKFVGRGLGLASVLGIVRAHSGAIII
                     DSQPEKGTRIQVLLPTAKGLQNSDVPSTPPASSPRPTILFADDEESLRDIAQVTLQRL
                     GYQVLLARTGQEAIDLYKTHRQEVRVTILDLSMPGMDGIQTLDELRHLDPNALVIIAS
                     GYSEEDIRDRFVSQAPSAVLQKPFTMSQLKTVFARLNLQVLFQPSVRT"
     gene            complement(3554..4183)
                     /locus_tag="PKO06_04395"
     CDS             complement(3554..4183)
                     /locus_tag="PKO06_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV68915.1"
                     /translation="MNFDQQLFHLLNGGLTHPWLDYAMPIITDQKNWLPFLAAIWFWL
                     LLGRNGAHRRLAVLLVLIVIGADQMSSAVIKPLIRRARPCCAEPNARLLIPCKTSKSF
                     PSSHAANTAGVAGVVWLEAGWRAGLPVWVIAVMVGYSRIYVGVHYPADVLCGHLLGLL
                     LGACLIFLANRLWAKKINSPLSQDKTIVATDSHSQHTGTDQSSDSSGSD"
     gene            complement(4205..>5232)
                     /gene="dnaB"
                     /locus_tag="PKO06_04400"
     CDS             complement(4205..>5232)
                     /gene="dnaB"
                     /locus_tag="PKO06_04400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV68916.1"
                     /translation="LRRLISVTGEVARDAYEPQAQAELLLDEAEQKVLQLSQHRATRP
                     YIAIKDLLKDTYEELEMICERKQPITGVPTGFKGLDEYTSGFQPSDLIIVAARPSMGK
                     TAFCLNIAVNAAQLHPERRGVLIFSLEMSRSQLMRRILCSEARIDGQKIRSGYLAKED
                     WTKLAMTSGMLDVLPVFLDDTPNAPLLEIRSKARRCWAQEKIGMIVIDYLQLITYSMS
                     GSGGENRQQEISAISRSLKGLARELQVPIICLSQLSRGVETRTDKRPILSDLRESGAI
                     EQDADVVLFLYRDSYYNPKKEESRNKAEVIIAKQRNGPVGSFELGFFSEFARFDNLDA
                     THTGGYD"
BASE COUNT         1191 a         1563 c         1343 g         1135 t
ORIGIN      
        1 ctctgcgcag cgccagaagg caactcttgt ctgcaccgag aaagatctgg tgaagattcc
       61 ggtttcctgg gcaacaagcc actcccttca tgtcttcagc atcaaactcc tgccggacga
      121 gcagaccacg tttctcgaag tatttcgtcg tcaggggctc agccttcctg cgtcaacctc
      181 tgcaacagcg tttgaaggtc acgtcagcag gcgttaaagc tcagagaaag agcatgaaga
      241 tccagccgat ccctgagagc agatccagca gcatcatccc caggctgccg aagatgcaga
      301 tgaaatcgat aacgcagaaa aaaccgtaga tgaagaggca cacctgacgc agcagctcat
      361 aggcgaactc ccacagcggc ttccagccga tgaaactgag cgccagaagc agccacacac
      421 tgccggtagc caaaattgcc aacccaccaa accccagaaa acctgcgccc atggcttcct
      481 ccttgtcact ctctcacccg caggttttgt gccagggaac gcacaacggc gggatgcgag
      541 taatcacggg gaatgcgtca tggccgtacg tctggctatg acacttctga cacactccgg
      601 aatcaagatg catgccatca gcacactgcc tgaatctgtt tgattctgtc ccgttttcct
      661 cttggcgtga gcgaaacttg tgagccgtcc ggcgagacgc ggacggatcc caaaccgggc
      721 tgccggatcg caggcggcga ttcggccgtc tgtcttcaca gggatgcgaa gtcgataagg
      781 tttcagcctg cgtttcgaga tgaagggcgc actccctgcc gcgacggcaa atcgtggagc
      841 gatgccaaga gaataaccca agccccagaa tcaaatgact ctggaagatg acattgaaag
      901 ggctcgatgg ttgccgtatg atggtctcgt gacttcctga ttgatctttt acggccactc
      961 aatttctgcg cggaaacccg ccgcatccca tcaggctctg cccctcttct cgagaaccac
     1021 tgccaccatg attgatctga tctacaacct gtctcttctc gtcgctctca gcgttatctc
     1081 cggtttcatt tcacaacgct gggcgaaaaa tacgtggcat ggcgaaattc ttcagggaac
     1141 acttttcgga tgcaccgcca tgctcggcat ggttcgcccg ctggtgcttg cccccggtct
     1201 gatctttgac ggccgttccg tcatgatcag cctctgcgga ctattcttcg gtctgcaggc
     1261 gacactcatc gccgtccttc ttacaggctt tctgcgtctc tggcagggag gcatcggcgc
     1321 gactgttgga gtgatggtca ttctgtcttc ggcactcatc ggcctgctgt ccgcccatct
     1381 gcgtaaggct tcgctggcag acactcgagc cctcgacctg tacttttttg gtctcgccgt
     1441 tcatgtggcc atgctgttga tcatgttgct tctgcctttc gagatggcca tgcagacact
     1501 tcgcagtatc ggcctccctg tcatgctgac gtatccgctg gctacggtac tgatcgggaa
     1561 gattttgagc gaccaggaaa ggaatacgca gacgatcaat gccctgcgta ccagtgaagc
     1621 acgctacaga tttctggccg acaacacctc agatgtcctc tggatcttca atttcgacac
     1681 gcaggcctgg gactatatca gcccctcggt cagccgtttg cgtggctact cagccgaaga
     1741 ggttcaacga cagaccctgg cacaggtgct ttccccacag tctctttcga gtatcagcaa
     1801 cactctcgcc gaacgcgcgg cccttttgca gcatcacccc gaacgaccgc atacatacac
     1861 cgatgaagtc gagcagacat gcaaggatgg gacaaccgtc tgtgcggaaa ttgcctctcg
     1921 ctttacctac aacgaccgcc acgagcgcat tgtcatcgga gtcagtcgca acattcagga
     1981 acgcaagctt gccgaggttc gcatgaaagc ctcgcaagag gcactgcagt cgagtgaaga
     2041 tcgatacaaa gccctgttgc aatctgtccc cgacctgctg tttctgctcg atcgtcaagg
     2101 cgtttttcgt gactaccatg ccaatgatcc ggcaaaactc atggtcaaac cagaacagtt
     2161 cctgaaccgt cctgtcaacg agatccttcc ccctgaaatc gcagcgcaga cgatgcagct
     2221 tctcgcgacc ctgtttgaaa cgggctctca tcagcagtat gaatatgcgc tccatcttcc
     2281 cgacggcaag cggtttttcg agaatcgctt gtttccctgc ggcaaagatc tggctttgtc
     2341 gatcattcgc gatatcaccg atctcaaaac ctctgaggaa gagaagctgc agcttttaca
     2401 acaggtacat cagtcacaga aactcgaaag cctcggcgtt ctcgccggtg gaatcgccca
     2461 cgatttcaac aatattttaa tgggtattct gggcaacgtg gagctcgcgg aagttgacgt
     2521 gaaacctggt tcgccagttc tctcacgtct gggaaagatc actgccgcag cgcagcgagc
     2581 cgccgaactc tgccgccaga tgctgacgta ttcagggtca agcagcctat cctgcgaaag
     2641 catcgtgctc ccgcttctcg tgccagagat cattcgcctg ctgaaatcga acattcccgc
     2701 gaaaacggct gtgagactcg atttttccac cccgctgccc ccgattttcg ccgatccgta
     2761 tcagattcgc cagatgttgc ggcacctgat catcaatgcc agcgaatcca tcacccacga
     2821 tcatggatta atcatggttt ctgccgtgag tcgtcggtac accagcgacg atctggccgc
     2881 aaccgaactg gccccaacat tgccggcagg tgagtatatc gccgtcagtg ttgaagataa
     2941 cggatgcggt atgagtgcgg aaacccgtcg acgcatgttc gagccgttct attcgacgaa
     3001 gttcgtcggt cgcggacttg gtctggcctc ggttctcggc atcgtccgcg cccacagcgg
     3061 cgcgatcatc atcgattcac aacccgaaaa aggcacccgc atccaggtgt tactacccac
     3121 ggcaaaaggt cttcaaaaca gcgatgttcc ctcgacacct ccggcatcct ctcctcgccc
     3181 gaccatcctg ttcgcggatg acgaggaaag tctgcgcgac atcgctcagg tgactctgca
     3241 gcgtctcgga taccaggtat tgctggcacg caccggacaa gaagccatcg acttgtacaa
     3301 gacccatcgg caggaggtgc gggtcacgat actcgatctc tcaatgcccg gaatggacgg
     3361 tattcaaacg ctcgatgagc tgcgtcatct tgatccaaac gcgctggtca tcatcgccag
     3421 cgggtacagt gaggaagaca ttcgcgatcg cttcgtaagc caggcaccct ctgccgttct
     3481 gcagaaacca ttcaccatga gtcagctgaa aacagtcttt gcccggctca acctgcaggt
     3541 tctttttcag ccctcagtca gaacctgaag aatctgacga ctgatctgta cccgtatgtt
     3601 gggaatgtga gtcagtcgcc acaatagttt tgtcttgcga gagtggagaa ttgatctttt
     3661 tcgcccagag acgattcgcg aggaatatca gacacgcgcc caacagcaac cccagcagat
     3721 gaccacacaa gacatcggcc ggataatgca cgccgacgta gatgcgtgaa taaccgacca
     3781 tgaccgcaat cacccagacc ggcagaccgg cccgccaccc agcttcgagc cagacaaccc
     3841 ccgcaacgcc cgcggtattc gccgcatgcg acgaaggaaa cgacttgctc gtcttgcagg
     3901 gaatcagcag gcgcgcgttc ggttccgcac agcaggggcg ggcgcgacga atcagtggtt
     3961 tgatgactgc cgaactcatc tgatcggcac cgatgacgat caacaccagc aacaccgcca
     4021 accttcggtg agcgccattc cggcccagca gcagccagaa ccagatcgcg gcgagaaacg
     4081 gcagccagtt tttctgatcg gtgatgatcg gcatggcata atccagccag gggtgggtca
     4141 acccgccatt cagcagatga aacagctgtt gatcgaagtt catgctcagc agccgtgatc
     4201 gcgatcagtc atacccaccg gtatgcgtcg catcaagatt atcgaaacgc gcgaactcag
     4261 agaaaaagcc cagttcgaat gagccgaccg gaccgttgcg ctgcttggcg atgatgacct
     4321 cggccttatt gcgcgactct tcctttttcg gattgtaata gctgtcgcga tacaaaaaca
     4381 gcaccacgtc ggcatcctgc tcgatagcac cggattcgcg caaatcggaa agaatcggtc
     4441 gtttgtccgt acgagtttca acaccgcgtg agagctgcga caggcagata atcggcactt
     4501 gcaattcgcg cgccagtcct ttgagacttc gtgatatcgc cgaaatctcc tgttgtcggt
     4561 tctcaccgcc tgatccactc atgctatagg tgatcagctg caggtagtcg atgacgatca
     4621 taccaatctt ctcctgcgcc cagcatcggc gagccttgct gcgaatctcg agcagcgggg
     4681 catttggcgt gtcatcgaga aacacgggca gcacgtcgag catacccgag gtcatggcta
     4741 gttttgtcca gtcttctttg gccagatacc ctgacctgat cttctgtcca tcgatgcgcg
     4801 cttcagaaca gagaatacgc cgcatcaact gcgaccggga catttccaga gaaaagatca
     4861 gtacgccgcg gcgttccgga tgcagctgcg cggcattcac cgcaatattg agacagaacg
     4921 ccgtcttgcc catggaaggg cgggcggcaa cgataatcag atcgctgggc tgaaaacccg
     4981 aagtgtattc atcaagtccc ttgaaaccgg ttggtactcc cgtaatcggt tgtttgcgct
     5041 cgcagatcat ctcgagctct tcgtaggtat ccttcagcag atccttgatg gcgatatagg
     5101 ggcgcgtggc tcgatgctgg ctgagctgca gaaccttctg ctcggcttca tccaacagga
     5161 gttccgcctg ggcctgaggc tcataagcgt cgcgcgcaac ttctccggtc accgagatca
     5221 atcggcgcag ca
//
LOCUS       DAORHD010000145         5226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16449_length_5226_cov_4.507639, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000145 DAORHD010000000
VERSION     DAORHD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16449_length_5226_cov_4.507639"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKO06_04405"
     CDS             complement(<1..601)
                     /locus_tag="PKO06_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68917.1"
                     /translation="MMQLRGRRGFSIPLAILGGGMIAIVMTALSFYSQGEMRSVRHML
                     DSTTGEYLAYSGLIWSYDKLCREGRWYQPAENSIKERGHQWQNSWKGTPDLAIPGNGN
                     AGGPQISVFFDEIRSTHTLKLPGKTINPGNATPGLPLLDHIDSLPLDRPDGRIEQAKR
                     AFDRLPAGLTHFLIHASADTPELRAITPDAPSRIADYQAF"
     gene            complement(613..1110)
                     /locus_tag="PKO06_04410"
     CDS             complement(613..1110)
                     /locus_tag="PKO06_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68918.1"
                     /translation="MTFIETIVYLVVFSLFLAVVMSAFTWYRNSQQGTVRLDVLHQLR
                     QGMALLSEDLSYGSGILFPPESGPGDPASHQISYRNAINEQMLVYRNGQGELVRANLT
                     RKARGQKYLQVLARQVTRFDVKRPGVWYVEYQLSGRQVVQPSQGPEMPEYHLIESTRI
                     RNVLK"
     gene            complement(1152..1655)
                     /locus_tag="PKO06_04415"
     CDS             complement(1152..1655)
                     /locus_tag="PKO06_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68919.1"
                     /translation="MTIRSRSGRAGFSLLEVLIGMGLFLVILASLVRLFTEVTMPQQG
                     MIRDYAMVMGLSERFVNGLIEDIMKGDPPPETPGGTELDVTESVIEVAGHDPTFAKIF
                     TGGAGKEATELTVNFKALLTVQDLGKTLGSDIGSPDGRYFQLHFRCTWGPQRQHSYAL
                     TTAAMRR"
     gene            complement(1652..2008)
                     /locus_tag="PKO06_04420"
     CDS             complement(1652..2008)
                     /locus_tag="PKO06_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV68920.1"
                     /translation="MAFSLAIEAHAPASVLRCCGYVDIEAARQIRAQIQKLQQQGKRI
                     FILDFSQTPVVNSAALGELIEVVSRALEEPELRFLVSGLAPTCQSSFRIVGIFHCAAE
                     FSDVAEALASLTPVKR"
     gene            complement(2026..4293)
                     /locus_tag="PKO06_04425"
     CDS             complement(2026..4293)
                     /locus_tag="PKO06_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68921.1"
                     /translation="MQKAIVRVTIRNIMAPPLSSKHIEHLVFDLSSSERNVRLAAIFQ
                     LGTQLATEEVRQALTEHAQQESDPEVAFYLEQALARGSDTGPQVDKAPTSTTEIPDLR
                     TLPPERYRELLPPVRRLPPEQRVAKIEELLSLNCSSHLAEALLGLGQEFPFRALTDTH
                     FRRLLFHANPGTSVRVLYLAGRHAPERVLAHVPDLLQRPSLLTRALALRLLHRYLPDQ
                     ALRLLEEFLDHPEPAQRGIGVGLLFQFPFEEVAGAVLRLLESGHLPGHSHEMIVTLIR
                     SNPNAGFARRLAYLATNHPDQAELQTLAEAAADALILSGLEAGPLPVLSRRLREAAEQ
                     EAMTALTLPASPDAAPVASAAAPPPVTAATTAPPSATAGTELTALSLENLTSAPQVLA
                     ALLPLAERRERANELTLPAFAALQKHGVSDSRFHDWGESLLEHSPEALLVQVIRYLER
                     QAPRRLVSHLPLLAFHESPFVASQAVRILRRQEGSAFLKRLDVWLKDPDPRALRAGLA
                     GLLQMDFNRVRPLVIRHLRTNPRQEALEGIGRMIRMNPDHRLIAELHTLAAATSTAKR
                     REFLEHLARDCRAELQRLYGATGEPTALHHLRELWADQVEVRLNDLLTRLQSVHLSAS
                     ESLQEDPGRWLRGIALLIGIVALLLASSLSESPFPTVPASSSSTGKPVVIKRATEQAR
                     LSSTVPIIGTLEAYDPLTRTWKFRTADGGLHKVRFSEPSRRLEPGKRISGTVHFLETT
                     PLGYNLYLVHQYQLL"
     gene            4288..4872
                     /locus_tag="PKO06_04430"
     CDS             4288..4872
                     /locus_tag="PKO06_04430"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV68922.1"
                     /translation="MHASSPEKLADSGVFAHNEAVGDRHFHYSGEELVAVRPEINNSI
                     LKLSNEHKVFLDVLEKLERILLETDNRKMLADLEACTEHLQEQLAAHFSFEENVLFKA
                     FLVGIPTDRTVEKVLGLVKQHGVMLNHLQYWRGLAAVHHAENSAQIRFQHQMMVHFVD
                     LLKVHVKTEIEEIYPMIAQNPRCLQLIATMTVDT"
     gene            4910..>5226
                     /locus_tag="PKO06_04435"
     CDS             4910..>5226
                     /locus_tag="PKO06_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV68923.1"
                     /translation="MISSRVLARLLLLILLGACVSASAERALSPDTGRLIPVRLALKW
                     RHQFQFAGYYAALMNGYYRDEGLEVQLLEGGLDGTPAEAKLMRGEVELAVVGIAIFRH
                     GNRR"
BASE COUNT         1074 a         1450 c         1692 g         1010 t
ORIGIN      
        1 tgaaggcctg atagtcggcg atgcggctgg gggcatcggg cgtgatggcc cgcagctcgg
       61 gggtatcagc cgacgcgtgg atcaggaaat gcgtcagccc cgccggcagg cggtcgaagg
      121 cgcgcttggc ctgctcgatg cggccgtcgg gccggtcgag cggcagcgag tcgatatggt
      181 cgagcagcgg cagacccggt gtggcgtttc ccggattgat cgttttgccg ggaagtttca
      241 gcgtgtgcgt ggagcgaatc tcgtcgaaga acaccgagat ctgaggcccg ccggcgttgc
      301 cgttgccggg gatggccaga tcgggtgtcc ccttccagct gttctgccac tgatgacccc
      361 gttctttgat cgagttctcg gctggctgat accagcgtcc ctcgcggcag agcttatcat
      421 acgaccagat caggcccgaa tacgcgagat actcaccggt cgtgctgtcg agcatgtgac
      481 gaaccgatcg catctcaccc tgcgagtaga aggacagagc ggtcatgacg atcgcgatca
      541 tgcctccgcc gagaatcgcc agcggtatgg agaaccctcg tcgccccctg agctgcatca
      601 tatcaacctc cgttacttga gtacgttgcg aatccgcgtg ctttcgatca gatgatactc
      661 cggcatttca ggcccctggc tgggttgaac aacctgtcgt ccggaaagtt gatactcgac
      721 gtaccagact ccgggccgtt tgacatcgaa tcgggtcacc tggcgggcca gcacctgcag
      781 atacttctgc cctcgggcct tgcgggtgag attggctcgg acaagctctc cctggccgtt
      841 gcgatagacg agcatctgct cgttgatggc gttgcgatac gagatctgat gactcgcggg
      901 atcacccgga ccactctccg gcggaaacag gatgcccgag ccgtaggaaa gatcttcgga
      961 aagcagagcc atcccctggc gcagctggtg cagcacatcc agccgcacgg ttccctgttg
     1021 cgagttgcga taccaggtga acgcgctcat caccaccgcg agaaacagag aaaaaacaac
     1081 cagatagacg atggtttcga taaaggtcat tccgcttctc gaacgcgata tggacaaatg
     1141 gcgatttgta ctcatcggcg catggccgcc gtggtgagag catacgagtg ctgtcgttgc
     1201 gggccccagg tgcagcgaaa atggagttga aagtaccggc cgtctggaga gccgatatcc
     1261 gaaccaagcg tctttccgag atcctgtacc gtgagcaggg ccttgaagtt gacggtcaat
     1321 tcggttgctt ccttgccggc gccgccggtg aagatcttcg cgaacgtcgg gtcatggccg
     1381 gcgacttcga tcaccgactc ggtgacgtcg agttcggtgc caccaggggt ttcgggcgga
     1441 ggatcgcctt tcatgatgtc ttcgatcagg ccgttcacga aacgctcaga tagccccatg
     1501 accatggcat agtcgcggat catgccctgc tgcggcatgg tgacttcggt gaagagacgc
     1561 accagcgagg cgagaatgac cagaaagaga cccatgccga tcaacacttc gagcagagag
     1621 aagcccgcgc gccctgaccg ggagcgaatg gtcatcgttt gaccggcgtg agcgaggcca
     1681 gggcctcggc aacatcgctg aactccgcgg cacagtgaaa gatgccgacg atgcgaaacg
     1741 aactctgaca cgttggcgcc aatccgctga ccagaaaacg cagttcgggc tcctcgagcg
     1801 cgcgagagac gacctcgatc agttcgccca gtgccgccga attgacgacc ggtgtctggg
     1861 aaaagtccag gatgaaaatt ctctttcctt gctgttgaag cttctgaatc tgggcgcgaa
     1921 tctgccgcgc ggcttcgata tcgacatacc cgcagcagcg gagcacgctc gcgggagcgt
     1981 gggcttcgat cgccagagaa aaagccacgg atggaacctc aacggttaca ggagttgata
     2041 ctgatgaacc agatacagat tataccctaa aggcgtggtt tcgagaaagt gaacggtgcc
     2101 ggagatccgt tttccgggtt caagtctgcg cgatggctcg gaaaaacgca ccttatgcag
     2161 gcctccgtca gccgtgcgga acttccaggt acgggtgagc ggatcatacg cttcgagcgt
     2221 gccgatgatg ggaaccgtgc tgctgagccg ggcctgttcg gtcgcccgtt tgatgacgac
     2281 cggttttcct gtgctggagg aggaggcggg aacggtggga aacggggact cggaaaggga
     2341 cgacgcgagc agaagagcga caatgccgat gagcagggcg atgccccgca gccagcgacc
     2401 ggggtcttcc tgcagagatt cactggcgct cagatgcacc gactgcaacc tggtcagcag
     2461 gtcgttcaga cgcacttcaa cttgatcggc ccacaactcg cgcagatggt gcagggccgt
     2521 cggttctccg gttgccccat acaggcgctg cagttcggcg cgacagtcac gcgccagatg
     2581 ctcgagaaac tcacgccgtt tcgcggtcga ggtggcggca gccagagtgt gaagttcggc
     2641 gatcagccgg tgatcagggt tcatgcgaat cattcggccg attccctcta gagcctcctg
     2701 acgcggatta gtgcgcaggt gacggatgac cagtgggcgc acccggttga agtccatctg
     2761 cagcaggccc gccagtccgg ctcgcagagc ccgcgggtct ggatctttca gccagacatc
     2821 gagacgtttc agaaatgccg agccttcctg ccgccggaga attcgcaccg cctgtgaggc
     2881 cacgaaaggc gattcgtgaa aggccagaag cggcagatga gaaaccaggc gccgtggggc
     2941 ctgccgctcg agatatcgaa tcacctgcac gagcagtgct tcgggggagt gctcgagaag
     3001 tgattcaccc cagtcgtgaa aacgggaatc gctgacgccg tgtttctgca gagccgcaaa
     3061 tgccggcaga gtgagttcgt tcgcgcgttc gcgacgctcg gccaggggaa gcagcgcggc
     3121 gagaacttgc ggcgccgacg tgagattttc cagcgacaag gcggtgagct cggtgcctgc
     3181 cgtggcggaa gggggggcgg ttgtggcggc cgtgacggga ggaggggcag cggcagacgc
     3241 gaccggtgct gcgtctgggc tggcaggtag agtgagcgcc gtcatcgctt cctgctcggc
     3301 cgcctcacga agccgacgac tgagtacggg cagtggtccg gcttcgaggc cgctcagaat
     3361 cagcgcatct gcggcggctt cggcgagagt ctgcagctcg gcctgatccg gatgattcgt
     3421 ggcgagatag gccagacgcc gagcaaagcc ggcattggga ttactgcgta tcagggtgac
     3481 gatcatttcg tgagaatgtc cgggcaggtg accgctttcg agcagacgaa gcaccgctcc
     3541 ggcgacttcc tcaaagggaa actgaaacaa cagcccgacc ccgatcccgc gctgcgccgg
     3601 ttccggatga tcgagaaact cctcgagcag gcgcagagcc tgatcgggca ggtagcgatg
     3661 cagcaggcgc agggccagag cgcgggtgag gagcgacgga cgctgcagaa gatccggaac
     3721 gtgcgccaga actcgctctg gggcatggcg acccgccaga tagagaaccc gcacgctggt
     3781 gcccgggttg gcatgaaaca gcaggcgacg aaaatgcgtg tcggtcagcg ctcgaaacgg
     3841 aaattcctgt ccgagtccga gaagtgcctc ggccagatgt gatgaacagt tgaggctgag
     3901 gagttcctcg atttttgcga cacgttgctc gggaggcagg cgccgcacag ggggcagcag
     3961 ctctcgatat cgttctggcg gcagggtgcg aagatcggga atctcggttg tggatgtcgg
     4021 cgctttgtcc acctgcggac ccgtgtctga gccgcgtgcc aatgcctgtt cgagatagaa
     4081 cgcgacttcc gggtctgact cttgttgcgc gtgttcggtc agtgcctgtc gcacctcctc
     4141 ggtggcgagc tgagtgccga gctgaaaaat ggcggcgagt ctcacgttgc gctcgctgga
     4201 ggagagatcg aagaccagat gctcgatgtg cttggacgag agaggcggtg ccatgatgtt
     4261 ccttattgta acgcgcacga tggctttttg catgcctcat ccccggaaaa gcttgcggat
     4321 tcgggcgttt tcgcgcacaa tgaagccgtc ggtgatcgtc acttccatta ctctggtgag
     4381 gaactcgtgg ctgtgcgccc cgaaatcaat aactcgattc tcaaactttc gaacgagcac
     4441 aaggtgtttc tggatgtact cgagaagctc gaacggattc tgctcgagac ggacaaccgg
     4501 aagatgctgg cggatctgga agcctgcacg gaacacctgc aggagcaact ggccgcgcac
     4561 ttttcgtttg aagaaaatgt cttgttcaag gcttttctgg tcggcattcc gacagaccgt
     4621 acggtcgaga aggttctggg gctggtgaaa cagcacgggg tgatgctgaa tcacctgcag
     4681 tactggcgtg gtctggctgc tgtgcaccac gcggaaaact ccgcacagat tcgatttcag
     4741 caccagatga tggtgcactt cgtcgatctg ctgaaagtgc acgtgaaaac cgagatcgag
     4801 gaaatctatc ccatgattgc gcagaatccg cgctgtctgc agctgattgc gacgatgacg
     4861 gtcgacacct gaccgaagca gtaaagcacc aactgaagaa actccctgta tgatttcatc
     4921 gcgagttctg gcccggctcc tcctgctgat cctgctggga gcgtgcgttt ctgcttcagc
     4981 agaacgggct ctctcaccag ataccggtcg tctcataccg gtacgattgg cattgaaatg
     5041 gcgtcaccag tttcagttcg ccgggtatta tgccgcgctc atgaacgggt actatcgcga
     5101 tgagggcctt gaggtgcaac tgctggaggg cggcctggat ggaactccgg cagaggcgaa
     5161 gctgatgcgg ggggaggttg agctggcggt ggtcggcatc gcaatctttc gccatggcaa
     5221 tcggcg
//
LOCUS       DAORHD010000146         5223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16469_length_5223_cov_3.565596, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000146 DAORHD010000000
VERSION     DAORHD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16469_length_5223_cov_3.565596"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKO06_04440"
     CDS             <1..1140
                     /locus_tag="PKO06_04440"
                     /inference="COORDINATES: protein motif:HMM:NF040246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HNV68924.1"
                     /translation="LIEWYQDVKDASEFMETVKVELFQYHVYVFTPKGDVIELPRGST
                     PVDFAYYIHSDVGHRCMGAKVNGHIVPLDYSLQNGDIVSIITSKSQKGPSRDWLKFVQ
                     STNAKKKIRAWLKKEFREDYIAEGGKEFLDALARAGGDKEMEKFLKTSAFEEEVKNLG
                     FPTVDEMYAGIGAGEVRALQVLHKLFPQLTEKVEKAQAEKVAEKVQAKPDREERKKRE
                     KHGPRVIVGGLDSAMVKISHCCNPIPGDPIFGFITRGRGVTVHRTDCSNARSLMNSPE
                     ARVIDVHWDMGVQIEQTKGYITRLRLETEDRTGMLNSVTTVISGQGVNILAASARTTK
                     KGTGLLEFSVEVRDTSQLQGLIRSLQRLAGVEQVFRVDAGAQGKN"
     gene            complement(1206..1928)
                     /locus_tag="PKO06_04445"
     CDS             complement(1206..1928)
                     /locus_tag="PKO06_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68925.1"
                     /translation="MAHADPAIRLAYRFNPGQRLVYRVTVSGHVSVETPEGVAVNPVR
                     IEMELWQTVSRLEEDRAFIQVLVRSARAFSGSESTPLPEEGQRMSFIMKENGETTYED
                     GASPFQGSEFAQMAFPAHPLRIGDSWEQSPAGGKPGQGRGKTRYTFLRQATSGIHACS
                     VFQADMNLEPASTAGGGEAHGGGSSGEVWFSETLGQVIHTTAKSQFSFTIPIPQTYAL
                     AKTTTALTTEMKLIAASEGKTE"
     gene            2258..2617
                     /locus_tag="PKO06_04450"
     CDS             2258..2617
                     /locus_tag="PKO06_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68926.1"
                     /translation="MPLLDEPQSTGEKVGEFLDETSLDLLLKGSARTRPDADVPVPAA
                     PEPALPAELESMVDLVAANEFETTTSFTESEETTGDTGEATMEILSEAGIAPDLELSE
                     QGSTEASANAPDSSLQE"
     gene            2654..3283
                     /locus_tag="PKO06_04455"
     CDS             2654..3283
                     /locus_tag="PKO06_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV68927.1"
                     /translation="MATLRRKQRRHRVFIKPGFQFRLTLILILVVIIVANIVGALVYG
                     LSIDKLESKLVVEAKLPIDPTQLGQALLPGVIIAELISILITAFICIFVTHTIAGPVY
                     RMERVARSIGEGDLTNFIKLRPKDELKDLADSMNEMTMGLRNKVTSLKEASERVKSGL
                     ERLKSSGKITGNDEVFEAAGNIHKIVDMFVLEREIDDSHADRKEEKEEA"
     gene            complement(3344..4951)
                     /gene="nadB"
                     /locus_tag="PKO06_04460"
     CDS             complement(3344..4951)
                     /gene="nadB"
                     /locus_tag="PKO06_04460"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNV68928.1"
                     /translation="MNIFTQTHQYDVAIIGSGVAGLYSALTIARTTQARIGIFTKGAL
                     VDGNSSLAQGGIASAWADDDDPTEHARDTLAVGAGLNDEDAVRVLVQEGPMQIRSLIA
                     MGTRFDADDEQIRLTREGGHSRRRVLHALGDATGREMVRALVSRLADYPSISVHDHFL
                     SIDLLKSGDSVSGFTGLNLISGHKQLVRCPSIILATGGASAIYPRTTNPAETTGDGIC
                     MAWAIGATLADLEFVQFHPTALAIPGHSSFLMSEALRGEGAILRNHAGEAFMSRYHAM
                     KDLAPRDIVSRAIVDQLKRERVEHVFLDARHLGEAYFASRFPTIFEHLAARGINPGHD
                     LIPIAPAAHYFMGGVLTDLEGRTSVPGLYAAGEVACTGVHGANRLASNSLLECVVFGQ
                     RAARSAIATLSREPGTIAGEAEQSWEVSVPESTDVIRRWIGESLGISRQARDLEPLVS
                     RLGPSCLHRRVCGVEGQNPERLAHRNLAVLAGLIGLSALTRRESRGAHFRTDFPETDP
                     AWHGRLFVTQDQVKFEKLTNRAPAPKS"
     gene            complement(4948..>5223)
                     /locus_tag="PKO06_04465"
     CDS             complement(4948..>5223)
                     /locus_tag="PKO06_04465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV68929.1"
                     /translation="PNPTEALMVDDLRDSRMTLTEILGTPPRAIAWPYGKFNNALRRI
                     AQDQGFRLHFTSISGYNEPGVNPFSIKRVPITNRDTALHVLRKARGK"
BASE COUNT         1137 a         1395 c         1609 g         1082 t
ORIGIN      
        1 ctcatcgaat ggtatcagga cgtcaaagac gcctctgagt tcatggagac cgtgaaggtc
       61 gagctgtttc agtatcatgt ctacgtcttt accccgaagg gcgatgtcat tgagttgccg
      121 cgcggcagca cccccgtgga tttcgcctac tacattcaca gtgatgtcgg gcatcgctgt
      181 atgggcgcta aagtcaacgg gcacatcgtt ccgctcgact actcactgca gaacggcgat
      241 atcgtctcga tcatcaccag caagtcgcag aaaggtccgt ctcgcgactg gctcaaattc
      301 gtgcagagta ccaacgcgaa aaagaagatt cgcgcatggc tgaagaaaga gttccgcgaa
      361 gactacattg ccgagggggg gaaggagttc ctcgatgcgc tggcccgtgc cggtggcgac
      421 aaggaaatgg aaaagttcct gaagaccagc gcctttgaag aagaggtgaa gaatctcgga
      481 tttcctaccg tcgacgagat gtatgccgga atcggcgcgg gagaagtacg tgcgttacag
      541 gtgctgcata agctgtttcc gcagttgacc gaaaaggtcg aaaaagccca ggccgagaag
      601 gttgccgaga aagtccaggc caaaccggac cgagaggagc gtaagaagcg ggagaaacat
      661 ggtccgagag tcattgtcgg cggactcgac tccgcgatgg tcaagatttc ccactgctgc
      721 aatccgattc cgggcgaccc gatttttggc ttcattacgc gtggtcgcgg cgtcacggtt
      781 catcgtaccg attgttccaa tgcgcgctct ctgatgaaca gtccggaagc tcgggtcatt
      841 gacgtccatt gggatatggg cgtacagatc gaacagacca aggggtatat cacgcgtcta
      901 cgcctggaaa cagaagaccg caccggcatg ctcaactccg tgaccacggt gatttccggg
      961 cagggggtca atatcctggc ggccagtgcc cgcaccacga aaaagggaac cggtttgctc
     1021 gagttttccg tcgaggtgcg cgataccagc caattgcagg ggttgatccg ctcgctgcag
     1081 cgtctggccg gggtcgaaca ggtgtttcgc gttgatgccg gagctcaggg aaagaactga
     1141 accgtcgtta gccgcttgag atgacggctt ttcggtaaac aaactcggac aaaaaccgta
     1201 gggggttact ccgtttttcc ttcgctggcg gcgatcagtt tcatttccgt cgtgagtgcc
     1261 gtcgtggttt tcgccagggc atacgtctgc ggaatgggaa tcgtgaacga aaactgactt
     1321 ttcgccgtcg tgtggatcac ctggccgagg gtttcactga accacacctc gccgctcgaa
     1381 ccgccaccat gagcctcgcc accgcccgcc gtgctggcgg gttccagatt catgtccgct
     1441 tgaaataccg agcaggcatg aattccggaa gtggcttgcc gtaaaaacgt gtatctggtt
     1501 ttcccgcggc cctggcctgg tttgcctccg gccggacttt gctcccagga atcgccgatt
     1561 cgcagggggt gcgcgggaaa agccatttgg gcgaactcgc tgccttgaaa cgggctggca
     1621 ccatcttcgt aggtggtttc tccgttctcc ttcatgatga acgacatgcg ctgcccttct
     1681 tcgggcagtg gggtcgactc ggagccgcta aaggctctcg ctgaacgcac gagaacctga
     1741 atgaacgcac gatcctcttc gagacgggaa acggtttgcc agagttccat ctcgatacgc
     1801 accggattca cagccactcc ttccggagtt tcgacgctga catgaccgga aaccgtgact
     1861 cggtacacca ggcgctggcc ggggttgaaa cggtaggcca gacgaattgc gggatctgcg
     1921 tgtgccatcg acttctcctc agaaaaacag tgcaggactg acaacgccag acagagcaga
     1981 atacggataa cgagatgcgg catgttgagt gggcttcatc acaggactgt taagaaataa
     2041 tgggggattg gccgaagaga agtatagacc acttgccatc tttctgcaac ggagaccaac
     2101 cccggtgatg ccattaggat tgcctcggaa tttcttgaca gccccagacc gttccattag
     2161 aatgatgccg cgagcccgat ttggtctgcc ttcttgggcc ttggcggtcc tggcggtcac
     2221 ggtgttcggg tgttggtttc cgttgtttgg tgaaccggtg cctctgttgg acgaaccaca
     2281 gagcacaggc gaaaaggttg gcgagttcct ggacgaaacg tctctcgatc tgcttctgaa
     2341 aggatcagcc cgcaccagac cggatgctga tgtgcccgtt cctgcggccc cagagccagc
     2401 attgcccgcg gagctggaaa gcatggtcga tctcgtggcc gcgaatgaat tcgaaacaac
     2461 gacctcgttt acggaatcgg aggagacaac cggagatacg ggcgaagcga caatggagat
     2521 cctgagtgaa gccgggattg cgcccgactt ggaactgtcc gagcagggtt ccacggaagc
     2581 atcagcaaac gccccggact ccagtctcca ggaatagcag catcgccggc ggaatttacg
     2641 atcgggaggg aatatggcaa cgctacgtcg gaaacaacga agacatcggg tatttatcaa
     2701 gcccgggttc caatttcgtt tgaccctgat cctcatcctt gtggtgatca tcgtggcgaa
     2761 catcgtcgga gctttggtct acggcctttc gatcgacaag ctcgagagca aactggtggt
     2821 tgaagccaaa ttgcccatcg atccgacaca gttggggcag gcgttgctgc cgggggtcat
     2881 cattgctgaa ttgatcagca ttctgattac ggcattcatc tgtatttttg tcacgcatac
     2941 gatcgccggg ccggtatatc gtatggaacg cgtggcgcgc agcatcggtg aaggggatct
     3001 cacgaatttc atcaagctgc gccccaaaga cgaactcaaa gatctggctg actcgatgaa
     3061 cgagatgacc atgggtctgc gcaataaggt aaccagcctg aaagaagcat cagaacgtgt
     3121 gaagtctggg ctcgaacgct tgaagtcttc gggcaagatc acgggaaatg atgaagtctt
     3181 cgaggctgcg ggcaatattc acaagatcgt cgatatgttt gtgctcgagc gcgagatcga
     3241 tgacagccac gctgatcgta aagaagaaaa ggaagaagcc tgagagcttt ttccagaatc
     3301 aagacctgcc aatccgcccg gggtaacccg ggcgggttct tttttaggac ttgggagccg
     3361 gtgcgcggtt ggtaagcttc tcgaacttta cctggtcttg ggtaacgaag aggcgaccgt
     3421 gccaggctgg atccgtttcg gggaaatccg tgcgaaagtg cgcgccacga ctctctcggc
     3481 gagtcagagc gctcaagccg atcagaccgg caagcactgc caggttgcga tgagcgagtc
     3541 gctcagggtt ctgaccctcc acgccacaaa cgcgacggtg caggcaagaa ggccccagtc
     3601 gtgacaccag cggttcgaga tcgcgggcct gtcggctgat gccgagactt tcgccgatcc
     3661 agcggcggat cacgtccgtg ctttccggaa cggagacctc ccagctttgc tcggcctcgc
     3721 cagcgattgt gcccggttcg cgggacagtg tggcgatggc cgaacgtgcc gcccgttggc
     3781 cgaagaccac gcattcgaga agactgttcg aggccagtcg gttcgcgccg tgaactccgg
     3841 tgcaggcaac ttcgccagct gcgtacaagc cgggaacgga ggtgcgacct tccagatcag
     3901 tgagaacgcc gcccatgaag tagtgcgcgg cgggcgcaat cgggatcaga tcgtggccag
     3961 ggttgatacc gcgcgcggca agatgctcga aaatggttgg gaaacggctg gcgaagtagg
     4021 cttcaccgag atgccgggca tccagaaaca cgtgttcaac gcgttctcgc ttgagttggt
     4081 caacgatggc acgcgaaacg atgtctcggg gagccagatc tttcatcgcg tgataccggc
     4141 tcatgaaggc ttcgccggca tggttgcgca gaatcgcgcc ctcgcctcgc agggcttcgc
     4201 tcatgagaaa ggaggagtgt ccgggaatag ccagagccgt gggatgaaac tgcacgaatt
     4261 cgaggtcggc gagggtcgcg ccgatggccc aggccatgca gatgccgtca cccgtggttt
     4321 cggccgggtt cgtcgtgcgc ggatagatgg cgctggcccc gccggtagct agaatgatac
     4381 tgggacatcg tactagttgt ttgtggccgg atatcagatt cagtccggtg aatccgctca
     4441 cggagtcgcc agatttcaga aggtcgatag ataaaaaatg atcgtgcacg gagatggagg
     4501 gatagtctgc cagacggctg acgagagctc gtaccatttc acgcccggtg gcatccccga
     4561 gagcgtggag aacccggcgg cgtgagtgac cgccctcgcg cgtcaggcgt atctgctcat
     4621 catcggcgtc gaatcgcgtg cccatggcga tgagacttcg gatctgcatc ggcccttcct
     4681 gtacgagaac tcgtaccgcg tcctcgtcgt tcaaacccgc tcccacggcg agggtgtcgc
     4741 gcgcgtgttc ggttggatcg tcgtcgtcgg cccaggccga ggcgatgccg ccctgggcaa
     4801 gcgaggagtt gccgtcgacc agagctccct tggtgaaaat gccgattcgg gcttgggtgg
     4861 tacgggcaat cgtcagggca gagtacaagc cggcaacgcc tgatccgatg atggcgacgt
     4921 catattgatg agtttgagtg aagatgttca ttttcctctg gctttgcgaa gaacgtggag
     4981 ggcggtgtct cggttggtga tcggcactcg cttgatggaa aacggattca cgcccggctc
     5041 attgtatccg gagatgctcg tgaagtgcag acgaaacccc tgatcctgag caattcgtcg
     5101 cagagcgttg ttaaacttgc catatggcca ggcaatcgcc cgtggcgggg tgccgagaat
     5161 ttcggtcaga gtcatgcgtg agtcacgcag gtcgtcgacc atcagagctt cagtcggatt
     5221 ggg
//
LOCUS       DAORHD010000147         5209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16534_length_5209_cov_3.728948, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000147 DAORHD010000000
VERSION     DAORHD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16534_length_5209_cov_3.728948"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKO06_04470"
     CDS             <1..880
                     /locus_tag="PKO06_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV68930.1"
                     /translation="RAVLIVFFIAIFVENLGVAPYTRVLRDMYSERGRGMAMSYVRAW
                     SGGCQIIGSFLGGAMLDRNYGWLVFALAGICGAISSWNFRRIFAGRESPLFVAQAMGV
                     REVTSSLRASEGFFWMNLTIMLFGLGNLMVLGVLPTLLVNRFNISNFALGNLNALTFI
                     VTVFSYLLLARYVSNYGAKNGLLLGMTAGVLNPWLFLLSPSVHYLTVPYAFSGLMYAA
                     FDISWMLLIISYVPTDQIGRHAAVYTFLMGIRGVTAMLATNLALPYVDPALLLSIGGI
                     FTALGLGIGVWKRSRW"
     gene            882..2717
                     /gene="uvrC"
                     /locus_tag="PKO06_04475"
     CDS             882..2717
                     /gene="uvrC"
                     /locus_tag="PKO06_04475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNV68931.1"
                     /translation="MIPRDELRRRLAHLPQKPGVYIMYDREGTVIYVGKSRSLKNRVR
                     SYFVGRGHDSKTDRLVHSVTQFEFIVTTNETEALILENNLIKKYKPHYNVRLKDSKTH
                     PYLMISLSEPYPRLAKVRRVKFNDGNRYFGPFPDESGLRLIREILTKQFHLCDCTTPF
                     KPGKPAKRACLNHHLGYCPGPCIEAITVDDYGKHVQRAIDFLSGRAALDVDGMKEQMQ
                     EHARNFRYEAAAELRNLIQALDGFFTRQKVEMTRPIDLDIWGTAFSHDLLVASIFFVR
                     AGKLLGHRTIEAEKEPGSSEEQQVGTLMSRFYEQNLIPQKIVVTVRPSPLEPLLSYLK
                     TLSGREIRVGRPVRGEKRRLLNMADENAAEILRNLKSDDHERVAENVIDLERRLGLPR
                     TPRRIECIDISHVQGADTVASLVCAINGAPRRGEYRLYHIKTVVGVDDPASIAEVTRR
                     RFERLKREGTSLPDLFVVDGGITQVRAAREVLRDLEIDVPLYGLAKREELLVHPDGSE
                     IRLPVTSPALRLLIKLRDEAHRFAHSFQRKTHAKRVMRSSLLSLPGVGPKTLQTILTV
                     YGSLERAAAATFEEFQQRTRLSTRVATLVWNSLRPVGSIRPETEE"
     gene            2769..3722
                     /locus_tag="PKO06_04480"
     CDS             2769..3722
                     /locus_tag="PKO06_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68932.1"
                     /translation="MSRYSTFINRRTAVSPRGVSQPGLLYPLFLLFVLGSVVAAASQT
                     DERLSVAFQRAFADGEQFRQTELFACLGYTASQSRAMAEITPRPAGLIVTAVPAIASG
                     TYEQINVVASAVRYYGLTIAEASFTFPLVRLDEHALSEGRLVLREVPEVLIETRVSAA
                     DLLRVFPFSNRSRQLSNLRLSIAEKHIDLRGNVRRGMFLVAFHIRGKPVLDGAKKVDF
                     SCSRLVLNGIAMPRAAIRAMFSHVNPVFDATKTWLNLELLSIHTEPGTVCTRVKLCQA
                     QPATERSNDVVNASDTTVGRNANSGLASHAQRLTPSSERNH"
     gene            3724..4557
                     /locus_tag="PKO06_04485"
     CDS             3724..4557
                     /locus_tag="PKO06_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV68933.1"
                     /translation="MPSPRRIHRISQALHRRQDDLVVAIERVHDRHNISAILRSADAV
                     GVGHVIWEPDPRQNEEPINPEISKGTERWVQLKVTKKLTRDLRALKKKGYQVAATHLS
                     HRAVDFRSLDWTKKWVVILGNEHAGCSEDVLAEADENIILPMMGLVQSLNVSVATAVL
                     LYEIQRQRENAGMYQRQLPKRTVQKIMKAWRLNDEGYTIEHLLNPPAPEDELPPAHDR
                     HSDGRLRGFSRVRRREERARRRAAVAPAIDAQPAAPTNADRPMKRAPSSAKKSRPRSR
                     R"
     gene            4557..4952
                     /locus_tag="PKO06_04490"
     CDS             4557..4952
                     /locus_tag="PKO06_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68934.1"
                     /translation="MVIVKALIVWLLICAAEIAHGILRMRFLAPRVGKTRSDQIGVFT
                     GSLIILTIVWLLFPWLGASQKFEQVLIGLAWTIGMLCFEFGFGRWYLRLPWDRLTEAY
                     DPRRGGRMLLGMAFLAASPCLVAWLRSGL"
BASE COUNT         1025 a         1505 c         1531 g         1148 t
ORIGIN      
        1 gcgggccgtg ttgatcgtct tttttatcgc catcttcgtc gaaaaccttg gcgtggcgcc
       61 gtatacccga gtcctgcgtg acatgtacag cgaacgcggc cgtggcatgg ccatgagtta
      121 tgtgcgcgcc tggagcggcg gctgtcagat catcggaagc tttctgggcg gcgcgatgct
      181 cgatcggaac tacgggtggc tggtctttgc ccttgccgga atctgcggtg ccatttcgtc
      241 gtggaacttt cgtcgcattt ttgcgggtcg cgagtcgccg ctctttgtgg ctcaggcgat
      301 gggggtccgt gaggtgacct cgtcactgcg cgcgagcgaa ggctttttct ggatgaacct
      361 caccatcatg ctgttcgggc tcggcaatct catggttctc ggtgtgctgc cgactctgct
      421 ggtcaatcgc ttcaacatct caaacttcgc cctgggaaac ctgaatgctc tgaccttcat
      481 cgtgactgtc ttcagttacc tgctgctggc ccgatacgtc tcgaactacg gcgcgaaaaa
      541 cggtctgctg ctgggcatga ccgctggagt cctgaatccg tggctgtttc tgctgtcgcc
      601 ttcggtgcac tatctcacgg tgccgtatgc cttcagtggt ctgatgtatg cggccttcga
      661 catcagctgg atgttgctca tcatctccta tgtaccgacc gaccagatcg gacgacacgc
      721 cgcggtctac acctttctga tgggcatacg tggtgtgacg gccatgctcg cgaccaacct
      781 ggctctgccg tatgtcgatc ccgctctgct gctgtcgatc ggtggtatct tcaccgcact
      841 cggcctgggc atcggggtct ggaaacggag ccgctggtga catgattccg cgcgacgaat
      901 tgcggcgccg actggcgcat ctgccgcaga aacccggggt ctacatcatg tatgatcgcg
      961 agggcacggt gatctacgtc ggcaaatcac gctccctcaa gaatcgtgta cgttcctatt
     1021 ttgtcggtcg cggtcacgat tccaagaccg atcgactggt acactcggtg acgcagttcg
     1081 agttcatcgt gacgaccaac gagaccgaag cactgattct cgaaaacaat ctgatcaaaa
     1141 agtacaagcc gcattacaac gtgcggctca aggacagcaa gacgcacccg tatctgatga
     1201 tttcattgtc cgagccgtat ccacgtcttg ccaaggtcag acgcgtcaaa ttcaatgacg
     1261 gaaatcggta ctttggaccg ttccctgacg agagcggcct gcgtctgatt cgcgaaattc
     1321 tgaccaaaca gtttcatctc tgtgactgta cgaccccgtt caagcctgga aagccggcga
     1381 agcgggcctg cctgaatcat catctcgggt attgtcccgg cccctgcatt gaggcgatta
     1441 cggtcgatga ctacgggaaa cacgtgcagc gggccatcga ctttctgtcg ggccgcgcgg
     1501 cgcttgacgt cgacggcatg aaggagcaga tgcaggagca tgcccgaaac ttccgctatg
     1561 aagccgcggc cgaactgcgt aacctcattc aggcactcga cggattcttc acgcggcaga
     1621 aggttgagat gaccaggccg attgatctcg atatctgggg taccgcattc agtcatgatc
     1681 tcctggtcgc ttcgattttc ttcgttcgag ccggcaagct gctggggcat cgcaccatcg
     1741 aagccgaaaa ggaacccggt tcgtccgagg aacagcaggt tggaactttg atgagccgct
     1801 tttatgaaca gaatctcatt ccgcagaaga ttgtggtcac ggtgcgaccc agcccgctcg
     1861 aacctctgct ctcgtatctg aaaactctct ccggaagaga aattcgtgtt ggtcgaccgg
     1921 tacgcggcga aaagcggcgg ctgttgaaca tggctgacga gaatgccgct gagattctgc
     1981 gcaacctgaa gtctgacgat cacgaacgtg tggccgagaa cgtcatcgac ctcgagcgcc
     2041 gtctcggttt gccgcgaacc cctcgtcgta tcgagtgcat cgacatctcg cacgtacagg
     2101 gcgccgacac ggtcgcttct ctggtttgtg ccatcaacgg agcaccgcgt cggggagagt
     2161 accggttgta tcacatcaag actgtggttg gcgttgatga tccggccagt atcgccgaag
     2221 tcacgcggcg acggttcgaa cggttgaagc gcgaaggcac gtctctgccg gacctgttcg
     2281 tcgtcgatgg cggcatcacg caggtgcgcg ctgcgcgtga ggtgctgaga gaccttgaga
     2341 tcgacgtgcc cctgtatggg ctggccaagc gcgaagaact gctggtccac ccggacggat
     2401 cagagattcg tctacccgtc acctcccctg ccctgcgcct gttgataaaa cttcgcgatg
     2461 aagcgcatcg gttcgcccac tcgttccagc gcaagacgca tgcgaagcgg gtcatgcgct
     2521 cgtcgctgct gtcgttgccc ggtgtcggac cgaagacgct gcagacgatt ctcaccgtgt
     2581 atggatctct cgaacgagcc gccgctgcca ccttcgaaga gtttcagcag cgcaccagac
     2641 tctcaacccg tgtagcgact ctggtttgga actcccttcg ccccgtcggc tcaatacgcc
     2701 ccgagacaga agaatgactg gcgcgcagga gtgacaacgc atggcgaatc tgctacaaag
     2761 aaggcctcat gagccgctac tcgactttca ttaaccgtcg cactgccgtt tccccgcgtg
     2821 gggtgagcca gcccggtctt ctgtatccgc tgttcctgct gtttgtgctc ggttcggtgg
     2881 tagcggcggc ttctcagacc gacgaacgcc tgtcggttgc gtttcagagg gcttttgctg
     2941 atggtgagca gtttcgccag accgaactgt ttgcctgcct gggctacacc gcatcacaaa
     3001 gccgtgccat ggcggagatc accccgcgac cggctgggtt gatcgtcacg gcagtaccag
     3061 ctatcgcatc gggaacctat gaacagatca atgtagttgc ttcagcggta cgctattatg
     3121 ggctgacgat cgccgaagct tcgtttacct ttcccctggt tcggcttgat gaacacgcgt
     3181 tgtccgaggg acgcctggtg ctccgggagg taccggaagt gctcatcgag acccgtgtgt
     3241 cagccgctga tctgctgcgg gtgttcccct tctcaaaccg gtctcgccag ttgtcgaacc
     3301 tgcggttgag tatcgccgag aagcacattg atcttcgtgg aaacgtgcgt cggggcatgt
     3361 tcctcgtggc gtttcacata cgcggaaaac cggtgctcga tggggcgaag aaagtcgatt
     3421 tttcctgctc ccgcctggtt ctgaatggaa ttgcgatgcc gcgtgccgcg atcagagcca
     3481 tgttcagtca cgtcaatccg gtgttcgatg ccacgaaaac ctggctgaat ctcgaactgt
     3541 tgagtattca caccgaaccg ggaactgtct gcacccgtgt gaagctgtgt caggcacagc
     3601 cggcaaccga gcggtcgaac gacgtcgtga acgcttcaga cacgacagtc gggaggaacg
     3661 ccaattctgg actggcgagc cacgctcaac ggctcacccc atcaagcgaa aggaatcact
     3721 gacatgccct ctccacgccg aattcatcgc atctcgcaag ccctgcaccg tcgccaggac
     3781 gacctggtgg tcgccattga acgcgtgcat gatcggcaca acatctcggc tattctgcgt
     3841 tccgccgatg cggtcggggt tgggcatgtg atctgggaac cggatccgcg acagaatgaa
     3901 gaaccgatca accccgagat ttcgaaggga accgagcgct gggtgcagct gaaagtgacg
     3961 aagaaactga cccgggatct gcgagccctg aagaaaaagg gataccaggt tgccgccacc
     4021 catctcagcc accgggcggt cgactttcgc agcctggact ggacgaagaa atgggtcgtg
     4081 attcttggaa acgagcatgc gggctgttcg gaagatgtgc ttgccgaagc tgatgaaaac
     4141 atcattcttc cgatgatggg gctggtacaa agcttgaacg tttcggtggc gacggcggtt
     4201 ctgttgtatg agattcagcg ccaacgcgag aacgccggca tgtatcagcg acaactgccg
     4261 aagcgcacgg tgcagaagat catgaaggcc tggcgtctga atgatgaagg ctacacgatc
     4321 gaacatctgc tgaatccccc ggcccccgaa gacgagttgc cgccggcgca cgatcgacac
     4381 agcgacggca gactgcgcgg gttttcgcgc gtgcgccgcc gggaagagcg tgccaggcga
     4441 cgggcagcgg tcgcacccgc gatcgacgct cagccggcag ctccgacgaa cgctgacaga
     4501 ccaatgaaac gtgcgccgag ctccgcgaag aaaagtcgac cccggagtcg tcgctgatgg
     4561 tgatcgtcaa ggccttgatc gtctggttgc tcatttgtgc tgccgaaatt gcgcatggca
     4621 ttctgcgtat gcggtttctc gctccccgtg tgggtaaaac gcggtcagat cagatagggg
     4681 tgttcacggg ttcgctgatc attctgacga tcgtctggct tctctttccg tggctgggcg
     4741 cgtctcagaa attcgaacag gtactgatcg gcctcgcctg gaccatcggc atgttgtgct
     4801 tcgagtttgg cttcggtcgc tggtatctgc gcctgccgtg ggaccgcttg accgaagcgt
     4861 atgacccgcg ccgcgggggt cgcatgttac tgggaatggc ttttctcgcc gcttccccat
     4921 gtctggtcgc ctggctgcgc tcaggcctgt aaccattccg ggatcacatt ccgcccgtgg
     4981 tgccagatgc cagccgttga tgggaaatct gcgaaactct gatgaactcg ataccatagc
     5041 gtcttctgaa ttcatagaac gaaactgttt cgacaagacg tattctcaat catcgggtcg
     5101 ggaaaacttc acgttgacgg cttcgccggg ctgcaaccgc ccgtcgaaat cccgaagttc
     5161 ctgccacaga tgggttcgca tgattgcctg ggcgtcacgc accagggct
//
LOCUS       DAORHD010000148         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16573_length_5202_cov_4.085681, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000148 DAORHD010000000
VERSION     DAORHD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16573_length_5202_cov_4.085681"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1062)
                     /locus_tag="PKO06_04495"
     CDS             complement(79..1062)
                     /locus_tag="PKO06_04495"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNV68935.1"
                     /translation="MRQGFHVVESAASPDSPNGFFRRPSVRLSISFATCFLFAIFVLA
                     QRPARLIAASAAPSIEVDGVRLQMLSEMMQAIPGATATFALQVTLPADLHLYWLNPGD
                     SGMPPSFTLTLPPGYRVGEIQWPVPTRHEQHGEVLYLYQGTVVFPFEVAIPASVTGVT
                     SFPLRLEVQALLCGEECRPFSASLESTIAVSTQPSVLDPALVTVFSEARLRLPHAQST
                     LRLQAGSTSDRLWLITIEPTPDGPLTEPIQFLPLQAGVIRDTAPQRFQSETATQPAQL
                     LVEQGFASSATHLEGLIIPVGTHTSGPFRTGRAFSVRIPIDSPSDPQVRSQ"
     gene            complement(1161..2018)
                     /gene="rsmA"
                     /locus_tag="PKO06_04500"
     CDS             complement(1161..2018)
                     /gene="rsmA"
                     /locus_tag="PKO06_04500"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNV68936.1"
                     /translation="MGGRPGGPALMQSIRDLLQQLGIDPRKSLGQNFCVDKRLSESVV
                     EALQTQPDDTIWEIGPGLGALTRRLAKQGNPLWCFEMDERLRPHLHETLAPFSQTHVI
                     WGDFLEVDHTPAVPTSGTLIICGNLPYSSGTAMVRRLLEAPPARFRRGVFLLQREVTR
                     KACARPGSDDYSFLSVAVGLYAHSRVGATFSPASFFPNPQVWSSLLLLEPLPLTTEER
                     QRRQRALSLASTAFEQRRKMTVNLLKKRHTVIHRPWEDVFATLRLDAGVRAENLSHET
                     YLALADNLA"
     gene            complement(1988..2299)
                     /locus_tag="PKO06_04505"
     CDS             complement(1988..2299)
                     /locus_tag="PKO06_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HNV68937.1"
                     /translation="MRLDKFLQVSGLIKRRVLGNEACDRGLVNVNGVRAKPTREVKSG
                     DVIDLHLPFREVSVRVLQELTSGTMRKSDRPKYLETLRDEPRKPTQDESWWEDDPVDQ
                     H"
     gene            complement(2365..>5202)
                     /locus_tag="PKO06_04510"
     CDS             complement(2365..>5202)
                     /locus_tag="PKO06_04510"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV68938.1"
                     /translation="EQNCPPGIEWELIGMNSARGVVVPLDSPYVSAAARAIEAGFGRA
                     PVFIREGGSIPVVLAFREKLGVDTLLLERYAEINNVQPRVPASQIVKQEEAQLTPPEF
                     SRQLQKQGLTKTEYQARKHQMLVMQEIFESQTRPMTASAATDAAIEAYYTQNRIRFKK
                     DDEVSFEHVLFSPHGFLSKVTVSDDEVAEYHKIHCNEFQTSKRLSVLHVFINPDDPEY
                     QKQFTPEEPAIRQYYTGNLDKFKKSEQVRARHILIKPRNTFEKKLDTFTANLRDFRFV
                     PATEGQKSEYTFELGISDRKSGINLNFKDIVLVTKDGGRYSPDKESQDKAGSPLEFPQ
                     SGTPSSGNVYGEVCIQLPKDALPAKLEIRDGQSQHSFDISGAHDQEQAFAAAEAELKS
                     IQARITDGADFAKLATELSEDEGSKEKGGDLGLFGHGQMVKPFEEAAFAAKPGSLAGP
                     VRTQFGWHLIKVEEKVAARTLSIDEARAEIISAIRQEQSTQKAVSDLELCVELLKQGT
                     RTFAQLAEKFSTGESKKNGGKLPIFWKGEFTEDYTDADRRILEAEIGRGNRIDPQIEE
                     LLFQMKTKDLSSVVKTDNGYHLFQVDTVLDPAQLSFTPSLKTRIRRLLEEKKAKELAM
                     AEAKKMAKELGLAEVGQNTNETSAANFAELTKKTIGEEPVKMGPLPFSLAPGFSSYGL
                     TGAIGQVSADGRTYLPPIQKALSDFVNKHRQKFTGSTVPASGTSPEAGKSLPATIDPK
                     EAILGPIESDLGVHFLKLTYVGIDMYEPFAEIKEDLRAMLTQIPREEEVKSQFEKNRE
                     RFDKPATRKVRQMVIADEASARKAYDELKSGQLFTLVSRSYSLDGGSQDARGVVNEIK
                     KGQLPANIDEKVWELKKGEYTEPLQTSYGWVITQVEEIAEATPAKYETVAEQIRGSMK
                     NQLRNQLAQRFINGLRQQARIVRNAELLSEL"
BASE COUNT         1028 a         1432 c         1600 g         1142 t
ORIGIN      
        1 aagagagcca aagcgctgag gcggacgacc agggttttca tgaattctcc tgagtgcaag
       61 atgaatcgat accgtggctc attgggaacg aacctgcgga tcggatgggg aatcgatggg
      121 gattcggacg gaaaaggccc gacccgttct gaacgggccc gaggtgtgtg tgccgaccgg
      181 aatgatcagg ccctcgaggt gagttgctga cgacgcgaac ccttgttcga cgagaagttg
      241 ggccggttgt gtggcggttt cgctctgaaa ccgttgaggt gcggtgtcac ggatgacccc
      301 ggcttgcagt ggaagaaact ggatcggctc tgtcagaggg ccatcaggtg ttggttcgat
      361 cgtgatgagc cagagccgat cgctagtgga gccggcctgc agtcgcaagg tcgattgggc
      421 gtgaggcagt cggagacgcg cttctgaaaa aaccgtcacg agtgccgggt cgagaacaga
      481 cggctgtgtg gagacggcaa tcgtcgattc gagagaggcg gaaaagggcc tgcactcctc
      541 gccgcacagc agtgcttgca cctcgagacg cagcgggaag ctggttacac ccgtaacaga
      601 agcgggaatg gcgacctcga aggggaacac gacggttccc tgatagaggt acaaaacctc
      661 accgtgctgc tcgtggcggg ttggaacggg ccattgaatt tcgccaaccc gataccccgg
      721 tggaagagta agcgtaaaag aaggtggcat gccggaatca cccggattca accagtacaa
      781 atgcaggtcc gccggcagag tgacctgcag ggcgaacgtg gcagttgccc cgggaatggc
      841 ctgcatcatc tcggaaagca tctggagccg aaccccatct acttcgatgg aaggggcggc
      901 agaagcggcg atcagtcgtg ccggcctttg tgccagaacg aaaatcgcga agagaaaaca
      961 ggttgcgaag ctgatggaaa gtcgaaccga aggtcggcga aagaagccat tcggcgagtc
     1021 tgggctcgcc gcgctttcga cgacatgaaa tccctgtcgc atgtcgccag tatagcggtc
     1081 ctcagtcggt cgggcaacag ggaaccggaa gcgtgcccgt ggagcgcatt tctcgctgac
     1141 gactgggcaa cacacgcgca tcaggcaagg ttgtctgcca gggcaagata ggtttcgtga
     1201 gagagatttt ccgcacgaac gccggcgtcg agtctgagcg tggcgaagac gtcttcccac
     1261 gggcgatgga tgacggtgtg tcgcttcttc aacagattca cggtcatctt gcggcgttgc
     1321 tcgaatgccg ttgatgccag agacaatgcc cgctgtcgtc gctggcgttc ctcggtggtg
     1381 aggggcaatg gttcgagcag cagcaggctg ctccagacct gtgggttggg aaagaaggag
     1441 gcgggcgaga aggtagcccc gaccctggaa tgcgcgtaca acccgacggc gacagaaaga
     1501 aaactgtagt catcagatcc ggggcgagca caggctttgc gggtgacttc gcgttgcagc
     1561 agaaaaacac cgcgtcggaa gcgcgccggc ggtgcctcga gcagtcgtct gaccatggcg
     1621 gtaccggaag agtaggggag attgccgcaa atgatcaggg ttccagacgt tgggaccgct
     1681 ggtgtgtgat cgacttcgag aaaatcgccc cagatgacgt gagtctgtga aaatggcgca
     1741 agcgtctcgt gcagatgagg acgcagacgt tcatccattt caaagcacca gagcggattt
     1801 ccctgtttgg ccagacgacg ggtcagggcg cccaggccgg ggccgatctc ccagatcgtg
     1861 tcgtccggct gcgtctgcag agcctcgacc accgactcgg acaaccgttt gtcgacacag
     1921 aagttctgcc cgagggactt tctcgggtcg atgccgagtt gctgcagcag gtcgcgaatc
     1981 gattgcatca gtgctggtcc accgggtcgt cctcccacca ggactcatcc tgcgttggct
     2041 tgcggggttc atcacgcagg gtctcgagat acttcggccg gtcgctcttc cgcatcgttc
     2101 cgctggtcag ttcctgcaga actcgtactg atacttcacg aaacggcaga tgcaggtcga
     2161 tgacgtcgcc agatttgact tcgcgggttg gcttggctcg cactccattc acattcacca
     2221 gaccgcgatc acaggcctca tttccaagca cgcggcgctt gatgagaccg ctgacctgaa
     2281 gaaacttgtc cagtcgcatg cgtgatcctt tcaaaaaaaa agcgccttct tcgagggtgt
     2341 tcctcgaaga aggcgcggga acaactagag ttcgctcaac agttcagcgt tgcgcacgat
     2401 gcgtgcctgt tgccgcaagc cgttgatgaa gcgctgtgcc agctgattgc gcagttgatt
     2461 cttcatcgag ccgcgaatct gctcggcgac agtctcatac ttggccggcg tggcttccgc
     2521 gatttcctcg acctgcgtaa tgacccatcc gtagctggtc tgcaggggtt cagtgtattc
     2581 acccttcttg agttcccaga ctttttcgtc gatgttggcc ggcagctgac ccttcttgat
     2641 ttcgttgacg acgcccctgg catcctgcga tccgccatcc agagagtagg aacgggaaac
     2701 caaagtgaac aactgacctg acttcaattc gtcatacgct ttgcgcgccg aagcctcgtc
     2761 ggcgatgacc atctggcgca cttttcgcgt cgcgggcttg tcgaagcgct cacggttctt
     2821 ctcgaactgg gacttcactt cttcttcacg gggaatctgc gtcagcatgg cgcgcaggtc
     2881 ctccttgatt tcggcgaacg gttcgtacat gtcgatgcca acgtaggtca gcttcaggaa
     2941 gtgaactccg agatccgatt cgatcggtcc cagaatggct tccttgggat cgatggtggc
     3001 aggcagtgat ttgccggctt cgggagaggt tcctgatgcc gggactgtgc tgccggtgaa
     3061 cttctggcga tgcttgttca cgaaatccga caacgctttc tggatcgggg gcagataggt
     3121 gcgcccgtcg gcagaaacct gtccgatggc cccggtcaga ccataactcg agaacccggg
     3181 tgccagagag aacggcagcg gccccatttt caccggctct tcgccgatgg tcttcttcgt
     3241 cagttcggca aaattggccg cgctggtttc gttggtgttc tgtccgactt cggccagacc
     3301 aagctcttta gccatcttct tggcttcggc catggccagt tccttggcct tcttttcctc
     3361 gagcaggcgg cgaatgcggg tcttcaatga cggcgtaaac gacaactgag ccgggtcgag
     3421 cacggtatcg acctggaaca gatgataacc gttatcggtc tttaccaccg aggagagatc
     3481 cttggtcttc atctggaaca acagttcttc aatctggggg tcgatgcggt tgccacgccc
     3541 gatctcggct tcgagaatcc ggcggtcggc atcggtgtag tcttcggtga actcgccctt
     3601 ccagaagatc ggcagcttgc caccgttctt cttcgattcg ccggtcgaga acttttcggc
     3661 cagttgagcg aaggtgcggg ttccctgctt gagcaactcg acgcacagtt caagatcgga
     3721 aacggctttc tgggtcgact gctcctggcg aatggccgag atgatctcgg cacgggcttc
     3781 atcgatgctc agcgtgcgcg cggcgacctt ttcttcaacc ttgatcaggt gccagccgaa
     3841 ctgcgtgcgt accgggccgg cgaggctgcc gggtttggcg gcaaaggcgg cttcttcgaa
     3901 aggcttcacc atctgaccat ggccgaacag gcccaggtcg ccgccttttt ccttcgatcc
     3961 ttcatcttca gaaagttccg tggccagttt ggcaaagtcg gctccgtcag tgatgcgcgc
     4021 ctgaatagac ttcagctcgg cctctgcggc cgcgaaagcc tgttcctgat cgtgcgcgcc
     4081 cgagatatca aagctgtgct ggctctgtcc gtcacgaatc tcgagtttgg ccggcagagc
     4141 gtctttcggc agctgaatgc agacttcacc gtacacgttg ccactgctcg gggtgccgga
     4201 ttgcgggaat tcgagcggtg acccggcttt gtcctgcgat tccttgtcgg gggagtagcg
     4261 cccgccgtcc ttggttacca gaacgatatc cttgaagttc aggttgatgc cgctcttgcg
     4321 atcgctgatg cccaattcga aggtatattc agatttctgg ccttcggtag ctgggacaaa
     4381 acggaaatcg cgcaggttcg cggtgaaggt gtcgagtttc ttttcgaacg tgttgcgggg
     4441 cttgatgaga atgtgtcggg cccgaacctg ttctgatttc ttgaatttgt ccaggttccc
     4501 cgtgtaatac tggcggatcg ccggctcttc cggggtgaac tgtttctgat actcgggatc
     4561 gtcaggattg atgaagacgt gcagcacgga aagacgcttc gaagtctgga actcgttgca
     4621 gtggatcttg tggtattcag caacctcgtc gtcagacacc gttaccttgc tcaggaatcc
     4681 gtggggtgag aagagcacat gttcgaagct gacttcgtca tctttcttga agcgaatgcg
     4741 gttctgggtg tagtaggctt caatggccgc atctgtcgct gccgacgctg tcatcgggcg
     4801 cgtctggctt tcgaagatct cctgcatgac cagcatctgg tgcttgcggg cctgatactc
     4861 ggtcttggtc agcccctgct tctgcagctg gcgactgaat tcggggggcg tcagctgcgc
     4921 ttcttcctgc ttcacgatct gcgacgcggg aacgcggggc tgcacgttgt tgatttcggc
     4981 atagcgctcg agcaacagcg tgtcgacccc caatttctca cgaaaggcca atacgacggg
     5041 gattgaacct ccttcgcgaa tgaaaacggg agcgcggcca aagccggctt cgatcgcccg
     5101 cgccgccgcc gagacgtaag ggctgtcgag cggaaccacc acgccccgcg cggagttcat
     5161 accaattaac tcccactcga tacccggcgg gcaattctgt tc
//
LOCUS       DAORHD010000149         5182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16674_length_5182_cov_3.608933, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000149 DAORHD010000000
VERSION     DAORHD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16674_length_5182_cov_3.608933"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PKO06_04515"
     CDS             <1..924
                     /locus_tag="PKO06_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNV68939.1"
                     /translation="ITVPRIPPMPGLDVVSQRSAQNMQIINGVGNIQVQTVAEILPKA
                     PGKYTIPGFSLKGPDGKIHTTKAIDITVTEEKTNPTEAPSETSTPADETEDRGGISLV
                     RGVFVVLGVILLVIVSPLAISYVLSLSQKRGSSRFATAENDRASHQQSGRSSAAGGGS
                     SASTNSSASSGASREGPASRKPEPSASRPATAAPVTMTRPASIDFDAELLAIKRRNPD
                     GGGDLHRETFDLLHRALVEATGGSLAALTPGELMTDLSRRAPPQTIKVIRRLAEEWEH
                     LVYARMSPARPFAGVEKDVRAILEWARLQEK"
     gene            927..1928
                     /locus_tag="PKO06_04520"
     CDS             927..1928
                     /locus_tag="PKO06_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV68940.1"
                     /translation="MTGTVSPTLQPQLEKAAQTLSGLRTEVGRLLVGQQDLVDRLLIA
                     LVTNGHILLEGVPGLAKTLAIKALARAIEVKFSRIQFTPDLLPADLIGTRIFDPAKTA
                     FFTHKGPVFSQFVLADEINRAPAKVQSALLESMEERQVTIEHETFTLPEPFMVLATQN
                     PIETEGTYSLSEAQVDRFLFKVVVGYPSFEEEREIVRRMGLAEEKHGLDTIKPLIDGH
                     GVMMLRDLARQVYVDDKVVEYILRLVEATRNPEKYGLDLRSYIRFGASPRASIYLTRA
                     ARSLALVKGRPYVKPDDVKAILPDVLRHRVIPTYEAEADEVTSDEIVRRLAEGVPVP"
     gene            1970..2857
                     /locus_tag="PKO06_04525"
     CDS             1970..2857
                     /locus_tag="PKO06_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV68941.1"
                     /translation="MLPKDLLREVRKIEIRTRGLVDSMYAGGYRSVFKGRGIEFSGIR
                     EYSRGDEYRSIDWKVSARSGELHVREHIEERELQVMICLDLSGSMCFGSGSREKRETA
                     VEFGAAMAMAAARNNDRVGICLFTDRVEKKVPPAKGRTHILRLIRDLLYFVPVEKHTD
                     LRSAIEFVRHTLKQKSVVFLVSDFVEGEIPEKELKTLAARHDLIAVLMSDPREEEIPD
                     VGLIELEDPETGCETIFDTGDRALVARLLNRAQKRRDHLLARLRRLGIDTLQLTAGKP
                     VVPAICRLFSEREQRLAHP"
     gene            2869..4113
                     /locus_tag="PKO06_04530"
     CDS             2869..4113
                     /locus_tag="PKO06_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68942.1"
                     /translation="MNQQQQLLQRSNITLLLLIGGLFLGLALTWEQIGYGNQAQARLK
                     ADYAAELLAHGLDREAAEQLEQVLSVAPDDAGSARQRRVLADLYLDRLGDHEKALAHL
                     VHLRRFHPDLSSGTEDLVRRCLDRLGRVYDVQRRHLLDAGINPVKGNVASDTVVKFGA
                     ESGIRLAELQQRLVQLGVPLKSPPPDVLNRIVQGMVSELLLKRAAVRSSVDRDPKYLE
                     QVRAFEQNLLLQRYLEDKVLKDVQVDEQALQLFLEKHQQEFSSPLRVVYSSFSFPDEA
                     QARDYLQDQTRIASRTVVADHVNAVRADLPRPLQGINFDVEKPSGPLGPIEIDGAWHV
                     FVVHDVVPGRQVSPELARQQARLRLLEEKQGGRLTQAIQELARQEEVKILDDVLQASF
                     GLASTTAQTPELKQPPTTGPSR"
     gene            4157..4843
                     /locus_tag="PKO06_04535"
     CDS             4157..4843
                     /locus_tag="PKO06_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68943.1"
                     /translation="MNRCGRSAAVMTAGLLAWWADIACLAATTSASAARAIGSESASV
                     PVASAAGSLPPLPPGEEIYDLLPSRVIPLPWMSLLFDTLIVLILVLAGWILLKKIVNQ
                     GIETEAVVEPLPPDPLEEAIDRLQQVRTHLAAGILNDKDLCEQLALILKVFAWRRVRA
                     GFGGGSTSDELIDGLHREQIEPALIKDIEQVQNLTDAVKFARGTLGALSPVEMIAGYE
                     TLIRRPEWNR"
     gene            4840..>5182
                     /locus_tag="PKO06_04540"
     CDS             4840..>5182
                     /locus_tag="PKO06_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HNV68944.1"
                     /translation="MRWYSPDNVLLLLIVAVALLYRHFVQRSREKGGIRFPLTAQIMR
                     EQPSRKTRLLWLPEALRLGTLVLLVLSLMRPQYGLEQQQVTRKGLDIMLVLDISGSMA
                     AEDFKPNRLKAA"
BASE COUNT         1059 a         1507 c         1569 g         1047 t
ORIGIN      
        1 atcaccgtcc cgcgcattcc ccccatgcct gggctcgatg tcgtgtcgca gcggtcggcc
       61 cagaacatgc agatcatcaa cggggtcggg aatattcaag tgcagacggt tgccgagatt
      121 ctgccgaagg cccccggaaa atacacgatt cccgggttca gtctgaaagg ccccgacggc
      181 aagattcaca ctaccaaggc tatcgatatc acggtgaccg aagagaagac caacccgact
      241 gaggcgccgt cagaaacctc cacgcccgcc gatgaaacgg aagaccgtgg gggcatcagt
      301 ctggtgcgcg gagtatttgt cgttctcgga gtcattctgc tggtcattgt ttcacctctc
      361 gccatctcgt acgtcttgtc tctgagtcag aagcgtggat cttcgcgatt tgcgacagcc
      421 gaaaacgatc gcgcttcaca ccaacagagc gggcgctcgt ctgccgctgg tggcggcagt
      481 tcggcgtcga cgaactcgag tgcttcgtca ggcgcgtcac gtgaaggacc cgcctcgcgg
      541 aaaccggagc cgagtgcatc tcgaccggcg accgcagccc cggtgaccat gacgagacca
      601 gcttccatag actttgacgc tgaattactg gcgatcaagc gccgaaaccc ggatggcggc
      661 ggtgatctgc accgcgaaac cttcgacctt ctgcatcgag cgctggttga agcgacgggc
      721 ggctctctcg cggcgctcac ccccggtgaa ttgatgaccg atctgtcgcg tcgagcgccg
      781 cctcagacaa tcaaagtgat tcgtcgtctt gccgaagaat gggaacacct cgtctacgcg
      841 cgcatgtcac cggctcgacc gtttgctggt gtcgaaaaag atgtcagggc tatcctcgaa
      901 tgggcccgct tacaggagaa atgaccatga ccggaaccgt ctcacccacg ctgcagcctc
      961 aactcgagaa agccgcccag accctgtcgg ggctgcgaac cgaagtcggc cgcctgttgg
     1021 tcggacagca ggatctcgtt gatcgactct tgatcgcgct ggtaacgaac ggccacattc
     1081 tgctcgaggg cgtgcctggt ctggccaaga ccctggcaat caaggcgttg gcccgggcga
     1141 tcgaggtgaa gttctcgcgc attcagttta cacccgacct gctgcctgca gatctgatcg
     1201 gcacgcgcat cttcgaccca gccaagaccg cgttctttac gcacaaaggg ccggtctttt
     1261 cgcagtttgt cctggctgat gaaatcaatc gcgcgcctgc caaagtgcaa agcgctctgc
     1321 tcgaatccat ggaagagcgc caggtcacga tcgaacatga aaccttcacc ctgccggagc
     1381 cgttcatggt actggcgacc cagaatccga tcgagacgga agggacgtat tcgttgtcag
     1441 aagcgcaggt cgatcgcttt ctcttcaagg tcgtggtcgg ctacccgtcg ttcgaagaag
     1501 agcgtgaaat cgttcgccgc atgggcctgg ccgaggaaaa gcacggcctc gataccatca
     1561 aacccttgat cgacggccac ggagtcatga tgctgcgtga tctggcccga caggtatacg
     1621 tcgacgacaa agtggtcgag tatatactgc gtctcgtcga agccacccgc aatcccgaga
     1681 aatatgggct cgatctgcgt tcctacattc gtttcggggc ttcgccgcgt gcctcgatct
     1741 acctgacccg cgcggcgcgc agcctggcac tggttaaggg gcgaccgtat gtgaaacccg
     1801 acgatgtcaa ggcgattctg cccgatgtgc tgcggcaccg cgtcattccg acctacgagg
     1861 ctgaagccga cgaggtgacc agtgacgaga tcgttcgccg cctggctgaa ggagtaccgg
     1921 ttccctgatc gttagaatgg ctattcgagc ctgaaagaga gagcgaaacg tgctaccgaa
     1981 agatctgctg cgtgaggttc ggaagatcga gattcgcacc cggggtctcg tcgattccat
     2041 gtacgcgggc gggtatcgct ctgtgttcaa ggggcgcggt atcgagtttt cgggtattcg
     2101 ggaatacagt cgcggcgacg aatatcgcag catcgactgg aaggtgtcgg ctcgctcggg
     2161 agagctgcac gtgcgagagc acatcgagga acgagagctg caggtcatga tctgcctcga
     2221 cctgtcgggt tcgatgtgtt ttggcagcgg ttcacgcgaa aaacgcgaga cggcggtcga
     2281 attcggcgcc gccatggcca tggccgcggc tcgtaacaac gatcgggtcg ggatctgcct
     2341 gtttaccgat cgagtcgaaa agaaggtgcc accggcgaaa ggacgcacgc acattctgcg
     2401 tctgatccgt gatctactct acttcgtgcc tgtcgagaaa cataccgatc tgcgttcggc
     2461 gattgagttt gtccgacata cgctgaaaca aaagtctgtt gtgttcctgg tttcagactt
     2521 tgtcgaagga gagattcccg agaaggaact gaaaacgctc gccgctcgac atgatctgat
     2581 cgccgttctg atgtcggacc cgcgcgaaga ggagatcccc gatgtcgggc tgatcgagct
     2641 cgaagaccct gagacgggct gtgaaacgat ctttgatacc ggcgaccggg ctctggtcgc
     2701 tcgattactg aaccgggcgc agaagcggcg cgatcatctg ctggcgcgcc tgcgtcgtct
     2761 cggcatcgat accctgcagc tgacggccgg aaaacccgtg gttccggcta tctgtcgcct
     2821 attttcggag cgcgagcagc gactcgcgca cccctgacgg aggacacgat gaaccagcaa
     2881 cagcaacttc tccagcgcag caacatcacg ctgctgctgc tgatcggcgg tctgtttctc
     2941 gggttggccc tgacctggga acaaatcggc tacggcaatc aggcgcaggc gcgcctcaag
     3001 gcagactatg cggccgagct tctggcgcat gggctcgatc gcgaggcggc cgaacaactc
     3061 gaacaggtgc tgtcggtggc cccggatgac gcgggcagtg cccgacagcg gcgagtgctg
     3121 gccgatctgt atcttgaccg actgggtgat cacgagaaag ctctggcgca tctggtgcac
     3181 ctgcgacggt tccaccccga tctgtcgagt ggcaccgagg atctcgttcg acgttgcctc
     3241 gaccgactcg gacgagtcta cgacgtgcag cgtcgtcatc tgctcgatgc cgggatcaac
     3301 cccgtcaagg gaaatgtcgc ctcggacacg gtggtcaaat tcggtgccga atccggtatc
     3361 cgcctggcgg aactgcagca acgactcgta cagttggggg tgccgctcaa gtcgccgccg
     3421 cccgacgttc tcaatcgcat cgtccaggga atggtcagcg agctgttact gaaacgtgct
     3481 gccgtgagat cttcagttga ccgagatccg aagtatcttg aacaggtacg agcgtttgaa
     3541 cagaacttgt tgctgcagcg gtatcttgaa gacaaggtgc tgaaagatgt gcaggtcgac
     3601 gagcaggcgc tgcaactgtt tctcgaaaag catcagcagg agttttcctc gccgctgcgc
     3661 gtggtctact cgagtttctc ctttcctgac gaagcacagg ccagggacta tctgcaggat
     3721 cagacccgca tcgcttcacg aaccgtggtg gcagaccacg tgaatgcggt gcgcgccgat
     3781 ctgccgcggc cgctgcaggg tatcaatttc gatgtcgaga aaccgtctgg tccgctgggg
     3841 ccgattgaga tcgacggtgc ctggcatgtt ttcgtggtgc atgacgtcgt tcccggccgt
     3901 caggtgtcgc ccgagttggc acgccaacag gccagactgc ggctgttgga agagaaacag
     3961 ggagggcgtc tgacccaggc gatccaggaa ctggcccgtc aggaagaagt gaagattctt
     4021 gatgacgtgt tgcaggcgag cttcggactc gcctcgacca ccgctcagac accggagctg
     4081 aagcagccac cgaccaccgg gccctcgcgc tgaagcgtgc gagcctctcg accaattcac
     4141 cggagatcct gattccatga atcgttgcgg ccgatctgca gcagtgatga ccgcaggcct
     4201 tctggcctgg tgggcagaca tcgcctgcct ggccgcgaca acgagcgcct ccgccgctcg
     4261 tgccatcggg tccgaatcag cttccgtacc ggtcgcttcc gcagccggct ctcttccgcc
     4321 gctccctcca ggagaagaga tttacgatct gttaccgtcg cgggtaatcc cgctgccctg
     4381 gatgtctctc ctgttcgaca ccctgatagt cctgattctg gtgttggccg gttggattct
     4441 gttgaagaaa atcgtcaatc aaggaattga gaccgaggct gtggtcgagc ctctgcctcc
     4501 cgacccgctc gaagaggcga tagaccggtt acagcaggtt cggacacatc tggcggccgg
     4561 aattctgaac gacaaggact tgtgcgagca attggccctg attctgaaag tctttgcctg
     4621 gcgtcgagtg cgagccgggt ttggcggggg cagcaccagc gacgaactga tcgatggact
     4681 tcaccgcgag cagattgagc cggcgttgat caaagacatt gagcaagtgc agaacctgac
     4741 cgatgcggtg aaatttgcgc ggggaactct gggcgcactg tctccagtcg agatgattgc
     4801 cgggtatgaa accctgatac gacgaccgga gtggaaccga tgagatggta ctctcctgac
     4861 aacgtactgt tgctgctcat cgtcgcggtg gcgctgctgt atcgtcattt cgtgcagcga
     4921 tcgcgcgaaa aaggcggcat ccggtttccg ctgaccgcac agatcatgcg tgaacagccg
     4981 tcgagaaaaa cccggctgtt gtggctcccc gaagcattgc gcctgggaac tctggtgctg
     5041 ctggtgcttt cacttatgcg tccgcagtat ggtctcgagc agcagcaggt aacgcgcaaa
     5101 ggtctcgata tcatgctggt cctcgatatt tcaggaagta tggccgctga agacttcaag
     5161 ccgaatcgcc tgaaggccgc ac
//
LOCUS       DAORHD010000150         5179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16688_length_5179_cov_3.824356, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000150 DAORHD010000000
VERSION     DAORHD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16688_length_5179_cov_3.824356"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKO06_04545"
     CDS             complement(<1..842)
                     /locus_tag="PKO06_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68945.1"
                     /translation="MSETSLFEDLRSADPAVRYSVLSRIENRPWTPAQVAEFRDLVRS
                     EADVDTRFHMELVLRLKDRSQEASARATLADVEAVLSVTPTDYFKLALLLQHLDPAHA
                     PLVVDLLREKQWHEFPGEVLPFVLRLMKKHGTPQDLPAIETLCRHSHPRVLQAAVEAL
                     EKLNPDDLKSFIVPLLVNASSGIQSLAIRLLHRWDAAAARAHFEAMLFDEERAVRETA
                     LVHAYFFPFAEIEELMLRFLGVEQDDELLRKASRLFIINPTFATVERLSELYETATKS
                     RKEL"
     gene            complement(954..1598)
                     /locus_tag="PKO06_04550"
     CDS             complement(954..1598)
                     /locus_tag="PKO06_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNV68946.1"
                     /translation="MELPSRWIVMLVILSLLSLFFFPSTSYADANWMKYFRKDSLLNT
                     YMRAYAVDGERLWVGTWGDGVVVYTGKDTISYNNRNTRSTPTNNDGLLSDMITSLSID
                     SKNRRVWIGTNEGLASCNLDGADWKSFTSRDGLPNDVIRDVCADEKGNVWVGTPSGVA
                     LFDGQSFKTFDSSSGLYADSVHSITVKGDSVWVATVDGSVNRFDGSAWQVMIHR"
     gene            1777..2598
                     /locus_tag="PKO06_04555"
     CDS             1777..2598
                     /locus_tag="PKO06_04555"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNV68947.1"
                     /translation="MKRLCVVTLLLLGVCGFAFATSMIPKEVRIDDMSNGKDLTVYVG
                     QTLSISLAENPTTGYRWTVEDYKPALLEQLETIFKASGPTTPGSGGTRIFGFLAKKAG
                     ETALALEYQRPWSETAPPAKTFSVKLKIVKAGSMPDVSKPRPGPGMNLGKTLSELSAE
                     LGKLKGKELDQTSVVETRLQNGNKEYVSLVVVTDGRLPRCPHEKLPVTNRALATMVKS
                     LLGHYGKNAAGKELAFLLGGLPQPGYVQVTLTGTGLGLYFNIETGEPMGKLTGKL"
     gene            complement(2768..3532)
                     /locus_tag="PKO06_04560"
     CDS             complement(2768..3532)
                     /locus_tag="PKO06_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68948.1"
                     /translation="MRRLVLSLMLMLVLQPLSGESIDQRMLAQKGMLLAEIGDDQILC
                     VINGNMDEPAARWFLLAGHWRNRELASAATAFLQVTELAPSPDKRLLAVTSVAEGATM
                     LEIFDLAHFLRQEEWKLMCDCNPFPGQIQINCWRKGYLEVETDFPLYLNTPRLLNRDL
                     LRLDHETTFLIDPETGTVMGGASMREKYLRGVLADLRSPDVNRRTEAVRKIGALNEPE
                     LTKALTTALKRERSKGLRKQMRDTLFDLAQVNPDLK"
     gene            complement(3646..4143)
                     /locus_tag="PKO06_04565"
     CDS             complement(3646..4143)
                     /locus_tag="PKO06_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68949.1"
                     /translation="MKNRLSMLMLAVFVLVGCSTMAQATTWEFTKAKVEITYPDTWTH
                     EVNKQEVDVIGSPDGNAAAIIRVVTAKDLEKALEETIEGLAREFGELEDKEPKTEELK
                     INGMDAMFLDEIRTVDKKTEIGIAMIVTPVGQIVTLAVIGTPEAAKKYEKDFGELIKS
                     IKPKQ"
     gene            complement(4320..4451)
                     /locus_tag="PKO06_04570"
     CDS             complement(4320..4451)
                     /locus_tag="PKO06_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68950.1"
                     /translation="MQHPRSGRCACVSVLLAMLLLPVIAAHPLLGFVVLVWLLSSGD"
     gene            complement(4451..>5179)
                     /locus_tag="PKO06_04575"
     CDS             complement(4451..>5179)
                     /locus_tag="PKO06_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68951.1"
                     /translation="AWDPGSGKGDRSGDPPPASIIVTNGGILVKRCWTTLGVALFLAM
                     TLTLSALDCGTCGRKGLPALAMTCPQCRSLLYQPTNRVKAKKSAVLVVEIFFTGNKPE
                     KLPEYAKLYINRKYVGNIPQTEREARGQSLATVGRTGLGFDYTAKYVTELREQHIGLV
                     QVEVEMRFKRLFGVSRSHRRVLFRNVNLKADEKTFLRHYFDHPTTFSKVKPVPPPQDH
                     KPGSSYVPEVKTGTGTLSLETPLF"
BASE COUNT         1133 a         1511 c         1444 g         1091 t
ORIGIN      
        1 aaaagctctt ttctgctctt ggttgccgtc tcatacagct cggacaaacg ctcgaccgtt
       61 gcgaaggtcg ggttgatgat gaacaggcgg ctggctttgc gcagcagttc gtcatcctgc
      121 tcgacgccaa gaaaacgcag catcaattct tcgatctcgg caaacggaaa gaagtaggca
      181 tgaacgagcg ctgtctcacg cacggcccgc tcctcatcga acagcatcgc ttcgaagtgc
      241 gcccgagccg cggcggcatc ccagcggtgc agcagacgaa tggccagaga ctgaattccg
      301 gaactggcat tgaccagcag cggcacgata aacgacttga ggtcatccgg gttgagtttc
      361 tcgagagctt cgaccgcggc ctgcaacact cgcgggtgcg aatgccggca cagcgtctca
      421 atggcgggaa gatcctgggg agtcccgtgt tttttcatca gacgcaggac aaagggcagc
      481 acttcaccgg gaaactcgtg ccactgcttt tcccgcaaca gatcaaccac aagcggggcg
      541 tgcgccggat cgagatgttg cagcagaagc gcaagtttga agtagtctgt tggcgtgacg
      601 gacaaaacag cttcaacgtc agccagggtg gcacgcgccg aagcctcttg cgaccgatcc
      661 ttcagacgaa ggaccagctc catgtggaag cgggtgtcga catcggcttc cgaacgcacc
      721 agatcgcgaa actccgcaac ctgagccggt gtccaggggc ggttctcgat acgcgacagc
      781 accgagtagc gaaccgctgg gtcagcagat cgcaggtctt caaacagact ggtttcactc
      841 atgcatcgtg cgctttcttc ggcagcatac ccgtactgtt cgggtgggtc aacgaacggc
      901 cgaaaaagcg aacacccgcc cgaaggcggg tgttcgctcg tggggaacct gtctcagcgg
      961 tgaatcatga cctgccaggc actgccatca aaacgattga cactgccatc gaccgtggcc
     1021 acccagacgg aatcaccttt caccgtaatc gagtgcacgc tgtcggcata caggcccgac
     1081 gaactgtcaa acgtcttgaa gctctgcccg tcaaacaggg ccacaccgct gggcgttccg
     1141 acccagacat tgcccttttc gtcggcgcag acatcgcgaa tgacatcgtt cggcagacca
     1201 tcgcggctgg taaagctctt ccagtcagcg ccatcgaggt tgcagctcgc aagcccctca
     1261 ttcgtgccga tccagacccg gcggttttta ctgtcgatcg acagactggt aatcatgtcg
     1321 gaaagcagac catcgttgtt ggtcggcgtc gagcgcgtgt tacggttgtt gtagctgatg
     1381 gtgtccttgc cggtgtagac cacgacccca tcaccccagg tgccaaccca gagccgttcg
     1441 ccatcgaccg cataagcgcg catgtaggtg ttgagcagac tatccttgcg aaagtatttc
     1501 atccagttgg catcagcata ggatgtcgac gggaagaaga acagactgag cagactgagg
     1561 atcacgagca taacgatcca gcgtgaggga agttccacgg cagttgcctc ctgaacggta
     1621 cgatatctat ttcactcacc atcatcgaca acttcctgcg atttcctgag ctttgacagg
     1681 acagatcgac gatgagtata gtgaacctga gcctcactga gacatcagaa cttctggtgt
     1741 ttccggctca agatgaactt taagaggagg aaaagcgtga agagactgtg tgttgtgact
     1801 ctgcttctgc tcggtgtttg tgggtttgcg ttcgcgacca gcatgattcc caaggaagta
     1861 cggatcgacg acatgtcgaa cggcaaagac ctcacggtgt atgtcggaca gactctgtcc
     1921 atcagtctgg ctgagaatcc cacgaccggc tatcgctgga cggtggaaga ctacaagccc
     1981 gccctgcttg aacagctgga aaccatcttc aaggcaagcg gcccgacaac ccccggatcg
     2041 ggtggtaccc ggattttcgg cttcctggca aagaaagccg gtgaaacagc tcttgcactc
     2101 gagtatcagc gcccgtggtc tgaaaccgct cctcccgcca agactttttc ggtgaaactc
     2161 aagatcgtca aagcaggatc aatgcctgat gtgtcgaaac cccgccccgg acccgggatg
     2221 aatctgggga agacgctctc tgagctgtct gccgaactcg ggaagctgaa agggaaagaa
     2281 ctggaccaga cttccgtggt cgagacccgc ctgcagaatg gcaacaagga atatgtcagt
     2341 ctggtcgtcg tcaccgacgg tcgtctgcct cgctgcccgc atgaaaagct tcccgtgacc
     2401 aatagagcgc tggcaaccat ggtgaagagc ctgctcggtc actacgggaa gaatgctgcc
     2461 ggcaaagaac tggcgtttct tctcggcggg ctcccgcaac ctggatacgt gcaggtgacg
     2521 ctgaccggaa ccggtcttgg attgtacttc aacatcgaaa ccggcgaacc gatggggaaa
     2581 ctgaccggca agctgtaatt tctggttgtc gaaaaacctc cgacccaatc aagggttcgg
     2641 aggttttttt cttgtgagat cacggctctg gacgctgagg ctgaacctgc cagactcagt
     2701 ctgcggtgtg tgactcaacc tgtcgcaccg agcgaatccg atcgtgtttc ggcgaccctt
     2761 gaaccggtta cttcagatcg ggattaacct gagccagatc aaacagcgtg tcgcgcatct
     2821 gcttcctcag gcccttcgag cgttctcgtt tcagagccgt ggtcagggcc ttcgtcagct
     2881 cgggctcgtt cagggctccg atctttctca cggcttcggt gcgccggttc acatcgggag
     2941 accgcaggtc tgccagaacc ccacgcagat acttctcgcg catgctcgct ccgcccatga
     3001 ccgttccggt ctccggatca atcagaaacg tggtttcgtg atcgagccgc aacaggtctc
     3061 ggttgagaag gcggggcgtg ttgagataca gagggaagtc ggtctcgact tccaagtagc
     3121 ccttccgcca gcaattgatc tgaatctgcc cgggaaaggg attacagtcg cacatcagct
     3181 tccactcttc ctggcgcaga aaatgggcca gatcgaagat ctcgagcatc gtcgcgcctt
     3241 cggccacgga agtcaccgcc agcagtcgct tgtccggaga aggtgccagt tccgtcacct
     3301 gcagaaatgc cgttgccgct gacgccagtt cgcgattccg ccagtggccg gccagcagaa
     3361 accagcgcgc ggctggttca tccatgttgc cattgatcac gcacagaatc tggtcatcac
     3421 caatctccgc cagcaacatt cctttctgag ccagcatccg ctgatcgata ctctcgcccg
     3481 acaagggttg caataccaac atgagcatga ggctcaacac gagtctgcgc attccatctt
     3541 ctcctgtttt cgtacatgtt aagggcggcc tcaaccgaga gaccgccctg agtttcagca
     3601 caacgtcatg aacttttcac agcgaaccct gacactcgaa aatcgctact gtttcggctt
     3661 gatgctcttg atgagttcgc cgaagtcttt ctcatatttt ttcgccgctt caggagtgcc
     3721 gatgacggca agcgtcacga tctgaccgac cggagtcacg atcatcgcaa ttccgatctc
     3781 cgttttcttg tcgacagtcc gaatctcgtc cagaaacatt gcgtccatgc cgttgatctt
     3841 gagttcttcg gtcttgggct ctttgtcttc gagctcaccg aactcgcgcg ccaggccttc
     3901 gatcgtctct tccagagctt tctcgaggtc tttcgcggtg acgacgcgaa taatggcggc
     3961 ggcattgccg tcgggtgagc cgatcacatc gacttcctgc ttgttcactt catgcgtcca
     4021 ggtatcgggg taggtgatct ccaccttggc cttggtgaac tcccaggtcg tcgcctgcgc
     4081 catggtcgag caacccacca gcacgaaaac cgccagcatc aacattgaca atctgttttt
     4141 catacaaccc tcggttttga agttcgcatg agacgccaac accgcctcac gatgcttcat
     4201 tctagccgct gaccggcgaa atagcaaaaa acgagcgtca cagcgcatct ggcgagagcc
     4261 tgggaacact tgggatgagc atcgcgtcac tgagattggg ccgcccgcaa gggcggccgc
     4321 tagtctcctg atgacagcag ccagacgagg acgacgaagc cgagcagagg gtgggcggcg
     4381 atgaccggca gcaggagcat agccagaaga acactgacac aggcacaacg accgcttcgt
     4441 gggtgttgca tcagaacaac ggtgtctcga gagagagcgt tccggtgccg gttttgacct
     4501 cgggaacata ggacgatccg ggcttgtggt cctggggagg cggcacgggt ttgaccttgg
     4561 aaaaggtggt cggatgatcg aagtagtgac gcaggaaggt cttttcgtca gctttcagat
     4621 tcacgttgcg aaacagcacg cgccgatggc tgcgcgaaac cccgaacaac cgtttgaagc
     4681 gcatttcgac ctcgacctgc accaggccga tatgctgttc gcgcagttca gtaacatatt
     4741 tcgccgtgta atcgaacccg agcccggtgc ggcccacagt cgccagtgac tgcccgcgtg
     4801 cctctcgctc ggtctgtggt atgttaccca cgtatttgcg attgatgtag agcttcgcat
     4861 attctggcag cttctcgggc ttgttgccgg taaagaagat ctcaaccacc agcaccgcgc
     4921 tctttttggc cttcacgcga tttgtcggct ggtacagaag cgatcgacat tgcgggcagg
     4981 tcatggccag cgccggcaag cccttgcgac cgcaggttcc gcagtcgagt gccgagagcg
     5041 tcagcgtcat cgccagaaac agcgcgaccc ccagagtcgt ccagcaacgt ttcacgagga
     5101 tacctccgtt tgtcacgatg atcgacgcag gcggcgggtc accagaccga tcgccttttc
     5161 cgcttccggg atcccaagc
//
LOCUS       DAORHD010000151         5175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16715_length_5175_cov_3.897070, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000151 DAORHD010000000
VERSION     DAORHD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16715_length_5175_cov_3.897070"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKO06_04580"
     CDS             <1..587
                     /locus_tag="PKO06_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68952.1"
                     /translation="SRLLVLFSLIDLFDGKVKEAETRLKKAVKITDDPALANMARKIL
                     DDPSLGRGPEARSRLASFLLLPGAPGAAPESLQALGESYLQRGSTFSALKVFIELKDL
                     AEIGRTYLSMASQQFAAGEDKAAAITAGYGTNALNEELRLNPTSAKSHLYLALYYSQR
                     KDRNSCLRHVQAGLKCPMDAQTKRQLLSLRSAST"
     gene            716..1111
                     /locus_tag="PKO06_04585"
     CDS             716..1111
                     /locus_tag="PKO06_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV68953.1"
                     /translation="MAKTVLVAEDSLMLRKVACFPLEKAGYKVLEANNGIEAIEAMSK
                     NEVDLIITDLNMPGMDGFELITQVKDNEKFKHIPIIILTTEGKKDMVMKGLTLGANSF
                     LQKPIKPDLMLQEVERLLAKKGTPGKETK"
     gene            1111..4215
                     /locus_tag="PKO06_04590"
     CDS             1111..4215
                     /locus_tag="PKO06_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68954.1"
                     /translation="MSTNTAALLEKLHDIDPAIRRKAVRDLTSFPEKEVVEALAEALG
                     DPNKGVQNSAIEALTQMRHTSVIEGLLPIIRGSDLNTRNAGMSILKSFGTMALPRLTE
                     ALNKAADVDEIIQILVVMGNIGSSLATDAILGFVGHDDDNVKTTAVEALGKIQDPKAI
                     KSLINAYHQTDILKYALLEAMGNISVSDAKPLILNAIKSEDVLECFSGIGAMGAMEEP
                     EFLQPLFLKLQSEEDLGTRRLIIKSMAMIEDAVPGSLAKHVDRTAIRPILNILLEQQN
                     SAEFIYIVKVAAALKDEVYAGALLSAFDSSEAEIVDIAFAGLMALAEKGIRPALDRLG
                     RVAPPVAIKILEYIEKFPHADIPKAVVAICQHAEDAVRQVIARTLTANPCEISFNALA
                     KMLQAPDPDEMVRKNAVLGIRKMLQFDGSLSALINALKDANGHVRREAALALGTSSSK
                     QVLEPLMNTLLKEPYGDVREAAAQVLAARKDAEITKKLCELLDSDNSRVRETIAKTIW
                     QCGASGAVDSLIQKLSDKEWRVVVNACQSLEKMKDMKSIFPLKELLKHDDSQIRIAAL
                     SALRAFHSKELKQFLMPLIEDKNATVAKLAIEALSELEDKTLDEVFRKFLNHTAWEVR
                     YQIVKALGKIKSQKAVDSLIQIAQTDPNNAVRAKALLALARINDKKAFDAAMKLLGHP
                     DRDLIIAAIKFFLAFNPKETSSLEEKITAIFQANPWVKNYFLISYPENTCDLLNKVLT
                     ATATPRELRRIERLKEVKPTGQGMTIEETVLLREIVAEKCGIWLADGKALERKLSRDL
                     SKFYINTWMEYYHALRYGGDEQNMTLSLYDIITDPSTGFFGEAEQNKVLVSTIIPEMI
                     AERMKEGRREIRILAAGCSFGPEPYSLAMQILEDVHSDSVKISVVGVDISHICLNTAK
                     RGIYKREMFRHVDQKYVDLYFEDDRGDLRIKDDVKNMVEFKFLNLSCGREMEAIGDFD
                     IVVCRNVFSVFTQREKEKLAESLYNSLVPGGALFIAARESLYNVTKAFKLATYDKVVV
                     YRKM"
     gene            4293..>5175
                     /gene="pseI"
                     /locus_tag="PKO06_04595"
     CDS             4293..>5175
                     /gene="pseI"
                     /locus_tag="PKO06_04595"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HNV68955.1"
                     /translation="MNRSMNLGTRRVGAGHPTYIIAELSANHNQDLEKALALIRAAKD
                     AGADAVKLQTYRPDTLTIDCDKEFFRIKGGLWGGKNLFQLYGEAFTPWEWHQPLLDEA
                     RRVGIDLFSTPFDASAVDFLEQLGMSVYKIASFENVDLPLLRKIASTGKPVIMSTGMA
                     TLAELDESVNVLRQAGCQDLALLKCTSAYPAPASEMHLRTIPHLSAGFNVPVGLSDHT
                     LGIAVPVTAVAVGACIIEKHFTLSRGEPGPDSAFSLEPAEFQAMVTSIRQAEQALGGV
                     HYGLTDKEREGRTFRRSL"
BASE COUNT         1192 a         1336 c         1476 g         1171 t
ORIGIN      
        1 acagccggct gttggtgctg ttttctctga tcgatctgtt tgatggcaag gtgaaagagg
       61 cggaaacccg tctgaagaaa gctgtgaaga tcacagacga cccggcgctg gcgaacatgg
      121 ctcggaagat tctcgatgat ccctcgctgg gccgtggacc tgaggcccgt tcacgtctgg
      181 cttcattcct gctgttacct ggggctccgg gcgcggctcc tgaatctctg caggcgttgg
      241 gtgaaagcta tctgcagcgt ggcagcacgt tctcggccct caaggtgttc attgagctga
      301 aagaccttgc ggagattggt cgtacctatc tgtcgatggc ctcacagcag tttgccgccg
      361 gagaagacaa agccgcagcc ataaccgcgg gttacggtac caacgccctg aatgaagaac
      421 ttcgcttgaa tccgacttct gcgaagtctc acctttacct ggccctgtac tattcgcagc
      481 ggaaagatcg caattcctgt ctcagacatg tgcaggctgg tttaaagtgc cccatggatg
      541 cgcagacaaa acgacaactg ctctcgttga ggagtgcttc gacctgattg tacccgactt
      601 gtcattcgag acctcggaaa tcagctttcc gaggtctttt gttttaggtt gcatcacctc
      661 ctgggagtct atataatctc ctccagtttc ttgaaatctg ctggagggat ctctcatggc
      721 caaaacggtc ttggtggctg aagactcttt gatgttgcgt aaggtagcct gtttcccgct
      781 ggagaaagcc gggtataaag tcctggaggc caacaacggg attgaggcca tcgaagcgat
      841 gtccaagaac gaagttgacc tgattatcac ggacttgaac atgcctggaa tggacggttt
      901 cgaactgatc acccaggtga aggacaacga gaagttcaag cacattccta tcatcattct
      961 taccaccgaa gggaagaagg acatggtgat gaagggcttg acactgggtg ccaattcctt
     1021 tctgcagaag ccgatcaagc ctgatctgat gctgcaggaa gtggaacgcc tgctggccaa
     1081 gaaaggcacc cccggaaagg agaccaagtg atgtcgacga ataccgccgc tttgcttgaa
     1141 aagctccacg atatcgaccc ggcgattcgg cgcaaagccg ttcgcgatct gacttcgttc
     1201 cctgagaaag aagtcgttga agctctggcc gaggctctgg gcgacccgaa caaaggcgtg
     1261 cagaacagcg cgattgaagc gttgacgcag atgcgccaca ccagtgtcat tgaggggctt
     1321 ctgccgatca ttcgtggttc tgacctcaat acccgcaatg cgggcatgtc gattctgaaa
     1381 tcctttggta cgatggcgtt gccacgtctt accgaagcac tcaacaaggc cgccgatgtt
     1441 gatgagatca ttcagattct ggtcgtcatg gggaacatcg ggtcatcgct tgctactgat
     1501 gccattcttg gttttgtcgg tcatgacgac gacaatgtca agaccaccgc ggttgaagcc
     1561 ctgggcaaga ttcaggatcc aaaagccatc aagagcctga tcaatgcgta tcaccagacc
     1621 gacattctga aatacgccct tctcgaagcc atgggaaaca tctcggtttc agatgcaaaa
     1681 ccgctgattc tcaacgcgat caagtctgaa gacgtgctgg aatgcttctc gggcatcggt
     1741 gccatgggtg ccatggaaga acccgaattc ctgcagccgt tgttcctcaa gcttcagtct
     1801 gaagaagatc tgggcacacg ccggctgatc atcaagtcga tggcgatgat cgaagatgcg
     1861 gttccgggca gtctggccaa gcatgttgac cggacagcga ttcgcccgat tctcaatatc
     1921 ctgctggaac agcagaattc cgctgagttt atctacatcg tgaaagtagc cgccgcgttg
     1981 aaagacgaag tctatgccgg cgcactgctc agcgcctttg acagcagcga agcagagata
     2041 gttgacatcg cctttgcggg cctgatggct ctcgcggaga agggcatccg tccggcgctc
     2101 gatcgtctgg gcagggttgc acctccggtg gcgatcaaaa ttctcgaata catcgagaag
     2161 ttcccgcatg ccgatattcc caaggccgtc gtggcgatct gccagcacgc tgaagatgcg
     2221 gtgcgtcagg tgattgcgcg cacgttgacc gcgaatccct gtgagatctc gtttaacgcg
     2281 ctggcgaaaa tgctgcaggc cccagacccc gacgaaatgg tgcgcaagaa cgccgtgctc
     2341 ggcatcagga agatgctgca gttcgacggt tcgctttcgg ctctgatcaa tgcgttgaaa
     2401 gacgccaatg gtcacgtgcg tcgcgaagcg gctctggctc ttggaaccag ttcctcgaag
     2461 caggttctgg agccactgat gaacacgctt ctgaaagaac cttacggcga tgtgcgtgaa
     2521 gccgcggctc aggtgcttgc cgcccgcaag gatgcggaga tcaccaagaa gctgtgcgaa
     2581 ctgctcgaca gtgataattc gcgcgtgcgc gaaacgatcg ccaagaccat ctggcaatgc
     2641 ggtgcttcgg gtgccgttga ttcgttgatc cagaagctgt cggacaaaga gtggcgtgtg
     2701 gtggtgaatg cgtgtcagtc gctcgagaag atgaaagaca tgaagtccat cttcccgctg
     2761 aaagaactgc tcaagcatga tgattcgcag attcgcatcg ctgctctgtc cgcgttgcgg
     2821 gctttccaca gcaaagagtt gaagcagttc ctgatgccgc tgatcgaaga caagaatgcc
     2881 acggttgcca aactggcgat cgaagcgttg agcgagctgg aagacaagac gctcgatgaa
     2941 gtgttccgca agttcctgaa ccataccgcc tgggaagtcc ggtaccagat cgtgaaggct
     3001 ctcggcaaga tcaagagcca gaaagcggtc gattctctca ttcagattgc ccagactgat
     3061 ccgaataatg cggtgcgcgc gaaggcgttg ctggctctgg cccgcatcaa tgacaagaaa
     3121 gccttcgatg ccgccatgaa gctgctgggt caccccgatc gcgatctgat cattgcggcc
     3181 atcaagttct tcctggcgtt caacccaaaa gaaacgtcca gtctcgaaga aaagatcacc
     3241 gcgatcttcc aggcaaatcc gtgggtcaag aattacttcc tgatcagtta ccccgagaat
     3301 acctgcgatc tgctcaataa ggtgctgacc gctactgcca caccgcgcga actgcgtcgc
     3361 atcgagcgtt tgaaggaagt caaaccgacc ggtcagggaa tgacgatcga agaaaccgtg
     3421 ctgctgcgcg agatcgttgc cgagaagtgc ggcatctggc tggccgacgg caaggcgctc
     3481 gaacgcaagt tgagccgcga tctgagcaag ttctacatca acacctggat ggagtactat
     3541 catgcgctcc gctatggtgg tgacgagcag aacatgacgc tcagcctgta cgatatcatc
     3601 accgatccgt cgacgggttt cttcggtgaa gccgaacaga acaaagttct ggtgtcgacg
     3661 atcattcccg aaatgatcgc tgaacgcatg aaagagggtc gacgtgagat tcgcattctc
     3721 gctgccggtt gctcttttgg cccagagcca tactctctgg cgatgcagat tctcgaagac
     3781 gttcactcgg acagcgtgaa aatttcggtg gttggtgtgg atatctcgca catctgtttg
     3841 aacactgcca agcgtgggat ttacaaacgt gagatgttcc gccatgtaga tcagaaatac
     3901 gttgatctgt atttcgaaga tgatcgtggc gatctgcgca tcaaagacga tgtcaagaac
     3961 atggtcgagt tcaagttcct caacctgagc tgtggccgtg aaatggaagc gattggcgac
     4021 ttcgatatcg ttgtctgtcg caatgtcttc tctgtgttta cgcagcggga gaaggaaaaa
     4081 ctcgccgaga gcttgtacaa ttcgctggtt cccggtggcg ctctgttcat tgccgctcgt
     4141 gaatcgctgt ataacgtgac caaggcgttc aagctggcga catacgacaa ggtcgtggtc
     4201 taccgaaaaa tgtgatctga agagatcaaa gggcggtcgg gtttttcccg atcgcccttt
     4261 ttctcttttc gtgagtgagg tatgatgtga tcatgaacag aagcatgaat ctcggaacgc
     4321 ggcgtgttgg agcggggcac ccgacctata tcattgccga gctctcggcg aaccacaatc
     4381 aggatctcga aaaggcgctg gctctcattc gggctgcgaa agatgccggc gcggacgcgg
     4441 tgaagttaca gacctatcgc ccggacactc tgacgatcga ctgcgataaa gagtttttcc
     4501 ggatcaaggg tgggttgtgg ggtggcaaga atctgtttca gttgtatgga gaagccttca
     4561 ctccgtggga gtggcatcaa ccccttctcg atgaggcccg acgcgtcggc atcgatctct
     4621 tttccacgcc gtttgacgca agtgccgtgg attttctcga gcaactgggg atgtcggtgt
     4681 acaagatcgc gtcattcgag aatgtggatt taccattgtt gcgaaagatt gcctcaaccg
     4741 gaaaaccggt gatcatgtcg acgggaatgg cgacgctggc ggaactcgat gaatcagtga
     4801 acgtgttgcg acaggcaggg tgccaagacc tggctttgtt gaaatgtacg agtgcctacc
     4861 cggcaccagc ctcagaaatg catctgcgaa cgattccgca tctgtcggca ggctttaatg
     4921 tcccggttgg tctctctgat cacacgcttg gtatcgcggt tcccgtgacg gctgttgcgg
     4981 ttggcgcctg tatcatcgag aagcatttca ctctgtcacg cggagaaccc ggccctgaca
     5041 gcgccttttc tctcgaaccc gctgagtttc aggcgatggt cacgtcgata cggcaggctg
     5101 agcaggctct tggcggggtg cattacgggt tgacggacaa ggaacgcgaa ggacgaacgt
     5161 tcaggcggtc gttgt
//
LOCUS       DAORHD010000152         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16758_length_5165_cov_3.758904, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000152 DAORHD010000000
VERSION     DAORHD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16758_length_5165_cov_3.758904"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKO06_04600"
     CDS             complement(<1..383)
                     /locus_tag="PKO06_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNV68956.1"
                     /translation="MGKTFIENILARHSKDPIVPGTIIWMDIDFRTARDFGGANVVKN
                     FTSNYPGEKVRDLDRTFFTFDCVAPANNIPYANNQHACRKFARENGLKVFDVDMGIGS
                     HINIEYGQAVPGSTTVGTDSHLNILG"
     gene            606..1553
                     /locus_tag="PKO06_04605"
     CDS             606..1553
                     /locus_tag="PKO06_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV68957.1"
                     /translation="MSFQSGRPDRVRHLQSLSIGAILSVLVLIVTLSHLAFGEERRQF
                     RFACTMQTISKADQEDIRVGMDVWMDLFLKEWQLNYQSKTLFFDSLAQLIPQAKAGAV
                     DLMNITTLQYLQLKQHLDLEPGYLGIVGSSATCSLYILVHSEKPFRSLKDLRDKTILM
                     DSRDHRDLAAMWLDYELIRNQLPPAIGYFGKINTVSKPSEAALPVFFGEADACLIRDR
                     AYRTLVEMNPQVGQDLLIIASSPGLIFDFCSFRAGYADPDKRVIRSSTVKFSDSVKGR
                     QVLTLFRTDRFIPYDEQIIKPTFDLIAEAERLGVKVPVP"
     gene            1575..4214
                     /locus_tag="PKO06_04610"
     CDS             1575..4214
                     /locus_tag="PKO06_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV68958.1"
                     /translation="MLPLPLFRRLFFKVLLLTWLLIVAITGIMFFLVYRTLEATLQRS
                     LTAQAEIIEASISQVAIPAMANRSLSKLVDHCLGVMQVTPQICLITTSDLSGLTLQME
                     PGKWKKRSKPAVWEDQAQTTVGGHIQQNPIGTESIFLYTSELKFGSLPMGWIHIGLSL
                     NSYLSYLDSIKRTAVGIALAGIVIGFLLSIILSRQLTRPLFELEIFSRRVAEGDLTAK
                     VEIRTGDEVERLATSFNLMTVSLARSKQELIASQAFTKSVLQSMADGVAVVSIDGKII
                     EANQALGRLIGVDSLALSGQDFAESLFPDPERFRRDVWSRLQSQNMVEHLQVWVKGAT
                     SPLVPILLSASSVADSGGTIQAVVAIIKDISLLLQAEAQQRRLAVALEQSADAILITN
                     NQGSIDYVNPAFERLYGFTMQEVRGQTPRLLKSGEHSPGFYANLWETIIGGNTWRGRL
                     DNRVKSGAKVTVETSISPIRLPGGPTSGFVALQRDITRQLELEEHLNQSQKVEAIGTL
                     AGGIAHDFNNILCVILASAEVAYDMADGKPELEQIIDGIKLATQRGADLVRQILLFSR
                     RRLGTPEELVSLHTIVKETVKLLRSTIPLTIEIALDLSRENLTIRADPIQIQQLIMNL
                     CTNSAYAMRAHGGRLTITTALIEADTRKTSLFPGTVPGTFVQIKVIDTGTGIPPELLG
                     KVFDPFFTTKPAGEGTGLGLAVSASVVQRYHGGIRIESEVGSGTTVEILLPAITDGEV
                     RDHPTAVQKMRGTGRLVFVDDETTLCRIFEQEFQRLGYDVSVFSNPNAVVTTLRQLTR
                     PPDLLISDYAMPKKYGFDLLRELREHFPDLPVIFWTGNPQAIDPVELERWKVAKVLMK
                     PLSIAALSREIAAQLNAQRTPIS"
     gene            4295..5074
                     /locus_tag="PKO06_04615"
     CDS             4295..5074
                     /locus_tag="PKO06_04615"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNV68959.1"
                     /translation="MMHTIGLILFAVAHGYFAAWLAVWMLFNPREAKFFRGRQLPFTP
                     GLLPGSRCHLELAVADAISAQLLRPDVLEAAAIKQGLPKLIRSTLPEHIETLGSDAQF
                     LETIAACVSGAFKDQLNIRNSAKNKCTDLVPPGHKVPFTEMFSSMFSGKIEEIIDSVC
                     RTRTFVDAMKGAIKHLADDLRRDESELSMKVEQMSGKILGTGLTALNIRGIIHEQLSA
                     LSNEQLEELVHKTAGRHLRDIKNVAALIGIAFGIFSALLFG"
BASE COUNT         1047 a         1503 c         1477 g         1138 t
ORIGIN      
        1 ccgagaatgt tcaggtgact gtcggtgccg acggtggtcg accccggaac cgcctgacca
       61 tactcgatgt tgatgtgcga gccgatgccc atgtcgacgt cgaatacctt gagaccgttt
      121 tcccgggcaa acttgcggca ggcgtgctgg ttgttcgcat acggaatgtt gttcgcgggc
      181 gcgacacagt cgaaggtgaa gaaggtgcgg tcgagatcgc gcactttctc accgggataa
      241 ttggatgtga agttcttgac cacgttagcg ccgccgaagt cgcgggccgt acggaaatcg
      301 atgtccatcc agatgatggt tccggggacg atcgggtctt tcgaatgacg ggccagaatg
      361 ttttcaataa aagtctttcc catgccttcc ctcgtgtgat gatttcggtc agcgcctgac
      421 cgtctgtctc gcaggcgtat ggtactatcg ggcacgcagt cgcgcaaccc cggcgtggcg
      481 ggcaaccggg aacttcattc gcgagtgacg ggcactggaa ctccggttcg tcagacggct
      541 tcacacagcc gaaaaaggtc ttctcatcgc ttgctctgtg agaaggtgca tgataccttg
      601 atttcatgag ctttcaatcg ggaagacctg atcgggtgcg gcacctgcag tcgctgagca
      661 tcggggccat cctgtcggtg ctggtgctga tcgtgacgtt gtcacatctg gctttcggcg
      721 aagagcgtcg ccagtttcgt ttcgcgtgta ccatgcaaac gatcagtaag gccgatcagg
      781 aagatattcg tgtcggcatg gacgtctgga tggatctctt tctcaaagaa tggcagctga
      841 actaccaatc caagacgctc ttctttgaca gcctcgcgca gctgattccg caggccaaag
      901 ccggtgccgt cgacctgatg aacatcacca cgctccagta tctgcagctg aaacagcacc
      961 tggatctcga gcctggatac cttggaatag tcggttcttc cgcaacatgc agcttgtaca
     1021 ttcttgtcca cagtgaaaaa ccgtttcgct ccctgaaaga cctgcgcgac aagacgattc
     1081 tgatggattc tcgcgatcac cgtgatctgg cagccatgtg gctcgattac gaactgatcc
     1141 ggaaccagtt gcccccagcc attgggtatt ttggcaagat caacaccgtc agcaagcctt
     1201 cagaagcggc attgccggtg tttttcggcg aagccgacgc ctgcctgatt cgcgatcgcg
     1261 cctatcgtac gctggtggaa atgaatccgc aggttgggca ggaccttctg atcatcgcca
     1321 gttcgcccgg tctcatcttt gacttctgtt cgtttcgagc cgggtatgcc gatcctgaca
     1381 agcgcgtgat tcgcagctcc acggtgaaat tttcggacag cgtgaaagga aggcaggtgc
     1441 tgacgctgtt tcgtaccgat cgattcattc cctatgatga acagattatc aaaccgacct
     1501 tcgatctgat cgctgaagcc gagcggctcg gcgtgaaggt tccggtgccg tgacaccggc
     1561 ctgctgaaca gttgatgttg cctttacccc tgtttcgccg actgttcttc aaggttcttc
     1621 tgctgacgtg gcttctcatc gtcgccatta ccggcatcat gttttttctg gtctatcgaa
     1681 ccctcgaagc gacgctgcaa cgttcgttga cggcgcaggc cgaaatcatt gaagcgtcca
     1741 tttcccaggt cgccataccg gccatggcca atcgcagcct gagcaaactg gtcgatcact
     1801 gcctgggggt catgcaggtg acgccgcaga tctgcctgat cacgaccagc gatctttcgg
     1861 ggctgaccct gcagatggaa ccagggaagt ggaagaaacg ctcgaaacca gccgtctggg
     1921 aggaccaggc gcagacgacc gtgggtggcc acattcagca gaaccccatc ggtacggaaa
     1981 gcattttcct ctatacgagt gaactcaagt tcggcagttt gcccatgggg tggatccaca
     2041 tcggcctgtc tctgaactcg tatctctcgt atctcgattc gatcaagcgc acggctgtcg
     2101 gcatcgccct ggccggaatc gtcatcggct ttctcctgtc gattattctg tcgcgccagc
     2161 tcacccgacc gctgttcgag ctcgaaatct tctcgcggcg cgtggctgag ggcgacctga
     2221 cggcgaaggt cgagatacgc accggagacg aagtcgagcg tctcgccacc tcgttcaacc
     2281 tcatgacggt ttcgctggcg cgctcaaaac aggagctgat cgcttcgcag gcgttcacga
     2341 aaagtgttct gcagagcatg gctgacggag ttgccgtcgt gtctatcgac ggaaagatca
     2401 tcgaagcgaa ccaggctctc ggccgactca ttggcgttga ttcgctcgcg ctctcgggcc
     2461 aggattttgc cgagagtctg ttcccggatc ccgaaagatt ccgccgtgat gtctggagcc
     2521 ggttgcagag tcagaacatg gtcgaacatc tgcaggtgtg ggtgaaaggt gccacttcgc
     2581 ctctcgttcc gattctgttg tcggcctctt cggtcgccga ctccgggggc accatccagg
     2641 cggtcgtcgc gatcatcaag gacatttctc tgcttctgca ggccgaagcg cagcagcggc
     2701 gtctggccgt ggccctcgag cagagtgcag acgccattct gatcacgaac aatcagggaa
     2761 gcatcgatta cgtgaacccg gcgttcgagc gactgtacgg ctttaccatg caggaagtcc
     2821 gcggacagac accgcgtctt ctcaagagcg gcgagcactc gcccggcttt tatgcgaatc
     2881 tgtgggaaac gatcatcggt ggcaacacct ggcgcggccg cctcgacaat cgcgtgaaat
     2941 cgggagccaa ggtcacggtc gaaacttcga tttcacccat tcggctgccg ggcggtccga
     3001 ccagcggatt cgtggccctg cagcgtgaca tcacccgaca gctggagctc gaggaacatc
     3061 tgaatcagtc gcagaaagtc gaagccatcg gcacgctcgc cggcggaatc gcccatgatt
     3121 tcaacaacat tctctgcgtc attctcgcca gcgcggaggt ggcttatgac atggcggacg
     3181 gaaaacccga gctggaacag atcatcgatg gcatcaagct ggccacgcag cgcggggcag
     3241 atctcgttcg tcagattctg ctcttcagtc ggcgccgtct cggaaccccg gaagaacttg
     3301 tctcactgca cacgatcgtc aaggaaaccg tgaagttgtt gcgttccacc attccgctga
     3361 cgatcgagat cgcgctcgac ctgtcgcgtg agaacctgac gatcagggca gatccgattc
     3421 agattcagca gctgatcatg aatctgtgta ccaactcggc ctatgccatg cgcgctcacg
     3481 gcgggcgttt gacgatcacg accgccttga ttgaagcgga tacgcgcaaa acatcgctgt
     3541 ttcccggtac ggttcctggg actttcgtgc agatcaaagt catcgatacc ggaaccggga
     3601 ttccgcccga actgcttggg aaggtcttcg atcccttctt cacgaccaaa ccggctgggg
     3661 aaggtaccgg tctcggtctg gcggtcagcg ccagtgtcgt tcagcgctat cacggcggca
     3721 ttcgcatcga gagcgaagtc gggtcgggaa cgacggtcga gatcctgctt ccggcgatca
     3781 ccgatggaga agtgcgcgat catccgactg ccgtacagaa aatgcgcgga accggccgtc
     3841 tggtcttcgt tgacgatgag acaaccctgt gccgcatctt tgaacaggag tttcagaggc
     3901 tgggatacga tgtctcggtg ttttccaacc cgaacgccgt ggtgacgacc ctgcgccagt
     3961 tgaccagacc gcccgatctt ctcatttccg attatgccat gccgaaaaag tacgggttcg
     4021 atctcctgcg cgagttgcgg gagcatttcc ctgatctacc ggtcatattc tggaccggaa
     4081 atccgcaggc gatcgatccg gtcgagctgg agcgctggaa ggtggcgaaa gtgctcatga
     4141 aaccgctgtc cattgcggct ctgtcgcgtg agatcgccgc gcagctcaac gctcagcgca
     4201 cgccgatcag ctgattctct tctgccccgg cactcagccc ttccgttcgc tttatgaggg
     4261 cgggggtttg tggtagagtc gcgtggttga aactatgatg cacaccatcg ggctcattct
     4321 cttcgcggtc gcgcacggct acttcgccgc ctggctggcg gtctggatgc tgttcaaccc
     4381 cagggaagcg aagtttttcc gcggccggca gctgcccttc acaccgggtc tgctgcccgg
     4441 gtcccggtgc catctcgaac tggcggtggc cgacgcgatc tccgcacagc tgcttcgccc
     4501 cgacgtactc gaagcggcgg cgatcaaaca ggggttgccg aagctcattc gtagcacgct
     4561 tcccgaacac atcgagacgc tgggcagcga cgcccagttc ctcgaaacca tcgctgcctg
     4621 cgtctccggc gcattcaagg accagctgaa catccgcaac agcgcgaaga acaaatgcac
     4681 cgatctggtt ccgccgggtc acaaggtgcc gttcaccgag atgttctcgt cgatgttcag
     4741 cggcaagatc gaagagatca tcgattcggt ctgccgcacc agaaccttcg tcgatgccat
     4801 gaagggcgcg atcaagcatc tggccgatga cctgcggcgc gacgaatctg aactgtcgat
     4861 gaaggtcgag cagatgtccg gcaagattct cggcacaggg ctgacggccc tgaacattcg
     4921 cggcatcatt cacgagcaac tcagcgccct gtcgaacgaa cagctcgagg aactggtaca
     4981 caaaacggcg ggaagacacc tgcgcgacat caagaacgtc gccgccctca tcggcattgc
     5041 cttcggtatt ttttctgccc tgctgttcgg ctagtatcca agcttctttc cgcggggtac
     5101 ccgtttaaaa cttgtactga tcgtgcaaca cacttttttt taaaaagtct ttgacatgcg
     5161 ggtga
//
LOCUS       DAORHD010000153         5164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16761_length_5164_cov_4.074183, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000153 DAORHD010000000
VERSION     DAORHD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16761_length_5164_cov_4.074183"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..714)
                     /locus_tag="PKO06_04620"
     CDS             complement(112..714)
                     /locus_tag="PKO06_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV68960.1"
                     /translation="MKRRLILTLMVLLLTTGPAFALFCSQCGHNLADDANFCSSCGKA
                     CRDGSSSIDTSPVPTVSPSAPPMVQPVQVVTPIMTTDVAPAYLVTSPYLYLNSQRLDR
                     HHPIRVLEVRGSQARVMFVSNSNPCRPISCWVTIGDLERHTTWRPQFQIVVPGPAPFC
                     FTRSPKRRPIHAPVVVVDHKRKHRHDKHDRHGSTRVIFKL"
     gene            911..2587
                     /gene="ettA"
                     /locus_tag="PKO06_04625"
     CDS             911..2587
                     /gene="ettA"
                     /locus_tag="PKO06_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNV68961.1"
                     /translation="MAEQYIYTMMGLSKFYGQKQVLKEINLCFYPGAKIGIVGENGSG
                     KSTLLRIMAGIDKDFVGHSEPAKGVRVGFVPQEPQLALGKTVRENLEMAVADHMKLLK
                     EYEDISNSMGEMDGDAMDKAMERMGVLQDKIEAAGGWEIDTRLNVAANALVLPPDDMI
                     VDNLSGGEKRRVALCRALLEQPDLLLLDEPTNHLDAETIDWLENYLKDYPGTVIISTH
                     DRYFLDNITKWILELEECRGIPFEGNYTSWLAQKLEILAQGEKKESSRRRSIDRELKW
                     IKMNTADRHELNRNRIAQYEQLVAREKAENKVDTTVIQIAPGEELGNQIVEARNVFKG
                     YGKNELIKDLQFNLPRGAVVGIIGPNGTGKTTLFKMIAGVEKPDKGEVIVGPSVRLAW
                     VEQFRDSLENGKSVYDVISEGKAEIPFGKVSIPARAYCGRFGFKGSDQQKLVENLSGG
                     ERNRVHLAKVLKSGGNLVLLDEPTNDLDVGTLRMLEDAVTDFSGCIMVISHDRFFLNR
                     ICTHLLVFEGDSKVTWFEGNYEEYETKRRKELGAAAVENRRARYRKFAIN"
     gene            complement(2664..3380)
                     /locus_tag="PKO06_04630"
     CDS             complement(2664..3380)
                     /locus_tag="PKO06_04630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV68962.1"
                     /translation="MSRWNPETIAFSLLLTLTFTLFLFAGGAMLEALGLKSLDWVEVA
                     RGKMQSDDPAIRLQGAKLLTTIDDSSLTTLHRSLLNDPSQAVRRAAIEGLRTRQAPHF
                     LDFHLKALSDPVTSLRQLAVENLNHTGTAPWVGEALLKALSDYQPRVQTLALETAVRS
                     YLPKSADRRFAEPLQFLATDPRPAVRSLAVKGLGLLRDPSAQPVLERLAGVDPDPEIR
                     EGAERALTQIARLTPNQHTQ"
     gene            3733..>5164
                     /locus_tag="PKO06_04635"
     CDS             3733..>5164
                     /locus_tag="PKO06_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68963.1"
                     /translation="MAGYLRDIEPDQVLARVLRKADEACDPRWTSDHHFTAQGNEGWS
                     DPGRVVSVVKEAIRRDLGVEPLLMVAVGTGYDTIVIHTDPRAFGDRVAPSQTAWRQWF
                     FHVSEGRKRQGSPVSLSDSRLRAKYATREQWQQLGEFCRKFLQSQFGDYIPEVLINGR
                     PTSFFSPKFGGGRLVIAYRLFPGAPDPSDRFPGGYVLIMRDAEIAREALLRGSAASGR
                     AWRREAVVSEPAFRETPESLDYVHPAPPALFPVFEHGNASPTFLHLIVSVPYNQLIPS
                     LLAWKIPGIVLAVLWLHFGWIFLRRLHDGTLRLNVRIAGQLRFGLLLMVGLSLVALLL
                     FARSYGSLVEQSEQWQRKAYFERQLDLLESRAHQRLNSQVRNVWEAKNDWNFSPDSSQ
                     AEVERFLDHLLELGDGESAHLISAKNEWIRFRKDSETPFPSLVLKRLADTQRILALHQ
                     LLRNHDICLRHGLPSIAPSADFWGKDL"
BASE COUNT         1113 a         1369 c         1604 g         1078 t
ORIGIN      
        1 tccgaagtgt tggcgttgat cgagtctctg cgattgccag cctcacgtcc ggcgggcccg
       61 taagctcgag taccggttca tgaatgctcg caaaggctgc cagagcaata atcagagttt
      121 gaagatcacc cgtgttgacc cgtgacggtc gtgtttgtcg tgccggtgtt ttcgtttgtg
      181 atccacgacg acgaccgggg catgaatcgg ccggcgtttg ggagagcggg tgaagcagaa
      241 aggggccgga ccgggaacca ctatctgaaa ctgtggtctc caggtggtat gacgttcgag
      301 atcgccgatg gtgacccagc aggagatggg ccggcagggg ttcgagttgc tgacgaacat
      361 cacccgggcc tgactgcctc gaacctcgag cacgcgaatc ggatggtgtc gatcgaggcg
      421 ctgactgttc agatacagat aaggcgaggt gaccagatac gccggcgcga cgtcggttgt
      481 catgattggt gtgaccacct gcaccggctg aaccatcgga ggcgctgagg ggctgacagt
      541 cggaaccgga gacgtatcga tcgaggatga gccgtcgcgg caggctttac cgcaggatga
      601 gcagaagttg gcatcgtctg ccaggttgtg accgcattgc gaacagaaga gagcgaaggc
      661 agggcccgtg gtcagcagaa gaaccatcag ggtcagaatc agacgtcgtt tcatggcaac
      721 ctcccagagt cgattggtcc catcattgac gccgtatcgt cctcgcggat tgttcgaaag
      781 cgttaagaaa tgtaaacccg tcctgtcgca gggcagatct ctggactcgg ttgaggactc
      841 ttgctccggc gtgggttcct cgttatcatg gcgcggactc ggcagaatct ttcccggttg
      901 aggagacgct atggcggaac aatatattta cacgatgatg gggttgagca agttctatgg
      961 gcagaaacag gttttgaaag agatcaacct ctgtttttac ccgggtgcca aaatcggcat
     1021 cgtcggtgaa aacgggtcgg gtaaatcgac gctgttgcgc atcatggccg gtatcgacaa
     1081 ggatttcgtc gggcactcgg agccggcaaa aggcgtgcgg gtcgggttcg tgccgcaaga
     1141 gccgcaactg gcactgggca agacggtgcg cgaaaacctg gaaatggcgg tcgccgacca
     1201 catgaagctg ttgaaggaat acgaagacat ctcgaacagc atgggcgaga tggatggcga
     1261 tgcgatggat aaagccatgg aacgcatggg cgttctacag gacaagatcg aagccgcggg
     1321 cggctgggag atcgataccc gcctcaacgt cgcggccaac gccctcgtgc tgccccctga
     1381 cgacatgatc gtcgacaacc tctcgggcgg ggaaaagcga cgcgttgcac tctgccgggc
     1441 gttgctcgaa cagcccgatc tgcttctgct cgacgaacct accaaccacc tcgatgccga
     1501 gaccatcgac tggctggaaa actatctcaa ggactatccc ggcaccgtca tcatctcgac
     1561 gcacgaccgc tacttcctcg acaacatcac caagtggatt ctcgaactcg aagagtgccg
     1621 cggtattccc ttcgaaggca attacacttc ctggctggcc cagaagctcg agattctggc
     1681 gcagggcgaa aagaaggaat cttcccgccg ccgctcgatc gatcgcgagt taaagtggat
     1741 caagatgaac acggccgatc ggcacgaact caaccgcaac cgcatcgctc agtacgagca
     1801 gttggtcgcg cgagagaagg cggagaacaa ggttgacacg accgtcattc agatcgctcc
     1861 gggcgaagag ctcggaaacc agattgtcga ggcgcgaaac gtgttcaagg gctacgggaa
     1921 gaatgaactg atcaaggatc tgcagttcaa cctgccgcga ggcgccgtcg tcggcattat
     1981 cggcccgaac ggcaccggca agaccacgct gttcaagatg atcgccgggg ttgaaaaacc
     2041 cgacaaggga gaagtcatcg tgggtccgtc ggtgcgcctg gcctgggtcg aacagtttcg
     2101 agacagcctc gaaaacggca aatcggtcta cgacgtcatc agcgaaggca aggccgagat
     2161 tcccttcggc aaagtatcga ttccggcccg cgcttactgc ggtcggttcg ggttcaaggg
     2221 cagtgatcag cagaaactgg tcgagaatct ctcgggcggt gagcgcaacc gggttcacct
     2281 ggccaaggta ctgaaatcag gcggaaacct ggttctgctc gatgaaccga ccaacgatct
     2341 cgacgtgggt acgctgcgta tgctcgaaga cgcggtgacc gatttctcgg gttgtatcat
     2401 ggtcatcagc cacgatcgct tcttcctgaa ccgcatctgc acgcatctgc tggtattcga
     2461 aggcgactcg aaggtcacgt ggttcgaagg caactacgag gaatacgaaa cgaaacgtcg
     2521 caaggaactc ggcgcggccg cggtcgaaaa ccggcgggcc cgctatcgca agttcgccat
     2581 caactgaccc ggcgaagatt cccgggaaac aacaccggcc ggctcttctc gcgaagaacc
     2641 ggccggtgtg tctgtgcggg aaatcactgg gtgtgctggt tcggggtcag gcgggcaatc
     2701 tgagtcaggg cgcgctcggc tccttcgcga atctccggat cggggtcgac gccggccagg
     2761 cgttcgagaa cgggctgagc gctgggatca cgcaacaggc caagcccttt gaccgccaga
     2821 cttcggacgg ccgggcgcgg atcggtcgcc agaaactgca ggggttcggc gaaacgacgg
     2881 tctgcggact tcggcaggta ggatcgcacc gcggtttcga gggcaagcgt ctgcacgcga
     2941 ggttgatagt cagaaagggc cttgagcagt gcttcgccga cccagggagc ggtgccggtg
     3001 tgattcaggt tttctacagc cagttgtcgc agactggtga ccgggtcaga gagagccttc
     3061 agatgaaaat caagaaagtg aggtgcctgc cgggtgcgta agccttcaat agcggcgcgg
     3121 cgaacggcct gactggggtc attgagaaga gaacggtgga gagttgtgag ggaagagtca
     3181 tcgatggtcg tgagcagctt ggcgccctgg agtcgaatgg cgggatcatc gctctgcatt
     3241 tttccgcgag cgacctcgac ccaatcgagg gatttcaggc ccagcgcttc gagcatggct
     3301 ccaccggcga agagaaacag ggtgaaggtc agcgtcagca gcagcgaaaa tgcgatggtt
     3361 tctgggttcc agcgggacat gcgggcctcc gggaaatcgg actgtctcgg cacttcaacc
     3421 tctggacatg ggtaatatag cctgtttttt cccgacgtca agggtgtttc gaatctgtta
     3481 aaattctgaa tacttctctt ctcacagaga ggcaaatcgt tcccgggatt ccacgatcgt
     3541 atgggcacat ctgtcgcagc tgccgaacaa aacgtcattg cggcggtcgg atattcccga
     3601 ttccagaccc tactctcctg gtgtctgatc tcagtagtcc cgatactatt cctgactctg
     3661 gccatatccc ggatcaccca tgaagaatca tggagtctgc gtctggccgc agaacgtcgg
     3721 ttgatgagtg agatggccgg atatctccgc gacattgagc cggatcaggt gctggcgcgg
     3781 gttctcagaa aggctgatga agcatgtgat ccacgatgga catcagatca ccacttcact
     3841 gcgcagggca acgaaggctg gagcgatcct ggccgggtcg tttcagtggt gaaagaagcg
     3901 attcgacgcg atcttggagt cgagcctctg ctcatggtgg ctgtgggaac gggctacgat
     3961 acaatcgtga ttcacaccga cccgcgtgcg ttcggtgatc gggtggctcc atcccagact
     4021 gcctggagac aatggttctt tcacgtcagt gaaggaagaa aacgccaggg ttcaccggta
     4081 tcgctttctg attcccgact tcgggcaaag tatgcgaccc gggaacaatg gcagcagctc
     4141 ggcgaattct gtcgcaagtt tctgcagagt cagttcggtg actatattcc agaagtgttg
     4201 atcaacggac ggccaacatc ctttttcagt cccaagttcg gaggtgggag gctggtgatc
     4261 gcctatcgcc tgtttccggg agccccggat ccgagtgacc gttttccggg aggatatgtc
     4321 ctgatcatgc gagatgccga aatcgcccgc gaagcattgc tgcgtgggtc agcggcatct
     4381 gggcgcgcct ggcgtcgcga agcagtggtg agtgagccgg catttcgcga gactccggag
     4441 tctctggatt atgttcatcc tgcgccaccc gccttgtttc cggtgttcga gcacggaaac
     4501 gcttcaccga cgttcctgca cctgatcgtg tctgttccgt acaatcaatt gatcccgtcg
     4561 ctgcttgcct ggaagattcc gggaatcgtt ctggcagttc tttggttgca tttcggatgg
     4621 atatttctac ggcgtctgca tgatgggacg ttgcgtctca atgtgcgtat tgccgggcag
     4681 ttgcgtttcg gacttctgtt gatggttgga ttgtcgctcg ttgccctgtt gctgtttgcg
     4741 cgttcctacg gttcgctggt cgagcaaagt gaacagtggc agcgaaaagc ctatttcgaa
     4801 cggcaactcg acctgttgga gagccgcgct catcaacgct tgaacagtca ggttcgcaat
     4861 gtctgggagg cgaagaatga ctggaacttc tcgcccgact cctctcaggc tgaggtggaa
     4921 cggtttttgg atcatcttct cgagctgggg gacggcgaat cggcacacct gatctcggcg
     4981 aagaatgaat ggatcagatt ccgcaaggat tcggagactc cgtttccctc tctggtcttg
     5041 aagcgtctgg cggataccca gaggatcctg gcattgcatc agctgttgcg aaatcacgac
     5101 atttgcctcc gacatgggct tccctcgatt gctccttcag cggacttctg gggaaaagac
     5161 ctgc
//
LOCUS       DAORHD010000154         5162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16769_length_5162_cov_3.541218, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000154 DAORHD010000000
VERSION     DAORHD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16769_length_5162_cov_3.541218"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..801)
                     /locus_tag="PKO06_04640"
     CDS             complement(145..801)
                     /locus_tag="PKO06_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV68964.1"
                     /translation="MKLGIFSDVHANLQALQACLLEMARLGCHRLICLGDVATLGREP
                     IATLRLLMNLECLFIRGNHDRYLLDPASIDQYDTVELVRRDVRNVASLLHDEDRVFLR
                     SFVPHATLELAGTRLCFFHGSPISDEIMLLPHLPKEKLFESTSSVDADWYIGGHIHST
                     YTLDFKGKRLINAGSLGAPFVGWNPAKNEPLFGSDGSFLTIEAMGNNLSVTFHTVPLG
                     "
     gene            complement(833..2686)
                     /locus_tag="PKO06_04645"
     CDS             complement(833..2686)
                     /locus_tag="PKO06_04645"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68965.1"
                     /translation="MKEYTLPLILLFIVVICCGGFYQFYVKPTTTVKPMSVKLSIDEI
                     KTLDEAEQLLEQGKAGEAWHRLEPMRSEPLRTRLLKTEAQLKTGKSPATEIGDLEREV
                     AKDRSLELVYVLARIHEASRDQRKARGLYEELLKASLRQELRLRVQQRYVSLCLSQKR
                     LADAVQTLRQSAGKLLVSEDDATSMLQVIVETKSDAILEELPQLVATSTRRAVRFQFA
                     VANAWNALKQRDKAKHCLLEALKLQPDHLPSLYVLFEFSRQGSSFDDAREYLQRMLLS
                     GQTLPERKDWDKLLLTFAGKAHEARNLPQSFLFLKHAVFLNRSSLGADIQSILHDCES
                     YYRENGTDNEQAFFPVFLSFANGEYALALSGAERLLKSVTDAPMKTQIEIMVRESKQA
                     MEREQAALAKPATTSKPLPPVGVPASAPVRLASSSVRVASAPVDPTVPPTDPEEQEWM
                     TAMKAKFKEKAEDPVALLADAKELAAARAWNGAAFYLEAILKADPHHEETLKLMARVQ
                     GKRGKHEEAVVLLEGLLQSNPQDALLRASLARNLIKTKDASRGLREARLALELDPHQL
                     EGYVALGEYFEMRQRPTEALENVNNGLLKVTDSNAEAAQALYELKARLEKK"
     gene            2930..3334
                     /locus_tag="PKO06_04650"
     CDS             2930..3334
                     /locus_tag="PKO06_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68966.1"
                     /translation="MQVSAGSLTVGTKVIYPGGPDQPPKEKDTGVSLKAEVVGISDES
                     DVADPTSASATEDLALLAESGRYEDLLEQVEEPKTTEQIAWKALALWETGSEHEARVL
                     ATQALKEKNLPRHLREKLETTFEVQNESEPAE"
     gene            complement(3403..4416)
                     /locus_tag="PKO06_04655"
     CDS             complement(3403..4416)
                     /locus_tag="PKO06_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNV68967.1"
                     /translation="MKTGFSKVFGCTLLGLSLSFSGTASADTPATNTVLTSRAAVTQP
                     RTADPTRRVETPDFVAILQTLEQLESSLPTKSDLITRLRGQLYRYERQSQREKFDLVG
                     ELRKARQTTTSRLPGSSTRKPGDWQREPEPGNNLYTPCVGLTNEALKEKLQSITEYQV
                     GLDYREARRQMFTRLDNVGGSVECVYTGRKGRYSALPNDRDMNCEHTWPQSHGAVGIA
                     KSDLHHLFPTDSEANSKRGSLPFGPVNSAEWAEGGSKCDGDVFEVRPEQRGNTARAKF
                     YFAVRYGKRIESEEEATLREWHKQDPVDDFERRRNDLIENLQHNRNPFVDHPEFVDQI
                     SDF"
     gene            complement(4567..>5162)
                     /locus_tag="PKO06_04660"
     CDS             complement(4567..>5162)
                     /locus_tag="PKO06_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNV68968.1"
                     /translation="QSLSLAFGPQNFLVTYHAATRGDEVPAHAFAEILEALEAFPQSH
                     VVFTGSNADTNGRIIGSLIEDWVRRFPSRAHFFHNLGYRRYVSLMRQVDAVIGNSSSG
                     LIEAPAVGVPTVNVGDRQKGRLRAPSVIDCIPRAENVKDAIRRALIPEFRDAARTQPH
                     PFGNGTASARILQTLKQQSLEVGAPKSFFAIPLTKDL"
BASE COUNT         1130 a         1405 c         1452 g         1175 t
ORIGIN      
        1 acagagccac gcgaaaccaa gtcctcatac gcagcctcca cgagataaaa tgacgatctc
       61 ttctttcata ttactgcaga acatgcagaa acgtttacag atatttttac tcacgtgccg
      121 ggttattctc cggcggaact cgttctaccc cagcggaacc gtatggaaag ttaccgagag
      181 gttgtttccc atggcttcaa tggtgagaaa acttccgtct gagccaaaca gcggctcatt
      241 ctttgccgga ttccaaccaa cgaatggcgc accgaggctc ccggcattga tcaggcgctt
      301 tcccttgaaa tcgagggtgt aggtcgaatg aatgtggcct ccgatgtacc agtcggcatc
      361 aacggagctc gtcgactcaa aaagtttttc cttgggaaga tgtggcagaa gcatgatttc
      421 atcggaaatg ggtgaaccat gaaagaagca taaccttgtt ccggcaagtt ccagcgtggc
      481 atgaggaacg aacgagcgaa gaaagacgcg atcttcgtca tgcagcagag aagctacgtt
      541 gcgaacgtcg cgacgtacga gttcaaccgt gtcatactga tcaatcgaag cgggatcgag
      601 gagataccga tcgtgattcc cgcgaatgaa cagacactcg agattcatca gcagtcgcag
      661 cgttgctatc ggttcgcgac caagggtggc aacatcgccc agacagatga gtcgatgaca
      721 tcccagtctt gccatctcaa gcagacatgc ctgcagtgcc tgaagattcg catgaacgtc
      781 cgagaagatc cccagcttca tggtttatga tgagacctgt gtctggcagg cgtcatttct
      841 tctccagacg tgctttcaat tcgtacaacg cctgcgccgc ttcagcattc gaatcggtga
      901 ccttcaacag gccgttattg acgttttcga gcgcttcggt ggggcgctgc cgcatttcga
      961 aatactctcc gagagccaca tatccttcga gctgatgcgg atcaagttcg agggccagtc
     1021 tggcttctcg cagtccgcgc gaagcatctt tcgtcttgat cagattacga gccagactcg
     1081 cacgcagcag ggcatcctgc gggttactct gcagtaatcc ttcaagcaga acaaccgctt
     1141 cctcgtgctt tcctcgtttg ccctgcacgc gtgccatcag cttgagggtt tcttcgtgat
     1201 gcgggtcggc tttgaggatg gcttcgagat aaaaggccgc tccattccag gctcgagccg
     1261 cggcgagttc tttggcatcg gcgagtaacg cgaccgggtc ctcggccttt tccttgaact
     1321 tggctttcat cgccgtcatc cactcctgtt cttccggatc ggtgggtggt actgtcggat
     1381 caaccggcgc cgaggcgacc cgaacggatg acgaagcaag gcgaaccggt gccgaggcgg
     1441 gaacgccgac ggggggaagc ggcttcgatg tcgtggccgg cttcgccaac gctgcctgtt
     1501 ctcgttccat cgcttgcttg ctctcacgca ccatgatctc gatctgggtc ttcatggggg
     1561 cgtccgttac ggatttcaac aggcgctccg ctcccgagag agccagcgcg tattcgccgt
     1621 tggcaaatga aagaaacacg ggaaagaagg cctgttcgtt gtcggttcca ttctctcgat
     1681 agtacgattc gcaatcgtgc aatatgctct gaatatctgc accgagactc gaacgattca
     1741 gaaagacggc atgcttcaga aagagaaacg attgcggcag attccttgcc tcgtgcgctt
     1801 tgccggcaaa ggtcaacagc aacttgtccc agtctttgcg ctccggcaga gtttgtcccg
     1861 aaagtaacat gcgttgcaga tactcgcggg catcatcgaa tgaacttccc tgacgcgaaa
     1921 attcgaaaag cacatagaga ctcggcaggt gatccggctg aagtttcagt gcctcgagaa
     1981 gacagtgttt cgctttgtcc cgctgtttca gggcattcca ggcgttggcg accgcaaact
     2041 ggaatcgcac cgcacgtctg gttgacgtgg caaccaactg tgggagttcc tcgagaatgg
     2101 catcagactt ggtttcaacg ataacctgca gcatactggt ggcatcgtct tcagagacga
     2161 gcagcttccc ggcgctctgg cgaagcgttt gaacggcgtc ggccagccgc ttctgcgaga
     2221 ggcatagtga aacataccgc tgttggaccc gtaagcggag ttcctgacgc aacgaggcct
     2281 tgagcagttc ttcatagaga ccgcgcgctt tccgttgatc acgactggct tcgtgaatgc
     2341 gcgccagaac atagacgagt tcgagactgc ggtccttggc gacttcgcgt tccagatcgc
     2401 cgatctcggt ggcaggagat tttcccgttt tcagctgggc ctcggtcttg agcagtcgtg
     2461 ttcgcagcgg ttccgaacgc atgggttcga gtcgatgcca ggcctcgcct gcctttccct
     2521 gctccagtag ttgttcagct tcatcaagtg ttttgatctc atcgatactc aacttgacgg
     2581 acatcggctt cacggtggtc gtcggcttca catagaactg atagaagcca ccgcagcaga
     2641 tcacgacgat gaacagcaaa atcagaggaa gggtatattc cttcattggt ctagagtatc
     2701 aaattcccac gtcgcttgtc ctgatttctc cggtcaaaac aagtgacacg cgtgacagtc
     2761 gtgtgatcta tcgagggctt tctggtattg cccgagccgc cttcattctg gtaggcttcc
     2821 cgtgatgcca ctcagaattc gatggtctga tacgtgtgaa gtttcgatac ggaatgtgtc
     2881 gaaaacggcg gcggcgtgcc tgctttcggc gtgtcttctg ataacaccaa tgcaggtgtc
     2941 agccggaagt ctgaccgtcg ggacaaaagt catctacccg ggcgggcccg atcaaccacc
     3001 gaaggaaaag gataccggcg tctctctgaa agccgaggtt gtcgggatca gcgacgagag
     3061 cgatgttgcc gacccaacgt cggcatccgc gacggaagat ctggcgctgc tggcggaaag
     3121 cgggcggtat gaggaccttc tggaacaggt cgaagaaccg aaaaccacgg aacaaatagc
     3181 ctggaaggct ctggctctct gggaaaccgg cagtgagcac gaggctcgcg tcctggcaac
     3241 tcaagccctg aaagaaaaaa atctgccgcg gcatctgcga gaaaaactcg aaaccacctt
     3301 cgaggttcaa aacgaatcag agccggcaga gtaaccggcg cacccaaaac agaagccccc
     3361 ttcgtggaaa acgaaggggg ctttctgcgg tcttgaaaac gatcagaagt cgctgatctg
     3421 atcgacgaac tccggatggt cgacaaacgg attgcggttg tgctgcaggt tttcgatcaa
     3481 atcattgcgc cgacgctcaa aatcatcgac cgggtcttgc ttatgccatt cgcgcaacgt
     3541 ggcctcttct tcagattcga tgcgcttgcc gtagcgaacc gcgaaataga acttggcgcg
     3601 agctgtgttt ccgcgctgtt ctgggcggac ttcaaacacg tcgccatcgc actttgagcc
     3661 gccttcggcc cactcggcgc tgtttaccgg accgaacggc agtgagccgc gcttgctgtt
     3721 cgcttctgaa tcggtcggaa acagatgatg cagatcgctc ttggcgatac caaccgcccc
     3781 gtggctctgc ggccaggtgt gttcgcagtt catgtcacga tcattcggaa gcgcgctgta
     3841 tcggcctttt cgacccgtgt atacgcactc gacagaaccg ccgacattgt cgagacgggt
     3901 gaacatctgt ctgcgggctt cgcggtagtc gagaccgacc tgatactcgg tgatcgactg
     3961 cagtttttct ttcagtgctt cattcgtcag tccgacgcac ggggtataca gattgttgcc
     4021 cggttccggc tcgcgctgcc aatcgcccgg tttacgggtc gaactgcccg ggagtcgact
     4081 ggtggtggtt tgacgggctt tgcgaagttc tccaaccaga tcgaacttct cacgctgaga
     4141 ctgtcgctcg tatcgataca actgaccgcg caatcgcgtg atcagatcag acttcgtggg
     4201 cagactggat tccagctgtt cgagagtctg cagaatggca acgaaatcgg gagtttcgac
     4261 gcgccgggtc gggtctgcag ttcggggttg cgtcaccgcg gctcgagacg tcagaacggt
     4321 gttcgtcgct ggagtgtcgg ctgatgcggt tccggagaac gacagagaaa gaccgagcaa
     4381 ggtacaacca aacactttgc tgaagccggt tttcatgcag gtttcctttt actcatcgtg
     4441 agaagtgtct gaatgaattc tcattatacc accgcactac cggggtctgg ggaagtttgc
     4501 cgcaactttc gggcgataag tccgttgttg tgtctgccct cgcccgaaca actggtctct
     4561 caaccttcac aggtccttgg tcagtggaat ggcgaagaaa ctcttcggcg cgccgacctc
     4621 aagagattgc tgcttcagtg tttgcagtat ccgtgcactc gcggttccat ttccgaacgg
     4681 atgaggttgc gttctggctg cgtcccgaaa ctcgggtatg agcgctcgtc tgatggcatc
     4741 cttcacgttt tcggcacgtg gaatgcagtc aatcactgac ggggcgcgta accgcccttt
     4801 ctggcggtct ccgacattca ccgtcggcac tccaacggcg ggagcttcga tcagaccgct
     4861 ggacgagttg ccaatgaccg cgtcgacctg acgcatcagg ctgacgtacc gccggtagcc
     4921 cagattgtga aaaaagtggg cacgtgacgg aaaacgtcga acccagtctt cgatcaggga
     4981 accgatgatt cggccgttgg tatcggcatt cgatccggtg aaaacgacgt gagactgcgg
     5041 aaatgcttca agcgcttcaa gaatctcggc aaaagcgtga gccggaactt catcgccgcg
     5101 tgttgcggca tggtaggtca cgagaaaatt ctgcgggccg aacgcgagtg aaagactctg
     5161 tt
//
LOCUS       DAORHD010000155         5129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16926_length_5129_cov_4.694718, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000155 DAORHD010000000
VERSION     DAORHD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16926_length_5129_cov_4.694718"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1236
                     /locus_tag="PKO06_04665"
     CDS             154..1236
                     /locus_tag="PKO06_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNV68969.1"
                     /translation="MADKILMKGNEAIGEAAVLAGCRHYFGYPITPQSELPAYLSKRL
                     PEVEGCFLQSESEVAAINMVFGAASAGARVMTSSSSPGVSLMQEGISYIAAAELPCVL
                     VNVVRGGPGLGNIAPAQSDYFQSTRGGGHGDYRTIVLAPNSVQEMTNLTVKAFEAADK
                     FKNPVLLMVDGVLGQMMEPVQLPEPVNYDPAAKKWALTGKGARKERNLCTTIWLDVEL
                     LEEHNWHLYQKYQYITDEFTTGSWKEYGCELHQTEDAEIVLVGYGIISRILKTVVEKC
                     RKRGIKAGLVRPITLWPFPTPSIQKLVPSAKHFLCVEMSTGQMVEDVKLAVEGKKPVY
                     FYGRTGGSLPSDFEILNEIDKIVRPD"
     gene            1250..2005
                     /locus_tag="PKO06_04670"
     CDS             1250..2005
                     /locus_tag="PKO06_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV68970.1"
                     /translation="MQKIFERPKALSKNPFHYCPGCDHGVIHRMVAESLDELEVTNKS
                     IGVAPVGCSVFAYDYFDIDFNSAPHGRAPAVATGIKRVHPDSIVFSYQGDGDCAAIGT
                     AEIVHAANRGENITVVFVNNAIYGMTGGQMAPTTLVGMKTATSPYGRDAKTMGHPIKM
                     SEMLSTLTAPAFVARVSIHNVANLMKAKKAIKRAFEVQKEKKGFSFVEVLAICPTNWG
                     KPPVEAHKWLEENMVPVFPLGVFKDQTGGPAVL"
     gene            2030..2581
                     /locus_tag="PKO06_04675"
     CDS             2030..2581
                     /locus_tag="PKO06_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV68971.1"
                     /translation="MYPNSEEIIMAGFGGQGILFLGKVLAEAGMRSGRHVSWIPSYGP
                     EMRGGTANCTVVIAKEPIGSPMVNEPDCVLALNKPSIEKFAKTIRQGGVLVYNSSMSD
                     QKPEKDGVKCIPVPASKIAEELGSGRVTNLVMVGAFAKYSQMVKFDDLVKWLPQIIPA
                     KKKELAEINLNALKKGYEYVSSH"
     gene            2800..4134
                     /locus_tag="PKO06_04680"
     CDS             2800..4134
                     /locus_tag="PKO06_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV68972.1"
                     /translation="MGHVTSKSYLSLQKRLDMHGQGVPASETLFRILEHLFTEQEASF
                     VSQLPLNFFTLDQAVRITGKTEAEAAAILDGLADKAILVDVESAQTKAYIVSPPMAGF
                     FEFSLMRTDGKFDRQLLSHLFYQYINQEDEFMFQAIGMDSHIIRTFVHEDMIEKDGLT
                     MVLDYEKASHVIESASCITLGTCYCRHKMEHVGKACEFPQDVCMTFNTLATSLSKHGV
                     AREIAKGQALDILHRCMSLGLVQLGDNVQEGVNFMCHCCGCCCEALTNYERLRFAPKF
                     ASNFTATIREPSCTGCGICERKCPVKAVTIATHHGRRVAEINREICIGCGVCKRFCPR
                     QSIELERLKALRFVPKDSFERHLVFNIERGCLENLILDNLEFWGHQQVRALLKAILTS
                     APVKKALLNHRIRSRFINLIIHHTGKYDLYDRLFNDGRKVDYSHPELRQHPS"
     gene            4224..5060
                     /locus_tag="PKO06_04685"
     CDS             4224..5060
                     /locus_tag="PKO06_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNV68973.1"
                     /translation="MEYVSNKETLVGWRRTWHEIIFEADTPAGKAFDVTLITLILSSV
                     LLVMVDSVAHIHQQWGEALRWFEWGFTLLFSLEYLMRLNCVTRPLAYATSFYGVIDLL
                     AILPSYLGLVVAGSQYLMAVRVLRVLRVFRIFKLTQYLAEANLLLRALRASLPKIVVF
                     TLTVSTLVIVIGSVMYVIEGPENGFTSIPIGIYWAIVTLTTVGFGDVTPKSGLGQMFA
                     CIVMILGYGIIAVPTGIVTAELTREARESFKKVSTQACMSCSLDGHDADAAYCKACGA
                     KL"
BASE COUNT         1107 a         1297 c         1592 g         1133 t
ORIGIN      
        1 atgaaggttc tgggcgtggc ggagcatctg aacaagaagg gttttcaccc ggcgacgatg
       61 ctgaacgagg cgaagtgtac gagttgcggg atctgcggtc gaagttgccc ggatgtcgcg
      121 atcgaagtga acaagccgta aggaggccga tcgatggcag acaagattct gatgaagggg
      181 aacgaagcga tcggcgaggc cgcggtcttg gcgggttgcc gacattattt cgggtatccg
      241 atcacgccgc agagcgaact gccggcgtat ctgtcgaagc gcctgcccga ggtcgagggc
      301 tgctttctgc agtctgagag cgaagtggcg gcgatcaata tggtgttcgg cgcggcgagc
      361 gcgggggctc gggtgatgac gagttcgagc agcccgggcg tgagcctgat gcaggaaggc
      421 atctcgtata tcgcggcggc ggagctgccg tgcgtgctgg tgaatgtggt gcgtggcggc
      481 cccggtctgg gcaacatcgc tccggcgcag tcagactact ttcagtcgac gcgcggcggt
      541 ggccatggcg attaccgcac gatcgtgctg gcgccgaact cggtgcagga gatgacgaac
      601 ctgacggtca aggcttttga agcggctgac aagttcaaga acccggtact gttgatggtc
      661 gacggggttc tgggccagat gatggagccg gtgcagctgc ccgagccggt gaactatgac
      721 ccggcggcga agaagtgggc tttgacgggc aagggtgctc gcaaggagcg gaacctgtgc
      781 acgacgatct ggctggatgt cgaactgctg gaagaacata actggcacct gtatcaaaag
      841 taccagtata ttaccgatga gttcacgacc ggaagctgga aagagtatgg ttgtgagttg
      901 catcagacgg aagacgcgga gatcgtgctg gtcgggtatg gcatcatctc acgcattctg
      961 aagaccgtgg tcgagaagtg ccgcaaacgc ggtatcaagg ctggtctggt gcgcccgatc
     1021 acgctgtggc cgttccccac gccgtcgatt cagaagctgg tgccgagcgc gaagcacttt
     1081 ctgtgcgtcg agatgtcgac gggccagatg gtcgaagacg tgaagctggc ggtcgaagga
     1141 aagaagccgg tgtatttcta cggtcgcacg ggcggctctc tgccttccga cttcgagatc
     1201 ctgaacgaga tcgacaaaat cgttcggccg gactaaggag accatgacga tgcagaaaat
     1261 cttcgaacga ccgaaagcat tgagcaagaa tccgttccat tattgtccgg ggtgcgatca
     1321 cggcgtgatc caccggatgg tagcggaatc cctggacgaa ctggaagtga cgaacaagtc
     1381 gatcggcgta gccccggtgg gttgctcggt gttcgcctat gactacttcg atatcgattt
     1441 caactcagcg ccgcatggcc gcgctccggc ggtggcgacg ggaatcaagc gggttcaccc
     1501 ggactcgatc gtgttctcgt atcagggcga cggcgactgc gcggcaatcg gcacggccga
     1561 gatcgtgcat gcggcgaacc gcggcgagaa catcacggtg gtgttcgtga acaacgcgat
     1621 ctacggcatg acgggtgggc agatggcgcc gacgacgttg gtcggcatga agacggcgac
     1681 ctctccgtat ggtcgtgacg cgaagacgat gggacatccg atcaagatgt ccgagatgct
     1741 gtcgacgctg acggctccgg cgttcgtggc tcgcgtgtcg attcacaacg tggcgaacct
     1801 gatgaaggcg aagaaggcga tcaagcgggc tttcgaggtt cagaaagaga agaagggctt
     1861 ttcattcgtg gaagtgctgg cgatctgccc gacgaactgg ggcaagccgc cggtcgaagc
     1921 gcacaaatgg ctcgaagaga acatggtgcc ggtgtttccg ctgggggtct tcaaggacca
     1981 gaccggcgga cccgcggtgc tgtgagcctg gaatagggag gaaaagtaca tgtatccgaa
     2041 ttctgaagag atcatcatgg ccgggtttgg cgggcaggga attctgttcc tgggcaaggt
     2101 gcttgccgag gcgggcatgc gatcaggcag acatgtttcc tggattccca gctacggccc
     2161 ggaaatgcgt ggtggcacgg cgaattgtac ggtggtcatc gccaaagagc cgatcggctc
     2221 cccgatggtg aacgaacctg attgcgtgct ggctctgaac aaaccgtcga tcgagaagtt
     2281 cgccaagacg attcgtcagg gcggagtcct ggtgtataat tcgtcaatgt ctgatcaaaa
     2341 gccggaaaag gacggggtga aatgcatccc ggttccggcc agcaagattg ccgaagaact
     2401 gggcagcggt cgggtgacca acctggtcat ggtcggcgct tttgccaaat acagtcagat
     2461 ggtcaagttt gatgatctgg tgaaatggct gccgcagatt attccggcga aaaagaagga
     2521 actggcggag atcaacctga atgccctgaa gaagggttac gagtatgtaa gcagccactg
     2581 aacgatcgag cgttaccatc aggccgggtt cctctgggag cccggtcttg cttttttctg
     2641 aatcgcggag tgccggactc atcttgcggc aagccactct caacgactga ccagtcaggc
     2701 ggaaacagac aagcgctctc ggcttcggaa aaacttgacg aatgagcaaa ctttgtctta
     2761 tttttcgaag acattcttga gaacgctgtg gggatcaaca tggggcacgt gacctcgaaa
     2821 tcctacctgt cgctgcagaa acgcctcgac atgcacggtc agggagttcc tgcttcagag
     2881 acgttgtttc gcattctgga gcatttgttc acggaacagg aggcatcttt tgtttctcaa
     2941 ctgcccctga actttttcac tctcgatcag gcggtgcgca ttaccggaaa gactgaagcg
     3001 gaagccgcgg caattcttga tgggcttgcc gacaaggcga ttctggtcga cgtcgaatcc
     3061 gctcaaacca aggcttacat cgtgtcaccc ccgatggctg gtttcttcga attcagcctg
     3121 atgcgtacag atggaaagtt tgatcgacag cttttgtcac atcttttcta ccagtacatc
     3181 aaccaggaag acgagttcat gttccaggcg attggcatgg acagtcacat cattcgcacg
     3241 tttgtccatg aagacatgat cgagaaagat ggcctgacga tggttcttga ctacgagaaa
     3301 gccagtcatg tcattgagag tgccagctgc attaccctcg gcacatgtta ctgtcgtcac
     3361 aaaatggagc atgttggcaa ggcatgtgag tttccccagg acgtctgcat gacgttcaac
     3421 actctggcga cgagtttgtc gaagcatggg gtggcgcgag agatcgcgaa ggggcaggcc
     3481 ctggacattc tgcatcgatg catgtcactt ggattggtgc aacttggcga caatgtccag
     3541 gaaggcgtca atttcatgtg tcattgttgc ggttgctgct gtgaagcgtt gacgaactac
     3601 gaacgcctga ggtttgcccc caagttcgcc agcaacttta ccgcgaccat tcgtgaaccg
     3661 agttgtaccg gttgtggcat ctgcgagcga aagtgtccgg tgaaggcggt aaccatcgcc
     3721 acgcatcatg gacgacgcgt cgcggagatc aatcgtgaaa tctgcattgg ctgcggtgtc
     3781 tgcaagagat tctgccctcg tcagagcatc gaactagagc gactgaaggc gctgcgcttc
     3841 gtgccgaaag actcgtttga acggcatctg gtcttcaaca tcgagcgcgg ttgcctcgaa
     3901 aatctgattc tggataacct cgagttctgg ggccaccagc aggtgcgcgc tctgctcaag
     3961 gctattctta cgagtgctcc tgtaaagaag gctctgctca accatcgcat tcgctcgcga
     4021 ttcatcaatc tgatcattca ccacacagga aagtacgact tgtacgaccg ccttttcaac
     4081 gatggtcgca aagttgatta ttcgcatccg gagctgcgcc agcatccttc ctgacgagag
     4141 agccggaagc gacggaatct caccttgacc cggagacggg gttgcgacca tacttggtca
     4201 ttctgtacag ggggctgggg ttcatggaat acgtgagtaa caaggaaaca ctggtcgggt
     4261 ggcgcagaac ttggcacgag atcatcttcg aagccgacac ccccgcagga aaagcgtttg
     4321 acgtcactct gatcacgctt atcctgagca gtgtgctgct ggtcatggtg gatagtgtcg
     4381 cgcacattca ccagcaatgg ggagaagccc tgcgctggtt cgagtggggc ttcacgctgc
     4441 tcttttccct ggaatacctc atgcgtttga actgcgtcac gcgccccctt gcgtatgcta
     4501 ccagcttcta cggggtgatc gatctgctgg cgattcttcc ctcgtatctc gggctggtcg
     4561 ttgccggctc acagtacttg atggccgtgc gcgtcctgcg ggtgttgcga gtgtttcgta
     4621 tcttcaaact gacgcagtat ctggccgagg ccaacctttt gttacgtgct ttacgtgcca
     4681 gcctgccgaa gatcgtcgtg tttacgctga ccgtctcgac tctggtgatt gtcatcggtt
     4741 ctgtgatgta tgtcatcgag gggccggaaa atggctttac cagcattccg atcggtatct
     4801 actgggctat tgtcacgctg acaaccgtcg ggttcggaga tgtgacgccg aagtccgggc
     4861 ttggacagat gttcgcgtgc atcgtcatga ttctggggta tggcatcatt gcggtaccaa
     4921 ccggtattgt cacggcggaa ctgacccgtg aggcacgtga atcgttcaag aaagtcagta
     4981 cccaggcctg catgagttgc tcgctcgatg ggcatgacgc agatgccgct tactgtaagg
     5041 cttgtggtgc caagttgtaa tcctgtcggc gatgccccag ggtcatttaa ttctcctcct
     5101 tacgtgtttt ccgttcacgt cgctcaacg
//
LOCUS       DAORHD010000156         5124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16952_length_5124_cov_4.197869, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000156 DAORHD010000000
VERSION     DAORHD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16952_length_5124_cov_4.197869"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2585
                     /locus_tag="PKO06_04690"
     CDS             <1..2585
                     /locus_tag="PKO06_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV68974.1"
                     /translation="LRNLADRELKPSFERIPGVANVEVFGGLEREVRVKVSPERLRSL
                     LLTIDDIIHAVSRDNQNTPVGNITEGNFKYLIRSEGEFQKPEDLGNIIVKQIGQRPIY
                     LSDVAIISDSYKDIESVARLNGKPAVNLQLKKESGANPVQISDNVKKLIPRLESRYKG
                     RMKIAIAKDGSDFIRDSIQMVIDNATTGAILSIIVIFLFLWNVRSTIIIGTTIPMAVI
                     ITFAFMYLRPGMTLNLMTLGGLALGIGMMVDNAIVVLENIYRRMRFEGDDKRAEISVT
                     GTEQVTLAIFASTLTTVAVFLPIGFVPGVVGEIFNNMSLAIVFSLIASYLTAVTVVPL
                     MSSRMLRTKAEGREYIMDVFRGIYRAFLGFLLRSAWRRWAYFLLMVGLFVGSFRFFPP
                     MEFFPKMDRGTLILKYEMPEGTSIETTDLFSLKVEEIVRRFPEVERFQCNTKMAEGNV
                     TITLVPLEKRTKTTNQLVQLIRQEVARLPGYTDLTFAEPGMGPPDAGKPVQIEVSGDD
                     FDVVASLCLDITERVKNVPGLKDINSGVKAGRPEVRVEFDREKLKDLGIDLAGAAGML
                     RSFIYGTIAGKYKERNEEFDVRVEAMERYKDQVGKLEHLDMALDDKVVVNLAQIARFR
                     ESRGFSKIERKNLKRQLKVTADIENRPLQAVILDIQKLLKDVPVPSGYEINFGGEEEE
                     RAEAFGNLNWALLASIILVYMIMAAQFESLAQAFIIMFTIPLSIIGVILALNIYGFAF
                     SITAMIGIIMLAGIVVNNGIILIDYINQARAEGMEKIDAALESGAIRLRPVLMTVGTT
                     VIGMFPLSLGIGAGSDFYQPLAITVIGGLIVSTFLTLTYVPVVYVMVDNLVEGLTRIV
                     RRFI"
     gene            complement(2594..3004)
                     /locus_tag="PKO06_04695"
     CDS             complement(2594..3004)
                     /locus_tag="PKO06_04695"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011119515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNV68975.1"
                     /translation="MSVSIGIEYLGNLQCRAIHEPSQQALLTDAPVDNGGRGAEFSPT
                     DLVATALGTCLLTVMGLVAARSNIDLRGTTVQVTKEMTADPVRRIGRLEVQIRYPATL
                     VVPEVERLKLERAAALCPVKQSLHPDVEIVVSYL"
     gene            complement(3015..3608)
                     /locus_tag="PKO06_04700"
     CDS             complement(3015..3608)
                     /locus_tag="PKO06_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNV68976.1"
                     /translation="MGFVRRLYDWMLSWAEHPAGVWALFVLAFMESSFFPIPPDVLLI
                     ALAIGNPSRSLWFASVCTIGSVIGALVGYGIGWGVWEAVDQYFFAYVPGFTPELFKKV
                     CEQYELYSFWIVFTAAFTPIPYKVITISAGVSQISLLPFIVASIIGRGARFYLVAGLI
                     WKFGAPIKEFIDKYLNLLTILLTILGIGGFIALKFLF"
     gene            complement(3720..4739)
                     /locus_tag="PKO06_04705"
     CDS             complement(3720..4739)
                     /locus_tag="PKO06_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNV68977.1"
                     /translation="MHKQCWLVRAAVVLLLAPLGFSTPGYAQGSDASEQIPPPAISFA
                     REAPDASREKVPTPDPTAVYKNLVQLLETLEEKYPRDRAAFVQVQKHAYELMEQARRR
                     QVEATTPLPQALQMRKESRERNRGRVGPVDFVPVNMYAQCQSLRDEQLVRALRDLVDN
                     QIPVGYQAAQDIVFSKLDNKDGKVECVYTGRTIQTQGEPNASDMNIEHTWPQSQGATG
                     DAKCDLHHLFPTDSKANNTRSSFPFGNVENPSWTQGGSKFNGKVFEVRPQQKGNTARA
                     MFYFSMRYNKSIAPAQEAVLRQWHKGDPVDDEEIERNNRIENFQHNRNPFVDHPEFVD
                     QIEDF"
     gene            complement(4930..>5124)
                     /locus_tag="PKO06_04710"
     CDS             complement(4930..>5124)
                     /locus_tag="PKO06_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease domain-containing protein 2"
                     /protein_id="HNV68978.1"
                     /translation="GVKSAVALVLNRNFQKSKQVTTSNWAVQQLSPRQACYAANDAYA
                     ALQVWNALGRPGPEKKPRRR"
BASE COUNT         1130 a         1466 c         1448 g         1080 t
ORIGIN      
        1 ccctgcgtaa tctcgccgat cgtgaattga agccctcgtt cgagcgcatt cccggagtgg
       61 ccaacgtcga agtgttcggc ggactggaac gcgaagtccg agtcaaagtg tcaccagagc
      121 gtcttcgttc gctgctttta accattgacg atatcattca cgccgtcagt cgcgacaacc
      181 agaacactcc ggtcggcaac atcaccgaag ggaacttcaa gtatctcatc cgctccgagg
      241 gcgagtttca gaaaccagaa gacctgggca acatcatcgt caaacagatc gggcaacgac
      301 cgatctacct gagcgacgtc gcaatcatct ccgattcgta caaggacatc gaatcggtcg
      361 cgcgcctgaa cggcaagccg gcggtcaacc tgcagctgaa aaaggaatcc ggtgccaacc
      421 cggtgcagat ctcagacaac gtcaagaagc tgattcccag actcgagagt cggtacaaag
      481 gccgcatgaa gatcgccatc gccaaagacg gctcagactt catccgcgat tccattcaga
      541 tggtcatcga caacgcgacg accggcgcga ttctctcgat catcgtgatc ttcctgttcc
      601 tgtggaacgt gcgttcgacc atcatcatcg ggaccaccat tccgatggcg gtcatcatca
      661 ccttcgcttt catgtatctg cgccccggca tgaccctgaa tctgatgacc ctgggtggct
      721 tggcgctcgg tatcggcatg atggttgaca atgccatcgt ggtgctcgaa aacatctacc
      781 gtcgcatgcg tttcgaaggt gatgacaagc gcgcggagat ctccgtgacc ggaaccgagc
      841 aggtgacgct ggccatcttc gcgtcgaccc tcacaacggt tgcggtgttc ctgcccatcg
      901 ggttcgtccc cggggtggtc ggcgaaatct tcaacaacat gtcgctcgcg atcgtctttt
      961 cgctcatcgc ttcgtatctg accgcggtca cggtcgtacc cctgatgtct tcccgcatgc
     1021 tgcgcaccaa agcggaaggg cgcgaataca tcatggacgt attccgcggc atctaccggg
     1081 cattcctcgg gtttctgctt cgttccgcct ggcgccgatg ggcttacttt ctgctcatgg
     1141 tagggttgtt cgtcggttcc ttccgtttct ttccaccgat ggaattcttt ccgaaaatgg
     1201 accgtggcac actcatcctg aagtatgaga tgcccgaggg aacctcgatc gaaaccaccg
     1261 atctgttctc gctgaaagtc gaggaaatcg tgcgccgttt tccggaagtc gaacgatttc
     1321 agtgcaacac caagatggct gaaggcaatg taacgatcac actggttccg ctcgagaagc
     1381 gcacaaagac aaccaatcag ctggtacaac tgatacgaca ggaagtggct cgtctgccgg
     1441 gatacacgga cctgaccttt gccgaacccg gaatgggtcc gcctgacgcg ggcaagccgg
     1501 tacaaatcga agtctcgggt gacgacttcg atgtcgtcgc cagtctgtgt ctggatatca
     1561 ccgaacgcgt gaagaacgta ccaggactca aggacatcaa cagcggtgtc aaggccggtc
     1621 ggcccgaggt ccgggtcgag ttcgatcgcg aaaagctcaa ggatctgggg atcgatctgg
     1681 ccggcgcggc cggcatgctg cgctcgttca tctacggtac gattgccggc aagtacaaag
     1741 agcgcaacga agaattcgac gtgcgcgttg aagccatgga acggtataaa gatcaggtgg
     1801 ggaagcttga acatcttgac atggcgctcg acgacaaggt cgtggtcaat ctggcacaga
     1861 tcgcccgctt ccgggaatca cgcgggttca gcaagatcga gcgcaagaac ctgaagcgac
     1921 agctgaaggt gactgccgac atcgagaacc gcccgctgca ggcggtgatt ctcgacattc
     1981 agaaactgct gaaggatgtt ccggttcctt ccggatatga aatcaatttc ggcggtgaag
     2041 aggaagaacg cgccgaagcc ttcggcaacc tcaactgggc gctgctggcg tcgatcattc
     2101 tcgtgtacat gatcatggcg gcacagtttg aatcactggc acaggctttc atcatcatgt
     2161 tcacgattcc actgtcgatc atcggcgtga ttctggcact gaatatctac gggtttgcgt
     2221 tctcgatcac ggcgatgatc ggcatcatca tgctggcggg catcgtcgtg aacaacggca
     2281 tcattctcat cgactacatc aatcaggcgc gggcggaagg catggagaag atcgatgccg
     2341 cgctcgaatc aggggccatt cgtctgcgac cggttctcat gacggtggga acgacggtca
     2401 tcgggatgtt tccgctgtcg ctcggaatcg gtgccggatc agacttctac cagcctctgg
     2461 cgatcacggt catcggcggc ctgatcgtct cgacctttct gaccctgacg tatgtaccag
     2521 tcgtctatgt catggtcgac aatctggtcg aaggcctgac ccgcatcgtc agacgcttta
     2581 tctgaccctc agttcagaga tagctcacca cgatctccac atccgggtgc aggctctgct
     2641 tgaccgggca aagggccgcg gcccgttcga gcttcagccg ttcgacttcg ggtacaacca
     2701 gcgtcgccgg atatcgaatc tgcacttcca gccttccgat tctcctcacc ggatcagcgg
     2761 tcatttcttt tgtgacctgt acggtcgtcc cgcgcaaatc gatattggag cgtgccgcaa
     2821 ccagccccat gacggttaac agacaggtac ccagggccgt agcgacaagg tcggtcggag
     2881 aaaactcagc gccccgacca ccgttgtcga cgggggcatc ggtcagcaac gcctgctgcg
     2941 atggctcatg aatcgcacga cactgcaggt tgccgagata ttcgattcca atggagacgc
     3001 tcacgacgac actcctagaa caggaatttg agggcgatga agccgccaat gccgaggatg
     3061 gtcagcagga tggtcagcag gttcagatac ttgtcgatga actccttgat cggtgctccg
     3121 aacttccaga tcaggccggc gaccagatag aaacgcgcgc cacgtccaat gatgctcgcc
     3181 acgataaacg gcaggaggct gatctgcgag accccggcgc tgatggtgat gactttatac
     3241 ggaataggcg taaatgctgc cgtgaagacg atccagaatg aatataactc gtactgttcg
     3301 caaaccttct tgaacaactc cggagtaaac cccggcacat aggcaaagaa gtactgatcg
     3361 acggcctccc agacgcccca gccgatccca taaccgacca gagcgccaat cacagagccg
     3421 atggtgcaga cagaggcaaa ccagagagag cgcgacggat tgccgatggc cagtgcaatg
     3481 agcaggacat cgggcggaat cgggaagaaa ctcgattcca tgaacgccag cacgaacaat
     3541 gcccagaccc cagccggatg ctcggcccac gagagcatcc aatcatacag acggcggacg
     3601 aatcccatga gtaactcctg gtgtgacggt ctcgtcgttc agcaggcggc aagtataccg
     3661 gcatttcacc caaaaagaaa ccggcgctcc agagagcgcc ggttgtgcaa gctgagaaat
     3721 cagaaatctt cgatctgatc aacgaactcg gggtgatcca cgaacggatt tcggttgtgc
     3781 tggaagtttt cgatgcggtt gttgcgttcg atctcttcgt catcgaccgg atcgcccttg
     3841 tgccactggc gcagcacggc ttcctgggcg ggggcgatgc tcttgttata gcgcatcgag
     3901 aagtagaaca tggcgcgggc ggtgttgccc ttctgctgcg ggcgcacctc aaagaccttt
     3961 ccgttgaact tgctgccgcc ctgagtccag ctggggttct cgacgttgcc gaacgggaaa
     4021 ctggaccgcg tgttgttcgc cttcgaatcg gtggggaaga gatgatgcag atcgcacttg
     4081 gcatcacccg tcgcgccctg actttgcggc caggtgtgct cgatgttcat gtcagaagca
     4141 ttgggctcac cctgggtctg aatcgtgcga ccggtataga cgcattccac cttgccgtct
     4201 ttgttgtcga gcttcgaaaa aacgatatcc tgagcggcct gatagccgac ggggatctga
     4261 ttgtcgacca ggtcgcgcag agcccgaacc agctgttcgt cgcgcagact ctggcactgg
     4321 gcatacatgt tgaccggcac gaaatcaacc ggaccgaccc ggccgcgatt acgctcacgc
     4381 gactctttcc gcatctggag tgcctgtggc agaggagtgg tcgcttcaac ctggcgccgg
     4441 cgagcctgtt ccatcagttc atatgcgtgc ttctgcacct gcacgaaggc ggcgcggtcg
     4501 cgcggatact tttcttcgag agtttcgaga agctgcacga gattcttgta gactgccgtt
     4561 ggatcaggtg tcggaacctt ctcgcgactg gcgtcggggg cttcacgggc aaacgaaatg
     4621 gcaggtggcg gaatctgctc ggaagcgtcg cttccctggg cgtagcctgg agttgaaaag
     4681 ccaagcggcg ccaacaatag aaccacggct gcccgaacca accagcattg cttatgcata
     4741 ctctattctc cctgatctgc tgcgttcgtg ttttgtaagt atacacacag aagacagggt
     4801 accggaagcc ccttcaagaa acttttttcg tggctgcgct ctgtcgctca ccagggtcat
     4861 gcgaaagtag cggaacagcc gtgttccgag ttgctcagac tggagttttt tgagtgagac
     4921 tcgacgaagt cagcggcgac gcggtttttt ttctggacca ggcctgccga gggcgttcca
     4981 gacctgcaat gcggcatacg cgtcattggc cgcataacag gcctgacgcg gcgacagctg
     5041 ttgcaccgcc cagttcgagg tcgtcacctg cttcgatttc tgaaagttgc gattgagaac
     5101 cagcgccacc gctgacttca cccc
//
LOCUS       DAORHD010000157         5116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_16990_length_5116_cov_3.194033, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000157 DAORHD010000000
VERSION     DAORHD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16990_length_5116_cov_3.194033"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKO06_04715"
     CDS             complement(<1..784)
                     /locus_tag="PKO06_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68979.1"
                     /translation="MMFLRELARISNVVLIVTIMIGTGWVGKTARLEAASGKNDSLKP
                     LVRAIFGQLSQASSATETTALPEGMHLIKFTGCTGLCLEPGKFHLRPEIWTDYEGLMD
                     RFHKAQGRKPAPPVAAINGLFYSASGIVGQILVDGRIPPRFQQMHGSKPRCFLAVLKQ
                     GEKQRWVIGETTLPLKDFEAEGALGLTRFNRPWKAGETLYQMMHGGGWIIRDGRDVHM
                     ESYKKQGFRFRKVDQDSRHTVIAMDRQDRLYLLVFEAGANLEA"
     gene            complement(793..3687)
                     /locus_tag="PKO06_04720"
     CDS             complement(793..3687)
                     /locus_tag="PKO06_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68980.1"
                     /translation="MKTHFFHHRLTHRLLVGVCLLFCWSLVVSGSVLAVELDTVYRLL
                     GEYRVSTAPGLRVYYPVTCQPVLSRVMETFSRVRDRYKEIFPELKDYPVSVLLTDHDD
                     RDASKSDATFDLITLSLVEEVGALSTRGYSLEQRFSLRLASIMVLRSLGSSHVALKRR
                     LALLSIPPWFLEGLAMHYAFPMDALHNARLLELARTRSLFALDDLDTIQDQSMIVQEE
                     MRFQARQMIDYWHDISTSTAGLRFLDLIKNRPAGFDQTFRDAFGFPLKDGLRRYNDWM
                     RHLCEQRGCSEQRLPETIPGFRGQHYQQGVHSLPNGGYAWVSSGRYHDEVYDLWLGVP
                     GKARVALKNVHPALWLDTASQTIYIGKYEVNQKRQRRLKLWQVPLKGRPRRLASEDGS
                     HRPLGMYQDRLLYVRTQSGNTKVMSVDLSASGTIRLEFGFPEAWRPLEIAWDQAGETL
                     LFTMQNNSTNRLCRVRPSQGPESAEVIFEQEGSINRLQSRQGKIFVSCESPSQFSQIY
                     RLEKASGTFDCQPVALNGTEVWDTTVASETFVSRRVTEVPGGVWDFSVASDTLLVTTV
                     RGRGFLPVRLPLPSKDDAQPVALTASPDANLERTADVGTATTYVPTALGVASSTEVAW
                     KPYASEFRSSYWLPKITRDDQGAVGGIYSYRADRLDRQRFIISPTYGFKSKDWGYLAD
                     YQRRWGLFSAGFTMQDRVQRKSYLSNSYYERVHSADLHFEYPFSLSTSVTVGGNLSKR
                     AIAEFPDNPTASVPTVGRDNSIYINVKQRSIRTEPLWEVFPRRGREIDVAYRKGLDMF
                     DGELKYDSMSVRWNEYVPLPAGFVGTLRLWFAEDDKEGGIRRPDDLSLGGSEYLRGFP
                     GSVRFGDSLRYTGVHIGHPINFEIPWLRRWVQKEIIVLEGFAERGDVRTEGRSFDYLS
                     DYGVEIRAKGLLLRRLPVTVRMGQAWPRHGDGKSHSYWTVDFSTLSSLIK"
     gene            complement(3847..>5116)
                     /locus_tag="PKO06_04725"
     CDS             complement(3847..>5116)
                     /locus_tag="PKO06_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68981.1"
                     /translation="RQKGMTDEMFGEFMAAVLSSEIHDTDKIAEYISECKAMGIEMLA
                     PDINRGKKLFTVESRSGNGGGKAIRFGLVAIKGVGDSVVEAIIAAREKGGEFKNLLDL
                     TRRVDRKIINSKAVESLVKGGAFDAWGINRGVLLAMVPDALKLGAQSQSEKETGQATF
                     FDLLGNESDSFGATEMPLPKVPEMSRRDVLAAEKEALGFYFSGNPFAEVAPVGRLFAN
                     LTIKDLIERTPSDPVRLTGILLTMKKHTTKKGDLMAFMMIEDDTASLDVTMFPSMYAK
                     IAMNLKVDQPLFLLVRAEPPAGETDKPRVSIEELYFEEDLNKDGFVNLDLVIPLGANN
                     PDNLKKLHSIVRRYPGTTPFSLIVSTPSGERVRLKPPTHLRASFCSGLVREWEAICGA
                     RTVLAQFPEVGKASARNNNRNQYRNRQAAG"
BASE COUNT         1053 a         1553 c         1480 g         1030 t
ORIGIN      
        1 ccgcctcgag attggcaccg gcttcgaaga ccagcagata caggcggtcc tggcggtcca
       61 tggcgatcac ggtgtgccga ctgtcctgat cgaccttacg gaagcgaaag ccttgtttct
      121 tgtacgattc catgtgtaca tcgcgaccat cacgaatgat ccagccacca ccgtgcatca
      181 tctgatacag tgtctcgccg gctttccagg gccggttgaa ccgcgtgaga ccgagagcgc
      241 cttccgcttc gaagtctttg agcggcagcg tggtttcgcc gatcacccag cgctgtttct
      301 cgccctgttt cagcaccgcc aggaagcagc gaggcttgct gccatgcatc tgctgaaatc
      361 gcggcggaat tctgccatcg accagaatct gccccacgat acccgacgcg gaataaaaca
      421 gtccattgat agcggcgacg ggcggcgcag gcttgcgccc ctgcgccttg tggaagcggt
      481 ccatgagccc ttcgtagtcg gtccagatct cgggtctgag gtggaatttg ccgggttcga
      541 ggcacagacc ggtacacccg gtgaatttga tcagatgcat gccttcgggc aacgcggtcg
      601 tttcggttgc tgacgaggcc tgcgacaact ggccgaatat cgcccgcacc agcggcttga
      661 gagaatcatt ctttcctgag gcggcttcca atctcgctgt cttgcccacc caacccgtac
      721 cgatcatgat cgtcacgatc agcacgacgt tcgaaattcg ggccagctca cgcagaaaca
      781 tcataacgac tcctatttga tcaagctact cagtgtggaa aaatcaacgg tccaataact
      841 gtgactcttg ccgtcgccgt gtcgcggcca cgcctggccc atacgcacgg tcacgggcag
      901 acggcgcagc agcagcccct tggcgcgtat ttcgaccccg tagtccgaga gatagtcgaa
      961 cgaacgtccc tcggtgcgca cgtcaccgcg ctcagcaaag ccttcgagca cgatgatttc
     1021 tttctgaacc cagcggcgca gccacggaat ttcaaagttg atcggatgtc cgatgtgcac
     1081 tccggtatat cgcaacgaat caccaaaacg caccgacccg ggaaacccgc gcaggtattc
     1141 cgacccgcca aggctcagat catccgggcg gcgaatgccc ccttccttgt cgtcttcggc
     1201 aaaccacagg cgcagcgtgc cgacgaaccc ggccggcaac ggcacgtatt cgttccagcg
     1261 taccgacatc gagtcgtatt tcaactcgcc gtcgaacata tcgaggccct tgcggtaggc
     1321 cacgtcgatt tcgcgcccac ggcgcgggaa gacttcccac aggggttcgg tgcgaatcga
     1381 acgttgcttg acgttgatgt agattgagtt gtcacgacca acggttggga cagaagcggt
     1441 cgggttgtcg gggaactcgg caatcgcccg tttcgagagg ttgccgccga cggtcaccga
     1501 cgtggaaaga ctgaacgggt attcgaaatg cagatctgcc gagtggacgc gctcatagta
     1561 actgttcgac agatagctct tgcgctgcac gcgatcctgc atcgtgaacc cggccgaaaa
     1621 aagcccccag cggcgttgat agtccgccag gtatccccag tctttactct tgaacccata
     1681 ggtgggcgaa atgataaacc gctggcggtc gaggcggtcg gcccgatagc tgtagatgcc
     1741 gccgacggcc ccctggtcat cgcgcgtaat cttgggcagc caataggaac tgcgaaactc
     1801 ggacgcatac ggcttccatg caacctccgt ggaagaagcg acaccgaggg cggtcggcac
     1861 gtacgtcgtc gccgttccca cgtcggcggt tcgttcgagg ttcgcatcgg gactggctgt
     1921 cagcgcaacc ggttgcgcat cgtctttgga cggcaagggc aggcgcacgg gcaggaatcc
     1981 gcgcccacgc acggtggtca ccagcagggt gtccgaggca accgagaagt cccagactcc
     2041 cccgggcact tcggtcacac ggcgggaaac gaaggtctca gaagcaacgg tcgtatccca
     2101 gacttccgtg ccgttgagag cgacaggctg acagtcgaac gtccccgaag ctttttccag
     2161 acgatagatc tgcgagaact ggctcggact ttcacaggac acgaaaatct ttccctgcct
     2221 ggattgcaga cggttgatgc tgccttcctg ctcgaagatc acttccgccg attccgggcc
     2281 ttgcgacgga cgcacgcggc agagccggtt cgtcgaattg ttctgcatgg tgaacagcag
     2341 ggtctcgccg gcttgatccc aggcaatttc gagcgggcgc caggcttcgg gaaaaccgaa
     2401 ctcgagccgg atcgtgccgc tcgcgctgag atcaaccgac atcaccttgg tatttccgct
     2461 ttgggtgcgc acgtagagca ggcgatcctg gtacatcccc agggggcgat ggcttccatc
     2521 ttcactcgcg aggcgtcgcg gtcggccctt cagcggtacc tgccacagct tgagtcggcg
     2581 ctgccttttc tgatttacct cgtatttccc gatatagatg gtttgagaag cggtatcgag
     2641 ccagagagcc ggatgcacgt tcttcagcgc cacacgcgcc tttccgggaa cgccaagcca
     2701 gaggtcgtag acctcgtcgt ggtatctgcc cgatgagacc caggcatacc ccccgttggg
     2761 cagggaatgc actccctgct gatagtgctg tccacggaac ccgggaatcg tctcgggaag
     2821 cctctgttcg gagcagccgc gttgttcgca gaggtgtcgc atccagtcgt tgtaccggcg
     2881 caaaccatcc ttcagcggga atccaaaggc atcgcggaac gtctgatcga agcccgcggg
     2941 tcgattcttg atcagatcga ggaaacgcag tcccgccgtc gacgtggaga tgtcatgcca
     3001 gtagtcgatc atctgacgcg cctgaaagcg catttcctcc tggacaatca tgctctgatc
     3061 ctgaatggtg tcgagatcgt caagtgcaaa gagactgcgg gtgcgggcca gttcgagcag
     3121 acgcgcgttg tgcagcgcat ccatggggaa ggcgtagtgc atcgccagtc cttccagaaa
     3181 ccagggcgga atcgacaaca gcgccagacg gcgcttcaat gccacatggg atgagcccag
     3241 agaacgcagc accatgatcg acgcgaggcg caatgaaaat cgctgttcga gcgagtagcc
     3301 gcgtgtcgag agcgcgccca cctcttcgac gaggctcagc gtgatcagat cgaacgtcgc
     3361 atctgactta ctggcgtcac ggtcatcgtg atcggtgagc agcaccgata ccgggtagtc
     3421 cttcagttcg ggaaagatct ctttgtaccg gtcacgaacc cgcgaaaatg tctccatgac
     3481 ccgactcagc acgggctggc aggtgaccgg atagtagacg cgcaaaccgg gggcggtcga
     3541 aacccggtat tctccgagca gtcggtagac ggtgtcgagt tcaacggcca acacggaccc
     3601 ggaaaccacc agtgaccagc agaacagcag acacacgccg accagaagcc tgtgtgtcag
     3661 acgatgatgg aaaaagtgcg tcttcatgct ttcgcctctc gaacccgatt cggtcagcat
     3721 attaccgcag agctcgccgg aatgcgaaca aaaaggcgca gaggcaggtc aaatgcatcc
     3781 ttttcctgcg tggtctcggg ctttgcggct caccctcgtc agaccgctcc ataccctcgg
     3841 cgaggatcag cctgcggcct ggcggttgcg atactggttg cggttgttgt tgcgggccga
     3901 agccttgccg acttcgggaa actgcgccag aaccgtgcga gctccgcaga tggcttccca
     3961 ttcgcggacc agccccgagc agaatgaggc gcgcaagtgt gtcggcggct tcagtcgaac
     4021 ccgttcaccc gaaggcgtcg agacgatcag actgaacggc gtcgtgccgg gatagcgacg
     4081 cacaattgag tgcagcttct tgaggttgtc cgggttgttg gccccgagag gaatgaccag
     4141 atcgaggttt acgaacccat ctttgttcag atcttcctcg aaatacagtt cttcaatgct
     4201 gactcgaggc ttatccgtct caccggccgg tggctcggct cgcacgagca gaaagagcgg
     4261 ctgatcgact ttcagattca tggcgatctt ggcatacatc gacgggaaca tggtgacatc
     4321 gagactcgcc gtatcgtcct cgatcatcat gaatgccatc agatcgccct tcttcgtcgt
     4381 gtgcttcttc atcgtgagca gaatgcccgt gagccgcacc gggtctgaag gcgtgcgctc
     4441 gatcagatct ttaatcgtca ggttcgcgaa caaccgaccg acgggcgcaa cttccgcgaa
     4501 cggattcccg gaaaaataga accccagcgc ctctttctcg gcggccagca catcgcggcg
     4561 actcatctcc ggcactttgg gcagtggcat ctcggtagcg ccaaagctgt ctgactcgtt
     4621 gcccagcaga tcgaaaaacg tggcctggcc ggtctccttc tcgctctgag actgtgcgcc
     4681 aagtttgagc gcatcgggaa ccatcgccag cagcacccca cggttgatgc cccacgcgtc
     4741 gaacgcgccc cctttgacga gcgattcgac ggccttcgag ttgatgatct tacgatcgac
     4801 gcggcgagtc agatcaagca ggttcttgaa ttctcctcct ttctcacgcg cggcgatgat
     4861 cgcctcgacg accgaatctc cgactccctt gatcgcgacg agcccgaagc gaatcgcctt
     4921 gccgccgcca ttcccggacc tggattcgac ggtgaagagc tttttccctc tgttgatgtc
     4981 gggggccagc atctcgatgc ccatggcttt gcattcgctg atatactcag cgatcttgtc
     5041 ggtgtcgtga atttctgagg aaagcacggc ggccatgaac tcgccgaaca tctcgtcggt
     5101 catgcccttc tgtcgc
//
LOCUS       DAORHD010000158         5114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17000_length_5114_cov_3.825064, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000158 DAORHD010000000
VERSION     DAORHD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17000_length_5114_cov_3.825064"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1179
                     /locus_tag="PKO06_04730"
     CDS             166..1179
                     /locus_tag="PKO06_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68982.1"
                     /translation="MSAIINSQGGQMETSGGKLGGLGALGFFVAWAIGSYLFLAVMGY
                     GFITELPMLFSPLSKVNQLIYLQRFYSRLDDERGVLRTTQRAWRMTLSNMKDGVIPDL
                     HLQTSQGVLKVPVRAFQQFLEEARIPLDKPSNQIPAWKRQARAGVEWAVLPDFYGFPL
                     VPIGSEDLLARHFPDLRDARSLRVLFDVSIDPATSVKSRLQEVGTIDSFFTYPLIWRA
                     QKFVEKVQRKRSGALIWVVVQVIGAFVTLWLLTRPVPPLYAEPLRFLAGAFGVAAIFG
                     FLFRFYATCYMAARISDDQTIDGATLELIRTDCQKYVDDLQQEQRISPEDRKILSELL
                     RRG"
     gene            complement(1156..3630)
                     /locus_tag="PKO06_04735"
     CDS             complement(1156..3630)
                     /locus_tag="PKO06_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68983.1"
                     /translation="MKDQRITGLSARSLWFGWIITLLIPGIGLFLFLPSWIEREESQL
                     LQAYQRLLVSDMRDLQRQLFLYDHSSRQEQDLRQQLEHALASTSTSWHGCARQLLLNE
                     DQLFLPGFLQTAAGMAYRDFLPAEFYHLILPRHASSTGSLHMNRDAPVLCLETSIPLS
                     QLVHPRRSLFEVVWLFLLIALLLSAVIILLRHRIPLPFFSLRLKTGAMIGLGLFLPIT
                     GFWLATISRYDTQNLLQKMELTHYLEHQLESLEYRLASDEDQMARKLLRLIDSHSVSL
                     AKPESAKSALEQIMSQWPTGVLCLFRSDRTYVEVEPKQERYLLPEHQGLREGVRRIMS
                     TAIRPLFSAPVSQTTTDKTPSPEEVFLQELLETVVPSKRIHEIYRNPGILFQGFETLS
                     FTRTLFQPLASHTLILEMTGKGEFFWVPFLNRLLRGIPGEPLSQLREEIESTTIEYGV
                     FLKPYRHALSFERLWLPIGIEDRQTFIQAAHQNLVEGNSRLTDSWSDDTPSLLFIRPF
                     SKLPIVGIARATPRPGSLPTHRLAGLLTGIYALALLLFLAGVLTRLTSAPIALFIQAV
                     ERVQDGDYQTRLNMPTGDEIEEIGGTFNAMVEALDQKRRMSRFVSENVMEAIKEDNAG
                     EGRREIATVLFCHVRDFEEMLLSSAPEEIFHFLNLYFTQMGFVIRRQGGTIDKFIGDA
                     VMAVFQDRGSQPITEPAPVRAARAALLMQEQLANMNTFRRERQQSSVSIGIGIHHGEV
                     IAGRIGSQHGRLDFTVIGDTVNLAARLESQSRLASETGILISSSVEASLPIDIQRRPL
                     GQITVKGRAESVTVFTLSGSTPTQKL"
     gene            complement(3627..>5114)
                     /locus_tag="PKO06_04740"
     CDS             complement(3627..>5114)
                     /locus_tag="PKO06_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV68984.1"
                     /translation="QKFQMETQAAMAGGLRTYLKDRQVYHETFIYPPGHKPPAKSIVS
                     PATDFGKASVVELRQGSFLRTVYPNLEEVFFTFLILPASSTPTLPRATCAMLIDPREI
                     VRQYLQELQAAPDQLLNQEADFRTEYRMYLYDPLQKRLLQGTDPNLAENDFTLIAQRT
                     INAGQGVTQNLLHSPRSRLVQSRIFHGFPGVAVAIGTPVQSTSAGISASTFLIVLILF
                     SLLIVLLMSRFLSEILVEPFQVFQHGVTRIARGEYGTGIFLDTRDEFQQLGQSFNQLS
                     EELAQKERMKRFLSERIIRAVECDAPGPPQQIQEASILVSDLRGFTTLSERHPPEQIT
                     AMLNSYFTTMEEAIQEEGGFIDSFIGDCLVAVFLPVADATTPAHRSLRAGIAMRRRLA
                     EFNRERRQRGEFLIDNGVGIAIGPIITGLIKSGFKRRELGILGEPVREAHRLETQTRK
                     ARCSGIVVSQRLQDQLAATWTFTPLPDSRDAWELSLDLPVEGRTR"
BASE COUNT         1146 a         1297 c         1564 g         1107 t
ORIGIN      
        1 gcatgaaaaa ggtttgaacg cgtctcgcca acgtgtgcaa cagtgccgag gtaggcaaga
       61 aaccccttct ttcaggtcaa gatatgaaag ccgctcccca agcttgtact tgaattgttc
      121 ttcgggtgca acaggtcttg agcggctctg tttgcctctg ttatcatgtc tgccatcatc
      181 aactctcagg gcgggcagat ggaaacatcc ggtggcaaat tgggcggtct tggcgctctc
      241 ggcttttttg tggcgtgggc catcggttcg tatctctttc tcgcggtcat gggctacggc
      301 ttcatcacag aactgccgat gctgttcagc ccactgtcaa aggtgaatca gctcatctat
      361 ctgcaaaggt tttactcccg gcttgatgac gaaagaggtg ttctcaggac aacgcagcgt
      421 gcctggagaa tgaccctgtc caacatgaag gacggagtca ttccagatct tcacctgcag
      481 acatctcagg gggtcctgaa ggtgccggtg agagcctttc aacagtttct cgaggaggcg
      541 agaatccctc tggataaacc atcgaatcag attcctgcct ggaaaagaca ggcgcgtgct
      601 ggggtagaat gggctgtcct gccagacttt tacggttttc cactggtccc gattggttca
      661 gaagatctgt tggcccggca tttcccggat ttgcgcgatg cgaggtcatt gcgggtgctg
      721 tttgacgtgt cgatcgatcc tgccacatcc gtgaagtcga gactgcagga agtggggacg
      781 atcgactcat ttttcactta tccgctgatc tggcgagcgc agaagtttgt cgaaaaagtg
      841 cagcgtaaac gttcgggagc actgatctgg gttgtcgtac aagtgatcgg ggcgtttgtg
      901 actctctggc tgctgactcg accagttcct ccgctgtatg cggaacccct tcgctttctg
      961 gctggggcat tcggtgtggc ggcgatcttt ggatttctct ttcgtttcta tgcgacgtgc
     1021 tatatggctg cacgcatcag tgatgatcag acaatcgatg gcgccactct ggaactcatc
     1081 cgtacggatt gtcagaagta cgtcgacgat ctgcagcagg aacaacggat ttcacctgaa
     1141 gatcgaaaga tcctgtcaga gcttctgcgt cggggttgag ccggagagcg tgaagacggt
     1201 caccgattcg gcgcgtcctt tgactgttat ttgtccgagt ggacgtcgct gaatatctat
     1261 tggcagagac gcttctacgg aagatgaaat cagaattccg gtttcgctcg cgagacgtga
     1321 ctgtgattcg aggcgcgcgg caagattgac cgtgtctccg atcaccgtga aatcgagtcg
     1381 tccgtgctga gagccgatgc gtcccgcgat gacctcgccg tgatgaatgc cgataccgat
     1441 cgacacggaa gactgctgcc gttctcgccg aaaggtgttc atattggcta actgttcctg
     1501 catcaagagt gccgctcgcg cggcgcgcac gggagccggc tcggtaattg gctgagagcc
     1561 gcgatcctga aatacggcca tgacggcatc accgatgaat ttgtcgatag tcccaccctg
     1621 acgtcgaatg acgaagccca tttgggtaaa gtacaggtta agaaagtgaa agatctcttc
     1681 cggtgcagaa gaaagcagca tctcttcgaa gtcgcggacg tgacaaaaga ggacggttgc
     1741 gatttccctg cgcccttctc ccgcgttgtc ctccttgatc gcttccatga cgttttcaga
     1801 cacaaaacgc gacatgcgtc gtttctgatc gagagcttcg accatggcgt tgaaggtgcc
     1861 gccaatctct tcgatctcgt ctcctgtcgg catgttgaga cgggtttgat agtcgccatc
     1921 ctgtactcgc tcgacggctt gaatgaacaa ggcaatcggg gcagacgtga gacgcgtcag
     1981 gacccccgcc aggaacaaca acaacgcgag cgcgtagatc ccggtcagga gtcccgcgag
     2041 gcgatgcgtg ggaagggacc cgggccgtgg agtggcgcgc gcgatgccga cgatggggag
     2101 tttgctgaag gggcggataa agagcagcga tggagtgtcg tcgctccagg aatcagtcag
     2161 gcgggagttc ccctcaacaa gattctgatg cgccgcctga atgaaggtct gacgatcctc
     2221 gatgccgatc ggaagccaga gccgttcgaa cgagagcgca tgccggtacg gtttcagaaa
     2281 tactccgtac tcgatggttg tcgattcgat ttcttctcgc agttgagaca ggggttcgcc
     2341 ggggatgcct cgcagcagac gattcagaaa gggaacccag aagaactcac ccttgccggt
     2401 catctcgagg atgagggtgt gagaggccag tggctgaaag agcgtccggg taaatgaaag
     2461 ggtttcaaag ccctggaaga ggataccagg attacgatag atttcatgaa tgcgtttcga
     2521 gggaacgacg gtctcgagca gttcctgaag aaacacctct tcaggagaag gagttttgtc
     2581 cgtcgtggtc tgggagacag gggcagaaaa caggggtcgg atagcggtgg acatgatgcg
     2641 tcgtacaccc tcacgcaggc cctggtgctc gggaagaaga tatcgctcct gcttcggctc
     2701 gacctcgaca tacgtacgat cagacctgaa gaggcagaga acccccgttg gccactggga
     2761 catgatctgt tccaaagccg actttgccga ctccggcttg gcgagtgata ccgagtgtga
     2821 atcgatcaga cgcaaaagtt ttctcgccat ctgatcttca tcgcttgcca accggtactc
     2881 cagcgattcg agctggtgct cgagatagtg tgtcagttcc atcttctgca gcagattctg
     2941 ggtgtcgtag cgagagatgg tcgccagcca gaatccggtg atcggcagga agagaccgag
     3001 accgatcatc gctccggtct tgaggcgaag agagaagaac ggcaggggta tccggtggcg
     3061 tagaagaatg atcacggctg acagcagtag tgcgatcaga agaaacaacc agacaacctc
     3121 gaacagagag cgccgcggat gtacgagttg tgaaagtgga atactggttt ccagacagag
     3181 gacgggtgca tctcgattca tgtgaagaga gccggtcgat gaggcgtgtc tgggaaggat
     3241 gagatgatag aactcggcgg ggagaaagtc gcgatacgcc atgccggctg ccgtttgcag
     3301 aaaaccgggc agaaagagct ggtcctcgtt gaggagcagt tgccgggcac aaccatgcca
     3361 tgaagtgctc gtcgaggcca gcgcgtgctc gagttgttgc cgcagatcct gctcctgacg
     3421 cgatgaatgg tcgtagagaa agagttgtcg ctgcagatcg cgcatatcac tcaccagaag
     3481 gcgctgatag gcttgtaaca actgggactc ttcgcgctcg atccaacttg gcagaaacag
     3541 aaacagcccg attcccggaa tcaggagcgt gatgatccag ccgaaccaca gactgcgtgc
     3601 ggagagaccg gtgatgcgct gatccttcat cgcgtgcgtc cctccacagg gagatcgaga
     3661 gacagttccc aggcatcgcg agaatccggg agcggtgtaa atgtccaggt tgctgccaac
     3721 tggtcctgca ggcgctgaga gacaacgatt cccgaacagc gcgcttttct cgtctgagtt
     3781 tcgaggcgat gagcctcgcg tacgggctca cccaggattc cgagttcgcg tcgtttgaac
     3841 ccggacttga tcaggccggt gatgataggg ccgatggcga tgccaacccc attgtcgatg
     3901 aggaactccc cgcgctgccg gcgttcccgg ttgaactcgg cgagtcgacg acgcatcgcg
     3961 atgccagcgc gcaaggaacg atgcgcgggt gtggtcgcat cagcgaccgg caaaaacacc
     4021 gcgaccagac agtcgccgat gaagctgtcg atgaagccgc cttcttcctg aatggcttct
     4081 tccatggtgg tgaaatacga gttcagcatc gcggtaatct gctcgggagg atgacgttcg
     4141 gagagagtcg tgaaaccgcg caggtcagag acgagaatgg acgcctcctg gatctgctgg
     4201 ggcgggccgg gagcatcgca ttcgacggct cgaatgatgc gctctgacag aaagcgcttc
     4261 atccgctctt tctgcgccag ttcttccgaa agttgattga acgactgtcc gagttgttga
     4321 aactcgtcac gggtgtcgag gaaaattcca gtgccgtact caccccgcgc aatccgcgta
     4381 accccgtgct ggaacacttg aaagggctcg acgagtatct cgctgagaaa ccgactcatc
     4441 aacagcacga tgagaagcga gaataagatg agaacgataa ggaacgtgga ggcggatatt
     4501 cctgccgaag tgctctgcac cggggtgccg atggcgaccg ccactccggg aaaaccgtga
     4561 aagatgcgtg attgtaccag ccgggatcgg ggagagtgca gaagattctg ggtgactccc
     4621 tggccggcgt tgatggttcg ctgggcgatg agggtgaagt cattctcggc gaggttgggg
     4681 tcggttcctt gtagaagtcg cttctggagc ggatcataca gatacatgcg gtattcggtg
     4741 cgaaaatccg cttcttgatt gagtaactga tcaggagcgg cctgaagctc ctgcaggtat
     4801 tgacgaacga tttctcgcgg atcgatcagc atggcacagg tggcccgggg aagtgtgggg
     4861 gtcgaagacg ccggcagaat cagaaaagtg aaaaacacct cttccaggtt cgggtagacg
     4921 gtgcgcagga atgacccctg acgcagttcg acaaccgaag ctttgccgaa atccgttgcc
     4981 ggagaaacaa tacttttcgc ggggggtttg tgccccggcg ggtagatgaa tgtctcgtgg
     5041 taaacctggc gatctttcag ataggttcgc agacctccgg ccatcgctgc ctgtgtttcc
     5101 atctggaact tttg
//
LOCUS       DAORHD010000159         5098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17094_length_5098_cov_3.338092, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000159 DAORHD010000000
VERSION     DAORHD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17094_length_5098_cov_3.338092"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PKO06_04745"
     CDS             <1..635
                     /locus_tag="PKO06_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68985.1"
                     /translation="TQQQVPYEWAELTDITWFDARMATIVGLGMALRTDDGGATWKQF
                     DQGFKPRATGIVRVSDTVGFLLGLDGNVMKTTNAGMSWRKVSVPGDGHPYGGWFVDDK
                     TGFLSGQTGIRVTYDGGESWSELAGSPDDTRVIIFTSPTTGYAFGRRYSGGCFGTHAP
                     HVSCTRDGGKTWTEQQTLRDLGGLCERLIQGEPGIWIGVAGGTVFKLKFL"
     gene            790..2523
                     /locus_tag="PKO06_04750"
     CDS             790..2523
                     /locus_tag="PKO06_04750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNV68986.1"
                     /translation="MLASRSHLVTYREDAADAEIPSHRLLLRGGYIVKVGAGLYVYSP
                     IMWRVLRKIERIVREELDRAGALEIQAPILQPVEYWQESGRLAVYKASGTMLFTKDRN
                     GGEFGLAPTAEEIVTAFVKATVQSYRQVPLTLYQIHTKMRDEIRPRFGLLRVKEFIMK
                     DAYSFDIDKAGMEKSYQAMKEAYFRIFSRIGLEALGVEADPGDIGGSGSMEFMIAANT
                     GEDTILIEEGTGYAANVEKAVSRLEPPVSANDALKPLEIRDTPNIRTCEQLRDFFDGF
                     AVERMVKTILYKLTYAEEEKYCAVLMRGNKEVNEVKLKNHFGCLAVRMLAGEEIQALT
                     GAEMGFAGPIGLNPKFHLLADESVRGMRNVICGVNQNHKHALHANIDRDFTPKEYVDV
                     RMAVVGDAGPIEGKPMIVKKGIEVGHIFQLGTKYSEPMQAKFLSKDGKQAVFHMGCYG
                     VGVSRLAAAAVEQTATDQGFVWPAAIAPFHVAITCLAGKDQAAVAATDQAYRDLQGRG
                     FDVLLDDRDLTVGVRLKDLELMGIPALVLIGKKWKESGEAEIKIRATGKTLFAKLEQL
                     PDVLKQEGCFA"
     gene            complement(2630..3772)
                     /locus_tag="PKO06_04755"
     CDS             complement(2630..3772)
                     /locus_tag="PKO06_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNV68987.1"
                     /translation="MFSEAERQTLFPITRTWSYLNHAAIGPLPTPVIEAAQTYLARCS
                     QQGEHHWEHTGQLLEHLRSRLAASLRCLADEVTFTRNVSEGISFLASGLDWSPGDNVI
                     LPAIEFPANVYPWLNQARNGVETRYLPVQNGVFTVSELERLVDQRTRVVSVSAVQFFG
                     GYRAPLAEIGAFCRSRGIIFCVDAIQWLGAGCLNVVTDHIDFLACGGHKWLMAGEGIG
                     FVYCRSDLAERLHPGEVGWQGVKQWLDFSDLRLDFQPGARRFTTGTVSAVGLHMLDAA
                     WSFLETIGWSAITAQVIALARRTAALAQERGWQPVIPPDHVASGIVSIRIPSDNLLPL
                     ITTLREQGIQISAREGALRVSPQFYNTDDDLDRLFQALDRLVPVSR"
     gene            3874..4590
                     /locus_tag="PKO06_04760"
     CDS             3874..4590
                     /locus_tag="PKO06_04760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV68988.1"
                     /translation="MFSAVQELAIGTGNLPVLLGFGYGESEEHRVSPRIITFHHPTHD
                     GHPVDQSGAPHGRKATPAGSDADLVRETLAGQNGAFDLLVDRHLSAAVGFFRYLRVPP
                     NRLEDLVQETFLRAFRKLELYDATRSFSVWLLTIGRNLYFDERRRCSFSEVPIEHIER
                     STPSPEDEIIERHTARELLDSLPDESRLLLELRIYRDLSFPEIAELTGEAETTLRVRF
                     HRLMLRLRLTVASGGEHENR"
     gene            4577..>5098
                     /locus_tag="PKO06_04765"
     CDS             4577..>5098
                     /locus_tag="PKO06_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68989.1"
                     /translation="MKTDNGCLQHLDALTEKSPLTPEALAHVKVCPACRQLAETLAAL
                     RQETTAWPTHDLSGLRQRVVARVRLETLPQSAAEPSRGERQPGPPPSVSWLDLLFAGR
                     TVGYAAVFVCLMVVSALWLHPWQSKPLQTMSAQEFRLVRANGVTETRLVNEKIELASE
                     TADLHLPDGSVLAV"
BASE COUNT         1037 a         1421 c         1608 g         1032 t
ORIGIN      
        1 ggacgcaaca acaagttccc tatgagtggg cggaactcac cgatatcacc tggttcgatg
       61 ctcgtatggc cacgattgtc ggtcttggca tggcgctgcg cacggatgat ggcggcgcta
      121 cgtggaaaca gttcgatcag gggttcaaac cacgcgccac cggaatcgtg cgggtgagcg
      181 acaccgtcgg atttctgctc ggccttgatg gaaacgtgat gaagacgacg aatgcgggca
      241 tgagctggcg caaggtctct gttcctggcg atgggcaccc ctatggcggg tggttcgtcg
      301 atgataagac cggttttctg agcggccaga ctggaattcg cgtgacgtat gatgggggcg
      361 agagctggag tgagttggcg gggagccctg atgatacgcg ggtgatcatc ttcacgagcc
      421 cgaccacggg ctatgcgttt ggtcgacgtt acagcggcgg gtgttttgga acgcatgctc
      481 cgcatgtatc ctgcactcgc gacggtggca agacctggac cgaacagcag accctgcgag
      541 atctgggcgg cctctgtgaa cgcctgattc agggcgaacc cggcatctgg atcggcgtcg
      601 ccggcggcac ggttttcaag ctcaagtttc tgtgaattcg caggtcagga cgcctctctt
      661 cccgagtgtg tgcccgaaag cccactggag cctgtgagag atcgcatttg tcctgcggct
      721 ctggcaattc ggtgtcggtt ctgatactgt gctgggcaac actttaatca tcatcttcgg
      781 agattcagca tgctcgcatc acgctctcat ctggtgacct atcgcgaaga cgcggccgac
      841 gccgagattc ccagccatcg cctgctgctg cgcgggggct atatcgtcaa agtcggggcc
      901 ggactctacg tctactcgcc gatcatgtgg cgcgtgctac ggaagatcga acgtatcgtg
      961 cgcgaagaac tcgatcgggc cggcgcgctc gagattcagg ctccgattct gcaaccggtc
     1021 gagtactggc aggaaagcgg ccgcctcgcg gtctataagg cctctggcac catgctgttc
     1081 accaaagatc gcaacggcgg tgaattcggg ttggccccga ccgccgaaga gatcgtcact
     1141 gccttcgtca aagccactgt tcagtcgtat cgacaggtgc ctctgactct gtatcagatt
     1201 cataccaaga tgcgtgacga gattcggccg cgcttcggtc tgctgcgtgt caaagagttc
     1261 atcatgaagg atgcctactc gttcgacatc gacaaggcag gcatggaaaa gtcgtatcag
     1321 gccatgaagg aagcctactt ccgtatcttt tcgcgcatag gcctcgaggc tctgggcgtc
     1381 gaagctgacc ccggcgatat cggcggctcg ggctcgatgg aattcatgat cgcggccaat
     1441 acgggcgagg acacgattct catcgaggaa ggaacgggct atgccgctaa cgtcgagaaa
     1501 gccgtgtcgc gactcgaacc gccggtcagc gcgaacgacg cactgaaacc gctcgagatt
     1561 cgcgatacgc cgaacattcg tacctgtgag cagttgcgtg atttcttcga tggcttcgcg
     1621 gtcgaacgaa tggtgaaaac cattctgtac aagttgacct acgccgaaga agagaaatac
     1681 tgcgcggtgt tgatgcgtgg aaacaaggaa gtcaacgaag tgaaactgaa aaaccacttc
     1741 ggctgtctgg cggtcagaat gttggcgggc gaagagattc aagcgctcac gggcgctgag
     1801 atgggctttg ccggcccgat cgggctcaac ccgaagttcc atctgctcgc cgatgagagc
     1861 gtgcgcggca tgcgcaatgt catctgcggc gtgaatcaga atcacaaaca tgcgctgcat
     1921 gccaatatcg accgcgactt caccccgaag gaatacgtcg acgtgcgcat ggcggtcgtc
     1981 ggagacgccg gtccgatcga gggcaagccg atgatcgtga agaaaggcat cgaagtcggg
     2041 cacattttcc agctgggcac caagtattcc gagccgatgc aggccaagtt cctgagcaag
     2101 gacggtaagc aggcggtctt tcacatgggc tgttacggcg tcggtgtctc acgtctggcg
     2161 gccgcggcgg tcgagcaaac ggccaccgac cagggatttg tctggccggc cgcgattgcg
     2221 ccattccacg tggccattac ctgccttgcc ggcaaggatc aggcggcggt tgcggccacc
     2281 gatcaggcct accgtgatct tcagggccga gggttcgacg tgctgctcga cgatcgtgat
     2341 ctcacggtcg gggtgcgcct gaaagacctt gaattgatgg gcattcccgc tttggtgctg
     2401 atcggcaaga agtggaagga gtcgggagaa gccgagatca agattcgcgc gaccggcaag
     2461 acgctgtttg ccaagctcga gcagttgccc gacgtcttga agcaggaagg ctgtttcgcg
     2521 taagtacgag tcgaaacacg ctcgcagttg ctgttgaggc ttctgcgagc gtttcttttt
     2581 tttagaattt ttgtttgata gtggcgtgtt tgagaggcac ggatgtcggt cagcgcgaaa
     2641 cgggaaccag tcggtcgaga gcctggaaca ggcgatcgag atcgtcgtcg gtgttgtaaa
     2701 actgtggcga aacccgcaga gccccctcgc gcgccgaaat ctggatgcct tgttcgcgca
     2761 gggtcgtgat gagaggcagg aggttgtcgc tggggatgcg aatcgagacg atccccgacg
     2821 ctacgtggtc aggcggaatg accggttgcc agccgcgttc ctgagccaat gccgcggttc
     2881 gccgcgccag agcgatcacc tgtgcggtaa tcgctgacca gccgatggtt tcgagaaagc
     2941 tccaggcggc atcgagcatg tgcagcccga cggcagacac cgtgccggtc gtgaaccgtc
     3001 gcgctcccgg ttgaaagtcg agccgcaggt cggaaaaatc gagccactgt ttgacgccct
     3061 gccagccgac ttctccgggg tgtaaacgct cggcgagatc cgatcggcag tagacgaagc
     3121 cgatgccttc gccggccatc agccacttgt gccctccgca ggcgagaaag tcgatgtgat
     3181 cggtgacgac gttcaagcac cccgccccga gccactgaat cgcatcgacg caaaagatga
     3241 tgcctcgtga ccggcagaag gctccgatct cggccagtgg ggctcgatac ccaccgaaaa
     3301 actgcactgc gctcaccgaa accacgcgcg tacgctggtc gacgagacgt tctagttccg
     3361 atacggtgaa gacaccattc tgcacgggaa gataacgggt ctcgacaccg ttgcgcgcct
     3421 gattgagcca agggtacaca ttggccggaa actcaatggc ggggagaatc acattgtctc
     3481 cggggctcca gtcgagcccc gaggcgagaa aactgatgcc ttcagaaacg ttgcgggtga
     3541 acgtgacctc atcggccaga cagcggagtg acgcggcgag gcggctgcgc aggtgctcga
     3601 gcagttgtcc ggtgtgttcc cagtggtgtt caccctgttg tgaacagcgg gcgagatagg
     3661 tttgtgccgc ttcgatgacc ggtgtgggta acgggccgat cgcagcgtgg ttgagatacg
     3721 accaggtgcg ggttatggga aacagagtct ggcgttcggc ttcggaaaac atcgcagatg
     3781 acctcgaaaa atgacgctgg gcgattatac atccgatgat gtgtaacggc ataccttctc
     3841 gctgcgtatg aagagtgcca ggaggtaacc ggcgtgtttt cggcagtgca ggaattggcg
     3901 atcggaacag gaaacttgcc tgttctgctc ggctttgggt atggtgaaag cgaggaacac
     3961 cgcgtgagcc ccaggatcat cacctttcat catcccaccc atgacggtca ccctgtcgac
     4021 cagtcagggg ctccgcatgg gcgcaaggcc acgcctgccg gttccgatgc ggatctcgtg
     4081 cgcgaaacac tcgcggggca gaatggggcg ttcgatctgc tggtcgatcg gcatctgtcg
     4141 gccgcggtcg ggtttttccg gtatttacgg gtgccgccca atcgacttga ggacctggtt
     4201 caagaaacgt ttctgcgcgc gtttcgcaag ctcgaactgt atgatgcgac gcgttctttc
     4261 tcggtctggc tcctgacgat cgggcgaaat ctgtacttcg atgagcggcg gcgctgctcg
     4321 ttttccgagg ttcccattga acatatcgag cggtcaacgc cctctcccga agatgagatc
     4381 atcgaacggc acacggcccg cgaactgctc gacagtctgc ccgatgagtc gaggttgttg
     4441 ctcgaattgc gcatttatcg cgatctgtcg ttcccggaga tcgcggaact gacgggagag
     4501 gccgaaacca cgctacgggt gcggtttcac cggctgatgt tgcgactgcg cctgacggtc
     4561 gcgtctggag gcgagcatga aaaccgataa tggttgcctg cagcatctcg acgcgctcac
     4621 ggaaaagagc cctctgacgc ccgaagccct ggcgcacgtc aaggtctgcc cggcgtgccg
     4681 acagctcgcc gagacactgg ctgcactgcg acaggaaacg accgcctggc ctactcacga
     4741 tctcagcggt ctgcgtcagc gtgtcgtggc gcgcgttcga cttgaaaccc ttccgcaaag
     4801 cgctgcagag ccttctcgag gtgaacgaca accggggcca cccccgagcg tctcgtggct
     4861 cgatctcctc ttcgccggcc gaacggtcgg gtatgccgcc gtctttgtct gcctcatggt
     4921 ggtaagtgcg ctctggttgc atccctggca aagcaagccc ctgcagacca tgtcagccca
     4981 ggagtttcgt ctggtacggg cgaatggggt gacagaaaca cgtctggtga acgagaaaat
     5041 tgaattggcc tccgagacag ccgatctgca cctcccggac ggatcggtgc tggccgtt
//
LOCUS       DAORHD010000160         5095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17111_length_5095_cov_3.209524, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000160 DAORHD010000000
VERSION     DAORHD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17111_length_5095_cov_3.209524"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4246)
                     /locus_tag="PKO06_04770"
     CDS             complement(<1..4246)
                     /locus_tag="PKO06_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68990.1"
                     /translation="MNKRSFPLIGGVLFLFIVCLLQLSPRFVPMLLAQSETPSAPPAS
                     PNPISAEPDTPSSAAPGSDQPVQPKLPPPKDLREAVLRGAQEGARKTLTDALKQLKEQ
                     VGDKLSEYQKAIFDFEAKKKEMEERIQKGLETAEQALNMAKAVRDLLYATSGYRGEIA
                     REPLIWAAKRMLKAASPLQAVMFESEADLIEDFLTMPKDRDPVDWIDRASTKMQVRLD
                     EMVKNLNKPAEEAIVKNADKFFGMFGTSFEEVSKIYHEQAEQSIARTMIELAKQEAVQ
                     EKLKDMAVNGKDWLKKRLKEAGNLDKFDDKAGAQREKINKINAEITQLEAKIRNLTQK
                     KKAVIANRRIHMGYVDKGRDVHARLKEEERKATDLEEQRQKLFEAANEFPAAQNAWVE
                     ASEQVEEIEKQLGRMQTQSWAAASKQEIKQRENEQRKLQTSLNQAKSKQEKLRQKRDE
                     AAKAKRAADALGEQVRKARANVDKLKGKMGSALDSSQDLEGENQKMDELTQQINAAKD
                     ELKDAFERRTKAADAWAKVRQKKERAQDVADLYEKGRTMGSRVFGEIDLPPEKAAVLI
                     EKAEKGYEEALRTIEYCKQKKEQFEGLLQQAKQIAEVGKGFRKLSEVFASRMEDIKTI
                     RKLISGGGVSLADLENAEISVVNFMTGSFGGMPQRCAAAMETIRGLDMTKLDAIFRDK
                     IGSSLGFVDDLGSARLSQMKERLSTMGADFGKQLASFEKFNGFVKQLQKKFDKLEELW
                     GGEKLEKFIKKISINDAYDKKVDELLSAPGDYLEKTIGKKNLNKIGKAAKFIAKFISK
                     WDLEDPSLAWVSPGPGELAYYHDKPVIVSLIASDANSGINPNPDMWWFSIDGKRVTSD
                     ATLQRFKFQPKTFEEFYFLETRHREGVAQVIIPPPAQTSGRSELTLKGWVRDRAGHAS
                     NEVNQKFFLQYDPDPPEIKVTAPPSDQPAGKAGRSFLAKVNISDTISRIREKDVIFCI
                     SSAENPDKCWIYRGSDKSRVTFAAAEGSYSSAADASYQHAEVTFKTPEELKDGLYILT
                     VRATNGAGMKNGVDHTFFIGDSYKDLVITPNFSPAKISFTSSETFDLRFTATNFSFME
                     IEDIEIKPHPVLPAGAPLERIASAETIVIRHVSGKSAKDNQDSFTVGVRTSDAYERFR
                     VGAVKENKRYDVKYEYQYKTAGNPEWKKKPLNVPVYLQAEEATRPTFVQGNLYYQSAD
                     TTGKIVDELPVPRGTQLRISQIYKFPTFGGDMPKYVEQFIGYGQVRDDTGFFRVPVQL
                     VGQSELRIRLVADALAVDDSQIDDKGRTVRKVMAAVAFPPAKIPYRCITEAFKLEEMA
                     PEADIEGDVRGQTVYQVGRRTLPYHHYGSGGKERGKGITITWEGELSTGGFISPFGIR
                     MQEGMFAASAFNAQANLLNPANPDMNGVFGTSPGLIW"
     gene            complement(4243..>5095)
                     /locus_tag="PKO06_04775"
     CDS             complement(4243..>5095)
                     /locus_tag="PKO06_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68991.1"
                     /translation="DDSVKPFALETQADVFWSRGNATEAAELYQRALVVEPLLISSRF
                     KRVLALLELGQFAPAFSEYLAMADWAVLPQAHHAMARLRAQFPELWQSLGDLFALALY
                     DALQDSSLDSTNSSHMKTAAAVAYALFNFDRARQYAAQARRFGASEPELAQLEALSQY
                     RSAEMAQRPLKEAEIQNLLDLLTPLAEKETSPLIEYVCARLCEARQRMETAQIHWQRA
                     LAEKGADPVILFANAQIALKKDDPAKARLLLEQLFQREFETNTAAIARQRYLSLLSRL
                     SEKERER"
BASE COUNT          930 a         1457 c         1436 g         1272 t
ORIGIN      
        1 gccagatcaa ccctggtgag gtgccaaaga cgccgttcat gtcagggttg gcgggattca
       61 gcagatttgc ctgcgcgttg aacgctgacg cggcgaacat gccttcctgc atgcgaatgc
      121 cgaaggggct gatgaagccg ccggtcgaca gctctccttc ccacgtaatg gtgatacctt
      181 tgccgcgctc ctttccgcca ctcccgtaat ggtggtaggg cagggtgcgc cggccgacct
      241 gatacacggt ctgaccccgg acatcacctt cgatatccgc ttccggagcc atttcttcca
      301 gcttgaaggc ttcggtgatg catcgataag gaatcttcgc gggcggaaag gcgacggctg
      361 ccatgacctt gcgtacggtg cgccccttgt catcgatctg tgagtcatcg acggcaagcg
      421 cgtctgccac gagacgaatg cgcagttcgc tttgtccgac cagttgcacc ggaacgcgga
      481 aaaagccggt gtcgtcccga acctggccgt aaccgatgaa ctgttcgaca tacttcggca
      541 tgtcaccacc gaaggtcgga aacttgtaga tctgactgat gcggagctgt gtccctcgag
      601 gaacgggaag ttcatcgacg atttttccgg tggtgtcggc tgactgatag tacaagttgc
      661 cttgaacgaa tgtcggcctg gtggcctctt ctgcctgtag atacacggga acgttcaacg
      721 gcttcttctt ccattcgggg ttccctgccg tcttgtattg atactcgtat ttgacgtcat
      781 atcgcttgtt ttctttgacg gccccgaccc tgaaacgctc atacgcatcg ctggtgcgta
      841 cgccgaccgt gaacgaatcc tggttgtcct tggcagactt tcctgagaca tgcctgatca
      901 cgatcgtttc ggcgctcgcg atccgctcga ggggcgctcc ggcggggagc acggggtgag
      961 gcttgatctc gatatcctca atctccatga agctgaagtt ggtggcggta aaacgcagat
     1021 caaaggtctc actgctggta aacgagatct tggctggtga gaagttcggc gtgatcacca
     1081 ggtctttgta cgagtcgccg atgaagaagg tgtggtcgac accgttcttc atgcctgctc
     1141 cgttcgtggc gcgaacggtc aggatataca gaccgtcctt cagttcttcg ggggttttga
     1201 acgtgacttc ggcatgctga tacgaggcgt cggctgccga cgaatacgat ccttcagccg
     1261 cggcgaacgt gacgcgcgat ttgtcgctgc cgcgatagat ccaacatttg tccgggttct
     1321 cagcagacga gatgcagaag atgacgtctt tttcacggat acgactaatg gtgtccgaaa
     1381 tattgacctt ggcgagaaaa ctccgaccgg ccttgccggc gggctgatca ctcggtggcg
     1441 cggtgacctt gatctcgggc gggtcgggat catactgcag aaagaacttc tggttgacct
     1501 cgttcgaggc gtgcccggcg cgatcgcgca cccagccctt cagtgtcagt tccgagcgcc
     1561 cggaagtctg cgccggcgga ggaatgatga cctgggcgac gccttcgcgg tgacgggttt
     1621 cgagaaagta gaattcctca aaggtcttcg gctggaactt gaagcgctgc agggtggcgt
     1681 ctgaggtaac tcgcttgccg tcgatcgaaa accaccacat gtccgggttc gggttgatgc
     1741 ccgaattggc gtctgaggcg atgagactga cgatcaccgg tttgtcgtgg tagtaggcga
     1801 gttctcccgg gcccgggctg acccaggcga gcgacgggtc ttcgagatcc cacttgctga
     1861 tgaacttggc gatgaacttc gcggccttgc cgattttgtt cagattcttc ttgccgatgg
     1921 ttttctcgag atagtctccc ggagcactga gcagctcgtc gaccttcttg tcataggcat
     1981 cattgatgct gattttcttg atgaacttct cgagcttttc gccaccccac agctcctcga
     2041 gcttgtcgaa cttcttctgc agttgtttga cgaatccgtt gaacttttcg aaactggcga
     2101 gctgtttgcc gaaatcggcc cccatcgtcg agagccgctc cttcatttga ctgagccgtg
     2161 ctgagccgag gtcgtcgaca aacccgagcg agctgccgat cttgtcacga aagatggcgt
     2221 ccagtttggt catgtccaga ccgcgaatgg tttccatggc cgcggcgcac cgctgcggca
     2281 tgccaccgaa actgccggtc atgaagttga ccaccgaaat ctcggcattc tcgagatccg
     2341 cgagagagac cccgcccccc gagatcagtt tgcggatcgt cttgatgtct tccatgcgcg
     2401 aggcaaagac ttctgacagc tttctgaacc cttttccgac ttcagcaatc tgtttggcct
     2461 gctgcagcaa cccctcgaac tgctcctttt tctgcttgca gtactcgatg gtgcgcaggg
     2521 cttcctcata ccccttctcg gctttctcga tcagtacagc ggccttctcg ggcggcagat
     2581 cgatctcgcc gaacacgcga ctgcccattg tgcggccctt ttcatacaaa tccgcgacat
     2641 cctgggcccg ttccttcttc tgccggactt tggcccacgc gtcggccgct ttggtccgcc
     2701 gttcgaatgc atctttcagc tcatccttgg cggcgttgat ctgctgcgtg agttcatcca
     2761 tcttctggtt ctcaccttcg agatcctggg aagaatcgag cgctgagccc atcttcccct
     2821 tcagcttgtc gacattcgcg cgggccttgc gtacctgctc gcccaaagcg tcagcagctc
     2881 gcttggcttt tgctgcctcg tcacgtttct gccgaagttt ctcctgcttg ctcttcgcct
     2941 ggttgagaga cgtctgcagc ttacgctgtt cgttctctcg ttgtttgatc tcctgttttg
     3001 atgccgcagc ccaggattgt gtctgcatgc gtccgagttg tttctcgatt tcttcgactt
     3061 gttcagaagc ttcgacccag gcattctgtg cggccggaaa ctcgttagcg gcctcgaaca
     3121 gcttctgtcg ctgttcttcg agatcggtcg ctttccgctc ttcctccttg agtcgcgcat
     3181 gcacatcgcg tcccttgtcg acgtaaccca tgtgaatgcg tcgattggcg atgaccgcct
     3241 tctttttctg cgtcagattg cggatttttg cctcaagttg tgtgatctcc gcattgatct
     3301 tgttaatctt ctcgcgctga gctccggctt tgtcgtcaaa tttgtccagg ttcccggctt
     3361 cttttaaccg ctttttgagc cagtctttgc cgttgaccgc catatccttg agtttttcct
     3421 gaacggcttc ctgttttgcc agttcgatca tggtgcgggc gatcgactgt tcggcctgct
     3481 cgtgatagat cttcgagacc tcctcgaacg acgtgccgaa catcccaaag aacttgtcgg
     3541 cattcttgac aatggcttct tcagcgggtt tgttcagatt cttcaccatc tcgtcgaggc
     3601 gcacctgcat cttggtcgag gcgcgatcga tccagtcgac cgggtcccga tccttcggca
     3661 tagtgaggaa atcctcgatc aggtccgcct cggactcgaa catgacagct tgcagtggag
     3721 aggcggcttt gagcatcctc ttcgccgccc agatgagcgg ttcacgggcg atttcgccgc
     3781 gatagcccga cgtggcatac agcagatcgc gtacggcctt ggccatattc aacgcctgct
     3841 ctgccgtctc gaggcccttc tgaatgcgct cttccatctc ctttttcttg gcttcgaaat
     3901 cgaagatggc cttctgatat tcggaaagct tgtcgcccac ctgctctttc agttgcttca
     3961 gagcgtcggt cagggtcttg cgagcgccct cttgtgcgcc tctgagaacc gcttctctca
     4021 ggtcttttgg cggcggaagc ttgggctgta ctggttgatc tgagccggga gctgcggatg
     4081 atggggtgtc gggctctgcg gatatcggat tcggtgaagc cggtggggca gagggagtct
     4141 ctgattgtgc cagaagcatt ggcacgaaac gaggtgacag ctgcagcaaa caaacgatga
     4201 aaagaaacag cacaccgcca atgaggggaa agctacgttt gttcatcgtt cgcgctcctt
     4261 ttcagaaagc cgtgacagca gactgagata ccgctgccgg gcgatggcgg ccgtattggt
     4321 ttcgaattcc cgctgaaaga gctgttcgag cagcagccgg gcttttgccg ggtcgtcctt
     4381 cttcagggcg atctgggcat tggcaaacag gatgaccggg tcggccccct tttccgccag
     4441 ggcccgctgc cagtggatct gggccgtttc cattcgctgc cttgcctcgc agaggcgagc
     4501 acagacatac tcgatcagag gagatgtctc tttctcggcg agcggagtca gcagatcgag
     4561 aagattctga atctcagcct ctttcagcgg gcgctgggcc atttcggccg agcgatactg
     4621 tgacagagcc tcgagctgtg cgagctctgg ttcgctggct ccaaaccttc gagcttgagc
     4681 cgcatactga cgcgcgcgat caaaattgaa tagagcgtat gccacagccg cggctgtttt
     4741 catatgcgat gaatttgtac tatcaaggga actgtcctgc aaagcgtcgt agagagcgag
     4801 ggcgaacagg tctcccagcg attgccacaa ttcgggaaac tgcgcgcgca ggcgggccat
     4861 ggcatgatgt gcctgtggca gaaccgccca gtccgccatt gccaaatact cagaaaatgc
     4921 cggcgcgaac tgcccgagtt caagcagagc aagaacacgc ttgaatcgag acgagatcaa
     4981 cagtggttcg accaccagcg ctcgctgata caactcggct gcttcggtgg cgtttccccg
     5041 gctccagaac acatccgctt gagtttcgag cgcaaagggc ttcaccgaat cgtcg
//
LOCUS       DAORHD010000161         5084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17160_length_5084_cov_4.584212, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000161 DAORHD010000000
VERSION     DAORHD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17160_length_5084_cov_4.584212"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..478
                     /locus_tag="PKO06_04780"
     CDS             89..478
                     /locus_tag="PKO06_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68992.1"
                     /translation="MKRLAGLLVVVLCLSASTVCAARSSKEQKMRQKAHEIGLMGQME
                     QANELMGGKGDLSKEGDTWNTSSLMLGLIWGAIGSGYFIYGKKQGRAVFLLCGVVLCV
                     FPMVITDVTTGTVVGLLLTFAPFKIDL"
     gene            complement(614..1729)
                     /gene="prfB"
                     /locus_tag="PKO06_04785"
     CDS             complement(614..1729)
                     /gene="prfB"
                     /locus_tag="PKO06_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_163545968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV68993.1"
                     /translation="MPNDFAPCDPSSPVSGGIFDLPKLQAKIADLEQLTLDPNFWNDR
                     NRAQEISQNIADMRKSCDLFSGVEKDFNDVAELIELSDADGDTAMVSELVGQLDPIQG
                     RLDQLELKTFLAEEFDESDCFLNIKPGAGGTESCDWASMLLRMYSRFLEKSDFTCEVL
                     DVQEEEQGIKSASLAIKGKYAYGYMKGEKGVHRLVRISPFDANARRHTSFAAVDVMPA
                     MPENVDIEVRDEDLDFDFYRSGGKGGQNVNKVSTAVRITHRPSGIVVSCQIERSQHQN
                     RERAMNMLKAKLYQLQKEESDRRLKGIQGDQKAISFGSQIRSYVFCPYTLVKDLRTGE
                     STGDVQRVMDGDINRFINAYLKWHAAGEKPRNVQEDE"
     gene            complement(1775..2233)
                     /locus_tag="PKO06_04790"
     CDS             complement(1775..2233)
                     /locus_tag="PKO06_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV68994.1"
                     /translation="MIRLTRRGIALPIVFGVILCLAVWIGSLSWTMSQSRFRFTQSLK
                     YRRAYFMARSALQHFFLKVKVMQRRNPEVMNTLYQAKPEKWPALAQTFVEDITQPMES
                     PGAYESSYRVASFSIDVQDTTKGEMVIQILAEGDVSSARESIRRVYKVTR"
     gene            complement(2230..2718)
                     /locus_tag="PKO06_04795"
     CDS             complement(2230..2718)
                     /locus_tag="PKO06_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV68995.1"
                     /translation="MKQRVATEPRPRRGITLLEVLVGLTLLTTVLLPIGMFLIEYLRG
                     STELGNSHQVISLVEEKMETALAQPFEALPIGVTESRRLVHDGRETIDLRPMELGPET
                     VKFTMTVEALPIEFSAIADGTSGRIEKVKVDDGFKKITVRAVWGRKTEHYFDLTAFKA
                     NL"
     gene            complement(2752..>5084)
                     /gene="secA"
                     /locus_tag="PKO06_04800"
     CDS             complement(2752..>5084)
                     /gene="secA"
                     /locus_tag="PKO06_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV68996.1"
                     /translation="RNVQEFTPIVNEGGILVPVGRGATLVTVNDYLARRDSEWMGRVY
                     KFLGLTVGLNVHGLDATEKRAAYNADITYGTNNEFGFDYLRDNMALRLADCVQRPEYN
                     FAIVDEVDSILIDEARTPLIISGPAEKATSLYYTFAGVVRDHLKSGEDYIIDEKHHGV
                     SVTEAGVAKVERVLGVQNMYDQKNMELVHHLQNALKAKHFFHLDKEYVVQDRKEGGKE
                     VVIVDEFTGRLMFGRRYSDGLHQAIEAKEGVKIQQENQTLASITFQNYFRLYRKLAGM
                     TGTAETEAREFKEIYKCEVVVIPPNRPLIRQDQSDIILKTAEEKFEAIVEHILEVHER
                     KQPILVGTISIEKSELIAAMLRQRGVTCQVLNAKYHEKEAEIVAQAGREGAVTIATNM
                     AGRGTDIQLGGNPEMLAWQETGQRDGPEFEAALQRFREACAAEKERVLASGGLFILGT
                     ERHEARRIDNQLRGRAGRQGDPGASQFYLSLEDDLMRLFGSANIAGWMEKLGWERGEP
                     IEHPWITKSIENAQRKVESHHFDIRKHVLKYDDVMNEQRTTVYKERRKALEKDDIHDD
                     MFSMAEDVVTDIMGRTVSPDIEPEDWDYNGLVENLQKVFPFQLSADELAKLSRDELYE
                     ELMKQVQAKFDDKKREVGEADFRQLEKFLLLQIVDGKWKDHLHNMDNLQDGIHLRSWG
                     QRDPLVEYKIEGFQMFNDMIESIKQDVLFYLFRMQYSAAEEREAIEEHEQEMYLNRGD
                     ETAQNAPKRAKEQIGRNDPCPCGSGKKYKKCCGQHP"
BASE COUNT          966 a         1415 c         1498 g         1205 t
ORIGIN      
        1 attcgctttt cccggcaaac aggcgtatgg tacgtggtaa ctctgaattt gtcagagagg
       61 tgtggtgccg tgacaaggag caggatgtat gaagaggctg gctggtctgc tggtggtggt
      121 gctgtgtctg agtgcgagta cggtttgcgc ggcgcgatcg tcgaaagagc agaagatgcg
      181 tcagaaagct cacgaaatcg gcttgatggg gcagatggaa caagccaacg aactcatggg
      241 cggcaaaggg gatctttcga aagagggaga cacctggaac acctcgtctc ttatgcttgg
      301 tctcatctgg ggagcaatcg gctctggcta cttcatctat ggtaagaagc agggaagagc
      361 cgtctttctg ctatgcgggg tcgttctctg cgtattcccc atggtcatta cagatgtgac
      421 gactggtacg gtcgtcggcc tgctgctcac gttcgctccc ttcaagatcg atctctgaac
      481 ggatcgaagg cacagccctg gactgtgcag gtacgagacc ttgatcgcca gaagaacctg
      541 caaaacaaca ggcgaaagat cttgagagat ctttcgcctg gagatgaacc tcaatctgac
      601 gaaacagcgc gggttattcg tcttcctgga cgttgcgggg cttttcgccg gcggcgtgcc
      661 atttcaggta ggcgttgatg aagcggttga tatcgccgtc catcacgcgt tgcacgtcgc
      721 ccgtcgactc gccggtgcgc agatctttca ccagcgtgta ggggcagaag acatacgagc
      781 gaatctgact gccgaagctg atggctttct ggtcaccctg aatacccttc agtcgccgat
      841 cgctttcttc cttctgtaac tggtacagtt ttgctttcag catgttcatg gcgcgctcgc
      901 ggttctggtg ctgactgcgt tcgatctggc acgacacgac aatgccggat gggcgatgtg
      961 tgatgcgcac ggcggtgctg accttgttga cgttctgccc gcctttgcca ccgctgcgat
     1021 aaaagtcgaa gtcgagatct tcgtcgcgca cttcgatgtc gacgttttcg ggcatcgcgg
     1081 gcatgacatc gacggccgcg aagctggtgt gacgacgggc gttggcgtcg aacggcgaga
     1141 tgcgtaccag tcggtgcacg cctttctcgc ccttcatgta accgtaggca tacttgcctt
     1201 tgatggccag cgaagccgat ttgatgccct gctcttcctc ctgcacgtcg agaacctcgc
     1261 aggtgaagtc ggacttttcg agaaaccggg aatacatgcg cagcagcatc gacgcccaat
     1321 cgcacgactc ggtgccgccc gcgccgggct tgatgttgag aaagcagtct gactcgtcga
     1381 actcttcggc cagaaaggtc ttcagttcga gctggtcgag tcggccctga atcgggtcga
     1441 gctgaccgac caactcggac accatggcgg tgtcaccgtc ggcgtcagaa agttcgatca
     1501 gttcggcgac gtcgttgaaa tccttctcga cgccggaaaa cagatcgcag ctcttgcgca
     1561 tgtcggcgat gttctgcgag atctcctgcg cgcggttacg gtcgttccag aagttcgggt
     1621 cgagggtcag ttgttcgaga tcggcgatct tggcctgcag tttgggcagg tcaaagatgc
     1681 cccccgatac tggagagctg ggatcgcatg gtgcgaagtc gttcggcaat ggcgaaatcc
     1741 atggctttac ctccttgaga tggtcgcgcg cgcgtcagcg cgtgactttg taaactcggc
     1801 gaatactttc ccgggcgctg ctgacgtcgc cctcggcgag aatctgaatc accatctcgc
     1861 cctttgtcgt atcttgaaca tcaatgctga agctggcgac gcgatacgat gactcgtacg
     1921 ctccgggcga ttccatcggc tgagtgatat cttcgacgaa ggtctgagcc agcgccggcc
     1981 acttctcggg cttggcctga tacagggtat tcatgacttc cgggttgcgg cgctgcatca
     2041 ctttcacttt gagaaagaag tgctgcagtg ctgaccgggc catgaaatag gcgcggcgat
     2101 acttgagtga ctgggtgaag cgaaaccggc tttgcgacat ggtccaggag agggagccga
     2161 tccagaccgc gagacagaga atgacgccga acacgattgg cagggcgatt ccgcgacggg
     2221 tgaggcgaat catagatttg ccttgaatgc cgtcagatcg aagtagtgct cagtctttcg
     2281 accccagacg gctcgtacgg taatcttctt gaagccatcg tcgaccttga ccttctcgat
     2341 gcgccctgac gtgccgtcgg caatcgccga aaattcgatc ggcagcgctt cgaccgtcat
     2401 ggtgaacttg acggtttcgg ggccgagttc catcgggcgc agatcgatgg tttcgcgccc
     2461 gtcatgaacc agtcggcgac tctcggtgac gccgatcggc agggcctcga atggctgagc
     2521 cagcgcggtt tccatctttt cttcgaccag actgatcacc tggtgcgaat tgcccagttc
     2581 ggtgctgcct cgcagatact cgatgagaaa catgccgatg ggtaacaaca ctgtcgtcag
     2641 cagcgtcaag ccgaccagca cctcgagcag ggtaataccc cgtcgtggtc tgggttcggt
     2701 tgctactctc tgtttcacga acatatcaat cgtggttcgc ctgactgtgc ttcacggatg
     2761 ttgcccacag cacttcttgt acttcttgcc actgccgcag gggcacggat cattgcggcc
     2821 gatctgttct ttcgcgcgtt tgggtgcatt ctgagcggtc tcgtcgccgc ggttcagata
     2881 catttcctgt tcgtgctctt caatggcttc acgctcttcg gcggccgaat actgcatgcg
     2941 gaagaggtag aagaggacgt cctgtttgat gctctcgatc atatcgttga acatctggaa
     3001 gccctcgatc ttgtattcga ccagcgggtc acgctgaccc caggagcgca ggtgaatacc
     3061 gtcctgcaga ttgtccatgt tgtgcagatg atctttccac ttgccatcaa cgatttgcaa
     3121 cagcaggaac ttttcgagtt gacggaaatc ggcttcgccg acttcacgtt tcttgtcatc
     3181 gaacttcgcc tgaacctgct tcatcagctc ttcgtacaac tcgtcgcgtg acaacttggc
     3241 gagctcgtca gcgctgagct ggaacggaaa cactttctgg aggttctcga cgaggccgtt
     3301 ataatcccag tcctcgggtt cgatgtccgg gctgaccgtg cgtcccatga tgtcggtcac
     3361 cacgtcttcg gccatcgaga acatgtcgtc atgaatgtcg tctttctcga gcgccttgcg
     3421 ccgctccttg tagacggtcg tgcgctgttc gttcatgacg tcgtcatatt tgagtacgtg
     3481 cttgcgaatg tcgaaatggt gagactcgac cttgcgctgc gcgttctcaa tcgacttggt
     3541 gatccagggg tgttcgatcg gttctccccg ttcccagccg agtttctcca tccagccggc
     3601 gatgttggcc gatccgaaca gacgcatgag atcgtcttcg agcgaaagat aaaactgact
     3661 ggcacccgga tcaccctggc gcccggcgcg accgcgcaac tgattgtcga tgcggcgggc
     3721 ttcgtgccgc tcggtgccga gaatgaacag gccgccggag gcgagtaccc gttccttttc
     3781 ggcggcacag gcctcacgaa agcgttgcag cgcagcttca aactcggggc cgtcgcgctg
     3841 accggtttcc tgccaggcca gcatctcggg gttgccgccg agctgaatgt cggtgccgcg
     3901 accggccatg ttggtggcga tcgtcaccgc gccttcccgg ccggcctgag ccacgatctc
     3961 ggcttccttc tcgtgatact tggcgttgag cacctggcag gtgacgccgc gctgtcgcag
     4021 catggcggcg atgagttcac tcttctcgat cgagatggta ccaaccagaa tcggttgttt
     4081 tctctcatgc acttcgagaa tgtgctcgac gatggcttcg aacttttctt cagccgtctt
     4141 caagatgata tctgattgat cctggcggat cagcgggcgg ttgggcggaa tgaccaccac
     4201 ttcgcacttg tagatttcct tgaattcgcg ggcttcggtc tcagccgtac cggtcatacc
     4261 ggcgagcttg cgatacaggc ggaagtagtt ctggaacgtg atcgacgcga gcgtctggtt
     4321 ttcctgctgg atcttgacgc cttctttggc ttcaatggcc tggtgcaggc cgtcagagta
     4381 ccggcgaccg aacatcagac gcccggtgaa ctcatcgacg atgacgactt ccttgccgcc
     4441 ttccttgcgg tcctgtacca catattcctt gtcgaggtga aagaagtgct tggctttgag
     4501 cgcgttctgc agatggtgaa cgagctccat gttcttctga tcgtacatgt tttgtacgcc
     4561 caaaacccgc tcgaccttgg cgacaccggc ttcggtaacc gaaacaccgt ggtgcttttc
     4621 gtcgatgatg taatcttcac ccgacttgag gtgatcgcgg acgacgccgg caaacgtgta
     4681 atacagcgag gtggctttct cggcggggcc cgagatgatg agcggtgtgc gggcttcgtc
     4741 gatgagaatc gagtcgacct cgtcgacaat ggcgaaattg tactcggggc gctgcacaca
     4801 atcagccagg cgcagggcca tgttatcgcg caggtagtcg aacccgaact cgttgttggt
     4861 gccgtaggtg atatcggcat tatacgcagc gcgcttttcg gtggcgtcga ggccatgcac
     4921 attcagaccc acggtgaggc cgaggaactt gtacactcga cccatccatt cgctgtcgcg
     4981 gcgggccaga tagtcgttca ccgtgaccag cgtcgccccg cgaccaaccg gtaccagaat
     5041 gcctccctcg ttgacgatcg gtgtgaattc ctgcacgttg cggg
//
LOCUS       DAORHD010000162         5053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17319_length_5053_cov_5.389556, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000162 DAORHD010000000
VERSION     DAORHD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17319_length_5053_cov_5.389556"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKO06_04805"
     CDS             <1..632
                     /locus_tag="PKO06_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HNV68997.1"
                     /translation="ITGTGRLAIMADVKAPTLLNISHNDRQELDDPNPVFEGALTDYG
                     CGLKPDTFKFLIDGVAQTGATLKADGTFRYEVRKTLVKGKHQIEFSVEDLAGNVLAQT
                     FEVVAPGAFAIGQIISYPNPVQGNNVWFSYNLEQAADDIKLKIYDAAGDKVAEFDTFD
                     FAVLKTGRVRWDLTNENGRRISNGVYFYKFEATKAGRTLKARGKLAVMR"
     gene            659..1303
                     /locus_tag="PKO06_04810"
     CDS             659..1303
                     /locus_tag="PKO06_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV68998.1"
                     /translation="MLNKKALILFLAALPLTIILSGCSGGVNGEGTNQYSQTNGTTVV
                     RGSSFNDSGWDLITKGQYESAITMFNKVLGDSPTDEEAAEANNGLGWAKSRLGKLEDG
                     MLWFERAISHSHDAKVGLAGAYIQRSSKADLEMAADLLYKQLGGENPHFKYTPRRQTG
                     VTDAECHAMLAYAFAAIGRAEEATEQLEYAKELQPEWASSTSDQIGRMVDFLMK"
     gene            1334..3130
                     /locus_tag="PKO06_04815"
     CDS             1334..3130
                     /locus_tag="PKO06_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV68999.1"
                     /translation="MMKRVLGVIVLLVLTLAVTGCLKTDSKVTGNIMGKVFDSNGHVL
                     RGASVEIYGGDHTVKTDELGRYYINGVEPGQKKVVATYEKKSVVKIFEIPRGGTLEGA
                     DLTFEVVDGLPPVITDVRVDALGENGATILWNTNELSNSLVDYALGPIGLGSYTWLAS
                     DSAMVEAHSVTLAGLTPNTTYHFRVRSFDYAGNEGISSDYQFHTPSGEAPSMPASFAV
                     SLPTEMERLTLTWAANSETDLLGYNLYRAESKAGPFVRVNADPIQASATSYIDEGLKI
                     AWKYYYYLKAVDTARNESAPTAVVAMLTPGMLIENRTWKESESPFVLYGDVRVRGGAV
                     LTVEPGVEVRCAMTDSLPDTNGASMTDIIVQGGLYAVGTSDKRIVFTSAETFPRLSSW
                     GGIRFLSTIEPENQLKYVTVMFADTGVRSEGSSPVIENTELGLCGSGLDIGLSAALNI
                     RYNTIRDCTVGLISASSNVRNNLFVDNQIAAALLGNDVFEHNTIDGFSGIEVTSGLPT
                     IRNNIISYIGTTKGLYGIKQTSALATPTISFNDIFNFTLETDGLTVATGSGNIASDPV
                     FVGGVPYNYQLQSTSPCLTAGDGGVQMGRYGP"
     gene            3536..>5053
                     /locus_tag="PKO06_04820"
     CDS             3536..>5053
                     /locus_tag="PKO06_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69000.1"
                     /translation="MHRFVGRQAIAGVTRVALFLIVALWCPCLVGAQIPNAKPASVST
                     LLQAQQLLQGGDRDQAFALLESCLTQEPGADEAARLYADALASAVVQLASGTSPDKLA
                     SASSAVCVAYLKRARDLQLDQPRYWIALLETLAALPKEQSSFTAESYRFFRRWSDRIE
                     NSSIPGVEKLLKEWERRFSEKTHPLEKWWLLRARGNSEPLKKEREALEGRIRKRLPEL
                     KQRFQEYLNRSEFAAADYTLDVLQQIDQTSTGLQELQTRLERVQKIQDLLTKASEAFK
                     AGKTDECRSLCQSVFALDPNNASAREMLKKLDESQRIPGQDLYGSSSRPSGAPAKELL
                     NRLSKAEQEEDFGEIKRVLKELTFQGMASAEHVSRLKEIDTEIENARMNLDLRWRDAQ
                     TLLDRQKWTELRRLLNRNPVFSSPERMMALEEMGLCCAHFLKSVDADKVQADAESLLK
                     RHPKSFWASVILMDQALGRNDFVTARKYYELAVAERTGHALLRWPRIILWLQMHGW"
BASE COUNT         1153 a         1334 c         1466 g         1100 t
ORIGIN      
        1 cgatcaccgg aaccggtcgc ctggccatca tggctgatgt gaaggcccca accctgctga
       61 atatcagcca taacgaccga caggaactgg acgatccgaa cccggtcttt gaaggggcac
      121 tcacggatta tggctgtggt ctcaaaccgg ataccttcaa gttcctgatc gatggtgttg
      181 ctcagacagg tgcgacgctg aaagccgacg gcaccttccg gtacgaagtt cgaaagactc
      241 tggtcaaggg caaacaccag atcgagttct cggtcgaaga tcttgccgga aacgtgcttg
      301 cgcagacgtt tgaagtcgtg gctccgggtg cctttgccat cgggcagatc atctcgtatc
      361 cgaacccggt tcagggcaat aatgtctggt tcagttacaa cctcgaacag gccgctgacg
      421 atatcaaatt gaagatctat gatgcggcag gtgacaaggt tgcggagttt gacacctttg
      481 atttcgcggt tctgaaaacc ggcagagtca gatgggatct caccaacgag aatggtcgac
      541 gcatctcgaa cggtgtctac ttctacaagt tcgaggcgac caaagccgga cgaacactca
      601 aagctcgcgg caaactcgcg gtgatgcggt aagtcccatg agcaaaggag gacagggtat
      661 gttgaacaag aaagcgttga ttctcttcct cgcggcgctg cctctgacga tcattctcag
      721 cggctgcagc ggcggggtga acggtgaggg aaccaaccag tactcgcaga cgaacggcac
      781 aaccgtcgtt cgtggcagca gcttcaacga ttcaggctgg gatctgatca ccaagggtca
      841 gtatgaaagc gcgatcacca tgttcaacaa ggtgcttggc gacagcccga ccgatgagga
      901 agccgctgag gcgaacaatg gtctgggatg ggcgaaatca cgcctgggca agctcgaaga
      961 cggtatgttg tggttcgagc gggccatcag ccactcgcat gatgccaagg tcgggttggc
     1021 gggggcttac attcagcggt cttccaaggc cgatctcgag atggcggccg atctcctgta
     1081 caaacagttg ggcggcgaaa acccgcactt caaatacacg ccgcggcgac agaccggagt
     1141 gaccgatgcc gagtgtcacg ccatgcttgc ctatgccttc gcggccatcg gtcgcgctga
     1201 agaggcaacg gagcagctcg agtatgcgaa ggaactgcag ccggaatggg cgagttctac
     1261 atcggatcag atcggccgca tggtcgattt tctcatgaag taatcgggat ctgaaaagga
     1321 agatcttttc accatgatga aacgggttct tggcgtcatt gttctgcttg tgttgaccct
     1381 tgccgtcacc ggctgtctga aaaccgactc gaaggtcacc ggcaacatca tgggaaaggt
     1441 atttgattcg aacggtcatg tgcttcgcgg agcatcggtc gagatctatg gtggtgatca
     1501 cacggtcaag accgatgagc tcggacgata ttacatcaat ggcgtggaac ccggccaaaa
     1561 gaaggtcgtg gcaacgtacg aaaagaagtc tgtcgtcaaa atcttcgaga ttccgcgcgg
     1621 tggaacgctg gaaggggcgg atctgacttt cgaggtcgtc gatgggttgc cgccggtgat
     1681 cacggatgtc cgggtcgatg cgcttggtga aaacggcgcg acgatcttgt ggaataccaa
     1741 cgaactgtca aactctctcg ttgattatgc gctcggtccg attggtctgg gttcctatac
     1801 ctggctcgca tctgactcag ctatggtcga agcgcactcc gtcactctgg ccggacttac
     1861 tccgaacaca acctaccact tccgggttcg ctcattcgat tatgccggca acgaaggcat
     1921 atcctcagac taccagttcc atactccctc gggagaagct ccgtcgatgc cggccagttt
     1981 tgccgtcagt ctgcctaccg agatggagcg cctgacactg acatgggcgg ccaactccga
     2041 aacggatctg ctggggtaca atctgtatcg agcggaatca aaggctggac cgtttgtgcg
     2101 agtaaatgct gatccgatcc aggcgtcagc cacttcctac attgatgaag ggttgaagat
     2161 tgcctggaag tattactatt acctcaaagc cgtggatacg gcccgcaatg agagcgctcc
     2221 gaccgcggtc gtggccatgc tgaccccggg aatgttgatc gagaaccgca cctggaagga
     2281 aagcgaaagc cccttcgttc tgtatggtga cgtgagagtt cgtggtggtg ccgtgctgac
     2341 tgttgaaccc ggagttgaag tgcgttgcgc catgaccgac agtctgccgg acacgaatgg
     2401 ggccagcatg actgacatca tcgtgcaggg tgggctctat gcggtcggca cctcagacaa
     2461 gcgcatcgtg ttcacctcgg ccgaaacatt tccgcgactg tcgagttggg gcggtatccg
     2521 cttcttgagc accatcgaac ctgagaatca gctgaagtat gtcaccgtca tgtttgctga
     2581 taccggagtg cgaagtgaag ggtccagtcc ggttattgag aacaccgaac tgggactttg
     2641 cgggagcggt ctggatatcg gattgtcggc ggctctcaac attcgctaca ataccattcg
     2701 cgactgcacg gtcgggctga tctctgcctc gtcgaacgtg cgcaacaatc tgttcgttga
     2761 caaccagatc gccgccgccc tgctcggaaa cgatgtcttc gaacacaaca cgattgatgg
     2821 attcagcggg atcgaggtga cgtccgggtt gccgacgatt cgcaacaata tcatttcgta
     2881 cattggcacg accaagggtc tttacggaat caagcagact tcggcgttag ccaccccgac
     2941 gatctcattc aatgatatct tcaacttcac tctggaaacg gatggtctga ccgtggcgac
     3001 gggcagtggc aacattgcgt ctgatccagt gtttgttggt ggagttcctt acaactacca
     3061 gttgcagtca acttctccct gtctgacggc cggcgatgga ggcgtgcaga tgggaagata
     3121 tgggccctga tcggcctcga aggtcgtgcg acaacatggt tgaagggcgg gtgaccgccc
     3181 ttcttgcatt tcagggaaac ccagggtcat ttaattctcc tccttactta ttttccgttc
     3241 acatcgctca acgattcacc gttacggcag ggagtgcgct cgtcgtctcg agaccgaggc
     3301 tgaaacctcg tcgacttcgc atccctgcga agacgacgag gccgaatcgc ctcctgcgat
     3361 ccggcagcct gttctcggac tccgcccgcc tcttgccgaa cggctcacaa gtttcgctct
     3421 tgcgaacgga aaataagcga gaattaaatg accctgaggg aaaccactcg ttcttgagga
     3481 atcaggccaa gtcagctatg ctgaagggga agatgtgagc aggagagaat cgcacatgca
     3541 tcgattcgtt ggccgacagg cgatcgccgg tgtgacgagg gtggcactgt tcctgattgt
     3601 cgccctctgg tgcccgtgtc tggtcggtgc gcagatcccg aacgcgaaac cggcatctgt
     3661 atccacactc ctgcaggctc agcagctctt gcaggggggg gaccgcgatc aggcttttgc
     3721 tttgctcgag tcttgtctta cccaggaacc cggggccgac gaagccgcgc ggttgtatgc
     3781 cgatgcgctt gcttcggccg ttgtccagct tgcgagtgga acaagccccg acaaactcgc
     3841 atcggcatcg agtgctgtgt gcgtggccta tttgaaacgt gcccgtgatc tgcaactcga
     3901 tcaaccacga tactggatcg cgcttcttga gactctggct gctctgccaa aagagcaaag
     3961 ttcctttacg gcagagtctt accgtttctt tcgtcgctgg agtgatcgca tcgagaattc
     4021 ctccattccc ggggtcgaga agctgttgaa ggaatgggag cgccgattct ccgagaagac
     4081 tcatccgctc gaaaaatggt ggctgctgcg ggcgcgtggc aacagcgaac ctctcaaaaa
     4141 agagcgtgag gctctcgaag ggcgcattcg caagcgatta cccgagctca aacagcggtt
     4201 tcaggaatac ctcaatcgaa gtgaatttgc cgcggcggat tacactctgg acgttctgca
     4261 gcagatcgat cagacgtcga caggccttca ggaattacag accaggctcg agcgggtgca
     4321 gaaaatccag gacctgttga ccaaagcgtc agaagctttc aaggccggca agacagacga
     4381 gtgtcgttcg ttgtgtcagt ccgtgtttgc actcgatccg aacaacgcca gtgcgcggga
     4441 gatgctcaaa aaacttgatg aatcacagcg cattcccggg caagatctgt acggaagttc
     4501 ctcaagacct tcgggagccc ccgcgaaaga actcttgaac cgcttgtcta aagctgagca
     4561 ggaagaggat tttggtgaaa tcaaacgggt actgaaggaa ctgacctttc aggggatggc
     4621 ttccgcagaa catgtgtcgc ggctgaaaga gatcgatact gagatcgaaa acgcgcgtat
     4681 gaacctggat ttgcgttggc gtgacgcaca aaccctgctt gatcgtcaga agtggactga
     4741 attgcgtcgt cttctgaata gaaaccctgt gttttcgtct ccagaacgaa tgatggcact
     4801 tgaggaaatg ggactctgct gtgcgcactt tctgaaatcg gttgacgcag acaaggtgca
     4861 ggctgatgcc gagtcgctgc tgaaacgtca tcccaagagc ttctgggcgt cagtcattct
     4921 gatggatcag gcactgggtc gcaatgactt tgtcacggcc cgaaaatact atgaactggc
     4981 cgtggccgaa cgaactggac acgccctgct gcgctggcca cgaatcatac tctggttgca
     5041 gatgcacggg tgg
//
LOCUS       DAORHD010000163         4999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17585_length_4999_cov_3.554207, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000163 DAORHD010000000
VERSION     DAORHD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17585_length_4999_cov_3.554207"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..1040
                     /locus_tag="PKO06_04825"
     CDS             87..1040
                     /locus_tag="PKO06_04825"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV69001.1"
                     /translation="MLEYTIRRLFGTVPVLLGILFIAFVVLYLIPGDPAKTLAGPRAS
                     EETIQRIAKDMGLDQPLHERFLRYLGRVLRGDLGMSMVNDRPVMTSILEKFPYTLRLA
                     LLALVISVVTGLLAGTLSALLQRTIWDRLITMLSVAGISVPIFLTGLILLYVFAVQLR
                     WFPTSAFGAKTMFMALFLPALTLGIRSGAFLARITRSTMIEVFNEDYIRTARAKGLSP
                     FRILWRHALFNALIPIVTVVALDLSSFLSGSVIVETIFELPGLGRFAMEAISKRDYPV
                     IQGVVLFSAVVFVGINLLVDLLYAYLNPRVRDEMLQARGNS"
     gene            1042..1872
                     /locus_tag="PKO06_04830"
     CDS             1042..1872
                     /locus_tag="PKO06_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV69002.1"
                     /translation="MRALWQRFTLIGRLSLLALLVLFLVALLAPMIAPHDPTETNLHL
                     RLAQSPGYPLGCDQDGQCLFSRLVYGTRLTLLIGFLARGLALLVGVGIGLAAGFWGGW
                     IDWLLMRVVDVFLAFPSLLLAIAISMVLGQGVATVILAIAVVGWAETARIVRGAALEI
                     RGAEFVMAARALGASDLRIMLTHILPNCLPLITVIFAMGMATAILSEASLSFLGLGVD
                     LSQPTWGSMVSLGRDYLIPHPRLSLYPGLCIGLVVILFNVLGDELRDLLDPGRKGQMT
                     "
     gene            1869..2663
                     /locus_tag="PKO06_04835"
     CDS             1869..2663
                     /locus_tag="PKO06_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNV69003.1"
                     /translation="MNKPIIGMAYSDCATRDHEMRVRTYCTHKYYWALQKAGAQVVLL
                     PPVSEDTDFERALSLVDGLLLPGGEDIDPVWQAEDPIPGLGLMNPWRDEYELRLAKTC
                     YERKIPVLGICRGIQIMAVALGGTLHQDIKGLPGVVKHEQQAPRWYPSHEVKLDAASV
                     LYQWLANVTSPESSCSRVNVKKSAPHRSSRTIRVNSFHHQMVKQVPKGCRAVAHSGDR
                     LIEALEATARDRVFVGVQWHPEELFDSDPCSRALFEGFVKAVQAAR"
     gene            2829..3320
                     /locus_tag="PKO06_04840"
     CDS             2829..3320
                     /locus_tag="PKO06_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69004.1"
                     /translation="MRRLTLSTLLGLMALWAVANAIGVDRTTASWSACLLSEQHVELA
                     VRNENTVPWDRFSLMARVYQGKTLAATTSFLQETKPLAAQAETRFKLPLTKKLTVGEA
                     YRVEVYLQRKGSPLVRKVFDERPATQISQATTTKKVAVIPEPRSMRDLIQPINVRKAM
                     GIP"
     gene            complement(3405..>4999)
                     /locus_tag="PKO06_04845"
     CDS             complement(3405..>4999)
                     /locus_tag="PKO06_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69005.1"
                     /translation="SAEPAAEPVAAKPAEPAKTEPDKPVRSAKRLPTNNLEYARDAVE
                     TFEKELPVLLDEFAKAKADNKLTTNDDDEGLVWLTLYKKIHRGFQNVPRYYFAAMKDG
                     WETSDPELAPLREKVLSLAKTAAQTGYWTCTNPDDALGSTDETQFEYWDRMRMKAFGA
                     MVWTNERKDPQQGQEIMAKVAKEADDMAATYAKDSQKPHPAFLRLTEEIARLQKECEA
                     GFKTAGNQKTEALAAWKALHDERERLQPFVGKIDMNQVHPNDYPTFIEEIEKFEKTDT
                     GIVKEKLEYLGKTYGNTPEALDASMQKLAGNEKPEGVVNEISYLVKRFDEIFQAVPRV
                     RKEVAESIANQATQELSGMESYDESIRAKKYEELLQSLVLGLRYDSHNTALIDLKAKV
                     EERAAKSAADVQKQIEERQWPGHHKGFSGPGNPDDLAKAAIEYFNSTCQSSEKALAAC
                     VVEPEWYCFKRNIFGQPVQWALTFWVAVDVEGQTTPDLVYAWSISFLTEENVGVEKKP
                     PFKLAAFNFKQKMKRAKVPGLK"
BASE COUNT          883 a         1376 c         1512 g         1228 t
ORIGIN      
        1 gcattcgtat gcgcccgtaa cgtcccgatg cctgacacac cggccccgga aaacgtttct
       61 ggcggccggt ttgccgttta accctgatgc tggaatacac gatcagacgc ttgttcggta
      121 cggtccccgt gttgctgggg atcctcttca tcgcgttcgt cgtgctgtat ctgattccgg
      181 gtgacccggc gaagactctg gccgggccgc gggccagcga agaaaccatt cagcgcatcg
      241 ccaaggacat gggtctcgat cagccgctac atgagcggtt tttgcgctat cttggccgcg
      301 tcctgcgcgg cgatctcggg atgtcgatgg tcaacgatcg cccggtcatg acctcgattc
      361 tggaaaagtt tccgtatacg ctgcgtctgg cactgctggc cttggtgatc tcggttgtca
      421 ccgggctgct ggccggaacc ttgagcgcgc tgctgcagcg tacgatctgg gaccgcctga
      481 tcaccatgct ttcggtggcg ggaatcagtg ttcccatctt tctgacgggg ttgattctct
      541 tgtacgtttt cgccgtgcag ctgcgctggt ttccgacttc ggcattcgga gcgaaaacca
      601 tgttcatggc gctgtttctg ccggcactga cactgggtat tcgctcgggt gcttttctgg
      661 cccgcatcac ccgcagcacg atgatcgaag tgttcaatga agattacatt cgcacggcac
      721 gcgcgaaggg gctttcgccg tttcgtattc tgtggcggca tgccctgttc aacgcgctca
      781 taccgattgt taccgtcgtg gcgcttgatc tgtcgagttt tttgagcggt tccgtcatcg
      841 tcgaaacgat tttcgagctt cccggtctgg gccgattcgc gatggaagcg atttccaaac
      901 gcgattaccc ggtcattcaa ggtgtcgttc ttttctcggc cgtggttttt gtcggcatca
      961 acctgctggt cgatctgctg tatgcgtact taaatccgcg ggttcgtgat gaaatgctgc
     1021 aggcgcgggg gaatagctga catgcgagcg ctctggcaga ggtttacgct gattggacgc
     1081 ctgagcctgc tggctctgct ggtgctgttc ctcgttgcct tgctggcccc gatgattgcg
     1141 ccgcatgatc cgactgaaac caatctgcac ctgcgtctgg cgcaatctcc cgggtatccg
     1201 ctcggctgcg accaggatgg ccagtgcctg ttttcccgcc tggtctacgg cacgcgatta
     1261 acgctgttga tcggctttct ggcgcgcgga cttgccctgc tcgtgggggt cggtatcggt
     1321 ctggctgccg ggttttgggg cggttggatc gattggctgt tgatgcgggt ggtcgacgtt
     1381 tttctggcgt ttccgagtct gctgctggcc attgcgatct cgatggttct cggccaggga
     1441 gtggcgaccg tgattctcgc cattgccgtg gttggatggg cggaaaccgc gcgcatcgtt
     1501 cgcggcgcag ctcttgagat tcgcggcgcg gagttcgtga tggcggcgcg tgcgcttggc
     1561 gcctctgatc tgcgcatcat gctgacgcat attcttccca attgtctgcc gctgatcacg
     1621 gtgatcttcg ccatgggcat ggccacggcg attctgagcg aagccagtct gagttttctc
     1681 gggcttggcg tcgatctctc gcagcccacc tggggttcga tggtctcgct tgggcgcgac
     1741 tatctgattc ctcacccgcg cctgtcgctg tacccgggcc tgtgtatcgg tctggtcgtg
     1801 attttgttca atgtgctcgg agacgagctg cgcgatctgc tcgatccggg ccgaaagggg
     1861 caaatgacgt gaacaagccg atcattggca tggcatactc ggactgcgcg actcgcgatc
     1921 acgaaatgcg ggttcgaacg tattgcacgc acaagtacta ctgggcgctg cagaaagccg
     1981 gagcccaggt ggtactgttg ccaccggtga gcgaggacac cgattttgaa cgggcactct
     2041 cgctggtcga cgggctgctc ctgccgggtg gtgaagatat tgatccggtc tggcaggccg
     2101 aagatccgat tccggggctg gggttgatga atccatggcg tgacgagtac gagttgcgcc
     2161 tggcgaaaac ctgctatgag agaaagattc ccgttctggg aatctgccga ggaattcaga
     2221 tcatggctgt ggctctgggc ggcacgctgc atcaggatat aaagggattg cctggagtgg
     2281 taaaacatga acagcaggct ccccgatggt atcccagcca tgaggtgaag ctcgatgcgg
     2341 cgtccgtgct gtaccagtgg ctggcgaatg tcacaagccc agagagctca tgctcgcgag
     2401 tcaacgtcaa aaagagtgcc cctcaccggt cgtcgcgcac gattcgagtg aactcatttc
     2461 atcatcagat ggtgaaacag gtgccgaaag ggtgtcgcgc cgtcgcgcat tcgggtgatc
     2521 gcctgattga ggcactcgaa gcaacggctc gagatcgagt ctttgtcggg gttcagtggc
     2581 acccggaaga actttttgac tctgatccct gttcccgggc tctttttgag gggttcgtga
     2641 aagccgtcca ggcggcacgc tgaagcgcct caccgccgat gattcgacca ccgggggccg
     2701 aaagccggga gaagctctcg gaaactcttt cgctgctgcc gccgcggtat cctggttgac
     2761 tcaacttttt ctgtcaaaat gccgatagat gtactgaaga agacaaaaat cacttcagga
     2821 ggcggcatat gcgaagactc accctgtcta ccctgctggg actgatggcg ttgtgggcgg
     2881 tggcgaatgc gatcggcgtc gaccgaacga ccgccagctg gagtgcctgc ctgttgtcgg
     2941 aacagcatgt ggaacttgca gtgcgcaacg agaacaccgt gccgtgggac aggttctcgt
     3001 taatggcacg ggtgtatcaa ggaaaaactc tggccgcgac gacatcgttc ctgcaggaaa
     3061 caaaaccact ggcggctcag gcggaaacgc gtttcaagct gccgctgaca aagaaactga
     3121 cggtgggcga ggcataccgg gtcgaagtgt atctgcagcg caaaggatcg cccctcgtgc
     3181 gtaaagtatt cgacgagcgc ccggccactc agatttcgca ggcaaccacc acgaaaaaag
     3241 tcgccgtgat tcccgagcct cgctcaatgc gtgatctgat tcagcccatc aatgtgcgca
     3301 aggccatggg cattccctga atacggatag aaaccacaac ggccaggcag aaatgcctgg
     3361 ccgttgtggc ttttcgtcac catgcccgag tgacgagaac ttgattactt cagaccggga
     3421 actttggcgc gtttcatctt ctgcttgaag ttgaaggccg ccagtttgaa cgggggtttc
     3481 ttctcgacgc cgacattttc ttcggtcagg aaggaaatgc tccaggcgta gacgagatca
     3541 ggagtggtct ggccctcaac gtcgacggcg acccagaagg tgagtgccca ctgaaccggc
     3601 tggccgaaga tgttgcgttt gaaacaatac cactccggct cgacgacgca ggcggcgaga
     3661 gctttttcgc tgctctggca ggttgaattg aaatactcga tcgcggcttt ggcgagatcg
     3721 tccgggttgc ccgggcccga gaagcccttg tgatgccccg gccactgtcg ttcttcgatc
     3781 tgcttctgga catctgccgc ggacttggct gctctttcct cgaccttggc tttcagatcg
     3841 atgagtgccg tgttatgcga gtcgtagcgc aagccgagga cgaggctctg cagtaactcc
     3901 tcgtatttct ttgcccgaat actctcgtca tacgattcca ttcccgacag ttcctgagtc
     3961 gcctggttgg cgatcgattc ggcaacctct ttgcgcactc gcgggacggc ctgaaagatt
     4021 tcatcgaatc gcttcaccag atagctgatc tcgttcacca cgccttcggg tttctcgttt
     4081 cccgccagtt tctgcatcga ggcgtcgagt gcttcgggag tgttgccata ggtttttccc
     4141 agatactcga gcttctcttt cacgatgccc gtgtcggtct tttcgaattt ttcgatctct
     4201 tcgataaaag tcgggtagtc gttgggatga acctggttca tgtcgatctt gccgacaaac
     4261 ggctgcaggc gttcacgctc gtcgtgaaga gccttccagg cggccagggc ttcggtcttt
     4321 tgattgcccg cagtcttgaa acccgcctcg cactctttct gcagacgggc gatttcctct
     4381 gtgagtctca ggaacgccgg atgcggtttc tgagagtctt tcgcgtaggt ggcagccatg
     4441 tcgtcagctt cttttgcgac tttggccatg atctcctgac cctgctgcgg gtccttgcgt
     4501 tcgtttgtcc agaccatggc accgaaggct ttcatgcgca tgcggtccca gtattcgaac
     4561 tgggtttcat cggtcgagcc gagcgcgtcg tccgggttgg tgcaggtcca gtagccggtc
     4621 tgagcggcag tcttcgcgag gctgagaacc ttttcgcgca agggagcgag ttcgggatca
     4681 ctggtttccc agccatcttt catggcggca aagtagtatc ggggaacgtt ctggaagccg
     4741 cggtgaatct tcttgtacag agtcagccag accagccctt catcgtcgtc attggtcgtc
     4801 agtttgttgt cggccttggc cttggcaaac tcgtcgagca gaaccggcaa ctctttttca
     4861 aaggtctcaa cggcatcgcg agcgtattcc agattgttgg tcggcagacg cttggcagag
     4921 cgtacgggtt tgtctggctc ggtcttcgct ggctcagcgg gttttgcggc tacgggttct
     4981 gctgctggtt cagccgagg
//
LOCUS       DAORHD010000164         4988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17642_length_4988_cov_3.250963, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000164 DAORHD010000000
VERSION     DAORHD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4988
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17642_length_4988_cov_3.250963"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PKO06_04850"
     CDS             <1..692
                     /locus_tag="PKO06_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV69006.1"
                     /translation="RGLVLPTLPAEFETQALTPVYLAGRGKLRGVFGLRDQPRPESVE
                     VVRELKSLGITLVMASGDREAPVRAMARETGIEEAHAGLVPEQKLALVRNYQTRGVVG
                     FVGDGLNDAPSLAAANVGLAIGSGADAAKENTDLVLAGHDLRVLPFAIRLARATLAKI
                     RQNLAWAVVYNLAALPLAAGILAPFLGPRWSLTPEVAGLAMAFSSVSVVANSLLLRAA
                     AFAAVVRRERA"
     gene            773..3676
                     /locus_tag="PKO06_04855"
     CDS             773..3676
                     /locus_tag="PKO06_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69007.1"
                     /translation="MTTEQVQASGLAFPKTLDLDEWLIFSLDKGDEGNRLLVLEELRV
                     RTLSDEFAARLQTVSAKDPAPACRRAAVEILAARQAAEKGRKLAVQAQLTPDMYQALA
                     EQADPAFREALLRSIRKAPSPEILDMWRAQLLGAPSPEVTEVALTLLARFGRPEDAGC
                     AAVYLASDNPEHVKAAIDLLHAHNIDSFKEQIALPLASSHMGIKMHAIRRLRSVDAAE
                     AMIYLRMLLSDSDPLIRQKALRELLLVPFHEAETAYLQFLSTETRPTLLVAAGLAVAF
                     NPDPELPLKIYDIFLVSRGAKQQILQLVLNELVDSIRQAGILKESPQEYVARLKERLQ
                     KRRQVLLIQHTIKELANPDASMRQAAVQRLRPYAGDPVVSEALRRRLPEEPDEDIKAI
                     LVACTAQAEQQAVQHLVVAVTSGDFYDLPLPQQRSLVNSVHDQAGYATARASLKVLLL
                     GNLHRSVLLETVVRIGSFGQAGDVTVLLPLLQHAEPGVVAAAVKACGQLDVDALLPTL
                     NKLLQHDDPRVKAAALEVFVLADKEGAVQYLSSMVRATSPTTRRQALTLMPLLDYPSA
                     EPILTYLFLSEPVEEIKLQAGYMLAANPTRDGIKTLYRGTHADQGKIKRGWQELWDAG
                     IATAEQTLGLKREEIEAQCVTQLDAEKKAQEAPAPKYAFQKVVGTKAGVGMPASLLGV
                     EPELTTEQKIEKLKSQLWEMRMQVLIALVILSPLAWFFVFGTSGTPGVKTDLLDRKPR
                     RTYSGSGPAAPMQQMTVQGLQSNVDQVLGGDSYAGFMQSVDDELEEIDRQIEKSRRER
                     IEKILEDRQATFWQRMAAAAALDERLQQAEQALTENRLSDAEDIFLKILADPEARPYV
                     KAWAYQGLMQLALSGRGTMADFARYSDGYFALLNADKPPAADGTKVQNVSEKLLKLDE
                     IGRQFQNQDFLKHLRSALQKGEKLSEPEADQRVTQMMEGMKSLPSMKDTGEHD"
     gene            3764..>4988
                     /locus_tag="PKO06_04860"
     CDS             3764..>4988
                     /locus_tag="PKO06_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69008.1"
                     /translation="MASQQPNRSVLSRSASAYLVAIGALSLMVIVILSFSQTTISNRW
                     TAIFSNHEKKAEECAEAACQLTFRTAQERINDRKQFYGALKNPTKLFQNWFMHFRIPT
                     FVAEANFDPVSWQGSHPNGMDVGLDLFNNGIYKPLYENGIQATYTYDVSNPANTQSSC
                     TDSGPNHLEAMSNMLESMGGKCTVHIRARIARAFGILARDPQYKVPGINVKLPSLGTD
                     GPVGKILDGIFGSPPPCFEFNALDYLHDKNLVKEFLKQALDDLEIKALTPYSPIPLWI
                     PLGKLLNMLGIGDVLSDGIATALGLENVSLKGIAKLVAQKLGLSDMLTLKIDFGEFTD
                     NIKKHIDNMFPSQLDFLKSSFGFDITVEKQGVLEVRATVTYQPYFPNPGPSIKKTLVM
                     EREFRCADVQPIAPDH"
BASE COUNT         1083 a         1355 c         1472 g         1078 t
ORIGIN      
        1 cgcgaggact tgtactgcca acactgccgg cagagttcga aacccaggcg ttgacccccg
       61 tctatttggc tgggcgcgga aagctgcgcg gtgtctttgg actgcgtgac cagccgcgtc
      121 cggaatcagt ggaagtggtg cgtgagctta aatcgctcgg catcaccctg gtcatggcca
      181 gcggcgatcg tgaggcgccg gttcgagcga tggctcgtga aacggggatc gaagaagcgc
      241 atgccgggct ggttcccgaa cagaagttgg ccctggtgcg gaactaccag acccgtggag
      301 tggtgggttt cgtcggggat ggactgaatg acgctccgtc gctggctgcc gcgaatgtcg
      361 ggctggcaat cggatcagga gcggatgcgg caaaggagaa caccgacctg gtacttgctg
      421 gtcatgatct gcgtgtgctg ccgtttgcca ttcgtctggc gcgtgccacc ctggcgaaaa
      481 tccgacaaaa ccttgcctgg gctgttgtgt acaacctggc cgcgttgccg ttggctgcgg
      541 gaatactcgc tccctttctg gggccgcgat ggtcgctgac ccctgaagtt gctggcctgg
      601 ccatggcttt ctcatcggtc tcggtggtcg cgaactcgtt gttgttgcgt gccgctgctt
      661 ttgctgccgt cgttcgccgt gagcgtgcat aggcggttcg tgcgctgcct ctcccctgag
      721 gaacgactgc gggaacgttg gaaaaccggt cttgccggtg tatactgtcg gtatgacgac
      781 tgagcaggta caggcttctg gtctggcctt tcccaaaacc cttgatctcg atgaatggct
      841 gatcttttct ctcgacaaag gagatgaggg aaatcgcctg ctggttctcg aagaactgcg
      901 ggtacgcacg ctctcggatg agttcgccgc tcgcctgcag accgtgtcgg ccaaagaccc
      961 tgctccggcg tgccggcgtg ccgccgtcga gattcttgcc gcgcgacagg ctgccgaaaa
     1021 agggcgcaag ctcgcggtgc aggcgcaact cacccccgat atgtatcaag cgctggcgga
     1081 gcaggccgat ccggcctttc gcgaagcact gctgcgctcg attcgcaaag ccccttcacc
     1141 tgaaattttg gatatgtggc gcgcgcaact tctgggcgca ccatcgcccg aagtcaccga
     1201 agtcgcgctg acgctgctgg caagattcgg tcgccccgaa gatgcaggat gcgcggcggt
     1261 gtatctggct tccgacaatc ccgaacatgt caaagctgcc attgatctgc tccacgcaca
     1321 taacatcgac tccttcaagg aacagattgc tcttccgctg gcttcttccc acatgggaat
     1381 caagatgcac gccattcgcc gtctgcgcag tgtcgatgcc gccgaggcaa tgatctatct
     1441 gcgcatgctg ttgagcgatt ccgacccatt gattcgtcag aaagccctgc gtgaactgct
     1501 cctggtgccg ttccatgaag ccgaaaccgc gtatctgcag tttctcagca ccgaaacccg
     1561 cccgactttg ctggtggcgg cggggcttgc cgttgccttc aatcccgacc ccgagcttcc
     1621 gctcaagata tacgacatct ttcttgtctc tcgtggagcc aaacagcaga tattgcagct
     1681 ggtactcaat gagttggtcg actcgatcag gcaggctgga atcctcaagg aatcaccaca
     1741 ggaatatgtt gcccgcttga aagagcgact gcagaagcgg cggcaagtac tgctgataca
     1801 gcatacgatc aaagaactgg cgaatcccga tgcgtcgatg cgtcaggccg ctgtacagcg
     1861 gttgcgtccg tatgcgggtg acccggttgt gtcagaggcg ttgcgacgac gtctgcccga
     1921 agaaccggat gaagacatca aagccattct ggtggcttgt acggcgcagg cggaacagca
     1981 ggcggttcag catctggtcg tggcggtcac ttcgggcgat ttctacgatc tgcctttgcc
     2041 gcagcaacgt tcgctggtga actcggtgca tgatcaggcc ggatatgcga cggcacgtgc
     2101 gtctctgaaa gtgttgctgc tcggtaatct gcatcggtcg gtgctgctcg aaacggtcgt
     2161 gcgcattggc agctttggac aggctggcga tgtgacagtg ctgctgccgc tgttgcagca
     2221 tgcggaaccc ggtgtggttg ccgctgccgt gaaagcgtgc gggcaactcg acgtcgatgc
     2281 gttgctgcca acactgaaca aactgttgca gcacgatgac ccgcgagtga aagctgccgc
     2341 gctcgaagtc ttcgtgctgg ccgataaaga gggggcagtc cagtatctct cttcgatggt
     2401 ccgggcaacc agtccgacca cgcgtcgaca ggcgttgaca ctgatgccgc tgctcgatta
     2461 tccgtcggct gaaccgattt tgacctacct cttcctttcc gaaccagtcg aagaaatcaa
     2521 gctgcaggct ggatacatgc tggctgccaa cccaacccgc gatggtatca agaccctgta
     2581 tcgtggaact cacgccgatc agggaaaaat caaacgtggc tggcaggaac tgtgggatgc
     2641 gggaattgcc acagccgagc agacattggg tctgaagcgc gaagagatag aggcgcagtg
     2701 cgtcacccag ctcgatgccg aaaagaaggc gcaggaagct ccggcaccga agtatgcttt
     2761 tcagaaagtt gtaggaacca aagccggagt gggaatgccg gcgagtctgc ttggggtcga
     2821 acctgagctg acgaccgaac agaagatcga aaagctgaag tcccagttgt gggaaatgcg
     2881 gatgcaggtt ctgatcgccc tcgtgatcct ttctccgctg gcctggtttt ttgtcttcgg
     2941 cacgtcgggc actccagggg tgaaaacgga tctgctcgat cgcaagcctc gacgcacata
     3001 ttccggttcc gggccagcgg ctccgatgca gcagatgacg gtacagggct tgcagagcaa
     3061 tgtcgaccag gtgcttggcg gcgattcata cgccggattc atgcagagtg tcgatgacga
     3121 actcgaagaa atcgatcgac agatcgaaaa gtcgcggcgc gaacgaatcg agaagattct
     3181 tgaagaccga caagccacgt tctggcaacg tatggcggcg gcagctgctc tcgatgagag
     3241 attgcagcag gcggagcagg ctttgaccga gaaccgactg agtgacgctg aagacatttt
     3301 tctcaagatt cttgctgacc cagaagcgcg cccgtatgtc aaagcgtggg catatcaggg
     3361 attgatgcaa ctggctctct caggcagagg aacgatggcg gatttcgccc gatattccga
     3421 tggctatttt gcgttgttga atgccgacaa accgccggca gcagatggta caaaggtgca
     3481 gaacgtcagc gaaaaactcc tgaaacttga tgagatcggt cggcagtttc agaatcagga
     3541 ttttctcaaa cacctgcgat ctgcattaca gaaaggggaa aaactgtcgg aacccgaggc
     3601 ggatcagcgg gtcacgcaga tgatggaggg gatgaaatct cttccctcga tgaaagatac
     3661 cggagagcat gattgatccc ggcagaacgg ttcgcgaacc ttttggttcc tgacagcgta
     3721 ttattcgtac gatatactga tcaatagagg ggcagaaaag cgtatggcta gccaacaacc
     3781 gaatcgatcc gttctttcgc gcagcgcttc ggcctatctg gttgccattg gagcgctcag
     3841 tctgatggtc atcgtcattc tgagtttttc gcagacgacg atcagtaacc gctggaccgc
     3901 catcttctcg aaccatgaaa agaaagcgga agaatgtgct gaagccgcct gccagttgac
     3961 gtttcgtacg gcccaggagc ggatcaacga tcgcaagcag ttctacggag cgctcaagaa
     4021 ccccacgaag ctcttccaga actggtttat gcacttccgc attccaacct ttgttgccga
     4081 ggccaatttt gatccggtca gctggcaggg atctcacccg aatggtatgg atgtcggcct
     4141 cgatctcttc aacaacggga tctacaaacc gctgtatgaa aacggcattc aggcgactta
     4201 cacgtatgat gtttccaacc cggcaaatac gcagagttcc tgcaccgaca gtggccccaa
     4261 tcatctcgaa gccatgtcga acatgcttga atccatgggg ggaaagtgca cggttcacat
     4321 ccgggcgcgt atcgcgcgtg cttttgggat tcttgcacgt gaccctcaat acaaggttcc
     4381 gggaatcaat gtcaagctgc cgagcctggg cacggacggc ccggtaggaa aaattctcga
     4441 tggcatcttt ggcagtccgc cgccgtgctt tgagttcaat gctctcgact atcttcacga
     4501 caagaacctg gtgaaagagt ttctcaaaca ggcactggat gatctggaaa tcaaggccct
     4561 gacgccctat tctccaattc cgctctggat accgctgggt aagttgttga acatgctggg
     4621 tattggtgac gtgctttcgg atgggattgc cacggcgctg gggcttgaga acgtgtcgct
     4681 gaaaggaatc gccaaactgg tggctcagaa actcgggttg agcgacatgc tgacgctcaa
     4741 gatcgatttt ggcgaattta cggacaacat caaaaagcac atcgacaaca tgttccccag
     4801 ccagctcgat tttctcaagt cgagtttcgg gttcgacatt acggtcgaaa agcagggcgt
     4861 gctcgaggtg cgagcgacgg tgacctacca gccgtatttt cccaacccgg gaccatcgat
     4921 caagaagacc ctggtcatgg agcgcgaatt ccgctgtgcg gatgtgcagc cgattgcccc
     4981 tgatcaca
//
LOCUS       DAORHD010000165         4974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17701_length_4974_cov_4.085790, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000165 DAORHD010000000
VERSION     DAORHD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17701_length_4974_cov_4.085790"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKO06_04865"
     CDS             <1..519
                     /locus_tag="PKO06_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69009.1"
                     /translation="VAPAPNTRVVLTFCATKAEKQEMDKRIMARYEGQKKDAISKKKL
                     TKRLGERVRQKVIKNKIEFMIDSLNEMDHATMTIDRGNTPEMVKSASLVAKFESPERA
                     AQIKKNIMKHLVKEIKRTDNVNDKFALGNVSVTTQGSELIMRCKFQDAKEQMHGFNLI
                     SSYVAKGMLDRL"
     gene            complement(648..1631)
                     /locus_tag="PKO06_04870"
     CDS             complement(648..1631)
                     /locus_tag="PKO06_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV69010.1"
                     /translation="MNTRARGVSARNLIDFHTHLTLHWNTLPALVRAHHPRRIDPLAP
                     LLRSTFWRSYEALRYQNLVRRRSLFEGPLERVLNRIIRSFHREEPEDLLTRMDRCGIA
                     ASVVLAVPPVVPNEAVLRACAQSHRLIPFISPLPDLPPEPQISRLLQAGGRGIKVHPL
                     LQHVIVDSDFVTRIAQEAARYQVPLVIHAGGSGRMFGLDAHHRTEPEEFARLARRVPE
                     AQIVIAHAGLWESPEIMVAAAPHDNLFLDVSFQSPAALRELRRRIPPHRLLLGSDSPM
                     GNIEIVIDNCVQAGFTREELDSLFWKNAKRLLGEPQIDERPAPSAGNLPRI"
     gene            complement(1664..2908)
                     /locus_tag="PKO06_04875"
     CDS             complement(1664..2908)
                     /locus_tag="PKO06_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69011.1"
                     /translation="MNTFFIVTSAHACTELIVETNQLRDYLVKNDFTEVGRREEADLI
                     LVSTCSFNQQYEDEAYRNITESRTNRKPGAQVIVSGCYPKIAPHVFKEFPDVVDIPPL
                     NMEKIETIIAPKRTSFRDSVTNSVNDHEYLKSSTFMVGRRLKNAVTRVQKYLPFVQMP
                     NWLDSLPMTDWYFIQGGNGCLGNCTFCAIKHARGKIKSTGLDQIMPQLERAVRRGYKT
                     ISFTGTDMGCWGQDIGSSLPDLLNEVVKVPGDFVVDMHYVEPDWLIKYIDRLEPVFQS
                     GKIRSFGSPVQSGSNRILKLMGRNYVIEDYIRVVNHVIDNTRVKSMNSIVMVGFPRET
                     KEDFLETYNLINKVKISYWCLLKYEGRHNIPSEKLDGKVPEEIAVNRLQRIERKAKLR
                     NYVGLSDALVEKIVRKCYGPVR"
     gene            complement(2947..3564)
                     /locus_tag="PKO06_04880"
     CDS             complement(2947..3564)
                     /locus_tag="PKO06_04880"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69012.1"
                     /translation="MQIHHPGEWTGPRGRFHAWFFGTWTRKMLELLCFGNYWTSFKRE
                     LIRRVRHGNERVLDVGAGSGNFSLPIARRLKGGTIYCLDSSIEMTDRLQAIAARQAVR
                     ERIEVLNCDAGQTGLPDQSIDWVVSGNCMHEMSEPAKIWAEVYRVLKPGGAAFIVDFR
                     DRHGYHDDAHGPYSVEQMQTLYTDAGFRTIHVEPQRHFVIGIAEK"
     gene            complement(3708..4271)
                     /locus_tag="PKO06_04885"
     CDS             complement(3708..4271)
                     /locus_tag="PKO06_04885"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV69013.1"
                     /translation="MVRKSTTAIQPRSRTFLFFCLLFILLIPCGFLFFGIREFVQVMR
                     VYRWHIVPVTVEGCHPTVEEHPPDEDQPETRYSLSFTVSYRYQVASHSYSGSWNTGQN
                     LGKVRPSISEVASLTTAFRQTLGDGFSVRVDPDNPERSLVEPSLVAPLILIGAGLFSG
                     AFFLTMLVPMWRNPEPESGGSADTTLD"
     gene            complement(4271..4909)
                     /locus_tag="PKO06_04890"
     CDS             complement(4271..4909)
                     /locus_tag="PKO06_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69014.1"
                     /translation="MPVSLGLALYFVLAVDHFSARSLTYSLIVSTVLFLTTYEFGANR
                     LQPRQTSLILAFVCALTGVVFLSRGLYLFVYPEVQMFTPTWQNAVLFFLAPLLEIGFV
                     VGFIMLNSQRLEQELVESQQQLKDNVASLERAIGEVKTLSGLLPICAHCRKIRDDQGY
                     WQRIELYISSRTEAEFTHCLCPPCAKELYPELQLEKTSARDPNPKPPQAPAA"
BASE COUNT         1119 a         1295 c         1527 g         1033 t
ORIGIN      
        1 gtggctccgg cccccaacac tcgtgtcgtt ctgaccttct gcgcgaccaa agccgaaaag
       61 caggaaatgg acaagcgcat catggctcgc tatgaaggcc agaagaaaga tgcgatttcg
      121 aagaagaaac tcacgaagcg cctgggtgaa cgtgttcgcc agaaggtgat caagaacaag
      181 atcgaattca tgatcgactc cctgaacgaa atggatcacg cgacgatgac catcgatcgt
      241 ggcaacaccc ccgaaatggt caagtcggct tcgctggtcg ccaagttcga gagccctgaa
      301 cgcgcggctc agatcaagaa gaacatcatg aagcacctgg tcaaagagat caagcgcacc
      361 gacaacgtga acgacaagtt cgcgcttggc aacgtcagcg tgacgacgca gggaagcgaa
      421 ctgatcatgc gctgcaagtt ccaggatgcg aaagagcaga tgcacggctt caatctgatc
      481 tccagctatg tcgccaaagg catgctcgat cgtctctgat tctcgtttcg ttctgccgca
      541 aacccgccga gagattctcc cggcgggttt gtttttgcgc atgacggcat ggtgtgaaga
      601 ctgccgcgcg tccgcacgcg actgtacaag cgtgtgaacc tgatacttta gattcggggc
      661 aggttacctg cggaaggtgc tgggcgttcg tcgatctggg gttcgccgag caatcgtttt
      721 gcgttcttcc agaatagcga atcaagctcc tctctcgtga aaccggcttg tacgcagttg
      781 tcaatgacga tctcgatgtt tcccatcggc gaatcgctgc cgagcagcag gcgatgcggc
      841 ggaattcggc ggcgaagttc acgtagtgcg gcaggactct gaaagctgac gtcgagaaac
      901 aggttgtcgt gcggggccgc ggcgaccatg atctccgggc tttcccagag ccccgcgtgg
      961 gcgatgacta tctgcgcttc gggaacgcgt ctggccagtc gggcgaattc ctcgggctcc
     1021 gtgcggtgat gggcatcgag accgaacatt ctgccgctgc ctccggcgtg aatcaccagg
     1081 ggaacctgat accgcgcggc ctcttgagca atgcgggtga cgaagtcgct gtcgacgatc
     1141 acgtgctgca gcagggggtg caccttgatc ccccgacctc cggcttgcaa caaacgagag
     1201 atttgcggtt cggggggcag gtcgggaaga ggagagatga acgggatcag tcggtgcgat
     1261 tgggcacaag cccgcaacac ggcttcgttc ggtacgaccg gtggcacggc gagaacgacg
     1321 gaagcggcga taccgcagcg atccatgcgg gtcagcaggt cttccggttc ctcgcggtga
     1381 aaactgcgaa tgatgcgatt caacacgcgt tcgagtgggc cttcgaacaa actgcgtcgt
     1441 cgtaccaggt tctgatagcg cagagcctca tacgaacgcc agaaggtaga tcgtaagagc
     1501 ggtgccagtg gatcgatacg tcgtgggtgg tgtgcccgaa cgagtgctgg cagcgtgttc
     1561 cagtgcaggg tcagatgcgt gtgaaaatcg atcaggttgc gagcagagac gccgcgcgca
     1621 cgagtgttca tcgctcgact ctccgtcagg ggaacggact ggttcagcga accggaccgt
     1681 agcacttgcg gacgatcttc tcgaccagag cgtcggataa gccaacgtag ttgcgcagtt
     1741 tggccttacg ctcgatgcgc tgcagtcgat tgacggcgat ctcttcgggc accttcccat
     1801 cgagtttctc gctggggata ttgtgtcgcc cctcatactt gagcaagcac cagtaggaga
     1861 ttttgacctt gttgatcagg ttgtaggttt cgagaaagtc ttctttggtt tcgcgtggga
     1921 acccgaccat gacgatcgag ttcatgctct tgaccctggt gttgtcgatc acgtgattga
     1981 cgacgcggat gtagtcctcg atcacgtagt tccggcccat gagcttgaga atgcgattcg
     2041 aaccggactg aaccggtgac ccgaacgagc ggattttccc tgactggaag acgggttcga
     2101 ggcgatcaat gtatttaatg agccagtcgg gctcgacata gtgcatatcg acaacaaaat
     2161 cgcctggtac ttttacgacc tcgttcagca ggtcgggtaa tgacgagccg atgtcctgtc
     2221 cccagcaccc catgtcggtg ccagtgaagg aaatggtctt gtaacctcgt cgtacagcgc
     2281 gttccaactg tggcatgatc tggtcgagac cggtgctctt gattttgccg cgcgcatgtt
     2341 tgatggcgca gaacgtgcag ttgccgagac acccgtttcc gccttggatg aaataccaat
     2401 cggtcatggg cagtgagtcg agccagttgg gcatttgtac aaacgggagg tatttctgaa
     2461 ctcgggtgac ggcattcttc agacgtcttc cgaccatgaa cgtgctgctc ttgagatact
     2521 cgtgatcatt cacgctgttg gtgacggaat cgcggaaaga ggtgcgcttc ggggcgatga
     2581 tcgtttcgat cttttccatg ttgagtggcg gaatgtcgac cacgtcaggg aattccttga
     2641 agacgtgagg cgcgatcttg ggataacagc cggagacgat gacctgcgcc ccgggcttgc
     2701 gattcgtgcg cgattccgtg atgttacgat aggcttcgtc ttcatactgc tggttgaacg
     2761 agcaggtcga aaccagaatc aggtccgcct cttcacggcg accgacctcg gtgaaatcgt
     2821 tcttaaccag atagtcgcgc agctgattcg tttccacgat gagttcggta caggcgtggg
     2881 ctgaggtaac gatgaagaat gtgttcatgg agaattccct cccgggttcg tgacgactct
     2941 cggacgtcac ttctcggcga tgccgatgac gaagtgacgt tgtggttcga cgtgaatggt
     3001 acgaaaacca gcgtcggtat acaatgtctg catctgctcg acagaatagg ggccatgggc
     3061 atcgtcgtgg tagccgtggc ggtcgcggaa atccacgatg aaggctgcgc cccccggttt
     3121 caatacccga tacacctcgg cccagatctt tgccggttct gacatttcgt gcatgcagtt
     3181 gcctgaaacg acccagtcga tcgattgatc aggcaatccc gtctgccctg cgtcgcagtt
     3241 caggacttcg attcgttcgc gcaccgcctg gcgagcggct atcgcctgca ggcgatcggt
     3301 catttcgatc gaggaatcaa ggcagtagat ggttccaccc ttgaggcgtc tggcaatggg
     3361 aagactgaag tttccggacc cggcaccgac atccagaact cgctcgttcc catgacgcac
     3421 tcgtcgaatc aactcgcgct tgaatgaggt ccagtagttc ccgaaacaga gaagctccag
     3481 catcttgcgg gtccaggtgc cgaaaaacca ggcatgaaat cgcccacggg gtccggtcca
     3541 ttctcccgga tgatgaatct gcatgctgcg cgtcatcctt tgcgatgaaa gtcaaatccc
     3601 agccgcactc atgggcagca tgagccaaac cggatgcccc attatatgcg aaaatcaggg
     3661 aatctgcgaa ggaaacgttg tgaaagggcg cgaaggagag tgccgtctca atcaagggtc
     3721 gtgtcagccg acccgcccga ttccggttca ggattgcgcc acatgggcac gagcatggtc
     3781 agaaaaaagg caccggagaa gaggccggca ccgatcagaa tcagtggtgc gacaagagac
     3841 ggctcaacga gggaacgctc tggattgtca gggtccactc ggacggaaaa gccgtctccg
     3901 agggtttgtc ggaaagcggt ggtcagtgac gcaacctcgg aaatcgaggg cctgacttta
     3961 cccaggtttt gccccgtgtt ccaggagccc gaataggagt ggctggcgac ctgatatcga
     4021 taggagaccg tgaaggagag cgaatagcgc gtctccggct gatcttcgtc cggcgggtgc
     4081 tcttcaacgg tggggtgaca accctcaacg gtaaccggca cgatatgcca gcgatacact
     4141 ctcatgactt gtacaaattc gcgaatgcca aagaaaagga agccacaggg gatcagaaga
     4201 atgaagagca gacaaaaaaa cagaaaagtg cgactgcggg gttgaattgc ggtggtcgac
     4261 ttcctgacca ttacgctgcg ggcgcctgtg gaggtttcgg gttgggatcg cgcgcggagg
     4321 tcttctcgag ttgtagctcg ggatacagtt ctttggcgca cggaggacag agacagtgcg
     4381 tgaactctgc ttcggttctc gacgagatgt agagttcgat gcgttgccag tatccctggt
     4441 cgtcgcgaat cttccggcaa tgggcacaga tgggcaagag gccgctcagc gtcttcactt
     4501 caccgatggc tctttccagt gatgcgacgt tgtccttcag ttgctgttgc gactcgacga
     4561 gttcctgttc gagacgctga ctgttgagca tgatgaatcc gacgacgaac ccaatttcca
     4621 gcaggggggc gaggaaaaag agcacggcgt tctgccaggt cggagtgaac atctggacct
     4681 ctgggtacac aaacagatac aggccgcgag agagaaagac gacgccggtg agagcgcaga
     4741 cgaatgccag tatcagactg gtctgccgcg gttgcagacg gttggctccg aactcatagg
     4801 tggtcagaaa caggacggtg ctgacgatca gagaataggt gagactgcgg gcagagaagt
     4861 ggtcgacggc aaggacgaaa tacagggcga ggccaagact gacgggcaac agcagatacc
     4921 aggcgggcga aatgggccga gacgtggtga agcgcctggc tccttcgaag cgca
//
LOCUS       DAORHD010000166         4943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17859_length_4943_cov_4.212152, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000166 DAORHD010000000
VERSION     DAORHD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4943
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17859_length_4943_cov_4.212152"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="PKO06_04895"
     CDS             complement(<1..1286)
                     /locus_tag="PKO06_04895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNV69015.1"
                     /translation="MRKYYTRIDRIAGNVATLKATGVGYEELALVEGSWGSSLAQVIR
                     AAGEDVTIQIFAGTRGVSTGDKVSFLGSPMRFSFSQDLFGRIFSGGGQPLDGRSPLTT
                     NLIEVGGPAVNPAIRLMPHRMIRTNIPMIDVFNTLVEGQKLPIFSIPGEPYNELLARI
                     ALQAEADVIILGGMGLKYDEYMFFRKKLEEGGAMSRSIMFVHTASDPVVECLLTPDLA
                     LAVAEQFALQGKRALVLLTDMTNFSNALKEIAITMEQIPSNRGYPGDLYSQLAARYEK
                     AVVFDNGGSVTLMAVTTMPGDDLTHPIPDNTGYITEGQFYLKGGWIEPFGSLSRLKQL
                     VNNKTRDDHRAIMDGCIQLYAKCRETREKRAMGFEMSGWDQKLLKYGQQFEDQIIDFA
                     ANVTLEGALDLCWRLLAANFEPRETGLRSSLIEKYWP"
     gene            complement(1279..3012)
                     /locus_tag="PKO06_04900"
     CDS             complement(1279..3012)
                     /locus_tag="PKO06_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNV69016.1"
                     /translation="MSLNGRIVTIYGNMVTAEADGHIRQNAVAFCTRGDGVRLKSEII
                     RVRGNRADMQVFELTRGLKVGDKVEFLDELLSLELGPGLLGQIYDGLQNPLPQLAEKV
                     GKFLQPGTYLPALDRQKKWKFTPVAKVGESVTAATNLGKVPEGIFDHFIMAPFDLKGE
                     WKLVSLKAAGDYTVEEEIAVIEQNGTKRSLNMINRWPVRIPLGIYKERLVPSQPLVTK
                     QRIIDTFFPIMLGGTYCIPGPFGAGKTVLQQITSRHAEIDIVIVAACGERAGEVVETL
                     VEFPHLKDPRTGRTLMERTIIICNTSSMPVAARESSVYTGVTLGEYYRQIGLNVLVLA
                     DSTSRWAQALREMSGRLEEIPGDEAFPAYLESSIARFYERGGQVRLPDGRLSSLTIGG
                     TVSPAGGNFEEPVTQGTLKVVGAFLGLSYARSYARRFPAIDPLESWSKYKSLIETEKV
                     REAHGFLRRGRSVNEMMQVVGEEGTSMDDYVVYLKAEFLDNVYLQQNSFDDVDAACPP
                     DRQKYAFEMILNVLRQKFPFKDKEAARNLFFSLTDQFRNWNYAPWQSPEMKKIEEQIQ
                     SLLNTGEMAHA"
     gene            complement(3009..3653)
                     /locus_tag="PKO06_04905"
     CDS             complement(3009..3653)
                     /locus_tag="PKO06_04905"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNV69017.1"
                     /translation="MYYLLTTLPQLPELGGAPAIPFAELQRIVQNDGYAAAVAVVDVL
                     ALERQVREAIDRRVLESATLDNDTDFPAIVQEALASDPVAMGEDEWLDRVWSAYFTHV
                     HAVGRKVHAPLLSKWAKFEPDLREHLAALRRGDKHTPGPFDMLMDIVGCNNPMDAEIM
                     LDTIRWRFLEKETPRFTFHIDELVAYALKHRLLERYVRLDRAKGTTILEEVTTL"
     gene            complement(3665..4267)
                     /locus_tag="PKO06_04910"
     CDS             complement(3665..4267)
                     /locus_tag="PKO06_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69018.1"
                     /translation="MGQDVAAFAKVLREEGIEAAKAEAGKILADARAKADEIVKEANT
                     AHRRKMEEANREIEQMRQRAEAEMKLVARDLILQVKKRVEEIAARLLSDQVEKALATP
                     EVVKNCLLEVLKTTKTGTEWELALGPTVGKSLAAAAAGDLFRQADAKGKLIEAIKAEG
                     FALKSPANNMVIEVTAESVSEAFRQLLSPELEKLLPAHNN"
     gene            4601..>4943
                     /locus_tag="PKO06_04915"
     CDS             4601..>4943
                     /locus_tag="PKO06_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV69019.1"
                     /translation="MTRRCRMLLALISALMLGFMLPGEAEAVVAGMKGVSVTSLSPRD
                     MAADVSSKSPIIIEFTGNVTPTFYQSVNVTMFHGPRTIDGELFYNQAAKQVMFKPKTP
                     LQEGQTYTAQVT"
BASE COUNT         1032 a         1469 c         1396 g         1046 t
ORIGIN      
        1 ggccagtatt tctcgatcaa tgaactgcgc agaccggtct cgcggggctc gaagttcgcc
       61 gccagcagtc gccagcagag atcgagcgca ccttcgagcg taacgttcgc ggcgaaatcg
      121 atgatctgat cttcgaactg ctgaccgtat ttgagcaact tctgatccca gcccgacatc
      181 tcgaaaccca tcgcgcgctt ctcgcgcgtt tcgcggcatt tcgcatacag ctgaatgcag
      241 ccatccatga tggcgcgatg gtcgtcgcgg gtcttattgt tgaccagctg tttcaatcga
      301 gacaatgaac cgaacggttc gatccagccg cctttcagat agaactggcc ttccgtgatg
      361 taccccgtgt tatcaggaat cggatgggtc agatcgtcac ccggcatggt cgtcaccgcc
      421 atcagcgtga ctgatccgcc gttgtcgaag accacggctt tttcgtaacg agccgcgagc
      481 tgagagtaca agtcgcccgg gtagccgcgg tttgatggaa tctgctccat cgtgatcgcg
      541 atttccttga gcgcgttcga aaagttggtc atgtcggtca gcagcaccag agcgcgcttg
      601 ccctgcaacg caaactgttc ggcgaccgcc agagccagat cgggagtcaa cagacactcg
      661 acgaccggat ctgacgcggt gtgcacgaac atgatcgagc gtgacatcgc gccgccttcc
      721 tcgagtttct tacggaagaa catgtactca tcgtatttga gacccatgcc gccgagaatg
      781 atgacatcgg cttcggcctg caacgcaatg cgggccagca gctcgttata cggttcgccg
      841 ggaatcgaga aaatcggcag cttctgccct tcgaccagcg tgttgaacac gtcgatcatc
      901 ggaatgttcg tccgaatcat gcggtgcggc atcagacgaa tggccggatt cacggcggga
      961 ccaccgactt cgatcagatt cgtcgtcagg ggtgaacgcc cgtcgagcgg ctgaccgccg
     1021 cccgaaaaaa tgcggccgaa gagatcctgt gagaaggaga aacgcatcgg cgaacccaga
     1081 aagctgacct tgtctccagt tgagacaccg cgggttccgg cgaaaatctg aatggtgacg
     1141 tcttctccgg cggcacgaat gacctgcgcc agagaagaac cccagctgcc ctcgaccagc
     1201 gcgagttctt cataaccgac tccggtggcc ttcaaggtcg ccacgttccc ggcaatacga
     1261 tcgatacggg tgtagtactt acgcatgggc catttccccc gtgttcaaga ggctctgaat
     1321 ctgctcttcg atcttcttca tctccgggct ctgccacgga gcgtagttcc agttgcggaa
     1381 ctggtcggtc aggctgaaaa acaggttgcg cgcggcttcc ttgtctttga agggaaactt
     1441 ctgccgcagc acgttcaaga tcatctcgaa cgcatacttc tgacgatcgg gcggacacgc
     1501 cgcatcgacg tcgtcgaacg agttctgctg cagatagacg ttatcgagga actccgcctt
     1561 cagatacacg acataatcgt ccatcgaggt gccttcttcg ccgacgacct gcatcatctc
     1621 gttgacgctg cgaccacggc gcaggaagcc atgagcctcg cggacctttt ccgtttcgat
     1681 caggctcttg tatttcgacc acgattcgag cggatcgatc gccggaaacc gacgcgcgta
     1741 ggaccgagcg tacgagagac cgagaaacgc gccgacgacc ttgagcgtgc cttgagtcac
     1801 cggttcttca aagtttccac cggcagggct gacggtaccg ccaatggtca gggacgacag
     1861 gcgtccgtcc ggcagacgga cctgaccacc gcgttcgtag aagcgggcaa tcgagctttc
     1921 gaggtaggcc gggaaagcct cgtcgccggg aatctcttcg aggcgaccgc tcatttcgcg
     1981 caaagcctgc gcccagcgcg aggttgagtc ggcaagaacc aggacattca acccgatctg
     2041 gcgatagtat tcgccgagag tcacgccggt atagaccgag ctttcgcggg ccgcgaccgg
     2101 cattgaagag gtattgcaga tgatgatggt gcgttccatc agcgtgcgtc ccgtgcgagg
     2161 gtctttcaga tgcgggaact cgaccagtgt ctcgacgact tcaccggcac gttcaccgca
     2221 cgcagcgacg atgacgatgt cgatttccgc gtggcggctg gtgatctgct gcagaacggt
     2281 cttgccggcg ccaaacgggc cgggaatgca gtaggttccg ccgagcatga tcgggaaaaa
     2341 ggtatcaatg atgcgctgtt tcgtcaccag cggctgggac ggaaccaggc gttccttgta
     2401 gatgccgagc ggaatgcgca ccggccagcg gttgatcatg ttcaacgagc gtttcgttcc
     2461 gttctgctcg atgacggcaa tttcttcttc gacggtatag tcaccggcgg ccttcagcga
     2521 caccagtttc cattcgccct tgagatcgaa cggcgccatg atgaagtggt cgaagatgcc
     2581 ttctgggact tttccgagat tcgtcgcagc ggtgacggac tcacccactt tggcgaccgg
     2641 agtgaacttc cactttttct gacggtcgag agccggcaga taggttccgg gttgcaggaa
     2701 cttgccgacc ttctccgcca gctgtggcag agggttctgc aaaccgtcgt agatctggcc
     2761 gagcagaccg ggccccagct cgagagagag cagctcgtcg agaaactcga ccttgtctcc
     2821 caccttcaag ccgcgggtca actcgaagac ctgcatgtcc gcgcgattgc cgcgcacacg
     2881 aatgatttca gatttcaagc gcaccccgtc gccgcgggta caaaaagcaa cggcattctg
     2941 acgaatgtgg ccgtcggctt ccgccgtgac catgttgccg taaatggtaa caatgcgtcc
     3001 gttgagactc acagggtggt gacctcctcg aggatagtgg tgcccttcgc ccgatcaagc
     3061 cgcacgtagc gctcgagcag acgatgcttc aaggcatagg caaccagttc atcaatatgg
     3121 aacgtgaacc gcggcgtctc cttttccaga aaccgccagc ggatggtgtc aagcatgatt
     3181 tccgcatcca tcgggttgtt gcagccgacg atatccatca gcatgtcgaa ggggccgggc
     3241 gtgtgtttgt cgccccggcg caaggcggcc aggtgttcgc gcagatcggg ttcgaacttc
     3301 gcccacttgc tcaaaagcgg cgcatggacc tttctgccga cggcatgcac atgcgtgaag
     3361 taggctgacc agacacgatc gagccattca tcttcgccca tggcaaccgg atctgacgcc
     3421 aaagcttcct ggacgatcgc agggaagtcc gtatcgttat cgagggtcgc gctctcgagc
     3481 acccgtcgat cgatcgcctc acgcacctga cgttcgagag ccaggacatc gactacggca
     3541 accgccgcgg cgtagccatc attctggacg atgcgctgca gctcggcgaa tggaatcgcg
     3601 ggagcaccgc ccagttcagg aagctggggc agtgtcgtta agagatagta caagctggac
     3661 acgatcagtt gttatgtgca ggaagcagct tttccagttc cggggagagc aactggcgga
     3721 acgcttcgct gaccgactcg gcagtcactt cgatcaccat gttgttcgcc ggactcttga
     3781 gcgcaaagcc ctcggccttg atcgcctcaa tcagcttgcc tttggcgtcg gcctgtcgga
     3841 acaggtcgcc cgccgccgca gcagccagag acttgccaac ggtcgggccc aacgcgagtt
     3901 cccattccgt gccggtcttg gtggtcttga gcacttcgag caggcagttc ttcacgacct
     3961 caggggtcgc cagggctttc tcaacctgat cgctcagcag acgtgcggca atctcttcga
     4021 cgcgcttctt cacctgcaga atcagatcgc gagccaccag tttcatttcg gcttccgcac
     4081 gctgccgcat ctgctcgatt tcacggttcg cctcttccat cttgcgacga tgggccgtat
     4141 tggcttcttt gacgatctca tcggccttgg cccgggcgtc agcgagaatc ttcccggctt
     4201 ccgccttggc ggcttcgatt ccttcttcac gcagaacctt ggcaaatgcc gctacatcct
     4261 gacccatggt ggcactcctt gaagtgaatc cgtctttacc gaactggtac cgaaacggaa
     4321 ctcattttct atcacatccg catgaacagt gcaatttatt tcacaaagtg catgtaccac
     4381 tgccaagatc attgatgctg tctgccagcc acttccgctc gacggcacga cgctgtgcca
     4441 gcgtatggga ctgcaggcaa cgcatttcag acccgagcgc accagatctg actcccggca
     4501 tccaggccat tcccgggaag ttccagcttg cccctcatca tgagcatcga aaatgccgat
     4561 agaggttacc aagatagttg cgtcctcgag gaggtaaacc atgacacgtc gttgccgaat
     4621 gctcctggcg ctgatctctg cgctcatgct ggggttcatg ctgcccggag aagcggaagc
     4681 cgttgttgcc ggaatgaagg gggtatccgt tacttccctg tcaccacgcg acatggcggc
     4741 agatgtaagc tcgaagagtc cgatcatcat cgagtttaca ggaaacgtca cgccgacttt
     4801 ctatcagtcg gtcaatgtca ccatgtttca cggaccgcga accattgatg gtgagctgtt
     4861 ctacaatcag gccgcgaaac aggtgatgtt caagcccaaa acacccctgc aggaaggtca
     4921 aacctatacg gcgcaggtta cat
//
LOCUS       DAORHD010000167         4934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17907_length_4934_cov_3.275876, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000167 DAORHD010000000
VERSION     DAORHD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17907_length_4934_cov_3.275876"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKO06_04920"
     CDS             <1..970
                     /locus_tag="PKO06_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69020.1"
                     /translation="EAQLLATMGVTDGDESKLTNGQKALLEMYRFAQNPRLIEQMKKG
                     FKEKITILVTDTNGHNSDTVRNDFWPCAAEGQIQFSDMYFDRSDAIPLARSVFIHEAA
                     HTLDKTWQEYEQPYGPDGAHYANELTKPKAAFMEGWAEFAQMYYSADDERNTREAVQK
                     IKIEGREAGQYTEVSPQELTGMQLLSVEGINALIFLDLSRSLPNGSDAVFDAFMATNQ
                     RDRSLATVLKHLAEKYPAQIGAIAQIVDKNTCGKLTGPELVQLLGNSANLREYLAQRE
                     EARRAAAAAAASATVKEELMTVGSPQGQPGSVTPAQVEGGNPYADY"
     gene            1134..>4934
                     /locus_tag="PKO06_04925"
     CDS             1134..>4934
                     /locus_tag="PKO06_04925"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69021.1"
                     /translation="MSRFLGGVVLPLLLMLIPAVILRQGSRWERQQLLRQQETQLRDT
                     AAAAGGKLQQAIRAAYWVEGSARRLVKVVDQRLSTTTRHPTARELGEAISDSLAEAYA
                     AGVPEPRLWAITVPAASAEAELCESEGLESFSARLMTRLLSEMAHDASGEGSELTTTL
                     WKQRLEQLFGYGCAEHFLERYRGRAFAVVFQGQGGMACWDFLSVGGRRVGFFLAISPL
                     QDDRSTLLASLVLSRWPEIAGTGAPLPILIPLPTVAAPAPAPLLHPALVGDETAVALI
                     RRVQSRLSLAPGIPWRDDYIRGLIRTGAKTPRQAGEHFLGRIGLPVDLIREELSLPNE
                     PYSGVLLPPVSRAGWLVLMVNRKPQVPVGFLTFLADLAWLWWLLAVLVALSQQALGNL
                     PVLSVRASPLLWLLGLGAIPLSHSVVVLAQWRIDEQQLLLANLREQLFRRLGQIESRF
                     LTFQQTRQQACREFFRQGVGGGGLGERLRQVQFQGAEARHVQKDIWEHFRDLGLPLTA
                     LLILGHDDYILRLFEPGLPPGVGTSLEIIVRGAMTNELHKQRPASLPAVRQGHHAPFI
                     MDRLSRNSDYGRQQLGQFVNWGAGSRRAQGYADWIKIGDECWYILYALWSSDTLVHGF
                     LTSPHEAAERRGPYWILARSGNSWVARPGDDVPEPLLARAQQLGERAGLIATEHEDRL
                     CAFHSSQSLPGYLLVTWAETTSIHVRIRRELLISLTVWTALALCIALGVSRVAIWLTE
                     PVLEMTESLKRVAGNDLSCHLDASYGGEFGDTAKSLNTMVGWLRERRNMSRFVSSEVL
                     EAVRERSSGEPAPVSRRRVAMLVSDIRAFTEMSERFPPQQVFDLLNEHLKRMTSIIQH
                     RGGMIDRCIGDAIQAVFPAATPEEALAAATAAAIDMRRSQREYHLERLRAGLFGYETG
                     IGIATGEVMAGIVGDRDSRLDFSLVGQPLLQAALLESQSKAGRDSRIIVSVQDAERLS
                     ADLTTLPLDGFADARELRLPSPTVESTRESHAEKALEQESRSVTAPGEERLKATWRWR
                     VVSLLLLWVFFPLLVLGFHTWSRYRQRQVAMDDWNRMLSRDQQRVRVMAGSRSLEEWW
                     LWRRLAQPTNALPGTASSTTNTELCLRHLQQELPDISWYLGNGDRVVASHGAPVGLTA
                     ERMDALMRALHDDLYLSGGSNGEDQNRAGNGIGLESFLRRDRGAQMCGRLLQVQTPIG
                     PRWLFWRPLRRDGEAWAPRSAVPFVPDRKGRERLGERSHYLGDVVLLLTERTLAEVKR
                     HRRRT"
BASE COUNT          937 a         1338 c         1607 g         1052 t
ORIGIN      
        1 tgaagcgcag ctcctcgcaa ccatgggagt cactgacggt gacgagtcga aactgacgaa
       61 tgggcagaaa gcattgctcg agatgtaccg ctttgcgcag aacccgcgtc tgatcgaaca
      121 gatgaagaaa gggttcaagg agaagatcac gattctggtg accgatacga atggtcacaa
      181 ttccgatacc gtacgaaatg atttctggcc gtgcgcagct gaagggcaga ttcagttcag
      241 tgacatgtac ttcgatcgat cagacgccat tcccctggca cgatcggtgt tcattcacga
      301 agccgcgcac acgcttgata agacctggca ggaatacgag caaccgtacg ggcccgatgg
      361 cgcgcattac gccaatgagc tgaccaagcc caaggcggcc ttcatggaag gctgggccga
      421 gtttgcgcaa atgtactact cggctgacga cgaacgcaac acgcgtgaag cagttcagaa
      481 gatcaagatt gaaggcagag aggctggtca gtataccgaa gtcagtccgc aggaactgac
      541 cggcatgcag ttactgagcg tcgaaggaat caatgcgttg atctttctcg acctgtcgcg
      601 ttcgttgccg aacggttctg atgccgtatt tgatgcgttc atggccacca atcagcgtga
      661 tcgttctctg gcgaccgtgc tgaagcatct ggccgagaag tatccggcac agatcggagc
      721 catcgctcag atcgttgaca agaacacctg tggcaagctg acgggtcctg agttggtaca
      781 gttgctgggc aattccgcga atctgcgcga gtatctggca cagcgtgaag aagcccggcg
      841 tgcggccgct gccgcagcgg cgtcggcgac ggtgaaagaa gagttgatga cggttggcag
      901 tccccagggg cagccgggtt ccgtgacccc tgcccaggtc gagggtggca atccatacgc
      961 cgactactga agtgaaggtt ctcaccgccg gtctcccgcc tgggatgacc ggcggtcttt
     1021 ttttaccatc cgagcaaaac gcgttaaaat gcgggcatca atcaagtgag ggtgaggcgc
     1081 agaagcgtgg tgtggatggg agtcattgag atgattgttc cgagagcgct cgcgtgagtc
     1141 gatttctggg cggggtggtg ctgccgctac tgctcatgct gattcccgcg gtgattctgc
     1201 ggcagggctc ccggtgggag cggcaacagc tgctgcgcca acaggaaacg cagttgcgtg
     1261 acacggcggc cgcggctggt ggaaaactgc agcaggccat acgggctgca tattgggtcg
     1321 agggttctgc ccgacgtctc gtgaaagtgg ttgaccagcg gctgagcaca acaacccgcc
     1381 atcctaccgc acgtgaactt ggcgaagcca tcagcgattc tctggccgag gcgtatgccg
     1441 ccggtgtgcc tgagcctcgc ctctgggcga tcaccgtacc agctgcttcg gcagaagcgg
     1501 agctgtgtga aagcgaaggg ctcgagtcgt tctctgctcg attgatgacc cggttgctga
     1561 gtgagatggc acacgatgcg tcgggagagg gttctgaact gaccaccacg ctctggaaac
     1621 aacgactgga gcagttgttt ggctatggat gcgccgagca ctttctggaa cgttatcgtg
     1681 gtcgggcgtt tgccgttgtg tttcagggcc aggggggcat ggcctgctgg gattttctgt
     1741 ctgtcggcgg cagacgggtc ggcttttttc tggcgatctc tccactgcag gacgaccgga
     1801 gcacacttct ggcgtcgctg gttctttcgc ggtggccgga gattgccggg accggtgcgc
     1861 ctttgcccat cctgatcccg ctcccgacgg tagcggcacc cgctcctgct cccctgttgc
     1921 acccggcact ggtgggagac gaaaccgccg ttgctttgat ccggcgggta cagtcacggc
     1981 tttcgctcgc gccaggaatt ccctggcgtg acgactatat tcgaggtttg attcgtacgg
     2041 gtgcaaaaac accaagacag gcaggggaac actttcttgg gagaataggt ctgccggttg
     2101 acctgattcg ggaagaactg tcgctgccca atgagccata ttcaggggtg ctgctgccac
     2161 cggtgtcgcg tgccggttgg ctggttctga tggtcaatcg gaaaccccag gtgcccgtcg
     2221 gctttctgac attcctggcc gatctagcct ggctgtggtg gctgctggcg gtgctggtcg
     2281 cgttgtcgca acaggcgctg ggaaatctgc cggtgctgag tgtgcgtgcc tctccgttgc
     2341 tctggttgtt gggacttggg gcgattcctc tcagccatag cgtcgtggtt ctggctcagt
     2401 ggcgtattga tgaacagcag ctgttactgg ccaatctccg cgaacagttg ttcaggcgac
     2461 ttggccagat tgaaagtcgt tttctgacgt ttcagcagac ccgacaacag gcctgtcgtg
     2521 agttcttccg gcagggggtg ggtgggggcg ggctcggtga acggctgcga caggttcagt
     2581 ttcagggggc tgaagcacgg cacgtgcaga aggatatctg ggaacacttt cgggacctgg
     2641 ggttgccgct gaccgcactg ctgatcctgg gacatgacga ttacatcctg cggctgttcg
     2701 aacctggcct gccacccgga gtcgggacca gtctcgaaat catcgtgcgt ggggccatga
     2761 cgaatgaatt gcataaacaa cgtcctgctt ctctgccggc agttcgtcag ggccatcatg
     2821 ctccattcat catggatcga ctctcgcgta actcggacta tggccgacag cagctgggac
     2881 agttcgtgaa ctggggcgcg ggcagtcgcc gggctcaggg atacgccgac tggatcaaga
     2941 tcggtgatga gtgttggtac attctctacg cgctctggag ctcggatacc ctggttcatg
     3001 gctttctgac cagcccccat gaggcggccg aacgccgcgg cccgtactgg atcctggcgc
     3061 gctccgggaa ttcctgggtc gccaggcctg gcgatgacgt gccagagcct ctgcttgccc
     3121 gggcgcagca actgggagag cgagcgggat tgattgccac tgaacacgaa gatcgcctct
     3181 gcgcttttca ttccagccaa tcgctgcccg gatatctgct ggtgacctgg gcagagacga
     3241 catcgatcca cgtgcggatc aggcgtgaac tgttgatcag tctgaccgtc tggaccgctc
     3301 tcgctctctg cattgcgctc ggcgtgagtc gcgttgccat ctggctgacc gagccggtac
     3361 tcgaaatgac cgagagcctg aaacgagtgg cgggaaacga tctttcctgc catctcgatg
     3421 ccagctacgg tggcgaattc ggcgatacgg cgaaatcgct gaatacgatg gttggctggc
     3481 tgcgcgagcg tcgcaacatg agtcggtttg tgtcgtccga agtcctggaa gcggttcgcg
     3541 agcgttcgag cggggagccg gcccctgttt ctcgccgtcg ggtggccatg ctggtctcgg
     3601 atatccgtgc ttttacggag atgtcagagc ggttcccgcc gcagcaggtc ttcgatctgc
     3661 tgaacgaaca tctgaaacgc atgaccagca ttattcaaca ccgtggcggc atgatcgacc
     3721 gatgtatcgg cgatgccatt caggcggtgt ttccggctgc cacccccgaa gaagcgctgg
     3781 ctgccgcaac cgccgcggcc attgatatga ggcgttccca gcgcgagtat cacctggaac
     3841 gtctgcgggc cggtctgttt ggctatgaga cggggatcgg aatcgcgacg ggagaggtca
     3901 tggccgggat agtcggtgac cgagactcga gactcgactt ctcactcgtc gggcagcccc
     3961 tgctgcaggc agccttgctc gaatcacagt caaaggcagg aagagactcg cgaatcattg
     4021 tatccgtcca ggatgccgag cgactatctg ctgatctgac gacgctgcca ctcgatggat
     4081 tcgcggatgc ccgcgagctt cgtctgccgt cgccgacggt tgaaagtaca agagaatcgc
     4141 atgcggaaaa ggcattggaa caggagagcc ggtcggtgac tgctccgggg gaagaacgac
     4201 tgaaggcgac ctggcggtgg cgtgtggtgt cgctgcttct gctgtgggtt ttcttcccgc
     4261 tgctcgttct cggctttcac acctggtcgc gatatcggca gcgacaggtg gcgatggatg
     4321 actggaatcg catgttgtcc cgggatcagc agagagtgcg ggtgatggcg gggagccgca
     4381 gtctcgagga atggtggctc tggcgacgat tggcgcaacc gacgaacgcg ttgccgggaa
     4441 ctgcctcatc tacgacgaat actgaactgt gtttacgtca tttgcagcag gaactgcctg
     4501 atatcagctg gtacttggga aacggagatc gtgtcgtcgc ctcacacggg gcgccggtcg
     4561 ggttaacggc ggagcgcatg gatgcgctga tgcgtgcgct gcatgacgat ctttatctga
     4621 gcggcggttc gaacggtgag gatcagaatc gcgccgggaa cggtattggc ctcgaatcct
     4681 ttttgcggcg tgatcgtgga gctcagatgt gcggacggct gcttcaggtt cagaccccaa
     4741 tcggcccacg ttggctgttc tggcgacccc tgcggcgaga tggtgaggca tgggcgccgc
     4801 gcagtgccgt tcccttcgtg cccgatcgta agggaagaga gcggctcggg gagcgctcgc
     4861 actatctggg tgacgtcgtg ctgcttctca cggagcgaac actcgcggaa gtgaaacgtc
     4921 atcgccgtcg tacg
//
LOCUS       DAORHD010000168         4924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_17949_length_4924_cov_3.704046, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000168 DAORHD010000000
VERSION     DAORHD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17949_length_4924_cov_3.704046"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2638)
                     /locus_tag="PKO06_04930"
     CDS             complement(<1..2638)
                     /locus_tag="PKO06_04930"
                     /inference="COORDINATES: protein motif:HMM:NF017548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HNV69022.1"
                     /translation="MRQTIFSLRFRQFFFCLVLIGMVLFNTGVEPALADIRNAKLTVD
                     LPGGATAGAKFNGGATFGLKATWEGDTPPYAATFKAGGDIGTSNSDAGSADFSVSAAA
                     LGPGAKSFSVSIIETSVPNAKPGTATGDKQADIDTTPLDLSVQIDSATKKVGKGGTVR
                     FTVTALNGKTFGVVPTAVVNPGGAAATPVNPIIPGSVVNYTYTVPNDAAGGNYTVQVT
                     GKDNTFPQTTANTGSGQDVFGVDVEGPGSPAIVSASVGNPTRAENITLSGTCSAETVS
                     VEVLDKGSSLSPALVVSPSGTNWQATVVSVSEGKHRFTAKSKDEFGNESVISSEMAIN
                     VDRTKPNKPVLDTPVKRTSNKFITISGKAATDNPTSTDSLVSTPVEVHLYRVGETATA
                     AASMNADASGNFSFSNIELKADNAGQNIFYAVAEDNARGSPGNMSDQSSTVTVYYDTD
                     KAVPSALLVSSVALASASLPFGDFLAPGTYNVQVTFSENMDRTVNPTISFLGAGGGTQ
                     SSSSGSWTASTTFLGQIIIPAGQGTTFDGRAQNIRVSGAKDEAGNTMDDYTQNNPFGI
                     DTTAPQTTMDSMDTIYVGSGTTSVTVQGSASDTTSGVGYVELWWEPFNNPTGTHATQS
                     IGLAPGSNRWVTVLDVAALGIGDGAYKLWAYAGDRARPNPNVETRQPTGYRLLIVDRN
                     VPQVERISFDDESVDLQPAGNPPPTVGSDVTKLTAFINKGSGSSTLVLTVPPMIFTLK
                     DPAGTAITGNYAPMGSSAVSFTFPKLTMNGTYSIEVQPIDAAGNKGTTATRSFILDLA
                     APDNVRFDPASGTIVNDTYPPIQADEVWAYIDDPSADYTKSTIEVMYNGVVVGSQKTA
                     STSLIWDLYGAVASHPTDQSGDG"
     gene            2912..>4924
                     /locus_tag="PKO06_04935"
     CDS             2912..>4924
                     /locus_tag="PKO06_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV69023.1"
                     /translation="MSQRFKLFGLLVFVGLSAGLVSLLTGCGGVNPTSSYTPTSNLPA
                     WALDQGGISGRVTIGSSTFGIAGALIEAAQAQAISDADGIFLMGPLPQGEYRVTIRAA
                     GYGPQVRTGVRVRSGLITDAINFQMSTETASATRDFSVVSLVPDFGTDGDEINVLGTG
                     FGSVPGKVTVAGKDAAVMDWNSKLDGIIRIRLPNEVETGVVKVIINGENSHEASPVTF
                     TGRPIALEVRPSGARPGQQVTIYGRNFHEIPSFNRVTLNGVNCTILPESNTRQLVILV
                     PSNAQTGILNVSIQSPQIQLDGISFATLTIPPELIYLTPQRSIPDVTLTLYGKNFGND
                     VNAVSIKLGDRKRLGPNDLLSFSNNKVTFKAPDSTVLPPGESVDVWLYVNDIPCLTPL
                     RYTAYNPALETLQTGQYGIYRFADKSNGNTLRLSKLAENERLAFLVSLCGDGTTDLAG
                     SFSYAITSRLGGNNAAVPTLPTASLAASKHNIRHSPSGKTIHDAGTSMRSWYKSSNLR
                     SSLRQQIRPSLDADAPASTTFWLVNFFSSDPSDPANDELATATLPATGTRCLVYVDNA
                     TDTVLTASNAENIATWFDNIYATIATACSDGISVPPEGNVDAQSRIVLFITPQINRGV
                     TSGLVTYGYFNPRDKNLGSVHSNGTEILYFWDQAFEDPANFRGIMAH"
BASE COUNT         1059 a         1467 c         1328 g         1070 t
ORIGIN      
        1 gcccgtcacc gctctgatca gtcggatgcg aggccacggc cccgtacaga tcccagatta
       61 ggctggttga agccgttttc tgactgccaa ccacgacgcc gttatacatc acctcaatgg
      121 tcgacttggt atagtcggcg gacgggtcgt cgatgtaggc ccacacttca tcggcttgaa
      181 tcggcggata cgtgtcgttc acgatcgttc cagacgctgg gtcgaagcgc acattatcgg
      241 gtgccgccag atctagaatg aacgatcggg tcgcggtcgt gcccttgttg ccggcggcat
      301 cgatgggctg cacttcgatc gaatacgttc cattcatggt cagtttcggg aaggtgaatg
      361 acacggcgct gctgcccatc ggggcatagt ttcccgtgat ggctgtccca gccggatctt
      421 tcaaggtgaa gatcatgggc ggaacggtga gaaccagagt tgaactgccg ctgcccttgt
      481 tgatgaacgc ggttaacttc gtgacatcac tgccaacggt gggcggcgga ttgccggccg
      541 gctgaagatc gaccgactcg tcatcgaaac tgattcgctc gacctgcgga acgttacgat
      601 caacgatcag cagacgatat ccggtcggct gccgagtctc gacgttgggg ttcgggcgtg
      661 cccggtcacc ggcatacgcc cagagcttgt aagcaccgtc gccgatcccg agagcagcga
      721 catccaacac cgtgacccat cgatttgatc caggagccag accaatggat tgagtcgcat
      781 gcgttcccgt cggattgttg aagggttccc accagagctc aacatacccg acacccgatg
      841 tggtgtcgct ggcagaaccc tgaacggtga ccgaagtcgt gccgctgcct acatagatgg
      901 tatccattga atccatcgtg gtctgcgggg ccgttgtgtc gataccgaac ggattgttct
      961 gggtgtagtc atccatcgtg ttcccggctt catccttcgc gccgctgact ctgatattct
     1021 gcgcgcggcc gtcaaaggtt gttccctgac cggcaggaat gatgatctgt ccgagaaagg
     1081 tcgtagaagc ggtccaggag cccgagctgc tgctctgcgt tccaccgcca gctccaagaa
     1141 agctgatggt cggatttacc gttcgatcca tgttttcact gaatgtgacc tggacgttat
     1201 acgtgccggg agccaggaaa tcaccgaagg gcagcgaagc actggccaag gccacggaac
     1261 tgacaagcag ggccgaggga acggctttgt cagtatcgta gtagacggtg acggtcgatg
     1321 actggtcgga catattgccg ggagatccgc gggcgttgtc ttcggcaacc gcatagaaga
     1381 tgttctgacc tgcattgtcc gctttcaatt caatgttact gaacgagaag ttcccactcg
     1441 catcagcatt cattgatgcc gccgcagttg ccgtttcacc gacccggtac aaatgcacct
     1501 caaccggagt cgacaccagc gaatcggtac tcgtgggatt atcggtcgca gccttccccg
     1561 agatggtaat gaacttgttc gacgtgcgct tcaccggcgt atccagcacc ggtttgttgg
     1621 gcttggtgcg atcgacgttg atggccattt ctgaagagat caccgattca ttgccaaact
     1681 cgtctttcga cttcgccgtg aaccgatgct tcccttcaga gacactgacg accgtcgcct
     1741 gccagttcgt tccactaggc gacaccacca gagccgggct cagcgaagaa cctttgtcga
     1801 gaacctcgac gctgacggtt tcggcgctgc aggtgccgga caaggtgatg ttttcggcac
     1861 gagtcggatt acctacggaa gcggacacga tcgcggggga cccgggacct tcgacatcaa
     1921 ccccgaatac atcctgaccg ctgccggtat tggcggtggt ttgcggaaag gtgttgtctt
     1981 tcccggtgac ctgcacggta tagttgccac cagcagcatc gttcggaacc gtatacgtgt
     2041 aattcacaac tgatccagga atgatcggat tgaccggagt cgccgcggca ccaccgggat
     2101 tcaccacggc ggttggaaca acaccgaagg ttttgccgtt gagagcagtc acggtgaaac
     2161 gcacggtgcc ccccttgccc accttcttgg tcgcggaatc aatctggacg gacagatcga
     2221 gaggcgtggt atcgatatcc gcctgcttgt ccccggttgc cgtccccggt ttcgcgttgg
     2281 gaacggaagt ctcgataatg ctgaccgaga agctcttcgc gccaggccca agagccgcgg
     2341 cagaaacgga aaaatccgcc gaaccagcgt ctgagttcga ggtgccgatg tctccgccgg
     2401 ctttgaaggt ggcggcgtac gggggggtgt ctccctccca cgtcgctttc aagccaaacg
     2461 tggctccacc attgaatttt gcgccggccg tcgccccacc tggcagatca actgtcagtt
     2521 tggcgttccg gatgtcagcc agcgcaggct ctacgcccgt gttgaacagc accatgccaa
     2581 tcaacaccag gcagaagaaa aactggcgaa agcgaagtga gaagatcgtt tgcctcatgg
     2641 tcaatctcct atcggggcaa aggacgccgc cgaaaatgac tttttattcc atcttaactt
     2701 cggcaaacac aaccccaaac ttgacaggcg actcggctca agaagaaatt tttcgccgtt
     2761 ttttcccgac gctgaccgtg gacaaaatat ttcccccgga aaatggtccg cgcctctcca
     2821 gatttgcctg atttctccga tatacagtaa cagggtctct ggttgagaga tcctctctgg
     2881 tcttcgagtt ccacacatcg ggataaccac cgtgtctcaa cgattcaaac ttttcggtct
     2941 cctcgttttc gtcggcctgt ccgcaggtct cgtttccctg ctgaccggtt gcggcggagt
     3001 gaatcccacg tcctcctata cccccacctc caaccttccc gcctgggcgc tcgatcaggg
     3061 aggaatctcg ggtcgggtga cgatcggcag ctcaaccttc ggcattgccg gggctctgat
     3121 cgaagccgcg caggcacagg caatcagtga tgctgacggc atcttcctga tgggccctct
     3181 gcctcaaggc gagtaccggg tgactattcg agcggcagga tatgggccac aggttcgtac
     3241 cggagtccga gttcgatcgg gcctgatcac ggatgcgatc aactttcaaa tgagcacaga
     3301 aaccgcatcg gctacacgag atttttccgt tgtatctctc gtgcccgact tcggtaccga
     3361 cggcgatgaa atcaatgtct tgggcacagg cttcggaagc gttccaggaa aagtcaccgt
     3421 cgccggcaaa gacgctgccg tgatggactg gaacagcaaa ctggacggaa tcatccggat
     3481 tcgcctgccg aacgaggtag aaaccggggt ggtcaaggtc atcatcaacg gcgaaaactc
     3541 gcacgaggct tccccagtga cattcacggg gcgtccgatt gctctcgaag tccgaccttc
     3601 cggcgcacgc cccgggcaac aagtcacgat atacgggcgt aactttcacg aaattccctc
     3661 cttcaatcgg gtcacgctca acggggtgaa ctgcacgatt cttcccgagt caaacacgcg
     3721 acaactcgtg atactggtac ccagcaatgc tcagaccggt atcctgaatg tcagtattca
     3781 gtcgcctcag attcaacttg acggcatttc cttcgcgacg ctgaccattc cccccgaact
     3841 gatctatctg acgccgcaac gatccattcc tgatgtcact ctgacactgt atgggaaaaa
     3901 cttcggcaat gatgtcaacg ccgtcagtat caaacttggt gaccgcaagc gactgggtcc
     3961 gaatgatctg ctcagcttct cgaataacaa ggtcaccttc aaggcacctg atagcacggt
     4021 tctgccgcca ggggaaagcg tcgacgtctg gctatacgtc aacgacattc cctgcctgac
     4081 gccgctcaga tataccgcat acaatcccgc tctcgaaact cttcagactg gacaatacgg
     4141 aatctatcga tttgccgaca agtcgaacgg caacacgctt cggctgtcga aactcgccga
     4201 gaatgaacgt ctggcattcc tggtttcctt gtgtggagat ggcacaactg acctcgcggg
     4261 cagcttctca tacgcgatca cttctcgtct gggcgggaac aacgccgcgg ttccgactct
     4321 gcccaccgcc tctcttgccg cctcaaagca caatatcaga cactcgccct ccggaaagac
     4381 cattcacgat gcaggaacaa gcatgcgttc ctggtacaag tcctccaacc ttcgatcctc
     4441 gctccggcaa cagattcgac catcgctcga cgcggacgcc ccggcatcga ccacattctg
     4501 gctggtgaac tttttcagtt ccgatccgtc cgatcccgca aacgacgaac tggcaaccgc
     4561 gactcttccg gccaccggca cacgctgtct ggtctacgtc gataacgcca ccgacactgt
     4621 tctgaccgcc tcaaatgccg aaaacatcgc cacctggttc gataacatct atgccacgat
     4681 cgccaccgcc tgcagcgatg gcatcagcgt gccccccgag gggaacgtcg atgcgcagtc
     4741 gcgtatcgta ttgttcatca ctccgcagat caatcgtgga gtgacttcag ggttggtgac
     4801 ctatgggtat ttcaacccga gagacaagaa cctcggttcc gttcattcca acggcaccga
     4861 gattctgtat ttctgggacc aagccttcga ggatccggcc aactttcgcg gtatcatggc
     4921 acac
//
LOCUS       DAORHD010000169         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18032_length_4910_cov_3.752626, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000169 DAORHD010000000
VERSION     DAORHD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18032_length_4910_cov_3.752626"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..571
                     /locus_tag="PKO06_04940"
     CDS             47..571
                     /locus_tag="PKO06_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69024.1"
                     /translation="MKNRGFTSIELLVVAAILAMVIAIGMQLFFSRNQAEQSLMGKLT
                     LQSDARKAADMIIARVREGAEIIRPFLGETRNYMVYLDGVNNTCMIYMTEDVENTQKF
                     KRPLYKLISYTHNYTGSGFQPMQEKVLGSSLQRVAFTTISPNSVQVTATIANEKSEFQ
                     FVTHAGLLNFGGAK"
     gene            568..2133
                     /locus_tag="PKO06_04945"
     CDS             568..2133
                     /locus_tag="PKO06_04945"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69025.1"
                     /translation="MKIYSATRGFTLPELLVGLGIMSFLAVAFFVFLQSSSRQSAFSA
                     EHFTGVLLAQKVVEDVAEEATTNPHAFLALGMSGGTAPKTPIIEGQSTFFSFLEDRYP
                     PLGKIDPAGEGTIDANYGPLYGQVKDFTLGVSGNALTPAGSIPENQHLSEARFDFSWK
                     AKAGTGQYSSRCLFFTPISAKPVSNVVTVNPNEVDAEICKRFFRSPGGDLTQLTTTYG
                     GDRETIRAIGTICVVTQAFTQSVTTKTLLQKQATLQSQVAVFGSNQSDTAYQARRSLA
                     ETTYEIAKLSFQVCHALQPSIDRLQNRLTQSQLGQVLWTYPNRYCYGLQALDRIYKSF
                     VTCLPLAAGHYRRLLDFPQAIARGPREFQDIVSRVVMLERVTTMVPELTSAMTTYRRT
                     LEDFRTFSQGRNPFLNRFVMQEKQFALTSASLADKHPHLAPVKSIAADSMVRFQNSLR
                     DLPPSPLNPFSPNANPSANTGGSTGTAGPGPTTSTGSQPPTSSGANPNNTGSGNGIDP
                     NATGKDPAEDGRN"
     gene            complement(2159..3214)
                     /locus_tag="PKO06_04950"
     CDS             complement(2159..3214)
                     /locus_tag="PKO06_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNV69026.1"
                     /translation="MNIFAMVRLLFSGSKSSVPPDLTLMTCPTCGNRQQEDPAEKKFV
                     CKSCGYTNPLLYPSPSRHRVNWFQLRPALDLSFLFLGILSATIGLKSFLLPNQLIDGG
                     VTGISMLAARITGIDLSWLIALINLPFMYAAWTQVGRGFAIRCALSIIGLSFMLGLIS
                     LPVLTRDHLLDAVFGGFFLGAGIGLSIRGGGVLDGTEVMALMISKRFPATIGDVILVF
                     NILIFSAGSLVLPIESVMYSILTYVSASKTVDFLMHGIEPFNGILIVSRFHQDIRQAI
                     VNDLGRGVTILQGKGGYSAREQEVLFCIITRLEIPKIRSIVHEIDPTAFLIIAPVTEV
                     SGGMIRKVLDTTPPVHT"
     gene            complement(3341..>4910)
                     /locus_tag="PKO06_04955"
     CDS             complement(3341..>4910)
                     /locus_tag="PKO06_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69027.1"
                     /translation="LNSAAVSGGVDYQGVYTPSGLKCQQDFGRELSTPLAVLFRTLGP
                     SLLHAANPELSAGSKIPAGLFEMSQTFMNEYMEMDSFGYYLGNPNRLCRTPYGNVQTW
                     YQIRFLRPDVKQLAHGVLINYGNYSLLRPSLDWFMKGVCYRLSDPEKYQLDLYLLRYH
                     NLINRTVAQETSSFQTPDSAYSTCRTLAESSLKHLVPRSSSHPDIHGQAEYLAQPLTE
                     DFAIGAARASPRQSLSRRQTLFVIVLLLSLCVFLYSLISTTTWYLLRPLKAFLQATAQ
                     AAQGVFTWRLDLRTSDECETMATELNQMAVKLVERQRMTRFVSDDMVKAIAEKEEQAI
                     RPGGQAITTTVLVSDIRSFTTLSEQHPPEAIVAALNEYFTTMEEAIQAEQGIIVQMIG
                     DAIVAHFPTPSADLSVSVPRGIRAALAMRRALREYNSERLKKGLFCLRTGIGIAAGEV
                     LSAVVGSATGRLGYTLIGTVVDRAVDLETFSKQGHSSGIMIDHSLVPYDHDGLSLLPT
                     SDKQACEISLDDPS"
BASE COUNT         1138 a         1302 c         1453 g         1017 t
ORIGIN      
        1 aagaaccgag ggttcacctc gatcgagttg cttgttgata acatctgtga agaaccgagg
       61 gttcacctcg atcgagttgc ttgttgtggc agccattctc gccatggtga ttgccatcgg
      121 catgcagttg ttcttcagcc gcaatcaggc cgaacagagc ctgatgggaa agttgacctt
      181 gcagagtgat gcacgtaaag ccgctgatat gatcattgcc cgagtgcgcg agggggccga
      241 gatcattcgc ccatttctcg gcgagacgcg gaactacatg gtgtacctcg atggggtgaa
      301 caacacctgc atgatttaca tgaccgagga tgtcgagaat acgcagaaat tcaaaaggcc
      361 cttgtacaag ctcatttcgt atacgcataa ttacaccggg tcaggattcc aacccatgca
      421 ggaaaaggtc ttgggaagct cgctacagcg tgtcgcgttc accacgattt caccgaacag
      481 cgttcaggtg accgcgacga ttgcgaatga aaagtccgag ttccagttcg tcacacacgc
      541 gggattgctg aattttgggg gggcaaaatg aagatttact ctgctacccg gggctttacg
      601 ttgcctgaac tgctggttgg gcttggaatc atgagcttcc ttgcggtggc gttttttgtg
      661 tttctgcagt cgagtagtcg ccagagtgct ttcagtgccg aacactttac gggggtgctg
      721 ctggcacaga aagtggtcga ggatgttgcc gaggaggcga cgaccaatcc gcatgcgttt
      781 ctggctctgg gaatgagcgg aggaacggct cccaagaccc cgatcatcga agggcagtcg
      841 acgttcttct cctttctgga agaccgctat cctcccctgg gtaaaatcga tccggccggc
      901 gaaggaacga tcgatgcgaa ctatggtccg ctctatgggc aggtcaaaga ctttacgctc
      961 ggggtttcgg gtaacgccct gacgcctgcg ggcagtattc cggaaaacca gcacctgagc
     1021 gaggcccggt tcgacttttc ctggaaagcc aaggcgggca cgggacagta ttcctctcgc
     1081 tgtctgtttt tcacgccgat ctcggcaaaa cctgtctcaa atgtggtcac ggtcaatccg
     1141 aacgaggtcg acgccgaaat ctgcaaacga ttcttccgtt caccgggtgg cgatctgacg
     1201 cagttgacga ccacctacgg cggcgaccgt gaaaccatcc gcgccatcgg aacgatctgc
     1261 gtggtcacgc aggcattcac gcaatccgtg acgacgaaga ccctgctcca gaaacaggcg
     1321 actctgcaga gccaggtggc ggtctttggt tcaaatcaga gtgatacggc gtatcaggca
     1381 cgacggtctc tcgccgagac gacctatgag atcgcgaaac tcagctttca ggtctgccat
     1441 gctctgcaac catcgattga tcgtttgcag aatcgcctga ctcaaagtca gcttggacaa
     1501 gttctttgga cgtatcccaa ccggtactgt tatgggttgc aggcacttga ccgaatctat
     1561 aagagctttg tgacctgtct gccgttagcc gccgggcact accgtcgcct gctcgatttt
     1621 ccgcaggcga ttgcccgcgg cccgcgcgag tttcaagaca tcgtttcgcg cgtggtgatg
     1681 ctcgaacgtg tgaccacgat ggttcctgag ttgaccagtg cgatgaccac gtaccgccga
     1741 acgctcgagg attttcgcac cttctcgcag ggtcgcaatc cgttcctgaa tcgatttgtc
     1801 atgcaggaaa agcagtttgc gctgacgtct gcttcactgg cagacaagca tccacatcta
     1861 gcgccggtca agagcattgc tgccgacagc atggtacggt ttcagaattc cctgcgtgat
     1921 ctaccaccca gcccgctgaa ccccttttcc cccaatgcga acccgagcgc caacacaggt
     1981 ggttcgacag ggacagccgg gcccggaccc acgacttcga cgggatctca accaccgacc
     2041 tcttctggag cgaacccaaa caataccggc agtggcaacg gcatcgatcc gaatgcgact
     2101 ggcaaggatc cggctgagga cggtcgaaac tgattcgttc ggttcgtcgg tgcgcgaatc
     2161 aggtatggac cggcggtgtc gtatccagca ccttgcggat catgccgccg ctgacctcgg
     2221 tgacgggggc gatgatgaga aacgccgtcg gatcaatctc gtgaacgatg gatcggatct
     2281 tggggatctc gagacgggtg atgatgcaga agagcacttc ctgctcgcgc gcagagtagc
     2341 cgcctttccc ctgcaggatg gtgacacccc ggccgagatc gttcacgatg gcttgtcgaa
     2401 tatcctggtg aaatcgtgag acgatgagaa tgccgttaaa cggctcgatc ccgtgcatga
     2461 gaaagtcgac ggtcttcgag gctgaaacgt aggtgagaat cgaatacatg accgactcga
     2521 tcggcagcac cagacttccg gctgagaaga tcaggatatt gaaaacgagg atgacatcgc
     2581 cgatcgtcgc cgggaaacgc ttgctgatca tcagggccat gacttccgta ccatcgagca
     2641 cgccgccacc gcgaatcgag aggcctattc ccgcgccgag aaagaagccg ccgaagacgg
     2701 catcgagcaa gtgatcgcgc gtcaggaccg gcagagagat gagacccagc atgaatgaca
     2761 agccgatgat cgagagagcg caccgaatgg cgaacccgcg tccgacctga gtccaggcgg
     2821 cgtacatgaa agggagattg atcagcgcga tcagccacga cagatcgatg ccggtgattc
     2881 tggccgccag catcgagatg ccagtgacgc cgccatcgat cagttgattg gggaggagaa
     2941 aacttttaag gccgatggtg gcggaaagaa tgccgagaaa caagaagctg agatccagcg
     3001 ctgggcgcag ttggaaccag ttgactcggt ggcgactcgg tgatggataa agaagcggat
     3061 tcgtgtaacc acaggacttg cagacgaact ttttctcggc tggatcttct tgttgacggt
     3121 tgccacaggt tgggcaggtc atcagtgtca gatcgggcgg gaccgatgac tttgagccgc
     3181 tgaagagcag gcgaaccatg gcgaaaatat tcaaagacgc ttctcctgcc gggaagagat
     3241 tgatccctct tgtgccaatc cgagatgaat cgacggcacc agtgagtgat ccaatctaac
     3301 agtatcagat tcagaacgga tttccaagcc ggcagagcgt tcaggaggga tcgtcgagcg
     3361 agatctcgca ggcttgtttg tccgaggtgg gaagcagaga aagaccgtca tgatcatagg
     3421 gaacaagaga gtgatcgatc atgattccgg aagagtgacc ctgcttcgaa aacgtctcga
     3481 ggtcgacggc acggtcgacg acggtgccga tgagcgtata tcccaggcgg ccggtcgcgg
     3541 agccgacgac cgcggaaagg acttctcccg cagcaatacc aatgcctgtt cgaaggcaga
     3601 acaagccctt cttcaatctc tctgagttgt actcccgcag ggcgcggcgc atcgccagag
     3661 cggcacgtat tcctcgcggg acggaaaccg acaggtcggc cgatggggtg ggaaagtgag
     3721 cgacgatggc gtcgccaatc atctggacga tgatgccctg ctctgcttga atggcttctt
     3781 ccatggttgt gaaatactcg ttcagcgcgg cgacgatggc ttcaggcgga tgctgttcgg
     3841 aaagtgtcgt gaagctgcga atgtccgaga cgagaaccgt ggtggtgatg gcctgaccgc
     3901 caggacgaat ggcttgttcc tccttttccg cgattgcctt caccatgtcg tctgaaacga
     3961 atctcgtcat ccgttgtcgc tcgaccaact tgaccgccat ctgattgagc tccgtcgcca
     4021 tggtttcaca ttcgtcactg gtgcgcagat cgaggcgcca ggtgaagacg ccttgagcgg
     4081 cctgagccgt ggcctgaaga aacgctttca acgggcgaag cagataccag gtggtcgtac
     4141 tgatgagcga atagagaaac acgcacagag acagcagaag aacgatgacg aacagagtct
     4201 gtcgacgcga taatgactgc cgcgggctgg cacgagcggc tccgatcgcg aagtcttcgg
     4261 tgagtggttg tgccagatac tcggcttggc catgaatgtc cgggtgagaa gaactgcgcg
     4321 gaaccaagtg cttcaggctg ctttcggcga gcgtgcggca cgtcgagtag gcggagtctg
     4381 gggtctggaa cgacgaagtc tcctgtgcca ctgttcggtt gatcagattg tggtagcgca
     4441 gaagatacag gtcgagctga tacttttccg gatcagataa ccgatagcag acgcctttca
     4501 tgaaccagtc gagcgaaggg cgaagtaagg agtagttgcc gtagttaatg agaactccgt
     4561 gcgccagctg ctttacgtca ggtcgaagaa aacggatctg ataccaggtt tgtacatttc
     4621 catacggggt tcgacacagg cgattcgggt tgcctagata gtacccgaat gagtccatct
     4681 ccatatattc gttcataaag gtctgcgaca tttcgaacaa cccggcggga atcttgctgc
     4741 cagcgctgag ttccggattt gctgcatgca ggagggacgg acccaaagtg cgaaaaagca
     4801 ctgccagagg ggtggaaagc tctcggccaa aatcctgctg gcacttcaga cccgaaggcg
     4861 tatacacccc ctgataatcg accccccccg agacagcagc cgaattcagg
//
LOCUS       DAORHD010000170         4907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18048_length_4907_cov_3.482688, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000170 DAORHD010000000
VERSION     DAORHD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18048_length_4907_cov_3.482688"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PKO06_04960"
     CDS             <1..492
                     /locus_tag="PKO06_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNV69028.1"
                     /translation="LSPQGKPYLPGRELSFSLAHSGQLVCLACGAVPRIGIDIEDCGR
                     PVQAAAIVSRSFVSAEQDRWQQLPESERASAFFRIWTRKEAYLKGTEEGIAGLARVEV
                     DQRCGVRRPWLSYDGVAAVTANWWSAEAEPIPGCLVTVAVDGPESELTWNWLPASPED
                     WPA"
     gene            complement(567..1925)
                     /locus_tag="PKO06_04965"
     CDS             complement(567..1925)
                     /locus_tag="PKO06_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69029.1"
                     /translation="MLRRTLFLTLFALIVACVTPAQAEDVNDLLTAMITAYSQSSKGV
                     QDEAFLEKVKAIDEHLREKIEKDHDLAPFRTVITAAQEMKSRLAARKMFEVLLDRVFK
                     RLQFTKAQQDITDADLKKEMDAMVASIEAYLDPNLPDDDTVDRASLETQRKRLREKIK
                     AAHAAILSKEGEKRITVQVVSQDGGEGKLKPSREANLLAKLKTVIPDPGKMTVTVTIK
                     RDDLHDIRVFVRDFVGPNGFSVVEGELDFVDVTAAALDDGNATELDNVLGDAHFRRYR
                     CVQTDEQLNIMPQAGNAAYEMGTMAPGQSWKMTFKKVDGISTVMKAMEGHEEVAILNA
                     KTAINDKALEFKNNGSRNLMYLQGTDVTGLVSVKALGYLTPKDGIVTFRDVLLVVDDG
                     VASDLTKFVGEQGINFFLKRASENLRKGLPFTPVLENVSCERNDTGNMVYIFTGRGKN
                     AL"
     gene            2033..3385
                     /locus_tag="PKO06_04970"
     CDS             2033..3385
                     /locus_tag="PKO06_04970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69030.1"
                     /translation="MGNLSIGLIKPSLGARFGRPYRTPAALEPLVFALIAGATSADVH
                     LELFDERYEAVPFDRPFSAVVLTVETYTARRSYQIADEFRRRGVKVILGGFHPTLLPD
                     EAAGHADAVAVGEVEGVWHQILGDLKAGRLADRYHATEPRTPFHLDRRLLSKYRYLPL
                     TLVETSRGCVHDCHFCSVRRFYGDRVRHRPIAEVVAELRTLDRSFIFFVDDNIAADPE
                     HAMNLFQALRPLGLRWISQASLATARRPGFLDAMAESGCFAVILGLESLSPTNLQRMN
                     KDWTLKLGTLESLLVEYRRRGILVYGTFVFGYDDDTPAVIEDTVDFTIAQRLFMANFN
                     ILQPFPGTRVYEELAADRRLLYERWWLDAEYRWERPAFRPRRMTVDELAAAVANARQR
                     FGSVGNLLRRACDQWANVADPFRAAVFLASNVVSRLDIKRKTGLRLGFPEPEAMEGTR
                     "
     gene            3382..4710
                     /locus_tag="PKO06_04975"
     CDS             3382..4710
                     /locus_tag="PKO06_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69031.1"
                     /translation="MRVTFIKPNMGLSNGRPYYDRGRMEPLTFAVLAGQLPGDVECRL
                     YDDRFERIPYHEPTDLVCLNVEIYTARRAYEIADRYRARGVKVVLGGYHVTMIPDEAA
                     LHADAIMIGDAEGQFARLVADAAAGRLQAVYRGEFPPSLTGLRTRWEIFNGKSYLPLR
                     LTQFSRGCVNHCRFCATGTIYRRVHCTRPPAEVIDELAQQPQPFVFFVDDNIIGNVDA
                     AKELFRRLIPLRRRWIGQASLTFTEDPELMDLMKRSGCAGLVVGFESVTPENIRQMDK
                     NCNMNLDSYAPVIEKIRRVGFQLWAAFLIGYDWDDWTTIHKTVQWAIDQRFCFAAFNI
                     LSPYPSTPLYEEMQTQGRLLYEKWWLDDRYHFGDCVFKPARMTPDELTEACFWARRRF
                     NHPLSIIKRAFDFQTNMKDLWSLATYVAYNPLFRRELFKKHGMVLGYQKR"
BASE COUNT          918 a         1387 c         1519 g         1083 t
ORIGIN      
        1 ctttcaccgc agggcaagcc gtatctgccc gggcgcgaac tttcattcag tctggcgcat
       61 tccgggcaac tggtgtgcct tgcctgtgga gcggttccgc gcatcggtat tgacatcgaa
      121 gactgcgggc gaccggtaca ggcggcggcg atcgtgagtc gctccttcgt ttctgccgag
      181 caggaccggt ggcagcaact gccggagtcc gagcgagcga gcgcattctt ccgcatctgg
      241 acaaggaaag aagcctatct gaagggaacc gaagagggaa ttgccgggtt ggcgcgcgtc
      301 gaggtcgatc agcggtgcgg ggtgcgacgt ccgtggctgt cgtatgatgg ggtcgccgcc
      361 gtcaccgcga actggtggag tgcagaagca gagccgattc cggggtgtct ggtgacggtc
      421 gcggtcgatg gaccggagtc ggagctgacg tggaactggc tgcccgcatc gcccgaagac
      481 tggccggctt aacgaatcag gggaaaacaa aaccgccgag cggtacagga ccgtcggcgg
      541 ttcgtggaat cgaggctcga tcgacgttag agcgcgtttt tcccgcgacc ggtgaaaatg
      601 tagaccatgt tcccggtgtc gttgcgttcg cacgagacgt tttccagcac cggcgtgaag
      661 ggcagaccct tgcgcaggtt ttcagaagcg cgcttgagga agaagttgat cccctgctcg
      721 ccgacgaact tggtcagatc tgaagcaacg ccgtcatcga cgaccagcaa cacgtcgcgg
      781 aaggtgacga tgccatcctt cggggtgaga taaccgagcg ctttcaccga taccaggccg
      841 gtgacatcgg ttccctgcag gtacatgaga ttgcgtgacc cgttattctt gaattcgagg
      901 gccttgtcgt tgatcgcggt cttggcgttc agaatcgcga cttcctcgtg gccttccatg
      961 gctttcatga ccgttgagat cccgtcgacc tttttgaacg tcatcttcca gctctgcccg
     1021 ggggccatcg tacccatttc gtaggcggcg tttccggcct ggggcatgat gttgagctgt
     1081 tcgtcggtct gtacacagcg atagcgacgg aaatgcgcgt cacccagcac gttgtcgagt
     1141 tcagtggcgt tgccgtcatc gagcgcagca gcggtcacat cgacgaagtc gagttcgcct
     1201 tcgactacag agaacccgtt cgggcccacg aaatctcgta cgaatacccg aatgtcatgc
     1261 agatcgtcgc gcttgatcgt gacggtgacg gtcatttttc cggggtcggg aatgacggtc
     1321 ttcagcttgg cgagcagatt cgcttcacga gaaggcttca gcttgccttc cccaccatct
     1381 tgcgagacca cctgaaccgt gattctcttc tcgccctctt tgctcagaat cgcggcgtgc
     1441 gcggccttga tcttctcgcg cagacgtttg cgctgggtct cgagactggc gcggtcgacg
     1501 gtgtcgtcgt caggcaggtt tgggtcgaga taggcttcga tggaagcaac catggcatcc
     1561 atctctttct tgagatcggc gtcggtgatg tcctgctgcg ctttggtgaa ctgcaggcgc
     1621 ttgaacacgc gatcgagcag aacctcgaac atcttgcgcg cggccaggcg acttttcatt
     1681 tcctgagccg cggtaatgac ggttctgaaa ggcgccagat cgtggtcttt ctcgatcttc
     1741 tcgcgcaggt gttcgtcgat tgccttgact ttttccagaa aggcttcgtc ctgaaccccc
     1801 ttgctgctct gcgaataggc agtgatcatg gcggtcagca ggtcgttgac gtcctcagcc
     1861 tgtgccggcg tgacgcaggc aacgatcagg gcaaacaagg tcaggaacaa tgtccgtcgc
     1921 agcatggaac cctcccgagg caaaagtaat gaattccgaa ctctggtgaa tgatactacc
     1981 acaaggtcgt gtataatcag cgaacggaac tttgcggaag ggacgcacat tcatggggaa
     2041 tctgtcgatc gggctgatca agccgagcct gggcgctcgc ttcggtcgac cgtatcgcac
     2101 cccggcagcg ctcgagccgc tggtgtttgc tctgattgcc ggcgcgactt ccgccgatgt
     2161 ccacctggag ctcttcgacg aacgatatga agcggtaccg ttcgatcgac ccttttccgc
     2221 ggtggtgctg acggtcgaga cgtacaccgc ccggcggtcg tatcagatcg ccgatgagtt
     2281 ccgccgccgc ggagtcaaag tcattctcgg cgggtttcat ccgacgctgt tgccggacga
     2341 ggcggccggt catgcagatg cggtcgctgt cggcgaggtc gagggagtct ggcatcagat
     2401 cctgggcgac ctgaaggcgg ggcggttggc cgatcgttat cacgcgactg aaccacgtac
     2461 cccctttcac ctcgaccgtc ggctgctgtc aaaataccga tatttgcctc tgaccctggt
     2521 ggagaccagc cgtggctgtg ttcatgactg tcatttctgc tcagtgcgcc gtttctatgg
     2581 ggatcgggtg cgtcatcgtc cgattgccga ggtcgtggcg gagttgcgaa cgcttgaccg
     2641 atcattcatc ttttttgttg atgacaacat tgctgccgac cccgaacatg cgatgaatct
     2701 gtttcaggcg ttgcgcccac tgggactccg gtggatcagc caggccagct tggcgaccgc
     2761 gcgacggccg gggtttctcg atgccatggc agaatcgggt tgtttcgcgg tgattctggg
     2821 gctcgagtcg ctgtcgccaa ccaatctgca gcgcatgaac aaggactgga ccctgaagct
     2881 cggtacgctg gaatcgctgc tggttgaata tcggcgacgg ggcattctgg tgtacggcac
     2941 gttcgtcttc ggatacgatg acgacacacc tgccgtgatc gaagataccg ttgatttcac
     3001 cattgctcag aggctgttca tggcgaactt caacattctg caaccgtttc ccggtacccg
     3061 ggtctacgag gaactggctg ccgatcgacg tctgttatac gagcgctggt ggctcgatgc
     3121 cgagtatcgc tgggagcgac cggcgtttcg cccgcgtcgc atgaccgtcg atgaactggc
     3181 cgcagccgtg gcgaatgccc gacaacgctt cggcagcgtg ggcaatctgc tgcggcgagc
     3241 gtgcgaccag tgggccaatg ttgcagatcc gtttcgggcg gcagttttcc tggcgtcaaa
     3301 tgtggtctcg cgtttggata tcaagcggaa aaccggcctg cgcctgggat ttcctgaacc
     3361 agaggcgatg gaggggactc gatgagggta acgttcatca aacccaacat gggcctcagt
     3421 aacgggcgcc cctattacga tcgaggtcgc atggagccgt tgaccttcgc ggttctggcg
     3481 ggccagttgc ccggcgacgt ggaatgccgg ttgtacgatg accgttttga acgcattccg
     3541 taccatgagc cgaccgacct cgtctgcctc aatgtggaaa tctacacggc gcgccgggcc
     3601 tacgagattg ctgatcgcta cagagcccgc ggggtgaagg tcgtgcttgg cgggtaccac
     3661 gtgaccatga ttcctgacga ggcggcgctt catgccgacg ccatcatgat cggtgatgcc
     3721 gaggggcagt tcgctcgtct cgtggccgac gcggccgccg ggcgcttgca ggctgtgtac
     3781 cgcggcgagt tcccaccgtc tctgaccggg ctgcgtaccc gttgggagat cttcaacggc
     3841 aaaagctatc tgcctctccg gctgacgcaa ttcagtcgtg gctgtgtcaa tcactgtcgt
     3901 ttctgcgcga ccggcacgat ctatcgccgc gtgcactgta cgcgacctcc ggccgaggtg
     3961 atcgacgaac tggcgcagca gccgcagccc tttgtattct tcgtcgatga caacatcatc
     4021 gggaacgtcg acgccgcgaa ggaactgttt cgtcggttga ttccgctgcg tcgccgctgg
     4081 atcggacaag ccagtctcac gttcaccgaa gatcccgaac tgatggatct gatgaaacgc
     4141 agcggttgcg cgggtctcgt ggtcggcttc gagtcggtca cgcccgaaaa catccggcag
     4201 atggacaaga actgcaacat gaatctcgac agctatgccc cggtgatcga gaagattcgt
     4261 cgggttggct ttcagctctg ggcagcattt ctcatcggat acgactggga cgattggacg
     4321 acgatccaca aaacggtaca atgggcgatc gatcagaggt tctgctttgc ggcgttcaac
     4381 attctgagcc cgtatccgag cacgccactc tacgaggaaa tgcagacgca ggggcgtctg
     4441 ttgtatgaaa agtggtggct cgacgaccgg tatcatttcg gcgactgtgt cttcaaaccg
     4501 gcccgcatga cccccgatga gttgaccgaa gcctgtttct gggcgcgtcg ccgcttcaac
     4561 catccgctga gcatcatcaa gcgggcgttc gatttccaga cgaacatgaa agacctctgg
     4621 agcctggcga cctacgtggc ctacaatccg ctgttccggc gcgaactgtt caagaaacac
     4681 ggcatggtgc tcgggtacca aaagcgctga gtctttttcg accgaatcgg atcgaaaaac
     4741 tctctgacag aagagagaca aatgtggtag aatgccggtc accctggaac agggctcttc
     4801 cagaacgcgt ctgaatttgc ccgggagacg tgtgtgtagg cggtctcgtg gccgtctgag
     4861 cggagcggtt ttctctcctc tgcctggtgg tgccgttcct gcgtcgg
//
LOCUS       DAORHD010000171         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18104_length_4898_cov_4.141235, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000171 DAORHD010000000
VERSION     DAORHD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18104_length_4898_cov_4.141235"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKO06_04980"
     CDS             <1..463
                     /locus_tag="PKO06_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV69032.1"
                     /translation="SHGLSPFSPLMIATFATLFGGLGFISLGLLSIFSNIIPSGLTNF
                     VSVAVSGSLALVLASYFSFFLVRIFVKTETASNIATSRLIGREAEVALDLEPGKIGEI
                     VYLHGGSRQNNMAKLADGCQPIRKGQMVEIVSIQENIMVVKPWENPGEARS"
     gene            568..2223
                     /locus_tag="PKO06_04985"
     CDS             568..2223
                     /locus_tag="PKO06_04985"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV69033.1"
                     /translation="MDPTTLITLGSLAILVFTVLIFILWSSRVQTVGPNEALIISGRP
                     NVIVDSQGNKHEVGFRIVKGGRSFVWPIIEKCERLSLELMTLEIQTPEVVTKQGVPIM
                     VDGVAQIKVKGEDFAIITAAEQFLGKSRSEIMAIAHQTLEGHLRAILGTMDVEELISS
                     RDAFASQVQAVSAPDLSRMGLIILSFTIKDIRDNQKYLESLGKRAIAERQRDAAIGQA
                     NASSEADISRAQAQSKAVEESSKFQKQAQVKKLANDAEIAQAARDFKMKNAEYEASVN
                     QKKAEADSAYNLQKYKQDQLIKAEEMQVSLIAKQKEIEIQKQEIERKKYELEALVEQP
                     AEAEKKKVETLASAEMFKLKTTAEGDSESTRLKGMARADVERAEGNAMADVQKAKGLA
                     EAEVEKAHGMAQAEVAKARGLADAEAVKARGLAEAESRRAIGLAEAEAMEKKAEAWRA
                     YNEAAISQLFIDKLPEIVRAVAEPLARTERIVMISSGNDAGVGASKLTKEIIDVVTQV
                     PPALEAVTGIDLGTMIGKIKGGSSDDTPKPVKPTPQPPQTPKK"
     gene            2371..>4898
                     /locus_tag="PKO06_04990"
     CDS             2371..>4898
                     /locus_tag="PKO06_04990"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNV69034.1"
                     /translation="MKRLLIAITILTLTAPMLAAQTTAAQQQLLLQGLSKSSLTGAGA
                     NSGSRISPTTTKSLEEAALFDKRLEMLTREQSGTPGTPEQEKANLESRLRELDNKVQY
                     LEQALRRKIIDEMVLEKLEKLQGEESKPKQRFDERLEEEKARLTSAPGFTEAELDRRV
                     KEFIGEYETEQEANKPFGSVFFERSADDIAQLENVPVPSTYILGPGDSLKIIVWSEMG
                     DETVYDVTVNPEGQVYIPIIGLFGVQGQTVGQFEEVVLGKLAEKFKHFKGQVTLSKIR
                     TLQIFVVGEVQKPGSMIVSALSTALHALVRAGGPSDKGTMRNIKVWRQNQVVAEIDLY
                     KYFMHGDKSQDILLESGDTLFVGPVGSRVFVKGQVIRPATYELAGENTLSEVLTMAGG
                     VAPGAYTRRVKIFRWQGNERRQIMDSEFASAKPEAFVVQNGDEVFVDKAIEDFGNEVT
                     IEGPVQKPGSYAVTEGTTIRELIKRAGGVIPEKIAKNAGQIIRKLAGGKEQLLAFKLE
                     AAVNGDAKHNLTVQPLDKVVLFSFDEVEPNVRKVVIAGAVRRPGEYIWREGMTVRDLV
                     LRAQGLTTDAATDAEIARMSGKENTQLQNISMEKIFENPADVNNVVLQPLDRVNILAR
                     GGEIIEAEVVYLKGEVVRPGPYAIKHRGETLTDLIKRAGGLTKRAFAEGTIFTRQTAN
                     LVAEQQLQIAEEVQGDLYKMASNDLKADLMRAGARFSEVTSDKTDAQVLGATSTAGLL
                     GGIGQGDFAEAEMRVQQAQQAQTKKESSSYSTFDSTGRTLMNKAARIPIRLDLAIRKP
                     QGDEDLELMDGDEIVIPPVPTTVSVVGAVVNPTNILFKDGKTVR"
BASE COUNT         1194 a         1358 c         1427 g          919 t
ORIGIN      
        1 ctcgcatggg ttgtcgccgt tcagtccgct gatgatcgca acatttgcca ccctctttgg
       61 cgggctgggg ttcatcagtc ttggcctgct cagcatcttc agcaacatca ttccgtccgg
      121 cctgaccaac ttcgtcagcg tcgccgtgtc aggcagtctg gccctggtgc tggccagcta
      181 cttctcgttc tttctggtgc gcatcttcgt caaaacggag actgcctcaa acattgcaac
      241 ctcgcgtctc atcggacgag aagcggaggt cgcgctcgac ctcgaacccg gaaagattgg
      301 cgagatcgtc tatttgcatg gtggcagccg ccagaacaac atggccaagc tcgccgacgg
      361 ctgtcagcca attcgcaaag gccaaatggt cgaaatcgtc tcgattcagg agaacatcat
      421 ggtggtaaaa ccctgggaaa acccgggaga ggctcgctcc tgaacaatgg ctacagttcg
      481 tgagacccgt ctgcagagtt tgattgactc tctggggttt ctcacttaga atttcacgtc
      541 tcaagtctgg ctggaggagg agagtacatg gatcccacga ccttgatcac cctcggttcc
      601 ctcgcgattc tggtttttac ggtcttgatc ttcattttgt ggtcgagccg agtacaaacc
      661 gtcggtccaa acgaagctct gatcatctcg gggcgcccca acgtcattgt tgactcgcag
      721 ggcaacaagc acgaagtcgg attccgcatt gtcaagggcg gccggtcatt tgtctggccg
      781 atcattgaaa agtgcgaacg actctcgctc gaattgatga ccctcgaaat tcagactccg
      841 gaagtcgtga ccaaacaggg cgtgccgatc atggtcgacg gcgtcgccca gatcaaggtg
      901 aagggcgaag atttcgccat catcaccgct gccgagcagt ttctcggaaa gagtcgctcg
      961 gagatcatgg ccattgcgca ccagacgctc gaaggtcacc tgcgtgccat tctcggcacg
     1021 atggatgtcg aagaactgat cagcagccgc gatgcgtttg cctcgcaggt gcaggccgtg
     1081 agcgctccag acttgagccg catgggtcta atcattcttt ccttcaccat caaagatatt
     1141 cgcgacaacc agaagtatct ggaatcactc ggcaaacgcg ccatcgccga gcgtcagcgc
     1201 gacgccgcga tcggtcaggc gaatgcctcg agcgaagccg atatcagccg ggcccaggcg
     1261 cagagcaagg cggtggaaga gtcgagcaag ttccagaagc aggcgcaggt caagaagctg
     1321 gccaacgacg ccgaaatcgc ccaggcagcg cgcgacttca agatgaagaa cgcggaatac
     1381 gaggcgtcgg tcaaccagaa gaaggctgag gcggacagtg cgtataatct gcagaaatac
     1441 aagcaggatc agctcatcaa ggctgaagaa atgcaggttt ccttgattgc caagcaaaaa
     1501 gaaattgaga ttcagaagca ggaaatcgag cgcaagaagt acgaacttga agccctggtc
     1561 gagcaacccg ccgaagcgga aaagaagaag gttgaaacgc tggcttccgc tgagatgttc
     1621 aaactgaaga cgaccgccga aggcgattca gaatcgaccc gcctgaaagg tatggctcgc
     1681 gccgatgttg agcgcgctga aggaaacgcc atggctgacg tgcagaaggc caagggtctt
     1741 gccgaagccg aagtcgagaa agcgcatggt atggctcagg ccgaagtcgc caaagctcgc
     1801 ggtctggccg acgctgaagc ggtaaaggct cgcggtctgg ccgaagccga aagtcggcgc
     1861 gctatcggtc tcgccgaagc cgaagccatg gagaagaaag ccgaagcgtg gcgcgcgtat
     1921 aacgaagccg ccatcagcca gttgttcatc gacaagctgc ccgagatcgt ccgagctgtg
     1981 gccgagcctc tggcgcgcac ggagcgcatt gtcatgatca gcagtggcaa cgatgcggga
     2041 gtcggtgcca gcaaactgac caaggaaatc atcgacgtgg tgactcaggt tcctccggcc
     2101 ctcgaagcag taaccggaat cgatctgggc acgatgatcg gcaagatcaa gggtggcagt
     2161 tctgacgata cgcccaaacc ggtcaagccc accccgcagc cgccgcaaac cccaaagaag
     2221 tagtcctcgt ctccatgcct tgaacgcagc accgtcaatt ctggaacaac atcgctcaga
     2281 atcgcatgaa tgttcgaaca aacagcgatg tcacccggac aatcgacggt gctgctttat
     2341 ttctgatctg acccggaagg aactcgccac atgaaacgtc ttctcatcgc catcaccata
     2401 ctcacgctca cggcccccat gctcgcagcg caaacgaccg ccgcacagca acaattgttg
     2461 cttcaggggc tcagcaagtc gtcgctcacc ggcgcgggcg caaattccgg cagtcggatc
     2521 agtccgacga cgaccaaatc gctcgaagaa gctgcgttgt tcgataaacg cctcgaaatg
     2581 ctgacgagag agcaaagcgg aacaccggga acaccggaac aggaaaaggc gaatctcgaa
     2641 agccgcctac gcgagctcga caacaaggtt cagtatcttg aacaagcgct gcgccgcaag
     2701 atcatcgacg agatggtgct cgagaagctg gaaaaactgc agggcgagga aagcaaaccc
     2761 aaacagcgat ttgacgagcg actcgaggag gaaaaagctc gcctgacctc tgcgccaggc
     2821 ttcacggagg cggaactcga ccgccgcgtc aaggagttca tcggggaata cgaaactgaa
     2881 caggaagcaa acaagccttt cggcagcgtt ttcttcgagc gttccgccga tgatatcgcg
     2941 cagttggaaa atgtccccgt cccctccacg tatattctgg gacccggaga ttctcttaag
     3001 attatcgtct ggtccgaaat gggtgacgag accgtctatg acgtcaccgt caatccggag
     3061 ggtcaggttt acattcccat tatcgggctg ttcggcgtgc aagggcagac ggttggtcag
     3121 tttgaagaag tcgttctcgg caagctggca gagaagttca aacacttcaa ggggcaggtg
     3181 acgctttcga agattcgaac cctgcagatt ttcgtcgttg gcgaagtgca gaagccaggc
     3241 tcgatgatcg tttccgcgct ctcgaccgcg ttgcacgctc tggtgcgcgc cggcgggcct
     3301 tctgacaaag gcaccatgcg caacatcaaa gtttggcgcc agaaccaggt tgtcgccgaa
     3361 atcgacttgt acaagtattt catgcacggc gacaagagtc aggatattct gctcgaatca
     3421 ggcgacacgc tgttcgtcgg tccggttgga tcgcgcgtct tcgtgaaggg gcaggtcatt
     3481 cgtccggcca cttacgaact cgctggagag aacacgctaa gcgaggtact gaccatggcg
     3541 ggcggagtcg ctcccggggc ttacacccga cgggtcaaaa tcttccgctg gcaaggaaac
     3601 gagcgtcgtc agatcatgga ttccgagttt gccagtgcga aaccggaagc gtttgtcgtg
     3661 caaaacggcg acgaggtctt tgtcgacaag gccatcgagg atttcggcaa cgaagtgacc
     3721 atcgaaggtc cggttcagaa acccggctcg tatgccgtga ccgaaggaac cacgatccgg
     3781 gagcttatca aacgggccgg tggcgtcatt cccgagaaga tcgcgaaaaa cgccgggcag
     3841 attattcgca agctcgccgg cggcaaggaa cagctgttgg ccttcaaact cgaagccgcg
     3901 gtcaacggtg atgccaagca taacctgacg gtccagccac tggacaaagt cgtgctgttt
     3961 tcgttcgacg aggtagagcc caatgttcgc aaagtggtca ttgccggcgc ggttcgacgc
     4021 cccggcgagt atatctggcg agagggaatg accgttcgcg acctggtatt gcgcgcgcag
     4081 ggactgacaa cggatgccgc aacagacgca gagatcgccc ggatgtcggg gaaagagaac
     4141 acgcagctgc agaacatcag catggaaaaa atctttgaga acccggcgga cgtgaacaac
     4201 gtggtcttac agcccctcga ccgggtcaac atccttgctc ggggtggcga gatcattgag
     4261 gccgaagttg tctacctgaa gggtgaagtc gtgcgacctg gcccgtatgc catcaagcat
     4321 cgcggcgaaa ccctgacgga tctgatcaag cgagctggtg gactgaccaa gcgcgctttc
     4381 gccgaaggca ccatcttcac gcgccagacc gcgaatcttg tcgccgagca gcagcttcag
     4441 attgccgaag aagttcaggg cgacttgtac aaaatggctt cgaacgatct caaggcggac
     4501 ctcatgcgcg cgggcgctcg cttttctgaa gtcacgtctg acaagaccga tgcgcaggtt
     4561 ctcggagcaa catcgacggc aggcctgctc ggcgggattg ggcaaggcga tttcgccgaa
     4621 gccgaaatgc gagtgcagca ggcacaacag gcacagacga agaaagagag ttcttcatac
     4681 tcaacttttg acagtaccgg ccgcaccctg atgaacaaag cggcccgcat tccgatccgt
     4741 cttgatctgg caatacgaaa gccccagggt gacgaagatc tcgagttgat ggatggcgat
     4801 gagattgtca ttccgccggt accaaccacc gtgtctgtcg tgggcgccgt ggtcaatccg
     4861 acgaacattc tgttcaagga tggtaagacg gtacggca
//
LOCUS       DAORHD010000172         4895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18116_length_4895_cov_4.303719, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000172 DAORHD010000000
VERSION     DAORHD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18116_length_4895_cov_4.303719"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2568
                     /locus_tag="PKO06_04995"
     CDS             <1..2568
                     /locus_tag="PKO06_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69035.1"
                     /translation="EIHVNLLIDRLKALQISPDQIVSRIRQENLNLPAGIVEAGNYEF
                     TIRTPGEFYKVEQLKAIVVKEDRVNQIRLGDVAEIDDRWERARNIVRVNGKPGVRVSI
                     SKQSGKNTVQVADAVLAEIEQINRDLPQLQLTPIVDTSKYIRNAIENVSSSALQGGAL
                     AILVLMFFLADLRSTIIIGVSIPISIIATFALMFYYGFTLNIMSLGGVALGIGMLVDN
                     SIVVLENIFRRRELGQPPEEAAIDGSVEVTAPIIASTLTTLVIFLPLLFVEGMTGLMF
                     KQLSYVVSFSLVCSLFVSLTLVPMLCARLLKQVQVKNGQVTGNGITRLLKSIVGFFEN
                     RYLEILKFTLNFRFLVTGLVLLLLATTIYLARTIPAEFMPTADEGDVRVTVELEPGSR
                     LSLTDDKVRQVERIVQENVPERLTMVTTVGGGGGMGGGGSSGVNRGEIRIPLVDRTKR
                     KRNSARIAGELRGLLSQIPGIRIRTREGQGLFMFRMGAGNADKIQVQVRGHKLEQADH
                     LARQVEKLLKKIPGISDIQLSRESGVPERLISIDRQRASDQRLTVTRVAQFLETLLSG
                     SGAGNFRESGDEYRILVKALNAERVLLEDIMDLTILNAFGQPVVLRNLASIVSREGPL
                     LIERADQERVVNVSADPGERSVGEVLNEVQDALKTIPLPNGFALALAGDYEERQKAFR
                     ELLISFCLALILVFMVMAIQYESLLDPIIVMVTVPLSTIGVIVALVATGTTFNVQAFI
                     GCIMLGGIVVNNSILLVDHANDLYRTQKRPLLESLLEAGRDRFRPVLMTAATTILGLV
                     PMALGLGDGGEVQAPMARVVMGGLICSTGISLVYIPVVYAFFAGWSQQEQVQHEQQK"
     gene            2642..3313
                     /locus_tag="PKO06_05000"
     CDS             2642..3313
                     /locus_tag="PKO06_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69036.1"
                     /translation="MISFILVSQALSAQYLPFLNWDRISFPMIEPEFWGAASETTGDI
                     ASGSQPGHFWRLSLDEAIATAIENNHEFKIRRIEPEQQAVERERRRSEFDTTFGATVS
                     NSDRLGRQIQQSGALGDNVANRTDVGVSWAKRFPSGTEASLEVTTGRNRSARAPNLFS
                     TRVGLELTRPLRQGAGRTVNLVSLQSAELDEQISKYELEAYLLGLVAQIEKGYWDFFL
                     ALKER"
     gene            3344..4198
                     /locus_tag="PKO06_05005"
     CDS             3344..4198
                     /locus_tag="PKO06_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69037.1"
                     /translation="MQRDETSKRIELGSIPESELAAAEAEVALREEAYINAGSQIETT
                     RISLLRMINPNSDEFWKFHLELSDEPADLDFPATSPQDLIEIALEKRPELRQAQLDLE
                     KGKLELVRTKNGLLPRLDFFTTLGKSGYAQTFEKSADKFGLKGYDLQAGVLYELALGK
                     REARMQVKKSELSIKRSQEAIDNLKQLVQEDVLKAFLEAKRAALQIRATEKTVAKQQE
                     KVRVETIKFGLGKTTAFQVAQAQRDVAASQIAALKSRVGYLVALTDLYRYAGILCERR
                     QLKIAVAN"
     gene            4260..>4895
                     /locus_tag="PKO06_05010"
     CDS             4260..>4895
                     /locus_tag="PKO06_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNV69038.1"
                     /translation="MINSKRGETQPALPFAHPDLAFLIALTEATTDPILEVYRQDFAV
                     DLKSGQEPVTEADRRANDLIVRLVHERYPDDALLTEEAGFDAGHDGHAHRVWFVDPID
                     GTKEFIRKNGEFAVQIALVCDRRLELGAIFRPTTGDLYVAARGKGCHVRRRENGNDTS
                     WQRLKCVPSHAGSLIVAMSRSHPSSLAQRAGERVGLTGEFRHGSVGLKLMAM"
BASE COUNT         1048 a         1274 c         1483 g         1090 t
ORIGIN      
        1 gaaatccatg tgaatctgct catcgatcgt ctgaaggcgc tgcagatttc tccagatcaa
       61 atcgtcagtc gcatcagaca agagaacttg aatctgccgg cgggtatcgt cgaagcaggc
      121 aactacgagt tcaccattcg tactcctggg gagttttata aggtcgagca actcaaagcc
      181 atcgtggtga aagaagaccg ggtgaatcag attcgtcttg gtgacgtggc cgagatcgac
      241 gatcgctggg agcgggcacg caacatcgtg cgggtcaacg gcaaaccggg ggttcgcgtc
      301 agcatcagca aacagtcagg caagaacacc gttcaagtgg ccgatgcggt gctggccgaa
      361 atcgagcaga tcaaccgcga tctgcctcag ctccagttga cgccgattgt cgatacatcg
      421 aagtacattc gcaatgcgat cgagaacgtc agttcatctg ccttacaggg tggcgctctg
      481 gcgattctgg tgttgatgtt ctttctcgct gacctgcgca gcaccatcat tatcggtgtt
      541 tcgattccga tttcgatcat tgcgacgttt gcgttgatgt tctactacgg cttcaccctg
      601 aacatcatgt ctctgggtgg tgtcgcgctc ggcatcggca tgctggtcga caactcgatc
      661 gtggttctcg aaaacatttt tcgtcgtcgc gaactgggcc agccacccga agaggcggcg
      721 attgacggca gtgttgaggt cacggcaccg atcattgcca gtacgttgac gacgctggtc
      781 atctttctcc cgctgttgtt tgtcgaaggc atgaccggct tgatgttcaa gcagctttcg
      841 tatgtggtca gcttttcgct ggtctgttca ttattcgtgt ctctgacgct ggtgcccatg
      901 ttgtgcgcca gacttctcaa gcaggtgcag gtgaagaacg gacaggtgac cggcaatggc
      961 atcacccgac tgctgaaatc gatcgtgggt ttctttgaga atcggtatct tgagatcttg
     1021 aagttcacgt tgaattttcg gtttctcgtg acaggacttg tacttttgct gcttgcgacc
     1081 acgatctacc tggctcgcac tattccggcc gagttcatgc cgacagcgga cgaaggcgat
     1141 gttcgggtga cggtcgagct tgaaccgggc tcgcggctca gtctgacgga tgacaaagtt
     1201 cgtcaggtgg agcggatcgt tcaggaaaat gtgcctgagc gactgacgat ggtcacgacg
     1261 gtgggcggcg gtggcggcat gggtggcggc ggaagttcag gggtgaatcg tggagagatt
     1321 cgcattcctc tggtcgaccg cacgaagcga aagcgaaaca gcgcgcggat cgcgggtgaa
     1381 ttgcgcggct tactgtcgca gattccagga attcggattc gtacccgcga ggggcaggga
     1441 ttgttcatgt ttcgcatggg cgcgggaaat gctgacaaga tccaggtaca ggtgcgcgga
     1501 cacaagctcg agcaggccga ccacctggcc agacaggtag aaaaactcct gaaaaagatt
     1561 cccggcatct cggatatcca gctcagtcgc gagtccgggg ttccggaacg cttgatttcg
     1621 atagatcgac agcgggcttc tgatcagcgc ctgacggtga cacgagtggc acagtttctc
     1681 gaaactctcc tttccgggag cggcgcggga aactttcgcg aaagtggcga cgagtatcgt
     1741 attctcgtca aagccctgaa tgccgagcgc gtcttgctcg aagacatcat ggatctgacg
     1801 attctgaatg ccttcggcca gcctgtcgta ttgaggaatc tggccagcat cgtctcgcgg
     1861 gagggaccat tgctgatcga gcgtgcggat caggaacgag tggtgaacgt gtccgctgat
     1921 ccgggtgagc gctcggttgg cgaggtcttg aacgaagtgc aggacgcctt gaagacgatt
     1981 cccctgccga acggctttgc tctggcgctg gcgggagact acgaagagcg gcagaaagcc
     2041 ttcagggaac tgttgatcag tttctgtctg gcgctcatac tcgttttcat ggtcatggca
     2101 attcagtatg aatcgttgct ggacccgatc atcgtcatgg tgacggtgcc gctttccacc
     2161 atcggagtca ttgtcgctct ggtcgccacg ggcacgacct tcaacgtcca ggcgttcatt
     2221 ggttgcatca tgctgggggg catcgtggtg aacaattcga ttctgctggt cgatcacgcc
     2281 aacgatctgt atcgaactca gaaacgacct cttctcgaat cgcttcttga agcgggacga
     2341 gatcgttttc gaccagtact gatgacagcg gctacgacaa ttctcgggct ggtgcccatg
     2401 gctctcggtc tgggagacgg cggcgaagtg caggccccga tggctcgcgt tgtcatgggt
     2461 ggtctaatct gctcgaccgg tatttcgctg gtctatatcc cggtggtcta tgcattcttc
     2521 gccggttgga gccaacagga acaggtgcaa catgaacaac agaagtgaac gagacaggtc
     2581 ctcaggactg gctttctccc tgcgaggtac aggacgacga aacgggaggc tggcatgggt
     2641 catgatctcc tttattcttg tgtcacaggc gctttctgca cagtatctgc catttctgaa
     2701 ctgggacaga atctcttttc ccatgatcga gcctgagttc tggggagcag catcagagac
     2761 cacaggcgac attgcttctg gcagtcagcc cgggcatttc tggcgtctga gcctcgatga
     2821 agcaattgca accgcgattg agaacaacca tgagttcaag attcggcgca ttgaaccgga
     2881 acaacaggcg gtcgagcgag aacgtcgccg atctgagttc gacacgacct tcggggcaac
     2941 tgtttcgaac tcggaccgac ttggcaggca gattcagcag agtggcgctc tgggtgacaa
     3001 cgtggcgaat cgtaccgatg tcggagtttc ctgggcgaag cgttttcctt cgggtaccga
     3061 ggcttctctc gaagtcacca cggggcgcaa ccgttcggcg cgcgcgccga atctgttcag
     3121 tacgcgcgtg ggcctcgaac tgacccgacc acttcgacag ggagcgggaa ggacggtcaa
     3181 tctcgtgtcg ctgcagtcgg cggaattgga cgaacagatc tcgaagtatg aacttgaggc
     3241 ctacctgctt ggtctggtag ctcagatcga gaaaggatac tgggactttt ttcttgccct
     3301 gaaagaacgg tagatcgtca agacctctct cgaactggcg cgtatgcaac gtgacgagac
     3361 atccaagcgc atcgaactcg gcagcattcc tgagtccgag ttggcggcgg ctgaagccga
     3421 ggtggcactt cgcgaagagg catatatcaa tgcgggcagt cagatcgaaa ccacgcgtat
     3481 ttcgctgttg cgcatgatca acccgaattc ggacgagttc tggaagtttc atctcgagct
     3541 ttctgatgaa ccggcggacc tggactttcc ggccacctca ccacaggatc tgatcgagat
     3601 cgcgctcgag aaacgccctg agctgcgaca agcccaattg gatctggaaa aaggaaagct
     3661 cgagcttgta cgaacgaaaa atgggttgtt gccacgactt gacttcttca cgacgctcgg
     3721 caagtctggg tatgcgcaga ctttcgagaa gagtgccgac aagttcggtc tgaaaggcta
     3781 cgatctgcag gcgggtgtgt tgtatgaact ggccctggga aaacgcgaag cccgcatgca
     3841 ggtgaagaag tctgagttgt ccattaagcg ctctcaggaa gccattgata acctgaagca
     3901 acttgtacag gaagatgtat tgaaagcgtt tctcgaagcc aagcgtgcag ctctgcagat
     3961 tcgcgcgacc gagaaaacgg tggcgaaaca gcaggagaag gtacgcgtcg aaacgatcaa
     4021 gttcgggctc ggcaaaacca cggcctttca ggtggcgcag gctcagcgcg atgtcgcggc
     4081 cagtcagatt gccgcgttga aatcacgagt cggatatctg gttgccctca ccgatctcta
     4141 tcgttatgct ggaattctct gcgagcgccg ccagctcaag attgccgtcg ccaactgaat
     4201 gacgtggctg cacccaccag tgatgcgtcg gcgtccatga accgaaccgg agcccttcca
     4261 tgatcaactc gaaacggggt gagacacaac ccgcgctgcc ttttgcgcac ccggatctgg
     4321 cttttttgat cgcgctgacc gaggcaacga cggatccgat tctcgaggtc tatcgtcagg
     4381 attttgcggt ggatctcaag tctggccagg aacccgtcac cgaggctgat cgccgagcca
     4441 acgatctgat tgtgcgcctg gtgcacgaac gctacccgga cgatgccctg ctcacggaag
     4501 aagccgggtt cgatgccggg catgatgggc acgcacatcg tgtctggttc gtggacccga
     4561 tcgacggcac caaggaattc attcgcaaga atggggagtt cgcggtgcag atcgccctgg
     4621 tctgtgatcg acgtctggaa ctgggggcca tttttcgccc gacgaccggc gatctttatg
     4681 tcgcagcgcg tggaaaggga tgccatgtgc gtcgaaggga aaatgggaac gacacctcat
     4741 ggcagcggct caaatgtgtg ccgtctcacg cggggtcgct cattgtggcg atgagccggt
     4801 cgcatccgag ttctctggcc caacgagccg gcgagagggt gggactgacc ggagagttcc
     4861 ggcacgggag cgtggggctc aagctcatgg ccatg
//
LOCUS       DAORHD010000173         4884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18171_length_4884_cov_4.207289, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000173 DAORHD010000000
VERSION     DAORHD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18171_length_4884_cov_4.207289"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKO06_05015"
     CDS             complement(<1..1128)
                     /locus_tag="PKO06_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69039.1"
                     /translation="MRFSSVVIGVLFLAAMFCTCANALTKEEALKLYKKYKADKEKNA
                     SGESGNSSTENSGEEGENAEGDKPEGENESGESSESGENSESGESSSSGESTGSTGQI
                     SISSGDSGSSEVSTTTSQSSSGSSGSSGTPTSGTAEPATSDDMQTKTLTPEPDDPTDK
                     RYCEGHMKLARRHFKNNDFVRAMEEVTLVIERYSLHAEARFLKAVLFAKQKKYIDAWR
                     NIEVAQKTDAENPRIKDFVTRLEKAFPKPAELPEESTVATRPEPKFGSALLVNALEAS
                     VADKALAGKIEKIACADPTDAGGHLSVVITFTATDKLDATTLQTKLKDVGKLETSDPK
                     IASEGKQVTLTATISGLPFQNPDLKPIDKLSEFLRETSEECD"
     gene            1287..2198
                     /gene="speB"
                     /locus_tag="PKO06_05020"
     CDS             1287..2198
                     /gene="speB"
                     /locus_tag="PKO06_05020"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HNV69040.1"
                     /translation="MGLDAVHFRGGYNFLGLPEEDTRYENAHAVILPVPYDSTTSYKA
                     GTREGPLALILASRNVELYDLELECEPLISGIHTLPELEPDMRGPEVTVRNIQKAVEA
                     LVKDGKFPVVLGGEHSITTAPVRVLAKKYGADFSVLQLDAHADLRESFENTPFNHASV
                     MRRVRELCPVTQVGIRNISAGEMEYVRQSRHKTIFWAHDLQGPAKTWIPSVVKTLKKK
                     VYITIDLDAFDPAVCPGVGTPEPGGLTWYPTLELLQTVIRTHDVVGIDIMELCPIAGS
                     IVSDFFAAKLLFKLLAEVFAKNHWIGE"
     gene            2204..3028
                     /locus_tag="PKO06_05025"
     CDS             2204..3028
                     /locus_tag="PKO06_05025"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HNV69041.1"
                     /translation="MSLLQPGNIVTAVLAAVLGLLAGPRVTWAIRAWPGHEAFDSRYL
                     KCSTCAGGLRRGCYNAGVAQDYVFATVTMLLAFLSVWYYGVGSKAIFSWIFATACLII
                     TVVDYRYLIIPDKLSINGFYIGLLYHGVIALWISYGGGTPPEYYLPFRDSLFGFLLGG
                     GFLWALRAGAWIILKKEGMGGGDIKLLATMGAWLGYQPVVGIIIVASMLGTIGGIGQI
                     LVNRLIYGKKYRPFSHMIPFGPYLCLGFLLIFYAGMEPLWWLMEQYQRWLEAHLMR"
     gene            complement(3209..4732)
                     /locus_tag="PKO06_05030"
     CDS             complement(3209..4732)
                     /locus_tag="PKO06_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-arginine deiminase family protein"
                     /protein_id="HNV69042.1"
                     /translation="MQSRCPARSVFLGLILIVALVTGTASGALKELKEKDIEALRIVS
                     QANLPHKILVASQIQANLKFLTSLIMTVAKIHEEDRLGPEDSFKVSIIPSMGEKITGQ
                     TPLRKAVPKKIFEKYVEYIPQETWSDPWLQDIGEVAMVKVKNDPKPQLLIIDSQRGQG
                     LAALPEFLAKLWNSYYLKNPTDRQPGGDSGGNIEATPDDILIIGSRSSKELRDLFARH
                     GYQNRMAVVDTDWLVVGHCDEYLSAVPTDRHPDGYGLVKANPRLALRLLADASRQELE
                     NVRPAEFRNRCLAIFNFLEEARQRANARQGETSDPLTFGEQLLIYLENVDPQTGMVSE
                     QARIRYGSVWNRAFQKNLGEREVDGESVERFIRANLALANLIDTNVELVRDTVNRVHA
                     RPSKTPIGVHSFPMLFEPYHQAPQYGKIRFVSSFPGVVNQLILRRHLLVPDPLITAFR
                     QQIRTTAKALGMKSHFIDDMYYHQLLGDVHCATNVFRLPNRYIIKPANLPAIWQPRQ"
BASE COUNT          931 a         1287 c         1476 g         1190 t
ORIGIN      
        1 atcgcactct tcactggttt cgcgcaggaa ctcggatagc ttgtcgatcg gcttgagatc
       61 ggggttctgg aacggcagcc ccgagatggt ggcggtcagg gtgacctgtt tgccttcgct
      121 ggcaatcttg ggatctgacg tttcgagttt gccgacgtcc ttgagtttgg tctgcaaagt
      181 ggttgcgtcc agtttatcgg tggccgtaaa cgtgatgaca acgctgagat gcccgcccgc
      241 atctgtcggg tcggcgcagg cgatcttttc aatcttgccg gcgagtgctt tgtcagccac
      301 gcttgcttcg agggcgttga cgagcagggc tgacccgaac ttgggctcag ggcgggttgc
      361 cactgtcgat tcttcgggca gttcagcagg cttgggaaac gctttttcca ggcgggtgac
      421 aaagtctttg atgcgcgggt tttcagcgtc ggttttctgc gcgacttcga tgttgcgcca
      481 ggcatcgata tactttttct gtttggcaaa aagaacggcc ttgagaaagc gtgcttctgc
      541 atgcaggctg tagcgctcga tgaccagcgt gacttcctcc atggctcgga caaaatcgtt
      601 attcttgaag tggcgccggg cgagtttcat gtgaccttcg caataccgtt tatcggttgg
      661 atcatccggt tcgggggtca gcgtctttgt ctgcatgtcg tcgctggttg cgggttcggc
      721 ggttccgctc gtgggtgtgc ctgaactgcc gctggacccg gatgaagatt gggacgtcgt
      781 ggtcgagacc tcggaagaac cactgtcacc ggagctgatg ctgatctggc cggtactgcc
      841 ggttgactca cccgatgatg aggattcgcc cgactcggag ttttctccgg actcagaaga
      901 ctcgccggat tcattttcac cttccggttt gtcaccttcg gcgttttcgc cttcttctcc
      961 tgaattctca gttgaagagt tgccgctttc tccagaggcg tttttttcct tgtcggcctt
     1021 gtactttttg tacaatttca acgcctcttc cttggtgagg gcattggcgc aggtgcagaa
     1081 catggctgcg agaaaaagaa ccccgatcac gactgacgag aatctcatcg ttcgtctcat
     1141 gtgtggtgcc tcacgtgctc gaagtggaac ggccggcgaa gaccctgtcg ctcgctgatc
     1201 atgctatcag aaatgcacaa cggttgccag cgctcccgga ttccactatg atggcgtctc
     1261 attcttcact ccccggaggc catgtcatgg gacttgatgc agtgcacttt cgcggcggtt
     1321 acaattttct cgggttgccg gaagaagaca cgcgatatga gaatgcgcac gccgtgatcc
     1381 tgccagttcc ctatgacagc acgacctcgt ataaagccgg aacccgcgaa gggccgctgg
     1441 ccctgattct ggcatcgcgc aacgtcgaac tgtacgatct ggaactggag tgcgagccgc
     1501 tcatttccgg aattcatacg ctgcccgagt tggaacccga catgcgtggc cccgaagtga
     1561 ccgtcaggaa cattcagaag gccgttgagg ccttggtgaa ggacgggaag ttcccggtcg
     1621 ttctcggtgg tgaacacagc attacgacag cccccgtgcg cgtactggcg aaaaaatacg
     1681 gggcagattt cagcgttcta cagctcgatg ctcacgccga cctgcgcgaa tcgttcgaga
     1741 acaccccctt caatcatgcc agcgtcatgc gccgcgtgcg tgaattgtgc ccggtgacgc
     1801 aggtgggcat tcgcaacatc tcggcaggcg agatggagta cgttcggcag agtcgccaca
     1861 aaacgatttt ctgggcacac gatctgcagg gaccggcgaa aacctggatt ccatcggtgg
     1921 tcaagacgct gaaaaagaag gtttacatca ccatcgatct cgatgccttc gacccggctg
     1981 tgtgtccggg ggttggcacg ccggaaccgg gcgggttgac ctggtatccg actctcgaac
     2041 tgctgcagac cgtcattcgc acgcatgacg ttgttggcat cgatatcatg gagctgtgcc
     2101 cgatcgccgg ttccatcgtc tcggacttct ttgccgcgaa actcctgttc aagctgctgg
     2161 cagaggtgtt tgcgaagaat cactggatcg gagaatgatg ctcgtgtctc tgctgcaacc
     2221 gggcaacatt gtgacggccg tcttggccgc cgttctgggg cttctggccg ggccgcgggt
     2281 tacctgggcg attcgtgcct ggcccggaca tgaagcgttc gactcgcggt atctgaagtg
     2341 ctcgacctgt gccggcggac ttcgtcgcgg gtgttataac gcgggtgtcg ctcaggacta
     2401 tgtttttgcg accgtgacga tgctgctggc ctttctgtcg gtctggtact acggtgtcgg
     2461 cagcaaggcg atcttttcgt ggatcttcgc caccgcctgt ctgatcatca cggtggtcga
     2521 ttatcgatat ctgattattc cggataagct ttcaatcaat ggtttttaca tcggcctctt
     2581 gtaccatggc gtgattgcac tctggatcag ttacggtggc ggaactcccc cggagtatta
     2641 cctgcccttt cgcgacagtc tgtttggctt tctcctgggc ggcggctttc tctgggcatt
     2701 gcgggccggc gcctggatta tcctgaaaaa ggaaggcatg ggcggcggtg acatcaaact
     2761 gctcgcgacc atgggtgcct ggctcgggta ccagccggtc gttggtatca ttatcgtggc
     2821 cagcatgttg ggaaccatcg gggggatcgg tcagattctg gtgaatcgtc tcatctacgg
     2881 gaaaaaatac cggccgtttt cgcacatgat cccctttggg ccctatcttt gccttggttt
     2941 cctgctgatt ttctatgccg gaatggagcc actctggtgg ttgatggaac agtatcagcg
     3001 ctggctcgaa gctcacctga tgcgctgaat gacgcgagcc tgggcaagag tccaggttgc
     3061 gaatgaagaa aacgcacttt ttaaagctct cattggcatt gtaatacgaa gagagcgttg
     3121 tgctcgtcgg tgacggttgt cagccgagta aaaacggccc gagtgatgaa gtcactcgag
     3181 ccgttctctt gtcggcacgc cgggtgtgtt actgtcgagg ctgccagatg gcgggcaggt
     3241 tggcgggttt gatgatatag cggtttggca gtcggaacac gtttgtggcg cagtggacgt
     3301 cacccagtag ctgatgatag tacatgtcat caataaagtg cgacttcatc ccaagggctt
     3361 tggcggtggt gcgaatctgc tgacgaaaag cggtgatgag cggatcggga acgagcaggt
     3421 ggcgtcgcag gatcagttga ttgaccaccc cggggaatga ggacacgaag cggatctttc
     3481 catactgcgg agcctgatga tagggttcga acaacattgg gaacgagtga acgccgatcg
     3541 gcgtctttga gggacgagca tgaacacgat tgacggtgtc ccgcaccagt tcaacgttgg
     3601 tgtcgatgag attggccaga gccagattgg cgcgaatgaa ccgctcgacg ctctcgccgt
     3661 cgacctcgcg ttcgcccagg ttcttttgaa aggcgcggtt ccagacgctt ccatagcgaa
     3721 tgcgggcctg ttctgaaacc atgccggtct gcgggtcgac gttctcgaga tagatcagca
     3781 gctgttcccc gaacgtcagc gggtccgagg tttctccctg acgtgcgttg gcgcgctgac
     3841 gagcctcttc gagaaagttg aagatggcca gacagcgatt gcgaaactcc gctggacgca
     3901 cgttttcgag ttcctggcgc gaggcgtcag ccagaagacg cagggccagt cgtggattgg
     3961 ctttgaccag accgtaaccg tccggatgtc gatcggtggg aacggcggaa agatactcat
     4021 cacagtggcc gaccacgagc caatccgtat cgacgacggc catccggttc tggtatccgt
     4081 gacgcgcaaa caagtctcgc agttcctttg agctgcgact accgatgatc aggatgtcat
     4141 cgggcgtggc ttcgatgttg ccgccgctgt cgccgccggg ttgtcgatcg gtggggtttt
     4201 tcaggtaata gctgttccac agtttggcca gaaactccgg cagagcggca agaccctgtc
     4261 cgcgctggga gtcgatgatg agcagttgcg gtttcgggtc gttcttcacc ttcaccatag
     4321 cgacttcgcc gatatcctgc agccacggat cagaccaggt ttcctgcgga atgtactcga
     4381 cgtacttctc gaagatcttt ttcggaacgg ctttgcgcaa aggagtctgt ccggtgatct
     4441 tttcgcccat cgaagggatg atgctgacct tgaagctgtc ttcggggccc agacggtctt
     4501 cttcatgaat cttggcgacc gtcatgatca gcgaagtgag aaacttcagg ttggcttgaa
     4561 tctggcttgc caccaggatc ttgtgtggca ggttcgcctg gctgacgata cgcagagcct
     4621 cgatgtcctt ttccttcagc tctttcagcg caccggatgc cgttccggtc accagggcca
     4681 caatcaggat cagaccaaga aatacggagc gagcgggaca gcgactctgc atgatatccc
     4741 tcctggtagg gcgaagtaat agcaacccga ctaggctaac acagagctcg atgggcgtgg
     4801 aaggattatt cccattttgt ggtagactgg cacggctatt cactgaacac ggtcggaggt
     4861 ctgtcatgtc ggtcgtcggc aggt
//
LOCUS       DAORHD010000174         4882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18185_length_4882_cov_2.854154, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000174 DAORHD010000000
VERSION     DAORHD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18185_length_4882_cov_2.854154"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PKO06_05035"
     CDS             complement(<1..646)
                     /locus_tag="PKO06_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV69043.1"
                     /translation="MLHRVMNRLIIVLLLSSWALVPVWSEQFPLRILHTNDWHGTFQP
                     LPSVWRDASAPRPIGGAAILGTVLSQRKADILRRGGRYLLVDAGDLFQGSMEVNLTKG
                     MAMIDLYNHLGYAVTALGNHDFDYGPQALRAAYARARFPVVCANLEGRDRFWRNSLRT
                     SIGPVKVGVIGVITPELPAVSVKKHLSGVRLRNIASTASAEAAALRRQGCDLVIL"
     gene            complement(697..2367)
                     /locus_tag="PKO06_05040"
     CDS             complement(697..2367)
                     /locus_tag="PKO06_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV69044.1"
                     /translation="MKRETGRVFSRWFLLMLLCTVGCGDAPKPEATKPLEVTPVQGGV
                     FRRAFASTGLSLDPREVADSFSHEVCRQMYDGLVEFDQEARVSPVIAESWQVSADRLT
                     YTFKLRRDVKFHQVCGENDASTLNAGRVCTAEDVDYTFHRLLDPAMKSQRGQEFLVIR
                     GATDYATGKASRIDGIKVLASDSLSMTLEKPFSPFLQLLALSNAFIVPADEARRDNPK
                     LGALPVGSGPFRYAGWKGETLILKANPDYFRGRPYLDTLEFPIIRDEKERFAAFKRGE
                     LLINDVPDPEYKNVKTDPSWAPHFQETSRWGTYYIGFNVGKPPFDNPKVRQAINYAID
                     RETIVNLILNGRARVAKGVLPPGILGYNPNLKGYSYDLPRAKKLLAEAGYPDGKGFPE
                     ISLQYNRDPAHVRSSEFILANLRDLGITCTLKEVEFTEHLRSVENGDVGFFRMGWTVD
                     YPDPDNFLFTLFHSSNFGAKGNFTRYANPRLDELLEKARFELDARDRIPIYHQAEQMV
                     VDDAPWVFVYHYTTHVIAQPQVRGLRITPLGLPFVLYRQLWLAPAKGG"
     gene            2602..3657
                     /locus_tag="PKO06_05045"
     CDS             2602..3657
                     /locus_tag="PKO06_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNV69045.1"
                     /translation="MARLIAVFLFLTILITSSPVWSAERSDRIPHRTVYAIRCLARDN
                     IWIGTEDGAARFDGRTWTIYRTADGLPHQVVTSIAEDPQDGTLWFGTFGGIARFRNER
                     FESFSTSNSGLINDVVYAVTVTPPHVWAATTNGLSRFHPQTREWKSFNDQNAPMHEVW
                     CYGATTEATTGETWFAIWGSGILRVKANGDFVSYTDPDEIFDVDTVRNDGPLHEIQVA
                     IDTHQNVVYSGAYFGLTRFDTAANKWNTWTIKDPDSGLPSDFINTVRLSPDGKICAIG
                     TDKGLCLFNHDRQSWVTYRRSAQGGGEYRIFEKGAWRTESAPQAPAGDFIWSIDFLDD
                     TIWVGTASGLYTASIKQ"
     gene            3748..>4882
                     /locus_tag="PKO06_05050"
     CDS             3748..>4882
                     /locus_tag="PKO06_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV69046.1"
                     /translation="MNAYHQWTDPFEYHGPAREVTEGEQNPPTVKVGVLCSLSGNGEE
                     YGQAMKQGADMATDEINRAGGLLGRPLELVYRDDRNDMGENGHQTVKMIFDDKVWAII
                     GSVHSGCTHVAARVTLKAETPQLTTVSTDPTVQMIGSPWMFRCLADDKAQGTAIANVV
                     FDRQKLLRVGLFQQKNRYGKMGGKTIGQIAEHRGKPLIFKNFFESGQTDFSAQVQVAK
                     NSNADGIIIWGLYQEAGLLVKALRDAGLKAQIFGADGMVSPGFIRIAGTSAENVIVTY
                     PFDDTNPAPATQKFIADFERRYGHRPDSFAAHAYDAAWMLIYGFAWAEYQEDSVASGL
                     TLARGLRKLSAPGVPAADQVPVRPSSWNEVKASFSDGAAVNLLG"
BASE COUNT         1069 a         1474 c         1388 g          951 t
ORIGIN      
        1 gcaaaatcac cagatcacat ccctgtcgac gcaacgcggc tgcttcggca ctggcagtcg
       61 aagcaatgtt ccgcaggcga accccggaca aatgcttttt gacggatacc gccggcagtt
      121 cgggggtaat cacgccgatg actccaacct tcaccggtcc aatgctggtg cgcagcgaat
      181 tgcgccagaa ccgatcgcgc ccttccagat tagcgcacac gacggggaac cgagcacgag
      241 cataagccgc ccgtaaggcc tgcggtccat agtcgaaatc gtggtttcca agagccgtga
      301 cggcatagcc caagtgattg tacaagtcga tcatggccat acccttggtc agattgacct
      361 ccatcgatcc ttgaaacaga tcgccggcat cgaccagcag gtaacgcccg ccgcgccgca
      421 gaatgtcggc cttccgctgc gacagcaccg tgccgagaat cgccgcaccc ccgatcggac
      481 ggggtgccga agcgtcacgc cagaccgaag gcagcggctg aaacgtgccg tgccagtcgt
      541 tggtgtgcag gatccgaagc ggaaactgtt cagaccagac cggtaccagc gcccaggagc
      601 tgagtagaag aacaatgatc agacgattca tgactcgatg cagcatatga ttcctcaaag
      661 gcaagtgtcc tgaagacatc aacgatcgca cagggcttac ccgcctttcg cgggagcaag
      721 ccacaactgc cgatacagaa cgaacggcag acccaggggg gtaatgcgca gtccacgcac
      781 ctgcggctgt gcgataacat gcgtggtgta gtgatagaca aacacccagg gggcatcatc
      841 gaccaccatc tgttccgcct gatggtagat cggaatgcgg tctcgggcat ccagttcgaa
      901 acgagctttc tcgagcagct cgtcaaggcg gggattggca tatcgcgtga agttcccctt
      961 ggccccgaag ttcgacgagt gaaagagcgt gaacagaaaa ttgtccgggt caggataatc
     1021 gacggtccag cccatgcgaa agaagcccac gtctccgttc tcgacggagc gcagatgctc
     1081 ggtgaactcg acctctttga gcgtgcaggt gatgccgaga tcacgaaggt tggccaggat
     1141 gaactcgctc gaccgcacat gagccggatc gcgattgtac tggagagata tttccgggaa
     1201 tccctttcca tcagggtatc cggcctcggc taatagtttc ttcgcccgtg gcagatcata
     1261 ggaatatcct ttgagattcg gattgtagcc gagaattccg ggcggcagaa cgcccttcgc
     1321 cacgcgcgcg cgcccgttca gaatcagatt gacgatcgtt tcgcgatcga tggcgtagtt
     1381 gatcgcctga cgcaccttcg ggttatcaaa aggtggcttg cccacgttga aaccgatgta
     1441 atacgtgccc cagcggctgg tttcctgaaa atgaggtgcc cacgacggat ccgttttcac
     1501 gttcttgtac tccgggtcag gcacgtcgtt gatcagcagt tccccacgtt tgaaggcggc
     1561 aaagcgctct ttttcatcgc gaatgatcgg aaactcgagc gtatcaagat aaggacgacc
     1621 gcggaagtag tcgggattcg ctttgaggat cagcgtttcg cctttccacc ccgcgtaccg
     1681 aaacggaccg gaaccaacgg gaagcgctcc cagtttagga ttgtcgcgac gggcttcatc
     1741 cgccggaacg atgaacgcat tactgagcgc cagcaactgc agaaacgggg aaaagggctt
     1801 ttcgagcgtc atcgacaggg aatcagatgc cagaaccttg attccatcga tgcgtgaggc
     1861 cttgccagtc gcgtagtcgg tagctccacg aatcaccaga aactcctggc cacgctgact
     1921 tttcatcgcc ggatccagca ggcgatggaa ggtgtaatcc acatcttcgg cggtgcagac
     1981 gcgcccggcg ttcagcgtcg acgcatcgtt ctcgccacag acctggtgaa actttacatc
     2041 gcggcgcagt ttgaaggtgt acgtcaaacg atcagcactc acctgccaac tttcggcaat
     2101 caccgggctg acgcgggcct cctgatcaaa ctcgaccaga ccgtcataca tctgtcgaca
     2161 cacttcgtgc gaaaaggaat cggcgacctc gcgtggatcc agtgacagac cggtactcgc
     2221 aaaagcacgg cggaacactc cgccctgcac aggcgtcacc tcgaggggct tggttgcctc
     2281 gggcttcggt gcatcgccgc acccgacggt acagagaagc atcaaaagaa accatcgact
     2341 gaacactcgt cccgtctccc gcttcacgtt ggctttcctc gtttctttgc aggctcgctg
     2401 tcccaggggc tcgtacacaa gggcggtatc ataccatgat cccccaccca gagcaacgag
     2461 cgcggcaggc tgcgagcatt tgaatctgtc cagttttcct cttggcgtga gcgaatcttg
     2521 tgcgctgtcc ggcaatatgg ggtcttgcga agccgcttcc ctgtgatata atgctctcta
     2581 cagagtgcaa tggaggcaag agtggctcgt ctgatcgcgg tttttctctt tctgacaatc
     2641 ctgatcacat cctctccggt atggtcggcc gagcgatctg atcgtattcc ccaccgaacg
     2701 gtgtatgcca ttcgttgtct tgcgcgtgac aacatctgga tcggaaccga ggacggtgct
     2761 gcacgcttcg atggtcgcac ctggaccatc taccgcacgg ctgacggtct gccccaccaa
     2821 gtcgtcacca gcatcgccga agacccgcag gatggcactc tgtggttcgg cacgttcggt
     2881 ggcatcgccc gattccggaa cgagcgtttt gaatccttct ctaccagcaa ttccggactc
     2941 atcaatgatg tggtctatgc ggtcactgtt accccgccgc acgtctgggc cgccacgacc
     3001 aacgggctct cgcgctttca cccgcagacc cgcgaatgga agagcttcaa cgaccagaat
     3061 gccccgatgc acgaagtctg gtgttatggc gctacgaccg aagcgaccac gggtgaaacc
     3121 tggtttgcca tctggggaag cggcattctg cgagtgaaag cgaacgggga cttcgtcagc
     3181 tataccgacc ccgatgaaat cttcgacgtc gacacggtgc gcaatgatgg cccgttgcac
     3241 gagattcagg tcgcgatcga tacgcatcag aacgtcgtgt attctggagc ctacttcggt
     3301 ctcacccgct tcgataccgc ggccaacaaa tggaacacct ggacaatcaa agatcccgat
     3361 tcgggtctgc cgagtgattt cattaacacg gttcgccttt cccccgatgg gaaaatctgc
     3421 gctatcggca ccgacaaggg gctttgcctg ttcaatcatg atcgtcaatc gtgggtcacg
     3481 tatcggcgct cggcacaggg tggcggcgag tatcgcatct tcgaaaaggg agcctggcgc
     3541 accgaatcgg cacctcaggc cccggccgga gacttcatct ggtcgatcga ctttctcgac
     3601 gatacgatct gggttggcac ggccagcggc ttgtacacgg ccagcatcaa acagtagcgg
     3661 agcacgacca tgaaccatcg aaaagcctgt ggtttcgttc tgtttctgat tgccatctcg
     3721 atttgcggca gcgccatcgc tcagccgttg aatgcctacc accagtggac cgaccccttc
     3781 gagtaccacg gaccggcacg cgaggtcacc gagggcgaac agaatccgcc gacggtgaag
     3841 gtgggcgtct tgtgctcgct cagcgggaat ggcgaagaat atggtcaagc catgaagcaa
     3901 ggggccgaca tggcgacaga cgagatcaac cgcgccggcg gcctgctcgg acgcccgctc
     3961 gagttggtct atcgcgatga tcgcaacgat atgggcgaaa acgggcatca gaccgtcaag
     4021 atgatcttcg atgacaaggt ctgggccatc atcgggtccg tgcattccgg ctgcacccat
     4081 gtcgccgccc gcgtgaccct gaaagccgag acgccgcaac tgacgacggt gtcaaccgat
     4141 ccgacggtgc agatgatcgg cagtccctgg atgttccgct gcctggccga tgacaaggcc
     4201 cagggaaccg caatcgcgaa cgtggtcttc gaccgccaga aactgcttcg cgtcggactt
     4261 tttcagcaaa aaaaccggta cggaaaaatg ggcggcaaga cgatcgggca gatcgccgag
     4321 catcgcggca aaccgctgat cttcaagaat ttctttgaaa gcggacaaac ggacttttcc
     4381 gcacaagtac aagtggcaaa gaattcaaac gccgacggca taatcatctg gggcttatat
     4441 caagaagcag gcttgctggt aaaagctctg cgcgacgcgg gcctcaaggc ccagatcttc
     4501 ggcgcggatg gcatggtctc accggggttc attcgtatcg ccgggacctc cgccgagaac
     4561 gtcatcgtca cctatccgtt tgatgacacg aacccggccc ccgccactca gaagttcatc
     4621 gccgacttcg aacggcggta cgggcaccgc cccgattcgt tcgccgcgca cgcctacgac
     4681 gccgcctgga tgctgatcta cggcttcgcc tgggccgagt accaagagga cagcgtcgcc
     4741 tccggcctca ccctggcccg cggcctgcgt aagctctccg cccccggcgt gcccgccgcc
     4801 gaccaggtcc ccgtgcgccc gtcgagctgg aacgaggtca aggcgagctt ctccgacggc
     4861 gcggcggtga acctgctcgg cg
//
LOCUS       DAORHD010000175         4881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18187_length_4881_cov_3.972234, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000175 DAORHD010000000
VERSION     DAORHD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18187_length_4881_cov_3.972234"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PKO06_05055"
     CDS             <1..691
                     /locus_tag="PKO06_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69047.1"
                     /translation="ELAESRLQLARELGLRNSDTLTCVGSLSPQLVLPEFDTLAESLL
                     CSHPDLRDLDLNRADTRAERTLLQAEKRPTWSVGAGIRQFREDRAHGFLVSIETELPD
                     RRLHRGRLQALERADQKTAAQRHTLIEQLTQLLREQCVRFANARTQALEISEHILPRS
                     RELRDMALEAYQSGKTDLLELLVARKDALEASRQHLSALSDLYQAADAIEQLCGMCLV
                     GESHHSPTHKH"
     gene            803..1102
                     /locus_tag="PKO06_05060"
     CDS             803..1102
                     /locus_tag="PKO06_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69048.1"
                     /translation="MMKQRHLMISRRLTPDMIMQPPSLSTTMTDTNMQRLSQSLTMIM
                     LDMPMRYPSLATIMPGTHMKHASVTPCPTRTSVWPLVNIECPPSIATPVGMRSGW"
     gene            1096..2172
                     /locus_tag="PKO06_05065"
     CDS             1096..2172
                     /locus_tag="PKO06_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV69049.1"
                     /translation="MVKVGSQALALLSFERLQTASAAESIEVNGELAFDENRVRCLTA
                     PVSGRVASVSLRVGDAVREGQTLLVLQSPELAQAALDLLKKQSELDLAGKKRDREARL
                     REKKIASEQEKQEAEAAWELAKIEFDNARRRLLIFGVSEKRVAALTRQTADPVFRGEL
                     AIPSPTSGRVIFRDVNIGDTVSSDKELLKVADLTHLQGIAQAHEKHVAMLLSTLRQRT
                     VAAEVRADAFPGQLFKAAVVSPGPAITVETRTLPLRLDIENPEETLRPGLFFQARINL
                     TDRQPQSTLSREAVLSDEGKSFVFVRFRDDLFLRRPVTTGRVDGSKVGIIDGVQPEQE
                     VVSNGAFLLKSDVLREKMGAGCAD"
     gene            2182..>4881
                     /locus_tag="PKO06_05070"
     CDS             2182..>4881
                     /locus_tag="PKO06_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HNV69050.1"
                     /translation="MYQFTEFLIGNRRLILVFSLLIIVGGYWSWDSLPIDAFPDVTNI
                     QVMVLTKAPGLAPLDVERQITFPIEQRMSGMKGVIQVRSSSKGGLSQIVVVFDDDSDP
                     YFARQLVFERLQEAREELPAGVEPEMAPLSTGLGEVFQYTLDSSTVDATELRTIQDWI
                     VAPQLRTIANISEVNSFGGFVKQYQVLPNPDKLQQYKISLEEIITALERNNTNVSGNF
                     ITRGWEQFYVRALGLLQTPEDIGNIVLKTVDGTPVHVHDLAEVKLGHMTRQGAVTRDG
                     KGEAVAGIAIMLRGSNSKTVVDAINRRIPGIRASLPPGVSLNVFYDRTHLIRECVKTV
                     INALNEGGILVVVVLFLFLAEWRMGLLVFLSLPFSFLLCFIAMKYLGVSANLMTLGGL
                     AFSVGTVCDPTIVVLENIRRHFVERGQTVSPRETVPQAVAEVGRPTAFSELIIILVIV
                     PIFALQGFEAKMFTPFATTLLLAMASAMIVSFLLMPVFGYYFVPRGEESEFFISALTR
                     RLYQFVLDLSLRVPVVPIVIGLLLAGIAWQMLGRLGTDFMPSLKEGAIAINVVRLPNA
                     NLEGAVQVSTAMEKLLLEKFPEVEAVVSKTGRAEISEDPMGPEQTDIIITLKPPVPGQ
                     SWRTQDQLVAEIQKELGQFPGLRYAFSQPISLRVNELISGIKSDVAVKVFGDDIETLS
                     GISKRVADVVGKVSGAVDVRAAQVSGMEQIDIDIDREAAARHGVNVSHIQDLIEAGIG
                     GKIVSHIIEGQKRFSLAVRFPETFRGSEEAIGKLRVLTPVGSLLPIGQFVRFHTVQTP
                     IEVARESGRRRMVVETNVRGRDLGGFVTELRNKLQPLQKELPDGYYIEYGGQFENQER
                     AMERLKIVVPISLVLILVLLIMAMGKLSDAFLVLLNLPFAVVGG"
BASE COUNT          988 a         1460 c         1384 g         1049 t
ORIGIN      
        1 cgaactggcg gaatcgcgcc tgcagctggc gcgggaactg ggacttcgca actccgacac
       61 gctcacctgc gtcgggtcgc tctcgcctca actggtactg ccggaattcg acacactcgc
      121 cgagagcctt ctctgctctc accccgatct gcgcgatctc gacctgaatc gagccgacac
      181 cagagccgag aggacactgc tgcaggccga aaaacgaccc acgtggtcgg ttggtgccgg
      241 aatacgccag ttccgtgaag atcgggcaca cggctttctg gtgagcattg aaaccgaact
      301 gccggatcgc cgcctgcacc gcggtcgcct gcaggctctg gagcgcgcgg accagaaaac
      361 cgcagcacag cgacacacat tgattgagca actgacacaa ctgttgcgcg agcaatgcgt
      421 gagattcgcc aacgcccgca cgcaagcgct ggaaatcagc gagcacattc tgccacgttc
      481 gcgcgaactg cgcgacatgg ctctcgaagc gtatcagtcg ggcaaaaccg atctgctcga
      541 actgctggtt gcacgaaaag atgctcttga agccagtcgc cagcacctga gtgcgctttc
      601 cgatctctat caggccgctg atgccatcga acagttgtgc ggcatgtgcc tggtcggaga
      661 atcgcaccac tctcccactc ataaacactg aggtcatcac ttatgaacac tcggactttc
      721 actttgattt cattattcat tctctgtctg ctgtgcttcc agcagggggt cgttccccac
      781 ctgatttgtg cggaagagca tcatgatgaa gcagcgacat ctcatgatca gccgtcggct
      841 cacaccggac atgatcatgc aacccccaag cctgagcacg accatgacgg acacaaacat
      901 gcagcgcctg agccagagcc tgaccatgat catgctggac atgcccatgc ggtacccgag
      961 tctggccacg atcatgccgg gcacgcacat gaagcacgcg tcggtgaccc catgcccgac
     1021 tcggacttcg gtctggcccc ttgtgaacat cgagtgccca ccatcgattg cgacacctgt
     1081 cggtatgaga tcggggtggt aaaagtcggc tcacaggctc tggccctgtt gagcttcgag
     1141 cggctgcaga ccgcctcagc cgctgaatcg atcgaggtca acggggagct ggccttcgat
     1201 gaaaaccggg ttcgttgtct caccgctccg gtcagcggtc gcgtcgcctc ggtatcgctt
     1261 cgcgtcggtg atgcggttcg tgaggggcag acgctgctcg tactgcaaag ccccgagctg
     1321 gcgcaagcag cactcgacct gttgaagaaa cagtccgaac tcgatctcgc cggtaaaaag
     1381 cgtgatcgcg aagcgcgatt gcgcgaaaag aagatcgcct ctgaacagga aaagcaggaa
     1441 gccgaagccg catgggagct tgcgaaaatc gaattcgaca atgcccgccg caggctgctg
     1501 attttcggcg tgtccgagaa gcgcgttgcc gctttgaccc gtcagacggc tgacccggtg
     1561 ttccgcggtg aactcgcgat tccaagcccc acgagcggtc gcgtcatttt ccgcgacgtg
     1621 aacatcggcg atacggtgag cagtgacaag gagttgttga aggttgcaga tctgacgcac
     1681 ctgcagggaa tcgcgcaggc gcacgaaaaa cacgttgcga tgctgctgtc cacattgcgt
     1741 cagcgcacgg tcgccgccga ggtacgtgcc gacgcctttc ccggccagct gttcaaggct
     1801 gccgtcgtct cgcccggacc ggcgatcacc gtggaaaccc gcacgcttcc tctgcgtctc
     1861 gacatcgaga accctgagga aaccctgcgt cccggtctct tcttccaggc ccgtatcaat
     1921 ctgacggacc ggcaaccgca atccacgctg tcgcgcgaag cagtactctc tgatgaaggg
     1981 aagtcgttcg tcttcgtgcg cttccgtgat gatctgtttc tgcgacgccc cgtgacgacc
     2041 ggccgggtcg acgggtcgaa agtcggcatc atcgacgggg tgcagcccga gcaggaagtg
     2101 gtcagcaacg gagcttttct gctgaagtcc gatgttctgc gcgagaaaat gggcgccggg
     2161 tgcgccgatt aggggaaaac gatgtatcaa ttcacggagt tcctgatcgg caaccgtcgt
     2221 cttatcctcg tattctctct tttaatcatt gttggcgggt attggtcctg ggactctctg
     2281 cccattgatg cctttcccga cgtgaccaac atccaggtca tggtgctgac caaggcccca
     2341 ggtctggcac cgctcgatgt cgagcggcag atcacctttc ccatcgaaca gcgcatgtcg
     2401 ggcatgaagg gggtgataca ggttcgctcg agcagcaagg gcggcctgtc gcaaatcgtc
     2461 gtcgtcttcg atgatgactc cgacccgtat ttcgcccggc aactggtatt cgagcgtctg
     2521 caggaagcac gcgaggagct cccggcaggt gtcgaacccg agatggcccc gttgagtacc
     2581 ggtctcggcg aagtcttcca gtatacgctc gacagctcaa ccgttgatgc caccgagctg
     2641 cgcacgattc aggactggat cgtcgccccg caactgcgca ccattgccaa catcagtgaa
     2701 gtcaacagtt tcggcggctt cgtcaagcag tatcaggttt tgcccaaccc ggacaaactc
     2761 cagcagtaca agatctcact cgaagaaatc atcaccgctc tcgaacgcaa taatacaaac
     2821 gtcagcggca acttcatcac gcgcgggtgg gagcagttct acgtgcgcgc cctcggtctg
     2881 ctgcagactc ccgaggatat cggcaacatt gttctcaaaa ccgtcgatgg tacacccgtt
     2941 catgttcacg acctcgctga agtcaaactc ggacacatga ctcgacaagg tgcggttaca
     3001 agagacggta aaggcgaagc cgtggccggt atcgccatca tgctgcgcgg ttcgaactcg
     3061 aagaccgtcg ttgacgccat caatcgccgc attcccggaa ttcgtgcttc gctgcccccc
     3121 ggcgtttccc tcaacgtttt ctacgatcgc acgcatctga tccgcgagtg cgtcaagacc
     3181 gtcatcaatg cgctcaacga aggcggcatt ctggtggtcg tcgttctctt cctgtttctg
     3241 gctgaatggc gcatgggtct gctggtgttt ttgtctctgc ccttttcgtt tctgctgtgc
     3301 ttcatcgcca tgaagtatct cggggtcagc gcgaacctca tgaccctggg cggcctggcg
     3361 ttttcggtcg gcacagtctg cgacccgacc atcgtggtgc tcgaaaacat tcgccggcat
     3421 ttcgtcgagc ggggtcagac ggtcagccca cgagaaaccg tgccacaggc tgttgcggag
     3481 gtcggtcggc ctaccgcctt ttctgaattg atcatcattc tggtcattgt gcccatcttt
     3541 gccctgcagg ggttcgaagc caagatgttc actccgttcg ccaccacgtt gctcctcgct
     3601 atggcgtcag ccatgatcgt ctcgtttctg ctcatgccgg ttttcgggta ttacttcgtt
     3661 ccccgtgggg aggagagcga attcttcatc tccgctctca cacgtcgttt gtaccagttc
     3721 gttcttgacc tgtctctgcg cgtaccagtc gttccgatcg taatcgggct tctgctcgcc
     3781 ggtatcgcct ggcagatgct cggccggctc ggcaccgatt tcatgccgag tctcaaggaa
     3841 ggggcgatcg ccatcaatgt agttcgcctg ccaaacgcga acctcgaggg cgcggtgcag
     3901 gtctcgacag ccatggagaa actgctgctg gaaaagttcc cggaggtcga ggcagtggtg
     3961 tcgaaaaccg gacgggccga gatctcggaa gacccgatgg gcccggaaca gactgacatc
     4021 atcatcacgt tgaaacctcc agttccgggg caatcgtggc gtactcagga tcaactcgtg
     4081 gccgagattc agaaggaact aggacaattc cccggcttac gctacgcttt ttcccagccc
     4141 atatcgctgc gcgtgaatga actgatctca ggtatcaaga gtgatgtggc cgtcaaggtc
     4201 tttggtgacg atatcgaaac tctgtcaggt atttccaagc gggtcgccga tgtcgtcggt
     4261 aaagtcagtg gcgctgtcga cgtacgagcc gctcaggtct ctggcatgga gcagatcgac
     4321 attgacattg accgtgaagc ggcggctcga catggtgtca atgtgtctca cattcaggat
     4381 ctcatcgaag ccggcatcgg tggcaagatc gtttctcaca ttatcgaggg acagaaacgc
     4441 ttttctctcg cggtgcgctt tcccgagacc tttcgcggca gtgaggaagc cattggcaag
     4501 ctgcgcgttc tgacgccggt cggatctctg cttcccatcg ggcagtttgt ccgcttccac
     4561 accgtccaga cgccgattga agtcgcccgc gaatccggac ggcgacgcat ggtggtcgaa
     4621 accaacgtcc gcggtcgtga tctcggcggt ttcgttaccg aacttcggaa caagcttcaa
     4681 ccgctgcaga aagagttgcc cgacgggtac tatatcgagt acgggggaca gtttgaaaat
     4741 caggagcgag ccatggaacg tctgaagatc gtcgttccga tctcactggt actgattctc
     4801 gttctcctga tcatggccat gggaaagctg agcgacgcct ttctcgtcct gttgaatctg
     4861 ccattcgccg tggtcggcgg c
//
LOCUS       DAORHD010000176         4879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18200_length_4879_cov_3.412728, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000176 DAORHD010000000
VERSION     DAORHD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18200_length_4879_cov_3.412728"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PKO06_05075"
     CDS             complement(<1..433)
                     /locus_tag="PKO06_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69051.1"
                     /translation="MTKTDSTPANKRSQKPPMNEGTKLIADLIPFLNLTPSSNDLAES
                     FFASLGSSITTRREAWHQVLCDQDIVSSLDEMRSDVRILLTDPANPAVIDRVNRFMKA
                     LVPRAIPDAGKKSLTLSLIPYDRANDLFTPGQFSCDATGFFQ"
     gene            complement(507..1754)
                     /locus_tag="PKO06_05080"
     CDS             complement(507..1754)
                     /locus_tag="PKO06_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNV69052.1"
                     /translation="MAKRVVPLTDATIRAAIKKRKAGKAIYLFDGHGLYLHVSKTGAT
                     VWNWKFRHKGIPKLKGFGVYPKVTLARARELRSEGERLLTQGINPIDRTRAEKETRSG
                     ANTFESKAREWFLVKKAPDCSPGHADHIKSYLDRDIIPWLGKKAISAVTPDDLLSVAR
                     RVEQRGAIATAHRVIGVCSEIFCYAIGVRNDPTLDVRDKLRKIPKPRHRAAATSESGM
                     IEPQRVGALLRILDGYEGGHIVRCAVRLGPLVFVRPGELRTAQWKDINLDAGEWHLLA
                     SKTNTQHIVPLSRQAMAILRDIFPLTGDKKYVFPNPRSPDGSRPMSENAVLAAYRSLG
                     IGQDELCGHGWRAIARTLLDERLRYRTQVIEKQLAHVVKDQLGTAYNRTEFLDERREM
                     MQRWADYLDELKGAPIENPITVS"
     gene            complement(1924..1997)
                     /locus_tag="PKO06_05085"
     tRNA            complement(1924..1997)
                     /locus_tag="PKO06_05085"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1961..1963),aa:Met,seq:cat)
     gene            complement(2045..2118)
                     /locus_tag="PKO06_05090"
     tRNA            complement(2045..2118)
                     /locus_tag="PKO06_05090"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2082..2084),aa:Met,seq:cat)
     gene            complement(2132..2215)
                     /locus_tag="PKO06_05095"
     tRNA            complement(2132..2215)
                     /locus_tag="PKO06_05095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2179..2181),aa:Leu,seq:taa)
     gene            2685..3437
                     /locus_tag="PKO06_05100"
     CDS             2685..3437
                     /locus_tag="PKO06_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69053.1"
                     /translation="MVALLIMAFAFIPLVGVIGTGATDTDVAKSYIFAQTTARNILDN
                     LLDNVPFECVRVAPGSVSDTDGSNAETAVSQLVSTPEYQVASFLSLLGNPGSVDTFGR
                     GELRDERGTRYFIKLFCFPLAGTANAVGAINPADEITFRYLPRPPYETATNASNQAVW
                     YPTPTAPAPAFVNAGVPRPYDFTVGSTPDFAIRTTNAFSLGVPQGSIPVLNYCVLKRI
                     LLRVRWVMPKGGERSIEIYTAKGNLAREDLAP"
     gene            3434..4162
                     /locus_tag="PKO06_05105"
     CDS             3434..4162
                     /locus_tag="PKO06_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69054.1"
                     /translation="MIHSLPHGLVHLTHRRGMAIPIVLGLALAMAILGAFVINTNKET
                     YRQNITGLAALQAHFVARAGMEHAMVKVKYLERELYDAACLAQGRNPLFDFTRPLHPL
                     YNPGPAFLFYSSQSGAVGLVGSFAASMGSLPGCTTVPGAWVDEFRADMNSGANISGTN
                     VNMVLTMSPLPNDVRSRMREPFNAQYQVTSLNVLANTRNEAANQVTNRVIVDINVEST
                     LTTARGQLFDHNLQKTIRIQRDRR"
     gene            4174..>4879
                     /locus_tag="PKO06_05110"
     CDS             4174..>4879
                     /locus_tag="PKO06_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69055.1"
                     /translation="MQTTPLVRSRGFTLTEILIGAAVMSLFMAGLFLLFRSGQNASAQ
                     AFWIQRISGSLRNTTRHISQTVQKSSYPSTIVFPGNILENTGNDFRLHFTARGTLFAT
                     QSASVAAPGPLGTQFLRFVESFPEKQGFNVNHAASLTYHIYSLSSQGKLYYHRYEENP
                     GQSTPPGYTQLSTLTRPTIPPGGAALRQAQELVDNVESVAVSVPTTTATAPSILVDIT
                     CRYPRGETRRSEQAVVV"
BASE COUNT          990 a         1367 c         1351 g         1171 t
ORIGIN      
        1 gttggaaaaa ccccgtggca tcgcaagaaa actgaccggg tgtgaaaaga tcgttggccc
       61 tgtcgtatgg gatcagcgaa agagtcaggg atttttttcc ggcatctgga atggctctcg
      121 gaaccagggc tttcatgaat cgattgactc tgtcgatcac cgctgggttt gcggggtcgg
      181 ttaacaagat ccggacgtcg gagcgcatct catcaaggct agacacgatg tcctgatcgc
      241 acagcacctg gtgccaggct tcccttcgtg tggtgatcga agaacccaac gaggcgaaaa
      301 agctctcagc aagatcattt gaagagggtg tcaggttcaa aaacggtatg agatcagcga
      361 tcagcttcgt cccctcattc attggaggct tctgactgcg cttgttcgct ggggtgctgt
      421 cagtttttgt catgtgttcc cttcatttca tgctgagcct tcgatcatcg attatgatgc
      481 gacggggtgg tagcgtcaac cccaggctaa gaaacggtga tcgggttttc aatgggggct
      541 cccttcagtt catcaagata gtcagcccaa cgctgcatca tctcccgacg ttcgtcaagg
      601 aactcggtgc ggttatatgc agtgccgagc tggtccttga cgacgtgagc gagctgtttc
      661 tcaatgacct gcgttctgta tctcagacgc tcatcgagca gggtccgtgc aattgcccgc
      721 cagccgtgcc cgcaaagctc atcctgcccg atgccgaggg accggtacgc ggcgagaact
      781 gcattttcgc tcattgggcg cgacccgtcg ggtgaccttg ggttcggaaa aacgtatttc
      841 ttgtcgccag taagcggaaa gatatcgcga agaattgcca tggcttgtct ggacaaaggc
      901 acgatgtgct gggtgttcgt tttcgaagcc agcaggtgcc attcaccggc gtcgagattg
      961 atgtccttcc actgggcggt gcggagctct ccgggtcgaa caaagaccaa tgggccaagg
     1021 cgaacggcac aacggacgat atgcccgccc tcgtatccat ctaggatccg cagcagcgca
     1081 cccaccctct gcggttctat cattccactt tcgctggttg ccgcagccct gtgacgcggc
     1141 tttggaatct ttcgcagttt gtctcggaca tcgagggttg ggtcgttccg aacgccaatc
     1201 gcgtaacaga aaatttcgct gcaaacaccg ataacgcgat gagccgtggc gatagcgcct
     1261 cgttgctcca cccgccgtgc aacggagagg aggtcatcag gggtgacggc tgatatcgcc
     1321 ttcttcccca gccacgggat gatgtctcta tcgaggtagc ttttgatgtg gtctgcgtga
     1381 cccggcgagc agtccggtgc ctttttgaca agaaaccact ctcgggcttt cgactcgaat
     1441 gtgttcgcgc ctgatcgagt ttctttctcg gctctggttc ggtcaattgg atttattccc
     1501 tgcgtgagca gtcgctctcc ctcgcttctc agctctctcg cgcgagccag ggtgactttt
     1561 gggtataccc cgaacccctt gagtttcggt atacctttgt gccggaactt ccagttccag
     1621 acggttgcgc cggttttgct gacatggagg taaaggccat gcccgtcgaa taggtatatc
     1681 gcctttccgg cctttcgctt ttttatggcg gcccggattg tcgcatcagt taaggggacg
     1741 acgcgttttg ccatgttttg ggtatatcgc ctttcgttgg ttacctgata tacccataaa
     1801 tatacccaaa attttcagat ttaggtatac ccacgcgggc cgggttggac atccgggcaa
     1861 caaaaaaccc gctcacagag cgggttttaa gactgattga ggtttctttg aacaacctca
     1921 caatagcaag ggagggagtc gaaccctcga catcacgggt atgaaccgtg tgctctgacc
     1981 aactgagcta ccttgcctca acgaaaaaac cggacactct tgcgagtgcc ggctttgtgt
     2041 tcggttgcgg gggacggatt tgaaccgccg accttcgggt tatgagcccg acgagctacc
     2101 aggctgctcc accccgcgat aactgttcca gtgccgggag ccgggctcga accggcacgg
     2161 gttttacccc accggatttt aagtccggtg cgtctgccta ttccgccatc ccggcggaac
     2221 aggcttcaag ataccaccag gtaccgtgaa agtcaagctg aatttcatcg ttcgccgggc
     2281 gataagcggc agagcctttt gctacgctga tttccccaga tcccgagggt acgttgagcg
     2341 cttgttgtca cgcaccgccg cacgcgactc tcgctgagcg tcacgtcatg tgctcacaac
     2401 aaacgacgag ttgtcgcgtt ggcggggcag accattttgc gaagtatctg acaggtcgcg
     2461 agccgaacca gagagaaatc gggcttcaag atctcgatgc agtgctgctt tgtccacctg
     2521 cgtgttcaac ggtaccgaac ccccgatctg acatttgcac ggaggaggag aacagagtag
     2581 aatggaagtg gatgagagct atcacacgat cgaggggtac ggttttttcc tggggccgct
     2641 catcgcggat ttccaggaga cgtgctattt cactgctcga aatcatggtc gcgctactga
     2701 tcatggcttt cgctttcatt cctcttgttg gcgtgatcgg cacgggcgca accgatacgg
     2761 atgtcgccaa gtcatacatc ttcgctcaga ccacggcacg taacattctc gacaatctgc
     2821 tcgacaacgt tccgttcgaa tgcgtgcggg tggctcctgg cagtgtttct gacaccgacg
     2881 gcagcaatgc tgaaacggct gtttcgcagc tggtcagcac tccggaatat caagtggcgt
     2941 catttctgtc gttactcgga aacccgggct cagtcgatac cttcggacgt ggtgaactgc
     3001 gcgatgagcg tggaacccgt tatttcatca aactcttctg ctttcctctg gccggcaccg
     3061 cgaatgcggt tggggcaatc aatccggcgg acgaaatcac gtttcgctac ctgccccgcc
     3121 cgccgtatga aacagcgacg aacgcctcaa atcaggctgt ctggtatccg acgccgacgg
     3181 ctcctgctcc cgcgtttgtc aacgctggtg tgccgcgtcc ctacgacttt accgtgggca
     3241 gcacgccaga ttttgcgatt cgcaccacga acgcgttttc gctcggagtt ccgcaaggat
     3301 cgattccggt tttgaattac tgcgttctga aacggattct gctgcgtgtg cgttgggtga
     3361 tgcccaaggg cggtgagcgg tcgatcgaaa tctatacggc aaaggggaac ctggcgagag
     3421 aggatctcgc accatgatcc attcactgcc tcatggactc gtgcatctga cgcatcgacg
     3481 tggaatggcg attcccatcg ttctcgggct tgccttggcc atggccattc ttggcgcttt
     3541 cgttatcaac acgaacaaag agacgtaccg acagaatatc accgggcttg ccgctctgca
     3601 ggcgcatttt gtggcacgtg cgggcatgga gcacgcgatg gtgaaagtga agtatctcga
     3661 acgggagttg tacgacgctg cctgtttggc tcaggggcga aaccctctgt tcgacttcac
     3721 gcgtccctta caccccttgt acaatccggg cccggccttt ctcttttatt cgagtcagtc
     3781 tggcgcggtc ggattggtcg ggtcatttgc tgccagcatg ggttctctgc ctggctgcac
     3841 gaccgttcca ggggcctggg ttgatgagtt ccgcgcggac atgaactcag gagccaacat
     3901 ttcgggaacg aatgtgaaca tggttctcac catgagccca ctgcccaacg atgtccgcag
     3961 tcgcatgcgt gagccgttca atgctcagta ccaggtgact tcactcaatg tgctggccaa
     4021 taccagaaat gaggcggcaa accaggtcac caatcgggtt attgttgaca tcaacgttga
     4081 gtctaccttg accaccgctc gaggtcagct ttttgatcat aatctccaga agactattcg
     4141 cattcagcgc gatcgacgtt gatgaaaggt tctatgcaga caactccact ggtgcgatca
     4201 cgaggcttta cgctcaccga gattctcatc ggagcagctg tcatgtccct gttcatggcg
     4261 gggctgttcc tgttgttccg cagtggccag aacgccagtg ctcaggcctt ctggatccag
     4321 agaatttcag gcagtctgcg caacacgacg cgccacattt cacagaccgt gcagaagtct
     4381 tcgtatcctt cgacgatcgt gtttcccggc aacattctcg aaaacaccgg aaatgacttt
     4441 cgtctgcact tcaccgcgcg cggcacgctc ttcgccacgc agagtgcttc ggttgcggca
     4501 ccggggcccc tgggaacgca gtttctgcgg tttgtcgaat cgtttcccga gaaacaggga
     4561 tttaacgtga accacgccgc ctctctgacg tatcacatct attcgctctc ttcacaggga
     4621 aaactctact atcacaggta tgaagagaat cccggccaat caacgccacc cggctatact
     4681 cagctttcga cactgacccg accgaccatt ccgccgggcg gggctgcgct tcgacaggcg
     4741 caggagttgg tggataatgt cgagagtgtg gcggtgtctg tccctacgac caccgcgacc
     4801 gccccttcga ttcttgtcga tatcacctgt cgatatccgc gtggtgagac ccggcggtcc
     4861 gagcaggctg tcgtggttc
//
LOCUS       DAORHD010000177         4864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18283_length_4864_cov_3.492202, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000177 DAORHD010000000
VERSION     DAORHD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_4864_cov_3.492202"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PKO06_05115"
     CDS             <1..1020
                     /locus_tag="PKO06_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HNV69056.1"
                     /translation="ALMAGVIMAWVAKSYGWHTCFYLSSGGMLLGLIAFQIGYPHYNN
                     EADGYKPEKLFGRDLLLPNLVWLVLGTVALGALMVHLFNNPAQTKMAITYLSLAIIVG
                     IFIMAMISPDKQERNSILAILVIIVAAVCFQSYFKQLYNSLTLFVDRVFHKELLGFSL
                     EASFFALVPNSLAVILFAGLFTWMWAKLADRGKNPSIPMKIVFALCFAVASAALFWWV
                     AQGIAASGEKASAWWVVLSIVILTLGELNILPMGLSAVSALAPKRHSSFLMGAWFLGT
                     SLGGYFSGFLSSLAAVDKERITDVAFTAGVYGNLYGTCAVGLAVVAVIMLLITPLVKG
                     LMTQK"
     gene            1022..2959
                     /locus_tag="PKO06_05120"
     CDS             1022..2959
                     /locus_tag="PKO06_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HNV69057.1"
                     /translation="MISRSSPKAKPLRASEVEKQVRALLALSGDDVQRRADLETLARA
                     QPAFAGLTWLWGPELYRRNRLLFRPFILSHFASWQFDPPSSWKNVYWKGDIGARLDDW
                     LRETDRLDDVPLFRRLYQWKISSTSFWKVDPTPLIAELKQRFAAAASSAERAALTLKF
                     DLWFELDEPSAIELYRLDKRAAGPYILKHLPNVWSWWGGAKRQLWKQLYDTALAQNDS
                     DTANALFRKQAPLDEWQKRVEQLCKNAPRQTSLVGELRVGETADLCDQLEKIHPEGWG
                     LNLGDTFARMLMISGLDAWPYIRRHLFQIHQGLLFKTAYDTILEYARGMRWWQVWAAL
                     LKTRGGQKEFNREINLLLQDQDSKPEAVQERLLLLTGVAQEWNFGGFGMAQIKPLDDD
                     TALAMYEKFPHLLRGPFRKHLVFTYGLTYPRCLDRFIAAHEDDLIDYLASRAATRLGG
                     WGGKDAVDVAEKLSAVYEALRDNPTEFARRSASVLGQIPAFAIYGYGTLIQKNRLARL
                     LFERSLSSWLDDQVAVTNLVEASEIHVQQLSYRVLGQRDPRAPHIAHANLPLLLGTLL
                     RPLHRRTRTYAFSALENAAIDSEAARLILDKARQALDLPDTHYPKEALLGLMARLLHR
                     WPSLRENAEQPTVYRIDEASA"
     gene            2956..4566
                     /locus_tag="PKO06_05125"
     CDS             2956..4566
                     /locus_tag="PKO06_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69058.1"
                     /translation="MNVWFDYATPSVLESTRDEALIGLTANQRRPVRFHAKLTRNPFL
                     FRVAMRAFGQIIWSSDSWYSSGDTLDPIITVHPDALVFEAFSRDQSVYAQVKLAIDQF
                     EVEGEVKYGTTNVDFTAWLWAALGELRSHRETWLRIDKQGFEVATRQAGGRFEKKVDV
                     PDAWVRGFLNLQQALLIPGPRVHARPVDLLAPIRFLRYAKCRISPRALRYEFEPGQPV
                     RLLLEPWEKAFLFKGSTHHYTELKTIRNWGRRRLALIEPLLPFATDVEIFLKGRSMPA
                     FYLVRLPGMTFTLGLSGVSDHAWTDLESFSASAAAQSIDSRVFEAAFTAFQSRSTLSL
                     AELESESSIARQDALAACSRLCRRGQLTFDLDNGKYRFRKLVDAPVDEQKIFPPSRRF
                     ELALKRVQEEKIRVVSCDMQETRKQHTFKTPEGKLTREVIHRDHHVCGEAVGTGAVEI
                     VVKDNTQIIFGRCQCQQFSDHLLSQGPCEEMISLFLASSDQRRDLPVADEALGPRPPS
                     KRKLREQSEEQGGGDDVDGEESDGVEEE"
BASE COUNT          963 a         1405 c         1432 g         1064 t
ORIGIN      
        1 gcgctgatgg ctggcgtcat catggcctgg gtcgccaaat cgtacggctg gcacacctgc
       61 ttttatctgt catcgggtgg catgctgctg ggactgatcg cgtttcagat tggatacccc
      121 cattacaaca acgaagcaga cggttacaag cccgagaagc tattcggtcg tgatctgctg
      181 cttccgaatc ttgtatggct ggttctcggc acggtagcgc tcggagctct gatggtgcat
      241 ctgttcaaca acccggccca aacaaaaatg gcgatcacgt atctgagtct cgcaattatt
      301 gttgggatct tcatcatggc catgatcagc ccggacaaac aggagcgcaa ctccattctc
      361 gccattctgg tgatcatcgt cgcggccgtc tgctttcaat cctacttcaa gcagctctac
      421 aactcgctga cgctgttcgt cgaccgcgtg ttccacaaag agcttctcgg cttctcgctc
      481 gaagcgtctt tcttcgcgct tgtgccgaac tcgctcgccg tcatcctctt tgccgggctt
      541 tttacctgga tgtgggccaa gctcgcggat cgcggcaaga acccgtcgat tccgatgaag
      601 atcgtcttcg cgctgtgttt tgccgtcgca agcgcggccc tgttctggtg ggtggcacaa
      661 ggaatcgccg cgagtggtga gaaggcctcg gcctggtggg tcgtgctgtc gatcgtgatt
      721 ctgacgctcg gcgaactaaa cattctgccg atggggcttt ccgcggtgag tgccctggca
      781 ccgaaacgtc attcgtcgtt tctcatgggt gcctggtttc tcggcacctc gctggggggg
      841 tacttctctg gcttcctgtc gagcctggcc gcggtcgaca aagagcgcat caccgatgtg
      901 gcctttacgg caggcgtgta tgggaacctg tatggaacct gcgcggtcgg gctcgcggtc
      961 gtcgccgtga tcatgctgct gatcacgcca ctcgtgaaag ggctgatgac ccagaagtag
     1021 catgatcagt cgttcgtcac ccaaagccaa gccactgcga gcctctgaag tcgagaaaca
     1081 ggtgcgggct ttgctggcgc tttcgggtga cgacgtgcag cgccgcgctg accttgaaac
     1141 gcttgcccgg gcgcaaccgg catttgccgg tctgacctgg ttgtggggtc cggaactgta
     1201 tcgacgcaat cgcctgctgt ttcggccatt cattctctcg cacttcgcct cgtggcagtt
     1261 cgaccctccc tcatcgtgga agaacgtgta ctggaaaggc gacatcggtg cgcgactcga
     1321 tgactggctg cgcgagaccg accgtctgga tgacgtgccg ctctttcgac gtttgtacca
     1381 gtggaagatc tcatcgacca gcttctggaa agtggacccg acacctctga tcgctgaact
     1441 gaaacagcgg tttgccgcgg cggcgagttc ggccgaacgc gcggctctga cactgaagtt
     1501 cgatctctgg ttcgaactcg atgagccatc ggcgatcgaa ttgtatcgcc tcgataaacg
     1561 cgcggcgggg ccatacattc tgaagcacct gccgaatgtc tggtcatggt ggggtggagc
     1621 aaaacgtcag ctctggaaac agctgtatga tacagctctg gctcagaacg actcggacac
     1681 ggcgaacgcc ttgttccgca aacaggcccc gctcgacgaa tggcagaagc gggttgagca
     1741 attgtgcaag aatgccccac gacagacatc gctggttggc gagctgcgtg tcggcgaaac
     1801 agccgacctc tgcgaccagc tcgagaagat tcacccggaa gggtgggggc tcaacctcgg
     1861 cgataccttt gccagaatgc tgatgatcag cgggctggat gcctggccgt acattcgccg
     1921 ccatctgttt cagattcatc agggcctgct gttcaagaca gcgtatgaca cgattctcga
     1981 atatgcccga ggaatgagat ggtggcaggt gtgggcggcc ctgctgaaga cacgcggggg
     2041 tcagaaggag tttaaccgcg agatcaatct gctgctgcag gaccaggatt cgaaacctga
     2101 agccgttcag gaacgcctgc tcttgctgac gggagtcgcg caggaatgga acttcggcgg
     2161 gttcggcatg gcgcagatca agccacttga cgacgacacc gccctcgcga tgtatgagaa
     2221 gttcccgcat ctgctgcgtg gcccgtttcg gaagcatctg gtcttcacct acgggctcac
     2281 gtatccgagg tgtctcgatc ggttcattgc cgcgcatgaa gacgatctga tcgattatct
     2341 ggcgagtcgc gccgccaccc gccttggggg ctggggtggg aaggacgccg tcgatgtcgc
     2401 cgagaagctg tcggcggtct acgaagctct gcgcgacaac ccgaccgaat ttgcccgtcg
     2461 gtcagccagt gtgctcggtc agattcctgc ttttgccatt tacggatacg gcaccttgat
     2521 tcagaagaat cgcctcgccc gactgctgtt cgagcgctca ctgtcttcat ggctcgatga
     2581 ccaggttgct gtcacgaacc tcgtggaagc gagcgagata catgtgcagc agttgtcgta
     2641 tcgcgtgctc ggacaacgtg acccgcgcgc cccgcacatc gcgcacgcga atctgccgct
     2701 tctgctcggg acactcctac gaccgttgca ccggcgcaca cgaacgtacg cgttttctgc
     2761 tctggagaac gccgcgatcg attcggaagc cgcacgactc atcctggaca aagcacgcca
     2821 ggctcttgac ctgcctgata cccattaccc caaagaagcc ctgcttggtt tgatggcacg
     2881 gttgctgcac cggtggccgt cactgcgtga gaacgcggaa caaccgaccg tctatcgaat
     2941 cgacgaggca agcgcatgaa cgtctggttc gactatgcca cccccagcgt tctggaatca
     3001 actcgtgacg aagccctgat cgggctgacc gcgaaccagc gtcgaccggt gcgtttccac
     3061 gcgaaactga cacgcaatcc atttctgttt cgtgttgcca tgagagcttt cggacagatc
     3121 atctggtctt ctgatagctg gtactcttct ggcgacaccc tcgacccaat catcacggtc
     3181 cacccggatg ccctcgtctt tgaggcgttc agtcgagatc agagcgtgta tgcccaggtc
     3241 aagctcgcga tcgaccagtt cgaggtcgaa ggcgaggtca agtatggcac gaccaacgtc
     3301 gatttcaccg cctggctgtg ggccgctctg ggtgagttgc gttcgcatcg cgaaacctgg
     3361 ttgcgaattg acaaacaagg tttcgaggtg gcaacccggc aggccggcgg tcgcttcgag
     3421 aagaaagtcg atgtgcctga tgcctgggta cgcggttttc tcaatctgca acaagcgctt
     3481 ctgattccag ggcctcgggt acacgcccgg cccgtcgatc tgctggcacc aattcggttt
     3541 cttcggtatg caaagtgtcg catctcgcca cgcgctctgc gttacgagtt cgaacccggg
     3601 caaccagtac gcttgctgct cgagccctgg gaaaaagctt ttttgtttaa aggttcgacg
     3661 catcactata ccgagctgaa gaccattcgc aactggggtc gacgtcgttt ggcgctgatc
     3721 gaaccgctgc tgccgttcgc gaccgacgtc gagatctttc tgaaaggccg ttcaatgccg
     3781 gcgttttatc tggtcagact cccaggcatg acgtttacgc ttggtctttc cggcgtcagt
     3841 gaccatgcgt ggacagatct tgagagcttc agcgccagcg ccgcagcgca gtcgatcgac
     3901 agtcgagtat ttgaggcggc attcacggcg tttcagtcgc gctccactct gtctcttgcc
     3961 gagctcgaat ctgagagttc catcgcacgg caagacgctc tcgccgcctg ttcaagactc
     4021 tgtcgacgcg gacaattgac ctttgatctc gacaatggga agtatcggtt tcgcaagctc
     4081 gtcgatgccc cggtggatga gcaaaaaatc tttccgccgt cgcggcgatt cgaactcgcc
     4141 ctgaagcggg tgcaggaaga aaaaatacgc gtggtttcct gcgacatgca ggaaacgcga
     4201 aagcagcaca ccttcaagac gcccgaagga aagctcacgc gggaagtgat tcatcgcgat
     4261 catcacgtct gcggcgaagc tgtcggtaca ggcgcggtcg agatcgtggt caaggacaac
     4321 acgcagatca ttttcgggcg ttgccagtgc cagcagttca gcgaccatct cctgtcacag
     4381 ggaccgtgcg aagaaatgat ctccctgttc ctcgccagca gcgatcaacg ccgcgatctg
     4441 ccggtcgctg atgaggccct cggtcccagg ccgccgtcga aacgaaagct gcgtgaacag
     4501 agcgaagaac agggtggggg agacgacgtt gacggcgagg aatcagacgg agtagaagaa
     4561 gaatgaagag tcggcggtgt gcagttagcc ggatgttcca tggtgtttcg ccatggagga
     4621 cgcttgagac ctctcctaca tcgccagggt tcccatcgtg cgaaacgatg ggaatccctg
     4681 agtccagagc tttgcaggta accggccgcc gactcttcgc tctttctcac gcggggaggc
     4741 gctgacatgg gtcttctcga ctggctcaaa agtctcttca ccccgacggt cacgcgtgat
     4801 atatcccgga ttgaagtgcc cgagaacctt gtcgacgtgg tggctgaaac cgtcgatctg
     4861 agcg
//
LOCUS       DAORHD010000178         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18362_length_4847_cov_3.823664, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000178 DAORHD010000000
VERSION     DAORHD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18362_length_4847_cov_3.823664"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKO06_05130"
     CDS             complement(<1..802)
                     /locus_tag="PKO06_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69059.1"
                     /translation="MRLRVIVGIVLVCLALNPVFAFDGVAPDHKSFRYQTLVFNNVHQ
                     FWVVNELYAQMAQQGEATDKNVVDKYNQACEIYQKQSAELGRVILSTLDDNREIISLV
                     SDIYKSFDTNSRVALYPALNVVFQAVVAEWRGPLTEAEFREYFPGYGYTEPGFKYRKG
                     REIDREYKGQTWQSEEQSVTTTWNVELTVTLDVLNILKGWLSGGIIKNLVVKEQTTTS
                     MGGGEPMIVFKVTFQLVKAIVTKTNRKFEVNKIWFELLRAKSSGYSTGL"
     gene            complement(984..3749)
                     /locus_tag="PKO06_05135"
     CDS             complement(984..3749)
                     /locus_tag="PKO06_05135"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69060.1"
                     /translation="MPARKGKTREQVLIELFTRLSKAIRHDGDYFSHLTALFLQGLTS
                     HPPDLLTAVSPRRRRKRTTLDHALSFIYHPLEKCRPTQEITWNNLPLTISTPEKTLID
                     LVQDLDYSPPIGELAQMFASLPYHLPTLLQLARSFSDTVHKRVLFLTAWAGRAALEDL
                     PFADLTRTPIKLDPRETEDLAWDGRFYTKYPRFLLTLPVPSAPRHEDRELREFMELRG
                     FPPVIEALTRRGSFVIRKDTERRSVAWFDEFAKTWFLSLCPLKLEEFLIFSVTGKTTP
                     DVTPVPAALRDWLFQQERAAHLRSRDIRAWVTINLAASNLDRLETAMFWGMRVGLADS
                     IVPVLRSQGHYLFNAGRLPTLQKITTELLSSQGPMPPHVYVLAGRCLARESRFEECLS
                     ILDRGKEETLSGHTVHEDYGDLACVTGQVFRQLHKYPQALTEFFVAREWYIEHKDHAR
                     LSMAEAGIGNVFFCQGNAPGARSHYLAALTAIKHAKQPHAEASILGNLGSVEYDSGRL
                     RRALLFLNRALALHHMLKNKWNISVLGGSRAKILLTMGYFTKSMRGFQEVHRLKQELN
                     HPAGVSEAAALLAWICELMGRSAAAKSWWSLLPAPETLAREPRVHFLIAALRAMTALY
                     QNRLTEARDQYHELYELARRKDASEVESGDSLHGIGVCEALLQNPQAPQLLQRAMDAL
                     GGNPGRIMLAQLRIFARVFYPDTFPSIDLADQLQRYIDSQAYDPFWALYAQSLLGTGI
                     PEAQRFLAYHLRKTPPSMLQTQLRIAPTLYKVLQKVEKRNLRAAEFLSHIDEEGISPV
                     HIDQYRQKHGQVPEKTFVFDGPIGIVHFGSRRGYLKPRSIPHGILSNLLLAYPQPIET
                     EALYRSVWGMEFDAECDLPAIKSALQRVRRLLKSLTPAVRLVRCLSPSDLGAIRIAFS
                     CPWEAIV"
     gene            complement(3771..>4847)
                     /locus_tag="PKO06_05140"
     CDS             complement(3771..>4847)
                     /locus_tag="PKO06_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69061.1"
                     /translation="VLRLGRRALAMRMHDEDLEARVHCLVNTCVFLRRVQREDLNLEF
                     TRYLLSSGTAHHINWMTLEGFRHLCWNAEKTANRTVHQVLEHYLSQLSQDEKLRQETG
                     AQQIIERGFDLMLRHQQILEHRRSRSDMTKQLVRAIENQDLALAEQMLRNGADPDGLE
                     PAPEAEPVFPPLHRAIQLENLELCRLLISFGADIHDQERTGGYSPLSIAVGLGNATLA
                     ALLLAQGADPNKKTVPGQTLLMRAVIEQHSQMIALLIDQGAFTEARDDVDNTVLQLAA
                     SEGTPRALQLLIDAGADANITNDQGQGLIHLAAMNGRFENVQVLIQYGFNAAGPDKQG
                     LLPIDWARKGNHQTVVDLLTEHAS"
BASE COUNT         1022 a         1283 c         1517 g         1025 t
ORIGIN      
        1 gtaggccggt tgagtagccg ctggacttgg cgcgcagcag ttcaaaccag atcttgttca
       61 cttcgaattt gcggttcgtc ttggtgacaa tggctttgac cagctggaac gtgaccttga
      121 agacgatcat tggttcaccg ccacccatcg acgtcgtggt ctgttctttg accacgaggt
      181 tcttgatgat gccgccggac agccaaccct tgaggatgtt gagaacatcg agagtaacgg
      241 tcagttccac gttccaggtc gtggtcacgg actgttcttc ggactgccag gtctggccct
      301 tgtattcgcg atcaatttcg cggcccttgc ggtatttgaa accgggttcg gtgtacccgt
      361 agccggggaa atattcgcgg aattcggctt cggtcagggg accgcgccat tcggccacga
      421 cggcctgaaa caccacgttc aatgcgggat acagggcgac gcgggaattc gtatcgaagg
      481 atttgtagat gtcgctgacg agagagatga tttcgcggtt gtcgtcgagg gtgctgagaa
      541 tgacccgacc gagttcagcg ctctgcttct ggtaaatttc acaggcctgg ttgtacttgt
      601 cgacgacgtt tttgtcagtg gcttcacctt gctgagccat ctgagcgtac aattcattca
      661 cgacccagaa ctggtgtaca ttgttgaaca cgagggtctg atagcggaaa ctcttgtgat
      721 cgggcgcgac gccgtcaaac gcgaaaaccg gattcagagc gagacagacc aggacgattc
      781 cgacgataac tctcaaacgc atgtgacata ctccttacta ctcgatttac tcagtgagag
      841 tgatacgacc tagattaact atatggaatt cttcgggttc aatcaatgga tctgcgggtt
      901 gcaggtattg tgcgtcggcg acggggtgtg agagcgggtt gcgcgcccgg atttgagaaa
      961 atgttacaaa agatatgcgg cgatcagacg attgcttccc aggggcacga aaatgcgatg
     1021 cgaatcgcgc ccaggtcgga agggctcagg cagcgcacga gtcgcaccgc cggagtcagc
     1081 gatttgagca ggcgccgtac tcgttgcagc gcgctcttga tggcagggag gtcgcactcg
     1141 gcatcgaatt ccattcccca gaccgaacgg tacaacgcct cggtctcaat gggctgaggg
     1201 tacgccagca gcaggtttga caggatgcca tgcggtatcg agcgtggctt gagatagccg
     1261 cggcgtgacc cgaaatgaac gataccgatc ggtccgtcga agacaaaggt cttttcgggc
     1321 acctggccgt gtttctgtcg gtactgatct atgtgtaccg gcgagattcc ctcttcatca
     1381 atatgcgaga gaaattcggc tgcgcgcaga tttcgttttt ccactttctg cagcaccttg
     1441 tacagcgttg gtgcaatgcg aagttgcgtc tgcagcatcg atggaggagt ttttctcagg
     1501 tggtatgcga gaaacctctg ggcttcagga atgccggtgc ccaggaggga ctgcgcatag
     1561 agcgcccaga aggggtcata ggcctgggag tcgatgtacc gttgcagttg atcggcgaga
     1621 tcaatagagg gaaaagtgtc gggatagaag acgcgggcaa agatccgcaa ctgcgccagc
     1681 atgatccggc cagggttccc gcccagagca tccatggctc gttgcagcaa ctgcggggcc
     1741 tgcgggttct gcagcagcgc ttcgcagacg ccgataccat gaagcgagtc gccagattcc
     1801 acttctgaag cgtcttttcg ccgcgccaac tcgtacaatt cgtgatactg atctctcgct
     1861 tcggttaagc gattctgata caaggctgtc atggctctca gcgcggcgat gagaaaatgc
     1921 actcgaggtt cgcgtgccag cgtctcgggc gcgggaagca gactccacca gcttttggcg
     1981 gcggcagaac gtcccatgag ttcgcagatc caggccagaa gagcggcggc ctctgaaacg
     2041 ccggccgggt gattaagttc ctgcttgagt cggtggacct cctgaaaacc gcgcatcgac
     2101 ttggtgaagt agcccatggt gagcaaaatc ttggcccgac tcccgcccaa tactgagatg
     2161 ttccacttgt tcttcagcat gtgatgcaaa gcgagggcgc gattcagaaa cagaagggca
     2221 cggcgtagac gcccggaatc atattccacg ctgccgagat ttcccagaat ggaggcttcg
     2281 gcatgcggtt gcttggcgtg tttgatggcg gtcagggcgg caagatagtg agaccgggcc
     2341 cccggcgcat ttccctggca gaagaagacg tttccgatgc ctgcttcggc catcgagagg
     2401 cgggcgtgat ccttatgctc aatgtaccat tcccgggcga cgaaaaactc ggtcagagcc
     2461 tggggatact tgtgaagttg tcggaacacc tgcccggtga cgcacgccag gtcaccgtaa
     2521 tcttcgtgaa ccgtatgacc gctcagggtt tcttcttttc cgcgatcgag tatcgacagg
     2581 cactcttcga aacggctttc gcgggcgaga cagcggccgg caagcacata cacgtggggc
     2641 ggcatgggcc cctgtgagct gagcagctcg gtggtgattt tctgaagcgt ggggagccga
     2701 ccggcattga agagataatg accctgagaa cgcagcacgg gaacgatgga atcggcgagc
     2761 ccgacgcgca tgccccagaa catggcggtc tcgagacgat caaggtttga ggccgcgagg
     2821 ttgatcgtca cccaggcacg aatgtcgcga gaacgcaggt gagcggcgcg ctcctgctgg
     2881 aagagccagt cgcgcagagc ggccggaacc ggggtgacgt ccggggtcgt cttcccggtg
     2941 acggagaaga tcaggaactc ttcaagtttc agcggacaaa ggctcagaaa ccaggtcttg
     3001 gcaaactcat cgaaccacgc gacagagcgt cgctccgtgt cttttctgat gacgaatgag
     3061 ccacggcggg tgagggcctc aatgacgggc ggaaagcctc gcagttccat gaactcccgc
     3121 agttcacgat cttcatgacg cggagctgag ggaacgggga gggtcagcag aaaccgagga
     3181 tacttggtat agaaacgtcc gtcccaggcg aggtcttctg tctcgcgggg atcgagtttg
     3241 atcggggtgc gggtgagatc ggcgaaggga aggtcttcga gcgcggcgcg cccggcccag
     3301 gccgtgagaa acagaacgcg cttatgcacc gtgtctgaga agcttcgggc cagttgaaga
     3361 agagtcggca gatggtacgg aagcgatgcg aacatctggg ccagttcgcc gattggcgga
     3421 ctgtagtcga gatcctgaac cagatcgatc aaggttttct caggggtcga gatggtgagc
     3481 ggcagattgt tccaggtgat ctcctgcgtt ggtcgacatt tctcgagagg gtgataaatg
     3541 aacgacagcg cgtgatcgag agtggtgcgc tttcgccggc gccgtgggga gacggcggtc
     3601 agcaggtcag gggggtgcga cgtgagacct tgcaggaaca gggccgtgag gtgggaaaag
     3661 taatcgccgt catgacgaat cgccttgctg agacgcgtga aaagctcgat cagaacctgc
     3721 tctcgtgtct tccctttcct ggccggcacg tgatctcctc ctgaatgagg tcaggatgcg
     3781 tgttcggtta acaagtcgac gacggtctga tggtttccct tgcgggccca atcgatgggc
     3841 agcaatccct gtttgtccgg tcccgcggca ttaaaaccat actggatcaa tacttgcaca
     3901 ttctcaaacc gaccattcat ggcagccaga tggatcaggc cctgcccctg atcattcgtg
     3961 atattcgcgt cggctccggc atcgatgagc agttgcaagg cgcgtggtgt cccttccgaa
     4021 gcggcgagct gcagaacggt gttgtcgacg tcgtcgcgcg cttcagtaaa cgcaccttgg
     4081 tcgatcaaca gggcgatcat ctgactgtgc tgctcgatga cggcccgcat cagaagcgtc
     4141 tgcccgggaa ccgttttctt gttcggatct gcaccctgcg ccagaagcaa cgcggcgagc
     4201 gtcgcgttgc cgagaccaac cgcgatcgac aggggggaat aaccgccggt tctttcctgg
     4261 tcgtggatat ccgctccgaa tgagatcagc agccggcaga gctcgagatt ctcgagttga
     4321 atggcccgat gaagcggagg aaagaccggc tcggcctccg gtgcgggttc cagaccatcc
     4381 gggtcggccc cgttgcgcag catctgctcg gccagcgcga gatcctggtt ttcgatggcg
     4441 cgtacgagtt gctttgtcat gtcagagcga ctgcgccggt gttcgagaat ctgttgatgt
     4501 cgaagcatca agtcgaagcc tcgctcgatg atctgctgcg cgccggtttc ctgccgcagt
     4561 ttttcatcct gcgacaactg gctgagatag tgctcgagca cctgatgaac ggtccggttc
     4621 gcggtttttt ccgcgttcca gcagagatgg cgaaagccct cgagtgtcat ccaattgatg
     4681 tgatgagcgg tccccgagga aagcagataa cgcgtgaact cgagattcag atcctcgcgc
     4741 tgcacgcgcc gcagaaagac gcaggtgttg accaggcagt gcacgcgagc ctcaagatcc
     4801 tcatcgtgca tgcgcatggc cagggctcga cgcccgagtc gcagaac
//
LOCUS       DAORHD010000179         4838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18407_length_4838_cov_3.125653, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000179 DAORHD010000000
VERSION     DAORHD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18407_length_4838_cov_3.125653"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1979
                     /locus_tag="PKO06_05145"
     CDS             <1..1979
                     /locus_tag="PKO06_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNV69062.1"
                     /translation="QELETRRTSIIGSLTERALLSDDLRQRLESAETLAILEDLYLPY
                     RPKRRTRATMARARGLEPLAERLRAQDPGLDPWAAAGPFVDPEKGVETAEVALAGARD
                     ILAEMISEDANTRQALRQLFEAEGRISSTVVRGKEEEGQKFRDWFDWSEPVTRAAGHR
                     VLAMLRGESEGILRLKIRPADDKALLLLDQRWVKGRSPAAAQMRLAVEDGYDRLLAPS
                     LENEIRQLNRERCEREAIQVFARNVRELLMAPALGQKAVLAVDPGLRTGCKTVCLDPQ
                     GKLLYQTVLYFTGSERHLQDSMKTAASLVDTYHLDVIALGNGTGSREAEAILRRLPFS
                     RPIPIVVVNESGASVYSASEAAREEFPDQDVTVRGAVSIGRRLQDPLAELVKIDPKAI
                     GVGQYQHDVDQNALKRELDDVVRSCVNAVGVEVNTASKRLLTYVSGLGPTLAANIVAW
                     REANGPFQSRKDLQRVPRLGPKAFEQAAGFLRVRQSDHPLDASAVHPERYTLVERMAR
                     DAGSTVYELVQNESRRRQIELSRYVDAEVGLPTLNDILQELARPGRDPRAEFELFAFD
                     DAVHEIEDLTPGMKLPGIVTNVTRFGAFVDVGVHQDGLVHISELADRFVRDPAEVVKV
                     GQRVMVMVLEVDRNRRRIALSLKTGSGASRCSVP"
     gene            2091..4118
                     /locus_tag="PKO06_05150"
     CDS             2091..4118
                     /locus_tag="PKO06_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV69063.1"
                     /translation="MSQTDWSNFGSSGADYVGQPSQDYTSVTLYVSEKLENGPIIAEL
                     RDLGYSVQVFAHGQQDWLQGKTLGIYVIHEDLLIQELLGFLDMIFMGPVESPVVVIGD
                     QMGQDYLSGMFKNRPFFYLPPQVEAGRLGETMRLAADAVLKVKKVRAVEQELDRAEKE
                     IEELNEIGMALSSEKDPDRLLQMILSKCREIAHADAGSLYLVEGGTNLRFKLSQNVSL
                     NWQVSEDILLPINDRSIVGAAALSGKPITLLDAYVIPTSATFSFNRSFDQNTGYRSKS
                     IVAVPMKNHAGEVLGVVQLINRRRDFEQLTPGEKLLEEHIEPFTRKDLNLISSLASQA
                     TIALENSRLVQDIQRLFEGFVHASIVAIESRDPTTCGHSERVAALTVHFAETIDAIGS
                     GPFAGIHFDKPRLTEIRYASLLHDFGKVGVREEVLVKAKKLFPWESENLRKRFALIRK
                     SMEADFYHRSLDLVLERGVDHYRALQVALEANYRMQLQEMDDALAFLLQSNEPTVLEE
                     GNFNRLLEIAKRRYVDLAGQTTEYLNEREMHVLSIRKGSLSEEERIEIESHVTHTFNF
                     LSKIPWTKSLKRVPEIAYAHHEKLNGRGYPNHLEIGAIPFESQLMTICDIYDALTARD
                     RPYKRALPREKALDIMFNEVKQGLIARDLLEVFVQREVFKVIEGRSFRPQI"
     gene            4136..4744
                     /locus_tag="PKO06_05155"
     CDS             4136..4744
                     /locus_tag="PKO06_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69064.1"
                     /translation="MAGQPEILEQIGESFGAQWINDFLTSSGLGILVLLFVTAVPVFL
                     QGPDKVQWRRFLIKCALVTILVVGFLAWHGFDIPKALMHVRWFCYDHYFIVGGLFALG
                     IGFQYTLWYILVPKMVGLAAFSASEWLKNDRLIRSRMVHDLIKKYDDQYVTLKDLKEM
                     LGEPDYIRETDQAMVYDLDCERRLAIFMPTSYKELKIKMVLE"
BASE COUNT         1025 a         1284 c         1501 g         1028 t
ORIGIN      
        1 tgcaggaact ggagacccgg agaacctcga tcatcgggtc gctgaccgaa cgggccctgt
       61 tatcggacga tctgcgccaa cgccttgagt cggcagagac tctggcgatc cttgaagacc
      121 tgtatcttcc gtaccggccg aaacgtcgaa cccgggcgac catggctcgc gcacggggat
      181 tggaacctct cgccgagcgg ttgcgcgcgc aagaccccgg gcttgacccc tgggctgcgg
      241 ctggtccttt tgtcgacccg gaaaagggcg tcgagacggc ggaagtggcc ctggctggcg
      301 cacgggacat actggccgag atgatttcgg aagacgcgaa tacccggcaa gcgctgcggc
      361 agctgtttga ggcggaaggg cgaatttctt cgacggttgt tcgcggcaag gaggaagaag
      421 gacagaaatt tcgcgattgg ttcgactgga gcgagccggt gacgcgtgcg gccggccatc
      481 gggttctggc gatgctgcgt ggagagagcg aaggaatact gcgtttgaag attcgccctg
      541 ccgacgacaa ggcattactg ctgctcgatc agcggtgggt gaaaggccgt tcgccggccg
      601 ccgcgcagat gcggctggcg gtcgaggacg gctatgatcg actcctggcc ccttcgctgg
      661 aaaacgagat tcgccagctg aatcgcgaac ggtgcgaacg cgaagcgatt caggtgtttg
      721 ctcgcaacgt gagagagttg ctgatggctc cggcactggg gcagaaagcg gttcttgcgg
      781 tcgatcccgg cctgcgcacc ggctgcaaga ccgtgtgtct ggacccgcag ggaaagttat
      841 tgtaccagac agtgttatac ttcaccggct cagaaagaca tcttcaagac tcgatgaaaa
      901 cggcggcttc gctggtcgat acgtatcatc tggatgtcat cgctctgggc aacgggaccg
      961 gcagtcgtga agccgaggcc atcctgcgac gccttccttt ttcccggccg attcccatcg
     1021 tggtggtcaa tgaaagcggc gcctcagtgt attcggcatc tgaagcggct cgtgaagagt
     1081 ttcccgacca ggatgtcacg gtgcggggag cagtctcaat cggccgccgt ttgcaagacc
     1141 cgctggcgga gctggtgaag atcgatccga aggcgatcgg cgtcgggcag taccagcatg
     1201 acgtcgatca gaatgccttg aaacgagagt tggacgatgt cgttcgcagc tgcgtgaacg
     1261 ccgtgggagt ggaggtgaat acggcaagca aacgcctgtt gacgtatgtt tccggactgg
     1321 gaccgacgct ggcggcgaac attgtcgcgt ggcgtgaagc caacgggccg tttcagagtc
     1381 gcaaggacct gcagcgggtg ccgcgtctgg ggccgaaagc ctttgagcag gccgccggct
     1441 ttttacgcgt gcgccagagc gaccacccgc tcgatgccag tgccgtgcat ccggaacgct
     1501 atacgctcgt tgaacgaatg gctcgagatg ccggtagcac agtctatgag cttgtacaaa
     1561 acgagagtcg tcgtcgacag atcgaactgt cgcggtatgt cgacgcggag gtgggactgc
     1621 cgactctgaa cgacattctt caggaactgg ctcgccctgg gcgtgatccg cgggccgagt
     1681 tcgagctctt tgcgttcgat gatgcggtcc acgagatcga agacctcaca ccgggcatga
     1741 agcttcccgg catcgtgacc aatgtcaccc ggttcggggc gtttgtcgac gtgggggttc
     1801 accaggatgg cctcgttcac atcagtgaac tggcggatcg ctttgtgcgg gatccggcgg
     1861 aggtcgtcaa ggtcgggcag agggtcatgg tgatggtcct ggaagtcgac cggaatcgtc
     1921 gtcgaattgc cctttctctc aaaaccggat caggtgcttc ccggtgctct gtgccctgag
     1981 attgagcgct gaaaaatcag aagcgttctg agacgaaatg agacccacgg tcatggtata
     2041 cttcgcctga tgagagatca cctgattttt gagaaagaga cattgccaac atgagtcaga
     2101 cggactggag caacttcgga tcttccggag ccgactatgt cggacagccc tcgcaggact
     2161 acacgagtgt gacattgtat gtcagcgaaa agcttgaaaa cgggccaatc atcgccgaac
     2221 tgcgtgatct gggctattcg gttcaggtct ttgcgcatgg acagcaagac tggctgcagg
     2281 gaaagacgct cggcatttac gtgattcatg aagacctgct gattcaggaa ctgctgggct
     2341 ttctcgacat gatctttatg ggcccggtcg aaagcccggt ggtggtcatc ggtgatcaga
     2401 tgggtcagga ctatctttcg ggcatgttca agaaccgccc ctttttctac ctgccgccgc
     2461 aggtcgaagc tggccgtctg ggagaaacca tgcgattggc cgcggacgcc gtgctgaagg
     2521 tgaagaaggt gcgggcggtc gaacaggaac ttgatcgggc cgaaaaagag atcgaagaac
     2581 tcaatgaaat cggcatggcg ctttcctcag aaaaagatcc ggatcgtctg ctgcagatga
     2641 ttctctcgaa atgtcgcgag atcgcgcatg ctgatgcagg cagtctgtat ctggtcgagg
     2701 gtggaacgaa cctgcgcttc aagctgtctc agaacgtatc actcaactgg caggtcagcg
     2761 aagatattct gctgccgatt aacgatagga gcatcgtggg tgccgcagct ctctcgggca
     2821 agcccatcac cctgctcgat gcctacgtga ttccgacctc ggcgacgttt tcgttcaatc
     2881 gcagttttga tcagaatacg ggctatcgca gcaagtcgat cgtcgcggta cccatgaaga
     2941 accatgcggg cgaagtgctg ggagtggtgc agctcatcaa ccgccgtcgc gatttcgagc
     3001 agttgacgcc cggtgaaaaa ctgctggaag agcacatcga gccgtttacc cgcaaagacc
     3061 tgaacctgat ctcgtcactc gcctcgcagg ctacgattgc cctggaaaac tcgcgcctgg
     3121 tgcaggacat tcagcgtctg ttcgaggggt tcgttcatgc gtcgatcgtt gcgattgaat
     3181 cgcgagaccc gaccacgtgc ggtcattccg aacgcgtcgc ggcgctgacc gtgcattttg
     3241 ccgagaccat tgacgcgatc ggtagtggac cgtttgccgg catccatttt gacaagccga
     3301 ggctgaccga gatccgctac gcctcgttgc tgcatgattt cggcaaggtc ggggtgcgtg
     3361 aagaagtgct ggtgaaggcg aagaagcttt ttccctggga atccgaaaat ctgcgcaagc
     3421 ggttcgcgct gatccgcaag tcgatggagg ctgatttcta tcatcgctct ctcgatctgg
     3481 ttctcgagcg cggcgttgat cactaccgcg ccttgcaggt agctctcgaa gccaactatc
     3541 gcatgcagct gcaggaaatg gacgatgcat tggcgttcct gctgcagtcg aacgagccga
     3601 ccgtgctcga agaaggaaac ttcaatcgac tgctcgaaat cgccaagcgc cgctatgttg
     3661 atctggcggg ccagaccacc gagtatctga acgagcgcga aatgcatgtt ctttcgatac
     3721 gcaaagggtc actttcggaa gaagagcgta tcgagattga gtcgcatgtg acccacacct
     3781 tcaactttct gtcgaagatc ccctggacca agagcttgaa gcgggtaccc gaaatcgcgt
     3841 atgcgcatca tgagaagctg aatggacgcg ggtatccgaa ccaccttgaa atcggggcga
     3901 ttcccttcga gtcgcagctg atgacgattt gcgatatcta cgatgccttg acggcccgcg
     3961 accgaccgta caaaagagcc cttccccgcg aaaaagccct ggatatcatg ttcaacgaag
     4021 tcaaacaggg gttgatcgcc cgcgacctgc ttgaggtctt tgtgcagcgg gaagtcttca
     4081 aggtcattga agggcgctcg tttcgcccgc agatctgatc gtacaggaga ggaccatggc
     4141 tggacaaccg gaaattcttg agcagatcgg ggaaagcttt ggcgcgcagt ggatcaacga
     4201 ttttctcacg agcagcggcc tgggaattct ggtactgctg ttcgtgactg ctgttcccgt
     4261 ctttctgcag gggcccgaca aagtgcagtg gcgccgcttt ctgatcaagt gcgccctggt
     4321 cacgattctg gtagtcgggt ttctggcgtg gcacggcttc gatattccga aagcgctgat
     4381 gcacgtgcgc tggttctgtt acgaccatta cttcattgtg ggcgggctgt tcgctctcgg
     4441 catcgggttt cagtataccc tctggtacat tctggtgccg aaaatggtcg gtctggctgc
     4501 gttttcggct tcagaatggt tgaaaaacga cagactgatt cgctctcgca tggttcacga
     4561 cctgatcaag aagtatgacg accagtacgt gaccctcaag gatctcaaag agatgctggg
     4621 cgaaccggac tatatacgcg aaaccgacca ggccatggtc tatgatctcg attgtgagcg
     4681 gcgtctggcc attttcatgc cgacgagcta caaggaactc aagatcaaga tggtgctgga
     4741 gtagccgagc gcgggggcgt cagaacgaga gttccatgcc ctcgcgtgcc agctggacat
     4801 cgagcgtgcg accggcatac tgcggttgag ccagcatg
//
LOCUS       DAORHD010000180         4831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18451_length_4831_cov_3.259631, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000180 DAORHD010000000
VERSION     DAORHD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18451_length_4831_cov_3.259631"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKO06_05160"
     CDS             <1..225
                     /locus_tag="PKO06_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69065.1"
                     /translation="SFSSTRSNNRQEWVDYIAPFAERQTTVCYVNPADPTEAYLDRSL
                     HLVDLAIGTLMTAIFLGCAYLFRFVKGRLA"
     gene            280..603
                     /locus_tag="PKO06_05165"
     CDS             280..603
                     /locus_tag="PKO06_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNV69066.1"
                     /translation="MHPFTSRVLHAIKAVPRGKVATYGLIACLSGNPRGARQVSRILH
                     SCSDRHALPWHRIINAQGGISLNGDGAAIQRALLEAEGIVFTRTGTVDLQCYLWQPDS
                     DFRTS"
     gene            complement(664..2727)
                     /locus_tag="PKO06_05170"
     CDS             complement(664..2727)
                     /locus_tag="PKO06_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69067.1"
                     /translation="MKATWCMGLVLLALTIATNCCQAGEESVKARVTKKLIDHVTRRL
                     EQSLPKTISDDVRRRARAGVALAGSFWRPEDGTPVEFERFCLESFLSDPELERQTFAR
                     IEKQFEVIRGGFHKISRGLREPMDLDLGSMLPIDTLFAEYAPEAHLSEDLFRQKLAFY
                     LLLNWPTVSLEEKIARGPSWKPAEWARARLGDTFTARVPPEVLQKNAEVSVIADTYVN
                     GYNVYPSILEGTGLTKATSAYASDLRLISHWGLRDEIKSLYNRPDALELQKTIFRAME
                     RIADGTIPRAVIDRAEIRWNPVSNQVVEAKSGRAVETTEEGDGRFAILKRVFEAARLL
                     DPYYPAFPTAIDRAFSLERQIPEAAFEKLLTDVVSAPVAHQVGKLIAKRLGRPLQPFD
                     IWYAGFKPRKGLAPEQLDELVRKRYPTNEAFRKDLPNILAALGFSASQATFLSERITV
                     DASRGAGHAMGAQSRDDLAHLRTRVSAQGMDYKGFNIAIHELGHNVEQTWSLHGVEHY
                     SLSGVPNTAFTECFAFLFQARDLEVLGLANATGRDEGERALDAFWSTFEISCVALVDM
                     RVWRWMYAHPDASAADLRSAVTAIAREVWNEYCAPVFGVRDTPILGVYSHMISYPLYL
                     PDYPLGHLVCHQIQSYLQGRDFGTEVERLCRIGCVTPQEWMQKAVGTGLSAEPLVAAA
                     EKAVR"
     gene            complement(2779..4416)
                     /locus_tag="PKO06_05175"
     CDS             complement(2779..4416)
                     /locus_tag="PKO06_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HNV69068.1"
                     /translation="MIVRKLWLGAISLMLVLCLGFSADAATRDEAHEAALSLRRTVLE
                     RESCRVELAPEGQFITVFNDVVGRWVRSELTPELLRGIRSTLAPHLQIRLNARGFAPA
                     AARDTAGKPDATNYDAIWVRDNVWVFYALAANSQHHTEARRLLLALWDYYATSEQIKR
                     FDLIIADPARSLDQMTMPHIRFDGRDPRLGDVRVNGQPEIWNHRQIDAHGLFFTALGE
                     SFLNGLLQPEDLTRERMKVLARYPLFLRRISFDTYEDAGAWEEVPRRNTSSIALATRS
                     LQVWKRLLYKEPAGMQRVRSRFRLLADQMPGLAVEEWGVSCLDELIDRGLKRVRHQLR
                     LGGESPDYEPTDLRYRRADAALLVLIQPSPLEGLTEAELRQVLQIVETLERPAGILRY
                     VNDSYQGGNYWITPEEESTEGPTLTGDTSSDEAFRARLAKLPPHSEAQWFFDSLCALA
                     RLHLAEITRDPRMRRHDLHQAAIHLKRSLGQVTGSNFAADGAPVDAWQTPESINTVEL
                     GGRHILLPSPITPLNWAKAGLDMALRRYAQVAFGAAD"
     gene            complement(4544..>4831)
                     /locus_tag="PKO06_05180"
     CDS             complement(4544..>4831)
                     /locus_tag="PKO06_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69069.1"
                     /translation="PYKEITPKAPAVFALRSIKPPNKKESDSADVGRTLLFFGPYKKE
                     MSGETIQIYQTAREGVGTKVHRLILEVEAEEKVVDELLAKMDLNKLAGLIK"
BASE COUNT         1024 a         1535 c         1314 g          958 t
ORIGIN      
        1 agcttttcct cgacgcgctc gaacaaccgc caggaatggg tggactatat cgcgccattt
       61 gcggaacgcc agaccaccgt ctgctatgtc aacccggctg acccgaccga agcctatctc
      121 gataggtcgc tgcatcttgt tgacctcgcc attggaacgt tgatgacggc gatattcctc
      181 ggatgtgcgt atctctttcg attcgtcaaa ggcagactgg cgtaaggcgt tctaaccatg
      241 caaacctgca cgcgcctgaa accctctgcc gaaaaggttc tgcacccgtt cacgtcgaga
      301 gtgcttcacg ccatcaaagc agtgccaagg gggaaggtcg ccacgtacgg tttgatcgcc
      361 tgtctcagtg ggaaccctcg tggagcgcgg caggtgagcc gcatcctgca ttcgtgttcc
      421 gatcgacacg cgctgccctg gcatcgcatc atcaacgcgc aaggcggcat ctcgctgaac
      481 ggcgacggag cagcgattca acgtgccctg ctcgaagcag aaggcatcgt tttcacacga
      541 accggtacgg tcgacctgca gtgttatctc tggcagcccg actcagactt ccgcacatcc
      601 tagttccgtc tcaggctttc cagaggcgca gaatactcgt catctccctg gacatacggg
      661 cactcaccgc accgcctttt cggcggccgc aaccaacggt tcggcggaaa ggcctgttcc
      721 gaccgccttc tgcatccact cctgcggggt cacgcagccg atgcgacaca gacgctcgac
      781 ttccgtgccg aaatcccgac cctgcagata actttgaatc tggtggcaga ccaggtgccc
      841 aagcgggtaa tcaggcagat acagtggata actgatcatg tgagagtaca cgcccagaat
      901 cggtgtatcg cgcaccccga ataccggagc gcagtactcg ttccaaacct cgcgggcaat
      961 cgccgtcacc gcacttcgca gatcggcggc cgaagcatcc ggatgtgcat acatccaccg
     1021 ccagacccgc atatccacta acgcgacaca cgagatctcg aacgtcgacc agaacgcatc
     1081 gagagctcgc tcaccctcat cccgacccgt cgcgttcgcc agtcccagca cctcaagatc
     1141 gcgagcctga aacagaaacg cgaagcactc ggtaaaggcg gtattcggca cccccgaaag
     1201 cgagtagtgc tcgacgccat gcagcgacca ggtctgctcg acgttgtggc caagttcgtg
     1261 aatggcgatg ttgaagccct tgtaatccat tccctgtgcg gaaacgcggg tgcgcagatg
     1321 cgcgagatca tctcgcgatt gtgctcccat ggcatgtcct gcgcctcgcg aggcatcgac
     1381 cgtgatgcgc tcgctgagaa acgtcgcctg ggaagccgag aaccccaagg ccgcgagaat
     1441 gttcggcaga tctttccgga aagcctcgtt cgtcggatag cgtttgcgta ccagttcgtc
     1501 aagctgttcg ggcgccaggc ccttgcgcgg cttgaacccg gcataccaga tgtcgaacgg
     1561 ttgcagtggg cgtccgagcc gtttggcgat cagcttcccg acctgatgcg cgaccggagc
     1621 cgacaccaca tcggtcaata gcttttcgaa cgcagcttcg ggaatctgac gttcaagaga
     1681 gaacgcccga tcaatcgccg tcggaaacgc tggatagtag ggatcgagca gacgcgccgc
     1741 ctcgaacacg cgcttcagaa tggcgaatcg accatcacct tcttccgttg tctcaacggc
     1801 acgaccgctc ttcgcttcga ccacctggtt gctcaccggg ttccagcgaa tttcggcacg
     1861 atcgatcacc gcgcgcggaa tcgtgccatc ggcaatgcgt tccatggcgc gaaagatcgt
     1921 cttctgcagt tccagcgcat ccggccggtt gtacaaactc ttgatctcat cacgcagtcc
     1981 ccagtgactg atcagcctca aatcagaagc ataggccgaa gttgctttcg tcaagccggt
     2041 tccttcaaga atcgacgggt acacattgta accattcaca tacgtatcag cgatgacgct
     2101 gacctcggca ttcttctgca gcacctccgg gggaacgcgg gcagtgaagg tatcgccaag
     2161 gcgggctcgc gcccactcgg ccggcttcca cgacgggccg cgagcgattt tttcctcgag
     2221 agaaacggtc ggccagttca gcaacagata gaacgccagt ttctgcctga acaggtcttc
     2281 gctgagatgc gcttctggag catattcggc aaacagagta tcgatcggaa gcatcgaccc
     2341 cagatcgaga tccatcggct cgcgcagacc gcgactgatc ttgtgaaacc cgccgcgaat
     2401 cacctcgaac tgtttctcga ttcgagcgaa agtctgccgt tcgagttccg gatcgctcag
     2461 aaacgactcc agacagaacc gctcaaactc gactggagtt ccgtcttccg gacgccagaa
     2521 agaccctgca agagcgacac cggcgcgggc acgccgccgc acatcgtccg agatcgtttt
     2581 cggcagactc tgctccagac gacgagttac atgatcgatc agtttcttcg tcactcgtgc
     2641 cttcacgctt tcctcccctg cctggcaaca attcgttgcg attgtcagag ccagaagcac
     2701 cagccccatg caccacgttg ctttcatctc atcgtctcct tcgctcgcca ccgttcacgc
     2761 gagggcgagg cacatcgctc aatccgcggc tccaaaggcg acttgggcat accgacgcag
     2821 agccatgtcg agaccggctt tggcccagtt cagaggtgtt atcggcgagg gcagcagaat
     2881 gtggcgccca cccagttcga cggtgttgat gctctccggg gtctgccagg catcgaccgg
     2941 agcgccatcg gcagcgaagt tcgaacccgt gacctgaccg agagaccgct tcaggtgaat
     3001 cgcggcctga tgcagatcgt ggcgacgcat ccgcggatca cgggtgatct cagccagatg
     3061 caggcgtgcc agagcgcaca aactgtcaaa gaaccactgc gcctcggagt gcggcggcaa
     3121 cttcgccaga cgcgcgcgga atgcctcatc agaagaggtg tcgccggtca gcgtcggccc
     3181 ttcggtgctt tcctcctccg gggtgatcca gtagttgccg ccctggtaac tgtcgttcac
     3241 gtaacgcagt attcccgccg gccgctcgag cgtttccacg atctgcagca cctgccgcag
     3301 ctccgcttct gtcaacccct cgagcggaga cggctgaatc agcaccagca gcgccgcatc
     3361 agctcttcga tagcgcagat cggtcggttc atagtcaggt gactcgcccc ccagtcgcaa
     3421 ctgatgccgc acccgcttca gtccgcgatc aatcagttcg tcgagacagc tgacacccca
     3481 ctcctccacg gccaggccgg gcatctggtc agccagcagg cgaaatcgcg atcgtacacg
     3541 ttgcattccc gcgggttcct tatacagaag gcgtttccag acctgcaaag aacgggtggc
     3601 caaagcgata ctcgacgtat tccgacgcgg aacttcttcc caggcgccgg catcttcata
     3661 cgtgtcaaag gaaatgcgac gcaggaacag cggatacctc gccagcacct tcatacgctc
     3721 gcgtgtaaga tcttcaggtt gcagcagacc gttcaggaat gactctccga gcgcggtaaa
     3781 aaacagcccg tgcgcgtcaa tctgccgatg gttccagatc tcgggctgcc cgttcacacg
     3841 cacatcaccg agtcgcgggt ctcggccatc gaagcggata tgcggcatgg tcatctggtc
     3901 gagcgaacgc gccgggtccg cgatgatcag gtcaaaacgc ttgatctgct cggaagttgc
     3961 ataatagtcc cagagcgcga gcaacagccg gcgagcctcg gtatgatgct ggctgtttgc
     4021 cgccagggcg tagaacaccc agacattgtc acgcacccag atcgcatcat agttcgtggc
     4081 atccggtttt ccggcggtgt cgcgggcggc ggctggcgca aacccacgcg cgttgagccg
     4141 aatctgcaga tggggcgcga gcgtgctgcg aatgccgcgc agaagttcag gcgtcagttc
     4201 actgcgcacc cagcgcccga ccacatcatt gaataccgtg atgaactgac cctccggtgc
     4261 caactcaacg cggcagctct ctcgctcaag caccgtgcgt cgcaggctca gagctgcctc
     4321 atgcgcttca tcacgcgtcg cggcatccgc ggaaaagccc agacagagga ccagcatcag
     4381 tgagatcgca cccaaccaca gttttctgac aatcatgatt cacctctctc gaaatggact
     4441 cgtcgcattc tactgcttcc aggggaaaag aaaaaccgcc cgcaggttcg tgacctacga
     4501 acggttcaac gtgcctttca gcaccgccac aaagaaaagt gtcctacttg atcaatcctg
     4561 caagtttgtt gagatccatc tttgccagca actcgtcgac gaccttttct tcagcctcga
     4621 cttcgagaat caatcgatgc acctttgttc cgacaccttc tcgggccgtc tgatagatct
     4681 ggatcgtttc gccggacatc tctttcttgt acggcccgaa gaacaagagc gtacggccaa
     4741 catcggctga atccgactcc tttttgttgg gcggcttgat cgatcgcaga gcgaacacgg
     4801 ccggggcttt cggtgtgatc tctttgtatg g
//
LOCUS       DAORHD010000181         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18482_length_4825_cov_3.907128, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000181 DAORHD010000000
VERSION     DAORHD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18482_length_4825_cov_3.907128"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKO06_05185"
     CDS             complement(<1..854)
                     /locus_tag="PKO06_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69070.1"
                     /translation="MMVFFLIAIMRLSFVAAQPSAWMDDVKQLTEGVKRIAKPGVPGP
                     LTCIRENAFPVVLGKAGGHYHPVVAAARMGSGRIVAFGHGGLFETAALQEADTTRLLI
                     NAAHWASRPSHQRSTKPVVGLIFSHDLAKPLEQAGFEVRHLSCPGWEDQIAGIDLLVG
                     DNIVPDRDHEQALNRFIKGGGGFLASGLAWGWMQIHPHLDLTRDYALNRVLAEMGLAY
                     VDGYIDEIPPLSQTDTTALSRAHALNALQRLETASGSEIGQVQALVTTAYRWIPAEEQ
                     TFRDAVRQ"
     gene            complement(957..1844)
                     /locus_tag="PKO06_05190"
     CDS             complement(957..1844)
                     /locus_tag="PKO06_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69071.1"
                     /translation="MKLLKLALLFVLLSHACFGVMIRHDVSESKYLDLANQAGLHAGL
                     VRIPDARGDFSGVLLGPRHILTCGHPILGFLPDGKNEGRVSIAVQVRGKAYQAEYAWL
                     HPRYNRSTHCGGGDLAVLRLTKSVLGTIPARLFSGQIRLGQIFIGVGQGKSGTGKDNN
                     EPLPMGTFRGYANTIDFLFDESELALFRSDFDDGSDGANTLARIVYGPTNLDIRGTSS
                     KTPLSIEGSPAAGDSGSGVFVQKGNDLWLVGITSYRYYSMYGGQAGFANLTHSANRDW
                     LNGIARSEKAVFGENVTPR"
     gene            complement(2000..3061)
                     /gene="ord"
                     /locus_tag="PKO06_05195"
     CDS             complement(2000..3061)
                     /gene="ord"
                     /locus_tag="PKO06_05195"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008825447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNV69072.1"
                     /translation="MIVRKEMVKVAIWGFGAMGGGMAEVLLRKKGVEIVGVCDIHPQR
                     AGKSIFEVLGVPRGSHKDVVIQSDISKVISKKSADVVLLCTDSFTAKSFDKMKLIVEL
                     GINVITTAEEMAYPKAKEPKLAAELDRLAKANGVSILGTGINPGLIMDLLVVLMTGAC
                     TDVDYVKAERVNSLSPFGPAVMEEQGVGITLDEFRKKSKEGHLAGHVGFNESVNMICD
                     AIGWKLDKPVEQTMDPIVSKVQRKTKYAEVKPGNVAGCTMKGFGYVNGKLAVEMIHPQ
                     QIEPELEGTNTGDYVIIKGTPNINLSNKPEVPGGIGTIAMCINMIPQIINAKPGLHTM
                     LDLPVPRAIMGDMREQIHE"
     gene            3436..4038
                     /locus_tag="PKO06_05200"
     CDS             3436..4038
                     /locus_tag="PKO06_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69073.1"
                     /translation="MTTIPTGTGNSIDQLLVAVTQGREEEVAELLRLGADPNGTDASG
                     VQTPLLNVVKTTHAGIARRLLAAGARVDKANVDGVTPLHWAAAKQKKEMARLMLEAGA
                     SVQARERDGWTPLHWAAYRGEKEMIEWFVDTGAAVNLPDKDGWTPLHLAAQQGHRPAV
                     DCLLDRGADPTLRDLDNRLPIDLARGRGHGDVVEVLQKVN"
     gene            4139..4399
                     /locus_tag="PKO06_05205"
     CDS             4139..4399
                     /locus_tag="PKO06_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV69074.1"
                     /translation="MIQITVIKSPSGCNTCEETGRIVAEATTKFPGVEIKIDLIVNGT
                     PEASAFGVITTPVVIINQKIYSMGKPVIAEKVESWIRKELGK"
     gene            4396..>4825
                     /locus_tag="PKO06_05210"
     CDS             4396..>4825
                     /locus_tag="PKO06_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69075.1"
                     /translation="MIGGEELVRELKQYQSICYYPSAGRDLSDIDYFASGCLPREERR
                     AGTGMKTVAATDPLPDLYVHTDVNFYQEYAAGSEFPVSECGIHGSCEIVGFRELPAIA
                     SPNRICNNFPHSGRVFEYKLRVWNSARIVTLLYCLCENEAF"
BASE COUNT         1044 a         1291 c         1411 g         1079 t
ORIGIN      
        1 ctttgccgaa cggcatcgcg gaaggtctgt tcttctgcgg ggatccaccg gtaagcagtg
       61 gtgacaagcg cctggacctg cccgatttcg gaacccgaag ccgtctcgag ccgctgcagg
      121 gcgttgaggg catgagctcg gctcaacgcc gtcgtgtcag tttgcgacaa gggaggtatc
      181 tcatcgatat acccgtcgac ataggcgagg cccatctcgg ccagcactcg attcaatgcg
      241 tagtcacggg tgagatcgag gtgaggatga atctgcatcc agccccacgc cagaccggag
      301 gcaagaaagc ctccaccacc ctttatgaaa cgattcagtg cctgttcatg atcccgatca
      361 ggaacaatgt tgtctccgac aagcagatca ataccagcga tttggtcttc ccaacctgga
      421 caactcagat gcctgacttc gaatcctgcc tgttcgagcg gtttggccag atcgtgggag
      481 aagatgaggc cgacgacggg tttggtcgat ctctgatgcg acggtctcga agcccaatga
      541 gcggcattga tgagcaatcg agtggtgtcg gcttcctgca gggctgcagt ctcgaagagt
      601 ccgccgtggc cgaaggcgac gatccgacct gaccccatgc gagcggcagc aacgacggga
      661 tgatagtgcc cgccggcttt gccaagcacc accggaaagg cgttttcgcg aatgcaagtc
      721 agcggcccgg gcactccagg tttggcgatg cgtttgactc cctcggtgag ttgtttgacg
      781 tcatccatcc acgctgacgg ttgagccgca acgaaggaga ggcgcatgat ggcgatcaga
      841 aagaagacca tcatgacttg ccgagattgc aaacgaacag gatttctgag acaacccggg
      901 aatacaccat ccatacagtt tcggttgttc atcatgtagt gcctgggaga tcaagtctag
      961 cgcggggtga cattctcgcc gaataccgct ttttcagaac gtgcgatgcc gttgagccag
     1021 tcacggttgg cggagtgggt gaggttggca aacccggcct gaccgccata catcgaatag
     1081 taccgatacg acgtgatacc gacgagccag agatcatttc ccttctgaac gaaaaccccg
     1141 cttccgctgt ctccggcagc gggagagcct tcaatggaaa gaggagtctt cgaagaagtc
     1201 ccgcgaatgt ccaggttggt tggcccgtat actattctcg ccagagtgtt tgcgccatca
     1261 gaaccgtcgt cgaagtccga tcggaacagc gccagttcgc tttcgtcgaa cagaaagtcg
     1321 atggtgttcg cataccctcg aaacgtgccc ataggcagag gttcattgtt gtcctttccc
     1381 gttcccgact ttccctgtcc tacgccaatg aagatttgtc cgagtcgaat ctgccccgaa
     1441 aagagacggg ccggaattgt gccgagtacg gattttgtca gtcgaagaac agcgagatcg
     1501 ccgcctccgc aatgtgttga gcgattatag cgcgggtgca gccatgcgta ttccgcctgg
     1561 taggcctttc cacggacctg gactgcgatc gacactcgtc cttcgttttt cccgtccggc
     1621 agaaatccca gaatcgggtg tccgcacgtc agaatgtgcc gcggcccaag caagacgccg
     1681 ctgaagtccc ctcgggcatc cggaattcgc accaagcctg catgcagccc ggcttgattg
     1741 gcgagatcaa gatacttgct ctcagagaca tcatgtcgaa tcatcacgcc gaaacatgcg
     1801 tgcgagagaa gcacgaagag caaagccaat ttcagcagtt tcatcagatc ctcctcatgt
     1861 ggggaattca aacaatcgat cgcattgggt tgtccaggtt atcatacctc gaaaatgtta
     1921 cgcatagcca gtctgccagc aaaagaaacg agggcggaac cgatgtgatt cgcgcccccg
     1981 taatgagatg cgactgacgt tattcgtgga tctgctcgcg catgtcaccc atgatcgcac
     2041 gtggaacggg caggtcgagc atggtgtgca agccaggctt cgcattgatg atctgcggaa
     2101 tcatgttgat gcacatggcg atggtgccaa tgccgccggg aacttcgggt ttgttcgaca
     2161 ggttgatgtt cggtgttccc ttgatgatca cgtaatcgcc ggtgttggtt ccctcgagtt
     2221 cgggctcaat ctgctgcggg tgaatcattt cgacggcgag tttgccgttt acgtatccga
     2281 agcctttcat ggtacagccg gcgacgtttc cgggtttcac ctcggcatac ttggtcttgc
     2341 gctgaacctt cgagacgatc ggatccatgg tctgttccac gggcttgtcg agcttccagc
     2401 cgatggcgtc gcagatcatg ttgaccgatt cattgaaacc gacatgcccg gccaggtgtc
     2461 cctctttcga cttcttcctg aattcatcga gagtaatgcc caccccctgc tcttccatga
     2521 ccgccggacc gaacggagag aggctgttca cgcgctcggc cttcacataa tcgacgtccg
     2581 tacaggctcc ggtcatgaga acgacgagta gatccatgat cagaccgggg ttgataccgg
     2641 tgcccagaat gctgaccccg ttggccttgg ccagtcggtc gagctcggca gccagcttgg
     2701 gttctttggc cttcggatac gccatttctt ccgcggtggt gatgacattg atgcccagtt
     2761 cgacgatgag cttcattttg tcgaacgact tggccgtaaa actgtcggtg cacagcagga
     2821 cgacatcagc cgattttttg ctgatgacct tgctgatgtc gctctgaatg acgacatcct
     2881 tgtggcttcc tcgcggaaca ccaaggactt cgaagatact ctttccggcg cgctgcggat
     2941 gaatgtcgca gacaccaacg atctcgacac ccttctttct cagcagcact tccgccatac
     3001 cgccgcccat ggctccaaat ccccagattg ctactttgac catctctttt ctcacaatca
     3061 ttcctcctgt tgatgaatgg tcgttcgacg agttctcaac gctcttcgcc tgcgcaggtt
     3121 gcgttgcacg aaacgtcgga cgaaagaaaa aacatacgag ggaagtctac aacaatcgcg
     3181 ttggatatca agtgaggtcg ttcggcaata gtggatacca aaacgttcat gcggcgactt
     3241 gttggttttc gagaaagaga tgctcgaaaa ccgctggtga gagaagcccg agcctggctt
     3301 gggttcgctg ccgagtgtag aagatttcga tgtctgcagt gatttctcgt cgggcctcct
     3361 cccgagtgga gtgtttcatg cggggtcagg gtagtggtat gctgactgat gaatgaacgt
     3421 ctacaggaga agtgaatgac cacgattccg acggggacgg gaaacagtat cgatcaactg
     3481 ttggtggcgg tgacccaggg ccgtgaggag gaagtcgccg agcttcttcg actcggtgct
     3541 gatccgaatg gcacggatgc cagcggtgtg cagacgccgc tgctcaatgt cgtcaagacg
     3601 acgcatgcgg gaattgcgcg tcggttgctc gctgccggag cccgagtgga caaggcaaat
     3661 gttgacggag tgactccgct gcactgggcc gctgcgaaac agaagaagga aatggctcga
     3721 ttgatgctcg aagcgggcgc ttcggttcaa gcccgcgaac gagacggctg gaccccgttg
     3781 cactgggctg cctatcgggg cgagaaggaa atgatcgaat ggtttgtgga tacgggagca
     3841 gccgtgaatc tgcctgacaa agacggttgg accccgctgc accttgccgc gcagcagggc
     3901 catcggccag ctgtcgattg tctgctggac cgtggtgcag acccgacact tcgtgacctc
     3961 gacaatcgtc tgccgatcga tcttgctcgc gggcgaggtc atggtgacgt ggtcgaagtg
     4021 ctgcagaaag tgaactgagg cggtggtgat gtggttgacg gtcgtgagtc gagggtgacg
     4081 tgttcatggc atcgatgcgg gaagcgacct ttctgagaga caatccggga gcaattcaat
     4141 gattcagatc acggttatca agagtccgag tggctgcaac acctgcgaag aaaccggtcg
     4201 tatcgtcgcc gaggcgacga cgaagtttcc cggggtcgag atcaagatcg atctcatcgt
     4261 caatggcacg ccagaggcca gtgctttcgg ggtgatcacc acacctgtcg tgatcatcaa
     4321 tcagaagatc tactcgatgg gcaagccggt gattgccgag aaagtggaat cctggattcg
     4381 caaggagctg gggaagtgat cggcggagag gaacttgtcc gcgagctgaa acagtaccag
     4441 agcatttgtt actatccctc ggctggaaga gacctgagcg acatcgacta ctttgcgtct
     4501 ggttgtctgc ctcgagaaga gcgtcgggct ggcactggaa tgaagaccgt tgctgctacc
     4561 gacccgttgc cggacttgta cgttcacacc gatgtgaact tctatcagga gtatgccgcc
     4621 gggtcagagt tccccgtgag cgagtgcggg attcacggct cgtgcgagat cgtgggattt
     4681 cgtgaactcc ccgccatcgc ttctcccaat cgtatctgta acaactttcc gcatagcggc
     4741 agggtgtttg agtacaagtt gcgcgtctgg aactccgcgc gaattgtcac tttgctgtac
     4801 tgcctgtgtg aaaatgaggc gtttg
//
LOCUS       DAORHD010000182         4823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18491_length_4823_cov_3.591023, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000182 DAORHD010000000
VERSION     DAORHD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18491_length_4823_cov_3.591023"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKO06_05215"
     CDS             complement(<1..277)
                     /locus_tag="PKO06_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV69076.1"
                     /translation="MCGLIYQMVWFQQLKLVFGTSTAASAAVTAIFMFGLGWGSLKLG
                     HRADQQTRPLVLYGRLEVGIALCAALSPLLMSVVRSVYLATGGTMTLG"
     gene            complement(564..1241)
                     /locus_tag="PKO06_05220"
     CDS             complement(564..1241)
                     /locus_tag="PKO06_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69077.1"
                     /translation="MHPLIFSFRLLIKLTVITLLCTGMSSWSLSAASKGTPSTQTVDE
                     DFQDEGNTGGASPDEEETAALNEELEPDQVVLDDTTMDQPLVPQPTPENPSQTPTSVQ
                     PTDKIEQSLQQPLIAQESKTSGNWKAGDRCEATGVLKYHDSRVMSVESFKGHEFTINR
                     VPVKPTAKVSIETLKKHVGKKVHVSGTWDAGTIFTPNPKEPSAYPTDAVGQATGDGIL
                     VDLIELK"
     gene            complement(1294..1890)
                     /locus_tag="PKO06_05225"
     CDS             complement(1294..1890)
                     /locus_tag="PKO06_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69078.1"
                     /translation="MRNTRLLVLMIFGTLFLTASLTAQVVFKPLADDEVKILDGLSGL
                     LSRTPQAIEVEQVRKAAQWPSPIVRCLATVLLKKWGDTSADRLLLEHFSVNDYVERSK
                     GVQHLITPDQMIEAVKAAEVSCKALKANGAIMLVLYNHFRNKNQWMPDPAGTGQNIEM
                     ARFFRTAYFAGVFKGTSIDAVKTSMMIDIATRKASGVK"
     gene            complement(2048..2410)
                     /locus_tag="PKO06_05230"
     CDS             complement(2048..2410)
                     /locus_tag="PKO06_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69079.1"
                     /translation="MAWWRNFWYTDENFYALVAVTISYVATMPALLTFGFAHRYKSDP
                     SHRLSFGMLFLLHTLNHLGLFFFIKLDHKLEILRYFGVLMTPFAIFILSYYCLCLLGI
                     GYIQVTRTIHDRSPSPDP"
     gene            complement(2500..3618)
                     /gene="phnD"
                     /locus_tag="PKO06_05235"
     CDS             complement(2500..3618)
                     /gene="phnD"
                     /locus_tag="PKO06_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV69080.1"
                     /translation="MTLAFRSFPASRFMLVSAFLCALLMAASPALEASKDAPTLNELM
                     ASGELEKEISDDPPARTAIVPDDEADPIIPSEAPESLSDEDQAPVAPQPAEPTIKLTP
                     VPRVPPAEKTVLIGRVPFMNTENMLLAYRGLIAFLKQEMGVKDVRIVTAKDYAGVQNA
                     LERGSIDFAWLGPMAYAIASQKIPMYPLAQANRRTGSTYRGVFITRVDSKILGIEEIK
                     GRVVGFVDPESASGYLYPLYFLQRSKINPHEVCKKVEFLGKHDAIIPAVLAGKIDVGV
                     CLEDTLQAVKDKRILDRLLILGKTYEVPSDIVACRKDCDPKLRDAFQRAMLKTRSLKQ
                     KADPATGLPPVLEFMPVNDADLDPVRGVLGAIDSIRQH"
     gene            complement(3717..4472)
                     /locus_tag="PKO06_05240"
     CDS             complement(3717..4472)
                     /locus_tag="PKO06_05240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69081.1"
                     /translation="MTDIGVGSMIYRGDIYDKMNPFISDLSFFEKWSRQSRGDVLELC
                     CGTGRLTIPLHQAGIPISGLDITPTMLDAARAKCAKAGLQIPLHQGDIRTFDLAKKFS
                     LIFIPFNSLQHTYSIEDLEAVFSRVKAHLNPDGLFIFDVFNPSIHMMVDREREIGEAF
                     RFPLDDGRELVVRERCHYDAGTQINQVTWFFRIDQEEEHPEQLHMRCFFPLELTMALK
                     YNGFSVVHRFGAFDESPFTSASPKQIFVCRANA"
     gene            4628..4807
                     /locus_tag="PKO06_05245"
     CDS             4628..4807
                     /locus_tag="PKO06_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69082.1"
                     /translation="MPFCRHGFIVLLIIWFSGVGLAMAQDFQLESSVDRTEIAAGESV
                     ILTLTVTQSLQGGSP"
BASE COUNT         1100 a         1275 c         1406 g         1042 t
ORIGIN      
        1 ggcccaacgt catcgtgccg ccggttgcga gatacacgga ccgaacgacg ctcatcagca
       61 gaggagaaag tgcggcgcac agggcgatac cgacttcgag gcgaccgtac aacacgaggg
      121 ggcgggtttg ctgatcggct cgatggccga gtttcagact gccccagccg aggccgaaca
      181 tgaaaatcgc cgtcacggct gccgaggccg cggtcgaggt gccgaagacc agtttcagct
      241 gctgaaacca gaccatttga taaatgaggc cgcacatgcc gcttcccaaa aggaaaaggg
      301 ccgccggaag actggacgca ctcatgacgc actccattgt cgtggaaatt gagaggctct
      361 gctgatcgtg ttcggaaaaa tcgcactctc gcaccgtcgc ccgacagagt agtacatttt
      421 gcccggttcc acaataggcg gtgcacgacc tctccgcgat gccgttcgga actgaaagcg
      481 agaaaaggtt gtcgaagaag gaaacaccct cgacggtcgg tgagacgacg gtcgagggtg
      541 aaaccgagag cggtgtctgg tggttacttc agttcgatca ggtcgacgag aatgccatca
      601 cctgtcgctt gtccgaccgc atcggtcggg tacgccgatg gttctttcgg attcggcgtg
      661 aaaattgtgc ctgcgtccca ggtgcctgaa acgtgaacct tctttccgac atgctttttc
      721 agggtttcga ttgatacctt ggctgttggc ttgaccggta cgcgattgat ggtgaattcg
      781 tgccccttga atgattcgac gctcatgacc cgactgtcgt gatacttcaa aaccccggtg
      841 gcctcgcaac gatcgccggc tttccagttc ccggaggtct tgctctcctg cgcaatgaga
      901 ggttgctgaa gcgactgctc gatcttgtcg gtcggttgca cactggtcgg agtctgggag
      961 gggttttccg gggtgggctg ggggacaaga ggctgatcca tggttgtatc gtcaagcacg
     1021 acctgatctg gttccagttc ttcattcagg gcggccgttt cttcttcgtc cggcgaggct
     1081 cctcctgtgt ttccttcatc ttgaaagtcc tcatcaactg tctgagtcga aggggttccc
     1141 ttggaagcgg cagatagtga ccacgaagac ataccggtac agagaagggt aatcaccgta
     1201 agcttgatca gcaggcgaaa cgagaagatg aggggatgca tgggttggac tcctaagttg
     1261 aattgaaagg tgttcgttct cgaggaaacg acgttacttg actccagagg ctttgcgcgt
     1321 cgcaatgtcg atcatcatcg aggtcttgac cgcatcaatg ctggtgccct tgaacacacc
     1381 tgcgaaatac gccgttcgaa aaaaacgggc catctcgatg ttctgaccgg tgccagcggg
     1441 gtcgggcatc cactggtttt tgttccgaaa gtgattgtac agaaccagca tgatggcacc
     1501 attcgctttc agtgctttgc aggaaacctc ggccgctttg accgcctcaa tcatctggtc
     1561 gggagtaatg agatgctgca cccccttgct gcgctcaacg tagtcgttga ccgaaaagtg
     1621 ctcgagcaac agacgatcgg cggacgtgtc gccccacttc ttcagaagca cggtcgcgag
     1681 acagcgcacg attggtgatg gccactgcgc agccttgcgt acctgttcca cttcgatcgc
     1741 ctgtggcgtg cgcgacaaca agcctgacag accgtcgaga atcttgactt catcatccgc
     1801 aagtggtttg aacacgacct gcgccgtcaa ggacgcggtc aggaaaagtg tgccgaagat
     1861 catcaacacg agcagtctgg tgtttctcat ctcacaggtt cctcccggga tctgatctga
     1921 agataaacga ttcgctcctt ctcattacag ccgattatcg catggttgac acccgtgggc
     1981 aagatcattg gctgggaagc cgggaaggca attccctgct gccaagccag tggcctcggt
     2041 gtcaccttta cgggtcaggc gaaggcgagc gatcgtgaat ggtacgggtg acctgaatgt
     2101 agccgatgcc aagcaggcac agacagtagt aggataggat gaagatggca aacggcgtca
     2161 tcagaacgcc gaaatacctg agaatctcaa gtttgtgatc gagtttgatg aagaagaaca
     2221 agcccaggtg gttcagggtg tgcagcagga agagcatgcc gaatgacaag cggtgagagg
     2281 gatcgctttt gtaccggtga gcgaaaccaa aggtcagcag tgcgggcatc gttgcgacat
     2341 aggagatggt cacggcaacc agagcgtaaa aattctcgtc cgtgtaccag aagtttctcc
     2401 accaggccat caagtctatc ataagcttgt cacaagcaat actgaccgat agacaactgg
     2461 gacaaaacga caaacactct catgaactgg gagaggcgct cagtgctggc ggatgctgtc
     2521 gattgctccc aggactccgc gcaccgggtc gagatcagcg tcgttgaccg gcatgaattc
     2581 gagcacaggc ggcagtccgg tggccggatc agctttctgc ttcaacgatc gggttttcag
     2641 cattgcccgc tggaaagcgt cacgcaattt gggatcgcag tccttgcgac aggcgacgat
     2701 gtcggaggga acctcatagg tcttgccgag aatcagcagt cggtcgagaa tccgtttgtc
     2761 cttcaccgcc tgcagggtgt cttcaagaca gaccccgaca tcgattttac cggccagcac
     2821 tgccggaatg atggcatcgt gtttgcccag aaactcgacc ttcttgcaga cctcatgcgg
     2881 attgatcttc gaacgctgca gaaaatacaa cggatacaga tacccgctgg ctgactcggg
     2941 atcgacaaag ccgacgaccc gacccttgat ctcttcgata ccgagaatct ttgaatcgac
     3001 ccgcgtaatg aataccccgc gatacgtgct tccggtgcgg cgatttgcct gagccaacgg
     3061 gtacatggga atcttttgcg aggcgatggc atacgccatg ggtccgagcc aggcaaagtc
     3121 gatgctgcct cgttcaagag cgttctgtac gccggcatag tccttggcgg tcacaatgcg
     3181 cacgtccttc acgcccattt cctgtttcag aaacgcgatc agaccacgat aagccagcag
     3241 catgttttcg gtattcatga aggggacccg accgatcaac acggtcttct cagccggcgg
     3301 cacgcgtggc accggtgtga gtttgatcgt gggttccgcc ggttgagggg cgaccggagc
     3361 ctgatcttca tcgctcagtg actcgggggc ctcggacgga atgatcggat cagcttcgtc
     3421 gtcaggtacg atggccgtcc gggcaggcgg gtcatcagag atttccttct cgagttcacc
     3481 gctcgccatc agttcgttga gagtaggagc gtctttcgag gcttcgaggg caggcgacgc
     3541 cgccatcagc agcgcgcaga gaaacgcaga aacgagcatg aagcgggagg cgggaaaaga
     3601 acgaaaggcg agggtcatgg aagctctcct gataacacaa tgcgatgagt tgacaccgtc
     3661 agatctgacg tgttgacaat actcgttctc accattgccc ggcaacccgt tttcgctcag
     3721 gcattggcgc gacacacgaa gatctgcttc ggtgaggccg aggtgaacgg actctcgtcg
     3781 aaagccccaa accgatgcac gaccgagaag ccgttgtatt tcagggccat ggtgagttca
     3841 agcggaaaga aacatcgcat gtgcagttgc tccggatgtt cctcttcctg gtcgatgcga
     3901 aagaaccagg tgacctgatt gatctgggta ccggcgtcgt agtgacaccg ttcacggacc
     3961 accagttccc gcccgtcgtc gagtggaaaa cggaaggctt cgccgatctc gcgctcacga
     4021 tcaaccatca tgtgaatgct ggggttgaac acatcgaaga tgaacagtcc gtctgggttc
     4081 agatgcgctt tgacccggct gaagactgcc tcgaggtctt cgatcgaata ggtgtgctgc
     4141 aaggaattga acgggatgaa gatcaggctg aacttcttcg ccagatcaaa ggtgcgaatg
     4201 tcgccttggt gcagcggaat ctgaagcccg gccttggcgc atttagctcg ggcggcgtcg
     4261 agcatggtcg gagtaatgtc gaggcctgag attggaattc cggcctggtg caaaggaatc
     4321 gtgagtcggc cggtaccaca gcacaattcg agcacgtcgc ctcgagactg tctgctccac
     4381 ttctcgaaga acgacaggtc agaaatgaag gggttcatct tgtcgtagat atccccacga
     4441 tagatcatgg atcccacgcc gatgtcggtc atatttacct ctcctgatac agcttcctgc
     4501 tcacatgaag aagatagcac atcagtaaaa aattccggga acctttttga tgacgattgg
     4561 tctaagcagg tgtatacgaa gagggggcgt tctgcccttg aatcgatgga acagggaggc
     4621 acggcacatg ccattctgcc ggcatggttt catagttctg ctgatcatct ggttttccgg
     4681 ggtcgggctg gccatggctc aggattttca gctcgaatcc tcggtcgatc gtacggaaat
     4741 cgccgcaggg gaaagtgtga ttctgacact gaccgtaacg cagagcctgc aaggcggatc
     4801 cccatgaatc accgacccgc aca
//
LOCUS       DAORHD010000183         4812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18548_length_4812_cov_3.523019, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000183 DAORHD010000000
VERSION     DAORHD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18548_length_4812_cov_3.523019"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKO06_05250"
     CDS             <1..517
                     /locus_tag="PKO06_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV69083.1"
                     /translation="IMVKKEAFANPITGWFLRKVCAFPIDRQNPDAATLRYSLKVLQQ
                     GMVLGLFPEGTRNRKGLIRPFKPGAIRLALKQKAPIVPAFLANTHRIMPDGTWVPRPV
                     PVSMKFGEPLDTKKMLEEGKTEEDMLKILYERVCALGRELTGCDVQDHSTEEPEEPTQ
                     AQTQNPVAATE"
     gene            569..2905
                     /locus_tag="PKO06_05255"
     CDS             569..2905
                     /locus_tag="PKO06_05255"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV69084.1"
                     /translation="MIDFIQAILTRRLKDTELKHFLTDLEHADRRKSLETIRAFFHQV
                     ASLLEVSHQLLDNLLMLVLGVTTKLIGAERGTLFLIDQETGELFSRVAQGDEVHEIRI
                     PLNTGIAGAIVTQGRPVIIPNAYADPRFNAEIDKQTGFRTRDILGAPIRISPQHETIG
                     VLELLNRSQGTFSADDLTLLETITSQAAIALRNARDLECNQQARESEIRLQEVTTAIS
                     SELKLKPLLEKIMQAVTLILNADRATLFLHDEKTNELWSQVALGLQDQEIRFPAHLGI
                     AGRVFTKGETINIPEAYADERFNKEIDRKTGYVTRNILCMPVINRAGKAIGVTQVLNK
                     KSGVFTRLDEKKLRTFSAQAAIAIENAKLFDEVLNMKNYSESILESLSNGVITVNAIR
                     RIEKCNAVALKSFGVTPEQITGKFADIYFTGDNHWIVDSLRRVVATQEPDNFIDAEIV
                     NAQGKHISVNMAIAPLHDSQGKPMGGLLVFEDLTTEKRMKSTLARYMTKEVADQLLTT
                     DDAMLGGKMQELTVLFSDIRHFTTISEKLGPQATVSLLNEYFSSMVDIVFHHGGILDK
                     FIGDAMLAVFGAPFSSGEDPDRAVTTAIEMIKALKDFNRDRATKGLEVIEIGVGINTD
                     QVLVGNIGSLKRMDYTVIGDGVNLASRLEGANKYFGTNILVSENTMKQLKYRHLHREV
                     DLLRVKGKMQPATIFEILDHHDRSTFPHLEETIGWYQSGLQDYRKQRWTDAEKKFLQA
                     LALHPGDQLSRVYLDRCRHFVATPPPPDWDGVWVMEIK"
     gene            complement(2914..4362)
                     /locus_tag="PKO06_05260"
     CDS             complement(2914..4362)
                     /locus_tag="PKO06_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV69085.1"
                     /translation="MSVLLLLTVASLGWAEAPRVEKEWSFLVFINADNNLDEYGVLDL
                     EEMARVGSNDRVNIVTLIDRLQAPATMNYIEKGKITPISEPVELDMGDYRELVNFVGV
                     AVERYPARNYCLIVWNHGAGWKQKGAALKGISYDDQSGNHITTEQLGIALREVNGLLG
                     RPIDLFCMDACLMQMLEVAWEIRRSCRYLVASEETIPGDGFPYEQVLKALKPGIGPRE
                     FGAEIVREYKLCYSQDVSDGGVWDVTNRPRRERVVKKTTLSLLDCARLDDLKTAVDGF
                     AETLMAVDQAQNVAAALKETHSFTYSTNVDLWHLVDLVMAKVPSSMVKNAGQKVKDEF
                     KNVVLANGVTGDNDQTWPEPDTDDLRRGRFPIDAHGLAIYFPDKKASFAQNYLDLTYA
                     RESRWDEFLHDHYLKLAAREALDGLSRGTVDQLRQYVADARPADRETHRTLLRQANFT
                     LFCEGLLSPAVRYQATGLLKQLAATMASAAEN"
     gene            4607..>4812
                     /locus_tag="PKO06_05265"
     CDS             4607..>4812
                     /locus_tag="PKO06_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69086.1"
                     /translation="MQKRVVVALVLVFQILGLVAVWAAETKLTADQQNLLEKLSLMRE
                     VWQRKITLEYLADKKERVSVQIFGT"
BASE COUNT         1055 a         1457 c         1348 g          952 t
ORIGIN      
        1 catcatggtg aagaaggaag cctttgccaa cccgatcacc ggctggtttc tgcgcaaggt
       61 ctgtgcgttt ccgatcgacc gccagaaccc ggatgcagcg accctgcgct actcgctgaa
      121 ggtgttgcag cagggcatgg tcctgggctt gttcccggaa ggcacccgca accgcaaggg
      181 cctcattcgc cccttcaaac cgggcgcgat tcgtctggca ctcaagcaga aggccccgat
      241 cgtgccagcc tttctggcga acacgcatcg catcatgccg gatggaacct gggtgcctcg
      301 accggtgccg gtttcgatga agttcggcga gcctctcgac acgaagaaga tgcttgaaga
      361 gggaaagacc gaagaggaca tgctgaagat tctgtatgag cgcgtctgcg ctctcggtcg
      421 tgagctgacc ggttgcgacg tgcaggacca ctcgaccgaa gaaccggaag aaccgacgca
      481 ggctcagacc cagaaccctg tcgccgccac tgaataggag aatgacacgg tcgatgtccc
      541 aggtattgca cgggaacaag agcttctgat gattgacttc attcaggcga ttctcacacg
      601 tcggctcaaa gacacagaac tgaagcactt tctcaccgat ctcgaacatg ccgaccgccg
      661 caagtcgctg gaaaccattc gcgcgttctt ccatcaggtc gcgtcgctgc tcgaagtctc
      721 gcatcaactg ctcgacaatc tgctgatgct ggtgctgggg gtcacaacca agctgatcgg
      781 tgccgagcgc ggcactctgt ttctgatcga ccaggagacg ggcgaactgt tttcccgcgt
      841 ggctcagggc gatgaagtcc acgagattcg cattccgctg aataccggca tcgccggtgc
      901 catcgtgacc caggggcgac cggtcatcat tccgaacgcc tatgccgacc cccgtttcaa
      961 tgccgagatc gacaagcaga cagggtttcg cacccgcgat attctcggcg ctcccatccg
     1021 gatcagccca cagcatgaaa ccatcggggt gctcgaactg ctgaaccgct cgcagggcac
     1081 gttcagcgct gacgatctga cgctgctcga gacgatcacg tcgcaggcag ccatcgccct
     1141 gcgcaacgcc cgcgatctcg agtgtaacca gcaggcccgc gaaagcgaaa ttcgcctgca
     1201 ggaagtcacg accgccattt cctcggagct gaagctgaaa ccgctgctcg aaaagatcat
     1261 gcaggcggtg acgctgattc tcaatgccga ccgggcgacg ctctttctgc acgatgagaa
     1321 gaccaacgaa ctctggtcgc aggtggcgct cggcctgcag gaccaggaaa tccggtttcc
     1381 cgcgcatctg ggcattgccg gtcgcgtctt caccaagggc gagacgatca atattcccga
     1441 agcctacgcg gatgagcgtt ttaacaaaga gatcgaccgc aagaccggct atgtgacccg
     1501 caacattctc tgcatgccgg tgatcaaccg cgccggcaag gcgatcggcg tcacgcaggt
     1561 gctgaacaag aaaagcggcg tcttcacccg actcgacgaa aagaagctgc gcacgttttc
     1621 ggcgcaggcc gccatcgcca tcgaaaatgc caagctgttc gacgaagtgc tgaacatgaa
     1681 aaattacagc gagagcattc tcgaaagtct cagcaacggc gtcatcaccg tgaatgcgat
     1741 tcgccgcatc gagaagtgca acgcggtcgc gctgaagagc ttcggcgtca cccccgagca
     1801 gatcaccggc aagttcgccg acatctactt tactggcgac aaccactgga tcgtcgacag
     1861 cctgcgccgt gtcgtcgcca cgcaagagcc cgacaacttc atcgatgccg agatcgtgaa
     1921 tgcgcaaggc aagcacatct cggtgaacat ggccatcgcc ccgctgcacg actcacaggg
     1981 gaagccgatg ggcgggttgc tggtcttcga agacctgacc acggaaaagc gcatgaagag
     2041 cacgctcgcg cgctacatga ccaaagaggt cgccgatcag cttttgacga cggatgacgc
     2101 gatgctgggc ggcaagatgc aggaactgac cgtgctgttc agcgacattc gccatttcac
     2161 caccatttcg gaaaagctcg gcccgcaggc caccgtgagt ctgctgaatg agtacttctc
     2221 gagtatggtc gacatcgtgt ttcatcacgg agggatactg gacaaattca tcggtgatgc
     2281 gatgctggcc gtcttcggtg cgccgttttc ctcaggcgaa gaccctgacc gcgccgtgac
     2341 caccgcgatt gaaatgatca aggctctgaa agattttaac cgtgaccggg ccaccaaagg
     2401 gctcgaagtg atcgagatcg gcgtcggcat caacactgac caggtgctgg tcggcaacat
     2461 cggttcgctc aagcgcatgg attacacggt catcggtgac ggggtcaatc tcgcctcgcg
     2521 actcgaaggt gccaacaagt atttcggcac caacattctt gtcagcgaga acacgatgaa
     2581 acagttgaaa taccggcacc tgcaccgtga agtcgacctg ctgcgggtca aaggaaagat
     2641 gcaacccgcg acgatcttcg agattctcga ccatcacgac cgttcgacgt ttccgcatct
     2701 cgaggaaacc atcggttggt accagtcagg tctgcaggat taccgcaagc agcgctggac
     2761 agacgccgag aagaagtttc tgcaagcgct cgccctgcac ccgggcgatc agctctcgag
     2821 ggtctacctc gaccgctgcc gccactttgt cgccacccct cccccacccg attgggacgg
     2881 cgtctgggtg atggagatca agtaaacggc tgatcagttt tcagctgcgg aagccatggt
     2941 cgcggcaagc tgtttgagca gccccgtcgc ttgataccgc acggccggag acagcaggcc
     3001 ttcacagaac agcgtgaagt tcgcctgccg caacagcgtg cggtgagtct cacggtcggc
     3061 cgggcgggca tcggccacat actgccgcaa ctggtcgacc gttccacggc tcaacccgtc
     3121 gagggcttcg cgcgcggcca gtttcaggta gtggtcgtgc aggaattcat cccagcgact
     3181 ctcgcgcgcg tacgtcagat cgaggtagtt ctgcgcaaac gacgcttttt tgtcgggaaa
     3241 gtagatcgcc agaccgtggg catcgatggg aaaccgcccg cgacgcagat catcggtatc
     3301 aggttcaggc caggtctggt cgttgtcgcc cgtcacgcca ttcgccagaa cgacattctt
     3361 aaactcgtct ttcaccttct gacctgcgtt tttcaccata ctcgatggga ctttcgccat
     3421 caccagatcg accagatgcc agaggtcgac gttggtcgag taggtaaaac tgtgtgtctc
     3481 tttcagggca gccgcaacat tctgtgcctg gtcaaccgcc atcagcgttt cagcaaagcc
     3541 atcaacagcg gttttcaggt catcgagccg cgcgcaatcc agcagcgaca aggtggtctt
     3601 tttcacgacc cgctcgcgtc gcggtcgatt tgtcacatcc catacgccgc cgtcactgac
     3661 atcctgcgag tagcacaact tgtactcacg cacgatttca gctccgaatt cacgcggacc
     3721 gattcccggc ttgagtgcct tcagcacctg ttcatacgga aacccgtctc ctggaatggt
     3781 ttcttctgat gccaccagat accggcagct gcggcgaatc tcccaggcga cctcgagcat
     3841 ctgcatcaga caggcatcca tgcagaacag atcgatcggg cgaccgagca gcccgttcac
     3901 ctctcgcagg gcgattccca gctgttcggt ggtgatatgg ttcccgctct ggtcgtcgta
     3961 ggagatgcct ttcagagccg cgcctttctg tttccagccg gcaccgtgat tccagacgat
     4021 gagacagtag ttccgggcgg gatatcgctc aacggcaacc ccgacaaaat tcaccagctc
     4081 gcgatagtcg cccatgtcga gttcgaccgg ttcgctgatc ggggtaatct ttcccttctc
     4141 gatgtaattc atggtcgcgg gtgcctgcag gcgatcgatg agcgtgacga tgttgacgcg
     4201 atcgttcgag ccgacccggg ccatttcttc gaggtcgagc accccgtatt catcgaggtt
     4261 gttatcggca ttgatgaaga ccagaaagct ccattccttc tcgacccgag gggcttcggc
     4321 ccaccccaga ctcgcgaccg tcaacagcaa cagcacactc atcaagcgct tcatcttttc
     4381 accctcactt ttttacttct gctcatgata ccacgacgaa caccctccca tccgtatcgg
     4441 gagatcgggg aacgggcaca tgccaacttt cacctgatcc aaagggcgtt tcggctcacc
     4501 tcccagtgat gaacggcgga gtgagccggg agggtttctc gacagtggcc gtgaaagcca
     4561 gaatcgggta tactgtgcgc atacatttca gccacaggag agacgtatgc agaaacgagt
     4621 agtagtggca ctggttcttg tctttcagat tctcgggctc gtggccgtct gggcagccga
     4681 aacgaagctc accgccgacc agcagaacct gctggaaaag ctgtcgttga tgcgcgaggt
     4741 ctggcaacgc aagatcacgc tcgagtatct ggcggacaag aaggagcggg tgtcggtcca
     4801 gatcttcggc ac
//
LOCUS       DAORHD010000184         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18562_length_4809_cov_3.389987, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000184 DAORHD010000000
VERSION     DAORHD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18562_length_4809_cov_3.389987"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="PKO06_05270"
     CDS             complement(<1..1017)
                     /locus_tag="PKO06_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNV69087.1"
                     /translation="MLAKLLSGTIAGYEPLLVEVEVDIAGGLPSFTMVGLPDASVNEA
                     RERVRAALKNAGYELPARRITVNLAPADVRKEGAGLDVPLAVALLTALGEIPAERVEG
                     LLFLGETSLAGELRHARGVLPLSLLAKEKNLKGVVVPRTNVREAAAIPSLPVYGFDKL
                     EDLIRSLKEAESLPPAYVETSGNTPLPPAFQHVDLAEIKGQPFARRALEISAAGAHNL
                     LMAGPPGAGKTLLARTFPSLLPPLSFSESLEVSRIYSIKGLLPPECGLVQHRPFRDPH
                     HTISDVALIGGGRIPAPGEVSLAHHGVLFLDEIPEFRKSVLEVLRQPLSEGNVSISRA
                     AQTVT"
     gene            1139..2515
                     /locus_tag="PKO06_05275"
     CDS             1139..2515
                     /locus_tag="PKO06_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69088.1"
                     /translation="MDQHLLQSLTDKAALEEADKEDLLQIIEDTIILGRAELADIQLQ
                     VMKDRFPNEPEFLYAQARVLKEQGQVQEAYNLFKRLYFEQPIFMSERRDYETTRDQLL
                     KDKLEKAKAQWNLVLSLAQRLASREAPSPKQPPAEDNIQAIKDQIHAEIDKLIDMYND
                     VLKDEPQHLQALKGLVYCYTDRSETAQASAHQERLKLAHSYWNDLGSRRAAVCLNEAR
                     RVLDEEKKDLAIDLINRGLDTAPSHSGLLVQKAEILRGLQKFKEAAQCIEVLLRESPN
                     DSEGLKMKKNLQNDRLEESIKRGWLMIKEMESGTQTESQTRTTLKEAMDLFFTCLDID
                     ANSLRALEGIYRCQLFSNNPLKAKKTLERIKEIDSTYRVGTVGSTQPPAADQADQCFV
                     ATRIFGPRHPTTCRLREFRDTHLRHTISGRVFIRWYKRIGPRLAALPRRSPTVRLLAR
                     VLKTILPH"
     gene            complement(2605..3489)
                     /locus_tag="PKO06_05280"
     CDS             complement(2605..3489)
                     /locus_tag="PKO06_05280"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNV69089.1"
                     /translation="MNFSELEKKLQANRVPLIICMPEDQEAILAAQEAMEMGYVDCVF
                     VGNRARITELVKQVAKNFEPEIVDAEGEEDAAAKSVALIRSGRGKALMKGTIATPKLL
                     KAVLNSENGIKAGDVLSHVLVYEWEGRFKYLTDGGMVPHPTLAEKQAILANAVDLARR
                     LGDAKPKVAVLSALETVNLKMPSTVDAAVLAKMGERKQFGSCQVEGPLALDNAISVES
                     AHHKGLHGDVVGQADILVCPDIDTGNVLGKSLMYFANVPAGGLIVGAKVPIILLSRAD
                     SKQTRLNSIKLALASGLN"
     gene            complement(3502..4572)
                     /gene="buk"
                     /locus_tag="PKO06_05285"
     CDS             complement(3502..4572)
                     /gene="buk"
                     /locus_tag="PKO06_05285"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNV69090.1"
                     /translation="MKQPLLLVINPGSTSTKVALFDGTQSRAEQEITHPGSELSKFSS
                     NLEQLDFRLGEVRRALETWKIQPRDLQAVIGRGGPLKPMEGGVYRLGDALLNDLRSGK
                     LVDHASILGGLLAYRIAGEAGIPAFIADPVSVDEFDDLARISGHPDLPRISLAHALNC
                     KAVIREVARDQKRNPEDLNLIVAHLGGGISIVAHRQGRQIDVNNANDSGPFSPERAGT
                     LPLTGLLKLACSGKHTLPELRKMLIGKGGLIAHLGTADVREVQKRIDSGDQKARLVLE
                     AMAYTIAKEIGAMATVLKGKVDTIILTGGVAYSKTVTDWITERVQFIAPVVIKPGQNE
                     LKALAEHAVTALQNPSMVKDYR"
     gene            complement(4600..>4809)
                     /locus_tag="PKO06_05290"
     CDS             complement(4600..>4809)
                     /locus_tag="PKO06_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69091.1"
                     /translation="YPNKRVRLHFVRCTPVPGNENLTRKWPAEQAAWFRPEETLPPLC
                     PADQLAWEQMPWSRLLPRATSSVRE"
BASE COUNT         1020 a         1420 c         1393 g          976 t
ORIGIN      
        1 ggtgacagtc tgggcggcgc ggctgatcga gacgtttccc tcgctgagcg gctgccgcag
       61 aacttcgagc acggacttgc ggaactccgg aatctcatcg agaaacagta cgccgtggtg
      121 tgccagtgag acttcgccgg gggcggggat acgcccgccg ccgatcagcg cgacatcaga
      181 gatggtgtga tgcgggtcac ggaatggccg atgctggacc aacccgcatt cgggcggcag
      241 cagccccttg atcgagtaga ttcggctgac ttcgagcgat tcggaaaagg acaggggtgg
      301 gagcagcgag gggaacgtcc gcgccagcag agtcttacca gctcccggcg gcccggccat
      361 cagcaggttg tgggctccgg cggcactgat ctcgagcgcg cgacgggcga acggctggcc
      421 cttgatttcc gcgagatcga catgctgaaa cgccggcggc agtggggtat tgcctgacgt
      481 ttcaacgtag gctggaggca gactctctgc ctctttcagt gatctgatca aatcttcgag
      541 tttgtcgaac ccgtacacag gcaacgaagg aatggcggcg gcttcacgca cgttcgtgcg
      601 cggaaccacc acgcccttga gattcttctc ttttgccaac agcgacaaag gcagcacgcc
      661 gcgggcgtgg cgcagttctc cggcaagtga ggtttccccg agaaacagca acccttcgac
      721 gcgttcggcg ggaatttcac ccagggcggt cagcagagcc acggcgagcg gaacatcgag
      781 accggcacct tccttgcgaa cgtcggcggg cgcgagattc accgtgattc gacgagccgg
      841 aagttcatac cccgcgttct tcaacgcggc ccgcacccgt tcccgggctt cattgaccga
      901 ggcatcaggc aaccccacca tggtgaacga gggaagacca ccggcaatgt cgacctcgac
      961 ttcgacgagc aaaggctcgt atccggctat ggttcctgag agaagttttg ccagcatgta
     1021 cgtattttac tcgattgagt cgcgaattca agatcccgta acagccggcg aatatgatgc
     1081 tgaaatggtt gcatgaaaaa gccggttgca tatatagtag acagggaatc acgattacat
     1141 ggaccagcat ctccttcaaa gcctgaccga taaagcggcc ctggaagaag ccgacaaaga
     1201 ggatctgctt cagatcattg aagatacgat cattctcggc cgggccgagc ttgccgatat
     1261 tcagttgcag gtcatgaaag accgttttcc caacgagccc gagttcttgt atgcgcaggc
     1321 acgcgtgctg aaagaacagg ggcaggttca ggaagcgtac aacctgttca aacggctgta
     1381 cttcgagcag ccgatcttca tgtccgagcg ccgcgattat gagacaaccc gtgaccagct
     1441 gttaaaggac aaactcgaga aggcgaaagc ccagtggaac ctggtgctgt ctctcgcgca
     1501 gcgattggcc tcacgtgaag caccgtctcc gaagcagccg ccggccgaag acaatatcca
     1561 agccatcaaa gatcagattc atgcggagat cgacaagctc atcgacatgt acaacgacgt
     1621 gttgaaagat gagccacaac atcttcaggc actgaaaggg ctggtctact gctacaccga
     1681 ccggagcgag accgcccagg caagtgcaca tcaggaacgt ctgaaactgg cgcacagtta
     1741 ttggaacgat ctgggatcgc gccgtgccgc cgtgtgtctg aacgaagccc ggcgcgtact
     1801 cgatgaagag aagaaagatc tcgccatcga tctcatcaac cgcgggctcg ataccgcacc
     1861 ttcccattcg ggtcttctgg tgcagaaagc cgagattctg cgaggtctgc agaaattcaa
     1921 ggaagcggca caatgtatcg aggtattgct gcgcgaaagt cccaatgatt ctgaaggcct
     1981 caagatgaaa aagaacctgc agaatgaccg actcgaagag tcgatcaaac gcgggtggct
     2041 gatgatcaag gaaatggagt ccggaactca gacggaaagc cagacacgca ccaccctcaa
     2101 agaggcgatg gacctgttct ttacctgcct tgatatcgat gccaacagct tgcgcgcact
     2161 cgagggcatc tatcgttgtc agctgttttc gaacaatccg ctgaaagcaa aaaagacgct
     2221 tgagcgcatc aaggaaatcg attcgaccta ccgcgtcgga accgtcggct caacccagcc
     2281 gccggcggca gatcaggctg accaatgctt cgtcgccacc cgcatctttg gcccccggca
     2341 tccgacgacc tgccgattgc gtgaatttcg tgacacgcat ttacgtcata caatcagcgg
     2401 tcgcgtgttc atacgatggt acaaacgtat cggccctcgc ctggccgcct tgccgcgccg
     2461 aagtccgacc gtgcgactgc tggcgcgcgt gttgaaaacc attcttccac actgaagacc
     2521 ggtttgccgg gcggtattgt cgccacaaca actgggactc acgccctgac aaccggtgcg
     2581 tgaactgagc cgttgccggt tgtttcagtt cagaccgctg gcgagcgcga gcttgattga
     2641 gttgaggcgc gtctgcttcg aatcggcacg cgaaagcaga ataatcggga ccttcgcgcc
     2701 gacgatcagg ccgccggcag gaacgttggc aaaatacatc agagacttgc ccaacacgtt
     2761 tccggtatcg atatcggggc agacgagaat gtcggcctgt ccgaccacat cgccgtgcaa
     2821 ccctttgtgg tgcgccgatt cgacggaaat ggcgttgtcg agcgcgagcg gcccttcgac
     2881 ctgacacgag ccaaactgtt tgcgctcgcc cattttggcc agaaccgcgg catcaaccgt
     2941 gctgggcatc ttgagattca ccgtctcgag cgctgacagc accgcgacct tgggcttggc
     3001 gtctcccaga cggcgtgcca gatcgaccgc gtttgccaga atcgcctgtt tctcggccag
     3061 ggtcgggtgg gggaccatgc ctccatccgt cagatacttg aaacgccctt cccattcgta
     3121 gaccagtacg tgggaaagga catcgccggc tttgatgccg ttttccgagt tcaacaccgc
     3181 cttcagcagc ttgggcgtcg caatggtgcc cttcatcagg gccttgccgc gccccgaacg
     3241 aatgagtgcc acggacttgg ccgccgcatc ctcttcgcct tcggcatcga cgatctcggg
     3301 ttcgaagttc ttggccacct gcttcaccag ctcagtgata cgagcccggt taccgacgaa
     3361 gacgcaatca acgtatccca tctccatggc ttcctgcgcc gccaggattg cctcctggtc
     3421 ttcgggcatg cagataatca ggggaacgcg attcgcctgc agtttctttt ccagttccga
     3481 aaagttcatc tctcaatctc cttatcgata gtccttgacc atggatgggt tctgtaatgc
     3541 cgtgaccgcg tgctctgcca gtgccttgag ttcgttctgg cccggcttga tgaccacggg
     3601 cgcaataaat tggacccgct cggtgatcca gtcggtgacc gtcttggaat aggcaacgcc
     3661 gccggtcaga atgatggtgt cgacttttcc tttgagaacg gtggccatgg cgccgatctc
     3721 cttggcgatc gtgtaagcca tcgcctcgag taccagtcgc gctttctgat ctccgctgtc
     3781 aatgcgtttc tgcacttctc gaacatctgc ggtgccgaga tgcgcgatca agccgccctt
     3841 gccgatcagc atcttgcgta gttccggcag cgtatgcttg ccggaacagg ccagtttcag
     3901 cagcccggtc agcggcagag tgcctgcccg ctcgggtgaa aatggtccgg aatcattggc
     3961 attattgacg tcgatctgtc ggccctggcg atgggcgacg atcgagatgc cgccgccgag
     4021 atgcgcgacg atgagattga ggtcctccgg gttgcgcttc tggtcgcgcg ccacttcgcg
     4081 aatgaccgcc ttgcagttga gcgcatgggc cagcgagatg cgcggcagat ccgggtgtcc
     4141 gctgatgcga gccaggtcgt cgaactcgtc gaccgagacc gggtcggcga tgaaagccgg
     4201 aatacccgct tcacccgcga tgcgatacgc caggagccca cccagaatcg acgcatgatc
     4261 cacgagtttg cctgagcgca gatcgttcag cagagcgtca cccaaacgat acacaccgcc
     4321 ctccatgggc ttgagagggc cgccacggcc gatgaccgcc tgcagatccc gcggttgaat
     4381 cttccaggtt tcgagggccc ggcgtacttc cccaaggcga aagtcgagct gctcgagatt
     4441 ggaactgaac ttcgacagct ccgaacccgg gtgtgtgatt tcctgctctg ctcgtgattg
     4501 agtgccatca aacagggcga ccttcgtcga ggttgacccc gggttgatga ccagcagcaa
     4561 tggttgcttc atgatccctt cctttcgatg tctctttctt tattctctca ccgacgatgt
     4621 tgccctcggc agcaggcgcg accagggcat ctgttcccag gccagctgat ctgccggaca
     4681 cagggggggg agcgtctcct ctggtctgaa ccaggccgcc tgctctgccg gccactttcg
     4741 agtcaggttt tcgttccccg gtaccggtgt gcaacggaca aaatgcaggc gtacacgctt
     4801 gtttgggta
//
LOCUS       DAORHD010000185         4806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18586_length_4806_cov_3.324142, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000185 DAORHD010000000
VERSION     DAORHD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18586_length_4806_cov_3.324142"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PKO06_05295"
     CDS             complement(<1..1393)
                     /locus_tag="PKO06_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNV69092.1"
                     /translation="MTHQPPASIVFTNKARCRDCHRCLRVCPVKAIRMQNGQAHVVPE
                     RCISCGTCIRECPQGAKTYRRDLERAKRVMHEKRLTAVSVAPSFAGILQTWERRRLAS
                     ALRRLGFRHVAETSIGAALIARETARLAESRDSPIIGTACPVVVNWFEARAPEALSAL
                     APVVSPMVAHARYLRQKLGPDIGLVFVGPCVGKKAEADRPEHQGLIDAVLTFEELDEW
                     LKQEGVTLNGCEESDFDEVAPVDAVLFPLAGGQARAGRLDTDLLSERTITTTGVDELH
                     DILPLLHTQSASGRSRLIEPLFCRQGCINGPACGRPGSVLERRRGLIEYVAELRERPS
                     WIAPETQPNLCTSYAANVSARLRDASEEEVRAVLEKTGKGKPEDQLNCGACGYGSCRD
                     KAIAVIQGMAEPEMCIPHMRRLAEQRTDRIIETSPNGIVICGERLEILHMNQAFRRMF
                     LCSEAVLGKHISYL"
     gene            complement(1434..3482)
                     /locus_tag="PKO06_05300"
     CDS             complement(1434..3482)
                     /locus_tag="PKO06_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNV69093.1"
                     /translation="MNTQTPTNTLVIDGRNVPINGERNLLEVIRKADIELPTFCYHSE
                     LSVYGACRMCLVDVDGRGIMAACSTPPESGMKVRTVTEEIREIRKISLELLLANHDQK
                     CPTCSKSAACKLQDLARKVGVTDVRFKPVQRRQPVDRSSPSLVRDPNKCILCGDCVRM
                     CREIQGIGAIDFAHRGKSVAVLPSFGKDLAAVECVNCGQCAAVCPTGALTPRDELDGV
                     WKALSNPRKKVIAQIAPAVRVGLSESLGLAPEMVTTGTIVAALKRIGFAKVFDTAFAA
                     DLTVIEEAEEFLKRKKAGKQLPQFTSCCPAWVKFAEQYYPTLLPNLSTCRSPQQMFAS
                     VARRVLPAQMDVKNEDLVVVSIMPCTAKKYEADRPEFATAGHPEVDYVITTQELVRMI
                     ETSGLDLQALQPESLDLPFGYKTGAGVIFGVSGGVTEAVLRYAAEKIGGAKLDNVDFH
                     EVRGEKGRREATVRVNGTEVRLCVVHGLAQARALAQEVVDGKAAYDLIEVMACPMGCI
                     GGAGQPVARDREALKARGKGLYEADKMLMLHKAQENGFVSQLYEKTLHEVGGHEAHQL
                     LHTQYQSRRRLDEKGMSLLPSTGADRLPVSVCVGTSCYVRGAQTLLHKLIRHVVDEGL
                     ADRVEVNASFCFEACDRGPTVRIGERLIHKCTFELACAILKEEVAAWERVHAQPQTVG
                     "
     gene            complement(3499..>4806)
                     /locus_tag="PKO06_05305"
     CDS             complement(3499..>4806)
                     /locus_tag="PKO06_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005038260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNV69094.1"
                     /translation="RGGGGFPTGKKWDLTRVQPGPKRYVICNGDEGDPGAFMDRSVME
                     GNPHSVIEGMMIAARAIGADEGYVYVRAEYPLAVRRVRHAVEEAQRLGLLGKNLFGSG
                     CDFELHVMEGAGAFVCGEETALMASIEGQRGMPRPKPPFPAKSGLWGKPTVINNVETL
                     ASVPLILRDGAARFRSVGTPSSPGTKTFALTGHVANTGLIEVPFGATLREIVYNIGGG
                     VTDAAGQIQGTTFKAVQIGGPSGGCLTEANLDLPLDFDSLRSIGAMVGSGGLVVMNKQ
                     TCMVQVARFFMQFTQNESCGKCVLCREGTRQMLALLDDIIEGRGTRETLDLLETLSGA
                     VIKGSLCGLGKTAPNPVVSTLRQFRAEYEAHVFQKRCPTGNCKALVSLSIDSAKCKGC
                     TLCARKCPVNAISGAVRQPHKIDGSLCIKCGACVTACKFGAIS"
BASE COUNT          931 a         1610 c         1324 g          941 t
ORIGIN      
        1 tcagatacga tatgtgtttt cccagaaccg cctcgctgca caggaacatg cggcgaaacg
       61 cctgattcat atgcagaatc tcgagccgct caccgcagat gacgatccca ttcgggctgg
      121 tttcaatgat ccgatccgtg cgctgctctg ccagacgcct catgtgcgga atgcacattt
      181 ccggttcggc catcccctga atgacggcta tcgccttgtc gcgacacgat ccgtagccgc
      241 aggccccgca gttcagctgg tcttccggtt tcccttttcc cgtcttttcg agaaccgctc
      301 gcacttcctc ttctgaagca tcacgcaacc gcgcggagac attcgccgcg taactcgtac
      361 acaagttcgg ttgcgtctcc ggagcaatcc aggaaggtcg ctcgcgcagt tcggcaacat
      421 actcgatcag ccctcgacgc cgctccagaa cacttcccgg ccgaccgcag gcaggcccat
      481 tgatacagcc ctggcgacag aacagcggct caatcagtcg cgaacgcccg ctcgccgact
      541 gggtatgcaa aaggggcaga atatcgtgca actcgtccac accggtcgtg gtgatcgttc
      601 gctccgacag caggtccgtg tcgagtcgcc ccgcccgcgc ctggccaccc gccagcggaa
      661 agagcacggc atcgacgggg gcaacttcgt caaaatcgct ttcttcacaa ccattcagag
      721 taacgccttc ctgtttcagc cattcatcga gttcttcgaa cgtcagcaca gcgtcgatca
      781 acccctgatg ctcggggcgg tcggcttcgg ctttcttgcc gacacagggg ccgacaaaga
      841 ccagtccgat gtccgggccg agcttctggc gcagataacg cgcgtgcgcc accatgggcg
      901 agacaaccgg cgccagggcc gacagcgctt cgggagcccg cgcctcgaac cagttcacca
      961 cgaccgggca ggcggttccg atgatcggac tgtcgcgact ctcggcgaga cgagccgtct
     1021 cacgagcgat cagagccgct ccgatcgacg tttcggccac gtggcgaaaa cccagccttc
     1081 gcagagccga ggccaggcgg cgccgttccc atgtctgaag aataccggca aaggaaggcg
     1141 ccacgctgac cgcggtcagt cgcttctcgt gcatgactcg tttcgcccgt tccagatcgc
     1201 gacgatacgt cttggccccc tgcggacact cgcgaataca ggtgccgcac gagatgcaac
     1261 gttccggcac cacatgcgcc tgcccattct gcatgcgaat cgccttgacc ggacagaccc
     1321 gcagacagcg gtgacagtcg cggcagcgcg ctttgttggt gaacacgatc gaggccgggg
     1381 gttgatgagt catgtgtttg atcccgacgg gaaagttcct cgtggtgccg gtatcagccg
     1441 accgtctgag gttgagcgtg aacgcgttcc catgcggcca cctcttcttt cagtatcgca
     1501 caggcaagct cgaacgtgca cttgtgaatg agtcgctccc cgattcgcac cgtcggaccg
     1561 cgatcacacg cttcgaaaca gaacgacgca ttcacctcga cacgatcggc cagcccctcg
     1621 tcgacgacgt gcctgatcag cttatgcagc aaagtctgcg cgccccgcac atagcagctc
     1681 gtaccgacgc agacactcac cggcagccga tcggctcctg tcgaaggcag cagcgacatg
     1741 cccttttcat caagacgacg gcggctctga tactgcgtat gcaacagctg atgggcttcg
     1801 tgaccgccga cctcgtgcag ggttttctcg tagagttggc tgacaaagcc gttttcttga
     1861 gccttgtgca acatcagcat cttgtcagct tcatacaacc ccttcccgcg ggctttcaga
     1921 gcttcgcgat cacgggccac cggctgcccc gccccgccga tgcaacccat ggggcaggcc
     1981 attacctcga tcaggtcata ggccgctttg ccatccacga cttcctgagc cagcgcccgc
     2041 gcctgggcca atccatgcac cacacacaga cgaacttcgg tgccgttcac tcgcaccgtc
     2101 gcctcgcgcc gccccttctc gccgcgcacc tcgtgaaagt cgacattgtc gagtttcgcg
     2161 cccccgatct tctcggcggc atatcgcagc accgcctcgg tcacgccgcc gctgacaccg
     2221 aagatcacgc ccgctccagt cttgtagcca aacggcagat cgagcgattc cggctgcagc
     2281 gcctgcaaat cgagccccga tgtttcgatc attcgtacca gttcctgtgt ggtaatcaca
     2341 tagtcgacct ctgggtgtcc ggccgtcgca aattccggac gatcggcctc atactttttc
     2401 gcggtgcagg gcatgatcga cacgacgacc agatcttcat tcttcacatc catctgcgcg
     2461 ggcaacaccc gccgcgccac cgaggcgaac atctgctgcg gagatcggca ggtagagagg
     2521 ttcggcaaca aggtgggata gtactgttcc gcgaacttca cccaggccgg acagcaggaa
     2581 gtaaattgcg gcagttgctt tcccgctttc ttgcgcttga gaaactcttc agcctcttcg
     2641 atgaccgtca ggtcggcagc gaatgccgtg tcgaagactt tcgcaaaccc gatgcgcttc
     2701 agcgcggcga cgatggttcc ggtcgtgacc atttcgggcg cgagtccgag cgattcactc
     2761 aagccgaccc tcaccgccgg agcaatctgg gcgatgactt tcttacgcgg gttgctcaga
     2821 gctttccaga ccccgtcgag ttcgtcgcga ggtgtcaggg ctcctgtcgg gcagaccgcg
     2881 gcgcactggc cacagttcac gcattcgacc gcagccagat ctttgccgaa actgggcaac
     2941 actgcgaccg actttccccg atgcgcgaaa tcgatcgcgc cgatgccctg aatttcgcgg
     3001 cacatccgca cgcagtctcc acagagaatg cacttgttcg gatcacgcac gagcgagggg
     3061 ctcgagcgat cgaccggctg ccggcgctgc accggcttga accgcacatc ggtcacgccc
     3121 accttacgcg ccagatcctg cagtttgcag gccgcgctct ttgagcaggt cggacacttc
     3181 tggtcgtggt tcgccagcag cagctcgaga gagatcttcc gaatttcccg aatctcttcg
     3241 gtcaccgtgc gcactttcat gcccgactcc ggtggcgtcg aacaggccgc catgatgccg
     3301 cgtccgtcga cgtcgaccag gcacatgcga caggctccgt agacggaaag ttccgaatga
     3361 tagcagaatg tcggcaattc tatatcggcc ttgcgaatga cttccagcag gtttcgctca
     3421 ccgttgatcg gtacatttcg accgtcgatc accagtgtat tcgttggggt ttgagtattc
     3481 ataatctttc tccgtggttc aggaaatcgc cccgaatttg caggcggtga cacaggctcc
     3541 gcacttgatg cagagcgagc cgtcgatctt gtgtggttgg cgaacggcac ccgatatggc
     3601 attgaccgga catttgcgcg cgcagagcgt acaacccttg cacttcgcgg agtcgatgct
     3661 gagcgacacc agggctttgc agttaccagt cgggcagcgc ttctgaaaga catgcgcctc
     3721 atactcagcc cggaactgcc gtaacgtcga gaccacgggg ttcggtgccg ttttccccaa
     3781 accgcagagc gaccccttga tgaccgcccc ggaaagcgtc tcaagcaggt caagcgtctc
     3841 tctggtgccg cgaccctcaa tgatgtcatc gagcaaagcc agcatctgac gggttccttc
     3901 gcggcagagc acgcacttgc cgcacgactc gttctgggtg aactgcatga aaaaccgcgc
     3961 cacctgaacc atgcaggtct gcttgttcat gaccaccaac ccgcccgagc cgaccatggc
     4021 gccgatactg cgcagcgagt cgaaatcgag cggcagatca agattcgcct cggtgagaca
     4081 cccacccgac gggccgccga tctgcaccgc cttgaacgtc gtgccttgaa tctgtcctgc
     4141 cgcatcggtg acacccccac cgatgttgta gacgatctcg cgcagcgtcg ctccgaacgg
     4201 cacttcgatc aacccggtat tggcgacgtg gccggtcagg gcaaacgtct tggtacccgg
     4261 tgatgagggc gtaccgacac tgcgaaagcg ggcggcacca tcacgcagga tcaatggcac
     4321 gctggccagg gtctcgacgt tgttgatgac cgtcggtttg ccccagagac cgctcttggc
     4381 cggaaacggc ggtttcgggc ggggcatgcc gcgctgccct tcgatcgagg ccatcagggc
     4441 cgtctcttca ccacagacga acgctcccgc gccttccatg acatgcagtt cgaagtcgca
     4501 gcccgagccg aacagattct tcccgagaag tccgagtcgc tgagcttctt cgaccgcatg
     4561 ccgaacccgt cgcacagcca acggatattc agcccgcaca tacacgtacc cttcatccgc
     4621 cccgattgcc cgcgccgcga tcatcatgcc ttcgatcacc gaatgcgggt tcccttccat
     4681 cacgctgcga tccatgaatg cgcccgggtc cccctcgtcg ccgttacaga tcacgtatct
     4741 cttcgggccc ggctgcacac gggtcaggtc ccacttcttc ccggtcggga acccgccgcc
     4801 gccacg
//
LOCUS       DAORHD010000186         4799 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18618_length_4799_cov_2.885540, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000186 DAORHD010000000
VERSION     DAORHD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18618_length_4799_cov_2.885540"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2799
                     /locus_tag="PKO06_05310"
     CDS             <1..2799
                     /locus_tag="PKO06_05310"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69095.1"
                     /translation="LQQFNETLEKERGFRLGFRTGLHCGQVVVGMIGGRLDILGDAVN
                     VTARIEEQASEGEVLISRDLATLLRDHFLLGPVTQVKVKGKDEPIKVQVLKGEAPFHQ
                     RQILNHKTPFIGREPEVQFMTGFFQKALATGMPAVLLYQSPPGCGKSRLLAEFEASLA
                     GQGLCRLFLNCVFSPNLTMNIQILRSFLQINRSITTREDLAAHLRRFLVHEEPETLAL
                     AALYIAALLGFSLSEEESIHAIAGTPEETWNRALALFAGIFSRLTRHSPVVILLDDIQ
                     WIDEGSMRVLTTLTAQVTGPFCVIAMGRPEKRISDALVGCPGVLVRDLLPLSPEKSLE
                     LVNGLLSTLQSVPQDLIDQVTTIAQGNPFFIEEIIINLRDKEVIRNDDDQWSVDSQRL
                     GQLHIPTQIEMIVQARLDLLPKSDLELLKKASVMGRNFFWEPVARREGGRLPAEGIAN
                     FLNRGIILLGPDDQDLGIRQYRFSHEIIREVLYARLTRRQKHSNHLLMAEWFRSRPLE
                     EAVQLRYLAYHYEHAEHDTEAIECAMQGGSLALRRFEILEALRFFGQVDRLLRRNVAR
                     FDQPTLARFLEQYAEALLLDGKPQTVIEVIDRHRSKLDERSIHAFRICLKKLEALEKI
                     GDESTWQALLASSAVALKACSSRRSKDWKRLQAGFAVRQGEYCRRHERYAEAEEQFAK
                     ALRIAEELHDKWEIAACHRSLGVISFHLGARAKAFEHHRLTLIALEELNNWEAVQTYY
                     ENASQAYCVKFDLAQSRQEFEAQLAQARAEADQKIMAVCLNYLGVISRYQRDFATAIL
                     YSREACSLREKSGDWYGQMLCLGNMALAQRSLGRLDLAGDLFEQALSLCRAHDDPQST
                     SFFLISLATHWSIQKRFDKAIGFLRQAYDLRRRNGDDGMLAAIEKEIWNCIQKRDALQ
                     ESNTPHAHGDGCKPCVRG"
     gene            complement(3446..3790)
                     /gene="mazF"
                     /locus_tag="PKO06_05315"
     CDS             complement(3446..3790)
                     /gene="mazF"
                     /locus_tag="PKO06_05315"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HNV69096.1"
                     /translation="MKYIPSRGDLVWISLNPQSGHEQAGRRPCLVLSPKSYNSKVGMA
                     VFVPITNQIKGYPFEVIIPTGTKIRGAVLADQIKNLDWQTREAELICRIPQDTFDEVI
                     QKLSALLFQQTE"
     gene            complement(3791..4033)
                     /locus_tag="PKO06_05320"
     CDS             complement(3791..4033)
                     /locus_tag="PKO06_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV69097.1"
                     /translation="MKIKVQMWGNSLALRIPKPFACEFNLKHNSEVDVTIEDGHIIVK
                     PVTVNPFVLDEMLKKVNKSNIHENIDFGLPQGRENW"
     gene            complement(4228..4467)
                     /locus_tag="PKO06_05325"
     CDS             complement(4228..4467)
                     /locus_tag="PKO06_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNV69098.1"
                     /translation="MSNISLRLPNSLHEFVRRLAEKDGVSINQFISIAVAEKVSALST
                     EDYLEKRAEKGNRQDFLKIMKKVPIKKPEPEDEIF"
     gene            complement(4464..>4799)
                     /locus_tag="PKO06_05330"
     CDS             complement(4464..>4799)
                     /locus_tag="PKO06_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV69099.1"
                     /translation="DEGRYENNVSTPLVLEYEATLKRQKEVLSLSYSEIEKFIDYLCS
                     ISNRRRVYFLWRPTMNDPNDEMVLEVAVSSRVEFIVTFNKRDFSNAAKFGIIVLNPNE
                     FLKILEVKK"
BASE COUNT         1102 a         1272 c         1268 g         1157 t
ORIGIN      
        1 cttcagcagt tcaatgaaac gctggaaaag gaacgcgggt tccgtctcgg tttccgtacc
       61 ggtctgcatt gtggtcaggt cgtggtcgga atgatcggcg gacggctcga tattctcggc
      121 gatgccgtga acgtgacggc tcgtatcgag gaacaggcat ctgagggtga ggtgctcatc
      181 tcgcgtgacc tcgcgacgct tctgcgagat catttcctgc tgggtccggt gacgcaggtg
      241 aaggtgaagg gcaaggacga accgatcaag gttcaggtgc tgaaaggcga agccccgttt
      301 catcaacgtc agattctgaa ccataaaaca ccgttcatcg gtcgtgaacc cgaagtgcag
      361 ttcatgaccg ggttctttca gaaagcgctg gccaccggta tgccggcggt tctgctctat
      421 cagagcccgc ccgggtgcgg caaaagtcga ctgctggccg aattcgaggc gagtctggca
      481 gggcaggggt tgtgtcggct gtttctgaac tgtgtgttca gcccgaacct gacgatgaac
      541 atccagattc tccggtcgtt tctgcagatc aaccgcagca tcacgacccg tgaagacctg
      601 gcggcccatc tgcgtcgatt cctggtacat gaggagcccg agacgctcgc cctggccgca
      661 ctgtatatcg ccgctctgct cgggttttcc ctgagcgaag aggaatcgat tcacgcgatc
      721 gccggaaccc cggaagaaac ctggaaccgt gctctcgcgc tgttcgccgg aatcttctcg
      781 cgcctgacgc gccactcgcc tgtcgtgatt ctgctcgacg atattcaatg gatcgacgag
      841 ggatcgatgc gggtgctgac gaccctgacg gcgcaggtga ccggaccatt ctgcgtcatc
      901 gcgatggggc ggcccgagaa acgcatcagc gacgctctcg tcggatgtcc gggagtgctt
      961 gtgcgcgacc tgcttccgct ttcaccagaa aaatctctcg aactggtgaa cggcctgctg
     1021 tcgacgctgc agagcgtgcc gcaggatctg atcgatcagg tgaccactat cgcgcagggc
     1081 aacccgttct tcatcgagga gatcatcatc aatctgcgcg acaaagaagt cattcgaaac
     1141 gacgatgatc agtggtcggt cgactcgcag cgactcggac agttgcacat tccgacccag
     1201 atcgaaatga tcgtgcaggc tcgccttgat ctcctgccga agtccgatct cgaactgctg
     1261 aaaaaggctt ccgtcatggg ccgcaacttc ttctgggaac cggtcgcgag gcgtgagggc
     1321 ggcaggcttc cggccgaggg catcgcgaac tttctcaatc gcggcatcat tctgctgggc
     1381 cctgatgatc aggatctcgg catacgtcag tatcggtttt cgcacgagat cattcgcgag
     1441 gtgctgtatg cgcgcctcac ccgacgtcag aagcacagca atcacctgct catggccgaa
     1501 tggtttcgct cacgtccgct cgaagaggcg gtgcagttac ggtatctcgc ctaccattac
     1561 gaacacgccg agcacgacac tgaggcgatc gagtgcgcga tgcagggggg aagcctggcg
     1621 ctgcgccgct ttgagattct cgaagcgctg cgttttttcg gtcaggtcga tcggctgctg
     1681 cgtcggaacg tggcgcgctt cgatcagcca actctggcgc ggtttctcga gcagtatgcc
     1741 gaagcactgc tgctcgacgg caagccgcag accgtcatcg aggtgatcga ccgtcaccgc
     1801 tcgaagctcg acgagcggtc gattcatgcc tttcgcatct gtctcaagaa gctcgaagcc
     1861 ctggaaaaaa tcggcgacga gtccacctgg caggcgctgc tggcgtcgag cgccgtcgcg
     1921 ctgaaggctt gttcgtcgcg ccgcagtaaa gactggaagc gtctgcaggc gggattcgcc
     1981 gtcaggcagg gcgaatactg tcgaaggcat gagcggtatg ccgaagccga ggaacagttt
     2041 gccaaagcgt tgcggatcgc ggaagaactg catgacaagt gggagatcgc cgcctgccat
     2101 cgcagtctcg gcgtgatcag ctttcatctc ggagcccggg ccaaagcgtt cgaacatcac
     2161 cgcctgacct tgatcgcgct tgaagaactg aacaactggg aagccgttca gacctattac
     2221 gaaaacgcat cacaggcgta ttgcgtgaag ttcgatctgg cacaatcgcg ccaggagttc
     2281 gaggcgcagc tggcgcaggc acgcgcagag gctgaccaga agatcatggc ggtctgtctc
     2341 aactatctcg gggtgatttc ccggtatcag cgtgatttcg cgaccgcgat tctttactcg
     2401 cgagaagcct gttcactgcg cgaaaagagc ggcgactggt atggacagat gctgtgtctg
     2461 ggcaacatgg ccctggcgca gcgcagcctc ggacgtctcg atctggccgg cgatctcttc
     2521 gaacaggcac tcagtctgtg ccgcgcacac gatgacccgc agagcaccag cttctttctc
     2581 atttcgctcg ccacgcactg gtcgatacag aaacgcttcg acaaggcgat cgggtttctg
     2641 cgacaggcct acgatctgcg ccgccgcaac ggagacgacg gcatgctggc tgcgatcgaa
     2701 aaagaaatct ggaattgcat tcagaagcga gatgcgctgc aggaaagcaa cacgcctcat
     2761 gcacacggtg acggatgcaa gccctgcgtc cgtggttgat cccgcgctcc agacggatcg
     2821 cagaatgcgg ccattcactc accggtagca aggggattct ccacgatgca gagggcgttc
     2881 atcccagtgt tcctgaccat cctgcttctc gccacttcgg gaagggatct gattgccggc
     2941 gagactgacg tcacgaaaat tctccgtgat tgtaaacatg aggttcatat ggtgagtacc
     3001 actttcctgg atgtaagtgc attgaatatc caccgggtct taacactctg tgaatttcaa
     3061 gaaaacattc cttcttattt tgagcgtgct ccaacacact tgtgctcacc acaaaatcga
     3121 atgtgttttc gtcaaatggt aactggtacg gatcttgagt aatccttcct aatctttcta
     3181 ccttaaaatg atcatctggc caataacttt caacatcaca accgtaggca tcgaatccgc
     3241 gctgaaggaa tagatcaacg agatttcctc gtccacatcc aaaatcaagt attctcatag
     3301 gcttagaacg gtcgatgccg cataactgtt ctattgccgc tgtaagaaaa gtgtcggtgg
     3361 atctatcttg cattacagac ccttaagaca gtcgtaccaa tgtgcccaac gccctgccct
     3421 ccgctcgatc gctctgttat atcaattatt ctgtttgttg aaatagaagg gcagacaact
     3481 tttgtatcac ttcatcgaaa gtatcttgtg gaatcctgca gatcaactcc gcctctcttg
     3541 tttgccagtc gaggttttta atctggtcag caagaactgc tccgcgtatc tttgtacctg
     3601 ttggaatgat aacttcgaac ggatagcctt taatctgatt tgtgataggc acaaacaccg
     3661 ccatgccaac cttggagtta tatgattttg gtgataacac aaggcaaggg cgtctaccag
     3721 cttgctcgtg accagattgc gggttcagcg aaatccaaac gaggtcaccg cgtgatggga
     3781 tgtacttcat ttaccagttc tctcgcccct ggggtaaccc aaaatctatg ttttcatgaa
     3841 tgtttgattt gttgaccttt ttcaacatct catccaagac aaaggggtta actgtcacag
     3901 gtttgacaat aatatggccg tcttcaatgg taacatcgac ttcggaattg tgtttcaaat
     3961 tgaattcgca ggcaaacggc ttgggaatac gcaatgccaa gctattaccc cacatctgga
     4021 ctttaatttt catatcaagc ctccgtatct actacaaaga tacgaccaaa acaatcattg
     4081 cgcaagggat tttcggtttt tggggtctat cgcccggcct tcagctgacg ccgaacgcag
     4141 tgagggaatt tggggtgggc gcatgcgttg cgtcagcaag gcatgtgacc ggcccaaacg
     4201 ttccgctcga tcgctctgtt ataccattca gaagatttca tcttctggtt ctggtttttt
     4261 aatggggacc tttttcatga ttttcaaaaa gtcctgccga tttccttttt ctgccctctt
     4321 ttctaaatag tcttcagttg acagtgcgga tactttttcg gccacagcaa tagaaatgaa
     4381 ttggttaata gatacgccgt ctttctctgc aagtctacga acaaactcgt gtagtgagtt
     4441 aggaagccga agacttatat tactcatttt ttcacctcca aaatcttgag gaactcattt
     4501 gggtttaaaa cgatgatgcc aaactttgcc gcgttggaaa aatcacgttt gttaaatgta
     4561 acaataaatt caactcttga tgagacagcc acttcgagca ccatttcatc attaggatca
     4621 ttcattgtgg gtcgccaaag aaagtagact cttcttcggt ttgaaataga gcaaaggtaa
     4681 tcaatgaatt tttcaatctc ggagtaagat aaggaaagga cttctttttg tcgtttcagt
     4741 gtcgcctcat actcaagaac aagaggagtc gacacattgt tctcgtatcg accctcatc
//
LOCUS       DAORHD010000187         4787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18687_length_4787_cov_3.454353, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000187 DAORHD010000000
VERSION     DAORHD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18687_length_4787_cov_3.454353"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1008
                     /locus_tag="PKO06_05335"
     CDS             1..1008
                     /locus_tag="PKO06_05335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005993440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69100.1"
                     /translation="MPKTPWTSSVPASTLREAIDSLRQQTEPDFELVIVDDGSTDDGV
                     ALVESIAACDRRVRLIRQGPLGIVAALQRGIQESRGRFLARMDADDVCLPERLGCQRA
                     FLEQHFDIGLVSCLVDHLAVSAEQDGYRRHVDWLNRVITSDEICRARFIESPLAHPSV
                     LFRRELVDRYGGYRAGDFPEDYELWLRWLEAGVRMEKIPRVLLRWRDLPQRLSRTDAR
                     YDLDSFFRVKAGYLARWLAVTNPHHPRVWIWGASRLARRRAELLFAEGISPEGFIEVK
                     PGRVGTSVLGVPVVSRECLPPVGSVFVVVFVSARNAREEIRAVAQRQGYREGEHFVFA
                     A"
     gene            1061..2719
                     /locus_tag="PKO06_05340"
     CDS             1061..2719
                     /locus_tag="PKO06_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNV69101.1"
                     /translation="MTTAALIPMVRHRPAGWSCRSLRDAVLYALAFFLISQLGGAMEQ
                     AQWMNSNDRERAQVMRQRAVDWRNGAIVYQILVDRFAPSTKLEKKRGLYKSPRRLRNW
                     QDLPTRGTYNRDAAVWSQEIDFWGGDLDSLMSKLDYLQELGVEVVYLNPIFLSLTNHK
                     YDAWDYHQVDPMYGTRKDVGRLAKALHERGMKLMLDGVFNHMGRQSPMFQEALRDEKS
                     RYREFFLFTPRSRYGYIGWNDVENLPELNLDSQTVRDYLYAKPDSVVQSYLRQEKIDG
                     WRLDVAFDLGFTYLSELTEAAHAAKPGCSIIGEIWNYPEEWRPAVDAVMNMHGRRLIL
                     GLVQGKLSGPLATEQWQTLCEDAGIDHLLQAWLVLDNHDVPRLAHVLPQFWQQKMARV
                     LQFTLPGSVNLYYGSEVGMEGGDDPEQRAPMRWDLITPDNQTYQLHKALLRIRKQEPA
                     LRYGDYRRLASEKLFTFSRRTASIREAIFVIANPTARAVTEILSLREGKIMSNTPLRD
                     LLSDRVFNSFTGTVEVTVPAHDVLILKPDCTPFPQGYSRYDRVY"
     gene            complement(2790..3521)
                     /locus_tag="PKO06_05345"
     CDS             complement(2790..3521)
                     /locus_tag="PKO06_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69102.1"
                     /translation="MPLRTFPVWTTLLGVCVVTVCVIFGVNLRRASESGPAYLRRLLL
                     AGMTCLWGLGVISPVSAEEAGRWQALGKTWQEADEIATGKRGPFPFNREGKKKILQEL
                     ADLNGTIDALVTDKVVTAVEGELLKLDLKVLVNGVEEKRPKELEVATCYEPMMWTPEL
                     DSLDRLEKRLPLLSRLEPGKVSPEVWNRVIAGLQKDLQILMDPDHLARLKDDKKTQAA
                     EVADKVQKEIDRLGQQSVPVVPEKK"
     gene            complement(3552..4655)
                     /locus_tag="PKO06_05350"
     CDS             complement(3552..4655)
                     /locus_tag="PKO06_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNV69103.1"
                     /translation="MQRSHVLRLISILVFVSQSFFGPGLWAESTASAPPQMTLASATG
                     GLYTGTIGERLSVIVSLQPREADLYGSYRYTTSLRPISLEGKRSTDATWQLKEWMESD
                     VRSETPPDEPPPTGFWEAREDKNGSTVTGFWFSSDRKKKLPLTLRRVAQLQVMNAPHN
                     IEAFFPVFVESGAQTLNEHLASEVRALITEQHAELQAVAAEFVGEGYEDAQERIKHLG
                     ARLSYTVDAWSPAVLSVLVTQWDYQGGAHGNTRFSTINLRRAADGTLSSLRVADLFQP
                     QSPWAERVAELITRDLQRQKASAMEDNARDFATDLTSNTLTWSLTPSALLIHFAPYEV
                     GCYAEGDFHVAIPWKDLRRVLKKDIPGSFFSRP"
BASE COUNT          928 a         1336 c         1490 g         1033 t
ORIGIN      
        1 ctgccgaaaa cgccctggac ttcatccgtg cctgcatcaa cgctgcgaga agcgattgac
       61 agcctgcggc agcagacaga gcccgacttc gaactggtga tcgtcgatga cggttcgaca
      121 gatgacggcg tggctctggt cgaaagcatc gccgcctgcg atcgtcgggt gcgtttgatc
      181 aggcaggggc cactcgggat cgtcgcagcg ctgcaacgcg ggattcagga aagtcgtggg
      241 cggtttctcg cccgcatgga cgctgatgac gtctgccttc ccgaacggtt gggctgtcaa
      301 agagcctttc tcgaacagca tttcgacatc ggtctggttt cttgtctggt cgatcacctt
      361 gcggtgtccg ccgagcagga tggctatcgc cgtcatgtcg actggttgaa ccgtgtgatc
      421 acctcggatg agatctgccg cgcccgattc attgagtcgc cgctggcaca tccgtcggtc
      481 ctgtttcgcc gggaactggt tgaccggtat ggcgggtacc gggccggtga tttcccggaa
      541 gattacgagc tctggttgcg gtggctcgaa gccggggtgc gcatggaaaa gattccacga
      601 gtgctgctgc gctggcgcga cttgccgcag cgcctgtcaa gaaccgacgc gcgctatgat
      661 ctcgattctt tctttcgggt caaagcgggg tatctggcgc gttggctggc agtcaccaat
      721 ccccaccatc cacgggtctg gatctgggga gccagtcgtc tggcccggcg tcgtgccgaa
      781 ctcttgtttg cggaagggat cagcccggag ggattcatcg aagtaaagcc gggtcgcgtg
      841 ggaacaagcg tgcttggggt gccggtggtg tcgcgtgagt gtctgccacc ggtgggctct
      901 gtgtttgtcg tcgtgttcgt cagtgcccgc aatgcccgcg aagaaattcg tgcggtagcg
      961 cagcgacagg ggtatcgtga aggggaacat ttcgtgttcg ctgcctgaag tgaaggggcg
     1021 aacgctccgt tctgatccgg atctccagga ggataagagt atgacgactg ctgccctgat
     1081 acccatggtt cgccatcggc ctgccggttg gtcttgtcga tcgttgcggg atgcggtact
     1141 gtatgcactc gcgtttttcc tgatctcgca gctggggggt gccatggaac aggcgcagtg
     1201 gatgaactcg aatgatcgtg agcgcgcgca ggtcatgcgg cagcgggccg tggactggcg
     1261 caacggcgcg attgtctacc agatccttgt ggatcgtttc gcgccttcaa caaagctgga
     1321 gaagaaacgc ggcttgtaca aatctccgcg acgattgcgc aactggcagg acttgccgac
     1381 caggggaacg tacaatcggg atgcggcggt ctggagccag gagatcgact tctggggtgg
     1441 agatctcgac agtctgatgt cgaagctgga ctatctgcag gagttgggcg tcgaagtggt
     1501 gtatctcaac ccgatcttcc tgtcgttgac caatcacaaa tacgacgcct gggactacca
     1561 tcaggtcgat ccgatgtatg gcacccgcaa ggatgtcggg cgcctggcga aggcgctgca
     1621 tgagcgtggc atgaagctga tgctcgatgg ggtcttcaat cacatggggc gtcagagccc
     1681 gatgtttcag gaagccctgc gcgacgagaa gagtcgctat cgcgagttct ttctgtttac
     1741 tccacgcagc cgttatggct acatcggctg gaacgacgtc gaaaacctgc ccgagttgaa
     1801 tctcgacagc cagaccgtgc gcgactacct gtacgccaaa cccgactctg tggtgcagtc
     1861 ttacctgcgc caggaaaaga tcgacggctg gcgactcgat gttgccttcg atctcggttt
     1921 cacgtatttg tccgagttga ctgaggcggc gcacgcggcc aaaccaggtt gctcgatcat
     1981 cggtgaaatc tggaactacc cggaagagtg gcgaccggcc gtcgatgccg tgatgaacat
     2041 gcatgggcgt cggttgattc ttggtctggt gcagggcaag ctttcaggac cgctggcgac
     2101 ggagcagtgg cagaccttgt gcgaggatgc cggtatcgac catctgttgc aggcgtggtt
     2161 agtgctcgac aaccacgatg tgccgcgact cgcgcatgtg ctgccgcagt tctggcagca
     2221 gaaaatggcg cgagtcctgc agttcacgct gcccgggagc gtgaatctct actatggcag
     2281 cgaggttggc atggagggcg gcgatgatcc ggaacagcga gcccccatgc gctgggacct
     2341 gatcactcct gacaaccaga cgtaccagct tcacaaagca ctccttcgca tcagaaaaca
     2401 ggagccggca ttacgttatg gcgattatcg tcgcctggcg tcggagaaac tgttcacgtt
     2461 ttcccgccgc acggcttcca ttcgcgaagc catcttcgtg atcgcgaatc cgacggcgcg
     2521 tgccgtaacc gaaattctct cgttgcgcga aggcaagatc atgagtaaca cgccgctgcg
     2581 cgatctgctc tcagatcgcg tcttcaattc gttcaccggt accgttgaag tcacggttcc
     2641 ggctcatgat gttctgattc tcaagcctga ctgtacgccg tttccgcagg gatacagccg
     2701 gtacgatcga gtctactgac cttcgtaata aaagaaatcg cgccgcttcc gattgcacgg
     2761 ataagcggcg cgattcgctt gggggggtgt tactttttct caggaaccac cggaaccgat
     2821 tgttgtccga gtcgatcgat ctctttctgc accttgtcag ccacttccgc ggcctgtgtt
     2881 ttcttgtcat ccttcaggcg ggccaggtgg tcagggtcca tcagaatctg cagatctttc
     2941 tgcagaccgg caatgacgcg attccagacc tctggggaga ccttgcccgg ttcgagccga
     3001 gacagcagag gcaggcgttt ttccagtcga tcgagactgt ccagttcggg agtccacatc
     3061 atcggctcgt aacaggtcgc cacttccagt tccttgggcc gcttctcttc gactccgttg
     3121 accaggactt tgagatcaag tttgagcagt tcgccctcca cggcggtgac gaccttgtcg
     3181 gtgaccaggg catcgatggt gccgttcagg tctgccagtt cctgaagaat ctttttcttt
     3241 ccctcacgat tgaaagggaa ggggccgcgt ttaccggtgg cgatctcatc ggcttcctgc
     3301 caggttttgc ccagcgcttg ccagcgacct gcttcttccg cgctcaccgg agaaatgacg
     3361 ccgagacccc agaggcacgt catgcccgcc agcagcagtc gacgcagata cgccggacct
     3421 gattctgagg cgcgccgaag attgacgccg aaaatgacgc agacggtgac gacacagact
     3481 ccgagcaggg tggtccagac gggaaacgtg cgaagcggca tgagacacct cctgaaaagt
     3541 cggtcagatg attacggtcg agaaaagaac gaacccggaa tgtccttctt cagcacgcgg
     3601 cgcaggtctt tccagggaat ggcgacgtgg aagtctcctt cggcgtagca gcctacttca
     3661 tagggcgcga agtggatcag cagagcgctg ggggtgaggc tccaggtcag tgtgttgctc
     3721 gtcagatcag tggcgaagtc ccgggcatta tcctccatcg ccgaagcctt ctggcgttgc
     3781 agatcacggg tgatcagctc ggccacccgt tcggcccagg gactctgcgg ttggaacaga
     3841 tcagcaacac gcagcgaaga aagggtgccg tccgccgcgc ggcgaagatt gatcgtcgag
     3901 aagcgtgtgt tgccatgggc accgccctgg tagtcccatt gtgtgaccag aacgctgaga
     3961 accgccggtg accatgcatc gacggtgtaa ctcagtcgag cgccgagatg tttgatgcgc
     4021 tcctgggcgt cttcatagcc ctcgcccacg aactccgccg ccactgcctg cagttcagcg
     4081 tgctgttcgg tgatcagagc acggacttcc gaagcgaggt gttcgttcag tgtctgcgct
     4141 cccgattcca cgaaaaccgg gaaaaacgct tcgatgttgt gcggcgcgtt catcacctga
     4201 agctgcgcca cacgccgtaa cgtgagcggc agcttcttct tgcggtcgct gctgaaccag
     4261 aagccggtga cggtgcttcc gttcttgtct tcccgggctt cccagaaacc ggtaggaggc
     4321 ggctcgtcgg gtggggtctc gctgcgaacg tctgactcca tccattcctt cagttgccag
     4381 gttgcgtcag tagaccgttt gccttcaaga gagatcggac gcagcgaggt ggtgtaacga
     4441 taactgccgt acaggtcggc ctcgcgaggc tgtaatgaga caatgacgga gaggcgctct
     4501 ccgatggtgc cggtgtacaa gccgcccgtg gcggaggcga gcgtcatctg cggtggcgcg
     4561 ctggcggtcg attcagccca caggcctggc ccgaaaaacg actgtgacac gaagaccaga
     4621 atgctgatca gacgaagaac gtgagagcgt tgcataggtc acctcaagcg ttgtgatctt
     4681 taccggctgg cgtgattata ccgcagaacg tcaaacgttc gtcaccgcaa ccggattgtc
     4741 cgagtggccc tgtgcgttcg accggtgagg tattgatgaa agcagaa
//
LOCUS       DAORHD010000188         4768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18804_length_4768_cov_4.281774, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000188 DAORHD010000000
VERSION     DAORHD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18804_length_4768_cov_4.281774"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="PKO06_05355"
     CDS             complement(<1..1094)
                     /locus_tag="PKO06_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV69104.1"
                     /translation="MNFRSLIDWIAGRKAPEAATQAATGSERRRYSRLTLTDGEARVV
                     GHEPIPVVNLSFGGVRLALPDSEKSEDWTVGKTLQATIILGSIQFDTGMQVCNVWGRE
                     IGCSFQGSSLGVARLISDYLKPRLLGASLREIKSAQPRAVGKDLRLRWFQGDNGTEVY
                     LWERLDGGLVKEELFFLDYCVSWEESGDGLRTARVAEGSGRQGYGRMDQSSMVFFRIP
                     SHRALRMGQVILLSSSLPPEVRDRFLARMAQEERRLYSRYILGSEDAQIRLTLPTLST
                     HPLSVVNLSLHGIAFLRPEKSSLTSSPAISWEGVLFLNQQEIAVQFKPAFSHGNIMGG
                     SLHFKHLAGSEAVAAYLAPRLLGQSLEEHPA"
     gene            1208..2821
                     /locus_tag="PKO06_05360"
     CDS             1208..2821
                     /locus_tag="PKO06_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015227961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69105.1"
                     /translation="MTIAKELADHIRARYPLLYLVSGEEARAMAMLAETAATVGKSLW
                     VWSASDGLIDPKGQEVKDAKGEPLVSPLKALRWMLRSQERALFVCKDLHLFLEDPFPQ
                     YVTDPIQVRRTLRDISIAFRTSFKTLILLSPLLRIPSELEKTITVFDYPLPTLSEISA
                     LLDEMIAELKKLHGDKVTIDLDADTREQIVKSVSGLTQAEIENVFNRAIVNDLRFDRN
                     DIPFILSEKKQIIRKNGILEYYEVQESFGNVGGLEILKDWLNKRGESFTSRAREFGLP
                     EPKGILLLGVQGCGKSLVSKSISSLWRMPLLRLDMGAIFGKYVGESEENLRRAMRTAE
                     ALAPTILWLDEIEKAFAGVGGSSGDGGVTTRIFGSFITWMQEKTAPVFVIATANSIEN
                     LPPELLRKGRFDEIFFVDLPSQIERESIFDIHLRKRRRDPKAFDLPELGRCSEGFSGA
                     EIEQAVIAGLFEAFHEKRDLQQKDVVESLAATVPLSVTMREQIDKLRSWASQRAKPAS
                     AGSPPVSTTPGSTGRPGLNLFADLDLDRK"
     gene            2831..3355
                     /locus_tag="PKO06_05365"
     CDS             2831..3355
                     /locus_tag="PKO06_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV69106.1"
                     /translation="MAKEKIVYLKDRRKPTAPVSASSDSYAEQVLLLNLKSATVAIAV
                     KRVVEILPHQPLVDIARRDTPVEGVVNVRGRIIPVIDLGLKIVNVPARQSPKPRIIAV
                     EIEKESVGLLIDEVADRLCLLTEPPRPALAGMAQPYFSGVVSVDLRDIPLLDVDQLFT
                     PAERDQIASIRKSF"
     gene            3385..3546
                     /locus_tag="PKO06_05370"
     CDS             3385..3546
                     /locus_tag="PKO06_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNV69107.1"
                     /translation="MAKWKCVVCGYVYDPAQGDPDGGIKPGTPFEQIPDNWVCPICGA
                     GKDQFEKIV"
     gene            3694..4743
                     /locus_tag="PKO06_05375"
     CDS             3694..4743
                     /locus_tag="PKO06_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV69108.1"
                     /translation="MLSRFSVTILVVSALCVVFMPFSSPAQAVTDSTLTAGSPELIHI
                     WRDDCGVAHVVASSTAGAYWGFGYCLARDRLLQLELLRRSVQGTLAEILGKSFLEQDV
                     LARRDGLSQTELQEGLDRADPLFRTALTSFARGINASIDAASRRRQLVHPAFRSLGLS
                     FRPFTELDILEIFAGTMACRYNDFTQELDNLKLLNELVRRLGARSASRIFDDVIPFVD
                     PGVYATLGRREHYMPALPGAVRFGPHPQDLPEGESPTLRQRKRNRELKTIGIPDKSGS
                     YAAVLSLRGQGKPEALLLGGPQMGYFKPSAVYEIGLHTPDFDLVGATPVGYFVILFGA
                     NRHLGFTATAGVAMP"
BASE COUNT          925 a         1407 c         1461 g          975 t
ORIGIN      
        1 gcgggatgtt cctcgagaga ttgtccgagc agacgaggag cgagatacgc ggccacggct
       61 tctgagcctg ccaggtgttt gaagtgcaga gagccgccca tgatgttgcc gtgtgaaaat
      121 gccggtttga actgcacggc gatctcctgc tgattcaaga agagcacgcc ttcccaggaa
      181 atggctggtg atgaagtcag ggagcttttc tccgggcgga gaaacgcgat accgtgcagg
      241 ctcagattga cgacggaaag cgggtgggtg gaaagcgtcg gaagggtcag gcggatctgc
      301 gcatcttcgg aacccaggat atagcggctg tagagacgtc gttcctcttg cgccatacgg
      361 gcgaggaagc ggtcgcggac ttcaggaggc agtgatgacg agagcagaat gacctgaccc
      421 atgcgcaggg cccggtgtga cggtatgcga aaaaacacca tggagctttg atccatgcga
      481 ccgtagccct gacgaccgct cccttccgca acgcgtgcgg tgcgcagacc gtcgcccgac
      541 tcttcccagg agacgcagta gtccagaaag aacaactctt ccttgactaa ccccccatcg
      601 aggcgttccc agaggtagac ttcggtgccg ttgtcgccct gaaaccagcg cagacgaaga
      661 tctttgccga ccgcgcgcgg ttgggcactc ttgatctcgc gcagcgaagc gcccagcagt
      721 cggggcttca gatagtctga gatcaggcgg gcgacaccga gtgacgaccc ctgaaacgag
      781 cagccgatct ctctgcccca gacattgcag acctgcatgc cggtgtcgaa ttgaatgctg
      841 cccagaatga tcgtggcctg aagcgtcttg ccgacagtcc agtcttcgga tttctcgctg
      901 tctggcaacg cgaggcgaac cccgccgaag ctgaggttga ccaccggtat aggctcgtgt
      961 ccgacgaccc gggcctcgcc atccgtcagc gtcaggcggg aataacgccg gcgttcactg
     1021 cctgttgctg cctgcgtggc agcctcgggg gctttgcggc cggcgatcca gtcgatgaga
     1081 gagcggaaat tcatacggaa atagggtcac agaagtcaga tgcctcgagc ataccacacc
     1141 catcgctttc tcatcttccc tttcgtggtt tttctgatac aatgacagcc ttggagaaat
     1201 gccacccatg accattgcca aagaacttgc cgaccacatt cgcgcccggt atccgctgct
     1261 ctatctggtc agcggcgaag aagcccgcgc gatggcgatg ctggcagaaa ccgccgccac
     1321 ggtgggcaag agcctctggg tctggtccgc ttctgacggg ttgatcgacc ccaaaggcca
     1381 ggaagtgaag gacgccaagg gcgagccgct ggtgtcgccg ctgaaagcgc ttcgttggat
     1441 gctgcgctcg caggaacgcg cgctcttcgt ctgcaaagac ctgcatctgt tcctggaaga
     1501 cccatttccc cagtatgtga ccgatccgat tcaggtgcgg cgcaccctgc gtgatatctc
     1561 gattgccttt cgtaccagtt tcaaaacgct gattctgctt tccccgctgc ttcgcattcc
     1621 ctcagaactg gaaaagacga tcacggtgtt cgattatccg ctgccgacgc tgtctgagat
     1681 ctcggcgttg ctcgacgaaa tgatcgccga actgaagaaa ctgcacggcg ataaggtgac
     1741 aatcgacctc gatgctgaca cacgcgagca gatcgtcaag tctgtgtcgg gcctgaccca
     1801 ggctgagatc gaaaacgtct tcaaccgcgc catcgtcaac gatctgcgct tcgatcgcaa
     1861 cgatattccc ttcattctga gcgagaaaaa gcagatcatc cgcaagaacg gcattctcga
     1921 atactacgaa gtgcaggaaa gcttcggcaa cgttggtggc ctcgagattc tgaaagactg
     1981 gctgaacaag cgcggcgagt cgttcacgtc acgggcgcgt gagttcgggt tacccgaacc
     2041 caagggtatt ctgctgctcg gcgtgcaggg atgcggcaag agtctggtca gcaaatcgat
     2101 cagttcgctc tggcgcatgc cgctgctgcg tctcgatatg ggcgcgatct tcggcaagta
     2161 tgtcggcgaa tccgaagaga acctgcgacg ggccatgcgc acggccgagg cgctggcccc
     2221 gacgattctc tggctcgacg agatcgagaa ggcgtttgcc ggggtgggcg gcagcagcgg
     2281 tgacggcggt gtcacgacgc gcatcttcgg cagcttcatc acctggatgc aggaaaagac
     2341 cgcgcccgtc ttcgtcatcg ccacggcgaa cagcatcgag aatctgccgc ccgaactgct
     2401 gcgcaagggg agattcgacg agatcttctt cgtggatctg ccgtcgcaga tcgagcggga
     2461 atccatcttc gacattcacc tgcgcaagcg gcgccgcgat ccgaaggcgt tcgacctgcc
     2521 cgagctgggt cgctgcagcg aagggttttc gggcgccgag atcgaacagg ccgtcatcgc
     2581 cggtctcttc gaggcttttc atgaaaagcg cgatttgcag cagaaagacg tggtagaatc
     2641 cctcgccgcc accgtcccct tatcggtgac gatgcgcgag cagatcgata agttgcgttc
     2701 ctgggcgtcg cagcgggcca aaccggcttc ggcgggcagt ccgcccgtgt caacgacgcc
     2761 gggcagcacg gggcggccgg gtctgaacct gtttgccgat ctcgaccttg accgcaagtg
     2821 aggtgtaacc gtggccaaag aaaagatcgt ctatctgaaa gatcgtcgta agccgacagc
     2881 gccggtgtca gcatcttctg actcgtacgc ggaacaggtg ctgctgctga atctcaagag
     2941 cgcgaccgtg gccattgccg tcaagcgcgt ggtcgagatt ctgccgcatc agccgctggt
     3001 cgacatcgcc cgtcgcgaca ccccggttga gggcgtggtc aacgtgcgcg gccgcatcat
     3061 tccggtcatc gatctcgggc tgaagatcgt gaatgtccca gctcgtcaga gcccgaagcc
     3121 ccgaatcatc gcggtcgaga tcgaaaagga atccgttggt ctgctgatcg atgaggtggc
     3181 cgatcgcctg tgcctgctga ccgaaccacc gcgtccggcg ctcgccggta tggcgcagcc
     3241 ttacttttcg ggcgtcgtct cggtcgatct gcgcgacatt ccgttgctcg atgtcgatca
     3301 gctgtttact ccggcagaac gcgatcagat cgcgtcgatt cgcaagagtt tttaaccacc
     3361 agaatcacaa caggaggttt tgtcatggcg aagtggaaat gtgtcgtgtg cgggtatgtg
     3421 tatgatccgg ctcagggtga ccctgacggt ggtatcaagc ccggtacgcc gttcgagcag
     3481 attcccgaca actgggtgtg cccgatttgc ggcgccggca aggatcagtt cgaaaagatc
     3541 gtctgaggct cgcccgcgca ttgccgcgga aacggccgct gcctgacccg atgcgggagt
     3601 ccggttcagc tcattctgag acgaaccgtt gaagcttgtg tcggtactgg tgtcggccgt
     3661 gcttcatttt ccgggaagta gggagtatcg ctcatgctgt cacgcttttc agtcaccata
     3721 ctggtcgttt ctgctctctg tgtcgtgttc atgccgtttt cgtcgccggc ccaggcggtc
     3781 acggactcca ccctcaccgc tggctctcca gaactgatcc acatctggcg cgatgactgc
     3841 ggcgtggcgc atgtcgttgc cagttccacc gcgggagcct actgggggtt cggctactgt
     3901 ctggcccgcg atcggctgct gcagctcgag ttgctgcggc gctcggtaca gggcaccttg
     3961 gccgaaatac tgggaaaatc ttttctggaa caggatgtgc tggcgcggcg tgacggcctg
     4021 tcgcagaccg aactgcagga aggactcgat cgcgccgatc cgctgttccg taccgcgttg
     4081 acctcatttg ctcgtggtat caatgcatca attgatgctg cttcgcgtag acgacagctg
     4141 gtacatccag catttcgctc ccttggtttg tcatttcgcc ccttcaccga acttgacata
     4201 ctcgagatct tcgcgggaac catggcttgc cggtataacg acttcacgca ggaactcgac
     4261 aatctcaagc tcctgaatga gctggtccgg cgactggggg cgcgcagtgc ctcgcgcatc
     4321 tttgatgatg tcatcccgtt tgttgatccg ggggtgtatg ccacgctggg gcgacgcgag
     4381 cactacatgc ccgcactgcc gggtgccgtg cgtttcgggc cccacccgca ggatctcccc
     4441 gaaggagaat cgccgacgct gcggcagcgg aagcgcaatc gcgaactgaa gaccatcggc
     4501 attccggaca agtcgggcag ctacgccgcg gtcctgtcac tgcgcgggca gggaaagccc
     4561 gaggcgcttc tgctgggtgg tccgcagatg ggatatttca agccctcggc ggtgtatgag
     4621 attggcttgc atacccccga cttcgatctg gtgggcgcga ccccggtcgg ctatttcgtc
     4681 atcctgttcg gtgccaaccg gcatctgggc ttcacggcca cggcgggcgt ggcgatgccg
     4741 tagccgaccc gcagcccggc caggccgt
//
LOCUS       DAORHD010000189         4751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18905_length_4751_cov_3.488501, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000189 DAORHD010000000
VERSION     DAORHD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18905_length_4751_cov_3.488501"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..829)
                     /locus_tag="PKO06_05380"
     CDS             complement(278..829)
                     /locus_tag="PKO06_05380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018283702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV69109.1"
                     /translation="MKVQIALFVLLWAATAAVFAQAANVPADVKLEKADHPLVKINTE
                     AGEIWLELFPETAPKHVESFLTLIGKGYYNGLKWHRVVPGFVIQGGCPKGDGTGGPGY
                     TLPAEFSARKHVKGTLAMARTSDPNSAGSQFYICLAEIPHLDNQYTVFGQTVKGMDVP
                     EKVKQGDSMKIEVIQNATPAKAK"
     gene            complement(995..1663)
                     /locus_tag="PKO06_05385"
     CDS             complement(995..1663)
                     /locus_tag="PKO06_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012637490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV69110.1"
                     /translation="MARLARLVISGVPHYITQRGNRAQEVFLRESDYRDYRSLMAEWC
                     GRQKIEIWAYCLMPNHIHLIAVPPDPDALQNAIGEAHRRYTRLVNAREGWCGHLWQGR
                     FGSFPMDDRSLLEAARSIELKPVRAGLARSPGEYPWSSAAAHLAGADDGLTVVKPLLS
                     RVDSWGGFLRQKCDEESDTRFQRHANTGKPLGSDEFIRRLELQTGRQLFPKKPGRKRK
                     ETAD"
     gene            complement(1900..3285)
                     /locus_tag="PKO06_05390"
     CDS             complement(1900..3285)
                     /locus_tag="PKO06_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNV69111.1"
                     /translation="MNRHSHHESSESSYSSDQYQKEVWPGSTLLAPVPIVMVTTQTPG
                     GKPNICTVAWAGIVCSNPPMLSISLQPSRHSFSHLMEQKEFVVNIPPRHLARETDMCG
                     VISGRDEDKFEKTHLTPVPAKDVLPPLIRECPVNLECRVTKVIELGSHHLFIAEIVRV
                     HVQKKLINETGRLAIEKARLLSYAHGHYYAIGIRIGRYGFSVRKHHPEENAPARKEKK
                     MKKKLEREASRAHDRPREARHDRYEEDAEHEERRPRRFERDDRGPREHRESRPHGHRG
                     HDRPPRREGGVWNTEPVERPRSDEGRDQGEDRPRRPRHHDDRTQGHDRPRRPRHHDDR
                     PQGDDRPRRPRPHDDQARGDDRPRRPRHHDDRPQGDDRPRRPRPHDDQARGDDRPRRP
                     RHHDDRPQGDDRPRRPRPHDEQSQSDARPRRDSGDRPRHHDRGEHSGRDRARERLREK
                     VGDRVRRRESR"
     gene            complement(3299..>4751)
                     /locus_tag="PKO06_05395"
     CDS             complement(3299..>4751)
                     /locus_tag="PKO06_05395"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69112.1"
                     /translation="FLQAESPAMAIEAIGVLEKQAPGYLKTVLPDVLQSRDLGLQGRA
                     LRSLLKLDPPQGVTFLKQQFASTDPVARAYAVMHCLAVPFDLVREPLFDMLAAEEDPR
                     ILNRAAYVLFLNPEEKTVWWLMDVAVGARPAKAKWCQNVVEAVLEAIRVSGIMAGSVS
                     DFVQKVRDFIRHRQVDTRLKSLLDELEHADPLHRYEAIEALRVYENEPEVRERFRRMY
                     LEERDEKVKNVLAAIFAQSPDRARLQKELIPAAFQKLKVPQQMEILGQIKNVEEFTFV
                     RDSLQALVRLHLDPYVQAEVVRLLGTYGDEKGELSPLSVALKSPEGVIVARAIEGLSR
                     IGPKSLLKELPKLIRSPVASVRTAALKAFFQEDKIQTLSVLREMAMSNSSQAKEQALI
                     CLAQINYSSSRELLLKFVQEEGNLALVKKAGVILKGNPDPDAIRFLHQQWRRTTGPKK
                     AALQEVMHDCIETAIAVEIIKGPPEAYLKTIES"
BASE COUNT          903 a         1422 c         1409 g         1017 t
ORIGIN      
        1 cctccacgag atcattctcg ttggtggctt caaagaacaa ctctgcggga ccgccgatat
       61 gaaaggtggt aaatgggccg agcggcacat gtttcttgat tcgtgaaccc agcggattca
      121 aggatatgga catgagaaca cctgtcacag gaggccttct gatgtccata tcccagcgga
      181 ttcaaggata tggacatcag aaggcctcct gtgacaggtg ttctcttctg atatcggccg
      241 aaatgccgcc cggcaccatc attttctgca gatcgtccta cttggctttc gccggagtgg
      301 cattctgaat gacttcgatc ttcatcgaat cgccctgctt caccttctcg ggcacatcca
      361 tgcctttcac ggtctggccg aacacggtgt actgattgtc caggtgcgga atttctgcga
      421 ggcagatgta gaactggcta cctgccgagt tcgggtcgct ggtgcgcgcc atggcgagag
      481 ttcccttcac gtgcttgcgc gcgctgaact ctgcgggcag ggtgtagccg gggccaccgg
      541 taccgtcgcc cttggggcag ccgccctgaa tgacgaaacc cggaaccacg cgatgccatt
      601 tgagaccgtt ataataccct ttaccaatca gcgtcaggaa gctttccacg tgcttggggg
      661 cagtttccgg gaacagctcc agccagatct caccggcttc ggtgttgatc ttgaccagcg
      721 gatggtccgc tttttccagc ttcacgtcag ccggcacatt ggcggcctgc gcgaacacgg
      781 ccgccgtcgc ggcccacaac agcacgaaca gagcaatctg aactttcatg aacttcctcc
      841 tttggaatat gaggcgccta ttctagcccc agttgccagc tctcgcaagc ccgttccggg
      901 gaaaaaccga atcagacgtg cgcttgaact cgagcacctg gcgcagtgtg tcgcgagcaa
      961 cctggggcag aaacaatcgc acgcgacgct ctgttcagtc agcggtttct ttgcgcttgc
     1021 ggccgggttt cttcggaaac agctgtcgcc ccgtctgcag ttcgaggcga cgaatgaact
     1081 catcgctgcc gagtggtttt ccggtgttgg catggcgctg aaaccgggtg tcagattctt
     1141 cgtcgcattt ctggcgcaga aacccgcccc aggaatcaac ccgcgacagc agcggcttca
     1201 cgacggtcaa cccatcatca gcgcccgcca ggtgagcagc agcagaactc cacgggtatt
     1261 cccccggcga cctggccaga ccagcacgca cgggcttgag ctcgatggag cgagccgctt
     1321 cgagcaggga tcgatcatcc atcggaaacg acccgaatcg cccctgccac aggtgcccgc
     1381 accacccttc ccgtgcattc acgagacgag tgtaccggcg atgagcttct ccaatggcgt
     1441 tctgaagagc atctgggtct ggtggaaccg cgatgaggtg aatgtggttc ggcatcaggc
     1501 aataggccca gatctcgatc ttctgtctgc cgcaccactc ggccatcagc gagcgatagt
     1561 cgcggtagtc agactcacgc agaaacactt cctgagcgcg gtttccgcgc tgggtgatgt
     1621 aatgtggaac tcccgaaatg acaaggcgtg ccagacgtgc cataccgcga ttctatgcag
     1681 atcgacaaaa aaacgcaaga tataagtata ctgtcgccgc gttctcgtcg attccaagaa
     1741 attctgtcgc ctcatcacca ctctgcggga aaacagaaca agaccccgtt ttctcgaggt
     1801 cttgtcagaa tgtctcagct acgatcgttc tgccgttcag acagaacaag caaacccagc
     1861 tcccggcacg gcgggggaac gtcttctccc tgaatcgact cagcgcgact cgcgacgccg
     1921 cacgcgatcg ccgacttttt cgcgcaggcg ttcgcgggcc cggtcgcggc cgctgtgctc
     1981 gcctcgatcg tggtgacgcg ggcggtcacc ggaatcgcgg cgcggccgtg cgtcactctg
     2041 cgattgctca tcatgcggtc gagggcggcg gggccgatcg tcgccctgag gtcgatcgtc
     2101 atggtgacga ggccggcgtg ggcggtcatc gccccgagcc tgatcatcgt gcggtcgggg
     2161 gcggcggggc cgatcatcgc cttgaggtcg atcgtcatgg tgacgaggcc ggcgtgggcg
     2221 gtcatcgcct cgagcctgat catcgtgcgg tcgcggccgg cgtggccgat catcgccctg
     2281 aggtcgatcg tcatggtgac gaggccggcg tgggcggtcg tgaccctgcg tgcggtcgtc
     2341 gtgatggcgc gggcgacgcg ggcgatcttc gccctgatcg cggccttcgt ccgatcgcgg
     2401 gcgttcaacg ggttccgtgt tccagacgcc gccttcgcgg cgcggcgggc ggtcgtgccc
     2461 gcggtggcca tgcggtcgcg actcacgatg ttcacgcgga ccgcggtcgt cgcgttcgaa
     2521 acgacgcggt cgccgttcct catgctcggc atcttcttcg taccggtcat gtctggcttc
     2581 tcttggcctg tcatgagcgc gcgaagcttc tcgttcgagc ttcttcttca tctttttttc
     2641 ttttcgggcg ggcgcgtttt cctcaggatg atgtttccgc acgctgaatc cgtaccggcc
     2701 gatgcgaatg ccgatcgcgt agtaatggcc gtgagcataa ctgagaagtc tggctttttc
     2761 gatcgccagt cgaccggttt cgttgatcag tttcttctgc acatgcacgc gcacgatctc
     2821 agcgatgaac aggtgatgcg agcccagttc gatcaccttc gtcacgcgac actcgagatt
     2881 caccggacat tcgcgaatca gcggcggcag tacgtctttg gccggaaccg gggtcagatg
     2941 cgttttttcg aacttgtcct catcgcgccc cgagatgacg ccgcacatgt cggtttcgcg
     3001 cgccagatga cgcggcggaa tgttgaccac gaactccttc tgttccatca gatgcgaaaa
     3061 cgaatgccgc gacggctgca acgagatcga cagcatgggc gggttgctgc agacgatgcc
     3121 ggcccaggca acggtacaga tgttgggctt gccgcctggt gtctgcgtgg tgaccatgac
     3181 gatgggcacg ggagccagca gagtggaacc aggccagact tctttctgat actggtctga
     3241 agagtacgac gactcggacg attcatggtg agaatgacgg ttcatgttgg ctccgggttc
     3301 aggattcgat cgttttgaga taggcctctg gaggcccttt gataatttcc acggcaatgg
     3361 cggtctcgat acagtcgtgc atgacctcct gcagagccgc ttttttcggc ccggtcgtgc
     3421 gacgccactg ttgatgcagg aagcgaatgg cgtctggatc aggatttccc ttgaggatca
     3481 cgcccgcctt tttcaccagg gccaggttcc cttcttcctg gacgaacttc agcagaagtt
     3541 cgcgcgagga cgagtagttg atctgagcca ggcagatcag cgcctgttct ttggcctgcg
     3601 agctgttgga catggccatc tcgcgcaaca ccgacagcgt ctgaatcttg tcttcctgga
     3661 aaaaggcctt cagcgccgcc gtgcgcaccg acgcgaccgg cgagcggatt agcttgggca
     3721 gttctttcag caggcttttg ggaccgatac gcgacaaccc ttcgatcgcc cgtgccacga
     3781 tgactccttc aggactcttg agtgcaaccg acaacgggct cagttcgcct ttttcgtcgc
     3841 cgtaggttcc gagcaggcgc acgacctcag cctgaacgta cgggtcgaga tgcagtcgaa
     3901 ccagcgcctg aaggctgtca cggacgaacg taaactcttc gacgttcttg atctgcccga
     3961 gaatttccat ttgctgcggc actttcagtt tctgaaacgc agcgggaatc agctctttct
     4021 gcagccgcgc acggtcggga ctctgggcga atatggccgc gagcacgttc ttgaccttct
     4081 cgtcgcgctc ttcaagatac atgcggcgga aacgctcacg gacttccggt tcgttctcgt
     4141 agacgcgcaa tgcttcgatg gcttcgtacc gatgcagcgg atcagcatgt tcgagttcgt
     4201 cgaggagcga tttcagacgg gtgtcgacct gacgatgcct gatgaaatca cgcaccttct
     4261 gaacgaaatc gctgaccgag ccggccatga tacccgacac gcgaatggct tcgagcacgg
     4321 cttcgacgac attctgacac cacttcgcct tggcggggcg agccccgaca gcgacatcca
     4381 tcagccacca gacggtcttt tcttcggggt tgaggaacag cacgtaggcg gcccgattga
     4441 gaatgcgagg atcttcttct gccgcgagca tgtcgaacag cggctcacgt accagatcaa
     4501 atgggacggc gagacagtgc atgaccgcat aagcccgcgc gaccgggtcg gtagaggcga
     4561 actgctgttt gagaaatgta acgccctgcg gcggatcgag tttcaacagc gaacgcaacg
     4621 cgcggccctg caagcccaga tcgcgactct gcagcacgtc gggcagaacc gtcttcagat
     4681 agccgggagc ctgcttctca agcaccccga tcgcctcgat ggccatggcc ggcgattcgg
     4741 cctgtagaaa c
//
LOCUS       DAORHD010000190         4737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_18984_length_4737_cov_4.153994, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000190 DAORHD010000000
VERSION     DAORHD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18984_length_4737_cov_4.153994"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1534)
                     /gene="dnaA"
                     /locus_tag="PKO06_05400"
     CDS             complement(152..1534)
                     /gene="dnaA"
                     /locus_tag="PKO06_05400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNV69113.1"
                     /translation="MKEIWSKISEEIRSQVNQTNWDIFQSSIFPAKVEGNQLVLEAPN
                     EFCKDWVERRYRSMIQKILHEANPGLSCVIVIKPRLGDTAPGKPYQLQLGEVEPQAGF
                     VPKATTVKPSAANKQDATLNGKYSFETFVVGKSNQFAHAVCQAVAKSPGTQYNPVFMY
                     GGVGLGKTHLLQAVGHEVLRLHPKAKIAYLSSESFTNEFIDALMNKKTNDFRSKYRKK
                     DLLLIDDIQFLAGKDAVVEEFFHTFNDLFQNKKQIILTSDVPPKKMKDMQERLISRFE
                     MGVVADIQAPDIETRVAILKSCMESAHLKASEDVLMYLAENVKNNIRELEGAFTRVIA
                     YSSVVQKPVDRHLVDQVLKDLFREGGSGKITIPWIQKKVAEFYDIPETEMTSKRRAAN
                     LVLPRQVAMKLSQLMTSSSLSQIGDAFGGRDHTTVMHSCEKIGKMQKSNTSFAEEFDR
                     VSRYVKPSAS"
     gene            complement(1531..1866)
                     /locus_tag="PKO06_05405"
     CDS             complement(1531..1866)
                     /locus_tag="PKO06_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69114.1"
                     /translation="METVENPVKSCIRATIPTVRLGENHFTCSRFSDLFCFPQVIHDL
                     SPETPVENPVGYQKLVYTVGKKPLASKQFLCYASETSEDSPNNDHKLIYEGDALSRKK
                     TSVQHEGGK"
     gene            2051..2602
                     /gene="accB"
                     /locus_tag="PKO06_05410"
     CDS             2051..2602
                     /gene="accB"
                     /locus_tag="PKO06_05410"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNV69115.1"
                     /translation="MPKTARVQAKKAVATENPEQTISEVVNLIKLMGETGLAELRMES
                     PGLKVSLKKESTPSFVGFQTPSTLAMPSMVVPQATASASTKTTPTEPAKTAPKIDENA
                     KYHQVCSPMAGTFYRQPSPNSPPYVKEGDQVKNGQPLCIIEAMKLMNEIKSEVTGKII
                     KIVAENAKPVDKGSILFLVDKNS"
     gene            2614..3987
                     /gene="lpdA"
                     /locus_tag="PKO06_05415"
     CDS             2614..3987
                     /gene="lpdA"
                     /locus_tag="PKO06_05415"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNV69116.1"
                     /translation="MTQSYDLIIIGAGPGGYPLALRCATQGMKVAIIERDNVGGTCLN
                     WGCIPTKTLLASAKLLSHLKHASDFGLRAETIGFDWAKVQERKEQVVKRLRQGIHKLL
                     DNANVKLFTGTGYLKPGNVVTVEGLDEELSASRVCLAVGSVPAIPSVFPADRTSCWSS
                     DEALNATTIPDSLLVVGGGVIGLELGQVYSEFGCRVTIVEMIDQILPGLDSATAKRLL
                     PVFKKTGIEILTGQKVEKMQVQSDSSVETVISGQTRTFSRALLAIGRRPNLKVFEKLA
                     QPPEQIGPFLKVGTDYQTSIPGVYAIGDCIHGPMLAHKASYDALTLSRQWAGEALVPN
                     YDTVPSCVYTYPEIAWVGRSEDQLKKDQVEYKVGRSQFSANGKALAMGEADGQIKTMI
                     GTEGKILGTVMWGPETSNLIMEGTILQGLGLLGIDVAQRVIHPHPTLAEAYLDALENG
                     LGSGVHG"
     gene            4000..>4737
                     /locus_tag="PKO06_05420"
     CDS             4000..>4737
                     /locus_tag="PKO06_05420"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69117.1"
                     /translation="MPTVGFLQLPLWHEALRAQLRQFGQEELLRAPVLNAEDTGDGWK
                     SLSTEEFFRFVQVGLHFEAVRPIAQITDILITSDANLALKRILEFPTGTTGVRGALGG
                     LSPLLDLFNRDCFQDRPRRMLCIADGVTNFVLNEMEHQERTLENAVQEAQWQNIAPGN
                     PTRHLHGLVARERLALLATVLFGIKLSPDQILVEGIAKLEQKDIQLARSLDYSLRLLG
                     AIERRENRIEAWVRPCLIPCRYLLSQVR"
BASE COUNT         1131 a         1221 c         1216 g         1169 t
ORIGIN      
        1 gactagaccc catcgaatag aggctgggag tatctgtcgc ctcttcacct gtgaagcaac
       61 acccgaacct ctggacgaag gtccgaagat gatactccaa ttctctctct ctttcaagag
      121 gagaaccaaa gaaaacctga tttgaagcgg ttcaggatgc tgacggcttg acgtatcgac
      181 tgactcgatc aaactcttca gcaaacgacg tattcgattt ctgcatcttg cctatctttt
      241 cacaagaatg catgaccgtg gtgtgatctc gcccgccaaa cgcatcgccg atttgactga
      301 gtgatgacga agtcatcagt tgcgagagtt tcatcgccac ctgtcgaggt agaaccagat
      361 ttgccgcccg gcgcttgctg gtcatttccg tttccggaat gtcgtaaaac tcggcaacct
      421 tcttctgaat ccaggggatg gtgatctttc cggagccgcc ttcgcgaaac agatctttca
      481 gaacctgatc gaccagatga cggtcgaccg gcttctgaac aacgctcgaa tacgcgatca
      541 cccgagtaaa ggccccttcg agttctcgaa tattgttctt cacgttttct gccaggtaca
      601 tgagcacgtc ttctgaagcc ttgagatgcg ctgattccat gcagcttttg agaatcgcca
      661 cccgtgtctc gatatctggg gcctgaatat cggccacgac tcccatttcg aaccgtgaaa
      721 tcagccgttc ctgcatgtct ttcattttct tgggtgggac atctgacgtg agaatgatct
      781 gcttcttgtt ctgaaacaaa tcgttgaatg tgtgaaagaa ctcctcaaca acggcatctt
      841 tgccggccag aaactgaatg tcgtcgatca gcagcaggtc cttcttccgg tactttgacc
      901 gaaagtcatt agtctttttg ttcatgagag catcaatgaa ctcattcgta aacgattcag
      961 aagagagata cgcaattttg gctttgggat gcaatcgcag gacttcatgt ccgacagcct
     1021 gcagcaggtg ggtttttcca agaccgacac caccatacat gaagaccggg ttgtattgcg
     1081 tgccaggtga cttggcaacc gcctggcaca ctgcatgcgc aaactgattg ctcttgccaa
     1141 ccacgaacgt ctcaaaggag tatttgccgt tcagagtcgc atcctgcttg ttcgcggcac
     1201 tcggcttcac cgtggttgcc tttggcacaa accccgcctg gggctcaact tcaccaagtt
     1261 gcaactggta cggttttccg ggagcagtgt caccaagacg aggtttgatc acgatgacgc
     1321 acgagaggcc gggattggct tcatgcagga ttttctgaat catgcttcga tatcggcgtt
     1381 caacccagtc tttgcagaat tcgttcgggg cttcgagaac cagttgattt ccttcgacct
     1441 tcgccggaaa gatcgagctt tgaaaaatgt cccagtttgt ttggttgacc tgtgatcgta
     1501 tctcttctga aatcttgctc cagatttcct tcatttgcca ccctcgtgtt gtacactcgt
     1561 cttttttcga gaaagcgcat ccccttcgta gatcagcttg tgatcattgt ttggggaatc
     1621 ttcgctggtt tccgaagcat agcagagaaa ctgtttcgag gcaagaggct ttttaccaac
     1681 tgtgtacacc aatttctgat agccgaccgg gttttccacg ggtgtttctg gggaaaggtc
     1741 gtggataacc tggggaaaac aaaacaggtc ggaaaaccgg gaacaagtga agtggttttc
     1801 cccaagtctt acagtcggga ttgtcgctct aatacaagat ttcaccgggt tttccaccgt
     1861 ttccacaccc cttactgctt ctactgtttt tctttcttta gtgagttgta cactaataat
     1921 tcaggcttgt ggatttcaat cgccagctca ttatgtaccg tctcttggaa agtctgaatt
     1981 catcgtggtt tcattgacgc tcccagaccc atctggtaga atgcgccatc tctcgacttg
     2041 gaggatcacc atgcccaaga cagcccgcgt tcaggcaaag aaagcagttg ctaccgagaa
     2101 ccccgaacag accatctctg aagtggtcaa tctcatcaaa ctcatgggag aaaccgggtt
     2161 agctgagttg cgcatggaat cccccggact gaaagtttcg ctgaaaaagg aatcgacacc
     2221 ttcattcgtt ggttttcaaa ctccgtcaac cctggccatg ccttcgatgg tggtgcctca
     2281 ggcgactgct tccgcatcca cgaagacaac tccgacagag ccggccaaga cggcacccaa
     2341 aatcgacgag aacgcgaaat accatcaggt gtgttccccc atggccggca cgttttatcg
     2401 acaaccatcg ccgaattctc cgccttatgt aaaagaagga gaccaggtga agaatgggca
     2461 acctctgtgc atcattgaag ccatgaagct gatgaacgaa atcaaatcag aagtgactgg
     2521 aaaaatcatc aagattgtgg ctgaaaatgc caagccagtc gacaaaggct cgatactttt
     2581 cctggtcgac aagaactcct gagttcagta acaatgacgc aatcatatga tttgatcatc
     2641 attggagccg gtcccggcgg gtatcctctc gcgcttcgct gtgccactca gggaatgaaa
     2701 gttgccatca tcgaacgaga caatgttggt ggcacctgtt tgaattgggg ctgtatcccg
     2761 actaaaacgc tgctggcttc agccaagctg ctttcgcatt tgaagcatgc gtctgacttt
     2821 gggctgcggg ccgaaaccat cgggtttgac tgggcaaaag tgcaggaacg gaaagaacag
     2881 gtcgtgaagc ggcttcgcca gggaattcac aagcttctgg acaatgccaa cgtgaagctc
     2941 ttcacgggaa cgggatatct gaaaccaggt aacgtggtca ccgtcgaagg acttgatgaa
     3001 gaactgagcg catcacgagt ctgtcttgcc gttggttcgg ttcctgccat cccgtctgtg
     3061 tttcccgccg atcgcacgtc atgctggagc agtgatgaag ctttgaacgc gacgaccatt
     3121 cctgattcac tgctggttgt gggcggtgga gtcatcggac tggaactggg acaggtttac
     3181 agtgaatttg gttgccgggt gaccatcgtt gagatgattg atcagatcct tccaggctta
     3241 gattccgcga ccgcaaagcg gctgctgccg gttttcaaaa aaaccggaat cgaaattctc
     3301 acgggtcaga aagttgagaa gatgcaggtt cagagcgatt catcggtaga aaccgtgatc
     3361 tctggacaaa cgcggacatt ctctcgagca cttctcgcta ttgggcgacg ccccaacctg
     3421 aaagttttcg agaaactcgc ccaacctcct gagcagattg gccccttcct gaaagtcgga
     3481 acggactatc agacgagcat tcccggggtg tatgccattg gcgactgtat tcacggacca
     3541 atgctggctc acaaggcttc gtatgatgct ttgacactct cgcgacagtg ggcgggcgaa
     3601 gcgctggttc ccaattacga tacggttccg tcgtgcgtgt atacgtaccc ggagatcgcc
     3661 tgggttggac gctcagaaga ccaactgaaa aaggatcagg tggaatacaa ggttggacgc
     3721 agtcagtttt ccgcgaacgg aaaagcgttg gccatgggcg aagccgatgg tcagatcaaa
     3781 accatgatcg gcacggaagg gaaaattctg ggcacggtca tgtggggccc ggaaaccagc
     3841 aatctgatca tggaaggcac gattctgcag ggattggggc ttctcggcat cgatgtcgct
     3901 caacgggtca tccatccgca cccgaccttg gccgaagcgt atctcgatgc cttggaaaac
     3961 ggcctcggca gcggcgttca cggataaggc tcagcgggca tgccgactgt cggatttctg
     4021 caactgccct tatggcatga agcgttgcgc gcgcaactgc gtcagtttgg ccaggaagag
     4081 ttgttgcgtg ctccggttct caacgccgaa gacacgggtg atgggtggaa gagcctttcg
     4141 acggaagagt tttttcgctt tgttcaagtc ggcctgcatt ttgaggcggt tcgcccgatc
     4201 gcacaaatca ctgacattct cattacgtca gatgcaaatc tcgctctgaa aagaattcta
     4261 gaatttccaa ctgggacaac tggagttcgt ggcgctcttg gaggcctatc gccacttctc
     4321 gatctcttca accgcgactg ctttcaggat cgcccccggc gcatgctctg cattgccgat
     4381 ggagtgacga atttcgttct gaacgagatg gaacaccagg agcgaacgct tgaaaatgcc
     4441 gtgcaggaag cgcaatggca gaacatcgct cccggcaacc cgacacgaca tctgcatggt
     4501 ctcgtggcgc gcgaacgact ggctcttttg gctactgtcc tatttggtat caagctatct
     4561 cctgatcaaa tacttgttga aggtattgca aaactggaac agaaagacat tcagttagcc
     4621 agatcactgg actattccct acgactgctg ggagccatcg aacgacgtga aaacaggatc
     4681 gaagcctggg tcagaccatg tttgatcccc tgccgatatc tgctctcaca ggttcgc
//
LOCUS       DAORHD010000191         4728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19062_length_4728_cov_3.870319, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000191 DAORHD010000000
VERSION     DAORHD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19062_length_4728_cov_3.870319"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKO06_05425"
     CDS             complement(<1..475)
                     /locus_tag="PKO06_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNV69118.1"
                     /translation="MELIKNELKQTVEDCKKRARDHGLSFRDETLEFIVTNEDMIKLS
                     PKGMIPTLYDYWVHDIYVLQDKGKYEFYPHNPYETVINTRPPLSFYNDNNPDWLNVMI
                     FYHVIGHIDFFQNNVFFAKTWLDDFLSKAAADAQVINDLRQEHGRWVDYVIEFARG"
     gene            complement(484..1866)
                     /locus_tag="PKO06_05430"
     CDS             complement(484..1866)
                     /locus_tag="PKO06_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNV69119.1"
                     /translation="MKQPPILSLRSIPLPELLRREDLSPEIKASIRRDLDGTLKEAAT
                     GSRSGGVPAQAPGDHAERPFDDGILFNPHHDPTVRLQTLDELLDRDEQREKDGFPKKV
                     ILGKIASPRGQKSLIVPTVTEEKFYHDNRPKSPGQGGSEGGSGDGEEGEVIGESPIRE
                     EQQGQGKGQGGQDGGEGHDLDDSRAFEIGKALTEKFQLPNIQEKGKKYSLFRYTYELT
                     DINRKFGQILEKKRSLKNILKTNLQLGTFDPDNISFDKLIIGPNDLVYRIVSPEKEYE
                     SQALVFFVRDYSGSMYGETTEVVLNQHVLVYSWLVYQYQERVDKRFILHDTDAKEVQN
                     FVTYYKLNNGGGTNIASAYKLINQIVEKESLARDYNIYVFQGTDGDDWDSDGKEALPE
                     LEKMLTYVNRMGITVVKGRHSSSETSVEQYLKKSNLLEKHKNLLRLSTLDETQKNQDA
                     LIKSIRELVE"
     gene            complement(1879..3924)
                     /locus_tag="PKO06_05435"
     CDS             complement(1879..3924)
                     /locus_tag="PKO06_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HNV69120.1"
                     /translation="MAKTLPEYLKALREGKHRFESVFRSVARMILDDPAKVEKVTVNG
                     KQTYDYKVFREGKKPVVGMFDLINSFVSYVKDAAENGSSREMAFVLVGEPGNGKTFFV
                     DRVCDLYREYLTKPQNAKWTFQFRNLHQLGKYGKLTTIESQSYEDPVILAMNIDHDRS
                     VCRQFLGKLGFDDKTIDRFYQDYRPLGACSQYILEDIRAFTGNDLNKMLSDFIDVVPV
                     PLSGGLGTITGKYQAKDKITSSGADLTGEESVQRLLYLADASNPYRYDLRRGVLARVA
                     GGGIHFADELFKNKTDLVQIYLGVIQNREIELNAYKWPMDTLIIATSNIVEYQKFTEN
                     REQAPIFDRCKIFYVPHNTDYRLQEKLTGFAIGSKTKTTVLGKGLHEDPNLNYGISVS
                     MVLTRLPQDERLAPREMLQLAAGEVAGEKSIKTLAEIIDELSKRQKINERFGHSGIGQ
                     RGLGRSIQHLLSLNETQEDECMFAGNAFDSIRRETYDNIIDKKEQEWCLKVLEVAEGL
                     YKEKIKTEIFNAYMNDPEAIKKEVMNYVNMIVVSSDDDGPDKVVRIREGNVERPLKID
                     ETFINGVEQRLGLNTREQREQHRAQIRAIYARRVTQDPNYCFMDNEKLKKAVTEYRLN
                     TDISGAGSLAGALANPTNEENKKLFDRLFSVMIDKLGYCKTCARKTLEFYCKPDSNK"
     gene            complement(4437..>4728)
                     /locus_tag="PKO06_05440"
     CDS             complement(4437..>4728)
                     /locus_tag="PKO06_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV69121.1"
                     /translation="HISTISIAELYTGIRDGVERDSLDAAIEACHVVDVTGVIARKAG
                     LWQRTYGKSHGVHLPDALIAATVQEWSLTLATLNRRHFPMLADIVTPYGKSR"
BASE COUNT          866 a         1305 c         1414 g         1143 t
ORIGIN      
        1 ccccgcgcgc gaactcgatc acgtagtcga cccagcgacc gtgttcctga cgcagatcat
       61 tgatcacctg ggcgtcggcg gccgccttgc tgagaaagtc gtcgagccag gtcttggcga
      121 aaaagacgtt gttctggaaa aagtcgatgt gtccgatgac gtgatagaag atcatcacgt
      181 tcagccagtc ggggttgttg tcgttgtaga aagacagcgg cggccgtgtg ttgatgaccg
      241 tctcgtaggg gttgtggggg tagaactcgt acttcccttt gtcctgcagg acgtagatat
      301 cgtgcaccca gtagtcgtac agggtcggaa tcatgccttt cggactgagc ttgatcatgt
      361 cctcgttcgt cacgatgaat tcgagcgtct catcgcggaa ggacaaccca tgatcgcggg
      421 cgcgcttctt gcaatcttcc accgtctgct tgagctcgtt cttgatcagt tccatggtat
      481 gtcctactcc accagctcgc gaatgctctt gatcagggca tcctggttct tctgggtttc
      541 atcgagagtc gaaagccgca gcagattctt gtgcttttcg agcaggttcg atttcttcag
      601 atactgctcg accgatgtct cagagcttga atgacgaccc ttgaccacgg tgatgcccat
      661 gcgattgacg taggtgagca tcttctcgag ttccggcagg gcttctttgc cgtcgctgtc
      721 ccagtcgtcg ccgtcagttc cctgaaagac gtagatgttg tagtctcgtg ccaggctctc
      781 tttctcgaca atctggttga tcagcttgta cgccgaagcg atgttcgtgc cgccgccgtt
      841 gttcagcttg taataggtga cgaagttctg cacttccttg gcgtcggtat cgtgcagaat
      901 aaagcgcttg tcgacccgtt cctgatactg gtagaccagc cacgagtaga ccagcacatg
      961 ctggttgagc acgacctcgg tggtttcgcc atacattgag ccgctgtaat cgcgaacgaa
     1021 gaaaacaagc gcctgggact catactcctt ttcgggcgac acgatgcggt agaccagatc
     1081 gttcgggccg atgatcagct tgtcgaacga gatgttgtcg gggtcgaagg tgccgagctg
     1141 cagattggtc ttgagaatgt tcttcaggct gcgcttcttc tcgagaatct ggccgaactt
     1201 gcggttgatg tcggtcagtt cgtaggtgta gcgaaagagc gaatactttt tgcccttctc
     1261 ctgaatgttc ggcagctgaa acttttcggt gagggccttg ccgatctcga aggcgcggga
     1321 atcatcgagg tcgtggccct cgccaccgtc ctggccgccc tgccctttgc cctgcccctg
     1381 ctgctcttcg cgaatcggcg attccccgat gacttcgcct tcttcgccat cgcccgagcc
     1441 gccttccgag ccgccctgtc cagggctttt cgggcggttg tcgtggtaga acttttcttc
     1501 ggtgacggtg ggcacgatca gcgacttctg gccgcgcggg ctggcgatct tgcccagaat
     1561 cacctttttg gggaatccat ctttctcgcg ctgctcatcc cggtcgagca gttcgtccag
     1621 ggtctgcagg cgcaccgtgg ggtcgtgatg cgggttgaag agtataccgt catcgaacgg
     1681 ccgttcggcg tggtcgccgg gagcctgcgc gggcactccg ccggagcgag aaccggtcgc
     1741 ggcttccttc agcgtaccgt cgagatcacg tcgaatgctg gccttgatct ccggagacag
     1801 atcctcccgc cggaggagct ccggcagggg aatggaccgc aaagacagaa tcggtggttg
     1861 cttcatggca gcaacctgtt acttgttgct gtcgggcttg cagtaaaact cgagggtttt
     1921 gcgggcgcag gtcttgcagt agcccagctt gtcgatcatc accgagaaca ggcggtcgaa
     1981 caattttttg ttctcttcgt tggtcgggtt tgccagggct ccggcgaggc tgccggcgcc
     2041 ggaaatatcg gtattcaacc ggtactcggt gacggccttc ttgagctttt cgttgtccat
     2101 gaagcagtag ttcgggtctt gcgtgacgcg gcgggcgtag atggcacgaa tctgggcgcg
     2161 atgttgttcg cgctgttcgc gcgtgttcag gccgagccgt tgttcgacgc cattgatgaa
     2221 cgtttcgtcg atcttgagcg ggcgctcgac attaccttcg cgaatgcgta ccactttgtc
     2281 ggggccgtca tcgtcgctgg aaacgacgat catgttgacg tagttcatga cctctttctt
     2341 gatcgcctcg gggtcgttca tgtaggcgtt gaagatctcg gtcttgatct tttctttgta
     2401 caaaccctcg gcgacctcga gcaccttcag acaccattcc tgttctttct tgtcgatgat
     2461 gttgtcgtag gtttcgcggc gaatgctgtc aaaggcgttg ccggcgaaca tgcactcgtc
     2521 ttcctgagtc tcgttgaggc tgagcagatg ctgaatcgag cggccgagcc cgcgttggcc
     2581 gatgcccgag tgtccgaagc gttcgttgat cttctggcgc ttgctgagtt cgtcgatgat
     2641 ctcggcgagg gtcttgatgc tcttctctcc cgcgacttcg ccggccgcca actgcagcat
     2701 ctcgcgcggg gccagacgtt cgtcctgtgg caggcgggtg agaaccatcg agacggaaat
     2761 gccgtagttg aggttcgggt cttcatgcag ccccttgcca agtacggtgg tcttggtctt
     2821 tgagccgatg gcgaatccgg tcagcttttc ctgcaggcga taatcggtgt tgtgcggtac
     2881 gtagaagatc ttgcaccggt caaagatggg cgcctgctcg cgattctcgg taaacttctg
     2941 gtactcgacg atattcgagg tcgcgatgat cagggtgtcc atcggccact tgtacgcgtt
     3001 cagctcgatc tcgcggttct gaatgacgcc gaggtagatc tgcacgaggt cggtcttgtt
     3061 cttgaacagc tcatcggcga agtgaatgcc gccaccggcc acgcgagcca gcacgccgcg
     3121 gcgcaggtcg tagcgatagg ggttcgatgc gtcggcaagg tagagcaggc gctgtaccga
     3181 ttcttcgccg gtgagatcgg ctccggaaga ggtaatcttg tctttcgcct ggtactttcc
     3241 ggtaatcgtg cccagaccgc cgctgagcgg aaccggcacc acatcaatga agtcgctgag
     3301 catcttgttg agatcgttgc cggtgaaggc acgaatatct tcgagaatgt actgggagca
     3361 ggcaccgagt gggcggtagt cctggtagaa gcggtcgatg gtcttgtcgt cgaaaccgag
     3421 cttgcccagg aactggcggc agacgctgcg gtcatgatcg atgttcatgg ccagaatgac
     3481 cgggtcttcg tatgactgcg actcgatggt ggtcagcttg ccgtacttgc cgagctgatg
     3541 caggttgcgg aactgaaagg tccacttcgc gttctgcggc ttggtgagat actcgcggta
     3601 cagatcgcag acgcgatcga cgaagaaggt cttgccgttg cccggctcac ccacgaggac
     3661 gaaagccatt tcccgcgacg agccgttttc ggcagcgtct ttgacatagg aaacgaagct
     3721 gttgatcagg tcaaacatgc ccacgaccgg tttcttgcct tcgcggaaga ctttgtagtc
     3781 gtaggtctgc ttgccgttga ccgtgacttt ctccaccttc gcggggtcat cgagaatcat
     3841 ccgcgccaca gagcggaaca cgctttcgaa ccggtgcttt ccctcgcgca acgccttcag
     3901 atactcgggc aacgtcttgg ccatactctc tccctcgtca ttaagatttt tcccactata
     3961 gcactttttt cggggcttgg gtacccccct gaaaccagct tcagcaaaga agaagccgat
     4021 caaccaccag aagtgcggcc aggtcggctt ccgggttgaa accaacactt cgccacttcc
     4081 cctgtccgac ccctgcgaag gtctccccgc agcggggacg aaggaagcaa cgaagggagt
     4141 cggtgctgag ctaccttatg gccggtggac ggagatggac cagaggcgca cgtggggctc
     4201 gacgcgggtg gattcgcgtt gattatcagc ggtttcctgc ggaacgccgt tcggcgcgat
     4261 gacttctctg ttcggcttcc caacctgcac aggcaagaag aatgtcgcgg ggaatcggcc
     4321 gtgcgccggt accatacgcg ctgatcgttc gaggagtgag gccggccaaa gaccgtgagc
     4381 ctgcatggtt tcgagataga tgatccttcg cgcatcgcgc aagaaataca ccgtattcag
     4441 cggctcttcc catacggcgt gacgatgtcg gccaacatcg ggaaatgtcg gcggttaagc
     4501 gtggccagcg tgagagacca ttcctgcacc gttgcggcaa tcaaagcatc cggcaggtga
     4561 acgccgtggc tcttgccgta tgttcgttgc cacaagcccg ccttcctggc gattactcct
     4621 gtgacatcca caacgtgaca ggcttcgatg gcggcatcca ggctgtcacg ttcaactccg
     4681 tcgcgaatac cggtatacaa ttccgcgatc gagatagtgg aaatgtgg
//
LOCUS       DAORHD010000192         4710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19157_length_4710_cov_3.528034, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000192 DAORHD010000000
VERSION     DAORHD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19157_length_4710_cov_3.528034"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKO06_05445"
     CDS             <1..1639
                     /locus_tag="PKO06_05445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69122.1"
                     /translation="AQMQRLLIHYLLIGVCIFLATAVIGLQAISSVSRHLQTLTQSIQ
                     DLVKCEFAPKPEAQNDLQQISESYRDEVGYLARAFLSMQRALQSYLQNLARTTAQKEK
                     IQSELRIAHNIQMSMVPHRFPRPEETGGIMLHAVLEPAREVGGDFYDFFPLTSSGDRS
                     AAFDHASSLPVPDRLAFAIGDVSGKGMPAALFMAMVKTLFRATAAACSDPARILSKLN
                     DEMSRDNATSMFTTMQVGIIDLRTRMVTLASGGHNYPLLISAEGKVREMKLQNALPIG
                     VYESVSYTNQEFTLQEGDTLVLFTDGITEAMNEKLEEFSDERFQAILEGKQNTGLEQL
                     STNVYQEVRRYAGTQDPSDDATLLLIRVFKPQQKIATGGPTLSETAAFGLAPTLPQPA
                     APSNSSHRQTVEIRDSADLKQIQDWLDREAPAWGLSQPDTHDLHLILDELIENVLRHG
                     YANTEPAQIRLEVTVAPESIALCLRDTGRAFNPLLQQSPDTTSAVATRAVGGWGIHLV
                     KQYSDTLSYERQNDQNVVRLSIRRRALKPQEGVTTDA"
     gene            1636..1974
                     /locus_tag="PKO06_05450"
     CDS             1636..1974
                     /locus_tag="PKO06_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV69123.1"
                     /translation="MNLQITQEQHGQVNILVITGRLDSTNSESFETLLTNLINAGTSQ
                     ILIDGTQMAYISSAGLRILLMIAKQLNRTRGRIVLCGLNESVKKVFDLTGLTGIFPLC
                     ATREEGLKSF"
     gene            2056..2751
                     /locus_tag="PKO06_05455"
     CDS             2056..2751
                     /locus_tag="PKO06_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69124.1"
                     /translation="MTRLGLWIALLLLIGGWSWAEGRDELVRLAESGNTDAQFKLGLD
                     LADNAKTPEEFEKAVQWWRKAAEAGHVRAQNNLGTSYAYGNGVDPDPAEAAKWWRKAA
                     EQGNDKAQANLASLYNLGSGVPQDVAEAAKWYRCSARLGNVEGQYNYGFFLFYGDGVQ
                     KDPVSALMWALLARTGGHPNGEKACATFMAGLTSDQISQAEAAAQGWKPATWEEVYSQ
                     EAQIASESSATPR"
     gene            2921..4231
                     /locus_tag="PKO06_05460"
     CDS             2921..4231
                     /locus_tag="PKO06_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69125.1"
                     /translation="MTRRVFICLLLCVCWASAPAAAQPFDLIIPDQTINDSLSRELRD
                     MQTIERDIQMEKLKSPASKLEAYIELGNLRLEQGKLEEARRFFEMAIELQPENMLANK
                     GLAMVHYHSGEFNETKAIFDRLSELYPLSDVLKSEAEKVRRHLRTSLDLGYMIREDSR
                     GVQEIHGIGELFFPSFTWPKLSARYRLEDYNYKDGNDRENSRLLSGTFNYVLDHRNQI
                     ALTYAPEIFSARKDVHGYDFQAVTGSGNLHASFNGGRHAFKENLATMREALGEDYATI
                     TFFGELNPRTRVSQTFTAADISDGNSRRRFDTDVLYFITRRGVPLFSLRARASQASYE
                     KQSDPFGNALSYWTPADFRGTELALGWERGVGSRWWWGCEAQANDNTWRDTSSTLTQQ
                     DTGLGFILHASYRYERGRIHARFTNTLHDFYRERSLGIFGSLDF"
     gene            complement(4235..4474)
                     /locus_tag="PKO06_05465"
     CDS             complement(4235..4474)
                     /locus_tag="PKO06_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69126.1"
                     /translation="MTPFEIGMLAGIVWAAVLVVWALADLTSGEPSPYLMLVSCIYEG
                     FDYSWRGICTGAVWAFADGFISGFCVSWIFQWLFG"
     gene            complement(4474..>4710)
                     /gene="amrB"
                     /locus_tag="PKO06_05470"
     CDS             complement(4474..>4710)
                     /gene="amrB"
                     /locus_tag="PKO06_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV69127.1"
                     /translation="GEALEVIQNLDDRRFFSMVRDEDRSICGVVPITVFLNCIRGWPQ
                     VTARKLAYSTSMDVMSHPAGVGYACVAFEQIRPG"
BASE COUNT         1075 a         1464 c         1292 g          879 t
ORIGIN      
        1 ggctcagatg cagcgcctcc tgattcacta cctgctgatc ggtgtctgta tctttctcgc
       61 caccgccgtc atcggcctgc aggcgatctc gagtgtctcg cggcatctgc agaccctgac
      121 ccagtcgatt caagatctcg tcaagtgcga gttcgccccg aaacccgaag cgcagaacga
      181 tctgcagcag atttccgaat cgtatcgcga tgaagtcggg tatctggccc gcgcatttct
      241 cagcatgcag agagccttgc agagctacct gcagaatctg gcccgcacca ccgcgcagaa
      301 agagaagatt cagagcgaac tgcgcatcgc ccacaacatc cagatgagca tggttccgca
      361 ccgctttcca cgcccagaag agaccggagg catcatgctg cacgcggttc tggaaccggc
      421 gcgcgaggtc ggcggcgatt tctacgactt cttcccgctc acgtcgagcg gcgaccgttc
      481 tgccgccttt gatcacgcat cgtcactccc ggtgcccgac cgactcgcgt ttgcgatcgg
      541 cgacgtttcg ggcaagggaa tgccggccgc tctgttcatg gcgatggtca aaacgctgtt
      601 ccgagccacc gcggcagcat gcagcgaccc ggcacgcatt ctcagcaaac tgaacgacga
      661 aatgtctcgg gacaatgcca cctccatgtt cacgacgatg caggtcggca ttatcgatct
      721 cagaacccgg atggtcacgc ttgccagcgg cgggcacaac tatccgctgc tgatttccgc
      781 cgagggaaaa gtccgagaga tgaaactgca aaacgctctc ccgatcggcg tctatgaatc
      841 cgtttcctat accaatcagg agttcaccct gcaggaaggc gatacgctgg tactgttcac
      901 cgacggcatc accgaagcca tgaacgagaa actggaagag ttcagtgatg agcgttttca
      961 ggcgattctc gaggggaaac agaacacggg gctcgaacag ctgtcgacga acgtgtatca
     1021 ggaagtccgt cgctatgccg gaacccagga tccttcggat gatgcgacac tgctgctcat
     1081 tcgggtcttc aagccgcagc agaaaatcgc caccggcggc ccgacacttt ccgagacagc
     1141 ggctttcggg ctggcgccca ctcttcccca acccgccgct ccgagcaact cgagtcaccg
     1201 gcagaccgtc gaaatccgtg attccgctga tctcaagcag attcaagatt ggctggatcg
     1261 cgaagcaccg gcctgggggc tcagccagcc ggacacacac gatttgcatc tcatcctcga
     1321 cgagctgatc gaaaacgtgc tgcggcatgg ctacgccaac acagagccgg cgcagattcg
     1381 cctggaggtg acggtcgccc ccgaatccat cgcgctctgt ctgcgcgaca ccggcagagc
     1441 gttcaatcct ctgctgcaac agagccccga caccacatcg gcggtcgcga cccgagcggt
     1501 cggtggctgg ggcattcatc tcgtgaaaca gtattccgat acgttgtcct atgaacgaca
     1561 aaacgatcag aacgtcgttc gtctctcgat cagacgacga gcgctgaaac cgcaggaagg
     1621 ggtaaccacc gacgcatgaa tctccagatc actcaggaac agcacggcca ggtcaacatc
     1681 cttgtcatca cgggaagact cgactcgacc aactccgagt cgttcgaaac cctgctcacc
     1741 aacctcatca acgccggcac ctctcagatt ctcattgacg gtacacagat ggcgtatatc
     1801 agcagcgcgg gcctgcgcat tctgttgatg atcgccaaac agctcaaccg cacccgcggc
     1861 cgcatcgtgc tctgcgggct caacgagagc gtcaagaaag tcttcgatct gaccggactc
     1921 accggtattt ttccgctctg cgccacgcgc gaagaaggtc tcaagagctt ctgacccgac
     1981 ctctcacgcc tcgaccggaa cccaggggga accgtttcgg cacacgcctg gcgcgctcat
     2041 gaagtaggga atttcgtgac cagactcggc ctgtggatcg cgttgctcct cctgatcggt
     2101 ggctggagtt gggcagaagg ccgcgatgag cttgtccggc tcgccgaatc cgggaacacc
     2161 gacgcccagt tcaagctcgg gctcgatctc gccgataacg ccaagacacc tgaagagttc
     2221 gaaaaggcgg ttcaatggtg gcgaaaagcg gcagaagcag gtcatgtcag agcgcagaac
     2281 aatctgggta ccagttacgc ctacgggaat ggcgtcgacc ccgatccggc cgaggccgcc
     2341 aagtggtggc gaaaagcagc tgagcagggg aacgacaaag cacaggccaa tctggcatca
     2401 ctgtacaatc tcggaagcgg agttccgcag gatgtcgccg aggcggcgaa atggtatcgc
     2461 tgctccgccc ggcttggcaa tgttgagggt cagtacaact acggattttt cctgttttac
     2521 ggtgacgggg ttcagaaaga cccggtgagc gctctcatgt gggcactgct tgcccgcacc
     2581 ggagggcacc ccaacggcga gaaagcctgc gccacgttca tggccggtct gacatcagac
     2641 cagatctcgc aggccgaggc cgctgcgcaa gggtggaaac ccgctacgtg ggaagaagtc
     2701 tattcccagg aagctcagat cgcctcggaa tcttctgcaa cacccagata aactcgtact
     2761 gcagacacag agccccacaa ccgccagacc cgacgactct gaccatcacg gctgtgttct
     2821 cgatctttcc cgaccgcttt ctgaaaccgc ctccagattt gtccggcatt cggccagagg
     2881 aatatcagct tctctcttca atctgccgat agtctgaagg atgactcgac gtgtcttcat
     2941 ctgcctgttg ctgtgtgtct gctgggccag cgccccagcg gcggctcagc catttgatct
     3001 gatcattccc gatcagacga tcaacgacag cctgagccgc gaacttcgcg acatgcagac
     3061 gatcgaacgc gacattcaga tggaaaaact gaagtcgccc gcctcgaagc tcgaagccta
     3121 tatcgagctt ggcaatctca ggctcgaaca gggcaaactg gaagaagccc gccgattttt
     3181 cgagatggcc atcgagctgc agcctgaaaa catgctggcg aacaagggcc tggccatggt
     3241 gcattatcac agtggcgaat tcaatgagac caaagccatc ttcgaccgtc tgagcgaact
     3301 gtatccgctt tccgacgtgc tgaaatcaga ggcagagaaa gtgcggcgac acctgcgcac
     3361 cagcctggat ctcgggtaca tgatccgcga ggactcccgg ggtgtgcagg agattcacgg
     3421 tatcggagag ctgttcttcc cctcgttcac ctggcccaaa ctctcggcgc gctatcgtct
     3481 cgaagactac aactacaaag acgggaacga tcgcgaaaac agccgacttc tgagcgggac
     3541 gttcaactat gtcctcgatc accgcaacca gatcgccttg acgtacgccc ccgaaatctt
     3601 cagcgcacgc aaagacgtgc atgggtatga ctttcaggcc gttaccggtt caggaaacct
     3661 gcatgccagc tttaacggcg ggcgtcatgc gttcaaggag aacctggcga ccatgcgcga
     3721 agcgctcggc gaagactatg ccacgatcac cttctttggt gaactcaacc ctcgaacccg
     3781 tgtctcacaa acgtttaccg ctgccgacat ctcggacgga aacagtcgtc gtcgattcga
     3841 caccgacgtg ctgtatttca tcacccggcg cggtgtgccg ctgttttcgc tgcgggcgcg
     3901 cgcttcacaa gcctcctacg aaaagcagag cgatccgttc gggaacgctc tttcctactg
     3961 gactccggcg gattttcgcg ggactgagct ggctctgggt tgggaacgcg gcgtcgggtc
     4021 acgctggtgg tggggatgcg aggcgcaggc caacgacaac acctggcgcg acacttcctc
     4081 gactctgacc cagcaggaca ccggcctggg cttcatcttg cacgccagct accggtacga
     4141 acgcggtcgc attcacgccc ggttcacaaa cacactgcac gatttctatc gtgaacgctc
     4201 actcggcatt ttcggcagcc tggacttttg agtttcagcc aaacagccac tgaaagatcc
     4261 atgaaacgca gaaaccgctg atgaagccgt cggcaaaagc ccagacagcg ccggtacaga
     4321 ttccacgcca ggaataatcg aacccttcat agatacagct gaccagcatc agatacgggc
     4381 tcggctcgcc gctggtcaga tcggccaggg cccagacaac cagaacggcg gcccagacaa
     4441 taccggccaa catgccgatc tcgaacgggg tcatcagccc gggcgaatct gctcgaaggc
     4501 aacacaggca tatccgacac cggccggatg cgacatgaca tccatgctgg tcgaatacgc
     4561 aagcttacgg gcggtcacct gaggccagcc ccgaatgcag ttcagaaaca ccgtgatcgg
     4621 aaccacgccg cagatgctgc gatcttcgtc acgcaccatc gagaaaaatc gacggtcatc
     4681 tagattctga atgacctcaa gtgcttcgcc
//
LOCUS       DAORHD010000193         4696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19236_length_4696_cov_2.818574, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000193 DAORHD010000000
VERSION     DAORHD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19236_length_4696_cov_2.818574"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="PKO06_05475"
     CDS             <1..1836
                     /locus_tag="PKO06_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV69128.1"
                     /translation="DERGQTLFARDHQSGFRTISVSGSLEILAVGDQGFAYLISPNGD
                     LVWKKRPAQALFGLISGSGRFLALVSREPTVVLADRNGAVKWVYRNLLATPGALDIAH
                     DGQTVVFACRDERGEGLAAVGFNGKPYDAFMGIDPLQDLSLHPSGDSLVAVDKGKGIY
                     CLNCIKSFGIWKGALNQTFHGVSFAGDVQTTLLYAKDGLITLLDGKGQPLWEHRFPHP
                     LLRACLSQDARQIFYASPEGRVGCLASRASRDLSRLEFVEIAPTALAGETSASPAPIA
                     WKKLWQFDLEPPSPGDKPRFTSWTGPDQVDYLLLWTGRNSLLCLNDDGEEVWKLSIPG
                     GIKDLAVDPDADLIIGVTGQAVLAYSLDGTQKFKFFGPFTRAGVFNSGAFILMTENGQ
                     IRVYSSSGQASHLVPLPAKANNLLVNDDLAFACSPTHVFYIDDRGTLRGQTPLEKPPV
                     VRQIMPFGTPRLSLLGLQNGDFFQLNQDGTEAAHGSTGVAPACLSCHAGEDVLFFAPE
                     VRTEIVIQQNRANKRFRVAVPQRVRSLHPHPRGCVAVTELDELMLITTQGQTALKQSI
                     PDRVLDLAAHASGDLLFILSEGSLAAYAPPDRAARPSSTTRFLEI"
     gene            1838..2302
                     /locus_tag="PKO06_05480"
     CDS             1838..2302
                     /locus_tag="PKO06_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV69129.1"
                     /translation="MPEMLHFTLIDVRHHHDLAQLVDLDRDAFAGDGLTTSNLALLTI
                     AGRVYALKCGDDILADAIVLPTCTPNLALLFSFAVLSSRRGQGLGRQLMGHLLPELAK
                     QGWKRLQLTVDPANVPAVHLYTRVFSFRQIDDLPDCLGPGKRRLLLEIALSI"
     gene            2442..4181
                     /locus_tag="PKO06_05485"
     CDS             2442..4181
                     /locus_tag="PKO06_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69130.1"
                     /translation="MRTLKQRRTGLALFFTLATIVVVSIIVYGMVFFMRGEVHLTENY
                     VDSTISLLLAEAGVEEAVYIIKTQGNNPKSPLYEMVTKKSEETVDLDLSSLEGKTSGI
                     PALVEGGKVKARVSWKHDPEATKELINQGLPADVARQGYLLIDSRGTYHNTSRQVQVK
                     KVLKATLVQSPFPGNTIGMIAPEHGLYFNKSHRDSFKINPTDFWDPWGFSVKGGKVFM
                     RDGAKIDLPKWLMLAKFRNELEHPWLDMGIGWNGYNGGTDLTGASEIEYANNPINRNY
                     YKWMGLFSWPWWVRTDNELYNSNTKKVDEPDPKKINLYPTEVYRRLANRLVDPDENPQ
                     HAKYFQDVNFQEAFGRNQVNYRNVIPLFGWGDWRKVANKYNRYLGNPTRAHDTSHAVE
                     INGVTFIKGDVYLEGWVKGKGLLVVEGDVYVGGDILTLPDDNGGKSAVGIIALRDPRD
                     TAVESPKSGRIIYKPHHDSDWSRMGVTHPFRNLSPRLEGCFHAQGGLELDTDSSLKKM
                     INMEVVGNLSMDYFDRRRMPNDISVKHFNWQDVLAQSDYDFTVDKEMKWSTKYDVAVM
                     KQIASWREVEPTL"
     gene            4432..>4696
                     /locus_tag="PKO06_05490"
     CDS             4432..>4696
                     /locus_tag="PKO06_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69131.1"
                     /translation="MVLLIFGQLFLVGAVLQEKLPLRSESPIPPAEVTMKAPEGKADI
                     APSARIASRTETPAAEPRRSPGLNPFRKPLPVTILGITRQDEKP"
BASE COUNT         1010 a         1436 c         1261 g          989 t
ORIGIN      
        1 gacgaacgtg gtcagacgct ctttgcccgc gaccaccaga gcggatttcg caccatcagc
       61 gtctcaggaa gcctcgagat tctcgccgtc ggagatcagg ggttcgcgta tctgatcagt
      121 ccgaacggtg acctggtctg gaagaagcgg ccggcacagg cgttattcgg attgatcagt
      181 ggatcagggc gctttctggc gctggtctcc cgcgaaccga ccgtggtact tgccgaccgt
      241 aacggtgcgg tcaaatgggt gtatcgcaac ctgctggcca caccgggtgc cctcgacatc
      301 gctcacgatg ggcaaaccgt cgtcttcgcc tgtcgcgatg agcgcggcga aggtctggcc
      361 gctgtcggct tcaacggcaa gccctacgat gccttcatgg ggatcgaccc cctgcaggat
      421 ctctcgctgc atccttccgg cgattcactg gtggctgttg acaagggcaa aggcatctac
      481 tgcctgaact gcatcaagag tttcggtatc tggaaaggcg cattgaatca aacttttcat
      541 ggagtctcgt tcgccggcga cgtgcagacg actctgctgt atgccaaaga cgggctcatt
      601 acactgctcg acggaaaggg ccaacccctg tgggaacacc ggtttcccca tccgctgctt
      661 cgagcctgcc tttctcagga cgcacggcag atcttctacg cctcaccgga agggcgtgtc
      721 ggctgcctcg cttcacgagc ctcacgcgat ctgagccggc tcgagtttgt cgagatcgcc
      781 cccaccgctc tcgctggaga aacgagtgcg tcaccggctc ccatcgcctg gaaaaagctc
      841 tggcagttcg acctcgagcc cccttccccc ggcgacaaac cccggttcac ctcgtggacc
      901 ggccctgacc aggtcgatta tctgctgctc tggaccggac ggaactcttt gctgtgcctc
      961 aacgacgacg gcgaagaagt ctggaaactc tcgattccag gaggcatcaa ggatctggcc
     1021 gtcgacccgg atgccgacct gatcatcggt gtcaccgggc aagccgttct ggcctactct
     1081 ctggatggaa cccagaagtt caaatttttt ggtccgttca ccagagcagg tgtgttcaac
     1141 tcgggggcct tcattctgat gaccgagaat ggccagattc gcgtctattc cagcagcggt
     1201 caggcttctc atcttgttcc cttgccggca aaagccaaca atctgctcgt caacgacgac
     1261 ctcgctttcg cctgcagtcc cacgcacgtt ttttacatcg atgatcgcgg caccttacgc
     1321 ggccagacgc cactcgaaaa gccaccggtc gtccggcaga tcatgccgtt cggcaccccg
     1381 cggttatcgc ttctggggct gcaaaacggc gattttttcc aactgaacca ggatggcacc
     1441 gaagcggcac acggatcgac cggcgtagct cctgcctgcc tgagctgcca cgccggcgaa
     1501 gacgtgttgt tcttcgctcc cgaagttcga accgagattg tcattcagca gaatcgtgcc
     1561 aataaacgtt tccgcgtggc ggtgcctcag agggtgcgtt cattgcaccc acacccgcgt
     1621 gggtgcgtcg cggtcacaga actcgatgaa ctgatgctga tcaccaccca ggggcaaacc
     1681 gctctcaaac agtcgattcc tgatcgtgtg ctcgatctcg ccgcacacgc ttccggcgat
     1741 ctcctcttta ttctttccga aggttccctg gccgcgtatg ctccgccgga ccgagccgca
     1801 cgacccagct ccaccacgcg gtttctggaa atctgacatg cccgagatgc tgcattttac
     1861 cctgatcgat gtgcgccacc atcatgatct ggcgcaactt gtcgaccttg atcgcgatgc
     1921 tttcgcaggc gacgggctga ccacctcgaa cctcgctcta ctgacgatag ccggtcgcgt
     1981 gtatgccttg aaatgcggtg atgacattct tgccgatgcc atcgtattgc cgacctgtac
     2041 gcccaatctg gcgctcctgt tcagctttgc cgtcctgagc agccgacgcg gtcagggatt
     2101 gggccgacag ttgatgggtc atcttctacc ggaactggcg aagcaaggct ggaaacgtct
     2161 tcagttaacc gtcgatccgg caaatgtgcc ggcagtccat ttgtataccc gggtattcag
     2221 ctttcgtcag atcgacgatc tgcccgactg cctcgggccg ggcaaacggc gcctgctgct
     2281 cgaaattgct ctttcgatct gattttcagc ccccatcccc gggcaacgag cccgtcattt
     2341 cgagtttctg aagaattctt tacaggttct gcatttgcgg gtacctcctg gttcttgcta
     2401 caattgagag tcaaaggaca ctcgaagcca agggagctta tatgcgtaca ctgaagcaac
     2461 ggcgcaccgg tctggcactc ttcttcacgc tggccaccat cgtcgtcgtc agcatcatcg
     2521 tctacggcat ggtcttcttc atgcgcggag aagtccacct gacggaaaac tacgtcgaca
     2581 gcacgatctc tctgctgctg gctgaagccg gagtggaaga agctgtctat atcatcaaaa
     2641 cccaggggaa caacccgaag agtccgctct acgagatggt caccaagaaa tcagaagaaa
     2701 ccgtcgacct cgacctgtcg tcactcgaag gcaagacttc cggtattccc gctctggttg
     2761 agggcggcaa ggtcaaagcc cgcgtctcct ggaagcatga tcccgaagcg accaaggaac
     2821 tgatcaatca gggcttgcct gccgacgtgg ctcgtcaggg gtatctgctg atcgactctc
     2881 gcggcactta tcacaatacc agccggcagg ttcaggtcaa gaaagttctc aaagcgacgc
     2941 ttgtacaatc tccgtttcct ggaaacacga tcgggatgat cgctcccgaa catggtctgt
     3001 atttcaacaa gagtcaccgc gactcgttca agatcaaccc caccgacttc tgggatccct
     3061 ggggtttcag tgtgaagggt ggcaaggtgt tcatgcgtga cggcgccaag atcgatctgc
     3121 ccaagtggct gatgctggcc aagttccgca acgaactcga acacccctgg ctcgacatgg
     3181 gcatcggctg gaacggctac aatggtggca ccgacctgac gggtgcctca gaaatcgaat
     3241 acgccaacaa cccgatcaac cgcaactact acaaatggat ggggctcttc agctggccgt
     3301 ggtgggttcg caccgacaac gagttgtaca attcgaacac caagaaagtc gacgagccgg
     3361 atccgaagaa gatcaacctc tatccgaccg aggtgtatcg ccgcctcgcc aatcgcctgg
     3421 ttgatcctga tgagaatcca cagcatgcca agtatttcca ggatgtgaac tttcaggaag
     3481 cgttcggtcg caatcaggtg aactaccgca atgtcattcc cttgttcggg tggggcgact
     3541 ggcgcaaggt ggcaaacaag tacaaccggt atctgggaaa cccgactcgc gcccacgaca
     3601 ccagtcatgc agttgaaatc aacggcgtca ccttcatcaa gggcgatgtc tatctcgaag
     3661 gctgggtgaa aggcaaaggt cttctggtgg ttgaaggcga cgtctacgtg ggcggcgaca
     3721 ttctcaccct gcccgacgac aacggcggca aatcggcggt cggtatcatc gctctgcgcg
     3781 accctcgcga cacagccgtt gaaagcccga agtcgggacg catcatctac aaaccccatc
     3841 acgacagcga ctggtcacgc atgggcgtca cgcacccgtt ccgcaatctg agcccgcgcc
     3901 tcgaaggctg tttccacgct cagggtggtc ttgaactcga taccgactct tcgctgaaga
     3961 agatgatcaa catggaagtc gtcggcaacc tctcgatgga ttacttcgat cggcggcgca
     4021 tgccgaacga tatctcggtc aagcacttca actggcagga cgtactcgct cagtctgatt
     4081 atgacttcac ggtcgacaaa gagatgaagt ggagcaccaa gtacgacgtg gccgtcatga
     4141 agcagattgc cagctggcgc gaagtcgaac caaccctcta aagtacaagc gacaatccgc
     4201 gcaggtgctt ttcctgcgcg gattttcatt ttagacacag gtctcccttc ccggccgcca
     4261 tccccagccc ccccccgaac ctcttccgct tcgccttttc gcggcagtga acgcaaaagc
     4321 ccgtgactct gcccgtggcg taataatgtt ctccgactga tctgccgata ggaagattga
     4381 tcgaattgtc agaacgggga aaaaggtatc aaagacacgc tgaaacaatt ggtggttctg
     4441 ctcatcttcg gccagctgtt tctggtcgga gccgtcctgc aggagaaact tcccctgcgg
     4501 tcagagtccc ccattccccc tgcagaagtg accatgaaag ctcccgaagg gaaagcagac
     4561 atcgcaccat ctgcgcgaat cgccagtcgc acggaaacac ccgcggcgga acccaggcga
     4621 tctccagggc tcaatccgtt tcgcaaaccg ctgcccgtga caatactcgg aatcacacgc
     4681 caggatgaga agccct
//
LOCUS       DAORHD010000194         4691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19263_length_4691_cov_5.493313, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000194 DAORHD010000000
VERSION     DAORHD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19263_length_4691_cov_5.493313"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1227
                     /locus_tag="PKO06_05495"
     CDS             85..1227
                     /locus_tag="PKO06_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNV69132.1"
                     /translation="MQTHLNTTNVTKTQLVGLITILEEVPDPRVTATVDHDLPDILTI
                     ALCTILCGGDSFYDMEEFGQVRLDWLKTFLRLRNGAPTHDTYNRVFQAIDPDPFSDCL
                     SRWTQSVRAVLGGEVVALDGKAMRRALKKGEDPRVIVSAWATQSGLLLGQRKVKDKSN
                     EITAVPELLRALELAGCIVTADALHCQKNTAKEIKEADAEYVLALKGNQGTAYTEVKT
                     FLDEAIARKETHLVTTETTDKEHGRIEVRRYWQTEKLEWFADRKEWEGLKSVGVVEAR
                     RTVNGKESVERRYYLSSLNNDAERFAKAVRGHWGVENSLHWVLDVVFGEDDSRARSGY
                     AAENLAATRRLAINLLRRDKTCKRSIKGKLMRAAIDPDYLKLILKS"
     gene            complement(1200..3878)
                     /locus_tag="PKO06_05500"
     CDS             complement(1200..3878)
                     /locus_tag="PKO06_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69133.1"
                     /translation="MQSKRTEKIDSRIEREMEDLVTDLRSTLVTKRLKAVKSLGRLKT
                     PLAVDPLREVLLDRSREVRCAVIEALGMINPPGVESILIPQAADRSADVRLRVAIALG
                     KCTTEEAINGLFDLLRDPKDDVAIMAAKALARHPSASLARLMRFFGEKSWKMRSRAAM
                     AIARMGRKPIDALLAGLEDTDPNVRFWSALCLGRLKDKTHTRELLAKLSDPTTGVRIA
                     ALRALREVGDPSIVSKLFEALSQPSEQVRDLIYDILKDFGNHSIPFLMESLSSEFWMG
                     RSLAARALSEMGSDAIHPLTQALESQDKERRFWAIKILSRMRERAALPEIRKFLTDSD
                     SEIRMAAVQGMGDLCDPESIPLLIERFLDPSWVVRREAHRSVVRFGDRAVANLIKALE
                     SVDEDVRYWSLRSIAEIKPPGLFPILVKLLKDRSWNVRKTTAEALSAYGEDALLELTN
                     LATEGDSEVRYWVLQALGKIGSQISLPLLFRALEDPSEAIRISAQKALANFGSSIIDD
                     LIALLKNDKRRLLESVAGTLQLMPAQLVLPRLCQALGKFDEHISYWVRRALAGFGPLA
                     RKAVEPLLESKANEIRRQAIFAFAQVGLPEDAEIIIRHLKDEFWPARIAAAEVLGKLR
                     NPLAVEPLMEALEDDDEDLAIAAVRSLGVLEDDRAVPALLSTLWRESWTLKYTVIDIL
                     GRMQVRRAVPELLRMLDEDTLDLRIPLIRALGNIAHPDSFKPLQERFLRETESECRLA
                     FIEAFGSLQKPEIIPTLMGLLKEERPWDEKRLLIRTLGLLRAMEAREQLVTFLRDPDL
                     FISREALLALKNILPPEEGAALENRLVAARRQQEKFQQHFREGMRQMRLGAMVEAERD
                     LKNALKLNPRAVRPRISRPPPARRADAYQDLRMSLR"
     gene            4005..>4691
                     /locus_tag="PKO06_05505"
     CDS             4005..>4691
                     /locus_tag="PKO06_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNV69134.1"
                     /translation="MPQTSGETPSATTAAVSNFIRDIIADDLKTGKHQSIVTRFPPEP
                     NGYLHIGHAKSICLNFGLARDFGGVCHLRMDDTNPIKEDQEYVDSIIDSVRWLGFDWQ
                     GKLFYASDYYDQLYAWAIELIKKGLAFVCELTADQIREHRGTLTKPGRESPFRNRTVE
                     ENLDLFERMRKGEFPEGTRTLRAKIDMNSPNINMRDPVLYRIMKTTHHRSGDKWQIYP
                     MYDFAHPLSDA"
BASE COUNT         1032 a         1318 c         1427 g          914 t
ORIGIN      
        1 gcagggctga cgcataccaa ggattccgtc attttctgtg atttttgtat gtactttgta
       61 tttacaatgc gtacatgaga agggatgcaa acgcatctca acacaacgaa cgtcacgaaa
      121 acacaactcg tcggcctgat cactatcttg gaggaagtgc cagatccgcg cgtcaccgcc
      181 acggtggatc acgatctgcc agacattctg accatcgcgc tctgcacgat tctctgcggc
      241 ggcgacagtt tctacgacat ggaagagttc ggccaagtgc ggctggactg gttgaaaacc
      301 ttcctgcgcc tgcgcaacgg cgcgcccaca cacgacacct acaaccgcgt ctttcaagcg
      361 attgatcccg acccgttcag cgactgcctc tcccgctgga cgcaaagcgt gcgcgccgtg
      421 ctgggcggcg aagtggtggc actggacggc aaggccatgc gccgcgcgtt gaagaaaggc
      481 gaagacccgc gcgtgatcgt cagcgcgtgg gccacgcaga gcggattgct gctgggccag
      541 cgcaaagtca aagacaagag caacgagatc accgccgtgc cggagttgtt gcgcgccttg
      601 gaactggccg gctgcatcgt caccgccgac gcgctgcact gccagaagaa caccgccaaa
      661 gagatcaagg aagccgacgc agagtatgtg ctggccctca aaggcaacca aggcacggcc
      721 tacacggaag tgaaaacctt tctggacgaa gccatcgcgc ggaaagaaac gcacttagtc
      781 accacagaga ccacggacaa agaacacgga cgaatcgaag tgcggcggta ttggcagacc
      841 gagaagctgg agtggtttgc cgatcggaaa gaatgggagg gattgaaaag cgtgggtgtg
      901 gtggaagccc ggcggacggt caacgggaag gaaagtgtgg aacggcgata ttatctgagc
      961 agcctgaaca atgatgccga aagatttgcc aaagccgtgc gggggcattg gggcgtggag
     1021 aacagcctgc actgggtctt ggatgtggtg ttcggcgagg acgacagccg cgcccgcagc
     1081 ggctacgccg ccgagaacct ggccgccacc cgccgactgg ccatcaatct cctgcgacga
     1141 gacaaaacct gtaaacgcag catcaaaggc aaactcatgc gcgccgccat tgacccagat
     1201 tacctcaaac tcatcctcaa atcctgatat gcgtcagccc tgcgagctgg aggcggccga
     1261 gaaatccttg gccgaactgc gcgtggattg agcttgagcg cattcttgag atcgcgttcg
     1321 gcttcgacca tcgcgccgag acgcatctgg cgcatgccct cccggaagtg ctgctgaaac
     1381 ttctcctgct ggcggcgggc ggccaccagt cgattctcga gggcggctcc ttcttcggga
     1441 ggaaggatgt tcttgagggc gagcagggct tcacggctga tgaagagatc ggggtcgcgc
     1501 aaaaaggtca ccagctgttc cctggcttcc atggctcgaa gcagaccgag ggttctgatg
     1561 agtaagcgct tttcgtccca gggacgctct tccttgagaa gccccataag cgttgggatg
     1621 atctcgggtt tttgcagaga gccgaaagct tcgatgaacg ccaggcgaca ctcggactcg
     1681 gtttcgcgca agaaacgttc ctgcagaggc ttgaagctgt cggggtgcgc gatgttgccg
     1741 agcgcgcgaa tcagaggaat acgcagatcg agcgtgtctt cgtcgagcat gcgcagcagt
     1801 tcgggaacgg ctcggcgaac ctgcatgcgg ccgagaatgt cgataaccgt gtacttgagc
     1861 gtccaggatt cgcgccacag ggtggaaagc agagccggca ctgcccggtc atcttcaagc
     1921 acgccaagag agcgcaccgc ggcgatggcc agatcttcgt catcatcttc gagtgcttcc
     1981 atcaatggct cgaccgccag cgggttgcgc agcttgccca ggacttcggc ggcggcgatg
     2041 cgggcaggcc agaattcgtc cttcagatgg cgtatgatga tttcggcgtc ttccgggagt
     2101 ccgacctgag cgaaagcgaa aatggcctgg cgacggattt cattggcttt gctttcgagc
     2161 agaggttcga ccgctttccg ggccagagga ccgaacccag ccagcgctcg acgcacccag
     2221 tagctgatat gctcgtcgaa tttgccgagc gcctggcaca aacggggcag gaccagctgt
     2281 gccggcatga gctgaagagt accagcgacg ctttccagaa gacggcgttt gtcatttttg
     2341 agcagggcga tcaaatcatc gatgatcgaa gagccgaagt tggccagcgc tttctgcgcc
     2401 gagatgcgaa tggcctcgga tggatcttcg agcgcccgga acagcagagg cagcgaaatc
     2461 tgtgaaccga tcttgccgag cgcctgcagc acccagtatc gcacctctga atcgccttcc
     2521 gtggcgagat tcgtcagttc gagcagagcg tcttcgccgt acgcgctgag cgcttcggcg
     2581 gtggttttgc gtacgttcca ggagcgatct ttcaacaact tcaccagaat cggaaacaga
     2641 cctggtggtt tgatctcggc gatcgatcga agcgaccagt agcgcacgtc ttcgtcaacg
     2701 gactcgagtg ctttgatcag attggcgacg gcgcgatcgc cgaaacgtac gaccgaccga
     2761 tgcgcctcgc gtcgtaccac ccaggaaggg tcgagaaacc gctcaatcaa cagcggaatg
     2821 gactcgggat cacacaggtc gcccattccc tgcacggcgg ccatgcggat ctcgctgtcg
     2881 gaatcggtga gaaacttgcg gatttcaggg agagccgcgc gttctctcat gcgactgaga
     2941 atcttgatgg cccagaaccg gcgctctttg tcctgacttt cgagggcctg ggtcaggggg
     3001 tgaatggcat cgctgcccat ttcggaaagc gctcgtgccg cgagagagcg tcccatccag
     3061 aattcgctcg agagcgattc catcaggaag gggatcgaat gattgccgaa atccttcaga
     3121 atgtcgtaaa tgagatcgcg aacctgctca gatggctgag aaagcgcttc gaacaatttg
     3181 ctgacgatac tgggatcacc gacctcgcgc agggcgcgca gtgcagcgat acgaacgccg
     3241 gtggtggggt cagacagttt ggcaagcagc tcgcgcgtgt gcgttttatc cttgagacgg
     3301 ccgaggcaga gagcagacca gaaacgcaca tttgggtcag tgtcttcgag gccggccagc
     3361 agggcatcga tgggtttgcg tcccatgcgg gcaatcgcca tagcagcgcg tgatcgcatt
     3421 ttccaggact tttctccgaa aaaacgcatc agtcgagcga gcgaagctga cgggtggcgg
     3481 gccagtgcct tcgcggccat gatggcgaca tcatctttcg ggtcacgcaa cagatcgaac
     3541 aggccgttga tcgcttcttc ggtggtgcac ttgccgagag cgatggcgac gcgtaaacga
     3601 acgtcggccg agcggtcggc tgcctgtgga atcaggatcg attcgactcc gggcggattg
     3661 atcatgccga gcgcttcgat aaccgcacat cgcacttcac gagagcgatc gagcagcact
     3721 tctcggaggg ggtcgacggc cagaggggtt ttcagacgcc cgagtgactt gacggctttc
     3781 aggcgcttgg tgaccagggt tgaccgcagg tcggtcacca ggtcttccat ttcgcgttca
     3841 atgcgactgt cgatcttttc ggtgcgcttg ctctgcatgt ctcactctcc cggggtcaaa
     3901 tggcacgaga ttataccata cccgtggtct cggaagcgca tttgtgctat attccggcca
     3961 attctgagca agggggccgt tctcctatga cgaacaccca gaacatgccg cagacaagcg
     4021 gcgagactcc ttcggcgacc accgccgcag tttcgaactt cattcgtgac atcatcgccg
     4081 acgatctgaa aaccggcaag catcagagca tcgtgacgcg ctttccgccg gaacccaacg
     4141 ggtatctgca tatcggtcac gcgaagtcga tctgcctgaa cttcggtctg gctcgcgatt
     4201 ttggtggcgt ctgccacctg cgcatggatg acaccaaccc gatcaaggaa gatcaggagt
     4261 acgtcgattc gatcatcgac agcgtgcgct ggcttggctt tgactggcag ggcaagttgt
     4321 tttacgcttc agattattac gatcagctct atgcgtgggc gatcgagttg atcaagaagg
     4381 ggctggcgtt tgtctgtgaa ctgaccgccg atcagattcg cgagcatcgc ggcacgctca
     4441 ccaagcccgg gcgagaaagc cctttccgca atcgcacggt ggaagaaaac cttgatctgt
     4501 tcgagcgcat gcgtaaaggc gagtttcctg aaggaactcg taccctgcgc gcgaagatcg
     4561 acatgaactc gccgaacatc aacatgcgtg atccggtgct gtatcgcatc atgaaaacga
     4621 cacaccatcg cagcggggac aaatggcaga tctacccgat gtatgatttc gcgcaccccc
     4681 tttcggacgc g
//
LOCUS       DAORHD010000195         4689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19279_length_4689_cov_2.898576, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000195 DAORHD010000000
VERSION     DAORHD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19279_length_4689_cov_2.898576"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="PKO06_05510"
     CDS             complement(<1..2351)
                     /locus_tag="PKO06_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV69135.1"
                     /translation="MSHESGLSARIIGQIALMQSVVAQFPDRLTMIRFVCHGLTDVTG
                     VARVEYAIVDDRGAPSESPVATQTNETIRSFPVRHHENMHAMLYFVLNNPDAFAPYVP
                     YVENFCQMLAVIMEEHRQREMNRELLEKLEKRVAERTAELEREIDVRRQAEVALRASR
                     EEYRNLVEHLPQRIFVKDGSSVYLSCNAAYADDLGIAPEDIVGKDDFAFYPSELATQY
                     RADDRKVMDSGVRAEFEERYLVRNRWRWIRTIKVPFRGADGKTEGVLGIFDDITEKRQ
                     AEEALRESEQRFRAMFDNSNDGIVIAEVATRRLRYANPAICRMLDYSEADLLSRQADD
                     LHVDAARPQAMDLFRRMASGETTFGVDVPLRRRDGTEIVCEVGAHVIEIQNLPCLMGV
                     FRDVTRQRQLEEQLRQAEKMQAIGQLAGGVAHDFNNMLAVILGHSEIALEQFPADHDL
                     HKNLAEIHRAAQRSAELTSQLLAYARKQTIMPRVLDLNQTIDGLMSMLRRLIGENITI
                     SCLLKAELWPVCLDPSQVSQILTNLAVNARDAISGTGSIVISTENVVCDAAFCREHGW
                     QNPGDYVLVKVTDTGCGMGEETLSHLFEPFFTTKDVGRGTGLGLATVYGIVRQNNGNI
                     LVTSRPGKGSCFLIYLPRHDGPQAHVEPVSPHPETGGRETILLVEDEPEVLELTSLLL
                     QRLGYCVLPESNPKRAVQLAAAHSGRIDLLMTDVVMPEMNGRELAKAIQAKFPELKCL
                     YMSGYTADVIARQGVLEQDMHFIQKPFNLSQLATRIREALNGKR"
     gene            complement(2353..2967)
                     /locus_tag="PKO06_05515"
     CDS             complement(2353..2967)
                     /locus_tag="PKO06_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNV69136.1"
                     /translation="MCAEHCVSLGFTDEKVPAGTHICLIFTDERERVDALLKFLLSGL
                     QSGERMACFSERIEEKTVREYLAKHGISYDECRERDAITLAGTSSVYFKDGVFDPDRM
                     LKTLSQFYRDSLRQGFTASRVIGEMTPDVERVPGGERLMEYECRVTKLVRETPVTSVC
                     QYDATQFDGATIMNVLKVHPKMIVNGAVVQNPFFIEPEEYLKGL"
     gene            complement(3011..3799)
                     /locus_tag="PKO06_05520"
     CDS             complement(3011..3799)
                     /locus_tag="PKO06_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69137.1"
                     /translation="MNLRLENGRWWLASCLLVIVLGLSGFTLEEIRELKNMGFTNEQI
                     VELQRAGGTMPTAMASMTVPVVPTPPALSVGGERATYLTDLQKRHKGLLIVCATKVWP
                     FRGPGYLNVDIKFPNGKWGGIGRANLLEYLMNGQVTPPIFEKLETDDSCDGEIVVTRQ
                     LPIIVSRYYGELEVDAGTYEMKLTRNFRMTEKESGIGKTKRHKKFHGVQIKPGMVTIL
                     SYSWDENNKFGLDHVMPIEHRSFVDTVTGRFGAAVVEVKVQHNE"
     gene            complement(3796..4557)
                     /locus_tag="PKO06_05525"
     CDS             complement(3796..4557)
                     /locus_tag="PKO06_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69138.1"
                     /translation="MTDFTPVAGLLGGALIGASAALLLLMEGRVAGVSGIFARALGSP
                     LRNLRWRLPFLAGLVLVLCLGGYFSWVAGIIQREMNPKDLAKVSGAYLSDFIVSTLKG
                     YSDNLIKMAPDYAGQAIDTGFLQTQSWLKDVRFLVIGLIDERISMTQSLLMTLLDRSY
                     EEHVRELKPLISNIKTEAGRKAFADYFVTLLETPFAVESVRIDVESLHLTLEALHEKL
                     VKLSKAENLNQSEEAERALLVAAREFWNRYQGAEK"
BASE COUNT          989 a         1427 c         1246 g         1027 t
ORIGIN      
        1 cgcttcccat tcaacgcttc gcggatgcgg gtcgccagtt gcgagagatt aaacggcttc
       61 tggatgaaat gcatgtcttg ctctaatacg ccctggcggg caatgacgtc ggccgtgtat
      121 ccggacatgt acaagcactt gagctcggga aacttggcct gaatcgcttt tgccagttcg
      181 cgaccattca tttcaggcat gacgacgtcg gtcatcagga gatcgatcct gccgctatgt
      241 gcggctgcga gctgaaccgc ccgtttcggg ttcgactctg gcagcacgca ataacccagc
      301 cgctgcagaa gaagagacgt cagctccaag acttccggct cgtcttccac cagcaaaatc
      361 gtctcccgtc ctccggtttc ggggtgtggc gatacgggct caacatgtgc ttgcggtccg
      421 tcatggcgcg gcaggtagat gagaaagcaa cttcccttac ccggtcgact ggtcaccaga
      481 atgttgccgt tgttctggcg aacaatccca tacacggtgg ctagacctag tccggtaccg
      541 cgaccgacat ccttggtcgt aaaaaagggt tcgaagagat gcgacaacgt ttcttcgccc
      601 atgccacacc ccgtgtcggt gacctttacc agcacataat ccccggggtt ctgccagccg
      661 tgctcgcggc aaaaagcggc gtcacacacg acattttcgg tcgagatcac aatgcttccc
      721 gtgccggaaa tcgcatcgcg cgcgttcacg gcaagattgg tcaaaatctg actgacctgt
      781 gaaggatcca ggcacaccgg ccacaattcc gctttcagca ggcaactgat ggtgatgttc
      841 tcaccgatca accgtcgcag catgctcatc aggccatcga tcgtctgatt cagatccagc
      901 actcgcggca tgatggtctg ctttcgggcg taggccagca attgactggt gagttcagca
      961 gaacgctgag ccgcacggtg aatctcggca agattcttgt gcaggtcgtg atcagcaggg
     1021 aactgttcaa gcgcgatttc cgagtgcccg aggatgaccg ccagcatgtt gttgaaatca
     1081 tgcgccaccc cacccgctaa ttgaccgatc gcctgcatct tttcggcctg ccgcagctgt
     1141 tcttcgagtt gccgctgccg ggtcacgtca cgaaaaactc ccatcaggca tggcagattc
     1201 tgaatctcga tcacatgcgc cccaacctca cagacaatct cggtgccatc tcgacgcctc
     1261 aacggcacat cgacaccgaa ggtggtttcc ccgctcgcca tacgtcgaaa gagatccatc
     1321 gcctgtggtc gggcggcgtc gacatgcaga tcgtcagcct gtcggctcag caagtctgcc
     1381 tcggaataat caagcatgcg acaaatcgcc ggattcgcat accggagacg gcgtgtcgcg
     1441 acctccgcga taacgatgcc gtcgttggaa ttgtcgaaca tggcgcgaaa tcgctgttca
     1501 ctttcccgca gagcctcttc ggcctgacgt ttctcggtga tgtcgtcaaa gatcccgagc
     1561 accccttctg tctttccgtc tgccccgcga aatggcacct tgatcgttct gatccaacgc
     1621 catcggtttc gtaccagata acgttcttcg aactccgctc gaactccaga atccatgacc
     1681 tttcgatcat cagcgcgata ctgagtggcg agttctgatg ggtagaaggc gaagtcatcc
     1741 ttgcccacga tgtcttccgg ggcaatacca agatcgtcgg cataggcggc attgcaggaa
     1801 aggtacacgg acgatccgtc cttgacaaag atgcgctggg gcagatgttc aacaagattt
     1861 cgatattcct cgcgactcgc ccgcaacgcg acttcggctt gacgacgcac atcgatttct
     1921 cgttccagtt cggccgtacg ttccgcaacc ctcttttcca gtttttccag taactctcga
     1981 ttcatctcgc gctgccggtg ctcttccatg atcaccgcga gcatctggca gaagttttcg
     2041 acatagggaa catacggtgc aaatgcgtcc gggttgttca agacgaaata cagcatcgcg
     2101 tgcatgtttt cgtggtgtct gaccgggaac gagcggatcg tctcgtttgt ctgcgtcgcc
     2161 acaggagatt ctgacggcgc accccgatca tccacgatgg catactcgac gcgggcaaca
     2221 ccggtgacat ccgtgagacc gtgacagaca aaccggatca tggtcagtcg atccggaaac
     2281 tgggcgacca cgctctgcat caaggcaatc tgcccgatga ttctggccga caacccgctc
     2341 tcgtgactca cgttacaagc ccttcaggta ttcctcgggc tcgatgaaga acgggttctg
     2401 cacgacggca ccgttcacga tcatcttcgg gtggaccttg agcacattca tgatcgtcgc
     2461 cccgtcaaac tgggtcgcat catactggca gacactggtg acgggagttt cgcgcaccaa
     2521 cttggtcacc cggcactcgt attccatcag gcgctcaccc cctggcaccc gctccacatc
     2581 aggtgtcatc tcaccgatga cccgcgaggc cgtgaacccc tgccgaagcg aatcacggta
     2641 gaactgagag agcgttttca gcatgcgatc agggtcgaat acgccatctt tgaagtagac
     2701 cgaactggtt ccggcaagag tgatcgcgtc acgctccctg cattcatcgt aggaaatgcc
     2761 gtgttttgcc agatactccc gcaccgtctt ctcctcgatc cgttcggaaa agcacgccat
     2821 gcgttccccg gactgcaagc cgctcaacag gaacttgagc agcgcgtcaa cccgttcccg
     2881 ctcatcggta aagatcagac agatatgagt gccggccggc accttttcat ccgtgaaccc
     2941 gagagagaca cagtgttctg cacacatggg gtttgctcct tgtataaaaa atcaatacga
     3001 gcgctcgccc tcactcgttg tgttgcactt tcacctcgac aacagcggca ccgaatcgcc
     3061 ccgtcacggt gtcgacaaag ctgcggtgct cgatcggcat gacgtggtca aggccgaatt
     3121 tgttgttttc gtcccaggag taagacagga tcgtcaccat tcccggcttg atctgcaccc
     3181 catgaaactt cttatgccgt ttggtcttgc cgatgcccga ctctttctcg gtcatgcgga
     3241 agttccgggt cagcttcatt tcatacgttc cggcatccac ttccagttcg ccgtagtaac
     3301 gtgagacgat gatcggcagc tgccgcgtga ccacgatttc tccgtcgcag gaatcatccg
     3361 tttcgagctt ctcaaaaatc ggcggcgtca cctgtccgtt catcagatac tccagcagat
     3421 tggcgcgacc gattccgccc cacttgccgt tcggaaactt gatatcgacg ttgagatacc
     3481 ccgggccgcg aaacggccag accttggtgg cacagacgat gagcaaacct ttatgccgct
     3541 tctgcaggtc tgtcagatac gttgctcgtt ctccgcccac ggaaagcgcc ggaggcgtcg
     3601 gcaccactgg gactgtcatt gaagccatcg ctgtcggcat ggttccgccc gctctctgca
     3661 actcgacgat ctgttcgttc gtgaagccca tattcttcag ctcgcgaatc tcttcgagcg
     3721 tgaaaccgga aagaccaagg acgatcacca gcagacagga ggcaagccac caccgcccgt
     3781 tctcgagtcg aaggttcact tttccgctcc ttgatagcga ttccagaact cgcgtgcggc
     3841 cacgagcagg gctcgttccg cttcctcgct ctggttcagg ttttctgcct tgctcaactt
     3901 gaccagtttc tcatgcagag cttcgagcgt gagatgaaga ctctcgacat cgatgcgcac
     3961 gctctcgacc gcgaatggcg tttcgagcaa cgtcacgaag tagtcggcaa aggccttgcg
     4021 accggcttcg gtcttgatat tgctgatcaa gggcttcagt tcgcgcacat gttcttcata
     4081 ggatcgatcg agcaatgtca tgagcagact ctgagtcatc gagatccgct catcgatcag
     4141 accgatcacc agaaatcgca cgtctttcag ccagctctgc gtctgcagaa aaccggtatc
     4201 tattgcctga ccggcgtaat caggcgccat cttgatcaga ttgtccgagt agcctttgag
     4261 tgtcgaaacg atgaaatcag agagataggc cccactgacc ttcgccagat ctttcgggtt
     4321 catctcacgc tgaatgatgc cggcaaccca cgagaaatag ccacccaggc ataacacgag
     4381 cacgaggccc gcgaggaacg gcaggcgcca ccgcaggttg cgcaggggcg atccgagcgc
     4441 ccgggcgaag atgccgctga cgcccgcgac ccgcccctcc atgagcagca agagcgccgc
     4501 gctcgcgccg atgagcgcgc cgccgagcag gccggcgacc ggggtgaagt cggtcacgtc
     4561 cagcctcgta ctgtcagcat attgaacaca tgtccgttat acgggaattt tggacgcggg
     4621 caagcggggc gtatctgcgc gcgtgaggtc cggggatgtg tcctgtcgcg gccggtacct
     4681 ggggcgcat
//
LOCUS       DAORHD010000196         4688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19285_length_4688_cov_2.953162, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000196 DAORHD010000000
VERSION     DAORHD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19285_length_4688_cov_2.953162"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKO06_05530"
     CDS             complement(<1..485)
                     /locus_tag="PKO06_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69139.1"
                     /translation="MDNTVYKIALAGFLHDIGKFAQRAEGAPTSGGLESAVGFFPNPE
                     FINRHRQQYQPEYNGHFTHTHAVYTAAFIDVRKSAKTILDKRLKSDSEFAARFYKALA
                     LFLADRMRNTIRHLGYGKLSDEPARDEDALDLDELDIEVLDNVHLAGARFERILKKLM
                     A"
     gene            complement(488..1615)
                     /gene="cas6"
                     /locus_tag="PKO06_05535"
     CDS             complement(488..1615)
                     /gene="cas6"
                     /locus_tag="PKO06_05535"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HNV69140.1"
                     /translation="MNTIAMQSMINESSVRPAQPCSNSKHACAVSLPYCVLRASFRTE
                     SEIRFPQNPVNTLRGGLGFHLKRAVCFHRARTAEGELVACAGCLLEKNCVYALLMETA
                     APGTEAELATGGARETPRPMHFAAEFAGPICLAPGDTFMFIITLFGRASEAIPYLIFA
                     LKELGQHGFTREQIRAPLDEVRMLGELSPMVYKRGVERLEPIVPDVLSLVPPLVSEAD
                     ETRDVELRFLTPVSFKDKQTGKVSNLPDFSRLIGSILRRLTSLCVVSGIPAPQWDFTG
                     IMEQARTVQTVDVNGRFVRWERYSQRQHQRSYWGGMVGNVVYRGRLAPFLPLLRAGEI
                     FHVGRGISFGQGRYRIQRIAVPEGDACSTEHPDSEPIFLWE"
     gene            complement(1842..3842)
                     /locus_tag="PKO06_05540"
     CDS             complement(1842..3842)
                     /locus_tag="PKO06_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69141.1"
                     /translation="MLVPLLALQRRGCAFERIDLLATPFTKNVARQIADYSTTWIEVC
                     TITEIESDNRTRDERGPKEMEMIRRIMSSEGTLVFNLNGGMNFQIACGLLQAPLERTR
                     FLYSDSDRMTLFRIDSKRKVHTEDLPLCSEHLDVLRLQRVAFSPLEGEAATGQNERVS
                     VGVKIGKVRFDAVVNIENTLRFYVFLERPFLEPGDTVKEALLRKTRDIMTLATGRSRF
                     AQLFHREVIVVTARKNVQERLETEGRGKIRVERWPLERIRATYRNFSGWVRSLARPQP
                     VHSVRSASTRNVEQRGKRNQQGTSLAVFLGSDILPTLIAIRSHAPSNLYLFYDAGNTQ
                     IVQTAELWMKEWVKLGVSYVAFISTGFLDLQLLEAEIRAERLEVNSTPGTKLQGALLI
                     LLGRMSNPEVVSVWTINNATRRLSDLTGHLPDRPLKGPSPLKLLTLRGENIHQAGEDE
                     GVLARRKWIMDALTNWFRRVSESGNPLQRFSAGQNAEYGEMTCHVTGNDAFLRSKTSK
                     AEVRIDLRQDQWFERWVGWRFFVAGKADEVAVRLRTQWRHESDSVACRGDELSDIDKP
                     YVSFKSDLDVVLRFGHRYMVVSCKSGKIESTNAHADETAATAAMFGRFTVPVLVQLPF
                     TGNPERLNDTWRLGGKHLLDDAELYKLIEHMLASHSTTRPPQ"
     gene            complement(3995..>4688)
                     /locus_tag="PKO06_05545"
     CDS             complement(3995..>4688)
                     /locus_tag="PKO06_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69142.1"
                     /translation="PQLLMYVLQPSPRIVSFLESVRQQACENGLELLTFDFHGEEGRR
                     RFLELLTDPARREAFMKIYDLYVPTAGYAVEMPEPWPVNLDFTARHLRVGTSVTTLEP
                     LLLAIYAGFLSNKRSCSETSCRAEHGDCAFRCFLSIADMCRNGFPHRVAEFYRRSYGW
                     GGQREEDMLARLENGLETEAMRQYISKINRRLRASLCDHPMLDRLLIVPIGRYGNTRY
                     GVRLPRHAVPKE"
BASE COUNT         1053 a         1337 c         1270 g         1028 t
ORIGIN      
        1 taggccatca gtttcttgag gatgcgctcg aaccgggctc ccgccagatg cacattgtcc
       61 agcacttcga tgtcgagctc atcgagatcg agcgcgtcct cgtcgcgggc cggctcgtcc
      121 gacagcttgc cgtagcccag gtggcggatg gtgttgcgca tgcgatcggc caggaacagc
      181 gccagcgcct tgtagaagcg cgccgcgaat tcgctgtcgc tcttgagccg cttgtcgagg
      241 atggtcttgg cgctcttgcg cacatcgatg aaggccgcgg tgtagaccgc atgcgtatgc
      301 gtgaagtgtc cgttgtattc cggctgatac tgctgtctgt ggcggttgat gaactccggg
      361 ttcgggaaaa aaccaacggc actttcaaga ccgccagatg tcggcgctcc ctccgctcgc
      421 tgagcgaact ttccgatgtc gtgcaagaaa cccgctaagg caatcttata tacggtgtta
      481 tccatggtta ctcccacagg aatataggtt ccgaatcagg gtgttctgtt gaacacgcgt
      541 caccttcagg cacagcaata cgttgaattc gataccgacc ctgaccaaag ctgatcccgc
      601 gaccgacgtg aaagatctcg ccggcacgca acagcggcag aaacggggca agccttccac
      661 gatagacaac gttcccgacc attccacccc aataagaacg ctgatgctgt cgctgcgagt
      721 atcgttccca gcgcacaaat cgaccgttga cgtcgaccgt ctgaacagtc cgagcctgct
      781 ccatgatgcc cgtgaaatcc cactggggtg caggaatacc gctgacaacg cacagggacg
      841 tcagacgtcg cagaatggat ccgatgaggc gcgagaaatc aggaagattg ctgacctttc
      901 ccgtctgctt gtctttgaat gaaaccgggg tgagaaaacg aagttcaaca tctctcgtct
      961 cgtccgcttc gctcaccaga ggcggaacca ggctcaacac atctgggaca atcggctcca
     1021 gacgttcgac gcctcgtttg tacaccatgg gcgacaactc gcccagcatc cggacttcat
     1081 cgaggggcgc tcggatctgt tcacgggtaa acccgtgttg tccgagttct ttcaaggcga
     1141 aaatcaggta gggaatggcc tctgacgcac gaccaaagag agtaatgatg aacataaacg
     1201 tatcgccggg tgccaaacag atcgggccgg cgaactcagc ggcgaaatgc atcggccgcg
     1261 gcgtctcacg tgctccgcct gtcgccaact cggcctccgt gcctggtgcc gcggtctcca
     1321 tgagcagggc atagacgcag ttcttttcga gcagacaacc ggcgcatgcg acaagctcgc
     1381 cttcggctgt tctggctcga tgaaaacaca cggctcgctt gagatgaaaa cccaaccctc
     1441 cgcgcaacgt gttgaccggg ttctgcggaa acctgatctc gctctcggtc cgaaacgatg
     1501 cgcgtagcac acaatacggc aggctcacgg cacaagcatg tttcgagttc gagcatggtt
     1561 gcgccgggcg cacggaactc tcgttgatca tcgactgcat cgcgatggtg ttcatctcaa
     1621 atctttcctt acgacacatt ttatgaggca tcgcacaggt aaagccatct caccgaaccc
     1681 ggttcaaaaa atctgaaacc gcagtgtaaa atgattatat tacgtctcgt accgaatgca
     1741 accgttgagt aaacattgcg gatttttttc gtcgtttgag gccctctaac cgctgaacag
     1801 atcacaatgc gacaatgtcg gatcagacct gctcacttca ttcactgagg gggtctggtg
     1861 gttgagtgtg aagcgagcat gtgttcgatg agcttgtaca attccgcatc atcgagcagg
     1921 tgtttaccgc cgagacgcca ggtgtcattc agccgttcag ggtttccggt aaacggaagt
     1981 tgtaccagga cgggaaccgt gaacctgccg aacatggccg ccgttgcggc ggtttcgtcg
     2041 gcgtgcgcgt tcgtgctttc gatcttgccg cttttgcagg aaacgaccat gtaccggtgt
     2101 ccgaagcgca agacgacgtc cagatctgat ttgaaagaca catacggctt gtcgatgtca
     2161 gacagctcgt cacctcgaca ggctacggag tcactttcat gccgccattg tgttcgcagg
     2221 cgaaccgcga cctcatcagc cttccctgcg acgaaaaatc tccacccgac ccaccgttca
     2281 aaccactggt cctgacgtag atctattctg acctcagctt tggaagtctt ggagcggaga
     2341 aatgcatcgt ttccggtcac gtggcatgtc atttcgccgt attccgcatt ctgacccgct
     2401 gaaaaacgct gtaacgggtt cccgctttcc gagactctcc tgaaccagtt ggtaagcgca
     2461 tccatgatcc actttcgcct ggccagcaca ccttcatctt cacccgcttg gtgaatgttt
     2521 tctccacgta acgtcagcag tttcagcggc gaaggcccct tgaggggtcg atcgggaaga
     2581 tgaccggtca gatccgacag acgacgggtt gcattgttga tggtccagac actgaccact
     2641 tccggattgc tcattcgccc aagaagaatc agcagagctc cctgtaactt tgtaccgggt
     2701 gttgaattga cttcgagcct ctccgcgcga atttcggctt cgagcagttg aaggtcaaga
     2761 aaccctgtcg aaatgaaggc aacgtatgaa acgcccagct tcacccattc cttcatccag
     2821 agttcagccg tctgtacgat ctgagtgttg ccggcatcat aaaacagata caggtttgat
     2881 ggcgcgtggc tgcgaatcgc aatcaaggtc ggcagaatgt ccgagccgag aaacaccgcc
     2941 agagatgtcc cctgttgatt cctcttgccg cgttgttcca cgttgcgtgt ggaagccgac
     3001 cgaacggaat gcaccggctg cggtcgagcg aggcttctca cccaaccaga gaagttcctg
     3061 taggttgcgc gaatccgttc caagggccat cgctcgacgc gtatttttcc gcgcccctcg
     3121 gtttcgagtc tctcctgaac gttcttcctc gcggtaacaa cgatcacttc ccgatggaac
     3181 aactgtgcga agcgcgatcg accggttgca agcgtcatga tgtctcgcgt cttacgcagt
     3241 aacgcctctt tcaccgtgtc accgggttct agaaagggac gttccagaaa cacatagaaa
     3301 cgcagggtgt tctcgatatt gaccaccgcg tcaaatctca ccttcccaat cttcactccg
     3361 acgctgacac gctcattttg tccggtcgcg gcttcaccct caagtggcga aaaagccacc
     3421 ctttgaagtc tcagaacatc gagatgctcg gagcacagag gaagatcttc cgtatgaact
     3481 ttccgctttg aatcaatgcg aaagagcgtc attcgatcgc tgtcggagta gagaaaacgt
     3541 gtacgttcga gtggtgcttg cagcagcccg caggcgatct gaaaattcat cccgccattg
     3601 agattgaaga caagagtgcc ttcactgctc atgatgcgtc gaatcatttc catttctttc
     3661 ggtccgcgct catctcgagt acgattgtcc gattcaatct ccgtgatagt acaaacctct
     3721 atccaggtgg ttgagtagtc ggcgatctga cgggccacgt tcttcgtaaa tggtgtcgcc
     3781 agcagatcga tccgctcgaa agcacaacct ctgcgttgca gggccagcaa ggggacgagc
     3841 acggccgcca cctgagagcc gacgaaggaa acaaaatgga gcggtgatgt tgtcttatgc
     3901 aatgagcact gaatgtcggt attcatacgc gaagtgtatc acggtaagcc gtactggttc
     3961 ccgaggcgga actattgaga atcatttcat cctctcactc cttcggcacg gcgtgtcgcg
     4021 gcaggcgcac gccgtagcgg gtgttgccgt agcgaccgat ggggacgatg agcaggcggt
     4081 cgagcatggg gtgatcgcag agcgaggcgc gcaggcgacg gttgatcttg ctgatgtact
     4141 ggcgcatcgc ctcggtttcg aggccgtttt cgaggcgggc gagcatgtct tcctcgcgct
     4201 ggccgcccca gccgtaggag cggcggtaga actcggcaac ccggtgcggg aaaccgttgc
     4261 ggcacatgtc agcaatcgag agaaagcatc ggaacgcgca gtctccgtgc tctgcccgac
     4321 acgatgtttc ggagcacgaa cgcttgttcg acagaaaacc cgcgtaaatc gccagtaaca
     4381 atggctcgag cgtcgtcaca ctcgtaccaa cgcgtagatg acgcgccgta aaatccaagt
     4441 taacaggcca aggctctggc atttcaacag catatcctgc agtcggaacg tacagatcgt
     4501 agattttcat aaacgcctct cttcgcgccg ggtcggtcag cagttccaga aaccgacgac
     4561 gcccctcttc gccgtgaaag tcgaaggtca gcagttcgag cccgttttca caggcctgtt
     4621 ggcgcacgga ttcaagaaaa gaaacgattc gcggtgacgg ctgcaacacg tacatcaaca
     4681 gttgcggc
//
LOCUS       DAORHD010000197         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19295_length_4687_cov_2.733377, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000197 DAORHD010000000
VERSION     DAORHD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19295_length_4687_cov_2.733377"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..3095
                     /locus_tag="PKO06_05550"
     CDS             207..3095
                     /locus_tag="PKO06_05550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69143.1"
                     /translation="MAFFLRRLLILIVFAIFPVLVVQVAYRQIEHLEVEKLSEELTKK
                     QLRVLGVLKQNDSEPIMISRSFQRVFATIKNLPFTRQKKIVKNLYRIFPRAFDAYFFD
                     ANGRLIKDLSSQRHARRAVEMSYQALRDIAAGRKLAPEIEGLMKSVYNLTNVGSLAGS
                     KQKAFFMGNREFNSWTIHDFQKTENGEGIQGYFALIHHSALPKDYSLGLSIRAFNRHS
                     PMHQAGWANTRGGLVEVYPDAFESKPGFRAAFMRALNQYDMQFDDGENVVSMVMRSSG
                     GYCFISSPRPRFFPDGLKHAFHVVCVFWIFLVLYKIVDGGSGLVLRIPVRLLALFFFA
                     VGTPSLVLLVGGFYALKDHSHVRQQQMEKLVEEKVQRFDQRYPLFLGDLAYRLRQVVL
                     AARSQTDQQKRISIYQKFEDEKAMDFVVVVDHRGREVFKTKRFSSLMSQNQTFQQRGQ
                     FALLISRELLKKLNGSMKIDAGSLVMEATSGVLNTLVGDASFSFDGLLATMGEMFDMS
                     LAGESSFIFVDAVQITGSENVEEMVSGFCSGIQQEAIYLEKHLSQLNKQPDLKWRVHA
                     WGDYVGGRMVEKRNYRQAVVMQSDDAKMVDEIGQNYLRTKNHTRQVVRRNGSDELWMT
                     FRGKHLTKYTLIAAVSLQDLQQTVRTLWFALVVTAVFVLLSTLIIGMFLSEQFLKPIE
                     DLNQGIRSIEERRFEYRVPIHQHDELGDMAGLMNTVIEGMKDLQVARIVQENLFPQSA
                     LSLGSYQIYGKSRAMADIGGDYFDYFAVGGTQIVGLVGDVSGHGVSAALIMGMAKCNF
                     AMDEHQSRSLLENLTSFNSFLLKTVQKKKMMTMFHFGVNIERHRMTFINAGHNFPMYY
                     DAVKKSVRMLEQPALPLGMMKKVTYKPLELDLQPGDAVLFYTDGLVEAKDRNGEVIGY
                     ETAQQWLAECAAAGAEGIVDSLFARFDQVTTGQEAEDDVSIICLKRSC"
     gene            3116..4261
                     /locus_tag="PKO06_05555"
     CDS             3116..4261
                     /locus_tag="PKO06_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV69144.1"
                     /translation="MYFLIYGGMNAVFWLQIRKACALATTGTVGVSLWLLLMIFAPFL
                     TRLLIRYDWLQIASLSAYVGFTWMGLVFSFLILTAILYLLMIPVSLVGACFKRPVGLD
                     VVARPIFWIGCVYMAVVFVVGYLHADQLETVELEVPLNRAIQQFQPVRIVQISDVHLG
                     LTTINQRIRTLTQTAKSLRPDLLVVTGDLIDGPMSPEQEETLAAWFREIEAPLGRFFI
                     LGNHEMYEGIKHSERFAKAAGLRLLRNEIVQVTDWLEVAGIDDPTLLERSGQKTLRDV
                     ELLPTRSAERARLYLKHRPIVEAEAVDRFDLQLSGHTHSGQIFPFKYVTEKFTSHPCG
                     LHRVGSAWFYHSRGSGTWGPPIRFMNPPEVTLLHLTRASETAAIAAD"
BASE COUNT         1065 a         1143 c         1287 g         1192 t
ORIGIN      
        1 tagctcgttt cgaaatgtcc tggcgacgac atcgctgaag gtcctgttga tgcttgtgat
       61 gtgagaaaca aaccagcaga agaaacaaat cgcagaacgg tgagtggcga agtcagaccg
      121 aacaagaatt catgaaagat cgcgttttct ttttgcagcg ttcacggtaa gctgattggt
      181 acaattcgag aagtcggagc ggggcaatgg ctttttttct cagacgattg ctgattctca
      241 tcgtctttgc catttttccc gtgcttgttg tacaagtcgc ctatcggcaa atcgagcatc
      301 tagaagtcga aaaactctcc gaggaactga ctaagaaaca gctgcgcgtt ctcggagttc
      361 tgaaacagaa cgatagcgag ccaatcatga tttctcgatc gttccaacgt gtgtttgcca
      421 cgatcaagaa tcttccgttt acgcgccaga agaagatcgt gaaaaacttg tatcgcattt
      481 tcccaagagc tttcgacgca tacttttttg atgcaaacgg aagattaatc aaggatctca
      541 gctctcaacg acatgctcgc cgtgcggtgg aaatgtccta ccaggcgctt cgtgacattg
      601 ccgcgggcag gaaactggca ccggagatcg aagggctcat gaagagtgtc tataacctca
      661 caaatgttgg gagtcttgcg ggtagtaaac agaaagcctt cttcatggga aatcgtgaat
      721 tcaattcctg gactatccat gattttcaga aaacggagaa cggggagggg atacaaggat
      781 acttcgcttt gattcaccac tccgcgctac cgaaggatta ctccctgggc ctttccattc
      841 gagcctttaa tcgacacagt ccaatgcatc aggcagggtg ggcgaacact agaggaggcc
      901 tggttgaggt atatcccgac gccttcgaga gtaaaccggg tttccgtgcc gcgttcatgc
      961 gcgctctcaa tcagtatgac atgcaatttg atgatggtga gaacgtcgtg tccatggtca
     1021 tgcgcagtag tggcgggtac tgcttcatca gcagtccgag gccacgcttt tttcctgacg
     1081 gtctgaaaca tgcttttcat gttgtctgtg tcttctggat ttttctcgtc ctgtataaga
     1141 tcgtcgatgg cgggtctggt ctggttttac gaattccagt tcggttgctg gctctctttt
     1201 tctttgcagt ggggacccca tctctcgtct tgctcgtcgg tggcttttac gccctgaagg
     1261 atcacagcca cgtccgacag caacagatgg aaaaactggt cgaagagaag gtgcagcgat
     1321 ttgatcagag atatccgctt tttctcggcg atcttgccta tagactaaga caggtcgtcc
     1381 tagccgcaag aagtcaaacc gaccaacaga agagaatcag catctatcag aaattcgaag
     1441 atgaaaaagc catggacttt gtcgtcgtcg tcgatcatcg cggcagagag gtgttcaaga
     1501 caaaacgttt ctccagcctg atgtcacaga atcaaacgtt tcagcagcgc gggcagtttg
     1561 ccttgctcat ttcacgcgaa cttctcaaga agctgaacgg ttcaatgaaa attgatgcgg
     1621 gaagcctcgt catggaggct acgagcggcg ttctcaacac tctcgtcggt gatgcgagtt
     1681 tttcttttga tggattgctg gcaacgatgg gtgaaatgtt tgatatgagt ctggcggggg
     1741 agagctcgtt tatttttgtt gatgcggtcc agataaccgg atcggagaat gtcgaagaga
     1801 tggtgtcagg cttttgcagc ggtattcagc aggaagcgat ttatcttgaa aaacatctct
     1861 cgcagctgaa caagcagcct gatttgaagt ggagagtcca tgcctggggt gattacgtgg
     1921 gcggcaggat ggtcgaaaaa cgtaactatc gacaggccgt cgttatgcag tctgatgatg
     1981 caaagatggt tgacgagatc ggacaaaact atcttcgcac gaagaaccac acgcgccagg
     2041 ttgtgcggcg aaatggctca gatgaactct ggatgacgtt tcgcggtaag catctgacga
     2101 agtacaccct gatcgctgcc gtctcactgc aagatctgca gcagactgta cgtacccttt
     2161 ggtttgctct cgtggttacc gctgtgttcg tgcttctttc gaccctgatc attggaatgt
     2221 ttctctcaga acagtttctc aaaccgatcg aagatctgaa ccagggtatt cgctcgatcg
     2281 aagaacgtcg atttgaatac cgggtaccga ttcaccagca cgacgaactc ggcgacatgg
     2341 caggtctgat gaatacggtg atcgaaggca tgaaggatct gcaggtcgcc cgcatcgtgc
     2401 aggaaaacct gtttccacag agcgctctgt ctctcgggtc ctatcagatc tatggaaaaa
     2461 gccgtgccat ggccgatatt ggcggtgact attttgatta ctttgccgtg ggcggcacgc
     2521 agattgtcgg gctggtcgga gatgtatcag gtcacggtgt ttcggccgcg ttgatcatgg
     2581 gtatggcaaa gtgcaacttc gccatggatg aacatcagtc acgaagcctg ctcgagaacc
     2641 tgacgagttt caattccttt cttctgaaga cggtccaaaa gaagaaaatg atgaccatgt
     2701 tccatttcgg agtgaacatc gagcggcatc gcatgacttt tatcaacgcg ggacacaatt
     2761 ttccgatgta ttacgatgca gtgaagaaga gcgtgcgcat gctcgagcaa cccgctttgc
     2821 ctctgggcat gatgaagaag gtcacgtaca aaccacttga acttgatttg cagcctggtg
     2881 atgccgtgtt gttctacacc gacggactgg ttgaagccaa agatcgcaac ggtgaggtga
     2941 tcgggtatga gacggctcag caatggctgg cggaatgtgc cgccgctggt gcagagggca
     3001 tcgtcgacag tctctttgcc cgttttgacc aggtgacgac cggacaggaa gcggaagatg
     3061 atgtttccat catctgtctc aaacgaagct gctgaccatg aggatgtttc tcgcaatgta
     3121 tttcttgatc tatggtggta tgaacgcggt attctggttg cagatcagaa aagcgtgtgc
     3181 tctcgctaca accggtactg ttggcgtttc tctgtggctg ctgctgatga tattcgctcc
     3241 atttctcacg cggcttctga ttcgttacga ctggttgcag atcgcttcgc tgagcgccta
     3301 tgtcggtttc acctggatgg ggcttgtttt cagttttctt atccttactg ccatactgta
     3361 tttgctcatg ataccggtct cgctcgtcgg ggcatgcttc aaaagacccg tgggtctcga
     3421 tgtggtggcg cgaccgatat tctggatcgg gtgtgtctac atggcggtcg ttttcgtcgt
     3481 cggatacctg catgcggatc agcttgaaac ggtagagctg gaagtgcctt tgaatcgagc
     3541 gattcaacag tttcaacccg tacgtatcgt gcagatttcc gatgttcacc tcgggctgac
     3601 gacgatcaat cagcgcatac gcactctcac gcagacagcc aaatcactcc gccccgatct
     3661 cctggtcgtc acgggcgacc tgatcgacgg cccgatgtcg ccggaacagg aagagactct
     3721 ggccgcctgg tttcgcgaga tagaagcgcc gcttggcagg tttttcattc tcggcaacca
     3781 cgaaatgtac gagggaatta aacattctga gcgatttgcc aaagctgcgg ggctcaggct
     3841 gttgcggaat gagatcgtcc aggttactga ttggctcgaa gtggccggaa ttgacgatcc
     3901 gaccctgctc gaacgcagcg ggcagaagac gcttcgcgat gtcgagttgc tgccgacacg
     3961 ttcggcggag cgcgcgcgac tctatctgaa acatcgaccg atcgttgaag ctgaggccgt
     4021 cgatcgtttt gatctgcagc tctcgggaca cacacattcc ggccagatct ttccgttcaa
     4081 atacgtgacc gaaaaattca cctcacaccc gtgcggtttg catcgcgtcg gttcagcgtg
     4141 gttctaccat tcgcgcggca gcggtacgtg ggggccgccg atccgcttta tgaacccgcc
     4201 cgaagtgact ctgctgcatc tgactcgggc ttccgaaacc gccgcgatcg cggccgactg
     4261 acccaagggc ttgccaggtt cactgccgcg tgacaaggat ttctgttccg ttgatggtga
     4321 ggtgcgctgc acgtaacatg tgatactttg cccgactcat gccccggcgc aggcgatggc
     4381 gttcagtttt gtctcggcgg gtgattctcg ttgagcttat tgttatcagt gtgagagggt
     4441 ttccgcagat atgataaact ggtttttcat cttcgagttg gaggttcacc catgcagaca
     4501 tccatcacaa gtgcggtgaa aggtcttctc ctttcggtca gtctgtgttt cgtctgccag
     4561 aacagcccgg ttctggctgc taccccatat gcagatgccc caatgcaggt tcaaaggggt
     4621 ggagaaatgc aggcgccggc taccccaacg actacgtccg gcggaacagc acagagtgtg
     4681 tcggtcg
//
LOCUS       DAORHD010000198         4682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19321_length_4682_cov_2.682516, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000198 DAORHD010000000
VERSION     DAORHD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19321_length_4682_cov_2.682516"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PKO06_05560"
     CDS             complement(<1..1697)
                     /locus_tag="PKO06_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNV69145.1"
                     /translation="MIMLGLYSLMAKFLYFWLVSWTMMQNESAARVVPPTVFTIACLI
                     DLGVLFLLFRGKSYRAARVGGLLAFNLVFGIFCLGTIMFYRLFGVPLSLDVMGFGQDV
                     TEIGDSVRSLLEPMDGTLFAFDLVSMLILVIPAWRRRFAASDEFFCTISTKRIVVAIL
                     LLAALWVPFAEPRHSFFEPFTRRDLEGVWTFNPLNYFLLEVATSLKETCFPSQPDPLV
                     VENIRREIQARKERQSQVPKRFPERREPLNVILIQMESVMFEVLGAQLQGQSVTPNLD
                     AMAREGVLLPSYYSPAISTADSEFSALTSLYPISNKTVYLNLAHHRFESLPKILGRSG
                     YATFVTNGSLGRAWNVRNMHKRLGFQHQAYFDEYRSGRMTGPWLADSDLLERVSKRVM
                     HLSQPFFAFVMLISSHHPFTLTGLPETFAVPAEASAEEKEWIRYCNAVHYTDSAIGEF
                     LARLRQQGLLERTLVVAYGDHPIMLKRQFKTLRDLYGGLPSGEKIMRMMNSRVPGIIY
                     APGFLSAGTIDRVCSHVDLAPTILELCGLPPSPVFLGSSVFAEGPGSTGHKYWVAISS
                     "
     gene            complement(1753..3369)
                     /locus_tag="PKO06_05565"
     CDS             complement(1753..3369)
                     /locus_tag="PKO06_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69146.1"
                     /translation="MTEENRASADSHSNLSLFVKSIGAALLAAAVVFGFTARFSGYLV
                     DDAYIGFQYLRNVCLGHGFVFCAGQPPVEGVTNLGWLLLLLPWGSSPMLSSVAQWIGA
                     GFAWGAILLTLYIRVRQQEHAENESAIVVFAPSLLLLFSSDFLYFSSGGMETGFMAFC
                     VLLPVVWLSADLSCTWKLGLFGGLLTLVRPESVLILPGFLLVRRVWGRGSEGSGAANQ
                     SAASDSFSGMFIAWTLFLVLCALLRLWYFGMPLPNTFWSKPGSVTLLAGQLINSFLGV
                     HSNLTFPFSTTFIFIVPVILAGWWYRRGQSSARRTDWLLAVTMTGLVFAVSAPLDWTK
                     TGRYFAPYLGAALLLLWDGLTVLTRSVLSWFKMPKASSTTLLLLVELLLLTAVCDFGI
                     RFSESRLAQYPGYVLVSRPLFPGAQWLAENTAPNAVVASRRIGVLALVSGRRVFDYLN
                     GLTEPEVARLVSRRRQPLSGPHDPDLKGIWLRVNPDYVLEDESEMQKIARECGGTVER
                     MVIHGTPYVQCQRFSLGAQDWVLLRRSSSE"
     gene            complement(3620..3802)
                     /locus_tag="PKO06_05570"
     CDS             complement(3620..3802)
                     /locus_tag="PKO06_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNV69147.1"
                     /translation="MRKTSKKGQGLVEYVLIISLIAVVCIALLSSVGETVRDGLWDKV
                     ADKLQTTSSNLESSGS"
     gene            4010..>4682
                     /locus_tag="PKO06_05575"
     CDS             4010..>4682
                     /locus_tag="PKO06_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HNV69148.1"
                     /translation="MDWLPHLRDVCLQRLSHVKAASHAWPYYPHAEPSTEATLFATLA
                     LVVHGLPSELWEPSWKWVVTLQNPDGSIGVGPHARREGLWLTALLLIVAVQVSDKAVA
                     DRATRFLEAFQSKAMPQNPQVQQNNSIIGWPWSADTFGWVEPTAWAVLALQAAGQGAC
                     SRAVEGRRLLLDRMLPSGGWNYGNRVVHDQELLPFADTTALALLALHGNSTLDTLKPS
                     IDLLDS"
BASE COUNT         1171 a         1376 c         1243 g          892 t
ORIGIN      
        1 gaactgattg ccacccagta tttgtggcca gtcgatcctg gaccctcggc aaaaacactc
       61 gagccgagaa acaccggcga gggcggcaga ccacacagtt cgagaatcgt cggcgccaaa
      121 tcgacgtgac tgcacacgcg atctattgta ccagctgaca gaaagccagg agcatatata
      181 atgccgggaa cgcggctgtt catcatgcgc atgatttttt cccccgatgg caacccgccg
      241 tacagatctc tcagcgtctt gaactgccgc ttcaacatga tcggatgatc gccataggcc
      301 accaccagcg tgcgttccag aagcccctgt tgccgcaacc gcgccagaaa ttcaccgatc
      361 gccgagtcgg tgtaatggac ggcgttacaa tagcggatcc attctttttc ttctgccgaa
      421 gcttcggccg gcaccgcaaa cgtctccgga agccccgtga gcgtgaacgg gtgatgtgag
      481 ctgatgagca tgacgaaggc gaagaacggc tgactgaggt gcatcactct tttcgatacg
      541 cgttcaagaa gatcagaatc ggcaagccag ggaccggtca tacgacccga ccggtattcg
      601 tcaaaatagg cctgatgctg aaaaccaagt ctcttgtgca tgttccggac attccaggct
      661 cgacctaaac tgccgttcgt cacgaaagtc gcgtatccgc ttctcccaag aatcttgggc
      721 aatgattcga agcgatggtg agccaggttg agatagacgg tcttgttact gatcggatac
      781 agcgacgtaa gggccgagaa ctcagaatca gccgtggaaa tggctggaga atagtaactc
      841 ggcagcaaca ccccttcacg ggccatggca tcgaggttcg gcgtcacgct ttgcccctgt
      901 agttgcgctc cgagaacctc aaacatgacc gattccatct gtatcagaat cacgttcagc
      961 ggttcgcgcc tctctggaaa tcgtttcggc acttgacttt gtcgctcttt tcttgcctga
     1021 atttcgcgac ggatgttttc gacaaccaac ggatccggct ggctggggaa acaggtttct
     1081 ttcaggctcg tggcgacctc gagcagaaag taattcaacg ggttgaaagt ccagactccc
     1141 tcgaggtctc ggcgggtaaa cggctcaaag aagctgtgac gaggttcggc aaacggcacc
     1201 caaagagcag ccagaagcag aatcgccacc acgatgcgct tcgtggagat cgtgcaaaaa
     1261 aactcatccg aggccgcaaa acgacgtcgc catgctggaa tgacaagaat caacatggac
     1321 acgagatcaa aagcgaagag agtgccatcc atcggttcaa gcagcgaccg caccgagtca
     1381 ccgatctcgg tgacatcctg tccgaatccc atgacgtcaa gggatagcgg aactccgaaa
     1441 agccggtaga acatgatcgt gccgaggcag aagattccaa acaccagatt gaacgccaga
     1501 agcccgccca ctcgcgcagc ccgatagctt tttccgcgaa acagcagaaa aagcacgccc
     1561 aggtcaatca gacaggcaat ggtgaacacc gttggcggaa caacgcgtgc ggcggattcg
     1621 ttctgcatca tggtccagga aaccagccag aagtacagga acttcgccat cagcgagtat
     1681 aaaccaagca tgatcatacg ttgtattctc gtgtttttgt cggacgctca ggcgagacgc
     1741 cgacacggct catcattcgg atgatgaacg gcgcagcagc acccaatcct gcgctccgag
     1801 tgagaaacgt tggcactgca catacggagt gccatgaata accattctct cgaccgtgcc
     1861 cccacactct ctggcgattt tctgcatctc tgactcatct tcaagcacat agtccgggtt
     1921 gactcgcaac cagatgcctt tcagatcagg gtcatgaggc ccggaaagcg gttgccgacg
     1981 acgcgagacc aaccgcgcca cttccggttc cgtgagaccg ttgagatagt cgaaaacccg
     2041 tcgcccggaa accaacgcga gaaccccgat acgccgtgac gccacgaccg cgtttggcgc
     2101 tgtgttctca gcgagccact gcgccccggg aaacagaggg cgggatacga gcacataccc
     2161 gggatactgc gccaatctcg attcagaaaa cctgatgccg aaatcgcaga ccgcggtcaa
     2221 gagcagcaac tcaaccagca acagcagggt tgtcgaggag gctttcggca ttttgaacca
     2281 ggagagaacg ctgcgcgtca gcacggtaag tccatcccac aggagcagaa gcgccgcacc
     2341 gagatatggc gcgaagtatc ttccggtttt tgtccaatcg agcggcgccg acacggcaaa
     2401 caccaacccc gtcatcgtca ccgccagtag ccagtccgtc cgccgcgctg acgattgccc
     2461 gcgtcggtac caccagcccg ccagaatgac aggcacgatg aaaatgaagg tggtcgagaa
     2521 tgggaaggtg agattgctgt gaaccccaag gaaactattg atcagttgcc ccgccagcaa
     2581 ggtcaccgaa ccgggcttgg accagaacgt gttcggcaac ggcattccaa aataccagag
     2641 acgcaggagc gcacatagca ccagaaacag cgtccaggcg atgaacattc cagagaacga
     2701 atcagaggca gccgattgat tcgctgcacc ggatccttcc gaccctctgc cccaaaccct
     2761 tctgaccagc aggaagcctg ggagaatcag cacgctttca ggacgcacga gcgttaaaag
     2821 ccccccgaac aaaccgagct tccaggtgca tgacagatct gccgacagcc agactaccgg
     2881 caacagcaca caaaacgcca tgaaacccgt ttccatgccg ccgctggaga aatacagaaa
     2941 atcagatgaa aacaacaaca gcaacgacgg ggcgaacacc actatcgcac tctcgttttc
     3001 cgcgtgttcc tgttgtcgca cgcgaatata gagagtcagc agaattgccc cccaggcgaa
     3061 gcccgctcca atccactggg caaccgaaga cagcatcgga gagctgcccc acggcagcag
     3121 cagaagcagc cagccaagat tcgtcacccc ttcgaccggg ggttgtccgg cacaaaacac
     3181 gaacccgtgc ccgagacaga cgtttctgag gtattggaat cctatgtagg catcatcaac
     3241 gagatacccg gaaaaacgag ccgtgaagcc aaaaacgacg gctgcggcca acagcgccgc
     3301 accgatcgat ttcacgaaca gcgacaggtt ggaatgggag tcagcggatg cgcggttttc
     3361 ttcagtcact tgagcacgca tagttgtgat gtctgaaaag aagccattac tgtagaaatc
     3421 gaactaggac tctcaccttc tctggcgagc gagagttgtg caggtgctga gatgaagccg
     3481 ggactacgtg aatggaacga gtcacacaac aaaccccgag tggaaaagcg ctctcgcgtc
     3541 accaacggac aagcatccga tggcattgcg cgctgctcgc cacattcacc accgatcgct
     3601 ttccgcgaaa caggagctgt caggacccgg aactctccag gttcgagctg gtcgtctgca
     3661 gtttgtctgc cactttgtcc cacaatccgt ctctgacagt ctcaccgacg ctggagagca
     3721 aggcaataca aacaacggca atcaggctga tgatcagcac gtactcgaca agtccctgac
     3781 ccttttttga agtcttgcgc atagtttcaa tcctttatca tccaggaagt agcaatttga
     3841 atgccatccg ggtcactcat tgtcctcccc gatgagagat ctatcatcac gcagaatacc
     3901 atcattgatc tcagccttcc actctgtttg cgtgcttctc ttgtcagtgc cgctcctgtt
     3961 cgagtatcgt acccgaggcg aaccgcaaac ctcaatcggg aaaaggcctg tggactggtt
     4021 acctcatctg cgcgatgtct gtctgcaaag actgtcgcac gtcaaggcgg caagccatgc
     4081 gtggccttat tatccccatg cagagccatc aactgaagca accctgtttg ccactctcgc
     4141 gctggtcgtt catggccttc cttccgaact ctgggaacca tcctggaaat gggtcgtcac
     4201 cctgcagaat ccagatggat cgatcggtgt cgggccccat gccaggcgtg agggtctctg
     4261 gttgaccgct ttgctgctga ttgtcgcggt acaggtgagc gacaaggcgg tcgcggaccg
     4321 ggcaaccagg tttctcgagg cgttccagtc aaaagcgatg ccgcaaaacc cgcaggttca
     4381 acagaacaac tcgatcatcg ggtggccctg gtcggcagac accttcggct gggtagagcc
     4441 cacggcatgg gcggttctcg cgcttcaggc cgcggggcaa ggcgcgtgtt cgcgagccgt
     4501 cgaaggacgc agacttcttc tcgatcgtat gcttccgtct ggtggctgga actacggaaa
     4561 ccgagtcgtt catgaccagg agctgctccc gtttgcggac acgacagctc tcgcgcttct
     4621 ggcccttcac gggaacagca ctctggacac tctgaaaccc agcattgatc tgctcgacag
     4681 ta
//
LOCUS       DAORHD010000199         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19357_length_4675_cov_3.345671, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000199 DAORHD010000000
VERSION     DAORHD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19357_length_4675_cov_3.345671"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKO06_05580"
     CDS             complement(<1..377)
                     /locus_tag="PKO06_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69149.1"
                     /translation="MQLLESLTEWALRFTRPDVIELGTSQFRLLRGTVQNGLVSIRRM
                     HQVPAPKGLVLSSHLNQAVFDLEDFQRACRDLFTPEPPRGRHVSVVLPDTAFHFATMT
                     FPTPLFRANPLSLIEREIQSSAPL"
     gene            complement(356..2110)
                     /locus_tag="PKO06_05585"
     CDS             complement(356..2110)
                     /locus_tag="PKO06_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV69150.1"
                     /translation="MAKILTQAANLRKPFDMVLEQRQLLTREQILRHQQEARRRQISL
                     EDLLCSEAVLPPDTVYESLADAFGLTWHSLEGCQIPDQLPARVIELCAKECLIPLTLQ
                     NGSLTVATFRPALQAAHASMLAAAGVRPVFQVCSPDRARPLLEALQKLTTPEEQDGGI
                     DSLISSLSGTGEDAKGAEGLEISGVSDSPEAPSVVDLVNRALTMAVRQKASDIHVEAT
                     EKGLRVRFRLDGVLVHRFELAGPPAAAFISRILVMAGLDITEKLLPQDGSFKVRFEGR
                     DVEFRIASIPGSYGQNLTLRLLSGQEVGAISLEKLGLTGLELEVLTNTIKFPHGMILV
                     AGPTGSGKSTTLYAILERLANPAVKILTIEDPIERRINLVTQVQVRINRNDPARSLTF
                     ARGLRTFLRLDPDIIMVGEIRDQETAEIAVQASLTGHLVLSTVHANSSTETLRRLQNI
                     GVDYFLFMSTLNLVIAQRLVRNLCPHCRTSRPPTPEESELFPAEIRPDILFQAHGCNR
                     CFGTGYAGRVGIFEFLPVDDEIRDSIQADNLMSCMPLVRRRTLKTLRQSALIHVADGA
                     TDLNELERVCGPCSFSKV"
     gene            2229..4475
                     /locus_tag="PKO06_05590"
     CDS             2229..4475
                     /locus_tag="PKO06_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69151.1"
                     /translation="MIPITNLDQRSPTRVRLWLVAGLLLLAMFTRVVNSGTVVGRSFQ
                     RLDLAPAGSQISGGADLGGFLLTFTGPVEAQVVRDPDIPLAYVDILGGKLEVSPVLMP
                     FPNGPARLVRLHQTQAQPPVVRATFFLRQRATPLLVPAAQGLMIGFSSKGSAPSLPSP
                     ADRITQTADGRPVPFPPMAPGTRAWRTPAPVAASEETARAPASIHLDVKGADVSTLLQ
                     ELARQSGMSLHFRDPFTGQIDLKVSGVAPLEAMSQIMTRVGGTLTIEDREIWISRRDN
                     PLLSFADDQFIEKANLQGLAVGDVLRALGQMGDLNIVLDRSVGQIKDQPVDVLFQKQT
                     YRRAFETLLNLYELDIRPVDSRTLLVLTKDAARTLAGRVVRIFPLNLPFTKMKDLIDR
                     SLSEDQKKRVTIQEDLGNLVAVGDHEVMSALETQVKSLEGKMERAGEGVVRVMFHPVN
                     TKPADLVKLVETALGDGNKPQMIVDERTDAIVIVGPKILMERAMSLLRNLDKTATRQA
                     IIQVKLLEISRRDLNKLGVRLGADKIGVADITQWTHTAIPADFIDSRDLTNARTLANP
                     TVRCMDKQEAKIDISEQIPVKNTVTEYLPIASASLAARTSDNWTTTDVGIKLTLKPFI
                     HQNRELSMEVNVDQTEVISFVEGRPWTSKRNIQTQVRMKDRETVVIGGLIKRKRSDNE
                     KPLPIVSRIPFLKRWFKRVEFKDRTHEESEMVVLITPRVIESVGLNQPDEQLTTTPIQ
                     VSVPRNSP"
     gene            4472..>4675
                     /locus_tag="PKO06_05595"
     CDS             4472..>4675
                     /locus_tag="PKO06_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69152.1"
                     /translation="MRGHRDTIGLVLIFFAAFSMGFAIESRWGEKRSLATRGDAALEV
                     ARVLNALQPLLRPWETRAGPLETF"
BASE COUNT         1000 a         1317 c         1460 g          898 t
ORIGIN      
        1 agaggcgcgg aagactggat ctcgcgttcg ataagtgaca gcggattggc gcgaaacagc
       61 ggcgtgggaa aggtcatggt cgcgaagtga aaggcggtgt cgggcaggac gaccgagaca
      121 tgccggccac gtggcggctc gggcgtgaag agatcccggc aggctcgctg aaagtcttcg
      181 agatcaaaaa ctgcctgatt gagatgactg gaaagtacaa gcccctttgg ggcaggaacc
      241 tgatgcatcc ttcgtattga taccaggcca ttctggacgg tgccgcgcag cagccggaac
      301 tggctggtac ccaactcgat gacatcgggt cgggtgaagc gcagagccca ttcggtcaga
      361 ctttcgagaa gctgcatggg ccgcagaccc tttccagttc gttgagatcc gttgctccgt
      421 cagcgacatg gatcagtgcc gactggcgca gtgtcttcag ggttcgccga cggacaagcg
      481 gcatgcagga catcagattg tcagcctgaa tgctgtctct gatctcatcg tcgacgggaa
      541 gaaactcgaa gatgccgaca cggcctgcat acccggtgcc gaaacatctg ttgcagccat
      601 gtgcctgaaa gagtatatcg gggcgtatct cagctggaaa cagctcggac tcttccgggg
      661 tcggtggtcg cgaggtacgg cagtgagggc agagattgcg taccagtcgt tgcgcgatca
      721 ccagattgag cgtactcatg aagagaaagt agtcgactcc gatgttctgc agtcgacgga
      781 gggtctcggt ggaagagttg gcgtgcacgg tcgacaggac cagatgcccg gtaagactcg
      841 cctgaactgc gatctcggcg gtctcctggt cgcggatttc gccgaccatg ataatgtcgg
      901 ggtcgaggcg cagaaacgtg cgcagaccgc gggcaaaggt cagcgagcgt gcggggtcat
      961 tgcggttgat gcgcacctga acctgggtga ccagattgat gcgccgctcg atcgggtcct
     1021 cgatcgtcag aattttcacc gccgggttgg cgagacgttc gagaattgca tacagggtgg
     1081 tcgatttccc gcttcccgtc ggaccggcca cgagaatcat cccatgggga aacttgatcg
     1141 tgttcgtgag cacctcgagt tcgagacccg tcaagccaag cttctcgaga ctgattgcgc
     1201 cgacctcctg accagaaagc agtcgcaggg tgaggttctg tccgtacgag ccgggaatgg
     1261 aggcaatgcg gaattcaacg tcgcggcctt cgaaacgaac cttgaaactc ccgtcctgcg
     1321 gcagcagctt ttcggtgatg tcgagccccg ccatgacgag aatgcgcgaa atgaacgccg
     1381 ccgcgggtgg gccggccaac tcgaagcggt ggaccagaac gccatcgaga cgaaacctca
     1441 cccggagccc tttttccgtg gcttcgacgt gaatgtcgct ggccttctgt cgcaccgcca
     1501 tggtcagagc tcgattgacc agatcgacca cggacggagc ttcgggagag tcagagacac
     1561 ccgagatctc gaggccttcc gcgcctttgg catcctcgcc agtgccgctc aggcttgaga
     1621 tcagagaatc gatgccgcca tcctgttctt ccggcgttgt gagtttctgc aatgcctcga
     1681 gcagagggcg ggcgcgatca ggcgaacaaa cctgaaagac cgggcgaact cccgctgccg
     1741 ccagcatact ggcgtgagcc gcctgcagcg ccgggcggaa cgtcgccacg gtcaagcttc
     1801 cgttctgcag agtcagcggg atgaggcact ctttggcgca gagttcgatg acacgggcgg
     1861 ggagctggtc tggaatctgg cagccttcca gagagtgcca ggtcaggccg aacgcgtcgg
     1921 caaggctttc gtagacggtg tcgggcggaa ggacggcttc gctgcacaac agatcttcaa
     1981 ggctgatctg gcgcctgcgg gcttcctgct ggtgtcgcag aatctgctcg cgggtgagca
     2041 gctgacgctg ctcgaggacc atgtcgaacg gcttgcgcag attcgcggcc tgagtaagga
     2101 tcttggccat agacggaatc tcccggcatt gactgcatga ctctctgaca ctatcgacct
     2161 gaatctgacg atggatgaga ctcaataatc acacgcaaca cgccgaaaga ggaagtggga
     2221 aaaaaggtat gataccgatc acgaacctcg accagcgctc accgaccaga gtccgcctct
     2281 ggctggtagc gggtttgctg ctgcttgcga tgttcacccg ggtggtgaac agcggcaccg
     2341 tggttggtcg ctcttttcag cgtctggatc tggcacctgc cgggagccag atcagcgggg
     2401 gagcggatct gggaggcttt ctgctgacct tcaccggccc ggtcgaggcg caggtggttc
     2461 gcgatccgga cattcctctg gcgtatgtcg atattctggg cggaaaactg gaagtcagcc
     2521 cggtgttgat gccgttcccg aacggcccgg cccgactggt gcgactgcat cagacccagg
     2581 cgcagccgcc tgtggtgcgg gccacgttct ttctgcggca gcgagcgacc ccgctgctgg
     2641 tgccggcggc acaggggctg atgatcggct tttcctccaa agggtccgcg ccgtcacttc
     2701 cgtcgcctgc cgaccgaatc acgcagaccg ccgacgggcg tccggtcccc tttccaccca
     2761 tggcacctgg cacgcgcgcc tggcgcacac ccgccccggt cgccgcttcc gaagagaccg
     2821 cccgtgcgcc ggcgtcgatt catctcgatg tcaaaggggc ggatgtgagc acgttgctgc
     2881 aggaactggc gcgtcagagc ggcatgagtc tccatttccg tgatccgttc accggtcaga
     2941 tcgacctcaa ggtatcgggg gttgcaccgc tcgaagcgat gtcgcagatc atgacccgcg
     3001 tcggtggaac attaacgatc gaggaccggg aaatctggat ctcacgccgg gacaatcctc
     3061 tcctctcgtt cgccgacgac cagttcatcg agaaggcgaa tctgcaggga cttgcggtgg
     3121 gcgatgtcct gcgagctctg gggcaaatgg gcgatctgaa catcgtactt gatcgttctg
     3181 tcgggcagat caaggatcag ccggtcgacg tgctctttca gaagcagacc taccgccgcg
     3241 ccttcgaaac gttactgaac ctgtatgaac tcgatattcg tcccgttgac agccgcaccc
     3301 tgcttgtatt aacgaaagat gccgctcgga cgcttgccgg aagagtcgtg cgtattttcc
     3361 cgctcaatct gccgttcacc aagatgaagg acctcatcga tcgttccctc agcgaagatc
     3421 agaaaaagcg agtcaccatt caggaagacc tcgggaacct ggtcgccgtg ggtgaccacg
     3481 aagtgatgag tgctctcgaa acgcaggtga aatcactcga agggaagatg gagagagcgg
     3541 gagagggagt ggtccgggtg atgtttcatc cggtgaacac gaagcccgct gatctggtga
     3601 aactggtcga gaccgctctg ggcgacggca ataagccgca aatgatcgtc gacgaacgaa
     3661 cggatgccat cgtgatcgtt gggccgaaga ttctcatgga aagagcgatg agcctgttgc
     3721 gcaacctgga caaaaccgcg acgcgccagg cgatcattca ggtcaagctg ctcgagatca
     3781 gtcgacggga tctgaacaag ttgggagtga gactgggagc ggacaagatc ggagtcgctg
     3841 atatcaccca gtggacgcac acggcgattc ccgcagactt catcgatagt cgcgacctga
     3901 cgaatgccag aaccctggcg aatccgaccg ttcggtgtat ggacaagcag gaagccaaaa
     3961 tcgacatctc agaacagatt ccggtgaaga atacggtcac cgagtatctg cccatcgcct
     4021 cggcatctct ggcggcgcgc acctcggaca actggacgac caccgacgtc ggtatcaaac
     4081 tgaccttgaa gccctttatt catcagaatc gtgaactgtc gatggaagtg aatgtcgatc
     4141 agaccgaagt catcagcttc gtcgaaggac gcccctggac gtcgaaacgc aacattcaga
     4201 cccaggtccg catgaaagat cgagagacgg tcgtgatcgg cggcctgatc aaacgcaagc
     4261 ggagcgacaa tgaaaagccg ttgccgatcg tgtcgcgcat tccgtttctc aagcgctggt
     4321 tcaaacgcgt tgaattcaag gatcgtacgc acgaagagtc ggaaatggtc gtgttgatca
     4381 cgccgcgtgt aatcgagagc gtgggactga accagcctga cgagcagtta accaccacac
     4441 ccatacaggt ttcggtgccc aggaactcgc catgagaggt catcgagata ccatcggact
     4501 ggtactcatc ttcttcgcgg ccttcagcat ggggtttgcc atcgagagtc gctgggggga
     4561 aaaacggtcg ctggcgacgc ggggtgatgc ggctctcgaa gtcgcgcgag tgctcaacgc
     4621 gctgcagcct ctgctgagac cctgggaaac ccgcgcgggt ccacttgaaa ccttt
//
LOCUS       DAORHD010000200         4675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19359_length_4675_cov_3.049134, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000200 DAORHD010000000
VERSION     DAORHD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19359_length_4675_cov_3.049134"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..722
                     /locus_tag="PKO06_05600"
     CDS             120..722
                     /locus_tag="PKO06_05600"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNV69153.1"
                     /translation="MGILVGKDAPDFVAKAIMGDNSINERFQLKSFLQGKYGILFFYP
                     LDFTFVCPSEILAFDHRLDEFRKRNTEIIGVSVDSEFSHLAWKNTPVEKGGLGWVRFP
                     MVSDLSKSISREYNVLINDSVALRATFLIDRQLKVRHQLVNDLPLGRNVDEALRMVDA
                     LQYFEKSGEVCPAGWNKGKPGMKATTDGVAEYLASHGKDL"
     gene            complement(828..1496)
                     /locus_tag="PKO06_05605"
     CDS             complement(828..1496)
                     /locus_tag="PKO06_05605"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNV69154.1"
                     /translation="MRDMPRPGLSYLLPVSRELYATRVYLRRFRHRDAQPLLDLLVRN
                     ESWLAMGLPPLPARFELSDMKQEILRRQRLERQGSRLDLAIFDKESHRLVGEVSLHSI
                     SWGIQLSGGLGFWLAESSAGNGLMKEALATMLSFVFEESPLHRIWAATTHCNVRTQEL
                     LRRLGFTHDGTIRQELFLAGTWQDMHHFSLLRDEYDNLAERWISKGYLGIHPNQKARH
                     GKQR"
     gene            1562..2257
                     /locus_tag="PKO06_05610"
     CDS             1562..2257
                     /locus_tag="PKO06_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNV69155.1"
                     /translation="MTLKAVFFDVGGTLIFPNPERIGATFAQTGSALPTERWLEAVHR
                     ASASLDAQLEAGKRMGDDWWQVYFGLVVSHATGRPAHEWSGFKTFLKQLKDDHVAHNL
                     WDSVAPGAPETVERLQMAGFSLGVISNSDGRVRYQLQQAKLAERFSFIIDSYEVGCDK
                     PDAKIFHLGLKHAGLKPQQVLYVGDFVEIDHRGATGVGMEAVIIDPLRLRRHLDGVRF
                     IDSISQLPSLLDL"
     gene            complement(2384..3832)
                     /locus_tag="PKO06_05615"
     CDS             complement(2384..3832)
                     /locus_tag="PKO06_05615"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV69156.1"
                     /translation="MDSLTFIVSSFSGALRLLIQADAEVLAILWLSLKIAVVSTVGAT
                     LVAIPIAWHIHLAGPRWRYLFSLLFQVLAAIPTVIIGTVCFLLLSRNGPLGALELLYT
                     PTAIIVGDLLLAIPLVALFLISALSLVPKGIFDTARNLGAHRLQLVRVVFNECREALL
                     IAVCTAFGRVVSEVGAAMILGGNIRGVSRTMTTAISLEIGKGETELSIALGLILLGAA
                     LANAFLIGHAQYFVEKFRHRDLTPVSSAPTSDTKSLFPANRTPPTARLTALSKSFANP
                     LFQNLTFELPLEGGTSLQGRSGCGKTTLFRLLAGLETPDTGTIEVTGRVVLVFQRPYL
                     FHGSVADNLLFPSRLRGMAHDEAIDSALRLARSLEIAHLLSHPVRHLSAGEAARASFG
                     RALAAQPDLLLIDETLVHLDAESLKPICATLEKFVASGGRFVLTTHQPSMALQLCHHH
                     LFLADGRLIEVQGADLQHDTPSVETPIVPKVM"
     gene            complement(3836..>4675)
                     /locus_tag="PKO06_05620"
     CDS             complement(3836..>4675)
                     /locus_tag="PKO06_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HNV69157.1"
                     /translation="TASSPSCCDMPAAPPENDSSISPASRIPAPPFAGLRHVEFPIAE
                     VLPFLDKASLFRSRWQYQKGQQSDAAYQTILETEAMPALEALLQRDRQEKIFRPQAAY
                     GYFPCRRSGNELIILTQSGAEAGRFAFPRRSRKPQLCLSDYFRSDGTDLIGLWAVTVG
                     SRASELAAEIYQQNHYRDYLHLHGLGIELAETTAELLFRQMQVELGFRPTESGPDLVK
                     AGLRYSFGYPACPDLSQQKLLLELVQAAKIGVSLTEAFVMVPEQSVSGIFIHHPEATY
                     FAP"
BASE COUNT         1051 a         1183 c         1455 g          986 t
ORIGIN      
        1 gagtcgcgaa gagagtgtac cggtgccatt ataatgcatt ccatgccgct tgacaccatc
       61 tggcaaaaaa gaataaagag acgaatagtg gtcaaaaagc tcaagggagg atgtacctca
      121 tggggatact cgtcggcaag gacgcaccgg atttcgttgc caaggcgatc atgggtgata
      181 acagcatcaa tgaacgcttc caactcaagt cgtttcttca ggggaagtat gggattcttt
      241 tcttttaccc actggatttc accttcgtgt gtccgtcaga gattctggcc ttcgatcatc
      301 gtctcgatga gtttcgcaag cggaacaccg agatcatcgg cgtgagtgtt gactcagagt
      361 tttctcatct ggcctggaaa aacactccgg tggaaaaagg cggcttgggt tgggtgcgat
      421 ttccgatggt gtcagacttg tcgaagtcga tcagtcgcga atacaacgtg ttaatcaatg
      481 attcggtggc tctgcgggcc acgtttctga tcgatcgaca gttgaaagtg cggcatcaac
      541 tggtgaacga tcttccactc ggacgcaatg tggatgaagc tttgcgtatg gttgatgcac
      601 tgcagtattt cgaaaagagc ggcgaggtct gtcctgccgg gtggaacaaa gggaagccgg
      661 ggatgaaagc aaccactgac ggcgtggctg aatatcttgc ttcgcacggg aaagacctct
      721 agggcgatct gagcggcggg tttcgcgtaa tcacgagcac gggggaagag cggttcctcg
      781 gacggaaagg gacggaaagg atcaggtggg ctcggcgcgg tgacttctca gcgttgcttt
      841 ccgtggcggg ctttctggtt gggatgaatg ccgagatacc ctttgctgat ccatctctcg
      901 gcaaggttgt cgtattcgtc tcgcaataag ctgaaatggt gcatgtcctg ccaggtgccg
      961 gccagaaaga gttcctggcg tatggttccg tcgtgggtga atccgaggcg tctgagtagt
     1021 tcctgtgtgc gcacgttgca atgcgtcgtt gctgcccaga tgcgatgcag cgggctttct
     1081 tcgaagacga acgaaagcat ggtggccagc gcttctttca tgaggccatt ccccgcagac
     1141 gattctgcaa gccagaatcc caggccacct gagagctgta ttccccacga gatgctgtgc
     1201 agcgagacct ctccgaccag tcgatgcgat tccttatcga agatggcgag atccagtcgg
     1261 gaaccctgac gctcgaggcg ttggcgtcgc aagatttcct gtttcatgtc agacagctca
     1321 aagcgggcgg gcaggggagg gagacccatc gccagccaac tctcgttgcg gacgagcagg
     1381 tcgagaagtg gctgagcatc gcgatgacga aaccggcgca agtagacgcg ggtggcgtac
     1441 aactcacggc tgaccggcaa caggtaggaa aggcccggac gcggcatgtc tctcatgcag
     1501 atgttttctt tctcccacag gtatcgtatc atgaaccatc agaaaggtct tattttttat
     1561 catgacactg aaagccgttt tcttcgatgt cggcggaact ctgatttttc cgaatccgga
     1621 acgaatcgga gcgacgtttg cccagacagg aagcgcgctt ccgaccgagc ggtggctcga
     1681 ggcggttcat cgtgcgtcag cctcactcga tgcgcaactg gaagcgggaa aacgaatggg
     1741 cgacgattgg tggcaggtgt attttggcct ggtcgtctcg catgcgactg gtcgaccggc
     1801 gcacgaatgg agcggtttca agaccttttt gaagcaactc aaggatgacc atgtcgctca
     1861 caacctctgg gacagcgttg ccccgggagc gcccgaaacc gtcgaacgtc ttcagatggc
     1921 gggattttct ctcggtgtga tttcgaacag tgacggcagg gttcgatatc agttgcaaca
     1981 agcgaagctg gcagagagat tttcgttcat tatcgactcg tacgaggtcg gctgcgacaa
     2041 accggatgca aagatctttc acctggggct gaagcatgcc ggattgaagc ctcagcaggt
     2101 gctctatgtg ggggactttg tcgagattga ccatcgtggg gcaacgggag tcggcatgga
     2161 ggccgtgatc attgaccccc tgcggttgag aagacacctc gacggagttc gttttatcga
     2221 ttcgatcagc cagttaccga gtcttctcga tctctgatca gacctggttc tgctggcaca
     2281 cgagatgcca cacacggctc acagcgaacg actcagtcga gcgaatagta ccggtcgaaa
     2341 atggtggcgt gagatctttc gcggaaggtc gttcgatcgt tgactacatg accttgggga
     2401 caatcggtgt ttcgaccgag ggggtgtcgt gctggagatc ggctccttga acttcgatca
     2461 agcgtccatc ggcgagaaag agatgatggt gacacagttg aagtgccatg ctcggctggt
     2521 gggtggtcaa gacaaacctg cccccagacg cgacaaattt ctcgagcgtg gcgcagatgg
     2581 gtttcaatga ttctgcatcc agatgcacca gtgtctcatc gatgagcagc agatcaggtt
     2641 gagctgccag tgcgcgtccg aacgaggccc gagcggcttc accggcagaa agatgccgca
     2701 ccgggtgtga aagcaggtgg gcaatttcca gcgaacgagc cagacgcagg gctgagtcga
     2761 tcgcttcgtc gtgagccatt ccgcgaaggc gcgagggaaa caacaggttg tcggcgactg
     2821 atccatgaaa aaggtagggt ctctgaaaca cgagaaccac gcgacccgtg acttcgatgg
     2881 ttcccgtgtc gggcgtttcg aggccggcca gtagtcggaa cagagtggtt ttgccacaac
     2941 cggatcggcc ctggagagag gtcccaccct cgagcggcaa ctcaaatgtc agattctgaa
     3001 agagtgggtt ggcaaatgac tttgagagtg cggtcagcct ggcggtgggc ggtgtgcgat
     3061 tcgccggaaa gagggatttg gtgtcagacg tcggagcact cgagactggc gtcaggtccc
     3121 ggtgcctgaa tttctcgacg aaatactgcg cgtgaccgat caggaaagcg tttgcgagcg
     3181 cggcccccag aagaatcaac ccgagggcga tcgagagttc cgtttccccc ttcccgatct
     3241 cgagcgaaat ggctgtggtc atggtgcggg aaacgccgcg aatgttaccg cccagaatca
     3301 tcgcggcacc cacttcggag acgacacggc caaaagcggt gcagacagcg atcaacagag
     3361 cttcgcgaca ctcattgaaa accacccgaa ccagttgcag gcgatgtgct cccagattgc
     3421 gggcggtatc aaagattcct ttcggcacga gactcagtgc ggaaatgaga aagagggcaa
     3481 ccaacggaat cgccagcagc aggtctccga ctatgatcgc ggtgggcgtg tacagcagct
     3541 cgagggcgcc cagcgggccg tttcgcgaca gcagcagaaa gcagacggtg ccgatgatga
     3601 cggttggaat cgcggccagg acttgaaaca gcagcgaaaa caggtatcgc cagcgtggtc
     3661 cggccaggtg aatgtgccag gcgatcggaa tcgccaccag cgttgcgccg acggtcgaga
     3721 cgacagcgat cttgagtgac agccagagaa tggcgagaac ttcagcgtct gcctgtatca
     3781 gcagccgcag agcacccgag aagctactga caatgaaggt cagcgaatcc atgcgtcacg
     3841 gggcaaaata ggtcgcctcc ggatggtgta tgaagatacc ggaaacagat tgctcgggca
     3901 ccatgacgaa ggcttctgtc agtgacactc cgatcttcgc cgcttgtacc agttcgagta
     3961 acagtttctg ttgagacaga tcgggacacg caggataccc aaacgaataa cgcagccccg
     4021 ctttcaccag atccggtcct gactctgttg gtcgaaatcc gagttccacc tgcatttggc
     4081 gaaacagcag ttcagcggtg gtttcggcca gttcgatgcc gagtccgtgc aggtgcaggt
     4141 aatcgcgata gtgattctgc tgatagattt ctgctgccag ttctgaggcg cgggaaccga
     4201 cggttaccgc ccagagtccg atgagatctg tgccgtctga tcgaaagtag tcggaaaggc
     4261 agagttgcgg cttgcgagat cggcgcggaa acgcgaacct gcctgcttct gcgccgcttt
     4321 gggtcaggat gatcagttcg tttcctgacc ggcgacacgg gaagtatccg tatgctgcct
     4381 ggggtcggaa aatcttttcc tgtcggtcgc gctgaagcaa tgcctcgagt gcgggcatgg
     4441 cttcggtttc cagaattgtc tggtaggcgg catcgctttg ctgccccttc tgatactgcc
     4501 agcgcgagcg gaacagactg gctttatcaa ggaaggggag gacctcggct attggaaact
     4561 cgacatggcg aagacccgca aacggtggcg ctggtattcg agatgccggc gagatggaac
     4621 tgtcattctc gggcggggcg gctggcatgt cgcagcagga gggtgacgag gccgt
//
LOCUS       DAORHD010000201         4664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19422_length_4664_cov_3.430245, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000201 DAORHD010000000
VERSION     DAORHD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19422_length_4664_cov_3.430245"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="PKO06_05625"
     CDS             <1..632
                     /locus_tag="PKO06_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNV69158.1"
                     /translation="LLRAGGNIVSLHLSSKMSGTWQSAMLAKKELGSDRIQVVDALST
                     SIGLGNLVAVCARAAHAGRSAADILGLATHMIATHRLLFVVDTLEYLRKGGRIGLAAA
                     FIGGLLNIKPILSLRDGQVCLLERCRGSKNVQNRFIELVNEYLVQHSGKKICTAVTYA
                     GDSANLDAMLRAMSQHLDTSHALRTEIGAVVGAHVGPGCYGISLHCLPD"
     gene            1152..2024
                     /locus_tag="PKO06_05630"
     CDS             1152..2024
                     /locus_tag="PKO06_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69159.1"
                     /translation="MRQHTQLPGSFRILLDRAERDDTLLRLWYSGREENSRNSLQPLA
                     VYEFDLRLQRVVRRDLTPLAHSFPALSGQFLDGTSVSTRCLALKAEYDRGGLTIRGVL
                     RNLPGYILETPNFTSRFRIWAEANGAVVATDAQLLGTTVYELSGVPVTAGRVTIKAYI
                     RGYDLVTLHPGVTLNQSQPIGTIAGIDIDFHQMRPITRDVRLLIRSNGGDEDASFPPG
                     ARARVYCKETGHAAETVSQNFFGEAWLMDVPTGWPLNFQAINLDSGWFSGSLGPITIP
                     ENGGTAFTESITLE"
     gene            complement(2029..2379)
                     /locus_tag="PKO06_05635"
     CDS             complement(2029..2379)
                     /locus_tag="PKO06_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69160.1"
                     /translation="MAGEESTARFRIESVQLEEKNECDGWFVETLYEWRIIDTVTGTV
                     VRRIPYRHVVDNTEWPERDFWNGPRRVEIVGDEVRAYLTVSDRTGVSFEGQPDESAGY
                     DIYPLPTASQTRSP"
     gene            2530..2979
                     /locus_tag="PKO06_05640"
     CDS             2530..2979
                     /locus_tag="PKO06_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69161.1"
                     /translation="MAETGQRSPSLSEFFLADGLLTEDQIQTIWKQADQTGVGFGTAA
                     VKLGLLDELQVAQALGELCHVPYLDLDNYLVNPVIARLIPEHISRRHRLIAINRIVNR
                     LTIAMTDPVNIIAIDDIQLMTGLQVKVVVAQESAIVKTLDQCFSEVG"
     gene            complement(3037..4095)
                     /locus_tag="PKO06_05645"
     CDS             complement(3037..4095)
                     /locus_tag="PKO06_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008046227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HNV69162.1"
                     /translation="MHFGSDNQTGASEKVLQKVLEANRGVTAGYGDDLWSQRAVEALR
                     QMFACDLDAHFVPTGTAANGLALGSLVQPFESILCHNQAHLLTSEGSAPEYLTGGARL
                     IGISRGEAKLRPEHLDQHFRQMGHGHDHNTPPKALSVAQVAENGLVYSPAELKALCES
                     AHRFGLRVHLDGARFANAVAALGCHPGDISWRAGVDVLSLGATKNGALAAEAVVFFQR
                     GSGDDFQRRRKRAGHTVSKGRLFGAQFLGWLEENHWLELASHANQMACKLGQALQSIP
                     DIRLVFPVMANELLLIMPRSMADRLAAAGARFFEWYVESLPAEIRLREDEAYIRLVTS
                     FCTTAAEIEEFRQVAAGA"
     gene            complement(4096..>4664)
                     /locus_tag="PKO06_05650"
     CDS             complement(4096..>4664)
                     /locus_tag="PKO06_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69163.1"
                     /translation="AEYLDWWEYSGRPSIQVYAAGGERKWFSVLHLHGKGTGIYDVRH
                     HFYRLIDDQVVTTLVLPAEVRYFWIGMPLHTEVSAIFVPNEPESLSVRFRCRCYAFEE
                     WSDGSPFFDNEIDVWYKWNEPERRLVPEIPQEDNTKAKLDCLYSGSVMPAQFLKAFAA
                     EAAELEQHGSLHQRRTVKRLRQWREKEE"
BASE COUNT          950 a         1431 c         1291 g          992 t
ORIGIN      
        1 agctgttgcg cgcaggcgga aacatcgtct cgctgcatct gtcctcgaag atgagcggca
       61 catggcagtc agccatgctg gcgaagaaag aacttgggtc ggaccggatc caggttgtcg
      121 atgcgctttc cacctcgatc ggtttgggca atctcgtcgc cgtctgtgct cgagcggctc
      181 acgcgggtcg ttcggcagcc gacattctgg gactcgccac ccacatgatc gccacgcacc
      241 gtctgctgtt tgtcgtcgat acgctcgagt atctgcgcaa gggcgggcga atcgggctgg
      301 cggcggcgtt catcggcggt ctgctgaaca tcaagccgat tctcagtctg cgtgacgggc
      361 aggtctgcct gctcgaacgc tgccggggtt cgaagaacgt gcagaaccgt ttcatcgagc
      421 tggtcaacga gtatctcgtc cagcattcgg ggaagaagat ctgcaccgcg gtgacctatg
      481 ccggtgattc ggccaacctc gacgccatgc tgcgcgccat gagccagcat ctcgatacgt
      541 cgcacgcctt gcgcaccgaa atcggtgcgg tggtcggcgc ccatgtcggg ccgggctgct
      601 acggcatctc gctgcactgc ctgcccgact gaccagagaa ccacgttccc gagcaggcac
      661 ccggggaaat gcacactttt cctgcgtggg ctcgggcttt tcgactcacc cgcgtcagac
      721 cgctccatgc ccactgagtg ccggccaatc gcttcgcggg atgacaccga tttccctcga
      781 gagccgactc ctgtcggcgc tctcggcgac gtccatcatg gacgtcgaac ggtgtcatcg
      841 actctcgctc ttggggcact caggtgcatt ctgcttgtct tccttcgggt gagtcgaaaa
      901 gccctatgga tcaagcgaaa gtttgcattt gccttggagc aggcacccga gccggttgcg
      961 agaaccgccg actccgctta gaataagaga aattgctgtg ctgcgaggtg tctgcgatgg
     1021 ttcctttcag aattaggggg cgtgttgtgg ccggggtcgg cgtgttcgtt cttctgttgg
     1081 cctctctctc gctcgcaacc gaactgtcga caatagcaac agtgcgggtg gcagacgccc
     1141 tccgggagga cttgcgccag catacccagc ttccgggttc gttcagaatt cttctcgatc
     1201 gcgccgaacg cgatgacacc ctgctgcgac tctggtattc cggtcgagag gagaactcgc
     1261 ggaactcgct tcagccgttg gccgtgtatg agtttgatct gcgcctgcag agggtcgtgc
     1321 ggcgtgacct gacaccactt gcccactcat ttcctgcgct gagcggccaa ttcctagacg
     1381 gtaccagtgt gtcgacacgt tgtctcgcat tgaaagccga gtatgatcga ggtggattaa
     1441 ccattcgggg cgtgttacgc aatctgcccg gttacattct cgaaacgccg aacttcacgt
     1501 cacggttccg catctgggcc gaggccaatg gggcggttgt cgcaactgat gcgcagctgc
     1561 tgggcaccac cgtgtatgag ttgtctggag taccggttac tgccggccgc gtgacgatca
     1621 aagcttacat tcgcgggtat gatctggtca ccctgcaccc cggggtgacg ctcaatcagt
     1681 ctcagccgat cggcaccatc gccggcatcg acatcgactt tcaccagatg cgaccgatca
     1741 cgcgcgatgt tcgcctgctc attcgcagca acggtggcga cgaggatgcg tcattccccc
     1801 cgggggcccg ggcgcgcgtc tactgcaaag aaaccgggca tgcggctgaa accgtttcgc
     1861 agaacttttt cggcgaagcc tggctgatgg atgtccctac cggctggccc ctgaattttc
     1921 aggccatcaa tctggactcc ggctggttct ccggcagcct tggcccgatc acgattcccg
     1981 agaatggcgg cacagctttc accgaaagca tcacgctcga atagctcctc agggggaccg
     2041 ggtctgcgac gccgttggca gggggtagat gtcgtagccg gcagactcat ctggctgccc
     2101 ttcgaagctg acaccggtgc ggtcggaaac cgtgagatag gctcgcacct catcgccaac
     2161 gatctccaca cgtcttggcc cgttccagaa atctcgctcg ggccattccg tattatccac
     2221 cacgtgccgg tacggaatcc gtcgcacgac agtaccagtc accgtatcga tgattcgcca
     2281 ttcatacaag gtttcaacaa accaaccatc gcactcattc ttttcttcaa gctggacact
     2341 ttcgatgcga aagcgcgctg tcgattcttc ccccgccatg cccgaacctc cggcttgtca
     2401 gtttcgaaac attgtagccc aaaaacgttc caaaatgcac atcgtccgtt gcctttcgag
     2461 cgaaatctcg tagcagaatg aaaaaacgtc tgctatcgtg gaggaacagc acatcaagag
     2521 gagaaggcta tggccgagac agggcaacgg tccccttcac tttcagaatt ttttctcgcg
     2581 gatggtcttc tcactgagga tcagattcag accatctgga agcaggctga tcagaccgga
     2641 gtcggattcg ggactgcagc ggtcaaactg ggtctgctcg atgaactgca agtcgctcaa
     2701 gcgcttggcg agttatgcca tgtcccatac ctcgatctgg acaactatct cgtcaacccg
     2761 gtgatcgccc gactgattcc cgagcacatc tcgcggcggc accgtttgat cgccatcaat
     2821 cgcatcgtaa accggctgac catcgccatg acagaccccg tgaatatcat cgccatcgac
     2881 gacattcaac tgatgacagg cctgcaggtg aaggtggtcg tggctcagga gagcgcgatc
     2941 gtcaagactc tcgaccagtg tttttccgag gtcggttgag cactttccgt ttcttgttct
     3001 caagacggca ctgaaaatca gaacatggtc gcggtgtcag gcgccggcgg ccacctgacg
     3061 gaattcctcg atctccgcgg cggtcgtgca gaatgaggtt accagtctga tgtaagcttc
     3121 atcttcgcgc agacggattt cggccggcag cgactcgaca taccactcga aaaacctggc
     3181 tccggcggcc gccagtcggt cagccatgct acgcggcatg atcagcagga gttcattcgc
     3241 catgaccgga aacaccagcc ggatatcggg gatcgactgt agtgcctgtc ccagtttaca
     3301 tgccatctga ttcgcatgcg atgccagttc cagccaatga ttttcttcga gccagccgag
     3361 gaattgcgcg ccaaacagtc tccccttgga aacggtatgc ccggctcgct tgcgccgtcg
     3421 ctgaaaatcg tcaccagaac cgcgctgaaa gaaaacaacc gcttcggccg ccagcgcgcc
     3481 gttcttcgtc gccccgagcg agagcacgtc gacacccgcc cgccagctga tgtcacccgg
     3541 gtgacagccc agagccgcga cggcattcgc gaagcgggcg ccgtcgagat gcacccgcag
     3601 gccgaatcga tgcgccgatt cgcacaacgc tttcagttca gccggagagt agaccaggcc
     3661 gttttcggcg acctgcgcga cactcagagc cttgggtggc gtgttgtgat catgtccatg
     3721 ccccatctgg cggaaatgct gatcgaggtg ctctggtcgc agctttgcct caccgcgtga
     3781 gataccaatc aatcgtgcgc ctccggtaag atactccgga gcactgccct ctgacgtcag
     3841 cagatgcgcc tggttgtgac acaagatgga ctcaaaaggc tgtaccagtg aaccaagcgc
     3901 cagcccgttc gccgccgttc ccgtcggcac aaaatgcgcg tcgaggtcac aggcaaacat
     3961 ctgtcgcaac gcttccacag cacgctgtga ccagaggtca tcaccgtagc ccgccgtgac
     4021 gccacgattg gcttcaagca ctttctgcag caccttctct gatgcccccg tctggttgtc
     4081 gctgccaaaa tgcattcatt cctccttttc acgccactgt cgcaggcgtt tgacggtacg
     4141 ccgctggtgc agggaaccat gctgttcgag ttcagcggct tctgccgcga atgccttgag
     4201 aaactgggca ggcatcacag aaccggaata cagacaatca agtttcgcct tcgtattgtc
     4261 ttcctgcgga atctctggaa caagtcggcg ttccggctca ttccatttgt accagacgtc
     4321 gatctcatta tcgaagaatg gagaaccatc agaccactct tcgaatgcat agcaacggca
     4381 ccgaaatctc accgacaggc tctccggctc gttcggaacg aagatcgccg agacctccgt
     4441 gtgcagcggc atgccgatcc agaaataccg cacttctgcc ggcagaacga gcgtcgtcac
     4501 gacctgatca tcgatcaggc gatagaaatg atgacgaaca tcgtagattc ccgtaccctt
     4561 tccatgcaga tgcagcacag aaaaccattt gcgttcaccg ccggcagcgt atacctggat
     4621 actcggacgt cccgagtatt cccaccagtc aagatattca gcat
//
LOCUS       DAORHD010000202         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19461_length_4656_cov_3.958053, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000202 DAORHD010000000
VERSION     DAORHD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19461_length_4656_cov_3.958053"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="PKO06_05655"
     CDS             <1..1500
                     /locus_tag="PKO06_05655"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69164.1"
                     /translation="LQVLRLTAKLSCDIERHERLSKRISNLPEPKTDNAFIDALTRDF
                     RGRRGKTRTLSVGAKAQVIYTHFLYDAQELPIGIVFMIHQNRYLEHEYLRSMLKNPPP
                     WCQAPFPIKVFSVSENGAMHSMISRNLGSAVIDELSDSVTQTQAPQHRFISIPGDSER
                     YLLTAIPSRYLSALRLYMISSLEQIEQRSRRLVQAFTLFGVCITLLGLSLSLVFSNLL
                     LHPMARIGEGLQQLAAGKFSHRINVCTGDEMEELSSGLNKTLEEMQELAVAKNIQEHL
                     LPAAPLQTPSGSCQGWIRSQSEIGNEIYDFQSLSQGRIGFWLAGVPGHTISSALILAM
                     MKMAGRLFLSFGESSPEHILDQFVRFIRDKVQTNGDIYLCIGIYDPQQQQTRLSMQGS
                     FFTACVSRPMNHPAAVRLTSEGAPSSSERRTLTVPLDRDERLLLLSPGFLARTTFHHF
                     EMFLQSQIREHGAGSLQQLGDSLLANTTEELSDAEPTTRSLVALEVSGT"
     gene            1497..4547
                     /locus_tag="PKO06_05660"
     CDS             1497..4547
                     /locus_tag="PKO06_05660"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69165.1"
                     /translation="MTTFFSWRRAPFFLAFWVVPSILYWVTIALVGLVIERHETEVLQ
                     NQVSERIFSICRGYEEGIKPFFNDVFQKYRRTSFNTTEFEKTHQQVMNRFGNRRTCIY
                     LFDVQGRPLPLPGQQIPPGLERLFTFIRTPWSQSFPVDDSLWKPLDQILFIPSFVSRL
                     RERENTFVMLGHEDKFERHRMFGMYRWDPDTPAGVPAGMLAFAWIFTFDMQFRLEHQL
                     QTHPQDHLSSSFYSNHGHHLTASAVSPEFLKRVSEQYQISGQEHLIFEGQIIHCIAFD
                     ELGLLITSTPLPSLPLWMKATAILIYLLLSRLSLRFSIRLLSTDSESPNPIESEPTQR
                     RIDLATKLLLLFCIGALVPLISTGTLGILYLSEKRQELIQTNQTMAINTLDQIDREFE
                     GSCQQRQLFYEKLAQGFGRGSETYDTVLHTLETLRNNSQIDDIFIVSSQSQLLVVQHA
                     RQRNGYRWLVSRPLPERQEIFARLLTSPTFKFTYDEALCLIGKADNDEITSRITHKHT
                     EKLKKLLPKVARECIDYSNEIEGIKTLKSSVNSDLLVSEMAGSDAETSFRFLKLSQGK
                     LIPVPRAIGEGFLYVVTLKDTRREAQYVVFLFHDYASLHRDYLLQACANASRPDTGIL
                     VRAYNFEDPVCIAYPIMAHDPYEVVRDMLNRARRDSLYLHVGEGTEERLITARVPKQL
                     SDISLVHVLPATVIEQQLSPYRLRVFGCMAGVTLICLTLGWILMQRFLVPVYQLAEGV
                     KAISNRDFSHRVVIQSRDELGLLGHTLNRTIAYLSEMEMALTIQRTLFPTGNLTVGTV
                     EISGRNEMTQAVGGDYFDYFTLAGDRAVVLLGDVSGHGVSAALVTAMAKAGFHLLFER
                     YPADPRQVFEQMNLQMYEILRRKKMMTCLCGLIDLKNQTMQMLNAGQSFPVMVADNGS
                     PQLCELVSNPLGVLKKGKFAESTIPLTGIGIVLYSDGLIEALNADGQMFTYDRFQQAA
                     ATALNQLQATPAQTIFQTVRQFTGSIPWNDDATVVIIRPIKPHP"
BASE COUNT         1005 a         1389 c         1166 g         1096 t
ORIGIN      
        1 ctgcaggtgc tgcggctcac cgccaagctc agctgcgata tcgagcgcca tgagcggctc
       61 agcaagagaa tctccaatct gccggagccc aagacggata acgctttcat cgatgcgctt
      121 acccgtgatt tccgggggcg gcgaggcaaa acccgaactc tttcggtggg tgccaaagcg
      181 caggtcattt atacgcactt tctgtatgac gctcaggagc tgccgatcgg aatcgttttc
      241 atgattcatc agaatcggta tctggaacat gagtatctgc gctcgatgtt gaaaaatccc
      301 cccccgtggt gccaggctcc atttcccatc aaggtcttct ccgtgtccga aaacggagcc
      361 atgcattcca tgatcagccg caatctcggt tcagccgtca tcgacgaact cagtgattct
      421 gtcactcaga cacaagctcc tcagcatcgt ttcatctcaa ttccaggcga ctccgagcgg
      481 tatctgctca cggcgattcc ttcacggtat ctgtctgcac ttcgtctcta catgatcagc
      541 tcgctggaac agattgaaca gcggtcgcgc agacttgtcc aggcatttac tctgttcggg
      601 gtttgtatca ccttgctggg gctgtcgctc agtctcgtct tctcgaacct gctcctgcac
      661 cccatggccc gcatcggcga ggggttgcag cagctggccg ccggcaaatt ttcacaccga
      721 atcaacgtgt gtaccggtga tgaaatggaa gaactgagtt caggactgaa taagacactt
      781 gaggaaatgc aggaactggc tgtggctaag aacattcagg aacatctgct gccggccgct
      841 ccactgcaga cgccgtcggg atcttgtcag gggtggattc gcagccaatc ggaaatcggg
      901 aacgagatct acgactttca gtcgctctct caaggaagaa tcggcttctg gcttgccgga
      961 gttcccggac acaccatcag ttcggcactg attctggcca tgatgaaaat ggcgggccgc
     1021 ctgtttctca gctttggtga gtcttcaccc gagcatatcc tcgaccagtt tgtccgtttc
     1081 atccgcgaca aagtgcagac caacggcgac atctacctct gcatcggtat ctatgatcca
     1141 cagcaacagc agacacgact cagcatgcaa ggctcctttt tcacggcctg tgtgtcgcgt
     1201 ccgatgaatc accctgccgc ggttcgactc acgagcgagg gtgccccttc atcgtctgaa
     1261 cgccgaaccc tcaccgttcc cctggaccgc gacgaacggc tgctgctgct ctcgccggga
     1321 ttcctcgctc gcaccacgtt tcaccatttc gaaatgtttc tgcagtctca gattcgcgag
     1381 catggcgcag gctctttaca gcagctgggc gactctctgc tggcgaacac caccgaagaa
     1441 ctttcagatg cggaaccaac gaccagaagc cttgttgccc tcgaggtctc tggtacatga
     1501 caaccttctt cagttggcgt cgcgctccgt tctttctcgc attctgggtt gttccatcga
     1561 ttttgtactg ggtcaccatc gccctcgtcg gactggtgat cgagcgtcac gagacggaag
     1621 tgctgcagaa ccaggtctca gagcgtatct tctcgatttg tcgcgggtat gaagagggca
     1681 tcaagccgtt cttcaacgac gtttttcaga agtatcgccg cacgagtttc aacacgaccg
     1741 agtttgagaa aacgcatcag caggtcatga accggttcgg aaatcgccgc acctgcatct
     1801 atctgttcga tgttcagggc cgtccgcttc cccttcccgg acagcagatt ccgcctggtc
     1861 tcgaacgact gtttacattc attcgcaccc cttggtcaca gagcttcccc gtcgacgact
     1921 cgctctggaa gccactcgat cagatcctgt tcatcccgag tttcgtttct cgtctgcgtg
     1981 aacgcgagaa cacctttgtc atgctcggcc atgaagacaa gtttgaaaga catcgcatgt
     2041 tcggcatgta tcgctgggat cccgatactc ctgccggagt tcctgcagga atgctggctt
     2101 ttgcctggat cttcacattt gacatgcagt ttcggctcga gcatcagctg cagacacacc
     2161 ctcaggatca cctctcgagt tctttctact ccaaccacgg gcaccatctg acggcttccg
     2221 cagtctcgcc ggagtttctg aaacgtgtgt cagagcagta tcagatttca ggccaggaac
     2281 acctgatctt cgaggggcag atcattcact gtattgcctt cgatgaactt ggtctgctca
     2341 tcaccagcac acctctgccc tcacttccgc tctggatgaa agcaacagcc atcctgatct
     2401 atctgctgct gagtcgtctg agtcttcggt tttccattcg tctgctttcg accgactcgg
     2461 aatcaccgaa cccgatcgag tcggaaccca cgcagcgtcg catcgatctg gcaaccaaac
     2521 ttctgcttct tttctgcatt ggagctcttg ttccactcat ttcgactggg actctcggaa
     2581 ttctgtacct gtcagaaaag aggcaagaac tgatacagac taatcagaca atggccatta
     2641 acactctcga tcagatcgac cgggaattcg aaggaagctg ccagcagcgc cagctttttt
     2701 acgagaagct ggctcaggga ttcggtcgcg ggagcgaaac gtatgacacc gtgctgcata
     2761 cccttgaaac gcttcgcaac aacagccaga ttgacgacat cttcatcgta tcgtcgcagt
     2821 cgcaactcct cgtggtccag catgctcggc agcggaacgg atatcgctgg ctggtttccc
     2881 gcccgctccc cgaacggcag gaaatcttcg cgaggcttct cacgagtcca acgttcaagt
     2941 tcacgtatga tgaagcactc tgtctgattg gcaaggcgga taatgacgag atcacctcac
     3001 gcatcaccca caagcacacg gaaaaactga aaaagctgct gccgaaggtc gcacgggagt
     3061 gtatcgacta ctccaacgaa atcgaaggga tcaaaacact caaaagcagt gtcaattccg
     3121 atcttctggt ctcagagatg gccggcagcg atgcggaaac ctcttttcgt tttctcaaac
     3181 tgtcgcaagg caaactcatt cctgttccgc gcgccattgg cgaaggtttt ctctacgtgg
     3241 tgacgttgaa agacacacgg cgtgaagctc aatacgtcgt ctttctgttt cacgattacg
     3301 ccagcctgca tcgcgactac ctgcttcagg cgtgcgccaa cgccagccgc cccgataccg
     3361 gaattctggt tcgcgcctat aactttgagg accctgtctg catcgcgtat cccattatgg
     3421 cgcatgaccc ctacgaggtc gtacgcgaca tgctgaatcg cgctcgtcgc gactcgctgt
     3481 acctacacgt gggtgaagga accgaggaac gcttgattac ggcgcgagtg ccgaaacaac
     3541 tgtccgatat ctccctggtt cacgttcttc cggcgacagt catcgagcag cagttgagtc
     3601 cctaccgact tcgcgtcttt ggctgcatgg ccggtgtcac cctgatctgt ctgacactcg
     3661 gctggattct gatgcaacga tttctcgtgc cggtgtatca gctcgcagag ggtgtcaagg
     3721 caatcagtaa ccgggacttt tcgcatcgcg ttgtcattca atcacgcgat gaactgggtc
     3781 tgctcggaca cacgcttaat cgcaccatcg cctacctcag tgagatggag atggcgttaa
     3841 ccattcagcg cacgctgttt cctaccggaa atctgactgt cggcactgtg gaaatcagcg
     3901 gacgcaacga gatgacacag gcggtcggcg gcgactactt cgactacttc actctggcag
     3961 gtgaccgggc tgttgtcctg ctgggtgacg tctccggtca cggagtctcg gccgcacttg
     4021 tgacggccat ggcgaaagcc ggttttcatc tgctgtttga acggtatccc gccgacccgc
     4081 gccaggtgtt cgaacagatg aacctgcaaa tgtacgagat attacgccgc aagaagatga
     4141 tgacctgcct gtgtggactg attgatctca aaaatcaaac catgcagatg ctcaatgccg
     4201 ggcagtcttt tcccgtcatg gttgccgaca acggctcacc ccagctctgc gaactcgtct
     4261 ctaacccgct gggcgtgttg aaaaagggca aattcgccga atcgaccatt ccgcttaccg
     4321 gaatcggtat cgtcctctat tcagatggtc tcatcgaagc actcaacgcc gacggccaga
     4381 tgttcaccta cgatcgtttc cagcaggcgg cggccactgc gctgaatcaa ctccaggcaa
     4441 ctcctgccca gacgattttc cagacggtca gacagttcac cggcagcatc ccctggaacg
     4501 acgacgcgac ggtggtcatc attcgcccca tcaaacctca cccgtgaact tcatcacacc
     4561 tgctgtcaca gacttcagac gtgtggcaca atcctgcgga acctccgggc tggcttgtga
     4621 tcgttccaag ttgccctacc tctcatactc ccgaga
//
LOCUS       DAORHD010000203         4639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19581_length_4639_cov_4.886998, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000203 DAORHD010000000
VERSION     DAORHD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19581_length_4639_cov_4.886998"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PKO06_05665"
     CDS             <1..460
                     /locus_tag="PKO06_05665"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNV69166.1"
                     /translation="ELLRLWGLRYIGPTTRTREICADRLVTGGPYARVRHPLYLANAL
                     KILGLLIIAGHLPLALTAIAFYAVEFFNMIPYEEHFLAQRFPKEFEAYRSSVPAFLPD
                     GSLFASDFQATHTWTEALNSERRTFASTGGLLGILAFCTWLRGSPAEADR"
     gene            457..1611
                     /gene="lpxB"
                     /locus_tag="PKO06_05670"
     CDS             457..1611
                     /gene="lpxB"
                     /locus_tag="PKO06_05670"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV69167.1"
                     /translation="MTNPKRLFFVAGEHSGDLHGSLIISHLKRFDPTLRIEGIGGPLM
                     QSVGCENLHCIDELAVIGFIEVLKNFRRLYDIFNDVRRHLEETRPDLLILIDYPGFNV
                     RLAEAAKAMGIRVLYYICPQVWAWHASRVNKITRVINEAVVVFAFEEAIWKQAGATVS
                     WHGHPLIDIVKASTDRQTFRAELSLGDGPVVSLLPGSREQEIHYILPLLLDTAALILK
                     DRPSSRFLLPLAGTIDDGLIQPHLRNRSLPLQILRGRTYDAVASSDLALVASGTATLE
                     TAILGTPLIVVYQTNLFTSVLSKFLVQSPHIGLPNVVAGRQVVPEFIRWNFTPANVAR
                     EALSLLANPDRLQKMRAELGDVRRTLGQPGAGERVAEHIFRGLQKQGGPT"
     gene            1608..3359
                     /locus_tag="PKO06_05675"
     CDS             1608..3359
                     /locus_tag="PKO06_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69168.1"
                     /translation="MTTFLRVLRYFRPYAPHLAAALVFTLIVAVCTIYPAWIIRSVVN
                     DVLVRGDMAMLHFIAISLVVVMAVKGVANFIQTYIITWAAQKILLTLRSQCFDRLLHL
                     SLSYYEKARTGELMSRILNDVAVLQHLLSQTTGIIGDFTAFLGFCGYIFYLHWKLALI
                     SIVIIPFIGALINSLSRTMKKIGTRMQSRIGDIATVLQEHITGVKVVKSFTLEEFERG
                     RFEKANLETFRENMKGNLINSATSPVIEFINTVGLAIIFWYGGYEVIQKRLDAGELIS
                     FLTALVGLFTPIKNISKFGNIVSQSVGGAERVFQVIDAPIEITEPAQPKTLEKWQGAV
                     AFEQVHFAYKPNEPILSGLSFEVKPGEVIALVGPSGAGKSTFVNLIPRFFDVQAGRIT
                     IDGIDVRDFRLSDLRGHIGIVPQETLLFSGTIAENIRLGRLDASQTEIEEAARLSNAH
                     DFIHSFPEGYNTYLGERGVNLSGGQAQRIALARAFLKDPPILILDEATSALDSESENL
                     VKDSLNKLMRNRTTFLIAHRLSTIINADKIVVLQNGGIVECGPHKQLLADQGLYSQLY
                     HAQYAHETMPQGRMHAH"
     gene            3349..4041
                     /locus_tag="PKO06_05680"
     CDS             3349..4041
                     /locus_tag="PKO06_05680"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV69169.1"
                     /translation="MPTDLTHESDLEARWDEPGYAQKARIKGRCAGLFVKLWASSLRV
                     EHIGADRAPLLHAAGRRVLFVVWHGSQLVPLATYRNRGISIMTSLSRDGDIQDSCMKT
                     LGFTTVRGSSSRGGARALLAMVRSLEKTGLAAIAVDGPRGPYHQAKPGAVVLAQKTGA
                     IVLPVGAAHTRCRHLRSWDKFELPLPFSRSVVWTGEPFEMPARCSPEEGCRLIEEHLQ
                     RCEDQARERIAA"
     gene            4038..>4639
                     /locus_tag="PKO06_05685"
     CDS             4038..>4639
                     /locus_tag="PKO06_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV69170.1"
                     /translation="MTQSTERKRILIVAGETSGDMYAAALVDELERLGEFEIFAVGGP
                     QTAKRKVNLLYDSSDWAVIGYVEAIKRAPQLYLVLRDLKRFLEQRRPHLVLLLDYPGF
                     NMRLAKHAKSLGIPTLHYFPPSKFATTPEEVADAASTLTCVAANFTFTYENYKAANAN
                     VHFVGHPLVDLAKPTMSREEAFRTFGLDPARPVVGLCPGSR"
BASE COUNT          868 a         1456 c         1354 g          961 t
ORIGIN      
        1 cgaactgctg cggctctggg gtctgcggta tatcgggccg acgacgcgca cgcgcgagat
       61 ctgcgccgac cgtctggtca ccggcgggcc gtatgcccgc gtgcgacacc ccctgtatct
      121 ggccaacgcc ctgaaaattc tcggactgct gatcatcgcc ggtcatctgc ccctggccct
      181 gacagcgatc gcgttctatg ccgtcgagtt cttcaacatg attccgtacg aggagcattt
      241 tctcgctcaa cgctttccaa aggaattcga agcctatcgt tcctcggttc cggcgtttct
      301 tcccgacgga tctctttttg ccagcgactt tcaggcaaca cacacctgga cagaagccct
      361 gaattcggaa cgccgcacct tcgcctcgac gggcggactg ctgggcattc tggccttctg
      421 cacctggctg cgcggctcac ccgcggaggc tgatcgatga cgaacccgaa gcgtttgttt
      481 ttcgtcgccg gtgagcacag cggtgacctg cacggttcac tgatcatttc gcatctgaag
      541 cgctttgatc cgaccctgcg cattgagggc atcggcggtc ccctgatgca gtcggtcggc
      601 tgcgagaatc tgcactgcat cgatgaactg gccgtgatcg gcttcatcga ggtgctgaaa
      661 aactttcgcc gtttgtacga cattttcaat gatgtccgtc gacatctcga ggaaacacgc
      721 cccgacctgc tgattctgat cgattatccc gggttcaacg tacgtctcgc cgaagcggcc
      781 aaagccatgg gcattcgggt gctctactac atctgcccgc aggtgtgggc ctggcatgcc
      841 agccgcgtca acaaaatcac acgagtcatc aacgaagccg tcgtggtttt tgcctttgaa
      901 gaagcgatct ggaagcaggc cggtgccacg gtttcctggc acggtcaccc gctgatcgac
      961 atcgtgaagg caagcaccga ccgtcagacg ttccgtgccg aactgtctct gggcgacggt
     1021 ccggtggtca gcctgctgcc gggcagccgc gaacaggaga ttcactacat tctcccgctc
     1081 ttgctcgaca ccgcggcact gattctgaaa gatcgcccca gcagccgttt tcttctgcca
     1141 ctcgctggga caatcgacga tgggttgata cagccgcatc tgcgcaaccg ttcgctgccg
     1201 ctgcagattc tgcgcggtcg cacgtatgac gcggttgcca gttccgatct ggccctggtc
     1261 gcctccggaa cggccacgct cgaaacggcg attctcggca cgccgctgat cgtggtgtat
     1321 cagaccaacc tgttcacctc ggtgctgtcg aagttcctgg tccagtcgcc gcatatcggg
     1381 cttcccaacg tggtcgccgg ccggcaggtc gttcccgagt tcattcgttg gaacttcacc
     1441 cccgccaacg tggcccgcga ggcactttcg ctgcttgcca acccggaccg tctgcagaag
     1501 atgcgcgccg agcttggcga cgtgcgcagg accctggggc aaccgggcgc gggtgaacgc
     1561 gtggcggaac acatttttcg cggtctgcag aagcagggag ggccgacgtg acgaccttcc
     1621 tgcgcgtgct gcgatatttc agaccctatg cgccacatct ggccgcggcg ctggtgttca
     1681 cgctgatcgt cgcggtctgc accatctatc cggcctggat catccgttca gtggtcaacg
     1741 atgtgctggt gcgcggcgat atggccatgc tgcacttcat cgcgatttct ctggtcgtgg
     1801 tgatggcggt gaaaggcgtc gcgaacttca ttcagaccta catcatcacg tgggcggccc
     1861 agaagattct gctgacgctg cgctcacagt gcttcgaccg actgctgcat ctctcgctct
     1921 cgtattacga gaaagcccgc accggcgaac tgatgagccg cattctcaac gatgtcgccg
     1981 ttctgcagca tctgctgtca cagacaacgg gcatcatcgg cgacttcacc gcgtttctcg
     2041 gtttctgcgg ctatatcttc tacctgcact ggaagctggc gttgatctcg atcgtcatca
     2101 ttcctttcat cggggcgctg atcaattcgc tgtcgcgcac catgaaaaag atcggcaccc
     2161 gcatgcaaag tcgcatcggc gacatcgcga ccgtgctgca ggagcacatc accggggtca
     2221 aagtggtgaa gtccttcacc ctggaagaat tcgagcgcgg ccgcttcgaa aaagcgaacc
     2281 tcgagacttt tcgcgagaac atgaaaggga acctgatcaa ctcggcgacc tcgccggtga
     2341 tcgagttcat caacacggtc ggtctggcga tcatcttctg gtacggcggg tatgaagtca
     2401 ttcaaaagcg tctcgatgct ggtgaactga tcagctttct gactgccctt gtcgggttgt
     2461 tcacgccgat caagaacatc tcgaagttcg gcaacatcgt ctcgcagtcg gtcggtggcg
     2521 ccgaacgcgt gttccaggtg atcgacgccc cgatcgagat caccgaaccc gcgcagccga
     2581 aaaccctcga gaaatggcag ggagcggtcg ctttcgagca ggtgcatttc gcctataaac
     2641 cgaacgaacc gattctttca gggctgtcgt tcgaggtgaa acccggcgaa gtcatcgcgc
     2701 tcgtgggccc ctcgggcgcg ggcaagagca ccttcgtcaa cctgattccc cggttcttcg
     2761 acgtgcaggc gggccgcatc accatcgacg gcatcgacgt gcgcgacttc cgtttgtcag
     2821 atcttcgcgg tcacatcggc atcgttccac aggaaacact gttgttttcc ggtacaattg
     2881 ccgagaatat ccgcctgggt cggctcgacg cctcgcagac cgagatcgaa gaagcggcac
     2941 gtctgtcgaa cgctcacgac ttcattcact cgtttcccga ggggtacaat acgtatctgg
     3001 gggaacgcgg ggtcaatctc tccggcggac aggctcagcg tatcgctctg gcgcgcgcct
     3061 ttctgaaaga ccccccgatt ctgattctcg atgaagccac gtcggccctg gattccgagt
     3121 ccgagaatct ggtgaaagat tcactgaaca aactgatgcg caatcgtacg accttcctga
     3181 tcgctcaccg gctgtcgacg atcatcaacg cggacaagat cgtcgtgctg cagaatggcg
     3241 gcatcgtcga atgcggcccg cacaagcaac tgctcgccga tcaggggttg tattcgcagc
     3301 tgtatcacgc gcagtatgcc catgaaacca tgccacaggg gaggatgcat gcccactgat
     3361 ctcacgcatg aatccgatct tgaagcccgc tgggacgaac ccggctatgc gcagaaggcc
     3421 cgcatcaaag gccgctgtgc cggcctgttc gtcaagctgt gggcctcgag cctgcgtgtc
     3481 gagcacatcg gcgccgaccg ggctcccctg ctgcatgcgg ccggaaggcg ggtgctgttc
     3541 gtggtctggc acggctcgca gctggtaccg ctggccacgt atcgcaaccg gggcatttcg
     3601 atcatgacca gtctttcgcg ggatggcgac attcaggatt cgtgcatgaa gacgctcggt
     3661 ttcacgacgg tgcgcggttc ctcttcgcgt ggtggggcgc gggcactgct ggccatggtg
     3721 cgcagccttg aaaaaacggg tctggcggcg atcgccgtcg acggtccccg aggcccctat
     3781 catcaggcca aacccggagc cgtcgtgctg gctcagaaga cgggggccat cgtcctaccc
     3841 gtgggtgcgg ctcacacccg ctgtcgacac ttgcgcagct gggacaaatt cgaactgccg
     3901 ctcccgtttt cccgctcggt cgtctggacc ggcgagccct tcgagatgcc cgcccgctgc
     3961 agccccgaag aggggtgccg cctgatcgaa gaacacctgc agcgctgcga agaccaggcg
     4021 cgcgaaagga tcgccgcatg acccagagca ccgaacgcaa gcggattctc attgtcgccg
     4081 gcgaaacgtc gggcgacatg tatgccgccg cgttggtcga cgaactcgaa cgcctgggtg
     4141 agttcgagat cttcgcggtt ggcgggccgc aaaccgccaa gcgcaaggtg aatctgctgt
     4201 atgacagttc ggactgggcg gtcatcggct acgtcgaagc catcaagcgc gctccgcagc
     4261 tgtatctggt tctgcgagat ctcaagcggt ttctggagca gcggcgaccg catctggtcc
     4321 tgctgctcga ctacccgggg ttcaacatgc ggctggccaa gcatgccaag agcctgggaa
     4381 ttccgacgct gcattatttc ccccccagta aattcgccac gaccccggaa gaagtggctg
     4441 atgccgcctc gacgctgacg tgtgtcgccg ccaacttcac gtttacctac gagaattaca
     4501 aagccgcgaa cgccaacgtg cacttcgtcg gacacccgct ggtcgatctg gccaaaccca
     4561 ccatgagccg ggaagaagcc ttccgcactt tcggtctcga cccggcccgg ccggtcgtcg
     4621 ggttgtgccc gggcagtcg
//
LOCUS       DAORHD010000204         4632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19623_length_4632_cov_3.758357, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000204 DAORHD010000000
VERSION     DAORHD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19623_length_4632_cov_3.758357"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKO06_05690"
     CDS             complement(<1..371)
                     /locus_tag="PKO06_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69171.1"
                     /translation="MNFQSLLTWITGRTPPEPPQKTVASEPASVERRRSSRLPISEGE
                     AVIAEHPPFPISDVSFRGIRFEVPDGNKPAVWNIGTQLSATLKFGTVRIASQLKICNL
                     WPTAVGCLFTGIAPHQSLRLA"
     gene            512..2329
                     /locus_tag="PKO06_05695"
     CDS             512..2329
                     /locus_tag="PKO06_05695"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNV69172.1"
                     /translation="MLNNVEREKLKTSPAVTATPESDQGRVSRRASGLLLLASLVVGF
                     GLFLMKQDFAATCDAAFHLRYARDLFLAILDGAGYPDWDALPFDGRGTAAFRYYAPLP
                     YLVAGVLQILGLGPVTAVKGTMLLFAMIAAWGVRSWFGTLRREADGVAAAAILLCSPI
                     LTFHLFYVFLFQNLCAMCLLPWLLAAMNRYWREEHAGFAKAGIAFGCMFLTHLPSALI
                     AGYTLLLLGLLEFWKQRRILPLVRLGLVAAVGLLLAAPYVLPSMLTLSHIHFQRGMVS
                     LAPGTGTECLDDPFLTTAHRRISGWEALGQLASLCRQRCLGQATMPTSAADESAPKLA
                     NDNQLYPFTQVRPWLWFCWLSMILAVVPGAVLSYRREHWWPGMIGMILLVLSLRMTNP
                     LFSVLPGLSVLQFAWRLLLPAHVLLLPWMVCIAADLRTRRLAVLALCPAVLLTVGMQL
                     VSGTFTPPMVESFFRVPAYPWEYLPTACPDPERLSPLAGKPHRLQPSSAITNLEELER
                     GFESLVFRAEVASGGGELFIQTHFDPGWMLCVGDEETPLDGGGACGTMRARLPGGRIV
                     CRLTRRAPAGRWAGWLGMLLGAGWLFVASRREMRPADVA"
     gene            complement(2449..3852)
                     /locus_tag="PKO06_05700"
     CDS             complement(2449..3852)
                     /locus_tag="PKO06_05700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV69173.1"
                     /translation="MSQTPQATSEPRSGTDLSVGSIPRHLITFSIPLLMGSLIQTAYG
                     VINAIWVGHGLGPQALGAVTCSFPVTFLFIAVSGGLTMGASILVSQRYGARDYDGVRT
                     VVQTAFWLTLLVAALCFTAGQLLVDRPLHLMQTPAEILPLASSYLKLYLCTTPSMFLL
                     FLLVSALRGTGDARTPLRYQFWALLATAVLDPFLMFGWLGLPRFGLNGTAIAAIVCQI
                     GSVVLLYHELHRRRHLVSPDLRQRNVDGPTARQMLRIGIPSMIQQGLVSVGMLVIISM
                     VNTFGKDATAAFGAASRFDQVTFLPAMTIGMATASLAGQNIGAGKFDRVRQTFRWAAA
                     LSVSITFPAACVAYFFPDFILGLFISDPVVTELGRSYLRIIAPGSLFFSIMFASNGVI
                     NGAGHTIATTFFTLIGMWIVRVPLAWWLSHRWNSVDGVWWGITLGFIGGMFTSLGYYL
                     LGRWQKPTRATPPRPSS"
     gene            3980..>4632
                     /locus_tag="PKO06_05705"
     CDS             3980..>4632
                     /locus_tag="PKO06_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69174.1"
                     /translation="MRVRSLLILILFCTLFAGSSSALENGYREPSERVKRVALAPPTP
                     TLVINPRGGTALIVRTEDQTDLAELFQPLMRLAGVRFYPLSRTKQRFSFVVGISILDL
                     ASREEKTVALPAGTRWGFPSWSPDGTRWAMTLYEKDQACVWWFDATTRRGTALPQPVS
                     NLLLSPFAWVDATHLLLPTVPVGQGSAPEPLPYPASPNVQESAGKVAQVRTFQDLLRT
                     "
BASE COUNT          898 a         1297 c         1472 g          965 t
ORIGIN      
        1 tcggccagtc tcaggctttg atgtggagca ataccggtga agagacaccc aacggccgtt
       61 ggccacagat tgcagatctt caactgcgag gcgatacgaa cagttccgaa cttcagggtc
      121 gcggaaagct gggtgccgat gttccagacg gcaggcttgt ttccgtccgg cacctcgaag
      181 cgaattccgc gaaagctcac gtcggaaatg ggaaatggcg gatgttcggc aatcacggct
      241 tcgccttcgc tgatgggcag gcgcgaactg cgacgacgct cgaccgaagc gggttcgctt
      301 gccaccgttt tctgaggcgg ctccgggggc gttcgtcccg tgatccacgt gagcagggac
      361 tgaaaattca tatcctgggt tggcccttcc aatgatcctg cttttctata ccacaaaaag
      421 cctgcgattt gctcgatcgg cctgtgaata ctccagggtc tgcggcgttg acacgcgcgg
      481 gtgcatcggg tagggtgcaa agacgcatca aatgctgaac aacgtggaga gagagaaact
      541 gaaaacgtcg cctgcggtga ccgcaacgcc agagtctgac cagggccggg tctcgaggcg
      601 agcctcggga ttgctgttgc tggcgtcgct cgtcgtgggt tttggtctct ttctcatgaa
      661 acaggatttc gcggcgacct gcgatgcggc atttcatctg cggtatgccc gcgacttgtt
      721 tctggcgatt cttgacggag ccgggtaccc ggactgggac gccctgccct tcgatggccg
      781 aggcactgcc gcgtttcgct actatgcccc gcttccgtat ctggtcgcgg gagtcttgca
      841 gattcttggc ctggggccgg tgaccgcggt caaaggaacc atgctgttgt tcgcgatgat
      901 cgccgcctgg ggagtgcgat cctggttcgg caccctgcgg cgggaagcgg atggcgtggc
      961 ggcggcggcc attctgctct gttcccccat tctgacgttt catctgttct atgtgtttct
     1021 gtttcagaac ctgtgtgcca tgtgtctttt gccgtggctg ctggccgcca tgaaccgata
     1081 ctggcgagaa gagcacgccg gcttcgcgaa agccgggatt gcctttggct gcatgtttct
     1141 tacacacctt ccttcggctc tcattgccgg ttacacgttg ttgcttctcg gactcctgga
     1201 gttctggaag cagcggcgaa tattgccgtt ggtgcgactg ggtctggtcg ccgctgttgg
     1261 gttgctgctg gcggcaccgt atgtgctgcc ctcgatgctg actctctctc atattcattt
     1321 tcagcgaggc atggtgtcac tcgctcctgg taccggtacc gagtgtctcg acgatccatt
     1381 cctgacaacc gctcaccgac gtatttccgg ctgggaagcc ctgggacaat tggccagcct
     1441 ctgccgacaa cgttgtctgg gtcaggctac gatgccgact tctgcagcag atgagagtgc
     1501 gccgaaactc gcgaacgaca atcaattgta cccgttcaca caggtgcgtc cgtggctgtg
     1561 gttctgctgg ttgtccatga ttctggctgt cgttcctggt gcggttctct cctatcggcg
     1621 agaacactgg tggccaggca tgattggcat gatactgctc gtactatcac tgcgcatgac
     1681 taacccgctc ttttcggtgt tgccgggatt atcggtgctg cagttcgcct ggcgtctgtt
     1741 gctgccggct catgtgctgc tgctgccctg gatggtctgc atcgcggcag acctgcggac
     1801 gagacgcctg gccgttcttg cgctctgtcc cgccgtgctc ctgacggtgg gaatgcagct
     1861 cgtcagtgga acctttacac caccgatggt cgaatcattc ttccgtgtgc cagcctaccc
     1921 gtgggagtat ctgcccaccg cctgccctga tccggagcgg ctctcgccgt tggccggaaa
     1981 accccatcgt ctgcaacctt cgtcggccat cacgaacctt gaagagctcg aacgcggctt
     2041 tgaaagcctg gtctttcgtg ccgaggtggc ctcgggcggt ggcgagctgt ttattcagac
     2101 gcatttcgac cctgggtgga tgctgtgtgt cggagacgaa gaaacacccc tcgatggtgg
     2161 tggggcatgt ggcaccatgc gggcgcggct tccgggaggg cggatcgtct gccgtctgac
     2221 gcgtcgagcg cctgcggggc gttgggccgg atggctggga atgcttttgg gagccggctg
     2281 gttgttcgtg gcatcgcgtc gagagatgcg accggcggat gttgcctgac ctggcagcgg
     2341 cgggaaccgt caggagtggc gcatcaaaag aaggtgaacg aagagtacaa gtcgcaccag
     2401 gctgtcatga agagaaaaag gctttgtgcg gccagataaa cagccgactc agctcgaggg
     2461 acgtggcggt gtcgcccgcg tcggtttctg ccagcggccg agcagataat atccgagact
     2521 ggtgaacatg ccgccaatga agccaagggt gatgccccac cagacgccat cgaccgagtt
     2581 ccatcggtgt gaaagccacc aggcaagcgg tacgcgaacg atccacatgc cgatgagagt
     2641 aaagaaagtc gtggcgatcg tatgcccggc accgttgatg acgccattac tggcgaacat
     2701 gatggaaaaa aacagcgaac ccggagcgat gatgcgcaga tagctgcgac cgagttcggt
     2761 gaccacgggg tcgctgatga agagccccag aatgaaatcg ggaaaaaagt aggcgacgca
     2821 cgcggcggga aaagtgatgc tgacgctgag tgccgcggcc cagcgaaacg tctgacgaac
     2881 ccgatcgaat tttcccgcgc caatgttctg cccggccaga gatgcggttg ccataccgat
     2941 cgtcatggcc ggcagaaagg tgacctgatc gaatcgtgaa gcagcgccga aagccgccgt
     3001 ggcatccttg ccgaaggtgt tgaccatgct gatgatgacg agcatgccga ccgacacgag
     3061 accctgttga atcattgagg gaatgccgat acgcaacatc tgtcgggcgg tcggtccgtc
     3121 aacgtttctc tgcctcaggt cggggctgac cagatggcgc cggcggtgga gctcatgata
     3181 gagcagaacc actgacccga tctggcagac gatggctgcg atcgccgtgc cgttcagacc
     3241 gaaccgcggt agacctagcc agccgaacat cagaaacggg tcgagcacgg cggtggcgag
     3301 cagagcccag aactgatatc gcagcggtgt ccgggcgtcg cctgtcccgc gcagagccga
     3361 caccagaaga aacagcagga acatcgaggg cgtggtacag agatacagct tcagatacga
     3421 agaggcgaga ggaaggatct cagcgggtgt ctgcatcaga tgcagcggtc gatcgaccag
     3481 aagttgtccc gcagtgaagc agagtgccgc cacgagcaga gtcagccaga atgcggtttg
     3541 tacaaccgtg cgcacgccgt cgtaatcgcg cgcgccgtac cgttgcgaga ccagaatcga
     3601 agcgcccatg gtcaggccgc ccgaaacggc gatgaagaga aacgtgacgg gaaagctgca
     3661 cgtgacggca ccgagcgcct gtggcccgag gccgtggccg acccagatgg cgttgatgac
     3721 tccataggcg gtctgaatga gactgcccat gagcagtgga atcgagaagg tgatcagatg
     3781 ccgcggaata ctgccgacgg agagatcggt tcccgaacgg ggctctgaag tggcttgagg
     3841 cgtttgagac atgcgcccga agcgtaccag attctcgcga aaaatacatc cagtctgttt
     3901 gcctgcgggc ctgaccggaa ggttgccgat acgtgggaac tctgatattc aacaggaata
     3961 ccaacgaggg agatccccca tgcgcgttcg atcgcttctg attctgattc tgttctgtac
     4021 cttgtttgca ggctccagca gtgcgctaga aaacggatac cgggaaccgt ccgaacgtgt
     4081 gaagcgcgtg gcgctggcac cgcccactcc gacgttggtg atcaaccctc gtgggggaac
     4141 ggcgttgatc gtgcgcacgg aagatcagac cgacctcgcg gagctttttc agcccctgat
     4201 gcggttggcc ggtgtgcggt tctatccgct gtcgcggacg aaacagcgct tctcgttcgt
     4261 ggtgggcatc tcgattctgg atcttgcctc gcgcgaggaa aagaccgtgg cgttgcctgc
     4321 gggtacgcgc tggggattcc ccagttggtc acctgatgga acccgatggg cgatgactct
     4381 ctatgaaaaa gaccaggcgt gtgtgtggtg gttcgatgcg accacccggc gtggcacggc
     4441 tctgccgcag cccgtgagta acctgctcct gtcgccgttt gcctgggttg atgcgacgca
     4501 ccttctgttg ccgacggtac cggttggaca gggctcagcc cctgaaccat tgccgtaccc
     4561 agcaagcccg aatgtgcagg aatcggctgg caaggtcgct caagtcagaa cctttcagga
     4621 tctgctgcgt ac
//
LOCUS       DAORHD010000205         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19640_length_4630_cov_4.247213, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000205 DAORHD010000000
VERSION     DAORHD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19640_length_4630_cov_4.247213"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PKO06_05710"
     CDS             complement(<1..1090)
                     /locus_tag="PKO06_05710"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNV69175.1"
                     /translation="MGNRDQRRKLKRQVNTSRIGWVFLLVLAVTTMSAAAQSLSDRAR
                     LVLESTSRQDFPIAVRTGRETTGNVYREGENLQISILASITAYLQVFATSPDGDIVQL
                     FPESATADTRIAARSETRVPPDRSRFALQMGKTAGTDLVTVVVTDRNHPLLSPSDLKR
                     AGNALLVRDPDRLAALLQRLDADTSCKACIQHVPIHTSAPALSSGGFGTASSTDGWRA
                     HLWVRRDPVPADEPIVVRVVSNQNCLVKSVLVGDTSTSPQTALPLKHSPVVEHGRIVH
                     IPSASDTFSIRLLPPHNRAIMHVTLQRSNGENLQVSCPISSTWTASSAPQPPVTGKPH
                     QTAGASGSATPSSTIYPHQPRNDWQGSLA"
     gene            complement(1108..2361)
                     /locus_tag="PKO06_05715"
     CDS             complement(1108..2361)
                     /locus_tag="PKO06_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69176.1"
                     /translation="MICQACAATFWLPDDYVKVRIKCPKCSKWADIPDSVRTLAKLEP
                     RLARVKFVRVKSRLDQEGLMDSHSSTALASTLTRFTDVSRLFLIDELPSMARTLSADN
                     LWESLKLAERLQEQSAESCSWWDYYSGSVVDSYLAVWYRLMMRLGFTTLAGLVEKQLS
                     QSISAPYEMIFWKTLATIHDNLGPAGFVDYQDWLKELSQARAREKSGDALNDEQFYRF
                     HLDAMERLPNLMERYSVPDCRAVMKRISAVSLSESTILGDYSGIDTDQYAAFLGCHIS
                     LAQKPESYSVAMRLGNEALAASALVQAQQAYERALAFAPKSDACRYNLGLVYTKQSRY
                     AEAIESLKQAVELHPRHVKAWYMLGLAFKKAGDREQARKALEEGRDLNETWRYLASLP
                     AGHLSYPLQAAEDLKPEFDDLLGQL"
     gene            2840..>4630
                     /locus_tag="PKO06_05720"
     CDS             2840..>4630
                     /locus_tag="PKO06_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV69177.1"
                     /translation="MRQQPSVRERLQYRFDNFMARGFGALILALGVASAAVILFAALV
                     VVITGAAPEGESVPGFIEAAWLSLMRTLDAGTMGGDKGWGFRFIMFAVTMGGIMVIST
                     LIGLISGAIEEKVEELRRGRSRVLEEGHYVILGWTPAVFTIVQELITALKPESRSCIL
                     VFSKTDKVEMEEEIAKRISDYGNTMVICRNGDPVDPDDLALANLDNSKSIVILTGEGD
                     NPDADVIKTILAITQAKTRRPEPYHIVAEIVNPKNVEVARSISPQELEIVQTAETLSR
                     IIAQTCRSSGLSLVYSELLGFDGDEIYFKEIPEMVGKTFGDSIMWFSDSCIIGMLPQG
                     GNPQMNPPMDTPIKPGDQLIAITKDSTSLKSLRGGPPAVQAPVPDTTQPQHPTPEHTL
                     ILGYNWRVPAVIQQLDEYVAEGSTVTLVASTGKAAEEVGVECGKLKKCKVDVRTADTT
                     DREVLEEVLQKRFNRVILMGYTDEMSQQEADSQTLISLLHLRDLQKKLGCKLGVVTEM
                     LDQRNRVLADVAHADDYIVSSHLVSMLLAQVSYKKEVHPIFIDIFDPEGSEIYLKLAS
                     RYCQLGQPVDFFTISESARRKSEIAIGYRIK"
BASE COUNT         1014 a         1254 c         1356 g         1006 t
ORIGIN      
        1 tggcgagcga gccctgccag tcgttgcgcg gctgatgggg atagatggtc gaggaaggag
       61 tggcagaacc acttgcacct gctgtctggt gaggtttgcc tgtcacaggc ggttgtggcg
      121 ctgatgaggc tgtccaggtg ctgctaatcg ggcaggaaac ctgaaggttt tcgccgttgg
      181 aacgctgaag cgtgacgtgc attatcgccc ggttgtgtgg cgggagaagc ctgatggaga
      241 aagtatctga cgcactcgga atatggacga tgcgcccgtg ttcgacaacg ggggaatgct
      301 tcagcgggag agctgtctgt ggcgaagtcg atgtatcacc gaccaaaacg cttttcacga
      361 ggcaattttg attgctgacc actcggacaa cgatcggctc atctgccgga acggggtcac
      421 ggcgaaccca gagatgcgcc cgccacccgt cggttgacga agccgtgccg aaaccgccgc
      481 tcgataaagc aggtgcggag gtgtgaatag gtacatgctg gatgcatgcc ttgcatgaag
      541 tgtcggcatc gaggcgttga agcaaagcgg cgagccggtc cgggtcacgc actagcaggg
      601 cgttccctgc acgtttcaga tcagatggcg ataacagagg gtggttccgg tcggtgacga
      661 caacggtcac gagatctgtc ccagcggtct tacccatctg cagggcgaaa cgcgacctgt
      721 cgggtggtac tcgggtttcg cttcgcgcag caatgcgggt atcggctgtt gcgctttccg
      781 gaaagagctg aacgatgtcg ccgtcgggcg aagtcgcgaa tacctgcaga tacgcggtga
      841 tcgaagccag aatcgaaatc tgcaggttct caccctctct gtacacgttt cctgtcgttt
      901 ctcgaccggt acgaacagcg atcggaaaat cctgccgcga ggtgctctcg agcaccagtc
      961 tggcgcgatc cgaaagtgat tgagccgccg ctgacattgt ggtgacggca agaaccaaca
     1021 gaaacaccca gccgatacgg ctcgtgttca cctgacgttt gagctttcgc ctctggtctc
     1081 gattgcccat gcctctccta ccgttcgtca cagctgtccc agcaaatcgt cgaactccgg
     1141 cttcagatct tctgcggcct gaagcggata actcagatgc cctgcaggca aagaggccag
     1201 atatcgccag gtttcgttga gatcgcgacc ctcttccaga gcttttcgag cctgttcccg
     1261 gtcgcccgcc ttcttgaagg ccaggcccag catataccag gctttcacgt ggcgggggtg
     1321 cagttccacg gcctgtttca gcgattcgat cgcttctgcg taccggctct gcttcgtgta
     1381 aaccagcccc agattgtagc gacaggcatc ggactttggc gcgaatgcca gagcgcgttc
     1441 atacgcctgt tgagcctgaa ccagagcgga ggccgccaaa gcttcgttgc caagccgcat
     1501 ggccacggag tatgactctg gcttctgcgc aagggaaatg tgacaaccca aaaacgcggc
     1561 atactgatcg gtgtcgatgc ctgagtagtc gccgagaatc gtgctttccg agagtgagac
     1621 ggcgctgatt cgtttcatga cggctcggca atcgggcacg gaatagcgtt ccatcaagtt
     1681 cggaaggcgt tccatggcgt cgagatgaaa gcgatagaac tgttcatcgt tcaaggcgtc
     1741 accgctcttc tcacgtgctc gtgcctgtga caactccttc agccagtcct gataatccac
     1801 gaatcctgcc gggccgagat tgtcatggat tgtcgcgagc gttttccaga agatcatttc
     1861 ataaggggcc gagatggatt gcgagagctg tttttcgaca agtccggcca gggttgtgaa
     1921 ccccaggcgc atcatgagtc gataccacac ggccagataa ctgtcgacga cggagcctga
     1981 gtaatagtcc caccatgagc aggattccgc tgactgttct tgcaggcgct ccgccagttt
     2041 caaagattcc cacaggttgt cggcagagag cgttcgagcc atagagggaa gctcgtcgat
     2101 gagaaacagt ctgctgacat cggtgaagcg ggtgagtgtc gatgcgaggg cagtgctgga
     2161 atgcgagtcc atgagtcctt cctggtccag acgggactta actcggacga acttcactct
     2221 tgccaatctc ggttccagtt tggccagagt tctgacggag tctggaatgt cagcccactt
     2281 cgagcacttc ggacatttga tgcgcacttt cacgtaatcg tcgggaagcc agaaggttgc
     2341 cgcgcaggct tggcagatca tgggagcgcc ggcatgaacc tccgactgac attcggaaca
     2401 tcggagattc gcatggtcga tatcgtccgg gattccatca aatgatccgc attgaagaca
     2461 ctgataggag tacatacgtg attgctccga aagacgtttt gctcattgat cgctctctga
     2521 agaacagcca tgcgcttctg ttgtttagag cgaacagcgt tcggggttta ttctaccatc
     2581 gattctcgaa tcgttgcacc atctcggaac gacgtcgtcg caacaggcgt cgctacggga
     2641 agtgcccgca ctatgctgtc ggatggtact ctacgacggg gaaggcgtag agcgatggca
     2701 atgaacaccc ttagacaacg atctgagtga tttcacggag tgatggaaat aaggtggcac
     2761 aagtcggagc gggtggtata gtgacaccgt cagatcagcc ttttctgctg atcgcatttt
     2821 gtaccgtgcg aggaattctg tgagacaaca gccgtccgtg cgtgagcgct tgcaataccg
     2881 tttcgacaac tttatggccc gtgggttcgg agcgctgatt ctggctctgg gtgtcgcctc
     2941 cgcggcggtg attctgtttg ccgccttggt ggtggtgatc acgggggcgg ctccggaggg
     3001 tgagtctgtt cctggattca tcgaagcagc ctggctgagt ctcatgcgca cgctcgatgc
     3061 gggaaccatg ggcggtgaca aaggctgggg tttccgcttc atcatgtttg ccgtcaccat
     3121 gggcggcatc atggtcatca gcacgctgat cggtttgatc agcggggcga tcgaggagaa
     3181 agtcgaagaa ctgcggcgcg gacggtcgcg cgttctcgaa gaagggcatt acgtgattct
     3241 cggctggact cccgccgttt tcacgatcgt tcaggagctg atcaccgcat tgaagcctga
     3301 atctcgctcc tgcatcctgg tcttcagcaa gaccgacaag gtcgagatgg aagaggaaat
     3361 tgccaaacgt atctcggatt acgggaacac catggtcatt tgtcgcaacg gagacccagt
     3421 cgatcccgat gatctggctt tggcaaacct ggacaattcg aagtccattg tcattctgac
     3481 cggcgaaggc gacaatcccg atgccgatgt catcaagacc attctggcca ttacgcaggc
     3541 gaagacgcga cgaccggaac cgtatcacat cgtcgcggag atcgtgaacc cgaagaacgt
     3601 ggaagtggct cgttccatct ctccgcagga gctcgaaatt gtccagacgg ccgaaaccct
     3661 gtcgcggatc atcgcccaga cctgccgcag ttccggcttg tctctggtct acagcgaact
     3721 gctcgggttt gatggtgacg agatctattt caaggaaatt cccgaaatgg tgggaaagac
     3781 ctttggtgac agcatcatgt ggttcagcga ttcctgcatt atcggcatgc tgccccaagg
     3841 cgggaatccc cagatgaatc cgccgatgga taccccgatc aaaccaggcg atcagctcat
     3901 tgcgattacg aaagacagta ccagcctcaa atcattgcgt ggcggtcctc cggcggttca
     3961 ggcaccagtc cctgatacca cccaacccca gcacccaacc ccggaacata cgctgatcct
     4021 cggatacaac tggcgcgttc cagctgtcat tcaacagctc gatgaatacg tggcagaagg
     4081 gtcgacggtg accctcgttg cctccacggg caaggcggca gaagaggtgg gtgtcgagtg
     4141 cggcaagctg aaaaagtgca aagtggatgt cagaacagcc gacactaccg accgtgaagt
     4201 cctcgaagaa gtgctgcaga agcgtttcaa ccgagtcatc ctcatgggct ataccgatga
     4261 aatgtcccag caggaagcgg actcccagac gctgatttcc ctgctgcatc ttcgcgatct
     4321 gcagaagaaa ctcgggtgca aactcggcgt cgtgaccgag atgctcgatc agcgcaaccg
     4381 cgttctcgcc gacgtcgccc acgccgacga ttacatcgtc agcagtcacc tggtgagcat
     4441 gcttctggcg caggtttcct acaaaaaaga ggtgcatccg atctttatcg acatctttga
     4501 tccggaaggc tcggaaatct atctcaagct ggcttctcgc tactgccagc tcggacaacc
     4561 ggtggacttc tttacgatct ccgaaagtgc tcgacgaaag agtgaaatcg ccatcgggta
     4621 tcgcatcaag
//
LOCUS       DAORHD010000206         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19672_length_4625_cov_4.907877, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000206 DAORHD010000000
VERSION     DAORHD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19672_length_4625_cov_4.907877"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="metG"
                     /locus_tag="PKO06_05725"
     CDS             <1..1344
                     /gene="metG"
                     /locus_tag="PKO06_05725"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HNV69178.1"
                     /translation="RLKWGIDLPFDPDYVTYVWFDALTNYISAIGYPSDQARFSKHWP
                     ASCHLIGKDILTTHSVYWSTMLFALELPLPECIFAHGWWISSGGKMSKSVGNVVNPLD
                     LIDKYGLDPFRYFLFREMVFGSDAAFSEEAFILRYNADLANDFGNLCHRTLPMLIKRR
                     EGIFHKNQPVLPPTKEIAELATAVKADYVQAMENFLFHEALKRTWDLVSRLNKYVDEC
                     APWALAKNEKWPELDEVFYTVGEGLRFIAHLVNPFMPHIAPKFLEQIGLKPSFLPLND
                     LAWGQLAEGTRCAEPVPMFPRIETERIEALKQGAKPIEKAKETKEPKEVKAAKPVKPD
                     PNAVPEPIDFDEFLKVQLVVAEVLTAEKVENADKLLKLTVKIGDETRSLVAGIAAQYS
                     PEQIKGKQVVMVKNLKPAKIRGIVSEGMILAAGTPDGGLAVCVPDRPATCGVRVK"
     gene            1362..2138
                     /locus_tag="PKO06_05730"
     CDS             1362..2138
                     /locus_tag="PKO06_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV69179.1"
                     /translation="MRLIDVHAHLQDPAFDTDLGEVLQRAAEAGVTATVLPGTTMADS
                     RRAVQLAERHHGLWAMVGVHPHEAKDWESTALTELEEMLKHPKVLGIAEIGLDFHYAF
                     STPEQQRLALTEQWCLAARLGAPVEVHVREAFPEFFGTVAQLPPPPRVMLHCYSGGFD
                     ETKIAVKQGWHFSIGGSLTFKKAEECRAIFAELPADRVHLETDCPYLSPEPFRGRRNE
                     PGNLSHTLQRLAEVRRQTHDTLADQLWKNARDFFGPRLHP"
     gene            2138..3229
                     /gene="aroB"
                     /locus_tag="PKO06_05735"
     CDS             2138..3229
                     /gene="aroB"
                     /locus_tag="PKO06_05735"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNV69180.1"
                     /translation="MLESQKISIKRDLSYEIALGSEVLPLLVGKIARLGFTKRAIILT
                     NDVVSRWYLEPLVRELEHRGFTATTVIVPDGESYKSLETAQQTYPKLIEAGVDRSSPI
                     ITLGGGVIGDLGGFLAATYMRGIPFVQLPTTLLAMVDASIGGKVAVNLPAGKNLIGSF
                     YQPSFVGIDVSTLKTLPLTQISYGIVEAIKHGAICDPAYYKFIYKNRAAIKNRDLLLV
                     QRLVRRSLHLKKNVVEQDEQDQGLRHTLNFGHTFGHALETLGEYRRYHHGEAVGIGML
                     LALKAAKALHRLQEDYSESLRELLLDFNLPVDPPREWGPEQFTKAIAHDKKALKDKIK
                     FVLPLALGKVEVVSVPAADVPGLFEQILG"
     gene            3269..>4625
                     /locus_tag="PKO06_05740"
     CDS             3269..>4625
                     /locus_tag="PKO06_05740"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNV69181.1"
                     /translation="MPHSPVSASLSGFTPNAQKILAKRYLTKNEDGTLKETIPELFHR
                     VARAIAEAESRFSHSPFSVEQLADKFYQMMIAQEFMPNSPTLMNAGKPLGQLSACFVL
                     PVGDSLDSIFDSIKFAAMIHKSGGGTGFSFARLRPANDIVATTNGVSSGPVSFMRVFN
                     IATETIKQGGTRRGANMGILRVDHPDILDFIKSKEANDSLNNFNISVAITDRFMEALA
                     KGEDYELFNPRTNDVVKKLNARKVFNLIVEGAWRNGEPGIIFIDRINRDNPTPQVGLI
                     ESTNPCGEQPLLPYESCNLGSLNVAKFLIADGAFSRIDYPRLKQAIHLSIRFLDNVIE
                     VNRYPLPDIEQMTKANRKIGLGIMGWSDLLIRLRISYNSEMALNTAEQLMSFIQRESW
                     AASAQLAQERGHFANFPGSELEKRGMSPVRNATVTTIAPTGTISMIANCSSGVEPLFA
                     IA"
BASE COUNT          961 a         1523 c         1298 g          843 t
ORIGIN      
        1 cgcctcaaat ggggcatcga cctgcccttc gacccggact atgtcaccta cgtctggttc
       61 gacgcgctga ccaactacat cagcgcgatc gggtacccta gcgatcaggc gcggttcagc
      121 aagcactggc cggcctcgtg ccacctgatc ggcaaggaca ttctgaccac gcacagcgtc
      181 tactggtcga cgatgctgtt cgcgctcgaa ctgccgcttc ccgagtgcat cttcgcgcac
      241 ggctggtgga tctcgagcgg cggcaagatg tcgaagtcgg tcggcaacgt ggtcaacccg
      301 ctcgacctga tcgacaaata cgggctcgac ccattccgct actttctctt tcgcgagatg
      361 gtcttcggca gcgacgccgc cttttcggaa gaagccttca ttctacgcta caacgccgac
      421 ttggccaacg acttcggcaa cctctgccat cgcactctgc ccatgctcat caagcgtcgc
      481 gaaggcattt ttcacaagaa ccaaccggta ctgccgccga cgaaggagat cgccgagctg
      541 gctacggccg tcaaggccga ctacgtgcag gccatggaga acttcctgtt tcacgaagcc
      601 ctgaagcgca cctgggatct cgtcagccgc ctgaacaagt atgtcgatga gtgcgccccg
      661 tgggcgctgg cgaagaacga gaagtggccc gaactcgacg aggtcttcta caccgtcggc
      721 gaagggctgc ggttcatcgc ccacctcgtg aaccccttca tgccgcacat cgccccgaag
      781 ttcctcgagc agatcggact caagccttcg ttcctgccgc tgaacgacct ggcctggggt
      841 cagctggccg aaggcacgcg ctgcgccgag ccggtgccga tgttcccacg catcgaaaca
      901 gagcgcatcg aagccctgaa acagggagcc aaaccgatcg agaaggccaa agagacgaag
      961 gaaccaaagg aagtaaaagc ggccaagccc gtcaagcccg acccgaacgc cgttccagag
     1021 ccgatcgatt tcgacgagtt tctcaaggta cagctggtcg tcgccgaagt gctcaccgcc
     1081 gaaaaggtcg agaacgcgga caagctgctg aagctcactg tgaagatcgg cgacgaaacg
     1141 cgttcgctcg tcgcgggcat cgccgcgcag tattcgcccg agcagatcaa gggcaagcag
     1201 gtcgtcatgg tgaagaacct gaagccggcg aagattcgcg gcatcgtctc ggaaggcatg
     1261 atcctggccg ccggcacccc cgacggcggg ctggctgtct gcgtacccga tcgccctgcc
     1321 acctgcggcg ttcgcgtcaa gtagtcacga tcggtcgctc gatgcgcctt atcgacgttc
     1381 acgcccacct gcaggacccc gccttcgata ccgatctcgg cgaggttctg cagcgagcag
     1441 cagaagccgg agtcacggcg accgtgctgc cgggcacgac catggccgat tcacgtcgtg
     1501 ccgtgcagct cgccgaacga caccacggtt tgtgggcgat ggtgggcgta catcctcacg
     1561 aagccaaaga ctgggaatcg acggccctca ccgaactcga agagatgctg aaacacccga
     1621 aggtgctcgg tatcgccgag atcggtctcg actttcacta cgccttttcg acacccgaac
     1681 aacagcgact ggccctgacc gagcagtggt gcctcgcggc ccgcctcgga gcgccggtcg
     1741 aggtgcatgt acgcgaggcg ttccccgagt ttttcggcac cgtcgcccag ctgccgcccc
     1801 caccccgcgt catgctgcac tgctactcgg gcggctttga cgagacgaag atcgcggtca
     1861 agcagggctg gcatttctcg atcggcggct cactgacctt caaaaaagcg gaagagtgcc
     1921 gcgccatctt tgccgaactg cccgccgatc gcgtgcatct cgagaccgac tgcccctacc
     1981 tgtcccccga gcctttccgc ggtcgtcgca atgagcccgg aaacctctct cacacgctgc
     2041 aacggctggc cgaggtgcgc cgccagacgc acgacacgct cgccgatcag ctgtggaaaa
     2101 acgcccgcga ctttttcggg ccccgccttc acccctgatg ctggaatcac aaaaaatatc
     2161 gatcaaacgt gacctgtcgt atgaaatcgc gctcggcagc gaggttcttc ctctgctcgt
     2221 cggcaagatc gcccgcctgg ggttcaccaa gcgcgccatc attctgacca acgacgtcgt
     2281 ttcgcgctgg tatctcgagc cactggtgcg agaactcgaa catcgcgggt tcaccgcgac
     2341 gacggtgatt gtcccagacg gtgagagcta caagtcgctc gagacggcgc aacagacgta
     2401 cccgaaactc atcgaggcgg gcgtcgatcg cagctcgccg atcatcacgc tcggcggcgg
     2461 tgtcatcggt gatctgggcg gctttctcgc cgccacctac atgcgcggca ttcccttcgt
     2521 tcaactgccg acgacgctgc tggccatggt cgacgcctcg attggcggca aggtcgccgt
     2581 gaatctgcca gccggcaaga atctgatcgg ctcgttctac cagccgtcgt tcgtcggcat
     2641 tgatgtctcg acgctgaaaa ccctgccgct gacacagatc agttacggta tcgtcgaagc
     2701 catcaagcac ggggcgatct gcgacccggc atactacaag ttcatctaca agaatcgagc
     2761 cgccatcaag aaccgcgacc tgctgctggt acagcgcctg gtgcgccgtt cgctgcacct
     2821 gaagaagaac gtcgtcgaac aggacgaaca ggatcagggg ctgcgccata cgctcaactt
     2881 cgggcacacc ttcggtcatg ccctggaaac cctgggcgag tatcgccggt atcatcacgg
     2941 cgaggctgtc ggcatcggca tgctgttggc cctgaaggcc gcgaaagccc tgcaccggct
     3001 gcaggaagac tattcagaaa gcctgcgcga gctgctgctc gatttcaacc tgccggtcga
     3061 cccgccccgc gaatggggcc ccgagcagtt taccaaggcc atcgcgcacg acaagaaggc
     3121 gctgaaagac aagatcaagt tcgtgctgcc gcttgcgctc ggcaaggttg aagtggtatc
     3181 agttccagcc gcggacgtcc ccggtctctt cgagcagatt ctcggctgat cgagactcgc
     3241 gcgcctcagg ttccccaagg agtttttcat gcctcattca cccgtatccg cctcactcag
     3301 cgggtttacc ccgaacgccc agaagattct cgccaagcgc tatctgacca agaacgaaga
     3361 cggcaccctg aaagagacga ttcccgaact gtttcaccgg gtcgcccgcg caattgccga
     3421 agctgaaagt cggttctcgc actcgccctt cagcgtcgag cagctggccg acaagttcta
     3481 tcagatgatg atcgcgcagg agttcatgcc caacagtccg accctgatga atgccggaaa
     3541 gcctctcggt cagctttctg cgtgtttcgt tctaccggtc ggcgattcgc tcgactcgat
     3601 cttcgactcg atcaagtttg ccgccatgat tcacaagtcg ggtggcggca ccgggttcag
     3661 cttcgcgcgc ctgcgaccgg ccaacgatat cgtcgccacg accaacggcg tcagctcagg
     3721 accggtctcg ttcatgcgcg tcttcaacat cgccaccgag acgatcaagc agggcggtac
     3781 tcgccgtggc gccaatatgg gcattctgcg cgtcgaccac ccggacatcc tggatttcat
     3841 caaatcgaag gaagccaacg actctctcaa caacttcaac atctcggtcg ccatcaccga
     3901 ccggttcatg gaagccctgg ccaaaggcga ggactacgag ctgttcaacc cgcgcaccaa
     3961 cgacgtggtg aagaagctga acgcccgcaa ggtgttcaat ctcatcgtcg aaggcgcctg
     4021 gcgcaacggt gagccgggca tcatcttcat cgaccgcatc aatcgcgaca accccacccc
     4081 gcaggtcgga ctgatcgaat cgaccaaccc ctgcggagag caaccgctgc tgccgtatga
     4141 atcgtgcaac ctcggctccc tgaacgttgc caagtttctc attgccgacg gggcgttctc
     4201 gcgtatcgat tacccgcgcc tgaaacaggc gattcacttg agcattcggt ttctcgacaa
     4261 cgtgatcgag gtcaaccggt accccctgcc cgatatcgag cagatgacca aagccaatcg
     4321 taagatcggt ctcggcatca tgggctggtc agacctgctg atcagactgc gcatctcgta
     4381 caactcggaa atggcactca ataccgcaga gcagctgatg agcttcattc agcgcgagtc
     4441 gtgggcggcc tcagcacagc tcgcccagga acgcggacac ttcgccaact tccccggttc
     4501 ggaactggaa aagcgcggca tgagcccagt gcgtaacgcg acggtcacga cgatcgcccc
     4561 cacgggcacg atcagcatga tcgccaactg ttcgagcggc gtcgaacccc tgttcgccat
     4621 cgcct
//
LOCUS       DAORHD010000207         4624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19683_length_4624_cov_3.503174, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000207 DAORHD010000000
VERSION     DAORHD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19683_length_4624_cov_3.503174"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1364)
                     /locus_tag="PKO06_05745"
     CDS             complement(27..1364)
                     /locus_tag="PKO06_05745"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV69182.1"
                     /translation="MKSSTADFRALPIQAPSRLFLMISLMAVLFSLFLFVNASRADEP
                     TGSPASASATGAQQATPVIAQKVTTSDIHRQLLTSGDILPLLGVDLFPKVSGQIVRFD
                     VSEGSLVKEGDLVAEIDHRIQDTQLEQAEAALTVAKAAVDMQEVMVKTAQSGLTAAKA
                     QADAVRAQVTNLVTSRTRLEKLHKEGAISRQQLDDVIAQHDAAQAQLVAASSTINQAE
                     DAIRSSQMTLKMKQAQLVQAQANLHAVQVQREDAFIRAPFSGVITRRNADPGAMANPG
                     QPLLRLEKMNQVKIVGTLVEKNLLLLTPERTEARVRVDTLDKEFTGTVAKIYPAIDPK
                     TRTGQFEVILENPELTLRSGMFTHIRLYLETVQGAVVIPRDALLSHNGEQAAIRISAN
                     GTAEKVSVRTGIVQDDRVEILDGLKAGDMIVTQGLEFVRVGSLVKAIVAEAKP"
     gene            complement(1378..2889)
                     /locus_tag="PKO06_05750"
     CDS             complement(1378..2889)
                     /locus_tag="PKO06_05750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69183.1"
                     /translation="MRLSSVVPLLIGIVCAFFSGSLAAEPASAPVGSTTPIEATTVSP
                     LPEVASDASQSDAEMASRTATTLSSTVAAPDSENATNSMGNLHLEEIQRLALKQNHSV
                     ISSRQQVEIAEGQVKQARSVSSTRLTGKMTQTRLDDVGAINIGGRTIAMGKKDTQRAY
                     VEMAQPLFLGGKDRATISAARLGRSAAQAGHFFTRQQVLRQATMSWLAWLFASEVEKV
                     ARKDLDLANEHHRLIEARLRQDQASKFELLRADVRLAQARSKYRQEQNNSDLARLELL
                     RVLALPADLMIGTQDRLSMQEFEPNPIRDASEAVFLREDLRIKRLEAEISRQGLRAAR
                     GEKQPTLNLFGQVGSEDPSSKSGLGGLQRKSYWNAGLALEVPLADGGQRRGKIQEATA
                     RLALAENALQEALEKTHIEIKQALLNLETAQEVVRSQEEALKQAEEALRLANVKYTNG
                     MFTQVEMFDAENAFLNTNLQYLQAVYAHHQARVAYLLATGQLGRNLLAPASAL"
     gene            complement(2902..3483)
                     /locus_tag="PKO06_05755"
     CDS             complement(2902..3483)
                     /locus_tag="PKO06_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV69184.1"
                     /translation="MARPPSDRRERILKAAITCFSTQAYPDVKLDQVAEQAGVAKGTI
                     YLYFRTKDDLFCQCLLDDAPHWISQTEKIIHSAETAAKRLHRMVVLQSKAYQLKGPLI
                     QSLLQMGPTFPLSTEIIARLHDHLRQVVARYASLFQDGLAAGEFSDRFTATQMAIIFI
                     QIFDLNVKFQMFQVPLLRPDDVYDALMKLFGPT"
     gene            complement(3749..>4624)
                     /locus_tag="PKO06_05760"
     CDS             complement(3749..>4624)
                     /locus_tag="PKO06_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69185.1"
                     /translation="QRGKTMADYMKNLADQTGSELKGITAGGEIQLFRDGTGKVADRL
                     GRQAADIGGAFDQTFTNSRTTSSQLEGIKAQIDSALQASGRSAGEVTLHDLQLSGAEL
                     SKKLIEARKYMVQSDKALVAADRSNSSLHVEIMGLLNGIKGDLETYAKMNGIDPDKLT
                     RTMQDPTLRKNLESATVPPIAADKAGTKKYQLTDEISFMVDKVDHMQQSSSRSITVSP
                     GAQTVNGATIAPLQNRESATTTSLHQQKVQAFKAYMETLAREPGNAKALETAQKAFEK
                     AEQQYRSSLEKDTQR"
BASE COUNT          897 a         1259 c         1370 g         1098 t
ORIGIN      
        1 ccaccgcacg ccgaaaatag aaagattcat ggcttggctt ctgctacgat ggccttgaca
       61 agacttccaa ctcggacaaa ctccaggccc tgggtcacga tcatgtctcc agccttcaat
      121 ccatcaagaa tttccactcg atcatcctgg acaattccgg ttcgcacgga aaccttctcg
      181 gcggtaccgt tggctgaaat tctgatcgcg gcttgttctc cgttgtgcga cagcaacgcg
      241 tcgcgcggaa tgacgaccgc gccctgtacg gtctcgagat acaggcggat atgcgtgaac
      301 atgcctgagc gcagagtcag ttcgggattc tcgagtatga cttcgaactg cccggtacgg
      361 gttttggggt cgatggccgg atagatcttg gcgacggtac cggtgaattc cttgtcgagc
      421 gtatcgactc gcacccgcgc ttctgtacgt tctggggtga gcaggagcag attcttctcg
      481 accagcgttc cgacgatttt gacctggttc attttctcca gacgcagaag tggttggccg
      541 ggatttgcca tggcaccagg atctgcgttt cggcgggtga tcaccccgct gaaaggagcg
      601 cgaatgaaag cgtcttctct ctgcacttga acggcatgaa gatttgcctg agcttgtaca
      661 agttgcgcct gtttcatttt cagtgtcatc tgactcgaac gaatggcatc ctcggcctga
      721 ttgatcgtcg aggaagcggc gaccagttgg gcctgagccg cgtcatgctg agcaatgaca
      781 tcgtcgagtt gctgcctcga aatggctcct tccttatgca gcttttcgag acgggttcgg
      841 ctggtgacca gatttgttac ctgtgcgcgc acggcatctg cctgagcttt ggcagccgtt
      901 aacccggact gagctgtctt caccatgacc tcctgcatgt ccacggcggc tttggcgacg
      961 gtcagcgccg cttcagcctg ttcgagctgg gtatcctgaa tgcgatggtc gatctccgcc
     1021 acgagatctc cttccttcac cagtgaccct tcgctgacat cgaaacgtac gatctggcca
     1081 ctgactttcg gaaacagatc aacgcccagc agcggcagaa tatcgccgga agtcagcagc
     1141 tgacgatgaa tgtcgctggt cgtgaccttc tgcgcgatca cgggcgttgc ttgctgcgcc
     1201 cccgttgctg aggccgatgc gggggagcct gtcggctcat cggctcgcga ggcgttgacg
     1261 aagagaaata gcgaaaacag taccgccatc aggctgatca tgagaaacag gcgcgagggc
     1321 gcctgaatgg gaagagcgcg aaagtctgct gtcgatgatt tcatgcgttg tctcctttca
     1381 cagagctgaa gcgggggcca gcagattgcg gccgagctga cccgtagcca gcaggtaggc
     1441 aacgcgagcc tggtggtgag cgtagactgc ctgcagatac tgaaggttcg tgttcagaaa
     1501 ggcattctcg gcgtcaaaca tttccacttg agtgaacatg ccgttggtgt atttcacgtt
     1561 ggccagacgc agagcctctt ctgcctgttt cagcgcctct tcctgcgagc ggacaacttc
     1621 ctgagctgtc tccaggttca acagggcctg cttgatctcg atgtgcgttt tttccagtgc
     1681 ttcctgcaag gcgttctcgg cgagtgccag acgcgcggtt gcctcctgaa tcttgcctcg
     1741 acgctgaccg ccatcggcca acgggacttc gagcgccagt ccggcgttcc agtagctctt
     1801 acgctgcaag ccgcccagac ctgatttcga agacggatct tcggacccga cctggccgaa
     1861 cagattcagt gtgggttgtt tctcgccgcg cgcggctcgc agaccttgac gactgatttc
     1921 cgcctccaga cgctttatgc gtagatcctc tcgcagaaaa accgcttctg atgcatctcg
     1981 aattggattg ggctcgaatt cctgcatcga gagacgatcc tgggtgccga tcatcaggtc
     2041 ggctggcagg gcgagcacac gcagaagttc gagccgcgca agatcgctgt tgttctgctc
     2101 ctggcgatac ttgctacgtg cctgagccag ccgaacatcc gcgcgcagaa gctcaaactt
     2161 cgaggcctga tcctgtctga gtcgtgcttc gatcaatcgg tgatgctcat tggccagatc
     2221 gagatccttg cgggccactt tctcgacctc gctggcgaac aaccaggcca gccaggacat
     2281 ggtggcctgg cgtaacacct gctgccgggt gaagaagtgg ccagcctgag cggccgatcg
     2341 tcccaggcgt gccgccgaaa tggtcgcgcg gtcttttccg cccaaaaaga gcggttgtgc
     2401 catttcgaca taggctctct gagtgtcttt cttgcccatg gcaatggtac gcccgccgat
     2461 attgattgcc ccgacatcat cgagacgggt ttgggtcatc tttccggtca gacgggttga
     2521 gctcacggaa cgggcttgtt tcacctgccc ttctgcaatc tcgacctgct gacgtgaact
     2581 gatgacgcta tgattctgtt tgagcgccag tcgttgaatc tcttccagat gcagattacc
     2641 catcgaattc gtcgcgttct cactgtcggg cgcggcgact gtcgacgaga gggtggtcgc
     2701 ggttctgctc gccatttcag cgtccgattg agacgcgtct gaggcaacct ccggaagtgg
     2761 cgaaacggtt gtcgcttcga tgggtgtcgt tgagccgacc ggtgccgatg cgggctcagc
     2821 tgcgagtgac ccggagaaaa acgcgcagac aatgccgatc aacagaggaa caactgagga
     2881 tagacgcatg agtggctctc cttaggtggg gccgaacagt ttcatgagcg catcgtagac
     2941 atcgtcaggt cgcagcaacg gaacctgaaa catctgaaat ttcacgttca ggtcgaaaat
     3001 ctggatgaaa attatagcca tctgtgtcgc ggtgaagcgg tcagaaaact cgccagcagc
     3061 caagccatcc tggaaaaggc tggcatacct ggccacgacc tggcgcagat ggtcgtgaag
     3121 ccgggcgatg atctctgtac tgaggggaaa cgtgggcccc atctgaagca gactttgaat
     3181 cagcggcccc ttgagctggt acgctttgct ttgcaatacc accattcgat gcaggcgctt
     3241 ggcagccgtt tcggcgctgt gaatgatctt ttcggtctgt gaaatccagt gcggggcatc
     3301 atcgagcagg cactggcaga acagatcatc tttggttctg aagtaaaggt agatggtccc
     3361 tttggcgacg cccgcctgct cggcaacctg atccaacttc acgtcgggat aggcttgagt
     3421 actgaagcag gtgatggcgg ctttgagaat gcgttcgcgt cgatcagaag gcgggcgggc
     3481 catgcggtgt ctcctgtgac gattgaaagt gaggatgacg ggtaacgtaa ttcatgactg
     3541 atcagtcagt catgaatata gcacagcacc tgtctgagcg caagaaaaac gcgtgagttg
     3601 agagtacgag tcgctggaac tgagacgcct gtagtgaaga ttgtgtcggc gagttgtatc
     3661 tcgcgcagtg ccgcgcgatt gcgatcagga caaaagaaaa ccggttgttc gcgtgcagcg
     3721 aacaaccggt tcgaaacggt ttccttgatc agcgttgggt gtctttttcc agggacgacc
     3781 gatactgctg ttccgctttt tcgaaggctt tctgagcggt ttcgagcgct ttggcattcc
     3841 cgggttcacg ggcgagtgtt tccatgtagg ctttgaatgc ctgcaccttc tgctgatgaa
     3901 gcgaggtggt cgtggcgctc tcgcggttct gcaacggggc gatggttgct ccgttcacgg
     3961 tctgtgctcc tggcgagacg gtgatggaac gcgaagagct ctgctgcatg tgatcaacct
     4021 tgtcgaccat gaagctgatc tcgtcggtca actggtactt cttggttccc gctttgtcag
     4081 cggcaatcgg cggcaccgtc gcggattcga gatttttgcg gagcgttgga tcctgcatgg
     4141 tgcgggtgag tttgtccggg tcgatcccgt tcatcttggc gtaggtttcc aggtcaccct
     4201 tgataccgtt cagcaggccc atgatctcga catgcagact ggagttgctg cgatcggcag
     4261 cgacgagtgc cttgtcagac tgaaccatgt atttgcgggc ttcgatcagc tttttgctga
     4321 gttcagctcc cgaaagctgc aggtcgtgca gggtgacttc acccgccgat cgaccacttg
     4381 cctgcagggc gctgtcgatc tgtgccttga tgccctcgag ctgagagctg gtggtgcggg
     4441 aattggtgaa cgtttgatcg aaagcaccgc cgatatcggc tgcctgacgg ccgagtcggt
     4501 cggccacttt accggtgcca tcccggaaca gctgaatttc tccgccggct gtgatgccct
     4561 tgagttcgct cccggtctgg tcagccaggt ttttcatgta gtcagccatg gttttaccgc
     4621 gttg
//
LOCUS       DAORHD010000208         4618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19733_length_4618_cov_3.330703, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000208 DAORHD010000000
VERSION     DAORHD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19733_length_4618_cov_3.330703"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3506
                     /locus_tag="PKO06_05765"
     CDS             <1..3506
                     /locus_tag="PKO06_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69186.1"
                     /translation="QILCLLLALTMGGTGPFRLDAAPVSVFSEAQIRHLPESEIAVIV
                     AARETGDDSESLPTRLRKTWSGVRPPKKSTGSEAAKISRATPTRPLTRPVREPRRVAS
                     ASARVKPMKPTTVAGQAVPLATETANNTASRSILAASSSRDLASTTAMTVLPGREPVS
                     SVPQVPVSDSDEMPVELKSDVASHAFVTPVVHAEPTHVEATVASAMPAAPLAPVPVAI
                     ASSPGAVSGTAPSQSQPAKAKDPFTAGGLTIDNGPIPENLGSVSYAIIEPEVVAEGAS
                     SAEVLPVPEINGTSSSQQKTATEAAVVPAIPAMVASATVNGPESHSLPPPLPPVESIE
                     PERGLNISGSKTFEMKNAKVKGDVGHFSTENYESIPGFRLDQSLHLEIEGNISRHTSV
                     NAVLDDKQDEDRRFTVFIDGSNWDLTLGDFPIALKNTEFLLHNKEVRGILAEGRVTSK
                     IKSTFLFSQSKGRFRREQFRGAGQQQEFRLLGRPVVQNSERIFIDGVALQRGSQYLID
                     YEDGILKLAPSQLPIEVTSWIVVEYEVADSKLAFKRNLYGMRIEDEIRSDRRLALTWL
                     REIDGSTPKSESNASGTVRPVDHQVIGLDGSWQIKPWLTANGELAFSLWDPNHLSELT
                     AEDKEVRDKAGRLNLSGVKGPLVAELALRRIGQNFKQIGREEGATVLGERGLVNDILK
                     QNGRLTYTLRPGLTLFTGLEDSRTNVSRDPTIPEVNFRSWTGGGTWKYRARSQFESRF
                     ESQRDEENRGTPYSNREKNVGTMVWDHDFGKIFTQTKLEHTAYEDAINVASDSRALVL
                     QSTISGPQRKGFAWTAGVGHIRLDDELDPDKVRSDTQNYSVDLTFDPSRMFNARGIFQ
                     WRRENDYFTRIQQDDQVADSRIRYQPFRDLNTQFKYKVENTTKVVRDPSIDPTKYVNP
                     PSLPVTKTDEEEVISRFENPVQKRTANFNAAYRFNQKAETNIDWKWRDLRDRTTKKQV
                     SYNDRKAYELKFAPMAKIQMTGGYEDGVMRNLAPVSELRDTLKRFEMRNEFKAGYILT
                     SRWEDHLEDDVLVETNDRRTEVRGAAFSRVFSARSTMELGLNRNIITSITPSREWEKK
                     AAFIFTPSSRNQRYKFFVTQRSIEAAKPGDYLEGGVNFSQFIGTDTMLDGELKRVTSS
                     ATVNGNGYEATVANAKMVITF"
BASE COUNT         1140 a         1198 c         1367 g          913 t
ORIGIN      
        1 gacaaattct ctgcctgctg ctggcgctga ccatgggcgg aacggggccg ttcagactgg
       61 atgcggctcc ggtatcggtt ttttccgagg cgcagattcg acatcttcct gagtcggaaa
      121 ttgccgtcat tgtggcggct cgtgagaccg gcgatgacag cgaaagcctg cccacccggc
      181 tgcgcaaaac ctggtcggga gtgaggccgc cgaagaagtc gaccggttcc gaagccgcga
      241 agatctctcg cgcgacacca acccggccgt tgaccaggcc ggttcgggaa ccacgacgcg
      301 tggcttcggc ttctgcgcgg gtcaagccga tgaaaccgac tactgtggca ggacaagccg
      361 ttcctctggc aacggagacc gcgaataaca cagcgtcgag atcgattctc gcggcttctt
      421 cgtcgcggga ccttgcttcg accacggcga tgaccgttct acctggtcgc gagccggtat
      481 cgagcgtccc acaagtaccg gtaagtgact cagatgaaat gcctgtggag ctgaaatcag
      541 acgttgcttc tcatgcgttt gtcactcccg tggttcatgc cgaaccgact catgtcgagg
      601 cgacagtcgc atcagcgatg ccagctgcgc ctctggcgcc ggttcccgtg gcgatagcgt
      661 cgagtccggg tgcggtatcg ggcaccgctc ccagtcagtc gcaacctgcg aaagcgaaag
      721 acccgtttac cgcgggcgga ctgaccatcg ataacggccc gatacccgaa aaccttgggt
      781 cagtcagtta tgcgatcatc gagcctgaag ttgtggcaga aggggcgtcg agtgctgaag
      841 ttctgccggt gcccgagatc aatggtacaa gttcatctca acaaaaaacg gcgacagagg
      901 ctgctgttgt gcctgcgatt ccggccatgg tggcttcggc tacggtcaac ggtcctgaat
      961 cgcactcgct gcctccgccg cttccgcccg tcgagtcgat agaaccagag cgcgggttga
     1021 acatctccgg gtcgaaaacc ttcgaaatga aaaatgccaa ggtgaaaggt gacgtcggac
     1081 acttttccac cgaaaactat gaatcgattc cggggttccg gctcgatcaa agcctgcacc
     1141 tggaaatcga aggcaacatc agtcgccaca ccagcgtcaa tgcggtgctc gatgacaagc
     1201 aggatgaaga tcgtcgattc acggtgttta tcgatggatc gaactgggat ctgacgctcg
     1261 gtgactttcc gattgcgctc aagaataccg agtttctgct gcacaacaag gaagtacgcg
     1321 gcattcttgc cgaagggcgc gtgacgtcga agatcaagag cacatttctc ttctcacaat
     1381 cgaaagggcg attcagacgt gagcagttcc gtggcgctgg acaacagcag gaatttcgtc
     1441 tgcttggtcg gcccgtggtg cagaactctg aacgcatctt catcgacggg gttgctctgc
     1501 agcgtggttc acagtatctg atcgattacg aagacggcat tctgaaactt gccccctctc
     1561 aactgccgat cgaagtcacg agctggatcg tcgtggagta cgaggtcgcg gactcgaaac
     1621 tggccttcaa acgcaatttg tatggcatgc gcattgaaga cgagattcgc agtgatcgcc
     1681 gcctggccct gacctggctg cgcgaaatcg acggctcgac ccccaagagc gaaagcaacg
     1741 ccagcggaac ggttcgaccg gtcgatcatc aggtgatcgg actcgacggc tcctggcaga
     1801 tcaaaccgtg gctcaccgcg aacggtgaac tggcgttttc cctgtgggac ccaaaccatc
     1861 tcagcgagct gaccgctgaa gacaaagaag ttcgtgacaa agccggacgc ttgaatcttt
     1921 cgggcgtcaa aggaccattg gttgctgaac tggccttgcg gcgaatcgga caaaacttca
     1981 agcagatcgg ccgtgaagaa ggcgcgaccg tgctgggcga acgcggtctg gtgaatgaca
     2041 ttctgaaaca gaacggaagg ctgacctata ccttgcgccc gggactgaca ctgttcacgg
     2101 gtctcgaaga ttcgcggacg aacgtgagtc gcgatccgac cattcctgag gtgaactttc
     2161 gcagttggac tggaggggga acctggaagt atcgggctcg ctcacagttc gagagtcgct
     2221 ttgaaagcca gcgtgacgag gaaaatcgcg gtactccgta ttccaatcgg gaaaagaacg
     2281 tgggcaccat ggtctgggat cacgatttcg gcaagatctt cacccagacg aaactcgaac
     2341 acacagctta tgaagacgcg atcaatgtgg cttctgattc aagagcactg gtactgcaga
     2401 gcactatcag cggtccgcaa cgcaaagggt ttgcctggac agcgggagtt gggcacatcc
     2461 gacttgatga tgaactcgat ccggacaagg tgcgctcgga cacacagaac tacagtgtcg
     2521 atctgacctt tgaccccagt cgtatgttca acgcccgcgg cattttccag tggcgtcgcg
     2581 agaatgacta tttcacccgg attcaacagg atgatcaggt cgctgactcg cgcattcgct
     2641 accagccgtt tcgcgatctg aacacccagt tcaaatacaa ggttgagaac acgaccaaag
     2701 tcgtgcgcga tccgagcatc gacccgacga agtatgtgaa tcctcccagt ctgccggtga
     2761 cgaagaccga tgaagaggaa gtgatcagtc ggttcgaaaa cccggtgcag aagcgcacgg
     2821 caaacttcaa tgccgcctat cgcttcaatc agaaggcaga gacgaacatc gactggaagt
     2881 ggcgtgatct gcgcgatcga accaccaaga aacaggtttc ctacaacgac cgcaaagcct
     2941 atgagttgaa gtttgcgccc atggccaaga ttcagatgac ggggggctat gaagacgggg
     3001 tgatgcggaa tctggcgccc gtcagtgaat tgcgcgatac cttgaagcgt tttgagatgc
     3061 gcaacgagtt caaggccggt tacatcctga ccagccgatg ggaagaccac ctggaagatg
     3121 atgttctggt cgagaccaat gatcggcgca ccgaagttcg cggagctgcg ttcagtcgcg
     3181 tgttttcggc acgatcgacc atggagctgg gtttgaatcg caacatcatc acttcgatca
     3241 ccccgagtcg agagtgggaa aagaaggcgg cgtttatctt caccccttcg tcgaggaatc
     3301 agcggtacaa gttcttcgtg acgcaacggt cgatcgaagc tgcgaagcct ggtgactatc
     3361 tcgaaggcgg ggtgaacttt tcccaattca tcggtaccga caccatgctt gatggtgagt
     3421 taaagcgggt gacgtcatcg gcaaccgtca atggcaacgg atacgaggcg accgtggcta
     3481 atgccaagat ggtgatcacc ttttaaaaag aatttgaaaa aaagtgaaaa tacctcttga
     3541 cttcgacctt gattatccgt atattggatt ctgttgagtg accgatcttt gaaaagcgaa
     3601 ctggaagccg caggaagaca tcttcggaac ccctaaccgg gtcgagtccg gacaggcggt
     3661 gctgcattag gttgttcggg attgctggtg acagtggttc ggacctaaag gaaatgcata
     3721 acctaagcat cgcgcgtgct ttaagatgaa gatgccttca gaaggcaaaa acctccagag
     3781 ggaaggtagt cgcaagaccg ccatcttctg cagtagaaca aaccctgtag gggccgcaag
     3841 gaaccccaag agggaggagt gccctgagca cctggaccgc agaggaagaa agccctgaag
     3901 aggcgggaag gccaagagag ttgccactgt ctgcatcggg tgaaactgat gtaggtacag
     3961 ggcgcgtcgg agcgggaaga aaccgttcga gtcggccgaa gtccaagatg gtagccggtt
     4021 ccccacaaag gaacgagggg ccatgaagcg ggtgcagtcg tcggaagtag agttctgatg
     4081 gccagaacgg cgtggaaccg tcacctgagt cctggaagga aaccatgaag gataggtaac
     4141 gtcagaagcg gatcagaatc ggataacgat tacggatcgc tccgaagtta tactctgaag
     4201 gtcgtaaccc tgggagtgcc agtagggtgg aataaaccta caagctgtat cgcgacgaaa
     4261 agacctgttg aaaggttgta aaaacctacg aacggggagc gtcagaaccg gaagttctgg
     4321 tcgtctcgtc gggcagtgat ggcgctgaga gggaaaaaac ccaaggcgac accggggtcc
     4381 tgaccggtaa tgcgcgtggt aataccctga agtcggctaa agctcacaag aggacagtta
     4441 ctctgtggaa ctcccacaaa gtggcagtcg attcgatggc ctcgagggcg ctgtgaacgc
     4501 ctgaggcttg gtgtaaccaa taggcaccat agatcgattg tgttaaaact ctagagagca
     4561 ggtaacgaca cgagggttgg ttccgagtcg agagactcga aatcagctag gcgctgac
//
LOCUS       DAORHD010000209         4616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19745_length_4616_cov_4.279763, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000209 DAORHD010000000
VERSION     DAORHD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19745_length_4616_cov_4.279763"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="PKO06_05770"
     CDS             <1..791
                     /locus_tag="PKO06_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005510977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV69187.1"
                     /translation="PTDKVQAIRDYQQVDGAAQVAKPVVVMVGDGINDAPALAQADLG
                     IAIGTGTDVAMASAGVTLIGGDLHGIGRAVALSRRVYRTVTENLVWAFFYNVALIPIA
                     ALGLLVPMVAAGAMTFSSLFVVTNSLRLRHWQPGQPTEPPRDLSGRLLEALPRLITPI
                     ATLAVLIVLPMLIMPNGMEIQAVRAEAMDPILMMVMAIANGLIAVSYGSIPVFLLVFV
                     QKRKDIPFFMGLRHVRPVHSRLRSHPRHPHHRPVVESGLVASLL"
     gene            697..2556
                     /locus_tag="PKO06_05775"
     CDS             697..2556
                     /locus_tag="PKO06_05775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69188.1"
                     /translation="MFGLFILACGATHVTHIIGLWWKVDWWQAFFDSACAFISLATAI
                     LLWPLLPKFLAIPSPEQLRQVNRELQREKEKLEQAQADLQKSYAEVEHRVRERTRELA
                     TANDALQSEIAVRKQAEAELQRNRDHLEELVKERTRELEAATQAAEAANQAKSMFLAN
                     MSHELRTPLNAIIGFSDILVRMINDPKQSHYITRIRTGGNTLLQLINDILDLSKIEAG
                     KMVLNIRAVSVKQLIEESCEMFSHKLSEKSLEIVREIPADFPASVLLDGPRLRQILMN
                     LIGNAVKFTDDGTITVKAWSISHVDGPDSSPDLYISVQDSGIGIAPDHLTKLFLPFEQ
                     QKSTIARGIGGTGLGLAITRKLVEAMGGEISVTSEVGKGSTFTVKFREVEISAGFADV
                     PEAVVDTDGFDYEGVTFERARILVVDDIEYNRDLIRSFFSGYNFDLIEAGNGLEALEK
                     ARELHPHLVLLDMKMPIMDGYEASTHFKQDPELSKIPVIAVTASALKSDEVEIKKTCN
                     AYLRKPLRRYDLIRVTMDFLPHTQRGVAKGDAATATALTTDNFSQRVANLPATLVQRL
                     IAAAPLADMLEIQQALEEIQTLDPALAQSLRRFASRFDYDGLVQALKGQEEQP"
     gene            2553..4568
                     /locus_tag="PKO06_05780"
     CDS             2553..4568
                     /locus_tag="PKO06_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69189.1"
                     /translation="MTDNPASILLVDDVVDNLQVLSDILMREGFRVRAVTSGAMALKT
                     VESAVPDLFLLDIKMPQMDGYELCRRLKADEKMRDIPVIFVSAMGETTDKVRGFEVGA
                     VDFVTKPFQASEIVARVRTHLTLVTMRRTLHDLNQHLEQRVEERTRELAQTNLRLAQE
                     VAERRQAEADRDRKTEEINQLFNMSIDLLCIADVEGRFVRLNSTWEKTLGYTLSELEG
                     KRYIEFVHPDDVAATLEAMAQLAAGKPIIDFSNRYRCRDGSFRLIEWRSKPYQNTFVY
                     AAARDITERNQMQEALKKSEQQLQQMQKMEAIGQLAGGIAHDFNNQLGGIIGFVDLLQ
                     LDLEKPEHRTYCNKILEITKRAADLTSKLLAFARKGKFLSAPVDIHALVPQIVEILQR
                     SIDKRISIRQELSSPVSVTLGDQTQLQNALLNLAINARDAMPNGGELSFATALVDLNE
                     RAAQMGIAPGRYLEIRVSDTGSGMSPETQKHVFEPFFTTKPAGHGTGLGLASVFGTVK
                     NHRGGILFYSELGHGTSFKIYLPVSSKEVQPEAEKHSEPIKGNARILIVDDEEGIRES
                     LALILQKLGHTVTSCCDGEEALSLYASRWQQFDLVILDMIMPRMSGGDVFEKMHAINP
                     QVCVLLSSGYSLNGQAQSILDRGVKGFLHKPFQANELSQVIARVLDH"
BASE COUNT         1023 a         1366 c         1295 g          932 t
ORIGIN      
        1 caccgaccga caaggtgcag gcgattcgcg attaccagca ggtggacggg gcagctcagg
       61 tcgcgaagcc agtcgtcgtc atggtcggcg atggcatcaa cgacgccccg gctctggcac
      121 aggccgacct cggcatcgcc atcggtaccg gcaccgacgt cgccatggct tcggccggtg
      181 tcaccctcat tggtggcgat ctacacggca tcggccgcgc cgtcgcgctg tcgcgccggg
      241 tgtatcgcac ggtcacggaa aacctggtct gggcattctt ctacaacgtc gccctgattc
      301 cgattgccgc gctgggcctg ctggttccga tggtcgcggc cggcgccatg acgttcagtt
      361 cgttgtttgt cgtcaccaac agtcttcgcc tgcgccactg gcagccgggc cagccgaccg
      421 agcccccgcg cgatctttcc ggtcgactgc tcgaagccct gccgcgattg attacgccga
      481 tcgccacgct cgccgtgttg atcgttctgc cgatgctgat catgccgaac gggatggaga
      541 ttcaggccgt gcgtgccgag gccatggatc cgattctgat gatggtcatg gccatagcga
      601 acggtctgat cgcggtttcc tatggttcaa tcccggtctt tctgctggtg ttcgttcaga
      661 aacgaaaaga catccctttt ttcatgggtc ttcgtcatgt tcggcctgtt cattctcgcc
      721 tgcggagcca cccacgtcac ccacatcatc ggcctgtggt ggaaagtgga ctggtggcaa
      781 gccttctttg attcagcctg cgcctttatc tcgctcgcca cggcaattct gctctggccg
      841 cttctgccga agtttctggc gattccgagc cctgaacagc tcaggcaggt caatcgcgaa
      901 ctgcagcgtg aaaaggaaaa gctcgaacag gctcaggccg acttgcagaa atcttatgcc
      961 gaggtcgaac accgggttcg tgagcgcacc cgtgaactcg caaccgcgaa cgatgctctg
     1021 caaagtgaga tcgccgtacg caaacaggcc gaagctgaac ttcagcgtaa tcgagatcat
     1081 cttgaggaac tggtcaagga gcgtacccgc gagctcgagg cagcaaccca ggcggctgag
     1141 gccgcaaacc aggccaagag tatgtttctg gccaacatga gtcatgagct gcgcaccccc
     1201 ttgaacgcca ttatcggatt ttctgacatt ctggtgcgca tgatcaacga cccgaagcag
     1261 tcgcactaca tcacccgcat tcgcaccggc ggaaacacgc tgctgcagtt gatcaacgat
     1321 attctcgacc tttccaagat cgaggcgggc aagatggtct tgaacattcg tgccgtgtcg
     1381 gtgaagcagc tgatcgaaga atcgtgcgaa atgttcagtc acaaactttc ggaaaaaagt
     1441 ctcgaaattg tccgagagat tccggcggac tttccggctt cggtgctgct cgacgggcca
     1501 cgccttcgtc aaattctgat gaatctcatc ggcaacgcgg tcaagttcac cgacgatggc
     1561 accatcacgg tgaaagcctg gagcatcagc cacgtggacg gcccggacag cagccccgac
     1621 ctgtatatct cggttcagga ttcaggcatc ggcatcgcgc ccgatcatct caccaagctt
     1681 tttctgccgt ttgagcagca gaagtcgacg atcgcacgtg gtatcggcgg aaccggtctc
     1741 ggcctggcca tcacccgcaa gctggtcgag gccatgggcg gcgagatcag cgtcaccagc
     1801 gaagtcggaa aaggcagcac gtttacggtc aagtttcgcg aagttgagat ctcggccgga
     1861 ttcgcggatg tacccgaggc agtggttgat accgatggct tcgattacga gggcgtcacc
     1921 ttcgagcgtg cccgtattct ggtggtcgac gacattgaat acaatcgcga tctgattcgc
     1981 agtttcttca gcggctacaa ttttgatctg atcgaagcag gcaatggcct cgaagctctc
     2041 gagaaagcgc gcgaactgca cccgcatctg gtgttgctcg acatgaagat gccgatcatg
     2101 gacgggtatg aagcatcgac tcacttcaag caggatcctg agctcagcaa gattccggtg
     2161 atcgctgtca ccgcgtcggc gctgaagagc gacgaagtcg aaatcaagaa gacctgtaac
     2221 gcctacctgc gaaagccgct caggcgctac gatctgattc gtgtgaccat ggactttctc
     2281 ccgcacacgc aacgcggcgt agcgaaaggg gatgccgcaa ccgccacagc actgacgaca
     2341 gacaactttt cgcaacgcgt ggcaaacctg ccggcaacgc tggtccagcg gctgatcgcc
     2401 gcagctccgc ttgcggacat gctggagatt caacaggcgc tcgaggaaat acaaaccctt
     2461 gaccctgcgt tggcgcaaag tctcagacgt ttcgccagtc gtttcgatta tgacggactg
     2521 gtacaagccc tgaaaggtca ggaggaacaa ccatgacgga caatcccgcc agcattctgc
     2581 tggtcgatga tgtggtcgat aacctgcagg tgctttccga cattctcatg cgcgaaggat
     2641 ttcgcgtgcg cgcggtcacc agcggcgcca tggcgctgaa aaccgtcgag tcggcggttc
     2701 ctgacctgtt tctgctcgac atcaagatgc cacagatgga cggctatgaa ctctgtcgcc
     2761 ggctgaaggc cgacgaaaaa atgcgtgaca ttccggtcat tttcgtaagt gccatgggag
     2821 agacgacgga taaggttcgc ggattcgaag tcggagccgt tgacttcgtc accaagccgt
     2881 ttcaggcgag cgagatcgtc gcgcgggtca gaacccatct gaccctggtc accatgcgac
     2941 gcacactgca cgatctgaac cagcacctcg agcagcgagt cgaggaacgc actcgtgaac
     3001 tggcacaaac caaccttcgc ctcgcacagg aggtcgcaga gcgcagacag gccgaggccg
     3061 accgggacag aaaaacggaa gagatcaacc agctgttcaa tatgtccatc gacctgctct
     3121 gtatcgcgga tgttgaggga cgcttcgttc gcctgaactc cacctgggaa aagactctcg
     3181 gctacactct ctctgaactt gaagggaagc gctacatcga gtttgtgcac ccggacgatg
     3241 tcgcggccac actcgaggcc atggctcagc tagctgcagg aaagccgatt atcgactttt
     3301 ccaaccggta ccgctgccgc gacggcagtt tccgtctgat cgagtggcgt tcgaaaccct
     3361 accagaacac gttcgtctac gcagccgccc gtgacattac cgagcggaac cagatgcagg
     3421 aggccctgaa aaagagcgag caacagctgc agcagatgca gaagatggaa gccatcgggc
     3481 aactcgccgg aggtatcgcg cacgacttca ataaccagct cggcggcatc atcggtttcg
     3541 tcgacctgct gcaactcgat ctggaaaagc cggagcaccg cacctactgc aacaagattc
     3601 tcgagatcac caaacgagcg gctgatctga cttcgaaact tctggcgttc gcacggaagg
     3661 gcaagtttct ttcggccccc gtggacattc atgcgctggt gccacagatc gtcgagattc
     3721 tgcaacgcag catcgacaaa cgcatcagca ttcgacagga gctgagctca ccggtgtcgg
     3781 tgactctcgg tgatcagaca cagctgcaga acgctctgct gaacctcgcg atcaatgccc
     3841 gcgacgccat gccaaacggc ggagaactct cattcgcgac tgctctcgtc gatctgaatg
     3901 agcgcgctgc tcaaatgggt attgcgccgg gtcgatatct ggagattcgg gtttcagaca
     3961 ccggctccgg catgagccct gaaacccaga agcacgtgtt cgagcccttt ttcacgacca
     4021 aacccgccgg gcacggcacc ggcctcggac tcgcatcggt tttcggcacc gtgaaaaacc
     4081 atcgcggcgg gattttgttc tacagcgaac tcggacatgg cacatcattc aagatctacc
     4141 ttcccgtgag ttccaaggaa gtgcagccgg aagccgagaa gcattcagaa ccaatcaagg
     4201 gcaacgcacg cattctcatt gtcgatgatg aagagggtat tcgcgagtcg ctcgcgctga
     4261 ttctacagaa actcggtcac acggtcacct cgtgctgcga cggcgaagaa gctttgagtc
     4321 tgtatgccag ccgctggcag caattcgatc tggtcattct cgacatgatc atgccccgca
     4381 tgagcggagg cgatgtcttc gagaagatgc acgcgatcaa tccccaggtc tgcgtgcttc
     4441 tgtcatcggg atacagtctg aacggtcaag cccagagcat actcgaccgc ggggtcaaag
     4501 gctttctgca caagccgttt caggccaatg aattgtccca ggtcattgcc agagttctcg
     4561 accattgatg cttctgaccg acaggcatct tcacaaatta ccccgcctca ggcacg
//
LOCUS       DAORHD010000210         4612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19773_length_4612_cov_3.585253, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000210 DAORHD010000000
VERSION     DAORHD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19773_length_4612_cov_3.585253"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKO06_05785"
     CDS             <1..710
                     /locus_tag="PKO06_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV69190.1"
                     /translation="VDLLMIQKKIHRGVFAVMDGTFAGDGPGPRCMIPHVKNVILASA
                     DQVAIDAVAAKLMGFDPLRDIKFIRLAHEKGLGCGDPSQIEIVGDLEAARMNWQFQGP
                     FKEMTFAARQQHAIYWGPLKKPIEWSLKTWLAPWSYVASVFYHDWLWYPLFGKLPLKK
                     AMRSNWGRLFEHWSASQLNATGTGYSLPTTAEPGKTRITASHYLEAARLLLMAIRESP
                     EWQTRQRRQERESQRSR"
     gene            889..1830
                     /locus_tag="PKO06_05790"
     CDS             889..1830
                     /locus_tag="PKO06_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNV69191.1"
                     /translation="MLFNLFLSIVGLVILTIAADKLVEGAAGVAHRIGISPLVVGLTI
                     VAMGTSAPELVVSVQSSFEGNPGIAVGNCIGSNIFNIAAILGIAAIIRPIAVSRPVIR
                     RDVPIMIVTAGMAWLYAYDRQFSRFESVFMLLCMISYIAWSIIEARKNPEAAPPEETP
                     ALQTTLPGEILRIVLGLVALVGGSKLLLVGSVGLAKLAGISDEVIGLTLIAAGTSMPE
                     LATSVVASFRGQPDIAVGNVVGSNIFNILGILGVAGTLLPLDVSSHMFGIDLPLMVIV
                     SIACIPIMRSGMVISRLEGALLITGYTVYTVALVMGY"
     gene            complement(1921..2814)
                     /locus_tag="PKO06_05795"
     CDS             complement(1921..2814)
                     /locus_tag="PKO06_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69192.1"
                     /translation="MKRSRCGYVILGLVLGLLVGQGASLWAEDALETLFKLSGTTKLV
                     GEIPGLVSMGMQMAQQEKPIDPKELAELQKVAEEKYAADRFMKILREHIEKKLDQEKV
                     NKLVSWYNGELAQRVIKAEEESAKPDKILEMQAYVTMLAQKPLPEPRLALLKRLDAAI
                     RGSAINENMLLKVRMAMVRAMNAIQPEAKRQNEEQLKAVEERLVGEVQQVVEQQLIGS
                     WAYSYRDITDKDFETYVAFYETELGREWGISLGEALSVGMEQAGNELAKHYAEHLQKA
                     TEEAPQPAVASPVAPVASETR"
     gene            3033..4223
                     /locus_tag="PKO06_05800"
     CDS             3033..4223
                     /locus_tag="PKO06_05800"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002519851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNV69193.1"
                     /translation="MARNAVQCIGILTSGGDCPGLNAAIRGVAKAALKQYGMKVIGIQ
                     DGFRGLVENRTVNLDSDEVSGILTHGGTILGTARDKPHKMPMGGRELDMTSTAVENAR
                     RLGIDCLVCLGGGGTAKNALHLHKQGGIRVLTLPKTIDNDLVETDVTFGFDTGMNIAT
                     EAIDRLHTTASSHQRIIVCEVMGHNAGWLALGSGIAGGADVILIPEIPYDLDVVSRYL
                     LERRQSGKKFSIIAVAEGAISREEAERSARTNASDAVHGKKDKKNKKKQEPAVRAAED
                     DTAADEALARKHLMVQEPMASRIARQLQQMTGIEARVTSLGHVQRGGTPTPTDRLLCT
                     RLGTRAAELIAEGVFNVMVAVKGDRCEPVPLESVAGRKKLVPLDHPWVTSARLVGTCF
                     GNDW"
     gene            4232..>4612
                     /locus_tag="PKO06_05805"
     CDS             4232..>4612
                     /locus_tag="PKO06_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69194.1"
                     /translation="MEWLQAIILFVASVIGVLLALLLALVLIVSPVVMACRNLWSLYR
                     SSRRTIVKHDLFGDMEVYDQDALVKITLGGQELRVELPASAGAPAPAALETLVRFTQE
                     YESLESVIGIKAKDEWVIISESYDS"
BASE COUNT          905 a         1379 c         1296 g         1032 t
ORIGIN      
        1 tggtcgattt gttgatgatt caaaagaaga ttcatcgcgg ggtcttcgca gtcatggatg
       61 ggacatttgc cggtgacggc cccggcccac gctgcatgat tccgcacgta aagaatgtga
      121 ttctcgcttc ggccgatcag gtggccatcg atgccgtggc cgcgaagctg atggggttcg
      181 accccttgcg cgacattaag ttcatccgcc tggcgcacga gaaggggctc ggctgcggcg
      241 acccgtcgca gattgagatc gtcggcgacc tcgaggccgc ccgcatgaac tggcagtttc
      301 agggcccatt caaggaaatg accttcgccg cccgccagca gcacgccatc tactggggtc
      361 cgctgaagaa gcccattgaa tggtcgctca aaacctggct ggcaccctgg tcgtatgtcg
      421 cttcggtgtt ctaccacgac tggctctggt atccgctgtt cggcaagctg ccgctgaaaa
      481 aggccatgcg cagcaactgg ggtcgactat tcgagcactg gagcgcttct caactcaacg
      541 caaccggtac cgggtattcc ctgccgacca cggcagaacc aggcaagacc cgtatcaccg
      601 cctcacacta cctggaagcc gcccgtctgc tgctgatggc cattcgtgaa tcccccgagt
      661 ggcagaccag gcagcgtcgc caggagcgcg agtcacaacg cagtcgctga cccagagaag
      721 gtcagacttc atggcgccag ccacgcccct ttgagcgcgt gagggggcaa cggctctgca
      781 tactgaccgt ctgacagggc gcagacaaac cttcacttga gccagagggc ttctgcgctg
      841 tgctttgggg cgcttgcgtt tcggggaaaa cgtggtaaag taccggccat gttattcaac
      901 ctttttcttt cgatcgtagg tttggtaatt ctgaccattg ccgccgacaa gctcgtggaa
      961 ggcgcggccg gtgtggcgca tcgcatcggg atctcgcctc tcgtcgtcgg gctgaccatc
     1021 gtggccatgg ggacctcggc tcctgagctg gtcgtcagtg tccaatcgag ctttgaaggg
     1081 aacccgggaa tcgccgtcgg caactgtatc gggtcaaaca ttttcaatat tgccgccatt
     1141 ctcggcattg cggccatcat tcggcccatc gccgtcagtc gcccggtcat tcgccgtgat
     1201 gttcctatca tgatcgttac cgccggcatg gcctggctgt acgcgtatga ccggcagttt
     1261 tcccgtttcg agagtgtgtt catgcttctg tgcatgatct cttacattgc ctggagtatt
     1321 atcgaagctc gaaagaaccc tgaagccgct cctcccgaag aaaccccagc gcttcaaacc
     1381 acgttgccag gggagattct tcgcattgtc ctcgggcttg tggctctcgt gggcggatcg
     1441 aaactgctgc tggtaggttc ggtcgggtta gccaagctgg caggcatctc ggatgaagtc
     1501 atcggcctga ccctgatcgc tgccgggacc tcgatgcccg aactggcaac ctcggtcgtc
     1561 gcctcgtttc gtggccaacc agacatcgcc gtcggcaacg tcgtcggctc gaacatcttc
     1621 aatattctcg gcattctcgg agtcgctggc accctattgc cactcgatgt ctcaagccac
     1681 atgttcggca tcgatcttcc gctgatggtg atcgtttcta ttgcctgcat ccccatcatg
     1741 cgttcaggca tggtcatttc acgtcttgag ggtgcactgc tcattactgg atacaccgtc
     1801 tatacggttg ctctcgttat gggctactag gacaacaaga ttctcttcgt cgagaatcag
     1861 aaaaccgccc cgattgttct cgcgatcggg gcggttttct gtttcagcgt ttgcgaacgg
     1921 ttacctggtc tcgctcgcca ccggtgcgac tggcgaagcc actgccggtt ggggtgcctc
     1981 ttccgtggcc ttctgcaggt gttcggcata gtgtttggcc agctcattcc cggcctgttc
     2041 catcccgacg ctgagagctt cacccaggct gatgccccac tcccggccaa gctcggtttc
     2101 gtagaaggcg acgtaggttt caaaatcctt gtcggtaata tcgcgatagc tgtaagccca
     2161 tgatccgatg agttgctgtt cgaccacctg ctgaacttcg ccgacgaggc gttcttcaac
     2221 cgccttcagt tgttcttcgt tctgacgttt cgcctcgggc tgaatcgcat tcatggcacg
     2281 caccatcgcc atacgcacct tcaagagcat gttctcgttg atcgcgctgc cgcgaatggc
     2341 tgcgtcgaga cgcttcaaca gagccagacg aggttcaggc agtggtttct gagccagcat
     2401 ggtgacgtag gcctgcattt ccagaatctt gtctggcttc gcgctctcct cttccgcctt
     2461 gatcacccgc tgagccaact cgccgttgta ccagctgaca agcttgttca ccttttcctg
     2521 atcgagtttt ttctcaatgt gttcgcgcag aattttcatg aatcgatcag ccgcgtactt
     2581 ttcttccgcc accttttgca gctcggcaag ctctttcgga tcaatcggtt tttcctgttg
     2641 agccatctgc atgcccatac tcaccaggcc cggaatctca cccacgagtt tggtcgtgcc
     2701 cgaaagtttg aacagggttt ccagagcatc ttcggcccac agactcgcgc cttgccccac
     2761 caacaaaccc agcaccaacc ccaggatcac gtatccgcat cgcgaccgtt tcatcttccc
     2821 gcctcctgca acagaattcc tatctgaact gactgactca tgctactctc accgcgctct
     2881 cccgcgcaat ctctccaagg cactggcagg gtcatttcat tctcctacat acgtgttttc
     2941 cgttcattcg cgcggcggcg gttcttgtgt tactgtgcat cagctcccgc gtcgctcgaa
     3001 acgagaaggg cgcggaatcg gaaggagacc acatggccag aaacgcagtg cagtgcatcg
     3061 gaattctgac ttcgggcggt gactgtccgg gattgaacgc cgccattcgg ggtgtcgcca
     3121 aagccgctct gaaacagtat ggcatgaagg tcatcgggat tcaggacggc tttcgtggtc
     3181 tggtcgagaa ccgcacggtg aaccttgact cagacgaagt cagcggcatt ctgacgcatg
     3241 gcggaaccat tctcggcacc gcgcgcgaca agccgcacaa gatgccgatg ggtggtcgcg
     3301 agctcgatat gacatcaacg gcggtcgaaa acgcacgccg tctcggaatc gactgcctgg
     3361 tctgtctggg cggcggcggt acggccaaga atgcgctgca cctgcataag caaggcggga
     3421 tcagggtgct cacgctgccc aagacgatcg ataatgatct cgtcgaaacc gatgtcacct
     3481 ttgggttcga tacgggaatg aacatcgcga ccgaagccat cgatcgactg cacaccaccg
     3541 cttcgagcca ccagcgcatc atcgtctgcg aggtgatggg gcacaatgcc ggttggctgg
     3601 ccctgggcag cggtattgcc ggcggtgccg atgtcattct gattcctgag atcccgtatg
     3661 atctcgacgt cgtatcccgg tatctgctcg aacgacgtca gagcggcaag aagttttcaa
     3721 ttatcgccgt ggctgaaggg gcgatctcac gcgaggaagc ggaacgttcg gctcggacaa
     3781 acgccagcga tgcggtacac ggaaaaaagg acaagaagaa caaaaagaag caggaaccag
     3841 cggttcgggc agctgaagac gatacggccg ctgacgaagc cctcgcccgc aagcacctga
     3901 tggtgcagga acccatggcc agtcgtattg cgcgccaact gcagcagatg accggcatcg
     3961 aagcgcgtgt aacctctctg ggccatgtac agcgcggggg aactccgacg ccgaccgatc
     4021 gtctgctgtg caccaggctc ggcacccgcg cggccgaact gattgccgaa ggggttttca
     4081 atgtcatggt cgcagtcaaa ggagaccgct gcgaaccggt tccactcgag agcgtcgccg
     4141 gacgcaagaa actcgttccc cttgatcacc cgtgggtgac gagcgcgcgg ctggtcggaa
     4201 cctgcttcgg caacgactgg taagcccgac gatggagtgg ctgcaagcga tcattctgtt
     4261 cgtcgcctcg gtcatcggcg tgctgctggc tctgctgctg gcactcgttc ttatcgtcag
     4321 tccggtggtg atggcttgtc gtaacctgtg gagcctgtac cgatcatccc gacgcacgat
     4381 cgtgaaacat gacctgttcg gcgacatgga ggtctacgat caggacgctc tggtgaaaat
     4441 cacgcttggc ggccaggaac tccgggtcga gttgcccgcg tccgccggtg cacctgcacc
     4501 tgccgccctg gaaacgctcg ttcgtttcac gcaagagtac gaatcactag aatcggtaat
     4561 aggcataaaa gccaaggatg agtgggtaat tatatccgaa tcctacgact ct
//
LOCUS       DAORHD010000211         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19846_length_4600_cov_3.486909, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000211 DAORHD010000000
VERSION     DAORHD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19846_length_4600_cov_3.486909"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKO06_05810"
     CDS             complement(<1..387)
                     /locus_tag="PKO06_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69195.1"
                     /translation="MAYRRGWVMVLAVVWLGLNLVPACSSQRPTRTLTAEPEGGDSQV
                     PQSKLVELVKVTRVKANLRGIARHVDGSIWAVGYDGVIVRLDANLNTVATIPSGVKVP
                     LFDISFIDEKTGFIVGGSAQSMDDPKK"
     gene            577..1914
                     /locus_tag="PKO06_05815"
     CDS             577..1914
                     /locus_tag="PKO06_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69196.1"
                     /translation="MKRLALAVTLSSFFLFHGTQTPTLWAGAYDQLIDIGGPPPDVPM
                     PTNPTPVDPSPDNSNSQDNSSTSSYNSNSSSDSSSSSSYSSSSDSSSSSSGYSSPSYS
                     GSDSSSNTGTGRFLWGLFDPPSPEELERQRQAREQRRIRRKQEAEKRRQQREERRRKE
                     KSRERKKKRQEEAAHTPQWPQGLPKPRPSSSVNSQRQTPPSLQPPTSNVPAGTEAKTL
                     REYLLRIDILRKKSPLSPLEQSLLTELETATRQLWIKASAIIGLTPEERAKLAMSLPI
                     GLDDLATRVGTVTRDALSAWKRNIDANKPPKDDLMVQFNTDKTIALVEYEGSEFAERN
                     LPEEIASKYDTMLGVGKVSIALKKGDRVEAVKESLDMLVGKIGMPQASWAVEGGRMYA
                     NVGFKTLDRFMTDAMAAVGVNFDTKEFWQKFQNDLTFGQKCVYKWIGGPDANE"
     gene            1901..2878
                     /locus_tag="PKO06_05820"
     CDS             1901..2878
                     /locus_tag="PKO06_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69197.1"
                     /translation="MPTNSLKLSCVVLFFALASLVAIAQPAPEIRGALPESLMALPAD
                     GSIKTRWVQPPNPELAETVRQNSPARGGPEFTFSVDASGNPWIGVDGRRLLCPAKGFR
                     ATLSEPWQTFVHLDQGALLLATVRHLGFPALDSQAKSDKDGWPVVAFQPITPLPNYYS
                     RLYKGANDCLYLVSGHATTVKSTLYLLQPETATTGTATGKTVVSRYKKLIETEEPITA
                     VAGDGATTVIAFGRLVVALDAMAGPIFPWPTQPDGEVRELAFLRNVGVFYTTANSVGY
                     LGTKRGFRLLQTPSPMISLAKNSLYVFFPKNYGVMALDNISDLRTSDLP"
     gene            3033..>4600
                     /locus_tag="PKO06_05825"
     CDS             3033..>4600
                     /locus_tag="PKO06_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNV69198.1"
                     /translation="MSHTTAAEVETASPQAHALFRSLWWQYRRPISLGILSLAVTDAM
                     DILPPLIIKRAIDGIEKGESWNLLLWLGVFYLVTAGVQAVFRYFWRTYFIGTSHVIAC
                     DLRGRLFAHLQTLSFGYFNRTKTGELMSRLTNDIEEIRQMFGLGMLLSMDALFYFISV
                     PFIMLWLSPGLALYVLLPMPLIPLFVVKMGRLIHHRSRNVQERLADLSAKTQENISGI
                     RIVKAFCREDEEIASFNSTSRYLVKETLGLARIEAGFHPSLELAMGLGIFLLILLGGY
                     GVVRGEISLGSFVAFQSYLLKMVWPMTAVGMTINLYQRGMASLTRSAEILHTAPEISD
                     QPPPVAAAAAAQASDNPTVVVRPEPEASTIRGDIEVRGLSFTYPESVEPALRDVSFHL
                     PAGKTLGIVGPVGCGKTTLLHLLLRLFDPPAGTIFLDGRDLRSYPVHHLRRELGFVPQ
                     DSFLFSDSILENVLFGMKDRPDPQRARECLRIAQVLAEIEALPQKHESLLGERCSCPP
                     ERLTPRSPSNDSCF"
BASE COUNT          952 a         1444 c         1230 g          974 t
ORIGIN      
        1 cttcttaggg tcatccatgc tctgggcact gccaccgaca atgaaaccgg ttttctcgtc
       61 aatgaaagaa atgtcgaaga gcggaacctt caccccgctg ggaatcgtgg caaccgtgtt
      121 caaattagca tcaaggcgga cgataactcc gtcatacccc acggcccaga tactgccgtc
      181 cacatgccgg gcaatgccac gcaagttcgc cttcactcgc gtgaccttta ccagctcgac
      241 cagcttcgac tgcggaacct gcgaatcccc accttctggt tctgccgtga gtgtccgtgt
      301 cggtctctgg cttgagcatg cgggcaccag attgagcccg agccagacca ccgcaagtac
      361 catcacccat cctcttcgat acgccatcgt gtttgctcct cttcatactg ttcaacgact
      421 ttcatgatac atgagaagtc tttccctggt ctccatgtca tccgcgatcg cttccatcct
      481 cccccttgtg ttcacgaggg tttcgggtgt atcaacgtat tctcgcaatc cgatttgaca
      541 gatctgcgtt tgtcgaccgc ctgtaaggag gaaagaatga aacgacttgc acttgcagta
      601 actctctcaa gtttttttct gttccacggc acacagactc ccacactctg ggcaggtgca
      661 tatgaccaat tgattgacat tggggggccg ccgcccgatg ttcccatgcc gaccaatcca
      721 accccggtcg atccgtcgcc cgataattca aattctcagg acaacagtag tacaagctct
      781 tacaatagca attcgtcatc tgactccagt tcgagcagtt cttattcctc gtcttccgac
      841 tcttcgtctt cgagcagcgg gtattcttct ccgagttact ccggctctga cagttccagc
      901 aacaccggaa ccggcaggtt tctctggggt ctgttcgatc ctccgtcccc agaggaactc
      961 gagcgacaac gccaggcccg cgaacagcgg cgcattcgcc gcaagcagga agcggagaaa
     1021 cgacgacaac agcgtgaaga acgccgtcgc aaagagaaga gccgcgagcg aaaaaagaaa
     1081 cgccaggagg aagctgccca cacacctcag tggccgcaag ggcttcccaa acctcgtcca
     1141 tcctcttctg tgaattccca gcgccagaca ccgcccagtc tgcagccacc gaccagcaat
     1201 gtcccagccg gtaccgaagc aaaaacgctt cgagagtatc ttctgcgcat cgacattcta
     1261 cgcaagaagt cgccgctgtc accgctcgaa cagagcctgc tgacagagct cgaaaccgcg
     1321 acgcgacaac tgtggatcaa ggcgagcgcg atcattggcc tgacgccgga agaacgcgcc
     1381 aaactggcca tgagtctgcc tatcggtctc gacgatctcg ccaccagagt gggcacggtc
     1441 acgcgcgacg ccctgagcgc ctggaagcgg aatatcgacg cgaacaaacc gccgaaagac
     1501 gatctcatgg ttcagttcaa caccgacaaa accattgcac tcgtcgagta tgagggcagc
     1561 gaattcgccg agcgcaacct gcctgaggag attgccagca agtacgatac gatgctgggc
     1621 gttggcaagg tgagcatcgc gctcaagaag ggtgatcgag ttgaggcggt caaagagtct
     1681 ctcgacatgc tggtcggcaa gatcggcatg ccgcaggcat cgtgggcagt cgaaggcggg
     1741 cgtatgtatg ccaacgtcgg attcaagacg ctcgatcgct tcatgaccga cgccatggcg
     1801 gcggtaggag tcaatttcga taccaaggaa ttctggcaga agttccagaa tgacctgact
     1861 tttggccaga aatgtgtcta caagtggatt ggaggacccg atgccaacga atagtctcaa
     1921 actctcctgc gttgtccttt tcttcgccct ggcaagcctg gtcgcgatcg cccaaccggc
     1981 accggagatc cgcggagcgc ttccggaatc gttgatggca cttccggctg acgggtcgat
     2041 caaaactcgc tgggtacaac cgccgaatcc cgaactcgcc gaaacggtgc ggcagaactc
     2101 cccggcgcgt ggcgggcccg aattcacctt ctccgtcgat gcctccggta acccctggat
     2161 tggcgtggat ggcaggcgct tgctgtgccc ggcgaaaggg tttcgtgcga cgctttctga
     2221 gccctggcaa accttcgtac atctcgacca gggagccctg ctgctggcaa cggtgcgtca
     2281 tctcgggttt ccggcgctcg attcccaagc gaagagcgac aaggacggct ggcccgtcgt
     2341 cgcctttcag ccgatcacgc cgctgcccaa ttattacagc cgcttgtaca agggggccaa
     2401 tgattgtctc tatctggtca gcggccacgc cactaccgta aaatcgacgt tgtatctgtt
     2461 gcaacctgag acggcaacca ccggcaccgc cacgggcaag acggtggtct ctcgctacaa
     2521 gaaactgatc gagaccgaag agccgatcac cgcggtcgct ggcgatggcg cgacgacggt
     2581 cattgccttc ggtcgcctcg tcgtcgctct cgatgcgatg gccggaccga tcttcccctg
     2641 gccgacccaa ccagacggtg aggttcgcga actggcgttc ctgcgaaatg tcggggtgtt
     2701 ctacacaacc gccaacagtg taggatatct cggaaccaaa cgcgggtttc gcctgctgca
     2761 gactccttcg ccgatgatca gtctggccaa gaacagcctg tacgttttct tcccgaaaaa
     2821 ctacggtgtg atggccctcg acaacatcag cgacctccgc acatccgact tgccctgacc
     2881 gtccgacaga agcgagtttc aggcccggcg agcgtcagct cgccgggcct gttgtctccg
     2941 agcctgattc aacctccctc ttcccgggcg agcggtttga cagaatgggc ctgtagagag
     3001 tattctgagc cacttttccg gcagaagaat ccatgtcaca caccaccgca gccgaagtcg
     3061 aaaccgcctc cccacaggca cacgcgctgt tccgcagtct gtggtggcag tatcggcgcc
     3121 ccatcagtct gggcatcctg tcactggccg tcaccgatgc catggatatt cttcccccat
     3181 tgatcatcaa acgtgcgatc gacggcatcg aaaaaggtga aagctggaac ctgctgctgt
     3241 ggctgggtgt cttctacctc gtcacggcgg gagtgcaggc cgtgttccgc tacttctggc
     3301 gcacctattt catcggtaca agtcacgtca tcgcctgcga ccttcgtgga aggctgtttg
     3361 cccatctgca aactctgtcg ttcgggtatt tcaatcgaac gaagaccggc gaactcatgt
     3421 cgcgactcac caacgatatc gaagagatcc gacagatgtt cgggctcggc atgttgctct
     3481 cgatggacgc gctgttctac ttcatctcgg ttccgttcat catgctctgg ctttcgcccg
     3541 ggctggctct ctatgtcctg ctgcccatgc cgctcatccc gctcttcgtc gtgaagatgg
     3601 gaagattgat tcatcatcgc tccaggaacg ttcaggagcg cctcgccgat ctgtcagcca
     3661 aaacccagga aaacatctct ggcattcgca tcgtcaaggc gttctgccga gaggacgaag
     3721 aaatcgcgtc gttcaacagt acaagtcgat atttggtcaa agagacacta ggtcttgctc
     3781 gtatcgaagc aggttttcac ccgtcgctcg aactcgccat gggcctgggc atctttctgt
     3841 tgattctgct gggcgggtat ggcgtggtgc gaggtgagat ctcacttggg tcattcgtcg
     3901 cgtttcagtc ctacctgctc aaaatggtct ggccaatgac cgccgtcggc atgactatca
     3961 atctttacca gcgcggtatg gcttcattga cccggtccgc cgagatcctg cataccgccc
     4021 cggaaatcag cgatcaaccg ccacctgtcg cagccgctgc cgcggcccag gcatccgaca
     4081 acccgaccgt cgtggtccgc cccgagcctg aggcgtcgac cattcgtgga gacatcgagg
     4141 tgcgtggcct gtccttcacg tatcccgagt ccgtcgaacc ggcactgcga gatgtctcct
     4201 tccatctgcc ggccggaaaa acgctcggca tcgtcggccc cgtcggttgc ggcaagacca
     4261 cgcttcttca cctgctgctg cggctgttcg atccacccgc cggcacgatt tttctcgacg
     4321 gacgcgatct tcgctcttac cccgtccatc atctgaggcg cgagctcggc ttcgtccccc
     4381 aggactcgtt cctgttttcc gactcgattc tcgaaaatgt cctgttcggc atgaaggaca
     4441 ggcccgatcc gcagcgggca cgcgagtgtc tgcgcatcgc gcaggtgctg gccgaaatcg
     4501 aggcactgcc gcagaagcac gagtcgttgc tcggcgaacg ctgctcctgc ccgcccgaga
     4561 gattgacgcc gcgttcgccg agcaacgact cgtgcttctg
//
LOCUS       DAORHD010000212         4594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19895_length_4594_cov_3.106411, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000212 DAORHD010000000
VERSION     DAORHD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19895_length_4594_cov_3.106411"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1721
                     /gene="topA"
                     /locus_tag="PKO06_05830"
                     /pseudo
     CDS             15..1721
                     /gene="topA"
                     /locus_tag="PKO06_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
     gene            1777..2046
                     /locus_tag="PKO06_05835"
     CDS             1777..2046
                     /locus_tag="PKO06_05835"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNV69199.1"
                     /translation="MPAWPVRWPVGGKVVVKRSKRARTFYGCSNYPKCMLTTWSKPTG
                     ALCPTCQAPLVHHATKKLGEHVKCSNKACQYKLIPEGEAESGKKA"
     gene            2030..3370
                     /gene="trmFO"
                     /locus_tag="PKO06_05840"
     CDS             2030..3370
                     /gene="trmFO"
                     /locus_tag="PKO06_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018750905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNV69200.1"
                     /translation="MVKKPSPPPVTVIGAGLAGSEATFQLIRRGIRVRLFEMRPKVPT
                     GAHRTDLFSELLCSNSMGSNLPDRASGLLKEEMRALNSYIMDTAYKYRVPAGQALAVD
                     RDAFAREITRYLRGHSLVEFINDEVTEMPTEGMVIIASGPLTSKAFLDHLAKTFGTEK
                     LFFFDALAPVVTAASIDMTKAFKADRYGTGEDEGSYINCPLSKEEYELFVKELLAAEK
                     LPLPLHEKEAKKQFFQACQPIEEIAATGVDSLRFGPFKPVGLTDPITKKKAYAVLQLR
                     QDNLLASLYNLVGCQTNMKYGEQERVLRLIPALKNAEFVRLGQMHRNAYLYSPKMLTG
                     TLQIQGKPEWFLAGQITGVEGYSESTAMGLLAGLNAARIALGKQPVVPPPESIMGALV
                     KYITFPGHKQFSPVNANFGLFTDLVADDTVRGDARKGLVLTNARRAFRQFVTEL"
     gene            complement(3514..>4594)
                     /locus_tag="PKO06_05845"
     CDS             complement(3514..>4594)
                     /locus_tag="PKO06_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69201.1"
                     /translation="TSHAQYATGPTNIPIYRLQPLEFIAINAITTDHEMGGAVSTISW
                     TILGEDDFVENATGTTYSSNLDPGSYTIILRPQDNFGFWGEASKVIWIMEFEQTTPGI
                     ASPTGITAQPDGSAVFVTEEINPTTWQMSRFSREPGIDEGTGGDLTLTHSEGSASLSS
                     QLRDIKAMDNANVFSLEADGTISRFATADLASNATPVTCSVVNPTTFAYGADSFLYVT
                     DVTAGRLYKVNPSTGAITASPTMGNIFGVAYFDSTTDMLYVTQPASSLVRKYNLSYTI
                     QPFTGTGVTGPLYTTTSAKYVIVSNGSNSLSFFDHSTGTLQFTMDGFTTARGVVVVGP
                     DLYVVENTPNRITRYRFGRYDASQW"
BASE COUNT         1053 a         1331 c         1248 g          962 t
ORIGIN      
        1 tcagcccgtg ccgcgtgacc ttcaacgcga tcaccgagaa cgaggtccga aacgctatcg
       61 ataatccccg accgattgat ctgaatctag tcagcgcgca gcagtcacgc cgcattctcg
      121 atcgcctggt cggctataaa ctgtcccccc tgctttggaa gaaggtccag atggggcttt
      181 ccgccggtcg cgtccagtcc gtcgccgtct ggttcatctg cgaacgcgag aaagaaattc
      241 tggcgttcaa ccccgaagaa tactggacct tcaaagcgtg ggtacaagat gatgccggcc
      301 atggcttcga aaccaagctg gtcaagatcg atggcaaaaa gcccgcggtt ggaaatcagc
      361 aagacgccga agcgattcgc gccgagattc aagccgccgc atcgctgcag gtcggaagtg
      421 tcagtcggcg agagcgccgt cagacgccgc cgccgccttt tatcaccagt actctgcagc
      481 aggaagcatc gcaacgtttt tccttcacgc cgaagcgaac gatgtccatt gcccagaagc
      541 tgtatgaagg tctcgaagtt ggcgaagacg ggccagtcgg tctgatcacg tatatgcgaa
      601 ccgattcggt gcgtatcgct ccggaggggc tggccgaagc caaggatttc atcgctcatc
      661 gttacggccc cacctacgtc ttgagtcaac ctcgcttgta caaaaccaaa aaaggcgctc
      721 aggatgccca cgaagcaatt cgacccaccg cggtcaagcg aaccccggaa tcactgacgc
      781 gcttcctttc cgccgaggaa ctgaaactgt ataccctgat ctggcgacga tttgttgcct
      841 ctcagatggc ggaagccgtc tttgacgtcg tgaccatcga cattcccgta tcgcgctatc
      901 agatgcaggc ggtcggcacg acgctgaaat ttgatggttt ctcaaccctg tacaccgaga
      961 cgcgcgtcga gggccaatca gaagacaacg gcgaggaaag cgcggaagac ggcaaacagc
     1021 gactcccgga agtcgtcgaa ggacagacat tgttgttgca gcggattgaa ccgaagcaga
     1081 atttcaccgc tccgccaccg cgctactctg aagccatgct gatcaagacg ctcgaaaagg
     1141 aaggcatcgg acgaccgagc acctactcga cgatcgtcga cacgattcaa cagcgtgggt
     1201 acgtgaaaaa gatcgagggt cgtttccgac cttctgactc ggcgtttctc tcgaccgaga
     1261 ttctcacctc ctgtttcggt gatgtcatca acccgcgttt caccgccgac atggaatcac
     1321 tcctcgacga ggtcgaagag gctcagaaag actgggtgca gattcttcgt gatttctatg
     1381 tgccctttgc tcgcgacctc aagaaggccg atggcgagct gaagaaggtc gaaatggtct
     1441 cggactgtat ctgccccaag tgtggtcgcc agatggtggt caagttcgga cgcgccggca
     1501 agtttctggc ctgccccggg tatcccgagt gcaaggaaac cgaaaacatc ccggaagaac
     1561 tgctgttgtt ctcgtcaaac cccaccggcg aaacggtgca cctgcgcgcg aaactcgacc
     1621 gtatcaaggc cgagaatccg ccgccaccca tcgaaccctc tgaccaagtc tgcgaattgt
     1681 gtggtgctca gatgatcgtg cgctcgggga agttcggccg gttcctggcg tgcagtgcgt
     1741 atccgacctg caaagccacc aagcccatcc tgcaggatgc cggcgtggcc tgtccgctgg
     1801 ccggttggtg gcaaagtcgt ggtcaagcgc tcgaagcggg cacgcacgtt ctacgggtgt
     1861 tccaactacc cgaaatgcat gctgaccacg tggtcaaaac cgactggcgc tctctgtccg
     1921 acctgtcagg caccgctggt tcaccatgcc accaagaaac tgggcgaaca cgtgaagtgt
     1981 tcgaataaag cctgccagta caagctcatt ccagaaggag aagccgagag tggtaaaaaa
     2041 gcctagcccc ccacccgtca cggtcattgg tgccggtctg gccggcagcg aagccacttt
     2101 tcaactgatc cggcgcggca ttcgcgttcg tctgttcgaa atgcgaccga aagtgcccac
     2161 gggcgcgcac cgcaccgatc tcttcagcga gctgctgtgc agcaattcca tgggcagcaa
     2221 cctccccgat cgcgcatctg gtctactcaa ggaagagatg cgcgccctca acagctacat
     2281 catggacacg gcctacaagt accgagtacc agccggccag gcgcttgcgg tggatcgcga
     2341 cgctttcgcg cgcgagatca cccgctacct gcgtggtcac agtctggtcg agttcatcaa
     2401 tgatgaagtg accgagatgc cgaccgaggg catggtcatc atcgcttctg ggccactgac
     2461 ctcgaaggcg tttctcgatc atctggcgaa aacgttcggc actgaaaagc tcttcttctt
     2521 tgacgcgctc gctcccgtcg tgacggcggc gtcgattgac atgacgaagg ccttcaaggc
     2581 tgatcgttac ggcaccggcg aagacgaggg ctcgtacatc aattgcccgt tatcgaaaga
     2641 agagtacgag ctgttcgtca aggaactgct ggcggcagag aaacttccgc tgccgctgca
     2701 cgaaaaagag gctaagaaac agtttttcca ggcgtgccag ccgatcgaag agatcgctgc
     2761 caccggcgtc gattcactgc gtttcggtcc cttcaagccg gtcggactga ctgatccgat
     2821 cacaaagaag aaagcctacg ccgttctgca gttgaggcag gacaacctgc ttgccagctt
     2881 gtacaatctg gtgggttgcc agacgaacat gaagtatggc gaacaggaac gcgtcctgcg
     2941 tttgattccg gcattgaaga atgccgagtt tgtccgactc ggacaaatgc accgcaatgc
     3001 ctacctgtat tcgccaaaga tgctgaccgg cacgctgcag attcaaggca agcccgagtg
     3061 gtttctcgcc ggtcagatta ccggcgtcga gggatacagc gaatcgaccg cgatgggcct
     3121 gctggccggc ttgaacgccg cccgcatcgc cttgggcaag caaccggtcg tgccgccccc
     3181 cgagtcgatc atgggcgctc tggtgaagta catcaccttc ccgggccaca agcagttctc
     3241 gccggtcaac gccaacttcg gtctgttcac cgatctggtg gccgacgaca cggtccgcgg
     3301 cgacgcccgc aaaggcttgg ttctgaccaa cgcccgccgc gctttccgcc aattcgtcac
     3361 cgaactgtaa ttccccccgt cagcaatagc cccgagtgcc ccacgacaac tcggggctgt
     3421 tcgtttcagc agtgtgaaac ctcatttcgc tttcaaacac accggccccg gaagtgattc
     3481 cggggccggt gtttcaaaac tgcctgtttc tctttaccat tgcgatgcgt cgtagcgtcc
     3541 gaaacgatat cgagtgatcc gatttggcgt attctcgacc acatacaagt cgggccccac
     3601 aacaacaaca cctcgcgcag tcgtaaaccc atccattgtg aattgtagtg tcccagtcga
     3661 gtgatcaaag aacgaaaggg aattcgatcc gttcgaaacg atcacgtatt ttgcagacgt
     3721 cgtcgtatac aatggcccag ttacgccagt tcctgtgaat ggctgtatcg tataactcaa
     3781 attgtatttg cgaaccaggc tagaggccgg ttgggttaca tacagcatat cagtggtgga
     3841 atcgaagtag gccacgccaa aaatgttccc catcgtcgga cttgccgtaa tggctcccgt
     3901 agacgggttc accttgtaca aacgaccagc tgtcacatcc gtcacataca ggaaactgtc
     3961 ggcaccatac gcaaaggtcg ttgggtttac cacagagcac gttactggcg ttgcgtttga
     4021 agcaagatca gcagtcgcaa atcggcttat ggttccgtcg gcttccagcg aaaagacatt
     4081 tgcattgtcc atggctttga tgtcccgcag ctggctcgag agagacgcac taccctcaga
     4141 atgggtaagc gtgaggtcac ctccagttcc ttcatcgatg cctggttctc ttgagaaacg
     4201 cgacatctgc caggtcgttg ggttgatttc ctcagttacg aacactgcac tgccatcagg
     4261 ttgtgcggta atacctgttg gacttgcaat tccaggcgtt gtctgttcga actccatgat
     4321 ccagatcact ttcgaagcct cgccccagaa accaaaatta tcttgcggtc ttaagataat
     4381 ggtgtatgaa cccggatcga gattgctcga ataggttgtt cctgtagcat tttcgacaaa
     4441 gtcatcctcg ccaagaatgg tccaggaaat ggtagacaca gcacctccca tttcgtggtc
     4501 agtcgtaatg gcattaatgg caataaactc aagcggttgc agacgataaa taggaatgtt
     4561 ggtggggccg gtggcatact gggcatggct ggtc
//
LOCUS       DAORHD010000213         4582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_19970_length_4582_cov_3.765408, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000213 DAORHD010000000
VERSION     DAORHD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19970_length_4582_cov_3.765408"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /gene="glgP"
                     /locus_tag="PKO06_05850"
     CDS             complement(<1..1334)
                     /gene="glgP"
                     /locus_tag="PKO06_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNV69202.1"
                     /translation="MKSIKNFSVKPRLPQALAKIHDLAYNLWWTWNPEAIDLFRRLDP
                     TEWEHTYHNPVKMLGTIAQEVLEERAQDESFLAHLHRVANDFQAYMSSKQTWYGKQYG
                     VPETPQIAYFSAEFGIAECLPLYSGGLGMLAGDHLKSASDLGLPLAGVGLLYRVGYFQ
                     QYLNAGGWQQELYPENDFYNMPITPLLDEKGNQLFVKVQLPGREVFVLLWKAQIGRIP
                     LILLDCDTTRNQPQDRRITGELYGGTIETRIQQEIVLGIGGLRALHALNYHPPVYHMN
                     EGHSAFLALERIRVAMERHSMSFSDAHELTRSSNLFTTHTPVPAGIDVFSGELMDRYF
                     SSYAPKLGISWQDFLNLGWQTNTPKGDGFSMAVCAINLAAHCNGVSVLHAKVSREMWK
                     DMFPGFMVNEVPICSVTNGVHQRSYISKEMSQLYDRYLGPKWLQPPAGNPVW"
     gene            1467..2165
                     /locus_tag="PKO06_05855"
     CDS             1467..2165
                     /locus_tag="PKO06_05855"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNV69203.1"
                     /translation="MNFRTLFSVPLVLLAAVMTAGALERTASDPFLIPLQEPEMVISA
                     ESLDLKAGIVTLRGKVRAVRGTDVLTCEKALLRNKPQWLLASLTPKLVRKESLAEKMV
                     LRESQLEAENIVWEEATNRVLASPAVTIRIEERSWDLATYTWAVISADAMEGIRDQQI
                     MNFEGNVRMRDKTRYGEGQKLEYFKASSTVILSGQARIESEEFDAKNNKTVKRQINGN
                     RIIYNMKTKEAFSE"
     gene            2170..4473
                     /locus_tag="PKO06_05860"
     CDS             2170..4473
                     /locus_tag="PKO06_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV69204.1"
                     /translation="MAVNITFRNYVLDPFQIQAITEIEQGNSLVVSAPTGSGKTLVAE
                     YLIEKMLQTSRRIVYTSPIKALSNQKYRDFSRLYGDKIGILTGDVVVNRDAQVLIMTT
                     EVYRNMVVEDPEAVKDVAYVIFDEIHFLGDIERGTVWEESIIFSPKTVRILALSATIP
                     NCEELARWIESVLDHRVGVISHNQRVVPLSFLYYHQAALVPYKELVRRVSKRGQSESE
                     DGGRRSRKGKEEESRRHLDVIRTLRSQDRLPALYFVFSRALADHLSQETARIIDFTSA
                     EEKAYIDQQADECFAKYGIADLESAQTMKEMLRRGVGKHHAGILPQLKELVEVLFAER
                     LLRVLFVTETFAVGVNMPARTVVFDALKKFDGRSFRVMKTLEFYQISGRAGRRGIDPQ
                     GWVVVPYLPKDLDLKDMETLVYGDVEPLLSQFDLSFNSVLNLYAGHKSDEVRLILKRN
                     FAQFQANKELPKLTDMVARLRAEIDELYPRCMQKKNDLDVYIAFHRKKQDMMNSWHRQ
                     LDATFQGLRGRKSRGVRQAMQEKFQAKMAALEEEERQYLCGACPSRGKCTSRIYKIFK
                     VDKKLDYWRGLLEQQEELQLPLYEEKLGILREFGYIDDRGLLARGEFASRIHVEELAV
                     TELYFRGVFHEWDHHEINALAHALTFERRRGVEAAPPPKGQALNRLREARGYVNSLAR
                     RIKYVKQLDITLAMVMHAWSKGADLEQIASMTNQSEGDMIRSFRQAIDLLRQIRDATD
                     DKSLKEKLTDCVYLINRDIVLATELRD"
BASE COUNT         1024 a         1225 c         1360 g          973 t
ORIGIN      
        1 ttccacacgg ggttcccggc tggcggctgc agccatttgg ggccgagata gcgatcatac
       61 aactgggaca tttccttgga gatgtaagac cgctgatgga cgccgttcgt gaccgaacag
      121 atcgggactt cgttgaccat gaagccgggg aacatgtctt tccacatttc ccgtgaaacc
      181 ttggcatgca gaacgctgac gccgttgcag tgcgcggcca gattaatcgc gcataccgcc
      241 atcgagaaac cgtcaccttt gggggtgttg gtctgccagc cgaggttcag gaagtcttgc
      301 cagctgatac caagtttcgg cgcgtacgaa gaaaaatagc gatccatcag ttcacctgag
      361 aacacatcga ttccagcggg aaccggggtg tgcgtggtga acaggttgct tgaacgagtc
      421 agttcgtgcg cgtcgctgaa gctcatgctg tgtcgttcca tggcgacccg aatgcgctcg
      481 agcgcgagga acgcggagtg cccctcgttc atgtggtaga ccggtggatg atagttcagg
      541 gcgtgcaggg cacgcagacc gccaattccc aacacgattt cctgctgaat gcgggtttcg
      601 atggtgccac cgtagagttc gcccgtgatt cggcgatctt gcggttgatt gcgtgtggtg
      661 tcgcagtcga gcagaatcag cggaatgcgc ccgatctggg ctttccagag cagaacgaag
      721 acttcgcggc ccggcagctg cactttgacg aacagctgat tccccttttc atcgagcagt
      781 ggggtgatcg gcatgttgta aaaatcgttc tcggggtaga gttcctgctg ccagccgccc
      841 gcgttcagat actgctgaaa gtatccgacg cgatagagca acccgacgcc tgcgagaggc
      901 agaccgagat ctgaagcgga tttcagatga tcgccggcca gcatgcccag gccgcccgag
      961 tagagcggca gacattcggc aatcccgaat tccgcggaaa aataggcgat ctgaggtgtc
     1021 tcgggcacgc catactgctt tccataccag gtttgcttcg agctcatgta cgcctgaaaa
     1081 tcgttggcga cacggtgcag atgggcgagg aagctctcgt cctgagcccg ttcttcgagg
     1141 acttcctggg cgatcgttcc gagcatcttc accggattgt ggtaggtgtg ttcccattcc
     1201 gtcgggtcga gacgacgaaa caggtcgatc gcctccggat tccaggtcca ccaaaggttg
     1261 taagccaggt cgtgaatctt ggccagggcc tggggaagtc gcggtttgac cgagaagttc
     1321 ttaatggatt tcatacgtcc tccgaaaatt ggggggtgta aacggagtct ttataacacg
     1381 aagctgtcaa ccctgccaaa ccctcagaat ctggtctgta ttggaaaacc ctcgcggacc
     1441 tgctataatc tgctcgtcac gactccatga atttcagaac tctcttttcc gttccgcttg
     1501 tgcttctggc ggcggtgatg acggcgggcg ccctcgagcg aacggcctca gatccctttc
     1561 ttattccgct gcaggagccg gagatggtca tttccgccga gtcgctcgat ctgaaggcgg
     1621 gcattgtgac cctgcgcggc aaggtgcggg cggtgcgcgg cacggatgtc ctgacctgcg
     1681 agaaagctct gctgcgaaac aagccgcagt ggctgctggc ttcattgaca ccgaagctgg
     1741 tacgcaaaga atcgttggcc gaaaagatgg ttctgcgcga gtctcagctc gaagccgaaa
     1801 acatcgtctg ggaagaagcg accaaccggg ttctggcttc gccggccgtg accattcgca
     1861 tcgaggagcg cagctgggat ctcgccactt acacctgggc cgtgatttcc gccgatgcga
     1921 tggaaggaat tcgtgatcag cagatcatga acttcgaggg caatgtgcgc atgcgcgaca
     1981 agacgcggta tggtgagggg cagaagctcg aatacttcaa ggccagctcg acggtgattc
     2041 tgtctgggca ggcacgaatc gagtcagaag aattcgacgc gaagaacaac aagacggtga
     2101 aacggcagat caacggaaac cgaatcatct acaacatgaa aaccaaggaa gcgttctcag
     2161 agtaacacta tggccgtaaa catcaccttt cggaactacg ttctcgaccc gtttcagatt
     2221 caggcgatca cggagatcga acaggggaac tccctcgtcg tctcagcgcc gaccggctcc
     2281 ggcaagacac tggtggccga gtatctgatt gagaagatgc tgcagaccag ccgccgcatt
     2341 gtgtatacct ccccgatcaa agcgctctcg aaccagaagt atcgcgattt ttcgcgactc
     2401 tatggcgaca aaatcggcat tctgacgggt gatgtcgtgg tgaatcgcga tgcgcaggtg
     2461 ctgatcatga ccacggaggt ctaccgcaac atggtggttg aagatcccga ggccgtcaaa
     2521 gacgtggcct acgtgatctt tgacgagatt cactttctcg gcgatattga gcgcggcacg
     2581 gtctgggagg aatcaatcat cttctcgccg aagaccgtac gcattctggc gctgtcggcg
     2641 acgattccga actgtgaaga actggcgcgc tggatcgagt ccgttctcga tcatcgtgtt
     2701 ggcgtaatct ctcataatca gagggttgtg ccactttctt tcttgtacta tcaccaggca
     2761 gcgctggtgc catacaaaga actggttcgc cgggtgtcaa agcgcgggca gtcagagtcc
     2821 gaggatggag gacgcagaag tcgcaaagga aaagaggaag agagtcggcg acatctcgat
     2881 gtgattcgaa cgctgcgttc gcaagaccgt ttgccagcgc tgtatttcgt cttttcgcgc
     2941 gcgctggccg atcacctgtc tcaggaaacc gcccggatca tcgattttac cagcgccgaa
     3001 gaaaaagcct atatcgatca gcaggctgac gaatgctttg ccaaatacgg cattgctgat
     3061 ctcgagagcg ctcagaccat gaaagagatg ctgcgtcgcg gggttggcaa gcatcatgcc
     3121 ggtattctgc cgcaactgaa agaactggtc gaagtcctgt ttgcggagcg tctgctgcgc
     3181 gtgctgttcg ttaccgagac gttcgccgtc ggcgtgaata tgccggcgcg cacggtcgtg
     3241 ttcgatgccc tcaagaaatt cgacggtcgc tcgttccggg tgatgaagac gcttgagttc
     3301 tatcagatct cgggtcgcgc cggacgacgt ggcatcgacc cgcaggggtg ggtcgtggtt
     3361 ccgtatctgc ccaaagacct cgatctcaag gacatggaaa cgctggtcta tggagatgtc
     3421 gagccgttgc tgagccagtt cgatctgtcg ttcaacagtg tgctcaacct gtatgccggt
     3481 cacaaatccg acgaggtgcg actgattctc aaacgcaact ttgcccaatt ccaggcaaac
     3541 aaggaattgc cgaagctcac ggatatggtt gccaggcttc gggccgagat cgacgagttg
     3601 tacccacgtt gcatgcagaa aaagaatgat ctcgacgtgt acattgcctt tcaccgcaag
     3661 aaacaggata tgatgaactc ctggcatcgt cagctcgatg ccacctttca gggattgcgt
     3721 ggtcgcaagt cacgcggagt gcggcaagcc atgcaggaaa agtttcaggc gaagatggcc
     3781 gcactcgagg aagaggaacg gcaatatctg tgcggcgcgt gtccgagtcg cggcaagtgt
     3841 accagtcgca tttacaagat cttcaaggtc gacaagaaac tcgactactg gcgaggcctg
     3901 ctcgaacaac aggaagaact gcagttgccg ttgtatgaag agaagctcgg tattctgcgc
     3961 gagttcgggt atatcgacga tcgcgggttg ctggcgcgtg gagagttcgc ctcgcgcatt
     4021 cacgttgagg aactggcggt taccgagttg tactttcgcg gggtctttca tgaatgggat
     4081 caccacgaga tcaacgccct ggcccatgcg ctgaccttcg agcgtcgacg cggtgtcgag
     4141 gcggcaccac cgcccaaggg gcaagcgctc aatcgcctgc gcgaggcgcg cgggtatgtg
     4201 aactcattgg cccgtcgcat caagtatgtg aagcagctcg atatcactct ggcgatggtg
     4261 atgcatgcct ggagcaaagg agccgatctc gaacagatag cgtcgatgac caaccagtcc
     4321 gagggcgaca tgattcgctc tttccgacag gcgatcgatc tgctgcgcca gattcgggat
     4381 gcgacggacg acaagagcct gaaggagaaa ctcaccgatt gcgtctacct catcaaccgc
     4441 gatatcgttc tggcaaccga actgcgcgat tgagctacga cctcggaata ccgcgatgac
     4501 gaccccgcca ccacccgaag aatttttcag ttattccgcg gacgacacgg tcgaccatgc
     4561 ccgggcgctg ggacagttgt at
//
LOCUS       DAORHD010000214         4567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20058_length_4567_cov_3.777039, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000214 DAORHD010000000
VERSION     DAORHD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20058_length_4567_cov_3.777039"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKO06_05865"
     CDS             <1..297
                     /locus_tag="PKO06_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNV69205.1"
                     /translation="IPALQKAPMNSVHEWGSGMSFIRDFENGKSYAVVGLAAGGDQQI
                     TVVGVRNGTYRDAVTGNVAQVGNGTLSFHVKGNSAGIYVLNGPGKIGQDGPFLK"
     gene            complement(368..1279)
                     /locus_tag="PKO06_05870"
     CDS             complement(368..1279)
                     /locus_tag="PKO06_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV69206.1"
                     /translation="MSWIGLGFASAVLLGGYEIAKKCALRSFSAMQALFWSSVVNAVL
                     MVPEWATHAPLLTRPAHAWVLLKALVVSCSWLCAFAAMKHLPISVVTPMRAFSPMFTA
                     LGGVFLLGEHLLVRQWAGLLVCAGGFSRLARQSADEGVAWQTNRWIVVYTVSLAFGVG
                     AALLDKTIMSRPDLPGSAFDRARAMQIWFSFYTPLFLFPLFRLSQLYPNDTAANTRIQ
                     DPPSVPSTAWIFLPLISLALLAADRCYFTALGEPEAHISIVSCIRQSCILVSAIGGGW
                     LLGEMNLLRKASSVLLIVGGLALLLVH"
     gene            1405..3348
                     /locus_tag="PKO06_05875"
     CDS             1405..3348
                     /locus_tag="PKO06_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV69207.1"
                     /translation="MKYRVLVADADLSSLNETQRELTSRGLSVAVAATFNEACRLLRE
                     DGLIHVMLTELSLPEDETPDAPIMTGPEMFEEFLRIRYEVNIFLLTAEKAGRRVVTGG
                     LLNGYFYKGDHDFDDVIRKVRTEVISSKNRAPFFETLVEYAEKAKDSWHTPGHASGDS
                     IRNSVWVKEYHDFFGPNVFRSDVSVSVPMLDSLLNPTGVIKEAQELAARAFSSRYTFF
                     STNGTSTSNKILLQTLLKPGDAILLDRNCHKSVHYGVIISGAEPIYLLPSANQTYGIF
                     GPVPKQRILTTMDQALAAGKRVKALILTNCTYDGLTYDLPPIIAEAHKRGIKVIIDEA
                     WYGYARFHPAFYPCAMEAGADYATQSTHKTMSAFSQASMIHVNDPDFEQIQDFFYENF
                     NMHTSTSPGYPMIASLDAARKQMVMEGYSLLSRCLDLAESFRQAINSLRKFRVLSLED
                     MLTDEIRHDHVRLDPTKITIDCSRSGFTSKEVERILLSKHNIQIEKSTFNTLTVLLTI
                     GATQSKLTRLFLALENIERMGGNVSDDPHLMPREFRLSLSAMKFMPRHAFYCDGEKVN
                     LREAEGKVSTVMVVPYPPGIPLLVPGQTVTGEIIKALEIYRDHHVEIHGLHEGMIKVM
                     TPPEENRLAERGHAISPLEETRA"
     gene            3345..>4567
                     /locus_tag="PKO06_05880"
     CDS             3345..>4567
                     /locus_tag="PKO06_05880"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNV69208.1"
                     /translation="MTHFGLISLLPAAVTILVAVVSQRIVLAIFLGIVAGSLGATAGA
                     WDPAWVKVEQYLSVSFQDLERLKIAAFVIMMGALVEVIARSGAYLKLSEAFRRFLNTP
                     RKGRLASIGLGGIVFFDDYANLLISGTSMRGLGTALRISPGLHGYLVDQLATVVSLMF
                     ISTWAAYEGSLMTGAAEMVGLKSTPTQMLVDSLPYHVFTILGLFLALVVAWEGRWFGA
                     AFDAEPDKAVRPVNVVGVGARLWHGLLPLMTLVGVTVWGVYTLAMEAQIKLGPAATWV
                     NLLAEVPTINVLLFATAAGHMVALLTLVPSAVLMPFQYRKAIFEGCCHMIPAGLVIIL
                     AKGLSLVAGDLGTGTYITELLKGLAVPAVVPALVFVVSFLISVATGFSWSSMAIVMPV
                     AYQMAMGVGGASMIP"
BASE COUNT          925 a         1278 c         1407 g          957 t
ORIGIN      
        1 attcccgctc tgcagaaggc accgatgaac agcgtgcacg agtggggttc gggcatgagc
       61 ttcattcgcg actttgagaa cggcaagagc tatgccgtcg tcggtctcgc ggcgggcggc
      121 gatcagcaga tcaccgtcgt tggcgtgcgc aacggcacgt atcgtgatgc cgtcaccgga
      181 aacgtcgcgc aggtcggcaa tggcaccctg agctttcatg ttaagggcaa ctcggccggt
      241 atttacgtgc tgaacggccc tggcaagatc ggacaggatg gtccgtttct gaaatagtcg
      301 aagccgaaga gcagccgggg cgggcgtcgt tgcgacgaac ccgccccggg cacttttcgg
      361 aagttcttca gtgaacgagc agcagtgcga gaccgcccac gatcaggagc acggaactgg
      421 ctttccggag caggttcatt tcgcccagca gccacccgcc cccgatggcc gagacgagaa
      481 tgcatgactg gcgaatgcag gaaacgatgc tgatgtgtgc ctccggttcg ccgagtgccg
      541 tgaagtaaca gcggtcagcc gccagcagag ccagggatat cagcgggaga aagatccacg
      601 cggtcgatgg aactgaggga gggtcctgaa tccttgtgtt ggcggcagta tcgttcgggt
      661 ataactggga caagcgaaac aagggaaaga ggaacagagg ggtgtaaaaa ctgaaccaga
      721 tctgcatggc ccgggctcga tcaaaggcac tccccggcag atccggccgg ctcatgatcg
      781 tcttatcgag caacgcggct ccaaccccga atgccagact gaccgtgtag acgacgatcc
      841 agcggttggt ttgccaggcc acgccctcgt ccgcggactg gcgggcgagt cgggagaagc
      901 ctcccgcgca gaccagcaga cccgcccact gcctgacgag caggtgctct ccgagcagga
      961 atacgccgcc gagtgccgtg aacatggggc tgaacgcccg catcggcgtc acgaccgaga
     1021 tcggcagatg cttcatggcg gcaaaggcac acagccagga acagctgacc accagagcct
     1081 tcagcaatac ccaggcatgc gcggggcgcg tcagaagcgg tgcgtgtgtc gcccattccg
     1141 gtaccatcag cacagcgttg accacggaac tccagaacag ggcctgcatg gcggaaaacg
     1201 aacgcaacgc gcatttcttg gcgatttcat agccgccaag aagcaccgct gatgcgaaac
     1261 cgagtcctat ccacgacatg tcgcgattct atcacgacgt tcgcgagaca tggccgacgc
     1321 cggggattcg tggtagaatc acgtcttccg gtatgccgga gccgcggcgg taccggagag
     1381 gtcagcggtt ccattggagg gtgtatgaaa taccgggttc tggtcgcaga tgcagatctc
     1441 tccagcctga acgaaaccca gcgcgagctg acgtcgcgcg gtctgagcgt ggccgtggcc
     1501 gcgacattca acgaggcgtg tcgtctgtta cgcgaagacg gtctgattca cgtcatgctg
     1561 accgagctgt ccctccccga agacgagacg cccgatgcgc cgattatgac cggtcccgag
     1621 atgttcgaag agtttctgcg cattcgatac gaggtgaaca tttttctgtt gactgccgag
     1681 aaggcaggcc gccgtgtggt caccggcggg ttgctcaacg ggtactttta caaaggggac
     1741 cacgatttcg acgatgtgat ccgcaaggtg cgcaccgagg tgatctcgag caagaaccga
     1801 gcgcctttct tcgaaactct ggtggaatac gctgagaaag cgaaagattc ctggcacacc
     1861 cccggccacg cctcaggtga ttcgattcgc aactcggtct gggtcaagga ataccacgat
     1921 tttttcggac cgaacgtctt tcgctccgac gtgtcggtgt cggtgccgat gctcgattcg
     1981 ctcctgaatc cgaccggggt gatcaaggaa gcgcaggaac tggcggcgcg ggctttcagt
     2041 tcccgctata ccttcttttc gaccaacggc acctcgacct cgaacaagat tctgctgcag
     2101 acactgctga agcccggcga cgcgattctg cttgatcgca attgtcacaa gtccgttcat
     2161 tatggcgtga tcatcagcgg tgccgaacct atctatcttc ttccctctgc caatcagaca
     2221 tacggcattt tcggaccggt acccaaacaa cgcattctca cgaccatgga tcaagcgctc
     2281 gctgcgggga agcgggtaaa agcgctgatt ctgaccaact gcacctacga cggcctgacc
     2341 tatgatctgc cgccgatcat tgccgaggca cacaagcgtg gtatcaaagt gatcatcgat
     2401 gaggcctggt acggatacgc ccggtttcac ccggcattct atccgtgcgc gatggaggcg
     2461 ggagccgatt atgcgaccca gtcgacccac aaaaccatga gtgccttcag tcaggcatcg
     2521 atgattcacg tgaatgatcc tgatttcgaa cagattcagg attttttcta cgagaacttc
     2581 aacatgcata cctcgacgtc tccggggtat ccgatgatcg cgtcgctcga tgccgcgcgc
     2641 aagcagatgg tcatggaagg atacagcctg ctgtcacgtt gtcttgatct ggctgagagt
     2701 ttccgccagg cgatcaactc gctgcggaaa ttccgggtac tctcactcga agacatgctg
     2761 accgacgaga ttcgccatga ccatgtgcgg cttgacccga ccaagatcac gatcgattgc
     2821 tcgcgctcgg gcttcaccag caaggaagtg gagcggattc tgctgtcgaa acacaacatt
     2881 cagatcgaga aatcgacgtt taatacgctg acggtgctgc tgacgatcgg ggccacccaa
     2941 tcgaaactga cccggttgtt tctggcgctc gagaacatcg aacgcatggg gggcaacgtc
     3001 agcgatgatc cgcatctgat gccgcgcgag ttccgtctca gcctttctgc catgaagttc
     3061 atgccccgtc acgcctttta ctgtgatggc gaaaaagtga atctacgaga ggcagaaggg
     3121 aaggtttcga cggtaatggt tgtcccgtat cctccgggaa ttccgctgct ggtgcccggc
     3181 cagaccgtca caggcgagat catcaaggct ctcgaaattt accgggatca tcatgtcgag
     3241 attcatggcc tgcatgaagg catgatcaag gtcatgactc cgcctgagga gaaccggctc
     3301 gctgaacgtg gacacgcaat ttcgccgctc gaggagacac gcgcgtgaca cattttggct
     3361 tgatttcgct gctgccggcg gccgtcacca ttctggttgc ggtggtcagt caacggatcg
     3421 tcctggcgat ctttctggga atcgtggctg gatcactggg agcgaccgcg ggagcctggg
     3481 acccggcatg ggtgaaggtc gaacagtatc tttccgtctc ttttcaggat ctggaacgtc
     3541 tgaaaatcgc ggccttcgtc atcatgatgg gagccctggt cgaggtgatc gcccgtagcg
     3601 gcgcctatct caaattgtcc gaggcctttc gacggttcct caacaccccg cgcaagggac
     3661 gtctcgcctc gatcggcctg gggggaatcg tcttttttga tgattacgcg aacctgctga
     3721 tctcaggaac ctcgatgcgc ggcttgggaa ccgctttgcg tatctcaccc ggactacacg
     3781 ggtatctggt cgatcagttg gcgacggtgg tgtcgctgat gttcatttcg acctgggcgg
     3841 cttatgaagg gtcgctgatg accggggcgg ctgagatggt cggtctgaaa tccacgccga
     3901 cgcagatgct cgtcgactcg ttgccgtatc atgtgttcac gattctgggc ctgtttctgg
     3961 ccttggtggt tgcctgggag ggacgctggt ttggtgcggc gtttgatgcc gagcccgaca
     4021 aggccgtgcg tccggtcaac gtggtcggag tcggggcgcg gttgtggcat ggattgctgc
     4081 cgctgatgac gctggtcggg gtgacggtgt ggggagtgta tacgctggcc atggaagcgc
     4141 agatcaaact gggcccggcg gcgacctggg tgaatctgct ggctgaagtg ccgacgatca
     4201 acgtgctcct gttcgcgacc gcggccggtc acatggtcgc tcttctgacc ctggtgccga
     4261 gtgccgtgct gatgcccttt cagtatcgca aagcgatttt cgaagggtgc tgccacatga
     4321 ttcccgccgg gttggtgatc attctggcca agggattgtc gcttgtggca ggcgatctcg
     4381 gtaccggcac ctacataacg gagctgctga agggactcgc ggttcccgcc gtggtgccgg
     4441 cgctggtgtt tgtggtctcg tttctgattt cggtggcgac gggcttttcg tggagttcga
     4501 tggcgatcgt catgcctgtc gcgtatcaga tggccatggg agtgggcggt gcgtccatga
     4561 tcccgat
//
LOCUS       DAORHD010000215         4544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20203_length_4544_cov_3.306527, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000215 DAORHD010000000
VERSION     DAORHD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20203_length_4544_cov_3.306527"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2402
                     /locus_tag="PKO06_05885"
     CDS             <1..2402
                     /locus_tag="PKO06_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69209.1"
                     /translation="GNLEMSVGTGLYLRSFSPRRAFLFHNGMAPKNAAPLFPVVYVSV
                     QEQFWAIGHLLCRKMFLDRHPGTWAEIIDLERPLNKWRIPPDTRPSLLQLDLLTRGLS
                     DDRAWLSQGRFWRQIVMSPRYRLVFSALDTSTAKVRVTRQEWNDRILLTCVMILLGVR
                     FLMLMIGHFSLRTRLLLLFLYTTAIPLIMLGVSAQAFVSDRRENLERRTFRHAENVLR
                     KVDDSALEQLSRFERRGRAMERLDLPSGDAARDLVASLASEMARELRPTYALFSGEKG
                     KEVFQHLPDRTNRSGQDFSHQLKKITPLFTYQVARRMADLNRRELPPSESAREQAISA
                     TIESLGIDIGQFVTHMEDFTGRIYSVAFGNIKTFGYGFFIQDLSGLVRFGTWFVWVED
                     RLFQFFCDSVREFQKKVPDWCILLGYQVFPDTRSNPAPITISRILSRLEWDPLPQHAT
                     EQVGTETLLISGTWSERVVRHRLLCAQPDTPIRTDLAAAQRRLTLVASLLLLSGIIVG
                     FLLSRHLLGPVRELGQGIEAVRHRDFRVRLPVHSPDELGQLGETFNRVVEGLGELETA
                     KVVQETLFPSATLDRDAWEVSGFCRTASRIGGDYYDYFRLADGRWLLMIGDVSGHGTA
                     AALVVAMAKVVVCHSATPPDPVSILELMNRELVQTVKRRRMMTCFVAIFDPTSRVMIA
                     SNAGHSFPLKVSDRRVEEVKIEHPLLGAKIRTPFPRHEIRLLEGDSIIFYTDGLVETL
                     DRDGQQIGYDRFAAALPELMRPTATETTQAILDWRSQLAGSPALEDDVTVMALQSRRH
                     A"
     gene            complement(2473..3465)
                     /locus_tag="PKO06_05890"
     CDS             complement(2473..3465)
                     /locus_tag="PKO06_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase"
                     /protein_id="HNV69210.1"
                     /translation="MRRCSHHLFFTGLLLVLSIVRVVTALAYDPMVIPATFTPEFHDL
                     TVVDAERSREIPIRVYLPATTEPAPVAVFSHGLGGSRTNNVYMGNHWSARGFVVVFLQ
                     HPGSDESVWKNTGFGKRWKAMNEAASGQNLQLRARDVPVALDQLEVWNREKGHILFGR
                     LDMTRVGMSGHSFGAHTTQAVAGQFFPLKGQCFTLPGLRAAIMFSPSSPKVGSAEKAF
                     GQVKIPWLLMTGTRDVARIGGASLESRLAVYPALPPGSKYELVLHEAEHYAFSEREGA
                     GIGAPRNPNHHRAILAITTAFWEAYLRDDPQARAWLDGAGAGSVLEPQDRWQKK"
     gene            complement(3488..4204)
                     /locus_tag="PKO06_05895"
     CDS             complement(3488..4204)
                     /locus_tag="PKO06_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNV69211.1"
                     /translation="MKRWLKRVLLVMVVVTLGFAGYQAFEVYAARAHTVEKVIPMLAN
                     PNLKLSKTDLTARQLEILLKVEDPAFYSHNGVDLTTPGAGITTITQGLVKLLYFDKFK
                     PGIAKIKQSLIAIFALNALVSKDDQLKLFLNLAWFGVNDNQTPILGFGQAAQAFFGRD
                     LPQLSEDEFIALVGTLVAPAIFSPLKHNGQSAERVARIRKLLSGDYQPKGLFDIYYGK
                     IPAEFQKDLAPASYFESTYE"
     gene            4341..>4544
                     /locus_tag="PKO06_05900"
     CDS             4341..>4544
                     /locus_tag="PKO06_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HNV69212.1"
                     /translation="MKGLSQRQQQVLELVAGYLRDGVPPAAAQLAERLGLAGESSVTP
                     VLQALARKGFLAILGGVRGRQRTL"
BASE COUNT          884 a         1339 c         1367 g          954 t
ORIGIN      
        1 gggggaacct cgagatgagc gtcggaaccg ggctgtatct gcgctcgttt tcaccgcggc
       61 gggcttttct ctttcataac ggcatggctc cgaagaacgc ggcccccctt ttccccgtcg
      121 tgtatgtctc ggtacaggaa cagttctggg cgatcggtca tctgctgtgc cgcaaaatgt
      181 ttctcgacag acacccggga acctgggccg aaatcatcga tcttgaacga ccgttgaaca
      241 aatggcgcat accgcccgat acccgcccct ctctgctgca gctcgatctg ctgactcgcg
      301 gactctcgga cgacagagcc tggctctcac agggtcgttt ctggcgacag atcgtcatga
      361 gtccgagata ccgactcgtc ttttctgctt tagataccag tacagcgaag gtacgagtga
      421 caagacagga gtggaatgac cgcattctcc tgacgtgtgt catgatactg ctcggagtca
      481 gattcctgat gctgatgatc ggtcatttct ccctgcgcac gcgactgttg cttctgtttc
      541 tctacacgac ggccattcct ctgatcatgc tcggagtttc ggcgcaggcc ttcgtgagcg
      601 atcgccgcga aaacctcgag cggcgaacct ttcggcacgc cgagaacgtt ttgaggaagg
      661 tggacgattc ggcgctcgag cagttatcgc ggttcgagcg ccgcggtcgg gcgatggaac
      721 gactcgatct tccgtctggc gatgccgcca gggatctggt ggcctctctc gcgtcggaga
      781 tggctcgtga gctccgtcct acctatgcct tgttcagcgg cgaaaaaggc aaagaggtgt
      841 ttcagcatct gccagatcgt acaaaccgat caggacaaga cttcagtcac caactgaaaa
      901 agatcacgcc gttattcacg tatcaggtgg cacgtcgcat ggccgatctc aaccgaaggg
      961 aactgccgcc gtcggaaagc gcgcgggagc aggcgatctc cgcgaccatc gaatcgctcg
     1021 gaatcgacat cggccagttc gtcacgcata tggaggactt cacggggcgc atctactccg
     1081 tcgcgttcgg caacatcaag accttcggct atggcttctt cattcaggat ctgagtggtc
     1141 tcgttcgatt cgggacctgg tttgtctggg tcgaagatcg attgtttcag tttttctgcg
     1201 attcggtcag agagtttcag aaaaaggtgc ctgactggtg catcctccta ggataccagg
     1261 tgtttccaga tacacgttca aaccctgcgc cgatcaccat cagcaggatc ttgagccgac
     1321 tggaatggga ccccttgccg caacacgcca ccgagcaagt cggaacagag acgttgctga
     1381 tcagcggtac ctggtcggaa cgggtggtgc ggcaccgcct gctgtgcgca caacccgata
     1441 ctcccatccg taccgacctt gccgcggcac aacgacgtct gacgctcgtg gcttcgctcc
     1501 tgcttctttc cgggatcatc gtcgggtttc ttctctcacg tcacctgttg ggcccggttc
     1561 gagagttggg gcagggcatt gaagccgttc gacatcgaga tttccgtgtg cgactgccgg
     1621 tacattcgcc cgatgaactg ggacagcttg gcgaaacctt caatcgtgtc gtcgaaggtc
     1681 tgggcgaact cgaaaccgcg aaagtggttc aggaaaccct ttttccgtca gcgactcttg
     1741 accgcgatgc gtgggaagtc agcggtttct gccgtaccgc cagtcgcatc ggcggcgact
     1801 actatgacta tttccggctg gcggacgggc gctggctgct gatgatcgga gacgtttcgg
     1861 ggcatggaac cgccgccgcg ctggtggtcg ccatggccaa ggtggtcgtc tgtcactcgg
     1921 ccactccgcc ggatccggta tctattctgg aactgatgaa ccgtgaactg gtacaaacgg
     1981 tgaagcgacg ccgcatgatg acctgcttcg tggcaatctt cgatccgaca tcccgcgtga
     2041 tgatcgcctc caatgcaggt cacagttttc ccttgaaagt gagcgaccgt cgcgtcgaag
     2101 aggtgaaaat cgagcacccc ctgcttgggg cgaaaattcg cacgccgttt ccacgccacg
     2161 agattcgttt gctggaggga gattccatca ttttctacac ggacggactc gtcgaaaccc
     2221 ttgaccgcga cggccagcag atcgggtatg accgctttgc cgccgccctt cccgagttga
     2281 tgcgaccgac cgcgactgag accacgcagg cgattctcga ctggcgatca cagctggcgg
     2341 gctcgccagc cctcgaggac gacgtcaccg tcatggccct tcagtcacga cgacacgctt
     2401 gacggcgttc acctcgccgc cggcgtgaaa cccaaggggt gcgagtgccg tagaagaccc
     2461 ccgtgaccgc cattactttt tctgccagcg gtcctgcggt tcgagcaccg agccggcacc
     2521 ggcgccgtcg agccaggcgc gggcctgcgg gtcgtcgcgc aggtaggctt cccagaaggc
     2581 ggtggtgatg gcgagaatgg cgcggtgatg attcgggttg cgcggtgcgc cgatgccggc
     2641 gccctcgcgt tccgagaacg cataatgctc ggcttcgtga agtacgagtt catacttgct
     2701 tccgggcggc agggcagggt agaccgccag cctcgattcc agagatgcac cgccgatacg
     2761 ggcgacgtcg cgcgtgcccg tcatcagaag ccacggaatc ttcacctgtc cgaaggcttt
     2821 ctcagccgaa ccaaccttcg gactactcgg actgaacatg atcgcggccc gcaaaccggg
     2881 cagggtgaag cactggccct tcagcggaaa gaactgtccg gcgacggcct gcgtggtatg
     2941 cgcgccgaac gaatgtccgc tcatgccgac gcgggtcatg tcgagccggc caaataggat
     3001 atgacccttt tcgcggttcc agacttcaag ctggtcgagc gcaaccggta catctctggc
     3061 gcgtaactgc aggttctgcc cgctcgccgc ttcgttcatg gctttccagc gcttgccgaa
     3121 accggtgttc ttccagaccg attcgtcact gccggggtgc tgcagaaaca ccaccacgaa
     3181 cccgcgcgct gaccagtggt ttcccatgta gacgttgttg gtgcgcgagc cgcccaggcc
     3241 gtggctgaac accgcgacgg gggccggttc ggtggtggcc ggcagataga cgcgaatcgg
     3301 aatctcgcgt gaacgctcgg cgtcgaccac ggtcaggtca tggaactccg gcgtgaaagt
     3361 ggcgggaatg accatcggat cgtaggccag agctgtcaca actcggacga tcgacaatac
     3421 cagaagcaag ccggtgaaaa acaggtgatg tgaacaacgt ctcatcgggg cacctcgaac
     3481 gatgtgatta ttcatacgtg ctctcgaaat atgaggccgg ggcgagatct ttctgaaact
     3541 cggccgggat cttcccatag tagatgtcga acaagccttt gggctggtag tcaccgctca
     3601 gcagttttct gatgcgtgcc acccgctctg cgctctggcc gttgtgtttc agggggctga
     3661 agatcgccgg ggcaacgagg gtgccgacga gcgcgatgaa ctcgtcttca gacagctgag
     3721 gcaggtcgcg gccgaaaaaa gcctgcgcgg cctggccaaa cccgagaatc ggcgtctgat
     3781 tgtcgttcac gccgaaccag gccagattga gaaacagctt cagttgatcg tctttcgaga
     3841 ccagggcatt cagggcaaag attgcgatca gtgactgctt gatcttggcg attcccggtt
     3901 tgaacttgtc gaagtagagc agtttgacca ggccctgcgt gatcgtcgtg atgccggctc
     3961 ccggggtcgt caggtcaacc ccgttatggg aatagaaggc cgggtcttcg actttcagca
     4021 gaatctcgag ctgtcgcgcc gtcaggtcgg tcttcgaaag tttcagattc gggttggcca
     4081 gcatcggaat caccttctcg accgtgtgcg cccgtgccgc gtagacctca aaggcctgat
     4141 acccggcaaa cccgagcgtc accaccacca tgaccagcag aactcgtttc aaccagcgtt
     4201 tcatctttcc ctcacgccct tcattcgatc cgctcgatca taccgcattt ttggttgacg
     4261 gggcggcggg gttcggatat gtttagaata tcgtgatatt tcacgatact atgacaacgc
     4321 cgccgaaccg gagaaaacgc atgaaagggt tgagtcagcg acaacagcag gtgctcgagc
     4381 tggttgcggg gtatctgcgc gacggagtgc cgccggcggc ggcgcagttg gccgagcggc
     4441 tcgggctggc gggcgaatcg agcgtgacgc cggtcttgca ggccctggcc cgcaaggggt
     4501 ttctcgccat tctgggcggc gtgcgcggcc gacagcgcac cctg
//
LOCUS       DAORHD010000216         4531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20279_length_4531_cov_3.682306, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000216 DAORHD010000000
VERSION     DAORHD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20279_length_4531_cov_3.682306"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKO06_05905"
     CDS             <1..355
                     /locus_tag="PKO06_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV69213.1"
                     /translation="NVRAVVLQRFELGEPDASGRRKPVPIKGSEFEIEIDTIVIAIGQ
                     GPNPLVPQTTLDLQVNKWGNIVVKSEQTLETSKPGVYAGGDVVTGAATVILAMGAGKT
                     AAKAIHEAIQKQSAA"
     gene            486..1751
                     /locus_tag="PKO06_05910"
     CDS             486..1751
                     /locus_tag="PKO06_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69214.1"
                     /translation="MAVREGKWRCSNCRVVNRGRDLNCTGCGVVRGNVKFFLDEEAPE
                     VQDEGLAKRATDGPDWICAFCDTSNVQSATVCKQCAGARTEGKQRETAVRQDAREDAS
                     AQIRQVNLQTIRDDTPSPTPAPSTAAKAAPAAPSETPKGSAKGCCGCFATLFALFLLF
                     GLYDSLFTSTEIAEVVNRSWKRTLHLQVLVDEPWQGWEKEKPPKFRVSRSESRVSGHT
                     KTVIGYDDVEEKYQEKELAGTKTKTKTVRRKVQTGTKQVKTGVRDLGNGFFEDVYKEV
                     PVYDYENETVEEEVPVYRNVTRTRIVKKPRYRHDPINENWLEGTRQVWKKADSQTSQG
                     TFEPPAWPPVPLGTAEATAVGTYLESGREEAYQATLRGVTSGKTHEVTTIKTRPLDSA
                     LFEKLTQGSRWQLEISGLGSLDGLTPVAP"
     gene            complement(1770..2714)
                     /locus_tag="PKO06_05915"
     CDS             complement(1770..2714)
                     /locus_tag="PKO06_05915"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNV69215.1"
                     /translation="MNNLLEFPKQQRLACEYAPIIHELLRVKGIHSSDFACYNLMGWY
                     LERPPMVSRFGDQIILDVEGPGGTHLYLPPLGHGSPLPAIQAILRHQAKFGGELSIGY
                     VPEALLAQIKDDPALDRVEEQRDEFDYLYDRHELAELAGRKFHQKKNFVNRVQQSHNP
                     QVEPLTPDNLCQVMTYLDAWYAEHAAHAAVDLSTRLESLAAGRALPNLGHLGGIGLVV
                     RIAGTIEGVTVASPVHDGCWIVTLEKANREIKGLYQFINWSLANHLPDNVKLINRETD
                     LGIEGLRSAKLSYHPVGFEKKYRLYFSETFLRNECSAI"
     gene            2839..3945
                     /locus_tag="PKO06_05920"
     CDS             2839..3945
                     /locus_tag="PKO06_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HNV69216.1"
                     /translation="MATMQNSILFQPLTLRGHQLKNRLFSAPMASRYPTSKGEVTDAL
                     VSYYAHLALTGIGMTVVEGALVSTEGPGWPHELGIYHPIFQDGLARLAETIRERGSVP
                     VIQLHHAGRRGLGGRTEALIGPSALPCPAFNRPVRPMTIEEIRLMIVKFTDAALLAAK
                     AGFAGVEIHGAHGYLLHQFLTPLVNDRTDEYGVKSGNGSRFPLDVVRSIRLALPKHIV
                     GYRLSARDYLPNGLTLNETGPFAEALTQAGVDYISVSGGMYASLYGPDSIWGRKAPFA
                     VFRSDARAIKDRAAVPIGVAGKIQQTSMAVSILENRDADLIGLGRVILRDPSWIARAR
                     GEISTPISACLLCERCQYHTRGCPDTGHKPPWLL"
     gene            3967..>4531
                     /locus_tag="PKO06_05925"
     CDS             3967..>4531
                     /locus_tag="PKO06_05925"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNV69217.1"
                     /translation="MRRFFRPFVLQFLALLVFTTTAPAAGLFDLFGGMSGQTDSAVAV
                     QYVEGDKSADDEILVIKVKGLIYERGADDSDSMPWDMKKDMIETLRKDVKAALDRAAV
                     KAILLEIDSPGGEVTATDIIHHELLKIREAKKPMVALIGTMAASGGYYVACAADWIIA
                     HPTSIIGSIGVLMRAMNLEKLAAMIGYK"
BASE COUNT          985 a         1387 c         1292 g          867 t
ORIGIN      
        1 caacgtgcgc gccgtcgtgc tgcagcggtt tgagctcggc gaacccgatg cctccggccg
       61 ccgcaaacca gttccgatca agggttccga gttcgagatc gaaatcgaca ccatcgtcat
      121 cgcgatcggt cagggcccga acccgctcgt gccgcagacc acgctcgacc tgcaggtgaa
      181 caagtggggc aacatcgtgg tcaagagcga gcagacgctc gagacctcaa agcccggcgt
      241 ctatgccggc ggcgacgtcg tcaccggcgc cgcgaccgtc atcctcgcca tgggcgccgg
      301 caaaaccgct gccaaagcca tccacgaagc cattcagaaa cagtccgccg cctagttcgc
      361 ggtctccagg ccgggtgcac gaacgatgca cccggctttt tctttttccc tgtccttcat
      421 ctcgcgcgac gaatcgctgt gatattattc gtggagcgag cgcatcatac cagcaggaga
      481 aacagatggc ggttcgggaa ggcaagtggc gttgttccaa ctgtcgtgtc gtcaatcgcg
      541 gacgtgatct gaactgcacc gggtgcggag tggtacgcgg caacgtgaaa ttctttctgg
      601 atgaggaagc acccgaggtc caggacgagg gattggcaaa gcgcgccacc gacggccccg
      661 actggatctg cgcgttctgc gacaccagta atgtccagtc cgcgacggtc tgcaaacagt
      721 gcgccggagc ccgcaccgag gggaagcaac gtgaaaccgc cgtgcgacaa gatgcccgcg
      781 aagacgcctc ggctcagatc cgccaggtga accttcagac cattcgcgac gacactccgt
      841 cgccgacgcc ggctccatca accgcagcga aagcggctcc cgcggctcct tccgagactc
      901 ccaaagggtc cgcaaaaggg tgttgcggct gcttcgccac tctgttcgct ttgttcctgc
      961 tgttcggcct gtacgattcg ctattcacga gcaccgaaat cgccgaagtg gtcaaccgct
     1021 cctggaagcg gacgctgcat ctacaggtcc tggtcgacga accctggcaa ggctgggaaa
     1081 aagagaaacc gcccaagttt cgtgtctctc gttcagaatc acgcgtttcc ggacatacga
     1141 aaaccgtcat cggctacgat gatgtggaag agaagtatca ggaaaaggaa ctcgccggca
     1201 ccaaaaccaa gaccaaaacc gttcgtcgaa aagttcagac tgggacaaaa caggtcaaga
     1261 ccggcgtgcg tgatctcggc aacggctttt tcgaagacgt ctacaaggaa gtgccggtct
     1321 atgactacga gaatgaaacc gtcgaggaag aggtgccggt ctaccgcaac gtgaccagga
     1381 cccgcatcgt caagaagccg cgataccgac atgacccgat caacgaaaac tggctcgaag
     1441 ggacacggca ggtgtggaag aaagctgact cacagaccag ccagggcacg ttcgaacctc
     1501 ccgcgtggcc accggttccg ctcggtactg cagaggcgac tgccgttggc acgtatctgg
     1561 aaagcggccg cgaagaagcc tatcaagcaa cactgcgggg agtcacatcc gggaaaaccc
     1621 acgaggtcac gaccatcaaa acgcgaccgc tcgattcagc cctgttcgaa aaactgactc
     1681 agggaagccg ctggcaactc gagatcagcg gactcggcag cctcgacggg ctcacccctg
     1741 tcgcaccctg acgcccaagg aagtccaatt cagattgcgc tacactcgtt cctgagaaat
     1801 gtttccgaga aatacaaccg gtactttttc tcgaagccaa cggggtggta actgagcttc
     1861 gccgaacgta gcccctcgat gccgagatcg gtttcacggt tgatgagctt cacgttgtca
     1921 ggcagatgat tcgccaatga ccagttgatg aactgataca atcccttgat ctcacggttg
     1981 gctttttcca gggtcacgat ccagcacccg tcatgaacag gcgaggcgac ggtcacacct
     2041 tcgatggtgc ccgcaatgcg caccacaagc ccgatgccac ccaggtgccc gaggtttggc
     2101 aacgcccgtc cggcagccag tgattcgagt cgggtcgaaa gatcgacagc cgcatgtgcc
     2161 gcatgctcgg cataccaggc gtcgagatag gtcatgacct gacacaggtt gtcgggggtc
     2221 aggggctcaa cctgcggatt gtggctctgc tgaacccggt tgacgaagtt ctttttctga
     2281 tggaacttac gaccggccag ttccgccagt tcatgtcgat cgtacaagta atcaaactca
     2341 tcccgctgtt cctcgacacg gtcaagagcg ggatcgtcct tgatttgtgc caacagcgcc
     2401 tcagggacgt atccgatgct gagttctccc ccgaactttg cctgatggcg aagaatcgcc
     2461 tgaatcgcgg gaagcggcga accgtgaccg agcggcggca ggtagaggtg cgtccccccc
     2521 ggcccctcga catcgagaat gatctggtca ccgaatcggc tgaccatggg cggacgctcg
     2581 agataccaac ccatcaggtt atagcaggcg aaatcagacg agtgaatgcc tttcacccgc
     2641 agcaactcat gaattatcgg ggcatactcg caggcgagcc gctgttgctt tggaaattcg
     2701 agcaggttgt tcatatcggc ggattgtatc acaactgccg ctgatccgcc atggatcaac
     2761 gagaacagtc gacctgagcc gactgagagg catggacgac gcagtccgaa gttgcctttt
     2821 ccgcatccag tgtctatcat ggcgacaatg cagaattcca ttctctttca gccgctcact
     2881 ttacgcggtc atcaactcaa gaaccgcctc ttctccgctc cgatggcaag tcggtatccc
     2941 acctcgaagg gcgaggtgac cgacgccctc gtctcatact acgctcatct ggcgctgacc
     3001 ggcatcggca tgaccgtcgt cgaaggcgca ctggtctcga ctgaagggcc cgggtggcca
     3061 catgagctgg gaatctatca tcccatcttt caggacggtc tggcccgtct cgccgagacc
     3121 attcgagaac gcggttcggt cccggtcatt cagctgcatc acgccggtcg tcgcggactt
     3181 gggggccgca ccgaagctct gatcgggcct tccgccctcc cctgccccgc cttcaatcgt
     3241 ccagtgcgcc ccatgaccat cgaggaaatc cgactcatga tcgtgaagtt caccgatgcc
     3301 gccttactgg ccgccaaagc cggtttcgcg ggcgtcgaga ttcacggcgc acatgggtat
     3361 ctgctgcacc agtttctcac gcccctggtg aacgatcgca ccgacgaata cggcgtgaag
     3421 tcgggcaacg gcagcagatt tcccctcgat gtcgtccgtt cgatccgcct ggccctgccc
     3481 aagcatatcg tcgggtatcg actttcggct cgagattatc tgcccaatgg cctgaccctg
     3541 aatgaaaccg ggccgtttgc ggaagcattg acgcaggccg gggttgacta catcagcgtt
     3601 tccggcggca tgtatgcgtc gctctatggt cccgacagta tctgggggcg aaaggctccg
     3661 ttcgcggtct ttcgcagcga cgcccgggcg atcaaggatc gagcggccgt tccaataggg
     3721 gttgccggca agattcaaca gaccagcatg gccgtctcga ttctcgaaaa ccgcgatgcg
     3781 gacctcatcg gactcggccg cgtaattctg cgcgatcctt cctggatcgc ccgcgcgcgc
     3841 ggtgagatct caacgcccat atcggcctgc ctgttgtgcg aacgctgtca atatcacacc
     3901 cgcggctgcc cggataccgg gcacaagccg ccctggttac tctagaaatc acccgaggag
     3961 atatccatgc gaagattctt ccgacctttc gttcttcagt ttctggcact gctcgtgttc
     4021 acgactacgg ctcccgcggc cggattgttc gacctgttcg gcggcatgtc gggacaaact
     4081 gattcggcgg ttgccgtgca gtatgtcgaa ggcgacaaga gcgcagatga tgagattctc
     4141 gtcatcaagg tcaagggatt gatctacgaa cgcggggctg acgactcgga ctccatgccc
     4201 tgggacatga agaaagacat gatcgaaacg ttgcggaagg acgtgaaagc ggctctggac
     4261 cgcgccgcgg tcaaggccat tctgctcgag atcgattcgc ccggcgggga agtcacggcg
     4321 accgacatca ttcatcacga actgctcaaa atccgagaag ccaagaagcc aatggtggct
     4381 ctcatcggca cgatggccgc ttcgggcggg tactatgtcg cttgcgccgc cgactggatc
     4441 atcgcccatc cgacctcgat catcggcagt atcggcgtgc tgatgcgcgc catgaacctc
     4501 gaaaaactgg ccgccatgat cggatacaag g
//
LOCUS       DAORHD010000217         4513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20405_length_4513_cov_3.799686, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000217 DAORHD010000000
VERSION     DAORHD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20405_length_4513_cov_3.799686"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKO06_05930"
     CDS             complement(<1..540)
                     /locus_tag="PKO06_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69218.1"
                     /translation="MNDQIPFLHSLKFKFFLGFALLVGLIVLLASVNSSVREEALLSD
                     SMNQAGTLLSSTLALACQEPIIKQEFDIFGQYCERIIQTGDDIMEVAVVDQDGKYLAH
                     RRRNDTNSLLGTSLEAAEKQILEAITGPQKLACPDRNCIQYFAPIRVGSSVLGMVILR
                     YSFERLRQAQASNRARIAGI"
     gene            complement(550..1518)
                     /locus_tag="PKO06_05935"
     CDS             complement(550..1518)
                     /locus_tag="PKO06_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV69219.1"
                     /translation="MKHQNALVRLITMLSLITAVWLTACGGGPTPPAQPQPTPTPPTT
                     GNTASTGGAMKDLVLKLGRIPFTNSSGLMRASEELLKYLSQELGVKEVRLITGSDYHS
                     LITKLEREEIDIGWLGSLSYVEAAQRLKLKPLVKPTRFDTNSYRGLIITRQDSGIKSL
                     ADLKGKKFAWVEKESASGYTFPKALLIEAGINPDKDFAEAAFLQKHDAVVLNVLIGKY
                     DAGACYDDARNTLRDKEKINDLTVLARTEDISNEPIVCRAGLPEDLVEKIRKAFLKLD
                     FNNPAHKKVLDELTDVRGFVPASDADYNYVRKVCELLTTHDGAKPQ"
     gene            complement(1617..3077)
                     /locus_tag="PKO06_05940"
     CDS             complement(1617..3077)
                     /locus_tag="PKO06_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69220.1"
                     /translation="MTHFVPLRSFRLLGLAVIICLSVLLPAWVATLHADPVASATAES
                     TPTSKATYDCQYLVELKKAGDFLELLPQHPREVLRRLGKKLQPFLGQFTTPTLEFAGF
                     TPGEKVTNTPLGDPARISQWNDQQRELVSQLRTRTSALLNDLEREVGVRPSGVSLRFA
                     ADPKTWLGPLMKMMDNPEATKNLRANQLFNLRTKLLPLLETADLIIAAATISDTGLDF
                     IIRALGSREFTASVSEAYTPDKTLSCARFLDEQHLCTFGQVHVLPSPQIILQHLKTIP
                     QLSIVENFLKNAGLDLEKDILPAQAVDSLVSINLDPTGDGGLPDFRVIARFADPMKIL
                     AILPGLSKLCTDLGIYVASQTEMPVGVKLSYFLVPTFGLHLALTDGHVLLATSRANLV
                     NLHQHFQNVITGKKPAFTVPEGSHRYWRIRFKNLNEQIQRFLQSPLLAGKGIPPIPNL
                     SMLEELGDFTMTTRMKPEYLEVRLTLPIVTQSDKAK"
     gene            3224..>4513
                     /locus_tag="PKO06_05945"
     CDS             3224..>4513
                     /locus_tag="PKO06_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69221.1"
                     /translation="MSCFTGSRQSGFVLFIVIAVLLAIAIMAISLSSFKSGAVELLGK
                     TMDQNRLATLAQSANHEVIGMVKTQVNRLGTDPHNRFRQFFQPGGVGSAYSPVDQSSL
                     PKPVVIYDDIQPPATKALADAFAGLGINIKSRAVLTVTAQRNLKGYPSYVGHLEVTSR
                     AYYGGKYNNSVEVKERRDVKMIDVHDLFDKYALFVKNYSPDYNNPNRRLIVEGISPQD
                     WANEVYSRVYLGSRFYPESKEFESTGATGAIYLDLNLKDYGAVVGRLLNSAVAPTAQN
                     FTWANPSSKPGSVNELPMIWVSPTQAFSGIFTANGYPKEDFYKTHEIMKQYEYIVHAA
                     ACAACGDPGNPPAELANGNAPRRGKKLLEKADEAWSKIDANVYTTHASVRLIIDDFRN
                     GMNSKRTLGDDALTDYSGCVGFQQVLQTHIDNWPYCFG"
BASE COUNT          996 a         1189 c         1337 g          991 t
ORIGIN      
        1 gattccggca atacgagcgc gattggaagc ctgcgcctga cggagacgct cgaacgagta
       61 gcgcagaatg accatgccga gaaccgagga accgacgcga atcggagcga aatactgaat
      121 gcagttgcga tccggacacg cgagtttctg tgggccggtg atggcttcaa gaatctgctt
      181 ttcggctgcc tcgagactcg tgccgagaag actgttggta tcgttgcgtc gacgatgggc
      241 gagatacttt ccgtcctggt caacgacggc gacttccatg atgtcatcgc cggtctggat
      301 gattcgttcg cagtactgac cgaaaatgtc aaactcctgc ttgatgatcg gctcctggca
      361 ggcaagtgcc agagtcgagg aaagcagggt gccggcttga ttcatgctgt cggacagcag
      421 ggcctcttcg cgtacggaag agttcacgga ggccagcagg acaatcaggc cgacgagcag
      481 cgcaaatcca agaaagaact tgaacttgag cgaatgcaga aacggaatct ggtcgttcac
      541 aacatgtgcc tattgaggct ttgctccatc gtgggtggtg agcagttcgc agactttgcg
      601 aacgtaattg taatctgcat cggaggcagg cacgaaacca cgcacgtcgg tcaattcgtc
      661 cagcaccttc ttgtgcgcag ggttgttgaa atccagcttc agaaatgctt tgcggatctt
      721 ttcgacgaga tcttccggga gtccggcgcg gcagacgatc ggctcattgg agatgtcttc
      781 ggtgcgggcc agcacggtca ggtcgttgat cttttccttg tcgcgcaggg tgttgcgcgc
      841 atcgtcgtaa caggctccgg cgtcatactt cccgatgagc acgttgagca cgacggcatc
      901 gtgcttctgc agaaacgcgg cttcggcgaa gtccttgtcg ggattgatgc cagcctcgat
      961 cagaagcgct ttgggaaacg tatacccgga ggcggactct ttctcgaccc aggcaaactt
     1021 cttgcccttc agatcagcga gactcttgat gccgctgtcc tgacgggtga tgatcaagcc
     1081 acggtaactg ttggtgtcga atcgggtcgg cttgaccagt ggcttcagct tcagacgttg
     1141 ggccgcctcg acatagctca ggctgccgag ccagccgata tcgatctctt cacgttcgag
     1201 cttggtgatc agactgtgat agtccgagcc ggtgatcaag cgaacctctt tcacgcccag
     1261 ttcctgagac aggtatttca agagctcttc ggaagcccgc atcagaccgg atgagttggt
     1321 gaacggaatc cgcccgagtt tcagaacgag atccttcatc gctccacccg tggaggcagt
     1381 gtttccggtg gtcgggggag tcggggtagg ttgcggctgc gccgggggtg tgggaccgcc
     1441 gccacaggcg gtgagccaca cggccgtgat aagcgacaac attgtgatca gccgtacgag
     1501 agcgttctga tgtttcacgg aatgcctctc ctcttacgaa tggatacttg ggaatgtata
     1561 acacattctc cgaatggatg tcacgaagtc ctgccgtgat tccctggcga acctggtcac
     1621 ttcgctttgt ctgattgcgt gacgatcggc agtgtgagcc ggacttcgag atactctggt
     1681 ttcatgcgtg tcgtcatggt gaagtcgccg agttcctcga gcatgctgag gttgggaatc
     1741 ggcggtatac ctttgccagc gagcaggggc gactgcagaa accgctgaat ctgttcgttc
     1801 aggttcttga aacgaatgcg ccagtaccgg tgagaacctt ctggaactgt gaatgcaggc
     1861 tttttcccgg taatgacatt ctgaaaatgc tggtgcaggt tgaccaggtt ggcacgactg
     1921 gtcgccagga gcacatggcc gtcggtcagt gccagatgaa gtccgaaagt cggcaccaga
     1981 aaataactca acttcacgcc cacgggcatc tccgtctgcg aggcgacgta gatgccgagg
     2041 tcggtgcaca gtttcgacag acccggcaga atcgccagaa tcttcatcgg atcagcgaat
     2101 cgcgcgatga cgcgaaaatc gggcaatccg ccgtctccgg tcggatcaag gttgatggag
     2161 acgagagaat cgacggcttg cgccggaaga atgtcttttt ccagatcgag accggcgttt
     2221 ttcagaaagt tttcgacgat actgagctgc ggtatcgtct tcaggtgctg cagaatgatc
     2281 tggggcgagg ggagtacatg cacctgtccg aaggtgcaga gatgctgctc gtcgagaaac
     2341 cgcgcacaac tcaaggtttt gtcaggagtg taggcttctg acaccgaggc ggtgaactcc
     2401 ctgctgccga gagcgcgaat aatgaaatcc agtccagtat cggagatggt cgctgcggcg
     2461 atgatcagat cagcggtttc gagcaatgga agcaatttcg tgcgtagatt gaacagctga
     2521 ttggcgcgaa gatttttcgt cgcttcggga ttgtccatca ttttcatcag cggcccaagc
     2581 caggtcttgg ggtcagcggc aaaccgaagg ctgacacccg aaggccttac gccgacttcg
     2641 cgttcaaggt cattcagaag cgcggaggtg cgggtgcgaa gttgcgagac caactcgcgt
     2701 tgctgatcgt tccactgtga aatccgcgcg ggatcaccaa gcggggtgtt cgtcaccttc
     2761 tcgcccggag tgaaaccggc aaactcgagc gtcggggtgg tgaactgccc gaggaacggc
     2821 tgcagtttct ttcccagccg acgcagtact tcacgaggat gctgcgggag aagttcgaga
     2881 aagtcacccg cctttttcag ctcgaccaga tactgacagt catacgtggc tttgctcgtg
     2941 ggagtggatt ccgctgtcgc actggccacc gggtcggcgt gcagagtggc gacccatgcc
     3001 ggcagcagga cgctcaggca gatgatgacc gccagaccga gcagacgaaa cgaacgcagg
     3061 ggaacgaagt gtgtcatacg gggtcctccg aatgtggcgt ctgatttcac tcgccaagta
     3121 tagcgtaccg cacctgacct gcgcgactct cgccgcttcg agccgttgta cggtgtgatc
     3181 ggatttggga atctctgcca gctgtgatac catgaacgat gtgatgagtt gtttcacagg
     3241 atctcgacag agcggattcg tgttgttcat cgtcatcgcc gtcttactgg cgattgcgat
     3301 catggccatt tctctgagtt cgttcaagag cggtgccgtc gaactgctgg gaaaaaccat
     3361 ggatcagaac cgcctggcaa ctctggctca gtcggcaaac cacgaagtga tcggcatggt
     3421 gaaaacccag gtgaaccgcc tgggtaccga tccgcataat cggttccgtc agtttttcca
     3481 gccgggcgga gtcggctctg cctacagccc cgtcgatcag tcgagcctgc cgaagccggt
     3541 cgtcatctac gacgacatcc agcctccggc gaccaaagcg ttggctgacg ccttcgctgg
     3601 cctcggcatc aacatcaaga gtcgggctgt tctgacggtc accgctcaac ggaacctcaa
     3661 agggtatccg tcctatgtgg gtcacctcga agtgacgtcg agagcctatt atgggggcaa
     3721 gtacaacaac tcggtcgagg tgaaggaacg tcgagatgtg aagatgatcg acgtgcacga
     3781 cctgttcgac aagtacgcgc tgttcgtcaa aaactacagc cctgattaca acaatccgaa
     3841 tcgacgtctg atcgtcgaag gcatctcgcc gcaggattgg gcgaacgagg tctactcacg
     3901 cgtctacctc ggcagccggt tttacccgga atccaaggag tttgagtcga caggggccac
     3961 cggtgcgatc tacctcgacc tcaatctgaa agactacgga gcggtcgtcg gaagactgct
     4021 caactccgcg gtagcaccga cggcgcagaa tttcacgtgg gcgaatccct catcaaaacc
     4081 cggctcggtc aatgaattgc cgatgatctg ggtttctcca actcaggcgt tttccggcat
     4141 ttttacggca aacgggtacc caaaagaaga cttctacaag acccatgaga tcatgaagca
     4201 gtacgagtac attgttcatg cagccgcctg tgctgcctgc ggtgatccgg gaaacccgcc
     4261 ggctgaactt gccaacggga atgctccccg tcgtggaaag aaactgttag aaaaggctga
     4321 tgaagcatgg agcaaaattg acgcgaatgt atacacgact catgcttctg tgcgcctcat
     4381 tattgatgat tttcgcaacg gaatgaacag caagcgaacg ctgggtgacg atgctcttac
     4441 ggattacagt ggatgcgttg gctttcagca ggttcttcag acccacatcg ataactggcc
     4501 ttactgtttc gga
//
LOCUS       DAORHD010000218         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20429_length_4510_cov_3.655443, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000218 DAORHD010000000
VERSION     DAORHD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20429_length_4510_cov_3.655443"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PKO06_05950"
     CDS             complement(<1..456)
                     /locus_tag="PKO06_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69222.1"
                     /translation="MMHHTPARIFRKLVLGSLLAGCVIPSLPPAPVCAQVYDNTNVLI
                     ESAVNHIYQKRYDKAHEALKKAYEQAPRHPSVHFNLGRLFELTGNFSEAQKEYQLAAM
                     LDPTMIAARRGIARVAVELKRQGSMEVRAVLDQAAREPKAVEVPRTGFHV"
     gene            complement(683..2158)
                     /locus_tag="PKO06_05955"
     CDS             complement(683..2158)
                     /locus_tag="PKO06_05955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69223.1"
                     /translation="MQTSTDYKARIARQFGFALKDPNPNIRFIAIEGLQLLPDVPELE
                     GIPALMNDTDRFVRWKATQACGLLGLESSVAALIKFLQSPDFHTRVYAAQSLGLIGTP
                     ECLEALTQAARAEGSPKVRQAITATFGHFPSSMPWDLMFRTAHDADVGVRLETAKALG
                     KAVPNEQACQILITMLEQETNNQVFATAVHSLGQFKKSSLVSYFEHCLAHQEPRIRAN
                     AIEALGCFPLPTVEKVILPYLNDPANRVKANVVHVFLANNQPGKVEKNLVGLLVSPRQ
                     FERASGAWLAGTFRLTDTQPILIKLLSDEEGIVAERAAWALGRIKAPDTFSALQTAYA
                     NANQWALAHIIKAMSEVVGAAEAPTIARLMSKEHSPLHRSQFLDILTDLAYTSALSEA
                     LKYRSDADHRLRLSAFRFIGLHGGAESRDILFQGLGEPNPKVRALCADLMIRNGDFRA
                     LKTLSELLNEQDKMQRLQAAHTLKEMAVLTKRDVAEPSKPG"
     gene            complement(2260..2772)
                     /gene="dcd"
                     /locus_tag="PKO06_05960"
     CDS             complement(2260..2772)
                     /gene="dcd"
                     /locus_tag="PKO06_05960"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HNV69224.1"
                     /translation="MAILTREELIREIRAGSIEIDPLLEDCIGPGSIDLHLGEEFRVF
                     KKLHTIYHVTDSSDFNEITELIRVKDHFVLLPGETVLGITVERIKLPPYLCGWLEGRS
                     KFARLGLMVHITAGFMQPGINNRQVLEISNVSSIPLALHPGTRMCQFIFQTTKGEARY
                     QGTFVNQMTP"
     gene            complement(2852..4066)
                     /locus_tag="PKO06_05965"
     CDS             complement(2852..4066)
                     /locus_tag="PKO06_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV69225.1"
                     /translation="MSVTIRTSNSVSPLFFLLFGIPFGGVGLLLFLLGISQLSVYYDS
                     AQWVKTPAQFEQIEVKSRRGSKGGTFHYLSVTYTYDVLGKSYQGHDLFFEDVSNRDPQ
                     PIEANANRIKAGLERDHSFPVLVNPKNPEQAVVFREFLPEMIMLPMVGGIFGAAGLGV
                     MLFGLYSFLHERKKNKALEANPQRPWKAEEQWKGFTVISHSYGQLLVTWGIALFMNLF
                     ISIFVVALATDPTAPLIAKMVIGLFGLVAVGLLCYAVYLTLQYLKYGNSSLQLSQMPL
                     VTGQDFSAVIIVGRHLEMPKGCTVTFKCRRALTTGSGKHAHTTTTELHTDTQIIQQDL
                     FRPEKGRSAIPVSFKIPPHLPPRDVESNPKFTWVLEVAAETPGIDFTTEFDLPVYHVA
                     DLSLVERRDGGF"
     gene            complement(4255..4434)
                     /locus_tag="PKO06_05970"
     CDS             complement(4255..4434)
                     /locus_tag="PKO06_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69226.1"
                     /translation="MTKNPLNEFILDKLAAGETFSQLTTKYGITRQEFINAAVYGISE
                     LRGEYISLLSKRRAH"
BASE COUNT          934 a         1205 c         1368 g         1003 t
ORIGIN      
        1 cacgtggaac ccggtgcgcg gcacctcgac ggctttgggt tcgcgggcgg cctgatcgag
       61 caccgcgcgc acttccatcg acccctggcg tttgagttcg acggcgacac gggcgatgcc
      121 gcgtcgcgca gcaatcattg tcggatcgag catggcggca agctggtact ctttctgcgc
      181 ttccgaaaag ttcccggtga gttcgaacag acggccgaga ttaaaatgca cgctggggtg
      241 ccggggagcc tgttcatacg cttttttcag cgcttcatgc gccttgtcgt agcgtttctg
      301 atagatatga ttgaccgccg attcgatcag cacgttggtg ttgtcgtaaa cttgggcgca
      361 gacgggcgca gggggcaggc tgggaatgac acaaccggcc agaagagagc ccagaacgag
      421 tttgcggaag atgcgggcgg gggtgtggtg catcatggga cacttcctgc gtgaattttg
      481 acccggccgg gtctctatgc tatgatacca ccgtacgacg atatcaccga tggggagaag
      541 aacatgtcga tttttcggtt catctggtgg gcgatcatca tcagcgccat cgcatttgcc
      601 gggtatgtgt acggatatct ttctgattga ttgatcatgt gtgatgcgcc cctgacggcc
      661 tcggccgtcg ggggcgtttc atttagccgg gtttggatgg ctcggccaca tcgcgttttg
      721 tgagaaccgc catttccttg agcgtgtgag ccgcctgcag acgctgcatc ttgtcctgct
      781 cgttcagcag ttccgaaagc gttttcaacg cccgaaaatc gccattgcga atcatcagat
      841 cagcgcacag agcgcgcacc ttcgggtttg gttcgccgag accctgaaac agaatgtcgc
      901 ggctctcagc gcccccgtga agaccgataa agcgaaaagc gctgagtcgc agacggtgat
      961 cggcatctga tcggtatttc agagcttccg acaacgccga tgtataggcc agatcagtca
     1021 gaatatcgag aaactgcgag cgatgcagcg ggctgtgttc cttgctcatc agcctggcga
     1081 tggtcggggc ttcggctgcg cccaccacct cggacattgc tttgatgatg tgcgccagag
     1141 cccactgatt ggcattcgca taggcggtct gcagagcgct gaaggtatca ggagccttga
     1201 tgcgtccgag tgcccaggcg gcccgttcgg cgacgatgcc ctcttcatcg ctcagcagct
     1261 tgatcagaat cggttgggtg tccgtcagtc ggaatgtacc agcgagccag gcgccgctcg
     1321 cgcgctcaaa ctgccgcggt gagacgagca acccgaccag atttttttcg accttgccgg
     1381 gctggttgtt ggcaagaaac acgtgcacga cgttggcttt gacacgattg gccgggtcgt
     1441 tcagatacgg cagaatcacc ttctcgaccg tcggcagcgg aaagcagccc agcgcttcga
     1501 tggcgttcgc ccgaatgcgc ggttcctggt gcgccagaca atgttcgaaa tagcttacca
     1561 gggaagattt tttaaactgt ccgagcgagt gaaccgcggt cgcgaacacc tgattattgg
     1621 tttcctgttc gagcatggtg atcaggatct gacacgcttg ttcattggga accgctttgc
     1681 cgagcgcctt cgcggtctcg aggcgcacgc cgacatcggc atcgtgagcg gtgcggaaca
     1741 tgagatccca gggcatcgaa ctgggaaaat gtccgaacgt ggcggtgatc gcctgacgaa
     1801 ctttgggtga tccttccgcc cgggcggcct gtgtcagtgc ttcgagacat tcgggcgtac
     1861 cgatcagccc gagcgactga gctgcataca cgcgggtgtg aaagtcggga gattgaagaa
     1921 acttgatcag cgcggcaacg gaagactcca atccgagaag accgcaggcc tgtgtggctt
     1981 tccagcgaac gaaccggtcg gtatcgttca tcagcgccgg aattccttcg agttcgggta
     2041 catcgggcag caactgcaat ccttcaatcg cgatgaaacg gatgttgggg ttgggatctt
     2101 tcagtgcaaa cccgaactgg cgggcgattc gagctttgta gtccgtactg gtctgcatat
     2161 gtcaccctcc agagcaaaca cacgaatgat tgtcactgtt atcgacaggt tgccgcgttt
     2221 cgataacggt atccacaggg ctcggcggcg agcgacggtt cagggggtca tctgattgac
     2281 gaacgtgccc tggtagcggg cttccccttt tgtggtctgg aagatgaact ggcacatacg
     2341 cgtgccgggg tgcagtgcca gcggaatcga gctgacattg ctgatttcga gcacctggcg
     2401 attgttgata cccggttgca tgaagccggc ggtgatgtgc accatgagtc ccagtcgggc
     2461 gaacttgctg cgcccttcca gccagccgca cagatacggc ggaagcttga tgcgttcgac
     2521 ggtgatgccc aggaccgttt cgccgggcag caggacgaag tggtctttga ccctgatcaa
     2581 ttctgtgatc tcattgaagt ctgaggagtc ggtgacgtga tagatcgtgt gcagcttctt
     2641 gaagacgcga aactcctcac ccagatgcag gtcgatcgac cccgggccga tacagtcctc
     2701 gagcagtggg tcgatttcga ttgatccggc acgaatctcg cggatcaatt cttcgcgggt
     2761 caggatggcc atgacataag actcctcaaa tggaattggc gggtattata ctcgattcac
     2821 cggttctcgc gagaacgacg gacagagtcg atcagaagcc gccgtcgcgc cgctcgacca
     2881 gcgacagatc ggcgacgtgg tagaccggca ggtcgaactc ggtcgtgaaa tcgatgcccg
     2941 gggtttcggc cgcaacttcg agcacccagg tgaactttgg attggattcc acatcgcgag
     3001 gtggaaggtg cggcgggatc ttgaacgaca cgggaatggc cgatcgtccc ttctcggggc
     3061 ggaacagatc ctgctgaatg atctgagtgt ccgtgtgcag ttcggtcgtg gtggtgtgag
     3121 catgctttcc ggaaccggtg gtgagcgcgc gcctgcactt gaatgtcacc gtgcacccct
     3181 tgggcatttc gaggtgtctg ccgacgatga tgacggccga aaagtcctgg ccggtgacca
     3241 gtggcatctg cgacagctgc agagaagagt tgccatactt cagatattgc agtgtcagat
     3301 agacggcgta gcacagaagc ccgacggcga cgagaccgaa caggccgatc accatcttgg
     3361 caatcagtgg cgcggtggga tctgtcgcga gagcgacgac gaaaatcgag atgaaaagat
     3421 tcatgaagag tgcaattccc cacgtgacca gcagttggcc atagctgtgc gagatgacgg
     3481 tgaagccttt ccactgctcc tcggccttcc aggggcgctg cggattcgcc tcgagagcct
     3541 tatttttctt gcgttcatgc aaaaagctat acaggccgaa caacatcacg ccgagtccgg
     3601 ctgcaccgaa gatgccgccg accatcggca acatgatcat ttcaggcaga aactcgcgga
     3661 acacgactgc ctgttccgga ttcttcggat tgaccaggac cggaaacgaa tggtcgcgtt
     3721 cgagacctgc tttgatgcgg ttggcgtttg cctcgatagg ctgcgggtca cgattcgaga
     3781 cgtcctcgaa gaacaggtca tgaccttgat aggactttcc caggacatcg taggtatagg
     3841 tgacgctgag gtagtggaaa gtgccgccct ttgagcctcg ccgcgacttc acttcaatct
     3901 gttcaaactg tgcgggggtt ttcacccact gtgcggagtc atagtagacg ctcagttggc
     3961 tgatgcccag caagaacagc aatagtccga cgcctccaaa cggaattccg aacagcagaa
     4021 aaaacagggg cgatacggaa ttggacgttc gtatggtgac tgacatgtcg cgagtcctct
     4081 ttcatttctg acaataacag ctaccggctc ttcatccggt gctcctattc gctgccgaca
     4141 cgttaccaca ggcagagtgt ccctgtcaca gccttgcctt gctcaacctg ggaaaaaaac
     4201 gggaccaggc gcgttggcct agtcccgtgg ttcggtcggt tccggagtgg tgagctaatg
     4261 cgccctccgc ttgctgagaa gcgaaatata ctcgccacgc agttcgctga taccgtagac
     4321 tgcagcgttg atgaactcct gacgggtgat gccgtatttg gtggtcagct gcgagaacgt
     4381 ttctccggcg gccagcttgt cgagaatgaa ctcattgagc gggtttttgg tcatgggggg
     4441 tccctcacgt catatggaat actggttcat ctatcggcag attcccgtga ttttctcaag
     4501 acttgttgtt
//
LOCUS       DAORHD010000219         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20449_length_4507_cov_4.215184, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000219 DAORHD010000000
VERSION     DAORHD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20449_length_4507_cov_4.215184"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2125)
                     /locus_tag="PKO06_05975"
     CDS             complement(<1..2125)
                     /locus_tag="PKO06_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69227.1"
                     /translation="MNIVELSIKRPVMMTMVLLFLIVLGLFSYGKIPIDMLPKIDFPM
                     VTVVTAYPGASPKNIETLISQKIEDAVSAINGIDELKSDSYEGFSNVLIRFEDYIDVD
                     TAAADVREKVGAIRDKLPDDAKEPVILKLDINALPILSLAISGNRPVESTYALAKDLI
                     RDEIRRVDGVADLDFIGAREREITIQIDQGIAKAFQISPISLAGMIAQKSLNIPSGHI
                     TQTRKEYNIRMDGEFTSVREIRELPIPTAGGKTYTLGDIAQVTDEYEEFRQAVRLNDR
                     PCIAMIVKKRSDANSVRTAEGVLKRLEELKSKLPRDIEFTPVRNRATFIVDSVDDLNG
                     NLIAGIIITAIILFLFLHSFKATLVSVLSIPISIVATYTLVYAFGFSLNMMSLMALAI
                     SVGILVNNAIIVLENIYVHLQKSKQSPKEAAITGTNEILVAVAGCTLTNVLVFTPIAF
                     MEGMVGRFFYEFGLTVTFATFVSLLVAFTLTPMCAGFFLTADDADPDKKYRFGRWWDR
                     AFTRLADDYRSLLTLIMKHRLLTFLVCGSLFGASFLLAPFIGFEFVTEPDQKEFDITV
                     KLPPGSSIDNTDRALKKIEDVLRGRPEIRFSFTKLGKTESLVGGSTTGTHLGEISVRL
                     HDTVTISTDQFIAAITPQLSQIPEVELNVRKTGLLGTSESPIMIDILGDDLEVLQRIE
                     SQMLAIVKRTPGTLDVLSSLQTGKPE"
     gene            complement(2152..3417)
                     /locus_tag="PKO06_05980"
     CDS             complement(2152..3417)
                     /locus_tag="PKO06_05980"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV69228.1"
                     /translation="MKKYLILGIVLVAAIGFGVTARLSAIRNTPKALNVSDYQTRKGI
                     PVVVTRPRRRALTVDLELLGTVAPVREVAVSARVAEEVTSTSLMLGRPVKRHEVLVEL
                     YDEMIAARLRQAEAAAAQTRSMLDKLLAGARPQEVKQAEARLEGAQAGFASAEKEYQR
                     MGSLKQNNAIPEQKAEKVIAAFEGAKAELQAAKQQLSLVREGARKEDIRMAEAAHQQA
                     LANLELARIQMRQTKMSAPIDGVISKINKELGEQTEVGKPVFSLMDLDTLHLLVDVPR
                     TRIHQVRTGQTARIVLEEPRQETVGTITEVKPDADPMSRTYLAKIQFRNPNGVFKPGM
                     FVRAFVKLDEKPDALVLPKDCLAQVATQTGLFLVNDQHRVSFAPVTVGLSSSDDIEVI
                     SDLSEKATVVLQGQKHLVQDTLVDPTPQS"
     gene            complement(3432..>4507)
                     /locus_tag="PKO06_05985"
     CDS             complement(3432..>4507)
                     /locus_tag="PKO06_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69229.1"
                     /translation="LPGFGDVRIGQNDSNISKAVLQQPLYTFGRIESGVKMIEEQVKA
                     EECALSAKKEDIVLAVVKSYLEVLKAQNRAGIAHEGHRVISEHLQLTETLFKAGVVLS
                     TDVAATRVKQLEAEQKLVEEENAVHIAEEELSHLLGLPTGELGTIADIPLGSLSLPPA
                     STTTLALRHPSSEIQRVDALLRLLRNRHFIEKRGQYPIVSLQATYETGNQFTEHQKNW
                     NAVVVLDFPIFDSGATKARASQVHHEIARTTHQRSLVEQGVELAVKTSFLRVQELEKK
                     IGLAMQALQTAQENLDQNRINYREGAALNTDVLNAELLLRNAKVAYSNAFFDYLYQQT
                     ALHKHLGRLSEYLRTVLGVDATF"
BASE COUNT          861 a         1222 c         1395 g         1029 t
ORIGIN      
        1 cttccggctt tccggtttgc aggctgctga gaacgtcgag cgtgcccggg gttcgtttga
       61 cgattgccag catctggctc tcaatgcgct gcagaacttc gagatcgtcg ccgagaatgt
      121 cgatcatgat cggcgattcg ctggtgccga gtaaccctgt ttttcggacg ttgagctcca
      181 cctcaggaat ctgcgacaat tggggcgtga tggcggcaat gaactggtcg gtgctgatcg
      241 tgaccgtgtc gtgcaggcga acgctgattt cgccaagatg cgtaccggtg gtcgaaccgc
      301 caaccagcga ctccgtcttc cccagcttgg tgaacgaaaa ccgaatttcg ggcctgccgc
      361 gtaacacatc ttcgatcttt ttcaaggcgc gatcggtatt gtcgatcgaa gaaccaggcg
      421 gaagcttgac cgtgatgtcg aattccttct ggtcaggctc agtcacgaac tcgaacccga
      481 tgaagggagc cagcagaaaa ctggcgccga acaagctgcc gcagacaagg aaggtcagca
      541 ggcggtgctt catgatcagg gtcagcagcg atcggtaatc atccgcgagt ctggtgaaag
      601 cgcggtccca ccagcgtccg aatcgatact tcttgtcggg gtcggcatcg tcggctgtca
      661 gaaagaaccc ggcgcacatg ggggtcagcg taaaggccac cagcagagac acgaaggtcg
      721 cgaaggtgac ggtcaggccg aactcgtaga aaaagcgacc gaccatgcct tccatgaagg
      781 cgatgggagt gaacacgagg acattggtca gggtgcagcc ggcgaccgcg accagaattt
      841 catttgtacc ggtgatagct gcctctttcg ggctttgttt gctcttctgc aaatggacgt
      901 aaatgttttc gagtacgatg atggcgttgt tgaccagaat gccgacgctg attgccagcg
      961 ccatcaacga catcatgttc agcgagaagc cgaaggcgta gacgagggtg taggtggcca
     1021 cgatcgagat cggaatcgag aggaccgaga cgagtgtcgc cttgaacgag tgaagaaaga
     1081 gaaacaggat gatggcggtg atgatgatac ccgcgatcag gttgccgttc aggtcgtcga
     1141 cggaatcgac gatgaaggtg gctcggttgc gcaccggcgt gaattcgata tcccgcggca
     1201 gtttgctctt gagttcctcg agacgtttca gaacgccctc ggccgtgcgc acggagttcg
     1261 catctgaccg tttcttgacg atcatggcga tgcaggggcg gtcgttcagg cgcacggctt
     1321 gccgaaactc ttcgtattcg tcggtgacct gggcgatgtc gccgagcgta tacgtcttac
     1381 ccccggcggt cggaatcggt agttcgcgaa tctcacgcac ggaggtgaac tcgccatcca
     1441 tgcggatgtt atactctttg cgagtctggg tgatgtgccc gctgggaatg ttgaggctct
     1501 tctgcgcaat catgccggcg agcgagatcg gggagatctg aaaggctttc gcgattccct
     1561 ggtcgatctg aatcgtgatc tcgcgctcgc gagcgccgat gaagtcgaga tcggcaaccc
     1621 cgtcgacacg tctgatctcg tcgcgaatga gatctttcgc cagcgcgtag gtcgactcga
     1681 ccggtcggtt gccgctgatg gccagactca gaatcggcag cgcgttgatg tcgagcttga
     1741 gaatcaccgg ctctttcgcg tcatcgggca gtttgtcgcg aatggctccg actttttcgc
     1801 gcacgtccgc ggcggcggtg tcgacgtcga tgtaatcttc gaatctgatc aagacgttcg
     1861 agaagccttc gtacgagtcg gatttcaact cgtcaatacc attgatcgcg ctgacggcat
     1921 cttcgatctt ctgactgatc agggtttcga tgttcttcgg tgacgcgccc ggatacgccg
     1981 tgacgacggt taccatcgga aaatcgattt tcggcagcat gtcgatcgga atcttgccgt
     2041 aggaaaaaag gccgagaacg atgagaaaca gcaacaccat ggtcatcatg accgggcgtt
     2101 tgatggaaag ttcgacgata ttcatgggtg tggcttcttt gtcggggaag gtcaggactg
     2161 cggcgtcgga tcgaccagtg tgtcttgtac cagatgcttc tgtccttgaa gcacgacagt
     2221 cgccttttca gaaagatcgg aaatgacttc aatgtcgtct gagctgctca ggccgacggt
     2281 gacgggggcg aagctgaccc ggtgctgatc gttgaccaga aacagacctg tctgggtggc
     2341 gacctgagcc agacagtcct tcggaagcac cagcgcgtcg ggcttttcgt ccagttttac
     2401 aaaggcgcga acgaacatcc cgggcttgaa gactccattc ggatttctga attgaatctt
     2461 tgccagatac gtgcgtgaca tcgggtcggc gtctggcttg acctcggtga tcgtgccgac
     2521 cgtttcctgc cgtggttctt caagcacgat ccgggccgtc tggccggtac gaacctggtg
     2581 aatgcgggtg cgtggaacat ccaccaatag atggagagtg tccaggtcca tcagtgagaa
     2641 gaccggcttt ccgacttcgg tctgttcacc caactccttg ttgattttcg agatgacgcc
     2701 atcgatgggc gcagacattt tcgtctgtcg catctgaatg cgcgcgagtt caagattcgc
     2761 cagagcctgt tggtgagcgg cttcagccat gcgaatatct tccttgcgcg cgccttcgcg
     2821 aaccaggctc aactgctgtt tggccgcctg cagttcggcc ttcgcgccct cgaaggcggc
     2881 gatgactttc tcggccttct gttccggaat cgcgttgttc tgtttcagcg atcccatgcg
     2941 ctggtactct ttctcggcgc tggcgaatcc ggcttgggcc ccttcgaggc gcgcttcggc
     3001 ctgtttgact tcctgcggcc gggccccggc gagcagtttg tcgagcatcg agcgggtctg
     3061 cgccgccgcg gcttcggcct gccgcaggcg cgcggcgatc atctcatcgt acagctcgac
     3121 gaggacctcg tggcgtttga ccgggcgacc gagcatgaga ctggtactgg tgacttcttc
     3181 ggccacgcga gccgataccg cgacttcacg aaccggcgcg acggtgccga gcagctcgag
     3241 atcgacggtc agggcgcggc gtcgagggcg ggtgacgacg accggaatgc ctttgcgggt
     3301 ctgatagtca gagacgttca gggctttggg ggtgttgcga atggctgaca atcgcgccgt
     3361 gactccgaag ccgatggcgg cgaccaggac gatgccgaga atgagatact ttttcatgtg
     3421 cgtgctccga ttcagaaggt ggcgtcgacg ccgaggaccg ttcgcagata ctctgacagt
     3481 cgtcccaggt gcttgtgcag ggcggtttgt tgatacaagt agtcgaaaaa agcgttgctg
     3541 taggccactt tggcgtttcg cagcaggagt tccgcgttga gaacgtccgt gttcagagcg
     3601 gctccctcgc gatagttgat gcgattttga tcgaggttct cctgcgcggt ctgcaacgcc
     3661 tgcatggcga ggccgatttt cttctccagt tcctgcactc gcagaaaact ggttttcacc
     3721 gccagttcga ccccctgttc caccaacgac cgctgatggg tcgttcgggc aatctcgtga
     3781 tggacctggc tggcacgagc tttggtggcg ccgctgtcga agattggaaa atccagcacg
     3841 acgacggcgt tccagttctt ctggtgctcg gtgaactgat tgccggtttc gtaggtcgcc
     3901 tgcaggctga cgatcggata ctggccgcgt ttttcaatga agtgccggtt gcgcagcagg
     3961 cgcagcagcg cgtcgacgcg ttgaatctcg gatgacgggt gccgcagagc cagcgtggtg
     4021 gtcgaggcgg gcggcaggct gagtgacccg agtggaatgt cggcgatcgt tcccagttca
     4081 ccagttggca gaccgagcag gtgactcagt tcctcttcgg cgatgtgaac tgcattctct
     4141 tcctccacca gcttctgctc ggcttcgagt tgtttcacgc gggtggcggc cacatccgtg
     4201 ctgagcacca cgccggcttt gaacagggtt tcggtcagtt gcagatgctc gctgatcacc
     4261 cgatgccctt cgtgggcgat accggcacgg ttctgtgcct ttagaacctc gaggtaactc
     4321 ttgacgacgg ccaggacaat atcttccttt ttggcgctca gagcgcattc ttcagccttg
     4381 acctgttctt cgatcatctt caccccggac tcgatgcgcc cgaacgtgta gagcggttgt
     4441 tgcagaacgg ccttggaaat gtttgagtcg ttctgaccga tgcggacatc gccgaaacca
     4501 ggcagcg
//
LOCUS       DAORHD010000220         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20533_length_4494_cov_4.108358, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000220 DAORHD010000000
VERSION     DAORHD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20533_length_4494_cov_4.108358"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2536
                     /locus_tag="PKO06_05990"
     CDS             <1..2536
                     /locus_tag="PKO06_05990"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69230.1"
                     /translation="AEDGDSGRRRYVPFILIALFYVIAHSLPTIFAFLESLYWPWIRT
                     LMRFAPSVALGSLEGRIGRIRDAEQKRLQYELLTQAAFRARVPEKGVRYAQLTLEIDH
                     ENLVAVDALGSYFLGRAEGLVQQLDIIIKYVNRHLQEKGLLEKFVQQMLQRREFKPEM
                     LPIVKKYLSYFPQNTDLINFAAEVLRSWRATDVGREGMELLEKSFQISQDADLGQILW
                     RVYVACGMRDQAVTLATSLASAGHAVDMIGLLDRLEEDIDQKVLFLQQAIMGMEKARQ
                     LEAMQAVHDLHHITTMQAARLIQPLEGFEGEADVNIKFHAQKARDHLKRISLECKTFR
                     DRLRSLQSTPTPISTPEFVPDVSTAETVPTEEPLINFPGLPEQPQDAPLDVVGDSVPG
                     DHSSPEAGQVPETSAADDSFDPFRITAPRSESSEEPGVPGDLPLPALESAPTELESPV
                     ADEPNLSPRSVEPDNPPDTPAADVPLEEGLRLPSLNDLASSESQAEDALTPVPPESAP
                     APAPASVMEQRLFAELQSFDFAEDVTRMPATEQSRLFDDLPTRFAGETSVVENPPTDA
                     SPAVKEHLSNSMTQADVPAVAGESATTVHSESGKTPESDSPVSTGVDTVEPSFDASLT
                     DLILGLGENPSEEQLNNLRVLTTVDDLTAWDLYLKQPHEDVTICAVLTMFGGMNLSGV
                     PARLLPFLRHPSSVVRERAVEVLTALGSLDVLPDLALRSSDPLPEARECVARVLAGYD
                     PERILPELKNWLSNADTDKRDLAIRLLKGIRVNESSGMLQTLLYDSEAIIRRNAIRAL
                     AVQALPGNARILHEYLDVCTDAEEETLIAKAIDFLENQRSDSPFQE"
     gene            complement(2558..3658)
                     /locus_tag="PKO06_05995"
     CDS             complement(2558..3658)
                     /locus_tag="PKO06_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV69231.1"
                     /translation="MATTSTSKKGGIQGEFGINDLLQLVVEKDGSDLHLTVGAPPMMR
                     LSGKLFATDLPILTHKDTQRLTFEFLNNEQRERFERDLELDISYELENVSRFRVNLFW
                     NRRGIGGVFRVIPTRIKSLTELKMPSVLADLARRNKGLVLVTGPTGSGKSTTLAAMVD
                     MINSERQEHILTIEDPIEFVHNHKRCIVNQREIGPNTKSFSNALRAALREDPNVILVG
                     EMRDYETISLAVTAAETGHLVFATLHTPSAAQSVDRIVDVFPSHQQDQIRTQLADALQ
                     GIVAQQLIPTADGMGRCVALEIMINIPATSNLIRENKTFQITSIIQTHKQTGMQTMDQ
                     ALLDLVKSRKITPEEAYRRAFDKKAFEMYKPK"
     gene            complement(4009..>4494)
                     /locus_tag="PKO06_06000"
     CDS             complement(4009..>4494)
                     /locus_tag="PKO06_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69232.1"
                     /translation="TRVLDGCAGLGGKTIITSFPAYDAASSVVKLLEQSPDPVTAAMS
                     LAGFVSQRLIRQLCATCKKSIETPPELAERLKDSLVTPTCAQAVGCDACQNTGFSGRL
                     LVHEFIPVSSVLRQMMINRQSYQDFCQLARKDGILSLEQQVLDLVVRGETSYDEFLRF
                     F"
BASE COUNT          899 a         1196 c         1401 g          998 t
ORIGIN      
        1 tgcggaggac ggcgacagcg gtcgtcgtcg gtacgtcccg ttcattttga tcgctctctt
       61 ttatgtcatc gcgcactctc tgccgacgat ctttgccttt ctcgaaagcc tgtactggcc
      121 atggatcaga accctgatgc gtttcgcccc atcggttgct ctcggctcac tcgaaggacg
      181 tattggtcgg attcgggacg ccgaacagaa acgtctgcag tatgaactgc tgactcaggc
      241 tgcgtttcgg gcgcgagtac cggagaaggg cgttcgctat gcgcaactga cgctggaaat
      301 cgaccatgag aatctcgttg ccgttgatgc gctgggctct tactttctcg ggcgagccga
      361 aggattagtg cagcagctcg atatcatcat caaatatgtg aatcgccatc tgcaggaaaa
      421 gggattgctc gaaaagttcg tgcagcagat gctgcagcgt cgcgagttca agccggaaat
      481 gctgccgatc gtgaaaaagt acctgtcgta ttttccacag aatacagatc tgatcaactt
      541 tgccgccgaa gtgctccgca gttggagagc gacggatgtc ggtcgggaag gcatggaact
      601 cctggaaaaa tctttccaga tttctcagga tgccgacctg ggacagattc tctggcgcgt
      661 ctatgtggcc tgtggcatgc gtgatcaggc cgtgactctg gcgacctcgc tggcctcggc
      721 cggacacgcg gttgacatga tcggcctgtt ggatcgattg gaagaggaca ttgatcagaa
      781 ggtgctgttt ctgcaacagg caatcatggg tatggaaaag gcccgacagc tcgaagcgat
      841 gcaggcggtt cacgacctgc accatatcac gacgatgcag gcggcgcgtc tgattcagcc
      901 tctcgagggg ttcgagggcg aagcggatgt caacatcaag tttcatgcgc agaaagcgcg
      961 tgatcatttg aagcgcatca gtctcgagtg caaaaccttc cgcgatcgat tgcgatcgtt
     1021 gcagagcact ccgacaccaa tctcgacgcc cgaatttgta ccagatgttt cgacggcgga
     1081 gacagtgccg accgaagaac cgctgatcaa ctttccgggt ttgcccgaac agccgcagga
     1141 tgcgcctctt gacgtcgtcg gtgactccgt gccaggagat cactcgtcgc ctgaggccgg
     1201 gcaggttcca gaaacgtcgg cggctgacga ttcgtttgat ccgtttcgaa tcaccgcacc
     1261 ccggtcagaa tcttctgagg aaccgggcgt tccgggcgac ctcccccttc cggcgttgga
     1321 gtccgcccct acggaacttg agtcgccggt cgccgatgag ccgaatcttt ctccgcgttc
     1381 ggtcgagcct gacaacccgc cagacacgcc tgcggctgat gtgccgctgg aagaggggtt
     1441 gcggttgcct tccctgaacg atctcgcgtc ttcggagtct caggcagagg atgctctgac
     1501 tcctgttccg ccagaatcgg ctcccgctcc ggctcctgcc agtgtgatgg aacagcgctt
     1561 gtttgccgag ctgcagtcgt ttgattttgc tgaggacgtc acacgcatgc ctgccacgga
     1621 gcaatcgaga ttgtttgatg atttgccgac gcgctttgcg ggagagactt ccgttgttga
     1681 gaacccaccg accgatgcgt ctccggccgt gaaagagcac ttgtcgaact cgatgacgca
     1741 ggccgatgtg ccggctgtcg caggcgaatc cgcgacgacg gtacattctg aatcgggtaa
     1801 gacacccgaa tcagattcgc cggtatcgac cggtgtcgat actgtagaac cctcttttga
     1861 cgcgagtttg accgatctga tactcggact cggagaaaac ccttctgagg aacagctgaa
     1921 caatctgcga gtgttgacca ccgttgatga tctgaccgca tgggatctct atctgaagca
     1981 gccgcacgaa gatgtgacca tctgcgctgt tctcacgatg tttggcggca tgaatctgtc
     2041 cggagttccg gcgagactat tgccctttct gcggcaccct tcatccgtcg tgcgtgaacg
     2101 agcggtcgag gtgttgacgg ccctgggctc gctcgacgtt ctgccggacc ttgctttgag
     2161 atcgtctgat ccactccctg aagctcgtga gtgtgtcgcg cgggtgctgg ccggctatga
     2221 tcccgaacga attcttcccg aactgaaaaa ctggttgagt aacgcagaca ccgataaacg
     2281 cgacttggcg attcgtctgt taaaggggat tcgcgtgaac gagtcttcgg ggatgctgca
     2341 gacgttgctc tacgactccg aagcgatcat tcggcgcaat gccattcggg cgcttgccgt
     2401 gcaggcgtta cctggcaatg cccgcattct gcacgaatat ctggatgtct gcacggacgc
     2461 ggaagaagag acactgattg ccaaagcgat cgactttctc gagaaccagc gctccgatag
     2521 cccattccag gaatagactc gtgtgggcgg cgagcgtcta cttcggcttg tacatctcaa
     2581 acgccttttt gtcgaaggcg cgccggtagg cttcttccgg agtgatcttt cgtgacttga
     2641 ccaggtcgag gagagcctga tccatggtct gcataccggt ttgcttgtgc gtctgaatga
     2701 tcgaggtgat ctgaaaggtt ttgttttcgc gaatcaggtt ggaagttgcg ggaatgttga
     2761 tcatgatttc gagagcgacg cagcgtccca ttccgtcagc ggtgggaatc agttgctgag
     2821 cgacaatccc ctgcagggca tccgcgagct gagtgcgaat ctggtcctgc tggtgcgaag
     2881 ggaaaacgtc gacgatacga tcgacggatt gggcggctga tggcgtgtgc aacgtcgcga
     2941 acaccaggtg acctgtttcg gcggcggtaa cggcgaggga gatggtttcg taatcgcgca
     3001 tttcgccgac gagaatgacg ttgggatctt cgcgcagagc cgcgcgcaga gcattggaaa
     3061 acgacttggt attcggcccg atttcgcgct gattgacgat acagcgcttg tgattgtgca
     3121 caaattcgat cgggtcttcg atcgtgagaa tatgttcctg gcgttcgctg ttgatcatgt
     3181 cgaccatcgc ggcgagcgtg gttgattttc ctgaacccgt gggcccggtg acgagtacca
     3241 gtcctttatt gcgacgggcc agatcagcaa gtaccgaggg catcttcagt tcggtcagag
     3301 atttgatacg ggtgggaatg acgcggaaga cgccgccgat accgcgacgg ttccagaaga
     3361 ggttgacgcg gaagcgcgag acgttctcga gttcatacga aatgtccagt tcgagatcgc
     3421 gttcaaagcg ctcgcgttgc tcgttgttga gaaactcaaa cgtcaaccgc tgggtgtctt
     3481 tgtgcgtgag aatcggcaga tcggtagcga agagtttgcc tgaaagacgc atcatgggcg
     3541 gcgcgccaac ggtcaggtga agatcggaac cgtccttctc gacgacgagt tgcagcaggt
     3601 cgttgatgcc gaactcgccc tggatgccac ccttcttcga ggtggacgtc gttgccatca
     3661 ttgaagtcgg tgggcggaag ctggggccag cggcaggtgc agccgggctt gccggagcaa
     3721 ccggcgcggc gggcgcggga ccgggtggcc gtggtgtcgc ccctggagcc cctgcggcag
     3781 gggatggggg gcgaaaggtt ggtgccgcct gaccgggagc gcccgccgga gctccggggg
     3841 ggcgcggtgg ttggggcggt tgcgcggtat tggctgtggg cggccgtggt ggggctgccg
     3901 gcggagtacc ggtcgggttg ctcgcaggag cgttcccgtc gggcttttcg ccgccaaata
     3961 atctgtcgaa gaaggtgtct ttgtcagcca tgtgttgtca agattggttc agaagaaccg
     4021 gagaaattcg tcataactgg tttcaccgcg aaccaccaga tcaagaacct gctgttcgag
     4081 gctgaggatg ccgtctttgc gcgccaattg gcagaagtcc tggtagctct ggcggttgat
     4141 catcatctgg cgcagaacgg aactgaccgg aatgaactca tgaaccagca atcggcctga
     4201 aaatccggtg ttctgacagg catcgcagcc gactgcctga gcgcaggtgg gagtcaccag
     4261 cgaatctttc aatcgttcag ccagttccgg gggagtctcg atagatttct tacatgtggc
     4321 gcagagttgg cgaatcagtc gctgtgagac gaacccggcc aacgacatgg ccgcagtcac
     4381 cgggtcggga gactgctcga ggagcttcac gacactgctt gcagcgtcat atgccggaaa
     4441 cgaggtgatg atcgtcttgc cgcccaaacc ggcgcagccg tcaagaacac gggt
//
LOCUS       DAORHD010000221         4466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20731_length_4466_cov_4.172297, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000221 DAORHD010000000
VERSION     DAORHD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20731_length_4466_cov_4.172297"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="PKO06_06005"
     CDS             <1..999
                     /locus_tag="PKO06_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV69233.1"
                     /translation="ETHLDPRHEIRLRYEVTPDQRGDFLFGDITVRYRGVLELVEIQQ
                     TFPARVKVEVYPNIRNISRLDLSLKRSHLVESGLITERRRGSGTEFESLRAYVRGDEF
                     RKIDWKASARKSILITRNFQNETNQSVMLLIDQSRTLGVKVGRHTLLDAAVNAALLIG
                     HYIVRKEDKVGLLTFARQIHAYTPPRRGKRHFHELLRSLYNLQPRTDEADFMPAFRHL
                     ATARLRRSLILLFTDLSSGNGLDSLLKSISLLAGRHVLVVVSLIDPQIRKVAESTPTT
                     YEEVARKAVALDLLSRFSQIRRELEQKGLGLLVLTPEEANSAILATYIRLKLREIL"
     gene            complement(949..2076)
                     /locus_tag="PKO06_06010"
     CDS             complement(949..2076)
                     /locus_tag="PKO06_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HNV69234.1"
                     /translation="MTETTEERRLKAALQPRYKASEAERARWQKLHQLIVQIEARRGL
                     AGLIRVGIETIMGGKQRARWLFGDAPDLAYSLADLSSLYQSAVSDLAKARSQKADPVL
                     IEQLNLLVGRAYAVIYGRPGVQIMDVVRFFLADFPRLVRERRHFLSVAVLVFLCGYLV
                     GFLCFDMDSKLIGLVIPENMRQALQQAIAQGKVGGGINTLQRFNEASRIMFHNMEVGF
                     LAFATGLGFGIGTVYLLILNGMMIGGLAAIYHQAGLAKEFWALILPHGAVELPCIFIA
                     GAGGLMLGYALLNPGRYRRSEWLNHEGYDAVRLVVGTLPLLVAAGLVETYLTPSTLPI
                     PAKYAAAMILGACAAAYLFLPPGWVLQYFSQLQPDIRRQNR"
     gene            complement(2073..2588)
                     /locus_tag="PKO06_06015"
     CDS             complement(2073..2588)
                     /locus_tag="PKO06_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69235.1"
                     /translation="MEERLCRICGHVGEYRQRFCRGCGSELIDEDLPLEQTLVLDFPE
                     QGVVEQSTSGCARVFYALVGIFILFPLLFEVVEVFGGGRQRRSEDARRRACYANMRVI
                     LGAVEMYNMDHHMMRDSVADSDVCSPEGLLVRESYLKSPITKPELDCSYSGYGLTKDG
                     KIKCQRHGSVE"
     gene            complement(2652..3800)
                     /locus_tag="PKO06_06020"
     CDS             complement(2652..3800)
                     /locus_tag="PKO06_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV69236.1"
                     /translation="MAFWNQLQSVIQWKDPAPNSLIWRWNGGTDELKNASKLIVGPGQ
                     AAIFVYQGRVEAIHLEQGTFEIRTANIPFFTTLSKFMQGFESEHKAEVYFVRMTEITN
                     QKWGTKSPVKYEDPKYKFPVGMRAFGNFSFKITKPAAFFQDIAGARQEYTIDEIRTAI
                     VDRIITPLADLFAESGFSYAEIDKQRVELSKRLREMLAAEFSQLGFELTDFRIENTDF
                     DDDTKGRISRISDKIADAHAIKAMGDIDSTSMRNYAAVEQLNALKSAANNPSGTAGLG
                     VGMGAGIGLGQNMAQMMNQPPAGAAATATCGHCQAGIPAGVKFCPQCGKPAVAAGQAA
                     CVACGKPMAAGAKFCPECGAAQKAACPSCKAEMAPGAKFCSNCGHKTN"
     gene            complement(3878..>4466)
                     /locus_tag="PKO06_06025"
     CDS             complement(3878..>4466)
                     /locus_tag="PKO06_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV69237.1"
                     /translation="CLVALYAGHSIWARESRLISVEVALEHSQGKKCPRCWGEMSADA
                     QFCGLCGKPLPGSEPEAPAWYAFTEQTEKIWYVVLICCVVYAGAITETPCPHCGDKHS
                     VLFGNWRGPRCSARVKHCYSNMRVLLGAIEMYNMDNAVMLDSLQDADGSAGGLLLQRQ
                     YLKSPITKPTVKCAYSGTNLTKNGRISCAFHGTVE"
BASE COUNT          973 a         1385 c         1201 g          907 t
ORIGIN      
        1 gaaacccatc ttgaccctcg tcacgaaatc cgtctgagat acgaggtcac tcccgatcag
       61 cgcggagact tcctgttcgg cgacatcacc gtccggtacc gcggcgtcct cgagcttgtc
      121 gagatccagc agacctttcc ggcacgcgtc aaggtcgagg tgtatccgaa cattcgcaac
      181 atctcgcgcc tggatctgag tctgaaacgc agccatctcg tggagtcggg tctgatcacg
      241 gaacgccgcc ggggctctgg cactgagttc gagagccttc gcgcgtatgt ccgaggcgac
      301 gagtttcgca agattgattg gaaggcttcc gcgcgcaaaa gcatcctgat cacccgaaac
      361 ttccagaatg agacgaacca gtctgtgatg ctgttgatcg atcagagccg caccctcggg
      421 gtcaaagtcg gacgtcacac cctgctcgat gcggcggtga atgccgccct gctgatcgga
      481 cactacatcg tccgcaagga agacaaggtc ggcctgctga cctttgcgcg ccagatccac
      541 gcctataccc ctccacggcg aggcaaacgc cactttcacg aactcctgcg cagtttgtac
      601 aatctgcagc cccgcaccga cgaagccgac ttcatgccag cctttcggca cctggccacc
      661 gcacgcttgc gtcggtctct cattctgctg ttcaccgatc tttcgagcgg aaacggcctg
      721 gacagtctgt taaaatcgat ctcgctgctg gcgggtcgtc atgtactggt ggtcgtcagc
      781 ctgattgatc ctcaaattcg taaggtcgcc gagtcgactc ctacaaccta cgaagaagtc
      841 gcgcgcaaag cggtcgcact cgatcttctg agtcggttca gccagatcag acgtgaactc
      901 gagcagaagg gactcggact tctggttctg acaccggaag aagcgaactc agcgattctg
      961 gcgacgtata tccggctgaa gctgcgagaa atactgtagc acccaccccg gcggcagaaa
     1021 cagataggcg gcggcacagg cgccgagaat catggctgcc gcatacttcg ccggaatcgg
     1081 cagagtcgac ggtgtcagat aggtctcgac cagaccggcg gccacaagaa gaggcagcgt
     1141 gccgacaacc agacgtacgg cgtcgtatcc ttcatggttg agccattcgc tgcggcgata
     1201 gcgtccgggg ttcaagagcg catatcccag catcagaccg ccggcaccgg cgatgaaaat
     1261 gcagggcaac tccacggctc cgtgcgggag aatcaaagcc cagaactctt tggccaaacc
     1321 cgcctgatgg taaattgccg ccaggccacc aatcatcatg ccattcagaa tcagcaggta
     1381 gacagtacca ataccgaaac cgagaccggt cgcaaacgcg agaaaaccca cttccatgtt
     1441 atggaacatg atgcgactcg cttcattgaa gcgttgaagg gtattgatcc cgcctccgac
     1501 cttgccctga gcgatcgcct gctgcagggc ctggcgcatg ttttcgggaa tcacgagtcc
     1561 gatgagtttc gaatccatgt caaagcagag aaatccgacc agatacccgc acaggaacac
     1621 cagcaccgcg acgctcagaa aatggcgacg ctcccggacc agacgcggga agtcggcaag
     1681 gaaaaaccgc acgacatcca tgatctgaac gcctgggcgc ccgtagatga ccgcataggc
     1741 tcgccccacc agaagattga gttgttcgat gagcaccggg tctgctttct gcgacctggc
     1801 cttcgccagg tcggaaaccg cgctctgata cagtgaactc aggtctgcca gtgagtaggc
     1861 gagatccggc gcatccccaa acagccagcg ggcacgctgc ttccctccca tgatggtctc
     1921 gatgcccacg cggatcagac ctgccaaccc acgacgggct tcgatctgca cgatcaactg
     1981 gtgcaacttc tgccagcggg cccgctcggc ttcagaggcc ttgtatcggg gctgcagcgc
     2041 ggccttcaat cgtcgttctt cagtggtttc cgtcattcga ccgatccatg tctctggcac
     2101 ttgatctttc cgtctttggt cagaccgtag ccggaatacg agcaatccag ctctggcttg
     2161 gtaatcggcg acttgagata gctttcccgg accagcaatc cctccggcga gcacacatcg
     2221 ctatccgcca cggaatcgcg catcatgtgg tgatccatat tgtacatttc gacggctccg
     2281 agaatcaccc gcatgttggc ataacaggca cgtcgccggg catcttcgga tctccgttgt
     2341 cgaccgccgc caaagacctc gacgacttca aacagcaagg gaaacaggat gaaaatgcca
     2401 acgagcgcat agaacacacg agcgcagccg gaggtggact gttccaccac tccctgctcc
     2461 gggaaatcga gaaccagcgt ctgttcgaga ggcaggtctt catcaatcag ctcgctgcca
     2521 catccacggc agaatcgctg acggtattct ccgacatggc cacagatccg acaaaggcgt
     2581 tcttccatac actcagcaaa aacccgcccc aattggggcg ggcaggaaat ctctcatgaa
     2641 cctgtaccag ttcagttcgt tttatgtccg cagttggagc agaatttcgc ccccggcgcc
     2701 atctccgcct tacaggaggg acacgccgct ttctgagccg ccccacattc ggggcagaat
     2761 ttcgcgccgg cggccatcgg cttcccgcac gccacgcacg cggcctgacc agcggcaacg
     2821 gcaggcttgc cacactgcgg gcagaacttg accccggcag gaatgccggc ctgacaatgg
     2881 ccgcaggtcg ccgtggctgc cgccccggcg ggtggctggt tcatcatctg ggccatattc
     2941 tgacctagtc cgatcccggc tcccatgccg actccaagac cagctgtccc ggacggattg
     3001 ttggcggcac tcttcagtgc gttcaactgt tcgaccgcgg cataattgcg catactggtg
     3061 ctgtcgatat ctcccatcgc cttgatcgcg tgcgcatcgg cgattttgtc cgagatgcga
     3121 ctgatgcgac ccttggtgtc atcgtcgaaa tcggtgttct caatacgaaa atccgtcagt
     3181 tcgaacccca gttgcgaaaa ctcggccgcc agcatctcac gcagacgctt cgacagttcg
     3241 actcgctgtt tgtcgatctc cgcgtaactg aaaccggatt cggcaaacag gtccgccagg
     3301 ggagtgatga tgcgatcgac gatcgcggtg cgaatctcgt caatcgtgta ttcctgccgc
     3361 gcgcccgcga tgtcctgaaa gaaggccgcc ggtttcgtga tcttgaaact gaagttcccg
     3421 aaggccctca tgcctaccgg gaacttgtac ttcggatctt catacttgac cggagacttg
     3481 gtgccccatt tctggttggt gatctcggtc atgcggacga agtagacttc cgccttgtgc
     3541 tccgattcga acccctgcat gaatttgctg agggtcgtaa aaaacggaat gttggcggta
     3601 cgaatctcaa aggttccctg ttcgaggtga atggcttcca ctctgccctg atagacaaag
     3661 atggccgcct gaccaggccc cacgatcaat ttggaggcat tcttcaattc atccgtgccg
     3721 ccgttccagc gccagatcag cgaattcggg gcgggatcct tccactggat caccgactgc
     3781 aactgattcc agaaagccat ggagtcctcc gtttttcgag aaaattacac cgccgagcgg
     3841 ttctcgtctt caggttacca tatccctggc cgggggtcta ttccaccgtc ccgtgaaatg
     3901 cacaactgat ccggccattt ttcgtcaaat tcgtgccgga ataagcgcac ttcaccgtcg
     3961 gcttggtgat cgggctcttg agatattgac gttgcagcag cagccctccc gcggatccgt
     4021 ccgcatcctg cagcgaatcg agcatgaccg cattgtccat gttgtacatt tcgatggctc
     4081 cgagtaacac gcgcatattg gagtaacagt gcttgacccg ggccgagcac cgtgggcccc
     4141 gccagttacc gaacaaaacc gagtgtttgt cgccacagtg cgggcacgga gtctccgtga
     4201 tagctcccgc gtagaccacg caacagatca ggacgacgta ccagattttt tccgtctgtt
     4261 ccgtgaacgc ataccaggca ggcgcttcgg gctctgaccc cggaagcggc ttgccgcaga
     4321 ggccgcagaa ctgagcatca gcgctcatct caccccaaca acgaggacac tttttccctt
     4381 gtgaatgttc cagagcgact tccactgaaa tcaacctgga ctctcgcgcc cagatcgagt
     4441 gtccggcata cagagcgacc agacaa
//
LOCUS       DAORHD010000222         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20800_length_4457_cov_3.844616, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000222 DAORHD010000000
VERSION     DAORHD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20800_length_4457_cov_3.844616"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PKO06_06030"
     CDS             <1..1150
                     /locus_tag="PKO06_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNV69238.1"
                     /translation="ASISVGHTHPLVTARVHDQIAKLNHCTMLYQTRPLLTYLQAIQA
                     ELPDHLCRHFFVNSGSEAMDFACQAARAHRQKPLLIAFSEGFHGGSFQSASVTGLPAW
                     QPVYGADPQVAYVPVRSCRFCPHYSSSGLRTKRLQPLLHQCPAVCLDPLEDLLRQRAP
                     DAAAILFEPVLGVGGILVPCLGFFERLQHLVDQYHLDLVCDEVQSGFGRLGYQLFGFQ
                     VFHLRPDIVCMAKGIANGYPMGLVSATEEVSAAMGGKPHFSTFGGNPVSCTAALATLE
                     TIKREQLILNAQNTGEHLLQLLDEYLLLSPFVLEIRGLGLMIGIEFDTAERAHQVVEH
                     CFERGLLVGLGGRERNVVRIEPPLTFTIDMALRTAETLAAALQATRIS"
     gene            1345..2811
                     /locus_tag="PKO06_06035"
     CDS             1345..2811
                     /locus_tag="PKO06_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNV69239.1"
                     /translation="MKTRKDITAVILDHFERLSAVPRASKKEQAIAAWLEAWATKRGL
                     PVKKDQVGNLLISVPASRGREKALPVILQGHMDMVCEKTPDSPHNFDTDPIRLVRDGD
                     WLHADRTTLGADNGIAIAMAMAVAESTDLVHPPLELLFTVDEESGMSGALGLQKGFFS
                     GRTLINLDSEDEGVFTIGCAGSRLTRLFLPLFYESVPTGYSAWRLKAKGMRGGHSGVN
                     INEERANAIRVLVRALRMAAHECDIRLILIQGGTAHNAIPRDAEALFYAPCQTGDSLA
                     ALTATIARAHEMLRAEFQATDPELALELEPFSQPADDRAMTPTCSAHVLDFLYALPHG
                     VTTRAPGQPGLVETSNNMAKAEIRNGCLEILSSQRSLVMSRLDALTQKLEAIARLSGA
                     TYQSNVGYPSWQPDFESPFIRTCAGLYKKLFKKEARVEVIHAGLECGIIGALIPGMQM
                     VSIGVTLRDPHSPRERILISSIDPVWKFLVAMLDDLAN"
     gene            complement(2863..4158)
                     /locus_tag="PKO06_06040"
     CDS             complement(2863..4158)
                     /locus_tag="PKO06_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69240.1"
                     /translation="MRHSSWIFGLLLLSLAMLTTGAAASPATMPAIPETHDEEEIGSA
                     DPLESLARYLLDKSGRVQGEAAEEPWQRLLRNEAFSAFLRSNGLADGSRLARNERYPQ
                     VAAFLRESLRPFSDPLFLETRQRESMARSLFFRRDPIREPGLFAADGYRTLHEISARN
                     RVRVSRLLPAVTEKNEVRELVQKLSVVFIHNTHVIRSVPDTPLVSSRVLQEVSGIGGL
                     NTYYFNRQFLHSDDNIFFFVLLQSGPKLGNARESLYGKYKFAPEETFGRERGWVSAFV
                     MYPYDLYRFAFGSEKTLADQLTPAFQREFPREMTTSGGDLQSLVKSLRPYKRDNPRWN
                     RMLRDSFPLWRPVRRRLGQLDFTVDDFRALTLQVIEAYLAGISTAEPARFAEWKSRLE
                     QGDTGDLMRLAYKEICGLDLYFEFKVPVAIPPWGLREIE"
     gene            4233..>4457
                     /locus_tag="PKO06_06045"
     CDS             4233..>4457
                     /locus_tag="PKO06_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69241.1"
                     /translation="MSCCSTLSGTVRQRSRSVFLLLVLAVLIPLCLQGRPSKPAFRFG
                     KAPEQPPRTAHLVVLATSDVHGELLPYDEIA"
BASE COUNT          877 a         1435 c         1255 g          890 t
ORIGIN      
        1 ggccagcatc tctgttggtc acacgcatcc cctcgtcacg gcacgcgtgc acgaccagat
       61 cgcgaagttg aaccattgca ccatgctgta tcagacccgg ccgctgctta cgtatctgca
      121 agccattcag gccgagcttc ccgaccatct gtgccggcat ttcttcgtca attccggttc
      181 cgaagccatg gacttcgcct gtcaggccgc ccgcgcgcat cgccagaaac cattgttgat
      241 cgccttcagc gaagggtttc acggcggctc cttccagtcc gcgtccgtca ccggcctgcc
      301 cgcctggcaa ccagtctatg gggctgatcc ccaggtggcg tacgtccccg ttcgctcctg
      361 ccggttttgt ccccattatt cgagcagcgg gctgcggaca aagcgactgc aacccctgct
      421 gcaccaatgt ccggccgtct gtctcgaccc gcttgaagac cttctgcgac aacgggctcc
      481 tgatgccgct gccattctat tcgaaccggt actcggcgtc ggcggcattc tggttccctg
      541 cctcggtttc ttcgaacgtc tgcagcatct ggtcgatcag taccatctcg atctcgtgtg
      601 cgacgaggtt caaagcggct tcggcagact tggttaccag ttattcggct ttcaggtctt
      661 tcatcttcgg cccgacatcg tctgcatggc caaggggatc gccaacgggt atccgatggg
      721 tctggtcagc gcgaccgaag aggtttccgc cgccatgggc ggaaaaccgc acttttccac
      781 gttcggcggg aacccggtct cgtgtaccgc cgctctggcc actctcgaaa cgatcaagcg
      841 cgaacagctg attctgaacg ctcagaatac gggtgagcat ttgttgcaac tgcttgacga
      901 gtatctgctg ctcagtccgt tcgtcctcga aattcgcggc ctgggtctca tgatcggcat
      961 cgagttcgac acggccgagc gagctcatca ggtggttgag cactgtttcg aacgcggtct
     1021 gctggtcggt ctcggtggcc gcgagcgcaa tgtcgtccgc atcgaacccc cgctgacctt
     1081 caccatcgac atggccctgc gtaccgccga aaccctggcc gcggccctcc aggcgacccg
     1141 catctcctga agaccgcgtt cctcccctcg tgaggctgcc gtcccgaccc cgcgaaccgg
     1201 cggcgacggc gaatcgttga gcgatgccaa gccgaaaacc caaacctgag aatcctatga
     1261 ccctggaccc tcgcccggcg gggttttctt cgctgcggcg gaatggtatg atgcggccat
     1321 cgtcactgca gggggaggat acccatgaag acccggaaag acatcaccgc cgtcattctc
     1381 gatcacttcg agcgcttgag cgccgttcca cgcgcctcga agaaagaaca ggccatcgca
     1441 gcctggcttg aggcttgggc gaccaaacgt ggtctgccgg tcaagaaaga tcaggtaggg
     1501 aatctgctca tttcggtgcc agcgagtcgc ggtcgtgaga aagcgcttcc ggtgattctg
     1561 caggggcata tggacatggt ctgcgagaaa acccccgact ccccgcacaa ctttgatacc
     1621 gacccgatcc gtctggtccg tgacggtgac tggctgcacg ccgatcgcac gaccctggga
     1681 gcagacaatg ggatcgccat cgccatggcc atggccgtcg ccgagtcgac cgatctggtc
     1741 cacccgcccc tcgagttgtt gttcaccgtc gatgaagaga gtggaatgtc cggggcactc
     1801 ggtctgcaga agggcttctt cagcggtcgc acgctgatca atctcgattc cgaagacgaa
     1861 ggggttttca ccatcgggtg cgccggcagc cggctgaccc gcttgtttct accgttgttc
     1921 tacgagtcgg ttcccaccgg ttactccgcc tggcggttga aagcgaaggg gatgcgcggc
     1981 gggcactcgg gcgtcaacat caacgaggaa cgggcgaacg cgattcgcgt cctggtgcgc
     2041 gcactccgca tggcggcaca tgaatgcgac attcgcctca ttctgattca gggcggcacc
     2101 gcccacaacg cgattcctcg cgacgccgag gcactgttct acgcaccctg ccagacaggc
     2161 gactctctcg cagccttgac cgccactatc gcccgcgcac acgaaatgct gcgggccgag
     2221 tttcaggcca ccgaccccga actcgcgctc gaactcgaac ccttcagcca acccgcggac
     2281 gatcgggcca tgaccccgac ctgctccgcc catgtgctcg actttctcta tgccctgccc
     2341 catggtgtca cgacgagagc ccccggacaa cccggactcg tggaaacttc gaacaacatg
     2401 gcgaaagccg agattcgcaa cggctgtctc gagatcctga gcagtcagcg ttcactggtc
     2461 atgtcccgcc tcgacgctct gacgcaaaaa ctcgaagcca tcgccagact ctccggcgcc
     2521 acctaccaga gcaacgtcgg gtatccctcc tggcaacccg atttcgagtc cccgttcatc
     2581 agaacctgcg cgggtttgta caagaaactg ttcaagaagg aagcgcgggt cgaggtgatt
     2641 cacgccggtc ttgaatgcgg cattatcggc gcgctgatac ccggcatgca gatggtctcg
     2701 atcggcgtga cgctgcgcga ccctcacagt ccgcgcgagc gcattctcat ttcctcgatc
     2761 gacccggtct ggaagtttct cgtcgcgatg ctggacgatc tcgccaactg acgcgcgcac
     2821 cgcggatctt cgacgcaggc gcttcgtcga ggtcgccgtt cgtcattcga tctcgcgaag
     2881 cccccagggc ggaatagcca ccggtacttt gaactcaaag tacagatcga ggccgcagat
     2941 ctccttgtag gccagacgca tcagatcgcc agtgtcgcct tgttccaggc ggcttttcca
     3001 ttcagcaaac cgagcgggct cagccgtaga aattccggca agataagcct cgatgacctg
     3061 aagcgtcagc gcgcgaaaat cgtcaaccgt gaaatccaat tgtcccagtc ggcgacgcac
     3121 cggacgccag agcggaaagg aatcacgcag catacgattc cagcgcgggt tatcacgttt
     3181 gtagggccgc agacttttca ccagactctg cagatcaccc ccagatgtcg tcatttcccg
     3241 cgggaactcg cgttgaaatg cgggagtcag ttgatcagcc agagtctttt cgctgccaaa
     3301 cgcgaagcga tacagatcat agggatacat cacgaaggca ctgacccagc cacgctcgcg
     3361 tccgaacgtt tcttccggag cgaacttgta cttgccatac agcgactcac gggcattccc
     3421 cagtttcggc ccggactgca gcaacacgaa gaagaagatg ttgtcgtctg aatgcagaaa
     3481 ctggcgattg aagtagtagg tattgagccc gccgatcccg ctgacttcct gcaacacgcg
     3541 tgaactgacc agcggcgtat ccggaaccga gcggatcaca tgagtattgt gaatgaaaac
     3601 gacggaaagt ttttgtacaa gttcacgaac ttcgttcttc tcagttactg ccggaagcag
     3661 gcgagagaca cgcactcgat tgcgcgcgct gatctcatgc agcgtgcgat acccgtcggc
     3721 ggcaaacaat ccgggctctc tgatcggatc gcggcggaaa aacagcgaac gagccatgga
     3781 ttcacgctgt cgcgtttcca gaaacagcgg gtcggaaaat gggcgcagcg actcacgcag
     3841 aaacgcggcc acctggggat aacgctcgtt acgggccaga cgtgaaccat cggccagacc
     3901 gttcgagcgc aggaaggcgg aaaaagcctc gttgcgcaac aggcgctgcc agggttcctc
     3961 agctgcctct ccctgcacgc ggccggattt gtcgagcaga tagcgagcca ggctctcaag
     4021 cgggtcagcc gacccgatct cctcttcatc gtgggtttca gggatcgccg gcatcgtagc
     4081 cggcgacgcg gcagctcccg tcgtcagcat cgcgagtgag agaagtagca gaccgaagat
     4141 ccatgatgaa tggcgcatga ctcgtcccct ttctcgaacc agtgtcaccc tgtgttacca
     4201 tacctgtcga cctttcgtca cggagtcgcc acatgtcgtg ttgttccact ctctccggaa
     4261 ctgtccgcca acggtcccgg tcggtttttc tgctgctcgt tctggcggtg ctgataccct
     4321 tgtgcctgca gggccgtccg tccaagccgg cgtttcgttt cggcaaggcc ccggaacagc
     4381 cgccccgaac cgcgcatctg gtcgtgctcg cgacctctga tgtgcacggc gagctgctgc
     4441 cctacgacga aatcgcc
//
LOCUS       DAORHD010000223         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20889_length_4445_cov_3.467882, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000223 DAORHD010000000
VERSION     DAORHD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20889_length_4445_cov_3.467882"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2346
                     /locus_tag="PKO06_06050"
     CDS             <1..2346
                     /locus_tag="PKO06_06050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNV69242.1"
                     /translation="HDLGREMFLEKGWAWKEKYGGIIINQLKRLGCSCDWQRERFTMD
                     EGLSKAVQECFIRLYRDGLIYKGKYIINWCPRCRTALSDEEKIPEEKKSHLWHIRYPL
                     KSGKGHVIVATTRPETMLGDTAVAVNPDDDRYRDLVGQTLILPVMNREIPIIADDFVD
                     PKFGTGAVKVTPAHDPNDFEMGRRHQLPQVVVMDETGKMNEHAGKYQGQDRFKCRENL
                     VEELKELQLLEKIEDHTHAVGHCERCSTVIEPYLSDQWFVRMKPLAQKAIEAVKTGKL
                     RFTPDRWVGVYLHWMENIRDWCISRQLWWGHRIPAYTCQACGHVMVDRAVPASCEKCQ
                     TAGKIEQDNDVLDTWFSSWLWPFSTLGWPDDTQALRKFYPTDTLITGPDIIFFWVARM
                     VMAGYHFVGECPFHDVYFTSIVRDLKGRKMSKSLGNSPDPLDIMAKYGADALRFTITF
                     LAPVGQDIRFAAEQVEIGRNFANKLWNASRFVLMNLGDAENYSLKKLPIQNSDASTWD
                     RWILSRLMNAVDLVHKYLADGQYKFNESIKAVYEFIWDEFCDWYIEMSKATLLGPDGP
                     AKEAVRQTLVRVLDTALRLLHPFMPHITEEIWQKLPGAEGMLIRQTFPKVQAELVNLP
                     LETEVTEVMEAIRVVRNLRASANIQPAKKVTVRVIEGARSDVFKRYGSMIQALAGVET
                     LEIVSTKPGGHLVGLFGETEVCLNLAGIIDVAVEIPRIEGEVAKIEKERVKIAQKLEN
                     QDFVTRAPVEVVDKIREGITAYDSQLVKLKEYLAELKKVAS"
     gene            2346..3563
                     /gene="tyrS"
                     /locus_tag="PKO06_06055"
     CDS             2346..3563
                     /gene="tyrS"
                     /locus_tag="PKO06_06055"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNV69243.1"
                     /translation="MFTATPKEQFDVLTRGAADVVSREDLLKKLEKSYATKKPLVVKL
                     GADPSAPDIHLGHTVVLQKLHQFQMFGHEVVFLIGDFTGRIGDPTGKKKTRPALTEDE
                     VMANAETYKQQIGKILDLGRTRIVFNSHWLGKMTFADVLKLTSQYTVARMLERDDFST
                     RYKNQVPISVVEFMYPLMQGYDSVALKADVELGGLDQTFNLLVGRELQRSYGQEPQIT
                     LTLPILEGLDGKEKMSKSLGNYIGINETPDQIYGKAMSIPDELMGRYYDLLTDYSTQE
                     LQAIKARMTTEPKKVKMQLAKHLTAKYHGANAGEQAEQGFEKLFGKTGDGLPENMEEC
                     RVEAGSEGLTLLKLLVQTGLSPSGGEARRMVQQGGVKVDQEKQADAQRLFKAGETFVL
                     QAGKRGFRRITLV"
     gene            complement(3618..4166)
                     /locus_tag="PKO06_06060"
     CDS             complement(3618..4166)
                     /locus_tag="PKO06_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV69244.1"
                     /translation="MEDIKRLEEHFTDCSVESGSETGIQFKIACYRCKRSWESEFIPK
                     TKSGGVVSKFFKKDSANPVDTEWEKAKAAALTSAAEQVEDAFKFCPRCEHFVCLKCWN
                     AYREMCLECVAGISRAALEEAYNKSHEEVEEKCKKCGTVVTKMKFCPKCGEKVSRGEC
                     PGCTKKLPPEAAFCPECGYKVK"
BASE COUNT         1074 a         1166 c         1252 g          953 t
ORIGIN      
        1 cacgatctgg ggcgagaaat gttcctcgag aaaggctggg cctggaagga aaagtacggc
       61 ggcatcataa tcaatcagct caagcgcctt ggctgctcat gcgattggca gcgcgagcgc
      121 ttcaccatgg atgaagggct ctcgaaggcc gtgcaggaat gtttcattcg cctgtaccgg
      181 gacggcctga tctacaaggg gaaatacatc atcaactggt gcccgcgctg tcgcacggct
      241 ctgtctgatg aagaaaagat tcccgaagag aaaaagtccc atctgtggca catcagatat
      301 ccgttaaaga gcggaaaagg gcatgtcatc gtcgccacga cgcgccctga aaccatgctc
      361 ggcgataccg ccgtggcggt gaaccccgac gatgatcgct atcgtgatct ggtcgggcag
      421 accctgattc tcccggtgat gaaccgcgag attcccatca tcgccgatga tttcgtcgac
      481 ccgaagtttg ggaccggggc cgtgaaagtt actccggctc atgatccgaa cgattttgaa
      541 atgggacgca ggcaccagtt gccacaggtt gtggtcatgg atgaaaccgg caagatgaac
      601 gaacatgccg gaaaatacca gggccaggac cgcttcaagt gccgcgaaaa cctggtcgag
      661 gaactgaaag agttgcagct cctcgaaaaa atcgaagatc acacccacgc ggttggccac
      721 tgtgaacgat gcagcacggt tattgaaccg tatctttccg atcagtggtt cgtccgcatg
      781 aagccgttgg cgcagaaggc gattgaagcg gtgaagaccg gcaaactgcg atttacgccc
      841 gatcgctggg tcggagtcta tctccattgg atggaaaaca ttcgcgactg gtgcatcagc
      901 cgccagttgt ggtgggggca tcggattccc gcctatacgt gtcaggcgtg cggtcatgtc
      961 atggtcgatc gtgccgttcc tgcctcttgt gaaaagtgtc agaccgccgg caaaatcgaa
     1021 caggacaacg atgtgctcga tacctggttt tcttcatggc tctggccgtt ttctacgctg
     1081 ggctggcccg atgataccca ggcgttgcgg aaattctacc ccaccgatac gctgatcacc
     1141 ggccccgata ttatcttctt ctgggtcgcg cgcatggtga tggctggcta ccattttgtc
     1201 ggggagtgcc cgttccacga tgtctacttc accagcatcg tgcgcgacct caagggacgc
     1261 aagatgagca agtcgctggg gaactcgcct gatccgctcg acatcatggc gaagtatggg
     1321 gcagacgcac tgcgctttac catcaccttc ctggcaccgg tcggccagga cattcgcttt
     1381 gccgcggaac aggtggagat cggccggaac tttgccaaca agctctggaa cgcatcccgc
     1441 ttcgtgctca tgaatcttgg tgatgccgag aactattcgc tgaaaaagct gccgatccag
     1501 aattcagatg cctcgacctg ggaccgctgg atcctgtcac gtctcatgaa tgccgtcgat
     1561 ctggtgcaca agtatcttgc cgacggccag tacaaattca acgagtcgat caaagccgtc
     1621 tatgagttca tctgggatga gttctgcgac tggtacatcg agatgtcgaa agcgacgctg
     1681 ctcggtcctg atggcccggc caaagaagcc gtgcgtcaga cgctggtgcg agttctggat
     1741 acggcgttgc gtctgctgca tccctttatg ccccacatta ccgaagaaat ctggcagaaa
     1801 ctgcccggcg ctgagggcat gttgatccga cagacgttcc cgaaagtcca ggctgagctc
     1861 gtgaatctgc cgctcgaaac cgaagtgacc gaggtcatgg aagccattcg tgtagtgcgc
     1921 aatctgcgtg cttccgcgaa cattcaaccg gccaagaaag tgaccgtccg agttattgag
     1981 ggtgctcgca gcgatgtgtt caaaagatac ggaagcatga ttcaagcact tgccggagtc
     2041 gaaaccctcg agatcgtctc gacgaaaccg ggtggtcatc tggtgggcct gttcggagaa
     2101 accgaagtgt gcctgaatct cgccggtatc atcgacgtgg ctgtcgagat tccacgcatc
     2161 gaaggcgaag tcgcgaagat cgaaaaagaa cgggtgaaga tcgcgcagaa actcgaaaac
     2221 caggatttcg tcacccgtgc gccggttgag gtggtcgata agattcgcga aggaatcacg
     2281 gcgtatgact cgcagttagt gaaactgaaa gagtatctgg cggaactgaa aaaggtggct
     2341 tcgtgatgtt tacggccacc ccgaaagagc agttcgatgt tttgacccgt ggcgcggctg
     2401 atgtcgtgtc acgcgaagac ctgctgaaga aactcgaaaa atcgtatgcg acgaaaaaac
     2461 cgctggtcgt aaaactcggt gctgatccgt ctgccccgga tatccacctg ggacacacgg
     2521 tcgtgctgca gaagctgcac cagtttcaga tgttcgggca cgaggtcgtt tttctcatcg
     2581 gagattttac cgggcgaatc ggcgacccga cgggcaagaa gaagacgcgc ccggccctga
     2641 ccgaagacga agtcatggcc aatgccgaaa cgtacaaaca gcaaattggt aagatccttg
     2701 atctgggccg cactcgcatc gttttcaaca gccactggct ggggaagatg accttcgccg
     2761 atgtgctcaa gctgacgagc cagtacaccg tggcacgcat gctcgaacgc gatgattttt
     2821 ccacccggta caagaaccag gtgccaatca gtgtggtcga gttcatgtat ccgctgatgc
     2881 aggggtacga ttcggtggcc ttgaaggcgg atgtcgaact tggcgggctc gaccagacat
     2941 tcaatcttct cgtggggcgc gagttgcagc gctcgtatgg ccaggaacct caaatcaccc
     3001 tgaccctgcc gattctcgaa ggtctcgatg gcaaagagaa aatgtcgaag agcctcggca
     3061 actacatcgg tatcaatgaa actcctgatc agatctacgg aaaggcgatg tcgatcccgg
     3121 acgaactgat ggggagatac tacgacttgc tgaccgacta ttccacccag gaactgcagg
     3181 cgatcaaggc gcgcatgact accgagccga aaaaggtgaa gatgcaactg gccaagcacc
     3241 tgacagccaa gtatcacggt gccaatgccg gggaacaggc tgagcagggg tttgaaaaac
     3301 tgttcggcaa aaccggcgat ggattacccg agaacatgga agagtgccgt gtcgaagccg
     3361 gttctgaagg ccttactctg ctgaagctgc tggtacaaac cggcctgtcg ccatcaggtg
     3421 gagaagcccg acgcatggtg cagcagggtg gggtaaaggt ggatcaggaa aagcaggccg
     3481 atgcccagag gctgttcaaa gccggcgaaa ccttcgtgct gcaggcaggc aaacgcggtt
     3541 ttcgtcgcat caccctggtc tgatagaaag cagacaaaaa aaccggcctt cgacaggccg
     3601 gtttttttta aggtgtgtta cttgaccttg tagccgcact ccggacagaa tgccgcctcg
     3661 ggcggcagct tcttggtaca accggggcat tctccgcgtg acaccttttc accgcatttc
     3721 gggcaaaact tcatcttggt gacgacggtg ccgcactttt tgcatttttc ttcgacttcc
     3781 tcgtgagact tgttatacgc ctcttcgaga gccgctcgcg aaattccggc cacacactca
     3841 aggcacattt cccgataggc attccagcac ttgaggcaga cgaaatgctc acatctgggg
     3901 cagaacttga acgcgtcttc gacctgctcg gcagcagaag tgagtgcggc agctttagct
     3961 ttttcccatt ctgtatcgac cggattcgcg ctatcttttt taaaaaactt gctgacgaca
     4021 ccaccagact tggttttcgg gataaactcg gattcccagg agcgcttgca ccgatagcag
     4081 gcgattttga actgaatacc ggtttctgaa ccagactcaa ccgaacagtc ggtgaagtgt
     4141 tcttccagtc gcttgatgtc ttccatagcg ggtcctctac tcagttacga cgttcttgtg
     4201 gctaaatacg ataatacata tatcatattt cggaacaatg cacgaaaaac ctttttcaaa
     4261 cttttctgtg aacgtggttg caggcgttga gcggcagtga ctgagatatc ttgtctgcac
     4321 ggaaaatgcg cttgagatgc ttgttcgcag cagggcgcaa cttgacaaaa ccactgaaaa
     4381 tgaggagaat ggacgacaat tacgaacgga aggccgacaa ggagcataca tgtcacagaa
     4441 tatca
//
LOCUS       DAORHD010000224         4440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20916_length_4440_cov_3.952566, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000224 DAORHD010000000
VERSION     DAORHD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20916_length_4440_cov_3.952566"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PKO06_06065"
     CDS             <1..574
                     /locus_tag="PKO06_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HNV69245.1"
                     /translation="QTGAIGIAAWCSTMAAALACAGQLATSGVIAWNTVFPAMGGIHA
                     LIGIGEALITVLVIRAVSRAHPDLQNISNLVTEGDSRSLRLYGVVAAVGLVLFISPFA
                     CSWPDGLEKIAGDLGFEHQAAKVPVFPAPIPDYELPGIESDSGKTILAGLVGLAATLA
                     VFSLIGFSVKRLRSPALPATASEPTRSLAD"
     gene            564..1169
                     /locus_tag="PKO06_06070"
     CDS             564..1169
                     /locus_tag="PKO06_06070"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HNV69246.1"
                     /translation="MLTDPFQPGDSPIHRLDPQLKLATLLAVIVATALLPSGSWRLFL
                     GNALLFLLVLSFSGLSVWRLTRQFLVLEAPACGFALFALLHENGHILFFTLLCKSSLC
                     LLWVLLMTATTPFSTILTAARRLKLPRLLVSTLTLAWRYLFVLSEEAERLERARRSRT
                     LNAGTRLTWFLNTDVIGNLFVRTLFRAERVFAAMSARGWKR"
     gene            1166..1915
                     /locus_tag="PKO06_06075"
     CDS             1166..1915
                     /locus_tag="PKO06_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HNV69247.1"
                     /translation="MNALEVRNVCYDYPDGTRALRGISFTLAEGERLGLIGPNGAGKS
                     TLLWHLNGLLPDPLTQASSVTVFDLPMLPSHLPLIRARVGLLFQNPDDQLFCPTVADD
                     VAFGPQQLGLSSTEIDARVKTSLSQVALPGFEPRHPHRLSHGEKHRVALAGLLAADAR
                     LLLLDEPSAGLDPRGRRELQSLLRSLPQAQIIATHDLELVVALCQRVLLLNQGQVVAC
                     GPTRDLLNDEPLMLANGLERPHCLRHPHPHD"
     gene            complement(1928..2476)
                     /locus_tag="PKO06_06080"
     CDS             complement(1928..2476)
                     /locus_tag="PKO06_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69248.1"
                     /translation="MIRNEALKLLQLHLSQDSLIKHSLATEAIMRALATRFSENADRW
                     GLLGLLHDLDFESTKDNMALHGKKTVELLGQTLDNVERQAILSHNEMTGFVRASRLDH
                     ALAAAESVTGLIAATALIYPDKKLTSVKVSSVIKRMKQPAFARAVSRETIMECEKIGL
                     TLDEFITLSIEAMKSVADDLGL"
     gene            2574..>4440
                     /locus_tag="PKO06_06085"
     CDS             2574..>4440
                     /locus_tag="PKO06_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69249.1"
                     /translation="MPMLSPLRWLSLLLALLPLWFGNWAIDFLEHRQVAWQEQRQEDR
                     ARRELEGMRARVEFPRRLEWCAGKLESLIGEATERGIIGESLATQLERLQMQLLGTPD
                     LPTAGFLVRFGPGGSDDQLTHWGQLSEAVPRRVVQRILRSLHALSTQPSPVADSSVDR
                     LLEELFGRGTRSEIIALSQQGIATPVLFQRKQYWLLWSPVRTQSEIVGGLLLLVPNDD
                     TTSSLGYALTMRQKRNARRRCGFVRVMPSRAGDQLSRSLQQSGAFHAWLTRWRQTATP
                     ESIEVHGWPWAVPIGDARLFSAPIMPNGTHLAMLLLPRPSLSTEKGRGRFLLNWMLFL
                     AVVLLWYHEPTWRKAWRIPLGWRFLGLFLFATLMPVFQLMIAVSAYLQENELTLQSRL
                     RSHLAQTLQKFDDAKDRLRSAYCAAFTRVLNDTELKLHLQHHGLNDSHLLNLVTRHFR
                     QGPVSLPLLAVSVFDIHASGPVSLGRRLPEGFTRQVRFYRYSLAQSHRESLGSAATDL
                     PPLQIPEEVQAAMDCYEGSSGSRFTGEMEIQRSIPMNQSIGRNSSMMMHDRLAIGGSD
                     RYTLLILWNDTEMDREILAQTCSEIRIEAPNLLLGLYRNAGDHLTSILPEDQHHPA"
BASE COUNT          770 a         1426 c         1254 g          990 t
ORIGIN      
        1 acaaaccggg gccatcggca tcgccgcctg gtgctcgacg atggccgcgg ctcttgcctg
       61 cgccggacag ttggcaacgt ccggagtcat tgcctggaat accgtgtttc cggctatggg
      121 cggcattcat gccctgatcg gcatcggcga agctctgatc acggttctcg tcatcagagc
      181 cgtcagccgt gcccaccccg acctgcagaa catttcgaac ctggttaccg aaggcgattc
      241 acgttctctg agattgtatg gggtggtcgc cgcggtcggt ctcgtactct tcatctctcc
      301 cttcgcctgt tcatggcccg acgggctgga aaagatcgcg ggtgacctcg gcttcgaaca
      361 ccaggccgcg aaggtgcccg tcttcccggc accgattcct gattatgaac tgccgggcat
      421 cgagtctgat tccgggaaaa cgattctcgc cggtttggtc ggcctggccg ccactcttgc
      481 cgttttcagt ctgatcggct tcagcgtgaa acgtctgcgt tcgccagcac ttccggccac
      541 ggcttccgag cccacgcgca gccttgctga ctgatccgtt ccagcccggt gacagtccga
      601 ttcaccgact ggacccgcag ctgaagctcg ctaccctgct ggcggtgatc gtcgccacgg
      661 cccttctgcc ctccggctcg tggcggctgt ttctcggtaa cgccctgttg tttctgcttg
      721 tcctctcgtt cagcggctta tctgtgtggc gtctcacccg gcagtttctc gtccttgaag
      781 cgcccgcctg cggctttgcg ctgttcgccc ttctgcacga aaacggccat atcctgttct
      841 tcacgctctt gtgcaaaagc tctctctgcc tgctctgggt tctcttgatg accgcgacca
      901 ctccgttttc caccatcctg actgccgcgc gtcgactgaa actgccccgc ctgctcgtca
      961 gcactctgac gctcgcctgg cggtatcttt tcgtgctttc cgaagaagcg gaacgactgg
     1021 agcgcgctcg gcgcagtcgc accctgaatg ccggaacccg cctcacgtgg ttcctgaaca
     1081 ccgacgtcat cggaaacttg tttgtacgaa cgctctttcg tgccgaacga gtttttgccg
     1141 ccatgtcggc cagagggtgg aaacgatgaa cgcattggaa gtgcgcaacg tgtgttacga
     1201 ctatcccgac ggtacaaggg ctttgcgcgg tatatccttt actttggccg aaggagagcg
     1261 actcggactg atcggcccga atggtgccgg aaaatcaacc ctgctctggc atctgaacgg
     1321 gctgctgccc gacccgctga cgcaggcaag ttcggttacg gttttcgatc tgccgatgct
     1381 gccatcacat ctgcctctga ttcgcgcccg ggtcggactt ctctttcaaa accccgacga
     1441 tcagctgttc tgtccaaccg tggctgacga tgtcgctttc ggcccgcaac aactcggact
     1501 ctcatcgacc gagatcgacg cacgcgtgaa aacctcgctg tcccaggtcg ccctgccggg
     1561 cttcgagcca cgacacccgc atcgtctgag ccacggggaa aagcatcggg tggcgctcgc
     1621 cgggttgctc gccgccgacg cacgcctgct tctgcttgac gaaccttcag cgggcctcga
     1681 cccacgcggc cgccgcgaac tgcagagtct gttgcggtcg ctgccgcagg ctcagatcat
     1741 cgccacgcac gatctcgaac tcgtcgtcgc gttatgtcag cgcgtgctgt tgctcaatca
     1801 gggccaggtc gtcgcctgcg gcccgactcg cgatcttctg aacgacgagc cgctgatgct
     1861 ggccaacggg ctcgaacgcc cacattgcct gcgccacccc cacccgcacg actgagaaga
     1921 ggatcaacta caaccccaga tcatcagcga cagatttcat cgcctcgatc gacagggtga
     1981 tgaactcatc gagcgtcagg ccgatttttt cgcattccat gatggtttcg cgactgacgg
     2041 cgcgggcgaa ggcgggctgc ttcatgcgct tgatgaccga agagactttc acgctggtca
     2101 gctttttgtc ggggtagatc agcgcggtgg cagcaatgag tccggtcacg ctttctgcgg
     2161 cggcaagcgc gtgatcgagc cggctcgctc gcacgaaccc ggtcatctcg ttgtgcgaca
     2221 gaatcgcctg tcgctcgaca ttatcgagag tttgtccgag cagctcgacc gtcttcttcc
     2281 cgtgcagagc catgttatct ttggtcgatt caaagtcgag atcgtgcagc agccccagca
     2341 gtccccaccg atctgcgttt tcgctaaacc gggtcgccag tgcccgcatg atggcctcgg
     2401 tggccaaaga gtgcttgatc agggaatcct gactgaggtg cagttgcagc agtttcaggg
     2461 cttcattacg gatcatcgtc ggttctcccg gggtgagggt ttgttcctcg tcttcaggct
     2521 caccctgctc accgatgata tcacgcccac ccgacgcgtc gtaatgatac aatatgccta
     2581 tgctttcccc gctgcgctgg ctatcgctgc tgctcgccct gctcccactc tggttcggca
     2641 actgggccat cgattttctc gagcaccgtc aggtcgcctg gcaggagcaa cgacaggaag
     2701 atcgcgcccg ccgggaactg gagggcatgc gcgcacgggt cgagtttcca cgccgcctgg
     2761 aatggtgcgc cggcaaactg gaatctctga ttggcgaagc tacggaacgc ggcatcatcg
     2821 gcgaatctct ggcgacccaa ctcgaacgtc ttcaaatgca gcttctcggc acacccgacc
     2881 tgcccacggc cggattcctg gtcaggttcg gcccgggcgg cagcgacgat cagctcaccc
     2941 actggggtca gctgtccgaa gccgtgcctc gacgggtggt ccagaggatt ctccgttcgc
     3001 ttcatgcgct ctcgacacag ccatctcctg tcgctgacag ctctgtcgac cgattgctcg
     3061 aagagctgtt cggccgcgga acccgcagtg aaatcatcgc tctgagtcag cagggaatcg
     3121 ccacccctgt tttgtttcaa cgaaaacagt actggttact ctggagtccc gttcgcacac
     3181 agagtgagat tgtgggcggt ctccttctgc tggtgccgaa cgatgacacc acgagcagtc
     3241 tcggatatgc gctgaccatg cgacagaagc gcaatgcccg ccgccggtgc ggttttgtcc
     3301 gggtcatgcc gagccgagcc ggtgatcaat tgagccgttc gctccagcag tctggcgctt
     3361 ttcacgcctg gctgacccgg tggcgtcaga ccgcaacccc cgaaagcatc gaggttcacg
     3421 gctggccctg ggcggttccc atcggtgacg cgcgcctttt ttccgcgccc atcatgccga
     3481 acggcacgca tctggccatg ctgttgctgc ctcgcccatc gctttcgacc gagaaaggtc
     3541 gtgggcgttt cctgctcaac tggatgctgt ttctggcggt ggtgctgctc tggtatcacg
     3601 aaccgacctg gcgcaaagcc tggaggatac ctctgggttg gcgatttctc ggtctctttc
     3661 tcttcgcgac gctcatgccc gtgtttcagc tgatgatcgc cgtttccgcc tatctgcagg
     3721 aaaatgaact gacgctgcag tctcgcctgc gctctcatct ggcacagacg ctgcagaagt
     3781 ttgacgacgc gaaggatcga ctcagatcgg cctactgtgc cgccttcact cgggttctca
     3841 atgacaccga gctgaaactg cacctgcaac atcacgggct gaatgattca catctgctga
     3901 atctggtgac ccgccacttc cgtcagggcc cagtttctct gccgttgctg gccgtctcgg
     3961 ttttcgatat tcatgccagc ggccccgtct cgctcggcag acggcttccc gagggcttta
     4021 cacggcaggt acgcttctac cggtacagcc tcgcgcagtc gcatcgcgag agcctcggtt
     4081 cggccgccac cgatctgcca cctctgcaga ttcccgaaga agtgcaggcg gcgatggatt
     4141 gctacgaggg ttcttcgggc agtcgcttca ccggagagat ggagattcaa cgctcgatac
     4201 ccatgaacca gagtatcggt cggaacagtt ccatgatgat gcatgaccgt ctggctatcg
     4261 ggggtagtga tcgctacacg ctgctgattc tgtggaacga cacggaaatg gatcgagaga
     4321 ttctggcaca aacctgcagc gagattcgca tcgaggctcc caatctgttg cttggcctgt
     4381 atcgcaacgc tggggaccac ctcacctcga ttctgcccga agaccagcat cacccggccg
//
LOCUS       DAORHD010000225         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20937_length_4437_cov_3.453902, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000225 DAORHD010000000
VERSION     DAORHD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20937_length_4437_cov_3.453902"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2452
                     /locus_tag="PKO06_06090"
     CDS             <1..2452
                     /locus_tag="PKO06_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV69250.1"
                     /translation="FDGRVRQTLDATDFQKRFARGEEWERQLRGIDREPETNLGEALE
                     QSTRLVPAGQRGQVVLLSDGRDTAGTWEDAASRLGRSNVPVITMPLTSQAMKRDTAIA
                     RLIVPEQCFMGEEIQGRVLLRCRSEQTLDVTVALGATKPERQKVQVVEGLTAHSFSLK
                     PAKVGRQILEVRAESTAAPGSIVTVRALIQVKPLPRALIIESDEVSGRFLRDVLRQEG
                     VDYLSYSPASAPQELLAEIRNYPCLILNNIPKTVFQDKHQIAIREAVREGMGLLMVGG
                     VRSFGLGEYTDTPIEEALPVRMPRVTVNQPLALVLLLDASGSMFNEPWSYLKEAAKEV
                     VRLCRGHVLGIVVFNNYPYWVWPLSQIDDPEKVCYLLDQVGVGGGTIFSLPMAEGYSA
                     LKKTAFEKKTVILFSDGIPADFQAAESLLDYFRQERITITTIGAGNNINPMVLETIAN
                     ETGGQFHSSPDFADLPKLFREEFKRLVGPPIAEADMKPVLKSGAGAVRGFRPDEFPEV
                     SGINVTQLKPRAEELLVTPNGDPLLVQWRFGLGRGMALTTDLLPNWSSRWARWDGFGK
                     LMRQSIKELSEGARDPWQVQVNQTGATVRVDVNYIGDRKRRPVMLHVDRLPGGATSVP
                     FRDGRNGRYTAEFRCLTPGVYPAHISGPDGALPGDLPLPVNESEEWAEGITDFERLRQ
                     IAERTGGEFWETAWAEPLHPPVTGAEVRLFSPLWPFFAVCALLLYLVDIFLRKAGIFS
                     ITRRSESQLSEESGEDLYRQLAVKFSTLAEEAALKGNEEEAKQFFLRAKAFYLKGQAQ
                     REAQLMWERYKRFDRGNP"
     gene            complement(2453..3460)
                     /locus_tag="PKO06_06095"
     CDS             complement(2453..3460)
                     /locus_tag="PKO06_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV69251.1"
                     /translation="MKTPESSTTPPRIPLEQLKKEIGKAIVGSDAVIEQVLICLFAGG
                     HGLLEGVPGLGKTMLVRSLASILQLGFRRIQFTPDLMPADIVGTKLIQEDESGRKVFV
                     FQPGPLFANLVLADEINRATAKTQSALLEAMAEATITAAGETYSLQKPFFVLATQNPI
                     EMEGTYPLAEAQVDRFLFKIQVDPPDQATLESILELTTTNTVPELSKLLNAEQIIAAM
                     RLVRDVPIAKHLREAIAALIVATQPKQASAPQPVKRFIAYGSSPRGLQSVALAAKSLA
                     YLRGHNHVSFDEIKAVAKPALRHRLLLNFEGEAEGISTDEIIDKIVTQIESSLAIGKP
                     A"
     gene            complement(3447..>4437)
                     /locus_tag="PKO06_06100"
     CDS             complement(3447..>4437)
                     /locus_tag="PKO06_06100"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV69252.1"
                     /translation="MGLVHLFMNLLALKYFGPPLETVIGAPLLLGVFLFSGICGSLLS
                     VWQLTGLSVGASGAVLGLLSAAIMLEWIGGSAAKKLARYENLGTLLFILGVNLLIGTV
                     EKGIDNSAHIGGLIGGAIAGLAAAVIIRVPVATRLAEALVIVISCFTLGWAGHQFATV
                     ETSTQYPANLRSLKPLQHSTLPLNLALPDTWVSRLSSDTGTLQELSVQGAFGERLDIS
                     WGLNAEPPEQWVRTYAEARTRTIAGDELVRLKAVSDPATSTVGIWQVFRIQWRLAAQA
                     HPLVIRDYALFHHREMVLIQCLIPTELAHLYDSLFQKIIESITFKKENIDEDP"
BASE COUNT          937 a         1184 c         1382 g          934 t
ORIGIN      
        1 cttcgacggt cgtgtccggc agacacttga tgccaccgac tttcaaaaac ggttcgcgcg
       61 tggcgaagag tgggaaagac aactgcgcgg tatcgatcgc gaacctgaga ccaatctggg
      121 tgaggctctc gagcaatcaa ctcgactggt gcccgccggg cagcgtgggc aggtggtttt
      181 gctttctgat ggtcgtgata ccgctggtac atgggaagat gctgcttcgc gactcggacg
      241 ttcgaatgtg ccggttatca ccatgcctct cacaagtcag gccatgaaac gtgatacggc
      301 gattgcccga ctgattgtcc ccgaacaatg ttttatgggt gaagagattc aggggcgggt
      361 actgctgcgc tgccgcagcg agcagacact ggatgtgact gtcgcgcttg gcgcgaccaa
      421 gccggaaagg cagaaagtac aagtcgtgga aggtctcact gcccattctt tctcattgaa
      481 gccggcgaag gtcggtcgtc agattctgga agtgcgcgcc gagtcaactg ctgctcccgg
      541 gtcgattgtc acggttcgag cgctcattca ggtgaagccg ttaccgcgtg cgctgatcat
      601 tgaaagcgac gaggtgtcag ggcgattcct gcgtgatgtc ctgcgtcagg aaggggtgga
      661 ctatctcagc tactctcccg cgtcagcccc gcaggaactt ctggcggaga ttcgcaatta
      721 cccctgcctg atcctgaata acattcccaa gacggtgttt caagacaagc atcagatcgc
      781 tatacgtgaa gccgtgcgtg aaggcatggg tctgctcatg gtcggcggtg tgcggtcgtt
      841 cgggctcgga gagtatacgg atacgcccat cgaagaagcg ttgccggtgc ggatgccgcg
      901 cgtgacggta aatcagcctc tcgcgctggt attgctgctc gatgcctcgg gaagcatgtt
      961 caacgaaccc tggtcctatc tgaaggaagc cgctaaggaa gtagtacgct tgtgtcgcgg
     1021 tcatgttttg gggatcgtcg tgttcaacaa ctatccctat tgggtttggc ctctgtcaca
     1081 gatcgacgat ccagaaaagg tgtgctacct cctcgatcag gtgggggtcg gtggcggaac
     1141 catcttctcg ctgccgatgg ctgaaggata ctcggcgctc aagaagacgg cctttgagaa
     1201 gaagacggtg attctgtttt ccgatgggat tccagcagac tttcaggcgg ccgagagcct
     1261 gctggactat ttccgtcagg aacgcatcac gatcaccacc attggcgcag gcaacaacat
     1321 caatccgatg gtgctggaaa ccatcgccaa cgagaccggt gggcagtttc actccagccc
     1381 cgactttgcc gatctgccga agctgtttcg cgaagagttc aagcgtctgg tcggtccgcc
     1441 gatcgcagaa gcggatatga agccggtctt gaaatccgga gctggcgcgg tgagggggtt
     1501 tcgacccgac gagtttcccg aggtgtcggg gatcaacgtg acgcaactga agccacgtgc
     1561 cgaagaactg ctggtcacgc cgaacggcga tccgctgctg gttcaatggc ggtttggact
     1621 cgggcgtggg atggcactga cgacggatct gctgcccaac tggagctccc gctgggctcg
     1681 ttgggatggc tttggcaaac tcatgcgcca gtctatcaaa gaactctcgg aaggggcgcg
     1741 agatccctgg caggtgcagg tcaatcagac tggagcgaca gtacgagtcg atgtgaacta
     1801 catcggtgat cgaaagcgaa gacctgtcat gttgcatgtt gatcgcctgc ccggtggcgc
     1861 gacctcggtt ccctttcgag atggccgaaa cggccgatac acagcggagt ttcgctgtct
     1921 gacgccgggc gtttatcccg cccacatttc cggtccggat ggggcgttgc cgggggacct
     1981 gcctcttccg gtgaatgaat ctgaagaatg ggctgaggga atcacggact tcgaacgatt
     2041 gcgtcagatc gccgagcgca ccggtggaga gttctgggaa accgcctggg ccgaaccgct
     2101 ccacccgccg gttaccggag cggaagtgcg gttgttttcg ccactctggc cattctttgc
     2161 cgtctgcgcg ctgctgctct acctggtcga catcttcctg cgcaaagcgg ggatcttcag
     2221 tatcacgcga cgttcagaaa gtcagttgtc tgaagagagc ggtgaagatt tgtaccggca
     2281 gttagccgtg aaattctcga ctctggccga agaagccgcc ctaaaaggga acgaagaaga
     2341 agccaagcag tttttcctgc gggccaaggc gttctatctg aaaggccagg cgcagcgaga
     2401 ggcacaattg atgtgggaac ggtacaagcg ctttgatcgc ggaaacccgt aattaggcgg
     2461 gtttgccgat cgcgagagac gattcgatct gagtgacgat cttgtcgatg atctcgtcgg
     2521 tcgagattcc ttcggcttca ccttcgaagt tcaacaaaag acggtgcctc agagccggtt
     2581 tggcaacggc tttgatctcg tcgaacgaca catggttgtg gccacgcaga tacgccagcg
     2641 atttggcggc cagtgccacc gactgcaggc cgcgaggcga actgccatag gcgatgaagc
     2701 gtttgaccgg ttgcggggcc gaagcctgtt tgggttgcgt ggcgacgatg agcgccgcaa
     2761 tcgcttctcg caggtgtttg gcaatcggca catcgcgcac caggcgcatg gcggcgatga
     2821 tctgttccgc gttgagcagc ttcgagagtt ccggaaccgt gttggtcgtg gtcagttcga
     2881 gaatcgattc cagcgtggcc tgatcaggtg gatcgacctg aatcttgaac aggaagcgat
     2941 cgacttgggc ttcggccagt ggatacgtac cttccatttc gatcgggttc tgcgtggcga
     3001 gcacaaagaa gggtttctgc agagaatacg tttctcccgc ggccgtgatc gttgcctccg
     3061 ccatggcttc aagcagagca gactgggttt tggcggtggc gcgattgatt tcgtctgcca
     3121 ggaccaggtt ggcgaacaac ggcccgggtt gaaacacgaa cacctttctg ccggattcgt
     3181 cttcctgaat caacttggtg ccgacaatgt cggccggcat gaggtcgggg gtgaactgaa
     3241 tgcgcctgaa cccaagttgc agaatcgaag ccagactgcg tacgagcatg gtttttccca
     3301 gcccggggac gccctcgaga agtccgtggc ccccggcgaa gagacagatc aacacctgtt
     3361 cgatgacggc gtcgctgcca acgatggctt tgccgatctc ctttttcagt tgttcgagcg
     3421 gaatccgggg cggggttgtg gatgactcag gggtcttcat cgatgttttc cttcttgaac
     3481 gtgatcgatt cgatgatttt ctggaacagc gaatcgtaca gatgagccag ttcagtggga
     3541 atcaggcatt gaatcaggac catttcgcga tgatgaaaca gggcgtaatc gcgaataacc
     3601 agcgggtgtg cctgggcggc gagtcgccat tggatacgaa aaacctgcca gattccgacc
     3661 gtcgaggttg cggggtcgct gaccgctttc aaccgcacca gttcatctcc ggcaatggtt
     3721 cgcgtgcggg cttcggcgta tgtgcgcacc cattgttccg gtggttcggc attcaaaccc
     3781 caggagatat cgagtcgttc gccgaaagcg ccttgtacgg agagttcctg gagcgttcct
     3841 gtatccgaag acagacgaga gacccaggta tccggaaggg cgagattcaa cggcagggtc
     3901 gagtgctgca agggcttgag ggaacgcaga ttggccggat attgcgtgct tgtctcgacc
     3961 gtcgcaaact gatgacctgc ccaacccagc gtgaagcacg agatgacgat gaccagcgct
     4021 tccgcgaggc gagtggccac aggcacacgg atgatgaccg cggcggcaag gccggcaatc
     4081 gcgccaccaa tcagacctcc aatgtgcgca ctgttgtcga tgcccttctc gacggttccg
     4141 atgaggaggt tgaccccgag gatgaacagc agggtgccga gattctcata ccgggccagt
     4201 ttcttggccg cggagcctcc gatccattcg agcatgatcg cggcagagag aagcccgaga
     4261 acggcacctg atgctccgac agaaagcccc gtgagctgcc agacagaaag caggctgccg
     4321 cagataccgg aaaacagaaa cacgccgagc aagagagggg caccaataac cgtttccagc
     4381 ggcggaccga aatacttcaa tgccagcaga ttcatgaaca ggtgcaccag acccatg
//
LOCUS       DAORHD010000226         4437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_20938_length_4437_cov_3.385212, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000226 DAORHD010000000
VERSION     DAORHD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20938_length_4437_cov_3.385212"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..597
                     /gene="hslV"
                     /locus_tag="PKO06_06105"
     CDS             55..597
                     /gene="hslV"
                     /locus_tag="PKO06_06105"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNV69253.1"
                     /translation="MTHEPLMHGTTILAVLRDGKLAMAGDGQVTLGQTILKARAHKVR
                     RLHGGKVLGGFAGHTADAFTLFERFEAQLEKASGNLKKAAVDLAKDWRLDRALRRLEA
                     MLLVGNHDQILLISGQGDVVEPDEPIAAIGSGGMYALAAAKALFRHTTLDAETITRQA
                     LGIAAEQCVYTNANLSLETL"
     gene            600..2048
                     /gene="hslU"
                     /locus_tag="PKO06_06110"
     CDS             600..2048
                     /gene="hslU"
                     /locus_tag="PKO06_06110"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNV69254.1"
                     /translation="MTETVEPGTIPQVICLPRGERTPPELVAELDKFIVGQHQAKRAV
                     AVALRNRFRRRQVGDALREDIKPRNILMIGPTGIGKTEIARRLAQIAAVPFIKVEATK
                     FTEVGYVGRDVDSMVRDLVEISIRMVRATRTDKVAPKARERAFERVLDILLPDPNRNR
                     HPLSGVERDSEKPGEMPIDRKDSDSMAHTREKFLARLKAGEFDTKMIEIEVTESATPV
                     VEVISQSGVEEMGFNMQGLQSMLGNMFPGRRRRRKVKVAEAREIFYHEELNQLIDQEE
                     VHREALRLAEESGIIFLDEIDKIASSYTHKTGPDVSREGVQRDLLPLVEGTTVTTKYG
                     MIKTDHVLFIAAGAFNISKPSDLIPELQGRFPVRVELEKLTAAEFERILTEPQNALLK
                     QYTELLRVDGVSLHFTPGAISRIAMIAQEVNTTQENIGARRLHTILERLLEDVSYDAP
                     SRISGEYEIDEAKVEKVLAPLLKDQDLARFIL"
     gene            2067..2489
                     /gene="flgB"
                     /locus_tag="PKO06_06115"
     CDS             2067..2489
                     /gene="flgB"
                     /locus_tag="PKO06_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNV69255.1"
                     /translation="MFCQLTETSTFNLLYKGLDVAAKRHQVISNNISNVNTPKFKRQI
                     VTFEDQMAKVFDGKVDLVGKRTEDRHIPIEEISYLDVKPTTITDRIHVMRNDKNNIDI
                     DVEMSDLAKNTMQYQIQATRLAAMFEGVNDVITRGGRV"
     gene            2489..2929
                     /gene="flgC"
                     /locus_tag="PKO06_06120"
     CDS             2489..2929
                     /gene="flgC"
                     /locus_tag="PKO06_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007953644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNV69256.1"
                     /translation="MNFFSAIDTSATGLTAERLRMDVISNNIANVNTTRTEAGGPFRR
                     QLCIFQARKPHGNWPHTERKNYSEPGKGVRVTAIMEDMAPFKLIYDPNHPDCDKDGYV
                     RMPNINVVHEMVDMITATRAYEANVTCINSAKDIINSALGIGRR"
     gene            2939..3241
                     /gene="fliE"
                     /locus_tag="PKO06_06125"
     CDS             2939..3241
                     /gene="fliE"
                     /locus_tag="PKO06_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNV69257.1"
                     /translation="MRVVESNPFSSILRNAQPQQKTNAQELWQGFADRLQGAIDEVDC
                     LEKHSQKMTQDAVLGRVENLHDVMIAAEKARTAMNLTLEVRAKAIESYKEIMRMQF"
     gene            3322..>4437
                     /gene="fliF"
                     /locus_tag="PKO06_06130"
     CDS             3322..>4437
                     /gene="fliF"
                     /locus_tag="PKO06_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNV69258.1"
                     /translation="MTEFLRQIWEPIAKLPRNQQLAIGLIIGLVFFGIIIASMWGGQQ
                     EYTVLFEEELKLEDASKVVAKLKELNIKYKLGKNSVDIQVPLADKSYILLQLAQEKTL
                     PQARPGWQKLIDDRSPFSGTTQQEFELNFVRGLQEELESGLVRLEPIENAKVYIVKPK
                     KEIFKEDQKEPTASVHLKLKPNADITKDQVRAIRSWVSSAVEGLKAENVRILDNNARD
                     LTRVLDEEEDMGLDKVKSVQVKFTQEMEKRLEKKLQSQLEEVFGSGKAVVRVSANLDF
                     DQKEAVSDVIIPPVEGASQGVEISSKTENEAYEGVDLVQDGEPGVNSNLPPGAPAYPG
                     TDNRTRNIYNRTAAIKNFDFTRSKEKFVKEQGNVRRLS"
BASE COUNT         1086 a         1347 c         1242 g          762 t
ORIGIN      
        1 gcacgtcagc cgcgaagggc tgaagaaggc ctacaaagga tcgcacccac gcgcatgacc
       61 cacgaacctc ttatgcacgg aaccacgatt ctcgccgttc ttcgcgacgg aaaactcgcc
      121 atggccggtg acggccaggt cacgctcggc cagaccattc tcaaggcccg tgcccacaag
      181 gtgcgtcgct tgcacggtgg caaggtactg ggtggttttg ccggccacac ggctgacgcc
      241 ttcacgctgt tcgaacggtt tgaagcgcag ctcgagaaag cctcggggaa cctcaagaag
      301 gccgcggtcg atctcgccaa agactggcgt ctcgatcgcg ccctgcgccg tctcgaagcc
      361 atgcttctgg tcggcaacca cgaccagatt ttactcatct cgggtcaggg cgacgtggtc
      421 gagcccgacg aaccgatcgc cgccatcgga tcgggcggca tgtatgcgct ggctgccgcg
      481 aaagccctgt ttcgacacac gacgctcgat gccgagacga tcacacggca ggccctgggc
      541 atcgccgccg agcagtgcgt ctataccaac gccaacctga gcctggagac cctgtaacga
      601 tgactgaaac cgtcgaaccc ggcacgattc cgcaggttat ctgcctgccc cggggtgagc
      661 gcaccccacc ggaactcgtc gcagaactcg acaaattcat cgtcggccag catcaggcca
      721 agcgtgccgt cgcggtcgcc ctgcgcaacc ggtttcgccg gcgacaggtc ggcgatgcgc
      781 tgcgcgaaga catcaaaccg cgcaatattc tgatgatcgg gcccaccggc atcgggaaaa
      841 ccgagatcgc ccgccggctc gctcagatcg ccgccgtgcc tttcatcaaa gttgaggcga
      901 ccaagtttac cgaggtcggt tatgtcggcc gagatgtcga ttcgatggta cgcgatctgg
      961 tcgagatcag cattcgcatg gtccgtgcca cgcgcaccga caaggtcgca cccaaagctc
     1021 gcgagcgggc cttcgaacgc gtcctcgaca tcctgcttcc cgacccgaac cgcaaccgtc
     1081 acccgttgag cggggttgaa agagactcag agaaacctgg ggaaatgccg atagatcgta
     1141 aggatagtga ttcgatggca cacacccggg aaaaattcct ggcacgtctc aaggcaggag
     1201 agttcgacac caagatgatc gagatcgagg ttaccgaaag cgctaccccc gtcgtggaag
     1261 tcatttccca gagcggggtt gaggaaatgg ggttcaacat gcagggtctg cagagcatgc
     1321 tgggcaacat gttccccggg cgccgccgcc gccgcaaggt aaaggtggcc gaggcccgcg
     1381 agatctttta tcacgaagaa ctcaaccagc tgatcgacca ggaagaagtc caccgcgaag
     1441 ctctgcgact cgccgaagaa agcggcatta tctttctcga cgagatcgac aaaatcgctt
     1501 cgagttacac gcacaagact ggccccgatg tctctcgcga aggcgtgcag cgtgacctgc
     1561 tacccctcgt cgaaggcacc accgtgacga cgaagtacgg catgatcaag accgatcatg
     1621 tgctgttcat cgccgccggc gccttcaaca tcagcaagcc gtctgacctg attcctgagc
     1681 tgcagggcag gtttcccgtg cgcgtcgagc tcgaaaagct gaccgcggcg gaattcgaac
     1741 gcattctgac cgaaccgcag aacgccctgc tcaagcagta taccgagctg ctgcgggtcg
     1801 acggtgtcag tctgcacttc accccgggcg ccatctcccg catcgcgatg atcgctcagg
     1861 aggtgaacac gacccaggaa aacatcggcg cccgacgatt gcacaccatt ctggaacggc
     1921 tgctcgaaga cgtcagctac gacgccccga gccgcatctc cggcgagtat gagatcgatg
     1981 aagcgaaggt cgagaaggtt ctcgccccgc tgctcaaaga tcaggatctc gcccggttca
     2041 ttctgtaaac ggagtcagga gaacacatgt tctgccagct cactgaaacc tcgaccttca
     2101 atctgctcta caagggcctc gatgtcgccg ccaagcgaca ccaggtcatc tcgaacaata
     2161 tctcgaacgt gaatacgccc aagttcaaac gccagatcgt cacgttcgaa gatcagatgg
     2221 ccaaggtgtt cgacggcaaa gtcgatctcg tcggcaagcg caccgaagat cgccacattc
     2281 ccatcgaaga gatctcgtat ctcgatgtca aaccgaccac gatcaccgac cgcattcacg
     2341 tcatgcgcaa tgacaagaac aacatcgata tcgacgtgga aatgagcgat ctggccaaga
     2401 acacgatgca gtatcagatc caggcgacac gcctggcggc catgttcgaa ggagtcaacg
     2461 acgtcatcac gcgaggaggt agagtctgat gaacttcttc agcgccattg acacctccgc
     2521 gaccggcttg accgcagaac gcctgcggat ggatgtcatt tcgaacaaca tcgcgaacgt
     2581 caacaccacg cgcaccgagg cgggcgggcc gttccgccgg caactgtgca tctttcaggc
     2641 ccgcaaaccg cacggcaact ggccgcacac cgagcgcaaa aattacagcg agcccggcaa
     2701 aggcgtgcgc gtcaccgcca tcatggaaga catggcgccg ttcaagctga tctacgaccc
     2761 gaatcatccc gactgcgaca aagacgggta tgtccgcatg cccaacatca acgtcgtgca
     2821 cgagatggtc gacatgatca cggccacccg cgcctacgaa gcaaacgtca cgtgtatcaa
     2881 ttccgccaag gacatcatca acagtgccct cggcatcgga cggaggtaat agaacgtcat
     2941 gagagtggtt gaatccaacc cgttctcttc cattctgcgg aacgcgcagc cgcagcagaa
     3001 gaccaatgcc caggaactct ggcagggctt cgccgaccgg ctgcagggag ccatcgacga
     3061 ggtcgactgc ctcgagaagc actcacagaa gatgacgcag gatgccgtgc tcggacgggt
     3121 ggaaaacctg catgacgtca tgatcgccgc cgaaaaggcc cgcacggcga tgaacctgac
     3181 gctcgaagtt cgcgccaagg cgatcgagtc ctataaagag atcatgcgaa tgcagttctg
     3241 atgttggttg tagccttggg caaaccagag aaaacagcct gaggtggttc gagtctgtgg
     3301 agtacagtag ggagtatagc ggtgacagaa ttcttgcgac aaatctggga accgatcgcg
     3361 aaactgccgc gcaatcagca gctcgcgatc gggttgatta tcgggctggt cttcttcggc
     3421 atcatcattg cctcgatgtg gggtggccag caggaataca cggttctgtt cgaagaagaa
     3481 ctgaaactgg aagacgccag caaagtcgtt gccaagctca aagagctgaa catcaaatac
     3541 aagctgggca aaaacagcgt cgacatccag gttccactcg ccgacaagtc gtacattctg
     3601 ctccaactcg ctcaggaaaa aaccctgccg caagcccgcc cgggctggca gaaactgatc
     3661 gacgaccgct ctcccttttc cggcaccacg caacaggaat tcgaactgaa cttcgtccgc
     3721 ggtctgcagg aagaactcga gtcgggtctg gtgcgactcg aaccgattga aaacgccaag
     3781 gtctacatcg ttaagcccaa gaaagagatc ttcaaggaag accagaagga accgaccgcc
     3841 agcgttcacc tcaaactcaa acccaatgcc gacatcacca aggatcaggt gcgggccatt
     3901 cgcagctggg tttcttcggc tgtcgaaggg ctgaaagccg agaatgtccg aattctcgac
     3961 aacaatgccc gagatctgac gcgcgttctc gatgaagaag aagacatggg cctcgacaag
     4021 gtcaagtcgg ttcaggtcaa attcacgcag gaaatggaaa agcggctcga gaagaagctg
     4081 cagagccagc tcgaagaagt gttcggctct ggcaaagccg tcgtgcgcgt gtcggccaat
     4141 ctcgacttcg accagaagga agccgtcagc gacgtgatca ttccgccggt cgaaggcgct
     4201 tcgcaaggcg tcgagatctc gtcgaagacc gagaatgagg cgtatgaagg cgtcgatctg
     4261 gtgcaggacg gagaaccagg cgtcaattca aacctgcccc ccggcgcgcc cgcgtatccc
     4321 ggcacggata accgcacccg caacatctac aatcgcaccg ccgccatcaa gaacttcgat
     4381 ttcacccgct cgaaagagaa gttcgtcaaa gagcagggga acgtgcgtcg tctttcc
//
LOCUS       DAORHD010000227         4419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21062_length_4419_cov_3.905133, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000227 DAORHD010000000
VERSION     DAORHD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21062_length_4419_cov_3.905133"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2257
                     /locus_tag="PKO06_06135"
     CDS             <1..2257
                     /locus_tag="PKO06_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69259.1"
                     /translation="FTLFEIDICALVDQALMVLLDGIGGGLRQQLDEFLPTGTNPQVG
                     YLRAFTTLHTLAHVLMNIIIAPNAQPPAYPIRKTLDDIPFPANPEAMWPAGALPAGVA
                     TNRNASIEKVGTMLWRAEASLTGSDGHVTTRMIEGRKDFRLADIEPPAPMYSLFVLNR
                     ENQRLAFNNLGGSLYVNNSVPGEPAGSATAEIPGLIRVNFANGRNGVPLDVRTSLMGN
                     PMIGLRSPLDENDGPIKQMFRGTDVLLVLDSASNMAMMGGETTCIADFEHCDRQPTTP
                     LQDTFGTRNVALIDPNEQDEKNALVQRQFDAIAVGDPEVRQRLQRRYDQFTQAYERPS
                     STISDAERRFQNWYSSHQQTFQYLSLLNFIPNVGALSVHPVRLLVALAQLALYGPPQV
                     NSWTATTFLARNDCFVGWEMPYFGTDWRPFTMPIPPWNLGATHLFGAGAMHPTLTREI
                     EGKVVKSYRQWKLCICGLQPLDALWIGGIRVPPVPFPVWYTGVIQNKYGYNFLPLQSF
                     DASANDIYTKVKEYDPEEPQNGVPNLYSSTQYARKASLFFDRWQDFRTDLETRLVQNA
                     SGQWVMPLPGITYITGDLGSSGEYFQPRVPAGLASAGQPISEVWVAGRGMLVCSGNIY
                     LNCSFRALPHSAGEAASATPPLFTLMARNGGLLLKNSVEGGQRFYRIDGSLYTDLGLM
                     VPDNCSLRILGNWVTNRFPRAAIRGTTAVDYLSSRVRSSLGSLHPDHGRLDPSRYYGT
                     LSPTWIAWQEK"
     gene            2259..2777
                     /locus_tag="PKO06_06140"
     CDS             2259..2777
                     /locus_tag="PKO06_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69260.1"
                     /translation="MGQIVKNHRTLFPGRGMSLVEVLIAVTILVLVIIGLSRVITGSD
                     AHTGRIGHVARATRLLQQVLEECQNRPLSQYLALFPERTATGSRELEPAFFPNTETAI
                     VAFRGEYQKSFQFFSRRVAARPVFNTLGQMREVWLEAEIRWTETRPEAGTASSPPRVL
                     RAGGLITNLEVQ"
     gene            2787..4295
                     /locus_tag="PKO06_06145"
     CDS             2787..4295
                     /locus_tag="PKO06_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69261.1"
                     /translation="MRRSRSALTLVEILVVTVLAVMVLSVAVRLWRQYSFEVARTATR
                     QLLQQQARQIMQTLAADVRSADASGISETGAAESGSTTVSQIRLARVFFQTGETTGSP
                     ETARAVRYEVAPPLLRRIGPLSAQSPVDCGKIIGNNIEECHLVVTTIGSATENSSGEP
                     TAVSPAASSAQGLEIILSLATLVPGSHFTVRHVERTTVFPRAAVSQIQHPGARTLASM
                     QTLDTASFQQAGDNTLINAFGGDGRLTADLVASLSDGDLAQLKSAQRANLDALDEMVT
                     NVNNAISRTKPSDDWSVAAWFKTTNLEAIRDLGDEIRAADDVETLEARKRDLDRFFDG
                     FGEEKLQEAFGTRGNASESSRDHALKLEVMRMRMRDREILISRQETTGAASASTDRTQ
                     FETMQATIAANATRQPGETQEDFETRRAKEQRFVELYQNCNIGTLVGGSQADGTDVAI
                     GDETYDYQALQYAQNCRDLMTSKMTALQAKQMTEENIRVIEAEEARRRTAGN"
BASE COUNT          906 a         1284 c         1318 g          911 t
ORIGIN      
        1 ctttaccctg tttgagatcg atatctgcgc tctggttgat caagctctga tggtgctgtt
       61 ggatgggatt ggcggtggcc tgcgacaaca gctcgacgag ttcctgccga cgggcacgaa
      121 tccgcaggtc ggctatcttc gcgcatttac cacgcttcac acgttggcgc acgtgctgat
      181 gaacatcatc atcgctccca acgctcaacc accggcttac ccgattcgca agacgctcga
      241 tgacatcccg ttcccagcca atcccgaagc catgtggccg gccggcgcct tgccggctgg
      301 agtggcaaca aaccgcaacg cgtcgatcga gaaggtcgga accatgctct ggcgggctga
      361 agcatcgttg accggctcgg acggtcatgt caccacccgc atgatcgagg gacgcaagga
      421 ctttcgactg gcggacattg agccaccagc gccgatgtac agcctgtttg tcctgaatcg
      481 tgagaatcag cggctggcgt tcaataatct tggtggttct ctctatgtga acaactcggt
      541 gcctggagaa cccgccggat cagcaacggc agagattccc ggtttgatcc gggtgaactt
      601 cgccaacggc cgcaacgggg tgccgctcga tgtgcgcact tccttgatgg gaaatccgat
      661 gatcggtctg cggtcaccgc tggacgaaaa cgacggaccc atcaaacaga tgtttcgtgg
      721 gacggatgtt ctgctggtac tcgacagcgc gtcaaacatg gccatgatgg gcggcgaaac
      781 cacctgcatc gccgatttcg aacattgcga tcgtcagccg accactccgc tccaggatac
      841 gtttggtacc cggaatgtcg cgttgattga tccgaatgag caggatgaga agaacgccct
      901 ggtacaaaga cagtttgacg ccatcgcggt cggtgacccc gaggtgcggc aacgtctgca
      961 gagacgatac gatcagttca cccaggcata cgagcggccc tcttcgacga tctcggatgc
     1021 ggaacgtcgg tttcaaaact ggtattcaag ccatcagcag acgttccagt acctgtcgct
     1081 cctgaacttc attcccaatg tcggcgctct cagtgtccac ccggttcgcc tgctcgtggc
     1141 gctggctcag ctggcgctct atggcccgcc gcaggtgaat tcctggaccg caacgacctt
     1201 tctggctcgc aatgactgtt ttgtcggctg ggagatgccg tatttcggta cagactggcg
     1261 ccccttcacc atgcccattc ccccgtggaa cctgggagcc acccatctgt tcggcgcggg
     1321 agccatgcac ccgaccctga ctcgggaaat cgagggcaag gtggtgaaga gctatcgcca
     1381 gtggaaactg tgtatctgcg gtctgcagcc gctcgatgcc ctctggatcg gcggtattcg
     1441 cgttcctccg gtaccattcc ctgtctggta caccggggtg atccagaaca aatacgggta
     1501 taactttctc cccctgcaat ccttcgatgc cagtgccaat gacatctaca cgaaagtgaa
     1561 agagtacgac ccggaagagc cgcagaatgg cgtcccaaac ctgtatagct caacgcaata
     1621 tgctcgcaag gcatcgctct tctttgaccg atggcaggat tttcggacgg atctcgaaac
     1681 gcgactggtt cagaatgcca gtgggcaatg ggtcatgccg ttgccgggaa tcacctatat
     1741 caccggagat cttggctcga gcggcgagta ctttcaaccg cgggttccgg ccggtctcgc
     1801 cagcgccgga cagccgatca gcgaagtgtg ggtggccggc cgcgggatgc tggtctgttc
     1861 agggaacatc tatctgaact gcagtttccg agcgttgccg cactcggcgg gcgaggcggc
     1921 gtcggccacc ccgccgctgt tcacgctgat ggcacgaaac ggcggtctgc tcctgaagaa
     1981 cagcgtggag ggtggacagc gcttctatcg tattgatggt tccctgtata ccgatctcgg
     2041 gctgatggtg cccgataact gttctctgcg cattctcggc aactgggtga ccaaccggtt
     2101 tccccgggcg gcgattcgcg gcacgacggc cgtggattac ctgagttcgc gcgtgcgctc
     2161 gagtctcggc tccctgcatc cggaccatgg tcgcctcgat ccgtcacgat actatggcac
     2221 gctgagcccg acctggattg catggcagga gaaatgacat gggacaaatc gtgaagaatc
     2281 atcgcacgct gtttcccggt cgcggcatgt cgctcgtgga agttctgata gccgtcacga
     2341 ttctcgtgct ggtcatcatc ggtttgagcc gggtcatcac cggctcggat gcccataccg
     2401 gccgtatcgg ccacgttgct cgcgccacga gactgctgca gcaggtgctc gaggaatgcc
     2461 agaaccgacc actgtcacag tatctggcat tgttccccga acgtaccgcg acggggtctc
     2521 gcgaacttga accggcattt ttccccaata ccgagacggc gattgttgct tttcgggggg
     2581 agtatcagaa gtcatttcag ttcttttccc gacgggttgc ggccaggccg gtcttcaata
     2641 cgctggggca gatgcgcgag gtctggctcg aggccgaaat ccgctggacg gaaactcgac
     2701 cggaggcagg aacggcttct tcgccgcctc gcgttcttcg agccgggggg ctgatcacca
     2761 atctcgaggt gcagtaggag tttgctatga gacgttcacg ttcagcgttg acgctggtcg
     2821 aaatcctggt cgtgacagtt ctggccgtca tggtgctcag tgtggccgtt cgcctgtggc
     2881 ggcagtattc gttcgaagtg gctcgcacgg cgactcgcca gctcctgcaa cagcaggcgc
     2941 gccagatcat gcagacgctg gcggccgacg tgcgctcggc ggatgcttcc ggcatcagcg
     3001 aaaccggcgc ggccgaatct ggttcgacga ccgtatcgca gatccggctg gcccgtgtct
     3061 tctttcaaac cggtgagact accggcagcc ctgaaacggc gcgggctgtg cgctatgaag
     3121 tggctccgcc tttgttgaga cgcatcggac cattgtcggc gcagagcccg gttgactgcg
     3181 gcaaaatcat tggcaacaac attgaagagt gccatcttgt ggtgacgaca attggctcgg
     3241 caaccgagaa ttcgtctggc gaaccaactg cggtttctcc tgcggcaagc tcggcgcagg
     3301 gtctcgaaat cattctgagc ctggcgaccc tggtgccggg gtctcatttc accgttcggc
     3361 atgttgaaag aaccacggtg tttccacgcg cggctgtatc tcagattcag cacccgggag
     3421 ctcggactct cgccagcatg cagacgctgg acaccgcttc atttcagcag gctggcgaca
     3481 acacgctgat caatgcgttc ggcggcgacg gccgactcac cgccgacctg gttgccagtt
     3541 tgagcgatgg cgatcttgcc cagctcaagt ctgcccagcg cgccaatctc gacgcccttg
     3601 acgagatggt gacgaatgtg aacaatgcca tttcccggac gaagccatcg gatgactggt
     3661 cagtggcggc ctggttcaag acgaccaatc tcgaagccat tcgggatctc ggtgatgaga
     3721 tcagagccgc tgatgacgtt gagaccctcg aggcgagaaa acgcgatctc gatcggtttt
     3781 ttgacgggtt cggcgaagaa aaactgcagg aagcgttcgg cacgcgcgga aacgcatctg
     3841 aatcgtcgcg tgatcacgcg ctcaagctcg aagtgatgcg catgagaatg cgagaccgcg
     3901 agattctcat cagccggcag gaaacgacgg gtgccgccag tgcgtcgacc gatcgaacgc
     3961 agtttgaaac catgcaggcg acgatcgccg ccaatgcgac gcgacaaccg ggcgaaaccc
     4021 aggaagactt cgagacccga agagcgaaag aacagcgatt cgtcgaattg taccagaact
     4081 gcaatatcgg cacacttgtc ggaggctcgc aagcggatgg aacggatgtc gcgattggcg
     4141 acgaaaccta tgattatcag gcgcttcaat atgcgcaaaa ctgtcgagac ctgatgacca
     4201 gcaaaatgac ggcgttgcag gcgaagcaga tgaccgaaga gaacattcgg gtcatcgaag
     4261 cggaagaagc cagacgtcgc accgctggaa actagagcgt cgcgtgcttc atcgcgttgc
     4321 cgctcgaaga ccggttatgg gtctggtgcc tctgcgacca ttcttcatgc cagaggcggc
     4381 gatgactttt tccgggtcag aatcacgagc gtcagatca
//
LOCUS       DAORHD010000228         4412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21118_length_4412_cov_3.959835, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000228 DAORHD010000000
VERSION     DAORHD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21118_length_4412_cov_3.959835"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PKO06_06150"
     CDS             <1..438
                     /locus_tag="PKO06_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV69262.1"
                     /translation="ANGVNRENGKRTAPAPLNMPFLQIFVAGAQGVWLYQPRDHSLSR
                     VSEKDLRSSLPKQPAYASASHLLLLVADLSKFAKPEIATESKLMWAHCNAGHIGQNVY
                     LAAAALDLGTCMVANVATESARTDLELRDDQFPLYLMPLGYVK"
     gene            454..1161
                     /locus_tag="PKO06_06155"
     CDS             454..1161
                     /locus_tag="PKO06_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69263.1"
                     /translation="MTELPPAPVFAPQQGSGVRTGSYSLFRPLRGGLLWILLCLCTFV
                     IPLESTWWLVCKITTDLFVMLLIAHLLFPGLSVLLRDGLKNEAMIQVALALIIWVTPM
                     VFNIGSIYWHTSINYAFLHDCSPQVNYLKRDKTDERMVLMIRHEMRQRWNALSAFRSN
                     LLRSLTPFFLLPENRFLDFELYLKQADRDEKEVLALFGNSVFCWVLLLVSCGAAGFLA
                     SVFPTPKLPPEAGSVVP"
     gene            complement(1174..1647)
                     /locus_tag="PKO06_06160"
     CDS             complement(1174..1647)
                     /locus_tag="PKO06_06160"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNV69264.1"
                     /translation="MDAKILNAFVGSLLDVFDRGAQMKLEKTKVSALPPQSRLKVEVA
                     SILGITGDIKGQMVMLTSEQIIMKCTSAILMGIEVTEFNEMAESGACEMLNMVAGDAS
                     QKIGELGYKFDISPPSVIHGNAEVTFPGSAPVFLIELKSDLGPVLVAITLEVVKK"
     gene            complement(1762..1995)
                     /locus_tag="PKO06_06165"
     CDS             complement(1762..1995)
                     /locus_tag="PKO06_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV69265.1"
                     /translation="MNVHVKIFGALQKPQGRDDFSHEIKDGATVEKLLLDLGYHPRHV
                     SAIVVELNGKQTRHTAKLAAGDELVLSLAIGGG"
     gene            complement(1992..2750)
                     /locus_tag="PKO06_06170"
     CDS             complement(1992..2750)
                     /locus_tag="PKO06_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HNV69266.1"
                     /translation="MVDERSGTDDLTRYQRHLSLDEIGMVGHETLRRARVAVVGVGGL
                     GSPVAAYLAVAGVGELRLIDDDTVELSNLNRQLLHATSDLGVTKTRSAARRLNDLNPE
                     CRLDLQPVRLQAGNADSLLAGCSLVVDCLDSFSSRRLLGEVCLRLKVPMIHGAVRGWE
                     GRLTTIVPGRSPCLACWLPPGADEQKPPVLGVVPGVIGALQATEAIRCLLRLEPLLTG
                     TLLLYDGRTMMFRRCALEHRSDCPSCGQGGGKPT"
     gene            complement(2740..4134)
                     /locus_tag="PKO06_06175"
     CDS             complement(2740..4134)
                     /locus_tag="PKO06_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69267.1"
                     /translation="MVRTCVRRVAWLMMCLSWLPLSLEAGNTLMVTGTWVSNLSVSVV
                     SAEINGKTEIICLPRLEFKEPIDRQALAQKMKEGVKRQEAIFHLYHDGIATPIRKNNL
                     LFSADVYLKEAKMTYIGLLREWGYGFKVIRKPPMDDPDYMQTIAAESMIGSLSATKKP
                     ATPKPASEGAAGIGQPGQKHPEGGSLSSSGGPLPSELLDIREKYSVMPAGVLHPSVRK
                     EKQELEAKLKELKSRPYKSETPRVDPVRWASGKMGLIESNGTISQAVVEGQRISLQAI
                     VPPKDWWKPTMRTALASQPCEFIFQRDNYGLEAIRDGKMMLADLYFSDLGQTWDEWLR
                     EQGSEPASMTSDPSWATGGVELKVRVAHGTWLELMAPSVAKIGFASPPAGLQSVELVR
                     LPPHDFPIKDYRTFRAAVDNVLKQRPVAVSLLYCLNGKPYTELGQKRALRVYLPQTKE
                     TVQLLMQKVATGGR"
     gene            4190..>4412
                     /locus_tag="PKO06_06180"
     CDS             4190..>4412
                     /locus_tag="PKO06_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69268.1"
                     /translation="MVAFPQAGDYAGEDNQDLERRIPTMTAVGKLTYQDLWQQEVGPL
                     VMMHAPLSGKGVAVLNRQGKISLFDERGQT"
BASE COUNT          932 a         1341 c         1129 g         1010 t
ORIGIN      
        1 gccaatgggg tcaatcgtga gaacgggaag cgcaccgccc ctgctccact caacatgccg
       61 tttctgcaga ttttcgttgc tggcgcgcag ggtgtctggc tctatcagcc tcgtgaccat
      121 tccttgtctc gcgtcagtga gaaagacctg cgatcatcac ttccgaaaca accggcctat
      181 gcttcggcgt ctcacctgct gcttctcgtt gccgatttgt ccaagtttgc caagccagaa
      241 attgcgactg aaagcaaact tatgtgggca cactgcaacg ccggacacat cggtcagaat
      301 gtctacctgg ccgcggcggc tcttgatctc ggaacctgca tggttgccaa cgtcgccacc
      361 gagtccgccc gcacggacct tgaactgcgt gacgatcagt ttccgctgta tctcatgcct
      421 ctgggatacg tgaagtaacc aggacgctga tgcgtgactg agcttccacc ggcccccgtc
      481 ttcgcgcccc agcaaggatc aggcgtccgt accgggagtt attccctgtt tcggcctctg
      541 cgcggcggat tgctgtggat tcttctgtgt ctttgcacct tcgtcattcc gctcgagagt
      601 acgtggtggc ttgtctgcaa aatcactacc gatctgttcg tcatgctgct gattgcgcat
      661 cttctttttc cgggattaag cgtgctcctg cgtgatggtc tgaaaaacga ggcgatgatt
      721 caggtcgccc tggctttgat catctgggtc acgcccatgg tattcaatat cggcagtatc
      781 tactggcaca cgtcgatcaa ctacgctttt ctgcatgact gctcgcctca ggtcaactat
      841 ctcaaaaggg acaagaccga tgagcgcatg gttctcatga ttcggcacga gatgcgacag
      901 aggtggaacg cgctctcagc ctttcgcagc aatctgctgc gttcgttgac tccgttcttt
      961 ctgttgcctg agaaccgttt tctggatttc gaactctatc tcaagcaggc ggatcgcgac
     1021 gagaaagagg ttcttgccct gtttgggaac agcgtcttct gctgggtgct tcttctggtc
     1081 agttgcggag ccgccggatt tctggcttcc gtgtttccga cgccgaaact cccaccggaa
     1141 gctggctccg tcgttccctg aacgaagaga tcgctatttt tttaccactt cgagagtgat
     1201 ggcgaccagt accggcccca ggtcactctt cagttcaatc aaaaacaccg gtgcgcttcc
     1261 cgggaaggtc acctcagcat ttccatgaat gaccgaggga ggcgaaatat cgaacttgta
     1321 cccaagctca ccgattttct gagaagcatc gccggcaacc atgttcaaca tctcacaggc
     1381 accgctctct gccatttcat taaactcggt gacttcgatg cccatcagaa tcgcactggt
     1441 acatttcatg atgatctgct cgctggtcag catgaccatc tgccccttga tatcgcccgt
     1501 gatgccaaga atcgacgcca cttccacctt cagacggctc tgcggcggca gcgccgaaac
     1561 cttggttttt tcaagtttca tctgtgcccc gcggtcaaaa acatccagca gggatccgac
     1621 aaacgcgttc agaatcttgg catccatggt tcatctcccg ttccgtagaa ttccggtgat
     1681 acaggcaagc gggaatcagt gcaacagtca gtctcgctca cagctttggt gccgtcagga
     1741 tactgcagct cagacgcgga atcaaccgcc accgatggca agtgacagga ccagttcgtc
     1801 ccccgccgcc agcttcgccg tatgtcgggt ctgtttgccg ttcagctcga cgacgatcgc
     1861 actgacatgc cgcggatgat acccgagatc gagcagcagc ttctcgacgg ttgctccgtc
     1921 cttgatctca tgggaaaaat catctctgcc ctgcggcttc tgcaacgctc cgaaaatctt
     1981 cacgtgtacg ttcatgtcgg ttttccccct ccttgcccgc atgacgggca atccgatcga
     2041 tgttcgagag cacatcggcg aaacatcatg gttcggccat catacaacag cagggttccg
     2101 gttaacaaag gctcgagcct cagcagacag cgaatcgcct cggtcgcctg cagagcgccg
     2161 atcaccccag gcacgacacc aagcaccggt ggtttctgct cgtcagctcc aggtggcagc
     2221 caacaggcca gacacggaga tcgtccaggg acgatggtgg tcaatcgccc ttcccacccg
     2281 cgaaccgcgc cgtgaatcat cggaactttc aggcgaaggc agacttctcc cagcaagcgt
     2341 cgtgacgaaa aggaatccag acagtccacg accagcgaac agcctgcaag aagcgagtcg
     2401 gcattcccgg cctgcaggcg aaccggctgc agatcgagac ggcattccgg gttcaagtca
     2461 ttgagacgac gggcggctga ccgggttttg gtcacgccca gatctgaggt ggcatgcagc
     2521 aactggcgat tcaggttcga caactcaacc gtgtcgtcat cgatgagacg cagttcgccg
     2581 acgccggcaa ccgccagata cgccgcaacc ggagacccca gaccgccaac tccgaccact
     2641 gccactcttg cccgccgcag tgtctcatga ccgaccatgc cgatctcatc gagactcagg
     2701 tggcgttgat atctggtcag atcatcggtt cccgatcgtt catcgaccac cagtcgccac
     2761 cttctgcatc aacagctgca cggtctcttt ggtttgcgga agatacacac gcagggctcg
     2821 tttctgtccc agttccgtat agggtttgcc gttcaggcag tataagagcg agaccgccac
     2881 cggcctttgc ttcaagacgt tgtccacggc ggcacggaaa gtccgatagt ccttgatggg
     2941 aaaatcgtgt ggcggcaatc gtaccagctc aacagactgc aaaccagccg gtggagatgc
     3001 gaatccaatt ttggcaactg aaggtgccat caattccaac catgtgccgt gcgcaactcg
     3061 gactttcaac tccacaccgc ctgtcgccca tgacggatca gaagtcatgc tggccggttc
     3121 acttccctgc tctcgcaacc actcatccca cgtctgacct agatcagaaa agtacaagtc
     3181 agccagcatc atcttgccgt cacgaattgc ctccaggcca taattgtctc gctgaaagat
     3241 gaattcacac ggctgagacg ccaacgcggt tcgcatcgtc ggcttccacc agtctttcgg
     3301 tggtacgatc gcctgcaggg aaattctctg gccttcaacc accgcctgag aaattgtccc
     3361 attcgactcg atcagcccca ttttccccga cgcccaccgc accgggtcga cgcgtggagt
     3421 ttcggatttg tagggtcgac tcttcagttc cttcagtttt gcctccagtt cctgcttttc
     3481 ctttcgcacc gatgggtgaa gcacaccggc aggcatgacc gagtatttct cccgaatgtc
     3541 gagcagctct gacggcagcg gtccacctga ggagctgaga ctgccaccct ccggatgctt
     3601 ctgtccaggt tgtccgatgc cggcagcgcc ttctgaagca ggttttggcg ttgccggttt
     3661 cttcgtcgca gacagcgatc caatcatgct ttcggcagca atcgtctgca tgtaatccgg
     3721 atcatccatc gggggcttgc gtatgacctt gaacccatat ccccactcgc gtaacaggcc
     3781 gatataggtc atcttcgcct ctttcagata cacatccgct gagaacagca gattattctt
     3841 gcgaataggc gtcgcaatac catcgtggta caagtgaaaa atcgcttcct gccgtttcac
     3901 gccttctttc attttctgag ccagggcctg gcggtcgatc ggttccttga actccagccg
     3961 cggcagacag ataatctccg tcttgccgtt gatttctgct gacaccacgg ataccgagag
     4021 attgctcacc caggtgccgg taaccatcaa cgtgtttccc gcttcgagcg agagcggaag
     4081 ccacgacaga cacatcatca accatgcaac ccgacgcaca caggttctga ccatcacagg
     4141 ttcctttcgc agttcgactc tggttacgag tatagcaaac cttggatcga tggttgcatt
     4201 cccacaggcg ggtgattatg ctggtgaaga caatcaggat ctggagcgaa gaatacccac
     4261 gatgaccgcg gtcggaaaac tcacctatca ggatctctgg cagcaggaag tcgggccgct
     4321 ggtgatgatg catgcgccac tcagcggcaa aggcgttgcc gtgctcaacc gccagggaaa
     4381 gatctccctg ttcgacgaac gtggtcagac gc
//
LOCUS       DAORHD010000229         4400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21229_length_4400_cov_4.037745, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000229 DAORHD010000000
VERSION     DAORHD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_4400_cov_4.037745"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKO06_06185"
     CDS             complement(<1..217)
                     /locus_tag="PKO06_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69269.1"
                     /translation="MNSLRGMLILVVCALVLVVGAGQARAESSPTAVVSEYLPIWPEG
                     ANVLRDEPVASEAAEIASGSSTLVVTNP"
     gene            complement(304..1065)
                     /locus_tag="PKO06_06190"
     CDS             complement(304..1065)
                     /locus_tag="PKO06_06190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69270.1"
                     /translation="MDIPRIFNIMESAHRIHNPFTPDKFATLGAALRLERGNRILDLG
                     SGSGEMLCTWARDHGITGTGVDMSPLFTEQAKKRAVELGVADRVTFVHGDAAGYVADE
                     KVHVAACVGATWIGGGVAGTFELLKRSLLPGGIILIGEPYWRQTPPTEEVAKNCLAHA
                     ISDFLTLPDLLASFGRLGCDVVEMVLATQDGWDRYEAAKWLTMRRWLATNPDDELAAE
                     IRDKLTTEPVRHATYTREYLGWGVFALMPREGAHP"
     gene            complement(1204..2022)
                     /locus_tag="PKO06_06195"
     CDS             complement(1204..2022)
                     /locus_tag="PKO06_06195"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNV69271.1"
                     /translation="MKIYVVCDLEGVAGVIAHRQQCQWDVAKAWYAPYLAQARRLATL
                     ELNALVEGALQGGATEIVAWDGHGSFPGGLDVELLHPECKLVMGAGDGGPAGLDESFA
                     GMFQLGLHAMAGTPRAVMAHSFQGNIVAYQVNDQCVGEIWMNCYTAGLFRVPCVFVSG
                     DRAAADEARALVPDVETAVVKEGLAETAGGLSVLPAISLSPQRAQAVIRDAAARAMTR
                     IGAVRPYRLEPPFRLRARFTAERYAEERAKQPNVKRLDTVTVEMERADYPWLLL"
     gene            complement(2230..2799)
                     /locus_tag="PKO06_06200"
     CDS             complement(2230..2799)
                     /locus_tag="PKO06_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNV69272.1"
                     /translation="MKTRTKLVAGIAVLVSVTLCSETALAGRKWSEKWRARVKKVFDT
                     VATGSAPVATKSGGVSASRGIVEELLALHNRERARAGAEALSLDERLCRAAQAHAQDM
                     ARNDRLDHTGSDGSNFWARIVKTGYDVTAGAENVAMGMNTSAEAVDCWMSSAGHRGNI
                     LNRKYRHAGFGFADSPNGDRYWCGVFGKE"
     gene            3014..3988
                     /locus_tag="PKO06_06205"
     CDS             3014..3988
                     /locus_tag="PKO06_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69273.1"
                     /translation="MKQPLVHTLFQIFVVCVAVFCLLVCTNTVSAQVAAESADKTLAS
                     GPNTVSERDASEIASECLNFQRYILQAVATLPQDLGPTELDPKDERWPDAPGKPSLDP
                     DSLFANKWLPGVMLKPGQGNGIATRPMLCDEHDDYQLTRDENGWLMNCERHGATPECA
                     ARLEKKQADESEAETKKCENFRRLAREALERNPAPTQEITHQLLWKGTPGAKLLPKDF
                     NWDAHPSSCEISAYHKEKGWDVRCNAHGFLQPVKNPEIETYSPPPPRGKLDFDWQEWL
                     IIVPVAIVVLYWRELLVAIAVIIGLIIAGVIFLGWYWRDKGKPTPPPQ"
     gene            4137..>4400
                     /locus_tag="PKO06_06210"
     CDS             4137..>4400
                     /locus_tag="PKO06_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69274.1"
                     /translation="MQGPDKGSPSIPLRLHWVGTGVVLLCLFVSIAVFLNASTSPPLY
                     YLLLWVSLPFLFSILPMHLRRDSATTRVSYILLVSVISILVSFV"
BASE COUNT          881 a         1395 c         1254 g          870 t
ORIGIN      
        1 ccgggttggt cacgacgagc gtcgacgaac ccgaggcgat ctcggcggcc tctgacgcga
       61 ccggctcatc acggagcacg ttcgctccct cgggccagat cggcagatac tctgaaacaa
      121 ccgccgtcgg cgacgactcg gcgcgtgcct gccctgcccc caccacgaga accagcgcgc
      181 agaccaccag tatcagcata ccacgcaacg aattcattcg ccgtgctgac aggtgagact
      241 gaccgatctt ttcgttttcc cgagacgcag gcaaagcctg taggcgctga aaccaaagac
      301 tggtcacggg tgcgcgcctt ctcggggcat cagggcgaag acgccccagc ccagatactc
      361 gcgtgtgtag gtggcatggc gcacgggctc ggtagtcagc ttgtctcgaa tttcagcggc
      421 gagttcatcg tccgggttgg tggcgagcca gcggcgcatg gtaagccatt tcgcagcctc
      481 atatctgtcc cagccgtcct gggtggccag aaccatttcg acgacatcac agccaagacg
      541 gccgaaagac gcgagcagat ccggcagggt aagaaagtcc gaaatcgcat gggccagaca
      601 gttcttcgcg acctcttcgg tcggcggtgt ctgccgccag tacggctcgc cgatgaggat
      661 gatgccgccg gggagcagac ttcgcttcag cagttcgaaa gtaccggcga ctccgccgcc
      721 gatccaggtg gctccgacgc aggccgccac atgaaccttt tcgtcggcga catatccggc
      781 ggcatcacca tgaacgaacg tgactcgatc agcaacaccc agttcgaccg cccgcttttt
      841 cgcctgctcg gtgaagagag ggctcatgtc gacaccggta ccggtgatac cgtgatcgcg
      901 cgcccaggtg cagagcatct cgcccgatcc gctgccgaga tcgagtattc tattccctcg
      961 ctccagtctc agcgctgcgc cgagcgtggc gaacttgtca ggggtaaacg ggttgtgaat
     1021 gcgatgcgcg ctttccatga tgttgaaaat gcgtggaatg tccaagttgg aaattctcct
     1081 ttttgtctga aacagcttca cacacggtat cactgattgg tgtcccggtg ccactcgaca
     1141 acccgggctg ttgatcatgg cgcttttcgc gcgtttgccg agagcagtgt ccaggttcag
     1201 acctcagaga agcagccagg gataatccgc tcgttccatc tcgaccgtca cggtatcgag
     1261 acgtttgacg ttcggctgct tcgcgcgctc ttcggcatat cgttctgccg tgaaccgcgc
     1321 tctgagtcga aagggcggtt cgagacgata cggacgcacc gcgccgattc gggtcatcgc
     1381 gcgggcggcg gcgtcccgaa tgacggcctg cgccctctgc ggagagagag agatcgcggg
     1441 cagcaccgac aagccccccg ccgtttccgc aagcccttcc ttgaccaccg cggtttccac
     1501 atccggcacg agggcccggg cttcgtcggc cgcggcccga tcgccgctga caaacacgca
     1561 cggcacccga aagagccccg ccgtgtagca gttcatccag atctcaccga cgcattgatc
     1621 gttgacctga tacgcgacga tgttgccctg aaacgaatga gccatcaccg cgcgcggcgt
     1681 gcccgccatc gcatgcagcc ccagctggaa catccccgcg aagctttcat cgagcccggc
     1741 cgggccgccg tcaccggcgc ccatgaccag tttgcattct gggtgcagaa gctcgacatc
     1801 gagccctccg ggaaagctgc catgaccgtc ccaggcgacg atctcggtgg caccgccctg
     1861 aagcgcgcct tcaaccagcg cattgagttc cagggtcgcc agtcgtcgtg cctgcgccag
     1921 atacggcgca taccaggcct tcgcgacatc ccactggcac tgctgccggt gagcgatcac
     1981 cccggccacc ccttccagat cgcacacgac atagatcttc acgcgcgcct cctcatatct
     2041 cggcacagaa tcttcataac ggtgcccaaa cttccaaggg aaacaggatt gaagcggcac
     2101 aatccgtcca ttaccggcaa cgtaagaccg cctcggaact caaatgcatg tcgtctccgc
     2161 gccatcgagc cggtggggtc gccaggagca gagacgcgag cgggatcgag cagaagcgag
     2221 ggagattcac tattcctttc cgaacactcc gcaccagtag cggtcaccgt ttggagaatc
     2281 ggcgaagccg aacccggcgt gccgatactt tcggttgagg atgttgccac gatgccccgc
     2341 gctgctcatc cagcagtcga cggcctcggc cgaggtgttc atgcccatcg ccacgttctc
     2401 ggcaccggcc gtgacgtcgt agcctgtctt cacgatgcgt gcccagaaat tcgatccgtc
     2461 cgagcccgta tggtccagtc ggtcgttccg ggccatgtcc tgggcatggg cctgcgccgc
     2521 gcggcacagc cgctcgtcca gggaaagagc ctcagccccg gcccgagccc gttcgcggtt
     2581 atgcagagcc agcagttctt cgacgatacc ccgtgacgcc gacaccccgc cggatttggt
     2641 cgcaacgggc gcagagccgg tcgcaacggt gtcgaagact ttcttcaccc gggctctcca
     2701 cttttccgac cactttcttc cggccagcgc cgtctcggaa catagcgtaa cactgaccag
     2761 aaccgcaata cctgctacca gcttcgtccg cgttttcatg atcattctgt ctcctctgcc
     2821 tttgtcgtcg ccagcattct gccccaggaa cggtgacccg cccttcgacc gattcctctc
     2881 ctgagcatca agaaaccagg taccagcctg ccttgtcggt tcttcagacc caccggcgca
     2941 gaaaatcgac ggttgacttg tgctatcatc gcccgagaga cgagagtcgt caactgcagt
     3001 gaggacccat tcgatgaaac agcctttggt acatactctt ttccaaatct tcgttgtctg
     3061 cgtggcggtg ttctgtctgc tcgtttgtac caacacggtt tcggcgcagg tcgctgcaga
     3121 aagcgctgac aagactctgg caagcggtcc taacacggtg tctgaacgtg atgcgagcga
     3181 gattgccagc gagtgcctga actttcagcg gtacattctg caggccgtcg caaccctgcc
     3241 ccaagatctc ggtccgacag aactggaccc caaagacgag cgatggcctg atgcgcccgg
     3301 aaaaccgagc ctcgacccgg attctctgtt cgcgaacaag tggctgcctg gcgtgatgct
     3361 caaacctggt cagggaaacg gcattgcgac ccgaccgatg ctgtgcgatg agcatgacga
     3421 ctaccagctc acgcgagacg agaacggatg gctcatgaac tgcgagcgtc atggagcgac
     3481 gccggaatgt gccgcgcgac tcgagaagaa gcaggcggac gaatcggaag ccgaaacgaa
     3541 gaagtgcgag aatttcagac gactggcgcg cgaggcactg gaacggaatc ccgcgccgac
     3601 tcaggagatc acgcatcaac tactctggaa gggaactcct ggtgccaagc ttcttccgaa
     3661 agacttcaac tgggacgctc acccatccag ctgcgaaatc tcggcctatc ataaggaaaa
     3721 aggctgggat gtccgctgta acgctcacgg gttcctgcaa cccgtgaaga accccgagat
     3781 tgaaacatac tcgcctccgc ccccgcgtgg aaagctggat ttcgactggc aggagtggtt
     3841 gatcatcgtc cctgtcgcca tcgtagtgct gtactggcga gagcttctcg tggcgatcgc
     3901 cgtcatcatc ggcctgatca tcgccggagt catttttctg ggctggtact ggagggacaa
     3961 gggcaagcca accccgcccc cacaataggg cggagcctga acgcagaccg tgccatcgat
     4021 ctcgacctgt tcatccacga acactgacag cagcgccgcg acccggaaat gggttgatct
     4081 aacatggaca tttttggaga atggatcaca tgggttcaaa tggggggggg taagtgatgc
     4141 agggtcccga taagggttcg ccgtccattc cgttgcgtct gcactgggtt ggcacagggg
     4201 tcgtgcttct ctgcctgttc gtgtccattg cagtgttcct caacgccagt acgagtcctc
     4261 ctctctacta tctgctgttg tgggtgtcgc tgccttttct gttctcgatt ctcccgatgc
     4321 acctccgaag agacagtgcc acaactcgtg tttcctatat tctgttggta agtgtcattt
     4381 ccatcttggt gagctttgtg
//
LOCUS       DAORHD010000230         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21303_length_4390_cov_3.232987, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000230 DAORHD010000000
VERSION     DAORHD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21303_length_4390_cov_3.232987"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            520..1641
                     /locus_tag="PKO06_06215"
     CDS             520..1641
                     /locus_tag="PKO06_06215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69275.1"
                     /translation="MKNLAIFSVSAGAGHVRAAMAIKATAERDFPGLKAEHVDIMDLV
                     PDLFRKLYADSYLKLVENHPTLWGYLYKKADREKVDSLLNKIRRAIERLNTRGFEAKL
                     AELKPDYVICTHFLPAELLSRLRRDGNFAAPVWVGVTDFDVHAMWIHQHMTGYFAAGD
                     EIAYRLADRGLERERIHVTGIPIMPAFSQKLDRTTCAKELGIDPNKFTAIMMSGGAGV
                     GGIEVLAERLLKLPGDFQIIALAGKNQELLEKLQGLAADHPKKIYPQGFTTTIERMMT
                     ASDVAITKPGGLTTSECLAMGLPMIVVSPIPGQEERNADYLLENGAALKAYDGAALEF
                     RMKRLLEDQGQLRQLRENARRIGRPEAAKRVLELVTREG"
     gene            1659..2138
                     /locus_tag="PKO06_06220"
     CDS             1659..2138
                     /locus_tag="PKO06_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69276.1"
                     /translation="MSEHWHPWLLLLWVTLLAFFFAKVEIQIEGANGWAANLPTWKIE
                     KHWLLDIFWGGRPMTGYHAWVFSFMALVFHLPIFVAGAWNWRLEARILALLMAFWIIE
                     DFLWFLINPAFGWSKFQPAFVPWHKNWYLRVPVDYLVFLGVGSVLYWLSFRGKSGKG"
     gene            2193..3923
                     /locus_tag="PKO06_06225"
     CDS             2193..3923
                     /locus_tag="PKO06_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69277.1"
                     /translation="MDELIRKDEKACGEQSVALSPFFRSGFELFCLWTIVTLFVLAHW
                     LCEAWGWAIGSLPRAQVNAGVFAFANLCAALLCWQGLRWIRLQGLVRQTAMRVSTAIW
                     FAATSNVLSFIMWLTLQTVNGPYRLVPFVLFLFSILFGIWGMFKLARLAHVSLGVKTF
                     HYYIGTLGTLLCMSYLLSPDLYSSEVMKQAHRVSILFHVVFTILISIIAAVSLHNCLD
                     SSGRLRHPAQLISAGTILLCFGCSIYASVQVRCRLDELQAWASPAFVILALSYVTIGL
                     GVFRMGSRVVESFAVQTESLPATELLQDFFGQDLGLRLYDELLGRIRTSEDALRRVTA
                     EKVRAEEANLAKSRFLVMMSHELRTPLTMLIGYANMLAHPDEFGEQVPPATQLGERIL
                     TSSHHLKKLIEGILDFSAIEQGKIVVRAEEFPASELIDFARSFGESQAASRNIDFRLV
                     VEGCAETISSDPQMLRQILVNLLNNAFKFTRQGSVTLKLEFQSGRLGFAVTDTGIGIA
                     SEHLERIFMPFYQVSQGRNRKFGGVGLGLTIVQRQIEALGGTIRVESDEGQGSTFAGE
                     VPLRSMANAA"
     gene            3988..>4390
                     /locus_tag="PKO06_06230"
     CDS             3988..>4390
                     /locus_tag="PKO06_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69278.1"
                     /translation="MTEIIRHAREVGLDFLCPTDHGTLAARTLAGWHEGLLLIPGMEV
                     NDLGHHVLAFGIEREVPANETCPQAVVDGVRAQGGTCFAAHPHDRTCRLMPGFRGCPW
                     TDWSVSGYSGIEIWNYMSTWKGEANDFASALK"
BASE COUNT          844 a         1197 c         1356 g          993 t
ORIGIN      
        1 gtcgtcttcg atctcgacgg cacgctgctg aacacgctcg gcgacctgac cgagtgcctc
       61 attgatgcag tcggtcaggt cgccgagcgt gttcagcagc gtgccgtcga gatcgaagac
      121 gacggcttga ggcgggcgag ggaagctgga catggccatg tgctccgtgc ggcgagatac
      181 gtatgcagac tcttccgaag aggcagggta tcacagtcgc ggaacaccgg agaggaatcc
      241 atacagggcc acagggcaac ggctcacggg aactttttca tcgcgtgacg gaccttgttt
      301 cccgaccacg tcgtaggcgt ccggtgcatg cgcgggggct gacgaaaatg agcaacaggt
      361 tcgtcactca ctaaaccgaa atctgagctc gccacgatat gtacctgttt cggtatcgct
      421 tttctgtagg gcggttgtgt ggtaaacctg ttatgagagc cggcgttcga aggcgttgcc
      481 gggaacggat gggatcacat tgatggaagg atgtgcagta tgaaaaatct ggcgatattc
      541 agtgtttcgg caggggcagg gcacgtacgg gcggcgatgg cgatcaaggc gacggcggaa
      601 cgggattttc ccggtctgaa ggctgagcat gtcgacatca tggatctcgt acccgatctg
      661 tttcgtaagc tgtatgccga ctcgtatctg aagctggtcg aaaaccatcc gaccctgtgg
      721 ggctacctgt acaaaaaggc tgatcgcgag aaggtcgatt cgctgctcaa caagattcgg
      781 cgggcgatcg agcgtctcaa cacccgcgga ttcgaagcga agctggccga actgaaacca
      841 gactacgtta tctgcacgca ctttctgcca gcagaactgc tgtcgcggct gcggcgagac
      901 ggaaactttg cggcgccggt gtgggtcggt gtcaccgact tcgatgtgca cgcgatgtgg
      961 atacaccagc acatgaccgg ctacttcgcg gcgggtgacg aaattgccta tcgactggcc
     1021 gaccgcggtc tcgaacgcga gcgcattcat gtaacgggaa ttccgatcat gcccgcattt
     1081 tcccaaaaac tggatcggac aacctgcgcg aaagaactcg gaatcgatcc gaacaagttc
     1141 acggcgatca tgatgtcggg cggtgcgggc gtgggcggca tcgaggtgct ggccgagcgc
     1201 ttgttgaagc tgcccggtga ctttcagatc attgcgctgg ccggcaagaa ccaggaactg
     1261 ctggagaagc tgcaggggtt ggcggcagat catccgaaga aaatctatcc gcagggcttc
     1321 acgacgacca ttgaacgcat gatgaccgcg agcgatgtcg ccatcaccaa accgggtggg
     1381 ttgaccacct cggagtgctt ggccatgggg ttgccgatga tcgtggtttc gccgattccg
     1441 gggcaggaag aacgcaacgc cgactatctg ctcgagaacg gtgccgccct caaggcatat
     1501 gacggcgcag cgctcgagtt ccgcatgaaa cggctgcttg aagatcaggg gcagctgcga
     1561 cagctgcgcg agaatgcccg gcgcatcggc cgccctgagg cggcaaagcg tgtgcttgag
     1621 ctggtgactc gcgaaggctg attcacatgg aatggaaaat gtccgaacat tggcacccgt
     1681 ggttgctgct gctgtgggtc acgctgctgg cgttcttctt cgccaaagtc gagattcaga
     1741 tcgagggtgc gaacggctgg gccgcgaacc tgccgacctg gaagatcgaa aaacactggc
     1801 tgctcgacat cttctggggc ggtcgcccca tgaccgggta tcacgcctgg gtcttctcat
     1861 tcatggcgct cgtcttccat ctgcccatct tcgtggccgg cgcctggaac tggcgtctgg
     1921 aagcccggat tctcgctctg ctgatggcct tctggatcat cgaggacttt ctctggtttc
     1981 tgatcaatcc ggcgttcggc tggagcaagt ttcagcccgc ctttgtaccc tggcacaaga
     2041 actggtatct acgcgtaccg gtcgactatc tcgttttcct tggcgtgggc agtgtgttgt
     2101 actggctgtc ctttcgggga aaaagtggca aaggctgaat gacgaacggc gttgatagtc
     2161 gctcgtctcg cccgcagagg aactgacgac gaatggatga actgattcgg aaagacgaaa
     2221 aggcttgcgg tgaacaatcc gtagccttgt cgcccttctt ccgcagcggc ttcgagttgt
     2281 tttgtctctg gacgatcgtc actctgttcg tgctggcgca ttggctctgc gaggcgtggg
     2341 ggtgggcgat cgggtctctg ccgcgagcgc aggtgaatgc cggcgtcttt gcctttgcca
     2401 acctctgcgc ggccctgctg tgctggcagg gtctgcgctg gatcagactt cagggactgg
     2461 tccggcagac ggccatgcga gtctcgaccg cgatctggtt tgcggccacc tcgaatgtgc
     2521 tgagttttat catgtggctg acgctgcaga ccgtcaacgg tccttatcgg cttgtcccat
     2581 ttgttctgtt tctcttttcc attctgttcg gcatctgggg tatgttcaaa ttagcccgac
     2641 tggcacatgt gtcgctgggc gtaaagacgt tccattacta tatcggaacc ctcggaacgc
     2701 tgctttgcat gtcgtatctg ctttcgcctg acttgtactc cagtgaggtg atgaagcagg
     2761 cgcaccgcgt gtcgatcctg ttccatgtcg ttttcaccat tctgatttcg atcatcgccg
     2821 cggtatccct gcacaactgt cttgattcga gcgggcgttt gcgtcatccc gcacagttga
     2881 tctccgcagg aaccattctg ctgtgctttg gctgcagtat ctacgcgtcg gttcaggtgc
     2941 gctgccgcct cgatgagctg caggcgtggg ccagcccggc attcgtcatt ctcgccctct
     3001 cctacgtcac catcggtctt ggtgtgtttc gtatgggcag tcgggtcgtc gagtcgttcg
     3061 ccgtgcagac cgagagtctt ccggctacgg aactgctgca ggattttttc ggacaagatc
     3121 ttggactgcg gctctatgac gagttgcttg ggcgcattcg cacctcggaa gacgccttgc
     3181 gacgcgtcac ggccgagaag gtgcgtgctg aagaggccaa cctggccaag tcacgctttc
     3241 tggtcatgat gagtcatgaa ctgcgcacgc ctctgaccat gctgattgga tatgccaaca
     3301 tgctcgcgca cccggacgag ttcggtgaac aggtgccacc ggcgacgcag cttggcgaac
     3361 gaattctgac cagctcgcat catttgaaga agttgatcga aggcattctg gatttttcgg
     3421 ccatcgaaca gggaaagatc gtggtgcgtg ccgaagagtt tccggcgagt gaactgatcg
     3481 attttgcacg ctcgttcgga gagtcgcagg cggcgtcgcg aaacattgat tttcggctgg
     3541 ttgtcgaagg ttgtgccgag accatctctt ccgacccgca gatgctacgc cagattctgg
     3601 tgaacctgct gaacaacgcc ttcaaattca ctcgccaggg atccgtcact ctgaaactgg
     3661 agtttcaaag cggtcgattg ggttttgccg tcaccgacac gggtatcggc attgcgtctg
     3721 aacatctgga acgcattttc atgcctttct atcaggtgag ccagggtcgc aatcgcaaat
     3781 tcggcggagt cggactcgga ctgacgatcg tgcagcgaca gatcgaagct ctcggcggaa
     3841 ccatcagagt tgagagtgac gaggggcagg gaagcacgtt cgctggtgag gttccgttgc
     3901 gttcgatggc caacgccgcg tagccccatg cgcagattac gcggtcatat tcatctgcat
     3961 tcggtctggt cagatgggac gcttcccatg accgagatca tccgccacgc cagagaagtg
     4021 gggcttgatt tcctttgccc gaccgatcat ggcactctgg cagctcggac gcttgccggg
     4081 tggcacgaag gcttgctgtt gattcctggc atggaggtca acgatctagg ccaccacgtt
     4141 ctcgcgttcg gcatcgagcg tgaagttccg gcgaacgaga cctgcccgca ggcggtcgtc
     4201 gatggcgtgc gcgcacaggg cggcacctgt ttcgcggctc atccgcacga tcgcacgtgc
     4261 cggttgatgc ccgggtttcg cggctgcccc tggaccgact ggagcgtgag tggttactcg
     4321 ggtatcgaga tctggaacta catgtcgacc tggaaaggcg aagcgaatga cttcgccagt
     4381 gccctcaaac
//
LOCUS       DAORHD010000231         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21374_length_4379_cov_4.398936, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000231 DAORHD010000000
VERSION     DAORHD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21374_length_4379_cov_4.398936"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1921
                     /locus_tag="PKO06_06235"
     CDS             275..1921
                     /locus_tag="PKO06_06235"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV69279.1"
                     /translation="MTPIVRSPQSVPPTPAPQPATFASFNLHPDLMRGIEQLGFETPT
                     EVQRNSIPVALSGRDLLACAMTGSGKTAAFLLPIMHGILTRRQAQKPEGFGHRSVTRA
                     LILTPTRELAAQIHEHMIELARFTTLRGAAVFGGVGMEPQTNAFRTGVDIIIATPGRL
                     LDHFTYPHGKLPHLEYLVLDEADRMLDMGFLPDIRRVLAQLPQTPRQTLFYSATMPEP
                     IVTLSQDMLRNPVAINIRNTTTPATGIKHAAYTVPENLKQHLLVEVFKHEAIRSAIVF
                     TRTKHRADRLAAYLNQNGIACLAIHSNRSQTRRTEALGSFKNGQCPVLVATDIAARGI
                     DVEALSHVVNFDVPHLAEDYVHRVGRTARASLKGTAITLVAPHEEIDFCMIEKKISKK
                     IQRRQVEGFNYRAPGEPLEIPLGQRIAAIRARKADERARSAAKARAKAEREALSAQQA
                     KRTQRSSLSTLSTRTSQVSATREQPTRRQTQGGSHHPSTRSAASDRSIPIWVDSTDAQ
                     NPRQQPSSSPRSSQPRDTNRGSSQEGRPANRGGHHHRRRS"
     gene            2365..3837
                     /locus_tag="PKO06_06240"
     CDS             2365..3837
                     /locus_tag="PKO06_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69280.1"
                     /translation="MTLSSDRRKFACDPNRAIIGLCFCKSRELSRISRPMSAEVCRRC
                     GAALPLKTDACPLCGVVDPRSSDLSYGERQFSQNNPEIPSEFSEYTSMIEANQSLVSN
                     LGEGVRHLLNNPDIAPLIWGAVASAILGIGAWVFVPAIWVSFFLFFPALLFLAVQFAL
                     MMRAVFFVHRYKRLRRTRSLAPYSVHFRMERQIRELLGGLHTLVRSVLDRDWRSSGTE
                     LNSSAESFMTAARVIVDRLRACATPSLEMTRIIWQNNVYAILASEMNPQEKAAAIGAK
                     CREGEALLLRYRWLITLPALLAEMEQHIQTSRQVGGSDPIKTSEVLDRYHLQPFGPME
                     EGVRETFDRTPADLLFTGRQYWIHRLPPMALEEDESYGSSTAAKAFFKSLKLVRPMRH
                     SLEEQMALNTAAMALSTVSPLEGGIPASAEARAISREGQLSHLVNIPEFKPDAGRLNT
                     EVDRLVAEARVAMGTDRMAERTDDTPESSEVPSPRQRLRE"
     gene            4008..>4379
                     /locus_tag="PKO06_06245"
     CDS             4008..>4379
                     /locus_tag="PKO06_06245"
                     /inference="COORDINATES: protein motif:HMM:NF039244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HNV69281.1"
                     /translation="MTRFRLALLLALFAVLPSIALVQAAGDPYSTSANAISRVPAAGT
                     AGTQTQSGTKPSTWGFGTQYKVEGLLTVHEEAITLSTADGRSFVLIMSGREAAKYHDR
                     QVVIEGSTHQADDLACLKVKKI"
BASE COUNT          903 a         1396 c         1182 g          898 t
ORIGIN      
        1 tcacggttct caaggtgcaa tcgtacaagt tttatcttgc atttaccctt atgaacaaat
       61 gttttccgaa tagtcgaaat aaagaagttg ccagcgggtc tgattcctga taagataaga
      121 gtgcaatctt gaatcagctt tttgtctgat cggatcgcac aacacaccgg gttgatttcc
      181 aaccccctgc ggcggcctct ccggcagatc ctccgttgtt ccctgtgccg ggtcattcgg
      241 caaagcatca accgcttccc gagaaagatt cattatgaca ccaattgtcc gttcacccca
      301 atcagtcccc cccacacccg ccccgcaacc tgcgacgttt gcctcgttca acctgcatcc
      361 agacctgatg cgcgggatcg agcaactcgg cttcgaaacg ccaaccgaag tgcagcgcaa
      421 ctcgattccg gtcgctctga gcggccgcga tctgctggcc tgcgctatga cgggaagcgg
      481 gaagactgcc gcattcctcc tgcccatcat gcatggcatt cttacccgcc gtcaggctca
      541 gaagccggaa ggattcggtc accgcagcgt cacccgcgcg ttgattctga ccccgactcg
      601 tgagctggcc gcgcagattc acgaacacat gatcgaactg gctcgtttta ccacgctgcg
      661 cggcgccgcc gtcttcggcg gagtcggcat ggaaccgcag acgaacgctt ttcgcaccgg
      721 tgtcgatatc atcatcgcca cacctgggcg cttgctcgat catttcacct acccgcacgg
      781 caagctgccg cacctcgaat atctcgtgct cgacgaggca gatcgcatgc ttgacatggg
      841 ctttctgccc gacattcgcc gcgtcctggc tcagttgccg caaactccgc gacagacgct
      901 gttctactcg gccacgatgc ctgaacccat tgtgaccctg tcgcaggaca tgctgcgaaa
      961 tccggtcgcg atcaacattc gcaacaccac gacgccggcc acggggatca aacatgcagc
     1021 atatacggtt cctgagaatc tgaaacagca ccttctggtc gaagtcttca agcacgaagc
     1081 cattcgcagc gccatcgtct ttacccgcac caagcaccgg gccgaccggt tggcggcgta
     1141 tctcaatcag aacggcatcg cctgtctggc gatccacagc aaccgcagtc agacgcgtcg
     1201 taccgaggct ctgggctcgt tcaaaaacgg gcagtgtccg gttctcgtgg cgacagacat
     1261 cgctgctcgc ggcatcgatg tcgaggcact cagccacgtg gtcaattttg atgtccctca
     1321 cctggccgaa gattacgttc atcgcgtcgg tcgcacggcg cgcgccagcc tgaaagggac
     1381 cgcgattacc ctggtggcac cacatgagga gatcgacttc tgcatgatcg agaagaagat
     1441 ttcgaagaag attcagcgcc gacaggtcga ggggttcaat tatcgagcac cgggcgagcc
     1501 gcttgaaatt cctctcggcc agcgcattgc cgccattcga gcccgcaaag ccgatgagcg
     1561 agcccgttca gccgcgaagg cccgtgccaa agctgaacgc gaggcactca gcgcacagca
     1621 ggccaaacgg acccagcggt catctctgtc cactctttcc acacgcactt cccaggtgag
     1681 cgcaacccgc gaacagccaa ctcgccgtca gacgcagggt ggcagccacc atccgtcgac
     1741 gcggtcggcg gcttccgatc gctccattcc tatctgggtc gactcgacag acgcgcagaa
     1801 cccgcggcag caaccctcgt catcaccacg ctcgtcccaa ccgcgtgaca cgaacagagg
     1861 gtcatctcag gaagggcggc ccgcgaaccg aggtgggcac caccaccgcc gccgctcgta
     1921 agtaagagcc tcgggtgttg cgcatcttca tcgcctgagc cgcacgatcg atcgcacgca
     1981 accaacggtc aggagagcca ctggctcttc tgaccgttgc tttttcccgt gcttcgagaa
     2041 acaccgactt cacccgcagc gacgcaaacg gccacgatct gcgcgtgaag cggcacctgg
     2101 gcagacctgt catctgacca gggtcatttc attccccgac ttacgtgttt tccgttcaca
     2161 tcgctcaacg attcaccgtt acggcaggga gtgcgctcgt cgtctcgaga ccgaggctga
     2221 aacctcgtcg acttcgcatc cctgcgaaga cgacgaggcc gaatcgcctc ctgcgatccg
     2281 gcagcctgtt ctcggactcc gcccgcctct tgccgaacgg ctcacaagtt tcgctcttgc
     2341 gaacggaaaa tgggcgagaa ttaaatgacc ctgtcgtctg accgccgcaa gtttgcgtgt
     2401 gacccgaacc gtgctatcat cgggctatgc ttctgcaaat ctcgcgaact ctcgcgcatc
     2461 agccgaccca tgagcgctga agtctgtcgc cggtgcggag cggcactgcc gctgaaaact
     2521 gatgcgtgcc cgttgtgcgg ggtggtcgac ccgcgctcga gcgatctgtc gtatggagag
     2581 cggcagtttt cgcagaataa tcccgagatt ccgagcgaat tcagcgagta cacctccatg
     2641 atcgaggcca atcagtcgct ggtctcgaac ctgggtgagg gcgtcaggca cctgctgaac
     2701 aacccggata tcgctccgct catctggggt gcggtcgctt cggccattct tggcatcggt
     2761 gcctgggttt tcgtccccgc gatctgggtt tcgttttttc tctttttccc cgcccttttg
     2821 tttcttgccg tgcagtttgc gctcatgatg agggccgtgt ttttcgttca ccggtacaaa
     2881 cgattgcgac gcacccggtc gctcgctccc tattccgtgc actttcgcat ggaacgacag
     2941 attcgagaac tgctcggtgg cttgcacact ctggtgcgat cggtcttgga ccgcgattgg
     3001 cgaagcagtg gcacggaact caacagttca gccgaaagct tcatgaccgc ggcgcgagtc
     3061 atcgttgatc gcttgcgcgc atgtgccacc ccatcactgg aaatgacgcg aatcatctgg
     3121 cagaacaacg tgtacgccat ccttgcctcc gagatgaatc cacaagaaaa agcggccgca
     3181 atcggcgcaa agtgccggga aggtgaagcc ctgttgctgc gctaccgctg gctgatcacg
     3241 ttgccagcgc tgctcgcgga gatggagcaa catattcaaa ccagccgaca ggtcggtggc
     3301 agtgacccca tcaagacatc agaagtgctc gaccggtatc atctgcagcc attcgggccg
     3361 atggaagaag gagtgcgcga aaccttcgac cgcacgcccg ccgatctgtt gttcaccggg
     3421 cgccagtact ggattcaccg gttaccaccc atggcgcttg aagaagatga atcttatggt
     3481 tcaagcactg cggcgaaagc ctttttcaag agtctcaaac tcgtacgacc gatgcggcat
     3541 agtctcgaag agcagatggc gctcaatacc gcagccatgg ctctttcgac tgtatcgcct
     3601 cttgagggcg gcataccggc gagtgccgag gcacgcgcca tcagtcgcga gggccaactc
     3661 tctcacctgg tcaatattcc cgagttcaag cccgatgccg gccgtttgaa caccgaagtc
     3721 gaccgcctgg tggcggaagc gcgggtcgcg atgggcaccg atcgcatggc cgagcgcaca
     3781 gacgacacgc cagaatcctc tgaagttccc tctccgcgac agcgtctccg cgagtagcct
     3841 gacgaccgcc agatcgcacc caggtgtatc ccggcgccac tttgcatcaa ctcaagcacc
     3901 ccttctgctc aggacattct gccgcatcct tcttccggga atgaggttga tcgggtatac
     3961 tgtacaaata cgaatcgatt tccgtctcgg cgctgaagga ggaaggtatg actcgttttc
     4021 gtctcgccct tctgctggca ctgttcgcag ttcttccgtc cattgccctg gttcaggctg
     4081 caggcgaccc ctattccacc agtgcaaacg ccatttcgcg tgttccggcg gctggaaccg
     4141 cgggcaccca gacccagtca ggcacaaaac cttcgacctg gggattcggc actcaataca
     4201 aagttgaagg cctgctgacc gttcacgaag aagccattac tttgagcacc gccgacggtc
     4261 gctcctttgt tctgattatg tccggccgcg aggcagcgaa atatcatgat cgtcaggtgg
     4321 tcatcgaagg ctcgacacat caggccgacg acctggcgtg cctgaaggtg aagaagatc
//
LOCUS       DAORHD010000232         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21377_length_4378_cov_3.855887, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000232 DAORHD010000000
VERSION     DAORHD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21377_length_4378_cov_3.855887"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="PKO06_06250"
     CDS             <1..1161
                     /locus_tag="PKO06_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012570691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69282.1"
                     /translation="LVTGARQVGKTSVLRHLSQARRMYVSLDDPLVLQLAKNDPALFF
                     QRYTPPLLIDEIQYAPELLPYIKMIVDERHTSGDFWLTGSQAFHLMRGVSESLAGRTA
                     VIHLSGFSRRETALSSGAPTPFLPTRSCITKRLKATSPLPLKDLYTCIWRGSFPALIA
                     NPRMDSHLFFSSYLQTYLQRDIRDLARVGDELSFLRFIRSAAARTGQLLNLADLARDV
                     GVAFNTAKHWLSILETSGLVFLLEPYFTNVSKRLVKTPKLYFTDTGLCRYLTEWTSPQ
                     TLEAGAMAGAFFETWVVMEIVKGYQQLGIRPPLTFYRDKDGKEVDLLIVGDGTVYPLE
                     IKKTASPNQYDVRHFATLKCGPHVVGEGGIICLTKQYLPLTHAAAAIPCSAL"
     gene            complement(1167..1388)
                     /locus_tag="PKO06_06255"
     CDS             complement(1167..1388)
                     /locus_tag="PKO06_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69283.1"
                     /translation="MRDEFLSEEDLDLKHLSSAELVRVWTAWLRQAQITNDRDALEYS
                     HGVFAGADVARFFAELKRNTPDDESTSRR"
     gene            complement(1850..3484)
                     /locus_tag="PKO06_06260"
     CDS             complement(1850..3484)
                     /locus_tag="PKO06_06260"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV69284.1"
                     /translation="MTGTLHQHLETILQEATDQLTSGLNGAKGLPRQLSELARDFDQV
                     RPGVLLYGYYNSGKSTLINALLGAETASTSDSPCTDKVTEYRWQGITLLDSAGIDAPI
                     EHEQVTAEHVARFQAIAFVISTAGGSEEERTVREIERMLAWKKDVIIILNNKSGLDFT
                     KPEEQRQAIAELAALRDSLHARISWPGGRKPPIMLVNAKTALKAKLEGKTALYEMSGM
                     PDVERALVDVFRRASGNRLLMPIVERLARWFDAAEQEYDLLRTVHHDKADDLVKSLAN
                     LRRVRGELVRRFETDLVAQAGYVGRRMLNRIKQGESVDGEVDGYIRRAEGALRSRLQQ
                     ALDELGVAVNMETMGFAEAASDGGLGEGITTSSGDERPRQQNTSDENSLIRKVDPKNI
                     GHLIRSRQGEEIIRQGLLKLRGLGVPGIKGRWTLTLNRWSKNLARGVGHCVEVALMAW
                     QLAQARKAQKDYEDLMKRQHQEMVDETHQFVGHLQTTVLSQASDWVRDIFRPIEADFE
                     TSIAAAQGGSGTSQKSLAEISRLRAELEELAAHMCR"
     gene            complement(3486..>4378)
                     /locus_tag="PKO06_06265"
     CDS             complement(3486..>4378)
                     /locus_tag="PKO06_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69285.1"
                     /translation="STLLARQALDQQDHALFKESGMPELYDQLNMVMSDKAVALKQEH
                     PRRRFDLLIEDLGTSCRRLLESNEATLSELLNQKRQFQGRAQPIIRRIITKVFPDCER
                     ILNEAEEQRQPPDVWRKRLETALGECTEEVLRDEIFSFIQATGHHALSSWSQAGFRFE
                     LPDFKEVFDEIRVSKNVQGRMTGEALGAGLFGLLGSSFGPLGMMIGGYVGGKIGGAIG
                     ERNAGTETVRVAVGTNRLEIMERLIEQLDNEAAGQIIRQLNHLADEIFPPMEQQCLQL
                     RRALERLRGELVGLKFHQEN"
BASE COUNT          895 a         1374 c         1100 g         1009 t
ORIGIN      
        1 ctggtgactg gtgctcgaca ggtgggcaag accagcgttc tgcgtcatct gagccaggcg
       61 agaagaatgt atgtgagcct tgatgatccg ctggtattgc agctggcgaa aaatgatccg
      121 gctctctttt tccagcgata tacaccgccg ctcctcatcg acgagattca gtatgctcca
      181 gagctgcttc catacatcaa aatgatcgtc gatgagcggc acacgagcgg agatttctgg
      241 ctcaccggct cacaagcatt tcatctgatg cgcggagttt ctgaatcgct ggcgggtaga
      301 accgcagtca tccacctttc gggtttctcg cgccgagaaa cagctctcag ctcaggcgct
      361 cccactccct tcctcccgac cagatcatgc atcaccaaac gcctgaaagc gacttccccg
      421 ctccctctca aggatctgta cacatgtatc tggcgcggct cgtttcccgc actcatcgcc
      481 aatccgcgca tggattcgca cctctttttc agttcgtacc tgcaaaccta cctacagcgt
      541 gatattcgtg atctcgcccg ggtcggggac gaactctctt ttctacgctt tattcgctct
      601 gcagccgccc gcaccgggca gttactcaac ctcgctgatc tggctcgcga cgtgggcgtt
      661 gccttcaata ccgcaaaaca ctggctgtcc atcctggaaa cctcgggcct ggttttcctg
      721 ctcgagccct atttcaccaa cgtatcaaag cgattggtca aaactccgaa actctatttc
      781 acagataccg gcctctgccg ctacctcacc gagtggacat cacctcaaac gctcgaggcg
      841 ggcgctatgg ctggcgcctt ttttgaaacc tgggtagtca tggaaatcgt gaagggttac
      901 caacagctcg gcattcgacc accactgact ttctatcgag acaaagacgg gaaggaagtc
      961 gatctcctga tcgtcgggga tggtaccgtg taccctctgg aaatcaagaa aaccgcttca
     1021 ccaaaccagt acgatgtgag acatttcgcc accttgaaat gtggcccaca tgttgtcggc
     1081 gagggcggaa tcatctgcct gacaaagcaa tatctgccac tcacccacgc cgcagcagcc
     1141 attccatgca gtgctctatg acgcactcat cttcttgatg ttgactcgtc atcgggcgtg
     1201 ttcctcttca gctcagcgaa aaagcgagcc acgtccgccc ccgcaaacac gccgtgtgaa
     1261 tactccagcg catctcgatc gtttgttatt tgcgcctgtc gtaaccaagc cgtccacacg
     1321 cgtacgagtt cggcagacga aagatgtttg agatccagat cctcttcgct caaaaattca
     1381 tctctcatga agagcacctc tcccccggaa ccgtcggcaa gtgacgtcgg gcctgctcga
     1441 ccatcaatgt atgtgcgtcc atcaggggaa ctttgctcat gatcgccaga tgatgcaaaa
     1501 ccactgcctg accaccactg agaacagccc tggtcctccc gctgttgaag cgcttcgtta
     1561 actcaagata gatactcata gcttcgcaca ggcattctat cataccgaaa gctctttttc
     1621 tcagcgcact cctcgacagc cctgttccgt cgattcacgt tgtcacaaac gctccctatc
     1681 gtcctgtatt gagaatcagt agccatagaa tcaagtacac catcagaaga ctcctttctg
     1741 ttcacgggcg tgcccgtact ggttgcgtac aacttcgtgg gtttcgtgcg gtcactcata
     1801 tgaagttcgt acaaccgatc cggttcaata aaacatccgg ccaagagtgt cagcggcaca
     1861 tgtgggcggc gagttcttcg agttcggcgc gcaggcggga gatttcggcc agggatttct
     1921 gcgaggtccc cgaaccgccc tgcgccgccg cgatcgaggt ttcgaaatcg gcttcgatcg
     1981 gacggaagat gtcgcgcacc cagtccgatg cctgactcaa cacggttgtc tgcagatgac
     2041 cgacaaactg atgcgtctca tcgaccatct cctgatgctg gcgcttcatc aggtcttcgt
     2101 agtctttctg ggccttgcgc gcttgcgcca actgccaggc catcagcgcc acttcgacgc
     2161 aatgacccac gccgcgggcc agattcttcg accaacgatt cagcgtcaac gtccaccggc
     2221 ccttgatccc cggcaccccc agcccgcgca acttgagcag gccctgtcga atgatctctt
     2281 cgccttgtct actccgaatc agatgcccga tgtttttcgg atccactttt cgaatcaaac
     2341 tgttttcgtc agacgtgttc tgttgccgag ggcgttcgtc tccggacgac gtcgtgattc
     2401 cttcgccaag tccgccatcg gaagcggctt cagcgaagcc catggtttcc atgttcacgg
     2461 caacccccag ttcatccagt gcttgttgca ggcggcttcg caacgcaccc tcggcgcgcc
     2521 tgatgtatcc atcaacctca ccgtcgaccg actcgccctg tttgatgcga ttgagcatgc
     2581 gcctgccaac gtatccggcc tgagccacga gatcggtctc gaatcgccga accagctctc
     2641 cgcgcacccg acgcaggttc gccaggcttt tcaccagatc gtcagccttg tcgtgatgaa
     2701 ccgttcgcaa taggtcatac tcctgctcgg ccgcgtcaaa ccatcgcgcc aaccgttcga
     2761 cgatcggcat gagcagacgg ttccctgacg cccgtcggaa cacgtcgacc agtgcccgtt
     2821 cgacatccgg catgccgctc atctcataca gcgccgtttt gccttcaagt ttcgctttga
     2881 gagcggtttt cgcattcacc agcatgatcg gcggcttccg gccgccaggc cacgaaatgc
     2941 gcgcatgcag agaatctctc agagcggcga gttcggcgat cgcctgacgc tgctcttcgg
     3001 gtttcgtaaa atccaggccc gatttgttat tgagaatgat aatcacgtcc tttttccagg
     3061 ccagcatacg ctcgatctcc cgaaccgttc gctcttcctc tgacccgcct gccgtgctga
     3121 tgacgaaagc aatcgcctgg aagcgggcga catgttcggc ggtcacctgt tcgtgttcga
     3181 tgggcgcgtc gatgcccgcg gaatcaagga gcgtgatccc ctgccaccgg tattccgtca
     3241 ccttgtcggt gcatggcgag tcgctcgtcg aggccgtttc agccccaagc agggcattga
     3301 tcagcgtact cttgccgctg ttgtagtacc cgtaaagaag cacgccgggc cgcacttgat
     3361 cgaagtcgcg cgccagttca ctcaactgcc gcggcaatcc tttcgcaccg ttcagtccgg
     3421 aggtcagttg gtcggtcgct tcctgcagaa ttgtttcgag atgctgatgc agagttccgg
     3481 tcatactagt tctcctggtg gaacttcagc ccgacaagct cgccccgcag acgttcgagc
     3541 gcgcggcgaa gttggagaca ctgttgctcc attggtggga agatctcatc cgccagatgg
     3601 ttcaactggc ggatgatctg accggccgcc tcgttgtcaa gttgttcgat cagccgttcc
     3661 atgatttcaa gacggtttgt accgacggca acccgtacgg tctcggtccc ggcatttctt
     3721 tcgcctatgg caccgccgat cttgccgccg acgtaaccgc cgatcatcat tccgagcgga
     3781 ccgaaactcg atccaagaag tccaaagagt cccgcgccga gcgcctctcc ggtcatgcga
     3841 ccctgaacgt tcttcgagac cctgatttca tcaaacactt ccttgaaatc cggcaattcg
     3901 aaacggaaac ccgcctgtga ccagctggaa agcgcatgat gcccggtcgc ctgaatgaac
     3961 gagaaaatct catcgcgcag cacctcttcg gtgcactcac ccagcgccgt ctccagccgt
     4021 ttccgccaca cgtccggagg ctgacgttgt tcttccgcct cgttcagaat gcgttcgcaa
     4081 tcaggaaaca ctttcgtgat gatgcgacga atgatgggtt gagcacggcc ttgaaactgc
     4141 cttttctggt tcagcagttc tgaaagagtc gcctcattgc tctcgagaag acgacgacac
     4201 gatgtcccca ggtcttcgat cagcaggtcg aaccgccggc gcgggtgctc ctgtttgaga
     4261 gcgacggctt tgtccgacat gaccatattc aactgatcgt acaactcggg catgcccgat
     4321 tccttgaaca gagcgtgatc ttgctgatcg agagcctgcc gagcgaggag tgtcgaca
//
LOCUS       DAORHD010000233         4375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21391_length_4375_cov_3.877315, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000233 DAORHD010000000
VERSION     DAORHD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21391_length_4375_cov_3.877315"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1603)
                     /locus_tag="PKO06_06270"
     CDS             complement(<1..1603)
                     /locus_tag="PKO06_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69286.1"
                     /translation="MPAAWARRLILLLPLLTLVLLVEAGARRERISALTRFTERGQDM
                     LERLAARIDAERGVLEPLLQIGHLTASPQTRAGQPNLGLANLLSKFSGTLDLSVYFFT
                     PEGGWQGCQPEQAENQWLFKKVMAGLGQGGALDETRRKEIDRLLRSVFGFGHSLNSLL
                     LSQGRPQLVQYRNQPAWIFWERGSAGGVLVICRRWPSDLERLRGLLTSSSGNWIAGIL
                     VGFRPAGASDWSVFGAGSAGQVEDIWQQIRARGGLESDWENRRCFSRTTRGGGTLLAA
                     FPVPDSLRSRPISAGAAWSLLLGVPLILFFGFGRDRLGMLRRALIVLFLLTALVPTAG
                     LVTETLWSIDIRREVLEAEVSRAQGEALRALDQGFHDYRLRLERRLSGLLQNNTFYHR
                     DRVQVPESLKKLLEDLGGNYDLRNPMGKILEGSGGPWGLGAVANSLLQMVMETWIPER
                     LPAVRPKIDPVLDGALREQRTGFWNIAQRPQRLLELTTGKRVQFLFWHYFADPRHPAV
                     IFLSILPGENLVRHYLHQAVAERYDC"
     gene            complement(1757..3331)
                     /locus_tag="PKO06_06275"
     CDS             complement(1757..3331)
                     /locus_tag="PKO06_06275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV69287.1"
                     /translation="MNITFHGAVGCVTGSCYQVQCGETNFLVDCGMFQGSKTLKEYNY
                     RDFPFDPAKLSFVLVTHAHIDHTGMLPKLVKKGFNGKIYGLDPTVDLLRFMLPDSAKI
                     QEMEVEQKNRRNARKGVEPLVPVYSMEDAEKTLRLLYGVSENLPVNPGPGVRVTYRNA
                     GHVLGSSFIEVELTEAGKTRRVTFGGDLGGKGHPIIKDPETPTETDILLIESTYGDRL
                     HQEDSKESRLEKLAQVVQATIKRRGNVVIPSFALERTQDLLFDLLTLMDQKRLPQIPV
                     VIDSPLASESTKIFAKYPEYYDEETTELLKKQGNLFDHPNFRFTKSVEESMQLNQAER
                     TIILSSSGMCDAGRIKHHLKHQLWKKANTILFVGYQAEGTLGRLLLDGAKTVRIHGDE
                     VRVEAKIDRISGYSGHADQRELLEWLKGVTVRDRVFVIHGEDEARAEFARLVSERLQL
                     RAVIPQMGQSFDLLVEAKEPVAAPVVKHPEPVTARIDSYNLYAQLMLKTAEFMRRTSD
                     EEQRRQALEQMLKLVS"
     gene            complement(3389..3823)
                     /locus_tag="PKO06_06280"
     CDS             complement(3389..3823)
                     /locus_tag="PKO06_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69288.1"
                     /translation="MAMRPVSPELVQEMAQALGLPSLNRPHAAHVDPGDWGMVFMYIP
                     LSGEEYGKLPARLKTAVYCFGRDRYGVVVHVPKSFELPAKDKVATISAVYDQMNNLKE
                     LAYTTEENPDTRRVQDSVRRDQLLEYAKKKKIPVNVQVRSAR"
     gene            4004..>4375
                     /locus_tag="PKO06_06285"
     CDS             4004..>4375
                     /locus_tag="PKO06_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69289.1"
                     /translation="MHSLYRYLVHLLTGAFRRSRAVPGSLSAFHIATFMRRWWLPHLR
                     GLLLGAIILIPASLLSLVPSLLTLTLIDQVLLPRRSEYFVPIIVAWILVVFIDKALHF
                     CGEAWFFRLQQRFTLDLQSRLF"
BASE COUNT          906 a         1357 c         1174 g          938 t
ORIGIN      
        1 agcaatcgta gcgttcagcg acagcttggt gaagatagtg acgtaccaga ttctcgcccg
       61 gcagaatgct gagaaagatg accgccggat gacgcggatc ggcgaagtag tgccagaaca
      121 ggaactgcac gcgcttgccg gtggtaagct ccagaagccg ctgcgggcgc tgcgcgatgt
      181 tccagaatcc cgtacgctgc tcgcgcaatg cgccatccag cacagggtca atcttcggac
      241 gcacggccgg cagacgttcg ggaatccagg tttccatgac catctgcagc agactgttcg
      301 ccaccgcccc cagtccccag ggtccgccac ttccctcaag aatctttccc atcgggttgc
      361 gtaaatcgta gttgccgccc aggtcttcca ggagcttctt cagagactca ggaacctgga
      421 cacgatcacg gtgatagaac gtgttattct gcagcaatcc ggacaagcgc cgttccagac
      481 gcaaccgata gtcgtgaaag ccttgatcca gagcgcgcaa cgcctctccc tgagcgcgac
      541 tgacctcggc ttcgagcacc tcgcgccgaa tgtcgatgga ccagagcgtc tcggtgacca
      601 gaccggccgt tggtacaagc gctgtcagca gaaacagcac aatcagagca cgtcgcagca
      661 ttcccagtcg atcgcggcca aagccgaaga acaggatcag cggcactcct agaagcaggc
      721 tccaggcggc tcctgcggag atcggtcgcg accgtaacga atcgggaacg ggaaaggctg
      781 ccaggagcgt tccacccccg cgagtcgttc tcgaaaaaca gcgacggttc tcccaatcac
      841 tttcaagacc gccacgcgct cgaatctgct gccagatgtc ctcgacctgt ccggcagatc
      901 ccgcgccaaa aacggaccag tcactggctc cggcaggtct gaagcccacc aggattccgg
      961 caatccaatt gccggaactc gacgtcagta gtcccctcaa tcgttcgagg tccgagggcc
     1021 atcgacgaca gatgacgagc accccacccg cggagccgcg ttcccagaat atccaggcag
     1081 gttgattacg atactgtacg agttgtgggc ggccctgact caataaaaga ctgttaagcg
     1141 agtgcccgaa tccgaagaca gaccgtaaca gccggtcgat ctccttgcgt cgtgtctcat
     1201 cgagcgctcc cccctgcccc aggccagcca tgaccttttt gaacaaccat tggttttccg
     1261 cctgttcggg ttgacaacct tgccaacccc cttctggcgt aaaaaagtat acgctcagat
     1321 ccagggttcc cgagaacttt gacaacagat tcgccagccc caggttgggc tggccggccc
     1381 gtgtctgagg cgatgcggtc agatgcccga tctgcagcag tggctccaac accccccgtt
     1441 ccgcgtcgat gcgagctgcc agacgttcca gcatgtcctg tccgcgctca gtaaagcgtg
     1501 tgagtgccga tatccgttcg cgccgcgcac cggcttcgac cagcagtacc agtgtcagca
     1561 gcggcagaag caggatcagc cgtctggccc atgcggcggg cacggcgact gacgcgtcag
     1621 cggcttgagg gtgaattgcg ctctctcctc aggtggggtg ccacaagttg tctcgcactg
     1681 taggatagca catcacctga cacggaatag aaaaaaccgc cccaagaacc gttctcgggg
     1741 cggtcgcgcg caaaaatcag gaaacgagct tcagcatctg ctccaatgcc tgacgccgtt
     1801 gttcctcgtc cgaggttcga cgcatgaact cggccgtctt cagcatcagc tgtgcataga
     1861 gattgtacga gtcgatgcgg gcggtaaccg gctctggatg tttcaccaca ggagcggcaa
     1921 caggttcttt cgcctctacc agcagatcaa agctctgccc catctgggga atcaccgcgc
     1981 ggagctgcag ccgttcggag accaggcgcg caaactctgc ccgggcctcg tcttcaccgt
     2041 gaatgacaaa cacgcggtcg cgcaccgtca cgcccttgag ccattctaga agttcgcgtt
     2101 gatccgcatg tcccgagtaa ccggagatcc ggtcgatctt cgcttccacg cgcacttcat
     2161 cgccatgaat ccgcacggtc ttcgccccgt cgagcagcag gcgacccagc gttccttcgg
     2221 cctgataacc gacaaagaga atcgtattcg ctttcttcca gagctggtgt ttcaggtgat
     2281 gcttgatgcg cccggcatcg cacatgccgc tcgacgacag aatgatggtg cgctctgcct
     2341 ggttgagctg catcgattcc tcgaccgact tggtgaaccg aaaattgggg tgatcgaaaa
     2401 gattgccctg tttcttcaag agttccgtcg tctcttcatc atagtattcc ggatacttgg
     2461 cgaagatctt ggtcgactcg ctggccagcg gcgaatcaat cacgaccgga atctgcggca
     2521 aacgcttctg gtccatcaac gtcagcagat cgaacagaag atcctgagtg cgctcgagcg
     2581 caaaactcgg aatgacgaca ttcccgcggc gtttgatcgt cgcctgaacg acctgggcaa
     2641 gtttctcgag gcggctctcc ttgctgtctt cctggtgcag tcgatcgccg taggtcgact
     2701 cgatcaacag aatgtcggtt tcggtcggcg tttcgggatc tttgatgatg ggatgccctt
     2761 tcccgcccag atcgccaccg aaggtcacgc ggcgggtttt tccggcttcg gttaactcga
     2821 cctcgatgaa cgacgaacca agcacgtgcc cagcgttccg ataggtgacc cgaacccccg
     2881 gccccgggtt cacgggcagg ttttctgaaa ccccatacag cagtctcagc gtcttttctg
     2941 catcttccat cgagtacact ggtacaagcg gttcaacacc cttgcgggca ttgcgccgat
     3001 tcttctgttc cacttccatt tcctgaatct tggccgaatc aggcagcatg aaacgcagca
     3061 gatcaaccgt cggatcgaga ccatagatct tgccgttgaa gcctttcttc accaacttcg
     3121 gcaacatccc ggtatgatcg atgtgcgcgt gcgtgaccag gacgaatgag agcttggccg
     3181 ggtcgaaggg aaagtcgcgg tagttgtatt ccttcagcgt cttggaaccc tgaaacatgc
     3241 cacagtcgac gagaaagttg gtctctccgc actgcacctg gtagcaggaa ccggtgacgc
     3301 atccgactgc gccgtgaaac gtaatgttca tggaaaaccc ccgggaatcg aaatcagact
     3361 ctcacgccat ctcctgatga gcgatcggct acctcgcgga acgcacctgg acgttcaccg
     3421 gaatcttctt tttcttggca tactcgagca actgatcgcg tcgcacggaa tcctgcacac
     3481 gacgcgtatc cgggttttct tcggtggtat aggccagctc tttgagatta ttcatctggt
     3541 catagacagc cgaaatggtg gccaccttgt ctttggcagg cagctcgaaa gacttgggta
     3601 catgcacgac caccccatag cggtcgcggc caaaacaata caccgcggtc ttcaagcgag
     3661 cgggaagctt tccatactct tcgccgctga gcgggatata catgaacacc atgccccagt
     3721 cacccgggtc gacatgagcg gcatgcggtc ggttcaggct gggcaagccc agtgcctgtg
     3781 ccatttcttg cactaattca ggggaaacgg gtctcatcgc catgcgcgta ttctacgaat
     3841 ccccctccga cgatgcaagc tttttctggc gatcacctga caataacctg cgacgcttct
     3901 tcaggggtgc cgctgagacc acgccccggc aggttgctcc gccataggtt tcccgcaggc
     3961 ttagtgttac cattgggtat cagtcacgaa acagctcaaa gtcatgcact cactctaccg
     4021 ataccttgtc caccttctta ccggtgcctt tcgccgatcg cgggcggttc cggggtctct
     4081 ctccgccttt cacatcgcca ccttcatgag aaggtggtgg ctgccgcatc ttcgcgggct
     4141 gctcctcggc gcgatcattc tgattccggc ctctcttctt tctctggtgc cgtcgcttct
     4201 cacgctcact ctcattgacc aggttcttct gcctcgccgg tcggagtatt ttgtaccgat
     4261 tatcgtcgcc tggattctcg tcgtattcat cgacaaggcg ttgcacttct gtggagaagc
     4321 ctggttcttc cgcctgcagc aacggtttac gcttgacctg caatctcgcc tgttc
//
LOCUS       DAORHD010000234         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21426_length_4371_cov_3.775950, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000234 DAORHD010000000
VERSION     DAORHD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21426_length_4371_cov_3.775950"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKO06_06290"
     CDS             <1..538
                     /locus_tag="PKO06_06290"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV69290.1"
                     /translation="VVPREVQVPTKPTPRIAVALPEQQTIAAEPPRDSADHQLTELLA
                     QRRRERATRAAADRARVARPVTLPNVPAQSSADAVKKAEIEIQKWLRRVYRELDRRKV
                     FPEEARRQNLTGAVQGSFLVAADGSIVRADVSEDAHPVLQTSTKKLLAGLRLPPPPAA
                     WDTGSRVHCVIHYTSRGK"
     gene            542..2476
                     /locus_tag="PKO06_06295"
     CDS             542..2476
                     /locus_tag="PKO06_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV69291.1"
                     /translation="MVRVLVLVLVLFSVACGWAEEAKTYNLPELTVDAERADLGTYLP
                     FSVEQPGSLTLKLKYAPTPNEALRLNPSISISGSLKGIGGAPSIRGLPNYSNQVLLNG
                     TPVNMPWANWSNLTTFPLRRLANITVTTGGAALLHGSNGLGGAINMTLPSAADLEGLT
                     LRQEVGNQGVNHQEVIYGRVAHNNQHLFAFTTDFTRGWQRHAERDNDTFLYRGEVRTD
                     SGWKLKLDVMDFKGRMDLPDLGDRTMTQQTWDPWTIKHRDLVVEKNFGEQRQLTLRTY
                     NNWERSNSVDYADTTYSRITGQGYMDMSVKGTELLYNLTLGKHRVAAGVSQSTATQEG
                     QSVGSVERELKQTGFMLSDLMSLSDKLDLHLIGRKDKQNVAGDESSWGAHATWKPTSR
                     WRLYAGTSKMVRFPTMRELYMTFLGRQIPNPKNQNQLIWQASIPGQGDLTNRPETSKT
                     VEGAIEYRFTDSWNLRLGRFTTKADGLITQVQNPRWPQQNPRFWWRNLNQAEFTGWET
                     RLQGTVNRHCSLWIGHTRLEEADDSNTGVRLDDRPFYRLTGGFVYDRRPWQAMLSVDR
                     RGKAPYVQTGGATPLNAEIEASMRLDLSLRYQLTALAVLTLQVDNVLDADNEVNGHVF
                     GAPTIHDTPRRAVLGLECQF"
     gene            2485..3078
                     /locus_tag="PKO06_06300"
     CDS             2485..3078
                     /locus_tag="PKO06_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNV69292.1"
                     /translation="MLAINHEIRLSFEEYQSELVKAEQFHGHLCPGMYNGVKMSQLAR
                     VLLAYPSFPTKDLIVVVEIDRCLTDAICSVTGCKLGRRTLKFRDYGKFAATFCSLERK
                     QGFRLAQLESAFAAMDTEMQARGIDKHHPDAPRVFFDYPWQKQFSAAVVDVTFDDNDL
                     PGFPKERHTCTICHEPIMDGRHVVKNGKTFCRPCAGT"
     gene            complement(3098..>4371)
                     /locus_tag="PKO06_06305"
     CDS             complement(3098..>4371)
                     /locus_tag="PKO06_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV69293.1"
                     /translation="YFNRAAELITGISREQAIGKTCREIFRASICDGECALRQAIATR
                     NPVVNRAIFINTPRGNRVPISISAALLRDAHGKFIGGVETFRDLSLMEELRKDLLGRH
                     SLEDIISCNPRMKELFNLLPLAARSDSTVLVLGESGTGKELVARAIHNLSPRANKPMI
                     TVNCGALPESLLESELFGYKAGAFTDAKRDKPGRFALAEGGTIFLDEIGELTQLLQVK
                     LLRVLQEHTYEPLGSTKPQKANVRVVTATNRDLQKMVAEGKFREDLFYRINVMKIQLP
                     PLRERKDDIPLLIEHFIQRFNRIRGSRVAGISREALDLLRGFDYPGNIRQLENIIEYC
                     FILCHDEEIRPEHLPTDIRSSQPAEALPFQDLKDAEARLIISTLTRNGYNRTAAARAL
                     NMHPVTLWRKMKRYGISLRSPEVVAGRQQSS"
BASE COUNT          915 a         1251 c         1298 g          907 t
ORIGIN      
        1 cgtcgtcccc cgcgaggtcc aggttccgac aaaaccgact ccacgaattg cggttgcatt
       61 gccagagcag cagaccattg ccgctgagcc cccgcgtgac tctgcagatc accaactgac
      121 agaacttctg gcgcaacgac gaagggaaag agcgacgcgg gccgcagccg accgggctcg
      181 agttgccagg ccggtcacgt tgccgaatgt cccagcgcag tcgagcgcag atgctgtaaa
      241 aaaggcagag atcgagattc agaagtggct gagacgtgtc tatcgtgaac tcgatcgccg
      301 caaggtcttc ccggaagaag cccgtcgaca gaatctcacg ggcgcggtcc aggggtcgtt
      361 tctggttgcc gctgatggat cgatagtacg ggctgacgtg tctgaagatg cccacccagt
      421 gctgcagacg agcacaaaga aactgctcgc cggtttgcga ctgccgcctc cgcccgccgc
      481 gtgggatacc gggagtcggg ttcattgtgt catacactac acctcgaggg ggaagtgagt
      541 tatggttcgt gttctggtgc ttgtcctggt tctgttctct gtggcctgcg ggtgggccga
      601 ggaagcgaag acctacaatc tgccggagtt gactgttgat gccgagcgtg ccgaccttgg
      661 cacctacctg ccgttttcgg tcgaacagcc tggttctctg acgttgaagt tgaagtatgc
      721 gccgactccc aatgaggcgt tgcgcctcaa cccgtcgatc tcgatcagcg gcagtctgaa
      781 aggcattggt ggtgcgccgt cgattcgcgg cttgccgaac tattccaacc aggttctgct
      841 caacggcacg ccggtgaata tgccctgggc caactggtcg aacctgacga cctttccctt
      901 gcgtcgcctg gccaacatca ccgtgacaac cggtggtgcg gctctgctgc atggctcgaa
      961 cggcctgggt ggcgcgatca acatgaccct gccgtcagcc gctgatctcg aaggtttgac
     1021 cttgcgacag gaagtcggca accagggcgt gaaccatcag gaagtgatct acggacgggt
     1081 ggcgcacaac aatcagcacc tctttgcctt taccaccgat ttcacgcgag ggtggcaacg
     1141 tcacgccgag cgcgacaatg ataccttcct ctaccgtggc gaggtgcgca ccgactcggg
     1201 ctggaagctc aaactcgatg tcatggattt caagggtcga atggatcttc ccgaccttgg
     1261 agatcggacg atgacacagc agacctggga cccgtggacc atcaagcatc gtgacctcgt
     1321 ggtcgagaaa aacttcggcg aacagcgcca actgacactt cgcacctaca acaactggga
     1381 acgaagcaac tcagttgact atgccgatac gacctattct cgcatcaccg gccagggata
     1441 catggatatg tcggtcaagg gaactgaact actgtacaac ctgacactgg gaaaacaccg
     1501 cgtcgcggcg ggcgtcagcc agtcgacggc cactcaggaa gggcagtcgg tcggcagcgt
     1561 ggagcgtgag ctcaagcaga cgggtttcat gctgagcgac ctgatgtcgc tgagtgacaa
     1621 gcttgatctg catttgattg gccgcaaaga caagcagaat gtggccggcg atgaaagcag
     1681 ttggggagcg cacgccacct ggaaacccac gtcgcgctgg cgcctatacg ccggaacctc
     1741 aaagatggtg cggtttccga ccatgcgcga gttgtacatg acctttctcg ggcggcagat
     1801 tccgaatccg aaaaatcaga atcagctgat ctggcaggcg tcaattcccg gtcagggcga
     1861 tctgacgaat cgccccgaga catcgaagac cgtcgagggg gccatcgagt atcgtttcac
     1921 cgactcgtgg aatcttcggc ttggccgctt cacgacgaag gctgatggtc tgattactca
     1981 ggtgcagaac ccacgctggc cacagcagaa cccgcgtttc tggtggcgta acctgaatca
     2041 ggccgagttc accggctggg aaacccgcct gcagggtacg gtcaatcgac attgctcgct
     2101 ctggatcggg cacaccagac tcgaagaagc cgacgattcg aacactggcg tgcgtctcga
     2161 cgatcgaccc ttctatcgtc tcaccggcgg tttcgtgtat gaccgccggc cgtggcaggc
     2221 gatgctctcc gtcgatcggc gcggcaaagc cccgtatgtg cagacgggcg gtgccacgcc
     2281 tctgaatgcc gagatcgaag cttcgatgcg tcttgatttg agtctgcgat atcagttgac
     2341 ggctctcgcg gtgctgacac tgcaggtcga caatgtgctc gatgctgaca acgaagtgaa
     2401 tggtcatgtg ttcggtgccc cgaccatcca tgacacccca cgccgagccg tgctcggcct
     2461 cgaatgccag ttttgaggtg aatcatgctt gccatcaatc atgagatacg tctgagtttc
     2521 gaagagtacc agtcagaact ggtgaaagcc gaacagttcc acggtcatct ctgcccgggt
     2581 atgtataatg gtgtcaaaat gtctcagttg gctcgtgtgc ttcttgccta tccatcattt
     2641 cccacaaaag atctgattgt cgtcgtcgag atcgatcgct gtctcacgga tgcgatctgt
     2701 tccgtcaccg gctgcaaact cggtcgtcgg accctcaagt ttcgcgacta tggcaagttt
     2761 gcggcgacct tctgttccct cgaacgcaaa cagggatttc ggctcgccca gctggaaagc
     2821 gccttcgcgg ccatggacac ggaaatgcag gcgagaggca tcgacaagca tcaccccgat
     2881 gccccgcggg tgtttttcga ttacccctgg cagaagcagt tcagcgccgc agttgtcgat
     2941 gtgaccttcg atgacaacga cctgcccggc ttcccgaaag aacggcacac ctgcacgatc
     3001 tgccacgaac cgatcatgga cggtcgccac gtcgtgaaaa acggtaagac cttctgccgc
     3061 ccctgtgcag ggacttgaga cggcaacggg cgggcgttca gctcgattgt tgcctgccgg
     3121 ccacgacctc gggggagcgc agagagatgc cgtagcgttt catctttcgc cacagcgtga
     3181 cgggatgcat gttgagtgca cgagctgcgg ccgtgcgatt gtacccgttg cgcgtgagcg
     3241 ttgagatgat cagacgagct tctgcatctt tcaagtcttg aaacggcagg gcttcggcgg
     3301 gctgtgacga gcgaatgtcg gtcggcaggt gctcggggcg gatttcctca tcgtgacaga
     3361 gaatgaagca gtactcgatg atgttctcga gctggcggat gttgccggga taatcgaacc
     3421 cccgcagcag atcaagagct tcgcgagaaa tgccggcgac ccggctaccg cgaatgcgat
     3481 tgaatctctg aatgaaatgc tcgatcagca gcggaatgtc gtctttccgc tcgcgcaggg
     3541 gggggagctg gatcttcatc acattgatgc gataaaacag atcctcgcga aactttccct
     3601 cggcgaccat cttctgtaga tcgcggtttg tcgcggtgac gactcggaca ttcgccttct
     3661 gcggcttcgt cgagccaagc ggttcatagg tgtgctcctg cagcacgcgc agcagtttca
     3721 cctgcaataa ctgtgtcagt tccccgattt catccagaaa aatcgtccca ccttcggcca
     3781 gcgcaaaacg gcctggcttg tcgcgtttgg catcggtgaa tgcgccggcc ttgtagccga
     3841 acaactctga ctcgaggagc gattcgggaa gggcgccgca gttcacggtg atcatcggct
     3901 tattggcgcg aggactcaga ttgtgaatcg ctcgtgccac cagctccttc ccggttccgc
     3961 tctcgccgag cacgagaacc gtcgagtcac tgcgagctgc caggggtagc aggttgaaca
     4021 gttccttcat acgcgggtta cagctgatga tatcttcgag tgagtggcgc cccaacagat
     4081 cctttcgcag ttcttccatc aggctcaggt cgcgaaaggt ttcgacgccg ccgatgaatt
     4141 tgccgtgcgc atcacgcagg agcgcggcag aaatgctgat gggaacccga ttgccacgcg
     4201 gggtattgat gaagatcgcg cggttgacga cggggttgcg tgtggcgatt gcctgacgca
     4261 acgcgcactc gccgtcacag atactcgcac gaaagatttc gcgacacgtc ttgccgatgg
     4321 cttgctcgcg ggaaatgccg gtaatcagtt ctgccgcacg attgaagtag g
//
LOCUS       DAORHD010000235         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21427_length_4371_cov_3.688137, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000235 DAORHD010000000
VERSION     DAORHD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_4371_cov_3.688137"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="ispH"
                     /locus_tag="PKO06_06310"
     CDS             <1..680
                     /gene="ispH"
                     /locus_tag="PKO06_06310"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNV69294.1"
                     /translation="RRVKAVSEVTSGGTLIISAHGVSRDVYQAMEQSGISCIDTTCIF
                     VKKAQDAAAFLCREGYEVFLVGDPDHTEVKGIVSAAGGPVRILQRQEDLETVTRGKRA
                     GLLFQTTQTAEMLRLFTSGLAEKFDEVRVFNTICRATTERQEAVRALARDVDAVVIVG
                     DRKSANSKRLLAIASEINPRSFMVENADQINVNDFTSLDTVGLTASASAPDWVIAEVR
                     TRLESLP"
     gene            complement(682..1659)
                     /locus_tag="PKO06_06315"
     CDS             complement(682..1659)
                     /locus_tag="PKO06_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69295.1"
                     /translation="MEHLESLIALIQRLQSLHPIGGLPKGPIRQAELSPDFRSLVTSR
                     EPWRSQVLAEARWHFAFPRLTGIGMEVRLSAPLVPSAFEDWALSISPVEAELTWSRDM
                     SGQSSDRCTYEIQRIAWEDDAWKVVELWSPRARKSNLAFIEMLNGKGSQEVASNATSL
                     AADPHPDKGASESPSSATAESVLPASAEDSPKASRWRRIGKWLLILGLIWAGTTTSVT
                     VKTRSNHSWVASPGSISESTRVYWVWPGQSFGSSTGMPMMVFGKPLFRLIVPGIWSAT
                     ISYSEDLSCSAPSEGQGNVVTIYREPIRFYTRTKDLGSTIEVSLGGYFW"
     gene            complement(1789..2511)
                     /gene="nusB"
                     /locus_tag="PKO06_06320"
     CDS             complement(1789..2511)
                     /gene="nusB"
                     /locus_tag="PKO06_06320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNV69296.1"
                     /translation="MSSRRRRAREAALKALYQIDLTGVEATTSLHDVMADEIYLPAFE
                     SFAREFLTRIATDRAGDSTAESFARAFAHEVVMGTAPFPPDAEALRAAVKQAAAAVDL
                     ELDPSDAALGMLYKRCAEKVESLKDLEAFARLLVQRTVEHRAELDTLLSKFTDNWSLD
                     RMASLDRSILRYAVCELVHFRDIPINVTINEAIELARKYSTDRSCEFVNGVLDKISRE
                     LKPEKDDPRARKKDTATGEGDS"
     gene            complement(2520..>4371)
                     /locus_tag="PKO06_06325"
     CDS             complement(2520..>4371)
                     /locus_tag="PKO06_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNV69297.1"
                     /translation="DQNKHLHSLEEEMPSTYKQLVEIRNKLESHYKDMQDIEFTIQEG
                     KLYMLQCRSGKRTGTAALNMAMDMLAEKLISEEMAVLRVEPNQLDELLHPIVDPSAEK
                     KAEPVAKGLPAGPGGACGQIVFTAADAVAWKKQGKKVILVREETNPEDVEGMRAAEGI
                     LTARGGMTSHAALVARGWGKCCIVGAGMLHPDVHSKTLKIQGSKHSFKEGDFITLNGT
                     AGKVYEGKIQMMDASENPRFQKFMAIVDKNRKMQVWTNAESPKDAKLARQFGAEGIGL
                     FRTEHMFYGEGSDKPLFFLRKMILSSNVEERKNALSELYPFVKKDIKGTLEEMDGLPV
                     TFRLLDPPLHEFVPQAEDKQAELAKSLGIKTADVKKRGEALHESNPMMGHRGVRLGIT
                     YPEVSEMQIRAIFEATAELLKDGKHPKPEIMVPVTCDVRELDFTKKIVDRVYEEVVKA
                     TGVKKIDYHYGTMIEIPRAALMGDRMAKTCEFFSFGTNDLTQMGFGFSRDDTGAFMHD
                     YLEHRILDNDPFQTIDQEGIGQLIQICVERGRKARPDLKVGICGEQGGEPASVEFCFK
                     TGLDYVSCSPFRVPIARLAAAHASLKHKELREKVLKSQGHTPLKALHAGK"
BASE COUNT          859 a         1293 c         1287 g          932 t
ORIGIN      
        1 tgcgacgggt caaggccgtg tccgaggtga cgtcgggtgg aacgctcatc atcagcgcgc
       61 atggcgtctc acgagatgtg tatcaagcca tggagcagag tgggatctcg tgcatcgaca
      121 ccacctgcat cttcgtcaag aaggctcagg atgccgcggc gtttctctgc cgcgaagggt
      181 atgaagtctt tctggttggc gatcctgatc acaccgaagt caagggcatc gtctcggcgg
      241 ccggcggccc ggtgcgtatt ctgcagcgcc aggaagacct cgaaaccgtc acccggggca
      301 aacgagccgg cttgctcttt cagaccacgc agacggctga gatgctgcga ctgttcactt
      361 cgggtctggc cgagaagttt gacgaagtac gcgtcttcaa tacgatctgc cgcgccacca
      421 ccgagcgaca ggaggccgtt cgcgcactcg cgcgtgatgt cgatgccgtg gtcatcgtcg
      481 gtgaccgcaa atcggccaat tcgaaacgat tgctggccat cgcctcagaa atcaacccca
      541 ggtcattcat ggttgaaaac gctgatcaga taaacgtgaa cgacttcacc agcctggaca
      601 ctgtcggtct gactgccagc gcctcagccc ctgactgggt catcgcggaa gtgcgaaccc
      661 gcctcgagtc attaccatga gttaccagaa gtagccgccc agcgagactt cgatggtgct
      721 tcccagatcc ttggtccgcg tgtagaagcg aatcggttcg cggtagatgg tcaccacgtt
      781 gccttgacct tcggaaggcg cagagcagga aaggtcttca gaatacgaga tcgtggcgct
      841 ccagattcct ggaacgatca atctgaacag cggtttgccg aacaccatca ttggcatgcc
      901 ggtcgaactg ccgaatgact gccccggcca gacccagtag accctggtgc tctcagaaat
      961 agagcctggt gaggcaaccc agctatgatt gctccgcgtc ttcaccgtca cggatgtggt
     1021 cgtacctgcc cagatcaaac caagaatcag cagccacttt ccgattcttc gccagcgcga
     1081 agctttcggc gagtcttccg ccgaggcagg gagcacagat tctgcggtcg cagaagaagg
     1141 actttctgac gcccccttgt cagggtgagg gtcagccgca agactcgtcg cattcgaggc
     1201 aacttcctgt gacccttttc cattcagcat ctcgatgaaa gcaaggttgg actttctggc
     1261 cctcgggctc cacaactcca cgactttcca ggcatcgtct tcccaggcaa tccgctgaat
     1321 ctcataggtg cagcggtctg aagactgtcc gctcatgtcc cgagaccacg tcagttctgc
     1381 ttcaaccggt gaaatggaga gcgcccagtc ttcgaaggcc gacgggacca gtggggctga
     1441 caagcgaact tccatgccga ttccggtcag acgcggaaaa gcgaagtgcc agcgggcttc
     1501 agcgagaacc tgcgaacgcc acggctcgcg gctggtgacc aaagatctga aatccggcga
     1561 aagctccgcc tgccgaatcg gccccttcgg caaaccaccg atcggatgca ggctttgcag
     1621 cctctgaatc agcgcgatca gactctctag atgttccatt cccctcaccg cgcagagatc
     1681 ctctggaaac agggactcgc accctgcgct gtttcgagcc cggcaccgcg ttcagctgca
     1741 gtcccgggtg ccccggcata cccactcact caggctatca ccgcgaagtc agctgtcgcc
     1801 ctctcccgtc gctgtatcct tcttgcgggc gcgggggtcg tctttttcgg gtttgagttc
     1861 gcgagaaatt ttgtcgagaa ccccgttgac gaactcgcag ctgcggtcgg tcgaatattt
     1921 gcgagccagt tcgatggcct cgttgatcgt aacgttgatt ggaatgtcac gaaagtgtac
     1981 aagctcgcac actgcataac gaagaattga acgatcaaga ctggccatac gatcgagaga
     2041 ccagttgtcg gtgaatttcg acagcagggt gtcgagttcg gcgcgatgtt caacggtgcg
     2101 ctgtaccagc aaccgggcaa acgcctcgag atctttcagg gattcgactt tctcggcaca
     2161 gcgtttgtac aacataccga gggccgcatc gctggggtca agctcaagat cgacggctgc
     2221 cgccgcctgt ttcaccgccg cgcgcaacgc ctcggcatcg ggcggaaacg gggcggtgcc
     2281 catgacgacc tcgtgcgcaa aagcccgggc aaaggattcc gcggtggaat cccctgcccg
     2341 gtcggttgcg atacgagtca ggaactcccg agcgaaggac tcgaaagccg gcaagtagat
     2401 ctcatcggcc atgacatcgt gaagcgaggt cgtcgcctca actccggtca ggtcgatctg
     2461 gtacaatgcc ttcagagcag cttcacgagc ccttcgacgt cgggatgaca tatcaggcct
     2521 tacttaccgg cgtgcagcgc tttgagcggg gtgtggccct gggacttcaa caccttctcg
     2581 cgcagctcct tgtgcttcag cgacgcatga gccgccgcca gacgggcgat aggcacgcgg
     2641 aacggagagc agctgacgta gtcgaggccg gtcttgaagc agaattcgac cgaggccggt
     2701 tcgccaccct gctcaccgca gatgccgacc ttcaggtcgg ggcgggcttt gcgaccacgc
     2761 tcgacgcaga tctgaatcag ctggccgatg ccttcctggt cgatagtctg gaacggatcg
     2821 ttgtcgagaa tacgatgctc gaggtagtcg tgcatgaacg caccggtatc gtcgcggctg
     2881 aacccgaagc ccatctgggt caggtcgttg gtgccgaatg agaagaattc gcaggtcttg
     2941 gccatgcgat cgcccatcag agcggcgcga ggaatttcga tcatcgtgcc gtagtggtag
     3001 tcgatcttct tcacgcccgt cgccttcacg acttcttcgt agacgcgatc aacgatcttc
     3061 ttggtgaagt cgagttcacg cacgtcgcag gtcaccggaa ccatgatctc gggcttcgga
     3121 tgcttgccgt ctttcaacag ttcagccgtc gcctcgaaga tggcgcgaat ctgcatttcg
     3181 gagacttcgg gataggtgat gccgaggcgg acgccgcggt gacccatcat cgggttgctt
     3241 tcatgcagag cttcgccgcg cttcttgaca tcggcggtct tgatgccgag actcttggcc
     3301 agctcggcct gcttgtcttc agcctgcgga acgaattcgt gcagcggcgg atcaagcaga
     3361 cggaaggtga ccgggaggcc atccatctct tcgagcgtgc ccttgatgtc tttcttcacg
     3421 aaggggtaca actcactgag cgcattcttg cgctcttcaa cgttgctgga gaggatcatc
     3481 ttgcgcagga agaacaacgg tttatcggag ccttcgccgt agaacatgtg ttcggtgcgg
     3541 aacaggccga tgccttcagc gccgaactgg cgggccagct tggcgtcttt gggtgactcg
     3601 gcgttcgtcc agacctgcat cttgcggttc ttgtcgacga tggccatgaa cttctggaag
     3661 cggggatttt ccgacgcatc catcatctga atcttgcctt catacacctt gccggcagtg
     3721 ccgttcaggg taatgaaatc gccttccttg aaggaatgct tggagccctg gatcttcagg
     3781 gttttgctgt gaacgtcagg atgcagcata ccggcaccga cgatgcagca cttgccccaa
     3841 ccgcgggcca cgagagccgc gtgggaggtc atgccaccgc gggcggtgag aatgccttcg
     3901 gccgcgcgca tgccttcgac gtcttcgggg ttggtttctt cacggaccag aatgactttt
     3961 ttgccctgtt ttttccaggc aacggcatca gccgcggtga acacgatctg accgcaggcg
     4021 ccgccgggac cggcaggcaa acccttggcg accggctcag ccttcttttc ggcggacggg
     4081 tcgacgatcg ggtgcagcag ctcatcgagc tggttgggtt caacgcgcag cacggccatc
     4141 tcttcgctga tcagcttctc ggccagcata tccatggcca tgttcagggc ggcggtaccg
     4201 gtgcgcttgc cgctgcggca ctgcagcatg tacagcttgc cttcctggat cgtgaactcg
     4261 atgtcctgca tgtctttgta atgcgactcg agcttgttcc ggatctcgac gagctgcttg
     4321 taggtcgagg gcatctcctc ttccaggctg tggaggtgct tgttctggtc g
//
LOCUS       DAORHD010000236         4366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21469_length_4366_cov_3.382046, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000236 DAORHD010000000
VERSION     DAORHD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21469_length_4366_cov_3.382046"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            320..1168
                     /gene="fliP"
                     /locus_tag="PKO06_06330"
     CDS             320..1168
                     /gene="fliP"
                     /locus_tag="PKO06_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756332.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNV69298.1"
                     /translation="MFSRGKILLLLTLILFPLLPCPVFGQTHSVSNPVARINTGDSSK
                     VEKIEPTETPFPIPRIRIEVDPASERSQIGTSLQILILLTILSLSPSILIMLTSFTRI
                     LIVLNFVRRALTTQQEPSNQILIGLALFLTFFTMTPVINKVNETAVRPFIAREIDYVE
                     AYNRAIQPVRDFMFLHTRKSDLALFASIEGSPKPQTRNEYSTLALIPAFMTSEIRTAF
                     QMGVIIFLPFLIIDLVVASILMSMGMIMLPPAMISMPFKILLFVLVDGWNLVIKSLVT
                     SIGAIS"
     gene            1165..1434
                     /locus_tag="PKO06_06335"
     CDS             1165..1434
                     /locus_tag="PKO06_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HNV69299.1"
                     /translation="MTEFTLLQLLRETIYLSFMLSLPLLAIGTVIGVLVSIVQTATSI
                     QDQSLSFIPKLLATALGLVFFGPWMLSLVMNFTTRLLSTLHTFAR"
     gene            1438..2220
                     /gene="fliR"
                     /locus_tag="PKO06_06340"
     CDS             1438..2220
                     /gene="fliR"
                     /locus_tag="PKO06_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNV69300.1"
                     /translation="MTEAFFLRAVGTFLISLVRISGALINLPAFGEGVVLNRVKAGIA
                     ALMSLLLLPHLMRTQPLPSLGILDYGVMALKELALGLTLGYIVLIIIDTLKFAGELIG
                     MQIGFSFVQVVDPESSRGQAIMAEFFQILGVLTFLLMDGHLIFLGAFMQSFDLVPLAG
                     MVFNSSMATELARLTAGVFLIGLQLSMPIIGIILISDAALGIIARTVPRMNVFQVGFA
                     LKISLGLWVITMILPFISDKVHRLFDQGYQTIGLFMRLMSGA"
     gene            2217..3389
                     /gene="flhB"
                     /locus_tag="PKO06_06345"
     CDS             2217..3389
                     /gene="flhB"
                     /locus_tag="PKO06_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNV69301.1"
                     /translation="MRVTDFSATGFSGASSTFVPVTKRHHALSMRDLPFDLQWFAGEK
                     TEPATDKRREEARERGQVAKSQDAEATAVLFASLVCLYYFGPQMFGVITDFMRYCLSF
                     AAGTALQTGTTTILLHQFLWVFLKAMAPLFVVVAFTAIAANLAQVGFLFSTEPLVPDL
                     NRINPINGLENIVSWKAVAELVKSIFKIAAVAYIPYSTLRDQMPFVLRFIQLDPFPAT
                     RFLLEIIFWMAMKILLLLALLAIADYFFQWWRLEESLKMSKEEIKEEYKQREGDPKVK
                     AKIRERQRRMAQARMMQEVPKATVVVTNPTHLAVAIVYDRDNATSAPRVVAKGAGLIA
                     QKIKEIAKEHDVPTIENKPLARALHEKVEVGEEIPVELWETVAMVLAEVYRLKGMV"
     gene            3495..>4366
                     /locus_tag="PKO06_06350"
     CDS             3495..>4366
                     /locus_tag="PKO06_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005490024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNV69302.1"
                     /translation="MAEVRTSPVNLVPFADILKNRDVIFSFGIVSIVIMMVVPLPPAM
                     LDVLMALNIALSVTILLVTLYTKEALEFSAFPSLLLIATLFRLALNVSSTRLILLGQG
                     SQISIVTAFGNFVVGGNYVVGLVVFAILAIFQFIVITKGSERVAEVAARFTLDALPGK
                     QMAIDADLNAGVIDQDAARIRRRNVQREADFYGSMDGASKFVKGDAIAGIIVIFVNIL
                     GGLLIGVIMRGESLADAAQLYILFTVGDGLAAQIPSFLISTATGIVVTRSSSDMNMGE
                     ELSGQLGAQPKVLA"
BASE COUNT          863 a         1298 c         1108 g         1097 t
ORIGIN      
        1 accggtcctc tttccggctt tccccacccc cacgcaatcc tgcgctcatt ctctctcctt
       61 gccgacttcg tgcgtcaagc atcgtcatct cttctcgcgc acagtgtgct tttgcagtga
      121 aatcacctca tgaaacaccc gggagaacag gcagatttcc gagggacatt tcgcaggtgt
      181 aagctgatgg tttcggatcg gtgccctctt ggaatcgcta cgatcgacat cggtgccccg
      241 tgcgaattgt acgcacggct tttttcatct ttgcgtcttg ccaactaacg ttttttccat
      301 tattaacttg atatccctta tgttttcacg cggaaaaatc ctgcttctgc tcacgctgat
      361 tctgtttcct cttttgccgt gccctgtgtt cgggcaaacg cacagcgtca gtaacccggt
      421 tgcacgaatc aatacgggtg attcttccaa ggtcgagaag atcgagccca ccgaaactcc
      481 atttccgatt ccccggattc gcatcgaagt agaccctgcc agcgagcgca gtcagatcgg
      541 cacgtcgctc cagatcctca ttctgctgac catcctcagc ctctcgcctt cgattctgat
      601 catgctcacg agcttcaccc gcattctgat cgtattgaac ttcgtgcgcc gagccctcac
      661 cacgcaacag gagcccagca accagattct gatcgggctc gccctgtttc tcacgttctt
      721 cacgatgact ccggtcatca acaaagtgaa tgagacagcc gtgaggccat tcatcgcccg
      781 cgagatcgac tatgttgaag cctataatcg agcgatacaa cccgttcgcg acttcatgtt
      841 tctgcacacc cgcaaatctg atctggcgct cttcgccagc atcgaaggca gccccaagcc
      901 gcagacacgc aacgaataca gcacgctcgc cctgattccc gcgttcatga cgagcgagat
      961 tcgaaccgcg tttcagatgg gcgtcatcat ctttctcccc tttctgatca tcgatctggt
     1021 cgtcgcgagc attctcatgt ccatgggtat gatcatgctg cccccggcga tgatctcgat
     1081 gcccttcaag attctcctgt tcgttctggt cgacggctgg aacctcgtga tcaagtcgtt
     1141 ggtcaccagt atcggtgcca tctcatgacc gaattcacgc tgctgcagct gctgcgcgag
     1201 acgatctatc tgtcgtttat gctgtcactg cccctcttgg cgatcggcac ggtgattggt
     1261 gtcctggtca gcatcgtgca gaccgccacc tcaatccagg accagagtct gtccttcatt
     1321 cccaagttac tggcaaccgc cctggggttg gttttcttcg gcccctggat gctgtcactg
     1381 gtcatgaact tcacgacccg ccttctctcg accctgcaca cgtttgcccg ataagccatg
     1441 accgaagcgt tttttcttcg ggccgtcggc acctttctca tttcgctggt gcggatttct
     1501 ggcgccctga tcaatctgcc ggcgttcggc gaaggcgttg ttctcaatcg ggtaaaagcg
     1561 ggaatcgcag ccctgatgag ccttttacta ctgcctcatc tcatgcgcac ccaaccactg
     1621 cccagtctcg gcattcttga ttacggagtc atggctctca aagaactggc attggggctg
     1681 acactcggct acattgtgct gatcatcatc gacaccctga agttcgccgg cgaactcatc
     1741 ggcatgcaga tcggtttttc atttgtccag gttgtcgacc cggaatccag ccgtggacag
     1801 gccattatgg ccgagttctt tcagatactc ggtgttctga cttttctact gatggatgga
     1861 catctgatct tccttggggc attcatgcaa agcttcgacc tcgttcccct ggcagggatg
     1921 gtcttcaact cttccatggc gaccgagctt gcccgcctca cggccggtgt ctttctgatc
     1981 gggcttcagt tgtcgatgcc catcatcgga atcattctga tcagcgacgc ggctctgggc
     2041 atcatcgccc gcaccgtgcc acgtatgaat gtgtttcagg ttggtttcgc cctgaagatc
     2101 agtctcggac tctgggtcat cacgatgatc ctgccgttca tttccgacaa agtgcacagg
     2161 ttgttcgatc agggctacca gacgatcggc ttgttcatgc gcctgatgag cggcgcatga
     2221 gagtgacaga tttttccgcc accggttttt ccggagcgtc gtcgactttt gtcccagtca
     2281 ccaaacggca tcatgctctt tccatgcgcg atcttccctt tgacttacag tggtttgccg
     2341 gggaaaagac cgaacccgcc actgacaaac gccgtgagga agctcgcgaa cgtgggcagg
     2401 ttgccaaatc gcaggatgca gaagccacgg cggtgttgtt tgcttcgctg gtctgtttgt
     2461 actacttcgg cccacagatg ttcggcgtca ttaccgactt catgcggtat tgtctgagct
     2521 tcgctgccgg caccgcgctg cagaccggaa cgaccaccat cctgctgcac cagtttctct
     2581 gggtgttcct gaaggcgatg gctccgctgt tcgtggttgt cgctttcacg gcgattgccg
     2641 ccaatctcgc tcaggtcggc tttctgtttt cgaccgaacc gctggtgccc gatctcaatc
     2701 gcatcaatcc gatcaacggg ctcgagaaca tcgtctcctg gaaagcggtc gcagagctgg
     2761 taaagtcaat tttcaaaatt gccgcggtgg cctacattcc gtattcgacc ctgcgcgacc
     2821 agatgccttt cgttcttcgc ttcattcagc tcgacccgtt tccagcgaca cgttttctgc
     2881 tcgagatcat cttctggatg gccatgaaga ttctgcttct gctcgcgctc ctcgctatcg
     2941 ccgactactt ttttcagtgg tggcggctcg aagaaagcct gaagatgtcc aaagaagaga
     3001 tcaaagaaga gtacaaacaa cgggaaggag acccgaaggt caaggccaag atccgcgaac
     3061 ggcagcggcg catggctcag gcacgcatga tgcaggaagt cccgaaagcc acggtggtcg
     3121 tcaccaaccc gacgcacctg gccgtggcta ttgtgtatga ccgcgacaac gcgaccagtg
     3181 ccccgcgcgt cgtcgccaag ggagcaggtt taatcgctca gaaaatcaag gaaattgcca
     3241 aagagcacga cgtgcccacc atagagaaca aaccgctggc ccgtgcccta cacgagaaag
     3301 tcgaggtcgg cgaggaaata cccgtggaat tgtgggaaac ggtcgcgatg gttctcgccg
     3361 aggtgtatcg gttgaaggga atggtgtaat gattcatgtg aaaccgaaaa actgtgtaaa
     3421 aattgcctct tttttccctg tgccccatca ggtataatac gcgcacacac cgctgagggg
     3481 aaggaatcta caccgtggcc gaagtacgca ccagtcctgt gaacctagtg cccttcgccg
     3541 acatcctgaa aaatcgggat gtcatctttt ctttcggcat cgtcagcatc gtcatcatga
     3601 tggtcgtgcc gctgccgccc gccatgctcg atgtgctcat ggccctgaac atcgctcttt
     3661 ccgtgaccat tctgctcgtc actctgtata ccaaagaagc gctcgaattc tccgcctttc
     3721 ccagtcttct gctgatcgcc acactattcc gcctggctct gaatgtcagt tccacacgtc
     3781 tgattctgct tgggcaaggc tcacagatca gcatcgtcac ggcattcggc aacttcgtgg
     3841 ttggcgggaa ctacgtggtt gggttagtcg tgttcgccat tctggccatt tttcagttta
     3901 ttgtcatcac caagggctct gagcgcgtcg ccgaagttgc cgcacgtttc accctcgatg
     3961 ccttgccggg caaacagatg gccatcgatg ccgacctgaa tgccggagtc atcgatcagg
     4021 atgccgcccg cattcgacga cgcaatgtgc aacgcgaagc cgatttttac ggttccatgg
     4081 acggcgccag caagttcgtg aagggtgacg ccattgccgg cattattgtc atcttcgtga
     4141 acattcttgg cgggttgctt atcggcgtga tcatgcgcgg agaaagcctg gccgacgctg
     4201 ctcagttgta tattctgttc actgttggcg acgggctggc cgcacagatc ccctcattcc
     4261 tgatctcgac ggcaaccggt atcgtcgtca cgcgttcgag ctcggacatg aacatgggcg
     4321 aggaactcag tggtcagctc ggtgctcaac cgaaggttct ggccat
//
LOCUS       DAORHD010000237         4363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21494_length_4363_cov_3.691504, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000237 DAORHD010000000
VERSION     DAORHD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21494_length_4363_cov_3.691504"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="PKO06_06355"
     CDS             complement(<1..344)
                     /locus_tag="PKO06_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV69303.1"
                     /translation="MTNGHDEVIDLGLLGKLLKQLEESTVGTVIAKLDPRIINELREL
                     IRFMHRREPRIMLVGRRGAGKSSLVNAMLGVKARNVGSVTVGSLDCKWEDVGPEEKRL
                     RVLDTKGTGQGD"
     gene            678..1040
                     /locus_tag="PKO06_06360"
     CDS             678..1040
                     /locus_tag="PKO06_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69304.1"
                     /translation="MSNVLCITHQMSEAEVRIAMADVAAILQTRFPACQTNVFQPRSP
                     IFLKVADSGVQLVYQPQRLLLKYWHPDAPPPPLAFSTVAMHLIKHAVWHWHNETALSD
                     TPCRANWQRFWPSNAPPQ"
     gene            1176..1841
                     /locus_tag="PKO06_06365"
     CDS             1176..1841
                     /locus_tag="PKO06_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69305.1"
                     /translation="MNRHHEREMHPWRYALEDTGYPLAERILDLRLNRERWSEKGAWQ
                     LVPISVFDGLLRSAMQRISPELRLLLFQRLTEGNCPWEALAIARIEQDPRTRIQLLKG
                     LQTRTPARIGKVLSRELDRLQQLYPALFAKTTPWSFAESPAPDAATEHPTRAPSLGNR
                     RECSECSFLAAGSQPDSSRAECPQRAQQSPAGDSARTAREPFPEKASPIALLECPRSA
                     SVH"
     gene            1729..4059
                     /locus_tag="PKO06_06370"
     CDS             1729..4059
                     /locus_tag="PKO06_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69306.1"
                     /translation="MLSNLPPEIPREPLENLFLKKHHPLRFSSAREALQYIEWCAFLR
                     HVPPAAMKEHIGTDARSMRALFITGWDKTPYLDVFKNLDPSKQMTELEFFRDWNLPID
                     DLRSFMLPEVVTWYDCLSLPANATRGALRSTFYACIRRSFQTIREYHLFRCAFLTVLS
                     DSDHLAPEYDEWLARQRADAAEKALSKVSEADLNDLKDPVILADSIFSAATTGVLSVA
                     PAQVRLLLLNRLVSNDIRFLFHGLIQLVRAGLLTEAAENRHRIESDLLEIEGFLLQEA
                     AGIEHLHREVEELLREQSFQQALTTLTSLPSPSASVLESAIQASIGSGDLNAAGGFLR
                     RLPEDYRTRTFWKNLIDSFETEPSVGLPALARVLADAGSDREAWAFRALADLLTRTRR
                     EIPAPSWFAGLSVSSENLRSLIDLVLYHAGYLDEPRRQSFIAHPLAQRLARQWGWKLD
                     IMKSADRSPGWVRAWADLLDGKPARPAMRCDLRLQKLLTSNKWNHAGDLGFRLVHEQA
                     SVPELVQETDLWRQSEGFRARAFQFVAARVSPCSSDGRKAIELAASRPEAIVEFMLSS
                     AEPLSELSVRLKSIDTSSQTFASLFLKMLDYIRRTGDDEFARQLGEASPIAWWRLHAA
                     PPTSFAEREELRRHLQLVNTELDRWRETDERQRALTSPDAWQIKATKLFLTALDEFRE
                     SNPGLVEWAVKHIVDLATSADARHRLPISKTNSSLKAVGRSKQDGRLLFTLDGKSREL
                     RLYRLYPVQHHDAYERDLSKPEISGELLVTVPDLMG"
BASE COUNT          860 a         1407 c         1143 g          953 t
ORIGIN      
        1 tcgtcaccct gcccggtgcc cttcgtgtcg agcacgcgca gacgcttctc ctccgggccg
       61 acgtcctccc atttgcagtc aagagagccg accgtgaccg atccgacgtt acgggctttc
      121 acgcccagca tcgcgttgac cagcgacgac ttgcccgcgc cgcgccgccc cacgagcatg
      181 atgcgcggct cgcgccgatg catgaaccgg atcagttcgc gcagttcgtt gatgatccgc
      241 gggtccagct tcgcgatgac cgtcccgaca gtcgactcct ccaactgctt cagcagtttt
      301 cccaaaaggc ccagatcgat cacttcatca tgtccgttcg tcatacgttg ttctcctcag
      361 atcgttcaca agatttttcg cgcacctatt cgggagaagg gcgttgccag ccctcggttc
      421 aacaatcaat cccgttttcc tctgcctgcg agtctgtttc cttgtttttg cgttcggtca
      481 tggcgaacct cctgttggtt ttcattggtt tacatccaca gaaagccgcc accgcaataa
      541 cggttcaaaa tctcggcagt gccgctccaa ttcgccgttc cagatattga agccgcttct
      601 tcgtcacccg ttcacattgt tgaaccgaca tcactgtatc gactttgtgg tgagagaaat
      661 cacacgcagg aggagttatg agcaacgttc tgtgcatcac ccatcagatg agtgaagccg
      721 aggtccgaat tgccatggcc gacgtggcgg ctatccttca gacacgcttt ccggcatgtc
      781 agaccaatgt tttccaaccg cggagcccca tctttctgaa ggtggccgat tccggagtcc
      841 agctggtcta tcaaccacag cgactgcttc ttaagtattg gcatccggat gcccccccac
      901 ctccccttgc cttctccacc gtcgccatgc atttgataaa acacgctgtc tggcactggc
      961 acaacgaaac cgcgctttcc gataccccct gccgggcgaa ctggcaacgc ttctggccga
     1021 gcaacgcgcc accacagtga cctcgagtcc cgacctctca gacgcgactg aacaacttga
     1081 ccgcccgaat tcatttgttt actcttagtc gaccgaacaa gccaacacgc gttctcacgc
     1141 aaaccggaac cagagagcaa cagaggagat cgcagatgaa tcgtcatcat gagcgggaaa
     1201 tgcatccctg gcgctacgcg ctcgaagata cggggtaccc tcttgccgaa cggattctcg
     1261 atcttcgtct gaaccgcgag cgctggtcgg aaaagggagc gtggcaactg gttcccatct
     1321 ctgtgttcga cggtctcctt cgaagcgcca tgcagcggat ctctcccgaa ctgcggctct
     1381 tgttgtttca acgactgacc gagggaaact gcccctggga agctctggcc atcgccagga
     1441 tcgaacagga cccccgcact cgtatccagt tgctgaaggg tcttcagact cgcacaccgg
     1501 cgcgaatcgg aaaagttctc tcgcgcgagc tcgaccgact gcaacaactc tatcccgctc
     1561 tcttcgcgaa aaccacgccc tggtcgttcg ccgaatcgcc cgcccccgat gcagccacag
     1621 agcaccccac ccgcgctccc tccctcggaa accgccgcga atgttctgaa tgttcgttcc
     1681 tggccgcagg cagccagcct gattcgtctc gggcagaatg cccgcagcgt gctcagcaat
     1741 ctcccgccgg agattccgcg cgaaccgctc gagaaccttt tcctgaaaaa gcatcaccca
     1801 ttgcgcttct cgagtgcccg agaagcgctt cagtacattg agtggtgcgc atttctccgg
     1861 cacgttcctc cggccgccat gaaggaacat attggcacag atgcccggtc gatgcgtgcc
     1921 ctgttcatca cgggctggga caaaacgccc taccttgacg tgttcaagaa tcttgatccg
     1981 agcaagcaga tgaccgaact cgagtttttc cgggattgga atctgcccat cgacgatctt
     2041 cgttctttca tgcttcccga ggttgtcacc tggtacgatt gtctgtccct tccggcgaat
     2101 gcgacccgcg gggctttgcg gtcgacgttt tatgcctgca tcaggcgcag ttttcaaacc
     2161 atccgcgagt atcacctctt ccgttgcgcc ttcctgactg ttctttcgga tagcgaccac
     2221 cttgccccgg agtatgacga atggctggcg agacagagag cggacgccgc tgagaaagct
     2281 ctgagtaagg tgtcggaagc tgatttgaac gatctcaagg atcctgtcat tctcgccgat
     2341 tccattttct ctgccgcgac aaccggggtg ctttccgttg ccccagctca ggttcgcttg
     2401 ctccttctga atcgcctggt cagcaacgat attcgctttc tctttcacgg cctgattcag
     2461 ctcgtccgcg ccgggctcct gaccgaagcc gccgaaaacc ggcaccgtat tgaaagcgat
     2521 ctgctcgaaa tcgagggatt tctcttgcag gaagcggccg gcatcgaaca tcttcatcgc
     2581 gaagtcgagg aactgcttcg cgaacagtcc ttccagcagg ccctgaccac gctcacaagt
     2641 ctcccgagtc ccagcgcatc cgttcttgaa tcggcaattc aggcgagtat cggaagcggc
     2701 gacctcaatg ccgcgggtgg ttttctccgc cgacttcccg aggattatcg cactcgcacc
     2761 ttctggaaga atctgattga tagctttgag acagaaccat ccgtcggcct gcctgccctc
     2821 gctcgtgtcc tggccgatgc cggttctgat cgtgaagcct gggccttccg cgcgttggca
     2881 gatcttctga cccgcacgcg cagagagatc ccagccccgt cctggtttgc cggtctgtcc
     2941 gtatcatcag agaatctccg ttccctgatc gatctcgtgc tctatcatgc cggctatctc
     3001 gacgaaccgc ggcgccagag ctttatcgcg catcctcttg cccagcgtct ggctcggcag
     3061 tggggttgga agctggacat catgaagtcg gcagaccgaa gccccggctg ggtgcgcgcg
     3121 tgggccgatt tgctggatgg gaaacctgcc agacctgcca tgcggtgcga cctgcgtctg
     3181 cagaaacttc tgacctcgaa caaatggaat catgccggcg atctcggctt tcgcctagta
     3241 catgagcagg catctgtgcc tgagttggtg caagaaacag atctctggcg tcagtccgag
     3301 gggttccgag ccagggcatt tcagtttgtc gccgcgagag tctctccatg ctcttccgac
     3361 ggcagaaagg caatcgaact cgcggcgtca cgacccgagg cgattgtcga gttcatgctg
     3421 tcgagtgccg agcctttgtc cgagttgtct gtgcgtctga agtccattga tacgtcgtca
     3481 cagacattcg cgagcctgtt tctaaaaatg ctcgattaca tccggcgaac gggtgacgat
     3541 gagttcgccc gacagctcgg cgaagcgagc cctatcgcct ggtggcgtct gcatgccgct
     3601 ccgccgactt ctttcgccga gcgcgaagag cttcgccgac atctacagtt ggtcaacacc
     3661 gaactcgacc gctggcgtga gaccgacgaa cgccagcgcg ccctgacctc gcccgacgct
     3721 tggcagatca aggctacgaa gctgtttctc accgcgctcg acgaattccg cgaatcgaac
     3781 cccggcctcg tcgagtgggc tgtaaagcac atcgtcgacc tggcgacgtc cgcggatgcc
     3841 cgccaccgcc tgccgatctc caagaccaac agcagtctca aggccgtcgg ccgcagtaag
     3901 caggatggcc gcctgctctt caccctcgac gggaagtccc gcgaactccg cctctatcgc
     3961 ctctaccccg tccaacacca cgacgcctac gaacgcgacc tcagcaagcc ggaaatcagc
     4021 ggcgagctac tggtcaccgt ccctgacttg atggggtagg cgttgaaaag gatgtcatta
     4081 cgatccgccg cggcactgag ggcacaggcc tcgatcaaac cagacaagaa cgacgacata
     4141 cggtctgccg cattttttgc acacgctcaa tgggttcaac atctcatctc tcctttgcat
     4201 gacatgcaat acgttcactc acccaaatca gttcactgcg cccgataacc gagtgcgccg
     4261 cagtgtctca cgccgcgcgg gcgatggcgg tgatgaccga ggtggctttg acaccttcgc
     4321 ggttcttctt gcggtcgaac gagcggacgg cggtgaactg tac
//
LOCUS       DAORHD010000238         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21505_length_4361_cov_4.866930, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000238 DAORHD010000000
VERSION     DAORHD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21505_length_4361_cov_4.866930"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKO06_06375"
     CDS             <1..1249
                     /locus_tag="PKO06_06375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV69307.1"
                     /translation="GLPANLLVSTVWLGWLGLSWYAFRLDIILEVVPVLLLVPVQWAT
                     MRLIQQFYDLRRRNIDLARKVRELSLVNEVSQAVNFMGDLTKTLDTILSRAILALSAE
                     RGSILLLDERYENLVEESVLIGSSSRTDPTRSGLSDDLKKLFRTGEGIAGEVFTKGLP
                     RLIEHVPGTAEFAALDPDDHQDIRSLICVPLQVRNYAIGVMNVVNRAEGPFDQEDLQL
                     ALTMANQAAVVIEKARLFNLATIDGLTGLIVHRHFQSKLEEEFRRAKRYDKPLSLIMT
                     DIDHFKKFNDTWGHQTGDLVLREVAKTVRATVRDTDSPARYGGEEFAIMLPETDLEGA
                     VLFAERLRLRVEQMVVAGPKGPLQVTISLGVSAVPINPAETTLEMIKLADEALYEAKH
                     SGRNRVMGSAVREAGVPAEPQVS"
     gene            1350..3278
                     /locus_tag="PKO06_06380"
     CDS             1350..3278
                     /locus_tag="PKO06_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69308.1"
                     /translation="MNRMFQLLICLMVLGVAVVGCGGGGEASKPEPVAKPAEPAAAPV
                     DPLAREKEWGRCLARLAKASYNRGGLIYENLLVSARQFPEKTQAGLREVIREKSEGWD
                     YALLLLKEIPAKEDQELRDLVVKVVRQDGYSWGRNDAIALAGHLRLTDIAGEVLDYLK
                     TLKPNDTEGFPAARALLATAKPNGLRELVLSQLTQNVAPSGETLGAAAELLGTVDPGE
                     AQKLLPERLMKEDGVEAEYIGAARGLGIAGGEPGKRALLDFVKRFEERLDTHKNMYVR
                     KVGNAFYVAVAELSRFEGEEVWEYLLLYDSGYPYQLIAEQKRVESAITKIPSTYREKV
                     ARAWAFAPWIHNKKYRPKGSENKRRFFGERVLAAQLNSELKALLLKELAEYGDDPKYD
                     GNVSEVLLRLSEFAKAGDQEIKTLLRQWARPGKADTFTSRMAARQELQRTRDAVLLEY
                     LVGDNIWENASDWNRAREFDAVVVPGLSDAFLKFPHKPDSYGNVACDVVYKFLAAESR
                     ADVLAFLRQEIEKHLDSPGKTPAAHYLLALHSWKGTEVDDFYRSVIARYEKQGVEYTR
                     GYATNEVQAKREVLAAAYVALSTRGDPNAFRKVYAMKPEVYNETWKGVPVEYAAFLKE
                     DPGPVTIHWPTADALLPK"
     gene            complement(3304..>4361)
                     /locus_tag="PKO06_06385"
     CDS             complement(3304..>4361)
                     /locus_tag="PKO06_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV69309.1"
                     /translation="GPFVAVNCSAISENLFESELFGHEKGSFTGALRTHKGRFERAHG
                     GTLFLDEIGDIPTPFQAKLLRVLETGSIDRVGGEHPVQVDVRVIAATNRPLEEDARAN
                     RFRADLYYRISALQIHLPPLRERADDIPDLVRHFISQLNEKYQRRVAALTPEAVHLLQ
                     QYWWPGNVRELRNLMERLFAENQADVIGLRSLKDWYEERVQAVRHGVAYDPRVTSLPY
                     QQPIPLGMELQQSTSPVGVFNSPTGSSAPGTAGLTGYRPSSNSTASAHETLLRPNEEN
                     WVTDRAQPIQAASVNGTRPPLNGGSTPPVELTEEQVRQAVQASGGNMTQAARRLGVHK
                     ATLYRFLKAHNLTRDTL"
BASE COUNT          866 a         1185 c         1408 g          902 t
ORIGIN      
        1 cgggttaccc gccaatctcc tggtttcaac ggtctggctc gggtggctgg ggctttcctg
       61 gtatgccttt cggcttgata tcattctcga ggttgtcccc gttcttctgc ttgttccggt
      121 gcagtgggcg acgatgcggt tgattcagca gttttacgat ctgagacgcc gaaacatcga
      181 tctggcgcgc aaagtgcgcg aactctccct ggtcaatgag gtcagtcagg ccgtgaattt
      241 catgggcgac ctcacgaaaa ccctcgatac aattctttcg cgcgcgattc tggctctctc
      301 ggccgaacgc gggtcgattc tgctgctgga cgaacgctat gagaatctgg tcgaagagtc
      361 ggtgctgatc ggcagttcaa gccgcaccga cccgacccgc agcgggctca gcgatgattt
      421 gaaaaaactg ttccgcacgg gagaaggcat tgcgggagag gtgtttacca aaggcctgcc
      481 gcggttaatc gaacatgtac ccggaacagc tgaatttgcc gcgctcgacc ccgatgatca
      541 ccaggacatt cgttcgctga tctgcgtgcc gttgcaggta cgcaactatg cgatcggggt
      601 catgaatgtc gtcaatcgcg cggaagggcc gtttgatcag gaggatctgc aactggcgct
      661 gaccatggcg aaccaggccg cggtggtcat tgaaaaagcc cgcctgttca acctcgccac
      721 cattgatgga ctcaccgggc tgatcgtgca tcgccatttt cagtcgaaac tcgaagagga
      781 gttccggcga gccaagcgct atgacaagcc gttgtcgctg atcatgaccg acatcgatca
      841 tttcaagaaa ttcaatgaca cctggggaca tcagaccggt gacctcgttc tgcgtgaagt
      901 ggccaagacc gttcgtgcga ccgttcgtga caccgatagc ccggcacgct acggcggcga
      961 ggagtttgcc atcatgctgc ccgaaaccga tctggaaggt gccgtcctgt tcgcggaacg
     1021 tctgcgactg cgggtcgagc agatggtcgt ggctggtccg aaaggtccgc tccaggtgac
     1081 catcagtctt ggggtgtctg cggtcccaat caatccggct gaaacgacgc tcgagatgat
     1141 caaactcgcc gacgaggcct tgtacgaagc caaacactcc gggcgcaacc gggtcatggg
     1201 cagcgcggtg cgcgaagccg gtgtccccgc ggaaccgcag gtttcctgag agtctgcttg
     1261 tctgctgaga ctgactgtct cgctgttcta ccgccgctcg tctctgtcga ggcggtgaaa
     1321 agaccattcg ttttcgaggg aggatcgcga tgaatcgtat gttccagctt ctgatctgcc
     1381 tgatggttct gggtgttgcg gttgtcggct gtggcggtgg gggcgaggcg tcgaaacccg
     1441 aaccggtcgc gaaaccggca gagccagctg cggcaccggt ggatccgctg gcccgcgaaa
     1501 aggagtgggg gcgctgtctc gcgcgtctcg cgaaagcttc gtacaatcgc ggcggcttga
     1561 tctatgagaa tcttctggtg tcggcgcgcc agtttccgga aaagacgcag gccggtctgc
     1621 gggaagtcat tcgcgaaaaa tcggaaggct gggattacgc gttgctgctc ttgaaggaga
     1681 ttcccgcgaa agaagaccag gaactgcgcg acctcgtggt gaaagtggtt cgtcaggacg
     1741 gttattcctg gggcaggaac gacgcgatcg cactggctgg tcacctgcgg ctgaccgaca
     1801 ttgccggtga agtgctcgac tatctcaaga ctctcaaacc caatgacacg gaagggtttc
     1861 ccgctgcccg tgctcttctg gccaccgcca agccgaacgg gttgcgcgag ctggtgctgt
     1921 cgcagttgac tcagaatgtg gctccgagcg gtgagaccct tggggcggcc gccgagctgc
     1981 tggggacggt cgatccgggc gaagcccaga aactgcttcc tgagcgactc atgaaggaag
     2041 acggagtcga ggcggaatac attggtgccg cccggggtct gggcattgcc ggcggagagc
     2101 cgggcaaacg agcgttgctg gacttcgtga agcgctttga agagcggctg gatacccaca
     2161 agaacatgta tgttcgaaaa gtcggcaacg cgttctatgt ggccgtggcg gaactgagtc
     2221 gcttcgaagg cgaagaggta tgggagtatc tgcttctcta tgattccggg tatccgtacc
     2281 agttgattgc ggaacagaaa cgcgtggaaa gtgcgattac gaagataccg tcgacctatc
     2341 gtgagaaggt ggcgcgggcg tgggctttcg cgccttggat ccacaacaaa aaataccgac
     2401 cgaaaggttc tgaaaacaaa cgacgcttct ttggtgaacg agtgctggcg gcgcagttga
     2461 acagtgagtt gaaggcactg ctgctgaagg agctggctga gtatggcgat gacccgaagt
     2521 atgacggcaa cgtctctgaa gtcctgctgc gtttgtccga gtttgccaag gcgggcgatc
     2581 aggagataaa gaccctgttg cgacagtggg cgcgaccggg caaggccgac accttcacat
     2641 cgcgcatggc ggcgagacag gagctgcagc gcaccaggga cgcggttctg ctggagtatc
     2701 tggtcggtga caacatctgg gagaatgcct cggactggaa tcgggcgcgg gagtttgacg
     2761 cggtcgtcgt tcctggcctg agcgacgcct ttctcaagtt tccgcataaa cccgactcgt
     2821 acggaaacgt cgcctgtgat gtcgtgtaca agtttctggc cgcggaatca cgagccgacg
     2881 tgctggcctt tctgcgccag gagatcgaaa agcatctcga cagtccgggc aagactcccg
     2941 cggcgcacta tctcttggct ttgcattcct ggaaggggac cgaagtggat gacttctatc
     3001 gcagcgtgat cgcccgctat gaaaaacagg gagtggaata cacgcggggc tacgcgacca
     3061 atgaggtgca ggcgaaacgg gaggtgctgg ccgcggccta cgtggcgctg tctacgcgtg
     3121 gtgatccgaa cgcctttcgt aaggtctatg ccatgaagcc cgaggtctat aatgagacct
     3181 ggaagggtgt accggtggaa tatgcggcgt ttctgaaaga agacccgggc ccggttacca
     3241 tccactggcc gacggccgat gccctgttgc cgaagtaagg cggggcgctg agcgcaggtt
     3301 cgattacagc gtgtcgcgcg tgaggttatg cgctttcagg aagcgataga gcgtggcctt
     3361 gtgcacgccg aggcggcgtg ccgcctgggt catgttgccg cccgacgctt gaaccgcctg
     3421 ccgaacctgc tcttccgtga gctcgacggg cggggttgag ccgccattca gcggagggcg
     3481 cgtgccgttg accgacgccg cctgtatggg ctgcgcgcga tcggttaccc agttctcttc
     3541 attcggtcgt agaagggttt catgtgccga tgctgtgctg ttcgagctcg gacgataccc
     3601 ggtgagtccg gcggtgcccg gtgctgagga acccgtggga gagttgaaca ccccgacggg
     3661 agaagtcgac tgctgcagct ccatgccgag tggaatcggc tgctgatagg ggagcgaggt
     3721 gactcgtggg tcgtaggcca cgccgtgacg aaccgcctgt actcgctctt cgtaccagtc
     3781 cttgagtgat ctcaagccga tgacatcagc ctggttctcg gcgaacaatc gttccatgag
     3841 gttgcgcaat tcgcgcacgt ttccgggcca ccagtattgc tgcagcagat gaacggcttc
     3901 gggagtgagt gcggcgacgc gacgctggta cttttcgttc aactgactga taaaatgtcg
     3961 caccagatcg ggaatgtcat cagcgcgttc acgcagcggc ggcagatgaa tctgcagagc
     4021 gctgatgcga tagtacaggt cggcacggaa tcgattcgcg cgcgcatctt cctcgagcgg
     4081 tcgattggtg gcggcgatca cacgaacgtc aacttgtacc ggatgctccc cgccgacacg
     4141 gtcgatgctg ccggtttcga gcactcgcag cagtttcgcc tgaaacgggg tcggaatgtc
     4201 gccgatctcg tcgaggaaca gtgtgccgcc gtgcgcgcgc tcgaagcgac ctttatgcgt
     4261 gcgcagtgcg ccggtaaacg agcctttctc atgaccgaac agttcgctct caaagagatt
     4321 ctctgaaatg gcgctgcagt tcaccgcgac gaacggtccg g
//
LOCUS       DAORHD010000239         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21506_length_4361_cov_4.333720, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000239 DAORHD010000000
VERSION     DAORHD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21506_length_4361_cov_4.333720"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2598)
                     /locus_tag="PKO06_06390"
     CDS             complement(<1..2598)
                     /locus_tag="PKO06_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV69310.1"
                     /translation="MPAKKTPMKKTVVTPKKAVKAAPAKPAQKAVAKPAAKKPAPTAK
                     KASKPAAKLEKPAKRPVVKPAAKAVPAKKIVPVAKPAKPQVKPVAVKPMPKPAPKPEP
                     KPVAKAKPVEPKPQPKPQKPTHTTAATASVIPESPLTFPRPKKFQVVAKSKHKLDGIG
                     LVCGIQKYVMDLDLQNPVYVKIMTSPHAHARIKKIDTAEAFKIPGVVAIYTWKDVPRI
                     PHTTAGQGFPEPSPYDTFILDNKVRFVGDRVAIIAADTPEAAQEACRRIKVEYDILPA
                     VFDPEKARDPGAPVIHDEPEAYMPIPTFFDPKHNHCAHMEARVGDVEKGYKDADYVLE
                     RKYYPHYAQHCPIEPHCCIGYLDENDRLVLRTSTQVPFHARRITAQALDIPLKKIRVI
                     KPRIGGGFGTKQEVLLEDVVALVTLRTRRPAILELTRPEEFVSSRTRHPMVVTIKAGV
                     KKNGEITALSMKVISNTGAYGSHALTVMCNCGSKVLPLYKAKNVEFIGDTVYTNLPVG
                     GAYRGYGATQAAYGVEALMDECAKAIGMDALEFRKRNHIREGDSSPIFKALGEGREGV
                     DMAIKSCGLPECIEWGAKEIDWKNKRNRPGKGVKRRGIGMAILMQGSSIPEIDMGAVS
                     IKMNDDGSFNMLLGATDLGTGSDTILAQIAAETLGCRHDQLLVYSSDTDMTPFDVGAY
                     ASSTTYLSGGAVINTANKVREQILAVASIMPKMQGATDLEAEDGWVYSKSDTDLKVSF
                     AEIATFSLYEKRQFQIAAVGSHISHASPPPFSAHFAEVEVDTETGEVKLVKYAASVDC
                     GTPINPQLAEGQLQGAVVNGISFALTEEYIFDSQGRMLNPNFRYYKIYTANDMPDLKT
                     HLAITYEPTG"
     gene            complement(2618..3085)
                     /locus_tag="PKO06_06395"
     CDS             complement(2618..3085)
                     /locus_tag="PKO06_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV69311.1"
                     /translation="MKLQLIINGERKSFEIAPQELLLDVLRRNGYKSVKKGCGEGACG
                     ACAVLIDGRVRNSCTVFAAHCEGSEVLTTEGLGTPDHPHVLQRSFVDHGATQCGFCNP
                     GSLLAAKALLDRNAEPTEAEVKEALDGNLCRCTGYVKRIEAVLDAAKTLKKHQ"
     gene            complement(3096..3956)
                     /locus_tag="PKO06_06400"
     CDS             complement(3096..3956)
                     /locus_tag="PKO06_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNV69312.1"
                     /translation="MLRHLTQFYRPQTVQEACTLLEDKTRKNVPLAGGTYLTQIDDAT
                     IDGLVDLKGLQLSSIRADSGGFRIGAMTPVFDVATSTVLTGPAGALLREAAKRIGSTP
                     LRHSITVGGNLVGVFPWSDLPPALLVLEAEVTLANAHRKRTIPLTTLYATRPNDVIER
                     GEIMTEIHVPAMPTGSKNGTAFIKMAKTANDYAIITVATRLTFNGGIISAARIAINAP
                     TRKPTRCRDAEDSLKGKQITPELLAEAGVRAISGIEFTPDFRASKEYRMDVLPVVVRR
                     ALEQAAKANA"
     gene            4143..>4361
                     /locus_tag="PKO06_06405"
     CDS             4143..>4361
                     /locus_tag="PKO06_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69313.1"
                     /translation="MLKKAMSAALVGSMLTLAPAPTEAKNILKDLLKVYQTYQEVNMM
                     LWLTGDAKAEKRFGEEVKWFVNLTNKKEK"
BASE COUNT          732 a         1260 c         1422 g          947 t
ORIGIN      
        1 gccggtcggc tcgtaggtga tggccagatg cgtcttcagg tcgggcatat cgttcgccgt
       61 gtagatcttg tagtagcgga agttcggatt caacatgcgt ccctgactat cgaagatgta
      121 ctcttcggtc agcgcgaaac tgatgccgtt caccaccgcg ccctgcagct gcccttcggc
      181 gagctggggg ttgatcggcg taccgcagtc gaccgacgcg gcatacttca ccagtttcac
      241 ttcgcccgtc tcggtgtcga cttcgacttc ggcgaagtgg gccgagaacg gcggcggcga
      301 tgcgtgcgag atgtgactgc cgacggcggc gatctggaac tgccgcttct cgtagagcga
      361 gaaggtcgcg atctcggcga agctcacctt gagatcggtg tcggacttcg agtagaccca
      421 gccgtcctcg gcttcgaggt cggtcgcgcc ctgcatcttc ggcatgatcg aggcgaccgc
      481 gagaatctgc tcgcgcacct tgttcgccgt gttgatgacc gcgccgcccg acagatacgt
      541 cgtcgaactg gcatacgcgc cgacgtcgaa cggagtcatg tcggtatcgg aactatagac
      601 cagcagctga tcgtggcggc aacccagcgt ttcggcggcg atctgggcca gaatggtgtc
      661 cgagccggtg ccgaggtcgg tagcgcccag cagcatgttg aacgacccgt catcattcat
      721 tttgatcgag acggcgccca tgtcgatctc gggaatcgag cttccctgca tgagaatcgc
      781 cataccgatg ccgcggcgct tgacgccctt gccgggccga ttgcgcttgt tcttccagtc
      841 gatttctttt gcgccccatt cgatgcactc gggcagaccg cacgacttga tcgccatgtc
      901 gacgccttcg cgcccttcgc ccagagcctt gaagatcggg gaagaatcgc cttcgcgaat
      961 gtggttgcgc ttgcggaact ccagagcgtc catgccgatc gccttggcgc actcgtccat
     1021 cagtgcctcg acgccgtagg ccgcctgcgt ggcgccgtac ccgcgatacg cgccgccgac
     1081 cggcaggttg gtatacacgg tgtcaccgat gaactcgacg ttcttcgcct tgtacagagg
     1141 cagaaccttc gagccgcagt tgcacatgac ggtcagcgcg tgactgccgt aagcgccggt
     1201 gttgctgatg accttcatcg acagcgccgt gatctcgccg ttcttcttca cgccggcctt
     1261 gatggtgacc accatcgggt ggcgggtgcg cgatgagacg aactcttcgg ggcgggtcag
     1321 ttcgagaatc gccggtcgcc gcgtgcgcag agtcaccaga gcgaccacgt cttccaggag
     1381 tacctcttgc ttggtgccga aaccgccgcc gatacggggt ttgatgaccc gaatcttctt
     1441 cagcggaatg tcgagcgctt gcgcggtgat gcggcgcgca tggaacggca cctgcgtcga
     1501 ggtgcgcaga accaggcggt cgttctcgtc gagatacccg atacagcagt gcggttcgat
     1561 cgggcagtgc tgggcgtagt gcgggtaata cttgcgttcg agcacgtagt cggcgtcttt
     1621 gtatcccttt tcgacgtcgc cgacgcgggc ttccatgtgc gcgcagtggt tgtgcttcgg
     1681 gtcgaagaac gtcggaatcg gcatgtaggc ttcgggttcg tcatgaatca ccggagcgcc
     1741 tggatcgcgg gctttctcgg gatcgaagac cgcgggcaga atgtcatact cgaccttgat
     1801 gcggcggcag gcttcctgcg cggcttcggg cgtgtcggcg gcgatgatgg ccacgcggtc
     1861 gcccacgaag cgcaccttgt tgtcgagaat gaacgtgtca tacggcgacg gctcgggaaa
     1921 gccctgaccg gcggtggtat gcggaatgcg cggcacgtcc ttccaggtgt agatggcaac
     1981 gacgccggga atcttgaagg cttcggcggt gtcgatcttc ttgatgcggg cgtgggcatg
     2041 cgggctggtc atgatcttga catagaccgg gttctgcagg tccagatcca tgacgtactt
     2101 ctgaataccg cacaccaggc cgatgccgtc gagcttgtgc ttggacttgg cgacgacctg
     2161 gaacttcttc gggcgcggaa aggtcagcgg tgactcggga atgacgctgg cggtagccgc
     2221 cgtcgtgtgc gtcggctttt gcggcttggg ttgcggcttc ggctcgaccg gctttgcttt
     2281 tgcgaccggt ttgggttccg gcttcggagc gggtttgggc atcggtttta cggcaaccgg
     2341 cttcacctga ggcttggcgg gcttggcaac cggtacgatt ttcttcgccg gaactgcctt
     2401 cgcggccggc ttcacgaccg gtctcttcgc gggcttttcg agcttggcgg cgggcttgct
     2461 cgccttcttg gcggtcgggg cgggtttctt cgccgctggt ttcgcgaccg ctttctgagc
     2521 gggcttggca ggagcggcct tcacggcttt cttcggggtg acaacggttt tcttcatcgg
     2581 agtttttttg gccggcatgt gtgttccctt ctcctgctta ctggtgtttc ttcaaggtct
     2641 tggcggcatc gagcacggct tcgatgcgct tgacataccc ggtgcagcgg cagaggttcc
     2701 cgtcgagggc ttccttcacc tcggcttcgg tcggttcggc gttgcggtcg agcagagcct
     2761 tggcggccag cagcgacccg gggttgcaga acccgcactg ggtggcgccg tgatcgacga
     2821 acgatcgctg cagcacgtgc ggatggtcgg gcgtgcccag gccttccgtg gtcagcactt
     2881 cagagccttc gcagtgcgcg gcaaacaccg tgcacgagtt gcgcacgcgg ccgtcgatga
     2941 gcacggcaca agcgccgcag gctccttcgc cgcacccctt cttgacactt ttgtacccgt
     3001 tgcgccgcag cacatcgagc agcagttcct gcggagcgat ctcgaacgac ttgcgttctc
     3061 cgttgatgat cagttgcagt ttcattcctg gctccttatg cattggcttt ggccgcctgt
     3121 tcgagggcgc gccgcacgac gacgggaaga acgtccatcc ggtattcttt cgaagcccgg
     3181 aagtcgggag tgaactcgat gccgctgatg gctcgcacgc cggcttccgc cagcagttcg
     3241 ggcgtgatct gcttgccctt cagcgagtct tcggcgtcgc gacagcgggt cggcttgcgg
     3301 gtcggcgcgt tgatggcgat acgagccgcg gaaatgatgc cgccgttgaa ggtcagtcgc
     3361 gtggcgacgg tgatgatcgc atagtcgtta gcggtcttgg ccatcttgat gaaggccgtg
     3421 ccgttcttcg aaccggtcgg catggccgga acatgaatct cggtcatgat ctcgccgcgt
     3481 tcgatgacat cgttcgggcg agtggcgtac agggtggtca ggggaatggt gcgcttgcga
     3541 tgagcgttcg ccagggtaac ttctgcttcg agaaccagca gcgccggggg caggtcagac
     3601 cacggaaaca cgccgaccag gttgccaccg acggtgatgc tgtgtcgcag gggcgtcgag
     3661 ccgatgcgct ttgcggcctc gcgcagcaga gcgcccgcgg gccccgtcag cacggttgac
     3721 gtggcgacat cgaacaccgg ggtcatggcc ccgatgcgga acccgccgct gtcggcgcga
     3781 atcgaagaga gctgcaggcc cttcagatcg accagtccgt cgatggtcgc gtcgtcgatc
     3841 tgggtcagat acgtgccgcc cgccagaggc acgttcttcc gggttttgtc ttccagaagg
     3901 gtacaagcct cttggaccgt ctgggggcga taaaattgag tgagatgtct gagcatgggt
     3961 tctcccgtac gaagatatct gtgtgatcga atgacaggat tatacctata cccgtgtcga
     4021 ctcgccaaca acaaaccaca cctttgctct cgtaaagtta gcaatccgcg agagtggctt
     4081 ccccgttttc gtcggggtgc gttataatgc gctgttctcg aagcccacga aggaggggag
     4141 cgatgttgaa aaaggcgatg agcgcggctc tggtcggcag catgctgact ctggcgcccg
     4201 cgccgactga agcgaagaat attttgaaag atctcctgaa ggtctaccag acgtatcagg
     4261 aagtgaacat gatgctctgg ctcaccggag atgccaaggc cgagaagcgg ttcggcgaag
     4321 aagtgaaatg gttcgtgaac ctgaccaaca agaaagaaaa a
//
LOCUS       DAORHD010000240         4357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21541_length_4357_cov_3.293352, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000240 DAORHD010000000
VERSION     DAORHD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21541_length_4357_cov_3.293352"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKO06_06410"
     CDS             complement(<1..468)
                     /locus_tag="PKO06_06410"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNV69314.1"
                     /translation="MTSSPGSSRRLVAVLGPTAGGKSALALLLARRLGCEIINCDSRQ
                     IYRGMDIGTAKPTPNERREIPHHLFDLVEPTQEYSAGAYSRAARRCIEDLWRLGRRPL
                     VVGGTGFYFQALMDGVPSAPADTGLRERILERLHDEGLQVLVDELRRLDPVGAA"
     gene            complement(473..2404)
                     /gene="mutL"
                     /locus_tag="PKO06_06415"
     CDS             complement(473..2404)
                     /gene="mutL"
                     /locus_tag="PKO06_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNV69315.1"
                     /translation="MADQLIQTLSSEVVNKIAAGEVAERPSAVVKELIENSIDASARR
                     IEIEIKDGGQKLIEVADDGVGMSAEDAVAAFGRHATSKIRSDEDLERLSTLGFRGEAL
                     ASIAAVSRTTLTTRREGAETGTQVVIEGGSVRAVEAVAHARGTRLQVKNLFYNVPARR
                     KFLKSQQAEMSAIADIVCHYILGYPELAFRLTRNNVPLVQSPGTGDLLDAVLAVHAPE
                     LVKSLLPLKTPASAQEGTVSIRGFISPPNAAKPAARYQTILINRRSVKSKLIAAAISK
                     ALSPFFPRGKYPVLILDLRLPPESVDVNVHPQKTEVRFVDERWVFGLVHEALQISLSG
                     LKMVQSLPPEDVERVKNLSLWPESAREEVLPAQNADEGRRSEGLPERPIAKNRPSVYD
                     RLARSAEPETEAAPSTPLSSEKTEPASSAARLYELRETSERLGPESKPIAQAGFEPVQ
                     VLQEPRILSQCLNAYLLGEDRDGLFLVDQHAAHERVLFDAFVEAYRDERIHSQPLLFP
                     LPLKLLPSERSLVLEKADELKNLGFSIQQEEDGQFYAVAIPICDGKPLEASEIQDLLG
                     RLFDDWENRSLQERKLDLFKMMACKAAVKAGDPMREPEMRSLFEQLLKTPNPFTCPHG
                     RPTVIRLSRRQIETGFLRT"
     gene            complement(2427..3437)
                     /gene="tsaD"
                     /locus_tag="PKO06_06420"
     CDS             complement(2427..3437)
                     /gene="tsaD"
                     /locus_tag="PKO06_06420"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNV69316.1"
                     /translation="MLTLGIESSCDDTSLAIIEDGVRVRASLVSSQIDVHRRFGGVVP
                     EVASRKHLEAIHPLLQQVLQQADLSLDEIGQIAVTTGPGLLGALLVGVSVGKALAWLE
                     KKPLIPVNHLEAHLYAAFLAQNRAPEYPFLGLIVSGGHSTLALVEGARRIRTLGRTVD
                     DAPGEFFDKIARHLQLGYPGGPVVQKAGEGGDPSRYPLPRPMVGRGYDFSFSGLKTAV
                     LQRAKLEGDALVVRDLCAAMQQAVAGSLTEKVARALEETRVPRLVVAGGVAANAVLRE
                     RMAALATQQRVELLIPGLNLCTDNAAMIGAAGYWTAEWWQTDTLLVNAQADWELGAPL
                     SD"
     gene            complement(3431..4084)
                     /locus_tag="PKO06_06425"
     CDS             complement(3431..4084)
                     /locus_tag="PKO06_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69317.1"
                     /translation="MNPVSSYLFFDASFPTAMGQIGTPEKYQSFSWETRQDLAAHQTR
                     YVDRMLSSCDTTFEQIARIGVGVGPGSLTGLRMTVGFFRTLAMYGGKPLLGVSLFDWA
                     AKTLERQGRSGTFRLALPGPLKGQFVTEVSIPGGGPIAQAQIRFFSAEAFRELPEVMV
                     IRQHVPNCPSLELDPAALDQIMRTFVPATGEYLLRVVPFYVIPSQAEINWEKRAQSC"
     gene            complement(4081..>4357)
                     /locus_tag="PKO06_06430"
     CDS             complement(4081..>4357)
                     /locus_tag="PKO06_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNV69318.1"
                     /translation="IGLKANVNSPSYTVLNVYREGGRALYHLDLYRLECFEEIYDVGL
                     FEAIDAGFPCVVEWPERVPELSRLPHLLVTLEPQTNESRRLCWKWNS"
BASE COUNT          972 a         1344 c         1178 g          863 t
ORIGIN      
        1 ggcagcaccc acgggatcaa ggcggcgcaa ctcatcgacc agtacctgaa gcccttcatc
       61 atgcaatcgc tccaggattc gctcacgcaa tcccgtgtcg gcaggagcgc tcggcactcc
      121 gtccattaat gcttgaaaat agaatccggt gccgccaacg accagcggtc gacgcccaag
      181 ccgccagagg tcttcgatac aacggcgcgc cgcacgcgaa taagcgccgg cactgtattc
      241 ctgcgttggt tctaccagat cgaacagatg atgcggaatt tcccgccgct cgttcggtgt
      301 cggcttggca gtgccgatgt ccattccccg atagatctgg cgcgaatcgc agttgatgat
      361 ctcacaaccc aaccgcctgg ccagcagtaa agccagggct gacttgcctc cggccgttgg
      421 gccaagaacc gcaaccaacc ggcgagagga accaggggat gaggtcatcg tctcaggtgc
      481 gtaaaaagcc agtttcgatc tgtcgccgac tcaggcgaat caccgtcggt cgaccgtggg
      541 ggcaggtaaa cgggttggga gtcttgagta actgctcaaa caacgaccgc atctcgggtt
      601 cacgcatggg gtctccggct ttcactgcgg ccttgcaagc catcatcttg aacagatcca
      661 gtttccgctc ctgaagcgaa cggttttccc aatcatcgaa caagcgtccg aggaggtcct
      721 gaatctcact ggcttccaaa ggtttgccat cacagatcgg aatggcaaca gcatagaact
      781 gtccgtcttc ctcctgttga atcgagaaac ccagattctt gagttcgtcc gctttctcga
      841 ggacgagact gcgctcagaa ggcagcagtt tcagcggcag aggaaacaac aacggctgcg
      901 agtggatccg ctcatcgcga tacgcctcga caaatgcgtc aaagagaacc cgttcatgcg
      961 cagcatgctg gtcaaccaga aacaggccat cacgatcttc gccgagcaga tacgcattga
     1021 ggcattgcga tagaattctc ggctcctgaa gcacctgcac cggctcgaaa cccgcctggg
     1081 ctatcggctt ggattcaggc cccaggcgtt cactcgtttc tctcaactcg tacagtctgg
     1141 ccgcagaaga agcaggttcg gttttttcag atgacagggg tgtcgacgga gcggcctcag
     1201 tctctggttc tgctgatcgc gccagacggt cgtagaccga ggggcggttt ttcgcgatcg
     1261 gtcgttcggg aagtccctca ctccgccgac cttcatcggc attctgagcg gggagaactt
     1321 cttctcgcgc cgactcaggc cacagcgaga gattcttcac ccgttccacg tcttcggggg
     1381 gaagggattg caccattttc aggcctgata acgagatctg cagtgcctcg tgcaccagac
     1441 caaacaccca gcgctcatcg acaaatcgca cttcggtctt ctgcggatgc acgttcacat
     1501 caacggattc aggcggcagt cgtagatcaa gaatcaggac cgggtatttg ccgcgaggga
     1561 aaaacggcga caatgccttg ctgatcgccg cggcgatgag tttggacttg accgaccggc
     1621 ggttgatgag aatcgtctgg tagcgggcag ccggcttggc agcattcggt ggagagatga
     1681 acccgcgaat gctcactgtg ccttcctggg cactggcagg cgttttcaga ggcaacagac
     1741 tcttgaccaa ctcgggtgca tgaacggcaa gaacggcatc gagcagatct ccagtccccg
     1801 gagattgtac cagtggcaca ttgtttcgag ttaatctgaa tgccagttcc gggtaaccaa
     1861 gaatatagtg acacacgatg tcggcaatgg ccgacatttc agcctgctga gacttgagaa
     1921 actttcggcg cgccggcacg ttgtagaaga ggtttttcac ctgaaggcgg gtgcctcgcg
     1981 cgtgcgcgac tgcttcgacc gcccggacac tgcccccttc gatgacaacc tgggttccgg
     2041 tttcagcccc ttcgcgacga gtggttaacg tcgtgcgtga gaccgcggcg atactggcca
     2101 gcgcttcacc ccgaaacccc agggtgctca gcctctcgag gtcttcatca gagcgaatct
     2161 tgctggtggc atgacgaccg aaagccgcga cagcatcttc cgcgctcatg cccaccccat
     2221 catcggcgac ttcgatcagt ttctgaccgc catccttgat ctcgatctcg atacgccgag
     2281 ccgaggcatc gatactgttc tcgatcaact ctttcacgac agcggaaggt cgttcggcaa
     2341 cttcgcccgc cgcgattttg ttcaccacct cagaagaaag cgtttgaatc agctggtccg
     2401 ccacgttcgt ccctcgctct tttgtcctag tcgctcaacg gagcaccaag ctcccaatct
     2461 gcctgagcat tcaccaacaa agtatccgtc tgccaccact ccgcggtcca gtaacccgcg
     2521 gcgccgatca tcgcggcgtt gtccgtgcag agattcaagc ccggaatcag cagctcaacc
     2581 cgttgttgcg ttgccagggc ggccatgcgt tcgcggagca cggcatttgc cgccacacca
     2641 cctgccacca ccagcctcgg cacccgcgtt tcctcgagcg cccgagcgac cttctcggtc
     2701 aacgaaccgg ccaccgcctg ctgcatggcg gcgcacaggt cacgtacgac caacgcgtct
     2761 ccctcgagtt ttgcccgctg caacacggcc gttttcaaac cactgaaact gaaatcatag
     2821 ccccgcccca ccataggccg cggcagtgga taacgggagg ggtcaccgcc ctcaccggct
     2881 ttctggacaa ccgggccgcc tggatagccc agctgcagat ggcgagcgat tttgtcgaaa
     2941 aactcacccg gggcgtcatc gaccgttcgc cccagagtcc tgatgcggcg tgccccttcc
     3001 accagcgcaa gcgtcgagtg ccccccagat acgatcaggc cgagaaatgg gtattcaggc
     3061 gcacgattct gggccagaaa agccgcatac agatgagctt caagatgatt gaccggaatc
     3121 aatggcttct tttccagcca ggcaagagcc ttgccgacgg aaaccccgac caacagagcc
     3181 cccaacaagc ccggaccggt tgtgaccgca atttgtccga tctcgtcgag actgagatcc
     3241 gcttgctgca agacttgctg caacaaagga tgaatggctt caagatgttt gcgcgaggcc
     3301 acctcgggca ccactccccc aaatcgtcga tgtacgtcga tctgcgacga gacgaggctg
     3361 gcgcgcacgc gcacgccatc ttcaatgatc gccagtgacg tatcgtcgca actgctctca
     3421 atgccgagcg tcaacatgac tgcgccctct tttcccagtt gatttcggcc tgagagggaa
     3481 tgacataaaa tggcaccacc cgaagcaggt attctccggt cgccggaaca aatgttctca
     3541 ttatttgatc taaagcagcc gggtcaagtt caagactcgg acaattcggc acatgctggc
     3601 gaatcaccat cacttctggc agctcacgga aggcctcggc cgagaaaaac ctgatctgcg
     3661 cctgtgcaat cggaccacca ccgggaatcg aaacctcggt gacgaattgt cccttcagag
     3721 gcccaggaag agccaggcgg aaagttccgc tgcgcccttg ccgttcgagt gttttcgccg
     3781 cccagtcaaa cagactgact cccagcaggg gcttgccgcc atacatggcc agcgtacgaa
     3841 aaaagccgac cgtcatccgt aagcccgtca ggctccccgg accgacgcca acgccgatac
     3901 gcgcaatctg ttcgaacgtc gtgtcgcagc tcgacaacat gcggtcaaca tatcgagtct
     3961 gatgtgccgc gagatcctga cgcgtttccc aggaaaacga ctggtacttt tccggagtgc
     4021 caatctggcc catcgcagtc gggaatgaag catcaaaaaa gagataggat gaaacaggat
     4081 tcatgagttc cacttccagc agaggcggcg agattcattg gtttgcggct cgagcgtcac
     4141 cagcaggtga ggcaagcgag acagttctgg aacacgctcc ggccactcaa cgacgcaggg
     4201 gaacccggca tcgatggcct caaacaggcc gacatcgtag atttcttcaa aacattccaa
     4261 ccggtacaaa tcgagatgat acagggctcg ccccccctct cgatagacgt tcaacacggt
     4321 atacgaaggg ctgttgacgt tcgccttgag accgatt
//
LOCUS       DAORHD010000241         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21580_length_4353_cov_4.174267, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000241 DAORHD010000000
VERSION     DAORHD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21580_length_4353_cov_4.174267"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2440)
                     /locus_tag="PKO06_06435"
     CDS             complement(<1..2440)
                     /locus_tag="PKO06_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69319.1"
                     /translation="MAQCRSCGSKDARGIFCGVCGARLEPASAAATPKSSPSPVPAPK
                     ISVSPPPAADPPVTSTREASRLDQLTAELKANPGSPAAYLNLGEALFHVGKFDRAYST
                     FRAARAIAPEDPAILRMGARILDALGRRDEAIDLWDRCLKLSETDIEAGLQVARLLYE
                     SGRRREALARLQSWRRQSEQRPELLLRVAEIQLAVGNPADALEDISQYRKLAGNSREA
                     YLLQGRAMMAQNFADGAIGLYKEALTALPDDPDLRWGLGKALLAANERGQAMLEFERA
                     SKSAPDRVEILFELGRLSGEAGMEDRAEEVFQRIREANVRSGEIFLDLGRYYLSRRRI
                     DVALRELQRARELSPHHPEIIRTFAEALESKELWPKALSEYEAFLEGAPGTAWALQGL
                     IRCAGKLEAHEVTAKAQKAFIDAGHASPDAWCDLGETLIRLARFPDAEKAFEAAARLD
                     PTHVRAYQAPELIRIEKARAEGRKLVDQARDAIQKRFLLTAIERLERALALVPREHGW
                     MRLLADVYLRIGDLGRGSKLLTQVRAVMPNDYQVGYQLARTYEFEDKSQLAQELLSSV
                     LKDRPDEIDGHLMLLRLKRSQVQGDRFEKDMLASLLRTTRQDLAPVSKQAPVQQIVEG
                     YVHYLFGMGTKMQTESLKQAEELFEEALYRNNESVPAHRGLCLIYRARGDWRKATLHM
                     QELVKTSSDPSALYALARLHENFQHFEEARKCYTSLRSLFPENGLYRRMQILMAAREV
                     EVGSKNPLMDFINSCQETLREDGNHSWVLYDLAWAQTLVARQSPQRDEWAKRALLSWN
                     KAASHPDANEWARWG"
     gene            complement(2443..4218)
                     /gene="argS"
                     /locus_tag="PKO06_06440"
     CDS             complement(2443..4218)
                     /gene="argS"
                     /locus_tag="PKO06_06440"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV69320.1"
                     /translation="MKDTLSPVLQKALFPLFRELNIENPEAFIELQRPPEAKFGDFSL
                     NTAMRLAKPAKQPPQKIAQRLVELLKPIEEWFGKVEVVGPGFVNLFCTDASIEKALTS
                     MACDQRLGVPATGNGQIVVIDYSSVNIAKQMHVGHLRSTIIGDVLARVLEALGYTVIR
                     QNHLGDWGVPIAMVIWKTAPILRDLEQRGLDPAQELSLSRLEEIYRQVTQECASDPAK
                     AEECRKVLVSLQAGDPQLVKDWETITRISMSEVYRIYRLLNVTLTEKDERGESFYRDR
                     LAQTVQVIRESGKLEESQGAQCVFLQQFKTKDGSPLPIIVQKSDGGYNYETFDLAAVR
                     YRIADLHADRVIYVTDARQALHFSQFFAVADTCGWTNKNSKRIPLEHVTFGSVMGEDN
                     KPLKTRSGENVKLSDLLAEAVERSLNVVREKNGDLSPERQQAVAEAVGIGAVKYADLS
                     NNRNNDYVFSWDRMLALNGNTAPYLQYAHARICSIFRKGKIDETAVKNEFRLAQPAER
                     TLAMKLLQFPEVVSAVTVDLRPHVLCTYLFELAVAFSTFYDQCPVLTASEGAERNSRL
                     GLCGLCRRVLARGLDLLGIAAPQEM"
BASE COUNT          849 a         1264 c         1325 g          915 t
ORIGIN      
        1 gcccccaacg ggcccactcg ttggcgtcag ggtgtgaagc cgccttgttc cagctcagga
       61 gcgcgcgttt cgcccactca tcgcgttgag gcgactgtcg ggctaccagt gtctgcgccc
      121 aggccagatc gtacaggacc caggagtggt tgccatcttc acgcaaagtt tcctggcagg
      181 aattgatgaa atccatgagt ggattctttg agccgacctc gacttcacgg gcggccatca
      241 gaatctgcat gcggcgatag agaccgtttt ccgggaatag actgcgcagg ctcgtgtagc
      301 acttgcgggc ttcctcaaaa tgctgaaagt tctcatgcaa ccgcgccaga gcgtagagtg
      361 ccgatgggtc ggaactggtt ttcacgagtt cctgcatgtg cagagtggct ttgcgccaat
      421 caccgcgagc gcgatagatc agacacagcc cccgatgggc cgggacgctt tcattattgc
      481 gatacagggc ttcctcgaac aattcctcgg cctgcttcag tgattccgtc tgcatcttgg
      541 tgcccatgcc gaagaggtag tgaacatagc cttcgacaat ctgctgcacg ggtgcctgtt
      601 tggagacagg cgcgagatcc tgacgcgtcg tgcgcaacag gctggccagc atatcttttt
      661 cgaaccggtc gccctgcacc tgactgcgtt tcaggcgcag caacatcaga tggccgtcga
      721 tttcgtcagg acgatctttc aggaccgagc tgaggagctc ctgagccagt tgcgacttgt
      781 cttcgaactc gtacgttcgc gccagctgat agccgacctg ataatcgttc ggcatgaccg
      841 ctcgtacctg cgtcagcagc ttcgagccgc gtcccaggtc tccgatgcgc agatagacat
      901 cagcgagaag ccgcatccag ccatgttcgc gtggcaccag cgccagagcg cgctcgaggc
      961 gttcgatggc ggtcagcaga aaccgtttct gaatggcatc gcgtgcctgg tcgaccaact
     1021 tgcggccctc tgcacgagcc ttctcgatgc gaatcaactc cggagcctga taggcgcgca
     1081 catgggtcgg atcaagtctg gccgcggctt caaaggcttt ctcggcgtca ggaaaccggg
     1141 caagacgaat cagggtttca ccgagatcgc accaggcatc gggggaagcg tgccccgcgt
     1201 cgatgaaggc cttctgtgct ttagcggtca cctcgtgtgc ttcgagtttg ccggcgcacc
     1261 tgatcaaccc ttgcagcgcc caggccgtgc cgggtgcccc ttccagaaac gcctcatact
     1321 cgctcagcgc tttcggccac aactcctttg attcgagtgc ttccgcgaag gttctgatga
     1381 tttcaggatg atgcggactc agttcccggg cccgttgcaa ctcacgcaga gccacatcga
     1441 tccggcggcg actcagatag tagcgcccga gatccagaaa gatttcgcct gaccggacat
     1501 tggcctcgcg aatgcgctga aagacttctt cggctcggtc ttccattccg gcttccccgg
     1561 acaaacgtcc gagttcaaaa agaatttcaa cccgatctgg agccgatttt gaggctcgtt
     1621 cgaattccag catcgcctga ccgcgttcgt tcgcggccag aagcgctttg cccagacccc
     1681 accgcaggtc ggggtcgtcc ggaagtgccg tcagagcctc tttatacaag ccgatggcgc
     1741 cgtcggcaaa gttctgagcc atcatggcgc gcccctgcaa caggtatgcc tcgcgtgagt
     1801 tgccggcgag tttgcgatac tgcgaaatgt cctcgagtgc gtcggcgggg ttgccgacgg
     1861 ccagctgaat ctcggcgacc cgcaagagca attcgggccg ttgttctgat tgtcggcgcc
     1921 agctctgcag acgagcaagc gcttcccggc gtcgcccgga ttcatagagc aggcgggcga
     1981 cctgcaatcc ggcttcgata tccgtctctg aaagcttgag acagcggtcc cagagatcga
     2041 tcgcttcgtc gcggcgtccg agagcgtcga gaatgcgggc acccatgcga aggatcgccg
     2101 ggtcctccgg agcgatggcg cgggcggctc gaaacgttga gtaggcgcga tcgaattttc
     2161 cgacgtgaaa gagcgcttcc ccgagattca gatacgccgc gggagagccc ggattagcct
     2221 tgagttcggc ggtcagctgg tcgagccgcg atgcctctcg ggtgctggtc accggggggt
     2281 cggcggcagg tgggggagag acggaaatct tgggcgctgg tacgggggag ggcgacgact
     2341 tgggggtggc ggcagccgaa gctggctcga gccgcgcccc gcagacgccg cagaaaatcc
     2401 ctcgagcatc cttgctgcca cagcttcgac actgggccat gattacatct cctgaggtgc
     2461 ggcaatgccg agcagatcga ggccgcgggc caacacgcga cgacacagac cgcagagtcc
     2521 gagtcgtgaa ttccgttctg cgccttctga ggccgtgaga accgggcact gatcataaaa
     2581 cgtcgagaat gcaacagcca gttcgaacag gtaggtgcac agcacatgcg ggcgcagatc
     2641 gacggtgacg gcgctgacga cctcggggaa ctgcagcagc ttcatggcca gggttcgttc
     2701 cgccggctgc gcgagacgaa actcattctt gacggcggtc tcgtcgatct tgcccttccg
     2761 gaagatcgag cagatgcggg catgcgcata ttgcagatag ggggcggtgt tgccgttcag
     2821 ggcgagcatg cggtcccacg agaacacgta gtcgttgttg cggttgttgc tcagatcagc
     2881 atacttcacg gcgccgatgc cgacagcttc ggcgacggcc tgttgccgct cggggctcag
     2941 gtcgccgttc ttttcgcgca ccacgttcag cgaacgctcg accgcttctg ccagaagatc
     3001 ggacaatttg acgttctctc cactgcgggt tttcagcggc ttgttgtctt cgcccatgac
     3061 gctgccgaac gtgacgtgtt cgagcggaat gcgcttgctg ttcttgttcg tccagccgca
     3121 ggtgtcggcg accgcgaaaa actgagaaaa atgcagggcc tgccgcgcgt cggtaacgta
     3181 aatgacgcgg tcggcatgaa gatcggcgat gcgatagcga acggcggcca ggtcgaaggt
     3241 ttcgtaattg tacccgccgt cagacttctg cacgatgatc ggaagcggtg atccgtcttt
     3301 ggttttgaac tgctgcagga agacgcactg cgcgccctgc gactcctcaa gctttcctga
     3361 ctctcgaatg acttgtactg tctgagcgag tcgatcgcga taaaaacttt cgccgcgttc
     3421 gtctttctcg gtcagggtga cgttcagcag acgatagata cgatacactt cggacatcga
     3481 aattcgggtg atggtttccc agtccttcac cagttgcggg tccccggcct gcagggatac
     3541 cagaaccttg cggcactctt cggctttcgc cgggtccgag gcgcattcct gcgtcacctg
     3601 tcggtagatt tcctcgagac gactcaggga aagctcctgc gcaggatcaa ggccgcgttg
     3661 ttcgagatcg cgcagaatcg gcgccgtttt ccagatgacc atggcgatcg gaacccccca
     3721 gtcgcccagg tggttctggc gaatgacggt gtaaccgagc gcttcaagaa cacgggcgag
     3781 aacgtcaccg atgatcgtcg atcgcaggtg gccgacatgc atctgtttag caatatttac
     3841 acttgaatag tcgatcacca caatctgtcc attgccggtg gctggtactc ccaggcgctg
     3901 atcacacgcc atgctggtca gagctttttc gattgacgcg tcggtgcaaa acaggttgac
     3961 gaaaccgggc ccgacgactt cgactttgcc gaaccattcc tcgatgggct tcaacagctc
     4021 gaccagacgc tgggcgatct tctgcggcgg ttgcttggct ggtttggcca gacgcatggc
     4081 cgtgttcaac gagaagtcgc cgaacttcgc ttcgggcgga cgctgcagct cgatgaaggc
     4141 ttcgggattc tcgatgttca attcgcggaa aagtgggaac aaagcctttt gcagaacggg
     4201 agagagcgtg tctttcatgc cgtggtcctt ccgatgaagt gtgcgagacg atgggtgttc
     4261 atagtcctag cgctgtgcat tccgaataag caccagtcca aaggttccga tgatgatatt
     4321 gggcagccag accccgatga atggggtgag ata
//
LOCUS       DAORHD010000242         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21584_length_4353_cov_3.218008, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000242 DAORHD010000000
VERSION     DAORHD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21584_length_4353_cov_3.218008"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /gene="uppS"
                     /locus_tag="PKO06_06445"
     CDS             <1..457
                     /gene="uppS"
                     /locus_tag="PKO06_06445"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNV69321.1"
                     /translation="LQNNIRFTAIGRPNVLPEDVYHELLQAEAKTSRNSGMRFNIALN
                     YGGRAEIVDAVRKIVREGIPPEQIDESTIAKRLYHPEVPDPDLIIRTSGEVRTSNFLL
                     WQSAYSELYVTPIYWPDFDEVEFLLALQHFQARERRFGKTGEQVRQTTT"
     gene            454..1248
                     /locus_tag="PKO06_06450"
     CDS             454..1248
                     /locus_tag="PKO06_06450"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNV69322.1"
                     /translation="MISRLVVVLIGIPAVYGLLVWHDFSRFALFAVAALLGQQELNRL
                     FAHPGSRPPILEYTLGVSMLWAAETQGERGLLLATAFGFILPGIAIVLRGLDYSGYKS
                     YSLGVTGLLYLPFSLSFLMMVAHISDAGGRTIFAVLCLIWALDIGAYLVGCTLQGPRL
                     APHISPKKTVSGAVGGVLACAIVAWSLRQIGWLGLSDLRLIALILCIGIVGQISDLFE
                     SVLKREAGVKDSGALLASHGGILDRIDSLLFVSPLVYAIFSMSSLR"
     gene            1267..2535
                     /locus_tag="PKO06_06455"
     CDS             1267..2535
                     /locus_tag="PKO06_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNV69323.1"
                     /translation="MITLGTLFAIIKGFLAICFVFGSIVLVHELGHFLTAKWSGIWVI
                     EFALGFGKRMVRFRIGDTLYSLRPFPLGGFVRLAGMDTPEDTEEKKPDEPGTDAEKAV
                     ETPPEPVDPDDVTPELAPDDPRGYPAKPRSLKMLVLAAGSIMNLVWAVVIFFIMFTVF
                     GGPLSNIYVTDATPGEPAALAGIRSGDVVTAINGTKIREWTDGIKVIQANGGSPITLD
                     VVRDHPEPRPGFGGGLVQMNRAVTPGMYELYRRETLQIKVTPTGGPGSGRIGIKLGAN
                     TYDFQVLALDHAATKAFESTRDIIAQTLGGLLRMLSRQTEADVAGPVKIMQMITQQSQ
                     KGFVDLLQMTALLSINIGLLNLMPFPVLDGGRILFLLIEAFLAFINFLTGLKLALDPK
                     WEENIHFIGMLFLLVVLVMVTVGDIRGLFH"
     gene            2688..3821
                     /locus_tag="PKO06_06460"
     CDS             2688..3821
                     /locus_tag="PKO06_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV69324.1"
                     /translation="MAEQALETLDGGVQQIVIRGEISDGEEELYRLIGKVAHKFPTLV
                     LNCLEVASVSPLFAQRLEEYRTMSRLKIVVGQSPVKEALADKGYLLFPSIKSAQLAIT
                     GEDTLRTILAKLKEAPILSAEAYQVLGYMNSPDADFDGVVKRVEKDQNICSQIVKVAN
                     SAFFFRSGKVDTLTQAVSRIGLLNIQNILVRSLYNGLTSFFQSQQDVIIHSQKCATLA
                     NYIAQEGGRPPDERAKIWLGALMHDVGRQAMAFVFPEKYAQVTELMTKENRPSYVAEL
                     YVFGTEHQVIGRLLAKKWNFPEYLTNIIGDHHSLAAKQWNTMTLPVFCANSFMNELEG
                     IPFVSYYQRLEGYYFMLKKDLPWKDVPTAFKAVVEAQFNDLLS"
     gene            complement(3919..>4353)
                     /locus_tag="PKO06_06465"
     CDS             complement(3919..>4353)
                     /locus_tag="PKO06_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69325.1"
                     /translation="VLSETTENLLFEEAVLKEILDTPPTFDESWCWAIMDVLDPFTGP
                     ISLFIPKPLVARFAEGVLAVPAEEISQELLLDGLAETLNTLAGRLVARRLSADESYKL
                     SLPATGVGEPILFAGESRYFHFLISDQFMFLQVTTALLKPRA"
BASE COUNT          864 a         1195 c         1285 g         1009 t
ORIGIN      
        1 gctgcagaac aacatccgtt tcacggccat cggccggccg aacgtgctgc ccgaggacgt
       61 ctaccacgag ctgcttcagg ccgaggccaa gaccagccgc aactccggca tgcgcttcaa
      121 catcgcgctc aactacggtg gccgggccga aatcgttgac gcggtcagga agattgtccg
      181 cgaagggatt ccgcccgagc agatcgatga gtcgacgatc gccaaacgac tgtatcatcc
      241 cgaggtgccc gacccggatc tcatcatccg caccagcgga gaagtgcgaa ccagcaactt
      301 cctgttgtgg cagagcgctt actcggaact gtatgtcacc cccatctact ggccggactt
      361 cgatgaagtc gagtttctgc tggcgctgca gcactttcag gcacgcgagc gccggttcgg
      421 caagaccggc gaacaggtgc gacagaccac gacatgatct cacgtcttgt tgtcgttctg
      481 atcggcattc ccgctgtcta tggcttgctg gtctggcatg atttcagccg ttttgcgctc
      541 tttgccgttg cggctctgct tggccagcag gaactcaatc gcctgtttgc gcacccgggt
      601 tcacgcccgc cgatcctcga gtataccctg ggtgtctcca tgctctgggc cgcggaaacc
      661 cagggggaac gtggtctgct gctggccacc gcgtttggtt tcattctgcc gggaattgcc
      721 atcgtcctgc gtggactcga ctactccggc tacaagagct attcgctcgg agtcacgggg
      781 ttgctctatc tgcctttctc cttgagcttt ctcatgatgg tggcccacat ttcggacgcg
      841 ggaggtcgta cgatctttgc cgtgctgtgt ctgatctggg cgctcgacat cggcgcgtat
      901 ctggtcggat gcacgttgca gggcccgagg ttggcacccc atatcagtcc gaagaagacg
      961 gtctcggggg cggttggtgg cgtcctcgcc tgcgcgatcg ttgcctggag tctgcgtcag
     1021 ataggctggc tcggtctttc cgatctgcgc ctgatcgctc tgattctctg catcggcatt
     1081 gtcggtcaga tctcagatct gttcgaatcg gtgttgaagc gcgaagccgg ggtgaaggat
     1141 tccggggcgt tgctggcgag tcacggcgga atactcgatc gtatcgacag tcttctgttc
     1201 gtcagcccgt tggtgtatgc tattttttcc atgtcgtcgt tgcgttgagg agtcgctgaa
     1261 tttgccatga tcacactcgg tacgctgttt gccattatca aagggtttct cgccatctgc
     1321 ttcgtgtttg gcagcattgt tctggttcat gaactgggac attttctgac cgcgaaatgg
     1381 tcgggcatct gggttatcga gtttgcgctt ggctttggca agcgtatggt gcgatttcgc
     1441 attggtgata ccttgtactc tttgcgaccc tttccgctcg gcggtttcgt gcgactggcc
     1501 ggcatggata ccccggaaga tacggaagag aagaagcccg acgaacccgg aaccgacgcg
     1561 gaaaaagcgg tcgagacacc gcctgaaccg gtcgatccgg atgacgtgac cccggagctc
     1621 gctcctgacg atccgcgcgg atatcccgcg aaaccccgca gtttgaaaat gctggtgctg
     1681 gccgctggct ccatcatgaa tctggtctgg gccgtcgtca ttttcttcat catgttcacg
     1741 gtgttcggtg gtccgctgtc gaacatctat gtcaccgacg cgacgccggg cgagccggcc
     1801 gcccttgcgg gaatccgttc gggtgacgtg gtaaccgcca tcaacggtac caagattcgc
     1861 gaatggacgg acggcatcaa agtgattcag gccaatggcg ggtctccgat tacgctcgat
     1921 gtcgtgcgtg accaccccga accacgcccg ggtttcggcg gcggcctggt gcagatgaat
     1981 cgtgccgtga cgccgggaat gtacgagttg taccgccgcg aaacgctgca gatcaaggtc
     2041 acaccgacgg gcgggcctgg ttcaggccgc atcggtatca agctgggcgc gaacacctat
     2101 gattttcagg tactcgcgct cgatcacgcg gcgaccaagg cgttcgagag cacacgtgac
     2161 atcattgccc agactctggg cgggttattg cgcatgctgt cccggcagac cgaagcagat
     2221 gtggccggtc cggtcaagat catgcagatg atcacgcaac agtctcagaa agggttcgtt
     2281 gatctgctgc agatgaccgc cctgttgtcg atcaacattg ggctgctgaa cctcatgcca
     2341 ttcccggttc tcgatggggg acgcattctg tttctgctga tcgaggcctt tctggcgttc
     2401 atcaattttc ttaccggttt gaaactggct ctcgatccga agtgggaaga gaatattcac
     2461 ttcatcggta tgctgttcct gctggtggtg ctggtgatgg taacggtagg ggacattcgc
     2521 ggtctgtttc attaaagagg cggggccctg ctttgctctc gaaatccgcg ttcgcgaaaa
     2581 agtttgctgg gacttttgct gcccctcttc tctttttttc tgatcctcgg tataatccag
     2641 gcgctcgctt ttttctcaca tctcggtagc tcggaggcag aggtcgcgtg gcggaacaag
     2701 cgcttgaaac acttgacggc ggggtacagc agatcgttat caggggcgag atctctgacg
     2761 gcgaagaaga actgtatcgc ctgatcggca aggtggcgca caagtttccg acgctggtgc
     2821 tcaattgtct cgaggtggca tcggtcagcc ctctcttcgc gcagcggctc gaagagtatc
     2881 gcaccatgag ccgactgaag atcgtggttg gtcagtcgcc cgtgaaagag gctctcgccg
     2941 acaagggcta tctgttgttt ccttccatca agagtgcaca gttggcgatc acgggtgaag
     3001 acacgctgcg cacgattctc gccaaactga aagaggcccc cattctcagt gctgaagcat
     3061 atcaggtgct gggatacatg aattcccccg acgcagactt tgatggtgtc gtcaagcgcg
     3121 tcgagaaaga tcagaacatc tgcagccaga tcgtcaaagt ggcaaactcc gctttctttt
     3181 tccgcagcgg aaaagttgat acgctgacgc aggcggtttc acgtatcggg ttgctgaaca
     3241 tccagaatat tctggtacgc agcttgtata acgggctgac gtcgtttttt cagagccagc
     3301 aggatgtcat tattcacagt cagaagtgcg cgaccctcgc gaattatatt gcgcaggaag
     3361 gcggacgccc accggatgaa cgagccaaaa tctggttggg cgcgctgatg catgacgtcg
     3421 ggcgacaggc catggcgttc gtctttccgg aaaaatacgc gcaggtaaca gaactgatga
     3481 ccaaagagaa ccgtccctcc tacgtggccg aactgtacgt gtttggcacg gagcatcagg
     3541 tgattgggcg cctgctggcg aagaaatgga attttcccga gtatctgacc aacattatcg
     3601 gcgatcatca ttcgctggcg gcgaaacagt ggaacacgat gaccctgccg gtgttctgcg
     3661 ccaattcgtt catgaacgaa ctcgaaggga ttcccttcgt gtcgtattat cagcgactcg
     3721 aaggctacta cttcatgctc aagaaagacc tgccctggaa agacgtgccg acggccttca
     3781 aggccgtcgt cgaagcgcag ttcaacgatc tgctgtcgtg atcgtctttg gggcgcgcga
     3841 acatgccctc tcggaaggca tttccatgaa atcgaattgg tacgattggc gaacgcgaac
     3901 gcagcgcttg aaggcgtttc aggctcgtgg cttcagcagg gcggttgtca cctgcaggaa
     3961 catgaactga tcactgatca ggaagtgaaa ataccggctt tctccggcaa acagaatcgg
     4021 ttcgccgact cccgtcgcgg gcaagctgag tttgtacgat tcgtcagctg aaaggcggcg
     4081 ggcgacgagc cgtccggcga gggtattcag tgtttccgcc agaccgtcca aaagcagttc
     4141 ctgcgaaatt tcctcggcgg gcacggccag aaccccttcg gcgaacctcg ccaccagagg
     4201 ctttggaatg aacagggaaa tgggtccggt aaacgggtcg agaacgtcca tgatggccca
     4261 gcaccacgat tcatcgaagg tgggcggggt atccagaatc tctttgagga cggcttcttc
     4321 gaacagcagg ttttccgtgg tctcggaaag aac
//
LOCUS       DAORHD010000243         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21618_length_4347_cov_4.244641, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000243 DAORHD010000000
VERSION     DAORHD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21618_length_4347_cov_4.244641"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PKO06_06470"
     CDS             complement(<1..848)
                     /locus_tag="PKO06_06470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69326.1"
                     /translation="MADINLKRLLRDLANPDDDLRALSAMTLMKVEVLDQAQREELFT
                     ALMKATRDKNIAVRFFSRRAIDKLRQDKDMEEGGAPAQRSIDAGLSSEAYEDRLQAVM
                     RIARENKSDYKDRLVAMLQTEHHDFVKASLISCLKLFLNKSEANILSPFLKDPDSRVR
                     SNTIEALEALKADGAIPLLFPCLEDTDNRIRAVAAKALQSFGEGKVFAVLKKMLGSND
                     EWMKFSAIHALSHINAGASIQMLVDAAKPPTQPDTRLRAIVALANFHDLTSYGFLKHL
                     VSGNEEP"
     gene            complement(1100..1909)
                     /locus_tag="PKO06_06475"
     CDS             complement(1100..1909)
                     /locus_tag="PKO06_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV69327.1"
                     /translation="MKHGWLFITLVLLVSCCTGAFAQLSYEGSSSMGEQLIPDLAKAY
                     EAKAGVKFDRITANDSDLGFKAVMEGKALLGGLSRMLKEEELKQNFMNQVVGYDAIAI
                     YVNKDNPVRNLTVEQIRKIFAGDIKNWKDVGGPDAKIVTVSRKGEEGGSSRHFLDIVM
                     EGKPPAAPTLEGADRGECVKKVVENKDAITYASIYFRKLGAEMCAVNGVLPEAEKLRT
                     GEYVISRPFNLIYKEADDQVCQKFVDFVFTEEGQKIVASYVMPVIDFSETK"
     gene            complement(1956..>4347)
                     /locus_tag="PKO06_06480"
     CDS             complement(1956..>4347)
                     /locus_tag="PKO06_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69328.1"
                     /translation="TLLNIFDEGLRSHDARLKANAIEAVGELELPMREIVSRIEPYLE
                     DPNNRIRANSVLALARGEPEKSRKVLVEMVESNDVQLRRSAAFILGQIPAQGNEPLAQ
                     KLIGDHSDDVRRRMVLSLKNFPADFAKSQIAIVLGDKNKWIRKHAVEIAAIYPEFPRD
                     LILRLLKTEANYPNIVACMDFFGRHPDPEAVRIIRTKVKDKREPVVSGAIRAIGAMQA
                     IPGLQAIAPQINYRDPQILRTFAMTLFNLGSLEIMDSILEKAGQIKKPPANDLYLPAL
                     DSCLESLNLGDKMPKNLMAELTRQASPAPAPVVPEPVVVQPVTPAAPIAPVAAVPPTP
                     PAMATPTPVTSPAPTPVAPPPSPPPAGEPAGTSSEAAEAASGTGEDISEELDLTELVG
                     LTKPETPVQPAAPAAAAPPPKPPRAPKPRQPEEFTAGVKFFNLGKYKKARASFIAALE
                     EKPDLAKAHYYLGMIAVEEKDWEAVRDSLSVYLETEPGNTKVCLALGRAYKMLRDWPG
                     VVRTLDQIIDAELAPKAQLRVYRDLGTGNIFLKKYAEARTLLEKAFHEDPSDPEVNFH
                     LAMSCFHLHDLERADTLLNDLLKHLSPDNRLYSMAESLQEKVRATMVTQGSSASDEEE
                     PDLPETPPPTIPSPVNGVYDLSDPGTSQTPDTVLEVKPTAPAEPEPAPDLTLLEPEPV
                     VPTPDKPTDSSEPQFLDLTDLTLDPPANQPGKPVTESVDLFDLSEVVTPQSETGEPVL
                     PDSTDLNFEPVEPKKPAPAKKPTQPARPKKPGPGDDSEGGGEPQGGKGPDFSLPSI"
BASE COUNT          808 a         1192 c         1362 g          985 t
ORIGIN      
        1 ggttcttcgt tgcctgaaac cagatgtttc aaaaaaccgt aggaagtcag atcgtgaaaa
       61 ttggcgagag cgacgatggc gcgcaggcgg gtgtcgggct gggtcggcgg cttcgcggca
      121 tcgaccagca tctgaatcga cgccccggcg ttgatatgtg acagggcgtg aatggcactg
      181 aatttcatcc attcgtcatt cgaaccgagc atcttcttca acacggcgaa caccttgcct
      241 tcgccgaacg actgcagggc tttggcggcc accgcgcgta tgcggttgtc ggtgtcttcg
      301 aggcagggga acagcagcgg aatcgcaccg tcggccttca gggcctcgag agcttcgatc
      361 gtattcgatc gcacccgact gtcaggatct ttcagaaacg ggctgaggat gttggcttcg
      421 ctcttgttca gaaagagttt cagacagctg atcaatgagg ctttgacgaa gtcgtgatgc
      481 tcggtctgca gcatggcgac cagtcggtct ttgtagtcgg acttgttctc gcgggcaatg
      541 cgcatgactg cctgcagtcg gtcttcatag gcttcagagc tgagaccggc atcgatgctg
      601 cgctgtgccg gcgcgccgcc ctcttccatg tctttgtcct ggcgcagttt gtcgatggcg
      661 cggcgcgaaa agaaccgcac ggcgatgttc ttgtcgcgtg tcgctttcat cagggcggta
      721 aacagctctt cgcgctgcgc ctgatcgagt acttcgacct tcatcagcgt catggcggaa
      781 agagctcgca gatcgtcgtc tgggttggcg agatctcgga gcaagcgctt cagattgatg
      841 tccgccatga ttcactcctc tgctgtctgg acaggttggg ttccggcaag aggcgcaaag
      901 aaccgcctca ttataggaac catggaaaac cctgtcaaga caacgttcac gccgacaggg
      961 tcgcagaatt ggctgggaac acacaatctt tcatcgattt gcatgagatt cggtcgattg
     1021 ctgcacgagc gagaagtcga acgcttgaaa aacgagagcc ggacgggaag gattcccgcc
     1081 cggctctcgt gtgaatcaac tacttggttt cggagaagtc gatgaccggc atgacgtagc
     1141 tggcaacgat tttctggcct tcttcggtga agacaaagtc gacaaacttc tgacacacct
     1201 gatcgtcggc ttccttgtaa atcagattga acgggcgtga gatcacatac tcaccggtac
     1261 gaagcttctc ggcttcgggc agcacgccgt tcacggcgca catttcagcg cccagtttgc
     1321 gaaagtagat cgacgcataa gtgatggcat ccttgttttc gacgaccttt ttgacgcact
     1381 cgccgcgatc cgccccttcg agtgtgggtg ctgccggagg cttcccttcc atgacgatgt
     1441 cgagaaagtg gcgcgaactg ccgccttctt cgcctttgcg tgacacggtg acgatcttgg
     1501 catcggggcc gccgacgtcc ttccagttct tgatgtcccc ggcgaagatc ttgcggatct
     1561 gttcgacggt cagattacga accggattgt ccttgttgac atagatggca atcgcgtcgt
     1621 agccaacgac ctggttcatg aaattctgct tcagctcttc ctctttgagc atgcgcgaca
     1681 aaccgcccag cagcgccttg ccttccatga cggctttgaa gccgagatcg gaatcattcg
     1741 ccgtgatccg gtcgaacttg actcccgctt tggcttcgta ggctttggcc agatcaggaa
     1801 tcaactgctc gcccatggag gaggaaccct catacgatag ctgggcaaag gctccggtgc
     1861 agcaacttac cagcagaacc agcgtgatga acaaccaacc atgtttcata ctcagcctcc
     1921 ctgaaggtgt atatgcagaa cgcatgtcgg cgcgatcaga ttgacggtaa tgagaagtcc
     1981 gggcccttgc cgccctgtgg ttcgccaccg ccttcgctgt cgtcgcccgg gcctggcttc
     2041 ttgggtcgcg ccggctgggt gggcttcttc gccggtgccg gtttcttggg ctcaaccggc
     2101 tcgaaattga gatcggtcga atcgggtaac acgggttctc ccgtttcgga ctgcggtgtc
     2161 actacctctg agagatcgaa caaatcgacg ctctcggtta ccggtttccc cggctgattt
     2221 gccggcggat caagcgtgag atccgtcagg tcgaggaact gcggttcact tgaatcagtc
     2281 ggtttgtccg gggtgggaac aactggttcc ggctccagga gagtgagatc gggggccggt
     2341 tccggctccg cgggggccgt cggtttgacc tccaaaacgg tatcaggcgt ttgactcgtg
     2401 ccaggatcgg acaagtcata cacgccgttc accgggctgg gaatcgtggg tggtggtgtc
     2461 tcgggaaggt cgggctcttc ctcgtcgctg gcggagcttc cctgcgtgac cattgtggcg
     2521 cgcacttttt cctgcaacga ctcggccatg gagtacaggc ggttgtcagg tgaaaggtgc
     2581 ttgagcaggt cattcaacag cgtgtccgcc cgttcgagat cgtgcaggtg aaagcaggac
     2641 atggcgagat gaaagttcac ctccggatcg cttggatcct catggaatgc tttctcgagc
     2701 agagtgcggg cttcggcata cttcttcaga aagatgttgc cggtgcccag gtcgcgatag
     2761 acacgcagtt gagctttcgg agccagttcg gcgtcgatga tctgatcgag cgtgcgcacg
     2821 acgcccggcc agtcgcgcag catcttgtag gcacgaccca gcgccaggca gacttttgtg
     2881 ttgcccggct cggtttcgag atagaccgac agactgtcgc gcacggcttc ccagtctttc
     2941 tcttcgaccg cgatcatccc aagatagtag tgggctttgg ccaggtcggg cttttcttcc
     3001 agggctgcga tgaagctggc acgagctttc ttgtacttcc ccaggttgaa aaacttcact
     3061 ccggccgtga actcttccgg ttgtcgcggc ttcggcgcgc gcggcggttt cggcggtggc
     3121 gcggccgcgg cgggtgctgc cggttggaca ggggtttccg gcttggtgag accgacgagc
     3181 tcggtcagat caagttcttc cgagatgtcc tcgcccgttc ccgaggcagc ctcggcggcc
     3241 tcggacgaag ttccagccgg ttcaccggcc ggtggcggag agggcggtgg cgcgacaggt
     3301 gtcggtgcag gagaggtgac cggtgtggga gtcgccatgg caggtggcgt cggtggcacg
     3361 gcggcaaccg gggcaatcgg tgcggctggt gtcacgggct gcacgaccac gggttccgga
     3421 acgaccggag ctggggccgg actggcctgg cgcgtgagct cagccatcag attcttcggc
     3481 atcttgtcac cgagattcaa cgattcaaga cagctgtcaa gcgctggtaa gtacaggtcg
     3541 ttggcgggtg gtttcttgat ctggccagct ttttcaagaa tggaatccat gatctcgaga
     3601 ctgccgagat tgaacagcgt catggcgaag gtgcgcagaa tctgcggatc gcgataattg
     3661 atctgcgggg cgatggcctg caggccggga atggcttgca tggcgccgat ggcacgaatg
     3721 gcgccggaga ccacgggctc gcgcttgtct ttcactttgg tgcggatgat ccgcacggct
     3781 tcggggtcag gatgtcgacc gaagaagtcc atgcaggcga cgatgttcgg gtagttcgcc
     3841 tcggtcttca acaaacgcag aatcagatca cgcgggaatt ccgggtagat ggcggcgatt
     3901 tcgacggcat gtttgcggat ccatttgttc ttgtcgccaa gcacgatggc gatctgcgac
     3961 ttggcgaaat cggccggaaa gttcttcagc gacagcacca tgcgccggcg cacgtcgtcc
     4021 gagtgatcgc cgatcaactt ctgcgccagt ggctcgttcc cctgggcggg aatctggccg
     4081 aggatgaacg cggcgctgcg gcgcagctgg acatcgttcg attcgaccat ctcgaccagt
     4141 actttccggc tcttttcggg ttctccccgt gccagtgcga gaaccgagtt cgcgcgaatg
     4201 cggttattgg ggtcttcaag atatggttcg atgcgggaga cgatctcgcg catgggcagc
     4261 tcgagttcgc cgaccgcttc gatggcgttg gctttgagac gcgcgtcatg gctgcgcagg
     4321 ccttcgtcga aaatgtttaa aagcgtg
//
LOCUS       DAORHD010000244         4345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21632_length_4345_cov_4.063170, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000244 DAORHD010000000
VERSION     DAORHD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21632_length_4345_cov_4.063170"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PKO06_06485"
     CDS             <1..1029
                     /locus_tag="PKO06_06485"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69329.1"
                     /translation="EALGEFLRREEDASLLATGVKIIGSFEQAEYIPVLRAFLTYEDE
                     RVRANAVESLASIKTAEVVDLLKALVSDRNNRVRANAIKGLWERGIRFGLNTLPEELR
                     SPNPRKRASVAYILGEIKDERSVELLVGLLGDVSPTVRNRAVISLGRIGASRVISQLL
                     TAYSREDDPPIRDRIIAVCMELNADLTLSRLIERYSNSDDHRFRANLIRSLALTGNGK
                     GLELFTRALRDEDSRVRANAIEGIGGLKNPALMEMLLPYLNDVNNRVRSNAATALWRL
                     GGMGAVVTLRQMLRSSHKLMRSSAAWALGEIGALQFSDVLQDLANDADPDVRKCALKA
                     LAKVSKIA"
     gene            1061..3676
                     /locus_tag="PKO06_06490"
     CDS             1061..3676
                     /locus_tag="PKO06_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69330.1"
                     /translation="MIRLHRLTLLGLFLTVMCAGSALTAWAQDAKELNRRGQLARQSG
                     NYKESMELLKQAMKLEPGWAEPYYNVYQLLKLVNKPEDSRRALKKAYELEPTNRTYQE
                     EYGKLLKEDLKAAEAKKDQAAIDALRKQITSVNPSELGLAADMVADLYKKKDLAGVKR
                     LAKELLEKNKAQLAQYDDPSLGRIFLLLARAEFDTNELKPAKEHAEKAANYPIEDPDE
                     AKALVGRVKQALQEEVAGHLRLGKSFSQKGETVAALAEFNKALAVDPQNDEAQSLIAE
                     LQAKADAKDLLAEVKKKSGQQKWLEVRDLLEVVVQNDPKNAEARTLLKKAVSIEEEAL
                     KKVGLTDKFPRDLIGLTDMVDSFLSKGKRFLEAGNKKDARQFFDRALVVITLNPDLEK
                     LREAVDVEVKKITSAEDRRSMYEKATRELYKTGDWEECLKLLEQLPQDYEPDMLSFMA
                     YCHWKLGNLDKAVDMAHIQLNRQSENNRARFVLGNIFHERGDNTAAYKYLNEIKQSDP
                     DYPGIDDVLMKVGAIKWGPLVIPVVAIVLLLWIAYIIYNNLDEYKKNSMIKKGRSFLN
                     RGYPRECIEELLKAKKLPNLDQYDGAVISRLLAQAYLKTGQYEKSVGECKHLLSINMN
                     DQEAHTWLGFCFLGLRRLNPESLPELLNLYKTEKNNQALIGLLGTHYASQKVLMPEGV
                     EILEKWLDNEPNNPDVLKTLGKFYLAKGRSDERSMKVFEQMMTSQKVDVDFVLGVAKM
                     HLRLKRYDACLKSCETVLSMDVNNDLVHQVLREAYREMGRIEELLEIYRGFLAENPYN
                     VAFQKGLTEAKKAFEKQGGRPAATESAPAEPIGAPEAAVEATGEAGEEILCPHCQKPN
                     NKADYYCQHCGKNIV"
     gene            3703..>4345
                     /locus_tag="PKO06_06495"
     CDS             3703..>4345
                     /locus_tag="PKO06_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69331.1"
                     /translation="MFRVSRYLVLCFCLVSGLLSAAPTSDYTTLKKFLAARMYAEAYY
                     ELLKCELNNKSEDPKLSKLKKDLLPNARKEATNRSKVRGDDPLVFTVLADLDYQENQL
                     ERGKANVTKALNGDSGTLTNYVFAKLLFAKGNMSQAADAMEKALAADPGLETVFEDFQ
                     FLYHCKNYGLAAAKRLSPDPGFLVRAKQVSSSEPPTPPDSPFENDPTRPPEVVT"
BASE COUNT         1069 a         1104 c         1283 g          889 t
ORIGIN      
        1 gaggcgctcg gtgaatttct gcggcgtgag gaagatgcta gtctgctggc gacaggcgta
       61 aagatcattg ggtcgttcga acaggcggaa tacattcccg tattacgcgc ttttctgacg
      121 tatgaagatg agcgggtgcg cgccaacgcg gtcgagtctc tggcttcgat caagacggcg
      181 gaagtggtcg atctgctcaa agccttagtg tcggatcgca acaaccgggt gcgggcgaat
      241 gccatcaagg gcctctggga acgcgggatc cgttttgggt tgaacaccct gcccgaggaa
      301 ctgcggtcgc cgaatccacg caaacgtgct tcggtggctt atatccttgg tgaaatcaag
      361 gacgaacgct ccgtcgaact gctggtggga ttactgggcg atgtctcgcc gaccgtgaga
      421 aatcgggcgg tcatcagtct cggtcgcatt ggggccagcc gcgtgatcag tcagctgttg
      481 acggcctatt cccgagagga cgatccgccg attcgcgatc ggatcattgc cgtatgtatg
      541 gaattgaatg ccgatctgac gctttccagg ctgatcgaac gctacagtaa ctcggacgat
      601 catcgatttc gcgccaatct gatacgcagt ctggccttga ccgggaacgg caagggcctg
      661 gaactgttca ctcgagcttt gcgtgatgaa gacagccggg tgcgcgccaa cgccatcgag
      721 ggcatcggtg gcctgaagaa tccggccctg atggaaatgc tgttgccgta tctgaacgac
      781 gtgaacaatc gcgtgcgcag taacgcggcg accgcgctct ggcgtctggg cggcatgggt
      841 gccgtcgtga ctctgcgaca aatgctgcga tcatcgcaca aactgatgcg ttcctcggcc
      901 gcctgggcgc ttggtgagat cggcgcactg cagttttccg acgtattgca ggatctcgcg
      961 aatgacgcgg atcccgacgt gcgcaaatgt gccctcaagg cgctggcaaa agtttcgaaa
     1021 atcgcctaaa ctaagccaaa ttccgcatgg gggattggct atgattcgcc ttcaccgcct
     1081 cacactgctg gggctttttc tgaccgtgat gtgtgctggt tctgcactga ccgcctgggc
     1141 gcaggatgcc aaggaactga accgccgggg acagctcgcc cggcagagcg gaaactacaa
     1201 ggagtccatg gaactgctga agcaggccat gaaactcgaa cccgggtggg cggaacccta
     1261 ctataacgta taccagctgt tgaagctggt caacaaaccg gaagactctc gccgggccct
     1321 gaaaaaggcg tatgaactcg agccgacgaa tcgtacctat caggaagaat atggcaagct
     1381 tctgaaagag gatctgaaag ccgctgaagc caagaaggat caagcggcga tcgatgcatt
     1441 gcgcaagcag attacctctg tcaacccctc tgaacttggt ctggccgcgg atatggtcgc
     1501 tgacttgtac aaaaagaaag atctggccgg cgtgaagcgc ctcgcgaaag agcttctgga
     1561 gaagaacaag gcacagcttg ctcaatacga cgatccgtcg ctgggccgta tctttctgct
     1621 gctggcacgc gccgagttcg atacgaacga actgaagccc gccaaggaac acgcggagaa
     1681 agccgccaac tatccgattg aagatcctga tgaggccaag gctctggtcg gtcgtgtgaa
     1741 gcaggcactg caggaagaag tagcggggca tctgcggctg ggcaagtctt tcagtcagaa
     1801 aggcgaaacc gtcgcggctc tcgccgagtt caacaaagct ctcgccgtcg atccgcagaa
     1861 cgatgaggcg cagagcctga tagcggaact gcaggccaaa gcggatgcca aagatctgct
     1921 ggcggaagtt aagaaaaaat cgggacagca gaagtggctg gaagtgcgtg atctgctcga
     1981 agtggtcgta cagaacgatc cgaaaaatgc cgaagcccgc accctgctga aaaaagccgt
     2041 gtcgattgaa gaagaagctc tgaagaaggt cggactcacg gacaagtttc cacgcgatct
     2101 gatcggtctg acggatatgg tggacagctt tctgtcaaag ggaaaacgat tcctggaagc
     2161 cggcaataag aaggatgccc gccaattttt tgatcgcgcc ctggtggtga tcacgttgaa
     2221 tcctgatctc gagaagctgc gcgaagcggt cgatgtcgag gtcaagaaga tcaccagtgc
     2281 tgaagatcgt cgcagcatgt atgaaaaggc gacccgcgag ctgtacaaaa ccggtgactg
     2341 ggaagagtgc ctcaagctgc tggaacagct gcctcaggac tacgaaccgg acatgctgag
     2401 tttcatggcc tactgccatt ggaagctggg aaacctggac aaggccgtcg atatggcaca
     2461 tatccagctg aatcgacagt ctgaaaacaa tcgcgcgcgg ttcgtgcttg gaaacatctt
     2521 tcatgaacgt ggtgacaaca cagcggcgta caagtacctg aatgagatca aacagtcaga
     2581 tcctgattat ccgggcatcg acgatgtgtt gatgaaggtt ggcgccatta agtggggccc
     2641 gctggttatt ccggttgtgg cgattgtctt gttgctctgg atcgcctata tcatttacaa
     2701 caacctggac gaatacaaaa agaactcgat gatcaagaag ggtcgctcct tcctgaaccg
     2761 cgggtatcct cgagagtgta tcgaggaact gctgaaggcc aaaaagctgc cgaatctcga
     2821 ccagtatgac ggggcggtta tctctcgcct gttggcgcag gcgtatctga agaccgggca
     2881 atatgagaag tcggtcggtg agtgcaagca cctgttgtcg atcaacatga atgatcagga
     2941 agcgcatacc tggctgggat tctgctttct tggtctccga cgactcaatc cagagagtct
     3001 tcctgagctg ttgaacttgt acaaaaccga gaaaaataat caggccctta ttggtctgct
     3061 cggaacgcac tacgcgtcac agaaagttct gatgccggaa ggtgtcgaga ttttggaaaa
     3121 gtggctcgat aacgaaccaa acaatccgga cgtactcaag acactgggaa aattctatct
     3181 ggcgaagggg cgtagtgacg aacgttccat gaaagtgttc gagcagatga tgacgtcgca
     3241 gaaggtcgat gtcgactttg tgctgggtgt ggctaaaatg cacttgcgtc tgaaacgata
     3301 tgacgcctgt ctgaaatcgt gtgagacggt tctgtcgatg gatgtcaaca acgacttggt
     3361 gcatcaggtg ctgcgcgaag cctatcggga gatggggcgc atcgaggaac tgttggagat
     3421 ctatcgcggc ttcctggcgg aaaacccgta taacgtcgcg ttccagaaag ggttgacgga
     3481 agcaaagaag gccttcgaaa agcagggtgg ccgcccggcc gcgacagaat ccgctccggc
     3541 agagccgata ggcgctccgg aagcggctgt ggaggcaacg ggcgaagccg gagaggaaat
     3601 tctctgtccg cactgtcaaa aaccgaacaa taaagccgat tattactgtc agcactgcgg
     3661 aaaaaacatc gtctgaaaat cgtctgcgtg tataaggaag gcatgttccg ggtttcgcgc
     3721 tacctggtcc tctgtttctg tctggtcagt gggctgctct cagccgcacc gacgagcgat
     3781 tacacgaccc tgaaaaagtt tctcgcggct cggatgtatg cggaagccta ctacgaactg
     3841 ctcaagtgtg aactcaacaa caagagcgaa gatccgaaac tctcgaagct gaagaaagat
     3901 ctgctcccga acgcgcgcaa agaggccacc aatcgttcga aggtgcgcgg cgacgatcca
     3961 ctcgtattca cggttcttgc cgatctcgat tatcaggaaa accagctcga gcgtggcaaa
     4021 gccaatgtga ccaaagctct caacggcgac tcgggcacgc tgaccaacta tgtctttgcc
     4081 aagctgctgt ttgcaaaagg gaatatgtcg caggcggctg atgccatgga aaaggctctg
     4141 gcggctgatc ccggtctgga gacggtgttt gaggattttc agtttctcta tcactgcaag
     4201 aactatggat tagcggctgc gaaacgattg tcgcctgacc ctggatttct ggtccgggcg
     4261 aaacaggtct cctcatccga gccgccgaca ccgccagaca gtccgtttga aaacgacccc
     4321 acccgtccac cggaagtggt gaccc
//
LOCUS       DAORHD010000245         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21664_length_4340_cov_3.666278, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000245 DAORHD010000000
VERSION     DAORHD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21664_length_4340_cov_3.666278"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="PKO06_06500"
     CDS             <1..1075
                     /locus_tag="PKO06_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69332.1"
                     /translation="PGHSSRASVSLPAHDVSATMMGENWCVPLSRWRWTLFLGFLVWF
                     LFIFIASFFSERFHVSRNLWWAIAFPLSALGPAFVQARRQMLDTALRLLPRRDQPMLD
                     LVQTYVQRHETGRDPAIVGLVSALAFGAFRVSASWEVRDILYSWPFIIHTVILLLGIG
                     CMAQTIWLILYYGRLINALTLRLSDQADRLFSWTQIAAMGNSYARASLGTALISLAFL
                     VMVLTNYQLFAADMKTHTVQILFMEVIFCAAVIMPFAYLILPQWRLRTLLITRKELLL
                     QRQLPLLIEAEHAMLIEDSRDVTRTERYLQRRREWREIEALPHWPFNIDAVAPMISVF
                     AIPVILFLFKEVLVDLIIDWVKG"
     gene            1077..2363
                     /locus_tag="PKO06_06505"
     CDS             1077..2363
                     /locus_tag="PKO06_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69333.1"
                     /translation="MSWVKKVLMIGTASILALMLSSPLFTVRAGFLDLFSSNQTTTPL
                     DKLSWQLHIEPENRLFPSYILAASTRAPLEATASDTPEIASGSLIIGEAVGPISITFT
                     VPAASLPVRLLVTGKAVMEDSVLETILPSPHQQIRLKPKILWKYPSLLEVRQAVPTDI
                     SAELWIGKQYLGRQTRTVKLHTINDCPFTFEENGEFTDTWWMYAAYVNENHPLVDSLL
                     KEAFAQKIVAQFDAYQSGDPKEVYRQIYAIWHLLYTKGIHYSSLTGSSDNAEKVGSQF
                     VRFLDESMSVNQANCVDGSVLFASILRKIGIDPFLVMTPDHMWIGFYLDEDRASKRFI
                     ETTQLGGLNEDEEAVDTELYHFFGEEYAERPSWQNFISAVRAGRDLYTENRNEIEAGE
                     SNHGVVDIEEARAMGVLPIAFTRQPPVSPSDPPSGR"
     gene            complement(2377..3138)
                     /locus_tag="PKO06_06510"
     CDS             complement(2377..3138)
                     /locus_tag="PKO06_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNV69334.1"
                     /translation="MKREFPILEFDEAREAVLNPRAKKGKTLPDRAVLCFFREVVERW
                     ARERQARVAHRITWEHGTHRIFEIEWNGSRIAFLQPGVGAPMAAGIMEELIPYGVRTF
                     VACGGAGTLRSELCVGHIILPSAAIRDEGTSYHYLPPSREIAAQPDVQTSLRRLLEER
                     GVPYVEGKAWTTDAPYRETKAKVELRKAEGCVAVEMEMAAMLAVARFRDVRFGQMLYA
                     GDDVGGETWDARGWSTRTDVREALFELALTACSRL"
     gene            3214..4110
                     /locus_tag="PKO06_06515"
     CDS             3214..4110
                     /locus_tag="PKO06_06515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69335.1"
                     /translation="MNHRLPVSYCGDPIRPPAEAESVILQLTTGCPHNHCDFCGAYQR
                     VPFRVRQPQEFRQHALWVREQEPLTHDLSRVFFADGDSLMLSPAALLEAIDIARSVFG
                     QARRFATYAGALGIRCKTGGELQQLKAAGLNTLYLGLESGSDFILTDMNKGASATEMV
                     EAVKHAQQAGLRVSVMVLLGLGGPERSREHALATAEALNRMQPRLLSVLTLMLIPGTL
                     LWQRAQQGSFRLLNQRETLVELRELLAALRLERTVFAANHASSFLPLTGALPRDQKHL
                     LAEIDAALAGQRRLVPDLLRGL"
BASE COUNT          835 a         1379 c         1164 g          962 t
ORIGIN      
        1 tcccggacat tcttctcgcg catccgtgtc cctccccgcc cacgacgttt ccgcgacgat
       61 gatgggtgag aactggtgcg taccactgtc tcgctggcga tggacgttgt ttctcggttt
      121 tctcgtctgg ttcctgttca tcttcatcgc ttccttcttt tcagaacgct ttcacgtcag
      181 tcgaaatctg tggtgggcga tcgcctttcc cctcagcgct ctcggacccg ccttcgtgca
      241 ggccagacgg cagatgctcg acactgcctt acgtctgctg ccgcgacgag accaaccgat
      301 gctcgatctg gtgcagacct atgtgcagag gcatgaaaca ggccgtgacc cggctatcgt
      361 cggtctggtc tcggctctgg cattcggtgc gttccgcgtc tctgcttcgt gggaggtgcg
      421 agacattctc tattcgtggc cgttcatcat tcataccgtg atactgcttc ttggcatcgg
      481 ctgcatggca caaacgatct ggctgatcct ctactacgga cgtctgatca acgcgctcac
      541 cctgcgcttg tctgatcagg cagaccggtt gttcagctgg acgcagatcg cagcaatggg
      601 aaactcctac gcccgcgcct cactggggac agccctgatc tcgctggctt ttctggtgat
      661 ggtcctcacc aactaccaac tgttcgcggc cgacatgaaa acgcacaccg tacagattct
      721 cttcatggaa gtcatcttct gtgcagcggt catcatgccc ttcgcgtatc tcatcctgcc
      781 gcaatggcgg ttacgcacgc tgctgatcac ccgcaaggaa ctgctgctgc aacgtcagct
      841 gcctttgctt atcgaagccg aacatgccat gctgatcgaa gattctcgcg atgtcacccg
      901 gacagaacgg tatctgcagc gccgacgcga atggcgcgag atcgaagccc tgccgcattg
      961 gccgttcaac atcgatgccg ttgcccccat gatctccgtc tttgccattc ccgtcatcct
     1021 ctttctcttc aaggaggtcc tggtcgatct catcatcgac tgggtgaaag gttgatatgt
     1081 cctgggtaaa aaaagttctg atgatcggta cagcgagcat actggccctc atgctgtcgt
     1141 cgccgttgtt caccgttcga gccggttttc tcgacctgtt cagcagcaac cagacaacaa
     1201 cgcctctcga caagctttcc tggcaactcc acatcgagcc ggagaatcgc ctgtttccct
     1261 cttacattct cgccgcctcg acccgcgcac ctctcgaagc cactgccagc gacacccctg
     1321 agatcgcttc aggcagcctg attatcgggg aagcggtggg gccgatctcg atcacgttca
     1381 ccgttcctgc ggcgtctctc cctgtgcggc tgctcgtcac cggaaaagcc gtcatggaag
     1441 acagcgtcct ggaaaccatt cttccatctc cgcatcagca gatacgtttg aagccgaaga
     1501 ttctctggaa gtatccctct ctcctcgagg ttcggcaggc cgttcccacc gacatttccg
     1561 ccgaactctg gatcgggaaa cagtatctcg gccgtcagac tcggacggtc aaacttcata
     1621 ccatcaacga ttgtcccttc acgtttgagg aaaatggcga attcaccgac acctggtgga
     1681 tgtatgccgc ctacgtcaac gagaaccacc cgctggtcga ctcgctgctc aaggaagcct
     1741 tcgcgcagaa gatcgtcgcg cagttcgatg cgtaccagtc cggagatccc aaggaggtct
     1801 atcgacagat ctacgcgatc tggcatctgc tctacaccaa gggcatccac tattcctcac
     1861 tcaccgggtc ttctgataac gcggaaaagg tcggcagtca gtttgtacga tttcttgacg
     1921 aatcgatgtc ggtcaatcag gccaactgcg tcgacggcag tgtgctgttc gcttccattc
     1981 tgcgcaagat cggcatcgac ccgtttctgg tcatgacccc ggaccacatg tggatcggct
     2041 tctacctgga cgaagaccgg gcctcgaaac gcttcatcga gaccacgcag ctcggtggtc
     2101 tgaatgaaga cgaagaggcg gtcgacactg agctgtatca ctttttcggt gaggagtatg
     2161 ccgagcgtcc ctcgtggcag aattttatct cggccgtgcg agccgggcgc gatctctata
     2221 ccgagaatcg caacgaaatc gaggcaggag agagcaatca cggagtcgtc gacatcgaag
     2281 aggcccgagc catgggtgtc ttgccgattg cgttcacgcg gcagccaccg gtttcaccgt
     2341 cggacccacc gtcgggccgc tagccctctc gcacgcctac agtcgactgc aagctgtaag
     2401 agccaactcg aacagcgctt cacgcacatc cgttcgtgtc gaccagccgc gagcgtccca
     2461 ggtttcgccg ccgacatcat cgccggcgta gagcatttgt ccgaagcgca cgtcacgaaa
     2521 gcgagccacg gcaagcatcg cggccatttc catttcgacc gccacacacc cttccgcctt
     2581 tcgcaactca actttggctt tcgtctcccg gtacggagca tcggtggtcc aggctttgcc
     2641 ttcaacgtag ggaaccccgc gttcctcgag cagtcgccgc aacgaagtct gcacgtccgg
     2701 ttgggcggcg atttcgcggg aaggaggcag atagtgatac gaagtgccct catcacgaat
     2761 tgcggctgag ggaagaatga tgtgcccgac gcagagttcc gagcgcaggg tccctgcccc
     2821 gccgcacgcg acgaaggtgc gcacgccata cggaatcagt tcttccatga tgccggccgc
     2881 cataggcgct ccgactcccg gctgcagaaa ggcaatgcgg ctgccgttcc actcgatttc
     2941 gaagatacga tgcgtgccgt gttcccaggt gatacgatgc gcgacccgcg cctgacgttc
     3001 gcgagcccag cgctccacca cttcgcgaaa gaaacagagc acggcgcgat cgggcagtgt
     3061 cttgcccttt ttcgcgcgtg ggttgagaac ggcttcgcgt gcctcgtcaa actccagaat
     3121 aggaaactcg cgtttcatgg acacctcgct caagcggcag atatcacatt ctaccgcacg
     3181 cggaaatctg ataccatggc cgcagtcaat cccatgaacc atcgtcttcc cgtctcctac
     3241 tgcggcgacc cgattcgccc gcccgccgaa gccgagagtg tgattctgca gctgaccacc
     3301 ggctgtccgc acaaccactg cgacttctgc ggtgcctatc agcgcgttcc ctttcgcgtc
     3361 agacagccgc aggagtttcg tcagcacgcc ctctgggtac gcgagcagga gcccctgaca
     3421 catgatctca gtcgagtgtt ctttgccgat ggcgattccc tgatgctttc ccccgcggcc
     3481 ctgctcgagg cgatcgacat cgcccgatcg gtgttcggac aggcccgccg ctttgccacc
     3541 tatgccggtg ccctggggat caggtgtaag acaggtggag aactgcagca gttgaaagcc
     3601 gctggtctga acaccctgta tctcggcctt gaaagcggtt cagacttcat tctcaccgac
     3661 atgaacaaag gcgcaagtgc caccgagatg gtcgaagccg taaaacacgc acagcaagcc
     3721 ggacttcgag tttccgtcat ggtcctgctc gggctgggcg ggcctgagcg ttctcgtgaa
     3781 catgcgctgg caaccgctga ggctctcaac cgcatgcagc cacgcctgct cagcgtgtta
     3841 acgctgatgc tgattcccgg caccctgctc tggcagagag cccagcaggg gagtttccgg
     3901 ctgctcaacc agcgcgaaac gctggttgag ttacgagaac tgcttgccgc gctgcgcctc
     3961 gagcgcaccg tcttcgccgc caaccacgcg tcatcatttc ttcctctgac tggcgctctt
     4021 ccgcgagatc agaagcacct gctcgcggag atcgatgccg cgctggccgg gcagcgtcga
     4081 ctggtccccg accttcttcg cggcctctag agcaagcgac atttttcttt cctttccgcc
     4141 gcttttcagc taaatatggg gaagtgtccg ttcggctgtc tcgtacagct gcatattctc
     4201 ttttgggagg cccatcatgg ctgacaacga tacctacacg gaagtaaccc acaaagggtg
     4261 gttcagtcgt ctcggtgact cgatcaaggg catcatcggt ggttttgtgc tcatgctcat
     4321 cgccgccggc ctgctgttct
//
LOCUS       DAORHD010000246         4334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21717_length_4334_cov_3.766534, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000246 DAORHD010000000
VERSION     DAORHD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21717_length_4334_cov_3.766534"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4334
                     /locus_tag="PKO06_06520"
     CDS             <1..>4334
                     /locus_tag="PKO06_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV69336.1"
                     /translation="HEVGLAHARSQVGNHLLIGADDGALYSLHPTTGALNWKFASRGG
                     VYVSSPSVDQTGTRIFFAPGDYDANLYCLNLSGTTHWTTPLLAPDMGGRIVVSSPTSS
                     GTHVFLQAAEVIPNLADPTAVHGFNIRPTVFCLNAADGSILWQVQPWPNYSFDNGFLS
                     PIAIVDDKLLVAGPDGSCRVLRASNGDVLGSVRTNQAVLATPLLSRNNLYLASTDGNL
                     YSLTGQDLTAPTGTILSPVSGSTVTSAVFDLRAQLNDPHSGINSSRINLLFGAESIPL
                     VYDAQTTIASASVECPFVYGTLVATLSFYNNAGLEQRLPWSVLVRDPSPLALLSAEAI
                     DEKTVRLTFNAPLASSAALVVGNFSLSAQSGTAPAIAAAAYESPTGLDPYRHLLLTLA
                     QPMPAGVTLTIQTQNQRSTDGRTLTTASVPFVGFTPNALIEALAIAPRQILLRFSSAL
                     ADSALTSPQKFAVSRMAGGQNVPITSLVFATKGPFTGATTLLITTGENLNDTSAYQVT
                     GTAVANANGSTISFVSRDFIGYVSPTLNSARVVAANGILLSFSDPLFSSTATSLPSYQ
                     LKRTSNGTVISLTQAITADPGTGDPHTGVLLTAASAPDPETYTLIASGLFALDGRVVA
                     TQTLDFSGDRTLRLVSANAVSPDAIVIRSDTALQFASITDTNLYSVQGFEPDSTPTVS
                     AVTFGDAWGFNPDRSIRLQFSAPLISSRYVIAITQVRDTTGKIFNNLSTPATGYVPLL
                     MSEASVAGNRQIMLTFNQSLATDSAQSLSQYTLTRLDTNQPVSLSAATLLPSAGNASA
                     AVLLESAADFTTTQYRVAIQNLRTAGDQVAANLQAEFSGAQAVRIASLYAPASRTLVL
                     SFDASLTLSAQDAARWELSSVSTGQIVPLVTGALDAAGRTLTLTTSADLGSQSYRLIG
                     RQLQTPSGTQISQLTATFAGYRPALLAWADSPDPRTVRLRFNAPPAPASIGTLAHYRL
                     VALAPAQVLTIVSAKPDPESQYGVLLLVRDNLPHTELEVTVTGLTSTEGAVTAFSSVR
                     IPARLRQNTVLTIEPIDFSSPSRYRLEVSSNAALSSENPVATIRYSGSTIALLLTRTE
                     NSAYQYLGEFDLPPNFAGLVSAEIEVKPGLRETVSLEIQPKYQLVRDGIVIPSLQAGL
                     FPDGYRVFFLPLSERLIREILPRLGLSSLAARIRGKLRAVGLVPSVLELDRLLAENHV
                     SGPWVLFTNDENDPTNLYSPLQLTLNLASFTTPQQIGLFQLHPSGEWDLLATPSAGNQ
                     LHALIHTTGVYALIADRDAPVVARTEPDNSGDTVPVLGNDQRLFVRLTEPKNTSGLAL
                     DSVRFIIDNESYLPTSIATDVSTTATQTAWNLEYRHPVPLAVRTFSWYLRAADRMGNQ
                     VADVMRQFAVADGLTLGEASLYPNPVRAGQSMHLRLRSTLRPDHLTITIRDTAGDRVA
                     LLVDEDAP"
BASE COUNT          799 a         1502 c         1128 g          905 t
ORIGIN      
        1 acatgaggta ggtcttgccc acgcccgtag ccaggtcggc aaccatctcc tgatcggcgc
       61 tgacgatggc gcgctctaca gcctgcatcc gaccacggga gccctcaact ggaagtttgc
      121 ctcgcgcggg ggcgtctacg tcagttcgcc ttccgtcgac cagacgggca cccgcatctt
      181 cttcgcgccc ggtgattacg acgccaatct gtactgtctg aacttgagcg gcacaaccca
      241 ctggaccaca ccactgcttg ccccagacat gggcggtcgc atcgtcgtct cgagtcctac
      301 ctcttcaggc acacatgtct ttctgcaagc cgctgaagtg ataccaaatc tggcagatcc
      361 aacggctgtc catggtttca acatccggcc cacggtgttc tgcctgaacg cagcagatgg
      421 cagcattctc tggcaggtac aaccgtggcc gaactatagt ttcgacaacg gctttctgtc
      481 gcccatcgcg atcgtcgacg acaaactgct ggtcgcgggc cccgacggaa gctgccgtgt
      541 cctgcgcgcc agtaacggtg atgtcctcgg ttcggttcgg acaaatcagg cggttctcgc
      601 cacgcctctc ctgtcgcgca acaacctgta tcttgcctcg accgacggca acctgtactc
      661 gttaaccggc caggatctca cggctccgac ggggacgatt ctctcgccag tttccgggtc
      721 tacggtgacc tccgcggttt tcgacctgcg cgctcaactg aacgatccac acagcggcat
      781 caacagctct cgcatcaatc tgctgttcgg tgccgagtcc attcctctcg tgtatgatgc
      841 gcagaccacg atcgcttcag cctctgtcga gtgcccgttc gtctatggca cgctcgtcgc
      901 aacgttgagc ttttacaaca acgccggtct ggaacaacgt ctgccatggt ccgtgctggt
      961 ccgggatccc agtccactgg cgctgctcag tgctgaagcc atcgatgaga aaaccgtgcg
     1021 actcaccttc aatgctccgc tggcttcgag cgccgcactg gtcgtgggca acttttcgct
     1081 ctccgcgcaa agtggaaccg cacccgccat cgccgcggcc gcgtatgaaa gccccacggg
     1141 tctcgaccca tatcgtcacc tgctgctgac gctcgctcag ccaatgcctg ccggggtcac
     1201 tctcaccatt cagacacaaa atcaacgttc gaccgacggg cgcaccctga ccacggcaag
     1261 cgtgccgttc gtcgggttta ccccgaacgc actcatcgag gccctggcga ttgctccacg
     1321 ccagatcctg ctccgattct cgagcgctct ggcggattcc gctctcacgt cgccacagaa
     1381 attcgccgtc tcacgcatgg caggcgggca gaacgttccc atcaccagcc tggtcttcgc
     1441 aaccaaaggg cccttcaccg gcgccaccac ccttctcatc accaccggcg aaaacctgaa
     1501 tgataccagc gcttatcagg tgaccggaac cgcggtcgcc aacgcgaacg gctcaaccat
     1561 cagcttcgtc tcccgcgact ttatcgggta tgtctcgccg acgctgaatt ccgctcgagt
     1621 cgtcgccgcc aacggcatcc tgctgtcgtt ttccgacccg ctgttttcct caacggcaac
     1681 cagcctgcct tcgtatcaac tcaagcgtac aagtaatggt accgtgatct cgcttaccca
     1741 agccatcaca gccgacccag gaaccggaga cccgcacacg ggagttctgc tgaccgcggc
     1801 ctcggcgccc gatcccgaga cctatacgct gattgcatca gggcttttcg cgcttgatgg
     1861 ccgggttgtc gccacgcaga ccctggactt ttccggtgac cggactctgc gtctggtctc
     1921 agcgaatgcg gtgagcccgg atgcgatcgt gattcgttcc gacacagcgc ttcaattcgc
     1981 ctcaatcact gacacgaacc tgtactcggt tcaggggttc gaacccgact cgacgcctac
     2041 cgtgagcgcg gtcacgttcg gtgatgcctg ggggttcaat cccgatcgct cgatccgtct
     2101 gcagttctcg gcaccactga tttcgtcacg ctatgtgatt gccattaccc aggtacgtga
     2161 tacgaccggc aaaatcttca acaacctgtc gacaccggcc acgggctatg tccccctgct
     2221 catgtccgaa gcatccgtgg cggggaaccg tcagatcatg ctcacgttca atcaatcgct
     2281 ggcaacagac tccgctcagt cactctcgca gtacacgctg acccgactcg acaccaatca
     2341 gccggtatca ctttccgcgg caacgttgct gccttccgcg ggaaatgcgt cagccgccgt
     2401 gttgcttgaa tcagccgctg acttcaccac gacccagtat cgtgtcgcca tccagaatct
     2461 acgcacggcc ggagaccagg tcgccgcgaa cctgcaggcc gagttttccg gagcccaagc
     2521 agtacgcatc gcctcgctct acgcccccgc ttcgcgcacg ctcgtcctct cgttcgatgc
     2581 ctccctgacc ctgtccgcac aggatgccgc tcgctgggaa ctgagcagcg tttccaccgg
     2641 tcagatcgtg cctctggtta ccggagctct ggatgccgcc ggacgaaccc tgaccctgac
     2701 caccagcgct gatcttgggt cacagagtta tcgattgatc ggccgccagc ttcagacccc
     2761 ctccggcact cagatttcac agcttaccgc cacgtttgcc gggtatcgcc ccgcgctgct
     2821 tgcctgggcc gattcccccg atccgcgcac cgtgcgtctg cgcttcaatg ctcctccggc
     2881 cccggcgtcg atcggcaccc tcgcccacta ccgtctggtg gcgcttgccc cggcacaagt
     2941 gctgactatc gtctcggcca agccggaccc ggagtcccaa tacggcgtgc tgctgctcgt
     3001 tcgcgacaac ctgccacaca cagaactcga ggtgaccgtc accggcctga ccagtacgga
     3061 aggagcagtc accgcttttt cttccgttcg cattccggca cggttgcgcc agaacaccgt
     3121 tctgaccatc gagcccatcg acttttcgag tccgagtcga tatcgactcg aagtcagcag
     3181 caacgcggct ctgtcgtctg agaatcccgt ggctaccatt cgttattcgg gttcgaccat
     3241 cgctctgctg ctgacccgaa ccgagaacag cgcttatcaa tacctcggag agtttgatct
     3301 accccccaac ttcgcgggac tcgtctcagc agagatcgaa gtcaaacctg gactgcgcga
     3361 gaccgtctcc ctcgagatcc agcctaagta ccagttagta cgcgatggca ttgtcattcc
     3421 aagcctccag gcaggcttgt tccctgatgg atatcgggtg ttctttctgc ccttgagtga
     3481 gcgcctgatt cgggagattc taccgcggct cggtctttcg tcgctggcgg cgcgtattcg
     3541 cggcaaactc agggccgttg ggcttgtccc gtcggttctc gaactcgacc gattgctggc
     3601 cgagaatcat gtctctggtc cctgggtcct gttcaccaac gacgagaatg atccgaccaa
     3661 cttgtacagt ccgctccagc tgaccctgaa cctggccagt tttacgaccc cgcaacagat
     3721 cggcctgttt cagttgcacc catccggcga gtgggatctg ctcgcaactc catcggccgg
     3781 aaatcagctt catgccctga tccataccac cggtgtctat gccctgatcg ccgatcggga
     3841 tgcgccggtc gtcgcgcgca ccgagcccga caactcgggc gacacggttc ccgtgcttgg
     3901 gaacgatcaa cggctgttcg tgcgcctgac cgaaccaaaa aacacctcag gactcgcact
     3961 cgactcggtg cggttcatca tcgacaacga gtcgtatcta ccgacgagca ttgccaccga
     4021 cgtgtcgaca acggccacgc agaccgcatg gaacctggaa tatcggcacc ctgtgcccct
     4081 cgccgtccga acgttttcat ggtatctgcg agcagccgac cgcatgggga atcaggtcgc
     4141 agatgtgatg cgccaattcg ccgttgccga cggtctgact cttggcgagg ccagtctgta
     4201 tcccaacccg gtgcgcgccg gccagagcat gcatctgcgg cttcgctcga cccttcgccc
     4261 cgatcatctg accatcacga ttcgcgatac ggccggcgac cgcgtcgctc tgctggtcga
     4321 cgaagatgcc ccct
//
LOCUS       DAORHD010000247         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21770_length_4327_cov_4.116339, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000247 DAORHD010000000
VERSION     DAORHD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21770_length_4327_cov_4.116339"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..177)
                     /locus_tag="PKO06_06525"
     tRNA            complement(86..177)
                     /locus_tag="PKO06_06525"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Ser,seq:gct)
     gene            complement(191..273)
                     /locus_tag="PKO06_06530"
     tRNA            complement(191..273)
                     /locus_tag="PKO06_06530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Ser,seq:tga)
     gene            complement(360..725)
                     /locus_tag="PKO06_06535"
     CDS             complement(360..725)
                     /locus_tag="PKO06_06535"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNV69337.1"
                     /translation="MTEEAPVKKRKIRFSTEVMFIVSVIFCVSLSIIYLSRYSLIRGI
                     QQREEEMKGKVMALERENRRLKQELQNLSTPEGIERLARERLGLVRPEEIVVVPLASG
                     TTLSFGTDTCPLPTAPGGR"
     gene            complement(743..3415)
                     /gene="gyrA"
                     /locus_tag="PKO06_06540"
     CDS             complement(743..3415)
                     /gene="gyrA"
                     /locus_tag="PKO06_06540"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV69338.1"
                     /translation="MVNDNETTVSGQQPPDQPPVQPPVPPNGGDGFPMVNIEDDMRAS
                     YLDYAMSVIVGRALPDVRDGLKPVHRRVLYAMYDQGFTSRRAYVKSARTVGEVLGKYH
                     PHGDQAVYDTLVRMAQDFSMREMLIDGHGNFGSMDGDSAAAMRYTEARLAPLAEEMLR
                     DLEMETVDWAPNFDGSLQEPIVLPAKFPNLLVNGSAGIAVGMATNIPPHNLSEVISGL
                     LRLIDNPELTIEDLMEDVQGPDFPTGGMIMGREGIRSAFTTGRGSVIMRSCVKIEDYG
                     KGRERIVVTELPYQVNKARLVAQIAELIKEKKVDGIVDLRDESNREGVRVCIELRKGV
                     NTEVILNQLYRHTPMQTSFGIIMLVLVDNRPLVLNLKQIMEYYVDHCRDVIRRRTTYQ
                     LRKAEEKAHILEGLTIALDNLDAVITLIREAASPVEAREGLISNFMLSEKQAQAILDM
                     RLQRLTGLERDKIVQEYHETLAEIARLKDILASDQKVREIIKTELLEIREKYGNPRRT
                     QLVASEAEQLDTEDLMQEEDILITVTKNGYIKRNKPDLFRQLNRGSKGSLVMGVKEDD
                     VVEHMFLGTTHSYLMVFTSTGKVHWIKGYKIPEGGRQASGRAIVNLLNLEEAERITAL
                     LPVKTFNDRRVVMVTRRGIIKVTELPAFSRPKSKGIIAINLDEGDELVSVKTIQGGEQ
                     IVIATRKGYAIRFPEQEVRTMGRTARGVKAISFRDADDSVVGMEAFTPGTGAPGVETS
                     VAADGTEVTTLPPESTLEPIPESALETEPDDEAEGEKVPYIPFEGRLLTVTAGGFGKQ
                     TDINEYRVTHRGGKGVINIRIRDRNGEVLAIKRMFDGDELMIVTTSGKVVRLNSDTIR
                     ETGRAAMGVKLITLNDTDKLISVVRIPAKEEIVV"
     gene            complement(3426..>4327)
                     /locus_tag="PKO06_06545"
     CDS             complement(3426..>4327)
                     /locus_tag="PKO06_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNV69339.1"
                     /translation="KAKLGNPEVRGIVDAVVTDFMTDYFEQNPHVAKPVIDKILNAMR
                     ARLAAQKARELTRRKTALDGGSLPGKLADCSEKEPKLCELFIVEGDSAGGSAKQGRDR
                     KIQAVLPLRGKILNVEKTRLEKVLGSEAIQDLVSAVGTSIGKDDFDLQRLRYHKVVIM
                     TDADVDGAHIRTLLLTFFYRYMPEIVDKGHLFIAQPPLYKVKKGKDERYAYSDDEMRE
                     IAEKMGGLEAVSLQRYKGLGEMNPDQLWETTMDPKNRILRRVVALDALEADRVFSVLM
                     GDKVEPRRQFISQYAKSVKNLDI"
BASE COUNT          913 a         1262 c         1139 g         1013 t
ORIGIN      
        1 ctcgaacctc tggccaccag atccgcaatc tggtgctcta tccaaactga gctacaggcg
       61 caatatgaac acttcaaaac agcggcggag aaggggggat tcgaaccccc ggtagaggtt
      121 taagccccta cgacggttta gcaaaccgtt gccttaagcc aactcggcca cctctcccca
      181 tgattgggca cggaggaagt aggattcgaa cccacgaacc tttcggtttc cggttttcaa
      241 gaccggcgcc atcgaccgct cggccattcc tccaaatcac gtatacctta ccacaggtac
      301 aaacgaaaat caagctccaa atacgctctg atttgggtta gcacctctct ttttttcgcc
      361 taccgtcccc caggggccgt cggtaatggg caggtatccg taccaaacga cagagtcgta
      421 ccactggcca gcggcacaac aacaatctct tcgggtctga ccaggccaag acgctcacgc
      481 gccagacgct caataccttc cggggtcgac aggttctgca gttcctgttt cagacgtcgg
      541 ttttcacgct cgagcgccat cactttccct ttcatttcct cctcccgctg ctgaattccc
      601 cgaatcaggg aatagcgcga cagatagatg atcgaaagag atacacagaa gatcacggaa
      661 acgatgaaca tcacttccgt tgagaacctg atcttccgtt tttttaccgg agcttcttcg
      721 gtcatgctgg ttcagtcgcc ggtcagacga cgatctcctc cttggcggga atacgaacca
      781 cggagatcag cttatcggta tcattcagcg tgatgagctt cacacccatc gccgcacgcc
      841 ccgtttcgcg aatggtatcg gagttcaacc gcaccacctt gccgctggtc gtcacaatca
      901 tcagttcgtc accgtcaaac atgcgcttga tcgccagcac ttcgccgttc cggtcacgaa
      961 tccggatgtt gatgacaccc tttccgccgc gatgcgtcac ccgatactcg ttgatgtcgg
     1021 tctgtttgcc aaacccaccc gcggttaccg ttagcaagcg gccttcgaac gggatgtagg
     1081 gaaccttttc cccttcggct tcatcgtccg gttctgtttc aagagcactc tcgggaattg
     1141 gttcgagcgt cgattcaggc ggcaaggttg tgacctcggt gccgtcggcg gcgaccgatg
     1201 tttccacacc aggagcgccg gtgccgggcg tgaacgcttc catgccgaca accgaatcat
     1261 ccgcatcgcg gaaggagatg gccttcacac cgcgagcggt gcggcccatc gtgcgtactt
     1321 cctgttcggg aaagcggatc gcatagcctt tgcgggttgc aataacaatc tgttcgccac
     1381 cctgaatcgt cttcaccgac accagttcgt cgccctcatc gagattgatg gcgatgatgc
     1441 cctttgactt cggtcgtgag aaggccggaa gttccgtcac cttgataatg ccacgtcgcg
     1501 tcaccatcac cacgcgtcga tcattgaacg tcttgaccgg caatagtgcc gtgattcgct
     1561 ctgcttcttc gaggttcagc agattgacga tcgcacgccc agaggcctgt cgaccaccct
     1621 caggaatctt gtaacctttg atccagtgta cttttccggt gctggtgaag accatcagat
     1681 atgaatgcgt cgtgcccagg aacatatgct cgacgacgtc gtcttccttg acgcccatga
     1741 ccagactgcc tttactgccg cggttgagct ggcggaacag atcaggtttg ttgcgcttga
     1801 tgtatccgtt cttcgtcacc gtgatcagga tatcctcttc ctgcatcagg tcttcagtat
     1861 ccagttgttc ggcttcagaa gccacaagct gggtgcggcg aggattgccg tacttttcgc
     1921 gaatttcgag aagctcggtc ttgatgatct cgcgaacctt ctgatcagat gccaggatgt
     1981 ccttcaggcg agcgatttcg gccagcgttt catgatattc ctgaacgatc ttgtcacgtt
     2041 cgagacctgt cagacgctgc agacgcatat cgagaatcgc ctgcgcctgc ttctcactga
     2101 gcataaagtt cgaaatcagg ccttcacgcg cctcgacagg tgaagccgct tcgcggatca
     2161 gcgtgatgac tgcgtcgaga ttgtcgagcg cgatggtcaa tccctcaagg atgtgcgcct
     2221 tttcttcggc tttgcgcaac tggtaggtcg ttcgccgccg gatgacatca cggcagtgat
     2281 cgacgtaata ctccatgatc tgcttgagat tcagaaccag cgggcgattg tcgaccaaca
     2341 ccagcatgat gatgccgaac gaggtctgca tcggggtgtg ccgatacagc tgattcagaa
     2401 tgacttcggt gttgacgccc ttgcgcagtt cgatacagac gcgaacgcct tcacgatttg
     2461 attcatcgcg caggtcgacg atgccatcga ccttcttttc cttgatcaat tcagcaatct
     2521 gagccaccag acgcgctttg ttgacctgat agggcaactc ggtcacgaca atgcgctcgc
     2581 gacctttgcc ataatcttca atcttcacgc acgaacgcat gatgacactg ccgcgacccg
     2641 tcgtgaacgc ggatctgata ccctcgcgac ccatgatcat accaccggtt gggaagtcag
     2701 ggccctgtac atcttccatc aagtcttcga tggtcagctc gggattatcg atcaggcgca
     2761 gaagaccgct gatcacttca ctcagattgt gcgggggaat gttggtcgcc atacctactg
     2821 cgataccagc agaaccgttc accagcaggt tcgggaactt tgcaggtagg acaatcggct
     2881 cctgcagaga tccgtcgaag ttcggtgccc aatcgacggt ctccatctcg agatcgcgca
     2941 acatctcttc ggcaagcggt gccagacgcg cttcggtata acgcatggcc gcagccgagt
     3001 cgccatccat cgaaccaaag ttcccgtgac cgtcgatgag catttcgcgc atcgagaaat
     3061 cctgcgccat gcggaccagg gtgtcgtaga cggcctgatc accgtgcgga tggtatttac
     3121 cgagcacttc accgaccgtg cgtgcggatt tcacatacgc tcgtcggctg gtaaagccct
     3181 gatcatacat cgcgtagaga acccggcggt gaaccggttt cagaccatcg cgaacgtcag
     3241 gaagcgcgcg accgacgatg acgctcatcg catagtcgag atacgacgca cgcatgtcgt
     3301 cttcgatatt taccatcggg aagccatcgc caccgttcgg tggaacggga ggctggaccg
     3361 gaggttgatc gggaggttgc tgaccgctga cggttgtttc gttatcgttc accatatgag
     3421 ggtcctcaga tatccaggtt ctttacggat ttggcatact gcgaaatgaa ctggcggcgt
     3481 ggctcgacct tatcgcccat cagtacagaa aacacccgat cggcttcgag cgcatcgagc
     3541 gccacgacgc ggcggaggat gcgattcttc ggatccatgg tggtttccca cagctgatcg
     3601 gggttcattt cgccaagacc tttgtaacgc tgcagggaaa cggcttcgag gccgcccatt
     3661 ttctcggcga tttcacgcat ctcgtcgtcg ctataggcgt aacgctcatc cttgcctttt
     3721 ttcactttat acaaaggcgg ttgggcgatg aatagatgcc ctttgtcgac gatttcgggc
     3781 atatagcgat agaagaaggt caacaacagc gtacgaatgt gcgctccgtc aacgtcggcg
     3841 tcggtcatga tgacgacctt gtgataccgc agcctctgca aatcgaagtc atccttgccg
     3901 atgctggtgc cgactgctga gacgagatcc tggatggctt cgctgccgag aaccttctcg
     3961 aggcgggttt tctcaacgtt gagaatctta ccgcgcaacg gcaacactgc ctgaatcttg
     4021 cggtcacgcc cctgtttggc tgaaccaccg gccgaatcgc cttcgacgat gaacagttcg
     4081 cacagcttcg gctctttttc ggaacagtcg gcaagtttgc ccgggagcga accaccatcg
     4141 agtgcggtct ttcgccgagt cagttcacgc gctttctgag cggcaaggcg ggcacgcatg
     4201 gcattgagaa tcttgtcgat caccggtttt gccacatgag gattctgttc gaagtaatcg
     4261 gtcatgaaat cggtcacaac cgcatcgacg atgccgcgca cctcggggtt gccgagtttg
     4321 gctttgg
//
LOCUS       DAORHD010000248         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21772_length_4327_cov_3.699438, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000248 DAORHD010000000
VERSION     DAORHD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21772_length_4327_cov_3.699438"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PKO06_06550"
     CDS             complement(<1..1443)
                     /locus_tag="PKO06_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV69340.1"
                     /translation="MTTRTTSIDTKPVISLSLLRELSQAISHMTPDRAMRVILENAVA
                     ATESSGAVLLIPHEKQREMVQLFEHLPEPLPANFWEPLLSSGMYRLFTEGRTRLLEAG
                     QLRDYLASAADTLSQALAMPLQPAGKPIGMLFLFRGRGSTVGFSGEHLDFIDILAPFF
                     GTLIENFRLHNEMLHKNTRLSALYEISQRAESLIDLRDIYDALGKVVKSFIDYDVFML
                     HLLLSDGQTLENRGSTTGPFPTQVKIGEGPLGQAAKELKPHLTYTDQYKSVLILPIVV
                     SGKLIGVLTIASLKSYAYRYEDIIGLRIIATQIASIDVLFKDLLRLRGFTQHILQSMT
                     SGVLIFDNQGKVTLSNPALTALIGQVIPEGWSAAREDNSLPSSLRKILLEVSDSKTAV
                     ENMKVQLSELSPPATIEVNAFPFRDESGLMLGTAFFFKDITQIVRLEDQLKRADRLSA
                     LGALAAGIAHEIRNPLTGMKMIVQLLQADFP"
     gene            complement(1475..2494)
                     /locus_tag="PKO06_06555"
     CDS             complement(1475..2494)
                     /locus_tag="PKO06_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV69341.1"
                     /translation="MTHVTSPRRILISRTDRAGDLTLTVPVFQALKTLQPECHLTAHL
                     RTYTAPLLRGHPAVDQLIVDDPEGGLSLLALARTFRESQFDAAILVHPSMRAILAAFL
                     AGIPLRIGRASNLWQIFLNRRAHQHRSRNEKHEYQYNLDLLNALDIAPPDIAPRLFPD
                     PALLPAVRQALARAGLPNARPWFVHPGHGGSALNLPLDQYAKLARELVQREIPVCVTL
                     GPGESALSQAFGQAIPDRFGFVTDLPDLSYVVALLSLGRGWVGGSTGPMHLAAAVNLP
                     VAAFFPPLPAMTPKRWGPVTRPAQVFLPAIPQCPQHCSRCPSHPCMNTLDTLPVVEWI
                     SRHAL"
     gene            complement(2491..3492)
                     /gene="waaF"
                     /locus_tag="PKO06_06560"
     CDS             complement(2491..3492)
                     /gene="waaF"
                     /locus_tag="PKO06_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNV69342.1"
                     /translation="MKTLVIGLNWLGDIIMSFPALALDHERTPTTLSVVTRPHLAAAY
                     LLHPHPLDVIPLDTSQSLWAQIPTIRRLRREQYDQIIVFPRSFRTAFIARLCGGTTRI
                     GFAAEGRSWLLHQALPLPEDLVTRHESTLYQILVREAGLTCLSECPSARLPSAPQQEE
                     LFTRLGLKRQRYLVLAPGAAFGPAKRWPPQHFREIAQRWVREYQTPVVLTGSVGEAEL
                     TSVVRNNTSDLIIDLAGKTSIAELTVLLSQATAMIANDSGTMHLGALTRTPMVIPIGP
                     TTPERTGPLSDCATLVRARRDHEPCRQRTCPRQGLSCLEQLPPDEVWAALQARVRQP"
     gene            complement(3489..>4327)
                     /locus_tag="PKO06_06565"
     CDS             complement(3489..>4327)
                     /locus_tag="PKO06_06565"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV69343.1"
                     /translation="VHLVSGLRDLVPLAHRLRRDGVSARSTYDLQGKVLSHLLCLLMG
                     GSAGRYTKRSLSESWHAWRGRYPLPSANPDPVWRRYLATVQADTVEQPDARLIITDNY
                     LRECREFLTALDPALASSYVAFHPGASHPGKRFTPENLQALVSQLHGTLLFIGDQPDP
                     PAPQMRSAQIIDGRGRIPLRLLPGTLRLSQGLIGTDSGPMHLARATGTPLVACFFQTD
                     PCLGFAPIPGNLQYLISRPLPCKPCSLHGQRTVCPVGTWACRHIDWVAEAAAFKSLLY
                     KS"
BASE COUNT          953 a         1121 c         1436 g          817 t
ORIGIN      
        1 cgggaaatcc gcctgcagca gctggacaat catcttcatg ccggtcagag gattgcgaat
       61 ctcgtgcgcg ataccggccg caagcgcccc aagcgccgag agccgatcgg cacgtttcaa
      121 ttgatcctcg agtcgtacga tctgggtaat gtccttgaag aagaacgccg tgcccagcat
      181 tagcccgctc tcatcgcgaa atggaaaagc gttgacttcg atggttgccg ggggagacaa
      241 ctcactgagt tgtactttca tattttcgac ggcagtcttg gaatcgctga cctcgaggag
      301 gatcttccgg agcgacgagg ggagcgagtt atcttccctg gccgcggacc acccctccgg
      361 aatgacttgc ccgatcagcg cggtgagcgc tggattcgac agggtgacct tgccctgatt
      421 gtcgaagatc aggactcccg aggtcatgct ttgcagaata tgttgagtaa aaccgcgcag
      481 tcgcagcaga tctttgaaga gcacgtcgat cgaggcgatc tgtgtcgcga tgatgcgcag
      541 tccgatgatg tcttcgtagc ggtaggcata ggacttgaga ctggcgatcg tcaagacgcc
      601 aatcagtttg cctgacacga cgatggggag gatcagaaca gatttgtact ggtcggtata
      661 cgtcaggtgc ggcttcagtt ccttggctgc ctgcccaaga ggtccctctc caatcttgac
      721 ctgtgttggg aatggtccgg tggtcgatcc gcggttctca agtgtttgtc cgtcgctgag
      781 cagcagatgc agcatgaaga catcgtagtc gatgaagctc ttcacgactt tgccgagagc
      841 atcataaatg tcacgcagat cgatcagcga ctcggcacgt tgcgagatct cgtagagggc
      901 tgagagacgc gtgtttttgt gcagcatctc gttgtgcaga cgaaaattct cgatcagcgt
      961 gccgaagaac ggtgccagaa tatcgatgaa atcgaggtgt tctccggaga agccgacagt
     1021 cgagccgcgg cctcggaaca agaacagcat tccgatgggt tttcccgctg gttgcagcgg
     1081 catggcaaga gcctgcgaca gagtgtcggc agctgaagcc agatagtcgc gcagctgacc
     1141 ggcttcgaga aggcgggtgc gaccctcagt aaacaatcga tacatgccgc tggagagcag
     1201 tggttcccaa aagttggcgg ggaggggctc cggcaggtgt tcgaacagtt gcaccatctc
     1261 gcgttgtttc tcatgcggaa tcaggagtac ggctccgctt gattccgtcg cggcgacagc
     1321 gttttccaga atgacccgca tggcgcgatc gggggtcatg tgggaaatgg cctgcgaaag
     1381 ttcacgcagg agagacagac tgataaccgg cttcgtatcg atgctcgtcg tgcgggtggt
     1441 catgaaagtg ccctcgccat gcgctgctac cctatcacaa agcgtgacgg gaaatccact
     1501 cgacgacggg gagcgtatcg agggtgttca tgcacgggtg cgatggacac ctggaacagt
     1561 gctgaggaca ctgcggaatc gccggcagaa acacctgcgc cgggcgagtg accggacccc
     1621 agcgtttcgg agtcatggcc ggcagcggcg gaaagaatgc cgccacgggc aggtttacag
     1681 ccgccgcgag atgcatgggc ccagtcgaac cgccgaccca gccacgcccg agagacagca
     1741 gagcgaccac atacgagagg tctggcagat cggtgacgaa gccaaatcgg tcaggaatgg
     1801 cctggccgaa agcctgcgac agagccgatt ctcccggccc cagcgtgaca cagacgggaa
     1861 tctcgcgctg gaccagttcg cgagcgagtt tggcatattg atccagaggg aggttcaggg
     1921 ctgaaccgcc gtgaccggga tgcacgaacc agggccgtgc attcggcaga ccggcacgtg
     1981 ccaacgcttg tcgcactgcc ggaagcaatg cggggtcggg aaacagacga ggggcgatgt
     2041 cgggaggagc gatgtcgagc gcgttcaata gatcgagatt gtactgatat tcatgttttt
     2101 cgttacggga acgatgctga tgagcccgtc gattgagaaa aatctgccag agattcgatg
     2161 ctcttccgat gcggagtggg atgcccgcaa gaaacgcggc gagtatcgcg cgcatcgacg
     2221 gatggacaag aatggcggca tcgaattgag attcacggaa ggttctggcc agcgccagca
     2281 gggacagacc accctcagga tcgtcgacga tgagttggtc taccgctggg tgaccgcgca
     2341 gcaggggggc agtgtaggtg cgcaggtgtg ctgtcaggtg gcattctggc tggagcgttt
     2401 tcaacgcttg aaagacgggc acggtcaatg tgagatcgcc tgcccgatcg gtacgagaga
     2461 tgagaattcg tcgcggagaa gtaacgtggg tcatggctga cgtactcgcg cctgcaaggc
     2521 tgcccagact tcatcggggg gcaactgctc gaggcaggaa agcccctgac ggggacaggt
     2581 tcgctgccga cacggctcgt ggtctcgtct cgctcgaacg agagtcgcgc agtccgagag
     2641 cgggccagtc cgctccgggg tcgtcggtcc gatcggtatt accatgggag tgcgtgtcag
     2701 agcgccgaga tgcatggttc cggaatcgtt ggcaatcatg gctgtcgcct gactcaacag
     2761 aacggtgagt tccgcgatac tggttttgcc cgcaaggtcg atgatcagat cagaggtgtt
     2821 gttcctgacg acagacgtca attcagcttc tccgactgac cccgtcagga ccaccggtgt
     2881 ctgatactcg cgcacccagc gctgggcaat ctcgcggaaa tgctggggtg gccagcgctt
     2941 tgcggggcca aatgccgcgc caggagcgag gacaagatat ctctgtcgtt tcagtccgag
     3001 tcgtgtgaac agttcttcct gttgcggagc agatgggaga cgtgccgaag gacactctga
     3061 caggcaggtc agtccggcct cgcgcacgag tatctgatac agcgtgcttt cgtgccgcgt
     3121 gacgaggtct tcaggaaggg ggagcgcttg atgcagcagc cagctgcgtc cttcggcagc
     3181 gaatccgatg cgtgtggttc ccccgcagag tcgggcaatg aaagccgtgc gaaacgaacg
     3241 ggggaagaca atgatctgat catactgttc acgtcgtagc cggcgaatag tggggatctg
     3301 cgcccacaga ctctgcgagg tatcaagggg aatcacatcc agcggatggg ggtgcaacag
     3361 gtaggctgcg gcgagatgag ggcgggtgac cacggagaga gtggtcgggg tgcgctcgtg
     3421 atcaagcgcg agggccggga atgacatgat gatatcgcca agccaattca aaccaatgac
     3481 gagtgttttc atgacttgta caaaagagat ttaaacgcgg cagcctcggc gacccagtcg
     3541 atgtggcgac aggcccaggt gccgaccgga caaaccgtgc gctggccgtg gagactgcag
     3601 ggtttgcacg ggagtggccg ggagatgaga tactgaagat ttccggggat gggagcgaag
     3661 ccgaggcatg gatcggtttg aaagaagcag gcgaccaggg gcgtgccagt ggcacgcgcc
     3721 agatgcatcg gaccggaatc ggtgccgatc aggccttgcg acagacgaag agttccggga
     3781 agcaggcgca ggggaattcg tccacgaccg tcgatgatct gtgccgaacg catctgagga
     3841 gcagggggat cgggttggtc gccgatgaac aagagtgtgc catgcagctg ggatacgagt
     3901 gcctgaagat tttcgggcgt gaagcgcttg ccggggtgcg aagcgcccgg gtgaaaggcg
     3961 acataggacg aggccagagc cgggtcaagg gccgtgagaa actcccggca ctcgcggaga
     4021 taattatctg tgattatcag cctggcatcg ggctgttcaa cagtgtcagc ctggacagtc
     4081 gccaaatatc ttcgccagac cgggtctggg ttggcagatg gcagtggata tcgtccgcgc
     4141 caggcatgcc aggactcgga caaggaacgt ttggtgtaac ggcctgccga gccccccatc
     4201 aggagacaaa gcaggtgtga cagaaccttc ccctggagat cataggtcga acgtgccgag
     4261 acaccgtcac gtcgcagacg atgtgccagg ggaacgagat cgcgcagacc tgatacgagg
     4321 tgtacct
//
LOCUS       DAORHD010000249         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21775_length_4327_cov_2.903792, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000249 DAORHD010000000
VERSION     DAORHD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21775_length_4327_cov_2.903792"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..133)
                     /locus_tag="PKO06_06570"
     CDS             complement(<1..133)
                     /locus_tag="PKO06_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014523408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV69344.1"
                     /translation="MADSLDGALTIFKPAGVTSHDVVDRVRRAFGVRAGHAGTLDPMA
                     "
     gene            complement(126..1118)
                     /locus_tag="PKO06_06575"
     CDS             complement(126..1118)
                     /locus_tag="PKO06_06575"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNV69345.1"
                     /translation="MHTREREALFAPIRAVLASAHSVLSIGHPDGDGDSIGSQLALHH
                     FCRALGKRSVPLNFDPIAPSISWLAGCGDFAGGLDDGEHFDVIFLMETTDIGRMGDRA
                     RFFTRATTRIHLDHHVGVKGLGDLNVLDDEASSTCEILFDLLEGMDHQLTIPSLEALY
                     VGIMTDTGNFRFPNTTLRSHEIAGQMLTRGVEATRLYKLVYEANQVTRILMHGTAMSR
                     VTTRCRGKLQYTWLTREDFTRHGATNVDADGAINPLCTIVGTDVALLFRELESGQIKV
                     SLRSTGRFDVCVVSRTFGGGGHRLAAGVQIAGPLERAIDNLLQVIEPELEKLDG"
     gene            complement(1124..1498)
                     /gene="rbfA"
                     /locus_tag="PKO06_06580"
     CDS             complement(1124..1498)
                     /gene="rbfA"
                     /locus_tag="PKO06_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNV69346.1"
                     /translation="MKHHRLERVASVLQHEIARIISQRIKDPRVQGMHIVSVDVSPDL
                     HLARINYSMLDQEGDPEAMQKGLDSAKSAIRAELKKVLQLRILPELAFFFDPSIRQGD
                     RLLELLRQIKPASPPESGEPGK"
     gene            complement(1495..1782)
                     /locus_tag="PKO06_06585"
     CDS             complement(1495..1782)
                     /locus_tag="PKO06_06585"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HNV69347.1"
                     /translation="MAMLVAIGTYDLHFPELHSLKEKRQVLRCLIDRVRAKFNASISE
                     VEDHDLWQKGTLGVALVSNDQTLLQQMGQKIDDIITDHDQVQVLNRHWEYV"
     gene            complement(1800..4295)
                     /gene="infB"
                     /locus_tag="PKO06_06590"
     CDS             complement(1800..4295)
                     /gene="infB"
                     /locus_tag="PKO06_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNV69348.1"
                     /translation="MRLHEAVKQFKTERKDLVALLAKLGFQVKDNALAAVSEDMVSAL
                     EKHFSGAGKTAPAAAPAKPTAAKAEPTATKPPAKPVVREPKQEVRMITKVEPKPTQTP
                     QPANRPATPTQPAQQTTRPAQPQQAPARPITPVGQTVPATAQQPQRPATPPQTPRPMF
                     PTPIQIVQPAKDKKTGPKPVRPIGEEGDGGITIIKTAPVKKEGPPKKNKKMKNRGQLP
                     QKATEESRGEALVIEGSAAVAQVAPGTPFAPQPVVKRITLPQEVTVNELAKYLQVEAV
                     EIIKKLMTMGVFASLNQRLERDHVETLAAEYGKEITFSEDVVDLEETPDVDTDLKLRP
                     PVVTIMGHVDHGKTSLLDRIRHAKVAEGEIGGITQHIGAYQVKSATGSITFLDTPGHE
                     AFTAMRAQGAQITDIAILVVACDDGVQPQTVEAIHHAQAANVPIIVALNKVDKPDANA
                     EKAKQQLMPYNLVSEDWGGKTIMVPVSAKTGVGVDQLLEMILLQAEIMELKANPNREG
                     QGTIIEAKLDKGMGPMGTLIIRKGTIRVGDSIICGTCYGRVKALIDDTGSRIKEAGPS
                     TPVVVLGLNEVPRVGDRLVVVESTKFARYVGVLRLKQEREERLERDNRLKLTNLFARV
                     AEGNVKELNLIIKADVQGSSGALKDALERLSNPDIKVNVIHAGVGAITESDVMLAAAS
                     NAIIIGFHVRPTHGVDEVSQRENVEIKVFRIIYDAIDAVKLALKGMYEPEYEEEVLGR
                     AEVRRPFRISGIGMVAGSYVLEGKVARDASVRVIRDGVEVYEGKVCSLKRFKDDVREV
                     AAGFECGIGVSNYGDLKEKDVLELFRLKEVRRA"
BASE COUNT          798 a         1237 c         1345 g          947 t
ORIGIN      
        1 tagccatggg atcgagagtg ccggcatgac cagctcgtac gccgaacgca cgccgcacgc
       61 gatcgaccac atcgtgcgag gtgactccgg cgggcttgaa aatcgtcagc gccccatcga
      121 gtgaatcagc catcaagctt ctccagttct ggttcgatga cctgcaagag attgtcaatg
      181 gctcgttcca atggtccggc aatctggact cccgcggcga ggcgatgacc accaccaccg
      241 aaggtgcgac tgaccacgca gacgtcgaat cttccggtcg agcgcagact gaccttgatc
      301 tgtccgctct cgagctcgcg aaacagcagg gcgacatccg tgccgacgat agtgcagaga
      361 gggttgatcg ccccatcggc atcgacgttc gttgctccat gtcgcgtaaa atcctcgcgg
      421 gtgagccagg tatactgcag tttgccgcga cagcgcgtcg tgaccctact catggcggtg
      481 ccatgcatca gaatgcgggt gacctgattc gcttcataaa ccaacttgta caaacgcgtc
      541 gcctcgacgc cgcgagtcaa catttggcct gcgatttcgt gagagcgcag ggtggtgttc
      601 ggaaagcgaa agttgccggt gtcggtcatg atgccgacgt agagagcttc caaagacgga
      661 atggtcagct gatggtccat gccctcgagc agatcgaaca gaatctcgca ggtcgatgag
      721 gcctcgtcat caagcacgtt gagatcgccc aaccccttga cgccgacgtg atgatcgaga
      781 tggatgcgcg tggtcgcgcg ggtgaagaac cgggcgcgat cgcccatgcg accgatatcc
      841 gtggtttcca tcaggaagat gacatcgaag tgttcgccat cgtccagacc tcccgcaaaa
      901 tcaccgcacc cggcaagcca cgaaatgctg ggagcgatcg ggtcgaaatt caacgggacc
      961 gatcgtttgc cgagggcgcg gcagaagtga tgcagggcca gttgtgaacc aatcgagtcg
     1021 ccgtcgccgt cggggtgacc gatcgaaagt accgaatgcg ctgaagcgag aacagcacgg
     1081 atcggggcaa acaacgcctc acgttccctg gtgtgcatgc ccgttacttt ccgggctcgc
     1141 cgctttcggg aggagacgcg ggtttgattt ggcgcagcaa ttcgagcaga cgatcaccct
     1201 gacggatgga gggatcgaag aaaaaggcca gctcgggcag aatgcgcagt tgcaacactt
     1261 ttttcaattc ggcgcgaatc gccgacttgg cggagtcgag gcccttctgc atggcttcgg
     1321 ggtctccctc ctgatcgagc atactgtagt taatgcgcgc cagatgcaga tcagggctga
     1381 cgtcgaccga gacgatatgc atgccctgta cgcgcggatc cttgattcgc tggctgataa
     1441 tgcgggcgat ctcatgctgc agcaccgatg cgacgcgctc gaggcgatga tgcttcatac
     1501 atactcccag tggcggttca gcacctgcac ctgatcgtgg tcggtgatga tatcgtcgat
     1561 tttttgtccc atctgctgca gcagggtctg gtcattggat accagagcca cacccagggt
     1621 gcccttctgc cagagatcgt ggtcttcgac ctcggaaatc gaggcgttga acttggcgcg
     1681 cacgcgatcg atcaggcagc gaagaacctg acgcttttcc ttgaggctgt gcagctcagg
     1741 aaagtgcaga tcgtaggtgc cgatcgccac gagcatggcc atgcattatc tccaccaggt
     1801 caggcccggc gaacttcctt caggcggaac agttcgagaa cgtctttttc cttcaggtcg
     1861 ccgtagttcg agacaccgat cccgcactcg aatccggcgg ctacctcgcg cacgtcatcc
     1921 ttgaagcgct tgagagaaca cactttgccc tcgtagactt cgacaccgtc gcggataacc
     1981 cggacggaag catcgcgggc gaccttgcct tcgagcacgt aggaaccggc gaccatgccg
     2041 atgcccgaga tgcggaaggg acggcgcact tcggcgcgac ccagcacttc ttcctcatac
     2101 tccggttcgt acatgccctt caaggccagc ttcacggcat cgatggcgtc atagatgatg
     2161 cggaagacct tgatttcgac gttctctcgc tgtgagactt cgtcgactcc gtgggtcggg
     2221 cgcacatgga agccgatgat gatggcgttc gaagccgcgg ccagcatgac gtcagattcg
     2281 gtgatggcac cgacaccggc gtgaatgacg ttgaccttga tgtccgggtt cgagagacgc
     2341 tcgagagcgt ctttgagcgc gccagaagac ccctgaacgt cggctttgat gatcaggttg
     2401 agttctttca cattgccttc ggcgacgcgg gcgaacaggt tggtcagctt gaggcggttg
     2461 tcgcgttcga ggcgttcttc acgctcctgc ttcagtcgca gcacgccaac gtaccgggcg
     2521 aacttggtgc tctcgacgac gacgagacga tctccgaccc gggggacttc gttgaggccg
     2581 agcacgacca cgggggtcga agggccggct tctttgatac ggctgccggt atcgtcgatc
     2641 aaagccttga cgcggccata gcaggtgccg cagatgatcg agtcgccaac gcgaatcgtg
     2701 cccttgcgga taatcagcgt acccatggga cccatgccct tatcgagctt ggcctcgatg
     2761 atggtgccct gcccctcgcg attcgggttg gccttgagtt ccatgatttc ggcctgcagc
     2821 aggatcatct cgagcagttg gtcgacacca acgccggtct tggcagagac cgggaccatg
     2881 atcgtcttgc cgccccagtc ttcggagacc aggttgtaag gcatgagctg ctgcttggct
     2941 ttctcggcat tggcatcggg cttgtcgacc ttgttcagag cgacaatgat tgggacattc
     3001 gccgcttgcg cgtgatgaat agcttcgacg gtctggggct gcaccccgtc gtcacaggcg
     3061 acgaccagaa tggcgatgtc ggtgatctgc gctccctggg cgcgcatggc ggtaaacgct
     3121 tcatggcccg gggtatcgag gaaggtgatc gatcccgtcg cggatttgac ctgataagcg
     3181 ccaatgtgct gcgtgatgcc gccaatctcg ccttctgcca ctttggcatg acgaatacga
     3241 tcgagcagcg acgtcttgcc atggtcgaca tgacccatga tggtaaccac cggggggcgt
     3301 aacttgagat cggtgtcgac atcaggggtt tcttcgaggt caacgacgtc ctcggaaaag
     3361 gtgatctctt tgccgtattc ggcggcgagc gtctcgacgt gatcgcgctc aagacgctga
     3421 ttcagactgg cgaagacgcc catggtcatg agctttttaa tgatctcgac ggcttcgacc
     3481 tgcagatact tggccagctc gttcaccgtg acttcctggg gaagggtgat gcgcttgacc
     3541 accggttgcg gggcaaacgg cgtgccgggt gcgacctggg cgactgctgc cgatccttcg
     3601 atgaccaggg cttcgccgcg gctttcctcg gtggctttct gcggcaactg gccgcggttc
     3661 ttcattttct tgttcttttt cggcgggccc tctttcttga ctggggcggt tttgatgatg
     3721 gtgatgccac cgtcaccttc ttcgccgatc gggcgaaccg gctttggacc cgtcttcttg
     3781 tcttttgccg gttgtacaat ctgaatgggg gtcggaaaca tcggccgcgg agtctgcgga
     3841 ggcgttgcgg gccgctgggg ctgttgtgcc gtcgcgggaa ccgtctgacc gaccggagtg
     3901 atgggtcggg caggagcctg ctgcggttgt gcgggccgag ttgtctgctg agccggttga
     3961 gttggcgtgg cagggcgatt cgcgggttgc ggggtctggg tgggcttggg ttccaccttg
     4021 gtgatcatcc gcacttcctg tttcggctcg cgcacgacgg gcttggcagg cggcttggtc
     4081 gcggtcggtt cggccttggc tgccgtcggc ttcgcgggcg ccgccgcggg agcggttttt
     4141 ccggctccgc tgaaatgctt ctcgagcgcg gagaccatgt cttcactcac ggcggcaagg
     4201 gcgttgtctt tgacctgaaa accgagcttg gccagaaggg caaccagatc cttccgttcg
     4261 gttttaaatt gcttgactgc ttcgtgtaat cgcatcttcc tacctccgcc gtgacttttg
     4321 cttggct
//
LOCUS       DAORHD010000250         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21824_length_4321_cov_3.216128, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000250 DAORHD010000000
VERSION     DAORHD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21824_length_4321_cov_3.216128"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKO06_06595"
     CDS             complement(<1..347)
                     /locus_tag="PKO06_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV69349.1"
                     /translation="MELLIMLDQLTHPDARAFFSAPDPAAVLVDLHRTGQLPEVLTRL
                     AGIPQPADSHPEGDVWTHTLLVVEQAAMLAREHRLSESQRFLLLLACLVHDLGKMDTT
                     VVGPDGRIRSWKH"
     gene            473..1921
                     /locus_tag="PKO06_06600"
     CDS             473..1921
                     /locus_tag="PKO06_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69350.1"
                     /translation="MRNTIQVLRLMIVSLAMFCLVPAEAQIGSWFGKAVRGLTKSGEK
                     AIAEGVEEGAEQVLKKGSRRSGTALVPNAGRSAGAGKGLTRAGRALSPVQAQAFEQLL
                     KHLGPEAVETATRLAVQYSDDGVRVMNKLGRRGRYYLEAHGPSVSQGLRAATKMGQGE
                     RYLELLARYGDRFINHLDQHKLLYAGGTAFAVFLANPQPFVDSALDVTKKTVDTVVVP
                     TAREVILPVTKEVLQTGSLTTLSSLELVLQTLGGFGLLSILLALLVLRYGVRFARDLI
                     AAMNPRREMPESARQLNRSGPETGEPSSEPSDLDKPPATDTIESTSGAGTSDANMAAA
                     PNEPSPRRSTATGVALVILVSLLVTDVPIQAGVPVVLTPMPPMVGRAKLVKTVIQALW
                     QVRQVTNALRPHAEKAVKWVEKCVLKAPPPVLPNPLITILVEIAVWVVADRTLGGDGP
                     EAPPPPLQWPEALPQTGAWTPPQHVLDQLPEF"
     gene            1989..3233
                     /locus_tag="PKO06_06605"
     CDS             1989..3233
                     /locus_tag="PKO06_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69351.1"
                     /translation="MLIRFTKRFFVMGLVMYSLAALLATSLVGEKAVVRIPVSHERQQ
                     GESIVLADYAEIEGLAWYHTTKDTLAKLPDALRSWLREQIVSLTHQNHVAHLKGEEAI
                     HLTIEHFRRHLDEDVVQAYTTEITSEFDRVRNRIDGFLDWAFSYSTKGWTAVNAMRDA
                     GNWIAECANHVKADLVTVTPNHNAAMFRNEFSSRVFDQSWLDKSMSTICRKYESTLQN
                     KLVMCMQKMRRDLSEILNENMTLPLDSAELDNRTQAALYEMSHEYSLSAGARLVIGTV
                     VSEVLINYTVGSVLATQWYAAASTQAVTWIGLPGSALVAVSTGLVRGIVGVIGFVVVD
                     YFIDSWQRPRLREQLISMISKLENGLIYGAGTSDGFRISARNLNAEVCRTLESLFQQK
                     ILEKAIENRVAACKLLQSLIGS"
     gene            3340..4242
                     /locus_tag="PKO06_06610"
     CDS             3340..4242
                     /locus_tag="PKO06_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV69352.1"
                     /translation="MTRQDILQQLLNSWKNEVRHPDLAGLRRRLSSLTDRLEAVGAET
                     DDVVRIVNAGLLKAGKSLLFSALSGRRETFAVGAARCTLKSATCSFGDALLCDTPGID
                     AYDEDQREAEKALQNAHLIVFVHSMSQGELQAPEITFLTTLRAMVQSNGPGDLRIVPV
                     LTRTDEVGERKEQERLVRRLQEQWRETLNEPSPEWFRTSATRFMKSLATGDQRWAELS
                     GVPRLRAYLEHRVPELRSLIPCLRRRRMQEIIDAMAIPVKAAEQERVRIALAKKRAAA
                     EKAQRQRRHLKELMESIEARRSSI"
BASE COUNT          962 a         1153 c         1311 g          895 t
ORIGIN      
        1 tcgtgcttcc aggaccgaat gcgcccgtca ggaccgacga cggtagtgtc catcttgccg
       61 aggtcgtgaa ccaggcatgc caaaagcaaa agaaatcgct gcgactcgga caaacgatgc
      121 tctcgcgcca gcatcgccgc ctgttcgacg accaggaggg tgtgggtcca gacgtcgcct
      181 tcggggtgcg agtcggcagg ctgggggatg cccgcgaggc gggtgagtac ctcgggcagt
      241 tgaccggttc ggtgcagatc gacgagaacc gccgccgggt ccggcgctga aaaaaaggcc
      301 cgggcgtccg ggtgtgtgag ttggtcgagc atgatcagca gttccatacg tacctccgtt
      361 ataccatgta acgaaaagaa agcgccttct gtcggcacgg ttcacttttt gaacccgttt
      421 cgcggtggca ccttgtacga ggtaaccaaa atcgaaaaac ggaggcgcga cgatgagaaa
      481 caccattcag gttcttcgat tgatgatcgt ttccctggcg atgttctgcc tggtgccggc
      541 tgaggcgcag atcggcagtt ggttcggcaa ggccgtgcgc ggactcacga aaagcggtga
      601 gaaagcgatc gccgaagggg ttgaggaagg ggcagaacag gtgctgaaga aaggctctcg
      661 acgctccgga acggccctgg tacccaatgc tggccgctcg gccggcgcgg gcaagggtct
      721 gacgcgcgcg ggccgggcgt tgtcgccggt tcaggcgcag gcattcgaac aattactgaa
      781 gcatctcggt cccgaggccg tcgaaacggc gactcgtttg gccgtgcagt acagtgatga
      841 tggggttcga gtcatgaaca aactcggacg tcgaggtcgc tactacctcg aagcgcacgg
      901 tccgtctgtc tcacagggcc tgcgagcggc cacgaagatg ggacagggcg aacgctatct
      961 cgagctgttg gcgagatacg gcgatcgttt catcaatcat ctcgatcagc acaaactgct
     1021 ctatgccggc ggtacggcgt tcgccgtgtt tctcgccaac cctcagccgt ttgtcgatag
     1081 cgcgctcgac gtgacgaaga agaccgtcga caccgtcgtt gtgccaactg ctcgcgaagt
     1141 gattctgccc gtcacgaaag aagttctgca aaccggcagt ctaacgacgt tgagcagtct
     1201 tgaactcgta ctgcagacgc tcggtggttt cggattgctg tcgattctgc ttgccctgtt
     1261 ggttctgcgc tacggggtca ggtttgcccg cgatctcatt gccgctatga accctcgcag
     1321 agaaatgcct gaaagcgccc ggcagttgaa tcggtccggg cctgagaccg gtgagccctc
     1381 aagcgaaccg tcagacctcg ataagccgcc ggcgacggat acgatcgagt cgacttcggg
     1441 cgctgggacg agcgacgcga atatggccgc ggcgcccaac gaaccatcgc cccgccgttc
     1501 gacggcgacg ggagtcgctt tggtcattct cgtcagtctt ttggttacag atgtgccgat
     1561 ccaggccggt gtcccggtag tgctcactcc aatgcctccg atggtgggca gagcaaagct
     1621 ggtgaaaacg gtcattcaag ccttgtggca ggtgcgccag gtaacaaatg ctctgcgccc
     1681 gcacgcagaa aaggccgtga aatgggtgga aaaatgcgtg ttaaaagctc cgccacctgt
     1741 gcttccgaat ccgctgatca ccattcttgt cgagattgcg gtatgggttg tagcagatcg
     1801 aactctcggc ggcgacgggc ctgaagcgcc cccgcctccg ttgcagtggc ctgaagcact
     1861 accgcaaacg ggggcctgga cgccgcccca gcatgtgctc gatcagcttc cggaattcta
     1921 aaccaagaga tgtgttctgc cttcatctaa gtgacataac caaaacacgg agtttgagga
     1981 ggatgtgtat gttgattcgt ttcaccaaac gctttttcgt catgggcctg gtcatgtaca
     2041 gtctggcggc gcttttggcg acgtcccttg tcggtgaaaa agccgttgtg agaatacctg
     2101 tttcgcatga gcgacaacag ggagagtcaa ttgtactagc tgattatgct gaaatcgaag
     2161 gtcttgcctg gtatcacaca acgaaagata cactcgcgaa attgcctgat gctctgcgtt
     2221 cctggttgcg tgaacagatc gtatccctga cgcaccagaa tcatgtggcc cacctgaagg
     2281 gcgaagaagc cattcatctg accatcgaac acttccgccg acacctcgat gaagatgtcg
     2341 tccaagccta cacgaccgaa atcacgagtg aattcgatcg tgtcagaaac cgtatcgatg
     2401 gatttcttga ctgggcgttt tcctattcga ccaagggctg gaccgcggtc aatgcgatgc
     2461 gggatgccgg gaactggatc gcggagtgcg ccaatcatgt caaggccgat ctcgtcaccg
     2521 tgacgccgaa tcacaatgcg gcgatgtttc gcaacgagtt cagcagccgc gtattcgatc
     2581 aatcgtggct tgacaaatcc atgagcacga tctgtcgaaa gtacgagtca actctgcaga
     2641 acaagcttgt catgtgcatg caaaagatga ggcgagacct atcagaaatt ctgaatgaaa
     2701 acatgactct gccgctcgac agtgcggaac tcgataaccg gactcaggca gcgctatacg
     2761 aaatgtcgca tgaatactct ttgtcagccg gtgctcgtct ggtgattggt actgttgtca
     2821 gtgaagttct gatcaactat accgtgggaa gcgttctcgc cacgcagtgg tatgccgcgg
     2881 catcgacaca agccgtcacg tggatcggct tgcctggctc agcgctggtt gcggtcagta
     2941 cgggtctggt gcgtggaatc gtcggagtga tcgggtttgt cgtcgtggac tacttcatcg
     3001 acagttggca gcgaccgaga ttgcgtgaac agttgatcag catgatctcg aagctggaaa
     3061 acggtctcat ttacggtgcc ggcacaagcg acggatttcg aatctccgca cggaacctga
     3121 acgccgaggt ctgtcgtacc ctcgaaagcc tgttccagca gaagattctg gaaaaggcga
     3181 tcgaaaatcg agtggctgcc tgcaagctgc tgcagagcct gatcggaagt tgacggagtt
     3241 tcgacgcgat tcgcagattt tgaaccgtga gggcggaacg agctttgcat aagtgtcggg
     3301 tggtgcgaaa tcagcaacag aaatacaacg gagggcgtta tgacacggca ggacattctc
     3361 caacaactgt tgaacagctg gaaaaacgag gttcggcacc ccgacctggc gggtttgaga
     3421 cgtcgccttt catctctcac cgaccgtctc gaagctgtcg gtgccgagac ggatgacgtg
     3481 gtgcgtatcg tcaatgccgg cctgttgaaa gcaggaaaaa gtctgttgtt ctcagcgctc
     3541 tcgggtcgac gggagacctt cgccgtcggt gcggcccggt gcacgttgaa aagcgcgacc
     3601 tgttcgttcg gtgacgcgct tctgtgcgat acgccgggga tcgacgcgta tgacgaagac
     3661 cagcgcgagg cggaaaaagc tctgcagaat gcgcatctca tcgtgttcgt tcacagtatg
     3721 agccagggag aactccaggc cccggaaatc acttttctca caacattgcg agccatggtt
     3781 cagagtaacg ggcccggtga cctccgaatt gtaccggtac ttacacgtac agacgaagtc
     3841 ggtgagcgga aagagcagga acgactggtt cgccgattgc aggaacagtg gcgggagact
     3901 ctgaatgaac cttcgccgga atggttccgc acctccgcaa cgcgtttcat gaaaagtctc
     3961 gcgaccggcg accagcgctg ggcggaattg agcggagttc cgcgactgcg ggcctacctc
     4021 gaacatcggg ttcctgaact gcgctcgctg attccctgtc tgcgccgtcg gagaatgcag
     4081 gagattatcg atgcgatggc gattccggta aaggcggctg aacaagaacg ggtgcgcatc
     4141 gcgctggcaa agaagcgggc agccgcggag aaagcgcaga gacaacgacg gcacctgaag
     4201 gaactgatgg aatcgatcga agcgcggcgg tcctcaattt aggccgcgac tgaagaatct
     4261 ctctgaaatg ccatgaagga ggcaatctat gttttggttc atccccgttg cccttgttgg
     4321 a
//
LOCUS       DAORHD010000251         4316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21857_length_4316_cov_3.909880, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000251 DAORHD010000000
VERSION     DAORHD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21857_length_4316_cov_3.909880"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            343..1377
                     /locus_tag="PKO06_06615"
     CDS             343..1377
                     /locus_tag="PKO06_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69353.1"
                     /translation="MRTLMVSLLAIILLPANILNAQVKFTWMKDKPGKWQYNYRINTS
                     SAEEIRFKKQVEELSEWFHQNVPLFKKPMGFDVKVHVSRSESERQAKSPAHDALSANL
                     SFNLELFARDGKPWKAELPSASYYNYPCLNRIAGSGSWLGSIGQFDSFNNTKHDPKLE
                     DAINRAASKLDEMMTVFPRERELAPGLHLYKCQARGTFVVVFRPDRPAYLIPVTLREL
                     AEACTNYYSLFQTLEIDRMLLKELKKELAAFSETDLNRPAYAGHPSNVVFPYSSDSRN
                     LPIMKMNPDYWDRSLPSSAIQLMVFWDPGFSEQEMTAHLERFGYPIHSQILVNQINWN
                     DMVKFVARKQ"
     gene            complement(1520..2434)
                     /locus_tag="PKO06_06620"
     CDS             complement(1520..2434)
                     /locus_tag="PKO06_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69354.1"
                     /translation="MIIRHQSVRGTCLFCGMKTGHLHLPDIGAEEHFEVAPDGTIRYE
                     DPVYRECPRARWILGLLYYGLFLPFIFLTGGWAPAVFMLLLGLVGTALLFPPSESKWV
                     GLSEVKAEYDSLVQELKASKRTYYLRSLEESLVPLFKLRDRALNMVRALADMPTVMIE
                     NQLDVIRARSVDENDAELVEIYRAQIRDLYQLVQRREQMQRFLNRFEASKKSVAASIN
                     LLKSKILTSEQAGTGGDERRIMDDLKMLHSIYERVNESGPKSETGRNEQQDPKSSALG
                     ALSEPDPESEISEELRRRLENLPSRERR"
     gene            complement(2465..3592)
                     /gene="alr"
                     /locus_tag="PKO06_06625"
     CDS             complement(2465..3592)
                     /gene="alr"
                     /locus_tag="PKO06_06625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005036207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV69355.1"
                     /translation="MLYQTHARIHLGNIRHNLEQIRAAVGPDKKILIAVKANAYGHGA
                     VELSRMAERIGIDWLGVATVPECLELRKAGIRLPILKFSPAFSDEMQAAVEHGLTLAV
                     CEKKNIIELNEICSRIGKKANVHLKVDTGMGRIGCLPEEAPDLAMFISAECPFLFLEG
                     IFTHLPVSDEASKTYTNRQLQLFRKTVESCEKRISGKIPLAHCANSGGVLAYPESWLS
                     MVRPGIMIYGFYPAKETPQTIPLRPGLSFLTRVSFVKKVTKGTSIGYGRSWVAPDDTF
                     IATIPAGYADGFNRLFSNMGRVLIKGKSYPIVGRVCMDQSMVNLGGKTEVQVGDEVVL
                     IGRSGNEEITVNEWAEKLETITYEVTCQINQRVQRIFEPDL"
     gene            complement(3595..>4316)
                     /locus_tag="PKO06_06630"
     CDS             complement(3595..>4316)
                     /locus_tag="PKO06_06630"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV69356.1"
                     /translation="KVIIMVDVGDLREGIWPDRAPEVVAKAKELRGIEVVGLGCNLAC
                     YGGVIPSVENMQLLVKTRDECRRATGLELPILSGGNSSGLPLLASGKMPREINHFRVG
                     EAIVLGRNVIDRSPWPGTRQDTFVGVGEVIEVEKKPSLPIGDRGQDAFGESQTFVDRG
                     WRHRAIINLGRQDVKVDGIEPIDPKIIVLGGSSDHLLLDVEEAAVMPKVGSEFSFYPG
                     YGALLALTTSAYVHKTVMPGE"
BASE COUNT          972 a         1252 c         1131 g          961 t
ORIGIN      
        1 gaagggaaag ggtgtgcagt ataaaggggc tcgaccgcaa acacaatcca tttgtcggtt
       61 ccctgactca agcccgggac cctttctggc aaacggaaac cgggaagcag atcgttttca
      121 cacaccgcgt tccggaggtc tgttcggggg atccggcatc tcggccagcg aaacaaacga
      181 gacaggatag ccttgacaag tggaatgcgc ccctctaagc tgcaacgccg agagagccgg
      241 agaatcggga acccaacgaa tcgcgctaca actcacactc tcgttgtttt tcactctctc
      301 tccctcaacg acgatactct tattcacagg ggacgacatt tcatgagaac gctgatggtg
      361 agcttgctgg caataattct tctacccgcg aacatcttga atgcccaggt gaaattcacg
      421 tggatgaagg ataaacccgg aaagtggcag tataactacc gcatcaacac gtcgtctgct
      481 gaggaaattc ggttcaagaa acaggtcgag gaattgtccg aatggtttca tcagaatgtg
      541 cctcttttca agaagccaat ggggtttgac gtgaaggtgc acgtttcaag aagtgaatct
      601 gaacggcaag caaagtcgcc tgctcacgac gccttgtcag caaatctgtc gttcaacctt
      661 gaactgtttg ccagggatgg caaaccgtgg aaggccgaac tgccatccgc gagttactac
      721 aattatcctt gcctgaaccg catcgccggc agtggttcgt ggcttggttc gattgggcag
      781 tttgactctt ttaacaacac caaacacgat ccaaagcttg aagacgccat caatagagcg
      841 gcttctaagc ttgatgaaat gatgaccgtg tttccacgag agagagaact ggctcccggc
      901 ctgcatctct acaaatgtca ggcccgagga acattcgtcg ttgtcttccg cccggaccgc
      961 cccgcctatc tcattccggt cacgttgcga gagcttgcag aagcctgcac gaattactac
     1021 tctctttttc aaacgctcga gatcgacaga atgctgctga aagaattgaa aaaagaactt
     1081 gcggcgtttt cagaaacgga cctgaatcgc ccagcctacg ccggtcatcc ctccaacgtt
     1141 gtctttccct attcttccga cagccgtaat ctgccgatca tgaaaatgaa ccccgattac
     1201 tgggaccgct ctctgccttc ttcggcgatt caactcatgg tcttctggga cccggggttt
     1261 tctgaacagg aaatgaccgc acatctcgag agattcggtt accccattca ctctcaaatt
     1321 ctggtcaacc agatcaactg gaacgacatg gtcaaattcg ttgcccggaa gcaatagaag
     1381 ccacctttcg gcactctgaa ggaacccgtc gtcacgaaca gtgtggtcac gggaaacggg
     1441 atgtctgcag agaagactga accccggttg tttcgcagac aaggtgagaa aatgccgcga
     1501 cagatccact tgtgcgcggt cagcgtctct cccgcgaagg cagattttcg aggcggcggc
     1561 gcagttcttc cgatatttcc gattccgggt cgggctcaga cagggcgccc agtgcggaac
     1621 tcttcggatc ctgctgttcg tttctccccg tttctgactt cgggcccgat tcattcacac
     1681 gctcgtagat agaatgcagc atcttcagat cgtccatgat gcgccgctcg tcgccgcccg
     1741 tgcctgcttg ttccgaggtc agaatcttgc tcttgagcag attgatcgat gccgcgacac
     1801 tttttttgct ggcttcgaac cgattcagaa atcgctgcat ctgttcgcgc ctttgtacca
     1861 gttgatacaa gtcgcgaatc tgtgcccgat agatttcgac caactcggca tcgttctcgt
     1921 caaccgaacg ggcccgaatc acatcgagct gattttcgat catcaccgtc ggcatgtcgg
     1981 ccagggcgcg caccatgttc agcgcgcggt cacgcaactt gaacaagggt acaagtgact
     2041 cttcgagtga acgcaggtaa taggtgcgtt ttgaagcctt cagttcttga accaggctgt
     2101 catactccgc ctttacctca ctcaaaccga cccacttgct ttctgacggc ggaaacagca
     2161 gtgcggtacc gacgagtccg aggagcaaca tgaacacagc cggagcccat ccgcctgtga
     2221 gaaaaatgaa aggcagaaac aggccataat acaagagacc gagaatccag cgagcccgcg
     2281 gacattctcg atagaccggg tcttcgtagc gtatggttcc gtccggggcc acttcgaaat
     2341 gttcctcagc cccgatatcg ggaagatgca gatgtccggt cttcatgccg caaaagaggc
     2401 aggttccacg cacgctctgg tggcggataa tcacatcatc ttccctggac gcacgatgat
     2461 tcgattacag atcgggctca aagatgcgtt gcacgcgctg gttgatctga caggtcacct
     2521 cataggtaat ggtttccagt ttctcggccc actcgttcac ggtaatctcc tcgtttccgg
     2581 aacggccgat caacacgacc tcgtcgccta cctgcacctc ggtcttgccg cccaggttca
     2641 ccatcgattg atccatgcag acgcggccga caatcgggta cgattttccc ttgatcagaa
     2701 cccggcccat gttcgagaac aggcgattga acccatcggc ataccccgcg ggaatggtgg
     2761 cgatgaaggt gtcgtcaggt gccacccagc ttcgcccgta tccgatcgag gttcccttgg
     2821 ttactttctt gacgaacgag acgcgcgtca aaaacgacag ccccgggcgc agcggaatgg
     2881 tctgcggtgt ttctttcgcg ggatagaatc cgtagatcat gatgcccggt cgcaccatgc
     2941 tgagccagct ttccgggtag gccagcactc ccccgctatt ggcacagtgc gccagcggaa
     3001 tcttcccgga aatgcgtttt tcgcaactct cgaccgtttt acgaaacagc tgcagctgcc
     3061 gattggtata cgtttttgag gcttcgtcac tgaccggtag atgcgtgaaa atgccttcga
     3121 ggaacaggaa cgggcactcg gcggaaatga acatcgccaa atcaggggct tcttccggta
     3181 gacagccgat tcggcccatt cccgtgtcaa ccttcagatg cacattcgct ttcttcccga
     3241 tgcggctgca gatctcattc agttcgatga tgttcttttt ctcgcagacg gccagcgtca
     3301 acccgtgttc gaccgcggcc tgcatctcat cggaaaacgc cggactgaac ttgagaatcg
     3361 gcagtcgaat tccggccttg cgcagttcga gacactcagg aaccgtggcc acgcccagcc
     3421 agtcgatgcc gatgcgctcg gccatgcgac tgagttcgac cgcgccgtga ccataggcat
     3481 tggctttgac ggcaatgaga atcttcttat cggggccaac ggcagcgcga atctgctcga
     3541 ggttgtggcg aatgtttccc agatgaatgc gggcgtgtgt ctgatacagc atgattactc
     3601 tccgggcatg acggttttgt gaacataggc agacgtggtc agcgccagca gggctccata
     3661 ccccgggtaa aaactgaact cactgcccac ctttggcatc acggcggctt cttcgacatc
     3721 gagaagcaga tgatcgctgg aaccacccag aacgatgatc ttcgggtcga tcggttcgat
     3781 gccgtcaacc ttcacgtcct gccggccgag attgatgatt gcgcgatgac gccagccacg
     3841 atcgacgaag gtctggctct caccgaatgc gtcctgtcca cgatcgccga tgggcaggga
     3901 aggcttcttc tcgacctcga tgacctcgcc aaccccgaca aacgtgtcct gacgcgtgcc
     3961 cggccaggga gaccggtcga tcacatttcg ccccagcacg atggcttcgc cgacacggaa
     4021 gtggttgatc tcgcgcggca tcttccctga ggccagtaat ggaagccctg agctgttgcc
     4081 cccgctcaga atcggcagtt ccagcccggt cgcgcgacgg cattcatcgc gggtcttgac
     4141 cagcagttgc atgttctcga ccgacggaat gaccccgccg taacaggcca gattgcagcc
     4201 cagaccaaca acttcgatac cgcgcagttc tttggctttc gcgaccactt cgggagcacg
     4261 gtcaggccag atgccttcgc gcagatcgcc gacatcgacc atgatgatga ccttgt
//
LOCUS       DAORHD010000252         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21895_length_4312_cov_3.197557, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000252 DAORHD010000000
VERSION     DAORHD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21895_length_4312_cov_3.197557"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKO06_06635"
     CDS             <1..468
                     /locus_tag="PKO06_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV69357.1"
                     /translation="RSVPDDDACNSSDFFDGYGTLYGTVRQLLPQEALELIKKGAVLV
                     DVRDEAEMNGKYFQIPGLIYLERRHLEDRFERLPKDKPLILADSVGMKGKEAVVFLQQ
                     KGYTQLANINGGIFAWEDEKLPMVVKEEEILAGECACRLRPRKAYRSKSGTGC"
     gene            493..1701
                     /gene="arsB"
                     /locus_tag="PKO06_06640"
     CDS             493..1701
                     /gene="arsB"
                     /locus_tag="PKO06_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNV69358.1"
                     /translation="MTVTEACVSKEPGGLGFFERYLTLWVVLCIIAGIVLGKLAPGVA
                     TYLDSLSITVDGAPVVSIPIAICLFFMMYPIMVKIDFAEVLKAGKSIKPVGLTLIINW
                     AIKPFTMYAIAIIFLGTLFLGFIGPDATDLVKMPLGLDLPVDAVHGEGKVVLHNGVKM
                     LQVPLWRSYLAGCILLGIAPCTAMVLVWGFLARGNAGHTLVMVAINSLTMLLLYGPLG
                     GFLLGIGKLPVPWQALLLSIGIYVALPLIAGYLSRRWIISAKGEEWFKETFLHVLTPI
                     TIVALLLTLVLLFSFKGETILSNPMTILWIAIPLFIQTMLIFWLGYALSKPLGLTYED
                     AAPSAMIGASNHFEVAIATATMLFGLQSGAALATVVGVLIEVPVMLWLVRICRQTTHW
                     FPRQSDSTPS"
     gene            1742..2602
                     /locus_tag="PKO06_06645"
     CDS             1742..2602
                     /locus_tag="PKO06_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HNV69359.1"
                     /translation="MKKTQKPVDPQKIYEAVRSGYAKVATGQSGSCCGPASSCCGGGK
                     PADAEQLARVIGYSDDDLKALPEGANMGLSCGNPSAIAALAPGEIVLDLGSGGGFDVF
                     VVARKIGPTGRAIGVDMTPEMLTKARRNAIQFAKQTNLDNVEFRLGEIEHLPVADNSV
                     DVIISNCVINLSPDKPQVWHEMFRVLKPGGRVAVTDLALRRPLPEALRDSVAALVGCV
                     AGAVLIEETRSMAMAAGFGQIDINDKSAYLESMTSANDPLYREIIDQLPPNTHLDDFV
                     ASISISARKP"
     gene            complement(2626..4092)
                     /locus_tag="PKO06_06650"
     CDS             complement(2626..4092)
                     /locus_tag="PKO06_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNV69360.1"
                     /translation="MNWKEEWKTLLAMIAVFLGCYFLPIGTPRFDNAVMEALHLVKWY
                     AREHVLLCLVPAFFIAGAITIFISQASVMKYLGPNANKMLAYGVASVSGTILAVCSCT
                     VLPLFNGIYKMGAGLGPACAFLYSGPGINVLAIVMSARILGMDIGIARAIGAISFSVI
                     IGLGMHLLFRAEEEEKALAAAQFPEPENVKPLWHNVVFFGAMIGILVFANWGKPQSVV
                     LTLKDGAVIAGEVQEEIEGFPTITLKVDSASGRPWASGTAQVVPLNEITQRTIPDTPW
                     KTIWSNKWKVTAGFAVALLLILGTLLKMAWWRVGVTGAAVLIGYLAAPTDPLVPFVTM
                     TIALSVMTSTSTDDSKDWFASSWDNAKMILPLLLGGVLVAGFLLGRVGHEGAIPSEWV
                     KSLVGGNSLFANFFASIFGAFMYFATLTEVPIVQGLIGSGMGKGPALALLLAGPALSL
                     PSMLVINQLMGFKKTFAFVCLVVILSTFSGMIFGALVG"
BASE COUNT          935 a         1292 c         1206 g          879 t
ORIGIN      
        1 cgatcagttc cagatgacga tgcctgcaat tcttcagact ttttcgatgg gtacggaacc
       61 ctgtatggaa ccgtgcggca gctgctgccg caggaagctc tcgaactgat caagaagggg
      121 gccgttctcg tcgatgtacg tgacgaagcc gagatgaacg ggaagtactt ccagattccc
      181 ggtctcatct acctcgagcg ccggcatctc gaagaccgtt tcgaacgtct gcccaaagac
      241 aaaccactga ttctggccga ctcggtgggc atgaagggca aggaagccgt cgtgtttctg
      301 caacagaagg gctacaccca gctcgccaac atcaacggcg gcatctttgc gtgggaagat
      361 gaaaagctcc ccatggtcgt caaggaggaa gagattctcg cgggagagtg cgcctgccgg
      421 cttcgcccgc gcaaagcgta ccggtcgaaa agtggtaccg gttgctgacc atcaacgaat
      481 ggaggaacac caatgacggt gactgaggca tgtgtgagca aggaaccagg cggactgggt
      541 ttctttgaac ggtatctgac tttgtgggtc gtgctttgca tcattgccgg catcgttctc
      601 ggcaagctcg cacccggggt cgcgacctac ctcgacagcc tgtctatcac ggttgacgga
      661 gctcctgtcg tgtcgattcc catcgccatc tgtctctttt tcatgatgta tcccatcatg
      721 gtcaagatcg atttcgccga ggtgctgaag gctggcaaga gcatcaaacc cgttggattg
      781 acgctgatca tcaactgggc gatcaaaccg ttcaccatgt atgccatcgc gatcatcttt
      841 ctcggcacgc tgtttctcgg cttcatcggc ccggacgcca ccgacctggt aaagatgccg
      901 ctcggtctcg acctgcctgt cgatgccgtt cacggggaag gcaaggtcgt actgcacaac
      961 ggtgtgaaga tgttgcaggt accgttgtgg cgcagctacc tggcgggctg tattctgctc
     1021 gggatcgccc cctgcacggc catggtgctc gtctggggtt ttctcgctcg tggcaatgcc
     1081 ggtcacaccc tcgtcatggt cgcgatcaac tccctcacca tgctgttgct gtatgggcca
     1141 ctcggcggct ttctgctggg gatcgggaag ctcccagtcc cttggcaggc cctgctcttg
     1201 tcaatcggca tctacgtggc gctgccgttg attgcgggct atctctcgcg ccgctggatc
     1261 atttccgcca aaggggaaga gtggttcaaa gagacgtttt tgcatgtgct gacgccgatt
     1321 accatcgttg ccctgttgct cacccttgtc ctgttgttct cgttcaaagg ggaaaccatt
     1381 ctcagcaatc cgatgacgat cctctggatc gcgattcccc tgttcattca gacgatgttg
     1441 atcttctggc tcggatacgc gctgtcgaag cccctcgggc tcacctacga agacgccgcg
     1501 ccctcagcca tgatcggtgc atcgaaccat ttcgaggtcg cgatcgccac ggcgaccatg
     1561 ttgttcggcc tgcagtccgg agccgctctc gccacggttg tcggcgttct gatcgaagtg
     1621 cccgtcatgc tctggctggt ccgtatctgc cggcagacca cccactggtt tccgcgacaa
     1681 tctgactcaa cgccatcttg aggcggaaac ctgcaccccg tattcatcag aaaggcaacc
     1741 tatgaaaaag actcagaaac ccgtagaccc tcagaagatc tatgaagcgg ttcgttccgg
     1801 atacgcgaaa gtcgcaactg gtcagagcgg ctcgtgttgc ggcccagcgt cttcatgctg
     1861 tggcggcgga aagccggctg atgcggagca gctggccagg gtcatcggtt attccgacga
     1921 tgatctcaaa gcgctccccg agggcgcgaa catgggtttg tcgtgtggga acccatctgc
     1981 cattgcggca ctcgcacctg gagagatagt actcgacctc ggcagcggcg gcggtttcga
     2041 cgtcttcgtc gtcgcgcgca agatcggccc gacggggcga gccatcggcg tcgacatgac
     2101 tccggagatg ctgaccaagg cgagacggaa cgccatacag tttgcgaagc agaccaatct
     2161 ggacaacgtc gaattccggc tgggcgagat cgagcatctc cccgtggccg acaacagcgt
     2221 tgacgtcatc atctcgaact gtgtcatcaa cctctcgcct gacaaacccc aggtctggca
     2281 cgagatgttt cgcgtgctga agccgggtgg ccgtgtcgcc gttaccgatc tcgctctgcg
     2341 tcgccctttg cccgaagctc tgcgcgactc ggtggccgcg ctggtcggct gcgtggccgg
     2401 agccgtgttg atcgaagaga cccgctcgat ggccatggcc gccggatttg ggcagatcga
     2461 catcaacgac aagtccgcgt atctcgaatc catgaccagc gccaatgacc cgctctatcg
     2521 cgagatcatc gatcaactgc cgcccaacac ccacctcgac gacttcgtcg ccagcatcag
     2581 catctcagca cgcaaaccgt agctttgcga tttgtaccat cttcatcaac cgacaagcgc
     2641 gccgaagatc atgccggaaa aggtcgagag aatgacaacc aggcagacaa aggcaaacgt
     2701 cttcttgaag cccatcagtt gattgatgac cagcatgctc ggcagcgaca gggcggggcc
     2761 cgcgagcagc agggccaatg ccggcccttt ccccatgccg ctgccgatca ggccttgaac
     2821 aatggggact tccgtgaggg tggcgaagta catgaatgcg ccgaagatag aggcgaagaa
     2881 attcgcaaag agcgagttcc cgccgaccag ggactttacc cactcgctgg gaatcgcgcc
     2941 ttcgtgcccg acgcgaccga gcagaaagcc tgccaccagc actccgccca gcagtagcgg
     3001 cagaatcatc ttggcgttgt cccacgacga ggcgaaccag tctttcgagt cgtcggttga
     3061 tgtcgaggtc atgacactca gcgcgatcgt catggtaaca aacggtacca gcggatcggt
     3121 cggcgctgcc agataaccaa tgagaaccgc ggcaccagtg acgccaacac gccaccaggc
     3181 catcttcaga agagtaccga gaatcagtag cagtgctact gcgaaaccgg cggtcacctt
     3241 ccacttgttc gaccagatgg tcttccaggg ggtgtccgga atcgttcgct gagtaatttc
     3301 gttcaacggt acgacttgcg ccgttcccga agcccagggg cggccggacg cggaatcaac
     3361 cttcaacgtg atggttggga agccttcgat ctcttcctga acctcgccgg caatcaccgc
     3421 gccgtctttc aacgtcagaa cgacactctg cggctttccc cagttggcaa agacaagaat
     3481 tccgatcatc gccccgaaga agaccacgtt gtgccagagg ggtttcacgt tctcgggttc
     3541 gggaaactgc gccgcggcca gggccttctc ttcttcctcc gcgcgaaaca gcagatgcat
     3601 gcccaaaccg atgatcacgc tgaacgagat cgcgccgatc gcgcgagcga tcccgatatc
     3661 catgcccagg atgcgagcgc tcatgacgat ggccagcacg ttgatgccgg ggccggagta
     3721 caagaacgcg cacgcgggac caagcccggc gcccatctta tagatgccgt taaacaacgg
     3781 aagaacggtg caggaacaga cggcgagaat ggttcccgag acagaagcca caccatacgc
     3841 gagcatcttg ttggcgttgg gtccgagata cttcatgact gaagcctgac tgatgaagat
     3901 ggtgatggcc ccggcaatga agaaggcggg aaccaggcac agcaacacgt gttcgcgggc
     3961 ataccacttc accagatgca gggcttccat gacggcgttg tcgaaacgcg gcgtgccgat
     4021 cggcagaaag taacacccaa gaaacacggc gatcatcgcc agcaacgttt tccattcttc
     4081 tttccagttc atgaccagac cccttgcatg ttcacgagaa agtgacaaca accaaatacc
     4141 gagttcagta tcaaccgaac ccttgtcaac aataggtttc tatacgtcgg ttctcggact
     4201 cttcagaaaa ctccgactat ccgcgcagga aaaacatgcc gactccgaga atcaatactc
     4261 cggcgatctt cttcacgatc gcgacaaaac ggttcatccc tttcgactca gc
//
LOCUS       DAORHD010000253         4310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21904_length_4310_cov_3.352761, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000253 DAORHD010000000
VERSION     DAORHD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21904_length_4310_cov_3.352761"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="PKO06_06655"
     CDS             <1..1044
                     /locus_tag="PKO06_06655"
                     /inference="COORDINATES: protein motif:HMM:NF010589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HNV69361.1"
                     /translation="PHEQAKGGVRPNLIARYRGKSQKKTIWIMSHLDIVPPGDLSKWD
                     SDPYKLRIDGDRLYGRGTEDNHQGLVASLLCVKAMMDCGHRPAYDIGLLFAADEETGS
                     AYGAGYLVEKHPTLFGKDDMFIVPDGGYPDASLVEVAEKSIWWLKIRATGKQCHASMP
                     HQGKNAFRAASALVVKLNSLYQTFARENPLFDPPISTFEPTKKEANVPNINTIPGDDV
                     FYLDSRVLPEYPLSEVDKEIRRLAAEVEKEYGVSFAFETIQQGNAAPPTSPDAEIVTG
                     IIGGIKKVWGITPSPKGIGGGTVAAFFRTLQLPAVVYSKQDEVAHQPNEYCILSNLIG
                     DAQVFAHTILHLK"
     gene            1097..2095
                     /locus_tag="PKO06_06660"
     CDS             1097..2095
                     /locus_tag="PKO06_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV69362.1"
                     /translation="MKINCHCHIFSLDCVPLGFRREFALDLQHPGHRLLHSTLHKLLK
                     DQFKLDTWLKFAEFSIGEIADRLIDEMDEAGIEAATPLSVDLEFCEKYGGTTKSFLQQ
                     IDETLAAVQATTERCGRQRLFPFIGADPRREGIADLILAHLQARRFFGVKIYPVMGFL
                     PDDERLQPVYDYCERQRVPVTAHCQNGGLPGFTGYYDTAHPNNWRPVLERFPKLVLNL
                     AHNDTTGSTWQPPLFQLMRDFPHVYTDVSFDTEMFYQPGTYFRSIKSLLNDKQLCERV
                     LYGTDWYMGRFLWTEKTYLDWFTRFSRKIFWDSVQFTTAEIRRLVEENPARFLGWS"
     gene            2099..3412
                     /locus_tag="PKO06_06665"
     CDS             2099..3412
                     /locus_tag="PKO06_06665"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69363.1"
                     /translation="MTDSSTHIPDPGNIPPAEPALNETALSPAGATFSTLWKKLQAIG
                     TLPEDSPDERTRKTVLTLMAFLSIAIGLLLGLFDVLTGFYYGAVIPTLYAAVSAIGLV
                     HFSRTRNLRFIRLLVLGGLLICPFCAMWLRGGFANSGASMIWALGAPVFGVMFHGQAR
                     SIRWFVAYLALLLLSVLIDPACARFAIEHQIAPSSWYFLAHIGGISFCLFVLIQFFVQ
                     RLGEEQAKSESLLLNILPRQVAGRLKHSSATIADGFQEATILFADLAGFTRLSSSLAP
                     GQVVHLLNEVFSAFDRLAEKHGLEKVKTIGDAYMVVGGIPEPAPDHPHAVARMALEMR
                     ETVAELNRQHNWELRLRIGINTGPVVAGVIGLKKFIYDLWGDAVNIASRMESTGIENE
                     IQVTQSTYERLHPAFTLEPRGKVQVKGKGEMETWLLKSVAAVGQT"
     gene            3594..>4310
                     /locus_tag="PKO06_06670"
     CDS             3594..>4310
                     /locus_tag="PKO06_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV69364.1"
                     /translation="MAEIATRRLFIALFLFAVWFGVSVASAATPERGVSLTILHTSDI
                     HARLLPYDCASGTAMGGYARIKAYKESLEAEGKTVLLLSSGDVFQGTLFFRFFRGMPD
                     IDFMNRSGYAAMALGNHEFDAGQDGMAEAFAQARFPVLSANLSFTHPRLSQQIKPSVI
                     VPVTTPHGLVKIGLIGLTTEKLHEDCPVSLLQGVQVKSAREAAQQEICRLKTEGADLI
                     FLLTHLGWNRDVELLEALPDV"
BASE COUNT          901 a         1298 c         1168 g          943 t
ORIGIN      
        1 ccgcacgaac aagccaaagg cggagtccgc cccaacctca tcgcgcgcta tcgcggcaag
       61 tctcagaaga aaacgatctg gatcatgagt cacctcgata tcgtcccccc gggcgatctc
      121 tccaagtggg actcggatcc gtacaagttg cgaattgatg gagaccgctt gtatgggcgt
      181 ggaacagaag acaatcatca gggtctggtc gccagcttgc tctgtgtcaa ggccatgatg
      241 gactgcggtc atcgccccgc ctatgatatc ggcctgctct ttgccgccga tgaggaaacg
      301 ggcagtgcct acggcgccgg ctatctggtg gaaaaacacc ccaccctgtt cggcaaggac
      361 gatatgttca tcgttcccga tggcggttac ccggatgcgt cgctggtcga ggtggccgag
      421 aagtcgatct ggtggctgaa aattcgcgcc acgggcaagc aatgccatgc ctcaatgccg
      481 caccagggca agaatgcgtt ccgagcggcc agcgccctgg tcgtgaaact gaacagtctg
      541 taccagacct tcgccagaga gaatccgttg tttgatccgc ccatcagcac attcgaaccg
      601 accaaaaaag aagcgaacgt tccgaacatc aacacgattc ctggggacga tgtgttctac
      661 ctcgacagcc gcgttctgcc ggagtacccg ttgtctgagg ttgacaagga gatcagaagg
      721 ctcgcagcgg aagttgaaaa ggaatacggg gtgtcgttcg ccttcgaaac gattcagcag
      781 ggcaacgccg cgccaccgac cagccccgac gccgaaatcg tcaccggaat cattggtggt
      841 attaaaaaag tctggggtat cacgccctct cccaaaggca tcgggggtgg aaccgttgcc
      901 gcgtttttcc gcactctgca gcttccggcc gtcgtgtatt cgaaacagga cgaagtcgcc
      961 catcaaccga atgaatactg cattctcagc aatctgatcg gcgatgccca ggtctttgcc
     1021 cacacgattc tgcatctgaa gtgaaacgtc aggcagaggc atacagccga ttcgtgcccc
     1081 gggaacgccg atcgacgtga aaatcaactg tcattgtcac attttcagtc tcgattgcgt
     1141 gccgctcggc tttcgacgag agttcgccct tgatctgcag cacccgggac atcgactgct
     1201 gcattcgact cttcacaagc tgctgaaaga tcagttcaaa ctggacacct ggctcaaatt
     1261 cgccgagttc tcgatcgggg aaatcgctga ccgactcatc gatgaaatgg acgaagccgg
     1321 tattgaggcc gccactccgc tttcggtcga tctcgaattc tgcgagaaat acggcggcac
     1381 cacgaagtct tttctacagc agatcgacga gactctggcc gcggttcagg caaccacgga
     1441 acgatgcggt cgccagcgac tgtttccgtt tatcggagcg gatccgcgcc gggaagggat
     1501 cgccgatctg atattggcgc atctgcaggc ccgccgattc ttcggtgtca agatttaccc
     1561 ggtcatgggg ttcctgccag acgacgaacg actgcagccg gtgtatgact actgcgaacg
     1621 ccagcgcgtt cccgtgacgg cccactgtca gaacggtggt ctgccggggt ttacgggata
     1681 ctacgatacc gcacacccaa acaattggcg gcccgtgctc gagcgttttc cgaagctggt
     1741 actcaatctg gctcacaatg acaccacggg cagtacgtgg caacccccgt tgtttcagtt
     1801 gatgcgcgat tttccgcacg tctacaccga tgtctcgttc gacactgaaa tgttctatca
     1861 accgggcacc tactttcgct cgatcaagtc tcttctcaac gacaagcagc tctgcgaaag
     1921 agttctctat ggcaccgact ggtacatggg gcgatttctc tggacagaga aaacctatct
     1981 tgactggttc acccgctttt cgcgaaaaat cttctgggat tccgtacaat tcaccacggc
     2041 cgaaattcgt cgtctcgtcg aagaaaaccc ggcccgattc ctcggttgga gttgaagcat
     2101 gactgattca tcgactcaca tccccgatcc cggcaacatt ccaccggcgg aacccgcttt
     2161 gaacgagaca gccctatcac cggccggtgc cactttttcc acgctctgga aaaaacttca
     2221 ggcgatcggg actctgcccg aagattctcc ggacgagcgc acgcgcaaaa ccgtgttgac
     2281 cctgatggcc tttctcagca tcgcgatcgg cctgctgctg ggcctcttcg acgtactgac
     2341 gggtttctat tatggcgccg tcattcccac tctgtatgcg gcagtttccg ccattggact
     2401 ggtacatttt tctcgcaccc ggaatctgcg attcattcgg cttctcgttc ttggcgggtt
     2461 actgatctgc cccttctgcg ccatgtggct tcggggcggg ttcgccaact cgggcgcatc
     2521 gatgatctgg gctctgggtg cgccggtgtt cggcgtcatg tttcacggtc aggcgcgttc
     2581 gattcgctgg tttgtcgcct atcttgccct gttgctgctc tcggtcctga tcgacccggc
     2641 ctgcgcccgc ttcgcgatcg agcatcagat agccccatcg agctggtatt ttctcgcaca
     2701 cattggtggc atctcgttct gtctgttcgt gctcattcaa ttcttcgttc agcggctcgg
     2761 cgaggaacag gcaaagtcag aatccctgct gctgaacatc cttccccggc aggtagccgg
     2821 ccgactcaag cacagctcag ccacgatcgc tgatggcttt caggaagcga ccattctctt
     2881 cgctgacctg gcgggattca cccgactctc ttcgtcactg gctcctggtc aggtcgttca
     2941 cctgctcaac gaagtttttt ccgcgttcga tcgtctggcc gagaaacacg ggctcgaaaa
     3001 agtgaaaacg atcggtgacg cctatatggt cgtcggcggc attccggaac ccgcgcccga
     3061 tcatccacac gccgttgccc gcatggcact cgagatgcgt gaaacggtcg ctgaactcaa
     3121 tcgacaacac aactgggaac tgcgcctccg cattggtatc aatactggcc ccgtcgtcgc
     3181 aggcgtgatt gggctgaaga agtttatcta cgacctctgg ggcgatgccg tgaacatcgc
     3241 cagccgcatg gagtcaaccg gtatcgagaa cgagattcag gtcacgcaaa gcacctacga
     3301 acgcctgcac cccgcgttta cgctcgaacc gcgtggaaaa gtccaggtca agggcaaagg
     3361 cgaaatggag acctggctcc tcaagagcgt cgccgccgtg ggccagacct gactcccctt
     3421 cccactcctg caacccccgt ccgtcgacca cggataagac gcgaactgct ggctgaggca
     3481 gaggcgatcg agctttccgg ctggtccgcg gggtttcgtg aaatccctgg tgagagcaac
     3541 ccgtgaagtg tggtatgctg aaaaaaacga ggttttcaag gaaaggacat cgcatggccg
     3601 agatcgcgac acgacggctt ttcatcgccc tctttctttt cgccgtatgg tttggcgtat
     3661 ccgtcgcttc ggcggcgaca cccgagaggg gcgtgtccct gaccattctt cataccagcg
     3721 acattcacgc ccgcctgctt ccctatgact gcgcctcagg caccgccatg ggcgggtatg
     3781 cccgaatcaa agcctacaaa gaatctctcg aagccgaggg aaaaacagtg ctgttgctct
     3841 cttcaggaga cgtctttcag ggcaccctgt tctttcgctt ctttcgcggc atgcccgata
     3901 tcgatttcat gaatcgctcg ggatatgcgg ccatggctct tggcaatcac gagttcgacg
     3961 ccggtcagga cggcatggcg gaagcattcg ctcaggccag gtttcccgtg ctgtcggcca
     4021 acctcagctt cacgcatccc aggttgtccc agcaaatcaa gccttccgtc atcgtaccgg
     4081 tgaccactcc gcacggcctc gtaaagatcg gccttatcgg cttgacaacc gaaaaactgc
     4141 atgaagactg ccccgtgtcg ctgctgcagg gagtacaagt gaagagtgct cgtgaagccg
     4201 cccagcaaga gatttgccgg cttaaaaccg aaggggccga cctgattttc ctgctgacgc
     4261 atctgggttg gaatcgcgac gtcgagctgc tcgaagccct tccggatgtc
//
LOCUS       DAORHD010000254         4301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21970_length_4301_cov_3.661328, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000254 DAORHD010000000
VERSION     DAORHD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21970_length_4301_cov_3.661328"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..>4301)
                     /locus_tag="PKO06_06675"
     CDS             complement(64..>4301)
                     /locus_tag="PKO06_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69365.1"
                     /translation="QISQDVTSSYPGQFQYTFGTTNYDNDFWDYSLGWPYTHAKYLKG
                     PFTDASGKNYMYKFSPYAPTSHQNIPLWNGPGAGAAGILHPSDPYKVVLPNKWGGISW
                     FAFGSPNAPAAHVGKLMDGTGGIPTITNYDFRLAVHGVCSPYGDAQYISAVVKREYEK
                     RDRSLDLYSYLDEVVPPPGPGPSCDAAAVLTATDLKITERYGKTCADGCIPGGDLPAQ
                     ATGKIESVVQVFTSTTGRRYGFNPYGKKTGPYGANDAALRVVHLGSTYNLDLNTTNIR
                     NAPYITTNLGTTLNAVSRLGVSSNFTTPAVPVTSSPDYLYGSVADKFCIQDSWWGVGG
                     LAFEYFIQNVTTPSGKNFLAKHIYRLDYTNSTNPTPDDVGIFDGDIDGMSVDGEGNLY
                     LLTTELDVPNSPNWPDPVGLPVGPVFAPTWPNAAITSHVDYMSNNGWWRPGVGVPDYA
                     IPSPSAAGDYLKVFFRQKVKKVCRKYTPSAGGGFSAGSVEDRGYVDGGTDTIARTLRY
                     DGPGVYSWLNDWYHPAGIGSRTASLDAEFAVVNIAARPQAYNLSPTYSICRYNRVSPS
                     AEELIEDTAYDFKLEGYKPFGANGLQQNFVYVGNLPNLGGGTYVNLIPPYENRDEDNN
                     TVAGGMPSSAFETPSYQTTVLWNVELIDPTGAVVKRYQTDAAPNHPGADECNKYKVFR
                     YAFPQPGRYRVWAKITYHTLNYSALAPGQRPSDFTGAHVVTYSNIETQKVTYTVKSNP
                     NTAVGTNEISNIVMYPTSTMVVNQSVDAIAADNGRFNIREGASPASLSFEFDVQMARD
                     ANRDLGSSFSTFGGFGKWDYGEAGGHVYNYTAGSPDNNKFHPGYPLTANPAPANRGTR
                     VDIDPTSMSAPALPSPSPSQYDLFCKDIGFITYQLYLTPPVAAMKLPANAYEIPIGTP
                     GTCTVQLVPTNVGTRKYRVRVYIPSAQLASTLRIATPIDPNTYNVRLRLNWPRVKWYE
                     HPASDAMKTYRSLQPDVDGTTSPGAPSFALNDYWIMRARDMTVLRPELDSSVVATITQ
                     TTGDPVPNAPIRFDVKDNNPCAVFSGFQVRYDVPTKQRDNAPATFNLNNAVPVKIFES
                     DKPTNPNFYASAFEYKHTYDLTNVNEYGGGTSMFAPGSSYQNWIGTLSYGIEGDIQDG
                     QGATAPLNITHQFRRNSADPFYRTDYRALVRLDNDPPSFFVQIISPSDNRRWDITLQE
                     QVADTVCNPTTVGQLANTPIHIAMFQLDTGAVLTATQTLTTIPGSSNYPTDLSGSKDV
                     DLSSIALPTGFLDALPRVRRSGRLLVNVDYADNVDYQPFQAVSVNIMERPAAGPERDL
                     LTGAKPPLPTDADYDNNGTLTSVGNQAFRARYAVDMPMTVRPAQTAPANFQVYVRIGA
                     TDSSGSQRSLLIPIQVVDSSFDARVLESKENRR"
BASE COUNT          946 a         1221 c         1208 g          926 t
ORIGIN      
        1 gaaaaccggc ccgtagaaac gggccggctt ctgccatgag tagctgtttt caggaccgaa
       61 cacttaccgt cgattctcct tgctttcgag cacgcgggcg tcgaacgacg agtcgaccac
      121 ctgaatcgga atcagcagcg aacgctgact cccgctcgaa tcggtcgctc cgattctcac
      181 atagacctgg aagttcgcag gcgccgtctg cgcgggccga accgtcatcg gcatgtcgac
      241 cgcataacgg gcgcgaaacg cctgattgcc aacgctcgtc agcgtgccgt tattgtcata
      301 gtcagcgtcg gtcggcagcg gcggcttcgc accggtcaac aagtcacgtt caggaccagc
      361 agccggacgc tccatgatgt tcaccgacac ggcctggaac ggctgatagt cgacgttgtc
      421 cgcgtagtcc acattcacca gcaatcgacc cgatcggcga accctcggca atgcatcgag
      481 gaaaccggtc ggcagagcga tcgaactcag atcgacatcc ttgctccccg atagatcggt
      541 cggatagttc gagctgccgg ggatggtcgt caacgtctgc gtcgctgtca gcaccgcccc
      601 cgtatcgagc tgaaacatcg cgatatggat cggggtgttg gccagttgcc cgacggtggt
      661 ggggttgcag actgtatcag cgacctgctc ctgcaaggtg atatcccatc gccggttgtc
      721 agacggagaa atgatctgaa cgaagaagct cggaggatcg ttgtcaagcc gaaccaacgc
      781 acgatagtcc gtgcgataga acggatctgc cgaattgcga cggaactgat gcgtaatgtt
      841 cagcggtgcg gtcgctccct ggccgtcctg aatgtcgcct tcaatgccgt aggacagagt
      901 gccgatccag ttctggtagc tagaaccggg cgcgaacatc gacgtgccac caccatactc
      961 gttgacgttc gtcagatcgt aggtgtgctt gtattcaaac gctgacgcgt agaagttcgg
     1021 attcgtcggc ttgtcgcttt cgaagatctt caccggaacc gcgttgttca ggttgaacgt
     1081 cgccggtgcg ttgtcgcgct gtttcgtcgg cacatcgtat ctcacctgga acccgctgaa
     1141 caccgcacac ggattgttgt ccttcacatc gaagcgaatc ggcgcattcg gcaccggatc
     1201 gcccgtcgtc tgcgtgatcg tcgccacgac gctcgaatca agttcaggac gcaggaccgt
     1261 catgtcgcgg gcgcgcatga tccagtaatc gttgagagcg aacgacggcg ctcctggtga
     1321 ggtggtgcca tcgacatccg gctgcagact gcgatacgtc ttcatcgcgt cagaggcagg
     1381 gtgttcgtac catttcactc gaggccagtt cagccgcaga cgcacgttat acgtgttcgg
     1441 atcaatgggc gtggcgatgc gcaaagtgct ggccaactgc gcggacggga tatacacccg
     1501 cacccgatac ttgcgcgtac caacgttcgt cggcactaac tgcaccgtac aggttccggg
     1561 ggtgccaatc gggatctcat acgcattcgc cggcagcttc atggccgcca cgggaggcgt
     1621 cagatacaac tggtacgtga tgaacccgat atccttgcag aacaggtcat actgtgaagg
     1681 cgaagggctc ggcaacgccg gagcactcat gcttgtcgga tcgatatcca cgcgagtgcc
     1741 gcgatttgcc ggggccgggt tcgccgtcag agggtaaccg gggtggaact tgttgttgtc
     1801 cggagagcct gcggtgtagt tgtacacgtg accacccgct tcaccgtagt cccacttacc
     1861 gaatccgccg aaggtcgaga acgacgaacc aagatcacgg ttcgcatcac gagccatttg
     1921 tacatcaaat tcaaagctca aagacgccgg tgaagcacct tcgcgaatat tgaaccggcc
     1981 attgtcggcg gcgattgcat caacgctttg attcacgacc atcgtactgg tcgggtacat
     2041 gacaatgttc gagatctcgt tcgtgccgac cgcggtgttc gggttcgact tgaccgtata
     2101 ggtcaccttc tgggtctcga tgttcgaata ggtcacgaca tgagcaccgg taaagtcact
     2161 cgggcgctgt ccgggagcca atgcgctgta attcagcgtg tgataggtaa tcttcgccca
     2221 cacccgataa cggccaggtt gcgggaacgc ataccggaac accttgtact tgttgcattc
     2281 atccgcaccc ggatgattcg gggccgcatc cgtctgatac cgcttcacca cagctccggt
     2341 cgggtcgatc aactcgacat tccagagaac cgttgtctga taggaaggag tttcaaacgc
     2401 gcttgatggc ataccaccgg caaccgtgtt gttgtcttcg tcacgattct catagggcgg
     2461 aatcaggttt acatacgtgc caccgcccag gttcggcagg ttgccgacat agacgaagtt
     2521 ctgctgcagg ccgttcgcgc cgaagggctt atacccttcc agcttgaagt cgtaggcagt
     2581 atcttcgatc agttcttcag ctgacgggct cacccggtta tatctacaga tcgaatacgt
     2641 tggcgacagg ttatacgcct gcggacgagc cgcgatgttc accaccgcga attccgcatc
     2701 gagtgacgcc gtacggctgc cgatacccgc cggatggtac cagtcgttca gccacgaata
     2761 cacgccggga ccatcatagc gcagagttcg tgcaattgtg tcggttccac cgtcgacata
     2821 gccgcggtct tcaaccgatc cggcactgaa accaccaccc gcgctcgggg tgtatttccg
     2881 acagaccttt ttgaccttct ggcgaaagaa taccttcaga taatcgcctg cggccgatgg
     2941 cgagggaatc gcataatcag gcacgccgac tcccggacgc caccaaccgt tgttgctcat
     3001 gtagtcaacg tgtgaagtga tcgccgcatt cggccacgtc ggtgcaaaca cggggccaac
     3061 tggcaaaccg accggatccg gccaattcgg cgagttggga acatccagtt cggttgtcag
     3121 aaggtacaag ttgccttcac cgtcgacgct catgccatcg atatctccat caaaaatacc
     3181 gacgtcatcg ggtgttggat tcgtggagtt ggtgtaatcg agacgataga tgtgtttggc
     3241 caagaaattc ttgccgctcg gtgtggtcac attttgaatg aagtattcaa atgccagacc
     3301 gccaactccc caccaggaat cctggatgca gaacttatcg gctactgatc catagagata
     3361 gtcgggagaa ctggtgaccg gaaccgcggg agtcgtaaag ttcgacgaaa caccaagacg
     3421 actgaccgca ttcaacgtcg taccaaggtt cgtcgtgata tacggagcgt tgcgaatatt
     3481 cgtggtattc agatcgagat tataagtgct ccccagatgg acaactctga gagccgcatc
     3541 attggcgcca tagggaccgg tcttttttcc atacggattg aatccgtatc ggcgaccggt
     3601 ggtcgaagtg aagacctgaa cgacggactc gatcttgccc gtcgcctgag cgggcaagtc
     3661 ccctcccgga atacatccgt cagcgcaggt cttgccatag cgttcggtga ttttcaagtc
     3721 cgtggccgtc aacaccgcag ctgcgtcaca actgggcccg ggacccggag gagggacgac
     3781 ttcatcaaga taactgtaca agtcgagaga acgatctcgc ttttcgtatt cgcgtttgac
     3841 aaccgcagaa atgtattggg catccccata cgggctgcac acgccgtgaa cagccaatct
     3901 gaaatcgtaa ttggtgatgg tcggaatgcc tcccgtccca tccatcaact tgcccacgtg
     3961 cgctgcagga gcgttcggtg aaccgaaggc aaaccacgag atccctcccc acttgttggg
     4021 caacacgact ttgtacgggt cactcggatg caaaataccc gctgcaccag cgcccgggcc
     4081 attccacaac ggaatgttct gatgtgacgt aggcgcatac ggactgaact tgtacatgta
     4141 gttttttccg ctggcatccg taaatgggcc tttgagatac ttcgcatgcg tatagggcca
     4201 accgagggag tagtcccaga aatcgttgtc gtagtttgtc gttccaaacg tatactggaa
     4261 ctggccagga taagaagagg tgacatcctg ggaaatctgc g
//
LOCUS       DAORHD010000255         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_21990_length_4299_cov_4.050189, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000255 DAORHD010000000
VERSION     DAORHD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21990_length_4299_cov_4.050189"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PKO06_06680"
     CDS             complement(<1..383)
                     /locus_tag="PKO06_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV69366.1"
                     /translation="MNTRHYFVSIIVIFQLICALSASAEAAMVVTSAKEENSSDAPIA
                     LREPPAMPMAAGSAESETSGTETEGGSVFYNRQLIQATLGKTRQVLLTFDDGPHPNAT
                     PLILDTLKKHGLKAVFFVVGMNVRK"
     gene            670..3327
                     /gene="clpB"
                     /locus_tag="PKO06_06685"
     CDS             670..3327
                     /gene="clpB"
                     /locus_tag="PKO06_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNV69367.1"
                     /translation="MDANRMTQKSQEALQQAQNIAVRYGHSEVDAEHLLSALLEDQQG
                     LISRLFNRMDVPLETLKQRLDAELSRRPRVSGPGMQPGQIYISRTFSQLLVVAEEEMK
                     RFRDEYLSVEHLVLAMVASGDQTAAGRLLASLKIDRDRLLQALTEVRGNQRVVSANPE
                     QTYEALEKYGRDLVREAQRGKLDPVIGRDDEIRRVIRILSRKTKNNPVLIGEPGVGKT
                     AIVEGLAQRILRRDVPDGLKDKTIFALDMGALIAGAKFRGEFEERLKAVLAEIKQSEG
                     RIILFIDELHTIVGAGKAEGSMDAGNMLKPMLARGELHCIGATTLDEYRKHIEKDAAL
                     ERRFQPVLVEQPDVADTISILRGLKERFEVHHGVHIHDQAIVVAATLSQRYISDRFLP
                     DKAIDLIDEACAMIRTDIDSMPADLDAVQRRIMQLQIEEAALAKEKDEASRTRLEELR
                     RELAEQKNRADGMKAQWESEREVIKKAQLCREEIEQLHRKIEEAERVYDLNLAAELKH
                     GRLPQLQSQLKAVEEELKRKQSTGRLVREEVTDEEIAEIISRWTGIPITKLVEGEREK
                     LLRLDAILHERVVGQDEAVQVVADAVIRSRAGIKDPRRPIGSFVFLGPTGVGKTELAK
                     ALAQSLFDTEENLVRIDMSEYMEKFAVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVV
                     LFDEIEKAHPDVFHVMLQILDDGRLTDSQGRTVDFKNCVLIMTSNIGSQHLLEGLDKD
                     GMVTDQVRERVLGEMRHSFKPEFLNRIDDIVLFKPLGMSELGKIIDLLAADLRKRLIE
                     RRMTLTFSDEARTWFIQHGYSPTYGARPLKRLLQKELETRLGRALISGELRDGSSILV
                     ELRQGEPTLRIEADLRNGNGKGNGERAHA"
     gene            3479..4024
                     /locus_tag="PKO06_06690"
     CDS             3479..4024
                     /locus_tag="PKO06_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69368.1"
                     /translation="MFLRTFLLRWVISIVAAFVLLLVIDPQYFDRPLAEGTAGLAAAE
                     APSGPFATLIGVDIPDGSQLVSVFLKPRDVSLEARGEFLDPQGEVIMAESFVLASDTA
                     GQKARWTIWKVAPRGAGKYAIRLTQTVPGRITLYFYQGPFWERMLGLPLVLALINLIA
                     LFARRSRIEPQSASITPPSDV"
BASE COUNT          963 a         1090 c         1306 g          940 t
ORIGIN      
        1 tactttcgca cgttcatgcc gacgacaaaa aagactgctt tcagaccatg cttcttcaag
       61 gtgtcgagaa tgagcggtgt tgcattcgga tgcgggccgt catcgaatgt cagcagaacc
      121 tgacgtgttt ttccgagcgt ggcttgaatc agttgacggt tgtaaaagac gctgccacct
      181 tctgtttcag tcccgcttgt ctcgctttct gcgcttccgg ccgccatggg catggctggc
      241 ggttcgcgta aagcgatggg cgcatcgctc gagttctcct ccttggcgga ggtgacgacc
      301 atcgcagctt cggcagacgc cgagagcgca caaatcaact gaaagatcac gatgatggag
      361 acaaagtaat gacgagtgtt catgttcggt gtatcggcat tctatgatct cagattgagg
      421 tcattttgcc aagagggcgg cgacggtttc gctcgaacga gcagcgtcgc agtgacgtcg
      481 cagtggggga gatttccgtt cggtctgcga gggacggcgt tgacgaaaac agggcatgat
      541 cacgttttct cagctttgcg acctgcgatg ggacgggaat tcgctcttcg gaattgtctg
      601 gtttcgagac tggcatgaat tttgtattgt gaatggctac aaccttcgtt gaagacagga
      661 gaaacactca tggatgccaa ccgcatgact cagaaaagcc aggaggctct gcagcaggcc
      721 cagaacatcg cggtcaggta cgggcattcg gaagtcgatg ccgaacacct gctgtcggcg
      781 cttctcgaag accagcaggg gctgatttcg cgactgttca accgcatgga tgtgccgctg
      841 gaaacactga aacagaggct tgatgctgag ttgtcgcgtc gtccacgcgt gagcggacca
      901 gggatgcagc ccgggcagat ctatatttct cgcacgttct cgcagcttct cgttgttgcc
      961 gaagaggaaa tgaagaggtt cagagacgag tatctgtccg tggagcatct cgtgctggcc
     1021 atggtcgcgt cgggtgacca gaccgcggct ggccgtctgc tggcctcgct gaagatcgat
     1081 cgtgatcgtc tgttacaggc actgaccgaa gttcgcggca atcagcgggt cgtgtcagcg
     1141 aaccctgagc agacctacga agcactggaa aagtacggtc gcgacctggt gcgtgaagcg
     1201 cagcgcggca agctcgatcc ggtgattggc cgcgatgacg agattcgtcg ggtgatacgc
     1261 attctgtcgc gcaaaacaaa aaataacccc gttctcatcg gtgaaccggg ggtcggcaaa
     1321 acagcgatcg ttgagggatt ggcacagcgc attctgcgtc gtgacgtgcc agacggtctc
     1381 aaggacaaaa ccatctttgc gctcgacatg ggcgcactca ttgcaggagc caagttccgc
     1441 ggtgagttcg aggaacgttt gaaagccgtg ctggccgaga tcaaacagag tgagggtcga
     1501 atcattctct tcattgatga gctgcatacg atcgtcgggg ccgggaaagc ggaaggttcg
     1561 atggatgccg ggaacatgct caagcccatg ttagcccgcg gcgaactgca ttgtatcggt
     1621 gcgacgactt tggatgagta ccgcaaacat atcgaaaaag acgcggcact ggaacggcga
     1681 tttcagccgg tactggttga acaacccgat gttgctgata caatctcgat attgcgtggg
     1741 ttgaaagagc gtttcgaagt tcatcacgga gttcatatcc atgatcaggc aattgtcgtg
     1801 gcggccacct tgtcgcaacg ttacatctcc gatcggtttc ttcccgacaa agccatcgac
     1861 ctcatcgacg aggcatgcgc catgatccgc accgacattg attcgatgcc ggcggacctc
     1921 gatgccgtgc agcgtcgcat catgcagctg cagatcgaag aagccgccct ggccaaagaa
     1981 aaagatgaag cgagccggac tcgcctggaa gaattgcgtc gggaacttgc cgagcagaaa
     2041 aacagggctg acggcatgaa agcgcagtgg gaaagtgaac gcgaggtgat caagaaagcg
     2101 caactgtgcc gtgaagagat cgagcagctg catcgaaaaa tcgaagaagc tgaacgggtc
     2161 tacgatctga atctggcggc cgaactgaaa catggtcgtt tgcctcagct gcagagccag
     2221 ctcaaggccg tagaagaaga gttgaagcgc aaacaatcga cggggcggct ggttcgagaa
     2281 gaagtaaccg acgaggagat cgccgagatc atttcccgct ggaccgggat tccgatcacg
     2341 aagctcgtcg agggcgagcg cgaaaaactg ctgcgactgg atgcgatttt gcacgaacgg
     2401 gttgtcggtc aggatgaagc cgtgcaggtc gtggccgacg cggttatccg gtccagagcc
     2461 ggaatcaagg accctcgccg cccgatcggt tcgtttgtgt ttcttggccc gaccggcgtt
     2521 ggcaagacgg aattggcgaa agcccttgcg caatcgctct ttgacaccga ggagaacctg
     2581 gtccgtatcg acatgagcga atacatggaa aaatttgccg tgtcgcggtt gattggcgcg
     2641 ccgccaggat atgtcggtta cgacgaggga gggcagctga ccgaggcggt gcggcgtaaa
     2701 ccgtattccg tggtgttgtt cgatgagatc gaaaaagcgc acccagacgt gtttcacgtg
     2761 atgctgcaga ttctcgacga tggtcgactg actgattccc aggggcgaac cgtcgatttc
     2821 aagaactgcg tgttgatcat gaccagcaac attggttcgc aacatctgct tgagggtctc
     2881 gacaaggatg gaatggtcac cgatcaggta cgcgaacggg tgctcggaga aatgcggcat
     2941 tcgttcaaac ctgagttttt aaaccgcatc gatgacatcg tgttattcaa accactcggc
     3001 atgtcggaac tcggcaagat catagatctg ctggcagccg acctgcgaaa acgtctcata
     3061 gagcggcgca tgaccctgac cttttcggac gaagcccgca cgtggttcat tcagcacgga
     3121 tacagtccga cgtatggagc ccgtccgctg aaacgtctgt tgcagaaaga gctcgagacc
     3181 cgcctgggcc gcgcgttgat cagtggtgag ctgcgcgacg gatcgagcat tctcgtcgaa
     3241 cttcgtcagg gtgaacccac gctgcgcatc gaagcagacc tgcgcaacgg caacggaaag
     3301 ggaaacggag agcgcgctca cgcatagatg ggaaagactg cgtctgagca gttcaatcgc
     3361 cgattggtag cagcagtcgc gaccttccct gcggtgggcg tgttctagat ctgcttgcca
     3421 ggctgctctt ggggtgtgtt atgctgtcgg tcatcctgcc gtgcgagagg tgaccgtcat
     3481 gtttctcaga acgtttctgc tgcgatgggt gatctccatc gttgccgcgt ttgtgctgct
     3541 gttggtcatc gatcctcagt atttcgatcg tccactggcg gaaggaacgg cagggcttgc
     3601 ggcggcagag gctccatcag ggccatttgc cacgctgatc ggggttgata tacccgatgg
     3661 aagccagctg gtttctgtat ttctcaagcc acgcgatgtc tcgctcgaag ctcggggaga
     3721 gtttctcgac cctcagggcg aggtcattat ggcagagtct ttcgtgcttg ccagtgatac
     3781 agcaggtcag aaagcacgct ggaccatctg gaaagtggca ccgcgtggtg cgggcaaata
     3841 cgccattcgg ctgacccaga cggttcccgg tcgaattacg ttgtattttt atcagggacc
     3901 tttctgggaa cgcatgctgg gcttgcccct ggtattggcg ctgatcaatc tgatcgcctt
     3961 gtttgccaga agaagtcgca ttgagccgca gagcgcgtcg ataaccccgc cctctgacgt
     4021 gtaactttag gctctgcgag tctgagaaag ggcgcgaggt catccacagg gtgacacaga
     4081 cacggacgga attgtctgaa ccgttcaaag acgggttgta cgagggtgtc attctgaagg
     4141 gtgtgtcgtg actgacgctc atcccatcga tgtttccggt tgcctgcctc ccgatgatcc
     4201 ctccgtgtca gagattctca tgacaggcct ttacgctttt cggtttgatc attagaattg
     4261 tacaaactat cgatcgggag gctgcaggta tgaaaaatc
//
LOCUS       DAORHD010000256         4290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22066_length_4290_cov_3.631641, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000256 DAORHD010000000
VERSION     DAORHD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22066_length_4290_cov_3.631641"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..803)
                     /locus_tag="PKO06_06695"
     CDS             complement(63..803)
                     /locus_tag="PKO06_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69369.1"
                     /translation="MFSSSLHCRSWGVNRGEEEFLGPVGMITLAWCSATCPHCYLSEQ
                     QPEIVQVPLDAFPHIIAEMRERGAKSLYIVNPERQGEPLTRVLRTCRETFPDFPVVLK
                     LGGHESSAEIEPLLDHVDACSLDVKAFCADTAAALGLPDLLTRTEQLVHLVSDKLGRF
                     QQTTRGVAGWWARHVGLPGRVANLEPEIRRRLGDHRPPIWFSREYRPFGRARKMNMFN
                     RKLTSEERDSMCKLVQKLATEGWPAWLR"
     gene            complement(790..1995)
                     /locus_tag="PKO06_06700"
     CDS             complement(790..1995)
                     /locus_tag="PKO06_06700"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNV69370.1"
                     /translation="MSEYRKILSEQEVKNGQVCWDEKQDAEFRRVIPETLVFDMTLNG
                     VHLEHLTIDWERRVLTIGDQLKQAVPGSEIVLAAPAERGGVTVQGRLYQPGERVMVRK
                     RLSASECKQRAIKWYAREDDLYRRLFPAEKHFAVEIHGKLITNRAPDFERRILVIGDA
                     LRVFAPGDSLLLHKASAAPLPTLVIEKEVPRTDPSDDSKTIRAMVTRLISRPLQEFNE
                     GEVKGLIALLDENKKLWERLHQLREENEKLKEQVSTLENVFDHLTRNSFFHSKGEFED
                     WVATHIGAIEKGVRPLHRDYQVTLDGGKPRRIDLLCQDRKGVLIAVELMYNPRQEDIK
                     GTVALLAWLQGNISRLGRELTGGQLQAASIRGMVVTNRERPDLVEMCLQNGIKLCVIN
                     SGFVVDVLE"
     gene            complement(1998..2846)
                     /locus_tag="PKO06_06705"
     CDS             complement(1998..2846)
                     /locus_tag="PKO06_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69371.1"
                     /translation="MKPMANCLVIVRVVVLLCCLLKALTPVWSQSLQMTPQLLFAEME
                     RKAGGIDSLIAELELASGSLRATVQLAIQSPDKFSMDFQGRVIRVVFDGERLWIHINA
                     LNEVFLLDTSKGGSWLGDVLRDYVNPRRIITQVTRKTLFSFFDVSMLASPSIIPSFDE
                     PLAEPATAGYCLRFTPILGDWARKIFDVGYYEMVFSRTTFLPYLVVEYSTQGKLRGSL
                     RVFEYRMNEVLPKERFEFTVPEGVKVVPLAEVVMQKLEEAKDTLVDNTQKWMDGLQKQ
                     ISDWGF"
     gene            complement(2843..3715)
                     /locus_tag="PKO06_06710"
     CDS             complement(2843..3715)
                     /locus_tag="PKO06_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69372.1"
                     /translation="MELQKLAQDARLLLQSRGSTEIPAFTGMLPFAWAHPDLLIQALR
                     TGTNDRFFLQPALQGPESRGVERFSPYLVWFLQPFSAGWLTRLCELVSALLGNTDSGA
                     ASFEMSGRKPSPQMFVAPGAVWQVSWQAAPIGEIQVWTKLLGEDLTAPAGVCVVNLNQ
                     ISAIRAGEASMPQTPWSSRLPAEEILAWQRSPRLAPDAEGLFPARLAQERWEGLRTQI
                     DAHLGNRRWLEAYRTSMEAAVLLLDGQACGLSGETFRAGVEPVLQELLNRIPPLLQRK
                     STGTGKGTREAVLR"
     gene            3813..4154
                     /locus_tag="PKO06_06715"
     CDS             3813..4154
                     /locus_tag="PKO06_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV69373.1"
                     /translation="MNVWTPEAFQQDILNQHPLVLVSFSAEWCEPSRLQVPILQTLRS
                     SYADRAHIEIFDVDQQTPLADHWQVRTLPASLLFAKGELVEALAGFQAREYLQEYLDY
                     LSESLKQTPTT"
BASE COUNT          934 a         1340 c         1121 g          895 t
ORIGIN      
        1 attccgcgag ctttgccgca agcgctccgc tcgaatgact tccacccagg aaggagtctg
       61 attcatctca gccatgccgg ccacccttct gtcgcaagtt tttgtaccag cttacacata
      121 ctgtcacgtt cttcagatgt cagttttcta ttgaacatat tcattttccg cgcgcgccca
      181 aaggggcgat actcacgtga aaaccagatc ggcgggcgat ggtcacccag tcgccggcga
      241 atctccggtt cgagattggc cacccggcca ggtaatccca cgtgccgcgc ccaccacccc
      301 gccactcctc gcgtcgtttg ctgaaaccga cccagcttgt cactcaccag gtggacgagt
      361 tgttccgtcc gggtcaggag atccgggagg ccaagcgccg ccgccgtatc ggcgcaaaac
      421 gctttgacat cgagagagca ggcatcgacg tgatcgagaa gcggttcgat ctccgcgctc
      481 gactcgtgtc ccccgagctt gagtacaacg ggaaaatcgg ggaatgtctc acgacacgtt
      541 ctcagaacgc gagtcaatgg ctctccctgt cgctcggggt tcacgatgta caatgatttc
      601 gctcctctct ctctcatttc cgcgatgatg tgcggaaaag catcaagagg aacctggaca
      661 atctctggtt gttgttctga tagatagcag tgggggcacg tggcggaaca ccaggccaga
      721 gtgatcattc cgaccggtcc gaggaactcc tcttcgccac ggttcactcc ccaggagcgg
      781 caatgcaggc tactcgagaa catccaccac aaatccgctg ttgatcacac agagtttgat
      841 cccattctgc aaacacatct ccacgagatc gggccgttca cggttggtca cgaccatgcc
      901 gcgaatggac gctgcctgca actgaccgcc cgtcagttca cgccccagtc ggctgatgtt
      961 cccctgcagc caggccagaa gggccaccgt ccccttgatg tcttcctggc gagggttata
     1021 cataagctcg acggcaatca gcacaccttt acggtcctgg cagagcagat cgatccgtcg
     1081 cggttttccg ccgtcaagcg tgacctggta gtcacgatgc agcggccgga cgcctttctc
     1141 gatcgccccg atgtgcgtgg cgacccaatc ttcaaactct cctttggaat ggaaaaacga
     1201 gttgcgggtc aggtgatcaa acacgttttc cagcgtgctg acctgttcct tcagcttttc
     1261 attctcctca cgcagctggt gcaaccgctc ccaaagtttc ttgttttcgt cgagcagcgc
     1321 gatcaggcct ttaacctcgc cttcgttgaa ctcctgcaga ggtcttgaga tcaggcgggt
     1381 caccatcgcg cgaatcgttt tcgagtcatc gctgggatcg gtgcgcggaa cctctttttc
     1441 aatgaccagg gttggcaacg gcgcagccga agccttgtgc agcagcagag agtcacccgg
     1501 agcgaacacc cgcaaggcgt cgccaatgac cagaatccgc cgttcaaaat caggcgcacg
     1561 gttggtgatc agctttccat gaatctcgac cgcgaaatgt ttctcggcag gaaacaggcg
     1621 tcggtacaag tcgtcttcgc gggcatacca cttgatcgct cgttgcttgc actccgatgc
     1681 cgaaagccgt ttgcgcacca tgacccgttc gccgggctgg tacaatcgcc cttgaacggt
     1741 aaccccacca cgttcggctg gtgctgccag aacgatctct gaaccaggga cagcctgttt
     1801 cagttgatcg ccgatcgtca acactcgccg ttcccagtcg atcgtcagat gctcaagatg
     1861 cactccgttg agagtcatat cgaacaccag tgtttcagga atgacccgac ggaactccgc
     1921 atcctgcttc tcatcccagc agacctggcc gtttttgact tcctgctctg acaaaatctt
     1981 gcgatattca gacatgatta gaatccccag tcactgatct gcttctgcag cccatccatc
     2041 catttctgtg tgttgtcgac cagcgtatcc ttcgcttcct ccagcttctg catcaccacc
     2101 tcggcaagcg gcacgacttt cacgccttca ggaaccgtga actcaaaccg ctctttcggc
     2161 agaacctcgt tcatgcgata ctcgaaaacg cgtagacttc cgcgcagctt cccctgcgtc
     2221 gaatactcca cgaccagata aggcaggaat gtcgtgcgcg aaaaaaccat ttcgtaatag
     2281 cccacgtcaa agatcttgcg cgcccagtcc ccgagaatcg gggtaaatct caggcagtaa
     2341 cctgccgtcg ccggctccgc cagaggttca tcaaaactcg gaatgatgct gggtgaggcc
     2401 agcatgctca catcgaagaa cgaaaacagg gttttgcggg taacctgggt gatgattcgc
     2461 cgcgggttca cgtaatcccg caaaacatct cccagccagc tgccgccttt ggacgtgtcc
     2521 agcaggaaca cttcgttcaa ggcattgata tgaatccaca ggcgttcgcc atcaaagacc
     2581 acgcgaatca ccctgccctg aaagtccata ctgaatttgt ccgggctctg aatcgccagt
     2641 tgcaccgtgg cacgcaggga accgctggca agttcaagtt cggcaatcag cgaatcgatg
     2701 ccaccggctt tgcgttccat ttccgcgaac agcaactggg gtgtcatctg cagagattga
     2761 gaccaaaccg gtgtcagagc tttcaacagg cagcaaagaa gaacgacgac ccggacaatc
     2821 acgagacaat ttgccatcgg cttcatcgca acacggcctc cctcgtcccc ttgcctgtac
     2881 cagtcgactt gcgctgcaac aacggaggaa tgcggttaag caactcttgc aacacaggtt
     2941 cgaccccggc tcgaaacgtt tcaccggaca atccgcaagc ctgcccgtcg agcagcagca
     3001 ccgccgcttc catggaggtg cggtaggcct cgagccagcg tcggttgccg agatgggcgt
     3061 cgatctgcgt gcgcagccct tcccagcgtt cctgtgccag acgagccgga aacaacccct
     3121 cggcgtcggg tgccagacga gggctgcgtt gccaggccag aatctcttcc gccggcaggc
     3181 gggaactcca tggcgtctgc ggcatcgagg cttcacccgc gcgaatagcg gagatctggt
     3241 tcaggttcac aacgcacaca ccggccgggg ctgtcaggtc ttcgcccagg agttttgtcc
     3301 aaacctggat ctcgccgatg ggcgcagcct gccagctcac ctgccagacg gcccccgggg
     3361 caacgaacat ctgtggtgac ggctttctcc cgctcatttc aaacgacgca gcccctgaat
     3421 ccgtgttgcc caacaaggca ctcaccagct cacacaatct cgtcagccag ccggcggaga
     3481 acggctgcag aaaccagacc agataggggg aaaagcgttc gacgccacgc gactcaggcc
     3541 cctgcagcgc cggctgcaga aaaaagcggt cattcgtgcc ggttctcaga gcttgaatca
     3601 acagatcggg atgagcccag gcaaagggca gcataccggt gaatgcggga atctcggtgc
     3661 tgccgcgact ctgcaagagc agtcgagcat cctgagccag tttttgcagt tccacgcgtg
     3721 ctccttcggc cggtcatgat ggggaagatg tgctattata ccgaatgtct gtggcacaaa
     3781 caaatgttcc cgtctctctc ggaaggcaaa acatgaacgt ctggaccccg gaagcatttc
     3841 aacaggatat cctcaatcag cacccactcg tgctggtgtc attttccgcg gaatggtgcg
     3901 aaccaagtcg cctgcaggtg ccgattctcc aaacgttgcg ctcctcttac gcagatcgag
     3961 cgcacatcga gatcttcgat gtcgatcaac agacgcctct tgctgatcac tggcaggtac
     4021 gaacactccc cgccagtctg ctgttcgcca agggggaact cgtcgaggct ctggcgggtt
     4081 ttcaggcccg ggagtacctg caggaatacc tggactatct gtcggaatct ctcaagcaga
     4141 ccccgacaac ctgagtcgct ccagtcggcg acacgtgcca tcgtccgcaa tctctgccag
     4201 caccgcattg agctggcttg aaccttcggc catttcgaag cgttccgaga accctctgac
     4261 aaagcgctga atcacgatat cggagcgaac
//
LOCUS       DAORHD010000257         4287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22089_length_4287_cov_2.603733, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000257 DAORHD010000000
VERSION     DAORHD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22089_length_4287_cov_2.603733"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PKO06_06720"
     CDS             complement(<1..117)
                     /locus_tag="PKO06_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNV69374.1"
                     /translation="MSDSASVASGVPVYLIGNPNCGKTSLSIEKTGEHQHVGN"
     gene            complement(114..362)
                     /locus_tag="PKO06_06725"
     CDS             complement(114..362)
                     /locus_tag="PKO06_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HNV69375.1"
                     /translation="MVKLPLTLADVPLGKRARIRDIESGSPLGRRLLDLGFVPGTSVV
                     AIRRAPFGDPIAVMVRGSLYGLRHNEARLIQLEDSDHE"
     gene            complement(661..1728)
                     /gene="nadA"
                     /locus_tag="PKO06_06730"
     CDS             complement(661..1728)
                     /gene="nadA"
                     /locus_tag="PKO06_06730"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HNV69376.1"
                     /translation="MTTVGLLSPAENEREVIERIHAHKRRLGSDLVILGHHYQTDTVI
                     QCADFTGDSLELARHAAATTARLIVFCGVHFMAESADILTRPDQTVVLPNADAGCPMA
                     DMASVDELESAWLQLGRIMSPDEQIMPVTYINSGAAVKAFCGLRGGLTCTSANARKIL
                     TWALTKRPVVFFFPDEHLGRNTALSLGIPHDQMLVWNPAQPFGGNTPDQLRKARVLLW
                     QGHCHVHSRFRRVHVEQARLAHPQALVAVHPECRSEVAQAADATGSTGFLITYVDQAP
                     PGSTIIVGTEINLVGRLARTRLDRTVLPLGQGLCPNMFKNTAADVLEVIEQEGRKNAI
                     RVAAEIAEPARVALNRMLQAS"
     gene            complement(1725..3257)
                     /locus_tag="PKO06_06735"
     CDS             complement(1725..3257)
                     /locus_tag="PKO06_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69377.1"
                     /translation="MATSSRTTAVNAIKKALVAGFPVIALVTSEEDQAENAIHSISRM
                     AFAQEGSFQTWDLQQGLRVITPEGARPTIDGTIGLLPALDYLLSQPSERGAILFKDLA
                     FFLPQPEVQRRLKAFATQYRGQNRFLFLVGTQILLPVELRQDIHLIEFSLPDVNELQE
                     IVQRAAQNCARRGAENQLSPDEVSQAAVALQGFTASEAQFTLNKVLWGRKTINAEILP
                     QLHSEKEQLVRKEGILEYYRSQTSLEDVGGLQNLKEWLVKRKKLFSPEAEAAGLHPPR
                     GLLMMGISGCGKSLSVKAISSLWNLPLFRLDMNQVYSGMHGSPEGTFHRAIQIIEAVA
                     PAILWIDEIEGGISSSTAKDGSTGSHIFSAFLTWMQEKSNQVFVAATANRIDLLPAEI
                     IRKGRFDQVFFIDLPNDHEREDILRVHLRQRGNDPAKFDLPFLSVATEGWNGAEIEHV
                     VEAAMIEAFSRGEPMIQDDLYTIIRSTVPLARTMSEQIKFIKGWASERAISASHSGKS
                     IA"
     gene            complement(3261..3461)
                     /locus_tag="PKO06_06740"
     CDS             complement(3261..3461)
                     /locus_tag="PKO06_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69378.1"
                     /translation="MKTRHDTEMAPRSYSLYDPVTGFAYTYDLFEKDLYRQSVTQSNA
                     SGNQPTAGSHRLSASSDRLTLP"
     gene            3644..>4287
                     /locus_tag="PKO06_06745"
     CDS             3644..>4287
                     /locus_tag="PKO06_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69379.1"
                     /translation="MKQYLSLFFVMCGAFACLIGPVYASEPSMLQQLDAAQIQEFQGR
                     AHSVTKQLALVNTIERLLDCAASGRINVSLIGLDGVPTWVREMWQRHEPDAVEVSVSC
                     YAMALDAKQPAARTDFYRFRSTRTNGEIVESLLQDIRKDKKAAVIFTFIDGDPFGHDK
                     EIQRVLGELLAAIEEGEVVVKNLKLADAPLRVNNLWRANAIPGYIPERVLVQQYL"
BASE COUNT          903 a         1142 c         1325 g          917 t
ORIGIN      
        1 gttcccgaca tgctggtgct caccagtctt ttcaatgcta agactggtct tgccgcagtt
       61 cgggttaccg atgaggtaga ccggaacccc agaggcgact gaagcgctgt cgctcattcg
      121 tggtcagagt cttcaagctg aatcagacgg gcttcgttgt gccgcaaccc gtacagagaa
      181 cctctgacca ttaccgcgat cggatcgcca aacggagccc ggcgaatggc cacaaccgaa
      241 gtgccgggaa cgaacccgag gtcgagaagg cggcggccga gcggtgagcc gctctcgata
      301 tcgcgaatgc gcgcacgttt ccccagtggt acgtctgcga gtgtcagtgg aagttttacc
      361 atggtgtgta ccgtctgccg caagttagac taatctaata acagaatact cttctaacaa
      421 tgaaaggtca agctttcttt tgtatggtct cgagaagtcg tattgaaccg gtttgggcaa
      481 tacggtggct aaacatcaga atctacagtg agccggtcag cgatttgcca gggagtgact
      541 gtgtgacagg aagggggttg actggtctac cttcaagatt gtgctactga actgtagggc
      601 agcacctgaa ggactgtggt cgagaaaggg gagtcagggc cggcagggcg gggattcgtg
      661 tcagctcgcc tgcagcatgc ggttcagagc gactcgggca ggctcagcga tctccgcggc
      721 cactcgaatc gcattttttc ggccttcctg ttcgatcacc tcgaggacgt ctgcggcggt
      781 gtttttgaac atgtttgggc agaggccctg tccgagcggc agcaccgtgc ggtcgagtcg
      841 ggtgcgcgcc agacgaccca ccaggttgat ctcggtaccg acaatgatcg tgcttcctgg
      901 tggagcctga tcaacgtagg tgatcaggaa tcccgttgaa cccgtggcat cggccgcctg
      961 tgcaacttca gatcggcatt cgggatgcac ggcaaccaga gcctgcgggt gtgccagacg
     1021 cgcctgttcc acgtgcacgc gacgaaaccg ggaatgcacg tgacagtgcc cctgccagag
     1081 caggacgcgt gccttgcgca gttggtcggg cgtgttgccg ccaaaaggtt gcgctgggtt
     1141 ccagaccagc atttgatcgt ggggaatgcc gagggacaga gcggtattgc gccccaaatg
     1201 ctcatctggg aaaaagaaca cgaccggtcg cttcgtgaga gcccaggtga gaatcttgcg
     1261 ggcattggca gaggtgcagg tcagtccgcc ccgcagaccg cagaaggctt tcacggcagc
     1321 gccagagttg atgtaggtga ccggcatgat ctgttcgtcc ggggacatga tgcgcccaag
     1381 ctgcagccag gctgattcga gttcatcgac gctggccatg tcggccatcg gacacccggc
     1441 atccgcattg ggcaacacga cggtttgatc ggggcgtgtc agaatgtcgg cagactcggc
     1501 catgaaatgc accccgcaga agacgatcag gcgcgctgtc gtggcagccg catggcgggc
     1561 gagttccagg gagtcgccgg tgaaatcagc gcactgaatg acagtgtcgg tctggtaatg
     1621 atggccgaga atgaccaggt cactgccaag acgccgcttg tgagcgtgaa tacgctcgat
     1681 tacttcccgt tcgttctcag caggtgacag aagtcccacc gtggtcatgc gatgcttttt
     1741 ccggaatggg atgcggaaat ggcgcgctcg cttgcccagc ctttgatgaa cttgatctgc
     1801 tccgacatgg tgcgggcaag aggcacggtt gagcgaatga tggtgtacag gtcgtcctga
     1861 atcatcggtt cgccacgaga gaaggcttcg atcatggccg cttcgaccac gtgctcgatt
     1921 tccgcaccgt tccagccttc ggtggcgaca ctcagaaagg gcaggtcaaa cttggccggg
     1981 tcgttgccgc gctggcgcag atgcacacgc agaatgtctt cgcgctcatg gtcattgggc
     2041 agatcaatga aaaagacctg atcaaaccga cctttgcgga tgatttcggc gggcaacagg
     2101 tcgatacggt tggcggtcgc ggccacgaag acctgattgc tcttttcctg catccaggtc
     2161 agaaaggccg aaaagatgtg ggaacccgta ctgccgtctt tggctgtcga agagctgatg
     2221 ccgccttcga tttcgtcgat ccagagaatg gccggggcga ccgcttcgat gatctgaatg
     2281 gcgcgatgga acgtgccttc cggacttccg tgcatgccgg aatacacctg gttcatgtcg
     2341 agcctgaaca ggggaaggtt ccagagggag ctgatggcct tgaccgatag cgacttgccg
     2401 cagccggaga tgcccatcat cagcagaccg cggggagggt gcagtccggc ggcctcggct
     2461 tcggggctga acagcttctt gcgctttacc agccactctt tgaggttctg cagaccgcct
     2521 acgtcttcga gcgacgtttg cgacctgtaa tattcgagaa ttccttcttt gcgtaccagt
     2581 tgttctttct cagaatgcag ctgcggaagt atttcggcgt tgatcgtttt gcgaccccag
     2641 aggaccttgt tgagagtgaa ctgagcttca gaagcggtga agccctgcag agcgaccgcg
     2701 gcctgagaga cttcatcggg gctcaactgg ttttctgccc cgcggcgggc gcagttctgg
     2761 gccgcgcgtt gcacgatctc ctgcagctcg ttcacgtcgg gcagggaaaa ctcgatcaga
     2821 tgaatatcct ggcgtagctc gactggcaac agaatctgcg taccgaccaa aaagagaaac
     2881 cggttctggc cgcgatactg cgtggcaaac gctttcagtc gcctctgcac ctctggctgc
     2941 ggaaggaaaa aggccagatc cttgaaaaga atcgcgccgc gctcacttgg ctgtgaaagc
     3001 aggtaatcaa gagccggaag caggccgatc gttccgtcga tcgtgggacg agcaccctcg
     3061 ggggtgatga cgcgtagtcc ctgctgcagg tcccaggtct ggaacgaacc ttcctgggcg
     3121 aaggccatgc gagaaatgct gtgaatcgcg ttctcggcct ggtcttcctc ggaggtgacc
     3181 agggcaataa cggggaaacc ggcgaccagc gctttcttga tggcattgac cgccgtcgtt
     3241 ctcgaggagg ttgccactcg tcagggaagg gtcagtcgat cgctggaagc gctcaggcgg
     3301 tggctgccgg cggtcggttg attaccactg gcgttgctct gagtgacgga ctgccgatac
     3361 agatctttct cgaacagatc ataggtgtag gcgaacccgg tcaccggatc atacagcgag
     3421 taactgcggg gggccatctc agtatcgtgt cttgttttca tggtactttt cccagatgag
     3481 aaaattgaag gtgaacacgt gccatcatac cccagatcag tcgggaatct caacaaaaac
     3541 gtcgtggaca ggaacaggta attgtgatat cttacccctc cttccctgaa cagggtgggc
     3601 cgatttacga tctatgcaga cgacaattcg tagaggataa gccatgaaac agtatttgag
     3661 tcttttcttt gtgatgtgcg gtgcattcgc ctgtctcatc gggccggtgt atgcttcaga
     3721 gccgagcatg ctgcaacaac ttgatgccgc tcagatccag gagttccagg gtcgggcaca
     3781 ctcagtcacc aagcagttgg cgctggtcaa tacgatcgaa cgcctgttgg attgcgcggc
     3841 gtcagggcgc atcaacgtgt ctctcatcgg gttggacggg gtacccacct gggtgcgcga
     3901 gatgtggcag cgccacgaac ctgacgcggt cgaagtgagc gtgtcctgct atgcaatggc
     3961 tctcgatgcc aaacagccgg cggcgcgcac cgatttttac aggtttcgct cgactcggac
     4021 caacggtgag attgttgagt cgctgctgca ggacattcgg aaggataaaa aggcagcggt
     4081 gatcttcacc ttcatcgatg gtgatccgtt cggtcacgac aaagagattc agcgtgtgct
     4141 gggggaactg ctggctgcca ttgaagaagg cgaagtggtg gtgaaaaacc tgaagctggc
     4201 tgatgccccc ctgcgagtga acaatctctg gcgtgccaac gccattccag gctacattcc
     4261 cgaacgcgtg ctggtacaac aatatct
//
LOCUS       DAORHD010000258         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22242_length_4268_cov_4.075481, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000258 DAORHD010000000
VERSION     DAORHD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22242_length_4268_cov_4.075481"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKO06_06750"
     CDS             <1..1021
                     /locus_tag="PKO06_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69380.1"
                     /translation="VFGEVLAGVINARIYVWEPLKQSPWSLSKDWTTLNLYGELAPFI
                     TDMMAANGRLVPLEQAAMITRSIESLRTYNQEFASLPKRRPYNLAYAQMIDQRQSEPW
                     SGFASADPLYAFFDRNAPEASRHAIPAPFNDCVPGAHSLRDLGAFLSACRDIGRTAYT
                     IEVESADQLWPELARRGLPWRDTLTLSGFVLVRSRDKRLILDRDLRYLGNGGLLLEEG
                     DIEIRAPLRPISETRRHHVLQVVALNGDIRLATGREALIQAHLLASGRLTFAGSEPVR
                     VRGGIAAGRLFDRPEEAETFPGGSLEYLPEMAAFPRGGESSVDTPDEKSLLSFSFEPA
                     PVIHD"
     gene            1014..1472
                     /locus_tag="PKO06_06755"
     CDS             1014..1472
                     /locus_tag="PKO06_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69381.1"
                     /translation="MTEQHHEKYPTGRPRGGLSYLEVVVALFVLMMALIPVFQVFTQG
                     TAGTMLTRDELMAQQYAGEMMAWAHARGFDGLAIEDWREPKEVRAIEESAPIDARFQR
                     WFSVRLKQPLGGLSDWPMEYKIIHVEIRWESSGKPQSLVQTGLLFRGRMP"
     gene            1469..2047
                     /locus_tag="PKO06_06760"
     CDS             1469..2047
                     /locus_tag="PKO06_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69382.1"
                     /translation="MNMTFRDRRHAVTVVELLVAAVIGAILSLAITKVFFSSVRVSQK
                     GMEHLNNVQNASIVLTQIDQDLRRADSLQLPPADLPGGLLQIAGADEQGLLTVVYQSS
                     PDGRGVMRRVSRVTAHSAADQTQSHLYCQGLPTTLTFEPVTINTCQTCRVSLRVSSAG
                     PGGEEIALKRLFYLPNLPDNHRRVTCDWTYGN"
     gene            complement(2100..4070)
                     /locus_tag="PKO06_06765"
     CDS             complement(2100..4070)
                     /locus_tag="PKO06_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69383.1"
                     /translation="MPAAHLTVWEPVIEANDSEAASRLLEGGATLTATDSCGRSIVHL
                     IACTGADRVFHAIRADLPLHDSPDAFGRLPLHDAALNGHAPLIDPLMNRCHGPSPTDQ
                     DGFTPLHEAVLGDHLAVVELLLARGAVVHLPNKRGILPLHLATSVPVIKTLVRAGADL
                     NATDHRRNTFRERLLACGPTTLAREVLAAFPPETEDEIASLLSTLPEFTLDFMSEWKT
                     FDRFSQPLKKAILFAAARYRKREVWTTLFWSGMGEDQHDEQGNSLLEVAAESGDTALV
                     ERLIESGFQPTPARTRAKTLLHLAAGSGNAETVEVLLQRGCDPLAQCGPGETPLWFAV
                     VKDAREVIPVLVKAGANPNLPGPNGEYPLFLAFTFGRTELIECLLNHGADPHVTDSYG
                     NTILHQAAHAKNGELVRVLLRHGADPMRKNKSGITAFEEIQKSCPVEVAMELLESLRA
                     KGVTIKGLRPLLGMVPDVSRADSLLQLGAQVQDVGDDGSTPLHMQAESGNLAMVSFLL
                     EHGASPTAARRDGATALHLAAAQAHPGVVERLIAARARVNATDTAGRVPLVYACEALK
                     ERDPLSSYTASQVYQARAFADPDSEKLDRIIRLLIDAGADLFVPVGERPFIEWARGKG
                     MARLSKTLAEILASARSSSTLVNRFSIWLKKR"
BASE COUNT          857 a         1221 c         1311 g          879 t
ORIGIN      
        1 tgtattcggc gaggtgctgg cgggggtcat caatgcgcgt atctacgtct gggaacccct
       61 gaaacaatcg ccctggtcgc tctcgaaaga ctggacaacg ttgaatctgt atggcgagct
      121 ggcaccgttc attacggaca tgatggcggc gaacggcaga ctggtgccgc tcgaacaggc
      181 tgccatgatc accagatcga tcgaaagtct gcgcacgtat aaccaggagt ttgcgtcgct
      241 accgaaacga cgaccgtaca atctcgcgta tgctcagatg atcgatcaga gacagtcaga
      301 gccctggtct gggttcgctt cggccgaccc gctctacgcg tttttcgatc gcaacgctcc
      361 ggaagcttct cgccacgcca ttccggctcc attcaacgat tgcgttcccg gagcgcacag
      421 cctgcgtgat ctgggtgcgt ttctgtcggc ctgccgtgac atcggccgca cggcctacac
      481 catcgaggtc gagtctgccg atcaactctg gccggaactg gcccggcgcg ggttgccgtg
      541 gcgcgatacg ctgacgctga gcggctttgt gctggtgcgc agtcgcgaca aacgtctgat
      601 tcttgatcga gatctgcgct accttggcaa cgggggcctt ttgctcgagg aaggcgacat
      661 cgagattcgg gctccgctgc ggccgatttc tgaaacgcgt cgtcaccatg tgctgcaggt
      721 ggtcgcactg aacggcgaca ttcgcctggc gaccgggcgt gaagccttga tacaggcgca
      781 tctgctggct tcgggacggt tgaccttcgc cggatcggaa ccggtgcggg tgcgcggggg
      841 gatcgctgcc ggacgattgt tcgatcgccc cgaagaggcc gaaacttttc cgggggggag
      901 tctcgagtat ctgccggaga tggcggcgtt cccccgcggc ggcgaatctt ccgtcgacac
      961 cccggatgag aaaagcttgc tcagtttcag tttcgagccg gcaccggtga ttcatgactg
     1021 aacaacacca cgaaaaatac ccgactggac ggccccgcgg cggtctcagt tacctcgagg
     1081 ttgtggtagc gctgttcgtt ctgatgatgg ctttgatccc ggtttttcag gtattcacgc
     1141 agggaacggc cgggacgatg ctgacccggg atgaattgat ggcccagcag tatgcgggcg
     1201 agatgatggc gtgggcgcat gcgcggggtt tcgatggtct tgcgatcgaa gactggcgtg
     1261 aaccgaagga agtcagggct atcgaggaaa gcgcgccgat cgatgcccgt tttcagcgct
     1321 ggttctcagt tcgattgaaa caaccgctcg gcggcttgag tgactggccg atggaataca
     1381 agatcattca cgtcgaaatt cgatgggaaa gctcaggaaa gccacagtca ctggtacaaa
     1441 ccggtctgct gtttcgaggt cgaatgccat gaacatgact tttcgagatc gtcgacatgc
     1501 cgtgaccgtg gtcgaacttc tggttgccgc cgtcatcggt gccattctgt cactggcgat
     1561 caccaaggtg tttttctcaa gtgtccgagt cagtcaaaaa ggcatggagc atttgaacaa
     1621 tgtgcagaac gcctcgatcg tgctcaccca gatcgatcag gatcttcgtc gagctgactc
     1681 tctgcagctc ccacccgccg atcttcccgg cggactgctg cagatcgccg gggctgacga
     1741 gcagggcctg ctgacggtcg tgtatcaatc gtctcccgac ggtcgcggcg tgatgcgtcg
     1801 cgtgagccgt gtcaccgctc attctgccgc agatcagacg cagtcgcact tgtactgtca
     1861 aggtttgccg actacgttga cctttgaacc ggtgacgatc aatacctgtc agacctgcag
     1921 ggtgtcgctt cgtgtctcgt ccgccgggcc cggtggtgaa gaaattgcgt tgaagcgtct
     1981 gttctatctg ccgaatcttc ctgataatca ccgccgcgtc acctgcgact ggacctacgg
     2041 gaactgaaaa cggcagcgcc tcatgacccg acctgagttg tcgtgccgac gctgagtcgt
     2101 cagcgctttt tcaaccagat cgaaaagcgg ttcaccaatg tcgacgagga acgggcactc
     2161 gcgagaatct cggccagtgt ctttgaaagc ctcgccatgc cctttcccct ggcccattcg
     2221 atgaacggcc gttcgcctac cgggacgaac agatcagccc cggcatcgat gagcaggcgg
     2281 ataatgcgat cgagtttctc ggaatccggg tcggcgaagg ctctggcctg gtagacctga
     2341 gaagcggtgt aggaactcag gggatcgcgt tctttcagtg cttcgcaggc gtagacgagg
     2401 ggaactcgcc cggcggtatc agtcgcattg acgcgggccc gggcggcgat gagtcgttcg
     2461 acaacacccg ggtgcgcctg cgctgccgcc aggtgtagag ccgtcgcacc atcacgtcgg
     2521 gcggccgtgg gtgaagcccc gtgttccagc aggaaggata ccatcgcgag attccctgac
     2581 tcggcctgca tgtgcagagg cgtcgaacca tcgtctccga catcctgtac ttgtgccccc
     2641 agttgcaaca gagaatctgc ccgactgaca tccggtacca tgccgagcaa ggggcgaaga
     2701 cccttgatgg ttacgccttt ggcgcgaagc gactcgagca gttccatcgc gacttcgacc
     2761 gggcaggatt tctgaatctc ttcaaaagcc gtgattccac tcttgttttt ccgcatggga
     2821 tcagccccat gacgtaacag cacccgtact aactccccat tcttcgcatg agctgcctga
     2881 tgcagaatcg tattcccgta ggagtccgtg acatgcgggt cggctccatg gttcaacagg
     2941 cactcgatca gttcggttcg accgaaggtg aacgccagaa acagcggata ttcaccattg
     3001 ggaccaggaa gattggggtt ggcccccgct ttgacgagaa ccggaatgac ctcacgggcg
     3061 tccttgacca ccgcgaacca gagaggcgtc tcacctggac cacactgagc cagaggatcg
     3121 cagccacgct gcaacaatac ttcgactgtc tcggcgttcc cgctgccggc cgcgagatgc
     3181 agcagtgttt tggctcgggt acgagctgga gtaggctgga agccggactc aatgagtctt
     3241 tcaacaagag ccgtgtcgcc cgactcggcc gcgacttcaa gcagactgtt cccctgctcg
     3301 tcgtgctggt cttcgcccat gcctgaccag aacagcgtcg tccagacctc gcgcttgcga
     3361 taccgggcgg cagcgaaaag aatggccttt ttcaacggtt gggaaaaccg gtcgaatgtc
     3421 ttccactcgg acatgaaatc gagcgtgaat tcgggcagcg tcgagagcag tgaggcgatc
     3481 tcgtcttccg tctcgggagg aaaagcagcc aggacctcgc gggcaagcgt tgtcggaccg
     3541 caagccagca ggcgttcacg aaaggtgttc cggcggtggt cggtcgcgtt gaggtcagca
     3601 cctgcacgaa ccagtgtctt gatgacgggc acggacgtgg ccagatgaag aggcaaaatg
     3661 ccgcgcttgt ttggcaggtg aacgacggct ccacgggcaa gcagcagctc gacaaccgca
     3721 agatgatctc ccagaaccgc ttcgtgaagc ggagtgaaac cgtcttgatc ggtgggactc
     3781 gggccgtggc accgattcat cagaggatcg atgaggggcg catgaccatt gagagcggca
     3841 tcatgaagag gcagacgtcc aaaggcgtct ggtgaatcat gcaatggaag atcggcgcga
     3901 atggcgtgaa acacgcgatc ggcacctgta catgcgatca ggtgcacgat cgatcgaccg
     3961 cacgaatcgg tggcggtcag cgtcgcccca ccctcgagca gacgtgaagc ggcctcgcta
     4021 tcgtttgctt cgatcactgg ttcccagacg gtcagatgtg cggctggcat gatgacgagt
     4081 atactgcaac ccctgtgttg ggcaaaaggg agcgaatcgc tgacgcagtg ggtatcaggg
     4141 ctcacaggaa ggacgacacg cgccgtgcgt ccgtgtgctc gtcactcacc gaaagagtcc
     4201 ccgttctctc tgaacgcggc tgttggcgcg ttatcagtct gaacgtcggt gtgttaccat
     4261 tcgtggcg
//
LOCUS       DAORHD010000259         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22248_length_4267_cov_4.810779, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000259 DAORHD010000000
VERSION     DAORHD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22248_length_4267_cov_4.810779"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="PKO06_06770"
     CDS             complement(<1..960)
                     /locus_tag="PKO06_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69384.1"
                     /translation="MQSCIGRLSQFALACLCIWGVQVAEAQDYDQFKSFARRDGTVDD
                     ILKGPQNRPRAETYYLIALSQARKDKVRDALTTIERGLSLEPGNTRLMSLRAALWART
                     GRTMDAIAEFERVLAIQPDDKYARESLLQLVPSPRPTRMPTLPANVPDAPVRVDRPSA
                     SGTQALPDRPEPAKVLEAKYFTAMKLKQRCFFQLSALRRALGTGNKSASGTLDLKALV
                     AGGKLPSEPVCPENGVYSLKGDEPVCSSHGPLSQVEVEVNALYTDFNRGMTAKTRGNL
                     TEARQAFDQVVILYPRWSEAYYQLADTLFRMGEETLALEKIKTC"
     gene            complement(966..3383)
                     /locus_tag="PKO06_06775"
     CDS             complement(966..3383)
                     /locus_tag="PKO06_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69385.1"
                     /translation="MPYCPGCGAVNQEEALNCGACGRVLGTICGHCHSPNQMGARYCT
                     GCGKLMGTATKDRLGAPEGEPIAEMMARKRPVVVAPGTTEVPPRAAFLKVVLGGFIFA
                     LFYLSQALSGYPILTILVGLGSGVVCLWGLVDLALWVMERGETHTAGGATEEEILEPL
                     PETALSEAVVAEGSFADAERDLEPVTRVATPTATTPSPLPFTESSPVAPTTTSEVAPP
                     APSDVLSSREDDLTVSQEDMLEARERAKSSQTLAEFLEQGVVHEIRSTERKLDKSPKN
                     VSLLMRVAQLYEERGEMSNALSAMEHCIKLDPREPEIYLYHGVLLRRAGRVDEARQAL
                     EKAIGLNRFLSKAFYQLGALERSQNRLQPARDAFQRCIQLAPDDAYAHYQLGMIYRDM
                     NDLGLAQMELKRACLLNPHDSYGHSRLGQIYQLNKQWEQAVYEFSQALSLKPNDAFVL
                     EKLAEVMVEKNELERARDLYQEALSHQFHPEPSTMVAQARVLYKLGRTAAMKPILDEI
                     LRLAPDQPDALYLYALVRSSEKDIDGAIDLLKKVTTLHPERWEVWFELGRLLQSLDRN
                     EEALAAYIKASPNASDQAGIWNTIGVLLTNQKDFEGATKAFKKAVSFDYSDPQIQANL
                     RAVQKKVESTCRRIIEKANELLAANPNELSAYIELGHAYELLDRPEDALMSYQRLLSI
                     KSDSIEGLAAYARLLKSRGKMRMAMRCYREILKLQPNHFDARLQLVRTGLNLGHINEA
                     LRHAVVAQKLHPDDPQVHFLLGKIYFAKGLAPRALKEFTQVANTARDPDMVSWAEIMR
                     RRLTKNL"
     gene            complement(3392..3784)
                     /locus_tag="PKO06_06780"
     CDS             complement(3392..3784)
                     /locus_tag="PKO06_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69386.1"
                     /translation="MSVIRLILLDSRDAVSGLIPSRSLSVVIWALAEGGRTHESFWKK
                     VATLDAELAAYFKHHQDPEPLLEGQGDGLLVVNWEHRCIESFQEYQPVRREGVALEHN
                     GLHRVDGAGHPYRLGDEWHLVDHQFEEG"
     gene            complement(3781..4176)
                     /locus_tag="PKO06_06785"
     CDS             complement(3781..4176)
                     /locus_tag="PKO06_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69387.1"
                     /translation="MNLFESLRISNRLLVYAMMSTLVVAFGCLGTLIIGYFEPNLAIL
                     GLLLGSVFGVFIALHESSVVGSLIGMLAGMILGCFVYLLIDFETAYLTVFVLSLFGAF
                     MGEPFAYFWREANRIGEDLGPTTVSRRAT"
BASE COUNT          887 a         1288 c         1219 g          873 t
ORIGIN      
        1 gcaggtcttg atcttctcga gagcaagcgt ttcctcgccc atgcggaaca aggtatccgc
       61 cagctgatag taggcttcag accagcgtgg atacagaatg acgacctggt cgaacgcctg
      121 ccgggcttcc gtcagattcc cccgggtctt ggcggtcatc ccgcgattga agtcggtgta
      181 caaggcgttt acctcgacct caacctgcga caatggtccg tgcgatgagc agacgggctc
      241 atcacctttc aggctgtaga cgccattttc ggggcacacc ggctcggacg gaagttttcc
      301 gccggccacc agcgctttca gatcgagcgt gccgctcgcc gatttgttcc ctgtccccag
      361 cgcacgccgc aaggccgaca gctggaaaaa gcagcgttgc ttgagtttca tggcagtgaa
      421 gtatttcgcc tcgagcacct tcgcgggctc aggccggtca ggcaaagcct gcgttccaga
      481 cgccgaaggc cggtcgacgc gaacgggagc gtcgggcaca ttggccggca acgtgggcat
      541 gcgcgttggc cgcggcgacg gcaccaattg cagcagagac tcgcgcgcgt atttatcgtc
      601 gggctgaatg gccagcactc gctcaaattc cgcgatggca tccatcgtgc ggcctgttcg
      661 agcccagagc gcggcgcgca gactcatcag gcgagtattg ccaggctcga ggctcagacc
      721 tcgctcaatg gtcgtcaggg catcacgcac cttgtccttg cgcgcctgtg acagggcgat
      781 gagatagtag gtctcggcac gcgggcgatt ctgtggcccc ttgagaatgt catcgaccgt
      841 tccatctctg cgcgcaaaac tcttgaactg atcatagtcc tgcgcctcag caacctggac
      901 accccagatg cagagacatg ccagagcgaa ctgactcaac cggccgatgc agctctgcat
      961 gagagtcaca ggttcttggt cagacgacgg cgcatgatct cggcccaact gaccatatcg
     1021 ggatcacgag ccgtgttcgc cacctgcgta aactccttga gcgcccgcgg agccagccct
     1081 ttggcaaagt agatcttgcc gagcaggaag tggacttgcg gatcgtcggg gtgcagcttc
     1141 tgcgccacga ccgcatgacg caacgcttca ttgatgtgac ccaggttcaa acctgtacgt
     1201 accagctgca ggcgagcgtc aaagtgattc ggctgaagtt tcagaatctc gcgataacaa
     1261 cgcatggcca tgcgcatctt gccgcggctc ttcagcagtc gggcataagc tgccagacct
     1321 tcgatcgagt cagacttgat ggaaagcagt cgttgatacg acataagcgc atcttctgga
     1381 cgatcgagca gttcgtaggc gtgtcccagt tcgatatagg cggaaagctc gttcggattc
     1441 gccgccagca gttcgttggc cttctcgata atgcgccgac aggtcgactc gaccttcttc
     1501 tgaaccgcgc ggagattggc ctgaatctgt ggatcggaat aatcgaaact gaccgccttc
     1561 ttgaaggctt tcgtcgcccc ttcaaaatcc ttctggttcg tcagcagcac gccgatggtg
     1621 ttccagattc ccgcctgatc gctggcgttc ggggaagctt tgatgtatgc cgccagtgcc
     1681 tcctcgttgc gatcgagcga ctgcagcagt cgtccgagtt cgaaccagac ttcccagcgt
     1741 tcgggatgga gtgtcgtcac ctttttcagc agatcgatcg cgccatcgat gtctttctcg
     1801 gaggagcgaa ccagcgcata cagatacagc gcgtcgggtt ggtccggggc caggcgcaga
     1861 atctcgtcga gaatcggttt catcgcggcg gtgcgcccga gtttgtacaa gacacgagcc
     1921 tgcgccacca tggtggaagg ctcaggatga aactgatgcg acagcgcttc ctgatacaag
     1981 tcgcgggccc gttccagctc gtttttctcg accatgactt cggccagctt ctcgagcacg
     2041 aacgcatcgt tcggtttcag gctgagcgcc tgcgagaact cgtacacggc ctgttcccac
     2101 tgtttgttca gctgatagat ctgtcccagc cgactatgac cataggaatc atgcgggttg
     2161 agcagacagg cgcgtttcag ttccatttga gccagaccca gatcattcat gtcgcggtag
     2221 atcatgccca actggtagtg agcgtaggcg tcatccggag ccagttgtat acagcgttga
     2281 aatgcatcac gcgcgggctg caggcgattc tgcgatcgtt ccagcgcgcc gagttgatag
     2341 aaggctttcg acagaaagcg gttcagtccg atcgctttct caagcgcctg ccgcgcttcg
     2401 tcgacacgac cggctcgacg cagcaggaca ccatgataca gatagatttc cggttcacgc
     2461 gggtcgagct tgatgcagtg ttccatcgcg ctcagcgcgt tgctcatttc accgcgttct
     2521 tcatacagct gcgccacccg catcagcaga gacacattct tcggtgactt gtcgagtttg
     2581 cgttcggtac tgcgaatctc gtgcaccaca ccctgctcga gaaactcggc aagggtctgc
     2641 gacgacttgg cgcgctcacg cgcctcgagc atgtcttcct gtgaaaccgt cagatcgtct
     2701 tcgcgagatg aaagtacatc actcggagcg ggcggggcaa cctctgacgt tgtcgtaggc
     2761 gcgaccggtg acgattccgt gaatggcaga ggagacggtg ttgtggcagt tggcgtcgcc
     2821 acacgggtca cgggttcgag gtcacgttcc gcatccgcaa acgacccttc ggcaaccacg
     2881 gcctcgctga gtgcggtttc gggaaggggt tccaaaatct cttcttcggt cgcccctcca
     2941 gccgtatgag tttcgccccg ttccatgacc cagagggcca gatcgaccag accccagagg
     3001 cacaccactc cgcttccgag gccgacaagg atggtcagaa tcggataccc gctcagtgcc
     3061 tggctgagat agaacaacgc gaagatgaag cctccgagaa ccactttgag aaacgccgcg
     3121 cgcggtggga cttccgttgt accaggagca accaccacgg gtcgttttct ggccatcatc
     3181 tcggcaatcg gttctccttc gggagcaccc aggcggtctt tggtcgccgt acccatcagt
     3241 ttgccgcaac ccgtgcagta tctcgctccc atctgattgg gcgaatggca atgtccgcag
     3301 atcgtgccca gcacgcgccc gcaggccccg caattcaggg cttcttcctg gttcaccgca
     3361 ccgcaacccg gacaatatgg catgtcggtt ctcaaccttc ctcaaattgg tgatcgacca
     3421 ggtgccactc gtctcccagt cgataggggt gtcctgcgcc atcgacgcgg tgcaggccat
     3481 tatgttccaa cgccacgcct tcgcggcgta ccggttggta ttcctgaaat gactcgatgc
     3541 aacggtgttc ccagttcacg accagcaagc catcgccctg cccttcgagc agcggttccg
     3601 gatcctgatg atgcttgaag taggcggcaa gctcggcatc gagcgtcgcg acttttttcc
     3661 agaagctctc gtgcgtgcgc ccgccttccg ccagagccca gatgacgact gacaagctgc
     3721 gtgagggaat caggccggag accgcatctc ggctgtcgag caggatcaga cgaatgacac
     3781 tcatgtcgcc cgtcgtgata cggtggtcgg ccccaggtcc tcgccgatgc gattcgcttc
     3841 gcgccagaaa taggcgaagg gctcacccat gaacgctccg aaaagagaca acacaaacac
     3901 agtcagatag gccgtctcaa aatctatcaa caggtacacg aaacagccga gaatcatgcc
     3961 ggccagcatg ccgatcagac tgccaaccac actcgactcg tgcagggcaa tgaaaacacc
     4021 aaacacggaa ccgagcagca gaccaagaat cgccagattc ggctcgaaat agccgatgat
     4081 caatgttccc agacacccaa acgccacgac gagcgtgctc atcatggcgt agaccagcag
     4141 gcgattcgaa atacgcagcg attcaaacag gttcatgggg ttcgaggtag cgtctcagtc
     4201 ggaaagaaac cggcgagccg ccgatatcgc ggctcgccgt ggtcggtcgt tcacggggtg
     4261 agaatgg
//
LOCUS       DAORHD010000260         4265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22267_length_4265_cov_3.785986, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000260 DAORHD010000000
VERSION     DAORHD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22267_length_4265_cov_3.785986"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKO06_06790"
     CDS             complement(<1..406)
                     /locus_tag="PKO06_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69388.1"
                     /translation="MAERIGRSAAVKPQTHCWLGASAAERFGAGLIVWFLLLVWMPVW
                     AQSPLKVALVGALTGPKAVYGLSHRDGAHLAAEMIGSRSISLIDIDDAGDSGKVGPFV
                     VDAVMRQQVHALMGSVDSGCTHVLAMVAVKLHV"
     gene            complement(373..1200)
                     /gene="murI"
                     /locus_tag="PKO06_06795"
     CDS             complement(373..1200)
                     /gene="murI"
                     /locus_tag="PKO06_06795"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNV69389.1"
                     /translation="MDDRPIGVFDSGVGGLTVVKEVRHLLPHEDIIYFGDTARVPYGN
                     KSIPVIRRFCLEIVRFLEEMRVKMIVVACNTASALALDFIRRQTSLPVVGVIDPGVRS
                     ALMESRDGVIGVIGTVATIRSEAYQSRLRRLKSGVRVHAKACPLLVPLVEENLLDAAI
                     TRQALEMYLSELRDLSLETLILACTHYPLLKTLIADFFGPSVKLIDSAQETAREVSEV
                     LKARRIAAGNDRTGSEEFYVSDSPDSFAAVGAPFLGRDMRRVFLEPVWQRELVVPPL"
     gene            complement(1255..2304)
                     /locus_tag="PKO06_06800"
     CDS             complement(1255..2304)
                     /locus_tag="PKO06_06800"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV69390.1"
                     /translation="MGGTWQAFDKCRSRAGLFLSICGVVSLLMLVGCDSAGTPAKIDK
                     KLKIGLLITPKGLNDKGFNDLAYTGLKSAERKYGIEPTVIEPSTMKDQEACLRFFAAQ
                     KLDAVIAVGVAFLDSIRRVASENPQLRFLVIDSDVSEGQIQGVAFREYEASLLCGFLA
                     SARSKTGKIGFIGGVDIPVIRRFLVGYEQGAKLASPSIEVVTQFVGEDFSGFNKPDRA
                     KEIAHEMYQNGCDVVFPAAGASGLGVISAAVQNKKYVIGVDMNQDSLAPGLVLTSLLK
                     RVDTVVEDIVKTLAEGKGNESVKKTYGLADGGVDLTDFQFSRQELGVELINRVNELRQ
                     EVIRGKVGSFSTNLH"
     gene            complement(2247..3662)
                     /locus_tag="PKO06_06805"
     CDS             complement(2247..3662)
                     /locus_tag="PKO06_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69391.1"
                     /translation="MSVPVAGRLNIALIFPGPYALGISNLGYQTVHRLMGSVPGIGVE
                     RFFFPILAGQPHPPPFYSFETRRPLGDFDVLAFSISYEGDFDRLPDILGPLGIPIMAE
                     KRGRHHPLLIAGGAAIGANAAAVSRVFDVLIPGEAETTLPHVLQRLLEQGPSAEVVAD
                     LPGVWVPRCRVQVTDTSHPYDMNILPAYSHLVSPENVFQGARLIEVMRGCPRTCAFCL
                     ARVLYHPCRWLTHERFEWCLDTLVPADRLGLVAPSLFDHPHLVQILESLAARRIRLHN
                     SSVKWEKLTAEVLNLLGRCGVKSLTLAPETGSAQLAASMGKTLLQERFFATVDRCADQ
                     GIERLKLYFMIGLPDETAEDLEQTLAFVRTCAERLSGRMQGLSVSFSGFVPKRRTAWE
                     AKTVCDAGKLKKLFQAVRRSLAELAPFVKFHAETPAQVLRQAYLATVGPELAEELELE
                     AKLWEARGRRSISADLEQDYS"
     gene            complement(3819..>4265)
                     /locus_tag="PKO06_06810"
     CDS             complement(3819..>4265)
                     /locus_tag="PKO06_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNV69392.1"
                     /translation="MGIGIAQGENRAVDAAQMAINSPLLESSIAGAKGVLINITAGPD
                     LTLHEVNKACSIVEETVDQDATIIFGAVYDENLKGSLQIAVIATGFGEEARNAVDPKR
                     KIIEDLRTIRTQEIAQPVAAAHSNVTPIAASLDDREIPAFLRRKRG"
BASE COUNT         1007 a         1331 c         1113 g          814 t
ORIGIN      
        1 gaacatgcag tttaaccgcc accatcgcca gcacatgcgt gcagccgcta tcaacgctac
       61 ccatgagcgc atgtacctgc tgtcgcatca cggcatcgac cacgaacggt ccgactttgc
      121 cgctatcgcc agcatcgtca atgtctatca ggctgatcga gcggctacca atcatctctg
      181 cggctaaatg tgcgccatcg cgatgcgaca aaccatagac cgctttcggc ccggtcagcg
      241 caccgacgag agcgaccttc agtggagact gtgcccaaac cggcatccaa accagcagca
      301 aaaaccagac aatcagaccc gcgccaaacc gctcggctgc cgatgcgccg agccaacagt
      361 gcgtctgggg tttcacagcg gcggaacgac caattctctc tgccacacgg gctccagaaa
      421 cacccgtcgc atatcacggc cgagaaatgg tgcgccaacc gccgcgaagg aatcaggact
      481 gtcagagaca tagaactctt cagaaccggt acgatcgttt ccggcggcaa ttcgtcgcgc
      541 tttgagaacc tctgaaacct cacgcgcggt ttcctgagcc gaatcgatca atttgaccga
      601 cggcccgaaa aaatcggcga tgagcgtctt gagtaacgga taatgagtac aagcaagaat
      661 caacgtctca agcgacaggt cacgcagttc agacaggtac atttcaagtg cttgccgcgt
      721 gatcgccgca tcgagaagat tctcttctac aagtggcacc aacaaaggac atgccttcgc
      781 atgaactcgg actcccgact tcaaacgacg caggcgagat tggtatgctt cagatcgaat
      841 ggttgccacc gtgcctataa caccgatgac cccatcacga ctttccatga gcgcggatcg
      901 tactcccgga tcgatgaccc cgaccaccgg cagcgaggtc tgccgacgaa taaaatcgag
      961 cgccagcgcc gaagccgtat tacaggccac gacgatcatc ttcacgcgca tctcttcgag
     1021 gaaacgaaca atttcgagac agaatcgtcg aatgaccgga atcgacttgt tgccgtaggg
     1081 cacccgcgcg gtatccccga agtagatgat gtcttcatgc ggcagcaggt ggcggacttc
     1141 tttgacaacc gtcagccctc cgactcccga gtcaaacacc ccgatgggcc tgtcatccat
     1201 tgctgcacct cttcgctcga caccgacatg cgaagccgtc ggcgtgccgc aacctcaatg
     1261 aaggttggtg gaaaacgaac ccactttgcc gcgaatgacc tcctgtcgca gctcgttgac
     1321 gcgattgatc aattcgacac ccagttcctg acggctgaat tgaaagtccg tcagatcgac
     1381 tccaccgtcc gccaacccgt aggtcttctt cacggattca ttgcccttgc cttccgccag
     1441 ggttttgacg atgtcctcga cgaccgtgtc cacacgcttc aacaggctgg tgagtaccag
     1501 cccaggggcc agagaatcct ggttcatatc gacaccgatg acatacttct tgttctgcac
     1561 cgccgccgaa atcacgccga gccccgaagc tccagccgca ggaaacacga cgtcacagcc
     1621 attctgatac atttcgtgcg cgatttcctt cgcccggtca ggcttgttga agcccgaaaa
     1681 gtcttcgcca acgaactggg tcacgacctc aatacttgga ctggcgagct tcgctccctg
     1741 ctcataccct acgagaaagc ggcgaatgac cggaatgtcg acgccgccaa tgaaaccgat
     1801 cttcccggtc ttcgagcgcg ctgacgcgag aaagccgcac agcaggctcg cctcgtattc
     1861 gcgaaaagcc acgccctgaa tctgcccttc ggacacatcg ctgtcgatga caagaaaccg
     1921 caactgcggg ttttctgagg ccacgcggcg aatactgtcg agaaatgcga cgccgacagc
     1981 aatgaccgca tccagtttct gggcggcaaa gaagcgcaga cacgcttcct ggtctttcat
     2041 ggtcgagggc tcgataaccg tcggttcaat accgtattta cgctcggcgg atttcaaccc
     2101 ggtgtaagcc aggtcattga accccttgtc gttcaaacct ttgggtgtga tcaacagccc
     2161 gattttcagc ttcttgtcaa tcttcgccgg tgtcccggca ctatcgcacc ccaccagcat
     2221 cagaagcgac accacgccgc agatgctcag gaataatcct gctcgagatc ggcacttatc
     2281 gaacgcctgc cacgtgcctc ccataacttt gcctccaatt ccagttcttc cgccagttcc
     2341 ggcccgacgg tcgccagata ggcctgtcgc aggacctgcg caggcgtctc cgcgtgaaac
     2401 ttcacgaagg gcgccaactc cgcgagactt cggcgcaccg cctgaaacaa cttcttcagc
     2461 ttgcccgcat cacacaccgt cttggcttcc catgccgtac gccgcttggg tacaaaaccc
     2521 gaaaacgaca ccgacaaccc ctgcatacgc ccgctgagcc gttcggcaca cgtgcgtaca
     2581 aacgccaatg tctgctcgag atcttccgcc gtctcatcgg gtaacccgat catgaaatag
     2641 agtttcagcc gctcgattcc ctgatcggca cagcggtcta cagtagcaaa aaaccgctcc
     2701 tgcagcaagg ttttccccat gcttgccgcc agctgtgcac tccccgtctc cggagccagg
     2761 gtcagactct tcacaccgca acgccccaac agattcaaca cctccgcggt cagtttttcc
     2821 cacttgaccg aactgttgtg caggcgaatg cgacgtgccg ccagactttc gagaatctgt
     2881 accaggtgcg gatgatcgaa cagtgagggg gcaacaagac cgagacgatc ggccggaacc
     2941 agcgtgtcga gacaccattc gaagcgctca tgcgtcagcc agcgacatgg gtggtacaga
     3001 acacgagcca gacagaacgc gcaggtgcgc ggacagccgc gcatgacttc gatcaacctg
     3061 gcaccctgaa agacgttttc gggtgaaact agatgagagt atgcaggtag aatattcatg
     3121 tcgtaaggat gtgacgtgtc agtaacttgt acacgacaac gaggcaccca cacacccggc
     3181 agatcagcga caacctcggc acttggcccc tgctcgagaa gccgctgtaa cacgtggggc
     3241 agtgtcgttt cggcttcacc gggtatcaga acgtcgaaca cgcgcgacac ggcagccgcg
     3301 ttggcgccga tcgccgctcc gccggcgatg agcagcggat gatgtcgacc gcgtttctcc
     3361 gccatgatcg gaattcccag cggtccgaga atgtccggca gacgatcgaa atccccctca
     3421 tagctgatag agaaagcgag tacgtcgaaa tcgccgagtg gtcgacgcgt ttcgaacgaa
     3481 tagaacggag gcggatgcgg ttggcccgcc aggatgggaa agaaaaaacg ctcaacccca
     3541 atgccaggca cagagcccat caatcgatga actgtctggt acccaagatt cgagataccc
     3601 agcgcatacg gaccgggaaa aatcagcgca atgttgagac ggccggcaac cggaaccgac
     3661 acagagcaca cttcactctg atggagcgtg tcaacaacgg actcaaaatt ccagcgatgt
     3721 cgaatgccca cgatcgtcca ggttcagaaa ggaaaaacgt ctgcctcgac gccaggcgtc
     3781 gaggcagacg tttcggtcag caggaaaagg aaccgttctt atccgcgctt gcggcgcaga
     3841 aaggcgggaa tctcacgatc atcgagcgag gccgcgatcg gggtcacgtt ggaatgcgcg
     3901 gcagccaccg gctgagcgat ttcctgcgtg cgaatcgtgc gcagatcttc gatgatcttt
     3961 ctcttcgggt caaccgcgtt gcgggcttct tcaccgaaac cagtcgcgat gacggcgatc
     4021 tgcagcgagc ctttgagatt ctcgtcatac acggcgccga aaatgatggt ggcatcttga
     4081 tcgaccgttt cttcgacgat cgaacaggcc ttgttcactt cgtgcagggt caggtcgggg
     4141 ccagccgtaa tgttgatcaa cacgcccttg gcaccggcga tcgatgattc gagcaacggg
     4201 ctgttgatcg ccatctgcgc ggcgtcaacg gcgcgatttt cgccctgagc gataccaata
     4261 cccat
//
LOCUS       DAORHD010000261         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22295_length_4262_cov_3.947469, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000261 DAORHD010000000
VERSION     DAORHD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22295_length_4262_cov_3.947469"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PKO06_06815"
     CDS             complement(<1..497)
                     /locus_tag="PKO06_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV69393.1"
                     /translation="MTTQPLLSLPFETFKTILTDELGLEPYRAKQVWDWLFLRQASRF
                     EQMTNLSKEARAKLSERFPLIFPVFVDQLRSKDGTRKVVIPVGENELVEAVAIPGDED
                     SDGVTYCLSCQVGCQAGCTFCRTGAMGFKRNLTREEIILQVLSLIRVTGKKPTNIVFM
                     GMGEP"
     gene            complement(597..1982)
                     /locus_tag="PKO06_06820"
     CDS             complement(597..1982)
                     /locus_tag="PKO06_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV69394.1"
                     /translation="MKGKALPSQIKIVGLVLIICLVPIIAADYLLFQVKKMASTRSLL
                     LEIKHLPELEKTQLEIAEDLRAKAPALEKLQSDLQRLERLTTTYFIGSLVALGLGLAG
                     LYAFLSYLANPIQETLAKLRRFLNEDAYLKEFDVFSSSTEFDRFLQTSKNLLSENYSL
                     SLELKKKIEPLTNMAAFSDTNMNTFFTNVQEIFRSSNYIANTLESTTAAIQEVSTSAQ
                     TIADRSAKAAKNSDEASKIADGGLGAVTTTIETMESIKDEVFGLENIIEDLNSVSKQI
                     GEIVDTITNIAYQTNLLALNAAIEAARAGEHGQGFTVVAEEVRKLAEESGEAAEDIGK
                     KIRGMLQKTGIAVQTISKGTTKVVEGVNVAKVAGETLKRIVREVQTVNNAIQEISKAS
                     SEQSANVDSLRQSIESISGATRITSEGTKRVASAVQEQLNYIREYVGSTRELLTLVQL
                     LSDMLEKFNIK"
     gene            complement(2097..2699)
                     /locus_tag="PKO06_06825"
     CDS             complement(2097..2699)
                     /locus_tag="PKO06_06825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV69395.1"
                     /translation="MIDTSFPRLFADPDCFRHLVNQLQNGGIVALPTDTLYGLAVDGA
                     SRTGVEAIYRLKGRDENKPLILFLADLEALDLLPLTISAESRLLLHQHWPGALTAVFP
                     FAKDPRLTAFSFPGLGIRIPDHDDLRQFLREYPGFLLTTSCNRSDTPPLATVGEIRRE
                     FGRELAGIIDGGELRGGVPSTVVDFSQTPPRTLRQGKIQL"
     gene            complement(2696..3568)
                     /gene="prmC"
                     /locus_tag="PKO06_06830"
     CDS             complement(2696..3568)
                     /gene="prmC"
                     /locus_tag="PKO06_06830"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV69396.1"
                     /translation="MSVRDARLTLRTRFASAGIPDAEISADSLLELVLAASPGSLIRH
                     LRDQLTSEQHDQLETLARRREQREPLQYLLGTWGFLDLHLRVAPGALIPRPETEDWLL
                     ELFDFLPGTDWPSSFQFVDVGTGTGAIGLSIAHRFREARGLLIDVSRQALGVAQRNLE
                     RHPAEAERIHLCQADLTSPCRDRSVHLIVSNPPYIDSTELQGLMPEVRDYEPSLALDG
                     GKRGLEIINRLLGDACRVLKPGGFIALEHGMGQRFDILELAPASLRLVRTLDDAGKRD
                     RAIVWNYVPHTGNP"
     gene            complement(3577..>4262)
                     /locus_tag="PKO06_06835"
     CDS             complement(3577..>4262)
                     /locus_tag="PKO06_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014554116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNV69397.1"
                     /translation="FRMYTKYADGRRWKWEVMSRNDTGIGGVKEVIFSVDGQDVFGRL
                     KYESGVHRVQRVPETEASGRVHTSAATVAVLPEAEEVDIQIDEKDLRIDVYRSSGPGG
                     QSVNTTDSAVRVTHVPTGLVVACQDEKSQHKNKAKALRILRARLLDLANAKADKERAD
                     NRRSQIGSGDRSERIRTYNFPQQRLTDHRINLTIYRLSAVMEGELDEIIHALIEADNV
                     RKLAAEQIA"
BASE COUNT          901 a         1148 c         1254 g          959 t
ORIGIN      
        1 aacggctcgc ccatgcccat gaagacgatg ttcgtcggct ttttcccggt gacgcgaatc
       61 agactgagca cctggagaat gatttcctcg cgagtcagat tccgtttgaa ccccatggca
      121 ccggtgcgac aaaaggtgca gcctgcctga caaccgacct ggcaagacag acaataggtt
      181 accccgtcac tgtcctcgtc acctggaatt gccacggctt cgaccagttc gttttctccg
      241 acgggaatca cgacttttcg ggtgccgtct ttcgagcgca gttgatcaac gaacaccgga
      301 aagatcagag ggaagcgctc tgacagcttg gctcgagctt cctttgacag gttcgtcatt
      361 tgctcgaaac gactggcctg gcgcagaaag agccagtccc agacctgttt tgcgcggtag
      421 ggttcgaggc cgagttcgtc ggtcagaatg gttttgaacg tttcgaaggg caaggacagt
      481 aagggttgtg ttgtcatggc ttcctcagga aggaacatcg gcagaaagat gccgggggat
      541 taacgcgatc tgccgaccat ggccggaatg ggccctggag gccggcggac aagcggctac
      601 ttgatgttga acttttcgag catgtctgac agcaattgca caagcgtcag cagctcacgg
      661 gtcgatccca catactcgcg aatgtagttc agctgttcct gcacggcgct ggccacccgt
      721 ttggtgcctt cggaggtgat gcgggtcgca cccgagatgc tctcgatgct ttggcgcagc
      781 gaatcgacat tggccgactg ctcgctcgag gctttgctga tttcctgaat cgcgttgttg
      841 accgtctgaa cttcgcgaac aatacgcttc agggtttcac cggcgacctt ggcgacattc
      901 accccttcga cgaccttggt cgtgcctttt gagatggtct gcaccgcaat gccggttttc
      961 tgcaacatgc cgcgaatctt cttgccgata tcttcggcgg cttcacctga ctcttcagcc
     1021 agtttgcgca cttcctcggc gacgacggtg aagccctggc cgtgctcgcc ggcgcgagcc
     1081 gcttcaatag cggcgttcag agccagcagg ttggtctgat aggcgatgtt cgtaatcgtg
     1141 tcgacgattt caccgatctg tttactgacg ctgttcaagt cttcgataat gttctcgaga
     1201 ccgaacactt cgtccttgat cgattccatg gtttcgatgg tggtggtcac ggcgccaagg
     1261 ccgccgtctg cgattttcga cgcctcgtcg ctgtttttgg ccgccttcgc tgagcggtcg
     1321 gcaattgtct gtgccgaggt cgagacttcc tgaatggccg ccgtggtcga ctcaagggtg
     1381 ttcgcgatgt agttcgaact gcgaaagatt tcctgcacat tggtgaagaa tgtgttcatg
     1441 ttggtgtcgg aaaatgcggc catattggtc agtggctcga tcttcttctt cagttcgaga
     1501 ctgagagagt agttttccga cagcaggttc ttcgaggtct gaagaaagcg atcaaactcg
     1561 gttgagctgc tgaaaacatc gaactctttc aggtacgcgt cttcgtttaa aaatcgtcgc
     1621 aacttggcca gtgtttcctg gatcgggttg gccagatacg agagaaatgc gtacaggcct
     1681 gcgagcccga ggcccagtgc cacgagcgat ccgatgaaat acgtggttgt caggcgctcg
     1741 agacgctgca gatcgctctg cagtttttcc agtgccggtg cttttgcccg caagtcttcg
     1801 gcaatctcaa gttgcgtctt ttcgagttcg ggaaggtgtt tgatctcaag aagaagactg
     1861 cgcgtcgagg ccatcttctt tacctgaaag agcagatagt ccgcggcaat gattggtacc
     1921 agacaaataa tcagtaccag gccgacgatc ttgatctgcg acgggagagc tttacccttc
     1981 atgattcagc agcaacctga gatgaggcga agatgacagt cttctcggaa atgctccatc
     2041 ggcagttctc cgaagatgac tgagagtgca ttgtatcaca gccgttgcct caacgctcag
     2101 agctggattt ttccctgtcg cagagtgcgt ggaggggttt gcgaaaaatc gacgacggtt
     2161 gaaggcacgc caccacgaag ttcgcctcca tcaatgattc cagcgagttc tcgcccgaac
     2221 tcccggcgaa tttcgccgac cgttgcgagc ggcggggtgt cagaacggtt gcagctggtg
     2281 gtgagcagga accccggata ctctcggaga aactgcctca ggtcgtcatg atcgggaatg
     2341 cgaatgccca aaccgggaaa ggaaaatgcg gtgagacgag ggtctttggc aaaaggaaac
     2401 acggccgtca gagctccggg ccaatgttgg tgcagcagaa gtcgcgattc ggctgatatc
     2461 gtgagcggca gaaggtcgag agcttcgaga tccgcgagaa acagaatcag tggcttgttt
     2521 tcgtcacgcc ccttcagacg atagatggct tcgacaccgg tccgtgacgc tccgtcgacg
     2581 gccaagccgt agagagtgtc agtgggcagg gcgacaatgc cgccgttctg cagctggttg
     2641 accaggtgac gaaaacagtc cgggtcggca aagagacgag gaaaagaggt gtcgatcatg
     2701 ggttgcccgt gtgtggtacg tagttccaga cgatggcgcg gtcacgtttt ccagcatcgt
     2761 cgagggtccg caccagacga agagaggctg gtgcaagttc aagaatatcg aaacgttgtc
     2821 ccatcccatg ttccagagca atgaaaccac ctggtttcaa cacgcggcac gcgtcgccca
     2881 acagtcgatt gatgatctcg agtccgcgct ttcccccatc caacgccaga gatggctcgt
     2941 agtcacgaac ttcgggcatg agtccctgaa gctctgtgga atcgatgtat ggtggattgg
     3001 aaacgatcag gtgcactgaa cggtcgcgac agggagaggt gagatctgcc tgacacaggt
     3061 gaatccgctc ggcttccgcc ggatgtcgct cgaggttgcg ctgagccaca ccaagtgcct
     3121 ggcgagacac atcgatgagc agaccgcgtg cctcgcgaaa ccgatgggcg atcgacaggc
     3181 caatggcccc ggttccggtc ccgacatcga cgaactgaaa actgcttggc cagtccgtgc
     3241 cgggaaggaa gtcaaacagc tcgagaagcc agtcttcggt ctcagggcgc ggaatgagtg
     3301 cgccgggcgc gacccggaga tggaggtcga gaaaccccca ggtgccgagg agatactgca
     3361 atggttcacg ctgctctcga cgtcgagcca gggtttccag ctgatcatgc tgttcagaag
     3421 tcagctgatc gcgcagatgt cgaatcagac ttcccggtga tgctgccaga accagttcga
     3481 gaagactgtc cgccgagatc tcggcatcag gaatgccggc cgacgcaaat cgtgtgcgca
     3541 gggtcaggcg agcgtcgcga accgacacca gaagtcctag gcgatttgtt cggcagcaag
     3601 cttgcgaacg ttgtctgcct cgattaatgc gtgaatgatc tcgtccaatt cgccttccat
     3661 cacggcagag agacgataga tggtgagatt gatgcgatgg tcagtcagcc gctgttgtgg
     3721 gaagttgtag gtgcggatcc gttcagaacg gtctccggaa ccgatctgcg atcgacggtt
     3781 gtccgcccgc tccttgtcgg ctttcgcgtt ggcgagatcg agcaggcgag ctcggagaat
     3841 gcgcaaggct ttggccttgt tcttatgctg gcttttctca tcctgacagg cgaccaccag
     3901 accggtcgga acatgtgtca cccgaaccgc ggagtcagtc gtgttcacgc tttggccgcc
     3961 cgggccggag ctgcgataga catcgatgcg cagatccttt tcatcgatct gaatgtcgac
     4021 ttcttctgct tcgggcaaaa cagcaaccgt tgccgcagat gtgtgaacgc gacctgaggc
     4081 ttcggtttct ggtactctct gcacacgatg gacgccactc tcatacttca accggccgaa
     4141 gacatcctgt ccatctaccg aaaagatgac ttccttgact ccgccgatgc cggtgtcgtt
     4201 ccggctcatg acttcccatt tccagcggcg accgtcggca tacttcgtat acatgcgaaa
     4261 ca
//
LOCUS       DAORHD010000262         4260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22310_length_4260_cov_3.404994, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000262 DAORHD010000000
VERSION     DAORHD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22310_length_4260_cov_3.404994"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..690)
                     /locus_tag="PKO06_06840"
     CDS             complement(193..690)
                     /locus_tag="PKO06_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV69398.1"
                     /translation="MATEKETSKTGSLAGKYLTFQLANEEYGITIMKIREILGIMETT
                     MVPRMPDYVRGVINLRGKVLPVIDLRRKFGLPGVEDTKHTCIIVIDTMQTGIAALVGI
                     IVDTVSEVVQIADADIDRGVTLGSQVDTSFILGMAKVKGDVRILLDIDRILSFAELQH
                     LSGAS"
     gene            complement(730..3039)
                     /locus_tag="PKO06_06845"
     CDS             complement(730..3039)
                     /locus_tag="PKO06_06845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNV69399.1"
                     /translation="MNLTERNDEWRMLVEVFATESMEMLDEVEPRLVELEKNPDPAVI
                     NNIFRLFHSLKGGAASLGLQIIRDLTHEAETLLDLFRSKGKKIESAHIDLMCQSCDLT
                     RQIITKLQEEFDDAEFEAEASALRLELVQAVMDLVDDSPPNLEVYPGAHRNYSPSDRA
                     ASEAVTKPSSVSAAPSKPANPLPGPNLASSIDELKLDITPEMCVGFAAEADELLEAIE
                     TNLVAFEKEPGRTGLLAEAFRAFHSLKGNCGFFGFVDLEHLSHESENLLGAMRDSQIP
                     ADRANVSLVLSTLDVCRATVKSIKASGPATIADLEPMVTRLKQALKTTEAPKLGEILI
                     AKGEVSADALQEALEEQEQQKKTKPAATTPVKSEDSAAKDGAGSGKQSIRVDTEKLDR
                     LIELVGELVISEANVANCAELRNLKIDRIEKSIDQLNKITKDLQNIAMSVRMIPIAGL
                     FRKMVRPVRDLARTFGKKIDLQFIGEETEVDKNVIELVSDPLMHLIRNSVDHGIEPPA
                     DRTKCGKPEEGVIVLEARHAGGEVWIMVRDDGRGLNRDKILAKARQVGLCTTDGSDLR
                     DEEIYKFIFEPGFSTADKVTEVSGRGVGMDVVKRNIEKLKGRVDIKSSPGKGSSMIIR
                     IPLTLAIIEGMVVQVGAERYIMPTQAILEFIRPTSDLLASVIDQGEVINIRGHLIPLF
                     RIASIFGIRNAITDPTQANVMIIEHEGKSYGLMVDQILGQQQTVVKSLGATLGYMQGI
                     AGAAVMPDGKVGLILDLGGLIPLFLGTQM"
     gene            complement(3036..3488)
                     /locus_tag="PKO06_06850"
     CDS             complement(3036..3488)
                     /locus_tag="PKO06_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69400.1"
                     /translation="MNILLIDPHPESLKECCENLSILGYSTEAVQTATDAVSAAQRLQ
                     PGLVVLSCLPPLEAPALLAELQALLPSALFIPWAEKPKVALVLQLLNTHHIHAFSQER
                     PDMRTIMMWAAEAEKKGINLQEREAQHSRLALEYAKLKQAYDDLRGRV"
     gene            complement(3614..>4260)
                     /locus_tag="PKO06_06855"
     CDS             complement(3614..>4260)
                     /locus_tag="PKO06_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69401.1"
                     /translation="IPVNLLYTGRKLMLSASIPFQDWDVRAAGGGPTVTLNGLHDPEI
                     KAGYQIWKNYEGTHAVALHVGGRFPGDNYHQPLVTMTGKSRVGVRVGPANSTRGAWTE
                     FGGAYTGQLSDRWTSHLNLALANDSEDSIARYIYRGMLDYRVNHHLSLMAELNGQTWE
                     MDNGPDGPNVDVTLGCAVFNADWQGYLGFPIAVSHDWGYGHDFGVTLGLNTRWD"
BASE COUNT          830 a         1201 c         1260 g          969 t
ORIGIN      
        1 aaaccccaga tagtcctcga agctgtgcag atgcagggcg cgcaaacgct ttgacacccg
       61 cgcggcgacg agggcttctt ttccttcctt cagggtgatg ccgcttttgt cgtagacgag
      121 acggcagaac gcttgaaagg tttctggttt catgtcgcga tttcgtacct gttacgagaa
      181 cgtgaacgag tctcaggacg ctccggagag gtgttgtaat tctgcgaatg aaagaatgcg
      241 gtcgatgtcg agcaagatgc ggacatcgcc tttcactttg gccatgccca gaatgaaact
      301 cgtgtcgacc tgcgatccaa gtgtgacgcc ccggtcgata tcggcatcgg cgatctgtac
      361 cacttcgctg accgtatcga cgatgatgcc gacgagagcc gcaatgccgg tctgcatggt
      421 atcgatgacg atgatgcagg tgtgcttggt gtcttcgacg cccggcagcc cgaattttcg
      481 gcgcaggtcg atgacgggca gcactttgcc gcgcagattg atgaccccgc gcacgtagtc
      541 aggcatacgc ggaaccatgg tggtttccat gatgccgaga atctcgcgga tcttcatgat
      601 cgtgatccca tattcttcat tggccagctg gaacgtcaga tacttgcctg ccagactgcc
      661 tgtcttgctg gtttccttct cggttgccat agctctcact ccccttctgc ttgaggtgga
      721 cgcccctgat tacatctggg tgcccaggaa cagtggaatc agaccgccaa ggtcgaggat
      781 cagaccgact ttgccatccg gcatgaccgc ggcaccggca attccctgca tgtagccgag
      841 tgttgcgccg aggctcttga cgacggtttg ttgctggccg agaatctgat cgaccatcaa
      901 tccgtatgat ttcccttcgt gctcaataat catcacattc gcctgtgtcg ggtcagtgat
      961 cgcgttgcga atgccgaaga tcgaggcgat ccggaacagc ggaatcagat gaccgcggat
     1021 gttgatcact tcgccctgat cgatgaccga ggccagtaaa tcagaggtgg ggcgaatgaa
     1081 ctcaagaatg gcctgggtgg gcatgatata gcgttccgcg ccgacttgta cgaccatgcc
     1141 ttcgatgatc gccagagtga gcggaatgcg gataatcatc gatgaccctt tgccagggct
     1201 cgacttgata tcgacgcgac ccttgagttt ctcgatattg cgcttgacga catccatgcc
     1261 gacaccccgc cccgagacct cggtgacctt gtcggctgtc gaaaagcctg gttcgaagat
     1321 gaacttatag atctcttcgt cgcgtaagtc cgagccatcg gtcgtacaca ggccgacctg
     1381 acgagctttg gcgaggatct tgtcgcgatt gaggccacgg ccatcatcac gaaccatgat
     1441 ccagacctcg cctccggcgt gacgggcttc gagcacgatc acgccctctt cgggtttgcc
     1501 gcacttcgtg cggtcagccg gcggttcgat gccatggtcg accgagttgc gaatcagatg
     1561 catcaacgga tcgctcacca gttcgatgac attcttgtcg acttcggttt cttcgccgat
     1621 gaactgcaga tcgattttct tgccgaaggt tcgcgccaga tcgcgtaccg ggcgaaccat
     1681 tttgcgaaac agcccggcaa tcggaatcat gcgcacggac atggcgatgt tctgcagatc
     1741 tttggtgatc ttgttcagct ggtcgatcga cttttcgatg cggtcgatct tcagattacg
     1801 cagttcggca cagttggcaa cattcgcctc cgagatgacc aattcgccga ccagctcgat
     1861 caggcgatcg agtttttcgg tatcgacgcg aatgctctgt ttgccgctgc cggcaccatc
     1921 tttggcagcg ctatcctcgg atttgaccgg tgtcgtggcg gcaggcttgg ttttcttctg
     1981 ctgctcctgt tcttcgagag cctcctgcag ggcatcagcc gacacctccc ctttggcgat
     2041 cagaatttcg cccagcttgg gggcctcggt tgttttcaag gcttgcttca gacgggtgac
     2101 catcggttcc agatcggcaa tggttgcggg gccgctggct ttgatgcttt tcaccgtggc
     2161 ccggcacacg tcgagcgtgg aaaggaccag gctgacattg gcacgatctg ccgggatctg
     2221 gctgtcacgc atggcaccaa gcaggttttc cgactcgtga ctcagatgtt cgaggtcgac
     2281 gaacccgaag aagccgcagt tgcctttcag actgtgaaaa gcgcgaaagg cctcggccag
     2341 cagcccggtt cgaccgggct ctttctcgaa ggcgacgaga ttggtctcga tggcctcgag
     2401 cagttcatca gcttcggcag cgaaccccac gcacatctcg ggggtgatgt cgagtttgag
     2461 ttcatcaatg gaacttgcca ggttcggccc tggtagcggg ttggcgggtt ttgagggcgc
     2521 ggccgagacc gaactcggct tggtgacagc ctcagaggcg gcacggtctg acggggaata
     2581 gttgcggtgc gcacccggat acacctcgag gttgggcgga ctgtcatcga cgagatccat
     2641 gaccgcctgc accagttcga gtcgcaacgc tgaagcttcg gcctcgaact cggcgtcgtc
     2701 aaattcttcc tggagtttgg tgatgatctg gcgcgtcaga tcacagcttt ggcacatcag
     2761 atcgatatgg gccgactcga tcttcttgcc tttgctgcgg aacaggtcga gcagcgtttc
     2821 cgcctcgtgc gtgagatcgc gaatgatctg cagaccgagc gatgccgctc cgcctttcag
     2881 cgaatggaaa aggcgaaaga tgttgttgat gaccgccggg tcggggttct tctcgagctc
     2941 gaccagtctc ggctccacct catcgagcat ttccatgctc tctgtggcga agacctcgac
     3001 cagcattcgc cattcatcgt tgcgttccgt gaggttcaca cgcggcctcg cagatcatcg
     3061 tatgcctgct tgagtttggc atattcgagg gcgagacgcg agtgctgtgc ttctcgttcc
     3121 tgcagattga tgcccttctt ctcggcttca gccgcccaca tcatgatggt ccgcatgtcc
     3181 gggcgttcct gcgagaaggc gtgaatgtgg tgggtgttca gaagctgcag taccagcgcc
     3241 actttgggtt tctcggccca gggaatgaac agcgcgctgg gtagcaaagc ctgaagctca
     3301 gcgagcagcg ccggcgcttc gagaggcggc agacaggaaa gcaccaccag gcctggttgc
     3361 agccgttgtg ccgcggaaac ggcatcggtc gctgtctgca cagcttcggt ggaatacccg
     3421 agaatggaaa gattttcgca gcactctttc aggctctcgg gatgcggatc gatcaggagt
     3481 atgttcatag gctctcgtac ctctctcggt agcagtattt cacgttccca tccgcttgtc
     3541 aacgaaacca aaccgcacgt aaaaacggcc cccgggagtt ccgggggccg tagtgtggct
     3601 ggttgaggaa aatctagtcc cagcgggtgt tgagacctaa ggtgacaccg aagtcgtgcc
     3661 cgtagcccca atcgtgagag accgcgatcg ggaacccgag atagccctgc caatcggcgt
     3721 tgaagacggc gcaacccagc gtcacatcaa cgttcgggcc gtcaggtccg ttgtccattt
     3781 cccacgtttg accattcagt tccgccatca gtgacaggtg atggttcacg cggtagtcga
     3841 gcatgccccg gtagatgtag cgggcaatgc tgtcttcaga gtcattggcg agagccagat
     3901 tcaggtggct ggtccagcga tcgctcagct ggccggtgta agcaccaccg aactcggtcc
     3961 aggcaccacg ggtcgaattg gcggggccaa cgcgcactcc gacgcgactc ttgccggtca
     4021 tcgtaaccag gggctgatga tagttgtcgc ctgggaacct gcctccgaca tgcagagcca
     4081 cggcatgggt gccttcgtaa ttcttccaga tctggtaacc ggccttgatt tcaggatcat
     4141 gcaggccatt gagagtcacg gtagggccac cgccggcggc gcgaacatcc caatcctgga
     4201 acgggatgga ggcgctgagc atcagtttgc ggcccgtgta gagcaggttc acggggatca
//
LOCUS       DAORHD010000263         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22371_length_4253_cov_3.345641, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000263 DAORHD010000000
VERSION     DAORHD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22371_length_4253_cov_3.345641"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKO06_06860"
     CDS             <1..468
                     /locus_tag="PKO06_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNV69402.1"
                     /translation="TVEGDLGVYVNAKQVLENIEDPEFAARIADLRAMPQSEREKEFT
                     RRLCAKAVEIGARRHAGTLSDLYTPTGRKQIVRGKDLTAVRWVIGTGGALTRIEGGIE
                     ILRSICRGPEKHLLPRPDATVLLDRNYLFSALGTLAQAYPAEAGRTLTRFVTT"
     gene            complement(660..3008)
                     /locus_tag="PKO06_06865"
     CDS             complement(660..3008)
                     /locus_tag="PKO06_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69403.1"
                     /translation="MPDIKFLPIHQYDTSTLPLAMQNALDAAVAVCKRLGHTCIGLPH
                     LMLGILRNCPSLVATKVSEIDREQVEKSLLKSLEEHGQEFNQDLTHVSMPETLDRIIR
                     RVFRASGSDGGKADFLTEVESQLPGQVDHWLKLSEPVTAPDAANPQRSAPSTASTPQP
                     IRTGINLVPMPQKSEPPKPFPGPRPGSEAPQVPAEKGETKPTPVVRPPYTRLWEAEEI
                     ATFPAEAPGRDWELQALALELSRRGASGIVVGGSTKCGKTCLMQVFARKATAGTWKNL
                     ADTRFFQLDVPALMTNVLAGKVSHADFAETLNKLAQIQNAVLIVDPAHVFFGLGGPPG
                     YHDLYAVMRGILLQKSLRLVFVTTPEFLKSVSVDLVFSALPQIQLGLLESPDIVRTVE
                     QSCSLLEKTFNVIIPKAVVPRVVSLHEENGRKRILPYQAIRTLESACIVAVQNGDSKI
                     TVEHVAAAFARENRSIDAASPERLGHIEEELGKWVKGQIEAIRAVAPRIRMTRLQLDR
                     NPNRPDGVFLFLGPSGVGKTELAKAITWALYGDLTRLVRVDMSEFMSQHEYSKLIGAP
                     PGYIGHGEEGHLTGPVAKLGHGVILLDEIEKAHPSILKMFLQVFDEGFLTDGQGRRVD
                     FSSFVIVMTSNMGRELYAEGKSRLGFLSEASKDETPDADAALQYLLKVLPSEFINRVD
                     QAVPFKPLAREHLAEIARKLIDEEAARWLKRDRVLTFGEGIIDFLVDHGYDPRLGARH
                     LARNFERTVSQTLSDAFCSQEWGKLQRLRLDITDGKPKLSPV"
     gene            complement(3094..3561)
                     /locus_tag="PKO06_06870"
     CDS             complement(3094..3561)
                     /locus_tag="PKO06_06870"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HNV69404.1"
                     /translation="MAQLTPEEIQKGIEATTAKVERYLRKMGTYEVVGGDTYRFRGES
                     TVVTIRVQTFSSGAPFVRVLAPVLREVKKAGNEAMFEEFSKLNSDSVIGKIYWEENRD
                     TPGFGFIFAEHNLLGEFLDYEELLAAVQVIIGSADDLDDRLQAKYGGKRWIDS"
     gene            3913..>4253
                     /locus_tag="PKO06_06875"
     CDS             3913..>4253
                     /locus_tag="PKO06_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69405.1"
                     /translation="MFDRRLNLRYLLQKKSFFLFGPRSTGKTTLVQRSLPDALVYDLL
                     DAEVYQQLLRRPKILEEVAAVERRLIVIDEIQKLPQILDEVHRLIQKRDVRFLLTGSS
                     ARVCLRRVAAD"
BASE COUNT          835 a         1279 c         1241 g          898 t
ORIGIN      
        1 accgtcgaag gcgatctcgg ggtgtacgtc aacgcgaaac aggtgctcga gaacatcgag
       61 gaccctgagt ttgccgcgcg catcgctgat ctgcgggcca tgccgcagag tgaacgcgag
      121 aaggagttca cccgccgact ctgtgccaag gccgtcgaga tcggcgcccg tcgccacgcc
      181 ggcacgctat ctgacttgta cactccaacc ggccgcaaac agattgtccg aggcaaagac
      241 ctcaccgcgg tgcgctgggt catcggtacc ggcggagcac tcacgcggat cgagggcggc
      301 atcgagattc tgcgctcgat ctgccgcggc cctgaaaagc acctgctgcc gcgccccgac
      361 gcgaccgtcc tgctcgaccg caactacctc ttttccgccc tcggcaccct ggctcaagcc
      421 taccccgccg aagccggccg caccctcacc cgcttcgtca ccacctgagc cggttggagc
      481 cgcgtgcgtc caaaaaatcc cgctgaacga attgacgacg gcctgtcacc ccgacgatcc
      541 ggttcatctg gttcttccat cagtacagca tcatgaggtc caggggcctc tggtccctgg
      601 cggggtgccg gggcagagcc ccggtctttg gtttctgctt cttctgttcg ctctccgtgt
      661 cagaccggtg acagcttggg cttgccgtca gtaatgtcga gacgcaggcg ctgcagtttc
      721 ccccattcct ggctgcagaa agcgtctgag agggtctgag atacggtgcg ctcgaagttg
      781 cgtgccaggt gacgggcccc gagacgcgga tcgtagccgt ggtcgaccag aaagtcgatg
      841 attccctcgc cgaacgtcag tacccgatcg cgtttgagcc agcgggcggc ttcctcgtcg
      901 atgagcttgc gggcaatctc ggccagatgc tcgcgtgcca gcggcttgaa cggcaccgcc
      961 tgatcgacgc ggttgatgaa ctcgctgggt agcaccttca agagatactg caacgcggca
     1021 tctgcatccg gagtttcatc tttggaggcc tctgacagaa atccgagcct ggactttcct
     1081 tcggcataca actcgcgccc catgttcgat gtcatgacga tgacgaacga actgaagtcg
     1141 acgcgacgac cctggccgtc ggtaagaaag ccttcgtcga aaacctgcag aaacatcttc
     1201 agaatcgagg ggtgcgcctt ttcgatctca tcgagcagaa tcaccccgtg acccagtttc
     1261 gcgacggggc cggtcaagtg accttcctcg ccgtgtccga tgtagccggg gggagcgccg
     1321 atcagctttg aatactcgtg ctggctcatg aactcggaca tgtcgacccg caccagacgg
     1381 gtgagatcgc catacaacgc ccaggtgatc gctttcgcca gttccgtctt gcccacgccc
     1441 gaaggcccga gaaacaggaa gacgccgtcg gggcggttgg ggttgcgatc gagctgcagt
     1501 ctggtcatac gaatgcgcgg ggcgacggcg cgaatggctt cgatctgccc cttgacccac
     1561 ttgccgagct cttcttcgat gtgtccgaga cgctcgggtg aggcggcgtc gatcgagcgg
     1621 ttctcgcgcg cgaatgccgc ggcgacgtgc tcgacggtga tcttactgtc tccgttctgt
     1681 accgccacga tacacgccga ttcgagcgtg cgtatggcct gatacggcag aatgcgcttg
     1741 cgaccgtttt cctcatgcag gctcacgact cgcggcacga cggctttggg aatgatgacg
     1801 ttgaaagtct tttccagaag cgagcagctc tgttcaacgg ttcgtacaat gtcggggctt
     1861 tcgagcagtc cgagttgaat ctgcggcaac gcactgaata ccagatcgac gctcaccgac
     1921 ttcaggaact caggcgtggt gacgaacacc agacgcaacg acttctgcag cagaatgccg
     1981 cgcatcaccg cgtagagatc gtgatagcct ggtggcccgc ccagcccgaa gaacacgtgt
     2041 gccgggtcaa cgatcaatac cgcattctga atctgtgcca gtttgttcag agtctcggcg
     2101 aaatccgcat gactgacttt tccggccagc acgttggtca tcagcgccgg aacatctaac
     2161 tggaagaacc gcgtgtcggc aagattcttc caggtgccgg ccgttgcttt acgggcgaag
     2221 acctgcatca ggcaagtttt gccgcatttg gtcgagccgc cgaccacgat gcccgatgcg
     2281 ccgcggcgcg acagttcgag cgcaagcgcc tgcagttccc agtctcgccc gggtgcctct
     2341 gccggaaagg tggcgatctc ctcggcttcc cagaggcggg tatacggggg gcgaacgacg
     2401 ggtgtcggtt tggtttcccc tttttccgcg ggaacctgag gtgcttctga tcctggtctg
     2461 gggcctggaa aaggtttcgg cggctcggac ttttgcggca tgggcaccag atttataccg
     2521 gtgcgaatcg gttggggcgt gctcgccgtc gacggagcgc tgcgctgagg attcgcggcg
     2581 tccggcgcgg tcaccggttc cgagagtttc agccagtggt cgacctgacc tggcaactgt
     2641 gactcgacct cggtcagaaa gtccgctttg ccgccatccg aacctgaggc gcgaaagacc
     2701 cggcgaatga tgcggtcgag cgtttccggc atcgatacgt gggtgagatc ctgattgaac
     2761 tcctgcccat gctcttcgag cgacttgagc aatgatttct cgacttgctc acgatcaatc
     2821 tcagatactt tcgtcgccac cagactggga caattccgca gaatccccag catcagatgc
     2881 ggcaggccga tgcaggtgtg tccgagccgc ttgcagacgg caacggctgc atcgagagcg
     2941 ttctgcatag ccagtggcag ggttgaggta tcgtattgat gaatgggcag aaacttgata
     3001 tcaggcatga gaaacccttt cccaaccgga actcaacgtt ctccggtccg gtgatgaatc
     3061 agagagtggt ggcttcgaat agcggctgtg ctgtcagctg tcgatccagc gcttgccgcc
     3121 gtatttggcc tgcagtcgat cgtccaggtc gtcggcgctg ccgatgatga cctgaaccgc
     3181 agccagcagt tcctcgtaat cgagaaactc cccgagcaga ttgtgttccg cgaaaatgaa
     3241 gccgaaaccg ggtgtgtcgc gattctcttc ccagtaaatc ttcccgatga ccgagtctga
     3301 gttgagtttc gagaactctt caaacatcgc ttcgttgcct gctttcttga cctcgcgcag
     3361 caccggtgcc agcacgcgca cgaacggcgc gccgctcgaa aaggtttgca cccgaatcgt
     3421 caccaccgtc gactcgccgc gaaaccgata cgtatcgcct ccgacgacct cgtacgttcc
     3481 catcttcctg agataccgct cgaccttcgc cgtggtcgct tcaatcccct tctgaatttc
     3541 ctcgggcgtc aactgagcca tgtcgcatcc tcctcctgaa atgaactgcc gccaaaatca
     3601 tacccttaac ccgcctgttt ctcaaccaga atagcgatcc cagcaggggg cgttttgccg
     3661 gcagatggcg ctcggccgaa gcgaaagcat tctctggaag aaccgcatcc gggtcagcag
     3721 cgacccggtt aattacaggc actctctagg catttcggtt gtgatgcgta gaagagagag
     3781 tccgcgcggg cctcgcgagg aaatcgaaaa gaggaggctg gggatgaatc gttgcttgcc
     3841 tgtgatcctg aaaaaacgta tagtatatgt ggattatgat gataaaccgc aatattgttg
     3901 gggaaaattg acatgttcga tcgacgacta aatctgcgct accttctgca gaagaagtct
     3961 ttcttcctgt tcggtccacg atcgacgggc aagacgacgc tggttcagcg gtctcttccg
     4021 gatgctctcg tgtacgatct gcttgatgcc gaggtctatc aacagcttct gcgtcgcccc
     4081 aaaattctcg aggaagtggc ggcggtcgaa cggcgtctca tcgttatcga tgagattcag
     4141 aaactcccgc agattctcga cgaagttcat cgtttgatcc agaaacgcga tgtgcgattc
     4201 ctgctcacgg gaagctcggc ccgcgtctgc ctgcggcgcg tcgccgcgga ctt
//
LOCUS       DAORHD010000264         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22387_length_4251_cov_3.655863, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000264 DAORHD010000000
VERSION     DAORHD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22387_length_4251_cov_3.655863"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKO06_06880"
     CDS             complement(<1..262)
                     /locus_tag="PKO06_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69406.1"
                     /translation="MNIALIRRFLASSLVLMFLLCTVAPMWLFAQAGSGDAEILPKKT
                     ESESTSPSGTSTTTPAPTTATPASTDDLSPFSLSVIILSMLIG"
     gene            complement(282..1655)
                     /locus_tag="PKO06_06885"
     CDS             complement(282..1655)
                     /locus_tag="PKO06_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV69407.1"
                     /translation="MRHPVRPLLYRTGCFFLFLWVLLTTAPLVGQSTEPATANLTRGK
                     VIVIPIHGDIESGVSFFVQRMLRRADQEQAAALVLEINSNGGLVSAAQEIKTALLESR
                     VKTIAYVKGRALSAAALIAISCQKILMEPGAEMGAATPIQLMGDGVRAAEEKFVSAFR
                     AEFESAAEKNRRPTNLAGAMVDKNHDEIPGLVKRGEILTLTTEKAATHGYCDFIVSTL
                     ESGLRQMKIEPTPLERVEPTSGEWIARWLTNPNISVLLFSAGVWLIILEFAVFGWGLL
                     GWLGLLLLALFFGGHLFAYLAGLEAVLLFVFGMVLLLIEFFIIPGFGLTGIAGILSLC
                     MSIIMVFGGIYSAIYAIGKIMAISILMIVALYHFAPRLKLFDGVVLKQELTTEAGFVA
                     VDNTQFDHLLNLEGITQCPCRPAGIARIGTERVDVVSEGDFIERNQRVVVVRVEGQKI
                     VVRELKA"
     gene            complement(1678..1866)
                     /gene="rpsU"
                     /locus_tag="PKO06_06890"
     CDS             complement(1678..1866)
                     /gene="rpsU"
                     /locus_tag="PKO06_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNV69408.1"
                     /translation="MAEVRVMKEEPIEKALKRFQKKCQEAGIIKEIKRRRAYEKPSEK
                     AKRKTAEARRKQRRRGGR"
     gene            complement(1913..2257)
                     /locus_tag="PKO06_06895"
     CDS             complement(1913..2257)
                     /locus_tag="PKO06_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNV69409.1"
                     /translation="MNETCLFCKIIAGKIPSDTVYRDEHVVAFRDIQPQAPVHVLVVP
                     TTHVAHLQEPDAVSGKILEPVFRTIQKLADQFELDKGFRVVVNCGEEGGQSVAHLHFH
                     LLGRRRMAWPPG"
     gene            2496..2568
                     /locus_tag="PKO06_06900"
     tRNA            2496..2568
                     /locus_tag="PKO06_06900"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2529..2531,aa:Pro,seq:tgg)
     gene            2592..2664
                     /locus_tag="PKO06_06905"
     tRNA            2592..2664
                     /locus_tag="PKO06_06905"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2625..2627,aa:Gly,seq:gcc)
     gene            2685..2758
                     /locus_tag="PKO06_06910"
     tRNA            2685..2758
                     /locus_tag="PKO06_06910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2719..2721,aa:Arg,seq:tct)
     gene            2783..2854
                     /locus_tag="PKO06_06915"
     tRNA            2783..2854
                     /locus_tag="PKO06_06915"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2815..2817,aa:His,seq:gtg)
     gene            2883..2956
                     /locus_tag="PKO06_06920"
     tRNA            2883..2956
                     /locus_tag="PKO06_06920"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2917..2919,aa:Arg,seq:tcg)
     gene            2960..3031
                     /locus_tag="PKO06_06925"
     tRNA            2960..3031
                     /locus_tag="PKO06_06925"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2992..2994,aa:Lys,seq:ttt)
     gene            3060..3132
                     /locus_tag="PKO06_06930"
     tRNA            3060..3132
                     /locus_tag="PKO06_06930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3093..3095,aa:Lys,seq:ctt)
     gene            3244..>4251
                     /locus_tag="PKO06_06935"
     CDS             3244..>4251
                     /locus_tag="PKO06_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69410.1"
                     /translation="MRANLSLRLLLLLLLLVVATSTLVEASAKLRLDVGLNHFYKKRY
                     LEAFREFKAAAEEDPRNADAHYNLGRVYKIQGFLKEAVAEFQVALALNPNLTAARREL
                     AEIQGFIKSDVTTQLKLQGQEEALKQRITDVGSNSALKRGQDLLNRGDIARAIPEFEQ
                     AVQADPYNAKILKVLGYLYFRSNRYANALSSYERAQRLAPNDAEIAYDLGLIHLRSDN
                     ARSAADSFSRAIALDSGLVKAHYGLGEAYESLGQFEDAAFQYRKCLELNPGLRQAEDR
                     VRELSGKLGYMYFTRGSFYYQQGDFEKAEALLSLSAKYGSLTDAQKRQVDDMLGAARY
                     WV"
BASE COUNT          888 a         1188 c         1245 g          930 t
ORIGIN      
        1 cgccgatcaa catgctgaga atgatcacgc tcagagaaaa cggcgacaga tcatcggtgg
       61 aggctggcgt cgctgtcgtc ggcgcggggg ttgtggtcga cgtccccgat gggctggtcg
      121 attcggactc ggtcttcttc ggaagaattt ctgcgtcacc tgacccggcc tgggcgaaca
      181 gccacattgg cgccacggta cagagcagga acatcagtac cagtgagctg gcgagaaacc
      241 gccgtatcaa agcgatgttc atgggattcc ctttcgaacg ttcaggcttt caattctcgc
      301 accacgatct tctggccttc gacacgtacc acgaccacac gttgattgcg ttcgatgaag
      361 tcgccttccg agacgacatc gacgcgctca gtaccgatgc gggcgatgcc ggctggccga
      421 cacgggcact gcgtgatgcc ctcgaggttg agcaggtggt cgaactgggt gttgtcgacg
      481 gcgacgaagc cggcctctgt cgtcagttcc tgcttcagaa cgacgccatc gaacagcttc
      541 aggcgcgggg caaaatggta caaggcgacg atcatgagaa tcgagatcgc catgatcttg
      601 ccgatggcat agatagcgga atagatgccg ccgaacacca tgatgatgct catgcacagc
      661 gagagaatcc cggcgattcc cgtcaggccg aatccgggga tgataaaaaa ctcgatgagc
      721 aggagcacca tgccgaagac gaacagcaga accgcttcga gaccggcgag gtaggcgaac
      781 aggtgcccgc cgaagaacag ggccagcagc agcaggccca gccagcccag caggccccag
      841 ccgaagacgg cgaactcaag aatgatcagc cagacgccgg cggaaaacag caggacggaa
      901 atgttggggt tggtcagcca gcgggcgatc cattccccgg aggttggctc gacgcgttca
      961 agaggcgtgg gctcgatttt catttgccgc agcccgcttt cgagtgtcga cacgatgaaa
     1021 tcgcagtagc cgtgggtggc ggctttttca gtagtcaggg tgaggatctc gccccgtttg
     1081 acgagccccg gaatctcgtc gtgattcttg tcgaccatgg ctccggccag gttggtcggt
     1141 cgacgattct tctcggcggc tgattcaaac tcagcccgaa aagccgagac gaacttctct
     1201 tcggcagccc gcacgccgtc gcccatgagt tgaattggcg tcgcggcacc catctcggca
     1261 ccgggttcca tgagaatttt ctgacaggaa atcgcgatca gagccgcagc tgaaagcgcg
     1321 cgccctttga cgtaggcgat ggtcttcact cgactttcaa gcagggcggt tttgatctcc
     1381 tgggcggcac tgaccaaccc gccgttggag ttgatttcga gaaccagggc ggctgcctgc
     1441 tcctgatcgg cacggcgcaa catgcgctgc acgaagaagg agacgcccga ttcaatatca
     1501 ccgtgaatcg gaatgacaat gaccttgcca cgggtgagat tggccgtggc aggctcggtc
     1561 gattgcccga ccaatggtgc ggtcgtcaga agcacccaca ggaaaaggaa aaaacacccc
     1621 gtcctgtaca acaacggtcg gacggggtgt ctcatggttc gaacctcggt gggggactta
     1681 gcggccacca cggcggcgct gcttgcggcg agcctcagcg gtcttgcgct tggctttctc
     1741 gctgggtttt tcataagccc ggcggcgctt gatttccttg atgataccag cctcctggca
     1801 cttcttctgg aagcgcttca gagctttctc gatcggttct tctttcataa cgcgaacttc
     1861 tgccatacta tcacccccct cccggcatgg tcttcacaga gcaatggatt gttcagcccg
     1921 gaggccaagc cattctccgg cggcccagaa ggtgaaagtg cagatgtgct accgactgtc
     1981 cgccttcttc tccgcagttg acgaccactc gaaagccctt gtcgagctcg aactggtcgg
     2041 ccagtttctg aatcgtacga aacacgggtt ccaggatctt gccgctgacg gcatcgggct
     2101 cctgcaggtg cgccacatgc gtggtcggca ccaccagaac atggaccgga gcctgaggct
     2161 ggatatcacg gaacgccacc acgtgttcgt cgcggtacac cgtgtccgat gggatcttcc
     2221 cggcaatgat tttacagaac aggcatgttt cgttcatgtg tgaagcacct atattagaga
     2281 gattttctgc ctatgtcaag tttttccgcc ggcggatacg ctgatgaata gtcatatcgt
     2341 ttcgggcgat tgtcgagtat tgggcttgca acatcgtttc tgttgctggt tctgccagct
     2401 tgtacacctg agccgcgtac atttttcgaa aagatgcttg acttttgttt aggcctctgg
     2461 taaccttgct cttacgttgc gacggtaacc atccgcgggg tgtggctcag ttggtagagc
     2521 gcttggtttg ggaccaagat gtcgctggtt cgagtccagt caccccgact ggcaacgacg
     2581 tgatgtggta tgcgggaata gctcagttgg tagagcgcca ccttgccaag gtggatgtcg
     2641 cgggttcaag tcccgtttcc cgctttccgg tcccctccga tgttgcgcct gtagctcagg
     2701 tggatagagc aacagccttc taagctgtgg gccagaggtt cgagtcctct caggcgcgtc
     2761 cgctgcgatc ggcagggtta tggagaaagt agctcaatgg cggagcacaa gattgtggat
     2821 cttgtggttg cgggttcgag tcccgtcttt ctccctgtcc gttcgttcta gactgcagag
     2881 ccgcgcctgt agctcaattg gatagagcaa cggacttcgg atccgtaggc tgagggttcg
     2941 aatccttcca ggcgcatgcg ggccgctagc tcagtggtag agcacctgcc ttttaagcag
     3001 ggagtcgttg gttcaattcc aacgcggctc agtgggatag agaagtgtca gagcagtatg
     3061 gaccattagc tcaatcggta gagcaactga ctcttaatca gtaggttgaa ggttcgagtc
     3121 cttcatggtc cactggaaaa ccccggggtt ccaaaacaga accccggggt tttcgtctgt
     3181 ccggcggata tgctattctg ccgatagata aaaacaggac cctctgaata ctgaggtgta
     3241 tttatgcgcg cgaatctgtc tctgcgtttg cttctgctcc tgctcctgct ggtcgtggcc
     3301 acgtcgaccc tggtcgaggc gtcagcaaag ctgcgcctcg atgtcggtct caaccatttc
     3361 tacaaaaagc gctacctcga agcgtttcgt gagttcaagg ccgccgccga agaagacccg
     3421 cggaatgccg atgcccacta taacctgggc cgggtctaca agatccaggg ctttctcaag
     3481 gaagccgtcg ccgagtttca ggtagcgctt gcactcaatc cgaatctcac cgcggcgcgt
     3541 cgggaactgg ccgagattca aggcttcatc aagagtgacg tgaccacgca gctcaaactg
     3601 cagggccagg aagaagcgct caagcagcga atcaccgatg tcggcagcaa ttccgctctg
     3661 aagcgcggcc aggatctgct gaaccgcggc gatatcgcgc gcgcgattcc ggaattcgaa
     3721 caggccgtgc aggctgatcc gtacaacgcc aaaattctca aggtgctcgg gtatctgtac
     3781 tttcgctcga atcggtatgc caacgctctg agctcgtatg aacgcgcgca acgtctggcg
     3841 ccgaacgatg ccgaaatcgc ttatgatctt gggttgattc acctgcgcag tgacaatgcc
     3901 cggagtgcag ccgactcgtt ttcgcgagcc atcgcactcg atagtggtct cgtcaaagcc
     3961 cattacggtc taggtgaagc ctatgagtca ctcggacaat tcgaagacgc cgcgtttcag
     4021 tatcgcaagt gtctcgaact caaccctggg ctcaggcagg cggaagatcg cgttcgtgag
     4081 ctttcgggca agcttggcta catgtacttt actcgcggtt cattctacta tcagcagggt
     4141 gactttgaga aagccgaagc cctgttatcg ctctccgcca aatacggatc tctgaccgat
     4201 gcccagaagc ggcaggtgga tgacatgctt ggcgcggccc gctactgggt c
//
LOCUS       DAORHD010000265         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22389_length_4251_cov_3.075310, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000265 DAORHD010000000
VERSION     DAORHD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22389_length_4251_cov_3.075310"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKO06_06940"
     CDS             <1..541
                     /locus_tag="PKO06_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014102560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV69411.1"
                     /translation="ETVNCLRNHGASISEEQRHHGPKPYILAEFNLLGYNYRLTDIQA
                     AVGIQQLKKLDRFLDERRKAAGFYASELKSVKWLGLPVVPDGYEHAWQSFVCRVDPKT
                     APCPRNEIMEKLQERGISTRPGTHAVHMLGYYRDRYDIKPDDFPGAKFCDQQTMAIPL
                     HNRMSQEDYEYVVSALKSL"
     gene            591..1397
                     /locus_tag="PKO06_06945"
     CDS             591..1397
                     /locus_tag="PKO06_06945"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69412.1"
                     /translation="MDNKADENKLFWNELCGTSAFRALGLSEVNRESLRTFDNWYFEY
                     YSYLPSYLNLHELSAKRVLEIGLGFGTVGEALFSSSMKYVGVDYAENPVRLMRQRIRW
                     REKQDSACVIQSDARCLPFEDESFDYVVSLGCLHHTGDTEKSIREVHRVLKKPGKAII
                     MLYREGGIKTKLVIPLKYVVRKLCGMKMNYQSYKNACFDSNLDESAAPIIDLLSKRRV
                     KECFARFSHARVAIENLEITSGRIQQILRTTFNSSLGRMFGQDYYITAEK"
     gene            1476..2588
                     /locus_tag="PKO06_06950"
     CDS             1476..2588
                     /locus_tag="PKO06_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV69413.1"
                     /translation="MHSVAVTIVGAGVVGLAIAHALSELGKAQVVLLEKNEKFGQEVS
                     SHNSEVVHCGMYYPTNSLKAKLCVEGRRLLYAFCDRHGVPYRKLGKLIVAIETDEVRR
                     LEAFLQQGLANQVEGLSLLSEGEAKRLEPCVRAKAAIHSVETGIIDSHALMKRLFNLC
                     RDRHVMMAFSSSLSHIERVRGCYEIGVNGDNYRFQSRIVVNASGLGSDEVLKLIGLDP
                     VAKGYKLFLVKGNYFAYHRPSPVSRLIYPLPHQDLKGLGVHATLDLGGRLRFGPDVEN
                     VETIDYDVCVDRKQSFHESAAKMIDGLDPEAFEPDMAGVRPKLAASGFRDFVIQHESD
                     AGFPNLINLVGIESPGLTSALAIADEVKKMVKQLEA"
     gene            2591..>4251
                     /gene="asnB"
                     /locus_tag="PKO06_06955"
     CDS             2591..>4251
                     /gene="asnB"
                     /locus_tag="PKO06_06955"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV69414.1"
                     /translation="MCGIAGIFNLNGEPVSPVVLRKMTDAIAHRGPDGEGFYSDGFIG
                     LGHRRLAIIDLSPAGHQPMLSRDGQVILSFNGEVYNFQELRVELEALGQHFRSRTDSE
                     VVLNAWVEWGADCVRRFNGMFAFAIWDKREQALFLVRDRYGVKPLYYACWGQTFLFGS
                     EQKAILTHPAARRELDKKALLEYFTFQNIFTDRTLLRGVRLLPPASMVRVELGSVRKP
                     DIKRYWDYCFREPEQPADLREYREELDRLIQQAVRRQLVTDVEIGSYLSGGMDSGTLT
                     ALAARELPYIKTFTCGFDLSSASGIELAFDERGKAEAMSARFKTEHYEMVLKAGDMER
                     CLPRLAWHIEEPRVGQSYPNFYAAQLASKFVKVVLSGAGGDELFGGYPWRYYRAAVCD
                     DFEHYIDQYYVFWQRLIPNTVIKKVFAPIWDDVKDVWTRDIFRNVFLTHESKLERPED
                     YINHSLYFEAKTFLHGLLVIEDKLSMAHSLETRVPFLDNDLVDFAMKCPVTLKLNNLA
                     EVVRINENEAANKKGKYFQKTNDGKQILRDVMKKYIPEEVVVADK"
BASE COUNT         1034 a          942 c         1174 g         1101 t
ORIGIN      
        1 ggaaacggtg aattgtctgc gaaatcatgg tgccagcatc tctgaggagc agcgccatca
       61 tggccccaaa ccgtacattc tcgcggaatt caaccttctc gggtacaact atcgattgac
      121 ggacattcaa gcggcggtgg gtattcaaca gttgaaaaaa cttgatcggt tcctggatga
      181 acgacgcaaa gccgctggct tctatgcgtc cgaactgaaa tccgtgaagt ggcttgggct
      241 tcccgtggtt ccagatgggt atgagcatgc ctggcagtca tttgtctgtc gggtcgaccc
      301 gaaaaccgcg ccttgtcccc gtaatgagat catggaaaaa cttcaggagc ggggcatcag
      361 cactcgacct ggtacgcatg cggtgcatat gcttggctac tatcgcgatc gatacgatat
      421 caagcctgac gattttcctg gcgcaaaatt ctgtgatcag cagacgatgg ctattcccct
      481 gcacaaccgc atgagtcagg aagactatga gtatgtcgtc agcgctctga agtctctctg
      541 atgcatgacc gcaagaaagt gtcgactgcg gtttatagat aggacgtagc atggataaca
      601 aagctgacga gaacaaactg ttttggaacg agttgtgcgg tacaagtgct tttcgggctc
      661 ttggtctgtc tgaggttaat agagaatcac tacgcacatt tgataactgg tatttcgaat
      721 actattccta tctgccgtct tacttgaacc ttcacgaatt gtcggcgaag cgtgttcttg
      781 agatcggttt gggttttggt actgttggtg aagcactctt ttcttcttca atgaaatacg
      841 ttggggttga ttacgctgaa aatccagttc gtttgatgag acaaagaatt cggtggcgag
      901 aaaagcaaga ttccgcgtgt gtcattcaga gtgatgcgag gtgtctcccg ttcgaggatg
      961 agtcttttga ctatgttgtt tctcttggct gtttgcacca cacaggcgat accgaaaaat
     1021 caattcgtga agttcatcgt gtattaaaga aacctggaaa agcgataata atgctttaca
     1081 gagaaggtgg aattaaaacg aagcttgtca tcccgttgaa gtatgttgtt cgaaaactct
     1141 gtggaatgaa gatgaattat cagtcatata aaaatgcgtg tttcgactcg aatcttgatg
     1201 aatctgccgc tccaattatt gatttactct cgaaaaggcg tgttaaggaa tgttttgcga
     1261 ggttttctca tgcgagagta gcaattgaga atttggaaat aacctcggga agaattcagc
     1321 agattcttcg gactaccttc aactcaagcc ttggaagaat gtttggacaa gattactaca
     1381 ttacggcaga gaagtgattg gatgagactc agggaccgcg atttccgtgt gtgaaagagc
     1441 gtgacaatct ttttcgttca caaaggagat agggaatgca cagcgtagct gtgacaatcg
     1501 ttggtgccgg ggttgtcggc ttggcgattg cgcatgccct ctctgagctt ggtaaagccc
     1561 aggtagtctt gttggaaaag aacgagaagt ttgggcagga agtcagcagt cacaacagcg
     1621 aggttgtcca ttgcggaatg tactatccga caaactcgct caaagcgaag ctgtgtgtcg
     1681 aagggcggcg gttgctctac gcgttttgcg accggcatgg cgttccttat cgcaaactgg
     1741 ggaagctaat cgtcgcaatt gaaaccgatg aagttcgtcg ccttgaagcc tttctccagc
     1801 agggtttggc gaaccaggtc gagggccttt ctcttctcag cgaaggcgag gcgaaacgac
     1861 ttgaaccttg tgttcgggca aaggccgcga ttcattcggt cgaaaccgga atcatcgact
     1921 cgcatgcttt gatgaagcga ttgtttaacc tgtgccggga tcgtcatgtg atgatggctt
     1981 tcagctcgag cttgagtcac attgagagag ttcgcggttg ctacgagatt ggggtgaacg
     2041 gcgacaacta tcgctttcag tcgcggatcg tagtgaatgc ctctggactt ggaagtgatg
     2101 aggtgctgaa acttattggg ctcgacccgg ttgcaaaggg ctacaagctt ttcttggtga
     2161 aaggaaacta cttcgcctac catcgcccgt cgcccgtctc gcgtttgatt tatccactgc
     2221 ctcatcagga tttgaaaggc ttgggagttc acgccaccct ggaccttgga ggtcgtttac
     2281 gttttggccc ggatgtcgaa aacgtcgaga cgatcgacta tgacgtgtgc gttgatcgaa
     2341 aacagagttt tcacgagtct gcagccaaga tgattgacgg acttgatccg gaggcttttg
     2401 agcctgacat ggcgggtgtc aggcccaagc tcgctgcttc cggctttcgt gattttgtga
     2461 tacaacatga gtctgatgcg ggttttccga atttgatcaa tctggttggc attgagtcgc
     2521 ctggtttaac gagtgcgctg gcgattgcag acgaagtgaa gaaaatggta aagcaactcg
     2581 aggcctgaat atgtgtggaa tcgcgggcat cttcaatctg aatggcgaac cggtttcgcc
     2641 ggtcgtgttg cggaaaatga ccgatgcaat cgcacatcgg gggcctgacg gcgagggttt
     2701 ctactcggac ggattcatcg gactcggaca tcgacgtctg gcgatcatcg acctgtcgcc
     2761 cgcaggtcac caacccatgt tgtcgcgcga tggtcaggtg atactcagct tcaacggtga
     2821 ggtgtacaac tttcaagagt tgagagtcga acttgaagcg ctcgggcaac actttcgctc
     2881 gcgaactgat tcagaagtcg tgttgaatgc ctgggtcgaa tggggtgccg attgtgtacg
     2941 tcgtttcaac ggcatgtttg cgtttgccat ctgggacaaa cgcgagcagg cattgtttct
     3001 cgtccgagat cgttacggag tgaaacccct ctactacgcg tgctggggcc aaacgttttt
     3061 gtttggctcc gagcagaagg cgattctcac tcatccggcg gcgcgtcgag agctcgataa
     3121 gaaggcgtta ctcgaatact tcacctttca gaacatcttc acggatcgta cgttgttacg
     3181 tggagttcgt ttgttgccac cggcgagcat ggtccgtgtt gagctgggga gcgtcagaaa
     3241 gccggacatc aaacggtact gggactactg cttccgtgag cccgaacagc cagctgacct
     3301 gcgagagtat cgtgaagaac ttgaccgctt gattcagcaa gcagtaaggc gacagctggt
     3361 gaccgatgtc gagatcggtt cctatctctc cggcggcatg gactcgggaa cgctgacggc
     3421 gcttgcggcg agagaactcc cctatattaa aacgtttacc tgcggattcg atctcagttc
     3481 cgcttcagga attgaacttg cttttgatga gcgaggcaag gcagaggcca tgtctgcccg
     3541 gttcaagaca gagcattatg aaatggtgct caaggccggg gacatggagc gatgtttgcc
     3601 gagacttgcc tggcatattg aagagcccag agtagggcag agttatccga atttttacgc
     3661 ggctcaactg gcaagtaagt tcgtgaaagt ggtgctctcg ggagccggtg gtgacgagct
     3721 gttcggggga tatccctggc ggtactatcg tgcggccgtt tgcgatgact ttgaacatta
     3781 catcgatcaa tactatgttt tctggcagcg gttgattccc aatacagtca tcaagaaggt
     3841 gtttgcgcct atttgggacg acgtgaaaga tgtttggacg cgagatatct ttcgtaatgt
     3901 ttttttaact catgaaagca aacttgaacg acctgaggat tacatcaacc actctctcta
     3961 ctttgaggca aaaacctttc tgcatggcct gctcgtcatc gaagacaagc tgagtatggc
     4021 tcacagtttg gaaacccgcg taccatttct tgacaacgat ttggtcgatt ttgctatgaa
     4081 atgccctgtc acgctcaagc tgaacaatct cgccgaagtc gtgagaatta acgaaaatga
     4141 agctgcaaac aagaagggaa aatactttca gaaaaccaat gatggcaaac agattctccg
     4201 cgatgtcatg aaaaagtaca tcccagagga agttgtagtt gctgataagc a
//
LOCUS       DAORHD010000266         4250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22396_length_4250_cov_3.570679, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000266 DAORHD010000000
VERSION     DAORHD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22396_length_4250_cov_3.570679"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKO06_06960"
     CDS             complement(<1..243)
                     /locus_tag="PKO06_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69415.1"
                     /translation="MSMMRAEGMPSLLQLLRGVFTWSFFFGSIITVFSFMAVVSAEMQ
                     ALGTYQDLVKARGEVVQINPASFDPTNDGKLVYATGI"
     gene            complement(553..1050)
                     /locus_tag="PKO06_06965"
     CDS             complement(553..1050)
                     /locus_tag="PKO06_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69416.1"
                     /translation="MEPTTTIFVQVIEKPARQLIIKRGKAATHYFEYCEEVGCDVWGV
                     LSSIKGALYEPLGMWLPDGLRPAGTSEYAQGVEVGTDYTGPIPDGMELIALPACHMMV
                     FQSQPYKDEDMGKIIGSMQQAIDSYQPAVFGYEWADQDAPRYQLCPLPERGYIEARPV
                     RALKR"
     gene            1437..2015
                     /locus_tag="PKO06_06970"
     CDS             1437..2015
                     /locus_tag="PKO06_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69417.1"
                     /translation="MFSRWGRILFGMLVVVGVFSGPVAGQSLGAGGADMQAVRAEQQQ
                     KTKAFLDAELKEGQEFMASIAGKPKGEKIVAIRAFKTAQYEKNCAFREERYQERKVFL
                     QGRSGQAGGMNAVMKEKMIDRLEADYREVKEFFAGKHQENMTFLDGVLANVALDGAAL
                     DQALQEFFQEQKKSAQQFMQTKKSFGGGMNRR"
     gene            2196..3893
                     /locus_tag="PKO06_06975"
     CDS             2196..3893
                     /locus_tag="PKO06_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV69418.1"
                     /translation="MGLSSFLVKKADVKKNILIGALVLGVLAGLIFAGIGIGFGAKIP
                     KSQLMFILACVGAGAGFGCVIFWFGLEIILGMVSVVLDDLENKVGLQRQGFESLKTGI
                     NRELDLFDQMFRMVVSILKRTLLISLRLEETSGDLNETAQISSQVIKEIVERVEEINK
                     GAIENAKNLRDTISSLESSFSLSKDISSKLVIAREDSQGVAEIAQGTQASVKTSVERM
                     MKIKELTEGSANLVNDLNDRSKQIGAILTEIANIAEKTNLLALNAAIEAARAGEQGRG
                     FAVVAEEVRKLARGSAESSKKINNLINDILQKTENAAEMMRLNTSKVYEGMDLINAVD
                     KSLSKMVETAMKLNEIIKDISESAERQSKTSDVMYQNVNTISQITDQISHRIQEVAST
                     ALSKTVIVDQTTTSAKELRTLSDIFQNALVPFKFKVEGANKRASVRTETRIPTKFKIL
                     LSGELIDSSFSSSEYDLRGVVTNLSAGGCVIETSEDIEQGKYVIMAFQLGSNFVQGLQ
                     GRLVRLRRAGNDQVGKDNFEAIVSFNNVLPEQEKYIVDFVLEAQRQFEQNLGLKSTD"
     gene            3897..>4250
                     /locus_tag="PKO06_06980"
     CDS             3897..>4250
                     /locus_tag="PKO06_06980"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HNV69419.1"
                     /translation="MARIEKRLLSDSLVADKLVTEEQMRNAFAEQRKTSDCLGLTLLR
                     LKYLTEQQLLEFLTNKLGFNYASMRNTVVDAEIVKLIPENIARKYMCIALLRVKGNLT
                     VAMVDPLDSFIIENLE"
BASE COUNT          984 a         1102 c         1243 g          921 t
ORIGIN      
        1 gattccggtt gcatacacga gtttgccatc gttggtcgga tcgaaggaag ccgggttgat
       61 ttgtaccacc tcgccgcgtg ctttcacgag atcctgatag gttcccaggg cctgcatttc
      121 ggcgctgacc acggccatga atgagaagac ggtgatgata ctgccgaaga aaaacgacca
      181 cgtgaaaacg cctcgcagca actgtaacag cgacggcatt ccttcagcgc gcatcatgct
      241 catgcgtatc ctccacgtcc ccaagactac ccttctgaca caggttattc aacccccgct
      301 ttttctctca ccggagcatc ccatcttgag agcgtttgat cattgatctg cccattatga
      361 gcttcggaat gtcacatgaa cggaacttcc gtcggtgtgt cggtctcgcg ggcgatgcct
      421 gagccagaga cctgcgacat gaacccgtcg gtcatgtcgc aggtcgattg ggacgaattg
      481 tttcttggtc ggattgcgcg agcaggggac tcccttcgat cgtgaaatcg aagggagaga
      541 gaagagacag ttctatcgct tcagggcacg caccgggcgg gcttcgatgt acccgcgctc
      601 aggcaggggg cagagttgat accgtggggc atcctggtcg gcccactcgt agccgaacac
      661 cgccggctga tagctgtcga tagcctgttg cattgatccg atgatctttc ccatgtcctc
      721 gtccttgtac ggctgactct gaaagaccat catatgacac gcgggcagag ctatgagctc
      781 catgccgtca ggaatcggac cggtatagtc ggtgccgact tcgactccct gggcatactc
      841 cgatgttccg gccgggcgca ggccatctgg cagccacatg ccgagcggct catacagcgc
      901 gcccttgatg ctgctcagga cgccccagac gtcgcaaccg acttcctcac agtactcaaa
      961 gtagtgggtt gccgcttttc cgcgcttgat aatcagttgg cgagctggtt tttcgatgac
     1021 ctggacaaag atggtggtgg tcggttccat gatcggtgct tctccttggt ggtaatggag
     1081 gtaatactcg cgagcactgc ggggaaagaa cagcggaatg ggcggggaat cacgccgata
     1141 ggactctgga gtcaggccga actgccgttt gaaggcgcgg gtgaatcctt cgtgcgtgct
     1201 gaatgccgta tcgagactcg atcaagtcct gcattctctg caccgcgtca tacttttccc
     1261 gcatgcatcg tttcctcctg cctgtgttgt accagatcga gctgcgtttc ctcttgaccg
     1321 gaattgcgaa ccttatcaat cataagttcg ggcgtccggc gtttttttcg ggatggcgac
     1381 ccgcggaaaa ccgtggtaat gatacctgcg aatctgacaa gacagggaga gccgaaatgt
     1441 tttcacgctg gggacggatt ctattcggga tgctggtggt cgtcggggtg ttttccgggc
     1501 cggtggcggg gcagtcgctg ggagcgggtg gagcggacat gcaggcggtt cgcgccgagc
     1561 aacagcaaaa aacgaaagcc tttcttgatg ccgaattgaa agaagggcag gagttcatgg
     1621 cctcgattgc cggaaaaccg aagggagaga agatcgtggc gattcgcgca ttcaagactg
     1681 cacagtacga gaaaaactgt gcctttcggg aagagcgcta tcaggagcga aaggtgtttc
     1741 tgcaaggtcg atcgggtcag gctgggggca tgaacgcggt gatgaaggaa aaaatgattg
     1801 atcggctgga ggcggactac cgtgaggtga aagagttttt cgccggcaag catcaggaaa
     1861 acatgacttt tctggatggc gtgctcgcaa atgtcgctct cgatggagct gctctggatc
     1921 aagcgcttca ggagttcttt caggaacaga aaaagtcggc acaacagttc atgcagacga
     1981 aaaaatcgtt cggtggtggc atgaaccggc ggtagacttt tccgccgaga ttcagtagat
     2041 tactatactg aattttcggg tttctcccga tcaaggaatg ccggaatgtt tccggcgggt
     2101 ttgttttcgg atcaactgac tgaatcgcac catgaaggca ggccgaatga ctgaacgcac
     2161 gaatgccatg gaaagcttga ggtagagctt ccaccatggg tctctcgtct tttctcgtca
     2221 aaaaagccga cgtcaagaag aacatcctga tcggcgctct ggttctcgga gtgctggccg
     2281 gtcttatttt tgccggcatc ggcatcgggt tcggagccaa gattccgaag tcgcagctga
     2341 tgttcattct ggcatgcgtg ggtgccggtg cgggcttcgg gtgcgtgatc ttctggttcg
     2401 ggctcgagat cattctcggc atggtcagcg tcgttctcga cgatctcgaa aacaaggtcg
     2461 gcctgcaacg gcaggggttc gaatcgctga agaccggcat caatcgcgaa cttgacctgt
     2521 tcgatcagat gttccgcatg gtggtttcga ttctcaaacg cacgctgctg atctcgctgc
     2581 gactcgaaga aacgtcggga gatttgaacg agaccgcgca gatcagttcc caggtcatta
     2641 aagagatcgt cgagcgagtg gaagagatca acaaaggcgc gatcgaaaac gccaagaacc
     2701 tgcgcgatac gatctcgtcg ctcgaaagca gcttcagcct gagcaaggat atcagttcga
     2761 agctggtcat cgcccgcgag gacagtcagg gtgtggccga gattgcccag ggcacgcagg
     2821 cttctgtgaa gacgtcggtc gagcgcatga tgaagatcaa ggaactgacc gaaggttcgg
     2881 ccaatctggt caatgacttg aatgatcgca gcaagcagat cggcgccatc ttgactgaaa
     2941 tcgcgaacat tgctgaaaag acaaacctgc tcgcgttgaa cgccgctatt gaagccgctc
     3001 gcgctggtga gcagggccgt ggtttcgcgg tcgttgccga agaagtgcgc aagctcgcgc
     3061 gtggttcggc cgaatcgtcg aagaagatta ataatctgat caacgacatt ttgcagaaaa
     3121 ccgagaatgc cgccgaaatg atgcgcctga acacatcgaa ggtgtacgag ggcatggatc
     3181 tcatcaacgc ggttgacaag agtctgagca agatggtcga aacggccatg aaactgaacg
     3241 agatcatcaa ggatatcagc gagtcggccg agcgccagag caaaacctct gacgtcatgt
     3301 atcagaacgt gaacacgatt tcgcagatca ccgaccagat ttcgcatcgc attcaggaag
     3361 tggcgtcgac ggcattgagc aagacggtca tcgtcgatca gaccaccacc tcggccaaag
     3421 agctgcgcac cctgtcagac atcttccaga acgcgcttgt gcccttcaag ttcaaggtcg
     3481 agggtgcgaa caagcgtgct tcggtgcgca cggaaacccg cattccgacc aagttcaaga
     3541 tactgctctc gggcgagctc attgacagca gcttcagctc gagtgaatac gacctgcgcg
     3601 gggtggtgac gaacctctcg gccggcgggt gtgtcattga aaccagtgaa gacatcgagc
     3661 aggggaaata cgtcatcatg gccttccagc tcggcagtaa cttcgtgcag gggctgcagg
     3721 gtcgcttggt gcgcctgcgt cgtgccggca acgatcaggt cggcaaagac aatttcgaag
     3781 ccatcgtgtc gttcaacaac gttcttcctg aacaggaaaa atacattgtc gatttcgtgc
     3841 tcgaagcgca gcggcagttc gagcagaatc tcggcctcaa atccacggac taacctatgg
     3901 cacgcattga aaaacggctg ctcagcgact cgctggttgc cgacaagctc gtcactgaag
     3961 aacagatgcg gaacgcgttt gccgagcagc gcaagaccag cgattgtctc gggctgacgt
     4021 tactgcggct gaaatacctg accgaacagc agctgctcga gtttctcacc aacaaactcg
     4081 gcttcaacta tgccagcatg cggaacaccg ttgttgatgc cgagatcgtc aagctcattc
     4141 ccgagaatat cgcgcgcaag tatatgtgca tcgcgttgtt acgggtcaaa ggcaacttga
     4201 ccgttgccat ggtcgacccg ctcgacagct tcatcatcga gaatctcgag
//
LOCUS       DAORHD010000267         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22422_length_4245_cov_2.978282, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000267 DAORHD010000000
VERSION     DAORHD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22422_length_4245_cov_2.978282"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1043)
                     /locus_tag="PKO06_06985"
     CDS             complement(198..1043)
                     /locus_tag="PKO06_06985"
                     /inference="COORDINATES: protein motif:HMM:NF039494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV69420.1"
                     /translation="MIRNRLLVLLLACFAVVLSPSTSHAAASDPMKALQNLELPVSRT
                     AQAGTENAGTSTPANAAGGGQYTVVPGDCLWNIAAKYLGNGSRYMELVEANKSKYPSL
                     VKNPNLIYPGWVLTIPGQSGNLNPGNSTASRPPSSSPGSSPGNSSPSNSPAANPNVPA
                     PAAGASGGRALYSWLQRAGLSGEKLKVAWAVGMAESGGNPRAHNGNAGTGDNSYGLYQ
                     INMINSLGPARRRQYNLSSNEELFNPETNIRVMLHMSNNCTNWQPWSAYKNGSYRRFL
                     SQFPP"
     gene            1641..2807
                     /locus_tag="PKO06_06990"
     CDS             1641..2807
                     /locus_tag="PKO06_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69421.1"
                     /translation="MALNTGVPEEIRCYAIMAFGKTPGAQSFPVMQRLVWDPIPAVRY
                     TCAGMLSHFPGIMALPLLRRLLKDENPFVRSAAVHSIGSWKTPHILASLFRALADKSK
                     LVRQTVRETIRTFLEETLVPTFQKAMRRMRPVAVLRGIKFLWESQHPEAVPLLNHVLR
                     TSSGPTRRVLLRFAMKTGHPSLMYDIKSAIRTTETETLIDLQNYLEYCHPASITFTTL
                     FTYVKKLEQLTPLARSAHLKKLLDHADAHVRTMAVRSLGDINLEGLYDDLVKLYEREQ
                     NTSVRVCILEIFPKFREEAEPVLLTALSTDEPAILQAAIRGLSEVGGQHALDRLAQVA
                     KARIPATAKFFVECAIEKISERLNASRTTSHGERVASDDFLRAIEPSLDGPGVR"
     gene            2846..>4245
                     /locus_tag="PKO06_06995"
     CDS             2846..>4245
                     /locus_tag="PKO06_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69422.1"
                     /translation="MTSPVLPQEDTLTTQSTIYTLRKRWKLLVAIQLLFVLGGFYVYY
                     ITPKVYSISSSIGLDRPYAASNWQNYTVEKENQTIVRLLTSEDLLERAIASLSLPPET
                     FREVKVNAKIPRDSNVVNVELEDYVPSRMPVLFQTMITLAEARASENIGSGWNTKVDF
                     LRKQIKSAEEKLSEVDKQLQVFIQQEGVLGVADKLKEISQEAQALKQEQRQNEVELGI
                     INEALDELRKKAKEMSKVRSSSSYDFSELNDLQRELIQLKREQVRLASLYQSDHPEMR
                     TNADQIAMIKKMYDRKMKEVQSDQGRTDSSGTKDAHIFQRVVELETQQKSLAQKIRLA
                     TTLGDEKTQAFKAMASKLEEYSSLARKQKINEEMHSQLLAKLNETELEKDVKNVTFKV
                     LSRPVVPTTPIRPRFLWTILWALAIGTVLGFIVIHTQETIASTFQTPEEAAEALGLTC
                     LGIIPDFKHLFAKPYD"
BASE COUNT          992 a         1117 c         1242 g          894 t
ORIGIN      
        1 aggaaccagg acatgctgaa gatatctgac acgatatctt cagcatgtcc tggttcctga
       61 ctgctcccgg tccggcccag gcgcagagag tgcataaaga ggcgaagaga acggtcaaca
      121 tcacgatctt gaatttcata ttccttgctc cgcaatagtc ctagcttgaa gtagatgaaa
      181 acagcctaaa tagatggtca gggcggaaac tgagagagga agcgccgata cgacccattc
      241 ttgtaggcgc tccacggctg ccagttcgtg cagttattac tcatgtgcag catgacgcga
      301 atgttggttt cgggattgaa cagctcttcg ttcgaactca ggttgtactg acgacgtcgg
      361 gctggtccga ggctgttgat catattgatc tgatagagcc cgtaggagtt atcaccggta
      421 ccggcatttc cgttatgtgc ccgaggattg ccgcctgact ccgccatccc gaccgcccag
      481 gcgaccttga gcttttcacc tgacagtccg gcacgctgga gccaggaata caaggctcga
      541 cctccactgg cgccggcagc aggagcgggg acatttggat tggcggccgg agaattcgag
      601 ggagaagagt tccctggaga cgatcctggg gatgacgagg gaggtcgaga agccgtcgag
      661 ttgccggggt tgagatttcc gctttggccg gggatggtaa gaacccaccc ggggtagatc
      721 agattcggat tcttcacgag gcttggatac ttggatttgt tggcttccac cagttccatg
      781 tatctgctgc cattgccgag atacttggcg gcgatgttcc agagacaatc gcccggcact
      841 acggtgtact gtccaccgcc agcggcgttg gctggcgtag aggtgcccgc gttctcggtc
      901 cctgcctgcg cagttctgct gaccgggagt tcaaggttct gcagcgcttt catggggtcg
      961 cttgcggccg cgtgtgacgt cgatggcgat aacacgacgg cgaaacaggc cagcagtaac
     1021 accagcagtc gattccgaat cataaaaagc ctccccgaaa gtgttccgat acggtgcagt
     1081 gggttacatc tgattacaac gagaaaggaa agaattttgt gacagatacc gggtgtttct
     1141 gcgcgcggtt tcctccctgc agtatagcgg gtgagacagg aaggctcagt ttttctcggt
     1201 aatcacccga tgataggact ttgttatcag aaagattttc agtcgactga gtcagtatct
     1261 cgagtggatc tgacagcggg agaacttgaa tgagttctac atctcttctc ttcgttccct
     1321 tcatccggca cctcgtcaaa aagatccgag gtgaagtgac acccgtggta gcgcccgtca
     1381 cactcctgca accaacgcct ccgacgtaat ctgagccggt cgaccgtgta gacgatgccg
     1441 gacaacttct gcaggagcgg gaggcggggg cagatggttc ttccgcgaat ccgcagacgg
     1501 tcgtgtttcc ggaagagcgc gcgccgaggc cactgcccat caatccgaaa attgcggaga
     1561 gtgccccgac gaaggatctg gttacgacga cgtctgtcgc ccttgagctc tccgcgcagc
     1621 gatgccagag tcatgaggat gtggctctga acacgggagt accggaggag attcgctgct
     1681 acgcgatcat ggcgtttgga aaaacgccgg gcgctcagag tttcccggtc atgcagcggc
     1741 tggtctggga tccgattccg gcggtacggt atacctgcgc cggcatgctg tctcacttcc
     1801 caggaattat ggctcttcca ctgctacggc ggcttctgaa agacgaaaat ccgttcgtgc
     1861 gatctgcggc ggtgcactcg atcggctcgt ggaaaacgcc acacattctg gcgagcctgt
     1921 tcagagctct cgcggacaag tcgaagctgg tacggcagac cgtccgggaa accattcgaa
     1981 cgttcctgga agagacgctg gttccaactt ttcagaaggc gatgcgccga atgcgtcctg
     2041 ttgctgtact gcgtggcatc aagttcctgt gggaatctca gcaccccgaa gccgtgccgc
     2101 tcctgaatca cgttctgaga acgtcatccg gaccaacccg cagggtgttg ctgcgttttg
     2161 ccatgaaaac cggccacccg tcactgatgt acgatatcaa gagtgccatc aggacgacgg
     2221 aaaccgagac tctcattgac cttcagaact acctggaata ctgccacccg gcgagcatca
     2281 cttttaccac gttgtttacc tacgtgaaga agctcgaaca actgacgccg ttggcgcgct
     2341 cagcccatct caagaagctc ctggaccatg cggacgctca cgtccgaacg atggcggtgc
     2401 gttctctggg agatatcaat ctcgagggct tgtacgatga tctggtgaag ttgtatgaga
     2461 gggaacagaa cacgagtgtg cgggtgtgca tcctggagat cttcccgaag tttcgggaag
     2521 aggcggaacc tgtgcttctg acagcgcttt cgacggacga gccggcgatt ctgcaggccg
     2581 cgattcgcgg cttgtcggag gtcggggggc aacatgcact ggatagattg gctcaggtgg
     2641 cgaaagcccg gattcctgcg acggccaaat ttttcgtcga atgtgcgata gaaaagatca
     2701 gcgagcgcct caatgccagc cggacaactt ctcatggaga gagagtggcc tctgacgact
     2761 ttctacgggc tatcgaaccg tcgctcgatg ggcctggagt caggtaaatg ctggacagat
     2821 gcgagaaagt gggagggaaa ccggaatgac ctcacccgtg ctgccgcagg aagataccct
     2881 cacaactcaa tcgaccatct acacgcttcg caaacgctgg aagctgctgg tggccataca
     2941 attgctcttt gtactgggcg gcttctatgt ctactacatc acgcccaaag tctattcgat
     3001 ttcgagttcc atcggtctcg atcggccgta tgcagcctca aactggcaga actatacggt
     3061 ggaaaaggaa aaccagacga tcgtccgatt gctcacctct gaagacctgc tcgagcgggc
     3121 gattgccagt ctgtcgctcc ctccggagac ctttcgcgag gtgaaggtga atgcgaagat
     3181 tccccgcgat tccaacgtgg tgaacgtgga actcgaggac tatgttccca gtcgaatgcc
     3241 ggttctcttt caaaccatga tcaccctggc tgaagcgcgt gcctcggaga acatcggctc
     3301 ggggtggaac accaaggtcg attttctgcg caagcagatc aagagcgcgg aagagaagct
     3361 gtctgaagtg gacaagcagt tgcaggtgtt cattcaacag gagggagtcc tgggggttgc
     3421 ggacaagttg aaagagatct cgcaggaagc ccaggcgctg aagcaggaac agcggcagaa
     3481 cgaggtcgaa ctgggcatca tcaatgaagc gctcgacgag ctgcgaaaaa aggccaagga
     3541 gatgtccaag gtcagaagct cgagcagtta cgatttttca gagttgaacg atctgcaacg
     3601 tgagctcatt caactgaaac gcgagcaggt gaggcttgcc agtttgtacc agtcagatca
     3661 tccggaaatg cgcaccaatg ctgatcaaat agcgatgatc aagaaaatgt acgatcgaaa
     3721 gatgaaagag gtgcagtcgg atcaggggcg cacggattca agcggcacga aggatgccca
     3781 catctttcag cgcgttgtcg agctcgagac ccagcagaag tcgctggctc agaagatccg
     3841 tctcgcgacc actctcggcg acgaaaagac acaggcgttc aaagcgatgg ccagcaagct
     3901 cgaggagtat tcttccctgg cgcgcaaaca gaaaatcaac gaagagatgc acagtcagct
     3961 gctcgcgaag ctcaacgaga cggaactgga gaaagacgtc aaaaacgtca ccttcaaggt
     4021 attgagtcga ccggtggttc cgacgacgcc gattcgacct cgatttctgt ggaccattct
     4081 gtgggcgctg gcgatcggaa cggtactggg tttcatcgtg attcataccc aggaaacgat
     4141 cgcctcaacg ttccagacgc ctgaggaggc ggcggaagcg cttggtctga cctgtctcgg
     4201 gatcattcca gacttcaagc atctgttcgc caaaccgtat gacaa
//
LOCUS       DAORHD010000268         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22448_length_4242_cov_3.098877, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000268 DAORHD010000000
VERSION     DAORHD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22448_length_4242_cov_3.098877"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="PKO06_07000"
     CDS             complement(<1..217)
                     /locus_tag="PKO06_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69423.1"
                     /translation="MSNHFVRRSLVLLFVLLLAQRATAAVVNIGKYRVNVDVRTSLMV
                     IIPHALIQGDVKGSLIKIRVSAPGYLPQ"
     gene            291..2204
                     /locus_tag="PKO06_07005"
     CDS             291..2204
                     /locus_tag="PKO06_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV69424.1"
                     /translation="MNILSLEAIGKRYSAAPLLESVTFGLEHDERMGIIGRNGSGKTT
                     LLRIIAGIEPPDQGRVVLANHRIIASVPQNPTFREGMSVLDAVFDQADERLLRLRAYH
                     QACLALEQGSDHQEKLLARVTELGAELDLRGGWDLEACAKSILHQLGLEDLGKSVDTL
                     SGGQRKRLALARALVIRPDLLILDEPTNHLDPDVITWLEEYLEYYSGALLLVTHDRYF
                     LDRVVNRMLEIEKGRVQRFEANYTRYLEIKEQQSLQRESEAVKREGLIRRELAWLRQG
                     AKARSTKQKAHVDRATALISAPRDRPERELEMSSASSRLGNKIIELTNLTKTYGNRTI
                     IDRFSYRLKHGDRIGIIGPNGSGKTTLLEMVAGRLAPDAGQRDVGQTVVIGYYDQESR
                     ALENELRVIDYVRKTAEHVNTGEGGTVSAGQMLERFLFSPTQQSAYISTLSGGERRRL
                     YLARLLMGAPNVLLLDEPTNDFDIVTLMALEAWLDVYSGCLLVASHDRYFLDRTVTQL
                     FHIESGGHLRGYPGNYTTYLEMRRSVETTALETGATKSAKPSDSAATPATSAAPQKSR
                     SQKRTFKEQREYEDLEKRIAAAESRKAQIENLLANPNGDFSALATLAQELHDLNGQID
                     REMNRWAELAEKE"
     gene            2213..2434
                     /locus_tag="PKO06_07010"
     CDS             2213..2434
                     /locus_tag="PKO06_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69425.1"
                     /translation="MDRQRVIIYSVVIVLVLGILLLNAHWGRQTAPPQDDFPSFSDRS
                     QQSEFERGAGTMRDAKAFKEWAKKNLPDD"
     gene            2669..3460
                     /locus_tag="PKO06_07015"
     CDS             2669..3460
                     /locus_tag="PKO06_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV69426.1"
                     /translation="MEIKNSVAVITGGASGIGEAVARSLAAKGAKIVIGDMDAKGLDR
                     VASEITQAGGQVAFAKCNVTSDEDVANLMDLAIEKFGQINICVASAGIIKDGLMINLD
                     KDTGKVKKAMTTDQFKAVVDVNLVGSFVTIREAAIRMVNNGYKGLLVPISSVNKAGQT
                     GQLNYSSTKAAIALWPKIIVGEFQMRGVTNVRCVAIAPGYVGTPMVKGMNQEALQGIL
                     KGVHIGRLIEPEEIADIIAYAAQNEAIDATCLEITGGLISNGLAK"
     gene            3475..>4242
                     /locus_tag="PKO06_07020"
     CDS             3475..>4242
                     /locus_tag="PKO06_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanoyl-CoA acyltransferase"
                     /protein_id="HNV69427.1"
                     /translation="MSKVSIIGAYNTKFGSLIEVKDMISFKKKIDVKSFYDLIIEAGQ
                     GAIKDSGLDPKDIDAVWIGSCSPSRFVNQEHVAPIAVQIDPSLRFKPMTRTECACASS
                     SVAIYDATYAIEAGRFKNVLVIGVEKMNLLDTPSMTATLATCSYWPEEGSKGGTFPGL
                     FAELAKAYQEHYKLSDTELRTQLAHIAALCYRNAATNPLAHIQNGPATAAEILAMPTT
                     GRGSNLCIAAPLHLHDCSLVTDGAAAVQPVHSRALSDE"
BASE COUNT          977 a         1269 c         1193 g          803 t
ORIGIN      
        1 tctgtggcag atacccgggc gctgaaaccc gaatcttgat caggcttccc ttgacatcac
       61 cctggataag ggcatgggga atgatcacca tcagactcgt tctgacatcg acgttgaccc
      121 gatactttcc gatgttgacc acggcagccg tcgcacgctg cgcgaggagc agcacaaaga
      181 gaagcacgag cgagcgacgc acaaaatggt tcgacacctg tgaatctctc aaggtatggg
      241 caactctgtt catatagaca atgatatcat gtgcgtactg cgtttcaaac atgaacatac
      301 tctctctcga agctatcggc aaacgttact ccgccgctcc attgctcgaa agcgtgacct
      361 tcgggctcga gcacgacgag cgcatgggca ttatcggtcg caacggcagc ggcaagacga
      421 cgctgttgcg catcatcgcc gggatcgagc cccctgacca ggggcgggtc gtgctggcga
      481 accatcgcat catcgcgtcg gtgccacaga acccgacttt tcgggaaggc atgtcggtgc
      541 tcgatgcagt gttcgatcag gccgacgaac ggctgctgcg cctgcgagcc tatcatcagg
      601 cctgcctggc cctcgaacag ggaagcgacc atcaggaaaa gctgctggcg cgggtgacgg
      661 aactcggtgc ggaactcgat cttcgcggcg gctgggatct cgaggcctgt gccaagtcga
      721 ttcttcacca gctcgggctc gaagatctgg gaaaatcggt tgacacgctc tcaggcgggc
      781 agcgcaaacg tctggccctg gcgcgagctc tggtgattcg ccctgatctg ctgattctcg
      841 acgaaccgac caatcatctc gaccccgatg tgatcacctg gctggaagaa tacctcgaat
      901 actattcagg ggcactgctt ctcgtgaccc acgaccggta ctttcttgac cgggtggtca
      961 accgcatgct cgaaatcgag aaggggcggg tgcaacgatt tgaggccaac tacacccgct
     1021 atctcgagat caaggagcag cagtccctgc aacgcgagtc cgaggcggtc aagcgcgaag
     1081 gactcatacg ccgcgaactt gcctggctca gacagggagc caaagcccgg tcaaccaagc
     1141 agaaagcgca tgtcgaccgg gcgacggccc tgatctcggc acccagagat cgccctgaac
     1201 gagaactcga gatgtcttcc gcctcttcac gtctcgggaa caagatcatt gaactgacga
     1261 atctgaccaa gacgtatggg aatcgcacca tcatcgatcg gttttcctac cggctcaaac
     1321 acggtgatcg tatcggcatc atcggcccga acgggtcagg aaaaacaacc ctgctcgaaa
     1381 tggttgccgg acgtctcgcc ccggatgctg gccagcgcga cgttggccag accgtcgtca
     1441 tcggctacta cgatcaggaa agccgggcac tggaaaacga gttgcgggtc atcgattatg
     1501 tgcgcaagac ggcagaacac gtcaacacag gcgaaggtgg aacggtgtcg gccgggcaga
     1561 tgctcgagcg ctttctcttt tcgccaacgc aacaaagcgc gtatatctcg actctttcgg
     1621 gcggcgaacg tcgccggctg tatctggcgc gcctgttgat gggggcgccg aatgtgctgt
     1681 tgctcgacga accgacgaac gattttgaca tcgttacgct catggcgctc gaagcgtggc
     1741 tcgatgtgta ttccggttgc ctgctggtcg cgtctcacga ccgctacttt ctcgaccgta
     1801 ccgtcaccca gctgtttcac atcgagtcag ggggccactt gcgcgggtat ccgggcaact
     1861 acacgacata tctcgaaatg cgccggtcag tcgagacaac ggcactcgag accggggcca
     1921 ccaaatccgc caaaccatct gacagcgcgg caacacctgc gacatcagcg gcaccgcaaa
     1981 agtccaggtc gcagaagaga acattcaagg aacagcgcga gtatgaagat cttgaaaagc
     2041 gtatcgcggc cgccgaatcg cgcaaagccc agatcgaaaa cctgctggcc aacccgaacg
     2101 gcgacttctc cgctctggcg acactcgccc aggaactgca cgacctgaac ggacagatcg
     2161 accgcgagat gaaccgctgg gccgagcttg ccgagaagga ataggagcac ctatggatag
     2221 gcaacgagtg attatctaca gtgtcgtgat cgtcctcgtg ctcggaattc tgctgctgaa
     2281 cgcgcattgg ggacgacaga ccgcgccacc gcaagacgac tttccgtcat tttccgatcg
     2341 ctctcagcag tcggagttcg aacgtggagc cggaaccatg cgcgatgcca aggcattcaa
     2401 ggaatgggcg aagaagaacc ttcccgacga ctgaccgtcg acacgatcct ccgcatctct
     2461 ccacgaaacg ctttggggaa tctcgaccga tcgacactct ccccggcatg tcggccatga
     2521 gagccgattt cgcgccattt cccactggtt atacaacgca agtgcgaaat atttctcatg
     2581 acgcacttgc gaaataccga tagatatcgt atagtcaatt ccggtaacct gagtttcatt
     2641 taccactaca ttcaagcgag gtacagacgt ggaaatcaag aattccgttg cagttatcac
     2701 gggtggggca agcggtatcg gcgaggccgt ggcgcgctct ctggccgcga aaggcgcgaa
     2761 gatcgtcatc ggcgatatgg atgccaaggg ccttgaccgg gtcgccagcg agatcactca
     2821 ggcgggtggt caggttgcgt ttgcgaaatg caacgtgacc agcgatgaag atgtcgccaa
     2881 cctgatggat ctggcgatcg agaagttcgg gcagatcaac atctgcgtcg ccagcgccgg
     2941 catcatcaaa gacggcctga tgatcaacct cgacaaggat acgggcaaag tgaagaaggc
     3001 catgactact gaccagttca aagcggtggt cgatgtcaac ctcgtgggca gcttcgtcac
     3061 gattcgagaa gcagcgattc gcatggtaaa caacggatac aaagggctgc tggttccgat
     3121 ttcgtcggtg aacaaagccg gtcagactgg tcagctcaac tactcctcga ccaaagccgc
     3181 gattgccctg tggcccaaga tcatcgtcgg cgagttccag atgcggggtg tcaccaatgt
     3241 gcgctgcgtc gccatcgccc ccggttatgt cggcacgccg atggtgaaag gaatgaacca
     3301 ggaagccctg cagggcattc taaagggcgt gcatatcggc cgcctgatcg agcccgaaga
     3361 gattgccgac atcatcgcgt atgcggctca gaacgaagcg atcgacgcga cctgcctcga
     3421 aatcaccggc ggcctgatct cgaacggcct ggccaagtaa caggagcacc aaccatgagt
     3481 aaagtcagca tcatcggcgc ctacaacacc aagttcggtt ctctgatcga agtcaaagac
     3541 atgatctcgt tcaagaagaa gatcgacgtc aagtcgttct atgatctcat catcgaagcc
     3601 gggcagggcg cgatcaaaga ttcaggtctc gaccccaagg atatcgatgc cgtctggatc
     3661 ggctcgtgca gcccgtcacg attcgtcaat caggaacacg tggcccccat cgccgtgcag
     3721 atcgacccgt cattgcgctt caagcccatg acccgcacgg aatgcgcctg cgcgtcgtct
     3781 tccgtcgcta tctatgatgc gacgtatgcc atcgaagccg gccgcttcaa gaacgtgctc
     3841 gtgatcggcg tcgaaaaaat gaacctgctc gacactccct cgatgaccgc cacgctggcc
     3901 acctgttcct actggccgga agaaggttcg aagggcggca ctttcccggg cttgttcgct
     3961 gaactggcga aagcctatca ggaacactac aagctctctg ataccgaact gcgcactcag
     4021 cttgcccata tcgccgccct ctgctatcgc aatgccgcca cgaatccgct ggcgcacatt
     4081 cagaacggcc ccgcaactgc cgccgagatt ctggccatgc cgaccaccgg ccgcggatcg
     4141 aacctgtgca tcgccgcacc gctgcacctg catgactgct cgctggttac cgatggcgcc
     4201 gccgccgttc agcccgtcca cagtcgggct ctgtccgatg ag
//
LOCUS       DAORHD010000269         4221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22600_length_4221_cov_3.291167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000269 DAORHD010000000
VERSION     DAORHD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22600_length_4221_cov_3.291167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4221
                     /locus_tag="PKO06_07025"
     CDS             <1..>4221
                     /locus_tag="PKO06_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69428.1"
                     /translation="WVRINDIVTFRVQLASSSAVIHDGEAVTINLSSLGSGYSTAQTM
                     TYDSLNAYTHSVTIPAGTLNNFTPFTFLAKDNAENPVSGSIIVKIDNYAPVVGPMTVR
                     YLVDYTNVDTVNLGDRMEFLVPCSDPDGGSCTIDLSYIGSGAFHYMDYDPTLQVYSTV
                     VDTASASNENSAYVFRARVTDKAGNLTSSLSGPYPVDCVPPVVYWASATYQELIGSST
                     VVNVGDKITISASVQNSRLDGGVPVVNLAEFGLSSAQQLYDDGAHGDGAPNDGIWAYQ
                     FTVAKGTINADYRSFVVAVTDNAGNSASRTTNSLFIDNQPLVINYYLASIDTDANGNA
                     VADLDGSYGVAHVVTTDTVKLSIRVTGADADFNLCTVDLTKLGYTDVASTMPILSAIP
                     GQRDYQNIFTLREGSTNREMVTFSTLVTDINGNQTFATATPQLMVDNFPPLITQMFPI
                     TFVTDAIPTGEANLGDKIQFKVRVSNHDNNPPEIDFSNLLSANGLSSTTPIVQMTGGP
                     TEYTYTWTTPEGLGSLASLPILVWDASKNLRLGYTNAIRFLSTKPNIMGYPNSAAVLS
                     SDTYPTVPNNICNPTDQVTITAVLNKAFNPTNTPPATVCVDIRSITNDSSDDAFGFID
                     GDPKTYWAPLTYVLPPVSGAGNWVYRTTFTAGPQGRTDITNASFSVKVLHPDYSSIAM
                     ATGTIYCDPLNPFGIDTLVPRIYQTQIRITNENGDNISSTVANIGDVITVYANIADYA
                     DPGSITARLLTAGSVLIEEATMNWLTGDQYYASFVVATNTVVPWNISLNGNPATDYLR
                     YEVHVTDDAQNYHGVAGRSDVGQPKNFVVDNTPSDILGFQFLLNPNHPFAWVGNVGSA
                     PVLLTGQYDNLRVDGLGASITVNSGCRQAWVDLTNIQATSTFMLINTTVAGGQTTGTT
                     PPAGYVKLATVSLELEPALGPGTYSFNITVVDTAGNRMASSAEQAFDTTRPYLMRAEY
                     DGSYLTLQFNEPLKVGDYTLTMPERFDQTKIRIGNKSNLELVGPDSVVALSALDLVQD
                     PYADTDTIRIALASGTRATIADWGNVSIWMSMGATGPTGEGASVSVDLASNWVNPIKQ
                     SAGRVINVPVPYAIRPKIIGGAYDAVNVPSILYIDFDKPMATDTMTNFTLPAFAVVRE
                     RRNDLRSVYYPWRYNPVIGSDTVGVPSSTTRLAINLSPEAQDWNAIKFGRLASQLNYA
                     VSTATPPLVRDEKGNGVQPISFDNAVAAALSPENRSFEVEPTLGNEIVLHLASKTLTI
                     PLNRRALIWGGEFADNTPKFNPDFNRLNVIPSRVFLYKRADMTGSSISLNRAANNLPA
                     WPIYNTSFASTTVIIPLSDDDIRDVLSWNSGSLYVAMNEGAFKDLWGNNSSRYPSSTT
                     TAAPVTVVKPVGYPGPQVISVAVSDPTPT"
BASE COUNT          940 a         1197 c         1123 g          961 t
ORIGIN      
        1 tgggtgcgta tcaacgacat tgtcacattt cgcgtacagc tagccagcag cagtgcggtt
       61 atccatgatg gagaagccgt cactatcaat ctctcgtctc ttggcagcgg gtactcaacg
      121 gctcagacga tgacctatga cagtctcaat gcctacacgc actcggtaac cattccggcc
      181 ggcacgttga acaacttcac gccgtttacc ttccttgcca aggacaatgc cgagaatccg
      241 gtgagcggca gcattatcgt gaagatcgac aactatgctc cagtcgttgg tccgatgacg
      301 gttcgctatc tcgtcgatta cacgaacgtc gacaccgtca atcttggcga tcgcatggaa
      361 ttcctggttc cgtgttccga ccctgatggc ggaagttgta cgatcgatct gtcctacatt
      421 ggcagtgggg cttttcatta catggactat gacccgactc tgcaggtcta ttccaccgtc
      481 gtcgatacgg cttccgcttc gaacgaaaac agcgcgtatg tgtttcgtgc ccgcgtgacg
      541 gataaagcgg gtaacttgac cagttctctt tccggaccgt atccagtgga ttgtgtgccg
      601 ccggttgtct actgggcttc tgcgacgtat caggaactga ttggcagttc gaccgtcgtg
      661 aatgtgggcg ataagatcac gatttcagcg agtgttcaga acagccgcct cgatggagga
      721 gtgccggtcg taaacctggc ggaattcggc ctgtcgagcg cgcagcagtt gtacgatgat
      781 ggcgcacatg gcgatggcgc tccgaacgat ggaatctggg cataccagtt tacggttgcc
      841 aagggaacga tcaacgcgga ttatcgcagc ttcgttgtcg cggttacgga caacgcgggt
      901 aacagcgctt ccagaacgac gaattctctg ttcatcgata accagccgct ggtaatcaat
      961 tactacctgg cttcaatcga cacggatgcc aacgggaatg cggttgccga tctcgatggt
     1021 tcctacggcg ttgcgcatgt ggtcactacg gatacggtca aactgtccat tcgcgtgacc
     1081 ggtgccgatg ccgatttcaa cctctgcact gtcgatctga ccaaactcgg gtacacggac
     1141 gtggcgagta ccatgccgat tctgtccgcg attcctggtc agagagacta tcagaacatt
     1201 ttcacgctcc gcgagggttc gacgaatcgt gaaatggtca ccttcagcac tctggtgacc
     1261 gacatcaacg ggaaccagac gtttgccacg gcgacgccac agttgatggt cgacaacttt
     1321 ccgccactca tcacgcagat gtttccgatc accttcgtga cagacgcgat tccaacgggt
     1381 gaagcgaacc tcggtgacaa gattcagttt aaagtccgag tcagcaatca cgataacaat
     1441 cctccagaaa tcgatttcag caatctgttg tcagcaaacg gtctgtcgtc gaccaccccc
     1501 atcgttcaga tgaccggcgg cccgacggag tatacctaca cctggacgac tccggaagga
     1561 ctcggatcgc tcgcctctct gccgattctg gtctgggatg cttcgaagaa cctgcggctt
     1621 ggatatacca acgcgatccg ctttctctcg accaagccca acatcatggg gtatcccaac
     1681 agcgcagcgg tattatcttc agatacgtat cccaccgtac cgaacaacat ctgtaatccg
     1741 acggatcagg tgacaattac cgcggttctg aataaagcat tcaatccgac aaacactcct
     1801 cctgccacgg tgtgcgtcga cattcgcagt attacgaatg acagcagtga cgacgctttt
     1861 ggatttatcg atggtgaccc gaaaacctac tgggcacctc tgacctatgt gttgcctcca
     1921 gtcagtggcg caggcaactg ggtctaccga acgacgttca cggctggacc acaggggcgc
     1981 accgatatca caaatgccag cttctcggta aaggtgcttc atccggatta ttcgtcgatt
     2041 gccatggcaa ccggaaccat ctactgtgat cccttgaatc cctttggtat tgatacgctc
     2101 gtacctcgca tctatcaaac tcagattcgc atcactaacg aaaatggtga caatatctct
     2161 agtacggtag ccaacattgg agacgtgatc accgtctacg ccaacatcgc ggactatgca
     2221 gacccgggtt cgatcacggc tcgtctgctg actgccggat cggtgctgat tgaagaagcg
     2281 accatgaact ggttgaccgg tgatcagtac tacgcgagct tcgtggttgc gacgaacacg
     2341 gtcgttccct ggaacatctc tttgaacgga aaccccgcga ccgactacct gcgctatgaa
     2401 gtgcatgtga ccgatgatgc tcagaactat catggcgtcg ccgggcgctc agatgtgggg
     2461 cagccaaaga atttcgtcgt tgacaatacg ccgtcagaca tccttggctt ccagtttctg
     2521 ctgaatccaa accatccgtt tgcctgggtc gggaatgtcg gttctgcacc cgtcctgttg
     2581 accggacagt atgacaactt gcgggttgat ggtctgggcg cgtcgattac cgtgaactcc
     2641 gggtgtcggc aggcgtgggt agacctgacc aacatccagg caacgtcaac cttcatgctc
     2701 atcaatacga cggtcgccgg cggacaaaca actggtacaa ctccgcccgc gggttatgtc
     2761 aaactcgcga cggtgagtct cgagctggag ccggctctgg gcccggggac ctactcgttc
     2821 aacatcaccg tggtcgatac agctggtaac cgcatggcct cctcggccga acaggcgttc
     2881 gacacgacgc gaccatattt gatgcgtgcg gaatacgacg gcagttacct gaccctgcag
     2941 tttaacgagc cgctgaaagt cggcgattac acgctgacga tgcctgagcg cttcgaccag
     3001 accaagattc gcatcggcaa taaaagtaat ctggaactgg tgggtccgga ttcggttgta
     3061 gctctttccg ctctcgatct ggttcaagat ccctatgccg atactgacac gatccgcatt
     3121 gcgctggctt cggggactcg agcgacgatc gccgactggg ggaacgtctc gatctggatg
     3181 tctatgggtg ccaccggacc tacgggtgaa ggcgcctcag tctcagtcga cctggcgagc
     3241 aactgggtga atccgatcaa gcagagtgcg ggtcgggtca tcaacgtgcc cgttccgtat
     3301 gcgatcaggc cgaagatcat cggtggtgcc tatgatgcgg tcaacgtgcc ttcgattctc
     3361 tacatcgact ttgacaaacc gatggcgacc gacaccatga ccaacttcac gctgccagcc
     3421 tttgcggtgg ttcgagagcg ccgaaacgac ttgcgcagtg tgtactatcc gtggcgatac
     3481 aatccggtca ttggttcaga cacggttggt gtgccatcgt cgacgacgcg tttggccatc
     3541 aatctgtctc ctgaagctca ggactggaac gccattaaat tcggccgcct ggccagtcaa
     3601 ttgaattatg cggtctcgac ggcaacgcct ccgctcgtcc gcgatgaaaa agggaatggc
     3661 gtccagccga tctcctttga caacgcggta gccgcagcgt tgtctccgga gaatcgttca
     3721 tttgaagtcg aaccgacact tggcaacgag atcgttctgc atcttgcctc caagacgttg
     3781 acgattcctc tgaatcgccg ggctctgatc tggggtggtg agtttgccga caacacaccg
     3841 aagttcaacc ccgatttcaa cagactgaat gtcattccat cacgggtgtt cttgtacaaa
     3901 cgagctgata tgactggctc gagcatctca ctgaatcggg cagctaacaa tttgcccgca
     3961 tggccgattt acaacacctc ctttgcctcg acgaccgtca tcattcccct cagtgatgac
     4021 gacatccgcg acgttctctc ctggaattca ggaagtctgt acgtggccat gaacgaaggg
     4081 gctttcaaag atctgtgggg caacaacagc agccgatatc cgagctcaac caccacggcc
     4141 gcgccggtaa cggtggtgaa gccggttggg tatccgggtc cgcaagtgat ctcggtcgcg
     4201 gtcagtgatc cgacccccac c
//
LOCUS       DAORHD010000270         4205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22717_length_4205_cov_3.739036, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000270 DAORHD010000000
VERSION     DAORHD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22717_length_4205_cov_3.739036"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4205)
                     /locus_tag="PKO06_07030"
     CDS             complement(<1..>4205)
                     /locus_tag="PKO06_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69429.1"
                     /translation="RQLFRDWVRHLPRTIVQLLTFSSFIDIMQIATDDPMRIGHAISG
                     LTSGGGTEVVKAVDAALRGLQGIPSQRRFLLFVTDAAIQVAPTDQVKFEALLGAFKDA
                     GIRSLWVGMVPSESPAIRDTFTRVSRLAGGEFIISPGVQALQEALDRVHRLAMNPASE
                     TAAIPLQILVDEPQPDLSHTVIEGNSLFPFPPSARQRPLSVGCLQVSTRSPTVPQSTL
                     SPTGTAPGAKGSKARTETVEQNRLSIGASGETTAVRLTVTDATILSRLQGIDAGSNRR
                     FVSLRLTLENRLPEQEVTILDGGEMHPSAWVKGEARGKTVRTVPPYLIPDLHRHLFLR
                     WNDGIEVPVSPASVFLPDPLYNPELPSIMIASGSQVSGQVVFLVDTPHLQTASLHLYD
                     TAWGHLDLPLVGSMTARPRPLSELPTRATGKLSDTFALAVQAVTDQTDAVAGVAPAAD
                     MRWRTIQLSLESKVQAHLAFDPTARLRLVAETPRGPVVLRLSPLTDRIPGGWSSSSLF
                     MPGSRNLVSLVFPFPSGLASAPSSIHADLRKSDLLLPLVGSEPVAIMSEPPAALTQTN
                     GTASGTAFVATDSAWQSLGLSVTVNQATVCGKSAGFSLDRLVVDLTTSDRRDGYATRL
                     WNLFELLREDQTERDATGQLVRRRFQSRKVKVTENASERKGLGSFTKNEARSQITKLA
                     PLAMTGDYLFGIRPDTSVADGTRQRNLLLFDVPTEGNWYLAIGDHVLPEPVRPADGPT
                     IEPWFLAPQPKIPSEQSGSIEQQVAKAIQELSRRGELKRLAAARGEATPRVIDDTGAS
                     VRLEAPPLSLSWAGEKRWLSLLQGSEDQLWETLRQIRLDPGTDKPGAMVGPEALLTQQ
                     HGTVEDMRQAVTVWYAERRLPATLKRVALSESGKTEADRLMDLELATTTAAADSSALP
                     AYVCEAGTRTWVLPFCRPIDETASWFEPDTAKRAADELVECDIAVNVVCEPANADQAA
                     AMGDMGSALAGGSGRSAKSFQMFKGRFSCCDLSRDALDLYYTESRLGRTRSLSAIVLG
                     PTGRQEGTTAIDVSSWRPVREEIEVRVRGCPRIDTYVRRLEDEPLTESFHSVAIAAPD
                     ISASAALELSAAWQTSKPATLPDAVSILRWASRARLAGFLAAQARYERQSAIALGVRL
                     TRQQHPRVLIYTGMRQGDTWTERLDLRQHVPAVTGPEEAVKAFLLMHGLTSTACEGAG
                     IGGKSVIDVWKSTGDYLVVPAKSRNAFLRELKARAFPEDLQKMLKDGNGVLLFSRRPQ
                     IQAGKPVWGWLEIDPKTFVTQSTFSTGDRGGMVEEAIMENMVDGNSYAIGFIVGIDAS
                     IWSVAAFSLEESRYEEILARGETFANSLASCFNGIGEEPSFAPEDGPVSFGLDGVSYN
                     DGEDSSYRSFTDGYNAGVKYYFAR"
BASE COUNT          815 a         1241 c         1350 g          799 t
ORIGIN      
        1 cgtgcgaagt agtacttgac cccggcgttg tatccatcgg taaagctgcg ataactcgag
       61 tcttcgccgt cgttgtaact gaccccgtca agtccgaacg agaccgggcc gtcttcggga
      121 gcaaacgaag gctcttcgcc gatcccgttg aagcacgaag ccaggctgtt cgcgaatgtc
      181 tccccgcgcg ccagaatttc ctcgtagcgt gactcttcaa gggagaaggc ggcgacgctc
      241 cagatcgagg cgtcgatgcc gacgataaag ccgatcgcat agctgttgcc gtcgaccatg
      301 ttttccatga tcgcttcttc caccattccg cctcggtcgc cggtcgaaaa cgtcgactgg
      361 gtgacgaagg tcttcgggtc gatctcgagc cagccccaca ccggtttgcc ggcctgaatc
      421 tgcgggcgac gtgagaagag cagcaccccg ttcccgtctt tcaacatttt ctgcaggtct
      481 tcaggaaacg ctctggcctt gagctcgcgc agaaacgcgt tccgtgactt ggccggcacc
      541 acgagataat cgcccgtgga cttccacaca tcgatcacgg acttgccgcc aatgcctgcc
      601 ccttcgcagg cggtcgaggt cagaccgtgc atgagcagaa aggctttcac ggcttcttcc
      661 gggccggtca cggcgggaac atgctgtcgc aggtcgaggc gctctgtcca ggtgtcgccc
      721 tgacgcatgc cggtgtagat caacactcgc gggtgctgct gccgggtcag gcgcacgccg
      781 agagcgatgg ctgactgccg ttcatatcgt gcctgcgcgg cgaggaagcc agccagacgc
      841 gcccgcgacg cccagcgtaa aatcgaaacc gcatccggca acgttgcggg cttgctggtc
      901 tgccaggcgg cggacagctc aagagcggca gaggccgaga tatccggtgc cgcgatggcc
      961 accgagtgaa aagactctgt cagaggttcg tcttcgagcc ggcgaacgta ggtgtcaatg
     1021 cgcgggcatc cgcgtacccg cacctcgatc tcttcgcgaa caggtcgcca cgagctgaca
     1081 tcaatcgctg tcgtgccctc ctgtcgcccg gtcgggccga gcacgattgc cgacagactt
     1141 cgggttcgtc cgagtcgaga ctcggtgtaa taaagatcaa gagcatctcg agatagatcg
     1201 caacaggaga accgcccttt gaacatctga aaggattttg ctgatcgtcc cgatccgcca
     1261 gcaagggcac ttcccatgtc gcccatcgcg gcggcctgat ccgcattggc tggttcgcag
     1321 acgacattca ccgcaatgtc gcactcgacc agctcgtcgg cggcacgttt ggccgtatct
     1381 ggctcgaacc aggatgcggt ctcgtcgatc gggcggcaga agggcagcac ccaggtgcga
     1441 gtacctgcct cgcacacata cgccggcagg gcgctggaat ctgcggcggc ggttgtggtc
     1501 gccagttcga gatccatcag ccggtccgct tccgtttttc cggattcaga aagggcaacg
     1561 cgtttcaacg tcgctggcag tcggcgctcg gcataccaca ccgtgactgc ctgcctcatg
     1621 tcttcgacgg tcccgtgctg ctgggtcagc agcgcttcag ggccgaccat cgcgcccggt
     1681 ttatcggtgc ccgggtcgag tctgatctgc cgaagggttt cccacagctg atcttccgaa
     1741 ccctgcaaca ggcttagcca gcgcttttct cccgcccagg acaacgacaa agggggagct
     1801 tcgagtcgta ccgacgcgcc ggtatcgtcg atgacgcgcg gtgtggcttc gccacgggcg
     1861 gctgcgagcc gcttgagttc tccgcgacga ctcagctcct gaatcgcttt tgcgacctgc
     1921 tgctcgatgc tgccgctttg ttctgatgga atcttcggtt gcggtgccag aaaccagggt
     1981 tcaatcgttg gtccgtcagc ggggcgcacc ggttcaggta atacgtggtc tccgatcgcc
     2041 aggtaccagt tgccctcggt cggaacgtcg aagagaagga ggtttcgctg tctggttccg
     2101 tcggcgacgc tggtgtcagg gcgaatgccg aacagatagt cgccggtcat cgccaggggt
     2161 gccagtttgg tgatctgaga acgcgcttcg tttttcgtga agctgccgag gccctttcgc
     2221 tcagatgcat tctcggtgac tttgactttg cggctctgga aacggcgacg aacaagctga
     2281 ccggtcgcgt cacgctctgt ctggtcttcg cgcaacaact caaacagatt ccacaggcgg
     2341 gttgcgtagc cgtcgcggcg gtcgctggtg gtcaggtcga cgacaagacg gtcgagtgaa
     2401 aaaccggcgg acttgccgca gacagtggcc tggttgaccg ttacggacaa cccgagcgac
     2461 tgccacgccg aatcggtggc aacgaacgca gtgccgctcg cggttccatt cgtctgcgtg
     2521 agagccgcgg gaggctcgga catgatggcc acgggctctg aaccgacgag cggtaacaga
     2581 agatctgact tgcgcagatc ggcatgaata gacgatggcg ctgaagcgag cccggacggg
     2641 aacggaaaca cgagactgac gagatttcgc gagccgggca tgaacagact cgaactcgac
     2701 catcccccgg ggatgcgatc ggtcagcgga gaaagtctca gcaccaccgg accacgaggc
     2761 gtctcggcaa cgaggcgcag gcgagcggtt ggatcaaacg ccagatgcgc ctgcactttc
     2821 gattccagag agagttgaat agtacgccag cgcatgtcgg cagccggggc gacaccggcc
     2881 accgcgtcgg tctgatcagt gaccgcctgc acggcgagag cgaaagtgtc ggaaagtttg
     2941 ccggtggcgc gtgtcggcag ctcagacaga ggtctcggac gcgccgtcat cgagccgacc
     3001 agcggcagat cgagatgacc ccaggccgtg tcatacagat gcagtgacgc ggtctgcaga
     3061 tggggcgtgt cgaccagaaa gaccacttgt cctgacactt gtgaacctga agcaatcata
     3121 atgcttggca attctgggtt gtacaatggg tctggcagaa agacggaggc gggagagacg
     3181 gggacttcga tgccgtcgtt ccaccgcaaa aacaaatggc ggtgaagatc gggaatcaga
     3241 tagggaggaa ccgtccggac ggttttgcct cgcgcttcgc ctttgaccca cgctgaaggg
     3301 tgcatctcgc cgccgtcgag aatggtgact tcctgctcag gaagacggtt ttccagggtg
     3361 aggcgcagcg acacgaaccg gcgattcgaa ccggcatcga ttccctgcag cctggagagg
     3421 attgtggcgt cggtgacggt cagacgcacg gctgtcgttt caccggaagc gccgatggac
     3481 agacggttct gctcgacggt ctcggtgcgg gctttcgaac ctttggctcc gggagcggtt
     3541 ccagttggtg acagggttga ctgtggaacg gtcgggcttc gggtggatac ctgcagacaa
     3601 ccgacgctca gcggtcgctg acgcgcggaa ggggggaagg ggaacaggct gtttccttcg
     3661 atgacggtgt gcgacaggtc gggttgaggt tcgtcaacca gaatctgcag cggtatcgcc
     3721 gccgtctcgc tcgcggggtt catcgccagg cggtgtacgc gatccagtgc ttcctgtaga
     3781 gcttgaactc cagggctgat gatgaattcg ccgccagcca acctggacac tcgggtaaac
     3841 gtgtcgcgaa tcgccggtga ctcactcggc accatgccga cccagagcga ccgaatgccg
     3901 gcgtctttga acgcgccgag cagggcttcg aacttcacct ggtcggtggg agcaacctga
     3961 atggcggcgt cggtaacgaa cagcagaaag cgtcgttgcg agggaatgcc ctggaggccg
     4021 cgcagggccg catcgaccgc cttgaccacc tcggtgccgc ccccggaggt cagcccgctg
     4081 atggcgtggc cgatgcgcat cggatcgtcg gtggcaatct gcatgatgtc gatgaatgaa
     4141 gaaaacgtca gcagttgcac gatggtgcga ggcaggtgcc tgacccagtc gcgaaagagc
     4201 tggcg
//
LOCUS       DAORHD010000271         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22815_length_4193_cov_3.562832, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000271 DAORHD010000000
VERSION     DAORHD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22815_length_4193_cov_3.562832"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1933)
                     /locus_tag="PKO06_07035"
     CDS             complement(<1..1933)
                     /locus_tag="PKO06_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69430.1"
                     /translation="MIEEILISLQDKLRSADYLDCETAVDKLGSLLQSQNAEKQRKII
                     ATTLPLALSSKFESIQKKAVQVACNYPSVLRDILPALKLSTDTTVRFWAFFMLIELNF
                     LTQTELHEIADSKENDEIRKFVLEALARKPDKAVILCLLEKISDPSWIIRKQAKKLLI
                     EQGDSIYQTIQEFFMTCPSRQKYDCIKIIPLILKDKASTLFQRMLEADRNGVVRPYIC
                     AGLGEIRSEAAMSTLISLLADPSGVVREEAIKALTNWGRDVVRPLVAIFPTASQDTRT
                     SIMVLLGRVLGLDVVKEAEEFFGEATQESKYYLLTALGEVREPLVVKELLPLLKDESV
                     FIQEHTIRIMARLGVHALDPLLASLDTDDEKLILPVLKVIGEIGSKDALRPLLFIIDN
                     SKNTLIRTCAIEAIAKLQKFELIAGLLLLKLDDRDITIRHTIVENLSRHQRQAFIKDL
                     MMASMDRNSEVSYWSKEILKRRDYPGIPCFLMMYEQATDYEKEKVLSLTSKLTNEQID
                     AVLRKERVTIESLQPQNVETRVMVRKYSKENITDIKELLYYLHEEGGSDLHISIGLPP
                     SIRHHGDLVRTTFETITPEKSRYLLYSLLNDAQKEIFHQKMEIDLSFEIPDCARFRAN
                     IFNQKNGVAALASAQRGLQV"
     gene            complement(2116..2850)
                     /locus_tag="PKO06_07040"
     CDS             complement(2116..2850)
                     /locus_tag="PKO06_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV69431.1"
                     /translation="MKTGKTPGETTSEARLLTVREAARLLRLSPHTLQSWMSPSSPNY
                     RPEFSRLALRAGRRTLYRTTDLLTWLEQHQATRSILPGSERSVNWFERFVAARGTLNL
                     PYQSEPTPDPSPDSLFRGGFLALDGAPLFAWLANTSEAPRILRLARRAEGLVLPLPTA
                     GWMLRRLRRFPNALDQIQSLLFRSGIFEAASLNEEAVSRISQLPSAIADVAAMSYGAA
                     IVHGASVFATWNTALLEAPGALVLAP"
     gene            3148..3963
                     /gene="rpsB"
                     /locus_tag="PKO06_07045"
     CDS             3148..3963
                     /gene="rpsB"
                     /locus_tag="PKO06_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNV69432.1"
                     /translation="MALVSMKALLEAGVHFGHQTRRWNPKMAKYIFTSRNGIYIIDLQ
                     KTTKLLDDACEFVKSVVRQGRPILFVSTKRQAQDIIKAEAERCGMYFVNYRWLGGMLT
                     NYITIEKRIQRLRDLDDLIESGKIESYPKKEAAGLRKEREKLNRALGGIKNMKGMPGA
                     LFIIDPHKEAIAVAEARKVGVPIVAVVDTNCDPDQIDYVIPGNDDAIRAVKLVTSLIA
                     DAVMESLEGRTFNEAQAVASDQAVMAEAAGVAPAAVAAKAPEQEESAEKLVIG"
     gene            4112..>4193
                     /gene="tsf"
                     /locus_tag="PKO06_07050"
     CDS             4112..>4193
                     /gene="tsf"
                     /locus_tag="PKO06_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531438.1"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HNV69433.1"
                     /translation="MEITAKMVSELREKTGAGMMECKEALK"
BASE COUNT          948 a         1048 c         1245 g          952 t
ORIGIN      
        1 cgacctgcag gccgcgctgc gcactcgcca gcgccgcgac gccgttcttc tggttgaaga
       61 tattcgcgcg aaaacgagcg caatccggaa tttcgaacga aaggtcgatt tccatcttct
      121 gatggaaaat ctctttctgg gcatcgttca gcaatgaata cagcaggtag cggctttttt
      181 caggggtgat ggtttcgaac gtggttcgca ccagatcgcc gtgatgacgg atgctgggcg
      241 gcaggccgat ggaaatgtga agatctgagc ctccttcttc gtgcaggtag tacaacagct
      301 ctttgatgtc cgtgatattt tctttgctgt acttgcgcac catgacgcgg gtttccacat
      361 tctggggttg cagactctcg atcgtgactc gttctttgcg cagaacggca tcgatctgtt
      421 cattggtcag tttcgacgtc agcgaaagaa ccttctcttt ttcgtaatcc gtggcctgtt
      481 catacatcat cagaaaacag ggaatacccg gataatcgcg tcgtttcaat atttccttgg
      541 accagtagga cacttcggag ttgcgatcca tgctcgccat catgagatct ttgatgaacg
      601 cctgacgctg atggcgtgac aggttttcga caatggtgtg gcgaatcgtg atgtcgcggt
      661 catcgagctt gagcaacaac aggccggcaa tgagctcaaa cttctgcagt ttggcaatcg
      721 cttcgatggc acaggtgcga atgagggtgt tttttgagtt gtcgatgatg aaaagaagtg
      781 gtcttaaagc gtcttttgaa ccgatttcac caatgacttt caataccgga agaatcagtt
      841 tctcgtcatc ggtgtcgagt gaggccagca agggatcaag ggcgtgaaca ccaagtctgg
      901 ccatgatccg tatcgtatgt tcctgaatga aaacggattc gtctttcagg agcggcagca
      961 gttctttgac cacgagcggt tctcggactt cgcccagggc ggtcagcagg taatacttgc
     1021 tttcctgggt ggcttccccg aaaaactctt cggcttcctt caccacatcg agaccaagaa
     1081 ctcgaccgag cagcaccatg atcgaggtgc gggtatcctg gctggcggtc gggaaaatgg
     1141 ccacgagagg ccgcaccacg tcacgacccc agttggtcag cgctttgatc gcttcttcgc
     1201 gcacgactcc cgagggatct gcgagcagtg aaatgagagt cgacattgcc gcttcggagc
     1261 ggatttctcc gagaccggca cagatgtacg gccgcaccac accgttgcgg tcggcttcga
     1321 gcatgcgctg aaacagcgtc gacgccttat ccttcaagat caagggaatg atcttgatgc
     1381 aatcatattt ctgccgactc gggcaggtca tgaaaaattc ctgaatggtc tggtagatcg
     1441 aatcgccttg ttcgatcagc agcttcttgg cttgtttgcg gatgatccag ctggggtcgc
     1501 tgattttttc gagcaggcag agaatgacgg ctttgtccgg tttgcgcgcc aaagcttcga
     1561 gaacgaactt gcgaatctcg tcattttctt tgctgtcggc gatctcgtgc agttcggtct
     1621 gcgtgaggaa gttcagctcg atgagcatga agaaagccca gaagcgaacc gtggtatcgg
     1681 tcgagagctt gagggccggc agaatatcgc ggagcacgct tggataattg caggcgacct
     1741 gaaccgcttt cttctggatc gattcaaact tgctggagag agcgagtgga agcgtcgtcg
     1801 caatgatctt gcgctgcttt tcggcgttct gtgattgcag cagtgaaccg agtttgtcga
     1861 cggccgtttc gcaatcgagg tagtcagcgc tgcgtagttt gtcctgtaag ctgatcagga
     1921 tttcttcgat catggtcgct ccctggcccc catagtatga ctatggaatt atatcaggag
     1981 ttcacggaaa gtccaggaaa accgaattca tccaggctgc attcacgatc ccgggttact
     2041 ccggaaacgt tcgttcgcgc catctgtgcg agggtggatg gaggtgattc gtcgggtaca
     2101 ccggcggcaa gtcagtcaag gagccaggac gagggcgccg ggggcctcca gcagcgcggt
     2161 gttccaggtg gcgaacacgc tcgctccgtg aacgatcgct gccccgtagc tcatggccgc
     2221 aacatcagcg atcgcggaag ggagttggga gatgcgggat acagcttctt cattcaggct
     2281 ggcggcctcg aaaatccctg agcggaagag caggctctgg atctgatcga gtgcattggg
     2341 aaagcgtctc aggcggcgga gcatccagcc agcggtgggg aggggaagga cgagtccttc
     2401 cgcgcgtctg gccagtcgca gaatgcgtgg ggcttcgctg gtgtttgcca gccaggcgaa
     2461 caggggagcg ccatccagag ccagaaagcc accgcgaaac aagctgtctg gtgaggggtc
     2521 tggtgtcggc tcgctttgat aggggagatt gagtgtgccg cgggccgcga cgaaccgttc
     2581 gaaccagttg accgaacgct cgctgccggg cagaatcgat ctggtggcct gatgttgttc
     2641 aagccaggtc agaaggtctg tcgtgcggta cagagttctc cggccggctc tcagtgccag
     2701 tcgcgaaaac tctggccggt agttgggcga tgagggagac atccaggact ggagcgtgtg
     2761 gggtgacagc cgcaacagtc gagccgcttc tcgtaccgtc aggagtcgtg cctcggatgt
     2821 tgtctcgccg ggcgtttttc ctgttttcat tgtatataac agtatataac aatatataac
     2881 aatatgcaag atgattgaat ccgcaattga tcatgaagag aagacctgtt atcaggaaaa
     2941 actgctgtaa tagcgctctg ccgagatcga ggccggtgca ataggcttcg aaagcgagaa
     3001 aacggaaggc agaggaagtg aggacggccc gaaaacgctg tcgagaagag aaaagtactt
     3061 gaccgtggtc tggagttggc ttacaatagg atctcttcct gcgataacgc cgggattttg
     3121 aatggacatc atctggaggg atagacaatg gctctggttt ctatgaaagc gttgcttgaa
     3181 gcgggtgtac actttggcca tcagactcgc cggtggaatc ccaaaatggc gaagtacatc
     3241 ttcaccagcc gcaatggcat ttatatcatc gatctgcaga agacgacgaa actgctcgat
     3301 gacgcctgcg agtttgtgaa gagcgtcgtg cgtcagggtc gtccgattct gttcgtttcg
     3361 acgaagcgac aggcgcagga catcatcaaa gccgaagcgg aacgctgcgg catgtatttc
     3421 gtgaattatc gctggttggg cggcatgctg accaactaca tcacgatcga gaagcgcatt
     3481 cagcgcctcc gcgacctcga tgatctgatc gagagcggca agatcgaatc gtaccccaag
     3541 aaagaagcgg ctggtctgcg caaagaacgc gaaaagctga atcgcgctct gggcggcatc
     3601 aagaacatga agggcatgcc tggcgctctg ttcatcattg acccgcacaa agaagccatt
     3661 gcggtggccg aagcccgcaa ggttggggtt cccattgtgg ccgtggtcga cacgaactgc
     3721 gaccccgatc agatcgacta cgtcattccg ggcaacgacg atgcgattcg cgcggtgaag
     3781 ctggtaacca gcctcattgc tgatgccgtc atggaatcgc tcgaaggccg caccttcaac
     3841 gaggctcagg ctgttgccag cgatcaggct gtcatggctg aagccgctgg cgtcgctcct
     3901 gccgcggtgg ccgcaaaagc ccccgaacag gaagaatcag ccgaaaagct ggtcatcgga
     3961 taattactca tccggcgagg gcgcggccct cgccgggttt gttgcctttg gtatttttac
     4021 aaagtcaaca acatctaccg cactcgacgt acgatcggtc cgttttcccg ggccgaatct
     4081 ctgagaaatc tgcaacagaa gggtaatcga aatggaaatt actgcgaaaa tggtcagtga
     4141 acttcgtgag aaaaccggcg ccggcatgat ggaatgcaag gaagccttga agg
//
LOCUS       DAORHD010000272         4193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22818_length_4193_cov_3.383277, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000272 DAORHD010000000
VERSION     DAORHD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22818_length_4193_cov_3.383277"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2780
                     /locus_tag="PKO06_07055"
     CDS             <1..2780
                     /locus_tag="PKO06_07055"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNV69434.1"
                     /translation="LQVDGLPLTRLGVPPQEARGEVSLIGMLSGSLKQPGFAGDYALE
                     NAAWQGLEFESLRGHLKLAQAHLTFAPLLAKLAGGGTVDGGFEFDLKKERLLGLRLGF
                     NNLALDRARVLMPPDLAQLEPTGFVSGTVQRQNEGADFWWDFRVAGDRVLLASETLHR
                     FNLEGTVSSDTVEVRSLDAGIWGGQVEAHGRVDRLQAELELSARDLEIQKSHQVQRLA
                     SPSAGLISLQGNIEFGRRRRHGYFTLFGKDLHVRGQTLGNLGAEVAIDAEGLKIVRAD
                     LDRIGMQLDGSVSWDAGNPYQWKLQLKQTDLSFIPSSSGWLEVQPGDVIVTGHMEMAG
                     HWNEKRPREALARFESIEIHRGTDLIVANRPVDVRFQNEAFEIRSLELKYRNGVFGVS
                     GIWNPHGNAALTLSGQDFSVAALARLAGIHTEVPEGRLSIAGGISGVYPALEMKGDVT
                     VQDLEFRGRKVPFLKAKCRVDPVGMAVNPLVAEFPRNKITAVGSLPFEQDGGVGSLDL
                     QVEVASGPLDDLPVYLPEFFNTAKGWGKASLHFSGNPLYPQITGVARVHGDSIGFKGM
                     KRPLTQVEIGVNTDKGLVRIEPMQAKLGRGVFSGFGEVDFRDGPGSLTVKLSGQKLDV
                     SWGRIDIERASASVDVSGNLYNPVVAGHVQIPKGKVQIADDLLGKSSFNLNLPLESLR
                     YTVYVDIPRNLWLKNSMINAETRGKFKLEGDLNDFQLDGGISTVQGWLTFQRRKFTID
                     NGEIKFGEQDRKFDPHIYFKSVTNIQNTQVFLVLDGRLSSIKPQLYSSPPQSEGTLLA
                     MLTLGRSMDEVMMGTGDRISFEKEILDGLKNTYLSGLLSSTLSEAFNLDELYFDSLFD
                     RTSGVTRSFLRIGKYIGYNIFLAYEGTLSNDGMKTYIIEYRLPRGFMLNVEVEKPRNN
                     TRIGIKYDWKF"
     gene            2796..3758
                     /locus_tag="PKO06_07060"
     CDS             2796..3758
                     /locus_tag="PKO06_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV69435.1"
                     /translation="MTDIRNFFLQLETSLGSGIAITRALQLISKNLSGSLRRKVEAMG
                     QAIEHGSTFSDAMARAGRPFEKMHISFIRFGEQSGNLDKVCGALARHAEKEVAVSRKV
                     INSMLYPGFVLLFAICMGPILEVILAGKPYSDGFPGAAMNAGIFAVVVGGFYGLYASG
                     VFSFLDAIVIHLPFLGGILEKLALARFTRALAVGESAGVPLVEALNTAIDVSANGYIQ
                     KQLSHLPRHVGGGKGISSGLEHVTCLPGTLKEMIMVGEQSGKLSEMLEKTAVYFEEEA
                     NNRIDGLMKVLPALFFLAVALYVGSMIIPIAKNVFEGGMGALNK"
     gene            3791..>4193
                     /locus_tag="PKO06_07065"
     CDS             3791..>4193
                     /locus_tag="PKO06_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69436.1"
                     /translation="MKEWIERLFNVRSGEWGDLLFFWVLNTLLCTGLALGEALAETLF
                     LKRIGVDFLPFMFILCSLVAIPISLLLTHLQGSVRQTTLTMGIHGVALIMVAFSAYLV
                     QSNTLFFGRAIGTPVFYLAEVLLTIVLSTHFS"
BASE COUNT          803 a         1142 c         1315 g          933 t
ORIGIN      
        1 agttgcaggt cgatggcctc ccgctgaccc gtctcggagt gccgccacag gaagcacgcg
       61 gcgaagtctc gctgatcgga atgttgtcgg gttccttgaa acagcccggg tttgccggcg
      121 attatgcgct cgaaaacgcc gcctggcagg ggctggagtt cgagtctctg cgcggacatc
      181 tgaagctcgc gcaggcccac ctgaccttcg ctccgctgct ggcgaagctg gccgggggag
      241 gcacggtcga cggcggcttc gaattcgatc tgaagaaaga acgcctgctt ggtctgcgcc
      301 tgggtttcaa caacctggca cttgatcggg ctcgagttct gatgcctcct gatctggcgc
      361 agctcgagcc gaccggtttc gtatctggta ccgttcagcg tcagaacgag ggcgcggatt
      421 tctggtggga cttccgtgtc gccggtgacc gcgtgttgct ggcttctgag acgctgcatc
      481 ggttcaacct cgagggaacg gtttcatcag acaccgtgga ggtgcgctcg ctggatgccg
      541 ggatctgggg agggcaggtg gaagcccacg gacgcgtcga ccgcctgcag gccgagctgg
      601 aactctcggc ccgcgatctc gagattcaga agtcgcacca ggtccagcgt ctggcgagcc
      661 ccagtgccgg tctgatttcg ctgcagggca acatcgagtt tggcaggcgc cgcagacacg
      721 gctacttcac gctcttcggc aaagatctcc acgtgcgggg ccagactctg ggcaatcttg
      781 gcgcggaagt ggcgatcgat gccgaggggt tgaaaatcgt gcgtgccgat ctcgatcgca
      841 ttggcatgca gctcgatggg tccgtaagct gggatgcggg gaacccgtat cagtggaaac
      901 tgcagttgaa gcagaccgac ctctcgttca ttccgtcctc gagcggttgg cttgaggtgc
      961 agccgggcga tgtcattgtc acggggcaca tggaaatggc cgggcactgg aatgagaagc
     1021 gaccgcgcga agctctggcc cgttttgaat ccatcgaaat ccatcgtggc accgatctga
     1081 tcgtcgcgaa tcgcccggtc gatgttcgct ttcagaacga ggctttcgag attcgttcgc
     1141 tcgaactcaa gtatcgcaat ggggtgtttg gcgtatctgg catctggaac ccgcacggaa
     1201 acgccgcctt gacgctgagc ggccaggact tttccgtggc ggctctggcg cggttggcgg
     1261 gaattcatac cgaagttccg gaaggacgtc tgtcgattgc gggcggcatc agtggtgtgt
     1321 atcccgctct cgagatgaag ggtgatgtca ccgttcagga tctcgagttt cgcggcagaa
     1381 aggtgccgtt tctcaaggcg aagtgccgcg ttgatccggt cggcatggcg gtgaacccgc
     1441 tggtggccga gttcccgcgc aacaagatta cagccgtcgg ctcgttgccg ttcgaacagg
     1501 atggtggcgt gggttcgctc gacctgcagg tcgaggtggc ttcaggaccg ctcgacgatc
     1561 tgccggtcta tctgcccgag tttttcaaca cggccaaagg gtggggcaag gcgtcgctgc
     1621 atttttccgg taacccgctc tatccgcaga ttactggagt ggcgcgggtg catggcgatt
     1681 cgatcgggtt caagggcatg aaacgtccgc tgacgcaggt cgagatcggc gtgaacaccg
     1741 acaaaggcct ggtgcgcatc gagccgatgc aggccaagct cgggcgcggc gtgttttcgg
     1801 gattcggtga ggttgatttc cgtgacggtc cgggatcgct gaccgtgaag ctgtcgggcc
     1861 agaagctcga cgtgtcctgg ggtcgcatcg acatagaacg tgcttcggcg agcgttgacg
     1921 tcagcggcaa cttgtacaat ccggtagtgg caggtcacgt gcagattccg aaggggaagg
     1981 tgcagatcgc tgatgacctg ctgggcaaaa gcagtttcaa tctgaatctc ccgctcgagt
     2041 cactgcgcta cacggtgtat gtcgatattc cgcgcaacct gtggctgaag aactcgatga
     2101 tcaatgccga gacccgcggc aagttcaaac tggaagggga tctgaacgat tttcagctcg
     2161 atggcgggat cagcacggtg cagggctggc tgacctttca acggcgcaag ttcaccatcg
     2221 acaacggcga gatcaagttc ggcgaacaag accggaagtt cgatccgcac atctacttca
     2281 aatcggtgac caacattcag aacacccagg tgtttctcgt gctcgacggg cgtttgtcat
     2341 cgatcaagcc gcagttgtac tcgtcgcccc cgcaatcgga aggcacgctg ctcgccatgt
     2401 tgacgctcgg tcgcagtatg gacgaggtca tgatgggcac cggtgaccgc atttcgttcg
     2461 aaaaggaaat tctcgacggt ctgaaaaaca cgtatctctc aggcctgctc agttcaacgc
     2521 tttcagaggc attcaacctg gacgaactct attttgattc gctgttcgac cgcaccagcg
     2581 gcgtgacgcg gtcgtttctg cgcatcggca agtatatcgg ttacaacatc tttctggcct
     2641 acgaaggcac cctgtcgaac gacggcatga agacgtacat cattgaatac cggctgccgc
     2701 gcggcttcat gctgaacgtt gaagtcgaaa aaccccgcaa taacacgcga attgggataa
     2761 aatacgattg gaaattctaa ccgagaggga agatcatgac ggatattcgt aatttctttc
     2821 tgcagcttga gacttcgctg ggttccggaa tcgccatcac gcgcgcgctg cagctgatca
     2881 gcaagaatct gagtggctcg ttgcgtcgca aggttgaggc gatgggccag gccatcgagc
     2941 atggctcgac gttttccgat gccatggccc gcgccggtcg cccattcgag aagatgcaca
     3001 tcagcttcat tcgcttcgga gagcagtcgg gaaatctcga caaggtctgc ggagctctgg
     3061 cgcgtcatgc cgagaaggaa gtggccgtca gtcgcaaggt catcaactcg atgctgtatc
     3121 cgggtttcgt gctgttgttt gccatctgca tgggcccgat tctcgaagtg attctcgcgg
     3181 gaaaacccta ctcggacggc tttcctggag cggcgatgaa tgcgggcatc tttgcggtcg
     3241 tagtgggggg attctacggc ctgtatgcct cgggggtctt ttcgtttctc gacgcgatcg
     3301 tcattcacct gccattcctg ggcggaatcc tcgaaaagtt ggctctggca cgctttacgc
     3361 gagcgctggc cgtgggcgaa tccgcgggtg ttcccctcgt tgaagccctc aacacggcga
     3421 tcgacgtgtc agccaacggg tacattcaga aacagctgtc gcatctgccg cggcatgtcg
     3481 gtgggggcaa gggcatttcc agcggcctcg agcacgtgac ctgtctgccg ggcaccttga
     3541 aagagatgat catggtcggc gagcagagcg gcaagctctc ggaaatgctc gaaaagaccg
     3601 ctgtctactt cgaggaagaa gccaacaatc gcatcgatgg tctcatgaag gtgctgccgg
     3661 cgctgttctt tctcgccgtc gccctgtatg tcggttcgat gatcattccg attgcgaaga
     3721 acgtttttga aggcggcatg ggggccctca acaagtagga cgatttcgcg aaaggcggag
     3781 cgggtaagtc atgaaagaat ggatcgaacg cctgttcaac gttcgctcgg gcgaatgggg
     3841 cgatctgctg ttcttctggg tgctcaatac cctgctctgc accgggctgg ccttgggcga
     3901 agcgcttgct gagacgctct ttttgaagcg catcggcgtc gattttctgc ccttcatgtt
     3961 cattctctgt tcgctcgtcg ccattccgat ctcgctgctg ctgacgcatc tgcagggatc
     4021 ggtgcgccag acgacgctga ccatgggaat tcacggtgtc gctctgatca tggtggcatt
     4081 ctcggcctat ctcgtgcaga gtaacacgct gtttttcggt cgggcgattg ggactcccgt
     4141 gttctatctg gccgaggtgc tgttgacgat cgtgctcagt acgcacttct cgg
//
LOCUS       DAORHD010000273         4190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22833_length_4190_cov_3.840145, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000273 DAORHD010000000
VERSION     DAORHD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22833_length_4190_cov_3.840145"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PKO06_07070"
     CDS             complement(<1..1808)
                     /locus_tag="PKO06_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV69437.1"
                     /translation="MMNKQPQTDLSSASGVSKTGTEPRERRRSSADTQIRELVNVGVA
                     LSHERDSATLLELIVSAARRLTGADAGTLYVVDKSTQTLHFKILQNETLGTRINAVKD
                     GGVAIPPPVPLFIEGEQNLANVSSSVALSGKTVNIPDVYQAEGYNFSGVKSYDAASGY
                     HSKSMLVVPMRDHENEIIGVLQLLNAVDPDSRAFVPFSENDAYLVTSLASQAAVAITQ
                     KRLIEQLSEAYVKTERSNVELKTELQTSTSVRRFGMIFSVCLLIAGGAAYAYFFDQNL
                     LVALRQPVEISEKSPAYGEQGRPVVTVPVERRRMTTTVALTGQVEPLKQMNVVSPFTG
                     KVLERSFEFGQTVTEGDILLRLDTTELQVQLREVTAAYMKEQERLNEITNWDKHRDVT
                     QARTNEIRAKASLATAKRKLEETNVLFEKGIVSKMELEAAAEAHENQILGLRTAQEDL
                     ASTLKRGDANAVRIAEMSLRNARTRLDEVSGRIAQAEIRAIQNQIIQHEQKKAARLKG
                     TLAANEQMLDEERRRAAEYSQLMAKEQARAAQYSQLADEERLKAAEAEKLAEQERLKA
                     LEAERVADSLRIEAKKRQEEARNKLNALQSKLIQVT"
     gene            complement(1805..3520)
                     /locus_tag="PKO06_07075"
     CDS             complement(1805..3520)
                     /locus_tag="PKO06_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69438.1"
                     /translation="MIERYSSSSPEAAAVHRVKKHVSRKVCGLSILLLLLGVATNAWG
                     SEETESQKLSTAHSSDTNVMTDVMAQASAPAPLPMSLDECVALAVRRNTTIRLAYMDR
                     VLYKYNFTTASDYAFRPNVSLSTTAGRNGARSEAQLETTSGADQVSSTLSVGQRLTTG
                     GSVSFSAKPWSRARSRSQRDDEESMTSKNIDRSWSVNLSQPLLKGAGHAYATADRRFA
                     RLGEIEDVLRLKETVMGQVTIVIKAYRALLQAKWTREINASALARARQQYQMNQVLIE
                     MGRMPRMELIQSESDVANRELSYQLAVNGYDQARISLLKLLDLNVETLIEPVEDLALP
                     EFRLDEDAVLKRIHAHQPSYLTTLLNVERVKISLMRARSEQHWDLNLTGSYERARSDV
                     IPGDATKNAVWQVGLALDIPLYGSLKRGIRSSLLTAENELRKMLIQLQKTEDDLRLEV
                     RDTLRQIRTNAQQITLAARARELSEKKLEIELEKLKAGKTTNFQLVTYQDELRNLKIN
                     ELSARIAYLNALTDLDRTMGMTVNTWGIALNADQPARGATPEPVTGETKAIEDSGTPA
                     PMTSP"
     gene            3756..>4190
                     /locus_tag="PKO06_07080"
     CDS             3756..>4190
                     /locus_tag="PKO06_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNV69439.1"
                     /translation="MKRITAAAVQFAVEPMAVEKNMKRTWEMIQECHRVSKANLIVLP
                     ESVTTGFTPIGTVQDLWKAVDTIPGRLTSEVERWAKLLDAYIVFPTYERGKKETTVYN
                     SAALIGPEGIIGVYRKTHPFPTERREAGGWTTPGSKACCLETP"
BASE COUNT          887 a         1217 c         1099 g          987 t
ORIGIN      
        1 tgagtcactt ggatcaattt gctctggagc gcattcaact tgttgcgcgc ctcttcttgc
       61 cgttttttcg cttcaattct caggctatct gcaactcgct cagcctctag cgctttaagg
      121 cgctcttgct cagccagttt ttcagcctct gccgccttga ggcgctcttc atcggcgagt
      181 tgagaatact gcgcagcgcg ggcctgttct ttcgccatca gttgggagta ttcagcagcc
      241 cgtcgacgct cttcatcaag catctgctcg tttgccgcga gtgttccttt aagacgagca
      301 gcttttttct gttcgtgttg aataatctga ttctgaatgg cgcgaatctc agcctgtgca
      361 atgcgcccag acacttcatc aaggcgtgtc ctggcgttac gtagcgacat ttcggcgatc
      421 cggacggcgt tagcgtcgcc acgtttcaga gtgctcgcca gatcctcctg agccgttctc
      481 agaccgagaa tctgattttc gtgcgcttcc gcggctgcct cgagttccat tttcgacacg
      541 atgcccttct cgaagagaac gttcgtttct tcgagttttc gtttggccgt cgccagactc
      601 gctttggctc gaatctcatt ggtacgcgcc tgcgttacgt cacgatgttt gtcccagttg
      661 gtaatctcgt tcagcctttc ctgctctttc atgtacgctg ccgtcacctc gcgtaactga
      721 acctgcaact cggtcgtatc caggcgcaga agaatgtcgc cctccgtgac ggtctggccg
      781 aactcaaagg atctttcgag cacctttccg gtaaacggac tcaccacgtt catctgcttc
      841 agcggctcga cttgtccggt gagcgcaacg gttgtcgtca ttcgacggcg ttcgacggga
      901 acggtgacga ccggccgacc ttgttcaccg tacgccggtg atttttccga gatttccacc
      961 ggctgacgca aagcaaccaa gagattctga tcgaaaaagt atgcataggc tgcacccccg
     1021 gcgatcagca ggcatacaga aaagatcatc ccgaatcgac gcacgctggt cgacgtctgc
     1081 agttccgtct tcaactcgac gttcgagcgc tcggtcttga cgtaggcttc actcagctgt
     1141 tcgatcaggc gtttctgggt aatcgcaacg gccgcctggg aggccagtga cgtgaccaga
     1201 taggcgtcgt tctccgagaa gggaacgaat gcacgtgaat ccggatctac ggcattgagc
     1261 aactgcaaca caccgatgat ctcgttttcg tgatcacgca tcggaacgac cagcatggat
     1321 ttcgaatggt atccggaagc agcatcgtag gatttcacgc cagaaaagtt gtatccctcg
     1381 gcctgataca catcagggat gttgacggtt ttcccgctca gagcaacact cgaagacacg
     1441 tttgccaggt tctgctctcc ctcaataaac aaaggaaccg gcggaggaat ggcaacacca
     1501 ccgtctttca ctgcgttgat cctggtaccg agagtttcgt tctgcaggat cttgaagtgc
     1561 aacgtctgcg tggatttgtc gaccacatag agggttccgg catccgcacc ggtcaaacgg
     1621 cgcgcggcac tgacgatcag ctcgagcaac gtcgcggaat cccgctcatg cgacagagca
     1681 acccccacgt tgaccaactc gcgaatctgc gtatctgccg acgaacggcg acgttcgcga
     1741 ggctcagtac cagttttcga aacacctgag gccgatgaca aatctgtttg cggttgcttg
     1801 ttcatcatgg cgacgtcatc ggcgccggag tgccggaatc ctcgatcgcc ttcgtctcgc
     1861 cggtcaccgg ttccggggtg gctccacgcg ctggttgatc agcattgaga gcaatccccc
     1921 aggtgttgac cgtcatcccc atcgtacgat ccagatcggt caaggcattg agataagcga
     1981 tacgtgcaga aagctcattg atcttgagat tgcgcaactc atcctgatag gtcaccaact
     2041 gaaagttcgt cgtcttcccc gccttgagtt tttccaactc gatctccagt ttcttttccg
     2101 agagttcacg ggctctggca gccagcgtga tctgttgtgc atttgtacga atctgcctga
     2161 gtgtatcacg aacctcgagt ctcaaatcgt cttccgtctt ctgcaactga atcagcatct
     2221 tccgcaactc gttctccgcg gtcagcagtg aacttctgat ccctcgcttc agacttccat
     2281 acaggggaat gtcgagagcg aggccaacct gccagacggc gttcttcgtt gcatcaccgg
     2341 gaatcacatc actccgggct ctttcgtacg aaccggtgag attaaggtcc cagtgttgtt
     2401 cagaacgagc acgcatcagg gaaattttca cgcgctcgac attcagcaag gtggtcaggt
     2461 acgagggctg atgcgcgtga atccttttca ataccgcatc ttcgtccaga cgaaactccg
     2521 gcagagcgag atcctccaca ggttcaatca gagtttcgac attcaggtcg agcagcttca
     2581 gcagagagat tctggcttgg tcatacccat taaccgccaa ctgatagctc agctcgcgat
     2641 tcgcgacatc cgattcagac tgtatcagtt ccatcctggg catacgcccc atctcgatca
     2701 gcacctgatt catctggtac tgttgccggg cccgcgccaa ggcgctcgca ttgatttccc
     2761 gggtccattt tgcctgcaac agtgcacgat acgctttgat gacaatcgtc acttgcccca
     2821 tgacggtttc tttcagccgc aagacgtctt cgatttcacc caaacgggca aagcgacgat
     2881 cggcagtcgc ataggcatgg ccagccccct tgagaagagg ttgcgacaga ttcacgctcc
     2941 aggagcgatc aatgtttttg cttgtcatcg actcttcatc atcgcgctgc gaacgcgaac
     3001 gcgctcgaga ccacggcttt gccgagaatg acacactgcc ccccgtcgtc agtctctgac
     3061 cgacagataa cgtcgacgaa acctggtctg caccactcgt ggtttccagc tgtgcctctg
     3121 atcgggcacc gttccggccg gccgttgtcg aaagcgaaac gttcggacga aatgcatagt
     3181 cggaagccgt cgtgaaattg tatttgtaca agacacgatc catgtatgcc agacgaatcg
     3241 tcgtgttccg tctcacagcc aaggcgacac attcatcgag agacatgggc agaggggcag
     3301 gggcggacgc ctgtgccatc acgtcagtca tgacattcgt atctgatgaa tgggccgtcg
     3361 agagcttctg cgactcggtc tcctcggaac cccaggcgtt cgtggcaact cccaggagta
     3421 gaagcagaat gctcagtccg cacaccttcc tggaaacgtg tttcttgaca cggtgaacgg
     3481 cggcggcttc tggagaggag gaggagtacc tctcgatcat tctcaggact ccaagcggcg
     3541 gaaatagtgt acagacctgc gcatgatatg accgggtcgc gaagcgtgtc aaggaattgc
     3601 gagtctgcca gcgagtttca gccactccgt ggtatcaccc gcgtgaacca tgtgagaaaa
     3661 agtgtcgaga gccgctgttt atggtagaat agcgcggttc gatccgccct gtctgctgcc
     3721 aaacccgcag aagatcgtca tctcggagtt accatatgaa acgcatcacc gctgctgccg
     3781 ttcaattcgc tgtcgagccc atggctgtcg agaagaacat gaagcgcacc tgggaaatga
     3841 tccaggagtg ccatcgagtc tccaaagcca acttgatcgt gcttcccgag agcgtgacca
     3901 ccgggtttac cccgatcggc accgttcagg atctctggaa agctgtcgac acgatacccg
     3961 gtcgcctgac ctccgaggtc gaacgctggg ccaagctgct cgatgcctat atcgtgtttc
     4021 ccacttacga gcgcggaaag aaggagacca cggtctataa cagtgccgcg ctgatcggac
     4081 ctgaaggcat catcggcgtg tatcgcaaaa cgcatccgtt tccgaccgaa cgacgcgagg
     4141 ctggcggctg gacgacacca ggttcgaagg cctgttgtct cgagacacct
//
LOCUS       DAORHD010000274         4186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22874_length_4186_cov_4.471799, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000274 DAORHD010000000
VERSION     DAORHD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22874_length_4186_cov_4.471799"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PKO06_07085"
     CDS             <1..190
                     /locus_tag="PKO06_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69440.1"
                     /translation="ENDHCEENRFAQPLLQLIDGGKRAARKVSHIWKTADDHEWHRNP
                     LMYVGGFIFLLSLLILFW"
     gene            193..849
                     /gene="rpe"
                     /locus_tag="PKO06_07090"
     CDS             193..849
                     /gene="rpe"
                     /locus_tag="PKO06_07090"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011129948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNV69441.1"
                     /translation="MKPVLIAPSILSADFSELAGGLQQISAAGADWVHLDVMDGHFVP
                     NLTFGPPVIEALRKRSSLPFDVHLMIEQPERWLEQYRSAGADRISFHLEACPHAHRYL
                     CRVRELGAGSGIVLNPQTSICGLEYLLPVCDMVLLMTVNPGFGGQSCIEAVIEKIRAL
                     RHLIDRLHLPTLIQVDGGIDRTNAQTIINAGADVLVMGSAFFRDSDKSDLVRQLKHLT
                     "
     gene            1029..2408
                     /locus_tag="PKO06_07095"
     CDS             1029..2408
                     /locus_tag="PKO06_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69442.1"
                     /translation="MLQAPKDLPQLIRSFEAHALAHPEDLSLMFKLGRLYLRNRNHPE
                     ACRAYRQILQHDADNLQARVELALCEAQQRHFDEAQCLLEDALAREPASLAVLLACSR
                     VHEWQGHVEQQISFLLRAANAAPRRHDIHLQLAELLRRYGDFSGAIAEYRRALELQAD
                     LESARFALGVLLMKRGELNEATDHFQQIVSRNPSAHDAYFNLGQCHAQRRQWQQAAIA
                     LQAAARGLKDHFGLQMLLVQAWTNLGDLDRAMVILEQLAEKLPENEVVLLALADLYER
                     CGEVDSAAETYSLLTRRYPEKPDYTVKWVQSLLRLNRHAEVPYILQGLFQRHPGHIEG
                     HRLLGEAFIGQKQYKSARDEFAKTLMVNESYLPGLVGLIQVARLTDDAAEEYRLMQRL
                     IEHNPDDLDTLLRLGRLERDLGLPANLERFRRITNLAPDSVQAREAEYYLRHAQKERP
                     RPAARATTR"
     gene            complement(2424..3869)
                     /locus_tag="PKO06_07100"
     CDS             complement(2424..3869)
                     /locus_tag="PKO06_07100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69443.1"
                     /translation="MYREKRERYHLERQEHWERVHAHVAEPSLFARAYQRRIAEVYEH
                     RIAAGSSILEVGCGCGRLLRALRPSRGVGIDFSARAVETARRETDGESIVYHRLEAST
                     LRDFPAEPFDFIILSDVLNDLWDVQELLAALQRFSSPHTRLLINMQSHLWKWPLKVAQ
                     AVGMAQPTLEQNWLTVQDLRNLLHLTGWEFMESWPEVLFPADVPGMAPVLNRFIGKLF
                     PFYHFCLTNFVIARPPGLLRSNTGLPSVSVIVPARNEEGNIVDIFRRVPQMGRETELI
                     FVEGGSTDRTREKIEEEKQHFQRLPCSLYAQAGTGKRDAVRLGFDKARGDILMILDAD
                     LTVAPEELMRFYEALVSGKGEFINGVRLVYPLEKEAMQFCNMLGNRFFSQGFSWVLGQ
                     PIKDTLCGTKVLWRRDYERLVANRPYFGDFDPFGDFDLLFGAAKLNLKILDLPIRYFE
                     RQYGTTNISRWRHGFWLLLMLISGARRLKFV"
BASE COUNT          915 a         1275 c         1087 g          909 t
ORIGIN      
        1 tgagaatgat cactgtgaag agaaccgttt cgctcagccg ttgctgcagt tgatcgacgg
       61 aggcaagcgc gccgcacgga aggtcagcca catctggaaa accgcggacg accatgaatg
      121 gcaccgaaat ccgctcatgt atgtcggagg atttatcttc cttctgtctc tgctgatcct
      181 cttctggtaa cgatgaaacc ggtacttatc gctccatcca ttctctctgc cgacttttcc
      241 gagcttgccg gggggctgca gcagatctcc gcggcaggcg cagactgggt ccacctggat
      301 gtcatggatg ggcacttcgt tcccaacctg acctttggtc ctccggttat tgaggcatta
      361 cgcaaacggt catccctccc gttcgatgta catctgatga tcgagcaacc cgagcggtgg
      421 ctcgaacagt accgatcggc aggagcagac cgcatctcct ttcacctcga agcctgcccg
      481 cacgcgcatc gctatctctg ccgtgttcgc gaactgggcg ctggatccgg cattgtgctc
      541 aatcctcaga cttccatctg cggcctggag tatctccttc cggtctgcga catggtgctg
      601 ctcatgaccg tgaacccggg attcggcggc cagagctgca tcgaagccgt catcgagaag
      661 attcgcgccc tgcgccacct gatcgatcgt ctgcatcttc ccacgctcat tcaggttgat
      721 ggaggaatcg atcggacaaa cgctcaaacc atcatcaacg caggagcaga tgtcctcgtc
      781 atgggctcag ccttctttcg cgactcggac aaatccgatt tggtcaggca actcaagcac
      841 ctcacctgaa ccgcgagcca tcaccttcac ctcgaaacct cgcttgtcca acgacgaaca
      901 gacataatat ttcgtcgcgt tctgccgatg tatgttcaaa gggtcacttg gaatgcgtga
      961 cccgtgaccg tgtattcaga ttcgccggca accaggaaac ggacagaccg gaaagcgagg
     1021 cacacgacat gctgcaggcg ccaaaagacc tgcctcaact cattcgcagt ttcgaggcac
     1081 atgcgttggc gcacccggaa gatctgtcgt tgatgttcaa actcggccgt ctctatctgc
     1141 gaaatcgtaa tcacccggaa gcctgtcgcg cctatcgcca gatcctgcaa cacgatgcgg
     1201 ataatctgca ggcacgtgtc gagctcgccc tgtgtgaagc acagcagcgc cacttcgatg
     1261 aagcacagtg cctgctcgaa gatgctctcg cccgtgaacc tgcttccctg gcagtgttgc
     1321 tggcttgttc cagagtccac gagtggcaag gccatgtcga gcagcagatc agttttctgc
     1381 ttcgcgccgc caatgccgcg ccacgccgtc acgacattca cctgcagctg gccgagctgc
     1441 tgcgccgtta cggcgatttt tcgggtgcca tcgccgagta tcgtcgggcc ctggaactgc
     1501 aggctgatct cgagtcagcc cgattcgcgc ttggagtatt actgatgaag cgcggggagc
     1561 tcaatgaagc gaccgaccac tttcaacaga tcgtctcgcg taatcccagt gcgcatgacg
     1621 cgtatttcaa tctcggacag tgccacgcac aacgtcgtca gtggcagcag gctgcgatcg
     1681 cgctgcaagc tgcggctcga ggtttgaaag accattttgg cctgcagatg cttctcgttc
     1741 aggcatggac gaatctcggt gaccttgatc gcgcgatggt catcctcgaa cagcttgccg
     1801 agaagctccc cgaaaacgaa gtggtgctgc tggcgctcgc cgacttgtat gaacgctgtg
     1861 gagaagttga cagcgcggcc gaaacgtatt ctctgctgac tcgtcgctac ccggagaagc
     1921 cggactacac cgtcaaatgg gtacagtcgc tgctgcgcct caaccgtcat gccgaagtgc
     1981 cgtacatcct gcaggggctt ttccagcgac atcccggaca cattgaaggt caccggcttc
     2041 tcggggaagc attcatcgga cagaaacagt acaagtctgc tcgcgacgag tttgccaaga
     2101 ctctgatggt gaacgaatca tatctcccgg gcctcgtcgg attgattcag gtcgctcgac
     2161 ttacggatga tgccgccgaa gaatatcgtc tgatgcagcg tctgatcgag cataacccgg
     2221 acgatctcga tacgctgctt cgactggggc gattggagcg cgacctcgga ttgccggcca
     2281 atctcgagcg atttcgtcgc atcacgaatc tggcacccga ctccgtacag gcccgcgaag
     2341 ccgagtatta tctgcgacac gcgcagaaag agcggccacg tcctgcggct cgcgcgacga
     2401 cccgctgacg aaaaccggtt ggctcagacg aatttcagac gtcgagcccc tgagatcagc
     2461 atcagcaaga gccagaatcc atgccgccaa cgactgatgt tggtcgtgcc atattgtcgc
     2521 tcgaagtatc tgatcggcag atcgaggatt ttcaggttga gcttcgcggc tccaaacagc
     2581 agatcgaaat cgccaaatgg atcaaaatcg ccgaaatacg ggcgattggc gaccagacgc
     2641 tcatagtcgc gacgccaaag caccttcgtc ccgcacagcg tatccttgat cggctgcccc
     2701 agtacccacg agaaaccctg gctgaaaaac cggtttccca acatgttgca gaactgcatg
     2761 gcttccttct ccaggggata gaccagacgc accccattga tgaactcccc tttgccggag
     2821 accagagctt cataaaatcg catcagctct tccggtgcca cggtcagatc agcatccaga
     2881 atcatcagaa tgtcacccct ggccttgtca aacccgagtc tgaccgcgtc tctctttcct
     2941 gtacctgcct gtgcatacag agaacaaggc aatcgttgaa agtgttgttt ttcttcctcg
     3001 attttttccc tggttcgatc cgtcgatcca ccttcaacga agatcaactc ggtctcgcgc
     3061 cccatctgag gcacgcgccg gaagatatcg acgatgtttc cctcttcgtt tcgagccgga
     3121 acgatcaccg aaacagacgg aagacccgtg ttcgagcgca acaaaccagg aggacgcgct
     3181 atcacgaaat tggtcaggca gaaatgatag aacggaaaca gtttgccgat gaaacgattc
     3241 agcacaggcg ccatacctgg cacatcagcc ggaaagagca cttccggcca ggattccatg
     3301 aattcccatc ccgtaagatg caacagattg cgcagatcct gaaccgttaa ccagttttgc
     3361 tccaacgtcg gctgcgccat tcccacagcc tgagcgactt tcagtggcca cttccacagg
     3421 tggctctgca tgttgatcag cagacgggta tgcggcgacg aaaagcgttg cagagccgca
     3481 agaagttcct gcacatccca gagatcattc agcacatctg acagaatgat gaaatcgaac
     3541 ggctccgccg gaaaatctcg cagcgtcgat gcttcgaggc ggtgatacac aatcgattcg
     3601 ccgtcggtct cacgccgggc cgtctcaacc gcccgcgcgg agaagtcgat tcccacgccg
     3661 cgcgaagggc gcaacgcacg caaaagacgc ccgcatccgc aaccgacttc gagaatgctc
     3721 gatcctgcgg caatacgatg ctcatacact tccgcaattc ttcgctggta cgcccgcgca
     3781 aacagactgg gctcggcaac atgggcgtga accctctccc agtgttcctg acgttcgaga
     3841 tggtagcgct ctcgcttttc ccggtacatg aatcaagacc tctttcacgg tttcatacag
     3901 tcaccatcgc cattgtatcc gaaaactccc tgcgtcgtcg tcgttcgttt cgtgtttccg
     3961 gtccgcgcca tattttcttg tctcacgctt ctgacaagtg gtaaacagag agcatgcaga
     4021 cagctcgtac gcaattcgtg gcagcgtttt tcctctcgtt gattctcgca gcgatcgccg
     4081 tcctggttcc gtcacctgac gagcgtctga ggctctttcg cacgtatgga ttcctttcca
     4141 tctttctcac gtgcgtgctg tggctgcttt ccgtgcccga ccgacg
//
LOCUS       DAORHD010000275         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22884_length_4185_cov_3.789346, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000275 DAORHD010000000
VERSION     DAORHD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22884_length_4185_cov_3.789346"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKO06_07105"
     CDS             <1..555
                     /locus_tag="PKO06_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNV69444.1"
                     /translation="TPLFDDRDIRLLGRHNRENVMAAATAAILAGVPPAGLKQVVQTF
                     PGVEHRLEWVRERSGVAYVNDSKGTNCAASIIALQACTTPVILIAGGRDKGTDLRDWV
                     EAVRLKARGVVLLGEAKERFRAALEGVVPLKMAGTFADAVRLASDWALSGETVLLSPA
                     CSSYDMFPNFEVRGACFKDLVMKL"
     gene            580..1764
                     /gene="ftsW"
                     /locus_tag="PKO06_07110"
     CDS             580..1764
                     /gene="ftsW"
                     /locus_tag="PKO06_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNV69445.1"
                     /translation="MDEHRGFDHLLFFVVVALMGLGLIMVYSASSITSLTERADALYY
                     FKRQLLWVALGLLGMFWFARVPYRSLQSYALPLLLVALFFLILVLIPGVSREIGGARR
                     WLRIGGQGFQPSEFAKLVFVIYMAWSISAKQDRIKELVRGVLPDLAVMTIFFVLILKG
                     PNLSTAAIVAGTYFLMLFLGNGSLAHLSWMVGAGVVTLVGLIFEKGYRMKRFLAFLDP
                     WSSAKTSGYHIIQSLIAIGAGGVWGLGLGQSRQKFFLLPERHTDFIFAIICEELGLVG
                     GVTVVVLFGILIWRGFYVAVRAPDMFGFLLASGITVVIALQVLINLGVVLSLLPTTGV
                     TLPFVSYGGSSLLFLAVMVGILLNVSRFGSSQHAVVDHQRQPFIRWLNRSWERLSVRA
                     RR"
     gene            1716..2852
                     /gene="murG"
                     /locus_tag="PKO06_07115"
     CDS             1716..2852
                     /gene="murG"
                     /locus_tag="PKO06_07115"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNV69446.1"
                     /translation="MAQPFLGTSFCQGAAMKRILFSGGGTGGHIYPALAIREVILEKL
                     GTEVETAYMGVGDGMEAGIVARVPGLPFFPVRAQGMPRRLSLQWLTFPVRNALGFSDA
                     VRHLREFKPDLVVTTGGFVAFPCLMSAWLSGVPFVLHEQNAAMGFTNRLFASRAKRVL
                     LTYGEAYTETSERVTVTGNPVRGAFRHRTPVTGRFEKKPGEFWLLAVGGSRGAASLNK
                     ACVGFVRDVLPRYPEVKLIHIAGERDYENIRSQIPTMPNHILLPYLHEMREAFAVADL
                     LISRAGATILAEIACEGRPAILVPYPFATDNHQEKNARILEERGAAKVLLDHELSGER
                     LERLLVDLRHEGRLESMAAAMRLTRPENVEERIWKAIEECLTAP"
     gene            3036..3830
                     /locus_tag="PKO06_07120"
     CDS             3036..3830
                     /locus_tag="PKO06_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HNV69447.1"
                     /translation="MQKLVRYSAPVKFLLFLAVVFLLSYLAVVQLRHLFFATSYFELR
                     KIEIHGVKTLKEEDVLRLANVAPGHNVLSLNKDEVQSRILVHPMVRKAQIELSGLYTL
                     KIAIEERVPYVYVKSGAQFFEISDDGTVLAVEDVGSRDLPIITGIDVGDRKIGDSLLE
                     MDSFFEAMRWIRALDHPMLVRISELNFENQQNPYLFFVSGEKIIPKSLEDFKERFLFL
                     CALLDNLRKNNVEPDYLDMRAPSEIVVRPRRLKSAPEGRSEPLGHR"
     gene            3817..>4185
                     /locus_tag="PKO06_07125"
     CDS             3817..>4185
                     /locus_tag="PKO06_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNV69448.1"
                     /translation="MVTDDLLVGLDIGTTKVCAVIGSVRENGDLNVIGVGSAPTNDGV
                     RKGAIVDIDRTVDAITTAVDEAERTAGSGVEYVYVGISGAHITSVNSQGIVAIKGNSR
                     EITDEDVERAVENAKTAVSIP"
BASE COUNT          801 a         1064 c         1290 g         1030 t
ORIGIN      
        1 acccctctgt ttgacgatcg cgacattcgc ttgctgggtc gtcacaatcg tgaaaatgtg
       61 atggcggcgg cgacggccgc cattctggct ggagtgccgc ccgccgggct gaagcaggtg
      121 gtgcagacgt ttccgggggt ggaacatcgc ctcgaatggg tgcgcgaacg cagtggtgtc
      181 gcgtatgtca atgattcaaa gggaaccaac tgcgccgcat cgatcattgc cctgcaggcg
      241 tgcacaactc cggtgatctt gattgccggt ggacgcgaca agggcaccga tttgcgggac
      301 tgggtcgagg cggtgcgtct gaaggcgcgt ggagtggtgt tgctgggcga ggccaaagag
      361 cgctttcggg ctgctctcga aggggtggtg ccgctgaaga tggctggcac gtttgctgat
      421 gcggttcgtc tcgcgtccga ctgggcctta tccggtgaaa ccgtgcttct gtcgccagcc
      481 tgcagcagtt acgatatgtt tccgaacttc gaagtgcgcg gggcctgttt caaagatctg
      541 gtcatgaagc tgtagagtct ttcaggatga acgtttgaca tggatgaaca tcgcgggttc
      601 gatcacctgc tgtttttcgt ggttgtggca ctcatgggcc tcggtctgat catggtttac
      661 tcggcatctt cgattacatc actgaccgag cgtgccgacg ccctgtacta cttcaagcga
      721 caactgctct gggtcgctct gggtttgctt ggcatgttct ggtttgcgcg ggtgccctat
      781 cgatctctgc aatcgtatgc cctgccgctt ctcctcgtgg cgttgttttt tctcattctg
      841 gtactgatcc cgggggtgtc gcgcgagatc ggcggtgccc ggcgctggtt gcgcattggt
      901 ggtcagggat ttcagccttc ggagtttgcc aagctcgtct ttgtgattta catggcctgg
      961 tctatcagcg ccaaacagga tcgaatcaaa gaactggtgc gcggagtgtt gcccgatctg
     1021 gcggtcatga ccatcttctt cgtgctgatc ctgaaaggcc cgaacctgtc aaccgcggcc
     1081 atcgtggccg gaacctattt tctgatgctg tttctgggga acggttccct ggcccacctg
     1141 tcgtggatgg taggtgctgg agtagtcaca ctggtcggtc tgatcttcga gaaaggctat
     1201 cgcatgaaac gttttctggc ctttctcgac ccgtggagtt cggcgaaaac gtcgggatac
     1261 cacatcatcc agtcgttgat tgccattggt gccggcgggg tctgggggct cggtcttggg
     1321 cagagtcgtc agaagttctt tctgttgccg gaacggcaca cagacttcat ctttgcgatc
     1381 atctgcgaag agctcggctt ggtaggcggc gtcaccgtgg tcgtgttgtt cggtatcctc
     1441 atctggcgcg gattctacgt cgcggtgcga gcacctgaca tgtttggctt tcttctggcc
     1501 tcgggaatca ccgtggtcat tgccctgcag gttctgatca acctgggtgt cgtactcagt
     1561 ctgctgccga cgaccggagt gacgcttccc tttgtgagtt acggaggatc aagtctgttg
     1621 tttcttgccg tcatggtagg cattctgctg aatgtctcgc gttttggttc gagtcagcat
     1681 gcagtggtcg atcatcagag gcaaccgttc attcgatggc tcaaccgttc ctgggaacgt
     1741 ctttctgtca gggcgcggcg atgaagcgca ttctcttcag cggcggtggc accggtggcc
     1801 acatctaccc ggctctggcg attcgggaag tcattctcga gaagctcggt accgaggtcg
     1861 aaacggcgta tatgggcgtt ggagacggaa tggaagccgg tatcgtggct cgtgttcccg
     1921 ggttgccgtt cttcccggtg cgggcgcagg ggatgccgcg gcgcctgtct ctccagtggc
     1981 tgacgtttcc ggttcgtaat gcactggggt tcagcgatgc ggttcgccat ctgcgtgagt
     2041 tcaaacccga cctggtggtc acgaccggtg gtttcgtggc ttttccttgt ttgatgagcg
     2101 cctggctgag tggtgtgcca tttgttctgc acgaacagaa tgcggcgatg ggcttcacca
     2161 accggttgtt tgcctctcgg gccaagcgcg tgctgctgac ctacggtgaa gcctatacgg
     2221 aaacctcgga gcgggtgacg gtcacgggaa acccggttcg tggggccttt cggcatcgca
     2281 ctccagtcac gggaagattc gaaaagaaac caggcgaatt ctggctgctg gcggtgggcg
     2341 ggtcgcgtgg cgcggccagc ttgaacaaag cctgtgtcgg atttgtccga gacgttcttc
     2401 cgcgatatcc tgaggtgaag ctgattcaca ttgccggaga acgcgactat gaaaacatcc
     2461 gctcacagat tccgaccatg ccgaatcata tcctgttgcc ctatctgcac gagatgcgcg
     2521 aggccttcgc cgtcgccgat ctgctgatct cacgcgccgg agcgaccatt ctggccgaga
     2581 ttgcctgcga aggccgcccg gcgattctcg tgccgtatcc gttcgcgacg gacaatcacc
     2641 aggaaaaaaa cgcgcgtatt ctcgaagagc gtggagcggc gaaggtgctg cttgatcacg
     2701 agttgtccgg ggaacgtctg gagcgtttgc tggttgacct gcgacacgaa gggcgtctcg
     2761 agagcatggc agcggccatg aggttgacgc ggccggaaaa tgtcgaagag cgaatctgga
     2821 aagcgatcga agaatgtctg accgcaccgt gatgttgcat gcggatcttc gtgtttgtgg
     2881 acctgtactg aaatcgaaca agcaataagg ggaagggaac cagtatcgtg gcggagcagc
     2941 cggagcgacc caagcgaaac attgttctgc ccaaccgtgc cggtttgtca gatcagacgg
     3001 tcgagcggtt gcgcaatcgt gatattcgca ttcgcatgca gaaactggtg cgctactccg
     3061 cgccggtcaa atttctgttg tttctcgcgg tggtctttct gctttcgtat ctcgccgtgg
     3121 ttcagttgcg tcacctcttc tttgccacct cgtatttcga gttgcgtaag atcgagatcc
     3181 atggggtgaa aacactgaaa gaagaagatg tgttgcggct tgcgaatgtt gcgccggggc
     3241 acaacgtctt gagtttgaat aaggacgagg tgcagagtcg gattctggtg caccccatgg
     3301 ttcgaaaggc tcagattgag ctttcaggct tatatacgct gaagattgct atcgaagagc
     3361 gggtgcccta tgtctatgtc aagtcaggtg cgcaattttt cgaaatttct gatgacggca
     3421 ccgtgcttgc ggttgaagat gttggaagtc gtgatctacc gatcattacc ggcatcgatg
     3481 tcggtgatcg taaaatagga gattcgcttc tggagatgga ttccttcttc gaggcgatgc
     3541 gatggatccg cgctctcgat catccgatgc tcgtgagaat ctctgagtta aatttcgaaa
     3601 atcagcagaa tccttatctg ttctttgttt ccggggagaa gatcatccca aagagccttg
     3661 aggacttcaa agagcgcttt ctttttttgt gtgcccttct tgacaacctc agaaagaata
     3721 atgtagaacc agattacctt gacatgaggg caccgagcga aatcgtcgtc aggccccgac
     3781 gtctcaaaag cgcacccgaa gggagaagcg agcctcttgg tcaccgatga cctgctggtc
     3841 ggattggata ttggcacgac gaaggtttgt gccgtcatcg ggagcgtgcg cgaaaatggc
     3901 gatctcaacg tgattggtgt aggcagtgct ccaacgaatg acggcgtgcg taaaggagcc
     3961 attgtcgata tcgaccgcac ggttgacgcc atcaccacag cggttgatga agccgagcga
     4021 acagccggca gtggcgtcga atacgtctac gtcggaattt cgggtgcaca tattacctcg
     4081 gtgaacagcc agggcatcgt cgctatcaag ggaaactccc gcgagatcac agacgaagac
     4141 gtcgaacgcg ccgtggagaa tgcgaaaacc gccgtatcga ttccg
//
LOCUS       DAORHD010000276         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22895_length_4183_cov_3.154312, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000276 DAORHD010000000
VERSION     DAORHD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22895_length_4183_cov_3.154312"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKO06_07130"
     CDS             complement(<1..211)
                     /locus_tag="PKO06_07130"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNV69449.1"
                     /translation="MRAVVQRVGPTFLTVDGREISRIDEGLVCYLGVQKGDGDTDLFW
                     MTKKVSGLRVFSDTQGKMNLSVLDLG"
     gene            complement(219..1136)
                     /gene="secF"
                     /locus_tag="PKO06_07135"
     CDS             complement(219..1136)
                     /gene="secF"
                     /locus_tag="PKO06_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV69450.1"
                     /translation="MKFRFMDYRNQLFTFSAVLTIISLLLFIGKGFNYGIDFKGGSIL
                     HLRFNEPMNEPKIRETFGKMPNLYFKPDQLIIQAVGGDASGREFIVQYPALVREDADS
                     SRIHSEILEQLRKLAPFSEDALSVANVGPTVGGEMKQQGFLAAVISIIGILLYLAWRF
                     EFQSATGAIISLVHDLVVVSGAVCLLGIEFDVTVLAALLTMLGYSVNDSIVVLDRIRE
                     NRRLVRTASFAELINDSINQTLGRTINTTITTLFTLVTLLLFGGQSIHNFALTLTIGV
                     AFGTYSSIFIAAPVLLLMTGDKVGKKVTA"
     gene            complement(1165..2877)
                     /gene="secD"
                     /locus_tag="PKO06_07140"
     CDS             complement(1165..2877)
                     /gene="secD"
                     /locus_tag="PKO06_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNV69451.1"
                     /translation="MRSDHKFKLIVIFVVFLLTLLYTFPSTPWWESSFGLLTRDEQVA
                     VPFAQLVYKDGDDGSSGIIEFAVVHGASIFTKNSSLRPEDVLTSVADTSRRRLMDLGY
                     EAKIVNPDYSKGTLAIRVAKKEQKIGKADLPKIMAQTNLYASLPLGLSYIFPQSKITL
                     GLDLKGGIDLVYQVDLSSVSKDDNPADAVKRSVEIIRNRIDIYGVAEPQIKEQEGHRI
                     RIQLPGVRDIERVKGLIQSTAMLKFHIVLNAGASVAELEPVNPETEMVLISAAQRSAQ
                     GELGAAQGLYYKLKREPEVTGRDLKYAQEGFDDMGGPMVHLAFNPEGAIKFSKATGQH
                     LGEKLAIVLDNKVHSAPEIQSKITGGLAQITGRFSLDEAKNLAIVLRAGALPANLNLI
                     ESRVVGPTLGAESIRAGWQAGLVGAFLVVVFMITYYKAAGVMADLAVLFNILITFSAL
                     VFFGGTLTLPGIAGLVLSVGMAVDANIIIFERIREELRSGKTVRASIESGFDRALTCV
                     IDSNVTTILTVIVLYVFGSGPIRGFATTLGIGLVANIFTAIICVKLAMDLFYGRAKSV
                     EMSI"
     gene            3153..>4183
                     /locus_tag="PKO06_07145"
     CDS             3153..>4183
                     /locus_tag="PKO06_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69452.1"
                     /translation="MPHLFPAAWRRVLAFLVVLTAVCSLASGSSYDSMVKKGRDHFGL
                     YQWAQARKSFEQAISLDPTNPDAYYHLGLTLRKLNDLQGAINAFEQALKYKTEEIDCP
                     RHLATIYIQFAKDAKARNDHAAMVDNLRKSIVAYPNNTSVAASLFDFLANDGEWKEVA
                     RLGELVRNANREALDAGDDKDLQRALVMTARAYISLQEHARARDFLRLAEMIRHPNDE
                     IAKLKSQIGQTSQAVANNFFSEGKALFEERKYKQALEVLRKAQGAEPGNSEISNLIEL
                     TEKRITVADFSQLAREAEKAGKKLTNLFIFVEEVFSEGQEVLILVTELTMNYFAAKFI
                     SRFGSKEYFA"
BASE COUNT         1009 a         1194 c         1107 g          873 t
ORIGIN      
        1 gccccagatc cagtaccgag aggttcatct tgccctgagt gtcggaaaaa acacgaaggc
       61 ctgagacttt cttggtcatc cagaagagat ccgtgtctcc gtcccccttc tggacgccca
      121 gatagcacac caggccttcg tcaatgcgag agatctcgcg accgtcgact gtcaggaagg
      181 tgggcccgac cctttggacg acggcgcgca tgagcgaatc aggcagtgac cttcttgccg
      241 actttgtcgc cggtcatgag caacagcacg ggagcggcaa taaaaatcga ggagtaggtt
      301 ccgaacgcca caccgatcgt cagcgtcaga gcgaagttgt gaatcgactg accgccgaac
      361 agcaacaggg tgacaagggt gaacagagtg gtaatcgtgg tattgatcgt acgacccagg
      421 gtctggttga tggaatcatt gatcagttca gcaaaactcg ccgtgcgtac cagtcgccga
      481 ttctcgcgaa tacgatcgag aacgacaatc gagtcgttaa ccgagtatcc cagcatggtc
      541 aacagagccg cgagcaccgt gacgtcaaat tcgataccca gcagacaaac cgctccagat
      601 accacgacca agtcatgcac cagcgagatg atcgctccgg tcgccgactg aaactcgaaa
      661 cgccaggcca gatacagcag aatgccaatg atactgatga cggccgccag gaacccctgc
      721 tgcttcatct caccaccaac ggtcggaccg acgttggcta ccgaaagagc gtcttcggaa
      781 aagggagcga gcttgcgcaa ctgctcgaga atctcgctgt gaattctgct cgaatcggca
      841 tcttcacgaa ccaatgccgg gtattgcaca atgaactcgc ggccactggc gtcgccgcca
      901 acagcctgaa taatcagctg atcgggcttg aaatacagat tcggcatttt gccgaatgtt
      961 tctcggatct tcggttcgtt catcggctca ttgaagcgca gatgcagaat ggatccgccc
     1021 ttgaaatcga taccgtagtt gaagcctttc ccgatgaaga gcaggagaga aatgatcgtc
     1081 aatacggcgg agaaggtgaa tagttgattg cgataatcca tgaagcggaa tttcatctcg
     1141 ttgttccttc ctgttccgtt tgtatcagat gctcatctcg acggacttgg cgcgaccgta
     1201 gaagagatcc atggccagct tcacgcagat aatggcggtg aagatgttcg cgaccaggcc
     1261 gatgccgagg gtagtagcga atccgcgaat gggacccgac ccgaagacat acagcacgat
     1321 aaccgtcaga attgtggtca cgttcgagtc gatgacacag gtcaacgcac gatcaaaccc
     1381 cgactcaatc gaagcgcgaa ccgtcttgcc agaacgcagt tcttcacgta tgcgctcgaa
     1441 gatgatgatg ttcgcatcaa cggccatacc cacggaaagc accagaccgg cgatgccggg
     1501 cagtgtcagc gtgccaccga agaacaccag ggcagagaag gtaatcagaa tgttgaagag
     1561 aaccgccaga tcggccatca cacccgcggc tttgtaatac gtgatcatga atacgaccac
     1621 caggaacgct ccgaccagcc cggcctgcca accggcgcga atcgattcgg ctcccagagt
     1681 cgggccgaca acgcggcttt caatgaggtt caggttcgcg ggcaaggcgc cggcgcgcag
     1741 cacgatcgcc agattcttgg cctcgtcgag tgagaagcga ccggtgatct gggcaagacc
     1801 acccgtaatc ttgctctgga tttccggggc gctgtgaacc ttgttatcaa gcacgatcgc
     1861 cagcttttca cccagatgct gtccggtagc tttcgaaaac ttgatcgcgc cttccgggtt
     1921 gaaggccaga tgcaccattg gcccgcccat atcatcgaac ccttcctgag catacttcag
     1981 atcgcgacca gtgacttcag gctctcgctt gagtttgtag tagagaccct gggcggcacc
     2041 cagttcgccc tgcgcactgc gttgagcagc cgagatcagc accatttcgg tttcggggtt
     2101 gaccggttcg agttctgcta ccgaagcccc ggcattcagc acaatgtgga acttgagcat
     2161 cgccgtcgac tgaatcagac ctttcacgcg ctcgatgtca cgcacgccgg ggagctgaat
     2221 gcgaatgcgg tgaccttcct gttccttgat ctgcggttct gccacaccat agatgtcgat
     2281 gcggttgcga atgatctcga ccgaccgttt caccgcgtca gccggattgt catccttgga
     2341 gacggaactg agatccacct gatagaccag atcgataccg cccttgagat caaggccgag
     2401 cgtgatcttc gattgcggga agatgtagct cagacccagc ggcaaggaag cgtacaagtt
     2461 cgtttgagcc ataatcttgg gaagatcggc ttttccgatc ttctgttctt tcttcgcgac
     2521 ccgtatcgcc agcgtgcctt tgctgtaatc gggattcacg attttcgctt cgtatccgag
     2581 atccatcagg cgccggcgtg aggtgtcggc cacgctggtg agcacgtctt caggacgcag
     2641 actggagttt ttcgtgaaaa tggaagcgcc atgaaccacg gcaaattcga tgatcccgga
     2701 acttccgtca tcgccatctt tgtaaaccag ctgggcaaac ggaactgcca cctgctcatc
     2761 acgggtcagc agcccgaacg agctttccca ccagggcgtt gacggaaacg tatacagcag
     2821 ggtgagaaga aagacgacga aaatgacgat cagcttgaac ttgtggtcac tgcgcatgaa
     2881 acgaagcccc ctagaatatg gtgcaactgt aaagaggggt gaattatacc caagcccccc
     2941 acccaagtca acacattgcg ccgtccctca cgggacgaag cctgttcccg catcatgctt
     3001 cggcatgtga gccccactcc tgcgctctct ctcaatttat ctggtaccag cgtcgcccgt
     3061 acccgcatct gccagagtct atctcgctct cgaccaatgt cggacaatga ttccggttga
     3121 ccctcggttt ctggtcatgg tatggttgga tcatgccgca cctctttcct gccgcctggc
     3181 ggcgtgtttt ggctttcctc gtcgttctca cggctgtctg cagcctggcg tccgggtcct
     3241 cgtatgacag catggtcaag aaaggacgcg accattttgg tttgtaccag tgggctcagg
     3301 cgcgcaaaag ctttgaacag gcaatctctc tcgatccgac gaaccccgat gcctactatc
     3361 atctcggttt gactctgcgc aagctgaacg acctgcaggg tgccatcaat gccttcgagc
     3421 aggctctcaa atacaaaacc gaagaaatcg actgtcccag acatctggcc accatctata
     3481 tccagtttgc caaagacgcc aaagcccgca atgatcacgc cgccatggtc gacaatctcc
     3541 gcaagtccat cgtcgcgtat ccgaacaaca ccagtgtcgc ggccagtctg tttgattttc
     3601 tcgccaatga cggcgagtgg aaagaggtcg cccgccttgg cgaactggta cgcaacgcca
     3661 accgcgaagc acttgacgcc ggcgacgaca aagatctgca aagagctctg gtcatgacgg
     3721 ctcgcgccta catttccctt caggagcacg cgcgagcccg cgactttctg cgtcttgccg
     3781 aaatgatccg gcatccaaac gacgaaatcg ccaagctgaa gtcccagatc ggacagacct
     3841 cacaggcagt cgccaacaac ttcttctctg agggcaaggc cttgttcgag gagagaaaat
     3901 acaagcaagc cctggaagtc ctgagaaaag cacagggcgc tgaacccgga aacagtgaga
     3961 tcagcaacct tattgaactc accgagaaac gcattaccgt tgcggatttc agccaactgg
     4021 cccgcgaagc cgaaaaagcc ggaaagaaac ttaccaacct gttcatattt gtagaggaag
     4081 tgtttagtga gggtcaggaa gtgttgatct tggtaacgga gttgaccatg aactattttg
     4141 ccgccaaatt catcagccgt ttcgggtcta aagaatattt tgc
//
LOCUS       DAORHD010000277         4175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22967_length_4175_cov_3.447573, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000277 DAORHD010000000
VERSION     DAORHD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22967_length_4175_cov_3.447573"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="PKO06_07150"
     CDS             <1..1015
                     /locus_tag="PKO06_07150"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69453.1"
                     /translation="LCAIFPQEEVDRRVHDLRWRLRMGAGLSLIAALLVGILLAQALV
                     RPMEDLSVGIASLARKDFETQVPVRQRDEFGLLAHAFNQMTIELKDLNMAGAVQSSLI
                     PHGYPQVSGYSLDGKVVFAGDLGGDCLDCRPLPDGRIFFLIGDVSGHGAASALIMAFT
                     RATVTLWISEGRGDLSRLANRLQTMLSSLPGRRLFLALFCGILDSQTHRIDGLCAGHP
                     FPITVSDKGLTRFHGLPAYPLGSHRGSRGGVPVTLQLEPHEVLLLYTDGLIEARDVAG
                     DELGYSGLESLLQATVAHSVEAAHPAAEVLQVLVQQHRRRSPSAEDDVSLLAIVRRGG
                     GTA"
     gene            1012..3906
                     /locus_tag="PKO06_07155"
     CDS             1012..3906
                     /locus_tag="PKO06_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV69454.1"
                     /translation="MSQPSYLRLGQMSGRYSYLILVIGFVSFCFALILQTLTLWETRW
                     REQERSARRQRFDEAVHHREFAPSFALFPWKVMLRVRNNWVNHLEIQEFCATHSRLLG
                     MKASYLQFQESHDLLVYPPATDTKELFRDVFTSIALPEPQFSHADRRLQRPLREEFGI
                     EGGLAFLRWHLDEFFPFTRHGRPGIGIMSLWRDGTGIVILMDDVPERMKREVERYAKR
                     RKALSTFVGKAMPEREVWLPPPGVGSDALRLVYQLTTRAGVSEKTYEGREWLILRNRQ
                     GFVDFWVEPAFDLPWLRSLRVVLWVLYPLLLWWTVRLVRDLFRAGEEATRQQVLSLRT
                     QVRFFLGAVTLLPLGSAIALALMLETDMGNNLRERAFSTGTARLQSLYLGLDGHLVRT
                     ARRSREFADLIASYPFDQELIRREAKRHEDLDTFAWYAIMDGSNRLLMGDMHIWRPDI
                     YNLMEKLCRLVVRRFVPHRVVETAGRVETIEVFLEEVATNEQLGWAEVLENPNVVQKH
                     QLGGASGNLYWTVFPHLSTGPAFLACALDTDRLVRSFFEPILASSPSAADFVFGIHQR
                     HFEIVAPVAKREIRPMLPLVLKTHKTATTVQGEVKTGTRRWWVVAMPENYSGYYILAW
                     AADADQALLALRPLRWALFAGLLATLAVVFGVYRLLVNMVLAPIGDLRTGIAAIRQRQ
                     TGVRLPIRRADEFGELAMLFNRTLSGLQELELARLVQTQLLPARIPEVAGYTLAAENL
                     TATELSGDYYDLVPLGDDTLLAVIGDVTGHGAPAALAMAMARAIIAARAADGEKSPAE
                     YLRSLNEVFFQDMKPTRRFMTFLAGRLHMRAHKIEFANAGHNYPLVYRAAAKSVETPE
                     MPGLPLGIRRQVPRTSHEIDLLPGDALVYYTDGYTESLMPDGTQVGPERLEAMLQDEA
                     PRAADAARLLKALVERLDAIRRPGPRDDDVTMLVISRNRLPAPTCASEPG"
     gene            complement(3881..4084)
                     /locus_tag="PKO06_07160"
     CDS             complement(3881..4084)
                     /locus_tag="PKO06_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNV69455.1"
                     /translation="MTTCANKSKNEKTCTCTYEPCSRKGICCECIAYHRRNAEIPGCL
                     FPKANEQTYDRSIDAFIRAHSRT"
BASE COUNT          781 a         1131 c         1326 g          937 t
ORIGIN      
        1 tctctgcgcc atattcccgc aggaggaagt ggatcgtcgg gtgcatgacc tgcgctggcg
       61 actgcgcatg ggagccggtt tgagccttat tgccgctctt ctggtcggca ttctgctggc
      121 tcaggccctg gtgcgaccga tggaggatct ctccgtcggc attgcgtcgc tggcgcggaa
      181 agactttgaa acgcaggttc ctgtacgaca acgcgatgaa ttcgggttgc tggctcatgc
      241 gttcaaccag atgacgattg aactcaagga cctcaacatg gctggtgcgg ttcaatcgtc
      301 gctgataccg cacggatacc cgcaggtgag cgggtacagc ctggacggaa aggtggtgtt
      361 tgcgggtgac ctgggaggcg actgtctcga ctgtcgacct cttcccgacg gcagaatctt
      421 ctttctcatc ggtgacgtga gcggccatgg tgcggcttcg gccctgatca tggcgttcac
      481 ccgggcgacg gtgacgttgt ggatcagcga gggacgcggt gatctgtcca ggctggcgaa
      541 tcgtctgcag accatgctga gctcgttgcc tggtcgacga ttgttcctgg ctctgttctg
      601 cggcattctc gattcgcaga cgcatcgtat cgatggattg tgtgccggtc atccctttcc
      661 gattaccgtc agcgacaaag gtctgactcg ttttcatggc ctgccggcct atccgttagg
      721 aagtcatcgt ggcagtcgcg ggggagtacc ggtaacgtta cagctcgagc cgcatgaagt
      781 gctgttgttg tataccgatg gattgattga agcccgcgat gttgccggag acgagctggg
      841 ctactcggga ctggaatccc tgctgcaggc caccgttgcc cacagtgtcg aagcggcgca
      901 tcctgccgca gaagttctgc aggtgctcgt tcagcaacat cgtcgtcgat cgccatcggc
      961 ggaagatgac gtatcgctgc tggccatcgt tcgccgcgga ggcggaaccg catgagccag
     1021 ccatcatatc tgcgtttggg ccagatgagc ggcaggtatt cctacctgat tctggtcatc
     1081 ggtttcgtgt cattctgttt tgccctgatc ctgcagacgc tgacgctctg ggaaacccgt
     1141 tggcgtgaac aggagcgctc cgccagaagg cagcggtttg acgaagcggt gcatcatcga
     1201 gagtttgcac ccagctttgc gttgttcccc tggaaagtga tgctgcgcgt gcgtaacaac
     1261 tgggtgaacc acctcgagat tcaggagttc tgtgcgacgc actctcgcct gttgggaatg
     1321 aaagcgtcct atcttcagtt tcaggagagc cacgatctgc tggtgtatcc gccggcaacc
     1381 gatacgaaag agttgtttcg cgacgtcttc acgtcgatcg cgctgcctga acctcagttt
     1441 tcacatgctg atcgacgtct gcagcgaccg cttcgcgagg agttcggaat cgagggcggg
     1501 ctggcgtttc tccgttggca tctggatgag ttctttccat tcacccgcca tggccgtcca
     1561 gggatcggca tcatgagtct ctggcgagat ggaaccggca ttgtcattct catggatgat
     1621 gtgccagagc ggatgaagag agaggtcgaa cggtatgcga aacggcggaa ggcgctgtcg
     1681 accttcgtgg gcaaagccat gccggagcgt gaggtctggt tgcctccacc gggagtcggc
     1741 agtgacgctc ttcgcctggt gtaccagttg acaaccagag cgggagtctc ggagaaaacg
     1801 tatgagggac gtgaatggct gattctgcgg aatcgtcagg gcttcgtcga cttctgggtg
     1861 gaaccggcgt tcgatctgcc ctggctgcgc agcctgcgtg ttgtgctctg ggttctgtat
     1921 ccgctgctgc tatggtggac agtgcggttg gttcgcgatc tgtttcgtgc cggcgaagaa
     1981 gcgaccaggc agcaggtact ttccttgcgt acccaggtcc gattctttct gggcgcggtc
     2041 accctgcttc cgcttggttc ggccattgcg ctggcactga tgctggaaac ggacatggga
     2101 aacaacctgc gtgagcgggc tttcagcaca ggaacggcac gtctgcagtc tctgtatctc
     2161 ggtctggatg gacatctggt gcggacggca cgacgcagcc gcgagtttgc cgatctcatc
     2221 gcgtcgtatc cgtttgatca agagctgatt cggcgtgaag cgaaaaggca cgaggatctg
     2281 gacacgtttg cctggtatgc gatcatggat ggctcaaatc gtcttctcat gggcgacatg
     2341 cacatctggc gcccggacat ctacaacctg atggaaaaac tgtgtcggct ggtggtgcgg
     2401 cggtttgtcc cccatcgcgt ggtggagacc gcgggacgtg tcgaaaccat cgaggtgttc
     2461 ctcgaggaag tggcaacgaa cgaacagttg ggatgggccg aagtgctgga aaatcccaat
     2521 gtggtgcaga aacaccagtt gggtggcgcg tcgggcaatt tgtactggac agtgtttcct
     2581 catttgtcga ccggaccggc atttctggcc tgtgcccttg ataccgatcg acttgtacga
     2641 tcgttctttg aaccgatcct ggcgtcgtct ccgtcagccg ctgattttgt gttcggcatc
     2701 catcagcgac actttgagat tgtggccccc gttgcgaaac gggagattcg accgatgctt
     2761 cccctggttc tgaagacgca caagacggcg acaaccgtgc agggagaagt gaagactggt
     2821 acgcgaaggt ggtgggtcgt ggccatgccg gagaattact cgggatacta catccttgcc
     2881 tgggctgccg atgccgacca ggccctgctc gctctgcgtc ccttgcggtg ggccctcttc
     2941 gccggattac tggcaaccct ggccgtggtg ttcggggtct atcggcttct ggtcaacatg
     3001 gttctggcgc ccatcggtga cctgagaacc ggaatcgccg cgattcgaca acggcagacc
     3061 ggggtgcgac tgccgattcg gcgcgctgat gagttcggcg aactggccat gcttttcaac
     3121 cgaactctca gtggactgca ggaactcgaa ctggcgcgac tggtacaaac gcagctgctc
     3181 ccggcgcgga ttccagaagt ggcgggatac accctcgcgg cggaaaacct gacggcgacc
     3241 gaattgagcg gggactacta cgatctggtt cctctgggag acgacaccct gctggccgtg
     3301 atcggcgatg tcacggggca tggcgctccg gccgcattgg ccatggccat ggcgagagcg
     3361 atcatcgccg ctcgtgccgc cgatggagaa aagagtcccg ccgagtactt gcggtcgctg
     3421 aatgaggtgt tctttcagga tatgaagccg acacgtcggt tcatgacctt tctcgccggc
     3481 agactccata tgcgggcaca caagatcgag ttcgcgaatg ccggccacaa ttatccgctc
     3541 gtgtaccggg cggctgccaa atcggtggaa acacccgaga tgccggggct gcctctggga
     3601 attcgacgtc aggtgccacg aacatctcac gagatagatc tactgcccgg cgacgcactc
     3661 gtgtactaca cggatggata taccgaaagc ttgatgccgg atggcacgca ggttggtccg
     3721 gaacggctgg aagccatgtt acaggatgag gcgccgaggg cggcggacgc cgccaggtta
     3781 ttgaaagcac tggtcgagcg gttggatgcc attcgtcgac cgggaccgcg cgacgacgat
     3841 gtcaccatgc tggtgatctc gcgaaaccgc cttcccgcac ctacgtgcgc gagtgagccc
     3901 ggataaacgc gtcgatactg cgatcatagg tctgttcgtt ggctttgggg aacaggcacc
     3961 cggggatctc ggcattgcgc cggtgatagg cgatgcattc gcagcagatt ccctttcgtg
     4021 aacagggttc gtaagtgcaa gtgcacgtct tttcattctt ggacttgttg gcacacgtgg
     4081 tcatggaaag acctccgacg ggatgttgtc gtgatgatac ctcgtcggag aggtgacgcg
     4141 caaacgagtc aggaagcgac ctgcagccat atgtt
//
LOCUS       DAORHD010000278         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22982_length_4173_cov_3.716853, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000278 DAORHD010000000
VERSION     DAORHD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22982_length_4173_cov_3.716853"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /gene="ppk1"
                     /locus_tag="PKO06_07165"
     CDS             complement(<1..1710)
                     /gene="ppk1"
                     /locus_tag="PKO06_07165"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNV69456.1"
                     /translation="MPRQRPSPRPARISETPSPAHAEPITPAVTLPAGKSRRSAQIGP
                     RFFNRELSWLEFNRRVLDEACDRELPLLERLKFLAITASNLDEFFMVRVGGLRRQIGL
                     GLGAKPDPSGMTVAEQLQAIGRAVRRFVTDQYALLADDLEPKLAQAGIHRYPIERLTR
                     DHLDHLERIFDQEIFPVITPMAIASPAEFPLLPGLGLNLIVRLTAPKGAKQSYRFAVI
                     PLPRSLPRFVALPGEIGYQFVLLEEVILLHLERLFPGEPICESIPFRITRNADLTMAE
                     DEVDNFLEEMEEVLDARRQSPCIRLEIDRRCPGSTLSFLKDSLQIQDDQIFHMTGPLH
                     LGAFMALAGMAGFDEYKVESWTPQSVPEFSTGATTFEVIARNDVLLFHPMQSYDPVVQ
                     FIEEAADDPDVLAIKGTLYRTSARSPIVGALARAAACDKHVTALVELKARFDEARNIE
                     WARELERAGVQVIYGIKGLKTHAKCFIVVRREADGIRRYIHFGTGNYNEATARVYCDV
                     SLFTAAEDFGLDASQFFNAITGYSQPLPYRQISAAPLGLRERLLGFIQSEAERSRQGQ
                     EARI"
     gene            complement(1724..3508)
                     /locus_tag="PKO06_07170"
     CDS             complement(1724..3508)
                     /locus_tag="PKO06_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV69457.1"
                     /translation="MPKHQKLPKKGKKKAGKPVRKEQRARKFAAKPAKFGKKTEAKKD
                     GKAKKKQHRRPEQPTLPVQDRQIKPVPPATVSALSAPAAPIRVPLVAVIDVGSTAIRM
                     MIAQPADDGEFHILESLQHPVRLGKDTFTKGRIEGPTIEESVRILKGFKQTLAEYGVP
                     AEEVRAVATSSVREAINRDTFLDRVYIATGIQVEAIEEAEESRLTCLAARHLLPDKSS
                     WDHGHAMIVEVGGGNTDLLLLHDGHVSFSNMYRLGSLRMRETLEASKVPVDRQRKLLE
                     QHIRTNVDVIRRSVPIAQVDTLIAVSGDMRFASSRLKTDPDPSGFFTLTPKAFNSFAE
                     KLALVPVEELVRREHLTYQEAETIGPALMTYRQIAKAFSVKSILVSPTSMREGLLQEM
                     AARGAWSRDFAEQAIHSALSLGVKYQFDERHARHVADLSIKLFRFLREEHRLGQRYEA
                     LLHLAALLHDLGMFVSSRQHHKHSMYLILNSDLFGLTIIDKILISLVARYHRRATPSA
                     EHQEYAVLTRDNRMAVAKLASILRVADALDRNHLQNAREVELRREDGQLSIGIPDLED
                     LTLERQAIKEKGSMFEEVYGLKVVLRRA"
     gene            complement(3513..>4173)
                     /locus_tag="PKO06_07175"
     CDS             complement(3513..>4173)
                     /locus_tag="PKO06_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012912474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV69458.1"
                     /translation="FGCPVFVGNDVDVGTFGEYSFGAGKGGRCVLGVFPGTGIGGGCV
                     YEGNLLRGKTGSILEIGHIQVQRDGPQCGCGRRGCIEAIASRLAIARECAAAAFRGEA
                     PHLYAEAGTDLEKIRSKTLMRAIEEGDTVIERIVRRAARQLGRAIASAVNLLGPDIVV
                     LGGGLVEAMPKLYLAEVESAIRAEAMKAYTSGLKIAAASCGDDAVVLGAAALAARQIK
                     G"
BASE COUNT          820 a         1208 c         1288 g          857 t
ORIGIN      
        1 gatccgcgct tcctgacctt gtcgtgagcg ttcggcctcg ctttgaatga atccgagaag
       61 tctttcgcgc agaccgaggg gagcagcaga aatctggcga tacggtaacg gttgtgaata
      121 gccggtaatc gcgttgaaga actgggacgc atcgagaccg aaatcctcgg cggcggtgaa
      181 cagactgaca tcacagtaca cgcgggcagt ggcctcgttg tagttgccgg tgccgaagtg
      241 aatgtaccgg cgaatgccgt cggcttcgcg ccgtacgacg atgaaacact tggcatgcgt
      301 tttcagaccc ttgatgccgt agatcacctg cactcccgcg cgttcgagtt cgcgtgccca
      361 ctcgatattg cgggcctcgt cgaatcgcgc tttgagctcg accagagcgg taacgtgttt
      421 gtcgcaggcg gccgcccggg caagagcgcc cacgatcggg ctgcgtgccg aggtgcggta
      481 gagcgttccc ttgatagcga gaacgtccgg gtcgtccgct gcttcttcaa tgaattgaac
      541 caccggatca taactctgca tcggatgaaa cagcagtaca tcgtttcgtg cgatcacttc
      601 aaaagttgtg gctcctgtgg aaaactccgg cactgattgc ggagtccagg actcgacctt
      661 gtactcatcg aacccggcca tgccggccag ggccataaaa gcccccaggt gaagcggacc
      721 cgtcatgtga aagatctgat catcctgaat ctgcagcgag tccttgagaa atgagagcgt
      781 ggaaccgggg cagcggcgat cgatttcgag ccgaatgcac gggctctgcc gccgcgcatc
      841 gagcacttct tccatctctt cgagaaagtt gtcgacctcg tcttcggcca tggtgagatc
      901 ggcgttacgt gtgatgcgga acggaatgga ctcgcagatc ggctcaccgg gaaacaggcg
      961 ttcgagatga agcagaatca cttcttcgag cagaacgaac tggtacccga tttcaccagg
     1021 caacgcgaca aatcgcggca gagagcgggg taacggaatc acggcaaacc ggtacgactg
     1081 tttggccccc tttggcgcgg tcaatcgtac gataaggttc aagccgagac cgggaaggag
     1141 cgggaactct gccggcgagg caatcgccat cggagtaatc accggaaaga tctcctggtc
     1201 aaagatgcgc tcgagatggt cgagatggtc gcgagtcaag cgctcaatgg gataccggtg
     1261 aatgcccgcc tgtgccagtt tgggttcgag atcgtcggcc agcagggcat actggtcggt
     1321 cacgaaacga cgcaccgcgc gcccgatcgc ctgcaactgc tctgcgacgg tcatgcctga
     1381 cgggtcgggc ttggcgccca ggcccagccc gatctgtcgt cgcaggccac cgacacgaac
     1441 catgaaaaac tcgtccaggt tcgatgcggt gattgccagg aacttgagcc tttcgagcaa
     1501 gggtagctct cgatcgcatg cctcgtcgag cacgcggcga ttgaattcca gccagctcag
     1561 ctcgcggttg aaaaagcggg gcccgatctg agcggaacga cgggatttgc cggccggcag
     1621 ggtgacggcg ggtgtgatgg gttccgcgtg tgccggggac ggagtctcag aaatgcgagc
     1681 cggtcgtggg gatggacgtt ggcggggcat gggacttctc tccttacgct cggcgcagca
     1741 cgactttgag gccgtagact tcttcgaaca tcgaaccctt ctccttgatc gcctgcctct
     1801 cgagagtcag gtcctcgagg tcgggaatgc cgatcgacag ctgtccgtct tcgcgacgga
     1861 gttcgacctc acgagcgttc tgcaggtggt ttcgatcgag agcatcggct acacgcagaa
     1921 tcgacgccag tttggccacc gccatgcgat tgtcgcgggt caggacggcg tattcctgat
     1981 gctcggccga tggggtcgcc cggcgatggt agcgggccac gagactgatg agaatcttgt
     2041 cgatgatcgt cagcccgaac agatcgctgt tgagaatgag atacatcgag tgtttgtggt
     2101 gctggcggct cgagacgaac atgccgagat cgtgcagcaa cgccgccagg tgcagcaggg
     2161 cttcgtagcg ttgtccgagt cggtgttctt cgcgtagaaa ccgaaacaat ttgatggaaa
     2221 gatcggccac atgccgggcg tgccgctcat cgaactggta cttgacaccc agactcaggg
     2281 ccgagtgaat ggcctgttcg gcgaaatcgc ggctccaggc tccgcgcgcg gccatttcct
     2341 gcaacaggcc ctcgcgcatg ctggtcggcg aaaccagtat ggacttgacg gaaaaggcct
     2401 tggcgatctg tcgataggtc atcagcgccg gcccgatcgt ttcggcttcc tgatacgtca
     2461 ggtgctcgcg gcgcaccagt tcctcgacgg gaacgagtgc cagcttctcc gcaaacgagt
     2521 tgaacgcttt cggggtcagt gtgaaaaaac cggaagggtc aggatcggtt ttcaggcgtg
     2581 acgaggcaaa acgcatatca cctgacacgg cgatcagcgt atcgacctgc gcgatgggca
     2641 ccgatcgtcg tataacatca acatttgttc taatgtgctg ttcaagcaac ttgcgttgtc
     2701 tatcgacggg tactttcgac gcttcgagcg tttcgcgcat gcgcagagag cccagacgat
     2761 acatattcga aaaactgacg tgaccatcgt gcaacaggag cagatcagtg ttcccgccgc
     2821 ccacttcgac gatcatcgca tggccgtggt cccagctcga cttgtcaggt aacagatggc
     2881 gggcggccag acaggtcagg cgactctctt ccgcttcttc gatcgcttcg acctgaatgc
     2941 cggtcgcgat gtagacgcgg tcgaggaacg tgtcgcgatt gatcgcctcg cggacggagc
     3001 tggtcgccac cgcacgaact tcttcggctg gtacgccata ctcagcgagc gtctgtttga
     3061 accctttgag aatgcgcacg gactcctcaa tcgtcgggcc ctcgatgcgt cccttcgtga
     3121 acgtatcttt gcccagacgg acaggatgct ggagagactc gagaatgtga aattcgccgt
     3181 catcagccgg ttgcgcgatc atcatccgga tggcggtgga gccgacgtcg atgacggcga
     3241 cgagcggaac ccggatcggc gccgcgggag ctgaaagcgc tgatacggtg gcaggcggaa
     3301 caggtttgat ctgacgatcc tggacgggca gggtgggctg ttccgggcgg cggtgctgct
     3361 ttttcttggc tttgccgtct tttttcgcct cagtcttttt gccgaacttc gccggcttgg
     3421 ccgcgaactt tcgtgcccgt tgctcttttc tgacgggctt tccagccttc ttcttccctt
     3481 tcttcggcag tttctgatgt ttcggcatgg gcttaccctt tgatctgtcg agcggcgagg
     3541 gcggcggccc ccaagacgac cgcatcgtca ccgcaactcg cggcagcgat cttcaggccg
     3601 ctagtgtagg ctttcatggc ttcggcgcga atggcgctct ccacttcggc aagatacagt
     3661 ttgggcatgg cttcgacgag tccgccgccc aacacgacga tatcaggccc gagcaggttc
     3721 accgcgctgg cgatggctcg gccgagctgt cgggcagcac ggcgcacgat tctctcgatg
     3781 accgtgtcac cttcttcgat tgcgcgcatc agggtttttg agcgaatctt ctcgagatcg
     3841 gtgccagctt cggcatacag atgcggagcc tcgccgcgaa acgcagccgc ggcacactcg
     3901 cgggctatcg ccagccgtga cgcgatggct tcgatgcagc cacgtcggcc gcagccgcac
     3961 tgggggccgt cacgctgcac ctgaatatga ccgatttcca gaatcgagcc ggtcttgccg
     4021 cgcaggaggt tgccctcgta gacgcacccg cctccgatgc cggttcccgg gaaaacgccc
     4081 agcacgcagc gaccgccttt gccagcaccg aacgagtatt ccccgaatgt gccgacgtcg
     4141 acatcgttgc cgacaaagac tgggcatccg aat
//
LOCUS       DAORHD010000279         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_22994_length_4172_cov_3.902356, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000279 DAORHD010000000
VERSION     DAORHD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22994_length_4172_cov_3.902356"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKO06_07180"
     CDS             complement(<1..696)
                     /locus_tag="PKO06_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV69459.1"
                     /translation="MATEKKTVKKAPAKKAPAKKAAVKPAAKKATAKKAPAKKTVKAA
                     KPAKATKKIVKAKPAAKAKAAKKVTPKAKKTVAKKPAKKAAAKKKPAIGKAKKSALKA
                     KKLVAKAKKPAALPKRLPKPWPIARIRNLMDAWGMSQVDFAIFSGVSYDSVTSWCRGR
                     RHLVSRVTSEHLEKAEKIATSRKFKATDGKPWAGFKNYMKNECPVKVDTDLELKSFDG
                     TFKLEVVETAPGKF"
     gene            complement(1517..1879)
                     /locus_tag="PKO06_07185"
     CDS             complement(1517..1879)
                     /locus_tag="PKO06_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV69460.1"
                     /translation="MTINDALSGLPDWTKEFAGAITVCDTTGIILAMNQRACETFTKS
                     GGESLLGKNLLDCHPEPARSKLAGLLAKPTTNAYTIEKNGTRKLIYQAPWSRDGVFAG
                     LVELSLVIPTDMPHFVRK"
     gene            complement(1950..3296)
                     /locus_tag="PKO06_07190"
     CDS             complement(1950..3296)
                     /locus_tag="PKO06_07190"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV69461.1"
                     /translation="MKRVLSIIAVALMVAVFVSPVSAAEKEWTWLVFLNADNNLDEFG
                     VTDQNEMAKIGSNDYLNIVSLIDREKGPATLNYIEKDNIVPVADMGEVDMGDYKFLTQ
                     WVKDMTVKYPAKHYALIIWNHGSGWKNNQNQIIKGISYDDSTGNHITTEQLGLAMAEI
                     KAHLGRNLDILSNDACLMQMMEVAYAVKDSCDFFVASEETEPGDGYPYDGILATLKPG
                     MTAAEFVKPWVAAYSASYNGGTQGNSASTQSAIDLSKLNEVKDAIDGFAKAVIAGKYG
                     TQFKDALTKVQKFYYRTNIDLMHLVQILKPVIKDEGFQTAATKLETALGNAIAANGTT
                     GATMRNAKGLAIYFPATSYSFDAAYAKLAFAADCQWDEMVADYYKKITPPQIIADLEN
                     NDVASLRDYVANSGAQNREVSRDLITKLNFRVFAEGGMPAATQEVVKTLVNELLQK"
     gene            complement(3476..>4172)
                     /locus_tag="PKO06_07195"
     CDS             complement(3476..>4172)
                     /locus_tag="PKO06_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69462.1"
                     /translation="TKTVEQTCIGWVKAFVSSYNGGSQGYEACTQSAIKCSEFPKLCD
                     AINGLAKAIMGGKYTAQINTVLNQVQKFADPDYIDLLHFATLLKAAVKDEPIKTACDK
                     LFAAAKLSIVANANAMSSMKDAMGVSVYFPDSTYSFVAGYKTLDFSKAGLWDEMIEAH
                     LKAKAGDAMTVAVETGDTQTLRDFVANAATHDKAINQFVVETLNFRLHSEGGLSKTLT
                     DEAVSLIKELSAK"
BASE COUNT          825 a         1057 c         1208 g         1082 t
ORIGIN      
        1 gaacttgccg ggagccgttt cgacgacttc cagtttgaag gttccgtcaa agctcttcag
       61 ttcgagatcg gtgtcgacct ttaccggaca ctcgttcttc atgtagttct tgaagccggc
      121 ccagggtttc ccgtcggtgg ctttgaactt gcggctggtg gcgatctttt ccgctttttc
      181 cagatgttcg gaagtgaccc ggcttaccag gtggcggcga ccgcggcacc aggaggtgac
      241 agagtcatag gagacgccgc tgaaaatggc gaagtcgacc tgactcatgc cccaggcgtc
      301 catgaggttg cgaatgcggg cgatgggcca gggcttgggc aggcgcttgg gaagcgctgc
      361 cggcttcttc gctttggcaa ccagtttctt cgcttttagc gcactttttt tcgccttacc
      421 tatcgcaggc tttttcttcg ccgccgcttt cttcgcaggt ttcttcgcga cggtcttctt
      481 ggccttgggc gtgaccttct tggcggcctt ggccttggcg gcgggtttcg ccttcacgat
      541 ctttttggtt gccttcgccg gtttcgcggc tttgacggtc ttcttggcgg gagccttttt
      601 ggcggtggct ttcttcgcgg cgggtttcac cgcagctttc ttcgcgggag ccttcttcgc
      661 cggagctttc ttgaccgtct tcttttcagt agccatcttc gtgttcttcc ccatgtgtaa
      721 aaatttttcg gccgtcgatc cttcacgaat cgacttcaga gatgaaaaaa gtttacacgg
      781 tgcgcgtcaa ctcgtcaagg gtatttgtcc tagttgcgaa aaatatttgt ccgagttgat
      841 agatgtgtga attgtccgag ttgaatgtgg ccgaaatgtg acgcgtcgat tgtcctggtt
      901 gtttggtttg caaaatggct tcaaatgcga ataaaagact gtgtgtgatt ccgagatagg
      961 ttcattgttg tcgacagatg agtgtaccct gtgccgggcg acgtcaatcg tgtctgtctg
     1021 aaaatactcg taccggttgt ctgtcgtctc tcgtgctgat ggtgtgaatg aggcaaggga
     1081 ttggggaagc tgggactgtc gatctggccg gaaacgtgga ataactgtcg actgagtatg
     1141 aacgggatct gctttggccg attcgtgaat ggggcgaaag agcggagggg tggtaatcct
     1201 tctggaggta catgcgaggt cagtgctgag cagacttctc tatccaggtc ggttcaggtg
     1261 tcatatgtcc tgaaaatgat cgtcccagtc gaactgccgg caaacgattg acagttcgtc
     1321 gtgagacaag caggcgcgaa tctgcgagac aacgctgcgc aggacgagcg atgcccgcga
     1381 ggttgtcgaa cccgagtgtg gtcgacccac gcacggtcat tcgcggggtg agactcaaac
     1441 aggttggttg tgaatgctgt atgcggctat gatggtgtgt gtgcgactag tactcttgaa
     1501 atgagtatga agcggatcat tttctcacaa aatgcggcat gtccgtcgga atcaccagag
     1561 acagttccac cagtccggca aagactccat cacgagacca gggagcctga tagatgagtt
     1621 tccgggttcc atttttttcg atggtgtaag cattggtcgt cggttttgcg agcaacccag
     1681 caagctttga gcgcgctggt tctgggtgac aatcgagcag attcttgcca agcagtgatt
     1741 cgccgccgga tttcgtgaag gtctcgcagg ctcgctgatt catggcgaga atgatacccg
     1801 tcgtgtcaca caccgtgatc gcaccagcga attctttcgt ccagtctggt aaaccggaaa
     1861 gagcgtcatt gatcgtcata tgaacttcct tttcatcgaa aatgaaagac cctcgaagac
     1921 cgaagtcttc gagggtcttg atgaacgggt tacttctgca gcagttcgtt gaccagggtc
     1981 ttcacgactt cctgggtggc agcgggcatg ccaccttcag cgaacacgcg gaaattcagc
     2041 ttggtgatca ggtcacggct gacttcgcgg ttctgggccc cggagttagc aacgtaatca
     2101 cgcaggctgg caacatcgtt gttttcgaga tcagcaatga tctgcggagg ggtgatcttc
     2161 ttgtagtagt cggcaaccat ttcgtcccac tggcagtcgg cggcaaaggc cagtttggcg
     2221 taggcggcgt cgaagctgta agaagtggcc gggaagtaga tcgccagacc cttggcgttg
     2281 cgcatggtgg cgccggtggt gccgttggcg gcaatggcgt tccccagagc ggtttcgagc
     2341 ttggtggcgg cggtctggaa cccttcgtcc ttgatgacgg gcttcagaat ctgcaccagg
     2401 tgcatcaggt cgatgttggt gcgatagtag aacttctgga ccttggtcaa agcatccttg
     2461 aactgggtgc cgtatttacc ggcgataacc gctttggcga aaccgtcgat ggcatctttc
     2521 acttcattga gtttcgacag atcgatggcc gactgcgtcg aagcgctgtt tccctgcgtg
     2581 ccaccgttgt aggaagcgga gtaggccgca acccagggct tcacgaattc agccgcggtc
     2641 atgccgggct tcagggtggc gagaatgcca tcataggggt atccgtcgcc gggttcggtt
     2701 tcttcagagg ctacgaagaa atcgcagctg tctttcaccg cgtaggcgac ttccatcatc
     2761 tgcatcaagc aggcgtcatt ggaaaggatg tcgagattgc ggcccagatg agccttgatc
     2821 tcggccatgg ccaagcccag ctgttcggtg gtgatgtggt tgcccgtcga atcatcgtag
     2881 gagatgccct tgatgatctg attctggttg ttcttccagc ccgaaccgtg gttccagatg
     2941 atcagagcgt agtgcttggc gggatacttc acggtcatat ccttgaccca ctgggtcagg
     3001 aacttgtagt cgcccatatc gacttcgccc atgtcggcga ccggaacgat attgtctttc
     3061 tcaatgtaat tcagggtggc agggcccttt tcgcgatcga tcaggctgac gatattcaaa
     3121 tagtcgttcg agccgatctt ggccatttcg ttctggtcgg tgacgccgaa ttcatcgagg
     3181 ttgttgtcgg cattcaggaa gaccagccag gtccattctt tttcagcggc gctcacggga
     3241 gagacaaaaa ccgcgaccat aagagcaaca gcaataatac tcagtactcg cttcatccta
     3301 catactcctt atgatactta tagacccgct tgaactgggc ctgtcatacc ttatacgaaa
     3361 ccaacacaga tacactcaat aatcagttgc agagatcgta acacaccgtt tgtacagatg
     3421 caagtcagtt ctggaatacc ggagccgtgt cgccacgact ccggatagtc tcgtcttatt
     3481 tcgccgaaag ttccttgatc aggctgacgg cttcgtcggt cagggttttg ctcaggccac
     3541 cttcggaatg caggcggaag ttcaaggtct cgaccacaaa ctgattgatc gctttgtcgt
     3601 gagtcgcggc gttggccacg aaatcgcgca gagtctgtgt gtcaccggtt tcaacggcga
     3661 cggtcatggc atcaccggct ttcgccttca gatgtgcttc gatcatttca tcccacagac
     3721 cggctttgct gaaatcaagc gtcttatacc cggcgacgaa cgaatacgtg gaatccggga
     3781 agtagacgga aacgcccatc gcgtctttca tgctgctcat ggcattggca ttcgctacga
     3841 tgctcagctt ggcagcggca aacagcttgt cacacgcggt tttgatcggc tcatccttca
     3901 cggcggcttt cagcagcgtc gcgaagtgca gcaggtcgat gtaatcgggg tcggcgaact
     3961 tctgcacctg attcagcacg gtgttgatct gagcggtgta tttgccgccc atgatggctt
     4021 tggcaagacc gttgatcgcg tcgcacagct tggggaattc gctgcacttg atcgccgact
     4081 gcgtgcaggc ttcgtatccc tggctgccgc cattgtagga actgacgaat gccttcaccc
     4141 agccgatgca ggtctgttcc acggtcttgg tg
//
LOCUS       DAORHD010000280         4169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23017_length_4169_cov_4.023335, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000280 DAORHD010000000
VERSION     DAORHD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23017_length_4169_cov_4.023335"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PKO06_07200"
     CDS             <1..935
                     /locus_tag="PKO06_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV69463.1"
                     /translation="RVKNQFIEFRVAILPSIYGQNCTIRVLDSGRVNLDLKTLGFHEN
                     ELKVFEHNIHKPWGLCLIAGPTGSGKTTTLYSAISLINSRQKKIITIEDPVEYKIPGV
                     HQMQVFENKIDEGRSLTFARGLRSILRLDPDIILVGEVRDYETAEITVKAALTGHMVF
                     STIHANSAIESITRLENLGIDPYLFASSLALLVGQRLVRKICPDCKAAISNTPYLLAS
                     MGLDPVQFKDQQFYKGQGCKSCNNSGYKDRTGLFEVIEVTPELKEIISQRASAVALIK
                     HMRAHKVQTIIDSCLSKVLLGEVPVEEFISVNMQ"
     gene            948..2012
                     /gene="pilM"
                     /locus_tag="PKO06_07205"
     CDS             948..2012
                     /gene="pilM"
                     /locus_tag="PKO06_07205"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNV69464.1"
                     /translation="MPRAPAFPLVLDLGTHNMRLLKLRSASAGEIDLQKLAVIDSPRE
                     FAVSTYIESPIMDPQAVEEALKGLLKNVKAGFENALVLLPDHSALINLMVGPARYSRK
                     EQEEAVKEDMTPIMPLPPELWHIVFESIGRWEEDEITLALATIKSNLLEIGGLVQKAG
                     FNPQTVDVNFFNVSNLIEHYLTDEGNRGKNIALVHLGHETTSIGVFKDGALRSFLNRP
                     IGSFDFTKQISKHFHVPESEAEHFKRTEIFFLPEPSPEQDETYNYTVIKSVFAALLRE
                     IFSVLEAYLTKFREFSIQEVVISGGGSNFQNISVILSSNLNAPVRQVGELYRLTTDGA
                     EVGIPEKNALAAACGAFLRG"
     gene            2016..2579
                     /locus_tag="PKO06_07210"
     CDS             2016..2579
                     /locus_tag="PKO06_07210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNV69465.1"
                     /translation="MKFQFDLLPMEYKSLPRDNVGIMLAIVALVLCVSAITSLNFKNK
                     KEMAARQSEIDAAQAQLSQTYQQAAAIQPPVDRIENLKRSIQFINSNMETPGTSCVDF
                     LTALEANVPEKVFIRDVNPKDFSGRGTVFTIDGEAATIYDVLDLISRLQKSETFRDVF
                     LKNNSTRAVEGGIVTAFSLTCVYAGSK"
     gene            2602..3183
                     /locus_tag="PKO06_07215"
     CDS             2602..3183
                     /locus_tag="PKO06_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69466.1"
                     /translation="MKTRMRSILERVISYGLFAAAVGGFFFFYTNVNKAQKETIEQMR
                     KKVLELQDLQVKADEDLKSRQRQIETVNAQIEKRRLEVKERFERLLEKAENYTHFIEQ
                     VQRKARSLDIMILTSQYEPPARAVNAPEDYQEFKFTLDVKGAYEKMKRFLWEIENALG
                     RFVKISKLVIKPPICDADGNMSLSLTLSTFFLK"
     gene            3186..3866
                     /locus_tag="PKO06_07220"
     CDS             3186..3866
                     /locus_tag="PKO06_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69467.1"
                     /translation="MTQEEFKTLIQKNKAAAGGILFAALIFVTDVFYLKPAREARRKA
                     LLQGTQTPSATAAPDAGAALPTDPGAGSAMPVPPPELFINLGEIPPLNQKVESRFSVK
                     TDYPYPLRRNVFYTAKKEEPILEPTPVYTEEVATPTSRPDITYHGFYNVGPDKIAIMR
                     LAQRLLLARMGQRLDESPFVLELILGDRVLIRDLEHADDQFEVNLAEATPAKAGPGSG
                     SGGQLQGR"
     gene            3870..>4169
                     /locus_tag="PKO06_07225"
     CDS             3870..>4169
                     /locus_tag="PKO06_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69468.1"
                     /translation="MDYATLKNLFRDRDKMIRLFCLKTAVKEKLPQIGEFVIDGLRDE
                     RPEVVTAALKGARLCEDPEIIGMVLTYLESPNTMLRSEALQALEGKRHPMVREALG"
BASE COUNT          967 a         1142 c         1162 g          898 t
ORIGIN      
        1 tgcgggtgaa gaaccagttc atcgagtttc gcgtcgcgat tctgccgtcg atctatgggc
       61 agaactgtac gatccgcgtt ctcgactcgg ggcgtgtcaa tctcgatctc aagacgctcg
      121 gattccacga aaacgaactc aaggtttttg aacacaacat tcacaagccc tgggggttgt
      181 gtctgatcgc cggaccgacg gggtctggga agacgacgac gctctactcg gcgatttcgc
      241 tgatcaactc gcgtcagaag aagatcatca ccattgaaga cccggtggaa tacaagattc
      301 ccggggtgca tcagatgcag gtctttgaga acaagatcga cgaggggcga tccctcacct
      361 ttgcccgcgg tctccggtcg attctgcgtc tcgaccccga catcattctc gtcggcgaag
      421 tccgcgacta tgagacggcc gaaatcacgg ttaaggccgc gttgaccggt cacatggtct
      481 tttcgacgat tcacgccaac agcgccatcg agtcgatcac ccgcctcgaa aacctgggca
      541 ttgaccccta tctctttgcc tcatctctcg cacttctggt tggacaacga ctcgtacgta
      601 aaatctgccc agactgtaaa gccgccattt cgaatacccc gtatctgctg gccagcatgg
      661 gccttgatcc ggtgcagttc aaggatcagc agttctacaa gggacagggg tgcaagagct
      721 gtaacaacag cggctacaag gatcgtaccg gcctgttcga ggtcattgaa gtgacgcccg
      781 agctgaagga gatcatcagc cagcgcgcat cggcggtggc gctgatcaag catatgcgtg
      841 ctcacaaggt gcagaccatc attgattcgt gtctttcgaa agttctgctc ggagaggttc
      901 cggtggaaga gttcatttcg gtcaacatgc agtagcgagg aggagcgatg ccacgcgcac
      961 ccgcatttcc attagtcctg gacctgggga cgcacaatat gcgtctgctc aagctccgca
     1021 gcgcctcagc cggtgagatc gatctgcaga agctggcggt gatcgattcc ccccgcgagt
     1081 tcgcggtgtc gacctatatc gaatccccga tcatggatcc gcaggcggtc gaggaagcct
     1141 tgaagggctt gctcaagaat gtgaaggccg gtttcgagaa tgccctggtt cttttgcctg
     1201 atcattcggc tttgatcaat ctgatggtcg ggcctgcccg gtactctcgg aaagagcagg
     1261 aagaagcggt gaaggaagac atgaccccga tcatgccgtt accacctgaa ctgtggcaca
     1321 tcgtcttcga aagcattggg cgctgggaag aagatgaaat cacgcttgcc ctggctacca
     1381 tcaaatcgaa tctgctcgag atcggggggt tggtgcagaa agcgggtttc aatcctcaga
     1441 cggtcgatgt caattttttc aacgtctcga acctgatcga acactatctg accgacgagg
     1501 ggaaccgggg caagaatatc gccctggttc atcttgggca tgaaaccacg tcgatcgggg
     1561 tgttcaagga tggggcgctg cgatcgtttc tcaatcgacc gatcgggtcg ttcgacttca
     1621 ccaagcagat cagcaaacat tttcacgtgc cggaaagcga agccgagcac ttcaagcgca
     1681 ccgagatctt tttcctgccc gagccgtcac cggaacagga tgaaacctat aactacaccg
     1741 tcatcaaatc tgtcttcgcc gcattgctgc gcgaaatctt cagtgttctc gaggcctatc
     1801 tcaccaagtt ccgcgaattc agtattcagg aggtcgtgat ctccggtggt ggaagcaact
     1861 tccagaacat ctccgtcatt ctctcctcca atctgaatgc gccggtgcgg caggtggggg
     1921 aactctatcg tctgaccacc gatggtgccg aggtcggaat tccggaaaag aacgcgctgg
     1981 cggccgcatg cggtgcgttt ttgcgggggt agctcatgaa attccaattc gaccttctgc
     2041 cgatggagta caagtccctg ccacgggaca acgttggtat catgctggca atcgtggctc
     2101 tggttctctg cgtgtcggcc attacctcgc tcaacttcaa gaacaagaaa gagatggcgg
     2161 cccggcaaag cgaaatcgac gcggcccagg ctcaactcag ccagacctat cagcaggctg
     2221 ctgccattca gccgcctgtc gatcgcattg aaaatctcaa acgcagcatt cagtttatca
     2281 acagcaacat ggaaacgccg ggaacgtcat gtgtcgattt tctgaccgcg ctcgaagcca
     2341 atgttcccga gaaggtgttc attcgcgatg tcaatccgaa agacttttcc ggtcgcggaa
     2401 ccgtcttcac catcgatggt gaggcggcga cgatctatga tgtgctcgat ctgatctcgc
     2461 gtttgcagaa gtcggagacg tttcgtgacg tgtttctgaa gaacaattcg acacgagccg
     2521 tcgaaggtgg catcgtcacc gcattttccc tgacctgcgt gtacgcaggc agcaagtaga
     2581 tcgcagagag gagacgcact cgtgaaaacc cgcatgcgat cgattctgga acgggtcatc
     2641 agctatggcc tgtttgccgc ggccgtgggc ggctttttct ttttctatac caacgtcaac
     2701 aaggcgcaga aagagacgat cgagcagatg cgcaagaagg tgctcgagct tcaggatctg
     2761 caggtcaagg ctgacgaaga cctcaagtcg cggcagcggc agatcgagac cgtgaacgcc
     2821 cagatcgaaa agcggcgact cgaggtgaag gaacgatttg aacgtctgct tgaaaaggcc
     2881 gagaactata cgcatttcat cgaacaggtg cagcgcaagg caagatcgct cgacatcatg
     2941 attctgacct ctcaatatga gccgcccgcg cgtgcggtca atgctccgga ggattatcag
     3001 gagttcaaat tcacgctcga cgtcaaaggg gcctacgaaa agatgaagcg atttctctgg
     3061 gaaatcgaga atgcgctcgg tcgttttgtg aaaatcagca aactggtcat aaagccgccg
     3121 atctgtgatg ccgatggaaa tatgagcctc agtctgacat tgtcgacttt ctttctcaag
     3181 taagcgtgac ccaagaagaa ttcaaaactc tgatccagaa aaacaaggct gccgctggcg
     3241 gtatcttgtt tgccgcgctc attttcgtaa ccgatgtttt ctatctgaaa ccggcacgtg
     3301 aagcgaggcg taaagcactg cttcagggaa cgcaaacgcc ttctgcgacg gcagctccag
     3361 acgcgggtgc cgctctcccc acggatccag gcgcaggctc cgcgatgccg gttccgccac
     3421 ctgagctatt catcaacctt ggcgagattc cccccttgaa ccagaaggtg gaaagtcgct
     3481 tttcggtgaa aaccgattat ccgtatccct tgcgtcgcaa tgtgttctac accgcgaaga
     3541 aggaagagcc gattctcgag ccgacgccgg tatacacgga agaggtcgcc actccgacct
     3601 ccagaccgga catcacgtat catggcttct acaacgtcgg accggacaag atcgccatca
     3661 tgcgtctggc gcagcgcctg ctcctggcac gaatgggaca acgtctggac gagagcccgt
     3721 ttgtcctgga actgattctc ggagatcggg tgctgatacg cgatctcgaa cacgcggacg
     3781 accagttcga agtgaatctg gcagaagcca cgccggcgaa ggcgggcccg ggctcggggt
     3841 ctggtggcca gctacaaggc aggtgacgca tggattacgc aaccctgaaa aatctgttcc
     3901 gcgatcgcga caagatgatt cggctcttct gtctgaaaac cgccgtcaaa gagaagcttc
     3961 ctcagatcgg tgaatttgtc atcgacggtt tgcgtgatga acgcccggaa gtcgtgaccg
     4021 cggctttgaa aggcgctcgc ctttgcgaag accccgagat catcggcatg gtgcttacct
     4081 atctcgaatc gccgaatacg atgctgcgtt cagaggcgct gcaggcgctc gaaggcaagc
     4141 gtcatcccat ggtgcgtgag gcgctcggt
//
LOCUS       DAORHD010000281         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23044_length_4166_cov_3.753831, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000281 DAORHD010000000
VERSION     DAORHD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23044_length_4166_cov_3.753831"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKO06_07230"
     CDS             complement(<1..294)
                     /locus_tag="PKO06_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69469.1"
                     /translation="MMMLTPEAGNRIFRRSGMLELLLAFLAVVAMPATGLELQQPKLA
                     VTSLECKVSPRDLPSDAVERPFQWFVDGALATEGRAVPTALAPLTHTFSTSGRH"
     gene            572..1498
                     /locus_tag="PKO06_07235"
     CDS             572..1498
                     /locus_tag="PKO06_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV69470.1"
                     /translation="MIGDRSCRFSFLFLLMLALLLTSSADARPRVAVLEFTNPGGFDS
                     SYPIGEGMSYLLRKRLMDAGKVQVVDRADLESLKTELRLSEDGFFDSSTFPARGGFQG
                     ADYIVRGKVLDFGHFSRDTGIGALGKIAGEKLAGGFTQTKTVAHVRISVEVIDLRTGR
                     LAMSDNSEARHEKTGTILLAGDLKTAIGAAIKLGDSKFDASMIGKASGKVLDKLAVRI
                     NGLFNMEARVLAVSPDGTVIDMGTSSGLTVGKRGKLYTLKEIKNAKGMVVWCSKTLAG
                     DVTVTEVQPEGALVSPIPGTSPKEGDLVVFDH"
     gene            1514..1933
                     /locus_tag="PKO06_07240"
     CDS             1514..1933
                     /locus_tag="PKO06_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69471.1"
                     /translation="MRLFLYLVFAVILCPSVVSAATASEPIAPPPDQTASSTPATETP
                     SWQPGRHLIRRMSLPHCKRGPMRCAKCKAGEGQIVFSLIDVFPPNRGMVQRPVTEVTI
                     DGTKQYLEYDVVKVFATEAEARAFAAQHQLTDCQWSE"
     gene            2016..>3027
                     /locus_tag="PKO06_07245"
     CDS             2016..>3027
                     /locus_tag="PKO06_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV69472.1"
                     /translation="MKRHMHAILGGVLFAVTAVGFAQVDLSTTELIKVAGKVEVQKGQ
                     DPAYKAVPTNLKLAGALKRLDAGDKVKTHDQSGAEMVLKDTCILGVKEKSQFEVPSTL
                     GAGSLAKLKAQQGSFLFKVVSGSNFKVQTSEVIAGVKGTLFGIEVTDNLAPFLKLPGL
                     DLGLENPGGTMVNVYEGEVELNHAVTGKRRTVKAGERLAVLNRVLSGLDKSLGEGFSP
                     IEKIDILKDLAANFGEAGLSKLRVSSKIEGLKQLGIEGYSLLTGTGDRKQQLQMIKQQ
                     LPAAAQERWSKFEQIRGAWRGLRSELQNLKGKPFVPTFDEGKYPTRPTAKLIPENGIE
                     EAH"
     assembly_gap    3028..3037
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3038..3545
                     /locus_tag="PKO06_07250"
     CDS             <3038..3545
                     /locus_tag="PKO06_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69473.1"
                     /translation="HLGQGLFMAMAPNPGCQVLKLEPEEAAYVVKEGEGSFRIQDFAH
                     DLAGQVIIRSDQGKLITTATMSKGTLYTRIPGDATTIAIQPGKPVEIEFDPTAGTSRF
                     RPSPASVALPQQLAEYRFQVETDLDAQKAAHQQQVNENRSNAVQNLLKNAPKGKDLRK
                     GFRNLFGK"
     gene            3657..>4166
                     /locus_tag="PKO06_07255"
     CDS             3657..>4166
                     /locus_tag="PKO06_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV69474.1"
                     /translation="MKHLRERLLLAVPVIICLLTLVPVGNRIVAWLDQKYFDILFTFS
                     HPPAVEHPLSLVVKDQSFQERFGRDPDRRDMARIVSLLNRAGVRTAGFDLIYDQPQNE
                     ETDQILATAMSRFGGAILAQRFSSRAIQAGLDTITLTTGGRPPAPLALYAPISDAAFG
                     TGIINILAGM"
BASE COUNT          918 a         1192 c         1196 g          850 t
ORIGIN      
        1 atgcctgccc gaagtcgaga acgtatgggt gagaggcgcg agtgccgtcg gaacagctcg
       61 gccttccgtc gccagagcac cgtcgacaaa ccattgaaac ggtcgttcaa ccgcatccga
      121 aggcaggtcc cgggggctga ccttgcattc gaggctcgtc actgccagtt tgggctgctg
      181 cagttcaaga cctgtcgcgg gcatcgccac gacggccagg aatgccaaca ggagctcaag
      241 catgccgctt ctgcggaaga tccggttccc agcttcaggt gtcagcatca tcacgattcc
      301 tctccatcac gaaattgaac gtgtcacact agcatgtatc gaatggttcc tcaacccggc
      361 acaatcacgt gggggcggtt ctcagcaggt atgacgcgac agagtgatcc atgcccaggc
      421 tctcgctgac cgttcatgcg gtgactcgtc gtgaaggcga tgatagcggc atgattgaca
      481 agatgggaac cacacgggat gaatgtagac gccatcgaaa cggacggtgt acaatctcgg
      541 cgaacaagaa gaccacttcg ggaggccgtt catgattggc gatcgttcct gtcgcttttc
      601 gttcctcttt ctgctcatgc ttgctctgct tctcacgagc agcgctgatg cgcgcccgcg
      661 agtcgccgtg ctcgagttca cgaatcccgg cggtttcgac agcagttacc cgattgggga
      721 agggatgtcg tatctgctgc gcaagcggtt gatggatgcc ggcaaagttc aggtcgtcga
      781 tcgagccgat ctcgaaagcc tcaaaacgga gctgcgcctt tccgaagacg gcttctttga
      841 ttcgtcgacg tttccagccc gagggggttt tcagggtgct gactacatcg tgcgcggcaa
      901 ggttctcgat ttcggtcatt tctcgcgcga taccggcatc ggcgcgttgg gcaaaattgc
      961 cggagagaaa cttgccggtg gttttaccca gaccaaaacc gtcgcccacg tacgcatcag
     1021 cgtcgaggtg atcgatttgc gtaccggtcg tctcgcgatg tcagacaatt cagaagctcg
     1081 tcacgagaaa accggaacga tacttctcgc cggcgatctg aaaacggcaa tcggtgccgc
     1141 catcaagctg ggtgactcga agtttgacgc gtcgatgatc ggcaaagcct cgggcaaggt
     1201 gctggataag ctggccgttc gaatcaatgg gttgttcaac atggaagcac gcgttctggc
     1261 agtcagcccg gatggcacgg tcatcgacat gggcacgtcg tcgggtctga cggttggtaa
     1321 acgcggaaaa ctctacacgc tcaaagagat caagaatgca aagggcatgg tcgtctggtg
     1381 ttccaagaca ctggccggcg acgtcacggt gaccgaggtt caacccgagg gcgctcttgt
     1441 gtcacccatt cccggcactt cacccaaaga gggtgatctc gtcgtgttcg atcactgagg
     1501 ggggcccttc caaatgcgat tattcctgta tctcgttttc gcggtcattc tctgtcccag
     1561 tgttgtttcg gcggcgacag cttctgagcc gatcgctccc ccgcccgacc agacagcttc
     1621 ctcgacaccc gccacggaaa ctccctcgtg gcaaccgggg cgacacctga ttcgacgtat
     1681 gtcgcttccg cactgtaagc gtgggcccat gcgctgcgcc aagtgcaaag ccggcgaggg
     1741 gcagatcgtg ttcagtctga tcgacgtgtt tccccccaat cgtgggatgg tgcagcgccc
     1801 cgtcaccgaa gtgacaattg atggcacgaa gcagtatctc gaatacgacg tcgtcaaagt
     1861 gtttgccacc gaggcagagg cacgcgcgtt tgccgcgcag catcagctca ccgattgcca
     1921 atggtccgag tgagccaact cgcgcctttg acggcgagtg ccaatatcag tatgatgccc
     1981 gcaccccgga acacccggaa tgccaaggag ttgatatgaa acgtcacatg cacgccattc
     2041 ttggcggcgt tctgttcgcc gtcacagccg ttggttttgc ccaggtcgac ctgagtacta
     2101 ccgaactgat caaggtcgcc gggaaggtcg aggtccagaa gggccaggat cccgcctaca
     2161 aggcagtccc gacaaatctc aaacttgccg gagccctgaa aagactggac gccggagaca
     2221 aggtcaagac gcacgatcag agtggtgcgg aaatggtgtt gaaggacacc tgtattctgg
     2281 gcgtcaagga aaaaagtcaa tttgaagtac catccactct cggagctggc tcgctcgcca
     2341 agctgaaagc acaacaaggt tcttttcttt ttaaggtcgt cagcggcagc aacttcaagg
     2401 tgcagacttc cgaagtcatc gcgggcgtca agggaacgct gttcggtatt gaagtcaccg
     2461 acaacctcgc cccgtttctc aaactccccg gacttgatct ggggctcgag aatccgggcg
     2521 gcacgatggt caatgtctat gagggcgaag tcgaactgaa ccatgccgta acgggaaaac
     2581 gtcgaaccgt taaagcagga gaacgtcttg cggtgctcaa ccgagttctt tctggcctgg
     2641 acaaatcgct gggtgaaggt ttcagtccga ttgagaaaat cgacatattg aaagatctgg
     2701 cggcgaattt cggagaggct ggtctgagca agctcagagt ttcttcaaaa attgaggggc
     2761 tcaagcaact cggaatcgaa ggttacagct tgttgacggg aactggagat cgcaaacagc
     2821 agctccagat gatcaagcag caactccccg cggctgcgca ggaaagatgg agcaagttcg
     2881 agcagatcag gggtgcttgg cgaggtctgc gttcggagct gcagaatctg aagggcaagc
     2941 cctttgttcc aaccttcgac gaggggaagt acccgactcg tcccacagcc aagctgattc
     3001 cagaaaacgg gatcgaagaa gcccaccnnn nnnnnnncca cctcggacag ggtctgttca
     3061 tggccatggc gcccaacccc ggctgtcagg tgctgaaact tgagccagaa gaagcggcct
     3121 acgtcgtgaa ggaaggggaa ggatcatttc gcattcagga cttcgcacat gacctggccg
     3181 gtcaggtgat cattcgcagc gatcagggca aactcatcac cacggcgacc atgagcaaag
     3241 gcacgctcta cacacggatt cccggcgatg ccacgacgat cgccattcaa ccgggcaagc
     3301 cggtcgagat cgagttcgac ccaacagccg gaacgtcacg cttccgtccc tcgcctgcct
     3361 cggtcgctct gccgcagcag ctggcggaat atcggtttca ggtcgagacg gatctcgacg
     3421 cccagaaagc ggcgcatcag cagcaggtca acgaaaatcg cagcaacgcc gtgcagaacc
     3481 tgctgaagaa cgctcccaag ggcaaggatc tccgcaaggg atttcggaat ctcttcggga
     3541 aatagctgtt catcaccgtt ttccagcccc cacctgcgtg ggggtttttc attcctgtcg
     3601 ctctgaggcg gcgacaaacg gcagcatgcc gccgatgatt ctgctatcat tctggcgtga
     3661 aacatctgcg tgaacgtctg ctgctggcgg tgccggtgat catctgcctg ctcaccctgg
     3721 tgccggtggg caatcggatt gttgcctggc tcgaccagaa gtatttcgac attctgttca
     3781 ccttcagtca ccccccggct gtcgagcacc cattgtcact ggtggtcaag gaccagagtt
     3841 tccaggaacg cttcggacgc gatccggacc gccgcgacat ggcacgcatc gtctcgctgc
     3901 tcaatcgtgc gggtgttcga accgccgggt tcgacctgat ctacgaccaa cctcagaatg
     3961 aagagacaga tcagatactc gctacagcca tgtcccggtt cggtggcgcc attctcgctc
     4021 agcgtttttc ttctcgcgcg attcaagcgg gactcgacac gatcacgctg acaacggggg
     4081 gaagaccgcc ggcgccactt gccctctacg ctccgatctc agacgccgcg ttcggcacag
     4141 gaatcatcaa cattcttgcg ggcatg
//
LOCUS       DAORHD010000282         4161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23086_length_4161_cov_3.659279, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000282 DAORHD010000000
VERSION     DAORHD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23086_length_4161_cov_3.659279"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /gene="yhbY"
                     /locus_tag="PKO06_07260"
     CDS             complement(<1..455)
                     /gene="yhbY"
                     /locus_tag="PKO06_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNV69475.1"
                     /translation="MNLTSKQRKHLESLAHELDPIVRIGKNGVTPTLIRTADEALQAR
                     ELIKVKLLDNAALDKHEVAAEIVAATEATLVRVIGNIIVLYRQNLKRKYHIQLDGQPQ
                     PEATPVKKPLKRARKPAPRRTSRPMAARDDSPRPPRASPWAKAKGPRQVP"
     gene            complement(559..3741)
                     /locus_tag="PKO06_07265"
     CDS             complement(559..3741)
                     /locus_tag="PKO06_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69476.1"
                     /translation="MRYIRWCINNPVSVNLLVLFLIISGGISLYSMKREIFPSFSLDR
                     VLITVVFPGASPEEVEEGVCIKIEEALTGLAGIKKVSATARESTGIVIAEIETGEDID
                     ELKNEIETRINSINTFPRDAEKPVIKKLELIRQTLQLVVSGALTDEAMRELAEEIRRD
                     LLALPEISQIMLDGLKDHEVSIEVSEATLREFGLSFDDVARAIRTNSLDLSGGVVRTP
                     GGEVLLRVKGQKYIRREFEDLVIRTASSGATIRLSQIAHVRDGFADGQIEASLNGEKA
                     CMVSVFKTEEEDALVIADTVREYAERKRLELPPAVKLTILNDTSVAVRSRLDLLSSNG
                     LQGLILVFVCLWLFMDLKLSLWVSFGIPVSMLGSFLLLGIMDQTLNMMSMFGLIMALG
                     MIVDDAIVISESTYSQLEKNPFQNPARAALYGTARVFWPVLASVTTTIVAFLPLLAIT
                     GIMGKFIAVLPITVISCLLMSLVEALTSLPCHLAHSLTAPKPEQPHSRLRDLVDRGFR
                     RIIAVYARLLRVACAFRYATVGLAIFILLTSIGIVRAGWVPFVLFARNDSDTVIARVV
                     FPEGTSFEATRGAIIRLKAAISQTNQWLKTTYQLKTDAVRHSFALIGQHSGNEPTFGT
                     NRGELLVELLPAEERTFNSGVVLQKWQEFTGPMPEARSLVFVERETGPGGRPIEVQLR
                     STSFDKLRSASKEMREHLTTFPGVYNIEDDFKEGATELRFDLKDGARHLGLSLADLAG
                     QLRHGFYGNEALRLQRGRFDVKVFVRYPEQDRRYLSTLLSMTIRGPNGAEIPLSDVAN
                     IREHAGTAEIKRVEGQRVISVLADVDERKANAAAITTNLVSSGYLDLLCRKHGISYAF
                     EGQRKETMESLAGLRVGFALSFLGIYIILATIFASYLQPIIIMVAIPFGIIGALIGHW
                     IMGYPLTMMSMFGLVALSGIVVNNSLLIVDYINEAVGQGTPLADALILAGSERFRPIM
                     STSLTTFFGVSSLLFERSFQAQFLIPMAITLAWGLIFSTLLSLILIPALYAILVDGLR
                     LMSRLRYGQWPSDAELLLTPEALPVEPGES"
     gene            complement(3752..>4161)
                     /locus_tag="PKO06_07270"
     CDS             complement(3752..>4161)
                     /locus_tag="PKO06_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69477.1"
                     /translation="KSSQTRSTGAITRVAPSLADKTRTLELVVAVSPNFSASSSLFLP
                     LLRGMFVEVSLLGRPLQQVYKVPRALVSREDTIPLYKDGRLLLRQVETLRLDGDEAFI
                     RAPLASDEQLVVTPLARAVEGMRLSIASSAEVP"
BASE COUNT          857 a         1157 c         1298 g          849 t
ORIGIN      
        1 ggtacttgcc gaggaccttt ggctttcgcc cacggagagg ctcttggcgg acgaggactg
       61 tcgtctcttg cagccatcgg gcgcgaggtt cggcgaggcg cgggcttgcg tgcccgcttt
      121 aacggcttct ttacgggggt ggcttccggc tgtggttgtc catcgagctg aatgtgatac
      181 ttgcgcttca gattctggcg atagagcacg atgatgttgc cgatgacgcg taccagcgtc
      241 gcctcggtcg ccgccacgat ttcggcggcc acttcgtgct tgtcgagcgc ggcattgtcg
      301 agcagtttga ccttgatcag ctcgcgggcc tgcagggcct catcggccgt gcggatcagc
      361 gtcggcgtga ccccgttctt cccgatgcgt acgatcgggt cgagttcgtg ggccagcgat
      421 tcgagatgct tgcgttgttt cgatgtcagg ttcatgcgat ctccttcgag cgccaccggg
      481 gttgtgactt cacccttggc ggcactccgg tttgtgggtg cgctccgtat gataacaggt
      541 ttttcgatcc tgtccagatc agctctctcc cggctcgacc ggcagggctt caggagtcag
      601 cagcagctcg gcgtcgctcg gccattgccc gtatcgcagg cgtgacatga gccgcaggcc
      661 atcgacgaga atggcataca gcgccggaat gaggatcaga gacagcaacg tgctgaaaat
      721 cagcccccag gccagagtga tcgccatcgg aatcaggaac tgtgcctgaa aactgcgctc
      781 gaagagcagt gacgagaccc cgaagaaggt ggtcagtgac gtggacatga tggggcggaa
      841 tcgctcagag ccggccagaa tcaatgcgtc ggccagagga gtgccttgtc cgaccgcttc
      901 gttgatgtaa tcgacgatca gcagcgagtt gttaacgacg atgccgctga gtgcgaccag
      961 accgaacata ctcatcatcg tgagcggata gcccatgatc cagtgaccga tcagggctcc
     1021 gataatgccg aatggaattg cgaccatgat gatgatgggt tgcagatagg aagcaaagat
     1081 ggtggcgaga atgatgtaga tgccgagaaa cgagagggcg aagccgacgc gcagccccgc
     1141 cagtgattcc atggtttcct tgcgctggcc ctcgaaagca tacgagatgc cgtgctttcg
     1201 acagagaagg tcgagatagc cgctcgagac caggttggtc gtgatggcgg cggcattggc
     1261 cttgcgctca tccacgtcgg cgagtaccga gatgactcgc tggccctcca cgcgcttgat
     1321 ttcggctgtt cccgcatgtt cgcggatgtt cgcgacgtcc gagaggggaa tctcggctcc
     1381 attcgggcca cgaatcgtca tcgagagcaa ggttgacaga taccgtcggt cctgctcggg
     1441 atagcgaacg aacactttca cgtcgaagcg tccgcgctgc agtcgtagag cttcgttgcc
     1501 gtaaaaaccg tgtcgcagtt ggccggccag atcggcgagg ctgagtccga ggtgacgggc
     1561 tccgtccttc agatcgaatc ggagttcggt cgctccctct ttgaaatcgt cctcgatgtt
     1621 gtaaaccccc ggaaatgttg tcagatgctc tcgcatctct tttgatgcac tacgcagctt
     1681 gtcgaaactt gtactacgaa gttgcacttc gatggggcga ccgcccggcc ccgtttcgcg
     1741 ttcgacgaag accaggctgc gagcttcggg catggggccg gtgaactcct gccacttctg
     1801 cagcacgaca ccagaattga aggtcctctc ctcagccggg agcagctcga ccaagagctc
     1861 gccacggttg gtgccgaacg tgggttcgtt gcccgagtgc tgaccgatga gggcgaacga
     1921 gtgccgaacg gcatcagttt tcagctgata cgtggttttg agccactggt tggtctggct
     1981 gatagccgct ttcaagcgta tgatcgcgcc gcgggtggct tcgaacgagg tgccttccgg
     2041 aaaaaccacg cgggcgataa ccgtgtcgga atcattgcga gcaaacagga caaatgggac
     2101 ccagccggct cgaacgattc cgatgctggt gagaaggata aagatggcca agccgacagt
     2161 cgcgtatcgg aaggcgcacg cgacgcgcag aagccgcgcg tacacggcga tgattcgcct
     2221 gaagccacgg tcgacgaggt ctcgcagtcg agagtgcggt tgttctggtt tgggggcggt
     2281 gagcgagtga gccagatgac acggcagact ggtcaaagcc tcgaccaggg acatgagcag
     2341 gcaggatatg acggtgatgg gaaggaccgc gatgaacttg cccatgatgc cggtgatggc
     2401 gagcagtggc agaaaggcca cgatggtcgt agtcaccgag gccagtaccg gccagaaaac
     2461 gcgggcggtt ccatacaatg cggctctggc tggattctga aaagggttct tctcgagctg
     2521 gctataggtg ctttctgaga tgacgatcgc atcatcgacg atcatgccga gcgccatgat
     2581 caggccgaac atgctcatca tgttcaatgt ctgatccatg atgcccagca acaggaatga
     2641 acccagcatc gacacaggaa taccgaacga cacccagagc gagagcttga gatccatgaa
     2701 cagccagagg cagacaaaca ccaggatgag cccctgcaga ccgttgctgc tgagaaggtc
     2761 gaggcgactg cgcaccgcga ccgacgtatc attgagaatc gtcagtttca cggccggcgg
     2821 cagttcgagc cgttttcgct ccgcatactc tcggacggta tccgcaatga cgagagcgtc
     2881 ctcctcttcg gtcttgaaca cagagaccat acaagccttt tccccgttca gcgaggcttc
     2941 gatctggccg tcggcaaagc cgtcgcgcac gtgcgcgatc tgggacaggc gaatggtcgc
     3001 gccgctcgag gcggtccgaa tgaccaggtc ctcgaactcg cgtctgatgt atttctgtcc
     3061 ctttacccgc agcaggactt cgccgccggg agtgcgtaca accccgcccg acagatcgag
     3121 gctgttggtg cgaatcgcac gggcaacatc atcgaagctg agtccgaatt cgcgcagggt
     3181 ggcttccgag acctcgatcg acacttcgtg atctttcaga ccgtcgagca tgatctgtga
     3241 aatctcgggc agggcaagca ggtcccgccg aatctcttcg gcgagctcgc gcatggcttc
     3301 gtcggtcagc gcgccggaga ccacgagctg tagcgtctgt ctgatgagtt cgagcttctt
     3361 gatgacgggc ttctcggcat ctcgcgggaa cgtattgatg ctgttgatgc gagtttcaat
     3421 ctcgttcttg agttcatcga tgtcctcgcc ggtttcgatt tccgcgatca cgattccggt
     3481 actttcacga gcggtggctg acactttctt gatgccggcg agaccggtca gggcctcttc
     3541 gatcttgata cagacgcctt cttcgacctc ttccggactg gctcccggaa agacgaccgt
     3601 gatcagcaca cgatctagcg agaacgaagg aaagatctcg cgtttcatcg agtagaggga
     3661 gatccctccg gagatgatga gaaaaagaac gagtagattc accgaaaccg ggttgttgat
     3721 gcaccagcga atgtatctca tgacgtgagt ctcaggggac ctcagcgctg gaagcgatac
     3781 tcaggcgcat tccctcgacg gcccgggcaa gaggagtcac tacgagctgc tcgtcagagg
     3841 caagaggcgc tcgaatgaac gcttcatcgc catcgagtcg cagcgtttcg acctgtcgca
     3901 gcagcagcct tccgtcttta tagagcggaa tggtgtcttc acgagagacc agcgcgcgcg
     3961 ggactttgta gacctgctgc agcggacgcc ccagcagcga gacttcgacg aacatgcccc
     4021 gcagcagcgg cagaaacagc gaactgctgg cggaaaagtt cgggctgacg gcaacgacca
     4081 gctcgagggt gcgggttttg tcagccagcg agggcgcgac gcgggtgatt gcgccggtcg
     4141 atcgcgtctg ggacgatttc t
//
LOCUS       DAORHD010000283         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23214_length_4147_cov_3.698680, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000283 DAORHD010000000
VERSION     DAORHD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23214_length_4147_cov_3.698680"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PKO06_07275"
     CDS             complement(<1..1097)
                     /locus_tag="PKO06_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV69478.1"
                     /translation="MTTNTGNPPPGSSSPASGNSGRHPPETPIGAHVSRNPAPVETDV
                     MLEELIKAVRSYNQDSDEDRIRKAYAFAKSCHKDQFRASGESYFIHPHAVSLIVAREL
                     RLDSTSICASLLHDVVEDTAATIQDIETQFGPTMARLVEGVTKLNKMFFSSPTAAQAV
                     NFRKMLFAMTNDVRVILIKLADRLHNLRTLQFLPQVKQKYVAKETLDVYAPLAHRLGI
                     HKVKSELEDLSFKYLYPEDYQKIIDFLTSKGLEDDAKLSEAMAEVQKHLKEVNINAEI
                     SGRPKHIFSIWGKMQKHGLPIEGLYDFLGIRVLTRSVRDCYGALGIVHKFWKPIPGRF
                     KDYIAVPKPNMYQSLHTTVIYEGKPLEVQIRT"
     gene            complement(1094..1483)
                     /locus_tag="PKO06_07280"
     CDS             complement(1094..1483)
                     /locus_tag="PKO06_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV69479.1"
                     /translation="MTPESMKGEQAVAMTLTIECFEREESVVVIDVKGEITFENADLL
                     RDKLNELCDQGKCRLIIDLANLKYMSSAGMGVLVHGLKRTRQGKGDLKLVNLNAKMRR
                     VFLITQFTHHFSVFPTLDEAVQSFKTP"
     gene            complement(1585..2247)
                     /locus_tag="PKO06_07285"
     CDS             complement(1585..2247)
                     /locus_tag="PKO06_07285"
                     /inference="COORDINATES: protein motif:HMM:NF003992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNV69480.1"
                     /translation="MADRVTPPQATIRRLSIYLRCLNSLEQRGIKVISSQDLAGHLDL
                     NPAQVRKDLAFFGEFGKRGVGYPVPKLRDHLNKILGLQKRRHVAIVGAGGRLGHALTL
                     YKGFERRNFKVVALFDKDPNVIGQVVGDAGPIYHISEIPRLVRQLAIEMVVLTVPAEA
                     VQELYDYVVLSGVKAVLNFAPVNLNSTGEICVHNVDLAMELEYLSYHLTRHELGLPLK
                     AF"
     gene            complement(2501..3115)
                     /locus_tag="PKO06_07290"
     CDS             complement(2501..3115)
                     /locus_tag="PKO06_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69481.1"
                     /translation="MKKLNLRVLGNVLKGLLNGSIELIAVPTGKKAVRMPVMRAAKPK
                     KPGRKPLSPSAKKIRQMRAKARLVRQAKKSLPAPRDVFLALEGKTDGLKLSQLSNQFK
                     VKRNLLRDMLDRLVAKEDLATLKGRYFLARRLRHKNGDRKERQPPISEKQILDYLKKH
                     QKASLTHMTSALKEKSYQRLIRVINSLRKSKKVVKDGKEYRLAD"
     gene            3318..>4147
                     /locus_tag="PKO06_07295"
     CDS             3318..>4147
                     /locus_tag="PKO06_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69482.1"
                     /translation="MIFRVGILFTILCLTIFSTPAISQDLDSVIDSSPTTDDTASTTI
                     TATGPGINWDEIIFDEPLTAAQLFASQTTDLQLQGFSEGLSPFGILIQIIRQLTQNFG
                     APPVSPIPGPASSTPSVTPIPPTGTGTAVASSASPVTPGPGAQGGSEDDYAQRAQTII
                     DELKAQGAKPGMGIGDSPAKLQIGGFPSWFGELQSLIVGRRSWGELEERGRRLLGEYA
                     ATWSRANIPQPTPHGLTEFFKYLGCCETNDEAIRNYDASLPRSGDFFGSKGGVNWCAF
                     A"
BASE COUNT          898 a         1195 c         1060 g          994 t
ORIGIN      
        1 gtgcgaatct gcacttcgag cggcttgcct tcgtagatca cggtcgtgtg caacgactgg
       61 tacatgttgg gcttgggcac agcaatatag tctttgaacc gtcccggaat cggcttccag
      121 aacttgtgca cgatgccaag tgctccgtaa cagtcgcgca cagagcgggt cagcacccga
      181 atgccgagaa aatcgtacaa gccctcaatc ggcaaaccat gcttctgcat tttgccccag
      241 atagaaaaga tgtgttttgg gcgcccggaa atctcggcgt tgatgttcac ttctttcagg
      301 tgcttctgaa cttcggccat cgcctcagaa agcttggcgt catcttcaag ccccttgctg
      361 gttagaaaat cgatgatctt ctggtaatct tcgggataca ggtatttgaa ggacaggtct
      421 tccagttccg acttgacttt gtgaatcccg aggcgatgcg cgagtggcgc gtagacatcg
      481 agcgtttcct tcgccacgta cttctgcttc acctggggca ggaactgtaa agtgcgcaga
      541 ttgtgcaagc gatcggcgag cttgatcagg atcacgcgca catcattggt catcgcaaac
      601 agcattttgc ggaaattgac cgcctgcgcc gccgtcggcg aactgaagaa catcttgttg
      661 agcttggtca cgccttcgac aagtcgggcc atggtcggac cgaactgcgt ttcgatgtcc
      721 tgaatagtgg ccgcggtatc ttccaccacg tcgtgcaaaa gcgaggcaca gatcgaagta
      781 ctgtcgagtc gcagttcccg ggcgacaatc aacgaaaccg catgcggatg aatgaaatac
      841 gattcaccgg acgcgcgaaa ctgatcttta tggcaggatt tggcaaacgc atacgctttt
      901 cggatgcggt cttcgtccga atcctggttg tacgatcgca cagccttgat cagttcttca
      961 agcatcacgt cggtctcgac cggcgcggga tttcgcgaga cgtgagcccc gattggcgtc
     1021 tcgggcggat gtcggccgga gtttcccgag gcaggggaac tgctaccggg gggcggattc
     1081 cccgtgttgg tggtcatgga gtcttgaaac tctgaacggc ctcatcgagc gtcggaaaca
     1141 ccgaaaaatg gtgcgtgaac tgcgtgatga gaaacacccg gcgcatcttg gcattcagat
     1201 tgaccagctt cagatcgccc tttccctgtc gggtgcgctt gaggccgtgt accagcaccc
     1261 ccatcccggc agagctcata tacttcagat tcgcgaggtc gataatcagg cgacatttgc
     1321 cctgatcaca caactcgttc agtttgtccc gcagcagatc tgcgttttca aaggtgatct
     1381 cgcccttcac gtcgatgacc acgacgcttt cttcacgttc gaaacattca atggtcaaag
     1441 tcatggcgac tgcctgttcc cctttcatcg attcaggtgt cacggggaag ctcctccagt
     1501 attgggggat acacacaccg atcaacaata acatcgtgct cgagccatgt caacgaaccc
     1561 agacacgata cgacttgtac gaaatcagaa ggccttcagc ggcaaaccca gttcatgcct
     1621 ggtcagatgg taggagagat actccaattc catggccaga tcgacgttgt gaacgcagat
     1681 ttccccggtt gaattcaggt tgaccggcgc aaaattgagc acagccttga ctcctgaaag
     1741 cacaacgtag tcatagagtt cctgtaccgc ctcggccggc accgtcaaca ccaccatttc
     1801 aatcgcaagt tggcggacca gacgcggaat ctcggaaata tggtagatcg gcccggcatc
     1861 gcccaccacc tgtccgatga cgttcggatc tttgtcaaac agagccacaa ccttgaagtt
     1921 tcgccgttca aaccctttat agagcgtcag cgcgtggccg agacgaccac ccgctccgac
     1981 aatggcaaca tgccgtcgtt tctgcagccc cagaatcttg ttcaggtggt cgcgaagctt
     2041 cggaaccgga tacccgaccc cacgtttgcc aaattcgccg aagaacgcga gatctttgcg
     2101 cacctgcgcg gggttgagat caagatggcc agcgaggtcc tgcgatgaaa tgactttaat
     2161 gccccgctgt tcaagcgaat tcaagcaacg cagatagatc gacagccggc gaatcgttgc
     2221 ctgcggcggg gtaacgcgat cagccaatcg ttctcctgcc accattttct cctgttcgcg
     2281 tgctctgaaa aaccgtagcg gcgagccgca cagtcgtttc ggcaccgcct gctcgacctt
     2341 cccagacttg taccgcttcc cagcaatgga agcagcgatt cgacacttcg tgatttttag
     2401 cacaaccact gccgaagcgc aatcttcttc tctttgcacc gcaaagcaaa acgccgcgtt
     2461 ccttctgaaa cgcggcgttt tgctttggtg cagccaacga tcaatcggcg agccggtact
     2521 ccttgccgtc tttcacgact ttcttgctct tgcgcagcga attgatgact cggatcaatc
     2581 gctgataact cttctctttg agcgcagatg tcatgtgtgt caaagacgcc ttctgatgtt
     2641 tcttcaaata gtcgagaatc tgcttttcag agatcggtgg ctggcgctct ttgcgatctc
     2701 catttttgtg gcgcagacgt cgagccagga aatatcgccc cttcaacgtc gccagatctt
     2761 ctttggccac caggcgatca agcatgtcac gcagcaggtt tcgtttgacc ttgaactgat
     2821 ttgacagctg cgagagcttc agtccgtcgg tttttccttc gagggccaga aaaacgtcgc
     2881 gtggggctgg cagagatttt ttggcttgcc gtaccaaacg agccttggcc cgcatctgcc
     2941 gaatcttttt ggcagaaggc gacagaggtt tacgacccgg tttcttcggt ttggcggctc
     3001 gcatcaccgg catgcgcacg gctttcttac cggtaggaac cgcgattaat tcgatgctcc
     3061 cattcagcaa tcctttcagg acattcccga ggacacgaag attcagcttc ttcatgaaaa
     3121 ctcctgttgc ttaatatgct ctcgatggca gattacacaa atgattccct gcaggcaagt
     3181 actatttttt tctaatctta tactactatt cagtgttcat cagcattttc tcttcatgtt
     3241 acgtggaact tttttccgtg atgtcgatcg tgttacactg cgcctgtttc cctagaagta
     3301 ccgcaggagg catgaacatg attttccgcg tcgggattct cttcaccatt ctttgtctca
     3361 ccattttttc gaccccagcc atcagccagg atctcgattc cgtcatcgat tccagcccga
     3421 caaccgatga cacggcctcc acgacgatca cggcaaccgg acctggaatc aattgggatg
     3481 agatcatttt cgacgaaccg ctgacggccg cgcaactctt tgcttcgcaa acgaccgatc
     3541 tgcagctgca gggcttcagt gaaggactct cccccttcgg catcctcatt cagatcattc
     3601 ggcagctcac ccagaatttt ggcgcccccc cagtatctcc gattcccggc cctgcaagca
     3661 gcaccccgtc cgttactccc attccaccga caggtaccgg aaccgccgtc gcctcttcgg
     3721 cctcaccggt tactcccggc cccggggctc agggcggcag tgaagacgat tacgcccaac
     3781 gggctcaaac aatcattgac gaattgaaag cccagggtgc aaagcccggt atgggaatcg
     3841 gcgattctcc ggccaagctg cagatcggcg gttttccgtc ctggtttggc gaactgcagt
     3901 cgctgatcgt tggccgacgc tcctggggcg aactcgaaga gcgtggccga agactgctcg
     3961 gtgaatatgc cgccacctgg tcccgcgcca acattccaca accgactccg catggtctga
     4021 ccgagttttt caagtatctc ggctgctgcg aaaccaatga tgaagctatt cggaactatg
     4081 atgcctcgct gcctcgcagc ggtgatttct tcggttccaa gggcggggtg aactggtgtg
     4141 cgttcgc
//
LOCUS       DAORHD010000284         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23224_length_4146_cov_2.788560, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000284 DAORHD010000000
VERSION     DAORHD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23224_length_4146_cov_2.788560"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKO06_07300"
     CDS             complement(<1..687)
                     /locus_tag="PKO06_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNV69483.1"
                     /translation="MEILERARFSLLYDLQKAGYDLQQKLAIRPLNPDEAIGPTASPE
                     LAIKRGPEKVIQAEVNGVIGQAFTEQPASWSGTVHQALQLHLTSVQNRAFVVAAINAL
                     MKATNRCTGSAHCRDGDPEACGTCLVKEIEQRFPQPIVGMFGFQPFLVKALVDRFGSK
                     SVLVADLNPNNIGQIKAGVRIMDGEKDAERLAADCTFGLCTGSSIVNGTFDELWQLFS
                     RHRKPVLFFGN"
     gene            complement(787..1200)
                     /locus_tag="PKO06_07305"
     CDS             complement(787..1200)
                     /locus_tag="PKO06_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV69484.1"
                     /translation="MIIAIPLAGGVLSEHFGHCEEFAFIEADEQKKTIIKETRQQPPE
                     HEPGVLPRWLLSNKANVAIVGGMGMRARQMLEAQGVKVIMGAPAKAPVDLAREYLAGT
                     LVGGDNTCGHGPDHVCDGNHDHGHQHKHPHIVVKK"
     gene            complement(1226..2464)
                     /locus_tag="PKO06_07310"
     CDS             complement(1226..2464)
                     /locus_tag="PKO06_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HNV69485.1"
                     /translation="MDSSHKTQFDTACIHAGQHPDELFGGVSVPIYQSSTFAFANAEQ
                     GAARFSGQSDGYKYTRLGNPTTAALEACIAELEGGGVSMATASGMAAVSTAYMALLGH
                     GAHAVSTDSVYGPSRTLLEKEFTRYGIQVDFVDTSNLEQLKKVLRPNTRLLYLETPAN
                     PTLSITDIASASRLAHEVGAVVVVDNTFCSPMLQRPLDLGADIVLHSMTKFLNGHSDV
                     VAGILVTRSPELAARLKTVLVQMGGTIDPHQAWLVLRGVKTLALRIRAAQENAIRLAT
                     DLQQHPLVSSVRYPGLKDHPQHELSKMQMKGPGSMISFELNGGVEAGRLLMNTVKLST
                     LAVSLGGIETLIQHPASMTHASVSRESRIAAGISDGLVRLSVGCEAYEDLRADLLSAL
                     DSVRQQLTGADLHAKLNVNV"
     gene            complement(2471..3382)
                     /locus_tag="PKO06_07315"
     CDS             complement(2471..3382)
                     /locus_tag="PKO06_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69486.1"
                     /translation="MAITEFVVISGKGGTGKTTVTASLAAHIGRAVLTDADVDGANLE
                     LALQADLAREESIIGGKKAQIKPSLCIACGKCADLCRFSAVIPGAPHSSGGVIMAIDH
                     DLCEGCGLCIRFCPSNAIGFRESKGGTWRHSHTRLGDLFHARLAPGGENSGKLVALLR
                     RKAREAAQTQGVEFMLTDGPPGAGCPVIATLTGAHRVLVVTEPTPSGKSDAERVINLC
                     RHFRRTVSLAINKFDLHAGMAADIEQWAASLHVPVVGKLPYDSIVPKAVRQGVPVVTL
                     PTSAWGQALVEMAQRLELPSINNQHQG"
     gene            complement(3373..>4146)
                     /locus_tag="PKO06_07320"
     CDS             complement(3373..>4146)
                     /locus_tag="PKO06_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV69487.1"
                     /translation="AGYSVQLIDADVEGPNSHHFLPLGAPLDEIVVRSMLPEFDQSLC
                     TLCGQCTEFCAFSALAMTTDRVLLFADMCHGCEGCREICPTQAIRSGSQEIGIILNWR
                     LDGIDFWQGRLHTGQVLTPALIRELKRRAGERTADLTIVDCPPGTACAMMAAVKNADH
                     LLLVTEPTPFGRHDLELAMAATRRWKTPCEVVVNRSEGEDSLIEELCQARAVPVLDRL
                     PLDRAVATAYAGGHPMYQSGDTWKTLFQNMVRHLEASWR"
BASE COUNT          859 a         1181 c         1205 g          901 t
ORIGIN      
        1 gttgccgaag aacagcacag gctttcgatg acgactgaac aattgccaaa gctcatcaaa
       61 cgtgccattg acgatgctgg aaccggtaca cagaccgaag gtgcagtcag cagcaagacg
      121 ttcggcatcc ttttctccgt ccatgattcg aacgcctgcc ttgatttgtc cgatgttgtt
      181 tgggttgagg tcggctacca aaacagactt ggaaccaaaa cgatcgacaa gagctttgac
      241 gagaaacggt tgaaacccga acatgccgac aatcggctgg ggaaaccttt gctcgatctc
      301 cttcaccagg catgttccgc atgcttcagg atcgccatca cgacagtggg cactgcccgt
      361 gcagcggtta gtggctttca tcagggcatt aatcgcggca accacaaagg cccgattctg
      421 caccgacgtc agatgcagtt gcagagcctg gtgtactgtt cccgaccaac tggccggttg
      481 ttcagtgaag gcttgaccga tgaccccatt tacttctgcc tgaatgactt tttccggtcc
      541 ccgcttgatt gccagttcgg gggatgcggt cggcccgatt gcttcatctg ggttcaatgg
      601 cctgattgcc agtttctgtt gcaggtcata cccggctttc tgcagatcat agagtagtga
      661 aaatcgtgcc cgctcgagta tttccatgag cgttctcctt cttcgattac ggaactctgc
      721 tgcgaacgat ctactttccg gccagaatct tcttggcttc gtccatcatg ttcgagtaca
      781 aacgtcctac tttttgacga cgatatgagg gtgtttgtgc tgatgaccgt ggtcatggtt
      841 cccgtcacag acatgatcag ggccatgacc gcaggtattg tcgccaccga ccagggtgcc
      901 ggccagatac tcgcgtgcca gatcgacggg cgctttggcc ggcgcgccca tgatcacctt
      961 cacgccctgt gcctcaagca tctgacgcgc gcgcatgccc atgccgccga cgatcgcgac
     1021 gttggccttg ttcgacagca gccagcgggg cagaaccccg ggttcgtgct cgggcggctg
     1081 ctgcctggtt tccttgatga tggtcttctt ctgctcgtcc gcttcgatga aggcgaactc
     1141 ttcgcagtgt ccgaaatgtt ccgaaagaac gccgccggcc aggggaattg cgataatcat
     1201 agctggtctc cttttttgtc ttggttcaaa cgtttacatt cagtttcgcg tgcaggtcag
     1261 caccggtgag ttgctgacga acggagtcga gggcagacaa cagatctgcg cggaggtcct
     1321 catatgcttc acaacctacc gataagcgta ccagtccgtc agagatgcca gctgcgatac
     1381 gagactctct tgatacggaa gcatgggtca tgctcgcagg gtgctgaatc agtgtctcga
     1441 taccacctag ggaaaccgcg agtgtcgaga gctttacggt gttcatcaga agtctccccg
     1501 cttcaactcc tccgttcagc tcaaatgaga tcattgatcc cggacctttc atctgcatct
     1561 tggacaactc atgctgggga tggtccttta aaccgggata ccgtacggat gacaccagcg
     1621 gatgctgttg caaatcggtc gccagccgaa tggcgttttc ctgcgcggca cgaatgcgca
     1681 aggctagggt tttgaccccg cgtagaacca accacgcctg gtgcgggtcg atagttccac
     1741 ccatctgcac gagaacggtt ttcagacgag cggcgagttc aggactgcga gtgacgagaa
     1801 tcccggcaac gacgtcgctg tgaccattca gaaacttggt catactgtgc agtacgatgt
     1861 cagcaccaag atcgagtggg cgctgcagca tgggactgca gaaggtgttg tcgaccacca
     1921 cgaccgctcc gacttcgtga gccaggcgcg aagcgctcgc gatatcggtg atcgagagcg
     1981 ttggattggc aggagtttcg agatacagca gacgggtgtt cgggcgaaga acctttttga
     2041 gttgttccag gttcgaggtg tcgacgaagt cgacctggat cccgtagcgg gtaaattcct
     2101 tctcgaggag ggttcgcgat ggaccataca cagagtccgt ggaaaccgcg tgtgctccgt
     2161 gacccagaag tgccatatag gccgtgctga ccgcggccat gcccgaggcc gtagccatgc
     2221 tgacaccgcc gccctcaagc tcggcgatgc acgcttcgag tgccgcggtg gtcgggtttc
     2281 cgagccgagt gtatttgtac ccgtcactct gtcccgaaaa tcttgctgct ccctgttcgg
     2341 cattggcaaa agcaaaagtc gatgactgat agatgggtac gctgaccccg ccgaatagtt
     2401 cgtcagggtg ctggcctgcg tgaatacagg cagtgtcgaa ttgggttttg tgtgacgagt
     2461 ccatgtaatc ttatccttga tgttggttgt tgatcgaggg aagttcgaga cgctgggcca
     2521 tctcgacgag ggcctgaccc caggccgaag tcggaagagt cacgacggga acgccctgcc
     2581 gaaccgcctt gggaacaatc gagtcatacg gcagtttgcc cacgaccggc acgtgaaggg
     2641 aggccgccca ctgctcaatg tcggcagcca ttccggcatg cagatcgaat ttattgatcg
     2701 ccagcgagac cgtgcggcga aagtgtcgac acaggttgat cactcgctct gcgtcagact
     2761 ttccggaagg cgtgggctcg gtgacgacga gaactcggtg tgcgcccgtc agggtcgcaa
     2821 tgaccgggca gccggctccc ggagggccat ccgtcagcat gaactcaacg ccctgagtct
     2881 gggctgcttc gcgtgctttg cgcctgagca aagccaccag tttgcctgag ttctcgccac
     2941 cgggggcgag acgagcatgg aacagatcgc ccaagcgcgt gtgactgtgg cgccaggtgc
     3001 cacccttcga ctcgcgaaac ccgatggcgt tcgatggaca gaaacgaatg cagagaccac
     3061 acccttcgca gaggtcatgg tcgatggcca tgatgactcc gcccgacgag tgcggcgcac
     3121 caggaatcac ggcactgaac cggcaaagat cggcacactt gccgcaggca atgcagagag
     3181 agggcttgat ctgtgccttc ttgccgccga tgatgctttc ctctcgcgcc agatcagcct
     3241 gtaatgccag ttcgaggttg gcgccatcaa cgtcggcatc ggtgagcact gcccgaccga
     3301 tgtgtgctgc cagagaagcg gtgaccgttg ttttccccgt gccacctttc ccactgatca
     3361 ccacaaattc tgttatcgcc atgacgcctc caggtgtcga accatgtttt ggaacagggt
     3421 cttccaggtg tctccggatt gatacatggg gtggcctccg gcgtaggccg ttgccactgc
     3481 gcggtcaagc ggcagacgat cgagcactgg aaccgcacga gcttggcaca gttcttcgat
     3541 cagactgtct tcgccttccg agcgattgac gacgacctcg cacggggttt tccagcgccg
     3601 agttgcggcc atggcgagtt ccaggtcgtg tctgccgaag ggcgtcggtt cggtcaccag
     3661 aagcagatga tccgcatttt tcaccgcggc catcatcgcg caagcagttc ccggaggaca
     3721 gtcgacgatc gtgagatcgg cagttcgttc accggctcgt cgcttcagtt cacgaatcaa
     3781 ggctggggtc agaacctgcc ccgtgtgcag acggccctgc cagaaatcga tgccatccag
     3841 gcgccaattc agaataattc ctatttcctg agacccggaa cggatggcct gtgtggggca
     3901 gatctcccga cagccctcac acccatgaca catatcggcg aacaacagga cccggtctgt
     3961 cgtcatggcc agggcggaaa acgcgcagaa ctcagtacac tggccgcaca gcgtgcagag
     4021 agattgatca aactctggca gcatggagcg gaccacgatc tcatcgagtg gtgcgccgag
     4081 cggcagaaag tggtggctgt tcggcccttc gacgtcggcg tcaatcagtt gtacggaata
     4141 ccctgc
//
LOCUS       DAORHD010000285         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23238_length_4145_cov_3.109291, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000285 DAORHD010000000
VERSION     DAORHD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23238_length_4145_cov_3.109291"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKO06_07325"
     CDS             complement(<1..844)
                     /locus_tag="PKO06_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV69488.1"
                     /translation="MNRWYKDAVFYETHVRCFFDGNNDGIGDFLGLRQKLPYLRDLGV
                     TCLWLLPFYPSPLKDDGYDIANFRDVHPNYGTLDDFRCFLDEAHDMGLRVLADLVVNH
                     TSDQHHWFQEARRSPQSPFRDWYVWSDTDDRYQGTRIIFKDTEVSNWTWDPMAKQYFW
                     HRFFHHQPDLNYDNPDVQQEMLDIVAYWLSLGLDGFRVDAVPYLFEREGTCCENLQET
                     HQFCKRLRAMVDEKFPDRVLLAEANQWPVDLLPYFGSGDEFHMAFNFPLMPRLFIALR
                     QECKE"
     gene            complement(866..3070)
                     /locus_tag="PKO06_07330"
     CDS             complement(866..3070)
                     /locus_tag="PKO06_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HNV69489.1"
                     /translation="MSRLLIVSNRLPVTVKAEHGQIQVTESAGGLATALRGPHARMES
                     LWIGWPGDTSRLTNVQESEHEKRLGELRTKAVKLSATEVAHFYDGFSNGVIWPLYHYL
                     TDKVDRAAWMNWKSFVEVNQKFAEAVVCEYRQGDVIWVHDYQLSLVPAMIRRRLPEAV
                     IGFFLHIPFPSSEVFRILPWRNEILEGMLGADLIGFHTYGYLHHCSRALLHLLDLEVT
                     EGIVRLADRQVLLGVFPIGIDTALFEGLSSSAEVVAEVEIIRREAGGRKLLLGVDRLD
                     YTKGLVRRLMAIERLLEREPSLREQIRFVQLIVPSREKVPSYARLRRELDETVGRINA
                     IYGSVSSLPVHCLYRGVSTQKLTALYRTADVMLVTPLRDGMNLVAKEYVACRSDEDGV
                     LVLSEFAGAASELVESLLINPYDIDRLALTIKRALDMPRGEQCSRMRRLRERVRRFDA
                     FRWSQLFLETLESAGVLRMKQPRAAWMADDERERLIAKIQGYPSLHLILDYDGTLVPF
                     AASPELAAPDQRLLDLLHRLALRPRTQLHIVSGRPREILEAWFAGLPIGLHAEHGGWS
                     WLPVKQTWISVAEELPSWKSEVQKTLERITTETPGSFIEEKSLGLAWHFRQVPSEFGR
                     AHAASLVQRLRQELKDLPIEILNGEKVVEVRARGINKGIIVQRLQEAASGAAEFVAIG
                     DDRTDEDLFHAVAGHGVCIHVGAGPSQARYRLEAPWAVRAFLERILHDGAEK"
     gene            3334..3783
                     /locus_tag="PKO06_07335"
     CDS             3334..3783
                     /locus_tag="PKO06_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69490.1"
                     /translation="MSSNYSVLCVRQEPSEPFQSHQEIFDSPPFENQDFSELSQRHGE
                     IIYLAEQENVGFVHYEHWSNGSLLRRLKYNNDGSWLSADGEPEAWESPILFSPALLEK
                     TLQNWDADGHTQIREAWSRQRIQEGDLFPILGLLDFIGDLRRFWKLP"
     gene            3878..>4145
                     /locus_tag="PKO06_07340"
     CDS             3878..>4145
                     /locus_tag="PKO06_07340"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HNV69491.1"
                     /translation="MKKITAIEYRSDKAKSVSVAGDFNDWCGSAKGRFDPAIGEMKPE
                     GFGRWRFSVHGIAPGRHEFKFVIDGTWEPGPNRVLHLDERGHLFD"
BASE COUNT          904 a         1296 c         1125 g          820 t
ORIGIN      
        1 gctccttgca ctcctggcgt agtgcgatga agaggcgcgg catcaacggg aagttgaaag
       61 ccatgtgaaa ctcatcgccg ctgccgaaat acggcagcag gtcaaccggc cattgattgg
      121 cttccgccag cagcacccgg tccggaaact tctcatcgac catcgctcgc agtcgtttgc
      181 agaactggtg cgtttcctga agattctcgc agcaggtgcc ctcgcgctca aacagatacg
      241 gcaccgcatc gacccgaaaa ccgtcaagac ccagcgaaag ccagtaggca acgatatcca
      301 gcatctcctg ctgtacgtcc ggattgtcgt agttcaagtc gggctggtgg tggaagaacc
      361 ggtgccagaa gtactgtttc gccatcgggt cccaggtcca gttcgagact tccgtgtcct
      421 tgaagatgat gcgcgttccc tgataccggt cgtcggtgtc actccagacg taccagtcgc
      481 ggaagggcga ttgcggcgat cggcgagcct cctggaacca gtgatgttga tccgaggtgt
      541 gattcaccac cagatcggcc aatacgcgca agcccatgtc gtgtgcctcg tcgagaaagc
      601 agcgaaaatc atcgagcgtg ccatagttcg gatgcacgtc acgaaagttg gcgatatcgt
      661 acccgtcatc cttcaagggg gacgggtaga acggcagcaa ccagaggcag gtcactccga
      721 gatcgcgcag atagggcagc ttctgtcgca gaccaagaaa gtcaccgatg ccgtcgttgt
      781 tcccgtcaaa gaagcagcgc acgtgcgttt catagaagac cgcatccttg taccagcggt
      841 tcatgggcgt ttctcccttt taatcttact tttccgcgcc atcatgcaga atgcgctcga
      901 ggaatgcccg aacagcccag ggggcttcga gtcgataacg cgcctgcgag ggaccagctc
      961 ccacatggat acagacacca tgaccagcca ccgcgtgaaa caggtcctcg tcggtacgat
     1021 cgtctccgat cgccacgaac tccgcggctc cgctcgccgc ttcctgtagc cgttgcacga
     1081 tgatgccctt gttgatgccg cgggcacgca cctccaccac cttctctccg ttcaggatct
     1141 ctatcgggag atccttcaat tcctgccgca atctctgcac cagcgaggcg gcatgcgccc
     1201 gaccgaattc gctggggacc tgtcgaaaat gccaggcgag acccaggctc ttttcttcga
     1261 tgaatgatcc tggagtctcg gttgtgatgc gttcaagcgt tttttgcacc tcggacttcc
     1321 aggatggcag ttcttcggca acggagatcc aggtttgttt caccggcagc caggaccagc
     1381 caccgtgctc cgcatgtaaa ccaatgggca gacccgcaaa ccaggcttcc agaatctctc
     1441 gcgggcgtcc gctgacgata tgcaactgcg tccgtggccg cagcgccagt cggtgcagca
     1501 gatccagaag tcgctgatcc ggagccgcca gttccggcga agcggcaaac ggcaccaacg
     1561 taccgtcgta gtcaaggatc agatgcagac tgggatagcc ctgtatcttg gcgatcaacc
     1621 gctcccgttc gtcgtctgcc atccaggcgg cacgcggctg cttcatccgt aacacgccgg
     1681 ccgactcgag ggtttcgaga aacagctgcg accagcgaaa ggcatcgaat cgccgcacgc
     1741 gttcgcgcag tcgccgcatg cgcgagcatt gttccccgcg cggcatgtcg agcgctcgtt
     1801 tgatcgtcag cgccagtcga tcaatgtcat acggattgat gagcaatgat tcgaccagct
     1861 cggacgcggc tccagcaaac tcgctgagca cgagcacgcc atcctcgtcc gaccggcagg
     1921 ccacatactc cttcgcgacc agattcatgc cgtctcgcaa cggcgtcacc agcatgacgt
     1981 cggcggtgcg gtacagggcg gtcaacttct gcgtcgacac gccgcgatac agacagtgca
     2041 ccggcagcga agacaccgag ccataaatcg cattgatgcg accgaccgtt tcatcaagct
     2101 ctcgacgcag gcgcgcatac gacggcacct tttcgcgcga tggcacgatc agttgcacga
     2161 agcgtatctg ctcacggagc gacggctctc gttcgagcag acgctcgatc gccatcagac
     2221 gacgcaccaa ccccttcgta tagtcgaggc ggtcgacacc gagaaggagt ttgcgaccgc
     2281 cagcctcgcg ccggatgatc tccacttccg cgaccacctc ggctgaactt gaaagtccct
     2341 caaagagcgc cgtgtcgatc ccaatcggaa agaccccgag cagcacctgc cggtcggcca
     2401 gtcgcacgat gccttcggtg acctccagat ccaggagatg cagcagagcg cgggaacagt
     2461 gatgcagata cccgtaggta tgaaagccaa tcagatcggc accgagcatg ccctcgagaa
     2521 tctcgttgcg ccagggcaga atacgaaata cctccgaaga gggaaagggg atgtgcagaa
     2581 agaagccgat gacggcctcc ggcagccgac gccggatcat cgccggcacc agtgacaact
     2641 gatagtcatg cacccagatc acgtcgccct gacggtattc gcagaccacg gcttccgcga
     2701 acttctgatt cacctcaacg aagctcttcc agttcatcca ggccgcgcga tcgaccttgt
     2761 cggtcagata gtggtacagc ggccagatga cgccgttgga aaacccgtcg taaaagtgag
     2821 ccacctccgt cgcgcttaat ttcacagcct ttgttcgaag ctcaccgaga cgtttttcat
     2881 gctctgattc ttgtacattc gtcagtcgcg acgtatcacc cggccacccg atccataacg
     2941 attccatgcg tgcatgcggc ccgcgcagcg ccgttgccag tcctccggca gactcagtca
     3001 cctgaatctg tccatgttcg gctttcacgg tcaccggcaa gcgattcgaa acgatcagca
     3061 gtcgtgacat gacaccctcc tgcaactgtg taatcttgcg gatggcggcc cgaccatcac
     3121 tctcaccttc gggctctctc tcttgcccga acccgttccc atgttaccgg ttttctgctg
     3181 tctctttcaa ccctggcaga ggtcatcgct tcccttcatg ggtgggaatc ccgtatcatg
     3241 agagcatcgc gaacgccgga gagcattcgt cggttcacga gtgtgcgcaa cccacggtca
     3301 cggctgcggt caaacagtga agcaggagaa cacatgtctt cgaactactc cgtcctctgc
     3361 gtccgccaag agccatcgga accattccaa tcgcatcagg aaatcttcga ttctccaccg
     3421 ttcgagaacc aggacttttc tgagttgtcg cagcggcatg gcgaaatcat ctatctcgcc
     3481 gaacaggaga acgtcggatt cgtccactac gagcactgga gcaacggcag tctgctacgc
     3541 cgtctgaaat acaacaacga cggttcgtgg ctcagcgctg acggagagcc tgaagcctgg
     3601 gaaagcccga ttcttttttc ccccgccctt ctggaaaaaa ccctgcaaaa ctgggatgcc
     3661 gacggacaca cacagattcg cgaagcctgg tcccgtcagc gcattcaaga gggcgacctg
     3721 ttccctattc tgggtttgct cgatttcatc ggcgacctcc gccggttctg gaaactgccc
     3781 tagtgcgaag gcgttcatcg tcgcccatcc gtctcgctcc gaaatgcatc gctgaagctc
     3841 ttggaatcac cgggaaaata cggtaagatg cccgcgcatg aaaaagatca cggccatcga
     3901 gtatcgatcc gacaaagcca agtccgtttc cgtcgccggt gatttcaatg actggtgcgg
     3961 atcggccaaa gggcgcttcg acccagccat cggggaaatg aaaccggaag gattcggtcg
     4021 ctggagattt tccgtgcacg gcatcgcacc cgggcgacat gaattcaagt ttgtgatcga
     4081 cggcacctgg gaacccggcc cgaatcgcgt gttacacctc gacgaacgcg gacatctgtt
     4141 cgacc
//
LOCUS       DAORHD010000286         4138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23296_length_4138_cov_3.494734, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000286 DAORHD010000000
VERSION     DAORHD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23296_length_4138_cov_3.494734"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKO06_07345"
     CDS             complement(<1..256)
                     /locus_tag="PKO06_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69492.1"
                     /translation="MRTNSFIILLLFVCVRVFAAGAAMPAPSPDECLVIGGGSDPAKG
                     ASFFAQAIHAWRLGAASQALQHYEQAIIADRGVLSRDDEGM"
     gene            387..971
                     /locus_tag="PKO06_07350"
     CDS             387..971
                     /locus_tag="PKO06_07350"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV69493.1"
                     /translation="MMSRLSPLSEHEPFLSIYRQLSNVSMNPRLHTCTTVFEHAELVR
                     QTIARLGRQQGSSVAELVLFDDLALAHDIGKLRGHPHEHHSVAILQDLGETDAAFLSL
                     VKHHDIALPWYRSTQQGTPPTDAAWLRLAAQFDLWLLCLFAIADRADCPGGWQQNPPT
                     IWFLQEVKRRNLVRRSLPSFDVPSTNPPPDASSP"
     gene            1226..2182
                     /locus_tag="PKO06_07355"
     CDS             1226..2182
                     /locus_tag="PKO06_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV69494.1"
                     /translation="MKESDISNILATAGIFSGLSPESYLPIIREARQVHVGKGECLLD
                     PLSENDSLFILLSGELLVCLDPAPESSVAQIRPGECAGELSIIDDRPPSAYVIGAVPS
                     ELIALNRRTLSALVKRLPLIAVNLLPILSDRIRQNNACLRKSLEEQRRFQNKAETDAL
                     TKLHNRAWLNEIFPRQLDLGERTGQPVSVGFVDIDHFKKVNDTHGHSVGDLALAHVAT
                     CVCRGLRASDMCARYGGEEFAFMLPATDLTQARVIAEQVRRMVAGTPLDLREGETLPL
                     TISIGIGEQKPGETLDALLKRADAALYTAKKEGRNCVRVATS"
     gene            2435..3367
                     /locus_tag="PKO06_07360"
     CDS             2435..3367
                     /locus_tag="PKO06_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV69495.1"
                     /translation="MRKAFRRQTVVHICLLMVSLAGLLATGILGQDAGEKPKRQLRLG
                     YSGTTISKVDVNDAKVAMKLWTEKFSSEEGSNFAAEVFIFNSVDEMKQAVVRGEIDLV
                     AMTTQEYLDLKNEIPLQPAYTAVVGTTQFDRLVLATHRDSPIKSIADLKGKRLNLENY
                     GSGELSTMWLDCLLLRQKLPPSATFLQEVKTFDKASPAVLPVFFQKVEACIAREYSLS
                     VLTELNPQIGKSLRIIEQSPLYQRGMVCFRQGFNKDWQDEITRSAFRLHESTKGQQIL
                     TLFKIDRIIPFDATALASVQELMTEHRSLMSKKP"
     gene            3439..3630
                     /locus_tag="PKO06_07365"
     CDS             3439..3630
                     /locus_tag="PKO06_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69496.1"
                     /translation="MPTERCPLCMKTREFDPAKEQYLKYQNSERLVCRECSAEVEQQM
                     RPTWHIVGNRIFPKSSLSR"
     gene            complement(3677..>4138)
                     /locus_tag="PKO06_07370"
     CDS             complement(3677..>4138)
                     /locus_tag="PKO06_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69497.1"
                     /translation="GIHLIFGLGDTEREFTQTIDWVYAHHGKVGLFALTPVRGAQTSL
                     QPPDLAAWRRMQIFRYLRSLKMLEYKSCRFENDCLSRVPLAADEYERCLADGEAFRTA
                     GCPDCNRPYYNERPGGILYNFPRQLASDEVRQCLAEAGGLTDACNGERNCP"
BASE COUNT          901 a         1234 c         1122 g          881 t
ORIGIN      
        1 ccatgccttc gtcatcacgc gacagcactc cgcgatcggc gatgatcgcc tgctcgtagt
       61 gctgcagggc ctgactcgcg gcccccagac gccaggcgtg aatcgcctgg gcaaagaagc
      121 tggcgccttt ggccgggtca ctgcctccgc caatcaccag gcactcatcg ggggacggag
      181 ctggcatcgc cgcgcccgcg gcaaacacac ggacacacac gaacagcagc agaatgatga
      241 atgagttggt acgcataagt ctatgatacc actaaacact cattcacaaa atgctccccg
      301 atcgccggcc ccgggcacgc agagcgtcgt tgtcacgcac gtctgtggcc cctcggaatc
      361 cggatgcggt agaatgaacc ccgaacatga tgtctcgatt gtcacccttg tccgaacatg
      421 agccgtttct cagcatctat cgccagctgt cgaacgtctc gatgaatccg cgtctgcaca
      481 cctgcaccac ggtcttcgag catgccgaac tggtgcgtca gaccatcgcg agactcggtc
      541 gtcaacaggg cagcagcgtc gcggaacttg tactgttcga cgacctggcc ctggcgcacg
      601 atatcggcaa actgcgcggc cacccacacg agcatcattc cgtggccatt ctgcaggacc
      661 tcggcgagac ggatgccgca tttctttccc tggtcaaaca tcatgacatc gccctgccct
      721 ggtaccgcag cacccaacag ggaaccccgc cgaccgacgc cgcctggctg cgtttggccg
      781 cgcagttcga tctgtggttg ctctgtttgt tcgctatcgc cgatcgtgcc gattgtccgg
      841 gtggctggca gcagaatccg ccaacgatct ggtttctgca ggaagtgaag cgccgcaatc
      901 tggtcaggcg ctcgttacct tcattcgacg tgccttccac caatcctcct cctgatgcat
      961 cttcaccgta gatcgagtat atccggtatc cggctctcct gctccacgtc ttacgattga
     1021 ctgtaccagt cgatggtctc gtcaatcata ccgctgtggt tgcggggttc gcaccgcagg
     1081 cggaactgaa gaagaaagtt cgcgacgaac caccctggcg gaccgtcaaa cgcgtctgcg
     1141 gcttttgtct ttcgtggcat tggcagaaat ctcgtcacgg ggtagaatgc gaaaacgatt
     1201 cgaaccacaa aggggagaag attccatgaa agaatccgat atttccaaca tcctggcaac
     1261 ggccggtatt ttctccgggc tttctccaga gagctatctg ccgatcattc gagaagcgcg
     1321 gcaggtgcac gtcgggaagg gagagtgcct tctcgacccc ctttcggaaa acgattcctt
     1381 gttcattctt ctctctggcg agcttctggt gtgtctcgac cctgcccccg aatcgtccgt
     1441 tgcgcagatt cgaccgggag aatgtgccgg cgaactttcg atcattgatg atcgcccgcc
     1501 ttctgcgtat gtgattggag ctgttccgtc cgagttgatc gccttgaatc ggcgcaccct
     1561 gagcgctctg gtcaagagac ttccactcat tgcagtgaac ctgctcccga ttctcagtga
     1621 tcgaatccgg cagaataacg cctgtctgag aaaaagtctc gaggaacagc gccggttcca
     1681 gaacaaggcg gaaaccgacg ctctgacaaa gcttcataat cgggcctggc tcaacgagat
     1741 ttttccgcgc caacttgatc tgggcgagcg gacaggtcag cccgtatccg tcgggttcgt
     1801 cgacattgac catttcaaga aggtcaatga tacccatggc cattcggtcg gtgatctcgc
     1861 actcgcacac gtggcgacat gcgtctgtcg tggcctgaga gccagtgata tgtgcgcccg
     1921 ctacggcggt gaggagttcg ctttcatgct tccggctacc gatctgaccc aggcccgggt
     1981 cattgccgaa caggttcgcc gcatggtcgc cggaactccg ctcgacctcc gcgaagggga
     2041 aacactcccg ctcaccatct cgatcggcat tggggagcaa aagccggggg aaaccctcga
     2101 tgcccttctc aaacgagccg acgccgcctt gtacacggca aagaaagaag gtcgtaactg
     2161 cgtccgggta gcaacaagtt gaagcagcca acagaattct ttcgcggggt gagagaaccc
     2221 gacttctgcg cgagatcacc tctcttcggc gaaccgctgc gataagagga acctctcgtc
     2281 catgattcaa ctttcgagag ctggtactcg aaccgacgac ctaccagtcg ctctggcaca
     2341 ggtcaaggta gttggtcgtg cacgtcagcc aggcttcttc gcttcttccg ggcaaatgac
     2401 tgcttgtgaa ccgacttcgt gctagactga acacatgaga aaagcgtttc ggcgccagac
     2461 ggtcgtgcac atctgcctgc tgatggtcag tcttgcgggg cttctggcaa ccgggatact
     2521 cggacaggac gctggcgaga agccgaaacg ccaactgcga ctcgggtatt cgggaaccac
     2581 catttccaaa gtcgatgtca acgacgccaa ggtcgccatg aagctctgga ccgaaaagtt
     2641 cagcagcgag gaaggatcga actttgcggc cgaggttttc atcttcaact cggtcgacga
     2701 aatgaaacag gcggtggtgc gcggcgaaat cgacctggtg gccatgacca cgcaggagta
     2761 cctcgatctg aagaatgaga tcccgctcca gccggcgtat acggctgtgg ttggtacgac
     2821 ccagttcgat cgcctcgttc tggcaactca tcgcgattcg ccgatcaaaa gcattgccga
     2881 cttgaagggc aagcgtctga atcttgaaaa ctacggttct ggtgagcttt cgaccatgtg
     2941 gctcgactgc ctgctgcttc gacagaagct tcccccgtcg gccacgtttc ttcaggaagt
     3001 caaaacgttc gataaagctt ctcctgccgt tttgccggtc ttttttcaga aggtcgaggc
     3061 ctgtattgcg cgtgaataca gtctgagcgt cctgacagaa ctgaacccgc agatcggcaa
     3121 gagtctgcgc atcatcgaac agtcgccgtt gtatcagcgc ggcatggttt gctttcggca
     3181 aggattcaac aaggactggc aggatgagat cacccgttcg gctttccgac tgcatgagtc
     3241 aacgaagggg caacagattc tcaccctctt caagatcgat cgcatcattc ctttcgacgc
     3301 aacggctctc gcgtcagtac aggaactgat gaccgaacac cgttcgctga tgtcgaagaa
     3361 accgtaacca caacttgctg agaggctctg gttgcacctt tccccggctt atggtagctt
     3421 tttcgcaagg aaaccagcat gccaaccgaa cgatgtccac tctgcatgaa gaccagggaa
     3481 ttcgacccgg cgaaagagca gtatctcaag tatcagaaca gtgagcgtct ggtctgtcgt
     3541 gagtgttctg ccgaagtcga acagcagatg cgccccacct ggcacatcgt tggcaaccgc
     3601 attttcccga aaagttcgct gagccgttaa ccagactcgc tgctctgcgc actctcaccg
     3661 cgccgccatc ccccagttac gggcagtttc gctcgccatt gcaggcatcg gtgagaccac
     3721 cagcttcggc aaggcactgg cgcacttcgt ccgaagccaa ttggcgagga aagttgtaga
     3781 gaatcccacc gggacgttcg ttgtaatacg ggcggttaca gtccgggcag ccggcagttc
     3841 gaaaagcctc tccatcggcc agacagcgtt catactcatc tgccgccaac ggaactctgc
     3901 tgaggcagtc gttctcaaat cggcacgact tgtactcaag catcttgaga ctgcgcagat
     3961 atcggaagat ctgcattcgt ctccaggctg ccagatcagg aggctgcaga ctggtctgtg
     4021 cccctctgac cggagtcaga gcaaacaaac cgacctttcc gtgatgcgcg taaacccagt
     4081 cgatcgtctg ggtaaactcg cgttcggtat cgcccaaacc gaagatgagg tgaattcc
//
LOCUS       DAORHD010000287         4133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23338_length_4133_cov_2.884747, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000287 DAORHD010000000
VERSION     DAORHD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23338_length_4133_cov_2.884747"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1100)
                     /gene="deoC"
                     /locus_tag="PKO06_07375"
     CDS             complement(402..1100)
                     /gene="deoC"
                     /locus_tag="PKO06_07375"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNV69498.1"
                     /translation="MDKGHDSPLPARELARFIDHTLLKATATTAEITKLCEEARQFEF
                     ASVCVNPANVALAAKLLKGSPVKVCTVIGFPLGATSTFTKVMETRDCVANGATEIDMV
                     INVGALKSKDYELVKNDIARVVEAAGGNIVKVILETSLLNEEEKIKGCELSKAAGADF
                     VKTSTGFSTGGATVDDIKLMRRVVGPSMGVKASGGVRDTKGAQEMIAAGATRIGASAS
                     VGIVKGTAAEGKGY"
     gene            complement(1928..2236)
                     /locus_tag="PKO06_07380"
     CDS             complement(1928..2236)
                     /locus_tag="PKO06_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNV69499.1"
                     /translation="MQIAKVVKRVVSTVKNRAFDQKPLLLVQPLDISLQHKGAEVLAI
                     DFMGVDQGEIVLLMKEGSSVQQMLGNKDAPADAAIVGVIDTIDCDDRTVFEKYPSNPD
                     "
     gene            complement(2239..2499)
                     /locus_tag="PKO06_07385"
     CDS             complement(2239..2499)
                     /locus_tag="PKO06_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HNV69500.1"
                     /translation="MYLGKVLGRVVCTQKDPQMQGLKLLIVTAIDGEGKPRGKPFIAV
                     DSSGAGAGETVFLCGGGEAALPFARQRPPSDATILGIVDQIE"
     gene            2834..2907
                     /locus_tag="PKO06_07390"
     tRNA            2834..2907
                     /locus_tag="PKO06_07390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2868..2870,aa:Pro,seq:ggg)
     gene            3158..>4133
                     /locus_tag="PKO06_07395"
     CDS             3158..>4133
                     /locus_tag="PKO06_07395"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV69501.1"
                     /translation="MKHSISVGPDTTCIHAGQEPDRLFGGVSVPIYQSSTFAFESAAQ
                     GAARFSGADAGYKYTRLGNPTTAALEACVAELEGGGFGMATASGMAAISTVFMALLGN
                     GQHIVSTDSVYGPTRTILEREFSRFGVESSFVDTSDLGNIKKALRPNTKIVYLETPAN
                     PTLSISDIAGAAKIAKAHGALLVVDNTFCSPLLQKPLDLGADIVLHSMTKFLNGHSDV
                     VAGMLVARSQEVFQKLKGVLVQMGGTIDPHQAWLVLRGIKTMGMRVKTAQDNALKLAP
                     DLAKHPAVAWVKYPGLPNHPQFELAKKQMKGPGAMISFELKGGVEAGRQ"
BASE COUNT          835 a         1250 c         1146 g          902 t
ORIGIN      
        1 ggatccttac ctgcaccacg atcgctctgc tgatccggga ggtccgtttt catgcttaaa
       61 cgcgtcattc tgtttgggtt cgtgtccctt ttctggacct tcgccctctc cggctgtttt
      121 taatccgtca ccgagatcaa ccgatgcggc aggcgattcc atgcgctttt tcttgcccag
      181 agcagaaaag aaccgcaccc tgtcctcatt ctcggcaaag gcgctcaccc gacccacagg
      241 atgtgggaag tgggcttttg ctgccaagga gggcggtccc aaacgaagtt tgggacatgc
      301 aaacgacccc tgctggcgct cagctagaaa acacgcatac tcgtggatcg cgaagtcact
      361 cgctcgtgcg cgtgagctcg agttcgaccg gaggtttgga ctcagtatcc cttgccctcg
      421 gcggccgtcc ctttcacgat tccaaccgag gcactggccc cgatacgtgt cgcgcccgcg
      481 gcgatcattt cctgcgcgcc tttggtgtcg cgcactccgc cgctggcctt gactcccatg
      541 gaagggccga cgacccggcg catcaacttg atgtcgtcaa cggtcgcccc gcccgtgctg
      601 aacccggttg aggttttcac gaaatccgcc ccggcagcct tggaaagctc gcagcctttg
      661 atcttctctt cttcattgag caacgacgtt tcgagaatca ccttgacgat gttgcccccg
      721 gcggcttcga cgacacgggc aatgtcgttc ttcaccaatt cgtagtcttt gctcttgagc
      781 gcgccgacat taatgaccat gtcgatttcg gtggcaccgt tcgccacgca gtcgcgggtt
      841 tccatgacct tggtgaaggt cgacgtggca ccgagtggaa agccgatgac ggtgcagacc
      901 ttcacgggag aacccttcaa cagcttagct gcaagagcca cattcgccgg attgacacag
      961 accgaggcaa attcgaactg tcgggcctct tcacacaatt tggtgatttc cgccgttgtg
     1021 gctgtcgcct tcaacagcgt atgatcaatg aagcgggcca gttcacgtgc gggaagcggt
     1081 gaatcatgcc ctttgtcgat gcgggccgcg ccggcatcga gaattttctt aaccgagtgc
     1141 tcacacgaac tggtacaact cccgtagctg cgcgggcagt tttcctgcag acacccggag
     1201 ccggccgatg ccggagctcc ggcgtgaacc cccgacgcag gttgcgaggc gctcggcgcg
     1261 gccgcgtcag aagcgcaagt gacgcagcca cccggcccga cgcaggtggt acacttatgg
     1321 tctggcttga gattcagcgc gccacgctga aaccgcacgc ccatggcctc gagatctcgt
     1381 cgcaacccgt cgattcgtcg cgccacgcca ctcggaaagt accgggacgc atcaagcaaa
     1441 gattcgtcgc tgaccgtaat aggaatacca tcgatgagcg cgtaggaaat cacctgcgtg
     1501 gcatagctgt cggcaatcag gttggccgcc ttcgacaagg tgttgatcga aagcgatgga
     1561 atcaacagtt cccggacatc tttcatcgcc tgatgcgccg gaagattgtg tgaagagtag
     1621 atttccgtgc ccgcatttcg gcatgcgctc agcagattct gatggagata ctcggacacc
     1681 agcagtttgc cgttcactgg gataaccccc gaaatctgcg ccagcacctg atcgacaacc
     1741 ttgaagttcg ccgtgatcag cacagccgtt cggtcgcgac cggcaaccga ctttgttgcc
     1801 gcgggttgag gcggagtgta tgctcctccg cggttggccc catccgcttt cagcttcagc
     1861 atgacttcac gagcgatttt ttccacgatg ctgtccatat ccggttctcc ttcgggtcga
     1921 cggaagttta gtcagggttc gacgggtatt tttcgaagac cgttcggtcg tcacaatcaa
     1981 tcgtatcaat gacgccgaca atcgcggcgt ccgcaggcgc atccttgttg ccgagcatct
     2041 gctgaacaga actgccttct ttcatgagca gcacgatctc accctgatcg acgcccatga
     2101 aatcaatcgc cagaacttcg gcccctttat gctgcagcga gatatcgagc ggctgcacca
     2161 gcagcagcgg cttctggtca aaggcacggt tcttcaccgt cgaaaccacc cgcttcacga
     2221 ctttggctat ttgcatggtt attcgatctg gtcaacgatg cccagaatgg tggcatcgga
     2281 aggcggccgc tgtctggcaa agggaagggc ggcttcacca ccaccgcaca ggaatacggt
     2341 ttcgcccgcc ccggccccgg aggaatcaac cgcgatgaac ggtttcccgc gaggcttacc
     2401 ctcaccatcg atggcggtga cgatcagcag tttcagaccc tgcatctgcg ggtctttctg
     2461 ggtacagacc actcggccca ggactttacc gaggtacatt cccttcccct gcgtgagatt
     2521 tcggtcaact gtagcagaac gccttctcaa acgcaacttg tacaagacag tgactggcat
     2581 tctcggcacc gcctcaagga cccgcgatgc gaggtgcgct gtcgccacgg tgtgttgtat
     2641 tgcgcgatcg cgaggaacaa cggtggtcgg gtcgcagggc acaaccggtg ctgacccggc
     2701 caatgtcggt tcgagaaatt ctctgcgtcg aggtcgatac caagccgttt ttgcacgccc
     2761 cgcccagaac atcgggagtt ttctattgcg atggatcggc gtttatgcta tagtaaccct
     2821 cgggtatcac ccacggggcg tggcgcagtt ggctagcgta cttgaatggg gttcaagtgg
     2881 tcgccagttc gagtctggcc gccccgaccg aaaaaacagg cttgtcctct gacaagcctg
     2941 tttttctttg tcacggctct ttcagccttc tctccagaag ccgctttcaa accatatctg
     3001 atccttcatc ccagaggaac cgaccatttc cagcgtaacg aacgagaagg gctgcagtat
     3061 cagcagttca tcgcgacagg agagatggac aaacacgtcg gcgggcatta caatgcgccg
     3121 aaaagtcctc gatacctgga aaggagatct ctcattcgtg aagcattcca tttctgttgg
     3181 tccggacacc acctgtattc atgctggcca ggagccagac cgtctcttcg gtggtgtcag
     3241 cgtccccatc taccagtcat caaccttcgc tttcgaaagc gctgcccagg gcgcagcgcg
     3301 tttcagcggt gctgatgccg gctacaagta cacccgcctg gggaatccga ccacggcagc
     3361 tcttgaggcc tgcgtcgccg agcttgaagg cggtggcttc gggatggcaa cagcttccgg
     3421 catggcggct atttccacgg tattcatggc ccttctgggt aacggtcagc acatcgtttc
     3481 gaccgattct gtctacggac cgaccagaac cattctcgag cgcgagttct cgcggtttgg
     3541 tgtcgagagc agttttgtcg acacctcgga tctgggcaac atcaagaaag ctctgcgacc
     3601 caacaccaag atcgtctatc tcgaaacccc ggcaaatccg accctttcca tctctgatat
     3661 tgccggtgcc gcgaagattg ccaaggctca cggggcgttg cttgtcgttg acaatacctt
     3721 ctgcagccca ctgctgcaga aacctctcga tcttggcgca gacattgtcc tgcacagcat
     3781 gaccaagttc ctcaacgggc atagcgacgt cgtggcggga atgttggtcg ctcgctcgca
     3841 ggaagtgttt cagaagctga agggcgttct ggtgcagatg ggcggcacca tcgacccaca
     3901 tcaggcgtgg ctggttctgc gtgggatcaa aaccatggga atgcgcgtca agaccgccca
     3961 ggacaatgcc ttgaagctcg ctcccgacct ggcgaagcac ccggctgtcg cctgggtgaa
     4021 ataccccggg ctgcccaatc acccgcagtt cgagctggcc aagaagcaga tgaaggggcc
     4081 gggcgccatg atctccttcg aactcaaggg cggtgtagaa gccggtcgac agc
//
LOCUS       DAORHD010000288         4132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23341_length_4132_cov_4.041697, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000288 DAORHD010000000
VERSION     DAORHD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23341_length_4132_cov_4.041697"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PKO06_07400"
     CDS             <1..754
                     /locus_tag="PKO06_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV69502.1"
                     /translation="IASPFGLVVMAGPYGSGKTTTAYHALQQLRENGRVNLATVEAPY
                     EMILPGVAQTHVRPHLGLTFPALLAAIERGDPDVIYVSDIPDEDTMSRVLKLALTGHL
                     IFVTMHASSVEEVIGKLLDLKAPAHLLASALTGIMIQHLARKVCSACAHAVKLPATAL
                     KPYKLGKVTTSGREGKGCATCNQSGYRGRMPLYEIFTPDKSFKDTMQTGDRSGIRRAL
                     QIPPRRTLAVAAAEAVAAGLTTLAELDRVTNS"
     gene            complement(812..2071)
                     /locus_tag="PKO06_07405"
     CDS             complement(812..2071)
                     /locus_tag="PKO06_07405"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNV69503.1"
                     /translation="MPTFLVQSLGCKVSQYDGQRLEEHLRTLGFVPVAGDQEPDLFIL
                     NSCSVTGRAAQKVRQALGAVRRRWPAAKLVLAGCEAKNRDLAAQSSPEADGVLLPNST
                     LALVEELIAGLGFALSGAATAAFDHTPIAKQRTRAFLKIQDGCRQFCAYCIVPHLRGD
                     EVSKSISDAVQEARQLVQNGFRELVITGIHLGRYAPGLVPFLREIETISGLDRIRLSS
                     IESVEVGDDLIDWLATSVKACAHLHLPLQSGSDEILRAMNRPYTTADFQNVVERLRRR
                     LPDIGITTDFMVGFPGESDALFAESLRFAEMIGFSRMHIFRYSPREGTPAATMCQQVD
                     GRVKRQRAERAEEVWRRSALAFHRRFIGRTVEVLWETCIDNIWRGMSREYIPCESADH
                     PCVDLTNTIQRVNVQLADQENVVVRSL"
     gene            complement(2071..2790)
                     /locus_tag="PKO06_07410"
     CDS             complement(2071..2790)
                     /locus_tag="PKO06_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNV69504.1"
                     /translation="MTKTHDRRRVPDARVYEPDFDGVLSEANMHYLERVLRLVPGDRF
                     CVTDGRGREGWSEFSADGALLVREIQEPDRDCRWRVTLFAGLTKGDRFEWLLEKAVEL
                     GVTCVVPLVSARCVVRELSAAKLDRYRKIALAAMLQSGGCWLPRIEDPQPVAHLAEPH
                     GNVCGWLLHEGFGGGGSLPAVETNHDEIWVCSGPEGGFTESEVETLLARGWRGVSLGP
                     RRLRAETAPLVAVSALLLTRA"
     gene            complement(2930..3511)
                     /gene="rsfS"
                     /locus_tag="PKO06_07415"
     CDS             complement(2930..3511)
                     /gene="rsfS"
                     /locus_tag="PKO06_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNV69505.1"
                     /translation="MAITKKNLVPKKTAKSILADKAKKSKKTTTKKKIVRLPRASVPE
                     TSGKGAGTAKAKGLKLADVPELLQKAVRLLVEKKGENIILMEVSELVGYTDYFLLATG
                     NSTPQVQAMAEAVAFEVKHAGKPTRIEGKQDAVWVLIDGGDFVVHLFQPEARKFYSLE
                     DLWGDACLIEIDEQEILAPKRKTKKAKTAATED"
     gene            complement(3516..4112)
                     /locus_tag="PKO06_07420"
     CDS             complement(3516..4112)
                     /locus_tag="PKO06_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV69506.1"
                     /translation="MSTNIERIILAGFGGQGILFLGKVLAEVGMHSGKHVSWIPSYGP
                     EMRGGTANCTVVVSENPIASPMVTIPDTVIALNKPSVAKFSPRIKGGGMLMYNSSLIE
                     RQEFRDDIRLVEIPASEIADDLGNPRVANMVMAGAYAKYSKVITYDDLVRLLPSLLPA
                     KKKELIEINMAAVERGFSFVKEKKYMFGRYVYSVFKGQ"
BASE COUNT          875 a         1299 c         1112 g          846 t
ORIGIN      
        1 gatcgcctct ccgttcggcc tggtggtcat ggccggtccg tacgggtcag gcaagacgac
       61 aaccgcctac catgcgttgc aacagttgcg cgagaatggc cgggtcaacc tcgcgacggt
      121 cgaagcgccc tacgagatga tactgcccgg agtcgcccag acgcatgtgc ggccgcatct
      181 tggtctcacc ttcccggcgc tgctggcggc aatcgaacgc ggagacccag acgtcatcta
      241 tgtcagcgat attcctgacg aggacacgat gagccgggtt ctaaaactgg ctctgaccgg
      301 ccacctgatc ttcgtcacca tgcatgcctc gtcggtcgag gaagtcatcg gaaaactgct
      361 ggacctcaag gctccggcgc acctgctggc atcggccctg accggcatca tgattcagca
      421 tctggcgcgc aaagtctgct ccgcctgtgc ccacgcggtc aaactgcccg ccacggctct
      481 gaagccctac aagctcggca aagtcacaac gagtgggcgc gaaggcaaag gctgcgccac
      541 ctgcaatcaa agcggatacc gtggccggat gcctctctac gagatcttca ccccggacaa
      601 gtcattcaag gatacgatgc agaccggcga tcgttctggc attcgccgtg ctctacagat
      661 tccgccgcgt cgtaccctgg cggttgccgc cgccgaagcg gttgccgccg gcctcaccac
      721 cctcgccgag cttgaccgtg tcaccaacag ctaatgtcac gggaatctct cactcccccg
      781 tctggctaat ccagtgccag atggggtgag gtcacaggct tcgaacgaca acgttctcct
      841 gatcagcgag ctgaacgttg actcgctgaa tggtattcgt caggtcaacg cacggatgat
      901 cagcagactc acacggaatg tactcgcgac tcatgccgcg ccagatgttg tcgatacagg
      961 tctcccacag aacttcgacc gtgcgaccga tgaagcgacg gtggaaggcg agggcggaac
     1021 gacgccacac ttcttcggcg cgctcggcgc gttgccgttt cacacgaccg tctacctgct
     1081 gacacatcgt ggcagcaggt gtgccttctc gcggcgaata gcggaaaatg tgcatgcgtg
     1141 agaatccgat catctcggcg aaacgcaagc tctcggcaaa caaggcatcg ctctcgcccg
     1201 ggaacccgac catgaagtcg gtcgtgatgc cgatatccgg caggcgtcgg cgcaaccgct
     1261 cgacgacatt ctgaaaatcg gcggtcgtgt aggggcggtt catggcccgc agaatctcat
     1321 cactccccga ctgcagcggc agatgcagat gagcacaggc tttcacagag gttgccagcc
     1381 agtcgatgag atcgtccccc acctcaaccg actcgatcga acttaaccgg atgcgatcca
     1441 agcccgagat tgtctcgatc tcgcgcagga acggcaccaa tcctggcgca taccgaccga
     1501 ggtgaatgcc ggttatgacc agttcgcgaa acccgttctg tacgagttgt ctcgcttctt
     1561 gcactgcatc tgatatcgat ttcgacacct catctccgcg caggtgcggc acaatgcaat
     1621 aggcacagaa ctgccgacag ccatcctgaa ttttcagaaa agcgcgcgtt cgttgcttcg
     1681 caatcggcgt atgatcaaac gctgccgttg cagctcccga cagagcaaac cccagcccgg
     1741 caatcagctc ttcgacgaga gccagcgttg agttcggcaa cagcacgcca tcggcttccg
     1801 ggctgctttg tgctgccaga tcacggttct tcgcctcgca cccggcgagg accagtttcg
     1861 ccgccggcca gcggcgcctg accgccccga gggcctgacg aaccttctgc gcggctctgc
     1921 cggtaactga gcagctattg agaatgaaaa gatcaggctc ttgatcgcca gcgactggta
     1981 caaatccgag ggttcgcaga tgctcttcaa ggcgctgtcc gtcatattga ctgactttgc
     2041 atccgagaga ctgaaccaga aaagtcggca tcacgcgcgt gtgagaagca aggcggagac
     2101 ggcaaccaag ggtgcggttt ccgcgcgcag gcgacgcggg ccgagagaca cgccccgcca
     2161 gccacgggcc aacaacgttt cgacctcact ctccgtgaat ccaccttctg gaccgctaca
     2221 gacccagatt tcatcatgat tcgtctcgac cgccggcagg cttccgccgc cgccgaagcc
     2281 ttcgtgcaga agccaaccac agacgttccc gtgcggttct gccaggtgcg cgacaggctg
     2341 tggatcttcg atccgcggaa gccagcagcc tcccgactgc agcatcgccg ccagcgcaat
     2401 cttgcgataa cgatcgagct tcgcggcaga cagttcgcgc accacgcacc gggcggaaac
     2461 gagcggcaca acgcaagtga ccccgagttc gaccgccttt tccagcaacc actcaaaacg
     2521 atcccctttg gtgagccccg caaacagcgt cacccgccat cggcaatcac gatccggttc
     2581 ttgaatctcg cgcaccagaa gagccccatc ggcagagaac tcactccatc cttcacgacc
     2641 tcgcccatcg gttacacaga accgatctcc cggcaccagt cgcagtacac gttcgagata
     2701 gtgcatattc gcttcactca aaaccccgtc gaaatccggt tcatacaccc gagcgtccgg
     2761 tactcggcgg cgatcatgcg ttttcgtcat gcaggtattc gtttcccaat gccgattctt
     2821 ggccgttcgc cttcagcggc gaatcgaccg gaagcctgaa aacaaacgaa cattgagggc
     2881 aaatgaaaaa cccgccggct cccggtgagg ttgcaggcgg gttcacagat caatcttcgg
     2941 tggcggccgt tttcgccttc ttcgtcttgc gcttcggggc gagaatttct tgttcatcga
     3001 tctcgatcaa acaggcatca ccccagagat cttcgaggct gtaaaacttg cgggcttccg
     3061 gctgaaagag atgcacgacg aaatcgccgc catcgatcag tacccagacg gcatcctgct
     3121 tgccttcgat gcgggttggc ttgccggcat gcttcacttc aaaggctacg gcctcagcca
     3181 tggcctgcac ctgtggcgtg ctgttcccgg tggccagaag aaagtagtcg gtatacccga
     3241 ccagctcaga gacttccatg agaatgatgt tctcgccctt tttctcgacc agcagtcgca
     3301 cggctttttg cagcaactcg ggaacatccg cgagcttgag tcctttcgcc tttgcagtac
     3361 cagctccctt gccggaagtt tcaggcacag aggctctcgg aagccgaaca atcttcttct
     3421 tcgtggtcgt cttctttgac ttttttgcct tgtctgccag tatcgacttg gccgtcttct
     3481 tcggaaccag gttctttttc gtgattgcca tggcgttact gacctttgaa tacggaatag
     3541 acgtatcgac cgaacatgta cttcttttcc ttcacaaacg agaacccgcg ttcaaccgcg
     3601 gccatgttga tttcgatcag ttcttttttc ttggcaggaa gcagcgaagg cagcaatcgt
     3661 accaggtcgt cataggtgat gaccttgctg tatttggcat acgcgcccgc catgaccatg
     3721 ttcgcaaccc gggggttccc cagatcatcc gcgatctcgc tggccggtat ctcgaccaga
     3781 cgaatatcat cgcggaactc ctggcgctcg atcagtgaag agttatacat cagcataccg
     3841 ccgcccttga tccgagggct gaacttggcc accgaaggct tgttcaaggc gatgacggta
     3901 tcgggaatgg tcaccattgg tgaagcaatc gggttttccg aaaccacgac ggtgcagttg
     3961 gcggtgccgc cgcgcatttc cgggccatag ctgggaatcc aggagacgtg tttcccggaa
     4021 tgcataccga cttctgccag caccttgccg agaaagagaa ttccctgacc accgaaaccg
     4081 gcgagaatga tacgttcaat gttggttgac atcggtttcc tccaggctca ca
//
LOCUS       DAORHD010000289         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23425_length_4121_cov_3.490408, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000289 DAORHD010000000
VERSION     DAORHD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23425_length_4121_cov_3.490408"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PKO06_07425"
     CDS             complement(<1..478)
                     /locus_tag="PKO06_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNV69507.1"
                     /translation="MTTLEQAIQALKANGFTARLHADRHQAVQAVLAEIPDKVSIGRC
                     GSVTCTELGLYDRLVHRGNLLIDPYQPHLTPEEKDATRRKTQQSDVLLTGTNAVTLDG
                     KLVNIDGVGNRVTAQVFGPGHVIIVIGRNKLVTDVHAAIRRIKTIACPLNAKRLKLE"
     gene            648..1064
                     /locus_tag="PKO06_07430"
     CDS             648..1064
                     /locus_tag="PKO06_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV69508.1"
                     /translation="MKSYRKELWFNTPTRRAFVNITPQIEECLRESGIREGLVLCNAM
                     HITASVFINDHESGLHHDFEVWLEKLAPEKPHSQYRHNGGEDNADAHLKRQVMGREVV
                     IAVTDGKLDFGPWEQVFYGEFDGMRRKRALVKIIGE"
     gene            complement(1068..2483)
                     /locus_tag="PKO06_07435"
     CDS             complement(1068..2483)
                     /locus_tag="PKO06_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69509.1"
                     /translation="MRLSLDNWVQGQFHHERRDHASRRRKVIVLLLLFSLAICLTAYP
                     EPSPATYPAYVASTAFSLNWYVLNFSGSSETRDISTLVDHRGHIDVTRAITLPHGSCT
                     WTLAEPNEPGLYIFSGSDHPASDTIWLYAEFELATDTPLLWWYDADDLVHVWVNGRDL
                     GHKPFPTGVATPALATLFAGRAGKNALLARVDNVVGVLTFFTELRAVTSPATLQTDIT
                     ALCQELLAPSHLDTLLKVNEINRREPFDQEILNYFESWNQDLPRCPLAKIAAAFRLAS
                     QIPSRHQQRRQAHFLRWLIDQSGVQTELDRQLSEGLRRIRISRNVTPNLARRLFARRL
                     YPEGVLATRLLRATGTMPGDLPSLMFRALFSRFQFGLVWETSMEIESLTRLLDPRDRI
                     LVQYQAALHDFLTTDVASAAEKLDQICRENPDFEPAFHLRSVSLLQHFPPPPQRSEKA
                     FKEWRQRAQEADLRLYRSLIR"
     gene            2682..>4121
                     /locus_tag="PKO06_07440"
     CDS             2682..>4121
                     /locus_tag="PKO06_07440"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV69510.1"
                     /translation="MISTPNNNDPLHQTETVTTTVEPLLAADTPATELVQTPDPFPPF
                     RFEDLPETLLRAVRDCGWNEPMPVQARIIPWLLNKRDIIVQSQTGSGKTGAFVLPIMQ
                     MIDPTQPVTQALIMAPTRELAQQVKTECDRLGAHHGVRSVAVYGGVGYVPQIEAFKRG
                     AHIVVGTPGRLLDHLNRRNLSLAKVQVLVVDEADELLSMGFWPDMRRIRSYLPKNRIS
                     ALFSATIPGGVRVMADELLHQPQFLGLSSDTVHVTDMDHIYYVVDAMQKDRVLMRILE
                     VENPSSAIIFCNTRSEVEYLSAFLRRFGYDVDQISGDIGQRDRESVMKRLKDHTLRLM
                     VATDVAARGIDVSRLEYVFIYDFPPDVEQYVHRAGRTGRAGNRGVAVSLVSVIQEGDV
                     KRCARRHGIPFIKKNTPTPEEVQNRLAERLMARLEAELRDLDTAGRERMKGFQRLLSQ
                     IQEHEQAADLLLMLLDDKAARLRAEGRPEP"
BASE COUNT          882 a         1094 c         1302 g          843 t
ORIGIN      
        1 tctcgagttt caggcgcttg gcattcaacg gacaggcgat ggttttgatg cggcgaatgg
       61 ccgcgtgaac gtcggtgaca agcttgtttc gcccaatgac gatgatgaca tggccggggc
      121 caaacacctg cgcggtgacc cgattcccga caccgtcgat gttgaccagc ttgccgtcga
      181 gggttacggc gtttgtaccg gtgagcagca cgtctgactg ctgtgttttt cgtcgtgttg
      241 cgtctttttc ttcaggagtc aggtgcggct gatacggatc gatcagcagg ttgccgcgat
      301 gcaccaggcg atcatagagt ccgagttccg tgcaggtgac tgagccgcag cgcccgatcg
      361 agaccttgtc gggaatctcg gcgagcacgg cttgtaccgc ctggtggcga tcggcatgca
      421 gtcgggcggt gaatccgttg gccttcaggg cctgaatcgc ctgttcaagt gtcgtcatct
      481 cgtcttcccc ctgtctgaat gtggcgacgc cgggtgtggc gcacctctgg tcggagttca
      541 gtatacgcgt tcgcaaccca cacgcaaagg ggaccctggg gtgtgctata gttgaggcag
      601 ccggtacgcg tgccggctca gatgcagaac agggcgagga ggcagtcgtg aaaagttacc
      661 gcaaggaact ctggttcaat accccgacgc gacgagcgtt cgtcaacatc accccacaga
      721 tcgaggagtg tctgcgggaa agcggcattc gtgaagggtt ggtgttgtgc aatgccatgc
      781 acattacggc cagcgtgttt atcaatgatc acgagagcgg gctgcatcat gatttcgagg
      841 tgtggctcga aaaattggcg ccggaaaaac cccattcgca gtatcgccac aatggcggag
      901 aagacaatgc cgacgcccac ctgaaacggc aggtgatggg acgggaagtc gtgatcgcgg
      961 tcaccgatgg caagctcgac ttcggaccct gggaacaggt gttttatgga gagttcgatg
     1021 gcatgcgccg caaacgcgcc ctggtcaaga tcatcggcga gtgacgatca gcggatcaga
     1081 ctgcgataga ggcgcaggtc ggcttcctgg gcgcgttgcc gccactcctt gaaagctttc
     1141 tcgctgcgct ggggcggcgg cggaaagtgc tgcagcagac tgacgctgcg caggtgaaac
     1201 gccggttcaa agtcagggtt ttcgcgacag atctgatcaa gtttctctgc ggccgaagcg
     1261 acatcggtgg tcagaaagtc gtgcagtgcc gcctgatatt gaacgagaat gcggtcgcgc
     1321 gggtcgagca gacgcgtgag agactcgatt tccatggaag tttcccacac aagcccgaat
     1381 tgaaatcggc tgaagagcgc gcgaaacatc agggaaggca agtcgccagg catggtgccg
     1441 gttgcccgca gaagtcttgt ggcgagcacg ccttcggggt agagacggcg cgcaaacagg
     1501 cggcgtgcca gattcggcgt gacattccgg gagatgcgga tacgccgcag accctcggag
     1561 agttgtcgat caagctctgt ctgaacacct gattggtcaa tcaaccaacg aagaaagtgt
     1621 gcctgtcgac gctgttgatg acgtgaggga atctgtgagg cgagacgaaa tgccgcggcg
     1681 atttttgcca ggggacagcg cggcagatcc tgattccagg attcgaagta attcaggatt
     1741 tcctggtcga acggttctcg gcgattgatt tcgttgacct tgagtaatgt atcgaggtgg
     1801 ctgggggcca gcaactcctg gcagagagcc gtgatatcgg tctgcagtgt ggcaggggag
     1861 gtgactgcgc gaagctcagt gaaaaacgtg aggacgccaa cgacgttgtc cacacgcgcc
     1921 agcaacgcgt ttttccccgc ccgcccggca aaaagagttg ccagcgcagg agtcgcaacg
     1981 cctgtgggaa acggtttgtg tccgagatcc ctgccattga cccacacgtg aaccagatcg
     2041 tcggcatcat accaccagag gagcggggtg tcagtagcca gctcgaattc tgcatacagc
     2101 cagatcgtgt ctgaagcggg gtgatctgaa ccggagaaaa tgtaaagccc cggctcgttc
     2161 ggttccgcga gagtccaggt acatgagccg tgaggcagtg tgatggcgcg ggtaacatcg
     2221 atatgccccc gatgatcgac aagagtggaa atgtcgcggg tctcagagga acccgaaaag
     2281 ttgaggacat accaattcag agaaaaagct gtcgaagcca cgtaggcagg ataggtggcg
     2341 ggtgatggct cgggataggc ggtcaggcag atcgcaagag agaagagcag aagaaggaca
     2401 atgaccttcc tgcgccggga cgcatgatct ctgcgctcat ggtggaactg gccctgcacc
     2461 cagttgtcga gggatagacg catgcccaca ttctgacaga ctgtgaagct actgtccatc
     2521 ctgcgaatga cgtcggtcag gaaatgtgtc tccccagaat ttccccgggg cgggattgac
     2581 gaatgagccg gaatgcacta ctctgcgggc attggaagcg gcgactgaaa gagaggtcgc
     2641 cccccgccga atacggcgga ttcttgaccc ggagcctcgc gatgatatcg acacccaata
     2701 acaatgatcc ccttcaccag actgaaacgg tgacgacaac cgtggaaccc cttctggctg
     2761 ctgatacgcc ggcaacggaa ctcgtacaga caccagaccc attcccgccg tttcgttttg
     2821 aagacctgcc ggaaaccctt ctccgagccg tgcgtgattg cggctggaac gagccgatgc
     2881 cggtgcaggc gcgaatcatc ccctggttgc tgaacaaacg cgatatcatc gttcaatcac
     2941 agaccggaag tggcaaaacg ggagcgttcg tcttgcccat catgcagatg atcgatccaa
     3001 cccagccggt gacgcaggca ttgatcatgg ctccgacccg cgagctggcg caacaggtga
     3061 agaccgaatg tgatcgcctg ggagcgcatc atggcgttcg ctcggtggcc gtctatggtg
     3121 gcgtcgggta tgtgccgcag atcgaggcgt tcaagcgtgg ggcacatatc gtggttggta
     3181 cacccggccg tttgcttgat catctgaatc gccgcaatct ttcgctggcg aaagtacagg
     3241 ttctcgtggt tgatgaggcc gatgaactct tgtctatggg gttttggccc gatatgcgcc
     3301 gcattcgttc ctatctgccg aagaaccgta tctcggcact gttttcggcg acgattcccg
     3361 ggggtgtccg agtgatggcc gacgaattgc tgcatcagcc gcaatttctc gggttgtcgt
     3421 cagatacggt ccatgtcacg gatatggacc acatctacta cgtggtcgat gcgatgcaga
     3481 aagaccgcgt tttgatgcgg attctcgagg tcgagaatcc ttcgagtgcc atcatcttct
     3541 gtaatacgcg cagcgaagta gagtatctct ccgccttttt acgccgcttt gggtatgacg
     3601 tcgatcagat cagcggggat atcggccagc gcgatcgcga gtcggtgatg aagcggttga
     3661 aggatcatac gctcaggctg atggtggcga ccgatgtggc cgcgcgcggc attgatgtca
     3721 gtcgtctcga gtatgtcttc atttatgatt ttccgcccga tgtcgaacaa tatgtgcatc
     3781 gggctggtcg taccggccgt gccggcaacc gcggcgtggc ggtcagcctg gtttccgtga
     3841 ttcaggaagg cgatgtcaaa cggtgtgccc gccgccacgg aattccattc atcaagaaga
     3901 acacgcccac ccccgaagaa gtccagaacc gactggctga gcggttgatg gcccgcctgg
     3961 aggcggagct gcgcgacctc gataccgccg ggcgcgagcg catgaaaggc tttcagcgac
     4021 tgttgtctca gattcaggaa catgagcagg ccgctgatct cctgctgatg ctgctcgatg
     4081 acaaggctgc gcggctgcgt gccgaaggcc gcccggaacc g
//
LOCUS       DAORHD010000290         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23432_length_4120_cov_3.714391, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000290 DAORHD010000000
VERSION     DAORHD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23432_length_4120_cov_3.714391"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1458
                     /locus_tag="PKO06_07445"
     CDS             166..1458
                     /locus_tag="PKO06_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69511.1"
                     /translation="MSPKHPLAKDSAQSPWHIGGVIVLILVLGFSIRLSRAPTQVVTP
                     PGVPPALPLTQGQTYPTPYNPVPSAVPTPVVNTPTPVPSVAAPVIPAVQPTVPGQQVA
                     APTTQGSSNVPVPAVSNSLWKAIFPLNAVNAILALPDGTTWVAHEQGLARIIQNGMVN
                     FTFQNRTFPSVNATSLAYDGRNVWVGTFDGLVQVSDGNTFRRFTTRDGLIHDMVWSLD
                     WDGHILWIGTQAGVSFMTADGRFERIDKRISNGGLADIWIGAVRKIDRWVLCGNDDGL
                     SIWDTAGFAANPGSWVTLDQFETNLVHNWVLGMTRYQDKLWIGTPQGLARLETSFDQL
                     FQPGSSDKTSGPRSLFSTFNRSTGLPSDRVNALQPWKDALWLGTQAGLSRWQNGAIRT
                     WTEADGLLANDVRAVADGGEGLWVGTSRGLQLLSATAL"
     gene            1503..3521
                     /gene="ligA"
                     /locus_tag="PKO06_07450"
     CDS             1503..3521
                     /gene="ligA"
                     /locus_tag="PKO06_07450"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNV69512.1"
                     /translation="MDLFSIQDRIDELRRTIARHERLYYVEAAPEVSDAEFDRLMNEL
                     KALEAAYPHLVTADSPTQRVGGAVTGFHPVTHRVPMMSLDNAYSLGDLHDWVQRMQKL
                     ASRPVFPLVAELKIDGVSLSLHYAQGRLQAAATRGDGTSGDLVTENVRTIRSLPLHID
                     SSLDIDVRGEVYVPRAQLLEINRERLAQGEDPFKNCRNLAAGTLKSLDPAVAAARKLR
                     VLTYGIAQARDCGFASHSETLDFLRQQHLPVNHPVRLCHSVDEIDAFVQEIDSAKETL
                     EFDIDGIVLKVDDLALQLELGATAKAPRWAMAYKFAHQQAVSRLEAVVWQVGRAQITP
                     VAHLQPVELGGTTVSRASLHNLDQIREKDIRIGDQVVVEKAGFIIPYIVRALPEQRTG
                     DEREIAPPEHCPSCGQPTHIETAAISASRETATVVSCPNPGCQGVLSRRLVHFVTQLE
                     IENIGPSLIDRLIQGGFLAEIPDLFRLRREELLQVERMGDKLAEKILASIDKARRAPL
                     AALIDGLGIPNVGTKVARSLAQQFRSFEALQSATAEQLSQIEGISDKISTAVTTFFGT
                     ADHHSWIQDLQKEWRGPETTETVQAPQTLVGKTFVLTGEATVPRRELEILIEQHGGRC
                     SSSVSPKTTYLLIGSLEPPEYTSAKKTKAEKLGIPIINEFDLRNISRS"
     gene            3642..>4120
                     /locus_tag="PKO06_07455"
     CDS             3642..>4120
                     /locus_tag="PKO06_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV69513.1"
                     /translation="MPLNDKLFFPKEIVCPICEKSFVRYTLRKSAFSIAKRDTDYRPI
                     YIGAINPRTYAICVCPHCYYAGEDKYFCPQLKDDDLRRKAMFDSHKAQWEAKSRVKAA
                     GSGQQIWKDLASEKLKELTPEEVAILKRITPLLQKVVAKFTGRDKPINEQQKDGDMEL
                     "
BASE COUNT          885 a         1257 c         1158 g          820 t
ORIGIN      
        1 gaatggcacg acacagagca ttcgcgagtc tctcggcaaa atcaacgatt atcagggcgt
       61 gaccgggaaa ggcggtctgg atgtcactgg gaacgaaacc cgaccggtac aactggcacg
      121 ggttcagaat ggtgccttta tccctctcac cgcgggagag gccaaatgag tcccaaacat
      181 cccctcgcta aagattccgc gcaatccccc tggcacatcg gtggagtcat cgtcctgatc
      241 ctggttctcg ggttcagcat tcgcttgagc cgcgcgccca cacaggtcgt gacgccgccg
      301 ggagtcccac cggcactgcc gttgacgcag gggcaaactt atcccactcc gtacaacccg
      361 gtcccctccg cggtaccgac accggtggtc aataccccga cgcctgttcc atccgtggcc
      421 gcgcctgtaa ttccagccgt tcaacccacc gtcccggggc aacaggttgc cgcaccgacc
      481 acgcaaggtt cgtcgaacgt accggttccg gcagtctcaa attctctctg gaaggcgatc
      541 tttccgctga atgccgtgaa tgctattctc gcgctccctg atggaacaac ctgggtcgct
      601 catgaacaag gtctggcgcg cattatccag aacggcatgg tcaatttcac ttttcagaat
      661 cgcaccttcc cttccgttaa tgccacctct ctggcgtatg atggccgcaa cgtctgggtg
      721 ggcaccttcg acggactggt gcaggtgagc gacggcaaca cgtttcgccg gttcacgacc
      781 cgtgacggtc tgattcacga catggtctgg tcactcgact gggacggaca cattttgtgg
      841 atcggcaccc aggcaggcgt ttcgttcatg accgccgatg gtcgttttga acgaatcgac
      901 aagcggattt ccaatggcgg actggccgac atctggatcg gtgccgtgcg aaagatcgac
      961 cgctgggttc tgtgcggcaa cgacgacggt ctgagcatct gggataccgc cgggtttgcc
     1021 gcgaaccccg gttcctgggt cactctcgac cagttcgaaa ccaacctcgt gcacaactgg
     1081 gttctcggca tgacacgcta tcaggacaaa ctctggatcg gcacccctca gggtctcgcc
     1141 cgtctggaaa catcatttga tcagcttttc cagccaggca gctcggacaa aaccagtggc
     1201 ccgcgttcac tcttttccac tttcaatcgc agcaccgggc tgccctcaga tcgcgtcaat
     1261 gctctgcaac cctggaaaga cgccctctgg ctcggcactc aagcagggct ttcccggtgg
     1321 cagaatggag cgatacgcac ctggaccgaa gcagatgggt tgctggccaa cgacgtgcgc
     1381 gctgtggccg atggcggcga aggtttgtgg gtcggaacct cacgcggcct gcagctgctc
     1441 agcgcgacag ccctgtagac cgtcgtagtt tctcgggaaa ccttacaggc accccctccc
     1501 acatggatct cttcagcatt caggatcgca tcgacgaact gcgccgcacg atcgcccggc
     1561 atgaacgcct gtattacgtt gaagccgccc cggaagtctc ggacgccgag ttcgatcggc
     1621 tgatgaatga gctgaaagcc ctggaagcgg cgtacccaca tctcgtcacc gctgattcgc
     1681 caacccagcg cgtcggcggc gcggtgaccg gatttcaccc ggtcacccac cgcgtaccga
     1741 tgatgagtct cgacaacgcc tattcgctgg gcgatctgca tgactgggtg cagcgcatgc
     1801 agaaactcgc ctcacgtccg gtgtttccgc ttgttgccga actgaagatc gatggcgttt
     1861 cgctttcact tcactatgcc cagggccgtc tgcaggcagc ggcaacccgc ggtgatggca
     1921 cgagcggcga tctggtcacc gagaatgtgc gcacgattcg ttccctgccg ttacacatcg
     1981 acagttcact cgacatcgac gtccggggag aggtctacgt tccgcgcgcc cagctgctcg
     2041 agatcaaccg cgaacggctg gctcagggcg aagacccgtt caagaactgt cgcaacctgg
     2101 cagccggtac gctgaagagc cttgatccag ccgtggccgc cgcgcgcaaa ctgcgcgtgc
     2161 tgacctacgg cattgctcag gcccgtgact gcgggttcgc cagtcactcg gaaactctcg
     2221 attttctgcg gcagcagcac ctgccggtga accaccccgt tcggctctgt cactcggttg
     2281 acgagatcga tgcgttcgtc caggagatcg actcggccaa agagacgctg gaattcgaca
     2341 tcgacgggat cgtgctgaag gtcgatgatc tggctctgca actcgaactg ggcgctaccg
     2401 ccaaagcacc gcgttgggcg atggcctaca agtttgcaca ccagcaggcg gtcagcagac
     2461 ttgaagccgt ggtctggcag gtcggacgag cccagatcac tccagtcgcg cacctgcagc
     2521 ccgtggagct gggcggaacc accgtgtcac gagcgtcatt gcacaatctc gatcagattc
     2581 gcgaaaaaga catccgtatc ggagaccagg tggtcgttga aaaagcgggg ttcatcatcc
     2641 cgtacatcgt gcgcgccttg ccggaacaac gtaccggcga cgaacgcgag attgccccgc
     2701 ccgagcattg cccctcgtgc ggacaaccga cccacattga aaccgccgcc atcagcgcct
     2761 cacgggaaac cgccaccgtc gtttcctgcc ccaacccagg ctgccagggg gttctttcac
     2821 gacgtctggt gcacttcgtc acgcagctcg agatcgaaaa catcggtcct tcattgatcg
     2881 atcgactgat ccagggcggg tttctggccg agattcccga cctgttccga ttgcgccggg
     2941 aagagttgct gcaggtcgaa cgcatggggg acaagcttgc agagaagatt cttgccagca
     3001 tcgacaaggc gcgccgcgcc cctctggcgg cattgatcga tggcctcggt attccgaatg
     3061 tcggtaccaa agtcgcgcgc agtctggcgc agcagttccg gtcgttcgaa gcgttgcaga
     3121 gcgcgaccgc ggaacagctt tcacagatcg aggggatcag tgataaaatc agcactgccg
     3181 tgaccacgtt cttcgggaca gcggatcatc actcatggat ccaggatctt cagaaagagt
     3241 ggcggggccc ggaaacgacc gaaaccgtac aggcacctca aactctggtc ggcaaaacct
     3301 ttgttctgac cggcgaagcc accgtaccgc gccgcgaact tgagattctg atcgagcagc
     3361 acggtggtcg ttgttcctca tcggtttcgc cgaaaaccac gtatctgctg atcggctcgc
     3421 tcgagcctcc cgagtacacg tcggcaaaaa agaccaaagc ggaaaaactc ggaattccca
     3481 ttatcaacga gtttgacttg aggaacattt cccgctcctg atcaaggatt gcggggaaag
     3541 atgggttgcg ttttcgcgat caatggtgta tggtagagag gaagggtttc tgttttgtgt
     3601 tgcttatgcg cattttcatg cagacatgaa ggggtttcca gatgccgctc aatgacaaac
     3661 tatttttccc caaagaaatt gtctgcccca tctgcgagaa atcgttcgtt cggtacacgt
     3721 tgcgtaagag cgcattcagc attgccaagc gcgacaccga ctaccggccg atctacatcg
     3781 gggcaatcaa cccacgcacg tatgcgatct gcgtttgtcc gcactgctac tatgccggtg
     3841 aagacaagta tttctgcccg caactgaaag acgatgatct gcggcgcaaa gccatgttcg
     3901 acagccacaa agcccaatgg gaagcgaaaa gccgtgtcaa ggcggccggc tcaggccagc
     3961 agatctggaa agatctggcc tctgagaaac tgaaggaact gactccggaa gaagtcgcga
     4021 ttctcaaacg gatcacgcct ctcttgcaga aagtggtcgc caaattcact ggtcgcgaca
     4081 aaccgatcaa cgaacagcag aaagatggcg acatggaact
//
LOCUS       DAORHD010000291         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23460_length_4117_cov_4.325702, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000291 DAORHD010000000
VERSION     DAORHD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23460_length_4117_cov_4.325702"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="PKO06_07460"
     CDS             <1..416
                     /locus_tag="PKO06_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69514.1"
                     /translation="MPRDQVYARMASVQREQYLQFTVKHRLANGEVRPVEVSCSLQFF
                     QGRELLHSIIHDISERCRVEQEREHLIQELQCAVAKIKTLRGMLPICAACKKVRDDKG
                     YWNQIEAFLSEFSELEFSHGICPDCKKRLYPDLED"
     gene            554..1366
                     /locus_tag="PKO06_07465"
     CDS             554..1366
                     /locus_tag="PKO06_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69515.1"
                     /translation="MDLLSVDRLKKAYQSRGREPFWAVKGISFTLGPGECFGLLGPNG
                     AGKSTSMNCITGFYQASEGAVRLMGFDVYAEPRQARQYLGVCQQEDTLDSDFNAFDQM
                     VRYASFFEIPEAEARTRTQTLLNRFGLSDKAREMVEELSGGMRRRLQVARALVSQPRI
                     LVLDEPTAGLDPEARRQLWDIITEYRAQGNSILLSTHYMEEAERLCDRVAIMHQGLIL
                     DVAPASELIAKYIGTQSVEEEIRPGLLWKRLPNLEDVFLKLTGSHLQEDSEE"
     gene            1363..2130
                     /locus_tag="PKO06_07470"
     CDS             1363..2130
                     /locus_tag="PKO06_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV69516.1"
                     /translation="MTSAKAIRLVWWRYFDVFRKNVGYSVVTTFVEPLLYLASFGFGV
                     GALVPALQLDGISVTYRQFVLAGIVAQTVLFQAFFEAAYGGFVRMYYQRIFQAIAVTP
                     ITLSEVLWGELLWNTCKSGFAAAVVVLIGCTIGDFHWRGALFILPVIILGALVFASMG
                     LLVAARSRSIDNISFPQFLLVFPMFLFCGVFFPLEQLPSTIQSLVWVLPLTPLVSLIR
                     SLMLGLPLFWPAWLILILWAAVLVPIARRAMRARLVK"
     gene            2204..2578
                     /locus_tag="PKO06_07475"
     CDS             2204..2578
                     /locus_tag="PKO06_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69517.1"
                     /translation="MYVQRMMFTARRGFTLIEIMIVVLIIGILVTFASFSYRHLRQRV
                     QRVSCRENLRILQQAAVLCQTEHAELDNNDLTPGRLFQLGYLKKVLRCPAGGQYAITQ
                     EKGYIEASCFKTLDGTDHGSFK"
     gene            complement(2647..>4117)
                     /locus_tag="PKO06_07480"
     CDS             complement(2647..>4117)
                     /locus_tag="PKO06_07480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNV69518.1"
                     /translation="YLAYKTFPIHVALLRGTSADTEGNISMEKEALTLEVLSIATAAK
                     NSGGFVIVQVERIVERGTLNPKLVKIPGILVDCVVVGKPENHWQTFATPYNPGFSGEV
                     RVPMESIAPMEMSDRKIIARRGAFELSPNSVVNLGIGMPEGVAAVANEEKILEFLTLT
                     AEPGVVGGLPAGGLDFGAATNTDAILDQPYQFDLYDGGGLDVAFLGLAQTDASGNLNV
                     SKFGPKLAGAGGFINISQNAKKVVFVGTFTANGLEVEVKDGTLQIVKEGKTKKFLRMV
                     EHVTFSGAYAVQRGQPVLYVTERCVFQLTPKGMELIEIAPGVHLEKQILAQMEFEPII
                     NGTPRLMDARIFQPNAMGIREDLLGVPLQNRLVYDKHSGTFFVNFEGYAIRREEQVAE
                     IEQLVGRILSPLNQKVPTIVNYDSFSIVPELLDGYMAMVERLMKKYYTQVTRYTTSAF
                     LRMKLRTLLGLRGVAPHIYESREEAASVLEQLKSAAPKK"
BASE COUNT          785 a         1127 c         1205 g         1000 t
ORIGIN      
        1 ccatgccgcg cgaccaggtg tacgccagaa tggcgagcgt tcagcgcgaa caatatctgc
       61 agttcaccgt gaagcatcgt ctggcaaatg gagaagttcg ccccgtcgaa gtttcctgca
      121 gcctgcagtt ttttcagggg cgtgagttgc tgcattcgat cattcacgat atcagcgagc
      181 gttgtcgcgt cgagcaagag cgagaacacc tgatacaaga actccagtgt gctgtcgcca
      241 agatcaagac cttgcgcgga atgcttccga tctgcgccgc ctgcaagaaa gtgagagatg
      301 ataaaggcta ctggaatcaa atcgaagcct ttctgtcaga gttctcagaa ctcgagttca
      361 gtcatggcat ttgtcctgac tgcaagaaac ggttgtatcc cgatctcgag gactgagccg
      421 gctggtaccc tcgacttttt ttctctgagc gcgtgattgg tcgatcgccc gattctcttt
      481 ttctgacggg tgtggtatca ctgagacccc ggcgtgtgat gcggagtctc atttcattct
      541 gtggagccca ggcatggatc tgctgtcggt tgatcgactg aaaaaggcct atcagtcacg
      601 tggtcgtgaa ccattctggg cggtgaaagg catctccttt acgcttgggc cgggggagtg
      661 ctttgggttg ctcgggccga acggcgccgg aaagtcgacg tcgatgaact gcatcaccgg
      721 cttctatcag gcgagtgaag gagcggtgcg cctgatggga ttcgatgtgt atgcggaacc
      781 tcgccaggca cgtcagtatc tcggtgtctg tcagcaggaa gacaccctgg actcggattt
      841 caacgccttc gatcagatgg ttcgatatgc ctcgtttttc gagattcccg aggcagaagc
      901 ccgcacgaga acacagactc tgctgaatcg attcggactc tcggataagg cgcgtgagat
      961 ggttgaggag ctttcgggcg ggatgcgccg ccgtctgcag gtggcgcgag ccctggtgtc
     1021 gcagccgcga atcctggttc tcgacgagcc gacggcaggg ctcgaccctg aggcacggcg
     1081 gcagctctgg gatatcatca ccgagtatcg cgcgcaggga aactcgattc tgctcagtac
     1141 gcattacatg gaagaggctg agcgcttgtg tgatcgcgtc gcgatcatgc atcagggact
     1201 gattcttgat gtggcaccag cgtccgagtt gatcgcaaag tatatcggca cccagagcgt
     1261 tgaggaagag attcgaccgg gtcttctctg gaaacgattg cccaatcttg aagatgtgtt
     1321 cctgaagctg accggcagtc acctgcagga ggactcggaa gaatgacctc agccaaagcc
     1381 atacgtcttg tctggtggcg ttatttcgat gtctttcgca agaatgtcgg gtacagtgtc
     1441 gtgacaacgt tcgtcgaacc gttgttgtat ctggcctcgt tcggatttgg tgtcggcgcg
     1501 ttggtgccgg cgctgcagtt ggacggtatc agcgtgacct atcgccagtt cgttctggcc
     1561 ggcattgtgg cgcagacggt tctctttcaa gccttcttcg aggccgcgta tgggggcttc
     1621 gtgcgcatgt attaccagcg catctttcag gctatcgcgg tgaccccgat cacgttgtcc
     1681 gaagtgctct ggggggaact gctctggaac acctgtaagt cgggctttgc cgctgcggtc
     1741 gttgttctga ttggttgtac gatcggcgac tttcactggc gcggcgcgct gttcattctg
     1801 ccggtgatca ttctcggcgc gctcgttttt gcctccatgg ggcttctggt tgccgctcgc
     1861 tcccgatcga tcgataacat ttcttttccc cagttcctgc tggtgtttcc gatgtttctg
     1921 ttctgtgggg tgttctttcc gcttgaacag ttgccgtcga ccatccagag cctcgtgtgg
     1981 gtgttgccgc tgacgccgct ggtgtcgctc attcgctcgc tgatgctggg gttgccgctg
     2041 ttctggccgg cctggctgat actgattctc tgggccgccg tgttggttcc catcgcccgt
     2101 cgtgccatgc gcgcccgcct ggtgaaatga accgggaaac gacctgaggc tctccgcatt
     2161 ggatccgata ttccggtcaa ggttgaccgc cggatgtcag ctgatgtacg tacaacgcat
     2221 gatgttcacg gcacggcgcg gttttaccct gatcgagatc atgatcgtcg ttctgattat
     2281 tggcattctc gtcacgttcg cgtcattcag ctatcgacac ctgcgccagc gggtgcagcg
     2341 ggtttcctgc cgtgagaacc tgcgtattct gcagcaagcc gccgtgttat gccagaccga
     2401 acacgcggag ctcgacaata atgatctgac cccagggcgt ctctttcagc tgggatattt
     2461 gaagaaggtt ctgcggtgtc cggctggggg gcagtatgca atcacccagg agaaaggcta
     2521 catcgaagct tcgtgcttca agaccctgga cggaaccgat cacggttcgt tcaagtaagc
     2581 gacagcggaa aagagacacc ggccacgctt tcccttgcag gagtcgtggc cggtgctgtc
     2641 tatcagttac ttcttgggtg cagcgctctt cagctgttcg agtacgctgg cggcttcttc
     2701 gcgactttcg taaatatggg gggctacacc acgcaatcca agcagcgttc gcagtttcat
     2761 gcgcaggaac gcgctcgtcg tgtaccgggt gacttgcgtg taatacttct tcatcagcct
     2821 ctcgaccatg gccatgtatc catcgagcag ttccgggacg atcgaaaagc tgtcgtaatt
     2881 gacgatggtt ggcaccttct gattgagcgg actcagaatg cgtccgacca gttgttcgat
     2941 ctcggcgacc tgttcctcgc gtcgaatggc gtacccctcg aaattgacaa agaatgtgcc
     3001 gctgtgcttg tcatagacca atcgattctg caacggcacg cccagcaggt cttccctgat
     3061 gcccatggcg ttcggttgaa agatgcgtgc atccatgagt cgcggagtgc cgttgatgat
     3121 cggctcgaac tccatctgtg ccagaatctg cttctcgagg tgaacgccgg gggcaatctc
     3181 gatcagttcc attcctttcg gagtcagctg gaacacacag cgctccgtca cgtagagtac
     3241 tggttgtcca cgctgaaccg cataggctcc actgaaggtg acatgctcga ccatgcgcag
     3301 aaacttcttg gtcttccctt ccttgacgat ctgcagtgtg ccgtctttca cctcgacttc
     3361 gagtccattg gccgtgaagg ttccgacaaa gacaactttc ttcgcgttct ggctgatatt
     3421 gatgaagccg ccagctccgg cgagtttcgg accgaacttg ctgacattga ggttgccgga
     3481 agcgtcggtt tgcgcgaggc cgagaaacgc gacgtcgagc ccgccgccat cgtacagatc
     3541 gaactgatac ggttggtcaa gaatcgcatc cgtgttggtg gccgcgccga aatcgagccc
     3601 gccggccggc aggccgccga ccacgcctgg ttcggcggtc agggtgagaa actccagaat
     3661 cttttcttcg ttggcaaccg cggccacacc ctcaggcatg ccgattccca gattcaccac
     3721 gctgttgggg gagagttcga acgcgccgcg gcgggcaatg atcttgcgat cgctcatttc
     3781 catgggggca atcgattcca tgggcacgcg cacttcgccg ctgaaccccg gattataggg
     3841 cgtggcaaac gtctgccaat gattctcagg tttgccgacg acgacgcaat cgacgagaat
     3901 tccgggaatc ttcaccagct tggggttgag tgttccgcgc tcgacaattc tttcaacttg
     3961 tacaataacg aagccgccgg aattcttggc ggccgtggcg atcgacaaaa cctcgagcgt
     4021 cagggcttcc ttttccatcg agatgttgcc ctcggtatca gccgatgtcc cacggagaag
     4081 cgcaacatgt atcggaaacg tcttgtacgc gagatac
//
LOCUS       DAORHD010000292         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23604_length_4098_cov_4.221865, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000292 DAORHD010000000
VERSION     DAORHD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23604_length_4098_cov_4.221865"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKO06_07485"
     CDS             <1..522
                     /locus_tag="PKO06_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV69519.1"
                     /translation="GEVIDTHTADGLKVAREHDLIVDQLGELERVLVRSGPAAALPLM
                     SQALPGFAEAMRQHFFFEERIFYPALATTIPTAGIIERILLLQKEHGLMEERLRLLTQ
                     LIQAPCPSAEAFVALVHHELLDLVNLVKRHAVVEITDIFPRINQNPLCLKMVLDLAQR
                     ETNKAEKKATPEA"
     gene            complement(563..1807)
                     /locus_tag="PKO06_07490"
     CDS             complement(563..1807)
                     /locus_tag="PKO06_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV69520.1"
                     /translation="MKKFFDCIRVPEEKSLLSFFEDTFNLRTVNDLISFKENEAPDNY
                     LPFYMRGYYLPINDSFGGEINKICQHARQKLGFKKNEIEFYVANDAEFNSSSILSTSK
                     NRPHIVIINRGLLDRVNIDELAFVMGHEIGHLIYQHSYASRVIQFVYPEYENMPPFLQ
                     KLYDVWSKLGEISADRIGLLVCQKIETAVRALFKLSSGLDDRSFQVSFDNLVKISEDT
                     FYEMAQNPSYVSASHPANPIRIKALTEFYNSKMGKSIMKGKICAADAPLEEKINEILM
                     LIRKIPFNEEEELSLALLASAGMKIMLSEPRPSDEEYDYLLNVLSDFIHWPPAYLDQL
                     NPDNLEEVLKTSSKTLVEKYPQRVRDLLRNLFPIIVRDKKLKNGEVTTFMSIATDDLK
                     IPVSEVVELFLDGLRELFKPGY"
     gene            complement(1890..2228)
                     /locus_tag="PKO06_07495"
     CDS             complement(1890..2228)
                     /locus_tag="PKO06_07495"
                     /inference="COORDINATES: protein motif:HMM:NF013920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV69521.1"
                     /translation="METSILGFKCKSCGHVHYPNRARCRKCHNPEFEIVPLPKTGTLL
                     TYTHAHALPGDFEVPKLTLGIVLLENGNRITGQLDIPNPRIGMKVQGRIDTVRKTGYQ
                     TFKGMIFREI"
     gene            complement(2230..3405)
                     /locus_tag="PKO06_07500"
     CDS             complement(2230..3405)
                     /locus_tag="PKO06_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HNV69522.1"
                     /translation="MRDVFAIGLGITSFTRLEYPLIEIAAYSSLMALKDCGLDRVDHV
                     YVANMGGGRVNKQTALGSALVDHLSLCPAGAETIENGPASGASAIKTAYMAIASGLHD
                     IVMVTGCERMNEVNSLDATDFVASLAHPLAEQIYGVTLPSLAGMFTRLYMEKYGVTSR
                     HLAMVAVKNHDHALLNPFAHVRQAITLEGILDDKDAATNNPIIADPLRFFDLCPVSDG
                     AATVILATADVAKKCGRPMVRLAGVGQATDTHAVHERDEPTDLLAVRGAAEKALGMAG
                     LKPSEVNVAELHDAFTILEIVESEEVGFFKKGEGHLALAEGYTRLGGKLPINPSGGLK
                     AKGHPVGATGVGQAHEIITQLRGEARKRQVSGARIGLTTNFGGFGNNVVSLVFTKEN"
     gene            complement(3398..>4098)
                     /locus_tag="PKO06_07505"
     CDS             complement(3398..>4098)
                     /locus_tag="PKO06_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69523.1"
                     /translation="KCMTLANGEKMTIRQIGREEIPQLLPHVEALMHIERDFYDIVAS
                     RVYSELLGYYRYRVVDEYVLVAQVDGELAAIVNGRFVNKDIGMSYHTLAFRRGLRVGA
                     HAFASKMEYHMDIVGQKEVMVVAESPIGFRRWMIEYNLAPRFEYPHELGGCPSYALTR
                     ELYEKAKDTLIVGSRPVPEALLKKAKAAILPPSNPPKMPPDLLAATRAKYDSTGTALM
                     ASRWALGKEDQHDA"
BASE COUNT          844 a         1133 c         1208 g          913 t
ORIGIN      
        1 ggcgaggtca tcgataccca taccgcggac ggcctgaagg ttgcgcggga gcatgatctg
       61 atcgtcgatc agttgggcga gcttgaacgg gtgctggtgc gcagcgggcc cgctgccgct
      121 ttgccgctga tgagtcaggc gctgcccggg ttcgccgaag cgatgcggca acacttcttc
      181 ttcgaagaac gaatcttcta tcccgcgctc gcgacaacca tcccgacggc agggatcatc
      241 gagcgcatac tgctgttaca gaaagagcac ggtctcatgg aagagcgcct gcgcttgttg
      301 actcagttga ttcaagcgcc ttgcccctcc gctgaggctt ttgtggcgct cgttcatcat
      361 gaacttctcg atctcgtgaa tctggtgaaa cgtcatgccg tcgtcgagat taccgatatt
      421 tttccgcgca tcaatcagaa tccgttgtgt ctgaagatgg tcctcgatct ggcccagcga
      481 gaaacgaaca aggccgagaa aaaagcaacc ccggaagcct gaatggccgc cggggttgtg
      541 gggttgtgtc cgagtctgtt ggttagtatc cgggcttgaa cagctcgcgc agaccatcaa
      601 gaaacaattc aacgacttcg gaaaccggga tcttcagatc gtcggtcgca atcgacatga
      661 aggtcgtcac ttcgccgttc ttcagtttct tgtcgcgaac gatgatcgga aacagattgc
      721 gcagcagatc acgcacgcgc tgcggatact tttcgaccag cgttttcgaa gaggtcttga
      781 ggacttcctc cagattgtcc gggttcaact gatcaagata ggccggcggc cagtggatga
      841 aatcggaaag cacgttcagc aggtaatcgt actcttcatc actgggccgt ggctcggaga
      901 gcatgatttt catgccggcc gatgccagca gagccagaga cagttcttct tcctcattga
      961 acgggatctt gcggatcagc atcagaatct cgttgatctt ttcttcgagt ggcgcgtctg
     1021 ccgcgcagat cttgcctttc atgatgctct tgcccatctt cgagttgtag aactctgtga
     1081 gcgccttgat gcgaatcggg ttggccgggt gtgatgccga cacgtagctc ggattctgcg
     1141 ccatttcgta gaacgtgtct tccgagatct tgaccaggtt gtcgaaactg acctggaaac
     1201 tgcgatcgtc caggccggaa ctgagtttga acagcgcccg aaccgcggtt tcaatcttct
     1261 gacacacgag caatccgatg cggtcggcag agatttcgcc gagtttgctc cagacgtcgt
     1321 acaacttctg caaaaagggc ggcatgtttt catactcggg ataaacgaac tgaatgacgc
     1381 gcgaagcgta cgagtgctga tagatcagat gcccgatctc atgccccatg acgaaggcta
     1441 gttcgtcaat gttgacgcga tccagcaggc cgcggttgat gatgacgata tgcggacggt
     1501 tcttcgaggt cgagagaatc gaggacgagt tgaactcggc gtcgttggcc acataaaact
     1561 cgatctcgtt cttcttgaac cccagtttct gccgggcgtg ctgacaaatc ttgttgatct
     1621 cgccgccgaa cgagtcgttg atcggcagat agtacccgcg catgtagaag ggcaggtaat
     1681 tgtccggggc ttcattctcc ttgaaactga tcaaatcatt gactgtgcgc aggttgaatg
     1741 tatcttcgaa gaagctgagc aggctctttt cttccgggac acggatgcag tcgaagaact
     1801 ttttcatgag tgtttccttg tgtgtgattc gcgcggcata cctgttcggt atgccgcgcg
     1861 cgaagcgtga ccgtgcagtg acaccgcgat cagatctcgc ggaagatcat gcccttgaag
     1921 gtctgatacc cggttttgcg aaccgtgtcg atgcgaccct gcactttcat gccgatacgc
     1981 gggttcggaa tgtcgagctg gccggtaatg cgattcccgt tttcgagcag gacgatgccc
     2041 agtgtcagct tgggaacctc aaagtcgccc ggcagggcat gggcgtgggt gtaggtcagc
     2101 agcgtgccgg ttttgggcag cggaacgatt tcgaactccg ggttatggca cttgcggcat
     2161 ctcgcccgat tcgggtagtg aacgtgacca cagctcttgc acttgaagcc aagaatcgaa
     2221 gtttccatgt cagttctcct tcgtgaagac gaggctgacg acattgttgc caaagccgcc
     2281 gaagttggtg gtgagtccga tgcgggctcc cgagacctgc cgcttgcggg cttcgccgcg
     2341 caactgagtg atgatctcgt gcgcctggcc aacgccggtg gcaccgaccg gatgtccctt
     2401 ggccttcaac ccgccggaag gattgatcgg cagcttgccg ccgagtcgcg tgtatccttc
     2461 tgccagagcc aggtgtcctt cacctttctt gaagaatccg acttcttcgc tctcaacgat
     2521 ctcgagaatc gtaaacgcat cgtgcagctc ggcaacgttg acttcggaag gcttgagccc
     2581 ggccatgcca agcgctttct cggcggctcc gcgcaccgcc agcaagtcgg tcggctcatc
     2641 acgctcgtga acggcatgcg tatccgtggc ctgaccaacg ccggccaaac gtaccatcgg
     2701 tcgaccgcac ttcttcgcga cgtcggcggt ggccagaatg accgtcgcgg ctccgtctga
     2761 aacgggacac agatcgaaga agcgcaaagg atcggcgatg atcgggttgt tggtcgcggc
     2821 atccttgtcg tcgagaatgc cttccagggt gatggcctgg cgcacgtggg cgaacgggtt
     2881 cagcagggca tggtcatggt tcttcacggc aaccatggcc aggtggcgcg atgtcacgcc
     2941 gtacttttcc atgtacaagc gcgtaaacat gccggccagc gatggaagcg taactccgta
     3001 gatctgttcg gcaagagggt gcgccaatga cgcgacgaaa tcggtggcgt ccaggctgtt
     3061 gacttcgttc atgcgctcgc agccagtgac catgacgatg tcatgtaacc ccgaggcgat
     3121 agccatatac gcggttttta tcgccgaagc gcccgaggcg ggaccatttt cgatcgtctc
     3181 ggcacccgcg gggcacaggc tcaaatggtc gaccagagcg ctgccgagag ccgtctgctt
     3241 gttcacgcga ccgccgccca tattggcgac gtagacatga tcgacgcggt ccagcccgca
     3301 gtctttgagc gccatgaggg aagagtaggc cgcaatctcg atgagcgggt attcgagacg
     3361 ggtgaacgag gtgattccaa ggccgatggc aaagacgtca cgcatcgtgc tgatcctcct
     3421 ttccgagagc ccagcgactg gccatcaggg cggtgccggt cgagtcgtat ttggcgcggg
     3481 tggctgccaa cagatcgggc ggcatcttgg gcggattcga cggcggcaga atggcggctt
     3541 tcgccttttt cagaagcgct tccggaaccg gtctcgaccc gacgatcaga gtatccttgg
     3601 ctttctcata cagttcccgg gtcagcgcgt aactgggaca accgccgagt tcgtgcggat
     3661 actcgaagcg cggcgccaga ttgtattcga tcatccagcg cctgaatccg atgggcgact
     3721 cggcaacgac catgacttcc ttctggccga cgatatccat gtggtattcc atcttcgagg
     3781 cgaaggcatg agcacccaca cgcaatccgc gtctgaaagc cagcgtgtga tacgacatcc
     3841 cgatatcctt attgacgaac cggccgttga caatggcagc aagctcacca tcgacctgag
     3901 caaccaggac atattcgtca accacgcgat accggtagta gccgagaagt tcggaataca
     3961 cgcgcgaggc gacgatatcg tagaaatcgc gttcgatgtg catcagggct tcaacgtgcg
     4021 gcagcaactg gggaatctct tcgcggccga tctggcgaat cgtcatcttt tcgccattgg
     4081 cgagcgtcat gcacttgg
//
LOCUS       DAORHD010000293         4097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23610_length_4097_cov_3.503464, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000293 DAORHD010000000
VERSION     DAORHD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23610_length_4097_cov_3.503464"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1804)
                     /locus_tag="PKO06_07510"
     CDS             complement(<1..1804)
                     /locus_tag="PKO06_07510"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV69524.1"
                     /translation="MSDEMGPTPLGKLVIFLFIVGCILFAALNLSPAFKAQVYQALGW
                     ATPVQPNPANPGDKGTTSPDPANQVSQGSSGSTGTTNTPSSSSGAVEAQDPKGITTVS
                     EYQFIPQQRLPPVKGVSSYEWDEKNKVVVFPINVWIGWLPIVAANHGFKPNEESVFFK
                     KYGFKVDLKLIDDPLVARDTFASGKSHILWGTLDMMALFAGELMKDSRTSPRIYQQID
                     WSNGGDGIVVRGNIRSVKDLRGKTVALAQNSPSEYFINNLLINAGLSPNKITPKYTAT
                     AFEAAAAFVSDKSIDACVSWAPDIYSIPEKVTGTRILTTTADANRLIADVWAARADFA
                     KDHPEIIKGLVAGIFEGMSLIKNSAFKAKAFQWMADGYNLKFDDVKSMENDAHITNYP
                     ENKEFFLNANNPTNFERTWSNINMVYRDLGKVATPIRFDEVMDFSVLQALEKDSGFKD
                     AVKDQTEVTVSRFAPTSYKKVSAEAPVITQTVRINFYPNSANVFEPEHDELGNGLANT
                     LYDPNVEATLEKIARMVGQYERATVAIVGHTDSSMKGKVPEKAVSDLSKDRASAVRDA
                     LIRRYKFPLNKFVTEGKGWNDPANPDEPNNHTLNR"
     gene            complement(1927..>4097)
                     /locus_tag="PKO06_07515"
     CDS             complement(1927..>4097)
                     /locus_tag="PKO06_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69525.1"
                     /translation="SRNPLAPGYLFQHLAPSNTCHFNIIIAKSFSQGSHTTERLGRFS
                     FMGLLFWYAAGILLLGHLFRLTGYLRLSLTTILVWSCLLILAPPLLQSLVNFEGGDVE
                     REARLERDARTTLGRVLQSESEAFLQIAGFTGNLLRTHVRQPPLVRGIDSPAPPSSDD
                     FTSSATEHLKARLLSLLPRGGLVQYAYIFGPNGPIASYMDDPTRARNLDRFQKLGSFY
                     GQLYREFLRRLDPNSARRQSAKEQRESMGFWLQFEEVLTLITWVFPTESFLEMILSPG
                     TFVQADFFVNQSTFAHIPLYDRGQPRLVFHSSCQRDALSPNFLREFSEARHRFEQVDI
                     RIWPAWYDSSYLALRPPYQIIELGRILSGKHEFPYPQWPEMGRLAGAAFSQRGPVFGE
                     RLQGHERQLLGAIKSDNCESFAFCAALPLGTVLDESASRQRMVRWVLAGVVFSALLLA
                     WMVSRLVLRPIRDLTGAVRQVTGGNFSVLLPTQSRDEVGQLNQAFNQMVRGVRERQRL
                     ARFLSDSVRTAVQEEDVQAGIASASTKVVTVLFAGISGLRDKRHRLSPQQLMLELNQL
                     IERLTATVIAAQGVVDKIMGDKLLAVFYPAPHESPAQAVARALQAAERLLQAAHSLPA
                     WQQERLGIGLVTGPVISGILGTPEIRLEMTVIGDTVNLASRLCDVALQLPDGGVVSEP
                     ETRQALERVFVDDRQREFRRLAVSKVKGKSREVEVFQIRV"
BASE COUNT          844 a         1126 c         1258 g          869 t
ORIGIN      
        1 gccgattcag cgtgtgattg ttcggttcat ccggattggc tgggtcgttc cagcccttgc
       61 cttccgtgac gaacttgttg agagggaatt tgtaccgacg gatcaacgcg tcgcgaacgg
      121 cgctggcgcg gtctttcgaa agatcactga ccgccttctc gggaaccttg cccttcatcg
      181 agctgtctgt gtgaccgacg atggccacgg tggcgcgctc atactgaccg accatacggg
      241 cgatcttttc gagagttgct tcgacgttgg gatcgtacaa ggtgttcgcc agtccgttcc
      301 ctagttcatc gtgctcgggc tcgaagacat tcgccgaatt ggggtaaaag ttgatgcgca
      361 ccgtctgcgt gatgaccggg gcttcggccg aaaccttctt gtatgaagtc ggcgcgaatc
      421 gggaaacggt gacttctgtc tgatccttta ccgcatcctt gaacccggaa tccttttcga
      481 gtgcctgcag caccgagaaa tccataactt cgtcaaaacg aatcggagtc gcaaccttgc
      541 ccaggtcgcg gtacaccatg ttgatgttgc tccaggtgcg ttcgaagttg gtcggattgt
      601 tcgcattcag gaagaactct ttgttttcgg gatagttcgt gatatgcgca tcgttttcca
      661 tgcttttcac atcgtcgaac ttgagattgt acccatccgc catccattga aacgccttgg
      721 ccttgaaggc cgaattcttg atcaggctca tgccttcgaa gataccggct accagcccct
      781 tgatgatctc ggggtggtct ttggcaaagt cggctcgtgc cgcccagacg tcggcgatca
      841 ggcggttggc atcggcagtc gttgtcagaa tgcgggttcc cgtgactttt tcgggaatgc
      901 tgtaaatgtc cggagcccag ctgacgcagg cgtcgatgct cttgtcactg acaaaggccg
      961 ccgcggcttc gaatgcggtt gcggtgtact ttggggtgat cttgttgggc gataacccgg
     1021 cattgatcaa cagattgttg atgaaatact cggacggact gttctgggcc agggccacgg
     1081 ttttgccgcg caggtctttc acggaacgga tgttgccgcg aaccacgatg ccgtcgccgc
     1141 cgttcgacca gtcgatctgc tggtagatgc gcggtgacgt gcggctgtct ttcatgagtt
     1201 cgccggcaaa caaggccatc atgtcgagcg tgccccagag aatgtgcgat ttcccggagg
     1261 cgaaggtgtc gcgggcgacc agcgggtcgt cgatcagctt gagatcgacc ttgaagccat
     1321 acttcttgaa gaagacgctc tcttcgttgg gcttgaaccc gtggttggcg gcaacaatgg
     1381 gtaaccagcc gatccagacg ttgatcggaa agacgaccac tttgttcttt tcatcccatt
     1441 cgtagctcga gacgcctttg accggcggca ggcgctgctg tggtatgaac tgatactcgc
     1501 tgaccgtcgt gatgcccttt gggtcctggg cttcgacagc acccgacgat gatgacggtg
     1561 tgttcgtcgt gccggttgac ccgctcgatc cctgcgacac ctggtttgcc gggtcggggc
     1621 tcgtggtgcc cttgtcgccg gggtttgccg ggttcggttg aaccggagtc gcccagccca
     1681 gggcctgata cacctgtgcc ttgaaggctg gagaaaggtt cagagcggcg aatagaatac
     1741 agccgacgat gaacagaaag atgacgagtt ttcccagggg ggttgggccc atttcgtcgc
     1801 tcatcagtgg agtcctcctc gtgattgctg tccgtcaggg gcggagacat cgttgttcat
     1861 gccgaccaca tcggctggag acaaaccgtg agatataata acgggaaccg tctgggaagg
     1921 caaccgtcag acgcgaatct ggaaaacctc gacttcgcgg ctttttccct tgaccttcga
     1981 gactgccagt cgccgaaact ctctctggcg gtcgtccacg aatacccgtt ccagggcctg
     2041 gcgggtttcc ggttccgaga ctacgccgcc atcggggagc tgcaaggcga cgtcgcacag
     2101 acgggaagcg agattgaccg tgtcgccaat gacggtcatt tccagccgga tctcgggagt
     2161 tcccaggatg ccggaaatca ccggcccggt gaccaggccg attcccagcc gttcctgctg
     2221 ccatgcggga agggagtgag cagcctgcag cagtcgttcg gccgcttgca gagcgcgtgc
     2281 caccgcctgg gccggagact cgtgcggcgc cggataaaaa acggcgagca acttgtcgcc
     2341 catgatttta tccacgaccc cctgagcggc gatgacggtc gcggtcaacc gctcgatcag
     2401 ctggttcagt tccagcatca gttgctgtgg agacagtcgg tgtcgcttgt ccctgagccc
     2461 ggagataccg gcgaagagga cggtcactac tttggtggag gcggatgcga tgccggcctg
     2521 cacgtcttcc tcctgaacgg ccgtacgcac ggagtcggag agaaaccggg ccagtctctg
     2581 ccgttctcgc accccgcgca ccatttgatt gaatgcctga ttcaactgtc caacttcgtc
     2641 acgcgactgg gttggcagca ggacggagaa gttgccgccg gtgacctgac gcacggctcc
     2701 ggtgagatct cgaatcggtc gcagaaccag acggctgacc atccatgcca gcagcaaggc
     2761 cgaaaacacc acgcctgcca gcacccagcg aaccattcgc tggcgcgagg cagactcatc
     2821 aagaacggtc ccaaggggga gcgcggcgca gaaggcaaaa ctctcacagt tgtcactctt
     2881 gatggctccg agaagctggc gctcatggcc ctgaagacgc tcgccaaaca ccggtccgcg
     2941 ttgcgaaaaa gccgctcccg ccagacgacc catttcgggc cactgcggat acggaaactc
     3001 atgcttcccc gacagtattc gtccgagttc gatgatctga tacggtggtc gcagagcgag
     3061 ataagagctg tcataccagg ccggccagat acggatgtcg acttgctcga atcggtgacg
     3121 agcttcagaa aactctcgca ggaagttcgg cgacagcgca tctcgttgac aactcgagtg
     3181 aaacaccagt cgaggctgac cgcgatcata gagaggaatg tgggcgaacg tggattgatt
     3241 gacgaaaaag tccgcttgta caaatgtgcc gggcgacaga atcatttcga gaaatgactc
     3301 cgttggaaag acccaggtga tcagagtgag cacttcttca aactgcaacc agaagcccat
     3361 cgactcgcgt tgttccttcg cggactgccg ccgggcggag ttcggatcga gacggcgcag
     3421 aaactcgcga tacagctggc cgtagaagga tccgagcttc tgaaagcggt cgaggtttcg
     3481 tgcccgggtt ggatcatcca tataggaggc gatgggcccg ttcggtccga agatataggc
     3541 atactgaacc aaacctccgc ggggcaacag actcagcagg cgggccttga gatgttcagt
     3601 ggccgaggag gtgaagtcgt cggatgacgg gggggccggg gaatcgattc cacggacgag
     3661 tgggggctgt ctgacatggg tgcgaaggag attccccgtg aacccggcga tctgaagaaa
     3721 ggcttcgctt tcgctctgca gaacccttcc cagcgtggtt cgggcatctc gttccagtcg
     3781 agcctcacgc tcgacgtctc ccccctcgaa attgaccaga ctttgcagca gcgggggcgc
     3841 gaggatgagg aggcagctcc agacaagtat ggtggtcagg gaaaggcgca ggtatccggt
     3901 cagccggaag aggtggccga gcagcagaat ccctgccgca taccagaaga gcaggcccat
     3961 gaacgaaaaa cgtccgagtc tttcagtcgt gtgagagcct tgagaaaacg atttcgcaat
     4021 gatgatattg aagtgacagg tgttgctcgg tgcgagatgt tgaaaaaggt atccgggagc
     4081 gagagggttt cgcgatg
//
LOCUS       DAORHD010000294         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23785_length_4076_cov_3.241731, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000294 DAORHD010000000
VERSION     DAORHD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23785_length_4076_cov_3.241731"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..395
                     /locus_tag="PKO06_07520"
     CDS             147..395
                     /locus_tag="PKO06_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV69526.1"
                     /translation="MQDRVLTFLRKQLEKKAPLPPESTLPAYRYLDRGHVDSLGLIKF
                     IFEIEEEFQIQLTPEDTQSDEFRTIGGLAAMIRGKLPK"
     gene            392..2308
                     /locus_tag="PKO06_07525"
     CDS             392..2308
                     /locus_tag="PKO06_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2172 domain-containing protein"
                     /protein_id="HNV69527.1"
                     /translation="MTAPQASSYTRADILQALTAAGLRSGDTVFVTTSLGMLGVPEGV
                     ASGEDLNRLFFETLRECLGERGTIIVPTYSYTFGRSTATEPQIYDPLTTPSAIGPFPE
                     FFRRQPGVVRSLDPMMSVAGHGPATENLFRDLPKTSYGADSVFARLAHVSAKCCSIGL
                     GPNWMPFIHHTDWLRQVPFRYDKLFHGIIRTGQHEQPTAWVYSVAMLHQASHSTGHKV
                     AKRAVEAGVWQFAPLGRARIYVADYRRYFDFTLEQLETDPWITADGPPGDPLALERER
                     VPEERSIIAEPETHSIDAILLQHAPRHRTDVSDASHHLLHDLAKHEGLELYSFKSGEN
                     HFDWIIPERWNLQSVSLHDTQGHPVLAEDLLRARVYGHSLSVDRQLSKSELLPHLSMS
                     RENHSLPRYRHTITNRDWGFCLSRQEFEALPSMNLHAHIVSAYSYGLMTVACSPITTS
                     NRPLLLLTACVSGPSGGKDLLGVSTALQAARQLRHAASSRNWECGVLLHSGPAGFAAW
                     LDQRSDVRHRLAGIVEIRHPLSPDSLPIVVFRKSDQYGLNSHLQHLRPAAEISESDSQ
                     RPYLLTRGQNPVAQKSIPENEFPVISIGAPLACEMSAGEYPFPYASLESADFALATAC
                     GHTLAGILQGFTTR"
     gene            2352..3707
                     /locus_tag="PKO06_07530"
     CDS             2352..3707
                     /locus_tag="PKO06_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNV69528.1"
                     /translation="MNSTSEIRWDPIESILAGASEEKLLERGDELYRWLSDLFPVCRS
                     LTGNGVRQTISYFQRLLPGLQIHEVPSGTAAFDWTVPAEWNIRAAWIKDSQGKTVVDF
                     AHNNLHVVGYSIPVQATLSLDELQPHLHTLPEQPDAIPYITSYYKPYWGFCLTERQRA
                     TLGPGEYTVCIDSTLEPGSLTYADLVIPGKSAREVFLSTYVCHPSLANNELSGPVIMA
                     AIGQYLLAHAPHLEYTYRLVFIPETIGSIVYLSRHLEHLQKQVVAGFNLTCLGDDKSY
                     SVLHSRYGNTYADRIASHIVRTLCPDAKHYSYLTRGSDERQYCAPGIDLPFVSLMRSK
                     YAEYDAYHTSLDNLDFVSPAGLQGGFRFVRQCLNLIEHNFRYRTTVLGEPQLGKRGLY
                     PSLSTKHSYRQVSLLKNVIAYSDGTQDILDLAERFECHANDIIRIYHILLDHHLVTRV
                     D"
     gene            complement(3746..>4076)
                     /locus_tag="PKO06_07535"
     CDS             complement(3746..>4076)
                     /locus_tag="PKO06_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV69529.1"
                     /translation="SGSAEWYQVNPEELILSGRTGKREIVDRASGATIAAAPRYTRFK
                     AGHPAGFLEAFANLYVDIADCVSQYKRQGIWQSDEVFGAELALDGLRFMEAMVRSQTT
                     GRWEPVG"
BASE COUNT          848 a         1289 c         1041 g          898 t
ORIGIN      
        1 tcacaaagcg gagcatcttt gggaaatacc ccgttatgtt gactcgctaa gtccccggta
       61 tgagttcaga atgcgattgc acggcaactt tggtcgcgaa ctgactctct actgcatcat
      121 gcccgagtaa tcctctggag gaacctatgc aggaccgagt tctgaccttt ctgcgcaaac
      181 agctggaaaa gaaagctcca cttccgcccg agtccaccct tcccgcttac cggtacctgg
      241 accgcggcca tgtcgactca ctcggactca tcaagttcat cttcgagatt gaagaggagt
      301 ttcagattca gctgacaccc gaagacaccc agtccgacga gtttcgcacc attggcggtc
      361 tcgccgccat gattcgcggc aagctgccga aatgaccgcg cctcaggcct cgagttacac
      421 gcgcgccgac attctgcagg ctttgacggc ggccgggctt cgatccggtg acaccgtgtt
      481 cgtcaccacc agcctcggca tgctgggagt tcccgaaggg gtcgcttcag gcgaagacct
      541 gaatcgcctg ttttttgaga ctctgagaga atgcctggga gaacgcggca ccatcatcgt
      601 tccgacgtat tcttacactt tcggtcgcag caccgccacc gagccgcaga tctatgatcc
      661 gctgaccacc ccctccgcca tcggtccgtt ccccgaattt tttcgccggc aacctggcgt
      721 cgttcgctct ctcgatccga tgatgtctgt ggccggtcat ggaccggcca cagagaattt
      781 gtttcgagat ctccccaaaa catcatacgg agcggattcc gtcttcgccc gacttgcaca
      841 tgtctcggca aagtgttgca gcataggcct tggtccgaac tggatgccgt tcattcatca
      901 cacggactgg ctgcgccagg tgccattccg atacgacaaa ctctttcacg gcatcatccg
      961 caccgggcaa cacgagcaac caaccgcctg ggtctattcc gtagccatgc ttcatcaagc
     1021 ttcgcactcg accggtcaca aagtggcaaa acgcgccgtc gaggccggag tctggcagtt
     1081 tgcccctctg ggcagagctc gcatctatgt ggcggactat cggcgctatt tcgattttac
     1141 cctcgagcaa ctcgagaccg atccctggat caccgccgac ggtcctcccg gcgatccgct
     1201 ggcgcttgaa cgcgagagag taccagaaga acgatcgata attgcagaac cagagacaca
     1261 ttccattgac gccattctgt tacagcatgc tccccggcat cgaacggatg tctcggatgc
     1321 ctcacaccac ctgttacatg atctggcaaa gcacgaggga ctggagttgt actcgttcaa
     1381 gtcgggagaa aaccacttcg actggatcat tcccgaacgc tggaatctcc agtctgtatc
     1441 cctgcacgat acgcagggcc atccggttct tgccgaagac ctgctgcgag cgcgcgtcta
     1501 cggacactcg ctcagtgtcg accgtcagtt gtccaagtcc gagctgctcc cgcatctcag
     1561 catgagccgc gaaaatcact cgttaccccg ctatcgacac acgatcacga atcgtgactg
     1621 gggattctgt ctctcgcgcc aggagttcga agcgttgccg tcgatgaacc tgcacgcgca
     1681 cattgtgtca gcctactcat acggtctgat gaccgtggcc tgctcgccca ttacgacaag
     1741 caaccgcccc ctcctgctcc tgaccgcctg cgtgagcggc ccaagcggcg gcaaggatct
     1801 gttgggtgtc agcaccgccc tgcaggcggc gcggcagttg cgccacgcgg catcttcacg
     1861 gaattgggag tgcggagtgc tgttgcactc cggtccggct ggatttgctg cctggctgga
     1921 tcaacgttct gatgtgcggc accgtcttgc cggcattgtc gagatacgtc atcctctgtc
     1981 gccggactct cttcccatcg ttgtttttcg caaatccgac cagtacggcc tgaactctca
     2041 cctgcagcac ttgagaccgg cagcggagat ctcagagtcg gacagccagc gaccgtatct
     2101 actgacccgg ggccagaacc ctgtcgccca gaagtccatt cccgagaatg agttccctgt
     2161 catctcgatc ggagctccgt tggcttgtga aatgtccgca ggcgagtatc catttccgta
     2221 tgcaagcctc gagtctgccg actttgccct ggcgacggcc tgcgggcaca cgctggcggg
     2281 catcctgcag ggcttcacca cgagatgact ccgggtcttt tcacgcctga gaaaggaaga
     2341 acagaagaga gatgaattca acctctgaga ttcgctggga tccgatagaa tccattctgg
     2401 ctggggcaag cgaggagaaa ctgctcgaac gcggtgacga gctctatcgc tggctatcgg
     2461 acctgtttcc tgtctgtcgc agcctgaccg gcaatggcgt ccggcagaca atctcttact
     2521 ttcagcgact gctccctggt ctgcagattc acgaagttcc ttccggaacc gcagcattcg
     2581 actggacagt accagctgaa tggaacattc gagcggcctg gatcaaggat tcgcagggta
     2641 agacggtggt cgactttgct cacaacaatc tccatgtcgt cggctattcg attccggttc
     2701 aggcgacgtt gtctctcgat gagctgcagc cacatcttca cacgcttccc gagcaaccgg
     2761 acgccatccc ctatatcact tcctactaca aaccgtattg ggggttctgc ctgacggaac
     2821 ggcagcgagc gacgctgggc cccggtgaat acacggtctg catcgacagt accctcgaac
     2881 ccggctcact cacgtatgcc gatctcgtga ttcccggaaa atccgcccgg gaagtgtttc
     2941 tctcaaccta cgtatgtcat ccgtcgctgg ccaacaatga actctctggc cccgtgatca
     3001 tggcggccat cggtcagtat ctgctcgcgc acgccccgca tctggaatac acctatcgcc
     3061 tggtgttcat tcccgagacg attggatcga tcgtctatct gagtcgacat ctcgaacatc
     3121 tgcagaagca ggtcgtcgcc ggattcaatt taacctgtct gggcgatgac aagtcgtatt
     3181 ccgttcttca cagccggtac ggaaacacct acgctgatcg catcgcatcg catatcgtcc
     3241 gcaccctgtg tccggacgcc aaacactact cgtacctcac gcgggggagt gatgaacggc
     3301 agtattgcgc tcccggaatc gacctgcctt ttgtctcact catgcgcagc aagtatgccg
     3361 agtatgacgc ctatcatacc tcactcgaca accttgattt cgtctctcct gctggtctgc
     3421 agggtggttt tcgcttcgtt cgccagtgcc tcaatctcat cgagcacaat ttccgatacc
     3481 gcaccaccgt tctcggagaa ccacaactcg gaaagcgtgg tctttaccca agtttgagca
     3541 caaagcactc gtaccgacag gtcagccttc tgaagaacgt gattgcctac tctgacggaa
     3601 cccaggatat tctcgatctc gccgagcgct ttgagtgcca tgcgaatgac atcattcgca
     3661 tctaccacat cctgcttgat caccatctcg tcaccagagt cgactgaaag ggtcacaaac
     3721 cattcacaga aagccgagca aggttctatc ccacgggctc ccagcgtccc gtcgtctgtg
     3781 atcgcaccat ggcttccatg aaacgcaatc cgtcgagcgc cagctccgct ccgaacacct
     3841 catcggattg ccagatcccc tggcgtttgt actggctcac gcaatcggca atgtcgacgt
     3901 agagattggc aaacgcctca agaaaccctg ccgggtgacc tgccttgaaa cgggtatagc
     3961 gcggcgctgc agcgatcgtg gctcctgaag cacgatcgac gatttcacgc ttcccggtcc
     4021 gaccgctcag gatcagttct tccgggttca cctgatacca ctccgcagac ccactc
//
LOCUS       DAORHD010000295         4054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23973_length_4054_cov_3.009002, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000295 DAORHD010000000
VERSION     DAORHD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23973_length_4054_cov_3.009002"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PKO06_07540"
     CDS             complement(<1..309)
                     /locus_tag="PKO06_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69530.1"
                     /translation="MKRIAILMCLVIMVVMATSPLRAAPANAEAAELVKQAEEAFKAQ
                     DLEKASTLMQQALTKEPDNAVFRYIFARILFAKKDYMSAKENFEIVSRSRPSKEKGDD
                     Y"
     gene            465..3440
                     /locus_tag="PKO06_07545"
     CDS             465..3440
                     /locus_tag="PKO06_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69531.1"
                     /translation="MKNKPLALIGAILFFAGLPVLLLNLGWEFLRREERTRVVSQHFE
                     ELERYLTRIKREGNTQEYIRNTLGRICGELNEEQLDATAAQRAVETLKTSGLSPIKLY
                     FFDAKGDGIAYPGQPREYQKIVQKLYLALSEPEAGGNDKLMQQYRSVFVSFLGTVEPT
                     ELMNQKFDLVRVVLNGKTAFFFWGTIYDSGFRGPHQDQSKTSDDPSYQGGVVAMVEEQ
                     HIDQNFALASLLQQLNRDRPAGYGFGCLNVRQIDRSFPKEDLEKWVRTPLPRLFALVQ
                     RLQSQFQRFHEYSDGLLTIAPYDDEKVLIGLQPQVVPLNPGLGRLIRLLSLFILGFVG
                     YWAWRIVYFERQVFVPIRRKLVVLFLYSTAIPMTALGLLGYQYVTDRREVLLQGIYKK
                     MAGFIGSLDDNFSVELDKLRSYYQRLARSKQVTLSDWEGFTELTSKLSDRNILQNVFI
                     GKADGKIVYMGAGRKGRGKNDMWAERLIPTFIRKIFSRHMPQVGFNPKNLMQDAMVDT
                     LADSLSDALAGGGGSGNAFQRIFEHSDVMHEMNLGNRINYFFTSFVRNPQGVIDRLML
                     ILIPKKRICTRYLRSIMARNKPRRQGADDFIRLGYIEDFGDRQVVPRELGKYGFVREL
                     FDKVVNTRTIQNSVQVMGGQTYLVVASPLKRIDEHVMLAFYPKQIVDEKVRNLTLGLL
                     VVATVSGLFAVFIGLALSRQFLAPIRELSTGITAIEKRDFRHQIPLLDNDELGSLGTT
                     FNQLMANLEELHVAKIVQETLFPQESLELGDYEIHGKTRSMSDLGGDYFDYFPVENRF
                     LVMLIGDVTGHGVPAALLMTMAKSGISIIGNDQATDVVRTLERLNQMMFETVKKKRMM
                     SFLYAVLDTATHQLHTANAGHNFPFLFRRRDRSACQIENTSYPLGARRKGVFKQLQFE
                     IQAGDALVMYTDGLIESQNPAGEPFSYPRLESTITRVGTEVSSAGMLHELISAEFDRY
                     ICGKTPEDDVTMIVMRRKG"
     gene            complement(3509..>4054)
                     /locus_tag="PKO06_07550"
     CDS             complement(3509..>4054)
                     /locus_tag="PKO06_07550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV69532.1"
                     /translation="NAKLYEQAITDGMTHLYLHRYFKQRLFDEIKRAIRFRRHLSLIM
                     VDIDHFKKFNDSYGHQMGDEVLKKVASILRKTIRTHDLPVRYGGEEFAVVLPETDIHG
                     AAAVAERIRRTIETEYLEHEGKVIKITASFGVSVFPECAQEMESFIKSADAALYHSKE
                     SGRNRVSEAPFLYPPPEPVEE"
BASE COUNT          903 a         1059 c         1137 g          955 t
ORIGIN      
        1 gtaatcgtcg cctttctctt tgctcgggcg cgagcgcgag acgatttcga agttctcttt
       61 ggcggacatg taatctttct tggcgaaaag aatgcgcgcg aaaatgtagc gaaaaaccgc
      121 attgtcaggc tcttttgtga gggcctgctg catcagggtc gaggccttct cgaggtcttg
      181 agccttgaat gcctcttctg cctgcttgac cagttccgct gcttcagcgt tcgccggtgc
      241 ggctctcagg ggagaggtcg ccatgaccac catgatcacg agacacatga gaatcgctat
      301 tctcttcata gacatatcca tccttatgaa ttcacctatc accatagcat gaaacgacag
      361 cgaattctat tgtctacaac ggatacgagg gagaacggat tgcgcgttac catgaactga
      421 catgatatac tttcccgaga tattcattgc tgtctggtgg acgtgtgaag aacaaacccc
      481 tggctctgat cggcgcgatt ctttttttcg ccggcctgcc ggtgctcctc ttgaatctgg
      541 gttgggagtt cctcaggcgc gaagagcgaa cgcgcgtcgt ctcacagcac tttgaagagc
      601 tcgagcgcta tctgacgcgg atcaaacgcg agggcaacac acaggaatac attcggaaca
      661 cgctgggccg catctgtggt gaactgaacg aagaacagct tgacgcgacc gccgcgcagc
      721 gtgccgttga gacgttgaaa accagtggcc tgtctccgat caaactgtat tttttcgatg
      781 ccaagggcga tggcatcgcg tatccgggac aaccgcgcga atatcagaaa attgtgcaga
      841 agctctatct tgcactttcc gaacctgagg ccggtggcaa cgacaaactg atgcagcagt
      901 accgttcggt tttcgtctct tttctgggta cggtcgagcc gacggagttg atgaatcaaa
      961 agttcgatct ggtgcgggtg gtactcaatg ggaaaacggc tttcttcttt tggggcacca
     1021 tctacgacag cgggtttcgc gggccccatc aggatcagtc gaaaaccagc gacgaccctt
     1081 cgtatcaagg cggggtggtc gccatggtcg aagagcagca catcgaccag aattttgcct
     1141 tggccagcct gctgcaacag ctgaaccgcg atcgacccgc cgggtacggc ttcggttgcc
     1201 tgaacgtgcg acagatcgac cgatctttcc cgaaagaaga cctggaaaaa tgggtgcgaa
     1261 cgccgctgcc gcgattgttt gctcttgtgc agcgtttgca atcgcagttc cagcgttttc
     1321 atgagtattc ggacggcttg ctgacgatcg caccctatga tgacgaaaag gtgctgatcg
     1381 gcctacaacc tcaggtcgtt ccgttaaacc cggggttggg cagactgatt cgcctgcttt
     1441 ctctctttat tcttggattc gtggggtatt gggcctggcg catcgtctac ttcgaacgac
     1501 aggtatttgt gccgattcgt cgcaagctgg tcgtgctgtt tctgtattca accgccattc
     1561 ccatgacggc actcggcctt ctgggatacc agtatgtgac cgaccgtcgc gaagtgctgc
     1621 tgcaagggat ctacaagaaa atggcgggtt ttatcggctc gctggatgac aacttttccg
     1681 tggaactcga caagctgcgt tcttattacc agcggttggc gcggagtaag caggtcactc
     1741 tgagtgactg ggaaggtttc accgaactga catccaagtt gtccgatcgc aacattctgc
     1801 agaacgtctt cattggcaag gccgacggta agatcgtcta catgggggca gggcgaaaag
     1861 gccgcggcaa gaacgatatg tgggccgagc ggctgattcc gacattcatt cgcaagattt
     1921 tttcgcgcca tatgccgcag gtgggcttca atccgaagaa cctgatgcag gatgccatgg
     1981 tcgatactct ggctgacagt ctgtcggatg cgctggccgg tggcggtgga agcggaaacg
     2041 cctttcaacg aatttttgaa cacagcgatg tcatgcacga gatgaatctg ggcaaccgta
     2101 tcaactattt tttcaccagc tttgtccgaa atcctcaagg ggtgatcgac cggctgatgc
     2161 tgatcttgat acccaagaaa cgcatctgta cgcgatatct gcgcagcatc atggcgcgca
     2221 acaagccgcg gcgtcagggc gcagatgact tcatcagact gggctacatt gaggacttcg
     2281 gcgatcgcca ggtcgtgccc cgcgaacttg gcaaatacgg atttgtccgc gaactgttcg
     2341 acaaggtcgt gaacaccaga acgattcaga attccgtaca agtcatggga gggcaaacct
     2401 atctggtggt cgcttctccg ctcaaacgta tcgatgaaca tgtgatgctg gcgttctacc
     2461 cgaaacagat cgtcgatgaa aaagtgagaa atctgaccct gggcctgctg gtcgtcgcca
     2521 cggtcagcgg actgttcgcg gtgttcatag gtttggccct gtcacgtcag tttctggccc
     2581 caattcgcga gctgtcgacc ggcatcaccg cgattgagaa gcgcgacttc cgtcatcaga
     2641 ttcctctgct cgacaacgac gaactgggca gtctgggaac gaccttcaac cagttgatgg
     2701 ccaacctgga agagttgcat gtcgccaaga ttgttcagga aacgctcttt ccgcaggaga
     2761 gcctggagct cggtgactac gagattcatg gaaaaacccg ttcgatgtcc gatcttggcg
     2821 gagattattt cgactatttc cccgttgaga accgctttct ggtaatgttg atcggggatg
     2881 tgacgggcca cggcgtgccg gcagctctgc tgatgaccat ggcgaaaagc gggatttcca
     2941 tcatcggcaa tgatcaagcc accgatgtgg tacgcacgct cgaacgcctc aatcagatga
     3001 tgtttgagac ggtgaaaaag aaacgaatga tgtcgtttct ctacgcggtt ctcgatacgg
     3061 caacgcatca actgcacacg gcgaatgcag gccacaactt tcccttcctg tttcgacgtc
     3121 gcgatcggtc ggcctgtcag atcgagaata cctcttatcc gcttggcgcg cgccgcaagg
     3181 gggtgttcaa acagttgcag ttcgagattc aggcaggcga cgctttggtc atgtataccg
     3241 acggattaat cgagtcgcaa aaccccgctg gggagccgtt ttcctacccg cgcctggaaa
     3301 gcaccattac ccgggttgga acagaagtat catcggctgg aatgcttcac gagctgattt
     3361 ctgccgagtt cgaccggtac atttgcggca agacgcccga agatgatgtc accatgattg
     3421 tcatgcgtcg caaagggtaa gcagcagtac cgctgtggtg gtggttcgcc gaatgcggcg
     3481 aaggcagacc ccgaactgaa tggctttctt attcctcgac cggttcaggg ggtggataca
     3541 ggaacggagc ttcggacacc cggttgcgac cgctttcttt cgaatgatac agggcggcat
     3601 cggccgactt gatgaatgat tccatctcct gggcgcactc cgggaacacc gacacgccga
     3661 agctggcggt gatcttgatc accttgccct catgttcgag atattcggtt tcgatcgtgc
     3721 ggcgaatgcg ttcggcaacg gcggcagcgc catggatatc cgtttcgggc aggacgacag
     3781 cgaactcttc gccaccgtag cgcaccggta gatcatgcgt acgaattgtc ttgcgtagaa
     3841 tcgaagccac tttcttcagc acctcgtcac ccatctgatg gccgtaagaa tcgttgaact
     3901 tcttgaaatg atcgatatcg accatgatca acgacagatg gcggcggaag cggatggcac
     3961 gtttgatttc gtcgaagaga cgctgtttga aataccgatg cagatagaga tgcgtcatgc
     4021 catcggtgat ggcctgctca tacaatttgg cgtt
//
LOCUS       DAORHD010000296         4052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_23990_length_4052_cov_2.667000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000296 DAORHD010000000
VERSION     DAORHD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23990_length_4052_cov_2.667000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKO06_07555"
     CDS             complement(<1..605)
                     /locus_tag="PKO06_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV69533.1"
                     /translation="MADKGTIHQLRNIGIIAHIDAGKTTTTERILYYTGRSYKMGEVD
                     EGTATMDWMIQEQERGITITSAATKCRWRDCDINIIDTPGHVDFTAEVERSLRVLDGG
                     VVVFCAVAGVQPQSETVWRQANRYQVPRIAFVNKMDRVGAYFDRVVEQIKSRLGACPV
                     PVVLPIGAEETFAGHIDLIEMNAHVWGTGRLGDMGDHFEIRA"
     gene            complement(648..1121)
                     /gene="rpsG"
                     /locus_tag="PKO06_07560"
     CDS             complement(648..1121)
                     /gene="rpsG"
                     /locus_tag="PKO06_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNV69534.1"
                     /translation="MPRRGHVTKREQIPDAVYNSKLVTRFVNSLMYSGKRSTACRIFY
                     DAVEKLAKVNNNENPLELFKKALDMAKPIVEVRSKRIGGANYQVPVEVKAERAIALAT
                     RWILGFARARKEKTMADRLAGEMGEILKNTGATMKKRDEVHRMAEANKAFAHYRV"
     gene            complement(1137..1511)
                     /gene="rpsL"
                     /locus_tag="PKO06_07565"
     CDS             complement(1137..1511)
                     /gene="rpsL"
                     /locus_tag="PKO06_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNV69535.1"
                     /translation="MPTINQLIRKARRPVRYKSSSPALQNCPMRRGVCTVVKTTTPKK
                     PNSALRKIARVRLTNKIEVTAYIPGIGHNLQEHSVVMVRGGRVKDLPGVRYHIVRGSL
                     DCAGVANRKQSRSKYGAKRPKK"
     gene            1827..2213
                     /locus_tag="PKO06_07570"
     CDS             1827..2213
                     /locus_tag="PKO06_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV69536.1"
                     /translation="MKFHFAHNNINVLNLEKSVEFYQKALNLTIMQQHSPADGSFKLV
                     YLGDRNTPHLLELTWLRDRHEPYNLGDNEFHLAFSVDDFSAAHERHEHMGCICYENPT
                     MGIYFIEDPDGYWLEIIPASLRLGNA"
     gene            2213..2611
                     /locus_tag="PKO06_07575"
     CDS             2213..2611
                     /locus_tag="PKO06_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HNV69537.1"
                     /translation="MTGSSIVLPSGGLEGLEHQLLICALEVQKNLGQTVPENACRDCF
                     AFELEKRNIPFTARVELLNHSGSEPLPSGYRVDFVVAGQVIVEVRCRPSLPHNLTDQY
                     RSYLRLSGLSAAVIINLQAAPPQLLTRITR"
     gene            complement(2601..3131)
                     /locus_tag="PKO06_07580"
     CDS             complement(2601..3131)
                     /locus_tag="PKO06_07580"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNV69538.1"
                     /translation="MYKGEKTILRAYEKRDLEVAHRLFNDLEVQKLLNPGVILPLSLE
                     EEQKFIETNASAKESRYNFAVDTLDGVYIGGGGYFDFNRRNGTTFIGIAIAHRDYWGK
                     GYGSDALQVLLRFLFLELNVRKILLNVFAFNARAIACYQRLGFREEGRLKQQVYRDGT
                     FHDELIMSLFREQFNA"
     gene            complement(3219..>4052)
                     /locus_tag="PKO06_07585"
     CDS             complement(3219..>4052)
                     /locus_tag="PKO06_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV69539.1"
                     /translation="ALRSLELFECRRPKKEAVISDDSGVVRISGNTVSLADENGNEKP
                     VRLQGVTGLIVHDGEYIEAGDSITEGKTFPKKLVKCVGLNHVRRYLVDEIQRVYREQG
                     VSINDKHLEIIIRQMLRKVVISDPGETDFLPNETVHVKAFEESIARMESEKRKKPTGY
                     PMIQGITKASLTTDSFISAASFQETTRVLTKAAIKSKVDYLRGLKENLIIGKLIPAGT
                     GLAISKKINFKNLPIEIEKTETTDGSEELFNTQEIQEEIDSELFVKKADTGDDAEETE
                     E"
BASE COUNT          849 a         1172 c         1047 g          984 t
ORIGIN      
        1 gcacggatct cgaaatgatc gcccatatcg ccaagacgac ccgttcccca cacgtgggcg
       61 ttcatttcga tcaagtcgat atgaccggca aacgtttctt cagctccgat cggcagaacg
      121 accgggaccg ggcaagcgcc caaccgcgat ttgatctgtt cgaccacgcg atcaaagtag
      181 gctccgaccc ggtccatttt gttcacgaaa gcgatgcgcg gaacctgata gcggttggct
      241 tgacgccaca ctgtttcaga ttgcggctgc acccccgcaa cggcgcaaaa caccacgact
      301 ccaccgtcca gcactcgcag agagcgctca acctcggccg tgaaatccac gtgcccgggt
      361 gtgtctatga tgttgatgtc gcaatcacgc cagcggcact ttgtcgccgc agaggtgatc
      421 gtgattcccc gctcctgttc ctggatcatc cagtccatgg tggcggtacc ttcatcgact
      481 tcacccatct tgtaactgcg ccccgtgtaa tacaggatgc gttccgtggt cgtggtttta
      541 ccggcatcaa tgtgagcaat gatgccgata ttccgcaact ggtgaatggt acccttgtca
      601 gccatggtga atctatttta ccgattgaag cgggattcgt ctaggggtca cacgcgataa
      661 tgcgcgaacg ccttgttggc ttcggccatg cgatgcactt catcgcgctt cttcatggtc
      721 gcgccagtgt tcttgaggat ttcacccatt tcgccggcca gacggtcggc catggtcttc
      781 tctttgcggg cgcgggcaaa accgaggatc cagcgggtgg cgagagcgat cgcacgctcg
      841 gccttcactt cgaccggaac ctggtagttg gcgccaccga tgcgcttgga gcgcacttca
      901 acgatgggct tggccatatc gagcgccttc ttgaagagct caagcgggtt ctcgttgttg
      961 ttcaccttgg ccagcttctc gaccgcatca tagaaaatgc ggcaagcggt gctgcgcttt
     1021 ccgctgtaca tcaggctgtt gacgaaccgg gtcaccagct tggaattgta cacggcatcg
     1081 ggaatctgtt cgcgcttagt tacgtgtcca cgtcgtggca tgtctcaaac tccttcttac
     1141 ttcttcgggc gcttcgcgcc gtacttggag cggctctgct tgcgattggc cacgccggcg
     1201 cagtcgaggg aaccgcgaac gatgtggtag cgaacgccgg gaagatcttt cacgcggccg
     1261 ccgcgtacca tgaccaccga gtgctcctgc aggttgtgtc cgatacccgg aatgtaggcc
     1321 gtgacctcga tcttattggt caggcgcacg cgggcaatct tgcgcagcgc ggagttcggc
     1381 ttcttgggcg tcgtcgtttt tacgaccgtg cagactcccc ggcgcatggg gcagttctgc
     1441 aatgcgggag aggaactctt gtaccgcacc ggacgccgag ccttacgaat cagttgattg
     1501 atagtcggca ctttcgtcac ctaccttgct tcatgctcgt cccctgcgga tctcgcgaaa
     1561 agacctaacg cgggaacagt aaagtaggat gttagcacat tggaaggccg ctgtcaatgt
     1621 acattttccg cgaatcagca aacgaaaaac atggtagtat gacgttgctc cgcgcatgac
     1681 gttccccccg caacagagca atatctgatg gaatgcaggt ttatctgtgt gtccgagcga
     1741 ccggcacgct tcgaaatcaa ccctcggcag aagctcgttc tcgtgatcac aggcatggca
     1801 actatttgtt cttggagaat ctgacaatga aatttcactt tgcccacaac aacatcaacg
     1861 ttctcaatct tgaaaaaagt gtcgaattct accagaaggc cctgaatctc accataatgc
     1921 agcaacattc gcctgctgac ggcagcttca aactggtata tcttggtgac cgcaacactc
     1981 ctcacctgct cgagctcacc tggctgcgcg atcgccatga gccgtacaac ctcggcgaca
     2041 acgagtttca tctcgctttt tcggtcgatg acttctccgc agctcatgaa aggcatgaac
     2101 acatggggtg catctgctac gagaatccaa ccatgggaat ctacttcatc gaagaccctg
     2161 acggttactg gctggagatc attcccgcct cgctgcggtt ggggaacgcc tgatgacggg
     2221 ctcttccatt gtgttgccaa gcgggggcct cgagggcctc gaacatcagc tgctgatctg
     2281 cgcgctcgag gtgcaaaaaa acctcggaca aacggttccg gaaaacgcct gtcgcgattg
     2341 tttcgccttc gaactggaaa agcgcaacat tcccttcacg gcccgggttg agttactcaa
     2401 tcattccggg tccgagccac tgcccagcgg atatcgcgtc gatttcgtcg tggccgggca
     2461 agtcatcgtt gaagtccgct gtcggccctc tctcccgcac aacctgaccg accaataccg
     2521 ctcgtatctt cgtctgtccg ggttgtcagc cgccgtcatc atcaatttgc aggctgcccc
     2581 accccagctg ctcacccgta ttacgcgttg aattgttcgc gaaagagact catgatcagc
     2641 tcgtcgtgaa aggtgccgtc ccggtacacc tgctgtttca agcgtccctc ctcgcgaaag
     2701 cccagtctct ggtaacaggc tatcgcccgc gcattgaacg caaacacgtt caacagaatc
     2761 ttgcgtacat tcaactccag aaaaaggaac cgaagcagta cctgcagcgc atctgatcca
     2821 tatcctttgc cccaatagtc ccgatgcgcg atcgcaatgc cgataaaggt cgttccattt
     2881 cgacgattga aatcaaaata accacccccg ccgatgtaga cgccatcaag ggtatctacg
     2941 gcgaagttgt atcgactttc cttggctgag gcattcgttt caataaactt ctgctcttct
     3001 tcgagagaaa gcggaagaat aacgccagga tttaacagtt tttgcacttc gagatcattg
     3061 aacagtcgat gcgccacctc gagatctctt ttctcatagg cacgtaaaat agttttctca
     3121 cccttgtaca tagacacttt cctgtgaatt ttcagagatg gaaaaagccg ggctctttct
     3181 aggagagccc ggctttctgg ttcaaacgat actatcgctt attcttcggt ttcctcggcg
     3241 tcatcaccgg tatcagcttt cttcacaaag agttcgctgt cgatctcttc ctgaatctcc
     3301 tgagtgttga acaactcttc gctgccatcg gtcgtttcag tcttctcgat ttcgatcggc
     3361 agattcttga agttgatctt tttgctgatt gccagaccgg ttccggccgg aatcagcttc
     3421 ccgatgatca ggttctcttt caaaccgcgc agatagtcga ccttcgactt gatggcggct
     3481 ttggtgagaa cgcgggtggt ttcctggaac gatgcggcgg agatgaagct gtcggtcgtc
     3541 aacgaagcct tggtaatacc ctgaatcatc gggtatccgg tcggcttctt gcgcttctcg
     3601 ctctccatgc gggcaatcga ttcctcgaag gccttcacat gcactgtctc gttgggcagg
     3661 aagtcggttt cacccggatc actgatgacg actttacgca gcatctggcg aatgatgatt
     3721 tcgagatgct tgtcgttgat cgaaacgccc tgttcacgat acacgcgctg aatctcgtcg
     3781 acgagatagc ggcgcacgtg attgagaccg acgcatttga ccagcttctt cgggaaggtt
     3841 ttgccttcgg tgatcgaatc gccggcttcg atatactcgc cgtcgtgcac gatcagccct
     3901 gtcaccccct gcagacgcac cggtttctcg ttgccatttt cgtcggcgag agaaacggtg
     3961 ttgcccgaga tgcgcaccac acctgaatcg tcagagatga cggcttcttt cttcgggcgg
     4021 cggcactcga acagttccag cgagcgcagg gc
//
LOCUS       DAORHD010000297         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24009_length_4050_cov_3.919650, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000297 DAORHD010000000
VERSION     DAORHD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24009_length_4050_cov_3.919650"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKO06_07590"
     CDS             complement(<1..239)
                     /locus_tag="PKO06_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HNV69540.1"
                     /translation="MSLRINQNVLAIQTQGMLSSTSGRLEKVVEKLSSGLRINRAADD
                     AAGLAISEKIRRQIRGLSRAVMNAQDGISMIQTAEG"
     gene            complement(390..2615)
                     /locus_tag="PKO06_07595"
     CDS             complement(390..2615)
                     /locus_tag="PKO06_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV69541.1"
                     /translation="MSLRINQNVLAIQTQGMLSSTSGRLEKVVEKLSSGLRINRAADD
                     AAGLAISEKIRRQVRGLSRAIMNAQDGISMIQTAEGALGESHSILQRMRELAIQASSD
                     TLTSGDRLEIQKEVNQLRDDLNRIARNTEFNTKKLLDGSQTALVSGSSDSVKGVVVGS
                     AGAVGGDYEVSLTLLQGGISEMQRSQTFLKNDGSGQIAQGSTSLQSIAQFYDANGVFV
                     LQSPQTLTINGSGKSTSITVDGQMSLDQLAAAVQNAIVGASGLDMANTRIEVVNTATT
                     GLAGMGGYLQTISGSIGQAGQISFSGDQALMDALGMTVTRTAVENQIEMTLRDANGNT
                     KTALTTDSRVTGLLSGIDLAFTSQAAQVAGTKGLQQGLELTNSVVLTISAGTTNVTVG
                     VVSGDWTMEGIARAINDQVVTVIEGLSAAVVDGQIRISYDRPATAPVSIGTTIRISGA
                     NNEAVATLGIVNGTYSGFVQGQKDEAKSVWGFSMYNGTISATTATFGISDGTKAALTI
                     TAFVTTSVGSTADMVRFTTFQASVNRLLESTATSTTRAVRVDQVGATIAFTALRVGQE
                     NPDNAAVIRSMVTFNGGNLVNQLFDLSNGTALGSGDKNFRVHVVDNTPKFQIGGDPGQ
                     SMAVGFASMNAEALGVQSLDMSSISGAERSLAKVNAAIDKVSAERSKLGAFQNRLEYS
                     INNLRNTYSNLSAAESRIRDADIALEMIEFTRNQIVNQSGTAMLAQANLLPSGVLELL
                     R"
     gene            complement(2840..>4050)
                     /locus_tag="PKO06_07600"
     CDS             complement(2840..>4050)
                     /locus_tag="PKO06_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV69542.1"
                     /translation="LTTDSRVSGLLNGIDLAFTSQAGQIAGTKGLQEGLAFTADLTFA
                     ISAGGVNTNIVISGGSARFGYSMEGIARSINDQVAGVLGLSAAVVDGQIRVSYERPAT
                     AAASIGTTIQIANATTNGVVDVLGIVNGTYSGFVQGQKNEGYKVWGFTNYATAAQAVT
                     FRMNDGNVPDVEVTAYTSVTIVSSADLYKFDVWQATVNYQLATADVAVRVDQVGSTVA
                     FTALRVGRENPDSAAAIRSVVSLDADAEFNGLFDIQDGTAMGSGDKNFRVHIVNNSPN
                     FQIGGDPGQKMQVNFANMNADALGVQALDLTTVSAAAKSIARINSAIDQVSAERSKLG
                     AFQNRLEYAISNIRNTYTNLSAAESRIRDADVAQEMIEFTRNQIVNQSGTAMLAQANL
                     LPQGVLQLLS"
BASE COUNT          849 a         1279 c         1001 g          921 t
ORIGIN      
        1 ccctcagccg tttgaatcat cgagatgccg tcctgcgcat tcatgacagc gcgtgacaaa
       61 ccccgaatct ggcgacgtat tttttcgctg atcgccaaac ccgcggcatc atcggcagcg
      121 cgattgatgc gcagcccgga actcagtttt tcaacgacct tttccagtct tccagacgta
      181 ctcgatagca tgccctgggt ctggatcgcc aggacattct gattaattcg tagcgacata
      241 ctgtttcctc catgaactaa cctccgtgct tccctgcaac ggaggcgtga ttacgccctt
      301 tcgctcccgg catcacctcc tgcatttcag atcgcaaggc cgaccggtga ggggacgagc
      361 ccctcaccgg aactttcacc ccagactgtt tatctcagca gttcaagaac cccgctcggc
      421 aacagatttg cctgagccaa catggccgtt ccggactgat tcacgatctg attgcgtgtg
      481 aactcgatca tttccagcgc aatatcagca tctcgaattc tcgactcagc ggctgacagg
      541 ttgctgtagg tgttccgcag gttgttgatc gaatattcca gacgattctg gaaagcgccc
      601 aacttcgagc gttctgccga cactttgtca atcgctgcgt tcaccttggc cagtgatcgc
      661 tcagcaccgc tgatcgacga catatccagg ctttgcacgc ccaacgcttc cgcattcatg
      721 ctggcaaagc ccaccgccat cgattgtccg gggtcaccgc caatctggaa cttcggcgta
      781 ttgtcgacga catgaacccg aaagttcttg tcaccgctcc ccaacgccgt gccgttcgac
      841 aggtcgaaca gctgattcac cagattcccg ccgttgaatg tcaccatact gcggatcaca
      901 gctgcattgt ccgggttctc ctgaccaacg cgaagagccg taaaggcaat cgtcgcccca
      961 acctggtcga cccgaacagc ccgcgtcgtc gacgtggcag tactttcgag cagtcgattt
     1021 acagacgctt ggaatgtggt aaagcgcacc atatcggccg tactacccac actggtcgta
     1081 acgaaagccg ttatagtgag tgccgccttt gttccatcgc tgattccaaa cgttgcagtc
     1141 gtcgccgaaa tcgttccatt gtacatggag aagccccaga cggatttcgc ttcgtctttc
     1201 tgaccctgca caaatccgct gtaggtgcca ttgacgattc cgagggttgc gaccgcctcg
     1261 ttattcgcac ccgagatccg tatcgtggtc ccgatgctca caggagcggt agccgggcga
     1321 tcatacgaga tacgaatctg cccgtccact acggccgcac tcaacccttc gatgaccgtg
     1381 accacctgat cattaatggc tcgagcgatt ccctccatcg tccagtcacc agatacaaca
     1441 ccaacggtga catttgtggt cccagctgag atggtcaaga ccaccgagtt tgtgagttcg
     1501 agcccctgct gcaagccctt ggtgcccgca acctgcgccg cctgagaggt gaacgccagg
     1561 tcgatacctg acagcagtcc agtgacccga ctgtccgtgg tcaaggcggt tttcgtattg
     1621 ccattcgcat cacgcagcgt catctcaatc tggttttcca cggctgtccg ggtaacggtc
     1681 atgcccagag catccatgag agcctgatcg cccgagaacg agatctggcc tgcctgacca
     1741 atacttcccg agatcgtctg caggtaaccg cccatgccgg cgagaccggt cgtagcggtg
     1801 ttcaccacct cgattcgagt gttggccata tcgagaccac tggccccgac gatcgcgttc
     1861 tgaacagccg cagccagttg atctaagctc atctgtccat caaccgtgat cgaggtgctc
     1921 ttgccactgc cgttgatggt cagcgtttgc gggctctgca atacgaacac gccgttcgca
     1981 tcatagaact gcgcaatgct ttgcagcgac gtactgccct gagcaatctg cccactgccg
     2041 tcattcttca gaaaggtctg cgaacgctgc atctcggaga tgccgccctg cagcaaggtc
     2101 agactgactt cgtagtcacc gccgaccgcg ccggcagagc ctacgaccac acccttaacc
     2161 gagtctgaac ttcccgatac caatgcggtc tggctgccat caagcagctt cttggtattg
     2221 aattccgtgt tccgggcaat gcggttcagg tcatcccgca gctggttcac ttccttctga
     2281 atctcgaggc ggtcaccact cgtcagggta tcactgctgg cttgaatcgc cagttcccgc
     2341 atgcgttgca gaatactgtg cgattcaccc agcgcgcctt cagccgtttg aatcatcgag
     2401 atgccatcct gcgcattcat gatggcgcgc gacaaaccgc gaacctggcg acgaatcttc
     2461 tcgctgatcg ccaaacccgc ggcatcatcg gcggcacgat tgatgcgcag accggaactc
     2521 agtttttcaa cgaccttttc cagtcttcca gacgtacttg aaagcatgcc ctgggtctgg
     2581 atcgccagga cattctgatt gatacgtagc gacataatgt ttcctccatg aactaacctc
     2641 cgtgcttccc tgcagcggag gcgcgattac gccctttcgc tcccggcaac ctccttctcg
     2701 tctcgattgc tgacgcaacc ggcccttcgc cagatgggcg aaatcccccc ttcccttggg
     2761 ggtgcgatca gatcacagcg gaccgcggat agagctgctc gctctatccg ccttctgact
     2821 cgtctaattc aaaaccgtcc tagctcaaga gctgcaaaac tccctgcggc aacagatttg
     2881 cctgggccaa catcgccgtc cccgactgat tcacaatctg gttgcgcgtg aactcaatca
     2941 tttcctgcgc cacatctgcg tcacgaatgc ggctttcggc agccgacagg ttcgtgtagg
     3001 tgttgcgaat gttgctgatc gcatattcca aacggttctg gaacgcaccc agtttcgaac
     3061 gttccgccga cacctggtcg atcgccgagt tgatcctggc aatcgacttc gccgcggcgg
     3121 aaactgtggt gagatcgagc gcttgcacgc caagagcatc cgcgttcatg tttgcaaaat
     3181 tcacctgcat cttctgccca ggatcaccac caatctggaa attcggcgag ttgttcacga
     3241 tgtgaactcg gaagttctta tcgccactcc ccatggccgt tccatcctgg atatcgaaca
     3301 gaccgttgaa ttcggcatcc gcatccagac tgacaacact gcgaatggcc gcggcgctat
     3361 ctggattttc cctaccaact ctcaatgcgg taaacgctac cgtcgagccc acctgatcaa
     3421 cgcgaacggc aacatctgca gtcgcaagtt gataattcac cgttgcctgc cacacgtcga
     3481 acttgtacaa gtctgcagaa ctgacgatcg ttaccgaagt gtaagccgtg acttcgacat
     3541 caggaacgtt gccatcattc attctaaagg tcaccgcctg cgcggcggtc gcatagtttg
     3601 tgaaacccca gaccttgtag ccctcgttct tctgtccctg cacaaatccg ctgtaggtgc
     3661 cgttcacgat gcccagtacg tcgaccacac cgttggtcgt cgcattggca atttgaatgg
     3721 tcgtaccgat ggaagccgcg gccgttgccg ggcgctcata cgaaacgcgg atctggccat
     3781 cgacaacggc ggccgacaga ccaagcacgc cggctacctg atcgttaatc gagcgcgcaa
     3841 tgccttccat gctgtagcca aatctcgctg atccaccaga gatgacgata ttggtgttta
     3901 cgcccccggc actgatcgca aacgtgaggt cggccgtgaa cgcgagtcct tcctgcaatc
     3961 ccttggttcc ggcaatctgc cctgcctgcg aggtgaacgc cagatcgatg ccgttcaaca
     4021 ggccactaac gcgactgtca gtcgtcagcg
//
LOCUS       DAORHD010000298         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24056_length_4046_cov_3.788775, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000298 DAORHD010000000
VERSION     DAORHD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24056_length_4046_cov_3.788775"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1277
                     /locus_tag="PKO06_07605"
     CDS             <1..1277
                     /locus_tag="PKO06_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV69543.1"
                     /translation="PGGKREAGESFEQCLMRARSHNRTLLTQKARIAAADARLKQVKA
                     SQRPTVNAQWSDTSYNAPSSPTAAREQHLHRLTVSDTVAPFGRYRSQKRAAEAAQLAT
                     RHEESRAEQDIDFQVAKLFYDLLLAQQLVRVASDSVDQLTSHRDNTAALVRAGSAPKF
                     DLLRAEVQLASARPPLIKAQSTITNAMADLMNLLGLDPQLSPELIGSFPEQLPEPPDE
                     REAITVAHERRPDLAAAREWWRSQQHSVQAARQALQPTVQLAQTWDHSKGLRTPVHSY
                     QQSSFTQLNLSWPMFDSGLTRARVHEARAGATQAALAAESAQANAIVEVRKALSAWHE
                     AVEVLRSQEKNVEQATEALAIAKAAYKAGARTALDVLDAQVALTQARTLHFQALYART
                     LAHLSLRRASGLPFSLTETFPLANASSDRSHP"
     gene            1277..2365
                     /locus_tag="PKO06_07610"
     CDS             1277..2365
                     /locus_tag="PKO06_07610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV69544.1"
                     /translation="MSSSPTRTFRIRFPGCAFAVLLVVCGSLLQAAENAPSVPSERGS
                     PVPLPTLAAPVDATPPASQPSTMTEIASATIPELIGELSPYRKVSLGFRINGRVWQMN
                     LLAGDLATPGSVIGTLEPQDLELAQEQSEASLAVLQSKLRMQETGARPEEKRQSEEAV
                     RQAKANLENAQSDFERTSQLFTTGAVSRQALDAAVARKDVAAAQYQSVLNARALVFQG
                     PRVEEKESTRAQIRSQEAALKLAKLQLSYCRLEAPFPAIVAARLQDEGAWVTPSTPVY
                     TLVQIDPLFATAECPEQFVPRLKIGMRAEITVDALPGETFSGLLDRIPGVIDSKSRTA
                     RVEITLNNPHYRLKPGMFARIRLFDAER"
     gene            2376..>4046
                     /locus_tag="PKO06_07615"
     CDS             2376..>4046
                     /locus_tag="PKO06_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69545.1"
                     /translation="MNLANICIHRPVFTVMLMAALVVLGLFSYTGLGLDLYPKIDFPV
                     VSIAATLPGAGPEEIETQLAKPIEEAISTLGGIDIMTTSCVFGMCRIVVRFDLEKNID
                     VAAQEVRDRVARILKTLPEGTESPIIEKMDPDASPVITVVLSSPMPLRELTYFARKQI
                     KEPLEAIEGVGAIQVVGGREREIHAILDAKKLAAFQTSVTQVRDALKGQNIDIPGGNI
                     TNGPNESILRTLGRIQTPEEFGDVIVQVQNGIPLRLKDLGTIIDHEEEARTTARLNGK
                     TALALVVQKKSGTNTVQVIDTIKQRLKDLSKEFPQGLTYAIIRDQSQFIKASLHELNL
                     HLVLGAILASAVVYLFMQNLVSTLISALAIPISLISTFLLMRLMGFTLNNMSMLGLTL
                     AVGIVIDDAIVVLENVYRHMEEKGSDAMTAASEATSEISLAVLATSLSLAVIFVPVAF
                     MQGIIGRFLNNFGLTIAFSILISIFVSFTLTPMMCSRLFRYRFARGSGHSKESAFYRV
                     IDRVYGRLLRFSLDHRWVIILLCILSLVALKPMGAFIRSDFVPVDDTGE"
BASE COUNT          827 a         1318 c         1084 g          817 t
ORIGIN      
        1 ttcccggagg aaaacgcgaa gccggcgaat cctttgagca atgcctcatg cgcgcccgtt
       61 cccacaatcg gactctgctc acccagaagg cgcgcatcgc agccgcagac gcccgcctga
      121 agcaggtcaa agccagtcaa cggcccaccg tcaacgcgca atggagcgac acgtcctaca
      181 atgccccttc gagcccaacc gcggctcgcg aacaacatct tcatcgcctg accgtttcag
      241 acaccgtggc ccccttcggt cgctatcgtt cgcagaaacg agcggccgaa gcggcacagc
      301 tggctacccg tcatgaagaa agccgggccg agcaggacat cgattttcag gtcgccaagc
      361 ttttctacga tctgctgctc gcccagcaac tggtacgagt ggcctcagac tccgtggatc
      421 agctcacgtc acaccgcgat aacaccgccg cacttgtccg agctggctca gcgccaaagt
      481 tcgatctgct gcgggctgaa gtgcaactgg catcggcacg cccaccgttg atcaaagcgc
      541 aatcaaccat caccaacgcc atggccgacc tcatgaacct gctcggactc gaccctcagc
      601 tctcgcctga actgatcggt tcatttcccg agcaactgcc ggaaccgccg gacgagcgag
      661 aggccatcac cgtcgctcat gaacgccgcc ccgacctggc agccgcgcgt gagtggtggc
      721 gcagccaaca gcattcggta caagcggctc gacaagcatt acagccaacg gtgcagctgg
      781 cccagacctg ggaccacagc aaaggtttgc gcaccccggt gcattcctat cagcagtcct
      841 cctttacgca gctcaatctg tcgtggccca tgttcgactc agggttgacg cgagcccgcg
      901 tgcacgaagc ccgggcgggt gcgacacaag ctgctttagc cgcggaatcc gctcaggcaa
      961 acgcgatcgt cgaggttcgc aaggctcttt ccgcctggca tgaggccgtc gaggtgctgc
     1021 gcagccagga aaagaatgtc gagcaggcaa ccgaagcact cgctatcgcc aaagccgcct
     1081 acaaagccgg agcacgcacc gcgcttgatg ttctcgatgc ccaggttgcc ctgacccagg
     1141 ctcgcaccct gcatttccag gctttgtatg cgcgcacgct ggcccacctc agtctccgac
     1201 gcgccagcgg gctgccattt tctctcaccg aaacctttcc acttgccaac gcttcgtcag
     1261 acaggagtca cccctgatgt catcctcccc cacccgcacg ttccgcatcc gctttcctgg
     1321 ctgtgccttt gcggtcctcc ttgtggtctg cggcagtctg ctgcaggctg ccgagaatgc
     1381 cccgtcggtt ccgtctgagc gtggttcgcc cgtgcctctg ccgacactgg ccgcgcctgt
     1441 cgacgccact ccgccagcca gccaaccgtc aacgatgacg gaaatcgcct ccgcgacgat
     1501 tccggaattg atcggtgaac tgtctccgta ccggaaggtt tcactcggct tccgcatcaa
     1561 tggccgagtc tggcagatga atcttctcgc cggcgatctc gccacccccg gcagcgtcat
     1621 cggaactctc gaaccgcagg atctcgagct tgctcaggaa cagagtgaag cgtctctggc
     1681 tgtgctgcag tccaaactgc gtatgcagga gacgggagcg cgaccggaag aaaagcgaca
     1741 gtccgaggaa gcggtgcgcc aggcaaaggc caaccttgaa aacgctcagt cagattttga
     1801 gcgcacgtca cagctgttca cgaccggagc cgtatcacgt caggctcttg acgccgccgt
     1861 cgcacgcaag gatgtggcgg cagcccagta tcagagtgtc ctcaacgccc gcgccctcgt
     1921 ctttcaggga ccgcgtgtcg aagaaaagga atcaacccgg gcgcagattc gctcgcagga
     1981 agcggcttta aagctggcca aactgcagct ttcctactgt cgactcgaag ctccctttcc
     2041 cgcgatcgtg gcggctcggc tgcaggacga aggcgcgtgg gtcaccccgt ccacaccggt
     2101 ctataccctc gtacagatcg atccgctgtt cgcgacagcc gagtgtccgg aacagttcgt
     2161 tccccgactc aagattggca tgagagcgga aatcaccgtt gacgcgcttc ctggagaaac
     2221 gttttcaggc ctgctcgacc gcattcccgg cgtcatcgac agtaaatcac gaaccgcgcg
     2281 cgtcgagatc actctcaaca acccgcacta ccgtttgaaa cccggcatgt tcgcgcgtat
     2341 ccgcctgttc gacgcagaac gctgagaatc aacccatgaa cctcgccaac atctgtattc
     2401 accgccccgt cttcactgtc atgctgatgg cggcactcgt cgtcctcggc ctcttttcct
     2461 acaccgggct cggactcgac ctgtacccga aaatcgactt tcccgttgtc tcgatcgccg
     2521 cgactcttcc cggcgccgga cccgaagaga tcgagaccca gctggccaag ccaatcgaag
     2581 aggcgatcag tacgctgggc ggcatcgaca tcatgaccac ttcgtgcgtg ttcggcatgt
     2641 gtcgtatcgt cgtacgcttc gatcttgaaa agaatattga tgtcgccgcg caggaagtcc
     2701 gcgaccgtgt cgcccgcatt ctcaaaactc tgccggaagg caccgagtcg cccatcatcg
     2761 agaagatgga tccggacgcc tcaccggtca tcaccgtggt tctctcctcg ccgatgcccc
     2821 tgcgcgaact cacgtatttc gcccgcaagc agatcaaaga gccacttgaa gccatcgaag
     2881 gagtcggagc gattcaagtc gtcgggggtc gggaacgcga gattcacgcc attctcgacg
     2941 cgaagaagct ggccgcgttt cagacgtcgg tgacccaggt tcgagacgct ctgaaagggc
     3001 agaacatcga tattccgggc gggaacatca ccaacgggcc gaacgagagc attctgcgca
     3061 cgctgggacg catccagact cccgaggaat tcggcgacgt gatcgtccag gttcagaacg
     3121 ggattcctct gcgtttgaag gaccttggca cgatcatcga tcacgaagaa gaagcgcgaa
     3181 ccacggcacg tctcaacggc aagacggctt tggcgctcgt ggtacagaaa aagtccggca
     3241 ccaataccgt acaagtcatc gacacgatca aacagcgtct gaaagacctt tcgaaggagt
     3301 ttccgcaagg ccttacgtat gccatcattc gtgatcagag ccagttcatc aaagcgtcac
     3361 tgcacgaact caatctgcac ctggttctcg gcgcgattct cgcatctgcc gttgtctatc
     3421 tgtttatgca gaacctggtt tcgacgctga tttcggctct ggccattccg atctcgctca
     3481 tttcgacctt tctgctgatg cgcctgatgg ggttcaccct gaacaatatg tcgatgctcg
     3541 gcctcactct ggccgtcggc atcgtcatcg acgacgcgat cgtcgtgctc gaaaatgtgt
     3601 atcggcacat ggaagagaaa ggttccgacg cgatgactgc cgcttcagaa gctacttccg
     3661 aaatttctct ggcggtactc gcgacttcgt tgtcgctggc ggtcattttc gttccggtgg
     3721 cattcatgca gggcatcatc ggccgctttc tcaacaactt cggcctgacc atcgcctttt
     3781 cgattctcat ttccatcttc gtgtcgttca cactgacccc gatgatgtgt tcgcgtctgt
     3841 tccgctatcg gttcgcgcgc ggctcaggtc atagcaaaga atccgcattt taccgggtga
     3901 tcgaccgggt ctacggacgc ctgcttcgct tctcgctcga tcatcggtgg gtcatcattt
     3961 tactctgcat cctgagcctg gttgcgttaa agcccatggg ggcgttcatt cgcagcgatt
     4021 tcgtccccgt ggatgacacc ggggaa
//
LOCUS       DAORHD010000299         4039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24119_length_4039_cov_4.164157, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000299 DAORHD010000000
VERSION     DAORHD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24119_length_4039_cov_4.164157"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2822)
                     /locus_tag="PKO06_07620"
     CDS             complement(156..2822)
                     /locus_tag="PKO06_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69546.1"
                     /translation="MRIRRFHRVLMGILALLLLIEPTVIEVVRAENTPQRPISREVIE
                     NGIEKQDVEDYLGRVTADDIMLPKEMLLQLLAAKGGLEGDCDQAVIEANSVSDFAQML
                     AGIMGVLETPDLLCYYTLNWSPEESEGVAGAVGGGKDVFEGLAGVPAMIKKWGQRLEL
                     FSHNIGRFYGRKGRNLGKVIGDSMQFMAKRSKQIKKMLDKIVSNKVLKFMENVASVDY
                     ENAGETGKYLKKFWAAKFNQDAGKNFVSRGSNPVGIGKTVGVGLQILGGVLAGISIAN
                     SNERRMGGAHNWFSYEMTKNYVDIALAAVFVLMLCFAALSPIGWAVAAIEAAWEVFCM
                     IGDAVGAYAKKWHAAYQDSFWFLKQKDKDFQKLIDNWKNPDLLHQDEMSLALLKAIAM
                     RKKLEVEKMEGEEKKAFESLLEKIEQQGVLTSYYYKQPWKMKDFSLETSMALWKYKAT
                     YMSWQPSQQEVEEEKDKGWFDKFTDAINPSTWIEKGIELGARIKTTVFEDSLEEKIRK
                     NPNIRHVDFNPDFALLKKYQSYLASNKIQPGINVESLIGLRIEQAPFNYIPLVEVAND
                     KLNPEILVESFMVDTVFLGALELKSFSEALKKLAEDAEKFRKGMSDVAKLKRLHGERR
                     ELRQTYKELLTAYLADQDVDRALLRKLRYTPPRNTDSNQEQSASSGSGEPKAKDVLLA
                     LKDRINQDLLVVPKMNGDKMIDLMKMALDIKKMRDQSAMINSTLERMDCQPIVEQIHN
                     PTLKKYITDGEFLNVNKGLLSGVWNWFAGLKSGKTEYDDNRNLARSYYGEFSEEVEKA
                     YDRFKSVWGKTIEEAIIEVINEHYAFRDDILRVVVDNQTKLEGDGIDLKVIYDLDSPD
                     IYPTERFKLPCTLKALDPDSVKIPETAAVDEP"
     gene            complement(2873..3895)
                     /locus_tag="PKO06_07625"
     CDS             complement(2873..3895)
                     /locus_tag="PKO06_07625"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69547.1"
                     /translation="MQRRFLSRMGIGIILCGALWLPPAVGSAASLDEATALEKAGKTE
                     EAMVQYRELIKDKPSEELYKKAVAVAGRRQRYDEARQLLEQALQQFPESLSLRNQMAM
                     IRFRLGQKTESLKELEVVLGKDPENRFAKELKAQIDKGRGVSPAPQSKPDVAVAAPVE
                     PQAKSTKSLEEQKKLAKDLYKEMAALDRWALDDFIRLHQQVINDCPDTHWAEESYWRL
                     SSLYMSGYSEPDWTRIIETLEAFLQKYPNTKLKSEVHDRLLLAYKETGNNDKLLEISQ
                     TELSTPPSGVPDTRYLGIAMDFAEAQAARGNQAEAKKMFEQIVQWDNGRDTFIARLAR
                     SKLNGN"
BASE COUNT          779 a         1232 c         1030 g          998 t
ORIGIN      
        1 cttccatttg ctgggtcacg aatgcagcca gcttttccag agcttcattt tcgttactgg
       61 tttcatcagc catcgttcga tctccttttc gatcttccaa ataaggaagg gggcagcccg
      121 ggtctcgagc taccccctct gtgtctcaca tcacgttagg gttcatccac ggcggccgtc
      181 tcaggaatct tgacggaatc gggatcgaga gctttcaggg tgcacggcag cttgaagcgc
      241 tcggtcgggt aaatgtccgg gctgtcgaga tcgtagatga ctttcagatc aatgccatcg
      301 ccttccagct tggtctgatt atcgacgacc acgcgcagaa tgtcatcgcg gaaggcatag
      361 tgttcgttga tcacttcaat gatcgcttct tcgatcgtct tcccccacac ggacttgaaa
      421 cggtcgtatg ccttctcgac ttcctcggag aactcgccat aatagctgcg cgccaggttc
      481 cgattgtcgt catattccgt cttccccgat ttcaagccgg cgaaccagtt ccagactccc
      541 gacagcagcc ctttgttcac attcaggaac tcgccatcgg tgatgtactt tttcaacgtc
      601 ggattgtgaa tctgctcgac gatgggctgg cagtccatgc gctcgagcgt cgagttgatc
      661 atcgccgact gatcgcgcat cttcttgata tctagcgcca tcttcatcag atcgatcatc
      721 ttgtcgccat tcatcttcgg cacgaccaac agatcctgat tgatacgatc tttcagcgcc
      781 agcagcacat ccttggcctt gggctcacca gatccgctcg aagcactctg ctcctggttc
      841 gagtccgtgt tgcgcggcgg ggtgtagcga agcttgcgca acaacgcccg atcgacgtcc
      901 tggtcggcca ggtaggccgt gagcagttcc ttgtaggtct gccgcaactc gcgccgctcc
      961 ccgtgcagac gcttcagttt cgcgacgtcg ctcatcccct tgcggaactt ttccgcatct
     1021 tccgccagtt ttttgagcgc ttcagagaac gacttcagtt cgagagcacc caggaatacc
     1081 gtatcgacca tgaaggattc gacgagaatt tccgggttaa gcttgtcatt ggcgacttcg
     1141 acgagaggaa tgtagttgaa gggagcctgt tcgatacgca agccgatgag cgactcgacg
     1201 ttaatacctg gctgaatctt gttcgaggcc agatacgact ggtacttttt cagcagcgcg
     1261 aaatcggggt tgaagtcaac atgccggatg ttcggattct tgcgaatctt ctcttcgaga
     1321 ctgtcttcga acacggtggt cttgatgcgg gcacccagtt caatcccttt ttcgatccag
     1381 gtcgaggggt tgatggcatc ggtgaacttg tcgaaccaac ccttgtcctt ctcttcctcg
     1441 acttcctgct gcgaaggctg ccacgacatg taggtcgctt tgtacttcca gagagccatc
     1501 gaggtttcga ggctgaagtc cttcatcttc cagggctgtt tgtaatagta tgaggtcaga
     1561 acgccctgct gctcgatctt ttcgagcaga ctttcgaagg ctttcttctc ttcgccttcc
     1621 atcttctcga cttcgagctt cttgcgcatc gcgatcgcct tcaggagcgc gagcgacatc
     1681 tcatcctggt gcaacagatc cgggttcttc cagttgtcga tcaacttctg aaagtccttg
     1741 tccttctgtt tcaaaaacca gaatgaatcc tggtaagcgg catgccattt cttcgcgtac
     1801 gcgccaacgg catccccgat catgcagaac acttcccacg ccgcctcgat cgcggcaacc
     1861 gcccacccaa tgggcgacag ggccgcaaaa cacagcatca gcacgaagac cgcggccaac
     1921 gcgatatcaa cgtagttttt cgtcatctca tacgagaacc agttatgcgc gccgcccatg
     1981 cgtcgttcgt tggaattagc gatgctgatg cccgccaaca ccccaccaag aatctgcaga
     2041 ccgacgccga ccgtcttgcc gatcccgacc gggttcgaac cgcgcgacac gaagttcttg
     2101 ccggcatcct ggttgaactt ggccgcccag aacttcttca gatattttcc ggtttcgccc
     2161 gcattttcgt agtcgacgct ggccacgttc tccatgaact tgagcacttt gttcgacacg
     2221 attttgtcga gcatcttctt gatctgcttc gagcgtttgg ccatgaactg catcgagtcg
     2281 ccgatcacct tgcccaggtt gcgacccttg cggccgtaga agcggccaat gttgtgcgag
     2341 aacagctcca gacgctgccc ccactttttg atcatggccg gaacacccgc caggccttca
     2401 aaaacatcct tgccaccgcc gaccgcgccg gccacccctt cgctctcttc tggcgaccag
     2461 ttcagcgtgt agtagcacag gagatcgggt gtttcgagaa cccccatgat cccggccagc
     2521 atctgcgcaa agtccgagac ggagttggct tcgatgacgg cctgatcgca gtctccctcg
     2581 aggccaccct tggccgcgag caactgcagc agcatctctt tcggcagcat gatatcgtcg
     2641 gcggtcacgc ggccgagata gtcctcgaca tcctgtttct cgatgccatt ctcgatcacc
     2701 tcgcgcgaga tcggtcgctg cggggtattc tccgcccgca cgacctcgat gaccgtcggc
     2761 tcgatcaaca gcagcagggc caggatcccc atcagaaccc gatgaaacct tctgattcgc
     2821 atactcaata cctcctgtgc gaatactccc tcgccggcga accggcgcac gatcagttac
     2881 cgttcaactt cgagcgggcc agtcgcgcaa tgaacgtatc gcgcccgttg tcccactgga
     2941 cgatctgttc aaacatcttc ttcgcctcag cctgattccc ccgagccgct tgagcctcgg
     3001 caaagtccat ggcaataccc agataccggg tgtcgggaac cccgctcggt ggggtcgaca
     3061 gttcggtctg gctgatttcc agcagcttgt cgttattgcc ggtctctttg taggcaagca
     3121 gcaggcggtc gtgcacctcg ctcttcagct tggtgttcgg atacttctgc aggaacgctt
     3181 cgagggtttc gatgatcctc gtccagtcgg gctcgctgta tccgctcatg tagagactgg
     3241 acaagcgcca gtaggattct tccgcccagt gcgtgtccgg gcagtcgttg atgacctgct
     3301 gatgcaatct gataaagtcg tccaacgccc agcgatccag ggccgccatt tctttgtaca
     3361 agtctttcgc caactttttc tgctcttcga gcgactttgt cgactttgcc tgtggctcaa
     3421 caggcgcagc gaccgcgaca tctggcttcg attgcggggc aggagagact ccccgtcctt
     3481 tgtcgatctg cgccttcagt tctttggcga accggttctc cggatctttt ccgagcacca
     3541 cctcaagttc cttcagtgat tcggtcttct ggccgagacg aaagcgaatc atcgccatct
     3601 gattgcgcag tgagagactt tccgggaact gctgcagggc ctgttccagg agttgtcggg
     3661 cttcgtcata gcgctgacgc cgacccgcga cggccacggc tttcttgtac aactcttcgg
     3721 atggcttgtc tttgatcagt tctcgatact gaaccatagc ctcttcggtc ttcccggctt
     3781 tttccagtgc tgtcgcctca tcgagtgaag cggcagaacc cacggcgggc ggcagccaga
     3841 gcgcgccgca gagaataatg ccgatcccca tcctgctcag aaatcgtcgc tgcatgcttt
     3901 acgacccctt tcgaaacgga ttttcccgcg ccagacagtg cgggaaaaac tgactggtaa
     3961 acgagagacg tgtccccttg gtgccttctc ccggttcttt cgagcgaatc ttgatcaaca
     4021 gatgaatgaa acgcaccgc
//
LOCUS       DAORHD010000300         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24128_length_4038_cov_4.287974, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000300 DAORHD010000000
VERSION     DAORHD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24128_length_4038_cov_4.287974"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKO06_07630"
     CDS             <1..371
                     /locus_tag="PKO06_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69548.1"
                     /translation="TFPALRRFLEKNRIPLAVAHPVIPIPGTPLYDRLKSENRLFDVT
                     PDLCDGLHVLYQPKHFTPEALTEAYWKFAAGLLSWRSIVRRFLWPGVLKNPLAWLVLL
                     LSNLFSRDVVRRRLPPGMYQ"
     gene            368..2911
                     /locus_tag="PKO06_07635"
     CDS             368..2911
                     /locus_tag="PKO06_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV69549.1"
                     /translation="MNRLSAFIDPRTIPAPISQDQVQMLGSKAANLDALRRAGFRTPE
                     FEILSADWFRRFVHGQLLTIPAALSDQEAQPIIDRILTAPLPAELLDELGRIRKRLGD
                     KPMCVRSSGTCEDLAGMSFAGQYESFLNLQNDEAIARAVRECWASLFNPRVRGYLKRH
                     GISLQHAGMALLVQEYVPAEYAGVAFTANPLTGRQNHLLLEYCAGGADKLVAGRITPS
                     QAVFDFESGHLLSGSLPPGMASHHLTEFRQIQKFFGAPQDIEWIWHNGSLCFVQTRPI
                     TSIKTEASFGTWTTADFRDGGVSSSVATPFMWSLYDYIWQFAMPGYCRILGLLDPGAD
                     QRPWGGMFFGRPYWNIGEVLTCLLKIPGFHERNLFSDLGIQTEAGYPFRSVPYSIEKV
                     TGVLPTLLKLEHYYRFRIQDNRRYRREFPELIKPFTEGRLERLSAADFAGRFRELITR
                     LYFRTETSYFMTIYNTSNAKLDFKVRLDAVNERGHDLSYIKLISGLLRVKHLAPLKSM
                     SHLAQQIAGQARIRENILKTPTDQIDSSLRNDPEGTQLLELFDQFIDLYSYHSSRELD
                     ISFPRWHEDRHTVWKLLKTYLREPDPESAISHERRQHEMYRQEVARARQAFGDLRHRL
                     LPFERWLFFRALLRTRTYCWWREEMRDYSSRVYAIIRTWALEAGRRLRLADGEIFWLT
                     WQQTLAALEGRMNGEQVRDLLSQAVEQALLYRHFENPDELGAGFLAKIPPETTGDASG
                     GTRLSGVGCSPGVAVGTARLIRSLEDSDRLQRGEILVTRFTDPGWTPIFHLLGGVVTE
                     TGGVLSHAAVISREYGIPAILNLTGATQRIRDGQRLRIDGSSGIVEVLP"
     gene            2908..>4038
                     /locus_tag="PKO06_07640"
     CDS             2908..>4038
                     /locus_tag="PKO06_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV69550.1"
                     /translation="MIHPSLHEISAHHDERPDEASERYTQFCYPGFGRQMHAFGLDVS
                     YIRADGDRLWYVDRQGVECEVLDLVGGYGVCLFGHNPGDLIATAQETLRQHPANMAQA
                     SVRSGAAEVGQRLSELAEERWHEPFCTALFNTGSDAIEAALKHCRLIRQAQIDQRLDQ
                     ILLSASQLIRRHQPRSISIDTRHAPRFPGTGFDSSITFLQALDSYNRVITSAPPTLLA
                     LRHAYHGLTPGALSLTDCPTFRRPFADLTLPTRFLEPTAEALANTLAGTQRMVARLEY
                     HHGTWRLVPEAISGVLAFFIEPVQGEAGIVEVCGTHVSEMVGLCRRNGIPVVADEVQS
                     GMGRTGRLFAWEHTGAPSPDLIVLGKALGGGLAKVAAVMSRQA"
BASE COUNT          764 a         1253 c         1144 g          877 t
ORIGIN      
        1 ccacgtttcc ggccttgcgt cgttttctcg aaaagaatcg cattccgctc gccgtcgctc
       61 atccggtcat tcccattccc ggtaccccgc tgtatgaccg gttgaaatct gagaaccggt
      121 tgtttgatgt gaccccggat ctgtgcgatg gtctgcatgt gttgtatcag ccaaagcact
      181 tcacgcccga agcgctgacc gaggcttact ggaaattcgc ggccggattg ctcagctggc
      241 gttcgatcgt gcgtcggttt ctctggcctg gcgttctgaa gaatcctctg gcctggctgg
      301 tactgctgtt atcgaatctg ttctcacggg atgtggtcag gcgtcgcctg ccaccaggga
      361 tgtatcaatg aatcggcttt ccgccttcat cgacccgcgc acgattccag ctcccatctc
      421 tcaggatcag gttcagatgt tgggaagcaa ggcagccaac ctggacgctc ttcgtcgagc
      481 ggggttcaga accccggagt tcgaaattct ttcggccgac tggttcagac gattcgttca
      541 cgggcaactc ctgacaatac ctgccgccct ttccgatcag gaagcacaac ccatcatcga
      601 tcgtattctg acagctccgc ttcccgcgga gcttctcgac gaactcggac gcatcaggaa
      661 acgcctgggt gacaaaccga tgtgcgtccg atcttccggc acctgcgaag atctcgccgg
      721 catgtctttc gccggtcagt atgagtcctt cctcaatctg caaaacgacg aggcgatcgc
      781 gcgtgcggtg cgagaatgct gggcttcgct gttcaacccc cgcgtgcgcg ggtatctgaa
      841 gcgacatggc atctcactgc aacacgccgg catggcgctt ctggttcagg agtatgtgcc
      901 ggcggagtat gccggcgtcg cgttcaccgc gaatccgctg accggtcggc aaaatcatct
      961 gctgctggaa tactgcgccg gtggcgcgga caagctggtg gcgggtcgta tcacgccgtc
     1021 gcaagcggtc ttcgacttcg agtccggcca tctcctctcg ggatcactgc caccaggcat
     1081 ggcgtcacac catctgaccg agtttcggca gattcaaaag ttcttcggcg caccccagga
     1141 tatcgaatgg atctggcaca acggctcgct ctgttttgtc caaacccgtc cgatcaccag
     1201 catcaagacg gaagcctcgt tcggcacctg gacaaccgcc gacttccgcg acggcggcgt
     1261 ttcgagttcg gttgccacgc cgttcatgtg gtcactgtac gactacatct ggcagtttgc
     1321 catgcccggc tactgtcgca ttctgggtct gctcgatccc ggagctgatc agcgcccctg
     1381 gggcggaatg ttctttggtc gaccgtactg gaacatcggc gaggtcctga cctgcctgct
     1441 caagatcccg ggttttcacg aacgcaacct gttttctgat ctcggcattc agaccgaagc
     1501 agggtacccg ttccgcagcg ttccgtacag cattgaaaag gtcaccggag tcttgccgac
     1561 tctcctgaag ctcgaacatt actatcggtt tcgaatccag gacaaccggc ggtaccgtcg
     1621 cgagttccca gaactgatca aacctttcac cgaaggtcgc ctggaacggc tttcagccgc
     1681 tgacttcgcc gggcgtttcc gtgagctgat cacccgcctc tattttcgta ccgagacgtc
     1741 gtatttcatg acgatctaca acaccagcaa cgccaagctc gatttcaagg tgcgactcga
     1801 cgcggtgaac gagcgtggtc acgatctttc ctacatcaag ctcatctcgg gtctgctgcg
     1861 agtgaaacac cttgcccccc tgaagtcgat gagtcatctg gcacaacaaa tcgctggtca
     1921 ggcgcgcata cgagagaaca ttctcaagac tccgaccgac cagatcgact ccagcctccg
     1981 caacgaccca gaaggaacgc agctgctcga gctctttgat cagtttatcg acttgtactc
     2041 ctatcattcc tcccgcgaac tcgatatttc gtttccccgc tggcacgaag atcggcacac
     2101 ggtatggaag ctgctcaaga cgtatcttcg cgagcccgac ccggaatcgg caatctcgca
     2161 cgaaagacgg cagcacgaga tgtatcgtca ggaagtggcg cgcgcccgac aggcgttcgg
     2221 tgacctgcga caccgcctgc tgccgttcga acggtggctg ttctttcgcg cattgcttcg
     2281 tacccgcacc tattgctggt ggcgtgaaga gatgcgcgac tactcgtcgc gcgtctacgc
     2341 gatcatccgc acctgggcac tcgaggccgg gcggcgactg cggctcgccg acggcgagat
     2401 cttctggttg acctggcagc agaccctggc agcactcgaa ggacgcatga acggagaaca
     2461 ggtcagagat ctgttgtctc aggcagtcga gcaggcgctg ctctaccgac atttcgagaa
     2521 tcccgacgag ctgggcgcgg gattcctcgc caagattcct cccgaaacga caggtgacgc
     2581 cagcggaggc acccgcctca gcggagtcgg ctgctctcca ggagtcgctg tcggaaccgc
     2641 tcgactgatt cgctctctgg aagacagcga tcgtctccag cgcggcgaga ttctggtgac
     2701 acggttcacc gacccgggct ggacgccgat tttccattta ctcggcgggg tcgtgacgga
     2761 gacgggaggt gttctgtcgc acgcggcggt catctcgcgc gaatacggca ttcccgccat
     2821 tctcaacctc actggtgcga cacagcgcat tcgcgatggc cagcgtttgc gcattgatgg
     2881 gtcctcgggt atagtcgaag ttctgccatg atccacccca gcctgcacga gatctctgcg
     2941 caccacgacg agcggcccga cgaagcttct gagcgttaca ctcagttctg ctacccagga
     3001 ttcggtcgtc agatgcacgc tttcggcctt gatgtctcgt acattcgcgc tgatggtgat
     3061 cgtctctggt atgtggaccg ccagggtgtg gagtgcgaag ttctcgacct ggtgggaggg
     3121 tatggcgtct gtctgttcgg gcataatccc ggagatctga tcgcaacggc tcaggagacg
     3181 ctgcggcagc accccgccaa catggctcag gcaagcgttc gcagtggtgc ggccgaggtc
     3241 ggtcagcgtc tgtccgagtt ggcagaagag cgctggcacg agcccttctg cacggccctg
     3301 ttcaacacgg gttccgatgc gatcgaagcg gctctcaaac attgtcgcct gattcgacag
     3361 gcccagatcg atcaacggct cgatcagatt ctgctgtcag cctcccagct gatccgcagg
     3421 catcagccac gatccatttc gatcgatacc cgtcacgcac ccagattccc cggcacaggc
     3481 ttcgattctt ccatcacgtt tttgcaggca ctcgactcgt acaatcgggt aatcacatct
     3541 gcaccgccaa cacttctggc gctgcgtcat gcctatcacg gtctgacacc cggcgctctc
     3601 agcctgacag actgtcctac gttccgccga ccgttcgctg acctgacgtt gccgacacgc
     3661 tttctggaac caaccgccga ggccctggcg aacactctgg ccggcaccca gcggatggtc
     3721 gcccgcctgg agtatcatca cggaacgtgg cgcctggttc cggaagccat cagcggagtc
     3781 ttggcattct ttattgaacc ggtgcaaggg gaagccggca tcgtcgaagt ctgtggcacg
     3841 catgtgtctg agatggttgg tctgtgccga cgaaacggca ttcccgtggt cgctgatgaa
     3901 gtacagagcg gcatgggacg tacaggtcgt ctgttcgcct gggagcatac gggtgcgccc
     3961 tctcccgatc tgatcgtgct cggaaaagct ctgggcggcg gtctggccaa agtggccgcc
     4021 gtcatgagtc gacaggcc
//
LOCUS       DAORHD010000301         4038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24129_length_4038_cov_3.952046, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000301 DAORHD010000000
VERSION     DAORHD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24129_length_4038_cov_3.952046"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKO06_07645"
     CDS             <1..724
                     /locus_tag="PKO06_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69551.1"
                     /translation="PLTIPLLELSGRLMLAEGKLGLRDSDCRLLGAPASLSLDIGLHS
                     WEKPLWNLQLAVPALDLGTVTADLPWVMRYSMGITPISGLASLTVHIGGEGPEMMCAA
                     QAPELRINLPAGDCRWTGKCEYRRSPVGGDRVTAEWSWLASETLRPVPRFLDRQAGGL
                     FERPQPPYAGTGRLTGTGHGAFFVGMYTAGSQRRDLRGTLADGRWNRLEVGIADADGA
                     LQSSSLLDPGPPLLALALLAGW"
     gene            complement(658..2226)
                     /locus_tag="PKO06_07650"
     CDS             complement(658..2226)
                     /locus_tag="PKO06_07650"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNV69552.1"
                     /translation="MTEQESGKPLEPKSAPEQQPLAALVDLKISPQLPGVVIQVHGNA
                     ESTQAYLSVHEIMEDVSCDEIETRVRDFLRNAVVKGHIDRFWNEEFPALIRKRKPFSQ
                     RLFAETSSPTEPVDTRIVHLVPRPTREFLIRPNGTCDFRNRDLFRSVSDGDGILVRYP
                     PQPGEPGMTIMGTQILPRPPKQVQVLAGKNVAVVDQEDGSQLYKATSTGQVVVENRGL
                     NFAVQVVPVLEIKSNVDYGCGNINFKGCVNIHGSVLSGFSVTATGNVTVCGMIEPKAR
                     VTAGGDLHVHKGILGNGTTDESTKVKAFGNVQALYAENAFIEAGGDVLLRSAMRCTIN
                     ANGGLFVEKVLVGGQISTFKSIEAAEIGNPTAMSTIIHCGSSPEALHRLNLLTRIIQD
                     LRKQKDKVDTNLRFVVTQGEDLPDDKRGPLQASLEKKAASLQDQIDRIEIKKADLAMN
                     LLDETASTISAQTLFPGVVLVIRSAKYVVEREMTSLTFYQKLPDEFLQFKTYQPAKSA
                     KASKGGPGSSKDED"
     gene            complement(2234..>4038)
                     /locus_tag="PKO06_07655"
     CDS             complement(2234..>4038)
                     /locus_tag="PKO06_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV69553.1"
                     /translation="PLELGTLREAPIKVYQGRTPIGIDYRLSRDLKTITLVPRTALKP
                     NQEYSVAITTQLAGASGSRLPKTTLIPFRVADQSAATDYEVPQHVLEEAPPEPGDTSF
                     DAAPSSALPSRRAAPVAAVPATPLKIVAMTPRQGETITNLSQPISIVFNQDVRPETLN
                     EFTFRVEDDFGPIPAKIRYIPERRQGVLTPVGVLDLQKTYRVIVTQGISDNAGGRLAK
                     GISASFSTQSPVNTPSTPEMFASAETAPTPVRQAKPRAPSSGRGLVPESFPDTAVRVP
                     ARSPSREVSHTDPRRESRELEVIDDESDQMSVSADMEEVVDEQPRSAPARKTSQRKSR
                     EALSTFKITGISPSANAENVKRDGRITLQFSDPANPSTLDEVNISVFANQRRVEGRVS
                     YDSRQQRAVFIPSEPFDAETNYKVRISDKIRSAIGEPLMGKYSWEFTTGTERRPSYVP
                     RGVMEADAAFSIPLADRRTVATRPAAEPGTGASSSFAYISPKHWAFRSIKHMHSKGLL
                     VTYPFTNKDRVTRYECAMSVNTILSTLKSLQNRPTPTQLRVADLVELEHLVIEFRPEL
                     KSLRTNPAWFEQFLQNQGVNLAEVQARVAQRNRG"
BASE COUNT          746 a         1129 c         1267 g          896 t
ORIGIN      
        1 accgctgacc attccgcttc tggaactatc agggcgactg atgctggcag agggaaaact
       61 gggtttgcgt gactcggact gtcgcctgct gggtgccccc gcgagtctca gtctcgacat
      121 cggtctgcat tcctgggaaa agccgctctg gaatctgcag cttgccgtcc ccgccctgga
      181 tctgggaacg gtgaccgctg atctgccctg ggtgatgcgc tattccatgg gaataacccc
      241 catctcgggg ttggccagcc tgacagtgca cattgggggc gaaggcccgg aaatgatgtg
      301 cgcggcacag gcccccgagc ttcgcattaa ccttcctgct ggcgattgcc gttggacggg
      361 caaatgcgag tatcgacgca gcccggtcgg tggagaccga gtgactgcgg aatggtcgtg
      421 gctggcttca gagacgttgc gaccagtgcc gcgattcctc gatcggcagg ccggtgggtt
      481 gttcgagcgc cctcaaccgc cgtatgccgg cacggggcgt ctcaccggca caggtcacgg
      541 agccttcttt gtcgggatgt ataccgcggg gagccaacgc cgggatttac gtggaactct
      601 ggcggacggt cgctggaatc gtctcgaagt cggcatcgcg gatgccgacg gagcgcttca
      661 atcctcgtct ttgctggatc ccggcccgcc cttactggct ttggcgctct tggcaggctg
      721 gtaagtcttg aactgcagaa attcatcagg cagcttctgg tagaacgtca gactggtcat
      781 ttcgcgttcg acgacatact tggcggaccg gatcaccaga accactcccg gaaagagggt
      841 ttgggcagaa atggtcgacg ccgtttcatc gagcagattc atggcgaggt ccgctttctt
      901 gatctcaatg cgatcgatct gatcctgcag gctggcggcc ttcttttcca gacttgcctg
      961 cagagggccg cgcttgtcat ctggaagatc ttcgccctgt gtcacgacaa atcgcaggtt
     1021 ggtgtcaacc ttgtctttct gtttgcgaag atcctggatg atgcgggtca gcagattcag
     1081 ccgatggagc gcttcgggcg acgaaccgca gtgaatgatg gtgctcatgg ccgtcgggtt
     1141 gccgatttcc gcggcttcaa tggacttgaa cgtcgagatc tgaccaccga cgagtacttt
     1201 ctcgacgaac aggccgccgt tggcgttgat cgtgcaccgc atggccgatc gtaacaacac
     1261 atcgccgccg gcctcgatga acgcgttttc cgcatagagc gcctggacat ttccgaatgc
     1321 cttgaccttc gtgctttcat cggtcgtgcc gtttccgaga atgcctttgt gcacgtgcag
     1381 atcaccgcct gccgtcacgc gtgccttcgg ctcgatcatg ccgcagaccg ttacgttgcc
     1441 ggtggcagtg accgaaaagc ctgaaagtac cgacccatga atgttgacac accctttgaa
     1501 attgatgttc ccgcacccgt aatcgacatt gcttttgatt tcgaggacgg gcacgacttg
     1561 tacggcaaag ttcaggccac gattttcgac gaccacctga ccggtcgagg ttgccttgta
     1621 caattggctg ccatcttcct ggtcgacgac tgccacgttt ttgccggcca gcacctgaac
     1681 ctgcttcggg gggcggggta gaatctgcgt gcccatgatc gtcatgcctg gttcgcctgg
     1741 ttgtggcgga taccggacca gaattccatc tccatcactc acactgcgaa agagatctcg
     1801 attgcgaaaa tcgcaggtac catttggacg aatcagaaac tcgcgagtag ggcgcggaac
     1861 aaggtggacg atccgggtgt cgaccggttc ggtcggagaa ctggtttccg cgaacaaacg
     1921 ttgcgaaaag ggtttccgct tacggatgag ggcaggaaac tcctcgttcc agaaacggtc
     1981 gatgtgtcct ttgaccactg cgtttcgcag gaaatcccgc acccgggtct cgatctcgtc
     2041 gcacgagacg tcttccatga tctcgtgcac agaaagatac gcctgggtgc tttcggcgtt
     2101 cccatgcacc tgaatgacaa cccccggcag ctggggcgaa attttcaagt cgaccagagc
     2161 tgccaggggt tgctgttcag gtgcggattt cggttcgagt ggcttcccgg actcctgttc
     2221 cgtcatgccg tgttcaacct cggtttctct gggcaacgcg tgcctgtact tctgccagat
     2281 tgacgccttg attctgcagg aactgctcga accaggccgg gttggtgcgc aggctcttca
     2341 gttccggtcg gaactcgatg accagatgtt ccagttccac gagatcggcg acccgcagct
     2401 gggtcggcgt gggccggttc tgcagagact tcagcgttga gaggatcgtg ttgaccgaca
     2461 tggcacactc ataccgggtc acccgatcct tgttcgtgaa cgggtaggtg accagcagac
     2521 ctttggagtg catgtgcttg atcgatctga aagcccagtg cttgggcgaa atgtaggcga
     2581 aggaactgct ggcaccggtt cccggttctg ccgccggacg ggtagcgacg gtgcgccggt
     2641 ctgccagcgg aatcgagaat gccgcatcgg cttccatgac gccgcgaggc acgtagctcg
     2701 gccgtcgttc cgtcccagtt gtgaattccc aggagtattt acccatgaga ggttcaccaa
     2761 tggcagaacg gattttgtcg ctgatgcgta ccttgtagtt cgtctcggca tcgaacggtt
     2821 cgctgggaat gaagaccgcg cgctgctgtc gggagtcata ggaaacccgg ccttcgacgc
     2881 ggcgctgatt ggcgaacacg gagatgttga cttcatccag cgtcgaggga ttggcgggat
     2941 cgctgaactg cagcgtgatt cgaccatcgc gcttgacgtt ctcggcattg gctgaggggc
     3001 tgatgccggt gatcttgaag gtcgacaggg cctcgcgtga cttgcgctgg ctggttttgc
     3061 gggccggggc gctccgcggt tgttcgtcga cgacttcttc catatcggct gaaacgctca
     3121 tctgatccga ttcgtcgtcg atgacttcca gttcgcggga ctcgcggcgc gggtcagtat
     3181 gactgacttc gcgagagggc gaacgggccg gaacgcgaac cgccgtgtcg gggaacgatt
     3241 cgggtaccaa cccgcgccca gaggaaggag ctcggggctt cgcttggcga acaggggttg
     3301 gagcagtttc ggcagaagca aacatctcag gagtcgacgg agtgttcacc ggtgactggg
     3361 tggagaacga agcgctgata cctttggcca ggcgtccacc ggcgttgtca ctgataccct
     3421 gggtcacgat gacccggtag gttttctgca gatcgagcac tccgaccggg gtcagaaccc
     3481 cttgtcggcg ttcgggaatg tagcgaatct ttgccggaat agggccgaag tcgtcttcca
     3541 cccggaaggt gaactcattc aacgtttccg gtcggacatc ctgattgaac acgatcgaaa
     3601 tcggctggct caggttggtg atggtttcac cctgacgcgg ggtcatggcc acgatcttca
     3661 gcggcgtggc gggcacggct gcaacgggcg cggcccgtcg agacggtagt gcggatgagg
     3721 gagccgcatc gaaagaggtg tcacccggct cgggcggcgc ttcttcgagg acatgctggg
     3781 gaacttcata atccgtcgcg gcgctttgat cagccacgcg aaagggaatc agcgtggtct
     3841 tcggcagccg cgacccggaa gctcccgcga gctgggtggt gatggcaacg ctgtattcct
     3901 gattcggctt cagcgcggtg cgtggcacca gggtaatcgt cttgagatcg cgactcaggc
     3961 gatagtcgat accgatcggt gtgcgtccct gatacacctt gatcggagct tcgcgcagtg
     4021 tgcccagttc gagaggct
//
LOCUS       DAORHD010000302         4031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24201_length_4031_cov_3.657948, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000302 DAORHD010000000
VERSION     DAORHD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24201_length_4031_cov_3.657948"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..626
                     /locus_tag="PKO06_07660"
     CDS             147..626
                     /locus_tag="PKO06_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69554.1"
                     /translation="MLKRLFLGLPLVIGLFVGIIAAPVWAEEEAAKPDEATLERNAEE
                     VLFATSKEQGAPKGAVTLANTFRRYLLAGNNKKVLALFAEEDQDYYHDRIMLMKKKDL
                     QTQGLYFRKAKCASATDKSAVYNYRPNTKYKIGNLYMKFDGKKWWCTDWFQSTKYAK"
     gene            complement(714..1439)
                     /locus_tag="PKO06_07665"
     CDS             complement(714..1439)
                     /locus_tag="PKO06_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69555.1"
                     /translation="MIEFRGLTKRYGQRTAVDSLKFSVPDGALFGFLGPNGAGKTTTI
                     RMLMGLLHPSAGQAFIDNIDVTRDSLEVRRRVGHLPDEIFLYDYLTGRQFLEFIGDIH
                     RLERSVRDRRISEYLEFFQLDHAADEFTTNYSFGMKKKLALAGIVLHEPKVLLLDEPF
                     NGLDPQAVKDLRQLLEKMRRTGATILFSSHVLEVVEKLVTHVGIIASGRLRSAGTLEE
                     VTAAHGDSLEKAYFAFTEQSDAG"
     gene            1556..2446
                     /gene="larE"
                     /locus_tag="PKO06_07670"
     CDS             1556..2446
                     /gene="larE"
                     /locus_tag="PKO06_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNV69556.1"
                     /translation="MADKKQVCSQIPPLMACTTAPSAPLSVEAVQALDKLEQRLSLLN
                     GLVVAYSGGMDSGFLALTARRILGTRMKAVFLQTAFIAEADGERARALAHQFNLPLTI
                     LTADVLAEPALRANPDNRCYLCKQLVIKHLRTEVPPGWELGEGSVTDDAGDYRPGKQA
                     LREAGVLSPLDAAGFSKSLVRECLMAMNAGEFVRPSQSCLATRIPAGQAVNEERLRRI
                     DRGEAFLAGLGLSNLRLRDHDGLVRLECPPEQWSLIIEHRAEILQNLQSLGFTHITLD
                     LGGYRRGSTNRIQPAGNPHP"
     gene            2443..3186
                     /gene="larB"
                     /locus_tag="PKO06_07675"
     CDS             2443..3186
                     /gene="larB"
                     /locus_tag="PKO06_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNV69557.1"
                     /translation="MKKLLQDVAAGRTSVRQAERALEERFSRLPVGNDVCLDLGREHR
                     TGFPEAVFGAGKSVEQVVKAFFGLVDHQGKALATRCSAEALTALRQKNRRVKTHPASG
                     VAWYVKQPVKSRHLVAVAAAGTSDLRVAEEAALTLEFAGIGVERVYDVGVAGIHRLLG
                     KLPVFHRADAAIVVAGMEGALPSVLGGLTDIPLIAVPTSVGYGAGLQGLSALLTMLNS
                     CAPGLAVVNIDNGFGAAVAVIRILKGTHT"
     gene            3183..>4031
                     /locus_tag="PKO06_07680"
     CDS             3183..>4031
                     /locus_tag="PKO06_07680"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HNV69558.1"
                     /translation="MSTLYWDPFSGIAGNMAVGSLLDLGADPERLSRQLASIPFPEGR
                     LQLVVERQVKKGFDGIYFNTVDDDGPREHSHGHGHENGHPHDHEHDHEHDHEHDHHHG
                     HDHEHEHDHGHDHRHEHNHDHDHGHDHEHDHGHNHEDRHHHTHGPDHEQSDVSRRHHD
                     HSHHGPDHAHPHASNTCAHRHAAKNQTHNPHVHRGMTEIRDLLQQADLAPNARDTALD
                     CFAALAEAEGRVHGKPADAVHFHEVGARDSIADLVGVAICLHDLGITSVATGPVHLGS
                     GFITCQH"
BASE COUNT          865 a         1198 c         1171 g          797 t
ORIGIN      
        1 cttccggaac ccacacgatt agcccatctc gaagcgaatg cggcccgcaa gaagggatgt
       61 cgacaataac ttgacagttc gccggcaatt cttagaatag aagtgacgat actattggta
      121 cacttttccg gcatgaggag gaaattatgc tgaaacgttt attcctgggc ctgccacttg
      181 ttatcgggtt gtttgtcggc atcatcgccg cgccggtttg ggctgaagaa gaagccgcca
      241 agcctgatga agcgactctt gaacgcaacg cggaagaagt tctgtttgcc acttccaagg
      301 aacagggagc cccgaagggg gcggtaactc tcgccaacac cttccgacgt tatctgctcg
      361 cgggcaataa caagaaagtg ctcgccctgt tcgccgaaga agaccaggat tactatcatg
      421 atcgaatcat gctgatgaag aaaaaagatc ttcagacgca gggtctgtat ttccgcaagg
      481 ccaaatgcgc cagcgccacg gacaagagtg ccgtgtataa ctatcgcccg aataccaagt
      541 acaagatcgg aaacctctac atgaagtttg acggcaagaa gtggtggtgc acggactggt
      601 tccagagcac gaagtacgcg aagtaaaacc gccctctcgc agcaatcgac cgagccttgg
      661 cgagcaatcg ccggggctcg tttgcgtttg aaccggttgt tcttggatgg aaatcagccc
      721 gcgtcagatt gctcggtgaa ggcaaaatag gctttttcca gcgagtcgcc gtgggctgcg
      781 gtcacttcct cgagggtgcc ggccgatctg agccggccgc tggcgataat gcccacatgg
      841 gtgaccagtt tctcaacgac ttcgagcaca tggctggaaa acaggatcgt cgccccggtt
      901 cgccgcattt tttcgagcaa ctggcgtaga tctttgaccg cctgcggatc aagaccattg
      961 aacggctcat ccagcagcag cactttgggt tcgtgcagaa cgatgcccgc gagagccagt
     1021 ttctttttca tgccgaaact gtagttggtc gtgaactcat cggccgcgtg gtcaagctga
     1081 aagaactcga gatactcgga aatgcggcgg tcgcgaaccg accgttcgag ccggtgaata
     1141 tcgccgatga attcaagaaa ctgccgaccg gtcaggtaat cgtacaagaa gatttcatcc
     1201 ggcaggtgcc cgacgcggcg cctcacttcg agagaatccc gggttacgtc gatgttatcg
     1261 atgaacgctt gtccggcact tgggtgcagt aaccccatga gcatgcgaat ggttgtcgtc
     1321 ttgccggcgc cattcggacc aagaaagccg aacaaggctc catccggaac cgaaaatttc
     1381 agactatcga cggccgttcg ctgtccgtag cgtttcgtga gacctcgaaa ttcgatcatg
     1441 caacaatcgc ccccgcgtga gcattacttt acccggtcat gaccggaata tccacccttt
     1501 tccgttcaga tttcgaggcg tgaagaccag ggaaaggtgt ggtatgatgc ggcgcatggc
     1561 tgacaagaag caggtttgct cgcagatccc tcccctgatg gcatgcacaa cggctccgag
     1621 tgcgccgctg tccgtcgaag ccgtgcaagc tctggacaaa ctggaacagc gcctctctct
     1681 tctgaacggc ctggtcgtgg cgtattcggg cggcatggac agtggctttc tggctctgac
     1741 ggcacgacga atcctcggaa cacgcatgaa agcggtgttc ctgcagaccg ccttcatcgc
     1801 cgaggctgac ggggagcggg cccgcgcgct cgcccaccag ttcaatctgc cgttgacgat
     1861 tctcaccgcc gacgtgctcg ctgagcctgc tctgcgcgca aaccccgata accgttgcta
     1921 cctctgcaag caactggtca tcaagcatct gcgcaccgag gttcccccgg gctgggaact
     1981 gggtgaaggc tcggtgactg acgatgcggg ggattatcgc cccggcaagc aggcactgcg
     2041 tgaagccgga gttctcagcc ctctggacgc agccggattc tcgaaatccc tggtgcgcga
     2101 gtgcctgatg gccatgaatg ccggagagtt cgtgcggcca tcgcagtcgt gcctcgccac
     2161 gcgcattccc gcagggcaag cggtcaacga agagcggctg cgccgaatcg atcgcgggga
     2221 agcatttctc gccggtcttg gattaagcaa cctgcggctg cgagatcacg acgggctggt
     2281 gcgtctggaa tgccccccgg agcagtggtc attgatcatc gaacaccggg ccgagattct
     2341 gcagaatctc cagagcctcg gattcaccca catcaccctc gacctgggcg gttatcgccg
     2401 cggatcgacg aatcggattc aacccgcagg aaacccacat ccatgaaaaa actgttgcag
     2461 gatgttgccg ccggtcgtac cagcgtccga caggcagaac gagcactgga agagcgcttc
     2521 agtagacttc ctgtcggcaa tgacgtgtgt cttgatctcg gtcgcgagca tcgtacaggt
     2581 tttcccgagg cagtgttcgg agcaggaaaa tcggttgagc aggtcgtcaa ggccttcttc
     2641 gggctagtcg accatcaggg caaggccctg gccacgcgct gttcggccga agctctgacc
     2701 gctctgcgcc aaaagaaccg gcgggtcaag actcatcctg catcgggggt tgcctggtat
     2761 gtgaagcaac ctgtcaagtc acggcatctg gtcgcggtcg ccgcggcagg gacctcggac
     2821 ctgcgcgtcg cggaggaagc ggctctgacg ctggaattcg ccggcatcgg cgtcgaacgg
     2881 gtctacgacg tgggcgtggc cggcattcat cgtctgctgg gaaagttgcc cgtctttcat
     2941 cgagccgacg ccgcgatcgt cgtggcgggc atggagggag ctctgcccag tgtcctgggg
     3001 ggacttaccg acattcccct gatcgccgtt cccacgagtg tcggctacgg cgccggactg
     3061 cagggcctga gcgctctgct gaccatgctc aactcgtgcg cccccggtct cgccgttgtc
     3121 aacatcgaca acggattcgg agccgcggtc gccgtcattc gcattctgaa aggcacacac
     3181 acatgagcac cttgtactgg gatccctttt ccggtattgc cggcaacatg gccgtcggct
     3241 cgctgctcga cctgggcgct gatcctgagc ggctttcccg acagttggcc tcgattccct
     3301 ttccggaagg tcgtctgcag ttggtcgtcg aacgacaggt gaagaagggc tttgatggca
     3361 tctacttcaa cacggtcgat gacgacggtc cccgcgagca ctcgcatggg cacggtcatg
     3421 aaaatggaca cccgcatgat cacgagcatg atcacgagca tgatcacgag catgatcacc
     3481 atcacggaca tgaccacgag cacgaacacg atcatgggca tgatcatcgg cacgagcaca
     3541 atcatgacca cgatcatggc catgatcacg agcacgacca tgggcacaac cacgaagaca
     3601 ggcatcatca cacgcacgga cctgatcatg agcaaagtga cgtttctcgc cggcaccatg
     3661 atcactcgca tcacgggccg gatcacgcgc acccacatgc gagcaatacg tgcgcccacc
     3721 gccacgcggc caaaaaccag acgcacaacc cgcacgtgca tcgcggcatg accgagattc
     3781 gcgacctgct gcagcaggcc gatcttgcgc cgaacgcgcg tgataccgct ctggactgtt
     3841 tcgcggccct cgccgaagcg gaaggacgcg tgcacggcaa gcccgccgat gccgttcatt
     3901 ttcatgaagt cggtgcgcgc gacagcattg ccgatctggt cggggtcgcc atctgtctgc
     3961 acgatctcgg cattacgtcg gtcgcaaccg gccctgtcca tcttggttcc ggattcatca
     4021 cctgtcagca c
//
LOCUS       DAORHD010000303         4023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24287_length_4023_cov_3.453125, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000303 DAORHD010000000
VERSION     DAORHD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24287_length_4023_cov_3.453125"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKO06_07685"
     CDS             <1..638
                     /locus_tag="PKO06_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69559.1"
                     /translation="MVQQSIESVLVNDQFRKVLFYMGKDVREASLILHPPMVRPEEVV
                     GLTTKTGVVLHLVRQEVDPSIKPSATGLPVARNQEIIYRLEPFLATNDDDSAPAQAEP
                     RFRLVRLERVKEAQANTVEQRMEVTDMVRDLVIYRTLRKPMINQNIEGADGFILDPVT
                     PRNNGTGYGVVHVRTILERDRRRTATIEREVYGVSLSTCFSMRGKAMWKNP"
     gene            635..2938
                     /locus_tag="PKO06_07690"
     CDS             635..2938
                     /locus_tag="PKO06_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69560.1"
                     /translation="MSTVNSRRGMAYFVVMAFAVIMAMFLMLLGRMNSGAIQLLSKNA
                     RDHLAAMVAEAGLNCIIAELHVNNDYTTHGMYKWGGDAPWAKPIKDRPSALKAVRDLE
                     TFGVKEGVYRGKLQDAGEFKVKLAGTFGKDNPLTKTLKENQMYLVAEVVAAVRAGGKS
                     EEQSYRRVKALIEKRSVASEHLLFDGEMLDMGLGPYVGAPNQLRRGRLYGYQWVTFNS
                     AGGPDKGSEMFEMEKIETAGMIKAGVKAPVEFADKTSTSITAGNDSTSGKFKTYDGYL
                     LDGNTGGRPIKMAELPKDSYLAKAKRRKNTGGLVIEAGKVPSTIPLSTWKNPYDEKTD
                     YYDLNFGGYVVGQDEPDADGDSQDEADDTNANDPPYGSDDPAPPGTLRGRALLIYSEV
                     PLRIWGCPDRSVTIFCEKDVVIAGDFNQNPDTMQDYPDTTYQTYKTPIQNGKRFHKVG
                     AMILSMGRVMIDHSRPSLFLYNEMRAYLSWLIAMALHPVDKETEEEARKALCPADPKK
                     RGGIIGLGAIDPATNRPKPIYNEIYFLKEQTSVATGPLYMSHMADLIELFTPGNDARP
                     RFGIKNEVNRLRVIQEIDQACRGDGVITRDDFDRIVNLAWEFAVEEEAKEPSADQGAM
                     GLARFLFDEAGKKPDDGLWPPEITVNAALVTSTRRHAKWTTGNAGPKVFDEIGNINVK
                     SKKLVEYLKPPKFLIQRVFGSEIRLGSRAETWYLSGDYTGQGVLRRRIWDKSLTGGKY
                     QPTALPFAFNIFSYSEEAISKKEYEAF"
     gene            2944..>4023
                     /locus_tag="PKO06_07695"
     CDS             2944..>4023
                     /locus_tag="PKO06_07695"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV69561.1"
                     /translation="MGQPQPHSFLQKPEVRCTLVFLLLCSVLSVTGIFDGIEVKTLDW
                     RFQQRPAASPDRRIVMLYVSDECLAELGKWPWSRELHARAVQELHCMGVAALVVDILF
                     QEPADREPESDLTLVEACRALGRAILPMYVIPVNIVHPETGRLSTTFEAMPPFTALAS
                     VSASMGSINVDYRHLNPDGGMRRPLLYSRIEERWYPSLALAAAQVVTGSPARLERQGL
                     FLGDRPVPLHEYPVLESVSWYWRLVSKPALLVNYQGEADSGAFPIVYVSDLLRGSVAP
                     DFFRDKIVLYGPSAIGLADIKLTPYGEMPGVMIHANILHNLINGAFLRQAEVPVQIAI
                     LALLAGAVLFTLLRAGPVSGLVILGG"
BASE COUNT          875 a         1080 c         1229 g          839 t
ORIGIN      
        1 gcatggttca acagtctatc gagtcggtgc tcgtcaacga tcagtttcgc aaagtcttgt
       61 tctatatggg gaaagacgtc cgcgaagcca gtctgatcct gcatcccccc atggtgcgcc
      121 cggaagaggt ggttggtctg accacgaaga ccggtgtggt tcttcatctg gtgcggcagg
      181 aggtcgatcc cagcatcaag ccgagcgcaa cgggccttcc ggtcgcccgc aatcaggaaa
      241 tcatctatcg ccttgaacct ttcctggcaa caaatgacga tgattccgct ccggcacagg
      301 cggaaccacg tttccgtctg gtacgcctcg aacgcgtgaa agaggcccag gctaacacgg
      361 ttgaacagcg catggaagtc acggatatgg tgcgtgatct cgtgatctat cgcacgttac
      421 gcaagcctat gatcaaccag aatatcgagg gcgctgacgg ttttatcctc gatccagtga
      481 cgccgcgaaa caatggcaca gggtatggag ttgtccatgt gcgcacgatt ctggaacgcg
      541 atcgccgtcg tacggcgacc attgagcgcg aggtctacgg cgtatcgctg tcgacctgtt
      601 tttcgatgcg cggaaaagca atgtggaaaa acccatgagc acagtgaatt cgcgacgggg
      661 catggcgtat ttcgtggtga tggctttcgc cgtcatcatg gcaatgtttc tgatgctgct
      721 gggtcgcatg aacagtggag cgattcagct gctctcgaaa aatgcccgcg accatctcgc
      781 cgccatggtg gcagaagcag ggttgaactg catcatcgct gagttgcatg tgaacaacga
      841 ttacaccacg catggaatgt acaagtgggg cggcgacgca ccgtgggcca agccgatcaa
      901 agatcgtccg agcgcgctca aggcggtgcg tgacctcgaa accttcgggg tcaaggaagg
      961 tgtgtatcga gggaaattgc aggatgcggg cgagttcaag gtaaagctgg ccggtacgtt
     1021 tggcaaagac aaccctctca ccaaaacgct gaaagagaat cagatgtatc tggtcgccga
     1081 agtcgtcgcc gcggttcgcg ccggcggcaa atcggaggaa cagtcgtatc ggcgtgtgaa
     1141 ggctttgatc gaaaaacgga gcgtggcgtc ggaacacctg ttgttcgacg gggagatgct
     1201 ggatatgggc ctgggtccgt atgtcggggc tcccaaccaa ttgcgtcgcg gtcgcctgta
     1261 tggctaccag tgggtgacgt tcaactccgc cggcggaccg gacaagggtt ccgagatgtt
     1321 cgaaatggaa aagatcgaaa cggcagggat gatcaaagcc ggggtcaagg cccccgtgga
     1381 gtttgccgac aagacctcga ccagcatcac tgcgggtaat gactcgacca gcggcaagtt
     1441 caagacctat gatggctacc tgcttgacgg gaacaccggt ggtcggccga tcaagatggc
     1501 ggaactcccc aaagacagtt atctggcgaa ggccaaacgc cgcaagaata ccgggggtct
     1561 ggtcatagaa gcaggaaaag tgccttcaac gattccgctg agcacctgga aaaaccccta
     1621 tgacgagaag acagactact acgatctgaa ctttggtggc tacgtggtgg ggcaggacga
     1681 accggacgcc gatggtgaca gccaggacga agcagacgac acgaatgcca acgatccgcc
     1741 ctatggcagt gacgacccgg ctccgccggg gacactgcgc ggtcgggcct tgctgatcta
     1801 ttccgaagtg cctttacgca tctggggctg cccggatcgc agcgtgacga ttttctgcga
     1861 gaaagatgtc gtgattgccg gggacttcaa tcagaatcct gacaccatgc aggattaccc
     1921 cgacaccacc taccagacct acaaaacccc catccagaac ggaaagcgtt ttcacaaggt
     1981 gggagccatg attctctcga tggggcgggt gatgatcgat cattcgcgac catcgctctt
     2041 cctgtacaat gaaatgcgtg cctatctgag ttggctcatc gccatggcct tgcatccggt
     2101 cgacaaggaa accgaggaag aggcacgaaa agcgctgtgc ccggctgatc cgaaaaagcg
     2161 tggtggcatt attgggctgg gtgccattga tccggcgacc aaccgaccga agccgattta
     2221 taacgagatc tactttctga aggaacagac gtcggtggcc acggggccgc tctacatgag
     2281 ccacatggcc gacctgatcg agttgttcac gccgggcaac gatgcgcgcc ctcgcttcgg
     2341 catcaagaat gaagtcaatc gcctgcgcgt cattcaggaa atcgaccagg catgccgcgg
     2401 cgacggtgtc attacccgcg atgatttcga tcgtatcgtc aatctcgcgt gggagttcgc
     2461 cgtcgaggaa gaagcgaaag agccttctgc tgatcaggga gccatggggc tggcgcgctt
     2521 tctcttcgat gaagccggga aaaagccgga tgacggactc tggccacccg agatcacggt
     2581 gaacgcggcg ctcgtcacgt cgactcgccg acacgcgaag tggacgacag gcaatgctgg
     2641 tccgaaggtt tttgacgaga tcggcaacat caatgtcaag agcaagaaac tggtcgagta
     2701 tctgaaaccg ccgaagtttc tgattcagcg tgtcttcggc tcggagattc gcctgggcag
     2761 ccgagcggaa acctggtatc tatcgggaga ctacaccggg cagggcgtgt tgcgccgaag
     2821 gatctgggac aaatcgctga ccggtggcaa gtatcagccc accgctctac ccttcgcttt
     2881 caatattttt tcctactcgg aagaggcgat ttcaaagaaa gagtacgaag ccttctgagc
     2941 tctatggggc agccacagcc gcattcgttt ctgcagaaac ctgaagttcg ctgtactttg
     3001 gtctttctgc tgctctgcag cgttctgagc gtgacgggaa tcttcgacgg aatcgaagtc
     3061 aaaacccttg actggcgatt tcaacagcga ccggctgcgt cgcctgatcg gcgcatcgtc
     3121 atgttgtatg tgtccgatga gtgcctggcc gaactgggca agtggccctg gtctcgagaa
     3181 ctgcatgcgc gggcggtgca ggagttgcac tgtatggggg ttgcagcgct ggtggtcgac
     3241 attctgtttc aggaaccggc ggatcgagag ccggaatctg atctgacgct ggtcgaggca
     3301 tgccgcgcgc tggggcgtgc gatcctgccc atgtatgtca ttccggtgaa catcgtccac
     3361 ccggaaaccg gtcgtctgtc caccacgttc gaagcgatgc cgccattcac ggctctggcc
     3421 tcggtttctg cctcgatggg ctcgatcaac gtggattacc gccatctgaa cccggatgga
     3481 ggcatgcggc gcccgctgtt gtacagccgc atcgaagaac gctggtatcc gagtctcgct
     3541 ctggcagccg ctcaggtcgt gaccggaagc ccggcgcgtc tggaacgaca aggcttgttt
     3601 ctcggtgacc gtcctgttcc actgcatgaa tacccggttc tcgagtcggt ctcctggtac
     3661 tggcgcctgg tctcgaaacc ggcattactg gtcaactatc agggagaagc tgattcggga
     3721 gcctttccga tcgtgtatgt ttccgatttg ctgcggggat ccgtggcacc cgattttttt
     3781 cgggacaaga tcgtattgta cggaccgagt gcgatcggtc tggccgacat caagttgacg
     3841 ccctacggcg aaatgccggg ggtgatgatt cacgcgaata ttctgcacaa tctgatcaat
     3901 ggcgcattcc tgcgtcaggc agaggttccg gtgcagatcg cgattctggc gctgctggca
     3961 ggcgcggtgt tgtttacctt gctgcgggcc ggaccggtga gcggtctggt gattctgggc
     4021 ggg
//
LOCUS       DAORHD010000304         4021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24301_length_4021_cov_3.725416, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000304 DAORHD010000000
VERSION     DAORHD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24301_length_4021_cov_3.725416"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /locus_tag="PKO06_07700"
     CDS             complement(<1..1650)
                     /locus_tag="PKO06_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69562.1"
                     /translation="MVPRFSFPLPRPGFVILMFATLFASLLSADDNTINLDGSRLKMV
                     VQARVRASGADQRATANEFARINLVFNTIENYLIYLEKLGRIATDTVLSMEYAPEGST
                     RDRPVDRWVVFDELLFNSVLSRVNTFLSERPGTRRNDLIQDRGALRNVRDQLPRRDDA
                     VENSYRWKNFSELMPAIEPDVDFAALQTNRVVGLRPTDLGYTLVRLDASESAGIELAS
                     FSESVWDLTPSTDGTWVACTVNGDLRLINTINRQMVNLFDPKTYQLLDMAWAPGQPLL
                     AGIVLERRTGEREIFLYDAAKAKKLSIPLKEQGLNGNYQFAWPCWSPDGTRVLFTSGN
                     DFSLIDLPSGKVHAGLFQTPNTITELVWAPDSRSFAFVEILGQSRNKGEFDDRDFRGS
                     ILHRFRIPTEGAPFEETSQRYLSEQTVKLISFWSQDRLFFLEGRLRQQKVHSPFWDLS
                     DVFAARLTPPPSPSTPSATMTAGITSGGLEIRLDHCYVFRNLDSRFRNVYDAGRASTN
                     WLFTDRMANVWFLGITPPEGFPRRVDTYSLRMQPYPFGERNT"
     gene            complement(1655..2767)
                     /gene="ispG"
                     /locus_tag="PKO06_07705"
                     /gene_synonym="gcpE"
     CDS             complement(1655..2767)
                     /gene="ispG"
                     /locus_tag="PKO06_07705"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNV69563.1"
                     /translation="MTHAPRRHSRAIRVGTVTVGGDAPVSVQTMTKTDTRQAEATITE
                     IRRLSLAGCEIIRLAVPDQAAAESLRTIVPAAPIPVIADIHFDHHLAILAVKAGVHGL
                     RINPGNLRDRQKVREVVAASRDRGIPIRIGVNAGSLDPAMAQRYGGVTAQALAESALQ
                     EVHLLEAEGFSNIKIAIKAFEVSLMVEAVRQVARACDYPLHLGVTESGLPDEGVIRSS
                     IGIGTLLLEGIGDTIRVSLTGDSADEIAAGIRILRVTGHRQVGPVIVSCPTCGRCQVP
                     LMRMAEAVRDRLSHLTQPVRIAVMGCAVNGPGEARQADFGIAGAKDGGLIFKHGQILR
                     HLPSDQLVEGLVQEISASLGIPIPPAKPRCSSDAKE"
     gene            complement(2764..>4021)
                     /locus_tag="PKO06_07710"
     CDS             complement(2764..>4021)
                     /locus_tag="PKO06_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69564.1"
                     /translation="AAAFNPLKSLVGMLVDYLFGRRNFDSAAVIRHLLREMRRAETAE
                     TVCANLLRESAPVLGFGQAVVLLADGVRVSWPFDTPSVSVSFSSIPTDLTEKAEEIDS
                     LLEIALESAAPCRPVLQAWQSAGFTLLYPIVREQSCVGLLLLSAKTSRLPYSEQERSL
                     IGSIVNEIGPVLDNLHLVASLLERDRAAREVELTLRMYRQIQATNGCHSVGNWRLFLF
                     SSLADTIKGDLIDLDPDSSPPFLCLCDAFHQGVPAALTLYIIRTALRSAPAEQRFLIL
                     HRLLRASSEPPLRAALTFAVFAADGLHFSCAGNPPPLLLAPGQPPRALVDPGSPLGLD
                     EAPRLTTTLVAPGTGAIVCLATNGLWKAFGDDSGDGLRAFLAAQSGCSAHDLYQRLSQ
                     HLTITQTSATIQDDISFVLIQENSIP"
BASE COUNT          876 a         1097 c         1287 g          761 t
ORIGIN      
        1 ggtattgcgt tcaccgaacg ggtacggttg cattcgcaga ctataggtgt cgacgcgacg
       61 gggaaagccc tcgggcggtg tgattccaag aaaccagacg ttggccatgc ggtcggtgaa
      121 cagccagttt gtactggctc tgcctgcgtc atacacgtta cggaagcggc tatcgagatt
      181 acgaaagacg tagcagtgat cgagacggat ttccagacca cccgaggtga tcccggcggt
      241 catggtggcg gagggagtgc tgggactggg aggcggagtc aggcgtgccg caaagacgtc
      301 ggagaggtcc cagaaggggc tgtgaacttt ctgttgcctg agtcgaccct ccagaaagaa
      361 caggcgatcc tggctccaga agctgatgag cttgactgtc tgctcggaca gatacctctg
      421 agaagtctct tcgaacggtg cgccttcggt cggaatcctg aaacgatgca gaatcgaacc
      481 ccggaaatcg cgatcgtcga actccccttt gttccgcgat tgtccgagaa tctccacgaa
      541 agcgaaggag cgactgtctg gtgcccagac cagttcggtg atggtgttcg gagtctgaaa
      601 cagcccggcg tggacttttc ccgagggcag atcgatcagc gagaagtcgt tccccgaggt
      661 aaagagaacg cgcgtgccat ctggcgacca gcaaggccac gcgaattgat aatttccatt
      721 cagaccctgc tcttttaatg gtatcgagag tttcttggct tttgcggcat cgtacaaaaa
      781 gatctcgcgt tcgccggtgc gacgttcgag aacgatcccg gcaagcaggg gctgaccggg
      841 tgcccaggcc atatcgagga gttgataggt tttggggtcg aaaaggttga ccatctgtcg
      901 attgatcgtg ttgatcagtc gcaaatctcc gttgacagta caagccaccc aggtgccatc
      961 tgtcgatgga gtcagatccc agacgctttc agaaaacgaa gccaattcga tgcccgcgct
     1021 ttcggaagcg tccaggcgaa ccagggtgta tccgaggtcc gtcggtcgta acccgaccac
     1081 gcgatttgtc tgcagggctg cgaaatccac atccggctcg attgccggca tgagttccga
     1141 gaagttcttc catcggtagc tgttctcgac ggcgtcatcg cgccgtggca gttgatcgcg
     1201 aacattgcgc aaggccccgc gatcctgaat cagatcattg cgtcgggtgc cgggtctctc
     1261 gctcagaaag gtgttcactc gactgagcac cgagttgaac aacaactcgt cgaagacgac
     1321 ccagcgatcg accggtcgat cgcgggtcga accctcaggg gcgtactcca tcgacaggac
     1381 cgtgtccgtg gcgattcggc caagcttttc gagatagatc agatagtttt cgatggtatt
     1441 gaagaccaga ttgatacggg caaattcgtt ggctgtggcg cgttggtccg ctccgctggc
     1501 tctgactcga gcctgaacga ccatcttcag ccgggagccg tcgaggttaa tggtgttgtc
     1561 gtcggcactc aatagagagg cgaacagggt tgcgaacatg aggatcacga aacccggacg
     1621 gggaaggggg aaagagaatc gcggaaccat gaggttactc cttcgcgtca gaactgcacc
     1681 gcggcttggc tggaggaatg ggaatgccga gagacgcaga gatttcctga accagtcctt
     1741 cgaccagttg atcggagggc aggtgtcgta gaatctgccc gtgtttgaag atgaggccgc
     1801 cgtctttggc gccggcgatc ccgaagtctg cctgacgggc ttctccgggg ccgttgacgg
     1861 cgcaacccat gacggcgatg cgtaccggtt gggtgagatg ggaaagtcgg tcacgaacgg
     1921 cttcagccat gcgcatgagt ggcacctggc aacgaccgca ggtcgggcag gagacgatga
     1981 ccggccccac ctgacggtga cccgtcacgc ggagaatccg gatgccggca gcgatttcgt
     2041 ccgcgctgtc gccggtcaga gagacgcgaa tcgtatcgcc gatcccttcc agcaggaggg
     2101 tgccgatgcc gatgctggaa cggatgaccc cctcatcggg aagaccgctt tcggtgacgc
     2161 cgaggtgcag cgggtaatcg caggcacgcg cgacctggcg cacggcctcg accatcagcg
     2221 aaacctcgaa tgccttgatg gcaatcttga tgttcgagaa cccctcggct tcgagcaagt
     2281 gcacttcctg caaagcggat tcggcgagcg cctgggctgt cactccacca tatcgctgag
     2341 ccatggcggg atcaagcgat ccggcgttga ccccgatgcg aatcggaatc ccgcgatcgc
     2401 gcgaagcggc aaccacctcg cgaactttct ggcgatcgcg caggttgccg gggttgatgc
     2461 gtagcccgtg aactccggct ttgaccgcaa gaatggcgag gtgatggtca aaatgaatgt
     2521 cggcgatgac gggaatcgga gcggcaggga cgatggttcg cagactctcg gccgcggcct
     2581 gatccggaac ggccaggcga atgatctcgc aaccggcgag cgagagacgt cgaatctcgg
     2641 tgatggtcgc ttcggcttga cgcgtgtccg ttttggtcat ggtctgaacc gacaccgggg
     2701 cgtctccgcc gaccgtgacc gtaccgacgc gaatggcgcg actgtgccgg cgtggtgcat
     2761 gagtcatggg atggagttct cctgaatcag gacgaagctg atgtcgtcct gtattgtagc
     2821 ggaagtttgg gtgattgtca gatgctggga caaacgttga tacaggtcgt gggccgaaca
     2881 acctgattga gccgcgagaa acgcgcgcag cccgtctccg gaatcatcac cgaacgcttt
     2941 ccagagacca ttggttgcca gacagacaat cgcaccggtc ccgggcgcga ccagggtggt
     3001 ggtcagccgg ggagcttcgt ccagcccaag cggagagccg ggatcgacca gggctcgtgg
     3061 aggctgtccc ggagccagca gaagtggggg tgggttcccg gcgcaactga aatgcagtcc
     3121 gtcagcggca aagacggcaa aggtcagcgc tgcccgtagt gggggttcgg aagaggcgcg
     3181 cagcaatcga tgcagaatca aaaaccgttg ctcggcggga gccgaacgca gggcggtgcg
     3241 aatgatgtac aaggtaagtg ctgcgggaac gccctgatga aaggcgtcac ataggcagag
     3301 aaatggaggc gagctgtcgg ggtcaagatc aatgagatca cctttgatcg tgtcggccag
     3361 agacgaaaaa agaaacagtc gccagtttcc cacgctatgg cagccattcg tggcttgaat
     3421 ctgccgatac atgcgcaagg tgagttccac ctcgcgtgcg gcccggtctc gctcgagcag
     3481 cgaggcaacc aggtgcagat tgtcgagaac cggtccgatt tcattcacga tactgccgat
     3541 caaagagcgt tcctgctcgg aatagggaag acgcgacgtc ttggcgctga gcaacagcag
     3601 accgacgcag gactgctcgc gaacgatcgg atacaacaat gtgaacccag cgctttgcca
     3661 ggcctgaaga accggtctgc agggcgccgc cgattcgaga gcgatttcca gcagggaatc
     3721 gatctcttcc gccttttccg tcagatcagt cggaatcgaa ctgaaagaaa cgctgactga
     3781 aggcgtgtcg aaaggccagg agactcggac accgtcagcc aggagcacga ccgcctgacc
     3841 gaaacccaga accggcgcac tttctcgcag caggttggcg cacacggttt cagcggtttc
     3901 cgctcggcgc atctcgcgca acagatgcct gatgaccgct gccgaatcga agtttcgccg
     3961 gccgaagagg tagtcgacga gcatgccgac cagcgatttc agtggattga aggccgccgc
     4021 g
//
LOCUS       DAORHD010000305         4000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24495_length_4000_cov_3.822307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000305 DAORHD010000000
VERSION     DAORHD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24495_length_4000_cov_3.822307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..635
                     /locus_tag="PKO06_07715"
     CDS             105..635
                     /locus_tag="PKO06_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNV69565.1"
                     /translation="MTDAELTRAILPIKLLLFDCDGVLADGAILHGSDGFEMKRFSAR
                     DGMGIDLWHRAGYLSGCISGRSAEALTKRGAELKFAELHQQVANKRAVLADILARRGL
                     DKSEVAYIGDDVNDLTMVGHVGLFMTPSDAHEEARKRADLVLSLPGGYGAIREAIDLL
                     LKTRRELEPLVQAYLG"
     gene            628..1521
                     /locus_tag="PKO06_07720"
     CDS             628..1521
                     /locus_tag="PKO06_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV69566.1"
                     /translation="MADLSWRDRFKQARRAARYWLVLSLVRLIENGPASRLPKLRRLL
                     LKIMPLVFARELQRARELLPAEFAAQTDAILEGMTFNQVTSILEVFLYEKLLAADAQF
                     ITVEGREHLDAARQAGRGIIILSGHYGSWELVAYTLIKLGYELHAIVRPQALNAMTEL
                     INGFRERRGVKVLMDNNLPSALKRLKKGGVVGIVSDLNARERGYRVSFFGREASFYHT
                     PIILAERTGAGLFPVSIERQTGGRHVIRIDPALSWDNSLPMPAKVQVYVNAFERIIRR
                     RPDHWVWFHERYRHAELGRTE"
     gene            1518..2285
                     /gene="lptC"
                     /locus_tag="PKO06_07725"
     CDS             1518..2285
                     /gene="lptC"
                     /locus_tag="PKO06_07725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNV69567.1"
                     /translation="MKGIFSSVGFWMLMLIIFLVVLMWRDDLEEARTKYVKQKMRLTD
                     VHFSEMDKGFEAVRFFADVVDMDDSQNNMIASRVRALFFDKQVATRSGELLASYAQRT
                     PYEIRFWGDVRLHTTDRERFRAEEARYFQNRRELFTTCPVTIWKDDMIITGKELRYNT
                     STREGELRRDVLIRIWPAASGSTPASGTVATAADEAPFWNLPPMRLATGAAVMPASMP
                     LASLPVTVPALSPATETTVASSVSTDTASHSSSRINR"
     gene            2301..3413
                     /locus_tag="PKO06_07730"
     CDS             2301..3413
                     /locus_tag="PKO06_07730"
                     /inference="COORDINATES: protein motif:HMM:NF015901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNV69568.1"
                     /translation="MKRNCSLYSCRWSPAIVWRFLGGLMLLAILLPAALFAMSSITAG
                     DMVITSRLMTMQGNVYVVRGGMKAEQKDSVLEADRGIYDRTIEVVKAFDNVRITQPGS
                     VLTADYLEAFVKEDRIVAQGNPKLVRIVERESKGEDGSVSKRKSRVILTCREVEGFNK
                     ENRFLAKGDVHVVEVAYREGETPQEAAENEKSPVSDLKCDSLEMFSREDKAIARDNVL
                     IVTKTLRATGDKAIYLDRENRLIIVGHAHGYQTGREAPGAQEQVSEIYANKIIYYPNE
                     DRTIAVGDVHATVYPKGQQSPFDDDAKKKKKKKKSEGETTATGTASTDSAGEDAEGGD
                     DDRRALEDAASRAGMPTPGPGDLTPVGDAVIEAQSE"
     gene            3410..>4000
                     /locus_tag="PKO06_07735"
     CDS             3410..>4000
                     /locus_tag="PKO06_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV69569.1"
                     /translation="MSTDSHNQLPASEAPQNAAVMSSSVTPVGAGQQVDEIQVAGVSK
                     TYSSRKVVDGASLHIRKGEIVGLLGPNGAGKTTTFYMVVGLIKPDSGTIHFNHREITG
                     LAMYQRARLGIGYLPQEASVFRKLTVKDNIRLILENVDIPEAERKSRMDRLIDELNIG
                     RIADSMGYALSGGERRRVEIARALAVEPSFILLDEPF"
BASE COUNT          845 a         1148 c         1212 g          795 t
ORIGIN      
        1 gaaaagcccg tcccacgcgg aaaatgtatg aatttggtct gtattgacta cttgcggaga
       61 aacggccgga atcgtagtat agaggccatt cgcaggagga acgcatgacc gacgccgaac
      121 tgacccgagc cattcttccc atcaagctgt tgctgtttga ctgtgacggg gttctcgccg
      181 acggagccat tctgcacggt tcagatggct ttgaaatgaa gcggttttcc gcgcgcgacg
      241 gcatgggtat cgatctgtgg cacagggcag ggtatctcag cggctgcatc tcgggccgtt
      301 ccgccgaagc cctcaccaaa cgcggcgctg aactcaagtt cgccgaactg catcagcagg
      361 tcgccaacaa acgcgccgtg ctggccgaca ttctggcgcg gcgagggctc gataaatccg
      421 aggtggccta tatcggcgat gacgtcaacg atctgacgat ggtcggccat gtcggcctgt
      481 tcatgactcc gagtgacgct cacgaagagg ctcgcaaacg ggctgatctg gtactttcgt
      541 tgccgggtgg ttacggagcc attcgtgaag ccatcgacct gctgttgaag acgcgtcgcg
      601 agcttgaacc cctggtgcag gcctatcttg gctgatcttt cctggcgcga tcgattcaag
      661 caggcgcggc gtgcggcccg ctactggctg gtgctgtcgc ttgtccggct catcgaaaac
      721 ggtccggcgt cgcgactgcc gaaactgcgt cgcctgctgc tcaagatcat gcctctggtg
      781 ttcgcccgtg aactgcagcg tgcccgtgaa ctgctgccgg ccgagtttgc cgctcagacc
      841 gacgcgatcc tcgaaggcat gaccttcaac caggtcacct ccattctcga agtctttctg
      901 tatgaaaaac tgctggccgc cgatgcgcaa ttcatcacgg tcgaaggcag agaacacctg
      961 gacgcagccc gccaggcggg gcggggcatc atcatccttt ccggtcatta cggcagctgg
     1021 gaactggttg cctacacgct gatcaaactt ggttacgagc tgcatgcgat cgtgcgtcct
     1081 caagcgctca atgccatgac cgaactgatc aacgggttcc gcgagcgccg gggcgtgaaa
     1141 gtgctcatgg acaacaatct gccgtcggcc ctcaaacggc tcaagaaggg tggggtcgtg
     1201 ggcatcgtca gcgatctcaa cgcccgcgaa cgtgggtatc gcgtgtcgtt cttcggtcgt
     1261 gaagcctcgt tctatcacac tccgatcatt ctggccgagc gaaccggcgc cggactgttt
     1321 ccggtttcca tcgaacgtca gaccggtggt cggcatgtga tacgtattga tcctgccctg
     1381 agctgggaca attctctgcc gatgcccgcg aaagtgcagg tctacgtaaa cgcgttcgaa
     1441 cgcatcatca gacgccggcc cgaccactgg gtctggtttc acgagcgcta ccgccatgcc
     1501 gaactgggga ggacggaatg aagggaatct tctctagcgt cgggttctgg atgctcatgc
     1561 tgatcatctt cctggtggtc ctgatgtggc gcgacgatct cgaagaggcc cgcaccaaat
     1621 atgtgaaaca gaaaatgcgc ctgaccgacg tgcatttcag cgaaatggac aaagggttcg
     1681 aggcggtgcg gtttttcgcc gacgtggtcg acatggatga ctcgcagaac aacatgatcg
     1741 cctcacgggt gcgcgccctg ttcttcgaca aacaggtggc aacccggtcc ggagagctgc
     1801 tggcttcgta tgcccagcgc accccgtatg agattcgatt ctggggtgat gtgcgactgc
     1861 acaccaccga ccgcgaacgc ttccgtgccg aagaggcgcg ctacttccag aatcgcaggg
     1921 agctgtttac cacctgtccc gtgacgatct ggaaagatga tatgatcatc accgggaagg
     1981 aattgcggta taataccagc acccgggaag gggaactgcg gcgcgatgta ctgattcgca
     2041 tctggcccgc ggccagtggt tcgacgcccg ccagcggaac ggtggccacg gctgccgatg
     2101 aagcaccgtt ctggaatctg ccgcccatgc gactggcaac cggcgcagcg gtaatgccgg
     2161 cttcgatgcc tctggcttcg ctgcctgtca cggttccagc actgtcgccg gcgacggaga
     2221 cgacggtcgc gtcgagtgtc agtactgata ccgcgtcaca ttcttcgagt cgaatcaatc
     2281 gataacagga tctgaacgat atgaaacgaa actgcagctt gtactcctgc cgctggtcgc
     2341 ccgcgatcgt ctggcgattc ctcggggggc tgatgttgct ggcgattctg ctgcccgcag
     2401 cgctgttcgc catgagttcg atcacagcgg gtgacatggt catcaccagt cgtctgatga
     2461 ccatgcaggg gaacgtctac gtcgtgcgcg gcggcatgaa agccgagcag aaagattcgg
     2521 tgctcgaagc ggaccgtggc atctacgacc gcacgatcga ggtcgtcaag gcgttcgata
     2581 atgtgcgcat cacgcagccc ggatccgtgc tgacggccga ctatctcgaa gccttcgtca
     2641 aggaagatcg catcgtcgcc cagggcaacc ccaagctggt acgtatcgtc gagcgtgaat
     2701 cgaagggcga agacggctcc gtttccaagc gcaagtcgcg ggtgatcctc acctgtcgcg
     2761 aagttgaagg ctttaacaag gaaaaccggt tcctggccaa aggcgatgtt catgtggtcg
     2821 aagtcgccta ccgcgaaggc gaaaccccgc aggaagcggc ggagaatgag aaatctccgg
     2881 tttctgatct caaatgcgat tcactcgaaa tgttctcgcg cgaagacaag gcgattgccc
     2941 gcgacaacgt gctgatcgtg actaaaaccc tgcgcgccac gggcgacaag gcgatctatc
     3001 tcgaccgcga aaaccgtttg atcatcgtcg gccacgcaca tggctaccag accggtcgcg
     3061 aggctcccgg ggcgcaggaa caggtgtctg agatctacgc gaacaagatc atctactacc
     3121 ccaacgaaga tcgtacgatc gcggtgggtg atgtgcatgc gaccgtgtat cccaaggggc
     3181 agcagagccc attcgatgac gatgccaaga aaaagaagaa gaagaaaaag tccgagggcg
     3241 aaacgactgc aaccggcacg gcaagcactg attcggcagg cgaagatgca gagggtgggg
     3301 acgacgatcg tcgcgcgctc gaggatgccg cttcccgcgc gggtatgccg actcctggtc
     3361 cgggagatct gactcccgtc ggtgatgccg tcatcgaggc gcagtcggaa tgagtaccga
     3421 ttcgcacaac cagttgcccg catctgaagc tcctcagaat gctgcagtca tgtcgtcctc
     3481 ggtgaccccc gtgggcgcgg gacagcaggt cgatgagatt caggtcgccg gggtcagcaa
     3541 gacctactcc tcgcgcaaag tcgtcgatgg ggcctcgttg cacattcgca aaggcgagat
     3601 cgtcggtctg ctggggccga acggagccgg aaaaaccacg acgttttata tggtggtcgg
     3661 cctgatcaaa cccgattccg gaacgattca tttcaatcat cgcgagatca ccgggctggc
     3721 catgtaccag cgggctcgac tcggaatcgg ctatttgccg caggaagcgt cggtgtttcg
     3781 caagttgacg gtgaaagaca atattcgtct gattcttgaa aatgtcgaca ttcccgaagc
     3841 ggaacgcaaa agccgcatgg atcgtctcat cgacgagctg aacatcggtc gtatcgccga
     3901 tagcatgggc tatgcgctct cgggcggcga acggcgacgc gtcgagattg cccgcgcgct
     3961 ggcggtcgaa cccagcttca ttctgcttga cgaaccgttt
//
LOCUS       DAORHD010000306         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24592_length_3989_cov_3.901627, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000306 DAORHD010000000
VERSION     DAORHD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24592_length_3989_cov_3.901627"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKO06_07740"
     CDS             complement(<1..778)
                     /locus_tag="PKO06_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69570.1"
                     /translation="MSDTVIPSLSQENPAETVNCCWVAPSASLGKRLLLSLLALTIAS
                     LIWIPQAHRLYRVRLDDYRQPSGVPPKVRAIAARHLRLWTDPTLRKQEIGRMRGSNAE
                     WDFMGRTFFVMALANLCYRDHASQTVYLEIIDRIIEETVRIEREKGMFFFLMDYGRSQ
                     PFLASPARSIFVDGEIALMMAARQAVAPRDDFRPMLTERVEILVGQMGSGTAMCGESY
                     PNECWMFCNTVAAAAIRMHDIIEKSDHSAFLQRWLTTVREK"
     gene            complement(783..1325)
                     /locus_tag="PKO06_07745"
     CDS             complement(783..1325)
                     /locus_tag="PKO06_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNV69571.1"
                     /translation="MQIPYRSLLPQVHSSVFLAEHTIVAGDVHLEENVNIWYGVTIRA
                     DLAAIVIGRDTNVQEQTVIHVDHPTAEWDGRTVIGNEVTIGHRALLHSCRIGDRCLIG
                     MGAIILSGAEIGEGSIVGAGAVVREGTKIPPRSLVVGLPAIIKGTVSPDRYEYIRASA
                     RRYVELAQEYKLNQKHTSAG"
     gene            1443..3911
                     /locus_tag="PKO06_07750"
     CDS             1443..3911
                     /locus_tag="PKO06_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV69572.1"
                     /translation="MRLICRRLSQFPAAGLAGGKAQGLKLAMDAGLRIPLTWVISVEH
                     SSEVSSSVPDASSLTPLLDEVLHDLQRAGTVSLAVRSSALAEDGRYVSFAGQWRSYAR
                     VPLRIEAVVDALRGVFAAGGSASLREYADQFGISLAASGVAALIQPWVDARVSGAAFS
                     VNPLTGERQIIAEAVAGTAEALMGGCANPQRYLLTEGTHCTGEGEASVLLAPSHQTIL
                     KSAVEKLERAAGWPVDIEWAMDAEGLVILQVRPVTAFAVPPSPRIAWSRELTAERYPQ
                     PLSPLGWTNIKAVFEEGVRSFADFMGMPLEVQTELAAIVDGWVVANPEAFDFKQRFRL
                     QLKADESVQVLGVMIRYGLSRWRTAGLRALMADIKELRRCLVQQERVRTRLGQRPGRL
                     AHVVIGTALSLYLRRVADEVRTEWPAVLQQFRDEVAAVETALTNTDDPRRLIDLGDRL
                     RRAMIAYVRPDLVIFAVKEIASLILREAAVLNGMADAPRIPALLGRGLSDNCTLRFHQ
                     DLARLRAQLVTSGIVFNMTPMLDDSRIRDFLRRYGHQTTSWDIMIPTLGEQPERLLDL
                     LRLSGSERPDPHEQSWPDIAQEQGRILAQLGQTAWQRTLADEACEIMRSFMSIDEEHH
                     FHTGLIIPVTRRLVLKIGETLRQRGVIRDAAEVFWLTDGELRETFLSGDRRSKLPLIR
                     QRELAWRQACRRGPPKLARASDEAATHSDSDGLSGLGVSAGVARGRVRKIGGLADLVD
                     LQPEEILVVRAPDPTLACTFGKIAGLVAETGALLSHGAVAAREYHLPAVFGCRDAFST
                     FVDGELIEIDGTRGTILRREPPVT"
BASE COUNT          766 a         1104 c         1280 g          839 t
ORIGIN      
        1 gtttctcgcg cacggtcgtg agccagcgct gcagaaaggc ggaatggtca ctcttctcga
       61 tgatgtcatg catgcggatg gccgcggctg ccaccgtgtt gcagaacatc cagcactcgt
      121 tcgggtagct ttcgccgcac atggctgtcc cgctacccat ctgacccacg aggatctcga
      181 cccgttccgt cagcatcggg cggaaatcat cacgtggagc gactgcctgg cgcgcggcca
      241 tcatcagagc gatctccccg tcgacaaaga tgctgcgggc gggactggca agaaagggct
      301 ggcttctgcc gtaatccatg aggaagaaga acatgccctt ctcgcgttca atgcgcaccg
      361 tctcctcaat aatgcggtcg atgatctcga gatagacggt ctgcgacgcg tgatcgcgat
      421 aacacaggtt tgccagcgcc atgacgaaaa acgtgcgacc catgaagtcc cactcggcgt
      481 tgctgcctcg catgcgtcca atctcctgct tccgcagggt tggatcggtc cagagacgga
      541 gatgtcgggc ggcaatggct cgtactttcg gcggcacccc ggatggttgt cggtagtcgt
      601 ccagacgcac ccgatacagt cggtgtgcct gcggaatcca gatcagggac gcgatcgtca
      661 gagcgagaag tgacagcagt aaacgcttgc ccaacgaagc ggaaggggcg acccagcaac
      721 agttgacggt ctctgctgga ttttcctgag atagcgatgg aataacggtg tcgctcaaaa
      781 gatcatcctg cagatgtgtg cttctggtta agcttgtact cttgagccaa ctcgacgtag
      841 cgccgagctg aggcgcgaat gtattcgtac cggtcgggcg aaacggttcc tttgatgatt
      901 gccggcaaac cgaccacaag agaacgaggc ggaatcttcg tgccttcgcg tacgacagcg
      961 ccggcaccaa cgatcgaccc ctcaccgatt tcggctccgc tgagaatgat cgcgcccatg
     1021 ccgatcaggc aacgatcgcc gattctgcag gaatgcagca gcgcgcgatg gccgatggtg
     1081 acctcattgc cgatgaccgt gcgtccatcc cactcggcag tggggtgatc gacgtgaatg
     1141 acggtttgtt cctgaacgtt cgtgtcacgc ccgatgacga tcgcggccag atcggctcga
     1201 atcgtgacgc cgtaccagat gttgacgttt tcctcgaggt ggacgtcacc ggcaacaatc
     1261 gtgtgttcgg ccagaaagac agaggaatga acctggggga gcagagatcg atacggaatc
     1321 tgcatgaaac actccgacga aaaggcaggt gggcgcgaga cgaacctggt tcgcgcaggg
     1381 catcggtatg ataccattgt ttggcacgaa tgccgaatca atccggcgag agtggagtgg
     1441 tcatgcgact gatctgtcga cggctgtctc agttccccgc cgcagggctg gcgggtggaa
     1501 aggcacaggg cctgaagttg gccatggatg ccggattgcg cattccgctc acctgggtga
     1561 tctccgttga gcattcgagt gaggtgagca gttccgtacc ggatgcttcg agtctgacgc
     1621 cgctgctcga cgaggtattg cacgacctgc agcgggcagg aaccgtcagt ctggcggttc
     1681 gttcgtcggc tttggcggaa gatggccgct acgtcagttt tgctggtcag tggcggagtt
     1741 atgcgcgagt gcccctgcgc atcgaagcgg tggtcgatgc tctgcgcggc gtctttgcgg
     1801 ctggtggcag tgcgagtctg cgagaatacg ccgaccagtt tgggatctcg ctggccgcat
     1861 ccggagtggc cgcattgatt caaccatggg ttgatgcccg cgtttcaggc gccgcgtttt
     1921 ccgtcaatcc gctgaccggc gaacgacaga tcatcgccga agccgtcgcc ggaaccgcgg
     1981 aagccctcat ggggggctgc gcgaaccctc agcgatacct gctgaccgag ggaacgcatt
     2041 gtacgggcga gggcgaagcg tccgttctgc ttgcgccgtc acatcaaacg atcttgaagt
     2101 ctgccgtcga aaagctggag cgcgcggcag ggtggccggt cgatatcgag tgggcaatgg
     2161 acgcggaggg attggtgatc ctgcaggttc ggccggtgac ggcctttgcg gtgccgccgt
     2221 ctccccgaat cgcctggagt cgcgagctga ccgcggaacg gtatccgcag ccgctcagcc
     2281 cattgggctg gaccaacatc aaagcggtgt ttgaggaagg ggttcgcagt ttcgctgact
     2341 tcatgggcat gccgctcgag gttcagacgg aattggccgc gattgtcgat ggctgggtgg
     2401 tggcaaatcc tgaagcgttc gatttcaagc aacggtttcg tctgcaattg aaagcggacg
     2461 aatctgtaca ggtgcttggc gtgatgatcc ggtatggcct ctctcgctgg cgaactgctg
     2521 gtcttcgtgc tctcatggca gacatcaagg aactgcgtcg ctgcctggtg cagcaggaga
     2581 gggttcgcac ccgtcttggc cagcggcccg gtcgcctggc tcacgtggtc atcggaaccg
     2641 cattatcgtt gtatctgcgg cgcgttgctg acgaggtgcg aacagagtgg cccgcggtgt
     2701 tgcagcaatt ccgcgatgag gtggccgcgg tcgagactgc tctgacgaac acggacgatc
     2761 cccggcgatt gattgacctt ggcgatcgtc ttcgccgggc gatgatcgct tatgtccgac
     2821 cagatctggt gatcttcgcg gtcaaggaaa tcgcttccct gattctgcga gaagccgcgg
     2881 ttctgaacgg catggccgat gccccgcgaa tccccgctct tctgggccgc ggcttgtcgg
     2941 ataattgtac cctgcgattt catcaggatc tggcacgact tcgggcgcag ctggtcacct
     3001 caggtatcgt tttcaatatg accccgatgc ttgacgattc gcggatcaga gactttttgc
     3061 gccggtacgg tcatcagacc accagttggg acatcatgat tccgacgtta ggggaacaac
     3121 cggagcgcct gcttgatctt ctgaggctca gtggaagtga gcgtcctgac cctcatgaac
     3181 aatcatggcc agatattgct caagaacagg gaagaatact tgctcagctg ggacaaacgg
     3241 cgtggcagcg tacgctggct gacgaggcgt gtgagatcat gcgctctttc atgagtatcg
     3301 atgaggagca tcattttcac accggattga tcattccggt taccagacgg ctggtcttga
     3361 agatcggtga aacgctgcgg cagcgtggtg tgatcagaga cgccgccgag gtcttctggc
     3421 tgaccgatgg cgaactgcgc gagacatttc tgtccgggga ccggcgttcg aaactgccgc
     3481 tcattcgaca gcgtgagctg gcatggcgtc aggcctgtcg ccgcggaccg ccgaagctcg
     3541 cgcgagcgtc agacgaggcg gcaacccact cagattcaga cgggctctcg ggacttggag
     3601 tgagcgcagg ggttgctcgc gggcgagtgc ggaagatcgg cggtctggcg gatcttgtcg
     3661 atctgcaacc cgaagagatt cttgtggtga gggctcctga cccaactctc gcctgtacct
     3721 tcggcaagat tgcaggcctt gtcgccgaga cgggggcgtt gttatctcac ggggccgtgg
     3781 ctgcgcgcga gtatcatctg ccggcggtgt tcgggtgccg tgacgcgttc tcgaccttcg
     3841 tggacggtga gttgatcgaa atcgacggca cacgcggaac gattttgcga cgggagcccc
     3901 cggtgacatg agttttcagc cccgtatcat tctctgggat ctcgatggaa cgctggcctt
     3961 ctggcagagt ccacgcatca tgtgtgaca
//
LOCUS       DAORHD010000307         3987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24606_length_3987_cov_4.064852, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000307 DAORHD010000000
VERSION     DAORHD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24606_length_3987_cov_4.064852"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1968
                     /locus_tag="PKO06_07755"
     CDS             <1..1968
                     /locus_tag="PKO06_07755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV69573.1"
                     /translation="ADHGSIMVFDPMSKKLRLVRSADHPGTTAPKLVNAGAGIAGTVF
                     ETGKAILVSDLQAAPPGVPLWRPDRGGSFMSLPLKIAGRMIGVLNLERDHGRDPFGHR
                     ELREVEAVAAQIASIIEKGRNLEEMRRGRNEFEALYVMIGKLFSGSPFRNALQSCLVE
                     LSSRLNLERSAVVQFESQPDSDDETFILLAAHQLSERGLKKIVTEIRERFERQLSERG
                     PEAEDSAIPSTLAFTERGRLMELFCLPLIVKDAARHFLLVSRQADLGDETATAHHYRF
                     LWMLSKQLAAAIEREEMIQELKENERLLAQEADADKLSLMISTGLASSLDPHLILSKA
                     FQGFHRLIPYVTALVLIYDDLERQYRIMLFVAYRMSASYRRSLLEQLQRIITEEYVLD
                     PPLNELSGVPIEMISTPATGDGVASKYQYSMYQPLILDDKIKGILLLTRKNGPEFSPY
                     DQARMSQFNNMLMASVKNALIHRRTEKLAYTDPLTGQFNHRYFQETLTQEFARARRYT
                     NPLSLMILDIDHFKKFNDTYGHLIGDRVLIHAAQVFKRSIREKIDTVARYGGEEFAVL
                     LPETGIDGARRFAERIRQALESEGLTTEFGDLRVTISIGVACTIVTQCNKPSDLIDAA
                     DQALYFAKDSGRNQVQVYQKETIDHDNTKKR"
     gene            1946..>3987
                     /locus_tag="PKO06_07760"
     CDS             1946..>3987
                     /locus_tag="PKO06_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV69574.1"
                     /translation="MTTRKNAETRLTTRKNGDALNRLTRLSDLSMALSTGTDLETLLR
                     KLAASVRESFHADEAFFLLLDVDSFELVKSVYSGKARKRPTSIPCPERRLGLTSPGEY
                     GFAPCVHQSENAGESDLSSEARLYRSVFVSRRRLLFQRRDLDAAYGDVPFQSALVMPL
                     LTASQALGLLVVSHWEQVDGFSNTDLEEATVYANLAALAVEHMKLIREREARIREMEK
                     LNTLAKRFSTVQTLEGLIGLSFEYLSQIISHELGVVVLFSGVEETRYFVANRVLTPTN
                     LENLTAHIKEIADNLRGRSAKLVRSQQIFLPTKEGSPMGRMFRGRLQSFLTVPLTVQG
                     KNIGLVNLSSILHNSFNREHLRAFTTLSNLLATAIENVKIRLYLERRVDEMSVLFEVS
                     QSLTSTLRQEEVLDLIVNFSMEMTNALRCELRLLDTAGQLLEIKAARGLSKSFLSRTP
                     IRVGEGILGQVISERKPISVVDVRKDPRTKHLQVVKREKLAGLLAVPIMQRQKPVGVI
                     SIYTSKPRDFTQAEIDLLTTFASQASIAIENARLYEKMKDQYLSMVMALAAAIETKDA
                     YTHGHSKQVMEYAIKIARELELSDDEIETIKYAGLLHDIGKIGVRDVILSKEGPITAE
                     ERAELKNHPQYGAFIMEQVEFLKDIAPLTLYHHEKWDGIGYPLGLKGDKIPLGARIL"
BASE COUNT          867 a         1102 c         1158 g          860 t
ORIGIN      
        1 gcggaccatg gctcgatcat ggtgtttgat ccgatgagca agaagctgcg cctggtacgt
       61 tccgcagatc atccgggaac gaccgcgccg aagctggtga acgcgggggc cggaatcgcc
      121 ggcaccgttt tcgagaccgg caaggcgatc cttgtcagcg acctgcaggc ggctcccccg
      181 ggagttccac tgtggcgccc tgatcgcggt ggttcattca tgtcgctgcc gctgaagatc
      241 gccggtcgca tgatcggcgt gctgaatctc gaacgtgacc acggtcgaga tccgttcgga
      301 catcgggaac ttcgcgaggt cgaagccgtg gcggcgcaga tcgcctcgat catcgagaag
      361 ggtcggaacc tcgaagagat gcggcgtggg cgaaacgagt ttgaagcgct ctacgtcatg
      421 atcggaaaac tgttttccgg aagtcctttc cgcaacgccc tgcagagctg tctcgtcgaa
      481 ctttcttcac gacttaatct tgagcgttcg gcggtagtac aatttgaatc tcaacccgac
      541 tcggacgacg agacttttat cctgctggcg gcgcaccagc tttctgaacg cggcctgaag
      601 aaaatcgtga ccgagattcg cgaacgcttt gaacgacaac tgagcgagcg aggtcccgag
      661 gcggaagaca gcgctattcc ctcgaccctg gccttcaccg agcgcggtcg tctgatggaa
      721 ttgttctgcc tgccgctgat cgtgaaagat gctgctcgcc attttctgct ggtcagccgt
      781 caggccgatc tgggcgatga gaccgccaca gctcatcatt accgcttcct ctggatgctc
      841 tcgaagcaat tggcggcggc cattgagcgc gaagagatga ttcaggaact gaaagagaac
      901 gaacgtctgc tggcgcagga agccgatgcg gacaagctgt cgttgatgat cagtaccggg
      961 ttggcctcat cgctcgatcc gcatctgatt ctgtcgaagg cgtttcaggg ctttcaccgg
     1021 ctgattccgt atgtgacggc gttggttctg atctatgacg atcttgagcg gcagtaccgg
     1081 atcatgctgt tcgtggcgta tcgcatgagt gcctcctacc ggcgctcact gctggaacag
     1141 ttgcagcgca tcattaccga agagtatgtg ctcgatccgc cgctgaacga gttgagcggg
     1201 gttccgatcg agatgatatc gacccctgcc accggagatg gggtggcgtc gaagtatcag
     1261 tattccatgt accagccgct gattcttgac gacaagatca aaggcatctt gttgctcact
     1321 cgaaagaacg ggcctgagtt cagcccgtac gatcaggccc gcatgtctca gttcaacaat
     1381 atgctgatgg ccagcgtcaa gaatgcgctc attcatcgtc gcacggaaaa actggcctac
     1441 acggatccac tgacggggca gttcaaccat cggtattttc aggaaaccct gacgcaggaa
     1501 tttgcccgtg cccgacgcta taccaatcca ctgtcgctga tgattcttga tatcgatcat
     1561 ttcaagaagt tcaatgatac atacggtcat ctgatcggcg atcgggtgct gattcacgcg
     1621 gcacaggtgt tcaaacgcag tattcgcgag aagatcgata cggtggcccg gtacggcgga
     1681 gaagagtttg ccgtgctgct gcccgagacc ggaatcgacg gggcgcgacg gttcgcggaa
     1741 cgcattcggc aggctctcga gtccgagggg ttgacgaccg agttcggtga tctgcgtgtc
     1801 acgatttcga ttggggtggc gtgcacgatc gtgacgcaat gcaacaaacc cagcgatctg
     1861 atcgacgcgg cggaccaggc gttgtatttc gccaaagaca gcggccgcaa ccaggtgcag
     1921 gtgtatcaga aggaaaccat cgaccatgac aacacgaaaa aacgctgaaa ctcgcttgac
     1981 gacccggaag aacggcgatg ccctgaatcg cctgacccgg ctttccgacc tgtcgatggc
     2041 tctttccacc ggcactgatc tcgagacctt gctgcgcaaa ctggcggcct cggtgcgcga
     2101 gagctttcat gccgacgaag cgttttttct gctgctggat gtcgactcgt tcgagttggt
     2161 gaagagtgtc tattcgggca aggcccgcaa acgtccgact tcgattccgt gccctgaacg
     2221 tcgactcggg ttgacttcgc ccggggaata cggatttgcg ccctgcgttc atcagtcgga
     2281 aaacgctggc gagagcgatc tttcgagcga ggctcgcttg taccgaagcg ttttcgtttc
     2341 tcggcgtcgt ctgctgtttc agcgtcgcga tctggatgcc gcgtatgggg atgttccgtt
     2401 ccagtcggca ctggtcatgc cgttgctgac cgcgtctcag gctctcggtc tgctggtcgt
     2461 cagtcactgg gagcaggtgg acggcttttc gaacaccgat ctcgaagagg cgaccgtcta
     2521 cgcgaatctg gcggcgctgg ccgtcgagca catgaaactg attcgcgagc gcgaggcccg
     2581 catacgcgaa atggaaaaac tcaacacgct ggccaagcgc ttttccaccg tccagaccct
     2641 cgaaggtctg atcggcctgt cgttcgagta tctctcacag attatctcgc acgagctggg
     2701 cgtcgtcgtg ctgttttccg gtgtcgaaga aacgcgctat ttcgtggcga accgcgttct
     2761 gacgccaacg aacctcgaaa acctgacagc acacatcaag gaaatagccg acaatcttcg
     2821 tggtcgttcc gcaaaactgg tacgatcgca acagatcttt ctccccacga aggaaggctc
     2881 gccgatgggg cgcatgttcc gtgggcgact ccagtccttc ctgaccgtgc cgttgacggt
     2941 gcagggaaag aacatcggcc tggtcaacct gtcgtcgatt ttgcacaatt cgttcaatcg
     3001 ggaacacctg cgggcgttta ccaccttgtc gaatctgctg gcgacggcca ttgagaacgt
     3061 caagatcaga ctgtatcttg aacgccgggt tgacgaaatg tccgtgctgt ttgaagtgtc
     3121 ccagtcgctg acctcgactc tgcgccagga agaagtgctt gatctgatcg tgaacttctc
     3181 catggagatg accaacgcgt tgcgctgcga actgcgactg ctcgacactg ccggacaact
     3241 gctcgagatc aaggcggcac ggggactgtc caagtcgttt ctgagtcgaa ccccgatcag
     3301 agttggagaa ggcattcttg gacaggtcat ttcggaacgc aagccgatca gcgtggtcga
     3361 tgttcgcaaa gacccgcgaa ccaaacattt gcaggtggtc aaacgcgaaa aactggccgg
     3421 actgctggct gtaccaatca tgcaacgcca gaaaccggtc ggagtcattt cgatctatac
     3481 cagtaaaccg cgcgatttta cgcaggctga gatcgatctg ctgacgactt ttgcgtcgca
     3541 ggcgagtatc gccatcgaga acgctcgact gtacgagaag atgaaagatc agtatctgtc
     3601 gatggtcatg gcgctggcgg cggcgatcga aaccaaagac gcgtataccc acggccacag
     3661 caaacaggtc atggaatacg ctatcaagat tgcacgcgag ctcgaactct ccgacgacga
     3721 gatcgaaacg atcaaatacg ccggtctgtt acacgacatc ggcaagatcg gtgtgcgcga
     3781 tgtcattctt tccaaggaag gtccgatcac ggccgaagaa cgtgccgagc tgaagaatca
     3841 tccgcaatac ggcgcgttca ttatggaaca ggtcgagttt ctgaaagata tcgccccgct
     3901 gaccctctac caccacgaaa agtgggatgg tatcgggtat ccgcttgggc tgaagggtga
     3961 caagatccct ctgggcgccc gtattct
//
LOCUS       DAORHD010000308         3980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24675_length_3980_cov_3.887643, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000308 DAORHD010000000
VERSION     DAORHD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24675_length_3980_cov_3.887643"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKO06_07765"
     CDS             <1..247
                     /locus_tag="PKO06_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69575.1"
                     /translation="AVTGEPIVRATTEGLDFAYELRNLPDLAPVPIIIVSGMMQSPEY
                     PRQFEKILGRDWPAARFLEKPVPPVQLLEEVKKVIRW"
     gene            286..708
                     /locus_tag="PKO06_07770"
     CDS             286..708
                     /locus_tag="PKO06_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69576.1"
                     /translation="MAHKILLVDDDPDFTAIHKEILEKDGFIVSVIHNSVEAAEALES
                     GKETPDLIMTDLMMEKDDSGFKFCHRVRRNAKTAKTPILMCTGVARARSLGFDIKSPE
                     AREWIKADDFIEKPIRAETLLAKVHHLLGEPATTTNHH"
     gene            730..2256
                     /locus_tag="PKO06_07775"
     CDS             730..2256
                     /locus_tag="PKO06_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69577.1"
                     /translation="MGPTPGKIRILVADDEMSIRLGCEKILQSEGFEVVTVDNGLTAL
                     ERFDEKPFDLVLLDLMMPGMNGLAVLAEIKARDSDVVVIMITGYASFETAVKAIQDGA
                     YDYVPKPFTPGELKIVVKRALERRHLLLRNRRLQEERERTLRDLALEQSRVRSILNAM
                     PEPLLVFNAERELVLFNPAGRVFLKNLTDLIGKNMSEAISLPNLITALDEVWPKLGRK
                     IEAVAVEVEDPDSKQTWLLNADALSEDKGGEFAEGGTLRGAVVVLSNITPMKDLERAK
                     SRFVSIVAHELKAPVAAIEGYLELIIGDLEEAMEKFRPKLERCRDRANLLQRLIRELL
                     DISRIEQGRIERNLERLDPMSIVTEVAEFHQLEATKRGISITHEPPKQPVSIRADRTE
                     VNRIFTNLVSNAIKYNKEKGSIVINCGMHGAFWRCSVTDTGIGIPRNHLINIGEEFFR
                     VKNSQTVQISGTGLGVSIVKRLLDLNHARLMIASEEGEGSTFTVYWPTDACATTQQKS
                     "
     gene            complement(2276..>3980)
                     /locus_tag="PKO06_07780"
     CDS             complement(2276..>3980)
                     /locus_tag="PKO06_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69578.1"
                     /translation="ITCGRGRDSGVEIDTIATLRGEADVTIRNSGEHFSVGEGQQLII
                     RGGQVTQQNIDTNQLGNQWNNQLQNMGGSVDFGDIPGILRNIRENEGTLIRNLRSSLE
                     GATRESAPALLKAVDRAIGVLDEDAMIMNNFMRRVRESSSRPDQGLVSAIADCLRSNA
                     SYRTDVGAMLRQLRQIESLDLGEIPGRLESLRSSLANGMTAIREANAAIPDGTAANAP
                     AMLELRQRVAEYQQTLSAAEAEIGRMQRQLADMINAGQGNLQQARALARQAASLSDAI
                     GGYRQELRRISQRGGSAGSVNIEAEQAAIQELGDRVIALGEEMAGLSDEVGRIREVFE
                     MSRAAVLTPGQAQSVYLDAREALNAIIQGDLADVAADVEELRTDLQSLTEQKAQLIDR
                     MAEKLASSLVLRNLVNDLNRRLSDLANQLNTIGTDLTKFKTEQASLLSSLNVPTIDES
                     VVTQLQDAEEAMSDNIILFENELRQYANSGVSARDILKSSVQVLRSFQRARRLYISAQ
                     RLYESVTRGAGRGTATQELEAVQAIWERISDEYQRLSNAAGTLEAELTSLENQLNTLT
                     N"
BASE COUNT          870 a         1225 c         1064 g          821 t
ORIGIN      
        1 cgccgttaca ggtgaaccta ttgtgcgtgc cactacggaa gggctcgact ttgcgtatga
       61 actgcgtaac ctgcccgatc tggcacccgt tccgatcatt attgtttccg gcatgatgca
      121 atcccccgag tatcctcgcc aattcgagaa gatcctcggg cgcgactggc ctgccgcacg
      181 ttttctcgag aagcctgttc cgccggttca gctgctcgaa gaagtgaaaa aagtcattcg
      241 gtggtagaat caccactcag ttagatcacg taaggagact ctgtcatggc acacaagatt
      301 ctgctcgtgg acgacgatcc cgactttacc gccatccaca aggaaatcct ggagaaagac
      361 ggattcattg tttcggtcat tcacaactcc gttgaagccg ccgaagcgct tgaatccggc
      421 aaggaaactc ccgatctcat catgaccgac ctgatgatgg aaaaggacga ttccgggttc
      481 aagttctgcc accgggttcg ccgcaacgcc aaaaccgcga aaacccccat tctgatgtgc
      541 accggtgtcg cccgcgcccg cagtctcggg ttcgacatca agtcacccga agcccgcgaa
      601 tggatcaagg cggatgactt catcgagaaa ccgattcggg ccgaaactct gctcgccaag
      661 gtccaccacc tgctcgggga accggcgacc acgacgaatc accactgatc agaaacgagg
      721 gagaagcaca tgggtccaac gccaggcaag attcgtatcc tggtcgccga tgacgaaatg
      781 tcgattcgcc tcggctgtga aaaaattctg cagtccgagg ggttcgaggt cgtcacggtc
      841 gataacggcc tgactgcgct cgaacgattc gacgaaaagc cgttcgacct tgtgcttctc
      901 gacctcatga tgcccggcat gaacgggctt gccgtcctcg ctgaaatcaa ggcacgcgac
      961 tccgatgtcg tcgttatcat gattaccggg tatgccagct ttgaaaccgc cgtgaaggcc
     1021 attcaagacg gggcctatga ttacgttccc aagccgttca cacccgggga actcaagatc
     1081 gtcgtcaagc gtgcgctcga gcgacgccat ctgctgctgc gtaaccgccg cctgcaggaa
     1141 gagcgcgagc gcacgctgcg cgacctggca ctggaacaga gccgcgttcg ttcgattctc
     1201 aacgccatgc ccgagcctct actggtgttt aacgccgagc gcgaactggt actcttcaac
     1261 cccgccgggc gcgtctttct gaagaatctg accgacctga tcggcaagaa catgtccgag
     1321 gccatttcgt tgccgaacct gatcacggcc ctcgatgaag tctggccaaa gctcggtcgc
     1381 aagatcgaag ccgtcgcggt tgaggtcgaa gaccctgaca gcaaacaaac ctggctgctg
     1441 aatgccgacg cgctgagcga agataagggc ggagaattcg ccgagggcgg cactcttcgt
     1501 ggtgccgtgg tggtactgtc aaacatcacc ccgatgaaag atctcgaacg ggccaagtcg
     1561 cggttcgtgt cgattgtcgc tcacgaactc aaagctccgg tcgccgccat cgaaggctat
     1621 ctcgagctca tcatcggcga tctcgaagaa gcgatggaga agttccggcc caagcttgaa
     1681 cgctgtcgtg atcgggccaa tctgttacag cgattgattc gcgaactgct cgatatctcg
     1741 cgcatcgagc agggtcgcat cgaacgcaac ctcgagaggc ttgacccgat gtcgatcgtg
     1801 accgaggtcg ccgagtttca tcaactcgaa gccacgaaac gcggcatcag catcacgcac
     1861 gaacccccga aacagccggt ctcgattcgc gccgaccgca ccgaggtcaa tcgcattttt
     1921 acgaacctcg tgtcgaacgc catcaagtac aataaagaga aggggtcgat cgtcatcaac
     1981 tgcggcatgc acggggcatt ctggcgttgt tcggtcaccg acaccggcat cggtattccc
     2041 cgcaatcatc tgatcaacat cggcgaggag ttctttcgcg tgaagaactc acaaaccgtt
     2101 cagatcagtg gcaccggtct cggcgtctcg atcgtcaaac gactgctcga cctgaatcac
     2161 gcccgcctga tgatcgcgtc agaagaaggc gaaggctcga cgttcaccgt ctactggccg
     2221 acggacgcgt gcgccaccac gcagcagaag agctgacccg ttttcgagtc gcgtctcagt
     2281 tcgtcagcgt gttgagctga ttctccaggc tcgtcagttc agcctcgaga gtgccggccg
     2341 cgttggaaag tcgctgatac tcatcgctga tgcgctccca tatcgcctga accgcttcca
     2401 actcctgagt cgccgtgcca cgacctgcgc ctcgcgtgac cgactcgtac agacgctgag
     2461 cgctgatgta cagtcgtcgc gccctttgaa aggatctcag aacctgaacc gaggacttca
     2521 gaatgtcgcg agcggacacg ccggaattcg catactgccg cagttcattt tcaaagagga
     2581 taatgttatc ggacatcgct tcttcggcat cctgcaactg cgtgacgaca ctttcgtcga
     2641 tcgttgggac attcaggctg ctcagcaatg aggcctgttc ggttttgaat tttgtcaaat
     2701 cggtgccgat cgtattcaac tggtttgcca ggtcggacaa tcgccgattc agatcattca
     2761 ccagattacg gagcaccaga ctggaggcca gtttttcggc catgcgatcg atcaactgag
     2821 ccttctgctc ggtcagagac tgcagatccg tcctcaattc ctcaacgtcg gcggccacgt
     2881 cggcaagatc gccctgaatg atcgcattca gggcttcccg ggcgtcgaga tagacgcttt
     2941 gagcctgtcc gggcgtcaaa acggcagctc tcgacatctc aaaaacttcc ctgattcgac
     3001 cgacctcatc ggacaatcca gccatctctt caccgagggc gatgacccga tcacccaact
     3061 cctgaatcgc cgcctgttcc gcttcgatat tcaccgaacc agcgctacca ccgcgctgac
     3121 tgatgcgacg cagttcctga cgatagccac cgatggcatc actcaatgac gccgcctggc
     3181 gagccaaagc gcgtgcctgt tgcagattgc cctggccggc attgatcata tcggccagct
     3241 ggcgctgcat gcgcccaatc tcagcttctg cggcagaaag agtctgctga tactcagcga
     3301 ctctctgccg aagctcgagc atggctggcg cattagcggc cgttccgtcg ggaatcgccg
     3361 cgttcgcctc gcggatcgcg gtcatgccgt tcgccagcga tgagcgaagg gattcgagac
     3421 gccctggtat ctcacctaaa tcgagcgatt cgatctgccg caactgccgc agcatcgcgc
     3481 caacatcagt tctatacgaa gcattactgc gcagacaatc ggcaatcgcg ctaacgagcc
     3541 cctgatcagg gcgactggag gattcgcgca cgcgccgcat gaagttgttc atgatcatgg
     3601 catcttcatc gagtacgccg atcgcgcgat cgacggcctt gagaagagcg ggcgcgctct
     3661 cacgagttgc gccttccaga ctcgaccgca ggtttcgaat caacgttccc tcgttctcgc
     3721 ggatgttacg gagaatcccc ggaatatcgc caaaatccac actcccaccc atgttctgga
     3781 gttggttgtt ccactgattc cccagctgat tggtatcgat attctgctgc gtcacctgtc
     3841 ccccgcgaat gatgagctgc tgcccctcgc ccacggagaa atgctccccc gagttgcgaa
     3901 tggtgacatc agcctcgccg cgcaacgtcg cgatcgtatc gatctcgacg ccactatcgc
     3961 gtccgcgacc acaggtgatg
//
LOCUS       DAORHD010000309         3977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24696_length_3977_cov_3.593320, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000309 DAORHD010000000
VERSION     DAORHD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24696_length_3977_cov_3.593320"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..460)
                     /locus_tag="PKO06_07785"
     CDS             complement(38..460)
                     /locus_tag="PKO06_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide isomerase family protein"
                     /protein_id="HNV69579.1"
                     /translation="MKLRSLFVLVLCFLLTASLALVASDDWLKNIDDGLKQAKAENKI
                     VMVDFTATWCGWCKKLQTDVFDREEFQKFAAEHLVLVSIDADANRDLVTKYGVEGFPT
                     IVFLNANGDKIHTISGYLPLEGFLSELQEAQKKSNGGK"
     gene            complement(551..1438)
                     /locus_tag="PKO06_07790"
     CDS             complement(551..1438)
                     /locus_tag="PKO06_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HNV69580.1"
                     /translation="MTTNPPLFGRIISLDQLLKPATDEPRKLSLRGRGDCELYFLTPD
                     QHLVTVAPYAWGRGPNRILVEQLPDPTEMLIAEGWRLTDQELRLPTGDVLPIDKSRWH
                     LNSLLEPVKEFPGHLRNALNRLQKRFSLELDTRMQNLAQALKTPLAESVELPLLQVIG
                     FGDGNHPDGDAALCGLLLTGRAISLGKRLKVHWLPRLVPETRRFLHRTPKISASLIRF
                     ALEGRITERQERFFTAMGRDYESAVEAALANLLDGPERGSLAFLTGVKTALEMIRQDM
                     ELVQSRPTPRSTAVRPDKN"
     gene            1666..2436
                     /locus_tag="PKO06_07795"
     CDS             1666..2436
                     /locus_tag="PKO06_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69581.1"
                     /translation="MATRMTNPLRATGSRQIVQRLPWALTLVWLFAVLFVFGCGGGGG
                     SGGGRQILPGENPVGPTTPTTPTTPATSTPIIPTPTPVATPGAYIINGWIDMGYGNYS
                     SAQQKFQTVLGLASSTSAEKADALNGIGWAKGKNYGIAAGANDFIQAGAVEEAIVGLA
                     AAYVQSADPAKIVQAVSLLESIGMGDPLKPFVPVHPSIGVTNAEVHAMLAYAYYWSGN
                     EAGARAQINAARASVDPNAASSVNQIYQSLKQLGLTGI"
     gene            2451..3491
                     /locus_tag="PKO06_07800"
     CDS             2451..3491
                     /locus_tag="PKO06_07800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69582.1"
                     /translation="MLLPSLCRWLLLLCLVCSAEASAQVPQVHEAAKTGDLESLEKML
                     DSRPADVNSTASYGIFPENYQMTPLHWAARTGHLEIARLLLKRQANVNCRTSNGITPL
                     HMAALRGQVAMCDLLLDQHAILDQADDAGMTPLHMAALGGSAAVIERLVSRQAKLETE
                     DRNGDTPLHVASLHGNNAAAYRLLKLKAPVSVRNHQGETPLHEAVATRNLVIVKALLE
                     AGADPNLPDDRLQTSLHLAARNLHLESIEVLLRAGADPQRKDREGFTVLHAAVQKSFM
                     KRMREVLTPQDIDGLVKIVQRLIEAGAELETRSVEGKNARETLGFLLSPSEQARLERM
                     QKSVKKKVGTLR"
     gene            3595..>3977
                     /locus_tag="PKO06_07805"
     CDS             3595..>3977
                     /locus_tag="PKO06_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69583.1"
                     /translation="MAEETFTLYDYFKIVKKRKTVFLVPFFVVVILGLWFTLAQPEVF
                     QVSSSFLVDPPTAYDPPQFVTQLLEKARSRELIDETLKTRGLRMDPEVIMNNITVRAD
                     AGNNSVLRVTLHHLASLALFLLAVGL"
BASE COUNT          873 a         1069 c         1149 g          886 t
ORIGIN      
        1 tgaccggggc ggcgtgttgc tttttatctg agacgaacta cttcccgccg ttggacttct
       61 tttgagcttc ttgcaactcg gacaaaaacc cttcgagtgg cagatatccg gaaatggtat
      121 gaattttatc gccattggca ttcagaaaca caatcgtggg aaaaccttcc acgccgtatt
      181 tcgtaaccag atcgcggttg gcatctgcat cgatggaaac gagcaccaga tgctcggcgg
      241 cgaacttctg aaattcttcg cggtcaaaga cgtcggtctg aagctttttg caccagccgc
      301 accatgtcgc cgtaaagtcg accatgacga tcttgttctc ggccttggcc tgtttgaggc
      361 cgtcatcgat gtttttcagc caatcatcgc tcgcgaccag tgccagactg gcggtcagca
      421 gaaaacacag cacgagaacg aaaagggagc gcaatttcat acggttcctc cgaaatgatt
      481 cgtggcagct gaaactgcca tggccgaatg atacccgaaa atgtgctgcc cgaacagtct
      541 tcggcggaga ttagttcttg tcggggcgaa cggcagttga tcgaggtgtg ggacgcgatt
      601 ggacaagttc catatcctgc ctgatcatct cgagagcggt tttcactccg gtcagaaagg
      661 cgagtgaacc gcgttccggc ccatcgagca ggttcgccag cgcggcttca accgcgcttt
      721 cgtagtcgcg tcccatggcg gtgaaaaaac gttcctgtcg ttcggtgatt cgaccttcga
      781 gggcaaaacg aatcagactg gctgaaatct tcggcgttcg atgcagaaaa cggcgggttt
      841 ccggcacgag tctcggcagc caatggacct tcaaccgctt gcccagagag atcgcgcgac
      901 cggtgagtaa caagccgcag agagccgcat ctccgtccgg gtgattcccg tcgccgaagc
      961 cgatcacttg cagcaaaggc agctcaacag attctgccag aggcgtcttc agggcttgag
     1021 cgagattctg catgcgagtg tcgagttcga gagaaaatcg cttttgcagc cgattcaggg
     1081 cgttgcgtaa atgtccggga aactctttga caggctcgag aagtgagttg agatgccagc
     1141 gtgacttatc gatgggtagg acatcgccgg tgggcaaccg cagttcctga tcggtcagac
     1201 gccagccctc cgcgatcaac atttccgtgg gatcaggcaa ttgttcgacg agaatgcgat
     1261 tgggccctcg cccccaggca taaggcgcga cagtgaccag gtgctgatcg ggcgtcagaa
     1321 aatacagctc gcagtcaccg cgaccgcgca gggagagttt gcgtggttcg tcggtggcag
     1381 gtttcaacaa ctggtcgagc gaaatgattc tgccaaaaag gggcggattc gtcgtcatgc
     1441 cggtatggta tcacactctt tttgtcactc gacacgacga aaaagccttg aggtttttct
     1501 tcttccggcc gatacacagg aaagcctttt ctgtctgtcc ggaaatgcga atgtctcggc
     1561 aactcgagcg tcgctgatct gaacagctgc actggtctgc agaagggcga tgtctgtgaa
     1621 cttcacgatc tgatgcagta acgaagtcgt gttgaggaga tgtgcatggc cacgcgaatg
     1681 acgaatcccc ttcgggcgac cggctcccgg caaattgttc agcgccttcc ctgggcgctc
     1741 accctggtgt ggctctttgc tgtcctgttt gtgttcggct gtggtggcgg tggtggttca
     1801 ggcggtggcc gccagattct tcccggtgaa aacccggtag gaccgaccac tccgacgacc
     1861 ccgaccactc ccgcaacgtc gactcccatc attccaacgc cgactcccgt cgcgacgcct
     1921 ggcgcgtata tcatcaatgg ctggatcgat atgggatacg gaaactattc ttccgcgcag
     1981 cagaaatttc agaccgtgct tggattggcg tcatcgacgt ctgcagaaaa ggctgatgcc
     2041 ttgaacggca tcggctgggc gaagggaaaa aactacggca tcgcggcagg cgcgaacgac
     2101 ttcattcagg ctggtgccgt ggaagaagcg atcgtgggtc ttgcggcagc ctatgtgcag
     2161 tcggctgacc ccgcgaagat cgtgcaggcg gtgtctcttc tcgagagtat tggcatgggt
     2221 gatccactga agccgttcgt cccggttcat ccctccatcg gagtgaccaa cgccgaagtg
     2281 catgcgatgc tggcgtatgc ctactactgg agtggaaacg aagcgggtgc ccgggcgcag
     2341 atcaacgctg cccgtgcctc ggtcgacccg aatgcggcat cttcggtgaa tcagatctac
     2401 caatccttga aacaactggg actgacggga atatgagctt gagccgccga atgctgctgc
     2461 cttctctctg caggtggctg ctgctgctgt gtctggtctg ttcggctgag gcgtcggctc
     2521 aggtgcctca agtacatgag gcggccaaaa ccggagacct tgaaagcctt gaaaaaatgc
     2581 tggattcgcg accggcggac gtgaactcga cggcgagtta cggcattttt cctgagaatt
     2641 accagatgac tcctcttcat tgggcggcgc gcacggggca tctggaaatc gcgcgactgc
     2701 tgttgaaacg ccaggccaac gtcaactgtc gtaccagtaa tggcattacc ccccttcaca
     2761 tggcggcatt gcgcggccag gtggcgatgt gtgatctgtt gctcgatcag cacgccattc
     2821 ttgatcaggc tgatgatgcg ggaatgacgc cccttcacat ggcggctctc ggtgggagcg
     2881 cggcggtgat tgaacggctg gtgtctcgac aagcgaagct cgagacagaa gaccgcaacg
     2941 gcgacacccc cctgcatgtc gccagtctgc atggaaacaa tgctgccgcc taccggctgt
     3001 tgaaactgaa agcgccggtc agcgtgcgga atcatcaagg agagacaccc cttcacgaag
     3061 cggttgccac gcgaaatctt gtgatcgtga aagcattgct cgaggcgggc gctgatccga
     3121 atcttccgga cgatcgtcta cagacctcgc tgcatcttgc cgcgcgcaac cttcacctcg
     3181 agagtatcga ggttctgctg cgtgcggggg ctgatccgca gcgcaaggat cgcgaagggt
     3241 ttaccgtgtt acatgccgcg gtgcagaaaa gtttcatgaa acgcatgcga gaggttctga
     3301 cgccacagga catcgatggg ctggtgaaga tcgtacagcg gttgattgag gcgggggccg
     3361 agctcgagac ccgcagcgtc gagggaaaaa acgcgcgcga aactcttggt tttcttcttt
     3421 ccccttctga acaagcccgt ctggagagaa tgcaaaagag tgtgaagaaa aaagttggta
     3481 cattaaggtg aactgcgtat aattacctgt tgaaattgtt tcacgtgggt caaggtattg
     3541 agaaaaatga cgatagagta gaagacccgt cattctagaa ctgaggtagg aaacgtggcg
     3601 gaagaaacct ttacgctata tgactatttt aagattgtaa aaaagcgaaa aacggtcttt
     3661 ctggttccgt ttttcgtcgt ggtgattctg ggcttgtggt ttaccctggc tcagcctgag
     3721 gtgtttcagg tttcttcctc atttctcgtc gaccccccta cggcttatga ccctcctcag
     3781 ttcgtcaccc agcttctcga aaaagctcga tcacgtgaac tgatcgacga aacgttaaaa
     3841 acacgcgggc tccgcatgga cccggaagtc atcatgaaca acatcaccgt tcgtgccgac
     3901 gccggaaaca acagcgttct tcgcgtcacg ctgcaccacc tcgcctcgct ggcgctgttt
     3961 ctcctcgccg tcgggct
//
LOCUS       DAORHD010000310         3970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24761_length_3970_cov_3.754789, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000310 DAORHD010000000
VERSION     DAORHD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24761_length_3970_cov_3.754789"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /gene="leuS"
                     /locus_tag="PKO06_07810"
     CDS             <1..1456
                     /gene="leuS"
                     /locus_tag="PKO06_07810"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNV69584.1"
                     /translation="TGAVMAVPAHDERDFAFAKKYQLPVREVITPKGQKQGSLSAAYT
                     EYGTLVESGEFSGLSSEQAIEKMATWVESRKLGKKRVNYKFRDWLISRQRYWGAPIPV
                     IHCDTCGTVPVPEKDLPVLLPEIKEFKPGEDGKSPLAGIDSWVNTTCPKCGKPARRET
                     DTMDGFACSSWYFLRFLDPNLNTAAFDRDKAAYWLPVDLYVGGAEHAVMHLLYARFWT
                     KVMKDAGLVPFAEPFAQLRNQGMMLAADGQKMSKSKGNVITPDEMVEKYGADTLRAFI
                     LFLGAFELDVAWSDEGIRGMFRFMNRVYDFVQQHPGSPGTSVGTDAVDLHRWIHKTVK
                     AVTKDIEGFSFNTAIARLMEYVNALQDFAKTSHLSRTDLWIKAIETLLKLLAPIAPFL
                     AEDLWHKTGHTDSVHQQPWPTWDERALIEESVTLPVQVNGKLRDQLKLPVNLTEAQIR
                     EECLKSEKVQKFIEGKEIIKFIYVPKRMASFVVK"
     gene            1608..2369
                     /locus_tag="PKO06_07815"
     CDS             1608..2369
                     /locus_tag="PKO06_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69585.1"
                     /translation="MDRQPELSAETVRICSCQTRNHGQAKFCCGCGRSLVETAKPSVS
                     ENQSERVPDTLSASTKALTTRFSGAELFRLILRTVRLFNRQQPGTQRLTLLIAICGVG
                     LVLSLPLLGFAFRQSDVPHARKPASGPALFQDVPLDHPLYRAWKTLLKTGAPLADRDE
                     SSHPYDPVTWEDWNRVVSHAFQQLVPELEIQQLLALHRAGPMHPQDLKSELMELGRAL
                     EVDRVESYLPAHAIDVPSRLEAFAALSRLFSEAGR"
     gene            2366..3274
                     /locus_tag="PKO06_07820"
     CDS             2366..3274
                     /locus_tag="PKO06_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69586.1"
                     /translation="MNTSHRFWVTLGLVAFLNTPACAGLLTAIVPQAGESESKLIITL
                     TDNDIPTPRFIPEEDCWHIDFPKTESRVQLPTQVFDRGPLRLVRLAQVCDVPPIQRLV
                     CHVQTGTRLKVRRVGSECALIFKRGEETRPRLPGPPDENTSGLISPEMTRNEILIQVR
                     KTQTAPLFLELAARAGVELHFRDRPGAAALQLHGTDPLTLMKDLARHLNMSLTLEDGV
                     WWLSRRDNPLLTIPTTGKISASSLAGLTISQALTRLVGDSASRKLLNRLPRKLRDRPL
                     TASTVTDACPRRWVEQLLEAHGVETR"
     gene            3274..>3970
                     /locus_tag="PKO06_07825"
     CDS             3274..>3970
                     /locus_tag="PKO06_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNV69587.1"
                     /translation="MSHHKRRSLRRRLWFTLSLPLFVIVLMGAIPSHQTVTRLEMAKA
                     VATLLPGHLSPSDADVASLFTDISPAQGSDLRTTLERGLMQGYPDGSFRPHDPVRWAE
                     CLQTWTKLVLVTRPDLAAAGSETPWPESEMAILQRAEFPLPDSVQQRRFAAQVTSDEF
                     DTLLDTTRRLLRIAPPTMTTASPDVEEAQATETATRQSDSPETKQTVQQEIAESLSTP
                     QEPAAGFRLRGKLL"
BASE COUNT          831 a         1217 c         1083 g          839 t
ORIGIN      
        1 caccggagcg gtaatggcgg tgcccgcgca cgatgaacgt gattttgcct tcgccaagaa
       61 gtaccagttg ccagtgcgcg aagtcatcac cccaaaagga cagaagcaag gttcattgtc
      121 tgccgcctac accgaatacg gtacgctcgt cgaatcgggt gagttttccg ggttgtcctc
      181 agagcaggcg atcgagaaga tggccacctg ggtcgagagc cgaaaactcg ggaagaaacg
      241 ggtcaactac aagttccgtg attggctgat ctctcgtcag cgctactggg gtgccccgat
      301 tccggtcatt cactgcgaca cctgcggaac agtacctgtt cccgaaaaag atcttccggt
      361 tctgttgccg gaaatcaaag agttcaaacc cggcgaagat ggcaagtctc ccctggcggg
      421 catcgactcc tgggtcaata cgacctgtcc aaagtgcgga aaaccagccc gccgtgaaac
      481 cgataccatg gacggctttg cctgttcttc ctggtacttt ctgcgttttc tcgacccgaa
      541 tctcaacacc gcagccttcg atcgcgacaa ggcggcatac tggctgcccg tcgacctgta
      601 tgtcggtggc gctgaacacg ccgtcatgca tctgctgtat gcccgcttct ggaccaaagt
      661 catgaaagat gcgggactgg tgccatttgc cgagcctttt gcccaactgc gtaaccaggg
      721 catgatgctc gcggccgacg gtcagaagat gagcaagtcg aaaggcaatg tcatcactcc
      781 cgacgagatg gtcgagaaat acggagccga taccctgcga gcctttatcc tctttctcgg
      841 cgcgtttgaa ctggatgtcg cctggtcaga tgaaggcatt cgcggcatgt tccgcttcat
      901 gaatcgggtc tacgacttcg tgcagcaaca tcccggcagt ccaggcacgt cggtcggaac
      961 cgatgccgtc gacctgcacc gctggattca caaaaccgtg aaagccgtga cgaaggatat
     1021 cgaaggattc agttttaaca ccgcgatcgc tcgcctgatg gagtatgtga acgccctgca
     1081 ggacttcgca aagaccagcc acctcagccg caccgatctg tggatcaaag cgatcgagac
     1141 cctgctcaaa ctgctggcac ctatcgcccc gttcctcgcc gaagatctct ggcacaaaac
     1201 cgggcacacc gattccgtgc atcagcagcc ctggccgacc tgggacgaac gggctttgat
     1261 cgaagagtcg gtcaccctgc cggttcaggt gaacggaaag ctgcgtgatc agctgaagtt
     1321 gcccgtcaat ctcaccgaag cccagattcg cgaagagtgc ctgaagagtg aaaaggtgca
     1381 gaaattcatc gagggcaaag aaatcatcaa gttcatctat gtgcccaagc gcatggccag
     1441 tttcgtcgtg aaatagacag acaccacctt tcgccgcatg acgcccgcgt ttcacgcggg
     1501 cgttctcact tttgtctgca atcaggttca gcgttcgtca aaatctgccg ataagcagaa
     1561 gtggggtatc aggtctcgac ctctggaaag gatgcttcac gggaaacatg gatcgccagc
     1621 cggaactttc cgccgaaacc gttcgtatct gctcgtgcca gacgcggaat cacgggcagg
     1681 ccaaattctg ctgcggttgc ggtcgttctc tggtcgagac ggcaaagccg tctgtttctg
     1741 aaaaccaatc cgaacgtgtg cctgacacgc tttctgcctc gaccaaggct ctgacgactc
     1801 gtttttctgg agccgagctg tttcgtctga ttctgcgcac ggtccgactg ttcaatcgac
     1861 agcagccggg aactcagcgt ctgaccctgc tgatcgcgat atgtggcgtc ggcctggttc
     1921 tctctcttcc gctgctgggt tttgcctttc gtcagtcaga cgttcctcac gcgcgcaagc
     1981 cggcttccgg tcccgcttta ttccaggatg ttcccctcga ccacccgctg tatcgcgcct
     2041 ggaagaccct gctcaaaacc ggcgcgccgt tggctgaccg agatgaatcg tcacacccct
     2101 atgacccggt aacctgggag gattggaatc gtgtggtcag tcatgccttt cagcaactgg
     2161 tgccggaact cgagattcag caactgctcg ccctgcatcg tgccgggcca atgcaccccc
     2221 aggacctgaa atctgagttg atggaactgg gacgcgcgct ggaggttgat cgagtcgaga
     2281 gctatctccc cgctcacgcc atcgatgttc ccagtcgact cgaggcattc gccgcattgt
     2341 cacgcctgtt ctcggaagcc ggcagatgaa tacctctcat cgtttctggg taactctcgg
     2401 tctcgtcgct ttcctgaaca cccctgcgtg cgccggactg ctgacagcca ttgttccgca
     2461 agcaggagag tccgagtcga aactcatcat tactctcact gacaacgaca ttcctacccc
     2521 gcgattcatt ccagaagaag attgctggca catcgacttt cccaagacgg aatcgcgcgt
     2581 ccagctaccc acgcaggttt tcgaccgagg tccactgcga ctggttcgtc tggctcaggt
     2641 gtgtgacgtg ccccccattc aacgcctcgt ctgccatgtt cagacgggaa cacgtttgaa
     2701 agttcggcgg gtcggctctg aatgcgccct gatcttcaag cgcggcgagg aaacgcgtcc
     2761 acgccttccc ggtcctccgg acgagaacac atcagggctg atctcacccg agatgacgcg
     2821 caacgagatt ctgattcagg tgcgaaaaac ccagactgcg cccctgtttc tcgaactggc
     2881 ggcccgagcc ggggtagaac tgcattttcg cgaccgaccg ggggcggccg cccttcagct
     2941 gcacggaaca gaccccctga ctctgatgaa ggatctcgcc cgccatctga acatgtctct
     3001 cacgctcgaa gatggtgtct ggtggctcag tcgccgcgac aatccacttc tcaccattcc
     3061 gacgaccggc aaaatctccg ccagttctct tgcaggactg acgatatctc aggccctgac
     3121 acgtctggtg ggagattccg cgagtcgcaa gctgctcaat cgactgccgc ggaaactgcg
     3181 agaccggcca ctgacggctt cgacggtgac agacgcgtgt cctcgccgct gggttgaaca
     3241 gcttctggaa gctcacggag tggaaaccag gtaatgagtc accacaaacg acgttctctg
     3301 cgccggcgac tgtggttcac attgtctctg cctttgttcg tgatcgtgct catgggggcc
     3361 attccttcgc atcagacagt cactcgtctg gaaatggcca aagccgtggc cactctcctt
     3421 cccgggcatc tgtcaccatc agacgctgat gtcgccagtc tcttcacgga tatttctccc
     3481 gcccagggca gtgacctgcg taccactctg gaacgcgggt taatgcaggg gtatccagat
     3541 ggctcgttcc gaccgcatga tccggtgcgg tgggccgaat gtctgcaaac ctggaccaaa
     3601 ctggtgcttg tcacgcgacc ggacttggcg gcggccggga gtgaaacccc gtggcctgag
     3661 agcgaaatgg cgattctgca gcgggcggag tttccgcttc ccgattccgt gcagcagagg
     3721 cggttcgcgg ctcaagtcac ctcggacgaa tttgatactc tgctcgacac cactcgacgc
     3781 ctgctgcgca tcgctcctcc cacgatgacc acagcttcac cagacgttga agaagcacag
     3841 gcgacggaaa ccgccacgcg tcaatccgac tcgcctgaaa ccaagcaaac cgtgcaacag
     3901 gagattgccg agtctttgtc caccccccag gagccagctg ccggatttcg tcttcgcggc
     3961 aaactgctgg
//
LOCUS       DAORHD010000311         3968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24776_length_3968_cov_3.001022, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000311 DAORHD010000000
VERSION     DAORHD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24776_length_3968_cov_3.001022"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="PKO06_07830"
     CDS             complement(<1..1060)
                     /locus_tag="PKO06_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69588.1"
                     /translation="MTDQSVPVTGCPSEPRTVSEDSWQAFLESLYAPLPSLREMAVLE
                     LPRWAELGRDVSEVLALSINDEVPLVVRAAVMVAGRLRVPRRSVVEALERRLVTSDEL
                     LRRTVVTALGRIGSPAFQVLARLLNDRDPFIAEFVQTTLAIEGEPAVSVLLPLLSDLR
                     LRQFAAAVLVRIGPPAIAHLMPLLNHADSEVRFLVTDTLEQIGPVVLPTLLDALRQSG
                     AEREGAAAALPRFGIDAIPGLIELLSDANVHHRCSAAAALVQHGVRAVPFLVKALEHK
                     NTGVAWLAAKSLGQIGPSAIPDMAIHLGRTARSVRWILADVLRQMGSDAALQLEKELS
                     NPDPLVREAVAHILGDIGE"
     gene            complement(1079..2119)
                     /locus_tag="PKO06_07835"
     CDS             complement(1079..2119)
                     /locus_tag="PKO06_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNV69589.1"
                     /translation="MTSTNQWYLAVGGQPQGPMSFEAVVAKLKRGAATKDTLAFAQGV
                     TQTWATLEEIPEFQGHLFTSLEFPTVPTGTAQTKGQADEIDFDIYGEDMQFVEIELDP
                     GESAIAEAGAMMYMDSVIEMETIFGDGSAKSAEETSGWWGAIKSAGKRILTGESLFMT
                     MFTHKGTSGKAKVAFAAPYPGKIAAVNLRDIGGTLICQKEAFLAGARGVAVGMHLQKK
                     LGVGFFGGEGFIMQKLEGDGMVFCHAGGTMMTRQLEAGQTVKVDTGCVVAYTPSVNFD
                     IQMVKGVKSMLFGGEGLFFATLTGPGRVWLQSIPFSRLATRIFAASRQQGGGKEEGSV
                     LNTVFNLTGGKS"
     gene            complement(2267..2539)
                     /locus_tag="PKO06_07840"
     CDS             complement(2267..2539)
                     /locus_tag="PKO06_07840"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNV69590.1"
                     /translation="MGRRNIIEKIQHGQYVFTQHAYQQMLARDIYIRQIPDAIQQGRV
                     VKRWNERDGEKLAIVGPRFNGDYIKVLVKDADTPRIITVCYPYEEI"
     gene            complement(2634..>3968)
                     /locus_tag="PKO06_07845"
     CDS             complement(2634..>3968)
                     /locus_tag="PKO06_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69591.1"
                     /translation="EIEAKIRTLQGVQNLYSSIGEGSLAGVNDAQIFVQLKDLKERDF
                     SMFDSMTAARKILSEYPRLRPAVQASGGISGAKNYDTQMYIRGPDFKQINTWVADIMT
                     RMRQNPLFANVDSSFIEGKPEVQVVFDRERAHRLGVRMEQVAAGLRTLVGGKDQITRF
                     KEGEELYEVRVRLRPEDRVNPEAIAGLLFPTTNGTMVRLDAVASIRTGAGPAQIDRQD
                     RQRAITVYANLAPKAGLGDANDFLLKCAKAQNMPSAYTSGFTGRGREMERTKRGFGIA
                     FLLSAIFMYMILASQFESFLHPVTIMLSLPLSVPFAILSLLLTDSSLNVFSGLGVFML
                     FGIVKKNAILQIDYTNTLREKGLNRDQAVVEANHARLRPILMTTITLVIGMLPMALGT
                     GPGAAVRATLATVIVGGQSLCLLITLLLTPVAYTLFDDVVVWLQQHMPRLER"
BASE COUNT          820 a         1145 c         1218 g          785 t
ORIGIN      
        1 actccccgat gtccccgaga atgtgcgcga cggcttcccg taccagaggg tccgggttcg
       61 acagctcttt ctcaagctgc agggcggcat ctgaacccat ctgtcgcaga acgtccgcca
      121 gaatccagcg tactgagcgt gcggtacgac cgagatgaat tgccatgtcg ggaatggccg
      181 aggggccgat ctgtcccagt gacttcgccg ccaaccaggc gactcccgtg ttcttgtgtt
      241 cgagcgcttt gacgagaaaa ggtacggcac gcacaccgtg ttgcaccaga gctgcggcgg
      301 cagagcagcg atgatgcacg ttggcgtcac tcagcagctc gatgagcccg ggaatcgcgt
      361 cgattccaaa gcgtggcaag gcggcggcgg caccttctcg ttccgcgcct gattgtcgca
      421 gggcgtcgag cagggtcggc aggactacgg gaccgatctg ctcgagggtg tctgtgacca
      481 gaaaacggac ttcggaatcg gcatggttca agagaggcat caaatgagct attgccggag
      541 gtccgattcg gacaagaacc gcagcggcga attgtcgcag acgcagatcg ctcagcaacg
      601 ggaggagcac cgaaaccgcg ggctctccct cgatggccag agttgtctgg acgaactcgg
      661 caataaacgg gtcgcgatcg ttcagaaggc gggcaagcac ctgaaaggcg ggcgaaccga
      721 tgcggccgag ggccgtgacg acggtgcggc gaagcagttc gtcagaggtg acgagacgac
      781 gctcgagggc ttcgacgaca ctgcgacggg gaacgcgtaa tcgcccggcc accatgacgg
      841 ccgcccggac gaccagaggt acctcgtcat tgatcgacag cgccagaact tcactgacat
      901 cgcgtcctag ttccgcccag cgtggaagct ccaggaccgc catttcgcgc agggagggaa
      961 ggggcgcata cagggattcg aggaatgcct gccagctgtc ctccgaaacc gttcggggtt
     1021 ccgaaggaca gccggtcacg ggcacggatt gatcggtcat ggtctcggca gaagccgatc
     1081 agctcttgcc accggtcaga ttgaagacgg tgttcagaac agaaccttct tccttgccgc
     1141 cgccctgctg gcgtgacgcg gcaaagatgc gggtggcgag gcgcgagaat ggaatcgact
     1201 gcagccacac gcggccggga ccggtcagag tcgcgaagaa cagaccttcg ccgccgaaca
     1261 gcatcgactt gacccctttg accatctgaa tgtcgaagtt gacactgggg gtataggcga
     1321 ccacgcagcc ggtatcgact ttcaccgtct ggcctgcttc gagctggcgg gtcatcatcg
     1381 tgccgcccgc gtgacagaag accatgccgt cgccctcgag cttctgcatg atgaagcctt
     1441 cgccgccaaa gaatccgaca cccagtttct tctgcaggtg catgcctacg gcaacgccgc
     1501 gggctccagc caggaaggct tctttctggc agatcagcgt gccgccgatg tcacgcaggt
     1561 tgaccgccgc aatctttccg gggtagggag ctgcaaaagc caccttggct ttgcccgagg
     1621 tgcccttgtg ggtaaacatg gtcatgaaca gactttcacc ggtgagaatg cgtttgccgg
     1681 cactcttgat cgcaccccac cagccactgg tctcttctgc tgacttggcg ctgccgtcgc
     1741 cgaaaatggt ttccatttcg atgacgctgt ccatgtacat catggcaccg gcttcggcga
     1801 tcgccgactc gcccggatcg agctcaatct cgacgaactg catgtcttcg ccgtagatat
     1861 cgaaatcgat ttcatcggcc tggcctttgg tctgtgccgt accggttggg acggttggaa
     1921 attcaagact ggtgaagaga tgcccctgga attccggtat ttcttcgagg gttgcccagg
     1981 tctgagtgac accctgggcg aaagccaacg tgtctttcgt tgccgcgcct cgcttcagtt
     2041 tggccaccac ggcttcaaaa ctcatcggac cctgcggctg tccgcctacc gccagatacc
     2101 attgattcgt cgatgtcata ctctcactcc tcacatgaaa tggaactgcg ccgtcatcat
     2161 agcacaaaag cggtcgtgtc gcggcactcg tggagaaacg gcgtgacccg ctgcgaaaga
     2221 gggggcagag gagagcgtgt cggctgcggt gttgcagaag accgagttag atctcttcgt
     2281 aggggtaaca gacggtgatg atgcgcggag tgtcggcgtc tttcaccagg actttgatat
     2341 agtcgccgtt gaaacggggc ccgacgatgg cgagtttttc gccatcacgt tcgttccagc
     2401 gcttgacgac gcggccctgt tgaatggcat cgggaatctg tcggatgtag atgtcgcggg
     2461 ccagcatctg ctgataggcg tgctgcgtga acacgtactg tccatgctga atcttttcaa
     2521 tgatgtttcg tcggcccatg gggcacctcc ccggtggtca caaaatgtca ctgaccggaa
     2581 aagtgtagca aaccccctcg tcgccgacaa gtgcgagttg ccggccggcc tggtcagcgt
     2641 tccaggcgcg gcatgtgctg ttgcagccag acgacgacat cgtcgaacaa cgtataggct
     2701 accggagtca gcaggagcgt gatcaacaga cagagcgact gaccaccgac gatgaccgtg
     2761 gcgagggtgg cacgaaccgc tgcaccaggc ccagtcccca gagccatggg cagcatgccg
     2821 atgaccaggg tgatggtagt catgaggatc gggcgcaggc gggcatgatt cgcctcaacg
     2881 acggcctgat cacgattcaa acccttctcg cgcagcgtgt tcgtgtaatc gatctggaga
     2941 atggcgttct tcttcacgat gccgaagagc atgaagactc cgaggccgga aaacacgttc
     3001 aatgagctgt ccgtcagcag cagagacaga atggcgaacg gcacggaaag gggcagggaa
     3061 agcatgatgg tgacggggtg cagaaagctt tcaaactgcg atgccagaat catatacatg
     3121 aaaatggcgc tgagcagaaa ggcgattccg aagccccgct tcgtgcgttc catctcgcgc
     3181 ccgcgaccgg tgaagccact ggtgtaggct gagggcatgt tctgagcctt ggcgcacttc
     3241 aacagaaagt cgttggcgtc accaagaccg gctttggggg caaggttggc atacaccgtg
     3301 atcgcgcgct gacggtcctg gcggtcgatc tgtgccggtc cggcacccgt tctgatcgaa
     3361 gcgacggcat cgagacgaac catcgttccg ttcgtggtcg gaaagagcag tccggcgatg
     3421 gcctcggggt tcacgcgatc ttcggggcgc agtctgaccc gcacttcata caactcctct
     3481 ccttccttga aacgggtgat ctggtctttg ccgccgacaa gagtacggag ccctgcggca
     3541 acttgctcca tgcgcactcc cagcctgtga gcccgttcac ggtcgaatac gacttgtact
     3601 tcaggttttc cttcgatgaa tgacgagtcg acgttggcga acagcgggtt ttgacgcatg
     3661 cgtgtcatga tgtcggcgac ccaggtgttg atctgcttga aatcagggcc acggatgtac
     3721 atctgggtgt cgtagttctt ggccccggag atgccccctg acgcctgaac cgccggtcgc
     3781 aagcgcggat actcggagag aatcttgcgc gcggccgtca tcgaatcgaa catcgagaaa
     3841 tcacgctctt tcagatcttt gagctgtacg aagatctggg catcgttgac cccggcaagg
     3901 gagccttctc cgatcgaact gtacaagttc tgcaccccct gcagcgtgcg gatcttggcc
     3961 tcgatctc
//
LOCUS       DAORHD010000312         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24781_length_3967_cov_3.697597, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000312 DAORHD010000000
VERSION     DAORHD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24781_length_3967_cov_3.697597"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="PKO06_07850"
     CDS             complement(<1..1117)
                     /locus_tag="PKO06_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV69592.1"
                     /translation="MTWSLKRKILVPTIVLIVVTMSISIARTYMLATDALSRNAFAQL
                     NSMTHATVDLVDIWAEDLKTLVIAITERPEFAALLRNDTEATRDQCCRILKSLQEIAP
                     QFAYFHLYNAAGEARASSLVDSIGKVKVPDRAYFLEAMKGNVHFSEVYLSRTTGKPSC
                     AVAAPIRYEGKVIGVLAGIPNIAKFCEELVDGAKVFDTGMISLFDVNGVVVAHKDRAL
                     ILKQDLRQLDHGKKLLERKSGDIRYVLNGREITAMLAPCRAVSWTVLAEAPTSEVLAD
                     ANRLAKNNLFLFVVGLVAIVVLLLAITHSVVGPILRITDGLDQGADHVAQASAEVASS
                     SQTLASGATQQAASLEQTSAALTSLADHTRKNANNADQ"
     gene            1266..2033
                     /locus_tag="PKO06_07855"
     CDS             1266..2033
                     /locus_tag="PKO06_07855"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV69593.1"
                     /translation="MKIRWCLLAVAAVFLFGQNLLAMSADEVLQAVEDRYVGKTSKAE
                     TTMKLIDAEDKERNRTMLVYRRKVDNANKDNFIHFLNPPDIKDTTYLVNEKNREKQKW
                     IYLSAFKNTRKILADDFGSAFVSSDFTYEDMEDIHASDYTCSDLKEETLDGEAVYSIK
                     CQKKDGKTSYAHTIMKVSKEKMVPLKALMFDKGDPSKQIKEMTAKSLQKIQEIWTPME
                     VTMKDLRKGTSTVLAIKKIEYEVPLAEDVFSQRNMQK"
     gene            2154..3362
                     /locus_tag="PKO06_07860"
     CDS             2154..3362
                     /locus_tag="PKO06_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69594.1"
                     /translation="MFVSNSVRADDGEKPVVRPNFSGWHNWKYNKFSSYDQLRLTLDR
                     EFAKGQKFLVSVNGLRYQEDDNKAWNTWVGEAYYKFKRGPFDVKFGQLVETLGSGDKV
                     SFVDKLNSRRYYAGLANDYNRDKKEVPCFKTTYYINKRMSFDVHYLPVFQASELPSIY
                     SKWASEFQKSLAVSILKGGTLAQEDETSLEPQYHLAFNSSFKKYELRYHYFSFKERLP
                     IIDQVNEGSFNQMYPLDETWAVDGNITFSKEFLMRFELAYTRNKRFSAFQSARIGRAF
                     ASDMYNMLLGTDKTYRNNLYLNVQTLISKVAKLPSKTPFQLAETEYAATLQLRKGFKS
                     ERLFIEFNGVNNFTTGEYLLTPNISYQSSDYFKALAGVHLNGRSTYALGPIGQFNKNS
                     TLFLETQVSF"
     gene            3362..>3967
                     /locus_tag="PKO06_07865"
     CDS             3362..>3967
                     /locus_tag="PKO06_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNV69595.1"
                     /translation="MSARPLALLVVGFLICLCCGPITLALETDQATLRFSHYTHHKQK
                     VACVKCHPISLPERSPEPSELPPGWAPLKPSRVADRPAEPAGGGAGGKDSENPFGRPP
                     EKLCLTCHGETRTKSDCGLCHLGKPGPTVRPRQRLMKGVTFDHTKHNTYSCQVCHTAI
                     NNWASLNGSQIDTRMPACLVCHNGVKAKKTCKMCHKPTPYPK"
BASE COUNT          991 a         1139 c         1010 g          827 t
ORIGIN      
        1 cctgatcagc gttgttcgca ttcttccgcg tgtgatcggc caggctggtc aacgccgccg
       61 atgtctgttc gagcgaagcc gcctgctgcg tcgcaccact cgccagagtc tgactggaag
      121 acgccacctc tgcggaggcc tgcgcgacat gatctgcacc ctgatccagg ccgtcggtaa
      181 tgcgaaggat cggcccgacg acggagtgag tgatcgccag cagcagaaca acaatggcaa
      241 ccaacccgac cacaaaaaga aacagattgt tcttcgccag cctgttggca tcggcaagta
      301 cttcgctggt gggcgcttcc gccaggacag tccaggaaac agcccgacat ggagctaaca
      361 tcgcggtaat ctcgcgtccg ttcaacacat agcggatgtc tcctgacttg cgctccagca
      421 gcttctttcc gtgatcgagt tgtcgcagat cctgtttcag aatcaatgct cggtctttgt
      481 gagccacgac caccccattc acgtcaaaca ggctgatcat gccggtatca aagactttcg
      541 ccccatcgac cagctcttcg cagaacttgg caatattcgg gattccggcc aagacgccga
      601 tcaccttgcc ctcgtatctg atgggggcag caaccgcaca cgagggtttc cccgtggttc
      661 gcgacaggta cacttcggaa aagtgtacat ttcccttcat ggcttcgaga aaatacgccc
      721 ggtccggcac cttcaccttg ccgatggaat ccaccagcga agaagctcgc gcttcccccg
      781 cagcattgta cagatggaaa taggcgaact gcggtgcgat ttcctgcaac gatttcagaa
      841 tccgacagca ttggtcacgg gtcgcttctg tatcatttcg gagcagagcg gcgaactcgg
      901 gtcgctccgt aatggcaatg acgagcgttt tcaagtcttc ggcccagata tcaaccaggt
      961 cgaccgttgc atgtgtcatg ctgttgagct gagcaaaggc gtttcgggaa agagcatccg
     1021 tggccaacat atacgtgcga gcaatggaaa tggacatcgt cacgacgatc aggacgatgg
     1081 tgggaaccag gatcttcctc ttcaagctcc aggtcataac agacctcctc tcaaacggta
     1141 tctacacaca ttctagcacg acagccccac ctgctaaatc ctgtttccat cgggcacgtt
     1201 attccgggta tactacgcgt gtctctgttc tttcctgcga gtatcatcac ctaaaggagc
     1261 tgatcatgaa gatccgttgg tgcctgttgg cggttgccgc cgtttttctg ttcggacaaa
     1321 accttttagc catgagtgcc gatgaagtat tacaggcagt cgaagatcgt tatgtcggca
     1381 agacctcgaa ggccgaaacc acgatgaaac tcatcgacgc cgaagataag gagcgcaacc
     1441 ggacgatgct cgtctatcgc cggaaggtag ataacgcgaa caaagacaac ttcattcact
     1501 ttctgaaccc gcccgacatc aaggatacga cctatctcgt caacgagaaa aaccgcgaga
     1561 agcagaagtg gatctacctt tccgcgttca agaacacgcg caagattctt gccgatgatt
     1621 tcggttctgc cttcgtttca agtgacttca cctatgagga catggaagac attcatgcct
     1681 ctgactacac ctgctctgac ctcaaggaag aaacgctgga cggagaggcc gtctactcga
     1741 tcaagtgcca gaagaaagat ggcaagacca gttacgccca cacgatcatg aaggtttcga
     1801 aagagaaaat ggttcctctc aaggccctca tgttcgacaa aggcgacccc tcgaaacaga
     1861 ttaaggaaat gactgccaag agtctgcaga agattcaaga gatctggact ccaatggaag
     1921 tcacgatgaa agatctgcgc aagggaacct cgacggttct ggctatcaag aagatcgagt
     1981 atgaggtgcc gctcgccgaa gacgtctttt cccagaggaa catgcagaaa tgaactagag
     2041 gttgcatgcc cggccggaac gatacctttc gccacgcgaa tcacacatgc cgcagaccct
     2101 ctttattagg gtttgcggca tgtttcgttc tcggactctt gtgtggaaca ttcgtgttcg
     2161 tttcgaactc agtacgagcc gacgatggag agaaacctgt tgtcaggccg aatttcagtg
     2221 gctggcataa ctggaaatac aacaaattct cgtcatatga tcaactgcgc ctgacactcg
     2281 accgcgagtt cgccaagggg cagaaatttc tcgtcagcgt caacggtctg cggtatcagg
     2341 aagacgacaa caaagcctgg aatacctggg tcggagaggc atactacaag ttcaaacgcg
     2401 gcccgttcga tgtcaaattc ggacaactgg tcgagaccct ggggagtggt gacaaggtct
     2461 cattcgtcga taaactcaac tcacgccggt attatgccgg tctggccaac gactataacc
     2521 gcgacaagaa agaagttccg tgcttcaaga ccacgtatta catcaacaaa cgcatgagct
     2581 tcgacgtgca ctacctgccc gtgtttcagg ccagcgaact gccgagcatc tacagcaagt
     2641 gggcatcgga atttcagaag agtctggcag tgagcattct caaaggcggc acgctggccc
     2701 aggaagacga aaccagtctc gaaccgcaat atcatctcgc gttcaattcc agcttcaaaa
     2761 agtacgagtt acgctaccat tacttttcgt tcaaggaacg actccccatc atcgaccagg
     2821 tcaacgaggg cagcttcaac cagatgtatc cgcttgatga gacgtgggcc gtcgacggca
     2881 acatcacgtt ctcgaaagag tttctgatgc gattcgagct ggcgtatacc cgaaacaagc
     2941 gtttttcggc atttcaaagc gcgcgaatcg gtcgcgcgtt tgccagcgac atgtataata
     3001 tgttacttgg aaccgacaaa acctaccgga acaatctgta tctgaacgtg caaaccctca
     3061 tctccaaagt tgccaagctg ccgtcgaaaa cgccgttcca actggcggaa acggagtatg
     3121 cggccacgct gcaactgcgc aagggtttca agagcgagcg actgttcatc gagttcaacg
     3181 gggtaaacaa cttcaccacc ggagaatatc tgctgacgcc gaatatctcg tatcagagct
     3241 cggactattt caaagctctg gccggggtac acctgaacgg tcgttcgacc tacgcactcg
     3301 gccccatcgg ccagttcaat aaaaacagca cgttgtttct ggaaacccag gtgtcgttct
     3361 aatgtcggcc cggccgctcg cgctgctggt tgtcggcttt ctcatctgcc tctgctgcgg
     3421 ccccatcacg ctggccctcg aaaccgatca ggcaacgctg cgattttccc actacacgca
     3481 tcacaagcaa aaagtcgcct gcgtcaaatg ccacccgatc tcgttgcccg agcgcagccc
     3541 cgaaccctcg gaactgccgc cggggtgggc tccactcaaa ccctctcggg ttgccgatcg
     3601 gccggcagaa ccagccggtg gcggagcggg aggcaaggat tcagaaaacc cgtttggccg
     3661 gccgcccgaa aaactctgcc tgacctgcca cggagaaacg cgcaccaaat ctgactgcgg
     3721 cctctgccat ttgggcaagc ccggcccgac cgtgcggccc cgtcagcggc tgatgaaagg
     3781 cgtcacgttc gaccacacca aacacaacac ctactcgtgt caggtgtgcc acaccgccat
     3841 caacaactgg gcttcactga acggcagcca gatcgatacg cgcatgcctg cgtgcctcgt
     3901 gtgtcacaat ggtgtcaaag ccaagaagac gtgcaaaatg tgtcataagc cgacgcccta
     3961 tccgaag
//
LOCUS       DAORHD010000313         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24794_length_3965_cov_3.044246, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000313 DAORHD010000000
VERSION     DAORHD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24794_length_3965_cov_3.044246"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="rplA"
                     /locus_tag="PKO06_07870"
     CDS             <1..600
                     /gene="rplA"
                     /locus_tag="PKO06_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNV69596.1"
                     /translation="ETVDIAVRTGLDPKYADQQLRGSVVLPHGVGKTCRVVVFAAGEK
                     AKEAEAAGADFVGAQDLVTKIEGGWTEFDAAVATPDMMKFVGKLGRILGPRNLMPNPK
                     VGTVTTDIGKVVRELKSGKVQYRVEKAGIIHSVLGKASFGPQKLQDNFMTFLQAVIKA
                     KPSGAKGQYLKNCTISSTMGPGFDLDINAARATVEKTAV"
     gene            794..1330
                     /gene="rplJ"
                     /locus_tag="PKO06_07875"
     CDS             794..1330
                     /gene="rplJ"
                     /locus_tag="PKO06_07875"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNV69597.1"
                     /translation="MNQQKRQMKAAYVDEFQKKMEKAKVVVLAACEGVTVDEMTRLRR
                     EIRKLGDEARVVKNTMLRRACQNLKHENVLPHLTGSTLMTFGYHDPVAPVKALFDFAQ
                     KASKLKFKAGLLGDQLLSVQELENLSKLPGREQLLSMLLSCMQGPMRNMVGVLSGVTR
                     KFVYALNAIKEKKEKAAA"
     gene            1404..1778
                     /gene="rplL"
                     /locus_tag="PKO06_07880"
     CDS             1404..1778
                     /gene="rplL"
                     /locus_tag="PKO06_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNV69598.1"
                     /translation="MTKEQIMEAIEKMSVLELSELVKALEDKFGVTAAAPMAMAMPMA
                     GAAAGAAAAEKTEFDVVLENAGANKINVIKVVRELTGLGLKEAKDLVDGAPKTVKEGV
                     AKADADAMKKKLEEAGAKVALK"
     gene            complement(1771..>3965)
                     /locus_tag="PKO06_07885"
     CDS             complement(1771..>3965)
                     /locus_tag="PKO06_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69599.1"
                     /translation="GLPNLAFLREALTEGFIPFVDPGRGGGMPFLFDPQTCAWYPPAW
                     IAALFEPATGFRLFQFSHLLWLGLGFALFLHSQTGSRTAAFFAGITAIGAVPHLHALE
                     WMPITAGTAWVPWIFLALAGGRIQLWLTASCLSIYSGHAYLWVMTPVLLIAGYQIVPS
                     EWKKTVRLVLFLVPLIGASILLSYVVMSVEFMSHTVDSSHTIEVTSIAPRHLLGFLSP
                     RSLRDFIFGDRTGRVVQIQQWGAFGWSRQCYLGLLPLILTLISFTRWSPIHRMGICLM
                     AGGILLSMILPNLASVFPDVFRHMHHPASFIQLTVWGLLTLGSCGLASLRRAEPSFPR
                     IAPLVILLLGSCGYVIHQYLAERSLDGFGSPLWMFSFQNSQQAYAFGIAITSLLFLLS
                     CIHQVSYRYLILTALIGFQCADLYENSRQTVPLSIPAAGRPTALTHGLASGSKVLVSP
                     GLQYAVLNIPGQPNPRFMQNAYDFVEQTSVPLIGREHGIHHLADYNPPFQHPGRERWI
                     RHIFTRNPVHRDQLLRLSGVTRVLTDQVQWNVSTPARLFPREYCLPAIYSFDIDNSQT
                     ASLVASYSINIFWHANWDKQAIASFPAVDLRWHGATGFLSLPADTTGYTHLYVPVLPL
                     IGWQCHQADRSLSIIRDPDFGIFIEIPPSTSGTLTLSYRPEIWFHVFLPFLLAPILLL
                     SQFRAATPANEPEQTSSTTNNPASSNARKKNPPGSEPGGLCSNESKLT"
BASE COUNT          998 a         1011 c         1218 g          738 t
ORIGIN      
        1 gaaaccgtcg acatcgccgt ccgcaccggc ctcgatccga agtatgccga tcagcagctg
       61 cgtggcagcg ttgtgctgcc tcatggcgta ggcaaaacct gccgtgtcgt ggtttttgcc
      121 gctggcgaga aggccaaaga ggccgaagct gctggagcag acttcgttgg tgcccaggac
      181 ctggttacca agatcgaagg aggctggacg gagttcgatg ccgccgtggc gacccccgac
      241 atgatgaagt tcgtcggaaa actcggccgc atcctgggtc cccgcaacct gatgcccaac
      301 ccaaaggttg gcacggtgac cacggatatt ggcaaagtgg ttcgcgaact caaatcgggt
      361 aaggtgcagt accgcgttga aaaggccggt atcatccatt cggttctggg aaaagcatcc
      421 ttcggtccgc agaagcttca ggacaacttc atgaccttcc tgcaggcagt catcaaggcg
      481 aagccgtcag gagccaaagg tcagtatctg aagaactgca ccatcagttc caccatgggc
      541 ccggggttcg acctcgacat caacgcggcc cgcgctacgg ttgaaaagac cgcagtgtaa
      601 cgttctgtcc tcgtcctgtt cgtagattcg aaaacgtttt cggtcgaaga tcgcaggtcc
      661 tgtcggataa tcggcttacg ggccggcctg ccgagatcga aactggtgaa gtccatcaaa
      721 ggcttccggt ttcatcgacc gggagccttt tcttctagcg tctgaactgt aacaagcaag
      781 gaggtgaaac tacgtgaatc aacagaaacg gcaaatgaaa gccgcctacg tcgatgaatt
      841 ccagaagaaa atggaaaagg caaaggtggt cgttctcgcc gcgtgtgaag gggtgaccgt
      901 cgatgagatg acccgccttc gccgcgagat ccgcaagctg ggtgatgaag cccgggtggt
      961 gaagaacacc atgctgcgcc gcgcctgtca gaatctgaag catgaaaacg tgcttccgca
     1021 tctgaccggc tcgaccctga tgaccttcgg atatcatgat ccggtggccc cagtgaaggc
     1081 gctcttcgac tttgcccaga aggcttcgaa gctcaagttc aaggccggtc tcctcggtga
     1141 ccagctgctg agcgttcagg aactcgagaa tctgtccaag ctgcctggtc gtgaacaact
     1201 gctttccatg ctgctctcct gcatgcaggg tccgatgcgc aacatggtcg gcgttctgtc
     1261 gggagtcaca cgcaagttcg tgtatgctct caacgccatc aaggaaaaga aagaaaaggc
     1321 tgccgcctga tctttttcaa ttcctgtgtc cgttattatt aaactagtac aaaaacaaaa
     1381 tttctgaagg agaattgtta gccatgacga aagaacaaat catggaagct atcgaaaaga
     1441 tgagcgtcct cgaactgtcc gaactcgtga aagccctcga agacaagttt ggtgtgaccg
     1501 ccgccgcccc catggccatg gccatgccga tggctggtgc cgccgccggc gccgctgccg
     1561 ctgaaaagac cgaatttgac gtcgtgctcg aaaatgccgg cgccaacaag atcaatgtca
     1621 tcaaggtcgt gcgcgagctg accggcctgg gtctgaagga agccaaggat ctcgtcgacg
     1681 gcgctccgaa gaccgtaaaa gaaggcgtgg ccaaggctga cgccgatgcc atgaagaaga
     1741 agctggaaga agccggcgcg aaggtcgccc ttaagtaagc ttcgattcgt tcgaacaaag
     1801 cccgcccggc tctgagcccg gcgggttttt ctttctcgcg tttgatgaag ccgggttgtt
     1861 ggttgtcgaa ctcgtctgtt caggttcatt cgcgggagtg gcggcacgaa attgtgacag
     1921 cagcaggatt ggcgccagca ggaacggcag gaagacatga aaccagattt cgggacggta
     1981 ggacagagtg agcgtcccac ttgtacttgg cggaatctca atgaagatgc cgaaatcagg
     2041 gtctcgaata atcgagagag atctgtctgc ctgatggcat tgccaaccga tcagggggag
     2101 aaccggaaca tagagatggg tgtatcccgt tgtatcagct ggcagagaaa gaaagcccgt
     2161 cgccccatgc cagcgtaaat cgacggccgg gaaagaggca atggcttgtt tgtcccagtt
     2221 tgcatgccag aaaatattga tggaatagga tgcaacgagt gaagctgttt gtgaattatc
     2281 gatatcaaat gaatagatcg cgggtaagca atattcgcgc ggaaacagcc gggccggggt
     2341 ggagacgttc cactgaacct gatcggtcag gactcgcgtg accccgctga gacgcagcaa
     2401 ttggtcgcga tgcacggggt tccgtgtaaa gatgtgtctg atccaacgtt cgcgccccgg
     2461 atgctgaaac ggggggttgt agtcggcgag atggtgaatg ccatgttcac gcccgatcaa
     2521 gggtacgctg gtctgttcga caaagtcata cgcattctgc atgaatcttg ggttcggttg
     2581 tcctggaatg ttgagtacgg catactgcag gccgggagaa accaggactt ttgagccgga
     2641 agcaagacca tgggtgaggg cagtcggacg tcccgcggct ggaatcgaca acggaaccgt
     2701 ctgccgggag ttttcataaa gatcggcgca ctggaagccg atcagcgcgg tcaggatcag
     2761 ataccgatac gagacctggt ggatgcagga caacaggaac aacagtgacg tgatcgcaat
     2821 gccaaacgca taggcctgtt ggctgttttg aaaagagaac atccaaagcg gcgatccaaa
     2881 gccgtcaagc gatctttctg ccaggtactg atgaatcacg tagccgcagc tgccgagcaa
     2941 gagaatgacc agcggagcga tacggggaaa ggaaggctca gcgcgacgca aagaagccag
     3001 accacaggat ccgagagtga gaagacccca gacggtgagc tggatgaagc ttgcgggatg
     3061 gtgcatgtgt cgaaaaacgt cgggaaatac gctggcaaga ttgggcagaa tcatcgacag
     3121 gaggattccg ccggccatca ggcagatgcc cattcggtga atgggtgacc agcgagtgaa
     3181 cgaaatcagg gtcagtatga ggggaagcag gccgaggtag cactggcgcg accacccaaa
     3241 ggctccccat tgctgaatct gcacaacccg gccggtacgg tcgccgaaga tgaagtcgcg
     3301 taacgagcgt ggagagagaa agccgagcag atggcgaggc gcgatggagg tgacttcgat
     3361 ggtgtgcgac gagtcaacgg tgtgcgacat gaactcgacg ctcatcacta catagctgag
     3421 cagaatggaa gcgccgataa gaggaaccag aaacagcacc agtcgcacgg tctttttcca
     3481 ctcggaaggc acgatctgat acccggcaat cagaagtacc ggtgtcatga cccagagata
     3541 ggcatgaccg gagtagatac tgagacacga agcggtcaac cagagttgga tacgaccgcc
     3601 tgcgagcgcc aagaagatcc acgggaccca ggcagtcccg gcggtgatcg gcatccactc
     3661 tagggcatgc agatgaggaa cggctccgat ggccgtgatt ccggcgaaga acgcggcagt
     3721 gcgactgccg gtctgcgagt gcaggaagag ggcgaacccc agaccaagcc agagcaggtg
     3781 tgagaactgg aagagtcgaa agccggtggc cggttcaaag agagcggcga tccaggctgg
     3841 cggataccag gcgcaggttt gtggatcgaa caggaatggc atgccgccgc cccttcccgg
     3901 gtcgacgaat ggaatgaagc cctctgtaag tgcctcgcgt aaaaaggcca ggttcggaag
     3961 accca
//
LOCUS       DAORHD010000314         3948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24955_length_3948_cov_3.673003, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000314 DAORHD010000000
VERSION     DAORHD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24955_length_3948_cov_3.673003"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PKO06_07890"
     CDS             <1..615
                     /locus_tag="PKO06_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69600.1"
                     /translation="VGATTAEETVQTHDPGERLSWRTGPEISERTGDWQEKRMRMQTI
                     CGNCHSPTVMKNHFAQFDKAIALYNHKFAIPAQTILNQLRASGKLDANLFNEPLERIW
                     LFLWHHEGRRARMGVAMMGPDYVQWNGFYEVALGFYFHFIPEAERLQPGITDEIMSRP
                     EHSWYKQTSGTKEEVAVAIRNNVQFWLNTAPEPAAPATGTSRPH"
     gene            complement(656..1090)
                     /locus_tag="PKO06_07895"
     CDS             complement(656..1090)
                     /locus_tag="PKO06_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV69601.1"
                     /translation="MAFIELQEMMARAISFEKQMATFYGSVLSRVTVESSRKTIERLV
                     AEEGKHALKLMEFRPHLPGAGVIQFPPDMPALPSPDDFLASGSDVAALLKAAIKLEQE
                     AEGFYGRLADASPAGSVRELFTALRNFEKGHIDLLTRELAYL"
     gene            1212..3761
                     /locus_tag="PKO06_07900"
     CDS             1212..3761
                     /locus_tag="PKO06_07900"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HNV69602.1"
                     /translation="MPTKTSFPLAKADRARLASEITQADGAEILSVVALAEDGTWEDL
                     RILARGTHDKATAILRGLRPGEILLHNHPSGVLEPSDADLGIAAVCGRSGIGFAIHDN
                     GCKKAYVVVEPFREKKTVRLDPEVCRSFLTSQGRMAKLLAGYEERPGQIGLLESVVKA
                     FNEPCHAVLEGETGIGKSLAYLVPAAHYAHQNKCRVVISTNTINLQYQLANKDIPLLQ
                     RVLPFTIRHCLLKGRGNYLCRQRLADVGDSAEGEILLDADELDQFTRLREWAGRTEDG
                     SLTDLNWEPASSLWEKINADKDACLGLRCPDYDRCFFYRARRAAAEADLLVVNHHLLF
                     ADLELRAATDEFSQTAVIPAYHAVILDEAHNLEDIATAHFGFRTTGFGLQRMLGRLYH
                     RRARREGGLLMSLSSKLALHATEKNVKGIEPVLTEIREKAVPLLQESVDETMRFFDMI
                     GEVTVQNQMREGEFKLRVQPAVRQSDAFRELVRQAERVGDFWQRVQRLVGRVHRTLTD
                     VLESESDGIPQPLQMPLVELGGFPARLARVRTAFELLFPDDAADQEQFVHFFSTTQRR
                     RQRYSSFSSAPLEVAEAMVERCFEKVKTVVLVSATLSTQKNFRFIRGRVGLTEDTYEG
                     PVLEGRFSSPFDYARQAALFVPTDLPEPSWQEFIEATIEPVFRIAQTSGGGTLVLCTS
                     YKHLQTLHERLCARLQQAGLECFRQGDWERSTLLEKFREDGNAVLFATDSFWEGVDVP
                     GSALRNLIITKLPFATPDDPIWAARQEQLEKQGRNPFRELQVPLAAIKLKQGFGRLIR
                     SRGDRGTVWILDKRLVTKFYGDYFLASLPPAPVYSGKHQELCRKAEVFFSR"
BASE COUNT          816 a         1127 c         1188 g          817 t
ORIGIN      
        1 gtcggggcga ccaccgcgga agaaacggtt caaacgcacg acccaggcga gcgcctgtcg
       61 tggcgcacgg ggcctgagat cagcgaacgc accggcgatt ggcaggaaaa gcgaatgcgc
      121 atgcagacca tctgcggcaa ctgccattca ccgaccgtca tgaagaacca tttcgcgcag
      181 ttcgacaagg ctatcgcgct ctataatcac aagtttgcca ttcccgcaca gacgattctg
      241 aaccagctca gagcctcagg caaactggac gcgaatctgt tcaatgaacc gctcgaacgc
      301 atctggttgt ttctctggca ccacgaaggg cgtcgcgcgc gcatgggcgt ggcgatgatg
      361 ggtcctgact acgtgcagtg gaacgggttc tacgaagttg cgctcgggtt ctactttcac
      421 ttcattcctg aagccgaacg tttgcagccc ggaatcacgg acgaaatcat gtctcgaccc
      481 gagcattcct ggtacaagca gacctctggc accaaagagg aagttgccgt tgccatccgg
      541 aacaatgtgc agttctggct gaacacggct cctgaaccag cagcgcccgc aaccggcacc
      601 tcacgtccgc actgatggtc ggaaacaacc ggacggtgac tggtcgagag gtgggttaga
      661 gatacgcgag ttcgcgggtc agcaggtcga tgtgcccttt ttcgaagttg cgcagagccg
      721 tgaataactc acgcaccgaa cccgcgggcg aggcgtcggc gagccgcccg tagaatcctt
      781 ctgcttcctg ttcgagtttg atggcggcct tgagcagggc tgcgacatca gagccgctgg
      841 cgagaaaatc atcaggtgag ggcagcgcgg gcatgtcggg tggaaactga atcactccgg
      901 ctccgggcag atgcggacga aactccatca gcttcagggc gtgcttccct tcctcggcaa
      961 ccagacgctc aatagtcttg cgcgaagact cgacggtgac tcgactcaag acgctgccat
     1021 agaacgtggc catctgtttc tcgaacgaaa tcgcgcgcgc catcatttcc tgaagttcga
     1081 tgaatgccat gtacaattct ccccgaatta gtggtatttg ccccagtata cggcatttca
     1141 tgcagaaagg ggagggggag cattgacttg actggtacac ttgcttaaaa taggcccctt
     1201 cccgaatcat catgccgacg aaaacctcct tccctctcgc aaaagcagat cgggcgcgtc
     1261 tggccagcga aattacgcag gccgacggcg cggagattct ctcggtcgtt gctctggccg
     1321 aagatggcac gtgggaagac ctgcgcattc tcgcgcgcgg tactcacgac aaggcgaccg
     1381 ccattctgcg cgggctgagg ccgggcgaaa ttctgctgca caatcacccc agcggggtgc
     1441 tcgagccttc tgatgccgac ctcggcattg ccgcggtgtg cggtcgctcg gggatcggat
     1501 tcgccattca tgacaacggg tgtaaaaagg cgtatgtggt cgtcgagccg tttcgcgaga
     1561 agaagacggt gcgcctcgac ccggaagtct gtcggtcgtt tctcaccagt caggggcgca
     1621 tggcaaaact gctggccggg tatgaagaac gacccggcca gatcggtctg ctcgagagcg
     1681 tcgtcaaggc cttcaatgaa ccctgccacg cggtgctcga aggggagacc ggcattggca
     1741 agagtctcgc gtatctggtg ccggctgccc actatgcaca ccagaacaag tgtcgcgtgg
     1801 tgatctcgac caacaccatt aacctgcagt accaactggc taataaagat ataccgctgt
     1861 tacagcgtgt tctgccgttc acgatcaggc actgtctgct caagggacgt ggcaattacc
     1921 tgtgccgcca acgcctggca gacgtcggag acagtgctga aggcgagatt ctcctcgacg
     1981 ccgatgaact cgatcagttc acccgattgc gcgagtgggc cggtcgcacc gaagacggtt
     2041 cgttgaccga cctcaactgg gaaccggcga gttcgctgtg ggagaagatc aacgcggaca
     2101 aagacgcctg tctcggattg cgttgcccag actacgaccg ctgtttcttc tatcgcgcgc
     2161 gccgggcggc ggcagaggct gatctgctgg tcgtgaacca tcacctgctg tttgccgatc
     2221 tcgagctgcg cgcggccacc gacgagtttt cgcagactgc cgtgatcccg gcctatcatg
     2281 cggtcattct cgatgaagct cacaatctcg aggatattgc caccgcgcat ttcgggtttc
     2341 gcacgaccgg gtttgggctg cagcgcatgc tcggccgcct gtatcatcgc cgcgctcgtc
     2401 gcgagggtgg tctgctcatg tcacttagtt cgaagctggc gcttcacgca acagaaaaga
     2461 atgtgaaagg tatcgaaccg gtactgacgg agattcgtga aaaggccgta cctctgctgc
     2521 aggaaagcgt tgacgaaacg atgcggttct tcgacatgat cggcgaagtt acggtgcaga
     2581 atcagatgcg tgaaggggag ttcaagctgc gcgttcaacc agctgttcga caatctgatg
     2641 cctttcgcga actggtacgg caggccgaaa gggtggggga cttctggcag cgcgtgcagc
     2701 gcctggtcgg gcgggtgcat cgcacgctga ccgatgtgct cgagtccgag tcagatggta
     2761 ttcctcagcc gcttcaaatg ccgctggtcg agctgggtgg gttccctgcg cgtctggctc
     2821 gggttcgcac ggcgtttgaa cttctgtttc cggatgacgc tgctgatcag gaacagttcg
     2881 tgcatttctt ctcgacgacg caacgtcgcc ggcagcgtta ttcctcgttc tcgtccgcgc
     2941 cgctcgaagt ggccgaagcc atggtcgagc gctgtttcga aaaagtgaaa accgtggtgc
     3001 tcgtatcggc gaccttgtcg acgcagaaga actttcgttt tattcgcggt cgtgtagggc
     3061 tcacggaaga tacgtatgag gggccggttc tggaaggtcg cttctcgtcg ccgtttgact
     3121 atgcgcgtca ggcagcgctg ttcgttccga ccgatctccc cgaaccgagc tggcaggagt
     3181 ttatcgaagc taccatcgag cctgtctttc gcattgctca gacgtcgggg gggggcaccc
     3241 tggtgctttg cacgtcctac aaacatctgc agacgcttca tgagcgcttg tgcgcgcggc
     3301 tgcagcaggc cggactcgaa tgcttccgcc agggagattg ggaacgctcg accttgctcg
     3361 aaaaatttcg cgaagacggc aatgccgtcc tgtttgctac cgacagcttc tgggaaggcg
     3421 tcgacgtgcc cggcagcgca ttgcgcaacc tgatcatcac gaagctgccg ttcgccacgc
     3481 ctgacgaccc catctgggcg gcgcgtcagg aacagctcga aaagcagggt cgcaacccgt
     3541 ttcgtgagtt gcaggtgccg ctggcggcga tcaagctcaa gcaggggttt ggccgtctga
     3601 tcaggtcgcg cggtgatcgc ggcacggtat ggattctcga caagcggctg gtgaccaagt
     3661 tctacggcga ctattttctg gcgtcgctgc cgcccgcccc cgtctattcc ggtaaacatc
     3721 aggagctctg ccgcaaagcc gaagtctttt tctcacgctg aaaagtccta gttcgcgaaa
     3781 cgaacgagag ttcatcagag ccggtgagaa gctgtgagcc acacacttca aaagctctgc
     3841 aagacacgca gtgtgaggga ccgcgagagg cgcaaaaaac tgatcgttgg tgtgcagggt
     3901 catttaattc tcgcccattt tccgttcgca agagcgaaac ttgtgagc
//
LOCUS       DAORHD010000315         3947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_24964_length_3947_cov_3.442960, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000315 DAORHD010000000
VERSION     DAORHD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24964_length_3947_cov_3.442960"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKO06_07905"
     CDS             complement(<1..568)
                     /locus_tag="PKO06_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69603.1"
                     /translation="MSTSSDRPKSFWERHLGAILSGIWGIVAAVLFFSGAFVSFDDYL
                     NALIFQLGYKPDEMREAFWKGEFGTLATESDTPEERARLEKLKALSNPLYLVKKDEVT
                     SSLIQANPGRSEYASIIRFLGSGRQQKIPGVGPRSCDVLDLRLALFDEETKPLITTSL
                     GVSVAPWVGGRGLFKLTEQFYRLPDLFDL"
     gene            complement(587..2179)
                     /locus_tag="PKO06_07910"
     CDS             complement(587..2179)
                     /locus_tag="PKO06_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNV69604.1"
                     /translation="MKLGSICLVLHAHLPFVRHPEHEDMMEERWLFEAITETYIPLLD
                     AFEQLHRAGVDFRITMSLTPPLTTMLSDPLLQERYARHLGQLIELAEKELDRTRHEPE
                     FQRTAQMYYHKFQGVRHLFEHEYQRHIVRGFRRFQDLGKLEVMTCCATHGYLPLMELY
                     RPAVRAQIEVAVRDYERQFGRSPRGIWLSECAYNPGDDAVLADYGLRYFFTDAHGILH
                     AQPRPKYGVFAPIRCPSGVCAFGRDLESSKQVWSAEEGYPGDPDYREFYRDIGFDLDL
                     DYVRPYIHESGIRLNTGIKYHRITGRQSVEKAAYDPQQALDRAATHAGHFLWCREKQV
                     EHLHSLMGSRNPIIVSPYDAELFGHWWYEGPDFLRLLLAKVACDSTTLDLVTPSEYLE
                     RESCIQPASPSASSWGHKGYHEVWLEGSNDWIYRHLHKAAERMIELADRYPQTEGTLR
                     RALNQAARELLLAQSSDWAFIMKTGTTVPYAVKRTKDHCHRFTRLYEEISGNRIDESW
                     LQDLESRDNIFPEIDYRVYCSR"
     gene            complement(2212..3120)
                     /locus_tag="PKO06_07915"
     CDS             complement(2212..3120)
                     /locus_tag="PKO06_07915"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNV69605.1"
                     /translation="MKTYQEKELLKLSKADLYAIARERQIPGRSALSKQELIDAILDR
                     KSTSSPEQPKRPAAGKQTPATTSRTPSPVAERTTATPAAGGRRGLRGSARPAPMEEAF
                     ALSRPPKKPRSSATGMPHEGRLSGHEHECGLPAAYGDNRLVLQTRDPYWAHAYWETGA
                     QRRRELQQQLGLDRYQRSQIILRVYDITGIVFDGANAHRFVDVPVYEETGNWHLHLNR
                     PDGSFIADLGLVSPDGQFHLITRSNAIRLPRGTPSDVIDPDWEVSDENYQRMYKLSGG
                     NTPSGSSFHGAAIPGTSKEPDRGESR"
     gene            complement(3326..>3947)
                     /locus_tag="PKO06_07920"
     CDS             complement(3326..>3947)
                     /locus_tag="PKO06_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69606.1"
                     /translation="APPAEEEKKEGEEAPAAEAASSESSAPEAAAAGAEEKKPEEEAS
                     AESEAPAGEAEEKVAFFTNKRTSYPEFQRPANALREVAEPATRVLFTSDFEFSDPGAM
                     SKDFPEAAFTFKAANEQPNVPRGRFELFTVILGKGENQKEFFFKAEDGIPDQKLSWDD
                     GTCIYQVAFEEVNATTAYIQDVYGEGSRPSPLFTRIKTSIVIQRKQ"
BASE COUNT          748 a         1122 c         1226 g          851 t
ORIGIN      
        1 gcaggtcgaa gaggtctggc agtcgataaa actgctcggt gagtttgaac aagcctcttc
       61 cgccgaccca gggagcgacg gagactccga gagaggtggt gatcaggggc ttggtctctt
      121 cgtcgaacag cgcgagtcga agatcgagga cgtcgcagga gcggggcccg acgccgggaa
      181 tcttctgctg tcgacccgac ccgagaaagc gaatgatcga tgcatactcg ctgcgccccg
      241 ggttcgcttg aatcagcgat gaggtgactt catccttctt gaccagatac agggggttcg
      301 ataaggcctt cagcttttcg agacgggctc gctcttcagg agtatcggac tcggtcgcca
      361 gtgtaccgaa ctcgcccttc cagaacgctt cacgcatctc atcgggtttg tagcccagct
      421 gaaagatcag ggcgttcagg taatcgtcaa aactgacaaa cgcgcccgaa aagaagagaa
      481 cggcggcgac gataccccag attcctgaaa gaattgcgcc aagatgtcgt tcccagaatg
      541 attttggtct atctgaactc gtactcataa aagttcctct cttgcatcag cggctgcaat
      601 acacccgata atcgatctcg ggaaagatgt tgtcgcggct ttcgaggtct tgcagccatg
      661 actcatcgat acgattcccg ctgatttctt catacagccg ggtgaagcgg tgacaatgat
      721 ctttggttcg cttgaccgca tacggcacgg tggtgccggt tttcatgatg aaagcccagt
      781 cagacgactg cgcgagcagc agttctcgtg ctgcctgatt cagcgcgcga cgaagcgtgc
      841 cctcggtctg agggtaccgg tcggccagtt cgatcatgcg ctcggcggcc ttgtgcaggt
      901 ggcggtagat ccagtcgttg ctgccctcga gccagacctc gtgatagccc ttgtgccccc
      961 aggaggaggc agagggagat gccggctgga tgcacgattc gcgctcgaga tactcggacg
     1021 gagtgaccag gtcgagtgtc gtgctgtcac aggccacctt ggccagaagc aagcgcagaa
     1081 agtccgggcc ctcataccac cagtggccga acaattccgc gtcatacggc gagacgatga
     1141 tagggtttct gctgcccatc agagaatgca gatgttcaac ctgcttctcg cggcaccaca
     1201 gaaagtgccc ggcatgcgtg gctgcgcgat caagcgcctg ctgcgggtca taggcggcct
     1261 tctcgacgct ctggcgcccg gtgatgcggt ggtacttgat gccggtattg aggcgtatac
     1321 cgctctcgtg gatgtagggg cgaacatagt cgagatcgag atcgaagccg atgtcgcgat
     1381 agaactcccg gtaatcgggg tcaccaggat acccctcttc agccgaccag acctgcttgc
     1441 tgctttcgag atcgcgtccg aatgcgcaca ccccggaagg gcagcgaatc ggagcgaaga
     1501 ctccgtattt gggccgtggt tgcgcgtgca gaataccgtg cgcgtcggtg aagaaataac
     1561 gcaacccgta gtcggccaga acggcgtcat cgcccgggtt gtaggcgcac tccgacaacc
     1621 agatgccgcg tggcgagcgt ccgaactgtc gttcgtagtc acgcacggcc acttcgatct
     1681 gcgcacgcac cgccgggcgg tacagttcca tcaggggcag atacccgtgc gtggcacagc
     1741 aggtcatgac ctcgagcttg ccgagatcct gaaaccggcg aaagccgcgc acaatgtgac
     1801 gctgatactc atgctcaaaa aggtgccgca cgccctggaa cttgtggtaa tacatctggg
     1861 ctgtgcgctg gaactcaggc tcgtgacgtg ttcgatcgag ttctttctcg gccagctcga
     1921 tcaattgtcc gaggtgcctg gcgtagcgtt cctgaagtag agggtcgctc agcatggtcg
     1981 ttaggggcgg tgtcaacgac atggtaatgc ggaaatcgac gccggcccga tgcagctgct
     2041 cgaaggcgtc gagcaatgga atataggttt cggtgatggc ttcgaataac caccgctctt
     2101 ccatcatgtc ttcgtgttcg gggtgacgga cgaaagggag atgggcgtgc agaacgaggc
     2161 agatggaacc gagcttcata cggtggaccc ctggtggtgc atcagcgtga ctcagcgcga
     2221 ctcgccgcgg tcgggctctt tcgatgttcc cggaattgcg gcgccatgaa acgagctgcc
     2281 ggagggtgtg ttgcctccgg atagtttgta catacgttga tagttctcgt ccgagacttc
     2341 ccagtccggg tcgatgacgt ctgatggtgt gccgcgcggc aggcgtatcg cgttgctgcg
     2401 ggtgatcaga tgaaactgtc cgtccgggct gaccaggccg agatcggcga tgaatgagcc
     2461 gtcggggcgg ttgagatgca gatgccagtt gccggtttct tcatacacgg gaacatcgac
     2521 gaagcggtgg gcgttcgcgc cgtcaaagac gatgccggtg atgtcgtaga cgcggagtat
     2581 gatctgtgac cgttgataac gatcgagacc cagttgctgc tgcaactcac gccgccgctg
     2641 tgcgcccgtc tcccagtagg catgggccca gtatgggtcg cgcgtctgca agaccagccg
     2701 gttgtcgcca tacgccgccg gcaacccgca ttcgtgttcg tgtcccgaaa ggcgaccctc
     2761 gtggggcatt ccggtcgccg aagaccgcgg ctttttcggt ggccgcgaca gcgcaaacgc
     2821 ctcttccatg ggagccggtc gggctgagcc gcgcaggcct cgacgcccgc ccgctgccgg
     2881 cgtcgcggtc gttcgttcgg cgaccggtga aggggtgcgt gacgtggttg ccggtgtttg
     2941 cttccctgct gccggtcgtt tcggttgctc tggtgaactt gtacttttgc ggtcaagaat
     3001 ggcgtcgatc agttcctgct tcgacagcgc cgaccggccg ggaatctgtc gttcgcgggc
     3061 aatcgcgtac aagtcggctt tgctcagttt cagcagttct ttttcctgat aggtcttcat
     3121 ggacatgctc cttgcgagaa tctctcgctt gaaggttcgc ctggcgtgcc ggtgatctca
     3181 cgcatcacgg tcaggagtcg tctcccagat accggtcgat gagtgcgggc agcagaacga
     3241 ggagaaagta gtagtgcagg cccatgaaca gcccgaacag gcaaccgaga atctgataga
     3301 tcatggggcg gtttctcctt gtgacctact gcttgcgctg aatgacgata ctggtcttga
     3361 ttctcgtaaa cagcggcgaa ggccgcgagc cctctccata gacgtcctga atgtaggcgg
     3421 ttgtggcgtt cacttcttcg aaagccacct gatagatgca ggtgccatcg tcccagctca
     3481 gcttctggtc cggaatgccg tcttcggcct tgaagaaaaa ctctttctga ttttcccctt
     3541 ttccgagaat gacagtgaac aactcgaaac gcccgcgggg gacattgggc tgttcgttgg
     3601 ccgccttgaa ggtgaaagct gcttcgggaa agtccttcga catggccccg gggtccgaga
     3661 actcgaagtc ggaagtgaac agcacgcggg ttgcgggttc ggcgacttcg cgcagggcat
     3721 tggccggccg ctgaaactcc ggatacgagg tacgtttgtt ggtgaagaag gcgacctttt
     3781 cttcggcctc gcccgccggc gcttcgctct ccgcgctcgc ctcttcttcg ggcttcttct
     3841 cttccgcgcc agccgctgcc gcctcgggcg ccgacgattc cgacgacgcc gcttcggctg
     3901 ccggcgcttc ctcgccctct tttttctctt cttccgccgg aggcgcc
//
LOCUS       DAORHD010000316         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25061_length_3937_cov_3.148892, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000316 DAORHD010000000
VERSION     DAORHD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25061_length_3937_cov_3.148892"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PKO06_07925"
     CDS             complement(<1..1012)
                     /locus_tag="PKO06_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69607.1"
                     /translation="MNPTPEALNLNKKKDQSDTSVNSDSADSLSDKIKGKLAGLIPGM
                     PEINVLPPTSYGISLLGVAMHLHPLMDPWAKWEWPFMGMGWRLFRIPNTGVGQNVTHL
                     FGSGGIFPPMTRSIEGFVLKRYRQWNLGIVGWPMGPYPLVGNQITIPFTPPILLPPIP
                     IPLWHTHDIVNKYDYNLWLLKAPKDKDGSEGDEDVVTYDPGIQENSPPNLYSIEQYAK
                     KATYYYTSQADFYKDLPNRLIKVGDSQALRLNGITFIADSLVIPPVGSGTLKVTGKGA
                     IVVGGNIILTGNIEDAYTPEQERSKETPRTIFSLIARQGGLIFDSNASANHAKIEGCV
                     YTD"
     assembly_gap    1089..1098
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1099..2542)
                     /locus_tag="PKO06_07930"
     CDS             complement(<1099..2542)
                     /locus_tag="PKO06_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69608.1"
                     /translation="MSNRGNVFIVIVAVLGIIFTMATFFMQATIEEKGQTEYSHRAVQ
                     TQCLAEAAMERAVRLLNQEMNDPEKAKDKTTIAGKLRQPMSVAAGSTPGTSGLGSSLG
                     QDEPLVVPDDMAELIVMKKEDLQLETHELDELVTFMVGDDQKVKYEVEVKVGLSSAYR
                     VAPGPSVEDAAHRYYVPAVDVPWSINPLVKGFLENKGFVALRMKFPDDWKWLQFCIPI
                     KIGPLTIYEVDLVDLLNKAFPATSEYTQYFEVHKLLATLFPSFYPYDIVFAKDIFPSL
                     ADKLGPGVTPPPLDPKKPMEKYGFMSLESKATITFPDSRSITRNISASKEFKCADVQP
                     IAPLHSLFVSNLKDEALNLNDIGGNVYVNNFAGFGAVKNGPGADNKEKYEFPGLVRFN
                     GNKPMVCNVSFIGNPMGPSLGEGGNDGVLAKLGRGAEVPIMLDLGCEMDGIDTLGKDA
                     NLVAFVPKKYQDKYSAEINSSVTWAEHSTNP"
     gene            complement(2557..3363)
                     /locus_tag="PKO06_07935"
     CDS             complement(2557..3363)
                     /locus_tag="PKO06_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69609.1"
                     /translation="MDDLREKLSKIDFSDIRVLLVGLVLLCIPLLFLVYIMRAPGGGG
                     FGMSGAREKLEVTGRKGFSFARREAPAQKTGWTGGSAGSPFVPSAAESEWNSAVARIE
                     QESYNAPDVPGMTPEGKMLLEAELNPKMRRAQMFMNANRPKEALPILEEILDNPNSNP
                     VLQLSASSLLCEMYEKEGKTAELAAEFKRLMSLMSKVPKIGGFAANLEYAMKEMSKIP
                     LMLQKWKSDPRGSQQIQDFLTKNNMKVTPDEYLNQINQTFKDMVPGYPNL"
     gene            3619..3909
                     /locus_tag="PKO06_07940"
     CDS             3619..3909
                     /locus_tag="PKO06_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNV69610.1"
                     /translation="MSVKPTGNRVLLEVKTHEAKSKGGVILPDTASKDRPHEGKVIAI
                     GPGKVDKHGKHVPIDVKVGNTVYFSEYAGTEVEIEGKKHLLVKDDELLAVSE"
BASE COUNT          840 a         1065 c         1082 g          940 t
ORIGIN      
        1 tgtcggtata gacgcagcct tcgatcttgg catggttggc tgatgcgttc gaatcgaaga
       61 tcagaccacc ctgtcgggcg atcaacgaga agatcgtgcg cggcgtttcc ttcgaccgct
      121 cctgttcagg ggtgtacgcg tcttcgatgt tcccggtcag aatgatattg ccgccgacaa
      181 caattgcgcc tttcccggtt actttcagcg tgcctgaacc gacgggcgga atgaccagag
      241 aatcggcgat gaacgtgatg ccgttcagcc gcagagcctg cgaatcgccc accttgatca
      301 ggcgattggg aagatctttg tagaagtccg cctgtgacgt gtaatagtac gtcgccttct
      361 tcgcatactg ctcgatcgaa tacagattcg gcggcgagtt ttcctgaatg cccgggtcgt
      421 aggtgaccac gtcttcatca ccttctgaac cgtctttatc tttcggagct ttcagcagcc
      481 agaggttgta gtcatacttg ttcacgatgt catgcgtatg ccagagcgga atcggaatcg
      541 gcggcagcag aatcggcggt gtgaacggaa tcgtgatctg gttgccgacc agcggatacg
      601 gtcccatcgg ccaccctacg atcccgaggt tccactggcg ataacgcttg agcacgaatc
      661 cttcgatcga ccgcgtcatc ggcggaaaga tgccgccgga gccgaacaga tgggtcacat
      721 tctgaccgac gccggtattc ggaatgcgaa acaagcgcca ccccatcccc atgaagggcc
      781 attcccactt ggcccacgga tccatgagcg ggtgcagatg catcgccacg ccgagcaagc
      841 tgataccgta ggaagtgggc gggagaacgt tgatctcggg cataccggga atcaatccgg
      901 ccagtttgcc cttgatcttg tccgacagac tgtccgcact gtcactgttg acggatgtgt
      961 cgctctgatc cttctttttg ttgaggttga gggcttcggg tgtcggattc aaggtgctcg
     1021 cgtcacccgt tgaatcgttg ctgcccgcac ctccagccaa ggttccggcg gagccacctt
     1081 ccttcgggnn nnnnnnnntc gggttggtgg aatgctcggc ccaggtgacg ctcgaattga
     1141 tctctgcgga gtatttgtcc tggtactttt tcggcacgaa tgccacgaga ttggcgtctt
     1201 tgccgagagt atcgatgcca tccatttcgc agccgaggtc gagcatgatc ggcacttcag
     1261 cgcctcgacc aagtttcgcc agcaccccgt cgttgccgcc ctcgccaagg cttggcccca
     1321 tggggttgcc gatgaacgag acattgcaca ccatgggctt gttaccgtta aagcgcacca
     1381 ggcccgggaa ctcatatttt tccttgttgt ccgcgcccgg tccgtttttc accgcgccga
     1441 acccggcaaa gttgttcacg taaacattcc cgccgatgtc gttcaggttc agtgcctcgt
     1501 ccttgagatt cgaaacgaac aaactgtgca gcggcgcgat cggttgcacg tcagcacact
     1561 tgaattcctt gctggcggaa atgttgcggg tgatcgatcg cgagtcggga aacgtaatcg
     1621 tcgccttgga ttccagagac atgaacccgt acttttccat gggtttcttc ggatcaagcg
     1681 gcggcggagt cacgcctggt ccgagtttgt cggccagact ggggaatatg tctttggcga
     1741 agacaatgtc atacggataa aaacttggaa acaacgtcgc cagcagctta tggacctcga
     1801 aatactgcgt gtactcagag gtggccggga atgccttgtt cagcaggtcg accagatcga
     1861 cttcgtagat ggtcaaaggg ccgatcttga tgggaatgca gaactgcaac cacttccaat
     1921 cgtccgggaa tttcatgcgc agggcgacga agcccttgtt ttcgagaaag cccttcacga
     1981 gcgggttgat actccagggc acatcgacgg cggggacata atacctgtgc gcggcgtctt
     2041 cgaccgacgg gccgggagct acgcggtagg cgctgctgag gccgactttc acctcgactt
     2101 cgtatttcac tttctggtcg tcgccgacca tgaacgtgac cagctcgtcg agttcgtgcg
     2161 tctcgagttg caggtcttct tttttcatga cgatcaactc ggccatgtcg tcgggaacga
     2221 cgagaggctc atcttgcccg agcgatgaac caaggccaga ggtgcctgga gtcgaaccgg
     2281 ccgcgaccga catgggctgg cgcagttttc cggcaatcgt ggtcttgtct ttcgccttct
     2341 cggggtcatt catttcctga tttaacaggc gcacggcccg ctccatggcg gcctctgcga
     2401 gacattgagt ttgtaccgcg cgatgtgaat actcggtctg gcccttctct tcgatcgttg
     2461 cctgcatgaa gaaggtggcc atcgtgaaga taatgccgag aaccgcgacg atgacgataa
     2521 agacgtttcc tcgattactc atggcgcact tcctccttac aggttcggat acccggggac
     2581 catgtccttg aaggtttggt tgatctgatt gagatactcg tcaggagtga ctttcatgtt
     2641 gtttttcgtg agaaaatcct gaatctgctg gctgcctcgc ggatcagact tccacttctg
     2701 cagcatgagc ggaatcttgc tcatttcttt catcgcgtac tccaggttgg ccgcaaaccc
     2761 gccgatcttg ggaaccttgg acatcaggct catcagtcgc ttgaactcgg cggcaagttc
     2821 ggcggttttt ccttcttttt cgtacatttc acagagcagc gatgaggcgg acaactggag
     2881 taccggattg gaattgggat tgtcgaggat ctcttcgaga atcggcagag cttctttcgg
     2941 acggttggca ttcatgaaca tctgagcccg cctcattttc gggttgagct ctgcctcgag
     3001 gagcattttt ccttcggggg tcatgcccgg cacatcaggc gcattgtatg attcctgttc
     3061 gatacgggcc acggcgctgt tccattcaga ttcggccgcc gagggcacaa atgggctgcc
     3121 cgcggaaccg ccagtccagc cggttttctg agcaggggcc tcgcgacggg cgaacgaaaa
     3181 cccttttcgg cccgtcactt cgagcttctc gcgtgctccc gacatgccaa accctcctcc
     3241 acccggggct ctcatgatgt acaccagaaa cagtaacgga atacacagca aaacgagacc
     3301 caccagtaat acgcggatat cgctgaaatc gattttggaa agtttttcgc gcaggtcgtc
     3361 cattgtttct ccctctcccc attctacaca ggtacatgaa ctatttggga acaaatattg
     3421 ttgctggtca taatttctcg ttttgcagag ataatgtttg tatagattgc aaatctttcg
     3481 acaaacgtct gccacgcgcg attcacatct ttactctcct gaagcggatg ttttcgaaga
     3541 accgcgaaat cggtaaaatt gcgacctgtt agcactcaat cgcaatgagt gctaacaacc
     3601 gaaaaaacgg aggattatat gagcgttaag ccaacaggga atcgagtgct tcttgaagtc
     3661 aagacgcacg aagcgaagtc caagggtggt gtcatactgc ccgacacggc cagcaaagat
     3721 cgaccgcatg aaggcaaagt gattgctata ggcccgggca aagtcgacaa gcacggaaag
     3781 catgttccga tcgacgtgaa ggttggcaac accgtgtact tttccgaata cgccggcacc
     3841 gaagtcgaga tcgaggggaa gaaacacctt ctggtcaaag acgatgagct gcttgccgtc
     3901 agtgaatagt tagcaaattc aattccacaa aggagtt
//
LOCUS       DAORHD010000317         3933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25105_length_3933_cov_3.298092, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000317 DAORHD010000000
VERSION     DAORHD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25105_length_3933_cov_3.298092"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="PKO06_07945"
     CDS             complement(<1..1294)
                     /locus_tag="PKO06_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69611.1"
                     /translation="MKPSFADGLYAVFDVGSFAAKAAIIERTAGKVRLASIEEEPLKS
                     LTDFPSEVEFRDALIQSLQAIAGRLPLGQCREVIGLFSHRELQVKLVDLPPQITPEQF
                     DSVLGFEAKKLLSPNYRSEAFVYSYRVLRESPPFVALAVIPQSHLQKTIELYHGAGIK
                     LSGLYPEIFGELAAKETMPASGSPVYTMINVGHASTHMTIFSGGEPRFTRQIPSGVSE
                     IPERVTGNEPEIYTQKIRFSFDYCRAVFKLGQIDEIGFFGGGALKPGFLDFTREYFHP
                     SKVSVIDLSSCLDISPALAQRGGDATRLAPFLPGITTYLAHSRALATDRSNMYMQWER
                     KESDERWKNLSHLLPAGVALAGLLVGVVLLAIWGMVLQTELDKANQEAKLSEIEVTRA
                     RTALVRMESQRKPGASLAPFEQKLLEPLLSDHFAGDAIL"
     gene            complement(1291..1725)
                     /locus_tag="PKO06_07950"
     CDS             complement(1291..1725)
                     /locus_tag="PKO06_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69612.1"
                     /translation="MNLPHVSPPCSRHGFALFLAMMVTTLLLFLVFGARSVSRASLDL
                     GRSAALESVAFQAADGGLERGLGRLHRSFAPCTFSYLSPLTPVRTVQVTVTAAGDRNR
                     LDLSASATVLDAGRPVAVRCLSRKGITQSGGKVGVGMFLEEP"
     gene            complement(1722..2126)
                     /locus_tag="PKO06_07955"
     CDS             complement(1722..2126)
                     /locus_tag="PKO06_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69613.1"
                     /translation="MKRGFTLMEVVVAVLVMGLVSTGLLNLLHWGRLRYHAIDDGWRE
                     REVLKVLHRTLRREISAGNLDFVSTVPKQFARLFPTTLPLHCTSIDMRPYASDTVFVR
                     PNLYIDRNRNGREDPLESLAAQVWCFRIRSQP"
     gene            complement(2357..2530)
                     /locus_tag="PKO06_07960"
     CDS             complement(2357..2530)
                     /locus_tag="PKO06_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69614.1"
                     /translation="MNQRRGLSLVEILLALIIMAGSVVSILRGLEVQESLEHQARFET
                     QAALYAERELELL"
     gene            complement(2527..2937)
                     /locus_tag="PKO06_07965"
     CDS             complement(2527..2937)
                     /locus_tag="PKO06_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69615.1"
                     /translation="MLSRCGFSLVELLIVVMLLGVLATTGIRFFSRAANDTRLRTGND
                     AIESMLHACQQRARQRGFPVQLVWSGNAFQISGSPAANFPVPELTPATRTRLSDLQFF
                     ATGTLLLGQRVSSITVSLIVPGAEPATVFVPLLP"
     gene            complement(3053..3568)
                     /locus_tag="PKO06_07970"
     CDS             complement(3053..3568)
                     /locus_tag="PKO06_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69616.1"
                     /translation="MLRKGFTLVELLIVVLVIGILGAIAIPRYQSIAATARAKSCLSN
                     QKAIESLIALWEAKNFELDAGKNRFSWIDRQGNMRWRYSNAVLNLNELAKDPKLFICP
                     EASHRWGYTWPGASYRFYNTDNTGLWILGSTGSMGGRGVVCALRLGPRWIGMNGQMGP
                     DGSKDSAHHYW"
     gene            complement(3617..3895)
                     /locus_tag="PKO06_07975"
     CDS             complement(3617..3895)
                     /locus_tag="PKO06_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV69617.1"
                     /translation="MARGEAVSVGMSRRKDLFPDSLILMVDVGERSGNLGDMLSKAAT
                     IYERDLETTLEAIVSLVEPLLVIFLAFFVVGVALAMYLPLFDIFKAIK"
BASE COUNT          873 a         1115 c         1198 g          747 t
ORIGIN      
        1 acagaatcgc atcaccggcg aaatgatcag aaagcagcgg ctcaagcagc ttttgttcga
       61 acggtgcgag actcgccccg ggcttgcgtt gactttccat tcgaaccagc gccgtgcgag
      121 cgcgtgtgac ctcgatttca ctgagcttgg cttcctgatt ggccttgtcc agttccgttt
      181 gcaggaccat accccagatg gcgagaagga cgacacccac gagaagtccg gccagagcga
      241 ctccggccgg aagcaggtgg gacagattct tccagcgctc gtctgattcc ttgcgctccc
      301 actgcatgta catgttcgat cgatcggtgg ccagggcccg tgagtgagcc agatacgtcg
      361 tgattccggg cagaaagggg gccagacgtg tagcgtcacc gccgcgttga gccagagccg
      421 ggctgatgtc gagacaggag gacaggtcga tcaccgacac tttgctgggg tggaagtatt
      481 cgcgggtaaa gtcgaggaat cccggtttca gtgccccgcc tccgaagaaa ccgatttcgt
      541 cgatctgacc gagtttgaaa acggcgcggc agtagtcaaa gctgaagcga atcttctggg
      601 tgtagatctc gggctcgttc ccggtgaccc gctcgggaat ctcggatact cctgagggaa
      661 tctgcctggt gaagcgcggc tcgcctccag agaagatggt catatgggtg ctggcatgtc
      721 cgacgttgat catcgtatac accggagaac cggaggcggg catggtttcc ttcgcggcga
      781 gttcgccgaa aatctcggga tacagacccg acaatttgat gccagcccca tgatacagct
      841 caatggtctt ctgcagatgc gactgcggaa tgacggcgag cgcgacaaat ggcgggcttt
      901 cgcgcaagac gcgatacgaa tacacgaacg cttcgctgcg atagttcgga gacagcagtt
      961 tcttggcctc aaagccgagc acgctgtcga actgttccgg agtaatctgg ggtggcaggt
     1021 cgaccagctt cacctgcagc tcgcgatgag aaaacaaccc gatgacttcg cgacattgtc
     1081 cgagagggag tcggccggca atagcctgca gtgactgtat aagggcatcg cgaaactcga
     1141 cttcagaggg aaaatccgtg agtgatttga ggggttcttc ctcgatcgat gccagtcgca
     1201 cttttccggc ggtgcgctcg atgatcgcgg ccttggcggc gaaactgccg acatcgaata
     1261 ccgcgtagag accgtctgca aacgagggtt tcatggttcc tccaggaaca tgccgactcc
     1321 gaccttccca ccggattgag tgattccctt ccggctcagg caacgtaccg cgactgggcg
     1381 tcccgcgtca agaactgttg cggatgctga caaatcaaga cgattcctgt ccccggccgc
     1441 tgtcacggtg acctgtacgg tgcggacggg cgtcaagggc gacagatagg aaaatgtgca
     1501 gggggcgaac gaacgatgca ggcgtcccag acctcgctcg aggcccccgt cggcggcctg
     1561 aaacgccacg ctctcgagag ctgccgaccg cccaagatca aggctggcgc gagagacgga
     1621 acgagcaccg aaaacgagaa acagcaacag ggtcgtcacc atcatggcca gaaacaacgc
     1681 aaaaccgtga cgggagcacg gtggtgaaac gtgaggcagg ttcatggttg actccggatg
     1741 cgaaagcacc agacctgcgc cgcgagagat tccaggggat cttctcgtcc gttgcggttg
     1801 cgatcgatgt agagattcgg tctgacaaaa accgtatcag aagcatacgg tcgcatgtcg
     1861 atactcgtac aatgcagcgg cagagttgtc ggaaacagtc gtgcgaactg tttcggcacg
     1921 gtcgagacaa aatcgaggtt tccggcggag atctcgcgcc gcagggtgcg atgcaggact
     1981 ttgagtacct cgcgctcgcg ccagccgtcg tcgatggcat gataccgcag tcgcccccag
     2041 tgcagcaggt tcagcagacc ggtgctgacc agccccatca ccagcacggc gactaccacc
     2101 tccatgaggg taaagcctcg tttcatgtgc gcctgttccc tcccgaacgt ggcgctatcg
     2161 ccggctccag gggcagtgcc acgaatgact cacacgcgaa gagattttcg ctcttcgtga
     2221 ttcgaacaat gagtcgacag acgttatcct gatcgggcgc ggtccagaca agacgtgttt
     2281 tccagccgcc gggcagacga aatcttcccg gtgcccctcg cggacccagg gggcgtcttc
     2341 ccccgagcag atcgctttac aggagttcga gttcgcgctc ggcgtagaga gcggcctgag
     2401 tttcaaagcg ggcctggtgt tcgaggctct cctgaacctc gagcccgcgc agaatcgaga
     2461 ccacgctgcc ggccatgatg atgagcgcca gcagaatctc aaccaaagac aatccgcggc
     2521 gctgattcat ggcagtaagg gtacgaagac ggttgccggc tcggcacccg ggacgatcag
     2581 agacaccgtg atggatgaaa cccgctgtcc cagtagcaac gtgccggtgg caaagaactg
     2641 cagatcggac aaacgggtgc gggtggccgg ggtcagttcg ggaaccggga agttcgctgc
     2701 cggggagcca ctgatctgga aggcgttccc tgaccagacg agttgtactg ggaacccgcg
     2761 ttgtcgcgcc cgctgctggc aggcatgcag catcgattcg atcgcgtcgt tgccggtacg
     2821 cagtcgcgta tcgttggcag ctctggaaaa aaagcgaata ccggtggtgg ccagaactcc
     2881 gagaagcatg accacgatca gcagttctac cagcgaaaag ccgcatcgag aaagcatgaa
     2941 tacactctga tcctgggact tccggcgggg tcttcgatag tcgattttcc cctgccagtg
     3001 gccaggaacc gttcgtaagg cgggagtgcc ggtgaaaatc ggcacccatc cgttaccagt
     3061 aatggtgcgc gctgtccttc gagccgtccg ggcccatctg cccgttcatg ccgatccagc
     3121 ggggcccaag acgcagagcg cagacgacgc cacgaccgcc catggagccg gtggaaccga
     3181 ggatccacaa gccggtgtta tcggtattgt agaaacgata actggcgccc ggccaagtgt
     3241 acccccagcg gtgtgatgcc tcaggacaaa tgaaaagttt cgggtctttg gcgagttcgt
     3301 tgaggttcaa cacggcgttg gaatagcgcc agcgcatgtt gccctgacgg tcgatccagg
     3361 agaaacgatt cttgcccgcg tcgagctcga aattcttggc ttcccagagc gcgatcaggc
     3421 tttcgatggc tttctggttg gaaagacagg acttggcacg agccgtggcc gcaatgctct
     3481 gatatcgggg aatggcgatg gcgccaagaa taccgatgac cagcactacg atgagcagtt
     3541 caaccagcgt aaagcctttt ctcaacatgt gttcctccag gacggaatca aagggtgacc
     3601 cgtgacaatc aacactctac ttgatggcct tgaagatgtc aaacaagggc agatacattg
     3661 ccaatgccac gccgacgaca aagaatgcca ggaagatgac cagcaggggt tcgacgagcg
     3721 aaacgatggc ttcgagcgtg gtttcgaggt cacgttcgta gatcgtcgcg gctttgctta
     3781 acatgtcgcc aagattgccg cttcgttccc cgacgtcgac catgagaatc aagctgtccg
     3841 ggaacagatc tttgcgccga ctcatgccaa ccgatacggc ctcgccgcgc gccacgccac
     3901 ttcgctcagc gaggactggt cactggtggt gcg
//
LOCUS       DAORHD010000318         3932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25118_length_3932_cov_3.484395, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000318 DAORHD010000000
VERSION     DAORHD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25118_length_3932_cov_3.484395"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1216
                     /locus_tag="PKO06_07980"
     CDS             104..1216
                     /locus_tag="PKO06_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69618.1"
                     /translation="MNTREQELIAAIARKDEVTARSLMTGDLEPHTAEAAAKAAMEAG
                     MIELFQELIRRALPLVDSLGETWLMKAAFMGNLLFVQLLHGGLGMPVNHQSRSGATAL
                     IRGCLSGKTDVVRYLVDHGANLNAEEKIGGGSSVLMGWESFCGTPLMAAIVAGNDEAV
                     RLLIERGADLTIRNSRTFDALEMAIRLHRLDAMNALAKARGLTLIDLLMTAARLNREV
                     VRHLVESAHADVNARDAHGMTAVHQAARYGNYSFIHERVSRGAVNGAVEMIEYLLSVG
                     ARVDDPGPGGATPLMYAAETANGGLIDFLLGKGANPDARDAQGRCAAEYVKYPEAPPE
                     SWENAYEAWGWECHGLSDLENRLIKSMRQPPTPPKK"
     gene            complement(1408..1659)
                     /locus_tag="PKO06_07985"
     CDS             complement(1408..1659)
                     /locus_tag="PKO06_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69619.1"
                     /translation="MPTDLAQSAGGEDVLRGIAQKIRDGFFQWIVSGLKVEREQDRKP
                     LASLAFATIEGFVLLDALDSPHLIDSALKGLSRRRASRI"
     gene            complement(1851..2153)
                     /locus_tag="PKO06_07990"
     CDS             complement(1851..2153)
                     /locus_tag="PKO06_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNV69620.1"
                     /translation="MKTKKLPPIHPGMILLKEFLEPLGLSQYRLAQDIGVPRRRINEI
                     IHGIRGVSADTSLRLARFFGLSERFWLNLQTRFDVEVEKDRLADRLYQEVKVLKVA"
     gene            complement(2163..2444)
                     /locus_tag="PKO06_07995"
     CDS             complement(2163..2444)
                     /locus_tag="PKO06_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNV69621.1"
                     /translation="MIKSFRCKETERLFHREVARRLPSEIIESARMKLEMLDAAEVIE
                     DLRIPPSNHLEKLSGNRAGQYSIRINRQWRVCFRWQSGNAFDVDVADYH"
     gene            complement(2553..>3932)
                     /locus_tag="PKO06_08000"
     CDS             complement(2553..>3932)
                     /locus_tag="PKO06_08000"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HNV69622.1"
                     /translation="AVLARHHMFRVLLRVMTGVAIPRGEAEFWGSRFPRLAAADYLAS
                     LFLRPTESLTVFLQQFGRGLRLSRDKDCLTVLDFIGQAHHRYRLGLRYRALTMASDRP
                     LAEQIRDGFAHLPAGCTITLERQARHYVLDNLKRAVDHSRPKLVQAIREFENESGRAP
                     SLAAFLEFHHLEPDAIFSRGAWSRLCVAAGIREDFADPDEARLTKGLQRLQHIDDPAY
                     ITSILALVDLPPGHSDELSQGQKAHLLMLHFVLWDANDRPGSFLESLSKLQGNKHLCN
                     DLRELLAYRLAKVATIAPPLDVPFPCPLRLYSSYIREEILSGLGYWTLARQPPVRQGV
                     VHIQTIRADAFFVTLNKHESDYSPTTMYEDYAISDRLFHWQSQSTTSDSSPTGQRYIN
                     HRSHGGTILLFVRENRKLPSGLAAPFHFLGPADYEGHKGSRPISFTWRLRHPIPAHLR
                     NETSRLLTG"
BASE COUNT          883 a         1090 c         1193 g          766 t
ORIGIN      
        1 cgcgcccgtg agtgatcgac tcaccggagt tggcactgca catggcggcc ggccccacgg
       61 gcccggcgag aaacatcttt gcgcgtgata gtatgagaca ccgatgaata ctcgcgaaca
      121 agaactgata gcggccattg cccggaagga cgaagtgacg gcgcgaagcc tgatgacggg
      181 cgatctggag ccgcacacag ccgaggcggc ggcaaaagcg gccatggagg cggggatgat
      241 tgagttgttt caggaactga ttcggcgggc gctcccgctg gttgattcgt tgggcgaaac
      301 ctggctgatg aaagcggcat tcatgggcaa tctgctcttc gttcagctct tgcacggtgg
      361 cctcggcatg ccggtcaatc atcagagccg atcaggggcc accgccttga ttcgcggctg
      421 tctgagcgga aagaccgatg tcgtgcgcta tctggtcgat cacggcgcga acctcaacgc
      481 cgaggaaaag atcggcggcg ggtcctcggt gctcatgggc tgggaaagct tctgcggcac
      541 accgctgatg gcagccatcg tggccggcaa cgacgaagcg gtgcgtctgc tcatcgaacg
      601 cggagccgac ctgacgattc gcaattcccg cacttttgac gccctggaaa tggccatcag
      661 acttcaccgg ctcgacgcca tgaatgccct ggcgaaagcc agaggtctga ctctcatcga
      721 tctgctgatg accgcggcac ggctgaaccg cgaggttgtc agacatcttg tcgagtccgc
      781 gcacgctgat gtgaacgccc gggacgcgca tggaatgacc gctgttcacc aggcggcccg
      841 gtacggaaac tactcattca ttcatgaacg agtgtcgcgc ggcgcggtga acggcgccgt
      901 cgagatgatc gaatacctgc tctccgtcgg cgcgcgggtc gacgacccgg ggccgggggg
      961 agccacaccc ctcatgtatg ccgctgagac cgcgaatggc ggcctgatcg actttctgct
     1021 cgggaaaggc gcgaacccgg atgcccgtga tgctcagggg cgctgcgccg ccgagtatgt
     1081 gaaatatccc gaggctccgc ccgaatcgtg ggaaaacgcc tacgaagcct gggggtggga
     1141 atgccacggt ctttccgatc tcgaaaaccg gctcatcaag agcatgcgcc aaccacccac
     1201 cccgcccaaa aagtagggtc caaccgacga ctttcactag gaacagagat gcgtcatctg
     1261 ccgaacgctt caaaacctgc cctgaaaagg acaactctcg gcacggacga gcattatcag
     1321 ttccacaact ccggaaataa cttgtcatgt cgttccttcg tggcttcagc ggtcgatcag
     1381 ccggtacagg ctccctcgac agactcttca gattcgagag gctcgccgtc gcgaaagacc
     1441 tttcaacgcg ctgtcgataa ggtgcggaga atccagagca tccagaagaa cgaatccctc
     1501 gatcgtggca aatgccaacg aggccaatgg tttccggtcc tgttcccgct ccaccttcaa
     1561 gccggaaaca atccattgaa agaatccgtc gcgaatcttt tgggcaatgc cgcgaagaac
     1621 gtcttcgccg ccggcagact gagcgaggtc ggtcggcaat agtggacacc aaaacgttca
     1681 tgcggcgact cgttggtttt cgagaaagaa ctgctcgaat gccgctggtg agagaaaccc
     1741 gagcctggct tgggttcgtt gccgattgca gaagatttcg atgtattcta gtgaattctc
     1801 gagcggaatc cctcatcagg tatgaaccgc tcccacctga aaattctggt tcaggccact
     1861 ttcaaaactt ttacttcctg gtagaggcga tcggcaagcc gatctttttc cacctctaca
     1921 tcaaatcggg tttgcaaatt cagccagaag cgttctgaca atccaaaaaa acgcgccaac
     1981 cgcaaactcg tgtcagccga tactcctcgg atcccgtgga tgatttcatt gatcctgcgc
     2041 cgaggaacgc cgatatcctg agcaaggcga tattggctca atccaagagg ttcaaggaac
     2101 tccttcagca gaatcatccc cggatggatc ggagggagtt ttttggtctt catatgttca
     2161 cctcaatgat aatcagcaac atcaacatcg aatgcgttgc ctgactgcca tcgaaagcag
     2221 actcgccatt gtcggttgat tcgaatactg tattggccag ccctgtttcc agagagtttt
     2281 tccagatgat tggaaggagg aattcgaaga tcctcgatga cctcggcggc atcgagcatc
     2341 tcgagcttca ttcgtgctga ctcgataatc tccgacggaa gacggcgcgc gacctctcga
     2401 tgaaaaagcc gctcggtttc tttgcagcgg aaggacttga tcacgattca aaagtaacgc
     2461 ttcccgttac gcttgtcaac ctgctcatat gaaataccca caaatgtccc catctcaatg
     2521 gaggagtgga ttggggtggg aataaatcca tctcaacccg tcagcaaacg cgatgtctcg
     2581 tttctgaggt gcgcagggat cggatggcgc agcctccagg tgaaactgat cgggcgcgac
     2641 cccttgtgcc cttcgtaatc agcaggaccg aggaagtgga aaggcgccgc gaggccggat
     2701 ggcagcttcc ggttctcgcg gacgaacagg aggatcgttc cgccatgcga gcgatgattg
     2761 atgtatcgct ggcccgtggg agaggagtcg gaggttgtcg actgactctg ccagtgaaac
     2821 agccggtcgc tgatcgcata gtcctcgtac atggtcgtgg gcgaatagtc gctctcgtgc
     2881 ttgttgaggg tgacgaaaaa agcgtcggcg cgaatggtct ggatgtggac aacgccctgt
     2941 cgcacagggg gctggcgcgc gagtgtccag tagccgagac cagacaggat ctcctcgcgg
     3001 atatacgacg aataaagcct cagtggacag ggaaacggga cgtcgagggg cggagcgatg
     3061 gtggcgacct tcgccagacg ataggcgagc agctcgcgga gatcgttgca gaggtgtttg
     3121 ttgccctgca atttggaaag actctccaga aaggagcccg gacggtcgtt tgcgtcccag
     3181 aggacaaaat gcagcatcag cagatgcgct ttctgtccct gcgagagttc gtcggagtgg
     3241 cccggaggaa gatcgacaag agcgaggatg gaggtgatat acgccggatc gtcgatgtgc
     3301 tggagccgtt gaagcccctt ggtgagccgc gcttcgtcag gatcggcaaa atcctcccgg
     3361 attcctgcgg cgacgcacag acgtgaccag gcgcctcggc tgaagatggc atcaggctcc
     3421 agatggtgga attcgaggaa cgccgccagg gatggcgcac gaccggactc attttcgaac
     3481 tcccggattg cttgtacaag cttcggacgc gagtgatcga cggcccgttt caggttatcc
     3541 aagacatagt ggcgtgcctg ccgttcgagg gtgatggtgc agccagccgg gaggtgggca
     3601 aagccgtcgc ggatctgctc ggcgagcgga cgatcggaag ccatcgtcag ggctcgataa
     3661 cgcaagccga ggcggtagcg gtgatgggct tggccgatga aatcgagcac cgtcagacaa
     3721 tccttgtccc tggagagccg caagccacgg ccgaactgtt gcaggaagac cgtcagcgac
     3781 tcggttggcc gcaggaacag cgaagccaga tagtcggcgg ccgcaagtcg agggaatcgc
     3841 gagccccaga actccgcttc tcctcgtggg atcgctacgc cggtcattac acggagaagt
     3901 acacggaaca tgtgatggcg ggcgagtaca gc
//
LOCUS       DAORHD010000319         3927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25172_length_3927_cov_3.545713, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000319 DAORHD010000000
VERSION     DAORHD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25172_length_3927_cov_3.545713"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1395
                     /locus_tag="PKO06_08005"
     CDS             193..1395
                     /locus_tag="PKO06_08005"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNV69623.1"
                     /translation="MLFSATYALGEVPGEIIHYTISAIRLFLIRLFPDNLLIDLLANG
                     LISGIGGVLMFLPNIFFLFCGIAILEESGFFLRSTVLFENFMKWLQLDARALVPLIIG
                     LGCNAPAVMASRLIRNRWQRLRVLFLLPFISCSARLPIAVLLIGTFFPNHPGLWLSGM
                     YACNFLLILALARLTGLYSQETERLPSLGASDLPPYRIPRWKQIWQPVRHHCLHFVEK
                     AGTILLIGTALIWLLQTFPRSSFPQFQEAQLFRNSQERRIDFEESLFISYAGSRNVET
                     SYLGQLGRIIQPLFSPLGFGWRETVALISGVLAKEGIVSTLSVLYGPSNQAISQGMRH
                     NGITPLSALSLLIFSMLYMPCLPTLTLILRESDSTLFTVGMVLFAPTFAYTLAWLIPH
                     ALVMAGLA"
     gene            complement(1456..>3927)
                     /locus_tag="PKO06_08010"
     CDS             complement(1456..>3927)
                     /locus_tag="PKO06_08010"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNV69624.1"
                     /translation="PRTFWHIALEVAQEPMFLLLVTCGVLYFILGDPEEAMMLLGFVF
                     VVMGITFFQERKTERALDALRDLSSPRALVIRSGEQIRIPGREVVPGDVLVLAEGDRV
                     PADGAVIDSLNLSLDESLLTGESVPVRKIAAVEPVPQSMGKPGGDDLPFVYSGTMVVQ
                     GQGYAKILATGMKTELGKIGKALQNVETEESTLKVETGRLVRHLAILGFFSCLLVIVA
                     YGLTRGNWTEGFLAGITLAMAMLPEEFPVVLTIFLALGAWRISQKQVLTRRVAAIETL
                     GAATVLCSDKTGTLTQNRMTVVRLCTDGAEIVLAPGSTNALPEQVHCLAEFAILASQT
                     DPFDPMEKAIRQVGFDHLANTEHLHDNWSLVREYPLSRELLAISRVWKSPEGERFIIA
                     AKGSPEAIADLCHLSNEQKGELEKRINHLAESGLRIIGVASASFSAENLPSNQHDFSF
                     EFIGLLGLEDPIRPTVPASVVECQGAGIRVVMITGDYPGTAQNIARQIGIAQPQNVLT
                     GQELDILSPEELAERVKSVNIFARIVPEQKMKIVQALKQNGEITAMTGDGVNDAPALR
                     SAHIGIAMGARGTDVAREASALVLLDDNFTSIAAAVKLGRRIFDNLQKAMSYIFAIHV
                     PIAGLSLIPVLMKWDLILLPMHIVFLELLIDPSCSVVFEMEPEESDVMNRPPRPRDQP
                     LFGRETLVISLIQGLAVLAFVLGVFLIGKFHLKFPVNECRALAFTTLIIANICLIIVN
                     RSHTRLAMETLMTPNPAQWWVVFGGLFFLLLVLRVPFLMQMFKFEYLHINDIAICFGA
                     GVGSLIWFEGFKAAFRRKVQKSPSQ"
BASE COUNT          902 a         1131 c         1007 g          887 t
ORIGIN      
        1 ccggcacagc caggaagtca tagtccctga ttttatgggc aacatcttct tgcctgccga
       61 gacaatgccc tgagagtgtt tcaggatcga attgtctcga aagtacaacc tcaaacatct
      121 tctcaggttc ttctggaagg atatctccat catcagtttt ggggaatacc agcgttcttc
      181 gcggtgatgg gtttgttgtt ctccgcgacc tacgcactgg gagaagttcc gggcgaaatc
      241 attcactaca cgatctctgc cattcgtctg tttctgatac gcctgtttcc tgacaacctg
      301 ctgattgatc ttctggccaa tggcttgata tcggggatag gcggagtact gatgttcctt
      361 cccaacatct tttttctgtt ctgcggcatt gccattctcg aagaatccgg gtttttcctg
      421 cgctcaaccg tcctattcga aaacttcatg aaatggcttc aactcgacgc tcgagcactc
      481 gtgccactca tcatcggact gggatgcaat gctccggcgg ttatggcctc acgattgatc
      541 cgtaaccgct ggcaacgcct cagagtcctg tttctcctgc cgttcatcag ttgctcggca
      601 cggttgccca tcgccgttct tctcatcggg actttctttc cgaatcaccc cggcctctgg
      661 ttatcaggca tgtatgcctg caacttcctc ctcatcctcg ctctcgctcg cctcactggc
      721 ttgtactcgc aggaaacgga acggcttcca tcgttaggcg cgtccgatct tcctccctac
      781 cgcatcccaa ggtggaaaca gatctggcaa ccggtgcgac accattgcct gcacttcgtc
      841 gagaaagcag gcaccatcct tctgataggc acagccttga tctggctcct tcagacattc
      901 ccacgatcca gctttcccca gtttcaagag gcacaactct ttcggaactc acaggagcgc
      961 cgtatcgatt tcgaggaatc cctcttcatt tcctacgcag ggagcagaaa cgtagaaacg
     1021 agctaccttg gccaactcgg acgaatcatt caaccgttgt tcagtccgct cggattcggt
     1081 tggagagaaa cggtagccct gatttccggg gttctggcga aagaggggat tgtttcgacg
     1141 ctgagtgttc tatacgggcc atcgaaccaa gccatttctc aaggtatgcg ccacaatgga
     1201 atcactccgt tgtcggctct ctccttgctc attttctcga tgctgtacat gccttgcctg
     1261 ccaaccctga cactgattct ccgcgaatct gattcgacac tgtttaccgt cggaatggtc
     1321 ctctttgctc cgacattcgc ctacaccctg gcctggctca ttccgcacgc actggtaatg
     1381 gccgggcttg cttagctcag ctcgtgcgga gtggccagga gcttcatttc ggacggggac
     1441 cgacagggga agacgttact gactcggact tttctgaact tttcggcgaa atgccgcctt
     1501 gaatccctcg aaccagatga gactgccaac tcccgctccg aaacagatgg cgatgtcatt
     1561 gatgtgcaga tactcgaatt tgaacatctg catcagaaac gggactcgaa gaacgaggag
     1621 cagaaagaac agcccgccga ataccaccca ccattgtgcc ggattgggtg tcatcagcgt
     1681 ttccatggca agacgcgtgt gcgaacgatt cacgatgatc agacagatgt tggcgatgat
     1741 caaggtagta aaggcgaggg cccgacactc gttcaccgga aacttgagat ggaacttgcc
     1801 gatcaggaac accccaagaa cgaaagccag aaccgcgaga ccctgaatca gactgatgac
     1861 gagggtttcc ctgccaaaca acggttgatc gcgaggccgt ggaggacggt tcatcacgtc
     1921 ggattcttcg ggttccattt cgaacaccac tgaacaggaa gggtcgatca gcagttcgag
     1981 aaagacgatg tgcatcggca ggaggatcag atcccatttc atcagtacgg gaatcaggga
     2041 aagaccggcg atgggcacat gaatggcaaa gatatagctc atcgctttct gcagattgtc
     2101 gaagattcgc cttcccagct tcacggccgc agcgattgac gtgaaattgt cgtccagcag
     2161 taccagagcc gacgcctcac gcgcgacatc ggtgccgcgc gcacccatgg caataccgat
     2221 gtgtgctgac cgcagagcag gagcatcgtt gactccatca ccggtcatgg ccgtgatttc
     2281 cccattctgc ttcaacgcct gaacgatctt cattttctgt tcaggaacaa tacgcgcgaa
     2341 gatgtttacg ctcttcacgc gctccgcaag ctcttcagga gacaagatat cgagttcctg
     2401 cccggttaag acattttgcg gttgagcgat accgatctgc cgcgcaatat tctgagccgt
     2461 accgggatag tcgccggtga tcatgaccac ccggattccg gccccctggc attcaacaac
     2521 cgacgccgga acggtcgggc ggatgggatc ttcgagtccc agtaacccga tgaactcgaa
     2581 cgaaaaatca tgttgattgg aaggcagatt ctcagccgag aacgaggcgg atgcaacacc
     2641 gatgattcgc agcccggatt cggccaagtg gttgatgcgc ttctccagct cccctttctg
     2701 ctcgttgctc agatggcaaa gatcggcgat cgcttcaggc gaacctttcg cggcaatgat
     2761 aaatcgctcc ccctcaggcg atttccagac gcgagaaata gcgagaagct ccctggacaa
     2821 tggatactct cgaaccagac tccagttatc gtgaaggtgc tcggtgttgg ccaaatggtc
     2881 gaagccgacc tgacgaatgg ctttttccat gggatcaaac gggtcagtct ggctggcgag
     2941 aatggcaaac tcagccaggc aatgaacctg ttcgggcaga gcgtttgttg aaccgggcgc
     3001 cagtacaatc tcggctccat cagtgcacaa tctgacgacc gtcatgcggt tctgggtcaa
     3061 ggtgccggtt ttgtcactgc acagcacggt cgcagccccc agagtctcga ttgcggcaac
     3121 gcggcgcgtg agtacctgtt tctgagatat ccgccaggct ccaagcgcaa gaaagatggt
     3181 cagaacaacc ggaaactctt ccggaagcat ggccatcgcc agcgtgatac cggccagaaa
     3241 gccttcggtc cagtttccgc gcgtgaggcc gtaggccacg atcaccagga gacagctgaa
     3301 aaacccgagg atagccaaat gacgtacgag tcgcccggtc tcgactttca gtgtcgattc
     3361 ctcagtttcg acgttctgca gagctttccc gatcttgcca agttctgtct tcatgcctgt
     3421 tgcaaggatc ttggcatacc cttgtccctg cacgaccatc gtgccagagt agacaaacgg
     3481 caagtcatct cctcccggtt ttcccatcga ctgcggaacc ggctccacgg ccgcgatttt
     3541 acgaaccgga accgattcgc cggtcaagag cgattcatcc agagacaggt tcagagaatc
     3601 gatgaccgcc ccatccgcgg gaacgcgatc tccttctgcg agaacgagaa catcgcccgg
     3661 caccacttcc cgaccgggaa tacgaatctg ctcaccgctg cgaatcacaa gggcccgcgg
     3721 gcttgagaga tcgcgcaatg catcgagagc tcgctccgtc tttcgttcct gaaagaaggt
     3781 gattcccatg accacgaaga cgaaaccaag aagcatcatc gcctcttcag ggtcgccaag
     3841 gatgaaatac aagacaccac aagtgacgag aagcaaaaac atcggttcct gagcaacttc
     3901 gagggcaata tgccagaaag tgcgagg
//
LOCUS       DAORHD010000320         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25184_length_3926_cov_3.572204, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000320 DAORHD010000000
VERSION     DAORHD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25184_length_3926_cov_3.572204"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PKO06_08015"
     CDS             <1..256
                     /locus_tag="PKO06_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69625.1"
                     /translation="EGKEIARVEEKPDEFGRSLLEVELSAEGAAPFETFTRLHVGKQA
                     AIVDNGSTVISAPVIQSVISGGRLQIYGMTAKDLLPATKP"
     gene            complement(264..1217)
                     /locus_tag="PKO06_08020"
     CDS             complement(264..1217)
                     /locus_tag="PKO06_08020"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HNV69626.1"
                     /translation="MKRTIIIWSLAFLSLVGSTVSAGGLVPGTASPIVSLKSLSDAPE
                     AERLIRAALRIAPERRLAFFSRCLLGRRYHPETKKRISGQRQQPVTKREATNPSPLPV
                     THLATSLAFLDCMTYVEHVLALTFCEKPEYTGCFLPRLIDLMFDAEGGPLFNHQRNHF
                     TSLWAITNARKGYLVDVAAKHPLAVRRTLTLNRVADNRTFYVEDRFMIAPGPTSFAYF
                     PLSVVTGRRAPLESGDIVALVCDKEGLDVTHMGFFMQPHEKPVLRHASLAKNRIVDED
                     FYAYVQAKKGLVGLMVLRPCLPPGRPVNLYRFDLLPQPAKP"
     gene            1299..2036
                     /locus_tag="PKO06_08025"
     CDS             1299..2036
                     /locus_tag="PKO06_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV69627.1"
                     /translation="MDHTRFSIVFLDEWYVAIEKPVDWLIHHSPTAPRSQPAVVPVLE
                     AQLGRKVFPVHRLDRATSGLLLFALSSEAAAAVAMQFEQRQITKTYLAVVRGWFAPPA
                     GSIERPLSPMPGENQQEARTCYETVAQCELPVPIPPHQTCRYSLMRVFPETGRRQQIR
                     RHFAGAAHPIIGDGHHGDRRHNQFFAERFGCRRMLLMAARLKFYHIWLKRFLELQCHL
                     DEPVQALLSDLFPGSMGDEPSQGEREP"
     gene            2095..2268
                     /locus_tag="PKO06_08030"
     CDS             2095..2268
                     /locus_tag="PKO06_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNV69628.1"
                     /translation="MKKIGKRGQGLVEYGLVIALISLVCIALLTGIGGGVADDYDQVS
                     TELNHVETSMTGW"
     gene            2566..>3926
                     /locus_tag="PKO06_08035"
     CDS             2566..>3926
                     /locus_tag="PKO06_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV69629.1"
                     /translation="MSNESNTGVDFQDFALSEPIMKALRDVGYEKPTPIQEQVIPLVL
                     EGRDVVGQAQTGTGKTAAFALPLLTKLDLKCLAPQVLVLTPTRELAIQVAEAFHTYAA
                     HLKGFQVLPIYGGAEYGGQLRGLRRGAHVIVGTPGRVMDHLERETLSLETLSCLVLDE
                     ADEMLRMGFKEDVEWILDHTPAERQISLFSATIPPQIRALARKYLREPIEITIKSRTT
                     TVETTRQRYWLVSGLHKLDALTRILEAETYDAILIFVRTKIATLELSERLAARGFAAA
                     PLSGDIAQTQRERTIEQLRSGKLNIVVATDVAARGLDVDRISHVINYDIPYDTESYIH
                     RIGRTGRAGRTGEAILFVAPKERRILQVIERATRQPITLMELPTSELINDRRIAQFKQ
                     RVTETLAEKDLGFFRTLLEAYTLEQGLEPIDVAAALAHLYQGETPMLLPPTPPPQPAM
                     SDRP"
BASE COUNT          829 a         1081 c         1210 g          806 t
ORIGIN      
        1 cgaggggaag gagattgcgc gtgtcgagga gaaacccgat gagttcggcc gcagtctgct
       61 ggaggtcgaa ctgtcagccg agggagccgc acctttcgaa accttcacgc gtttgcacgt
      121 gggcaagcag gcagcgatcg tggacaatgg cagcacggtg atttctgctc ctgtcattca
      181 gtcggtgatt tccgggggta gactgcagat ctacggaatg acagcgaaag acctgctccc
      241 cgcaacaaaa ccgtgaatca cgctcagggc ttcgccggtt gaggcagaag atcgaatcgg
      301 tagaggttca cggggcggcc ggggggcagg catgggcgca acaccatcag gccgaccaat
      361 ccctttttag cctgcacata ggcgtagaag tcctcgtcca caatccggtt tttcgccagc
      421 gaggcatggc gaagaaccgg cttctcatgc ggctgcatga agaagcccat gtgtgtgacg
      481 tccaggcctt ccttgtcgca gaccagagcg acgatatcac cggattcaag aggtgcgcgg
      541 cgccccgtta cgactgaaag cgggaagtag gcgaaggaag tcggccctgg ggcgatcatg
      601 aaacggtctt cgacatagaa cgtccgatta tcggccacgc ggttcagcgt cagggtgcgg
      661 cgcaccgcca atgggtgctt tgccgcgaca tcgaccagat accctttacg cgcgttggtg
      721 atcgcccaca gtgaggtgaa gtggttgcgt tgatggttga acagggggcc gccttccgcg
      781 tcgaacatga gatcgatcag gcgtggcaga aagcagccgg tgtattctgg tttctcgcag
      841 aaagtcaggg ccagcacatg ttcgacgtag gtcatgcagt cgagaaaggc gagactggtg
      901 gccagatgcg tgaccggcag gggcgaggga ttcgtcgctt cccgcttggt cacaggttgt
      961 tgtcgctgcc cggagatgcg cttcttcgtt tcagggtgat accgtcgccc gagaaggcag
     1021 cgggaaaaga aggccagacg ccgttcaggg gcgattctca gcgcggcacg aatcaggcgc
     1081 tcagcttcgg gggcatctga aagagacttg agcgatacga tcgggctggc cgtgccgggg
     1141 acgagtccgc cggccgagac ggtagaaccc acgagagaaa ggaatgccag tgaccagatg
     1201 atgatggttc ttttcatgtc ctcaaacata gcgtattctg gccgtcgctg caccactttg
     1261 acgtgcaacg gaactgaccg gctgaggcgt gctgaccgat ggatcatacc cgtttctcga
     1321 tcgtgtttct cgacgaatgg tatgtcgcga tcgaaaagcc ggtcgactgg ctgattcacc
     1381 attcgcccac ggcgccacgc agtcagcccg cggtggttcc ggtgcttgaa gcccaactgg
     1441 gccggaaagt gtttccagta catcgtctgg atcgcgcgac ctcgggtctg ctgctgtttg
     1501 ctttgtcttc agaagcagcc gcagcggtcg ccatgcagtt cgagcaacga cagatcacca
     1561 agacgtatct ggccgtcgtg cgaggctggt ttgctccgcc cgccggcagc atcgagcggc
     1621 cgctcagtcc gatgcccgga gaaaatcaac aggaggcgcg aacgtgctat gaaacggttg
     1681 cacagtgcga gcttccggtt ccgatccctc cccatcagac ctgtcgctac tcgctgatgc
     1741 gggtgtttcc tgagacgggg cgacggcagc agattcgtcg acattttgcc ggcgcggcgc
     1801 atccgatcat cggtgacggg catcacggcg atcgtcgcca caaccagttt ttcgcggaac
     1861 ggttcgggtg tcggcgcatg ttgttgatgg ccgctcgtct gaagttctat catatctggc
     1921 tgaaacgttt tctggaactg caatgccacc tggacgagcc agtacaagcc ctcttgagtg
     1981 acctgttccc cgggagcatg ggagacgaac cgtcacaggg agagcgcgaa ccctgaattg
     2041 gcactaaaaa tgcgttttta actgtacgac gatttataag cggaggataa gagtatgaag
     2101 aaaatcggaa aacgaggtca gggtctggtg gagtatggat tggtgatcgc cctcatttcc
     2161 cttgtctgca tcgccctgct cacaggtatc ggtggcggtg tcgccgacga ttacgatcag
     2221 gtttcgaccg agttgaacca tgtggaaacc tcaatgacag ggtggtgagt gcgacggtcg
     2281 gggctgaggc aaattgaccc cgggttgacc cgcaaatacc cgaagcattt cggtacaaat
     2341 cccgccttat ctctcacgag agggcgggat ttttctctct ctgaaggaat cagattcatg
     2401 ctagaatgtc ggggtcagga aatttcttcc aggcattgtt tgccgatcaa gtcataatgg
     2461 cgcacttcac agcgactgca tgaaccagac gacatctgca gatgattcgc ttccggcttg
     2521 gcgataaggc cggttttcgt cgtcccccac cataaggagc aacgcatgtc gaacgagtca
     2581 aacaccggtg tggattttca ggattttgcc ctctctgagc caatcatgaa agccctgcga
     2641 gacgtagggt atgagaaacc caccccgatt caggagcagg tcattcccct ggtgctcgaa
     2701 gggcgcgatg ttgttggtca ggcccagacc ggcacaggga aaaccgcggc cttcgccctg
     2761 ccactgctga cgaaactcga tctcaagtgt ctggccccgc aggtgttggt gctgaccccg
     2821 acccgcgaac tggcgattca ggtggccgaa gcgtttcaca cctatgcggc gcatttgaag
     2881 gggtttcagg tgttgccgat ctacggtggc gcggaatatg gcggacagtt gcgcgggttg
     2941 cggcgtgggg cgcatgtgat cgttggtact cccggccggg tcatggatca cctggaacgt
     3001 gaaaccctct cgctcgagac gctttcctgt ctggttctcg atgaggctga cgagatgctg
     3061 cgcatgggct tcaaggaaga tgtggagtgg attcttgatc acacgcccgc ggaacggcag
     3121 atctcgctgt tctcggcgac gattccgccg cagattcggg ctttggcacg aaagtacctg
     3181 cgagaaccga tcgagattac gatcaaaagc cgaacgacga ccgtggaaac cacgcggcag
     3241 cgctattggc tggtgagcgg tctgcacaag ctcgatgcgc tgacccgcat tctcgaggct
     3301 gagacctacg atgccattct catctttgtt cgcacgaaga tcgccacgct cgagttgtct
     3361 gaaaggctgg cagcccgcgg atttgcggca gcgcctctga gtggagacat tgcccagact
     3421 caacgcgagc ggaccatcga acagttgcgt tcggggaagc tcaacattgt ggtggccacg
     3481 gatgttgctg cccgaggtct tgatgtcgac cgcatcagcc atgtcatcaa ctatgacatt
     3541 ccgtatgata ccgaaagcta cattcatcgc attggccgca ccggccgggc cggccgcacc
     3601 ggggaagcca ttctgttcgt cgcgccgaaa gagcgccgca ttctgcaggt gatcgaacgg
     3661 gcgacccggc agccgatcac cttgatggaa ctgccgacat cagagctgat caacgatcgg
     3721 cggatcgcgc agttcaagca acgggtgacc gaaaccctgg ctgaaaaaga cctgggcttc
     3781 tttcgcacgc tcctggaggc atacacgctc gaacaagggc ttgaaccgat cgatgtcgcg
     3841 gcggcactgg ctcatttgta ccagggagaa acgccgatgt tgcttccccc gacgccgcct
     3901 ccacagccgg caatgtcaga tcgccc
//
LOCUS       DAORHD010000321         3920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25257_length_3920_cov_3.861320, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000321 DAORHD010000000
VERSION     DAORHD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25257_length_3920_cov_3.861320"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PKO06_08040"
     CDS             <1..931
                     /locus_tag="PKO06_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69630.1"
                     /translation="LGSRVDPAWYNNMLVDRPGANYLLPARLVQRVGNAVARTTAYRL
                     TDLDVGHPAFRLFSAEENGDPGRAQIFEFFETEPNTSAMILAKLSHGLPAIIEERRGQ
                     GRVVLVTFNADTSWSDWPLKPTYLPFLHQTLFGLVSKAGLSRDSVVPGTPVLLTLRSQ
                     GLRRVVLTPPNGRSQEFAPPADNGPIAHFSFTSTEEAGIYKVLIEQTEGSRVEAFTVN
                     PPAEESDLQRFELNKIPRFIPLQPRPGDKEKLGDRIALVREGRELAGTLLLLLLLLAL
                     IETFLANRAPTPLTGGLLRAKPESPTDTGVSSA"
     gene            928..3201
                     /locus_tag="PKO06_08045"
     CDS             928..3201
                     /locus_tag="PKO06_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNV69631.1"
                     /translation="MKTLISLLGLEIGALQQLESIRLGFVWGWAEILLLALLFVPVII
                     WFYRLEEKPHTPSFRRLTLGLRLIFVIAILLLLAGPRFLVTGFIPQKNKIAVLVDASR
                     SMSIVEEGKARIDRARDTLSQGRFLAKLEKQTELPPSLFSFAGQVSPLTADDISSFSV
                     TPDGLQTNLTRAVTEVVSNLGESNLLGVILCTDGGHNTGDSPREALAGLRTPLYILGT
                     GRANQARDLSISLERPPALGYLNSRVRVRGEVRLYRIATDTIPITIKRDGKPYEQIVL
                     TVGSDSRRLPFSCNLPCDEEGAFTWTFSVPQVAGELTFDNNEAGFLLRVIKDRMRVLL
                     LAGHADWDHTFLRQALQSDPSAQFNALVRVNEQRWLRLENFVLKGPITTPDIGSETAD
                     IDLLILAGISETLLRPHAENLTRRLESGQLGVLLLPAARGYAALGYRDSPLARWFPVE
                     LTSETWRGVPTSLLLPTREPAFGFLRLLDDPLQNQEFFQNTIPKFDGLFTYGRLKTGS
                     QILLSSTLDNGGTPAPALISQRLGQGQVAMLTGGSLWPMGLRLVATGKGNRPYTAFTV
                     NLLKWLANRREDSQVVLEIAAARGFVNQPTSIRAWVYDAARRPLETAQIVGTASGHEG
                     ETLPLSFLAAAEKGCYETAFIPPRKGKFEIRVEARSQGQSLGEARAQFLVEQSTAEFD
                     NPEVQVELMQSLASMTGGVYRPAEESDQLLAQIKATPGKKRETRILELRDSWVLLALL
                     LLFPLSEWILRRTRGFS"
     gene            3201..3899
                     /locus_tag="PKO06_08050"
     CDS             3201..3899
                     /locus_tag="PKO06_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HNV69632.1"
                     /translation="MPRHLLRTLSRLLLTMISLVLLCPIVPANAQASWGTIRIPRIQY
                     GGGGDWYSDPTSIPNLLKAAAERLQLPTRPENLSLKILDAELFHYPMLYLTGHGNVRF
                     EEAEVSRLQSWLDNGGFLWIDDCYGLDKSIRRELRRVYPDKELAPLPTDHPIFHTAYD
                     LPKGLPKVHEHDGKPPVAYGIFNKGRMVVLYTYETDIGDGIEDPGVHPEDSPEIREEA
                     MKMALNILLFAMTQ"
BASE COUNT          743 a         1274 c         1127 g          776 t
ORIGIN      
        1 tctcggctct cgcgttgatc ctgcctggta caataatatg ctggtcgatc gaccgggcgc
       61 gaactatctg ctacccgccc ggctggtaca gcgggtcggc aatgccgtcg cccgcacaac
      121 cgcctatcga ctgactgatc tggacgtggg acacccggcg ttccgactgt tttcggctga
      181 agaaaacggc gacccgggtc gcgctcagat tttcgagttt ttcgaaaccg agcccaatac
      241 gtcggcgatg attctggcca agctcagcca cggtctgccg gccatcatcg aggagcggcg
      301 cggacagggg cgggtcgtac tggtcacgtt caacgccgac accagctgga gtgactggcc
      361 gctgaaaccg acgtatctgc cttttctgca ccagacattg ttcggcctgg tcagcaaagc
      421 cgggctcagt cgcgactcgg tcgttcccgg aactcccgtg ctgctgacgc tgcgcagtca
      481 ggggctcaga cgcgtcgtat tgactccgcc caacggcagg agccaggagt ttgcccctcc
      541 tgccgacaac gggccgatcg cgcatttctc gttcacctcc accgaagaag ccggcatcta
      601 caaagtcctc atcgaacaga ccgaaggcag ccgcgtcgag gcgttcacgg tcaatccgcc
      661 agccgaagag agtgatctgc agcgcttcga gctgaacaaa attccgcggt ttattcccct
      721 gcaaccacgg ccgggcgaca aagagaagct cggtgaccgc attgccctgg tacgggaagg
      781 gcgcgagctt gccgggaccc tgctgctgct cctgctgctg ctcgccctga tcgaaacatt
      841 cctggccaac cgggcgccga cacccctcac gggcgggttg ctgcgcgcca agcccgagag
      901 tcccaccgac accggagtca gcagcgcatg aagacgctca tcagcctgct gggattggaa
      961 atcggtgcgc tgcagcagct cgagtcgatt cgcctcggct tcgtctgggg atgggccgag
     1021 attctgctgc tcgccctcct gtttgtccca gttatcatct ggttctatcg gctcgaggaa
     1081 aagccgcaca cgccttcgtt tcgtcgcctg acccttggcc tgcgtctcat cttcgtcatt
     1141 gccatcctgc tgcttctcgc tggcccgcgt ttcctggtga ccggcttcat tccgcagaaa
     1201 aacaagattg ccgtgctggt cgatgcttcg cgctcgatga gtatcgtcga agaaggaaaa
     1261 gctcgtatcg accgggcgcg cgacaccctg tcccagggtc gatttctcgc aaaactcgag
     1321 aagcagacag aactgccgcc cagtctgttc agcttcgccg gacaggtgtc accgctgact
     1381 gctgacgaca tcagctcgtt ttccgtcacc ccggacgggc tgcagaccaa cctcacccgc
     1441 gccgtcaccg aagtggtcag caatctcggc gaaagcaatc tgctcggcgt cattctctgc
     1501 accgatggcg ggcacaacac tggcgactcc ccgcgtgaag ctctggctgg tttgcgcacc
     1561 ccgctctaca ttctgggtac cggtcgcgcc aaccaggccc gcgatctgtc gatcagtctc
     1621 gaacgccctc cggctctggg ttatctcaat tcccgagtcc gggtgcgcgg cgaagtgcgt
     1681 ctgtatcgca tcgccaccga cacgattccg atcaccatca agcgcgacgg caagccctac
     1741 gagcagatcg ttttgacggt cggcagcgac agccgtcggc tgccgttctc ctgtaatctg
     1801 ccctgcgacg aagagggggc cttcacctgg accttcagcg tgccgcaggt ggctggtgaa
     1861 ctcacctttg acaacaacga ggccgggttt ctgctgcgcg tgatcaagga ccgcatgcgc
     1921 gttctgctgc tggcaggtca cgccgactgg gaccacacgt tcctgcgaca ggctctgcag
     1981 tcagacccga gcgcacagtt caacgctctg gtgcgggtga acgagcaacg ctggctgcgc
     2041 ctggaaaact tcgtgctgaa agggccgatc accacgcccg atatcggctc ggaaaccgcg
     2101 gacatcgatc tgctgattct cgccgggatc agcgagacgc tgctgcggcc gcacgcagag
     2161 aacctgacca ggcgtctgga aagcggacaa ttgggcgtgt tgctgcttcc ggccgcccgc
     2221 ggctacgccg cgctggggta tcgcgattca ccgctggcac gctggtttcc cgtggaactt
     2281 acatccgaaa cctggcgcgg cgtgccgacc agcctgttgc tgccgacccg cgagccggca
     2341 ttcggatttc tgcgtctgct cgatgatccg ttgcagaacc aggagttttt ccagaacacg
     2401 attccgaagt tcgacggcct gttcacttac ggccgcctga agaccggctc tcagattctg
     2461 ctctcttcga cgctcgataa cggcggcacc ccggcaccgg cactgatctc tcagcgactc
     2521 ggccaggggc aggtcgccat gctgaccggg ggttccctgt ggccgatggg cttgcgtctg
     2581 gtggccaccg ggaaaggcaa tcgcccttat accgccttca ccgtcaacct gctcaaatgg
     2641 ctggccaatc gacgcgaaga ctcgcaggtt gtgctcgaga tcgcggcggc acgaggtttc
     2701 gtgaatcagc cgacttcgat tcgcgcctgg gtatatgatg ccgccagacg acctctcgaa
     2761 accgcgcaga tcgttggcac ggcgtctggt cacgagggcg aaacactgcc gctgagtttt
     2821 ctggccgccg ccgaaaaagg gtgttacgag accgctttca ttcccccacg caaaggcaag
     2881 ttcgagattc gggtcgaggc ccggtctcag gggcagtcac tcggtgaagc ccgggcccag
     2941 tttctcgtcg aacagagcac cgccgagttc gacaaccccg aagtgcaggt tgagttgatg
     3001 cagtcgctgg ccagcatgac cggcggcgtc tatcgcccgg ccgaagagtc tgatcaactg
     3061 ctcgcccaga tcaaggccac accgggcaaa aaacgggaaa cccgcattct tgaactgcga
     3121 gacagctggg tgctgctggc tctgctcctg ttattccccc tgtccgagtg gatcctgcgc
     3181 cgaacgagag gtttcagctg atgccacgcc acctgttgcg aacactcagc cgcctgctgc
     3241 tgaccatgat ttctctggtt ctgctgtgtc cgattgtccc agccaatgct caggcatcct
     3301 ggggaacgat ccgcattccc cgcattcagt acggcggtgg tggcgactgg tactccgatc
     3361 ccacgtccat tcccaacctg ctcaaagccg ccgccgagcg cctgcagctg ccgacgcgcc
     3421 ccgagaatct ctcgctcaag attctcgatg ccgaactgtt ccattatccc atgctgtatc
     3481 tgaccggtca cgggaacgtc cgattcgaag aggccgaggt ctcacgcctg cagagctggc
     3541 tcgacaacgg cggttttctc tggatcgacg actgctacgg tctcgacaag tcgattcgtc
     3601 gcgaactgcg gcgggtgtat cccgacaagg aactcgcgcc tctcccgacc gatcatccga
     3661 tctttcacac cgcgtatgat ctgcccaagg ggctgcccaa ggtccatgaa cacgatggca
     3721 aaccgcctgt agcctatggt atcttcaata agggtcgcat ggtggtgctc tacacctacg
     3781 aaaccgacat cggcgacgga atcgaagatc ccggtgtcca cccggaagac tcgcccgaga
     3841 ttcgggaaga agccatgaag atggcactca acatcctgct ttttgcgatg acacaatgaa
     3901 ccggaatctg cacccaaacg
//
LOCUS       DAORHD010000322         3919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25271_length_3919_cov_4.229814, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000322 DAORHD010000000
VERSION     DAORHD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25271_length_3919_cov_4.229814"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /locus_tag="PKO06_08055"
     CDS             complement(<1..2019)
                     /locus_tag="PKO06_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69633.1"
                     /translation="MRTMIACCLSLVLACPAYADLAAARAAFDKKDFATALKEVQPLA
                     DKNEANASLMLGLMFADGKGVKRNKDEAIRLITAAIQGKADLSLRLPESHNALTWAID
                     TNKPELIEQLLQAGADINLPGYDDRSPLMTAVKEGRVELVQKLLERGARLDQTDREGN
                     LPLHMAAGIYTFEKAKTIIDLLLPKCNINAVNKEGESALLEALDNGKKDIALYLVEKG
                     ADINLADTEGEAPIFCVMDDFEPAEALELLKVLAPKGLKFEAINKYGKTVLHELARSA
                     THQEALEKLKALDEVGAVSKLVNAADSDGLTALMFAAQERSDDGSPVPMVTLLLEKGA
                     DLKAISKRGNAVLHLAIDPRTPEVSLQLLDLLLARGIDVNQPGEKKMTALHMLAEAAE
                     RPGATVIAKRLLEKGANPNLLDENGRTPLHIAADNRDKPELGLFAQALLDGKADPNLL
                     DQEKRAPLHIAAREGGKDFVRVLLSKGADPKLADKYRDTPEKIAEENGNKEIYAMIRD
                     QVNPAPAAAPAGSGPLSKADQEKRATELYEYLAKISRNDIATIEKTMWEIVDRCPETT
                     QAPDALWKLSNLYLTGKDQPDYDNAQKALEKLAATYPQFQPSLPLESALPHVQKRLGF
                     IYEKKENWDKVIALYEPLFMDAGKLSNGEYVGYGLTLGGIYEKKGMKDK"
     gene            2143..2679
                     /locus_tag="PKO06_08060"
     CDS             2143..2679
                     /locus_tag="PKO06_08060"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV69634.1"
                     /translation="MRSSLFCALLGICVILLAGCTGGGGGSDPVVTTIGVGSVAPEIA
                     AGKWYNSGDQPVTLTAQKEAGKPVVLSFCRSDCHVCKGQMPALVGLYNQHKDKAVFIT
                     LSDEETGPIEKCLQDFAAVQWAVGAKSETYKAYDVEYVPTYVLIDKSGTIRHMGHYAV
                     DGATDVDDLETAIEEFVK"
     gene            complement(2788..>3919)
                     /locus_tag="PKO06_08065"
     CDS             complement(2788..>3919)
                     /locus_tag="PKO06_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69635.1"
                     /translation="ILKQAYQDEFLADEAANLLGQAHEALGNRSEAARMHIVASMRPY
                     AGVQVQEDSKAALKRMNVTMEEIRVWDKEWRQAARPLAKQPQKKDPFTASIQVGVEAG
                     DNIPAIADELAPFVPRRADTGKFWNVDLGYNFRDDKHQRIDLDVGYSGNDYHHLNQFE
                     TDSINALLAWRVHGKNHQWRFVPSIGRMELGDRDYLHNWGMEVGYVHRQATGRWLDAN
                     VRGRWNDFQIAPRLAREDRDGRDTRLSISQNWRETTRSGLSSTYGIGLFAARADTDGA
                     DYDTDKRGLRLFLRRDLNRKLTFYGSYTYEDRDHDNASVRSVTGQSRHDKDHQTFMRL
                     DHRFDKKQSVFATYQDSLNDSNLGTSFGFRARTWSLGYSHDF"
BASE COUNT          697 a         1077 c         1229 g          916 t
ORIGIN      
        1 cttgtctttc atgcccttct tctcgtagat gccgccgagg gtcaggccgt agccgacata
       61 ttctccattg ctgagcttgc cggcgtccat gaacagcggc tcgtacagcg cgatgacttt
      121 gtcccagttc tctttctttt cgtagatgaa tccgagacgt ttctgcacgt gtggcaaggc
      181 actctcgagg ggaagagacg gctgaaactg cggatacgtg gcggccagtt tctcgagtgc
      241 cttctgggca ttgtcgtagt cgggttgatc cttgccggtc agatacagat tcgagagctt
      301 ccagagtgcg tcgggagcct gcgtcgtctc cgggcaccga tcgacgatct cccacatcgt
      361 tttttcgatc gtggcaatgt cgttgcggct gatcttcgcc agatactcgt acagttcggt
      421 cgctcgcttt tcctgatctg ccttgctcaa cggaccagaa ccagccgggg cggccgcggg
      481 cgccggattg acctgatcgc ggatcatggc gtagatttcc ttgttgccgt tttcctcggc
      541 gatcttttca ggtgtgtctc gatatttgtc cgcgagcttc ggatcagctc ccttgctgag
      601 aagaactcgg acaaaatcct tgccgccctc tcgcgcggcg atgtgcaggg gcgcgcgctt
      661 ttcctggtcg agcaggttcg ggtcggcctt gccgtcgagc agggcctgcg cgaacaaccc
      721 gagttcaggc ttgtcgcggt tgtcggcagc gatatgcagg ggggttcgcc cgttttcgtc
      781 gagcaggttc gggttggctc ccttttcgag cagtcttttc gcgatgacgg tggcgcccgg
      841 gcgttcggcc gcttcggcca gcatgtgcag ggcggtcatc ttcttctcac cgggctggtt
      901 cacgtcgatg cctcgggcga gcaacagatc gagcagttgc agactgactt ccggcgtgcg
      961 cggatcgatc gccagatgca gaacagcgtt tccgcgtttc gaaatggcct tgaggtcagc
     1021 ccccttttca agcagaagag tcaccatggg taccggtgag ccatcatcag atcgttcctg
     1081 cgctgcgaac atgagggcgg taagaccatc actgtcggcg gcgttcacca gtttgctgac
     1141 ggcgccgacc tcatcgaggg ccttgagctt ctcgagtgct tcctggtgtg tggcgctgcg
     1201 ggcgagttca tgcagaaccg tcttgccgta cttgttgatc gcctcgaact tgaggccttt
     1261 cggagccagg actttcagaa gttcgagagc ttctgctggc tcgaagtcgt ccatcacgca
     1321 gaagatcggg gcttcgcctt ccgtgtcggc gagattgatg tctgcgccct tctcgacgag
     1381 atagagtgcg atgtctttct tgccattatc gagagcttcg agcagtgccg attctccttc
     1441 tttgttcacg gcattgatgt tgcatttggg cagcagcaga tcgatgatgg tcttggcctt
     1501 ctcgaaggtg tagataccgg ctgccatatg cagcggcagg ttgccctcgc gatcggtttg
     1561 atcgagtctc gctccgcgtt cgagaagctt ttgtaccagt tcgactcggc cttccttgac
     1621 cgcggtcatg agtggcgacc gatcatcgta tccgggaagg ttgatgtccg cgccggcctg
     1681 aagcagctgc tcgatcagct cgggtttgtt ggtatcgatg gcccacgtga gcgcgttgtg
     1741 gctttccggc aggcgcagac tcaggtcggc cttgccctga atggccgcag tgatcaaacg
     1801 aatggcctcg tctttatttc ttttaacacc cttgccatca gcaaacatga gtccaagcat
     1861 cagactggcg ttggcctcgt ttttgtcggc cagaggttgt acttctttca aggccgtggc
     1921 aaagtccttc ttgtcgaatg cggcgcgagc ggctgccaga tcggcatacg ccggacacgc
     1981 cagaaccagc gacaaacagc aggcaatcat ggtgcgcatg tgagttcctc cctcggaata
     2041 tgtgttgcgg gtatcatagc ataggtccga gaagttgttg caggcgtgaa ccaatcgtgg
     2101 cagagtagag acaaaatcat cacccggttc ggaggggtcg tcatgagatc gagtctgttt
     2161 tgtgcactct tgggaatctg tgtcatcctg cttgcgggtt gtaccggagg tggcggcggg
     2221 tcagacccgg tcgtgaccac gatcggagtc ggcagtgtcg ccccggagat cgccgcgggc
     2281 aaatggtaca actcagggga ccagccggtc accctgaccg ctcagaagga agcaggcaaa
     2341 cctgtggtgc tgagtttctg ccggtcggat tgccacgtgt gcaaggggca gatgccggcc
     2401 ctggtgggct tgtacaatca gcataaagac aaagccgtgt tcatcacgct ctccgatgaa
     2461 gagaccggac cgatcgagaa gtgcctgcag gattttgcgg cggtgcagtg ggcagtcggt
     2521 gccaagagtg agacctacaa ggcatacgat gtcgagtatg tgccgaccta tgtgctgatc
     2581 gacaagagcg gcaccatccg tcacatgggt cactatgccg tcgatggtgc caccgacgtc
     2641 gacgatctgg aaaccgcgat cgaagaattc gtgaagtgac aaggcgcggt gacgtttccg
     2701 ggtgacagag agtgatgggg cgtacagctc gtgagaaaga aaacggcggt cggaacactc
     2761 cgaccgccgc tttttcgtgg cgtgagatca gaagtcgtgg ctgtagccga ggctccaggt
     2821 gcgggcgcgg aagccgaacg acgtgccgag gttcgaatcg ttcaggctgt cctggtaggt
     2881 ggcgaatact gactgtttct tgtcgaagcg gtgatcgagc cgcatgaacg tctggtggtc
     2941 cttgtcgtgg cggctctgac cggtgaccga acgcacactg gcgttgtcat ggtcgcgatc
     3001 ttcataggtg tagctgccgt agaaggttaa cttgcgattc agatcgcggc gcagaaacag
     3061 gcgcagaccg cgcttgtcgg tatcatagtc ggcaccgtcg gtgtcggcac gggcggcgaa
     3121 gaggccgatt ccgtacgtgc tgctcaggcc tgatctggtg gtctcgcgcc agttctggct
     3181 gatcgaaagg cgcgtgtcgc gaccgtcgcg atcttcgcgg gccagtcgcg gcgcgatctg
     3241 gaagtcgttc cagcggccgc ggacgttcgc gtcgagccag cggccggtcg cctgccgatg
     3301 gacgtacccg acttccatgc cccagttgtg caggtagtca cggtcgccga gttccatgcg
     3361 cccgatgctg ggaacgaatc gccactggtg attcttgcca tgcacgcgcc aggcgagcaa
     3421 cgcattgatc gagtcggttt cgaattgatt cagatggtga tagtcgttgc cggaatagcc
     3481 gacatcgaga tcgatgcgct gatgcttgtc gtcacggaag ttgtagccga ggtcgacgtt
     3541 ccagaacttg ccggtgtcgg cgcggcgcgg cacgaacggc gccagttcgt cggcaatcgc
     3601 cggaatgtta tcgccggctt caacgcccac ctgaatcgac gcggtaaacg gatctttctt
     3661 ctgaggttgt ttcgccagcg gtcgagcggc ctgacgccac tctttatccc agacgcgaat
     3721 ctcttccatg gtgacattca tgcgcttcaa agctgccttt gaatcttcct gaacctggac
     3781 tccggcatac gggcgcatcg aggcgacgat atgcatgcgg gccgcttcgg agcgatttcc
     3841 cagtgcttca tgggcttgtc cgagcaggtt cgcggcttcg tcggccagaa attcgtcctg
     3901 gtaggcctgc ttcaggatg
//
LOCUS       DAORHD010000323         3918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25275_length_3918_cov_4.494176, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000323 DAORHD010000000
VERSION     DAORHD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25275_length_3918_cov_4.494176"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKO06_08070"
     CDS             complement(<1..436)
                     /locus_tag="PKO06_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69636.1"
                     /translation="MNEFMTVIEWWVVPIILCLFLVYVLFLLPPDEAPALVKASSRSG
                     KWAGLVILALFVVSRKESNDTFSFTIPTYPFDYLTTVIATLIGGGFYHAVTQLGTTRA
                     LGFFSFLLSAAVSMTLYSYFYIAAFRSSIVFCTLGVTLGILLQ"
     gene            complement(508..1398)
                     /locus_tag="PKO06_08075"
     CDS             complement(508..1398)
                     /locus_tag="PKO06_08075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69637.1"
                     /translation="MNDAMIRPGAIVSCPLCDRSSGRSLRRCADARLQRCETCGLVYV
                     GARSERPWEVYEQQSASPSAYYTTNALAGRRNASEVLQRICRHVSGGRLLDVGCAGGD
                     FLCVAREAGFAVCGIELNPLSAAHARDHNHLPVLSRRLEEITELSELRDSTSRFGLFS
                     SIHLGDVIEHLPEPRLALLHLRRWLAPGGVLSISTPDFSRPATRLLQIKPQEHLMMFC
                     RTSLTRLLLDCGFSILSLESWDPWRDLAALPHSSTLDVLPVVGRVFRLVLQTLFRRPF
                     PIRLPLRENLLVIARRASPS"
     gene            complement(1438..2199)
                     /gene="cobB"
                     /locus_tag="PKO06_08080"
     CDS             complement(1438..2199)
                     /gene="cobB"
                     /locus_tag="PKO06_08080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HNV69638.1"
                     /translation="MSISFGPRGRSIVVLTGAGISRESGIETFRDGDGLWARHRIEDV
                     ATPEGYERNPQLVQSFYNDRRRQLSTVVPNAAHEALARLERAWTDEVLVVTQNVDDLH
                     DRAGSRKLIHMHGELLKVRCEACRHVYVSSDDVTIDTSCPSCATTGLVRPHIVWFGEM
                     PLEMETIYRTLDQCGMFISIGTSGNVYPAAQFVEIARAARACTVELNLEPSLGASKFA
                     LRRYGPATRVVRAFVDELLATLSVTPKIVPDASRT"
     gene            2289..3797
                     /locus_tag="PKO06_08085"
     CDS             2289..3797
                     /locus_tag="PKO06_08085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HNV69639.1"
                     /translation="MSFFFRCASLFFFLIAVVAGAAEIPPQPFEPVDWGRSYTSDQLS
                     RSDARDGGLPNDPLLDKQYAWRNVGQADDKGQVGIPGCDVRLFDAWRVYKPKQEIILA
                     IVDSGLDLQHEDIDPSCLWVNPGESGLDAQGRDKAGNGVDDDQNGYVDDVHGWNFLKN
                     SPDIQEDHYHGTHVGGQLCARGSNGLGITGGFPGLRIMLVKVFGLGTTLSSEKFAEAI
                     RYAVDNGAKVLSNSYGTPSYTSAMEAAVKYSHDHGTLFVCASGNSRKNMDLEEDKDYP
                     SCYGFENQLVVGATNNRDLSTFSNFGSMVDVAAPGEQMMSTMPKNQYRSFSGTSQACP
                     IAAAAACWVWSMNPQFTALDVKQRLLDSADELPGLRPYVRDGLRLNLYNALLGLPGRR
                     LPAADYSTWATEERVIETSHPYYNNKVVTWDVSVEGAKAFRLHFSRMAIDHFGDTLEI
                     QSRAGQVLAVYNINKDDFWTDAFDGDSVKLVFTANEYVGDYGFTIDRLQVRR"
BASE COUNT          876 a         1095 c         1212 g          735 t
ORIGIN      
        1 gctgcagcag aatgccaaga gtgacgccga gcgtgcagaa cacgatgctg ctgcgaaacg
       61 cggcgatgta aaagtagctg tagagtgtca tcgagacggc cgcggagagc aggaacgaga
      121 agaaaccgag agcacgggtg gtcccgagtt gcgtgaccgc atggtaaaaa ccgcccccga
      181 tcagcgtcgc gatcaccgtg gtcagatagt cgaacgggta ggtgggaatg gtgaatgaaa
      241 aggtgtcatt actctctttg cgactgacga caaacagggc cagaatcacg agtccggccc
      301 acttgcccga gcgggacgaa gctttgacaa gagcaggcgc ttcatccggc ggcagcagaa
      361 acagcacata gaccagaaac agacacagaa tgattggtac gacccaccat tcgatcaccg
      421 tcatgaattc gttcatgcga agctctcctc gtgcaaaagt ccggattcag acagtagctt
      481 agcacgaacc tcggcgcctg tcagaattca ggaaggagac gctcgtcggg cgatgacgag
      541 cagattttcg cgcaagggca agcggatcgg gaatgggcga cgaaagaggg tttgcaaaac
      601 caggcgaaac acgcggccga caacgggaag cacatcgagc gtggaactgt gaggcagggc
      661 cgccagatca cgccaggggt cccaggattc cagcgagaga atggaaaacc cgcaatcgag
      721 aagcagacgg gtgagcgagg tgcggcagaa catcatcaga tgctcttgcg gcttgatctg
      781 caatagacgg gtggcgggcc gcgagaagtc cggcgtgctg atgctcagaa ccccaccggg
      841 agcaagccag cggcgcagat gaagaagagc gaggcgcggt tcgggaagat gttcgatgac
      901 atcaccaaga tgaatggagg aaaacaaccc gaaacgactt gtactatcgc gcagctcaga
      961 aagctcggtg atttcttcga gtcggcggga aagaacgggc aagtggttgt ggtcacgggc
     1021 atgagccgcg ctcagggggt tgagttcgat tccgcagacc gcgaaacccg cttcgcgggc
     1081 aacgcacaga aaatcgcccc cggcgcaacc gacatccaga agtcgcccgc ctgagacatg
     1141 ccggcagatg cgctgcaata cttctgaggc attgcgacgc ccggcgagag cgttcgtcgt
     1201 gtaataggca gagggcgaag cgctctgctg ctcgtagact tcccatggac gctctgaccg
     1261 tgccccgaca tagacgagtc cgcacgtctc gcagcgctgc agacgcgcgt cagcacagcg
     1321 tcgcagactt ctgcccgagg aacggtcaca cagtggacag gacacgatcg ccccgggtct
     1381 gatcattgcg tcattcatga tgcgtccagg ctgtgaattc tcgagtgcct gatcttttca
     1441 agtgcgagaa gcatcaggta cgattttcgg cgtgaccgac aacgtggcca gcagttcgtc
     1501 gacgaatgcc cgaacaaccc gcgtggccgg accatagcgt cgcaacgcaa acttcgaagc
     1561 gccgagcgaa ggctcgagat tgagttcgac ggtgcaggca cgggcggccc gtgcgatctc
     1621 gacgaactgc gcggccgggt acacgtttcc cgaggtgccg atcgagatga acatcccgca
     1681 ctggtcgagg gttcgataga tcgtctccat ctcgaggggc atctctccga accagacgat
     1741 gtggggtcga accagaccgg tggtggcaca cgaggggcat gaggtgtcga tcgtcacatc
     1801 gtccgaactg acgtagacgt gacggcaggc ttcgcaccgc actttcaaca actctccatg
     1861 catgtgaatc agcttgcggc tgccggcccg atcgtgcaga tcatcgacat tctgcgtaac
     1921 cacgagcact tcgtcggtcc aggcccgttc gagtcgagcc agggcttcgt gagcggcatt
     1981 cggcacaacc gttgagagct gccggcggcg atcgttgtaa aacgattgta cgagctgtgg
     2041 gttgcgctca tacccttccg gagtggcgac atcctcaatg cgatgtctgg cccacaggcc
     2101 gtcgccgtcg cgaaaggttt cgattcccga ttcgcgcgaa atgccggccc cggtcaaaac
     2161 gacgatcgat ctgccgcgtg gtccgaatga aatgctcatg cgctgacctc cctgcctgca
     2221 ccgagagaga aacttttccc gctcccggtt ccctgataga atgaagaaaa ttcgtgtcgg
     2281 ggtcaatcat gagcttcttt tttcgttgtg cgagcctgtt tttctttctg atcgccgttg
     2341 tcgcaggtgc tgccgagatt ccgccgcagc cgttcgagcc cgtggactgg ggccgcagtt
     2401 acacgtccga tcagctctcg cgctcagatg cgcgtgatgg gggactgccg aatgatccgc
     2461 tgctcgacaa acagtatgcg tggcgtaatg tcggacaggc agacgacaaa ggccaggtcg
     2521 gcattcccgg gtgcgatgtt cgcctgttcg atgcctggcg cgtgtacaaa ccgaagcagg
     2581 agatcatcct ggccatcgtt gattcagggc ttgatctgca gcatgaagat atcgacccct
     2641 cgtgtctgtg ggtgaacccc ggggaatcgg gacttgacgc tcaggggcgc gacaaggccg
     2701 gcaacggcgt cgacgatgat cagaacggct acgtcgatga cgtgcatggc tggaactttc
     2761 tgaagaactc ccccgacatt caggaagacc actatcacgg cacccatgtc ggcgggcagc
     2821 tctgcgcgcg cggcagcaac ggtcttggta tcaccggagg cttcccgggc ctgcgcatca
     2881 tgctggtcaa ggtgttcggg ctgggtacga ccctgagttc ggaaaagttc gcggaagcga
     2941 ttcgctacgc cgtcgataac ggggcgaagg tgctgtcgaa cagttacggc actccctcgt
     3001 atacctcggc catggaagca gcggtgaaat actcgcacga tcatggcacg ctgttcgtct
     3061 gcgcctcagg gaactcgcgc aagaacatgg atctcgagga agacaaggat tacccgtcgt
     3121 gttacgggtt cgaaaaccag ctggtggtcg gggccaccaa caaccgcgat ctctcgacct
     3181 tcagcaactt cgggtcgatg gtcgatgtcg ccgctccggg cgagcagatg atgtcgacga
     3241 tgcccaagaa ccagtaccga tccttctcgg gcacctcaca ggcgtgcccg atcgcggccg
     3301 cggccgcctg ctgggtctgg tcgatgaacc cgcagtttac ggcacttgac gtgaaacagc
     3361 gcctgctcga ttcagccgat gaactgcccg gattgcgccc atacgtcaga gatggtctgc
     3421 gcctgaactt gtacaatgcg cttctggggt tgcctggtcg acggctgccg gcagcggatt
     3481 attcgacctg ggcgacggaa gaacgtgtca tcgaaacatc gcatccctac tataacaaca
     3541 aagttgtgac ctgggacgtt tccgtggaag gagcaaaggc gtttcgcctg catttttccc
     3601 gcatggcgat cgaccatttc ggagacacgc tcgagatcca gagtcgtgcc ggacaggtgc
     3661 tggccgtcta taacatcaac aaggacgact tctggacgga tgctttcgac ggtgattctg
     3721 tgaagctcgt ctttaccgcc aacgaatacg tcggcgacta tggattcacg atcgatcgtc
     3781 tgcaggtgcg acgctgagct tcaacgatcg ctggccggag gatagaagcg ggcgccgtgt
     3841 agctgctgca gtggagcatg tagaggctgc agttccttct cgatgacgaa gacgatgaaa
     3901 tgacccggca gtttcatg
//
LOCUS       DAORHD010000324         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25376_length_3907_cov_3.584891, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000324 DAORHD010000000
VERSION     DAORHD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25376_length_3907_cov_3.584891"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKO06_08090"
     CDS             <1..183
                     /locus_tag="PKO06_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNV69640.1"
                     /translation="PGCGGCASGQLGMTGKDEIQISTSNRNFKGKQGDGETYLASPLV
                     AAASAVAGVITDPRDL"
     gene            203..754
                     /locus_tag="PKO06_08095"
     CDS             203..754
                     /locus_tag="PKO06_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HNV69641.1"
                     /translation="MSKPTTLKGKVWVIADAQGKLIEDIDTDMIFHNAHLAITDIAKM
                     GPHAFGNLPGWEDFPKKAKPGDMLILGKNFGAGSSRQQAVDCFTALGTVALIAESFGA
                     IYKRNAINSGMPLLTKEGLLGDPAKPNFQNGDEIEIDLAKATLKNLTRKIEMKLDAFS
                     KVQMDIYQAGGLYAYGKTLEAGK"
     gene            900..2486
                     /locus_tag="PKO06_08100"
     CDS             900..2486
                     /locus_tag="PKO06_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69642.1"
                     /translation="MQRKLLLTSVFKPFGVDDAFGRKENVFELMKNQVTRAQEAFSIR
                     SHNRSFGLSLMALNIETPTTVLDFPTQDEFMQELRRGHYSHVGISFIVPSFEKAAHMA
                     KLVRLHSPWSKIILGGHGVSIAETERLIEHDEICRGDGVTWLRSYFGERVGAPIEHPI
                     MRLAFNSRLFGLPIPNAKAILIPAVGCPGRCDFCATSHFFQGATEYFPDPESLFATMC
                     HISDTLKTHEFFVLDEIFLKNADRARALAALMRRHGRRFQLDIFSSLESLAALPLQTL
                     IELGINFVWIGIESKDDLYAKTRGLDARRIIGDLRRHGISPLTSGILFMDHHTPDNID
                     AEIDHLISLQPDFIQFMELAPYPGTALYKKLEAQKRIRSEVPYQEWHGQDQIWFRHPH
                     FTPQRSKEILDEAFEREYQALGPSLLRMAETRLLGVENGVFCPGDAFLEARYRDLETI
                     AKRLMPLLPVCERLAPNERVRARARVLIERYQRRFGPPSLLQSALSLTAAFCARREKR
                     HLRLNLPPLQPQTFIERFRH"
     gene            2690..>3907
                     /locus_tag="PKO06_08105"
     CDS             2690..>3907
                     /locus_tag="PKO06_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV69643.1"
                     /translation="MMYRSRFVVLAVALLVLVGMQAAFAAEVAGKFIVRFVPDPSAPQ
                     KGSSHKTIVSHLQQQLERNLADQGKLGGAKILNKLWIINGVEVAASPAQVATLKKNPR
                     IASVTPVQYRKYIDFGKPVEVKAEPGQVQWSIAKVRAPEVWKEFNIDGSGVIVGHLDT
                     GVAANHPSLKGKVLAFRDFTPANKPESYDDQGHGTHTAGSIAASDGIGVAPGARLIVA
                     KVFDRQGSAEDAWLLGAMQWVMDPDGNAETNDGPALVSNSWGSDTTDDRTFWDIVKVW
                     EAADIVPVFAAGNNGPSGKCGTPANFPHSWAVAATTNKDGIAYFSSVGPSVWDGVTLT
                     KPDIAAPGYGVISCSTSGGLVSNMGTSMACPHVAGLAALMLQANPKLTIAEVRQIAEA
                     TAVDLALPARITSS"
BASE COUNT          789 a         1243 c         1186 g          689 t
ORIGIN      
        1 cccggctgcg gcggttgcgc gtctggacag ctcggcatga ccggcaaaga cgagattcag
       61 atcagcacca gcaaccgcaa cttcaagggc aagcagggcg atggcgaaac ctatctggcc
      121 agccccctgg tggcggcggc ttcggcggtc gcgggcgtca tcaccgaccc gcgcgatctg
      181 tgagcagaaa ggatacacga cgatgagcaa accaacgaca ctcaaaggca aagtctgggt
      241 catcgccgat gcccagggca aattgatcga agatatcgac accgacatga tcttccataa
      301 cgcgcatctg gcgattaccg acatcgccaa gatgggcccg cacgccttcg gcaacctgcc
      361 cggttgggag gactttccga agaaggcgaa gccgggcgac atgctgattc tcggcaagaa
      421 cttcggcgcg ggcagttcgc gtcagcaggc cgtcgactgc ttcaccgcgc tcggcacggt
      481 cgctctgatc gccgagtcgt tcggcgcgat ctacaaacgc aacgccatca actcgggaat
      541 gcccctgctg accaaagagg gcctgctcgg cgacccggcg aagccgaact tccagaacgg
      601 cgatgagatc gagatcgatc tggccaaagc cacgctgaag aacctgaccc gcaagatcga
      661 gatgaaactc gatgccttca gcaaggtcca gatggacatc taccaggccg gcggcctgta
      721 cgcgtacggc aagaccctcg aagccggaaa atagacaccc cgcggacgca gcgaagcccc
      781 ctccccgcga gggggctttc tgttatcatc gatgcagcgc cgattcattt cggctctggg
      841 cagtgcgaga gagtctgcac cccgccgttc actcagtcga aaaggagtcc gtgtcggaca
      901 tgcaacgaaa actgctgttg acctcggtgt tcaagccgtt tggggtcgat gacgcattcg
      961 ggcgcaagga gaacgtcttc gagctgatga agaaccaggt gacccgcgcg caggaggcgt
     1021 tctcgattcg gtcgcacaac cgcagcttcg ggctatcgct gatggccttg aacatcgaga
     1081 cgccgacgac ggtgctcgat tttccgaccc aggacgaatt catgcaggaa ctgcggcgcg
     1141 gccactacag ccatgtcggc atctcgttca tcgtgcccag tttcgagaaa gccgcccaca
     1201 tggcaaagct ggtgcgcctg cacagcccat ggtcgaagat catcctcggc gggcacggcg
     1261 tctcgatcgc cgaaaccgag cgcctgatcg agcatgacga gatctgccgc ggcgacggcg
     1321 tgacctggct gcgctcctat ttcggcgaac gggtcggcgc gcccatcgaa cacccgatca
     1381 tgaggctggc gttcaattcg cgcctgttcg ggctgccgat cccgaacgcg aaggcgatcc
     1441 tgattccggc cgtcggatgc ccgggccgct gcgatttctg cgccaccagc cacttctttc
     1501 agggcgcgac cgagtatttc cccgacccgg aaagtctgtt cgcgaccatg tgccacatct
     1561 cggacaccct caagacccac gagtttttcg tgctcgacga gatctttctc aagaatgccg
     1621 accgggccag agctctggca gcgctcatgc gccggcacgg gcggcggttt cagctcgaca
     1681 tcttctcgtc gctcgaatcg ctggcggccc tgccgctgca gacactgatc gaactcggca
     1741 tcaacttcgt ctggatcggc atcgaaagca aagacgacct gtacgcaaaa acccgcggtc
     1801 tcgatgcacg gcggatcatc ggtgacctgc gtcgccacgg catctcgccg ctgacctcgg
     1861 gaatcctgtt catggaccat cacacccccg acaacatcga cgccgagatc gaccacctga
     1921 tctcgctgca gcctgatttc attcagttca tggaactggc cccctacccc ggcacggcgc
     1981 tgtacaagaa gctcgaagct cagaaacgca tcagaagcga ggtgccgtat caggaatggc
     2041 acggtcagga tcagatctgg ttccggcacc cgcacttcac gccacaacgg tcgaaggaga
     2101 ttctggacga agcgttcgag cgcgagtatc aggccctcgg cccgagcctg ctgcgcatgg
     2161 ccgagacgcg tctgctcggg gtcgaaaacg gcgtgttttg tccgggtgac gcatttctgg
     2221 aagcacggta ccgtgatctc gagacgatcg cgaagcgact gatgcctctg ctgccggtct
     2281 gcgagcgcct ggctcccaac gagcgcgtgc gcgcccgcgc tcgtgtcctg atcgagagat
     2341 atcagcggcg cttcggccca ccatcgctgc tccagtcggc cctgtcgctg accgccgcat
     2401 tctgcgcgcg ccgcgaaaaa cggcatctgc gcctgaacct gccgccgctt cagccgcaga
     2461 cattcatcga gcggtttcgt cactgacgaa cccgaggcgt gcccccgcgg aatcaggccg
     2521 cgcggcttgt cgatcgcgca ccaggggcgg gcggacaaat cggagtcagg atgccggctc
     2581 cttaaccgta gaggtccccc tttcccgtat caggagtggg ttgcggtacc gggtatgctg
     2641 tgctacgctg gaatgcttcg aaacaaattc gattcaggga gtagggataa tgatgtatcg
     2701 ttcccggttc gttgtcctcg ccgttgctct tctggtgctg gtcggtatgc aggcagcgtt
     2761 tgccgccgag gtcgccggca agttcatcgt tcgtttcgtg cctgacccct ctgcgccgca
     2821 gaaaggcagt tcgcacaaga cgatcgtcag tcatctgcag cagcagctcg agcgcaacct
     2881 ggccgatcag ggcaaactcg gcggagcgaa gatcctgaac aagctctgga tcatcaacgg
     2941 cgtcgaagtg gccgcgagcc ccgctcaggt cgcgacgttg aaaaagaacc cgcgcatcgc
     3001 gagcgtgact ccggtgcaat atcgcaagta catcgatttc ggcaagccgg tcgaagtcaa
     3061 agccgagccg ggtcaggtgc agtggagcat cgcgaaggtt cgcgcccctg aagtctggaa
     3121 ggaattcaac atcgacggca gcggcgtcat cgtcggccat ctcgacaccg gtgtggccgc
     3181 caatcatcca tcgctcaagg gcaaggtcct ggcgttccgc gattttacac cggcgaacaa
     3241 gcccgaatcg tatgacgacc aggggcacgg cacgcacacc gccggttcga tcgccgcttc
     3301 tgacggcatc ggcgtggctc cgggcgcgcg cttgatcgtc gccaaagtgt tcgacaggca
     3361 gggcagcgcg gaagacgcct ggttgctggg tgccatgcag tgggtcatgg atccggacgg
     3421 caacgccgaa accaacgacg gcccggctct ggtgagcaat tcatggggtt ccgacaccac
     3481 cgacgaccgc accttctggg atatcgtcaa agtctgggaa gccgcggaca tcgttccggt
     3541 cttcgccgcc ggcaacaacg gcccgtcggg taagtgcggc acgcccgcga acttcccgca
     3601 cagctgggcg gttgcggcca caacgaacaa ggatgggatc gcatatttca gcagcgtcgg
     3661 cccgagcgtg tgggacggcg tcacgctgac caagcccgac attgccgctc ccggctacgg
     3721 ggtcatcagc tgttccacca gcggtggtct cgtgagcaac atgggcacct cgatggcctg
     3781 cccgcacgtc gctggtctgg cagctctgat gctgcaggcg aatccgaagc tgacgatcgc
     3841 tgaagtccgg cagatcgccg aagcgaccgc ggtcgatctg gcactcccgg caaggataac
     3901 aagttcg
//
LOCUS       DAORHD010000325         3903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25411_length_3903_cov_3.329262, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000325 DAORHD010000000
VERSION     DAORHD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25411_length_3903_cov_3.329262"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKO06_08110"
     CDS             <1..286
                     /locus_tag="PKO06_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNV69644.1"
                     /translation="CLWSPTIAVRWAAQRTIGLCFLIELSQLCQADWLNAIRQTRLGA
                     IVLGFGFLWSDLACYICGAGVAALIDRGCLVSESNFSSVTTSTPDIMEWK"
     gene            312..3155
                     /locus_tag="PKO06_08115"
     CDS             312..3155
                     /locus_tag="PKO06_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69645.1"
                     /translation="MRHYLLMVLVVAGLFCVWCDSAGAQVEAPAVPTIEWKATSEGWK
                     QAVIGDGSEPTVIEGTLSPRQDGLFYRSGEEKPYTGWMYSSNPNHPPETLTVIWHYLN
                     GTRHGWEFRFNKSNDGKQGSIHSICEYKNGKPDGVVLRYHENGKLASQEGNLDGRQHG
                     PAKSWFENGQLWQERAYKNGERDGLEKCWFQNGKQNSEQSYTDGKLNGPSTMWYENGN
                     MRETGEYTLVDKNGEKRSEKHGLWKEFREEDGTLSAEKVYDKGEMNRDTWYNKSGVKT
                     KETMYAPGRHALIVTFDEASGFPASLYMQKDHTSHGTSIRWYVDKKQISAVENHFEGK
                     LHGPFEYFGFKGERTRNGYHRDNTESATPVERTRVPELEDLLRQYLRMGTPKGANWPE
                     LQTSAPKAEPPATVTPEPVAPLQTPPSPPDDQRKQVAVATETPPSPVATTPQASESSS
                     LTSMFDAVVSGIAGPAGDGLLRSAHVEPPATVEPSPGARVNAVPDRPAAAGAPVVPEV
                     KSPARDAGGSAAALVTCPSCGKKVEAGAFCDECGSSLTSEKSCVKCGKTLAAKSKFCK
                     SCGAPQSPTKEPARPTTSTSAPVAPTEPVSIEKLLQRARESEKNEKFAELLNRAHELL
                     QRDPGNAEGLYLRGIALRKTGKPEKAVADLQRVTELQPGNALAHYQLGHAWRAQKNLQ
                     KAIVSFEKTARLDPRHWQSRYNLFDCLSEMKRFNESLVYLQEAEEIEPHNELIQHSLG
                     FNLVQLERLREALEKFLPLAKNTKDFPAIQKNIGACYYGLNEPKKALPYFQAHLRMNP
                     EDQWIKERIAEISGGASGSGNSVKGDLSSYINHPTLQRVDLKMQEIYKDLQAMGSQNN
                     AQVKVYLDRITPFLREAKKSMSQLQPSTNEVRRAHALYIESIGDLADGAKQVSQAIRK
                     DSRALFDSGLAVIKRASEKRAKMLELFRTLGQQ"
     gene            3247..3873
                     /locus_tag="PKO06_08120"
     CDS             3247..3873
                     /locus_tag="PKO06_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69646.1"
                     /translation="MLTPMKMKRYWMLMMVIVGLVVWHAGPSALFASGSGEEAAVVVS
                     DSEPPASIVDAIAPILMDRRSQQAIANLKHRSFTDMTVWSDLHGYISLKSVKNADTKS
                     LQLVRDLEKHGIGKNEGAASFMAILIPQISELHQAMKRVNPATEKVGLAHTAFVQSLE
                     WFAEGARLVELGSRKESVKIRENGWAAVRRALEKRREALLMFRQMGGR"
BASE COUNT          942 a          997 c         1197 g          767 t
ORIGIN      
        1 ctgcctgtgg tcaccgacca tcgcggttcg atgggccgca caacggacga tcggtctctg
       61 ttttctgatt gaactcagtc aattgtgcca ggccgactgg ctgaacgcga ttcgccagac
      121 ccgactgggc gccatcgttc tcggcttcgg ttttctctgg agcgatctgg catgttacat
      181 ctgcggtgct ggggtagccg ctctgatcga tcgcgggtgt ttggtcagcg agtcgaattt
      241 cagcagtgtc acgacatcaa ctcctgatat catggagtgg aagtgagagt agtcgcctag
      301 ggaggttcca tatgcgtcat tatctgttga tggtgcttgt tgtggctggc ctgttctgcg
      361 tttggtgcga ctccgccgga gcgcaggttg aagcgccggc ggtgccgaca atcgagtgga
      421 aggccacgtc cgagggctgg aagcaggcgg tgatcggtga tggatctgag ccgaccgtca
      481 tcgaaggaac gttgtcgccg cgacaggatg gcctcttcta ccgttccggt gaagagaaac
      541 cgtataccgg ttggatgtat agctccaatc cgaatcatcc gcctgaaacc ctgacggtca
      601 tctggcatta tctgaacgga accaggcacg gctgggaatt tcgtttcaac aagtcgaatg
      661 acggcaaaca gggctcgatt cacagcatct gcgaatacaa gaacggcaaa cctgacggtg
      721 tcgttcttcg ctatcacgag aatggaaagc tcgcttcaca agaaggaaat ctggacggtc
      781 gacaacatgg accagcgaaa tcctggttcg aaaatggaca actctggcag gaacgagcgt
      841 ataagaatgg tgaacgcgat ggcctcgaga agtgctggtt ccagaacggg aaacagaaca
      901 gcgagcagtc ttacactgat ggcaagctga acggaccttc gacgatgtgg tacgaaaacg
      961 gcaatatgag agaaaccggt gaatacacgc tcgtcgataa gaacggtgaa aaacggtctg
     1021 aaaagcatgg tctctggaag gagttccgcg aggaagacgg aacgctgtca gcggagaagg
     1081 tctacgataa aggagagatg aatcgggata cctggtacaa caagtcgggt gtgaagacaa
     1141 aggaaaccat gtatgcaccc ggaagacatg ccctcatcgt cacgtttgac gaagcaagtg
     1201 ggtttcccgc atctctctac atgcagaaag accacaccag tcatgggacg tcgatcaggt
     1261 ggtatgtgga caaaaagcag atcagcgccg ttgaaaacca tttcgaaggc aagctgcacg
     1321 gaccttttga atacttcggt ttcaagggcg aacggacgag gaacggctat catcgtgaca
     1381 ataccgagtc ggctacgccg gtcgaacgta ctcgtgtgcc agaactcgaa gaccttctgc
     1441 gccagtatct gcgcatgggc actccgaagg gcgcgaactg gcctgagcta cagaccagcg
     1501 caccgaaagc cgagccgccc gcgacggtga cccctgaacc ggttgcgccg ctgcaaacac
     1561 cgccgtcgcc accagatgat cagcgaaagc aggtcgccgt ggcgactgag accccgccat
     1621 cccccgtcgc cactacgccg caggcttccg agtcgtcttc actgaccagt atgttcgacg
     1681 cagtggtgag tggtattgct ggaccggctg gagacggtct tctcagatcg gcgcacgtgg
     1741 agccgccggc cacggtggaa ccgtcgcccg gggccagagt gaatgctgtt cccgataggc
     1801 ctgcagctgc gggagcaccg gttgtgcccg aggtgaagag ccccgcgcga gatgctggtg
     1861 gttctgctgc tgctctcgtg acctgcccgt cgtgcggcaa gaaggtcgaa gccggggcat
     1921 tctgcgacga gtgtgggtcc agtctgactt ctgagaaatc ctgcgtcaaa tgcggaaaaa
     1981 cactggcagc gaaatcgaaa ttctgcaaga gttgcggtgc gccgcagagc cccacgaagg
     2041 agcctgccag accgacaacc tccacatccg ctcccgtggc gccgaccgag cctgtgtcga
     2101 tcgagaagct gctgcagagg gcgcgagaga gtgaaaagaa tgagaaattc gctgaactgc
     2161 tgaatcgagc tcatgagctt ctccagcggg atcctggaaa cgcggaaggg ttgtatctga
     2221 gaggtatcgc tttacggaag accggaaagc cggaaaaagc ggttgctgac ctgcagcggg
     2281 tcaccgagct acagcccgga aacgctctgg cgcattatca gctgggacat gcctggcgag
     2341 ctcagaaaaa cctgcagaag gcgattgtga gtttcgaaaa gacggctcgg ctcgatccca
     2401 gacactggca gagccggtac aatctcttcg actgtctgtc cgagatgaaa cggttcaacg
     2461 agtcactggt gtatctgcag gaagccgaag agatcgaacc gcacaacgag ttgattcagc
     2521 acagcctcgg cttcaatctc gttcaactcg agcggcttcg tgaagcactc gaaaaatttc
     2581 tgccgctcgc gaagaatacg aaggattttc cggccattca gaagaacatt ggcgcgtgct
     2641 actacggact gaatgaaccg aagaaagccc ttccctactt ccaggcacat ctgcggatga
     2701 acccggaaga tcagtggatc aaggaaagga ttgctgagat ctctggtggt gcaagtggaa
     2761 gcgggaacag cgtaaaggga gatctgagct cgtacatcaa tcatccgacg ctgcagcgtg
     2821 tcgatctgaa gatgcaggaa atctataagg acctgcaggc catgggcagt cagaacaatg
     2881 cccaggtgaa agtctatctc gatcggatca ctccgtttct ccgggaggcg aagaagtcga
     2941 tgtcccagtt gcagccgtcg accaacgagg tgcggcgtgc gcatgccctg tacatcgagt
     3001 cgattggcga cctggcagac ggggccaaac aggtgagtca ggcgattcgc aaggattcgc
     3061 gtgcgttgtt cgattcaggg ctcgcggtga tcaagcgtgc cagcgaaaaa cgcgcaaaaa
     3121 tgctcgagct gttccgcact ttgggccagc agtaatcgag ctgtagggtc aatctcaggt
     3181 ataatgagag acgggagaaa cctattctgt ctcctggttc atcaagacga caaggggggg
     3241 agcgcaatgc tgacgccgat gaagatgaag agatactgga tgctgatgat ggtcatcgtg
     3301 ggtttggttg tgtggcatgc cgggccatct gccctgttcg cgagtggttc aggggaagaa
     3361 gcagcggtgg tcgtgtccga ttctgaaccg ccggcgtcga ttgtcgatgc cattgccccg
     3421 attctgatgg acaggaggag tcagcaggcg atcgccaacc tgaagcacag gtctttcacc
     3481 gacatgaccg tgtggtcgga cctgcatgga tacatttccc tgaagtccgt gaagaacgcc
     3541 gacacgaaat cgctgcagct cgtacgagat ctcgaaaagc atggcatcgg gaagaatgag
     3601 ggtgccgctt cattcatggc catactgatt cctcagatca gtgaactgca tcaggccatg
     3661 aagcgcgtca atcccgcgac agagaaggtt ggtctggctc acacggcttt cgtccaatca
     3721 ctggaatggt tcgcggaagg ggctcgactc gtggaactgg ggagccgcaa agagtcggtg
     3781 aagattcgag agaacgggtg ggctgcggtg cgccgcgctt tggaaaaacg ccgcgaggca
     3841 ctgctcatgt ttcggcagat gggtgggcgc tgaaacttgt gaatcagacg ataacgtcag
     3901 ata
//
LOCUS       DAORHD010000326         3902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25421_length_3902_cov_3.665454, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000326 DAORHD010000000
VERSION     DAORHD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25421_length_3902_cov_3.665454"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKO06_08125"
     CDS             <1..694
                     /locus_tag="PKO06_08125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV69647.1"
                     /translation="IRFRGIAVGEVEQVVLAPNLKGVLVQARLRPDALGLARDGTTFW
                     IVRPQASLYGIQGLETLIGANYIGVLPGTGAPKFVFAGSEEQLAGDHAGDLFEIVLEA
                     PTCANLRPGMPIKYKKFNIGRLLAIRMKHDLTGFRIRAAIEPAYQSLIRERTVFWNSS
                     GLNFSFTLRGGIQLDVDSLQSILAGGLTIGIPDDPGQPVPVGHLFSLLEMPPSGWESW
                     NPPITLTAAGTP"
     gene            806..1711
                     /locus_tag="PKO06_08130"
     CDS             806..1711
                     /locus_tag="PKO06_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015180626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HNV69648.1"
                     /translation="MVEHSGKGNPFGGNWTDAKLRVLAKYLQAFTTALSRQSFITGYI
                     DAFAGTGYREPVRDRDDADPAQGVLFPDLATPDTQTLLDGSARIALQVSPRFSRYIFI
                     ERDSQRCSHLNALKSEFPALADDIRIFQGDANTHIQAICQTSWKNHRAVLFLDPYGMQ
                     VEWQTIEAIAGTKAIDLWLLFPLGMGVNRLLPRSGNIPDGWRRRLDLLLGTSDWYDEF
                     YRVEASPDLFNSEQAHVIKATMDTIARYFVKRLKSIFPEVTEEPGILKNSSNNPLYLF
                     CFAAGNQKGGKIGLKIANDILKGIR"
     gene            1711..2445
                     /locus_tag="PKO06_08135"
     CDS             1711..2445
                     /locus_tag="PKO06_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HNV69649.1"
                     /translation="MALGSSIEWTETTWNPVTGCTKVSPGCRHCYAERMTARLQSMGQ
                     ANYARGFEVATHEHMLTAPLRWKKPQTIFVNSMSDLFHVDVPESFIKKVFDVMNQAHW
                     HTFQVLTKRSERMADLGSRLSWPGNVWAGVSVESAEYLYRIDHVRKVPAQVRFVSFEP
                     LLAGLGEIDLRNIHWAIVGGESGPGARPIEESWVAEIRDQCLHAGVPFFFKQWGGVNK
                     KKAGRLLHGRIWDSRPQPHTAASVAL"
     gene            2597..>3902
                     /locus_tag="PKO06_08140"
     CDS             2597..>3902
                     /locus_tag="PKO06_08140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNV69650.1"
                     /translation="MITGEIRSQIDRVWDAFWSGGISNPLEVIEQITYLLFLRRLDDL
                     HTLEEVKASRFKKPMERRIFPEGKDERGRGFEDLRWSRFKHFVAPEMFIVLSEHAFPF
                     LRKIGGDDSTYAHHMKDARFTIPTPALLAKVVDLLDNVPMDERDTKGDLYEYMLDKIS
                     QSGQNGQFRTPRHIIRLMVELTAPTPADIVCDPACGTAGFLVAVGEYLREKHSKALHD
                     QKQKEHFHHRLFHGFDFDNTMLRIGSMNMLLHGVENPDIRYRDSLAQEHSGDEETYTL
                     VLANPPFAGSLDYESCAKDLQKIVKTKKTELLFLALFLRLLKPGGRAAVIVPDGVLFG
                     SSKAHKELRKILVEDQKLDGVVSLPGGVFKPYAGVSTAILLFTKTNSGGTDHVWFYDV
                     EADGWSLDDKRTALLAEDKLGPTPKTTLTDEEQLDLIRFTVEK"
BASE COUNT          865 a         1151 c         1068 g          818 t
ORIGIN      
        1 catccgcttt cgcggcatcg ccgtcggcga agtggaacag gtcgtcctgg ccccgaacct
       61 gaagggcgtt ctcgtgcagg cccgcctgcg ccctgacgcc ttgggcctgg cccgcgacgg
      121 caccacgttc tggatcgtac gaccgcaggc cagcctgtac ggaattcaag ggctcgaaac
      181 cctgatcggc gccaactata ttggagtgtt acccggtact ggcgcaccga agttcgtctt
      241 cgccggtagc gaagagcagc tcgcgggcga tcatgccggc gacctgttcg agatcgtgct
      301 cgaagcgcca acgtgcgcca atctccgccc cggcatgccc atcaagtaca aaaagttcaa
      361 tatcggccgt ctgctcgcga tccgcatgaa gcacgacctc accggattcc gcatccgcgc
      421 cgccatcgag ccggcatacc agtcgttgat tcgcgagcgc accgtcttct ggaactcgag
      481 cggtctcaac ttttcgttca cgctccgcgg tggcatccag ctcgacgtcg actccctgca
      541 gtccattctc gccggcgggt tgaccatcgg cattcccgat gaccccggcc aaccagtacc
      601 agtcggtcat ctcttttccc tgctcgaaat gcctccttcc ggctgggaat cctggaaccc
      661 gcccatcact ctcactgcgg caggcacccc ctgaaaactt ggcacctgca cccgagagaa
      721 ggggaacagg aactcccatt caaaagaaga cttcgcattc agttcagcac tttccaaaca
      781 actgcggatt gaatatagtg tctccatggt tgaacactct ggcaaaggaa atccattcgg
      841 cggaaactgg acagacgcga aacttcgagt ccttgccaaa tatctgcagg ctttcacaac
      901 ggccctttcg cggcagtcgt tcatcaccgg ttacatcgac gccttcgccg gcaccggtta
      961 tcgagagccg gtgcgtgatc gggacgacgc agacccagcc caaggcgtgt tgtttcctga
     1021 ccttgccaca ccggacacgc aaaccctgct ggacggttcg gctcgcatcg ccttgcaggt
     1081 cagtcctcgc ttcagtcgct acatcttcat tgagcgcgat tcccagcgtt gttctcacct
     1141 gaacgctctc aagagtgagt ttcccgctct cgctgacgac atccggattt tccagggcga
     1201 cgctaacacg catattcagg ccatctgcca gacaagctgg aagaatcacc gggccgtgct
     1261 ttttctcgac ccttatggaa tgcaggtcga atggcagacg atcgaagcaa tagccggtac
     1321 aaaagccatc gatctctggc tattgttccc cttagggatg ggcgtaaatc gtctgttgcc
     1381 acgatcgggc aacattccag acggctggcg gaggcgtctc gaccttctcc ttggcacatc
     1441 agactggtac gatgagttct accgcgttga ggcaagccct gacttgttca acagtgaaca
     1501 ggctcacgtg atcaaagcaa ccatggacac gatcgccagg tattttgtaa aacgcctgaa
     1561 gtccatcttt ccggaagtta ccgaggaacc cggaattctg aagaattcgt ccaataaccc
     1621 actgtatttg ttctgctttg ccgccggaaa ccaaaaaggc ggaaagatcg gcttgaaaat
     1681 cgcaaacgat atcctcaaag gaatacgctg atggcactcg gctcaagtat cgaatggaca
     1741 gaaaccacct ggaacccggt cacgggctgt acgaaagtca gccctggctg tcgccactgt
     1801 tacgccgagc gcatgaccgc tcgtctccag tctatggggc aggcgaacta tgcgcgaggg
     1861 ttcgaggtgg cgacgcacga gcacatgctg acagcgcccc tgcgctggaa gaagccacaa
     1921 accatctttg tgaattccat gagcgacctg tttcacgttg atgtacccga atcctttata
     1981 aaaaaagtct tcgacgtcat gaaccaggca cactggcata cctttcaagt gctgaccaaa
     2041 cgttctgaac gcatggctga tctaggaagc cgactctcct ggcccggcaa cgtgtgggcc
     2101 ggcgtgagcg tcgagtcagc cgagtatttg taccgcatcg atcatgtgcg gaaagttccg
     2161 gctcaggtgc gctttgtctc atttgaaccc ctgcttgccg gtcttggcga gatcgatctg
     2221 cgcaacattc attgggcgat cgttggcggt gaatcaggcc ctggcgccag accgatcgaa
     2281 gaatcctggg tcgcggaaat ccgtgatcaa tgcctgcatg caggcgtgcc cttctttttc
     2341 aaacagtggg gcggagtgaa caagaaaaaa gcaggcaggc tgctgcacgg ccgaatttgg
     2401 gactcacgac ctcagccgca cacagccgca agtgtcgctc tctgatcaat cgatcgatgg
     2461 gggcggacgg agacgaagct gtgggaactg ccagttctca ttcttgttgg ctcactgaga
     2521 gaaccgtgat atgatgacga agtcctgaag cgggtcttcg gggcaccagc aaactcggca
     2581 gacaagtgag gacgtcatga tcaccggtga gattcgcagt cagatcgacc gcgtctggga
     2641 tgccttctgg tccggaggca tctcgaaccc gctcgaagtc atcgagcaga tcacctatct
     2701 tctttttctg cgacgtctcg acgaccttca cacgctggaa gaagtgaagg cgagccggtt
     2761 caagaagccg atggagcgcc gcatctttcc cgagggaaaa gacgaacgag gtcgggggtt
     2821 cgaggacctg cgctggtcgc ggttcaagca ttttgtcgcg cccgagatgt tcatcgttct
     2881 cagcgaacat gcgttcccct ttctgcgcaa aatcggcggc gacgactcga cgtatgcgca
     2941 tcacatgaag gatgcccgct tcaccattcc aacccccgct ctgctggcca aggtcgtcga
     3001 ccttctcgac aacgtgccga tggatgaacg agacacgaag ggcgatttgt acgagtacat
     3061 gctcgacaaa atcagccagt caggccagaa tggacagttt cgcaccccgc gccatatcat
     3121 tcgtctgatg gtcgagctga ccgcgccgac cccagctgac atcgtctgtg acccagcctg
     3181 tggtaccgcg ggctttctgg tcgcagttgg cgagtatctg cgtgagaaac attcaaaggc
     3241 tctgcatgac cagaagcaga aagagcactt tcatcatcgg ctgtttcacg gcttcgattt
     3301 tgacaatacg atgctgcgca tcggcagtat gaacatgctg ttgcacgggg tcgagaaccc
     3361 ggacattcgt tatcgcgact cgcttgccca agagcactcg ggggacgagg aaacgtacac
     3421 gttggttctg gcgaatccgc cctttgccgg ctcacttgat tacgagagtt gcgccaaaga
     3481 tctgcagaag atcgtcaaga cgaagaagac cgaactgctg tttctcgcct tgtttctgcg
     3541 gctgttgaag ccgggcggcc gggcggcggt catcgtgcct gacggtgtgc tgttcggctc
     3601 atcgaaagcc cacaaagaac tgcgcaagat cctcgtcgag gaccagaagc tcgacggcgt
     3661 cgtctctctg ccgggcggcg tgtttaagcc gtatgccggc gtctcgacgg cgattctgct
     3721 gttcacaaaa acaaactcag gcggcaccga tcacgtctgg ttttacgatg tcgaagccga
     3781 tggctggtcg ctcgatgaca aacgcacagc gcttttggcc gaagacaaac tcggaccaac
     3841 accgaaaacc acgctgacgg atgaggaaca gctggatctg atccgcttca ctgtcgaaaa
     3901 ag
//
LOCUS       DAORHD010000327         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25438_length_3901_cov_3.145346, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000327 DAORHD010000000
VERSION     DAORHD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25438_length_3901_cov_3.145346"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKO06_08145"
     CDS             <1..687
                     /locus_tag="PKO06_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69651.1"
                     /translation="MSGGGLGNLAGAFDELMFLLPDFYQGGRVVVEPMMDFTATLGTL
                     VQIEDSGCRFEGIDQQMIEDSGWVGFRYPWSEQPVADRLRELSLRIGQEAHQMGARGY
                     LNLDWGILDGGGELKIHALESNFRHNGLSHLLDLRRRYFGEAGHSLHIILRETFRLPG
                     SELTWEQLVQRLESVRHEDEPLLIAGPGREFGVIPVLPPANHTCGIAVFGRSQAEAET
                     LLQKVYRALA"
     gene            complement(758..1807)
                     /locus_tag="PKO06_08150"
     CDS             complement(758..1807)
                     /locus_tag="PKO06_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69652.1"
                     /translation="MVAFFRSLSLTVLVLAFGASLASAETSLCGEQATIPAPLEHLRL
                     GGFARALQQAERARAAGDRRVEDQALISALDSLNQLEQHPANAVLQHDFANLLERHRF
                     SAAHRLGSTPINQLWCPGSRAAKGGLYEHLDEAIRSHERRLPQYARLTGGLSTPLFRK
                     IARLQRLNLPIAWDIDCRARRFQKQGIPIICGDLVSMNTIAPLDRAPIHHRSLSAGEA
                     ERLRFSLRSLQKQGLKLVSANRFRDLAQMTFDLLQTVVRLENQADCHLAMTRHMLESL
                     GLTALHADRYLRQSADTAELSRLFLTIQLFPMQECLVTDRKAQACHALGVGVIYNDVP
                     HIPFVEEWEAEIGKR"
     gene            2017..3234
                     /locus_tag="PKO06_08155"
     CDS             2017..3234
                     /locus_tag="PKO06_08155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV69653.1"
                     /translation="MVHQASTDSPVRESLIAVASFLVIFAFSTVDSAVSPLVQVLNEY
                     FQVGEARTLYLVTACTTGTVLGILIGPALTAAYRIDMLLNLAMAGLVAGLALFLVSKD
                     FVFALATRAVFGISSGLFASCMWWVTFYGVSALGREKMITVLMSARPLATALGVPLIG
                     MAAETWDWKTSLWGCFFVLVVSGLWLLVESRGLHNSSLESFSPSKVFRDYLIALREPR
                     ASQYYLGFTLNRMCYFGFYVMAGIWFRRHFGTSLVEVSQALLLIGVAEAGINFVVPAI
                     RRRIGHRLAFHGCLWASMVLFPAFVFGSMSYPVTLALIAVFMVLDRIYCMSLVITLPD
                     MFPTAPNRTVLGSLNTLTAWTALSTISFLQGRFLETWGMPAMQSLLWVVLVVGSVMLI
                     RVQNLTFSAEPAK"
     gene            3542..>3901
                     /locus_tag="PKO06_08160"
     CDS             3542..>3901
                     /locus_tag="PKO06_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69654.1"
                     /translation="MHTWKKNAFIMVVAFMMSFAAEVMAGLAPRDEDPVAVQRKTISD
                     FDGECTVTVEWLRRPGEFALTIDYFGYLTQEGRVNLYLEVNGARREFLTLTEELPNRH
                     QRIRILSFHPMTGKKGPN"
BASE COUNT          762 a         1059 c         1178 g          902 t
ORIGIN      
        1 atgagcggcg gtggcctggg aaatctcgcc ggagcgttcg acgaactgat gtttctgttg
       61 cccgacttct atcagggtgg gcgggtggtc gtcgagccca tgatggattt taccgcgacc
      121 ctgggcactc tggttcagat cgaagacagc ggttgtcgat tcgaaggcat cgatcagcag
      181 atgatcgaag acagcggctg ggtaggattc cggtatccgt ggtctgaaca gcccgtagcg
      241 gatcgactgc gggaactttc gcttcgcatc ggacaagaag ctcatcaaat gggcgctcga
      301 ggctatctca acctggactg gggcattctg gatggcggtg gtgagttgaa gatccacgcc
      361 ctggaatcca acttccggca caatggcttg tcgcatctgc tcgatttacg gcgtcgctat
      421 ttcggcgaag cgggacactc gctgcacatc attctgcgcg agaccttccg tctgccgggc
      481 agtgaactga cctgggaaca actggtacaa cgactcgagt cggtcagaca cgaggacgaa
      541 ccactgctca ttgccggccc tggacgcgaa ttcggcgtga ttcccgtgtt accgcccgcg
      601 aatcacacct gtggcatcgc ggttttcgga agatcgcagg cagaggctga aaccctgctg
      661 cagaaggtct atcgcgcact ggcctgacga gttctgcgtc tggcgtctgc ggtgatggtc
      721 gatcgacacc gggctgactg tctgcgctga cccgcctcta gcgcttgccg atctctgctt
      781 cccattcctc gacgaaggga atgtgcggga cgtcgttata aatgacgccg accccgagag
      841 cgtgacaggc ttgcgctttg cgatcggtga ccaggcattc ctgcatcgga aaaagctgga
      901 tcgtcaggaa caggcgcgag agttctgctg tatcggcact ctgtcgcaga tagcggtctg
      961 catgcagcgc ggtcagaccg agcgactcga gcatgtgtct ggtcatggcc aggtgacagt
     1021 ctgcctggtt ttcgagtcga acgacggtct gcagcagatc aaaggtcatc tgtgccagat
     1081 cgcggaagcg gttggcggaa acgagcttca ggccttgttt ctgcagagaa cgcagcgaga
     1141 agcgcaaacg ctctgcttca ccggctgaca ggctgcgatg atgaatcggt gcccgatcga
     1201 ggggggcgat ggtgttcatg gaaaccaggt cgccgcagat gatggggata ccctgttttt
     1261 gaaagcgacg tgcccgacag tcgatgtccc aggcaatcgg caggttcaga cgctgcaagc
     1321 gggcaatctt gcgaaacagc ggagtcgaga gaccgccggt cagacgcgca tactgcggca
     1381 ggcgccgctc gtgactgcga attgcttcat cgagatgctc atacagaccg ccttttgccg
     1441 ctcgagaacc cggacaccag agctgattta tgggtgtcga gccaagacga tgtgcagcgc
     1501 tgaaccgatg ccgttcgagc aggttggcga aatcatgttg cagaacggcg ttcgcagggt
     1561 gctgctcgag ctggttcagg gaatcgagcg cggaaatcag cgcctggtcc tcaacacggc
     1621 ggtctcctgc cgctcgggct ctttctgcct gttgcagagc acgggcaaaa ccaccgagac
     1681 gcaggtgttc gaggggcgcg gggatcgtgg cctgttcacc acagagagag gtctcagcgc
     1741 tggccaggct ggcgccgaaa gccaacacca gaaccgtcag agacagactg cgaaagaatg
     1801 cgaccatgcg atccccccga ttgtatccat ggccatgaaa gccatgcatg gatttccctg
     1861 cgttttcggt cttttgatac tttactcacg gaactcaggt gtgtcaaatt gcacggtcgt
     1921 cgaaggtggg gcagcgaaac ccatcatgcg actcgtgtat cagaccgggg tgtgagataa
     1981 tcaagccatt catctccctc tcaggtcatt ccgagaatgg ttcatcaggc atcgactgat
     2041 tccccggttc gtgaatcgct gattgcagtg gcctcctttc tggtcatttt tgcgttttcc
     2101 acggttgatt cagcggtttc gccgctcgtg caggttttga acgagtactt tcaggttggt
     2161 gaggcgcgta ccctgtatct cgtgacggca tgcacgaccg gaacggtgct gggaattctg
     2221 atcggacctg cgttgacggc ggcgtatcgg atcgacatgc tgctgaacct ggcgatggcc
     2281 ggcctggtgg caggtctggc gctatttctt gtctccaagg actttgtctt cgcgttggca
     2341 acacgggcgg tgttcggcat ctcgtccgga cttttcgcat cgtgtatgtg gtgggtcacg
     2401 ttctatggcg tcagtgcgct cggtcgggaa aaaatgatca cggttctgat gtcggcgcga
     2461 cctctggcga cggctctggg agttcctctg atcggcatgg ccgccgaaac ctgggactgg
     2521 aagacctcgc tgtgggggtg ctttttcgtg ctggtggtct ctggtctctg gctgctcgtg
     2581 gaatcacgag gcctgcacaa cagctctctc gaatcattct ctccgagcaa agtgttccgg
     2641 gactatctca tcgcgctcag ggagccgcgg gccagccagt actaccttgg gttcacgctg
     2701 aatcggatgt gctactttgg tttttatgtc atggccggaa tctggtttcg acggcacttc
     2761 ggcacatccc tggttgaagt cagccaggct cttctgctga tcggcgtggc cgaagccgga
     2821 atcaacttcg tcgtcccggc cattcggcgt cgtatcgggc atcgcctggc ttttcacggt
     2881 tgcctctggg cttcaatggt gctgttcccg gcgtttgtgt tcggttcgat gagctacccg
     2941 gtgacgctgg cactgattgc cgtcttcatg gttttggacc gcatctattg catgtctctg
     3001 gtcatcaccc tgcctgacat gtttccgacc gcaccaaatc gaactgttct cggcagtctc
     3061 aacacgttga cggcatggac tgctctctcg acgatctcct ttctgcaggg gcgttttctc
     3121 gaaacctggg ggatgcctgc tatgcagtct ctgctctggg tcgtgcttgt cgtcggctcg
     3181 gtgatgctga tacgagtgca gaatctgacc ttcagcgctg agccggcgaa atgagcttcc
     3241 tgagcgaacg attcccgatc cgaagcactt tttgagcaaa aactgggcag tctcagaccc
     3301 ggcatcagga aagaatgtca ggcccctgat gcagcgcaga ccctgcattg tcacgtgccc
     3361 tcatgatcaa atgctgtaca gttcgcgcca gaaccccatg acctgaaaga catcggtgag
     3421 ctgcgaaacc tgcttccaaa gagcctttta ccagaatgag atcgaagaaa gtaccacctg
     3481 gcattgaata tgcggagctg tcgccaatcg aactcgttct gctctgtctg gaggtacgaa
     3541 catgcatacg tggaagaaga atgcttttat catggtggtg gcgttcatga tgagttttgc
     3601 ggcagaggtt atggccggtc tggctcctcg agacgaagat cccgtcgcgg tacaacgaaa
     3661 gacgatctcg gacttcgatg gtgaatgcac cgtcacggtc gaatggctgc gccgcccggg
     3721 agagtttgcc ctgactatcg attacttcgg ctatctcacg caggaaggtc gggtcaatct
     3781 gtacctcgaa gtgaatgggg ctcgccgcga gtttctgacc ctgaccgaag aactgccgaa
     3841 tcggcatcag cgcattcgca ttctgtcgtt tcatccgatg accggcaaga aagggccgaa
     3901 t
//
LOCUS       DAORHD010000328         3898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25465_length_3898_cov_2.535519, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000328 DAORHD010000000
VERSION     DAORHD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25465_length_3898_cov_2.535519"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PKO06_08165"
     CDS             complement(<1..1261)
                     /locus_tag="PKO06_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69655.1"
                     /translation="MEHQNVLKPTQTTIHWSIRLCFHYVFWSLFLEGVIRKWVLPGSS
                     NLVFFMKDPAVLIIYLYFLRRGLMPGKMPFTSFFYLLTFFGFVSVFQPQPGLIFNYLG
                     LYGWRNYFFFVPLAFIIAEYLPKPTLYSILRQFLWFSIPIAVLVFLQHNAPGGAVINA
                     GISGAFGSLPVSRGFIRPNGPFSFVHLMNCYNLILWGCILGLWAMRAQERPVSRYLLV
                     AAALSGVASVAVSGSRAAVAGVGLVALFLFLGISVREGIGKGSFFSSTLSSVTFLFAT
                     GYIALQFFYEPLEGLRERAESIVALEERMGLGWGWGFGLINRFFYDMISFWQQFSQVP
                     ILGNGLGLSSTAAIVFGTNPGGIENDWDRHIYELGPVMGVGFIFLRVWITVWLFKRTL
                     EGVSHGTGILGFLIVGGIFHTVLLGQLG"
     gene            complement(1263..2294)
                     /locus_tag="PKO06_08170"
     CDS             complement(1263..2294)
                     /locus_tag="PKO06_08170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV69656.1"
                     /translation="MNLLIGSYSYYPNIGGLERITNMMAHAFQKQGHAVRIVTQTHGR
                     AGADDAADIPVVRRPSIPAFLDCCRWADLLWHNNLSLRFGLPFLLTRKPQIITTQTFY
                     GGEPAYEDWVFKCKRRFIKRFPHVILCSEMKRQFPDGVVIPNPFETSMFSQATRPVRD
                     RWFVFVGRLVSDKGVDDLLQAVASLRERGVLAPVTIIGEGPEELKLRGFVERVQLQSL
                     IHFTGALQGQALAHEISRHRCLVVPSRWREPYGIVALEGLACGCWVIGSAGGGLPEAI
                     GPGGWTYPNGDCEALAGLLQRFIETGENLSLETRGQISLHLNQRTVEHIAELYLGVFN
                     REILRAQGR"
     gene            complement(2291..3229)
                     /locus_tag="PKO06_08175"
     CDS             complement(2291..3229)
                     /locus_tag="PKO06_08175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV69657.1"
                     /translation="MYRHLDWRISRSLSNSSNITSVYLYEDGACRSFRLASELGLERV
                     YDLPIPYWEVLHRWMTAEAERWPEWSMTIPAMMDSRQKLLRKSEEVTLANKIVVASTF
                     TLRSLDGKTRPDQSIRIIPYGCPPPVSLDQCQRSQSGILKLLYVGSLTQRKGLAYLQA
                     ALNQVGWRHLEVTIIGRRPEAVCPPLEKLLARCTHIPTLPHAEILSQMRRHDLLVFPS
                     IAEGFGLVISEALSQGLPVLTTENTAGPDLIGRDVGGWVVPPGSTEALVEKIELALMR
                     PSLLREKGEQAVHVARSHGWEQYRRNLCSFVLGVGA"
     gene            complement(3714..>3898)
                     /locus_tag="PKO06_08180"
     CDS             complement(3714..>3898)
                     /locus_tag="PKO06_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69658.1"
                     /translation="PIIGIITVYCGRILMRQGISFFTQNTVLTFWRVETVSSFFDQNM
                     SRAWVLLLKAKKTFGA"
BASE COUNT         1065 a         1128 c          954 g          751 t
ORIGIN      
        1 cccccagctg accgagtaaa acggtgtgaa agataccacc gacaatgaga aaaccgagga
       61 ttcccgtgcc gtgggagaca ccctctaacg tccgtttgaa caaccagaca gtgatccaga
      121 ctctcaagaa gataaaaccc acccccatca caggcccgag ttcgtagatg tgacggtccc
      181 agtcgttttc gatgccacca ggatttgtgc caaacacaat cgccgccgtt gacgataagc
      241 caagcccgtt gcccaaaatc ggcacctggg aaaactgctg ccagaaagag atcatatcgt
      301 agaaaaagcg attgatcagg ccaaaacccc aaccccaacc cagccccatc ctctcttcca
      361 aagcgacaat actttctgca cgctctcgca acccctcgag aggttcatag aagaactgca
      421 gcgcaatata ccccgtcgca aacagaaagg tcacagaaga cagcgtgctg gaaaaaaacg
      481 aacccttgcc gattccttca cgcacactga ttcccagaaa cagaaacagg gcgaccaaac
      541 caaccccggc cacagcggca cgactgccac tcaccgccac gctcgccacc cccgagagcg
      601 ctgcagcgac aagaagatac cgagaaacag ggcgttcctg agcgcgcatg gcccataacc
      661 ccaagatgca tccccagagg atgaggttgt agcagttcat gagatgaacg aacgaaaatg
      721 gtccgtttgg gcgaatgaac ccgcgagaca ccggaagact gccaaaagcg cccgaaatgc
      781 cggcattgat caccgcgcca ccaggggcat tatgctgaag aaacaccagc accgcaatag
      841 gaatggagaa ccagagaaat tgcctgagaa tcgaatacaa agtcggcttc ggcagatact
      901 cggcaatgat aaatgccagg ggaacaaaga aaaaatagtt gcgccagccg tacaagccaa
      961 ggtagttgaa aatgaggcca ggttgaggct gaaagaccga tacaaacccg aaaaacgtca
     1021 ataggtagaa gaagctcgtg aacggcattt tcccgggcat aagccctcta cgcaaaaaat
     1081 agagatagat gatgagtact gccggatctt tcatgaaaaa aaccagattc gaggagcccg
     1141 ggaggaccca tttacgaatg actccctcca gaaacaacga ccagaaaaca taatggaaac
     1201 acaggcggat actccagtgt atcgtggtct gcgtcggttt cagcacgttc tgatgttcca
     1261 cgttatctgc cttgagcccg caagatttct cggttgaaaa caccaagata gagttccgca
     1321 atgtgttcga cggttcgctg gttcaagtgt agcgaaatct gccctctcgt ttcaagtgac
     1381 aagttttccc cagtttcgat aaacctctgg aggagacctg ccagagcctc acagtcaccg
     1441 ttcggataag tccagccacc cgggccgatg gcttctggca atccaccacc cgcggaaccg
     1501 atcacccaac atccacaggc gagtccctca agggccacta taccgtacgg ctctcgccag
     1561 cgtgacggga cgaccaggca acggtgtcgc gagatctcat gagcgagtgc ctgaccttgc
     1621 agagctcccg taaagtggat gagagactgt agctgaactc gttcgacgaa cccacgcaac
     1681 ttcaattctt ccggtccttc accaataatc gtgacaggcg ccaggacacc gcgttctcgc
     1741 agactcgcca ccgcctgcag cagatcgtcc acccctttgt cagaaaccaa ccggccaacg
     1801 aatacaaacc accgatcacg aacaggccgt gtcgcctgcg aaaacatact tgtttcgaac
     1861 gggttcggga tcaccacccc atcagggaac tgtcgcttca tttccgaaca aagaatgacg
     1921 tgtggaaaac gcttgatgaa tcgccttttg catttgaaaa cccagtcttc atacgctggc
     1981 tctcctccgt aaaacgtttg tgtcgtgatg atctgaggct ttcgtgtcag cagaaaaggc
     2041 aagccgaatc gcaggctgag attgttgtgc cacaacagat cggcccatcg acagcagtca
     2101 agaaaagcag gtatcgacgg gcggcgaaca accggaatgt ccgcggcatc gtcggcccca
     2161 gctctgccgt gcgtctgggt gacgattcgc acggcatgtc cttgtttctg aaaagcatgc
     2221 gccatcatgt tcgttattcg ctcaaggccg ccaatgttgg gataatagga atatgatcca
     2281 atcagcaaat tcatgcgcct actcccagga cgaacgaaca caagttcctt cgatactgtt
     2341 cccacccgtg agaacgtgca acgtgaaccg cctgctctcc tttttcacgc aagagactgg
     2401 gtcgcatcaa cgccaactct atcttctcga caagcgcttc tgttgaacct ggaggaacca
     2461 cccacccccc gacgtcgcgc ccgatgaggt ccggtccggc agtgttttct gtcgtcagga
     2521 ctggaaggcc ttgtgagagt gcttcggaaa tcaccaaccc gaacccttcg gcaatcgaag
     2581 gaaagaccag cagatcgtgt cttcgcatct ggctcaggat ctccgcgtgc ggcagggtcg
     2641 gaatatgcgt gcaccgtgcc agcaacttct ccaggggcgg gcagacggct tctggtcgtc
     2701 ggccaatgat ggtgacctcg agatgacgcc aaccaacctg gttcaaggcg gcctgaagat
     2761 aggcgagtcc ctttcgctga gtcagactcc ccacatacag caacttcaaa atgcctgact
     2821 gggacctctg gcattggtcg agagaaacgg gaggaggaca tccgtacgga atgattcgaa
     2881 tggactggtc aggacgagtc tttccatcaa gcgatcgcag ggtgaaggta ctcgccacca
     2941 caattttgtt ggcaagcgtc acctcttcag acttgcggag cagtttttgc cgactatcca
     3001 tcatcgccgg gatggtcatg ctccattcgg gccaccgttc tgcttcggct gtcatccatc
     3061 ggtgcaagac ttcccaatac gggatgggca gatcatacac ccgctcaaga ccaagctctg
     3121 aagccagccg aaacgatcga cacgcaccgt cttcatagag atacacgctc gtgatgttcg
     3181 aactattcga aagcgaacga gagatgcgcc agtcaagatg ccgatacacc tgatcgacac
     3241 agaaaggacc agattcatga cgaacccagg aacgacacca cggcgacgtc aggagaagac
     3301 gcgcacattc cctcaccggg tcaagccgta aaaccccatc gtgcaatact cccagcgatc
     3361 gacgacccaa tctcgacccg atcgattcgc acgtcggaag ccacgctgga acattcaccc
     3421 agcccagaga ggtccagaac tcccgaagca agccggcttc gtccaatgtc tgcagggcct
     3481 gccgaaagaa ctcattcccc gttgggtgag ctatcaagac gcctggattt ctatgagctt
     3541 tctttgggag catttgacaa cagggatcta attcaccctc acgagaatga atgtcacgag
     3601 atctgatcgg cgctccctct tcaactttag agacagcctg gcaaactgcc tgaattcttc
     3661 acgcgacaaa gaaacgtcat ctgccgcatg aaaaacgcgt tctcctgaaa gattcatgcg
     3721 ccgaatgttt ttttcgcttt caacaagaga acccaggcac gactcatatt ctgatcgaag
     3781 aatgacgaaa ccgtttcgac gcgccagaag gtcaatactg tgttctgtgt gaagaaagat
     3841 atgccttggc gcatcaagat tcgaccacaa tataccgtaa tgattccaat gataggag
//
LOCUS       DAORHD010000329         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25538_length_3889_cov_4.141106, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000329 DAORHD010000000
VERSION     DAORHD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25538_length_3889_cov_4.141106"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..566
                     /locus_tag="PKO06_08185"
     CDS             90..566
                     /locus_tag="PKO06_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69659.1"
                     /translation="MRFGSYDITLSQIIYRCRQFVSVLRPQIDQTLVDEACHHLPKQW
                     QPSFRQIPSSTMAHILRLYRAIRDDAALDESTRSELLILALTHDIGKSITRPTLFERV
                     AKVLLPLPNKSHVIAGAMLMKKLGASRQLVRRIRTHHESVVDDRLLALFQTYDDRL"
     gene            complement(634..1584)
                     /locus_tag="PKO06_08190"
     CDS             complement(634..1584)
                     /locus_tag="PKO06_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNV69660.1"
                     /translation="MDKKAKIYVAGHSGLVGTAMRKTLQRQGYDNLLCKTFQECDLRD
                     GAQVEALFARERPDYVILAAAKVGGIHANNTYPAEFIHDNLMIQSHVIHQSWKHGVKR
                     LLFLGSACIYPRDCPQPIREEYLLSGPLEKTNEPYAIAKIAGLVQVESYNRQYGTRYI
                     SVMPNNLYGPGDNYDLQNSHVLPALIRKFHEAKVAGAASVEVWGTGTPRREFVFSEDM
                     ASACVYLLNQSDDSLFRGGHQAFFNIGSGQERTIRELAELVQKVIGFSGIIHWNTDKP
                     DGTPRKLVDMSRWKDFDWKPAVTLEQGIALAYEDFKQRFA"
     gene            complement(1609..2529)
                     /locus_tag="PKO06_08195"
     CDS             complement(1609..2529)
                     /locus_tag="PKO06_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV69661.1"
                     /translation="MNSSRYEFTFRRGGFGGDAEDVQPGGPIGLNGFRKLWIILSVIA
                     AMYLVHVWLSGPGYFFSLLLEYGLLIPIVIGSLTLHEYAHARLADFLGDPTPRLEGRV
                     SVDPRRHLDFLGTLLLFTAGFGWAKPVPVNPGYFRVPDRAMFFVSAAGPLTNLVLAVL
                     GGFLAAFGVSMLIPQWGPDIGKLLKTSFTDGIPLSVVLVRFASIGASFSEMLGHPGMR
                     TVIFLTFVQSLMTINLSLALFNILPVHPLDGSHILSFFLTPAQRFKLRQNAGTLSIVL
                     FMVVAFGLLGVVLNPAVKFLYRSILTLYGI"
     gene            complement(2642..>3889)
                     /locus_tag="PKO06_08200"
     CDS             complement(2642..>3889)
                     /locus_tag="PKO06_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69662.1"
                     /translation="LVHHLRRIGEHARDRSQQVILVGGCVRDLLLEQRNLDMDVVVVG
                     NAVEFAQSLQEKHGGELVHFAQFHTARWVVEGLKIDFSSARIEHYAEVAALPKVELAG
                     LTNDLYRRDFSMNALAIDLLPERFLQVQDFFGGYQDLKARRIRIMHPFSFMEDPTRLY
                     RAIRFAARFSFELAEETRRAFDLAIERGVVAKLSYKRLGAEISRCFHEEKAYRALEML
                     FDSGLLKFLHRDLSRASLLPSRFRLIPGIVRRFQVLKEEIDSEAVFWAGLLSPLTPDQ
                     AEELLVSMGQPGERRDAAVAALRGWRTLPVQLARLAPDDDVGLYDALIPFPLAALVAL
                     VAFTLDKHAVRRVFDFLGRLRPIRLAISGKDLIAVGIPTGPMMGRVLRGVLRERIAGG
                     VLTAAEQIARARELCQCYSTEPA"
BASE COUNT          942 a         1157 c         1053 g          737 t
ORIGIN      
        1 aaatgatcgg caaggaaaga gccactcgtg agcgtctgct gcagatgtgg ccggaacgct
       61 accgcaagtc gggcaaagga aactgacaga tgcgcttcgg ctcatatgac atcacgctgt
      121 cgcagatcat ctatcgctgc cgacagtttg tttcggtact tcggccccag atcgaccaga
      181 ctctggtcga tgaagcctgc catcacctcc cgaaacagtg gcaaccgtca tttcgccaga
      241 ttccatcctc gaccatggca cacattctgc gattgtatcg agcgattcgc gacgatgccg
      301 cgctcgacga gagtactcgt tcagaacttc tcatactggc tctcactcac gatatcggca
      361 agagcattac ccgaccgacg ctattcgagc gcgtcgccaa agtgctgttg cccctgccaa
      421 acaagagcca cgtcatcgcc ggtgcgatgc tgatgaagaa actcggcgct tcacgccaac
      481 tggttcggcg cattcgcacc catcacgaat ccgtcgtcga cgatcgcttg ctcgccctgt
      541 tccagaccta cgacgaccgc ctgtaatcga tcgcgacaac aacgcaccca acagatgccc
      601 gctcaggcta tcgtctattc gcgcgcgacg ccttcaggca aagcgttgtt tgaagtcttc
      661 gtaggcgagg gcgatgccct gttcgagagt gacggcgggc ttccagtcga aatctttcca
      721 gcggctcata tcgacgagtt tgcgcggcgt gccgtcgggt ttgtcagtgt tccagtggat
      781 tataccggaa aaaccgatga ccttctgcac gagttcggcc agctcgcgaa tggttcgttc
      841 ctgacccgac ccgatgttga aaaaagcctg atgaccaccc cgaaacaggc tatcgtcaga
      901 ttgattcagc agatagacac aggcggaagc catatcttca ctgaacacga actcacgccg
      961 cggtgtaccg gttccccaga cctcaacgct tgctgctcca gccactttcg cctcgtgaaa
     1021 cttacgaatc aaggccggca agacgtgcga gttctgcagg tcgtagttgt caccgggccc
     1081 gtagagatta ttgggcatga cactgatata gcgtgttcca tattgccggt tataactctc
     1141 gacctgcacc agccctgcaa tcttggcgat cgcatagggt tcattggtct tctcgagagg
     1201 accggacaac aggtactctt ctcgaatcgg ctgagggcag tcgcgcggat agatgcaggc
     1261 tgaaccgagg aaaagcaaac gcttcacgcc gtgtttccag gactggtgga tgacatgact
     1321 ctgaatcatc agattgtcgt gaatgaattc ggcgggatag gtgttgttcg cgtgaatgcc
     1381 gccgaccttc gccgccgcta gaatgacata gtcgggtctt tcgcgggcaa acagagcctc
     1441 gacctgtgcg ccatcacgca ggtcgcactc ctggaaagtt ttgcagagca ggttgtcata
     1501 gccctggcgc tgcagggttt tacgcatcgc cgttccaacc aggccactgt ggccggcgac
     1561 gtaaatcttg gccttcttat ccatcattga tacgtcttct ttcacttttc agatgccgta
     1621 taacgtcaag atcgaccggt acagaaattt cacggccggg ttgagaacga cgccgagcaa
     1681 cccgaaggcc acgaccataa acagcacgat cgacagcgtg ccggcgttct gtcgcagttt
     1741 gaatcgctgc gccggagtca gaaagaaact gagaatgtgc gaaccgtcga gtgggtgaac
     1801 gggcaagatg ttgaagagcg ccagactgag gttaatcgtc atgagagact ggacaaacgt
     1861 cagaaagatg acggttcgca tacccgggtg ccccagcatt tcagagaacg aagccccgat
     1921 gctggcaaac ctgaccagaa caacgctcaa aggaatgcca tcagtgaagg atgttttcag
     1981 aagttttccg atatccggcc cccattgcgg gatcagcatg ctcaccccga acgccgccag
     2041 aaaaccaccc agcaccgcaa gaacaagatt cgttaacggg cctgccgcgg agacaaaaaa
     2101 catcgcccga tcgggaacac gaaaataccc gggattcacg ggtaccggct tcgcccaacc
     2161 gaaaccggcc gtgaacagca gcagggtgcc gagaaagtca aggtgacgac gcggatcgac
     2221 actcacccga ccttccagac gcggagtcgg atcgcccaga aagtcggcca gccgcgcatg
     2281 cgcatactcg tgcagagtta acgacccgat cacaatcgga atcagcaggc catattcgag
     2341 gagaagactg aagaaataac caggcccgga tagccagacg tgaaccagat acatcgcagc
     2401 gataaccgac agaatgatcc agagtttgcg aaagccgttg agcccgatcg ggccacctgg
     2461 ttgcacgtct tcggcatcac cgccgaagcc accccggcga aaggtaaatt catagcgtga
     2521 cgagttcatg atctcttgtc atatcacatt tcaccatcgc agaaaacccc gcaagccatg
     2581 ggaaacgggg ggaaatgcac acttttcctg cgtgggctcg ggctttgaac tcactcgtgt
     2641 ttcaagctgg ttcggtggaa tagcattggc agagttcgcg ggcccgggcg atctgttccg
     2701 cagccgtgag aacgccgccg gcaatgcgtt cgcgcagcac accgcgcagc acgcgaccca
     2761 tcatcggccc ggtcggaatt ccgacggcaa tcagatcttt cccggagatt gccagacgaa
     2821 tcggtcgcaa tcggccgaga aaatcgaaga cccggcgtac cgcatgcttg tcgagcgtaa
     2881 aggcgaccag cgccaccaac gctgccagcg gaaagggtat cagggcgtcg tagagcccga
     2941 catcgtcatc cggcgcgagg cgtgcgagct gcaccggcaa cgtgcgccaa ccgcgcagag
     3001 ccgccactgc cgcgtcacgc cgttcgccgg gctgacccat ggaaaccagc agttcttcgg
     3061 cctgatcggg tgtcaacggc gacaacaggc cggcccagaa gaccgcctcg ctatcaatct
     3121 cctccttcag cacctgaaag cgacgcacga ttcctgggat caaccgaaat cggctcggca
     3181 acagcgaagc gcgactcaga tctcgatgca gaaacttcag caatccacta tcaaagagca
     3241 tctcgagagc ccgatacgct ttttcctcgt ggaagcaccg ggagatttca gcccccaagc
     3301 gcttatagga aagtttggcc acaaccccac gctcgatcgc cagatcgaag gctcgacgcg
     3361 tttcttctgc aagttcgaac gaaaaccgag ccgcaaagcg aatggctcga tagagtcggg
     3421 tcgggtcctc catgaagctg aacgggtgca tgatacggat tcggcgagcc ttcagatcct
     3481 gatagccacc gaagaaatct tgaacctgta aaaaccgctc gggaagaaga tcgattgcca
     3541 aggcattcat ggaaaaatcg cgccggtaca agtcgttcgt cagaccagcc agttcgacct
     3601 tgggaagcgc ggccacttcc gcgtagtgtt cgatgcgcgc cgagctgaag tcgattttca
     3661 acccctcgac cacccaccgg gccgtatgaa actgggcaaa atgtaccagt tcgccgccgt
     3721 gcttttcctg cagggactgg gcaaactcaa cggcattccc gacaaccacg acgtccatgt
     3781 ccaggttacg ctgttccaga agaagatcgc gaacgcagcc ccccaccaga atcacctgct
     3841 gagagcggtc acgcgcatgc tcgccgatgc gacgcagatg atgcacgag
//
LOCUS       DAORHD010000330         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25632_length_3880_cov_3.606797, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000330 DAORHD010000000
VERSION     DAORHD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25632_length_3880_cov_3.606797"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1810
                     /locus_tag="PKO06_08205"
     CDS             146..1810
                     /locus_tag="PKO06_08205"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV69663.1"
                     /translation="MPVNTPDLRKYKLLDLIGCGGMGYVYAGYDAANNPVAIKILAPE
                     YADVEPVFKRFKLEGEILRGLNHPYIVKFVDMGQEGSYHYVAMEYVKGICLDSFPLSH
                     SATSCGVDQPIPPITDYLRIFIRCMEAMSFFHKKGLVHRDIKPQNIILQGEELMPKFI
                     DFGIAQKVEDDDSDPDAKPENLYTVVYASPEQLTNKSINIQSDLFSFGVVMYEKLTGK
                     LPFEGRTAMEVFLCHAKGNYAPPRQVNSEIPQKLEDIIVKLLQKDPAMRYPSATMVQG
                     ELERLLDVLQSGQKGLSMSGVIGEIRSGIPGVDRRVGKRISVSEEQNLLKKARNEFVE
                     AKNQLRSATSKYKTNPEQVQQLKLLCHTLQNQFEGLQEQVKMNLGFKSQPLILDRFNS
                     IIRMDKMVYEKRGISFTINTIEQKLTHTDGEDIIVGSINFTEKAKRFFSRNYRDTFLT
                     WDETNWFFQPYEEKHFPVFFMVGDRTLPHSPHGFRGLFWPFEFLVAIQKLGRTGVAIV
                     ETYRGVDRHEKAVFATHKEVILFSQSLIMQANAILAQMLKKGPAKA"
     gene            1807..2274
                     /locus_tag="PKO06_08210"
     CDS             1807..2274
                     /locus_tag="PKO06_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69664.1"
                     /translation="MKGTPELFFTILQSMTRLWENLSRDNRAFLDGSFEQASIQALLD
                     QRELVLEENRILEQRFLEEWRTRVPEHPLSRLSEALTILEQRYPAGTGALNAFRQALE
                     QLIESDGRVQEKLRQQRLNIETQIKSLKRSSTLVKGYKQASPFDSSCFIDKIR"
     gene            complement(2383..2709)
                     /locus_tag="PKO06_08215"
     CDS             complement(2383..2709)
                     /locus_tag="PKO06_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNV69665.1"
                     /translation="MFKGAPPNMQGLMKQAQKMQEKMQAAQEELKSRTVEESVGGGMI
                     TVVFTGAQELKKITINKEVVDPEDIETLQDLVLSAVNAGLKKAQDMMQEEMGKITGGL
                     NIPGLF"
     gene            complement(2777..>3880)
                     /locus_tag="PKO06_08220"
     CDS             complement(2777..>3880)
                     /locus_tag="PKO06_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69666.1"
                     /translation="RWPSGLRGSAVFSGLVDVLGQPVAVPGFDFVVSGTAGLNVFLDD
                     QFRQPLIGSVFSQNRLYVELIGPLSTGDPVNPVRLNAVTGRQATTTLALAMLDLASGS
                     RRFWGMLEINTPARSLPTLTVNLIPGERVTLSSPSLPNLARTLYFRAAGDSPPTVITG
                     AALYSERNYVQRIDSVFQTPTLFIEIAAEDRNWLFADTTRVKVFSDSDSAGFELVLQE
                     SDLHSGKYRGFITLDAKSSFGSANRLAVRPGERVTVTSVADPRVFTSARWVPENSLSN
                     VLPWPNPARGDRVHFQYRLAMPCKVDLTIYDTSGEEIYLATMMGQEGENRFTWVFPRR
                     TANGLYLFNLEMDPTLEFPTRKRRVRGKFAILR"
BASE COUNT          938 a         1167 c         1001 g          774 t
ORIGIN      
        1 gatgccaagc gcgagtggga agaagtgatc aagctcaagc ccaactcgaa gattgccaag
       61 aatgctcagg agcgcatgga gtatatcaaa gccaacaaac tggcttgatc gtgcgcctcc
      121 caacccaccg cagcccggat aaggaatgcc tgtaaatacc ccggacttac gaaagtacaa
      181 gctcctcgat ctcatcggct gcggcggaat gggatacgtc tatgccgggt atgatgccgc
      241 aaacaacccg gttgccatca agattctcgc gcccgagtat gcggatgtcg agcccgtctt
      301 caaacgtttc aaacttgaag gagaaatcct tcgcgggctg aatcatccgt acattgtcaa
      361 gttcgtcgac atgggccagg aaggctccta ccactatgtg gcgatggagt atgtcaaggg
      421 catctgtctc gactcgttcc cgctcagtca ctcggccacc tcgtgcgggg ttgatcagcc
      481 gatcccgccc atcaccgact acctccgcat cttcatccgg tgcatggaag cgatgtcctt
      541 ttttcacaaa aaggggctcg tgcatcgcga catcaaaccc cagaacatca ttcttcaggg
      601 cgaagagctc atgcccaaat tcatcgactt cggcattgct caaaaagtcg aagacgatga
      661 cagcgacccg gatgccaagc ccgagaactt gtacacggtc gtttacgcca gccccgagca
      721 actcacgaac aagagtatca acattcagtc agatctgttc tcgttcggcg tcgtgatgta
      781 cgagaagctc acggggaaac tgcccttcga aggccgaacg gcgatggaag tgtttctctg
      841 tcacgcgaaa ggcaactatg cgccgccgcg ccaggtgaac tccgagatcc cacagaaact
      901 cgaagacatc atcgttaaat tgctgcagaa agatccagcg atgcgctatc caagcgcgac
      961 gatggttcag ggagaactcg agcgtctgct cgacgtactg cagtccggtc agaagggcct
     1021 ctcgatgagc ggcgtgatcg gcgagatccg ttccggcatc cccggagtcg atcgacgcgt
     1081 cggcaagcgc atctccgtgt ccgaggaaca gaaccttctc aagaaggcca gaaacgagtt
     1141 tgtcgaggcc aagaatcaac tgcgttcagc aacctcaaag tacaaaacga atccggaaca
     1201 ggtgcagcaa ctgaaactcc tgtgccacac cctgcagaat cagtttgaag gtctgcagga
     1261 acaagtgaag atgaacctgg gcttcaagag ccagccactg attctcgatc ggttcaattc
     1321 catcatccgc atggacaaaa tggtctacga gaaacgcggc atctcattca ccatcaacac
     1381 catcgaacag aagcttaccc ataccgacgg cgaagacatc attgtcggct cgatcaactt
     1441 caccgaaaag gccaaacggt tcttctcgcg aaactaccgc gataccttcc tgacctggga
     1501 tgaaacgaac tggttctttc aaccatacga agaaaagcac tttccggttt tcttcatggt
     1561 tggtgaccgc accctccccc actccccgca cggcttccgt ggtctgttct ggccgttcga
     1621 attcctcgtc gccattcaga aactcggacg cacgggagtc gccatcgtcg aaacctatcg
     1681 cggggtcgac cgccacgaaa aagccgtttt cgccacccac aaggaagtca ttctcttctc
     1741 acagagcctg atcatgcagg ccaatgccat tctcgctcag atgttgaaga aaggccccgc
     1801 caaagcatga aagggacgcc ggaactcttc ttcaccattc tccagtccat gacccgactc
     1861 tgggaaaacc tcagtcgcga caaccgggca tttctcgatg gcagcttcga gcaagcttct
     1921 atccaagcgc ttcttgatca gcgcgaactc gtactcgaag aaaatcgcat ccttgagcag
     1981 cgttttctcg aggaatggcg aacccgggtt cccgagcatc cgctctcacg cctgagtgaa
     2041 gctctcacca ttctggaaca acgatatcca gccggcacag gagcgttgaa cgcgttccga
     2101 caagctctcg aacaactcat cgaaagcgac gggcgcgttc aggagaagct gcgacagcag
     2161 cgcctgaaca tcgagactca gatcaagtct ttgaaacgta gttcgacgct ggtcaagggg
     2221 tacaaacagg cctcgccctt cgacagcagc tgcttcatcg acaaaatccg ctgatcggca
     2281 gaagccgcct tcgcctcgga atagagcggc aaccgtcaat gaaaatcgca gtcgcggttg
     2341 tctattctcg aacgaaggcg gccgatggcg aaccgtaaca gatcagaaga gaccgggaat
     2401 attgagcccg cccgtgatct tgcccatctc ttcctgcatc atatcctggg ctttcttcaa
     2461 cccagcgttc accgctgaaa gcacgaggtc ttgaagggtt tcgatatctt cagggtccac
     2521 gacttccttg ttaatcgtga tcttcttcag ctcttgcgcg ccggtaaaaa cgacggtgat
     2581 catgccacca ccgacactct cttccaccgt tcgcgatttc agctcttcct gagccgcctg
     2641 catcttttcc tgcatcttct gcgcctgctt catcaggccc tgcatgttcg ggggagcgcc
     2701 cttgaacata tctgactcct tggacaaagt attagtgcca tcgcggcacg gtcattctac
     2761 cacactcccg cccacttcag cgcaggattg cgaactttcc acgaacgcga cgcttgcgcg
     2821 tgggaaactc gagcgtcggg tccatttcca gattgaacag gtacagacca ttagccgttc
     2881 ggcgcggaaa cacccatgtg aagcgatttt ccccttcctg gcccatcatg gtcgcgaggt
     2941 agatctcttc tcccgacgtg tcgtagatcg tcagatcgac cttgcagggc atggccagcc
     3001 gatactggaa gtgaacccgg tcaccgcgcg ccgggttcgg ccagggcagg acattcgaca
     3061 ggctgttttc gggaacccag cgggccgagg tgaacacacg cgggtcagcg acggacgtga
     3121 ccgttacccg ctcccctggt cgaaccgcca gacgattcgc cgaaccaaag ctgcttttgg
     3181 catcgagagt gatgaagccg cgatacttgc cggaatgcag atccgattcc tggagtacga
     3241 gttcaaaacc agcagaatca gagtcagaaa agacttttac gcgagttgta tcggcaaaca
     3301 gccagttccg atcctcggca gcaatctcga tgaacagcgt cggggtctgg aacacgctgt
     3361 cgatgcgctg aacatagttt ctttcagaat acagggccgc cccggtaatg accgtcggcg
     3421 gcgaatctcc ggcagccctg aaatagaggg ttcgcgcgag attcggcaga ctcgggctgc
     3481 tcagcgtcac ccgttcaccc ggaatcagat tcacggtcag ggtcggcagg ctacgcgccg
     3541 gcgtattgat ttccaacatg ccccagaatc gtctgctccc cgaggcgaga tcaagcatcg
     3601 cgagagcgag tgtcgtggtg gcctgacgcc cggtcaccgc attgagccga accggattca
     3661 cgggatcccc ggtgctgaga ggcccgataa gctccacata cagtcggttc tgggaaaaca
     3721 ccgatccaat cagaggctgc cggaactggt catccagaaa gacattgagc ccggctgtgc
     3781 cgctgaccac aaaatcgaat ccgggcaccg caacgggttg gcccagaaca tcgaccagcc
     3841 cggaaaacac cgcggatccc ctcagacccg acggccagcg
//
LOCUS       DAORHD010000331         3875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25683_length_3875_cov_3.499476, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000331 DAORHD010000000
VERSION     DAORHD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25683_length_3875_cov_3.499476"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKO06_08225"
     CDS             complement(<1..419)
                     /locus_tag="PKO06_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69667.1"
                     /translation="MNHSFRLTKVFHAAIVIGLLFTASSAWCRDFAWHPTSGAAADRV
                     VVPGDKPLRLGDVNVFLNCLEFGLGVALTDADVERIRTQFMEEYLQQKGRLLTAVREL
                     KNMWQAITTESSTEIGKYRRIIQDALLEEARRNPQLPL"
     gene            complement(457..1590)
                     /locus_tag="PKO06_08230"
     CDS             complement(457..1590)
                     /locus_tag="PKO06_08230"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69668.1"
                     /translation="MANPPSFQALFSYPEIQELMRSLGEGIVISDTEMRVLYLNPTAE
                     KLLNTTLEQIRGISLLGCHRNPSKVEELINMGELREPFRTEIQHGSHWLHVTATPLSI
                     EKGKHFGATMIVSDHTLRHQLEERLRQQFKELSERQRLLDTQIALARQIQKSLQPPEI
                     QAMPRASLRFWNTQSQVVGGDFAVASAPWIILGDVMGKGIFASQFVPLMIGFIGEETH
                     KTDSPAQLLGALNQRLCSFIGDRFSLFVSLAALRWMPESSSLEIAMAGHEAPTVFRVD
                     GTVQKLTQESVPLGVASSSTYFNSSVRLCDRDRVVLFSDGLTEPKVEQTYEQAVTRFI
                     DKYNEKLRLGQDAFSATINELGAEAVHLQPGDDQTMLELEVRE"
     gene            complement(1609..2328)
                     /gene="radC"
                     /locus_tag="PKO06_08235"
     CDS             complement(1609..2328)
                     /gene="radC"
                     /locus_tag="PKO06_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNV69669.1"
                     /translation="MKNPSVSDHAPGLISHHGHRARLKQRYDEVGLDGMAKHEAVELL
                     LTYVIPQKDVKPLAKRLLERFHDLPELFAARRESLLEVEGIGEQAARFLHVIRDLGQQ
                     ALKARAMHWGAALACPKDVVEYLRALMANLPEEEFVTIFVDHANRVIRYETLSRGVED
                     QTAVYPRKVMKRALALHATGIAVAHNHPTGNPQPSPADREITRHLAAAASTLELRLLD
                     HIIIGRDQQAYFSFRENGLLP"
     gene            2619..>3875
                     /locus_tag="PKO06_08240"
     CDS             2619..>3875
                     /locus_tag="PKO06_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69670.1"
                     /translation="MRLCVRLAVALSVFTLLMTVFAVPSAADPATPSWDVPTQPGKIS
                     ERLRYIPYDDLKDVLAKDPTWVIIPRTEYQTLKETKDAFLARKPASPEPWLGTEFVFG
                     VAHYQGVVKGRTAEFEARIPFTLGDQAWTLLPLPAGDLGLLTAEMDGKPAGTIARAFS
                     GIQDGEAPKGRVAVLQQKISDARRNRLTRDQSRRIGQANDFFLVVGGTGSHELKLRFV
                     VPLVDDPDKQELTFRIPRIPLSTFQIRLENADQVAEIDRAEGVTCRNEGKTVTVVDGA
                     LGSTDRFTLRWAPRTVTAVAPVPPAPEPDLTQPAPVEPVVATQAPVVRLPEEKPKVYA
                     DSYTLISIGDGFVRQEATVRVNIARAARGTISFLLPPGTDVIDIRSPRLESTQISRVG
                     SATRVLCRLNAKIQGNVEFTLVAETKI"
BASE COUNT          804 a         1045 c         1173 g          853 t
ORIGIN      
        1 agcggcagct ggggattgcg tcgtgcttct tcgagcagcg cgtcctgaat gatgcgacgg
       61 tatttgccga tctcggtcga gctctcggtg gtgatcgctt gccacatgtt tttcagctct
      121 cgaacggccg tcagcaggcg tcccttctgc tgcagatact cctccatgaa ctgtgtgcga
      181 atgcgttcga cgtcggcgtc ggtgagcgcg acgccgagcc cgaactcgag acagttcaga
      241 aacacgttga catcacccaa gcgcaggggt ttgtcgcccg gaacgacaac gcgatctgcg
      301 gccgcgccag aggtcggatg ccaggcaaag tcacgacacc aggctgacga agcggtgaac
      361 aggagaccga tgacgattgc agcatgaaaa accttcgtga gacggaacga gtggttcatg
      421 aactcctccc tttttcggca ttgtagcatg ggaacgctac tcgcgtactt ccaattcgag
      481 cattgtctga tcatctccgg gttgcaggtg tactgcttca gcacccagtt cgttgatggt
      541 cgctgaaaac gcatcctgac caagtcgcag cttctcgttg tacttgtcga taaaccgggt
      601 gaccgcctgt tcataggttt gctcgacctt gggttcggtc aggccgtcac tgaaaagcac
      661 gacgcggtct ctgtcacaca gtcggacgct ggaattgaaa taggtgctcg atgaagcgac
      721 acccagggga acggactcct gcgtcaattt ttggactgtg ccgtcgacac gaaacaccgt
      781 tggggcttca tgtccggcca tggcgatctc gagagaactg ctttccggca tccagcgcag
      841 ggcggcgagg gatacgaaca gggaaaaacg atccccgatg aaagagcaga gacgctggtt
      901 cagagctccc agcaactgcg cgggagaatc tgtcttgtgc gtttcctcgc caatgaagcc
      961 gatcatcaag ggaacgaact gggacgcaaa gattcctttg cccatgacgt cgccgaggat
     1021 gatccaggga gcggaagcca cggcaaaatc accgccgaca acctgtgact gggtgttcca
     1081 gaagcgaaga ctcgcacggg gcatcgcctg aatttccggc ggctgcaggc ttttttgaat
     1141 ctgacgagcc agggcgatct gcgtgtcgag caggcgctgg cgctcactca gttccttgaa
     1201 ctgctgtctc aaacgctctt cgagttggtg acgcaacgta tggtcagaga cgatcatggt
     1261 tgccccgaaa tgctttcctt tctcgatgga gagcggggtg gcggttacgt gcagccagtg
     1321 cgaaccgtgt tgtatctcgg tgcgaaaggg ttcgcgaagt tcacccatgt tgatcaattc
     1381 ttcaaccttt gacggattgc ggtgacagcc aagcaggctg atgccgcgaa tctgctcgag
     1441 ggttgtgttc agcagttttt cggcggtcgg gttgaggtag agaacacgca tctccgtgtc
     1501 ggaaatcacg attccttctc cgagagatcg catcagttcc tggatctcgg ggtaggaaaa
     1561 cagtgcctgg aaggagggag ggtttgccat aggattcagt tcactttatc agggcagtag
     1621 cccgttttcg cgaaagctga aatacgcctg ctggtcgcgc ccgatgatga tgtgatcgag
     1681 caggcgcagt tcgagtgttg aagccgccgc agccagatgg cgggtgatct ctcggtcagc
     1741 tggagagggc tgcggatttc cggttggatg gttatgagcg acggctatcc ccgtcgcatg
     1801 caacgccaga gcacgtttca tgactttgcg cggatagacg gctgtctggt cttcgacgcc
     1861 gcgagacagg gtttcgtagc ggatgacccg gttggcatga tcgacaaaga tcgtgacgaa
     1921 ttcctcttca ggcaggttcg ccatcagcgc acgcagatac tcgacgacat ctttcgggca
     1981 agccagggca gcgccccagt gcatcgctcg agccttgaga gcctgttgtc cgaggtcgcg
     2041 gatcacgtgc aggaagcggg ccgcctgttc gccaatgccc tcgacttcga gcaggctttc
     2101 tcgtcgtgcc gcgaagagtt cgggtagatc gtgaaagcgt tcgagcagac gcttggcgag
     2161 cggcttgacg tctttctgcg gaatcacgta cgtcaacagg agttcgacgg cttcatgttt
     2221 ggccattccg tccagtccga cttcgtcata ccgttgcttc aggcgcgcgc ggtgtccgtg
     2281 gtgagagatg aggcccggag cgtgatcgga aaccgatgga tttttcacga agctgatcac
     2341 tgcctctctg tgtctgaaat atgccctcgc atcttcaacg agatgccgcc catgcaggct
     2401 agcacagccg gtctggtcat gcaacgagca gaccgggggc acgagatctt tctgcatcgc
     2461 ctgtgcctga ttttttctgg tatggctcag tcggcaacgg atgataccgg aagttgccgg
     2521 gcaaaccgga ttcttgcaat tatggtactc gctgcataga atggaatcga actgctacga
     2581 gctgatcgga aggacatttc ccaactggga ggaatctcat gagattgtgc gtgcgtctgg
     2641 ctgtcgctct ttcggtgttc actctgttga tgacggtttt tgccgttccc agtgcggcag
     2701 accctgcgac tccatcgtgg gatgtcccca ctcaacccgg aaaaatctct gaacgtctgc
     2761 gttacattcc ctacgatgat ctcaaagatg ttctggcaaa ggatccaacg tgggtcatta
     2821 ttccgcgaac ggagtatcag acgctcaagg aaacgaaaga tgcgtttttg gcgcgcaagc
     2881 ctgcaagccc tgaaccctgg ctgggtacag agtttgtttt cggagtggcg cattatcagg
     2941 gggtcgtgaa agggcgaacg gccgagttcg aagcccggat tccctttacc ctgggagacc
     3001 aggcctggac cttgctcccg ttgccggcag gtgatctggg gttgctcacg gcagagatgg
     3061 atggaaaacc cgcgggaacg attgcccgag cattctcagg gatacaggat ggcgaggctc
     3121 ccaagggccg ggtggcggtt ctgcagcaga agatctctga tgcccgacgc aaccgtctga
     3181 cacgggatca atcccgtcga ataggccagg cgaacgattt ctttctggtt gtgggtggta
     3241 ccggcagtca cgaactgaag ttgcgatttg tggtaccgct ggtcgatgat ccagacaagc
     3301 aggaactgac cttccgcatt ccacgaattc cgctcagcac ctttcagatt cgcctcgaaa
     3361 atgcagatca ggttgcggag atcgatcgtg ccgaaggagt gacctgtcgc aatgaaggaa
     3421 agacggtcac cgtcgtcgac ggagccctgg gatcgacgga ccgcttcacg cttcgctggg
     3481 ccccacgtac ggtcacggcg gttgcgcccg taccgcccgc ccccgagcct gatctgacgc
     3541 agcccgcgcc ggtcgaacct gtcgtggcga cgcaagcccc cgtagtacga ttgccagaag
     3601 aaaagccgaa ggtctatgcc gacagctata cgctcatttc catcggcgac ggttttgtcc
     3661 gtcaggaagc gacagtacgg gtgaacattg cccgcgccgc tcgagggacg atcagttttc
     3721 tgttgccgcc cggcactgat gttatcgaca ttcgctctcc gcgactcgaa agcacgcaga
     3781 tcagtcgggt gggctcggcg acgcgagtct tgtgtcgtct gaatgcgaaa attcagggaa
     3841 atgtcgagtt cacgctggtc gctgagacga agatt
//
LOCUS       DAORHD010000332         3852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25907_length_3852_cov_3.227285, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000332 DAORHD010000000
VERSION     DAORHD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25907_length_3852_cov_3.227285"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKO06_08245"
     CDS             complement(<1..1144)
                     /locus_tag="PKO06_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV69671.1"
                     /translation="MSQPISAPTVPSTSTPAPAAATPAAKTATPVVQEPPLKLLVTST
                     PHVFSSDTVARIMWEVNAALLPALLVAWYVFGARALAVTAFSILGALVGEVVVQKFFL
                     GKPASPCDGSAIVAGLLLAFCVPAALPLWIAFLGGLMAIVFGKQVFGGLGQNVFNPAH
                     IGRAILLASWPVYMTTWVKPCTATGMCGALPDGVTMATPLGFMKEASRNPELAQKIKT
                     AGISALDYMFQTLHLGWNDLLVGTMPGCIGETAKIALIIGGLYLFLRGHIPWTTPTAM
                     MATVFVGSMLATGDPRLALFHLLAGGLIVGALFMATDMVTSPMTRTGQLIFGFGCGLI
                     TLIIRFKGGYPEGVCYSILIMNAFVPLIDKFTVPRRFGEIVATPAEV"
     gene            complement(1141..2505)
                     /gene="rsxC"
                     /locus_tag="PKO06_08250"
     CDS             complement(1141..2505)
                     /gene="rsxC"
                     /locus_tag="PKO06_08250"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNV69672.1"
                     /translation="MTETFAFSGGVHPPELKKTAGCSTIELPWPNKVVLPVSQHIGAP
                     AKPLVAVNDKVTCGQKIAEATGMVSAPVHASISGRIAGVGNFLHPFGSPALAIVIEQT
                     PEAEKPAFRVKNDGLLMPVDELKKRIQDAGLVGMGGATFPTHVKVSPPPTKPIDTVIL
                     NGVECEPALTSDHRLMLEQPDKVLIGLKLLMKVLGAKNGYIGIEENKPDAIALFTEKC
                     RHESHIRVAPLHVCYPQGGEKQLIYAVLQREVPSRGLPMDVGVVVQNVATAAAIYDAI
                     YMEKPVVDRIVTLAGNAVAKPGNYLVPMGMLIGDFVQAAGGFKPDRPPKKIIMGGPMM
                     GHALFNLQVPVVKGTSGLLCWDEEEAGEAPEPQVCIRCGRCVEGCPMRLMPSRIKGCV
                     DFNRLEEAERFGIGDCIECGCCGYVCPARLPLVQTFKLGKRRIADVNKRKAAADKAKQ
                     GSKA"
     gene            2722..>3852
                     /locus_tag="PKO06_08255"
     CDS             2722..>3852
                     /locus_tag="PKO06_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69673.1"
                     /translation="MIPVTTVRVSLLMLLCCLLSSLVLPLPAAAANRADLWKKVEKAI
                     NEGLPKTAIEHLTPIVDAALQDKAYGEAARAMCRKIVLEANIQGNKAEEKVTRLQTTL
                     ETAPGELKPLLRAVLARWYWHFFRQNNWRFMNRTATAKLDEKDFTTWDLPKLFGHIGG
                     LFDAAIADKASLQALSIKEFNEFLSSGNMPAEYRPTLYDFLVYQALEFYTSGEQAGAY
                     PQDAFFVEADSPALADASAFMAWNPATTDTDSPKLKAIRLLQDLLKFHAKDKDRGAFL
                     DADIYRLRWLKSVAVGENAAEACFKQLEVIAREFADHQLSSTAFEAMAQIRFAEGNRK
                     EAHQLASEGSKRFPNSAGGHNCRSLISQIEQKLSDPEFYKTGG"
BASE COUNT          856 a         1153 c         1081 g          762 t
ORIGIN      
        1 tcacctccgc cggtgtggcc acgatttcac cgaaccgccg aggaacggtg aacttgtcga
       61 tgagcggcac aaaggcattc atgatcagaa tcgaataaca caccccttca gggtagccac
      121 ccttgaagcg gatgatgagt gtaatgagac cacacccgaa gccgaagatc agctgtccgg
      181 ttctggtcat ggggctggtg accatgtctg tcgccataaa cagcgcgccg acaatcagcc
      241 cccccgccag cagatggaac aacgccagac gcggatcacc cgttgccagc atcgaaccga
      301 cgaacacggt cgccatcatg gcggtcggtg tcgtccaggg aatatgtccg cgcaggaaga
      361 gatagagccc gccgatgatc agggcgatct tggcggtttc cccgatacac ccgggcatgg
      421 tgccgaccaa cagatcgttc cagcccagat gtagagtctg gaacatgtag tcgagcgcgg
      481 aaatccccgc ggtcttgatc ttctgagcca gttccggatt gcgtgatgct tccttcatga
      541 aaccaagcgg ggtggccatc gtgactccgt cgggcagagc accgcacatt cccgtggccg
      601 tgcagggttt gacccaggtg gtcatgtaaa cgggccagct ggccaacaaa atggcgcgac
      661 caatgtgcgc cggattgaag acgttctgtc cgagtccacc gaagacctgc ttgccaaaaa
      721 caatcgccat cagaccgccc agaaaggcga tccagagagg caatgcggca ggaacacaga
      781 aagcgagcag cagaccggcg actatcgcac tgccatcaca gggagaggct ggtttcccca
      841 gaaagaactt ttgaaccacc acttcgccaa cgagcgcacc cagaatggag aatgcggtaa
      901 ccgccagcgc gcgcgcaccg aacacatacc aggccacaag cagagcgggc agcaacgcgg
      961 cattcacttc ccacatgata cgggcgaccg tatcggagct gaacacgtgt ggggtcgagg
     1021 tgaccagcag tttcaaagga ggttcctgaa cgaccggagt cgcggttttc gcagcagggg
     1081 ttgccgctgc cggagcggga gtggaagttg atggaaccgt tggagcggaa atgggttgac
     1141 tcatgctttc gatccttgct ttgccttgtc ggcagcggct ttgcgcttat tgacgtcggc
     1201 aatgcgccgc ttgccaagct tgaacgtctg taccagtggc aatcgagccg gacacacata
     1261 accacagcat ccgcactcga tacaatcgcc aattccgaag cgctcggctt cttcgagacg
     1321 attgaaatcg acgcagcctt tgatgcgaga cggcatcagg cgcatcgggc agccctcgac
     1381 gcaacgaccg cagcgaatgc acacctgcgg ctcaggtgct tcgccggctt cttcttcgtc
     1441 ccagcagagc aggcctgagg tgcccttcac gaccggaacc tgaagattga acagggcgtg
     1501 tcccatcatg gggccaccca tgatgatctt tttcggcggc cgatcgggtt tgaatccacc
     1561 tgcggcctgc acaaagtcgc cgatgagcat gcccatggga accaggtaat tgccgggttt
     1621 tgccacggca ttgcctgcca gagtcacgat gcgatcgacc accggtttct ccatgtagat
     1681 ggcatcatag atggcagcag ccgtcgccac gttctgcacg accacaccga catccatggg
     1741 cagcccgcga gacggaacct cgcgctgcag cacggcgtaa atcagctgtt tttcgccacc
     1801 ctgcggataa cagacatgca gcggagccac cctgatgtgg gattcatggc ggcatttttc
     1861 ggtgaacagg gcaatcgcat cgggtttgtt ttcttcgatg ccaatgtacc cgttcttcgc
     1921 gccgagaacc ttcatcaaca gcttcaaacc gatcagtact ttgtcgggtt gctcaagcat
     1981 caggcgatgg tcagacgtga gagccggttc acattcgact ccgttcagaa tgacggtgtc
     2041 gatcggcttc gtcggcggtg gagagacctt cacgtgggtc ggaaaggttg ccccgcccat
     2101 gcccacgagt ccggcatcct gaatacgttt tttgagttcg tcgaccggca tcagcaaccc
     2161 gtcattcttc acgcggaagg ccggcttctc agcctcggga gtctgctcga tcacgatcgc
     2221 caaagccggc gaaccgaacg ggtgcagaaa atttccgaca cccgcgatac gcccggagat
     2281 actggcgtgc accggggctg aaaccattcc ggtggcttcg gcgattttct gcccacacgt
     2341 aaccttgtcg ttgaccgcga ccaggggctt cgccggggca ccaatatgct gcgagaccgg
     2401 aagcacgacc ttgttgggcc atggcagttc aatggtggag caccctgctg tcttcttgag
     2461 ctcgggcggg tgtaccccgc ctgaaaaagc aaatgtttcc gtcatggggt gtagatgcct
     2521 ccccggggtt aggttcaggg cagagcgaaa gtagcacgat caatctgaat tttcaatgtt
     2581 ttttcgttct caaaggaggg gtagaacttc gcgaagcgat tgatctttcc ggtacccctg
     2641 tgttaagtca ttcggtggaa cagaatccgc tttttgattc gcgcgttacc agagagtgca
     2701 aaccaaccgg gaggctaccc catgattcct gtcacgaccg tgcgcgtctc gctcctgatg
     2761 ctgctttgtt gtctgctttc ctcactcgtt ctccctctgc cagcggctgc ggccaatcgc
     2821 gccgacctct ggaaaaaggt ggaaaaagca atcaatgaag gacttcccaa gaccgcgatc
     2881 gagcatctga cgcctattgt cgacgcggct ctccaggaca aagcctacgg cgaggccgca
     2941 cgggccatgt gccggaaaat tgtgctcgaa gccaacattc agggcaacaa ggccgaggag
     3001 aaagtcaccc ggctgcaaac caccctcgaa acggctcccg gcgaactcaa acctctgctg
     3061 cgagcagttt tggcgcggtg gtactggcat ttcttccgcc agaacaactg gcgtttcatg
     3121 aaccgcaccg cgacagccaa actcgacgaa aaggacttca ccacctggga tctcccgaaa
     3181 ctcttcggac acatcggcgg gttattcgac gcggcgatcg ctgacaaagc ctcactgcag
     3241 gcactttcca tcaaggagtt caacgaattt ctctccagcg ggaacatgcc ggcggagtat
     3301 cggccaaccc tgtatgattt cctcgtctat caggctctcg agttctatac ctcgggtgaa
     3361 caggccggtg cctatccgca ggatgcgttt ttcgtcgaag ccgattcgcc cgccctggct
     3421 gacgcttcag cattcatggc ctggaacccg gcaaccacag acaccgattc gccgaaactc
     3481 aaggcaattc gcctgctgca ggatctcctc aagttccacg ccaaagacaa ggatcgcggg
     3541 gcatttctcg acgccgacat ctatcgtctg cgctggttga aatcggtggc cgtcggcgaa
     3601 aacgccgccg aagcctgttt caagcagctc gaggtcatcg cccgtgagtt tgccgatcat
     3661 cagctttcga gcaccgcttt cgaagccatg gcccagatcc ggttcgccga gggcaaccgc
     3721 aaggaagcgc accagctggc ttcggaggga tccaagcgat ttccgaactc agccggcgga
     3781 cacaactgcc gctctctgat cagccagatc gagcagaagc tgtctgatcc cgaattttac
     3841 aaaaccggtg gc
//
LOCUS       DAORHD010000333         3851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_25917_length_3851_cov_3.955479, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000333 DAORHD010000000
VERSION     DAORHD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25917_length_3851_cov_3.955479"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..498
                     /locus_tag="PKO06_08260"
     CDS             49..498
                     /locus_tag="PKO06_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV69674.1"
                     /translation="MTKRDSGTRLIQIPQKDFHVRKPEVPASAPTASSSYSFIEEDRQ
                     GTLLITIHGYFNDQAGRDLSELVNRSLRGDGKRFVFDFQHCSTINSMGVAELLEITLR
                     ITEDFKGQLVLSALKPFMADLLTLAYVIPAAQVAPDIESALERLVDG"
     gene            522..3260
                     /locus_tag="PKO06_08265"
     CDS             522..3260
                     /locus_tag="PKO06_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69675.1"
                     /translation="MPTVSNSTDAGAELRRDLDSPIRAVRLFAYDEILRTRSAMRLRD
                     ALKQRETLEDDEECQILLRHALAMASASEKSDTSSAETVPAEKSGVLLSAIGSRTPEE
                     WQKMAQEFPASLAGESDPLILAELIRRIGRYLDPAQRRALEPYLVHPLSLVRSAALAV
                     LIDRFPEELAPRLPQALRSRDPRLRALAVRGLARIDPEEAIRHLEIMLLGSDPGLRRF
                     GLQIVSLLPFPLVRDLMVRFALVAEEPVLVRAASALFRSNPDPDVGFRLLEAEQQASE
                     KQKPVLRLMFQQAELALESSGILGAEAARHRQQIQQWERKLVLRASVRDLLAQIPEDA
                     PDPQHRLIDLLGVRLQEPLVRQALEESLTWSLPPHLEGAVKGVLHPPSAQAPVVAPPP
                     SAMAAAVPAPDLERRLAKLGISEKSEPGLDLVTQALTDARQTVEVQALALRAAAVWAV
                     KGFEEQAARWLVHADHRLVAGALEYLTVTDPERVYPQLGRFLASPQPLVKLRAILLLK
                     RYDLPQAISALLALIRLPDSRTHRLLATSLVQFEFSLLRGPLTDFLCQTGGGTFLRES
                     LCLFQANPARENLFCLYRIEQAVAKTHRFAVQKTRESCENLLIELGLLSRDEIVSVQA
                     QWELELQRKQARAHNPPSYAIEQVFSDLSGISVRFSAVRRAFAEIDEATVKKTIFMVF
                     VFAPLVYAWWTYFLQPTTPFSLAPGANTALQRLARTPTVGKTAAPVATRPPAPPPSTL
                     EDFSRALEREIDRELSAPLPGGMSQDAAWMFQASEHPAIGRILEKIELGRGDEAIEDL
                     ERLLVQAPDNPYLQMRVLSILCRLYQSKGDAASLRKAQERFATALDRLPGFFPVSFRE
                     FLRAQEQMLKALKQPNWQAAASQTEPVDRSQILAGISQLEAGFPGVTQVNKKDAEE"
     gene            3257..>3851
                     /locus_tag="PKO06_08270"
     CDS             3257..>3851
                     /locus_tag="PKO06_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69676.1"
                     /translation="MSRQNRRGFLLGVVIALVAVLIIGVWFLFRSTLEQGRQTLLTRD
                     GLQARCLAEAALNRALRVFQDRLNDPAAYGPSGSAENPAVKLRLPLPVADAASGRLST
                     SGIPEFVFTRADLIGTGTASSVPSLDALYAFMMDGRPGTYSIRVTARVEEAYRVLGDG
                     AQTTPVLGVDQPWNIRRDVREFLEGKGYLSFFLQFPPD"
BASE COUNT          713 a         1117 c         1179 g          842 t
ORIGIN      
        1 aacacgtaag gaggagaatt aaatgaccct gagttgcggg cgaacgtctt gacgaagcgt
       61 gacagcggca ctagactgat tcagattcca cagaaggact tccacgtgag aaaaccagag
      121 gttcctgctt ccgcgcccac ggcgagctct tcctattcct tcatcgagga agatcggcag
      181 ggcactctcc tgatcaccat tcacgggtat ttcaacgatc aggcggggcg cgatctctcg
      241 gaattggtga atcgaagtct gcgcggggat ggcaagcggt ttgtgttcga ctttcagcac
      301 tgttccacca tcaacagtat gggggtcgcg gaactgctcg aaatcacgtt gagaatcacc
      361 gaagacttca aggggcagct ggttctttcc gctctgaagc cattcatggc ggatctgctg
      421 acgctggcct atgtcattcc cgcggctcag gtcgctcctg acatcgaatc ggcccttgag
      481 cgactggtcg acggctgatc cgtggcgaga gaggtgtctc catgccgacg gtaagcaact
      541 caacagatgc aggggcggaa ctgcggcgcg atcttgactc tccgattcgc gccgttcgtc
      601 tttttgccta tgacgagatc ctgcgtaccc ggtcggccat gcgtctgcgc gatgccttga
      661 aacagcgtga aacgctcgaa gatgacgaag agtgtcaaat tctgctgcga catgcgctgg
      721 ccatggccag tgcgtctgag aaatcagaca cctcctctgc cgaaactgtg cccgcggaaa
      781 agtccggggt gctgctctcg gcaatcggtt ccaggacacc tgaagagtgg cagaagatgg
      841 cgcaggagtt cccagcctca cttgccggtg aatccgatcc gctcatactt gccgaactga
      901 ttcgccgcat cggccggtat ctcgatccgg ctcagcgtcg agcgctcgag ccgtatctcg
      961 tgcatccatt gtcgttggta cgctcggccg cgctcgccgt actcattgac cggtttcccg
     1021 aagagctggc cccacgattg ccgcaggcgc tgcgatcacg cgacccgcgt ctgcgggcat
     1081 tggcggtgcg cggcctggcc cgcatcgacc ctgaagaggc gattcggcat ctcgaaatca
     1141 tgttgttggg gtcagatcct gggttgcggc gtttcggtct gcagatcgtc tccttactgc
     1201 cgtttcccct tgtccgtgat ctgatggtgc gatttgctct ggtggcggaa gaaccggtgc
     1261 tggtgcgggc ggcatccgca ctgttccgat cgaacccgga tccggatgtc ggctttcgac
     1321 tgctggaggc agaacaacag gcttccgaga agcagaagcc ggtactgcgt ttgatgttcc
     1381 agcaggcgga actggcactc gagtcttccg gcattcttgg ggcggaagcg gctcgacatc
     1441 gccagcagat tcagcaatgg gagcggaagc tggtgttgcg cgcttcggtg cgagatctgt
     1501 tggctcagat tccggaagac gctcctgatc ctcaacacag gctgattgac ctgctggggg
     1561 tgcgcctcca agaaccgctg gtgcgtcagg ctctcgaaga gagcctcacc tggagtctcc
     1621 cgcctcacct ggaaggagcc gtcaaaggcg ttcttcatcc gccgtcggca caggcacctg
     1681 ttgtcgcgcc gccgccgtcg gccatggctg ccgctgttcc cgcgcctgat cttgaacgtc
     1741 gtctggccaa actcggcatc agtgaaaagt cggagccggg actggatctg gtgacacagg
     1801 ccttgaccga tgccagacaa acggtcgagg tgcaggcgct ggctttgcgt gccgcggccg
     1861 tctgggcggt gaaggggttt gaggaacagg ctgcaaggtg gctggtacac gcggaccatc
     1921 gtctggtggc cggggctctc gagtatctga ccgtgaccga tccggagcgc gtgtatcccc
     1981 agttgggccg ttttctggcc tcaccacagc cgttggtaaa actgcgcgcc attctcctgt
     2041 tgaaacgcta tgatctgcca caggcgattt ccgcccttct ggctctcatt cggctgcctg
     2101 actctcgtac gcaccgtttg ctggcgacgt cactggtaca attcgagttc tcgctgctac
     2161 gcgggccgct gacggacttt ttgtgtcaga ccggcggggg aaccttcctg cgagagtcac
     2221 tctgtctttt tcaggcaaac cccgcgcggg aaaacctgtt ctgtctctat cgtatcgaac
     2281 aggcggtagc caagactcac agatttgcgg tccagaaaac gcgtgaatcc tgcgaaaacc
     2341 tgctgattga actcggactc ttgtctcgag acgagatcgt atcggtccag gcccaatggg
     2401 aactcgaact gcaacggaaa caggccagag cgcacaaccc tccatcgtat gccattgagc
     2461 aggtgttctc tgatctgtcc ggcatctccg ttcgtttttc tgccgtgcgt cgagcatttg
     2521 cggagattga tgaggcgacg gtgaagaaga ccatctttat ggttttcgtg tttgctccgc
     2581 tggtctatgc ctggtggacc tactttctgc aaccgacgac cccattctcc ctggctcccg
     2641 gagccaatac cgccctgcag cgtctggcac gaaccccaac ggtgggaaaa accgctgcgc
     2701 ctgtggcgac gaggcccccg gctccgccac cgtcgaccct cgaagacttt tcgcgggcgc
     2761 tggagcgaga gatcgatcgc gaactgtctg ctcctctccc gggcgggatg agccaggatg
     2821 cggcttggat gtttcaggca tccgaacacc cggcgattgg ccgaatcctc gagaagattg
     2881 aactcggccg gggagacgaa gcgatcgaag acctcgagcg cttgctcgtt caggcccctg
     2941 acaacccgta tctgcagatg cgtgtgcttt cgattctttg tcgtttgtac cagtcgaaag
     3001 gagatgcggc gtccttgcgg aaagcgcagg aacgattcgc gacagctctt gatcgtcttc
     3061 ccggtttctt tccggtgtcc tttcgtgaat ttctgcgagc tcaggagcag atgctgaaag
     3121 cactgaagca accgaactgg caggcagccg cgagtcagac ggagccggtg gatcggtcgc
     3181 agatcctggc gggaatctcg cagctggaag caggattccc tggtgtgaca caggtgaaca
     3241 agaaggatgc tgaggaatga gcaggcagaa ccgtcgcggt tttcttctgg gcgtggtcat
     3301 tgctctggtg gccgtactga tcatcggagt ctggtttctt tttcgcagta cgctcgagca
     3361 ggggcgccag accctgctga cgcgagacgg tctccaggcg cgctgtctcg cggaggccgc
     3421 gctgaatcgg gctttgcggg tgtttcagga ccgtctgaac gacccggctg cctatggacc
     3481 ttctggctcg gctgagaacc cggccgtgaa gctgcgtctg cctctgccgg tcgcggatgc
     3541 cgcgagtggc cgactcagta cgagtgggat cccggaattt gtctttaccc gagccgatct
     3601 cattggaacg ggaaccgcgt cgtcagtccc cagccttgat gctctctacg cgttcatgat
     3661 ggatggccgc ccgggcacct acagcatacg agtgacggcg cgagtggaag aggcgtatcg
     3721 ggtgttgggc gacggggcgc agacgacccc ggttctcggc gtcgatcaac cctggaacat
     3781 tcgccgcgac gtccgcgagt ttctcgaagg gaagggatat ctcagctttt ttcttcagtt
     3841 cccgccggat a
//
LOCUS       DAORHD010000334         3834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26082_length_3834_cov_3.371791, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000334 DAORHD010000000
VERSION     DAORHD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26082_length_3834_cov_3.371791"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PKO06_08275"
     CDS             <1..1030
                     /locus_tag="PKO06_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69677.1"
                     /translation="SQMLEIVKRTPGTLDVLSSLQTGKPEVRIIPRREELARHGLNEA
                     FLALSLRASYEGDTTTKYREGDDEYDIRIKLDGRVRRDVSEVEKLVIVSPQGTAIPLS
                     ALARVEETTGPAQINRKSRTKLIQITANIAGRSLGEIVQDIRRDMAPIDLPLGYSITF
                     AGAVERMQESFASLLTSLVLAVIFTFMLLAALLESFIHPFTILLSFPLAFAGIFLGLF
                     LTGQTLSIFSLMAVVMLVGIVVNNGILLIEEYKLRRESGCTLFAAILEGSPAKLRPII
                     MTTVASVASMVPLAMGSGSGGEMRAAMATVSIGGLLVSGLLSLVVIPLVYSVVEQKLR
                     PDAVSSAG"
     gene            complement(1205..3262)
                     /locus_tag="PKO06_08280"
     CDS             complement(1205..3262)
                     /locus_tag="PKO06_08280"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HNV69678.1"
                     /translation="MTGLSLKWRLILGGVCLAILPLVIIGWYVSVQNRHMTEVARAES
                     MILGRADLDHISEGVYGSIQAQQEVLQQQVAANLNVTAELLKKLGGISLSDSKVQWEA
                     KNQFSGVVQTVSLSELKVGGTAVVRNEDPAVASPLVDQAKALMGGTCTIFQRMNDEGD
                     MLRVVTNVVTKEGKRAIGTYIPARMPDGSANAVLASVLAGKRFLGRAQVVGVWCVTAY
                     EPLYGTDGKVIGALYFGVPEESATSLRKQIMDIKVGKTGYVFVLDSKGNYVISAGGKR
                     DGENIWEAKDSDGKTMIQDMIKIALGLKNREIGEYTYNWKNPGDVTGRKKIARLMYFA
                     PWDWVIGASSYEDEFLEAATKLENVSKTINRNILGLILIVMAIAVTISLVFALSVSNT
                     LTSIAERLRTGSNEIASASGQIAETSQSLASGASEQAAGLEESTAALRDIGARSKEVG
                     ELTKGADALMAQNIQTTGQSLKSLVELTQAMIKIEADGSEMSKIIKTIDEIAFQTNLL
                     ALNAAVEAARAGEAGKGFAVVAEEVRSLAQRAAEAARSTQEKLEGNIGRVAQATKGIR
                     GINTNFESIVETATVMGEKTANMSNAVTEVTRNIDQISSAFAELDQVVQSTAASAEES
                     ASASEQMSAQAHELNGMVSELVTLIEGSAATKQQNDLFTPATKPLPPLPDEPRTATRK
                     KNT"
     gene            complement(3291..3599)
                     /locus_tag="PKO06_08285"
     CDS             complement(3291..3599)
                     /locus_tag="PKO06_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV69679.1"
                     /translation="MNISVSQTGDQAILELEGAVDERGAEDLKQRFRSLPLNQLKSVI
                     IDLGKVTHIGSAGIGKMLVFYKDLAVHDAKLSLIKVPALIYQMFVEMKLDTLFSIARA
                     "
     gene            complement(3596..>3834)
                     /locus_tag="PKO06_08290"
     CDS             complement(3596..>3834)
                     /locus_tag="PKO06_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69680.1"
                     /translation="RDPTSFDNRSKPSGRGLFVLRLVAEEVEWCENGRVVTLYFPRRT
                     WERPKAAGQVCRTNLAFWPETDESLSDTEKESCV"
BASE COUNT          745 a         1185 c         1014 g          890 t
ORIGIN      
        1 gagccagatg ctggaaatcg tcaaacgaac cccgggcacg ctcgatgttc tcagcagcct
       61 gcaaaccggc aaaccggaag tgcgcatcat cccccggcgc gaggaactgg cccggcacgg
      121 actcaacgag gcgtttctcg ccctgtctct gcgcgcctcg tatgaaggcg atacgacgac
      181 caagtatcgt gagggagatg acgagtatga cattcgcatc aaactcgatg gccgcgtgcg
      241 ccgcgacgtg tcagaagtcg agaagctggt catcgtctca ccgcagggca cggcgattcc
      301 cctgtcggcc ctggcccggg tcgaggagac aaccggaccg gcccagatca accgcaagtc
      361 tcgcaccaaa ctgattcaga tcaccgccaa catcgccggt cgcagtctcg gcgagatcgt
      421 tcaggatatc cgccgcgaca tggccccgat cgatctgccg ctcgggtaca gcatcacctt
      481 cgcgggggcg gtcgaacgca tgcaggaatc gtttgccagc ctgctgacct cactcgtgct
      541 ggcggtcatc ttcaccttca tgctgctggc cgccctgctc gaatccttca ttcacccgtt
      601 cacgattctg ttgagctttc cgctggcgtt tgccggcatc tttcttggac tcttcctgac
      661 cgggcagacc ctgagtatct tctcgctcat ggccgtggtc atgctggttg gaatcgtcgt
      721 caacaatggc attctgctca tcgaagagta caagctgaga cgtgagagcg gctgcacgct
      781 cttcgcagcc atactcgaag gctctcccgc caagctgcga ccgatcatca tgaccacggt
      841 cgcctcggtg gcctcgatgg tcccgttggc gatgggcagt ggatcaggag gggaaatgcg
      901 cgcggcgatg gccaccgtct cgatcggcgg cctgctggtc tccggcctcc tctcgctggt
      961 ggtcatcccg ctggtgtatt ccgtggtaga gcagaaattg cgcccagatg ccgtatcctc
     1021 agctggttag cctgttcatc gtcttcgatc gcctgcgttc agacagtggc ggaattctga
     1081 atttgtccag tttgatacca cagcgaaaca ggccgatgat cgatatgtaa gatcccttga
     1141 actgcgaagc cccggcagag ttatctctgc cggggcttct tctgtttcga tatctcgcgc
     1201 gatgctacgt attcttcttc cgtgttgccg ttcggggctc atctggcaaa ggcggcagcg
     1261 gttttgtcgc aggggtgaac agatcattct gctgcttcgt tgccgcagag ccttcaatca
     1321 gggtaaccaa ctcggacacc atgccattga gttcgtgcgc ctgagccgac atctgttcgg
     1381 aggcactggc cgactcttcg gcgctggcag ccgtgctttg cacgacctga tcgagctcag
     1441 cgaatgccga cgagatctga tcgatgtttc tcgtgacttc ggtcacggcg ttcgacatat
     1501 tggccgtttt ctcacccatg acggtcgccg tctcgacgat cgattcgaag ttcgtgttga
     1561 ttccgcgaat gcccttcgtc gcctgggcta ctcgaccaat gttgccttcg agtttttcct
     1621 gcgtgctgcg agcggcttca gcagcgcgtt gtgcgagtga gcgcacctct tcggccacga
     1681 cggcgaaacc cttgcccgct tctccggccc gggcggcctc aaccgccgcg ttgagcgcca
     1741 gcaagttggt ctggaaggcg atttcatcga tggtcttgat gatcttcgac atctcgctgc
     1801 cgtcggcttc gatcttgatc atggcctgcg tcaactcgac cagcgacttg agcgactgcc
     1861 cggtggtctg gatgttctga gccatcagcg cgtcagcgcc tttggtcaac tcgccaacct
     1921 ctttcgaacg cgcgccgatg tctcgcaaag ccgcggttga ctcttcgagg cccgccgcct
     1981 gctcgctagc gccgctggcc agagactgac tggtctcggc aatctgaccc gacgctgatg
     2041 cgatctcgtt tgaaccggta cgcaaccgtt cggcaatcga cgtcaaggta ttcgaaacgc
     2101 tgagagcgaa caccagactg atggtcacgg caatcgccat cacgatcaga atcagcccca
     2161 gaatgttgcg attgatcgtc ttcgagacgt tttcgagctt ggtcgccgct tccagaaact
     2221 catcctcata ggaactcgcg ccgatcaccc agtcccatgg ggcaaagtac atcagacggg
     2281 cgattttctt ccgcccggtc acgtcacccg ggtttttcca gttgtaggtg tattcgccaa
     2341 tttcgcggtt cttgagcccg agcgcgatct tgatcatatc ctgaatcatg gtctttccgt
     2401 ccgagtcttt tgcttcccag atattctcgc catctcgctt tccaccggca gaaatgacat
     2461 agttgccttt ggaatcgaga acaaagacgt agccggtctt tccgaccttg atatccatga
     2521 tctgcttgcg caggctggtg gcgctctctt cgggaacccc gaagtagagg gctccgatca
     2581 ccttgccatc cgtaccatac agtggttcat atgccgtcac gcaccagaca ccgaccacct
     2641 gagcccgccc gagaaagcgt ttgccagcca acacgctggc taaaacagca tttgcggagc
     2701 catccggcat cctggccgga atgtaggtgc cgatcgcccg tttcccttct ttcgtcacga
     2761 cgttggtcac gacccgcagc atgtcgccct cgtcgttcat tcgctgaaag atcgtgcagg
     2821 tgcctcccat gagggctttc gcctgatcaa ccagcggcga agccaccgcc ggatcttcat
     2881 tgcgaaccac cgcggttccg ccaaccttca gctcagacag cgaaaccgtc tgtacaactc
     2941 cagaaaactg gtttttcgct tcccattgaa cttttgaatc acttagactg attccgccga
     3001 gttttttcaa cagttcggcg gtgacattca agttcgccgc aacctgttgc tgcagcacct
     3061 cctgctgtgc ctggatcgac ccgtagacgc cctcggagat gtgatcgaga tcagctcgac
     3121 cgagaatcat gctttcggcg cgagcgactt ccgtcatatg tcggttctgc acgctgacat
     3181 accagccgat gatcaccagc ggtagaatgg ccaggcacac accgccaaga atcaaacgcc
     3241 acttgagtga tagtccggtc atttgtccct ccctgaaata gcggatcgag ttaagcccga
     3301 gcgatggaaa acagcgtatc gagtttcatc tcgacaaaca tctggtagat caacgccggg
     3361 actttgatga gactcaactt ggcatcatgc accgccagat ccttgtaaaa aacgagcatc
     3421 ttgccgattc cggcgctgcc gatgtgtgtg acttttccga gatcgatgat caccgacttc
     3481 agctgattca gcggcaagct gcgaaaacgc tgtttcaaat cttcagcccc gcgctcgtca
     3541 accgctcctt cgagttcgag aatggcctga tcaccggttt gactcacact gatgttcata
     3601 cgcaagactc cttttccgtg tctgacagac tttcatccgt ttctggccag aaagccagat
     3661 tcgtcctgca cacctgcccc gccgcttttg gtcgttccca ggtgcggcgc ggaaaataca
     3721 gggttactac tcttccattc tcgcaccact cgacttcttc tgcaaccagg cgcaggacga
     3781 acaatccacg ccccgatggc ttgctgcgat tgtcgaaaga ggtcggatcc cgta
//
LOCUS       DAORHD010000335         3828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26127_length_3828_cov_3.443149, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000335 DAORHD010000000
VERSION     DAORHD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26127_length_3828_cov_3.443149"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="PKO06_08295"
     CDS             complement(<1..1309)
                     /locus_tag="PKO06_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV69681.1"
                     /translation="MTTQTKQRKRLGDTLVEAGLITADQLKDALARQKAVGLRLGRVL
                     VELGMANEESIARTLARQMNIPFLNLNETTIQPEVLAIIPENIARSHQLLPIQLEGNR
                     LRVSMADPLDVFIIDEINYQTGFEIEVAISPESQIEAATRQYYGSSDTLKQAVDTLAA
                     EKRDDRVQLDEAFFTTFELGSDDAAVPMINLVNTIIQQAISDKASDIHIEPDEDLIRI
                     RYRVDGILTEIMKAPKNIQSELLSRLKIMAQMDISEKRLPQDGRIKVKVKDTGIDLRV
                     SSLPTVFGEKIVIRILDKSRLQLTLEQVGFDDELLTLFKTLSKSPNGIILMTGPTGSG
                     KTSTLYAALNFVRNGQINITTVEDPVEYQIPSINQVQVRPDIGLTFARALRSILRQDP
                     NVVLIGEIRDFETAQIAIEAALTGHLVFSTLHTNDAASAVTRLV"
     gene            complement(1306..2061)
                     /locus_tag="PKO06_08300"
     CDS             complement(1306..2061)
                     /locus_tag="PKO06_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69682.1"
                     /translation="MVIEKNPSAYFSTGLLEELCEENRRKLAADPENDSLHFNLANAL
                     SRLGKFDEAVTEYQAAIQRQPKPVYLNNLGKTLNDMGNYEEATRIFDQVLQLTKWPDT
                     WYHKALAFKQLGRLEEADQALLEALKLNPQYREAINARGEVLEALNKPVEALFEYKKV
                     IALFFSDDQTRESEDFDYDLSILYSTTEMVDESIRQLERFVKKNPGFADAHYKLGLAY
                     QAAGREHEAMLAFRRALEINPRYETARQSFWKR"
     gene            complement(2069..2878)
                     /locus_tag="PKO06_08305"
     CDS             complement(2069..2878)
                     /locus_tag="PKO06_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV69683.1"
                     /translation="MSTTRKFCWLWACAFAGSLILASSDPSSAFNLFKKKKKKAQQTT
                     DKAADSKTPAKPLRKIPAVTSHHFFAFEDAPYYTSADKRIGVKMVVDAVKVGPTMAAT
                     QHITFLPGASMPSHRHVYVTEVVYVLKGNLTLRIDKETKVMGPDSCAYIPPQTFHEYA
                     NTSDDVCQWLQIYSPAGPEEEYRNWETPDTPKVTATEKPVATGTQHIVAPPRQPLPGS
                     PIPHLDTVKESDVPTTEAASQTLQLKAPAAASMTLQLKKADPTAKPIKKAN"
     gene            3037..3561
                     /locus_tag="PKO06_08310"
     CDS             3037..3561
                     /locus_tag="PKO06_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69684.1"
                     /translation="MLLLLVTTMFTGCWGRAKVGTPDRKPVVVASEAVLVRETPASTT
                     SSIASASRVASQSAPADRARQQELELEANLEIVSKMIRTANLDAALRRIEKTEQENPG
                     NAHLLMKTGYLKAMIFHRQKDSVKRRDAMNQMLKNMETMQKDPMYRTAFTDGQDAIDV
                     VRKSLERGGGRYGP"
     gene            3551..>3828
                     /locus_tag="PKO06_08315"
     CDS             3551..>3828
                     /locus_tag="PKO06_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69685.1"
                     /translation="MVHNRRGSIFLLALGALVILFILGIALTFFTGSEDWSSGLSYES
                     EVAFNLAESATEEFVARLKNALNHDDPGNMLFKVLRGKNIDPDKEIPL"
BASE COUNT          784 a         1015 c         1132 g          897 t
ORIGIN      
        1 cgacgagtcg tgtgaccgcc gaggccgcgt cgttggtatg cagtgtcgaa aagacgagat
       61 gaccggtgag agctgcctca atggcgatct gggccgtctc aaagtcgcgg atttccccga
      121 tcagcaccac gtttggatcc tgacgcagga tcgagcgcag ggctctcgca aacgtcagcc
      181 cgatatcggg ccgcacctgc acctgattga tcgatggaat ctggtattcg accgggtctt
      241 caaccgtggt gatgttgatc tgcccattac ggacgaagtt cagggcggca tacagcgtcg
      301 aggtcttgcc tgagccggtc gggccggtca tcaggatgat gccgttcgga gacttggaga
      361 gcgttttgaa cagggtcaga agctcgtcat cgaacccgac ctgctcgagg gtcagctgca
      421 ggcggctctt gtcgagaatg cgaatgacga ttttttcgcc gaaaaccgtt ggcagagagg
      481 aaacgcgcag atcgatgccg gtatctttca ccttgacctt gatgcggcca tcctgcggca
      541 ggcgcttttc cgaaatatcc atctgggcca tgatcttcag gcgcgacagc agctcgctct
      601 gaatattctt cggagctttc atgatttcgg tcagaatgcc gtcgacacgg tagcgaatgc
      661 ggatcaggtc ttcgtcgggt tcgatgtgaa tgtctgaggc cttgtcagaa atcgcctgct
      721 gaatgatggt attgaccagg ttgatcatcg gcacggcggc atcatcgctg cccagttcga
      781 aggtcgtgaa gaacgcttcg tcgagctgaa cccgatcatc acgtttctcg gctgccaggg
      841 tgtctaccgc ctgtttcagc gtgtcactgc tgccatagta ctggcgggtt gctgcttcaa
      901 tctgcgattc cgggctgatc gccacctcga tctcaaaacc ggtctgatag ttgatctcat
      961 cgatgatgaa gacgtcgagc gggtcagcca tgctgacgcg caaccggttg ccctcgagtt
     1021 gtatcggcag cagctggtgg ctgcgagcga tgttctccgg aataatggcc agaacctcag
     1081 gctgaatcgt cgtttcgttg aggttgagga agggaatgtt catctgccgc gccagagtgc
     1141 gggcgatact ttcctcattg gccatgccga gttcgacgag aacgcggccc aggcgaagcc
     1201 caaccgcctt ctgtcgcgcg agcgcgtctt tcagctgatc agccgtgatc aaccccgctt
     1261 cgacgagggt atcgccgagg cgcttgcgtt gtttggtttg cgtggtcaac gtttccagaa
     1321 ggactgccgc gcggtttcat accgcggatt gatttcgagc gcacgtcgaa aagccagcat
     1381 ggcttcgtgt tcgcgcccgg cggcctgata ggcgagtccg agtttatagt gggcgtccgc
     1441 aaagcccggg ttctttttca caaatcgttc gagctggcga atcgattcat cgaccatctc
     1501 cgtggtcgaa tacagaatcg acaggtcgta atcgaaatct tctgattctc gcgtctggtc
     1561 gtccgagaag aacagcgcga tcactttctt atactcgaac aatgcctcaa ccggcttgtt
     1621 cagggcttcg agcacttctc cgcgggcgtt gatggcttcg cgatactgag ggttcagctt
     1681 gagggcttcc aaaagggcct ggtccgcttc ttccaggcgg cccagctgct tgaatgccag
     1741 ggccttgtga taccaggtgt cgggccattt cgtcagctgc agaacctgat cgaagatgcg
     1801 cgtggcttct tcgtagttgc ccatgtcatt gagggttttc ccgagattgt tcaaatacac
     1861 gggctttggc tggcgttgaa tggctgcctg atactccgtc acggcttcgt cgaacttgcc
     1921 cagtcgcgac agagcgttcg caaggttgaa gtgcagactg tcgttctcgg gatcagcggc
     1981 cagtttgcgg cgattttcct cgcagagctc ttcgaggagc ccggtactga aataggccga
     2041 cgggtttttc tcgatgacca tccgaatctc agttcgcctt cttgatcggc tttgccgtcg
     2101 gatcagcctt tttcaactgc agggtcatgg aagcggccgc gggagccttg agctgaagcg
     2161 tctgtgaagc ggcttccgtc gtcgggacgt cactttcctt caccgtatcg aggtggggaa
     2221 tcggagaccc gggcagaggc tgtcgcggcg gagccacgat atgttgcgta cctgtcgcaa
     2281 ccggtttctc tgtcgcggta accttgggcg tgtctggcgt ttcccagttg cggtattctt
     2341 cttcgggacc cgcaggagaa tagatttgca gccactgaca cacatcgtca gaggtgttgg
     2401 catattcgtg aaacgtttgc gggggaatgt aggcgcagga atccggcccc atcaccttgg
     2461 tttccttgtc gatgcgcagc gtcaggttgc ccttgagcac atacacgact tctgtgacgt
     2521 agacatgacg atgcgaaggc atggaagcac cgggcagaaa agtgatgtgc tgcgtggcgg
     2581 ccatggttgg cccgactttc accgcgtcga cgaccatctt gacgccgatg cgcttgtctg
     2641 ccgaggtgta gtagggcgca tcttcgaacg caaaaaagtg gtgcgaggtg accgccggga
     2701 tcttgcgcaa cggcttggcc ggagtcttgg aatcagcggc cttatcagtg gtctgctggg
     2761 cttttttctt ctttttcttg aagaggttga aagccgacga cgggtcagaa gaggccagaa
     2821 tcaacgaacc ggcaaaggca caggcccaaa gccagcaaaa ctttcgtgtt gttgacaacg
     2881 tagtattcct ttgaggagga gatggcgaat tttatccgca aaggtgtacg cctgtcaagg
     2941 cggattccat tgactttgcc tctctgatcc agtaccatag actggatgaa aaggcctctt
     3001 tccggtgaca gagccaacag atcggcagag ctgacggtgc ttctgttgct cgtcacgacg
     3061 atgttcacgg ggtgctgggg gcgggcaaag gtcggaacac ctgatcggaa gcccgtcgtc
     3121 gttgcttctg aagccgtgct ggttcgtgag acgcccgcca gcaccacaag ttcgattgct
     3181 tctgcctctc gtgtggcgag ccagtcggca ccggctgatc gggccagaca gcaggaactc
     3241 gaactcgaag cgaacctgga gatagtttcg aaaatgattc gaaccgcgaa tctcgacgct
     3301 gccctgaggc gcatcgagaa aacagaacag gaaaacccgg gcaacgctca tctgttgatg
     3361 aaaaccgggt atctgaaggc aatgatcttt catcgacaga aagactcggt caaacgtcgg
     3421 gatgccatga accagatgct gaaaaacatg gaaaccatgc agaaagatcc gatgtatcgc
     3481 acggccttca ctgacggcca ggatgccatt gatgtcgttc gcaagtcgct cgaacgcgga
     3541 ggtggacgat atggtccata atcgacgcgg atccattttc ctgctggcgc tcggtgcgct
     3601 ggtcattctc ttcattctcg gaattgccct gacctttttc accggttccg aagactggtc
     3661 gtcgggcctt tcctacgaat ccgaggtggc gttcaatctc gccgaatcag ccacggaaga
     3721 atttgtcgca cgactgaaaa acgctctgaa tcatgatgac ccgggaaaca tgctgttcaa
     3781 ggtattgcgc ggcaagaaca ttgatcccga caaggaaatt cctctcga
//
LOCUS       DAORHD010000336         3804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26381_length_3804_cov_3.239264, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000336 DAORHD010000000
VERSION     DAORHD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26381_length_3804_cov_3.239264"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2572)
                     /locus_tag="PKO06_08320"
     CDS             complement(<1..2572)
                     /locus_tag="PKO06_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV69686.1"
                     /translation="MSVFRVARSIALLLFCLFSCPSWNNPLLAQTEVVPPPIPAEVTL
                     GPTEEKSFSFFHPGGVFRGLKISPASGTLVDCRMSGRFTQGAFDLDAQAERQVEGTAG
                     PFSLFDGIPQHPGTYTLTLTNATDSATRLRIETIDLGPLPRIEPDGRSGTLIVKKAAT
                     SGIRVRPESPGVSAWGENGFSTDTPKGDLTPAGDVVMSVPAGLWRLAGTIGSLGIDIE
                     SHFVPILPGKTTIVENWPVLDQKELKGAESTVGGLRLRNVGMDGEHVKVRFSTPDLKG
                     TVDKKDLEVFEGGMPGEVLSVKRAEEPLQLVVLLDSSGSMKNDLKKVITAAEVFFRNL
                     PKGTGVNLIDFDTQPKLLKTSTAEDALKALKGFKADGATALNDSVILGLKHCHGDRSA
                     LVLFTDGFDANHNDTGPGSKAAPEEMFEVVKQAGIPVFTIGYGKKPDESTLRRLATLS
                     GGAYFRAQADTVQKVFDQLNGILGRDFELVYRRPGITAPSDVPVVCVMLDRSGSMNMS
                     PIEEGCGYRMEEAKGILRRFIEVLPDNAVVQIADFHQYITIQQAWTTNRQAMLRGISQ
                     IFSYGGTEIVGALDVAFRSLRQMPSRRRYLLFITDLGLPGLDDEPFRELAGMVKDAGI
                     HCTWVGMVDPSKRAPFDEVASFTGGTAAVANDPTSLREAAERLGKQINADKTVDPRLE
                     VRLTLARKDETGNPLVMSAADLFPLPPSPQTATASVHGIRYVITDEARQNTAGLWKAG
                     HTTELTAEAANKAFRIRALELKVLDEIGGMKPPSDERFIALRLRFECSSPTALIDNLR
                     AHLFLKWNENPETGASPLTWLGEDPLVQPDNEGLALRSGNPVEGHVVFRVPDEGASQV
                     G"
     gene            complement(2644..3585)
                     /locus_tag="PKO06_08325"
     CDS             complement(2644..3585)
                     /locus_tag="PKO06_08325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HNV69687.1"
                     /translation="MFLCLFLNSALQAEWKQTEGGGSFKNLLYVNGVLLSGNAATIQR
                     STDFGASWEEYNTELPEDGHIRDYFVTDGTYLFNMFYGFTYDVLYRSDDLGRTWKQIL
                     RKPKETLGRVHGLNGMVVILDENGAGLHVSTDGGSTWTFQKGIVVDGKTVKTNLSYIV
                     QLQNAFYAAQKREGPILVSTDGLKTFKSLSGPKDKEMSLFQAGPFLVVGEGYFDGSAN
                     RFRIHTSENGGKSWVTGSVSPTPEINFLESVVEHKGTLYCTVKGKVCRSRDKGKTWED
                     ISENLKPHMIEMGCRLALRGQELGLQACIHFYTRMAD"
BASE COUNT          746 a         1083 c         1152 g          823 t
ORIGIN      
        1 atccgacctg ggatgctccc tcgtcgggga cccgaaacac gacatgccct tccaccgggt
       61 ttcccgagcg cagggccagg ccttcgttgt cgggctgcac cagcgggtct tcgcccagcc
      121 aggtcagagg ggaagcgccc gtttccgggt tctcgttcca tttcaggaac aggtgcgcgc
      181 gcaggttgtc gatcagcgcg gtgggcgacg agcattcgaa ccgcagacgc agggcgatga
      241 atcgctcgtc cgacggtggt ttcatgccgc cgatctcgtc gagaaccttc agctcaagag
      301 cacggatccg gaaagccttg ttcgcggctt cggctgtcag ttccgtcgta tgtccggctt
      361 tccagaggcc tgccgtgttc tggcgggctt catcggtgat gacgtagcgg ataccgtgaa
      421 cggaagcagt agcggtctgc ggtgacggcg gtagcgggaa gaggtccgcg gcactcatga
      481 ccagcggatt cccggtttcg tccttccggg cgagcgtgag acggacttcc agtctcgggt
      541 ctaccgtttt gtcggcgttg atctgcttgc cgaggcgttc agcggcctcg cgcagactgg
      601 tcggatcgtt ggctaccgcg gcagttccgc cggtgaatga cgcgacctcg tcgaacggag
      661 cccgtttcga ggggtcgacc atgccgaccc aggtgcagtg aatgccggcg tccttgacca
      721 ttcctgccag ttcgcgaaag ggttcgtcgt cgagacccgg caaaccgagg tcggtgatga
      781 acagcaggta gcgccggcgc gatggcatct gtctcaaact gcggaacgcg acgtcgaggg
      841 cccccacgat ttcagtgccg ccgtaggaaa aaatctgcga gatgccgcgc agcatggcct
      901 gccgattggt ggtccaggcc tgctgaatcg tgatgtactg gtgaaaatcg gcgatctgta
      961 ccaccgcatt atccggaaga acttcgatga agcggcgcag aatgcccttg gcttcttcca
     1021 tgcgataccc acagccctct tcgatcggcg acatgttcat cgaaccgctg cggtcgagca
     1081 tgacgcagac gacggggacg tcgctgggag ctgtgatgcc gggtcgccgg tagaccagtt
     1141 cgaaatctcg cccgagaatg ccgttcaact ggtcgaacac tttctgcacc gtgtctgcct
     1201 gcgcacgaaa gtaggctccg cctgagagcg tcgccaggcg gcgcagtgtc gattcgtccg
     1261 gcttcttccc gtagccgatg gtgaataccg gaatcccggc ttgcttcacc acttcgaaca
     1321 tctcttcagg cgcagctttg ctgccgggtc cggtgtcgtt gtgattcgca tcgaaaccgt
     1381 cggtgaaaag caccagagcc gaacgatccc cgtgacaatg cttcagtccg agaatcacgc
     1441 tgtcgttgag ggcggtggcg ccatcggcct tgaagccctt cagcgctttc agcgcatctt
     1501 cggcggttga ggttttcagg agtttgggct gcgtgtcgaa gtcgatgagg ttgacgccgg
     1561 tccctttggg caggttgcga aagaacacct cggcggcggt gatcaccttc ttcagatcgt
     1621 ttttcatcga ccccgaggag tcgagaagca cgaccagttg caacggctct tccgcgcgct
     1681 tgacagacag aacctcgccg ggcatgccgc cctcgaagac ttccaggtcc ttcttgtcga
     1741 cagttccctt caggtcaggt gtcgagaagc ggactttcac gtgttcgccg tccataccga
     1801 cgttgcggag tcggagcccg ccgacagtgc tctcagcgcc tttcaactct ttctggtcca
     1861 ggaccggcca gttttcgacg atcgttgttt ttcccggcag aatgggcaca aagtggctct
     1921 cgatatcgat gccgagactg ccaatcgttc cggccagacg ccagagacca gccggaacgc
     1981 tcatgacgac gtctccagcg ggagtcagat cgcccttcgg ggtgtccgtg gaaaatccgt
     2041 tttctcccca ggcggagacg ccgggagatt cagggcgcac gcggatgccg gacgtcgcgg
     2101 ccttcttgac gatcagggtt ccggatcgac catccggttc gatgcgcggc agggggccga
     2161 gatcgattgt ctcgattctc agtcgggttg ccgaatcggt ggcattcgtg agggtgagcg
     2221 tgtaggttcc cggatgctgg ggaattccat cgaacaggga aaaggggccg gcggtgccct
     2281 cgacctgacg ttctgcctga gcatcaagat cgaatgcgcc ctgagtgaac cgtccgctca
     2341 ttcggcaatc aacgagggtg ccagaggcgg gtgagatctt caggccgcgg aaaacgccgc
     2401 cgggatgaaa gaacgaaaag gacttttctt cggttggtcc gagggtgact tctgcaggta
     2461 ttggtggcgg aacaacctct gtctgagcca ggagtgggtt attccaactc ggacaggaga
     2521 acaggcagaa aagcaacaag gcgatacttc gggcgacgcg aaaaacggac atgtggaact
     2581 cctcccacga acgattcaat gaactttcgt gcgcttgctt ccttgtctga gaaggaagca
     2641 ggatcagtcg gccatacggg tatagaagtg aatgcaggct tgaagtccca gttcctgacc
     2701 acgtagagcc agtcgacatc ccatttcaat catgtgaggt ttcagatttt cggagatgtc
     2761 ctcccaggtt ttccctttgt ctctggaacg acagaccttt cccttcacgg tgcaatagag
     2821 agttcctttg tgttcgacga cgctctccag aaaattgatc tcgggggtcg ggctgacgga
     2881 gccggtgacc cacgattttc cgccgttctc cgacgtatga atgcgaaagc gattcgcgga
     2941 gccgtcgaaa taaccttcgc cgacgacgag aaacggcccg gcttgaaaca ggctcatctc
     3001 tttgtctttc ggcccggaaa gagacttgaa cgttttcagc ccgtccgttg acacgaggat
     3061 cgggccctcg cgtttttgag ctgcatagaa ggcattctgc agttgaacga tgtaggaaag
     3121 gttggttttg acggtttttc catcgacgac gatccccttc tggaaagtcc aggtgcttcc
     3181 gccgtcggtc gagacatgca atccggcacc gttctcgtca aggatgacga ccatcccgtt
     3241 cagtccgtgc acgcgaccga gcgtctcctt cggcttccgg agaatctgtt tccaggtgcg
     3301 tcccaggtcg tctgaccggt agaggacatc atacgtgaat ccgtagaaca tgttgaacag
     3361 atacgtgccg tcggtgacga agtagtcgcg aatgtgtccg tcttccggaa gctcggtgtt
     3421 gtattcttcc caggatgccc caaaatcagt gcttcgctgg atggtcgccg cgttgccgga
     3481 aagcagaacg ccattcacat acagcagatt cttgaacgaa ccgccgcctt cggtctgctt
     3541 ccactcggcc tgcagtgcgg aattcaggaa taggcacaaa aacacgaacg acaaaacgat
     3601 gctcctctgt ttcaagggaa aagaacctcc gattttctat ttggtgttga tgttgcttct
     3661 cgtgagcccg gcctgaaaca ctacatcgag cggcggtact ggccgccgac ggcgtagagc
     3721 gcggccgaga tctggccaag ggtggcgtga cgcaccgtct gcatcatctc ggcgaagatg
     3781 ttgccgcccg agagtacgac ttcc
//
LOCUS       DAORHD010000337         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26417_length_3801_cov_4.075814, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000337 DAORHD010000000
VERSION     DAORHD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26417_length_3801_cov_4.075814"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="PKO06_08330"
     CDS             <1..1042
                     /locus_tag="PKO06_08330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV69688.1"
                     /translation="MMLVSAVKGSFLDGYVLMMARPEESIRGPSRTILYRAAGASFLM
                     LLLTCITGLLTSRMLRRPLTDLGIGLSALKNRDFSARVHPGEIRELAVVAERFNQLLD
                     NLQDLEMARSVQETLWPAEGLSGPGWRVEGRCVTAAALGGDFYDWFLLADGRLVVAVG
                     DVAGHGIPSALVAAAAKVELAMNTEFERDPATILAAINAAFMKQVGRKRPMTFWLGLF
                     DPLTSQLQYANAGHNLPLALVAGSAPRMLEGKGYPLGSRQKTAYVSGTIDLSRGGRIY
                     AYSDGLIEAQHAAKGPFDYSRLEAGAVRTMSAGAPDAIASLFQEVQEWSGQSVPDDDQ
                     TLVILQVEARND"
     gene            1035..>3801
                     /locus_tag="PKO06_08335"
     CDS             1035..>3801
                     /locus_tag="PKO06_08335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69689.1"
                     /translation="MTDRWLIPTLLTFLILVLAAVMSAESAAVQQQRLAIERTRRRLD
                     AIAGGIEVRAVGKALVRSLFTRVRGRSASDAEVVSLVARELRDIASGAIEVHLTDRDE
                     NPLWSSNPRSELFRLVRARLGSEKLPALGLRVPAWRLHRLSARPNIVAVWRLDRRIKQ
                     PGIAGVLYLIDKRLLHPQAQVSLLLSRWRDRGERFGYWNRVTGGSSHLPPEIAAAEMS
                     ALLQRFLRQQRSSVPVGEDQAVLVPMSGTGVLVGVARKPPLRIPDWLHALVVMLGFGW
                     FGRQRATGGSTLSLRWFVGLSLLVAAGLPLVLTWVFWSLFEENRTRSLEKEQFARLEQ
                     HLINLDRSLPEVIRRREKILSAVCDRLERSLDHLDPHIRDLNRLEIENQVFESGFFVS
                     SEGVHLRGFGFQETSLRQTALLDRDDRRRRIQEFLENGLFTPVKWLKAALEQDLEPES
                     VRRIWDCIISRAHQTKMESGISVLGKVIVQRYNQEVAGVETQSPSGGKQDLVYGAMID
                     SQTGDMIQGAIANFRRAIALGTGEISSFVFVDVLKDAVKRGQYFLLFFMDLRSIQGHF
                     FNELFLKPETWPEDIEFYTDSWYCPTLFPRQGRPEKLRRFLERLQPPRRLLSETTWLN
                     GRKVLLSALAGKNIRHFAVFAVTPWKVIEVQQNRLRHEMSIIAMMMALLMAAISWRLY
                     QSIIAPAERLMEGIRAIEDKRFDHRIPLVTGDEWDELAESFNQTIGSLEELEVAKVVQ
                     TQILPQETIRVSEAEFVGRSLMTGQVGGDYFDAIPNEDGSLSFVIGDVTGHGVSAALV
                     VAMVKSAFHFIHRRGVRAPAEMLKQMNAMIIRSLSKKKALSMQIGWYQPGGKLVLANA
                     GHPYPYLLLPHAEVKPLTARGMVLGVTARPRFEEVTVPLDSAGGSLVLFTDGIVEQMN
                     PRGARVSL"
BASE COUNT          683 a         1053 c         1218 g          847 t
ORIGIN      
        1 gatgatgctg gtttctgccg tgaaaggcag ctttctcgat ggctacgtgc tgatgatggc
       61 gcgtccggag gaatcgatca ggggtccgtc acggaccatc ttgtaccgag ccgctggcgc
      121 ctctttcctg atgctgctgc tgacctgcat cactggtctg ctgacctcgc gcatgttgcg
      181 gcggccgttg accgatctcg gcatcggtct ctccgccttg aagaaccggg acttttcggc
      241 tcgcgtacat cccggtgaga tacgcgagtt agctgtggtt gccgaacgct tcaaccagct
      301 gctcgacaat cttcaggatc tggagatggc gcgttccgtg caggagaccc tctggcccgc
      361 cgaaggcttg tcggggccgg ggtggcgggt tgaaggccgt tgcgtgacgg cagccgcgct
      421 gggcggtgat ttctatgact ggtttctttt ggctgatgga cgtctggtgg tcgcggtcgg
      481 cgatgtggct ggtcacggta ttccgtcagc cctggtcgcg gccgccgcca aggtcgaact
      541 ggccatgaac accgaatttg aacgcgatcc ggcgaccatt ctcgccgcga tcaatgccgc
      601 cttcatgaaa caggtcggtc gcaagcgccc catgaccttc tggctcggtc tgttcgaccc
      661 cttgaccagt cagctgcagt atgccaacgc cgggcacaat ttgcccctgg cactggttgc
      721 gggctctgcg ccgcggatgc ttgaaggcaa aggatatccg ctgggcagtc gtcagaaaac
      781 cgcctatgtg tcaggcacta ttgatctctc gcggggcggg cgcatctatg cctactccga
      841 cggcttgatc gaagctcagc atgccgcgaa ggggccattc gactatagtc gtctcgaggc
      901 cggagcggtg cgcaccatga gcgccggcgc tcctgatgcg atcgcctcgc tcttccagga
      961 agtgcaggaa tggtcagggc agtccgtgcc ggatgatgat cagacgctgg tgatactgca
     1021 ggtggaggcc cggaatgact gaccgctggt tgattcccac tctgctgacc tttctgattc
     1081 tggtgctggc agcagtgatg agcgccgagt ctgctgccgt tcagcaacaa cggctggcga
     1141 tcgaaaggac cagacgcaga ctcgatgcga ttgccggcgg gatcgaggtg cgcgcggtcg
     1201 gcaaagcgct cgtacgatca cttttcactc gagtgagggg gcgctcggcg agcgacgcgg
     1261 aagtggtgtc cctggtggct cgtgaactgc gcgacattgc atcaggggct attgaggtgc
     1321 acctgaccga ccgcgatgaa aatccgctct ggtcttcgaa cccgagatca gaactgtttc
     1381 ggttggttcg cgcgcgcctg ggctcagaga aactcccagc gctgggtctg agggttccgg
     1441 cctggcgtct gcatcgtctg tccgcccgcc cgaacatcgt ggcggtctgg cgcctggatc
     1501 gacggatcaa gcagccgggc attgcggggg ttctgtatct tatcgacaaa cgtctgctgc
     1561 acccgcaggc gcaggtctcg ctgttgctgt cgcggtggcg agatcgggga gagcgattcg
     1621 gatactggaa tcgcgttacc ggaggttcgt ctcatctgcc gcccgagatt gctgctgccg
     1681 agatgtccgc cttgttgcag cgctttctgc gtcagcaaag gtcatcggtg cccgtcggcg
     1741 aagatcaggc ggttctggtg ccgatgtccg gaacgggggt gctggtcggt gtggcacgca
     1801 aaccgcctct acggattcct gactggctgc acgcgttggt cgtgatgctg ggattcgggt
     1861 ggttcggccg gcagcgcgcg acaggcggca gtaccctttc gcttcgctgg tttgtcgggc
     1921 tcagtttgct ggtggctgcg ggattgccgt tggttctgac ctgggtgttc tggtcgctgt
     1981 tcgaggaaaa ccgcacgcgc agcctcgaga aggagcagtt tgcgcgactt gaacagcatc
     2041 tgatcaatct tgaccgctcc ctgccggaag tgatcaggcg ccgcgagaag attctcagcg
     2101 ccgtgtgcga tcgcctcgag cgttcgctcg atcatctcga cccccatatc cgcgacctca
     2161 atcgcctgga aatcgagaat caggtgttcg agagcgggtt ctttgtcagc tctgagggtg
     2221 tccatctgcg tgggttcggc tttcaggaaa cctccctgcg tcagacggcc ctgctcgatc
     2281 gcgacgatcg gcgccggcgc attcaggagt ttctcgaaaa cggtctgttt accccggtga
     2341 agtggttgaa ggccgctctc gaacaagacc tggagccgga atcagtgcgt cgtatctggg
     2401 actgtatcat ttcgcgcgcc catcagacca agatggaatc tggtatttcg gttctgggaa
     2461 aggtaatcgt gcaacggtac aaccaggaag tcgctggtgt cgagacacag tcaccatctg
     2521 ggggcaagca ggatctcgtc tacggcgcaa tgatcgattc tcagactgga gacatgattc
     2581 agggcgcgat tgccaacttc agacgggcga ttgcccttgg cacgggagaa atctcgtcgt
     2641 tcgtgtttgt cgatgtgctc aaggatgccg tgaaacgtgg gcagtacttt ctgctgttct
     2701 tcatggatct gcgctcgatc cagggtcact ttttcaacga actgttttta aagcccgaaa
     2761 cctggcctga agacatcgaa ttctataccg acagctggta ctgtccgacg ttgtttcctc
     2821 ggcagggacg accggaaaag ctgcgtcgat ttcttgaacg tctgcagccg ccgcgacgcc
     2881 tgctcagtga aacgacctgg ttgaatggtc gaaaagtgct gctaagcgct ctggccggca
     2941 agaatatccg gcactttgcc gtctttgccg tcaccccctg gaaggtgatc gaagtgcagc
     3001 agaatcgtct gcgacacgag atgtcgatca tcgccatgat gatggccctg ctcatggccg
     3061 cgatttcctg gcgtctgtat cagagcatca tcgccccggc agagcgcttg atggaaggga
     3121 ttcgcgcgat tgaggacaag cggttcgacc atcgtatacc gctcgtgacc ggtgacgaat
     3181 gggacgagtt ggccgaatcg ttcaatcaga cgatcggcag tctcgaggaa ctcgaagttg
     3241 caaaggtggt gcagacacag attctgccgc aggaaacgat tcgtgtgtcc gaggcggagt
     3301 ttgtcggccg cagtctgatg accggccagg ttggtggtga ctacttcgat gccataccca
     3361 acgaggatgg cagtctcagt ttcgtcattg gcgacgtcac cgggcacggc gtttccgcgg
     3421 cgctcgtggt ggccatggtg aaatcggcgt ttcacttcat tcatcgacgc ggcgttcgtg
     3481 cgccggctga aatgctcaag cagatgaatg ccatgattat ccgttcgctc agcaagaaaa
     3541 aagcgctgtc gatgcagatc ggctggtatc agcctggcgg gaaactggtg ctcgcgaatg
     3601 ccggacatcc gtatccgtat ctgttgctgc cacatgcaga ggtgaagccg ctcacggcgc
     3661 ggggaatggt gctgggggtc acggctcgac cccggttcga agaagttacg gtgccgctcg
     3721 attccgcggg cggctcactg gtgctgttca ccgacgggat tgtcgaacag atgaatccgc
     3781 ggggggcacg tgtcagcctg g
//
LOCUS       DAORHD010000338         3797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26453_length_3797_cov_3.924105, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000338 DAORHD010000000
VERSION     DAORHD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26453_length_3797_cov_3.924105"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2829
                     /locus_tag="PKO06_08340"
     CDS             <1..2829
                     /locus_tag="PKO06_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69690.1"
                     /translation="NCLPFFNVYPTLAARDLAWARGAGAPWRSISRREVHLLLCALVA
                     ESIGGTHSMWATLVHWLNLESRHVLGAQVWNSYVERLNYEGQGIFIHQAFRGVSALVA
                     WWPVVTTDQDDDLQRPFLDEPNMLWKRWLTLQTLRGAFLFLADPGEVGEQVGEYCLVS
                     NLRKSGAAALILRQGVPDYSLPGARWFLRSSGLVSALSHARDQIRDFPHLLVSTTTIS
                     RERGTRILIARPLRSSHDVAYSSPLILFGGLLWASALLILWAKAALRTRTIQLSLAHQ
                     LPLFFVLVFLPSLWLSEQVINRSRSESEFNLEETGRRTLNSLFLAMDDSYHLIIQWAV
                     SLFATITHNGDFLTRLAELESNTATGVPAEVTLAPFQRISTPRTQGVSAADDSNPVLR
                     RIAEQWFRFGFSVQYLLAHLPHRAPLGFAMQNSEFSGLKTVGALFSRFAQEHMQRANP
                     NLETAHLDPKKEMLVRAELDEIINVIRNLTPAERSSELFMSPRGFSEILFFKGQTNVL
                     NMGIVRNGVTRYYLQTTAIYTQFLRHLMGNFHRFHHPDLRVFIPLAGSTFDRQNRVYR
                     QPFLQMIRNGGEIDFERFENRQSPDLGQAALMTAFLQDMVWGRSSTNRGEWLYAAQAS
                     QNIAQFLFTGLFPLDQALLELAGTIRRQRALLFAVLAFAITLAFLIARRLLMPLHQLR
                     HACDEVTHERYGIRLPDTRGDEFGELGRVFNFMVTDIASGRLLGTFVSESLRDAVKDM
                     DESLDPQAEVREVTVLFITLSDWKRELSRIDANALIQTLNVYLEQTTRLIRQYGGAID
                     KILGEKVLAVFSATQDVTREIAALNAVRCARHLINYWRHHAPGPQYPPAIGLVSGTAL
                     AGILGTKEVRLDFTVIGDTVNLASRLCDLACTLPQAGLVLDPESISMMQASPGFERQL
                     EFERLPIRSVKGKRREVEAYRLVENPYS"
     gene            2826..>3797
                     /locus_tag="PKO06_08345"
     CDS             2826..>3797
                     /locus_tag="PKO06_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69691.1"
                     /translation="MTKPSRDPFFHLLAWTLIMGITSLLLQHGLQTQVARLYALSQER
                     LADELGQEMRLLQASLSPREQVARYLNDLFETLPISTERTAVEEEHLRQSVTAALQPL
                     LKRLPMFQWYLVGFSPDREQVQMGITHGWERIWKFPLVIDLPESWWNGGLRAFLHLYG
                     SLVFHTSPTTVRFNQFGDILSLFVPSFGFWISDQDEYSRLLAMGKHPERYLSREEAEV
                     FFSELRCRFIGARYDHSLYARHTNRDTQLALFPGNLWWGVNHSMPATGLGQFDTIATS
                     RGSQVFYWLPLFPQSFLSSTRWIDTSSHSAIELQSLQFRNLRGGFFAF"
BASE COUNT          728 a         1137 c         1055 g          877 t
ORIGIN      
        1 aactgcctgc cattcttcaa cgtgtatccc actctggccg cacgagacct cgcctgggcc
       61 agaggagccg gcgctccctg gcgcagtatc tcgcgccgcg aggttcacct gctcttgtgt
      121 gcgctggtcg cggaaagcat cggcggcacc catagcatgt gggcgacatt ggttcactgg
      181 ttgaacctgg aaagccgtca tgtcctggga gctcaggtct ggaactcgta tgtcgagcga
      241 ctcaactacg aggggcaagg gatattcatc catcaggctt ttcggggcgt gagcgctctg
      301 gtcgcctggt ggccggtagt caccaccgat caggatgatg atctgcagcg gccgtttctg
      361 gacgagccaa acatgctgtg gaaaaggtgg cttactctgc agacgttgcg cggagcgttt
      421 ctctttctgg ccgatcccgg cgaggttggc gaacaggttg gcgaatactg tctggtcagc
      481 aatctccgga agagtggcgc ggcagccttg attctgcgac agggagttcc cgattattct
      541 ctgccgggtg cccggtggtt ccttcggtcc tcgggtctgg tttcggcatt gagccatgcc
      601 cgagatcaga tccgggattt tccgcatctg ctcgtcagca ccaccaccat ctcacgcgag
      661 cgcggcaccc ggattctgat tgcccggccg cttcgctcct cccatgatgt tgcgtattca
      721 tcgccgctga ttctcttcgg cggtctcttg tgggcatctg ctctgttgat tctgtgggca
      781 aaagccgctc ttcgcactcg gacgattcag ctctctctcg cgcatcagct gcccctcttt
      841 ttcgtcctcg tgttcctgcc ctctctctgg ttgtccgagc aggtgatcaa tcgatctcgc
      901 tccgagagcg aattcaacct ggaggaaacg ggacgtcgaa cgctgaattc cctttttctg
      961 gccatggatg acagctatca tctgatcatc caatgggccg tctcgctctt tgccacgatc
     1021 actcacaacg gggacttcct gacacgtctc gcggagcttg aatccaacac cgcgaccggt
     1081 gttccggcag aggtcacgct ggccccgttt cagaggatct caacaccacg aacacaaggg
     1141 gtatcggcag cggatgacag caatccggtg cttcgtcgca tcgccgaaca gtggtttcgc
     1201 ttcgggtttt cggttcaata tctgctggcg cacctgcctc atcgcgcccc tctcggattt
     1261 gcgatgcaga attccgagtt ttccggtttg aagacggtcg gagccctctt ttccagattt
     1321 gcccaggaac acatgcagcg ggccaacccc aacctcgaaa ccgcccatct cgaccccaaa
     1381 aaagagatgc tggttcgcgc ggagcttgat gagatcatca acgtgatccg aaacctcacg
     1441 ccggcggagc gttcgtcgga actgttcatg tcaccacgcg ggttcagcga gattctcttc
     1501 ttcaaaggtc agaccaacgt gctcaacatg ggcatcgtgc gaaacggtgt gacccgttac
     1561 tacctgcaga cgaccgccat ctacactcag tttctgcgac atctgatggg caactttcat
     1621 cgatttcacc acccggacct gcgggtcttc attccgctgg ccggcagcac gtttgatcgt
     1681 cagaatcgcg tgtatcgcca acccttcctg caaatgatac ggaatggagg agagatcgat
     1741 ttcgaacggt tcgagaaccg acaatctcct gacctggggc aagccgcgct gatgacggcc
     1801 tttctgcagg atatggtgtg ggggcgttct tcgacaaacc gcggcgagtg gctgtatgcg
     1861 gcacaagcca gccagaacat cgcgcagttt ctctttaccg gactctttcc tctcgatcag
     1921 gcgctgttag agcttgccgg caccattcgc cggcagcgag cacttctgtt cgccgtgctg
     1981 gcattcgcca tcacgctcgc gtttctcatc gcacgaaggt tactcatgcc tttgcaccag
     2041 ctgcgacatg cctgtgacga ggtgacccac gaacgttatg gtatcagatt gcctgatact
     2101 cgaggtgacg agtttggaga actcggacgc gtgttcaatt ttatggtgac cgacatcgct
     2161 tcgggaagac tgctgggaac gttcgtctca gaatcgctgc gcgatgcggt caaggacatg
     2221 gacgagagcc tggatccgca ggccgaggtg cgagaagtca cggttctgtt catcacgctt
     2281 tcggactgga agcgtgagct ctcgaggatt gatgccaacg cactcatcca gactctcaat
     2341 gtctacctcg aacagaccac ccggctgatc cgtcagtatg ggggagcgat cgacaagatt
     2401 ctcggtgaga aggtactggc ggtcttctcg gcaacgcagg acgtgacgcg ggaaatcgcc
     2461 gccctcaatg ccgttcgctg cgcgcggcat ttgatcaact actggcgaca ccatgctcca
     2521 gggccacagt atccgcccgc catcggactc gtcagcggca ccgcgctggc aggaattctc
     2581 ggcaccaagg aagtccgcct ggatttcacg gtcatcggcg acacggtcaa tctggccagt
     2641 cgactctgcg acctggcctg taccctgccc caggccgggc tggttctcga ccctgaatcc
     2701 atcagcatga tgcaggcttc tcccgggttc gaacgtcagc tggaattcga acgcctgccc
     2761 atacgatccg tgaaaggcaa acggcgcgaa gtcgaggcct accggttggt tgagaatccg
     2821 tactcatgac aaagccctcg agagacccgt tttttcatct gctggcctgg actctcatca
     2881 tgggcatcac aagtcttctg ttgcagcatg ggctccagac acaagtggcc cgtctgtatg
     2941 ccctgagcca agaacgactg gccgatgaac taggacagga gatgcgcctg ctgcaagcca
     3001 gcctgtcacc gcgcgaacag gtggcacgat atctgaacga tcttttcgag accctgccga
     3061 tctcgaccga gcggacggcc gtcgaggaag aacacctgcg acagtcagtc accgccgctc
     3121 ttcaacctct gctcaaacga ctgcccatgt ttcagtggta tctcgtcgga ttctctccgg
     3181 atcgcgagca ggtgcagatg ggtatcacgc acggttggga gcgaatctgg aagtttcccc
     3241 tggtgatcga cttgcccgaa agttggtgga acggaggtct tcgggctttt ctccacttgt
     3301 acggctctct ggtttttcac acttcaccaa ccacggtgag gttcaaccaa ttcggcgaca
     3361 ttctcagcct cttcgtgcca tcgttcggct tctggatcag cgatcaggac gagtattcgc
     3421 gtttactggc catgggaaag catccggagc ggtatctctc gcgtgaggag gcagaggtgt
     3481 ttttcagcga gctccgttgt cgattcatcg gtgcccgcta cgatcattcg ctgtatgccc
     3541 gccacaccaa tcgtgacacg cagctggccc tcttccccgg gaatctctgg tggggtgtca
     3601 accactcgat gccggcgacc ggactcggac aattcgatac catcgcgaca agtcgtgggt
     3661 cacaggtgtt ctactggctc ccgttgtttc cccagagttt tctgtcatca acgcgctgga
     3721 tagatacttc ttcccatagc gcgatcgagc tgcaatcact tcagttccgg aacctgcgcg
     3781 gcggcttttt tgcgttc
//
LOCUS       DAORHD010000339         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26482_length_3794_cov_3.502808, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000339 DAORHD010000000
VERSION     DAORHD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26482_length_3794_cov_3.502808"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..2878
                     /gene="ptsP"
                     /locus_tag="PKO06_08350"
     CDS             359..2878
                     /gene="ptsP"
                     /locus_tag="PKO06_08350"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV69692.1"
                     /translation="MAPLSGVMMPLEQVPDPVFAQKLVGDGVSIDPTSQDLLAPCDGR
                     VIQIHACGHALTLQTEQGLEIMMHIGLDTVHLKGKGFAPKVKAGDQVKTGQLLITFDA
                     DAVARQARSLLTQILVTTPERVGSWKRNTGLFEAGRQPLLELVLQGAGATADSAGEGR
                     LLTSEAVVVPNPTGLHARPAAVLVQLARRFKADIRLRKGEGAANARSVVSIMALDVRH
                     GDKILLTAQGVDAEQALALIVPELKNGLGETGSAPVQEATGGKSAQVAAPPVRPRSED
                     PHVLVGVSASPGLAVGSIYRLRHQEIAVVEDGDSPEAERVRLREALEQAKLQLTSLHD
                     RLAMTADAGKAAIFAAHQELLEDPDVLELAESAMAKGKSAAFAWSKAFAVQADRLAAM
                     KNELLAARANDLRDVGRRVLRLITGTTEEKRSFPAETILVAEDLTPSDTANLDRAKVL
                     GFCTVAGGSTSHVAILARSLGIPAIAGIEPRVLETADQTPAVLDGTRGVLRLHPSNEE
                     MTRVKKQQAELAAQREVEIRSAAEPAITTDGHRVAVVANIGGQADSEQSVKLGGEGVG
                     LLRSEFLFIDRNSPPNEDEQTQTYQNMARALGTERTLVIRTLDVGGDKPLPYLPIPRE
                     DNPFLGERGIRVSLDRPEIFRTQLRAILRASVGYRIHVMFPMVASLAEWRAARGQLEV
                     EARALGIPIIPAGIMVEVPAAALLAEHFADEADFFSLGTNDLTQYTLAMDRGHPKLAP
                     HVDGLHPSVLKLIKTTIDGARPRKRSVSVCGGLAGDSRAVPVLIGLGIDKLSVSVPSI
                     PAVKAQIRRLSLRDCQSLAQKALAAGDAAQVRSLVDDALKA"
     gene            2898..>3794
                     /locus_tag="PKO06_08355"
     CDS             2898..>3794
                     /locus_tag="PKO06_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HNV69693.1"
                     /translation="MNLWTQTFALLQKIGKSLMLPVSVLPVAGILLGVGSAEFSWLPT
                     TLSQVMKSAGGAIFGSLPLIFAIGVCLGLTNNDGVAALAGTVGYAVMLATMGVVAKLL
                     GVEVKAVMGFQSIDTGVFGGVLIGGVAAWLFNRYYRVQLPPYLGFFAGKRLVPILTAF
                     AAIAVGVLLSFVWPPIGRGIATFSVWASTGQPAVAFGLYGLIERALIPFGLHHIWNVP
                     FQNVVGEYLIPGTTDIVRGEIPRFVAGDPTAGNLAGGYLFKMWGLPAAAIAMWLCAKK
                     ENQARVGGIMVSAALTSFLTGIT"
BASE COUNT          703 a         1023 c         1249 g          819 t
ORIGIN      
        1 caggcacagg caatgaagag catggcggcc gagatgagca ccttgatcat gggaaaggcc
       61 catccgtaaa cgttttcaca ctcgtcagac ttgttccctc ggcctatctt ccctgccgtc
      121 agaggaattt cctctgagcg gaaggatgct ccatcgatgt gtgccattgt ttgctgacgt
      181 ctgtcgaagc gattggggca cttcctcatt gacgtgacaa agagcagaca cctgtcgaca
      241 aagtcgtgtt tgcccggttc cggtgggagt tgcaaggtat gatagaatgc gaccattggc
      301 tcgtggccgg gaattttcga ggagaggtga tgccgtgacc gaaaaccgcc tggtgttgat
      361 ggctccgctg agtggtgtga tgatgccgct ggaacaggtt ccggaccctg tgttcgcaca
      421 gaagctggtg ggagacggcg tgtcgatcga tcctacctca caggacctac tggcgccctg
      481 cgatggacga gtgattcaga ttcacgcctg cggacatgca ctgacgctgc agaccgaaca
      541 aggcctcgag atcatgatgc acatcggtct cgacacggtt catctcaagg gaaaaggctt
      601 tgcgccgaag gtgaaggccg gcgatcaggt gaagactggc cagctgctga ttacgttcga
      661 tgccgacgct gtcgcacgac aggcgcgcag tttgttgacg cagattctgg tgaccacccc
      721 ggaacgcgtt ggttcttgga aacggaatac cggattgttt gaagccggtc gccaaccttt
      781 gttggaattg gtccttcagg gggcgggtgc gaccgcggac agtgccggcg agggacgcct
      841 gctgacctct gaagctgttg ttgttccgaa tccaacggga ttgcatgccc gcccggctgc
      901 ggtgctggtg cagttggctc ggcgtttcaa ggcggatatt cggttgcgca agggcgaagg
      961 ggcggcgaat gctcgaagtg tcgtctcgat catggccctc gatgttcggc acggtgacaa
     1021 gattctgttg acggctcagg gggtcgatgc cgaacaggct ctggcactga ttgttcccga
     1081 actgaaaaac ggacttggcg aaacgggatc tgcgccggtg caggaagcga ccggtggaaa
     1141 aagtgctcag gtcgcagctc cgcccgttcg tccccggtct gaagatccac atgtgctggt
     1201 gggggtttcg gcctcgcccg gattggcggt cgggagcatc tatcggttgc gacaccagga
     1261 gattgccgtc gtcgaggacg gtgattcgcc cgaagccgag cgcgtgcggc tgcgtgaagc
     1321 gcttgaacag gccaagttgc agttgacgtc gttgcatgac cgtcttgcca tgaccgccga
     1381 tgcgggaaaa gcggccattt ttgccgccca tcaggaactg ctcgaagacc cggatgtgct
     1441 cgaactggct gaaagtgcca tggccaaggg caagagcgcc gcgtttgcct ggagcaaggc
     1501 cttcgccgtg caggcagacc gcctggcagc catgaagaat gagttactgg cggcgcgagc
     1561 caacgatctg cgagatgtcg gcaggcgggt gctgcgcctc ataaccggca ccaccgaaga
     1621 gaaacgctct ttcccggctg agacgattct ggtcgctgag gacctgacac cctctgatac
     1681 ggcaaatctc gaccgagcga aggtgttggg gttctgtacc gttgccggtg ggtcgacctc
     1741 gcacgtggcg attctggctc gttcgcttgg cattccggcg attgcgggaa tcgaaccgcg
     1801 cgttctggaa acggcggacc agactccggc agtgctggat ggaaccaggg gggttctgcg
     1861 tctgcaccct tcgaacgaag agatgacgcg ggtgaagaaa caacaggccg aactggccgc
     1921 acagcgcgaa gtcgagattc ggagcgcggc ggaacccgcg atcacgaccg acggtcatcg
     1981 agtcgccgtt gtggccaaca tcggaggaca ggctgacagc gagcagtcgg tgaagctcgg
     2041 cggagaaggg gttggtttgc tgcgttccga gtttctgttc atcgaccgca actccccgcc
     2101 gaacgaagac gaacaaacac agacctacca gaacatggcc agagctctgg gaacagagcg
     2161 cacgctcgtc attcgaacgc tggatgtggg cggcgacaag ccgcttccct acctgccgat
     2221 tccgcgcgag gacaacccgt ttctcggcga gcgcggtatt cgcgtcagcc tcgaccggcc
     2281 tgagatcttt cgcacccagc tgcgcgccat tcttcgggct tcggtcgggt atcgcatcca
     2341 tgtcatgttt cccatggtgg cgagtctggc cgagtggcgt gcggcgcgtg gtcagctcga
     2401 ggtcgaggcg cgagcgctcg gcattccgat cattcccgcg ggaatcatgg tcgaagtgcc
     2461 ggcggcggcg ttgctggccg agcattttgc tgatgaggcc gacttctttt ctttgggcac
     2521 gaacgatctg acccaataca ccctggcgat ggatcgagga catccgaagt tggcgcctca
     2581 tgtcgatgga ttgcatccgt ccgtgttgaa gttgatcaag acaacgatcg atggcgcgcg
     2641 accgcgtaaa cgatccgtga gtgtctgcgg cggtttggcg ggcgactcgc gggctgtacc
     2701 ggtgctgatc ggtttgggaa tagacaaact cagtgtcagc gtgccgtcga ttccggcggt
     2761 gaaggcgcag attcgtcgtc tgtctctgcg tgactgtcag agtctggcgc agaaagcgct
     2821 ggctgcaggc gatgccgcac aggtccgctc cctggtcgat gatgccttga aggcgtgaag
     2881 gaaagaggaa ccctatgatg aatctctgga cgcaaacctt tgccctgctg cagaaaatcg
     2941 gaaaatcgct catgctgccg gtctcggtgc ttccggtcgc tggtattctc ctgggtgtcg
     3001 gcagcgcgga attttcctgg ttgccgacca cgctgagtca ggtcatgaag tcggcaggtg
     3061 gagccatctt tggttcactg ccgttgattt ttgcgattgg cgtctgcctt ggcctcacca
     3121 acaacgacgg cgtagcggca ctggctggta cggtcgggta tgccgtcatg ctcgcgacaa
     3181 tgggagtcgt ggccaagttg ctcggagttg aagtgaaggc tgtcatgggc tttcagagta
     3241 tcgatacggg ggtgtttggc ggggtgttga tcggcggggt cgcggcctgg ctgttcaacc
     3301 gctactaccg ggtgcagctg ccgccatatc tggggttctt tgccggaaaa cggctggtgc
     3361 cgatcctgac cgcttttgcg gccatcgccg tcggtgtact gctgagtttt gtgtggcccc
     3421 ctatcggtcg cggcatcgcg acgttttcgg tgtgggcttc gacggggcaa ccggcggttg
     3481 ctttcggtct gtacggcttg attgaacgtg ccctgattcc gtttggcctg caccacatct
     3541 ggaatgtgcc gtttcagaac gtggtggggg aatatctcat tccgggcacc acggacatcg
     3601 tgcgtggtga gattccgcgt tttgtggcgg gagatcccac ggcaggcaat ctggccggtg
     3661 gctatctctt caaaatgtgg ggtttgcccg ccgcggcgat tgccatgtgg ctctgtgcga
     3721 aaaaggaaaa tcaggctcgg gttggtggca tcatggtatc tgcagcgctc acctcgttcc
     3781 tgaccggtat cacc
//
LOCUS       DAORHD010000340         3793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26498_length_3793_cov_3.933119, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000340 DAORHD010000000
VERSION     DAORHD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26498_length_3793_cov_3.933119"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PKO06_08360"
     CDS             <1..245
                     /locus_tag="PKO06_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69694.1"
                     /translation="CLTWLEDPDYAGFSQAFQVARQAGLVAGVCRAAASGCAGEGCVR
                     SPIDAARELKLEERHELNGHAGVAEWIERGFELIVF"
     gene            283..1224
                     /locus_tag="PKO06_08365"
     CDS             283..1224
                     /locus_tag="PKO06_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV69695.1"
                     /translation="MIGFIVWFLYNYLWSGIYTVEQGQVAVKTVFGRAQRIEGMTTLQ
                     SAVSINLVEDERQRYAYPQLVVIPPGGPYFKFPWEEVHKVSIATQTLNMAFDPEDPTA
                     NHNGEFIEAVTKDQLNVALTGQIRYRVSEQNLYGYLFGMKNPLVHLMGYFISVLRERI
                     ANYEARRTEDGGAIPVSINDLRKNLHEINEHMMRECQQSAARYGITLEASLITSIDPP
                     DEVEKALAAINTAHNEVSSEISLAQASADQKIVQSRRATEIETLRAKSEVEPLRAMAQ
                     QLAVLKKNGSGVLAAYIRNVRLSLFNKAHKVFLSGGK"
     gene            1224..2150
                     /locus_tag="PKO06_08370"
     CDS             1224..2150
                     /locus_tag="PKO06_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV69696.1"
                     /translation="MENFILGFFFSFIGCYIAIPIFLGIAQSFGFYTIVREGEALIFT
                     LWGQVVGVLKEPGLYFLPWQLGLKAFYIFWAGSCTRLDIRIDQEYLRSQQVNSEEGAP
                     MGIGIWYEMLITDPVAFLFKNANPRSSLAANVRSAVVRCLSNMKLADMLEDRHQMSQA
                     VRDEVSPQSQEWGYRLGSVYVRKVHFRDKRMIGQIEEKVVNRLRQVTSAIKQDGTNQV
                     NIITSTAENTAAVEFAKAAAMRPLIVGEALREISQDPEILQALFEILEAQRLVEGEGE
                     IVLLPTDSGLGRQIALLDAANSVNALPDSKPT"
     gene            complement(2215..>3793)
                     /locus_tag="PKO06_08375"
     CDS             complement(2215..>3793)
                     /locus_tag="PKO06_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV69697.1"
                     /translation="SVQPARAAAPAMTGPTAAVAAAPVSRTALTTAPPITRGIPGNPR
                     LAALVKQSPSTASDVAPDSDKPVSAKDNPTQTVGARNRSELIAKLENLRQRAKSAYHT
                     VAQPEAAAAPADEHVDFAADLSGNPEVLADLPTAESTSETTDSSTADSGSPLSIVQVI
                     KSLPMKPWEFDAGLNRYVVRYDNQTTVILTLRPALQRLVEQTFRSTSCRLGAAIIQDP
                     TSGAILAMTSFDGQTALSPVNTDFVNNNWALKATFPVASIFKIITAAAAFDKRMASPE
                     TRVKIGRKAIIELWRAFAKSHNGTFAAIGRKVGQQVMQMTANAFGFNQAFYFDLPVTQ
                     SVAQMPDNLTKLGEASAGLNKTFDVSPLHVSSIVSTVLNDGKMMKPYLVEAIMHQGKV
                     VFRRKSFQLGRPVTPGSAQQIVKMMKTTTTDGTGKKGFNGYFDCPALASQCGGKTGTL
                     TGVNPHLLFTWWGGFTQVGSRLLAMTVLVGQPPSTSRTKAPSVAGRLAYSLLNNKPAP
                     TAAPIVTASRKHYRRKR"
BASE COUNT          777 a          990 c         1156 g          870 t
ORIGIN      
        1 gctgtctgac ctggttggaa gaccccgact atgcgggctt ttcgcaggca tttcaggttg
       61 cccggcaggc cggtcttgtg gcgggagtct gtcgagcggc cgcctccggg tgtgccgggg
      121 aaggctgtgt tcgctcaccg atcgacgcgg ctcgtgaact gaaacttgag gaacggcatg
      181 agttgaatgg ccatgcgggt gttgctgaat ggatcgaacg agggttcgaa ctgatcgttt
      241 tctgaaaggg agctgaatat gggtggtttc ttcgcaggct gcatgatcgg tttcattgtc
      301 tggttcttgt acaattatct ctggtcgggt atctataccg ttgagcaggg gcaggtagcc
      361 gtcaagaccg tgttcggacg ggcgcagcgc atcgaaggca tgacaacgct gcagtcggcg
      421 gtttcgatca atctggtcga agatgagcgt cagcggtatg cctatccgca actggtcgtc
      481 attcccccgg gcggaccgta tttcaagttc ccgtgggaag aagtgcataa ggtttcaatt
      541 gccacgcaga cactgaacat ggcctttgac cctgaagatc caacagctaa tcataacggc
      601 gagtttatcg aggccgtaac caaggatcaa ttgaacgtcg ccttgaccgg tcagattcgc
      661 tatcgagtca gcgagcagaa tctttatggc tatctgttcg gcatgaagaa tccgctggtg
      721 catctgatgg gatacttcat ttccgttcta cgcgaacgaa ttgccaacta cgaagccagg
      781 cgcacggagg acggcggagc gattcccgtt tctatcaatg atttgcgcaa gaacctgcat
      841 gaaatcaacg agcatatgat gcgcgagtgc cagcagtcag ccgcgcgata tggcattacg
      901 cttgaagctt cgttgatcac cagtatcgat ccgcctgatg aagtcgagaa agccctggcg
      961 gctatcaata ctgcgcacaa cgaggtttcc tccgagatca gtctggcgca ggcctcggcg
     1021 gatcagaaga tcgttcaatc acggcgggcg acggaaatcg agaccctgcg cgcgaaatcc
     1081 gaggtcgagc cgttgcgtgc catggctcag cagctggcgg ttctcaagaa gaatggttcg
     1141 ggagtcctgg cagcatacat tcgaaacgtc cgtctgagcc tctttaacaa ggcacacaaa
     1201 gtgttcttgt cgggaggcaa gtgatggaaa acttcattct gggctttttc ttttccttca
     1261 tcggctgtta catcgcgatt ccgatctttc tcggcatcgc ccagagtttc gggttctaca
     1321 cgattgtccg cgaaggcgaa gcgttgatct ttacgctgtg gggacaggtc gtcggtgttc
     1381 tcaaggaacc tggcttgtac tttctgccgt ggcaactggg actgaaggcg ttttacatct
     1441 tctgggccgg tagttgtacc aggcttgata ttcgcatcga tcaggaatat ctgcgcagtc
     1501 agcaggtcaa ttccgaagaa ggagcgccca tgggtatcgg tatctggtat gagatgctga
     1561 ttaccgaccc ggtggccttt ctgttcaaga atgccaaccc ccgctcatcg ctcgcggcga
     1621 acgttcgcag tgcggtcgta cgttgtctca gcaacatgaa actggctgac atgctcgaag
     1681 atcggcacca gatgagccag gcagtgcgag atgaggtgtc gccgcagtcg caggaatggg
     1741 gatatcggct cggctcggtc tacgtgcgaa aagtgcattt tcgcgacaaa cggatgatcg
     1801 gccagatcga ggagaaggtg gtgaaccgct tgcgtcaggt tacctccgcg atcaagcagg
     1861 atggcaccaa ccaggtgaac atcattacca gtactgccga gaataccgct gcggtggagt
     1921 tcgcgaaagc ggccgcgatg cgaccattga tcgtcggtga ggccctgcgt gagatcagtc
     1981 aggatccgga gattctgcag gccctgttcg agattctcga agcccagcga cttgtcgagg
     2041 gcgagggcga gattgttctg ctgccgacag attcgggtct tggcaggcag attgcccttc
     2101 ttgacgccgc gaactcggtg aatgctctcc cagactcaaa accaacataa gagctgaaag
     2161 cagaagcggg ggtgcaaacc cccgcttctg catgtctggc acgttttgct ggtgtcagcg
     2221 ttttcgtcga taatgcttgc gactggccgt cacgatcggt gcggctgtgg gtgccggttt
     2281 gttgttcagc aggctgtagg ccagacggcc ggcaacgctt ggagccttgg tgcgactggt
     2341 actgggtggt tgaccgacca gaaccgtcat ggcgagcaga cggctgccga cctgagtgaa
     2401 tccgccccac cacgtaaaca gcagatgagg attaacgccg gtgagggttc ccgtcttgcc
     2461 gccgcattgt gaggcgagag ccggacagtc gaaatacccg ttgaatccct tcttcccggt
     2521 gccatccgtc gtggtggtct tcatcatctt gacgatctgc tgcgccgaac ccggagtgac
     2581 cggacgtccc aactggaatg acttgcgtcg aaaaactacc ttgccctgat gcatgatggc
     2641 ttcaaccaga tagggtttca tcatttttcc gtcgttcaga accgtcgata cgattgagga
     2701 cacatgcaac ggactgacgt cgaaggtttt gttcaacccc gccgaagcct ctcccagttt
     2761 cgtcaggtta tcaggcatct gcgcaaccga ctgggtcacc gggagatcga agtagaatgc
     2821 ctgattgaag ccgaatgcgt tggcagtcat ttgcatcacc tgctggccga ctttgcgacc
     2881 aatagcggca aaagtaccat tgtgtgattt cgcgaaagcg cgccacaact cgatgatcgc
     2941 cttgcgacca atcttcactc gtgtctcggg cgaagccatt cgcttgtcga acgctgctgc
     3001 cgccgtaatg attttgaaaa tcgaagcaac aggaaaggtc gctttcaggg cccagttgtt
     3061 attgacaaag tcagtgttta ccggcgagag cgcggtctga ccatcgaacg aggtcatggc
     3121 cagaatggct cccgaggtcg gatcctgaat gatggccgct ccgagacgac aggaagtgct
     3181 gcggaacgtc tgctcgacca gacgctgtaa cgccggccgc agtgtcagaa tgaccgtggt
     3241 ctggttgtcg tatcgtacga cgtagcgatt cagccctgca tcgaactccc agggcttcat
     3301 cggcagagac ttgatgacct gcacgatgga aagcggactg ccactgtctg cggtgctcga
     3361 gtctgtggtt tcgctcgtac tctccgcggt cggaagatcg gcgagcactt ccgggtttcc
     3421 tgacaggtcg gcggcgaaat cgacgtgctc gtcagctggt gctgctgcgg cctcgggttg
     3481 ggccaccgtg tgataggcac tcttggctcg ttgacgcagg ttctcgagtt ttgcgatcaa
     3541 ttcgctgcga ttgcgcgctc cgaccgtttg tgtcgggtta tcctttgctg aaacgggttt
     3601 gtcagaatct ggagccacat ctgatgccgt cgacggcgat tgtttgacca gcgcggccag
     3661 acgcgggttt cccggtatgc cgcgcgtgat cggtggtgcg gtggtgagcg cggtgcgaga
     3721 gacgggggcg gcagctactg ccgccgttgg tccggtcatg gcaggtgcag cggcacgggc
     3781 gggctgtacg gaa
//
LOCUS       DAORHD010000341         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26534_length_3789_cov_3.665238, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000341 DAORHD010000000
VERSION     DAORHD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26534_length_3789_cov_3.665238"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKO06_08380"
     CDS             complement(<1..278)
                     /locus_tag="PKO06_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69698.1"
                     /translation="MKHSYWLPLLLGIGMAITFIAVNQYEQRNKRKPSFFNPYRPMPP
                     LTGGAARPVPSRSFPPPRHFPPPRSFPPQPATPPVPIVAPYPTHAIPVP"
     gene            complement(475..1044)
                     /locus_tag="PKO06_08385"
     CDS             complement(475..1044)
                     /locus_tag="PKO06_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69699.1"
                     /translation="MSGLQAVLLKAAASTVVSEELIAHRSNFAEKMGFYLEDSERQFL
                     NAVAERELRGMIADIEVPEPKNEIVSSWVSTVLSYSVLAIVIALAAIAWPARPTKFRA
                     PHEKACYANMREILGAIEMYNMQNQRKFTILDRFSMQKLQETGCLRQIPECPEYVEKI
                     VTRQSGEEYAGKDLDISGHIACKMHGTIE"
     gene            1345..2874
                     /locus_tag="PKO06_08390"
     CDS             1345..2874
                     /locus_tag="PKO06_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV69700.1"
                     /translation="MKKPLTLMCTPTPEAMLAAAVCWKKDTELVSVGSKWINEALAKI
                     PADASHREIIILLDDVAEPETTRKHLRALMKRGITITWFGSTRYERLQKELVSVKGLS
                     FVLGKGVLNAVLSKYQPSSELMRVIKAYQGYNEDLFHTLNYFKTRFMMNFDDPRELQA
                     AIEFLHSWDAEKFSIGTLPPVLREAITNFREADFPYIEGKSERVLELKKRILQVANTD
                     LSALILGETGTGKEAVAFFLHDFSTRRGKPFVAINCAGLDETFLRSELFGHKRGAFTG
                     AISDHAGLVKAAHGGTLFLDEVTEMPPAIQADLLRFLQNRRFRPLGQQREEVADVRLV
                     AAAQPSLYQKLREGSFRSDLFFRLNEVDLKTMPLREIPDDIMRVVSHLVFRLYRKHGI
                     NIPETLVSFEQQADAMRSYSWPGNVRELYSLVRQHGLLKTDILASLQQSQNLWSANQP
                     SPANTFSVPLNPIRPLDRVRAEYVQSLVNAHPDMTRKSLAQALEIAPNTLAALLRNAL
                     K"
     gene            complement(2907..>3789)
                     /locus_tag="PKO06_08395"
     CDS             complement(2907..>3789)
                     /locus_tag="PKO06_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69701.1"
                     /translation="YDIYLLTFPIPYAPASWVTTAPSLIATVAPGIYELHITARVASA
                     SIGTLLFHSTRNAYTPNGMFVGLMHRPFSMHVVPSATEPRSVVVNLRYGNQPYDLPPE
                     AQVYATLKERKGTASYSVPVNVGSQSLRVSSMKPGMYRLDLLIDRNQSNPPGFSGDLF
                     GRINLGVNADDVETTMLLTSIMRLRYPEDSDTPGLGDRLERNELVEMENPVTFAWEPL
                     SDTATYTWSLSFGAHGTTTATAIVVDAQPGQNLHLNLSGRDASGTMGQLRIGYPTDRP
                     FYNFCTRPASDSPPIGR"
BASE COUNT          837 a         1007 c         1074 g          871 t
ORIGIN      
        1 ggaactggta tggcatgggt tggataagga gccacgatcg gcaccggcgg ggttgctggt
       61 tgcggaggga agctacgcgg tggaggaaag tggcggggtg gaggaaagct acgcgatgga
      121 accggtcgcg cggcaccccc ggtaagtggg ggcatgggac ggtaggggtt gaaaaacgat
      181 ggctttctct tgttacgctg ttcatactgg tttacagcga taaaggtaat tgccatcccg
      241 attcccagca gcagcggtaa ccagtaactg tgtttcatgt gtgtctcaag ccaagtgtat
      301 cacttctacg cggaacaagg agcgcaggag agtcgcgttc aaaacaaacg cgagaaatcc
      361 gttatggaga aagcattcgg atggtctcgg gtgcgcaacg cgcaggcaac aggtatcgcc
      421 gattcctgac tgaaagttgc tattgagcaa caaaaaccat gggctgtcgg aactctactc
      481 gatggtcccg tgcatcttgc aggcaatgtg cccggagata tccaggtctt ttccagcata
      541 ctcttcacct gattggcgcg tgacaatctt ttcgacgtat tctggacatt ccggtatctg
      601 cctgaggcag ccagtttctt gcagtttctg catcgagaac ctgtcgagga tcgtaaactt
      661 cctctgattc tgcatattgt acatttcgat ggctccgaga atctctcgca tatttgcata
      721 gcaagctttt tcgtgaggag cacgaaactt tgtcggtcgg gcaggccagg ctattgcagc
      781 taatgctatg actattgcga gaaccgaata tgacagaacc gtgctgaccc acgatgatac
      841 gatttcgttt ttcggttctg gaacttcaat gtcagcgatc atcccgcgta gctcacgctc
      901 cgcaacggca ttcaaaaact gcctctctga gtcctcaaga tagaaaccca ttttctctgc
      961 aaagttgctt cggtgcgcta tcaattcttc gcttacgact gtgcttgctg cagccttgag
     1021 taacacggct tgtagcccgc tcatacctcc tgtcgcacga ggttggccag gttccgcttt
     1081 catgtttcca aggttctgtg tcctgtctcg atcggtagta gtacccatgc gagttgaaat
     1141 ctcctcttcc agatacgagg tccaatttct gcacgctgca aactgtagat tgatgctatc
     1201 acagagactg tcgaaaaaaa cacgaccgag atcttcagaa acaaatcaga cactgtcttc
     1261 aatattcgat atcatagctc aatatttgcg tctcttcagc atgcgtgtta cgtttcaact
     1321 atccgatgaa ggtgagtgaa tcgcatgaaa aagccgttga cgttgatgtg tacccctacc
     1381 cccgaggcca tgctggcggc cgcggtctgc tggaagaagg ataccgaact ggtttcggtc
     1441 ggttcgaagt ggatcaacga ggccctggcg aagattccgg cagatgcttc gcatcgcgag
     1501 atcatcattc tgctcgatga cgtcgccgag ccggaaacca ctcgaaagca tctccgcgcg
     1561 ctcatgaaac gcggaatcac gatcacctgg ttcgggtcga ctcgctacga gagattgcag
     1621 aaagagctgg tgtctgtgaa gggtctgtcc ttcgtgctcg gcaagggagt gctcaatgcg
     1681 gttctcagta agtaccagcc gtcgtccgag ctgatgcgtg tcataaaggc ctaccagggg
     1741 tataacgaag acctgtttca tacgctgaac tacttcaaga cccgattcat gatgaacttc
     1801 gatgacccgc gcgagcttca agccgcgatc gagtttctgc actcgtggga cgccgagaag
     1861 ttttcgattg gcaccctgcc cccggttctg cgcgaggcca tcactaattt tcgtgaagcc
     1921 gactttccct acatcgaggg aaaatcagag cgggtgctcg aactgaagaa gcgtattctg
     1981 caggtggcga acaccgatct gagcgctttg attctcggcg aaaccggtac cggcaaagaa
     2041 gcagtcgcct tttttctgca tgacttcagt acccggcgcg gcaagccgtt tgtcgccatc
     2101 aactgtgccg gcctcgacga aacgtttttg cgctcagagc tgtttgggca caaacgcggc
     2161 gcgtttacag gtgcgatcag cgatcacgcc ggcctggtca aggcggccca cggaggtacc
     2221 ctgtttctcg atgaggtcac cgagatgcca ccagcgattc aggctgattt gctgcgattt
     2281 ctgcagaatc gccgctttcg gcctctcggt cagcaaagag aagaggtcgc cgacgtgcgt
     2341 ctcgtggctg ccgctcagcc ctcgctgtat caaaaattgc gtgagggctc gttccgttct
     2401 gatctcttct tccgcctgaa cgaagtggac ctgaaaacca tgccgctgcg cgagatcccc
     2461 gacgacatca tgcgcgtcgt cagccacctg gtcttccgcc tctaccgcaa gcatggcatc
     2521 aacattcccg aaacactcgt ttcatttgaa cagcaggcgg atgcaatgcg atcgtattcg
     2581 tggcccggca acgtgcgcga actgtactcg cttgttcgac aacacggtct cctgaaaacc
     2641 gacatcctcg ccagtctgca gcagagccag aacctctggt cagcgaacca gccgagcccg
     2701 gcgaacacct tttccgtgcc actgaacccg attcgccccc tcgaccgcgt ccgcgccgag
     2761 tatgtccagt ctctggtcaa tgcgcatcca gacatgacca ggaaatcact cgcgcaagcc
     2821 ctcgagattg ccccgaacac tctggccgct cttctgagga acgccttgaa gtaaaagtcg
     2881 gtcttcggat cgtgtaaagg cgagcgttac cgcccaatcg ggggcgagtc ggaggcgggt
     2941 cgggtgcaga agttgtagaa ggggcggtcg gtcggatagc cgatgcgcag ctgtcccatc
     3001 gtaccggaag cgtcgcggcc tgagagattg agatgcaggt tttggccggg ttgggcatcg
     3061 acgacgatgg ccgtcgcggt ggttgtgcca tgggcaccga agctgaggct ccaggtgtag
     3121 gtggcagtat cgctgagcgg ttcccaggcg aaggtaacgg gattttccat ttcaaccagt
     3181 tcatttctct cgagacggtc gcccaagccg ggggtgtccg agtcttcggg gtagcgcaag
     3241 cgcataatgc tggtcagcag catcgttgtc tcgacatcgt ccgcattcac tccgagattg
     3301 atccttccga acagatcacc gctgaaaccg ggcgggttgc tctgatttcg atcgatgagc
     3361 aagtcaagac gatacatacc aggcttcatg gagctgactc tgagcgactg tgatccgaca
     3421 ttaactggta cggaatatga agcagtgccc ttccgttctt tcagtgtcgc gtacacctgc
     3481 gcttcgggtg gaagatcgta tggttgattg ccataacgaa gattgacgac gactgatctt
     3541 ggctcggtcg cactcggaac aacgtgcatg gaaaacgggc ggtgcatgag accgacaaac
     3601 atcccgttcg gggtgtaggc gttgcgagtc gagtggaaaa gaagggtgcc gatcgatgca
     3661 ctggcgacac gagccgtaat atgcagttcg tagattccgg gcgcgacggt ggcgatcagt
     3721 gagggcgctg tcgtcaccca gcttgccggg gcatatggaa ttgggaacgt gaggaggtag
     3781 atatcgtaa
//
LOCUS       DAORHD010000342         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26556_length_3787_cov_3.200429, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000342 DAORHD010000000
VERSION     DAORHD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26556_length_3787_cov_3.200429"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PKO06_08400"
     CDS             complement(<1..187)
                     /locus_tag="PKO06_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69702.1"
                     /translation="MKHLLNFVPSTRFSGQSSFIYRTILGLFYALPLMFATYWTLAYW
                     GYSDIIAELEDASKRLEE"
     gene            complement(184..1275)
                     /gene="pilM"
                     /locus_tag="PKO06_08405"
     CDS             complement(184..1275)
                     /gene="pilM"
                     /locus_tag="PKO06_08405"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNV69703.1"
                     /translation="MKLLHEYAALDLGTYSAKLLALQSADGYTYEISDLHESVLPTGM
                     ISGGFTNSMISDQDKFEITLSQLLGKLSRRKAGLIVGLPDRWVKLHLVALQVRAEELE
                     SRDYLNWRLQKAIGSADKSAELMTDCQVLSTREEGNLLAVKCLVGQVRKEPVEALSQI
                     LMRLKIPVVAFDTSTLGVFNLLEETHPDLTIDRDLIMCHIGHETTVVKAFEQGILRYE
                     RVIEVGGEAFTSLLAEAENLSLELAGAEKLKRAFFPMTREEMVRQIAQRHLFEKVFGN
                     WLRELTVTFRFYQDKFKVLKIPHLYLSGGSSLFEGLPEFLSEFFETTCRRFNPLKELS
                     SSPQPGVDFRTQGPVFAPALGLLVTEGSV"
     gene            complement(1272..3167)
                     /locus_tag="PKO06_08410"
     CDS             complement(1272..3167)
                     /locus_tag="PKO06_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV69704.1"
                     /translation="MDSLKQFLTEHKLADADRVRALEEQAAQSNRSLDRLLIDEASIA
                     NEDLRRAYIRSFKKKPLYEILIEQGLITFELAQQIADEFGAMPTNLGKILVERKLISE
                     EDNAKAMARELGLEYLDLTDYQVNDALFNSIDLALMRDYSFIPHHRGEKEIELIMADP
                     GNVDAIEALEVKLGLPIVPRVASHRSIQRLIALLVETSLDSKMVFSDTEQLPPLEVII
                     DKDAEESAPPPGVAVQKELGEEAPVIRLVDSIILKGVRKRASDIHFETYESQLKVKYR
                     IDGVLFEVLSDVEPRLRKAVVSRLKVMAQLDIAEKRVPQDGRFKLKIDERFVDFRVSV
                     LPCIFGETVVLRILDKSALGLDIRKLGLEGNDLTVFQRNVRKPYGMLLVAGPTGSGKT
                     TTLYSAIKFVHTPGQKFVTIEDPVEYQLPDIVQVAVNEKKGMTFSSGLRSILRQDPNK
                     IMVGEIRDPETAHIAINAALTGHLVLSTIHANNVIDAISRLTNMKIDPYEFVSSFNLI
                     MSQRLVRIICPNCKIEDTTITQEAKDLTPDYVEHAQANFLRGKGCRYCHDMGYQGRKG
                     LFEVLEMSPQIKQMVLNKESPLKIKSQALSAGMTTLRQAGWKRVENGETTFEELNRVT
                     FEEGILQ"
     gene            complement(3183..>3787)
                     /locus_tag="PKO06_08415"
     CDS             complement(3183..>3787)
                     /locus_tag="PKO06_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV69705.1"
                     /translation="THFLGETIDIHSGGSDLVFPHHESEIAQTEAVTQREPFARCWLH
                     TAMVRYEGEKMSKSLGNLVMVTDLLKTYSADSVRLYLGQHHYRTAWSYREAELVQAEQ
                     LAQKLRAAVLASGGAGPQLDASPSEHAFKLALDQDLDTPKALAVLENLADHYDEEIDS
                     LTSTVTALLEPILFLGMALVLGSVILAILLPVLSAAGQIK"
BASE COUNT          877 a         1072 c          973 g          865 t
ORIGIN      
        1 tctcctccag ccgtttgctg gcatcctcaa gttcggcgat aatgtcgctg tatccccaat
       61 aggccagcgt ccagtaggtg gcaaacatca ggggcaatgc atagaacagg cccagaatcg
      121 ttcgatagat aaaactgctc tgcccgctga atctcgtgga cggcacgaag ttgagtagat
      181 gtttcataca ctcccctcag tcaccagcag accaagtgcc ggcgcaaaca ccggcccctg
      241 ggtgcggaaa tcgactccag gctgaggtga ggacgacagc tcttttaatg ggttgaatcg
      301 tcgacaggtg gtttcgaaaa attcactgag aaactccggc aacccttcga agagactgga
      361 tccgccgctc agatagagat gcggaatctt caagactttg aatttatcct gatagaaacg
      421 gaaggtcacc gtcagctcgc gcaaccagtt cccgaacacc ttctcaaaga gatgtcgctg
      481 cgcaatttgc cgcaccattt cttcacgagt catcggaaag aacgctcgtt tcagcttttc
      541 cgcacccgcc agttcgagcg acaggttttc cgcttcggcc agcaacgagg taaacgcctc
      601 gccgccaacc tcgatcactc tttcgtaacg cagaattccc tgctcgaatg ccttgacaac
      661 ggtcgtctca tgaccgatat ggcacatgat caggtcgcga tcgatcgtga gatccgggtg
      721 agtttcctcg agcagattga agactccgag cgtcgaggta tcgaatgcca ccaccggaat
      781 cttcagacgc atcagaattt gcgaaagcgc ttccaccggt tcttttcgaa cctgtccaac
      841 cagacactta accgcaagca gattgccttc ttcgcgagtc gagagcacct gacagtcggt
      901 catcagttcc gccgatttat ccgcgctccc gatcgctttc tgcaagcgcc aattcagata
      961 gtcacgcgac tcgagttcct cggcccgcac ctgcaaagca acgagatgca gcttcaccca
     1021 gcgatcgggc agcccgacaa tcaatcccgc ctttcgtctc gaaagtttcc ccaacaactg
     1081 cgagagggtt atctcgaatt tgtcctgatc gctgatcatc gagttggtga atccacccga
     1141 gatcatgcca gtgggaagca cggattcgtg caaatcggag atctcatacg tgtaaccgtc
     1201 agctgactgc agcgccaaca atttcgcgct gtaagtgccg agatcgagag cagcgtattc
     1261 gtgcagcaat ttcattgcag aattccctcc tcgaaggtga cccggttcaa ctcttcgaag
     1321 gtggtctctc cgttctcaac ccgcttccag ccagcctgac gcagggtggt catccctgcc
     1381 gacaacgcct gagatttgat tttgagcggg gattccttgt tcagaaccat ctgcttgatc
     1441 tgaggcgaca tttcgagaac ttcaaacagg cccttgcggc cctgataccc catatcgtgg
     1501 caataacgac accccttgcc gcgcaggaaa ttcgcttgcg cgtgttcgac gtaatccggc
     1561 gtgaggtcct tcgcttcctg cgtgatagtc gtgtcctcta tcttgcagtt ggggcagata
     1621 atgcgaacaa gccgctggga catgatcaga ttaaaagaac tcacgaactc atacggatcg
     1681 atcttcatgt tcgtcagtcg cgaaatggcg tcgatgacat tgttcgcatg aattgtgctc
     1741 agaaccagat gcccggtgag tgcggcattg atcgcgatgt gggctgtttc cgggtcacga
     1801 atttcaccca ccatgatttt gtttggatcc tggcggagaa tcgaacgaag accggaactg
     1861 aaagtcatgc cctttttctc gttcacggcc acctggacaa tatcgggaag ctggtattcc
     1921 acagggtctt caatggtcac aaacttctga ccgggagtat gcacgaattt gatggccgag
     1981 tacagagtgg tcgttttgcc ggagccggtc ggaccggcga ccagcaacat gccatagggc
     2041 ttgcgaacat tgcgttgaaa gaccgtgaga tcgttgccct cgaggccaag tttgcgaatg
     2101 tcgagtccaa gagccgattt gtccagaata cgcaggacaa cggtttcccc aaagatgcac
     2161 ggaagaaccg agacgcgaaa gtcgacgaat cgctcatcaa ttttgagttt gaaacgcccg
     2221 tcctgcggta cccgcttttc cgcaatgtcg agctgcgcca tgaccttgag gcgcgaaacc
     2281 accgctttgc gtaaccgggg ttcgacatcg gatagcactt cgaacagcac gccgtcaatg
     2341 cgatacttca ctttgagctg cgattcatag gtttcgaagt gaatgtcgct ggcgcgcttg
     2401 cgcacccctt tgagaatgat cgaatcgacg agccgaatca cgggagcttc ttcgcccaac
     2461 tctttctgaa cggcgactcc ggggggggga gcgctttctt ccgcgtcctt gtcgatgatg
     2521 acctcgagtg gcggtagctg ttcggtgtcc gaaaacacca tcttcgagtc caggctggtt
     2581 tcaaccaaca atgcgatcag acgctgaatc gagcgatgag aagcgacccg tggcacgatc
     2641 ggcaatccca gcttgacctc gagggcctcg attgcatcaa catttcccgg gtcggccatg
     2701 atcaactcga tctctttctc gccgcgatga tgcggaatga acgaataatc acgcatcagc
     2761 gccagatcga tgctgttgaa cagggcatca ttaacctggt aatccgtcag atccaggtat
     2821 tccaggccaa gttcgcgcgc catggccttg gcgttgtctt cctccgagat cagctttcgc
     2881 tcgaccagaa tcttgccgag attggtcggc atggctccga actcgtcggc aatctgttgt
     2941 gccagttcaa aggtgatcaa tccctgctcg atcaaaatct cgtagagtgg cttcttctta
     3001 aaagatcgaa tataggcacg tcgtagatct tcgttggcaa tcgaagcttc atcgatcagg
     3061 agtcgatcga gggatcgatt ggactgcgcc gcctgctctt caagcgcgcg cacccggtcc
     3121 gcatctgcca gtttgtgttc cgtcaggaac tgtttcagag aatccatgat ctgtatctca
     3181 cctcacttga tctgcccagc agctgacagg acgggcaaca ggatggccag aatgacggaa
     3241 ccaagaacca acgccattcc cagaaacaga atcggctcaa gcaacgccgt tactgttgag
     3301 gtcagggagt cgatctcttc gtcatagtga tcggccagat tttcgagcac agcaagcgct
     3361 ttaggtgtat ccagatcctg atccaacgcc aatttgaacg cgtgctcgct aggcgacgca
     3421 tccagttggg ggccagcgcc accactggct agcaccgctg cacgcaactt ttgggccagt
     3481 tgctcggctt ggacgagttc ggcttcgcga tagctccagg ctgttcgata atgatgctga
     3541 cccaaataaa gccgcacgct atcggcggaa taggtcttga gcagatccgt gaccatgacc
     3601 aaattaccca acgacttgct catcttctcg ccttcatagc gcaccatcgc ggtatgcagc
     3661 cagcagcgag caaacggctc acgctgcgtg accgcctcgg tctgggcaat ttcgctttca
     3721 tgatgcggaa aaacgagatc actgcccccg ctatgaatat caatcgtctc acccagaaaa
     3781 tgggttg
//
LOCUS       DAORHD010000343         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26585_length_3784_cov_3.516492, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000343 DAORHD010000000
VERSION     DAORHD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26585_length_3784_cov_3.516492"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PKO06_08420"
     CDS             complement(<1..468)
                     /locus_tag="PKO06_08420"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV69706.1"
                     /translation="MPVLSAFRSVVLRTLVLSLFLAVAAVALAATASAPVTTPTASCL
                     ACHSVNQPNLVSSWKKGRHAADGVGCYECHQAADGDPDAMVDHYGFTVSAIVSPKDCA
                     RCHPSQAASFLKSHHASAAVLLRDQTGTYKAENGDVQFGGVVQGYASFINGCES"
     gene            complement(473..2017)
                     /locus_tag="PKO06_08425"
     CDS             complement(473..2017)
                     /locus_tag="PKO06_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV69707.1"
                     /translation="MRARHLVMAILWVGLTTPAMAVADAKGLYTTRAVATTETQKCLA
                     CHTQMNPNLCMSWERGRHAEKAVGCYECHVASATDIDAIPDHHGFTISTLVTPFDCAR
                     CHKEQADSFLNSHHAKGGRVLHSLSNFWGEVVEGYAASLNGCQSCHGSKVHIDPETRK
                     PLANGYPNHGIGRLNPDGSEGNCVACHQRHDFKMSAARRAEVCGRCHMGPDHPQIEIW
                     LESKHGNAYDELNRGVDLNAPKLTLGKDHIGAPSCYTCHFGATNASGSVSSHDPAHRL
                     SWNLRDAISVRRDNWREKRQAMEGVCNRCHSPTYVKNHFIQLEKAVALYNHKFALPAK
                     KILDTLKAAGKLDESPVNDKLERDWWHLWHHQGRRARHGVAMMGADYVQWQGFYDVAL
                     IFYFEFLPEAERLMPGITKPVLDKIEHQWLRDRISKPEEVKKAIQESVRFWYEQIPEP
                     KSEGVLPPGYREGEIKPVEYPPADSGSGSQALPNPTPQPASDAAPTPTPVSPASSAVP
                     VTPGAR"
     gene            complement(2070..3161)
                     /locus_tag="PKO06_08430"
     CDS             complement(2070..3161)
                     /locus_tag="PKO06_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV69708.1"
                     /translation="MTTLLLSLLTMNGLILGLCLLLWASRAWLSSYGEAKIVINGEKT
                     ITVQGGNSLLMSLVMNKVFIPSACGGKGTCGYCRVKVHEGGGSVLATEQMVLSPQEVR
                     EQKRLACQVRVRRDLVIEVPEEYLAIKEYQVELAQVEVMTDLIKKLTFRLLEPKQITF
                     KPGQYVQACREMPDDEPLYRGYSMANSPLRPDVIELNVRLVEGGMMSPYLHSLKVGDQ
                     LRMSGPYGDFFLQEKTDRSVVCVAGGVGLAPMKSIIRYITEKRIQRKVYLFYGARTLP
                     LLYDHQDFVDLAKANPNFIYVPALSDDNVGADWQGHRGFITTVFSKVFPEGEPAEAYL
                     CGPPVMIDALLPLLKQKGIQSQDIYYDKF"
     gene            complement(3163..3753)
                     /locus_tag="PKO06_08435"
     CDS             complement(3163..3753)
                     /locus_tag="PKO06_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNV69709.1"
                     /translation="MGDPIRLLVIFFASCLTNNIALAYFIGMCAFLALSRNVSVAFGM
                     GVAVTFVTALTAMVNWLINYFILAPLGLEFFQFLVFIIVIAAIVQFLELLMDRFFPGL
                     YEAFGIYLPLITVNCTVLAASLFMVFRQYGFWETTVFSFGTGVGWLLAIVLLAAIRQH
                     LIFARPVRQLGDVGMALLIAGLMAMAFSGFSGMVKL"
BASE COUNT          794 a         1153 c         1104 g          733 t
ORIGIN      
        1 ggattcgcag ccgttgatga aagaggcata cccctgcacc acccctccga actgcacatc
       61 tccattttcc gccttgtacg ttcccgtctg atcacgcagg agcacggcgg cactggcatg
      121 atgagacttg agaaacgagg cggcctgcga cggatgacag cgggcgcagt ctttcgggct
      181 cacgatggcg ctcaccgtga atccataatg gtcgaccatg gcatcggggt caccatcggc
      241 cgcctgatga cactcgtaac aaccgactcc gtcagccgca tgacgcccct tcttccagga
      301 ggacacaagg ttgggctgat ttaccgagtg gcaggccaga caggacgccg tcggcgtggt
      361 aaccggtgca ctcgccgtgg ccgcgagcgc gacggcggct accgccagaa acagcgatag
      421 caccaaggtt cgcaatacaa ccgagcgaaa ggcggaaagc accggcatga tcttacctcg
      481 caccgggtgt gaccggaacc gcggacgatg ccgggctgac gggtgtgggt gtgggtgcgg
      541 catctgacgc cggctggggc gtcgggttcg gcagagcttg tgagccagag ccgctgtcag
      601 ccggcggata ctcgaccggc ttgatttcgc cctcgcggta tccgggcggt aacacgcctt
      661 cgctcttcgg ctcgggtatc tgctcatacc agaagcgcac gctttcctga atcgcttttt
      721 taacctcttc gggcttcgag atacgatcgc gcagccactg atgttcgatc ttgtcgagca
      781 cgggtttggt gattccgggc atgagtcgct cggcttcggg caggaactcg aagtagaaga
      841 tcagagccac atcgtagaac ccctgccact gcacgtagtc cgcgcccatc atcgcgacgc
      901 cgtgtctcgc gcgccgcccc tgatggtgcc acagatgcca ccagtctcgc tcgagcttgt
      961 cgttcaccgg cgattcatcg agctttccgg cggctttcaa cgtatcaaga atcttcttgg
     1021 ccggcagcgc gaatttgtga ttgtacaagg ccaccgcctt ctcgagctgg atgaaatggt
     1081 tcttgacgta ggtcggactg tgacagcgat tgcagactcc ttccatcgcc tgacgtttct
     1141 cgcgccagtt gtcgcgccga acgctgatcg catcacgcag gttccaggaa aggcgatgcg
     1201 ccgggtcatg tgacgaaacc gaccccgagg cgttggtcgc gccaaagtga caggtataac
     1261 aggacggagc gccgatatga tccttgccga gtgtcagttt cggcgcattg agatcgacgc
     1321 cgcggttcaa ctcgtcgtag gcgttgccgt gttttgactc gagccagatc tcgatctgcg
     1381 gatggtcggg ccccatatga caccgaccac aaacttcggc ccggcgggcc gcgctcatct
     1441 tgaaatcgtg ccgttgatga caggccacgc agttgccttc cgagccatcg gggttcaatc
     1501 gaccgatacc gtggttgggg tacccattgg ccagaggctt tctcgtctcg ggatcaatgt
     1561 gcactttcga accgtgacac gactggcagc cgttcagtga cgccgcatac ccttcgacga
     1621 cctcgcccca gaagttcgac agcgagtgca gcacccgacc gcccttggcg tgatgcgagt
     1681 tgagaaacga gtcggcctgc tctttgtggc agcgggcgca gtcgaacgga gtcacgagcg
     1741 tactgatggt aaacccatgg tgatcgggaa tagcgtcgat atcggtcgcg ctggccacgt
     1801 ggcactcgta gcacccgacc gctttttccg catgccgtcc gcgttcccag ctcatgcaga
     1861 ggttgggatt catctgcgtg tgacaggcta gacacttctg cgtttcggtg gtggccacgg
     1921 cacgcgtggt gtacagccct tttgcgtccg ccacggccat cgccggtgtc gtcaacccca
     1981 cccagagaat cgccattacc aagtgccgcg ctcgcatatc tcgctccttc cgctctcggt
     2041 cgtcttcgtc tcttgcttcg tcttcgacgt cagaacttgt cgtaatagat atcctggctc
     2101 tgaatgccct tctgcttcag cagcggcaac agcgcatcga tcatcaccgg cggcccgcac
     2161 agataggcct cggcaggctc gccttcggga aacactttgg aaaacacggt cgtgatgaac
     2221 ccgcggtgcc cctgccaatc ggccccgaca ttgtcgtctg acagggcggg cacgtagatg
     2281 aagttcggat tcgctttcgc cagatcgacg aaatcctgat ggtcatagag cagaggaaga
     2341 gtccgcgccc cgtagaacag atagactttg cgctgaatgc gcttttccgt gatatagcga
     2401 atgatcgatt tcatcggcgc cagaccgacg ccgccggcca cgcagacaac gctgcggtcg
     2461 gttttttcct gcagaaagaa gtcgccgtag gggcccgaca ttctgagctg atcacccacc
     2521 ttgaggctgt gcagatacgg gctcatcatg cccccttcga ccaggcgcac gttgagttcg
     2581 atcacgtcgg gtcgcagcgg ggaattggcc atcgagtagc cgcgatagag cggttcgtcg
     2641 tctggcatct cgcgacacgc ctgcacgtac tgaccgggct tgaaggtgat ctgtttcggt
     2701 tcgagcaggc gaaacgtcag cttcttgatc aggtcggtca tgacttcgac ctgcgcgagt
     2761 tcgacctggt attccttgat cgccagatac tcttcgggaa cctcgatgac gagatcgcgc
     2821 cgcacgcgca cctgacaggc cagccgcttc tgctcgcgca cttcctgcgg gctgagcacc
     2881 atctgttcgg tcgccagcac cgacccgccc ccttcatgca ctttgacccg gcagtagccg
     2941 caggtgccct tgccaccgca ggccgacgga atgaacacct tgttcatcac caggctcatg
     3001 agcagagaat tgccgccctg aacggtgatc gtcttctcac cattgatgac aatcttcgcc
     3061 tcgccgtaac tgctcagcca ggcgcgcgac gcccacaaca gcaggcacag gccgagaatc
     3121 agcccgttca tcgtgagcag cgacagcagc agggttgtca tgtcagagtt tcaccattcc
     3181 cgagaatccg gaaaaagcca tggccataag cccggcgatc agcagtgcca ttccgacgtc
     3241 gccgagctgg cgtaccggtc gagcgaagat gagatgctga cgaatcgcgg ctaacaacac
     3301 gatggccaac aaccagccaa cacccgtgcc gaacgagaag accgtcgttt cccagaaccc
     3361 gtactggcga aagaccatga acagcgaggc ggcaagcacc gtacagttca cggtgatcaa
     3421 aggcagatag atgccaaacg cctcatacaa cccagggaag aagcggtcca tgagcagttc
     3481 gagaaactgg acaatcgcgg caatgacgat gatgaacacg agaaactgga agaactcgag
     3541 cccgagcggg gccagaatga agtagttgat gagccagttg accatggcgg tcagcgcagt
     3601 gacgaaggtg accgcaacgc ccatgccgaa cgcgacgctg acattacgcg acagagccag
     3661 aaaggcgcac atgccgatga agtaggccag cgcaatgttg ttggtgagac acgacgcaaa
     3721 aaagatgacc agcagccgaa tcgggtcacc cagagaaaag acgccgtagt cggcaaccgg
     3781 cgca
//
LOCUS       DAORHD010000344         3767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26770_length_3767_cov_3.024784, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000344 DAORHD010000000
VERSION     DAORHD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26770_length_3767_cov_3.024784"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1392)
                     /locus_tag="PKO06_08440"
     CDS             complement(67..1392)
                     /locus_tag="PKO06_08440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNV69710.1"
                     /translation="MRRGRWLFLIPCLADSISLLLAGALAYLLRTSSLFVVDPYLLNR
                     TEYLKLGLLAVVLWHLLLLVRGGYSLRLHLFRIDELLMQFKTSMILFVLLMASTFLYH
                     QYDYSRLILFLWWLFFVFLGSFGRQISHRLREAAFRRGWGRRRALFHGMGPKRELFET
                     RVRENPSLGIELVAAQDGRSLEEEILTQDLDELFVFDDRIEYERVWAWREKAPNPLLT
                     VHLVPSFANLYLRNFRGGFFDGAVMITLTSPASRGLELALKRLIDLAASGVFLLVLSP
                     LFALLSLIVRLDSRGPVFFRQTRIGQNGQPFTILKFRSMYEHADAYAQTPTETTDPRI
                     TRVGRLLRSTGLDELPQLWNVFCGDMSLVGPRPEMPFIVETYSALERKRLKMRPGITG
                     LWQVYARSSLLPIHSHIEYDLYYIENFSLFLDFMILLDTLPTMVLRTGV"
     gene            complement(1404..2483)
                     /locus_tag="PKO06_08445"
     CDS             complement(1404..2483)
                     /locus_tag="PKO06_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69711.1"
                     /translation="MNDKITLPPQVQEEISLYLAGDEEILEAIPSTTGSVGKLGELWM
                     LLTDRNVLFYTCEHNKDPVVALIGRKDLQSISYEIHPSGVTLTFVSMHRPQHPTRIAF
                     LRAQRPLVNRFCEKLSKDVAFEVIGADEKPEAKTTTSPRVSGMRPVMEETGAAATKPG
                     AAVASVTPTAPSGDKPSAPVSEPNVSVEIWSGDLGKGVPSGTPEHPAGATNKAVSERV
                     VEVIPPPPSRPPEVRIATSITAKPAQSQGTLKTGLGGTTTKAGEGKPPVLPASERQEP
                     ARPKVADRPAQTQAETSHKPSAESMARVTRPTEAPRVGTPTATAAETNFSEVRFVVFS
                     TLIALVVGFIWYRLFEAIGDTRPRR"
     gene            complement(2530..>3767)
                     /locus_tag="PKO06_08450"
     CDS             complement(2530..>3767)
                     /locus_tag="PKO06_08450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69712.1"
                     /translation="TTQGERVAVKLSEQLPAGKETDMSSESRIDGQPGEAPLLVMLPY
                     ASMSGLELFGVRFCLDLQSRGIPVVLAAPRDSLLHRTCLERQVNWVEMPVGPRLDPLA
                     LPRIYRLLRTLKPCAAVGFRTHVMYPFHLARLLTGQRCPLFLFYRLGAGNYQRRDPLH
                     RVLFKHLAAVIPNSDHVKNKILAKWAIAPEKVVCIKSGIDTRKYRPDAERRSAFRKSV
                     GVPDEAFLIGSTGRIHPEKGSEDLLRALYGPGGAAVHRPDVHLVYVGREYQAGYADRL
                     KAMARRFDAETRFHLAPFRAEVEAVYPAFDLFTLAVTSHETYAYVVLEAMACGVPPLI
                     PATGGLKEMVRPGVDGFLYPHRDQEALRRELATVMQHPARLEKIGRAARAWIEREADW
                     DQMMERYLAVFRQQRVEIG"
BASE COUNT          852 a         1150 c         1052 g          713 t
ORIGIN      
        1 ctcggtcggc gcttatgccg aacacgccgt caacgcccca atgctcagac tggtgaacgt
       61 cgccgattag acgccggtgc gcagcaccat ggtcggcagc gtatcgagca gaatcatgaa
      121 gtcgagaaac agagaaaagt tctcgatata gtacaagtca tactcaatgt gagaatggat
      181 cggcagcaaa ctggatcggg cataaacctg ccagagtccg gtgataccag gtcgcatctt
      241 caggcgtttc cgctccaggg ccgaatacgt ctcgacgata aacggcatct cgggacgcgg
      301 accgaccagg ctcatgtcgc cgcagaacac gttccataac tgcggcagct cgtccagtcc
      361 ggtcgatcgc agcaggcgac cgacgcgggt gattcgcggg tcggtggttt cagtcggtgt
      421 ctgcgcatac gcgtccgcgt gctcgtacat actgcgaaac ttgagaatcg tgaacggttg
      481 tccattctgc ccgatgcggg tctggcgaaa aaacaccgga cctcgagagt ccagtctcac
      541 gattaggctg agcagcgcaa acaacggaga tagcacgaga agaaatacac ctgaggcggc
      601 caggtcgatc aaacgtttga gtgccagttc gagaccgcgc gaagcgggtg atgtcaacgt
      661 gatcatcacc gcgccgtcga aaaaaccgcc acgaaaattt ctgagataga gattcgcgaa
      721 cgacggcacc aaatgcactg tcagcagcgg attcggagcc ttctcgcgcc aggcccagac
      781 acgttcgtat tcgatgcgat catcgaacac gaacagttcg tccagatcct gggtcagaat
      841 ctcttcttcg agcgatcgcc cgtcttgtgc ggccaccagc tcaatgccca ggctcgggtt
      901 ctcgcgcacc cgcgtttcga acaactcacg ctttgggccc atgccgtgaa acaaggcgcg
      961 acgtcgcccc cagcctcggc gaaacgccgc ttcgcggagc cgatgcgaga tctggcgtcc
     1021 gaaactcccc aggaacacaa agaacagcca ccacaaaaac agaatcagac gtgaataatc
     1081 atactggtgg tagagaaacg tgctcgccat cagtagcacg aacagaatca tcgatgtctt
     1141 gaactgcatc agcagttcat cgatgcgaaa cagatgcagt cgcagcgaat acccgccccg
     1201 taccagcagc agcagatgcc acaggacgac ggccaataac ccgagtttca ggtattcggt
     1261 gcgattcagc agatacgggt cgaccacgaa caatgatgag gtgcgcagga gataggccag
     1321 cgctccggcg agcagcaggc tgatactgtc agcgagacag ggaatgagga acagccagcg
     1381 gcctctgcgc atcgccactc acttcatcgc cgcggtcgcg tgtcgccgat ggcctcgaac
     1441 aggcgatacc agatgaaccc gaccaccaga gcaatgagcg tggaaaagac gacaaaacgc
     1501 acttccgaaa aattggtttc ggccgctgtt gccgtgggcg tccctacgcg gggggcctcg
     1561 gtcggacgcg tgactcgtgc cattgactca gccgacggtt tatgagaagt ctcggcttgc
     1621 gtctgcgcag ggcgatcagc gactttcggc ctcgccggct cttgccgttc actcgcaggg
     1681 agcaccggcg gctttccttc tcccgccttc gtcgtcgttc caccaagccc ggtcttcagt
     1741 gttccctggc tctgagcagg cttcgctgtg atgctggtcg cgatacgcac ttctggcggc
     1801 cgactcggcg gcggcggaat gacttcgaca acacgttcag aaacagcttt gttcgtagcc
     1861 cccgcggggt gctcgggagt accgctgggg acgcctttcc ccagatcgcc gctccagatt
     1921 tcaaccgaga cgttcggttc agagacaggg gcagagggtt tgtcgccgct cggtgccgtc
     1981 ggcgtcactg acgcgaccgc ggctccgggt ttcgtcgccg ccgcccccgt ttcctccatg
     2041 accggacgca tgccactcac gcgcggactt gtcgtcgtct tcgcttcagg cttctcgtcg
     2101 gcgccaatga cctcgaaagc aacatctttc gagagtttct cgcaaaaccg gttcacgaga
     2161 ggacgctgag cacgcagaaa cgcaattcgg gtcgggtgct gcggtcgatg catggaaacg
     2221 aaggtcagtg tcacccccga ggggtgaatc tcatagctga tactctgcag atctttgcgc
     2281 ccaatcaagg cgaccaccgg atctttgttg tgctcgcagg tgtaaaacaa cacgttgcga
     2341 tcggtcagca gcatccagag ttcgccgagc ttgcccaccg aaccggtcgt cgagggaatc
     2401 gcctcgagaa tttcttcatc accggccaaa tacagggaaa tttcctcctg cacttgtgga
     2461 ggcaacgtga tcttgtcgtt catagtgcga tactctactc cattctctcc agcaaggcaa
     2521 acccttgtct cagccgatct caaccctctg ctgcctgaag acggcaagat accgttccat
     2581 catctgatcc cagtctgcct cgcgttcgat ccaggcgcga gccgcccgtc cgatcttttc
     2641 gaggcgggcc gggtgttgca tcacggtcgc cagctctcga cgaagcgctt cctgatcgcg
     2701 atgcggatag agaaacccat caacgccggg tcgcaccatt tccttgagac cgccggtcgc
     2761 gggaatgagg ggtggcacac cgcacgccat ggcttcaagc accacgtatg cataggtctc
     2821 gtgcgaggtc acagccagag taaacagatc gaatgccgga tacaccgcct caacttcagc
     2881 ccgaaatggt gccaggtgaa agcgtgtctc tgcgtcgaaa cgacgcgcca tggccttgag
     2941 gcgatccgca tagcccgcct ggtattccct gccgacatag accagatgaa cgtcaggccg
     3001 atgaaccgcg gcaccacccg gaccatagag agcccgcaaa aggtcttcag aacctttttc
     3061 cgggtgaatg cgaccagtac tgccaatcag aaaagcctcg tccggaacac ccaccgactt
     3121 tcggaaagcg cttcgccgct cggcatccgg tcgatacttc cgggtatcaa tgccactctt
     3181 gatgcagaca accttttcgg gagcgatcgc ccacttggcc agaatcttgt tcttgacatg
     3241 atctgagttc ggaatgacag ccgccagatg tttgaacaac acccgatgca gcggatctcg
     3301 tcgctgatag ttgccggctc ccagtcgata gaagagaaac agcggacaac gctgaccggt
     3361 caacagacgc gccagatgaa acggatacat gacatgcgta cgaaatccta ctgcggcaca
     3421 gggcttcagg gtgcgcaaaa gacgatagat gcgaggcaag gccaacgggt caagtcgcgg
     3481 gccaaccggc atctcgaccc agttcacctg acgctccagg cacgtgcggt ggagcagact
     3541 gtctcgcgga gccgcaagca cgacgggaat gccacgtgac tgcagatcga gacagaagcg
     3601 caccccgaac agttccaaac ctgacatgct ggcatacgga agcatgacca gaagcggcgc
     3661 ttcacccggc tgtccatcaa ttcggctctc gctcgacata tctgtttcct ttccggccgg
     3721 aagctgctca gacaacttca ctgccacacg ctctccctgt gtcgtgt
//
LOCUS       DAORHD010000345         3762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26815_length_3762_cov_3.308875, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000345 DAORHD010000000
VERSION     DAORHD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26815_length_3762_cov_3.308875"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1275)
                     /locus_tag="PKO06_08455"
     CDS             complement(310..1275)
                     /locus_tag="PKO06_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69713.1"
                     /translation="MAKDQPTARAEPSKPATAPAGINNPPVPGVATPGSEATSTESTE
                     APAAIPPILSKIYRVISAIAFFGLLVTFFAVMTVFLFTLDFLDLFQWRYKVPDAWRKK
                     WPLERYYDFVKLYQLPEAERYYALQKQNKEIYDQLIASGSSDLRRRADALESAYRDQV
                     QKQEESYKKRLEELRVIQEDNLKMKARLEQMEKDLQARREAVDVMSRQVASEAVNIES
                     SLIRFMEDENRLKPVQEMAASMDPRSVAAIFDEVSDNKLLYDILKGIPPERSALVLSY
                     MDPEKAGKIVKMSQMPPTLPAPGSPRSYLPSSLQGLIASSQSVLR"
     gene            complement(1283..1921)
                     /locus_tag="PKO06_08460"
     CDS             complement(1283..1921)
                     /locus_tag="PKO06_08460"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV69714.1"
                     /translation="MLEKMREIQDRIEGIKDRCRELRGQRKPQLPKETFNKVLQEAGL
                     PTTTPQEPPAKATALPRLPPNGTTAPIELRAPAQKPNDVFSSLIDKYARLHNLDPKLV
                     RRLIEVESDFDPACISSKGAMGLMQLMPETAQELGVENPFDPEQNISGGTRYLAQMLE
                     REGGNLARALAAYNAGPTTVRDYNGIPPYPETRNYIRKILGKLPGDDASEKD"
     gene            complement(1914..2366)
                     /gene="fliJ"
                     /locus_tag="PKO06_08465"
     CDS             complement(1914..2366)
                     /gene="fliJ"
                     /locus_tag="PKO06_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNV69715.1"
                     /translation="MPFSYRFHHLLRLAEHEEEEVRRRLAMKDGQIAAAEAEIRKIQL
                     ERQHGLEAKAVDLIAGAMERVRLYPAYFLRLQNREDFQNEELERLRKQREKIAQELAE
                     KRQTRKTYEKIRERDEAVFKKQEQRRDQKNMDEFASRLTRPFAEENHA"
     gene            complement(2374..3684)
                     /gene="fliI"
                     /locus_tag="PKO06_08470"
     CDS             complement(2374..3684)
                     /gene="fliI"
                     /locus_tag="PKO06_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HNV69716.1"
                     /translation="MVNFPFGQYLNAIEETDPLRQHGRVTQVVGLLIEATGPQMPVGE
                     VCHVKTQTNLVPCEVVGFRRGSVLLMPLIHLEGVRQGSEVIPVGAPLTAPVGDELKGR
                     LLDGLGNPADERGELPASVKRVSIYRDPPNPFARKRITGILSTGVKVLDGMLTIAPGQ
                     RLGIFAGSGVGKSTLLGMIARTSTADVNVIGLVGERGREVREFIEKDLGPEGLRRSVV
                     VVATSDRPALLRVKAAFTATAIAEYFRDQGKNVMLMMDSVTRFAMAQREIGLAVGEPP
                     TTRGYTPSVFSMLPKLLERAGTARQGSITAIYSVLVEGGDMDEPIADAVRGILDGHLV
                     LSRQMAEQNLYPAIDILGSISRLMNDVASKRHLEVAARVRDLYSTYTQSADLINIGAY
                     KQGSNPKIDESIAKLPRIQNFIRQGIHDSVPFEETLKQLEAIIQ"
BASE COUNT          727 a         1041 c         1149 g          845 t
ORIGIN      
        1 cagtgaatcc gtgaacgctg ccgaaccctc tgccgtcgaa aaagcgttgc agcgcctgcg
       61 tgagtcgcga ctggcccgtc agacgcgatt ggcgaaagcg gatctcccag gagccggcga
      121 gaaaaagtaa ctcttctgag taggaaaagg gccgcgggtc aggtgaacga ctcgcggccc
      181 tttcgcatga gcgtgagacc gaacttggtc gtggctggcg atggttgcgt gacgaaagaa
      241 aacagcgaaa ccagtcactc agcgagagaa atcgttggtg gtcactcgga cgaacgtcgc
      301 aggtgaatct cagcgaagaa ccgattggct tgaggcaatc agtccctgca ggcttgaagg
      361 aagatagctg cgcgggctcc ccggagccgg gagagtcggc ggcatctgag acattttcac
      421 gattttgcct gccttctctg gatccatgta gctcagaacg agggcggaac gttcgggtgg
      481 gatccctttc agaatgtcat agaggagctt gttgtcagag acttcgtcaa agatggcggc
      541 gaccgatcgc gggtccatag aggcggccat ttcctgtacc ggtttcaggc ggttttcgtc
      601 ctccatgaaa cggatcagac tcgactcgat gttcaccgct tctgacgcga cttgccggga
      661 catcacatcc acggcttccc tgcgtgcctg aagatccttt tccatctgct cgagtcgggc
      721 tttcatcttc agattgtctt cctgaatgac tcgcagttcc tcgagacgtt tcttatatga
      781 ttcttcctgc ttctgcacct ggtcacgata ggctgattcg agggcatcag cccgtcggcg
      841 caggtcagaa ctgccgctcg caatcaactg gtcgtagatc tccttgttct gcttctgcag
      901 agcatagtaa cgttcggcct caggaagttg atacaattta acaaagtcgt agtagcgctc
      961 gagaggccat ttttttcgcc aggcatctgg tactttataa cgccactgaa acaggtcgag
     1021 aaaatcgagc gtaaagagaa aaacggtcat gaccgcaaaa aaggtcacta acaacccgaa
     1081 aaaggcgatg gcgctgatca cccgatagat cttcgacaga atgggcggaa tcgcggcggg
     1141 cgcctcggtc gactcggtcg aagtcgcttc acttcccggg gtggcaaccc cgggtaccgg
     1201 tgggttgttg attccggccg gtgctgttgc cggtttgctg ggttcagctc tcgcggtcgg
     1261 ttggtctttc gccatgaatc actcaatcct tttcggaggc atcatccccc ggcagcttgc
     1321 cgagaatttt ccgaatgtag ttgcgggttt ccggatacgg ggggatcccg ttgtagtcgc
     1381 gcacggtggt tgggccggcg ttataggccg caagcgcgcg cgcgaggttg ccgccttcgc
     1441 gctccagcat ctgggccaga tagcgggttc cgcccgagat gttctgctca gggtcgaaag
     1501 ggttctccac gccgagttcc tgcgcggttt cgggcatgag ttgcatcagg cccatggccc
     1561 ccttgctgct gatacaggcc ggatcaaaat cggactcgac ctcgatcaag cggcgcacga
     1621 gcttggggtc gagattgtga agtcgagcgt acttgtcgat aagagacgag aatacgtcgt
     1681 tgggcttctg ggccggagcg cgaagttcga tcggcgcggt cgttccgttg ggcggcagac
     1741 gcggcagagc cgtcgctttg gcgggtggtt cctggggagt tgtggtcggc agaccggctt
     1801 cctgcaagac cttgttgaag gtttctttgg gcagctgggg tttgcgttga ccacgcagtt
     1861 cgcggcagcg gtccttgatg ccttcgattc gatcctgaat ctcgcgcatt ttctcaagca
     1921 tggttttcct ccgcgaaggg gcgcgtcagt ctcgaggcaa actcgtccat gtttttctga
     1981 tctctgcgct gttcctgctt cttgaacacg gcttcatcac gttcgcggat cttctcgtag
     2041 gtttttcgag tctgccgttt ctctgccagt tcctgggcga tcttttcgcg ctgtttgcgc
     2101 agacgttcca attcttcgtt ctgaaagtct tctcggttct gcaggcgcag aaagtaggcg
     2161 gggtacaggc gcacgcgctc catggctccg gcaatcagat caaccgcctt ggcttcaagc
     2221 ccatgttgcc gctcgagctg aatctttctg atttcggcct cggctgccgc gatctgaccg
     2281 tctttcatgg ccaaccgacg tcgcacctct tcctcttcgt gttcggcgag gcgcaacaga
     2341 tgatgaaaac ggtaggaaaa cggcatattg aacctactgg atgatggctt caagctgttt
     2401 cagggtttcc tcgaaaggta cggaatcgtg aatgccctga cggatgaagt tctgaatgcg
     2461 ggggagcttg gcgatggatt catcaatttt cgggttcgac ccctgcttgt aagctccgat
     2521 gttgatcaga tcggcggact gggtatacgt ggagtacagg tcgcgaactc gcgccgcgac
     2581 ttccagatga cgctttgagg caacgtcgtt catgagacgc gagatgctgc cgagaatgtc
     2641 gatggcgggg tagagattct gctcggccat ctgtcggctc agtaccaggt gaccatcgag
     2701 aataccgcga acggcatcgg cgatcggctc atccatatcg ccaccttcga ccagaaccga
     2761 atagatggcc gtgatcgaac cctgacgggc ggtaccggcg cgttcgagta acttcggcag
     2821 catcgaaaag accgagggcg tgtacccgcg cgtggtcggg ggttcaccga cggcgagacc
     2881 gatctcgcgc tgagccatgg cgaaacgggt cacggaatcc atcatcagca tgacgttctt
     2941 tccctgatcg cggaaatact cggcgatggc ggtggcggtg aacgcggcct tgacgcgaag
     3001 cagcgccgga cgatcggatg tggcgaccac cacgacggag cggcgcaagc cttccgggcc
     3061 gagatctttc tcgatgaatt cgcgcacttc gcggcctcgt tctcccacca gaccgatcac
     3121 gttcacgtca gcggttgacg tgcgggcgat catgccgagc aacgtgctct tgccaacccc
     3181 cgaaccggca aagatgccca gacgttgtcc gggggcgatg gtcagcatgc catcgagaac
     3241 tttgactccc gtggacagaa taccggtgat gcgcttgcgg gcaaatgggt tgggggggtc
     3301 acgatagatc gagacccgct tcaccgaagc cggcagttct cctcgttcgt cggccgggtt
     3361 gccgagaccg tcgagcaggc gccccttgag ctcgtcaccg accggagcgg tcagcggtgc
     3421 cccgacggga atgacctcac tgccttgtcg aactccttcc aggtggatga ggggcatcag
     3481 caacacggag ccgcgtcgga accccaccac ttcacagggg acgaggttcg tctgggtttt
     3541 gacatggcag acttcgccaa cgggcatctg cgggccggtg gcttcgatga gcaggccgac
     3601 cacctgcgtg acacgtccgt gctgacgcag ggggtcggtc tcttcgatgg cgttcagata
     3661 ctgaccgaac ggaaagttca ccatgattta ctctggacga tcgaggattt tcagcagagc
     3721 tttctcgagc tcgtccgcct gggcttcgat cgaggcatcg at
//
LOCUS       DAORHD010000346         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26864_length_3757_cov_2.693679, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000346 DAORHD010000000
VERSION     DAORHD010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26864_length_3757_cov_2.693679"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1322)
                     /locus_tag="PKO06_08475"
     CDS             complement(219..1322)
                     /locus_tag="PKO06_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV69717.1"
                     /translation="MDKLFRRLKNFLPLLSYQFGTARISPFPNVVYVSVNNVCNANCV
                     MCDIGLKNKNSSFYQNLIQNGRSELSLELLRKLLSGVRKYRPIIHINSTEPLLYRNLI
                     AAIQLVKRAGLELWLTTNGILLEKWAEELVTSGVDRIFVSIDGTEEVHDKIRGSGCFA
                     KAAAGIRAVRLQREKARSMVPTIQVNTTISNLNGKNLFQFARYAIEELCVDHLTFSHM
                     NFVTEEMAACHNRLYGTLGTATMASIKVLNPSDVDVESLHENLRLIRESDSASSISYV
                     PHLASLEEVRKYYTDPSFIVSRKKCLVPWRNATLQPNGEMIVLHRCFHYCVGNLYEHS
                     FQQIWNGQRYRTFRRSLKSAGLFPVCSRCCGAL"
     gene            complement(1379..2527)
                     /locus_tag="PKO06_08480"
     CDS             complement(1379..2527)
                     /locus_tag="PKO06_08480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69718.1"
                     /translation="MRKSILFICPDYHCTFFYRDELRKLGWKADILVGNSYPEKLLYS
                     EDGIIRVPGLAGGGPMARIGNALLNRLYFFSIFWFYQFLFIYDEIDQLHCGERFLGLT
                     RIFGKSFRVSLLLAKLLGKKIIFMPAGCNGEESKASFSKLDEGEVCRNCGWGPETCDD
                     ERNHDRFRLCRKYVDLVVGNGSLDSSQFKTTHFKYKAIDLNLWRPDLEIPPEFRLKPT
                     ENLRILHSFYNLNREHDGRNIKGSPHILRAIERLRQEGHKVEYMFINNVPSRNMRYYQ
                     AQADIVVDQLIYGWWGSTLVETTALGKPVICYLRPAWKQFFLQTFPEYSGLPVIEADR
                     ASIYDVLKNLVVDAHYRQAAGVKSRRFAEQHFDVSQNARGLISLLKGL"
     gene            complement(2533..>3757)
                     /locus_tag="PKO06_08485"
     CDS             complement(2533..>3757)
                     /locus_tag="PKO06_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HNV69719.1"
                     /translation="GKRPAIIWTTATILFLHALVLTTIFWFLASKLSEAMFQTAEHMP
                     LFRIAFVGLFLTTLNEPFYSYLRMDEQAKTFVRVTLLGSFLTAGLSAVFVLILGWGTE
                     GYMLAPVISQLVILVILLTYMLRQLPYSWNAQYGIPLVKIGFPSIFGLFAFMIIDYAD
                     RQLIQRFLGLEQLGLYSLGYSFGMIMLVLVNAFASAWPPFFMSFIGRQDEAKVLFGKV
                     LKAYLALFSIACVGFFALARPVLELLTASSFHGAHAVVGIIAASYLLRGCYLIFLPGV
                     YFSQKLYVQSVIEWLAAGVNILLNLQLIASYGIVGAALATLASYTILPVTTWIVSRRH
                     LAVNYDWNAIVRIVGACVASCLILHRLSRDTSLTLFMLLSFLVFASYVLWVLGGCLTS
                     EERHSVVKGKLLVSS"
BASE COUNT         1095 a          948 c          883 g          831 t
ORIGIN      
        1 gaaagagaca gggtaatcca ccgacttcga caccaaagcc gtgacgagga gcgcgccccc
       61 caacatgacg atcatcagta tgggtggaaa caccagcgca agaagcgcca tcagacacaa
      121 ggctgcacta ccccatacca atttcctgaa gcgagcggtg tttgtgtaat aactccaaac
      181 cgcgctcacg taggtgcgaa atgacacgga cacacctttc acaatgcgcc acagcaacgg
      241 ctacagacgg gaaacaggcc ggcagacttc agtgaccgac gaaacgtgcg atacctctgt
      301 ccgttccaga tttgctgaaa gctgtgttca tacaagttcc cgacgcaata atgaaaacag
      361 cgatgcagaa cgatcatctc gccgtttggc tgcagcgttg cattgcgcca gggaacgaga
      421 catttcttcc gtgacacaat gaatgatgga tcagtatagt attttctgac ttcttcgagg
      481 cttgccaaat gcggcacata agaaatggat gaagcagagt cactttcacg aatcaggcgc
      541 agattctcat gcaggctttc cacatcaacg tcactcggat tcaagacctt gatgcttgcc
      601 atcgtcgcag ttccgagtgt cccatacaaa cgattgtgac aagcagccat ctcctccgtg
      661 acaaagttca tgtgcgaaaa agtaaggtgg tcaacgcaga gttcctcgat ggcgtatcgt
      721 gcgaattgaa acaagttctt gccattcaga ttcgagatag tggtgttcac ctgtattgtc
      781 ggcaccatcg atcgagcttt ctctctctgc aagcgaacgg ctcgaatccc cgcggcggcc
      841 tttgcaaaac agccactccc ccgaatcttg tcgtgaactt cttccgttcc atcaattgaa
      901 acaaagattc gatcgacccc tgatgtcacc aactcttcgg cccatttctc cagaagaata
      961 ccatttgtcg tcaaccagag ttcaagtcct gcacgcttca ccagttgaat cgcagcgata
     1021 agattacgat acaagagagg ttcggtggaa ttgatgtgaa taatcggacg atactttctc
     1081 actccggaaa gcagtttacg aagcaattcc aaactcaatt cactgcggcc attctggatc
     1141 agattctggt aaaatgaaga attcttattc ttcagtccga tgtcgcacat gacacagttc
     1201 gcattacata cgttgttcac agatacatag acgacatttg gaaacggact gattctcgcc
     1261 gtaccgaatt ggtaggagag aaggggcagg aagtttttca atcgtcgaaa gagcttatcc
     1321 atgagaccaa acatgctttg caacaagaaa gtcatagacg aatcagatgg cattttcttc
     1381 acaaaccctt caagagcgaa atcagccccc tggcattctg gctaacatca aaatgctgtt
     1441 cagcaaatct tctcgacttc acaccagccg cttgtctgta gtgggcatcg accaccagat
     1501 ttttcaagac atcgtaaata ctcgctctgt cagcctcaat gaccgggaga ccagagtatt
     1561 caggaaaggt ctgcaagaaa aactgtttcc aggcaggtcg caagtaacag atcaccggct
     1621 tgccaagggc tgtcgtttct accagtgtcg aaccccacca accatagatc aattgatcaa
     1681 ccacgatatc cgcctgtgcc tgataatatc tcatgttcct gctcggaaca ttgttaataa
     1741 acatgtattc gaccttgtgt ccttcttgtc gcaagcgttc tatcgctctc agaatatgtg
     1801 gagatccctt aatgttcctg ccgtcgtgtt cgcgattcag attataaaac gaatgcagaa
     1861 tgcgtagatt ttctgtgggt ttcaatcgaa attccggcgg tatctccagg tctggtcgcc
     1921 acaaattcag gtcgattgct ttgtatttga agtgagttgt cttgaattgg gatgaatcga
     1981 gcgagccgtt tccgacgaca aggtcaacat attttcggca gagtctgaag cgatcatggt
     2041 tgcgctcgtc atcacatgtt tccggacccc atccacagtt acggcacact tcaccctcat
     2101 ccagttttga aaaactcgct tttgattctt ccccattaca cccggccggc atgaatatga
     2161 tcttcttccc caggagtttg gccagcaaca atgacacgcg aaaactcttt ccgaagatgc
     2221 gcgtcaggcc gagaaatcgc tcaccacaat gcaattgatc aatctcgtca taaatgaaaa
     2281 gaaattgata gaaccagaaa atcgagaaga aatacaaccg gttcagcaac gcattgccaa
     2341 tgcgtgccat cggtcccccg cccgcaagtc caggaacgcg aataatgccg tcttcggaat
     2401 atagcagttt ttcagggtac gagttaccga ccaggatgtc ggctttccag ccaagcttgc
     2461 gcaattcatc gcggtagaag aaggtgcagt gatagtccgg acagatgaag agaatggatt
     2521 tacgcacggt tatcacgaag aaacgagaag ttttcccttg acgacagaat ggcgttcctc
     2581 agacgtgaga caaccaccca gaacccacag aacataggac gcgaaaacga ggaatgatag
     2641 taacataaag agagtcagcg aggtgtctct tgaaaggcga tgcagaatca gacaagaggc
     2701 gacgcaggcg ccgacgattc tgacaatggc gttccaatcg tagttgaccg caagatgtcg
     2761 tcgagacaca atccaggtcg tcacgggtaa aatggtgtaa ctggccaggg tcgccaaagc
     2821 cgccccaaca atcccgtaag aggcaatcaa ctgcaggttc aggagaatat tcacaccagc
     2881 tgcgagccat tcgatcacgg attgtacata gagcttctga gaaaagtaca ctccaggcag
     2941 aaagattaaa tagcatcctc tcaacaaata cgaagcagcg atgatcccta cgacggcatg
     3001 agcaccgtga aaggacgatg cggtaagcag ttcgagaaca ggcctggcca gcgcaaaaaa
     3061 gcctacacag gcgatactga acaaggccag atacgctttc aacactttgc cgaacaacac
     3121 cttcgcttcg tcctgcctgc cgataaacga cataaagaat ggaggccagg ctgatgcaaa
     3181 agcattcacc agaacgagca tgatcatgcc gaaactgtat cccagcgaat agagccccag
     3241 ttgttcaagc cctaagaatc gctggataag ttgtctgtcc gcatagtcga tgatcatgaa
     3301 tgcaaacaaa ccaaagattg atgggaagcc aattttcaca agaggaatcc cgtactgagc
     3361 attccaggag tatggcagtt gtcgcagcat gtaggtgaga agaatcacaa gaatgaccag
     3421 ttgagagatg acaggagcga gcatgtagcc ctcggtgccc cacccaagga tcaagacaaa
     3481 aactgcactg aggccagcgg tgagaaaaga tccaagaaga gtaacccgaa caaatgtttt
     3541 cgcctgttca tccatgcgaa ggtatgaata gaaaggctca ttcagcgttg tgaggaacaa
     3601 ccccacgaag gcgatacgaa aaagcggcat gtgttcagcg gtctgaaaca tggcttcact
     3661 cagcttcgag gccagaaacc agaaaatggt ggtcagcacc agcgcatgaa ggaagagaat
     3721 ggttgccgtt gtccagatga tggcaggccg tttccca
//
LOCUS       DAORHD010000347         3756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26870_length_3756_cov_3.616860, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000347 DAORHD010000000
VERSION     DAORHD010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26870_length_3756_cov_3.616860"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..369)
                     /locus_tag="PKO06_08490"
     CDS             complement(100..369)
                     /locus_tag="PKO06_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNV69720.1"
                     /translation="MPIVSMFYGIVIRFYFFDTQQHHQPHFHAEYAGHKAVFSIPDGA
                     ILAGNLPVNKRKLVEAWIEIHREDLLADWNLASQGAPPVPIEPLK"
     gene            542..1153
                     /locus_tag="PKO06_08495"
     CDS             542..1153
                     /locus_tag="PKO06_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69721.1"
                     /translation="MLHARTHALTLILALFTLLVAIPAWAETPFAKIDLEPRLAPEGW
                     ENARRYEIIYLDAGGEELGEADLPFYMKKQEIDLILAGVVRQILAREYETQGFKISVH
                     QVTSSTPQEILLMAAVGSGNGKDRWAPETLTNLYQYMNNDPTHLFLYKMTAQLETEAG
                     TVEEKLPLLLRMKQCPGQRDLISFFFVEKQQVPQETVPAPVVR"
     gene            complement(1422..1949)
                     /locus_tag="PKO06_08500"
     CDS             complement(1422..1949)
                     /locus_tag="PKO06_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69722.1"
                     /translation="MFRYTPRLIALTLLTAIAVSASTDAPSSVPASDLVNTHTLTRSG
                     SSTQQTSIDVRDILILNYHDFGPQAAAWPLLGMEWHQWESHGDSDPARRYDVEVVVYR
                     DVDVRVVRKLYPVSQRESKDYRYVSFQKALRYLDDTIRENLVADVTRTLIDTRNRLLR
                     HFHASSRRSSPKRVP"
     gene            complement(2134..3228)
                     /locus_tag="PKO06_08505"
     CDS             complement(2134..3228)
                     /locus_tag="PKO06_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HNV69723.1"
                     /translation="MKRLFSLVLLLVVLLTGSAYAAQTPTVWNELFPLGLLDRDGQPV
                     ALETLQGKYVGLYFSAEWCRTCQKFSPLLVPFRNDHAAAFEVIMVSSDKNEAAQFDYM
                     KKYGMLWPTLKYQSAPAKALRERFEVTSIPMLIILSPDGKLVKIDGRTDVEGDPAGAM
                     AKWQAITPTPLPFTIESADRRPVGEKDRLAQLDRQIIDDVNTLRAIPKQGWIKGTISG
                     KSYFVTFDRTSWTIKGTAGDKPVDIKIDHENKRIQGFAHDSGVDLYFTWATDAYSLEG
                     DTYGTPYWVNISWPGAEANGGIACSMITLSWNLDTGKITGFLSDRKADMKFDKVSGRL
                     TGDFFKRPIDLQITNLELSDFITHLYLFLK"
BASE COUNT          786 a          995 c         1163 g          812 t
ORIGIN      
        1 aatgtcgaaa caacgcgttt cctgattgtc gaatgtcacc atcaattcga agtcagagat
       61 cgcgtggaca cttgtcacgt atgggttcac gattgttacc tatttgagag gttcgattgg
      121 aaccggtgga gccccttggg aggccaggtt ccagtcggca agaagatctt cacggtggat
      181 ttcgatccag gcctcgacaa gttttcgttt gttcaccgga aggtttccgg caagaatcgc
      241 gccgtcagga atggaaaaaa ccgccttgtg accggcatac tccgcgtgaa agtgaggttg
      301 gtggtgctgt tgtgtgtcga agaagtagaa acgaatcacg atgccgtaaa acatcgatac
      361 gatcggcatg ctgatgtcac tgctcctgtt tccaatgtcg aaatacacta ccacgtcact
      421 ggccgggaaa caaggccaga accagtgatg cggcgaggtc gagaccgggt ctgtacaact
      481 cgtcgatttg tgagtataat ctagacatct tgaatgtacc cgttggtggg aggaacgtga
      541 gatgttgcat gcgcgaaccc acgccctgac tttgattctg gcgcttttta ccctgctcgt
      601 ggcgataccg gcgtgggccg agacgccttt tgcgaagatc gatctcgagc cgcgtctggc
      661 acccgaaggg tgggaaaacg cccgccggta tgaaatcatc tatctcgatg ccggtggcga
      721 agaactgggt gaagcggatc tgccgttcta catgaaaaaa caggaaatcg acctgattct
      781 cgctggcgtg gtgcgccaga ttctggctcg cgagtatgaa acccaggggt tcaagatttc
      841 cgtgcatcag gtaacctcga gcaccccgca ggagatcctg ctgatggccg cggtcggctc
      901 tggaaacggc aaagatcgct gggcccctga gacgctgacc aatttgtacc agtacatgaa
      961 caacgatccg acgcacctgt ttttgtacaa gatgaccgcc cagctcgaaa ccgaagccgg
     1021 cacggtcgaa gagaagctgc cgttgctgct gcgcatgaag caatgccccg gccagcgaga
     1081 cctgatctcc ttcttcttcg ttgagaaaca gcaggtgccg caggagacag ttccagcccc
     1141 cgttgtccgc tgatcagtcc agccggggca aacggcaccg atgagtagat actgagagtg
     1201 tttcagctcc ggtgagctca ggtgagtgcc gtgtgtgtga acgccccggt ggcccgtgtc
     1261 ggtactacct ttgtcatcgc ctgcgtccac ggcgctttcc ccgatgtgcc tcaccagcct
     1321 gggtgtttac ggggtttcgc cgagcccaac ggtttcgctt cctgtttcca aacatctcgg
     1381 tcgaaagagt ttcggtgtgc gtgactgccg ggggtgctgc ttcaaggcac acgtttgggt
     1441 gaagaccgcc tcgacgaggc gtgaaagtga cgcagcaggc gatttcgagt gtcgatgagg
     1501 gtgcgcgtga cgtcggccac gaggttttcc cgaatggtgt cgtcgagata ccgcagggcc
     1561 ttctgaaatg aaacatatcg ataatccttg ctttcgcgct gactgacggg gtaaagcttc
     1621 ctgacaactc ggacatccac atctcggtag acgacgactt cgacgtcgta tcgtcgggcg
     1681 gggtctgagt cgccgtggct ttcccactgg tgccattcca tgcccaacaa aggccaggcg
     1741 gccgcctgcg ggccgaagtc atgatagttc agaatgagaa tgtcacgcac gtcaatcgac
     1801 gtttgctgtg tcgaagagcc agatcgcgta agcgtgtggg tattgacgag atcagacgca
     1861 ggaaccgagg atggcgcgtc ggtcgaggcc gaaacggcga tcgcagtgag aagtgtgaga
     1921 gcgatcagtc gcggggtgta ccgaaacatc gagaatcctc cttgagcggg gcagatcgtg
     1981 agcgaaggaa tgcgctttcg aggcgcgcgt gttgattcga tccacaaacg agatatgaga
     2041 tatgagatac tccgcggtgc cgccgaacgg cgcggagaag agaaacgagg ctgtcttgcg
     2101 acagcctcgt cagggcttct gaacgcggtt gagctacttc aggaacaggt acaggtgcgt
     2161 gatgaagtcg ctgagctcga ggttggtgat ctgcagatcg atcgggcgct tgaagaagtc
     2221 gccggtcaga cggccgctga ccttgtcgaa cttcatgtcg gctttgcggt ctgagagaaa
     2281 gccggtgatc ttgccggtgt cgaggttcca cgaaagggtg atcatcgagc aggcaatgcc
     2341 gccgttggct tcggcacccg gccagctgat gttgacccag tagggcgtgc cgtaggtgtc
     2401 gccttcgagt gagtaagcat cggtcgccca ggtgaaatac aggtcgacgc cgctgtcgtg
     2461 ggcaaatccc tgaatccgct tgttctcatg atcgatcttg atgtcgaccg gcttgtcgcc
     2521 ggcggtgccc ttgatggtcc aggacgtgcg atcgaaggtg acaaagtacg atttgccact
     2581 gatcgtccct ttgatccagc cctgttttgg aatggcgcga agggtgttga cgtcgtcgat
     2641 gatctggcgg tcaagctggg ccagacgatc tttctcgccc acggggcggc gatcggcgga
     2701 ctcgatggtg aacggcagcg gggtaggtgt gatcgcctgc cacttggcca tcgctccagc
     2761 cgggtctccc tcgacgtcgg tgcggccgtc gatcttgacc agctttccgt ctggcgagag
     2821 gatgatcagc atcggaatcg aagtcacttc aaagcgttca cgcagggctt tcgccggagc
     2881 cgactggtac ttcagggtcg gccagagcat gccgtatttc ttcatgtagt cgaactgagc
     2941 ggcttcgttc ttgtcagaac tgaccatgat gacttcgaaa gccgcggcgt gatcgttgcg
     3001 aaacggcacc agcagaggcg agaacttctg acaggtgcgg caccattcgg ccgaaaaata
     3061 gaggccgacg tacttaccct gcagagtctc gagcgcgacc ggttgtccgt cgcgatcaag
     3121 caaaccgagg gggaataact cgttccagac ggtcggggtc tgcgcggcat acgcgctgcc
     3181 ggtcagaaga acaaccaaca acagaaccag ggagaacagg cgtttcatcg ttcacctccg
     3241 ggacaaactc ttggatacca acaccgtagc agattcgaca acccgctgtc taccgaagga
     3301 cgacaccagt catgcccgtt tccgcataaa tcgtgtatcg ttggcgtcgc caggtctctg
     3361 ccgttgccct gtctcactcg ccgagcctgg tcgccatcaa aaccggaagg tgtggatgga
     3421 acggtctggc gaacggctgc tcaatgaccc atgacacggg tagagagagg gtttcggctg
     3481 tcgcgggagt tgcctgccga tggttcggca ggacagtgcc ggattttccg ctgtgccagg
     3541 gctgtcggcc cgactgcatc gcgcagcatc gctgttcgca cgttttcgct ggttggaaca
     3601 ggaattgctt tggtcccggt gatcgatttt cgaaccgggg gaaggcacat gcgcactatc
     3661 atctggggtt tgctgctggt cgcttgtatc gctgggggtt ccatgacgct ctgggccgaa
     3721 ggcttcaaga gcgtgtaccc gaacgccaag atcgag
//
LOCUS       DAORHD010000348         3751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26927_length_3751_cov_3.352543, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000348 DAORHD010000000
VERSION     DAORHD010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26927_length_3751_cov_3.352543"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKO06_08510"
     CDS             complement(<1..466)
                     /locus_tag="PKO06_08510"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNV69724.1"
                     /translation="MSSRKILVTVGPYETRAAILDNDQVTELFFETRETEKIVGCIYK
                     GRVANVVPGTQSAFVDIGLEKDAYLTLSGIEPLDGGDDGDLKDIFPTPIKDLIKVGQD
                     VLVQILKEPSPGKGPRSTMYLSLPGRYLVFMPTWDHIGVSRKISSDRERERLR"
     gene            complement(426..1166)
                     /locus_tag="PKO06_08515"
     CDS             complement(426..1166)
                     /locus_tag="PKO06_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNV69725.1"
                     /translation="MGDMKVRLTFSRHESARFISHLDAMNHLLRAVRRSGLPYQVTQG
                     CHPRPKTSFGPPVALGFASRCEVMDLTLRESVPVEEAANLVRQQLPIGFGLVSAVAVE
                     PGMPPLTAEAETEFRLEFAPDAAELRETARRFFLDPETRIRAGKPGQEREVILAGAVS
                     EARLVDGPPLALHLIFAAPAKGIPSGAKIIQAFLAGLGDRRPQITLIERVRISAAVAV
                     RQVSPNHSQTQQEVTSEFAKNSGDGGPV"
     gene            complement(1168..2919)
                     /locus_tag="PKO06_08520"
     CDS             complement(1168..2919)
                     /locus_tag="PKO06_08520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69726.1"
                     /translation="MIPGISDWELLDIEKPARYVGGEWNQPQAKANPQLTVCLAFPDL
                     YELGMSNLGLRILYDRLARDERIQVERTFSPWIDFERLLRRKGVPLFAVESKRPVRDF
                     DLLGITLPYEMTFTNVLNLIDLAGLPLLAKDRCSGPLVIGGGPSASNPVPVAEFFDAI
                     LVGDGEDAILDIADLLLQAKTRGWRREEVLERLAAIEGYFVPRFPAPVKRRVYKEFGQ
                     SAPPLKPLISNIESVHDRMPLEIFRGCIQGCRFCNAGFFYRPKRERSVNDLCGWAQQI
                     LQSTGEETLGLISLSTSDYSCLGELVDRLGREKRYPDQTLAVPSLRMNDQTLTLLERI
                     DEMKKSGLTFAPEAGSQRLRDIIHKKITEDEILHVIAATKGSNYQSIKLYFMMGLPFE
                     TEADLDAIPELVFRIEDVCRRERLRKDLTISLSGFVPKPFTPFQWCRQATPQELFERR
                     QRVCQGLRKSRARISWRDEFLCLLEGVLSRGDAQVGKVLFEAWKAGATFDGWCDRFRE
                     DAWKQAFERAAVDPTTYTREMSVDQPLPWEFIDFRTPRAYYLDEYRRATDLARVGSSS
                     VPGVVESAEPVKPLVEA"
     gene            complement(2916..3194)
                     /locus_tag="PKO06_08525"
     CDS             complement(2916..3194)
                     /locus_tag="PKO06_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69727.1"
                     /translation="MVHDSHHEDGQLTEAERGLTPVEVMKLQLMRDRLNLPPAESRRL
                     LEQLRREIAGVMGVSLDQMLADIDFSGPEMDLVFTFPESAVRGGRPSR"
     gene            complement(3194..>3751)
                     /locus_tag="PKO06_08530"
     CDS             complement(3194..>3751)
                     /locus_tag="PKO06_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69728.1"
                     /translation="YYNLGVFLMDNGRREDGIKLFHECIKHDMYHLKSRYALGKIAQD
                     AGNHQEAIRHYKAFQQRDRGDLKVHMALGETYLLAGMIESSQREFESAAKIDEKNPMI
                     HYQLGVAYHRSNRIAEAIHEYQRATELAPDMIDGFINLGDLYVHRGEHAKALRAYNAA
                     LGIDSRNAYARKRANEMEKKLLEKK"
BASE COUNT          795 a         1148 c         1048 g          760 t
ORIGIN      
        1 cccgcaggcg ttcgcgttca cgatcagacg agatcttgcg cgagacgcca atgtggtccc
       61 aggtcggcat gaataccagg tagcgaccgg gaagcgacag atacatcgtc gagcgcggac
      121 ccttgccggg cgacggctct ttgagaatct gcaccaggac gtcctggccc actttgatca
      181 ggtctttgat cggagtagga aagatgtctt tcagatcgcc gtcatccccc ccatcaagcg
      241 gttcaatgcc gctgagggtc agataggcat ctttttcgag accgatgtcg acgaatgccg
      301 attgggttcc gggcaccaca ttggctaccc gccccttgta aatgcacccg acgatctttt
      361 ccgtctcgcg ggtttcaaaa aacagttccg ttacctggtc attgtccagg atggctgcgc
      421 gggtctcata cgggcccacc gtcaccagaa tttttcgcga actcactcgt tacctcttgt
      481 tgtgtctgag aatgattggg ggacacctgc cgaacggcga cagccgctga aatgcggacc
      541 cgttcgatca gggtgatctg tggtcgccgg tcccccagac cggcgagaaa cgcctgaatg
      601 atcttggccc cggaaggaat gcccttcgcg ggagcagcaa aaatcagatg gagcgcgaga
      661 ggtggaccgt cgaccaggcg tgcttcgctg acggcacccg cgagaatgac ctcccgctcc
      721 tgaccgggtt tcccagctcg aatccgggtt tccgggtcga gaaagaaccg ccgcgccgtc
      781 tcgcgcaact cagcggcgtc aggagcaaat tccagccgaa actccgtctc tgcttcggcg
      841 gtcaggggtg gcattcccgg ctcgaccgcc accgccgaca caagaccgaa cccgatcggc
      901 aactgttgtc gcaccagatt cgccgcttct tccaccggga ccgactcacg cagggtcaga
      961 tccatcacct cacagcggct ggcgaatcca agggcgaccg gcggaccaaa gctcgtcttg
     1021 gggcgcggat gacacccctg cgtcacctga tacggcaacc cgcttcgtcg caccgcccgt
     1081 aacagatgat tcatggcatc gagatgcgaa atgaaacgcg ccgactcgtg gcgtgagaac
     1141 gtcaatcgaa ctttcatatc gcccatgcta tgcctccacc agcggcttca cgggctctgc
     1201 cgactccaca actcccggga cactcgacga accgacacga gccagatccg ttgcccgccg
     1261 atactcgtcg agatagtagg cccgcggcgt acgaaagtcg atgaactccc acgggagcgg
     1321 ctgatcgaca gacatctcac gggtataggt cgtgggatcg acggctgccc gctcaaacgc
     1381 ctgcttccag gcatcttcgc ggaatcggtc gcaccagcca tcgaaggtcg cccccgcctt
     1441 ccaggcctcg aagagcactt ttccaacctg ggcatcgccg cgcgagagta ccccttcgag
     1501 caggcagaga aactcatcac gccacgaaat gcgggcacga cttttccgca acccctggca
     1561 cactcgctgg cgacgctcga agagttcctg cggtgtcgcc tgtcgacacc actgaaacgg
     1621 tgtgaacggt ttgggaacga aaccggaaag actgatggtc agatccttac gcaggcgttc
     1681 gcgacgacag acgtcttcaa tgcgaaacac cagttcggga atcgcatcga gatcagcctc
     1741 ggtttcaaac ggcaacccca tcatgaagta gagtttgatc gattgatagt tcgatccctt
     1801 cgtggctgcg atcacatgca ggatctcgtc ttcggtgatc ttcttgtgaa tgatatcgcg
     1861 caaacgctga ctgccggcct cgggcgcgaa ggtcagcccg ctcttcttca tctcatcaat
     1921 acgttcgagc agggtcagag tctgatcgtt catgcgcaga gaaggcaccg cgagcgtctg
     1981 gtcgggataa cgtttctcgc gtccgagtcg atcgacaagc tcacccaggc aggagtagtc
     2041 agacgtcgac agcgaaatca aacccagcgt ctcttctccc gtggactgaa gaatctgttg
     2101 cgcccagcca cacaggtcat tcaccgaacg ctcgcgtttg ggccggtaga agaacccggc
     2161 attacagaag cggcacccct gaatgcagcc acgaaagatc tcgagtggca tgcgatcgtg
     2221 caccgattca atgttcgaaa tcaggggttt cagcgggggc gccgattgtc cgaactcttt
     2281 gtagacgcga cgcttgactg gagcaggaaa gcgcggcacg aaatatcctt cgatcgcggc
     2341 cagtcgttcg agcacctctt cgcgccgcca gcctctggtc ttggcctgta acagcaggtc
     2401 cgcgatatcc agaatggcat cttcaccatc gcccaccagt atcgcgtcaa agaattcagc
     2461 cacgggcacc gggttcgatg ccgacgggcc gccgccgatc acgagcggac cgctgcatcg
     2521 atctttggcc agtaacggga gcccggcgag atcgatgagg ttcagcacat tggtgaacgt
     2581 catctcgtag ggcagcgtga tgcccagcag atcgaaatcc cgcaccggcc gtttcgactc
     2641 aacggcgaac aggggcactc ccttgcgccg caacaggcgt tcgaaatcga tccagggcga
     2701 aaaggtgcgt tcgacctgaa tgcgttcatc acgcgccagt cgatcataca gaatgcgcag
     2761 cccgagattc gacattccca gttcgtacaa gtcaggaaat gccagacata ccgtcagctg
     2821 agggtttgcc tttgcctgag gctgattcca ttcgccgccg acgtagcggg ccggcttctc
     2881 gatgtcgagg agctcccagt cagagatgcc cgggatcacc gtgagggcct tccaccgcga
     2941 acggcagatt ccggaaaagt gaaaacgaga tccatctcgg gcccggagaa gtcgatatca
     3001 gccagcatct gatcaagact cacgcccatc actccggcga tctcccgacg cagctgctcg
     3061 agcaggcgcc gactttcggc cggtggaaga ttgagtcggt cacgcatcag ctgcagtttc
     3121 atcacctcga ccggcgtcaa acctcgttct gcctcggtca gctggccatc ttcgtgatgg
     3181 gaatcgtgta ccattatttc ttttccagca gcttcttttc catctcgttc gcccgttttc
     3241 gggcgtaggc attgcgtgaa tcgatgccca gagccgcatt gtaggctcgc agggccttgg
     3301 cgtgttcgcc ccgatgcaca tacaggtcac caaggttgat gaatccatcg atcatgtcgg
     3361 gcgccaactc ggtcgcccgc tgatactcgt gaatcgcttc ggcaatgcga ttactgcgat
     3421 gataggcaac acccagctga taatgaatca tcgggttctt ttcgtcgatc ttggctgcag
     3481 actcaaactc gcgctgcgaa gattcgatca tgccggcgag caggtacgtt tcgccgaggg
     3541 ccatgtgcac cttcaggtcg ccgcggtcgc gttgttgaaa tgccttgtag tggcgaatgg
     3601 cttcctgatg attccccgca tcctgagcga tttttcccag ggcatagcgg gacttgagat
     3661 gatacatgtc gtgcttgatg cactcgtgaa acaacttgat cccgtcttcc cggcggccgt
     3721 tatccatcag gaagacgccc agattgtagt a
//
LOCUS       DAORHD010000349         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26956_length_3749_cov_3.947212, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000349 DAORHD010000000
VERSION     DAORHD010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26956_length_3749_cov_3.947212"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..578)
                     /locus_tag="PKO06_08535"
     CDS             complement(36..578)
                     /locus_tag="PKO06_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69729.1"
                     /translation="MRTMLTMMRRCPANQGVTLVELLLVVALLAVVAAVAAPQFFRSG
                     SEAIADAKLERFKRSYATIRAAIATQIYMATDTQLLVDAASDGPVSDSGSRIAKLVGS
                     GYLDLASTQIENAKGEALSMRIFDAGTSGIDTTLPENNQKQLRFQVRVSGSDYNIDSY
                     LRSGHTLEEAWADHVKDLTP"
     gene            complement(578..919)
                     /locus_tag="PKO06_08540"
     CDS             complement(578..919)
                     /locus_tag="PKO06_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002750886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNV69730.1"
                     /translation="MSPTNAEVLQHILDEMAFLKEHAEKVSREQFLKDPVLKRAFVRC
                     WEIIGEATKNLDDSLRRDHPEVDWRGMAGMRDRLIHGYFSVDYELVWDAVQRKIPGTM
                     ETINRLRESMK"
     gene            complement(900..1226)
                     /locus_tag="PKO06_08545"
     CDS             complement(900..1226)
                     /locus_tag="PKO06_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV69731.1"
                     /translation="MVDKPVAVQMRKWVFETLKLAQPEWRRFGVKRLGVFGSVVRDEA
                     NSDSDVDILVEFERDRKNYDNFLDLAEYLERLMCRKVDLLTPEGLSPYLGPHILKEVR
                     YVSDEC"
     gene            complement(1236..2012)
                     /locus_tag="PKO06_08550"
     CDS             complement(1236..2012)
                     /locus_tag="PKO06_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNV69732.1"
                     /translation="MSKRSDQLLLADILLSIRKTQKYVKGLTFQSFSQDDRTIDAVTR
                     NLEIIGEAAKRISDGFKAEHASIEWHKMIGLRNRIVHDYFGIDLNIVWDTVVSYIPAL
                     KKTLSVLSPRRSARRGVTLMELLLVVALLAIVAAVGAPQVYNNLVGGVEKRIVWAATT
                     DFEYARSMGLSDAAAYADVEITAGSSQYQVATLTKMLDGEATFSAAAAFRFGATGTPE
                     LTPGKAALFPTFPFTVNVQSPAGAKIGEVTIAANGFITWK"
     gene            complement(2005..2310)
                     /locus_tag="PKO06_08555"
     CDS             complement(2005..2310)
                     /locus_tag="PKO06_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV69733.1"
                     /translation="MALHKRVSRAWILDAIRLVKPSLREKYGVKSIGLFGSFAREEAT
                     PDSDIDILVEFERPIGIEFVDLADEMESLLQRRVDLVSRNGVKKPYLKLIDKDLVYV"
     gene            complement(2316..3539)
                     /locus_tag="PKO06_08560"
     CDS             complement(2316..3539)
                     /locus_tag="PKO06_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV69734.1"
                     /translation="MIFHYKARTADGAESIGTLDAPDERTALAELTGRNLLVTKISRT
                     GNEWGLQSDVRFLTWIPARLFNAFLLQLSVMIRAGVPLVEALASLETGETHRTFRRIL
                     GDVRKEVEQGRMFSEALSTHPEVFDTFFINMVRIGETGGVLDKVLVKLASMQQRTIAL
                     RNQILGALAYPTLLVLVASVVLFVLFAFALPKFAEVFKQANFPMPTPTRIVLAIGDFC
                     RDYFRPLIALGIFAALASFLSALTARGRAFAGEVALKLPVVREVVRSYLIVHISESLG
                     MLLNAGVPLLELLGSVEQTLTMPTARGTLESMRNYVERGSTMRMALEGSVVFPSMALK
                     LIETGEKTGTLDKMFNEIAGYYDDNLQTALKAALSLLEPLLIFCMACVVGFIMLAVIL
                     PIFQMSQAFRGTIGG"
BASE COUNT          849 a         1054 c         1039 g          807 t
ORIGIN      
        1 cattgagagc cacagaactt tattgatcat cgttttcatg gcgtaagatc tttcacatgg
       61 tctgcccagg cttcctcgag ggtatgcccg gatcgcagat aggaatcgat attgtagtct
      121 gaaccggaaa cgcgcacctg aaagcgcagc tgcttctggt tgttctcggg taacgtcgta
      181 tcgataccgc tggttccggc gtcgaagatg cgcatcgaca gggcctcgcc cttggcgttc
      241 tcgatctggg tcgaggcaag gtcgagatac cccgaaccga cgagctttgc aatgcgcgaa
      301 ccagagtcac tcaccggccc gtcagatgct gcgtcgacga gcagttgggt gtcggtggcc
      361 atgtagatct gcgtggcaat ggccgcgcga atcgtggcgt agctgcgctt gaaccgctca
      421 agtttcgcat cggcgatggc ttcgctgccc gagcgaaaga actgcggtgc cgcgaccgcc
      481 gccaccaccg ccagcagcgc cacaaccagc aacaattcga cgagcgtgac cccctggttt
      541 gcaggacacc gtcgcatcat ggtcagcatc gttcgcatca cttcatgctc tctctcaaac
      601 gatttattgt ctccatggtt ccgggaatct ttctctgcac cgcgtcccaa acaagctcat
      661 agtcgacgct gaagtatccg tgaataagtc tatctctcat tccggccata ccacgccagt
      721 ccacttcagg gtggtcgcgc ctcaaggaat catccagatt tttcgtagct tcgccgataa
      781 tctcccagca tcgaacaaac gcacgtttca gaacgggatc tttcagaaac tgctcgcggg
      841 aaactttctc ggcatgttct ttcaaaaagg ccatttcatc caagatgtgc tgcaatacct
      901 cagcattcgt cggagacata tcgtacttcc ttaagaatgt gtgggccaag atatgggctg
      961 agtccttccg gggtcagaag atccactttt cggcacatca ggcgttcgag atactcggcc
     1021 aggtcaagaa aattgtcata gttttttcga tcccgctcga actccaccag aatgtctaca
     1081 tcactatcag aattcgcttc atctcgcaca actgagccga aaacaccgag gcgtttcacg
     1141 ccaaatcgac gccattcggg ctgcgccaat ttcaaagttt caaacaccca ttttcgcatt
     1201 tgaactgcca ccggtttatc taccatacag gtcgctcact tccaggtgat gaagccgttg
     1261 gcggcgatgg tgacctcacc tatctttgcg cccgcgggag actggacatt caccgtaaac
     1321 gggaaagtcg gaaacaatgc agccttgccg ggagtcagct cgggcgtacc ggtcgctccg
     1381 aaccggaacg cggcggcagc gctgaatgtc gcctcaccgt cgagcatttt cgtgagggtt
     1441 gcgacctgat actggctgct gccggcggtg atctcgacat cagcatacgc ggcagcgtct
     1501 gacaagccca tggagcgagc gtattcgaag tcggtcgtcg cggcccagac gatgcgcttc
     1561 tcgaccccgc cgacgaggtt gttgtaaacc tgcggcgccc cgaccgcggc gacgatcgcc
     1621 agcagcgcta cgacgagcag cagctccatg agcgtgacgc cacgtctcgc cgaccgacgg
     1681 ggagaaagca cagacaacgt ttttttcagc gcaggaatgt atgagacaac cgtatcccag
     1741 acgatattga ggtcgatacc gaagtagtca tgtacaatac gatttctcag accaatcatc
     1801 ttgtgccact caatgctcgc atgttctgct ttgaaaccgt cagagatgcg tttcgccgct
     1861 tccccgatga tttccaggtt tctcgttact gcatcaattg ttcggtcgtc ctgcgaaaag
     1921 gactgaaacg taagtccttt cacatacttc tgtgttttgc gaatcgaaag cagaatatcg
     1981 gccagcagta gttgatcgga tcgtttagac atagacaaga tctttatcga tcaatttcag
     2041 gtatggtttt ttcacaccat ttcgagaaac caggtcgacc cttcgctgca gaagcgattc
     2101 catctcatct gcaagatcaa cgaactcaat gccgatgggt ctttcaaatt caaccaagat
     2161 gtcgatatcg ctatccggcg tcgcttcttc tctggcgaac gaaccgaaga gcccgattga
     2221 tttcactccg tatttttctc gcagagacgg cttcacgagc cgaatcgcgt ccagaatcca
     2281 cgcacgtgaa acccgtttat gcaatgccat ttcctttagc cgccgatggt gccgcggaag
     2341 gcctgggaca tctggaagat gggcaggatg acggcaagca tgatgaagcc cacgacgcag
     2401 gccatgcaga aaatcaacag gggttcgagc aggctgagcg cggctttgag cgcggtctgc
     2461 aggttgtcgt cgtagtagcc ggcgatctcg ttgaacatct tatcgagcgt tccggttttc
     2521 tcaccggttt caataagttt cagagccatg gagggaaaga cgacgctgcc ctcgagggcc
     2581 atgcgcatgg tgctgccgcg ctcgacgtag ttgcgcatcg attcgagcgt gccgcgcgcc
     2641 gttggcatgg taagagtctg ttcgaccgag ccgagcagtt cgagcagggg caccccggcg
     2701 ttgagcagca ttcccagtga ttcagagata tggacgatca gataactgcg cacgacctcg
     2761 cgcaccacgg gaagcttgag agcaacttct ccggcgaagg cgcgaccgcg ggcggtgagc
     2821 gctgagagga acgatgcgag ggccgcgaag atgccaagcg cgatcagcgg acggaagtag
     2881 tcgcggcaga aatcgccgat cgccagcacg atacgggtcg gggtcggcat cggaaagttc
     2941 gcctgtttga agacttcggc gaacttcggc aacgcgaagg caaacagcac gaagaggacg
     3001 acgctggcca cgagtaccaa cagtgtcgga tacgccagag cgccgagaat ctggttgcgc
     3061 agcgcgatgg tgcgctgctg catgctcgcg agcttcacga gcaccttgtc gagcacgccg
     3121 ccggtctcac cgatgcgcac catgttgatg aagaaggtat cgaagacctc ggggtgcgtc
     3181 gaaagcgctt cggaaaacat gcgtccctgc tcgacttcct tgcggacatc gccgaggatt
     3241 cggcgaaacg tacgatgcgt ttcgcccgtc tcaagcgaag cgagcgcttc aaccaatggg
     3301 actccggcac gaatcatgac cgacagctgc agcagaaacg cgttgaacag tcgggcgggg
     3361 atccaggtca ggaagcgaac gtcggactgg agtccccact cgttaccggt gcgtgagatc
     3421 ttggtgacca gcaggttgcg tccggtcagt tcggcaagcg ccgtgcgttc atcaggggca
     3481 tcgagcgtgc caatggattc agctccatca gcggtgcggg ctttatagtg gaagatcaca
     3541 ggtcgacatc ctcttgcagg tagcggattt cattcacaga cagcgggaca gttccctgaa
     3601 acagatacgt catggtaaca tttcgacaac ggtgcgacaa catcgccgaa ccgcaaattt
     3661 tcgtcgagct gaaaggcatg gggtatgaac acggtgaatc tgctggatca gctgtctccc
     3721 aagcactgtc gctggctcgg actgctcat
//
LOCUS       DAORHD010000350         3747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_26978_length_3747_cov_3.223185, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000350 DAORHD010000000
VERSION     DAORHD010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26978_length_3747_cov_3.223185"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..883
                     /locus_tag="PKO06_08565"
     CDS             305..883
                     /locus_tag="PKO06_08565"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HNV69735.1"
                     /translation="MMKAAEARIFWSTYMILDKRNREEMPLKTQISIAFDILNYNQGL
                     IQFADGKANGLLLINSIFIASVAPFIETFRKGAGGFGMLLLGGFFVLCVVSIMLSMSV
                     IMNRKLPEVEDKNRGIIFYGHIVETRSADGYIHEFHATEAARFKDNLLTNVYTVSQIA
                     TRKYQIYSYAQALTLLSCITWIITVAYLLFSH"
     gene            complement(908..3202)
                     /locus_tag="PKO06_08570"
     CDS             complement(908..3202)
                     /locus_tag="PKO06_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HNV69736.1"
                     /translation="MTTEFLTVQIERVTYTNAQNGYSVLKATSKGFRELITLVGVFAN
                     IHPGEELRVTGTWACHPQFGQQFHVQHYSTLKPATLKGIEKYLGSGLIKGVGPVTAKR
                     LVAQFHEQTLEIIEHQPELLTSCPGIGSFRAEKIISGWQQQKAIQDVMVFLQGHGIST
                     AYAVRIFKHFGGEAVKKVADNPYLLAYEIRGIGFKTADKIAAEFGISGADPRRLEAGV
                     LYVLSQHLDEGHLFLPLKLLVDKSRDMLGIEQVSDIETAIESQIAAGRATSRTFRDQR
                     LIYLPFAYRHEQGCVDQLSRRLDTSPSLARERLLALLAQVEARSNLSLSEQQKLAVEK
                     SLRTRLLVITGGPGTGKTTTLNSLVQAHRLAGREVALASPTGRAAKRLSEVTGLEAKT
                     IHRLLEFDPSRGAFKHDAKNPLQCQTLVIDEASMLDQELFHGVLKALPPQTSLLLVGD
                     VDQLPSVGAGLVLKSLIDSGVIPVVRLDTIFRQAQGSLIIANAHKINTGRFPHLQEPD
                     GKTQTDFYFLEHDDKEKLIAQLKLVLTSSLPKKFGYHPIHDIQVLTPLNRGPLGATLL
                     NQVLQEALNPAAPDKPELTHMNRRFRAGDKVLQTRNNYDLEVFNGDVGIIRQVEPEDQ
                     ELVIEFPQGERVFQNADLLDLTHAFAMSVHKSQGSEYPGVVLLLTNSHYVMLQRNLLY
                     TGVTRARKTLVIMGTKQAIALAVKNNRTIHRYTIFSELLQEAFATRRTSDNPSAKPGM
                     ATGPGGFRESSSDFAEANDQTVVE"
     gene            3319..>3747
                     /locus_tag="PKO06_08575"
     CDS             3319..>3747
                     /locus_tag="PKO06_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69737.1"
                     /translation="MKHLFSRGLFLSLARIVGWGWVIFWFLCVTAYPPFASSGLRFRI
                     SAPPCLTPGARQNIVLYLYTDDGIGARAGQAALALRRPDGRWLGPWTMLPSKPGVCVA
                     EFQIPAEASEGTVEPWLSLDDRPFMPLPGAALQVRHLAQLA"
BASE COUNT          793 a         1001 c         1083 g          870 t
ORIGIN      
        1 aaggccgcga agtcgactgc catgaggaac gagagaagaa tgcagatgct cacattgata
       61 ctacgccgcc gaatcatacc gtaccccctt gaagacgtag gctaaatcag gatttctccc
      121 taatttcttc caattttaac aaacgaatgg gggaccgtca aggaaccagc cgtacatctt
      181 ccgttcggca cgcggaattt cgtcgcggcg cgaaactggc tgatcgtgga taccttgatc
      241 tgatgcggca tgtagaggga atggtgtcga cagaaagtcc cgtgaaaaaa agtgagtttg
      301 tggtatgatg aaggccgctg aagcacgcat tttctggagt acatacatga ttctcgacaa
      361 acgaaaccgg gaagaaatgc cgctgaaaac tcagatatcg attgcgttcg atattttgaa
      421 ttacaaccag gggctgattc agttcgccga cgggaaagcc aacggccttc tgctgattaa
      481 ttcgatcttc atcgcctcgg tggcgccatt catcgagacc ttccgcaaag gtgccggggg
      541 attcggcatg cttctgcttg ggggcttctt tgtcttgtgc gtggtctcga ttatgctctc
      601 gatgagcgtg atcatgaacc gcaagctgcc cgaggttgaa gacaagaacc gcggcattat
      661 tttttacggt cacattgtcg aaacccgctc agctgacggg tacattcacg agtttcacgc
      721 cacggaagcc gccaggttta aagataatct gcttaccaat gtgtacaccg tctctcagat
      781 tgccaccagg aaatatcaga tctacagtta tgctcaggca ctgacacttc tttcctgtat
      841 cacctggatc atcacggtag cgtatctcct cttctctcac tgaagcgagc gcccgcgtaa
      901 ccgttcctca ttcgacgacg gtctgatcat tcgcctcggc aaaatcagag ctggattcgc
      961 ggaatccacc gggaccggtt gccatacccg gttttgccga cgggttgtcg gaggttctgc
     1021 gtgtggcgaa ggcttcctgt agcagttcac tgaagatcgt ataccggtga atggttcgat
     1081 tgttttttac cgcaagagca atcgcctgct tggtgcccat gatgaccagg gttttgcggg
     1141 ctcgggtcac gccggtgtac agaagatttc gctgtaacat gacatagtga gaattggtca
     1201 gcagcagcac gacacccgga tattcgcttc cctgtgattt atgaacggac atggcgaagg
     1261 catgcgtcag atcgagaaga tcggcgttct ggaatacccg ttcaccctga ggaaactcga
     1321 tcacgagttc ctgatcttcg ggctcaacct gccgaataat accgacatcg ccattgaaca
     1381 cctcgagatc atagttgttg cgggtctgca aaaccttgtc tccggctcgg aatcggcggt
     1441 tcatatgcgt cagttcgggt ttgtcaggtg cagcgggatt cagtgcctcc tggagaacct
     1501 gattcagaag cgtcgctccg agtggaccgc gattgagagg tgtcaggacc tgtatgtcgt
     1561 gaatcgggtg atacccgaac ttcttcggaa gactcgacgt gagaaccagc ttcaactgcg
     1621 cgatcaattt ttccttgtcg tcatgttcga gaaagtagaa atcggtttgc gttttgccgt
     1681 cgggttcttg caggtgtggg aaccggccgg tattgatctt gtgggcattg gcgatgatca
     1741 gtgatccctg agcctgacga aagatcgtgt cgagacgcac gaccgggatg acaccgctgt
     1801 cgatcaggct cttcaacact aacccggctc cgaccgaggg gagctgatcg acgtcaccga
     1861 ccaggagcaa agatgtctga ggcggcaacg ccttgagcac tccgtgaaag agctcctgat
     1921 caagcatcga agcttcatcg atgaccagcg tctggcattg cagcgggttt tttgcatcgt
     1981 gcttgaatgc ccccctcgaa gggtcgaact cgagcaggcg gtgaatggtc ttcgcttcga
     2041 ggccggtcac ttcagaaagc cgcttggctg cccttcctgt cgggctggcc agagcgactt
     2101 cacgacccgc aagtcgatgg gcttgtacca gtgagttgag cgtggttgtc ttgccggtgc
     2161 ccgggccgcc ggtgatcacc agaagccgtg tgcgcagcga cttttcgacc gccagcttct
     2221 gttgttctga cagcgagagg tttgaccggg cttcgacctg agcgagaagt gcgaggagtc
     2281 tttcacgggc gagactcggc gaagtgtcga ggcggcggct gagctgatcg acacagccct
     2341 gctcatggcg ataggcaaag ggcagataga tcaagcgctg atcacgaaac gtgcgcgagg
     2401 tggcacggcc agcggcgatc tgggattcga tcgctgtttc gatgtcagaa acctgctcga
     2461 ttccgagcat gtcgcgactc ttgtcgacga gcagtttcag gggaagaaac agatgccctt
     2521 catcgaggtg ctgcgaaagt acgtagagca cgccggcttc gagacgacga ggatcagctc
     2581 ccgaaatgcc gaattccgcc gcgatcttgt cggcggtctt gaagccgata ccgcgaattt
     2641 cgtaggccag cagatacgga ttgtcagcga ctttcttgac cgcctcgccc ccgaaatgct
     2701 tgaaaatgcg cacggcgtag gctgttgaaa tgccgtgccc ctgcagaaag accatgacat
     2761 cctgaatggc tttctgctgc tgccagcccg aaatgatctt ctcggcccga aaagaaccga
     2821 tgccggggca cgacgtgagc agttctggtt ggtgctcaat aatctcgagc gtctgttcgt
     2881 gaaattgcgc gaccaggcgc tttgccgtga ctggcccgac ccctttgatc aaccctgagc
     2941 caagatactt ttcgattccc ttgagggttg ccggtttcag ggtggaataa tgctgaacgt
     3001 gaaactgctg gccgaactgc gggtgacagg cccaggttcc ggtgacgcgc agttcttcgc
     3061 ccgggtgaat gttcgcgaag acgccgacga gggtaatcag ctcacgaaag cctttgctcg
     3121 tggctttcaa caccgaatac ccgttctggg cattggtgta ggtcacgcgt tcgatctgaa
     3181 ccgtcagaaa ttcggttgtc acggagggtg attataacgg aaaagcccac gggtgcaaag
     3241 tcccaaatcc tgtcatacag ccggttttgt tgacagatgg aggtctccga tggtaccttt
     3301 tgagccggta cacgtgacgt gaagcacctt ttctctcgcg gcctcttcct ttccctggcc
     3361 cgtattgtgg gctggggttg ggtgattttc tggttcctgt gcgtgaccgc gtatccaccg
     3421 ttcgcctcgt cggggttgag attccgcatt tccgcgcccc cgtgtctcac cccgggagct
     3481 cgtcagaaca tcgtgctcta tctgtacacg gacgacggca tcggcgcgcg ggccggacag
     3541 gctgctctgg ctctgcggcg acctgacgga cgctggcttg gaccctggac catgctgccg
     3601 agcaagcccg gtgtctgcgt ggcggaattc cagattcctg ccgaagcgag cgaaggaacg
     3661 gtagagccct ggctctctct cgatgaccga ccgttcatgc cactgccagg agcggctctg
     3721 caggtgcggc acctggcaca attggca
//
LOCUS       DAORHD010000351         3744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27010_length_3744_cov_4.140689, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000351 DAORHD010000000
VERSION     DAORHD010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27010_length_3744_cov_4.140689"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKO06_08580"
     CDS             complement(<1..434)
                     /locus_tag="PKO06_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69738.1"
                     /translation="MLYPLWMCRRVTVLWLCVLCLIASGPACAQVDRLNALITSGQTD
                     KPALDQLLTSLSEEQREQCVAALVTHLESSDWRHREAAAYVAGQLGPKAGPIARQLIK
                     AMACDMSNVAWEVAPALGKIGAAAVPAVIDELKACQALPDKVG"
     gene            complement(510..2996)
                     /locus_tag="PKO06_08585"
     CDS             complement(510..2996)
                     /locus_tag="PKO06_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69739.1"
                     /translation="MPEGSNRERDLVLAPNEFAFISDQTKGNINVYVGSYKTSLANTD
                     QPVTFNYQSKRFERCTLEQAIQLFAIAPEGWYLVMKNPAKDNVHPKNSTVNNLCELEI
                     GKKVNAPGPISFALWPGQMVKIIQGHHLRSNQYLVVRVYDEEAAKKNWQKAVMKPQNP
                     GSPEEAGKDIAAEDLTMGKQLVIKGTEVSFYIPPTGIEVVPSDDGIFVREAVTLERLE
                     YCILLDENGNKRYIKGPDVVFPNPTETFVEKHGSRKFKAIELNEISGLYIKVIAPYSE
                     GGKEYKVGDELFLTGKDTMIYFPRPEHAIIKYGDQEIHYAVAIPAGEGRYVLNRLTGK
                     IQLHKGPAMLLPDPRNEVVVRRVLDPKVCELWFPGNTEALEYNRSLKQMSTGKAQEPY
                     VADGEVRRLHAAAPAPLAKKAESAAEGFAGDDFHRRANFTQPRTITLDTKYEGAVSIS
                     VWTGYAVLVVNKAGERKVVAGPQTYLLEYDESLEALELSTGTPKSSDKTLKTSYLRIL
                     HNKISDVVEAETADLVRVRILVSYRVNFEGEEGRWFNVENYVKFLTDHMRSLISNRIK
                     EMTIESFYARAINLVRDMVLGVTGPDGKRSGRIFKENGMVIYDVEVLDVKIGDTGIEK
                     LLVEAQHSVVQQNLEVSAQKRQLEYVRQTETVKQQVAQAKATTKTLEIDIQTEEVKRQ
                     LMLNLAKIESEVEGRKLALEAKFNDQELLTKVETAELARRKAAADLELAVAHRRLEQQ
                     LESLRAEVKAVVDKAEAVSPELIAALQAFSDRALAERMAESMAPLAILGGKSIAEVFA
                     TLLQGTPIEAVLKRKAVSAPVSTPTPPATK"
     gene            3113..>3744
                     /locus_tag="PKO06_08590"
     CDS             3113..>3744
                     /locus_tag="PKO06_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNV69740.1"
                     /translation="MSTSIHEWPDSLDKAREIQEQLRSRVCLTDDSRPIRFVAGIDCS
                     AAFWSHDLWATIIVIDRNTGQLADRATAFGRAPFPYVPGFLTFREGPLVVEAFSRLTV
                     VPDLLVFDGQGIAHPRGLGLAAHLGVWFDLPSIGCAKTHFFGDYAEPDPEPGDFSPLL
                     DGERLIGAVLRTRRRARPVFISPGHRVSVDGARNLVWSFCAGYRLPEPTR"
BASE COUNT          716 a         1097 c         1058 g          873 t
ORIGIN      
        1 ccgaccttgt ccggaagcgc ctgacaggcc ttcaattcgt cgatcactgc gggaaccgcg
       61 gccgcgccga tcttccccag tgccggggcg acctcccagg ccacgttcga catgtcgcac
      121 gccatggcct tgatcagctg ccgggcgatt ggcccggctt tgggtcctag ctggccggcc
      181 acgtaagcgg cagcttcacg gtgtctccag tcactcgatt ccagatgagt aaccagagcc
      241 gccacacatt gttcccgctg ttcttcagaa agtgaggtca gaagctgatc cagggcaggc
      301 ttgtcggtct ggcccgaggt gatcagtgcg ttcaggcgat ccacctgcgc acaggcaggc
      361 cctgacgcga tgaggcagag aacacacagc cacagaacgg taacacggcg gcacatccac
      421 aacgggtata acatcagcaa cctcctgggc acaaaaactc ccccggaatt tgtcctaccc
      481 gggggagctc gaatgtctgt ctctctgaac tatttcgtgg caggcggagt gggtgtcgac
      541 accggggccg acacggcttt gcgcttcagc acggcctcga tcggggttcc ctgcaagagc
      601 gtcgcgaaca cttcggcgat actctttccg cccagaatgg ccagcggtgc cattgactcg
      661 gccatgcgtt cggccagagc ccggtcgctg aaggcctgca aagccgcgat gagttcgggc
      721 gacacggcct cagccttgtc gaccacggcc ttcacttcgg cacgcagcga ttcgagctgc
      781 tgctcgagtc gtcggtgcgc tacggccagt tcgagatcgg ctgccgcttt gcgccgggcg
      841 agttccgcgg tctcgacctt ggtgagcagt tcctgatcgt tgaacttcgc ttccagggcc
      901 aatttccggc cttcgacctc ggactcgatc ttggccagat tcaacatcaa ctggcgtttg
      961 acttcttcgg tctgaatgtc gatctcgagg gtcttggtcg tcgccttcgc ctgagcgacc
     1021 tgctgtttca ccgtctcggt ctggcgcacg tattcgagtt gccgcttctg cgccgacact
     1081 tcgagattct gctgcacgac ggaatgctgg gcttcgacga gcagtttttc gataccggtg
     1141 tcgccgatct tcacgtcgag cacttcgacg tcgtagatca ccatgccgtt ttccttgaag
     1201 atgcggccag agcgtttgcc atcgggcccc gtcactccaa ggaccatgtc gcgtacgaga
     1261 ttgatcgccc gcgcgtagaa gctctcgatc gtcatttcct tgatgcggtt actgatcaac
     1321 gagcgcatat ggtcggtgag gaacttgaca tagttctcga cgttaaacca gcgtccttcc
     1381 tcaccttcga agttgacgcg gtaggacacg agaatgcgca ccctcaccag gtcagcggtc
     1441 tcggcttcga cgacatcgga aatcttgtta tgcagaatgc gcagatacga ggtcttgagc
     1501 gttttgtccg agctcttggg cgtgccggtc gagagttcaa gggcttcaag actctcatcg
     1561 tattcgagca gataggtctg cgggccggcg accaccttgc gttcaccggc cttgttcacc
     1621 acgagaaccg cgtagccggt ccagacggag atggaaaccg cgccttcata cttggtatcg
     1681 agcgtgatgg tgcggggctg cgtgaagttc gcgcgacgat gaaaatcatc accggcaaac
     1741 ccttccgcgg cactttcggc tttcttcgcg agcggcgcgg gggcagccgc atgcaggcgg
     1801 cgcacttcgc catcggccac gtagggttcc tgcgctttgc cggtcgacat ctgtttcagc
     1861 gagcggttgt attcgagcgc ttcggtattc cccgggaacc acagttcaca caccttggga
     1921 tcgagaacgc gccgtacgac cacttcgttg cgcggatcag gcaacagcat cgccggccct
     1981 ttgtgcaact ggatcttgcc ggtcaggcga ttgagaacgt accggccctc accggcggga
     2041 atcgcgacgg cataatggat ttcctggtca ccgtatttga tgatcgcgtg ttccgggcgg
     2101 gggaaataga tcatcgtgtc tttgccggtg agaaacagtt catcaccgac cttgtactct
     2161 ttgccaccct cagagtatgg cgcaatcacc ttgatgtaca agccgctgat ctcgttcagt
     2221 tcgatggcct tgaattttcg tgacccatgt ttttcaacaa aggtttcggt cgggttggga
     2281 aacacgacat caggcccttt gatgtaacgc ttgttgccat tttcatcgag cagaatgcag
     2341 tattcaagcc tttcaagtgt aacagcttct cggacaaaga tgccgtcgtc agagggaacg
     2401 acttcgatgc cggtcggagg aatatagaac gagacttcgg ttcccttgat gaccagctgt
     2461 ttgcccatgg tgagatcttc ggcggcaata tccttgccgg cctcttcggg gctgcccggg
     2521 ttctgcggtt tcatgacggc cttctgccag tttttcttgg cggcctcttc gtcgtagaca
     2581 cgcacgacca gatactgatt cgaacgcaga tgatgaccct gaatgatctt gaccatttgt
     2641 ccgggccaca aggcgaacga aatcgggcct ggagcattaa cctttttgcc gatttccagt
     2701 tcgcagaggt tgttcaccgt gctgttcttt ggatgcacat tgtcttttgc cgggttcttc
     2761 atcaccagat accaaccctc gggcgcaatg gcaaagagtt gaatagcctg ttccagagtg
     2821 cagcgctcga accgcttact ctgatagttg aaggtgaccg gttggtccgt attggcaaga
     2881 ctggtcttgt acgaaccgac atacacattg atgttgcctt tggtctgatc agagatgaag
     2941 gcaaactcat taggagccag taccaggtcg cgctcgcgat tcgatccttc gggcatgtca
     3001 tcgtctcctg aacgaagatt tctggtcttt atcaccgaga cctctcctca cgttgagtac
     3061 tgtggtcatg gtacagtaac atgaaacgtc agaaggttgc agaaaattga ccatgtccac
     3121 ttccattcac gaatggcctg acagccttga caaagcccga gagattcagg agcaactgcg
     3181 ctcacgggtc tgtctgaccg atgactcacg accgattcgg ttcgttgccg gtatcgattg
     3241 ttcggcggca ttctggagcc acgatctctg ggcgaccatc atcgtcatcg atcgcaacac
     3301 cgggcagctg gcagatcgtg cgaccgcctt cgggcgagcc ccgtttccgt atgttcccgg
     3361 ttttctgacc tttcgggaag ggccgctggt cgtggaggcc ttttcacgac tcaccgtcgt
     3421 gccagacctg ctcgtgttcg acggccaggg aatcgcacac ccacgcggcc tcggtctggc
     3481 agcacatttg ggggtctggt tcgatctgcc gagcatcggg tgcgccaaga cccacttttt
     3541 tggcgactat gccgaaccag accccgaacc cggggatttt tcgccactgc tcgacggaga
     3601 acgcctgatc ggcgcggtgc tgcgtacacg acgtcgagcc aggccagtat tcatttctcc
     3661 cggtcatcga gtcagtgtcg acggagccag aaaccttgtc tggagcttct gcgccggcta
     3721 tcgacttccg gaacccacac gatt
//
LOCUS       DAORHD010000352         3733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27135_length_3733_cov_3.844209, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000352 DAORHD010000000
VERSION     DAORHD010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27135_length_3733_cov_3.844209"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..2075
                     /locus_tag="PKO06_08595"
     CDS             138..2075
                     /locus_tag="PKO06_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV69741.1"
                     /translation="MKQPLIMGLLVVVPLALLAWLGARLERAERENVARTYDELLRSR
                     LLEVDRTLSGLLEERGRAIEQALIDDLPDATRRAELYRSLPWLTQIFAQNQDGRVEVP
                     ALEDGVSSEEHGFLERCRPLFDQKLLLTPPADAMATPAMKVVATAVANVPAPSVVTRD
                     TRNSAPADQASPTNESGSSTRMEVASLPLLVPQQRGPLPLTQQELDQAMQQDVSQVIP
                     MEQQQVPANIERLVDRAFQRLGGPASRRGISGSAGWYPYYWGHDVRLLYLVKTTSGGV
                     RGAELSRSRLIADLIGRIPEPAPDDAILREGSMLLLDGGARPLYRWGRYLPAAGAKPR
                     LQVALSAPLQQWSIQYFVAPGFVEATAATSRSLPLLLSLAGVAVALSALAVVLYRDQQ
                     RELLEAAQRVTFVNQVSHELKTPLTNIRLHAELLEQELPEDDEDGRRRIGVVISESQR
                     LGRLIGNVLTFSRQQRGRMALRRVSAVPDDTVRQVLERFAPAFNSLNLAVTANLRAGE
                     PVQIDPDALEQILNNLLSNVEKYAARGGAMLVSTRVGADRFTLVVCDRGQGIPSTQSD
                     YIFQPFARLHDDLTEGVSGAGIGLSISRELARRHGGDLRLAQNAEERREWCAETPFSD
                     TGGACFVATLAVSDAAEEKGS"
     gene            2072..2809
                     /locus_tag="PKO06_08600"
     CDS             2072..2809
                     /locus_tag="PKO06_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020681197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV69742.1"
                     /translation="MKVLIVEDDRLMRQALRDILYSDGYQVLEAADGEAGLEAFSRHR
                     PDFVCLDVMMPKCDGYEVCRRLRRQDAQVPIVFISAKGEEIDKVLGLELGADDFIVKP
                     FGVREVTARIRAVTRRCLARNQGGGPLNVDVNEWPESFVLGDVTIFPRELRARRGDQT
                     FEVSLREVRLLKVFHDRPGEVLDRTTLFRAGWGEDHIASSRTLDQHIAQLRKRLEIDP
                     RNPRIITTVHGAGYRYDLVPTDPDKRR"
     gene            complement(2941..3657)
                     /locus_tag="PKO06_08605"
     CDS             complement(2941..3657)
                     /locus_tag="PKO06_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69743.1"
                     /translation="MSRGKKILFGLVAVLVVLVAVPYYTSYQQRAMFSSIGATLSRPD
                     PAMEAIDRLLSSLTLGNIAFNTPTTIPLGEAQVIHLALSTRETVDNLKTLIETEGQKT
                     GAQIRVGSEMEAHLTGKGFKIQAITPETQAVSAVETTEWKWEVEPTEGGPQRLHLSMS
                     AVLNVDGQRVPRAIRTFERTIEIQVTWTRQISDFVGNNWQWLWATILVPVIGYLYQIR
                     SHSGLPRPSPADASKLDDHS"
BASE COUNT          708 a         1021 c         1218 g          786 t
ORIGIN      
        1 tgaacaagtt cgccacgcaa aacgaaacgg atgccaagaa tcttgatgac gaaagctgga
       61 ccaagactcg caagggaatg cgaaaggatc agcatgccat cgatatgcag cagacgtggt
      121 aaggagcttt tgtgcgattg aagcagccgc tcatcatggg attgctcgtg gtcgtgcccc
      181 tggccctgtt ggcctggctg ggcgcgcgac tcgagcgggc cgagcgtgag aacgtggcac
      241 gcacctatga tgagctgctg cgcagtcgtc tgctggaggt ggatcgaacc ctgtcaggcc
      301 tgctcgaaga gcgagggaga gcgatcgagc aggcgctgat cgatgatctg cctgatgcga
      361 ctcgacgggc cgaactgtac cggtcactgc cctggttgac gcagatattt gcccagaatc
      421 aggatggtcg ggtcgaagtg ccggccctgg aagatggggt gtcgagtgaa gagcacgggt
      481 ttctggaacg gtgccgcccg ttgttcgacc agaagttgtt gctgactccg ccggcagacg
      541 cgatggcaac accagcgatg aaagtggttg cgaccgcggt ggcgaatgtg ccagcccctt
      601 ccgtggtcac tcgcgacacg aggaacagcg ctcctgcgga tcaagccagt ccgaccaatg
      661 agagcggttc ctcgactcgg atggaagtcg cttcgctgcc cttgctggtg ccgcaacagc
      721 ggggccccct gcccctgacc cagcaggagc tggatcaggc gatgcagcag gatgtcagcc
      781 aggtcattcc catggagcag caacaggtgc cggcgaacat tgaacggctg gttgatcggg
      841 cttttcaacg acttggcggt ccggcttccc gtcggggcat ctcaggaagc gccggctggt
      901 acccgtatta ctggggtcac gacgtgcgac tcttgtatct ggtgaagacc acgtctggag
      961 gagtccgagg agctgaactg tctcgctcac gtctgatcgc cgatctgatt gggcgtattc
     1021 cggaaccggc acccgatgat gcgatactgc gcgaggggtc gatgctgttg ctcgacggag
     1081 gggctcgccc gctctatcgc tggggacgct atctgccggc cgccggggcg aaaccgcgat
     1141 tgcaggtggc gctgagcgcg cctctgcagc agtggtcgat tcagtatttc gtggccccgg
     1201 gttttgtcga agccacggcg gcgacttccc gctctctgcc gctgctgctg tctctcgccg
     1261 gtgtagccgt cgcgctctcg gccctggctg tcgttctgta tcgagatcag cagcgggaac
     1321 tgctcgaagc cgcgcagcgc gtgacttttg tgaatcaggt ctcgcacgaa ctgaaaactc
     1381 cgctgaccaa cattcgactg catgcagaac tgctcgaaca ggagctgcct gaagacgatg
     1441 aagacgggcg gcggagaatc ggggtcgtga tctccgagag tcagcgactg ggccggttga
     1501 ttggaaacgt gttgacgttt tcgcgacagc agcgtgggcg catggcgtta cggcgggtct
     1561 cggccgttcc ggatgacacg gtacgacagg tgctcgaacg atttgccccg gcattcaata
     1621 gtctgaatct ggcggttacg gctaatcttc gagccggtga accagtacag atcgatcctg
     1681 atgcgctcga gcagattctc aacaacctgt tgagtaacgt cgagaagtat gcggcgaggg
     1741 gaggggcgat gctggtgtcg acccgggtcg gtgccgaccg attcacgctt gtcgtctgtg
     1801 atcgggggca gggaattccg tcgactcagt cggactacat ctttcaaccg tttgcccgtc
     1861 tgcacgatga cctgacggaa ggggtgagcg gagcgggcat cgggttgtcg atctcgcgag
     1921 aactggcgcg tcgtcatggt ggtgacctgc gtttggctca gaatgcggaa gagagacgcg
     1981 agtggtgcgc tgagacgcct ttttcagata ctggtggcgc ttgtttcgtg gccacgctcg
     2041 cggtatcaga cgcggccgag gagaaaggct catgaaagta ctcattgtcg aagacgatcg
     2101 tctgatgcgt caggcgctgc gagatatctt gtactcagat ggatatcagg tgctggaggc
     2161 cgccgatggc gaagccggtc tcgaggcatt ctccaggcac cgacctgatt tcgtctgtct
     2221 cgatgtcatg atgccgaagt gcgacgggta tgaggtctgt cgtcgcctgc gacgtcagga
     2281 cgcccaggtg cccatcgtct tcatttccgc gaaaggcgaa gaaatcgaca aggtgctggg
     2341 tcttgaactg ggagcggatg acttcatcgt caagcctttc ggcgtgcgcg aagtgaccgc
     2401 tcgcattcgt gccgtcaccc gccgctgtct ggcgcgaaat cagggcggtg ggccgttgaa
     2461 cgtcgatgtg aacgaatggc ccgagtcgtt cgtcctgggc gatgtgacca tttttccacg
     2521 tgaattgcgg gctcgtcggg gtgatcagac gttcgaggtc agcctgcgcg aggtccgatt
     2581 gctcaaggtg ttccatgatc gcccaggcga ggtgctcgat cgcacgacgt tgttccgggc
     2641 cggctggggc gaagatcaca tcgcctcgag tcgtacgctc gaccagcaca tcgcccaact
     2701 gcgcaaacgc ctcgaaatcg accccagaaa cccgcgcatc atcacgaccg tgcacggggc
     2761 cggctatcgg tatgatctcg tgcccactga cccggacaaa cgtcggtgaa cgttatgagg
     2821 atgtcgtgcc gaagactggc gaacggccga gggcgattcc tcccgaggca gttcgaggtg
     2881 gcgacgtatg acgactgcgg ggacggtatg actgtcaccg ttcgaaaagc gtgagaagcg
     2941 tcaggagtgg tcgtcaagtt tggaggcgtc ggctggtgac ggtcggggga gcccgctgtg
     3001 ggagcgaatt tgatacaggt agccgatcac gggcaccaga atcgtggccc agagccactg
     3061 ccagttgttg ccgacgaaat cggagatctg gcgagtccag gtgacctgga tctcgatcgt
     3121 gcgttcgaac gtccgaatgg cacgtggaac tcgctgacca tcaacattca gaaccgctga
     3181 catggagagg tgcaggcgtt gagggccgcc ttctgtcggc tccacttccc acttccactc
     3241 ggtggtttcc acggcgctga ccgcttgcgt ttccggggtg atagcctgaa tcttgaatcc
     3301 tttgccggta agatgggctt ccatttcgct tcccacgcgg atctgcgccc cggtcttctg
     3361 tccctcggtc tcgatcaggg ttttcaggtt gtcgacggtt tctcgcgttg acaatgccag
     3421 atgaatgact tgggcttccc cgagtgggat ggtcgtcggt gtgttgaacg cgatgtttcc
     3481 cagggtgagc gaggacagca gtcggtcaat cgcttccatc gcggggtctg gcctcgaaag
     3541 ggtagctccg atgctgctga acatcgccct ctgttggtac gacgtgtagt acgggaccgc
     3601 taccagaacg acaagaacag ccaccaaacc aaacagaatc ttcttgccgc ggctcatgtt
     3661 tgttcctccc aaatcctccg gttgttccgg ttcttgtggt aatggtaaca cgattgccag
     3721 agtgtttggg cga
//
LOCUS       DAORHD010000353         3727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27194_length_3727_cov_3.822440, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000353 DAORHD010000000
VERSION     DAORHD010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27194_length_3727_cov_3.822440"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /locus_tag="PKO06_08610"
     CDS             complement(<1..1746)
                     /locus_tag="PKO06_08610"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV69744.1"
                     /translation="MSEAQQHAPPKGFFLHAARFLVTWRRSLFFGMVLLVICSLFFVK
                     NIKIDNSLEVWFLEGDPTLTGYREFKKQYGNDEVIVAVIDCEKQGGVFSHEMLARLYK
                     TSKDLEADTANFKRIVSVGVAPYIGLQGQDLIVEDLIATTPTRLEEAETIKKRFFENP
                     LWKKLLLEPSERYAIFIIEPIASDDMDKKRPEIIAAARKHLQTYAYKLAGMGVMYDEL
                     NRLSLRDGAIFSSVSYVIIAVLIYALYRSWVFFSIVVTVMLLGGASFMGIFGLFEQNF
                     NMVTIVLPTLMMILSISDVTYIINIYCFRIDKVVKDREEGLIEVFADCLSPCLFTSIT
                     NGMGFFSIMASPMEVLRGFGLLAGISCFLEYFVSVIAAAYILGRLIPDPQMRLQRPFE
                     GWMDGWVKMVPKYNRLVMLLLIATTGTGLYGISLLQVDTYSMGFLHESNPVRRDSDAI
                     ESTYGNYLPLEVRLITGKDDGVKDPAFLKRLEKAHTTLESIAGVERAASIIDVICRLN
                     QVWTDGASSSYRVPDEFNQVSQLLMMYESDPDNDLEHMVNRSYAEARLTVRLRMVSAS
                     WLRAYEEKVRVALEQL"
     gene            complement(1752..>3727)
                     /locus_tag="PKO06_08615"
     CDS             complement(1752..>3727)
                     /locus_tag="PKO06_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNV69745.1"
                     /translation="ACLTDEQIVQIAKVCHQIERTYGGKPQDIEWTIDADGTVRILQS
                     RPITTIDRPYPSDRAHTTVWDNSNIVESYSGVTTPLTFSFALYAYHQVYIQFCEVLGV
                     PKQDILEKDFNFGNMLGLLNGRIYYNLKNWYHLISVLPGYQYNSRFMEGMMGVKVKFD
                     FDPTIKKSTGGFFQKYFVELPRLAWVGANMAWKFYTTDTQVKEFMTIFQNMYDQYKDF
                     DFDNTSAQKLVAIYNELEYKVLRNWKAPIINDFMAMIFYGILRSLVSSWNLDKDGALA
                     NNLLAGQGNVESTLPLKALQRIARGIVSTPTLLETFKTHDPASLCRQFCKPPATTDTP
                     EQQKLCKEIYEYLNLYGYRSMNELKLEEPSLNEKPEFIFQMLKNYLTMPLGSDEEGGS
                     KEAAMREEAEKRVAELLAGQKIWGIIPKLSVLNFVTKYAKKAMAVREYQRFARTKMYG
                     LARRIFLGIGHRMQERGLLAAADDVFYLTKQEVIGYVVGTATSQKLSELAAIRRREFD
                     AYREMDELPDRIETKGMVYTNDLTQGVLKAQESTDPNLLKGLAACPGIVRKQVKVILT
                     PRDDMTLNGEILVAARTDPGWVPLYASASGLLIERGSMLSHSVIVARELGLPAIVGIT
                     DITKRVKTGDIVEMDGSTGIVRVIERLEASQKV"
BASE COUNT          810 a         1021 c         1047 g          849 t
ORIGIN      
        1 cagctgttcc agcgcgaccc gcactttctc ttcataggcg cgcagccacg aagcgctgac
       61 catgcgcagt cgaaccgtca ggcgggcttc ggcatacgac cggttaacca tgtgttcgag
      121 atcgttgtcg gggtctgact catacatcat caacaactga ctgacctgat tgaactcatc
      181 gggaacacgg taggaagatg atgcgccgtc agtccagacc tggttcagtc ggcagatgac
      241 gtcgatgatc gatgccgcgc gttcgactcc ggcaatgctt tcgagcgtgg tgtgcgcttt
      301 ttcgagacgt ttgaggaacg ccgggtcttt gaccccgtcg tccttgccgg tgatcaggcg
      361 aacttcgagc ggcagataat tgccataggt cgattcgatg gcgtcactgt cacgtcgtac
      421 cgggttactt tcatggagga agcccatgga ataggtgtcg acctgcaaca gggagattcc
      481 gtacaagccc gtaccggtgg tggcgatcaa cagaagcatc accagacggt tatactttgg
      541 caccattttg acccagccgt ccatccagcc ttcgaagggc cgctgcaggc gcatctgcgg
      601 gtctggaatc aggcggccaa gaatgtaggc agccgcgatg accgagacga aatactcgag
      661 aaaacagctg atgccggcca ggagtccgaa gccgcgcaga acttccatcg gactggccat
      721 gatcgagaag aaccccatgc cgttggtaat cgaggtaaac agacagggag acaggcagtc
      781 ggcgaagact tcgatcagtc cttcttcgcg gtccttcacg accttgtcga tgcgaaaaca
      841 gtaaatgttg atgatatagg tgacatctga aatgctcaga atcatcatca gcgtcggcag
      901 gacgatcgtc accatgttga agttctgttc gaacaagccg aagatgccca tgaacgaagc
      961 gccgccgaga agcatgaccg tcaccacgat gctgaaaaac acccacgatc gatacagggc
     1021 atagatcaac accgcgatga tgacatacga aacgctcgaa aagatggcac cgtcacgcag
     1081 actcaaccga ttgagctcat catacatgac gcccatgccg gcgagtttgt acgcatacgt
     1141 ttgcagatgt ttgcgtgcag cggcgatgat ctccgggcgc ttcttatcca tgtcatcaga
     1201 ggcaatgggc tcgatgatga agatggcata tcgctcggaa ggctccagca gcagtttttt
     1261 ccacagcggg ttctcgaaaa agcgcttctt gatcgtctca gcttcttcca ggcgcgtcgg
     1321 cgtcgttgca atgagatctt cgacgatgag atcctgcccc tgcaggccga tgtatggggc
     1381 cactccgaca ctgacaattc gcttgaagtt cgcggtgtct gcctcgaggt ctttcgaggt
     1441 cttgtacaaa cgcgccagca tctcgtgcga gaacaccccg ccctgcttct cgcaatcgat
     1501 gactgcgacg atcacctcgt cgttcccata ctgcttcttg aactcgcgat agcccgtcag
     1561 cgtcggatca ccctcgagaa accagacttc gaggctgttg tcgatcttga tgttcttgac
     1621 gaagaagagt gagcagatga ccagcagaac catgccgaag aacagacttc gccgccacgt
     1681 aacgagaaat cgcgcggcat gcagaaagaa gcccttcggc ggtgcgtgtt gttgcgcttc
     1741 actcattgct gttatacctt ctgagaagcc tcgagacgtt caatgactcg gactattccg
     1801 gtcgaaccat ccatctcgac aatgtcaccg gtcttcactc gcttcgtgat atccgtgatg
     1861 ccgacgatgg ccggcaggcc cagctcgcgg gcgacgatga cggaatgcga caacatgctg
     1921 ccgcgctcga tgagcaggcc cgaggccgag gcgtagagcg gtacccagcc ggggtcggtg
     1981 cgtgcggcca cgagaatctc gccattgagt gtcatgtcgt cgcgaggcgt cagaatcact
     2041 ttcacctgct tgcgcacgat gccggggcag gccgcgagac ctttcagcag attcggatcc
     2101 gtgctttctt gggctttcaa gacgccctgc gtcagatcgt tggtatacac catgcccttg
     2161 gtttcaatgc gatcaggcag ttcgtccatc tcacgatagg catcgaactc gcggcgtctg
     2221 atcgcagcga gctcagagag tttctgactg gtggcggttc cgacgacata accgatgact
     2281 tcctgcttcg tcagatagaa cacatcatcc gcggcggcca gcaagcctcg ttcctgcatg
     2341 cgatgaccga tgccgagaaa gatacgacgg gccagaccgt acatcttggt acgggcgaac
     2401 cgctggtatt ctcgcacggc catggctttc ttcgcatact tcgtgacgaa gttcagcact
     2461 gacagcttgg gaatgatgcc ccagatcttc tgcccggcca gaagctcggc gacgcgcttt
     2521 tcggcttcct cgcgcatagc ggcttccttg ctgcccccct cttcatcgct gccaagcggc
     2581 atggtgagat agttcttgag catctggaag atgaactcgg gtttttcgtt caaagagggt
     2641 tcttcgagtt tcagctcgtt catggaccgg tacccgtaca agttcagata ctcgtaaatc
     2701 tccttgcaca gcttctgctg ctcgggtgtg tcggtcgtgg ctggcggctt gcagaactgg
     2761 cggcacagcg aagccggatc atgagtcttg aaggtttcga gcagcgttgg cgtcgaaacg
     2821 atcccgcggg cgatacgctg cagtgctttc agcggcagcg tgctttcgac gtttccttgt
     2881 ccggcgagca gattattggc aagcgcgccg tctttgtcca gattccacga cgagacgaga
     2941 ctgcgcagaa tgccgtagaa gatcatggcc atgaaatcat tgatgatggg cgccttccag
     3001 ttgcgcagca ccttgtactc gagctcgttg tagatcgcga ccagtttctg tgccgaggtg
     3061 ttatcgaagt cgaagtcctt gtactgatcg tacatgttct gaaagatcgt catgaactcc
     3121 ttgacctggg tgtccgtggt atagaacttc caggccatgt tggcgccaac ccaggccagt
     3181 ctcggcagtt cgacgaaata tttctggaaa aaaccgccgg ttgatttctt gatcgtcggg
     3241 tcgaagtcga atttgacttt gacccccatc attccttcca tgaatcgaga gttgtactga
     3301 tatccgggta gtacggagat gaggtgatac cagtttttca ggttgtaata gatgcgcccg
     3361 ttcaacaacc cgagcatgtt cccgaaattg aagtccttct ccagaatgtc ctgtttcgga
     3421 accccgagaa cttcgcagaa ctgaatgtag acctgatgat aggcatagag cgcgaaggaa
     3481 aaggtcagcg gggtcgtgac tccggaatag ctttcgacga tgttcgagtt gtcccagacc
     3541 gtggtatgag cccggtccga gggatacggt cgatcaatcg tcgtgatggg gcgtgactgc
     3601 agaatgcgca ccgtgccgtc cgcgtcgatg gtccactcga tatcctgcgg tttgccgccg
     3661 taggtgcgtt cgatctggtg acagaccttg gcaatctgta cgatctgctc gtcggtgagg
     3721 catgctt
//
LOCUS       DAORHD010000354         3723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27250_length_3723_cov_3.338604, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000354 DAORHD010000000
VERSION     DAORHD010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27250_length_3723_cov_3.338604"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKO06_08620"
     CDS             complement(<1..181)
                     /locus_tag="PKO06_08620"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNV69746.1"
                     /translation="MGLCEKTCVPCKGGIPPLSREDAQNLVKQVPDWHLSETGTSISR
                     KFLFKTFMEAMEFANR"
     gene            complement(205..1788)
                     /locus_tag="PKO06_08625"
     CDS             complement(205..1788)
                     /locus_tag="PKO06_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV69747.1"
                     /translation="MLLNHRSALDILHGRLQALPGVASAVVICPADERSAAVSDWATK
                     QDLTCVVYEDAEARDKPRSLRQERWALEGDRGHDDLPGTVLARVAEREKAGLILVIPA
                     DHLAIDRESVQESLQLQFREDFDFLWSYDRLCGANWMILKAEMLLGLQKQHPEIMGYK
                     GGLVWALQKPLYPFRAGEYHTPRAQPSCHADLRDIAERMPAALAATGQIDETSDRSFR
                     YADWVGDVRWAKAYTDIGPQLVRAEPTNRCQGSCQACPHRLMQRPQVDLSGAVWQELV
                     SELKSTSSRWSFSGIGEPFLHQQFAQMVEDVRGRHVTVETSLQVDPPSAFPFEAVDLL
                     SISVDALEPTRFGRVREGCSWERIERFLAWNAQQKAAQPEHWPEVGVTMTKHQGTADL
                     DLSFLDYWKKVVTPVFRTDFFRWPIDELPKSVQWYQIRGSSDYLGFQPYPARTRYTPM
                     KRRPCRHALLGLHVLSDGRVTICPFDVDGRWILGNLQQQSPLEIWRSETARAFRAQHL
                     ERCWEERWPCARCQDWYHP"
     gene            complement(2031..2210)
                     /locus_tag="PKO06_08630"
     CDS             complement(2031..2210)
                     /locus_tag="PKO06_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69748.1"
                     /translation="MSSVGAVSSQSAVAEMMKLLSLQQEQSLDLAKKLIKVGAAEKVM
                     APMQEGLGENLDVMA"
     gene            complement(2260..2694)
                     /gene="fliS"
                     /locus_tag="PKO06_08635"
     CDS             complement(2260..2694)
                     /gene="fliS"
                     /locus_tag="PKO06_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009507990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNV69749.1"
                     /translation="MMPAGMQPDSYRKTQIDTASPESLILMLYDGALRFLGQAEDGFA
                     SNNTEVINNALLRVQAIVTELLTSLNKEKGGEIAVNLERLYLFFLDRLSEANIKKDPE
                     PMKQIRPLIQDLRDTWAEAMKQQVKNTGGKSMAPISRLNISA"
     gene            complement(2691..2981)
                     /locus_tag="PKO06_08640"
     CDS             complement(2691..2981)
                     /locus_tag="PKO06_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69750.1"
                     /translation="MRLHDRLCGASTLLNWFTIYQTKLVTRHTMQVRTLRQNENATVS
                     EWVTELLEFFDAVVRFHEYQLPLLQNAIDKLNNHIDKETGKPFETSSGGRAL"
     gene            complement(3014..>3723)
                     /locus_tag="PKO06_08645"
     CDS             complement(3014..>3723)
                     /locus_tag="PKO06_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV69751.1"
                     /translation="VEPMVHPDILESFRGPKMMMAFGGGIIPWIEKYREPFGNVICWG
                     SIATTAFDMARQFGCDPIIFLGLDLSFADGRLYARGSYSDDVFYDKVHHLTSLEHEIA
                     DYIGVRGVHRLTGPRGDVLFTDQNMNMYRQWFEDQFRSTKAKVINATEGGVVTRHVVC
                     QRFADAIRQYQDQGGPVRQLLSQALEAPVRLDREGLHAEMRTTLRELNEFRDVAREGI
                     GFVASWREANPRFRLAR"
BASE COUNT          831 a         1154 c          956 g          782 t
ORIGIN      
        1 cccgattggc aaactccatg gcttccatga acgtcttgaa caggaatttg cgactgatcg
       61 acgttcccgt ttcactcaaa tgccagtcag gaacctgttt caccagattc tgagcgtcct
      121 cccgcgacag tgggggaatg ccacccttgc agggcacgca ggttttttca cacaatccca
      181 tacatactcc ttgtcaaagc cgactcacgg atgataccag tcctggcacc tggcacatgg
      241 ccatcgttcc tcccagcatc gctccaggtg ctgtgcgcga aacgcgcgcg ccgtctcgga
      301 acgccagatt tcgagcgggc tctgttgctg caggttccct agaatccagc gaccgtcgac
      361 atcaaaaggg cagatcgtca cccggccatc actcagcaca tgcaacccaa gaagcgcatg
      421 gcgacaggga cgacgtttca tcggggtata tcgcgttctg gccgggtatg gttgaaaccc
      481 aagataatcg ctgctccctc gaatctgata ccattgcacc gatttcggca gttcatcgat
      541 gggccagcga aagaagtcgg tacggaacac cggcgtcacg acctttttcc agtagtcaag
      601 aaacgacaga tcgagatcgg cagtgccctg gtgtttggtc atcgtcacgc caacttccgg
      661 ccagtgttca ggttgcgcag ccttttgctg cgcattccag gccaggaaac gttcgatccg
      721 ttcccacgag cacccctcac gaacgcgacc aaaccgtgtc ggttccagcg catcaacgct
      781 gatactcagc aggtcgacgg cctcgaacgg aaatgccgac ggtggatcga cctgcaacga
      841 agtctcgacc gtcacatggc ggcctcgcac atcttcaacc atctgggcaa attgctgatg
      901 tagaaatggc tctccaatgc cggaaaatga ccatcggcta cttgtacttt tcaattcact
      961 aacaagttct tgccaaaccg cgccagacaa atcgacctga ggtctctgca tgagtcgatg
     1021 cgggcaagcc tggcaggacc cttgacaccg attggtcggc tccgcgcgaa ccagctgcgg
     1081 gccgatatcc gtgtaggcct tggcccagcg cacatcaccc acccaatcgg catagcgaaa
     1141 gctgcggtcg ctcgtttcgt caatctggcc cgtcgctgcc agcgcggcgg gcatgcgttc
     1201 cgcaatgtcg cgcagatcag catggcatga cggttgagct cgcggcgtat gatactcacc
     1261 cgcgcgaaaa gggtagagcg gcttctgcaa cgcccacacc agaccacctt tgtagcccat
     1321 gatttccgga tgctgcttct gcaggccaag aagcatttcc gccttcagga tcatccagtt
     1381 cgcgccgcag agacggtcat acgaccagag aaagtcgaag tcttcgcgaa actgcagctg
     1441 gagactctcc tgcacggatt ctcgatcgat cgccagatga tctgcgggaa tcaccagtat
     1501 cagaccggct ttttcccgtt ctgccactcg ggcaagaacg gttcccggca gatcgtcatg
     1561 cccacgatcc ccctccagag cccatcgttc ctgacgcaac gacctcggtt tgtcccgcgc
     1621 ttccgcgtct tcatagacga cacaggtcag gtcctgtttg gtggcccagt ctgagactgc
     1681 agccgagcgc tcgtcagcag ggcagatcac cacggctgac gccacgcccg gtaacgcctg
     1741 cagacggccg tgcaggatat ccagcgctga tcggtggttc agcaacaatc gggtaggatc
     1801 agggtccgag cgataggtcg ggcgtctacc cgggatatga tagatgagac aacaggatgg
     1861 aatgcgacac ctcgttttct ctagactgga ctcatgccag actcatcgaa gaatacagca
     1921 gcggcttgct ttttccaagc aacccgccga aaaaagaaac tgccccgcga acgaggcagt
     1981 gagagatccg ccctccatga caggagactg tcgcgaacac cgacgggcac tcaggccatg
     2041 acgtcgaggt tctcgccgag cccctcctgc atgggagcca tgaccttttc ggccgccccc
     2101 accttgatca gcttcttggc caggtccaga gactgctcct gctgcaggga cagcaacttc
     2161 atcatctctg ccacggcgct ctgagaactt accgcaccaa cgctggacat ggggcacctc
     2221 ctatctgctt ccgggcacgt acccgagagc attcttcact caggctgaaa tgttcagccg
     2281 cgaaattgga gccatggact tcccgcccgt attcttcacc tgctgtttca tcgcttcagc
     2341 ccaggtatcg cgcagatcct gaatcaatgg ccgaatctgc ttcatcggct ccggatcctt
     2401 cttgatgttc gcttcactca accgatcgag aaagaaaaga tacaaccgtt cgagattcac
     2461 cgcgatctct ccgcccttct ctttgttcag actggtcaga agttcggtca caatcgcctg
     2521 aacgcgcaac agcgcgttgt tgatcacctc cgtgttgtta ctggcaaagc cgtcttccgc
     2581 ctgcccgaga aaacgcaacg ccccgtcata caacatcaga atcagagact ccggagaagc
     2641 ggtgtcgatc tgggttttac gataggaatc aggttgcatg ccggcaggca tcataacgct
     2701 cgacctcctg aagatgtctc gaacggtttg cctgtttcct tatcgatatg gttgttcagc
     2761 ttatctatgg cattctgcag aagaggcaac tggtactcgt gaaaacgcac aacggcatca
     2821 aaaaactcca gcagttcggt cacccactca ctgacggttg cgttctcatt ctgtctcaga
     2881 gtgcgaacct gcatcgtgtg gcgcgtcacg agtttggtct gataaatcgt gaaccagttg
     2941 agaagcgtcg acgctccgca cagacggtca tgaagtcgca cggcatcgtc gaaccaggca
     3001 cgccattctc cgctcagcgt gccaatcgga agcgcgggtt cgcttcgcgc cagcttgcga
     3061 caaagcctat tccctctcgc gcgacgtcac gaaactcgtt caactctcgc agggtggtcc
     3121 gcatttcggc atgcaatccc tctcggtcaa ggcgaaccgg cgcttccaga gcctgcgaca
     3181 ataattgcct caccggaccg ccctggtcct gatactggcg aatggcatcg gcaaacctct
     3241 ggcacacgac gtgtcgcgtc accacgcccc cttccgtcgc gttgatgacc ttggctttcg
     3301 tacttctgaa ctggtcttcg aaccactgcc ggtacatatt catgttctga tcagtgaaca
     3361 ggacatcgcc ccgcggaccc gtcagacgat gcacgccacg aaccccgata tagtcggcaa
     3421 tttcgtgttc gagggaagtc agatggtgca ccttgtcata gaacacgtcg tcggagtagg
     3481 agccacgcgc atacaaacga ccatccgcaa acgaaagatc aaggccgaga aagatgatcg
     3541 gatcgcatcc gaactgtctg gccatgtcaa aagccgtcgt cgcgatcgag ccccagcaga
     3601 tgacattgcc gaaaggttcc cggtactttt cgatccatgg aatgatcccg ccaccgaatg
     3661 ccatcatcat cttcggcccg cggaaacttt cgagaatgtc cggatgtacc atgggttcaa
     3721 cag
//
LOCUS       DAORHD010000355         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27312_length_3719_cov_3.535753, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000355 DAORHD010000000
VERSION     DAORHD010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27312_length_3719_cov_3.535753"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PKO06_08650"
     CDS             complement(<1..426)
                     /locus_tag="PKO06_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69752.1"
                     /translation="MTASGNRTPILAHLPDLLLTVAFLGLTLATLLDTGGAAALAQEL
                     GYWLVYEFVGLFFLMFWAGLNEGRGDHPLDERFRVISTCAIYAASGLLMHALWAIPSS
                     IVLVSSAGGIWALVNPFLREDRPTPFRQGQETALAFVSLL"
     gene            680..1144
                     /locus_tag="PKO06_08655"
     CDS             680..1144
                     /locus_tag="PKO06_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69753.1"
                     /translation="MTDRTLNPKDVVYIGIGEDVDRMPEVQKTTKYWVTAASCAACGC
                     SGRLLVPEGDRLILRCVDCGSIGDAGLEARHLPEITFVYWCRFARFPSDQVELSQDQR
                     AEIERLIRELGPEPETPYYAMIITSGLCLHAFHCLAQGCPYRASPLMLDRLC"
     gene            complement(1220..2428)
                     /locus_tag="PKO06_08660"
     CDS             complement(1220..2428)
                     /locus_tag="PKO06_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV69754.1"
                     /translation="MIFSLAEVLLLCMLVDWLFRACRLPGFVGMLLVGFTLGPGGLNR
                     LDPSLLHIGIDLRILALMVIVLRAGFALRPSVIRALGLPLLFLCFVPSLIEAAIISTL
                     SRHLLGFTWPEALMLGFIIPAVSPAVVVPLMIQYIEEKRGHTHRVPSLVLAASSLDNI
                     FNVLMADLMIRQYAQPSSVLPSSLASIPLSLGLGLLLGFLLGQLLIRLFEIYNPRATK
                     RTLLVVTGGLLLYRLEKVMAGSLSFSALLAVLVMGCVILERRERFAREISAKLNKIWI
                     FAEIALFSVVGAQFNLTAALSTGLTGILLIVTGLAGRISAVFACLAGALPWGERLFAA
                     AAFVPKASVQAALAGIPLSVMISMQVNPEPGEKILALSVLSILLTAPLGAWLTRTTAE
                     RFLPPEQDVV"
     regulatory      complement(2470..2533)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2803..3171
                     /locus_tag="PKO06_08665"
     CDS             2803..3171
                     /locus_tag="PKO06_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69755.1"
                     /translation="MAKILLVDDDKDFVEINKTLLEKDKHTVVFAHNPKDGLALLKKE
                     KPDLLVLDVMMEEPDDGFAMAQQIRQDGNKIPIIMLTSINKVMNLKFGKDKDMVPVNE
                     FFEKPIPADKLREAVKKYVK"
     gene            3185..3694
                     /gene="nuoE"
                     /locus_tag="PKO06_08670"
     CDS             3185..3694
                     /gene="nuoE"
                     /locus_tag="PKO06_08670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV69756.1"
                     /translation="MLATELDQLRTEIKEIAKQYGGRREGLMPVLQHIQNKHRYVSPH
                     AMQEVAAEFGIHPVEVHGVVSFYSFLSTEKKGKFIIRLCQTISCDMAGKVAVARQLEN
                     ELGIKFGQTTKDGMFTLEYTNCLGMCDQGPAMLVNTEVYTKVTPESVSEVIATYMKKF
                     GAHVTQEVV"
BASE COUNT          859 a         1022 c         1160 g          678 t
ORIGIN      
        1 caacagcgag acgaaagcca gagcggtctc ttgcccctgc cggaagggtg tcggccggtc
       61 ttcgcggaga aatgggttga ccagcgccca gatgccgccg gcgctcgata cgagaacgat
      121 cgaagagggg attgcccaca gtgcatgcat gagcaggccc gacgccgcgt agatcgcaca
      181 ggtgctgatg acccggaacc tctcgtcaag cggatggtcg cctcgccctt cgttcagacc
      241 ggcccagaac atgagaaaga acaaaccgac aaactcgtat accagccagt atccgagttc
      301 ctgggccagg gctgcggccc cgcccgtgtc gagcagcgtg gcgagcgtga ggccgagaaa
      361 ggcgaccgtg agcagaaggt cgggcagatg agcgagaatg ggagtgcgat ttcctgatgc
      421 ggtcacgaaa aggctcctct ctcgactttc ttcgtcctca gaaaacgtgt atcctgagcc
      481 agtatgccag agtgcgcccg ttaccgtcaa tccgagaccc ggcgatgaac caggacgggt
      541 tgcctttgat gctgatggaa ccgcgcggtt tccgagagtg tgcgtccgag gtgatgcagg
      601 cttgtgtgag cattggtagc tgtattccgg tgtgtcgctc cccaggattt cctatccgtc
      661 cggcggggag agaacgatcg tgactgaccg aaccctgaac ccgaaagatg tcgtctacat
      721 cggcattggc gaggatgtgg accgcatgcc ggaggtgcag aaaacgacga aatactgggt
      781 gacggccgcg tcctgcgcgg cgtgcgggtg ttccggccgc ctgctggtac ccgagggaga
      841 ccgactgata ctgcgctgtg tcgattgcgg cagtatcggc gacgcggggc tcgaagcccg
      901 tcatctgccg gagatcacct tcgtgtactg gtgccgcttt gcccgcttcc catcggatca
      961 ggtcgaactg tcgcaagacc agagagccga gatcgagcgc ctgatccggg agctgggccc
     1021 tgagcccgag acgccctact acgccatgat catcacgtcg ggtctttgcc tgcacgcctt
     1081 tcattgtctg gcacagggct gtccctatcg cgcttctccg ctgatgctcg atcggctctg
     1141 ctgaagcagg acctgcgaga gccggcttgc ggatagatcg cgagaatgac gaggttcctg
     1201 gcgtttgctt ggcgggggct cagacgacat cctgttccgg tggcagaaag cgttcggcgg
     1261 ttgtgcgcgt caaccacgct ccgagcgggg cggtcagcag aatgctcagt accgacaagg
     1321 ccaggatctt ttcgccaggt tcaggattca cctgcatgct tatcatcacc gagagcggaa
     1381 ttccggcgag agcggcctgg acggatgcct tgggcacgaa cgcggcggcg gcgaagaggc
     1441 gttcgcccca gggcagcgca ccggccaggc aggcaaaaac ggctgagatt cgtccggcca
     1501 gaccggtgac gatgagcagg attccggtca gaccggtgct cagggcggcc gtcaggttga
     1561 attgcgctcc gacgacggaa aacagcgcaa tctcggcgaa gatccagatc ttgttcagct
     1621 tggccgagat ctctcgcgca aagcgttcac gccgttcgag aatgacgcac cccatgacca
     1681 gaacggcaag cagtgcagaa aagctcagag agccggccat aactttttcc aatcggtaca
     1741 acagaagccc accggtgacg accagcaggg tgcgcttggt cgcgcgcggg ttgtagattt
     1801 cgaacagccg gatcaggagc tgcccaagca gaaaccccag cagcaggccg agcccgagcg
     1861 acagcggaat ggaagccaaa gagctcggga gtaccgacga cggttgcgca tactgacgaa
     1921 tcatcaggtc agccatgagc acgttgaaga tattgtccag gctacttgct gccagaacca
     1981 ggctgggaac gcgatgagtg tgcccacgtt tttcttctat gtactgaatc atcagcggga
     2041 cgacgacggc gggcgagacc gcgggaatga tgaagccgag catcagagct tcgggccagg
     2101 tgaagccgag cagatggcgt gacagggtgc tgatgatcgc cgcctcgatg agcgacggaa
     2161 cgaaacagag aaacaagagc ggaaggccga gcgcgcggat gacggaggga cgcagggcga
     2221 agccggcgcg cagaacgatg accatcagag ccagaatgcg caggtcgatg ccgatgtgca
     2281 gaagagatgg atcgagacgg ttcaggccgc cggggccgag cgtaaagccg accagcagca
     2341 tgcctacgaa cccgggcagg cggcaggcgc gaaaaagcca gtcgaccagc atgcacagca
     2401 ggagtacttc cgccagactg aaaatcatca atggttcctc ccgggggaga acgagtcgtc
     2461 gccggctcgc gatatacagg agtcatcagc ctggacaggc ggttatgagg gggactccat
     2521 ccccgtcgcg ttgccactgt atcacgaacg ggccgcgttg acgagcgtgg caggacggac
     2581 cagggtcatt tcattcttca gactgggttt tcccgttggt atcgatttac tgaagcgctc
     2641 agcgcttgca ccacagcgtg tacgtcgacg gtacataagt gagaagcgtc tgttcatcag
     2701 tgattggcgg gccacgaatg ttcgcctcgc gaaagattga gaaaaatttc tcagactgtg
     2761 ctatagtcgg tttgcgaaaa ttttccttag gaggaacgcg gtatggccaa gatcctgttg
     2821 gttgatgacg acaaggattt tgtcgaaatc aacaagaccc tcctcgagaa ggacaagcac
     2881 acggtcgtgt ttgcccacaa cccgaaggac ggtctggcgc ttttaaagaa ggaaaaaccc
     2941 gatctgctgg ttctcgatgt catgatggaa gagcctgatg atggctttgc catggctcag
     3001 cagattcgcc aggacgggaa caagattccc atcatcatgt tgacctcgat caacaaagtg
     3061 atgaacctga agtttggcaa agacaaggac atggtgccgg tcaacgaatt ttttgagaag
     3121 cccattcccg ctgacaagct gcgtgaagca gtgaagaagt acgtcaagta aggcaggagg
     3181 aaccatgtta gccaccgaat tggatcaact gcgcacggaa atcaaggaga tcgcgaagca
     3241 gtacggcggc cgccgagaag gcctgatgcc cgttctccag cacattcaga acaagcaccg
     3301 ctacgtttct ccgcacgcga tgcaggaagt cgccgccgaa ttcggcattc acccggtcga
     3361 agttcacggc gtcgtcagct tctattcgtt cctttcgacc gaaaagaagg gcaagttcat
     3421 cattcgtctc tgccagacga tcagctgcga catggccggc aaggtcgccg tggctcgcca
     3481 gctcgagaac gagctcggta tcaagttcgg ccagaccacg aaagacggca tgttcactct
     3541 ggaatacacc aactgcctcg gcatgtgtga tcagggcccg gccatgctgg ttaacaccga
     3601 agtgtacacc aaggtgaccc cggaatccgt ttcggaagtc atcgcgacgt acatgaagaa
     3661 gttcggcgct cacgtcaccc aggaggttgt ctgatcatgt cgaagaaaga actggtgat
//
LOCUS       DAORHD010000356         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27346_length_3716_cov_3.226714, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000356 DAORHD010000000
VERSION     DAORHD010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27346_length_3716_cov_3.226714"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PKO06_08675"
     CDS             <1..1444
                     /locus_tag="PKO06_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV69757.1"
                     /translation="VGNKLTVAMVDPLNIIAVDDIQLMTGLMVKPVVATQSDINKALQ
                     EAYGAVAQQDKLMGELDDIGKEGEGADDLDQLGELGENDAPIIRLCNLVISQAVQNGV
                     SDIHIEPFEKELRIRYRQDGMLFTAMTPPKKATAAITSRIKIMSSLNIAEKRLPQDGR
                     IKIKVNNRSIDLRVSVLPVIWGEKIVMRILDQSSLRVNLEDLGFEPESLKRFKGSISA
                     PYGIALVTGPTGSGKTTTLYSALTSLNTIERNIMTAEDPVEYMLQGINQVQCRPEIGL
                     NFASALRAFLRQDPNIIMVGEIRDFETAEIAIKASMTGHLVLSTLHTNDAPGSVGRII
                     NMGIEPFMVTTSVLMIQAQRLVRKVCKDCKFEIKPRAEQISQFGITPELLRRLELPHI
                     NEKNMFFYKGKGCETCNNAGSKGRIGVYEIMVMTERLRDIILNGGSTDDIRKQAIDDG
                     MLSLRESALRKALLGVTSIEEVVRVTLGEH"
     gene            1514..2575
                     /locus_tag="PKO06_08680"
     CDS             1514..2575
                     /locus_tag="PKO06_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV69758.1"
                     /translation="MFVLNDLLNLVIENGASDLHITVGSPPVIRVDGELVQTDLDVLT
                     PMDTRSLIYNMLTAEQQKEFEEKLELDISYSVHGFGRFRVNVYKQRGCIGSAFRVIPT
                     RIPTLEELRLPAKLREFTKLNKGLVLVTGPTGSGKSTTLASLINIINEEQRVHIVTIE
                     DPIEYLHYHKKAVVNQRELHIDTHSFTEALRHVLRQDPDVVLVGEMRDLETVGTALTL
                     SETGHLVFGTLHTTDAAQTINRIIDIFPPHQQGQIRSQLSFSLRAVVAQMLMPTASGK
                     GRVCATEILTGNPAVANCIREQKISSIYTIMQTSQNEGMMTFEQSLRDLYMRGDITLE
                     EALQHTMHPKELERMLKGG"
     gene            2665..>3716
                     /locus_tag="PKO06_08685"
     CDS             2665..>3716
                     /locus_tag="PKO06_08685"
                     /inference="COORDINATES: protein motif:HMM:NF021635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HNV69759.1"
                     /translation="MNRFGNDGESWCGNQGKTVMTRALLGGAILLAYGWFFFGVEIYA
                     AFDTVFHLYFAREFYEALLESLTWPDWDAFPYDGRGTPAFRFYAPVGYLISAALQLLG
                     APVQVALKGTILLFAVVGAIGVYYWFRRLGLRRERPLGAALFLSSPFLAVHLTRVFFF
                     QNLLAILLFPWVCYGLAHRGRGRKRGGIIAGVTLGVMALTHLPATLMAGYLSVVILTL
                     TRHGRETWSQVLRELLLIVALAAGIAAPYLLPAMAERTTVNFHSHMAIQEIGKPGDFL
                     DDRRIDDLASATASFSGASEFFAPQLHARFRLLLLCVLIGSIPLWTVPATKLSRRFWF
                     CGLIPLPLLVRASLPI"
BASE COUNT          798 a         1005 c         1046 g          867 t
ORIGIN      
        1 ggtcggaaac aagctgaccg tggctatggt cgacccgctg aacatcatcg cggtcgacga
       61 tattcagctg atgaccggcc tcatggtcaa gccggtcgtc gcgacgcaga gcgacattaa
      121 caaagctctg caggaagcct atggcgcggt tgcccagcag gacaaactga tgggtgaact
      181 cgatgatatc ggcaaggaag gcgaaggagc cgacgatctc gatcaactcg gcgaacttgg
      241 tgaaaacgac gcaccgatca ttcgactctg taacctcgtc atatcgcagg ctgtgcaaaa
      301 tggcgtctcg gacattcaca ttgaaccgtt cgaaaaggaa ctgcgcattc gctaccgcca
      361 ggacggtatg ttgttcaccg ccatgacgcc gccgaaaaaa gcgacggccg cgatcacgtc
      421 gcgcatcaag atcatgtcgt cattgaacat cgccgaaaag cgtctgccgc aggacggccg
      481 catcaagatc aaggtgaaca atcgctcgat tgacttgcgc gtctccgtgc ttcccgtcat
      541 ctggggcgaa aagatcgtta tgcgtattct cgatcagagt tcactgcgcg tgaacctcga
      601 agatcttggt ttcgagccgg aatcgctgaa acgtttcaag ggatccattt ccgcgccgta
      661 tggcattgcg ctggtcaccg gtccgacggg gtcgggcaag accacgaccc tgtactcggc
      721 gttgacatcg ctaaatacta tcgaacgcaa catcatgacg gccgaagacc ccgtcgaata
      781 catgctgcag ggtatcaacc aggtgcagtg ccgaccggaa atcggcctga acttcgcatc
      841 agccctgcgt gcctttctgc gacaagatcc gaacatcatc atggtcggtg agattcgtga
      901 ctttgaaacg gcagaaatcg ccatcaaagc gtcgatgact ggccacctcg tgctgtcaac
      961 gctgcacacc aacgacgcgc ctggttccgt tggtcgtatc attaacatgg gtatcgagcc
     1021 attcatggtc acgacgtccg tgttgatgat tcaggctcag cgtctcgtgc gcaaagtctg
     1081 taaagactgc aagtttgaga tcaagccccg cgcggaacag atctcgcagt ttggtattac
     1141 gcccgaactc ctgcgccgtc tcgaactgcc gcatatcaac gaaaagaaca tgttctttta
     1201 caagggcaaa ggttgtgaaa cgtgcaataa cgccgggtcg aaaggccgca tcggcgtcta
     1261 tgagatcatg gtcatgacgg aacgtctacg agacatcatt ctgaacggtg gttcgaccga
     1321 cgatatccgc aagcaggcca ttgacgacgg catgttatcg ttgcgtgaat cggcgctgcg
     1381 caaagcgttg ctcggcgtga cctcgatcga agaagtcgtt cgcgtgaccc tcggcgaaca
     1441 ctaattcgcc tgttacgaat tgtacaattt agaaatagac aaggcgccct tggggtgcgg
     1501 gagttggtga aacatgttcg tattgaatga tctcttgaat ctggtcatcg aaaacggcgc
     1561 atcagacctt catatcaccg tcggcagtcc acctgtcatt cgcgtcgacg gcgaactggt
     1621 acaaaccgac ctcgatgttc tgacccccat ggatacgcgg tcactgattt ataacatgct
     1681 cacggcggag caacagaaag agttcgagga aaagctggaa ctcgacattt cttattccgt
     1741 gcatggtttt ggccgctttc gtgtcaacgt ttacaaacaa cgcggatgca tcggatcagc
     1801 attccgcgtc attccgaccc gcattccgac gctcgaagaa ctgcggttgc cggcgaagtt
     1861 gcgtgagttc acgaagctga ataaagggct ggttctggtg accggtccga ccggctccgg
     1921 aaagtcgacc acactggctt cactgatcaa tatcatcaat gaagaacagc gcgtgcatat
     1981 cgtcaccatc gaagatccga tcgaatatct gcattatcac aagaaagcgg tggtgaacca
     2041 gcgcgaactg cacatcgaca cgcactcgtt taccgaggcc ttgcgccacg tgctgcgcca
     2101 ggaccccgat gtcgttctcg tcggcgaaat gcgcgatctc gaaaccgtcg gtacggctct
     2161 gacgctgtca gaaaccggtc acttggtgtt cggcaccttg cacacgaccg atgcggcgca
     2221 gacgatcaac cgcatcatcg atatctttcc cccgcatcaa caggggcaga tccgatcgca
     2281 gctgtccttt tcattgcgcg cagttgtggc ccagatgctg atgcctactg ccagcggcaa
     2341 ggggcgtgtc tgcgccaccg aaatcctgac gggcaatccg gccgtcgcga actgtattcg
     2401 tgaacagaag atatcgagca tctatacgat catgcagacg agccagaacg aaggcatgat
     2461 gaccttcgaa caatcgttgc gcgacctgta catgcgtggc gacatcacac tcgaagaagc
     2521 gctgcagcac acgatgcacc cgaaagaact cgagcgcatg ctcaagggtg gctgacgccc
     2581 tcttcctgtt ctgccttact tgatcgtacc ggacggttcg cgcgcgtagg ctcatctgtt
     2641 gcttattatg gatgagacga aagtgtgaat cggttcggta atgatggaga aagctggtgt
     2701 ggtaatcagg gaaaaaccgt gatgacacga gccctgctgg gaggggcgat tctcctggcg
     2761 tacgggtggt ttttcttcgg cgttgagatc tacgcggcgt ttgatacggt ttttcatctg
     2821 tacttcgctc gtgaattcta cgaggccctt ctcgaaagtc tgacctggcc agactgggat
     2881 gcgttcccgt atgatggtcg aggaacgcct gcgtttcgtt tttatgcccc ggtgggatat
     2941 ctgatcagtg cggctttgca gcttctcggg gctccggtgc aggtggccct gaagggaacg
     3001 attctgctgt tcgccgttgt cggagcgatc ggtgtctatt actggtttcg acgcctgggt
     3061 ctgaggcgtg aacgccctct gggggcggct ctcttcctgt catctccttt cctggcggtt
     3121 cacctgacgc gggttttctt ttttcagaac ctgctcgcga ttctgttgtt tccgtgggtg
     3181 tgctacgggt tggcgcaccg tgggcgaggc cgaaaacgtg gtggcatcat cgctggagtg
     3241 actctgggag tcatggcgct tacacatctg ccggcgacgc tgatggctgg gtatctgtcc
     3301 gttgtcatcc ttaccctgac acgtcatggg cgtgagacct ggagccaggt gctgcgtgag
     3361 ttgttgttga ttgtggcgtt ggctgcagga atcgctgcgc cctatctgct tccggcgatg
     3421 gccgaacgga cgacggttaa ctttcacagt cacatggcga tccaggagat tgggaagccc
     3481 ggagattttc tggatgatcg gcgaatcgat gacctggcgt cggcaactgc ttcgtttagc
     3541 ggtgcttctg agttttttgc gccccagttg catgcacgat ttcggctctt gctgctctgt
     3601 gtcctgatcg ggtcgatccc tctctggaca gtaccagcga caaaattgtc tcgacgtttc
     3661 tggttctgtg gtctcatccc actccccttg ttggtacggg cttcactgcc gatttg
//
LOCUS       DAORHD010000357         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27461_length_3705_cov_3.167123, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000357 DAORHD010000000
VERSION     DAORHD010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27461_length_3705_cov_3.167123"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKO06_08690"
     CDS             <1..296
                     /locus_tag="PKO06_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69760.1"
                     /translation="TERQREDDLRRRAPAPAVPALPAPGEFMPGPGEEPPAQGTEAGL
                     FDEPTPALRREGKPLKLGSITDAVYEEGRRFLRELRQKQSQNQNRPEPRKNED"
     gene            286..504
                     /locus_tag="PKO06_08695"
     CDS             286..504
                     /locus_tag="PKO06_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69761.1"
                     /translation="MKTEPRDLPFDLLVDQVYQRLLAAEPQDARAVLDALVAEQSGWA
                     NRCAVESAIRQLLVTEHLRLKARLHERT"
     gene            666..920
                     /locus_tag="PKO06_08700"
     CDS             666..920
                     /locus_tag="PKO06_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69762.1"
                     /translation="MKVSEEIKTVATWLRSYPTDQKNIEAMAKRLEAIADHTVIPNEA
                     KIEHLALQLEDAVQSGDAFYARSQAALAVGTLRAIARGQS"
     gene            917..1147
                     /locus_tag="PKO06_08705"
     CDS             917..1147
                     /locus_tag="PKO06_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV69763.1"
                     /translation="MKKTVETVRFDPGSFTSFELADRLSISQAGASYLLRGLLKLGQV
                     KRSREGRKFRYALCPSAPAMVEYNKLYAQEAD"
     gene            1149..1493
                     /locus_tag="PKO06_08710"
     CDS             1149..1493
                     /locus_tag="PKO06_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69764.1"
                     /translation="MNRSELKDVLLMAALAGALVWTGVKVVRVRDDVVRLRAHEIGCP
                     YTESFFRENLTTDWAQGKVTYEFWYCDGKAVKARSTQPDPRTPGQVLVLEITEKDIPA
                     IEKTLKKVEVKP"
     gene            1490..1720
                     /locus_tag="PKO06_08715"
     CDS             1490..1720
                     /locus_tag="PKO06_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69765.1"
                     /translation="MRLNRNGHGMQGLAELAMIMIALAPVFWPLFFLRDGDPAWWTAL
                     CVIGQIAWLYLIFCGLRGLWRAFVQWAGGKKP"
     gene            1717..1980
                     /locus_tag="PKO06_08720"
     CDS             1717..1980
                     /locus_tag="PKO06_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69766.1"
                     /translation="MRRPLDAVVRRLEKKAVRAVQEWNARYKKGQRVVVAVGDERFES
                     MTSSRARISQTDPTQALIWLSGMSIAFDLSAITPIKCLPSRQK"
     gene            2261..2494
                     /locus_tag="PKO06_08725"
     CDS             2261..2494
                     /locus_tag="PKO06_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69767.1"
                     /translation="MIVSLPCEFERWMIGMVARFPKALPQRKVGQEMKTLHDVTNVLM
                     IMSLAGQALALTVWAMAGVCMMIEIIVKCLRGA"
     gene            2779..3465
                     /locus_tag="PKO06_08730"
     CDS             2779..3465
                     /locus_tag="PKO06_08730"
                     /inference="COORDINATES: protein motif:HMM:NF022022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HNV69768.1"
                     /translation="MTSQIQSQLLPVSFHGDTLFVVSHEGEPFTPARPIVQGMGIRWQ
                     SQQRKLAQAKQRWGVIMMMTPTKTGPQATLCIPVRKLAAFLATIDPRRVRPEIRAKIE
                     LYQAECDDALWSYWTKGEALNPRRFEAETKVRTPEPVRQIPAARPLPPETTAISNREY
                     VELLKTKIAFLEMRGRTKTTRQSPRPVTSADAAEIRQLHSSGFTKGAIARRLNRSTST
                     VCKVLKGAIA"
     gene            3462..3656
                     /locus_tag="PKO06_08735"
     CDS             3462..3656
                     /locus_tag="PKO06_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69769.1"
                     /translation="MKATALRNAMDEVERVSSALRAVRDMVCTGDDLHTVKPDDLSTL
                     LDILLEKLQSAVTAAESSFT"
BASE COUNT          834 a         1035 c         1150 g          686 t
ORIGIN      
        1 agacggaacg acagcgcgag gacgatctgc gccgtcgggc tccggccccg gcagtgccgg
       61 ctctgccggc gccgggcgaa ttcatgcccg gcccgggcga agagccgccc gcacagggca
      121 ccgaagcggg gttgttcgat gagccaaccc cggctctacg ccgtgaaggc aagccgctga
      181 agcttggaag catcaccgat gcggtctacg aagaaggccg tcgatttctg cgcgaactca
      241 ggcagaagca gagccagaac cagaacagac cggagccccg gaagaatgaa gactgaaccg
      301 cgcgatctgc cgttcgacct gctcgtcgat caggtctacc agcgcctgct ggccgcagag
      361 ccgcaggatg cgcgggccgt gctcgatgcc ctggtcgcgg agcaaagcgg ctgggcgaac
      421 cggtgcgcgg tcgaatcggc gatacggcag ctgctggtga ccgagcatct gcggttgaaa
      481 gcgaggctgc atgagcgaac ctgatgaatc agacgtgacc gaaaaacgcg cggagcaggt
      541 cgtgagcacg gtcaacgcct atttgaaagc gatgttgagc ggcgacacgc gagcgatgaa
      601 ccgaacagga gaagcatggg atgccctctg tggagtgtgg caccccggac atacaggagg
      661 cagtcatgaa ggtaagtgaa gaaatcaaga cggtggcgac ctggttgcgg tcttacccga
      721 ccgatcagaa gaacatcgaa gcgatggcga agcggctcga agcgatcgcg gatcatacgg
      781 tgattccgaa cgaagcgaag atcgaacacc tggcgttgca actcgaagac gcagtgcaga
      841 gcggcgatgc gttctatgcg cgcagccagg cggccctggc ggtcggaacg ctgcgcgcga
      901 tcgcgcgggg gcagtcatga aaaagaccgt tgagaccgtg agattcgacc ccggttcgtt
      961 cacgtcgttc gagctggccg accggttgag catatctcag gcaggcgcat cgtatctgct
     1021 tcgcggtctt ctcaaactcg gacaagtgaa gcggtcgcgc gaggggcgga agtttcgcta
     1081 cgcgctttgc ccgtcggcac cggcgatggt ggaatacaac aagctctacg cccaggaggc
     1141 cgactgacat gaaccgaagc gagttgaaag acgtgctgct gatggcggct ttggccgggg
     1201 cgctcgtctg gaccggcgtg aaggtcgttc gcgtgagaga cgacgtcgtc cgcctgcggg
     1261 cgcatgagat cggctgcccc tacaccgaat cgttcttccg agagaacctg accacagact
     1321 gggcgcaggg caaggtaacg tatgagttct ggtattgcga tggcaaggcg gtgaaggctc
     1381 gatcgacgca gccggacccg agaacgcctg gtcaggttct tgtgttggag atcacggaga
     1441 aagacatccc ggcaatcgaa aaaacactga agaaggtgga agtaaagcca tgagactgaa
     1501 tcgaaacggg catggaatgc agggcctcgc ggagctggcc atgatcatga tcgcgctggc
     1561 tccggttttc tggccgctct tttttcttcg cgacggcgac cccgcctggt ggacagctct
     1621 atgtgtcatc ggccagatcg cctggctgta tctcatcttc tgcggcctgc gcggcctgtg
     1681 gcgtgcattc gtgcagtggg caggagggaa aaagccatga gacgaccgct tgatgccgtg
     1741 gttcgaaggc tcgaaaagaa ggctgtccga gccgttcaag agtggaatgc gcgttacaag
     1801 aagggtcagc gggtagtagt cgcggtcggt gacgagcgtt tcgagagcat gaccagcagc
     1861 cgtgcgcgga tctcgcagac cgacccgact caggccctca tctggctgag cggcatgagt
     1921 atcgccttcg acctctcggc cataaccccg atcaaatgcc tgccttcaag gcagaaataa
     1981 ggagaaccga aatgaactac tggaagaatg tgatgccacc agtgggctgg gaatgccgat
     2041 gcatgcctac ttgtcaatcc cttggagact ctgagtcatt gaggcaagat actcccccgc
     2101 gcccgattcg ccaatcttcc gaagttggca tgtcgcccca tccagttgaa ccatcagatg
     2161 atccagcttg agggaaccgt ttttgcatag cgctctgacc aaggccgtat atgcgtaaca
     2221 aaacgccaga gacttttcat cggccggagt gaagtcagtc atgattgttt cccttccgtg
     2281 tgaattcgaa cgatggatga tcggcatggt tgcaagattt ccgaaggctt tgcctcaaag
     2341 aaaggtagga caagaaatga aaactctgca tgatgttacg aacgtcttga tgatcatgtc
     2401 gctggcgggg caggctcttg cactgactgt atgggccatg gctggagtct gtatgatgat
     2461 cgaaatcatc gtgaaatgcc tgcgcggcgc ataggagact aggacaatcg agaataggca
     2521 gagtcacgag tgatgaggat ttgaggggga aatttgacat gaggggcgaa agtagatatg
     2581 ctgagtgcag gtgccactaa caccttagcc gtagcggtct ccgccccgaa gtcgcggatt
     2641 tttgtttttc ggaaacattt gtcgggtgtc gacaggaata caacaccttt cggggaaata
     2701 ctgtcggccg tctacggcgg ttagtaacgc ccggccccca ttttcggggg gtcactaaaa
     2761 ccgtaggagg tcatttttat gaccagccag attcagtccc agcttcttcc cgtttcgttt
     2821 cacggtgaca cgctgttcgt cgtgtctcac gagggtgagc cattcacacc ggctcgacct
     2881 atcgtccaag gcatggggat cagatggcaa tcacaacagc gcaaacttgc tcaggctaag
     2941 cagcgctggg gtgtcatcat gatgatgaca cctacgaaaa ctgggcctca agccactctg
     3001 tgcataccgg tccgaaaact ggcagcattc ctggcgacga tcgacccccg cagggtccgc
     3061 ccggagatcc gcgcgaagat cgaactgtat caggccgagt gcgatgacgc gttgtggtcc
     3121 tactggacga agggcgaagc gttgaacccg cgccggtttg aggcagagac gaaggttcgg
     3181 actcccgagc cggttcgcca gattccggcc gcacgtccgc tgccgccgga gacgaccgcc
     3241 atctcgaacc gcgaatacgt cgagctgctg aaaaccaaga tcgcctttct cgaaatgaga
     3301 ggccgcacaa agaccacgcg ccaatcgccc cggcccgtga cttctgccga tgccgccgag
     3361 atcagacaac tgcattcgag cggattcaca aagggcgcga tcgcccgccg cctgaatcgc
     3421 agcacgtcaa ccgtgtgcaa ggttctgaaa ggggccatag catgaaggca accgccctgc
     3481 gaaacgccat ggacgaagtg gagcgcgtgt catccgcgtt gcgtgccgtt cgagacatgg
     3541 tctgcacggg cgacgatctg cataccgtaa agcccgacga cctctcgacg ctcctcgaca
     3601 tccttctcga gaaactgcaa tcagccgtca ccgccgctga gtcatccttc acctgacccg
     3661 cccgcagcac aacggcccgg agcctgcgcc ccgggccgtt ttctt
//
LOCUS       DAORHD010000358         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27462_length_3705_cov_2.815068, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000358 DAORHD010000000
VERSION     DAORHD010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27462_length_3705_cov_2.815068"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1698
                     /locus_tag="PKO06_08740"
     CDS             253..1698
                     /locus_tag="PKO06_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69770.1"
                     /translation="MTLRVALVNPPFLPDYSRGQRSPAVTRSGTLYYPMWLAYAAAHV
                     RRLGHTPLLIDAPASRLSESETLDRLNQFAPQVVLVETATPSIVNDLHFASRVKVNGN
                     EKLVYVCGTHASALPGDCLREAPEIDGVIYGEYEVPLEEIVRCLQQGRRVPIGEGICS
                     RGGEQKRERLRFLQNMDDGPFVSEIYKEFLPIDRYFNPNAHYPMVALVSGRGCPYGCS
                     FCVFPQTLMGRGYRQRSVDSLIAEFRWISHHLPHVKGVFLEDDTFTTNKPRVLDFCEK
                     LQRENLPLTWTANARADVDEETLRAMKNAKVRGLCVGFESGSQVILDAMGKALTLEKM
                     EAFAVAAQRAGVKVHGCFVVGFPQETPVTLQQTLQFALRLPLDTAQFYPLMVYPGTRA
                     YAEAARTGMIAAPTFRDWLSPTGQHQCVVRNSRFTPQQLVAFCNFARRRFYLRKSWLW
                     RNFRLILTDADERARVLRAAATFSRHLLEDR"
     gene            1695..2672
                     /locus_tag="PKO06_08745"
     CDS             1695..2672
                     /locus_tag="PKO06_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69771.1"
                     /translation="MMTRVPAASVVIPVLDSREKLTRCLEALRCQKTTLAYEVIVVND
                     GSTEDLSAVRSAMGSRLPLVWLDLPGNSGPAVARNAGIEAARGDIILFTDADCRPEPD
                     WLDQMVQPFRDPRVTGAKGVYLTDQRDAWARLAQTEFEERYELLNTCQDIDFVDTYSG
                     AYRRGDLVAVKGFDTAFPRADNEDVDLAFRIKKRRGRFAFVPEAKVWHHHREGMWPYI
                     KLKFWRGYWRMQVYRRHPEKAGRESYTPLTLKAQLLLLVVFPVTLFLTTRCRRWFYAW
                     MVGWFTSCLPLMRRASSAGLGLTSRVPVFAAARGLALLAGMLWGMVRKA"
     gene            2838..>3705
                     /locus_tag="PKO06_08750"
     CDS             2838..>3705
                     /locus_tag="PKO06_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69772.1"
                     /translation="MQKYFVENLDQKFEYKSYRIFFAVRDSLPAEEVLNTLDRASSGL
                     LTIFDLAETPKLDVFIYSDPASIEKVTGKPFQQGEGLRVVPSDNAMLLCSCRLPNMID
                     EEVSRQLSYLIFNEEMGDREIAPHQYRCPSWLREGACLQVASRLRRDTKPYLLGGWAS
                     LQEAEKADRLIKPNILEKSVYLIPDPQRRALPDRAASGKDRLGAGRRARHQDRHRQDA
                     HGPRDTLPASRVAALSRRGSRGVADSPACRASGPRSRPARRVHSAGRGGVWRARTGRG
                     PPPGGPSAPTAGR"
BASE COUNT          752 a         1027 c         1088 g          838 t
ORIGIN      
        1 gatagcgcaa atcttccctg gacgagaagg gaaaaaatgg gggaacgcgg aatttgccgt
       61 cacagcgaca tgttcggact ggcttcagca gaggaaagcc acgtcgattc caggtgcaac
      121 catgtggttt acctgattgc ccgcaggctc gacgcttgat gccacgtagc gtattctgag
      181 gtatagtatg ggcagcctga ccgcccgggt gaagaatctg cgctggcgat cttcaccgtt
      241 aggagtcgaa ccatgacctt acgcgttgcg ttggtgaatc caccgtttct gccggattat
      301 tcccgtgggc agcgatcgcc ggccgtcacc cgcagtggca cgttgtacta tcccatgtgg
      361 ctggcctatg ccgcggcaca tgtgcgtcga ttggggcaca caccgctctt aatcgatgct
      421 ccagcgtctc gcctttccga atcagaaact ctggaccgtc tcaatcagtt cgctcctcag
      481 gttgttctgg ttgagacagc gacaccgagt atcgtgaatg atctgcactt tgcctcgcgc
      541 gtcaaggtga acggcaacga aaaactcgtc tatgtgtgtg gtacccatgc ctcggcttta
      601 cccggcgact gtctgcgtga agctcccgag atagatggtg ttatttacgg ggaatacgaa
      661 gtgcctcttg aagagattgt tcgctgcctt caacagggtc ggcgggttcc cattggcgag
      721 ggcatctgca gccgcggggg agaacagaag agagagcgat tgcgctttct gcagaacatg
      781 gatgatggcc catttgtttc agaaatctac aaagagtttc tccccatcga caggtatttc
      841 aatccgaatg cgcattaccc gatggttgcc ctggtttcag ggcgaggctg tccctacggt
      901 tgttcgttct gtgtctttcc acaaaccctt atgggaaggg gatatcgaca acgatccgtt
      961 gattcgctca tcgcggagtt tcgatggatc tcacaccacc tgccacatgt gaagggcgtc
     1021 tttctcgaag acgacacctt caccaccaac aaaccgcgcg tgctcgattt ttgcgaaaaa
     1081 cttcaacgcg aaaatctgcc cctgacctgg acggcaaacg cccgcgcgga tgttgacgaa
     1141 gaaaccctgc gtgccatgaa gaatgcaaag gtgcggggcc tgtgtgttgg cttcgagtct
     1201 ggaagtcagg tcattctcga tgcaatggga aaagctttga cgttggagaa aatggaggca
     1261 ttcgctgttg cggcgcagcg ggctggcgtg aaggtgcatg gctgtttcgt ggtcgggttt
     1321 ccgcaggaaa ccccagtgac attgcagcaa accctgcagt tcgccttgcg cctgcctctt
     1381 gataccgcac agttctatcc cttgatggtc tatccgggca cccgagccta tgccgaagcc
     1441 gcaagaacgg gcatgattgc cgctccaacg tttcgggact ggttgtcgcc gacaggacag
     1501 catcagtgtg tcgtgcgcaa ctcgcgtttc acaccacaac aactggttgc cttctgtaac
     1561 ttcgcccgcc ggcgattcta tctgcgcaaa tcgtggctgt ggcgcaactt ccgactcatt
     1621 ctgacggacg ctgatgagcg agctcgagtt ctgcgcgccg cggcgacgtt ttcccgacat
     1681 ttactggaag accgatgatg acccgcgttc cggccgcatc agttgtcatt ccggtacttg
     1741 attcccggga aaaactgaca cgctgtcttg aagcgctgcg ttgtcagaag acgaccctgg
     1801 cctacgaagt catcgtggtg aatgacggct cgactgaaga tttgagtgcg gtgcgatcag
     1861 cgatggggtc gcgattacct ctggtttggc tcgatctgcc aggaaactct ggcccggcag
     1921 tggcccgcaa tgcaggcatt gaggccgcgc gcggagacat tatcctcttc accgacgccg
     1981 actgccgccc cgaacccgat tggctcgatc agatggtgca gcctttccgc gatccgcgtg
     2041 tgacgggtgc caaaggggtg tatctcaccg atcagcggga tgcgtgggcc agactggccc
     2101 agacggagtt cgaggaacgc tatgagctct tgaatacctg ccaggacatc gattttgtcg
     2161 atacttactc cggagcctac cgccgaggtg atttagtggc tgtgaagggc ttcgataccg
     2221 cttttccgcg agcggataat gaggacgtcg accttgcgtt ccgtatcaag aaacgtcggg
     2281 ggcgttttgc gtttgtccca gaagcaaagg tgtggcatca tcaccgtgag ggaatgtggc
     2341 catatataaa actgaaattc tggcgtggct actggcggat gcaggtctat cgacgtcacc
     2401 cggagaaagc cggtcgcgaa agctacaccc cgctgacact caaggcacag ctgcttctgt
     2461 tggtcgtgtt tccggtgacc ctgtttctga cgacacgctg tcgtcggtgg ttctatgcct
     2521 ggatggtcgg gtggttcacc agctgtctgc cactcatgag acgcgcttcc agcgcaggtc
     2581 tagggttaac gtcgcgtgtg ccggtgttcg ccgcagcccg tggactggcc ctgctcgccg
     2641 gcatgttgtg gggaatggtg cgcaaggctt gagcgtcaga gtgcataccg tgtccagatt
     2701 gccacacact gactgtcgcc cggtgtacca gaccagaaga gacgctcgtt gcgatctggg
     2761 ctgagtcgac cgctcgaaca gaccgcttgc ccggatccga gggtctatga tacagtttgt
     2821 gacggaagag ggatctcatg caaaaatatt tcgttgaaaa ccttgatcag aaattcgagt
     2881 acaagtcgta ccgcatcttt tttgcggttc gtgacagctt gcctgctgaa gaagttctga
     2941 atacgcttga ccgagcatcg tcagggcttc tgacgatttt cgatctggca gaaactccga
     3001 agctcgacgt cttcatctat tctgaccccg cttcgatcga gaaagtcact ggcaagccct
     3061 tccagcaggg tgaaggattg cgcgtggtgc cgtccgacaa tgccatgctt ctctgttcgt
     3121 gtcgattgcc gaacatgatc gacgaagaag tgtctcggca gttgagctac ctgattttca
     3181 atgaggaaat gggcgatcgc gagattgctc ctcaccagta tcgctgtcca tcgtggttac
     3241 gcgaaggcgc ctgcctgcag gtcgcatcgc gcctgcgtcg tgacacgaaa ccgtatctgc
     3301 ttggtggttg ggcttcgttg caggaagccg aaaaagcgga tcgcctgatc aagccgaata
     3361 ttctcgaaaa aagcgtgtat ttaatccccg atccacagcg tcgggctctg cccgaccggg
     3421 ccgccagcgg gaaagatcga ctgggcgccg gccgtcgcgc tcgacaccaa gatcgccatc
     3481 gtcaggatgc gcatggaccg agagacaccc tccccgcttc gcgggttgcg gcgctcagcc
     3541 ggcgaggatc tcgaggagta gctgattcgc cagcctgccg cgcatcaggc ccgcgatctc
     3601 gacccgcacg ccgagtgcat agcgcgggtc gcggaggtgt ttggcgagcc agaacgggtc
     3661 gcggtcctcc accaggtgga ccatccgctc caaccgctgg gcgtc
//
LOCUS       DAORHD010000359         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27493_length_3701_cov_3.285793, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000359 DAORHD010000000
VERSION     DAORHD010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27493_length_3701_cov_3.285793"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKO06_08755"
     CDS             <1..620
                     /locus_tag="PKO06_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69773.1"
                     /translation="SFTYRKDFICHHATVLQTALQNHGIDMNDLLDRCMYPHHRYHGR
                     DGIIYVGPVHVSGMSWKYFLDQVKRFRSQRLARWFPKVEGKGTIVSNGRPLAEVLRDI
                     AWKSGVPIRWDQPVAMPLYCSFKERPWHQILENIILYNGLILAPAQDALVVSGPWPQN
                     QSQAAAPAGAVSTPVTAPAPAPTPAPASPSAFVLPPAPPLPAAGQ"
     gene            645..1520
                     /locus_tag="PKO06_08760"
     CDS             645..1520
                     /locus_tag="PKO06_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69774.1"
                     /translation="MRCLSFRSPASIVFLLAVCLSVVTLGAATKPAVQPLEMYEQWSG
                     FNSGFSGQPAYQFIRDYETFKSFWARHKPDEFMPQIDLDKLQVFVWCPGPVLSGERLV
                     EALRFYRSAEGLTLVVRTELHRDSGAWQAPFLIAFLQKTSGHVRIMRPGSKARREPPL
                     MPLFHWQDMGLPAIYHPAAVSLQAPKRPEKAWRNPWELDIPNPIARPAWTKQPTATAV
                     EPAVPVTETAEPKPAASASAAVSGTKPAGQTGTKSTSSTATTGTTTTTSSNDPTDIGN
                     ASGADPLQDPFNLDF"
     gene            1528..2049
                     /locus_tag="PKO06_08765"
     CDS             1528..2049
                     /locus_tag="PKO06_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNV69775.1"
                     /translation="MIQAYIGEGKGKTTAALGLIMRATGAGWKVKLIQFDKGGDAYAY
                     HERHSLQRLGVEVVVTGCNRQLPDGGFRFRTNEDDLAEARRGLQEAKHALVLNYRLLV
                     LDELLTAVTYGLIPAADLHELLRSVPSELECVVTGRVNDLALVEACHLVSRIEKVRHY
                     FDAGQPARPGIEF"
     gene            2166..3047
                     /locus_tag="PKO06_08770"
     CDS             2166..3047
                     /locus_tag="PKO06_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV69776.1"
                     /translation="MTSDLNGTDWFLYALISTIIYGVFEFTYKLAARGGYSSIQIVNK
                     AAITVTVLSGILILHNQSHFTPLPWILLYGFLNATCFASGTIAQISALRYMPTSHVFP
                     IAKMSSIICIFLGFAFLGDRPTVWQWAGILMALGVILLVGYDMKSGGGTEQATDPARG
                     LKLAIGAAFGVAFSMFVGKLASMHVPALNYIFSSYGFSAIYTYLIERRFGNAKRRLDA
                     RGLGFGVFIGTMNFAGYYLLLRAFSLGPLALVQGIFSTTMVITIPLSVLILKERFSLI
                     KALCVVLALGALILIRL"
     gene            complement(3112..>3701)
                     /locus_tag="PKO06_08775"
     CDS             complement(3112..>3701)
                     /locus_tag="PKO06_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV69777.1"
                     /translation="GVFMPEFCSTVCDPETSQGGKPNVHYTVGAAAVVLEIDKDTGKM
                     DVRKIALAADVGKAINPDMVRGQIVGGMLQGLATALYEEIVIDEHGKLLNPNFTDYKI
                     PTARDIPMEVVPIIVETPQADGPFGARGIGEHTMIASAPIIGNAIYNALGIRMTAMPM
                     TAERVALSKHLKLEKRVEALYSKRSESYCKRGTKP"
BASE COUNT          661 a         1149 c         1093 g          798 t
ORIGIN      
        1 tctcgttcac gtaccgcaag gatttcatct gccatcatgc gacggtgctg cagacggcgc
       61 tgcagaatca cggaatcgat atgaacgatc tgctcgaccg ctgcatgtat ccgcatcacc
      121 ggtatcacgg ccgcgacggg atcatctatg tcggaccggt gcatgtcagc ggcatgagct
      181 ggaaatactt tctcgatcag gtcaagcggt ttcgcagtca gcgcctcgca cgctggtttc
      241 ccaaggtcga aggaaagggc accatcgtca gtaacggtcg tccgctcgcc gaggtgctgc
      301 gcgacatcgc ctggaagtcc ggcgtcccca tccgctggga ccagcccgtc gccatgccac
      361 tctattgttc ctttaaagaa cggccctggc accagattct cgaaaatatc atcttgtaca
      421 acggtctgat tctggccccc gcgcaggatg cgctggtcgt gagcgggccc tggccgcaga
      481 accagtctca ggctgcggca cctgcgggcg ctgtctcgac gcctgtcacg gctccagccc
      541 ctgctccgac tcctgcgccc gcctcgcctt cggcgtttgt cctgccaccg gcgcccccgc
      601 tgcccgccgc cggccagtag agccttacgc aaggagccct gcccgtgcgc tgcctgagtt
      661 ttcgttcgcc cgcctcgatc gtgttcctgc tggccgtgtg tctttcggtc gtgacgctcg
      721 gcgctgccac gaaacctgcg gtgcagccgc tcgagatgta tgaacagtgg tcgggcttca
      781 acagcgggtt ttcggggcaa ccggcgtacc agttcattcg cgactacgag accttcaagt
      841 cgttctgggc caggcacaag cctgacgagt tcatgccgca gatcgatctc gataaactgc
      901 aggtgttcgt ctggtgtccg ggcccggtgc tgagcggtga gcgactggtc gaagcgctgc
      961 ggttttaccg ctcagcggag gggctgaccc tcgtcgtccg aaccgagctt caccgtgatt
     1021 caggcgcgtg gcaagcccct tttctgatcg cctttctcca gaaaacctcg ggacacgtgc
     1081 gcatcatgcg ccccggaagc aaggcacgac gcgagcctcc gctgatgccg ttattccact
     1141 ggcaggatat ggggctgccg gcgatctatc acccggcggc ggtgtcgctg caagcaccga
     1201 agcggccaga gaaagcctgg cgcaacccct gggagctcga cattcccaac ccgattgccc
     1261 gccccgcctg gaccaaacag cccaccgcga ccgcggtcga acctgccgtt ccggtgaccg
     1321 aaacggccga gccaaagccg gccgccagtg ccagcgccgc ggtgtcggga acaaagccgg
     1381 ctggccaaac tggcacgaaa tcgacgtcgt cgactgcgac aactggtaca accacgacga
     1441 ccagctcaaa tgatccgacc gacatcggga acgcttccgg tgccgacccg ctgcaggatc
     1501 ccttcaacct ggacttctga gggaagcatg attcaagcct acatcggcga gggcaaaggc
     1561 aagaccacgg ccgcgctcgg gctcatcatg cgggccaccg gagcgggctg gaaggtgaag
     1621 ctgatccagt tcgacaaggg tggtgatgcc tatgcctacc acgaacgcca cagcctgcaa
     1681 cgtctcggag tcgaggtcgt cgtgaccggt tgcaatcgcc agctgcctga cggcggtttt
     1741 cgctttcgca cgaacgaaga cgacctggcc gaagcccgtc gcgggttgca ggaagcgaaa
     1801 cacgcactcg tactgaacta tcgcctgctg gtcctcgacg agctgctgac ggccgtgacc
     1861 tacgggttga ttcccgccgc cgatctgcac gagttgctgc gttccgtgcc cagtgagctc
     1921 gaatgtgtgg tgaccggtcg agtgaacgat ctggcgctgg tcgaagcttg tcatcttgtc
     1981 tcgcgcatcg agaaggtacg ccattacttc gacgccggcc aaccggcgcg accgggcatc
     2041 gagttctgac tttcgcccga attgtgtgtc tgagattgaa atgataagat agcgctgatc
     2101 tgtatcaatg ttgacgagag gatgatgctg aatgggtcac gccgttcggt atcgtaacca
     2161 agcccatgac ctcggatctg aacggaaccg actggtttct ctacgccctg atttcgacga
     2221 tcatctacgg ggtttttgag tttacctaca aactggccgc gcgcggcgga tacagcagta
     2281 ttcagatcgt gaacaaggcg gcgatcacgg tcaccgtcct gtccggaatt ctgattctgc
     2341 acaaccagag ccacttcacg cctctgccct ggatcctcct ctacgggttt ctgaacgcca
     2401 cctgttttgc cagcggaacg atcgcgcaga tctcggcctt gcgctacatg ccgacctcgc
     2461 atgtgtttcc cattgccaag atgagttcga tcatctgcat ctttctgggg tttgcctttc
     2521 tgggcgatcg tccgaccgtc tggcagtggg caggcattct gatggctctg ggcgtgattc
     2581 tgctcgtggg ctacgacatg aagtcggggg gcggtaccga acaggcaact gatccggctc
     2641 gcgggctcaa gttggcgatc ggtgcggcct tcggcgtcgc cttctcgatg ttcgtcggaa
     2701 agcttgcttc catgcatgtg cccgcgctga actacatctt ttcctcgtac ggtttttcgg
     2761 ccatctatac gtatctcatc gaacgtcggt tcgggaacgc gaaacgtcgt ctggatgcgc
     2821 gtgggctcgg gttcggggtc ttcatcggca cgatgaactt cgccgggtat tacctcctgc
     2881 tgcgcgcgtt cagccttggc ccgttggcgc tcgtgcaggg catcttctcc acgacgatgg
     2941 tcatcaccat cccgttgtcc gtcctgattc tcaaagaacg cttctcgctg atcaaggcac
     3001 tttgcgtggt gctcgcgctg ggggcgttga ttctgattcg attgtaaccc gcatgaaaaa
     3061 aggaaggcgc ccgtaggcgc cttcccgttc agacggttcg tcagtggtcg atcagggttt
     3121 ggttccccgt ttacagtagg attcggaccg ctttgagtac aaggcctcga ctcgcttttc
     3181 gagcttgaga tgcttgctca gggcgacgcg ttccgcggtc atcggcatgg cggtcatgcg
     3241 aatgcccagc gcgttgtaga tcgcgttgcc gatgatcggt gccgaggcga tcatggtgtg
     3301 ttccccgatg ccgcgggctc cgaacggacc atccgcctgc ggggtttcga cgatgatcgg
     3361 aacgacctcc atcggaatgt cgcgtgccgt cgggatcttg tagtccgtga aattcgggtt
     3421 cagcagtttg ccatgctcgt caatgacgat ctcttcatac agcgctgtcg ccagaccctg
     3481 aagcatgccg ccgacgatct gcccgcgcac catgtccggg ttgatggctt tgccgacatc
     3541 ggctgccaga gcgatcttgc gcacgtccat tttgccggta tctttgtcga tctcgagcac
     3601 caccgcggcg gcaccgaccg tataatgcac gttcggtttg ccaccctggc tggtttccgg
     3661 gtcgcagacc gtcgaacaga actccggcat gaagaccccg g
//
LOCUS       DAORHD010000360         3693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27563_length_3693_cov_3.581089, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000360 DAORHD010000000
VERSION     DAORHD010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27563_length_3693_cov_3.581089"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1516
                     /locus_tag="PKO06_08780"
     CDS             <1..1516
                     /locus_tag="PKO06_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69778.1"
                     /translation="AFLAASVAWGREFIEPYTQTQLRSAGFSNALISRAARTPGQEYT
                     RSVDARTVRGQGDSLPPISEGGDGAAPAGTDAPATPAPTDSGGTAFPDPLGAPPAPAA
                     AGGSDASTGAGSTAPAPVVAEPAKPPEKEEVKSSKSSSSSSKSSSTKKSGAKKKKKKK
                     SSAPVVSNGAIAVTQAPLLPSPTPYTVGRALPAPPPLAMGSGQEIYSQDPVQLPPVSA
                     LPEPSRPTLPPVTRDPIMMTPIGEAVSRVLACNFSGLTGLSMTTSADISREGSFKMGF
                     HTNWFTLDRVYDRPLASNESGDLFEAPIFFNYAVTNDLETAIMLPILNYTVKSRILWS
                     RDFRESGVGDTKLSFKYRIFDNGQYQMRGAFGMGFKFPTGSDAKGLGTGKTDFETFCA
                     FSKNFERIIAHLNLGYVMTGDPNTQFYPDGLADIFYYNIGLEYPHNNNVTIATEIAGQ
                     DWGAEGLKLDVIPTLRYTPTENFCFEVAVPVAVTNDQRYGYNYRVTFGVTSFFR"
     gene            1631..2611
                     /locus_tag="PKO06_08785"
     CDS             1631..2611
                     /locus_tag="PKO06_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69779.1"
                     /translation="MENDRKLGVLGALGFLSFWMVCGYGFLAAMGYGFLSDINYVDPR
                     MPLAWYGAAFERDYSELAPIYRKLETQYHRTRRSLGFLEIAEMDGSLVRVPGGGVRYT
                     VASLGFSLDSDGMKRWYEQTPSMKKAWERWKRDATPIIVIPDGYGFPYVPNADEKTLA
                     RWFPSYPETFRLRALFDGRRTEPPTLVEQVGECGWDGSQFSFPLVWRMNEFGAKREQK
                     KAGLHYFLIVAIIGGIATLMLMRWPMPDVGEIEILRSFAIGFGLFSLLSLPTYRTCYS
                     SRDVTDDEVIEPRVLEMIRKDCRAYLDDLQRESKISNDDKQMMLKLLNRR"
     gene            complement(2620..>3693)
                     /locus_tag="PKO06_08790"
     CDS             complement(2620..>3693)
                     /locus_tag="PKO06_08790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69780.1"
                     /translation="TFFMRVQERFFPITPVTIEVVRHCQQACRYCYRLPASTGTPEFL
                     APETLRICLERLKSQFGRMAITISGGEPTLHTQLPELVRLCREYSRAVTLISNLGCMT
                     FELASELARSNLQHVQTTFISPIPAEHDALCGTGTFSRWLTGFEAARRAGLNLGAILL
                     VTPATLERIPVAIDFLLGLGVKGWMVNRFNAGYRTTADASSLFLDQTQLERMLSLLEG
                     TAARRGLTIPFGVPIPVCLADPKRYPHLVFASCPLGRKGQYWTVGADGFLRPCNHLPI
                     KLGDLRSHSIAEMLQQTTWQQILAHLHTPPPSCSGCAFHNECQGGCRAAAHTWNGSLA
                     HCDPWVEQLLSEKHAHLENPRGT"
BASE COUNT          798 a          986 c         1113 g          796 t
ORIGIN      
        1 ggcatttctc gcggccagcg tggcctgggg ccgagaattc atcgagccct atacgcagac
       61 acagcttcgc tcagcaggtt tcagcaatgc gcttatttcc cgggctgcca gaacacccgg
      121 acaggaatac acgcggagtg tcgatgcacg aaccgtgcgt ggacagggtg acagtctgcc
      181 gccgatctcc gagggtggag atggcgcggc acccgcgggc accgatgctc ccgcaactcc
      241 cgcccctacc gattccggcg gcacggcgtt cccggatcca ctgggagctc cacccgctcc
      301 cgcggctgcg ggtggaagtg atgcatccac gggggcgggt tcgaccgcgc ctgcgcccgt
      361 cgttgctgaa ccggcgaagc cacccgagaa ggaagaggtg aaaagttcga aatcaagctc
      421 ttcttcgtcg aaatcgtcta gtacaaaaaa gtctggcgcg aaaaagaaga aaaagaagaa
      481 atcttccgcc ccggtagtca gcaatggggc tattgcggtg acccaagctc ctctgttgcc
      541 ttcgccgaca ccgtatacgg tgggtcgcgc gctgcctgct ccgcctccgt tggccatggg
      601 aagcggccag gaaatctact cgcaggatcc cgtgcaactg ccgccggtca gtgcgctgcc
      661 tgagccttcc aggcccactt taccaccggt gacccgagat cccatcatga tgacgccgat
      721 tggcgaagcg gtttctcgcg tgcttgcctg caacttctca ggtttgaccg gcctgagtat
      781 gacgacctcg gctgatatca gccgcgaagg gtcgttcaag atgggctttc ataccaactg
      841 gttcacgctt gatcgagtct acgaccggcc cctggcaagc aacgaaagcg gtgatctgtt
      901 cgaagctccg atctttttca actacgccgt caccaacgat ctggaaacgg cgatcatgct
      961 gccgatcctc aactacactg tgaagtcacg cattctgtgg tcgcgtgatt ttcgtgagtc
     1021 cggggtgggt gacacgaaac tctcgttcaa ataccgcatc ttcgacaatg gtcagtatca
     1081 gatgcgcggc gcattcggta tgggcttcaa gtttcccacc gggagtgacg ccaaagggct
     1141 gggaacgggc aagaccgact tcgagacctt ctgtgcgttc agtaaaaact ttgagcgcat
     1201 catcgctcac ctgaatctgg gctatgtgat gactggtgat ccgaatacgc aattctaccc
     1261 ggatgggctg gcggatatct tctattacaa catcggtctc gagtatccgc acaacaacaa
     1321 cgtgacaatc gcgacggaaa ttgccggtca ggattggggt gccgaagggc tgaagctcga
     1381 tgtgattccg actctacgct atacgccgac cgaaaacttc tgtttcgagg tcgcggtgcc
     1441 cgtggctgtg accaacgacc agcgctatgg atataactac cgtgtgacct ttggagtgac
     1501 ttcgttcttc cgctgaactg actcgtacaa atcacgaacg cccagccgtt gtgctgggcg
     1561 ttcgtcttta cgatgacttg catcaggatt tcgtaaccgg gtattctgtg ggcaacggga
     1621 gagcatgatc atggaaaacg atcgcaagct tggagtgctc ggagcactcg gttttctgag
     1681 cttttggatg gtgtgcggct acggctttct cgccgcgatg ggctacggct ttctttctga
     1741 tataaactac gttgacccgc gaatgccgct ggcatggtat ggggctgcct tcgaacgcga
     1801 ctattctgag ctggccccta tctatcgaaa acttgagact cagtatcaca gaacgcggag
     1861 gtcgctcggg tttctggaga ttgctgagat ggacggttcc ttggtacggg ttccgggagg
     1921 gggggtgcgg tacacggtcg cgtcgctcgg cttctccctc gactctgatg gcatgaaaag
     1981 atggtatgaa caaacaccat ccatgaaaaa ggcgtgggaa cgttggaaac gagatgccac
     2041 accgataatc gtgattcctg atggttatgg ttttccctat gtcccgaatg cggacgaaaa
     2101 gaccctggcg cgttggttcc cttcataccc tgagactttt cggcttcgcg cactctttga
     2161 tggccgacgc accgagcctc cgaccctggt cgaacaggtt ggcgaatgtg gctgggatgg
     2221 atcacagttc agctttcccc tcgtctggag aatgaacgaa ttcggggcca agcgtgaaca
     2281 gaagaaagct ggcttgcact attttctcat cgtggcgatc attggcggca tcgccaccct
     2341 gatgctgatg cgttggccta tgccggatgt cggggagatt gaaatcctga gaagctttgc
     2401 catcggcttt ggtctgtttt ctctcctgtc gctgccaacc tatcgcacct gctactcgag
     2461 tcgcgatgtg acagatgacg aagtcatcga accgagagtg cttgagatga ttcgcaaaga
     2521 ttgccgagcc tatctcgatg atctgcagcg agaaagcaaa atcagcaacg acgacaaaca
     2581 gatgatgttg aagctgctga accgtcgctg atcgaaacct cacgtccctc gcgggttttc
     2641 gagatgcgcg tgcttttctg acaggagctg ctcaacccaa ggatcacagt gcgcgagaga
     2701 gccattccaa gtgtgagccg cggcgcgaca gcctccctga cactcgttat ggaacgcgca
     2761 cccagaacag gagggtggtg gtgtgtgcag gtgcgcgaga atctgttgcc aggtcgtctg
     2821 ctgaagcatt tcagcaatgc tgtgcgagcg caggtcgccc agtttgatcg gcagatggtt
     2881 acagggccgc agaaatccgt cggctccgac ggtccaatat tgccctttgc gtccgagtgg
     2941 gcaggatgca aaaaccagat ggggataccg tttggggtcg gcaagacaga cgggtatggg
     3001 gacgccgaag ggaatggtca ggccccgtcg tgcggcggtt ccctcgagca gcgagagcat
     3061 gcgctcaagt tgtgtttgat cgagaaacag ggaagatgca tccgcggtgg tgcgatatcc
     3121 cgcattgaaa cgattgacca tccagccttt gacgccgagg ccgagcagaa agtcgatggc
     3181 gacgggaatt ctttcgagcg ttgcgggcgt gaccagcagg atcgcaccga gattcaatcc
     3241 ggcacgacgt gcggcttcaa atccggtgag ccatcgcgaa aaagtgcctg tgccgcacag
     3301 agcgtcgtgt tcggcgggga tcggggagat gaaggtggtt tgtacgtgtt gaaggtttga
     3361 gcgagcgagt tcagaagcca gttcaaaggt catgcagcca agattgctga tgagggtgac
     3421 agctcgggaa tactcacgac acaagcgaac cagttccggc agctgtgtgt gaagggtggg
     3481 ttcgccgccg ctgatggtga tcgccatgcg tccgaactgg gatttcagtc gttccaggca
     3541 gattcgcagt gtttcggggg cgagaaactc cggggttcct gtcgaggccg gaagacggta
     3601 gcagtaacga caagcctgct gacagtggcg aacgacctcg atagtcacgg gagtgatcgg
     3661 gaagaaacgc tcctgcacgc gcatgaaaaa ggt
//
LOCUS       DAORHD010000361         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27615_length_3688_cov_4.252133, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000361 DAORHD010000000
VERSION     DAORHD010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27615_length_3688_cov_4.252133"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1781)
                     /locus_tag="PKO06_08795"
     CDS             complement(<1..1781)
                     /locus_tag="PKO06_08795"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69781.1"
                     /translation="MIFGRSEPRRGATRLQTTAFVLLALCCGAFGIRFPGNPFSHFIE
                     RAELSSYDLRVRSQGGVPFDAERLLVVGIDDSCFQHCAGNERTKSDAKWPFPNAWHAE
                     VARKLLNWGAAAVAFDILSFANPSRTLYEFEDRAFADLATQSGRIVLSAKFLHAGADS
                     WHGYEMRQHAGQQQVIRETWTLPHTTFKAFPTGYVNFPLDQDGAIRRFLPSYKRLSLF
                     PIKPLAHEALRQAVLLPASVPAELVGSTPTCLAFSDPREIASGSLFYHQVLLASETGE
                     SVAALVARVKGRVVFVGPTFTEAHDFFFTPFTGRRESDLPTPGVYLHAMVANTLLKQL
                     AIRRSPLHHERLLAAFAVLVGLAASLLLQPLSGLIVFCVLMLGFVIIGYAVFNTLFIW
                     LPLASPILAALGGFVPLLGLRLIVSESRRALINKMFSAYVSKEVLHFVQDNPERLRLE
                     GERREVSVFFSDLRGFTTISEGVSPEQLSEVMNTYLTPMTETIMAERGYLDKYIGDAI
                     MAVFGAPMPDEQHALHACRAAILQRRRLFEVAKWIESHCGVQVAARMGINTGVVSAGN
                     MGSQDRFQYTVMGDVVNQASRFEGANK"
     gene            complement(1778..2467)
                     /locus_tag="PKO06_08800"
     CDS             complement(1778..2467)
                     /locus_tag="PKO06_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69782.1"
                     /translation="MSIVFRNGLLCLLLILASTSSMLAETPGATGFQIVSCEGDVLHV
                     PDGTEAPEPAKPGVTITSGRFITLESGKAELKFPSGKTFAIPANTLLMIDPKQETLEN
                     ACAAAAAIRDRSVLFVLPREGETITAGKPVALLFGVQIDALDLASTSGLKVLALDAAS
                     ADKPKEIELATLALGDNPSLGAGTMGYRYFRLESARGLPEGEYEIIVKTQATPAGKKV
                     GKARAISVTKP"
     gene            2814..>3688
                     /locus_tag="PKO06_08805"
     CDS             2814..>3688
                     /locus_tag="PKO06_08805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNV69783.1"
                     /translation="MNVSPRKVCWLSLLVFLAISLACRAQAVSPVAAASETHLVPGAS
                     TTTMPVFDAASRSLETKIDSDIAIIAPVASEASRSWFIAAVGDIMVHETQLKTHWDAG
                     AKQYLFDDDFIHIRPLLASADLMIGNFETTLGGPGRKYSGYPVFNTPDTLAEALKRAS
                     FDVLMTSNNHCLDTGFAGLKRTHAVLSGLGFGVIGTKNDEKAESFLVRELNGLKVGLA
                     AYTYETSRKQGKATLNGGLMPPGASTLINSFATETLEANLASMSERIRILRSRGAELI
                     VFVLHWGVEYRPQPTP"
BASE COUNT          820 a         1013 c         1093 g          762 t
ORIGIN      
        1 atcttgtttg ccccctcaaa gcgtgaagcc tggttgacca catcacccat gaccgtatac
       61 tgaaaccggt cctgcgagcc catgttgccg gcgctgacca cgccggtatt gatacccatg
      121 cgggcggcaa cctggacgcc gcagtgagac tcgatccact tggcgacctc gaagagacgc
      181 cgccgttgca gaatagcggc ccggcaggca tgaagggcat gttgctcgtc gggcatcgga
      241 gcgccgaaca cggccatgat tgcatcgccg atgtacttgt cgagataccc gcgctcggcc
      301 atgattgtct cggtcatggg cgtgagatac gtgttcatga cctcagaaag ctgctcgggc
      361 gagactccct cagaaatcgt cgtgaatccg cgcaggtcag agaaaaaaac agatacctct
      421 cgacgttcgc cctcgagcct gagacgctct ggattgtcct ggacaaaatg cagaacttct
      481 ttggaaacat aggccgagaa catcttgttg atcagagctc gtcgactttc ggagacaatc
      541 agtcgaagtc ccagtagagg cacgaatccg ccaagggctg ccagaatcgg cgaggcaagt
      601 ggcagccaga taaacagggt gttgaagacg gcatacccaa tgatgacaaa tccgagcatc
      661 aagacgcaga aaacaatcag gccgctgagg ggctgtagaa gcagagatgc tgccaagcca
      721 accaggacgg caaaggcagc taatagacgt tcgtgatgaa gcggtgaacg ccgaatcgcc
      781 agttgcttga gcagggtgtt ggcgaccatg gcatgcaaat acacaccggg agtaggcaga
      841 tctgattcgc ggcgtccggt aaacggagta aaaaagaagt cgtgagcctc ggtaaaggtc
      901 ggaccgacaa agacgactct gcctttcacg cgtgcaacca gcgcggcgac cgactcgccg
      961 gtctcgctgg ccaggagcac ttgatgatag aacagcgaac ccgaggcgat ctcgcgtgga
     1021 tcggaaaacg cgagacaggt tggggttgag cccaccagtt cggccggaac cgaagccggc
     1081 agaagaaccg cctggcgcaa ggcttcatgg gccagcggct tgatcggaaa gagactcagg
     1141 cgtttatagg agggcaggaa ccttctgatg gcaccgtcct ggtcgagagg aaagttgaca
     1201 tacccggtcg ggaaagcttt gaacgttgtg tgcggcagtg tccaggtttc gcgtatcacc
     1261 tgctgctgtc cggcatgttg gcgcatttca tacccgtgcc agctgtcggc cccggcatga
     1321 aggaacttgg ctgatagaac aatgcgcccc gattgcgtgg cgagatcggc aaaagcacga
     1381 tcttcaaact cgtacaaagt tcgactggga ttcgcaaaac tgagaatgtc gaaggctacc
     1441 gctgctgcac cccagttgag cagtttgcgc gcgacttccg cgtgccaggc gttcggaaac
     1501 ggccacttgg catcgctctt cgttcgctca tttccggcac aatgctgaaa acaggaatcg
     1561 tcgatcccga cgaccagaag ccgctcagca tcaaagggga cccctccctg agaccggacc
     1621 cgcaggtcgt aactcgataa ctcggctctc tcgatgaagt gtgaaaacgg gttccccgga
     1681 aaccggattc cgaaagcgcc acagcacaac gccaacagaa cgaaggcggt ggtctgcagg
     1741 cgggtagcgc cccttctcgg ttccgagcgt ccgaaaatca cggtttcgtt acgctgatgg
     1801 cgcgagcttt gccaactttc ttccctgcgg gcgtggcctg agtcttgacg atgatctcat
     1861 actcgccttc gggtaaaccg cgggcagact cgagtcgaaa gtagcgatat cccatggtac
     1921 cagctccgag cgacggattg tcgccaagag cgagagttgc cagttcgatt tctttcggtt
     1981 tgtctgcgct cgcggcgtcg agagcgagaa ctttcagtcc tgaggtcgag gccagatcga
     2041 gggcgtcgat ctgcacgccg aacagcaggg caaccggctt gccggcagtg atggtctctc
     2101 cttcgcgcgg cagaacgaac agcacactgc gatcacgaat ggcggcagcc gcggcgcagg
     2161 cattctcgag ggtctcttgt ttcggatcga tcatcagcag tgtgttggcg ggaatcgcga
     2221 atgtcttgcc tgaaggaaac ttcagttccg cttttccact ttcgagagtg atgaaacgcc
     2281 cactggtgat ggtgactccg ggtttcgccg gctccggagc ttccgtacca tccggaacat
     2341 gcagaacgtc gccctcacag gaaacaatct ggaatccggt ggctccaggg gtttctgcaa
     2401 gcattgaaga ggtggatgcc aggatgagaa gaagacagag caatccgttg cggaacacga
     2461 tagacataca attctccctg ataccagttg tgttcggtcc agcgcgtatc gccacatctt
     2521 accttcccga gagccgggaa gtccatgttt ctgtacggca ggtcagggtc acgggttttt
     2581 ctgaaaacac ttgttctttc ttcgcatcgc tcgatggcaa ccaggtgagc attccatgaa
     2641 cctgatgtcg tggtcggatg aatacatggc gagagggaga cgccgtgctc tggcatgcgc
     2701 ctgggttttc ctgtcgagca gacgcaattc ttctggtgta tctggtaccg ggtggtgcgg
     2761 tcgtgtatga ttcagtcgtt ctctttctta tcttgcctgt ggtcggagga gttgtgaacg
     2821 tttccccccg aaaagtctgc tggctttcgc ttctggtttt tctggcgatt tctctcgcgt
     2881 gtcgcgccca ggcggtatcg cctgtcgctg cggcatcgga gacgcatctc gttcccggag
     2941 cttccactac gacgatgccg gtattcgatg cggcgagtcg atctctggag acaaagatcg
     3001 actctgacat cgcgatcatt gccccggttg cctcggaagc gtctcgatca tggttcattg
     3061 cggctgtggg cgacatcatg gtgcatgaga cgcaattgaa aacgcattgg gacgccggcg
     3121 cgaaacagta tctcttcgac gacgatttca tccatattcg accacttctc gccagcgccg
     3181 acctgatgat cggaaacttc gaaaccacgc tgggcggccc ggggcgcaag tatagcgggt
     3241 atccggtctt caacacgcct gacacgctgg ctgaagccct gaagcgggcg agtttcgacg
     3301 ttctgatgac ctcgaacaat cactgtctgg acacaggctt tgccggcctg aaacgtacgc
     3361 atgcggtatt gtccgggttg ggttttggtg tcattggcac caaaaacgat gaaaaggcgg
     3421 aaagtttcct ggtgcgagag ctgaacggtc tgaaagtcgg cctggcagcc tacacgtatg
     3481 agacgtcgcg caagcaagga aaggcgacgt tgaacggggg actgatgccc cccggagcct
     3541 cgacgctgat caactcgttt gctaccgaaa cgctcgaagc aaatctcgcg agcatgagcg
     3601 aacgcatcag aatcctgcgt tctcgcgggg ccgagctcat cgtgttcgtg ctgcactggg
     3661 gagtcgaata cagaccgcag ccgactcc
//
LOCUS       DAORHD010000362         3679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27719_length_3679_cov_3.330298, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000362 DAORHD010000000
VERSION     DAORHD010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27719_length_3679_cov_3.330298"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1394
                     /locus_tag="PKO06_08810"
     CDS             3..1394
                     /locus_tag="PKO06_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV69784.1"
                     /translation="MPILLVDDEVEILRGFEALLATAGLESVITCQDPREVPGILAKQ
                     EVGVLLLDLWMPGLSGEQLLVQVAESWPDVPVIVVTGVNEVQTAVRCMRQGAFDYLVK
                     PVDEMRLISSVQRAVDLRQWRDDYARMRRSLLQRRLEHPDAFAGIVTRSPRMLAVFQY
                     IESVAPSHQPVCITGETGTGKELVARALHTLSAPKGPFTAVNVAGLDDMVFSDTLFGH
                     VRGAFTGADAKRSGMIEQAAGGTLFLDEVGDLSLASQTKLLRVIQQREYFPLGSDVPR
                     PVRARIVAATNRHLETLRDAGTFRRDLYHRLTVHQVEIPPLRERMEDLPLLLRCFQDR
                     VETETGKEQPRWGTDLIALCERYAFPGNVRELESLVSDVTAMSARGDSTKHLLLSRLR
                     LSGETVPEPAGVSDLLRFGDERFPTLDQVEEAMIHEAMRRSDGNQSMAARLLGISRPT
                     LCKRLKKTPPVRL"
     gene            1799..2272
                     /locus_tag="PKO06_08815"
     CDS             1799..2272
                     /locus_tag="PKO06_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69785.1"
                     /translation="MRNQCVCRDRQTATGKCLSFEMPASEANIQVIVNCALHFLQGEG
                     MLEQHAVAVVMRELLANAVVHGSATRSDARVRGEMTVKHGGSIEIWVEDEGRGFDYQA
                     LDLRLPADQPDSLRQRGLILVKALVQDLSWNEPGNRVTVQLHSEGERRGKHGSSR"
     gene            2256..2783
                     /locus_tag="PKO06_08820"
     CDS             2256..2783
                     /locus_tag="PKO06_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV69786.1"
                     /translation="MAHQGESGGKERGLRAQAGKYLTFKLGGGDYGIEIIKVVEIIKL
                     MPITQVPRMPDFIRGVINLRGKVIPVMELRRRFGMEHVDDSSNTCIIVVGLRTKSGEV
                     QMGTLVDTVSEVLDVAAVDIEPAPAFGGGAGVDTSFILGMAKTKGTVRILLDIDRILN
                     AEELDQVSEIPTGAK"
     gene            2796..>3679
                     /locus_tag="PKO06_08825"
     CDS             2796..>3679
                     /locus_tag="PKO06_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HNV69787.1"
                     /translation="MFGNRTLAFKIGFGYVVLLAIAIFLGALAIVKMTGVTTEATKLQ
                     AEYVPEVEVANNVERHSLMVMYAIRGYGYTEEPKFLEDGKKELGEVHKYLDAATALAD
                     KSANLKILKENAAKVKTEVQNYEKLVEDTKNQVGNLEELRKKMGEEAKAFMDNCYAFI
                     AGQNEALTKEVNEKAEPEKMLERIHKIALANDIVDLGNEVRILNWQSQATRDPAKIQK
                     AMPEFDKIVEKLEELRKITRQEANLKQIEETKKAGLDYKNAMSTFLKDWLDLQEIAKR
                     RTETAGKVVAGAQETALGG"
BASE COUNT          834 a          950 c         1151 g          744 t
ORIGIN      
        1 agctgcccat tctgctcgtg gacgatgaag tggagattct gcgtggtttt gaagccctgt
       61 tggcgaccgc cggtctcgag tcggtgatca cctgccagga tccacgagaa gtcccgggga
      121 ttctggcgaa acaggaggtc ggagtgctgc tgctcgacct ctggatgccg gggctttccg
      181 gcgaacaatt gctggtacag gtggccgagt cgtggcccga cgtgccggtc atcgtcgtca
      241 ccggggtgaa tgaagtgcag accgcggtgc gctgtatgcg ccagggggcc tttgattacc
      301 tcgtgaagcc ggtcgacgaa atgcgcctga tctccagtgt gcaacgtgcc gtcgatttgc
      361 gtcagtggcg cgatgattat gcccggatgc gacggtcgct gttgcagcgc agactcgaac
      421 atcctgacgc gttcgccgga atcgtcaccc gcagcccacg aatgctggcg gtgtttcagt
      481 atattgaaag cgtggctccc agtcaccagc cggtgtgcat caccggagaa accggtaccg
      541 gcaaggaact ggtcgcgcgg gctctgcaca ccttgagcgc gcccaagggg ccattcaccg
      601 cggtgaacgt ggctggcctc gatgatatgg ttttctctga tacgttgttc gggcatgtgc
      661 gtggcgcttt taccggagct gacgcgaaac gatcgggaat gatcgagcag gctgccggcg
      721 gcaccttgtt cctcgacgaa gtcggcgatc tttcactcgc ctcgcagacc aagctgctgc
      781 gggtgattca gcagcgagag tattttccgc ttgggtccga tgtgccgcga ccggtgcggg
      841 ctcggattgt cgcggcgacg aatcgccatc tcgagacgct tcgcgacgcg ggaacgtttc
      901 gtcgcgactt gtaccatcgc ttgaccgtgc atcaggtcga gattccaccg ctgcgcgaac
      961 gcatggaaga tctgccactc ctgctgcgct gtttccagga tcgtgtcgag accgagaccg
     1021 gaaaagagca gccacgctgg ggaacggatc tgatcgcact gtgcgaacga tatgcctttc
     1081 cggggaatgt ccgtgaactc gagtcgctcg tcagtgatgt cacggcgatg agcgcccggg
     1141 gagactcgac aaaacatctg cttctgagtc gattgaggct cagcggcgag acggttcctg
     1201 aaccggccgg agtgagtgac ctgctgcgtt tcggcgatga gcgctttccg acacttgacc
     1261 aggtcgaaga ggcgatgatt cacgaagcca tgcgccgatc ggacggcaat cagtcgatgg
     1321 ctgcccgcct gcttggcatc tctcgcccca ctctctgcaa aagactgaag aaaaccccgc
     1381 cggttcgact ctgaaccgct gccgtcccct ctgggttctg taagtttcgc tggtgccagc
     1441 tcttatgaat gagagggaag tgttcagatc gaagcacgag cgtgacctga gaggaactgt
     1501 tccgggaagt ccggagatct gacgcacggc aaggatcaac ctgttcgttt ttttacaggt
     1561 tgcccttttt cacacctgaa tcatcggccc gactggcagc cgttctgtac cacaggcgcg
     1621 atctcctgta tccactgccc ctgttcgatt ttttacagtc gagtaacacc tgaaaaacgt
     1681 cctgacaggg gctgctgagg cgtagggaag agcacagcaa ggcggttggt actggccttg
     1741 ctttgctggt tggtgagctg aaaaacgggt ttccagtcga gatgcaaggg tggtggggat
     1801 gcgcaaccag tgtgtctgca gagatcgcca gacggcaacc ggaaagtgtc tgtcgttcga
     1861 gatgccggct tccgaggcga acattcaggt catcgtgaac tgtgctctcc attttctgca
     1921 aggcgagggc atgttggagc agcacgcggt tgcggtggtc atgcgcgagt tgctggccaa
     1981 cgcggtcgtg cacgggagtg ccacccggag cgacgcacgt gtgcgcggtg aaatgaccgt
     2041 gaaacacggc ggctcgatcg agatctgggt tgaagacgag ggccggggtt ttgactacca
     2101 ggctctcgat ctgcgtctgc cggctgatca acccgacagt ttgcgacagc gtggattaat
     2161 cctggtcaag gctttggtcc aagacctgtc ctggaacgag cccgggaacc gggtgacggt
     2221 gcaacttcac agcgaaggcg aaaggagggg gaagcatggc tcatcaaggt gaatccggag
     2281 gcaaagaacg agggttgcgg gcacaggcag ggaagtactt gaccttcaag cttggtggcg
     2341 gagattacgg catcgagatc atcaaggtcg tcgagatcat caagctgatg ccgattacac
     2401 aggttccgcg catgcctgat tttattcgcg gcgtgatcaa tctgcgcggc aaggtgattc
     2461 ccgtcatgga actgagacgt cgtttcggca tggaacatgt cgacgattcc tcaaacacct
     2521 gtatcatcgt cgtcggcttg aggacgaaga gtggggaagt acagatgggt acgctggtag
     2581 acacggtttc cgaggtgctc gatgtggcag ccgttgacat cgagcccgcc ccggcattcg
     2641 gagggggcgc aggagtggat acctctttca ttctcggcat ggccaagacg aaggggacgg
     2701 tccgcatcct gctcgacatc gatcggatcc tgaacgcgga agaactggat caggtttcag
     2761 aaatccccac cggggcgaaa taacaggagg acattatgtt tggaaatcgg acactggctt
     2821 tcaaaatcgg ttttggatac gtggttctgc tggcgattgc catttttctg ggcgcattag
     2881 ccatcgtgaa aatgaccgga gtcacaaccg aggcgacgaa acttcaggct gaatatgtac
     2941 cagaagttga agtcgcaaac aacgttgaac ggcacagttt gatggtcatg tatgccattc
     3001 gcggctatgg ctataccgaa gaaccgaagt tcctcgaaga cggaaaaaag gaactgggcg
     3061 aggttcacaa gtatctcgat gccgcaaccg ctctggcaga taagtccgcc aatctgaaga
     3121 tactgaaaga aaatgccgcg aaggtgaaga ccgaggtgca gaactacgag aagctcgtgg
     3181 aagacacgaa aaatcaggtg ggaaatctcg aagaactccg aaagaagatg ggcgaagagg
     3241 cgaaagcttt catggacaac tgctacgcgt tcatcgccgg gcagaatgaa gccctgacca
     3301 aggaagtgaa cgaaaaggca gaaccggaga aaatgctcga acgcattcac aagattgctc
     3361 tggccaacga catcgtcgac ctgggcaacg aagtgcgaat tctgaactgg cagtcgcagg
     3421 cgacccgcga ccccgcaaag attcagaagg ccatgcctga attcgacaag atcgtcgaaa
     3481 agctcgaaga gctgcgcaag atcactcgtc aggaagccaa tctgaagcag atcgaagaaa
     3541 cgaaaaaagc cggtctggat tacaaaaatg cgatgagtac gttcctgaag gattggcttg
     3601 atctgcagga gatcgcgaaa cggcgcacgg aaaccgcggg caaggttgtc gccggcgcgc
     3661 aggaaaccgc actgggtgg
//
LOCUS       DAORHD010000363         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27755_length_3675_cov_3.994751, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000363 DAORHD010000000
VERSION     DAORHD010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27755_length_3675_cov_3.994751"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="dxs"
                     /locus_tag="PKO06_08830"
     CDS             complement(<1..1233)
                     /gene="dxs"
                     /locus_tag="PKO06_08830"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNV69788.1"
                     /translation="MNLEQIRSPKDLRGLTHAELHDLSAQIRERIIQIVSRNGGHLAS
                     NLGIVEMTLALHRVFESPQDRIIFDVGHQCYTHKLITGRIDPFPTLRTLGGLSGFPKM
                     CESPHDAFDTGHSSTSISAALGMALARDHFDQAHKIVAVIGDGAMTGGMAFEALNYGG
                     HKRSPFIVILNDNAMSISPNVGGLSHYLHKIRMEPVFTTPKEYLSHVVRQIPGFGSRV
                     YQILSRLEGSLKYLLTPGSLFEELGFRYLGPIDGYDFALMEQTFAHARQCAEPILIHV
                     LTEKGRGYKPAQDKLPQFHGVGPFEITTGEIIKEKAPPSFSSVFGQALVDMARTDSRI
                     VAITAAMKEGTGLAAFAQHFPERFYDVGIAEQNAITMAAGMAAAGLRPYVAVYSTFLQ
                     RAYDQVIHDVALMNLPVTF"
     gene            complement(1434..1628)
                     /locus_tag="PKO06_08835"
     CDS             complement(1434..1628)
                     /locus_tag="PKO06_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HNV69789.1"
                     /translation="MTTTITGDTIIADILKIRPDAAQILMRHGMHCIGCSVSTAESLK
                     EAAQVHGIDLDKLITDLNTR"
     gene            complement(1707..3158)
                     /locus_tag="PKO06_08840"
     CDS             complement(1707..3158)
                     /locus_tag="PKO06_08840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNV69790.1"
                     /translation="MSSRHHWRLKFRVTHSAEWLFGLFLACADALVLNLIFINTFSFW
                     LQEHASQLSVYLTPYVQVRSWLFLLYICFGLAFGLFSIRALRGAADTLSHAAGTLLST
                     FVTFNLLISLWRPLAVLVYHFPRPIILLSTGFGIMGVFLLRVMLMRFFIPEPQLIRAV
                     IVGDETEGKRILKHFHHRGGIRCRLMGVFAPEQWKEVAETVVFHHVSEVIVTDPHTPL
                     DSFWAQIFYKRRVEPHPFRVRVVFDPAAAGSRVGLASLEDLPLQTIPALPVSGMLRLL
                     KRGFDIAFSLFAILISSPVMLLAALAVRLDSPGPIFYKQRRVGRYGKEFDVIKFRSMY
                     SGAEKGSGPVVATADDPRTTRIGRLIRKLGIDELPQFFLVLTGDMSVVGPRPERPYFV
                     DQHPEFQGRRLAVRPGVTGLAAVNARYYLRLTDKVAYDTYYLDNYHLILDVKIVFQTV
                     WVLLFESEKALHDKHHPLDHLPTPPDDPPQKNT"
     gene            complement(3197..>3675)
                     /locus_tag="PKO06_08845"
     CDS             complement(3197..>3675)
                     /locus_tag="PKO06_08845"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV69791.1"
                     /translation="IAWLAIKIEENGYAYYTRMATQVQKSNVKRLFELLAVAEQQHIV
                     DFTKIREQLPDQPYEIPEEYNSPEIEGYLRSLADGKVFPNLGNVDDLAREIKTEEAAI
                     QHAISFEKDAIIFFSEIYDMLPADMPERGAVAQLIHQEKIHIAKLYILLGNTPVDH"
BASE COUNT          837 a          953 c         1160 g          725 t
ORIGIN      
        1 gaacgtcacg ggcaggttca tgagcgcgac gtcgtggatg acctgatcat atgcccgctg
       61 caggaaggtg gaatacacgg cgacgtaggg gcgcaacccg gcagcggcca tgccggcggc
      121 catcgtaatc gcgttctgct cggcgatgcc gacgtcgtag aaccgttcgg gaaagtgctg
      181 ggcgaacgcg gccagcccgg tgccttcctt catggccgcg gtaatggcga caatgcgact
      241 gtcggtgcgg gccatgtcga ccagagcctg gccgaagacc gaagaaaacg agggaggggc
      301 tttttccttg atgatctcac ccgtcgtaat ttcgaaaggt ccgactccgt gaaactgcgg
      361 aagcttatcc tgcgccggtt tatagcctcg ccctttttcg gtgagcacgt gaatcagaat
      421 cggttcggcg cactggcgcg cgtgggcgaa cgtctgttcc atcagcgcga aatcgtaccc
      481 gtcgatgggc ccgagatacc ggaacccgag ctcttcgaag agactgcccg gagtgagcag
      541 atacttcagg ctgccttcga gtcgactcag aatctggtag acgcgcgatc cgaacccggg
      601 aatctgccgc accacatggc tcaggtattc cttgggggtc gtgaacactg gctccatgcg
      661 gatcttatgc agatagtgcg agaggccgcc gacgtttggg gagatcgaca tggcgttatc
      721 attgaggatg acgatgaagg gcgaacgttt gtgaccgccg tagttcaggg cttcgaaagc
      781 catacccccg gtcatggcac cgtcgccgat cacggcgacg atcttgtgag cctggtcgaa
      841 atgatcgcgg gccagagcca tgccgagcgc cgccgagatt gaggtcgagg aatgaccggt
      901 gtcaaacgca tcatgcgggc tttcgcacat cttgggaaag ccggaaagac cacccagggt
      961 gcgcagcgta ggaaagggat cgatgcgacc agtgatgagt ttgtgcgtat agcactggtg
     1021 gccgacatca aaaataatgc ggtcttgcgg tgactcgaaa acccggtgca gggccagggt
     1081 catctcgacg atgcccagat tgctggccag atgcccacca ttgcgggaaa cgatctgaat
     1141 gatgcgctca cggatctgag cgctcagatc gtggagttcg gcgtgagtca gtccgcgcag
     1201 gtctttcggg gatctgatct gttcgaggtt catgggcacc ggctccggat gtgtggccga
     1261 actgcggcgt cgagcggcag caggcggccg aatatgagga ggtatcgtat cacagggcct
     1321 ctggaaaggc aaaaagagaa agaccggtat cgacgactcg aagcgattcc agtcccctgc
     1381 aggggccgtc gttcacgtgt cgccagttcc cggttcgagg gcggggtttc tgcctatctg
     1441 gtgttcaggt cggtgatcag cttgtcgaga tcgatgccgt gaacctgagc cgcttctttc
     1501 agactttcgg cggtcgatac cgaacagccg atgcagtgca tgccgtggcg catgagaatc
     1561 tgggcggcat ctggtctgat cttgaggata tctgcaatga tggtgtcgcc ggtaatcgtc
     1621 gtggtcataa ctgctccttg catcgtgaga tcatggattg ggaaaaagtt agattagtct
     1681 aataataggc gggttgtgag gaaaaatcaa gtgttcttct ggggtgggtc gtcaggaggc
     1741 gtgggcaggt ggtcgagcgg gtggtgtttg tcatgcagag ctttctctga ctcaaaaagc
     1801 agcacccaca ccgtttgaaa aacgattttc acatcgagaa tgaggtggta gttgtcaagg
     1861 tagtaggtat cgtaggcgac cttgtcggtg agacgcagat agtaacgtgc gttgaccgcc
     1921 gcgagaccgg tgacgcccgg gcgcacggcc aggcgtcgcc cctggaactc ggggtgctgg
     1981 tcgacaaaat atgggcgttc cgggcgcggc ccgacgacgg acatgtcgcc ggtgagaacc
     2041 agaaaaaact gcggcagttc atcgatgcca agcttgcgga tcaggcggcc gatgcgcgtg
     2101 gtgcgcggat catcggccgt ggcgaccacg ggaccgctgc ccttctcggc accggaatac
     2161 atggagcgaa acttgatcac atcgaattct ttgccgtaac gaccgacccg acgttgttta
     2221 tagaagatgg gccccggaga atcgagtcgc accgccagag cggccaacaa catgactggt
     2281 gaggagatga gaatggcaaa taaggaaaag gcgatatcga agccgcgttt cagaaggcgt
     2341 agcattccac tgacaggcag agctggaatc gtctgcaggg ggagatcttc gagactggcc
     2401 agaccgacgc gactgccggc cgccgcgggg tcgaacacga ctcgtacgcg aaacggatgc
     2461 ggttctacgc gacgtttgta gaaaatctgc gcccagaacg agtcgagcgg cgtgtgcgga
     2521 tcggtgacga taacctctga gacatgatga aacaccaccg tttccgcgac ctctttccat
     2581 tgctcgggcg cgaagacccc catcaaacgg cagcgaatgc cgccgcgatg atgaaagtgt
     2641 ttgagaatcc gcttcccttc tgtctcgtca cccacgatga ccgcgcgaat cagctgcggt
     2701 tcgggaatga agaaacgcat caacatgacg cgcagaagaa agacgcccat gatgccgaac
     2761 ccggtcgaca ggagaatgat gggccgggga aagtggtaga ccagcaccgc gagagggcgc
     2821 cagagcgaga tcagcaggtt gaaagtgacg aacgtcgaga gaagggtgcc ggcagcatgc
     2881 gagagagtat cagccgctcc gcgaagagca cgaatcgaga acaatccaaa ggcaaggccg
     2941 aagcagatgt agagcagaaa cagccacgaa cgaacctgga catacggagt cagatacacc
     3001 gacagttgcg atgcgtgttc ctgcagccag aacgaaaagg tgttgataaa aatcaggttc
     3061 aacaccagcg catccgcaca ggccagaaac aacccgaaca gccactcggc actgtgagta
     3121 acgcgaaact tcaatcgcca gtggtggcga gagctcatga cgatgttcct gtggtcagat
     3181 cactcggact cttgcctcaa tgatcgacgg gagtgttgcc aagcagaatg tacaacttgg
     3241 cgatgtgaat cttttcctga tgaatgagct gtgccacggc gccgcgttcg ggcatgtcgg
     3301 cgggaagcat gtcgtagatc tcgctgaaga aaatgatcgc atccttttca aacgagatgg
     3361 cgtgctgaat ggccgcttct tcggttttga tctcgcgggc gaggtcgtca acattcccga
     3421 gattcggaaa caccttgccg tccgccagcg atcgcaggta cccctcgatt tccggtgagt
     3481 tgtactcttc gggaatctcg tagggttgat caggcagttg ctcgcgaatc ttggtaaagt
     3541 ctacgatgtg ctgctgctcg gcaacggcga gaagctcgaa cagtcgtttc acattcgatt
     3601 tctgcacctg cgtggccatg cgggtatagt aggcgtagcc gttttcctcg atcttgatgg
     3661 ccagccaggc gatca
//
LOCUS       DAORHD010000364         3675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27756_length_3675_cov_3.530663, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000364 DAORHD010000000
VERSION     DAORHD010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27756_length_3675_cov_3.530663"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1617)
                     /locus_tag="PKO06_08850"
     CDS             complement(181..1617)
                     /locus_tag="PKO06_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV69792.1"
                     /translation="MDLRTSHIIDVWRSFSGEPDVAKLLQVVVHTAEAVTGAQTGFVI
                     LEGKSWSRLEYRFAAEEEIRMLQMLHQRLGTPVIGIITRNSVKLKVDKPDSTWEVSRT
                     LDPERQLSQKIEIGDQHLGTLYLSGKPGGFTNEDQEFLTAFIDQVKAAIERQLYRERM
                     MKMERRLNAIFDAIADGILVLDQEGLPVMWNRSFGNMFFPDGVENHALSSMLPALIDS
                     EKDQGVQEIVLLKPHEEVVSGHFVVTRNETGKKMETVVSLRNVTQIRRQEQRFLQLMA
                     MVVHRIHKRLRAIPPCLRRKGLNRYRRIRRLTRNLIYLTEIKSGPLRISKGPNRLGDL
                     VQMIEQRAKRMFTRRNLALNISRQIDEKAFLTVDSGILKDAFSAVFSFVARKLKAEST
                     CTIRLFSEAEKIKIDVRAPFECWKVLPNPDHLNWLIAIDYFLAEDKRPFLLEMPFARH
                     IFESHKGALDLVSDSHDAGLTMMVPLEI"
     gene            1794..3572
                     /gene="aspS"
                     /locus_tag="PKO06_08855"
     CDS             1794..3572
                     /gene="aspS"
                     /locus_tag="PKO06_08855"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV69793.1"
                     /translation="MHTYRTHTCVELRQEHLNQHVRLSGWIHRKRNLGGMVFIDLRDH
                     HGITQILIEPNTEPYATVEPLRHESVITVDGVVQSRGAGNINERMPTGAIEVRATSVS
                     VQSSAEILPFPVSGEPLTVPENTRLTYRFLDLRRQKLHQNIVLRSKIISSLRRRMTDL
                     GFLEYQTPILTSSSPEGARDFLVPSRIHPGKFYALPQAPQQFKQLLMVSGFDRYFQIA
                     PCFRDEDARADRSPGEFYQLDVEMSFVTQDDVFSTIEQVMHGVFSEFSSHEITSTPFP
                     RIPFEEAMRKFGSDKPDLRFELHVQDVTDLFATGDFKVFREVAAQGGVVRCLSVPGVG
                     AKSRKFFDDLEAFARENGAKGLASLVFTGGQVKGSLAKGLSEGLRNQLKQRTATGDDA
                     AILLMADQAATVASVLGKVRLNLADQLDLRQKNCYKFCWITDFPMFERDPESGQIIFS
                     HNPFSMPQGGMEALCTQDPLTIKAFQYDIVCNGIELSSGAIRNHRPDIMYKAFEIAGY
                     KKEDVDAKFGGMIKAFTFGAPPHGGIAPGVDRMVMLLADEPNIREVILFPMNQQAQDL
                     LMGAPCDVTEKQLKELHIQIVPPTKD"
BASE COUNT          821 a         1044 c          982 g          828 t
ORIGIN      
        1 attgcattcg acaaggcctc atcgatgcag agaagaatct gatactcgat gcgacgctcg
       61 agcttgtact gttcgagttc ggcgcgcaga tcacgccgca ccagctccgc agcctgcatc
      121 ttcactggta tccgatgcac tttggcgtag gtgatgggct tcacttgttt cacgttcata
      181 tcagatctcg agtgggacca tcatggtcag accggcatca tgagagtcgc tgaccagatc
      241 gagagcccct ttgtgagatt caaaaatgtg gcgggcaaac ggcatttcga gcaggaacgg
      301 tcgtttgtct tcagccagaa agtagtcgat cgcgatcaac cagttcaggt ggtccgggtt
      361 cggaagcacc ttccagcatt caaatggcgc tcgcacatcg atcttgatct tttcggcttc
      421 cgagaacagt cgaattgtac aagtcgattc agccttgagt ttgcgcgcca caaaagaaaa
      481 gaccgccgag aaggcgtctt tgagaattcc ggagtcaacg gtgagaaaag ccttttcgtc
      541 aatctggcga ctgatattca gagcaaggtt gcgccgcgtg aacatacgct tcgcccgctg
      601 ttcgatcatc tgtaccagat cgccgagtcg attgggacct ttggatattc gcagaggccc
      661 ggacttgatt tctgtcagat agatgagatt tcgggtcaga cggcgtatgc gccggtaccg
      721 attgagaccc ttgcgccgca ggcagggcgg aatcgctctc agacgcttgt ggatacgatg
      781 gaccaccatc gccatcaact gcagaaaccg ctgttcctgt cgccggatct gagtcacatt
      841 gcgaagcgag acgacggttt ccatcttttt cccggtctcg ttacgtgtca caacgaaatg
      901 acctgacaca acctcttcat gaggtttcag cagtacgatc tcttgtactc cctggtcttt
      961 ttcgctatcg atgagggccg gcagcatcga ggacagcgca tgattttcca cgccatcggg
     1021 aaagaacatg tttccgaagg agcgattcca catgactggc aacccttcct gatcgagcac
     1081 caggatacca tcggcgatcg catcaaagat ggcgttcagt cgccgctcca tcttcatcat
     1141 ccgttcccgg tacaactgcc gttcgatcgc ggccttgacc tgatcgataa atgcggtaag
     1201 aaactcctgg tcttcattcg taaaaccgcc aggcttgccc gagagataca gcgttccgag
     1261 atgctgatcg ccgatctcga ttttctgcga cagttggcgt tccgggtcga gtgtgcgcga
     1321 aacttcccac gtcgaatcgg gtttgtcgac cttcagtttc acgctgtttc gcgtaatgat
     1381 gccgatgacc ggagtcccca gacgctggtg cagcatctgc agcatgcgaa tctcttcttc
     1441 ggcggcaaag cgatattcga ggcgactcca gcttttgcct tcgagaatca cgaacccggt
     1501 ctgtgccccg gttaccgcct ctgctgtgtg aacaaccacc tgcagcagtt tggcgacatc
     1561 aggttctccg gaaaaggatc gccagacgtc gatgatatgg ctggttcgta aatccatggg
     1621 ccttctacac gcgaaagatc gtgacgcaat gcgcgagcaa tggctattct aacatggagg
     1681 agcaggaccg acaaccaaca caccgcgaag cttgaaaagc cttcctgggt gcggtacact
     1741 gcggccgatt tccctgactt tcgaacccta ttccgccgcg gaggactgat ttcatgcata
     1801 cctatcgaac ccatacctgc gttgaactgc gccaggaaca cctcaaccaa cacgtacgcc
     1861 tctccggctg gatccatcgc aaacgcaatc tgggcggcat ggtgttcatc gatctgcgtg
     1921 accatcatgg catcacccag attttgatcg aaccgaacac ggagccatac gccaccgtcg
     1981 aacccctccg tcacgagtcc gtcattaccg tcgacggggt cgtgcagtca cgcggagcag
     2041 ggaacatcaa tgaacgcatg cccaccggag ccattgaggt gcgggcgacg tcggtcagcg
     2101 tccagagttc cgccgagatt cttccgttcc cggtttcagg cgaaccgctg acggttccgg
     2161 aaaacacgcg tctcacctat cgttttctcg acctgcgccg ccagaaactg catcagaaca
     2221 tcgtgttgcg ttcaaagatc atttcctcac tgcgacgccg catgaccgat ctcgggttcc
     2281 tcgagtacca gaccccgatt ctcacgagca gctcaccgga aggagcccgc gactttctcg
     2341 tgccgagccg cattcacccg ggaaaattct atgccttgcc tcaggcacca cagcagttca
     2401 agcaactgct catggtctcc ggtttcgatc gctatttcca gatcgcaccc tgtttccgcg
     2461 atgaagatgc tcgcgccgac cgatctccgg gcgagtttta tcaactcgat gtcgaaatgt
     2521 catttgtcac gcaggatgac gtgttttcga ccattgaaca agtgatgcac ggcgtcttca
     2581 gcgagttcag ctcgcatgag atcacctcga ctccgttccc acgcattccg tttgaggaag
     2641 ccatgcgcaa gttcgggtca gacaaacccg acctgcgttt cgaactgcat gttcaggatg
     2701 tcaccgacct cttcgccacg ggcgatttca aggttttcag agaggtggcc gctcagggcg
     2761 gtgttgtgcg ctgcctgagt gttccgggtg tcggggccaa atcacgcaag ttcttcgacg
     2821 acctcgaagc ctttgcccgt gaaaacggtg ccaaggggct ggcgtctctg gtgtttaccg
     2881 gcggtcaggt gaaaggcagt ctggcgaaag gcctttcaga aggtctgcgg aaccagttga
     2941 aacagcgaac ggcgacgggt gacgatgccg ccattttgct gatggcggat caggcggcga
     3001 cagtcgcatc agttctggga aaagtgcgcc tcaacctggc cgaccagctc gacctgcgtc
     3061 agaagaactg ttacaaattc tgctggatca ccgattttcc gatgtttgaa cgcgatcccg
     3121 aatcaggtca gatcatcttc agtcataatc cgttctcgat gccccaggga gggatggaag
     3181 cactttgtac gcaggatccc ctgacgatca aagcatttca gtatgatatc gtctgcaacg
     3241 ggatcgaact gagttcaggc gctatccgca atcatcggcc cgatatcatg tacaaagctt
     3301 tcgagattgc cgggtataaa aaggaagatg ttgacgcaaa gttcggtggc atgatcaaag
     3361 cctttacctt cggcgctccg ccgcacgggg gtatcgcgcc tggcgtcgat cgcatggtga
     3421 tgctgctggc cgacgaaccg aacatccgcg aggtcattct gttcccgatg aatcagcagg
     3481 cccaggatct cctcatggga gctccgtgcg acgtcaccga gaaacaactg aaagaactgc
     3541 acattcagat cgtcccaccg acaaaggact gaacttcgct gcggatgatt gtaagactgg
     3601 gcaagaaacg gaaatcaata gccgatgaca agtgactggc agggtctact ggcccggctg
     3661 cgctggctgg tcacc
//
LOCUS       DAORHD010000365         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27781_length_3673_cov_3.100608, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000365 DAORHD010000000
VERSION     DAORHD010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27781_length_3673_cov_3.100608"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKO06_08860"
     CDS             <1..287
                     /locus_tag="PKO06_08860"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006399 - tRNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNV69794.1"
                     /translation="GKMNLSVLDLGLEILVVSQFTLFGDVRKGFRPSFTESEAPEPAC
                     LLYERFMDGLRQAGVRRVCGGVFAADMRITQENRGPVTILLDSRDKHTLG"
     gene            complement(419..835)
                     /locus_tag="PKO06_08865"
     CDS             complement(419..835)
                     /locus_tag="PKO06_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69795.1"
                     /translation="MELTMYGKLARALQEYFSAGDDTAILIVGFIVALGLLIIILQSA
                     LSSRETETVASDKDLDFFEVVIQQKGLENFDRDLLLELAEVGNIHPIYRILIEVQAYE
                     RALRKMEKEIHARLRNADEIPIRLDYLRRLKKRLFQ"
     gene            complement(849..3341)
                     /locus_tag="PKO06_08870"
     CDS             complement(849..3341)
                     /locus_tag="PKO06_08870"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69796.1"
                     /translation="MIWPFHSARPPQDLIKDLSSGDASTRDRAARELIDHDSPQTDLL
                     LIAALKNSDEEPRPTRLKMIEVIGKRGIEDAVDHLSPFLTEEDRDLRAETLHALIRIP
                     TQTSLDGILPLLIDEDNAIKREARESILRVFGMKAMGAILRAIPDDKTSPLYFELVSL
                     FEEMGFFDVLSENFAHDDEEVKKFHLPNLMKFHRPDFVPMYLSLADTASTTVQAQIRD
                     ALCEYTPDELLPHIEALFRQGVPNRHQLRMIEDCLFQRHPNAKEELLHLVIRIPKNEI
                     RYEFAQKTLRKPDTILFLPALQLLEDPYPAIRQTTGDALMQVIRQTRQRLDDPGEPNK
                     AYLRELIQSWRNRVVEIIKLSPSRDALIALSKVLFALLDAKHLELVIQALPKLFEECF
                     LDTWRSLSSLDADIRNSLLGQALKEDPGLVSQILDMLNRQPNPDLMKLLLKNVGNLTP
                     EHQSSFQKHVSKKSGSYKLRELLDDSDPQMRASTLEFLAQTSFDGINTLLSRAILDPS
                     PLVRQKGLDIAIQRKHPDLAHMTEKAAQDPDPTVALTALRHGKTSIDESRLAPMLAKA
                     VQSPSEELREFALREIARITQKRYVENFNRMPPHVRKLAGSALLKLDTGFVDQLLGDL
                     RSLDTETRLRAAMIFENLQAAGKAREGLLAAMKDPSNKVRAAVVKSLGVLADHSLLSH
                     LIEFLSDPDERVRANTIEAIAAAGNREAANLLVPFLEDANNRIRANAALAVWQIARLN
                     ILPVLTRMLRDKQPVMRASALWVCGEVKQPEHLRLIMSFLNDPNDLVRVNAVKAVMKI
                     RPEVLKPVLPKLRRDSSPEIKKLVAELSFKII"
     gene            complement(3338..>3673)
                     /locus_tag="PKO06_08875"
     CDS             complement(3338..>3673)
                     /locus_tag="PKO06_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69797.1"
                     /translation="NSIVTLWKLGYYQIYESVIEMLRDPDKWMRASAAFSLGELKDMR
                     FFPVLIQCMRDPDADVRRNVVVALGKLADPFVLAPYIRQLRFDPDEKVRKVVQDILST
                     PQKKPQATK"
BASE COUNT          775 a          940 c         1114 g          844 t
ORIGIN      
        1 agggcaagat gaacctctcg gtactggatc tggggctcga gatactggtg gtgtcgcagt
       61 tcactctttt tggcgatgtt cgcaaggggt tccgtcccag ctttactgaa tcagaagccc
      121 cggagccggc ttgtctcctg tatgaacgtt ttatggatgg acttcgacaa gccggggtcc
      181 gtcgtgtctg cgggggtgtc ttcgccgctg acatgcgcat tacccaggaa aaccgtggac
      241 cggttacgat tctgctcgat tccagagaca aacacactct cggttgacgg tgtcccgcac
      301 ccagcgaaca agcgcgacgg ttggggcgag ataaagggcc tgtcacgggc ctgctgagct
      361 gctatgacgc ctctgggacc cgtacgatcg gttggttttt cgtggccccg actttcactt
      421 attgaaacaa tcgtttcttg agtcgacgca ggtaatcgag acggatcggt atttcatcgg
      481 cgttgcgcag gcgtgcgtga atctcttttt ccattttacg cagcgcgcgc tcataggcct
      541 gaacttcgat caggatgcgg taaatggggt gaatgttacc gacttcagcc agttcgagaa
      601 gcagatcgcg atcgaaattc tcgagtcctt tctgttgaat gaccacttca aaaaaatcca
      661 gatctttgtc cgaggcgacc gtttctgtct ctcgcgagga gagcgcgctt tgaagaatga
      721 tgattaaaag gccgagggca acgatgaacc cgacaatgag aatcgcggtg tcatccccgg
      781 ccgaaaaata ctcctgcagg gcgcgggcca gtttgccata catggtcagt tccacattga
      841 gatcctggtc agatgatctt gaagctcagt tcggcgacaa gcttcttgat ttcgggtgaa
      901 gaatcccgcc gcagtttcgg cagcacgggc ttcagaacct cgggtcggat cttcatcacg
      961 gctttcacgg cattgacgcg aacgagatca ttgggatcat tgagaaaact catgatcagg
     1021 cgcaggtgtt ccggctgctt gacttcgccg cagacccaga gcgcgcttgc ccgcataacg
     1081 ggttgcttgt cacgcagcat gcgcgtcaga accggcagaa tgttcaggcg ggcgatctgc
     1141 cagacagcca gcgccgcgtt ggcacgaatg cggttgttcg catcttcgag aaaggggaca
     1201 agcagattgg cggcctcgcg gttgcccgcg gcggcaatgg cttcgatcgt gttcgcccgt
     1261 actcgttcat ccggatcgct caaaaactcg atcaaatgcg acaagagaga gtggtcagcc
     1321 aggactccca ggcttttgac gacggcggct cgaaccttgt tcgacgggtc tttcatggcc
     1381 gcgagtaacc cttcgcgcgc tttccccgcg gcctgcaggt tctcgaagat catcgcggca
     1441 cgcagacggg tctcggtatc gagtgagcgc aggtcaccga ggagctgatc gacgaagccg
     1501 gtatccagtt tcaacaaggc cgaaccagcg agcttgcgca cgtgcggcgg catgcggttg
     1561 aagttttcga cataccgctt ctgggtgatg cgggcgatct cacgcagggc gaactcgcgc
     1621 agttcttcag atggactctg cacggctttg gccagcatcg gggccagtcg actctcgtcg
     1681 atcgacgttt tcccgtgacg cagcgctgtg agagcgacgg tcgggtctgg gtcctgggcg
     1741 gccttttcgg tcatgtgagc caagtcgggg tgttttcgtt gaatggcgat atcgagcccc
     1801 ttctggcgaa cgagtggcga tgggtcaaga atcgcccgac tgaggagagt gttgatcccg
     1861 tcgaacgagg tctgagccag gaactccagg gtcgacgcgc gcatctgtgg atcggagtca
     1921 tcgagaagtt cgcgcagttt gtacgagccg ctctttttgg aaacatgctt ctgaaagctg
     1981 gattgatgtt cgggagtgag attcccgaca tttttcaata acagcttcat caggtccggg
     2041 ttcggttgac ggttcaacat atcgagaatc tgagagacga gtcccggatc ttccttgagc
     2101 gcttgtccga ggagtgaatt acgaatgtct gcatcgagtg acgataagct gcgccaggta
     2161 tcgagaaaac attcttcaaa gagtttgggt aacgcttgaa tgacgagctc gaggtgtttg
     2221 gcgtcgagga gcgcgaacag aaccttggaa agtgcgatca gcgcgtcgcg gctgggtgac
     2281 agcttgatga tctcgactac ccggttcctc caggactgaa tcagttctcg gagataagcc
     2341 ttgtttggtt cgccggggtc atcaaggcgc tgtcgggtct ggcggataac ctgcatcaat
     2401 gcatcgccag tggtctggcg aatggcaggg taggggtctt cgagcagttg cagggcggga
     2461 agaaagagga tggtatccgg ctttcgcagg gttttctggg cgaactcgta gcgaatttcg
     2521 tttttcggaa tgcgaatgac cagatgcagc agttcttcct tggcgttcgg atgccgctgg
     2581 aacagacagt cttcgatcat acggagctgg tgccggttgg ggacaccctg ccggaacaac
     2641 gcttcgatat gcggcagcag ttcgtcgggc gtgtattcgc acaacgcgtc gcgaatctgt
     2701 gcctgaacag tcgttgaggc cgtgtccgcc agactcaggt acatcgggac gaagtcgggg
     2761 cgatgaaact tcatcaggtt gggtagatga aacttcttca cctcttcatc atcgtgagca
     2821 aaattctcgc tgagaacgtc gaagaagccc atttcctcaa agagcgacac cagctcgaaa
     2881 tagagcggcg acgtcttgtc atcgggaatc gcccgaagaa tggctcccat ggctttcatg
     2941 ccgaaaaccc gcaaaatcga ttcacgggct tcgcgcttga tggcgttgtc ttcatcgatc
     3001 agcaacggca gaatcccatc gagcgatgtt tgcgtgggaa tgcggatcag agcatgaaga
     3061 gtctcggcgc gaagatcacg gtcctcttcg gtcagaaacg gggacaaatg gtcgacggca
     3121 tcttcgatgc cacgcttgcc gatgacctcg atcattttga ggcgggtcgg tcgaggctct
     3181 tcgtccgagt ttttcagtgc ggctatcagc agcagatcag tttgaggtga atcgtgatct
     3241 atcagctcgc gtgccgctcg gtcacgagtc gaagcatctc cactgctgag gtccttgatc
     3301 agatcctggg gtggacgtgc ggaatgaaag ggccagatca tttggttgcc tgcggctttt
     3361 tctgaggtgt ggagagaatg tcctgaacga cctttcgaac tttctcatcc gggtcgaaac
     3421 gcaactgtcg aatgtacgga gccaggacaa acggatctgc gagtttgccg agcgcgacga
     3481 cgacgttgcg tcgaacgtcc gcgtcgggat cgcgcatgca ctggatcaga accggaaaaa
     3541 agcgcatgtc tttcagctca ccaagcgaga aggccgctga ggcgcgcatc catttgtccg
     3601 ggtcgcggag catctcgatg acggattcat aaatctggta atacccgagt ttccagaggg
     3661 tgacgatact gtt
//
LOCUS       DAORHD010000366         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27789_length_3672_cov_4.115012, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000366 DAORHD010000000
VERSION     DAORHD010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27789_length_3672_cov_4.115012"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2344)
                     /locus_tag="PKO06_08880"
     CDS             complement(140..2344)
                     /locus_tag="PKO06_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69798.1"
                     /translation="MIPAVEVRGVRSAFLLIVVPVIALIVLAWSLIGILQNEKELAAT
                     VSRGTIFFEALSDSVTHLQRERGKTMLFLEHQLSEPELREQYLATDEACRTLERVLGE
                     GYPSGDVVRVLRDDLAAVKPAREQMLNRAWPADQVFDFYGTRIQGFLLQERSALTADS
                     RGEYALALNSIFWLEAMREQAGRLRARGARLMARPSANASREHEMLLGNPFVCPQLQE
                     MTLLMLDADSRAELDKRRRGESWTVVSSMLDELLRASDGNSSRGDPKVFFQAMTSLID
                     DLDQVIRHELRHLRELETESFLAAGTRIDRVRTLVWLVLAYLGIMGVFLYRRWCIALE
                     SARRLEELRAQKELILSAAGEGIFGLNADGTFSFVNPSALTMLGYSADEIIGSYSHTL
                     IHHHYPDGREYPAEECPIYRAWRCGKVERRDSDVFWKKDGTALEVEYISTPISDSSGI
                     QGAVVVFSDITVRRRLERDLREALQELETAQEQESRIGFQIQEALLFGNPPQGMPDLH
                     IARLAIPAQGVDGDFFNFVSLDDETLDILVGDVMGKGIAAALVGAATKSQFQLALAES
                     GGPGVSPTPQTVVTRVCRHMSQPLSQVNRFVCLHYGRIHRKERRLVLVNCGHTPLVHF
                     CAETSRCQQFFPENPPLGTEEGRPFPETTVPYAQGDLFVFFSDGITEARDADRVMFGT
                     ERLLTLIERHAPHGADAVAEAIRAAVQTHTGAVPVQDDVTCIVIEARRFSGA"
     gene            2623..>3672
                     /locus_tag="PKO06_08885"
     CDS             2623..>3672
                     /locus_tag="PKO06_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HNV69799.1"
                     /translation="MSQPGYYRFPTLFQDQITFVSEDDLWTVSASGGTARRLTSGLGA
                     IQYPSYSPDGRWLAFSGRDEGPLEVFLMPAEGGQPRRLTFLGTQSRVIGWKNPREIIF
                     SSNAGQPFYADFRLFTISLDGGQPRRLPWGPGFSLSFGPQGACAIGRNAVDPARWKRY
                     RGGTAGEILIDVSGTGHFRKLVKTGGNLAYPMFIGSRVFFLSDHEGVGNLYSCRPDGS
                     EIRRHTHHEVFYVRHPASDGARIVYHAGADLYLFDPKRKQTERIAVDYHSPRTQLFRR
                     FIDASVYLEDAHQHPQGHLLGLIVRGKPVTLGNWNGPVQQFGNAEGVRYRLFRWLKDG
                     RRFVVCSDEPGEECLE"
BASE COUNT          774 a         1132 c         1032 g          734 t
ORIGIN      
        1 gcgcacacgc gcgatgctat tgtatcagag cgaagaactc ggcgagcacc agctcgaaaa
       61 tcacggagaa caaacaagaa agcagcctgt tcacgcggag agggagctca tctgacgcga
      121 cgatggccgc cgcagtacgt caggctccgg aaaagcgtct cgcctcgatc acgatacagg
      181 tcacatcatc ctgcaccggc acggctccag tgtgtgtctg tactgccgct cgaatagcct
      241 ctgcgacggc atcagctcca tgcggagcat gtcgttcgat caaagtcagc aaacgctcgg
      301 ttccgaacat gacgcgatcg gcatctcgcg cctcggtaat tccgtcagag aaaaagacga
      361 acaggtcacc ttgagcatac ggcaccgtcg tttcaggaaa tggcctgcct tcttccgtgc
      421 ccagaggcgg gttttcggga aagaactgct ggcagcgcga tgtttctgcg caaaaatgca
      481 ccagtggcgt gtggccgcag ttcaccagaa cgaggcgcct ctcttttcga tgaatacgac
      541 cgtaatgcag gcaaacgaag cggttcacct gtgacaacgg ctgggacatg tgccgacaga
      601 cccgggtgac gacagtctgt ggtgtcgggg agaccccagg tccaccggat tcggccagag
      661 cgagctgaaa ctgactcttc gtcgccgcgc cgaccaaggc tgccgcgatc cccttgccca
      721 tgacgtcgcc aacaaggatg tcgagtgtct cgtcatcgag gctcacgaag ttgaagaaat
      781 caccatcgac accctgcgcc ggaatcgcca gacgggcaat atgcagatcc ggcattccct
      841 gcggcggatt accgaacagc agcgcttcct gaatctgaaa gccaatccga ctttcctgct
      901 cctgagcggt ttccagttcc tgcagggcct cacgcaggtc tcgttcgaga cggcgtctca
      961 ccgtgatatc ggaaaagacg accaccgcac cctgaattcc cgaggaatcg ctgatcggcg
     1021 tgctgatgta ctcgacctcg agagccgttc catctttctt ccagaagaca tccgaatcgc
     1081 gccgctcgac ttttccgcat cgccacgctc gataaatcgg gcattcttcg gcaggatact
     1141 cgcgaccgtc cggatagtgg tggtgaatca aggtatgtga gtacgagccg atgatttcgt
     1201 cagcgctgta gccaagcatt gtgagagctg acgggttgac gaacgaaaag gtgccatccg
     1261 cgttgaggcc gaagattcct tctcctgcgg cactgagaat cagctccttc tgtgcccgca
     1321 attcttccaa tcgccgcgcc gattccagag cgatgcacca gcgacgatac agaaaaactc
     1381 ccatgatgcc gagataggcc agaaccagcc acaccagtgt ccgaacccga tcgatgcgag
     1441 tgccggctgc caaaaaactt tccgtctcca gttcacgcag gtgcctgagc tcgtggcgga
     1501 tcacctgatc caggtcgtcg atcagcgacg tcatggcctg aaaaaacacc ttgggatcac
     1561 ctcgggaaga gttgccgtcg ctcgctcgca gaagctcgtc cagcatcgac gacactacgg
     1621 tccagctttc cccgcgacga cgtttgtcca gttccgcccg actgtcggca tcgagcatga
     1681 gcagcgtcat ttcctgtagt tgcggacaga cgaagggatt tcccagcaac atctcatgtt
     1741 cgcgcgaggc gttcgccgag ggcctcgcca tcaatcgtgc accccgggct cgcagacggc
     1801 cggcctgctc tcgcatggcc tcgagccaga agatcgagtt caacgccagg gcatactctc
     1861 cgcggctgtc tgccgtcaac gcgctccgtt cctgcaggag aaagccctga atccgtgttc
     1921 cgtagaaatc aaacacctgg tccgccggcc aggctcgatt cagcatctgt tcgcgcgccg
     1981 gtttgaccgc agccaggtcg tcccgcagaa cgcgcaccac gtcccctgag gggtatccct
     2041 cacccagaac tcgttccagc gtacggcatg cttcatcagt ggcgagatac tgttctcgaa
     2101 gttccggttc cgacagctgg tgttcgagaa acaacatggt tttcccgcgt tcccgctgta
     2161 gatgcgttac cgagtcggaa agtgcctcga agaagatcgt cccacgcgag acggtggccg
     2221 ccagttcctt ttcgttctgg agaatgccga tcagcgacca cgccaacacg atcaaggcga
     2281 tcaccggcac cacgatcagc agaaatgccg atcggacccc tcgcacctcg actgccggta
     2341 tcatcgttgt ttgcctcgag ttaatgctag tataacagaa tcaatgccga cgcaacaagt
     2401 ttgtttcgat ttcgacgaga ggggcagggt taactcgttc tcgcacaaac ttcgtcggca
     2461 agagcaaaac gcgtgaggct ttcagcacgg gggcttcgga gtccgagggc acgcacccgg
     2521 gtcaccggac gcggaacacc gcgcaatgcc aacgtcggtt gcgtcgaagg cgggggcttc
     2581 ctataatgag gcgttcgcca ccttaccccc aggagccgcc gcatgtctca gcccggctat
     2641 taccgttttc ccaccctctt tcaggatcaa atcacctttg tcagcgaaga tgatctctgg
     2701 accgtttctg cttcgggcgg caccgctcga cgcctcactt caggcctggg ggcgattcaa
     2761 tatccgtcct attcgccaga tggccgatgg ctggcgttca gcggccgcga cgagggcccc
     2821 ctcgaagtgt ttctcatgcc ggccgaaggc ggacaaccgc gacgtctcac gtttctcggc
     2881 acccagtcac gcgtgatcgg ttggaaaaac ccgcgagaga tcatcttcag ttccaacgcc
     2941 ggtcagccgt tctacgccga ctttcgattg ttcaccatct ctctggatgg cggccagcca
     3001 cggcgcctgc cctggggtcc gggattcagt ctgtctttcg ggccgcaagg agcctgtgcc
     3061 atcgggcgaa acgcggttga tccggcgcgc tggaagcggt atcgcggggg aactgccggt
     3121 gagatcctga tcgacgtgag cggcacgggg catttcagaa agctggtcaa aacgggcggc
     3181 aatctggctt acccgatgtt catcggcagc cgcgtgttct ttctgtccga ccacgagggt
     3241 gtcggaaatc tgtattcatg ccggccagac ggcagtgaaa ttcgccgtca cacgcatcac
     3301 gaagtcttct acgttcgaca tccggccagc gatggggcac gcatcgtcta ccacgccggt
     3361 gctgatctgt atctgttcga cccgaaacgc aaacagacgg aaagaatcgc cgtcgattat
     3421 catagcccac gcacccagtt gttccgtcgc ttcatcgatg cttctgtcta tctcgaagat
     3481 gctcaccagc acccgcaggg acacctgctc ggtctgatcg tgcgcggcaa acccgtgacc
     3541 ctcggcaact ggaacggtcc ggttcaacag tttggaaacg ccgaaggggt caggtaccgt
     3601 ctgtttcggt ggctcaagga cggcaggcgg tttgttgtct gctcggacga accgggggaa
     3661 gagtgcctcg aa
//
LOCUS       DAORHD010000367         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27794_length_3672_cov_3.445120, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000367 DAORHD010000000
VERSION     DAORHD010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27794_length_3672_cov_3.445120"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PKO06_08890"
     CDS             <1..540
                     /locus_tag="PKO06_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69800.1"
                     /translation="HLLASCIYHRLPCVTSLGASGKVDPTKVRATDLRKTHDDPLAKT
                     IRKYLWRVYEINLKRVSQLTAVFSDEEPMRPDPEYTGSPCGGAECVCPNSTNQHHTCS
                     ARNIIWGSSVLVTSMFGMTAASLAVRYLAGDESIDLKPDVKTLPGDDSLVDPASRWED
                     GVLKTSPASQPAHETAEDD"
     gene            690..983
                     /locus_tag="PKO06_08895"
     CDS             690..983
                     /locus_tag="PKO06_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69801.1"
                     /translation="MTQSFGFKRLDTDRKHNNGLLFGLDTVIIKDAKRPVTFLADYFG
                     GPAATVGLGLTQNFTPKLNWAVSYYLPTKSKLPVSQTQLPKQLWVALTYYHSL"
     gene            1218..2522
                     /locus_tag="PKO06_08900"
     CDS             1218..2522
                     /locus_tag="PKO06_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69802.1"
                     /translation="MKATDSLARLIWVLVILTGLSADCLPAWDSHFLLTRMATQALGH
                     QVPLLTQEVAPTSLATFVKRNNAALKNTLRAFHDWKQKTYTYPHWKLSEANAALLQGS
                     AIMTPVQFLEILGVSSTAFSKMVFQKEYVDLYGPRYANPHLPRSFGEWLTLFSDEPDW
                     GMDQGLFGTGDPRYGDIPYGDAAGTGSQAPFHMTFPFENWVTYKLKSSLKQGMAHLRF
                     MVFCSLTQVALNAGDHFWAARFLGSAVHYFEDFTMPYHASAVPFLNPLVFVKAIFAKD
                     KERFNRENTQLLSNRHKLYEAVAARAQVLAFETPESLLPNLQSRLDALADSLADNNGK
                     TANTRLYARFFSLGKLAFGHAHKVDKLVGKSFPKSLVADPNYDPFANGSFDVTPYYPD
                     ALVQSWLDGKSDKRTQMLDEARKDLSQAVEAAATAIIVILRP"
     gene            complement(3531..3672)
                     /locus_tag="PKO06_08905"
                     /pseudo
     CDS             complement(3531..3672)
                     /locus_tag="PKO06_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
BASE COUNT          770 a         1051 c         1105 g          746 t
ORIGIN      
        1 catctgctgg cgagttgcat ctatcatcgc ctgccctgtg tcacgagtct gggcgcatcc
       61 ggcaaggtcg acccgacaaa agtccgagcg accgatctgc gcaagacgca tgacgacccg
      121 ttggcgaaga ccattcgcaa gtatctctgg cgggtctacg agatcaatct gaaaagggtc
      181 tcgcagctga ccgcggtgtt ttccgatgaa gagccgatgc ggcctgaccc ggaatacacc
      241 ggctcgccgt gcggcggcgc cgagtgcgtg tgcccgaaca gtaccaatca gcatcatacc
      301 tgttcggccc gcaacatcat ctggggaagt tcggtgctgg tgacctcgat gttcggcatg
      361 accgccgcgt cgctggcggt gcgctacctg gccggagacg agagcatcga cctgaagccg
      421 gacgtgaaga ccctccccgg cgacgattcg cttgtcgatc cggccagtcg ctgggaagac
      481 ggcgtgttga aaacgtcgcc agcgagccag cctgctcatg agacggccga agatgactga
      541 cgcccatacc gatgtgtcga tgaagatcga accggcgaac gatgctcgag tgtctcctga
      601 cgacgttgtc tggagactgc gggagcgggt gaaggaactg accctgctgc atcagacatc
      661 gcgtcttctg caaagtgaga aagaccgcgt tgacgcagtc atttggcttc aagcgcctcg
      721 acacggatcg caagcacaat aacggtctgc tgttcggcct cgataccgtc atcattaagg
      781 atgccaagcg cccggttacc tttctcgccg actacttcgg tggcccggcg gcaaccgttg
      841 gcctcggtct cacgcagaac ttcaccccga agctgaattg ggcggtttcc tactatcttc
      901 cgacaaagtc caagctgccc gtgtcgcaaa ctcagctgcc caagcagctt tgggtcgctt
      961 tgacgtatta tcacagtctc tgacgacctc gccggaatgt gaggttccgg gcaccccctc
     1021 cttgcaaaca gggagggggt gctgtttttc gcctgaataa ctcggaagcc aaccactcat
     1081 acgatcagga aaacagtttc tggggcgtga gttgacgatg gaaccccctt caattttccg
     1141 gattgtggta tgctgagggt acgggtgtca gaagaaagaa ttggtgactc ggctcgaaac
     1201 tgaggacgga ggaagagatg aaagcaacgg attctctcgc tcgattgatc tgggttctgg
     1261 tgattctgac aggattgtcg gcagattgtc tgccggcctg ggattcccac ttcctgctga
     1321 ctcggatggc cacacaggcg cttggtcacc aagtgccgct gcttacgcag gaggtcgcgc
     1381 cgacgtcgct ggcgacgttc gtcaaaagaa acaatgccgc tttgaagaat acgctgcggg
     1441 cctttcatga ctggaaacag aaaacgtaca cctaccccca ctggaagttg tcagaagcga
     1501 acgccgctct tctgcagggt tctgcgatca tgacaccggt acagtttctg gaaattctcg
     1561 gcgtatcgtc gacggctttc tcgaagatgg tgtttcagaa agaatacgtt gatttgtacg
     1621 ggccaagata tgcgaatccg caccttccgc gcagttttgg cgagtggttg actctgtttt
     1681 ctgacgagcc ggactggggc atggaccagg gcttgtttgg cacaggtgac ccgcgatacg
     1741 gagatatccc ctacggagat gccgcgggaa cgggatcgca agcgccgttt cacatgacct
     1801 ttcccttcga gaactgggtg acctacaagc tcaaatcttc gctcaaacag ggcatggcac
     1861 atctgcgttt catggtgttc tgcagcctga cacaggtagc gctgaatgcc ggagatcact
     1921 tctgggcggc ccggtttctg ggaagtgccg ttcattactt tgaagacttc accatgccgt
     1981 atcatgcgag cgcggttccg tttctgaatc cgctggtttt cgtgaaagcg atcttcgcga
     2041 aggacaagga gcggttcaac cgcgaaaaca ctcaactgct ctcgaatcga cataaactgt
     2101 atgaagccgt ggccgcacgc gcgcaggtgc ttgcctttga aactcccgag agcctgctgc
     2161 cgaatctgca gagccgtctc gacgcgctgg ccgacagtct cgcggacaac aacggcaaga
     2221 ccgcgaacac gcggctgtat gcgcgattct tctcgctcgg caagctcgcg ttcggtcatg
     2281 cgcacaaggt cgacaaactg gtcggaaagt cctttcccaa gagtctggtt gccgatccta
     2341 actacgatcc gtttgcgaac ggttcgttcg atgtcactcc ctactatcct gatgccctgg
     2401 tccagagctg gctggatggc aaatcagaca aacgcacgca gatgctcgac gaagcccgaa
     2461 aagacctttc acaggcagtc gaagccgcgg cgaccgcgat tattgtcatc ctgcgaccct
     2521 gacggttctt ctcaaacaac aacgcccggc ggttaaagag acccgccggg cgttgttgtt
     2581 ttgtctctga aaaaacgctc tcagcctttg tggtcaggaa agcaaacttg tactgcgagt
     2641 tgttcgcccg cgaaggcatg tcgataccac acaaacgtgg ttgtcgacgc tccgaccgat
     2701 gtttcgtccg gcggcggtag gcagatgggc gcacctcgtg tcgaagggaa caccagcacg
     2761 agcgcatggt gccggctggt gccgcagttc gcggggcgca ggctcagttc gtaagcgccc
     2821 tctggagtga ccgggggtag tccgttgccg agatcgaacc cgatcagctc tgcttctgaa
     2881 ggcttgagaa ccgggttcag tcgaatggtg accaggactc ccgacgaagg ctcggcgatc
     2941 gtgtgacagt cgaagcgtaa caggttcccc cgtgaatcaa cgaacggaga agtcggcacg
     3001 accggaagga cgatctgttc gatcgtctga tcgcggcttt cgaggttgat atgactgagc
     3061 acggcaccgc agcgccagcc gcccgcgggc tcgagaaccc acacggtggt gcggccgaag
     3121 aagccatggg taaaggcacg ggggtgcagc gagtcgatgg caacccgtcg agccagcggg
     3181 agcgcggccg gtagccaggg ccagtctgca ccctctgtca gcaagccgct gacatcctgc
     3241 cgttcgagtt tctgcgtcgc ctcggcgcgc gacagataca aggcgtttgg ctcgtgcttg
     3301 cgggccaggg ccagatagcg agcggcctgc gcgtcagcgg cctcttcgtc atacagccgc
     3361 gagaggacaa agtatgcgaa ccccttcagg gcttcggagt tggccaggcc gacggcggtg
     3421 cgcagatcgt tcacggcttt ctggaactcg ccgcgaaaga tgtgtagacg cgcgcgaaac
     3481 aacagaatga tggcgttcgc ggggtcgagc gctgacgtct cgcccagatg gcgcccggca
     3541 tcaaccgcat cggcgccacc acgccacacg tgtcgaaata gggtttcggt gacgtaacgg
     3601 ttgggtgttc cctgtttgct gctcgcaatg gccagccgca acagcgacag cgggttgaaa
     3661 ggatgggccg cc
//
LOCUS       DAORHD010000368         3659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27941_length_3659_cov_3.033019, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000368 DAORHD010000000
VERSION     DAORHD010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27941_length_3659_cov_3.033019"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="PKO06_08910"
     CDS             <1..2074
                     /locus_tag="PKO06_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV69803.1"
                     /translation="EGFETIGIMPLRRGKRTFGVLQFNWRRTAAITWEKFLFLERVAE
                     NAARSLVLQERHEHLQERLHAHHCAEESQGEVVLRLTPDGTMAYVNPAFCEAVGVARS
                     ALLGRSLVDLCIPADRRKLGVVLLGLPPHQPSRGIEIRVPVNGCIRWLQLQFTGLYRE
                     SQSLVEVRVQGRDMTMRRRVEKGLRETLQMLGGVFRRSPLPILALDQNGLVMLWNPTA
                     EKIFGYTAEEQVGTVLPVSDTAGDAGFLREWQAMIRDGAARTLSIQLHTCDGRDMDVI
                     MSTGILTDEKGNPAGGVCICFDYDEVSRPRIRREKWESQLVRIQRMEALGRLAGGIAH
                     DFNSQLTTIMGYADMLLEELPAESSSRKWVGEILSATRRSAALTERMLAYSRAHQLKP
                     ELFDWNRLLSDLEKTFLRTLTARIELQIQTSANPALVFSDLAQCEQTLMHLVLNAADA
                     MPLHGKLLIDVKNETVFLPRPCVHGTIPVGTYVMVAVEDNGVGMDAAVQARIFEPFFT
                     TKAVGKGSGLGLSMVLGFIQQSGGFITLRSACDTGTRIELYFPAVASDQIERLRDVRV
                     SATESIDTILIAIRDNDERDRLLQRVRGLGYRALSVSRGDLVLDFCRRFGESIHLIVL
                     DAELIEAPGITEFRQTVLREHPEARVLLLTADEAGVAQAWAGHPARQLLQRPATEGLI
                     IARIEDLL"
     gene            2278..>3659
                     /locus_tag="PKO06_08915"
     CDS             2278..>3659
                     /locus_tag="PKO06_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV69804.1"
                     /translation="MKDTLLKIVEAARALGADYADVRQITTEIETLAVKNGQVIGIVK
                     NVSEGYGVRVLKKGAWGFSCSPDLPLWERVVREAVAIADCSALAPRDGGVKFPAAKAI
                     VAEYKTPFAVDPFTVSIEKKLELLMKADRAMREVPEIKVALSSLGFQRETRLFASTEG
                     SFIRQELLESGGGIECTAVGSGEVQKRSYPNSFGRNQLTSGFEMVEAMDLPGNARRIA
                     DEAAALLTAPACPSGTKTIILDATQLALQIHESCGHAVELDRVYGMEASFAGSSFLTT
                     EKLNTFQYGSDIVNLYQDGTVKTGLGTYGYDDDGVPTCRTDLVKNGRFVGYLSNRETA
                     ADLGVPSCGANRATSWRFTPIVRMTNINLAPGTWDFDALISDTREGLYLITNKSWSID
                     DKRMNFQFGTELAYEIKDGKLGRLLKNPIYTGMTPEFWKNCDAICNEKHWQILGTPNC
                     GKGQPMQTAHV"
BASE COUNT          739 a         1002 c         1160 g          758 t
ORIGIN      
        1 ggaaggcttt gaaacgatcg gcatcatgcc gttgcgacgc ggcaagcgaa ccttcggtgt
       61 gctgcagttc aactggcgtc gcacggcggc catcacctgg gaaaagtttc tgttcctcga
      121 gcgggtggcc gagaatgccg cgcgatcgct ggttctgcag gaacgacatg agcatctgca
      181 ggaacgtctg catgctcacc attgcgccga agaatctcag ggcgaggttg tgctgcgact
      241 tactcccgac ggcacgatgg cgtatgtgaa ccccgcgttc tgcgaagcgg tcggcgtggc
      301 cagatcggcg ttgctgggca gatcgctggt cgacctgtgt attcctgccg atcggcgcaa
      361 actgggcgtg gtgctgctgg ggttgccacc gcatcagccg agtcgcggca tcgagattcg
      421 cgtaccggtg aatggttgca ttcgctggct tcagttgcag tttaccggcc tctatcgcga
      481 atcgcagtcg ctggtcgagg tgcgcgtgca ggggcgagac atgacgatgc ggcgacgcgt
      541 ggaaaaggga ctgcgtgaaa cgctgcagat gctgggtggg gtctttcgtc gttcgccact
      601 gccgattctg gcactcgatc agaacgggct ggtcatgctg tggaacccga ccgcggagaa
      661 gatcttcgga tacactgccg aggaacaggt gggcacggta ctgcccgtga gcgacaccgc
      721 cggtgacgcc gggtttctgc gggagtggca ggccatgatt cgtgacggag cggctcgcac
      781 tttgtcgatc cagctgcaca cgtgcgacgg acgagacatg gatgtcatca tgtcgaccgg
      841 gatcctgacc gacgaaaaag gcaatcccgc cgggggagtc tgtatctgtt tcgattatga
      901 tgaagtctcg cgacctcgaa tcagacgcga aaagtgggaa agccaactgg tacgtattca
      961 gcgcatggaa gcgcttggcc gccttgccgg tgggattgcg cacgacttca acagtcagtt
     1021 gacgaccatc atgggctatg ccgacatgct gctcgaagag ctgccggccg agagttcgtc
     1081 acgcaaatgg gtcggagaga ttctgtcagc gacccggcgt tccgcggccc tgaccgaacg
     1141 catgctggct tatagtcggg cccatcagct gaagcccgaa ctcttcgact ggaatcgtct
     1201 gctttccgat ctcgagaaga cgtttcttcg caccctgaca gcgcgtatcg agctccagat
     1261 ccagacctcc gcgaatccgg cgctggtctt ttctgatctg gctcagtgcg aacagacgct
     1321 gatgcatctg gttctgaacg ccgcggatgc catgccatta cacggaaagc tgttgatcga
     1381 cgtgaagaac gagacggtgt ttctgccccg cccgtgcgtg cacggaacga ttccggtcgg
     1441 gacatatgtg atggttgcgg tcgaagataa cggggtcggc atggatgcgg cggtacaggc
     1501 aaggatcttc gagccgtttt tcacgaccaa ggcggttggc aaaggaagcg gactcggact
     1561 ttcgatggtg ctggggttca tccaacagag cggcgggttc attactctgc gcagtgcctg
     1621 cgacacggga acccgcattg aactatactt tcccgcggtt gcttcagatc agatcgagcg
     1681 cttgcgcgat gtgcgcgttt ccgccaccga gtcaatcgac acgattctga ttgccattcg
     1741 cgataacgac gagcgcgatc gattattgca gcgtgtacgt gggctggggt accgcgccct
     1801 ctcggtctcg cgcggagacc tggttctgga tttctgtcgc cggttcgggg aatccattca
     1861 cctgatcgtt ctcgacgcgg agttgatcga ggcacccggc atcaccgagt tccggcagac
     1921 ggtgctgcgc gaacatccag aggctcgagt gctgctgctg acggctgatg aagcaggtgt
     1981 cgcgcaggcg tgggccgggc acccagcccg gcaacttctg cagcgaccag caactgaggg
     2041 cctgatcatc gcccgcatcg aagacctgct gtaagaacga tctttcgctg tttccgttca
     2101 tcgaaaagct cggtcgtcga ctcacgatgg gcacgaaccg gggtttgccg ccgttgtcgg
     2161 cgagttggtt tccttgtcga aagagctgct gcgaggcatt atcttgtgct cgacggaacg
     2221 agggaaatcg gctagaatct ggccaaactt gccggtattc atgggaaagg aagcatcatg
     2281 aaagatacgc tgttgaagat cgtcgaggcg gcgcgcgcac tgggtgcgga ttacgccgac
     2341 gttcgccaga tcacgaccga gatcgagaca ctggcagtga aaaacggtca ggtgatcggt
     2401 atcgtgaaga acgtcagtga agggtacggg gtgcgggtgt tgaaaaaggg cgcctggggc
     2461 ttcagttgca gccctgacct cccactctgg gaaagagtcg tgcgtgaggc ggtggcgatt
     2521 gcggattgtt ccgctcttgc ccctcgtgat ggcggtgtga agtttccggc tgccaaggcg
     2581 attgtcgccg agtacaagac tccctttgcc gttgatccat tcacagtgtc gatcgagaaa
     2641 aagctcgaac tgctgatgaa ggcggacaga gcgatgcgcg aggttcctga gatcaaagtc
     2701 gcgctgtcgt cgctcggctt tcagcgtgaa actcgtctgt tcgccagcac cgagggatcg
     2761 ttcatccgcc aggaactgct ggaatcgggc ggtggcatcg aatgtaccgc tgtcggcagc
     2821 ggggaagtgc agaagcgcag ttatcccaac tccttcggtc gtaatcagct gaccagtggc
     2881 tttgagatgg tcgaagccat ggatctgccc gggaacgccc gccggattgc cgatgaagcc
     2941 gccgcgttgc tgacggctcc agcgtgcccg agtggcacga agacgatcat tcttgatgcg
     3001 acgcagctgg ccctgcagat tcacgagtcg tgcggtcacg ccgttgagct cgaccgggtc
     3061 tacggcatgg aagccagttt tgccggatcc tcgtttttga ccaccgagaa gctcaatacc
     3121 ttccaatacg gttccgacat cgtgaatctg taccaggatg gaaccgtgaa gactggcctg
     3181 ggaacctacg gatacgatga tgatggcgtg ccgacctgtc gcaccgatct ggtgaagaat
     3241 ggccgcttcg tcggctatct gtcgaatcgt gagacggcag ccgatctcgg agttcccagc
     3301 tgcggagcga atcgcgccac ctcctggcgg ttcaccccga tcgtccgcat gacgaacatc
     3361 aatctcgcgc ccggaacgtg ggatttcgac gctctgatca gcgacacgcg cgagggtctg
     3421 tatcttatca ccaacaagtc gtggtcgatc gacgacaagc gcatgaactt tcagtttggc
     3481 actgaactgg cctacgagat caaggacggc aagctgggtc gactgttgaa gaacccgatc
     3541 tacactggaa tgacccccga attctggaag aactgcgatg caatctgcaa tgaaaaacac
     3601 tggcagattc tgggcacccc gaactgcggc aaggggcagc cgatgcagac ggcgcacgt
//
LOCUS       DAORHD010000369         3655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_27982_length_3655_cov_3.333889, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000369 DAORHD010000000
VERSION     DAORHD010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27982_length_3655_cov_3.333889"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="PKO06_08920"
     CDS             <1..1362
                     /locus_tag="PKO06_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HNV69805.1"
                     /translation="RDAVRQSVTRTQRPVIPTLASPIRQSDAQRRLALIVDDLESRQS
                     PNPPAAASAADYPGTPLPQSRRVTREIEIDLSIPGWASTGLWAMAGEAVDVTLPASCS
                     GKGFRLLIGCHTDTLWHTTEWLRHPDISRSFPAKSGLNRISNPHGGLIYLDIPRAVEG
                     SSPGNVRVQISGNAVEAPWYRLGKTTNDEWQRMRQAPAPWAELQCERIILTVPSEVVR
                     SLADPRPLMEFWHNALGWYVELGVRPLDRRPQRIVADRQISCGWLHAGYPIMANIAVA
                     PKMVNLAGLAHPEKDSEGAWGFWHELGHNHQRPEWTFSGATEVTCNLYSLYVEEKLRG
                     NPPRHHPWLRSTAEKVRSYLAHPDFELWKKDPGLGLVFFVELQSAFGWEAFRKVFASY
                     AKTPEHELPKTDDLKRDQWLIRFSQAVGRNLTSHFNKWGIPVSPTAIRAVSHLPKWEA
                     ERR"
     gene            1417..1842
                     /locus_tag="PKO06_08925"
     CDS             1417..1842
                     /locus_tag="PKO06_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69806.1"
                     /translation="MLKTLIDRLKKLLFRADLAPETLRQVGKDIVEGGLTRRSFSNDY
                     FDLFIWLDDQGQLREFQLCYEKMGNEHVITWSHEGTLSHRKVDGGKQQRKMSSIFVPD
                     GAFPRTLVCQRFAQNSSQLDPHLVAQIIEKLESYSGPQQ"
     gene            1905..2579
                     /locus_tag="PKO06_08930"
     CDS             1905..2579
                     /locus_tag="PKO06_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69807.1"
                     /translation="MSRLIVILTLCLFPSILMAQYTNYSTGNTFNNPGSALIDTFIQS
                     KMNQQMMLKSMNKKGKNTPETVKNTPHSTNFDYSSSTFKGMEKRLLPDIIASAVADVS
                     AADRLLLSSRLAEAIENYEVQSGRKNNVSHAISFLLGVSLQITRHIEVSDADLEELAQ
                     AVHGKIQADADWKNLGDEQKQLLYEETLIQASLLLALQEKGDAASRQSAQDQAKQCLN
                     RYGITE"
     gene            2727..3563
                     /locus_tag="PKO06_08935"
     CDS             2727..3563
                     /locus_tag="PKO06_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69808.1"
                     /translation="MKYRMCVVLLIGLLTLSTSGNALTSETSKSPINPFYALLIAWTF
                     DVAELEALTRGYVSREATGIYSNEKYFEKDGLELRLLGMAHVADKNFYEDIKNSLRGK
                     PGLILMEGVTDEKDLLETPLDLSSVAEKLGVDDQRKTFTPKSMPAEVTVIRADVDVSD
                     FASDTIELLNLVGKVYSKKGFNFGHFLMMYLKLSQPEVTRTFFTDLIEKRNACLIGHV
                     QNNLKKYPRIVIPWGAMHLPDIERWVSDNGFTLKSQKGRLLLRFPNYLQYILPEKPRK
                     QG"
BASE COUNT          894 a         1022 c          911 g          828 t
ORIGIN      
        1 cgcgatgccg ttcggcaaag tgtcactcga acgcaacggc ctgtcattcc aacgctcgcc
       61 agtcccattc gccaatcaga tgcacaacgt cgcctcgccc tcatcgtcga cgatctcgaa
      121 tcccgtcagt ccccaaaccc acctgcagcg gcctcggcgg ctgactatcc cggaacaccg
      181 ttgccgcagt ctcgtcgagt cacccgcgaa atcgagattg atctctcgat tccggggtgg
      241 gccagcacgg gcctctgggc gatggccggc gaagcggtcg acgtgactct ccccgcctcc
      301 tgttcaggca agggattccg tctgctcatc ggctgtcata ccgataccct gtggcacaca
      361 accgagtggc tcaggcatcc cgacatctcg cgaagctttc ctgccaaatc cggattgaac
      421 aggatttcca atccgcatgg aggcctgatc tatcttgaca ttcctcgcgc agtcgaaggt
      481 tcaagccctg gaaatgtccg ggttcaaatc tctggaaatg ccgttgaagc cccttggtac
      541 agacttggga agacaacaaa cgacgagtgg cagcgcatgc gacaagcacc ggctccctgg
      601 gccgagttgc agtgcgagcg aatcatcctg accgtccctt ctgaagtggt tcgctctctg
      661 gcggatccac gaccactgat ggagttctgg cacaacgcgc tcggctggta cgtcgagtta
      721 ggcgttcgcc ctctcgaccg gagaccgcag cgcatcgtcg ccgatcggca gatttcctgc
      781 gggtggcttc atgccgggta tccgatcatg gcgaacattg ccgtcgcccc caagatggtc
      841 aatctcgccg gacttgctca tcctgaaaaa gacagtgaag gagcctgggg attctggcat
      901 gaactgggac acaatcatca acgcccggaa tggaccttca gcggcgcaac ggaagtaacc
      961 tgcaacctgt attctctgta cgttgaagaa aagctgcgcg gaaaccctcc tcgtcatcat
     1021 ccatggctga gaagcacggc cgaaaaagtc cgatcctatc tcgcccatcc cgatttcgaa
     1081 ctgtggaaga aagaccccgg gcttggcctg gtctttttcg tcgagctcca atctgccttc
     1141 ggttgggagg cattcaggaa agtcttcgcc tcctatgcga aaactcctga acacgagctg
     1201 ccgaagacgg acgatctgaa gcgtgaccag tggctgatcc ggttcagcca agccgttgga
     1261 cggaatctga cctctcactt caataagtgg ggtattcccg tttctccaac cgcaattcgg
     1321 gctgtttctc atcttcccaa atgggaggcc gagaggagat gatatacttt cgcgtgaacg
     1381 catcgttcaa aggccatcgt ctttcctggt gaatacatgt tgaaaacatt gattgatcga
     1441 ctgaaaaaac tgctgtttcg tgcggacttg gcacccgaaa cactgaggca agtcggcaaa
     1501 gacattgtcg agggaggact gacacgtcgc agtttttcca atgactactt cgatctgttt
     1561 atctggctcg acgaccaagg tcagctgcga gagtttcagc tgtgttacga gaagatgggc
     1621 aatgaacatg tcatcacttg gagtcatgag ggaactctct cgcatagaaa agtcgatggc
     1681 gggaagcaac agcgaaagat gtcctctatc ttcgtccccg acggagcttt tcccagaacc
     1741 ttggtttgcc aacgcttcgc gcaaaactct tctcaactcg acccacacct cgtcgcccag
     1801 attatcgaaa aacttgagtc ctacagcgga ccccagcaat gatcaccggc gcccgccacc
     1861 accgagcgtt ctgatccgta tttctttctg aaaggccaat ctccatgtcg cgtttgattg
     1921 tcattcttac cctctgtctc tttccatcaa tactcatggc tcagtatacg aactatagta
     1981 ccggtaacac tttcaataat cccggttctg ctctcatcga cacctttatc cagtcgaaaa
     2041 tgaaccagca gatgatgctg aaaagcatga acaagaaggg gaaaaatacc cccgaaacgg
     2101 tgaaaaacac tcctcactca acgaacttcg attactcaag ttccacattc aagggaatgg
     2161 agaagcgtct tctgcccgat atcatcgcca gtgcggtagc cgatgtttct gcagctgata
     2221 gacttctgct gtcttcccgg ttagccgaag ccatcgagaa ttacgaagtt cagagcggca
     2281 gaaagaacaa tgtttcccac gcgatcagtt ttctgctggg tgtcagtttg cagatcaccc
     2341 gacacatcga ggtgagcgac gccgacctcg aagaactggc gcaggcagta catggcaaga
     2401 ttcaggcaga tgccgattgg aagaacttgg gcgatgaaca gaaacagttg ctgtatgagg
     2461 aaactctcat tcaagcctcg ctcctgctgg cgctccagga gaaaggagat gctgcctccc
     2521 gacagtcagc gcaagatcag gcaaagcagt gtctgaaccg ttacggaatt actgagtgat
     2581 cagctcacga gtacaggatt cgagccaagt cgaactacag attactgctg caccgccttg
     2641 aattcccgat agaactgatg cgccttgcct tcgatgcgta taccttctat cagaccagac
     2701 agagacgacc ttgcaggaga ccctctatga aatacagaat gtgtgtggta ctgctcatcg
     2761 gtttgctaac attgtcgact tccggcaacg cactgacaag cgagacatcg aaaagtccga
     2821 tcaatccatt ttatgcgctt ctgatcgcct ggacatttga tgtcgcagaa ttagaagcac
     2881 ttactcgtgg ttacgtctca cgagaggcga ccggcatcta ttccaacgag aagtatttcg
     2941 aaaaagatgg tctggaactt cgtttgctgg gaatggctca cgttgccgac aagaactttt
     3001 atgaagatat caagaacagt ctgcgcggaa aaccaggcct cattctcatg gaaggcgtta
     3061 ccgatgagaa agaccttctg gagaccccgc tcgatctcag ttcagtggcc gagaaactgg
     3121 gcgtcgatga ccagcgtaag accttcactc ccaaatcgat gccagccgag gtaacggtta
     3181 tccgtgccga tgtcgacgta tcggactttg cttctgatac gatcgagctc ctcaatctgg
     3241 tcggaaaggt gtattcaaag aagggtttca acttcggtca ttttctcatg atgtatttga
     3301 agctctcaca gcccgaagtc acccggacat tcttcaccga cttgatcgaa aagcgaaatg
     3361 cctgtctgat tggccacgtg cagaacaacc tgaagaaata cccgagaatt gtcattccat
     3421 ggggtgcgat gcacctgccg gatatcgagc gctgggtcag cgataacggc tttacgctca
     3481 agtcccaaaa ggggcggctt ctcctgcgct ttccgaacta tcttcagtac attctgcccg
     3541 agaaaccacg aaaacagggc tgatcgctcc gaatctgagc gtcacctctc acttttccgt
     3601 ctcacgtcac tgcaagggga catcgaagta ttcctgtgac ggacagaatt ccagc
//
LOCUS       DAORHD010000370         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28044_length_3650_cov_2.970793, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000370 DAORHD010000000
VERSION     DAORHD010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28044_length_3650_cov_2.970793"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="PKO06_08940"
     CDS             <1..1454
                     /locus_tag="PKO06_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69809.1"
                     /translation="EVVVRMPATMPWVLETSQWPEKNSERLTLTDTVENGQRTIRAVF
                     HDREGYIPEQNMLPRYRIFPRLLMSPQHSWETIAQELDKGLASVAPSPELLAAFVQES
                     GAMKVSGDFEKARTLYDYLLRRIQMVGVGLGDYGRYVPPSIDGVLTKKYASAFARVAL
                     MHFALKKLGIASAIGFVDAWQDGGLKERIPSLGQAADALLRVSLDGRTLYLTLDSDYL
                     SFGVLHTGYQGARAGFLINGRIVFETLPEGLEWNRADQQVFVKVLADGSFEVKDLRNF
                     RGIHEPGLRGLRSAKEKKKLQWAQGLVKRIHPKAELIDYSLTDVNDLTAPLSLALTYR
                     IPDAAIKAGENLLAFKNLWVRYGAGSASLEKRKYPMEYYAWSDSANNIVFELPDGFRW
                     VPWGRTFNYACGFLSYASNLMQNGRMVSFSDRLTVQRKELDVIDAYPHYRGCLTRMAD
                     LSNQWMIIERVAVATPSAASATVNPASGVTPTK"
     gene            1465..3465
                     /locus_tag="PKO06_08945"
     CDS             1465..3465
                     /locus_tag="PKO06_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV69810.1"
                     /translation="MIDALLAQWLYVWPVLLVLFGPFGYWLSEPLTTAFVQVVAAIVF
                     VLVGFSCFYHQGTKTSAWFFRLCLLWLIGWGISVYASADPSVSAPVYWKLLGLVLVAG
                     SMRYALCEEGVQSAFLHAAALAVLVHGCAGVFEYFDPQPMPLSWIDPSQRGVIPFRSS
                     GFFADPNVYGAYLAALLPLVLAGWFSTTLTPATAVLWPAAFLAGSAGLLVSYSRGAWL
                     GALAGLAVVVILRKPGWSLTWRRKVLLAVSLVIVAVFLAGPFKYRFFSMARPNDMTIA
                     QRALLTQGMWDMARHAPWQGFGLHTFSQIYPQFRRVGGDYPMYAHNEFLQTFLEGGFI
                     SLLGLVGMVLTLLSLLWRLSRRDAGHVSWLASACGGTFVCVLVHNLSGFSARIFPVSL
                     VLAIAVGGLLRAMRREDLAPPLMIPRGLGRIVGGTFLALYLLFSFKTLHVQVLLQDAS
                     AALSAKEYLVAESSLQAILKIDRQVPQAHYLLALIQEGYGKWNVAEHHLDTAMKLNPS
                     EAIYWKERSRLETKMGGARAEEYLKRAVALDPASEHFRLEYARQLASAGRLLEARDQL
                     DAALRTSPGWHEVYVGYQSIEHLRRQLEERLQMQKPPSGSPPVGGATPVHGVSGQVGI
                     ELGVRMASETVTNASQPGLASETSFRPGPASAGQPVEKREPN"
     gene            3485..>3650
                     /locus_tag="PKO06_08950"
     CDS             3485..>3650
                     /locus_tag="PKO06_08950"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNV69811.1"
                     /translation="MNILILNGPNLNMLGVREPEVYGRESWSDVEVRLLALAKSLGVQ
                     LTMRQSNHEGV"
BASE COUNT          642 a         1005 c         1171 g          832 t
ORIGIN      
        1 acgaagtggt cgtgcggatg ccggctacga tgccctgggt tctcgagact tcgcagtggc
       61 ccgagaagaa ctcggaacga ctgacgctga ccgataccgt cgaaaacggc cagcggacca
      121 ttcgggcagt gtttcatgac cgcgagggct acattcctga acagaacatg ctgccgcgct
      181 atcgtatttt tccgcgcctg ctgatgtccc cacagcattc gtgggaaacc atcgctcagg
      241 aactggacaa gggactggcg tcggtcgcac catctcctga acttctggcg gcgttcgtgc
      301 aggaatccgg cgcgatgaag gtttctggcg actttgagaa agcacgcaca ctgtatgatt
      361 acctgctgcg tcgcattcag atggtgggcg tcggcttggg cgactatggg cgttatgttc
      421 cgccgtcgat cgatggggtg ctgaccaaga agtatgcgag tgcctttgcc cgggtggcgt
      481 tgatgcattt cgcgctgaaa aagctgggca ttgcttccgc catcggtttt gtcgacgcct
      541 ggcaggacgg aggtctcaaa gaacgcatcc ccagtctggg gcaggctgct gacgcgcttc
      601 tgcgcgtgtc gctcgatggt cgcaccctgt atctgaccct cgattctgac tatctgtcgt
      661 tcggtgttct gcacacaggg tatcagggtg ctcgcgcggg ctttctgatc aacggccgca
      721 tcgtgttcga aaccttgcct gagggtctcg agtggaatcg cgcggatcag caggtgttcg
      781 taaaagtgct cgccgacggc agcttcgagg tgaaagatct gcgcaacttc cggggcattc
      841 atgaacccgg cttgcgcggc ctgcgttccg ccaaggagaa gaaaaagctg caatgggcgc
      901 agggcttggt gaagcgcatt caccccaaag ccgaactgat cgattattcg ttgaccgatg
      961 tcaatgacct gaccgcgcct ctcagtctgg ctttgacgta tcgcattccc gacgcggcga
     1021 tcaaggcggg cgagaacctg ctggcgttca agaatctgtg ggtacgctat ggggctggct
     1081 cggccagtct cgaaaagcgc aaatacccga tggaatacta tgcgtggtcg gactcggcga
     1141 acaacattgt gttcgagctt cccgacggtt ttcgctgggt tccgtggggc agaacgttca
     1201 actacgcctg cggttttctg agctacgcca gtaatctgat gcagaatggg cgtatggtca
     1261 gcttcagtga tcgtctgacg gtgcagcgca aagagttgga tgtgatcgac gcttatccgc
     1321 actatcgagg gtgtctcacc cgtatggccg atctctcgaa tcagtggatg atcatcgagc
     1381 gtgtcgcagt ggcaaccccc tcagcggcga gcgctaccgt gaaccccgcg tctggagtga
     1441 ccccgacaaa atgaacgtcg atctatgata gacgctctgt tggctcaatg gctttatgtg
     1501 tggccggtac tcctggttct tttcggacca ttcggatact ggctgagtga gccgttgacg
     1561 acggcgtttg tccaggttgt cgcggcaatt gtattcgttc tggtgggctt tagctgcttc
     1621 tatcaccagg gaaccaagac gtcggcgtgg ttcttcaggc tgtgtctgct ctggttgatc
     1681 ggctggggca tctcggtgta tgcctcggcc gatccgtccg tctcggcccc cgtctactgg
     1741 aaactcctcg gtctggtgct ggtcgctggt tcgatgcgct acgccctgtg cgaagagggg
     1801 gtgcagagcg cgtttctgca tgcggctgcc ctggccgttc tggtgcacgg gtgcgcgggg
     1861 gtgttcgaat acttcgaccc gcagccgatg ccgctctcat ggatcgaccc ttcgcagcgg
     1921 ggtgtgattc cttttcgcag ttcgggattc ttcgctgacc ccaacgtcta tggggcatat
     1981 ctggctgcgc tcctgccgct ggttctggca gggtggttct cgaccacgct gacaccagcg
     2041 accgcggtgc tgtggcccgc ggcgtttctg gccggcagtg ccggtctgct ggtgagttac
     2101 agccggggag cgtggctcgg agcactggcc gggttggccg tggtggtgat tctgcgcaag
     2161 cccgggtggt cgttgacctg gcgacgcaaa gtgttgctcg cggtcagtct ggtgattgtg
     2221 gccgtgttcc tcgcggggcc gttcaaatat cgcttcttca gcatggctcg cccgaatgac
     2281 atgacgattg cccagcgggc actgctgacg cagggaatgt gggatatggc ccgccacgcg
     2341 ccgtggcagg gatttgggct gcacaccttc agtcagatct atccgcagtt tcgccgggtg
     2401 ggcggtgact acccgatgta cgcgcacaac gagtttctgc aaacctttct cgaaggtggg
     2461 ttcatctcac tgctgggatt ggtcggcatg gtcttgaccc tgttgtcgct gctctggcgg
     2521 ctttcgcgcc gggacgcggg ccatgtctcg tggctggcaa gcgcctgcgg tggaacgttt
     2581 gtctgcgtgc tggttcacaa cctcagcggc ttttcggccc gcatttttcc ggtttctctg
     2641 gtgctggcca ttgcggttgg cgggttgctg agagccatgc gtcgcgaaga tctggcaccg
     2701 ccactgatga ttccgcgcgg tcttgggcgc atcgtcggtg ggacatttct ggcgttgtat
     2761 ctgctattct cgttcaagac tctgcatgtg caggtgttgt tgcaggatgc gtcagccgcg
     2821 ctgagtgcca aggagtatct ggtggcggaa agctctcttc aggctattct caagatcgat
     2881 cgacaggtac cgcaggctca ctatctgctg gccctgattc aggaaggata cggcaagtgg
     2941 aacgtcgctg agcatcatct cgacaccgcg atgaagctga acccgagcga ggccatctac
     3001 tggaaagagc gcagtcgact cgagacgaag atgggagggg cgcgggccga agaatacctg
     3061 aagcgagcgg ttgcactcga ccccgcttct gaacactttc ggcttgagta tgcccggcaa
     3121 ctggcatctg ccgggcgttt gctcgaagcc cgcgatcaac tcgatgctgc gctgcggact
     3181 tccccggggt ggcatgaggt ctacgtcggg tatcagagca tcgaacacct gcgtcgtcag
     3241 ctggaagaac ggctgcagat gcagaaacct ccgagtggct ctccgcctgt cgggggagcg
     3301 actcccgtgc acggcgtctc gggccaggtt ggcatcgagc tgggagtgcg catggcaagc
     3361 gagacggtca cgaatgcgtc acaaccaggg ctggccagtg aaacctcttt ccgaccgggg
     3421 ccggcatctg cgggacaacc ggtcgagaag cgagaaccca attgattctg agtcgaggaa
     3481 gatcatgaac attctgattc tgaacggacc taatctgaac atgctcggcg tgcgcgaacc
     3541 cgaagtctac ggtcgcgaga gctggtctga cgtcgaggtg cgcctgctgg ccctggcgaa
     3601 atcactcgga gtgcagctga ccatgcgaca gtcgaatcat gagggggttc
//
LOCUS       DAORHD010000371         3647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28083_length_3647_cov_2.746381, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000371 DAORHD010000000
VERSION     DAORHD010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28083_length_3647_cov_2.746381"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKO06_08955"
     CDS             complement(<1..670)
                     /locus_tag="PKO06_08955"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV69812.1"
                     /translation="MISRLLHCVFLGFACALLAGCGGFPVSPEGNAILVPSPNHNARR
                     PNFVIIHDTGSGDVERALRTLTDPAREVSSHYLIGRDGTLYQLVDENRRAWHAGASYW
                     GGNSDINSASIGIELDNTGAEPYSEAQILTLLDLLWGLKTRYRIPSSNFLGHGDVAPG
                     RKVDPSRYFPWQRLATEGFGLWCRQQATTLLPDLTDPVLALQAIGYDVANPEAALAAF
                     RRHFL"
     gene            complement(763..2016)
                     /locus_tag="PKO06_08960"
     CDS             complement(763..2016)
                     /locus_tag="PKO06_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015765589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV69813.1"
                     /translation="MSESEIWITLSLMVLCLIAEGFFSGSELGVVSADRMKLRHDAAK
                     GSRGARLALEMLEKRPEWLLSTTLVGTNIAVVTNSTIATALMISLFGEAGSWLAVILV
                     VPLAWVFGEIVPKSVFQQRADTITPYVIYVLRFFSILFWPILVVFVTLSKFLSRLAGS
                     RDEHNPFTLREQIQSMVQMPPQEGGDIQPIEKTMIRRMFNFSETTVYNVMVPLIDVNA
                     VEKHVTVGEAVRLAVESSHIRLPVYDGRIDRVVGVLNTMDLLGMDESSSIEPFIRPTR
                     YVPASKSAESMLVELRKDGDAMAVVMDEFGGAEGIVTIEDIIEDVVEDMQDEYDRQEK
                     PAEWLKKLGHHDYLVSARADPGMLVEKLGLKLPGNGNYDTLSGFLLEHAREIPRPGTT
                     LEVEGIKFTIQRATPQVIQEVQVRW"
     gene            complement(2009..3409)
                     /locus_tag="PKO06_08965"
     CDS             complement(2009..3409)
                     /locus_tag="PKO06_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV69814.1"
                     /translation="MDPALWIELVVFIVLLGFSGFFSSTETSLFSLSTFQLEQMRAAK
                     NPRIDLIERLRSEPRRLIVTILIGNEFVNVSASVISAAMIIHLFGAENEMINLLVMVP
                     ILLLFGEITPKVLAIRNNVAFANVECRPIALVARLITPLREVIRYISDFFITLIVGKQ
                     RSQGNLVTEDMIRTLVHDAVGEGALDSQEAQYIEKIFEFGNKSLRDIMRPRSDIQFLS
                     ADLPVAGLIAQIKASRQSRYPVFKGHRDTILGILHTRDLLGVDLARLERDPQGFRKLL
                     RQPNFFPESKPAVELFHDFRQRKLSFGLIVDEYGGVTGLVTMEDLLECVFGEIVSPSD
                     SEVVDQHLLSELPDGRRLIETSILLDDFNREFSVGIASAEVETLAGALLAAFGELPAA
                     GAATVLCGLKFTVDNIEHNRITRVVVERLPISVEPQETECADTGSASPELLPATVPAA
                     QEDVAAPRPNGGRQNV"
BASE COUNT          760 a         1064 c         1100 g          723 t
ORIGIN      
        1 ccaggaaatg ccgacgaaac gccgccaagg cggcctcagg gtttgccacg tcgtatccga
       61 tggcctggag tgcgaggaca ggatcggtta gatccggaag cagcgtagtc gcttgctggc
      121 ggcaccacaa accaaatccc tcagtcgcca gacgttgcca cgggaagtac cgactcgggt
      181 cgacttttcg accaggagcc acatcgccgt gccccaggaa attgcttgac ggaatccggt
      241 aacgggtttt caggccccac agcaaatcaa ggagagtcaa gatctgcgcc tcggagtaag
      301 gttcggcccc cgtattgtcg agttcgatac cgatcgacgc ggagttgata tcgctgttgc
      361 ctccccagta ggacgctccg gcgtgccagg cgcgcctgtt ttcatcgacc agttggtaca
      421 gggtgccgtc gcgtccgatc agataatggg aactgacttc gcgggcggga tcggtgagcg
      481 tcctgagtgc acgctcgacg tcgccactgc cggtgtcgtg aatgatcacg aagtttggcc
      541 ggcgggcgtt gtggtttggc gatggcacaa gaatggcatt tccctcggga ctcaccggaa
      601 atccgccgca tccggcaagc aatgcacagg cgaaaccaag aaacacgcaa tgcaggagcc
      661 gagaaatcat ctcaagtcac cagggttgaa atcttcatcc gcggcaaccg gcactcctgc
      721 cgtcgagggc gcgccaagcc aaacgaactg tagcagcaaa gattaccagc gaacctgcac
      781 ttcctggatt acctgtggcg tggcgcgctg gatcgtgaac ttgatgcctt caacctcaag
      841 cgttgttccc ggacggggaa tctcgcgtgc gtgctccagg agaaagccgg agagtgtgtc
      901 gtagtttcca ttgccgggga gcttcagtcc caacttctcg accagcatac ccggatcggc
      961 gcgggcactg accaggtagt catggtggcc cagtttcttc agccactcgg ccggcttctc
     1021 ctgccgatca tattcgtcct gcatgtcctc gaccacgtct tcgatgatgt cctcaatggt
     1081 gacgatgcct tccgcgccac caaactcgtc catcaccacc gccatcgcat caccatcctt
     1141 gcgcagttcg acgagcatcg attcggcact cttgctggcg ggaacgtagc gtgtcgggcg
     1201 gatgaatggc tctatcgacg aggactcgtc cataccgagc aggtccatgg tgttcaatac
     1261 gccgacgacc cggtcgatac gcccgtcgta aaccggcagg cgaatgtgcg aactctccac
     1321 cgccagacgc accgcctcgc cgacggtcac gtgcttttcg acggcgttca cgtcgatcag
     1381 gggcaccatg acgttgtaaa ccgtggtctc ggagaaattg aacatgcgcc ggatcatcgt
     1441 tttctcgatc ggctggatgt ctccgccttc ctgcggcggc atctgcacca tgctctggat
     1501 ctgctcgcgc agagtgaagg ggttgtgttc gtctcggcta ccggcaagac gtgagagaaa
     1561 cttcgatagc gtcacgaaga cgaccagaat cggccagaag aggatcgaga aaaagcgcag
     1621 cacgtagatc acgtacggcg tgatcgtgtc ggcgcgctgc tggaagacgc ttttcgggac
     1681 gatttcaccg aacacccagg ccagcggcac gaccaggatc accgccagcc agcttcccgc
     1741 ctcaccgaac agcgagatca tcagagccgt ggcgatcgtc gagttggtga ccaccgcaat
     1801 gttggtcccc accagggtcg tcgagagcag ccactccggc cgcttttcga gcatttccag
     1861 ggccaggcga gcaccgcgcg aacccttggc ggcatcgtga cgcaatttca tgcggtcggc
     1921 actgacgaca ccaagctccg agcccgaaaa gaatccctcg gcgatcaggc agagcaccat
     1981 caacgagagc gtgatccaga tttcagattc agacattctg acgtcccccg tttggtcgcg
     2041 gcgcagccac gtcctcctgg gcagccggca cggtggctgg cagcagttcg ggggacgcac
     2101 tgccggtgtc ggcgcactcc gtttcctgcg gttcgaccga aatcggcagg cgttcgacga
     2161 cgacgcgcgt gatgcggttg tgctcgatgt tgtcaaccgt gaatttcaga ccacagagga
     2221 cggttgctgc gccggcagcg ggcagctcgc caaaggctgc aagtaaagcg ccggccagag
     2281 tctcgacctc tgcgcttgcg ataccgaccg agaactcccg gttgaagtcg tcaagcaaga
     2341 tgctggtttc gatcaaacga cgcccgtcgg gaagttcgct gagcaggtgt tggtcaacca
     2401 cctcgctgtc cgaggggctg acgatctcgc cgaaaacgca ctcgagcagg tcttccatgg
     2461 tcaccaggcc ggtgacgcca ccatactcgt cgacgatcaa tccgaaggat agcttgcgct
     2521 ggcggaaatc gtggaacagt tcgaccgccg gttttgattc gggaaagaag ttcggttgtc
     2581 gcagcagctt gcggaaaccc tggggatcac gttccagtct cgcaagatcc accccgagca
     2641 gatcacgcgt gtgcaggatc ccgaggatcg tatcacgatg ccccttgaat accggatagc
     2701 gtgactggcg gctggccttg atctgggcaa tcagtcccgc caccggcaga tccgccgaga
     2761 gaaactgaat gtccgagcgt ggccgcatga tgtcgcgcag cgatttgttg ccgaactcga
     2821 agatcttttc gatgtattgt gcttcctggc tgtcgagcgc gccttcgccg acggcgtcgt
     2881 gaaccagcgt gcgaatcatg tcctcggtca ccaggttacc ctgcgagcgt tgcttgccga
     2941 cgatcagggt aatgaagaaa tccgagatgt accgtatcac ttcgcgtaat ggggtgatca
     3001 ggcgggcgac gagcgcgatc gggcggcact cgacgttggc gaatgccacg ttattgcgaa
     3061 tggccaggac cttcggggtg atttcgccga acagcagcag gatcggcacc atcaccaaca
     3121 ggttgatcat ttcgttttcg gcgccaaaga ggtggatgat cattgccgcc gagattaccg
     3181 atgcggagac attgacgaac tcgttgccga tgaggatggt gacgatcagg cgccgtggct
     3241 cgctgcgcag ccgctcgatc aaatcgatgc gcggattctt ggcggcgcgc atctgttcaa
     3301 gctggaaagt actgagggag aagagcgagg tctcggtact tgaaaagaaa cccgagaacc
     3361 ccagcagaac gataaaaacg acgagctcta tccagagtgc gggatccatg catatctcct
     3421 gagtggcttg cctgtgctca ccagaaaccg gcgcgcgggt gccaaaggcg gttcgatgga
     3481 aggctcaagg ttgccggcga tgcgctcctg ccggctttgt gtttgcattg cgcgaatatc
     3541 tggttaatgg cgacgaagtg ctgttgttcg atccggtggc cctgcttcac agaagcggcg
     3601 agccattttc tcggagtttg atgcctgggt caatggttcg aagtctg
//
LOCUS       DAORHD010000372         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28156_length_3641_cov_3.249861, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000372 DAORHD010000000
VERSION     DAORHD010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28156_length_3641_cov_3.249861"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKO06_08970"
     CDS             complement(<1..315)
                     /locus_tag="PKO06_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69815.1"
                     /translation="MAISSYDSIIASIVAGKSQDRMFSKTLSGNLAVGQALSFWEVGN
                     TPAAGAGGTALTGRNCDPTVTGALTFANPTSPDTQHLICGYAYGTSSAMGTLMVYDRL
                     ADV"
     gene            complement(325..1074)
                     /locus_tag="PKO06_08975"
     CDS             complement(325..1074)
                     /locus_tag="PKO06_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69816.1"
                     /translation="MAVTLKDMKWYKTTGGLGGAITATELLSQTATLSASIAGVTVID
                     AADNALGAGTLLWDASESAFYYTPPSGTIGSAVVVSGTGEIAVRGAGSNAGYIRLSIT
                     FGSLPASDATRTVTVANDAGELFDDVTKAEALVGSTEYRCIYLKNTHGTDAIAGAAVW
                     INADSVGGDSIAIGLDPAGVGATGATIASETTAPAGVVFSVPLLEAAALQIAAMTAGQ
                     SVAIWIRRTVPAVTNTSVADDYSWLRYRILV"
     gene            complement(1075..3102)
                     /locus_tag="PKO06_08980"
     CDS             complement(1075..3102)
                     /locus_tag="PKO06_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69817.1"
                     /translation="MTTAITHITLADSEGDLFIGIAKVRAADLYARLERAKQLIGSHR
                     FEYPHRDKTFYIEIRVQMPHVHIYIDSKTCPTFLSGLVWIKSFFTPTVTVDDVEYLRT
                     MFKKDAPTVEENVSKLAASAGQLKTMRSGQFSGEMRKVVQILQGTGREVPYSPTSGTT
                     HGVFRTTGGPWVIKISSDGVVAYPMKTCRGKNSPTSLGYTPLPTAEPEEPRVLMTAEA
                     IKDAYTGKRAFYGLCGWAFSSDGSKAANCFVGSKDMYSYAWQYQISITADDDGQPISA
                     SMARLTEGYIHGPKTSHMKYPRSDIPTALYSFDPYRGNANYRNDCSAPAFCYYDGGQL
                     QTFFYNYAPGESFSRTDAGDGRSGLCTEFDGVQRFFGSYSENAQPVIRLNSRRGSRSV
                     RDRRSRQTIYTSGPLGMGNELPDFPSSSVVEVWFWNRERETARDNVTGLETSVLIVTG
                     YEREAAYIAEVLASDSGGFSASLDQHAFPKSLYTKEPVEPCYATSDLSHISFTVGAFE
                     GSPYNDDSCQSGKRYGELFGYWVFPGTGVGSLITWREQVEDGWNGCRNLVQQNATFYA
                     ANGSDVATWEYDIPRTRSTDYKLSLYASDAGRVAVNASNAEVDDWIDFIEQGMNDQLA
                     QVVRDAFAPSRMAFSRAPNKARGYELQTSSQFGPYSLDAVTSTTILFVGEP"
     gene            complement(3068..3313)
                     /locus_tag="PKO06_08985"
     CDS             complement(3068..3313)
                     /locus_tag="PKO06_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69818.1"
                     /translation="MSILYQGMQTPTDLRIRKTANEGYVVEYVMYDPKAEQPCHRQGV
                     KGAVYDSLESAMVDVHEYLKKGKGCLCDDCDHAHHAG"
     gene            complement(3316..>3641)
                     /locus_tag="PKO06_08990"
     CDS             complement(3316..>3641)
                     /locus_tag="PKO06_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69819.1"
                     /translation="EGNYTPIAGDTMATFVASSTESTAYAEAARPAFVDSTAAGGATS
                     NSSSKAEFTMNATKTIYGAFLSSVTTKSATSGVLLSAAKFSTAKVVDSGDILRVTVTL
                     TLTSS"
BASE COUNT          732 a         1134 c         1061 g          714 t
ORIGIN      
        1 cacgtcagca aggcggtcgt acaccatcaa ggtccccatc gcagaggatg tgccgtaggc
       61 atagccgcag atcagatgct gcgtgtccgg cgaagttggg ttggcgaaag tcagcgcacc
      121 cgtgaccgtc ggatcgcagt ttcgccccgt gagggccgtc ccgcccgcgc cggctgccgg
      181 cgtattgcca acctcccaga acgacagcgc ctggcccacc gcgaggttcc cggacagtgt
      241 tttagaaaac atcctgtcct gggacttccc agcgacgatc gaagcgatga tggagtcgta
      301 agacgaaata gccatgtcca gtcctcaaac gagaatgcgg tagcgaagcc atgagtagtc
      361 gtccgccacc gaggtattgg taacagcggg caccgtgcgg cggatccaga ttgcgaccga
      421 ctggccagcc gtcatcgcgg cgatctgaag cgcggcggcc tctagtagtg gaactgagaa
      481 cacaacgcct gcaggcgcag tcgtctcgct ggcgatcgtg gcgcccgtgg ccccgacacc
      541 ggcggggtcg agcccgatgg caatcgaatc accaccgact gaatccgcgt tgatccagac
      601 ggccgcgcca gcgatcgcat cagtgccgtg cgtgttcttg agatagatgc agcggtactc
      661 agtggatccc acaagcgctt ccgctttcgt cacatcgtcg aacagctcgc ccgcatcgtt
      721 ggccaccgtg acggtgcgcg tcgcgtccga ggccggcagc gagccgaagg tgatggagag
      781 ccgaatgtag ccggcattgc tacccgcacc gcggacggca atctcccccg tgccagacac
      841 caccactgca gacccaatcg tgccgctggg cggggtgtag taaaacgcgg actcagacgc
      901 atcccagagc agcgtgccgg cgcccagcgc gttgtcggca gcgtcgatca cggtcacgcc
      961 cgcgatgctg gccgagagcg tcgcagtctg cgacagcaac tccgtcgcgg tgatggcgcc
     1021 gcccaggccg cccgtggtct tgtaccactt catgtctttc agcgtgacgg ccatttacgg
     1081 ttccccaacg aagagaatgg tggtgctggt gacagcgtcg agtgaatacg gaccgaattg
     1141 cgaagaggtc tgcaattcat accctctcgc cttattcggc gcgcgcgaaa acgccatgcg
     1201 gctaggggcg aatgcgtcgc gcacaacctg cgctagttgg tcattcatcc cctgctcgat
     1261 gaaatcgatc caatcatcga cttcagcgtt cgaggcattc actgcgacgc gcccggcatc
     1321 cgatgcgtac aacgagagct tgtaatccgt cgatcgcgtg cgaggaatat cgtattccca
     1381 ggtcgcaacg tcggagccat tagccgcgta aaaggttgca ttctgctgca cgaggttgcg
     1441 gcacccgttc cacccgtctt cgacctgctc ccgccacgta atcagtgagc cgacgccggt
     1501 ccccggaaac acccaatagc cgaaaagctc tccgtagcgc ttacctgact gacaagagtc
     1561 gtcgttatac ggcgacccct caaacgcacc gacggtgaaa ctgatgtgag acagatcgct
     1621 ggtcgcgtaa cacggttcga cgggctcttt tgtgtagagg ctcttcggga aggcgtgttg
     1681 gtcaagagaa gccgaaaacc ccccggaatc tgacgcaaga acctcggcta tatacgcagc
     1741 ctcgcgctca tacccagtca cgatcaaaac actcgtttcc aggccggtca cattgtctcg
     1801 cgcggtttcc cgttcgcgat tccaaaacca cacctctaca accgaactag aaggaaaatc
     1861 cggtaactcg ttgcccatgc ccagggggcc ggacgtgtag atcgtctgac gcgatcgccg
     1921 gtcccttacg ctgcgcgagc cgcggcgaga gttcaggcga atcactggct gggcgttctc
     1981 tgagtaagat ccgaaaaatc tttgaacccc gtcaaactcg gtgcacagcc ctgaccggcc
     2041 atcccccgcg tcagttcgcg agaacgactc gcccggcgcg tagttgtaga aaaaggtttg
     2101 aagctgcccg ccgtcgtagt agcagaacgc cggagcagaa caatcgttgc gataattggc
     2161 gttgcctcga tacgggtcga agctgtacag cgccgtcggg atgtcgctac gcggatactt
     2221 catgtggctc gttttcggcc catgaatgta gccctccgtc agccgcgcca tcgaggcaga
     2281 aatgggctga ccgtcgtcgt cggctgtgat cgagatctgg tactgccagg cataggagta
     2341 catatccttc gagccgacaa agcagttcgc cgccttgctg ccgtccgagc tgaacgccca
     2401 accgcacagc ccgtagaacg cgcgcttccc ggtgtacgca tccttgatcg cttcggcagt
     2461 catcaacacg cgcggctctt cgggttctgc tgtcggcaac ggggtgtaac cgagcgaagt
     2521 cggtgaattc ttgccccgac acgtcttcat cgggtatgcc accacaccgt cgctcgaaat
     2581 cttgatgacc cagggccccc cagtcgtacg aaacacgccg tgcgtcgttc cactggtcgg
     2641 cgaatacgga acctcgcggc ccgtcccttg caggatctgc acaaccttgc gcatctcacc
     2701 ggagaactgc ccgctccgca tggttttcaa ctgacctgcg ctcgcagcca gcttcgaaac
     2761 attctcttcg acagtcgggg cgtccttctt gaacatcgtg cggaggtatt caacgtcgtc
     2821 cacggtgact gtcggcgtga agaagctctt gatccacacc aggccgctga gaaacgtcgg
     2881 gcatgtcttg ctgtcgatgt agatatgcac gtgcggcatc tgcacgcgga tctcgatgta
     2941 gaacgtctta tctcggtgtg gatattcaaa gcggtgcgag ccaatcaact gcttggcccg
     3001 ctcaaggcgg gcgtagagat ccgcggcacg cactttggca atgccgatga acaggtcgcc
     3061 ttcggaatca gccagcgtga tgtgcgtgat cgcagtcgtc acagagacag cccttgcctt
     3121 tcttcaggta ctcgtggacg tcgaccattg ccgactcaag actgtcatac acagcccctt
     3181 tgacgccctg tcgatgacac ggctgttctg ccttcgggtc gtacatgacg tactcgacga
     3241 catagccttc gttcgccgtc ttccggatcc gcagatctgt aggcgtctgc atgccttgat
     3301 agagaatgct catgctcagc tgctcgtcag ggtcagggtc acggtaacgc gcaggatgtc
     3361 cccactgtcg acgaccttgg cggtcgagaa cttggcagca gacagcagta cgccgctcgt
     3421 tgcgctcttg gtcgtcacag acgacaggaa tgcgccatag atggtcttgg tcgcattcat
     3481 ggtgaactcg gccttgctcg acgaattcga cgtcgcgccg cccgctgctg tggaatcgac
     3541 gaacgcaggg cgcgcagcct cggcgtacgc cgtcgactca gtcgaagacg cgacgaaggt
     3601 cgccatggtg tcgccggcga tcggcgtgta gttgccttcg a
//
LOCUS       DAORHD010000373         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28203_length_3637_cov_2.777778, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000373 DAORHD010000000
VERSION     DAORHD010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28203_length_3637_cov_2.777778"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PKO06_08995"
     CDS             <1..852
                     /locus_tag="PKO06_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV69820.1"
                     /translation="GKSIIEILVKSGFEVYLIDWGYPTDADKNDGLDVHVNLYIDRMV
                     DKVRELSRQDQVTLLGYCMGGAMSMMYTALHQEKVKNLVTLATPFDFGASENILSKWG
                     RDLPVEDIVNTFGNCPGWFLNMGFDAMKPFDKIDKVINITDGIMDDDFRTLFTAMEKW
                     VSDAVSVPGKAYEEFLRGGFRENRLMKNEFCVGEQMVDLKKITCSYLNLVAEKDTLVL
                     PVSSLKIGDYLGSEDKKLMTSNTGHIGLAVSGKTLKTLWPEAAAWMKERSGGNRKVAK
                     RLVEQTS"
     gene            974..1468
                     /locus_tag="PKO06_09000"
     CDS             974..1468
                     /locus_tag="PKO06_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69821.1"
                     /translation="MFTPHMTYWNDMMDKVREMYNTSLKTNQQVQNEFNNLLKEIIQQ
                     TVKNTVGFQKETENITQKLTETYFDNINKITNYQIENSEKTYQTIMGLMDTYGIHNKE
                     FRAEIEKIWKTNTTTYRGKVDEMNTILRTNQEKYLQTVFETWRNAIKEMEQIHNRVDA
                     ANTK"
     gene            1536..1919
                     /locus_tag="PKO06_09005"
     CDS             1536..1919
                     /locus_tag="PKO06_09005"
                     /inference="COORDINATES: protein motif:HMM:NF021246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(R)-hydroxyalkanoic acid synthase subunit
                     PhaE"
                     /protein_id="HNV69822.1"
                     /translation="MSENTHVIHEMFEAWQKMNANVSRSFFELAAEPYKTESYEQLYR
                     CWANGLTEVIEKAMHMPQCSKTAWENFKPMANMYKAGTQITETMLRNMKMPTHSDIAE
                     LSQRLKDLDDRLERLEKLEKKSAQA"
     gene            2040..3221
                     /locus_tag="PKO06_09010"
     CDS             2040..3221
                     /locus_tag="PKO06_09010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNV69823.1"
                     /translation="MRLPEVYLRTYRSSFHQWIYAKMVRRPDRNLAPGTLVRVLNKDG
                     SFTCIGIYNPLSTIAIRVLSITDAPTGPVFFRERIKAARRLREDILQLTAHSDSYRLI
                     HSESDGLPGLIIDRYGSHFLVKPYSSGYQGQVMDSIVEALCAVFPGAQVHVASDEKAC
                     TRDHVSYVAEERKYPVKGARPLITENDLSFQVDLAGGQKTGYFLDQKLNRKLAADVAR
                     GEDVLDLYCYTGGFGIHCLKSGAKSAIGVDLDPLALEIARQNARRNGVTMEFHCLDVF
                     EHLKETVASGKKYGLVIADPAKLASVKAEVSRAVKTFGDLNELAIRAVKPGGLLFTFS
                     CTGMISGKIFQSVVFTAAQNARANLRVLYNVGPSPDHPYSSAFPEGRYLHGLLMQVDP
                     A"
     gene            3257..>3637
                     /locus_tag="PKO06_09015"
     CDS             3257..>3637
                     /locus_tag="PKO06_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69824.1"
                     /translation="MEQVKESANGGSLPDIRSLVLFVTISYGPAWLGLLVVLWKGLAG
                     GAFGAVFQMLLLYGTTLPIVWLAFIGVRRFGLGHAAFWILFLSWQVYGVLLGLAVASA
                     FADGAVSQTLLLEQKMLFALTLLVP"
BASE COUNT          893 a          929 c         1010 g          805 t
ORIGIN      
        1 ggcaagagca tcatcgagat tctcgtgaag agtggcttcg aagtctatct gatcgactgg
       61 gggtatccga ccgatgccga caagaacgac ggtctcgacg tgcatgtgaa cctgtatatc
      121 gaccggatgg tcgacaaggt gcgcgaactg agccgtcagg atcaggtcac gctgctcggt
      181 tactgcatgg gcggtgccat gtccatgatg tataccgctc tgcatcagga gaaggtgaag
      241 aacctggtga cgctggcgac accgtttgat ttcggcgcct ctgaaaacat tctctccaaa
      301 tggggccggg acctcccggt tgaagacatt gtcaatacct tcggcaactg ccccggctgg
      361 tttctgaaca tgggattcga cgccatgaag ccgttcgaca agatcgacaa ggtgatcaac
      421 atcaccgatg gaatcatgga cgacgatttc cgcaccctgt tcaccgccat ggagaagtgg
      481 gtcagcgatg cggtgtcggt gcctgggaag gcctatgaag aatttctgcg cggcgggttc
      541 cgcgagaatc ggctcatgaa gaatgaattt tgcgttggcg aacagatggt tgatctcaag
      601 aagatcacct gctcgtatct gaacctggtt gccgaaaagg ataccctggt tctgccggta
      661 tccagtctga agatcggtga ttaccttggg agtgaggata agaagctcat gacctcgaat
      721 accggccata tcggtctggc ggtcagtggc aagaccctga aaaccctgtg gcctgaagcg
      781 gcggcctgga tgaaggagcg ttctggcgga aatcggaagg ttgccaagcg gcttgtagag
      841 cagacttcct gagaatttga aaatttcttc gcaagtgcga aataaatgat tgactttggt
      901 cgatagatga gttagtatat aagtgtccga tgaacgaaaa aacaaaatca ataaaaaaat
      961 aggagatagc ccgatgttta cgcctcacat gacctactgg aacgacatga tggacaaggt
     1021 tcgcgaaatg tataacacct ctctcaaaac caatcagcaa gtgcagaatg aattcaacaa
     1081 cctgttgaaa gaaatcattc agcagaccgt gaaaaacacc gttggttttc agaaagaaac
     1141 cgaaaacatc actcagaagt tgaccgaaac ctacttcgac aatatcaaca agatcaccaa
     1201 ttaccagatc gaaaacagcg agaagaccta ccagaccatc atgggtctga tggacaccta
     1261 cgggatccac aacaaggaat tccgcgccga gatcgaaaag atctggaaga ccaacaccac
     1321 cacctatcgc ggcaaggtcg atgagatgaa cactatcctg cgcaccaacc aggaaaagta
     1381 cctgcagacc gtgtttgaaa cctggcgcaa tgcgatcaag gaaatggaac agatccacaa
     1441 ccgcgttgac gcggccaaca ccaagtaacc tctgacctct gacggggccc gttttcgggc
     1501 cccgtttttc gagtcgcgcc ccaaggagat cacccatgtc tgagaacacc catgtcattc
     1561 acgaaatgtt tgaagcctgg cagaagatga acgccaatgt ttcgcgcagc ttttttgaac
     1621 tcgcggcgga accttacaag accgagagct acgaacaact ctatcgctgc tgggccaatg
     1681 gcctgaccga agtgatcgag aaagccatgc atatgccgca gtgcagcaaa acggcgtggg
     1741 aaaatttcaa gcccatggcg aacatgtaca aagcaggcac ccagattacg gaaaccatgc
     1801 tgcggaacat gaagatgccc acccacagcg acattgctga actgtctcag cgtctcaaag
     1861 atctcgatga tcgtctcgag cggctcgaaa agctggaaaa gaagtccgct caagcctgac
     1921 agcccaattc ccttgccgtt cggcaaggat tcttcatcac catacggagc gcgcattggc
     1981 gcgcttcttt tttttcggtg gaaaaaagtg ctaccatgag gctccggagg tcttctctca
     2041 tgcgtctgcc cgaagtctat ctgcgcacct atcgttcctc gttccaccag tggatctacg
     2101 ccaagatggt caggcggcct gaccgcaacc tcgcccctgg cactctcgtg cgggtgctga
     2161 ataaagatgg ttccttcacc tgcatcggta tctataatcc cctcagtacg attgcgattc
     2221 gagtgttgtc gattaccgac gcccccaccg gaccggtgtt tttccgtgaa cgaatcaaag
     2281 cggccagacg cctgcgtgaa gacattctgc agttgacggc gcacagcgac agttatcgat
     2341 taatacacag cgagagcgat gggctgcccg gcctgatcat cgatcggtac gggtcacatt
     2401 tcctggtaaa gccctacagt tccgggtatc aggggcaggt catggattcg attgtggaag
     2461 cactctgcgc ggtatttccc ggggcgcagg tgcatgttgc ctctgacgag aaagcctgta
     2521 cgcgtgatca cgtcagctat gtggccgagg agagaaagta tccggtgaaa ggtgcgagac
     2581 ccctgatcac cgagaacgat ctgagttttc aggtcgatct ggcgggtggg cagaaaacgg
     2641 ggtattttct cgatcagaag ttgaatcgca agctggccgc cgatgtggcg agaggcgaag
     2701 acgttctgga cttgtactgt tataccggtg gctttggcat tcattgcctg aagagcgggg
     2761 caaaatcggc gatcggtgtt gatcttgacc cgctggcact cgagattgcc aggcagaatg
     2821 cccgacgcaa cggggtgacg atggaatttc actgtctcga cgtgtttgaa catctgaaag
     2881 agaccgtcgc gtcgggaaag aaatacgggc tggtgatcgc cgacccagcc aagctcgctt
     2941 cggtcaaggc cgaagtcagc cgggcagtga aaaccttcgg agatttgaat gagcttgcca
     3001 ttcgtgccgt gaaaccagga ggactgttgt tcacgttctc gtgtaccggc atgatcagcg
     3061 gcaagatctt tcagtcagtg gtattcaccg cggcacagaa tgcgcgtgcc aatctgcggg
     3121 tgctgtataa cgtcggaccg tcacccgatc atccgtattc ttctgcgttt ccagagggtc
     3181 gatatcttca tggcctgctg atgcaagtcg atccggccta ggacacaagt tactgaggct
     3241 gatgagagga ggagtcgtgg aacaggtgaa agaatcagca aacggcggat ctctcccgga
     3301 tatccgctcc ttggtcctgt ttgtcaccat ctcctacggg cctgcctggt tggggttgct
     3361 ggtggtgctc tggaaagggc ttgccggcgg ggctttcggg gcggtctttc aaatgcttct
     3421 cttgtatggg acaacacttc ccatcgtctg gctggcgttc atcggtgtcc gacgtttcgg
     3481 gctcgggcac gcggcattct ggattctctt tctctcgtgg caagtctatg gagttcttct
     3541 cgggctcgca gtagcctcag cgtttgcgga tggggcggtg tcacagacgc tgcttctgga
     3601 acagaaaatg ttgtttgccc tgacactgct ggtaccg
//
LOCUS       DAORHD010000374         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28241_length_3634_cov_3.235261, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000374 DAORHD010000000
VERSION     DAORHD010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28241_length_3634_cov_3.235261"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="PKO06_09020"
     CDS             complement(<1..987)
                     /locus_tag="PKO06_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69825.1"
                     /translation="MGSTDIQAILPRLKPLLAILISNFRKGDYTQRRESAIALSKFKG
                     EKATDVLLQTYESDNIQDFMALALGNVENKRAVQLLINALNDPNSEVRFNAARALGMM
                     NNDEAFNVLVESLNAYADMISAGGGPKQQNQVFFEEEAIISAISALGKLRNTLATPLL
                     KRLLTLEKTGRIRATIIIALGQMQSDRLLPIFQGALKDEDDRVRANSIEAIEAIKSSA
                     IVGILQPYLEDPSNRVRANVAKAIWKYGDYDVTETLVRMLGEKDKWQRASAAFALGEI
                     KAAKFIGKLALALKDEDADVRRNAVNALKKIESASALPHLEAIIDDPSPDVRV"
     gene            1075..2148
                     /locus_tag="PKO06_09025"
     CDS             1075..2148
                     /locus_tag="PKO06_09025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV69826.1"
                     /translation="MAHENSLYDLLKLTVERKASDLHLKIGTPPAIRIDGTVTPVEEF
                     RFSRRDFDRLLETLLSADQRAQFQQSMELDFAYALEGIARFRVNLYQDVYGVGAVFRV
                     IPRDVLNFEDVGLPPAAQKLIEQPNGLILVTGPTGAGKTTTLASYIQYVNQNARRHIV
                     TIEDPIEYQFEEELSIVTQRQVGIDCLGFYEGLLTVLRQDPDIVVVGEMRDPKTIALT
                     LNAAETGKLVLATLHTTSAVQTAERIVNVFPENQQRQVLHQLSMVLRGVVSQTLLPRI
                     GGGRVAAFEVLINTSAVRAIIGDGKIHLLPSFLETGTEIGMTTLEMSMFQLVKRGLVR
                     QEEAMSASPNPAAFKKLLQGARL"
     gene            2145..3212
                     /locus_tag="PKO06_09030"
     CDS             2145..3212
                     /locus_tag="PKO06_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV69827.1"
                     /translation="MTQPRLKSSGTLRIHRFFDLAIQRGASDLHLCAGSPPTLRIGGR
                     LVALPEEPLAGQEMPNLFLPLLDVRMQECLQKNLEVNCMSAYNNSWRVRICVYRQKGC
                     LSGAFRFIPYKIPTVDNLNLPSKLKEIALLPRGLFLVTGPANSGKSHTLAAIVNEINK
                     STARHVITIEDPIEFVHRPRRSIFTQREISTMTHDYQAALIHALRQDPDVILVGEMRD
                     LQTIEMVLTAAETGHFVLSTLHTVGAVETVDRIINVFPGHRHEEIRAQLSLSLIGLIS
                     QILLPGRNRTDRVMAYELLIMNPAMRNMVRERKTNQLKSALMLARKEGCVTLKDSLAE
                     LIRDERIEAKLVSNLIKEIVE"
     gene            3209..>3634
                     /locus_tag="PKO06_09035"
     CDS             3209..>3634
                     /locus_tag="PKO06_09035"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69828.1"
                     /translation="MTFGTELESFRFTKASRRLLRPVTERLAAMIDEDNEAEVKFQCL
                     MALGHIGDPVSLPTMQKALDLYPDNPEPIVALGHYRNSSPVPKLLKLLENPDFEFKEE
                     VVRTLGEIADPMSGKALLQLLQDPERMVRYYAAWALFRLG"
BASE COUNT          750 a         1086 c         1016 g          782 t
ORIGIN      
        1 aacccgtacg tccgggctgg ggtcgtcgat aatagcctcg agatgaggta aagcgctggc
       61 tgattcgatt ttcttcagcg cattgacggc attgcgacgc acgtcggcgt cttcgtcttt
      121 cagcgcgaga gccagtttgc caatgaactt ggccgctttg atctcgccca gagcgaaggc
      181 agccgaggct cgctgccatt tgtccttttc gcccagcatg cgcacgagcg tctcggtcac
      241 gtcgtagtcg ccgtatttcc agatcgcttt ggcgacgttg gcacgcacgc gattactcgg
      301 atcttcgaga tacggctgca gaatgcccac tatagcgctc gacttgatcg cctcaatggc
      361 ttcaatggag ttcgcccgca cccggtcgtc ctcgtctttc aacgcgccct ggaaaatggg
      421 gagcagacga tcggactgca tttgtccgag tgcgatgatg atggttgcgc gaatacgacc
      481 ggttttctca agggtcaaca atcgtttcag caaaggcgtg gccagcgtgt tgcgcagttt
      541 gcccagcgcg gaaatcgccg agatgatggc ttcttcctcg aagaagacct gattctgctg
      601 cttcggcccg cctccggccg agatcatgtc ggcatacgcg ttcagagatt cgacgagcac
      661 gttgaacgcc tcgtcgttgt tcatcattcc cagcgcacgc gcggcattga agcggacttc
      721 ggagttaggg tcattcaagg cattgatcag cagttgcacc gcacgtttgt tttcgacgtt
      781 ccccagagca agcgccatga aatcctgaat gttgtcgctc tcgtaggtct gcagcaacac
      841 gtcggtggct ttttcgccct tgaacttgga aagcgcgatt gccgactcgc gacgctgggt
      901 gtagtccccc ttgcgaaagt tcgagatcag aatagcgaga agcggcttca gtcgcggcag
      961 aattgcttga atgtcggttg atcccatgcc tgtttcctgt tgattttggc acaaagtgta
     1021 tcacacacgt gaaggtgtgg aaagtcattt cgggagtgat atactgtctt catcatggca
     1081 cacgaaaaca gcctgtatga tctgctcaag ttgaccgtcg agaggaaggc gtcggatctt
     1141 cacctgaaaa tcgggactcc gccggcgatt cgcatcgatg gaaccgtgac ccccgtcgaa
     1201 gagttccgct tctcgcggcg cgacttcgac cgtctgctcg aaactctgtt gtccgctgat
     1261 cagcgcgcac agtttcagca gagcatggag ctcgactttg cctacgctct cgagggaatt
     1321 gcgcgtttcc gcgtgaacct gtatcaggat gtctacggcg tcggcgccgt ctttcgtgtg
     1381 attccgcgcg atgtgctcaa ttttgaagat gtcggtctgc cacctgcagc gcagaaactc
     1441 atcgagcaac ccaacggcct gattctggtc accgggccga ccggagccgg aaaaaccacc
     1501 acccttgcga gctacattca gtatgtgaac cagaatgcgc gacggcacat cgtcaccatc
     1561 gaggacccga tcgagtatca gttcgaagag gaactgtcga tcgtgacgca gcgtcaggtc
     1621 ggcatcgatt gtctggggtt ctacgagggg ctgctgaccg ttctgcgaca agacccggat
     1681 atcgtcgtcg tcggcgaaat gcgcgacccg aaaaccattg cgttaacgtt gaatgcggct
     1741 gaaaccggaa aactcgtgct cgcgaccctg catacgacat cagcggtaca gactgctgaa
     1801 cgtatcgtca acgtctttcc cgaaaaccag cagaggcagg ttctgcacca gctctcgatg
     1861 gtcttgcgcg gggtcgtgtc gcagacgctg ctgccgcgca tcggcggcgg gcgggtggcg
     1921 gctttcgaag tgctcatcaa cacctcggcg gtgcgcgcca tcatcggtga cggaaaaatt
     1981 cacctgctcc cgtccttcct cgagaccggc accgagatcg gtatgaccac cttggaaatg
     2041 tccatgttcc agctggtgaa acgcggcctg gtacgccagg aagaagccat gtccgcttca
     2101 ccgaatccgg ccgctttcaa gaaactgctt cagggagcac gcctgtgacg cagccacgtc
     2161 tcaagtcttc cggcacgctt cgtattcacc gctttttcga tctcgccatt cagcgcgggg
     2221 cttccgatct gcacctgtgc gcaggcagcc cgccgacgtt gcgcatcggc ggtcgtctgg
     2281 tggcacttcc cgaagagcct ctggcaggcc aggagatgcc caacctcttt ctgccactgc
     2341 tcgacgtgcg catgcaggaa tgtctgcaga agaatctcga agtcaactgc atgtccgcct
     2401 acaacaacag ctggcgcgtg cgcatctgtg tctatcgtca gaaggggtgc ctgtcaggag
     2461 cattccgctt cattccgtac aagattccga ccgtcgacaa tctgaatctg ccctcgaaac
     2521 tgaaagagat cgccctcctg ccgcgcggtc tgtttctcgt caccggcccg gcaaacagcg
     2581 ggaaatcgca tacgctcgcg gccatcgtca acgagatcaa caagtcgacg gcccggcacg
     2641 tgattaccat cgaagacccg atcgagttcg tgcatcgccc ccgccgctcg attttcaccc
     2701 agcgcgagat ctcgaccatg acccacgatt atcaggccgc cctgattcac gccctgcgac
     2761 aggatcccga cgtcattctg gtcggcgaaa tgcgagatct gcagacgatc gagatggtgc
     2821 tgaccgcggc cgaaaccggt cacttcgtgc tttcgacact gcacaccgtc ggcgcggtcg
     2881 agacggtcga ccgcatcatc aacgtctttc ccggccatcg ccacgaagag attcgcgcgc
     2941 aactgtcgct ttcgttgatc gggttgatct cgcagattct gcttccgggt cgcaatcgta
     3001 ccgatcgcgt catggcgtat gagctgctca tcatgaatcc ggccatgcgc aacatggtac
     3061 gtgagcgcaa gaccaatcag ctgaaatccg cgctgatgct ggcgcgaaaa gagggctgcg
     3121 tgacgttgaa agacagcctg gccgagctga tccgcgatga acgcatcgaa gcgaagctgg
     3181 tcagcaacct gatcaaggag atcgtcgaat gaccttcggg acggaactcg aatccttccg
     3241 ctttaccaaa gcctcgcgaa gattgctgcg cccggtcacc gaacgactcg cggccatgat
     3301 cgacgaagac aacgaagccg aggtcaagtt tcagtgcctc atggcgcttg ggcatatcgg
     3361 cgatcccgtg tctctgccta ccatgcagaa agcactcgat ctctacccgg acaatccaga
     3421 acccatcgtc gctctcggtc actatcgcaa cagttctccg gtgccgaaac tgctgaaact
     3481 gctcgagaac cccgacttcg agttcaagga agaggtcgtg cgcacgctcg gcgagatcgc
     3541 cgatccgatg tccgggaaag ctctgctgca actgcttcag gacccggaac gcatggtacg
     3601 ctattacgcg gcctgggcgc tgttcagact cggc
//
LOCUS       DAORHD010000375         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28338_length_3626_cov_3.258471, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000375 DAORHD010000000
VERSION     DAORHD010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28338_length_3626_cov_3.258471"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="tilS"
                     /locus_tag="PKO06_09040"
     CDS             complement(<1..1095)
                     /gene="tilS"
                     /locus_tag="PKO06_09040"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV69829.1"
                     /translation="MTRPEGFSPAIREWLTSLAHNVPPSLRRKTLGVAVSGGADSVAL
                     FLTLLQLRGRAEFSLCVLHVDHNLRPDSADDAAWVESLAKTHDVPCFRTRLASPDPLS
                     LRKEGLESWARRERLKWLTQQVHTRNIDTVVFGHHRQDQAETVLWRLLRGCGTAGLGG
                     LRPFRRLVTESGTINIWRPFLNTSPETLRRFLAEAGQDWREDPTNAATTFLRNRIRHQ
                     ILPYLSEFQPQIVRHLSALGNTMTEVHRALAARAHRYLSSSSPRTLFWKGSPTGPVLR
                     ELLRQWWIRELPEQAARFNLSTANRLEDLLVQNRSGRKLDLGFVQVARVPSGLEIIDS
                     TIHSPKPRTALLFDSPVECHGQRITLHTSAA"
     gene            1199..2032
                     /locus_tag="PKO06_09045"
     CDS             1199..2032
                     /locus_tag="PKO06_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69830.1"
                     /translation="MDQRNNQETPADISESSPPPLFELRAPTEARAHAQKMATRVNLG
                     LIALMILCALLFGGGDLQTTLRYLSEYSTNLMLGAVFVGVLQLFSRRFSSSSQAWYSC
                     HEDRLVFHWPPRREDILFEEIRELRHLPVPAVAWIDVIDAFCLYLRLKNGREIRLLGL
                     TGLRRVWDYLSEKFPEKVVEQSRASDQVSADILPLRTLVWWFFLGGLWSLTCFLLLSE
                     NLAVAIMLLGLAMAPTEYLIRYYRNGRPASEDPQAFACLSFLIACFCGAVGLIALMLL
                     D"
     gene            complement(2392..>3626)
                     /locus_tag="PKO06_09050"
     CDS             complement(2392..>3626)
                     /locus_tag="PKO06_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69831.1"
                     /translation="KIRTTLQSLLGTGSFTGTHIFTGHADVPDDGGLRLVVLSPVDSF
                     SKRGPSPAEEVCAQILKNRGQQPRQRQNRLIFLVAEQENTPRLWDQVKTLLAWKSITD
                     DCKEGRLNLDQLQAKQADKNLKEAEDVFQSVVRETWRWIMAPFQEAQVKGGISDLRWE
                     HFQLNTSAPKMMSEIERQLKDNELVINEWAPIHLAGLLKKWFWRDDTHEIGALDVWQK
                     MGNYLYLPRLRDESVYRRTLEAGVPSRDFFGIAYGKTDQKYEGFSFGKPTSLAIDGAL
                     LLIEPSAAAAFEAAQKPPEVVKPDPVPPDGKPVPPTAPGGGKGKPEPTPPGPKKGPAM
                     KRFFGSVEVDPVKAKIELAKIVDEVLVHLASKHGVNVTVTLEINAEAPQGFDEATQRT
                     AKENGRLLNFKTLSFEEE"
BASE COUNT          678 a         1004 c         1111 g          833 t
ORIGIN      
        1 tgccgcggac gtatgcaggg tgatacgctg accatgacac tcgacggggg aatcaaagag
       61 cagggcggtt ctcggtttcg gtgaatggat tgttgaatcg atgatttcga gcccggaagg
      121 aaccctggct acttgtacaa atcccagatc cagttttcgc cccgaccggt tctgcacaag
      181 caggtcttcc agccgattcg ccgtcgaaag gttgaagcgg gcggcctgtt ccggtaactc
      241 gcgaatccac cattgccgca ggagttcgcg cagaaccggg ccggtcggcg accctttcca
      301 gaacagggtg cgtggggagg aggagctcag atatcgatgt gcccgcgccg ccagtgcgcg
      361 atgcacttcg gtcatggtat ttccaagtgc gctcagatgg cgaacaatct gcggctgaaa
      421 ctcggacaaa tacgggagaa tctggtggcg aatccggttg cgaagaaacg tcgttgcggc
      481 attggtgggg tcttcacgcc agtcctggcc agcttcagca agaaaacgcc gcagagtttc
      541 gggtgaggta ttgagaaacg ggcgccagat gttgatggtg cctgattcgg tgaccagacg
      601 tcgaaatgga cgcaatccgc caagcccggc cgtcccgcag ccgcggagca ggcgccagag
      661 aaccgtttca gcctggtcct gccggtgatg accgaaaacg accgtgtcga tgttgcgtgt
      721 gtgaacctgc tgagtcagcc acttcagacg ctcacgacgg gcccagctct ccagaccctc
      781 tttgcggaga ctcagcggat caggtgaagc aagtcgggtt cgaaagcacg gcacgtcatg
      841 agtcttcgcc agagactcga cccaggcggc gtcgtcggca ctgtcggggc gcaggttgtg
      901 atcgacgtgc agaacgcaga gactgaactc cgcccttccc cgcaactgaa gcagagtgag
      961 aaatagagcg actgaatccg ctcctccgga aaccgcgact cccagcgtct tgcgccgcag
     1021 agaaggtggg acattgtggg cgagagacgt cagccactcg cgaatcgccg gtgagaagcc
     1081 ttcgggccgg gtcatgtgtc cgtctccgtg ctggtttgct tgcagggcgc gaacgagcag
     1141 gtgacagtat accattcacc ggtgactggt ttcggtttat actcagaaga tcatgcaggt
     1201 ggatcagagg aacaatcagg aaacgccggc cgacatttcg gagtcatccc cgccgccgct
     1261 gttcgaactg cgggcgccga ctgaagcgag agcgcatgcc cagaaaatgg cgacgcgggt
     1321 aaatctcggg ctgatcgcgt tgatgattct gtgcgctttg ctatttggcg gcggcgatct
     1381 gcagacgacc ctgcggtatc tctcggaata ctcgacgaat ctgatgctgg gtgccgtctt
     1441 tgttggggtg ctccagctct tctccaggag attttccagc tcgtctcaag cctggtacag
     1501 ttgccacgaa gaccgattgg tctttcattg gccgccccgt cgagaagata ttctctttga
     1561 ggaaatacgt gagcttcggc atcttcctgt gccggccgtc gcctggattg acgtgatcga
     1621 tgcgttctgc ctgtatctgc gcctgaagaa tggccgggaa attcgactgc ttggcctcac
     1681 cggtttgcgt cgggtgtggg actatctgtc ggagaagttc ccggagaagg tcgtcgaaca
     1741 gagtcgtgca tctgatcagg tttcggcaga cattctgccg ctgagaaccc tcgtttggtg
     1801 gttctttctg ggcggcctgt ggagtctgac gtgttttctt ctgttatcag agaatctggc
     1861 cgtggcgatc atgcttctgg ggctggcgat ggcgccgacg gaatatctga tccgatatta
     1921 ccggaatggc cgaccggcat ctgaagaccc tcaggctttt gcctgcctga gttttcttat
     1981 cgcctgtttc tgcggagcgg ttggcctgat tgccctcatg cttctcgatt gaccttcgaa
     2041 tggcccggcc agctccggtt gcttgagtgc attactctat cgccggtttt tataccttct
     2101 tttataccaa acgcgaccct cagagcaggc gaacgtgtct gattctcgaa tgggaaaaag
     2161 caaaaaaccc gctcaatgag cgggttttgc aatgtttcga ggtcttgtga ttcgacctcg
     2221 caatagcaag ggagggagtc gaacgtcatc tgaaatgcct ttgtgggggc tggtgccgtg
     2281 gttcttgtgt agcctgatat acccatcggt atacccatga gggtccggat gccctccgga
     2341 aattcctcgc ccgacattcg ctctgagacc agtataacac atgctttcgg gtcactcctc
     2401 ctcgaaactc agtgttttga agttcaggag gcggccattt tccttggcgg ttcgctgggt
     2461 ggcttcatcg aagccttgcg gggcttcggc gttgatttcc agggtgacgg tgacgttcac
     2521 gccgtgtttc gaggcgaggt gaaccaggac ttcgtcgacg atcttggcca gctcgatctt
     2581 ggccttgacg gggtcaacct cgacactgcc gaagaagcgt ttcatcgccg gccctttctt
     2641 cgggcctggc ggtgtgggct cgggcttgcc tttgccgcca cctggtgctg tcggcggaac
     2701 aggctttcca tctggcggaa ccgggtcggg tttgacaacc tccggtggct tctgcgccgc
     2761 ttcgaatgcc gccgccgcgc tcggttcgat caggagcaga gcaccgtcga tggcgagaga
     2821 ggtcggcttg ccgaagctga acccttcgta tttctggtcg gttttgccgt acgcgatccc
     2881 gaagaagtcg cgactgggaa ccccggcttc gagggtgcgc cggtagaccg attcgtcacg
     2941 caggcggggc agatacaggt agttacccat cttctgccag acgtcgagag cgccgatctc
     3001 gtgagtgtcg tctcgccaga accatttctt caacagcccg gccagatgaa tcggtgccca
     3061 ctcgttgatg accagctcgt tatccttgag ctggcgttcg atctcgctca tcatcttcgg
     3121 agcgctggtg ttgagttgaa aatgttccca gcgcagatca ctgattccgc ctttgacctg
     3181 agcctcctgg aagggggcca tgatccagcg ccaggtctcg cgaaccacgc tctggaaaac
     3241 atcttcggct tccttcaggt tcttgtcggc ctgttttgcc tgcagttggt cgaggttcag
     3301 acgtccttcc ttgcagtcgt cggtgatcga cttccaggcc agcagggtct tgacctggtc
     3361 ccacaggcgc ggggtgtttt cctgctcggc gaccagaaag atcagacgat tctggcgctg
     3421 acgaggttgt tggccacggt tcttgagaat ctgggcacag acctcttccg ccgggcttgg
     3481 cccgcgcttc gagaacgagt caaccgggga cagcacgacg agtcgcaggc cgccatcgtc
     3541 gggcacatcg gcgtgaccgg tgaagatatg cgtgccggta aacgagccgg ttccgagcag
     3601 gctctgcaac gtcgttcgaa tcttcg
//
LOCUS       DAORHD010000376         3625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28350_length_3625_cov_3.935294, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000376 DAORHD010000000
VERSION     DAORHD010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28350_length_3625_cov_3.935294"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKO06_09055"
     CDS             <1..834
                     /locus_tag="PKO06_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV69832.1"
                     /translation="HLGDQGVFCQLQGNLFFGTTDQLYSQLEPDLRTRRYLLLDMRHV
                     LSLDYTAAHLLQMMEARLKERDGRLLFSGMPSGLHDRRNFEEYLSQTGVLQSSSGMMI
                     FETKNDALVWIESRLLEAAGVCPEADEAPLALHEFDLFREFDAEALRALESCAQPVSY
                     PAGSCIFRKDDAGDELYLVRRGLISVLLPLESGKRHHLADIGRGSYVGELSFLDRRTR
                     SAEAVARRDTDLLVLSRTRFDAVVHSSALVGVKVFARLALVIAERLRQADVELESLED
                     R"
     gene            complement(863..1450)
                     /gene="rdgB"
                     /locus_tag="PKO06_09060"
     CDS             complement(863..1450)
                     /gene="rdgB"
                     /locus_tag="PKO06_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNV69833.1"
                     /translation="MKTLWVASRNRHKLQEIAAILGPNVTLKSLLDRPDIPDVDENAS
                     TYLENARLKAQAIWAAVREPVVADDSGLEVDALGGRPGVHSARYSAPNPSAAKNITKL
                     LGELGDLPAEKRSARFRCTVVYFDESGREHTFAGTLEGRIGTAPCGAGGFGFDPVFWL
                     DDRGCMVAELSDDEKNRISHRGRAFAQLKTFLQVP"
     gene            complement(1447..2178)
                     /gene="rph"
                     /locus_tag="PKO06_09065"
     CDS             complement(1447..2178)
                     /gene="rph"
                     /locus_tag="PKO06_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018046444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNV69834.1"
                     /translation="MPRHDGRTFNQLRPVTIEPGYVKYPHGSAQISFGDTRVLCTVMV
                     DEKVPGFLESRSEPQGWLTCEYALLPASGLTRNSRAGYGSTTVKGRVHEIQRLIGRAL
                     RAAIDLTKIGKRTLMIDCDVIQADGGTRTASVTGAMVALELAVQRLMKEGKLTESPIV
                     AQVAAVSVGLLDGEPVLDLDYKEDSAAETDLNLVMTRAGGYIEVQGTAEDGTYSRKQL
                     DQMLNLGEEGIRQLFALQDKALGRK"
     gene            complement(2198..2419)
                     /locus_tag="PKO06_09070"
     CDS             complement(2198..2419)
                     /locus_tag="PKO06_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69835.1"
                     /translation="MDEDVNVTPPDLKEKPSKRDRHAKREFEAPKPKFDMEAEHRKFL
                     MIMFGLFVIVLVVMLVMNQHVDFAMDGGM"
     gene            2595..3056
                     /locus_tag="PKO06_09075"
     CDS             2595..3056
                     /locus_tag="PKO06_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69836.1"
                     /translation="MISIKGKVEKGKGESRNTIREQMPFFKDCFPEVADCQHGTLNIR
                     LEKPLIILHPDFTTEPVPWHPALRISKKGEVFQFVRARLAIDGQEPTPVWIYRAQFSP
                     YRDDPYVAEVLAPPLKFSGHPSCRLELATNAFEGIVIKGENRQQKGRKAPA"
     gene            3053..>3625
                     /locus_tag="PKO06_09080"
     CDS             3053..>3625
                     /locus_tag="PKO06_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV69837.1"
                     /translation="MNVLRLQRLTDRVQIIPGPTIIGVILLSDDRVAIIDSGLDRRAG
                     RRLYELLRDYHYKLDVILNTHAHADHIGGNSFLHRTTQCRILAGPLEAPVIRHPLIQA
                     VTLFGGAPPKELQNRMLTGDASPAEDIRGDSFELDGLKIDVIPLPGHSIGQVGFVVDG
                     VAFLADALFAPSIMEKHKLVYMYDPGQQRQT"
BASE COUNT          701 a         1149 c         1054 g          721 t
ORIGIN      
        1 cacctgggcg accagggggt gttctgtcag cttcagggca acctgttctt cggcacaacc
       61 gatcagctct attcccaact ggaacccgat ctgcgcacgc gacgctacct gctgctggat
      121 atgcggcacg tcctgtcgct cgactacacc gccgctcatc tgctgcagat gatggaagcc
      181 cgtctgaaag agcgcgacgg acggctgttg ttcagcggca tgccctcggg cctgcacgac
      241 cgacgcaact tcgaagagta cctgtcgcag acgggcgtat tgcaatcctc aagcggaatg
      301 atgatctttg aaaccaagaa cgacgctctg gtctggatcg aatcacgcct gctcgaagcc
      361 gcgggagtct gcccggaagc cgacgaagcg ccgctggccc tgcacgagtt cgatctcttt
      421 cgcgagttcg atgccgaagc gctgcgcgcc ctggaatcct gcgcgcagcc cgtctcgtat
      481 cctgccgggt cctgcatctt caggaaggac gacgcgggtg atgaactcta tctggtgcgg
      541 cgtgggctga tcagcgtgct tctgcctctc gagagcggca aacgccacca cctcgccgac
      601 atcggtcggg gcagttacgt tggcgaactg tcgtttctcg atcgacgaac ccgttccgcg
      661 gaagcggtcg cccgccgcga taccgatctg ctggtactct cgcgaacgcg cttcgatgcc
      721 gtggtccatt cctcggctct ggtcggggtc aaggtctttg cgcgcctggc gctggtgatt
      781 gccgaacgcc tgcgacaggc tgacgtagag cttgaaagcc tcgaagatcg ctaggacatt
      841 tcaccgggat cggtgacgcc tgtcagggaa cctgcaaaaa cgttttcaac tgcgcaaacg
      901 cccggccacg atggctgatg cgattctttt cgtcgtctga cagttcggcc accatacagc
      961 cgcgatcatc gagccagaag acggggtcga acccgaatcc gccggcaccg cacggcgctg
     1021 tgccgatgcg cccctcgagc gtgccagcga acgtgtgctc gcgacccgat tcatcgaagt
     1081 agacgaccgt acagcgaaac cgcgccgagc gtttctcggc aggcagatca cccagttcac
     1141 ccaggagctt ggtgatgttc ttcgcggcac tggggttcgg agccgagtag cgggcggaat
     1201 ggacgccggg gcgccctccg agggcatcga cctcgaggcc agaatcatcg gcgaccaccg
     1261 gctcgcgcac ggcggcccag atggcctgcg ccttcaggcg ggcattctcg agatacgtcg
     1321 acgcgttctc gtcgacatcg gggatgtcag gacgatcgag caggctcttc agcgtgacat
     1381 tcgggcccag aatcgcggcg atctcctgca gtttgtggcg attgcgcgag gccacccaga
     1441 gcgtcttcat ttgcgtccca gggctttgtc ctgcaaggca aacaactggc gaatgccttc
     1501 ctcgccgaga ttcagcatct gatcgagctg ctttcggcta taggtgccgt cttctgccgt
     1561 cccttgcact tcgatgtacc cgccggcacg agtcatgacc aggttcagat cagtctctgc
     1621 ggccgagtct tctttataat cgagatcgag caccggctcg ccgtcgagca gtccgacgct
     1681 caccgcggcc acctgagcca cgatcggact ttccgtgagc ttgccttctt tcatcagacg
     1741 ctgaaccgcg agttcgagtg cgaccatggc tccggtcacc gaagcggtgc gggtgccgcc
     1801 atccgcctga atgacgtcgc agtcgatcat cagcgtgcgt ttaccgattt tggtcagatc
     1861 gatcgcggct cgcaacgcgc gcccgatcag gcgctgaatt tcatgcaccc ggcccttgac
     1921 ggtcgtcgac ccatacccgg cgcgcgagtt gcgggtcaac cccgaggccg gcaggagcgc
     1981 atactcgcag gtcagccagc cttgcggctc tgaccgtgat tcgagaaacc cgggcacctt
     2041 ctcatcgacc atcaccgtgc agagcacgcg tgtgtcgccg aagctgatct gggccgaccc
     2101 gtgcggatac ttcacgtatc cgggttcgat ggtgaccggc cgcaactggt tgaacgttct
     2161 tccgtcatga cgtggcatgg aactttcctt tcgggggtta cattccgccg tccatggcga
     2221 aatcgacgtg ctgattcatg accagcatga ccaccaacac aatgacaaac agaccgaaca
     2281 tgatcatcag gaacttacgg tgttccgctt ccatgtcaaa cttcggcttg ggtgcctcaa
     2341 actcccgctt ggcgtgacga tcacgcttgc tgggtttttc cttgaggtcg gggggggtga
     2401 cattgacgtc ttcgtccacg gcggcactcc tgaaaggggt tcgatgagat cgctccggaa
     2461 tccgagtcga gaaccagaac ggcgcaacga attcggcccg gggcttccag agcataccat
     2521 cggccggggg gggttgccaa gagccacggt tttgactatg ctgaagcgac ccgacgtctc
     2581 atcggagagg atacatgatc agcatcaaag gcaaagtcga gaagggaaaa ggggaatcac
     2641 gaaacacgat tcgtgaacag atgccctttt tcaaagactg ttttcccgag gtcgcggact
     2701 gccagcatgg caccctcaac atcagactgg aaaaaccgct catcatactg cacccggact
     2761 tcaccaccga accggttccg tggcacccgg ccctgcgcat cagcaagaag ggcgaagtgt
     2821 ttcaattcgt gcgcgcccga ctggcgatcg acggccagga acccaccccc gtctggatct
     2881 accgcgccca gttttccccc taccgtgacg acccgtatgt cgccgaggtc ctggccccgc
     2941 ccctgaagtt ttctggccac cccagctgcc gtctcgaact ggcgaccaac gccttcgaag
     3001 gcatcgtcat caagggcgag aaccgccagc agaaaggtcg gaaagcgccg gcgtgaacgt
     3061 cctgcggttg cagcggctca ctgaccgggt tcagatcatt cccggaccaa caatcatcgg
     3121 ggtgattctg ttgtctgacg atcgagttgc catcattgat tccggtcttg accggcgcgc
     3181 gggacgacga ctctacgaac tgctgcggga ttatcactac aagctcgacg tcattctcaa
     3241 tacccatgca cacgccgacc acattggcgg caactcgttt ctgcaccgca ccacgcagtg
     3301 ccgcattctc gccgggccgc tcgaagcgcc ggtcatccgg cacccgctga ttcaggccgt
     3361 caccctgttc ggcggcgcgc cgccgaaaga gctgcagaac cgaatgctga caggtgatgc
     3421 cagccccgcg gaagatattc gcggggattc ttttgaactc gacggtctca agatcgacgt
     3481 catccccttg cctggtcact ccatcggtca ggtcgggttt gtggttgacg gggtcgcctt
     3541 tctggccgat gcgctctttg ccccgtcgat catggaaaag cacaaactgg tctatatgta
     3601 tgaccccggc cagcaacggc aaacc
//
LOCUS       DAORHD010000377         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28436_length_3619_cov_3.950898, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000377 DAORHD010000000
VERSION     DAORHD010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28436_length_3619_cov_3.950898"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="PKO06_09085"
     CDS             <1..488
                     /locus_tag="PKO06_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69838.1"
                     /translation="LLGVIDSARNRSFVFSQTGQLLSELSGPTEACFYQNELVQLQIS
                     GNRVHARVIKRGGSTDSLFTAETTPGNVWLDAQAIGAIGSTLVVIGRQGQGDEDHPVW
                     SRVLVYRQGTVRSCSVIPHLGLHAGSPKSYTLLTVDGAQSLIDLDQHPEGIAVRAFRL
                     P"
     gene            570..2015
                     /locus_tag="PKO06_09090"
     CDS             570..2015
                     /locus_tag="PKO06_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV69839.1"
                     /translation="MASSLSRSLDQVRVAVQSSVPLSWTLDIPSEEHLHALSQNLAQI
                     LDELQKPELKHPIVFCFHELFTNAHKALAKRVFFRRRELSLENQTQYREGMTLFRQDW
                     MQNIDYWNHEVEREGLRIQVVFQWSNGVLMVVVANSTSVLPWELERIQAQKTGAMNLG
                     ESEVMENLDQTEGGGYGIRLIYSILHKLGCPVSCFSLISQTDRTIALFKVPLASLAVH
                     RTPVSSRLAEEVKQLPAFPETILILQQKLLDPKVEMSSLVKIIEQDPNLVAALLKTAN
                     SAAYRRLQPISGIKEAVTLIGLKGLSGLLYAFGSEVALKGRHQPLLTEHAQATATFAH
                     QIAKNYAPKEADTVFAGGLLHDLGKIIISTYQTRFQEICKEMVREQRLPEWFVENLSF
                     GLDHAELGGLVAQHWNFPAGLVEMMRYHHNPSQAREARRATAIVSLANLIPRIADGSY
                     PLYLLDPAIFEELPGLSEEHVLKLAQTLTKH"
     gene            complement(2038..3348)
                     /locus_tag="PKO06_09095"
     CDS             complement(2038..3348)
                     /locus_tag="PKO06_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HNV69840.1"
                     /translation="METSRLAIRVRRWWRSLLLLLALVGPGLITATADNDAGGIATYS
                     LAGALFGYDLLWTMIPITILLILTMEMSARLGAVTGQGLADLLRERFGVKVTFWVLFM
                     VLIANFGTVISEFSGIAASIEIFSTGFGYGQEFIYLKYFLIPFIAWVLYKIITEYDYK
                     KIEKVFLLSIFFYLAYPISAWKANPNWGEAIKALFVPVWRFDAPYLALMIGSIGTTIT
                     PWMQFYLQSTLIEKGVTVKNYREAKYDVILGSLTTVFVAFCIVVACAVNICFDPRHGP
                     INSIEQVGAALIPLAGPNAGFLFAFGLFNASIFGAAILPLSTAFAVCEGMGWERGMNK
                     TYEEAPFFYWIFRFLLFSGALIVLVPGMSFVYLGIMRVSQVAQGLILPPFLYYLVRLG
                     DDKNLLGEHVNGPWVSRISWICVYVLTVIDLLMLASMAKEVIIG"
     gene            complement(3358..>3619)
                     /locus_tag="PKO06_09100"
     CDS             complement(3358..>3619)
                     /locus_tag="PKO06_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV69841.1"
                     /translation="GVVSIRDLLVSGPATQIKAIMADKLIFVQSDTPKEEVASLISKY
                     DLLAMPVLDEDGTIMGVVTVDDVIELLLDHMPRVWKRRALSS"
BASE COUNT          878 a         1084 c          936 g          721 t
ORIGIN      
        1 cacttctagg agtcatcgac tcggcccgca atcgaagctt tgtcttctca cagaccggac
       61 aattgctctc cgaactttca gggcccactg aagcctgttt ttatcagaac gaactcgtac
      121 aacttcagat ttccggcaac cgcgttcacg cccgagtgat aaagcgaggg ggaagcacgg
      181 actcactgtt caccgccgag accacccccg ggaacgtctg gctcgatgcc caggccatcg
      241 gagctatcgg ctcaacgctc gtggtcatcg gccggcaagg acaaggagac gaagaccacc
      301 cggtgtggtc gagagtgctc gtctatcgcc agggaaccgt gcgcagctgt tccgtcattc
      361 cccatcttgg cttgcatgcc ggatcaccca aatcctacac acttctcact gtcgatggcg
      421 ctcagtcgtt gatcgatctt gatcaacatc cggaagggat cgccgttcgt gcgttccgtc
      481 tgccctaaat tgacattcgc tgaacacccg cttaccatgc ggatagaggc acgtgcaaat
      541 tcatttgtcg tctgtgtgag gtcgagagta tggcatcgtc tctgagccga agtctggatc
      601 aggttcgtgt cgctgttcag agcagcgtgc ccctcagttg gactctcgac atcccatctg
      661 aagaacatct gcacgccctc tcgcaaaatc tggctcagat tcttgacgag ctgcagaagc
      721 cggaactgaa acacccgatc gtcttctgct ttcacgagtt gttcaccaac gcccacaaag
      781 ctctcgccaa gcgggtcttc ttccgacggc gcgaactgtc gctcgaaaac cagacccagt
      841 atcgagaagg catgaccctg ttccgtcagg actggatgca gaacattgac tactggaatc
      901 acgaagtcga gcgcgaaggg ttgcgcattc aggtggtttt tcagtggagc aacggcgttc
      961 tgatggtcgt ggtcgcgaat agtaccagcg tgctcccctg ggaactcgaa cgtattcagg
     1021 ctcagaagac tggtgccatg aatctcggcg aaagcgaggt catggaaaac ctggatcaaa
     1081 cggagggtgg gggatacggc attcgtttga tctactcgat tctgcacaag ctcggatgcc
     1141 cggtaagttg cttttcgctg atctcgcaga cagatcgaac tatcgcgctg ttcaaggttc
     1201 cgctggcttc tctggccgtt catcgtacgc cggtcagctc acgactggcg gaagaagtca
     1261 aacaactgcc ggccttcccc gagacgattc tgattctgca gcagaaactg ctcgacccaa
     1321 aagtcgaaat gtcctcgctc gtgaagatca tcgaacagga cccgaatctg gtggcggcgt
     1381 tgctcaagac ggccaactcc gcggcctatc gccgattgca gccaatatcc ggcatcaagg
     1441 aagcggtcac gctgatcggt ctcaaggggc tctccggcct gctctacgcc ttcggttcag
     1501 aggtcgcact gaagggacgg catcagccgc tgctcaccga acacgcacag gccaccgcca
     1561 ccttcgctca tcagatcgcc aagaactatg cgccaaaaga ggctgacacg gttttcgcgg
     1621 gcggtctgct gcacgacctt ggcaagatca tcatctcgac ctaccagaca cgctttcagg
     1681 aaatctgtaa agagatggtc cgcgaacaac gactgccgga atggtttgtc gaaaatctct
     1741 cgtttggtct tgatcacgcc gagcttggcg gcctggtcgc ccagcactgg aactttcccg
     1801 ccggtctggt cgagatgatg cgatatcatc acaacccgtc gcaggcacga gaagcgaggc
     1861 gggcaacggc catcgtctct ctcgccaatc tgattccgcg catcgccgac ggttcatatc
     1921 cgctctacct gctcgacccg gcaatcttcg aagaactgcc cggtctgtca gaagaacacg
     1981 tgctgaaact cgctcagact ctgaccaaac actgaacggt caatccatgt ttccgtccta
     2041 gccgataatg acctctttag ccatcgaggc cagcatgagc aggtcgataa cggtcaacac
     2101 atagacacag atccaggaga ttcgactgac ccaggggccg ttcacgtgct caccaagcag
     2161 attcttgtca tcgcccaatc gtacaaggta atagagaaac ggcgggagaa tcaacccctg
     2221 cgccacctgc gacacacgca tgatgccgag atacacgaag ctcattcccg gcaccagaac
     2281 aatcagcgcg ccagagaata gcagaaagcg gaagatccag tagaaaaagg gagcttcctc
     2341 atacgtcttg ttcattccgc gctcccagcc catcccttcg cagacggcaa aggcggtgga
     2401 cagcggcaga atggcggcac cgaagatgga ggcgttgaac agtccgaacg cgaacagaaa
     2461 acccgcattc ggccccgcca acggaatcaa agccgcgccg acctgctcaa tcgaattgat
     2521 cggcccgtgg cggggatcga aacagatgtt cacggcacac gccacgacga tacaaaaagc
     2581 gacaaacacc gtggtcagcg aaccaaggat aacatcgtac ttcgcctcgc ggtaattctt
     2641 gaccgtcacc cccttctcga tcagcgttga ctgcagatag aactgcatcc agggcgtgat
     2701 ggtcgtgcca atcgaaccga tcatgagcgc cagatacgga gcatcgaacc gccacacggg
     2761 gacgaacaac gctttgatcg cctcacccca gttcgggttc gccttccagg cagagatcgg
     2821 atatgccaga tagaaaaaga tcgacaggag aaaaaccttt tcgatcttct tgtaatcata
     2881 ctcggtgatg attttgtaga gaacccaggc gataaacgga atgagaaaat acttcaggta
     2941 gatgaattcc tgaccatagc cgaacccggt gctgaaaatc tctatcgatg cggctatacc
     3001 cgagaactcg ctgatgaccg tgccgaagtt cgcgatcaac accatgaaca gcacccagaa
     3061 cgtcaccttc acgccaaaac gttcacgcag caggtcagca agaccctggc ccgttaccgc
     3121 acccaggcgt gcgctcattt ccatggtgag aatgagtaga atcgtgatcg gaatcatcgt
     3181 ccagagcagg tcgtaaccga aaagcgcccc ggccagcgaa taggttgcga ttccccccgc
     3241 gtcgttatcc gccgtcgccg tgatcaatcc ggggccgacc agagccagca acagcagcag
     3301 actcctccac cagcgccgca cccgaatcgc gagacgagag gtttccatgg gctgtgctca
     3361 actcgacaac gcgcgccgct tccacacacg cggcatatga tcgagcaata actcgatcac
     3421 gtcatcgacg gtcacgactc ccatgatggt tccgtcttcg tcgagcacgg gcatcgccag
     3481 aagatcatac ttcgaaatca gcgatgccac ttcttctttc ggcgtatcag attgtacaaa
     3541 tatcagtttg tccgccatga tggctttgat ctgagttgcc ggaccggaga ccagcagatc
     3601 gcgaatcgag accacgccc
//
LOCUS       DAORHD010000378         3618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28449_length_3618_cov_4.315464, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000378 DAORHD010000000
VERSION     DAORHD010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28449_length_3618_cov_4.315464"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PKO06_09105"
     CDS             complement(<1..262)
                     /locus_tag="PKO06_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNV69842.1"
                     /translation="MGVATFLSRILGLVREQAFAFWFGAGIWMDAFNVAYRIPNLLRD
                     LFAEGALSSAFVPTFNGVLQKGGKEGAFRLARLVLSTLLVVVG"
     gene            462..1964
                     /locus_tag="PKO06_09110"
     CDS             462..1964
                     /locus_tag="PKO06_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69843.1"
                     /translation="MSRLLIWSVLFWCLTGFGLTNPAEAGWFKLTETKPTGQTTASPA
                     QQVGQTKTPVRRGWFKSYHAAPEPASETTSDEGTETEPAQPADEEPADEPGDETTEAA
                     SSDEETGDETTDTDSEPIIPVASLGAWLPVVEPGGEIFPSLHLVLSLITMSRDDIELQ
                     TEDQQTSDNSPEVLGDQDGMIGIVVRSPADQYRVKVTVACPTLMSPGTVELVLPQKGL
                     EYIVFPRIVWDFDKLLACRQTVPANLTFTVTGDGRKPEERVETVRVRTINDCPYFYID
                     GETSLDLNWMFTAYVNEDHPFIDRVLQEALQSGVVKRFNGYQDGDSKEVVRQVFALWH
                     TLRAQGIKYSSITRTPSQSDSVLSQHVRFVDQAIENTQANCVDGSILFASLLSKIDLD
                     PFLVVLPDHAFIGFHLNPDHTEQAFLETTMLSEVSSETADLFPELDEELTAKWRNSSS
                     WREFRRAAADGAEQFEEQKTKLEGSDPMYQVIDLEEARRRGIMPIAYRKP"
     gene            2051..>3618
                     /gene="glgB"
                     /locus_tag="PKO06_09115"
     CDS             2051..>3618
                     /gene="glgB"
                     /locus_tag="PKO06_09115"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HNV69844.1"
                     /translation="MKPTVSAEAIQAIINTDYHDPFQVLGAHPIQVGGKPAVAVRAFL
                     PDAIAAQVVDAQGSSRYTMQKLHDAGFFEAVIEERGEVFPYRLGAEYATGATAEFYDS
                     YAFTPTLGDMDLYLFGEGNHHKIYEKLGAHLRVMKYFDHSVGGIGFAVWAPNAKRVSV
                     IGDFNMWDGRRHVMRSLGSSGVWEIFVPGLQMGQNYKFEIKWKNGNLVEKIDPFGFYF
                     EVRPKTAAKIYDINNYQWNDQGWINDRPRRNWHREAVSIYEVHLGSWMRVTEEGNRFL
                     TYRELASRLVEYVKKMGYTHIELMPVSEHPLDISWGYQVTGYYAPTSRFGNPKDFMYF
                     VDTMHQNGIGVILDWVPAHFPKDWHALAQFDGSALFEHLDPRLGEHKDWSTLIFNFGR
                     NEVKNFLIANALFWIDKYHLDGLRVDAVASMLYLDYSRKHGEWIPNKYGGRENLEAIE
                     FLKYLNSICYQKFPGVMMVAEESTAYPGVSKPCDQGGLGFGFKWNMGWMHDTLSYFQK
                     EPIHRKFHHSNLTFP"
BASE COUNT          798 a         1110 c         1003 g          707 t
ORIGIN      
        1 aaccgacaac caccaagagc gttgacagta caagtcgtgc aaggcgaaaa gcgccttctt
       61 ttcccccttt ctgcagaacg ccgttgaacg tcggtacgaa ggcggaactc agagcccctt
      121 cggcgaacag atcacgcagc aggttgggaa tgcggtacgc gacattgaac gcgtccatcc
      181 agatgccggc gccgaaccag aaggcgaaag cctgctcgcg caccaacccg agaatgcggc
      241 tgagaaacgt cgcgactccc atcgcccccg ccgagcgagc gatggatgaa gatgcagagg
      301 ttgggttctc agaggatggg ttcgcggatg cctcggtcat agatgaggcc ttattgtgtc
      361 acagatcttc cccggacgga agagggataa ccaatttcct tgccgaaacc aggggatcgc
      421 cgctactatg actcggcaaa tcaagtcttt caggagctca catgtctcgc ctgctcatct
      481 ggtccgtgtt gttctggtgt ctcaccggtt tcgggctcac gaatcctgcc gaggccggtt
      541 ggttcaaact gaccgagacg aagccaacgg ggcagacgac cgcgtcaccg gctcaacagg
      601 tcggccaaac caagactccg gttcgtcgcg gatggttcaa gtcttatcat gccgcgccgg
      661 aacccgcttc agaaaccaca tcggacgaag gcacggagac ggaaccggcc cagcctgccg
      721 atgaggaacc cgcggatgag ccaggcgatg agacgacaga agccgcttcg tcagatgaag
      781 agacgggcga cgaaaccacc gacacggact ctgagccgat cattccggtc gcttcgctcg
      841 gggcctggct tcccgtcgtc gaaccgggtg gagagatttt tccctcactg catctggttc
      901 tctcgctcat caccatgtcg cgcgacgata tcgagctgca aaccgaagat cagcagacct
      961 ccgacaactc tcccgaagtg ctcggcgatc aggacgggat gatcggtatc gttgttcgct
     1021 ctccggccga tcagtatcgg gtgaaggtga cggttgcctg cccaactctc atgtcccccg
     1081 gcaccgtcga acttgtgttg cctcagaaag gccttgaata catcgttttt ccgcgtatcg
     1141 tctgggactt tgacaagttg ctggcctgcc gccagaccgt tcccgccaac ctgaccttta
     1201 cggtgacagg agacgggcgc aagccggaag aacgcgtcga aaccgtgcgc gtacgcacca
     1261 tcaatgactg cccctacttc tacatcgacg gcgaaacctc gctcgatctg aactggatgt
     1321 tcaccgccta tgtcaacgaa gaccacccgt tcatcgaccg ggtcttgcag gaagctctgc
     1381 aatcgggagt cgtcaagcgt ttcaacgggt atcaggacgg cgacagcaaa gaggtggtgc
     1441 gtcaggtctt cgctctctgg cacacgctgc gcgcccaggg aatcaaatac agttccatca
     1501 cccgcacccc gtcgcagagt gacagcgtgc tcagtcagca cgtgcgcttc gtcgatcagg
     1561 ccatcgaaaa cacgcaggcc aactgcgtcg acggcagcat tctgttcgcc tcactgctca
     1621 gcaagatcga tctcgatccg tttctggtgg tcctgccgga tcatgcgttc atcggctttc
     1681 atctgaaccc cgaccacacg gaacaggcat ttctcgaaac gaccatgctc agcgaagtct
     1741 cttcagaaac cgccgacctg tttccggaac tggacgagga actgaccgcc aagtggcgca
     1801 actcctcttc ctggcgcgag tttcgtcggg cagcggccga cggcgccgaa cagttcgagg
     1861 agcagaaaac caaactggaa ggcagtgacc ccatgtatca ggtcatcgac ctcgaggaag
     1921 cccgacggcg ggggatcatg cccattgcct accgcaagcc ctgaaccgtt caggatcggt
     1981 tcgcgcttcg aagtgaattc catcgcgttt tctggtatat tcgcgcttaa ctcttttgtg
     2041 aggtgggaac atgaaaccga ccgtctccgc agaagccatt caggccatca tcaatactga
     2101 ttatcacgac cccttccagg ttcttggggc gcatcccatc caggtaggcg gcaaaccagc
     2161 cgtcgcggtc agggcgtttc ttcccgatgc catcgccgca caggtggtcg atgctcaggg
     2221 gagcagtcgg tatacgatgc agaagctgca tgacgcgggt ttcttcgagg ccgtcatcga
     2281 agaacgtgga gaggtctttc cctatcgact cggagccgaa tacgccaccg gggcaaccgc
     2341 cgagttctac gattcgtatg ccttcacgcc caccctcggc gacatggatc tctacctctt
     2401 cggcgaaggc aaccatcaca agatctacga aaaactcggc gcgcatctgc gcgtcatgaa
     2461 gtatttcgat cactcggtcg gcggcatcgg gttcgccgtc tgggccccga acgccaagcg
     2521 cgtcagcgtc atcggcgact tcaacatgtg ggacggccgg cgccacgtca tgcgctcgct
     2581 gggttcttcc ggcgtctggg aaatcttcgt tcccggcctg cagatgggtc agaactacaa
     2641 gttcgagatc aagtggaaga atggcaatct ggtcgaaaag atcgacccgt tcggtttcta
     2701 cttcgaagtg cgccccaaga ccgcggcgaa gatctacgac atcaacaact accagtggaa
     2761 cgaccagggc tggatcaacg accgaccgcg tcggaactgg caccgcgaag ccgtttcgat
     2821 ctacgaagtg catctcggct cgtggatgcg cgtgaccgaa gaaggcaacc gctttctgac
     2881 ctaccgtgaa ctggccagcc gcctcgtcga gtacgtcaag aagatgggct acacgcacat
     2941 cgaactgatg ccggtctcgg aacacccgct cgacatctcg tggggctatc aggtcaccgg
     3001 gtactacgcg ccgaccagcc gcttcggcaa cccgaaagac ttcatgtatt tcgtcgatac
     3061 catgcaccag aacggtatcg gcgtcattct cgactgggta cccgcgcact ttccgaaaga
     3121 ctggcacgcg ctggctcagt tcgacggctc ggcgctgttc gagcatctcg acccgcgcct
     3181 gggcgagcac aaagactggt cgaccctgat cttcaacttc ggccgcaacg aagtgaagaa
     3241 cttcctcatc gcgaacgccc tcttctggat cgataaatac cacctcgacg gcctgcgtgt
     3301 agacgctgtc gcctcgatgt tgtacctgga ctattcacgc aagcacggcg aatggattcc
     3361 gaacaagtac ggcggccgcg agaacctcga agccatcgag tttttgaagt atctgaacag
     3421 catctgctat cagaagttcc cgggcgtcat gatggtcgcc gaagaatcaa cggcttaccc
     3481 cggcgtcagc aagccctgcg accagggcgg tctgggcttc gggttcaagt ggaacatggg
     3541 ctggatgcac gacacgctca gctactttca gaaagagccc attcaccgca agtttcatca
     3601 cagcaacctg acgttccc
//
LOCUS       DAORHD010000379         3617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28462_length_3617_cov_3.402302, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000379 DAORHD010000000
VERSION     DAORHD010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28462_length_3617_cov_3.402302"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..400
                     /locus_tag="PKO06_09120"
     CDS             233..400
                     /locus_tag="PKO06_09120"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNV69845.1"
                     /translation="MKKTVHKTRLIDGGFAEEVFSDMLDQERSKGIAIGLGDMLYQQL
                     SRAITQPARKR"
     gene            471..1757
                     /gene="rho"
                     /locus_tag="PKO06_09125"
     CDS             471..1757
                     /gene="rho"
                     /locus_tag="PKO06_09125"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNV69846.1"
                     /translation="MDESVLTYLGLCNEPIKNLKLIAKEFGVTGLIKKDQMIRAILKI
                     HLEKQGLALSEGVLEILPDGYAFIRTRSYVQNDDDVYVSPSQIKRFGLMTGDSLVAQV
                     RLPKDDESYYSLFKIESVNGCSPEEARTKRRNFEDGIPVFPTDRFRLETSDNDICVRM
                     FDLICPIGKGQRGLIVAPPKAGKTTLLKNLANAIIANHPEVLMLILLIDERPEEVTDM
                     RRSVDAEVVASTFDEHISHHIKVSELLLEKAKRYVEMGRDVVILLDSITRLARAYNIS
                     VPSAGQTLTGGVNPFALHKPKRFLGAARKIEGGGSLTVIATTLVDTGSKMDDVIFEEF
                     KGTGNMELHLDRKLFNKRIFPSIDVKMSGTRKEELLMSNEDLVKVIALRRAIDDKSPQ
                     ETVEMLIKSLSATKTNAEFLAMINPNYKPSAKTGNS"
     gene            1898..2470
                     /locus_tag="PKO06_09130"
     CDS             1898..2470
                     /locus_tag="PKO06_09130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV69847.1"
                     /translation="MHPTTIDSAENCWNAVDTCVTESSRSVHRWYTDLAAATPSYRIV
                     SVKQTLAPDVLSWPVYGSLSSGFGSRRHPVTRRHHFHAAIDIRARVGTPVCAPSDGRI
                     VAVHRAGAMGRMVKMVTSTGMVLAFGHLSGYRCHPGQSVRRGQILGLVGASGRATGPH
                     LHFAVQRDGRYVNPLTFLASRTDNQSRHLP"
     gene            2600..>3617
                     /locus_tag="PKO06_09135"
     CDS             2600..>3617
                     /locus_tag="PKO06_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69848.1"
                     /translation="MRNLGLAVVLLLITGSVSTALELHPDVYLEAGSDAALRKYEEGY
                     RSYHEGDYGKAEQKLHESLVLEPNLIKAHYWLGKLYREMGRLKESTFHHEEVLRLKKL
                     IATRREALKFANNEYPSQGQILITREREKRAREAFDRCRRFLDEGQWDAALSEAKKAV
                     DEFPGKLEYLLMLARIHWDRGEKKNSAALYVQLCENGKVDKPIFDEALERLLIEGETL
                     AVRRMLRLWVRRFPTDDEARKTLEQIEMQLQGEPDAEVPAVGRVVRRMDRQVILDFGL
                     DRRLRLADEYALSLRAFQPGETIRDTKGNRFLGREPDRVSGKLLVTRVLARSCWALIQ
                     QEYGA"
BASE COUNT          810 a          954 c         1028 g          825 t
ORIGIN      
        1 agctcgagcc gcagaacgcc aagtttctcg cgtttcagaa acaagcgttc cgcgaaggat
       61 aaccatggat tttctccctc tgcactcagc atcgctggct ccgctcacgg caagaaggtc
      121 gttgcgtggc gcagagtctg agccgacgga agaaaagaaa aaagagctcg aaaaactcaa
      181 gtcagcctgt aaagattttg agtccatttt catgcttcag atgatgaaag aaatgaagaa
      241 gactgttcac aaaactcgcc tgattgacgg agggtttgct gaagaggtct tctctgacat
      301 gctcgatcag gagcgctcga aaggcattgc catcggtctc ggcgacatgc tctatcagca
      361 gctctctcgg gcaatcacgc aacctgcgcg taaaaggtag ttccgtcgac tttttcccgt
      421 cttcttctca gttaaccccg taatataacg ccccccgtgg aggagtgtct gtggacgaat
      481 ccgttttgac gtatttgggt ctgtgtaacg aaccgatcaa gaacctcaag ctcattgcca
      541 aagagtttgg cgtgaccggg ctgatcaaga aagatcagat gatccgggcg attctcaaga
      601 ttcacctcga gaaacagggc cttgcccttt ctgaaggcgt gctcgagatt cttccggacg
      661 gatacgcgtt tatccgcacg cgcagctacg ttcagaacga cgacgatgtg tatgtttcgc
      721 cctcacagat caagcgcttt gggctgatga cgggcgattc tctggtggcc caggttcgtt
      781 tgcccaaaga cgatgaaagc tactattcgc tcttcaaaat tgagagcgtg aacggttgtt
      841 ctccggaaga agctcgtacc aagcgtcgga acttcgaaga tggaattcct gtcttcccga
      901 ccgaccgttt ccggctcgaa accagcgata atgatatctg tgtgcgcatg ttcgacctga
      961 tctgcccgat cggcaagggc cagcgcggtt tgattgtcgc tcctccgaag gctggtaaga
     1021 cgaccttgct gaagaatctg gcgaatgcca tcattgccaa tcacccggaa gttttgatgt
     1081 tgatcctgct gatcgacgag cgcccggaag aagtcaccga catgcgccgt tctgttgacg
     1141 ccgaagttgt tgcttcgaca ttcgatgagc acatctccca tcacatcaag gtttccgagt
     1201 tactgttgga aaaagcgaaa cggtatgttg aaatgggtcg tgatgttgtg atattacttg
     1261 attcgattac gcgacttgcc cgggcctaca acatttcggt gccctctgcg gggcagacgc
     1321 tgacaggtgg tgtaaacccc ttcgcgttgc acaaaccgaa gcgtttctta ggcgcagcgc
     1381 gtaaaatcga agggggcgga agtttgactg ttatcgcaac cacgctcgta gacactggtt
     1441 ccaaaatgga cgatgtgatt ttcgaagagt tcaagggcac gggaaacatg gaactgcatc
     1501 tcgaccgcaa actcttcaac aagcgcatct tccccagcat cgatgtcaag atgtccggaa
     1561 cccgtaaaga agaactgctg atgtcgaacg aggacctggt caaagtgatt gccctgcggc
     1621 gtgccatcga cgacaagagc ccgcaggaaa cggtcgaaat gctcatcaag agtctctcgg
     1681 cgaccaagac gaatgccgaa ttccttgcga tgatcaaccc caactacaag ccttccgcca
     1741 aaaccggcaa ttcgtgaggc gtctcgaaac gcgtccagat cgttcgattc cgtttttctg
     1801 gcgtttcgcg cttctgctct gcgggtggtt gatctttcag atgtgctcgg ttcccgctgt
     1861 ttactgcagt cttgacttca cgagagagca atggtgtgtg cacccaacca ccattgactc
     1921 ggctgaaaac tgctggaacg cggtcgacac ctgcgtcacg gagtcgtccc gatcggtgca
     1981 tcgctggtat accgatctcg ccgcggcaac ccccagttac cgcatcgttt ccgtcaaaca
     2041 gactctcgct cccgatgtgc tttcctggcc tgtgtatggc tcactcagct cgggtttcgg
     2101 ttctcgtcgt cacccggtga cgcgccggca tcactttcac gccgcgatcg atattcgtgc
     2161 ccgcgtggga accccggttt gcgctcccag cgacggacga atcgttgcgg tgcatcgcgc
     2221 gggagccatg ggacgaatgg tgaaaatggt cacctcgacc ggtatggttc tggctttcgg
     2281 tcatctctca gggtatcgct gtcatcccgg ccagtcggtg aggcgtggac agattctcgg
     2341 tctggtcggt gcgtcaggcc gtgccactgg cccccacctg cactttgccg tgcaacgcga
     2401 tggaagatac gtgaatccgc taaccttttt agcgtcgaga accgacaacc agagtagaca
     2461 ccttccataa aaagagtgtc gaattttact ttcctcaggt gtctcggtgg ttgcacaaag
     2521 gttgccagtt tgcaccagta ggtcgatcgc tccaggcgtg gagttcgatt ccccgcgccg
     2581 gatccgtggc tttctgtggg tgaggaacct gggactggcg gtcgtgcttc tgttgattac
     2641 cggttcggta tccaccgcac tcgaactgca cccggatgtc tacctggaag ccggatctga
     2701 tgctgcactg cgaaaatacg aagaaggata tcgcagctat cacgagggtg attatggcaa
     2761 agccgagcag aagctgcatg agtctctcgt gctcgagccg aacctgatca aagctcatta
     2821 ctggctggga aaactgtatc gtgagatggg gcgcctgaaa gaatcgacgt tccaccacga
     2881 agaggttctc cgcctgaaga agctgattgc gacgcgtcgc gaggcattga agtttgccaa
     2941 caacgaatac ccgtcacagg ggcagattct catcacgcgt gagagagaaa agcgggctcg
     3001 tgaagcattc gatcgttgtc gccggtttct tgacgagggg cagtgggatg ccgccttgtc
     3061 tgaggccaag aaggcggttg acgagtttcc gggaaaactc gagtatctcc tgatgctcgc
     3121 tcgcatccac tgggaccgcg gcgaaaagaa gaacagcgcg gcactgtatg tgcagctgtg
     3181 cgaaaacgga aaagttgaca agccgatctt tgacgaagcg ctcgagcgac tcctgatcga
     3241 aggagaaacg ctggccgtgc gtcggatgtt gagactttgg gtgcgacgct ttcccacaga
     3301 cgatgaggcg cgcaagactc tcgagcagat cgagatgcag ttacagggag agcccgacgc
     3361 ggaagtcccc gcagtcggca gagtcgtgcg tcgcatggat cgccaggtca ttctcgattt
     3421 cggccttgat cggcggttga ggctggctga cgaatatgcg ttgtctctgc gggcttttca
     3481 gcctggagaa acgattcgtg acacgaaggg aaaccggttt ctggggcgtg agcctgatcg
     3541 cgtgagcggg aaactgctgg tgaccagagt gctggcgaga tcctgctggg ctttgattca
     3601 gcaggagtat ggagctg
//
LOCUS       DAORHD010000380         3612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28533_length_3612_cov_3.418892, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000380 DAORHD010000000
VERSION     DAORHD010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28533_length_3612_cov_3.418892"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="PKO06_09140"
     CDS             <1..935
                     /locus_tag="PKO06_09140"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69849.1"
                     /translation="SFYLLFTALLGVTAWALGSMLARRFLTPIRELSVAVEAIERREF
                     QHRLPLFTPDELGRLALTFNRVMEGLSDLEVARIVQENLFPKDELRIGEYCLFGRSFP
                     ATELGGDYLDLRLLPEDRVLIVIGDVSGHGVPAALVMALAKGAADVQCEKNAAPNEIL
                     GAMNRALVGSVRRKRFMTCFLAVLDTRRHELCFSNAGHCDPILIGVNGTVEHIVNRNH
                     VLGIKSQTVFSTSTLEVNPGDRIVLYTDGLVETRASTGQIGFSAAQDVLPKLFDDDLP
                     TSCRRMYQWAADQNIAPCQEDDITIVLFARVLPA"
     gene            1158..1832
                     /locus_tag="PKO06_09145"
     CDS             1158..1832
                     /locus_tag="PKO06_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV69850.1"
                     /translation="MKRKSSASKTLDSSKKLYTYADYCTWPDDERWELMDGEAVAMTP
                     GPGPDHQRLSRKFCTQIDTLLEGQTCEMFNAPIDVLLPDADEPDEQVKTVLQPDVLVV
                     CDPSKITRRGIRGGPDFVIEVLSPSSASRDQIRKRRLYERFGVKEYWVVDPEQQLVWV
                     YRRGVKAGKNGVETLTSDFQVVQAFDVDDKAIEIVALPGLTIDLTRIFPPRPKVVRER
                     PRYYSV"
     gene            2021..3343
                     /locus_tag="PKO06_09150"
     CDS             2021..3343
                     /locus_tag="PKO06_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNV69851.1"
                     /translation="MKNLLQELKSKKTALCVVGQGYVGLPLAHAFAKKFRVIGFDVNK
                     EKIALLKKGIDPTGEVPEGGLKEVKIEFTDDPSRIAEAKFIVVAVPTPVDKNKRPDLY
                     PLVASSTTVGKHLQKGSVVVYESTVYPGVTEDDCLPLLEKHSGLKYGRDFSIGYSPER
                     INPGDKEHRLETITKVVSGSDDETLELVAQVYETIIKAGVHRASSIKVAEAAKVIENT
                     QRDLNIALMNELSIIFHKMGIDTREVLAAAGTKWNFLKFFPGLVGGHCIGVDPYYLTF
                     KAEELGYHPEVILAGRRINDGMGKYVAENTVKEMIRVGKHVRGARVLILGLTFKENVP
                     DLRNTRIVDIIAELKEYGVHSYVHDPLADPHEAEEEYSLHLYTDLKKIPPVDVIILGV
                     AHDEYRDLSLKKLVAKCSRGEKAPVLIDVRGLWTQDLARKAGFHYWRL"
     gene            3362..3553
                     /locus_tag="PKO06_09155"
     CDS             3362..3553
                     /locus_tag="PKO06_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNV69852.1"
                     /translation="MTTKMTMIYWKSKKFWLGKILEHPEIMSQGESIEELEGNLKEAF
                     RLMAFDDVPETFQVKDIAV"
BASE COUNT          787 a          991 c         1051 g          783 t
ORIGIN      
        1 ggagtttcta cctactcttt actgcgctgc tgggggtgac agcgtgggcg ctcggcagta
       61 tgctggccag gcgctttctt acacctattc gtgagctttc tgtcgccgtc gaagcaatcg
      121 aacggcgaga gtttcagcat cgattgccgt tattcacgcc tgacgaactc ggacgtttgg
      181 ctttgacctt caatcgcgtg atggaggggc tgtccgatct cgaggtcgcc cgcattgtgc
      241 aagagaatct ttttccgaaa gacgagttac ggatcggcga gtattgcctg tttggtcgat
      301 cgtttccggc aaccgagctg ggcggagact atcttgatct gcgactgctg cccgaagatc
      361 gtgttctgat cgtcattggc gatgtgagtg gtcacggggt tccggcggct ctggtcatgg
      421 ctctggcgaa aggcgcggca gatgttcaat gcgaaaagaa cgccgcaccg aacgagattc
      481 tcggcgcgat gaatcgggcc cttgtcggct cggtccgccg caaacgcttc atgacctgct
      541 tccttgccgt gctggatact cgtcgccatg aactctgctt cagcaatgcc ggacactgcg
      601 acccgatcct gattggggtc aacggaacgg tcgagcatat cgtgaaccgt aatcacgtgc
      661 tcgggatcaa atcacagacg gtcttctcga cctcgacgct ggaagtgaac cccggtgatc
      721 gcatcgtttt atacaccgac ggattggttg aaacgcgcgc gagcacagga cagatcggct
      781 tttctgccgc gcaggatgtg ctgccgaagc tgttcgatga tgatctgccg acatcgtgtc
      841 gacgaatgta ccagtgggcg gcagatcaga acatcgctcc atgtcaggaa gatgacatca
      901 cgattgtctt gttcgctcgc gtcttgcccg cgtgaccgaa acctgtgagc cgaccggcaa
      961 gaggtggact gagtccgaaa tcaggcttga ggatcgcatg aggcaaatcg gtgatatccc
     1021 tcgaagcgat tggttggcaa tcagagcatg tggagcggtc tgacgaacga gagctcaaag
     1081 gcccgtcgcg cgatgaacaa gttcccgttt gcccagggga ttttgacgct ccagcctctc
     1141 tgtggcatac ttccaccatg aaacgaaaat cctcggcatc caaaactctg gattcctcaa
     1201 agaaactcta cacctacgct gattactgca cgtggcctga cgatgagcgg tgggagctca
     1261 tggacggcga ggctgtcgct atgactccgg ggccgggccc tgaccatcag cggctgtcgc
     1321 ggaagttttg cacgcagatt gacaccctcc tcgaggggca aacctgcgaa atgttcaacg
     1381 cgccgatcga cgtgctgctt cctgacgctg acgagcccga cgagcaggtc aagacggtcc
     1441 tgcagcccga tgtgctggtg gtgtgcgatc cgagcaagat tacacgccgt ggcattcgtg
     1501 gcggacccga ctttgtcatt gaagtcttgt cgccctcgtc cgcttcacgc gaccagattc
     1561 gcaaacgtcg tctgtatgag cgttttggag tgaaggaata ctgggtggtc gaccccgaac
     1621 agcaactggt ctgggtctac cgtcgcggcg tgaaagccgg gaagaacggt gtcgagacgc
     1681 tgacttctga ttttcaggtc gtccaggcgt ttgatgtcga tgataaggcg atcgagatcg
     1741 tcgcacttcc gggcctgacc atcgacctga cccgcatttt tccgccgcga ccgaaggtcg
     1801 tgcgcgagcg accgcgctac tactcggtgt agaacaacga tggttcaaac ccagggtccg
     1861 tcgctggcag tttccccgca ttctgagttc gtttagaagg cttcttgcgc ggcgtttgat
     1921 tcctgtggct caagggtctt gcttatgcta tattcggcgg cacaccccgc ctgatgcggg
     1981 cagtcctcac accccgtctt tctaccggag atgatgattg atgaaaaacc ttctgcagga
     2041 actgaaatcg aagaaaaccg ccctgtgcgt ggtcgggcaa ggctacgtcg gcctgccgct
     2101 ggcgcatgcg ttcgcgaaga agttccgcgt gatcggcttc gacgtcaaca aagagaagat
     2161 tgctctgctg aagaaaggca tcgacccgac cggcgaagtt cctgaaggcg gtctgaaaga
     2221 ggtcaagatc gagtttaccg acgatccgtc gcgcattgcc gaggcgaagt tcatcgtcgt
     2281 ggcggttccc accccggtcg acaagaacaa gcgccctgat ctgtatccgt tggtcgcctc
     2341 gtcgacgacc gtcggcaaac atttacagaa gggttcggtg gtcgtctacg aatcgacggt
     2401 gtatcccggt gtcaccgaag acgattgctt gcctctgctt gaaaagcact ccggcctgaa
     2461 gtatggccgg gatttttcga tcggctacag ccccgagcgc atcaatcctg gcgacaagga
     2521 gcatcgtctc gaaacgatca ccaaggtggt ttctggctca gacgacgaaa cgctcgaact
     2581 ggtggcgcag gtctacgaaa ccatcatcaa ggcgggagtg catcgggctt cgagcatcaa
     2641 ggtggctgag gccgccaagg tgatcgagaa cacccagcgc gatctgaaca ttgcgctgat
     2701 gaacgagctg tcgatcatct ttcataaaat gggcattgat actcgagaag ttctcgccgc
     2761 cgctggtaca aaatggaact tcctgaagtt cttccctggc ctggttggcg gtcactgtat
     2821 cggcgtcgac ccatactacc tgaccttcaa agccgaagaa ctgggctatc accccgaagt
     2881 gattctggcc ggtcgtcgca tcaacgacgg catgggcaag tacgtcgccg agaacaccgt
     2941 gaaagagatg atccgggtcg gcaagcacgt gcgcggagcc cgggtgctga ttctcgggct
     3001 gaccttcaaa gagaatgttc ccgatctgcg caacacgcgc atcgtcgaca tcatagccga
     3061 gctcaaggaa tatggtgtcc actcgtatgt ccatgacccg ttggcagacc cgcacgaagc
     3121 cgaagaagaa tacagcctgc acttgtacac tgacctgaag aagattccac cggtagatgt
     3181 gatcattctc ggcgtcgccc acgatgaata ccgcgacctg tcgctgaaga agctcgtcgc
     3241 caaatgcagt cgcggcgaaa aagctcccgt cctcatcgac gtccgcggcc tctggaccca
     3301 ggacctcgcc cgcaaagccg gcttccacta ctggcgcctg taatcagaag gagatgaacc
     3361 cgtgacgacg aaaatgacca tgatctactg gaaatcgaaa aagttctggc tcgggaagat
     3421 cctggagcat cctgaaatca tgtcgcaggg cgaatcgatc gaggaacttg aaggaaattt
     3481 gaaagaagcg tttcgcctca tggcttttga tgatgttccc gagacgtttc aggtaaaaga
     3541 cattgcagta tgaagcgagg cgagctgatc cgtcagcttg aggcagcagg ctgtgtgctt
     3601 ctgcggcatg gt
//
LOCUS       DAORHD010000381         3608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28592_length_3608_cov_3.554461, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000381 DAORHD010000000
VERSION     DAORHD010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28592_length_3608_cov_3.554461"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="PKO06_09160"
     CDS             complement(<1..955)
                     /locus_tag="PKO06_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69853.1"
                     /translation="MANISAPALSQLKTALEAQVSGRRGAPSVPYLVPALWAPVGVEP
                     VGVEPQAVDPASWLLEHIRAIESRAMPGVRAEHSLNAQISGGTGGEWVGKSPIYNLFV
                     RLGTAFDHDGDGCLGGAKHDLTLNRHGVRESGTLLKALALLGHVHRLGARTIHLLPVT
                     AIGRDGNKGALGSPYAIKNAYRLEPTLADPLVALDVEVQFRAFIEACHLLGIRVICEF
                     VFRTASKDADWIAEHPDWFYWIDSTVPDRNPGETNLDRARTSYGNPIFDEETLKQVKQ
                     KVERGDFHELPPPPESYRRFFKLPPSSGQVRPHSVAAASSMP"
     gene            1146..1316
                     /locus_tag="PKO06_09165"
     CDS             1146..1316
                     /locus_tag="PKO06_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV69854.1"
                     /translation="MAIKVDRNKCTGCEACIGTCPVQVLEMKDGKCVYKDEGCIECGA
                     CVSVCPVEALTL"
     gene            complement(1395..2558)
                     /locus_tag="PKO06_09170"
     CDS             complement(1395..2558)
                     /locus_tag="PKO06_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69855.1"
                     /translation="MHKSVWRALFLGNLVVVGLFGPTIVATAEDPEVKIEEEKEAAKQ
                     AATEGRKQYEEALKLKRSGKWDDAIQMYEKAIRADRSILGDDDEGLMTALQKTYETKL
                     ASDSENLYLLEGLGYISAVGFSDFDKAIRYYQKVVELSKDEAVKERTGNLIERLRAQA
                     DLAKQMTADVSAQSREDRIKQWAELEKQDAAAAQAEKSQQREEKLSELYTKRDDLEAR
                     MPQIEDEIKNLQEDVDHNRRMYLNSNDRTYKRRQDRLEDDLESKKRELQRIKDDLVKT
                     NSDITKTAGEKLETAPAKPNPPTSPDATTPGGAPDPNLPPDPNQPVDPNNPPDPSAQP
                     QPGDPSLPPMQSPDFPPDPGAGSPPPADGSGAGTTPPPAGDASAPADASAPPA"
     gene            2825..>3608
                     /locus_tag="PKO06_09175"
     CDS             2825..>3608
                     /locus_tag="PKO06_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNV69856.1"
                     /translation="MSEKLKVLVADSIAEKGVQLLKSHSALDVTVNTKLTPEELVKTI
                     PAFHCIIVRSATKVTRPVIEAGKNLKLICRAGVGLDNVDQEAAKERKIEVRNTPACTT
                     ISVAELAFGLMICAARKVGPANQSMKDGKWDKKSFEGCELYGKTLGLIGIGRIGQAVA
                     ERAKAFGMQIIASDPFVKTSPLPYVTMTSKEDLVTKADFISLHIPFDKKAGPTLGEAE
                     FKTMKKGCILVNCARGGTVNEKALLAALNDGIVAGAAIDVWEK"
BASE COUNT          728 a         1071 c         1034 g          775 t
ORIGIN      
        1 tgggcatcga gctggcagcc gccacgctgt gggggcgcac ctgccccgaa ctgggcggca
       61 gcttgaaaaa ccgccgatac gattcgggag gcggaggcaa ctcatgaaag tcgccacgtt
      121 ccaccttctg tttcacctgt ttcaacgtct cttcatcgaa gatggggttt ccgtagctgg
      181 ttctggctct gtccaggttc gtttcgcccg gattccggtc agggacggtt gaatcgatcc
      241 aataaaacca gtcgggatgt tccgcgatcc agtcggcgtc tttcgaggcg gtgcgaaaca
      301 cgaattcgca gatgacgcgg ataccgagca gatggcaggc ttcgatgaag gcacggaact
      361 gcacttccac atccagagcg accagagggt ctgccagcgt cggctcgagt cgataggcgt
      421 tcttgatcgc atagggagac ccgagagctc ccttgttgcc gtcgcgaccg atcgcggtca
      481 ccggcaacag atgaattgtc ctggctccga gccgatgcac atgccccagc agagccagag
      541 cttttaacag cgtgcccgat tcgcgcactc catgacgatt caaggtcaaa tcgtgttttg
      601 cacctccgag acagccgtct ccatcatggt caaaagccgt accaagtcgg acaaacagat
      661 tgtagatggg tgattttccg acccattcgc ccccggtacc gcccgagatc tgcgcgttca
      721 atgagtgttc ggcacgaacg ccgggcatcg ccctggattc gatcgcccga atgtgttcga
      781 gcagccacga cgccggatca acagcctgcg gctcgacacc gacaggttcg acaccgacgg
      841 gcgcccacaa tgccggcacc aggtagggaa cggacggtgc gccccgacgg cccgaaacct
      901 gggcttcgag agcggttttc agttgtgaca atgctggggc tgagatattg gccatgacca
      961 cgtctccctt gtctatggtg tacagaaaat tgatctgcgg gattgtatca cagacaggga
     1021 atgggcagca ggatttcgag ctcagacgct gctgatgaga aaaaattcag gtctttactt
     1081 tcgcctcaga ttcctgtaca ctggcgccgc ctcgacacga ggtaaactac gcaaggagcg
     1141 agatcatggc gatcaaagtc gaccgcaaca agtgcaccgg ctgtgaagcc tgcatcggca
     1201 cctgcccggt ccaggtcctc gagatgaagg acgggaagtg cgtctacaag gacgaagggt
     1261 gcattgagtg cggagcatgc gtttcggttt gcccggttga agcactgacc ctgtaatcct
     1321 ctacaacaag cgccgctcag attcattctg agcggcgctt gttttttttc tgctgtcccc
     1381 ccggcgggat cccgttatgc cggcggagcg gaagcatccg cgggtgccga agcatcgcct
     1441 gccggaggcg gagtggtgcc cgctcctgaa ccgtcagcgg gcggcggaga accagcgccc
     1501 gggtcaggcg ggaagtcggg gctctgcatc ggcggcagag acggatcgcc cggctgaggt
     1561 tgtgccgaag ggtcgggcgg attgttcgga tcaaccggct gattcgggtc gggcgggaga
     1621 ttcggatcgg gcgctccacc cggcgtcgtg gcatcaggtg acgttggcgg attcggcttg
     1681 gccggggcgg tttcgagctt ctcgcccgcg gttttcgtga tatccgaatt ggtctttacc
     1741 agatcgtctt tgatccgttg cagctcccgc tttttgcttt ccagatcgtc ttccaatcga
     1801 tcctggcggc gcttgtaggt tctatcgttc gaattcagat acatgcgtcg attgtggtcg
     1861 acgtcttcct gcagattctt gatctcgtct tcgatctggg gcatgcgcgc ttcgagatcg
     1921 tcacgtttcg tatacaactc cgaaagtttc tcttcgcgct gctgactctt ctctgcctga
     1981 gcggccgccg catcctgctt ctccagttcc gcccactgct tgattcggtc ttcgcgtgac
     2041 tgcgccgaca catcagccgt catctgtttc gcgagatccg cctgagcgcg caggcgctcg
     2101 atcaggttgc cggtgcgctc tttgaccgct tcgtctttcg aaagttcgac gactttctgg
     2161 tagtagcgga tagctttgtc gaagtccgag aaccccacgg cggaaatgta gccgaggccc
     2221 tcgagcaggt acagattttc ggaatcactc gccagcttgg tttcgtacgt cttctgcagc
     2281 gcggtcatca gcccttcatc gtcatcgccg agaatactgc ggtcggcacg aatcgccttc
     2341 tcgtacatct gaatcgcgtc gtcccatttc ccgctgcgtt tcagcttgag agcctcttca
     2401 tactgtttgc ggccttccgt cgcggcctgc ttggcggctt ccttttcctc ttcgatcttg
     2461 acctcggggt cttcggcagt cgcgacgatc gtcgggccaa aaagcccgac aacgaccagg
     2521 tttcccagga acaatgcgcg ccacacgctt ttatgcattc cacacctctc cgccgtagtt
     2581 gggcttctca cacagcaccc ggcccgccgg ggctgaccgg tcagcgaaaa gcgacttccg
     2641 caaacagaac ctcggtggtt ccaagaaacg gcatgccaac caagcatatc atagccctct
     2701 cacgaccaca acaaatcgct gggaaactcg ctcgccggaa tcgggagctg ggttgacttc
     2761 acccgggcac ggtgctagag ttgcgttgtc ctcaataaaa accgcatcag gaaaggaaat
     2821 tgtcatgtcc gagaagctga aagttctggt tgccgactcg atcgccgaga agggtgtgca
     2881 actcctcaag agccattcgg ccctggatgt cacggtcaac accaagctga cccccgaaga
     2941 gctggtcaag accattcccg ctttccattg tattatcgtc cgttccgcga ccaaggtgac
     3001 tcgtcccgtc atcgaggcag gcaagaacct gaaactcatc tgccgtgccg gtgtgggtct
     3061 cgacaacgtc gatcaggaag ccgccaaaga gcggaagatt gaagttcgca atacgcctgc
     3121 ctgcaccacc atctcggtcg ccgaactcgc gttcggtctg atgatctgcg ccgcgcgcaa
     3181 agtcgggccg gccaaccaat cgatgaaaga cggcaagtgg gacaagaagt catttgaagg
     3241 ctgcgaactg tatggcaaga ccctcggtct catcggtatc ggtcgcatcg gtcaggcggt
     3301 cgccgaacgc gccaaggcct tcggcatgca gattatcgcc agtgatcctt tcgtcaaaac
     3361 cagcccgctg ccctatgtca ccatgacttc caaggaagac ctggtcacga aagcagattt
     3421 catcagcctc cacattccat tcgacaagaa agccggtccc acgctgggcg aagccgaatt
     3481 caagaccatg aaaaaaggct gcattctcgt caactgtgcc cgcggcggaa cggtcaatga
     3541 gaaagctctg ctggccgcct tgaacgacgg gatcgtggcc ggtgccgcca tcgacgtctg
     3601 ggaaaagg
//
LOCUS       DAORHD010000382         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28696_length_3600_cov_2.948096, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000382 DAORHD010000000
VERSION     DAORHD010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28696_length_3600_cov_2.948096"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PKO06_09180"
     CDS             complement(<1..974)
                     /locus_tag="PKO06_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV69857.1"
                     /translation="MKATRRGPLGTGILFAFFLLIMVVGCEKGTLGVKGGSISGHVLD
                     SRTLVGLSGVAVTASNGEGDEKDSKLTTSDSQGNYYFNDLRAGEWDLAFDKYGYEPVT
                     AEATGAAKVVVVNNENRQAPEVRMVQVIANQYITIRGTLKDARTGTPITLGTAQFSFG
                     THPILNNRLPTELQTGLSFPALAGDVDFRIAVTNYKPLTITGLNGAADIDLGVITLEP
                     QSYSIVGVWKDVPGWVFQEKARADIIAYAGNRIVATTTSTLDEQTFTLTGIPIGVSVT
                     IEAQILGYRMNGPIPVTPNGDFQGVIYQPVSLKNNFAQIYRDVRVILVG"
     gene            1160..2506
                     /locus_tag="PKO06_09185"
     CDS             1160..2506
                     /locus_tag="PKO06_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV69858.1"
                     /translation="MGNSLRATKARWLLPAILVMASIMFTGCEKGSLGVKNGSITGFV
                     LEETKNTPLTDVLVRIQHPSSLQKWETLTGANGQYTFNDMVPNSGNAVYTILAAEKFG
                     YRMATDTFAQNRLTVGLGDGGITVTVPVIQMYKLNNSVKGTLKGYPVDAITGTPLTNF
                     TVTQMTPSRSKTFEAATDFRDSGWQSLEGGEHQYKITCENYKPYITAGNEQTPTEANM
                     RVVIGAAPFDMGVVKLQPETVSINGTLRNLPGYVLDAMSGANPDFLIWAESGGKTVAT
                     VTPTLTAGAPVYTLANVPVTVGAVSVKCKLRGYDVITINPSVALPRQRPGTIIAGIDC
                     DFHNVEPIKRDLRIVLRGSQPEEGTEEVPGTPSSIDDNEIIRVYLKQGGKDLVPYVDA
                     VGHNQQAEAYFSGVITGYKLEVMAINTNRGYIMGEDKELTVLENGNTVFTFFLEVQ"
     gene            complement(2606..3160)
                     /locus_tag="PKO06_09190"
     CDS             complement(2606..3160)
                     /locus_tag="PKO06_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69859.1"
                     /translation="MADAKVEKAQLNEGMDLFKQGELGKAEIKFREFIKAFPESDLAD
                     NACYNLAKIYLKRGDKKNALTWLDFLLEHYPKSDAAYFAKDEKVEVMRELGIGPAETP
                     DELFFKAKKALGANKLEEAEKLLFSFIEKYPSEDELLDNVHYNLALIAKKRGNLERVK
                     QHVEIIMSKYPDSDAALYAEDLLG"
     gene            complement(3164..>3600)
                     /locus_tag="PKO06_09195"
     CDS             complement(3164..>3600)
                     /locus_tag="PKO06_09195"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV69860.1"
                     /translation="ETLDRLMPQMPRQVPRYLMGVGTPEDFFLAIERGVDMFDCVHPT
                     RIARHGAVFTRTGRIDLSAQKWQLDEGPIDPECLCYACRHFPRGYLRHLFKAQEILAL
                     RLASLHNITFMLDLMREIREHLAAGSFRAFRDRFLARYLGKE"
BASE COUNT          793 a         1079 c          976 g          752 t
ORIGIN      
        1 cccaccaaga tcacgcgcac atctcgatag atctgcgcga agttgttctt cagcgagacg
       61 ggctgataga tgaccccctg gaagtctcca ttgggtgtca cgggaatcgg cccattcata
      121 cggtatccca gaatctgcgc ctcgatggtc accgacacgc cgatcggaat tcccgtcagg
      181 gtgaatgtct gctcatcgag cgtggatgtc gtcgtcgcga caatgcgatt accggcatag
      241 gcaatgatgt ccgcgcgggc tttttcctga aacacccagc ccggcacatc cttccagaca
      301 ccgacgatac tgtacgattg cggttcgagt gtaatcacgc ccagatcgat gtcagcggcc
      361 ccattcaacc cggtaatcgt cagcggtttg taattggtga ccgcaatgcg gaagtcgacg
      421 tcaccagcca gcgcggggaa cgaaagtccc gtctgcagtt cagtcggcag gcgattgttc
      481 agaatggggt gcgtcccgaa cgagaactga gctgttccca gagtaatggg agtcccagtt
      541 cttgcatcct tgagtgtgcc tctgatcgta atgtactgat tcgcgatcac ctgcaccata
      601 cgaacttccg gcgcctggcg attctcgttg ttcaccacca caaccttggc agccccggtg
      661 gcttcagcgg tgaccggttc gtatccatac ttgtcgaacg ccagatccca ttcgcccgcc
      721 cgcaggtcat tgaagtagta gttcccctgc gaatcggacg tcgtcagctt cgagtctttc
      781 tcgtcgcctt cgccgttcga tgcggtgacc gccacgccgc tcaagccaac gagcgtgcgg
      841 ctgtcaagga cgtggcccga gatgctcccg cccttgaccc ctaatgttcc tttttcacat
      901 ccaaccacca tgatgagaag gaaaaaggca aagaggatcc cggttccaag cggtcccctt
      961 cttgttgcct tcatatgaca gcctccctcg tcgaacggat aaactacggt tcctccacac
     1021 tatcggcgtg ttcctccgaa aaattgagaa gttgcgcgga aacgttttct ccgaagattt
     1081 ctttaaaaat cagacgagtt tcgccgataa gctatccggg aaaaactttt tcccgcagaa
     1141 gcacgcaacg gaggcaacca tgggtaattc tctgcgagca accaaagcga ggtggttgtt
     1201 gccggcgata ctggtgatgg ccagtatcat gtttaccggc tgtgagaagg ggtcacttgg
     1261 agtcaagaac ggctcgatca cgggctttgt tcttgaagaa accaagaaca cccctttgac
     1321 cgacgtcctg gtgcgaattc agcacccgtc gagtcttcag aagtgggaaa cgttaaccgg
     1381 agccaacggc cagtacacgt tcaatgacat ggttcccaac agcgggaacg ccgtctacac
     1441 gattctggcc gcggaaaagt tcgggtatcg catggcgacg gataccttcg cgcagaatcg
     1501 actgacggtc ggtctgggcg acggcggtat caccgtcacc gttccggtta ttcaaatgta
     1561 caagttgaat aactcggtga aaggcacttt gaaggggtat ccggttgatg caattaccgg
     1621 aacgccgctg accaatttca ccgtgacgca gatgacgccc agtcgttcga agacgttcga
     1681 agccgcgacc gacttccgtg acagcggctg gcagagtctc gaaggtggcg aacaccagta
     1741 caagatcacc tgcgagaact acaaacctta catcacggcc ggcaacgagc agacccctac
     1801 cgaggcgaac atgcgtgtcg tcatcggggc cgcccccttc gacatgggcg tggtcaaact
     1861 gcagcctgag accgtgtcga tcaacggaac gctgcgcaac ctgcccggat acgtgctcga
     1921 tgccatgtcc ggcgccaacc ccgacttcct gatctgggcg gaatccggcg gcaaaaccgt
     1981 cgcgacggtt accccgacgc tcacggcggg cgctccggtc tacaccctgg cgaatgtccc
     2041 agtcaccgtc ggcgccgtct ccgtcaaatg caagctccgt ggctatgacg tgatcacgat
     2101 caacccgtcg gtcgctctgc cccgccagcg ccccggcacg atcatcgccg gtatcgattg
     2161 cgacttccac aacgtcgaac cgatcaagcg cgacctgcgc atcgttctgc gcggcagtca
     2221 gccagaagaa ggaacggaag aagttccggg taccccgagc tcgatcgatg acaacgagat
     2281 tatccgcgtc tacctgaagc agggcggcaa ggatctcgtg ccctatgtcg acgccgtggg
     2341 tcacaatcag caggctgaag cctacttcag cggcgtgatc accgggtaca agctcgaagt
     2401 catggcgatc aacaccaatc gcggttacat catgggtgaa gacaaggaac tgaccgtgct
     2461 cgaaaacggg aacaccgtgt tcaccttctt cctcgaagtg cagtaagcag agaaaccccc
     2521 gcagatacga acccccgggc cagtggcccg ggggttcgac ttttcaccag aaagtcagat
     2581 cgccgtcagc agcggcactc gatggttatc cgagcaggtc ctccgcatac agtgcggcat
     2641 cactgtcagg atacttcgac atgatgatct cgacgtgctg tttcacccgc tccagattcc
     2701 cacgcttctt cgcaatcaga gccaggttgt agtgcacgtt gtcaagcagc tcgtcttcag
     2761 atggatactt ctcgatgaac gagaacagca gcttctcggc ttcctcgagc ttgttcgccc
     2821 ccagcgcctt cttggccttg aaaaacagct cgtcaggggt ttcggccggg ccaatgccaa
     2881 gttcgcgcat cacttcgacc ttttcgtctt tggcgaaata ggccgcatca gacttcggat
     2941 agtgctcaag caggaagtcg agccaggtca acgcgttttt cttgtcgcca cgcttcagat
     3001 agatcttggc caggttgtaa caggcgttgt cggccagatc actctcggga aacgccttga
     3061 tgaattcgcg aaacttgatc tccgcctttc ccagttcgcc ctgcttgaag agatccattc
     3121 cttcgttcag ttgggctttt tctacctttg catcagccat gggttactcc tttccaagat
     3181 aacgggccaa aaatcggtca cgaaaggctc tgaagcttcc ggcggccaga tgctcccgaa
     3241 tctcccgcat cagatccagc ataaacgtta tattatgcag acttgccaga cgcaaggcaa
     3301 gaatctcctg cgccttgaac agatgccgca gatacccgcg cggaaagtga cggcaggcgt
     3361 agcacagaca ttccgggtcg atcggtccct cgtccagctg ccacttctgc gccgacagat
     3421 cgatgcggcc ggtgcgcgtg aacacggcgc cgtggcgagc gatgcgcgtg ggatgcacgc
     3481 agtcgaacat gtcgacccca cgctcgatcg ccaggaaaaa gtcttcgggg gtgccgacgc
     3541 ccatcagata gcgcggcacc tggcgcggca tctgcggcat cagtcggtcg agggtttcgg
//
LOCUS       DAORHD010000383         3600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28698_length_3600_cov_2.730324, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000383 DAORHD010000000
VERSION     DAORHD010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28698_length_3600_cov_2.730324"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="PKO06_09200"
     CDS             complement(<1..1970)
                     /locus_tag="PKO06_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV69861.1"
                     /translation="MSLHRMFADLPVSRKLTFVTVMSSALVLLLAIGFAMAMEWHGFH
                     LQFERELELFGAVIGENCVAALTFNDPRAAEDTLKSLRVHPQITRVEVYTASQTLFAS
                     FGFPRPKGSELLIPPLLASTVIRVSPHEMEVLVPIRLADQYMGTVVLNGSLDALIARQ
                     KALLHMGLLIFCASVALAFLLSAYVSHLISSPLLQLAETARAVSQTRNYGLRGPDPGT
                     REEIGTLAGCFNQMLDQIQSEIQKRELAEREQARLATAIDQSAETIIVTDPAGIIQYV
                     NPAFERISGYARHEVIGQNVFILRSGEQTPEFYQDLWAVLHEGRSWQGHFINRRKDGT
                     TYEEDVTISPVRSAQGEVTNYVSVHRDVTREIALKQQLLQAQKLEALGTLAGGVAHDF
                     NNILGAILGFADLARSDLPPKHIATESLQEIIFGARRAQELVRQILAFSRKLEQSRQP
                     MDLVRVVRNAIKFIRPSLPASIEIRTLFEPEIAPVLIDVTQINQILLNLASNASHAME
                     NQKGTLEIRVFEEVLDASRSEQLPGCKHGPHIVMAVSDTGSGIRPEVLPHLFEPFFTT
                     KSVGKGTGLGLSVVHGIVKEHGGDLRVQSKVGQGTTMFVYFPRYLDELPVETPEGEWT
                     LAGKSDLFKGKVLAVDDEPALVLYAKRVLEMVG"
     gene            complement(1974..2546)
                     /locus_tag="PKO06_09205"
     CDS             complement(1974..2546)
                     /locus_tag="PKO06_09205"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNV69862.1"
                     /translation="MSPGYRLPEMSSRNWRLCLVAAVIGLCLSVSEGMAVEKPTDLEV
                     KAAFVLNFSLFATWEHLATASEELRIGFFETQIEAAKAFVKVLDGKVVDRRPVRLIGM
                     KNPEQIEECHIVYFANNEMPRLAEILERASQNEILTVGETVEFLKIGGMLAFQVENNR
                     VRFLANPSQARKAGIQLSAKLLRLASLVMP"
     gene            complement(2570..>3600)
                     /locus_tag="PKO06_09210"
     CDS             complement(2570..>3600)
                     /locus_tag="PKO06_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV69863.1"
                     /translation="PYDFANQRDPFDRVVNMDFAGGRRTQMEVAMSGRSGRHHWAGGF
                     EISDVWKRYLFNWDRAVNVDKNFPQRTSGIFLEDEWKLRPNLAVSAGIRWDDMDDEGR
                     FANPRYALVWHPYPKSSLKIMHGNAFRLPNTYERYFVDPVTKTSEAVDAERTTTTEVI
                     FEREHHGGFSTHLSGYQYRLTNLLSQQLDPADDMLVYVGDPLVRGRALEIGVEKRWEP
                     SKWVRANVSWHRTWYANSGQNLVNSVSRLGRLHAMTPLLQHRFFAGLELLACSERQTV
                     LGTQAPAYVKTNLTLTRPMKHLGVDLSLSVYNLFDVKIYDGASTEHAMDVIRQDGRLF
                     RLRAEYSF"
BASE COUNT          818 a         1065 c          944 g          773 t
ORIGIN      
        1 ccaaccattt cgagcacacg tttcgcatag agcaccagcg cgggttcatc gtcgaccgcc
       61 aggactttcc ctttgaacag atcagacttt ccggcgaggg tccattcccc ttccggggtc
      121 tccaccggaa gttcatccag ataacgcggg aagtacacaa acatggttgt cccttgtccc
      181 accttgctct gcacccgcag atcaccgcca tgttccttca caattccgtg cacgaccgaa
      241 agaccgagac ctgttccctt cccgacgctt ttcgtcgtga aaaaaggttc gaacagatgc
      301 ggcagaacct cggggcgaat cccagaaccg gtgtcggaaa ccgccatgac aatgtgtggg
      361 ccgtgcttgc accccgggag ttgttctgaa cgactggcat caagaacttc ttcgaacact
      421 cgaatttcga gcgttccctt ctggttttcc atggcatgcg aggcattcga cgcgagattc
      481 aagagaatct gattgatctg cgtcacgtcg atcaggaccg gcgcaatctc gggctcaaag
      541 agggtacgaa tctcgatcga tgccggcaga ctgggtcgaa tgaacttgat cgcgttacga
      601 accacgcgca cgagatccat gggttgccgg ctctgttcca gttttcggct gaatgccaga
      661 atctggcgca ccagttcctg tgctcgccgc gccccgaaga tgatctcctg cagactttcc
      721 gtggcaatgt gtttcggtgg caggtcggaa cgcgccagat cagcgaatcc gagaatcgcg
      781 cccagaatgt tgttgaagtc atgagcgacg ccacccgcca gcgtccccag ggcttccagt
      841 ttctgggcct gcaacagctg ctgtttcagg gctatctccc tcgtgacgtc gcggtgaacc
      901 gacacgtagt tcgtgacttc gccctgcgcg gatcgaaccg gggaaatcgt gacatcttcc
      961 tcgtaggttg ttccatcctt gcggcgatta atgaagtgcc cctgccacga tcgtccttcg
     1021 tgaagtacgg cccagaggtc ctgatagaac tcaggtgtct gttccccgct tctcagaatg
     1081 aagacgttct ggccgatcac ctcgtgccgc gcatacccac tgatgcgctc aaacgccggg
     1141 ttcacatact gaatgatccc ggccggatcg gtaacgatga ttgtttcagc tgactgatcg
     1201 atcgccgtgg caagccgcgc ctgctcacgt tcagccagct cacgtttctg aatctcgctc
     1261 tggatctggt cgagcatctg attgaagcag cctgccagtg tgccaatttc ttcccgcgta
     1321 cccggatcag gtccgcgcag gccatagttt ctggtctgcg acacggctcg cgccgtctct
     1381 gccagctgca gaagcggtga tgagatcaag tgcgagacat aggccgacag gagaaacgca
     1441 agagcgacgc tcgcacaaaa gatcaacaac cccatatgta acagcgcttt ctgacgggca
     1501 atcagagcgt caagcgaacc gttcaatacg accgttccca tgtattgatc ggccagtcga
     1561 atgggaacca gcacttccat ttcatgtggg gagacacgaa taacggtact cgccagcaaa
     1621 ggcggaatga gcaactcaga ccccttcggt cgcggaaaac cgaacgaggc aaagagcgtc
     1681 tgagaggcgg tatacacctc gacccgcgtg atctgcgggt gtacccgcaa cgacttcaga
     1741 gtatcctcgg cggccctcgg atcattgaag gtcagagcgg ccacacagtt ttcgccgatg
     1801 accgccccaa acagctcgag ttcacgttca aactgcagat gaaagccatg ccattccatg
     1861 gccatggcaa aaccgatcgc gagcaacagg accagagcac tcgacatcac ggtcacgaac
     1921 gtcaacttcc gtgacacggg cagatcggcg aacatgcgat gcaaactcat gggttacggc
     1981 atcaccagcg acgccaggcg cagcagtttc gcactgagct gtatccctgc cttgcgcgcc
     2041 tgtgacgggt ttgcgagaaa tcgaacccgg ttgttctcga cctgaaacgc cagcatacca
     2101 ccaatcttca aaaactcgac ggtctcgcca acggtcaata tttcgttttg gcttgcgcgt
     2161 tccagaatct ctgcgagacg cggcatctcg ttgttcgcga aatacacgat atgacactct
     2221 tcgatctgtt ctggattctt catgccgata agcctgacgg gacgacgatc aacgactttt
     2281 ccatccagta ctttcacgaa tgctttcgcg gcttcgatct gggtttcgaa gaagccgata
     2341 cgcagttcct cgcttgccgt tgcaagatgc tcccacgtgg cgaacagaga gaaattcaga
     2401 acaaatgccg ccttgacttc aagatcggta ggcttctcta ccgccattcc ttcagagacg
     2461 gaaaggcaca acccaatcac tgccgcgaca agacacaatc gccagttgcg agacgacatc
     2521 tcgggcagtc ggtagccggg actcattgcg tgatgttcgt gcgtctgcat cagaatgaat
     2581 attccgccct cagtcggaag agccgaccat cctgtcgaat cacatccatg gcatgttccg
     2641 tcgaagcgcc atcgtagatt ttcacatcaa acaggttgta caccgaaagg gacagatcga
     2701 ctcccagatg cttcatcggg cgggtcaggg tcaggttggt cttcacatac gccggagcct
     2761 gtgtgcccag caccgtttgt cgttcactgc aggccagtag ttcgagaccc gcaaagaatc
     2821 gatgctgcag gagtggagtc atggcgtgca aacgtccgag tcgcgacacc gagttcacga
     2881 gattctgtcc cgagttcgca taccaggttc gatgccagga gacgttggct ctcacccact
     2941 tactcggctc ccagcgcttt tcgacgccga tctccagggc acgaccgcgc acgagtgggt
     3001 ctccgacata caccaacata tcgtcagcag ggtccaattg ctggctgagc agattcgtca
     3061 accggtactg atatcccgac agatgcgtcg aaaaaccgcc atgatgctca cgctcgaaaa
     3121 tgacttcggt ggtggtggtg cgttcagcat cgacggcttc agacgttttt gtcaccgggt
     3181 caacgaagta gcgttcatag gtattgggca gtcgaaacgc gttcccatgc atgatcttga
     3241 gagaactctt cgggtatgga tgccagacca gcgcatatct cgggttggca aagcgccctt
     3301 catcgtccat gtcgtcccag cgaattccgg cgctgacagc gagattcggg cgaagtttcc
     3361 attcatcttc cagaaagatg cccgatgtcc gctgcggaaa attcttatcg acattaaccg
     3421 cgcgatccca gttgaagagg tatcgtttcc agacatccga gatctcgaaa ccaccggccc
     3481 agtgatgacg accactgcgg ccggacatcg ccacttccat ctgggtgcgg cgacctccgg
     3541 cgaaatccat attcaccaca cgatcgaacg gatcacgctg gttcgcaaaa tcgtacggat
//
LOCUS       DAORHD010000384         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28768_length_3594_cov_3.762645, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000384 DAORHD010000000
VERSION     DAORHD010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28768_length_3594_cov_3.762645"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKO06_09215"
     CDS             complement(<1..505)
                     /locus_tag="PKO06_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69864.1"
                     /translation="MNRRQNNVLTTCLTGAALLFYVGIAGAVNLLTSATMLYLNKAQH
                     SLQTGNIAAARAFFEQARKAAPSDPKILKFEQDFKVAVQKKVAELKREAEFHLRNKNL
                     PKALESYRRILTCEPEHQEAQQKVNELKQDQLIINQYQRTGIVVPESTGRSHDTTIAQ
                     FAALPPGA"
     gene            734..1675
                     /gene="fba"
                     /locus_tag="PKO06_09220"
     CDS             734..1675
                     /gene="fba"
                     /locus_tag="PKO06_09220"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNV69865.1"
                     /translation="MPLVSTKDLFKKAYDGHYAIGAFNVNNMEIVQGIFEAAIAEKSP
                     LIIQVSAGARKYARHEYLIHLIQASIEMAKSSGIDLPVVLHLDHGDSFDICKKCIDGG
                     FSSVMIDASHHPFEENIKITKEVVEYAHAKGVTVEAELGRLAGVEDAVKVAAKDATYT
                     DPQQAKEFVTKTGCDSLAVAIGTSHGAYKFKGDASLDFKRLAEIEKLLPGYPLVLHGA
                     SSVPKELVDVCNKFGGKLSDSKGVPEEMITKASQMGVCKINVDTDLRLAMTGQIRKVF
                     AENPGEFDPRKYLGPARDAIREVVRHKMRIMGCAGKA"
     gene            1681..2190
                     /locus_tag="PKO06_09225"
     CDS             1681..2190
                     /locus_tag="PKO06_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69866.1"
                     /translation="MHSWVCRFSVLLLLISLLGCGSSGGGGSGTGDPIEAEVGETIDA
                     LVKDIESLDAQKVSENWLDMNGKCFRTNVATPETMPNLKDRLNAFFQKVKPQSLTFQI
                     KDRGIESLGENGAQMLGDLVVTYTPVGAEDPVTMPSEKLNLYLERVGKWGVISFGSSH
                     YPTSFPPQL"
     gene            2283..>3594
                     /locus_tag="PKO06_09230"
     CDS             2283..>3594
                     /locus_tag="PKO06_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69867.1"
                     /translation="MNRMQFTRLGSYPGFAFSLGKGALAWQDWMTDFLVASSMSFDGA
                     QMAIISFLSGQILHRAELSAEAAELLRAGSGYALFSQDFTPDVQFESLLVKAEPYDFT
                     GQFWQGVQHQMQGSYEQAVAAYQQAIAANPLLPRAHNLLGLCLRQLQRLEDAEQAYLQ
                     ETTISPDSPDAFANLGVLCVKTGRTDEARGHFERSLDRDQFYLNALLQLSRLLQSASD
                     TSAPLLKALHLRLLAGHADQPSVQELLQSAATAAEKTLPEYISSLQSTLDLDALRVIK
                     RLETLRQNGAMLALLSGYTHLLERIPPHPGAAAFLMNWVRQRLTTLAPRIPDFLKPLW
                     HAQTQVLYQRWPDLTSSQKASAPTDTPADGPTGALSADEFYELLLQEVILDGQVQPEE
                     VQLVGRAKNLLKIDQPTHDRLWLQVRQRRQASLQSDDGRAFDRRL"
BASE COUNT          773 a         1055 c          963 g          803 t
ORIGIN      
        1 tggcgccagg gggcagggcg gcaaattgcg cgatggtggt atcgtggctg cggcccgtcg
       61 attcagggac gacgatcccc gtgcgctggt actgattgat gatcagctga tcttgtttga
      121 gttcgttgac cttctgctgg gcctcttgat gctcaggctc gcaggtcaga atcctgcggt
      181 aggattcgag cgctttcggc agatttttgt tgcgcagatg aaactccgct tcgcgtttga
      241 gctctgcaac cttcttctgc accgcaacct taaaatcctg ttcgaacttg agaatcttgg
      301 gatcgctcgg tgccgccttc ctcgcttgct cgaaaaacgc gcgggctgcg gcaatgtttc
      361 ccgtctgcag ggagtgttgc gctttgttga gatacagcat ggtcgcactc gtcagaaggt
      421 ttaccgcgcc tgcgattccg acgtaaaaaa gcagagcggc cccagtcagg caggtagtga
      481 gaacgttgtt ttgacggcga ttcataccta ttctcattct accagaccga cagtgtacaa
      541 gcgatgaaat tcagctgagc cgatcacctg acgagcctgc tcattccgaa acattcgaag
      601 ggaagcaatc gttgcccgtt gttctcgcct gcttttgact ttcgtccgtg atctttgtta
      661 gactgacgca ccggtttcgg gcttcatttc gagctttcgt accggtcaac cgtgatgaag
      721 aaggagcaaa cacatgcctc tcgtcagtac caaggatctc ttcaaaaagg cctatgatgg
      781 tcattacgcc attggcgcat tcaacgtcaa caacatggaa atcgtgcagg gcatcttcga
      841 ggccgcgatt gccgagaaat ccccgctcat cattcaagtc tctgccggtg cccgcaaata
      901 cgcccggcac gagtatctga ttcatctgat tcaggcttcg atcgaaatgg ccaagtcgtc
      961 aggcatcgac ctcccggtcg tcctgcatct ggatcacggc gacagttttg acatctgcaa
     1021 gaagtgcatc gatggcggct tcagctcggt catgatcgat gccagccacc atccgttcga
     1081 agaaaacatc aagatcacca aagaggtggt cgagtatgcg cacgccaagg gcgtcacggt
     1141 cgaagccgaa ctcggtcgtc tggccggagt cgaagatgcc gtcaaggtgg ccgccaaaga
     1201 cgcgacgtat accgatccgc aacaggccaa ggaatttgtg accaagaccg ggtgcgactc
     1261 actggccgtt gccatcggca ccagtcacgg agcgtacaag ttcaagggcg atgcttctct
     1321 tgatttcaag cgacttgccg agatcgaaaa actgctgccc gggtatccgc tggttctgca
     1381 tggcgccagc tccgtgccga aagaactggt cgatgtctgc aacaaattcg gcggcaaact
     1441 ttctgactcg aagggcgttc ccgaagagat gatcaccaaa gcgtcccaga tgggcgtctg
     1501 caagatcaac gtcgacaccg atctgcgctt ggccatgacc ggtcagatcc gcaaagtctt
     1561 cgccgaaaac cccggcgaat tcgacccgcg caagtatctg ggcccggccc gcgacgcgat
     1621 tcgcgaagtc gtgcgccaca aaatgcgcat catgggctgc gccggcaagg cctgattcgc
     1681 atgcattcgt gggtctgccg gttctcggtt ctgctgcttc tgatctcgct gctcggctgc
     1741 ggctcgagcg gcggtggcgg ctcgggaacc ggcgacccca tcgaagccga agtcggtgag
     1801 accattgacg cgttggtgaa ggatattgaa agcctggacg cacaaaaggt gagcgagaac
     1861 tggctcgaca tgaatggcaa gtgtttccgc acgaatgtcg ccactccaga aacgatgccc
     1921 aacctgaaag acagactcaa cgccttcttc cagaaagtga aacctcagag cctcaccttt
     1981 cagataaaag atcgcgggat cgagtcgctc ggagaaaatg gtgctcaaat gctgggtgac
     2041 ctcgtcgtga cgtatacccc cgtgggtgcc gaagatccag tgaccatgcc gagtgagaag
     2101 ttaaacctgt atctggagcg agttggaaaa tggggagtca tttctttcgg ctcatctcac
     2161 tatcccacct cgtttccgcc gcagttgtag gcacattttc cggcgttcat tttccttttc
     2221 ccctgacggg aaaaaccgta taatgtcggc gaaacctttc gtctcagggg cagcacttca
     2281 ccatgaaccg gatgcaattc acccgacttg gcagttatcc cggctttgcc ttttccttgg
     2341 gcaagggagc tctcgcctgg caggattgga tgaccgactt tctggtcgct tcatcgatga
     2401 gtttcgatgg ggcgcagatg gcaatcattt cgtttctttc aggtcagata ctgcaccgcg
     2461 cggaactgag tgctgaagcg gccgaacttc ttcgcgccgg cagcggctat gccctcttct
     2521 cacaggattt cactccggat gtgcaattcg aaagtctcct agtcaaagcc gagccctacg
     2581 acttcaccgg acagttctgg cagggcgttc agcatcagat gcagggaagt tatgagcagg
     2641 ccgtcgccgc ctatcaacag gcgatcgccg ctaaccctct gctaccgcgg gcgcacaacc
     2701 tgcttggctt gtgtctgcgt cagttgcaac gtctggaaga cgcagagcag gcctatctcc
     2761 aagagacgac gatctcgccc gactctcctg atgcttttgc caatctgggg gttctgtgcg
     2821 tcaaaaccgg tcgcaccgac gaagcccgcg gccatttcga acgatccctt gaccgcgatc
     2881 agttctatct gaacgccctc ctgcagctca gtcgtctgct tcagagtgcc agcgacactt
     2941 ccgccccgct tctcaaagcc ctgcatttgc gtctgctcgc aggtcatgcc gatcagccgt
     3001 cggtacagga actgctgcag agtgccgcca ctgctgctga aaaaaccctg cccgagtata
     3061 tttcttcact gcaatcgact ctcgatcttg acgctcttcg ggtcatcaag cgcctcgaga
     3121 ccctgcgtca gaatggggct atgctcgcgc tcctcagcgg gtatacccat ctcctcgaac
     3181 gcataccgcc acatcccggt gccgcggctt ttctcatgaa ctgggtgcga cagcggctga
     3241 ccacccttgc tccacgcatt cccgattttc tcaagccact ctggcacgca caaactcagg
     3301 ttttgtacca gcgatggcca gatctcacca gctcgcaaaa agcatcagct ccgacagata
     3361 ctcccgccga tggaccgaca ggggctcttt cagccgacga attctacgaa cttctgctgc
     3421 aggaagtcat tctcgacggt caggtgcaac ccgaagaagt acaactcgtc ggacgcgcca
     3481 aaaatctatt gaaaatcgat caaccgactc acgatcgact ctggctgcag gtacgccaac
     3541 gtcggcaagc cagcctgcag tctgatgacg ggcgcgcctt cgatcgacgg ctct
//
LOCUS       DAORHD010000385         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28795_length_3592_cov_3.704835, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000385 DAORHD010000000
VERSION     DAORHD010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28795_length_3592_cov_3.704835"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2662
                     /locus_tag="PKO06_09235"
     CDS             <1..2662
                     /locus_tag="PKO06_09235"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV69868.1"
                     /translation="SSNYSRVDWADLICRQLTEPVDFVAQTHVMREWRCGSFLEIGPG
                     RALQKFVGATLSGQPHRVLQLQSELLPTESERPVVNAELNFEANPLLRLLQKAIARVT
                     GYQLERITIEDRFQDDLGIDSLKTAEIILEVAKESGKRLDPAASMVECTDVRSAVEML
                     RCSLRTEIDPVKDDRSPSAFVRGRTCWKKRPIRNSIASNIRSASLDASGLRDRVEISV
                     EQIVRRSVDAAGCARQLRDRQKPLFILRADAPIVDWPQGAVGEKAALCVENLARCIEW
                     FQEFLREMTIPDLEMFLITSAATPPAVLGLSAFLASVRREYPAVFVKHLQIMEKTDLD
                     NQGWLLNLIDQERIDPFDFVVRYENGCRLVPELLPIPAKRAARVREVVVAFGGARGIT
                     FSLLTRILARGSHVYLLGRSPVGEVTVATNLETLRCRGLNVTYRSLDATDFLSVRRFL
                     DDVRRRRHRIDLVINGTGMEVSRPLARKTAQDIRAELRGKILPAIHIQAACAAVRPRR
                     FVQFSSVVARMGNVGQTVYSCANGVIDALVEDFNRSRSDIQAWAIDWPPWDGVGMTTR
                     VAIHDSLRARGISLLNEEQAAALFLQDLNEGMEGVCSYYDPDDQAVFQFPLRRIERLR
                     SLLGVPGSGRFFEKRFSLDSDRYLQDHRLDGQMLVPAAVAMCMIYWFALLQNPEVNTL
                     EAFEVVHPMVVGPNGLAVRLSREASGKGLEIASLARHAVAIPCACPTGDTPLVVEQQH
                     GAKVCGRLEEPPEDLYKKRLFHGPTFQLLHRVGTTSAGRRCAEIEWSRLVPLCGVEAI
                     DRSIVAIDAAFQIVAFEAICHGIGKMLPVRVESTTWVRESRWDRLYIDVSRFEILDDL
                     IRADVSACDDQGALCLRFEGIELRVMKSSV"
     gene            complement(2716..3162)
                     /locus_tag="PKO06_09240"
     CDS             complement(2716..3162)
                     /locus_tag="PKO06_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69869.1"
                     /translation="MRCRFLIIGLVLLVSFVASVTAKPPPPPPPPSITFTFINDTDHT
                     VKISSSVLCMYTPSSVSVPGLTITTTGERTITNQFIDVEKELPPGGRHTETRTYEFYY
                     KISGAAGSVYWNGRTERQDSNPGAQVKTVTVRAISSAPWVQTTATK"
     gene            complement(3402..>3592)
                     /locus_tag="PKO06_09245"
     CDS             complement(3402..>3592)
                     /locus_tag="PKO06_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69870.1"
                     /translation="KREVNGFFNSTDALKELEKLLGAEKYAALKTKALLKPYQAKTDP
                     AAQSAMYWPEDIEKAAEQ"
BASE COUNT          735 a          928 c         1069 g          860 t
ORIGIN      
        1 ttcttcgaac tattcgcgcg ttgattgggc tgacctgatc tgtcggcaat tgaccgaacc
       61 agtcgacttt gtggctcaaa cgcatgtgat gagggaatgg cgctgcggtt cgtttctgga
      121 gatcggtccc gggagggcgc tgcagaagtt tgtcggcgcg acgttgtccg ggcagcctca
      181 ccgcgttctt cagttgcaga gcgaacttct cccgaccgaa agcgaacgtc ccgtggtgaa
      241 cgccgagctg aattttgagg ccaatcctct gttgcgactg ctgcagaagg cgattgctcg
      301 tgtcactgga tatcaactcg aacgcatcac catcgaggat cggtttcagg atgacctggg
      361 tatcgattcg ctcaaaacgg cggagattat tcttgaagtg gcgaaagagt cgggaaaacg
      421 acttgatccg gctgcttcca tggtcgagtg caccgatgtg cgatctgccg tcgagatgct
      481 gcggtgcagt ttacgcacag aaatcgaccc ggtcaaagat gatcgatccc cgtctgcgtt
      541 tgtacgaggt cgaacgtgct ggaaaaagcg accgatacga aacagcatcg cgtcgaatat
      601 ccgatcagcg tctcttgatg catctgggtt gcgagatcgc gtcgagatca gtgtcgagca
      661 gatcgtgcgc cgaagtgtcg atgccgcagg ctgtgcgcga cagttacgtg atcgtcagaa
      721 accgttgttc attctgcgtg ccgacgcacc gatcgtcgac tggccacagg gcgcagtggg
      781 ggaaaaagcg gcgctgtgcg tggaaaacct ggctcggtgt atcgagtggt ttcaggagtt
      841 tctccgagag atgacgatcc cggacctgga gatgtttctc atcacttctg ccgcgactcc
      901 accggctgtt ctgggtttgt cagcattcct ggcgagcgtc cggcgtgaat accccgcggt
      961 gttcgtcaag catctgcaga tcatggaaaa gactgatctg gacaatcagg gatggttgtt
     1021 gaacctcatc gatcaggagc gcatcgatcc gtttgatttc gtggtgcgct atgagaatgg
     1081 ctgccgcctg gttcctgaac ttctgccgat acctgcgaaa cgtgccgcac gcgtgcgtga
     1141 ggttgtcgtc gcctttggcg gagccagggg catcaccttt tccctgctca cacgaatact
     1201 cgcacggggg agtcacgtgt atctgttagg tcgctctccc gttggcgagg tgacggtcgc
     1261 gactaacctc gagacattgc gatgccgagg gctcaacgtg acctatcggt cgctcgacgc
     1321 gactgatttt ctgtcggtgc ggcgatttct tgacgacgtt cgccgccgcc gtcatcgtat
     1381 cgatctcgtg atcaacggca cgggaatgga agtcagccgg ccgctcgcac gcaagacagc
     1441 acaggacatc cgtgccgaac tgcgtggcaa aatcctgccg gcaatacata ttcaggctgc
     1501 ctgtgccgct gttcgacctc gtcgattcgt acagttttct tctgttgtgg cccgcatggg
     1561 aaatgtcggt cagacagtgt actcttgtgc caatggggtg attgacgccc tggtggaaga
     1621 cttcaatcgc tccaggtccg acatacaggc ttgggccatc gactggcccc cgtgggatgg
     1681 ggtgggtatg acgacccgtg tcgccattca cgacagcctg cgtgcccgag gcatttctct
     1741 ccttaatgag gaacaggcag cagctctctt tttgcaggac ctcaacgaag gaatggaggg
     1801 agtctgcagt tactatgacc cggacgatca ggcagtcttc cagtttccgc tccgacgaat
     1861 cgaacgtctg cgatcactgc tgggtgttcc tggttctggc cgtttcttcg aaaagcggtt
     1921 ttcgctggat tccgatcgtt atcttcagga tcatcgactc gatggtcaga tgctggttcc
     1981 ggcggcggtt gccatgtgca tgatctactg gttcgccctt ctgcagaacc cggaagtgaa
     2041 cacgctcgaa gcgttcgagg tggttcatcc gatggtcgtg ggtcccaatg gcttggctgt
     2101 cagattatcg cgagaagcat ccgggaaagg gcttgaaatc gcctcgctcg cacgacatgc
     2161 ggtcgcgatc ccgtgcgcct gccccacggg agacacaccg cttgtcgtgg aacaacagca
     2221 tggcgcaaaa gtatgcggca ggcttgagga gccgccagaa gacttgtaca aaaaacggct
     2281 ctttcatggc ccgacctttc agctgttgca tcgagtcggg acaacctctg ccgggaggcg
     2341 gtgcgcagaa atcgaatggt ctcgacttgt acctttatgc ggggtcgaag ccatcgatcg
     2401 atcgatagtc gctatcgatg ctgcatttca gattgtggca ttcgaggcga tctgtcacgg
     2461 gatcggaaaa atgttgccgg tgcgtgttga atctacgacc tgggtgagag agagtcgttg
     2521 ggatcgtctg tacatcgatg tgtcgcgatt tgagatcctg gacgatctca ttcgtgccga
     2581 cgtgagtgcc tgcgatgatc agggtgctct atgtctgcgt ttcgaaggaa ttgagcttcg
     2641 cgtcatgaaa tcgagtgtgt gaagacccgg tttcggtcgc ggagaaccac agacaggcgg
     2701 accagtgttc ccagattact tggttgcggt tgtctgaacc cacggagcgg aactgatggc
     2761 gcgaacggtc acggttttga cctgagcgcc ggggttgctg tcctgacgct cggttctgcc
     2821 gttccaatag actgatccgg cggcacccga gatcttgtaa tagaattcat aggtgcgggt
     2881 ttcggtgtgt ctgccgcccg gcgggagttc cttttcgacg tcgataaact ggttggtaat
     2941 ggttctctcg ccggttgtcg tgatagtcag cccaggtacg ctgactgaag acggggtata
     3001 catgcacagc accgacgaac tgatttttac cgtatgatct gtgtcattga tgaaggtgaa
     3061 ggtaatggac ggcggcggtg gcggcggcgg tggttttgca gtaaccgagg ccacaaaact
     3121 gacaagcagc acaagaccaa tgatcagaaa tcgacaacgc ataattcccc ctcacagaca
     3181 tcaattgtac cggggcaacc ggtttaacgt actttattcg agtgtgtcga catggtgtct
     3241 caatcgtttt caacgactga tcagcaccct cgaaatcaat acgtcaatca taacaaacgg
     3301 tgccaagaat gacaagtttc gtttggctca ggtcctagtc gaacaaaaaa caaccccgcg
     3361 aatcgtcaga ttcgcggggt tgtcgcagac aagaattcga attactgttc ggcggccttt
     3421 tcgatatctt cgggccagta catggccgac tgggctgccg gatcggtctt ggcctggtaa
     3481 ggcttcagca gcgctttggt cttgagggcg gcatactttt ccgcgccgag cagtttctcg
     3541 agctctttca gagcatcggt gctattgaaa aagccgttca cttcgcgctt gg
//
LOCUS       DAORHD010000386         3592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28797_length_3592_cov_3.468759, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000386 DAORHD010000000
VERSION     DAORHD010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28797_length_3592_cov_3.468759"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="PKO06_09250"
     CDS             <1..1899
                     /locus_tag="PKO06_09250"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV69871.1"
                     /translation="RRVLTQSYQSGVELVFAEAEALKVRVEQRAVELTTRSRAAYHTS
                     MVFLIIVFVVGVIVLTVYPARVSGDLQRLSDQIGSYQIGTALDAAAPIHTRELRDLRG
                     AFCELADRLTGQVGVIGRYTNAIEHMGDVFATLHRHSEEPGSGEQALRESVYRALEQL
                     SVIEGLSLAKVMVRRRRGGEVVLSQLGDEYVAPDFVESGECREYLNKTPAEAKAALAD
                     VRDSMPYDEGISGWLMNYWDAPEGRVASFDGHRDLIANRDFLQRKMVIPVIGEQQQLA
                     KRKFERGLMGAAFGVKIPWLADQAARRRRGGIKDPGPIEREFVPSDEGMIGILFLYFA
                     DRETRISEQERLFAQIIGGQIAQLIHAYLMLDQQKELQKLKYHLQMAAEIQEHLLPAK
                     NRVPRHPHVQISVVCKPAGDVGGDYYDFPFPPFQIGGRADPPPDTIHVIIADASGKNI
                     PAALLMTILKTAVASMDIGTMRPHEVLMTANQIINKCITSDRFITALYLILHTQTGIV
                     EVASAGHCLPIHVSPKGAEPRIHQLKARSLMPLGIMEDTRYETDRFQLHPGDGLFLYT
                     DGVIEARNSGPNPEEFGMERLKKFLLRDRPKDFAAALLAEIEQFRGDAAQHDDITAVL
                     VEWSGGKS"
     gene            1896..2579
                     /locus_tag="PKO06_09255"
     CDS             1896..2579
                     /locus_tag="PKO06_09255"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV69872.1"
                     /translation="MNEQPNPNRDLSAETQSRTYWDKTWSKFLKYQGMIAPSGKLIQL
                     LIGMVERGGTVLDLGCGEGRNTIYMARVGFRVVGLDLSQKGVRVLANNLFEEEVRATA
                     LVGDTRRLPFALGSFDAILAHNLFDHVDAETSKIGLQECYRVLKPGGYLLLTWDSLPQ
                     HLDRKQIVTCDDGSHVYTTGSHKGMLLRPWSAGELAATVEQGWDVIKDELTPRQSKVL
                     LWRKKLQHG"
     gene            complement(2650..3429)
                     /locus_tag="PKO06_09260"
     CDS             complement(2650..3429)
                     /locus_tag="PKO06_09260"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNV69873.1"
                     /translation="MKSRWEKSSDTSVTGPESRTPDSEARLLADSLKRFSDEIVGIFA
                     RTQTQNAKLRDELEVARDIAFPAVLALSELMEGRDNVTYGHSRRVARICVQVVDHLRW
                     PVERVKLMNMTCLLHDIGKVIIPDAILNKEGKFTPLEYETMKLHSSTGSRIVQRLGFL
                     GEEAETWILHHHERWDGEGYPDGLTGDQIPLGARILCMADTYDALTTARRYRQKMEHW
                     VALDEIVKCGGKQFDPDLAMSFLDALKEPPELEPTKNLIGS"
BASE COUNT          749 a          995 c         1068 g          780 t
ORIGIN      
        1 cgtcgtgtct tgacgcagag ctatcagagc ggagtcgagc tggtgtttgc cgaagccgaa
       61 gcgctgaaag tgcgtgtgga gcaacgggcg gtcgaactga ccacgcgcag tcgcgccgcg
      121 tatcatacga gtatggtgtt cctgatcatc gtgttcgtgg tcggcgtcat cgtgctgacg
      181 gtatatccgg ctcgtgtctc gggtgatctg cagaggctga gcgatcagat cggaagctac
      241 cagattggta ccgcactcga tgccgctgca ccgattcata cccgcgaatt gcgtgatctg
      301 cgcggagcgt tctgcgagtt ggccgatcga ctcacgggtc aggtcggggt catcggacgg
      361 tataccaacg ccatcgagca tatgggagac gtatttgcca cgctgcatcg gcactcggaa
      421 gaaccggggt cgggggaaca ggcgctacgg gaatcggtct accgggcact tgagcagctg
      481 tcggtcattg aggggttgag tctggccaag gtcatggtcc ggcggcgccg gggcggtgag
      541 gttgttctct cgcagctggg agacgaatac gtcgcgcccg attttgtcga gtcaggagaa
      601 tgccgcgagt atctgaacaa aactcccgct gaggcgaaag ccgcgctggc agatgtgcgc
      661 gattccatgc cttatgatga aggaatttcg ggctggctga tgaactactg ggatgctccg
      721 gaagggcgcg tcgcgagttt cgacggccat cgcgacctga ttgccaaccg ggattttctc
      781 cagcgcaaga tggtgattcc ggtcatcggg gaacagcagc agctggccaa gcgcaagttc
      841 gaacgtggtt tgatgggggc cgcgttcggc gtcaagattc cgtggctggc agatcaggcg
      901 gcccgtcgcc gccgtggcgg catcaaggat cctggcccga ttgaacgcga gtttgtgccc
      961 agtgacgagg gaatgatcgg tattctcttt ctctattttg ccgatcgtga gactcggatc
     1021 tcggaacagg aacgactgtt tgcgcagatc atcggtggac agatcgcgca gctgattcat
     1081 gcctatctga tgctcgatca gcagaaagag ctccagaagc tgaagtatca cctgcagatg
     1141 gccgccgaga ttcaggaaca cctgctgccg gccaagaacc gggtgccgcg tcatccgcac
     1201 gtgcagattt cggtcgtctg caagcctgcc ggcgatgtcg gtggtgacta ttacgacttt
     1261 ccgtttccgc ccttccagat tggcggacgg gctgacccgc cgcccgacac gattcatgtc
     1321 atcattgccg atgcctcggg caagaatatt cccgccgcac ttctgatgac cattctcaag
     1381 acggcggttg cctcaatgga catcggcacc atgcgccctc acgaggtgct gatgacggcc
     1441 aatcagatca tcaataagtg tatcacctct gatcgcttca ttacggcact gtatctcatt
     1501 ctgcacacgc agaccggcat tgtcgaagtc gcgtcggctg gtcactgtct gccgattcat
     1561 gtttctccga aaggcgcaga accacgcatt catcagctca aggcccgcag tctgatgccc
     1621 ctcggcatca tggaagacac gcgttatgaa acggatcggt ttcagctgca cccgggagac
     1681 ggactctttc tctacaccga tggcgttatc gaggcgcgaa acagcggccc gaacccggaa
     1741 gaattcggca tggaacgcct gaaaaaattc ctgctgcgag atcgacccaa agacttcgcg
     1801 gccgctctgc tggctgaaat cgaacagttt cgcggtgatg ccgcccagca tgacgatatc
     1861 acggcggtac tggtagaatg gtcagggggg aagtcatgaa cgagcaaccg aacccgaacc
     1921 gcgacttgag cgcggaaaca cagtcacgga cgtattggga caaaacctgg tcgaagtttc
     1981 tgaagtacca gggaatgatc gcaccgtctg gaaaactgat tcaactgctg atcggcatgg
     2041 tcgagcgtgg cggaaccgtt ctcgatctcg gatgcgggga aggccgcaat accatttaca
     2101 tggcacgcgt cggctttcgc gtcgtcgggc tcgacctgtc ccagaagggc gtgcgcgttc
     2161 tggccaacaa tctgtttgaa gaggaagtgc gcgccacggc tctcgttggc gacacacgcc
     2221 gtctgccctt tgccctcggc agtttcgacg cgattctggc tcacaacctg ttcgatcacg
     2281 tggatgccga gacttcgaaa atcgggctgc aggaatgtta ccgcgtgctc aagccgggcg
     2341 gttacctgtt gctgacctgg gattcgctac cgcaacatct cgatcgcaag cagatcgtga
     2401 cttgtgatga cggctcgcat gtgtatacca ccggttcaca caagggcatg ctgttgcgcc
     2461 cctggtcggc gggagaactc gccgcgactg tcgagcaggg ctgggatgtc atcaaagacg
     2521 aactgactcc gcgtcagagc aaagttctgc tgtggcggaa aaaactgcaa cacggctgaa
     2581 tactgtctgc cgatttcccg ctacgagaag ctcttcgtga gataatcgac ttgtactatt
     2641 gacgcggaat cacgagccga tgaggttttt cgtgggctca agttccggcg gttctttcag
     2701 cgcatcgagg aaggacatcg cgagatcggg atcgaactgc ttaccgccgc atttcacgat
     2761 ttcgtccagc gcgacccagt gctccatttt ctgtcgataa cgtcgggcgg tcgttagagc
     2821 atcatacgtg tcagccatgc agagaatgcg cgcgccaagc ggaatctgat cgccggtaag
     2881 cccgtcgggg tatccctcgc catcccagcg ttcgtgatga tgcaggatcc aggtctcggc
     2941 ttcttcgccg agaaagccca ggcgctgcac gatacggctt cctgtgctgc tgtgcagttt
     3001 cattgtctcg tactcgagcg gggtgaactt cccttccttg ttcagaatcg cgtcgggaat
     3061 gatcaccttg ccgatgtcgt gcaacagaca ggtcatgttc atcagtttca cccgctctac
     3121 aggccaacgc agatggtcga caacttgtac gcagatgcgg gcgacacgac gactgtgacc
     3181 gtaggtgaca ttgtcgcgtc cctccatgag ttctgaaaga gccagcacgg cgggaaaggc
     3241 gatatcgcga gccacttcga gttcatcacg cagtttcgca ttctgtgtct gcgttcgggc
     3301 gaaaatgcca acgatttcat cactgaatcg tttcaacgag tcggccagca gtcgtgcttc
     3361 actatccgga gtccttgatt ccgggccggt gaccgaggtg tcggatgatt tttcccagcg
     3421 gcttttcatg gaaggtttgg cattcccgcg tttgtttgct agtataccat cgaagcaacg
     3481 acaacgcacg gggcgccgat catgcaggtg acacaaacca ttacgattca gattcatcct
     3541 gcccatcacg aagcgggcaa ggcgatcgac catctggctc agacgctcaa ag
//
LOCUS       DAORHD010000387         3591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28809_length_3591_cov_2.799491, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000387 DAORHD010000000
VERSION     DAORHD010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28809_length_3591_cov_2.799491"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..3146)
                     /locus_tag="PKO06_09265"
     CDS             complement(153..3146)
                     /locus_tag="PKO06_09265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV69874.1"
                     /translation="MNPFARWLIAIVIIWAIPAFFTWYGLAQYELLTRERLLEAMYEN
                     QKQVLEERSTLLDSDLFLHAAAQNLRTRLRLLGLSSHDQHQRQTAVVQDWANSFPAGF
                     LEWYLFNASGTIWLASGTIPSESRQTVIHSLRQTGASLYLTAVPETVRTSWEHAGYAL
                     PRLWNRADIPVLLHQLKGHGQGTHWGYWWEPSSPMDYGGFLIARENAMTREQRISWLS
                     AHDEPGTFSGFFTHTTSVQGNMPGAMDEFSACLFRQIELGHERALHGAYVTTCLWATR
                     DHALWIAQPQPVAGVPWRVFLAGYGLLSVLFLSRSYEMLVLDGHRPVRLSWKLAGLFT
                     LILGFPLAGVYLLGHFLVEEKRNELVQDCRRESLDRLRQLDQGYAQFVACECLRYRAR
                     IRRLEAFRHEPEALKKQVRAWFGSGGFDNHYMVSSASVFLAFEFNTSRALRRRLARIP
                     QSEKERVFLGWITAGLIPGEADLRRFRGEPEPDCYPNQKEDLIKQATARMTLSALKQH
                     DSDAGLEADKPDDGAVNALDVMIGDSNFDMVRVLRQKTTGFFFAGTLLDGNYYLFDII
                     RGPEGHAWYGILLGAEVGTIEGPYLEALSAHLNRARTATTFRFISTHDPYSPYYPDYA
                     HAPMYQKLTERLRRGRRNVLFQRMELEGEPIEVVGMIGAKLMKYALYATTPVSAIEHR
                     VLRFRRQVFLQLAVAAVLGSLLAMMIVRLLLVPVRELERGLEIIRTKRYESSIPVYSN
                     DELGQLCHAFNRAILHLSDMEVASTVQKRLLPQESLRVGSFEWRGTNLMTQAVGGDYY
                     DAISISSRQVAFALGDVSGHGVSAALVTAMAKAGFSILCPLFADDPKRVLELINHAFV
                     DILQRKKMMTMFLAILDIDTGVVTFANAGQCSPILAGADGTCRYLRADSRPLGITKRF
                     GYENRTITDFPAAILLYSDGIVEAQTREGEMVGYERFLSLTEESLQKQIPDPLDSIRE
                     RVATFLSDKGFDDDVTLLLLRRQSS"
     gene            complement(3143..>3591)
                     /locus_tag="PKO06_09270"
     CDS             complement(3143..>3591)
                     /locus_tag="PKO06_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69875.1"
                     /translation="GSVISPALIVQQLQAYVNRHLAPQQPFSWCAGRIEMDTGSATLF
                     HTGGFGLYSSSSNTGLCRICLQAGTEETPANLELLAVLSPALLQTSVASGESVVLQLL
                     GSLTEHQFDQPECARRLFAHTPFATMTGDQAESQTLLLLTRGGHQP"
BASE COUNT          833 a          997 c         1040 g          721 t
ORIGIN      
        1 cacgccgaaa tcgactccac aatgatgagg cgaagaggtc agggcaatga ggatcatgct
       61 tgcccagagg aaggtgctgg tatcgatacc atgagaaccg gacgtttgac cagcctgaga
      121 aagaggaatt ccgacggtgg gacctctcgg gttcaggatg attgccgacg aagaaggagg
      181 agcgtgacat cgtcatcgaa ccctttgtca gacaagaagg tggcaacccg ttcgcgtatc
      241 gagtcgagtg ggtcgggtat ctgtttctgc aggctctctt cggtcaatga aagaaaacgt
      301 tcgtagccca ccatttcccc ctcgcgcgtc tgggcttcga cgatgccatc cgagtaaagc
      361 agaatggcgg ccgggaaatc cgtaatcgtg cggttttcat acccgaaccg cttcgtgatg
      421 cccagggggc gtgagtcagc acgcagatac cgacaggtgc catcagcacc cgcaagaatg
      481 ggagaacatt ggccggcatt ggcgaacgtc accactcccg tgtcgatatc aagaatagcc
      541 agaaacattg tcatcatctt cttgcgctgg agaatgtcga cgaacgcgtg attgatgagt
      601 tcgagaaccc ttttcgggtc gtcggcgaac agcgggcaca ggatggaaaa tccggccttc
      661 gccatggccg tgaccagggc ggccgatacc ccatgtcccg agacgtctcc caatgcgaat
      721 gcgacttgtc gtgaagagat cgagatcgca tcgtagtagt cgccaccaac cgcctgcgtc
      781 atcagattcg ttccccgcca ttcaaaagag ccgacccgca gagactcttg cggcaacagc
      841 cgcttctgca ccgttgacgc gacttccatg tcgctcagat ggagaatcgc gcgattgaac
      901 gcatggcaca attgtccgag ttcatcgttg ctgtatacag gaatagatga ctcatagcgc
      961 ttggtgcgga taatttccag accgcgctcg agttctcgaa cgggcaccag caggagtcga
     1021 acaatcatca tggccagcaa agagccgagt acagccgcca cggcgagctg cagaaaaacc
     1081 tgccgacgaa accgcagaac acgatgttcg atggcactga ccggggtcgt ggcatagagc
     1141 gcgtatttca tcagcttcgc cccaatcatc ccgacgactt caatcggctc tccttcaagt
     1201 tccattcgct gaaacaggac gttacgacgg ccccgtcgca ggcgctccgt gagcttctga
     1261 tacatgggtg cgtgcgcgta atccggatag tagggcgagt agggatcatg ggtgctgata
     1321 aaacgaaacg tggttgccgt tcgcgcgcgg ttcaggtgtg cagaaagagc ctcgagatac
     1381 ggtccttcaa tcgtgcccac ttctgcccca aggagaatgc cataccatgc gtgtccttcg
     1441 ggcccccgaa tgatgtcgaa gagatagtag ttcccatcca acagggttcc agcgaagaaa
     1501 aatcctgtcg tcttctgtcg cagaaccctt accatatcga aatttgaatc gccgatcatc
     1561 acgtctaggg catttaccgc gccgtcgtcc ggtttgtctg cctcaaggcc ggcatcacta
     1621 tcatgctgct tcaatgccga taaggtcatg cgggcagttg cctgcttgat caggtcttct
     1681 ttctgatttg gatagcagtc gggttccggt tcaccacgaa acctgcggag atcggcttct
     1741 ccaggaatca acccggcggt gatccacccc agaaagacac gctccttctc tgattgcggg
     1801 attcgcgcca gacgacggcg caaggctcga ctggtattga actcgaaggc gagaaacacc
     1861 gaagctgaag agaccatgta atggttatcg aagccacctg agccgaacca cgcgcgaacc
     1921 tgcttcttca gcgcctcggg ctcatggcga aaggcctcga gacggcgaat tcgagctcgg
     1981 tatcgcaggc actcgcatgc cacgaactgc gcataaccct gatccagctg gcgcagacga
     2041 tcaaggcttt cccttcggca gtcctgaaca agctcattcc gtttttcctc gacgagaaag
     2101 tgccccagca gatacacgcc ggcaagcgga aaccccagga tcagagtaaa gagtccggca
     2161 agtttccagg acaatcgaac tggtcgatgc ccatcgagca ccagcatctc gtagcttcga
     2221 gaaagaaaca gcacactgag cagcccataa ccggcgagaa agactcgcca gggaacaccg
     2281 gcaacgggtt gaggttgtgc gatccagagt gcgtggtcac gggttgccca caggcaggtg
     2341 gtgacgtatg cgccatgaag cgcgcgttcg tgtccgagtt cgatctgccg aaacagacat
     2401 gcgctgaatt catccatggc gccgggcatg ttcccctgga cggaagtggt gtgggtgaaa
     2461 aagcctgaaa aagttcccgg ttcatcgtgt gccgacagcc aggagattct ctgttcgcgt
     2521 gtcatggcat tttcgcgagc aatcaggaag ccgccgtagt ccatgggcga tgaaggttcc
     2581 caccagtatc cccagtgcgt cccctgtccg tgtcccttga gttgatgcag gagaaccgga
     2641 atatccgctc ggttccagag acgtggcaga gcgtatcctg cgtgctccca gctcgtacgt
     2701 acagtttcgg gaacggcggt gagatacaga cttgcacctg tctgcctcag gctgtgaatc
     2761 acggtttgtc ggctttcgct gggtatcgtt ccactcgcca gccatattgt gcccgacgca
     2821 ttgaaaagat accactcgag aaaaccggcc ggaaaagagt tcgcccagtc ctgcacgact
     2881 gccgtctgcc gttggtgctg gtcatgggac gaaagtccga gtagacgcaa gcgagtgcga
     2941 aggttctgag cggctgcatg caggaacagg tcggagtcaa gaagcgtgct ccgttcttcc
     3001 aatacttgtt tctggttctc atacatggct tccagaagtc gttcacgtgt cagcaactcg
     3061 tactgcgcca gcccatacca ggtgaaaaag gcgggaatgg cccagatgat gactattgct
     3121 atcagccagc gggcaaaggg attcatggct ggtggcctcc tcgcgtcaag aggagcagag
     3181 tctggctttc tgcctgatcg ccggtcatgg tggcgaatgg agtgtgagcg aagagcctgc
     3241 gtgcgcattc cggctgatcg aactgatgct cagtcagact gccgagcagt tggagaacga
     3301 cagactctcc cgatgccacc gaggtctgaa gtagggcggg cgacaacacg gcgagcagct
     3361 cgaggtttgc gggggtttct tccgtaccag cctgcaggca gatgcggcaa agccccgtgt
     3421 tcgacgacga cgaatagaga ccgaaccccc cggtgtgaaa gagggtggcg gaaccggtat
     3481 ccatctcgat acgcccggca caccaggaaa atggctgttg tggcgcgaga tgacgattca
     3541 cgtaagcctg cagctgctgc acgatcaggg caggggaaat cactgaaccg g
//
LOCUS       DAORHD010000388         3590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28827_length_3590_cov_2.917115, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000388 DAORHD010000000
VERSION     DAORHD010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28827_length_3590_cov_2.917115"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PKO06_09275"
     CDS             complement(<1..1482)
                     /locus_tag="PKO06_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV69876.1"
                     /translation="MFSIPPQRLQSSLIFYITCIIVFTNGFFAIITLSRENIAFYNEG
                     INRALFLGKVLSRPASQALRTGTFAEFDECLAPGRLQGERNPVTLFDRNWWPVRGDPA
                     RLPPEGYPPSLATTEIANRSGVTGTSSRELFFPLYDGDTFLGAIEIGLPEFHSPKVVD
                     AAWQVLFVAGINILLGVALAIFIAQVIVRPLNQLLDGLEAVGRGDYSQRVQVFGEGEL
                     AQVGRMFNTMSASLQEKVSESLDRTRMLDEKVQELWEIYELTKAMGFNLNLHEILERF
                     LEKALALSSSSYGQIMLHVAETGRLEWSVGTPVFPRLNRQTYEEALDQCVRERAPFEK
                     KTDNYTLLFFPLLSGNAIHGVLFLGKVGCQSYSEGVQRFLDTISPLGGSLIENARLYQ
                     HVVEMKDYVRHVLESVDAGVATIDHEGRLITINSAFVRHLGLSDLRPQHQELEVALQD
                     LPDRDFCDKLCRLVRGRDSGGQVQRSMSGRFYVSQRREMPLHVS"
     gene            complement(1484..2500)
                     /locus_tag="PKO06_09280"
     CDS             complement(1484..2500)
                     /locus_tag="PKO06_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV69877.1"
                     /translation="MTETWKEQFSQRIMNLKTGFGSLLELVEHWLAALSREDERQKLI
                     DELVAIVSAKESGLREEIRTECLRKLDALAGKGTAVSVTSPAYRLNLQKKFQHGIERL
                     NREVDDLMIHLGKLLDTAAEKESPAGYFAAQEDERKRISREIHDGPAQALASLTMRID
                     FCLENMSKPEVLQPELSDLKESIIRCLRDIRRFIFDLRPMAIDDLGLIPTLEQYTAGF
                     RTRSGLKIHVHVDGERFSLGTDRELAVFRVIQEAVNNAHHHAAAKNVHIFLTFEGERK
                     TLVGVVKDDGTGFQVDVIRRQYSTLKKMGLQSMEERIHLAGGTFELVSNAGEGTIVSF
                     QIPL"
     gene            complement(2575..>3590)
                     /locus_tag="PKO06_09285"
     CDS             complement(2575..>3590)
                     /locus_tag="PKO06_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69878.1"
                     /translation="ATAAPAPAVPARRGHVLYEAPPGAPENPASVPAAPAASKQRVDL
                     PAGKVAPSASETISLASEKPGDVATPSTEPKLSELVKPEDADLPPEALAMLTPPPADG
                     RHLVRNATPGNEALALATGSALLFDAERMPERKSAIKVESRVASAAKSDDDALAHATA
                     VEETPEPSTGAIPEASGAKDLPRILEQGRSKYYQGAWEEALPLFLEYLKHHPSGEVYD
                     LVGNVFVKLKMNDDAYAASEHAYQLGYKQPATLIRLGSLAQGLKRYDAGATYLKEALD
                     KVPHRMDVKLTYAACLRGLGEYTKAETIYRQLLSGSPLSPALKSRVEKELQTLQQMNP
                     GKR"
BASE COUNT          779 a         1083 c          961 g          767 t
ORIGIN      
        1 actgacgtgc aggggcatct cacgtctctg actgacgtag aaccgccccg acatcgagcg
       61 ctgcacctga ccgccggaat cacgcccccg caccaaccgg cacagcttgt cgcaaaagtc
      121 gcgatcgggc agatcctgaa gggcgacttc cagttcctgg tgctggggtc gaagatcgga
      181 cagcccaaga tgccgcacaa acgctgaatt gatcgtgatc agccgtccct cgtggtcaat
      241 cgtggcaaca ccggcatcga cactttcgag cacgtgccga acatagtctt tcatttcgac
      301 cacgtgttgg tacaatctgg cattttcgat aagcgagccg ccaagcggag atatggtatc
      361 cagaaatctc tgcactccct cggaataact ctgacatccc actttcccga gaaagagaac
      421 tccgtgaatg gcgtttcccg aaagcagagg aaaaaacaac agcgtgtaat tgtccgtttt
      481 cttttcgaat ggcgcacgct ctcgaacgca ttgatcgagt gcctcttcat acgtctggcg
      541 attcaaccgc gggaaaaccg gcgtgccaac cgaccattcg agccgccccg tttcagccac
      601 atgcagcatg atctggccat aggatgatga agacagtgcc aacgcttttt cgagaaatcg
      661 ttcgagaatc tcgtgcaggt tcagattgaa ccccatggct ttggtcagct cgtagatctc
      721 ccacagttcc tgcactttct catccagcat acgcgtacgg tcgagacttt cgctgacttt
      781 ttcctgcagc gaggctgaca tggtattgaa catgcggccg acctgcgcca gttctccttc
      841 gccgaacacc tgaacacgtt gcgaatagtc gccgcgaccc accgcctcga gaccatcgag
      901 cagctggttc aacgggcgta cgatgacctg ggcaataaag atggcaagcg ccactcccaa
      961 caggatgttg atacctgcca cgaacaggac ctgccaggcc gcatcgacaa ccttcggcga
     1021 atgaaattcc ggcagcccga tctcgatggc ccccaaaaac gtgtcaccgt catagagcgg
     1081 aaaaaacagc tcgcgcgacg aggttcccgt caccccggaa cgattggcaa tctccgttgt
     1141 cgccaggctg gggggatagc cttcgggtgg gagccgcgcc ggatcgcccc gcaccggcca
     1201 ccagttccgg tcaaacagag tcaccggatt acgctcaccc tgcaggcgtc ccggggccag
     1261 acactcgtcg aattcggcaa atgtaccagt ccttaaagct tgagacgccg gacgagaaag
     1321 caccttgcca agaaataagg cacgatttat tccttcgttg tagaacgcga tgttttcgcg
     1381 agagagagtg atgatcgcaa aaaagccgtt ggtgaagacg ataatgcagg tgatgtaaaa
     1441 gatcaggctg ctctgcagcc gttgcggggg aatgctgaac acgtcagagc gggatctgga
     1501 acgaaacgat ggttccctca cccgcgttcg aaacgagttc gaacgttcca ccagccaggt
     1561 gaatccgttc ctccatgctt tgcagcccca tctttttcaa cgtcgagtac tgtcgccgaa
     1621 tcacgtcgac ctgaaaaccc gtgccgtcat ccttcaccac tccgaccaga gtctttcgct
     1681 caccctcgaa ggtcagaaag atatgcacgt tcttggccgc ggcatgatga tgtgcgttgt
     1741 tcaccgcttc ctgaatgacc cgaaacaccg ccaattcgcg gtcggttccc agggagaagc
     1801 gctctccatc gacgtgtacg tggatcttca accccgagcg cgtacgaaaa cccgccgtgt
     1861 actgttccag ggtgggaatc aaacccagat catcgatcgc catcgggcgc aggtcaaaaa
     1921 tgaaccttcg aatgtctctc agacagcgga taatcgactc tttgagatcg gacaattccg
     1981 gttgcagcac ttctggtttc gacatgttct cgagacagaa gtcgatgcgc atggtcagac
     2041 tggccaatgc ctgggccggt ccgtcatgaa tctcgcgcga aatgcgcttg cgttcatctt
     2101 cctgcgcggc gaaatacccg gcgggagatt ccttctccgc cgccgtatcc agcagtttgc
     2161 ccagatgaat catcagatca tcaacctcgc ggttcaaccg ttcaatgccg tgctggaact
     2221 ttttctgcag gttgagtcga tacgcaggcg atgtcacgga aaccgcggtt cccttgccag
     2281 ccagcgcatc cagctttctc aggcactcgg tgcgaatctc ttcgcgcagt cccgattcct
     2341 tcgccgacac gatcgccacc agttcatcga tcagcttctg gcgttcatct tcgcgactga
     2401 gcgcggcgag ccaatgttcg accagttcca gcaagctacc gaaaccggtc ttcagattca
     2461 taatgcgttg cgagaactgc tctttccagg tttccgtcat gttggcctca caaggtggtg
     2521 tatccgaaac gatggtccag agctagcagc acatcaggtg ttcgccgccc acgctcagcg
     2581 tttcccgggg ttcatctgct gcagggtttg cagctctttt tccacgcgac ttttcagtgc
     2641 tggcgacaag ggcgaaccgg aaagcagttg gcgatagatc gtttctgctt tcgtatactc
     2701 accgagtcca cgcaggcaag ccgcataggt cagcttcaca tccatgcgat gcggcacctt
     2761 gtcgagtgct tccttcagat aggtggcacc agcatcatat cttttcaatc cctgagctag
     2821 gcttcccagg cgaatcagcg ttgcaggctg cttgtaccca agctgatacg catgttcact
     2881 ggccgcatag gcatcatcgt tcatcttcaa tttgacgaag acattcccca ccagatcata
     2941 cacctcgccg ctcgggtgat gcttcagata ctccaggaac aatggcagag cctcttccca
     3001 ggctccctga tagtatttac tgcggccctg ttcgagaatt cgcggcagat ccttcgcgcc
     3061 cgacgcctcg gggatcgccc cggtggaagg ctcaggagtt tcttcaacgg cggtggcatg
     3121 cgccagagca tcgtcatcag atttggcggc cgaagccact ctggactcga ctttgatcgc
     3181 actcttgcgc tctggcatac gttcggcatc aaacagcagg gctgatccgg tggccagtgc
     3241 caacgcttcg tttcccggcg tcgcattgcg taccagatgc cggccatctg ccggtggcgg
     3301 ggtgagcatc gccagggctt caggaggaag atccgcgtct tcgggcttga cgagctcgga
     3361 aagcttcggc tccgtcgacg gtgtcgcaac gtcaccaggc ttctccgagg ccagtgaaat
     3421 cgtctcagaa gcggaaggag ccactttgcc cgcagggaga tcgacccgct gcttcgacgc
     3481 ggcaggtgcg gcaggtaccg aagcaggatt ctcgggggca ccgggaggag cctcatacag
     3541 gacatgtccg cggcgcgcgg gaacagccgg tgctggtgct gccgtcgccc
//
LOCUS       DAORHD010000389         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28862_length_3586_cov_4.303597, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000389 DAORHD010000000
VERSION     DAORHD010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28862_length_3586_cov_4.303597"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2425)
                     /gene="smc"
                     /locus_tag="PKO06_09290"
     CDS             complement(<1..2425)
                     /gene="smc"
                     /locus_tag="PKO06_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNV69879.1"
                     /translation="MYLKSLELLGFKSFGRKTVFDFQPGITAIIGPNGSGKSNVCDAI
                     RWVLGEQSAKALRGNKMAEVIFAGSPELRPAAFAQVKLLIDNEDRAMPVDYSEVSLGR
                     QLFRSGESNYFLNSNRSLLSELKEMLMDTGIGKDGYSIIGQGDISDIISQRLTSRRSL
                     IEEAAGITKFKHRKSNTLQKLDHTRANITRLTDIISEIEGTLAPLAEAAEKTRKAQAL
                     SAEIRTLEVDLILFDLAKYYGEFDNLDSMRRGLMSKIAEIQAFLAEIGGRKAEVLNRI
                     AGLEERVKTEQEKMREQQAALEEIRTRIGSRKEDVSRFQARRQAIQEEMAGIEKNLLA
                     TDEERQDAQNRLHDEEAQEIAVQSRMADIEANLKKVEGELEAHLKEVSQDRDSSFKVA
                     QLMTDKRNLINTSNQQVTMLQRQLDKSAGDAETLHNQLSKLQSEKQRVEHEIATMEKE
                     IATAEAALLEERQKLARCERDLSRNEEELAATDDQIKVHQARRNLLEQLRSRSESGTP
                     RGVREALLMKERELPGIFGVVGELLTVPRGYEMAFETALGGSIQDVVTRDADTAQKAI
                     AALKERKAGRATFLPIDMIQPPPRVENPKARGCLGVALDLIEYDAKFYSIMNHLLGRI
                     LIFDTLDNAVAYSRQNRNFNRIVTLEGDVVRSSGAMTGGSEGQKGMGMLSQKREMEDL
                     EEKLKTFQGREKKLSSMIGNLKQERLQLTQKVRQQEDFIARRKQSLDFFRHSLVKVSG
                     DLDAKAGEVNHVDSDRGEMQNECERQRTIATEAQKELNTLEEQHRGLTARLNSFSGRE
                     EGIQTRLNSL"
     gene            2776..3150
                     /gene="secG"
                     /locus_tag="PKO06_09295"
     CDS             2776..3150
                     /gene="secG"
                     /locus_tag="PKO06_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNV69880.1"
                     /translation="MLLAFLVKAVHFIVCFGLIVIVLLQANKGEGLAGAFGGGASSTI
                     FGERGDGGIMAKFTTGAAIIFMVTSLILSVWLPHWEKASASQVNVIQTSGSTKLPEAD
                     APIAPIAPVTPAPIAAPAPTGK"
     gene            3275..>3586
                     /gene="eno"
                     /locus_tag="PKO06_09300"
     CDS             3275..>3586
                     /gene="eno"
                     /locus_tag="PKO06_09300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331813.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNV69881.1"
                     /translation="MSCDCSDSPIIEYIHAREILDSRGNPTVEVEVVLESGVGGLAAV
                     PSGASTGEHEAVELRDGDKSRYLGKGVQKAVANVNDILAEKLEGWDATDQRGLDNLML
                     EI"
BASE COUNT          643 a         1002 c         1069 g          872 t
ORIGIN      
        1 gcaggctgtt gagacgggtt tggatgccct cttcgcgccc cgaaaagctg ttgaggcgtg
       61 ccgtcagacc gcgatgctgt tcttcgagcg tgttcagttc cttctgggcc tcggtggcga
      121 tggtgcgctg ccgttcacat tcattctgca tctcgccgcg gtcagagtcg acgtgattca
      181 cttcgccggc cttggcgtcg agatcgccgc tcactttgac cagactgtgt cggaaaaagt
      241 cgagcgactg tttgcgtcgg gcgataaagt cttcctgctg gcgaaccttc tgggtcagct
      301 gcaggcgctc ttgcttgagg ttgccgatca tggaactgag cttcttctcg cggccctgga
      361 aggtcttgag tttctcctcg aggtcttcca tttcgcgttt ctgcgaaagc atgcccatgc
      421 ccttctgccc ttctgatccg ccggtcatgg cccccgacga acgcaccaca tcaccctcga
      481 gggtgacgat gcggttgaag ttccggttct gacgcgagta tgcgaccgca ttatcgagcg
      541 tatcgaagat cagaattcgc ccgagcagat gattcatgat gctgtaaaac ttggcgtcat
      601 actcgatcag atcaagcgca acgcccaggc agccacgcgc cttggggttc tcgacgcggg
      661 gcggcggctg aatcatgtcg atgggcagga aggtcgctcg tccggctttg cgctctttca
      721 aagcggcgat tgccttctgc gccgtgtcgg catcgcgggt gacgacatcc tgaatactcc
      781 cgccaagagc ggtctcaaag gccatttcat agccacgagg aacggtcagc aactcgccga
      841 cgacgccgaa gattccgggc agttcacgct ctttcatgag cagggcttcg cgaaccccgc
      901 gtggtgtgcc tgattccgag cggctgcgca gttgttcgag caggttgcgt cgagcctgat
      961 gcactttgat ctgatcgtcg gtggcggcca gctcttcttc gttgcgggac agatcccttt
     1021 cgcatcgggc cagtttctgg cgttcttcca gcagggcggc ttccgcggtc gcgatctctt
     1081 tttccatggt cgcgatctcg tgctcgaccc gttgtttttc cgactgcagc ttgctcagct
     1141 ggttatgcag cgtttcggcg tcaccggccg atttgtcgag ctgacgctgc agcatggtca
     1201 cctgctgatt cgaagtgttg atgagattgc gcttgtcggt catcagttgg gcgaccttga
     1261 agctcgagtc gcgatcctgt gacacctctt tcagatgtgc ctcaagttcc ccttcgacct
     1321 ttttcaggtt ggcttcgatg tctgccatgc ggctctggac cgcgatttcc tgcgcttctt
     1381 cgtcgtgcag gcggttctgg gcgtcctgtc gttcttcgtc agtggcgagc aggttcttct
     1441 cgatgccggc catttcttcc tgaatcgcct ggcggcgagc ctggaagcga ctgacatcct
     1501 ctttgcgcga gccgatgcgg gtgcgaatct cttcgagggc tgcctgctgt tcgcgcatct
     1561 tttcctgttc agtcttgacc cgttcttcga ggccggcgat gcgattgagc acctcagctt
     1621 ttcggccgcc aatctcggcc agaaaggctt gaatctcggc gatctttgac atcaggccgc
     1681 gtcgcatcga gtcgaggttg tcgaactcac cgtagtattt cgccagatca aacagaatca
     1741 gatcgacttc gagcgtgcga atctcggctg aaagagcttg cgccttccgg gttttttcag
     1801 cggcttcggc caggggagcg agtgtgccct cgatctcgga aatgatgtca gtcaggcgcg
     1861 taatgttcgc ccgggtgtga tcaagcttct gcagggtatt gcttttgcgg tgcttgaatt
     1921 tggtgatgcc ggcggcctct tcgatgagcg accggcgact ggtgaggcgt tgcgagataa
     1981 tatcgctgat gtctccctga ccgatgatcg agtagccgtc tttgccgatg cctgtatcca
     2041 tcagcatttc cttgagttcg gaaagcagcg agcgattgct gttgaggaaa taattgcttt
     2101 ctcccgagcg aaacagctgc cggccaagcg acacttcgga gtaatcgacc ggcatggccc
     2161 ggtcttcgtt gtcgatcagc agcttcactt gcgcgaaagc cgccggacgc agttcgggac
     2221 tgccggcgaa gatgacttcg gccatcttgt tgccgcgcag ggctttcgcg ctctgttcgc
     2281 cgagcaccca gcgaatggcg tcacagacgt tgcttttgcc cgaaccgttc gggccgatga
     2341 tggcggtgat accgggctga aaatcgaaaa cggtcttgcg accgaaggat ttgaacccca
     2401 gcagttcaag ggatttcaaa tacatgggcg aacgggctcc tcgagcggga aaatatcgac
     2461 tgccgccgcg gacatagcac gtggcggagc aggaccatat aagcacaatt ctgaagaaaa
     2521 gaacagaggg agagggtggt ttcaccgaat ggcaacaacg tcgtacagct gaattggact
     2581 ctgcgtgttt gtgtcgaatc ttgagaggaa aggtctctcg gacgcgtggt tcccgcatgc
     2641 ggagcggttg cggcgcacgg ggttggataa gcgggtgctg tgccgggtac ataaactgat
     2701 tgacaatttt ctgcggcgtt gctagactcc acctcacctt tacagggcat atctttggcg
     2761 aaggagcatt ttcggatgct gttggccttt ctggtgaaag cggttcattt cattgtgtgt
     2821 ttcggtctga ttgtcatcgt tctgctgcag gcgaacaagg gcgaaggcct ggcgggcgca
     2881 ttcggcgggg gcgcgtcgag cacgatcttc ggcgaacgcg gcgatggcgg tatcatggcc
     2941 aagttcacca ccggagcggc aatcatcttc atggtcactt cgctgatcct ttctgtgtgg
     3001 cttcctcact gggaaaaagc gtcagcaagc caggtcaatg tcattcagac ctcgggttcc
     3061 accaagcttc ccgaagctga tgcgccgatt gcgcccattg ccccggtcac cccggctccg
     3121 atcgcggcgc ctgcgccgac cggcaagtaa ccatgtcacc ttcgaccccg gaactccacg
     3181 tttccggggt cgttgctttt ctggcaggtt gattctgtga tacattttga cggcccgcag
     3241 gggccgcaca taccaacatc aggaggccaa gtccatgtct tgtgattgct ccgattcccc
     3301 tatcattgag tatatccacg ctcgcgagat tcttgacagc cgcggtaatc ctaccgtcga
     3361 agtcgaagtg gttctcgaaa gcggtgtcgg tggtctggca gccgttccgt ctggagcatc
     3421 gaccggtgaa cacgaagccg tcgaactgcg cgatggcgac aagagccgat atctgggcaa
     3481 gggcgttcag aaagccgttg ccaatgtgaa cgatattctg gccgagaagc tcgagggctg
     3541 ggatgcgacc gaccagcgtg gtctcgacaa cctcatgctc gagatc
//
LOCUS       DAORHD010000390         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_28954_length_3578_cov_3.160375, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000390 DAORHD010000000
VERSION     DAORHD010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28954_length_3578_cov_3.160375"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PKO06_09305"
     CDS             complement(<1..349)
                     /locus_tag="PKO06_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69882.1"
                     /translation="MSRKQCLAFSLLICAWLSLGVAGAQANPFFFKGTDSGCQFRAAP
                     DGSWQAVDAPKLICGAQMALRTEKGSWGTIVYSFGSMTLHPESEVLFQPNGLILKQGK
                     YRAFIHASPRGFQF"
     gene            complement(456..2135)
                     /locus_tag="PKO06_09310"
     CDS             complement(456..2135)
                     /locus_tag="PKO06_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HNV69883.1"
                     /translation="MVRTRSLLVLCLLAAVSLFTATAWADVNFVGVVNPLEKLIKDAE
                     AKQNWKAVEAYTTRLITYLRANGGHKYDAQIVSWYEKRSQAEAKQGKYNEAANTVRES
                     HAWKADSGNVDKVMKFERNDMDQRDYYNKTLEFYNKRLTLKQEILTLAQEKEKLVKDM
                     LQKKDVKKADLEKMQADIKAINEKIAAKREEVKKVHEQYQKDVDGYRKDGLVFNSNQS
                     TLLQEKARKIQNVNDKIQTTQNNVQKGLLEIVEKYKVSWEGLEERLVKMQDVQNKIMD
                     LHKKILALLDKKPFGEAERKELEALKADLDKLMLEHEKLMTEIENAFMDTKTFNKLTI
                     DQQKKYMDIFDAIRKTQTVIAETAKLIEKKMKEVLFVYGDLTGDGKVDKADLEKMWRE
                     YLVTPIFKSWPPIWRPVPYNKAADVDGDGRITWADYQLMKEVVGGARKTFPVDPNNLP
                     GDMDGDGALDSDDINRLARVMLNPGDYVRTAFIKIADLNGDGKVNFEDLIALVKKLGE
                     KKDEAAAPADGSTAAPAPTDAATGTGEATGQTDATDPSQTGTAADNINSAY"
     gene            complement(2281..2949)
                     /locus_tag="PKO06_09315"
     CDS             complement(2281..2949)
                     /locus_tag="PKO06_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV69884.1"
                     /translation="MVSIPASPGAQNTRFSDRGHGSVLRGFTLIEILVVVVVSMMVLF
                     AMYAFFRSGVRSTVKGQDTLDSIRAASVLFSQLRKDLLACKNIDTGTASLTIGVGVGL
                     PPTLPFADSITFSHRNATMTYKFLVSAGRGFVQREEYRPNAAPLVRDFAVPRMKKFEA
                     LQIWKKHKPVATSVHYMVSNQVLIRIEIDSQDPRFPTKSVHLSSFFTSNQKTSSDWWN
                     YFYD"
     gene            complement(2955..3482)
                     /locus_tag="PKO06_09320"
     CDS             complement(2955..3482)
                     /locus_tag="PKO06_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV69885.1"
                     /translation="MKSARCRKAMTLIEVLVTIAVLSLALGPLVQLLSSSNRMSNASV
                     YEVMAVHYAAEINEQLQWLAQNNTIAMVKAATGRDLEDLLTDPLLQAQMENMTPGLGP
                     YLAKFPGTEISLLLSPLHPNFTGRRLYVKRLDSTSATTLNTGVYWDVTVSLSWKLSQS
                     DARDHMASFSTILRE"
BASE COUNT          760 a         1019 c          929 g          870 t
ORIGIN      
        1 taaactggaa cccgcgcggt gacgcatgaa tgaaggctcg atacttgccc tgtttcaaga
       61 tcagcccatt cggttggaac aggacttcag actcggggtg caaggtcatc gagccgaagg
      121 aatacacaat ggttccccag gatccctttt ccgtgcgcag agccatctgc gctccgcaga
      181 tgagcttcgg tgcgtcaacg gcctgccatg agccatcagg agcggcacga aactggcacc
      241 cgctatccgt tcccttgaaa aagaacggat tcgcctgagc tccggccacg ccaagactta
      301 gccacgcaca gatcagcagc gagaacgcga ggcactgttt acggctcatg tattttctcc
      361 tcatatagac atcttctcat cgactgacga gactggaacg gcgccggtcg cctgaccggc
      421 gccgtccact cttcacttac agacgaacga ggtctttagt aggccgagtt gatgttgtcc
      481 gcagccgtgc cggtctggct cggatcggtc gcatcggtct ggcctgtcgc ttcaccggta
      541 ccggtcgcgg catcggtcgg ggcgggcgca gccgttgaac cgtcagccgg agccgccgct
      601 tcgtccttct tctcgcccag cttcttcacg agcgcgatga ggtcttcgaa gttcaccttg
      661 ccatcgccgt tcagatcagc gatcttgatg aacgccgtgc gcacatagtc accggggttg
      721 agcatgacgc gagccaggcg gttgatgtca tcgctgtcga gcgcgccgtc gccatccatg
      781 tcgccgggca ggttgttcgg atcaaccggg aaggtcttgc gagcgccgcc cacaacttcc
      841 ttcatcagct gatagtcagc ccaagtgatg cggccgtcgc catcgacatc ggcagccttg
      901 ttgtaaggaa ccgggcgcca gatcggaggc catgacttga agatcggggt gaccaggtat
      961 tcacgccaca tcttttcgag atcggctttg tcgaccttgc cgtcaccggt caggtcaccg
     1021 tacacgaaca gaacttcttt catcttcttc tcgatcagct tggcagtctc ggcaatgacg
     1081 gtctgcgtct tgcgaattgc gtcgaagata tccatgtatt tcttctgctg atcgatcgtc
     1141 agcttgttga aggtcttggt atccataaag gcgttttcaa tctcggtcat gagcttttca
     1201 tgttcgagca tcagcttgtc cagatcagcc ttgagggctt ccagttcctt gcgttcagct
     1261 tcgccgaagg gcttcttgtc gagcagggcc agaatcttct tgtgcagatc catgatcttg
     1321 ttctggacat cctgcatctt gacgaggcgc tcttcgaggc cttcccagga gaccttgtac
     1381 ttttccacga tctcgagcag gcccttctgg acgttgttct gcgtggtctg aatcttgtcg
     1441 ttcacgttct ggatcttgcg agccttttcc tgcagcagcg tgctctggtt ggaattgaag
     1501 accagaccgt ccttgcggta gccatcgaca tccttctgat actgttcgtg aaccttcttc
     1561 acctcttcac gcttggcggc gatcttctcg ttgatcgcct tgatatccgc ctgcatcttt
     1621 tcgaggtcag ccttcttcac atccttcttc tgcagcatat ccttgaccag cttctctttt
     1681 tcctgagcca gggtcagaat ttcctgcttg agggtcaggc gcttgttata gaactcaaga
     1741 gtcttgttgt agtaatcgcg ctggtccatg tcgtttcgtt cgaacttcat gaccttgtcg
     1801 acattgccgg aatcagcttt ccaggcgtgc gactcacgaa ccgtattcgc ggcttcgttg
     1861 tatttgccct gcttggcttc agcctgagag cgcttttcat accaggaaac gatctgagcg
     1921 tcatacttgt ggccaccgtt cgcgcgcaga taggtaatca ggcgcgtcgt gtaggcttcg
     1981 accgctttcc agttctgctt cgcttccgca tccttgatca acttttcgag cggattcacg
     2041 acaccaacaa aattcacatc agcccaggca gtcgcggtga acaaactcac cgctgccagc
     2101 aaacaaagca ccaacagaga ccgggttctt accataatcc taactcctcc tcatgaaatt
     2161 attgtggaca tgagatgacg aaacgtggct gagccaatcc aggtcagcag ttaaaaaacg
     2221 gtctgctcta cctccccgag tacgtatata tctccatatg agaatagcac gaaaaaagtt
     2281 tcagtcataa aagtagttcc accagtcact cgacgttttc tgattcgagg tgaaaaagga
     2341 actgaggtga accgacttcg taggaaatcg tggatcctgg gagtcaatct caatgcggat
     2401 caatacctgg ttcgatacca tgtaatgcac actggtcgcg accggcttat gtttcttcca
     2461 gatttgcagg gcttcgaact ttttcatgcg tggaaccgcg aagtcgcgca ccagaggagc
     2521 cgcgttcggg cggtattcct cgcgttgcac gaaaccccgt ccggcactca ccagaaattt
     2581 atacgtcatc gtcgcattcc gatgagaaaa cgtgatgctg tccgcaaacg gcagggtcgg
     2641 cggcaacccg acccctactc cgatcgtcag agatgcggtc cccgtgtcga tgttcttaca
     2701 cgccaacagg tctttgcgta actgagaaaa cagaacactc gctgctctga tcgagtcgag
     2761 cgtatcctgt cccttgaccg tgctgcgaac ccctgagcgg aagaacgcat acatggcaaa
     2821 cagcaccatc atggaaacga cgaccaccac gagaatttcg atcagtgtga agcctctcaa
     2881 aacgctgccg tggccccgat cagagaatcg agtgttctgc gcgccgggag aggcagggat
     2941 cgataccatg attttcattc cctcagaatc gtgctgaatg acgccatgtg atcgcgggcg
     3001 tccgactggc tcagtttcca cgaaagactt accgtcacat cccaataaac acccgtattg
     3061 agtgtcgttg cacttgtact atcgagtcgc ttcacataca gtcgacgacc ggtgaagttg
     3121 gggtgcagcg gagacagcag cagactgatc tccgttcccg gaaacttcgc cagatatggt
     3181 cccagtccgg gggtcatgtt ttccatctgc gcctgaagga gcggatcggt cagcagatct
     3241 tcgagatcac gccccgtcgc cgccttcacc atagcgatgg tgttgttttg cgccaaccat
     3301 tgcaactgtt cgttgatctc ggccgcatag tgaaccgcca tgacttcgta gaccgaggca
     3361 ttgctcatgc ggttcgatga actcagcagt tgaaccaacg ggccaagtgc cagagacaac
     3421 acggcgatgg tgacgagcac ttcgatcagg gtcatggctt ttcggcagcg agcagatttc
     3481 atcaatcggc tcccgacacc acgatggaac tgtgcttgtc ttcgagaatg aaacgccgtg
     3541 aagccgcata cagcggggaa cttggattga aacgcggg
//
LOCUS       DAORHD010000391         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29049_length_3571_cov_4.206200, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000391 DAORHD010000000
VERSION     DAORHD010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29049_length_3571_cov_4.206200"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..3299
                     /locus_tag="PKO06_09325"
     CDS             144..3299
                     /locus_tag="PKO06_09325"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNV69886.1"
                     /translation="MEAHILFWLAPLGAVLALMFGYFFYQGVESAEAGSEKNQAISVM
                     VKNSVQMFLSTQVRLAILLFIGLAVLLLVLAFLIRAVSPWICFAFLTGGLYSLFTVKW
                     GASTAVTAGSRVAAALGKSLSQALTIALRGAALIGLAAAAFAIIDITIWWKLLDIVRS
                     KADSFKESPFIIANYSILTTILLACALGSSLMSLLFRLSGVLLARGAGLGLDLTAKLE
                     ASVPEGDPRNPSVVAEQAGYLAAHTAAPGLEGFEALTLAVLTASAIAASTFNLPNLEK
                     EMLGAMFLPLILAGLGLIFSLVSVAMVTVRDDAAAKDVWAGFIKGNYVGLILTAFVAA
                     FLSFYLLPVHFKVYGSFLIGMLAGVIMGWAMETAASTEFETVKDLSKQATSGGAMLLT
                     EGFSLALRTSGAPLFVLGAVAILSYLFAGGFDHPGLGFYGMALAAVGMLMNQAVMTAG
                     SSLASVTRIASFQVDAAQIGADAKKRADALDQVGRLLGGFGRGYSLGVSCLVVLAMIG
                     GYLEQLRSSFPRLEAVTGEAADKFIFLEKQRPVAAELASYRDLATAFDINLASPQVLI
                     GMFLGAGLILILGSSLLRSMATVAQRLVEVVKAQYKEIAGLVKGEGGAQADHNGCMLA
                     LMQEVHREALWPIALVVIMPAVASIVFGPGGMIGLLIGALIAAVGLGLLSSGLSGGWE
                     ASRLIIESGQHGGKGTDAHKATILADQLGQSLREACGPVLAGVIKPMAIVGMILASFT
                     VIWGGDFQNLLNFPSWEWKNRTEAYRLHKDGLLKLPDIKAERAAAALQDGVIDEEDPL
                     MTSDEGLLPEDEEGVMIEEYASGTAEMFPATPVPASSASPAPTTPVETGGKPEVPAGG
                     FDGFDDAGAPADAGKKDPKPAAGGFDGFDEPSDKPADKPADKPANKTADKPADKPAAG
                     GFDAFDEPADKPADKPADKPADKPADKPADKPADKPADKPADKPADKPADATEPPADK
                     PADKPVEKPADAKPADKPADAPADVKPADKPADTPTDTPATDKPVEPPADKPADKPAD
                     KPADKPADAKPVSTGGGFDDL"
     gene            3405..>3571
                     /locus_tag="PKO06_09330"
     CDS             3405..>3571
                     /locus_tag="PKO06_09330"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNV69887.1"
                     /translation="MSVLEVSRLRRLSGNLRFPGDKSITHRCLFLAALAEGESRFAYP
                     ATGDDCLRTVNA"
BASE COUNT          649 a          997 c         1097 g          828 t
ORIGIN      
        1 caaaactctt cctcgtgatc agtggtctct gcgttgacac tctttccttg tgatggatat
       61 aatggctcgc cttggaaaag gcatgtgagt gttttgctga gaagtgacta ttttcccttg
      121 aggtattggt gtaaggaggc tccgtggaag cgcacatttt gttttggtta gcccccctgg
      181 gagctgttct cgccctgatg ttcgggtatt ttttctatca gggtgttgag tcggctgagg
      241 ccggttctga aaagaatcag gcgatcagcg tcatggttaa gaacagtgtt cagatgtttc
      301 tttccacgca ggttcgcctg gccattctgc tgttcatcgg tctggcggtg ttgctgctcg
      361 tgctggcatt cctgatccgt gctgtcagtc cgtggatctg cttcgccttc ctgacgggcg
      421 gcctgtattc gctgttcacc gtgaaatggg gagccagtac cgcggtcact gccggcagtc
      481 gtgtcgccgc tgccctggga aaatcgctca gccaggctct gaccatcgcg ttgcgtgggg
      541 ccgctctcat cggtttggcc gctgccgcct tcgccatcat cgatatcacc atctggtgga
      601 aactgctgga catcgttcgt tccaaggcag attccttcaa ggaatctccg ttcatcattg
      661 ccaactattc gattctgacc acgattctcc tggcatgcgc cctgggcagt agtctcatgt
      721 ccttgctgtt ccgcctttcc ggagttcttc tggcccgagg tgcggggctg ggtctcgacc
      781 tgacggccaa actggaagcg tcggttccag agggcgatcc gcggaatcct tcggtcgtgg
      841 ctgaacaggc gggttatctt gccgcacaca ccgccgctcc tggtctcgag ggtttcgaag
      901 ctctgacact cgccgtgctc accgcttcgg ccatcgcggc atcgaccttc aatctgccca
      961 acctcgagaa agaaatgctg ggggcgatgt ttctgccatt gattctggct gggctgggcc
     1021 tgattttctc gctggtgagc gttgccatgg tgaccgtgcg cgatgatgcc gccgccaagg
     1081 atgtgtgggc cgggttcatc aaggggaact atgtcggcct gattttgacg gcgtttgtcg
     1141 cggccttcct gagcttctat ctgcttccgg tgcacttcaa ggtctatggt tcgttcctga
     1201 tcggcatgct ggctggggtg atcatgggct gggccatgga aacagccgct tcgacggaat
     1261 tcgagaccgt gaaagatctt tcgaaacagg ccacgtccgg tggagccatg cttcttaccg
     1321 agggcttctc gctggcactg cgcacttcag gcgctccgtt gtttgtcctc ggggcggtgg
     1381 cgattctgtc gtatctgttt gccggcgggt tcgaccatcc gggattggga ttctatggca
     1441 tggcgttggc cgcagtcggc atgctgatga atcaggcggt catgaccgcg ggctcctcac
     1501 tggcgagcgt gacgcgcatc gcctcttttc aggtcgatgc cgctcagatc ggtgctgatg
     1561 cgaaaaagcg ggccgatgct ctcgatcagg tcgggcgttt gctcggcggc ttcggtcgcg
     1621 gttactccct gggggtttcc tgtctggttg tcctggccat gattggaggc tatctcgagc
     1681 agctgcgctc ttcgttccca cgtcttgaag ctgtgactgg tgaagctgcc gacaagttca
     1741 tctttctgga aaaacagcgc ccggttgcgg cggagctcgc cagctatcgt gatctggcaa
     1801 cggcttttga tatcaatctg gcatctccgc aggtactcat tgggatgttc ctgggtgccg
     1861 gtctgatcct gattctcggt tcgagcctcc tgcgatcgat ggcgactgtt gctcagcgtc
     1921 tcgtcgaagt ggtgaaggct cagtacaagg aaattgcagg cctggtcaag ggtgagggcg
     1981 gagcccaggc tgatcacaac ggctgcatgc tggcgctgat gcaggaagtg catcgtgagg
     2041 ctctgtggcc gatcgcgttg gtagtcatca tgccagcagt ggcttcgatc gtgttcgggc
     2101 ctggcggcat gatcggtctg ttgatcggtg cgctgattgc cgcggttggg ttgggcttgc
     2161 tttcgtcggg cctcagtggc ggttgggaag cgtctcggct gattatcgaa tctggacagc
     2221 acggtggaaa agggaccgac gcccacaagg cgacgattct ggccgatcag ctgggacagt
     2281 cgctgcgcga ggcttgcggc ccggttctgg ccggcgtcat caagccgatg gccattgtcg
     2341 gcatgattct cgcgagtttc accgtgatct ggggtggtga cttccagaac ctgttgaact
     2401 ttccttcctg ggaatggaaa aatcgcacag aagcgtatcg tttgcacaaa gacggattgc
     2461 tcaagctgcc cgacatcaag gcagagcgag ctgctgccgc gcttcaggat ggagtcatcg
     2521 atgaagaaga tccgctcatg acctctgatg aaggtctgct tcctgaagat gaagaaggcg
     2581 tcatgatcga ggaatacgcc tctggaacgg ctgagatgtt cccggcaact ccggttcccg
     2641 cttcatcggc cagtccggct ccgaccactc cggttgaaac gggtggcaag cctgaggtgc
     2701 ccgcgggtgg gttcgacggg ttcgatgatg ccggagctcc ggccgatgcc ggcaagaaag
     2761 acccgaaacc tgctgccggc ggcttcgacg gctttgatga accgtctgac aagccagcag
     2821 ataaacccgc cgacaaaccc gcgaacaaaa ccgcggataa gccggctgac aaacccgctg
     2881 ctggcggctt tgacgcgttt gatgaaccgg cagataaacc ggcagataaa cccgcagata
     2941 aacccgcgga caaacccgcg gacaagcctg cggacaaacc cgcggacaag cctgcagaca
     3001 agcccgccga caagccggcc gataaacctg ccgacgcgac ggagccgccc gctgacaaac
     3061 ccgcggacaa gccggtcgaa aagcctgccg atgccaaacc tgctgataaa ccggccgatg
     3121 cgcctgcaga tgtgaagcca gcggataagc ccgccgacac gccgaccgac acgccggcga
     3181 cggacaagcc ggtagaaccg cctgcggata agcctgcaga taagcctgcg gacaaacctg
     3241 cggataagcc cgctgatgca aagccagtga gcacaggcgg cggttttgac gatctgtaag
     3301 gtgattgctg ttcgaagccg cccgggaaca aaccccgggc ggcttttttt cgagagacaa
     3361 acggttacga cgctccggtc gaaacgttgt cattttctca tggcatgagt gttctcgaag
     3421 tttcgcgtct tcgccgattg agcggcaacc tccgatttcc gggcgacaag tcgattacgc
     3481 atcgttgtct gttccttgcg gctctggctg agggcgagtc caggtttgct tacccggcaa
     3541 ccggcgatga ctgtctgcgc accgtcaatg c
//
LOCUS       DAORHD010000392         3569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29075_length_3569_cov_3.244735, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000392 DAORHD010000000
VERSION     DAORHD010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29075_length_3569_cov_3.244735"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PKO06_09335"
     CDS             <1..685
                     /locus_tag="PKO06_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69888.1"
                     /translation="LNTLLDNVPFEVIREGAPAGLLSFENLDKVPAVANYYKSLGVDA
                     AYVQKMSSMLFPGCDKITDKSWRCQGVIDDPRGISYLATLLVLDLVDNSSIKKPEQIP
                     WGTSFPEINQVTYSYCKNPAILDRPDWRISDFEWNLPNQIGVPPESQNMYKEPDFTSP
                     TLVRFTTRLLADKVNYTDNEDLAFCAMKKLIIQIQWNLDKSLYRKPDVLDKQTQRIHL
                     MTLKADLNR"
     gene            697..1650
                     /locus_tag="PKO06_09340"
     CDS             697..1650
                     /locus_tag="PKO06_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69889.1"
                     /translation="MNHPSALHSLVDKLSSYAGFRNCRLVRSGMAIVIALFFAFSMMI
                     MFVGLLTMNRQTAGHNRAALQQAQAYFAARAALQHFMMKAKLCPTELYDAVEFTQGKN
                     PLFDFSEFPSVYNGANAFAPYTTNSEIHIRVLPQKELDSKQKPKWFYRRLPGRNDVFI
                     RVGSPFLPEFRFIAPNVVNTDANKKFTEPNAAAYASYDPERYLKYYIQDCTNKPVNSK
                     LLQPRLVMNKAQAVATIRDWSIDRYFSDFEKVSGPERYPYSMFYEVKAVSLGHMKDLR
                     RYNEEAIEIQVIGSIVDFQGKTFTQEQKKVQKITRLPGATP"
     gene            1647..2378
                     /locus_tag="PKO06_09345"
     CDS             1647..2378
                     /locus_tag="PKO06_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV69890.1"
                     /translation="MITMRRRAFTLIEVMMALAVVGIFLAISYRLFIGSQRIAGKGTW
                     TSGVIDQMRNALNQLNLSLKSTSYPTTLLSDAIKDPVNGGMDVAKKFFVKLHKDGTVK
                     VANLTGETEIAQWVVCEPEKPPQRGIIIYHRLLLVPIAKTVVKEGEKVAELWIDAQAN
                     AFSTTGPKHAESGTLSLTALPDKNRRFRILEDVSEVRFTVPSALPALDPQPIKIELRC
                     QYPRDVNTFKENSLMITPNVGVDNL"
     gene            2403..3161
                     /locus_tag="PKO06_09350"
     CDS             2403..3161
                     /locus_tag="PKO06_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV69891.1"
                     /translation="MAKQWHQLEKLDHKSLKKLQQEREKAVVNQQKDAERKRIGVVVI
                     CVLLAIICGIALIFTIRSRAAKKAFLDAREKLAQLTVGDVSGDSQVRSVGDWERLSKG
                     FVFDKEHTFKTAKGSTIAVEMHYKNLVKIASGSEVVVSPPELHPTENKVVKERVILRS
                     GEVTVAVSLDGREILEIEAAGVVAMGASGLFKVLYNNTKGTGEVVVKNGLVEVFTRSN
                     PTKKIKVSGFYKVTFQQGKVSNPTQASVIQYNWQ"
     gene            3264..>3569
                     /locus_tag="PKO06_09355"
     CDS             3264..>3569
                     /locus_tag="PKO06_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69892.1"
                     /translation="MIRVIKAKVHNLKDLTVSIPRNSLTVITGVSGSGKSSLAFDTIH
                     AEGQRRYMESLSSYARQFLQQMEKPDVEAIEGLSPTIAIQQKSTSRNPRSTVGTITEV
                     "
BASE COUNT          869 a         1044 c          902 g          754 t
ORIGIN      
        1 cctcaacacc cttctcgaca atgttccttt tgaggttatt cgagaaggcg ctcccgctgg
       61 tctcttgtcg ttcgagaacc tggacaaagt accggccgtc gccaactatt acaagagtct
      121 gggagtcgat gccgcctacg tccagaagat gtccagcatg ctgtttcccg ggtgcgacaa
      181 gatcaccgac aaatcctggc gatgccaggg cgtcatcgat gatccccgcg gcatcagcta
      241 tctggcgaca ttgctggttc ttgatctggt cgacaactcc tctatcaaga aacccgaaca
      301 gattccctgg ggaacatcgt ttcccgaaat caatcaggtg acctattcct actgcaagaa
      361 tccggcgatt ctggacagac cagactggcg gatcagcgac ttcgaatgga accttcccaa
      421 ccagattggc gtcccccccg aaagccaaaa catgtataaa gaacctgatt tcaccagccc
      481 cacgctggta cgattcacca cgcggctcct tgcagacaag gtcaactaca ccgacaacga
      541 agacctggcg ttctgcgcca tgaagaaact gattatccag atccagtgga acctcgacaa
      601 gagcctgtat cgcaaacccg atgtcctgga caaacagacc cagagaatcc acctgatgac
      661 gctcaaagcg gatctcaatc gataaaggga atgaatatga accacccatc tgcacttcat
      721 tctctcgtcg acaagttgtc ttcttacgcc ggctttcgca attgcaggct agtacgcagc
      781 ggcatggcta tcgtcatcgc gctgtttttc gctttcagca tgatgatcat gttcgtgggc
      841 cttctgacca tgaatcgtca gaccgcgggc cacaatcggg cagcattgca gcaggcccag
      901 gcctactttg ccgctcgtgc cgccctgcag catttcatga tgaaggccaa gctgtgtccc
      961 accgagctct acgacgcagt cgaattcacc caggggaaaa acccgctttt cgacttcagt
     1021 gagtttccgt cggtctacaa cggggccaat gcctttgccc cctacacgac gaattccgag
     1081 atccatatcc gcgttctgcc tcagaaagaa ctcgattcca agcagaagcc gaagtggttc
     1141 tatcgccgtc ttccgggtcg caatgacgtc tttatcagag tcggcagccc ctttctgccc
     1201 gagtttcgct tcatcgctcc aaatgtcgtc aacaccgacg ccaacaagaa gttcaccgag
     1261 ccgaatgcag ccgcctatgc ctcctatgat cccgagcgct acctgaagta ttacattcag
     1321 gattgcacca acaaaccggt caacagcaaa ctgttgcagc cgcgcctggt catgaacaaa
     1381 gctcaggcgg tggccaccat tcgtgactgg tcgatcgacc ggtatttctc tgatttcgaa
     1441 aaggtcagtg gccccgaacg gtatccctac agtatgttct acgaagtgaa ggccgtcagc
     1501 ctcgggcata tgaaagacct tcgacgttac aatgaagaag caatcgagat tcaggtcatc
     1561 ggctcgatcg tggatttcca gggcaagacc ttcacgcaag aacagaaaaa agtgcagaaa
     1621 atcacccgtc tccccggagc caccccatga tcaccatgcg ccgacgcgcc tttaccttga
     1681 tcgaggtcat gatggctctc gccgtcgttg gcatctttct ggcgatctcc tatcgattgt
     1741 tcatcggcag ccagcggatc gccggtaaag gcacctggac cagcggcgtc attgatcaga
     1801 tgcgtaacgc actgaatcaa ttgaatctgt cgctcaagtc gacgtcgtat ccgaccacgc
     1861 ttctttccga tgccatcaag gatcccgtca acggcggcat ggacgtcgcg aagaagttct
     1921 tcgtgaaact gcacaaagac ggtacggtga aagtcgccaa ccttacgggc gagaccgaga
     1981 tcgcccaatg ggtcgtctgt gagccggaaa aaccaccgca acgcggaatc atcatctatc
     2041 accgcctgtt gctcgtgccc atcgcgaaga cggtggtgaa ggaaggtgag aaggtcgccg
     2101 aactctggat tgacgctcaa gccaacgcct tctcaacaac cggtcccaaa cacgcggaat
     2161 caggcaccct gtcgctgacg gcactgccag acaagaaccg ccgatttcgc atcctcgaag
     2221 atgtcagcga agttcggttc accgttccca gcgcgctccc ggctcttgat ccgcaaccga
     2281 tcaagatcga gctgcgctgt cagtatcctc gcgacgtcaa cactttcaaa gagaattctt
     2341 tgatgattac tcccaatgta ggagtcgaca atctctgatc cgggtcggtc aaacaatacg
     2401 tcatggcaaa acagtggcac caactagaaa agctcgatca taaatcgctc aagaagctgc
     2461 aacaggagcg agagaaggcg gtcgttaacc agcagaagga cgccgagcgg aagcgcatcg
     2521 gggtcgttgt catctgcgtt ctgctggcaa tcatctgcgg tatcgctctg atcttcacga
     2581 ttcgctccag ggccgccaag aaggcgtttc tggatgctcg tgaaaagctg gcgcagctga
     2641 ctgtcggcga tgtttccggc gactctcagg tacgctcggt cggtgattgg gaacgcctaa
     2701 gcaagggctt tgttttcgat aaggagcaca cgttcaaaac cgcgaagggt tcgaccatcg
     2761 cggtggagat gcattacaag aacctggtga aaattgcctc ggggtccgaa gtggtggtgt
     2821 cccctcccga actccacccc accgaaaaca aggtcgtgaa agaacgggtg attctccgct
     2881 cgggagaagt cacggtcgct gtttcgctcg acggtcgcga gattctcgag atcgaagccg
     2941 cgggagttgt cgccatgggc gcttcgggtc tgttcaaggt cttgtacaac aacaccaagg
     3001 ggaccggtga agttgtcgtc aaaaacggac tggtcgaggt ttttacccga agcaatccga
     3061 cgaaaaaaat caaagtctcc ggattctaca aagtcacgtt ccagcaggga aaagtcagca
     3121 acccgaccca ggccagcgtt attcagtata actggcagta atcgccgcgg gccttttcgc
     3181 gactcgcgga catcaattct gcagccctcc ccggatttgc tctcacgacg gatccgtgct
     3241 acacaagaaa cctcttgaac gccatgattc gtgtcatcaa agccaaagtt cacaatctca
     3301 aggatctgac ggtctcgatc ccgcggaaca gtttgaccgt catcaccggt gtcagcggct
     3361 ccggcaagtc ttcgctggct tttgacacca ttcacgccga aggtcagcgc cgctacatgg
     3421 aatcgctgtc atcttacgcg cgccagtttc tgcagcagat ggagaagcct gacgtcgaag
     3481 cgatcgaggg attgtcgcca accatcgcga ttcagcagaa atcgacgtca cgaaatcccc
     3541 gttcgacggt cgggaccatc accgaggtc
//
LOCUS       DAORHD010000393         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29115_length_3566_cov_3.267730, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000393 DAORHD010000000
VERSION     DAORHD010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29115_length_3566_cov_3.267730"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1269)
                     /locus_tag="PKO06_09360"
     CDS             complement(277..1269)
                     /locus_tag="PKO06_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV69893.1"
                     /translation="MTESNRAPILSVSGLCKRFPLERGLLRSLTDWWRGNEPESVEAL
                     SQVDLQVFPQETVGILGESGSGKSTLARVLMGLYAPDAGRATFLERDLFASDPLIRLQ
                     NLRQMQMVFQDPFGSLDPRMTVRALLEEPLRVHRTFPRREWADRLVRGLAEVGLEPEA
                     LDRYPAEFSGGQRQRIGICRALILDPSLLIADEAVSALDVSVQAQILELLFRLKSVRQ
                     LSLLFISHDVAVVRQLADRILVLYQGRLVESLPADCLLQDATHPYTRRLLGAALELRE
                     AGQAVPEAAGMAVKDDGCPCRSLCRAFQPVCERRPVLEELHPGHRVACWRPTSL"
     gene            complement(1262..2233)
                     /locus_tag="PKO06_09365"
     CDS             complement(1262..2233)
                     /locus_tag="PKO06_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69894.1"
                     /translation="MLLQIADVSLTLRSSDGRCLPVLEQVSLDLDCGQCLGVAGESGS
                     GKSVLALGLMGLLPRSSIAALEGTMIFQGRDLMRLAEAERRSLRGQALAMVFQEPMSA
                     MNPLLTLEEQVGETVAAHVAGVSSSEIRRRVVQALKRSGFAEPERFLASYPHQLSGGM
                     RQRAMLAMALVLEPQLIIADEPTTALDAALQVQLLKELRALVREQRHAMMFISHDLGV
                     IRSIADRLAVMYAGVIVELGDVATVLERPAHPYTSALIRSMPRLVQERRLPQPIPGHL
                     PHPDRKPAGCVFSDRCPRAQDLCRRERPSVSTLAGGQQVRCRFPEHD"
     gene            complement(2281..>3566)
                     /locus_tag="PKO06_09370"
     CDS             complement(2281..>3566)
                     /locus_tag="PKO06_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV69895.1"
                     /translation="KDIVAVEHSADLAKTTVIFPRNSRRYLDLLCLGLVPKHLLASHT
                     PSDPVWVPATGATMLWNELPAASQTAKAARYSFRQWPCGLGRYRITDLKLGYYLHLEP
                     MPLIAAEQNSVEPQEPLPPLPYRHVVARYYYGPEELVNDMRSQTLDWAIIPSEFAARL
                     EELRIPGWNFHRQENPGYTMLGFQTATGPWNLREVREAIDHAIDRKEILALMPIEGVT
                     AGVPPLMPDINRVGLEMRTYDPGKARDLLERAGLRDTNGDGVREFAGQPLVMTILVNS
                     DNLIRKMLAEGLVPMLKKVGLDARIEAVDWSEMIEKRLMNGTFSTFVLGYYAPADGNW
                     INLWHSRPPVGEKLNFTSFAHEELDRVLEAMDRAVPAERPASGAEQIARLLYQHVPGV
                     FLFCPRDVTASRQGLPAGVPNRDWLTNVASTTISH"
BASE COUNT          774 a         1136 c         1032 g          624 t
ORIGIN      
        1 ccctgcgtaa acgttggctc gctcttgcag ccgcgtttgt ctggctgttg ctggatcctg
       61 ggcaaacccg ggggaaagga gcaacagcca gacaaacgcg gctgcaagag cgagccaacg
      121 tttacgcagg ggccacgcca agctcatgaa aagacctcca acggttcctg aattgcatgc
      181 aaggggtcga ttataccgaa gcccccgggg taagtcaacg aaaacggggt accccgccga
      241 aacacatgaa tcgctgaaaa atggcttctg gtgaggctaa agagaggtcg gccgccagca
      301 ggccacgcga tgaccggggt gcaactcctc cagaaccggt cgcctttcgc agaccggttg
      361 aaacgcccgg cacaagctgc ggcaggggca tccgtcgtcc ttgaccgcca ttccggcggc
      421 ctcgggcacg gcctgaccgg cttcgcgcag ctcaagcgct gcccccagta accgtcgggt
      481 atacggatgc gtggcatcct gcagcaggca gtccgccggc aaactctcga ccagacggcc
      541 ctgatacaac accaggatcc gatcggccaa ctgccgcacc acggcgacgt catgtgagat
      601 aaacaacagc gacagttgcc gcaccgactt caaccggaac aggagttcca gaatctgcgc
      661 ctgcaccgac acgtcgagag ccgaaacggc ttcatcagcg atcaacagag atgggtcgag
      721 aatcagagct cgacagatgc cgatgcgctg ccgctgcccc ccgctgaact cggccggata
      781 tcgatcgagc gcttcgggct ccaacccgac ctcagcgaga ccgcgtacca gtcgatcggc
      841 ccattcgcga cgcggaaacg tccgatgcac acgcagcggt tcttccagca gggctctcac
      901 cgtcatgcgc ggatcgagcg agccaaacgg gtcctgaaac accatctgca tctggcgaag
      961 gttctgcagt cgaatcaacg ggtctgaggc aaagagatcc cgctccagaa aggtggcccg
     1021 gccggcatca ggtgcataca gccccatcaa cacccgtgcc agcgttgact tgccagaacc
     1081 gctctctcca agaattccga ctgtttcctg ggggaacacc tgcagatcga cctgtgagag
     1141 cgcttcgacg ctctcgggtt cattcccacg ccaccagtcc gtcaacgagc gcaacaggcc
     1201 ccgttcgagc ggaaaccgct tgcacagacc ggaaacgctc agaatgggag ctctgtttga
     1261 ttcagtcatg ttcagggaat cggcagcgta cctgctgacc tccagcaaga gtgcttacag
     1321 atgggcgttc tcggcggcac agatcctgcg cacgcggaca gcgatccgaa aacacgcacc
     1381 ccgcgggctt gcgatccgga tgcggcagat gaccggggat cggctgtggc aagcgccgtt
     1441 cctgaaccag acgaggcatg gatcgaatca gcgccgaagt atacgggtgt gccggacgtt
     1501 cgagcaccgt ggcgacatcg cccagttcca caatgactcc ggcatacatc accgccaggc
     1561 gatccgcgat cgatcgaatc acccccagat cgtgactgat gaacatcatc gcatgccgtt
     1621 gttcgcgcac cagggcgcgc aactccttga gcaattgaac ctgcagggcc gcatcgagcg
     1681 cggtggttgg ctcatccgcg atgatcaact gtggttcgag caccagggcc atcgccagca
     1741 tggcgcgctg gcgcatgccg ccgctgagct gatggggata actcgccaga aagcgctcgg
     1801 gctcagcaaa tcccgagcgt ttcagcgcct gtaccacgcg gcgacgaatc tcggaagagg
     1861 agacgcctgc aacgtgagct gccacggtct cgccgacctg ttcttccagg gtcaacagag
     1921 ggttcatcgc cgacataggt tcctgaaaca ccatggccag cgcctggcca cgcagagaac
     1981 gtctctcagc ttctgccagg cgcatcagat cgcgcccctg gaagatcatc gttccttcga
     2041 gcgcggcaat cgacgaccgc ggcagcaacc ccatgaggcc gagtgccagc accgacttgc
     2101 cagaacccga ttccccggca acgccaaggc actgcccgca gtcgaggtcg agtgaaacct
     2161 gctccaatac cggcaaacag cggccgtcac tcgaacgcag cgtcagggag acgtcagcga
     2221 tctgcagcag catcactcct ccggggggaa cgggtgcacg atgtggtcat ctttcgaaac
     2281 tcagtgagaa atcgtcgtcg aggcgacgtt ggtcagccag tcacgattgg gaacccctgc
     2341 cggcagaccc tgccgcgaag ccgtcacatc ccgcgggcag aacagaaaca cgcccggcac
     2401 gtgctggtac agcaaccggg cgatctgctc cgctcctgag gcaggtcgtt ccgcgggaac
     2461 ggcacgatcc atggcttcga gaacccggtc gagttcctcg tgagcaaagc tcgtgaagtt
     2521 gagcttctcg cccaccgggg gccgtgaatg ccagaggttg atccagttgc catccgcggg
     2581 cgcatagtag cccaggacaa acgtcgagaa cgtgccattc atcaggcgct tttcgatcat
     2641 ctcggaccaa tcgacggcct cgatgcgagc gtccaggcca actttcttca acatcggcac
     2701 caatccttcg gccagcatct tgcgaatcag attgtccgaa ttgaccagaa tcgtcatcac
     2761 caacggctgt cccgcgaact cgcgcacccc atcgccgttc gtgtcacgaa gtccggcgcg
     2821 ctcgagcaga tcgcgcgctt tgcccggatc ataggtgcgc atctcgagcc cgacccggtt
     2881 gatgtccggc atcagcggag gcacgcccgc cgtcacccct tcgattggca taagtgccaa
     2941 aatctcttta cggtcgatgg catgatcgat tgcttcacgc acttcgcgca ggttccaggg
     3001 cccggtcgcc gtctgaaatc ccagcattgt gtaacccggg ttctcctgtc gatggaagtt
     3061 ccagcctgga atccgcaatt cttcgagacg agcggcaaac tctgacggaa tgatcgccca
     3121 gtccagcgtc tgactgcgca tgtcgttcac cagttcctcg ggaccatagt agtaacgagc
     3181 cacgacatgc cggtagggaa gaggcggcag tggctcttgt ggttcgacgg aattctgttc
     3241 cgcggcgatc agtggcatcg gctcgagatg cagataatag ccgagtttga gatcggtgat
     3301 gcgataccgc ccaaggccgc acggccactg tctgaaactg tatcgcgcgg ctttggcggt
     3361 ctgactggct gccggcaatt cgttccacag catcgtcgct ccggtcgccg gcacccacac
     3421 cggatcagat ggtgtatgac ttgccaacaa gtgcttcggt acaagtccaa gacacagcag
     3481 atcgagatac cgacgcgaat tgcgtggaaa gatcaccgtg gtcttcgcga gatccgccga
     3541 gtgctcgact gcgacaatat ctttgg
//
LOCUS       DAORHD010000394         3560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29187_length_3560_cov_3.567475, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000394 DAORHD010000000
VERSION     DAORHD010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29187_length_3560_cov_3.567475"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PKO06_09375"
     CDS             <1..1597
                     /locus_tag="PKO06_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69896.1"
                     /translation="LGIIVGLAVSHRPTLGVMVVMLIPILWPMSRLRSWVSSSALTGV
                     LLGLLLGLAPLVDLYQRLQNPERQLIDPLIGRGFEGFWSFFTAADFRKAVGVFGPLEL
                     LARAKHWGWLVAEQGTPALLLLPLFTLRQLPLSREAMALIWIMLFNSGFILNYNAYDA
                     HTMLMPSFLALTGLSALFLAKVDGPRKKWLTPAFLFLVANSLFVNGYTREPAERQCES
                     FTYRLTALMPQGSTIFVNDDLEFRPLLYLRQTRQFRSDLSLHLIDAFQEQDLPAIRGD
                     FARGPVFSTLVFPESLAPLLNREYHLVPWGYAWRVLPANISPTPAPDVRWGREVSLAG
                     SATFQVADHCAILKHRVPGQDDGPAILPGDVLQYEYRLASAGPLADSLVVAPFLVSLD
                     GALPAEKGVVLGHDLHIVGTGSCRSSTWYQERYHALYQRRQLIVPEFLRPGRYDVRLL
                     LTTTDKLAQSHLLLERLPRANLLGEQGETELFRLQNGLGLRPLVDAALTPTWNRLLES
                     GPAWVNASSPIVLGSFEIPASPR"
     gene            complement(1607..>3560)
                     /locus_tag="PKO06_09380"
     CDS             complement(1607..>3560)
                     /locus_tag="PKO06_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69897.1"
                     /translation="ACPTGDGHVHRLLSVNPDRSRSLFEQPWFTSLVDTFHVFSEPGY
                     LDAQLVTEGIWTHLTESGFHISLVIPLFAAQERMGAILLLGLPEQKRMAVLRETLRPL
                     SQIVGLLLKNILLVEEQDLMLDARARGLRESESRLSHAQRSAGFGVWELDTSHDRLIL
                     TSSAWDILRVPPSDAPDLKTLLEPVIMAEDFPLLDAALQRTLQLRQPLDVEFRIQQSG
                     ERCRWLRLAGGWSNERDGEKSQLFGVFQDVTERRDIEERLRHSEKLEAIGQLAGTIAH
                     DFNNMLSGILGYADLLMQELPAGSHRQITEKIIGAAQRSADVARKLLLISRKEKVKMG
                     TCDLHQILHDVIGLLGHSLDKRITVSEKLQAGGATTFGDQSQLQNVFLNLALNARDAM
                     PDGGMLTISTADVRVTINTPISGPLPVAPGRYIAVSVSDTGCGMSAQVKKHLFQPFFT
                     TKESGKGTGLGLTAVLSTVSQHQGAVTYESEAGTGTTFTVYLPFTSAAEVRVANDVEP
                     RAVVVKGTVLVVDDEEIVRKLTTQMMKKLGYSVETCEDGQAAVEICSDHGQRFQAVIL
                     DIAMPRLNGRDAFVAIRQRHPELPVLFCSGYIMDTDLSDLLSQPAVGFLSKPFRQVDL
                     AVAIAELLHRADPHQAISAKVPAGQS"
BASE COUNT          657 a         1066 c         1028 g          809 t
ORIGIN      
        1 ccttgggatc attgttggtc tggcggtgtc gcatcgtcca accctcgggg tcatggttgt
       61 gatgctgatt cccattctct ggcccatgtc gcggttgcgt tcgtgggtct cctcgtcggc
      121 tcttactggg gtccttctcg gtctgctcct cggactggct cccctggtcg atctctatca
      181 acgcctgcaa aaccccgaac gacagctgat cgacccgctg attgggcgag gctttgaagg
      241 attctggtcg ttctttaccg cggccgattt tcgcaaggct gtcggggtgt tcggtccgct
      301 cgagcttctg gcgagagcga aacactgggg ttggctcgtt gccgagcagg ggaccccggc
      361 actgctgttg ctcccgctct tcaccctgcg ccagcttccc ttgtcgcgcg aggcgatggc
      421 tctcatctgg atcatgttgt tcaattcagg gttcatcctc aactacaacg cgtacgatgc
      481 tcacaccatg ctgatgccca gttttctcgc tctcaccggg ctcagcgcgc tgtttctggc
      541 gaaagtcgac ggaccccgaa aaaagtggct gacccccgct tttctgtttc tggtcgcgaa
      601 tagtctgttc gtcaacgggt acacgcgcga accggcagag cggcagtgtg aatcctttac
      661 gtatcgcttg acagccctca tgcctcaggg ctcgaccatc ttcgttaatg acgacctcga
      721 gtttcgccca ctgttgtatc tgcgccagac tcgtcagttc cgctccgatc tttccctcca
      781 tctcatcgat gctttccagg aacaggatct ccccgccatt cgcggcgact ttgcccgcgg
      841 cccggtgttc tcgaccctcg tgtttcctga atctctggct ccgttgttga atcgggaata
      901 tcatctcgtt ccgtggggat atgcctggcg tgtgctgccg gccaacattt cgccgacacc
      961 cgcccccgac gtccggtggg ggcgcgaagt gtcgcttgct ggttccgcca cgttccaggt
     1021 ggcagatcac tgcgccattc tgaagcatcg agttccgggg caggatgacg gcccggcgat
     1081 tctgccggga gatgtgctgc agtatgagta ccgtctggca tcagccgggc cgctggccga
     1141 ttcgctcgtg gtcgctccgt ttctcgtgag ccttgacggg gcattgccgg ccgagaaagg
     1201 ggttgtgctt ggtcacgacc tgcatatcgt gggtaccggt tcctgtcgca gcagcacttg
     1261 gtaccaagag cggtatcacg ccctgtatca acggcgtcaa cttattgttc ccgagtttct
     1321 tcgccccgga aggtatgacg ttcgtctgct gctgaccacg accgacaagc tggcgcaatc
     1381 gcacctgctg ctcgaacggc tgcctcgcgc gaacctgctg ggcgagcagg gcgaaaccga
     1441 actgtttcgc ctgcagaacg gtttgggcct gcgacctctg gtcgatgccg ctctgacccc
     1501 cacctggaac cgcctgctcg agtcggggcc cgcctgggtg aatgcttctt cgcccatcgt
     1561 gctgggttca tttgagattc cggcgtcgcc gcgttaatcg ctggtgtcag ctctgaccgg
     1621 ctgggacttt cgcggaaatt gcctgatgtg gatcggcacg atgtaacagt tcggcgattg
     1681 ccacggccag atctacctga cgaaacggtt tcgaaagaaa gcctacggcg ggctggctga
     1741 gaagatcaga caggtccgtg tccatgatgt atcctgaaca gaacagcacc ggaagctcgg
     1801 gatgacgttg gcgaatggcg acaaaggcat cgcgtccgtt caggcgtggc atggcgatat
     1861 cgagtatgac tgcctgaaag cgctggccgt gatctgaaca gatctcaacc gccgcctgac
     1921 cgtcctcgca ggtttcaacc gagtatccaa gcttcttcat catctgcgtg gtcaacttgc
     1981 gaacgatctc ctcgtcgtcg acaaccagga cggttccctt gacgacgaca gccctgggct
     2041 caacgtcatt ggcgacccga acttcggcag cgctggtgaa cggcagatac acggtaaacg
     2101 tcgttcctgt acctgcctca ctctcgtatg tcaccgcccc ctgatgctgt gacaccgtgc
     2161 tgagaaccgc agtgagacca agacccgttc ctttgcccga ttccttcgtc gtgaagaagg
     2221 gctgaaacag atgtttcttc acctgtgccg acattccgca tccggtatca gaaaccgaca
     2281 ccgcgatgta gcgtcccggc gcgacgggca aaggcccgga aatcggagta ttgatggtca
     2341 cccgaacatc cgcggtgctg atcgtcagca ttccgccgtc aggcatggca tcgcgggcgt
     2401 tcagggcaag attgagaaac acgttctgca actggctttg atcgccgaag gtggttgctc
     2461 cgcccgcctg cagtttttcg gagacggtga tgcgtttgtc gagtgaatgt cccagcagac
     2521 cgatgacgtc gtgaagaatc tggtgaagat cgcacgtgcc catcttgacc ttttctttgc
     2581 gcgaaatcag cagcagtttg cgcgccacgt ccgctgaacg ctgggccgcc ccgatgattt
     2641 tctccgtgat ctgtcgatga gacccggcag gcaattcctg catcagcagg tcagcatacc
     2701 ccagaattcc tgacagcatg ttgttgaaat cgtgagcgat ggtgccggca agctggccga
     2761 tagcctcaag tttctcgctg tggcgtaacc gctcctcgat gtctcgacgt tccgtgacat
     2821 cttgaaagac accgaacagc tgacttttct cgccgtccct ctcattgctc cagcccccgg
     2881 ccagacgcag ccagcgacag cgttcaccac tctgctgaat ccgaaactcg acatccagtg
     2941 gttgtcgcag ctgcagtgtc cgttgcagag cggcatcgag taaagggaag tcttctgcca
     3001 tgatgacggg ctcaagcagt gttttcaggt caggcgcgtc gcttggcggc acgcgtagaa
     3061 tgtcccaggc tgatgatgtc aggatcagcc tgtcatggga cgtatcgagt tcccagacac
     3121 cgaagcccgc gctgcgttgg gcatgagaaa gccgcgactc actctctcgc agaccacgtg
     3181 cccgggcatc cagcatcaga tcctgttctt ccaccagcag gatgttcttc agcagcaggc
     3241 cgacaatctg actcagcggt cgcagcgttt cacgcagaac cgccattctc ttctgctccg
     3301 ggagtccgag gagtagaatc gctcccatgc gttcctgagc agcaaagaga ggtatgacaa
     3361 gagaaatgtg aaacccggat tcggtgagat gggtccagat accttccgtt accagctggg
     3421 cgtcgagata gcccggctca gagaagacgt gaaacgtgtc gacgagtgac gtgaaccagg
     3481 gttgctcgaa gagggagcgg ctgcggtcgg gattgaccga caggagtcga tgcacgtgac
     3541 cgtcaccggt tgggcaggca
//
LOCUS       DAORHD010000395         3555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29252_length_3555_cov_3.842571, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000395 DAORHD010000000
VERSION     DAORHD010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29252_length_3555_cov_3.842571"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="PKO06_09385"
     CDS             complement(<1..102)
                     /locus_tag="PKO06_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007390591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV69898.1"
                     /translation="MKILPRLVIGKLRPRYPLIQGGMAIKVSTGRLAG"
     gene            complement(185..427)
                     /locus_tag="PKO06_09390"
     CDS             complement(185..427)
                     /locus_tag="PKO06_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012954527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV69899.1"
                     /translation="MSQNYFEEVKKIVVEKLQVDEKQVTMEASFIEDLGADSLDTVEL
                     VMDLEEHFGIEIPDEEAEKLKTVKDAVDYITKKKGN"
     gene            complement(457..1215)
                     /gene="fabG"
                     /locus_tag="PKO06_09395"
     CDS             complement(457..1215)
                     /gene="fabG"
                     /locus_tag="PKO06_09395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HNV69900.1"
                     /translation="MTQAETTAPTRVALITGSARGIGKEIALTLGQNGYAVMLSDVNA
                     EQLEKTVGEFRSQGIKTGSFVANVSKSAEAEALVEETIKTFGRIDLLVNNAGITKDGL
                     LLRMTEEQWDAVINVNLKGTFLLSKAVCRPMLKQKSGCVVNIASVIGLMGNAGQTNYA
                     ASKAGIIAFTKSYAKEFARKGIRANAVAPGFIMTEMTSVLPEQVKEEMLRSIPMGTLG
                     SPRDVANAVLFLASDQAAYITGQVLTVDGGMVMS"
     gene            complement(1311..2252)
                     /gene="fabD"
                     /locus_tag="PKO06_09400"
     CDS             complement(1311..2252)
                     /gene="fabD"
                     /locus_tag="PKO06_09400"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNV69901.1"
                     /translation="MNALVFPGQGSQKIGMARVMVETHPWAAAMARRADEILGHSISS
                     VCFTGPEESLKKTINTQPALFLTEAILTEALRRQQIPFQAVAGHSLGEYSALYAAGVA
                     DFEDLLRLVQTRARAMETACPTGEGAMAAILMLDRQKIAAICQEVAPIGVCVMANLNA
                     PGQVVISGAAAAVTKAGELAKAQGAKRVVMLEVSGPFHSPLMTKARTELEQAIRDITF
                     RTATVPVYTNVDAAPTTDANQIKNKLLEQLTGTVRWEDAIQAMFAAGFRRFIEVGPGK
                     VLCGLIKKIAPEAETAQFEDPTTLAAIQTPASAAAQA"
     gene            complement(2257..3237)
                     /gene="fabK"
                     /locus_tag="PKO06_09405"
     CDS             complement(2257..3237)
                     /gene="fabK"
                     /locus_tag="PKO06_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HNV69902.1"
                     /translation="MIETQITRLLGIRYPVLQGGMAWVSSASLVAAVSNAGGLGVLGA
                     GPMDPDTLTREIHQIKSLTNKPFGVNIMLMMPPAPELVKICVNEKVPVVTTGAGNPGP
                     YVKALKDAGSRVIPVISSVALAKRLERSGVDAIIAEGNESGGHIGEISTMVLIPQVAD
                     AVKIPVIAAGGIADGRGILAALSLGAQGVQMGTRFILAEECHVHENYKKVVANAKDRD
                     TCITGLSTGHPVRVIRNKIAKYYLEREHAGATPQELDDIARGTLRKAVHEGDVDNGSV
                     MAGQICGLIDRVETAAEIFARMDREFHQAYEQVSGLFNANARSHSSSTKD"
     gene            complement(3367..>3555)
                     /locus_tag="PKO06_09410"
     CDS             complement(3367..>3555)
                     /locus_tag="PKO06_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69903.1"
                     /translation="APEYSGAPLLGIAGGSIVCHGKSKAPVIANALHLAAQFASTDVI
                     GLIAQRLSQNAAGVSISQ"
BASE COUNT          685 a          994 c         1085 g          791 t
ORIGIN      
        1 accggccaac cggcccgtag agactttgat tgccatgccg ccctgaatga gcggatagcg
       61 ggggcgcagc ttgccgatga ccaaccgggg aagaattttc acgagacgaa cctgtttgag
      121 acatcgcccc gccggaacag cctttcagcc tgaccggcgg ggagcgatga ttgcaggaaa
      181 aaggtcagtt gcccttcttc ttggtgatgt agtcgacagc gtccttgacg gtcttgagct
      241 tttccgcttc ttcgtcggga atctcgattc cgaagtgctc ttcgagatcc atgaccagct
      301 cgacggtgtc gaggctatcg gcgccgaggt cttcaatgaa gctggcttcc atggtgacct
      361 gcttttcgtc cacctgcagc ttctcgacca cgatcttttt gacttcttcg aaatagttct
      421 ggctcattgt tcaactcctc gttagagaat cttgtattac gacattacca tgccgccgtc
      481 gacggtcagg acttgccctg tgatatacgc cgcctgatca gacgccagaa agagaacggc
      541 gttggcgaca tcgcggggtg atccgagcgt tcccatgggg atcgagcgga gcatttcttc
      601 cttgacctgc tctggcagga cgctggtcat ctcggtcatg atgaaaccgg gggccacggc
      661 attggcccga atacccttgc gggcaaattc cttggcgtag cttttggtga aagcgatgat
      721 gccggccttg ctcgcggcgt agttggtttg tccggcattg cccatgagac cgatcactga
      781 agcaatattg acgacgcaac cgctcttctg cttgagcatt gggcgacaga ccgctttcga
      841 cagcaggaac gtccctttca ggttcacgtt gatgaccgca tcccactgtt cttcggtcat
      901 gcgcagcagc agcccgtctt tggtgatacc ggcgttattg accaacagat cgatacggcc
      961 gaaggtcttg atcgtctctt caaccagggc ttcagcttcg gcgctcttgg agacgttggc
     1021 cacaaagctg ccagtcttga tgccctgcga acggaactcg ccaaccgtct tctcgagctg
     1081 ttcggcgttg acgtcggaca acatgacggc atacccattc tgaccgagag tcagggcgat
     1141 ttccttcccg atgcctcggg ctgagccggt aatcagggct acacgggttg gagcggtggt
     1201 ttctgcttgt gtcatgtgtg agtgttcctg agagggaatg agattccgtg gcgcatttat
     1261 agctgaatgt gggcccggtg tcaaggatca gaggcgaaaa ttccggctcg tcatgcctgt
     1321 gccgcggcgg aagccggtgt ctggattgcc gcgagcgtgg tcgggtcttc gaactgagcg
     1381 gtttccgctt ctggggcgat tttcttgatc agtccacaga gcaccttgcc tggcccgact
     1441 tcgatgaacc ggcggaaccc ggcagcgaac atggcttgaa tggcgtcttc ccagcgaacc
     1501 gtgcccgtca gttgttcgag cagtttgttt ttgatctggt tcgcgtcggt ggttggcgcg
     1561 gcatcgacgt tggtatacac cggtaccgtg gccgtacgga aggtgatgtc gcgaatcgcc
     1621 tgctcgagtt cggtgcgggc tttggtcatg agtggcgaat gaaacgggcc ggaaacttcg
     1681 agcatgacga cgcgcttggc cccttgagcc ttggccagtt cgccggcttt ggtcacggcg
     1741 gcagcggctc cggagatgac gacctggccg ggagcgttca ggttggccat gacacacacg
     1801 cctatcggtg cgacttcctg gcagatggcg gcgatcttct ggcggtcgag catcaggatg
     1861 gctgccatgg ccccttcacc cgtcgggcag gccgtttcca tggcgcgggc tctcgtctga
     1921 accaggcgca gcagatcttc aaaatcggca acaccggccg catagagcgc ggaatactcg
     1981 cccagactgt gcccggcaac cgcctgaaag ggaatctgct gtcggcgcag ggcttcggta
     2041 aggatggctt cggtgaggaa cagcgctggt tgcgtgttga tcgttttctt cagggactct
     2101 tccggtccgg tgaagcagac tgaactgatc gagtgaccaa gaatctcgtc ggcgcggcga
     2161 gccattgccg cggcccaggg atgtgtttcg accatgacgc gggccatgcc gattttctgt
     2221 gaaccctgac caggaaaaac cagtgcgttc atggaattag tcctttgtcg aagaagagtg
     2281 ggagcgagca ttcgcgttga acaacccgga cacctgctca taggcctggt ggaactcgcg
     2341 atccatgcgg gcgaagattt cggccgccgt ttcgacgcga tcgatgagcc cgcagatctg
     2401 gccggccatg accgaaccat tgtcaacgtc accttcgtga acggctttgc gcagggtgcc
     2461 gcgggcaatg tcgtcgagtt cctgaggggt tgcaccggcg tgttcccgtt cgaggtagta
     2521 tttcgcaatc ttgttgcgaa tcactcgaac cgggtgcccg gtcgacaggc cggtgatgca
     2581 ggtgtcgcga tcttttgcgt tggcgacgac cttcttatag ttttcgtgaa catgacattc
     2641 ttcggccaga atgaagcgag tgcccatctg cactccctga gctccaagcg acagagccgc
     2701 cagaatccca cgcccgtcgg cgatgccgcc ggcagcaatg acgggaatct tgactgcatc
     2761 agcgacctgc ggaatcagaa ccatggtgga aatttccccg atatgaccgc ccgactcatt
     2821 cccttcggcg atgatggcat cgacgcctga gcgctcaagg cgcttggcca gagccacgga
     2881 cgagatcacc ggaatcaccc ggctgccggc atccttcaac gccttgacat aggggcctgg
     2941 gtttcccgct ccggtcgtga cgacgggaac cttttcgttc acgcaaattt tgactaactc
     3001 gggtgcgggc ggcatcatca gcatgatgtt gaccccgaac ggtttgttcg tcagcgattt
     3061 gatctgatga atttctcggg tcagggtgtc cgggtccatg gggcctgctc cgagaactcc
     3121 gagaccgccg gcattgctga cggcagcgac aagactggcc gaactgaccc aggccatgcc
     3181 tccctgaaga accggatacc gaatgccgag aagacgggta atctgcgttt caatcatgat
     3241 actggactcc ctcgcaccca tactgcatcg ataagatcga gacctgtgga acggatgccg
     3301 ggggtgcgaa caaggaaagg tatgatgcga tgggaaccgt attcacacgg tgaccaggcc
     3361 tgatgcttac tgcgaaatgg aaacaccggc agcattctgg gaaagccgct gcgcgatgag
     3421 tccgatgacg tccgtgctgg cgaactgagc cgccaggtgc agggcgttgg cgatgaccgg
     3481 ggcttttgat ttgccgtgac agacgataga accgccggcg attccgagca gcggagctcc
     3541 gctgtattcg ggggc
//
LOCUS       DAORHD010000396         3550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29320_length_3550_cov_3.633190, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000396 DAORHD010000000
VERSION     DAORHD010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29320_length_3550_cov_3.633190"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKO06_09415"
     CDS             <1..914
                     /locus_tag="PKO06_09415"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNV69904.1"
                     /translation="RLITEMPQSWRSHLVFLGSAAILSREQKRLGHSPLAACAPLQPR
                     ANHLMLLDTGSGADCPPGVVCDNGGREALNALQAAHDLCRRGDCLGMITGPIGKKAIR
                     LAGSSFSGHTDMLEAWTGARATRMAMIHGRFRVVMSTLHVPYRSVPALLTESCVYETI
                     RLTHEAFRTRQRPFPRIAVAGLNPHAGEDGLFGDEERCSIIPAMERFKSQNPHLSGPF
                     PADSLYKRELRRSTDAFVAHTHDQGLIAIKTLGGLSCVNVTLGLPYIRTSVGHGTAYD
                     IAGKGCADAAGLGAACRAAFRLAGVRP"
     gene            1115..2686
                     /locus_tag="PKO06_09420"
     CDS             1115..2686
                     /locus_tag="PKO06_09420"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV69905.1"
                     /translation="MDLNQPYSRVPRPERFRFLRRKLRELQLVYAIDRVTDRHQHLSG
                     LARYTLRVTRRFFRAKAVALLLADQDNRQQQGFFSLGFSHDGLNQQHRMYVKDLLHRH
                     LADESEGTILVAPGQALPEGIAHLALRSFAVADGLRGCLLIANLAKRPPSTEDLHYLK
                     AIISQIDNAIIHGRFLEKFHQTSHLVNKQTREIDFMYEISLSIGRDEDFVSMTRRILE
                     SALQLVEVNRCSLMLYDAPTDTLQTELVVGAPHLKTGFRLRLGEGLAGTALKEGKAIL
                     AVRGSKDQRFVSVAGETTPQGTVANMACVPLISDTTKLGVLNFTSTDPKYEVTAQELD
                     TLGVVANLIVLAYQRQRLYQLSIKDELTGLYSFRFFSQRLGEELTRASRYKSCFSLVL
                     FDIDHFKKLNDSWGHPFGNIVLKAVAKVLQDSIRQGIDFPARFGGEEFALLLPETSAA
                     NALILAERIRQRVGELLFDEKGNIVRTSISGGIAAFPICVNAEQILEKADQSLYNAKS
                     SGRNRIVISDEPPTL"
     gene            complement(2769..>3550)
                     /locus_tag="PKO06_09425"
     CDS             complement(2769..>3550)
                     /locus_tag="PKO06_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69906.1"
                     /translation="LPGMEKKDRNEQVGVTMEGLTGLTRTFAAYPLGKGLFSTSFGVI
                     WNKITKRDNNRLQNGEDADYWQFPMAITYSGEGFEGALQLPFESYDVYAPLTYNFRDG
                     TDSGMGDAALRLKFSSQNDNMASCIGVGAIFPTNDRRIGNTDNDNAWEVFGGVSTKKK
                     EGGNLHLNGGYQASAGNTSHEGVFVNVGFEYAANPSFTFMGEINSYNRVNDGRATDLT
                     LSMRYHVKPGMSLTLAAPISLQNEMFFGYDYRLQGQLQYHY"
BASE COUNT          718 a         1095 c          958 g          779 t
ORIGIN      
        1 cgcgcctgat caccgagatg cctcaatcct ggcgatcgca tcttgtcttt ctcggatccg
       61 ctgccattct ttctcgcgaa caaaagcgac tcggacattc gccactcgcg gcctgtgccc
      121 cattgcagcc gcgagcgaac cacttgatgc tgctcgacac gggttcagga gctgactgtc
      181 ccccgggagt cgtctgtgac aatggcgggc gcgaagcgtt gaacgccctg caagcggcgc
      241 acgatctgtg tcgccgaggg gactgcctcg gcatgattac cggcccgatc gggaaaaagg
      301 ccattcggct tgctggctcc tcattttccg gccataccga catgcttgaa gcgtggacag
      361 gcgcgagggc cacccgcatg gccatgattc atggacgttt tcgcgtagtc atgtccacgc
      421 tgcacgtccc ctatcgttcc gtaccagctt tactcacaga atcatgtgtc tacgagacca
      481 ttaggctgac ccacgaagcg tttcgcacgc ggcagcgccc ctttccgcgc attgccgttg
      541 ccggcttgaa cccgcatgcc ggcgaagacg gtttgttcgg cgacgaggaa cgatgcagca
      601 tcatccccgc gatggaacgt ttcaaatccc agaatcctca tctcagtggt ccgttccctg
      661 cagacagctt gtacaaacgt gagttgcgcc gttccacgga cgcgttcgtc gcgcacaccc
      721 atgatcaggg gctcattgcc atcaagaccc tcggcggact gtcgtgcgtc aatgtcacgc
      781 tggggctgcc ctacattcgc acctcggtcg ggcatggcac ggcgtatgat attgccggca
      841 aggggtgtgc cgatgccgca gggctgggcg cagcctgtcg cgctgcgttt cgcctcgccg
      901 gcgtgcgacc gtgacccggc gcggcgcacg tgtttgattc gcaacgttaa gttccggttc
      961 accagccctt cggtctgtca ggcgaacaga ttctggcacc atttgggggg ggaagctgat
     1021 tcagacactt gcccccctct gacaattgat ttatcatatg ggcagtctcg agatcagcag
     1081 agcagcgtag cgctttccgg caaggagacc cagcgtggac ctgaatcagc cttattcccg
     1141 ggttccccgc cctgaacgat ttcgttttct ccgtcgaaag ctgcgcgaac tgcaactcgt
     1201 ctatgcgatc gatcgggtca cggatcgcca ccagcacctt tccggtctcg cccgctacac
     1261 gctgcgggta accaggcgct tctttcgtgc caaagccgta gccctgctgc tggccgacca
     1321 ggacaatcgg cagcagcaag gttttttcag cctgggcttc agtcatgatg ggctgaacca
     1381 gcagcaccgg atgtatgtca aagacctgct gcatcgacat ctcgcggacg agtcagaagg
     1441 cacgatcctg gtcgcgcccg ggcaggcact ccctgaaggc atagcccacc tggccttacg
     1501 atcgttcgcc gttgccgacg gactgcgtgg gtgccttctg atcgccaatc tggcgaagcg
     1561 accgccttcg accgaagatc tgcactacct gaaagcgatt atttcccaga tcgacaacgc
     1621 catcattcac ggccgatttc tcgagaagtt ccatcagacc agccatctcg tcaataagca
     1681 gactcgagaa atcgatttca tgtacgagat cagtttgtcg atcggccgcg acgaagattt
     1741 cgtctcgatg acccgccgca ttctcgaaag cgcgctgcaa ctcgtcgagg tgaaccgatg
     1801 ttcgctgatg ttgtatgacg cccccaccga caccctgcaa accgagctcg tcgtcggcgc
     1861 ccctcacctc aaaaccggct ttcggcttcg tctgggcgaa ggtcttgccg gcaccgccct
     1921 caaagagggg aaggccattc tcgcggttcg cggcagcaaa gaccaacgat tcgtgagcgt
     1981 tgccggcgaa acgactccgc aaggtaccgt tgccaacatg gcttgcgtgc ctctgatctc
     2041 ggacaccacg aaactcggcg tcttgaactt cacttcgacc gatccgaagt acgaagtcac
     2101 agcccaggaa ctggacacgc tcggagtggt cgccaatctc atcgttctgg cgtaccagcg
     2161 ccagcgattg taccagctct ccatcaaaga tgagttgacc gggctctatt cctttcgctt
     2221 cttttcccaa cgactcggag aagaactcac acgcgcctcg cggtacaagt cgtgtttcag
     2281 tctggttctg ttcgatatcg atcatttcaa gaaactgaat gattcctggg ggcatccctt
     2341 cggaaacatc gtgctgaagg ccgtggccaa agttcttcag gattccatcc gccagggcat
     2401 cgattttcct gcccggtttg gcggcgaaga gttcgcgctt cttcttcccg agaccagcgc
     2461 cgcgaacgcc ctgatcctgg cggaacgtat tcgtcagcgc gtcggcgaac tgctgttcga
     2521 cgaaaagggg aacatcgtgc gcacgtcaat cagcggcgga atcgctgcct ttcccatctg
     2581 cgtcaacgct gagcagatcc tggaaaaggc cgatcagtcc ttgtacaatg ccaaatcatc
     2641 cggtcgcaat cgtattgtca tcagcgacga accgccgacc ctctgatacc gaggcaacga
     2701 gaaccggccc ggtctttcga ccgggccggt tctcctctgc tgtacctctc ttacgagggg
     2761 tattggtttc agtagtggta ctgcagttgc ccctgcaggc gatagtcata cccaaaaaac
     2821 atctcgttct gcagcgagat cggagccgca agagtcagac tcattcccgg cttgacatga
     2881 tagcgcatgc tgagagtcaa atccgtcgcg cgtccgtcat tcacccgatt gtacgagttg
     2941 atttctccca tgaaggtgaa tgaggggttg gctgcgtatt caaacccgac gttcacgaac
     3001 accccctcgt gagaggtatt accggcgctg gcctgatacc cgccattcaa gtgcagatta
     3061 ccgccttcct tcttcttcgt tgagacgccg ccgaagactt cccaggcatt gtcattgtcg
     3121 gtattgccaa tgcgacgatc gttggtggga aagatcgctc ccacaccgat gcaagaagcc
     3181 atgttgtcat tctgactcga gaacttcaga cgcagagcgg catctcccat tcccgagtcg
     3241 gtaccatcgc gaaagttgta agtcaacgga gcatagacat cgtatgactc gaaaggcagc
     3301 tggagtgcgc cctcaaaccc ttcaccggaa taggtgatcg ccatggggaa ctgccagtag
     3361 tcggcgtctt caccattctg cagacggttg ttgtcgcgtt tggtgatctt gttccagatc
     3421 acgccgaagg aggtcgaaaa gaggcccttg ccaagcggat acgcggcgaa ggttctggtc
     3481 agacccgtca agccttccat cgtcaccccc acctgctcat tgcgatcttt cttttccatg
     3541 ccgggcagca
//
LOCUS       DAORHD010000397         3549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29336_length_3549_cov_3.602461, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000397 DAORHD010000000
VERSION     DAORHD010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29336_length_3549_cov_3.602461"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PKO06_09430"
     CDS             complement(<1..1263)
                     /locus_tag="PKO06_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69907.1"
                     /translation="MIIDPNGFQVRKPTVPLRGPVLVFLTFGLIIASIWGCGSNGGGI
                     GDWQISTPTTGGSGNIQGAVLAPIPGLGNIHARISPSAGAAVDATVYLEERPDLTTKT
                     DTAGKFLLRNVPVGKYHIIAELLVGVTSYRQRSDLVTLGSANTTQELSTPLQLLEARR
                     NAVLFLHNRQTDELITSGITATLWGRSVLPALDGLVSLGPVTTGVWPVRIEGSGFYST
                     TLLVNFTDNRQNNLVYELTPLTAVDRNQAPFVEMGRDFTTMRTYQSGNLTATSLDPEG
                     DVVTYSWEATGGTLSNTQGATTVLTAGDTPGQITIVVTGTDSKGGTGRAVMVVNVISG
                     GALPAPASNLPPNSATEPQPANGSVDMGTNIALRWTGSDPNGDALRYDLLLWPLGSTS
                     SVIATNLQNPAFPLADLTPFTTYFWQVIS"
     gene            complement(1342..2787)
                     /locus_tag="PKO06_09435"
     CDS             complement(1342..2787)
                     /locus_tag="PKO06_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69908.1"
                     /translation="MKRGLTLVEIMVGVILSSIVLGAGFQIWSSARRDITTANLRQNL
                     QHDIRIALDRMGSDFKSMKAGTLTDPVQNGNSYSFTLDRYVADKDGKPTTKTVKVTWE
                     FNEVSHVLSRTESGGDYDGKRIMSTNLISLDVARGSESTLQVKGSAMASVANDSESGR
                     SARFDITMDGERKNTMTGKIEKHTEKTTVFVRDEYYTAITKGKHLKIRELTATESSEL
                     TASKIDAKEGFFAKNLTDEMLAGMSKTELQDLKTKETEARQQAEDNLKKLNDSLGQID
                     AKKSFLWFFSKSTETSKIQDRIIEHDTKAELEQDIKELDEKINKDEKTFIQNSLNGAG
                     LNYADIEKDPTKAKAYKEAYELQIRKRNLEKSIKEMGDKAENAQQKQELQKMVDDMNT
                     QIQSGQIQRAQNEDQKSFEARQAHMAQVLGAAKQMDMTWMDGKEEELKEYEAAKQIID
                     LGQAKLGSVDGRDSAQANITKLDEFINKAKS"
     gene            complement(2809..3312)
                     /locus_tag="PKO06_09440"
     CDS             complement(2809..3312)
                     /locus_tag="PKO06_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69909.1"
                     /translation="MIAMRPMSFIVIRSRGVGMLEILIAAAIIAVAFIPILSAIQFGN
                     RSTVKINNYSKAAKLAQSLIEECKHTPIKKYAEKYKSTPDKTLTDIAEEYYPVSAKEF
                     KEFGKSLKDFKYKAQLKPLRHPDDKDKLREVWIQVEVNWREGDGTTVNQPRTLRIGNA
                     LHNPDSD"
     gene            complement(3309..>3549)
                     /locus_tag="PKO06_09445"
     CDS             complement(3309..>3549)
                     /locus_tag="PKO06_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69910.1"
                     /translation="GCVYTDKGIAVPKNKGLRIVGNWITNVWNKATSSGDIMVEYVGY
                     KTRSSLFSLHPDYGKYDPERYLVTLSPTWESWKVK"
BASE COUNT          664 a          970 c          979 g          936 t
ORIGIN      
        1 actgatgacc tgccagaagt aggtggtgaa cggcgtgaga tcggcaagcg ggaaagccgg
       61 gttctgcaga ttggtggcaa tgaccgaaga tgttgagccg agaggccata acagaagatc
      121 gtaacgcagg gcgtctccgt tcgggtcaga tccggtccat cgcagggcga tattcgtgcc
      181 catgtcaacc gatccatttg cgggctgtgg ctcagtggca gaattgggcg ggaggttgga
      241 tgcaggcgca ggcagagctc cgccactgat gacgtttaca accatcacgg ctctaccggt
      301 tccgcccttg gagtcggttc cggttaccac gatcgtgatc tgacccggtg tatctccagc
      361 tgtgagaacg gtggtcgccc cctgcgtatt gctgagtgtc ccacctgtcg cttcccagga
      421 ataggtcacc acgtcgcctt ccggatcaag cgaagtcgcg gtcaggttgc cgctctgata
      481 ggtgcgcatc gtcgtgaagt ctcgccccat ctcgacgaag ggggcctgat tgcgatcaac
      541 agccgtcagg ggggtcagct cgtagaccag attgttttgt cgattgtcgg taaaattcac
      601 cagcagcgtc gttgaataga agccgcttcc ttcgatcctg accggccaga cgccggtagt
      661 cacggggccg agagatacca gaccgtcaag cgcgggcagg acgctgcgcc cccacagtgt
      721 tgcggtaatg ccgctggtga tcaactcgtc ggtttgccga ttatgcagga acagtacggc
      781 gttgcgtcgg gcttcgagca gctgaagcgg cgtcgacagt tcctgggtcg tgttcgccga
      841 gcccagcgtt accagatcgg atcgctggcg gtacgaggta acgccgacca gcagttcagc
      901 aatgatgtga tattttccaa ctgggacatt tcgcagcagg aactttccgg ccgtatccgt
      961 ttttgtcgtg aggtccggac gttcttcaag atagacggtg gcatcgaccg cggcccccgc
     1021 cgatggactg atccgggcgt gtatgtttcc cagcccggga atgggtgcta gaaccgcgcc
     1081 ctgaatgttc cctgagccgc cggttgtcgg ggtgctgatc tgccagtcgc cgatgcctcc
     1141 gccgttggag ccgcatcccc agatactggc gataatcagg ccgaaggtga gaaataccaa
     1201 aaccggcccc cggagcggga ccgtcggttt ccggacctga aagccgttcg gatcgatgat
     1261 catgaatgaa tcgtttctct gcttctatcg gcagaaaaaa agatcccgtt caccatgaac
     1321 gggatctttg cggaaacgat ctcaggattt tgctttgttg ataaactcgt cgagctttgt
     1381 gatattggct tgagcactgt ctcgcccgtc gacgctgccc agttttgcct gccccagatc
     1441 gataatctgt ttggccgctt catattcttt cagttcttct tccttgccat ccatccaggt
     1501 catgtccatc tgcttggcgg ccccgagcac ctgcgccatg tgggcctggc gggcttcgaa
     1561 tgatttctgg tcttcattct gtgcacgctg aatctgaccg gactggatct gggtgttcat
     1621 gtcgtctacc atcttctgca gttcctgctt ctgctgggca ttttcggcct tgtcgcccat
     1681 ttccttgatg ctcttttcga gattccgctt gcggatctgc agttcatacg cttctttgta
     1741 agctttggcc ttcgtcgggt ctttctcgat gtcggcgtag ttcaaaccag cgccattgag
     1801 cgaattctgg atgaacgtct tctcatcctt gttgattttt tcgtcgagtt ccttgatatc
     1861 ctgttcgagt tcggctttcg tgtcatgctc gataatccga tcctgaatct tcgaggtttc
     1921 ggtgctcttc gagaagaacc agagaaagct cttcttggca tcgatctgtc ccagactgtc
     1981 attcagtttc ttcagattgt cttccgcctg ctgacgcgct tccgtttcct tggttttcag
     2041 atcctggagt tctgttttgc tcataccggc aagcatttcg tcggtcaggt tcttggcgaa
     2101 gaaaccctct ttggcatcaa tcttcgatgc cgtcagctcc gaggattcgg tggcggtcaa
     2161 ctcacgaatc ttcaggtgct tgcctttggt gatcgccgtg tagtattcgt cgcggacgaa
     2221 gaccgtcgtc ttttccgtat gcttctcgat ctttcccgtc atcgtgttct tgcgttcgcc
     2281 atccatggtg atgtcaaaac gcgcgctgcg accgctttct gaatcgttcg ctaccgatgc
     2341 catggcactg cccttcacct gcagcgtcga ttcactgccg cgcgcgacat cgagactgat
     2401 cagattggtg ctcatgatgc gcttaccgtc gtagtcgccg cctgattccg tgcgagacaa
     2461 cacgtgactg acttcgttga actcccaggt gactttcacc gtcttggtcg tcggtttgcc
     2521 atctttgtcg gccacatatc gatcgagcgt gaaggaatag gagttgccgt tctgaacggg
     2581 atcagtgaga gtaccggctt tcattgactt gaaatcgctg cccatgcggt cgagagcgat
     2641 gcgaatgtca tgctgcaggt tctggcgaag gttcgcggtg gtgatgtctc gccgtgccga
     2701 cgaccagatc tgaaagccag cacccaggac gatgctcgat aaaatgaccc ctaccatgat
     2761 ctcaacgagt gttaatccac gtttcataac ggcatctcct ttgtccgatc aatcggaatc
     2821 ggggttgtgc agggcattgc caatgcggag cgttcgaggc tggttcaccg tggtaccgtc
     2881 cccttctcgc cagttcactt cgacctgaat ccagacctcg cgcaatttgt ccttgtcgtc
     2941 cggatgtcga agcggcttca gttgcgcctt atacttgaag tctttcaacg atttgccgaa
     3001 ctccttgaac tccttggccg agacaggata atactcttca gcaatatcgg tcagtgtttt
     3061 atcgggagtc gatttgtact tttccgcata cttcttgatc ggagtgtgtt tacactcctc
     3121 gatcagactc tgagcaagtt tggctgcctt gctgtagttg ttgatcttga ccgtcgagcg
     3181 gttgccgaac tgaatcgcgg acagaatcgg aatgaaagcg acggcaatga tcgctgcggc
     3241 gatcaggatt tcgagcattc ccacgccgcg cgatctgatg acgatgaagg acattggacg
     3301 catggcgatc atttcacctt ccagctttcc cacgtcgggc tcagagttac cagatagcgc
     3361 tccgggtcgt attttccgta atccgggtgc agcgaaaaga gtgacgatcg agtcttgtag
     3421 ccgacgtatt ccaccatgat gtcaccggaa gaggttgcct tgttccagac gttggtgatc
     3481 cagttgccca cgatacgcag acccttgttc ttgggcacgg caatgccttt gtcggtatag
     3541 acgcagcct
//
LOCUS       DAORHD010000398         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29382_length_3545_cov_3.857020, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000398 DAORHD010000000
VERSION     DAORHD010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29382_length_3545_cov_3.857020"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PKO06_09450"
     CDS             <1..494
                     /locus_tag="PKO06_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69911.1"
                     /translation="PPSVVLKILCACLVPRKAGLFLHGALLARDNQGILLTGTSGSGK
                     STLAKLWMQRSSFCGSDEAVIVHKCDGNWHAYPTPFWGAVPPVFTATPHEYKITAHYS
                     LIPPGFPSLMKCVFSGDVHANELSLQHAAAFLGETPPSFLSRQRDIIDRMNILERSLS
                     CCD"
     gene            482..748
                     /locus_tag="PKO06_09455"
     CDS             482..748
                     /locus_tag="PKO06_09455"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HNV69912.1"
                     /translation="MLRLNRANLAWQQFQQRVFLLTVRNNHMHELNAVGSHLFLWLWE
                     HEGITESELVQRVCDSFDVSPEHAGADVERFLNDLQTKEILTRE"
     gene            745..1701
                     /locus_tag="PKO06_09460"
     CDS             745..1701
                     /locus_tag="PKO06_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV69913.1"
                     /translation="MIDFFDGVRQTGGISSFFWEVTARCNQHCPICYLPEPASHDELP
                     ASLISRLIRELRELHCLTVTLSGGEPFLRPDLLDLITMFRESGMGVVVFTNGSCVENR
                     HLIELARLGVSRLEVTVFSTMPEVAAAMGCHDVAHVMQTVTQAMHLGIIVTIKTPVTT
                     RNYPSLPELVTWCQQLGLRHQLDPLIQSPEDKHSSPGDLLEPRQAAQLLGDVTCSCNL
                     SADTIAMRWDGMLIPCLTIPRPIGRWPQQSLREVIESPITRQVWQQLGELPVRCRSCA
                     LVEQCPRCPGLFHSECAGEPWPYLCDIAQAQADERHKAKRLR"
     gene            1825..>3545
                     /locus_tag="PKO06_09465"
     CDS             1825..>3545
                     /locus_tag="PKO06_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69914.1"
                     /translation="MKRSSSLIFCLTLLLVLLHQQSCFASAIARFYGRTLVSSTNATN
                     PGLATGTPDSLYATLANTSNVLVGNFVDASYSEANNGNITAVWIICNKKIDATFNNDQ
                     FNLRYNISGAWGATSLSEVPGNTSFEEANLYVTNDRTWTWANIQNMQVQARYVRVPAY
                     DGRQIHVDTCGIVVRTALRCQGTSLIAANVSQGQPLVPVESVAITSFNGGTALIDGFS
                     ITRQGTAANSDAPRVYVYEDTNQNNAIDGGDLLLGQANVTANPQTVTFASPMSVTAQT
                     KRVIIAYDISTTAVAGRTIGARLANNAAVLITAPDTIQNINFPMTSSLATIRTPPIIA
                     FTLSPSPTSLLTGQAITLTGTVTNSGQTTAVGLTPSTISVNQVSGGNAILSVGPTPAS
                     MNLAAGSSSSFSWTFTAQTSGIINFSGTMTWTDANSQLASTSTLTTSGNVTITTNPGS
                     NSLTTSLTATPQPTVNTNYGPITLKMTVTNVGAGTANNITPSAITMPGLAANLLTGPT
                     PANFATLNPAEVNTFTWTYQSQGTPGTLYFRGNALVNGGPASSTVSNSNFLQVQTPAA
                     LSAVMTAL"
BASE COUNT          743 a         1086 c          928 g          788 t
ORIGIN      
        1 caccgccatc cgtcgttctg aagattctgt gtgcctgcct ggtgccacgc aaggccggac
       61 tgtttctgca cggcgccctg ctggcccgcg acaaccaggg tattctgctg accggcacct
      121 caggcagtgg gaaatcgacg ctggcaaagc tctggatgca gagatcctcc ttttgcggtt
      181 ccgatgaagc ggtcatcgtc cacaaatgcg acgggaactg gcatgcgtat cccactccat
      241 tctggggggc agtgccgccg gttttcacgg caacaccaca cgagtacaag ataactgcgc
      301 actatagtct tattcctcca ggatttcctt cactcatgaa gtgcgttttt tccggagatg
      361 ttcacgccaa cgagctctct ctgcagcacg ccgctgcatt tcttggagag acgccgcctt
      421 catttctctc acgtcagcgt gatatcatcg atcgcatgaa cattctcgag cgttctctgt
      481 catgctgcga ctgaatcgcg ccaatcttgc ctggcaacag tttcaacagc gggtctttct
      541 gttgaccgtg cgcaacaacc acatgcacga attgaacgcg gtgggaagcc acctctttct
      601 atggctgtgg gagcatgagg ggatcactga gtccgaactt gtgcagcgag tgtgcgacag
      661 ctttgacgtg tcgccagagc atgcaggcgc tgatgtcgag cgttttctga acgacctgca
      721 gacaaaggag attctgactc gcgaatgatc gatttctttg acggggtcag gcagacaggc
      781 ggtatctcct cttttttctg ggaggtcacg gctcgctgca atcagcactg tccgatctgt
      841 tatcttcctg aacccgcttc tcatgacgaa ttgcctgctt cgctcatttc acgtctgata
      901 cgtgagttgc gtgaattaca ctgtcttacg gtgactctgt ctgggggtga gccgtttctc
      961 cgccccgacc tcctggatct gatcacgatg ttccgcgaga gcggcatggg cgtcgtggtg
     1021 tttacgaatg gatcctgcgt cgaaaatcgg cacctgatcg agctagcccg actcggcgtt
     1081 tcgcgcctcg aggtgacggt tttttcgacg atgcccgaag tcgctgcggc catgggctgt
     1141 catgatgtcg cacacgtcat gcagacggtc acccaggcca tgcacctggg catcatcgta
     1201 accatcaaaa cccctgtcac gacaaggaat tacccgagcc tgccggagct cgtcacgtgg
     1261 tgccagcagc ttggcctgcg tcaccagctc gacccgctga ttcagtcccc agaagacaaa
     1321 cattcatcgc ccggtgatct gctcgaacca cggcaggctg cgcaactgct gggtgatgtg
     1381 acctgcagct gcaacctcag cgctgacacc atagccatgc gctgggacgg aatgctgatc
     1441 ccctgtctga ctattcctcg tccgatcggt cggtggccgc aacagagtct gcgcgaggtg
     1501 atcgaatcgc cgatcacgag gcaagtctgg cagcagcttg gggaacttcc ggtcagatgt
     1561 cgctcgtgcg cgttggtcga gcaatgcccc cgttgtcccg gactgtttca ctccgagtgc
     1621 gcgggcgaac cctggccgta tctctgcgac atcgctcagg cgcaggccga cgaacgacac
     1681 aaggcaaagc ggctcaggtg attcaaaacg aaggggccag ttgatccgcg gttcctggcg
     1741 caacagtttt cgggtattgt ttttgaaaac ctttttgcgg tacagtttca gaagagattt
     1801 gaccccaccc ggggaggtag tttgttgaaa cgctcgtctt ctctcatttt ttgtctcact
     1861 cttctgctcg ttcttcttca ccagcagtcc tgttttgctt cggccatcgc tcggttttac
     1921 ggccgcacgc tggtctcgag caccaacgcc accaaccctg gtctggcgac aggcactcct
     1981 gactctctct atgccaccct ggccaacacc tcgaatgtcc tggtcggcaa ctttgtcgat
     2041 gccagttatt cagaagcaaa caacggcaac atcacggcgg tctggatcat ctgtaacaag
     2101 aagatcgacg ccacgttcaa caatgatcag ttcaacctgc gctacaacat ctccggagct
     2161 tggggagcaa ccagcttgtc agaggtgccc ggcaacacct cgtttgaaga ggcaaacttg
     2221 tacgtcacca acgaccgcac ctggacctgg gccaacatcc agaacatgca ggtgcaggca
     2281 cgttatgttc gtgttcccgc ttatgacggc agacagattc acgtcgacac ctgtggcatt
     2341 gtcgtccgca cggccttgcg atgtcagggt acaagtctta ttgccgcaaa tgtctcgcag
     2401 ggacaaccgc tcgttccagt cgaatctgtg gcgatcacgt cgttcaatgg tggtaccgcc
     2461 ctgatcgatg gtttctccat cacccgacag ggaacggcgg caaatagtga cgccccccgg
     2521 gtctacgtct acgaagacac gaatcagaac aacgcgatcg atggaggcga tcttctgctt
     2581 ggtcaggcca acgtcacggc caatccgcaa acggtgactt tcgcctcacc catgtcagtt
     2641 acggctcaga caaagagagt catcatcgcc tacgatattt ccacgacggc ggttgccggt
     2701 cgaaccatcg gcgctcgtct tgccaataat gcggcggtac tgatcacggc gcccgatacc
     2761 atccagaaca tcaactttcc catgacctcc agcctcgcga ccatacgcac tccgccgatt
     2821 atcgcgttca cgttgtcacc atcgccgacc agtcttctga caggacaagc gatcacactg
     2881 accggtactg taaccaacag cgggcagacc actgccgtcg ggctcacgcc gtcgacaatc
     2941 tccgtgaatc aggtttccgg aggaaacgcg attctgtccg tcgggccgac ccctgcgtcg
     3001 atgaatcttg cggctggcag ttcaagttct ttttcgtgga cattcacggc gcaaacgagc
     3061 ggtattatta atttttcagg taccatgacc tggacagatg cgaactcgca actcgcttcg
     3121 accagcactc tcacgaccag tggcaacgtg accatcacga ccaaccccgg cagcaacagc
     3181 ctgaccacgt cgctgacggc aaccccacag ccgacggtga ataccaatta cggcccgatc
     3241 actctgaaga tgaccgtcac gaacgttgga gcgggaacgg cgaacaacat cacgccatcg
     3301 gccatcacga tgcccggact cgccgcgaat ctgctgaccg ggccgacacc ggcgaacttt
     3361 gccacactca atccggccga ggtcaacact ttcacctgga cctatcaatc gcagggtact
     3421 cccgggacct tgtattttcg cggcaatgcc cttgtgaacg gaggaccggc cagttcaacc
     3481 gtctcgaact cgaactttct gcaggtgcaa acgccggcag ccttaagcgc cgtcatgact
     3541 gccct
//
LOCUS       DAORHD010000399         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29395_length_3544_cov_4.283462, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000399 DAORHD010000000
VERSION     DAORHD010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29395_length_3544_cov_4.283462"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKO06_09470"
     CDS             <1..329
                     /locus_tag="PKO06_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV69915.1"
                     /translation="DEDLFMPDSTQARLRAPLENTAFVQESVMTMPFKEAKELVLKGF
                     ERKYFENLLEKSRGNISKAARIAGMHRKNLYLKLRELKIRTKDDEDNPEEEDLMPDTE
                     TPNSDR"
     gene            complement(336..1634)
                     /locus_tag="PKO06_09475"
     CDS             complement(336..1634)
                     /locus_tag="PKO06_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNV69916.1"
                     /translation="MNWSFLLLMLAAFLLGVVWHRLVAKPRPHSMANEELQPLFELAL
                     QSSTQGLALLNDQGQVLFWNRRMEGLTGISSLDASQRLLHEIPAFSADHAIVRMISGV
                     TNQPLLHPLVFRTQLTRTSPGQPAPMLVTLSRISPDLPPSSSFLLITLQDISESEELR
                     DRLQMALYKAETSVKKMAELDRLKSEFLAICSHELKTPLVSITGYLDLLQAEKFGPIT
                     AKQRDALDVSIRNASRLNEILSSLLDFARMEAGKMRFEFLAHSLQLMIEEIVKIMEPL
                     ITARQVSLKVELPPDLPHVYMDSGLIHRVFLNLIDNAVKFTPTGGVVTLRAWADEQLV
                     FAEVSDNGRGIDPEKIARVKEPFFQADASNTRTTGGLGLGLAIVEKILAGHGSSLELT
                     SLPGQGTTARFALKKAVRSASGTFLAVPSGMAVPPSLQHR"
     gene            complement(1636..>3544)
                     /locus_tag="PKO06_09480"
     CDS             complement(1636..>3544)
                     /locus_tag="PKO06_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69917.1"
                     /translation="PNFLAAFLMSTALVTCGLLRYDDTASSSTRVALGISFLVQLTTL
                     LLTKCAGALLCFLLGLSMFWWRFWEVPTRRWFRLSPFLAGILLSALLISLYGIFSIAA
                     RHYPWESLSKTPWEHLAFVTRVLEFHMGFSLFLQHPLSGLGPGASPYLLASLRPPLGH
                     LLGLASFNEDPHSLALTLLSETGLFGLFAFCSLIAAGFGIHRWHRYRHTPHLPSGEGI
                     PTPMHQTSPLSTELPVVFAVPAISLLLHALFNNTLSILPLALHLLLLTACHQASCLRE
                     VQWYRHINWMRLPWLPVIPLFLVTGWSLEHNHQVEARLLFAGQQAADNSRYDEAEQAF
                     AAVLNANQQSLRGLFGLAISQERQGRLAQAQDLLGKLDQLSPNVFGVRLHLSRIFLER
                     RQLLEAHRWALLHLQWSQSPAAYELLGRILLLEGRLQEAEQVFQEGLFHVPVQREDEV
                     RAADRMRLLLGELALDRGEMETARHFLQSLTTTEATSPAALFLLGRLNFDAGLTSEAL
                     LFFEKAYKSDPKDARIGNALGYLLSEMNRDLPRARGILEQAYQQYKDQAAPRLQDILS
                     VTHSLGMLYARIGEVAKARELLTLAFDQTPVDWRAAKEARAQDLNRFLASLASPAASP
                     VASSTPAGKEP"
BASE COUNT          832 a          936 c         1117 g          659 t
ORIGIN      
        1 aagacgagga tctgttcatg cctgattcga cacaggcacg gctgcgcgca cccctcgaaa
       61 acacggcatt tgtgcaggaa agcgtgatga ccatgccgtt caaggaggcc aaagaactgg
      121 tcctgaaggg gtttgagcgc aaatacttcg agaatctgct ggaaaagtcc agggggaata
      181 tctcgaaagc cgcgcgaatc gccggaatgc atcgcaagaa tttgtatctg aaactgcgcg
      241 agctcaagat tcgtacgaaa gacgacgaag acaatccgga agaagaggat ctgatgccgg
      301 ataccgaaac accgaactcg gacagataaa gttgatcagc ggtgttgcag cgatggggga
      361 acggccatcc ccgagggaac ggcgagaaag gttcccgagg cggaacgaac cgcctttttg
      421 agggcaaatc gcgctgtcgt gccttgcccg ggtagactgg tcagttcgag gctgcttccg
      481 tgcccagcca gaatcttctc gacgatggcc aaccccagcc caagaccgcc tgtggtgcgg
      541 gtgtttgagg catcggcctg aaaaaatggt tccttgacgc gcgcaatttt ctccgggtcg
      601 atcccgcgcc cgttatctga tacctcggca aacaccagct gttcgtctgc ccaggcgcgc
      661 agggtgacca cgccgcccgt cggggtgaac ttgactgcat tatcaatcag gttgagaaag
      721 acgcgatgaa tcagacccga atccatgtag acatgcggca ggtcaggtgg cagttcgact
      781 ttgaggctga cctgtcgtgc ggtgatcagg ggttccatga tcttcacgat ttcttcgatc
      841 atcagttgca ggctgtgtgc gagaaactcg aaacgcattt tgcctgcttc catgcgcgcg
      901 aaatccagaa gtgaactgag aatttcgttg agtcgcgacg cgttccgaat cgaaacgtcc
      961 agggcatctc tttgtttggc cgtgatcggg ccgaacttct cggcctgcag cagatcgagg
     1021 tagcccgtga tcgaaaccag cggggtcttc agttcgtgac tgcagatggc gagaaactct
     1081 gatttcaacc gatcgagctc agccattttc ttcacgctcg tctcggcctt gtacaaggcc
     1141 atctgcaaac gatcgcgcag ctcctcggac tcgctgatat cttgaagcgt gatcagcaga
     1201 aacgatgacg agggcgggag atcgggactg atccggctca gtgtcaccag catcggagcg
     1261 ggttgcccgg gactggttct ggtcagttgg gtacgaaaga ccagcggatg cagcagaggc
     1321 tggttcgtga cgcccgagat catgcgcacg atggcgtggt cggccgaaaa agcgggaatc
     1381 tcgtgcagga gtctctgtga ggcatccaga gaagagatgc cggtgaggcc ttccattctg
     1441 cggttccaga agagtacctg accctgatca ttcagtaagg cgagaccctg cgtgctgctc
     1501 tgcagtgcga gctcgaacaa aggttgcagt tcttcgtttg ccatactgtg aggtcgcggt
     1561 ttcgcgacca gccggtgcca gacgacaccc agaagaaagg cggcgagcat caggaggaga
     1621 aaggaccagt tcacgctaag gttcctttcc agccggggtt gacgaggcga caggtgaagc
     1681 cgccggggaa gccagcgaag ccagaaaacg gttcaaatcc tgagctcgag cctccttggc
     1741 ggctcgccag tcaacgggag tctgatcaaa agccagagtc agcagttcac gggctttggc
     1801 gacttcaccg attcgggcat acagcatgcc cagactgtgc gtgacgctca gaatgtcctg
     1861 cagtcgaggc gctgcctgat ctttatactg ctgatacgcc tgttccagga taccgcgggc
     1921 gcgcgggaga tcgcggttca tttcactcag aagatatccg agggcgtttc cgatgcgggc
     1981 atccttggga tctgacttgt acgctttttc gaagaacagc agcgcttcgg aggtcaggcc
     2041 ggcatcgaaa ttcagacgcc ccagcagaaa cagggccgcg ggtgacgtcg cttcggtggt
     2101 cgtcaggctc tgcagaaagt gacgggctgt ctccatttcc ccgcgatcga gggcgagttc
     2161 tcccagaagc agacgcatgc ggtcggcggc gcgaacctca tcttcgcgtt gtaccgggac
     2221 atgaaacaac ccttcctgaa aaacctgttc agcttcctga agccggcctt ccaacagaag
     2281 tatgcgcccc agcaactcgt acgctgccgg agactgcgac cactggagat gtaacaacgc
     2341 ccagcgatga gcctcgagca gttgtcgacg ttcgagaaag atgcgagaca ggtgcaggcg
     2401 cacgccgaag acattcggag aaagttggtc cagcttgccc agcagatcct gggcttgtgc
     2461 cagtctgccc tgtcgctcct gagagatggc gagaccgaag agcccgcgca gagactgctg
     2521 attggcgttc agcacggccg caaaggcttg ctctgcctca tcgtatctgc tgttgtcggc
     2581 agcctgctgt ccggcaaaga gcaggcgcgc ttccacctgg tggttgtgtt cgaggctcca
     2641 gccggtgacc agaaagagcg gaatcacggg caaccagggc aggcgcatcc agttgatgtg
     2701 ccggtaccat tgcacttcgc gcagacagga agcttgatgg caggcggtca gcagcagaag
     2761 atggagtgcc aggggcagga tcgacagggt gttgttgaac agggcatgca gcaacagact
     2821 gatggcagga acggcgaaca caaccgggag ttccgtgctc aggggagagg tttgatgcat
     2881 cggcgtcggt atgccttcac ctgagggaag atgaggcgtg tgacgataac gatgccatcg
     2941 atgaataccg aatccggctg cgatcaggga gcagaacgcg aagagtccga agaggccggt
     3001 ctcagagagc agggtcaggg ccagcgagtg cgggtcttca ttgaaacttg caagcccgag
     3061 cagatgtccg agtgggggtc tgagcgatgc cagcagatac ggagaagcgc cgggaccaag
     3121 cccggagagc gggtgctgca ggaacaggct gaagcccatg tgaaactcga ggactcgggt
     3181 gacgaacgcc agatgttccc agggggtctt tgacaggctt tcccaggggt agtgccgcgc
     3241 cgcgatcgag aaaataccgt agagagagat cagcagagcg gacaacagga ttccggccag
     3301 aaaaggcgac agacggaacc agcggcgggt agggacttcc cagaaacgcc accagaacat
     3361 ggacagtccg agaagaaaac acaagagcgc gccagcgcac ttggttaaca gtagggtcgt
     3421 caattgtacc agaaacgaaa tgccgagagc gactctggta gatgaagaag cggtatcgtc
     3481 gtatcgaagc agcccgcagg ttacgagcgc ggtgctcatg agaaacgcgg ccagaaagtt
     3541 cggg
//
LOCUS       DAORHD010000400         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29433_length_3541_cov_3.786575, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000400 DAORHD010000000
VERSION     DAORHD010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29433_length_3541_cov_3.786575"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PKO06_09485"
     CDS             <1..420
                     /locus_tag="PKO06_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69918.1"
                     /translation="GRLYDKAMASFNNALAINPRYAAARYYVGRIFHDQGQFQKAATE
                     YRRVIEDHLGGRIEAPGITVNFGLVFSDLGLLEQAEREYRRLLKEHPDYADLHFHLGM
                     ILKKRNRIDEAMEEFAVAIKLNPQYMDARRKYWDTAR"
     gene            483..2168
                     /locus_tag="PKO06_09490"
     CDS             483..2168
                     /locus_tag="PKO06_09490"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV69919.1"
                     /translation="MFKRKKIGEMLIEAGHITEKELNIGLAEQKKRGKRFGQVLIELG
                     YLTEEKLLPILGKQLKVPFVDLSKVDIKPEVLKMVPEKICRKHMLVPISFDGKTLMVG
                     MADPLDVFIVDEIQFQNNCEVVRAICSETQINDSIDKLFVAGAMAEALGGISADGDKP
                     VDDKAMDALKKAGEETPIIKLVNLILNQAVQKGASDIHIEPDEGELRVRYRIDGTLAV
                     HMTLQPEALASAVSRIKILSGMDISERRIPQDGRIQMIILDKPIDFRVNTIPTAWGEK
                     VCMRILDKSNVQVELENMGFMPENYKRLKKALGCPNGMILVCGPTGSGKTTTLYACLN
                     AIKGVDTNICTVENPVEYKLKLINQVQVRPEIHLDFATVLRAFLRQDPDVIMLGEIRD
                     EETASIAVEAALTGHLVFATLHTNDAASTPPRIIDMGCEPFLVSAAVLCVVSQRLARR
                     VCKFCKDVDPTITPEIIQDLGLPPETINGTFYRGKGCEKCGQSGYKGRVGVHEVLYTS
                     EKIRRLILTKASADEINSCAIEQGMAPMKADIIEKWKQGLTTPEEIFGLVRAE"
     gene            2262..3464
                     /locus_tag="PKO06_09495"
     CDS             2262..3464
                     /locus_tag="PKO06_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV69920.1"
                     /translation="MAKFDCEVKDSKGKVSKVQLEAESLQKLSALLSDKGYFLVKATE
                     VKAAGFSFGFGGSVSQKDLLVFTVQLSTLVGSAIPLVEAVGILADQTENLYFRSVLES
                     VSKDLQSGKTFSSGLRKFPKVFNNIFCNMCEAGEAGGMLDQVLVRLATFAEADAKMRG
                     KIKGALMMPVVQLVMAVGCVIFLLVKIFPNFTKMFKKMKVELPAITSALILASDTLLE
                     HAMACAGAAVGGGVGIYLFIRTDAGQNLISWLSVTLPICGPITKKIAVSRFARTLCSM
                     LAAGVPILQALKITQSVMGNRAMERVLDDMMISVQGGKGLVASIVNNPLFPRLVVKMM
                     EVGENSGNLDKMLLKVADFYDQEVAEALDGLAGALTPILTVVMGIMIGGIALSVFMPL
                     FSIVQKMK"
BASE COUNT          824 a          999 c          931 g          787 t
ORIGIN      
        1 ggtcgactgt atgacaaggc catggcctcc ttcaacaatg cgctggcgat caacccccgc
       61 tatgcggccg cccggtacta tgtggggcgc atctttcacg atcagggaca gtttcagaag
      121 gctgcgaccg agtatcgccg ggtcatcgaa gaccacctgg gcgggcgcat cgaggctccc
      181 ggtatcaccg tcaacttcgg cctcgtgttt tctgatctgg gtttgctcga gcaggccgag
      241 cgtgagtatc gccggttgct gaaagagcac ccggattacg ccgatctgca ctttcatctc
      301 ggcatgatcc tgaaaaaacg caatcgcatc gatgaagcca tggaagaatt cgccgtcgcg
      361 atcaagttga acccgcaata catggacgct cgccgcaaat actgggatac cgctcgctga
      421 cgtttcgccg ccgcggggtt ttcgtgcaac ccaaaacctg atatacttgc cgttcagacg
      481 ccatgttcaa acggaaaaag atcggtgaga tgctcattga agcagggcac atcaccgaaa
      541 aagaactcaa tatcggtctt gccgagcaga aaaagcgcgg aaagcgtttc ggtcaggtgt
      601 tgatcgaact cggatatctt accgaagaaa aacttctgcc cattttgggc aaacagctga
      661 aggtgccgtt tgtcgatctt tccaaggtcg atatcaaacc cgaagttctc aagatggttc
      721 ctgagaagat ctgccgcaag cacatgcttg taccaatcag cttcgatgga aagactctga
      781 tggtgggcat ggcagaccca ctcgatgtgt ttatcgtcga cgagattcag tttcagaaca
      841 actgcgaagt cgtacgcgcc atctgttccg aaacgcagat caacgactcg atcgataaac
      901 tgttcgtggc cggcgcgatg gccgaagctc tgggcggaat ctcagccgac ggcgacaaac
      961 cggttgacga caaggccatg gatgcgttga aaaaggccgg tgaagaaacc ccgatcatca
     1021 agctggtcaa cctgattctc aatcaggcag tgcagaaggg ggcttctgac attcatatcg
     1081 aacccgacga aggcgagctg cgcgttcgtt atcgcatcga tggaacgctg gcagtacata
     1141 tgactctgca accagaagcg cttgcctcgg cggtttcgcg tatcaagatt ctcagcggca
     1201 tggatatctc cgaacgccgt attcctcaag acggccgcat tcagatgatc attctcgaca
     1261 agccaatcga ttttcgcgtc aataccattc cgaccgcatg gggcgagaaa gtctgcatgc
     1321 gcattctcga taaatccaac gtacaggttg aactcgagaa catgggcttc atgcccgaaa
     1381 actacaagcg tctgaagaaa gcactcgggt gtccgaacgg tatgattctg gtctgcgggc
     1441 cgaccggctc agggaaaaca accaccctgt atgcgtgtct caacgccatc aagggtgttg
     1501 acaccaacat ctgtaccgtc gaaaacccgg ttgagtacaa attgaaactc atcaatcagg
     1561 ttcaggtgag acccgagatt cacctcgact ttgctacggt tctacgcgcc tttctccgcc
     1621 aggaccctga cgttatcatg cttggtgaga ttcgcgacga agaaaccgcg agtatcgcgg
     1681 tcgaggccgc gttgacgggt caccttgtct tcgcaacctt gcacaccaac gatgccgcca
     1741 gtactccgcc acgcatcatc gatatgggct gcgagccctt tctggtctca gccgccgtgc
     1801 tctgcgtcgt ctcacagcgt ctggcccggc gcgtctgcaa gttctgcaaa gacgtcgacc
     1861 cgacgatcac ccccgaaatc attcaggatc tcggcctgcc gcccgaaacg atcaatggaa
     1921 cgttctatcg cggcaagggc tgtgaaaaat gcgggcagag cgggtacaag gggcgcgtcg
     1981 gcgtccatga ggtcttgtac accagtgaaa agatccgacg tttgattctg acaaaagctt
     2041 ccgccgatga aatcaactcc tgtgccattg aacaaggcat ggcaccaatg aaagccgaca
     2101 tcatcgagaa gtggaaacag gggctgacga cccccgaaga aattttcggt ctggtgcgcg
     2161 ccgagtgatt tcctctcccg tttttgtctg tggtatgctt ggtcagttca cgcgctcctg
     2221 caggcaccca cgcctcatga atctcagcga ttaggactgt tatggccaag tttgattgcg
     2281 aagttaaaga ctcaaaaggc aaagtcagca aggtacaact cgaagcagaa tcgctgcaga
     2341 agctgtcagc gctgctcagc gacaaggggt atttcctcgt caaggccacc gaggtcaagg
     2401 ctgccggctt cagcttcggc tttggcggca gcgtttcaca aaaagatctg ctcgtcttta
     2461 cggttcagct ctcaactctt gtcggttcag ccattccgct cgtcgaagct gtgggcattc
     2521 tcgcagatca aactgaaaac ctgtacttcc gatccgttct cgaatccgtc agcaaagacc
     2581 tccaatccgg taaaacattc tcttcgggat tacgcaagtt tccgaaggtt ttcaataaca
     2641 tcttctgcaa catgtgcgaa gccggcgagg ctggcggtat gctcgatcaa gtcctggtgc
     2701 gtctggccac cttcgccgaa gccgatgcca agatgcgcgg caagatcaaa ggcgcgctca
     2761 tgatgccggt cgtccagctc gtcatggcgg tcggttgcgt catattcctg ctggtcaaga
     2821 tctttccgaa cttcaccaag atgttcaaga agatgaaagt cgagctgccc gcgattacca
     2881 gcgctctgat tctcgcctca gacacacttc tcgagcacgc gatggcctgt gccggagctg
     2941 ctgtcggcgg aggcgtgggc atttacctgt tcattcgcac cgacgctggt caaaacctca
     3001 tttcctggtt gtcggtcact ctgccgatct gcggcccgat cacgaaaaaa atcgcggtat
     3061 cacgatttgc ccgtaccttg tgttcgatgc tcgctgccgg cgttccgatt ctgcaagcac
     3121 tgaagattac acagtcggtc atgggcaacc gcgctatgga acgggtgctc gacgacatga
     3181 tgatcagcgt ccaaggcggt aagggcttgg tcgcctctat cgtgaacaat cccttgttcc
     3241 cccgcctggt cgtcaagatg atggaagtcg gagaaaactc cggaaacctc gataagatgc
     3301 tgctcaaagt cgccgacttc tacgaccagg aagtcgccga agcgctcgac ggccttgccg
     3361 gcgctctgac cccgattctc accgtcgtca tgggtatcat gatcggcggt atcgctctct
     3421 cggtctttat gccgctcttc tcgatcgtcc agaagatgaa gtagtcagca tcgtcttcag
     3481 gtcacacgtc gactcagcta tccacgacgc tttctcaaca cacattatcg cgctcaggtc
     3541 g
//
LOCUS       DAORHD010000401         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29435_length_3541_cov_3.445496, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000401 DAORHD010000000
VERSION     DAORHD010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29435_length_3541_cov_3.445496"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKO06_09500"
     CDS             complement(<1..358)
                     /locus_tag="PKO06_09500"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNV69921.1"
                     /translation="MASKKQQVAVVAVGGNSLIQDEKRVTVADQYQAAWNTMVHVCDM
                     LEQGFNVVVTHGNGPQVGYVLMRSEIAAKQLHEVPLDSCVADTQGAIGYNFQMALANE
                     FKKRGMKRNAVTVVTQV"
     gene            complement(406..1617)
                     /locus_tag="PKO06_09505"
     CDS             complement(406..1617)
                     /locus_tag="PKO06_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNV69922.1"
                     /translation="MIDQALVKKIQAEVKKHEPAIRKFMRDLCRIPSFDSKIGDVVKR
                     INEEMKMLGFDEVFVDKMGNSVGRVGNGPTKIVFDSHVDTVGIADRTQWKWDPFEGKI
                     ENDILYALGAVDEKGSTPGMVYGLKILKDLNLLDGYSLYYFGNMEEECDGIAPNSLVD
                     TEKIKPDFVIVGEPTCMRIYRGHRGRVELKVTTKGRTCHASAPERGENAVYKMVPIIK
                     EISEMSHMFKNDAFLGKGSVVVSKIECKTPSLNAVPDECTIYLDRRLTFGETEESAIK
                     ELESLPSMRNGKVEELFYDVPSYTGFVFPVSKYYPPWALDPKHPLVQAGVDAYKTVHD
                     GAEPTVDKWVFSTNGTYWMGKANIPSIGFGPGNEVYAHTVNDQVPLAEVVKCTEFYAA
                     LPLALKNFLKK"
     gene            complement(1664..3001)
                     /gene="ssnA"
                     /locus_tag="PKO06_09510"
     CDS             complement(1664..3001)
                     /gene="ssnA"
                     /locus_tag="PKO06_09510"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HNV69923.1"
                     /translation="MKIIGHGTILTFGSDHRVIQDGAVAYDDERIHAVGTTEEIKKRF
                     SKAKVRNVHGRVITPGLVNAHMHLYSTFARGMALKDEAPENFVQILERLWWRLDRALT
                     LEDLELTAMIPLLQAVRSGVTTLVDHHASPHALAGSLDILKKAFRRVGVRGCLCYEVS
                     DRDGASRRDAGLEENANFIRGIKLGDTNDIAGMIGLHASFTLEDTTLARAAELAKALK
                     VGVHIHVAEDKSDVEDAKRRGFRSPVDRLHRAGLTGPDSIFAHCVHADSLDIQTLALT
                     RTNVIHNPRSNMNNAVGCAKIEDMIKERVPVAFGTDGMSPSPLPDLYTASLIHKHQAG
                     DPRKGWSYLWKMFSQNTPAVASTVLRTPLGLLEAGAGSDIVVWDYIPPTPISDGNTLG
                     HFLFGLSEARVNTTICQGKTLFEDNKLTFLDDSEEIELHAKARELAQALWERF"
     gene            complement(3082..>3541)
                     /locus_tag="PKO06_09515"
     CDS             complement(3082..>3541)
                     /locus_tag="PKO06_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69924.1"
                     /translation="LICESGFTPDESIDPGTWVGFLGELAEVPSGPASTSVPVWRVSH
                     DSGISAKDPFSRHPSYHLFQGVFTKAGAGVFSLAAPRDTDVRLSVLWPEVATFKNLDE
                     VFGVNLARPICLRGTIASWSADHTLPTCVRADRIRKDADDFEEDYTNEDR"
BASE COUNT          663 a          963 c         1115 g          800 t
ORIGIN      
        1 gcacctgggt gaccacggtg acggcgttgc gcttcatgcc gcgcttcttg aattcgttgg
       61 ccaaagccat ctgaaagttg tacccgatcg cgccctgggt atcagcgaca cagctgtcga
      121 gcggaacttc gtgcagctgc ttggcggcga tctcagaccg catcagcaca tacccgacct
      181 gcggcccgtt gccatgcgtg accacgacgt tgaagccctg ttcgagcatg tcgcagacat
      241 ggaccatggt gttccacgcg gcctgatact ggtcggccac ggtgacgcgc ttttcatcct
      301 ggatcagaga gttgccgccg acggcgacga cggcgacctg ctgtttcttt gatgccatat
      361 atgtacgagt tagcctttcc tgagatattg atgaattcta gtgaattact tcttgagaaa
      421 gttcttgagc gccaggggca gggccgcgta gaactcggtg catttcacga cttcggcgag
      481 cggaacctgg tcgttgacgg tgtgggcgta gacttcgttg cccgggccga acccgatgct
      541 cggaatgttg gctttgccca tccagtaggt gccgttggtc gagaagaccc atttgtcgac
      601 ggtcggttcg gctccgtcgt gaaccgtctt gtaggcatcg acgccggcct gcaccagcgg
      661 gtgcttcgga tcgagcgccc agggcggata atacttgctg accggaaaca cgaagccggt
      721 gtagctgggc acgtcgtaga acagttcctc gaccttgccg ttgcgcatgc tgggcagcga
      781 ttcgagttcc ttgatcgcgc tttcttcggt ttcgccgaag gtgaggcggc gatcgagata
      841 gatggtgcac tcgtcaggaa ccgcgttcag actgggagtc ttgcattcga tcttggagac
      901 cacgacggac cccttgccca ggaacgcatc gttcttgaac atgtggctca tctcgctgat
      961 ttccttgatg atcggaacca tcttgtacac agcgttctcg ccgcgctcgg gggcggaagc
     1021 atggcaggtg cggcccttgg tggtgacctt gagctcgacg cggccgcgat gtccgcgata
     1081 gatgcgcatg caggtcggtt cgccgacgat gacgaagtcg ggcttgatct tctcggtgtc
     1141 gaccagcgaa tttggcgcga tgccgtcgca ttcctcttcc atgttgccga aatagtagag
     1201 cgaatacccg tcgagcaggt tcaggtcttt caggatcttg aggccgtaga ccatgccggg
     1261 tgtcgagccc ttttcatcga cggcgccgag cgcgtagaga atgtcgttct cgatcttgcc
     1321 ttcgaatggg tcccacttcc attgcgtgcg atcagcgatg ccgacggtgt cgacgtggct
     1381 gtcgaacaca atcttggtgg ggccgttgcc gacgcgaccg acggagttgc ccatcttgtc
     1441 gacgaagact tcgtcgaacc cgagcatctt catctcttca ttgatgcgtt tgacgacatc
     1501 gccgattttc gagtcaaagc tcggaatgcg gcacagatcg cgcatgaact tgcgaatcgc
     1561 gggctcgtgc tttttcactt cggcctgaat cttcttcacc aatgcttgat cgatcattcc
     1621 tgtattcgct ccttgttgtg atgaaaccgc ggtgacgtgt tgattaaaag cgttcccaga
     1681 gggcctgcgc cagttcgcgg gccttggcgt gaagctcaat ttcttcgctg tcgtcgagga
     1741 aggtcagttt gttgtcttcg aacagggtct tgccctggca gatcgttgtg ttaacgcggg
     1801 cttcgctgag gccgaacagg aagtggccga gtgtgttgcc gtccgagatc ggggtcggcg
     1861 gaatgtaatc ccacaccacg atgtcgctgc cggcaccggc ttcgagcaga cccagcggag
     1921 tgcgcagcac ggtcgaagcc acggccgggg tgttctgcga gaacatcttc cagaggtagc
     1981 tccagccttt gcgcggatcg ccggcctggt gtttgtgaat caggctggcg gtgtacaagt
     2041 cgggcagcgg gctcggcgac attccatcgg tgccgaaggc gaccggaacc cgctctttga
     2101 tcatgtcttc gatcttggcg caacccacgg cgttgttcat gttcgagcgc ggattgtgaa
     2161 tgacgttggt gcgggtgagc gccagcgtct gaatgtcgag cgagtctgcg tggacgcagt
     2221 gggcgaagat actgtcgggg ccggtgaggc cggcgcgatg caggcgatcg accgggctgc
     2281 ggaagccgcg gcgcttggca tcttcgacat cggacttgtc ttcagccacg tgaatatgca
     2341 cgccgacctt gagggccttg gcgagttcgg cggcgcgggc cagggtggtg tcttcgaggg
     2401 tgaaagaggc atgcaggccg atcatgccag cgatatcgtt ggtgtcgccg agcttgatgc
     2461 cgcgaatgaa gttcgcgttc tcttcgaggc cggcgtcgcg ccgtgacgca ccgtcgcggt
     2521 cagagacttc gtagcagagg cacccgcgca cgccgacgcg gcgaaacgcc ttcttgagaa
     2581 tgtcgagcga accggccagc gcgtgggggc tggcgtggtg gtcgacgagc gtggtgacgc
     2641 ccgagcgaac cgcctgcagc agcggaatca tcgcggtgag ttcgagatct tcgagggtca
     2701 gagcccggtc gagacgccac cagagacgtt cgagaatctg gacaaaattc tcgggggctt
     2761 catctttgag agccatgccg cgggcaaaag tggaatacag gtgcatgtgc gcattgacaa
     2821 gcccaggggt gatgacccgg ccgtgaacgt tgcgaacctt ggctttggaa aagcgtttct
     2881 tgatttcttc cgtcgtgccg accgcgtgga tgcgctcgtc gtcgtaggcg accgctccgt
     2941 cctggatgac ccgatggtcg gaaccgaagg taaggatcgt accgtgtccg atgattttca
     3001 tctcgctctc cctgagtatc tgtatagaat cgtgctgctt gggcgaaatg tatcacaggg
     3061 gaggtgagca catcaagaat ttcaccggtc ttcgttcgtg taatcctctt cgaaatcatc
     3121 cgcatctttt ctgattctgt ctgctcttac gcaggttggc agcgtatgat cagcggacca
     3181 gctggcgatt gtgcccctga gacagattgg ccgcgcgaga ttgacgccga agacctcatc
     3241 gaggtttttg aaagttgcga cttcgggcca gagaacactc aggcgcacat ctgtatcgcg
     3301 tggggcagca agcgaaaaga ctccggcacc ggcttttgta aacactccct gaaagaggtg
     3361 atacgatgga tgtcgcgaaa atggatcttt ggcagatatg ccggagtcat gtgagacccg
     3421 ccacacagga acggatgttg aggccggacc ggacggaact tcggcgagtt ctcccaagaa
     3481 tccgacccag gttccggggt caatgctctc atcaggagtg aacccgcttt cacagatcag
     3541 a
//
LOCUS       DAORHD010000402         3531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29544_length_3531_cov_3.773590, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000402 DAORHD010000000
VERSION     DAORHD010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29544_length_3531_cov_3.773590"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1106
                     /locus_tag="PKO06_09520"
     CDS             63..1106
                     /locus_tag="PKO06_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV69925.1"
                     /translation="MKMETLLQPEELKPMMEMAGKVLAELDRILLGRSDLHRLVLVGI
                     LARGHLLLEGQPGVGKTALIKALGQLLQLDFKRVQFTPDLMPSDILGAHILQENDQGK
                     REIVFQPGPIFTNILLADEINRASPKTQSALLEAMQERSVTLMGTTRKLPHPFFVLAS
                     QNPIELEGTYPLPEAQLDRFLFKLNVENARVDILEEIIATRRRGEPPAPTWSVRAEDL
                     QRLFLVIDRIFLPRPVARYIARLTAATHAGNAEAPELVKKYITWGASPRAAIAMAEAA
                     RGVALLQGRPTVGFEDVKAVAPSVLNHRLILNYQARFDRVSPQDVIQNLLASLNEAGL
                     DLPADIKVQTANA"
     gene            1099..2853
                     /locus_tag="PKO06_09525"
     CDS             1099..2853
                     /locus_tag="PKO06_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69926.1"
                     /translation="MRNFSLLLCVLVGLLVPLTAFAVEYGNIAVSVESPVGTEFTHGY
                     AEFRVSITNRSLSTAHDVKLTVPGNNYSRETLTVTRTVNVAANSTTQVSLFQPCVAYM
                     NDGMAVEIDGARQSEVVRANCLNQRSYYGPFISVLISKSFQEDDFTLEAKGTSSYNRT
                     FNLHRAESPVAEWSANWLGYSRFDGILLTRDEFDTAPVPVRTALWRYVEAGGTLTVTG
                     GAIDVPAEWRPYSRKDGAWEVTHVGFGKCFMTGSRDAPGLISETERTILDSWNDSNYP
                     FEQRKDIESANNVFKVVEGVEIPVRGLFLLMIGFAVLIGPVNLVVLSNWKKRIWILWT
                     VPLFSFLTCIAVFLYSAFAEGWNAKARFEGVTILDETQHRAVTLGWAAYYATLTPGGG
                     AHYNVDSDLMPQWGRRGGYRSGVERAVNMDWSRDQHLVFGWVVSRVPSHFAIRKNETR
                     RERLTVSASDHAGMTVVNGLGAPIRSLYVAREDGIVFSGSDIPAGKEARLQPTSFTVG
                     ATARQMDYFRGSFTTDWLGLPGRLTTDRLNFLRHNTYLAELDGCPFIEKGLDDIETVK
                     AESVVFGIMKRDGEEAKP"
     gene            2850..>3531
                     /locus_tag="PKO06_09530"
     CDS             2850..>3531
                     /locus_tag="PKO06_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69927.1"
                     /translation="MKIEVVRLRKHFVNAKAVDDISFTFNSGEIIGFVGPNGAGKTTT
                     MRMMATLDEPTDGDVFFDDVSVVESPERVRELMGYVPESLPGHREMTVHEYLDFFARA
                     YGVRGEQRRKIVEEIEEFTNLLGIRDKFLNDLSKGMGQRVCLARALVHDPQVLIMDEP
                     ASGLDPRARVELRELLKVLATQGKAILISSHILTELAEICTGTVIIERGKLLKAGSLQ
                     EITKEECSH"
BASE COUNT          696 a         1024 c         1054 g          757 t
ORIGIN      
        1 gatgggcttt ttccgggtgc ggcatgctat actcgcactc gaaactttct gaaccggggg
       61 agatgaagat ggagaccctg cttcaacctg aagaactgaa gcccatgatg gagatggccg
      121 gcaaggtcct ggcagagctt gaccgcattc tgcttggccg cagcgatctg catcggttag
      181 tactcgtcgg tattctggcg cgtggccatc tgttgcttga aggccagccg ggagttggaa
      241 aaacggcgtt gatcaaagcg cttggccaac tgctgcagct cgacttcaaa cgcgtgcagt
      301 ttacccccga cctcatgccc agcgatattc tcggcgcgca cattctgcag gaaaacgacc
      361 aggggaaacg cgagatcgtc ttccagcccg ggccgatttt tacgaacatt ctgctggccg
      421 acgaaatcaa tcgcgcgtct cccaagacgc agtcggcgct tctcgaagcc atgcaggaac
      481 gatcggtgac cctcatggga accacgcgca aactgcctca tccgttcttc gtgctggcct
      541 cgcagaaccc catcgaactg gaaggtacgt atccgctccc cgaggcccag ctcgaccgct
      601 tcctgttcaa actgaatgtc gagaacgcgc gggtcgatat ccttgaggaa atcattgcca
      661 cccgacgtcg cggtgaaccg ccggctccga cctggagcgt gcgcgccgaa gacctgcagc
      721 gcctgtttct cgtgatcgac cgtatcttcc tgcctcgccc ggtggctcgc tacatcgccc
      781 ggctcacggc ggccacgcac gccggtaacg ccgaggcccc ggagctggtg aagaaataca
      841 tcacctgggg agcctctccc cgtgcggcga ttgccatggc cgaagcggcg cgcggtgtcg
      901 ctttgctcca ggggcggccg acggtcggtt ttgaggatgt caaagccgtt gcgccttcgg
      961 ttctgaatca tcgcctgatt ctcaactatc aggctcgatt tgatcgcgtg tcacctcagg
     1021 atgtgattca gaatctgctg gcgtcgctga acgaggcagg tcttgatttg cccgctgata
     1081 tcaaggtgca gacggccaat gcgtaacttc tctctgctgc tgtgcgtttt ggtgggtctg
     1141 ctggtaccac tgacggcatt cgcggtcgaa tacggcaaca ttgcggtcag cgttgaaagt
     1201 ccggtcggta cggagttcac tcacggttac gcggagtttc gggtcagcat caccaatcgg
     1261 tcgctgtcga cagcgcatga cgtgaagcta accgtgccgg gcaacaatta cagccgcgaa
     1321 acacttacgg tgactcgtac ggtcaacgtc gccgcgaatt cgaccaccca ggtcagtctc
     1381 tttcaaccct gtgttgccta catgaacgat ggcatggctg tcgaaatcga cggtgcgcgt
     1441 cagtccgagg tggtgcgagc caactgtttg aatcagcgca gttattacgg cccattcatt
     1501 tccgttctca tcagtaaaag tttccaggaa gatgatttca cgcttgaagc caagggaacc
     1561 tcttcgtaca atcgcacgtt caatcttcat cgcgccgaat cgccggtcgc cgaatggagc
     1621 gcgaactggc tcggttattc ccgttttgac ggcattctgc tgacccgcga cgaatttgat
     1681 accgcgccgg ttccggtgcg taccgcgctc tggcgctatg tcgaggccgg tggcacgctg
     1741 acggtgacgg ggggcgccat tgatgtgccc gccgagtggc gtccctattc ccgcaaagat
     1801 ggtgcctggg aagtcacgca tgtgggcttc ggcaagtgct tcatgaccgg ttcgcgtgat
     1861 gcgcctgggc tgatcagtga gaccgaacgt accatcctcg attcctggaa cgactcgaat
     1921 tatccgtttg aacaacgcaa ggatattgag tcggccaaca atgtgttcaa agtggtcgag
     1981 ggggtcgaga ttccggtgcg cggcctgttc ctgctgatga tcggcttcgc cgttctgatt
     2041 ggcccggtga atctggtcgt gctctcgaac tggaaaaaac gcatctggat cctctggacc
     2101 gtgccactct tctcgttcct gacctgcatc gccgtctttc tctattcggc cttcgccgaa
     2161 ggctggaatg ccaaggcccg attcgagggc gtgaccattc tcgacgagac acagcatcgt
     2221 gccgtgacgc tcgggtgggc cgcctactat gcgactctga cgcccggcgg cggcgctcac
     2281 tacaacgtcg attccgacct gatgccgcag tggggccggc ggggcgggta tcgcagcggt
     2341 gtcgaacgcg cggtcaacat ggattggagc cgtgaccagc atctcgtttt cggctgggtc
     2401 gtctcgcgcg tgccatcgca cttcgcgatt cgcaagaacg aaacccgtcg cgaacgcctg
     2461 acggtcagcg cttccgatca tgccggcatg acggtggtca acgggttggg cgcaccgatt
     2521 cgcagtctct atgtcgcccg cgaagacggc atcgtcttca gcggctccga tattcccgcc
     2581 ggcaaggaag cgcgactgca gccaacgtcg ttcacggtcg gggccacggc tcggcagatg
     2641 gactactttc gcggcagctt caccaccgac tggctggggt tgcccggccg tctgaccacc
     2701 gaccgactca acttcctgcg tcacaacacg tatctcgcgg aactcgatgg gtgcccgttt
     2761 atcgagaaag ggctcgacga cattgagacc gtgaaggctg aatcggtggt attcggcatc
     2821 atgaagcgag acggagaaga ggcaaagcca tgaagataga agtcgtacgt ctgcgaaagc
     2881 actttgtgaa cgcgaaagcg gtcgatgata tctcgtttac attcaattcc ggagaaatca
     2941 tcggcttcgt cggcccgaac ggcgccggca agaccacgac gatgcgcatg atggcgacgt
     3001 tggatgagcc gaccgatggc gatgtcttct ttgacgatgt ctccgtggtg gagtcacccg
     3061 agcgggtgcg cgaactgatg gggtatgtcc ccgaatctct gcccggccat cgtgaaatga
     3121 ccgttcatga gtatctcgac ttctttgccc gggcctacgg agtgcgcgga gagcagcgtc
     3181 ggaagatcgt cgaagaaatc gaggagttca ccaacctgct tggaattcgc gacaagtttc
     3241 tcaacgacct gtcgaagggc atgggccagc gtgtctgtct ggcgcgcgcc ttggtccatg
     3301 atccgcaggt tctgatcatg gacgaaccag cttcgggcct tgatccgcgc gcgcgggtcg
     3361 aactgcgtga actgctcaag gtgctggcga cgcagggaaa agcgattctc atcagttcgc
     3421 acattctgac cgaactggcg gaaatttgta ccggtacggt gattatcgaa cggggcaagc
     3481 tcctcaaggc gggttcgctg caggaaatca ccaaagagga atgttcccat c
//
LOCUS       DAORHD010000403         3527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29591_length_3527_cov_2.961406, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000403 DAORHD010000000
VERSION     DAORHD010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29591_length_3527_cov_2.961406"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..492
                     /locus_tag="PKO06_09535"
     CDS             139..492
                     /locus_tag="PKO06_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV69928.1"
                     /translation="MVDKFRLTSRREGDVVIVSVFGYIEGSGGSELKQFVDKALEEGV
                     RHFIIDFRGAELISSPGVAELLDVSNHAVDDFDSRMVCYGLDQHHNAVLEMSGFFFLV
                     QQAADEASALQAVHE"
     gene            543..3401
                     /locus_tag="PKO06_09540"
     CDS             543..3401
                     /locus_tag="PKO06_09540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69929.1"
                     /translation="MKAEHDQDSRRTWRLLLRLTLFLMVPLCVCGYLGFFAVDLEESY
                     RRQRARRQLERDLDDWVQRIDPEGTVRLRFQQFAAQLDEDQVSERRIEWLMTVARQKW
                     GLNFEPYVFDADGKLLAPRNLNLRNRPVFQRLWSALEGDPLAIEEADRYRRPLQALLG
                     YEFRLRTILGLSGVLHPIRSQGRDGYLFWTKLRRSGAAGVILVCWSRPSHLELLQHSL
                     QQSTFHRYAILMEERDGRQQVLGRPFPGNREELLKQSGVFGHPWYLSQGWQWASSMIG
                     DSRLVIGVPAPDLTMTQVRQRLWIMLLLIGGCGFWLFHGWFFRGRDLYLSLRVKTLLF
                     FSLAVFAPLMGILFLAFQTVNLRRQILEDRIEREAREQLLALDASFPGSIETYRRFCL
                     ETRQRWITNFDRDAMQKELQKLISERQLVHAEARDLNGDMLLKQMDNRFFEGMESTMN
                     AIAKMCIERNLAQKRKSLALSVDMLDTKTREVLQNRQTGFPKLIAPGEVQNIRFGATE
                     FLYFWDTVDSSQVPIAYILLVQSLAQHAPGYLHELLLKNRKYESDMLRIFARSEISGQ
                     WYPADEAMGGRLDQFMERLRISGRALCDTVFWQGKRYYAVGFPGRNFEDVGLLALSPE
                     RLVTDELRQWHARILAGVVAVLIVAFFTAQLLWREFLTPMRGLSTAMAALRAHSSDTR
                     VEIRTGDELEKLGHAFNRLIGDLKEMELARVVQTALIPQRIPEIPGYEVAIESAIARN
                     LGGDYCDVLPMRDGRFLLVMGDVSGHGVSSALLMAMAKAVAFDYADSGTEPAELLRQM
                     NRLVFQLMNRRKLMTYFAGMLEPGTGHFQFSNAGHPFPLKIMANGLVEELALVHLPLG
                     LLERREAFQVQETVIPPGAFLLLYTDGLVEMTDETGQQYGYERLAEAVAVMKGRAAGV
                     VVESLRELYLRYHGRRELDDDVTFLALARSEPTESCMR"
BASE COUNT          721 a          900 c         1100 g          806 t
ORIGIN      
        1 ccccgcagcc ctgcatgaaa cgggtgcggg caagggacgg acttgtggga gaatccgtgc
       61 aggaaactgg caatcgcttc ccctgacgtg tagaatacag tatacgcact ttcatcttac
      121 caaaacagga agtgaaccgt ggtagataaa tttcgtctga cgtcgcgtcg cgaaggtgat
      181 gtggtcatcg tctccgtgtt tggatacatt gaaggctccg gcggcagcga gttgaagcag
      241 ttcgtcgaca aggctctcga agagggagtt cgacacttca tcatcgattt tcgcggtgcg
      301 gaactgatca gcagtcccgg cgttgccgaa ttgctcgatg tttcgaacca tgccgtggac
      361 gatttcgatt cgcgcatggt ctgctacgga ttggaccaac accacaacgc ggttctggag
      421 atgtccggtt tctttttcct cgtacagcag gcggctgacg aggcgagtgc tctccaggcc
      481 gtgcacgagt aacgagcggt aactcctgat ttcttgctca tgaaacggtg cttgcagcgc
      541 cgatgaaggc tgaacacgac caggactctc gacggacgtg gcggttgctg ctgcgactga
      601 cgctctttct gatggtgccc ctgtgcgtgt gcgggtatct cggatttttt gccgtcgatc
      661 tcgaagagtc ttatcgccgt cagagagcga gacgacaact cgaacgcgat ctcgatgact
      721 gggtgcagcg aatcgaccct gaaggaaccg tgcggctgcg ctttcagcag tttgccgctc
      781 aactcgatga agatcaggtt tccgagcgtc gcatcgagtg gctgatgacc gtcgcgcgcc
      841 agaaatgggg attgaacttc gaaccgtacg tgttcgatgc tgacggaaag ctgctggcac
      901 cgcgcaacct caacctgcgc aatcgaccgg tgtttcaaag actgtggtcc gcgttggagg
      961 gagatccgct cgccatcgaa gaagcagacc gatatcgccg acctctccag gcgctgctcg
     1021 gatatgagtt tcggttgcgg accattctgg gattgtccgg agtgctgcat ccgattcgtt
     1081 cgcaggggcg tgacggctat ctcttctgga ccaaactgcg gcggtcaggc gcggccggag
     1141 tcattctggt ttgctggtcg cgaccgagcc atcttgaact tctgcaacat tctctgcagc
     1201 aaagcacgtt tcatcgctat gccattctga tggaggaacg ggacgggcga cagcaggtgc
     1261 tgggccggcc gtttccaggg aaccgtgagg aactgctgaa gcaaagcgga gtcttcggcc
     1321 acccttggta tctttcgcag ggctggcaat gggcttcatc gatgattggt gacagtcggc
     1381 tggtgatcgg cgtgccggca cctgacctca ccatgacgca ggtccgtcag cgcttgtgga
     1441 tcatgctgct gctgatcggt ggctgtggat tctggttgtt tcacggttgg ttctttcgcg
     1501 gtcgcgatct gtatctgtca ctccgcgtga aaacgcttct gttcttctct ctcgcggtgt
     1561 tcgcgcctct gatgggcatt ctgtttctgg cgtttcagac ggtgaacctg cgccgccaga
     1621 ttctggaaga tcgaatcgaa cgtgaggcgc gtgaacagtt gttggctctc gatgcgtcgt
     1681 tcccggggtc gatcgaaacc tatcgtcgat tctgcctcga aacacggcaa cgatggatca
     1741 cgaacttcga tcgcgacgcg atgcagaagg agctgcaaaa actgatctcc gaacgccaac
     1801 tggtgcacgc ggaagccagg gatctcaatg gcgacatgct gttgaagcag atggacaacc
     1861 ggtttttcga aggcatggaa tcgacgatga atgccattgc caagatgtgt atcgaacgca
     1921 atctggcgca aaagcgcaag agtctggccc tgtcggtcga catgctcgat acgaaaacgc
     1981 gtgaggtgct gcagaatcga caaaccggtt ttccgaaact gatcgccccc ggtgaagtac
     2041 agaatattcg ctttggcgcg accgagtttc tctatttctg ggacacggta gactcttcgc
     2101 aagtgccgat tgcctatata ttgttggtac aatcgctggc gcaacatgcg ccgggatatt
     2161 tgcacgaact gctgctgaaa aatcgtaagt acgagtcaga tatgctgaga atattcgcca
     2221 ggagcgagat aagtgggcag tggtatcctg ccgacgaggc gatgggagga cgtctcgacc
     2281 agttcatgga acgattgcgc atcagcggtc gtgctttgtg cgacacggtt ttctggcagg
     2341 gaaagcgcta ctatgcggtc gggtttcccg ggcgtaactt tgaggatgtc ggactgttag
     2401 cgctctcccc ggagcgtctg gtgaccgacg agttacgcca atggcatgcg cgaattcttg
     2461 ccggcgtggt tgcggtcctg atcgtcgcat ttttcacggc gcagctgctc tggcgggagt
     2521 tcctgacccc gatgcggggg ctgtcgaccg ccatggccgc gctgcgcgca cactcttctg
     2581 atacgcgtgt cgagattcgc accggagatg aactcgagaa gctgggtcat gcgtttaatc
     2641 gcctgatcgg tgacctcaag gaaatggaac tggctcgagt ggtgcagacc gcgctcattc
     2701 cacagcgcat tcctgagatt ccgggatacg aagtggccat tgaaagcgct attgcacgca
     2761 atctgggggg agactactgc gatgtgctgc ccatgcgaga cgggcggttt ctgctggtca
     2821 tgggagacgt ttccgggcat ggagtcagct cggctctgct gatggccatg gccaaggctg
     2881 tggctttcga ctatgccgat tccggtacgg aacccgcaga actgctgcgg cagatgaacc
     2941 gtctcgtgtt tcagctgatg aatcgccgca aactgatgac gtattttgcc ggaatgctgg
     3001 aacccgggac gggacatttt cagttttcga atgccggaca tccgtttccg ctcaagatca
     3061 tggcgaacgg tctggtggaa gaactggctc tggtgcatct gccgctcggt ctgctggaac
     3121 ggcgcgaagc gtttcaggtg caggaaacgg tcattccgcc tggcgccttc ctcctgttgt
     3181 atacggatgg cctcgtcgaa atgacggatg agacgggaca gcagtatggc tacgaacggc
     3241 tggcggaggc ggtggcggtt atgaagggga gagccgctgg agttgtggtc gaaagtttgc
     3301 gagaactgta tcttcgctat cacgggcgac gtgagctgga tgacgacgtg acttttctgg
     3361 cgcttgcccg aagtgagccc acggaaagtt gcatgcgtta gcggggaacg atacaatagg
     3421 gcagggaatg tttggcgcga gtaccggcga atctgggctc tgaaagatcc gaggaaaaga
     3481 ggacgattcg atggccgacg aaaagaaagg tgccttgaag accgtac
//
LOCUS       DAORHD010000404         3522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29646_length_3522_cov_3.690222, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000404 DAORHD010000000
VERSION     DAORHD010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29646_length_3522_cov_3.690222"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /locus_tag="PKO06_09545"
     CDS             complement(<1..2098)
                     /locus_tag="PKO06_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV69930.1"
                     /translation="MARWLVIIIPLFTFGYLWESQVQKRMTETRGRFVRHLQEHVEQM
                     AVAADPELFLQRPMTAALRRFQRNQRVSLSQLSRSLNSVTHGCQFDLFLLHDAGSRLE
                     ASDQAENLWLMRRLLLSLRDASMTREKSREINRLLSNAFGLGIGLERFQRGRGRLIAV
                     PFQGGESYLAWEQTRDAALLIFCRHLPQPEQRFRAALAQVKLSISDWVGMLQPGAEWL
                     ISAKTDRAELEQAYRAFLQTKRSEQEWKSRGFWFVQTRSGETLVVSRPLPSAEGASQT
                     WAVRLAVVLLIPMLGWAGSLFQRRARVRGVVGGLFLVTALVPLLELAQATMMSFEVRR
                     SLLKTQVERGEQEVLRRLEEGVDAYLERLARRIEAGFAESQVWRDVRVVCQRLRPLLQ
                     APYHGVLEVRDRNGRVIEKSEQAGIQELFCSLQRMVFELWVPTRLSTEYEKVDSFLLE
                     FMRHPRTGFRMLSQRPRQFQLITSGTNNTYMYWDYAPETASGASFLSLLFASRSLIED
                     FLSIAVLERTGLWQTQYRLVAMNTENQRAFAADDQLRVCLIPLFERARVLQRPLAGTI
                     RWRGERWVVTSLASTALQGFGLAALFPERAIEEQLRHLKYRIGLIGLVTLLLAGALAF
                     LVSRRFLQPVDELSRGVEAIQRKDFSSPVPVHGRDELGRLAQVFNLLAEELKDLDVAR
                     AVQTRMIPREYPVIEEY"
     gene            2344..2826
                     /locus_tag="PKO06_09550"
     CDS             2344..2826
                     /locus_tag="PKO06_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69931.1"
                     /translation="METRKIALALKENEELLASFYRVCIKLFPSAATEFERLAMEEDG
                     HAQLFAKVIVDIDRHPDAWRPGKISLTTLTLIGAQIRRAMEEAVSGASAPRYALTFLR
                     SAENSLGERHLEQALLTDNQEMSGYLQHVNGAFSQHLKRLDELERKLFPGPVQSIFSI
                     "
     gene            2975..3481
                     /locus_tag="PKO06_09555"
     CDS             2975..3481
                     /locus_tag="PKO06_09555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV69932.1"
                     /translation="MMTSAELLLEYRRHPKRGFDLIYQAFADRLTTYLRRSYSLSLEE
                     ISDVVHDAFLPWVANPERVAQVENISAYLFATVRNLALQVKRQRLSPIPDSFDPPVPA
                     PSAQLEESIHVEQSLQRLPDEQREAIVLKIWGDLSFEEIATIQGVPLQTAAARYRYGL
                     QKLEEMLS"
BASE COUNT          821 a         1086 c          923 g          692 t
ORIGIN      
        1 catactcctc gatgaccggg tattcgcgcg gaatcattcg agtctgaaca gcccgcgcga
       61 catcgagatc tttcaattcc tcggcgagca gattgaagac ctgtgccagg cgtcccagtt
      121 catcgcggcc atgaacggga accggcgagg aaaaatcctt tcgttgaatg gcttcgactc
      181 cccgagagag ttcatcgacc ggttgaagaa atcgtcgtga gaccaggaac gccagggcac
      241 cagccaacag cagggtcacc aggccgatca aaccgatgcg atacttcaga tgccgcaatt
      301 gttcttcgat ggcccgttcg ggaaacaacg ccgccagacc aaacccctgc agagccgtac
      361 tggcaagact cgtaaccacc cagcgttcgc cccgccagcg aatcgttccc gcgagaggac
      421 gctgcagcac gcgcgcccgt tcgaagagag gtatcagaca cacccgcaac tggtcgtctg
      481 ccgcgaaggc tctttgattc tcggtattca tcgccaccaa ccggtactgt gtctgccaca
      541 gacctgtgcg ttcgagaacc gcgatactca agaaatcttc gatcagtgac cgggacgcga
      601 acaagagaga cagaaacgaa gctcccgatg ccgtttccgg tgcatagtcc cagtacatgt
      661 aggtgttgtt cgttccagat gtgatcaact gaaactgacg gggacgctgc gacagcatgc
      721 ggaaccccgt gcgcggatgc cgcatgaact cgagcagaaa cgagtcgacc ttttcatact
      781 ccgtgctgag gcgcgtcggc acccagagtt cgaacaccat tctctgcaat gagcagaaca
      841 gttcctgaat acctgcctgt tcgcttttct cgatcacgcg accatttcga tcgcgcactt
      901 cgagtacccc atgatacgga gcctgcaaca gaggccgcag gcgttgacag acaacccgga
      961 catctcgcca cacctgcgac tcagcgaatc cggcttcgat gcgacgtgcc agtcgttcga
     1021 gatacgcatc gacaccttct tcgagccggc gcagcacttc ctgttcccca cgttcgacct
     1081 gcgttttcag cagactgcgc cgaacctcga acgacatcat ggtggcttga gccagttcca
     1141 gaagcggcac caatgctgta accaggaaga gtcctccgac caccccgcgc acgcgagcgc
     1201 ggcgctgaaa cagtgacccg gcccagccca gcatgggtat cagaaggacg acagccagtc
     1261 tcacggccca ggtctgcgac gccccttccg ccgaaggcag cggccgtgag acaaccagcg
     1321 tctctccgct gcgggtctga acaaaccaga atcctcgtga cttccactcc tgttcagacc
     1381 gtttcgtttg cagaaacgcc cgataggcct gttcgagttc cgcacgatca gtcttcgcgg
     1441 agatcaacca ctctgcgcca ggttgaagca tgccgaccca atcggagatc gagagtttga
     1501 cctgagccag agcagctcga aaccgttgtt ccggctgtgg caggtgacga cagaagatca
     1561 gcaacgccgc atctcttgtc tgttcccagg ccagatacga ttctccgccc tgaaacggca
     1621 ccgcaatcaa tcgaccacgt ccacgctgaa atcgttcgag tccgattccc aggccaaacg
     1681 cattcgacag cagacgattg atctctcggc ttttctcgcg ggtcatggat gcatcacgaa
     1741 gactcaacag caaccgccgc atcaaccaga gattctcagc ctgatcagag gcttcgagac
     1801 gcgagcccgc atcatgcaac aggaacaagt caaattgaca cccatgggtg acagagttca
     1861 gagagcgcga cagctgcgac aacgacacgc gttgattgcg ttgaaaacga cgcagagctg
     1921 ctgtcatggg gcgctggagg aacaattcag gatcggcggc aaccgccatc tgttccacgt
     1981 gttcctgcag gtgtcgcaca aaccggccgc gagtctccgt catgcgtttc tgcacctggc
     2041 tttcccagag atagccaaag gtgaacaggg gaatgatgat gaccagccat cgcgccacgt
     2101 gtgccttcat gttccctgta tattactctc ccccagtctg tctgtcagtt ctgagtacaa
     2161 gtgactccga gccatttgat tctctggctt gggttttccc cttgagcatc gctcacgcca
     2221 agcggaaaac gatacagcct caaatgaccc tgtcacagat tgcacgacgc ttgtctcttc
     2281 acgattcgcg gcgtatgatg cggttcgcgc ccgagcacta gtgaagacac cggggaggga
     2341 gagatggaaa caagaaaaat cgcgttggcc ctgaaagaaa atgaagaact gctcgcctcg
     2401 ttctatcgtg tgtgcatcaa gctgtttccc agtgcagcaa cggagtttga acgtctggcc
     2461 atggaagaag acgggcatgc ccagctgttt gccaaagtca tcgtcgatat cgaccgccac
     2521 cccgatgcct ggcggcccgg aaagatctcg ctgaccactc tcactctgat cggtgctcag
     2581 attcgtcgag ccatggaaga agccgtctcg ggagccagcg ctccgcgcta tgccctcacg
     2641 ttcctgcgtt cggccgaaaa ctctctcggc gaacgacatc tcgaacaggc gctgctgacc
     2701 gacaaccagg agatgtcggg atatctgcag cacgtcaacg gagccttttc ccagcatctg
     2761 aaacgcctcg acgagctcga acgcaaactg ttcccgggcc cggtgcaatc aattttttcg
     2821 atctgagtca ttcgccccaa ccggtctctt cgcgccaaac ggcccggaac ctgcctgtcg
     2881 cgacagtttc gaaaaaaaag agccgagttg attttgttac ataaaaacag gcttcaggca
     2941 tcacttcata gatgaacgcc aacacgaagg atgtatgatg acctcagctg aactgctgct
     3001 cgagtaccgg cgacatccga aacgcggatt cgacctgatc tatcaggcat tcgccgaccg
     3061 gctgaccacc tatctgcgcc gcagctattc tctcagtctc gaagagatca gcgacgtcgt
     3121 acacgatgcg tttctgccct gggtcgccaa ccccgagcgc gtggcccagg tcgagaacat
     3181 ctcggcctat ctgttcgcta cggttcgcaa tctggctctg caagtcaagc ggcaacgact
     3241 gtcaccgatt cccgattcat tcgacccgcc ggttcccgcg ccgagtgcgc agctcgaaga
     3301 gtcgatacat gtcgaacagt ctctgcagcg acttccggac gagcagcggg aagccatcgt
     3361 tctgaagatc tggggagact tgagcttcga agagatcgcc accattcagg gcgtgccatt
     3421 acagaccgcg gcggcacgct atcgctacgg tctgcaaaaa ctcgaggaga tgctgtcatg
     3481 aatccatcgg atcaacgccc ctcaccgatc gaggaaaaac tt
//
LOCUS       DAORHD010000405         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29753_length_3514_cov_4.416594, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000405 DAORHD010000000
VERSION     DAORHD010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29753_length_3514_cov_4.416594"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2282
                     /locus_tag="PKO06_09560"
     CDS             <1..2282
                     /locus_tag="PKO06_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69933.1"
                     /translation="REELRRLAEQQVDRGLAFHMRLVLARAEKSVTEKEPPLVRLNRL
                     LKSESIDFLAIVLECERLARRDGRVGLEMLKKAEWWNFPSETLPFMLTLLKRFGAAED
                     GARIEPLCHHHDPRVVMMAVEALEKLNPEDLRPMLVPLLSHSDSGVRGAAIRLLTKWD
                     PTEALVHFEAFLFAEDATEREIALFFAYFFPFVEVEPLLLRFLGLESDLGLLRKAGYL
                     FQVNPLPEEPPRLIEVWETCSGEKRQLIGQILTGVVTALSASGLIPKQPEEILAELQA
                     EYRSRRRHHLIEQCRVALGSTEVAHRSEGCRQLQTAIEAGHEEARQVLADHLTRETDP
                     ELRQALAAFFAVRATTTPSPVVGAPTELTPAERVTWLERVDADGYRQHRKQILSWLEQ
                     GAPAEKIPVVKLLARVGSVDDASLLKRLFGESDPALLVTLIDAVRQLDIALLDPLLPK
                     LIAHVDDQVREAAVRAFILRDKKQALIQVEQLAGSSRPSDRAIAIFALGQFDFLSVAE
                     LVVTLLERDTDPEHLRQLGVLVKARLDERLYVRVVALADKAAESKAPLFRQLADECGV
                     QLVQQSRKRFSDPAAALLWAREQLRTEESRRRAPPVYALQNIQKLRQQKTAAAEASPE
                     LIGQLLETLRQLVSTTAGQLQIAGSAVLVLLLWWSLSGDAIPEVPVQTSQSTTSAAST
                     NPRVKPFEIAEERDVQGLVNSAFSDGVLVTPDGMDEKVFVRFPSTPRPFRPGDAFRGR
                     IRIEKRNRTRYEAILMKLF"
     gene            2336..>3514
                     /locus_tag="PKO06_09565"
     CDS             2336..>3514
                     /locus_tag="PKO06_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69934.1"
                     /translation="MLCRRNRGSILIIVLGFLCCLLLYAVAYFNHMMATRRLSHRLTR
                     QQVMCETAHALATLAIHKMQYGPLLAENSGGPIPTAPGDSESLFELYRELSKPRDAMR
                     AVSGTVNLEEIRTSDLYPITRDLIEPLLKFGTFGYAVTWSCRPEDFESVGLSANRYTC
                     EKRGQIRLSVHIEYRRGDGPIQVEDHQYLARVKVAAALTPVLSRFTLYLEQVCPGVSL
                     TEQYWLNQVRNDENGETMTDSRARPLVLDHDGTDAGPHGPFTQVPRTLAELMRERRGL
                     VYLGGQSPLVLNLAMSNALRPTSSTGETCQLHRKADGFGFVDVPGRRGEAQGKPLVVR
                     EMEVGMSASPNPTARSWYDLLEKHAPNQYVDLRDRLRLEHSSIFRLLGGDTNRSPTLV
                     F"
BASE COUNT          658 a         1017 c         1101 g          738 t
ORIGIN      
        1 gccgagagga actgcgacgc ctggcggagc agcaggtcga tcggggtctg gcgttccata
       61 tgaggctggt tctggcgcga gccgaaaagt cggtgacgga gaaagaacca ccgctggtgc
      121 gactcaatcg tctgctgaag agtgaatcca tcgattttct ggcgatcgtt ctcgagtgcg
      181 aacgcctggc gcggcgagac ggccgggtcg ggctcgagat gctgaagaag gccgagtggt
      241 ggaactttcc gagcgagacg ctgccgttca tgctgacgct gctcaaacgc tttggagccg
      301 ccgaagacgg ggcgcgaatc gagccgttgt gtcaccacca cgatccgcgc gtggtgatga
      361 tggccgtcga agcactcgaa aaactgaatc cggaagatct gcgtccgatg ctggttccgt
      421 tgctgtctca ctcggacagc ggtgtgcgcg gcgcggcgat tcgcctgctg acgaagtggg
      481 atcccacgga agccctggtc catttcgagg cctttctctt cgccgaagat gcgacggagc
      541 gagagattgc gctcttcttc gcgtatttct ttccgtttgt tgaggtcgag ccgctgctgc
      601 tcagatttct cggtctcgag tccgatctgg ggctgttgag aaaggcgggg tatctgtttc
      661 aggtcaaccc gttgcccgag gaaccgcccc gcctgatcga agtctgggaa acatgttccg
      721 gagagaaaag acagttgatc ggtcagattc tgaccggtgt cgtgaccgcg ctctcggcat
      781 ccggcttgat cccgaagcag cctgaagaga ttctcgcgga acttcaggcc gaatatcgtt
      841 cgagacgccg gcatcacctg atcgagcagt gccgggtggc actcggctcg accgaagttg
      901 cgcatcgaag cgaagggtgt cgtcagctgc agacggccat tgaagccggt cacgaagaag
      961 cacgtcaggt actcgccgac cacctgacgc gtgaaactga cccggaactg cgtcaagcgc
     1021 tcgcggcgtt ctttgctgtc cgtgccacga cgacgccgag cccggtggtg ggcgcaccta
     1081 ccgagctgac tcccgccgaa cgggtgacct ggctcgaacg ggtggatgcc gatggctatc
     1141 gacagcatcg aaagcagatt ctctcgtggc tcgagcaggg agcacctgcc gaaaagattc
     1201 cggtcgtgaa actgctcgcc cgagtcggca gtgttgatga cgcaagtctc ctgaaaagac
     1261 tgtttggcga gagtgatcct gctttgctgg tcacgctgat cgacgcggtc cgtcagctcg
     1321 atattgcgtt gctcgacccg ctgcttccaa agctgatcgc gcacgtcgac gatcaggtgc
     1381 gtgaagcggc cgtgcgagcc tttatcctgc gcgacaagaa gcaggcgctg attcaggtcg
     1441 agcagcttgc cggttcgtcg cgtccttcag atcgtgctat tgcaatattt gcactcggac
     1501 agttcgattt tctgagtgtc gcggaactgg tcgtgacgct gctcgaacgt gataccgatc
     1561 cggagcacct gcggcagctg ggggttctgg tgaaggcgcg gctcgatgaa cgcctgtatg
     1621 tgcgggtggt ggcactggcg gacaaggccg cggaatcgaa agccccactt tttagacagc
     1681 tggctgacga gtgtggcgta caactggtac agcagtcccg aaaacgtttc agtgatccgg
     1741 ccgcggcatt gctgtgggcg cgcgaacagc tgcgaactga ggagtcgcgt cgccgagcgc
     1801 ctccggtgta tgccctgcag aacattcaga aactgcggca gcagaagacc gctgctgccg
     1861 aggcttcgcc ggagctgatc ggacaactgc tcgaaacact tcgacagctg gtctccacca
     1921 ccgccggtca gttgcagatc gccggctcgg ctgttctggt gctgctcctc tggtggtctt
     1981 tgtccggtga tgcgattcct gaggtgccgg ttcagacctc ccagtcgaca acgtccgctg
     2041 cctcgaccaa cccgcgggtg aaacccttcg agatcgccga ggagcgtgat gtgcaggggc
     2101 tggtcaatag tgccttctca gatggcgtgc tggtgactcc cgacggcatg gacgaaaagg
     2161 tctttgtgcg atttccctcg acaccgcgtc ccttcaggcc tggcgatgcg tttcgcggac
     2221 gcattcgcat cgagaaacgg aaccgcacac ggtatgaggc gattctgatg aagctgttct
     2281 gaggttgcgg cctgtgaatc ggaaaacgta ccagcacagg aaggtcgaac gaacgatgtt
     2341 gtgccggcga aaccgtggtt cgattctgat catcgtgctt ggttttctct gctgtctgct
     2401 tctgtatgcc gtcgcgtatt tcaatcacat gatggccacc agacgcctgt cacatcgtct
     2461 gacacgccag caggtcatgt gcgaaaccgc tcatgccctg gccacgctgg ccattcataa
     2521 gatgcagtat ggtccgctgc tggcggaaaa ctccggcgga ccgattccga ccgcgccggg
     2581 agactcggaa agtctgttcg aactctatcg cgagctctcg aagccgcgag atgccatgcg
     2641 agccgtctct ggcaccgtca atctcgaaga gatccgcacg tcggacttgt atccgattac
     2701 ccgcgatctg atcgagccgc tcctgaagtt cggcacgttc ggttatgccg tcacctggag
     2761 ctgccgcccc gaggacttcg agtcggtcgg tctgtcggcc aatcgttata cctgcgagaa
     2821 acgaggtcag atcagattgt cggtgcacat cgagtatcgt cgcggcgatg ggccgattca
     2881 ggtcgaagac catcagtatc tggcgcgggt gaaggttgcc gcggctctga ccccggttct
     2941 gagtcgtttc acgctctatc tggaacaggt ctgccccggt gtgtcgctga cggagcagta
     3001 ctggctcaat caggtgcgta acgacgaaaa cggcgagacc atgacggatt cccgcgcccg
     3061 tcctctcgtt ctcgatcatg acggaacgga cgctggtcct catggcccgt tcactcaggt
     3121 tcctcgaacc ctggcggaac tgatgcgcga acgtcgcggt ctggtatacc tcggcgggca
     3181 gtctccgctg gtgctgaacc tggccatgtc gaacgccctg cgtccgacgt cttcaaccgg
     3241 cgaaacctgt cagcttcacc gcaaagccga cgggttcggc ttcgtcgatg ttccgggccg
     3301 gcggggagaa gcgcagggga agcccctggt ggtgcgagag atggaagtcg gcatgtcggc
     3361 ctctcccaac cccacggctc ggtcctggta tgaccttctc gagaagcatg cgccgaatca
     3421 gtatgtcgat ttgcgggatc gtctgcgtct ggaacacagc tcgatcttcc gcctgctcgg
     3481 cggcgacacg aatcgctcgc ctacccttgt attc
//
LOCUS       DAORHD010000406         3513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29771_length_3513_cov_3.942452, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000406 DAORHD010000000
VERSION     DAORHD010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29771_length_3513_cov_3.942452"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /locus_tag="PKO06_09570"
     CDS             complement(<1..1027)
                     /locus_tag="PKO06_09570"
                     /inference="COORDINATES: protein motif:HMM:NF021943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69935.1"
                     /translation="MSHRRVSLIISLFSLLTLVSLVGCGGGGGGGGAVFTPIGGGFTP
                     IGSLGVAPTGFSSAAFPAADGQTQLSIANFANGSQTTVLLANNSLSPVTVQVSANTVV
                     PTNLTANVQSAVSENRVRLKVPGASFFARLRERERRAPSFSATRGAKIVANNIARADV
                     EGLDVNFAVINPETDDYSTISAKCVQRVQLSGNAYVNFFLDNSLAGTDDYNPLLQRIN
                     KYATAFAGLNGIYSVVRSVFGEEPAADFNSLGADITVLTVSMQAIGDTKEGDSHIAGF
                     FFSEDLYARSSEHPNSNQRKMFYLNANLDLSENTMISTLAHEFQHMINFYQRKKNNLS
                     EEDWLNEA"
     gene            complement(1076..2500)
                     /locus_tag="PKO06_09575"
     CDS             complement(1076..2500)
                     /locus_tag="PKO06_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV69936.1"
                     /translation="MVEKRMSDKPAVETAEKRDVSALRRRVAALRTRKGADLDTAICD
                     IIDLKGVSLAVLLEGLPRLPIRVQLAVARRVEDFFFFHPDRGRRLVGRLEKAIQKAAA
                     SCWPNLIAGLADVVARSGHVCPLTTDLRGALMAVVRSDTDLMRKGKAVEMLAHVGHRT
                     AVPDILGLMVSTQESVDDYAHFSFLETSLFALKALGGEAILRLLINPLSLPALTQFRV
                     EWRERDAEATQEVLKALQKLDDGFAQLLLKVVDLAEFNLPFTAMVMEGLEHRDKWVRQ
                     IATESFAKMKKHADIEHLMRMCQDPAPEVRLMAVQALGSFKGDLANERLMDVACNEQE
                     SYEMRMNALYALFGQKNASALESLRESPNPSIALNARGLRALLMPRDQGLKDLLDVLP
                     AGAPSRMHELFHYLMELSRPEDLTLLLEASETLKTEAQREAWIPFLQMFLKNKAGPSL
                     ETVLAGLDERQRRVLEVLRKGFVI"
     gene            complement(2533..>3513)
                     /locus_tag="PKO06_09580"
     CDS             complement(2533..>3513)
                     /locus_tag="PKO06_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69937.1"
                     /translation="PGILDDIQRMPRHRLAVFFKARIYPKAMVAYEELCKAVPGHSLY
                     VVQYARAVAVGGNYSRAVTILETAAKDYGDEAEPALALAEMFLVGREHERAVKLLERV
                     RRDFPQSVQAHFLSAYLHLQKLELEASAEAFTQVLSMAPDDEEIALWYSRLMILRGMP
                     QEAVKIWDRFQDGMESLQEIISRIELSLAAGEASKVNALLRKIGDYHPRFVEDQLLFG
                     KAFFYAGDFNGAQERFEQVLQVDGRQAEALTLLALTHLARNKPAKFWMSWQKAIESDS
                     LVPVIFGMILRGVLNFTQRERLKAETAKSLELIVRNDLDRSRLTMLLNAL"
BASE COUNT          769 a         1037 c          988 g          719 t
ORIGIN      
        1 tcgcctcatt caaccaatcc tcttcactca aattgttctt ctttcgttga tagaagttaa
       61 tcatgtgctg gaattcgtgt gctaacgtcg agatcattgt gttttcagac agatcaaggt
      121 tagcgttcaa atagaacatt tttctttgat ttgaatttgg atgctcggaa gatcttgcat
      181 acaaatcctc cgagaaaaag aaaccagcaa tatgtgagtc tccttctttg gtatcaccta
      241 tagcttgcat tgaaacggtc aaaacggtaa tgtcagcccc aagtgaattg aagtcagcag
      301 ctggttcttc tccaaaaaca cttcggacaa ccgagtaaat cccattaagc cccgcaaaag
      361 cagtcgcgta tttgttgatt ctttgcaaca agggattgta atcatccgta ccagcgaggc
      421 tattgtcgag aaagaaattc acataagcgt tgcctgacag ttgaactctt tgaacgcatt
      481 tcgcagaaat cgtagaatag tcgtccgttt ccggattgat cacagcgaaa ttcacgtcaa
      541 gcccctcaac atctgccctt gcgatgttgt ttgcgactat ttttgctccg cgtgtcgcgg
      601 aaaagctggg agctcgcctt tcgcgttcac gcaggcgggc gaagaatgag gcacctggga
      661 cttttaatcg aacgcgattc tcgctgacgg cagactggac atttgcggtc aggttcgtcg
      721 gcacgaccgt gttggctgac acctggacgg tgacggggga gagcgagttg ttggccagca
      781 gcacggtggt ctgcgacccg ttggcgaagt tcgcgatcga cagctgcgtc tggccgtcgg
      841 cagcggggaa ggccgcagat gagaagccgg tgggggcgac gccaagggag ccgatggggg
      901 tgaagccgcc accgatggga gtgaagacgg ctccgccgcc accaccgccg ccgccacatc
      961 cgacgagaga aaccagagtg agaagtgaaa aaaggctgat gatcaatgaa acacgtcggt
     1021 gcgacatgga caatttctcc tttgagacag taagcgcggg cggcggacgc ccgaactaga
     1081 tgacgaatcc cttgcgcaga acctcgagaa cacgccgctg gcgctcatcg agcccggcga
     1141 gcacggtttc gagcgacggg ccggccttgt tcttgagaaa catctgcaga aacggaatcc
     1201 aggcttcgcg ctgtgcttcg gtcttcaacg tttcgcttgc ttcgagcagc agggtcaggt
     1261 cttccgggcg actgagttcc atcaggtagt gaaacaactc gtgcatgcgg ctgggagccc
     1321 cggcaggcag cacatcgagc aggtctttca gaccctgatc acgcggcatc agcagcgccc
     1381 gcagaccccg cgcattcaac gcaatcgacg ggttcgggct ttcacgcaaa ctttcgagag
     1441 ccgaggcatt cttctgcccg aacagcgcat acagggcgtt catgcgcatc tcatacgatt
     1501 cctgctcgtt acaggccacg tccatcaggc gctcgttcgc cagatcgcct ttgaagctgc
     1561 caagagcctg aaccgccatc aagcgcactt cgggggccgg atcctggcac atgcgcatca
     1621 gatgttcgat atccgcatgt tttttcatct tggcgaacga ttcggtggcg atctgccgca
     1681 cccacttgtc gcgatgctcg agaccttcca tgaccatcgc ggtaaacggc aggttgaact
     1741 cggccagatc cacgaccttc aacagcagct gggcaaaacc atcgtcgagt ttctgcaggg
     1801 ccttgagcac ctcctgggtc gcctcggcat cacgctcgcg ccactcgacc cgaaactgcg
     1861 tcagagccgg cagagacagc gggttgatca acaggcgcag gatggcttca ccacccagcg
     1921 ctttcagcgc gaacagactc gtttcgagaa aggaaaaatg ggcgtagtca tcgaccgatt
     1981 cctgagtcga gaccatcaga cccagaatgt cgggaaccgc ggtccggtgg ccgacatgag
     2041 cgagcatctc gaccgccttt cccttgcgca tcaaatcggt atccgagcgc accacggcca
     2101 tcagcgcgcc gcgcagatcg gtcgtcagag ggcacacatg cccggagcgg gcgactacat
     2161 cggcaagacc ggcaatcaga ttcggccagc aggaagccgc cgccttctga atcgccttct
     2221 cgagacgccc caccagccga cgaccacgat cggggtgaaa gaagaaaaaa tcttcgacgc
     2281 ggcgggccac ggccagctgc acccgaatcg gcaggcgcgg cagaccctcg agcaacacgg
     2341 ccagtgaaac ccccttcagg tcgatgatgt cacagatggc ggtatcgaga tcggctccct
     2401 tgcgggtgcg cagtgccgcc accctgcggc gcaatgcgct gacatcgcgt ttttccgcag
     2461 tctcgaccgc gggtttgtcg gacattcgtt tttcgaccat gggttttcct tccggcggtc
     2521 tttccgccgc actcacaacg cgttcagcag catcgtcagg cgcgaccggt caaggtcgtt
     2581 gcgcacgatc agctcgaggc tcttggcggt ttccgctttg aggcgttcac gctgggtgaa
     2641 attcagcacg ccgcgtagaa tcatgccaaa gataactggg actaacgaat cactttcaat
     2701 cgccttctgc cacgacatcc agaacttcgc gggtttgttt cgggccagat gggtcaacgc
     2761 cagcagcgtc agcgcctcgg cctgacggcc gtcgacctgc agaacctgtt caaagcgttc
     2821 ctgcgcgccg ttgaaatcgc cggcatagaa gaacgctttg ccgaacagca gctgatcttc
     2881 gacgaaacgc ggatgataat cgccgatctt acgcaacaga gcgttgacct tcgaggcttc
     2941 accggcggcg agactcaact cgatgcgcga gatgatttcc tgcaggctct ccatgccgtc
     3001 ctggaagcga tcccagatct tgacggcttc ttgcggcata ccgcgcagaa tcatgaggcg
     3061 agagtaccag agcgcgatct cttcgtcgtc gggcgccatc gacagcacct gcgtgaacgc
     3121 ctcggccgac gcttcgagtt cgagtttctg caggtgcaga tacgccgaaa gaaaatgcgc
     3181 ctgtacgctc tgcgggaagt cgcgacgcac acgttcgagc agtttcaccg cccgttcatg
     3241 ttcgcgtccg accagaaaca tttcagccag cgccaacgcg ggttcagcct cgtcgccgta
     3301 gtctttcgcc gccgtttcca gaatcgtgac ggcgcgcgag tagttgccgc ccacggccac
     3361 ggcccgggca tactggacga catacagcga atgccccggc accgccttgc agagttcttc
     3421 gtaggcaacc atcgccttgg ggtagatacg cgccttgaag aacactgcaa ggcggtgccg
     3481 gggcattcgc tgtatgtcgt ccagtatgcc cgg
//
LOCUS       DAORHD010000407         3511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29802_length_3511_cov_4.072627, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000407 DAORHD010000000
VERSION     DAORHD010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29802_length_3511_cov_4.072627"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..3019)
                     /locus_tag="PKO06_09585"
     CDS             complement(140..3019)
                     /locus_tag="PKO06_09585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69938.1"
                     /translation="MKRTFVQLWDSLRNSLLVAVLLALPLLLVHEGLEVRRLEMLHLN
                     RLEDEQALDKMSNHFQADNNLRLIAQVLGEIEAVAKRGSDPVSELREQMRELKRRHPG
                     VFETLMFDSQAKPVAGFDTFPEWKELIASMSDCLYRFYRGDTDTLRSTFHKLTPIVGP
                     FIAGGFEISVARGMYLRSFSAHRQYLFHSGLWYGKEPQHLVMFFVSVTQEFPQLGFNL
                     VRSMLRRWYSEAIVELLDLERPVSRWPIPRNLSRFALKRQLIEKDAADTRAWFDQGRI
                     WRQVAFSSHQRIVYSLPESGYERAQREAERNRLLLLCAWGLCFAGFRLLQTTFADLSL
                     RVRLLVLFGFATGIPLLLLGYTAVGLVQDRRENLRKKAFAESGILLEQFAGEILVAFG
                     RFERTVRQFTTRRLTATDEGVASAAAEFESIRRETGAVAALLSDGDRTPVYQYVTPEK
                     TRGGGGFEGVYRTLAPLFSLQFAQRMAALNQEQRPFLETARDQAMKATLESYGIELDQ
                     LLCRFEDSVNRLFVMNLGGERTGLISFFLRSVSGARRLGGIVYWSQPLLNRLDEVAAE
                     RMRQRLPDWRIDTHEQTSLAERQDPFAKAVRKLAMRMNDRVLSHERILLGSESWLLSG
                     NWFDSLVPVSLYCARLEQPVVAGLRHTTRGLFLVALLLLASGCTIALLLSRHLLSSVR
                     HLEIGMEAVKRRDFRERIPVETADELGRLASTFNQVMDGLGDLEVAKIVQETFYPSEP
                     LAEQGWQVAGACQTAGRVGGDYFDHLALPDGRWLLLIGDVSGHGTAAALVVALAKAVV
                     CHPMTPTEPSQILQMLNEQLVQSVGRRKMMTCFVALFDPRTGRLEASNAGHNYPMLVQ
                     RQTVEELRIEHLLLGARSRQPFPAEHRQMSEQAFLCFYSDGLVETLDRQGQQIGYDRL
                     SQQLPHLLRPTAPETVQAILDWRSGLAVSETLEDDVSVMVLQRFA"
     gene            complement(3016..>3511)
                     /locus_tag="PKO06_09590"
     CDS             complement(3016..>3511)
                     /locus_tag="PKO06_09590"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV69939.1"
                     /translation="AVCGDPEKLVQTMNTLFLNVLRKKKMMTCCVGLVDTCDSTLRLI
                     NAGHPWPLQVRGGCAEYLKSVGMPLGSAKRWKAGEVTWRLDPGDVVFLYSDGLVEARA
                     GNDVLGFSRLSESLPQLLRSEPRETIAAFWSWFDHMAPESPPADDVSVLVIQRQPASG
                     EVRA"
BASE COUNT          834 a         1066 c          939 g          672 t
ORIGIN      
        1 tccgcccgcc tcttgccgaa cggctcacaa gtttcgctct tgcgaacgga aaatgagcga
       61 gaattcaatg accctgtccc aagcgccaga gaaaaaggga actgcacctg cgtgtgaggg
      121 gtgtttgggc ttacgtttat caggcgaagc gctgcagaac catgactgaa acgtcgtctt
      181 cgagggtttc tgagaccgcg agccccgaac gccagtcgag aatggcctgt acggtttcgg
      241 gggcagttgg acgcagcaga tgcggtaact gctgactcaa acggtcgtag ccaatctgct
      301 gcccctgtcg gtcaagcgtt tccaccaaac cgtcggaata gaagcagaga aaagcctgct
      361 ctgacatctg tcgatgctcg gccggaaagg gctgacgtga tcgcgctccc agcagcaggt
      421 gttcgatgcg cagctcctcg acggtctgcc gctgaaccag catcgggtag ttatgcccgg
      481 cattcgaagc ttcaagtcga ccggtacgcg gatcgaacag ggcaacgaag caggtcatca
      541 tcttgcgtcg cccgacactt tgtaccagtt gttcgttgag catttgcagt atctgtgatg
      601 gttctgttgg agtcatcgga tgacacacga cggctttggc aagcgcaacg accagggcgg
      661 ccgccgttcc atgccccgac acatcgccga tcagcagcag ccaacggcca tcaggaagag
      721 caagatgatc gaaatagtca cccccgactc gaccggccgt ttgacaggct ccggcaacct
      781 gccagccctg ttcggccaaa ggttcagatg ggtagaacgt ctcctgcaca atcttcgcca
      841 cttccagatc gccgagcccg tccatgacct ggttgaaagt cgaagccaat cgtcccagtt
      901 catcagcggt ttcaaccggg attcgttcac ggaaatcgcg acgtttcacc gcctccatgc
      961 cgatttcgag atggcgtaca gagctcagca gatggcgtga cagcagtaac gcaatcgtac
     1021 aaccagaagc gagcagcaac agagcgacca ggaacaagcc tcgagtggta tgtcgcaacc
     1081 cggcgacaac cggctgttcg aggcgagcac aatacaggct gaccggcacc agagaatcga
     1141 accagtttcc cgacaagagc caggattcgc ttcccaacag aatgcgttca tgagacagca
     1201 cccggtcatt catgcgcatc gccagctttc gaaccgcctt ggcgaacggg tcctgacgtt
     1261 ctgccagact cgtctgctcg tgcgtatcga ttcgccagtc cggcaatcgc tggcgcatcc
     1321 gttctgccgc gacctcgtcg agccggttca gcagcggttg cgaccagtag acgatgccgc
     1381 ccaaccgtcg cgcaccagac accgagcgca ggaaaaagga aatcaaacct gtccgttctc
     1441 cgcccagatt catgacgaac agccgattta ccgaatcttc gaagcgacac agcagctggt
     1501 ccaactcgat gccataagac tcaagtgtcg ctttcatcgc ctgatcgcgt gccgtttcga
     1561 gaaatggtcg ctgctcctga ttcaatgcgg ccattcgttg cgcaaactgc agcgagaaga
     1621 ggggggccag cgtacggtag accccttcaa atccgccccc accgcgagtt ttctcaggtg
     1681 tgacgtactg gtagaccggc gttcgatcac cgtcgctcag taatgcggca acagcgcccg
     1741 tctctctgcg aatggactcg aactcggcag cggccgatgc caccccctcg tccgtcgccg
     1801 tcagacgacg ggtggtgaac tgccgcacgg ttcgttcaaa gcgaccgaac gcgaccagaa
     1861 tctcaccagc gaactgctca aggagaattc cgctttcggc aaacgctttt ttgcgtagat
     1921 tttcacgacg gtcctgtacc agtccgacag ccgtgtaacc gagaagcagc aaaggaatgc
     1981 cggttgcaaa tccaaacaac accaacaatc gaacccgtag ggacagatcg gcgaacgtcg
     2041 tctgcagcag gcgaaatccg gcaaaacaca agccccaggc gcagagaagc aacagacggt
     2101 tgcgttccgc ctcacgctga gcccgctcat acccggattc ggggagcgaa tacacgatac
     2161 gctgatgaga actgaacgcg acctgtcgcc agattcttcc ctgatcgaac caggcccgcg
     2221 tatcggccgc gtctttttcg atcagttgtc ttttcaaggc aaagcgactg aggtttcggg
     2281 gaatcggcca gcgcgacact ggtcgttcga ggtcgagcaa ctcaacgatc gcctcgctgt
     2341 accaacgacg cagcatgctg cgcacgaggt tgaagccaag ctgggggaat tcctgcgtca
     2401 ccgagacaaa aaacatgacc aggtgctgcg gctctttgcc gtaccagaga cccgagtgaa
     2461 agagatactg ccgatgggcg gaaaacgatc tgagatacat gccacgtgcg acactgatct
     2521 cgaatcctcc cgcgatgaac gggccgacaa ttggcgtgag tttatgaaag gtgcttctca
     2581 acgtgtccgt gtcacctcgg taaaaccggt acaaacagtc gctcatcgag gcgatcaatt
     2641 ccttccactc aggaaaagta tcgaatccgg caaccggttt cgcctgcgag tcaaacatca
     2701 gagtctcaaa gaccccggga tgacgacgtt tcaactctct catctgttct cgcaactcgg
     2761 acaccggatc agaaccgcgt tttgcaaccg cttcgatctc gccaagtacc tgcgcgatca
     2821 gacgaagatt gttgtctgcc tgaaagtgat tgctcatctt gtccagggct tgctcgtcct
     2881 caagtcggtt cagatgcagc atttcaagtc ttcgaacttc cagaccttcg tgcaccagca
     2941 gcagtggaag cgccagcaaa accgcgacca ggagactgtt tctcaaactg tcccagagtt
     3001 gaacaaaggt gcgtttcacg cgcgcacctc ccccgaagca ggctgtcgct gtatgacaag
     3061 taccgacaca tcatcagccg gcggggattc cggagccatg tgatcgaacc aggaccagaa
     3121 tgcagcaatg gtttctcgcg gttcagagcg cagaagctgc ggcagcgact cggacagtcg
     3181 cgaaaaaccc aacacgtcat tcccggcccg agcctcaacc agaccatcag aatagagaaa
     3241 gacaacgtcc cccggatcga gtcgccaggt gacttctccc gccttccaac gcttggcgct
     3301 gcccagtggc atcccaacgg acttgagata ctccgcgcaa ccaccgcgca cctgcaaagg
     3361 ccacggatgt cctgcattga tgagccgcag cgttgaatcg cacgtatcga cgaggcccac
     3421 acaacaggtc atcatcttct tcttgcgtaa cacgttcaga aagagcgtgt tcatcgtctg
     3481 caccagtttc tcgggatcac cacagaccgc g
//
LOCUS       DAORHD010000408         3504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29876_length_3504_cov_4.011308, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000408 DAORHD010000000
VERSION     DAORHD010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29876_length_3504_cov_4.011308"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKO06_09595"
     CDS             <1..826
                     /locus_tag="PKO06_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69940.1"
                     /translation="EELQHELERRLGLAMSVTFRKVSGDRNLDEIPSGTWLLTEVDDE
                     DPVVLPGLMPLPLTVELRWMLSLWRTPLEQTGEISLPIADLPSFLATCRLLRSRSPST
                     FPWFQGLYDPGTLCRLEEALGKSRLQTRGSGSGAGGLLAVLNLAVEENLLNPLSLEAD
                     SALAFEVFEAGDCVMTGMWVLPSMVAPTSTLRQRLPTLAFQPFPRPIGGAQVPSLSLR
                     LFAPEALIPQIASESFEVDQMSASATRIMRVQVRDDRDWEKQQYPQLYNRLIRGEY"
     gene            836..2989
                     /locus_tag="PKO06_09600"
     CDS             836..2989
                     /locus_tag="PKO06_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV69941.1"
                     /translation="MFISLRIKILGLLILLVGILAIVFTERASELIRLKMTETFEDHL
                     AGVSQGVRAFWDRESKQIIRTAGLYSESEKVVSYSLYGLHNLLQREIRRLIAGTGYFD
                     IEMQLKSGLTISASADGFFRGSALDPRDPQLNLTRVVIDKVDHFMELRARVPILKLGD
                     LIGLLTLRRRISDEVLREVGRMLQVELAIAVKGEILATSLPLSQRSEFLKNLYDRVSR
                     PQSAFTMQIGGIPHSITLIDIGETPKHDPVAIYAILSQGKMQQMIEEARAQTIRITAV
                     ALTLALIFSIVFAEFVLTSRLRRLRDSSQTIAQGDLSVRIPVDTGDELGDLARSFNEM
                     TSNLQENRNRLVQNNDELQRSLETLETLKSYIQDILSSLNTCVLTLNRQGRVETVNEA
                     AKRELAAVYPTLVGLSLRSFLKGLQKKERVLFIGALKAFQRSGGTAPPFDLEFESPAH
                     GVKVMQANLANLRDLRGTAYGLILTLEDITQRKIIEKQLYHADKLSSIGQLAASVAHE
                     IKNPLASIKTLGQLLQEETDSGDTRREYIDVIVSEVNRLNGVVEQLLRYARPEESSFR
                     VIKLSELLQPVTALIHHELERHRVQLHVEMPEEVLVLVDGEKIKQVFLNLIFNGIQAM
                     PDGGMLTLKAWQDQATPWTICQISDTGSGMSEEVVKRVFEPFFTTKQRGTGLGLAIVK
                     KIIDLHGGTIEVTSRPGEGTTFTFYLPRERKEQES"
     gene            2986..>3504
                     /locus_tag="PKO06_09605"
     CDS             2986..>3504
                     /locus_tag="PKO06_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69942.1"
                     /translation="MTLRSNNILIVDDEKAILFSLKAALQKEGHLVKTMTSPQEALKM
                     IEPGAFQVIISDYNMPAMNGMEFLRAVKKLDAEAVFILMTAYGSEKLAIEAMKEGAYD
                     YFTKPFDIEEMRIIVAKACERFSLAWQKKNLEERFLVEPEKDRIIGTSPQTQAVKGQI
                     EQVARSEISILIQ"
BASE COUNT          759 a          938 c         1028 g          779 t
ORIGIN      
        1 cgaagagttg cagcacgaac tcgagcgccg gctgggcctt gccatgtcgg tgacttttcg
       61 aaaggtcagt ggcgatcgga acctcgatga gattccctca ggcacctggt tgttgaccga
      121 agtcgatgac gaagatcccg tcgtgcttcc cgggctcatg cccctgccgc tgacggtcga
      181 gttacgctgg atgctgagcc tgtggcgcac cccgctggag cagacgggag aaatctcgct
      241 gccgattgcc gacctgccct cgtttctcgc gacctgtcgc ttgttgcgca gtcgttcccc
      301 ctccaccttt ccctggtttc agggcctgta tgatccggga accctctgcc ggttggaaga
      361 agccctcggg aaaagcaggc tgcaaacacg tggcagcggg tctggcgccg gcggattgct
      421 ggccgtgctc aatctggctg tggaagagaa cctgctgaac cccctctcgc tggaggccga
      481 cagcgctctg gcgttcgagg tgtttgaggc gggtgactgc gtgatgaccg ggatgtgggt
      541 gttaccatcc atggttgctc cgaccagtac cctcagacag cgattaccca cgcttgcttt
      601 ccagcccttc ccccgcccca tcggcggggc acaggttccg tctctgtctc tgcgtctgtt
      661 cgctccagag gctttgattc cgcagatcgc cagtgagtcg tttgaagtcg atcagatgtc
      721 agcttcagcg actcgtatca tgcgcgtcca ggttcgcgat gaccgcgatt gggaaaaaca
      781 gcagtatccg cagctgtaca atcgcttgat tcgcggggag tactgagatc ggactatgtt
      841 catctctctg cgcatcaaga tcctggggct gttgattctg ctggtcggca ttctggccat
      901 cgtctttacc gaacgggcat cagagttgat tcgcctcaag atgaccgaaa cgttcgagga
      961 ccatctggcc ggggtcagtc agggcgttcg tgcattctgg gatcgcgaaa gcaaacagat
     1021 tatccgcacg gccgggttgt actccgagtc ggaaaaggtt gtcagttact cattgtatgg
     1081 tctgcacaat ctcttgcaaa gagagatacg ccgacttatc gctggtacag gatacttcga
     1141 tatcgagatg cagctcaagt caggcttgac gatctcggcg tcggccgatg gcttctttcg
     1201 cggatcagcc cttgacccgc gcgacccaca gctgaacctg acccgcgtgg tgatcgacaa
     1261 ggtcgatcac ttcatggaat tgcgggcccg ggtgccgatt ctcaaactcg gcgatctgat
     1321 cggtctgttg actctgcgac gccgcatcag tgacgaggtg ttgcgcgagg tcggccgcat
     1381 gttacaggtt gaactggcga ttgccgtcaa aggagagatt ctcgcaactt ctcttccgct
     1441 cagtcagaga agtgagtttt taaagaactt gtacgatcgc gtgagtcgac cccagagtgc
     1501 cttcaccatg cagattggcg gcatccccca ctccatcacc ttgatcgata ttggcgaaac
     1561 gccgaagcat gatccggtgg cgatctacgc aattctgtcg caaggcaaga tgcagcagat
     1621 gatcgaggag gcgcgtgccc agacgattcg catcactgct gtcgcgttga ccctggcgtt
     1681 gatcttctcg atcgtgtttg ccgaattcgt gttgacatcg cgtctgcgtc gactgcgcga
     1741 cagctcgcag acgattgccc agggcgatct gagcgtgcgc attccggtcg ataccggcga
     1801 cgagttgggc gatctggctc gctccttcaa tgaaatgacc tcgaacctgc aggaaaaccg
     1861 caaccgcctg gtgcagaaca acgatgagct gcaacgttcg ctcgagacgc tcgagacgct
     1921 gaagagttac attcaggaca ttttgagcag cctcaacacc tgcgtgctga ccctgaatcg
     1981 ccagggtcgc gtcgagacgg tcaacgaagc ggcgaaacgc gaactggcag ccgtgtatcc
     2041 gacgctggtt ggtctctcgc tgcgcagctt cctgaaggga ttgcagaaga aggaacgggt
     2101 attgttcatc ggggcattga aagcctttca gcgttcaggg ggaaccgccc cgccctttga
     2161 tctggaattc gagagccctg ctcatggggt gaaggtcatg caggccaatc tggcgaatct
     2221 tcgcgatctg cgcggaaccg cgtatggact gattctgacg ctcgaagata ttacacagcg
     2281 aaagattatc gagaagcagc tgtatcatgc ggacaagctc agttccatcg gccagctggc
     2341 ggccagcgtg gcgcatgaga tcaagaatcc gctggcttcg atcaagacgc tgggccagct
     2401 gcttcaggag gagaccgatt ccggagatac gcggcgcgag tatatcgatg tcattgtctc
     2461 ggaagtcaat cgtctcaatg gcgtggtcga acagctgctg cgctatgcgc gtcctgaaga
     2521 atccagtttc cgtgtcatca aattgtccga gttgttgcag ccagttacgg ctctgattca
     2581 tcatgagctc gagcgtcatc gagtacaatt gcatgttgaa atgccagaag aggttctggt
     2641 gctggttgac ggcgagaaga tcaaacaggt atttttgaat ctgattttca acggcattca
     2701 ggccatgccc gatggtggca tgctgaccct gaaagcctgg caggatcagg ccaccccctg
     2761 gaccatctgt cagatctcag acaccggatc ggggatgtca gaggaagtgg tgaagcgcgt
     2821 cttcgaaccg ttcttcacga ccaagcagcg aggcacgggt ctggggctgg cgatcgtgaa
     2881 gaagattatt gacctgcatg gtggtactat tgaggtgacg agtcgaccgg gtgagggaac
     2941 cacgtttacg ttctatcttc ctcgtgagcg aaaggaacag gaatcatgac attgcgtagc
     3001 aacaacatcc tgatcgtgga tgacgaaaag gccattctgt tcagtctgaa ggcggctcta
     3061 cagaaagaag ggcacctggt gaaaaccatg acgtcgccac aggaagctct gaagatgatc
     3121 gagccgggag cgtttcaggt catcatttcc gattacaaca tgccagccat gaatggcatg
     3181 gaattcttgc gcgcggtgaa gaaactggat gccgaagcgg tattcattct catgacggcc
     3241 tacgggtcag agaagctcgc aattgaggcc atgaaagaag gagcctacga ctacttcacc
     3301 aaaccctttg atatcgagga aatgcgtatc attgtcgcca aggcctgcga gcgtttttcc
     3361 ctggcctggc agaagaagaa cctcgaggaa cgttttctgg tcgagccgga aaaggatcgg
     3421 atcattggga cttcgccaca gacacaggcc gtgaaggggc agatcgaaca ggtcgcacgc
     3481 agcgagatca gcattctcat tcaa
//
LOCUS       DAORHD010000409         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29909_length_3501_cov_3.170923, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000409 DAORHD010000000
VERSION     DAORHD010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29909_length_3501_cov_3.170923"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="PKO06_09610"
     CDS             complement(<1..1680)
                     /locus_tag="PKO06_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017050708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV69943.1"
                     /translation="MPKTIVIVGGVAGGASAAARARRLSEEANIIMLERGPYVSFANC
                     GLPYHIGGVIKEWDALQVQTPESLKARFNLDVRVLHEATRIDRQNKRLQVRDLKNQRD
                     YSLDYDYLILSPGAEAFKPPIPGIDSPNVFTLKTIPDMDRIIDWMNQHPVKHATVVGA
                     GFIGIETAENFRHRGLAVTLIEREKQVMPPMDPEMTALLHQHLRFNGVDLRLGSTVTG
                     ISATGQRLGIQFDSGTPLETDLVVLSIGVRPESKLAKEAGLALNERGAIKVNEVMETS
                     DPAILAVGDAIEVSDFVTETQVMMPLAGPANRQGRIAASKIFGEDIPYRGTTGTWVVK
                     VFDLTAAQTGPSEKTLKRLGKPYAKVYLHPSSHAGYYPGAAPMAFKLLYDPQNGRVLG
                     AQIVGTDGVDKRTDVIATAVAAKMTVSDLHHLELCYAPPYGSAKDPVNLAGMIADNLM
                     KGSHQGLTPDQLQDLKPNSYVLIDVRTALEHEAGHIPGSQHIPVDELRNRLGELPKDK
                     EIILYCQVGLRGYVANRMLTQKGIKNRNLVGGYKTWAMFHEERLSSSIPAQI"
     gene            complement(2199..2675)
                     /locus_tag="PKO06_09615"
     CDS             complement(2199..2675)
                     /locus_tag="PKO06_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69944.1"
                     /translation="MVNRIDGAGNKVIIVTKPKPKESAEKSKEGGFDKVLTGRGSEAT
                     KTATGRLAESPPGMQPQNLSHMQRLEEIARQVKDGTYALIDPTVLADRIMKIASNKEL
                     RAKFIKKLLAEEADTAKAKNRPLTELDLKKLILMVKDSNDETFEDEELEKLLKDLA"
     gene            2871..>3501
                     /locus_tag="PKO06_09620"
     CDS             2871..>3501
                     /locus_tag="PKO06_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69945.1"
                     /translation="MIRCRSFLIFALLLIMQQELLPQAFASAVKQRSSSVNDRFTQAE
                     AAFRVNNLAVAEQVYTEIVARQPGHVKARFRLGQVLTLLGKSQQALPHLEELLRQNPG
                     HVTGRCFLARVLADIGRVQEATGHLEWILQVQPEHEEARTMLTQIRADAPSGTSSGSV
                     GQRAGNVTHEGFEPMPSTVNASVDGSMGQIAPKGPQPIMLGQGTVPPSAD"
BASE COUNT          766 a          897 c          990 g          848 t
ORIGIN      
        1 gatttgagcg ggaatgcttg aggaaagccg ctcttcgtgg aacatggccc aggtcttgta
       61 accgcctacc aggttacgat ttttgatgcc tttctgcgtg agcatgcgat tggcaacata
      121 accccgcaat ccaacctgac agtacaagat gatttcttta tctttcggca gttcaccaag
      181 tcggtttcga agctcgtcca cgggaatgtg ctgggatccc ggaatatgtc ccgcttcatg
      241 ctccagagct gtacgaacat cgataagtac gtaggaattg ggtttcagat cctggagttg
      301 atcgggggtg agtccttggt gagacccctt catcaagtta tccgcgatca tgccagccag
      361 attgaccggg tcctttgccg agccgtacgg aggggcgtaa cacagttcca gatgatgcag
      421 atcgctgacg gtcatctttg ctgcgaccgc ggtagcaatg acgtccgtgc gtttgtcgac
      481 gccatccgta ccgacgatct gagccccgag aacgcggcca ttctgcgggt catacagcag
      541 tttgaaggcc atgggagctg cacctgggta atatcccgcg tgacttgacg ggtggaggta
      601 gactttggca tagggctttc ccaggcgctt caaggtcttc tcggaaggac ctgtctgtgc
      661 ggcggtgaga tcgaagactt tgacgaccca cgttccggtt gtgccccgat agggaatgtc
      721 ctcaccaaaa atcttcgagg ccgcgatgcg cccctggcgg tttgccggtc cggccaaagg
      781 catcattacc tgggtttccg tgacgaaatc actgacttcg atggcatcac cgactgccag
      841 aatcgccggg tctgacgttt ccatcacctc gttaactttg atggctccgc gctcattcaa
      901 ggcgagaccg gcttccttgg ccagtttgct ctctgggcga acaccgatcg agagaacaac
      961 caggtcggtt tcgagagggg taccagagtc gaactgaatt cccagccgct gaccggtggc
     1021 tgagattccg gtgacggtac tgcccaatcg gagatcgact ccgttgaatc gcaggtgctg
     1081 gtgcaagaga gcggtcattt ccgggtccat ggggggcatg acctgctttt cgcgttcgat
     1141 cagtgtgacc gccagtccgc gatgtcgaaa attttccgct gtttcgatgc cgatgaatcc
     1201 ggcgccgacg acggttgcat gcttgacggg gtgctggttc atccagtcga tgatccgatc
     1261 catgtccgga atcgtcttga gtgtgaagac gtttggagaa tcgatgccgg gaataggcgg
     1321 tttgaaagct tcagccccgg gcgagagaat gagatagtca tagtcgagcg agtagtcgcg
     1381 ctgattcttg agatcgcgca cctgcaggcg tttgttctgc cgatcgattc tggttgcttc
     1441 atgcaggaca cggacatcga gattgaaacg agctttcaat gattcaggtg tctggacctg
     1501 gagagcgtcc cattccttga tgacacctcc gatatgatag ggcagtccac aattcgcaaa
     1561 gctgacgtac gggccacgtt cgagcatgat gatgttggct tcttcagaca ggcgccgggc
     1621 acgagctgca gctgacgcgc cgccggcaac accaccgacg atgacaatgg ttttcggcat
     1681 agagagatct cctttactga atctgttcga gatgaaggga gcggaaaata ttcggggtgg
     1741 cttcaagcat gagcatcaac gttacagttc gtttggcagt ggcaaagaca tttgaagagg
     1801 ttgtacaaac cgggctgagc cagttgatac agggccgaag tgccatctcg gcggcaacgc
     1861 acgacgcctc cggatttcat gatgcgaaga tgttgtgaga cggaggtcta cctcgctctg
     1921 aatctgggaa acactacgct caccagttgc gagtgcctcg aagatgcgca gtcgttgcgg
     1981 gtggccaaga gacttgagca cctgtgcaat ctggtcgaga tagcgcggct tgaaatgaag
     2041 tgtagtattc atatatatga ataataatat ataaagatat aaaaatcaag cgggcaaaaa
     2101 cagatacagg aacagaggca caaacgcagg aacagaagcg aaaaagaacg ccggcggaca
     2161 gagtgactgc ccgccggcgc gcgatgtttt gagtgacttc aggccagatc tttcagcagc
     2221 ttttcgagtt cctcgtcttc aaaggtttcg tcatttgaat ctttgaccat caggatgagc
     2281 ttcttcagat cgagctctgt gagaggccgg tttttggctt tggcggtatc cgcctcttct
     2341 gccaggagct tcttgataaa cttcgctcgc agttccttgt ttgacgcgat cttcatgatg
     2401 cgatcggcga gaaccgtcgg atcgatcaga gcataggttc catctttcac ctgccgcgcg
     2461 atctcttcga gtcgctgcat gtgagacaaa ttctgtggct gcattccggg tggactttct
     2521 gccagacggc cggtagccgt cttggttgcc tcagaccctc ttccggtgag tactttgtcg
     2581 aagccgccct ctttcgactt ttccgccgat tcttttggct tcggtttggt cacaatgatg
     2641 accttgttcc ccgccccatc aattctgttt accatacgac atctcccttc acatccgtgt
     2701 gctgggcatt atccacccgt ttatcgtcac tatacttatc gtcattgaga ccagaaaact
     2761 ttacccctcc acaccatttc tttttcggcc agaaaaatca accctgtctg gagtcctgcc
     2821 gataatgtga agagaactat cagaaattcc ctgtagaggg aggcacgata atgatccgat
     2881 gcagaagttt cctcattttt gctctcctgc tgatcatgca gcaggagttg ttgccccagg
     2941 cttttgccag tgcggtcaaa cagcgctcga gcagtgtaaa tgatcgattc acccaggccg
     3001 aggctgcctt tcgtgtgaat aatctggcgg tcgcggaaca ggtgtatacc gagattgttg
     3061 ctcgtcaacc gggccacgtc aaggctcgat tcaggctggg acaggttttg acgcttctgg
     3121 ggaaatcaca gcaggcgtta ccgcatctgg aagaactgct gcggcagaat cctggtcatg
     3181 tgacaggacg ctgttttctg gcccgagttc tggcggatat cgggcgagtt caggaagcca
     3241 ccgggcattt agaatggatt ttgcaggttc aaccggagca tgaagaggct cggacgatgc
     3301 tgacgcagat acgagcagat gcaccgtcag gcacaagctc tggcagcgtc ggtcaacgag
     3361 cgggaaatgt cactcacgag gggttcgaac ccatgccctc gactgtgaat gcttcggtcg
     3421 atggctcgat gggacagatt gcgccaaaag ggcctcagcc gatcatgttg gggcagggta
     3481 ctgtccctcc cagcgctgat g
//
LOCUS       DAORHD010000410         3498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29942_length_3498_cov_2.778100, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000410 DAORHD010000000
VERSION     DAORHD010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29942_length_3498_cov_2.778100"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..1683
                     /locus_tag="PKO06_09625"
     CDS             109..1683
                     /locus_tag="PKO06_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV69946.1"
                     /translation="MAMMRSMFRWLALAMVLSMVVVPGWAQSSSSATGKVLFILLDGC
                     RADIFHRLCDEGKTPTFAWMRKNGLSVDHAVSVFPSTTGPAYAPFLCGTYPYQSNLTG
                     IRQYLRNSGTYRSYCGTDVKKITEDLSRNHPTIFELLPNDSLAVVAMLDRGAKRSFNP
                     SIDYFIKSYRGKYLECDRSIFKKLQKEIADGLPRYTFVSFHGPDSVGHRSGADGVTYD
                     ETIINLDRIVNELVHKLKARGELEQTTIILSADHGSQSTDKSGDLAPVLAGMGLKVQD
                     AIGRSTFGYNFDKNGHARENDVIVCVSGNACVQVYLKGRPETDPDAEPSFKTRPTLTQ
                     MRRYQRGKDSREVGDVIATLLKQPCVGFLAVRNGRNSYQVLSREGTGLITRDNDRLGY
                     KVIAGSDPLKMSAQARQNSDGRLIQERKWLRMTCADPYPDAVFQIAQLLEAENSGDIL
                     INAAPGYEPWHEGQKGVHGGIDAAQLKVPLVLYGRGITPGRLPCARTVDVYVTILNLL
                     DIEHPRGLLGVPLLEQ"
     gene            1742..2620
                     /locus_tag="PKO06_09630"
     CDS             1742..2620
                     /locus_tag="PKO06_09630"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV69947.1"
                     /translation="MKHGWKLAVGAAFVVAGLWVAGNWFVNSALPVGILSAQHWHESL
                     QSRLERDRSGLETLNHEQLTPLQTAVKSGCITCATLLLRAGADPEKPLAGLLNLGVRP
                     LHLAATAGDPEMVKLLASSGAPLNATDSVGDTALHAAVHWKKEGAAKTLLELGADPNV
                     ADANSWGPLHWAALWGHDTLVPALVQAGARIDQPVRVKYLLEEFVQPQRDRMVGYLHL
                     TMWPDLEGKLPARQQKYPLFYWHNRPKEKLGAEQAEVDPSPRLMTPLDLSRLLNRESV
                     AAYLESQLAKKAPARE"
     gene            complement(2812..3351)
                     /locus_tag="PKO06_09635"
     CDS             complement(2812..3351)
                     /locus_tag="PKO06_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69948.1"
                     /translation="MNNTVKSLVLLLFLSLIALPAFSSPVDELLSISATYELTVLQPL
                     PEAVTEELKLSEQESKLTEQILADQQETEKLTKKANLLSQGMIRRLLNRVQFKINHEN
                     RVELKGVMTALSGMSEQMMRGKKKIAYIAGREVNLLDGEWREAPDGRQYWVSNEYPEI
                     ILSPAEYRKYAATAVIVTD"
BASE COUNT          735 a          980 c         1031 g          752 t
ORIGIN      
        1 ggaattctgt ctggtctctg ggacagatct cggaatattc aggtataatg aatcaataac
       61 gctcgagatc tgcatgattt tgcaacgtga gcttgcgagg agggcagtat ggcaatgatg
      121 cgatcgatgt tccggtggtt ggctctggcg atggtgttga gcatggtcgt ggtgcccgga
      181 tgggcgcaga gttcgtcgag cgcgaccgga aaagtactgt tcattctgct cgatgggtgt
      241 cgtgctgata tctttcaccg gctctgcgat gagggcaaga caccaacctt tgcctggatg
      301 cggaaaaacg gactctcggt cgatcacgcc gtgtccgtgt tcccgtcgac caccggcccg
      361 gcctacgccc cgtttctctg cggtacctac ccctaccagt cgaatctgac cggcattcgc
      421 cagtatctac gaaactccgg tacctatcgc agctactgcg gcaccgatgt gaagaagatc
      481 accgaagatc tttcccggaa ccacccgacc atcttcgaac tgctgcccaa cgacagtctg
      541 gccgttgtgg ccatgctcga tcgaggagcc aagcgctcgt tcaatccgtc gatcgattac
      601 ttcattaaaa gctatcgcgg caagtatctc gagtgtgacc gctcgatctt caagaagctg
      661 cagaaagaaa tcgccgacgg cctgccccgc tacacgttcg tcagctttca tggccccgat
      721 tcggtcgggc atcgctccgg cgctgatggc gtgacctatg atgaaacgat catcaacctc
      781 gatcgcatcg tcaacgaact ggtacacaag ctgaaagccc gcggtgaact ggaacagaca
      841 accatcattc tcagtgcgga tcacggctcg cagtcgaccg acaagagtgg agacctcgct
      901 ccggtgctgg ccggcatggg gctcaaagtg caggatgcca tcggccgctc gaccttcggc
      961 tacaacttcg acaagaacgg acatgcgcgc gaaaacgacg tcatcgtctg tgtgagcggc
     1021 aatgcctgcg tgcaggtgta tctcaagggg cgcccggaaa ccgatcccga cgccgaacct
     1081 tctttcaaaa ctcgcccgac cctgacccag atgcgccgct atcagcgtgg aaaggactcg
     1141 cgcgaggtgg gtgatgtgat cgcaaccctg ttgaaacagc cttgcgtggg ttttcttgcc
     1201 gtgcgcaacg gccgcaattc gtatcaggtg ctctcgcgtg agggaacggg gttgatcacc
     1261 cgcgacaacg atcgcctggg atataaagtc attgccggtt cagatccgct gaagatgagt
     1321 gcacaagctc gccagaactc ggacggtcgc ttgattcaag aacgcaagtg gctgcgcatg
     1381 acctgcgccg atccgtatcc cgatgcggtg ttccagattg cacagttgct cgaagccgaa
     1441 aattcgggtg atattctcat caatgcggcc cccggttacg aaccctggca cgaaggtcag
     1501 aagggcgtgc atggcggtat tgatgccgct caactcaagg tgccgctggt actgtacgga
     1561 cgcggaatca ccccggggcg tctgccctgc gctcgcacgg tcgacgtgta cgtgacgatt
     1621 ctcaacctgc tcgacatcga gcacccacgt ggattgctgg gagttccgct gctggagcag
     1681 tgatggtggt gagggtcttc ggccgcgctg cggtcctggg acagccgcga ggatcattca
     1741 cgtgaagcat ggctggaagt tggctgtcgg agctgcattt gtcgtggctg gcctgtgggt
     1801 cgccggcaac tggttcgtca attctgctct gcctgttggc atactgagtg ctcagcactg
     1861 gcatgaaagc ctgcaaagcc gactggaacg agatcggtcg ggcctcgaga cactgaacca
     1921 tgaacagctt actccgctgc agacagcggt gaaaagcggc tgtattacgt gtgcaacctt
     1981 actgctgcgg gcgggggctg accccgaaaa gcctctcgcc ggccttctga atcttggtgt
     2041 gcgaccgctg catcttgcgg ccactgccgg agatccggag atggtcaagc ttctggcctc
     2101 ttccggtgca ccattgaacg cgacggatag cgtgggagac accgctctgc acgcggctgt
     2161 tcactggaag aaggaaggag ccgcgaaaac cctgctcgaa ctcggcgctg acccgaatgt
     2221 ggcagatgcc aacagctggg ggccattgca ctgggcggct ttgtggggcc atgacacgtt
     2281 ggtgccagcg ctcgttcaag ctggggcccg tatcgaccaa cccgttcgtg tcaagtatct
     2341 gctcgaagag ttcgtgcagc cacaacgcga tagaatggtc ggctatctcc atctgaccat
     2401 gtggcctgat ctcgaaggaa aattgccggc gcgccaacag aaataccccc tattttactg
     2461 gcacaacagg ccaaaggaaa agctgggagc ggaacaggcc gaagttgatc cgagtccacg
     2521 tttgatgaca ccgctcgatc tctcgcgtct gctcaaccgg gagtctgtcg ccgcgtatct
     2581 cgaatcacag cttgcgaaga aagccccggc cagagaataa aaccgggtga gagagcagat
     2641 tcagctgaag ctgcactgct cacgtttcac gggaatctgt tacccccgga gccagatctt
     2701 ctcgaagaat gtgccttcgg ccggtcgctc ggggatgagg gggacgttga ccagaaaaaa
     2761 agaaaccgac ggctcgaaag ccgtcggttt cggtgattct tcaggggccg gtcagtcggt
     2821 gacaatgacc gccgtagctg catacttgcg gtattcggcc ggtgacagga tgatttcggg
     2881 gtattcgttg ctgacccagt attggcgacc atcgggggcc tcgcgccatt caccgtcgag
     2941 caggttcact tcgcggccgg cgatgtaggc gatcttcttc ttgccgcgca tcatctgttc
     3001 tgacatgccg ctcagcgcgg tcatcacgcc tttcagttca acacggtttt catgattgat
     3061 cttgaactgc acccggttca gcaggcgacg aatcatgccc tgactcaaca gattggcctt
     3121 cttggtcagc ttttcggttt cctgctgatc ggcaagaatc tgttcggtca gcttggattc
     3181 ttgttcgctc agcttcaact cttcggtaac ggcttctggt agaggctgta gcacggtcag
     3241 ctcataggtg gcgctgatcg acagcagttc gtcaaccggg ctggaaaacg ccggcagcgc
     3301 gatcaggctg aggaaaagca gtagcaccag agatttaact gtgttgttca tgtgcgtgct
     3361 ccttgagcgg atacacagaa gaatgcaaga ggcataccac cagtggggtg gggaaaaccc
     3421 gcttcgtcac agagatctcg tcttcagtct tgggagtatg ctaaaatacc ggcagccgcc
     3481 tgccgggtat tcggcagc
//
LOCUS       DAORHD010000411         3496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_29970_length_3496_cov_3.098227, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000411 DAORHD010000000
VERSION     DAORHD010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29970_length_3496_cov_3.098227"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKO06_09640"
     CDS             <1..891
                     /locus_tag="PKO06_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNV69949.1"
                     /translation="QKIPKWSETLKSLWDKNPHKLRLVVLGSSSLQIQTGLTESLAGR
                     FELTRVFHWTYSELKKAFKYDLDRFLVFGGYPGSVPFEADFDRWYAYLKDSIVEAVIG
                     KDILLNRKVANPALFRQAFEILCRYPAQEVSYTKLLGQLQDKGNTDFVKYYIELYAGA
                     FLVQPLGKYSAKGWLTRSSSPKILLSCPALYTMTEGPQVLNDPERRGRLFELAVGSQL
                     LQLPGELFYWREKNDEVDFVYKYQGKLFAIEVKSGRRKSAKGLGVFAKNFPDAISVIL
                     TPDNFPEFSEDPRKFLAALE"
     gene            complement(956..2743)
                     /locus_tag="PKO06_09645"
     CDS             complement(956..2743)
                     /locus_tag="PKO06_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV69950.1"
                     /translation="MTREVVWLWGFWRPYWRRMTWIVILTALKTALFLSYPIFFKWIV
                     DGIRGGLTPEGLRNYVLGLILTGVGSSAVYILLQANRAMMNFRMEQMMRDRVFGKVLE
                     KDLTCFRTYPTGDVVTRLLDDLSDEKLCWFACSGIFRCLEAVFVLSFMVLILVNMNYK
                     LTLVTFLPLALITLVFLAVERKLHRLYHALQESVSKVNTFLETCFSGIRVVKAYGSEP
                     WQKQAFGVTVDDRAEAEVTLAKLDAGIHQTYGLMNSVGILLVLWLGGRMVVNGTLSLG
                     DFVAFSTYIHMVIEPMVSIGFFFTRGKQALVAIHRLLELEDAPVEVVTPVPSVPVGPF
                     EELRLENVSFRYGPDLPLVLENVGFALRRGEVVGIAGEVGSGKSTLFQLLLRFTDPSV
                     LATETGDRGRVLVNGIDVRQLDLAALRRLFAWVPQEALLFTDTVRANVAFERSFSDEQ
                     IAAVATQAEIAGEIEGFPQKYAQEVGQRGLTLSGGQKQRVALARALGHPADILLLDDL
                     TAALDARTEEKLWANLLAPEQRRTLLMISHRWATLQRCDRVVVLDRGRVVEEGPPQTL
                     LQRKGHLWQLVQRETLQEELQKNGNGSAH"
     gene            complement(2740..>3496)
                     /locus_tag="PKO06_09650"
     CDS             complement(2740..>3496)
                     /locus_tag="PKO06_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV69951.1"
                     /translation="FAYKGEDWVLRDVSFTVRRGQKLGIVGDTGGGKTTVLNLPLRFY
                     DPQRGSILIDGIDIRRFSTAQLRRLMALVLQDVFIFPGTVRENITLGANDVSAEQARE
                     AARQVFADGFISQLPKGYEGELAERGENVSVGQKQLISFARALARNPEILILDEATSA
                     VDPKTESLIQEAMTRLLSNRTSIVIAHRLSTILDADQILVFRHGQIIERGTHAELVVG
                     GGHYSRLYRLQARDQKNGIEKQVVAATEAGVPA"
BASE COUNT          766 a         1048 c          989 g          693 t
ORIGIN      
        1 cagaagattc ccaagtggtc ggaaaccctc aagtcgctgt gggacaagaa tcctcacaaa
       61 cttcgactgg tcgtattagg ttcaagctcc ctgcaaattc agaccgggct caccgagagc
      121 cttgccgggc gctttgaact gacgagggtc ttccactgga cgtacagcga actgaaaaag
      181 gcgttcaagt atgacctcga ccgctttctg gtttttggcg ggtatccggg ctcggtgccg
      241 ttcgaagctg acttcgaccg ctggtatgcc tatctgaagg attccattgt ggaagccgtc
      301 atcggcaaag atatcctgct caatcgcaag gtggccaatc cggcgttgtt ccgtcaggca
      361 ttcgagattc tgtgccggta tccagcgcag gaagtcagtt acacgaaact gctaggccaa
      421 ctgcaggaca agggaaacac cgatttcgtc aagtattaca tcgagctcta tgctggggcg
      481 ttcctcgttc aaccattggg aaagtattct gcgaagggat ggctcacccg ttcctccagt
      541 cctaaaattc tcctgtcttg tccggcgctc tacaccatga cggaagggcc acaggtgctc
      601 aatgacccag agcggcgggg tcgtctcttt gaactggcgg tcgggtcaca actgctgcag
      661 ctgcccggcg agctctttta ttggcgagag aaaaacgacg aagtcgactt cgtctataag
      721 tatcagggaa agctcttcgc catcgaggtg aaatccggaa gacggaaatc ggccaaaggc
      781 ctcggggtct ttgcaaagaa tttccctgac gccatcagcg tcattctgac cccggacaat
      841 ttccccgagt tttcagaaga tccccggaag tttctcgcag cccttgagtg accgcattat
      901 actcacaatc gggcggtgaa gatgaacagc accgaagtct gctgacaaaa actctttagt
      961 gggcactccc gttgccgttt ttctgaagtt cttcctgcag ggtttcgcgt tgtacgagtt
     1021 gccagagatg gcccttccgt tgcagcaggg tttgcggggg tccttcttcg acgacgcggc
     1081 cgcggtcgag caccacgact cggtcgcaac gctgcaaagt tgcccagcgg tgcgagatca
     1141 tcagcagggt gcgacgctgt tccggcgcga gcaggttcgc ccagagcttt tcttcggtgc
     1201 gcgcatcgag cgcggcggtc aggtcgtcga gcagcaggat gtcggcaggg tgacccagcg
     1261 cgcgcgccag cgccacgcgc tgtttctggc cgcccgacag cgtgaggccg cgctgtccga
     1321 cttcctgggc atacttctgc ggaaaccctt cgatctcgcc ggcgatctcg gcctgcgtgg
     1381 ccaccgccgc gatctgctcg tcgctgaacg agcgctcgaa cgcgacgttc gctcgtacgg
     1441 tatcggtgaa cagtagggct tcctgcggga cccaggcgaa gagacggcgc aacgccgcca
     1501 ggtcgagttg tcgcacgtca ataccgttta ccagcacccg accgcgatct ccggtttccg
     1561 tggccaggac ggaaggatcg gtaaaccgca gcagcaactg gaacaacgtc gacttgccgc
     1621 tgccgacttc gcccgcaatg ccgaccacct caccccgacg cagagcgaag ccgacattct
     1681 cgagcaccaa tgggagatcc gggccatagc ggaatgagac gttctccagg cgcagttctt
     1741 cgaacggacc aaccggtaca ctcggaacgg gcgtcacaac ttcgaccggg gcatcctcga
     1801 gttcgaggag tcggtgaatc gcgaccagtg cttgtttgcc acgagtaaag aaaaagccga
     1861 tcgagaccat cggttcgatg accatgtgga tataggtcga aaacgccaca aagtcgccga
     1921 gcgagagcgt gccgttgacg accatacggc ccccgagcca gagaaccagc agaatgccga
     1981 cgctgttcat cagaccgtag gtctggtgaa tgccggcatc gagtttggcc agcgtcactt
     2041 cggcctcggc ccgatcatcg accgtcacgc cgaaggcttg cttctgccag ggctcggagc
     2101 cgtaggcctt gacaacgcga atacccgaaa agcaggtttc gaggaaggtg ttgactttgg
     2161 aaaccgactc ctgcagggca tgatacagtc ggtgcagctt gcgttcgacg gccagaaaca
     2221 ccagggtgat cagggccagc ggcagaaagg tcaccaaggt cagcttgtaa ttcatgttca
     2281 ccagaatcag taccatgaac gaaagcacaa aaaccgcttc gagacagcga aagatgccgc
     2341 tgcaggcaaa ccagcacagt ttttcgtccg agagatcgtc aagcagacgc gtcacgacat
     2401 ctccggtcgg atacgtacga aagcaggtca gatctttttc gagcactttg ccgaacaccc
     2461 ggtcgcgcat catctgttcc atgcggaagt tcatcatggc gcgattggcc tgcagcagaa
     2521 tgtacaccgc cgaagagccg acgccggtca gaatcagccc cagcacatag ttacgcagcc
     2581 cttcgggagt caacccgcct ctgatgccgt cgacgatcca cttgaagaag atcgggtagg
     2641 aaagaaacag cgcggtcttc aaagcggtca ggatgacgat ccaggtcata cgtcgccagt
     2701 acggacgcca gaacccccac agccagacga cttcacgggt catgccggca cccccgcttc
     2761 cgtcgctgcc acaacctgct tctcgattcc gttcttttga tcacgtgcct gcaaccggta
     2821 cagtctggaa tagtgtccgc cgccgacaac cagctcggcg tgcgtgcctc gttcgatgat
     2881 ctgcccgtgt cgaaacacca gaatctggtc ggcatcgaga atcgtcgaga gtcgatgcgc
     2941 aatcacgatc gacgtgcgat tcgacagcaa gcgggtcatc gcctcctgaa tcagcgactc
     3001 ggtcttcgga tcgacagccg acgtcgcctc gtcgagaatc agtatctcgg ggttgcgtgc
     3061 caacgcccgc gcaaacgaga tcaactgctt ctgtcccacc gagacgttct cgccgcgttc
     3121 ggccagttcc ccttcgtatc ccttcggcaa ctgagaaatg aagccgtcgg cgaacacctg
     3181 acgagcggct tctcgggcct gttcggcaga aacatcgttc gcaccgaggg tgatgttctc
     3241 gcgtaccgtt cccgggaaaa tgaagacatc ctgcagcacc agagccatca ggcggcgcag
     3301 ttgggccgtc gagaaccggc ggatgtcgat gccgtcgatc agaatgctgc cacgctgtgg
     3361 atcgtagaat cgcagaggca gattcagcac cgtcgttttt ccgccccccg tgtcgccgac
     3421 gattcccagt ttctgcccac gtctgacggt gaacgacaca tcgcgcagca cccagtcttc
     3481 gcctttataa gcaaac
//
LOCUS       DAORHD010000412         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30014_length_3492_cov_4.230143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000412 DAORHD010000000
VERSION     DAORHD010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30014_length_3492_cov_4.230143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="PKO06_09655"
     CDS             complement(<1..2040)
                     /locus_tag="PKO06_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69952.1"
                     /translation="MPKSVAGWPVLLESVTRLRGAGRFPLPAYSEFMPPPRLGVKPTG
                     QIDPTVDMVNDPLGWPVSEWEEELELKPGLTFVARQIIDDLRRLYQGQRPPNLAGPQD
                     QNLRDNPAWPKNLSERTPSLTHERFSLLIPVALSRTQDDKGRIRWTLFGSSELGPEYA
                     FWKSFFQDDNPDKAASSFCTWTRAFLNRAYGLSLRSDEDLLASGFAVLPSESDPDFPD
                     WVRCSMPAALDKRLVLSAQTNASTSGLRYLLTFTPFQRLPESIQTAYLTGTLHLLPCP
                     ASLIFWYSSPFRRLQSHLPGALQIPLLRLVPRQEAMKGLRVPQSGWLREPRGTQELPA
                     LAEPLTKTTYARTNRWNRFQRYQSVLETSTLEEKVSQVFFSTDLQSLGLYGKPMARNA
                     QLWSLAGERILHGPIATPEELAQATLTIDAGGIFGYRFYYPPMRAGALEVTWHRPLVA
                     WSAEPGQVECQLADAPTGILAASTQGTSRSPATELMPRLLRRPEHLAAIREFARTLPS
                     DYYLWQTLFNAYKLLHTRQLLDRPLPKKLAQQLLSLNKRESFDDWARELPGNAFDTSA
                     GHELQKALSEVVIDDLADNRPEPLTFTRSATRAFEESYWKTIAFLAHGSFKTMNNADP
                     SLGATPIDSPPGKGTRDLDRIGDWLLQEYQRLIRHHELEGVVQAGELPFRWETDFP"
     gene            complement(2040..>3492)
                     /locus_tag="PKO06_09660"
     CDS             complement(2040..>3492)
                     /locus_tag="PKO06_09660"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV69953.1"
                     /translation="MMMQQTLRQYNGAVATPEEKIEIRVSINTGEVQVQDSDILGEPV
                     NVASRLEGITEVNEVWFTESTYLAMNKQEVPTSFIGEFRFKGVPEAQKIYRAIIDPDS
                     EFFKKMLTAQSQRSIPTESAAAGQAGSSVSINIGSTALLILVTLIVLGVGAWQAQRWS
                     RVKTLLQTIPAQTQQAQLLAARQSLEELLRLDPANEQFVRLGSDWLLSRIRGELKAQG
                     VSSATQALSEAYALFPRLQPLPLAEAEIKIAEALKLIEDGKKYEAMKIVDTLESDHAK
                     NFDIVRLVAGFYDRTGVHWYRGIVTMEAAYRLAPDKMASDPAFLEWVTYFLRQVSTDD
                     DVPTFRQLIRERFLPQYRSMLETSLYDPDPEKATLRHNARDFLTNASPPVTIDLFRFN
                     VCDLAVQWPNLNAPETAAVSDFFLNSEFQFPKATEATDRAWISSLTVQLEDVRKKAEE
                     AYGEDARRNGENATATRRLDALRKALNVLQSRL"
BASE COUNT          696 a          945 c         1130 g          721 t
ORIGIN      
        1 ggggaaatcg gtctcccagc ggaacggcag ttcgcccgcc tggacaacac cttcgagctc
       61 gtggtgccgt atcagacgct ggtattcctg cagcagccag tcgccgatgc ggtcgaggtc
      121 gcgcgttcct tttccgggag ggctatcgat gggagttgct cccaacgatg gatcggcatt
      181 gttcatcgtt ttgaatgagc catgggccag aaaggcgatg gttttccaat aggactcttc
      241 gaatgcgcgc gtggctgatc tcgtgaacgt gagaggttcg gggcggttgt ccgcgaggtc
      301 atcaatgacg acctcgctca acgctttctg cagctcgtgg ccggcggacg tgtcgaaggc
      361 gttgccgggc agctcgcgcg cccaatcgtc gaatgattct cgcttgttaa gagatagcag
      421 ttgctgtgcc agttttttcg gcagcggtcg gtcgagcaac tgtctggtgt gcagcagttt
      481 gtaggcattg aagagtgtct gccagagata gtagtcggat ggcagcgtgc gcgcgaactc
      541 ccgaatggcg gccaggtgct cggggcggcg cagcagtcgc ggcatgagtt cagtggcagg
      601 tgatcgcgaa gtgccttgcg tggatgcagc cagaatgccg gtgggagcgt cagcgagctg
      661 gcactcgacc tgacctggtt cagcggacca ggcaacgaga ggccgatgcc aggtgacttc
      721 gagggctccg gcacgcatgg gcgggtagta gaagcgatac ccgaagatgc cgccggcgtc
      781 gatggtgagc gtagcctgag cgagttcttc gggagtggcg atggggccat gaagaatgcg
      841 ctcgccagcc agactccaaa gctgagcgtt tctggccatg ggcttgccat acaggccgag
      901 cgactgcagg tcggtactga aaaagacctg cgacaccttt tcctcgagcg tcgaggtctc
      961 gagaacgctc tggtagcgct gaaatcgatt ccaccgattc gtgcgggcgt aggtggtctt
     1021 cgtgagaggt tcggccagtg ccggaagttc ctgggtgccg cgcggctctc gcagccatcc
     1081 cgactgggga acgcgcaggc ctttcatggc ttcctggcgg ggaaccaacc gcagcagggg
     1141 aatctgcaga gctccaggca ggtgcgactg caggcgccgg aatgggctgg aataccagaa
     1201 gatcagcgaa gcggggcagg ggagcaggtg gagtgtccca gtcagatagg cggtttgtat
     1261 cgattcggga aggcgttgaa agggagtaaa agtcagcaaa tatctgaggc cgctggtaga
     1321 tgcgttcgtc tgcgcactca acacgagacg tttgtccaga gccgcaggca ttgagcaacg
     1381 cacccaatct ggaaagtcgg ggtctgattc ggagggaaga acagcaaagc ctgaagccag
     1441 caggtcttcg tctgagcgca gcgacaaccc gtaggcacga ttcagaaacg cgcgtgtcca
     1501 ggtgcagaac gatgaagccg ctttgtccgg gttgtcgtcc tggaaaaagc tcttccagaa
     1561 agcatattct ggcccgagtt cgctggaacc gaacagcgtc cagcggatac ggcccttgtc
     1621 atcctgggtg cgcgacaagg ccacggggat gagcagcgag aaacgttcgt gtgtcaaaga
     1681 cggtgtgcgt tcagaaaggt tcttcggcca ggcggggttg tcacgcaggt tctgatcctg
     1741 cggccccgcg agattcggcg ggcgttgccc ctgatacaga cgacgcagat catcgatgat
     1801 ctgccgcgcg acaaacgtca gaccgggttt gagttcgagt tcctcttccc actcgctcac
     1861 gggccacccg agcgggtcat tcaccatgtc aacggtcggg tcgatttgcc cggtgggttt
     1921 cacgccgagc cgcgggggcg gcatgaactc tgagtacgcg ggcagcggaa aacgacccgc
     1981 gccgcgaagt cgcgtgacac tctcaagaag cactggccag ccggcaacag atttcggcat
     2041 cagagtctgg attggagcac gttcagggct ttgcgcaggg catcgagacg cctcgtggcc
     2101 gtggcatttt cgccattccg gcgtgcatct tccccatacg cttcctcggc cttcttgcgc
     2161 acatcttcga gttggacggt caggctcgag atccaggcgc gatcggtcgc ctccgtggcc
     2221 ttgggaaact gaaactcaga gttgagaaag aaatcggaga cggctgccgt ttccggggcg
     2281 ttgagattgg gccactgaac cgccagatca cagacgttga aacggaacag gtcaatcgtc
     2341 accgggggag aggcgttggt gaggaaatcg cgtgcgttgt ggcggagcgt ggctttctcc
     2401 ggatctgggt cgtagagcga ggtttcgagc atggaccgat actgcggcag gaagcgttca
     2461 cgaatcagct ggcgaaacgt cggcacgtcg tcatcggtgc tgacctggcg tagaaaatag
     2521 gtgacccact ccagaaatgc aggatcagaa gccattttgt cgggagccag gcggtaggcg
     2581 gcttccatcg tcacgatgcc gcgataccaa tgaacgccgg tgcggtcata aaatccggcg
     2641 accagccgca cgatatcgaa gtttttcgcg tgatcacttt caagtgtatc aacgatcttc
     2701 atcgcctcgt acttcttgcc gtcctcgatc agcttcaggg cttcggcaat cttgatttcg
     2761 gcttcggcga gcgggagagg ctgcaaccgg ggaaagagcg cgtaggcttc gctcagcgct
     2821 tgtgtcgccg aacttacccc ctgggccttc agttcgccgc ggatcctgga gagcagccag
     2881 tcagagccaa gacggacgaa ctgttcgttg gccggatcga gccgcagcag ttcttcaaga
     2941 ctctgccgag cggccagtaa ctgtgcctgc tgcgtctgcg cgggaatggt ctgcaacaga
     3001 gttttcacgc gtgaccaccg ttgtgcctgc cacgcaccca cgccgagaac gatcagcgtg
     3061 acgaggatca gtagagccgt cgagccgatg ttgatcgaga ccgatgatcc ggcttgacca
     3121 gccgccgccg attccgtcgg tatcgatcgc tgagactgag cggtgagcat tttcttgaag
     3181 aactcggagt cggggtcaat gatcgcgcga tagatctttt gcgcctctgg aactcccttg
     3241 aaacggaact ctccgatgaa gctggttggt acttcctgtt tgttcatggc gagataagtc
     3301 gattccgtga accagacttc gttgacttcg gtaatacctt ccagtcgtga ggcgacgttc
     3361 accggctcac cgagaatatc gctgtcctgc acctgaactt cgccggtgtt gattgagacg
     3421 cgaatttcga tcttctcttc gggggtggcg accgctccgt tatactgccg tagcgtctgc
     3481 tgcatcatca tg
//
LOCUS       DAORHD010000413         3491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30025_length_3491_cov_3.596333, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000413 DAORHD010000000
VERSION     DAORHD010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30025_length_3491_cov_3.596333"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="PKO06_09665"
     CDS             complement(<1..1582)
                     /locus_tag="PKO06_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV69954.1"
                     /translation="MPLELAALKPYSKSIVEATSAEEEFYYSPVVRCAPLPSPDAVVA
                     PVAQPVASSTPPTGEGEGTEGAPTEGGDEAAPTEGSDEAPADESGETQETDNLGDRVL
                     FIGFPVRTEGKSIGMALVGVNLEYVFESVTRVMEALEGKSAIVTGDPLQNGEYLAHPD
                     ESVVMDAQKNIFRDSTMIELMANAEEIMNSGGYSKFLTGKQVNVFLSSTKLAHAPWKL
                     MASVDTQSTLNALDKAQNFSLILIVVGLGLMTVVTLAVIRGVVKSIQVVSTGLEEISR
                     GRGDLTRRLAIVGRDELAEVSERFNKFIAYIQRLLLQVNDVINRLSGMSQQISGATSH
                     ENESVQKILGNTQRITKAEKQSALTLEKTAAAIEEISATAQLVAKRSNRAFEESVQNR
                     MKATQGMESVRDASSTIKGIERAVGDSSRVLEELKAQSRRIGKIVLTITAISRQTNLL
                     ALNAAIEAARAGDQGKGFVVVAENVKKLAEQSAKAAEEIGNLIGEIQHKTNKAVDEMN
                     QGKEKVQEGVSIINRAGAL"
     gene            complement(1787..2008)
                     /locus_tag="PKO06_09670"
     CDS             complement(1787..2008)
                     /locus_tag="PKO06_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69955.1"
                     /translation="MNFLANLRIKTRLFITIVCVSLFMLILSLFSSSKIRETVQSQVP
                     LQVKSLVSVEQRRLEQFLGMAKGFAKFFA"
     gene            complement(2031..>2838)
                     /locus_tag="PKO06_09675"
     CDS             complement(2031..>2838)
                     /locus_tag="PKO06_09675"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV69956.1"
                     /translation="KAILTRTGDYIIPLDRRSQIAENLRADAFVSIHVNSNRKRAVTG
                     IEVYCESPKGAVGEAERLVAETENQQDYIGAITGPGAGTSRKTQIVQKQAATMFQSQQ
                     LAHRVENRMQTAVPGLSSRGVKRAGFKVLHSLNLPSILVELGYISNAAEANLLKIQNN
                     QIRLAQSIYLGVRDFLENPPVQGYDSSYIAYVQAVEAEKRARAERIRKAQERRARMLA
                     NSIKYQVKKGETVAQIAAKFKVNQSDLRELNNLSKKSKLKAGQTLRVPRG"
     assembly_gap    2839..2848
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2849..3465)
                     /locus_tag="PKO06_09680"
     CDS             complement(<2849..3465)
                     /locus_tag="PKO06_09680"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV69957.1"
                     /translation="MIQFNHRYSLTLRTICALLLVLLPQLLGAQEQRITDIRFWQSPE
                     EAQVVVDMTEFPKVSPMETLPDGTFFFEIDTCKFRPGRASYPLNNQFLNAITVQERTT
                     GTVRIYFRVPAGIQYRTFLLPKNQVKPDRIVIFLSEPTQVVEMRRQEEIQEVTRLRAD
                     GQVKVVVIDAGHGGEDPGARHNGVIEKNYVLYMAKLVKSVFDKDPR"
BASE COUNT          679 a         1036 c          983 g          783 t
ORIGIN      
        1 gcagtgcacc ggcgcgattg atgatgctga cgccttcctg caccttctct ttgccctggt
       61 tcatctcgtc gacggccttg ttcgtcttgt gctgaatctc gccgatcagg ttgccgatct
      121 cttcagcggc cttggcactc tgctcggcca acttcttcac gttctcggcc acaaccacga
      181 agcccttgcc ctgatcgccg gcacgagctg cttcaatagc ggcattcagt gccagcaggt
      241 tcgtctgacg gctgatggcg gtaatcgtca gaacgatctt gccgatgcgc cgtgactgag
      301 ctttcaattc ttcaagcacg cgggaggagt caccgaccgc acgctcgatg cccttgatcg
      361 ttgacgaggc atcgcgcacc gattccatgc cctgcgtcgc tttcatgcgg ttctgcaccg
      421 attcttcaaa agcgcggttc gagcgtttcg cgaccaactg cgcagtcgct gagatctctt
      481 caatggccgc ggcggttttc tcgagcgtca aagccgactg cttttcggcc ttggtgatgc
      541 gctgcgtatt gccgagaatc ttttgtaccg attcgttttc gtgcgaagtc gcaccagata
      601 tctgttgcga cataccactc aatcgattga tgacgtcgtt cacctgcagc agcaagcgct
      661 gaatgtaggc gatgaacttg ttaaaccgtt ctgaaacctc ggccagttca tcgcgtccga
      721 caatcgccag acgccgggtc aggtcaccgc gcccgcgcga gatctcttcg agaccggtcg
      781 aaacgacttg aatcgacttg acgacgccgc ggatgacggc cagggtcacg acggtcatca
      841 gcccgagacc gacaacgatc aggatcagcg aaaaattctg cgccttgtcg agcgcattca
      901 gcgtgctctg ggtatcgacg ctggccatca gcttccaggg ggcgtgcgcc agtttggtcg
      961 acgagagaaa cacgttcacc tgtttgcccg tcagaaactt cgaataaccg cctgagttca
     1021 tgatttcttc ggcgttggcc atgagctcga tcatcgtgct gtcacggaaa atgttcttct
     1081 gggcatccat gacgaccgat tcatcggggt gggccagata ctcgccgttc tgcaacggat
     1141 cgccggtgac aatggccgac tttccttcca gggcttccat gacgcgcgtc accgactcga
     1201 acacgtattc gaggttcact ccgaccagag ccatgccgat gctcttcccc tcggttcgca
     1261 cgggaaaccc aataaacagc actcggtcgc cgaggttgtc ggtctcttgc gtttcgccgc
     1321 tctcgtcagc aggcgcttcg tcactcccct cggtgggggc ggcttcatcg ccgccttccg
     1381 tcggtgcgcc ttcggtgcct tcgccttctc cggtcggagg cgtcgaggac gcaaccggct
     1441 gagccacggg agcaaccacg gcatcgggtg acggaagcgg ggcgcatcga accaccggcg
     1501 aatagtagaa ctcttcttcg gcactcgtcg cttcgacgat cgacttgctg tagggcttga
     1561 gcgcagcaag ctcgagaggc atcgtcgccc cattcccgga atagcggaag cggacttcgc
     1621 ctttccgatc ggtcagaatg aactcgtgca ggaaacggtc gcgcccgtcg aatgagacgg
     1681 agagactctt cagaggcgat tcaatggcgc ttctccagag ttcgatgctg tcgagcgcgt
     1741 cgggtttctg gatcgtccag gagagaaact gaaccgacgc ttcgagtcag gcgaagaatt
     1801 tggcgaaccc tttggccatg cccagaaact gttcgaggcg tcgctgttcg acggaaacca
     1861 gcgacttgac ctgcagcggc acctgagact gaaccgtctc gcggatcttc gacgaactga
     1921 acaggctgag gatgagcatg aacagcgaaa cgcagacaat cgtaatgaac agacgcgtct
     1981 tgatgcgcag attcgccaga aagttcacgt gctcccccgt gtggccagct tcaaccccga
     2041 ggcactcgca gagtctgccc cgccttgagt ttgctcttct tgctcagatt attgagttcg
     2101 cgcagatcgc tctgatttac cttgaacttc gcggcaatct gcgcaacggt ctcacccttc
     2161 ttcacctggt atttgatgct gttcgcgagc atgcgcgcgc gccgttcctg ggctttgcga
     2221 atgcgttccg cacgggcgcg cttctcggcc tcgaccgcct gcacataggc gatataggac
     2281 gaatcataac cctgcaccgg cggattttcg agaaaatcgc gaactccgag ataaatcgac
     2341 tgggccagtc gtatctgatt gttctggatt ttcaacaggt tcgcttcggc cgcattcgag
     2401 atgtagccga gctcgaccag aatggaaggc aggttcaggg aatgcagcac cttgaacccc
     2461 gcccgcttga ccccacgtga cgagagcccc gggacggcag tctgcatgcg gttctcgacc
     2521 cgatgcgcca gctgctgact ctggaacatg gttgccgcct gcttctgcac gatctgcgtc
     2581 ttgcgcgacg tgcccgcccc cggcccggta atcgcgccga tgtagtcctg ttggttctcg
     2641 gtttcggcga ccagtcgctc ggcctcgccg acggcaccct tgggagactc gcagtagacc
     2701 tcgattcccg tgaccgctcg tttgcgattc gagttcacgt gaatgctcac gaacgcatcg
     2761 gctctcaggt tttcggcaat ctgagaacga cgatcgagag gaatgatgta gtcacccgtg
     2821 cgggtcagta tcgctttgnn nnnnnnnncg ggggtctttg tcgaaaacgc tcttcacgag
     2881 cttcgccatg tagagcacat agttcttctc gatgacaccg ttgtgacgtg cgcccgggtc
     2941 ttcgccccca tggccggcat cgatgacgac cactttgacc tgcccatcgg cccgcaggcg
     3001 ggtaacttcc tgaatttcct cctggcgacg catctcgacg acctgtgtcg gctcgctcag
     3061 aaagatcacg atgcgatcag gtttgacctg atttttcggc agcagaaacg tgcgatactg
     3121 gattccggcc ggtacgcgga agtagatgcg aaccgttccg gtggtgcgtt cctgcaccgt
     3181 gatggcattc agaaactggt tgttgagcgg ataacttgcc cgacccggtc gaaacttaca
     3241 ggtatcaatt tcaaaaaaga aggtgccgtc aggcagggtt tccataggtg aaaccttggg
     3301 aaactcggtc atgtcaacga cgacctgcgc ttcttcaggg ctctgccaga agcggatatc
     3361 cgtaatgcgc tgttcctgcg cacccagcaa ctgaggcagc aggacaagca gcagggcgca
     3421 gatcgttcgc agcgtcaggg agtatcgatg gttgaattga atcattgggg tgcacccgaa
     3481 agttctgaaa t
//
LOCUS       DAORHD010000414         3489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30053_length_3489_cov_3.885847, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000414 DAORHD010000000
VERSION     DAORHD010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30053_length_3489_cov_3.885847"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKO06_09685"
     CDS             complement(<1..315)
                     /locus_tag="PKO06_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HNV69958.1"
                     /translation="MEKIEQLREFAFISQLTRNLRRNPDQVNKCHEADAELIRQTDGS
                     FLAATVDTLYEEYHLGLIRDPRTLGWTVIMQSLSDLAAVAADPIGVLLSVTLPRDVHP
                     DWA"
     gene            complement(323..2692)
                     /locus_tag="PKO06_09690"
     CDS             complement(323..2692)
                     /locus_tag="PKO06_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV69959.1"
                     /translation="MQSSDVQSPGIRRGSLFFRPVAGLIDLTVRRPRVTLAIAAVLLF
                     LVGSGLLRLEVDFNVESFFPRDDGYRVSFQRYLNTFGRDDDLIILLCQPKLGSTSALL
                     PLRDLAAELRSHQAFQQVYSLFDVPVPQLIDGEEFRLPRLEHLLLASSTIDVERFSTL
                     TASFPAHSPIPHNFYDPASETFILLLRLSAERADNRGRIEVVTWLRHWLSQSRFTDSF
                     TCTLGGMPVARADGMILIEQDQRRLLPLALLVNLLVLWRLFGSVINVILVMVHVLLTL
                     AVTLGCMGHLDWRFSILSSVVPVILIITSSSYSIHLLSRWRHAQAQQEPPTISCVFAA
                     MFEPVGLATLTTLVGFLSLANANMQLITEFAYLTCLGVIVAFSLSFLLFPALVKVFPS
                     PTDRRTSAAPAPRESHSRLLSRALTWITRFNRRRPRVILILLLAAGIVSIAVGTGVRV
                     RAYVFDDFSSDSSFMRDIRQTEAVCDGILPMAVTIEPRGTETALRLSYLRQAATIARY
                     LRHQPEVGKVDSPSDMLSDLYAMLLEQPQNDGTLLPDSDSELGHLIRLLLAQGTVDPG
                     IRLVSRDLSALQVQFRLHDLESTKAAAFIDRLRDFLATVEQSNSRCALNGTTLMIQEA
                     YRHILSNLTIGFLLVIGFTLLVFLVTFRHTATIAAAFFGNLFPVCLILSLMALREIPF
                     KPSTITVFGIALGLAVDNTIYFLRECWRYLALGFSPGFASRHGLQRAGPGMIISSLTL
                     GSGFLVLLFSDFEAQFLIGLLLSFSVATALIYDLFFVPSAMILFGPKRV"
     gene            complement(2702..>3489)
                     /locus_tag="PKO06_09695"
     CDS             complement(2702..>3489)
                     /locus_tag="PKO06_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69960.1"
                     /translation="GPMTNLVSIIGNGVIAVLHKLATDEHKNLFKPTWVGYHTPKDKR
                     VSNWFCLIMGNHLKTYLTRVDQRSKLDEIGADPLQLNILDKNLFNFEVVKDLGNRATL
                     RVTPKRHANSNVVEAMIDLAQFKHGSAETWYATKITGKLTTGATGVTEFGNFRVAIAP
                     AGSYVAYSFDNSTRGPVPVMDIASALQAPAPKGDEKAFIFATQLKNTSYQSSRDVNEY
                     EIKLITTVQRLHLNPSTEAMAQYIQGKRLGTLEKVAKKVVSSH"
BASE COUNT          815 a          892 c         1140 g          642 t
ORIGIN      
        1 ggcccagtcg gggtgcacgt cgcggggcag ggtgaccgac aacagcacgc cgatcggatc
       61 agcggcaacg gcggccagat cgctgagact ctgcatgatg acggtccagc cgagcgtgcg
      121 cgggtcgcgg atcagaccaa ggtgatactc ttcatacaat gtatcgaccg tggcggcgag
      181 aaacgaacca tccgtttgac gaatcagctc ggcatctgct tcgtggcact tgttgacctg
      241 gtcgggattg cggcggagat tccgcgtgag ttggctgatg aaggcgaatt cgcgaagctg
      301 ctcaatcttt tccatacgga gattataccc gttttggacc aaaaagaatc atggctgacg
      361 gaacgaagaa cagatcatag atcagagccg tggcgacgct gaaactgagc agcagcccaa
      421 tgagaaactg tgcctcgaag tccgagaaca gcagcaccag aaaccctgaa cccagcgtca
      481 gggaagagat gatcattccc ggaccggctc gttgtagccc gtgacgactg gcaaatccgg
      541 gagagaatcc gagggccaga tatctccagc actcgcgcag aaagtagatg gtattatcga
      601 ccgcgaggcc aagagcgatt ccgaacacgg tgatggtcga aggtttgaac ggaatctcac
      661 gcagcgccat cagactcaga atcagacaga cgggaaacag gttcccgaag aaggctgccg
      721 caatggttgc ggtgtgacga aaggtgacga gaaaaaccag cagcgtgaag ccaatgacca
      781 gcagaaagcc gatggtcagg ttcgagagaa tgtgccgata ggcctcctga atcatcaggg
      841 tcgtgccatt gagtgcgcac cggctgttcg attgctcgac cgtcgcgaga aagtcacgca
      901 accgatcgat gaaagccgca gccttggttg attcgagatc gtgcaggcga aactgtacct
      961 gtagagcgct cagatcgcgg ctgaccagcc gtatccccgg gtctaccgtt ccctgtgcca
     1021 gcagcaatcg aatcaggtgc ccgagttcag agtccgagtc tgggaggagt gttccgtcgt
     1081 tctggggctg ttctaagagc atggcgtaga gatcgctcaa catgtcagac ggactgtcga
     1141 cttttccgac ctcaggctgg tggcgcagat acctggcgat ggtcgcggcc tgtcgcaggt
     1201 aagacagacg gagcgcggtt tctgttccgc ggggttcgat cgtgacagcc atgggtagaa
     1261 tgccatcgca cacggcttcc gtttgccgaa tgtcacgcat gaaggaggaa tcagaggaga
     1321 agtcatcgaa gacatacgca cgcacccgta cgcctgttcc cactgcgatt gaaacaatgc
     1381 ccgccgccag caggaggatc aggataaccc gtggacgtcg gcgattgaag cgggtgatcc
     1441 aggtcagcgc acgggacagg agacgagagt ggctctcacg tggtgcaggg gcggctgacg
     1501 tccgccggtc ggtcggactc ggaaagacct tgaccagagc gggaaagagg agaaaggaca
     1561 gactgaacgc gacgatgacg ccgagacacg tcagatacgc aaactcggtg atcagctgca
     1621 tgttggcatt tgccagcgag agaaatccga cgagggtcgt cagggtggcc aggcccacgg
     1681 gttcgaacat ggcggcaaag acgcaggaaa tcgttggcgg ttcctgctgt gcctgggcgt
     1741 gtcgccagcg tgacaagagg tgaatgctgt agctcgaaga cgtgatgatc agaatgacgg
     1801 ggacgaccga cgaaagaatg gaaaagcgcc agtcgagatg ccccatgcaa ccgagagtca
     1861 ccgccagtgt gagcagcacg tgaaccatca cgagaatgac gttgatgaca ctgccgaaca
     1921 gtctccagag taccagcagg ttgacgagca gggcgagggg gagcagacgc cgttgatcct
     1981 gttcgatgag gatcatgccg tcggcgcggg caacgggcat gccgcctaaa gtacaagtga
     2041 aagagtcggt aaaccggctt tgtgaaagcc agtgacgcag ccaggtgacg acttcgatgc
     2101 gaccccgatt gtcggcgcgc tccgccgaga gacgcagcag cagaatgaac gtttcggatg
     2161 ccgggtcgta gaagttgtgg ggaatcggag aatgggccgg gaaagaggcc gtgagggtgg
     2221 agaaccgctc gacatcgatg gtcgaggaag cgagaagcag gtgttccaga cgcgggagtc
     2281 gaaactcttc tccgtcgatg agttgaggca ccggcacatc gaaaaggctg tacacctgtt
     2341 gaaaagcttg atgggaacga agttcggcgg cgagatcgcg caggggcagc aacgcggatg
     2401 tcgaccccag ctttggctgg cacagcagaa tgatcagatc gtcgtcgcgc ccgaacgtgt
     2461 tcagatatcg ctgaaacgag acgcgatacc cgtcgtcgcg cgggaagaag ctttcgacgt
     2521 tgaaatcgac ttcgagtcgc agaagcccgg aacccacgag aaacagaagc accgccgcga
     2581 tggccagggt gactcgcggc cgtcgaacgg tcagatcgat cagacctgcg acgggacgaa
     2641 aaaacaacga gccccggcga atgccggggc tctgaacgtc agacgattgc acgaacagga
     2701 atcagtggct cgaaacgacc ttcttggcga ccttctcgag ggtgccgagg cgcttgccct
     2761 gaatgtactg ggccatggct tcggtggagg gattcagatg caggcgctgc acggtggtga
     2821 tgagcttgat ttcatattca ttcacatcgc gtgaactctg atagctggtg ttcttcagct
     2881 gagtcgcgaa gatgaatgcc ttttcgtcac ccttcggtgc cggggcctga agcgcgctgg
     2941 caatatccat gactggtact ggtccacggg tggagttgtc gaacgagtag gcaacgtagc
     3001 tgcccgccgg agcgatggcg acacggaagt tgccgaactc ggtcacgccg gttgcgccgg
     3061 tggtgagctt gccggtgatc ttggtggcat accaggtttc ggcggagccg tgtttgaact
     3121 gggccagatc gatcatcgct tcgacgacgt tggaattggc gtgccgcttg ggggtgacgc
     3181 gcagcgtggc gcgattgccc aggtctttca cgacttcgaa gttgaacaga ttcttgtcga
     3241 gaatgttcaa ctgcagcggg tcagcgccga tctcgtcgag cttggagcgc tggtcgacac
     3301 gggtcaggta ggttttcaga tggttgccca tgatcaggca gaaccagttg gaaacgcgct
     3361 tgtctttggg ggtgtgataa ccgacccagg tgggcttgaa gaggttcttg tgctcgtcgg
     3421 tggcgagctt gtgcaacacg gcgatgaccc cgtttccgat gatgctcacg agattcgtca
     3481 tcggaccat
//
LOCUS       DAORHD010000415         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30095_length_3486_cov_3.989799, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000415 DAORHD010000000
VERSION     DAORHD010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30095_length_3486_cov_3.989799"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="PKO06_09700"
     CDS             <1..1523
                     /locus_tag="PKO06_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV69961.1"
                     /translation="VRTELKPEQREYVETINSCGATLLALINDILDFSKIEAGRIELE
                     KVPFDPRYLFEETLGLVTDTAQSKGLELEFIVESLLPAALLGDPGRLRQVLLNLLSNA
                     IKFTPRGYVCLRVEAERPSGGGCRMTVDVSDSGIGISPEQSAKLFQPFSQADASTTRR
                     FGGTGLGLSICRRLSELMGGTIEIISELNQGTTFRLRVELPVVEEAPKVPEWIAGCRE
                     QRVLVFDGHEVTARATATQCRLWHLEPVFCPRHAELEAVLQSGGKDVKPWLAVVIHVP
                     SSGLEDVVEVVQTLREHPMGSRLPVIISIPGFRRAAAADFMKRAGGVCCLRRPFKQAD
                     VLGCLQTVMMGVEQKVVAEPAPKLESEKNTLGQTTWAEGRSVLLAEDNPVNQLVTSTI
                     LKKLGMQVRIAANGKEAVRLFQEATFDIILMDCQMPEMDGFTATRTIRERSGGLHTPI
                     IALTAGATEEDRLRALEAGMDDFLLKPIQPDHLTRTLKRWMEPGLSAPRPPTNHPE"
     gene            1520..2482
                     /locus_tag="PKO06_09705"
     CDS             1520..2482
                     /locus_tag="PKO06_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV69962.1"
                     /translation="MMATFHTKHEGGTRRRLCLSVLLIRCLLLLAIAQPVVVSAQDPK
                     LRQLNVGYSSVMISNVDLKDAQIALKVWSDMLERDKKGKWEQQATIYPTTRELIEAYR
                     AGKADIIAFTVLEYLLFKDQVEMTPELVGEISGSPCQQNLLLVNSKSGITSLEQLKGK
                     TLIIENQGTGNIPILWLDIILKKSGLERMRKHFAIVKEVQAPSKAATPVFFQQAHACI
                     VRESGFKTLIDLNPQYQRELTVLASSSAFLRGITCYRRDFDPADKQDLMSSAVKLHET
                     VRGQQILTLFKVDRILPFKPEMLDTAVQLIQEYQKLFPTVAIEP"
     gene            2807..>3486
                     /locus_tag="PKO06_09710"
     CDS             2807..>3486
                     /locus_tag="PKO06_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69963.1"
                     /translation="MKSPNTPAAPPPWGLTRKTALFSSLVLMIALALFAYLVLPLLRT
                     TLEKSLQSQGAALSASIAQVVTSSILAEEYSGVVDHCLEVLKRVPTVRYIVIARHDGF
                     CLLLQPESWTQETLGKIWAKPSETAIRGQFERNPFGTDLLYHQAHLLTYSGIEWGWLH
                     LGLSIGGYEADLHTLYKTLAQIALFSILVGLALAGYFAGRLVRPLRQLQTFAGQVAAG
                     DLTHGPIVR"
BASE COUNT          730 a          992 c         1024 g          740 t
ORIGIN      
        1 tggtgcgcac cgagctcaaa cccgaacaac gggagtatgt cgaaaccatc aattcctgcg
       61 gcgccacgtt gctggcgctg atcaacgaca ttctcgactt ctccaagatc gaggcgggtc
      121 gcatcgagct cgagaaggtg cccttcgacc cgcgatatct gttcgaagaa acgcttggat
      181 tggtcaccga taccgcgcag agtaaaggac tggaactgga attcatcgtg gaatctctgt
      241 tgccggccgc attgctgggc gacccggggc gtctccgcca ggtgttgctg aacctgctca
      301 gcaacgccat caagttcacc ccgcgaggct atgtttgctt gcgtgtcgag gccgagcgac
      361 catctggcgg agggtgtcgc atgacggtcg atgtcagcga ttccggcatt ggcatttctc
      421 ccgaacagtc ggcgaagctt tttcaacctt tctctcaggc tgatgcctca acgacgcggc
      481 gcttcggcgg aaccggactg ggactttcga tttgtcgtcg tttgtccgag ttgatggggg
      541 gaacgatcga aatcatcagc gagttgaatc agggaacgac gtttcgtctg cgcgtcgaat
      601 tgccggtggt cgaagaagcc ccgaaggtgc cggaatggat tgccggatgc cgggaacaac
      661 gggtgctggt cttcgatgga catgaggtca cggcgcgcgc gaccgcgacg cagtgccggt
      721 tgtggcacct cgaacccgtg ttctgtcccc gacacgccga gcttgaagcc gtgctgcagt
      781 ctggcggcaa ggacgtgaaa ccctggttgg cggtggttat ccatgtcccc tcgtccgggc
      841 ttgaagatgt cgttgaagtc gtgcagacct tgcgtgaaca tccgatgggg tctcgcctgc
      901 cggtcatcat ttcgataccc gggttccgtc gtgcggccgc ggcggacttc atgaagcggg
      961 cggggggagt ctgctgtctg cggcgcccct tcaaacaggc ggatgttctg ggttgtctgc
     1021 agaccgtgat gatgggagtc gagcaaaagg ttgttgccga acccgctccg aagcttgaat
     1081 ctgagaaaaa caccctggga caaacgacgt gggccgaggg gcgcagtgtt ctcctggccg
     1141 aagacaatcc cgtgaatcag ctggtcacat cgaccattct gaaaaagctt ggcatgcagg
     1201 tgcggattgc cgcaaacggg aaggaagcgg tgcgtctctt tcaggaagcg acattcgata
     1261 tcattctcat ggactgccag atgcccgaaa tggatggatt cacggccacc agaaccattc
     1321 gtgaacgttc aggggggctg catactccga tcatcgccct cacggccgga gcgaccgagg
     1381 aagatcgttt acgggcactg gaagcgggaa tggacgactt cctgctgaag ccgatacagc
     1441 ccgatcatct gacccgcacc ctgaagcgct ggatggaacc cggcttgtcg gctcccagac
     1501 cgccgacgaa ccacccggaa tgatggcgac gtttcacacg aaacacgaag gcggaactcg
     1561 acgccggctg tgcttgtcgg tgctgctgat ccgctgcctg ttgctgctgg caatcgcgca
     1621 gccggtcgtc gtctcagccc aggacccgaa actgcggcaa ctcaacgtcg gttactcgtc
     1681 ggtgatgatt tccaacgtcg atctgaaaga tgcgcagatt gccctcaaag tctggtcaga
     1741 catgcttgaa cgcgataaaa agggcaagtg ggaacagcag gcgacgattt atccgacgac
     1801 gcgggaactg atcgaggcgt atcgtgccgg gaaggccgat atcatcgcct tcacggttct
     1861 tgagtatctt ctcttcaaag atcaggtcga gatgaccccc gaactggtcg gggaaatctc
     1921 cggcagcccc tgtcagcaga acctcttgct cgtgaattcc aaatcaggga ttacgagtct
     1981 tgagcaattg aaaggcaaga ccttgatcat cgaaaaccag ggaacgggca acattcccat
     2041 tctctggctc gatatcatcc tgaaaaagtc cggtctggag cgaatgcgca aacacttcgc
     2101 catcgtgaaa gaggtgcagg ccccttccaa ggcggcaact ccggtgttct tccagcaggc
     2161 gcatgcctgc atcgttcgtg aatcgggttt caagacgttg atcgacttga accctcaata
     2221 tcagcgtgaa ctcacggttc tggcctcgtc ttcggccttt ctgcgcggaa tcacctgcta
     2281 ccgccgtgat ttcgatcccg cggacaagca agacctgatg tcgtccgcgg tgaaactgca
     2341 cgagacagtg cggggacagc agattctcac gctattcaag gttgatcgca tcctcccgtt
     2401 caaacccgaa atgctcgaca ctgctgttca gctcattcag gagtatcaaa agctgttccc
     2461 cactgtggca atcgaaccct gaagagatgt aacactgagc ggaagaccga gttctacagc
     2521 ttcgccaggc caaacgggaa cttttcgctg acgcggagtg tctggagcgg tctgacgaac
     2581 ggaagctcaa acgcccgtca cgcgaggaaa aagttcccgt ttgccctgtt tgccctgcgg
     2641 catcgctggc agggaattca acagagtgct agaatgcgaa accaccacgg tggatctcga
     2701 atcacttttc tgcaggggcg gtcgtctcac ttttcgttca cacatcatgg aaataacgca
     2761 gaccatcatc gttccgccgg gctctgaacc acgagcttct gaatcggtga aaagcccgaa
     2821 tacccccgct gccccgccac cctgggggtt gacgcgcaaa accgcgttgt tttcctcgct
     2881 cgtgctgatg attgccctgg ccctgtttgc ctatctggta ctgccgttgc tgcgcaccac
     2941 actggaaaag agtctgcagt cgcagggagc cgcgttatcg gcttccatcg cgcaggtcgt
     3001 gacctcgtcg attctcgcgg aagaatacag cggcgtcgtc gatcactgtc tcgaagtgtt
     3061 gaagcgggtg ccgaccgttc gttacattgt catcgccagg catgacggct tctgtctgtt
     3121 gctgcagccg gagtcctgga cgcaggaaac gttgggaaag atctgggcga aaccctctga
     3181 aaccgccatt cgcggccagt tcgaacgaaa cccgtttggt actgacctgt tgtaccacca
     3241 ggcacacctg ctcacctact cggggatcga atgggggtgg ctgcatctgg gtttgagcat
     3301 cggcgggtac gaggcagatc tgcacaccct ctacaaaacc ctggcgcaga tcgctctctt
     3361 ctcgattctg gtcggcctgg cgctggcggg atactttgcc ggacgcctgg tgcggcccct
     3421 gcgacagttg cagacgtttg ccggtcaggt ggctgccgga gatctgactc atggcccgat
     3481 agtacg
//
LOCUS       DAORHD010000416         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30113_length_3485_cov_3.925364, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000416 DAORHD010000000
VERSION     DAORHD010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30113_length_3485_cov_3.925364"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKO06_09715"
     CDS             <1..397
                     /locus_tag="PKO06_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69964.1"
                     /translation="AFDTASGSREIDIPETIRDRNLMIEVTNGSVTRTQPYYPNKLGV
                     QVQENYGQLRVTKAGTGTPLPRVYVKVYAQLSDGQVIFYKDGYTDLRGRFDYTSLSTS
                     ELDQTQKFSLLIVSDQFGAVVREAAPPKR"
     gene            complement(450..1226)
                     /locus_tag="PKO06_09720"
     CDS             complement(450..1226)
                     /locus_tag="PKO06_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HNV69965.1"
                     /translation="MERDFLRYDGTFPGLIRALGPWLKRRELPTVIERAGCRQPSLFG
                     DHLEMTAEASNDDLRLVPTAEDFAAVAMPEVTMTIWRRVWVAFLAEVTGIERQVGEYL
                     LLAVERGNDIEDLLTDPRVQRIQRCESRVRREVHAFKGLLRFQQIEERLFLAVITPET
                     CILPMIAPHFARRFADQAWIIHDRRRKLAAVCEQGTVTLVPQWAAEETLLQQTEQEMQ
                     ELWRCFFRQAAVRERLNLAAQQRHIPRRYWNDLIEEPGRE"
     gene            complement(1260..2585)
                     /locus_tag="PKO06_09725"
     CDS             complement(1260..2585)
                     /locus_tag="PKO06_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HNV69966.1"
                     /translation="MNTAAAPAPATPTLVQKLEILAASARYDASCVSSGSTRRAAKGG
                     VGNVAPSGICHSWAADGRCISLLKVLFTNHCIYDCAYCLNRRSNPIVRAMFTPEELAE
                     LTIGFYRRNYIEGLFLSSGVFRSPDTTMELLLRTVRLLRETHQFQGYIHLKAIPGADP
                     LLLFQAGQLVDRLSVNVELPSEASLSVLAPEKRKDSIFGPMRRIRDWHQEAHPAPGKR
                     ARQPYVPAGQSTQVIIGASPETDQQILKLSSGLYKTMGLRRVFYSAYVPVNRDQRLPE
                     LPIPPLLREHRLYQADWLMRFYQFGVDELFAGGAGNLDAELDPKVMWALHHLDLFPLD
                     VNRADYEFLLRVPGVGVRSAQRIVRARKTGRLTLDDLPKLGVVWKRARFFLGDGVLSP
                     GGWFDADRLRRELVKPTVPAHRQLTFADLPDAIDSSPVENLRAAVTGEF"
     gene            complement(2620..2910)
                     /locus_tag="PKO06_09730"
     CDS             complement(2620..2910)
                     /locus_tag="PKO06_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNV69967.1"
                     /translation="MTLSACGVKCRLCPHHQKQCSGCAQVKGSPWWAVEHVPGKVCPI
                     YACAVNTKKLAHCGDCARLPCQTFTELRDPSMSDQEYQEQLNLRLERLRSAK"
     gene            complement(3050..>3485)
                     /locus_tag="PKO06_09735"
     CDS             complement(3050..>3485)
                     /locus_tag="PKO06_09735"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69968.1"
                     /translation="LSERELKSKVEIKAETYNKTKEIEIKTAINLARTQFLPTLSRQV
                     TQSANAVAALKAAGVESKAMLEDLKRFEKRYQEIREGVDRLEKTLHKAETHHETLDRA
                     RFLSDEGEKSLLHLRAVVDAAEEYVAADLWPMAKYQELLTIL"
BASE COUNT          725 a         1111 c          963 g          686 t
ORIGIN      
        1 ggcatttgat acggccagcg ggagtcgcga gatcgacatt cccgaaacga ttcgcgatcg
       61 caacctgatg atcgaggtga cgaacggcag cgtgacccgt acgcaacctt actaccccaa
      121 caaactcggg gttcaggtgc aggaaaacta cggccagctg cgcgtgacaa aagccggaac
      181 cgggacgccg ctgcccagag tctacgtgaa ggtgtatgct caactgagcg acgggcaggt
      241 catcttctac aaagacggct acacggatct gcgcggccgt ttcgactaca cctcactgag
      301 caccagcgaa ctcgaccaga cccagaagtt ctcgctgctg atcgtcagtg accagttcgg
      361 cgccgtcgtg cgcgaagccg ctccgcccaa gcggtaagtc tcgttcccga accgggctga
      421 aaccatcagg tttcggcccg gtttcctttt cactcgcgcc cgggttcctc gatgaggtca
      481 ttccagtatc gtcgggggat gtgacgctgc tgggccgcga gattcaaccg ctcgcgcacg
      541 gctgcctgac ggaagaagca ccgccagagc tcctgcattt cctgctcggt ctgctgcagg
      601 agggtctctt cggccgccca ctgcgggacc agcgtgaccg tcccctgctc gcacacggcg
      661 gcgagcttgc ggcggcgatc gtggatgatc cacgcctgat cggcgaaccg ccgcgcgaaa
      721 tgcggcgcaa tcatcggcaa aatgcaggtt tcaggggtaa tcacggcaag aaacagccgt
      781 tcctcgatct gctgaaaccg cagcagcccc ttgaaggcgt gcacttcccg tcgcacccga
      841 ctctcgcagc gttgaatgcg ttgcacccgg ggatcggtga gcaggtcttc aatatcgttt
      901 ccccgctcta ccgccagcag gaggtattcg ccgacctgcc gctcgatgcc ggtcacttcg
      961 gccagaaacg ccacccagac gcgacgccag atcgtcattg tcacttccgg catcgctacc
     1021 gcggcaaaat cttctgctgt tggtacaagt cgcagatcgt cattacttgc ctcggcagtc
     1081 atttccagat gatcaccaaa cagcgacggt tgtcggcacc cagcccgttc aatcaccgtc
     1141 ggaagctcac ggcgtttcag ccatggcccc agcgccctga tcaggccagg aaacgttccg
     1201 tcatatcgca gaaagtcccg ttccatcttc accctcattc cccgctcaac gtctgaaatt
     1261 cagaactcac cggtaaccgc cgcccgcagg ttttccaccg gcgaggaatc gatcgcgtcg
     1321 gggagatccg cgaatgttaa ctgccgatga gccggcaccg tcggtttcac cagttcgcgt
     1381 cgcaagcggt ccgcatcaaa ccagccgccg gggctgagca ctccgtcacc gaggaaaaac
     1441 cgcgcccgtt tccacaccac ccccagcttc ggtagatcgt ccagggtgag ccgcccggtt
     1501 ttccgcgccc gaacaattcg ttgagccgaa cgaactccaa cgccgggaac acgcagcaag
     1561 aactcgtagt cggcgcgatt gacatcgagt gggaacaggt ccagatggtg caacgcccac
     1621 atgaccttcg gatcgagttc agcatcgagg tttcccgcac cgccggcaaa cagttcatcc
     1681 accccgaact gatagaaacg catcagccag tctgcctggt acaatcgatg ttcgcgcaaa
     1741 agcggcggaa tgggcagctc cggaagtcgc tggtctcgat tcacgggaac atacgccgaa
     1801 tagaacacgc gacgcaggcc catagtcttg tacaatccgg aagacagctt cagaatctgc
     1861 tggtcagttt caggagaagc gccaataatg acctgcgtgc tctggccggc cggcacgtac
     1921 ggttgtcgcg ctcgtttgcc cggcgccggg tgcgcttcct gatgccagtc gcgaatgcgc
     1981 cgcatcgggc cgaatatgga gtccttgcgt ttctcggggg ccagcacgct gagactcgcc
     2041 tcgctcggca gttcgacgtt cacgctgaga cgatcgacca gttgtccggc ctgaaacagc
     2101 agcaacggat ccgctccggg aatcgccttc aaatgtatgt acccctgaaa ctgatgggtc
     2161 tcgcgcagaa gtcgcaccgt acgcaacagg agttccatcg tggtgtccgg cgagcggaac
     2221 actcccgaac tgagaaacag accctcgatg tagttgcgac gatagaatcc gatagtcagc
     2281 tcggccagct cttcaggtgt gaacatggct cggacaatcg ggttgctgcg ccgattcagg
     2341 cagtaggcac agtcatagat acagtgatta gtgaacagta ctttcagcag tgaaatgcag
     2401 cgcccatcgg ccgcccacga atgacagata ccgcttgggg ccacatttcc caccccaccc
     2461 ttcgcggctc ggcgggtgct gccactcgaa acgcaggacg catcataccg cgcactggca
     2521 gccagaattt caagtttctg taccagtgtc ggtgtagcag gggcgggagc ggcagctgta
     2581 ttcatcttgc actgacccgg agccagacgg ctcaacgcct cacttggccg aacgcaacct
     2641 ttccagacgc agattcagct gctcctggta ttcctgatcc gacatcgatg gatcgcgcaa
     2701 ctcggtaaac gtttggcagg gcagtctggc acagtcgcca cagtgcgcca gtttcttcgt
     2761 gttgacggca caggcataga tcggacacac cttccccggc acgtgttcaa ccgcccacca
     2821 cggcgatccc ttcacctgcg cgcaacccga gcattgcttc tggtgatgcg gacacaaccg
     2881 gcacttcact ccacaggcac tcaacgtcat gagagtcttc cttactcttg cgaaatgcga
     2941 accatcccgt ggcagtctac cacgcatggc ggctctcagc gaacccgccg agaagagaac
     3001 acccccgctc gtgcgtcatc ggcacaagcg ggggtgaggc tcagagcaat cagagaatgg
     3061 tgagcagttc ctgatatttg gccatcggcc acagatcggc ggccacatac tcctcggccg
     3121 catcgacgac cgcacggaga tgcagcagtg atttttcgcc ttcgtcactg agaaaccgag
     3181 cccgatccag ggtctcgtgg tgcgtctcag ctttgtgcag cgtcttttcg agacggtcga
     3241 caccctcgcg aatctcctga tagcgtttct cgaaacgctt gaggtcttca agcatcgcct
     3301 ttgattcgac ccccgccgct ttcagtgctg cgaccgcatt tgccgactgc gtgacctgac
     3361 ggctcagggt tggcaggaac tgcgtgcgcg ccagattgat cgccgtcttg atctcgatct
     3421 ctttggtctt gttataggtt tccgccttga tttcaacctt cgacttcagt tcgcgttcgg
     3481 agagc
//
LOCUS       DAORHD010000417         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30115_length_3485_cov_3.481341, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000417 DAORHD010000000
VERSION     DAORHD010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30115_length_3485_cov_3.481341"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..995)
                     /locus_tag="PKO06_09740"
     CDS             complement(291..995)
                     /locus_tag="PKO06_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69969.1"
                     /translation="MKGIGVGCVVLVLLFMLTSAIEARETGQTIVDTQMLSTFAKVDL
                     KDGVSQDEARVLAGDYLLHFVSGCGGIAEIKDKGAAWEATPLVGRGATPGEVILVDKK
                     TGKLSSVRGPTIVAPVEQNSDELSTPPAELDAKIFELGEITSTTRDEELHQIKLGLTI
                     SYKGDETVLTQQTETIRDQVVQLLMKMTMEKAQEAFTTRTLHSDIEAIVRKAIAGAAA
                     QSFQLRDVQLVDFLVK"
     gene            complement(1056..1682)
                     /locus_tag="PKO06_09745"
     CDS             complement(1056..1682)
                     /locus_tag="PKO06_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV69970.1"
                     /translation="MLQRVEGKRVYLRPFTLEDTEKVFRMSIESGMKQWIPDQVYESV
                     AHAREVLTFLSEQYESPGGPADKPLVFGVCLKSTGELIGHVGLSPLKTDVEVGYAIEE
                     CQQGHGYAADAVHTMTAWAMEQYRLPLVLGVVPADNHASIRTLENSGFQFCREVRRRF
                     HGRLTQVRIYELASAAPTKSPRQTRGKSSVEKDKKLVIIGSTRKDEGR"
     gene            2351..3409
                     /locus_tag="PKO06_09750"
     CDS             2351..3409
                     /locus_tag="PKO06_09750"
                     /inference="COORDINATES: protein motif:HMM:NF021386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HNV69971.1"
                     /translation="MKVDELVAIGKGLADRARVRMLRSLLLTPQYLEELAKRLNLPLS
                     TTSFHVNKLVEAGLVCRRREQYYAVFEIPRDTLPSPLIELLANLDDSETGESTRVRKL
                     RDETIKRSFRNGVLLTIPTQKKKRLLVLEEIARRFDADRDYAEKDLSRLLERINPDYC
                     LLRRLLVDEGFMVRSHGVYRRVHRPPVLIENAVGPAPRSPAPPIPPSPANKRLRTPPS
                     TKDPRMTTDRKQLKREALEQKPEMGIFKIENRTNGKIFLVVAPNIPAYMTRHRFDLEL
                     GSHPNRELQADWKKHGPDAFTFEVLETIDQKDESRDHNLDELKSLGARYLKKLHPWDD
                     RGYHDRPRERQISARGED"
BASE COUNT          756 a         1123 c          869 g          737 t
ORIGIN      
        1 gacccgtccc ggctctcgga actcgtcgcg gtcgtggccg tttccacctc gccctcgctc
       61 gcggggggag cactgaaccc atgcgaacag atcgccatca ccacccagaa cgttaataac
      121 cagatgacca atgcccacag aatcagcatt cgatcctctc ccgtctctcc gtgtgtctcc
      181 acactcgctg ttactcaaac cggcaatcgc ctgcattcag gaatgcccgc atcgagaggg
      241 actccgcctc ctcggcaggc agagagcttt ccgccgcccg cggcatccgt ttacttcacc
      301 agaaaatcca ccaactgcac gtcccgcaac tgaaacgatt gagccgctgc gccggcgatc
      361 gctttccgga caatggcctc gatgtcggag tggagtgtcc gggtggtgaa agcctcttgt
      421 gctttctcca tcgtcatttt catcagaagt tgaacgacct gatcacgaat cgtctcggtc
      481 tgctgcgtga gaacggtctc atcccctttg taggaaatgg tcaggcccag ctttatctga
      541 tgcagttctt catctcgcgt cgtagaagtg atttcaccca gttcaaagat cttggcgtcc
      601 agttccgccg gcggagtgga caattcgtcc gagttttgtt ctaccggagc cacgatcgtt
      661 ggcccacgaa ccgatgacag tttccccgtc ttcttgtcga cgagaatgac ctcacccggt
      721 gttgctccac gtcccacaag gggtgtcgct tcccacgcgg ctcccttgtc cttgatctcc
      781 gcgatgccgc cgcagcccga cacgaagtgc agcaggtagt ctccggccag aacccgggct
      841 tcgtcctggc tcacgccgtc cttgagatcg accttcgcga acgtcgacag catctgggtg
      901 tcgacaattg tctgtcccgt ttcacgcgcc tcgatggccg aagtgagcat gaacaaaagt
      961 accagcacga cgcaaccgac tcctatccct ttcatgtctt ctctcctttt cgtgcacgtt
     1021 ctgaaactgg tgtcatcgcc agataccgcc cgcaatcagc gaccttcatc cttgcgcgtc
     1081 gagccgataa tcaccaactt cttgtccttt tccacagacg atttacctcg ggtctgcctc
     1141 ggactcttcg tcggggcagc agaagccaac tcgtagatgc ggacctgcgt cagacgcccg
     1201 tgaaaacgtc gccgaacctc ccggcaaaac tggaaccccg aattttcgag cgtacgaatg
     1261 gaagcgtgat tatcagccgg aaccaccccc agcaccagag gtaaccggta ctgttccatg
     1321 gcccacgcgg tcatggtatg aaccgcatcg gcggcataac catgtccctg ctgacactcc
     1381 tcgattgcgt agccgacttc aacatcagtc ttgagtgggc tcagaccgac atgtccgatg
     1441 agctcaccgg tacttttcag gcacacaccg aacaccagcg gtttatcggc cgggccgccg
     1501 ggtgactcgt actgttcgct gagaaacgtc aacacctcgc gggcatgggc gacactctca
     1561 tagacctgat ccggaatcca ttgtttcatc cccgactcga tgctcatgcg aaagactttt
     1621 tcggtgtctt cgagcgtgaa cggccgcaga tagacccgct tgccctcgac gcgttgcaac
     1681 acatcatgcc tcatcgtctt gccctcacga cttcgctcgt tgtctcactg aaatatcgcc
     1741 gtgcgaatac cagaccgcca gaaccgagat cgcttccata tcaggagcct cttccgcaca
     1801 tcggtcccaa aaccgccagg agaatcagga tggacaaaat gatcacgtat tccatgacgc
     1861 tccagaaact ccaaccggaa cggtctgtgt tctcggcctt cgtcccaggg gcgtgatgaa
     1921 cggcgaatcg gaaaccacat tccacacacg tttcagtctc ttgatccgga ttcattcggc
     1981 gtatcgatgt cccacaattc ggacaggaac tagctcctga cgactccagc ctctctgtca
     2041 tgtggtgccc ctctttctct gatcgtcgct caatcccccg gtcagcacag agtataacct
     2101 ttctcgtaac ttgtcacaca caatctgtga aaaggcaggg tcagtcaatt ctgtcctgtc
     2161 ctgccaaggg gaaaacccaa acccgagaat catatgaccc tggcgaaaag gttcaggaaa
     2221 gtggcggatc tctcttttgc cccctccgga aacgtcgatt tttgtttcta tatatttgga
     2281 aatagtttga catcgccgtc gaatcggctc atatttattt caacatctat tgaaatacag
     2341 cgaggtggtc atgaaagtcg acgaactggt tgccatcggg aaaggtctgg cagaccgcgc
     2401 ccgggtgcgc atgctccgct ctctgctgct caccccgcag tatctcgaag agctggctaa
     2461 gcgcctgaat ctcccgctct ccacgacctc gtttcatgtc aacaagctgg tcgaggccgg
     2521 gctggtctgc cgacgtcgcg agcagtacta tgccgtgttc gagatcccgc gtgacacgct
     2581 cccctcacca ctgatcgagc tgctcgcgaa cctcgatgat tcagagacgg gggaaagcac
     2641 gcgagtgcgg aaacttcgcg acgagactat caagcgctca ttccgcaacg gcgtgcttct
     2701 gaccattccg acccagaaga agaaacgcct gctggttctc gaagagatcg ccagacgttt
     2761 cgacgccgat cgagactatg ccgagaaaga tctgagccgc ctgctggaac gaatcaaccc
     2821 ggactattgt ctcctgcgac gcctgttggt cgatgagggg ttcatggtcc gctcgcacgg
     2881 ggtctatcgc cgggtccatc gcccgccggt cctgatcgaa aacgccgtcg gcccggcacc
     2941 gcgatctccg gcgcctccca ttcccccgtc acccgcgaac aaacgccttc gcaccccgcc
     3001 ttccacaaag gaccctcgta tgacgaccga tcgcaagcaa ctgaaacgcg aagccctcga
     3061 gcagaagccc gagatgggca tcttcaagat cgaaaaccgc acgaatggca agatctttct
     3121 cgtcgtcgcg cccaatattc cggcgtatat gacccggcac cgattcgacc tcgaactcgg
     3181 ctcacacccg aaccgggaac tgcaggccga ctggaagaag cacggaccgg acgctttcac
     3241 cttcgaggtg ctcgaaacca ttgatcagaa agatgaatcg agagaccaca acctggacga
     3301 actgaaaagc ctcggtgccc ggtatctgaa aaagctgcac ccctgggacg accgcggtta
     3361 ccacgaccgc ccgcgcgagc gccagatctc cgcccgcggc gaagactgat caagtcgcga
     3421 tcgagagcgg gaaccagcac gttctagacc tgttcacgat cgcgacttga tcagtcttcg
     3481 ccgcg
//
LOCUS       DAORHD010000418         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30143_length_3483_cov_3.392940, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000418 DAORHD010000000
VERSION     DAORHD010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30143_length_3483_cov_3.392940"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PKO06_09755"
     CDS             complement(<1..355)
                     /locus_tag="PKO06_09755"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV69972.1"
                     /translation="MKRTPRLEIYPKRIQKNTRSVAGLCHTHGVTLAAVTKVTCAYPY
                     VAHALVEGGADWLADSRPANLWSLREQGHRGPFLLLRLPYPSEAFDVVRAADYSLNTS
                     LVTMKALSDEALLLGR"
     gene            701..2317
                     /locus_tag="PKO06_09760"
     CDS             701..2317
                     /locus_tag="PKO06_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV69973.1"
                     /translation="MRRVSLALAAFLSAGTLIAGPYDDQAVSSAQKAKTGSTAGAAVS
                     VPSTGVLKQVATRYPIQRYPSLDAAFDALNSNERDVLAKVRYVWDNDDAWYTRWRMIS
                     DAKQTIDCTYYIVDKDIFGQSFLGLLCKKAREGVKIRLMVDGRIYRSPYMKGMADRFQ
                     ELARFSNVQIKLFNSVSQSLLHMFTDFKGLFASNHDKIIIADGKTSIIGGRNIGPDYY
                     GDKGEYPIIYRDSDILMEGNHITARLLKAFNTEWNGLRNSAVKPDRWNWNDQMDRLNI
                     ACMVMDRYMYGKGLIDPKKVTNWTKKQREILTELNEEISKFKKISGFADFELWNRATT
                     IAPVKVIDKESAIGKMNDITPCIVSFIDSANEEILIQNPYVVLTDQAYQALKRASARG
                     VKIIFHSNSGNSTDSLFPQAFLMNDWKQMLKDMPTCRILVAPSQNERLHSKVFVFDSK
                     ITIIGSYNMDPLSEDKNSEIVAVVNDAQFGAETMEQMHRDMAVVLEYTIKINPDGSVE
                     KVFGPEDHLNEKILKRMNLYRKLKWIRPVI"
     gene            2462..2986
                     /locus_tag="PKO06_09765"
     CDS             2462..2986
                     /locus_tag="PKO06_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69974.1"
                     /translation="MMVLPSLTLLRRSFSRRFGLALLVLFVCAAFVPAHAQSQYKNEK
                     FKFSIQVPVPEKTEIAAPYQLITMPLPSENGFSANVNVQIQPFTGTTEEYMKVSRDGL
                     QQMGVKITTEKSGDGWIMLEAEGEMQGFALHFYAKAWITKERTYLVTATCLQSEWAAK
                     KDELVTCVESFKTW"
     gene            complement(3180..>3483)
                     /locus_tag="PKO06_09770"
     CDS             complement(3180..>3483)
                     /locus_tag="PKO06_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69975.1"
                     /translation="LDAGVKLTGAAGKRIMRFGLQYPKWGARALKVAKKVGWDHADVT
                     LVALGELLVMIDTKLLMIAGLAVWLWCFWLDLLGQTWAFLCWLFRRSKQPEGVAAA"
BASE COUNT          776 a         1039 c          968 g          700 t
ORIGIN      
        1 gcctgcccag cagcagagct tcgtcagaga gggctttcat ggtgactaag ctcgtgttca
       61 ggctgtaatc ggcagcgcgc acgacgtcga acgcctcgga gggatacggc agccgcagca
      121 gcagaaacgg cccccgatgc ccctgttcgc gcaggctcca gaggttggcc gggcgtgagt
      181 cagccagcca gtccgctccg ccttcgacca gcgcgtgcgc cacatacggg taggcgcagg
      241 tcactttggt aacggctgcc agcgtgactc cgtgcgtgtg gcacaacccc gcaaccgagc
      301 gggtgttctt ctgaatgcgc tttggataga tttcgagacg tggtgtgcgc ttcatcgctt
      361 cctcctgcgt tttcaggcat tcgcgccccg cagggtagca caaaacgtcc caacatgcac
      421 cgtaccattg cggcgacagt atacatatta ttcgaaatac ggcacggcgg gcccggtcta
      481 ccactactct ccgttgaatt ggtctgcctc agatcacctg tctctcctga aatccccgcg
      541 cctctcatcc atctatcttt cgcacgcacg gatccaggaa aacaggagct cacgtgaagg
      601 aagcgcgttc cggtttgcct tggcgcagcg cgaagtttga cggttcgggg agaaatccgt
      661 acaattattg gcaactcatc tctgaatgcg ggggtacacc atgcgacgtg tctctttagc
      721 acttgcggcc tttctttccg ccggaaccct gatcgccggc ccttatgacg atcaagcggt
      781 atcgtctgcc cagaaagcaa aaaccggctc aacggccgga gcggcggttt ccgtgccatc
      841 gacaggagtg ctgaaacagg tggcaacgag atatccgatt cagcgctacc cctcactcga
      901 cgcagccttc gacgccctca acagcaatga acgtgatgtg ctggccaagg tgcgctacgt
      961 ctgggacaac gacgacgcct ggtatacccg ctggcgcatg atctccgatg ccaaacagac
     1021 catcgactgc acctactaca ttgtcgacaa ggacatcttc ggccagtcgt tccttggtct
     1081 gctgtgcaaa aaggcccgcg aaggggtcaa gatccggctc atggtcgacg gccgcatcta
     1141 tcgttccccg tatatgaaag gcatggccga ccgttttcag gaactggccc gcttttcgaa
     1201 cgtgcagatc aagctgttca actcggtttc gcagagcctg ctgcacatgt tcaccgattt
     1261 caagggcctc ttcgccagca atcacgacaa gatcatcatt gccgacggca aaacctcgat
     1321 catcggcggt cgcaacatcg ggccggacta ctacggcgac aagggcgagt atcccatcat
     1381 ctatcgcgat tccgatattc tgatggaagg gaaccacatc accgcgcggc tgctgaaggc
     1441 gttcaacacc gaatggaacg gcctgcgcaa ctcggcggtc aaacccgatc gctggaactg
     1501 gaacgatcag atggatcgcc tgaacatcgc ctgcatggtg atggaccggt acatgtacgg
     1561 caagggtctg atcgatccga agaaggtcac gaactggacg aagaaacagc gcgagattct
     1621 caccgagctc aacgaggaga tctcgaagtt caagaagatt tcggggtttg cggatttcga
     1681 gctctggaac cgcgcgacca ctattgcacc ggtcaaagtc atcgacaagg aatcggcgat
     1741 cggcaaaatg aacgacatca cgccgtgcat cgtgtcgttc atcgattccg ccaatgaaga
     1801 gattctgatt cagaacccgt acgtcgtgct gaccgaccag gcgtatcagg cactgaaacg
     1861 ggcctcggca cgcggcgtaa agatcatctt ccattcgaac agcggcaact cgaccgactc
     1921 gctcttcccg caggcgttcc tgatgaacga ctggaagcag atgctgaaag acatgcccac
     1981 ctgccggatt ctcgtcgccc ccagtcagaa cgagcgcctg cactcgaagg tcttcgtctt
     2041 cgactcgaag atcacgatca tcggcagcta caacatggat ccgctcagtg aggacaagaa
     2101 ctcggaaatc gtcgccgtcg tcaacgacgc gcagttcggc gctgaaacca tggaacagat
     2161 gcatcgcgac atggccgtgg tgctcgaata cacgatcaag atcaaccctg acggcagtgt
     2221 cgagaaagtc ttcggccccg aagatcatct caatgaaaag attctcaagc gcatgaactt
     2281 gtaccgcaag ctgaaatgga ttcgcccggt gatctgatca tcggcaccag tgagtcaagc
     2341 caagccccgg gagcacatcc cggggttttg tttttctcaa ccttgccagt tttttgtgtc
     2401 ggtttgcagt tcctgataca ctgtgactgt tcgtgcaagc tcaaattaca ggagaaaaca
     2461 catgatggtt cttccttctc tgaccctgct tcgacgatct ttcagccgac ggttcggtct
     2521 cgcattgctg gtcctcttcg tgtgcgcggc gttcgttcct gcccacgccc agagccagta
     2581 caagaatgaa aagttcaagt tctcgattca ggtgccggtg cccgagaaaa cggagatcgc
     2641 ggctccgtac cagttgatta ccatgccgtt gccctctgag aatggttttt cggccaacgt
     2701 caatgtacag attcagccgt ttaccggcac gaccgaagaa tacatgaagg tctctcgcga
     2761 cgggttgcag cagatgggcg tcaagatcac caccgaaaag agtggcgacg gctggatcat
     2821 gctggaagcc gaaggcgaga tgcaggggtt cgcgctgcac ttctacgcca aagcctggat
     2881 cacgaaagag cgcacctatc tggtcaccgc aacctgtctg cagtccgagt gggcggccaa
     2941 gaaagacgaa ctcgtcacct gcgtcgagag tttcaagacc tggtaaattc gaatccggtc
     3001 ctgaccgact gaccgggcga agacaccggc aggtctgctt cagaagcagg cctgccggtg
     3061 tcgctttttc gttggcagtg ggtccagggg ccacgggctc ctggcggggt ggcgcggagg
     3121 ggcggagccc cttcattcgt tgccgccggc gcgcgctctc cgaggctcag gcttctgcct
     3181 caggcagccg caaccccctc gggttgtttc gagcggcgaa acagccagca cagaaaggcc
     3241 cacgtctgtc cgagcaggtc gagccagaaa caccagagcc agaccgccag accggcgatc
     3301 atcaacagct tggtatcgat catgacgagc agttcgccaa gcgcaaccag agtcacgtca
     3361 gcgtggtccc agccgacttt cttcgcgact ttcaaggcac gcgcgcccca cttcgggtat
     3421 tgcaggccga agcgcatgat gcgcttaccg gcggcaccgg tgagtttcac cccggcatcg
     3481 agc
//
LOCUS       DAORHD010000419         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30248_length_3475_cov_3.826901, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000419 DAORHD010000000
VERSION     DAORHD010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30248_length_3475_cov_3.826901"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /gene="asnB"
                     /locus_tag="PKO06_09775"
     CDS             complement(<1..1525)
                     /gene="asnB"
                     /locus_tag="PKO06_09775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV69976.1"
                     /translation="MCGICGRAHLLTGRAVSPESLERMLLHLHARGPDGEGTWISGSV
                     GLGHRRLAVVELSERAAQPVGILDEQIVGVFNGELYNALALRERLRQQGVTLNHRGEM
                     ELILWAYVIYDEAFFEHLDGMFALAIWDNRHRRLLLARDRFGKKPLFYRLDHDGIAFG
                     SLLASLNDVAVSPLMFDPIAIDQFLSLNYIPSPATGFLETRKLAAGECLTFDAAGVKV
                     RPWVAPSAHSGGAWRLADAERVLLDRLDRAVEKRLRSDVPVGLFLSSGLDSAAVLASL
                     HQLGASDIPAFTAAFKESGYDESEVASRLATHFRFPHVPIVVSPPPLETILQQLVHSC
                     GEPVGDSSLLPTWLLAQSASERVTVVLNGDGGDECFGGYHRHLASHWVERSGVWGLPL
                     AGLFGWAAHLVGQTGMDSGSTCGRWLSRLERLLLNLEMPLDDQYIRWMLLSNAKDSLY
                     LRGERLRGQGSSDGGREFLKECLRQCCSRGWLERLLEVEWKTYLGEDLLVKMDRACMA
                     "
     gene            complement(1690..2832)
                     /locus_tag="PKO06_09780"
     CDS             complement(1690..2832)
                     /locus_tag="PKO06_09780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV69977.1"
                     /translation="MESRDRLRILLIIGTLDIGGTERQLVELACGLNPLRFAPVVVCL
                     SAGGPFHERLLRHGVPVHILGFRGIQWDPIGGVRELYRLVRLIQSEKPHVIQVFLYWA
                     CVVGGLAARLAGHRHVIAARRSLGTFKSGKPLLLLAERLVNRWVRLFVANSEAVKEDV
                     CRRERVDRQQVTVIYNGYCQATQESELVTAESGIQKHCTGSAPVVAVLANFIHYKGHT
                     WFLDAWRQVVARLPSAKAVLIGDGPMRPAIEALLAQYGLNNSVILTGRCINPGVWLKR
                     ADLLVHTSSEEGCCNAILEGMALGLPIVAADVGGNTELVQDGKQGRVISYGDTTALVQ
                     AICHLASEPVERSRMGAAGVETVSRMFTMARMCAEYASLYERISRI"
     gene            complement(2819..>3475)
                     /locus_tag="PKO06_09785"
     CDS             complement(2819..>3475)
                     /locus_tag="PKO06_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HNV69978.1"
                     /translation="GRLPVRTARALLVSLLVVSLGVSIFFPPAFMAATAGYFLLNLAY
                     SFWLKNQVILDVLTIAIGFVLRALAGVGAMQALDPLVKMSHWLVLCTLMLALFLALAK
                     RRGEIWRMRSEAASHRRILEEYSLHFLDEMTAVAGATTIIGYALYTVSAETIARIGSD
                     RLIFTVPFVIYGVFRYLYLIHIRNQGENPSSVVLHDRPMQINLALWIVAVILLIHGIS
                     "
BASE COUNT          850 a         1088 c          913 g          624 t
ORIGIN      
        1 gtgccatgca tgctcggtcc attttcacca gcaggtcttc gccaagatag gttttccact
       61 ccacctccag caggcgttca agccacccac ggctgcaaca ttgcctcagg cactctttca
      121 ggaactctcg cccaccgtcc gacgagccct gtcctcgtaa acgctcgccg cgcaggtaaa
      181 ggctgtcttt agcgttcgac agaagcatcc agcgaatgta ctggtcatca agtggcattt
      241 ccaggttcag aagcaatctt tcgaggcgtg atagccatcg accacatgtc gatccactgt
      301 ccattccggt ctgaccgacc aaatgagccg cccaaccgaa caggcctgcc aagggaagtc
      361 cccagacccc cgatcgttcc acccaatgcg aagccagatg ccggtgatag ccaccgaagc
      421 attcatcccc cccgtcacca ttcaagacga ccgtcacccg ttcggaggca gactgagcca
      481 gcaaccaggt gggcaacaac gacgagtccc ccaccggctc accacatgaa tgtacgagtt
      541 gctgaagaat cgtttccagt ggcggcgggg aaacaacgat cggcacatgc ggaaagcgaa
      601 agtgcgttgc cagccgactc gccacctcag actcgtcgta cccagactct ttgaatgccg
      661 cagtaaacgc cggaatgtct gatgctccga gttgatgaag agaagcgagc accgcagctg
      721 aatccaaccc ggaactgaga aacaacccga cgggaacatc actgcgcaga cgtttttcca
      781 ccgcccggtc gagtcgatca agcaacaccc tctctgcgtc agccagtctc cacgccccgc
      841 cggaatgagc cgaaggggca acccacggtc gcactttcac gcccgccgcg tcgaacgtca
      901 ggcattcacc tgccgccagc ttccgagtct caagaaaccc cgtcgccggt gacgggatgt
      961 aattcagtga caggaactga tcaatcgcta tcgggtcgaa catcagcggg gacacggcta
     1021 catcgttcag gctggccaac agagagccga aagcgatgcc atcgtgatca agccggtaga
     1081 acagcggctt tttgccgaaa cgatcacgcg ccagcaacag ccgccggtgt cgattgtccc
     1141 agatggccag ggcaaacatg ccgtcgaggt gctcaaagaa ggcctcgtca tatatcacat
     1201 atgcccagag aataagttcc atctccccgc ggtggttcag tgtcactccc tgttgccgca
     1261 ggcgttcgcg cagtgccaga gcgttataca gttcgccgtt gaagacgccg acaatctgtt
     1321 cgtcaagaat gccgaccggc tgtgccgctc gttctgaaag ctcgacaacc gccagtcgcc
     1381 gatgacccag gccaacagag ccagagatcc aggttccctc accatcgggg ccgcgcgcat
     1441 gcagatgcag cagcattctt tcaagagact cgggggatac agcccgtcca gtcagcagat
     1501 gtgcccggcc acagattcca cacatggatc aaatcaccac ttcagaaccg gtgagggaag
     1561 gactccatcc gaagctcttc gtacgcgtcc cagtcgcttc ccgatcatac atgcgaccag
     1621 aagagaaagc agggcattcc gcgccctcgg ggtcgcgagc catcttcatc tggcgccacg
     1681 gtcgcaggtt catatgcggc tgattctctc atacagcgat gcgtattcag cacacatgcg
     1741 cgccatcgtg aacatgcggg aaacggtttc cactcccgcg gctcccatgc gagatcgttc
     1801 gacaggttca gaagccagat gacagatggc ttgcacgaga gctgtcgtgt ctccatagga
     1861 gatcacacgt ccctgtttcc catcttgtac caattcggtg tttccgccga catctgccgc
     1921 cacaatggga agtccgagcg ccattccttc aagaatggca ttgcaacagc cctcttctga
     1981 tgaagtatgg acaagaaggt cggcacgttt cagccacact cccgggttga tgcaacgccc
     2041 cgtcagaatt accgagttgt tcaagccata ctgagcaagc aaagcttcga tggcagggcg
     2101 catgggacca tcaccaatca gcacggcttt cgccgaaggc aggcgggcga cgacctgacg
     2161 ccaggcatcg agaaaccagg tatgtccctt atagtggatg aaattggcca gcaccgctac
     2221 gaccggcgcg gagcctgtgc aatgtttttg aataccactt tcggcagtca ccaactcgga
     2281 ctcttgtgtc gcttgacaat acccgttgta gatcacggtc acctgctgac ggtccacccg
     2341 ctcacgacgg cagacatctt ctttcaccgc ctcggaattc gcgacgaaca ggcgaaccca
     2401 tcggttcacg agacgttcag ccagcagcag gagaggcttt cctgacttga aggtgcctag
     2461 actgcgacga gccgcgatca cgtgacgatg tccggccaga cgcgccgcaa gcccgcccac
     2521 gacacaggcc caatacaaaa acacctgtat gacatgaggc ttctcgcttt gaatgagtct
     2581 gacgagacgg tacagttcgc gaacacctcc gatcggatcc cactgaatac cacgaaaacc
     2641 cagaatgtga actggaacgc cgtgtcggag cagtctctca tggaacggtc ctcccgcaga
     2701 caggcagacc acgacaggag caaaacggag agggttcaga ccacacgcca actcgaccag
     2761 ttgtctctcg gtgccgccaa tatccagggt tccgataatc agcagaatcc gaagccggtc
     2821 acgagattcc atgaatcaac aggatcacgg caacaatcca gagagcgaga ttgatctgca
     2881 tgggacggtc gtgcaacacc accgacgatg gattttcccc ctggttccga atgtggatca
     2941 ggtagaggta acgaaaaacg ccgtagatca caaacggcac cgtgaaaatc agacggtcag
     3001 agccgatacg ggcaatcgtt tcggcgctca ccgtatacag tgcatacccg ataatcgtgg
     3061 tcgcgcctgc caccgctgtc atttcgtcca gaaagtgcag tgagtactct tcgagaatcc
     3121 gccgatggga agccgcttcg ctacgcatgc gccagatctc gccgcggcgc ttcgccagag
     3181 ccagaaacag cgccagcatc agggtgcaca acaccagcca gtgcgacatc ttgaccagcg
     3241 gatcgagggc ctgcatggca ccgacaccgg caagcgctcg caggacgaag ccgatggcga
     3301 tagtcaggac atccagaatg acctggtttt tcaaccagaa ggagtatgcc agattgagca
     3361 gaaagtatcc ggccgtcgcc gccatgaacg cgggcggaaa gaaaatggaa acccccaggc
     3421 tcacgaccag caacgacacg agaagcgctc gtgccgtccg caccggcaat cttcc
//
LOCUS       DAORHD010000420         3470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30319_length_3470_cov_3.410835, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000420 DAORHD010000000
VERSION     DAORHD010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30319_length_3470_cov_3.410835"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2105
                     /gene="pnp"
                     /locus_tag="PKO06_09790"
     CDS             <1..2105
                     /gene="pnp"
                     /locus_tag="PKO06_09790"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNV69979.1"
                     /translation="FKREGRPTAKEILTSRLIDRPLRPMFPEGYYNEVQICAMVLSYD
                     LIHPVDILAMNAASCALTISEIPFYNPVAAVRVGKIDGQLVVNPSPAQLDKSTIDMTV
                     AGSEKAITMVESGSSQVSETELLEALAFAHENIKKLVAAQKELQSKLGKTKKTVEPPA
                     RDEELYGMLKEAVFPIIKDALQTAEKTERKTKFSAAKEKMQTLVKGKYTDDKECLLKL
                     KAAGRIFEDMIYVEMRRMILEDSRRIDGRSLTDIRQITPAVGFLPRTHGSALFTRGQT
                     QSLGIATLGAKSESQIIDGIDEEYKKPFILHYNFPPFSTGETKPMRGPGRREIGHGAL
                     AERALQAVVPPPEEFPYAIRVVSEILSSNGSSSMASVCSGSLALMDAGVPLKGAVAGI
                     AMGLIKEGENYKVLSDILGDEDHLGDMDFKVAGTTKGVTALQMDIKIDGVTLDIMRTA
                     LEQARHGRLHILDKMNAVISTHRTELSPYAPRIVTIQIDPDKIRNVIGPGGKMIRQIQ
                     DETKTVIEIEGDGRVHVVSPDVISNNRAVEMIKRLTETAEVGKTYTGKVKRIMKFGAF
                     VEILPGVEGMVHISQLAHERVNTVEDIVNIGDETTVKVIEIDSMGRINLSRKVLLPEP
                     AGYVRPAEGEDQPRDDRPPRGDRPRFDRPRRDDRPRGDRHHGGGNRDRNFDRGPRPDR
                     PHNPDREGGEGGNQGGQD"
     gene            complement(2232..2744)
                     /locus_tag="PKO06_09795"
     CDS             complement(2232..2744)
                     /locus_tag="PKO06_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69980.1"
                     /translation="MNAYASYMQKALEKRPDFGGAPVPVNAQYDEVMRLARQTPEGMQ
                     QARLFFARGNYSAAVQEFQTVLGTLQENDLQNRISIHQALAECFFQQNIRIAYVEHKA
                     KALELTQRLRDMLRGGAQQSPLWMSVDEATQVLLKVRVHALQNLKGREKEDAIRRAEF
                     DLEVARVLHR"
     gene            complement(2741..2995)
                     /locus_tag="PKO06_09800"
     CDS             complement(2741..2995)
                     /locus_tag="PKO06_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69981.1"
                     /translation="MDTSRWPFVLAVVVLLALIYNFLNTIFRSDVKRFSHDRLYIDYA
                     SYKKKETKKPSGSSSSPSPFVAPQLRTSFARFSTVAWKIR"
     gene            complement(2999..>3470)
                     /locus_tag="PKO06_09805"
     CDS             complement(2999..>3470)
                     /locus_tag="PKO06_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69982.1"
                     /translation="RDRFIKVMKIDYTWNPADGTVTREQKPLYLYFLRNPMETRDQPQ
                     TEWEGDPSATPGKKVMLHNIADFIFEGYEQKFDYAKPKTENPIAIRKIDGAIANDARR
                     TTFVVVRVHTKIDEKAGDRKDEELDLVAKFYSRVRLADAAYPGYFSQTDEIGAF"
BASE COUNT          729 a         1069 c         1024 g          648 t
ORIGIN      
        1 tcttcaaacg cgaagggcgc cccaccgcga aagagattct caccagccgt ctgatcgatc
       61 gccccctgcg gccgatgttc cccgagggct attacaacga agtgcagatc tgcgccatgg
      121 ttctgtccta cgatctgatc caccccgttg atatcctcgc catgaacgcc gcctcctgcg
      181 cgctgacgat ctccgagatt ccgttctaca atccggtggc cgccgtccgc gtcggcaaga
      241 tcgatggtca gctcgtggtc aatccttcgc cggctcagct cgacaagagc acgatcgaca
      301 tgaccgtcgc cgggtccgag aaggcgatca ccatggtcga atccgggtct tcgcaggtct
      361 ccgaaaccga actgctcgaa gcactggcgt ttgcgcacga gaacatcaag aagctcgtgg
      421 ccgctcagaa agagctgcag tccaagctcg gcaagaccaa gaagacggtt gaacccccgg
      481 ctcgcgatga agaactctac ggcatgctca aagaggccgt cttccccatc atcaaagacg
      541 ctctgcagac ggccgagaag accgaacgca agaccaagtt cagcgccgcc aaagaaaaga
      601 tgcagactct ggtcaaaggc aaatacaccg atgacaagga atgcctgctc aagctgaaag
      661 ccgccggtcg catcttcgaa gacatgatct atgtcgaaat gcgccgcatg atcctcgaag
      721 acagccgccg catcgacggg cgcagcctca ccgacatccg gcagatcacg ccggccgtcg
      781 gctttctgcc ccgcacgcac gggtcagcgc tcttcacccg cggtcagacg cagtctctcg
      841 ggatcgcgac cctgggcgcc aagtccgagt cgcagatcat cgacggcatc gacgaagagt
      901 acaagaagcc gttcattctg cactacaact tccccccgtt ctcgaccggt gaaaccaagc
      961 ccatgcgcgg gccgggtcgc cgcgagatcg gtcacggcgc gctggccgaa cgcgcgttgc
     1021 aggcggttgt tcctccgccc gaggaattcc cgtacgcgat tcgcgtggtc agcgagattc
     1081 tgtcgagcaa tggcagttcg tcgatggctt ctgtctgctc gggttctttg gccctcatgg
     1141 atgccggcgt tcccctcaag ggagcggtgg ccggaattgc catgggcctg atcaaggaag
     1201 gcgagaatta caaggttctc tctgacatcc ttggcgacga agatcacctc ggcgacatgg
     1261 atttcaaggt ggccggcacg acgaagggcg tcaccgctct gcagatggat atcaagatcg
     1321 acggtgtcac cctcgacatc atgcgcaccg ctctcgaaca ggcgcgccat ggtcgtctgc
     1381 acattctcga caagatgaac gcggtcatca gcacgcatcg caccgagctc tcgccctatg
     1441 ctccgcgcat cgtcaccatc cagatcgacc cggacaagat ccgcaacgtc atcggacccg
     1501 gtggcaagat gatccgccag atccaggatg agacgaagac cgtcatcgag atcgagggag
     1561 atggccgcgt gcacgtggtc agccccgacg tgatctcgaa caatcgggcc gtcgaaatga
     1621 tcaagcgcct gaccgaaacc gcggaagtcg gcaagaccta caccggcaag gtgaagcgca
     1681 tcatgaagtt cggcgcgttc gtcgagattc tgcccggcgt cgaaggtatg gtgcacatca
     1741 gccagctcgc gcacgagcgt gtcaacaccg tcgaagacat tgtcaacatc ggtgacgaga
     1801 cgaccgtgaa ggtcatcgag atcgacagca tgggccgtat caacctgtcc cgcaaagtgc
     1861 tgttgcccga gcccgccggc tacgtgcgcc ccgcagaagg cgaagaccag ccccgcgacg
     1921 atcgaccgcc ccgtggcgat cgacctcgct tcgaccgccc gcgccgcgat gatcgccccc
     1981 gtggcgatcg gcatcacggc ggcggcaatc gcgatcgcaa cttcgatcgc ggtccgcgtc
     2041 ccgaccgccc gcacaacccc gaccgcgaag gcggcgaggg tggtaaccag ggcggccagg
     2101 attaaaagtc cgagtcgttg aagccgaaat acggcttcac aagctgaaaa gccggggaga
     2161 tccagattgt tgggtctccc cggttccctt ttccggcacc cccgcaggcg ccggacaggc
     2221 aggaggggag actagcggtg caggacgcgg gcaacctcga ggtcgaactc ggcgcgcctg
     2281 atggcgtctt ccttctcgcg acccttgaga ttctgcaggg cgtgaacccg cactttgagc
     2341 agcacctgcg tagcttcgtc gacgctcatc cagagcggag actgttgcgc acccccgcgc
     2401 agcatgtcgc gcagccgctg cgtgagttca agcgctttgg ccttgtgttc gacataggcg
     2461 atgcgaatgt tctgctgaaa gaaacattcc gccagggcct gatgaatgct gatgcggttc
     2521 tgcagatcat tttcctgcaa cgtgcccaga acggtttgaa actcctgtac cgcggcactg
     2581 tagttgccac gggcaaagaa caggcgagcc tgctgcattc cttccggggt ttgtcgggcc
     2641 agtcgcatga cttcgtcata ctgggcgttc acggggaccg gagcaccccc gaagtcgggg
     2701 cgcttttcca gcgctttctg catataagat gcgtaagcgt tcaccgaatc ttccatgcga
     2761 ccgtcgagaa cctggcgaaa ctcgtacgca actgcggggc gacgaaaggt gatggcgatg
     2821 acgagcttcc agagggcttc ttagtctctt tcttcttgta cgaagcgtag tcgatataca
     2881 gccgatcgtg agagaaccgt ttcacatcgg agcggaagat cgtattgaga aaattgtaga
     2941 tcagcgccag cagcacgaca accgcgagaa caaagggcca gcgggaggta tccacgggtc
     3001 agaaggctcc gatctcgtca gtctgtgaga agtagccggg gtaggcggca tcggccagcc
     3061 gaacgcgact gtagaacttc gccaccagat cgagttcctc gtccttgcgg tcgccggctt
     3121 tctcgtcgat cttggtgtgt acgcgcacca cgacgaaggt tgtgcggcgg gcatcattgg
     3181 cgatagcgcc atcgatcttg cgaatcgcga tggggttctc ggtcttgggc ttggcgtagt
     3241 cgaacttctg ttcgtagcct tcaaagatga aatcggcgat gttatgcagc atcaccttct
     3301 tgcccggagt ggctgacgga tcgccctccc actcagtctg cggctggtcg cgggtttcca
     3361 tcgggttgcg cagaaagtag agatacagcg gcttctgctc gcgggtcacg gtgccgtccg
     3421 ccgggttcca ggtgtagtca atcttcatca ccttgatgaa acggtcgcgg
//
LOCUS       DAORHD010000421         3467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30371_length_3467_cov_3.062427, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000421 DAORHD010000000
VERSION     DAORHD010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30371_length_3467_cov_3.062427"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKO06_09810"
     CDS             complement(<1..315)
                     /locus_tag="PKO06_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69983.1"
                     /translation="MKSNLCRLVLLFIAVLSLLAVPAEAAVVADPDAVEVLGLICRDD
                     DGAAWVKAGGQEILVTPGFMLARDVRVTAIQPDGIVMYRPKARTYHVAKLKLPERTRK
                     TRT"
     gene            418..1050
                     /locus_tag="PKO06_09815"
     CDS             418..1050
                     /locus_tag="PKO06_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69984.1"
                     /translation="MRQLSLRVLVLGVVLLGLVALTPGYAQTPAPTTMEVPVKYDLGI
                     ASAPPIDGFITGGAICEPEGLSLIPADATEPVLFQYQDIDEAKDVALKGTDEVTLKLK
                     MRDGKEVTASAKVTDAEPILIVRDEVAAGADPATAKVRTFQTTNFRGIPLIEFEAIPE
                     VEVDVEAIKKMNEEFLKALETGDFDKAVDQYNAMGDALDKAQGDGEPAAP"
     gene            1175..2500
                     /locus_tag="PKO06_09820"
     CDS             1175..2500
                     /locus_tag="PKO06_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69985.1"
                     /translation="MSRCIARILVLSVLLLGATGVWAFDPASVYPENGFLFVRVFVKD
                     LLPTGDNWSKDMPEFGRVFDKTTKLLTDKVGFHPMRDIHEVGLFITSEIDFQKRKPNQ
                     IAVFVRGAFQPDRLLALLPDLLSGMGPKAQLISIKDVQGRKVVSGPEFQAFFFDNETL
                     LAGTPDVIEAVMAGKLALGKAPDALKNTLTSSRFFLSADAKTLKEKSPKVKRDLERVP
                     PPMRVHLENLLQLVLAYADGRVAVKADFSQAESAAFIASSLDAIKNQGLLECESRLKT
                     LEANLEQMSATELLGEKRSQMLALAAGKDVIANLTSKASGNTFEASFLIPEPVRSLGA
                     NPAMVPLIGILSAVAIPNFKRAREKALTTACFANQRILLGALEMYNMDHQDMKTSITD
                     ADGAEGSELIKGKYLPSPIKKSDPECEYSSEGDLSAGGKIRCRKHGSLF"
     gene            complement(2587..3228)
                     /locus_tag="PKO06_09825"
     CDS             complement(2587..3228)
                     /locus_tag="PKO06_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69986.1"
                     /translation="MRFDLTPFEQELKELLARFEESRFISTLPVKKRDQTVREMAESG
                     DGDAQAYLAWLQMGGAYGSYARDVSTTPDLLSAATWARRSAKQNNLSGHAVLFLLLFR
                     GGPGLEPVREEAVKSLRVLAEREWAEAQALLGECCYMGKGMPENNAEALKWFRLAIQN
                     GCWRAGNNLADMYEYGHGVPENREEAVRLRMQAAKSSPAHPYASRHKIGDDGL"
     gene            complement(3257..>3467)
                     /locus_tag="PKO06_09830"
     CDS             complement(3257..>3467)
                     /locus_tag="PKO06_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69987.1"
                     /translation="NTQGQRLNTWGQDRLNQGGVGNTIVGGLAVAGGHVTQGVAQAGK
                     FVSDAWSAGTNIVGNGIKRLGSLFD"
BASE COUNT          747 a         1062 c          965 g          693 t
ORIGIN      
        1 ggtgcgggtc ttccgcgtgc gctcgggcag cttgagtttc gcgacgtgat acgtgcgcgc
       61 cttggggcga tacatcacga taccgtcagg ctgaatcgcc gtcacgcgca cgtcacgggc
      121 gagcatgaat ccgggggtga ccaggatctc ctgaccgccg gccttcaccc aggcggcgcc
      181 gtcatcgtcg cggcagatga gccccagcac ttcgacggca tcgggatcag caacgacggc
      241 agcctctgcc ggcacggcca gaaggctcag cacagcgatg aacagcagaa caagacgaca
      301 cagattcgat ttcatggatc caccccgatc aaaacttgct cgaacgagcc cggccagttt
      361 accatatctc cgccgggaaa cacagtcaga cacatccggc tacaggagaa acagaacatg
      421 cgtcagttgt cccttcgggt cctcgttctt ggagtcgtac tgctggggtt ggtcgccctg
      481 acccctggtt atgcccagac accagctccc acaaccatgg aagtgccggt gaagtatgat
      541 cttggtatcg cgagcgcacc cccgatcgat gggttcatca ccggcggcgc catctgtgaa
      601 cccgagggtc tcagcctgat ccccgctgat gccaccgaac ccgtgttgtt tcagtatcag
      661 gacatcgacg aagccaaaga cgtggccctc aaaggcaccg acgaagtcac gctcaagctc
      721 aagatgcgcg acggcaagga agtcaccgcc tcggcgaaag tgaccgatgc tgaaccgatt
      781 ctgattgtcc gagatgaagt cgccgccggt gctgatccag ccaccgccaa agtgcgtacg
      841 tttcagacca ccaacttccg cggcattccg ttgatcgagt tcgaagcgat cccggaagtg
      901 gaagtcgacg tcgaggcgat caagaagatg aacgaagagt ttctaaaggc ccttgagacc
      961 ggcgacttcg ataaagccgt cgaccagtac aatgccatgg gcgacgctct cgataaggct
     1021 cagggagacg gagaacccgc agccccctga aacagttcac gatgaccaat caagaggttg
     1081 gtgtcagttg atctcgtaac cgatgctgcc tcgacacgtc tcgttcacga ccgcaggctc
     1141 gagactgaaa tgtgattccc aggaggaacc attcatgagt cgctgtattg cccgtattct
     1201 tgttctgagc gttctgctgc tcggcgccac gggggtctgg gcgtttgatc ccgcatccgt
     1261 gtatcccgaa aacggttttc tgttcgtgcg tgtcttcgtg aaagacctgc tgccaaccgg
     1321 tgacaactgg agcaaggaca tgcccgagtt cggaagagtt tttgacaaaa cgaccaaact
     1381 gcttaccgac aaagtcggat ttcatccgat gcgcgacatt cacgaggtag gactctttat
     1441 cacctcggag atcgactttc agaaacgcaa gccgaaccag atagccgtgt ttgtgcgcgg
     1501 cgctttccaa cccgatcgtc tgctggcctt gctgcccgac cttctgtcgg gcatggggcc
     1561 caaagctcag ttgatcagca tcaaggacgt gcagggacgc aaagtggtga gtgggcccga
     1621 gtttcaggcg ttcttctttg acaacgagac cctgctcgcc ggcacccccg acgtcatcga
     1681 agcggtcatg gccggcaaac tcgctctggg aaaagccccg gatgcgctca agaacacgct
     1741 gacctcttcg cggtttttcc tcagcgctga tgccaagacg ctcaaggaaa aaagcccgaa
     1801 ggtcaagcgc gacctcgaga gggttccgcc tcccatgcgc gtgcatctcg aaaacctgct
     1861 gcagctcgtg ctcgcgtatg ccgatggtcg agtcgcggtc aaggcagatt tcagtcaggc
     1921 ggaatcggcc gccttcatcg cttcaagtct cgatgccatc aagaaccagg gcctgctcga
     1981 atgcgagtct cgtttgaaga cactggaagc gaatcttgaa cagatgtcgg ccacggaact
     2041 gctgggggaa aagcggagtc agatgctggc gctggccgcc ggcaaggacg tcatcgcgaa
     2101 tctcacttca aaggcttccg gaaacacttt tgaagcatct ttcctcattc ccgaacccgt
     2161 acgcagcctt ggcgccaatc ctgccatggt gccgctgatc ggcattttga gcgctgtcgc
     2221 gattcccaac ttcaagagag cccgcgagaa ggctctgaca accgcctgct ttgccaatca
     2281 gcgcattctg ctgggggcgc tcgaaatgta caacatggac catcaagaca tgaagacctc
     2341 gatcacagac gctgacggcg cggagggaag cgaactgatc aaagggaagt atctcccatc
     2401 accaatcaag aagtctgatc cggaatgcga gtattcatcc gagggcgact tgtctgccgg
     2461 cggtaaaatt cgctgtcgca agcacgggag cctgttctaa gagcagtttg ctccagatac
     2521 aactcggaca caacgggcgc gggagtcatc ccgcgcccgt tgtcggtcaa gtcagattcg
     2581 cttcacttac aggccgtcgt caccaatctt gtgacggctc gcatacggat gcgccggtga
     2641 agactttgct gcctgcatgc gcagtcttac agcctcttcg cggttttccg ggaccccatg
     2701 accgtattcg tacatgtccg ccaggttgtt ccctgcccgc cagcagccat tttgaatggc
     2761 cagacgaaac catttcaggg cctcggcatt gttttcgggc attcccttac ccatgtagca
     2821 gcactcacca agtaacgcct gggcctcggc ccactctcgc tccgccagca cccgcaggct
     2881 cttcacggct tcttcccgga ccggctcaag accgggccca cctcgaaaca gcagcaggaa
     2941 caataccgcg tggcccgaaa ggttgttctg cttggcagat cgtcgcgccc acgtcgcggc
     3001 cgacaggaga tcgggagtgg tcgacacatc gcgggcatag gatccgtagg cgccgcccat
     3061 ctggagccaa gccagatagg cttgcgcgtc gccatcgccc gactcggcca tctcacgcac
     3121 ggtctgatca cgttttttga ccggtagcgt gctgatgaac cggctttctt caaagcgcgc
     3181 cagcagctct ttcagttcct gttcaaatgg agtcagatca aaacgcatga gactctctga
     3241 tgttgccggg gtgtaatcaa tcgaacagcg agccgagacg cttgatgccg ttgccaacga
     3301 tgttcgtgcc cgccgaccag gcatcagata cgaacttccc agcctgagcc acgccctgcg
     3361 tcacatgccc gcccgccacg gccagaccgc cgacgatggt gttgcccact ccaccctggt
     3421 tcaggcggtc ttgtccccag gtgttgagcc gctgtccctg cgtgttg
//
LOCUS       DAORHD010000422         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30383_length_3466_cov_3.493990, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000422 DAORHD010000000
VERSION     DAORHD010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30383_length_3466_cov_3.493990"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKO06_09835"
     CDS             <1..1359
                     /locus_tag="PKO06_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV69988.1"
                     /translation="DIADKARYSLAWCHIKLGTFDSAVTLLEQLSDNTAAPNRREAVK
                     NLVDLLMNLRKFPEAISWMRKAAIILGGDEAIEMDYMRGLALSRMGEFPEALEAFRDF
                     AKRHPKQVRSQEARYQASLMYITMGKFREALKELEPLLRREVLPEVREKAMYRTGECY
                     LNLGNLKNARDSFDRVLREYPQGKARLDTLYQLGEVEYQSGNYAGALEAFRTLGAAGG
                     EVSAQAQFRAGEVLMKAGRYTDAIKDFETYLANFGSAPLVDDARFKIGLCYLELKDQG
                     KALAAFSQLRESKGYFRQEARFQIGEIARGLGNYPLAIQQYQAILAEEPKHPLASRTR
                     RAIGICLHLAKDYPAAATMFLDILREYPATDVAVPESRFWLGKNLIAQGRVEDGILEL
                     LKVPVLYPKSPLVTEAYAEAARAYQSQGLTQRAEKMWRELLKVNNKGPLAEEARGHLK
                     KP"
     gene            1528..3153
                     /locus_tag="PKO06_09840"
     CDS             1528..3153
                     /locus_tag="PKO06_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV69989.1"
                     /translation="MRAIRKTALWAPLWAAFLLVPLVEVTAKDTYDLGKVQVVGQDAQ
                     STSMTPDSDTISQEMGERSELTPEIPLTEEKEVEKPVQKPTAPETVKTRTGGFTMGMA
                     KGTRNSFEVYGKGHGYQNGYIGSLSVGRSSRDGFRSTRSEDRTWADLSLHGNEGSYDL
                     DVAGTLATDKFAQRGWRTNPSPDAAIEDAPRRLIVKGSSTLPDGAYFQAEAAVDSITR
                     TTSNRPFGVNEEATLSSGRVGAEYASKLSEAVLGKVGFDIRRDSFGLQNGPEQDLTKR
                     NLRLSAQHRFERGSSIELGLKSLGLMNRERTAPMFRLDYRMKEPWQLILSYDEDLGND
                     SLEKVFLPRQYVGFATLRASHQKRTSAKVNYRSPEQFHLGLELFQESEDQGVEYVDAH
                     DVARNLLTSQIRFADDAKRRGMKVSGSVKLDEHFVLKAGATFQNPEDTATGRQLSYEA
                     KRLFDVELAYEHRSLKFNINRKSESDRVAYTPVTKVGLEDYSRTDLECKYQFDKHFSA
                     FVRVNDLYDEAQEKRYNVPEEGRVSLLGVEAQF"
     gene            3268..>3466
                     /locus_tag="PKO06_09845"
     CDS             3268..>3466
                     /locus_tag="PKO06_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69990.1"
                     /translation="MGSGWFFVDVFVKGGPVMIPILLCSIVALAIVIQRYRFFQSITL
                     SGEGFMRRVKSALSGRDYVEAM"
BASE COUNT          744 a         1004 c         1006 g          712 t
ORIGIN      
        1 gatattgccg ataaagcccg ctactcgctg gcctggtgcc acatcaagct gggtactttc
       61 gattcggccg tcacgctgct cgaacagctc tccgacaata cggccgcacc taatcggcgc
      121 gaggcggtga agaacctcgt cgatctgctg atgaatctgc gcaagtttcc cgaagccatc
      181 agctggatgc ggaaagccgc aatcattctc ggcggcgatg aagccatcga gatggactac
      241 atgcgcggcc tggccctgtc acgcatgggc gagttccccg aggcgctcga ggcatttcgt
      301 gatttcgcca agcgtcaccc caaacaggta cggtcgcagg aagcgcgcta tcaggcgtcg
      361 ctgatgtata tcaccatggg gaagttccgc gaagcgctca aggaactcga acccctgctg
      421 cgccgcgaag tgctgcccga ggtgcgcgaa aaggccatgt atcgtaccgg cgaatgctac
      481 ctcaatctcg gcaatctcaa gaatgcccgt gattcgttcg atcgcgtgct gcgcgaatat
      541 ccgcagggaa aggcgcgcct cgataccttg tatcaacttg gcgaagtaga atatcagtct
      601 ggaaactatg cgggcgctct cgaagctttt cgtacgcttg gggcggccgg cggcgaagtc
      661 tcggctcagg cgcagtttcg ggccggtgaa gtgctgatga aggctggtcg atacactgat
      721 gccatcaagg atttcgaaac ctatctcgcc aacttcggtt ccgcgcctct cgtcgacgac
      781 gctcgcttca agatcgggtt gtgctacctt gaactgaaag atcagggcaa ggcgctggcg
      841 gccttttctc agctgcgtga gagcaaaggg tacttccgtc aggaagcccg ctttcagatc
      901 ggcgaaattg cgcgcggtct cggcaactac ccgttggcga ttcagcagta tcaggccatt
      961 ctggccgaag agcccaagca ccccctggct tcacgcacgc gtcgcgccat cgggatctgc
     1021 ctgcatctgg ccaaagacta cccagccgcg gcgacgatgt ttctcgacat tctgcgcgag
     1081 tatcccgcaa ccgatgttgc ggttcccgag agccggttct ggctgggaaa aaacctgatc
     1141 gcgcaggggc gcgtcgaaga cggaattctc gaactgctca aggttccggt gttgtatccg
     1201 aagagccccc tggtcaccga agcctatgcc gaggcggctc gcgcctatca atcacagggt
     1261 ctcacgcagc gagccgaaaa gatgtggcgt gaactgctca aggtgaacaa caaggggccg
     1321 cttgccgaag aggcccgggg ccatctgaaa aaaccctgac cttcgatccc ctcgattttc
     1381 tcgagtctct cctgaccgcc atctggcggt ctttttcttg tccgcgctcc gccatcccga
     1441 aaagcggcat caggaatgga ttctcaggtg tcggctcgat tgtgccgata gggataccag
     1501 aatcattcta cgcagcggag gcagactatg cgcgcaattc ggaaaacggc tctctgggcg
     1561 ccgttgtggg cggctttcct gctcgtcccc ctggttgagg taaccgccaa agacacctac
     1621 gatctcggca aagtgcaggt cgtcggacag gacgcgcagt ccaccagcat gactcccgac
     1681 tccgacacga ttagccagga gatgggtgag cgttcagagc tgacgccgga aattcctctg
     1741 accgaggaaa aggaagtcga aaagcccgtg cagaaaccga ccgcgccgga aacggtcaaa
     1801 acccgcaccg gtggcttcac catgggcatg gcaaaaggaa cccgcaactc gttcgaagtc
     1861 tacggtaagg gtcatggata ccagaacggg tacatcgggt cactctcggt cggacgctcg
     1921 tcgcgcgacg ggtttcgcag cacccgctcc gaagaccgca cctgggccga tctttcgttg
     1981 catggcaacg aaggcagcta cgatctcgac gtggcgggca cgctggccac cgataagttc
     2041 gcgcagcggg gctggcgcac gaatccatct ccggatgccg ccattgaaga tgcgccacgt
     2101 cgtctgatcg tgaaaggctc ttccacgctc cccgatggag cctactttca ggcagaagcc
     2161 gcggtcgact cgatcacgcg gaccacgtcc aaccgcccgt tcggcgtgaa cgaagaagcg
     2221 acgctgtctt ccggccgcgt tggcgcggaa tacgcgagca agctgtcaga ggcggtactc
     2281 ggcaaagtcg ggttcgacat tcgtcgcgac agttttggcc tgcagaacgg ccccgaacag
     2341 gatcttacga agcgcaacct gcgcctgtcg gctcagcatc gcttcgaacg cggctcatcg
     2401 atcgagcttg gtctcaagtc tctcgggttg atgaatcgcg agcgcaccgc gccgatgttc
     2461 cgcctcgact atcgcatgaa agagccctgg cagctgattc tcagctacga cgaagatctc
     2521 ggcaacgact cgctcgaaaa ggtgtttctg ccgcgccagt atgtcgggtt cgccacgctg
     2581 cgggcttctc atcagaaacg taccagcgct aaggtgaact atcgcagtcc tgaacagttt
     2641 caccttggtc tcgaactgtt tcaggaaagt gaagatcagg gcgtcgagta tgtcgacgcg
     2701 catgatgtcg cgcgcaatct gttgacctcg cagattcggt tcgccgatga cgcgaaacgg
     2761 cgtggcatga aggtctctgg gtcggtgaaa ttggacgaac acttcgtgct gaaagccggt
     2821 gcgacgtttc agaacccgga agacaccgcg actggtcgcc agctttccta tgaagcgaaa
     2881 cggttgttcg atgtcgaact ggcgtatgaa caccgctctc tcaagttcaa catcaaccgt
     2941 aagtccgagt cggatcgcgt ggcgtatacc ccggtgacca aggtcggtct cgaggattac
     3001 tcgcgcaccg acctcgaatg caagtatcag tttgacaagc acttttccgc gtttgtccgg
     3061 gttaatgatc tgtatgatga agcacaggaa aaacgttaca acgttcctga agaggggcgt
     3121 gtcagtctgc tcggtgttga agctcagttc taattattat gtttcacatg aatcatcagt
     3181 cgatgcaaag caatgttcga tttcatgtga caggtacaaa tcgtaaatca actctcagcc
     3241 cgtgcgtcgg gcacaaggag gcaagtcatg ggtagcggat ggttcttcgt ggatgtgttc
     3301 gtgaaaggcg ggccggtgat gatcccgatc ctgctctgct ccatcgtcgc cctggccatc
     3361 gtcattcagc gctatcgatt ctttcagagc atcaccttga gcggtgaggg attcatgcgc
     3421 cgcgtgaaat ccgcgctcag cggccgtgac tacgtcgaag ccatga
//
LOCUS       DAORHD010000423         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30459_length_3460_cov_3.670778, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000423 DAORHD010000000
VERSION     DAORHD010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30459_length_3460_cov_3.670778"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKO06_09850"
     CDS             complement(<1..672)
                     /locus_tag="PKO06_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate synthase"
                     /protein_id="HNV69991.1"
                     /translation="MTKRPEYVLLDRNTRAIIFGMQTNAVQRMLDFDYCCKRDKPSVA
                     AIVDPGRREMHKCFYGKKEVFIPIYPDIAAACEENPDIDAMINFASFRSAYETTLQAL
                     EEKQLKTVVVIAEGIPERYSRHMRAVALKKGKWIIGPATVGGIVAGQFKVGNTGGTID
                     NIINSKLHRPGSVGFVSKSGGMSNEAYNIIARNTDGVYEGIAIGGDAYAGSNLLEHLL
                     RFEANP"
     gene            complement(692..1963)
                     /locus_tag="PKO06_09855"
     CDS             complement(692..1963)
                     /locus_tag="PKO06_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP citrate lyase citrate-binding
                     domain-containing protein"
                     /protein_id="HNV69992.1"
                     /translation="MAQRGIREYHAKRMMARHLPTFLGKSIGYDGKIVLVDATTDWKT
                     LLKDNPWLKKEPLVAKPDQLFGKRGKHGLIFANKNLDETRAWIEERMGKATKVGEITG
                     TLTHFLIEPFVKHEAEYYIAIKTQAEGDVMYFSLEGGINVEENWDKVVQIQVPILEGI
                     DGINLEKQLPKQLGENKALVADFLTGLYKYFAAMHFSYLEINPFAIAGKDIVPLDMVA
                     KLDDTAEFCCNEFWGDDMEFPAAFGRTLSPEEERVRELDSLSGASLKLTLLNPEGRIW
                     NMVAGGGASVIFADTVCDLGAAKELAFYGEYSGDPSTQLTYEYAKVVFDLMTRKPDPQ
                     GRPKNLLIGGGIANFTDVANTFTGIIMALKEYAAKLKKVNTRIFVRRGGPNYHQGLAK
                     IKAAAEELGLPIQVHGPEYHMTRIVRDALEQ"
     gene            complement(2131..>3460)
                     /locus_tag="PKO06_09860"
     CDS             complement(2131..>3460)
                     /locus_tag="PKO06_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69993.1"
                     /translation="PLYSKIAPSNMEAMSIGERIRAILGNTKSVISSGMSQYRGQVAE
                     WNQAHGIGPDATFEQELGVGLNQLGAKVGEMPLGGKGNIVVEKLKVIITAIKEKLQSL
                     ITLIRAKILAVAQKIGLAKKTDKEKGPAKESTGVLGPEGVQYADTFSGKLSKGIAEGT
                     QNAKTSLKNSFSVTNLAVTTTVAVGTNMAIQMVRGEKPSFKKAVKMVATAEFAGGVVG
                     SALGAAGGQFTSSLVRAFLPGPVGAIAGSLIPVMMASAGSTIGGSLAGDLRNGQFSIS
                     KAIKAIDPVDLVGSSIGSTIGMMLGAPIPIIGPIIGGIAGGFLGSKVAKWVVGMFKGQ
                     NSVSKLFNRGQNVSISQPSGMSVTVGNSANTGVMTAPGGDMYRPAADMGQAAQAPIAG
                     SPELQAAEKAYYETYMKYNKLVEQGKYDDAKKVYTDLQKYSDQYKQLKGR"
BASE COUNT          608 a         1040 c         1014 g          798 t
ORIGIN      
        1 cgggttggct tcgaagcgca gcagatgttc gagcaggttc gaaccggcat acgcgtctcc
       61 gccgatggcg atgccttcat agactccgtc ggtgttgcgg gcaatgatgt tgtaggcctc
      121 attcgacata ccgcccgatt tgctgacgaa cccgaccgag cccgggcgat gcagcttgga
      181 attgatgatg ttatcgatcg ttccgccggt gttgccgacc ttgaactggc cggccacgat
      241 gccgccgacc gtcgcggggc cgatgatcca tttgcccttc ttgagcgcga cggcgcgcat
      301 gtgccgcgaa tagcgctcgg gaatcccctc ggcgatcacg accaccgtct tcagttgctt
      361 ttcttccagc gcctgcaggg tggtttcata ggcgctgcgg aacgaggcga agttgatcat
      421 cgcatcgatg tccgggtttt cttcgcaggc cgcggcgatg tccgggtaga tcggaatgaa
      481 gacttctttc ttgccataga agcatttatg catctcacgg cggcccgggt cgacgatggc
      541 cgccaccgaa ggcttgtcgc gcttgcagca atagtcgaag tcaagcatgc gttgcaccgc
      601 gttggtctgc atgccgaaaa tgatggcgcg ggtgttgcgg tccaacagca cgtattctgg
      661 tcgtttggtc atggctgttt ttccctttcc tttactgttc cagcgcatcg cggacgatgc
      721 gggtcatgtg atactcgggc ccgtgaacct gaatcggcag gcccagttcc tcggccgcgg
      781 ccttgatctt ggcgaggccc tgatgatagt tcggaccgcc ccgacgcacg aagatgcggg
      841 tattcacctt cttcagcttg gcggcatatt ccttgagcgc catgatgatg cccgtgaacg
      901 tgttggctac gtcggtgaag ttggcgatgc cacccccgat caacaggttt ttcggccgcc
      961 cctgtgggtc tggcttgcgg gtcatcagat cgaagacgac tttggcatac tcgtaggtca
     1021 gctgggtcga ggggtcgccg gaatactcgc cataaaatgc gagttccttg gcagcgccga
     1081 gatcgcagac cgtatcggcg aaaatgaccg aagccccgcc gcccgcgacc atgttccaga
     1141 tgcgaccttc ggggttcagc agcgtcagtt tcaacgaagc gcccgacagc gagtccagct
     1201 cgcgcacccg ttcttcctca gggctgagcg ttcgaccgaa cgccgccgga aactccatat
     1261 cgtcgcccca gaattcattg cagcagaatt ccgcggtgtc atcaagtttt gcgaccatat
     1321 cgagtggtac gatatccttg ccggcgatgg cgaaggggtt gatctcgaga tacgaaaagt
     1381 gcatcgccgc gaagtatttg tacaagccgg tcaggaagtc ggcgaccagg gctttgtttt
     1441 cgcccagctg cttgggaagc tgcttttcga gattgattcc gtcgataccc tcgagaatcg
     1501 gtacctgaat ctggacgact ttgtcccagt tctcttcgac gttgatgccg ccctcgagcg
     1561 agaaatacat cacgtctccc tcagcctgag tcttgatggc gatgtagtat tccgcttcgt
     1621 gtttgacgaa cggctcgatc agaaagtggg tcagggtgcc cgtgatttct ccgactttgg
     1681 tggcttttcc catgcgctct tcgatccagg cgcgcgtttc gtccagattc ttgttggcga
     1741 agatcaatcc gtgcttgccg cgcttgccga acagctggtc gggcttcgcg acgagcggct
     1801 ccttcttgag ccaggggttg tccttgagca acgtcttcca gtctgtggtt gcgtcgacca
     1861 gcacgatttt tccgtcgtag ccgatgctct tcccgagaaa ggtgggaaga tgacgggcca
     1921 tcatacgttt cgcgtgatac tcacgaatgc cgcgttgtgc catgttcaat cactccgtgt
     1981 cttgacaggg ttcgggggct ctgacccccg cggtttcccc gttggtatac ctcgggtcgc
     2041 cgctgctgtc aagcctgaga ggcctatcgc ggcgaaagaa aagcggcgcc cgatcgtcga
     2101 tcgggcgccg cttgagcgcg ttgtcgtcgc ttaacggcct ttcagctgct tgtactggtc
     2161 ggaatacttc tgcaagtcgg tatagacctt cttggcgtca tcgtatttgc cctgctccac
     2221 cagcttgttg tacttcatgt aggtttcgta atacgctttc tcggcagcct gcagttcggg
     2281 cgaacccgcg atcggtgcct gcgcggcttg acccatgtcg gcggccggac gatacatgtc
     2341 gccgccggga gccgtcatca cgcccgtgtt ggcgctgttg cccacggtga cgctcatgcc
     2401 ggaaggctgg ctgatgctga cgttctggcc gcggttgaac agcttgctga cgctgttttg
     2461 gcctttgaac atgccgacca cccacttggc gaccttgctg cccaggaagc cgcccgcgat
     2521 accaccgatg atgggcccga tgatcggaat cggcgcgccc agcatcatac cgatggtcga
     2581 accgatcgat gacccgacca gatcaaccgg atcgatcgcc ttgatcgcct tggaaatcga
     2641 gaactgaccg ttgcgcagat caccggccag actgccgccg atcgtgctgc ccgcgctggc
     2701 catcatgacc ggaatcagcg agccggcaat ggcgcccacc gggccgggca ggaacgcgcg
     2761 caccagcgaa gaggtgaact ggccacccgc cgcgcccagg gctgaaccga ccacaccacc
     2821 cgcgaattcc gcggtggcga ccattttgac tgccttcttg aacgacggct tctcgccgcg
     2881 caccatctga atggccatgt tcgtgccgac ggcgaccgtc gtggtcacgg ccagattggt
     2941 gacggagaag ctgttcttca acgaggtctt ggcgttctgg gtgccttcgg caatgccctt
     3001 cgagagcttg cccgaaaacg tatcggcata ctgaacccct tcggggccga gcacgccagt
     3061 cgattctttg gcgggccctt tctctttgtc agtcttcttc gccagaccga tcttctgagc
     3121 cacagccaga atcttcgcgc gaatcagagt gatcaggctc tgcagctttt ccttgatggc
     3181 ggtgatgatg accttgagct tctcgacgac gatgttgccc ttgccgccga ggggcatttc
     3241 gccgaccttg gcaccaagct ggttcaaccc gacgcccagt tcctgctcga aagtcgcgtc
     3301 aggcccgatg ccatgcgcct gattccattc ggcgacctga ccgcgatact ggctcatgcc
     3361 gctcgagatc accgacttcg tgttgccgag aatcgcgcga atccgttcgc cgatgctcat
     3421 cgcttccatg ttgctcggag cgattttgct gtacaaaggc
//
LOCUS       DAORHD010000424         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30540_length_3453_cov_4.007652, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000424 DAORHD010000000
VERSION     DAORHD010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30540_length_3453_cov_4.007652"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2182
                     /locus_tag="PKO06_09865"
     CDS             <1..2182
                     /locus_tag="PKO06_09865"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HNV69994.1"
                     /translation="ANLLKLSHADPVFPALFLESLRQADSEGLVWPNQALVQWKGGDE
                     AIEARLYRAPVTVVFWGGSEAQAAIRRRQGPETRLIENGPRYSFAVTDRESLSGVAAE
                     NALAGLALDLCRWDQQACSSPHVVYVIDQDDGAADRLLERLAAVLDPLAKSLPRGRLT
                     FDEQVEIRRLRELAGFAALDDAGSVVAPAKFDYTLIREASPDFKVSCLNRTLFCKRVS
                     SVDALIEQIEPMRRYLQTVGLFVSEGTRQKLEPRLLTVGAKRLTGWGGMSEGRDGAPH
                     EGEFLLRRMVDWVDREYAPKSAHRSFSDSQMSGGLLNSDLETRRAEALLEALRRTEYY
                     RPLLLRTSGAPRERLQQLPLLDRETFYRVSPPASTDILSGPLTNAYVYASGGTTGEPK
                     FTFYANDEYRAATDVLALIYEAAGISRRDVVGNLFLAGNLWTSFNVANRALENIGCLN
                     LPIGGASDFGNIIKYLKTFSATAVVGLPSIIVKLAEEVRDRGERLSLKTILYGGEHLR
                     APTIAFLREHLGCELVRSAGYACVDTGPIGYQCLHLEGTLHHVLSGYQMVEILDPQTG
                     EPCPLGQPGEIVATNLERRLMPVVRYRTGDLGRWVEKTTCLCGFGGRTFELLGRCDDL
                     LVIGGINLLPADVAPGVGRLPVSPNFQIIARLVHGREQLTLRLEADAPVSDEIVRQAL
                     VEGSYKLEEALREGWLSLHFEWYKPGQLPRNPRTGKIKVVVDER"
     gene            complement(2194..3417)
                     /locus_tag="PKO06_09870"
     CDS             complement(2194..3417)
                     /locus_tag="PKO06_09870"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNV69995.1"
                     /translation="MVRRNQNFDRFKAQYLFKEIARRRREYAEAHPQARIISLGIGDT
                     TEPLTSHIVQQVQQTAHEMGTREGYRGYGDEPGLHDLRQAISATIYGGVLTADEIFVS
                     DGCKCDIGRLQILFGPEARITVQDPSYPAYVDSAVMMGMTGSLNEQTHQFDGITYLPC
                     RPENQFFPDLSRIPERAIVYFCSPNNPTGAVATREQLGQLVAEVKAKHGLIVFDAAYS
                     AFIRQEHLPRTIYEIPGAREVAIETSSFSKPAGFTGVRLGWTVVPKTLQFEDGSLVWN
                     DFHRLVTTIFNGASIFAQTAGISALSTAGLAETRSQIHYYMTNAQIIRKALVDCGFEV
                     FGGDNAPYIWARIPGKDSWSAFDDLLQRAQVVCTPGAGFGPAGEGFLRFSAFSHRENV
                     EEAVLRLAKVFHSGT"
BASE COUNT          640 a          951 c         1072 g          790 t
ORIGIN      
        1 tgcgaatctg ctgaagctgt cgcatgctga tccggtgttt ccagcgctgt ttctcgagag
       61 cctgcgccag gccgattccg agggcttggt ctggccaaac caggctctgg tgcaatggaa
      121 aggcggagat gaggcgatcg aggctcggct gtatcgtgcg ccggtcaccg tcgttttttg
      181 gggtggatca gaggcgcagg cggcgattcg ccgccgtcag gggcctgaaa cccgattgat
      241 cgaaaacggt ccccggtact cgttcgccgt caccgatcgt gagagcctgt ccggggtagc
      301 ggccgagaac gctctcgccg gacttgctct ggatctctgt cggtgggatc aacaggcctg
      361 ctcttcaccg cacgtggttt atgtgatcga ccaggatgat ggtgccgcag accgtttgtt
      421 ggagcgactt gccgcggttc ttgatccgct ggccaagagt ctgccgcggg gccggttgac
      481 gttcgatgag caggtcgaga ttcgccggtt gcgtgaactg gctggttttg ccgctctgga
      541 tgacgccggt tcggtggttg caccggcaaa attcgattac acgctcattc gcgaggcatc
      601 acctgatttc aaagtgtcgt gtctcaaccg cacgcttttc tgcaagcggg tgtcatcggt
      661 cgatgccttg atcgaacaga tcgagccgat gcggcgctac ctgcagacgg tcggtctgtt
      721 cgtgagcgag ggtacgcgcc agaaactcga acctcgtctg ctcaccgtcg gtgccaagcg
      781 actgaccggg tggggtggca tgagtgaagg acgcgacggc gctccgcacg aaggcgaatt
      841 cctgttgcgt cggatggttg actgggttga tcgtgagtat gcgccgaagt cggcacatcg
      901 gtctttttcc gacagtcaga tgtctggtgg tttgttgaac tcggatctgg agacgagaag
      961 ggctgaagcg cttctcgaag cacttcgccg cacggaatac tatcgcccgc ttttgctgcg
     1021 taccagcggt gctcctcgcg aacgccttca acagctgccg ttgcttgatc gagaaacgtt
     1081 ttatcgtgtc tctccgcccg cgtcgaccga tattctcagc ggtccgctga ccaacgccta
     1141 cgtgtatgcc tcgggcggca ccacgggtga gccgaaattc acgttctatg ccaacgacga
     1201 gtaccgcgcg gccactgatg ttcttgcctt gatctacgaa gccgccggta tcagtcgtcg
     1261 tgatgttgtc ggcaaccttt ttctcgccgg caacctctgg accagcttca atgttgccaa
     1321 tcgtgccctc gaaaacattg gctgtctcaa tcttccgatt ggtggcgcct cggacttcgg
     1381 caatatcatc aagtatctca agactttttc cgcgacggct gtggtggggc tgccttcgat
     1441 tatcgtcaag ttagccgaag aggtacgcga ccgcggtgag agactctcac tgaaaacgat
     1501 cttgtacgga ggcgagcatc tgcgcgcgcc cacgattgcc tttctcagag agcatcttgg
     1561 ctgcgaactg gtgcgatctg ccgggtatgc ctgtgtcgat accggtccga tcggctatca
     1621 atgtctgcat ctcgagggaa cgttgcatca tgtactcagc ggctaccaga tggttgagat
     1681 tctcgacccc cagacgggcg agccttgtcc cctcggccag ccaggcgaga ttgtcgccac
     1741 gaatctggaa cgacgattga tgccggtggt gcgttatcgt accggtgacc tgggcaggtg
     1801 ggtcgagaaa acgacgtgtc tctgtggctt tggaggccgc acgttcgaac tgctcgggcg
     1861 gtgtgatgac ctcttggtca ttggcggaat caacctgctg cctgccgatg tggcacctgg
     1921 agtcggtcgc cttccggtca gcccgaactt tcagatcatc gctcgattgg tccacggtcg
     1981 tgagcagttg acgctgaggc tcgaggcgga tgctcccgtg tctgacgaga tcgtgagaca
     2041 ggcgttggtc gaaggttcat acaaactcga ggaagcattg cgcgaaggct ggttaagtct
     2101 gcatttcgaa tggtataaac ccggacaatt gccgcgaaat ccgcgaaccg gcaagatcaa
     2161 ggttgttgtt gacgaacgct gattttcgtc gggttacgtg cccgaatgga agactttagc
     2221 cagtcgcagc accgcttctt cgacgttttc ccggtgcgag aacgcgctga atcgcagaaa
     2281 accttcacct gccggtccga accccgctcc gggcgtgcag accacctggg ctcgctgcag
     2341 cagatcatcg aaggccgacc aggaatcttt ccccgggatg cgggcccaga tatacggggc
     2401 gttgtcgccg ccgaacacct cgaaaccgca gtcgacgagg gctttgcgaa tgatttgggc
     2461 attcgtcatg tagtagtgaa tctggctgcg ggtttcggcg agtccggccg tcgagagtgc
     2521 gctgataccg gccgtctggg caaagatcga ggctccgttg aaaatcgtgg tgacgagccg
     2581 atgaaagtcg ttccagacca ggctgccgtc ttcgaattgc agggttttcg gtacgaccgt
     2641 ccagcccagg cgcacgccgg tgaatcctgc cggtttcgag aacgagctgg tctcgatggc
     2701 gacctcgcgg gctccgggaa tctcgtagat ggtgcgcggc aggtgctcct gcctgataaa
     2761 ggcgctgtag gcagcatcaa acacaatcag tccgtgcttg gccttgactt ccgctaccag
     2821 ttgtccgagt tgttctcgtg tggctaccgc tcctgtcggg ttattgggtg agcagaaata
     2881 gacgatagcg cgttccggaa tgcgggaaag atcggggaaa aactggttct cgggtctgca
     2941 cggcagatac gtaatgccgt cgaactgatg cgtttgctcg ttgagacttc cggtcatgcc
     3001 catcataacg gcgctgtcaa cataggctgg gtaggacgga tcctgaacgg tgatgcgcgc
     3061 ttccggacca aacagaatct gcaggcgtcc gatgtcgcac ttgcagccat ctgacacgaa
     3121 gatctcgtcc gcggtgagaa ccccgccgta gatcgttgct gaaatggcct ggcgcagatc
     3181 atgaaggccg ggttcgtcac catagccgcg gtatccctcg cgagtaccca tctcgtgcgc
     3241 cgtctgttgc acctgttgca cgatgtgcga cgtcagcggt tccgtggtgt ctccgatgcc
     3301 caggctgatg atgcgtgcct gcgggtgagc ttcagcatat tcacgacgtc gtcgagcgat
     3361 ttccttgaag agatactgcg ccttgaatct gtcgaagttc tgattgcgtc gcaccatgag
     3421 tgtgcctccg ggtcgggatg agggacttga gga
//
LOCUS       DAORHD010000425         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30554_length_3452_cov_4.187518, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000425 DAORHD010000000
VERSION     DAORHD010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30554_length_3452_cov_4.187518"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..310)
                     /gene="rpsT"
                     /locus_tag="PKO06_09875"
     CDS             complement(17..310)
                     /gene="rpsT"
                     /locus_tag="PKO06_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNV69996.1"
                     /translation="MANTNSAAKNARKAARRHARRVAVKSELKTIRKKTIDAARTGSS
                     KDVVKDLATDAIRKFAKAGSNGYIHRRTAARKIGRLMRQLHKLQNQAPAATKK"
     gene            537..3308
                     /locus_tag="PKO06_09880"
     CDS             537..3308
                     /locus_tag="PKO06_09880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV69997.1"
                     /translation="MKRVFVEALQRTGELPEIRSWQTPDFRFLHSLPGFVGWAVRLLQ
                     TEPVQDRVFVLHEPFSQGELLALINWADQLNVLFDADDGGDEVYNCVLPDGDKAMILF
                     APFIQGHVNYGEVMFVARGLTPALYAEWCLFCDHFASLLLSRFLPASRFPATRTGVDP
                     VATLEFVLDLHRLAQWRLTGQDWSPRHPEDQWFEEDAAYLYRLLANFQAQFDLDTVFV
                     VQRVAPELNHIILLSWVSEVEALHPGVREMLEDLLSHDQAGTLADARIKVFQAGRLPP
                     DYQQRTPSSQSFVCEVGEMRYGYLGVCMQREADLKPIYRLMALLANHLAFRFAHLYQL
                     RKEEVFAQKLKNINMACDVLTIAENPRAIFDQLQEHLHRIFGQNDGLILLIPPGTQSP
                     EIERKFGSVPDGVDLAALLGENGWVGEQILEGAALRDISGTHDSIRYIFPFSSTPQVL
                     PESMEIVPQQSLGGLILFHSQRNRDLRDDDHELLQILLDRVKTSLQATFNYQEKLETI
                     KALEGLIGTLMLQDEDQLLGEMIQIIRRLLKVNRVSFLALDDDGAHFRIRKSYGIPLD
                     VVERTRIPLGSEITGTVAMTGRSLKIKNIEESEEFQKRSQERYFNGSLLSVPLKPISS
                     GGTSQVKWVINVNNKINGLIFTDQDQKLLEAIANLIMVALANKELLGAKMEKDLMDRQ
                     MADAREVWSAMLPKSFKNLPEVFSLSAKSEPAREVGGDFYDVLHMTNGRWLLAMGDVS
                     GKGMPAAILMATTRVMLRSAIRDSQEPAAILTHLDKLLAQELDGYHMVTLLLVSVDPG
                     TGQADFASAGHGPVMACLGGQVQEIGPGRGQPLGLGIEPAYFSQNSFQLQSGDLLFLY
                     TDGLTEERAPDREQYGTDRLMTYLRKHQKRQVLELVDGIFREVAQWRQHAEAHDDLTV
                     MAMRYRRNA"
BASE COUNT          672 a          966 c         1040 g          774 t
ORIGIN      
        1 cgtatcagaa aagggcttac ttcttggtcg cagcgggagc ctgattctgc agcttgtgca
       61 gctggcgcat gaggcgaccg attttgcgcg cggccgtgcg gcgatggatg tagccgttgg
      121 aaccggcctt ggcgaacttg cggatcgcgt cggtggccag gtctttcacg acgtctttcg
      181 agctgcccgt gcgggcggcg tcgatggtct tcttgcggat cgtcttcaat tcagacttga
      241 ccgccacgcg ccgggcgtga cgccgggcag ccttgcgagc gttcttggcg gcagaattgg
      301 tattagccat atgttttcac cttccccacg aaatcttaag agttagaggt gtatcctaac
      361 acgtacacac cgatttggca agcttttctt ttttgtcgtt gagcgcgtga actgccggtg
      421 caaggtgagg agaaggttga atcaacctgc cacatccatt ataatgacgc cgttttgtcg
      481 ctgattcgac ttgtttcggg cttgccccgg acaggtaccc cagggaggaa gaacacgtga
      541 aacgggtttt tgtcgaagcg ttgcaacgca cgggcgagct ccccgaaatc cgctcctggc
      601 agactcctga cttccgtttt ctccattccc tgcccggctt tgtcggatgg gcggtgcgcc
      661 tgctgcagac cgagccggta caagaccgtg tcttcgttct gcacgaaccg ttttcgcagg
      721 gcgagctgct ggctctgatc aactgggcgg atcagctgaa tgtgctgttt gatgccgatg
      781 acggcggaga cgaggtttac aactgcgtcc tgcccgacgg ggacaaggcg atgattctgt
      841 ttgccccgtt cattcagggc cacgtgaact acggggaagt gatgtttgtc gctcgcggcc
      901 tgactccagc gctgtatgcc gaatggtgtc tgttttgcga tcattttgcc tcgctgctgt
      961 tgagccgctt tttgccggca tcgcgctttc cggccacccg taccggcgtc gaccccgtgg
     1021 caaccctcga gtttgttctc gatctgcatc ggctggcgca atggcgcctg accgggcagg
     1081 actggtcgcc ccggcacccc gaagatcaat ggttcgaaga ggacgccgcc tacctctatc
     1141 gtctgctggc caattttcag gcgcagtttg atctcgacac ggtctttgtc gtgcagcggg
     1201 tcgctcccga gctcaaccac atcattctgc tttcgtgggt ctccgaagtc gaggcgttgc
     1261 atcccggagt gcgggagatg ctcgaagacc tgctatcgca cgatcaggcg ggtacgctgg
     1321 cagatgcccg catcaaggtg tttcaggctg gccgcctgcc gcccgactat cagcagcgaa
     1381 ctccctcgtc gcaatcattt gtctgcgagg tcggcgagat gcgatacggc tacctgggcg
     1441 tctgtatgca gcgcgaagcc gatctgaagc cgatctatcg tctgatggca ctgctggcga
     1501 atcatctggc gtttcgattc gcgcatctgt accagttgcg caaagaagaa gtcttcgcgc
     1561 agaagctcaa gaacatcaac atggcctgcg atgttctgac cattgccgag aacccccggg
     1621 cgatctttga tcagctgcag gaacatctgc atcgcatctt cggtcagaat gacgggctga
     1681 ttctgctgat tccgccagga acacagagcc ccgagatcga acgtaagttc gggtcggtcc
     1741 ctgacggggt cgatctggcg gcgctgcttg gcgagaatgg ctgggtcggt gagcagattc
     1801 tggaaggcgc agctctgcgt gacatcagtg gcacgcacga cagtatccgg tacatttttc
     1861 ccttcagttc gaccccgcag gtacttcccg aatccatgga aatcgtcccg cagcagtcgc
     1921 tgggcggcct gattctgttt cacagccagc gaaatcgcga tctgcgcgat gatgatcacg
     1981 aactgctgca gattctgctc gatcgcgtga aaacctcgtt gcaggcgacc tttaactacc
     2041 aggaaaagct cgaaacgatc aaagctctcg aaggcctgat cggtacgctc atgttgcagg
     2101 atgaagatca gctgctgggc gaaatgatac agatcattcg gcgtctgctc aaggtgaatc
     2161 gggtttcgtt cctggctctc gatgatgacg gcgcacattt ccgtatacgt aagtcgtacg
     2221 gaattccgct ggacgtggtg gagcgaaccc gcattccgct cggcagcgag ataacgggta
     2281 cagtggccat gaccgggcgc tccctcaaga tcaagaatat cgaagagagc gaggagtttc
     2341 agaagcgatc tcaggaacgc tatttcaacg gctcgctgct atcggtgccg ctcaagccca
     2401 tctcgagcgg cggcacatct caggtgaaat gggtcattaa cgtcaacaac aagatcaatg
     2461 gcctgatttt caccgaccag gaccagaagc tgctcgaggc gattgccaat ctcatcatgg
     2521 ttgcgttggc caacaaagag ctgctcggcg cgaaaatgga gaaggacctg atggatcggc
     2581 agatggctga cgcgcgcgag gtgtggagcg cgatgctgcc gaaatcgttc aagaatctgc
     2641 ccgaggtttt ttctctgagc gccaagagcg aaccggcccg cgaagtgggc ggcgatttct
     2701 acgatgtgct gcacatgacc aacggacgct ggctgctggc catgggagac gtgtcaggga
     2761 agggaatgcc ggcggccatt ctgatggcga cgacgcgcgt catgttgcgc agcgcgattc
     2821 gcgattctca ggaaccggcg gcgattctga cccatctcga caagttgctg gctcaggaac
     2881 tcgacggcta tcacatggtg actttgctcc tggtgagcgt tgaccctggt acggggcagg
     2941 ccgactttgc ctcggccggg catgggccgg tgatggcgtg cctgggtggc caggtgcagg
     3001 aaatcggccc cggccgcgga cagccgctcg ggctgggcat cgaacccgcg tatttttcgc
     3061 agaactcttt tcagctgcag tctggtgatc tgctgtttct ctataccgac gggttgaccg
     3121 aagaacgggc ccccgatcga gaacagtatg gtaccgatcg actcatgacg tatctcagga
     3181 agcatcagaa acggcaggtg ctcgaactgg tcgacggcat tttccgtgag gttgcgcagt
     3241 ggcgtcagca cgcggaagca cacgatgatc tgaccgtcat ggccatgcgt taccggcgga
     3301 acgcctgacc atgtcttcac agccccgttc ctgtcagcac ttattgcgct ttttcacccg
     3361 gctttccacg cgcacggcga cgatggaaaa cctggaccgc ctgcagcaga tcgtgttgag
     3421 cgagcttctt gatctgatcg acgcggacca tg
//
LOCUS       DAORHD010000426         3442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30718_length_3442_cov_3.124594, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000426 DAORHD010000000
VERSION     DAORHD010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30718_length_3442_cov_3.124594"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PKO06_09885"
     CDS             <1..1110
                     /locus_tag="PKO06_09885"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNV69998.1"
                     /translation="VPEWGGKCYEDDVFEVFVLKRDGTTLKLPEAKEISLPAEGIVIL
                     DRVDCHDTRTGSSVFSSRRSARPYKFVTTFHEGMALVSDSGQTRFGFVQRQNFELTIP
                     LEFHEGRDFHDGVALVRREEQWGIVDQTGMFRALPADAGRMSNLPQADILKHRSENSF
                     TACPARDFREGVAAVTSRRGQWGFVDTTGQWVIPPQFDWAGSFSEGLAAVRVGLQYRY
                     IDRSGKTIIPGPFSCARRFSHGLAPVRLKDVFWGLIDTRGTCIVQPAWASVESCSDGV
                     VPFTVLNGARSCNRAGIYDVETREARVFDHLNFIKTFSEGIAVARRGKYGFIDINGNP
                     VACDELSRRTPNDALRWSEASSFRDGLALVESRHL"
     gene            complement(1183..1761)
                     /locus_tag="PKO06_09890"
     CDS             complement(1183..1761)
                     /locus_tag="PKO06_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV69999.1"
                     /translation="MSRFPLMESPLFHLFKGIKAFWWAHRRAEFLAFILALLISLTGL
                     VTAQAAESPPPIELLHEMQPWPSEFGPLTAGFLDPAIGESVQVQWHEHIASVAQRLTP
                     ESLIDISRWAQSEMATCTISLEQIASLSFGPEAQTPNREQVLYSQTVQDGIPLPSHHP
                     LVFRRLLVAVSFDTATRRICRIFVTIRGWREE"
     gene            complement(1778..>3442)
                     /locus_tag="PKO06_09895"
     CDS             complement(1778..>3442)
                     /locus_tag="PKO06_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70000.1"
                     /translation="GTMFIRYMTQERAQTARQSDSIIAYYLAEAGVEKAMVRMRELFS
                     EKLLNPGNEINDNLLSLLDIDRAENFNLKFAIPDGDLLKNGRAEVEIEIMHLKQTPFK
                     SYIDEYVEVPPQLKIYRKENRDTYADKSLGGWEGHIRFVSTGYFKNTKKRIEVMRDLR
                     CADLTPPAENYTLFVSGKKDEYLKEGEFRLRNWSVVRSLKDMVDGIIKAVNDAVMATL
                     GNTAEALFWEPKAASLISFEGDLKVKTLKVIRKLVMSTTDVKIKDYVDTTIQNLHPYL
                     WGKIRTNGRLHAYLPFFAADDIINYFEDNSIFSHQRPEIGYLFCNNQLHDPYLSKYTY
                     YEGEVIKYYQKLKPYILGVTETPYPSSDPYTINTKFDFVSRYPQKLQPLQLERIKKHA
                     KEFAHEYYEGDLTLKGSYAKPAHIMGIIFVQGDVYIGGRVSGQGMVVSEGNIFITDNV
                     IHDSAGAFLSLVSLKGHVAFAKGISAAKVEAAIYAFNSIKGGEQISILGNLCVENLNR
                     MEKEDGPVMMPKRVFINYDADLKSQTGNNVCFNVSRQVVSFRDLGL"
BASE COUNT          688 a          884 c         1050 g          820 t
ORIGIN      
        1 gtgccggaat ggggcggcaa gtgctacgag gatgatgtct tcgaggtgtt cgtgctgaaa
       61 cgcgacggca ccacgctcaa gcttcctgaa gcaaaggaaa tttctctgcc ggcagaagga
      121 atcgtcattc tcgatcgggt tgactgccat gacaccagaa cagggtcgtc ggtgttcagt
      181 tcccggagat cggcgcgacc gtacaagttt gtcaccacct ttcatgaggg aatggcactg
      241 gtcagcgatt ccgggcagac ccgctttggc tttgtccaac gtcagaattt tgaactgacg
      301 attccactgg aattccacga agggcgtgat ttccacgacg gagttgctct ggttcgtcgt
      361 gaggaacagt ggggaattgt cgatcagacc ggtatgttcc gagcgttgcc tgctgatgcg
      421 ggccggatgt cgaacctgcc acaggccgac attctgaagc atcgatctga aaacagcttt
      481 accgcctgtc cggcccgtga tttccgcgaa ggggtcgcgg cggtcacctc tcgccgcggc
      541 cagtggggct ttgtcgatac aaccggccaa tgggtcattc cgccgcagtt cgactgggct
      601 ggcagttttt cggaaggtct ggctgccgtc cgtgtcgggt tgcagtaccg atacattgac
      661 cgctcaggaa aaacgattat tccaggccct ttctcctgtg cccgacggtt ttctcatggt
      721 ttggcacctg tacgattgaa agacgtgttc tgggggctga ttgatacccg gggaacctgt
      781 atcgtccagc cagcttgggc cagcgtggaa tcatgttctg acggggtggt gccgttcacc
      841 gttctgaacg gtgcccggtc gtgcaatcgt gccgggatct acgacgtcga gactcgagag
      901 gcgcgagtct ttgaccacct gaatttcatc aaaaccttct ccgaggggat cgcggtggcg
      961 cggcgcggaa agtacggttt tatcgatatc aatgggaacc ctgtggcgtg cgacgagttg
     1021 agtcgtcgca caccgaacga tgccctgcgc tggagtgaag cttcatcgtt tcgcgatggt
     1081 ctggctctgg ttgaaagccg ccatctgtag atgacggtgt ccggcgagtg cgggtgaata
     1141 caccgattcg agcagagtga cggtggcgtg ggagcggaaa gatcactctt cgcgccagcc
     1201 gcggatggtg acgaagatgc ggcagatgcg ccgtgtcgcc gtatcgaaag acaccgccac
     1261 gagcagacgg cgaaaaacga gcgggtgatg agacggaaga ggaattccat cctggactgt
     1321 ctgcgagtaa agtacttgct cacgattcgg ggtctgggct tccgggccga agctgaggct
     1381 ggcgatctgt tcgagagaga tagtgcaggt cgccatctcg ctctgtgccc atcgggaaat
     1441 atcgatcagt gattcagggg tcagccgttg ggccacagat gcgatatgtt cgtgccattg
     1501 aacttgtacg gattctccaa ttgccgggtc gagaaaaccg gcggtcagcg gtccaaactc
     1561 gctcggccag ggctgcatct cgtggagcag ctcgatcggg ggcgggcttt cagcggcttg
     1621 agctgtgacg aggccggtga gggatatcag gagagcgagg atgaacgcaa ggaattcagc
     1681 tctcctgtgt gcccaccaga atgccttgat gcctttgaac agatggaaca atggcgattc
     1741 catgagtgga aaacgggaca tgcggttgcg gtgccgacta gagcccgagg tcgcggaaac
     1801 tgaccacctg gcgcgacacg ttgaaacaga cgttattgcc ggtctggctc ttgaggtcgg
     1861 catcgtaatt gatgaagaca cgcttgggca tcatcaccgg accgtcttct ttttccatgc
     1921 gattcaggtt ctcaacgcag agattcccga gaatcgagat ctgttccccg cccttgatgc
     1981 tgttgaaggc atagatggcg gcttccacct tggctgccga aatgcccttg gcgaaggcga
     2041 catgcccttt cagactgacg agagacagaa aagcgccggc actgtcgtga atcacattgt
     2101 cggtgatgaa gatattgcct tcgctgacga ccattccctg cccgctgacc cgaccgccga
     2161 tatacacgtc gccttgtacg aagatgattc ccatgatgtg cgcgggtttt gcgtaactgc
     2221 ctttgagtgt taaatcaccc tcgtaatact cgtgcgcgaa ctccttggcg tgcttcttga
     2281 tgcgttcgag ctgcagcggc tgcagcttct gcggataccg ggagacaaaa tcgaatttgg
     2341 tgttgatggt atacggatca gagcttggat acggcgtttc agtaacaccg aggatgtacg
     2401 gtttcaactt ctggtagtat ttgatgacct cgccctcgta atacgtgtat ttgcttaagt
     2461 acgggtcgtg caactggttg ttgcagaata gatacccgat ttcggggcgt tgatgcgaaa
     2521 aaattgagtt gtcttcgaag tagttgatga tgtcgtcggc ggcgaagaaa ggcagatacg
     2581 catgcagacg cccgttggtt ctgatttttc cccagagata cgggtgcagg ttctgaatcg
     2641 tcgtatcgac gtagtccttg atcttgacgt cggtggtcga catgaccagc ttgcgaatga
     2701 ccttgagcgt tttgaccttc agatcgcctt cgaagctgat gaggctcgcg gctttgggtt
     2761 cccagaacag ggcttcggcg gtgttgccga gcgtcgccat aaccgcgtcg ttgaccgcct
     2821 tgatgatgcc gtcgaccatg tcctttagtg aacggaccac gctccagttg cgcagtcgaa
     2881 actcgccttc cttgaggtat tcgtccttct tgcccgatac gaacagcgtg tagttttcgg
     2941 cgggaggggt cagatcggca caccgcagat cgcgcatcac ttcaatgcgt ttcttcgtgt
     3001 tcttgaagta tccggtgctg acgaagcgga tgtggccttc ccagccgccg agcgacttgt
     3061 ccgcgtacgt gtcgcgattt tccttgcggt agatcttgag ctgcgggggg acttcgacat
     3121 actcgtcgat gtacgacttg aacggggtct gtttgaggtg catgatctcg atctcgacct
     3181 cggcccgtcc attcttcagc aggtcgccgt caggaatcgc gaacttgagg ttgaagttct
     3241 cggcccggtc gatatccagc aaagacagca ggttgtcgtt gatctcgttg ccgggattga
     3301 gcaacttctc ggaaaacagc tcgcgcatgc gtaccatcgc cttttccacc ccggcttcgg
     3361 cgaggtagta ggcgatgatc gaatcgctct gccgtgcggt ctgggcgcgt tcctgggtca
     3421 tgtagcgaat gaacatggtg cc
//
LOCUS       DAORHD010000427         3438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30776_length_3438_cov_3.316287, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000427 DAORHD010000000
VERSION     DAORHD010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30776_length_3438_cov_3.316287"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..867
                     /locus_tag="PKO06_09900"
     CDS             55..867
                     /locus_tag="PKO06_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV70001.1"
                     /translation="MPHHESRLDVPPSTFWSKTRGHLPEPRNGRLWRGLVVSDLHLFR
                     EHSQGEAAIVRLRERIEPADFIVLNGDIFDFRWAPHPTLAVTEEAAVDWLRTFVASVG
                     PAVVFYVMGNHDCHESFRQRLMSEPRLFLPGGWFPSFVRVGSALFLHGDLPLFRPLSN
                     CLQRKHGRIPKKRGVMARLWTLGVICPLFGRLAPLVFPKEWSARQVTRSVRRHGPDFR
                     ERLADICFGHIHYPFEDHRLRHLRVHNSGAPLPGFQWQVVSIETADGDWEGA"
     gene            complement(933..>3438)
                     /locus_tag="PKO06_09905"
     CDS             complement(933..>3438)
                     /locus_tag="PKO06_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70002.1"
                     /translation="IAGALCRAVESGRLTTDSHELLVSALEYAPLKQYRLYSGLYKMF
                     EKNPEFLVASVSFMVDRPSLQSQIPRFLQQAVMSETGYPREKLKEILLVFADILGQHE
                     LRTSLVTCCVWYAEAGVDLRGLYAVLDQVITDGKDQERWDAVYAKAFSQLMHEKSYRN
                     PDRLLKELPDGLRGAVLRALTLVAEKRPDRARSLVDRLARELIRPASSLKAVAKELLE
                     QLRRRNMCITPGRAVMNELCEALAQGETELCAFLGSLAERSKTLAKRLLDRLATVSST
                     RTSALEWLQSQCQDCLDETVTPGCSICRVLPRYREYGHELDVPKEVQKLVDVPGGKGS
                     AQACPACGSLYVIRKSEVWESASEGMGVDVTIRVQRLLGEECRGCLPDLSDAEFELWL
                     ETGLQKARRLLRHLEPYLRIDAAWMLAQHALKHHAPDALRELLRNTAGEVRLTALQAM
                     ASIALPERVAFTPELNRLLSDQVVAVRKQAAELLYVMLISQNDHTAIMELANGDPVIA
                     GDVLGKIMNDESLDQSLFLPLYQRGLTSDDVTVRSRSMHALGEAIRRQHDGEKNLELL
                     LKIYDTGDPKVREEVLAVFKGIATCLPPLQVIPRILEVIADSGMIGYYATEIVKILST
                     RSPLPEVLPRLLPLIRNRKQVRREEFITICANLILQHRKSNPSSERYLDVLAEWLSES
                     SESLRRVATATLQTLIEDGVDVTPILEAMLQMLLASNITYVESYFAEPVAAFLARRRD
                     WDNLRRLFAGAKDISGAAVLVLRKHLATVDITPVVRDLAQLLIHPRSYVRENAQQTLA
                     AFGAIGETQKSLVLSTLQECPVEGPEVMKLKQKLAG"
BASE COUNT          694 a         1049 c          941 g          754 t
ORIGIN      
        1 cggcaaaaag cttgtgttgt cgaatgcgtc tggtagcggt agagtatcgg tatcatgccc
       61 caccacgaga gcagactcga cgttccccct tcgacgttct ggtcgaagac tcgcggacac
      121 cttccagaac cgcggaacgg tcgtctctgg cgtgggctcg tcgtctcgga tctgcatctg
      181 tttcgcgagc attcacaggg cgaagcggcg attgtcaggc tgcgcgagcg catcgaaccg
      241 gccgacttca tcgtcctgaa cggggacatc ttcgattttc gctgggcccc ccaccccacg
      301 ctggccgtca ccgaagaggc agccgtcgac tggttgcgaa ctttcgtcgc ctcggttggc
      361 ccagccgtcg tgttctacgt catgggaaat cacgactgtc acgaaagttt tcgtcagcgc
      421 ctgatgagcg agccgcgcct ctttctccct ggtggttggt ttccgtcgtt tgtccgggtc
      481 ggctcagcgt tgtttctgca tggagacctg cctttgttca gacccctttc aaactgtctg
      541 cagcgaaaac acggccgcat tccgaaaaag cgcggagtga tggctcgtct ctggaccctt
      601 ggagtcatct gccctctctt cgggcgactg gccccgttgg tgtttcccaa ggaatggtcg
      661 gcgcgccagg tcacccggtc cgtcagacgt cacggccccg actttcgcga gcgtctggcc
      721 gacatctgct tcggccacat tcattaccca tttgaagacc atcgccttcg ccacttgcgc
      781 gttcacaact cgggtgcgcc cctgcccggc tttcagtggc aggtcgtttc gatcgaaacg
      841 gctgacggtg actgggaagg cgcttagctc accgcctccg tcgttcaggg ctcgccggcc
      901 aaaacggttc gattctgcag cgagtcagtc tctcaaccag ccagcttctg tttcagcttc
      961 attacctcgg gcccctcgac gggacactcc tgtaacgtcg acaagaccag cgacttctgc
     1021 gtctctccga tcgctccaaa tgcggcaagc gtttgctgcg cgttttccct gacgtatgag
     1081 cgcggatgta tcaacagctg cgccagatcg cgaacgaccg gagtgatatc gactgtggcg
     1141 aggtgcttac gcaacaccag tacagccgcg ccactgatgt ctttcgcgcc cgcgaacaga
     1201 cgacgcaggt tgtcccagtc gcgacgtcgg gccagaaagg ctgcgaccgg ttcggcgaag
     1261 tatgattcca catacgtgat attcgaagcc agaagcatct gcagcatggc ttcaaggatg
     1321 ggcgtcacgt ctactccatc ttcgatcaga gtttgcagtg tcgcggttgc cacgcgtcga
     1381 agactctcac tcgactcgga caaccactcg gccagcacgt caagataccg ttcgctcgag
     1441 gggttgcttt tccggtgctg cagaatcagg ttggcgcaaa tggtgatgaa ctcttcacgg
     1501 cgcacctgtt ttcgattgcg tatcaatggc agaagacgcg gcagaacttc aggcagcggt
     1561 gatcgcgtcg atagaatttt cacgatctcg gtcgcgtagt atccaatcat tcctgaatct
     1621 gcaatgactt caagaatccg ggggatcacc tgcaacggtg gcaggcaggt cgcgatcccc
     1681 ttgaacacag cgagcacctc ttcccgtacc ttcggatcgc cggtgtcgta gatcttcaac
     1741 agcagttcga ggttcttttc cccatcgtgc tgtcgacgaa tggcttctcc gagtgcgtgc
     1801 atcgatcgcg aacgaacggt cacgtcatct gaagtgaggc ctcgctgata aagcgggaga
     1861 aacagcgatt gatcaagaga ttcatcgttc atgatcttgc caagaacgtc accggcaatc
     1921 accggatctc cattggccag ttccatgatc gctgtgtgat cattctggct gatcagcatc
     1981 acatagagca gctcggcggc ttgttttcgc acggcgacaa cctgatcaga cagcaatcgg
     2041 ttcagttcgg gggtaaacgc cacccgctca ggcagtgcga tcgaggccat tgcctgcaga
     2101 gccgtcagac gcacctcacc cgccgtgttc ctgagaagtt ctcgcagtgc gtctggcgcg
     2161 tggtgcttca gggcatgttg agccagcatc cacgcggcat cgatgcgcag atagggttcg
     2221 aggtgtcgca gcagacgacg cgccttctgc agaccagttt cgagccacaa ctcaaactcc
     2281 gcatcagaaa gatcgggcag acaaccccgg cattcttctc cgagcaatcg ttgcacgcga
     2341 atggtcacgt cgacgcccat tccttcgctt gccgattccc acacttcact ttttcgaatg
     2401 acatacagac tgccgcatgc agggcaggct tgcgcgctcc ccttcccccc agggacatcg
     2461 accagtttct gcacctcttt gggaacatcc agctcatgtc cgtattcgcg gtatctcggc
     2521 agaacgcgac atatggaaca accaggcgtc accgtttcat caaggcagtc ctggcattga
     2581 ctctgtaacc actcgagcgc agaggtccgc gtggaggaaa cggttgccag gcgatcgagc
     2641 aggcgtttcg ccaaggtttt cgagcgttct gccagcgagc ccagaaacgc gcaaagctcg
     2701 gtttcgccct gcgccagggc ttcgcacagt tcgttcatga ctgcccgacc gggcgtgatg
     2761 cacatgttcc tgcgtcgaag ctgttcgaga agttctttcg ccacggcttt caacgaagag
     2821 gccggacgaa tcagttcgcg tgcaaggcga tcgaccagcg aacgggcccg gtcaggacgt
     2881 ttctcggcga ccagtgtgag ggctctcagc acggcaccgc gcaggccatc aggcagttcc
     2941 ttgagcagcc ggtccgggtt gcgataactc ttttcgtgca tcaattgcga gaatgccttg
     3001 gcatacacgg catcccaccg ctcttgatct tttccgtctg tgatcacctg gtcaagaacg
     3061 gcatacaacc cccgcagatc aaccccggct tcagcgtacc agacacaaca agtcacgaga
     3121 ctcgtccgaa gctcatgctg gccaagtata tctgcgaata cgagcagaat ctctttcagc
     3181 ttctcgcgcg gataccccgt ttcactcatc accgcctgct gcaagaaccg cggaatctgg
     3241 ctctgtaagc tgggacgatc gaccatgaaa gaaaccgatg ccacaagaaa ctcagggttc
     3301 ttttcgaaca tcttgtacaa cccggaatac aaccgatact gtttgagagg tgcgtattcg
     3361 agggctgaca ccaggagctc gtgtgaatcg gtggttaacc gcccgctttc caccgcgcgg
     3421 cacagagcgc cggcgatc
//
LOCUS       DAORHD010000428         3431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30853_length_3431_cov_3.783472, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000428 DAORHD010000000
VERSION     DAORHD010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30853_length_3431_cov_3.783472"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="PKO06_09910"
     CDS             <1..883
                     /locus_tag="PKO06_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70003.1"
                     /translation="LAPMILRNLATIGNINVVPELIKRLKREPLVDLEQCNQSGYESN
                     VSRIAATLAVLGGPTASKALIERSWEAIVRECPYAWKLEELKKRSWSEYYDSKERHQV
                     DTCTDLARALGFCADKAAVKPLVRALISGDEYLTRVAGESLERLTNHHFGFSKLEKPG
                     EPTGREEETSLNVATGAWRVWYEANLHQSYDDWIRNGFAEAGLDVYPASDPTKLKTLI
                     QGLSDLRENVRYNCYMELGSRTGQWFFRDRIRAFHSPVDYCYAAARYRSWLAKRTSPL
                     IWDDKVRCFNETRSSEP"
     gene            complement(896..2497)
                     /locus_tag="PKO06_09915"
     CDS             complement(896..2497)
                     /locus_tag="PKO06_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70004.1"
                     /translation="MNIRRTARWLLLPLGLLLCGCLDTFAADAELLFVEPVARQEGQA
                     VRELTDIRVERVAGGRALSFVPALQTRNQRWKRVEATLQVSPAVREALRLRSLAQTLA
                     PAPAQKWLSARGPLPVWVKARGVGTRGPGLVTVVPVRVAGRETESTHEAHQVLILEDL
                     ALPGGKLVTVEDLSEVGLLVPMVCHEICHGIMAEIYRERFLLLQILSPMPGGQHDSPV
                     ETDPYIAWKEGFAEAGELWLAKQFPNEFQPRLSPVGYAPHVLALAQRMHQRRVKLAER
                     NFYIFAANGQVKDGQLKTGKTDLATEGVIASLLWRIWESAGFDDPLRATLTTLSRHAP
                     ATFFDFCSLLQKDHPTQRDVLKRILLEFTRYTLVSPEALKLYESYYLAKKAYVTKKIS
                     ADDYRKARQTWEDWKTTQRQRIEKGAPLTEAVPQPLIVATRDGYTLDLNDEQVDRLAW
                     HLEAFIPGDDAALKSRQAVRYAESIVEQRRSLGTFTSGTQLADCVPAWLVAKLAAGYR
                     RSQAQVEKALDAELTARRTLRDFVE"
     gene            complement(2514..>3431)
                     /locus_tag="PKO06_09920"
     CDS             complement(2514..>3431)
                     /locus_tag="PKO06_09920"
                     /inference="COORDINATES: protein motif:HMM:NF021943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70005.1"
                     /translation="DLVTLQDHINQINSQIWAGGKKVYKNVTDNFGLEPTGSFNELNL
                     TATMTVLWLAPYQNYAGFFNSADLYSKAYEGNSNECKMVYLYFDGGSEALFNYGSTLA
                     HELQHMINFYQKKLNNKYEEAWLDEAMSGYAEHIAGYSIVSGSNASKARLVRDFLANP
                     QSNDLTSWAGSVSDYGQAYLFGLWLAEKYGSNGSVKTLLSQNAVGIPAVSAFAGGRAF
                     AEIVKNWQLGLYINDTTGDTVYGYKNFSLRNTYSNVTLSGPANAHVGTITVARNAAAY
                     VEVAAPTTATTLTWTVPTTLSVFELNKNW"
BASE COUNT          735 a         1017 c          921 g          758 t
ORIGIN      
        1 actcgcaccc atgattctgc gcaatctcgc gactatcggc aatatcaacg tggttcctga
       61 actcatcaag cgtttgaaga gagagccgct cgtcgatctt gaacaatgca accagtcggg
      121 ctacgagagc aacgtctcac ggatcgcggc aacattggcc gttcttggtg gccccaccgc
      181 ttcgaaggcc ctgatcgaga ggtcatggga agccattgtt cgtgaatgtc cctatgcctg
      241 gaaactcgaa gagctcaaga agcgttcatg gtctgaatac tatgattcaa aggagcggca
      301 ccaggttgac acctgcactg atctggcacg tgcgctcggc ttctgtgccg acaaagccgc
      361 cgtcaagccg ttggtgcgtg cgttgatttc cggtgacgag tatctgacac gggttgccgg
      421 agaaagtctc gagcgcctca cgaatcatca cttcggcttt tccaaactcg aaaagccggg
      481 agaaccgacc ggtcgtgaag aggaaacctc cctcaacgta gccaccggcg cctggcgggt
      541 ctggtatgag gcgaatctcc accagtctta cgacgattgg atccgaaacg gattcgccga
      601 agccggtctc gacgtctatc ccgcgtccga tcccacgaag ttgaaaacgc tgattcaagg
      661 cctgtctgac ctgcgcgaga atgtccgtta caactgttac atggaactgg gatcgcgtac
      721 tggccaatgg ttctttagag acagaatccg tgcgtttcac agcccggtgg attactgcta
      781 cgcggctgcc cgctaccgtt cgtggctggc aaagcgcacc tcccccctga tctgggacga
      841 caaggtgcgt tgtttcaacg agacccgctc gtccgagccg taacgtcgct cctcgttact
      901 caacgaagtc gcgcagagta cgacgcgccg tcagttcggc gtcgagggct ttttccacct
      961 gcgcctgaga gcgacgatac ccggcggcga gtttggcgac cagccaggcc ggcacgcaat
     1021 cagccagctg agtgccggag gtgaaggtgc ccagcgaccg gcgctgttcg acgatgctct
     1081 cggcatagcg taccgcctgc cgcgacttca atgccgcatc atctccggga atgaaggctt
     1141 cgagatgcca ggcgaggcga tcgacctgtt cgtcgttgag atcgagggta tagccgtcac
     1201 gggtcgcgac aatcaacggc tgcggcacgg cttcggtcaa cggtgcccct ttctcgatgc
     1261 gttgccgctg cgtggttttc cagtcttccc aggtctgtcg ggctttgcgg taatcgtcgg
     1321 cactgatctt tttcgtcaca taggctttct tggccagata atacgactcg tacagtttaa
     1381 gcgcttcggg cgagaccagt gtgtaccggg tgaactcaag cagaatccgc ttcaaaacat
     1441 cacgttgggt gggatgatct ttctgcagca acgagcagaa gtcgaagaac gttgccggag
     1501 catgcctgct gagcgtcgtc agcgtggcgc gcagcgggtc gtcgaaccca gcgctttccc
     1561 agatacgcca gagcaagctc gcaatcacgc cttccgtcgc caggtcggtc ttgcctgtct
     1621 tcagctggcc gtctttcacc tggccgttcg cggcaaagat gtagaagttg cgttcggcca
     1681 gcttaacgcg acgctgatgc atgcgctgcg ccagggcgag cacgtgagga gcatagccca
     1741 ccggcgacag gcgaggttga aactcgttcg gaaactgttt cgccaaccag agttcgccgg
     1801 cttccgcaaa gccttctttc caggcaatgt aagggtcggt ttcgaccggc gagtcgtgct
     1861 ggccgcccgg catgggcgat agaatctgca gcagcagaaa acgttctcgg tatatttcgg
     1921 ccatgattcc gtgacagatc tcatgacaga ccatgggcac cagaaggccg acttcgctga
     1981 ggtcttcaac cgtgacgagc ttgccgcccg gcagggccag gtcttcgaga atcagcacct
     2041 gatgtgcttc gtgagtcgat tccgtctcgc gcccggcaac ccgcaccgga acgacggtaa
     2101 ccagacccgg gccacgggtg ccgaccccac gcgctttcac ccacaccggc agcggtccgc
     2161 gggcactcag ccatttctgg gccggtgccg gagccagcgt ctgtgccaga gaccgcagcc
     2221 gcaacgcttc gcgcacagcc gggctcactt gcagggttgc ttcgactctc ttccagcgct
     2281 gatttctcgt ctgcagcgcc gggacaaacg acaacgcccg gcctccggcg acccgctcga
     2341 cccgaatgtc ggtcagctca cgaaccgcct gcccttcctg gcgcgccacc ggctcgacaa
     2401 acagcagctc tgcatctgcg gcgaacgtat cgagacaacc gcacagcaat agtccgagcg
     2461 gcaatagaag ccaacgagct gttcgtcgaa tgttcatggt cgtctcctgc acgttaccag
     2521 ttcttgttga gctcaaacac cgagagcgtc gtcggcacgg tccaggtcaa cgtcgtcgcc
     2581 gtcgtcggtg ccgccacctc aacatacgcg gccgcgttgc gagcaacggt gatcgttccg
     2641 acatgcgcat tcgccggccc actcaaagtg acattgctat acgtatttcg cagactgaag
     2701 ttcttgtaac catacaccgt gtctcccgtc gtatcgttga tgtaaagtcc caactgccaa
     2761 tttttgacaa tctcagcaaa tgcccttcct ccagcaaatg cagatactgc tggaattcca
     2821 acggcattct ggctcagtag tgttttcaca ctaccattgc tcccgtattt ttcagccaac
     2881 cacaaaccaa atagataagc ttgtccgtag tcggaaacgc ttcccgccca gctcgtcaga
     2941 tcgttcgact gtggattcgc gagaaagtcg cggaccagtc gagccttact agcattgctt
     3001 ccagaaacaa tcgaataccc ggcaatatgc tctgcatacc ccgacattgc ctcatccaac
     3061 caagcctctt catatttgtt gttcagcttc ttctgataaa aattgatcat gtgctgtagc
     3121 tcatgagcga gagttgaacc gtagttaaaa agtgcttcag atccaccatc aaaatagaga
     3181 tagaccatct tacactcatt ggaattgccc tcgtaagctt tcgaatacag gtctgccgaa
     3241 ttgaagaaac cagcgtaatt ttggtaaggt gcaagccata acacggtcat tgtcgcggta
     3301 agattcaatt cattgaagct tccggttggc tcgagtccaa aattgtcggt cacatttttg
     3361 tatacttttt ttccacctgc ccaaatttgg ctgttgatct gattgatgtg atcttgcagc
     3421 gtgacaagat c
//
LOCUS       DAORHD010000429         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_30997_length_3419_cov_4.826992, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000429 DAORHD010000000
VERSION     DAORHD010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30997_length_3419_cov_4.826992"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..631)
                     /locus_tag="PKO06_09925"
     CDS             complement(197..631)
                     /locus_tag="PKO06_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70006.1"
                     /translation="MIATMTLDELTIRAEVIAYASVKTTEDAGESEPGMKLMKTHLQI
                     TNVLKGAVKVGDIVEITTVKGMEDEPEFLPKGNYLLLLKKNAAGEYQTVNLIQGAWPV
                     DNNGMFAGLGLGTTPDQLKKSIAATKDKMPPADDDAAPEPEF"
     gene            826..2934
                     /locus_tag="PKO06_09930"
     CDS             826..2934
                     /locus_tag="PKO06_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV70007.1"
                     /translation="MNILQVINALTWGGAQTLLLDLCRYLKSQGHSVTVAAFRDGPLG
                     PSFRQAGIQTLLLEERLFDVLGFWQLSHWIRAQQPDLIHTHLFRASFWARLAARRTTH
                     APVLTSVHGMESAHFQGIEKHTAHWSHCLVFPSHHLKSWYERSIRALPSSRVEIIAPG
                     APVSAELPPKVPRSPLRIGTLSRLHPVKGIDRLLAALCQLKQQRVPFELLIGGDGKDR
                     PRLEALSRDSGMADCVRFIGPVQHAAAFLGSLDLFCAPSREEAFGINVCEAMERGIPT
                     VATDVGGFREIIENGVSGILLPGEQPEQWAAILRPLLVDQRRRALLGQAGREQILRRY
                     DRHTCHTQYLSLMDRLTGETRSDSSLQIAISSSELGGGERLALALATEFHHRGRNVRV
                     LCTGNPLQQKFSAAGIPVETVSLRAGGIFFGARLLRSTRRLSQCLIHAHLNRAALMAG
                     FLDRWLGQPTIAHVHGLNRSVYYRWCRRLIAVSRSVAEHLQSQGIPADQISVLPNAIP
                     PSAPTSSVAPSPPPWRIGIVAKLHANKGHDWALQAIDEARDRLPPLQILIFGDGPERA
                     ALTERWGSGSLKDCLQFFGFQPDMSVYYPTFHLSLLPSLSEGIPLSLLEALSYGIPVV
                     ATRIGGIPEIVEDGVNGFLIPPHDRTALQNALYQVLQPAAHARLSEASKNSFQARNPF
                     SQLVHGVDNLYRVVSDPRHA"
     gene            2931..>3419
                     /locus_tag="PKO06_09935"
     CDS             2931..>3419
                     /locus_tag="PKO06_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70008.1"
                     /translation="MMATTISPFGDHLCFISPCRFDHLRQRHQALANCFAETGTRVTY
                     VNPIGSAGFSLRRQRFGESLQIFDLTVPFRATQHPGLQRITCLAAKRLLQLHGEWSPR
                     NSHLWLADPAWCSWTTAPWQKIYYDRCDRHGFFPRQCREAWLHYELSLYKLSHIIFCT
                     SKV"
BASE COUNT          661 a         1091 c          900 g          767 t
ORIGIN      
        1 gcatccgaga gcgaggtgct gtcggttcgc atgtacgtga tgtagccgtt ctcgatgccg
       61 cgcgcgcagt tcacgcagct catgccgcca atctgaacaa acagttcctg ttcggtcatg
      121 acacctgact attcctgaaa aagtaagaga aacgggcatg ggaaagtgct ttcccatgcc
      181 cgtttgcaat cagtggttag aattcgggtt cgggagccgc gtcatcatcg gccggcggca
      241 tcttgtcttt tgtcgcagca atcgactttt tcagctgatc cggggtcgtt cccaggccca
      301 gtccggcaaa catcccgttg ttatcgactg gccaggctcc ctgaatcagg ttcaccgtct
      361 gatactcacc cgcggcattc tttttcaaga gcaacagata gttgcccttg ggaagaaact
      421 ccggctcgtc ttccatccct ttcaccgttg tgatctccac gatgtcgccg actttcaccg
      481 cgcccttcag aacattcgtg atctgcaggt gcgttttcat cagtttcatt ccgggttcac
      541 tctcacccgc atcttcagta gtcttcacgc tggcgtaggc gatgacctcc gcccgaatcg
      601 tgagttcgtc cagcgtcatc gtcgcgatca tggcataacc tccggttgat acgaaacaca
      661 gcagaaccac gaaccaccaa cagcgaaacc tcgacatacc gtcctccatc tgcgaaaatt
      721 gctcgttcac cgagagaaac gttcccggcg ggcagcggat tcgtcgaatc gtatgtatat
      781 cacagacacg ccaatggtaa catagctctc gaaaaccgtg acgtcatgaa cattcttcaa
      841 gtgatcaatg ccctcacctg gggcggtgcc cagacgctgc tgctcgatct ctgccgctat
      901 ctgaagtcac agggccactc ggtgacagtg gccgcgtttc gcgatgggcc gttgggcccc
      961 tcgtttcggc aagcgggtat tcagacgctg ctgcttgagg agcgactctt cgacgtgctc
     1021 ggcttctggc agttaagcca ctggatccga gcgcagcaac cagacctgat tcacacgcac
     1081 ctgtttcgtg ccagtttctg ggcccgactg gcagcgcgcc gcaccactca tgccccggtt
     1141 ctgacatcgg ttcacggcat ggaatcagca cactttcagg gcattgaaaa gcacaccgcc
     1201 cactggtcgc actgcctcgt ctttcccagc caccatctca agtcctggta tgaacgttcg
     1261 atccgcgccc ttccatcatc gcgcgtcgag atcatcgctc ccggggctcc tgtctccgct
     1321 gagctgcccc caaaagttcc gaggtctccg cttcgcatcg gaacgctctc tcgcctgcac
     1381 ccggtgaagg ggatcgatcg actgctggcc gcactgtgcc agctgaaaca gcagagagtg
     1441 ccgtttgaac tgttgattgg aggcgacggc aaagaccgac ctcgtctcga agccctgagt
     1501 cgggacagcg gcatggccga ctgcgttcga ttcatcggcc ccgtgcagca cgcagcggcg
     1561 tttctcggct ctctcgacct gttctgtgcc ccttcaaggg aagaggcgtt tgggatcaac
     1621 gtctgtgaag ccatggaacg ggggataccc accgtggcga ccgatgtcgg cggttttcgc
     1681 gagatcatcg agaacggcgt ctccggaatc cttctcccgg gtgagcaacc cgaacagtgg
     1741 gcggccattc tgcgtccatt actggtcgat caacgccgac gcgctctgct cggccaggct
     1801 ggacgcgagc agattctgcg gcgatatgat cggcacacct gtcacaccca atatctctcg
     1861 ctcatggacc gcctgacagg agaaacacgt agcgactctt cgctgcagat cgccatttcc
     1921 tcgagcgaat tgggcggagg cgaacgattg gcgctggcac tggccacgga gtttcaccac
     1981 cgcggtcgaa atgtccgagt tctttgcaca ggaaaccctc tgcaacagaa attttccgct
     2041 gccggtattc cggttgaaac agtttctctg cgagcgggcg gaatcttctt cggcgcacga
     2101 ctgctgcgca gcactcgccg actttcccag tgccttattc atgcgcacct aaacagagct
     2161 gctctgatgg ccggttttct cgatcgatgg ctgggacagc caaccatcgc acatgttcac
     2221 gggctcaatc gttcggtgta ttatcgctgg tgccgccgcc tgatcgccgt ctctcgttca
     2281 gtggctgagc atctgcagtc tcagggaatt cccgcagatc agatctccgt gctccctaac
     2341 gccataccac catctgcacc gacgtcttcc gtcgcgccgt ctccgcctcc gtggcgcatc
     2401 ggcatcgtgg ccaaactgca tgccaacaag gggcatgatt gggcattgca ggccatcgat
     2461 gaagcgcgcg atcgtttgcc gccgctgcag atcctgatct tcggcgatgg gccagaacga
     2521 gcggctctca ccgagcgctg gggctccggc tcgctcaagg attgcctgca gttcttcggc
     2581 tttcagcccg atatgtcggt ctactacccc acattccacc tgtccctgtt gccgtcactc
     2641 agcgaaggca ttcccctttc gcttctcgaa gcgctttctt acggcattcc tgtggtggcg
     2701 acacgaattg gtggcatacc ggaaatcgtc gaagatggcg tcaacggatt cctgatcccc
     2761 ccgcacgatc gaacggctct gcagaacgcg ctgtatcagg ttttgcaacc cgcggcacac
     2821 gcgcgcctca gtgaagcctc gaaaaactct ttccaggctc gtaacccgtt ttcacaactg
     2881 gtacacggag tcgacaatct ctaccgggtc gtttctgacc cgcggcacgc atgatggcca
     2941 cgaccatctc tccgttcggc gatcacctgt gtttcatctc gccctgccgc ttcgaccacc
     3001 tgcgacaaag gcaccaggcg ctggcgaact gctttgccga gaccggcacc cgggtcacct
     3061 acgtgaaccc gatcggctcg gcaggcttct cgttgcgacg tcagcgtttc ggcgagtcac
     3121 ttcagatctt cgatctcacc gtgccgttca gagccactca gcacccaggc ctgcaacgca
     3181 ttacgtgcct ggcggcaaaa cgactgctgc aactgcacgg ggaatggtca ccgcgaaaca
     3241 gtcacctctg gctcgcggat ccggcctggt gctcatggac taccgccccc tggcagaaaa
     3301 tttactacga ccgatgcgat cgacatggtt tttttccccg gcagtgtcga gaggcgtggc
     3361 ttcactacga actgtctttg tacaaactct ctcacatcat cttctgcact tcaaaagtg
//
LOCUS       DAORHD010000430         3419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31000_length_3419_cov_3.889715, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000430 DAORHD010000000
VERSION     DAORHD010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31000_length_3419_cov_3.889715"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..1383)
                     /locus_tag="PKO06_09940"
     CDS             complement(82..1383)
                     /locus_tag="PKO06_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV70009.1"
                     /translation="MNVTRYLRHGLIGGMIGACLFSVGVLPVRAQEPDKLRQARRTID
                     QLQEELDSVKVQIDFLDLKVRDSRDHVRRLEEKSRTQKARVTELQSQIEALASESVEL
                     SDQIAGLQQEITRGENRSESILQRFRARLRHLHKMRQGTLLTSIFSASDLNTFLNRYQ
                     MIRYLLQHDRELLAELQKVRAKLLEDIDLKKRKQDRLSEVVRLTEKNRDALQVESTSL
                     SAMLQSLLLERQVFLARQKKLKQTYAQLEGELGRIEKTREADPDKFDEEVTRKPGGGR
                     PTPEPPGGAPEPPVRPVAVPPATGSDAPPDLQAATAGATRFQWPFAVAASALPADAAA
                     INWLDLSIGADTEIKAAGAGKVYFKGPLGQFGNTIILAHRQGFTTLYGNLDELWVGLH
                     QVVDPGEVIGMIRAGSGKPLRFEIRFGGKAQNPMKYLPPLR"
     gene            complement(1395..2297)
                     /locus_tag="PKO06_09945"
     CDS             complement(1395..2297)
                     /locus_tag="PKO06_09945"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HNV70010.1"
                     /translation="MNTSSLFFFINEALTNFRRAGLMTVVSVSTIAVSLLMMGTLLLA
                     TLNVEAFLTRLQAEAMVNAFLAPGTSVEIANNLKLQITGMEEVSQVEVITPEQAASDL
                     FLNPEDRHLLDIGLASGPNPLPYTLRIQLRSSADLDPVLKKVKSFSWVESVGYGEEAF
                     RQFRGLSELLWVGSLIVILIMGLCSLFIVANTIRLTLFLRQEEIIIMRLVGATNWFIR
                     WPFVIEGFLQGVIGAVVSVLLLLISYKFILFRLAALIPFFQFDVQTGHLIKLGIKLLL
                     MGMVLGISGALITLRDLRSFGRGT"
     gene            complement(2307..2990)
                     /gene="ftsE"
                     /locus_tag="PKO06_09950"
     CDS             complement(2307..2990)
                     /gene="ftsE"
                     /locus_tag="PKO06_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HNV70011.1"
                     /translation="MIELQNVSKIYPNGVRALNGINLVINPGDFVFLVGPSGAGKSTF
                     LRILFREETPTEGKVVIDGKDIVSMPDSRVPYLRRNMGIVLQDFQLLKLRTVAENVAF
                     GLRVIGAAENVINQRVQEVLEQVGLSHKRRMYPDELSGGEQQRVCIARAIVNSPLILI
                     TDEPTGNLDPMISMEIMQLFMDINTRGTTVIMATHNHHLVNKLRKRVIALVEGRIVKD
                     DPVGTYAYE"
     gene            3218..>3419
                     /locus_tag="PKO06_09955"
     CDS             3218..>3419
                     /locus_tag="PKO06_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HNV70012.1"
                     /translation="MIFELLKMCYDKGASDLHFKVGYPPILRIDGAMVPLKMKVIEQS
                     DYQIMLESILDDYQLVKFLEHKK"
BASE COUNT          739 a          995 c          983 g          702 t
ORIGIN      
        1 cccctcagcg ccgaatccga ttaccgtcaa ccctgctgag tcaaccgcgg caagactgcc
       61 aatggcgaaa cacgtcagga atcagcgcaa cgggggcagg tatttcatcg gattctgggc
      121 tttgccgccg aagcggattt caaagcgcag cggcttgccg gaaccggctc tgatcatgcc
      181 gatgacttcg cctggatcca cgacctgatg caggccgacc cacagttcat ccagattgcc
      241 atacagggtg gtgaagccct gacgatgagc gagaatgatg gtattgccaa actggcccag
      301 cggccccttg aaatagactt tgccggctcc ggcggctttg atctcggtat ccgccccaat
      361 cgagagatcc agccagttga tcgctgccgc gtcggccggc agcgctgatg cggcaaccgc
      421 gaagggccac tgaaagcggg tggcacctgc agtcgcggcc tgcagatcag ggggggcatc
      481 ggacccggtc gccggcggaa ccgccaccgg tcgcacgggt ggctcgggcg ccccccccgg
      541 gggttccggt gtcggtcggc cgccaccagg cttgcgcgtg acttcttcgt caaatttgtc
      601 cgggtcagcc tcacgggtct tctcgatgcg ccccagttca ccctcgagct gtgcataggt
      661 ctgcttgagc ttcttctgac gcgccagaaa cacctgccgt tcgagcagca gactttgcag
      721 catcgccgac agcgaggtgc tttctacctg cagcgcatca cggttttttt cggtcagtcg
      781 caccacttcg gataatcggt cttgctttcg cttcttcaga tcgatatctt ccaacaactt
      841 ggctcggact ttctgcagtt cggccaacag ttcgcgatcg tgctgtaaca ggtagcgaat
      901 catctgatag cgattgagaa atgtgttcag gtcgctcgcg gaaaaaatgc tggtcagcag
      961 ggttccctgt cgcatcttgt gcagatgccg cagtcgcgcg cgaaaccgct gcagaatgct
     1021 ttccgagcga ttctcaccgc gtgtgatttc ctgctgcaac ccggcgatct ggtcagataa
     1081 ctcgaccgat tcgcttgcca gcgcttcgat ctgactctgc agctcggtca cccgggcttt
     1141 ctgggttcgc gatttctctt cgagccggcg cacgtgatcg cgcgaatcgc gcaccttgag
     1201 atcgagaaag tcgatctgca ccttgaccga gtcgagttct tcctgaagct ggtcgatggt
     1261 tcgacgcgcc tggcgcagct tgtccggttc ctgggcgcgc accggcaaaa ccccgaccga
     1321 gaacaggcac gcgccgatca tgcccccgat caacccgtga cgcaggtatc gtgtaacgtt
     1381 catggcgcgc cctttcaggt gccgcggccg aacgaacgca ggtcgcgcag cgtgatcaac
     1441 gctcccgaaa tgcccagcac cattcccatc aggagcagtt tgataccgag tttgatcaga
     1501 tgacccgtct gcacatcgaa ctgaaaaaac gggatcagcg cagcgagtcg gaacagaatg
     1561 aacttgtacg aaatcaacag caacagaacc gacacgacgg cgccgatgac accctgcaag
     1621 aacccttcga tgacgaacgg ccagcgaatg aaccagttgg tcgcgccgac caggcgcatg
     1681 atgatgattt cttcctggcg cagaaacagg gtgaggcgaa tggtgttcgc gacgatgaag
     1741 agcgagcaca gacccatgat aagaataacg atgaggcttc cgacccacag caactcggac
     1801 aaaccgcgaa actgacgaaa cgcctcttcg ccgtatccga cgctctcgac ccacgagaac
     1861 gacttcactt ttttcagcac cgggtcaaga tcggcactcg aacgcaactg aatgcgcagc
     1921 gtatacggca gggggttcgg cccgctggcc aagccaatgt cgagcagatg ccgatcttcg
     1981 gggttgagaa agagatcgct cgcggcctgc tcgggcgtga tcacctcgac ctggctcact
     2041 tcctccatgc cggtgatctg cagcttgaga ttgttggcga tctcaaccga cgtgccgggc
     2101 gcgagaaagg cgttcaccat cgcctcggcc tgcaggcgcg tcagaaacgc ctcgacattc
     2161 agcgttgcca gcagcaacgt gcccatcatc agcagcgata cggcgatcgt cgaaacgctg
     2221 acgacggtca tcaggccggc ccgtcgaaag ttcgtcagcg cttcattgat aaagaagaac
     2281 aaacttgagg tattcatggt ttctgactac tcatacgcat aggtgccgac ggggtcgtcc
     2341 ttgacgatgc gcccttcgac gagggcgatg acgcgcttgc gcagcttgtt gaccaggtgg
     2401 tggttgtgcg tggccatgat cacggttgtg ccgcgggtgt tgatgtccat gaacagttgc
     2461 atgatctcca tcgaaatcat cgggtcgagg ttgccggtcg gctcgtcggt gatcagaatc
     2521 aacggactgt tgacgatcgc acgggcaata cagacgcgct gctgttcgcc gcccgagagt
     2581 tcatcgggat acatgcgccg cttgtgtgac agcccgacct gctccaggac ttcctggaca
     2641 cgctgattga tcacattctc agctgccccg atgacccgca ggccgaaggc gacattctcg
     2701 gcgactgtgc gcagtttcag cagctggaaa tcctgcagga cgatgcccat gttgcgccgc
     2761 agatacggca cacgtgaatc aggcatcgaa acgatatcct tgccgtcgat gacgaccttc
     2821 ccttcggtcg gggtttcttc acggaacaga atgcgcagaa acgtactttt tccggcgccg
     2881 ctggggccga cgagaaaaac gaaatcgccc gggttgataa ccaggttgat gccgttgagg
     2941 gcacgcaccc cgttggggta gattttcgaa acgttctgga gttcaatcat tcgctgggtt
     3001 tgccgggtca gccgaaatgc ctgtttttcc ggcgtgcgta gtatagcgaa taagaaaata
     3061 aaaggcaaaa gccaaaatgc cgataatgga gctcgtcttt tttcttatgc tcagagaaaa
     3121 aggatgtatt atactggtgc gcgattgcgg aaggtgccac cgggcaaccc tcggccggtc
     3181 gagtgagacc gcgccggaac tgtgatggag gaatgggatg atcttcgaac tcctcaagat
     3241 gtgttacgac aagggagcat cggacctgca tttcaaggtc ggatacccgc cgattctccg
     3301 aatcgacggg gccatggtgc ctctcaagat gaaagtcatc gagcaaagcg attaccagat
     3361 catgctcgag tcgattctcg acgactacca gctggtcaag tttctcgaac acaagaagc
//
LOCUS       DAORHD010000431         3402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31251_length_3402_cov_3.638482, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000431 DAORHD010000000
VERSION     DAORHD010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31251_length_3402_cov_3.638482"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2208
                     /locus_tag="PKO06_09960"
     CDS             <1..2208
                     /locus_tag="PKO06_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HNV70013.1"
                     /translation="DRTYRPNLTTKLFDCKGRPLAELHAEENRSRILPASEIPANMKH
                     AIVAIEDERFYQHYGVDLVGISRAMVKNVQAGKVVQGASTLTQQLVKNAFLSSEKTLK
                     RKVTEAMLAFQLERKYSKDEILNLYLNEIYFGHGAYGLDSAAKVYFGKEAKDLTLLEC
                     ATLAGIPKSPVAYSPHKYPENNRMRRELVLSKMVELGFISPSEYETVRKQNPTLIPLV
                     QERFQAPYFVTHVRDILLEKYGANLVYNGGLKIYTTIDLDYQKYAEDAMANAEIMKNR
                     PIEKDPNMNGSLVCIDHRNGHIKAMWGGRDFEHSQFNRVTQAYRQPGSSFKPFVYGCA
                     IENGLLPGDTVVDEPISFTNPWSGKVWAPKNYDLKFHGTVTFIKAIANSFNVPAVKLI
                     DRYSAARVIRFAHKCGLTSPMQPNLSLALGSGNFTPLEMCSAFGVFANQGIRVTPMAI
                     TRIEDRDGNVLEETVPQAQEVMKAAHAAMLTDMLRTAVERGTGKRAMVKGHVVAGKTG
                     TTNNYIDAWFTGFTPELVCTVQFGYDQPKPLGGKSAGGTVSAPVWNAFMSKVLNDYPS
                     SEFPVPEGCARIKVCMLSGKPASKSCPGAVVVPQVYPLEAIPKIECPYHAGGGRFEVT
                     EDGEVEFAQGPLAMVAPVDNTEDFFRDDFRKAEATSGAKNPTAETGGDAATDEEEYEG
                     SGIGPDDAATDEEDEAAAYGAGSAGPPKVQVDDEPREPGVVMKPNVEFKEDFE"
     gene            complement(2251..>3402)
                     /locus_tag="PKO06_09965"
     CDS             complement(2251..>3402)
                     /locus_tag="PKO06_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV70014.1"
                     /translation="ELPVIICTAYGSIPSAVEAVREGAVDYLAKPLPHVDDLTRSVQR
                     AIEQRDLRRQNRTLLQQTRDPDPFPAQDPLMVGILEKARRAAASDVSILITGESGTGK
                     ERLARFIHQQSRRSERPLVAVNCAAIVDTLLESELFGHEKGAFTGATERRSGRFEEAH
                     LGTLFLDEIGELDLALQPKLLRALQEREIRRVGGDRLIRVDTRLITATNRDLREQVKS
                     GTFREDLYYRLSVLVLRIPPLRERREDILYLADRFLRERRQQFARPELKLSPRALDRL
                     SRHAWPGNVRELSNVIEASTLLCEGNLIDEQDLFGLEGDGAELTSSVPDVTSLSPIEA
                     AERQTIVRALEASRGNRAQTAQLLGMSTRNLLYKLKKYDLGGGNRRSRS"
BASE COUNT          774 a         1013 c          926 g          689 t
ORIGIN      
        1 gatcgcactt accgcccgaa tttgacgacc aaactcttcg actgcaaggg ccgaccgctc
       61 gccgaactgc atgccgaaga gaatcggtca cgtattttgc cggcttctga gattccggcc
      121 aatatgaaac atgcgattgt ggccatcgaa gatgagcggt tctatcaaca ttacggcgtt
      181 gacctggtgg gcatttcacg ggccatggtc aagaacgtcc aggccggcaa ggtcgtacaa
      241 ggcgccagca cgctgactca gcagctggtc aagaatgcgt ttctgtcgag tgagaagacg
      301 ctgaaacgca aggtcaccga agccatgctc gcgtttcagc tcgagcgcaa gtattccaag
      361 gatgagattc tcaatctgta cctgaatgag atttatttcg gtcacggagc ctacgggctt
      421 gattccgccg ccaaagtcta ctttggcaag gaggccaagg atctcactct gcttgagtgc
      481 gccacgctcg ccggcattcc gaaaagtccg gtcgcctatt ccccacacaa gtacccggaa
      541 aacaaccgca tgcggcggga attggttttg tccaagatgg tcgagcttgg tttcatctct
      601 ccaagcgagt atgagacggt acgaaagcag aacccgacac tgattcccct ggtgcaggaa
      661 cgctttcagg ccccgtattt cgtcacgcat gtacgtgaca ttctgctcga gaaatacggc
      721 gcaaatctcg tctacaacgg cggtttaaag atctacacca ccattgacct cgactaccag
      781 aagtatgccg aagatgccat ggccaatgcc gagatcatga aaaatcgacc gatcgaaaaa
      841 gaccccaaca tgaacggttc tctggtatgc atcgatcatc gtaacgggca catcaaggcg
      901 atgtggggcg ggcgcgactt cgagcattcc cagttcaacc gtgtcacgca ggcctatcga
      961 cagccgggtt cgtccttcaa acccttcgtc tatggctgcg ccatcgagaa cggtctgctg
     1021 cccggcgata cagtcgttga tgagccgatc agtttcacca acccctggtc gggcaaggtg
     1081 tgggcaccga agaactacga tctcaagttt cacggtacgg tcaccttcat caaagccatt
     1141 gccaacagct tcaacgtccc ggcggttaaa ctgattgatc ggtactccgc ggcccgggtc
     1201 attcgatttg cgcacaaatg cggtctgaca tcgccgatgc aaccgaacct gtcactcgcc
     1261 ctcggatcgg gaaactttac gccgctcgaa atgtgctcgg ccttcggtgt cttcgccaat
     1321 cagggcattc gcgtaacccc gatggccatc acgcgtatcg aagatcgtga cggaaacgtt
     1381 ctcgaagaaa cggttccgca ggcacaagag gtcatgaaag ccgcccatgc cgcgatgctg
     1441 accgacatgc tgcgcacggc cgtcgaacgc ggcactggga aacgagccat ggtcaaaggt
     1501 cacgtcgtgg ccggcaaaac cggaaccacg aacaattaca tcgacgcctg gtttaccgga
     1561 ttcactcctg aactggtctg cacggttcag ttcggctatg accagcccaa gcctctcggc
     1621 ggcaaaagcg ccggtggcac ggtttcggca cccgtctgga acgcattcat gtcgaaagtt
     1681 ctcaacgatt accccagcag tgaatttccc gttcccgaag gttgcgcccg catcaaggtc
     1741 tgcatgctga gtggcaagcc agccagcaag tcttgccccg gcgcggtcgt cgtgccgcag
     1801 gtctatccgc ttgaagccat tcccaaaatc gagtgtccct accacgccgg cgggggtcgc
     1861 ttcgaggtga ccgaagatgg tgaagtcgaa ttcgcccaag gtccgctcgc catggtggcc
     1921 ccggtcgata ataccgaaga cttcttccgt gacgatttcc gcaaggcgga agcgaccagc
     1981 ggcgccaaga atccgaccgc agaaaccggc ggtgatgccg cgaccgacga agaagagtat
     2041 gaaggcagcg gtatcgggcc ggatgacgcc gccaccgatg aagaagacga ggcagccgct
     2101 tacggcgccg gctcggcagg cccaccaaaa gtccaggttg acgatgaacc tcgcgagcct
     2161 ggcgtggtga tgaagcccaa cgtcgaattc aaagaagatt tcgagtaacg cacaagcagc
     2221 aacgtcttct gctgtctgtt ttcaagaaat ctacgatctg ctgcggcggt tgcctccacc
     2281 aagatcgtac tttttgagct tatagagaag atttcttgtc gacattccca gcaactgagc
     2341 agtctgagcc cgattccccc tgctggcctc gagggctcga acgatcgtct gccgctcggc
     2401 tgcctcgatc ggagataacg aggtcacgtc gggcacggaa ctcgtcagtt ccgcaccatc
     2461 cccttccagt ccgaagaggt cctgctcatc gatcagattg ccttcacaga gaagtgtgga
     2521 tgcctcgatg acattcgaca gctcacggac atttccgggc caggcgtgac gcgatagcct
     2581 gtcgagagcg cgtggcgaca gcttcaattc agggcgggca aactgttgcc gcctctcgcg
     2641 taaaaatctg tcagccagat agagaatatc ctctcgtcgc tcccggagag gcggtatgcg
     2701 cagaacaagc accgacaatc gatagtacag atcctctcga aatgtcccgg acttcacctg
     2761 ttcgcgaaga tcacggttcg tcgcggtgat caggcgagta tcgacgcgaa tcaaccggtc
     2821 tcccccgacg cgccgaattt ctcgctcctg caacgcacgc agcaactttg gctgcagagc
     2881 aagatccaac tcgccaatct catccaggaa cagcgtgccg aggtgtgcct cctcgaatcg
     2941 tccacttcga cgctcggtgg ctccggtgaa cgctcccttt tcatgtccga aaagttcgct
     3001 ttccaaaaga gtgtcgacga ttgcagcgca gttcacggcc accagcgggc gttcgcttcg
     3061 tcggctttgt tgatgaatga accgcgccaa tcgctccttc ccagttcctg attccccggt
     3121 gatcaggatc gacacatcgg aagccgcggc acggcgtgct ttttcaagga ttcccaccat
     3181 cagcggatct tgggcgggga agggatccgg atcacgggtt tgctgcaaca gcgttcgatt
     3241 ctgacgacgc agatcacgct gctcgatcgc tcgctgcaca ctgcgtgtca aatcgtcaac
     3301 atgcggaaga ggcttggcaa gatagtctac ggccccctcg cgtaccgcct cgactgccga
     3361 cggaatcgag ccataggccg tgcaaatgat gacgggcagc tc
//
LOCUS       DAORHD010000432         3398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31298_length_3398_cov_3.200718, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000432 DAORHD010000000
VERSION     DAORHD010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31298_length_3398_cov_3.200718"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3398
                     /locus_tag="PKO06_09970"
     CDS             <1..>3398
                     /locus_tag="PKO06_09970"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70015.1"
                     /translation="VIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLIK
                     LMAPDIIIKNEKRMLQEAVNALIDNGRRGKLVTGPNNRPLKSLTDLLKGKQGRFRQNL
                     LGKRVDYSGRSVIVVGPKLKLHQAGLPKKMALELFKPFVMNRLVQKGTAHNIKSAKKM
                     IERERSEVWDVLEDVIEHHPILLNRAPTLHRLGIQAFEPVIIEGKAIQIHPLVCAAYN
                     ADFDGDQMAVHVPLLLDAQIEARMLMMSSNNILKPADGLPVTHPTQDMVIGLFYLTVE
                     KPEGSDYPSMVLEKGNTKSWSNLVLDKNRIFDFKLAAEIVNDKDEVVLETGTVIDSKE
                     VLERLDAAKVDEIEVYNYPVFHSIDEAVNAYQAAGFDLQYPIYIHRKAFREEKVVNLP
                     YDPDIRSSSYVRTTIGRLVFNNAFPVEITYRNQAIRKGDVNTLIKQLYTVCKLTIVGE
                     TLDILKELGFRYATLSGLTISVKDLVTPPKKRELLDQADRKAAKVREQWAHGLLSRDE
                     RKQSEVNIWTKATEDVSDDLLQEFESSAKKGEFNPVYMMAFSGARGNMQQIRQLAAMR
                     GLMSNPRGDILAFPIKSNFREGLNQTEYFISTYGARKGLVDTALRTADSGYLTRRLVD
                     VAQDVVITIGDCHTRNGIEVLPIRQARSSNNITVDDIVVPIRYKIAGRVLARPVVHPV
                     SGDPISVRVKGKDIKLDANLYVTDEIANELEAAIEFNVKTEDLREDMIVAEQILDPKT
                     NRLILRPDRRLNSYVINRITELGFPELKVRSALTVRSPLSCMAKRGICQMCYGADLAT
                     NRKVDIGEAVGIIAAQSIGEPGTQLTMRTFHLGGVALAQRSFVKARTAGVVRLSQLKL
                     AKISDRAKFEIGSGTETFDKSEFGASIRTVVVGGHVIIEGKGARKERVHIPLGSELKV
                     EDGDKASPGKPLAEYNPNTVVTGYTGEVIFDNIEVKDGMVVSENGSIQILTQEFDPDT
                     KDFHVEEYKIPQGSTLKVEDNEIIHAGNPLAEVSAEQHAAIARTDGICKFENVRFKNR
                     QVISDNGLIFIFPEGSTKKDMRDYPLPKGVKETREIATAKTDGMLLNVKNGDMVVAGE
                     RLLSVVSEIDGEVTISANGTQITVARDVVEEYEFKGELQAQINK"
BASE COUNT          783 a          998 c          943 g          674 t
ORIGIN      
        1 tgtcattccg gtcattcccc ccgatctgcg ccccatggtc cagctcgatg gcggccgctt
       61 tgccaccagc gatctgaacg acctgtatcg tcgcgtgatc aaccgcaaca accgtctcaa
      121 gcgtctgatc aagctcatgg cgcccgatat catcatcaag aacgaaaaac gcatgctgca
      181 ggaagccgtc aatgctctga tcgacaacgg ccgccgcggc aagctggtca ccggtcccaa
      241 caatcgcccg ctcaagtcgc tcaccgacct gttgaagggc aagcagggcc gtttccgcca
      301 gaacctgctc ggaaagcgcg tcgactactc gggccgctcg gttatcgtcg tcggtccgaa
      361 gttgaagctg catcaggcgg gtctgccgaa gaagatggct ctcgagctgt tcaagccgtt
      421 tgtcatgaac cgcctggttc agaagggcac ggctcacaac atcaagagtg ccaaaaagat
      481 gattgaacgc gaacgctcag aagtctggga tgtcctcgaa gacgttatcg aacatcaccc
      541 gattctgctc aaccgcgctc cgacgctgca ccgcctcggc attcaggcgt tcgagcccgt
      601 catcatcgag ggcaaggcca ttcagattca ccctctcgtc tgcgccgcct acaacgctga
      661 cttcgacggt gaccagatgg ccgttcacgt gccgctgctg ctcgacgctc agatcgaagc
      721 ccgcatgctg atgatgtcgt cgaacaacat tctcaagcct gctgacggtc tgccggtcac
      781 gcatccgaca caggatatgg tcatcggtct gttctacctg acggtcgaga agcccgaagg
      841 gtctgattat ccgtcgatgg tcctcgagaa gggcaatacc aagagctggt cgaacctggt
      901 gctcgacaag aatcgcatct tcgacttcaa actcgcggct gaaattgtca acgacaagga
      961 tgaagtcgtt ctcgaaaccg gtaccgtgat cgattccaaa gaagtcctgg aacgactgga
     1021 cgccgccaag gttgacgaga tcgaagtgta caactacccg gtcttccaca gcatcgatga
     1081 agcggtcaac gcctatcagg cagccggttt cgacctgcag tatccgatct acatccatcg
     1141 caaggcattc cgcgaggaaa aggtcgtcaa tctgccctac gatcccgaca tccgcagttc
     1201 gagctatgtg cgcaccacca tcgggcgcct ggtcttcaac aacgcgttcc cggtcgagat
     1261 cacctatcgg aaccaggcga ttcgcaaggg cgatgtgaac actctgatca agcagctcta
     1321 caccgtctgc aagctcacca tcgtcggcga gacgctcgac attctcaaag agcttggatt
     1381 ccgctatgcc acgctttccg gcctcaccat ctcggtcaaa gatctggtca ctccgcccaa
     1441 gaagcgcgag ttgctcgatc aggccgatcg taaagccgct aaagtgcgcg aacagtgggc
     1501 gcacggcctg ctgagccgcg acgaacgcaa acagtcagag gtcaacatct ggaccaaagc
     1561 cacggaagac gtttccgacg atctgttgca ggaattcgag tcatcagcca agaagggtga
     1621 gttcaacccg gtttacatga tggcgttctc cggagcccgc ggaaacatgc agcagatccg
     1681 ccagctggcc gctatgcgcg gtctgatgtc gaatccgcgt ggcgacatct tggccttccc
     1741 gatcaagtcc aacttccgcg aaggtctgaa ccagaccgaa tacttcattt cgacctacgg
     1801 cgcccgcaag ggtctggtcg ataccgctct ccgcaccgct gactccggat acctgacccg
     1861 ccgtctcgtc gatgtcgcgc aggatgtggt tatcaccatc ggtgactgcc acacccgcaa
     1921 cggcatcgaa gtgctgccga ttcgtcaggc tcgctcgtcg aacaatatca ccgtcgacga
     1981 tatcgttgtg ccgattcgct acaagattgc tggtcgcgtt ctggcccggc cggtggttca
     2041 cccggtgtcg ggagacccga tctccgtgag ggtgaagggc aaggacatca agctcgatgc
     2101 gaatctctac gtcactgacg agatcgcgaa tgagctcgaa gcggcgatcg aattcaacgt
     2161 caagaccgaa gatctgcgcg aagacatgat cgtcgccgaa cagattctcg atccgaagac
     2221 caaccgtctg attctgcggc cagaccgccg cttgaacagc tacgtcatca accgtatcac
     2281 cgaactgggt ttcccggaac tcaaagtgcg cagtgcgttg accgtgcgct cgccgttgag
     2341 ctgcatggca aaacgcggca tctgccagat gtgctacggt gccgacttgg cgacgaaccg
     2401 caaagtcgat atcggcgaag ccgttggaat tatcgcggcc cagtcgatcg gtgaacctgg
     2461 aacgcagctg acgatgcgca cattccactt gggcggcgtc gctctggcgc agcgctcgtt
     2521 tgtgaaggcc cgtacggccg gtgtggtccg cttgagtcaa ctcaaactgg cgaagatttc
     2581 tgaccgcgcg aagttcgaga ttggttccgg tactgaaacg ttcgataaga gcgaattcgg
     2641 tgccagcatt cgcaccgtcg tcgtcggtgg tcatgtcatc atcgaaggga agggtgcccg
     2701 caaagaacgc gttcacattc cgctcggttc agaactcaag gtcgaagacg gcgacaaggc
     2761 ctcgccgggc aaaccgctgg ccgagtacaa cccgaatacg gttgtcacgg gctacacggg
     2821 cgaagtcatc tttgacaaca tcgaagtcaa agatggtatg gtcgtctccg agaacggttc
     2881 gattcagatt ctgactcagg aattcgatcc ggacaccaaa gacttccatg tcgaagaata
     2941 caagattcct cagggatcga cgctcaaggt tgaggacaac gaaatcattc acgccggaaa
     3001 cccgctggct gaagtttccg ccgagcagca cgccgccatt gcgcgcactg atggtatctg
     3061 caagttcgag aatgtccggt tcaagaaccg ccaggtcatc agcgacaacg gcttgatctt
     3121 catcttcccc gaggggtcga cgaagaagga tatgcgcgat tatccgctgc cgaagggtgt
     3181 gaaggaaacc cgtgagattg cgaccgcaaa gaccgatggc atgctgctga atgtgaagaa
     3241 cggcgacatg gtcgtggccg gtgagcgatt gctctccgtg gtttctgaga tcgatggcga
     3301 agtgacgatc tcggcaaatg gtactcagat taccgtagct cgtgatgtcg tcgaagagta
     3361 cgagttcaag ggtgagctgc aggcgcagat caacaagg
//
LOCUS       DAORHD010000433         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31308_length_3397_cov_3.184321, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000433 DAORHD010000000
VERSION     DAORHD010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31308_length_3397_cov_3.184321"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="PKO06_09975"
     CDS             complement(<1..837)
                     /locus_tag="PKO06_09975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV70016.1"
                     /translation="MRCRQSRLEQALELHPTGIVTVAGVDLRIVSLNQAARQLADDIE
                     GFDIGFSLIELERRGYTWQRETGETMTACDLAFARAMRERRPVRDEFVKLSYPNGRFR
                     WFLTNASPLPDAEGEEAAALGFLADSTERKMHDEERRRLDVRLTQAQKLESMSVMAGG
                     IAHDFNNLLMTMMGHADIALSEMSPMAPSRENVVQILNAARRAADLTRQMLIYTGKGR
                     TTIELLNLQEMIEEMTHLLEVSVSKKSHLRFQFAPDLPLIDGEPTQIRQVLMNLVINA
                     SEA"
     gene            complement(881..3055)
                     /locus_tag="PKO06_09980"
     CDS             complement(881..3055)
                     /locus_tag="PKO06_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV70017.1"
                     /translation="MTQQRSREKDVASLEREIESLRTRVKELEQEHHEVSRQTWSSLS
                     LHAIESLHDMVFWTRPDMTIEYVNESACRVLEYSRVELLRMTVSDLDPNIHPERWGPY
                     WERLRQDGFIRLETTQRTKSGRLIPVEVLATFLTIEGQELSCTVIREIADRRKAEDDL
                     RASEARFRTLFLNMAEGVALHRIVHDDRGAMVNYEILDVNPRYEEILGIKRADVIGRT
                     GTEVYGTTQPPYLSEFGSVAMTGVPYVFETYFAPFDKHFQISVVSPGKGFFCTIFFDV
                     SSHKRLEQELRDRLLALTQPVGTVGTLRFEDLFGVEDLQSLQDSLALSCGVTAVLTQP
                     DGTLITRVSLPSGENALPVERREVRELRPGDVWKTDPALPVPNLKEPVLRPEGGTEPK
                     EAAVGFWVEERLLATWFLFPGSRRGLRNRQAVERLNSAARAFFLVAQRLSQLALQNRQ
                     QVGAILQLEKTEERIRRLNVEWMGKNAELENVVHAAANDLHGPLMNVRGFTEQIRKTA
                     GEAVAESQTVPVDKQRMRHLNELLEERIPRSIAFIHTDVNKMETLIGGLLRFSQLGWV
                     RIHLERLDARKLVEQVLQSMSATIQGVRASVQIENLPDCHADAEQMREVFSIFFENAV
                     KYRHGYRPLKIRVFGRVEGQMTTFGVEDNGLGIKPEQQPRVWDFFQRLRQRDESRGDG
                     MGLTLVRRIIDRHHGSVFVESDGANGSTFYFKIPLVQETVHD"
     gene            complement(3091..>3397)
                     /locus_tag="PKO06_09985"
     CDS             complement(3091..>3397)
                     /locus_tag="PKO06_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70018.1"
                     /translation="KMQLRPPQAPKEFIGVQSQISVNRGGPYQYFVIITKPELKLKAA
                     AGSDQDVVNLERKPDVHILVVRQVATKTGGYQTNASDVSRLVAIVKQSVEKTLEIQK"
BASE COUNT          701 a         1061 c          886 g          749 t
ORIGIN      
        1 cgcctcggac gcattgatca ccagattcat aagcacctgt ctgatctgcg tcggttcgcc
       61 atcgatcagc ggcagatcag gagcgaactg aaagcgcaga tgcgactttt tcgagaccga
      121 cacctcgagc aggtgggtca tctcctcaat catttcctgc agattaagca gttcgatcgt
      181 cgtacgccct ttgccggtgt agatcagcat ctgccgggtc aggtcggccg cccggcgcgc
      241 cgcattgaga atctgcacga cgttctcacg cgacggcgcc atcggagaca tttcggacaa
      301 tgcgatgtcg gcgtgcccca tcatggtcat cagcagattg ttaaagtcgt gcgcgatgcc
      361 gccggccatg accgacatgc tttccagttt ctgtgcctgc gtcagtctca catccagacg
      421 tcggcgttcc tcgtcgtgca tcttgcgttc ggtactgtct gccagaaaac cgagtgccgc
      481 cgcttcttcc ccttcggcat cggggagcgg agaagcattg gtcagaaacc agcggaagcg
      541 tccgttgggg taggacaact tgacaaactc atcacgcacc gggcgccgtt cgcgcatcgc
      601 acgggcaaaa gccagatcac acgcggtcat cgtttcgccc gtctcgcgct gccacgtata
      661 gccacggcgc tcaagctcga taagcgaaaa gccgatatcg aatccttcga tgtcatcggc
      721 taattggcgt gcggcctgat tcagcgagac aatgcgcaga tcaacaccag ccacggtcac
      781 gattcccgtc ggatgcagtt caagagcctg ttcgagccgg ctctgacgac accgcatctc
      841 gccatcactc ctgtttgtcc ggggttgatt cgggtttccc tcagtcatgc acggtctcct
      901 gcaccagtgg aatcttgaaa tagaaagttg aaccgttcgc tccgtctgat tcgacgaaca
      961 cactcccatg gtgccgatcg atgatgcgac gcaccagcgt caggcccatg ccgtcgcccc
     1021 tgctttcatc acgttgtcgc aggcgttgaa agaaatccca gacacgaggc tgctgttcag
     1081 gcttgatgcc gaggccgtta tcctcgaccc cgaaggtagt catctgtccc tcgacacggc
     1141 cgaacacccg aatcttcagc ggacgatacc catgtcgata cttgaccgcg ttctcgaaaa
     1201 agatggaaaa aacctctcgc atctgttcgg cgtcggcatg gcagtctggc aggttctcga
     1261 tctgaacgct ggcacgaacc ccctgaatcg ttgctgacat actctgcagc acctgctcga
     1321 ccagtttgcg cgcgtccaat cgttcgaggt gaattctgac ccaccccaac tgggaaaacc
     1381 tcagcaatcc gccgatcaag gtttccattt tattcacatc ggtgtggatg aacgcgatcg
     1441 aacgcggaat acgctcttcg agaagttcgt tgagatgccg catacgctgt ttatccaccg
     1501 gtactgtctg cgactcggcc accgcctcgc ctgcggtctt gcgtatctgc tcggtgaacc
     1561 cgcgaacatt catcaacggg ccgtgcagat cattcgcagc ggcatgaacc acgttttcga
     1621 gctcggcatt cttccccatc cactccacat tcaacctgcg tatgcgttct tccgttttct
     1681 cgagctggag aatcgccccg acctgttgtc gattctgcag tgccaactgc gacaaccgtt
     1741 gcgcaaccag aaaaaaggcg cgggccgctg aattcaggcg ctcgaccgct tgacgatttc
     1801 ggagaccgcg cctcgaacca ggaaaaagaa accacgtcgc cagaaggcgc tcttcgaccc
     1861 agaagccgac agcggcctcc ttcggctcag tgccgccctc cgggcgcagg acaggctctt
     1921 tcagattcgg aaccggcaat gccgggtcgg ttttccagac atcgccggga cgcagttcgc
     1981 gaacctctcg cctctcgacc ggcaaagcgt tctctcccga cggcaggctc actcgcgtga
     2041 tcagagtacc atccggctgt gtcagaacag cagtcacacc gcagctgagc gccagtgaat
     2101 cctgcagcga ctgcaggtct tcaacgccaa agagatcttc gaaacgaagg gttccgactg
     2161 tgcccacggg ctgcgtcagc gccagtaatc gatcgcgcag ttcctgttcg agtcgtttgt
     2221 ggcttgaaac atcgaagaag atcgtgcaga agaaaccctt acccggcgag accacggaga
     2281 tctgaaaatg tttgtcgaac ggagcgaagt acgtttcaaa cacatagggt acacccgtca
     2341 tcgccacgct accgaactca gaaagatacg gcggctgcgt cgtaccgtag acttccgtcc
     2401 cagttcttcc gattacatcg gcgcgtttga tgccgagaat ctcttcatac cttgggttca
     2461 catcgagaat ctcgtagttg accatcgccc cccggtcgtc gtggacgatc cgatgcaggg
     2521 ccaccccttc ggccatgttg agaaacaacg tgcgaaaccg cgcttcactg gctcgcagat
     2581 cgtcttcggc tttccggcga tccgcgatct cgcgaatgac ggtgcaactg agctcctggc
     2641 cctcgatcgt cagaaaggtc gccagaactt cgaccgggat caggcgccct gacttcgttc
     2701 gctgggtggt ctcgagacgt atgaacccgt cctgacgcag gcgctcccaa tacgggcccc
     2761 agcgttccgg gtgaatattt ggatccagat cggaaacggt catacgaagc agctcgacac
     2821 gcgagtattc gagcacccgg caggcagact cgttgacata ctcgatggtc atgtccggtc
     2881 tggtccagaa caccatgtca tgaagacttt cgatggcatg cagactgagc gacgaccacg
     2941 tctgccggga aacctcgtgg tgctcctgtt ccagttcctt cacgcgggtt cgtaatgact
     3001 cgatctctcg ttcgagcgac gccacatcct tctctctcga tctctgttgg gtcatggtct
     3061 ccctctcatg ttttcccggt ccgtctcaca tcacttctgt atttccaggg ttttctcaac
     3121 cgactgtttc acgatcgcca ccagtcgcga cacatcactg gcgttggtct ggtacccacc
     3181 tgtcttggtt gccacctgtc tgaccaccag aatgtgaaca tccggtttcc gctccaagtt
     3241 taccacatcc tggtcagaac ctgcagcagc cttcaacttc agttcgggct tcgtgatgat
     3301 cacgaaatac tgatacggcc ccccccgatt cacggagatc tgtgactgca cgccgatgaa
     3361 ctctttcggt gcctggggcg ggcgcagctg catcttg
//
LOCUS       DAORHD010000434         3397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31310_length_3397_cov_2.771095, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000434 DAORHD010000000
VERSION     DAORHD010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31310_length_3397_cov_2.771095"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..342)
                     /locus_tag="PKO06_09990"
     CDS             complement(133..342)
                     /locus_tag="PKO06_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNV70019.1"
                     /translation="MIHLTRLNGTRFVLNAEKIVMVEATPDTIVTLGRETTFRVKETV
                     DEVIRLVIRYRRRIHRLPRTRTRTG"
     gene            complement(361..1908)
                     /locus_tag="PKO06_09995"
     CDS             complement(361..1908)
                     /locus_tag="PKO06_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNV70020.1"
                     /translation="MNQTFESNGLRATAYYDSVTSRFQIVSNEPGDTKELSITNATGP
                     FSDLQLPTGPNRGIGGTKPEVFTDNTVVGSSFEANDFVPAADIQFTVTDRSGHVAAIQ
                     LPAVVAGTQQHYSRSAILSIINSQLAANNVNASATMVDTDDDGTPDQMLLQGRQTGAG
                     EYLTIADVTNINLLGLGTGQFRGTAATSTFTQGGLNFTLTEGTNAWVPNDAMTMTTTG
                     QKGQAESVNIFVPQPNSKELVFSTNVNGEEFKISGAVSKGAVHTTSIAVYDSLGTAHN
                     LVTTWEHTNKSTQEWTYKLSYDKNDPEIEAWMKDPANGVVDPKSPTDSEYERANDALI
                     ANRKGTIYFFNNGKPDLAKSYIPTANMTPKGSNPVSISLDMELVTQFNSAFTTAAREQ
                     DGYEMGLLESVYFEETGIIRGVYSNGQKQPIGLLAVATFNNPGGLEKMGKNMYSISPN
                     SGGAIIGRPATGERGQIVSGSLEMSNVDIAAEFTNMIITQRAFQANSRVITTSDELLQ
                     EVVNLKR"
     gene            complement(1966..2553)
                     /locus_tag="PKO06_10000"
     CDS             complement(1966..2553)
                     /locus_tag="PKO06_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70021.1"
                     /translation="MSNLPGGIWTPTQDESFEISLQPSLGNSITTTVNFTNGIPYSAN
                     DVANIMTNAINAASMPATVQVNGSQISVIPNNSGDVMRTVSPRTATVTFPRFQQGSLQ
                     SSMTLVGGTWTPTNNMTFDVVRRTQAGSSFTSTIAFTAGTAYTPADVQNAIQTALGAN
                     ATVNVVGGNRLEVIPTASSDSLSITNVQPALPNTD"
     gene            complement(2655..>3397)
                     /locus_tag="PKO06_10005"
     CDS             complement(2655..>3397)
                     /locus_tag="PKO06_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70022.1"
                     /translation="YTRDGNLSINPAYDLVATNTGFGIQGWMATQDPKTGNLEVNNAG
                     TVPESINLVRYLKKHAHQTNNITYQSNLDSGSDERDIEFGLDTLTFKDNTGEFQSLKV
                     KFKKIDAQHWVWSATDDTQGNVANGYITTDADGNITASTVEPAGVNMTQGKPFFTYDP
                     DGVPMPASASGLVNATTNAGNGSSSNISASGTEVVDETISVVFDGGDPTRATSYRVIG
                     SQRGFIGSGVLGGEQARLNGNPVSFGGT"
BASE COUNT          613 a          884 c         1096 g          804 t
ORIGIN      
        1 gcttttttgc tggtccagta ggagacaccg ataaccgcca ggacgacgat catcagaagc
       61 agaacgatca gaatgacctt ggtgcgtgga ttcataggcg tatcatacca tccctgtcgt
      121 caaaatgcgc cgctatccgg tgcgtgtccg ggtgcgcggc aggcggtgga tgcggcgtcg
      181 gtagcggatc accagccgaa tcacctcatc caccgtctcc ttcacgcgaa aggtcgtttc
      241 tcggccgagg gtgacgatcg tatcaggagt ggcttcgacc atcacgatct tctcggcgtt
      301 caaaacgaac cgggttccgt tcaatctggt cagatgaatc atgctctact caacgaatcc
      361 ttagcgcttc agattcacga cttcctgcag cagttcgtca gaagtcgtga tgacacgaga
      421 gttcgcctgg aacgcgcgct gagtgatgat catgttggtg aactcggcgg cgatgtccac
      481 gttgctcatt tccagtgacc ctgaaacgat ctggccacgc tcaccggttg ccggtcggcc
      541 aatgatggct ccgcccgagt tcggcgagat ggaatacatg ttcttgccca tcttctcgag
      601 accgcccggg ttgttgaagg tcgcgacggc cagcagaccg atcggctgct tttgcccgtt
      661 cgaatacacg ccgcgaatga tgccggtttc ttcgaagtag accgattcga gcaatcccat
      721 ctcgtagccg tcctgttcgc gggcggcggt ggtgaaggcg gaattgaact gcgtcaccag
      781 ctccatgtcc aggctgatgc tcaccggatt actgcccttg ggcgtcatgt tggcggttgg
      841 aatatacgat ttcgcgaggt cgggtttgcc gttgttgaag aagtagatcg tcccttttcg
      901 attggcaatc aacgcatcat tggcgcgttc atactcagag tcggtaggac tcttgggatc
      961 gacgacgcca tttgccgggt ctttcatcca ggcttcgatt tccgggtcgt tcttgtcgta
     1021 gctcagtttg taggtccatt cctgagtgct cttgttcgtg tgttcccacg tggtgaccag
     1081 gttgtgcgcg gtgccgagcg aatcataaac cgcgatgctg gtggtatgaa cggctccctt
     1141 cgacactgcg cccgagatct tgaattcctc gccgttcacg ttggtcgaga aaacgagttc
     1201 tttggaattc ggctggggaa cgaagatgtt gaccgattct gcctgccctt tctggccagt
     1261 cgtcgtcatg gtcatcgcat cgtttggcac ccacgcgttt gtcccttcgg tcagggtgaa
     1321 gttgaggccg ccctgggtga aggtgctggt cgccgctgtt ccgcggaact ggccggtccc
     1381 gagaccgagc aggttgatgt tggtgacatc cgcaatggtc agatactcgc cggcgccggt
     1441 ctgtcttccc tgaagcagca tctggtcagg agtaccgtca tcgtcggtgt cgaccatggt
     1501 cgccgacgca ttgacgttgt tggctgccaa ctgggagttg atgatcgaga gaatcgcgct
     1561 gcgcgagtag tgttgctgcg tgccggcaac cacggcagga agctggatgg ctgcgacatg
     1621 gccgcttcgg tcggtgacgg tgaactgaat atccgcggcg ggcacgaagt cattcgcctc
     1681 gaaggaggat ccgacgacgg tgttgtcggt gaagacttcg ggcttggtgc caccgatacc
     1741 gcgattgggg ccggtgggaa gctggagatc cgagaacggg cccgtggcgt tggtgatcga
     1801 cagttccttg gtgtcgcccg gttcattgct gacaatctga aagcgggaag tgacggagtc
     1861 atagtaggcg gtcgcgcgca ggccattcga ctcgaaggtc tggttcatgg tctcgatgat
     1921 ctgtgacgtg gtatagcccg acgaagagcg gaagccgaga ttgtcctagt cggtgttggg
     1981 caaggcgggc tgtacattgg tgattgagag tgagtctgac gacgcggtcg gaatcacttc
     2041 aaggcgattt ccgccgacca cgttcaccgt ggcgttcgcg ccaagagcgg tttgaatggc
     2101 attctgaaca tcggcagggg tatacgcggt accggcggta aacgcgatgg tgctggtgaa
     2161 tgaagatcca gcctgggtgc ggcgaaccac gtcgaaggtc atgttgttcg tgggagtcca
     2221 ggtgccgccg accagggtca tgctgctctg cagcgatccc tgctggaacc gcggaaaggt
     2281 gacggtggcc gtgcgcgggc tgacggttcg catcacatca cctgaattgt tgggtatgac
     2341 agagatctgg ctgccattca cttgtacagt cgccggcatg gatgccgcgt tgatagcgtt
     2401 ggtcatgatg ttggcgacat cgttggcgct gtagggaatc ccgttggtga agttgacggt
     2461 agtcgtaatc gagttgccga gcgagggctg caggctgatt tcgaacgact catcctgggt
     2521 cggggtccag atgccgcccg gcagattcga caagtctgag aacaggccgc cggtcagcgt
     2581 gcgtgagaag ttgagtgggg gcgggtagtt ggcgtctcga tcgttgacag tgaacgtgac
     2641 gtcgtttgcc ggattcaggt gccgccgaac gagaccggat tgccgttgag gcgagcctgt
     2701 tcgccgccga gaacgcccga gccgataaaa ccacgctgcg agccgatgac gcgatacgag
     2761 gtggcgcggg tggggtcacc gccatcgaag acgacactga tggtttcatc gacgacttcc
     2821 gtgccgctgg ccgagatgtt gctcgagctg ccgtttcctg cgttggtcgt ggcattcacc
     2881 agaccggatg cactcgcggg catcggcact ccatcgggat cgtaggtgaa gaagggtttt
     2941 ccctgggtca tgttgacccc agcgggttcg acggtgctgg cggtgatgtt gccgtcggca
     3001 tcggtcgtga tgtagccgtt ggccacgttg ccctgggtgt cgtctgtggc gctccagacc
     3061 cagtgttgcg cgtcaatttt cttgaatttc accttcaaac tctgaaactc accagtattg
     3121 tctttgaagg tgagggtatc gaggccgaac tcgatgtcgc gctcatccga gccgctgtcg
     3181 aggttcgact gataggtgat gttgttggtc tgatgcgcat gtttcttcag gtagcgcacc
     3241 aggttgatac tttcaggcac ggtgccggcg ttgttgacct cgaggttgcc ggtcttcggg
     3301 tcctgggttg ccatccagcc ctgaatgccg aacccggtgt tggttgccac caggtcatat
     3361 gcagggttga tgctcagatt gccgtcgcgg gtatagg
//
LOCUS       DAORHD010000435         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31350_length_3394_cov_3.805331, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000435 DAORHD010000000
VERSION     DAORHD010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31350_length_3394_cov_3.805331"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKO06_10010"
     CDS             <1..471
                     /locus_tag="PKO06_10010"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV70023.1"
                     /translation="RGKLLDALSRKPVPEAELVIDQKIVPLTPDGEFFLEGLRRQQLI
                     EVLITADGYQSLSFKHRVGYKSYLQLLLKPYRASLHLRLISAGTGEPVDGATITLDGK
                     ETPADWTGKASFSGLTPGYQKLVVAAPGYKPATTVVFVEEKTSQRIIKLQPASN"
     gene            599..1117
                     /locus_tag="PKO06_10015"
     CDS             599..1117
                     /locus_tag="PKO06_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70024.1"
                     /translation="MLHRLCLSCLLILALAAPATALNEWTVMLYWVSDDSESTALIKS
                     HISSLKNIVAKASAPNAHDVVVLFDGPRGYTAVDGKDSDGGFRLEAQNGKWSIERLGE
                     VNMGSPYTLWNFIRWAAEKHPAKHYALFIGGHGSGIFSWRGPGGSTISPPEPSTFPPI
                     VSWAMTTLTTTA"
     gene            1018..1857
                     /locus_tag="PKO06_10020"
     CDS             1018..1857
                     /locus_tag="PKO06_10020"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV70025.1"
                     /translation="MARPGWVNDLTPGTVDFSPDRFVGYDDSDNDCLTVFEVQKVLEA
                     FKTKANAGRKLDLLVLDSCLPGGLETLYQLREVISFLVSSPSTTLIGGMPYPRIVSEL
                     AGNVAMSAEEFGKAIAKAYLDKVVTMSNEGEVMAVYTTEKVQELAFALDQLSLQLIAA
                     QTETGKRSSIKNLTTFGGKKRYWDLGRLLKSIAEGNADVGPAKNAAVIKTLAGEALEL
                     MRTNRITTWYSGTYADSKISGLSIAWPEKDEWSKWQKFYQVLDLAKNTHWDEYLGTWH
                     EMR"
     gene            1908..>3394
                     /locus_tag="PKO06_10025"
     CDS             1908..>3394
                     /locus_tag="PKO06_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70026.1"
                     /translation="MNLVRRLFAILILFLLPFCGLLWTIDQIQQQKQQQERTEVIETQ
                     KRILASLTRQASIGYFFREASERIVRALKWDQDPSELISNLPKQTTTLFLFTAQGKRR
                     SGQGFAGDLIAASERCLALILTTARTGETSFTARDRKLASSLLGNENAIILLAQRLGH
                     LVSLEGLGEERSAGCFPFRSVSGRSGYVLMLVNHRHNLDTTLLERSRQRLESLTGKTY
                     TFGWFDLTRLESPATPLPLPGSSTFYSERSTGSRLSDDGSESWCWRVLQRRHLVYSHT
                     RLPPPLQPLAQRHPLLIAMLLAALGILLFLLHRLEERGVSIRLQIIFLFGLSSLASIA
                     ALAGFAVLYLEHRRETLLRDHELTVAETLNQWDSTFQKFCAIRDQNYTQAALQMSQHL
                     SSRERLDAISSQLPGVGTKVSLFLLDHEGRELYRRNSAANMNLHDFLGESYLQVLRLT
                     VKLSCDIERHQRLSKRISNLPEPKTENAFIDALSRDFRGRRGKTRT"
BASE COUNT          710 a         1038 c          912 g          734 t
ORIGIN      
        1 cgcggcaaac tgctggatgc tctcagtcgt aagcctgttc ctgaagctga attggtcatc
       61 gatcagaaga tcgtgccact gactcccgat ggtgagttct ttcttgaggg tttacgtcgt
      121 cagcagttga tcgaggtatt gattaccgct gacgggtacc agagtctgtc attcaagcat
      181 cgtgtcggct acaagtcgta tctgcagctg ctgttaaaac cgtatcgagc cagccttcac
      241 ttgcggctga tttccgccgg taccggcgaa ccggtcgatg gtgcaaccat cacactcgac
      301 ggcaaggaaa cccccgcgga ctggaccggc aaagcctctt tctccggact gacccccggg
      361 tatcagaaac tggtcgtcgc cgcacccggg tacaagcctg ccaccacggt cgtctttgtc
      421 gaggaaaaaa cctcccagcg catcatcaag ctgcaaccag ccagcaactg atcgcgcctc
      481 tctctcgctt cctcttggcg ccatattctt cgcgacgaac accttcgcgc acgttgccca
      541 tcccggggtt ctgacgtatc atacctgtgc caagaattcg tctagaaagg atcgtctcat
      601 gctgcaccgc ctctgcctgt cctgccttct catactggcc ctggccgcac cagcaacagc
      661 cctcaacgag tggaccgtca tgctgtattg ggtcagcgac gactcagaat caacggccct
      721 catcaagtcg cacatttcgt ctctcaaaaa catcgtggcc aaggcgtcag cgcccaacgc
      781 tcacgatgtc gtggttctgt tcgatggtcc gcgcgggtac acggcggttg acggtaaaga
      841 ctccgatggc gggttccgcc tcgaagccca gaacgggaag tggagtatcg agcggttggg
      901 cgaggtcaac atggggtcgc cttacacgct ctggaacttc attcgctggg ccgccgaaaa
      961 acatccggcc aaacactatg ccctgttcat cggcggacac ggcagcggca tcttctcgtg
     1021 gcgcggcccg ggtgggtcaa cgatctcacc cccggaaccg tcgacttttc ccccgatcgt
     1081 ttcgtgggct atgacgactc tgacaacgac tgcctgaccg tattcgaagt tcagaaggtt
     1141 ctcgaggcgt tcaagacaaa ggcaaatgcc ggacgcaaac tggatcttct ggtactcgac
     1201 tcctgcctgc ctggtggtct cgagaccctg taccagttgc gcgaggtcat cagcttcctg
     1261 gtttccagtc catcgaccac gctgatcggc ggcatgccgt atccccgcat cgtcagcgag
     1321 ctggcgggaa atgttgccat gagcgccgaa gagtttggca aagccattgc caaagcctat
     1381 ctcgacaagg tagtcaccat gagcaacgag ggtgaagtga tggccgtcta caccaccgag
     1441 aaagtgcagg aactggcttt cgcactcgat cagttatcgc tgcagctgat cgccgcccag
     1501 acggaaacgg gaaaacgctc cagcatcaag aatctgacca cctttggcgg gaagaagcgt
     1561 tactgggatc tcggccgcct gctgaaatcg atcgcggaag ggaacgccga tgtcggcccc
     1621 gcgaaaaatg cggccgtcat caaaactctt gcaggcgaag ctcttgaact gatgcgcacc
     1681 aatcgcatca cgacctggta ctcaggcacc tatgccgaca gcaagatttc cggactttcc
     1741 attgcctggc cggagaagga cgaatggagc aagtggcaga agttctatca agtgctggat
     1801 ctcgcgaaaa acacgcactg ggacgagtat ctcggcacct ggcacgaaat gcgctgagcg
     1861 gcagtgtctc agcgttctgc ccgtacggtc tcaacgtcgg agcatgaatg aatctggttc
     1921 gccggctgtt tgcgatcctg atcctgtttc tgctgccctt ctgcgggctg ctgtggacca
     1981 tcgatcagat tcagcagcag aaacagcagc aggaacgcac ggaggtcatc gaaactcaga
     2041 agcggattct ggccagtctg acgcggcagg catcgatcgg ctatttcttt cgtgaggcgt
     2101 ctgagcgaat tgtccgagct ctcaaatggg accaggatcc gtctgagctg atcagcaacc
     2161 tgccgaaaca gaccacgacc ctctttctgt tcacggctca gggaaaacga cggtcgggtc
     2221 agggattcgc cggcgatctg atcgccgcct cagagagatg tctcgcactg attcttacca
     2281 ccgcccgcac tggcgaaacc tctttcaccg ctcgagatcg caagctcgcc tcatcgctgc
     2341 tgggaaacga gaatgcgatc attctcctcg cgcagcgtct cggacatctg gtctctcttg
     2401 aagggctggg agaagaaagg tctgccggtt gctttccctt tcgctcggtc agtggtcgtt
     2461 caggatatgt tctcatgctg gtgaaccatc gtcacaacct ggacaccaca cttctggaac
     2521 gatcccgaca acgtcttgag tcactgaccg gaaaaaccta caccttcggc tggttcgacc
     2581 tgacccgcct cgagtcgccc gcgacacccc ttcccctgcc aggatcgagc actttctatt
     2641 ccgagcgttc cacgggttca cgtttgagtg atgatggctc agaaagctgg tgctggcggg
     2701 tactgcagcg ccggcatctg gtctacagtc acacccggct gcccccacca ctgcaacctt
     2761 tggctcaacg gcatccactc ctgattgcca tgttgctggc ggcactgggc attcttctgt
     2821 ttcttcttca ccggctcgag gagcgcgggg tttcgatccg cctgcagatc atctttctct
     2881 tcggtctgtc gagtctggcc agcatcgcgg ctctcgccgg attcgccgtt ctctatctcg
     2941 agcatcgtcg cgaaactctg ttgcgtgacc atgagctgac ggtcgctgaa accttgaacc
     3001 agtgggattc cacgttccag aaattctgtg ccatccggga tcagaattac acccaggccg
     3061 ccctgcaaat gtcgcaacac ctctcgtctc gtgaacgact cgacgccatt tcgtctcagt
     3121 tacccggcgt gggtaccaag gtgtcgctgt ttctcctcga ccacgaagga cgcgaactct
     3181 accggcgcaa ttccgccgcg aacatgaatc tgcacgactt tctcggagaa agttatctgc
     3241 aggtgctgcg gctcaccgtc aagctcagct gcgatatcga gcgccaccag cggctcagca
     3301 agagaatctc caatctgccg gagcccaaaa cggagaacgc tttcatcgat gcgctcagtc
     3361 gtgatttccg gggtcggcga ggcaaaaccc gaac
//
LOCUS       DAORHD010000436         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31392_length_3391_cov_3.119604, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000436 DAORHD010000000
VERSION     DAORHD010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31392_length_3391_cov_3.119604"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PKO06_10030"
     CDS             <1..1630
                     /locus_tag="PKO06_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70027.1"
                     /translation="TSFLVALLCPAPRLAGQSPTDGNGPGNGNDVSLLVFSVEAPLLA
                     GADVRNRIASLCEALLQEPAEAAGPSLLAQLAASGGMYRILSHRQLVQCGIFFPQSFP
                     NVRGWVQTFIAKLQQRWSILPAETGPVSLDDQVYRRLMCERWPRESSQAVSIYVEGAL
                     REGEEELMRQIQRLAVPANSASSTAPDQFVIASMPPSLICLAEWEGPEAEALARAQFA
                     SELVGRAGLPPGVRREVLAFPGRAFLEVIATSTLQNVGDFQALAEEVGRVVNRQDMQD
                     TWREFAALRRQLLQHDRRDFFKGAFINAWEKHFQTSIFEFPASCTWVPPLTFRHWLCL
                     PSEQMHRFMCEVTRKPSVVACRLASGTGVEVAIQISRQTGKAPGATSLRSGLAAFEDL
                     GLSVTVLSDADFLIQAHVEHDQVANLLATVRAVFLEQFLRSEAAGAVKGGQPDLQTDW
                     DVYIAAVGPFPPYMLINSLRKGWPSFGDQNQKIGDPLQMLAAALDIASAAPDLVKGRW
                     QLATSSVYSLTRTLVRYVAKGGSVRELIPLVGPF"
     gene            1718..>3391
                     /locus_tag="PKO06_10035"
     CDS             1718..>3391
                     /locus_tag="PKO06_10035"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV70028.1"
                     /translation="MNVGHRLVCHCTIWTLLLAMLVAMPLFAEDAPAKKKWTFMLYMA
                     ADNNLEGSADADLNELETVGSSDQVNIVVQLDRIGRYSQDSEFKWPEAKRFYVKKDAN
                     LKKFSSPVMQELGEVDMAAPGTLLDFVSWAAHTYPAERYALIFWNHGTGWKEISPQIM
                     AAYDTVDTSRRPGLSPSMEAALNTISYNISYDDTSASSMDIPSLGATIARIPGIIGQP
                     LDLLGFDACLMQMLEVAYEVGEHARYQVGAPDLEPDRGWPYDAIMQKLVAAPTMDGRK
                     LGQTIVSTYKASYEAGTQGNTSVVLSLIDLSKVKGFTSALEGLARALKEGIQEIDSID
                     QAREEALKYVYKDYADLGHFIQLLSVKLKGAAIRSSLAELKTYLQGSVDERLVISCAR
                     TGEKYKNSHGLAVFLPDREGFRTYKNRYKLLAMSKENGWFKFLQEYEAPGFPYICIQD
                     ILLEDENRDGRFAPGELVTVGLQLKNLGTKPATKAHVTLDSASEYLEETKFAINLTEL
                     PAPRKEKIVSVFKFRIKPDAPLNQPVALSASLSGPQIPLSTAHTTFYLKS"
BASE COUNT          700 a          914 c          975 g          802 t
ORIGIN      
        1 gacgagcttt ctcgttgcct tgctgtgccc ggcgcctcgt ctcgccggcc agtcaccgac
       61 cgacggtaac gggccgggga acggtaacga tgtttctctg ctggtttttt ccgttgaggc
      121 tccgttgctg gcaggtgcgg atgtcagaaa tcgcattgcc tctctctgtg aagcgcttct
      181 ccaggagccg gccgaagccg cgggtccctc tttgctggct caattggcgg cctccggtgg
      241 tatgtatcgc attttgagtc accgacaact ggtacaatgc ggtatctttt tccctcaatc
      301 tttcccgaat gtccgtgggt gggtgcagac gttcatcgca aaactgcagc aacgctggtc
      361 gatattgcct gccgaaaccg ggcccgtatc actggatgat caggtgtatc gacggttgat
      421 gtgtgagcgt tggccgaggg agtccagtca agccgtttcc atctatgtgg aaggagcttt
      481 gcgagagggg gaggaggagc tgatgcggca gatccagcgg ttggctgttc ctgcgaattc
      541 ggcttcaagc acggctcctg atcagtttgt tattgcctcg atgccgcctt cgctgatctg
      601 ccttgccgag tgggaaggcc ccgaagcgga ggctttggcg cgtgcgcagt ttgcctctga
      661 attggttgga cgagccggat tgcccccggg cgtgcgtcga gaggtgctgg cttttcccgg
      721 gcgtgcattt ctggaagtga ttgcgacgtc gactttgcag aatgttggtg actttcaggc
      781 tttggccgaa gaagtcggcc gggtggtcaa ccgccaggat atgcaggaca cctggcgtga
      841 gtttgcggca ctgcgccggc aactgttgca gcacgatcgc cgcgacttct tcaagggagc
      901 ttttatcaat gcctgggaaa agcatttcca gacttcgatt tttgagtttc cggcgagctg
      961 tacatgggtt ccaccactga cttttcggca ttggctctgt ctgccgtccg agcagatgca
     1021 tcgtttcatg tgtgaggtga cacgaaaacc ttcggtggtc gcctgccgtc tggcctctgg
     1081 aaccggagtc gaagtagcga ttcagattag tcggcaaaca gggaaagcac ccggagcaac
     1141 cagcttgaga tcgggtctgg cagcgttcga agacttggga ttatcggtca ccgtgttgtc
     1201 cgatgcggat tttctgatcc aggcacatgt cgaacacgat caggtagcca atctgctggc
     1261 gaccgttcgg gccgtctttc ttgaacagtt tctgcgcagt gaagccgcgg gagcggtaaa
     1321 aggggggcaa cccgacctgc aaacggattg ggatgtgtat attgccgcag tgggaccgtt
     1381 tccgccatac atgctgatca acagtttgcg aaaaggatgg ccctcgttcg gcgatcagaa
     1441 ccagaagatc ggcgacccgt tacagatgtt ggccgccgct ctcgatatcg cctctgccgc
     1501 cccggatctg gtgaaaggtc gttggcagct cgcgacttcg agtgtctatt ccctgacacg
     1561 gactctcgtg cgatatgtgg caaagggggg aagcgtgaga gaactgattc ctctggtcgg
     1621 ccctttctga gaccgcctgg acattgacac ttccctgaga cggtcgctat aatcgtacaa
     1681 agcactcgct ttgccacgtc attattggag gccatccgtg aacgttggtc atcgcctcgt
     1741 ctgtcactgc accatctgga cgctcctgct cgccatgctt gtggcgatgc cgctttttgc
     1801 cgaagatgcc cccgccaaga agaaatggac cttcatgctg tacatggcgg cggacaacaa
     1861 cctggaaggt tcggctgacg ccgatctgaa cgaactcgaa accgtcgggt cgagtgatca
     1921 ggtcaacatt gttgttcagc ttgaccgcat tgggcgctac tcccaggatt ccgaattcaa
     1981 atggcccgag gcgaagcgct tctatgtcaa gaaagatgcg aatctcaaga agttttcctc
     2041 gcctgtgatg caggaactcg gtgaagtcga catggcggct cctggcacct tgctcgattt
     2101 tgtgtcctgg gcggcacaca cgtatccggc agaacgatat gcgctcattt tctggaatca
     2161 tggcaccggg tggaaagaga tttctcccca gatcatggct gcctatgata ccgtcgacac
     2221 ttctcggcgg cccggtcttt ccccatccat ggaagcggct ctgaacacta tttcctataa
     2281 catttcctac gacgatacgt cggcgagttc aatggacatt ccctcactgg gagccacgat
     2341 tgcccgcatt cctggcatta tcggccagcc acttgatctg ctgggtttcg atgcctgcct
     2401 catgcagatg ctcgaggtgg cctatgaagt cggcgagcat gcgcgatatc aggtgggagc
     2461 tcctgatctc gagccggatc gcggctggcc atacgatgcc atcatgcaaa agcttgtcgc
     2521 cgcaccgacg atggacggtc gaaaactggg acaaacgatt gtgtcaacct acaaagcttc
     2581 gtatgaagcc ggaactcagg gcaatacctc ggtcgtcctc tctctgattg atctgtcgaa
     2641 agtgaaaggt tttacgtcgg cactcgaagg cctggctcgc gctctgaaag aaggcattca
     2701 ggaaatcgac tcgatcgacc aggctcgcga agaagcgctg aaatacgtct acaaggacta
     2761 tgctgatctc gggcatttca ttcagctgct ctcggtgaaa ctgaaggggg ctgccatacg
     2821 cagttctctt gccgaattga aaacgtacct tcagggcagt gtcgacgagc gtctggtcat
     2881 cagctgcgcc aggaccggtg aaaaatataa gaacagtcat ggtctggcag tctttctccc
     2941 tgatcgtgag ggtttcagaa cgtataagaa ccggtacaaa ctgctggcga tgtccaagga
     3001 gaatgggtgg ttcaaattcc ttcaggaata cgaagcgccc ggtttcccct acatctgcat
     3061 tcaggatatt ctgctcgagg acgagaaccg ggatggtcgt ttcgcgcctg gcgagctggt
     3121 gaccgtgggg ctgcagctga aaaatctcgg aaccaaaccg gcgacaaaag cgcatgtgac
     3181 cctggactcc gcctccgaat atctcgaaga aaccaagttc gcgatcaatc tgaccgagct
     3241 gccagccccg cgcaaagaga agatcgtctc ggtcttcaag tttcgcatca aaccagatgc
     3301 gcctctgaat caacctgtag cgctgtctgc ttcgttgtca ggaccacaga ttccactttc
     3361 gaccgcgcac acgacgttct atctgaagtc t
//
LOCUS       DAORHD010000437         3389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31410_length_3389_cov_4.193761, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000437 DAORHD010000000
VERSION     DAORHD010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31410_length_3389_cov_4.193761"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKO06_10040"
     CDS             <1..367
                     /locus_tag="PKO06_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70029.1"
                     /translation="LDKFESAPIWPYVLMVVALALVLLITRQFDKARTIEPPTWIERP
                     AAEAADDRLPAEAPDPGPLVPTLATPPIPAPLLALSPSSSVIDEAELYNLRVLGLEPT
                     FSSTFGESAMAPWQSSLDS"
     gene            410..1711
                     /locus_tag="PKO06_10045"
     CDS             410..1711
                     /locus_tag="PKO06_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70030.1"
                     /translation="MFSTELTLIVIFALLFGGLPLSIAAAPAAVSPVDPNAAVRYLMA
                     LGWFEPATEKINADLGRLETSTGWKDLDKAAIQYLTGHKMRNVTRLLSLGAACSECNF
                     FPDRKYEPQDHVPPYRRLREMMRTVRGIGIMHQMNGRYQEALDAYSSIYRLGHHLELD
                     GVLIGAVIGISFRKAALACIKDLLAQPVPDSVRQNALGFLKAQRRPAVDLKPLIATEK
                     KFIMKCFEMGKESVEFLAELDLWPDTDPIASQPPSLSSHQKACAANQRVLMGAWEMVT
                     MDYMETATITPVIQEFLVREKYLKNFPVCPEGGTYEFYQEPAGSWAWRCSKHPSPDQV
                     PQSASADTSAPADKVSPERRKKIEAFVASGEFQKLKEAAFATYDEAMALCDHPDKLAS
                     ELVALEERIKTNVILKTVFPSLKKVYENGKAIDDALVELLK"
     gene            1785..1967
                     /locus_tag="PKO06_10050"
     CDS             1785..1967
                     /locus_tag="PKO06_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70031.1"
                     /translation="MIRTLTFSLANRRRFPGFFCFLTDETYRATAGVLNNCTAVAEKL
                     TPMGNGFHGFGFPGHF"
     gene            2025..2402
                     /locus_tag="PKO06_10055"
     CDS             2025..2402
                     /locus_tag="PKO06_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70032.1"
                     /translation="MLKMIKVLLIAVMVLFAAQAFAFNSANLSSAMNTAAYAGEYLNE
                     LMHPGMPKPWTNPMYLTMSNKLADAWKDIKKEIGSIETAKEIDAAWAVVNSFKASQGT
                     YRDLGHQVEISLKERIKFLELQN"
     gene            2655..>3389
                     /locus_tag="PKO06_10060"
     CDS             2655..>3389
                     /locus_tag="PKO06_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNV70033.1"
                     /translation="MMDQHMQTGDQTVAAEAQPLPMGKLLVLGLQHTFTMFGATVLVP
                     LLTGLDVSVALFMAGAGTLVFHLITGGSVPVFLGSSFAFIAPLAAVTGKFGSDAFKPE
                     MMPLALGGACVAGFAYLILAVLVYVYGYRRIVSFFPPVVTGPIIMVIGLKLAPIAVGM
                     AGKNWTLAVISFAIVTAVSVFGKGFLRVLPVLCGLTGGYLAAVLLGYVDFASIQSRGW
                     LAFPNFTLAKFDVQVIMLVAPVAIATM"
BASE COUNT          681 a         1041 c          916 g          751 t
ORIGIN      
        1 cctggacaaa ttcgagtcgg ccccgatctg gccgtatgtt ttgatggtcg tggcactcgc
       61 tctggttctt ctgatcactc ggcagttcga caaggcccga accatcgaac caccgacctg
      121 gatcgaacgc ccggccgcgg aagccgccga cgatcgactg ccggctgaag ctccggaccc
      181 cgggccgttg gttccgactc tggccacccc acccattccg gcccctctcc tcgctctttc
      241 tcccagttca tcggttattg acgaagccga gttgtacaat ctgcgcgttc taggtctgga
      301 accgaccttc tcgtccacct ttggtgagag tgccatggcg ccgtggcagt caagcctcga
      361 ttcctgaagt tctctgctga tcgacaactt acagcaaagg agcatccgca tgttctccac
      421 cgagctcaca ctgatcgtga tcttcgcgct tctcttcggg ggcttgcccc tttccattgc
      481 cgccgcaccg gccgcggtct ctccggtcga ccccaatgcc gcagttcgct acctgatggc
      541 gctcggctgg tttgagccgg ccactgagaa gatcaatgcc gatctcggca gactcgaaac
      601 gagtaccggt tggaaagatc tcgacaaggc agccatccag tatctcaccg gtcataaaat
      661 gcgaaacgtg acgcgcctgc tgtcactcgg agccgcgtgt tccgaatgca acttctttcc
      721 tgatcgaaaa tacgagccgc aggatcatgt tcctccatac cggcgcctgc gcgagatgat
      781 gcgcaccgtt cgcggcattg gcatcatgca ccagatgaac ggtcgctacc aggaagctct
      841 ggatgcatat tccagcattt atcgactcgg acaccatctc gaactcgacg gagttctgat
      901 tggcgcggtc atcggcatca gctttcgcaa ggccgctctg gcgtgcatca aggacctcct
      961 ggcccaaccg gttcccgatt cggtacgtca gaacgcgctc ggctttctca aggcgcagcg
     1021 ccgcccggcg gtcgatctca agcctctgat cgccaccgaa aagaagttca tcatgaagtg
     1081 tttcgagatg ggcaaggagt ctgtcgaatt tctcgccgag ctcgatctct ggcccgacac
     1141 cgacccgatc gccagtcaac cgcccagcct cagcagtcac cagaaggcgt gcgccgcgaa
     1201 tcagcgtgtt ttgatgggcg cgtgggagat ggtcaccatg gactacatgg aaacggcaac
     1261 catcacgcct gtcatccagg agttcctggt cagagaaaag tatctgaaga attttcccgt
     1321 ctgcccggaa ggaggaacct acgagttcta tcaggaacct gccggatcgt gggcgtggcg
     1381 ctgcagcaag cacccctcac cggatcaagt gccgcagagc gcttccgcgg acacgagcgc
     1441 tccggcggac aaagtctcac ccgaacgccg gaagaagatc gaggctttcg tggcctcggg
     1501 agagtttcaa aagctcaaag aagccgcgtt tgccacttat gacgaggcga tggcgctctg
     1561 cgatcatccg gacaaactgg caagcgagct ggtcgctctt gaagagcgta tcaagacgaa
     1621 tgtgattctg aagaccgtct ttccgtcact gaagaaggtg tatgagaacg gaaaagccat
     1681 cgacgacgcc ctggttgaac tgctgaaata atcagctccg acatactttc tgcgccccct
     1741 cgctgctgac gttcttgtca gaacgagggg gttgcgttct cggtatgatc cgcaccctga
     1801 ctttctctct cgcgaatcgc cgacgatttc ccgggttttt ctgctttctg actgatgaga
     1861 cgtatcgcgc gacggcaggc gtgctgaata actgtaccgc cgttgctgaa aagctgacac
     1921 cgatggggaa cggctttcac gggttcggct tcccgggcca tttctgatgt tggcatgaaa
     1981 cctgctgaat gctcgccaca cgaaaacacc aaggagaatg atacatgttg aagatgatca
     2041 aggttcttct catcgccgtc atggttcttt ttgccgctca ggctttcgct tttaactccg
     2101 ccaacctgtc gagcgccatg aacacagccg cttatgccgg tgaatatctg aacgaactca
     2161 tgcatccggg catgcccaaa ccctggacca accccatgta tctgaccatg tcgaacaagc
     2221 tcgccgacgc ctggaaggac atcaagaagg aaatcggttc gatcgaaacc gcgaaggaaa
     2281 tcgacgccgc ctgggccgtg gtcaacagct tcaaagccag ccagggcacc taccgcgatc
     2341 tcggccacca ggtcgaaatc tctctcaagg aacgcatcaa gttcctcgaa ctgcagaact
     2401 agtcgttctc tcagcgaaac cctcaagccg cgccgtcttc accgactgcg cggtttgttc
     2461 ttttcggcag tctggtttcg aacgtgagcc gtctcacacc gaatgactca cacgtttcgc
     2521 acaggccacg aaaaaccggg cgaaaacgac atgaccccgc cgatcacacg aggcaaacga
     2581 ccaggttgag aatggtacgg tgattcggta gactctcact tcgccttacg cacaggcatt
     2641 cttcagggag gatcatgatg gatcagcaca tgcagacagg tgatcagaca gtcgcggcag
     2701 aggctcagcc gttgccgatg gggaagcttc tggttctggg gttacagcac acgttcacca
     2761 tgttcggcgc gaccgtgctc gtgccgttgc tcactgggct cgatgtttcc gtggctctgt
     2821 tcatggcggg tgccggcact ctggtgttcc acctgatcac cggagggagc gtgccggtgt
     2881 ttctcggatc ttcgttcgcg tttatcgcgc cactggcggc cgtcaccggg aagtttggct
     2941 ctgacgcctt caagcctgaa atgatgcctc tggctctcgg cggagcctgc gttgccggtt
     3001 tcgcctactt gatactggct gtgctcgtct atgtctacgg ctaccgccgc atcgtctctt
     3061 tctttcctcc ggtcgtcacc ggccccatca tcatggtcat cggcctgaaa ctggctccga
     3121 tagccgtggg gatggccgga aaaaactgga cgctcgcggt catcagcttc gcgatcgtca
     3181 cggccgtcag cgtcttcggc aaaggctttc tgcgcgtgtt acccgttctc tgtggtctca
     3241 ccggcgggta tctcgccgcc gtcctgctgg gctacgtaga cttcgcttcc attcagtccc
     3301 gcggctggct ggcctttccg aactttactc tggccaagtt cgatgtacaa gtcatcatgc
     3361 tcgtcgctcc ggtcgccatc gcaaccatg
//
LOCUS       DAORHD010000438         3387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31444_length_3387_cov_3.132353, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000438 DAORHD010000000
VERSION     DAORHD010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31444_length_3387_cov_3.132353"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..396
                     /locus_tag="PKO06_10065"
     CDS             19..396
                     /locus_tag="PKO06_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006556519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV70034.1"
                     /translation="MKLNRQAFTLIELMIVIAIIGILAAVAMPHFANAREKARKAKCW
                     EQTSLVSRTCELYYIEQKVYPTQTDLYSSPKFAEYFAGNRVPRCPSHGIDYTLIIGTG
                     NDAVPAKVECYPFHGYASGTWGG"
     gene            complement(460..1137)
                     /locus_tag="PKO06_10070"
     CDS             complement(460..1137)
                     /locus_tag="PKO06_10070"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNV70035.1"
                     /translation="MESRKKQRKRQESFLFLLMGFILLCLLVTGYFVFMDKILPSESA
                     KPVEKPPSVKAPASGGTGEAQTVWAPGSETPSDGTGNDGQLVALYFGARGSEKLVKEM
                     RKIPPEKMALPLAGNLVKEILRGPFTPEARAVVPQGTVLRSIFFHKGTFYVDFSKEFA
                     DNHPGGAMEEALTVFSVVNTLTELDRKARVKILISGMEADSLKGHMGLRQSLCRYEPL
                     FLSATGG"
     gene            complement(1166..2899)
                     /locus_tag="PKO06_10075"
     CDS             complement(1166..2899)
                     /locus_tag="PKO06_10075"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV70036.1"
                     /translation="MNNTFVRVLAFFCFAILVSTAVSAQSVAGKGFVNARELAESMGL
                     NYRWFPMQKALVLSKDGKTMRLEIDQTEGQVGNTVVTLPAAPRLEDGEVMIPARSVVQ
                     VFGAASATPVRPVSRPPEPETDPEPDTDPETETSQPVAPPRVVVPPPAQPAPPAVMQE
                     EEEEEEEVSGTKLVSVRHSLREDHTRVVLEFDGNVTHRLEKVSDNKFKLRIDGCLNII
                     PTKRSNPVGRDVKAISFNSGANRQGLVVSIDVPEGGAIPTVETVGNPFRMVVSVRGGA
                     VTLASPTQLLAATPTVALKPQEPVKPASETVKTPPIAVPEKPASATIKVEKPAEKPPV
                     IEVPVLNASAPSKPVAEEVFEVPLASLSRPVFAGRTIIIDPAHGGRENGVTVPELPSE
                     KQITLNIALKLRQMLKKVGLNAIVLRTQDSDLSQAERQALTNRSAGDLLVSLHCGGST
                     DDLIEGIACFAYDPGGVSFDADAVGKLSPLMVFQEWTQSYRFDLAKFLAGKVRGRLVE
                     QLRMKDRGVRSLPLSPLRFVTMPAVLVEVGMLTHPTEGRRLSGTNFPEAAAKAITNGV
                     LDFFNSIRLNN"
     gene            complement(2896..3387)
                     /gene="smpB"
                     /locus_tag="PKO06_10080"
     CDS             complement(2896..3387)
                     /gene="smpB"
                     /locus_tag="PKO06_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNV70037.1"
                     /translation="MISLKSKAPSNAEGIKIISANRRALHDYFVVEALEVGVALVGSE
                     LRPCREGKVNLQEAYAEVDEQGQLWLFGASIAQNPYSNRMNHDPFRKRRLLAHRKEIL
                     KLGHKVQATGNTLIPLKMYFKKGRVKLEIALAKGKRQYDKRETIAKKDLKRDMDREMS
                     TKR"
BASE COUNT          737 a          989 c          885 g          776 t
ORIGIN      
        1 gaaataggag aactccttat gaaattaaac cgtcaagcgt ttaccctcat tgaactgatg
       61 atcgttattg ccattatcgg tatcctcgct gccgttgcca tgccgcattt tgccaatgcg
      121 cgcgaaaaag cccgcaaagc caaatgctgg gaacagacaa gcctggtgag tcgcacctgc
      181 gagctctatt acatcgaaca gaaggtgtat cccacgcaga ccgaccttta cagcagcccg
      241 aagtttgctg aatattttgc cggcaatcgc gttcccagat gcccttcaca tggtattgac
      301 tacacgctga ttatcggtac cggcaacgat gccgtgccgg ccaaagtgga atgctacccg
      361 tttcacgggt atgcttcagg tacctggggc ggttgatcga cccgccaaga ccctcttgta
      421 agaaaaatcc gggctttggc ccggattttt ttctgcccgc taccctccgg tagcactcag
      481 aaacagcggt tcatagcggc agagcgactg ccgcagcccc atgtgcccct tcagagagtc
      541 cgcttccatt ccactgatga ggatcttcac ccgggccttt cggtctagct cggtcagtgt
      601 gttcacgacc gaaaacacgg tgagagcttc ttccatggct ccacctggat gattgtcagc
      661 gaattctttc gagaaatcga cgtagaatgt tcctttatgg aaaaagatgg agcgcagaac
      721 cgtgccctgg ggcaccaccg cacgcgcctc aggggtgaac ggcccccgca gtatttcttt
      781 gaccagattg ccggcgagcg gaagcgccat tttttccggc ggaatcttgc gcatctcttt
      841 gacgagtttc tcactgccgc gggcaccgaa gtagagtgcg accaactgtc catcgttgcc
      901 ggttccatca gaaggtgtct ctgaccctgg tgcccagact gtctgcgcct cacccgttcc
      961 tcccgatgcg ggcgccttca ccgatggcgg cttctcgaca ggctttgcgc tttcagaggg
     1021 caggatcttg tccatgaaga caaagtaccc ggtgaccagc agacagagca gaataaaacc
     1081 catcaacaga aagagaaagc tttcctgccg ttttcgttgt ttctttcgcg attccatagg
     1141 atcacgcacc tcgatttcgt tcggttcagt tgttcaggcg aatcgaattg aaaaagtcca
     1201 gaactccgtt ggtgatagct ttggcagccg cttctggaaa gttcgtccca gacaggcgcc
     1261 gcccttcggt cggatgcgta agcatcccca cctcgacaag gacggccggc atggtcacaa
     1321 acctcagcgg agacagggga agcgagcgca ctccccgatc tttcatccgc aactgctcaa
     1381 ccagtcgccc acgcaccttg ccggccagga atttggcaag atcgaaccga taactctgcg
     1441 tccattcctg aaaaaccatc aacggggaaa gcttcccgac agcatcagca tcaaagctga
     1501 cgccaccagg gtcataggca aagcaggcaa ttccttcgat cagatcatcc gtcgaaccgc
     1561 cacaatgaag cgaaaccagc aggtcgccgg ctgatcgatt ggtcagagcc tgacgctccg
     1621 cttgagacag atcactatcc tgggttctca gcacgatggc gttcaatcca acctttttca
     1681 gcatctggcg tagtttcaag gcaatgttca aggtgatctg cttttcgctc ggcagttccg
     1741 gcaccgtcac cccgttttct cttccgccat gcgccggatc gataatgatg gttctcccgg
     1801 caaagaccgg acgtgacagg gacgccagag gaacttcaaa cacttcttcg gccacgggct
     1861 ttgacggcgc tgaagcattc agcacgggca cttctatcac tggcggtttt tcggccggtt
     1921 tctcaacttt gatcgtcgcc gaggctggct tttccggaac agcaatcgga ggcgtcttga
     1981 ccgtttctga tgcaggtttt acgggctcct gcggcttgag agcaaccgtc ggggtagccg
     2041 ccaggagttg ggtcggtgaa gcaagagtga ctgccccgcc gcgaaccgaa accaccattc
     2101 ggaaagggtt tcccacggtc tcgaccgtag gaatggcacc accttctggc acatcgatgc
     2161 tcaccacgag accctgacga ttcgccccac tgttgaacga aatggccttc acatcccggc
     2221 caactggatt ggagcgtttg gtggggatga tattgagaca cccgtcaata cgcagcttga
     2281 acttgttgtc gctgactttt tcgaggcggt gggtcacgtt tccgtcaaac tcgagtacga
     2341 ctcgggtgtg gtcttcgcgc agtgaatgcc gaaccgagac cagctttgtt ccagatactt
     2401 cctcctcctc ttcctcctcc tcctgcatga cagcgggggg agcaggctga gccggtggtg
     2461 gaacaactac ccgcggtgga gccacgggct gtgaggtctc cgtttccggg tcggtatccg
     2521 gctctggatc agtttctggc tctggagggc gcgacaccgg gcgaaccgga gtcgcacttg
     2581 ctgccccgaa cacctgcaca acagatcggg ctggaatcat gacttcaccg tcttcaaggc
     2641 gaggcgctgc tggaagggtc acaaccgtgt tcccaacctg accttcggtc tgatcgatct
     2701 cgaggcgcat ggtcttccca tctttgctga ggacgagagc tttctgcatg ggaaaccacc
     2761 gatagttgag ccccatcgac tctgccagtt cgcgggcgtt gacaaaaccc ttaccggcaa
     2821 cggactgagc agaaacagcc gttgacacga gaatggcaaa gcagaagaat gccagcactc
     2881 ggacaaaggt attattcatc gttttgtact catctcacgg tccatgtcac gcttgagatc
     2941 ctttttggcg atggtttcgc gcttatcata ctggcgcttg cctttcgcta acgctatctc
     3001 gagcttgacg cgccccttct tgaaatacat cttcaacgga atcagggtgt tcccggttgc
     3061 ctgaactttg tgtcccaact tgagaatctc tttgcgatgc gccagcaatc tgcgcttgcg
     3121 aaaaggatca tggttcatgc gattcgaata cgggttttgc gcaatactgg cgccgaacag
     3181 ccacaactgc ccctgttcat cgacttccgc gtaggcttcc tgcagattga ccttgccttc
     3241 ccgacaaggg cgcagttccg atccaaccag cgcgacaccg acctcaagag cttcaacgac
     3301 gaaataatcg tgcaaagccc ggcgattggc cgaaatgatc tttatgccct cagcgtttga
     3361 gggtgccttg gatttcaatg aaatcac
//
LOCUS       DAORHD010000439         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31536_length_3381_cov_3.554119, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000439 DAORHD010000000
VERSION     DAORHD010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31536_length_3381_cov_3.554119"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..713)
                     /locus_tag="PKO06_10085"
     CDS             complement(3..713)
                     /locus_tag="PKO06_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV70038.1"
                     /translation="MKIWSIFKRELQSYFYSPVAYIIFSSFLFVVGYLFWVVMVAQKL
                     ATLEPFFGNAAFVLLLISPMLTMRLISEEKRSNSLELLLTSPVSPAEIVMGKFLACFF
                     LYLVLIVVSFKFPAIMYAYAQNLDWGPIISGYIGLVLLSAAFIGLGLFASSLAESQVT
                     AAMVSFAGLILFWIIGWVKFAVEGPIGEAISKLSLIEHYSELVKGVVDSGNIIFFLAF
                     TVVWLFLATRVLESERWR"
     gene            complement(710..1666)
                     /locus_tag="PKO06_10090"
     CDS             complement(710..1666)
                     /locus_tag="PKO06_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015469525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV70039.1"
                     /translation="MIEVKNLTKMYGNLKAVDHLSFEVAKGEILGLLGPNGAGKTTTM
                     RILTCFLPATDGTATVAGYDIFEDSVEVRRRIGYLPETPPLYPEMTVTSYLDFMAGIR
                     EVPYGKVRSRLNDVLERTSLTDHRHRIIGTLSKGYRQRVGLAQALIHDPEVLILDEPT
                     VGLDPNQIIEIRDLIQNLSKDHTVILSTHILSEVQAACGKVAIINEGRLVAVDSVEHL
                     TSGGDVTLFRVALKPATVDWRETFASVPGVELSHVGGEGKHVTFDLRVPNAETYDKVF
                     AAAVAKGHIFTEITPARASLEEVFRRLTVGETAQNGNGGTVQ"
     gene            complement(1962..2726)
                     /locus_tag="PKO06_10095"
     CDS             complement(1962..2726)
                     /locus_tag="PKO06_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70040.1"
                     /translation="MLEFISLRLRNVPRNPEEVFDPFTAEIKALMGEDLISLAVFGSA
                     ASGDYIYGHSNINVVLILKQVTVGLLKKLALPMEKWMTRGFDAPRIFTPQDLERSLDS
                     FPVLFLDIRDNHRVLHGNDLVHQLVIDSGLLRVQTEQLLKKMVADIRTEFLKSGESLK
                     TFESMIARTFNDLYPLLRALLVLKGQKTSVRKEVVVAQAEEVFGLDRGVLVDALRHKM
                     GTIRISELYNLLAYFERYLAAIEKLADAADRLEPIR"
     gene            complement(2889..>3381)
                     /locus_tag="PKO06_10100"
     CDS             complement(2889..>3381)
                     /locus_tag="PKO06_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70041.1"
                     /translation="TVPASEGTAAVPLRELCQIARAMRPDCLLLGETAGAIPAGVLVD
                     AAAGRFGILAAGTASNPEEALECLAATPRSDQDDVADGLARVSLARVFHLVIHQERLS
                     DGSRRITRLSEVFESVDGAHGLRDLFLFEPTGTDENGRIQGSFRSTPALAEFVTHRKR
                     SAV"
BASE COUNT          745 a         1034 c          944 g          658 t
ORIGIN      
        1 acttacctcc agcgttccga ctcgagaacc cgagtggcca gaaacaacca gaccacggtg
       61 aaggccagaa agaagatgat gttgcccgaa tcgacaacgc ctttcaccag ttccgagtaa
      121 tgttcgatca gcgacagctt cgagattgct tcgccgatcg ggccttcgac ggcgaacttc
      181 acccagccga tgatccagaa caggatcaga ccggcaaaac tgaccatggc cgcggtcacc
      241 tggctctcgg ccagactgct cgcgaacaga ccgagcccga tgaaagcggc gctcagcagc
      301 accaggccga tataccccga aatgatcggg ccccagtcga ggttttgggc ataggcatac
      361 atgatcgccg gaaacttgaa cgacacgacg atcaacacca gatacaggaa gaaacaggcc
      421 agaaacttgc ccatgacgat ctcagccggc gaaaccggcg aggtcagaag cagttcgagg
      481 ctgttgctgc gcttttcctc actgatcaga cgcatggtga gcatgggcga gatcagcagc
      541 agcacgaatg cggcgttgcc gaaaaacggc tcgagtgtcg ccagcttctg cgccaccatg
      601 acgacccaga acagatagcc gaccacgaac agaaaactgg aaaagatgat gtaggcgacc
      661 ggcgagtaga agtaactctg caattcacgt ttgaaaatgg accagatctt cattgcaccg
      721 ttcctccgtt gccgttctgc gcggtttcac ccacggtcag gcggcggaag acctcttcga
      781 gcgaggcgcg cgccggcgtg atttcggtga agatgtgtcc cttcgccacg gccgccgcga
      841 acaccttgtc gtaggtctcg gcgttcggca cgcgcagatc aaaggtcacg tgtttgccct
      901 cgccaccaac gtgtgagagc tcgacgcctg gtactgacgc aaaggtttcg cgccagtcga
      961 ccgtcgccgg tttgagcgcg acgcgaaaga gggtgacatc accgcccgaa gtcagatgtt
     1021 cgacactgtc gaccgccacg agccgcccct cattgatgat tgccaccttc ccgcacgctg
     1081 cctgcacttc agagagaatg tgcgtggaga ggatgacggt gtggtctttg gagagattct
     1141 ggatcagatc gcgaatctcg atgatctggt tcggatcgag cccgacggtc ggttcatcga
     1201 gaatcagcac ttcggggtcg tgaatcagcg cctgcgccag accaacccgc tggcgatagc
     1261 ccttcgacag ggttccgata atgcggtgcc gatggtcggt cagactcgtg cgttcaagca
     1321 cgtcgttcag acgcgagcgc accttcccgt agggcacttc acgaatcccg gccatgaagt
     1381 cgagataact ggtgaccgtc atttcggggt acaacggcgg cgtttcgggc agatacccga
     1441 tgcggcggcg aacttcgacc gaatcctcga aaatgtcata cccggcaacg gtagcggtgc
     1501 catcggtcgc gggcagaaag caggtgagaa tccgcatggt cgtggttttc cccgcgccat
     1561 tcggccccag cagaccgaga atctcgccct tcgccacttc gaaggacaga tgatcgaccg
     1621 ctttgaggtt gccgtacatc ttggtgagat ttttcacctc aatcacgcga ttgcctcctg
     1681 atagagttta aaagccgcgg ccacggcggc tttcggttgc ctttgacccg caccgcggaa
     1741 aaaaagttcc aacatttttt gcctcccgac gtcacgtggt cagatcacca aaaacgatct
     1801 cgcgaagcac gaaacctctt cgtttctgac cacatcatca cgatccgcga cagttggtcc
     1861 aatcagaggc acaggatatc gggccccggc ttcccttctc tggtcttgag ggggtcacgc
     1921 agaccgattg gctcacgcca cgccaacgac gcgaatgacc ttcagcgaat cggctccagt
     1981 cggtcagcag catctgccag tttctcgatc gcggcgaggt accgctcaaa ataggccaac
     2041 agattgtaca actcagaaat ccgtatcgtg cccattttat gccgcaacgc atcgaccaga
     2101 actccacgat cgagcccgaa cacttcttcg gcctgggcga cgacgacttc tttgcgcact
     2161 gaggttttct ggcctttcag gaccagcagc gcccgcagca acgggtacaa atcattgaag
     2221 gtgcgcgcaa tcattgactc aaatgttttc agcgactcgc ccgatttcag aaactcggtg
     2281 cgaatgtcgg ccaccatctt tttcagcagc tgttcggtct gaacgcgcag cagccctgaa
     2341 tcaatcacca gctggtgcac cagatcgttt ccatgcagca cgcgatgatt gtcgcgaata
     2401 tccagaaaca gcaccgggaa cgaatccagc gagcgctcga gatcctgtgg cgtgaagatg
     2461 cggggggcat caaaccctct cgtcatccat ttttccatgg gcagggccag cttcttgagc
     2521 aggccgaccg tcacctgttt cagtatcagg acgacgttga tgttcgagtg gccgtaaatg
     2581 tagtcgccag aggccgcgct gccgaacacg gccaggctga tcaggtcttc ccccatgagg
     2641 gctttgatct cagcggtaaa tgggtcgaac acttcctccg ggttgcgagg cacgttccgt
     2701 aggcgaaggc tgataaattc cagcatcgtt caggctcctc tgcgcaagag taaggtctgt
     2761 cttcgggcgg caagtatacc acatcactcg cgagagacaa cccacccccc tgcgaggcag
     2821 ggtcatgtga ttctccggct tgggttttcc ccttcacatc gctcagcgat tcgccgtcgg
     2881 aacccgcctc aaaccgccga acgttttcgg tgggtgacga actctgccaa cgccggcgtc
     2941 gatctgaacg agccctggat ccgcccgttc tcatcggttc ccgtgggttc aaagagaaac
     3001 aggtcgcgca gaccgtgggc cccgtccacc gactcgaata cttcggacag tcgagtgatt
     3061 ctgcgactgc catcggacaa gcgctcctga tgtatcacca gatgaaagac ccgcgccagc
     3121 gacacgcgcg ccagaccgtc cgcgacgtcg tcctgatcgc ttcgcggcgt ggcggccagg
     3181 cattccagag cttcctcggg attcgatgcg gttcctgcgg ccaggatgcc aaatcgtccg
     3241 gcggcggcat ccaccagcac acccgccgga atcgcccctg cagtctcgcc cagcagcagg
     3301 cagtcggggc gcatggcacg agcgatctga cagagttcac gcaggggtac ggctgccgtt
     3361 ccttcagaag ccggcacggt c
//
LOCUS       DAORHD010000440         3377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31599_length_3377_cov_3.590307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000440 DAORHD010000000
VERSION     DAORHD010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31599_length_3377_cov_3.590307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="PKO06_10105"
     CDS             complement(<1..745)
                     /locus_tag="PKO06_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70042.1"
                     /translation="MSTFSPNNSYCSARFTFVLTLLTLVIPCIALAGELNAPAAPNDA
                     GSAMFSIEAIYQRLLNGSTGDKRGGTPGTFKEPAGAPAGTSHTLNDVMGVAPVKDTVD
                     GASPDQVLTGKKFWGLKDGAGWGAQIGQMANHGAMEFTPGAATQTIPQGYYDGNGKVR
                     GDANLVTGNIKAGTTIFGVTGKTEVVDTTSGDATAEEILSGNKAWVGGVEVTGTREPD
                     AFPFSGTRWSNTGLDANGRWCDNGDGTVTD"
     gene            complement(777..2876)
                     /locus_tag="PKO06_10110"
     CDS             complement(777..2876)
                     /locus_tag="PKO06_10110"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNV70043.1"
                     /translation="MKGFSLRLLSVLVFLSFCIVSLIGVSGCRILGSDDSDSGTPNVY
                     QLGGNVFLPEGAANVTAHSTADSLRAAIPAGARPVLNALVWIEGHPEFLTQRTNASGA
                     YLFENLPPGRYRVVVKFAQNGNTNMVRGDETEPTVPSQTPAAVQHLLGLARKFITGTL
                     AGNNGTPLPAGTVVILWGESFPVAEDGTFTAGPLPDGTSDADLTIQQPGQTLSTALTL
                     VVQLFSTDRATTIELQLGGNEQPLLIGATVSALKNNAPVSSANPVSPGDRLNLKATLM
                     NIDPATAGLTYEWNPGRGRFEAAPGNTDTAIWIAPTVPGIATISLKVSAPNRGFCVVR
                     LPITVGSAVDAPKFTVTFNAQGGSAAPSQSIDQGALITKPADPTRTGFRFLGWYKETI
                     GTTLWDFSNDAVNAAVILYAKWVSNDVTTWQVTFNTQGGSDVQSQTIISGEKATLPNP
                     PTKAGFSFSGWYKEVGYLNVWNFDVETVNAAVTLHAQWIADAAPICTLTYAADANGSI
                     TGTNPQLIHQGANGTAVTAVPAAGYQFVKWSDNSTANPRTDTNVTATMTYTASFAAIE
                     GAISGDNKYAWSENSGWINFAPANGGVGSKRGANGYLNGLAWSESLGWIKFAAPGATA
                     PFANNANNTWGVNVAADGMLSGYAWSENSGWINFGATGGNAVMNQTTGELSGFVWSES
                     VGWIHLAGSGYKVQFGS"
     gene            complement(3047..>3377)
                     /locus_tag="PKO06_10115"
     CDS             complement(3047..>3377)
                     /locus_tag="PKO06_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70044.1"
                     /translation="IDPATGSLIGRGEGGEGLSMMEYLQLVKLNLSNLKCCLAFMNSF
                     AGGAGEDAAKDWLMCMTGTDNPGSFVGWAGSVGDIYGVMRHGETLAKIGDCLAGAYEL
                     YGTIKGP"
BASE COUNT          597 a         1000 c         1078 g          702 t
ORIGIN      
        1 gatccgttac cgtgccgtcc ccgttgtcgc accagcggcc gttggcgtct agcccggtat
       61 ttgaccagcg tgttcccgag aaaggaaaag cgtcgggctc acgggttcca gtgacctcga
      121 caccaccgac ccaggcttta tttcctgaaa gaatttcttc tgccgttgcg tcgcccgagg
      181 tcgtgtcgac gacttcggtt ttgccggtga cgccgaagat ggtggtgccg gccttgatgt
      241 tgccagtgac caggttggca tcgccgcgaa ccttgccgtt gccgtcgtaa tagccctgtg
      301 gaatggtctg tgtcgctgct ccgggggtga actccatcgc gccgtggttg gccatctgcc
      361 cgatctgcgc tccccagccg gcgccgtctt tcaggcccca gaacttcttg ccggtcagca
      421 cctggtcggg gctggcacca tcgacggtgt ctttcaccgg agcgacgccc atgacgtcat
      481 tcagcgtgtg cgacgtgccg gcgggcgcgc cggcgggttc cttgaatgtc ccaggcgtgc
      541 cgccgcgttt gtcgcctgtc gagccgttca gcaggcgttg atagatcgcc tcgatcgaga
      601 acatcgcgct gcccgcgtca ttcggcgcgg ccggcgcatt cagctcacca gccagggcga
      661 tacacggaat caccagtgtc agcagtgtaa gaacgaacgt gaaacgggct gaacagtacg
      721 agttgttggg tgagaatgtg ctcatgatat ttctctttcg atgatgaata aggaagttac
      781 gagccgaact gaactttgta ccccgagccg gccaggtgga tccagccgac gctttcggac
      841 cagacgaacc cggacagttc gccggtggtc tgattcatca cggcgttgcc cccggtcgcg
      901 ccgaagttta tccagcccga gttttcgctc catgcgtaac cggaaagcat gccgtcggcg
      961 gcgacgttca cgccccaggt attgttggcg ttgttggcga acggggctgt ggccccggga
     1021 gcggcgaact tgatccagcc caggctttcg ctccaggcca ggccgttgag atacccgttc
     1081 gcgccgcgct tcgagcccac gccaccgttg gctggggcga agttgatcca gccgctgttc
     1141 tcgctccagg catacttgtt gtcgcccgag atcgctcctt cgatcgccgc gaagctggcg
     1201 gtgtaggtca tggtcgccgt gacgttcgta tcggtacgcg ggttggcggt gctgttgtcg
     1261 ctccacttca cgaactgata cccggccgcc ggaaccgcgg tcaccgccgt gccgttcgcg
     1321 ccctgatgaa tcaactgtgg gttggtgccg gtgatcgagc cgttggcgtc ggccgcgtag
     1381 gtcaacgtgc agatcggagc cgcgtcggca atccactgcg catgcagcgt cacggcggcg
     1441 tttacggttt cgacgtcgaa gttccagacg ttgaggtatc cgacttcttt gtaccagccg
     1501 ctgaaggaaa agcccgcctt cgtgggcggg tttggaagcg tagccttttc gcctgagatg
     1561 atcgtctgac tctggacgtc actgccgccc tgcgtgttga acgttacctg ccacgtcgtg
     1621 acatcgttcg acacccactt ggcatacagg atgacggcgg cgttaaccgc gtcgttactg
     1681 aaatcccaga gggtggtgcc gatggtttct ttgtaccagc cgaggaagcg gaatccggtt
     1741 ctggttgggt cggctggttt ggtgatgagc gcgccctggt cgatggattg gcttggggct
     1801 gcactgccgc cctgagcatt gaacgtgacc gtgaatttcg gagcgtcgac ggcagagccg
     1861 acggtgatcg ggagtcgcac cacgcagaaa ccgcggtttg gggcgctgac cttgaggctg
     1921 atcgtggcga tgcccggaac ggtcggcgcg atccagatcg cggtgtcagt gttacccgga
     1981 gcggcttcaa aacgcccgcg acccgggttc cattcgtagg tgagtccggc ggtggccggg
     2041 tcaatgttca tcagcgtggc cttgagattc aaacggtctc cagggctgac cgggttggcg
     2101 gaggagacag gagcgttgtt cttgagcgcg ctgaccgtcg ctccgatcag aagaggctgt
     2161 tcgttgccgc caagctgtag ttcgatggtc gtcgcccggt cggtcgaaaa cagctgaacg
     2221 accagcgtca gggcggtgct cagcgtttgt ccgggttgct gaatcgtgag atcagcatcg
     2281 gacgtgccat cgggcagagg gccggcggtg aaggtgccgt cttcggcgac cgggaacgac
     2341 tctccccaca gaataacgac ggtacctgcg ggcaaggggg tgccgttgtt tccggcgagc
     2401 gtgccggtga tgaacttgcg cgccaggccg agcagatgct gaaccgcggc tggagtctgg
     2461 ctgggaaccg tcggttcggt ttcgtccccg cgaaccatgt tggtgttccc gttctgcgcg
     2521 aacttgacca ccacccgata gcgcccgggc ggaagattct cgaacagata cgcgcccgag
     2581 gcgttggtgc gttgggtgag gaactcggga tgtccttcga tccagacaag ggcgttcagg
     2641 acggggcggg ccccggcggg aatcgccgcc cgcagggagt cggcggtgct gtgggccgtc
     2701 acgttcgcgg cgccttccgg cagaaacacg ttgccgccga gctgatacac gttcggcgtt
     2761 ccgctgtcgc tatcgtcgga gccgagaatg cggcacccgg aaaccccgat cagtgaaaca
     2821 atgcagaacg aaagaaacac caaaacagac aatagacgaa gcgaaaaccc cttcatcaga
     2881 cccgccacat gctcaggatc gcaacgatgc gccgccagtg tcggtcagct gtctgccttg
     2941 tgcaacccga gagaaacgaa actatttctt cacaatatcc catgctatca gttgggtgtg
     3001 gccagatcaa gcccttcttt ggcagtgcga tgtgagtttc cggtcgtcac gggcccttga
     3061 tcgtgccgta gagttcgtac gcaccggcaa ggcagtcgcc gatcttggcg agggtctcgc
     3121 catgcctcat gactccgtag atgtcgccaa cggacccggc ccatcctaca aaactgccgg
     3181 gattatcggt gccggtcata cacatcagcc agtctttggc cgcgtcttcg cctgcaccgc
     3241 cggcaaacga gttcatgaag gccagacagc acttgaggtt agaaaggttc agcttgacca
     3301 gctgtaaata ctccatcatc gacaggcctt cgccgccttc gcctcgacca atgagtgaac
     3361 cggtggccgg gtcgatg
//
LOCUS       DAORHD010000441         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31611_length_3376_cov_4.341765, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000441 DAORHD010000000
VERSION     DAORHD010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31611_length_3376_cov_4.341765"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="PKO06_10120"
     CDS             <1..853
                     /locus_tag="PKO06_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70045.1"
                     /translation="VRKGFKKSLVESMMNQSTPLLMMNDRGNNRPARMNIVTLGMHSE
                     NSQLAGEIKEMLSLGGINTTVAPTKNPFEISLVQTLHGICPYNISAIAEWYSKYRRNH
                     SKINCHAVLANDAYPVLWSSYGLIPTKTLEDLYMVYLLLRYEIFSDDSKTKHGIEYND
                     QLVQFELLYQQEKPQPVGRTIPEIFDYLKNNTMGRYLINNVYKEGWQTMPLPQQNAII
                     KKYMPLFTEKVRSIEDAIEVEIRTNHEVTAASDELLSVKRGILRVLTDKNEEIEAVFS
                     QRPKTAG"
     gene            901..1941
                     /locus_tag="PKO06_10125"
     CDS             901..1941
                     /locus_tag="PKO06_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV70046.1"
                     /translation="MVEVSPKRSEVKLSYRGCLLFLFIIFLLIAVTIWSLLGTQWGAS
                     NSWAEMFRRYLTVVNRFKPGTKPEDNLTSALRIHSLEKLGEKRFALIFSATDKSGDPI
                     VAFSPSDVTVAVGATGQAARPAVIDRVTPLHMMAGWANDKCSFANIMDYSGSMFDHDL
                     RAIESNYSSFISDLVLPFSASVIKFDHRVDEVLPLTENKTDLLSAIGRPGAYGGSTAL
                     YDAMDKGIAQVTARPHFRFVLVATDGNNNQGATSFTDVMARCRSQGISAFVLGFGWLD
                     VNTLKQLANDSDGYYSYVPDSSDLKKWFPRFAKIVNNIQVLEFTTETSAIGLVELSVK
                     AGGTTLKRTRPF"
     gene            1951..>3376
                     /locus_tag="PKO06_10130"
     CDS             1951..>3376
                     /locus_tag="PKO06_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV70047.1"
                     /translation="MPDALIAKAELIADWLDATLLVLREHPRGFADFSPAPLERARFF
                     LRERLLPEVRGGLRLPLVALICGGTNTGKSTIFNALVGRASSPSGGTASLTKRLIASG
                     ADATLQALVQERKDWVLLPIDTLKQPERPDSTLYSIDLTMPAGLPLLIDSPDIDSSDQ
                     TCHRLTRRGLAFADLLIWVTTQQKYRDDAGIAFLDEALSLTATRFDVFNQALSRHQEA
                     LEDMHRTYDSRWPTLERYRFRVDEDRSLSSQDVLAAAAIDPLVVRMREVAREPLTVKI
                     RSLSHGVTCQCELARRQAGEILRRQEEWNKEKTQVQRRLEQHLYTRLRHLRGHDNPLE
                     LQAAMIRVIGPKMQTVVGDALHQGIGVIGRTLGRLRGMIFGSGTRALPEPVDLKAKRD
                     LDDLREAREIMETVRSDLFERSRRKAERGHPLFVRLRQSLQKIAFPQGEELASTLRLH
                     LERESDKRLLPIVTAFEEDLESFCR"
BASE COUNT          757 a          989 c          948 g          682 t
ORIGIN      
        1 agtacgcaaa ggcttcaaga aaagcctggt tgaaagcatg atgaatcagt cgacgcccct
       61 gctgatgatg aacgaccgcg gcaacaatcg tccggcccgc atgaacatcg tcacgctcgg
      121 catgcacagc gaaaactcgc aactcgccgg cgagatcaaa gaaatgctca gtctcggcgg
      181 catcaatacg accgtcgcgc cgacgaagaa ccccttcgag atcagcctgg tgcaaaccct
      241 gcacggcatc tgcccctaca acatcagcgc gattgccgaa tggtattcca agtatcggcg
      301 taatcacagc aagatcaact gtcatgccgt actcgccaat gacgcgtatc cggttctgtg
      361 gtccagttac ggcctgatcc ccaccaagac tctggaagat ttgtacatgg tctatctgtt
      421 gctgcgctac gagatcttct ccgacgacag caagacaaaa cacggaatcg aatacaatga
      481 tcaactggta cagtttgagc tgttgtacca gcaggaaaaa cctcaacccg tcgggagaac
      541 cattcctgag atcttcgact atctcaagaa caacaccatg ggtcgctatc tgatcaacaa
      601 cgtgtacaag gaaggctggc agacgatgcc gttgccacag cagaatgcga tcatcaagaa
      661 gtatatgccg ctgttcaccg agaaagtgcg aagcatagaa gacgccatcg aggtcgagat
      721 tcgcaccaat cacgaggtga ccgcggccag tgatgagctg ctgtcggtca agcgtgggat
      781 tctgcgcgtt ctcaccgaca agaatgagga gatcgaagcc gtcttcagtc agcggcccaa
      841 aacggccggt tgaacgtacc cagacctcaa tcaccaagca tataagggcg gaatcgcaac
      901 atggtcgaag tctcacccaa gcggtcagaa gtcaaactca gctaccgcgg ctgcctgctc
      961 tttctgttca tcatctttct gttgattgcg gtcacgatct ggtcactgct cggcacccag
     1021 tggggcgctt cgaacagctg ggccgagatg ttccgtcgtt acctgacggt cgtcaaccga
     1081 ttcaagccag ggaccaagcc cgaagataat ctgacttccg cgctgcggat tcattcactc
     1141 gaaaaactgg gtgagaaacg ctttgccctg atcttttcgg ccaccgataa gagcggtgat
     1201 ccgattgttg ccttttctcc atctgacgtg accgtggccg tcggcgccac tggtcaggca
     1261 gctcgtccgg cagtgatcga ccgggtgacg ccgttgcata tgatggccgg ctgggccaac
     1321 gacaagtgca gctttgccaa catcatggac tattcaggca gtatgtttga tcacgatcta
     1381 cgggcgatcg agagcaacta cagcagcttc atcagtgatc tggtacttcc gttttcggcg
     1441 tcggtcatca agttcgatca tcgggtcgat gaggttctgc cactgacaga aaacaagacc
     1501 gatctcctga gcgccatcgg cagacccgga gcctacggtg gcagcacggc gctctatgac
     1561 gccatggaca agggcatcgc tcaagtgacc gcgcggccgc acttccggtt cgtgctcgtc
     1621 gctaccgacg ggaacaacaa ccagggagcg acttcgttca ccgacgtgat ggcgcgctgt
     1681 cgcagccagg gtatctcggc tttcgtcctg ggtttcggct ggctcgacgt caacaccctc
     1741 aagcaactgg ccaacgattc agatggctac tactcgtacg ttcccgacag cagcgatctg
     1801 aagaagtggt ttccgcgatt cgcgaaaatc gtgaacaata ttcaggttct cgagtttacg
     1861 acagaaacca gtgccatcgg tctggtcgaa ctttcggtca aggctggcgg cacgacgctg
     1921 aagcggacgc ggcctttcta gccgggagca atgcccgacg cgctgatcgc caaagctgag
     1981 ctgatcgcgg attggctcga tgcgacgctg ctcgtgttgc gcgagcaccc gcgcgggttc
     2041 gccgactttt cccctgcacc gctcgaacgg gcgcgatttt ttctgcggga acggcttctg
     2101 ccggaagtgc gtggtggatt gcgcctgccg ctggtggcgc tcatctgcgg cggcaccaac
     2161 accggcaagt cgacgatctt caatgctctg gtcggccggg caagtagtcc atcaggaggc
     2221 actgccagcc tgaccaagcg cttgatcgcc tcgggcgctg atgcgactct gcaggcgtta
     2281 gtacaagaga gaaaagactg ggtcctgctt ccgatcgata ctctcaagca gccagaacgt
     2341 ccggactcga cgttgtatag catcgacctg accatgccgg ccgggctccc gctgttgatc
     2401 gactctcccg atatcgacag ttccgatcag acgtgtcatc gtctgacacg gcgaggactc
     2461 gcgttcgccg acctgctgat ctgggtcaca acccagcaga agtatcgtga cgacgccggt
     2521 atcgcttttc tggatgaggc cctgagtctc acggccaccc gcttcgacgt cttcaaccag
     2581 gcgctgtcgc ggcatcagga ggctctggaa gacatgcatc gcacctacga cagtcgctgg
     2641 ccgaccctgg aacggtatcg ttttcgtgtc gacgaagacc gctcattgag ctcgcaggat
     2701 gttctcgcag ccgcagcaat cgacccgctc gtcgtccgta tgcgcgaggt tgcccgcgaa
     2761 ccactcacgg taaaaatccg ctctctgagc catggcgtca cctgccagtg tgaattggca
     2821 cgacgacagg ccggagagat tctgcgacga caggaagaat ggaacaaaga gaaaactcag
     2881 gtacagcgcc gacttgaaca acacctgtat acccgtttgc ggcatctgcg cggacacgac
     2941 aacccactcg agcttcaagc cgccatgatc cgggtcatcg ggccgaagat gcagacagtg
     3001 gtcggtgatg cgctgcatca gggcatcgga gtcatcgggc gcacgctcgg tcgtttgcgc
     3061 ggcatgattt ttggcagcgg aacccgtgct cttccagaac ctgtcgacct gaaagcgaaa
     3121 cgcgacctgg acgatctgcg agaagcgcgc gaaatcatgg agaccgttcg ttccgatctg
     3181 ttcgagcgct cgcggcgaaa agcggaacgc ggacatccgt tgtttgttcg tctgcgtcag
     3241 agtctgcaga agatcgcgtt tccgcagggc gaagagctcg cgtcgacgct gcgtctgcat
     3301 ctcgagcgag aatcagataa acggctgctg ccgatcgtca cggcgttcga ggaggacctt
     3361 gaatccttct gccgac
//
LOCUS       DAORHD010000442         3376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31619_length_3376_cov_2.548028, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000442 DAORHD010000000
VERSION     DAORHD010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31619_length_3376_cov_2.548028"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PKO06_10135"
     CDS             complement(<1..545)
                     /locus_tag="PKO06_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HNV70048.1"
                     /translation="MDQASHSKIVSFIWGIADDVLRDLFRRGKYPDVILPMCVLRRLD
                     AVLEPTKKEVLAQKELLDKAGITEQTPALCSAAGQAFYNTSKFVLRDLLSRGSQQQLL
                     ADFTAYLDGFSPNVQEILENFKFRNQIPTLSRADALGTLIAKFLDTDIDLSPKAMDNH
                     AMGTVFEELVRKFNEENNEEAG"
     gene            complement(777..850)
                     /locus_tag="PKO06_10140"
     tRNA            complement(777..850)
                     /locus_tag="PKO06_10140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Arg,seq:ccg)
     gene            1050..2105
                     /locus_tag="PKO06_10145"
     CDS             1050..2105
                     /locus_tag="PKO06_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70049.1"
                     /translation="MMNSLKQVVLVICMFGILLVGGVPALAASGTGDVDGWRHEKLVV
                     SSGKEAWVDLVLPVGTEVILVEAVTSRKADVTLRMFGINVPPLTSGLATLMMVKGYDF
                     KSLPPKNRLRVLADGKAGEQVPIDVYYRGLPHRDAKKPLQLIVDICTLKNAEMRLSAD
                     LMKGVTGYMTEIFRRQGIELSIKTLELPARWAKIHGEENLQKLFAARFGRMTPGAIKI
                     LMVDELEVPEFGLCDSSVAISLGLPAPSSPYLSHLAGVAIPVRDFTSTTAPIDTASAH
                     VGNYIIHELFHALGLPHTMEFDKSRRDSFRSTADNTDSNMMSPMAPRISCPEVTAEQR
                     DYLRQHPLIREPARAGN"
     gene            complement(2235..2828)
                     /locus_tag="PKO06_10150"
     CDS             complement(2235..2828)
                     /locus_tag="PKO06_10150"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNV70050.1"
                     /translation="MLEFLLTVIIGGFIGWSTNVLAVKALFRPIQPRPIGPFDIQGLL
                     PKRRGELAEKLGETIEQNLLSTRELASQVKPSDLEPIVDQMVAEYTHEMRQKVQQDFP
                     NMSFVIQPLISFGGPLVARAIRQRLPEMIERGAEVLVQKVSVKKLVRDKVEQMDLEKL
                     ESVILSVSARELVWIERLGGILGMLVGVLQWGVMRLL"
     gene            complement(2974..>3376)
                     /locus_tag="PKO06_10155"
     CDS             complement(2974..>3376)
                     /locus_tag="PKO06_10155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV70051.1"
                     /translation="DRVLKTVSRLIEKSVREVDIVARYGGEEFVVICPEKDGEGAMIP
                     ANRIRTAIETFDFRINGQPVPITVSLGVCAYPEQARSKPELIMYADTALYYSKESGRN
                     RASHFSPSMRSEEMMKAKAEKKKKKEEEGKH"
BASE COUNT          716 a          901 c         1004 g          755 t
ORIGIN      
        1 cccgcctctt cgttgttctc ttcgttgaac ttgcgtacca gctcttcgaa gacggtgccc
       61 atcgcgtggt tgtccatggc tttcgggctc aggtcgatat cggtgtcgag aaacttggca
      121 atgagcgtac caagagcatc ggcccttgag agcgtcggga tctgattgcg gaacttgaag
      181 ttttcgagaa tctcctgcac gttcggcgag aatccgtcga gatacgcggt gaaatcggcc
      241 agcaactgct gttgactgcc tcgcgacagc agatcgcgca gaacgaactt cgaagtattg
      301 tagaacgcct gcccggcggc actgcacagc gcgggggtct gctcggtgat gcctgccttg
      361 tcaagaagtt ccttctgcgc gagcacctct ttcttggtcg gttcgagcac cgcatcgaga
      421 cggcgtaaca cgcacatcgg cagaatgacg tcggggtatt tgccgcgtcg aaacaggtca
      481 cgcagaacgt cgtcggcgat gccccagatg aaagagacga ttttgctgtg agaggcttga
      541 tccatgaaga ggtaccccga tttctatctg agagtcccgt gtcgttgggt gaaaataccg
      601 accgtaagac gctgtaacaa gaataccacg ctctgaaacc caggtcgaga acctgtgaat
      661 tgtcgcgtat agtgaagaag ccggtaaact cacacaagag cacgaacgtg ttcctttgcc
      721 gaagacaata catgcaaaaa acgggcttca gatgttgatc tgaagcccgt ttgcggcgtg
      781 cccaagagga ttcgaacctc tggcctacag ctccggaggc tgtcgctcta tccagctgag
      841 ctatgggcac cggccatgtg acacggatca taccacatca ccgggtgcga ggcaacgaag
      901 aaacagcccc tgttcacggg gcagtgataa ggtcaaggaa aaagcagtcg ccataagtca
      961 gacacggagt gggtatctga gtatgacagc cgaatctgag gtgtgttaga ttttccctgg
     1021 aagaccacat tccggcaagg atgaactgaa tgatgaattc tctgaagcag gttgttttgg
     1081 tgatctgtat gttcggcatt ctgctggttg ggggcgttcc tgccctcgct gcgagtggga
     1141 ccggcgacgt tgacggctgg cgacacgaaa agttggtggt ttcgtcggga aaggaggctt
     1201 gggtggacct ggttctgccc gttggaaccg aagtgattct ggtcgaggcg gtcacttcca
     1261 ggaaagccga tgtaaccctt cgtatgttcg gtatcaacgt tcccccgctg acctctggcc
     1321 ttgccaccct gatgatggtc aaaggatacg atttcaagag ccttccccca aaaaaccgcc
     1381 ttcgcgtgct ggcagatgga aaggctgggg agcaggttcc catcgatgtc tactatcgag
     1441 ggttgcccca tcgggacgcc aagaaacctc ttcaacttat cgtcgatatc tgtacgttga
     1501 agaatgcaga gatgaggctt tccgctgatc tgatgaaggg agtcacgggg tatatgaccg
     1561 agatcttccg tcgccaggga atagaattgt ccatcaagac tctggaactt cctgcgcgct
     1621 gggcgaagat tcacggcgag gaaaacctgc agaagctttt cgcggcaagg tttggacgaa
     1681 tgaccccggg agccatcaag attctgatgg tggatgaact ggaggttccg gagttcggcc
     1741 tgtgtgattc gagcgtggct atctctctgg gtttgcccgc gccgtcctct ccatacctgt
     1801 cgcatttggc cggggtggcc atcccggtcc gtgatttcac ttccaccacg gccccgatcg
     1861 ataccgcgtc cgctcacgtg ggcaactata tcattcatga gttgttccat gcgctgggtt
     1921 tgcctcacac catggaattc gataaaagcc gacgggacag cttcagaagc acagcggaca
     1981 ataccgattc caacatgatg agtccgatgg cgccccggat ctcttgccct gaagtgacag
     2041 ccgagcagag agactatttg cgtcagcacc cgctgatacg ggaaccagcc cgagccggga
     2101 actgagcggt tcgtggccgt cggagtgccg agaagctcga gagccgatgg atggtgcgga
     2161 tggtgcgtgt gtcgttgctg acgtggtgaa ccgcgatgaa cagcggccgg ccacgcgatc
     2221 tgggaagcgg ctggttacag caggcgcatg acgccccact ggaggacgcc gacgagcatg
     2281 ccgaggatgc cgccgagccg ttcgatccag acgagttcgc gagccgagac ggagagaatc
     2341 acggattcaa gtttctcgag atccatctgt tcgaccttgt cgcgcaccag cttcttcacg
     2401 gagacttttt gtacgagcac ttcggctccg cgttcgatca tttccggcag acgctgccga
     2461 attgcgcggg cgaccagggg accgccaaag ctgatcaacg gctgaatcac gaacgacatg
     2521 ttggggaaat cctgttgtac cttctgtcgc atttcatgcg tgtactcggc caccatctgg
     2581 tcaacaatgg gttcgaggtc tgaaggtttg acctgtgagg ccagttctcg ggtgctgagc
     2641 aggttctgct cgatggtttc ccccagcttc tctgccagct ctccgcgtcg cttcggcagc
     2701 aggccctgga tatcgaaggg gccgatgggg cggggctgaa tcgggcgaaa caaggctttc
     2761 acggccagga cgttggtcga ccagccgatg aatcctccga taatcacggt cagcagaaat
     2821 tcgagcatat tctcttactc atgtgtgaac gaaatcaacg cgtatccacg cccagagttg
     2881 gatacagatc ttgacaactc gtactcgtga ggcgaccggt cagacctgag gcgttgggtg
     2941 gatggggacg ctgaccggta gtcgggccca cgatcagtgt ttgccttcct cttccttctt
     3001 tttcttcttc tcggctttgg ccttcatcat ctcttcgctg cgcatcgaag gcgagaaatg
     3061 tgaagcccgg ttgcggccgg attccttgga atagtacaag gcggtgtcag catacatgat
     3121 caattcaggc ttgctgcgag cctgctcggg gtaagcgcag acgccgagcg acaccgtgat
     3181 cggtacgggt tgcccgttga ttcggaaatc gaaggtttcg atggcggtgc gaatgcggtt
     3241 ggccggaatc atggcccctt caccgtcctt ctcggggcag atgacgacga actcttcgcc
     3301 gccgtagcgg gcgacgatat cgacttcgcg cacgctcttt tcgatgagcc tcgaaacggt
     3361 cttgagcacg cgatcg
//
LOCUS       DAORHD010000443         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31767_length_3365_cov_3.534743, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000443 DAORHD010000000
VERSION     DAORHD010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31767_length_3365_cov_3.534743"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /locus_tag="PKO06_10160"
     CDS             complement(<1..1138)
                     /locus_tag="PKO06_10160"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70052.1"
                     /translation="MQTSVGKTPSPGVYSPLEFLFAGLTAWPNGPFFGLHGALGPLLL
                     GRLAADSRVTLLLTSDEDRARQLHDEARLWLGRHQARAVLFLPDRRSVYDPTPVASEI
                     ALARVAALSLAASGGGIVIAPVRCALERFFQPKRRSGHSLKLQAGASCDRKYLLELLA
                     ELGFERVNLVTEPGQFAVRGSLIDLFPADQEFPVRLDFFGDDLDSIKIFSPETQRTID
                     ALKEFALRPSREQMVTRADIAEILARVDEARLQLDPMRSELLQRRAEHLQTHPDSRDL
                     QELRPFLQPGEGYLWDHWPNPRLLLEQPDQCLEVLDNAWQEIQQIHLNNADVTPLLAP
                     EAYYHPAFAVRDELARLEAQAFSRFQGALSQGCRFHAEPVPAPSL"
     gene            1249..2490
                     /locus_tag="PKO06_10165"
     CDS             1249..2490
                     /locus_tag="PKO06_10165"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV70053.1"
                     /translation="MRRHNVHMRMYLLLNLILIAFLLIVLWRTDPAGAASADLSIRFP
                     TLLLVLLIINTVLFFRDYLRPCLSDQKRSDRAPMMGAARELLSAPVDEPAMIAVLERT
                     IADCRARGACFLSREDADCVAIVARVGMLHASVTTGRILAGASDLRLRHPGGLGEEQI
                     WSASSACALQSCRSRIGRFTLEVMPVRFAGQVVAWFVFEPFDERPVRRHRLEETTVFL
                     QGVGALWFQQRHVATASSLDPVSRLPQFDTFQDALEVELERSERYSQNLTLLYLSVVL
                     PSGGTGTIDDQLLQAAAQSLKDSLRRLDQAFVGEVRGRFAALLTETTPDVARLVADRI
                     QTAFLRRVSQREAGKPAGVALRMGSATYPGDASHAAGLREKADEALQQAVAKGVNFVT
                     YAGMDSPDAPEAKSDGVPYQL"
     gene            2502..2987
                     /locus_tag="PKO06_10170"
     CDS             2502..2987
                     /locus_tag="PKO06_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV70054.1"
                     /translation="MTAQGVPVKCKVNGSMIEIQIPATRTLLEMLRRDLALTGAKEGC
                     GKGECGACTVLLNGQPVNSCLKLAATLLPSDEVETIEGLADDSLMKKLQQSYVDTGAV
                     QCGFCTPGMVMSSYALLRRVARPTLDQIKEAHAGNLCRCTGYRKIFDAVQQAAEKGDR
                     A"
     gene            2984..>3365
                     /locus_tag="PKO06_10175"
     CDS             2984..>3365
                     /locus_tag="PKO06_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNV70055.1"
                     /translation="MTMAFFRPATLREASDVLLQHPRSRIVSGGSDLVVKWKNGVVND
                     LDGLVDIGLLKLNQIECREGVCRIGSGATMTQVASHPLVIERFPALVQAALQVGAMQI
                     RNLATLGGNIANASPAGDTIPALYS"
BASE COUNT          635 a          995 c         1071 g          664 t
ORIGIN      
        1 ccaggctggg ggccggcacc ggctcggcat gaaaacgaca gccttgcgac aaggccccct
       61 gaaagcggct gaaggcctgc gcctcaagtc gagcgagttc gtcgcgcacg gcaaacgccg
      121 ggtgataata ggcttctggg gcaagcagtg gagtgacatc cgcattgttc agatggatct
      181 gctgaatctc ctgccaggcg ttgtcgagca cctcgaggca ctgatcgggc tgttcgagca
      241 acaggcgcgg attcggccag tgatcccaca ggtagccttc tccgggttgc aggaaggggc
      301 gcagctcctg cagatcgcgg ctgtccggat gggtctgcag atgctcggca cgtcgctgca
      361 gcagctcgct gcgcatgggg tcgagctgca ggcgcgcttc atcgacccgc gccagaatct
      421 cggcgatatc agcgcgtgtg accatctgct cgcgagaagg gcgcagcgca aactccttca
      481 gagcatcgat cgttcgctgc gtttccggcg aaaagatttt gatcgaatcg agatcgtcac
      541 caaaaaagtc gagacgaacc ggaaactcct ggtccgcggg gaacaggtcg atcagacttc
      601 cgcgaaccgc aaactgtccg ggttccgtga cgaggttgac ccgctcgaat ccgagctcgg
      661 ccagcagctc caacaggtac ttccgatcgc aggaagcgcc agcctggagt ttcaacgagt
      721 gtccggaacg gcgcttcggc tggaagaacc tttcgagcgc acaacgcacg ggtgcgatca
      781 cgatgccgcc accgctggcg gcaaggctca gagccgcgac gcgtgccagt gcgatctcgc
      841 tggcgacggg agtcgggtca tagaccgagc ggcggtcggg caggaacagc acggcccgcg
      901 cctgatgacg acccaaccaa agacgggcct cgtcgtgcag ttgccgggcg cggtcttcgt
      961 ctgacgttaa cagcagggtc acacggctgt ctgcggcgag gcgaccgagc agcagcggtc
     1021 cgagggctcc atgcaatccg aagaatggac cgttcggcca ggctgtcagc ccggcgaaga
     1081 ggaactcgag cggtgaatac acaccggggg acggtgtctt gcccacagag gtttgcattt
     1141 ccttttcaga actgggtata attccgggac ctgcagaata cgccgtgaca acccggtcgt
     1201 caaggctgca gaactctctg cgacagcagt tccgccaagg aaacgaacat gcgccgacac
     1261 aacgttcaca tgcgaatgta tctgctgctg aacctgattc tgatcgcgtt tctgctgatt
     1321 gtgctgtggc gtaccgaccc cgctggggct gcctcggccg atctgtccat ccggttcccg
     1381 acactgttgc tcgtgctgct gatcatcaac acggttctgt tctttcgcga ttatctgcgg
     1441 ccttgtctgt ccgaccagaa acgctctgat cgagcgccca tgatgggtgc ggcccgcgag
     1501 cttctgtctg ctcctgtcga cgaaccggcg atgattgcgg ttctcgagcg caccatcgca
     1561 gactgccggg ctcgcggagc gtgtttcctc agccgtgaag acgcggactg cgtggcgata
     1621 gtcgcccgtg tcggcatgtt gcacgcatcg gtcaccaccg gccgcattct cgccggagcc
     1681 tctgatctta gactgcgaca cccgggtggg ttgggcgaag agcagatctg gtcagcctcc
     1741 agtgcgtgtg ccttgcagtc gtgccggtcg cggatcggcc gctttactct cgaggtcatg
     1801 cccgtgcgtt ttgccggcca ggtcgtcgcc tggtttgtgt ttgaaccttt cgatgagcga
     1861 ccggtacgcc ggcatcggct tgaggaaacc acggtatttc ttcagggcgt aggcgcactc
     1921 tggtttcagc agcggcatgt cgcaaccgca tcttcacttg atcccgtctc acgtctgccg
     1981 caattcgaca cgttccagga tgcactggaa gtggagctcg aacgttcaga gcgttactcg
     2041 cagaatctga cgctgctgta tctcagcgtg gttctgcctt cggggggcac cggaacgatt
     2101 gacgaccagc tgcttcaggc ggcagcgcag agcctgaaag actcgctgcg ccggcttgac
     2161 caggcgttcg tcggcgaggt tcgcggtcgc tttgcggctc tgctgaccga aacaacgccc
     2221 gatgtggcgc gattggtggc cgaccgcatt cagaccgcgt ttttgcgccg tgtttcgcag
     2281 cgcgaggccg ggaagccggc aggcgtggct ctgagaatgg gctcggcgac ctacccgggt
     2341 gatgcctctc atgcggccgg attgcgtgag aaagccgatg aagcactgca acaggcggtg
     2401 gcaaaagggg tgaatttcgt gacctatgcc ggcatggaca gccccgatgc tcccgaagcc
     2461 aagtccgacg gcgtgcccta tcagctctaa tggatgccat catgacggca cagggtgttc
     2521 ccgtgaagtg caaggtgaat ggctccatga ttgagatcca gattcccgcg actcgtacgc
     2581 tgctcgagat gctacgccga gatctcgctc ttaccggggc gaaagaaggg tgcgggaaag
     2641 gcgaatgcgg ggcctgcacg gtgttgctca acggtcagcc ggtaaacagt tgtttgaagc
     2701 ttgccgcgac cctccttccc tcagatgagg tggagacgat cgaaggtctt gccgatgatt
     2761 ctcttatgaa gaaattgcag caatcttatg tcgacaccgg agccgtgcaa tgtgggttct
     2821 gcactccggg catggtgatg tcttcctatg cgcttctgcg cagggttgca cgcccgaccc
     2881 tcgaccagat caaagaagcc catgccggaa acctctgtcg ctgcaccggg tatcgaaaaa
     2941 tctttgacgc cgtacagcag gccgccgaaa agggagaccg ggcatgacga tggcgttttt
     3001 ccgcccggcc acgctccgcg aagcctccga tgtcctgctg cagcatccgc gatcgcgcat
     3061 tgtgagcggc ggctcagatc tggtggtgaa atggaagaac ggggtcgtga acgacctcga
     3121 cgggctggtc gacattggcc tgctcaagtt gaaccagatc gagtgtcgcg agggtgtgtg
     3181 ccgcatcggt tccggggcga cgatgaccca ggtggcgtcg cacccgctgg tgatcgagcg
     3241 ctttccagcg ctggttcagg ccgcgttgca ggttggcgcg atgcagatca gaaacctggc
     3301 gacgctgggt ggcaacatcg cgaatgcctc gcccgccggc gataccattc ccgcactgta
     3361 tagtc
//
LOCUS       DAORHD010000444         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31791_length_3363_cov_4.346735, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000444 DAORHD010000000
VERSION     DAORHD010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31791_length_3363_cov_4.346735"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKO06_10180"
     CDS             complement(<1..249)
                     /locus_tag="PKO06_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70056.1"
                     /translation="MNTFHDTNRTRCRPTHLRVVLVWGLFLISAWPIHGATLAELIDA
                     ALSRNLGIKRATLQVESARIDEQKARNALIPNLDADLSD"
     gene            complement(246..860)
                     /locus_tag="PKO06_10185"
     CDS             complement(246..860)
                     /locus_tag="PKO06_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV70057.1"
                     /translation="MMKKSPDRPGEFLREGRRLFVSKGFGSTTIEDIVTAVGVGKGTF
                     YSYFRDKADLLLQLIHQSFLELAACTHARRARARTPEQHLEYAFRAQVDYLRQQPDLL
                     NILEQVKIPYFDQINTSFVQAMKHEIAELVQQIEMQQRSGRWRSICPHNLLGVMMVSI
                     HVLLIKELKFGCPVQPSEIRDVLAVYLHGLSIPTPAPAPRRKKP"
     gene            complement(1053..2750)
                     /locus_tag="PKO06_10190"
     CDS             complement(1053..2750)
                     /locus_tag="PKO06_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNV70058.1"
                     /translation="MFSFSWSILGDLSFEQLQKYVGVFLPELTDKPHAEQKRLLQEYL
                     RDADLNPFKAHFAAELLRITGLEKAVPDVYAEFRPVMSDAIRFLLSSIRGERLKEICI
                     EQLLLPPGASPEERLFHLAAGLPTLYKLGQIVARNRHLEPSLRTWLEKLETGYITADF
                     PTLASTLEANLAPYRTDYEWSLEPHLLSEASVGAIVGLHWRRRGEREFHRGVVKLLKP
                     GVRENLDEELALLDQLGDYLEQQRQRYGLTEFPFRDTTRDIADALRRETDFAREQRHL
                     REARRSLGAELGVTVPELLPFCTDRVTAMQRIEGHTLSTCQHSDPEREHLAERLFRAV
                     IGQALFADEPAPLFHGDLHAGNMMTVTRGTSQMPDLFLIDWSQAGRLTRGQREHLMQA
                     AIGMYLKSPERLSRAFVALAEPQQNLSEPLQVRIETIVRNELRESRAAEPVVIRRLFG
                     ICDTLIREGIKFPADLLLFRKTVFSFFDVVASLAPNFNADLCLLHLLAPMIMREFGPR
                     TLESLLPGLLPGNTYRSLVPTPDLVQVGVSTGLAVWRRQMLTACSWWSNTCLGTPRR"
     gene            2937..>3363
                     /locus_tag="PKO06_10195"
     CDS             2937..>3363
                     /locus_tag="PKO06_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70059.1"
                     /translation="MSRSQSLWRAFVGLLVFLYVPMVFLRLLLGMAVSQDEKLRLDEC
                     QDLLSTEMQAYQLDAVPEIQIRNAINGIIGDMPIERSRGVTRRWADELRSRLRGRLPC
                     QYRDFWVFGKDLEPLLIEETPRHMAEKARYFWAYLVQIWD"
BASE COUNT          693 a          946 c         1044 g          680 t
ORIGIN      
        1 atcggaaagg tcggcatcga ggttgggaat cagggcattg cgggctttct gctcatcgat
       61 gcgcgccgac tcgacctgaa gcgtggcgcg cttgatcccg aggtttcgcg acagtgcggc
      121 atcgatcagt tcggcaagcg tggctccgtg aattggccag gcgctgatca ggaacagccc
      181 ccacacgaga acgacgcgga ggtgagtcgg gcggcagcgg gttcgattgg tgtcatgaaa
      241 agtgttcatg gctttttcct tcgtggggcg ggagcagggg tgggaatgga caggccatgc
      301 agatacacgg caagaacatc gcgaatctcg gagggttgaa ccgggcaacc gaacttgagt
      361 tccttgatca gcagcacatg tatactgacc atcatcacgc ccagcaggtt gtgcgggcag
      421 atggaacgcc atctgccgct gcgctgctgc atctctatct gctgcaccag ttcagcaatc
      481 tcgtgcttca tggcctggac aaatgaggtg ttgatctggt cgaagtaggg gatcttgacc
      541 tgttccagga tgttgagcag gtcgggttgc tggcgcaggt agtcgacctg ggcgcgaaat
      601 gcgtattcga gatgctgctc cggtgttcgg gcccgggcac ggcgcgcgtg cgtgcaggcc
      661 gcgagttcca ggaaggactg gtggatcagt tgcagcaaca ggtcggcttt atcccgaaag
      721 taggagtaga acgtgccctt gcccactccg accgcggtga cgatatcctc aatcgtcgtg
      781 ctcccgaacc ctttgctgac gaagaggcga cggccttccc gcaggaattc tccggggcgg
      841 tccggactct tcttcatcat gcctccagcg aatcggtatt ctgaccgact ggtcagtctg
      901 attgcgtggg agcactttat caacatccgg tgcggctgtc aagcagcgca aatcgtgaga
      961 gtccgattgc aggcgggtgc cgagaaatga gactcaggaa gtgacaagtt cgcggttgcg
     1021 tggctgccgg ttttacccaa ccgggcggtc cttcaccggc gaggagttcc caggcaggta
     1081 ttggaccacc aggaacacgc cgtcagcatc tgacggcgcc agacggccag cccggtcgag
     1141 actccaacct gcaccagatc gggagtcggc accagcgatc gatacgtgtt cccgggaagc
     1201 aagcctggta gaagggattc cagggtccgg ggaccgaatt cacgcatgat catcggcgcc
     1261 aggagatgca gcaggcagag atcagcattg aagttcggag ccagcgacgc cacgacgtcg
     1321 aagaacgaaa agacggtctt gcgaaacagc agcaggtcag ccggaaactt gatgccctcg
     1381 cgaatcaagg tgtcgcagat gccgaacaaa cgacgaatga ccactggctc agcggcccga
     1441 ctttccctca actcgttacg gacaatcgtt tcgattctca cctgcagggg ttcggaaaga
     1501 ttctgctgcg gctcggccag ggcgacgaag gctctcgaga gccgctcggg cgatttcaga
     1561 tacatgccaa tcgctgcctg catcaggtgc tcgcgttgac ctctcgtcag gcgacctgcc
     1621 tgactccagt caatgaggaa gaggtcaggc atctgcgacg tgccacgcgt gacggtcatc
     1681 atgttgcctg catgcaagtc gccgtgaaac agcggggccg gctcgtcggc aaacagtgcc
     1741 tgcccgatga cggcgcgaaa caaacgctcg gcgaggtgct ctcgttcggg gtcgctgtgc
     1801 tgacacgtgg aaagcgtgtg tccctcgatt ctctgcatgg cagtcactcg atcggtacag
     1861 aatggcagca gttccggcac ggtgacgccc agttccgccc ccagcgagcg ccgcgcctca
     1921 cgcagatgcc gttgttcccg ggcaaaatcg gtctcgcgcc gcagcgcatc ggcaatatct
     1981 ctggtagtgt cacgaaaggg aaactctgtc agcccgtagc gctgccgttg ctgttcgaga
     2041 taatcgccga gctggtccag aagggcgagc tcttcatcaa gattttcgcg caccccgggt
     2101 ttgagcagct tcacgacccc tcgatggaac tctcgttccc cgcggcgacg ccagtgcaga
     2161 ccgacgatag cgccgacact cgcttccgaa agcagatggg gctcgagact ccactcgtag
     2221 tccgtgcgat aaggcgccag gtttgcttct aaagtactgg ctaatgtcgg aaaatcagct
     2281 gtaatgtagc ccgtctccag cttttcgagc caggtgcgca atgaaggctc gaggtggcgg
     2341 tttctcgcga cgatctgccc cagtttatac aacgtcggca gccccgcggc gagatgaaag
     2401 agccgctcct cgggcgaggc tccgggtggc agcagcagtt gctcgataca gatctccttc
     2461 agacgctcgc cgcgaatcga gctgagaaga aagcgaatgg catcgctcat caccggccga
     2521 aactcggcat atacgtccgg aacggccttc tcgagaccgg taatgcgcag cagttcggcg
     2581 gcaaaatgtg ccttgaacgg gttcaggtca gcatcgcgca gatattcctg cagcagtcgc
     2641 ttctgctcgg cgtgtggctt gtcggtcaac tccggaagaa aaacaccgac gtacttttgc
     2701 agttgctcaa aagagaggtc gccgagaata ctccatgaga acgagaacat gctggactcc
     2761 tgaatgagta ggacatgatg cggcgagaat cagaacgccg catttcgcac ttgcgaagaa
     2821 taccatgttc ttcacctcac gtgcaacttg ggaaaatgcc ccttttcagg tatgattgcc
     2881 gctgccatga gaattctccc gcttcgcctg atcatcagcg cgaccgagac cggtcgatga
     2941 gccgctcgca gagcctgtgg cgagcgtttg tcgggctgct cgtgtttctg tacgtgccca
     3001 tggtgttttt gcgtctgctt cttggcatgg ccgtgagtca ggacgaaaag ctgcgcctgg
     3061 atgagtgcca ggacctgctg agcacggaga tgcaggccta tcagctcgat gcagtgcccg
     3121 agattcagat tcgcaatgcc atcaatggca ttatcggaga catgcccatc gagcgttctc
     3181 gcggggtgac ccgacgctgg gcggacgagt tgcgcagtcg gctgcgcggg cgccttccgt
     3241 gtcagtaccg tgacttctgg gtgtttggca aggatctgga gccgttgctg atcgaggaga
     3301 cgccacggca tatggccgag aaggcgcgct atttctgggc ctacctggtt cagatctggg
     3361 act
//
LOCUS       DAORHD010000445         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31839_length_3360_cov_4.196974, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000445 DAORHD010000000
VERSION     DAORHD010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31839_length_3360_cov_4.196974"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKO06_10200"
     CDS             complement(<1..1023)
                     /locus_tag="PKO06_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNV70060.1"
                     /translation="MAKIFKSFQVKIDKEHHTKVPLKPPAGPTRPPTPEEREAREFQE
                     VPLEALTGRGGLPLSDANAEKSPPPPQPRDEPGSPAESHATFVSAAAKASALIAAANR
                     KWAELNSLEDRLREWESSLAEREKTIASQEGEHQNQFVAKRQHLEEESKKILELARKN
                     SDSLLAQARTEAETIVKAARLEAEEIKKKAHKEGYAIGEEKGIAAGEKAGVEEVQLEW
                     QSLMQETEMLINELQTSRMALLKSSEEEMVRLVLAMARRVIKAESSIKPEIVLRNIDA
                     AINKISDVDKIVLRINMKDKSMAEAHKERLLKRLSGLTELRIIEDPSLAPGGVRIETG
                     VSTIDAS"
     gene            complement(1030..1482)
                     /locus_tag="PKO06_10205"
     CDS             complement(1030..1482)
                     /locus_tag="PKO06_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNV70061.1"
                     /translation="MANTHPNLGRKGIDILADIMQHMQPPKMRHLLKNLKIADPRIVE
                     ELQKRLFTFERLQQCDDRGMQFLLRQISMRDLALALRDAEQPLLEKIANNMSRRSIED
                     LRSEISAIAAARPAEVDAARQRIEQMAKMLIEKNQMYLMQSGKDEFIE"
     gene            complement(1475..2548)
                     /gene="fliG"
                     /locus_tag="PKO06_10210"
     CDS             complement(1475..2548)
                     /gene="fliG"
                     /locus_tag="PKO06_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNV70062.1"
                     /translation="MPEPTTQDKPKDLLKNVPVELGGNKVKLKGKTKAAILLIFLGPE
                     ASTKIFAGLQDTEIEALTIEIARQRKISPEDKMDVLAEFESLMMARQFFAEGGLDYAK
                     VLLEKSLGADKALEILSRMGTSLQVRPFEAARSADPTQLATLIQSEHPQTIALIMSYL
                     QAEKAAIILQSMPMETQTEIAKRLALMDRTSPEVAREVENFLERKLSAVIGQDLTSVG
                     GIDTLVNVLNAVDRQTEKTIIETMEVQEPSLAEEIKKRLFVFEDVVLIDDRGIQRLLK
                     EVDPKDLSISLKGVTDEVKEKFYKNMSKRAAEMLKEEMAYMGPVRIRDVDQAQQRIVS
                     VVKQLEAKGEIIVSRPGEEELIG"
     gene            complement(2569..>3360)
                     /locus_tag="PKO06_10215"
     CDS             complement(2569..>3360)
                     /locus_tag="PKO06_10215"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HNV70063.1"
                     /translation="LSVSVILDANRSQLDSDVEEMIRSTAQATVGFNKKRGDVLSLMV
                     YPFNNDLALRAQRDMEERAKQEKNMFMIVVGLLMAIPVLLGLVYLFVRVSRARALAKE
                     KAALEQAAREAEALRQAEAARKAKLREQQQWEWEQRFQDIKNFFPEITDMEEKKKKVQ
                     ELRHKAYRFARDNDRLPPDFEELTPEEQFLYKEAFKRKAEGTLEDGLARLEAIIGERE
                     KERQEELEKLNAQASQREELESRVRDLAQNRPEDAISVLRIWLEE"
BASE COUNT          572 a          947 c          995 g          846 t
ORIGIN      
        1 cgaggcatcg atggtcgaga cgccggtttc aatgcgaact cctccggggg ccagggaagg
       61 atcttcgatg atacgcagtt cggtcagccc cgagagcctc ttcaagagac gttctttgtg
      121 agcttccgcc atgctcttgt ccttcatgtt gatacgcagc acgatcttgt cgacgtcaga
      181 aatcttgttg atggcggcgt cgatgttgcg cagtacgatt tccggcttga tactggattc
      241 cgccttgatg acccgtcggg ccatggccag caccagacga accatttctt cttcggaaga
      301 cttcagcagc gccatgcgac tggtctgcag ctcgttgatc agcatctcag tctcttgcat
      361 cagagactgc cactcgagct gaacctcttc gacaccggct ttctcaccgg cggcaatgcc
      421 cttttcctcg ccgatggcat agccttcctt gtgtgccttc ttcttgatct cttcagcctc
      481 gagtcgcgcc gctttgacga tcgtttccgc ctcggtgcgc gcctgggcca agagactgtc
      541 actgtttttc cgtgccagtt cgagaatttt cttcgactct tcctcgagat gctggcgctt
      601 ggcgacaaac tgattctgat gctcgccttc ctggctcgcg atggtctttt cacgttcggc
      661 aagactggac tcccactcgc gcagtcgatc ttcgagggag ttcagttcag cccacttgcg
      721 gttggctgcc gcaatgaggg cactggcttt ggccgcagcc gagacgaagg ttgcgtgtga
      781 ctcggcgggg ctcccgggct catcgcgtgg ctgcgggggc ggaggacttt tttcggcgtt
      841 cgcgtcggag agcggcagac cacctctgcc ggtcagggct tcgagcggga cctcctggaa
      901 ctcgcgggcc tcgcgttctt caggcgtggg cgggcgtgtt ggtccggcag gaggtttgag
      961 cggcaccttc gtatggtgct ccttgtcgat cttgacctga aacgatttga aaatcttggc
     1021 cataggcgat tactcgatga actcatcctt tccgctctgc atgaggtaca tctggttctt
     1081 ttcaatcagc atctttgcca tctgctcgat gcgctgtcgg gctgcatcca cttcggcagg
     1141 gcgtgccgcg gcgatggccg agatttcgct gcgcaggtct tcgatcgaac gtcgtgacat
     1201 gttattggcg atcttctcga gcaaaggctg ttccgcgtca cgtaacgcca gagccaggtc
     1261 tcgcatcgag atctggcgaa gcagaaactg catgccgcga tcgtcgcact gctgcagacg
     1321 ttcaaacgta aacaggcgct tctgcagttc ctcgacgata cgcggatcgg cgatcttgag
     1381 gttcttcagc agatgacgca tcttcggcgg ctgcatgtgt tgcatgatat ccgcgagaat
     1441 gtcgattccc ttgcggccca ggttggggtg agtattagcc aatcagttcc tcctctccgg
     1501 ggcgcgaaac gatgatttcg cccttggctt cgagttgttt cacgacggaa acaatgcgtt
     1561 gttgcgcctg gtcgacgtcg cggatacgga cgggacccat atacgccatt tcctccttga
     1621 gcatttcggc cgcgcgcttg gacatgttct tgtagaattt ttccttgacc tcgtcggtga
     1681 cgcctttgag cgagatcgac agatctttcg ggtcgacttc cttgagcagg cgctgaatac
     1741 cacggtcgtc gatgagcacc acatcttcaa agacgaacag gcgcttcttg atttcttcgg
     1801 cgagactggg ttcctgcact tccatggttt cgatgatcgt cttttcggtc tggcgatcga
     1861 cggcgttgag cacgttgacc agggtgtcga taccaccgac cgaggtgaga tcctgaccaa
     1921 tgacggccga gagcttgcgt tcgagaaagt tttcgacctc gcgggcgact tccggactgg
     1981 tgcggtccat cagggcgagc cgtttggcga tttcggtctg ggtttccatt ggcatggact
     2041 gaagaatgat cgcggctttc tcggcttgca ggtaggacat gatcaaggcg atcgtctgcg
     2101 ggtgctcgct ttgaatgagg gtcgcgagct gagtgggatc agcgctgcgc gcggcttcga
     2161 acggccgaac ctgcagcgag gtgcccatgc gtgacagaat ttcgagcgcc ttgtctgcgc
     2221 ccagcgactt ttcgagcagc accttggcat agtcgagacc gccttcggca aagaactggc
     2281 gcgccatcat cagcgactcg aactcggcga gaacgtccat cttgtcttcg gggctgatct
     2341 tgcgctgacg ggcgatttcg atggtcaggg cttcgatctc ggtatcctgc agcccggcga
     2401 agatcttggt actggcttcg gggccgagaa agatcagcag aatggcggcc ttcgttttgc
     2461 ctttcagctt gaccttgttt ccgccaagct cgacgggaac gttcttgagc agatctttcg
     2521 gtttgtcctg ggtggttggt tccggcatgg ttcacctgtc cgagtgggct attcttcgag
     2581 ccagatacgc agtaccgaaa tggcatcttc cgggcggttc tgcgcgaggt cgcgaacgcg
     2641 gctttcgagt tcttcgcgct gcgaggcctg cgcgttgagc ttttcgagtt cttcctgacg
     2701 ttctttttcg cgctcgccga tgatggcttc gaggcgggcc agaccgtctt ccagagttcc
     2761 ttctgccttg cgtttgaacg cttctttgta gaggaactgt tcttcgggag taagctcttc
     2821 aaaatcaggc ggcaagcggt cgttgtcgcg tgcgaaccgg tacgctttat gccgcagctc
     2881 ctgcaccttc ttcttctttt cttccatgtc ggtgatctcg gggaagaagt tcttgatatc
     2941 ctggaagcgc tgttcccatt cccactgctg ctgctcgcgc agcttggcct tgcgcgccgc
     3001 ttcggcctga cgcagtgctt cggcctcgcg tgccgcctgc tcgagcgcgg ctttttcctt
     3061 ggccagagcg cgggcgcgag agacccgcac gaagagatag accaggccca gaagaaccgg
     3121 aatggccatc aggaggccga cgacgatcat gaacatgttt ttctcctgct tggcgcgctc
     3181 ttccatgtca cgctgagcgc gcagagccaa atcgttgttg aacgggtaga ccatgagcga
     3241 cagaacatcg ccgcgctttt tgttgaagcc gacggtggcc tgcgccgtcg agcgaatcat
     3301 ttcctcgacg tcggaatcga gctgagaacg gttggcgtcg agaatgaccg aaacggaaag
//
LOCUS       DAORHD010000446         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31887_length_3357_cov_5.298304, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000446 DAORHD010000000
VERSION     DAORHD010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31887_length_3357_cov_5.298304"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1549
                     /locus_tag="PKO06_10220"
     CDS             <1..1549
                     /locus_tag="PKO06_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV70064.1"
                     /translation="DPVEYNLMGINQVQCHADIGLDFSAALRSFLRQDPNVIMIGEIR
                     DFETASIAIKAAMTGHLVISTLHTNDAPSTIARLMNMGIEPFGITTSIRIVQAQRLVR
                     KICKSCYTEYKPNADLIQTLKVNQKLIDKIGLTKDIDISNLSFAKGAGCPDCDQTGCK
                     GRIGVYEVLSMTQSFREVVERRGTPEELRRQALKDGMLSLRESAMFKLLTKKTTVEEI
                     YRTTMEAGDLDEEALEREIVKKKGKLGAQANVVEGGAGVVASVPEIGGLMKEMEAFRS
                     TLDRIAGGPAKIESISGELLRQNLTDPLTQLQAQITAGMAGQADVSKLLPSIQAQSQK
                     MDFNLRNLTWHFSSLQLKPASLDVAQFLEKNILSQLKQHVLLARILSGKPTIGQGLKI
                     TKNFAPGLPPIAVDAEAFRAVAVNITINSLMAFSQGGELKLVTRLKPQTNNRVEVVFF
                     DNGAGIPSEAQTGLYTPFAPIGRPTLGLGLAVSRKLVQAMNGQISLKSMPGANTLVVL
                     DFPSVSV"
     gene            1637..>3357
                     /locus_tag="PKO06_10225"
     CDS             1637..>3357
                     /locus_tag="PKO06_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70065.1"
                     /translation="MWRSRLFHLRSLVALAFLAAVFVAGVAVAQTPETNVPTGSIELL
                     DEINALAEAKKLEVAQCSQTAKEAGTVRLQISVRAGAHRDFIPFLQGLKKTWIVVPIK
                     FSTRLNSDDRVSSLPILLDLEADLKSGAAAETTTPFEEMLRLTEDIPFFRGADDQPSG
                     KELRLLGMSYRAGGAPELQVLATDLEALTAFTTSLKQTYTFLSLARSQISGRDAYSLR
                     LSLAITGPVIDDFLSLFRTTALQGTLREVTVTPSSDTQPKVLVQFSIPLRRALDLLPM
                     CTIGFSPLVQVNARPDGNGDWNVRALYALKMQPVLVPNQLQWPEIVKVLSEPWPPILY
                     PGMEFEWQLNRMTVTVDYTDQSEFSLLRPLIENGGFRVRHEPVRGQAAERPLRVTLTR
                     SGGVAGDRGEATRPADVDGVPAIGEVVSCEDMGGGAVYTVKLAYADLASFLDGVKPHE
                     HRSIESFQYKQELDGNSWVRLSLVRSTLPGAEAVYETVRCLSRAPFPWKRTEAELAEG
                     LRLTSVSVDAKGRVNVAGQTLKSRRIFSDLFPLLEKTAGLKEPFFKSGTYRDHSVGRV
                     MTFEVTAT"
BASE COUNT          709 a          914 c          987 g          747 t
ORIGIN      
        1 agatccggta gagtacaacc tgatgggcat caatcaggtg cagtgtcatg ccgatatcgg
       61 cctcgatttt tccgccgctc tgcgttcctt tctccgccag gatccgaacg tcatcatgat
      121 cggcgagatt cgcgactttg aaaccgcgag catcgccatc aaagcagcca tgaccggcca
      181 tctggtcatc tccacgctgc acaccaatga cgcaccgtcg acgattgccc gtctcatgaa
      241 catgggcatc gaaccattcg gaatcaccac gtcgattcgc attgttcaag ctcagcgact
      301 ggtacgcaag atctgcaagt cgtgttacac ggaatacaag ccgaacgctg atttgataca
      361 aaccttgaag gtcaatcaga agttgattga caagatcggc ctcaccaaag atatcgacat
      421 cagcaacctg tcgtttgcga agggtgccgg ttgtccagac tgtgatcaga ccggttgcaa
      481 gggacgtatc ggggtctacg aagtgctgtc catgacgcag agttttcgtg aagttgtcga
      541 acgtcgcgga actcctgaag aactgcgccg acaggcactg aaagatggca tgttgtcctt
      601 gcgtgagtcg gcgatgttca agctgctgac caagaagacg accgttgaag agatctatcg
      661 cacgaccatg gaagccggcg atctggatga agaagctctc gaacgcgaga tcgtcaagaa
      721 gaagggcaag ttgggggcgc aggccaatgt cgtggaaggc ggagcgggag tcgttgccag
      781 cgtgccggaa atcggcggcc tgatgaaaga aatggaagcc ttccgctcga cgctcgaccg
      841 cattgccggt ggtccggcca agatcgagag tatcagcggt gaactcctgc ggcagaacct
      901 gacggatccc ctgacgcagt tacaggcgca gatcaccgcg ggaatggccg gtcaggccga
      961 tgtgtcgaaa ctcctcccgt cgattcaggc gcagagccag aaaatggact tcaatctgcg
     1021 caatctgacc tggcacttct cgagccttca gctgaagccg gcatccctcg atgtggccca
     1081 gttcctcgaa aagaacattc tgtcccagtt gaaacagcat gtgttgctgg cccgcatact
     1141 cagcggcaag ccaaccattg gccaggggtt gaagatcacc aagaattttg cgccgggatt
     1201 gccgccgatc gcggttgatg ccgaggcttt tcgagcggtt gccgtgaaca tcaccatcaa
     1261 ctcgctcatg gccttctcgc agggcggcga actcaaactg gttacccgtt tgaaaccgca
     1321 gaccaataat cgcgtcgagg tcgtcttttt tgacaacgga gctggcattc cgagcgaagc
     1381 tcagacaggc ctatacacgc cttttgcccc catcggtcga ccgactcttg gtctggggtt
     1441 ggcggtctca cgcaagctgg tacaggcgat gaatgggcag atctcactca agagtatgcc
     1501 gggtgcgaac acgctcgtgg tccttgactt cccgtccgtg tcggtctgac gacgtctcat
     1561 ccgctcccac tcattttcaa tgtcgacccc gcacgctgat caggggcgaa ggtgcatttg
     1621 accaggaggt tccgctatgt ggcgatcgcg tctgttccat ctgcgctcgt tggttgctct
     1681 tgcgtttctg gctgccgtgt ttgttgctgg tgtggcggtg gcgcaaactc cagagacaaa
     1741 cgtaccaacc ggatcaatcg agctgcttga tgaaatcaat gcattggctg aggcgaagaa
     1801 acttgaggtg gctcagtgca gccagacggc gaaggaagcc gggaccgtac gattgcagat
     1861 cagtgtgcga gccggcgcac atcgcgattt cattcccttt ctgcagggcc tgaagaagac
     1921 ctggatcgtt gttcccatca agttctccac gcgactcaat tccgatgatc gagtttcgtc
     1981 tcttccgata cttctcgatc tggaagctga tctgaagtcg ggtgctgccg ctgaaaccac
     2041 gactcccttc gaggagatgc tgagactgac tgaggatatc ccgttctttc gcggagccga
     2101 tgatcagcca tctgggaaag aactgcgtct gctgggaatg agttatcgtg cgggtggcgc
     2161 tcccgagttg caggtgctcg ccaccgatct cgaggcgctg actgccttca cgacatccct
     2221 gaagcaaacc tacacgtttc tttctctggc tcgcagtcag atctctggtc gcgatgcgta
     2281 ttcgctgcgt ttgtcgcttg cgatcaccgg ccccgtgatc gacgattttc tctcgctgtt
     2341 ccgaacgacg gcacttcagg gaaccctgcg tgaggtcacg gtaaccccaa gctccgatac
     2401 ccagccgaag gtgctggtgc agttctcgat tccgcttcgt cgggccctcg atctgttgcc
     2461 gatgtgtacg atcggtttct cgccacttgt acaagtgaac gccaggccgg atggtaatgg
     2521 cgattggaat gttcgcgcgc tctatgctct caagatgcag ccggttctcg tgccgaatca
     2581 gttgcagtgg cctgagatcg tgaaggtgct gagtgagccc tggccgccca tcctctaccc
     2641 ggggatggaa tttgaatggc aactgaaccg gatgaccgtg acggtcgatt ataccgatca
     2701 gtccgaattc tcgttgctga ggccgttgat cgaaaatggc ggctttcgtg ttcggcatga
     2761 gccggtacgc ggacaggccg ctgagcgtcc attacgtgtg acactcacgc gttcgggcgg
     2821 cgtggccggc gaccggggtg aggcgaccag acctgccgat gtcgatgggg tgccggcgat
     2881 cggcgaggtg gtttcctgcg aggatatggg tggcggggcg gtgtataccg tcaaacttgc
     2941 ctatgccgat ctggcatcat ttctcgatgg cgtgaaaccg catgaacatc gcagcatcga
     3001 atcgtttcag tacaagcagg aacttgatgg aaacagctgg gtaagactgt cactggttcg
     3061 gtcaacgtta cccggagccg aggccgtgta tgaaacggtt cggtgtctga gtcgggcacc
     3121 attcccctgg aaacggacgg aagcggaatt ggcggaaggc ttgcggttga cctcggtttc
     3181 ggtcgacgcg aagggccggg tgaacgtggc cggtcagact ctgaaaagcc gcagaatttt
     3241 cagtgatctt tttcccctgc tggaaaaaac ggccgggttg aaagaaccat tcttcaagag
     3301 tggcacctat cgcgatcact cggtgggtcg cgtcatgacc tttgaagtga cggcaac
//
LOCUS       DAORHD010000447         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_31955_length_3352_cov_4.936306, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000447 DAORHD010000000
VERSION     DAORHD010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31955_length_3352_cov_4.936306"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PKO06_10230"
     CDS             complement(<1..2022)
                     /locus_tag="PKO06_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70066.1"
                     /translation="MRTAFRIACVLMTMVCLAQGAWAVPPSESAKKTLVEAAAGLEKA
                     VERPLAAWQTYKDGNKLEEIGNRYNCVDVADSMRSEYVRVVEAYHAATTNQGDGHDPW
                     PTTDPDIAKAREQAEAVAKKLEAAGMWSAARLGERHDTFVSRLNNMYDQAWVCFLDAK
                     HGKVDEAQKEFKMVDQRFASLKLEAERALASNDRSYDVNFLKSPAMTRFSEALAKFMK
                     DAEPFISQAAGARDATDKDVHAFMELAKKYHEVISNIETKGNLSGSEEEALKRVEEMA
                     VAIEAFEKNDLPGLQKTIDDFKAKYGATSEEIEKKLNEIKGGRHDYNSPGYQFDELQK
                     KIARVKEVRVNMGGQLLESAESEYANSSSFAENIRVKRLEVAKNKLTLAVRLDAGNEK
                     AKQLLAGADAQIASLSAAIEKDIDNREFQGHASSFDGPGNADELAAIAAKYLIKEGWT
                     TNPPRDLLAVRVAGSWRDGDKNLLGEYINYQLGFEAAFHLKTDKEAGKDLARVYYISL
                     YTESRKKEPPFSKSGVGDNYFVRASKVRGIGGSAPTTGSGSGSMAGGGLGSLFWLALV
                     VANLVAGLLAAAPLLNAKAPQLNALYHTITPMRNVLGVAALAIGLLCFVRALILYFAP
                     LADLLPQLAAVVTGLFLGKELLLKKPAETPAPEAPPAAPVAEGAAPAAGP"
     gene            complement(2106..2906)
                     /locus_tag="PKO06_10235"
     CDS             complement(2106..2906)
                     /locus_tag="PKO06_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNV70067.1"
                     /translation="MFYVCSKLFQSVFHAGGFVLLLLLVGCALLYTRWERLGRRLVTL
                     LTVFLLCITLFPVGLWSIAPLEEAFSVPAVLPEKVDGIITMGGSNPWLMAQTNAPALN
                     DDAECLTSFIALARRFPMARLVYTGSTGSIVYPHVSEASAAEILMREQGLDATRFTWE
                     TEARGTAEKAVNTYRLLQPQAGQTWLLLTSAYRMPRTVAVFEAAGWRGRILPYPVDFN
                     APAREYLDFNLEESLKWLWRGMKEWIGLVAYRLSGQSDELFPTQARQR"
     gene            complement(2912..>3352)
                     /locus_tag="PKO06_10240"
     CDS             complement(2912..>3352)
                     /locus_tag="PKO06_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HNV70068.1"
                     /translation="KEEYLNSERYEIKPRDWNKPGNIKTYIAQLNRIRRENPALQEYD
                     NLRFLKAESDHIVFFEKRLGENIVLVAVNLDPFQKQHSFVHVPVEDYGIKANETFQVH
                     DLLVDRRYLWTGSKNYVELTPEEPANVFVIRRWLAREQDFDYFT"
BASE COUNT          662 a         1037 c          913 g          740 t
ORIGIN      
        1 tggtccggcc gccggtgccg cgccttcggc gacgggcgct gccggtggtg cttcaggagc
       61 cggagtctca gccggcttct tcagcagcag ttccttgccg agaaacagcc ccgtcaccac
      121 ggccgccagc tgcggcagca gatcggccag cggggcaaag tagaggataa gtgcacggac
      181 aaagcagagc agaccaatcg ccagggccgc gacccccagc acgttccgca tcggggttat
      241 cgtgtgatac agtgcgttca gttgcggcgc tttcgcgttc agcaggggcg cggcagccaa
      301 tagaccggca accagattgg caaccaccag agcaagccag aagagactgc ccaggccgcc
      361 accggccatg ctgcctgatc cagagccggt cgtcggggcg gaaccgccga taccgcgaac
      421 cttcgaagca cgcacgaagt agttgtcgcc gacaccgctc ttgctgaacg gtggttcctt
      481 cttgcgtgac tcggtataca acgagatgta gtagacacgt gccaggtcct ttccggcttc
      541 cttgtcggtc ttgagatgaa aggcggcctc gaacccgagc tgatagttga tatactcgcc
      601 gagcagattc ttgtcaccgt cgcgccacga cccggccacc cgcaccgcca gcaggtcgcg
      661 cggcggattc gtggtccagc cttctttgat caggtacttc gccgcaatcg ccgccagctc
      721 gtcggcgttt cccggcccgt cgaaactcga ggcatggccc tgaaactcgc gattatcgat
      781 gtccttttcg atggcagcgc tcagagaagc aatctgcgca tctgcccctg ccagcaactg
      841 tttggctttc tcgttgccgg catcgagtcg cacggccaga gtcagtttgt ttttggcgac
      901 ctcgagccgc ttcacccgga tgttctcggc aaaactcgaa ctgttggcat attcgctctc
      961 agcggattca aggagctgac cgcccatatt cacgcgaacc tctttcactc gcgcaatctt
     1021 cttctgaagt tcgtcgaact ggtacccggg gctgttataa tcatgccgcc cgcctttgat
     1081 ctcgttcagt ttcttctcga tctcttcgga cgtggcacca tactttgcct tgaagtcatc
     1141 gatcgtcttc tgcaggccgg gcagatcatt cttttcgaat gcctcgatgg ccaccgccat
     1201 ctcttcgacc cttttcagag cttcttcttc acttcccgaa agatttcctt tggtctcgat
     1261 gttgctgatg acttcatggt actttttcgc cagttccatg aacgcgtgca cgtccttgtc
     1321 agtggcatcg cgcgctccgg ccgcctggga aatgaacggc tcggcatcct tcatgaactt
     1381 ggccagagct tcggaaaatc tggtcatggc cggagatttc agaaagttga cgtcatacga
     1441 gcgatcgttg ctcgccaagg cacgttcggc ttccagcttc aggctcgcga agcgctgatc
     1501 gaccatcttg aattctttct gcgcctcatc gaccttgcca tgcttggcgt cgagaaagca
     1561 gacccaggcc tgatcgtaca tgttattcaa tcggctgaca aaggtgtcat gccgttcacc
     1621 cagacgcgcc gcgctccaca tcccggcagc ttcgagcttt ttcgccaccg cttcggcctg
     1681 ctcacgcgct ttcgcgatat cagggtcggt cgttggccag ggatcgtgac cgtcgccctg
     1741 attggtcgtt gccgcatggt aggcctcaac cactcggaca tactcgcttc tcatggaatc
     1801 ggccacgtca acgcagttgt accggttccc gatctcctcg agcttgttgc catctttgta
     1861 tgtctgccag gcagccaacg ggcgttcgac ggctttttcc aagccggcgg cagcttcgac
     1921 cagagtcttc ttcgccgatt cactgggcgg aactgcccag gccccctgcg ccagacacac
     1981 catcgtcatc agtacacacg cgatacggaa tgcagtcctc acacatcctc ctctgccctg
     2041 cgggcgaaaa gtgaaacgct ggtcctcaca attccggatt atactcaatc gagaatgcga
     2101 gttcatcatc tttgtcgtgc ctgtgtcgga aacagttcgt cggactgccc actcaggcgg
     2161 taggccacca gcccgatcca ctctttcatg ccgcgccaca gccacttgag cgattcttcg
     2221 agattgaaat cgagatactc ccgcgccgga gcattaaaat ccaccggata gggcagaatc
     2281 ctgccccgcc atccggcggc ttcgaacacc gccacggtgc gtggcatgcg ataggccgag
     2341 gtgagcaaca gccaggtctg ccccgcttgc ggctgcaaca accgatacgt gttaaccgct
     2401 ttctcagcgg tgccccgagc ctcggtttcc caggtaaagc gcgtggcatc gagcccctgt
     2461 tcccgcatca gtatttccgc ggctgacgcc tcactgacgt gcgggtagac gatcgacccg
     2521 gtgctgccgg tgtataccag tcgggccatc gggaaacgac gcgccagcgc gatgaagcta
     2581 gtcaggcatt cggcgtcgtc attcaacgcc ggagcatttg tctgtgccat cagccacggg
     2641 ttcgacccac ccatagtgat gatgccatca actttttcgg gcaataccgc cggtacggag
     2701 aacgcttctt cgagtggagc gatgctccag aggccgaccg gaaagagcgt gatacacagc
     2761 agaaacacgg tcagcagcgt gaccagccga cgtcccagtc tttcccatcg ggtgtacaag
     2821 agagcgcacc cgacgagaag caacagaagc acgaatccgc cggcatgaaa taccgactga
     2881 aacagcttcg aacagacata gaacatgccg gtcaggtgaa gtagtcgaaa tcctgctctc
     2941 gcgccagcca acggcgaatc acgaacacat tggcgggttc ttcgggggtc agttcgacat
     3001 agtttttcga gccggtccag aggtagcggc gatcgaccag caggtcatgc acctggaatg
     3061 tttcgttcgc cttgatgcca tagtcctcta cgggaacgtg gacaaaggaa tgctgcttct
     3121 ggaaaggatc aaggttcacg gcaaccagga ctatgttctc gcccaggcgc ttttcgaaga
     3181 agacgatatg gtcgctttcc gctttcagaa agcgcaggtt gtcatactcc tgcagggccg
     3241 ggttctcgcg acgaatgcgg ttgagctgcg cgatataggt cttgatgttg cccggtttgt
     3301 tccagtcacg cggcttgatc tcgtaccttt ccgagttcag atactcttcc tt
//
LOCUS       DAORHD010000448         3346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32053_length_3346_cov_3.386205, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000448 DAORHD010000000
VERSION     DAORHD010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32053_length_3346_cov_3.386205"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="PKO06_10245"
     CDS             <1..1081
                     /locus_tag="PKO06_10245"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNV70069.1"
                     /translation="KEFVFTHLVSAMIPAFFLAAAISTFFSRETLIKTMGSGSNPVLS
                     YPLAALAGGVLTVCSCGILPIFMSLYLGGAGLGPAITFLYASPAINLISLIYTWKILP
                     ISMLWARVISVFTCSMFIGVIMAALFRDRPEAPAEKSESEKKKSVSRTPFQDWCFFGV
                     LVVIMLTSTEAFSGFTQQIIPVSLFSGMTDAQASAWAAFSGKILVIGVELLGLVFLLR
                     AWFTWDETRKWVKRTGRQMKDIIPMVFLGIFYSGMLGGVKNMTEHLGLMSTNSLLANL
                     ASSFIGAILYFGSIVGVNVVDIFRSWGMHDGPALSLLLAGPTISLPSILATLPIIGYR
                     KSLAYAGLVVVFSAACGLIYGSIPH"
     gene            1090..1767
                     /locus_tag="PKO06_10250"
     CDS             1090..1767
                     /locus_tag="PKO06_10250"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV70070.1"
                     /translation="MSSGAQQPPQGNRTFPDSYITLAEEILAPLYSILAETALRSCDD
                     TRGLPVRVLDIGGGTGQWLSALLEKGVSVGILADTDPEMLRHAKTHLGQFLQSNRLLL
                     LHADAQALPFPDASLDLIVSRNSMHLWPNLPQCWKEIFRVLTPGGTAFIGRGYGPDLP
                     GEIRQTIKNQRKQFKQKANEPEIPEPPSPDASHVQSLCLKSGFSRIGIVPDGKAYWIT
                     ARKDSTG"
     gene            complement(1768..2388)
                     /locus_tag="PKO06_10255"
     CDS             complement(1768..2388)
                     /locus_tag="PKO06_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HNV70071.1"
                     /translation="MLSSAEFEAMAERIFEFHRKRAPGIYIGIAMVDRALDELGPVTE
                     KLNVYAETQACLSDVIQIMTGCTTGNRYLRVLKCLGRFAVTVFDRADGRGVRVSVDLT
                     KIDIERMPETSRFFHRQRDPAVQAGGPAREASGMKIMEEFRQFGKELLRVQKVRVRDY
                     GKPPMIAAAVCCDCGESYLKRDEMHVKCDFCSGTTPYFFVEEGRVC"
     gene            complement(2392..3141)
                     /locus_tag="PKO06_10260"
     CDS             complement(2392..3141)
                     /locus_tag="PKO06_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70072.1"
                     /translation="MSNRRLSLAVCFALMCAAASACDVDLEKLLFGGKGGDPFFKAIS
                     QLASYTQSVGQSADDAAVVQERVKTLMNAWIDFDNRYSRKPPEWASGDMQWTEKIKSL
                     TAHIGKLRQSIDVGDSRTVHSLSLELHGRILQLTEASPMVELHRRLLEVSLRFCNVES
                     AVRAKDLVRQKEAVTALASAVRGLAPHVSEKAKLPFQGLLEMIAKVEEAAMTTDEKLR
                     SELKMRAALANDEFRKINRALADTGNSEQKE"
BASE COUNT          720 a          969 c          830 g          827 t
ORIGIN      
        1 gaaggagttc gtcttcactc acctggtgtc agccatgatt ccggctttct ttcttgctgc
       61 tgccatttcg acgtttttct cacgcgagac gctgatcaaa accatgggaa gcggcagcaa
      121 tcccgttctc agttatccgt tggcggctct ggccggggga gtgttgaccg tctgttcatg
      181 tggcattctg ccgatcttca tgagcctcta tctcggagga gctggcctcg gtccggcgat
      241 cacatttctc tatgcgtcgc cggcgatcaa cctgatttcg ctgatctaca cctggaagat
      301 ccttccgatc tcgatgctct gggcccgggt tatcagcgta ttcacttgct ccatgttcat
      361 tggggtcatc atggccgcgt tgtttcgtga tcgtccagaa gcacctgcag aaaagtccga
      421 gtctgagaaa aagaaatcag tgtcgcgaac gccttttcag gactggtgtt tctttggtgt
      481 cttagtcgtc atcatgttga cctcgaccga ggctttttcg ggattcacac aacagatcat
      541 tcctgtctct ctcttttccg gaatgaccga cgctcaagcg tctgcctggg ccgctttctc
      601 ggggaagata ttggtcatcg gcgtcgagtt actgggcttg gtttttctcc tgcgcgcatg
      661 gttcacgtgg gacgaaaccc gtaagtgggt caaacgtacc ggtcgacaga tgaaagacat
      721 cattcccatg gtgtttctcg gcatttttta ctcgggcatg ctgggcggtg tcaagaacat
      781 gacagagcat ctcgggttga tgagtaccaa ctcgcttctc gccaatctgg cctcatcatt
      841 catcggcgcc atcctctact ttggttcgat cgtcggggtc aacgtggtcg acattttccg
      901 atcgtgggga atgcatgatg gtccggctct gtcactcctg cttgctggcc ccacaatcag
      961 cctgccaagt attctcgcca cactaccaat catcggatat cgcaagtcgc tggcatatgc
     1021 cggtctcgtc gtcgtctttt cagcagcctg cggtctcatc tacggcagca ttcctcactg
     1081 agcagaacca tgtcttcagg tgcgcagcag cctccgcaag gcaaccgcac ttttcctgac
     1141 tcgtacatta cacttgccga agagattctc gcccctctgt attccatttt agccgagacc
     1201 gctctgcgct cctgtgacga tacccgtggg cttccagtcc gggtccttga tatcggaggc
     1261 ggcacaggac aatggttgtc cgccttactt gagaaaggcg tgtcagtagg cattctggct
     1321 gacaccgacc cggaaatgct acgccatgcc aagacacatc tgggccaatt tctgcagtcg
     1381 aatcgacttc ttcttctcca tgcggacgca caggctctcc ccttccctga tgcgagtctc
     1441 gacctgatcg tgagcaggaa ctcgatgcac ctgtggccaa atctgccgca atgctggaaa
     1501 gagattttca gggttctgac acctggcgga acggctttca tcggtcgcgg atacgggccg
     1561 gatcttccgg gagagattcg tcagaccatt aagaaccagc gcaagcagtt caagcaaaag
     1621 gccaacgagc cggaaatccc ggaaccacct tcaccagacg cttcgcacgt tcaatcgctg
     1681 tgtctgaaga gcggtttttc ccgtatcggc attgttcctg acggaaaagc ttactggatc
     1741 accgctcgta aagattccac aggataatca gcaaacacgc ccctcctcca cgaaaaagta
     1801 tggagtcgtt cctgagcaga agtcgcattt tacgtgcatt tcatcacgtt tcagatagga
     1861 ctctccacag tcacagcaga cggccgctgc gatcattggt ggctttccgt agtctcgaac
     1921 gcggactttc tggacgcgca gcagttcctt tccaaactgg cgaaattctt ccataatctt
     1981 catccccgaa gcctcgcgcg ccggaccgcc agcctgaacg gccgggtccc gctgacgatg
     2041 gaagaagcga ctggtctccg gcatgcgttc gatgtcaatc tttgtcaggt cgacactgac
     2101 gcgaacaccg cgcccatcgg cacggtcaaa gacggtcacc gcaaagcgac ccagacactt
     2161 caggacacgg agatagcgat tacctgttgt gcaaccagtc atgatctgaa tcacatcaga
     2221 caggcacgcc tgtgtctcag catagacatt cagcttttca gtgaccggcc ccagttcatc
     2281 aagcgcacga tcgaccatgg caattccaat gtagatccca ggggctcgct tacgatgaaa
     2341 ttcgaagatg cgctcggcca tcgcctcaaa ttctgcagaa gaaagcatcg attactcctt
     2401 ttgctcggag ttaccggtat cagcaagagc gcggttgatt tttcgaaact cgtcatttgc
     2461 aagggcagcg cgcatcttga gttcacttcg cagtttttcg tctgtggtca ttgcggcttc
     2521 ttcaaccttg gcaatcattt ccagtagccc ctgaaagggc agcttcgctt tttccgacac
     2581 atggggggct aatccgcgca cggcactcgc aagggctgtc acagcttctt tctgccgaac
     2641 cagatccttg gctctcacgg cagattcgac gttacaaaac cgcagcgaaa cttccagcaa
     2701 tctgcgatgc aactcaacca tcggactcgc ctctgtcagt tgcagaatcc ttccatgcaa
     2761 ctcgagagac aaagagtgca ccgtacgcga atctccaaca tcaatgcttt gccgaagttt
     2821 cccaatgtgt gctgtcaggg attttatctt ctccgtccat tgcatatcgc cgctcgccca
     2881 ttccggcggt ttacggctgt accggttatc aaagtcaatc caggcgttca tgagtgtttt
     2941 cacgcgttcc tgaacgaccg cagcgtcatc agcagactgc ccgacacttt gtgtgtaaga
     3001 agccagttga gatattgcct tgaagaatgg gtctcccccc ttccccccga agagcaactt
     3061 ttcgagatcg acatcgcaag ccgaggccgc agcacacatc aatgcgaagc acacggccag
     3121 ggatagtctt cggtttgaca tggttctctc ccagagtggc gttcctttct ttcgttttac
     3181 cacaaacgag ctcagaatgt cagaagtcgt ccaagctcag tgtagtacag caacgcgctc
     3241 gataccggga acatcctacc tgaatacgat cagcagcagc atgaggaaag cggtaccggc
     3301 cggaataacc caaatcgcat gacggtcgat tttccgtgcg gtctcg
//
LOCUS       DAORHD010000449         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32149_length_3338_cov_3.773073, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000449 DAORHD010000000
VERSION     DAORHD010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32149_length_3338_cov_3.773073"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="PKO06_10265"
     CDS             <1..1576
                     /locus_tag="PKO06_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70073.1"
                     /translation="RIKIDEVTADIDAYDPVTGLVRFTPRTSLGEGVHIAIFYAYDLA
                     GNAANPAAVSFEVAFPKTKDTEGREVAFRDRHVPTITELEPAEGTTVYSANPVISCRL
                     FDAESGIDPQTISFWLNDEKIANHVTYYIPGRTGEDWDWWSYQRDVILYNPLSGKVMF
                     SPVKPLDEKTAWYSFSYEACDRQGNPVRSPTIKFKVVIDRQPPSISAFEPADQAVLTS
                     LTPAIKAQISDKGGSGVSKRDLRLFFDGDRISTEDLDYDEKTGLLSWVPPLAVDPDQQ
                     HIVSLIVFDRAGNRSPESVATFFVRPDTQAPILSSAQPRSGQVISTRTGSLYLRWLDP
                     GFSGLDDTTTRIALDGRAVLPDNPDTSFIDGYRLKFEDLSGKRVARVEIPISGAEALG
                     QGTHQLIVSIADRAGNRSSPEVIDFRVSDDITAPVITALTPENISTIAPTAIRVSASF
                     TDEGSGIDLNRVILALNGQRIAISPEHLASGGQLSLTIMPSQPLEKQSLHLLSLTVYD
                     LAGNRSESRVHVLRCN"
     gene            complement(1599..>3338)
                     /locus_tag="PKO06_10270"
     CDS             complement(1599..>3338)
                     /locus_tag="PKO06_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV70074.1"
                     /translation="SGPVSFSLTLMHTNDLHSHLLGTGPDNRFTPQVGDGDPVRGHFG
                     RLATVMREIASQAQTAGEPCLIFDSGDSYCGTLFQLLGPATATTRLPEYEFFTDLGYT
                     ALGLGNHEFEAGESGLNVMLGKMRRYKPMVPIVATNLILGRSAGVLRAHWNPDANPAS
                     GGVLMPYLIHACQVGTQTLHVGILGALGPDAAKLSLANRSEAHFVGFDDDAGKADLGA
                     LVAFLQERVSRLREQHQVDLVILLLHGGREEETAIAQGVTGIDLILAGHTHETYVRQE
                     GKTWISQSGWRGENLNVLALSREPSGLNLRHAETAVRAVDDTIVAATDQMAKFREYES
                     TLDQMIHPSGYSVLEPVCLIDRERRRERWPNNQAGMLVVDALKTGVNARLIKPVHVYV
                     SSYAMIRSDFLTPGGVPTVYQFSDIFRFSPLGHDPGGRGGAPVTVFHLTPKEVHLVLE
                     AMHWFSRHTPAYEPVVSSGLTYELSMWGVPLVNRVHSLKLDGVPFESWPPLIRVAANE
                     FLTRNLLRIRSISGGMVDVSLRDEEGRPVTSLPSLALPPEHEILAHQLRQQFPIPASG
                     PISLPASLTIPMR"
BASE COUNT          720 a         1045 c          939 g          634 t
ORIGIN      
        1 tcggatcaag atcgacgaag tgacggcgga catcgatgcg tatgatcccg tcactggcct
       61 ggtccgcttt actccccgca cttcgctggg cgaaggcgtg catatcgcga tcttctatgc
      121 gtatgacctg gcggggaatg ccgccaaccc ggcggcggta agcttcgaag tcgcttttcc
      181 gaaaaccaaa gacaccgagg gacgcgaagt cgcctttcgc gatcgtcacg tgccgacgat
      241 caccgagctc gaacccgcgg agggaaccac cgtctacagc gcgaatccgg tgatttcgtg
      301 tcgattgttc gacgcggagt caggcatcga cccacagacc atcagcttct ggctcaacga
      361 cgagaagatc gccaaccacg tcacctacta cattcccggc cgaaccggcg aagactggga
      421 ctggtggagc taccagcgcg atgtcatctt gtacaatccg ctcagcggca aggtgatgtt
      481 cagcccggtc aagccactcg acgagaaaac cgcctggtac tcgttctcct atgaagcctg
      541 cgatcggcaa ggcaacccag tacgatcgcc gacaatcaag ttcaaagtcg tcattgaccg
      601 gcagcccccc tcgatctccg cgttcgaacc tgccgaccag gccgtgttga cctcactgac
      661 tccagccatc aaggctcaga tcagtgacaa gggcggcagc ggcgtcagca agcgtgacct
      721 gcgcctgttc ttcgacggcg accgcatctc aaccgaagac ctcgactacg atgagaagac
      781 gggtctgttg tcctgggtgc cgcctctcgc ggtcgatccc gaccagcagc acatcgtctc
      841 cctcattgtt ttcgatcgag ccggcaaccg gtctcccgaa tccgtcgcca cgttcttcgt
      901 gcgcccggat acgcaggcgc cgatactgtc ctcggctcaa cctcgttcgg ggcaagtgat
      961 cagcacccgc accggcagcc tgtatctgcg ctggctcgac ccgggcttct cgggactcga
     1021 tgatacaaca acccgcattg cgcttgatgg gcgtgcggtc ttgcccgaca acccggacac
     1081 ttcattcatc gatggctatc gactgaagtt cgaagatctc tcaggaaaac gcgtcgcccg
     1141 cgttgaaatt ccgatcagcg gcgcagaagc tctggggcaa gggacgcacc aactgatcgt
     1201 gtcgattgcc gatcgggccg ggaaccgcag cagtcctgaa gtcatcgact tccgcgtttc
     1261 cgacgatatc acggcccccg tgattaccgc cctgactccg gaaaacatct cgacgattgc
     1321 cccgacggcc atccgcgtct cggcctcatt cacggacgaa ggttccggca tcgacctgaa
     1381 tcgggtcatt ctcgccttga atgggcaacg catcgcgatc tcgcctgaac atctggcttc
     1441 gggtgggcag ctgtctctca ccatcatgcc ctctcagccg ctcgagaagc agagcctgca
     1501 cctgttgtcg ctgacggtct atgacctggc agggaaccgt tcagaatctc gcgtccacgt
     1561 gctgcgctgc aattgaccct gatgcagtgt cgcagacctc agcgcatcgg aatcgtgagt
     1621 gatgcaggca gagagatcgg cccggaagcc ggaatgggaa actgctgtcg cagctggtgc
     1681 gcgagaatct cgtgttcggg cggaagagca agtgacggca gggatgtcac cggccgtcct
     1741 tcttcgtcac gcagtgacac atcgaccatg ccgcccgaga tgctgcgaat gcgcaggaga
     1801 ttccgcgtca gaaattcgtt ggcggccacg cgaatcaagg gaggccatga ttcgaatggc
     1861 acgccatcga gtttgagaga gtgaacccgg ttcaccagag gcacgcccca catactcaac
     1921 tcgtaggtca gacccgatga cacgacgggc tcgtacgccg gggtgtggcg cgaaaaccag
     1981 tgcatggctt cgagaaccag atgcacttct ttcggagtca ggtgaaacac cgtcaccgga
     2041 gcgccacctc tgccaccggg atcatgtccg agcgggctaa aacgaaagat gtcggaaaac
     2101 tgatacaccg tgggaacgcc tcccggcgtc aggaaatcag aacgaatcat ggcatacgaa
     2161 gaaacataga cgtgaaccgg ctttatcagg cgtgcgttca ccccggtctt cagggcgtcg
     2221 acgacgagca ttccggcctg attgttcggc cagcgctcac gccggcgttc gcgatcaatc
     2281 aggcagaccg gttctagaac ggaatacccg cttggatgga tcatctgatc aagagtcgac
     2341 tcgtactctc gaaatttggc catctgatca gtggcggcga caatggtatc atcgacagcc
     2401 cgaaccgccg tttccgcatg tctcagattc agacctgacg gttctcgact caaggcaagt
     2461 acgttgagat tctctccgcg ccagccactt tgagagatcc aggtttttcc ttcctgccga
     2521 acatacgtct catgcgtgtg cccggccagg atgagatcga tgcccgtcac gccctgtgca
     2581 atcgcggtct cttcctcgcg ccccccgtgc agcagcagaa tcaccaggtc gacctgatgc
     2641 tgctcgcgca gacgcgagac gcgctcctgc aggaaggcaa ccagtgcgcc cagatcagcc
     2701 ttgccagcgt catcatcgaa cccgacgaaa tgcgcttcgg atcgattggc gagcgacagc
     2761 ttggcggcat ccggtcccag cgcgcccaga attccgacgt gcagggtttg tgtgccgacc
     2821 tgacaggcat gaatgaggta tggcatcaac accccaccgg aggcggggtt cgcatccgga
     2881 ttccagtgag ctcgcaacac cccggccgaa cgccccagaa tcagattggt cgccacaatc
     2941 ggcaccatcg gtttgtaccg gcgcatttta ccgagcatga cgttcagacc gctctctccg
     3001 gcttcaaact catgatttcc caaccccaga gccgtgtatc ccagatcggt gaaaaactcg
     3061 tattccggaa gtcgtgtcgt cgccgtcgct ggacccagaa gctggaagag agttccgcaa
     3121 taagagtcac ccgaatcaaa gatcagacaa ggttctccgg cagtctgcgc ctgcgaggca
     3181 atttcgcgca tgaccgtggc gagtcgtccg aaatgaccgc gcaccgggtc accgtcaccc
     3241 acctgcggcg taaaacgatt gtcgggcccg gtacccaaca ggtgcgaatg cagatcattg
     3301 gtgtgcatga gggtcaggga gaacgaaacc ggaccgga
//
LOCUS       DAORHD010000450         3324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32364_length_3324_cov_4.008259, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000450 DAORHD010000000
VERSION     DAORHD010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32364_length_3324_cov_4.008259"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKO06_10275"
     CDS             <1..560
                     /locus_tag="PKO06_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70075.1"
                     /translation="LIFAGYPKAQNNLGLIYLDGRPGVPANIKLAHKYLGMAAEQKYQ
                     PGMHNFARCLLRDDNPERDAGKAFANFLAAAEQEFPPSMGEVGHRYELGIGVATDSAK
                     ALAWYAKGAEWGDSFSCQRYGQLLFDGTGVKQDRARGLAMLILGGDPIAIKAAREQAD
                     EQLRKQATELYKQMRDDLEARENGG"
     gene            588..998
                     /locus_tag="PKO06_10280"
     CDS             588..998
                     /locus_tag="PKO06_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNV70076.1"
                     /translation="MAFDELLRQARETLRPRRLSAFAEAGGVAAALVSADGRIFTGVC
                     VDTTSSMGFCAEHAAAAALITAGESQVLKMVAVNWDGRILPPCGRCREFISQLHDQNR
                     STEVLVAEGKVVKLADLLPCDWKQVARSGVADGA"
     gene            1064..1849
                     /locus_tag="PKO06_10285"
     CDS             1064..1849
                     /locus_tag="PKO06_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV70077.1"
                     /translation="MSDFYFIHDRKAIAEYLKQHTDLHIYEIGDLDDFFWPHTMWFAD
                     AATAPFSAMCMLYAAQSQPSLLALGAAGPLTHLLRSITRVLPTWFYTHITPGAELAFA
                     EGYQLKPHGDHLKMSLRVPAMVAVVEETRAVRLGPENLAEVLNLYKVAYPGNWFDPRM
                     LETREYFGIREGGQLVSIAGVHVYSATQRVAALGNITTHPAHRKRGLAKIVTRKLCSS
                     LLEKVDYIGLNVAADNQAAIGVYRKLGFEVVAPYREYAVAKVI"
     gene            complement(1887..>3324)
                     /locus_tag="PKO06_10290"
     CDS             complement(1887..>3324)
                     /locus_tag="PKO06_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HNV70078.1"
                     /translation="LKDSDDVYARLIRLLLKQGKTREALERLEESHGKDMLDLLGDTL
                     NAPSRHWSQEIWDKVRRFRKELYELDVRDQGSNSPGDKRSATTRELREQVRADRQRAR
                     AGLEKALQEARASSAASSGSETGKINQSLLEGKGHACAGLITHPSVMTVIFQTRRDPC
                     WAFVLGGGKSEAVPLTISSHKIRLAAAKFRRAVVENEDDWVDKSHALSQIIWQPLAAF
                     VKDKPQVLIIPDGGLWYVPFAALSDLDGKPLITSRRLGICSTVNLAARLLERENQTSK
                     PKKALPGKILILADPDGSLPHARTEGAEIASLAGASGWQAETKVGSEASEIWLKRSVA
                     GVERGSLHFATHGILDSASPLFSYLLSTPGDGEDGQLQVLEIANELNLRPFSPAVLSA
                     CNTAMGTVSGGNEMICLQQAFHDAGVPEILASLWEVSDEATARLMKAFYTRHFGGTPV
                     GEALAEAIRPMTTEPPSRWAAFQLYGVP"
BASE COUNT          655 a          935 c         1032 g          702 t
ORIGIN      
        1 gcttgatatt tgccggctat ccgaaggcgc agaataacct tggcttgatc tatctcgatg
       61 gccgccccgg ggttccggcg aatatcaagc tcgctcacaa gtatctggga atggcggcag
      121 agcaaaagta ccagccgggc atgcacaact tcgcccggtg tctgctgcga gatgataacc
      181 cggagcgcga cgccggcaag gcgtttgcca acttcctggc ggccgcggag caggagtttc
      241 cgcccagtat gggtgaagtt gggcaccgat atgagctggg aatcggtgtg gccaccgatt
      301 cggcgaaagc cctggcctgg tatgcgaagg gcgctgagtg gggtgacagc ttctcgtgcc
      361 agcgctacgg tcagttgctg tttgatggaa ccggggtaaa acaggatcga gcccggggat
      421 tggcgatgct gattctcggt ggcgacccta ttgccatcaa agcagctcgt gaacaggcag
      481 atgaacagct gcgcaagcag gcgaccgagt tgtacaaaca gatgcgagat gatctcgaag
      541 cgcgcgaaaa tggcggttga gttgaaatga cgtcacggcg tatgacgatc gcatttgacg
      601 aactgctgcg gcaggctcgt gagacgcttc gcccacgccg tctttcggct tttgccgagg
      661 cggggggagt cgcagccgcg ctggtatccg cagacggaag gatctttacc ggagtctgcg
      721 tcgatacgac aagttccatg gggttctgcg ctgagcatgc ggcagccgcg gctcttatca
      781 cggcagggga aagtcaggtg ctgaaaatgg tggcggtgaa ctgggacgga cgcatactgc
      841 cgccctgcgg tcgctgtcgt gagttcatca gtcagttgca cgaccagaac cggtcgactg
      901 aagttctggt ggctgaaggg aaggttgtga agttagctga cctgcttccc tgtgattgga
      961 aacaggtggc gcggagtggg gtcgctgacg gagcgtagct cactggcacg acagacgaag
     1021 ttcaagctgc gcgtcgtggc ttgaagaaag agagataggg cgaatgtctg atttctattt
     1081 tattcatgat cggaaagcca ttgcggagta tctgaagcag cacactgatc tacacatcta
     1141 cgagatcgga gatctcgacg atttcttctg gccacacacg atgtggtttg ccgacgccgc
     1201 caccgctccg ttttcggcca tgtgcatgct ctatgccgcg cagtcgcagc cttcgctgct
     1261 ggctctcggg gctgctggtc cgttgacgca tctgctgcgc agcattaccc gcgtgctgcc
     1321 gacctggttt tacacgcaca tcactcccgg tgccgaactc gcgttcgccg agggatatca
     1381 gctcaaacca cacggggatc atctcaagat gagtttacgc gttccggcca tggtcgcagt
     1441 cgttgaggaa acgcgtgccg ttcggcttgg ccctgagaat cttgccgagg ttctcaactt
     1501 gtacaaagtg gcatatcctg ggaactggtt tgatccgcgc atgcttgaaa cacgcgaata
     1561 ctttggtatt cgcgaaggag gacagctggt cagcattgcg ggggtccatg tgtattcggc
     1621 gacccaacgg gtggccgcgc tggggaacat cacgactcac cccgctcatc gcaagcgtgg
     1681 tctcgccaag atcgtgaccc gcaaactgtg cagttccctg ctcgaaaagg tcgattatat
     1741 cggcctcaat gtcgccgccg acaatcaggc agccatcggc gtgtatcgca agctgggctt
     1801 tgaagtcgtc gcgccctatc gcgaatacgc ggttgccaag gtgatctgaa cccggacggg
     1861 tgtctcccct cgctggggag tcatcgtcac ggcactccgt acagctggaa ggccgcccag
     1921 cgcgatgggg gttcggtggt catcggccga atggcttcgg cgagcgcttc gccaacggga
     1981 gtgccgccga agtgtctggt atagaacgcc ttcatcagtc gtgccgtcgc ctcgtccgag
     2041 acttcccaca gcgatgcaag aatctcggga actccggcat cgtgaaacgc ctgttgcagg
     2101 cagatcattt cgttgcctcc cgaaaccgtt cccatggccg tgttacaggc agacagtacc
     2161 gccgggctga acgggcgcag gttcaactcg ttggcaatct cgagcacctg cagctgtcca
     2221 tcttcgccgt cgccaggggt tgagagcagg taggaaaaca gcgggctggc ggaatcgaga
     2281 atgccatgag tggcgaaatg cagagagccg cgttcgacac cggctaccga acgtttcagc
     2341 cagatctcgc tggcttcgct gccgaccttg gtctcagcct gccacccgct cgctccggca
     2401 aggctggcga tctcggctcc ctcggtgcgc gcatggggta acgaaccgtc cgggtcagcg
     2461 agaatcagaa tcttcccggg gagcgccttt ttcggtttgc ttgtctggtt ttcgcgctcg
     2521 agaagacgtg cggcgagatt gaccgtcgag cagatgccga gacgtcggct cgtgatcagc
     2581 ggcttcccgt cgagatcgct gagcgcggca aacggcacat accagagacc accgtcagga
     2641 atgatcaaaa cctgcggctt gtctttgaca aacgccgcaa gaggttgcca gatgatttga
     2701 gagagagcat ggctcttgtc cacccagtcg tcttcattct cgacgacggc gcgccgaaac
     2761 ttcgccgcgg caagtctgat cttatgactg gaaatcgtca gaggaactgc ttctgacttg
     2821 ccaccaccca ggacaaaagc ccagcatggg tcacggcgtg tctgaaagat cacggtcatc
     2881 accgagggat gggtgatgag cccggcgcag gcgtgtccct ttccttcgag caggctctga
     2941 ttgatctttc cggtctccga gcctgatgaa gccgccgagg aggctcgcgc ttcctgcaac
     3001 gccttctcaa gcccggcgcg agcccgctgc cggtcggccc gcacctgctc tcgaagttcg
     3061 cgggtcgtgg cgctgcgttt gtcacctggg ctgtttgagc cttggtcgcg aacatccagc
     3121 tcgtacagtt ccttgcgaaa ccgacgaact ttgtcccaga tctcctggct ccagtggcga
     3181 gagggagcat tgagggtgtc tccaaggaga tcgagcatgt cttttccatg tgattcttca
     3241 agacgttcga gcgcttcacg cgtcttgccc tgcttgagca gaagacggat caggcgggcg
     3301 tagacatcgt cgctgtcctt gaga
//
LOCUS       DAORHD010000451         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32524_length_3314_cov_2.900583, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000451 DAORHD010000000
VERSION     DAORHD010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32524_length_3314_cov_2.900583"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKO06_10295"
     CDS             <1..212
                     /locus_tag="PKO06_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70079.1"
                     /translation="LVSDTTVTDEYLCLTQNFKITPEELRKLIIYGFESTFLPYEEKE
                     EILREVEPILDRELLGKAWDKGREK"
     gene            261..1280
                     /locus_tag="PKO06_10300"
     CDS             261..1280
                     /locus_tag="PKO06_10300"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV70080.1"
                     /translation="MIRSVFCPKCRLRFEVIESPRLDSARCPHCAGQLDLGQHSHTVE
                     KLGHYQIIEMIGQGGMGQVFKARNPDGKLVAVKLMSIDLMSSAELTERFEREMDILAS
                     LQHENIVQLLDRGVTSAYKYFVMELVEGVTLRSLINTGSLTEAKIASIAVESLKALGY
                     AHAKGIIHRDIKPENILFDRHGKVKITDFGLARRLSTASGDSQGLTATNAFMGTENYM
                     SPEQKINPKAVTHKSDIYSFGVLLYEMLTGGHLPMGLFQPPSSFKTLDDFWDRLCFRM
                     LDINPDLRPENGVALAQEIEAYRSLSGINRRPFQRCQQANQPVGGETRAETRCRRSQR
                     RHVCG"
     gene            1222..1935
                     /locus_tag="PKO06_10305"
     CDS             1222..1935
                     /locus_tag="PKO06_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70081.1"
                     /translation="MKPEPKPAAAEVSEDTFAAENRRIQEQLKRLLDEGYRKTQAGGF
                     KDALKIWENALLLTSDAEEQKFIREWMDHCKYQLQEQNAPTNVAFLCPSCRKKFDKSA
                     SDPIPPEFTCPNCHAPLRYDAPRKQIALIKVKPPVAASEPDPGAFRRPVEKPCEGSLL
                     TQIVVVLAIVLLADFIYPSAINGVLQWAFEQGLSKVITVSPNVAAIYLRYALEAGLVI
                     LGALLLGQSILGLRDSSRK"
     gene            1939..2433
                     /locus_tag="PKO06_10310"
     CDS             1939..2433
                     /locus_tag="PKO06_10310"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70082.1"
                     /translation="MKTKLLLVLMLTSVFAVGWKTVGKDAGLTGIEVRDREQYLHRLK
                     FLRLKRPNEVQHTVQLGHLYYSLEMEDEAIKEYRRALKQDPENLDVRWFLSHVLSSKG
                     YQDEAFQLAREMIDRTPDDPEVYYWAGEVLEKLDQHEVAKEYFARVDEILLERKQLNS
                     KPTP"
     gene            complement(2499..3167)
                     /locus_tag="PKO06_10315"
     CDS             complement(2499..3167)
                     /locus_tag="PKO06_10315"
                     /inference="COORDINATES: protein motif:HMM:NF015945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HNV70083.1"
                     /translation="MGIFKRISMIIRSNINALIDKAEEPEKMLEQIIADMIENMREIK
                     LQVARSMRDEKMLERKVEENEKLAQEYTTKAELALEKGDDTLAREALKRRKSYQSIGE
                     SLKKELEEQHKVVEMLQTSFKALEVKIEEAKNKRQILLSRQKRAETQVDLSTTLDGVS
                     EQADLLESFERMADKVSRSEAMASALVDMEKGSVEEKFSKLEEEKSVEDELKLLKDRM
                     KSKK"
BASE COUNT          751 a          850 c          913 g          800 t
ORIGIN      
        1 gcctggtctc agacacgacc gtcactgatg agtatctttg cctgacccag aacttcaaaa
       61 ttacgcccga ggaactgcgc aaactgatca tctatggctt tgaaagcacg ttcttgccat
      121 atgaagagaa ggaggagatt ctgcgggagg ttgaacctat cctcgatcgg gaactgctgg
      181 ggaaggcatg ggacaaaggc cgcgagaaat gatgcatttc gatcgttcac ggcctatcga
      241 ggtgtgccga tagaagccgt atgattcgga gtgttttttg cccgaagtgt cggcttcgct
      301 ttgaagtcat tgagtcgccg cggcttgaca gcgcccggtg tccgcattgt gccggtcaac
      361 ttgatctcgg ccagcattcg cacacagttg aaaagttggg ccactatcag atcatcgaaa
      421 tgattggcca ggggggcatg ggtcaggtat tcaaggcgcg aaacccagac ggcaagctgg
      481 ttgccgtgaa gctgatgtcg atcgacctga tgagcagcgc cgaactgacc gagcgatttg
      541 aacgagaaat ggacattctc gcgtcgcttc agcacgagaa catcgtgcaa ttgctcgatc
      601 gcggtgtcac cagtgcctat aaatactttg tcatggaact ggtcgagggc gtaacgctca
      661 gaagcctgat caacaccgga tctctcacgg aggcgaaaat tgcctcgatt gcggtcgaat
      721 cattgaaagc gcttggttat gcgcatgcca agggcattat tcatcgcgat atcaaaccag
      781 agaacattct gttcgaccgg catgggaagg tgaagataac ggacttcggt cttgcccgtc
      841 gtttatcaac ggcaagtggt gattcccagg ggttgacggc gaccaacgcc ttcatgggca
      901 ccgaaaacta catgtcgccc gagcagaaaa tcaaccccaa agccgtaacg cataaaagcg
      961 atatttattc cttcggtgtt cttctgtatg aaatgctgac tggtggccat ctgccgatgg
     1021 ggttgttcca gcctccgtcg agcttcaaaa cgctggatga cttctgggat cggctctgct
     1081 tccgcatgct ggatatcaac cccgatcttc gtccggaaaa tggtgttgct ctggctcagg
     1141 aaatcgaagc ctatcgaagc ctatctggca tcaatcgcag accgttccag cgctgccagc
     1201 aagccaacca gccagtcggt ggtgaaaccc gagccgaaac ccgctgccgc agaagtcagc
     1261 gaagacacgt ttgcggctga aaaccgacgc attcaggaac agttgaaacg gttgctcgac
     1321 gaaggctatc ggaagaccca ggcgggggga ttcaaggacg cgttgaagat ctgggaaaac
     1381 gcattgttgc tcacctctga tgccgaagaa cagaagttta ttcgcgagtg gatggatcat
     1441 tgtaagtacc agttgcagga acagaatgca ccgaccaacg ttgcatttct gtgcccctca
     1501 tgtcgaaaaa agttcgacaa gtcggcgtct gatcccatcc cgccggagtt cacctgtccc
     1561 aattgtcatg cgcctctgcg ctatgatgcg ccgcggaagc agatcgcact gatcaaggtg
     1621 aaacccccag ttgccgcatc tgaacctgat cccggagctt ttcgtcgacc ggtggaaaaa
     1681 ccctgtgaag gcagcctgct gacgcagatc gtcgtggtgc ttgccatcgt tctgcttgcc
     1741 gactttatct acccgtcggc gatcaacgga gtgcttcagt gggcgttcga acaaggactg
     1801 tcgaaagtca ttacggtttc cccgaacgtg gcagccatct atctgcggta tgcgttagag
     1861 gcgggcctgg tcattctcgg ggccctgtta cttgggcagt ctattcttgg gttgcgcgat
     1921 tcatcgagga agtaacctat gaaaacgaaa ttgttactgg tactgatgtt gacctctgtt
     1981 tttgccgtcg gttggaagac ggtgggcaaa gatgccggtc tcaccggtat cgaggttcgc
     2041 gatcgcgaac agtatcttca tcgcctgaag tttctgcgct tgaagcgacc aaacgaggtg
     2101 cagcacaccg tccagctggg acatctctat tacagcctcg agatggagga cgaagcgatc
     2161 aaggagtatc gacgcgccct gaagcaggac ccggaaaacc tggatgtgcg ctggtttctc
     2221 agtcacgtgc tttcgagtaa ggggtatcag gacgaagcat ttcagctcgc gcgcgagatg
     2281 atcgatcgaa cccctgatga tcccgaagtc tactactggg ctggcgaggt tctcgaaaag
     2341 ctcgatcaac acgaggtcgc gaaagagtac ttcgctcggg tcgatgagat tctgctggaa
     2401 cgaaaacagc tgaattcaaa accgaccccc tgacagaaga gcatcgaaaa aacatgaaag
     2461 ccccgccaat cggcggggct ttttcaacgc gtggtcgttt acttcttcga tttcatgcgg
     2521 tctttcaaca gcttgagttc atcctcgacc gatttctctt cctcgagctt gctgaacttc
     2581 tcttcgacac tgcctttttc catgtcgacg agagcggaag ccatggcttc tgagcgcgaa
     2641 accttgtcag ccatgcgttc aaatgactca agcagatcgg cttgttcgct gacgccgtcg
     2701 agcgtggtcg agaggtcgac ctgggtttcg gcacgtttct gacgtgaaag cagaatctga
     2761 cgcttgttct tcgcttcttc gatcttgact tcgagggcct tgaagctggt ctgcagcatt
     2821 tcgacgactt tatgctgttc ctcgagttcc tttttcaggc tttcgccgat cgactgatag
     2881 gacttgcggc gcttgagcgc ttcgcgggca agggtgtcat ctcccttttc gagtgccagt
     2941 tccgctttgg tggtatattc ctgcgccagt ttttcgttct cttcgacctt gcgctcgagc
     3001 atcttctcgt cacgcatcga gcgtgcgacc tgcagtttga tttcgcgcat gttctcgatc
     3061 atgtcggcga tgatctgttc gagcattttt tccggttcct cggctttgtc aatcaaagcg
     3121 ttgatgttcg agcggatgat catggaaatg cgtttgaaaa tacccatggc tggacctcct
     3181 gaagattcgt tgtgctgatc atgaacttag caagcaaaat gccagatcgt gcaaaggctc
     3241 atcggaacgg gataggcctg aatgaaccca gaaagggaaa cgggaccgat gtgtcagatt
     3301 tgtcgcgtgg tcgg
//
LOCUS       DAORHD010000452         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32600_length_3309_cov_2.794714, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000452 DAORHD010000000
VERSION     DAORHD010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32600_length_3309_cov_2.794714"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKO06_10320"
     CDS             <1..364
                     /locus_tag="PKO06_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002691671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV70084.1"
                     /translation="SSKASIHRFVSETFHYDLDRTPDQIRPTYRFDVTCSGSVPEAIV
                     AFLHSNDYEHAVRLAVSLGGDSDTIACMTGSIAEAFYNGVPETIREQVFDRLDAHLKS
                     MVRKFIERFPLQRTSQNE"
     gene            complement(449..1234)
                     /locus_tag="PKO06_10325"
     CDS             complement(449..1234)
                     /locus_tag="PKO06_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV70085.1"
                     /translation="MSLPKRQYLISAYAEDLDWVTQLNEKTRVGVEIKDFSLPSSQGN
                     RERSFELWNLWRDSAPDLPVIVHAPFLDLVPGSPEPEVAELALKQHEEVMNLAEVLRA
                     ESVLFHSGFNPMIKSPQYQERWLASTSAYFNSLMKKHPTLKLLIENMWEPDPLPLIAL
                     IDSINSSQFRLCLDVGHVQVYSREAPDSWIRQLGLRLGLVHLNDNQGLWDQELALGTG
                     MVPLDHVFQAMNNLGYFGRVTIEMHGNEPILRSMEYLMKNGLA"
     gene            complement(1432..1749)
                     /locus_tag="PKO06_10330"
     CDS             complement(1432..1749)
                     /locus_tag="PKO06_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNV70086.1"
                     /translation="MGKKLYVGNMSYRTTNEELNEMFSQYGQVQSAEVIMDRATNRSK
                     GFGFVEMSNDQEAQAAISALNGKEVGGRQLTVNEARPKEPRQGGMGGGRGGFGGGRRG
                     DHF"
     gene            complement(2039..3124)
                     /locus_tag="PKO06_10335"
     CDS             complement(2039..3124)
                     /locus_tag="PKO06_10335"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNV70087.1"
                     /translation="MGTVIVPLEPTTFTLGDRNPVGGFYLMRSGDPAVYLVTSGADLA
                     FRKGANDLRARNIFHEEFAEIASLTLDSASGTMKLARNASGEWQILEPKPMKADSGEV
                     SALVFGIRDLRAHRFVQDNAAEADYARYGLDKPSLKAHLYDRSGKVYELHLGKEEGGE
                     VYVKRSDDASIYVFLKSALAILEKDVNQLRTKELVQVPRENIDRIKLVVATATVEVQR
                     VDKEWKTKDRVVPGNLVYALIDAVNALRVRRFHLWKERETFGLARLEGCEQVTLKHDG
                     AETKLLFGKTEGEEIYGYVEGREEVFRLPGSVRKAIMDLATEATKTPEPGTASGSVTP
                     PAVPAASASVAPAPVAPVTPPANPGAP"
     gene            3111..>3309
                     /locus_tag="PKO06_10340"
     CDS             3111..>3309
                     /locus_tag="PKO06_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70088.1"
                     /translation="MTVPITRPTTFTLVVLGANGQTLTRTASVSPVAVAPVAQISATL
                     HSPGLALVTWSTTNAVSASING"
BASE COUNT          674 a         1040 c          870 g          725 t
ORIGIN      
        1 ctcctccaaa gcctcaattc atcgtttcgt ctccgagacc ttccattacg atctcgaccg
       61 cacccccgac cagattcgcc cgacctaccg ttttgacgtg acctgcagcg gatcagttcc
      121 agaagcgatc gtcgcctttt tacactcgaa cgactacgag catgcggtgc gtctcgccgt
      181 ttcactcgga ggcgacagcg acacgatcgc ctgcatgacc ggcagcatcg cagaggcttt
      241 ttacaacggt gtccccgaga cgattcgcga acaggtcttc gatcgactcg atgctcatct
      301 gaagagcatg gtgcgcaaat tcatagaacg ctttccgctt cagcgtacaa gtcagaatga
      361 gtgaaaacga tcttctgaag ctcgtgtcaa aacagcaata atcgcaacac cttctggccc
      421 atgccgggcc ggaacagcgc ggtgcggact acgccagacc gttcttcatc aggtattcca
      481 tgctgcgcag aatcggctcg ttgccgtgca tttcgatggt cacccggcca aaatacccga
      541 ggttgttcat ggcctgaaac acatgatcga gtggcaccat gccggtaccc agcgccagtt
      601 cctgatccca gaggccctgg ttatcgttca gatgtaccag cccgagtctc agccctagtt
      661 gtctgatcca ggaatcagga gcctcacgcg agtacacctg cacatgaccg acatccagac
      721 acagacgaaa ctgggaagag ttgatcgaat cgatcaacgc gatcaggggc agggggtcgg
      781 gttcccacat gttctcgatc aacagcttca gcgtcgggtg cttcttcatc aagctgttaa
      841 agtatgccga cgtcgaagcc agccagcgtt cctgatactg cggggacttg atcatcggat
      901 tgaagccgga atgaaacagc accgactcgg cgcgcaacac ttccgccagg ttcatcactt
      961 cttcgtgctg cttgagggcc agttcggcga cttcgggctc cgggcttccg gggaccagat
     1021 cgagaaacgg cgcgtgaaca atgaccggaa gatccggtgc gctgtcacgc cagagattcc
     1081 agagctcaaa cgatcgctcc cggttgcctt ggctcgaagg cagagaaaag tccttgatct
     1141 caaccccgac gcgggttttc tcattcaact gggtaaccca gtcgagatct tccgcatagg
     1201 cagaaatcag atactgtcgc ttgggaagtg acatggatgt tcagtcctca ggtgtgattg
     1261 tcgactctgg cactatcgtt atcggatagt ttcagagcat ctcctacttc accagtctgc
     1321 gtcaagctct ttttcactcg ctcgtcagaa ttcgctccga gtgctgagag ccaaaaagcg
     1381 cccataaaaa aacccagcct tgcggctggg ttttgtgaag cttctaacgg attagaagtg
     1441 atcgccccgg cgtccaccgc cgaaaccacc acgaccgccg cccatgccac cctggcgggg
     1501 ttctttcggg cgggcttcgt tcacggtcag ctggcgaccg ccaacttcct tgccattcag
     1561 agcgctgatc gcggcctgag cttcctggtc gttgctcatt tcaacgaacc cgaaaccctt
     1621 gctgcgattg gtcgcgcgat ccatgatgac ttcggcactc tggacctggc cgtactggga
     1681 gaacatttcg ttcagttctt cgttggtggt ccgatagctc atgttgccga catacaattt
     1741 cttacccata acttcttttc gctccttcgc gatacactga aaatttcgac cctgacttat
     1801 cccgggcccc ggaaggccgc gcctcggcct aaggggtgga tcgcgaatga gaggcagaca
     1861 gattcactta ccgactcgct cactcaccaa ccgaggatta tcttatcacg aaccttgtcc
     1921 agatgcaagc aatttcgcgc agtcttccga aatcccgcat ctgatcgatg tctccagaaa
     1981 tacgctcggg ggtgtttctg cacccccgag cgatgttttc ccttgagccg aattccgttt
     2041 acggagcgcc agggtttgcc ggtggggtca ccggagccac gggagcggga gcgacagacg
     2101 cagacgcggc tggcaccgct gggggcgtca ccgaccctga tgctgtcccc ggttcgggtg
     2161 ttttggtggc ctcggtggcg agatccatga tcgccttgcg tacactgccc ggcaatcgga
     2221 agacttcttc gcggccttcc acatacccat aaatctcttc accctcggtc ttcccaaaga
     2281 gcagcttcgt ttcggcacca tcatgtttca gagtcacctg ctcgcagcct tcgaggcgcg
     2341 ccaggccaaa ggtttcacgc tctttccaga gatggaaacg gcgcacccgc aacgcgttga
     2401 ccgcatcgat cagcgcgtac accaggtttc cgggaacgac gcgatccttc gttttccact
     2461 ccttgtcgac gcgctgcacc tcgacggtcg cggtggccac aaccagcttg atccggtcaa
     2521 tgttctcgcg cggcacctgg accaattcct tggttcgcaa ctgattcacg tctttttcga
     2581 gaatggccag cgccgatttc aaaaacacgt agatgcttgc atcatcgctg cgcttgacgt
     2641 agacctcgcc cccttcctcc ttgcccagat gcagttcata gacctttccg ctgcgatcgt
     2701 acaggtgcgc cttcagactc ggcttatcga gcccataccg ggcatagtct gcttcggcgg
     2761 cattgtcctg cacaaagcga tgggcacgca gatcgcgaat accgaatacc aatgccgata
     2821 cttccccgct atccgccttc atgggcttgg gttcgagaat ctgccattcg cccgaagcgt
     2881 ttctggccag cttcatcgtt cccgaagcgc tgtcaagcgt gagcgacgcg atctcggcga
     2941 actcctcgtg gaaaatgttg cgggcccgca aatcattcgc cccttttcgg aacgccagat
     3001 cagcgccgct ggtgacgaga tagaccgccg gatcacccga acgcatcagg tagaagccgc
     3061 ccacggggtt gcgatccccg agcgtgaacg tggtcggctc gagcggcacg atgaccgtgc
     3121 ccatcacccg gccgaccacg ttcacgctcg tggtgctggg cgccaacggc caaaccctca
     3181 cgcggacggc cagtgtgtcg cccgtggccg tcgccccggt cgcgcagatc tccgcgacct
     3241 tgcatagtcc cggtctcgcg ctcgtcacct ggtccaccac caatgcggtg agtgcgtcga
     3301 tcaacggcc
//
LOCUS       DAORHD010000453         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32656_length_3304_cov_3.928901, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000453 DAORHD010000000
VERSION     DAORHD010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32656_length_3304_cov_3.928901"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKO06_10345"
     CDS             <1..786
                     /locus_tag="PKO06_10345"
                     /inference="COORDINATES: protein motif:HMM:NF019404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HNV70089.1"
                     /translation="GVTISSTRSYAHNRIWYPGPIDSTRFQNSTLYANPPAGNFTLHS
                     WSQKAPEVSLQAFRLRSELVEAISVEEPLRLPEVPPVFSTGPYKSSGAKLFENGVYLG
                     SSPSNPQIGDRRILFSVVKPPAVSIVARQTGKLFMPYQTNKGGEIEIIDRGTNTIDEL
                     FARAHQQTSFAAWVQRLFVVPWLAIGLYLLFLSETPLTLLFPGFSSLAQYGSIGRIAP
                     ICLSTAAIIVVTVWFAVQSPVIGAIVLTAVTAAALFRTRGEDA"
     gene            783..2858
                     /locus_tag="PKO06_10350"
     CDS             783..2858
                     /locus_tag="PKO06_10350"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HNV70090.1"
                     /translation="MIFPQGHTARGFALFALLVLVFCLVPEQLSFGRAGGGHSYSRSS
                     SSSSRSSSSRSSGSSRKSDTITTSSHSFPTGRSGSTSDGQPAYPAASGRYHLKARLPA
                     TQFFQSLFLSLFFAAIALFLINSRLPRRTIPPYTPVVNAALDTNVQGDRQRKAALERI
                     KQRDPQFSESALMKRISGAFCGIQSAWSKQDLTPVQAFLSDGVFERFSLQINDLTQDR
                     IRDVMKGLTILDARVVKVDTETHFDSLHILIRASAVNYREHLDTGAFIDGATDPEEFS
                     EVWSFLRRPGAKTLQKPGLIEGACPNCSAPIQMSRAATCPACQAFLRSGEHDWVLSEI
                     TQTCEWSGRAAAEILGLKAMQAADDGFNLQHLEDRVSVIFWRLMGCYRTGRTDPLLKY
                     ATSQFLADLTKRLAPDTYGKRTSYATPAVGSVDVVLVSHQEPMDQVVVEVRWSGKPVL
                     LDRQGKVLPDSMRPVNPHDLFVLKRQHSVKTNTRLSLSTATCPGCGAPETSATVNVCS
                     YCNTAYNDGSREWVLDQLLKKSDQAYHVLRRSVIESGKSKTGTQAASTPPAVPTPGPI
                     RSPADALRWMASLMCTDGTISPAEHEALKEYARVQHVPSRVLDGIVKDAQAGAALPDL
                     PNRPLENRALLQLLVVLALRDGTVSPEELEMLTAVGKRAGISKAELQRMIAVEQQKQA
                     TPHSPPDSV"
     gene            complement(2873..3199)
                     /locus_tag="PKO06_10355"
     CDS             complement(2873..3199)
                     /locus_tag="PKO06_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009594914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNV70091.1"
                     /translation="MASDQSYVDYVLEQLEGLEGVSYKKMFGEYGIYCEGKLVVMVCD
                     NQLFVKPTAAGREYIGAVTEASPYPGAKPCFLIDGAADDREWLCRLIRLTTDGLPTPT
                     PRRRTG"
     gene            complement(3204..>3304)
                     /locus_tag="PKO06_10360"
     CDS             complement(3204..>3304)
                     /locus_tag="PKO06_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNV70092.1"
                     /translation="MNTKTGRQLAEQRHAFLEQYLEQFFAEWQGER"
BASE COUNT          661 a         1072 c          889 g          682 t
ORIGIN      
        1 ggagtcacca tcagcagcac ccgctcctat gcccacaatc gtatctggta tccgggcccc
       61 atcgactcga cacgctttca gaactccacc ctgtatgcaa acccgcccgc cggaaacttc
      121 acccttcact cctggagcca gaaagcacca gaagtctcgc tccaggcttt tcgcctgagg
      181 tccgaactgg tcgaagccat ttcggtcgaa gagccgctgc gactgcccga ggtgccgccg
      241 gtattctcga cggggccata caagtcgtcg ggggcgaagc tgttcgaaaa tggcgtgtat
      301 ctcggaagct ctccgtcgaa cccacagatc ggtgatcgac gcattctctt ctcggtggtc
      361 aaaccacccg cggtatccat cgtcgcccgt cagacaggca agctcttcat gccgtatcag
      421 accaataaag gcggcgagat cgagatcatc gatcgcggca ccaacacgat tgacgaactc
      481 ttcgctcggg cgcaccagca aaccagtttt gccgcctggg tacaacggtt gttcgtcgtt
      541 ccctggctgg ccatcggact ctatctgctc tttctgtctg aaacgccgct gacgctgttg
      601 tttcccggct tctcgtcact ggcgcaatac ggctccatcg ggcgcatcgc ccccatctgt
      661 ctcagcacag ccgcgatcat cgtggttacg gtctggtttg cagtacaatc gccagtcatc
      721 ggagctatcg tcttgacggc tgtcacggcc gccgcccttt tccgcacacg aggtgaagac
      781 gcatgatttt ccctcaaggt cacacggcac gtggattcgc gctgttcgcc ctgctcgttc
      841 tcgtcttctg ccttgttccc gagcaactct cattcggccg ggccggaggc gggcacagct
      901 actcgcggtc gagttcctcc agcagccgtt cgagttcgtc ccgcagttcg ggcagctcgc
      961 gcaagtcaga caccatcacc acctccagcc attcgtttcc caccggcaga tccggttcca
     1021 cgtcggatgg ccaacctgca tatccggcgg cgagcggccg ctatcacctg aaagcacgcc
     1081 tgccggcgac ccagttcttc cagtcgctgt ttctcagcct gttcttcgcc gccatcgcac
     1141 tgtttctgat caacagccga ctgccgcgac gcacgattcc cccctacacc ccggttgtga
     1201 atgccgcgct cgataccaat gttcagggag atcggcaacg aaaggccgcc ctggagcgca
     1261 tcaagcaacg cgacccgcag ttctcggaat ccgctttgat gaaacgcatc agcggcgcat
     1321 tctgcggcat tcagagcgca tggtcgaaac aggacctgac gccggtgcag gcgtttctgt
     1381 ccgacggcgt cttcgagcga ttctcgctgc agatcaatga cctgacacaa gaccgcattc
     1441 gagatgtcat gaaggggctg accattctgg acgcccgcgt cgtcaaggtc gataccgaga
     1501 cacactttga ttctctgcat attctcattc gggcttcggc cgtgaactat cgggaacatc
     1561 tggacaccgg agcgttcatc gacggcgcca ccgacccgga agaattttca gaggtctggt
     1621 catttctgcg gcggcccgga gccaaaaccc tgcagaaacc cggattgatc gaaggtgcct
     1681 gcccgaactg ctcggctccg attcaaatgt cacgtgccgc gacctgcccg gcatgtcaag
     1741 catttctgcg ctcgggtgag catgactggg tactgtctga aattacgcaa acttgtgagt
     1801 ggtctggtcg agccgcggca gaaattctcg ggctgaaagc catgcaggcc gcggacgacg
     1861 gttttaatct gcaacatctc gaagaccgcg tttccgtcat cttctggcgc ctgatgggct
     1921 gttatcgcac cggtcgcacc gatcctctgc tgaagtatgc caccagccag tttcttgccg
     1981 atctcaccaa acgactggcc cccgacacct atggcaagcg taccagttac gccacgcccg
     2041 ccgtcggatc agtagacgtc gtgctggttt cacatcagga acccatggat caggtcgtcg
     2101 tcgaggtccg gtggagcggg aaacccgtgt tgctcgacag gcaaggaaaa gtgctccccg
     2161 actccatgag accggtgaac ccgcacgacc tgttcgtgtt gaagcgacag cattcggtca
     2221 agaccaacac gcgtctctct ttgtcgactg caacctgccc gggctgcggt gcgcccgaaa
     2281 ccagcgcaac agtgaacgtc tgctcgtact gtaacactgc ctacaatgac ggttcgcgcg
     2341 agtgggtcct cgaccagctg ctcaagaaat ctgaccaggc gtatcatgtc ctgcgtcggt
     2401 ctgtcatcga gagcggcaag tcgaaaaccg gcacacaggc cgcgtctact ccgcccgccg
     2461 taccgacacc gggaccaatt cggtcgcccg ccgatgctct gcgctggatg gcctcactga
     2521 tgtgcaccga tggcaccatt tcgccagccg agcatgaagc gctgaaagaa tatgcccggg
     2581 tgcagcacgt gccaagccgg gtgctcgacg gtatcgtcaa ggatgctcaa gcaggcgcgg
     2641 cccttcccga tctgccgaac cgcccgctcg aaaaccgcgc actgctgcaa ttgctggtcg
     2701 tcttggcgct cagagacggt accgtttcgc cggaggaact cgagatgctc accgctgttg
     2761 gcaaacgcgc cggaatcagc aaagccgagc tgcagcgcat gattgccgtg gaacagcaga
     2821 aacaggcgac accgcacagc ccacccgatt cagtctgaga cgagacagcc cgtcagccgg
     2881 ttcgccttct cggcgtgggg gtcggcagac catcggtggt cagtcgtatc agtcggcaca
     2941 accactcccg gtcgtcagcg gcgccatcga tgagaaagca gggcttcgcc ccaggatacg
     3001 gtgacgcttc agtcaccgcg ccgatgtatt cgcgcccggc cgcggtcggt ttcacgaaca
     3061 actgattgtc gcacaccatg acaaccagct ttccctcgca atagatgcca tattcaccaa
     3121 acatcttctt gtacgataca ccttcaagac cttcgagctg ttcgagaaca tagtctacgt
     3181 agctttggtc cgaggccatg cgttcacctt tctccctgcc attcggcaaa gaattgctcg
     3241 agatactgtt cgagaaacgc gtgccgctgt tcggcaagct gtcgcccggt tttcgtattc
     3301 attc
//
LOCUS       DAORHD010000454         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32663_length_3304_cov_3.204063, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000454 DAORHD010000000
VERSION     DAORHD010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32663_length_3304_cov_3.204063"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKO06_10365"
     CDS             <1..665
                     /locus_tag="PKO06_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV70093.1"
                     /translation="PEYAANTNPELVEAVVKSCLEAGAKEVKIFDNTCNNAQRCYINS
                     GIQDKAEAAGAKVYHVQEDRFKSIKLTKGEILKEWPVYQDYFEVNKVINLPIAKHHGL
                     ATVTLGLKNLMGVMGGNRGELHSPFRKLIDVVADILPTLTIIDAYRMLVDHGPRGGDL
                     ADVKLTKTLIASPCTVAADVTALELFGHQIKDIPHIADAIDRGLAKVDLKNLKPLKLK
                     LG"
     gene            921..2372
                     /locus_tag="PKO06_10370"
     CDS             921..2372
                     /locus_tag="PKO06_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV70094.1"
                     /translation="MRFPFSPTPDPDLYLRCSPLGPLWALLTGSVPVGVFWGGLVVCF
                     FTLFFGRFFCGWVCPLGTCIDVTDAAVAPRSPSTAQPTMQHTRYVILLGGLVATFFSV
                     NVLWYFDPLSLFTRLTTVILFPCMTWLVSLVLKTLAIIPGVASIDWYWRRYVFIEGQA
                     SHLQTGALFLVCAGLFLLARSYRRGWCRFVCPAGAFLGLLSRLLPLGRSVTEECIHCD
                     RCSRECRMGAIPTGKVALTHPDHCIQCLECGVLCPVPGSAIRFGVRPRLHPPLDLGRR
                     ELMQGVLAGITATGLHRLDAEQPARHHPLLRPPGAVPEADFLQRCLRCLACVRVCHTN
                     GGCLQPADGSSRLLDRWTPVAVMRLGYCEHNCNLCGQVCPTGAILPLSLKDKQENAMG
                     LAVFDRNVCIPHARAEDCLVCEEHCPIGDKAIRFDQREVRAPDGSTRLVKLPYVVKDK
                     CIGCGICEAKCPLKGNPGIFVTPENADRPRHSI"
     gene            complement(2740..>3304)
                     /locus_tag="PKO06_10375"
     CDS             complement(2740..>3304)
                     /locus_tag="PKO06_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70095.1"
                     /translation="DMTGTNAYLTPCMRMILVAVVAISFVSLTAPARASEDGELWWEK
                     PESVSATCGARNAWGHELTSDRSLKRNVGGASMLTRPSRQIIPGKVSSVEFVMSTEDP
                     DVVGVLQVTRNDGEIFRFRVFTYTLILIRDAHGKLWAGRLKHLRPGWACEVAFDLPPD
                     EDDNIDFRPEIDPLIDAASVIADSFHQ"
BASE COUNT          646 a          992 c          884 g          782 t
ORIGIN      
        1 ccccggaata tgcagccaac accaatcccg aactggtcga agccgtcgtg aaatcatgtc
       61 tggaagccgg tgccaaggaa gtgaaaatct ttgacaatac gtgcaacaac gcccagaggt
      121 gctacatcaa cagcggcatt caagacaagg cggaagctgc gggcgcgaag gtgtatcacg
      181 tgcaggaaga ccgcttcaag tcgataaaac tgacgaaggg cgagattctc aaggaatggc
      241 cagtgtatca ggactatttc gaagtgaata aggtcatcaa cctgcccatc gcgaaacatc
      301 acgggctggc gacggtaact ctcgggttga aaaacctgat gggcgtcatg gggggaaacc
      361 ggggcgagtt acattcacca ttccgcaagc tcatcgacgt cgttgccgac attctcccga
      421 cactcaccat cattgatgcg tatcgcatgc tggtcgatca cggcccacgc ggcggagatc
      481 tcgccgatgt caaactgacc aagacgctga ttgccagtcc ttgtacggta gccgcagatg
      541 tgacagccct cgagttgttc ggtcatcaga tcaaggatat tcctcacatc gccgacgcca
      601 tcgatcgcgg cctcgcgaaa gttgatctga aaaatctgaa acccctgaag ttgaaactcg
      661 gctgaacccg tctggccgac gccggtgttg atctcgcaat agtacaagtg acattgcgcg
      721 catgttgagt tcttgaaagc ccaagcactc acgtattatt caatccgcga ggagtccgaa
      781 tgtcttcggt tacgcctgaa caagtgtcgg aatcgtctcc tgcccaacgg ccgcgaactt
      841 cctggctatt cgcagcctcg tcgctccgtc gtctcgttca ggcggcattt ctaattggta
      901 cgctggttct gttcgttcgc atgcgttttc cgttctcccc caccccggac ccggatctgt
      961 atcttcgttg cagtccactc ggaccattgt gggctttgtt aactggatca gtacccgttg
     1021 gtgtcttctg gggcggcctt gtggtgtgct ttttcaccct gttcttcggc cgttttttct
     1081 gcggttgggt ctgtccactc ggcacctgca ttgatgtcac tgatgcggcg gtggctcctc
     1141 gttcgccttc aacggcccag ccaaccatgc agcacactcg gtatgtgatt ctccttggcg
     1201 gacttgtcgc gacgtttttt tctgtcaatg ttctgtggta tttcgatccc ctctcgttgt
     1261 tcacccggtt aacgacggtg attctgttcc cgtgtatgac ctggctcgtt tcgctggttc
     1321 tgaaaactct tgccatcatt cccggagtag cctcgatcga ctggtactgg cggcgctacg
     1381 tgttcatcga gggtcaggct tcccatttgc agacgggggc cctctttctc gtatgcgccg
     1441 gtctgtttct tcttgcccgc tcgtatcgtc gggggtggtg tcgatttgtt tgccctgccg
     1501 gagcgtttct cggcttgctt tcccggcttt tgcccctcgg acgatcggtc accgaagaat
     1561 gtattcactg tgatcgctgt tcacgtgaat gtcgcatggg ggcgattccg accggcaagg
     1621 tggcactcac gcacccagac cattgcattc agtgcctcga atgcggcgtt ctctgccctg
     1681 ttcctggctc tgccattcgc tttggcgttc gcccccggtt acaccctccg cttgacctcg
     1741 gccgccgaga actcatgcag ggagtgctcg caggtatcac tgccacagga ttgcaccgtc
     1801 tcgacgccga acaaccagca aggcaccacc ctctcctgcg cccaccgggc gctgttcccg
     1861 aagcggattt cctgcagcgt tgtcttcggt gtctggcctg cgttcgcgtc tgccacacca
     1921 atggcggatg tcttcagcca gctgatggct cgagtcgcct gctcgaccgt tggacccctg
     1981 tcgccgtgat gcgcctgggc tattgtgagc ataactgcaa cctgtgcggt caggtctgcc
     2041 ctaccggcgc catccttccg ctttcgctga aagacaagca ggaaaacgcc atggggctcg
     2101 ctgttttcga tcgcaatgtg tgtattccac atgcccgagc cgaagactgt ctggtgtgcg
     2161 aggaacactg cccgattggt gacaaagcca ttcggttcga tcagcgagaa gtccgagctc
     2221 ctgacggttc tacacgactc gtgaagcttc cctacgtggt caaggataaa tgtatcggtt
     2281 gcggcatctg cgaagccaaa tgcccgctga aaggaaaccc cggcattttc gtcacgcccg
     2341 aaaacgccga ccggccaaga cattcaatct gaggcgctca gtcgtcgtga cgaggaacca
     2401 tttcttttca ttcgggaatt catgtctcga caactcactc gcgatgcaag tctgaaaagc
     2461 atttctcgtc tccaacgaaa tcggcatgag gttgttcccg gaaacggctg tcagcagaag
     2521 atttcgcgat ctggtcgggc gctttaacca ccgcgtcgca gccatcgccg ataacgtcac
     2581 atttctgatc tcgggacaac cactgcccgt caggcacaat cggcgcgccg ttcacctggt
     2641 ctcagcctgg acggaatcgt cgtcacggcc cgtaaacgag aaccgaagct gatgttcact
     2701 ttgtctgacg aatggtcttc ttcgcaagag cacaaggaac tactggtgaa atgagtccgc
     2761 gatgacgctg gcggcgtcga tcagcgggtc gatctccggt cgaaaatcta tgttgtcgtc
     2821 ttcatcgggc ggcagatcga aggcgacttc gcaggcccaa ccgggcctga ggtgtttcaa
     2881 ccgcccggcc cacagcttcc cgtgggcatc gcggatcaaa atcagcgtat aggtgaacac
     2941 ccgaaaccgg aagatctcgc cgtcgtttcg cgttacctgc aaaaccccga cgacatcagg
     3001 gtcttcagtc gacatgacga actccacgct cgacaccttt ccgggaatga tctggcggga
     3061 cggacgcgtc agcatactcg cgcccccgac gttccgcttc agggaacgat cgctcgtcag
     3121 ttcgtgaccc cacgcgtttc tcgcgccaca cgtcgcagac accgattccg gtttttccca
     3181 ccagagttcg ccgtcttcgc tcgcgcgcgc cggcgccgtc aacgacacga agctgatcgc
     3241 gacgaccgct accaggatca ttcgcataca tggtgtgaga tacgcgttgg ttcctgtcat
     3301 atct
//
LOCUS       DAORHD010000455         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32674_length_3303_cov_4.405172, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000455 DAORHD010000000
VERSION     DAORHD010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32674_length_3303_cov_4.405172"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKO06_10380"
     CDS             <1..216
                     /locus_tag="PKO06_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV70096.1"
                     /translation="ADAQLILGPVDEVQNFYQASGFSGHGLMLAPAVAMYMAKMVTTG
                     EVHPEIQGLRASRFKGMKEVHREKSVV"
     gene            complement(258..1038)
                     /locus_tag="PKO06_10385"
                     /pseudo
     CDS             complement(258..1038)
                     /locus_tag="PKO06_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003490437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(1247..3094)
                     /locus_tag="PKO06_10390"
     CDS             complement(1247..3094)
                     /locus_tag="PKO06_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70097.1"
                     /translation="MIRGSLMCSVFLSIVFSLVFLNPLMAAQEMVHDYPVRELTAANA
                     KSLLADLEAEHAQLLKKLQKEDPDKVKKSVPLWAYERIIAMTRTVQAGLKENAVDPGL
                     ALTFNQIQRVHYSVLADLGGGAPEPRIKRLWQMSTNVGFGPLKLNVPDVIIPSQALGR
                     RVAGKESLGLFDQKGSLVSPDQYATLSALDISRLQPRQDHFAILPIEPGNHYEAFLRD
                     QVERIKACSKKYEQFDLTYARRVLFFDELKEDATSPKITAKDRYGLKWKVKWGDEVHT
                     DVALTRIYIDLGAPYTDLKFYSGPGETILVLAPPSETDPAQPKTFAAMADQLLKSKFK
                     FHADRYLLPKPVLTDKTGTILGTGIVTTEMAEKESISPKYVGAYFVTFKECQLSLYNP
                     AIKRLGGASMNKLRATQDRVARSSLVFNAWVKNKDVKDDNTRAGFLFNPQTGDYDQYV
                     EFISDLGCSLGHLKPSGELNSFEHKLIMMMPQTINFFMRPLYIPESWKDCTWADARWM
                     SLRIARLTRADLERAFLDCGWPIFAQKVAVEKLISRRNDLVEAFHLEEDGIEKLPCDV
                     GFSFEVKTKNGVDYPVYKGKINENSEVVRDLEETVHPEGLANIISRKND"
BASE COUNT          689 a          858 c          986 g          770 t
ORIGIN      
        1 gctgacgctc agctgatcct gggcccggtt gatgaagtgc aaaacttcta tcaggccagc
       61 ggattttcag gccatggttt gatgctggca cctgccgtcg ccatgtatat ggcgaagatg
      121 gtcacgaccg gtgaagtgca ccccgagatt caggggttgc gcgccagtcg gttcaagggc
      181 atgaaggaag tgcatcgcga aaaatcggtc gtctgatacc gacgacgaca gtcgaggaaa
      241 accgcaacgc cggctcatca gggtcggcgt tgcgcctttg cgggaggggt ggcgcgcggg
      301 gttgtcgacc ggtggtgtgt ggctttcttc tgcgcactct gttcgaggag agacacttct
      361 tcgccggtca gtagccgcca ggcgcctttc gcgagcgttc cgagcggaat cggaccgatg
      421 gcgactcgga caagacgcag aacttcgata tgaaacgctt ccagaagacg gcgtatctga
      481 cggttcttgc cttcgtcgag cacgatctcg agccaggcat tttttttcac ctgaacgcag
      541 aaaagagacg tgcttggcat tcacatagcc tatttcagag acggtgacgc ctttcatgag
      601 ctgctcgaga agttccgcgt ctggtactcg gttgatctga acatgatagg ttttgtcgag
      661 gtgggaatcg ggcgaggtca gtcggtcaga ccactgggta tcgttggtaa agaacagcag
      721 cccttcgctg gctttgtcca gtctgccaac aggaaatact cgctcacctc gccagtctga
      781 aaaacaatcg tagacggtgt cgcgaccttt ctcgtcgcaa gcggtcgtga tgaagccgcg
      841 aggcttgttc aaagcgagat agactttggt cgcggcgaca accggcgtgc cgtcgacgga
      901 gatctggtcg tgatcaaggt tgactcggcg ctccgggtca cgacatgtct gaccattcac
      961 ctgtacgcgt ccgagagtga ccagatagtg agcctgtgac cgcgaggtat accccagttt
     1021 cgacagagcg cgttccagag ctgttgtgac ctggtttccc cggttcagtg gcacacgtgg
     1081 tcgcgcgtgg ctgcttctcg attcggtgtg ctgtcgcgat gatctgggag cgggtgaaga
     1141 gagacggttg gttcggttca tgcggttgct tctgacggct cagaaaagag agaaccgccg
     1201 gtttcgcaga ctgccggcgg ttctctggtt gagatctttc tgcgtcctag tcgttcttgc
     1261 gcgaaatgat gttcgccaag ccttcaggat gaactgtttc ttcgagatcg cgaacgactt
     1321 ctgagttttc gttgatcttt cctttgtaga ccggataatc aaccccgttc ttggttttca
     1381 cttcgaacga gaaaccgacg tcgcacggaa gtttctcgat accatcttct tcaagatgga
     1441 aagcctcgac cagatcatta cgccgcgaga tcagcttctc gactgcgact ttctgcgcga
     1501 agatcggcca gccgcaatca agaaatgccc gttcgagatc ggcacgggtg agtctggcaa
     1561 tgcgcaggga catccagcgg gcgtcagccc acgtgcaatc tttccaggat tccggaatgt
     1621 acagggggcg catgaagaag ttgatggtct ggggcatcat catgatgagc ttgtgttcga
     1681 acgaattcag ctcgcccgaa ggtttcaaat gtccgagtga gcagcccaga tcgctgatga
     1741 attcaacata ttggtcgtag tccccggtct gtggattgaa caggaacccg gcgcgggtgt
     1801 tgtcatcctt cacatccttg ttcttgaccc aggcgttgaa caccagagaa ctgcgggcga
     1861 cgcggtcctg cgtggcgcgc aacttgttca tcgaagcccc gccgagacgc ttgatggcgg
     1921 ggttgtacag tgacagctgg cattccttga aggtcacgaa gtatgcgcca acgtatttcg
     1981 gactgatcga ttctttctcg gccatttcgg tcgtgacaat gccagtgccg agaatagtac
     2041 cggttttgtc ggtgagaaca ggcttgggaa gcagatagcg atcagcgtga aatttaaatt
     2101 ttgatttcag cagctggtcg gccatggccg caaacgtttt cggctgtgcc ggatcagtct
     2161 cgctgggtgg tgcgagtacc agaatcgttt cgccggggcc ggagtaaaac ttgagatctg
     2221 tataaggcgc gccgagatca atgtagatgc gtgtcagggc cacatcggta tggacttcgt
     2281 caccccactt gaccttccat ttcaggccat aacgatcttt ggccgtaatc ttgggtgagg
     2341 tggcgtcttc tttcaattcg tcgaagaaca agacacgacg agcatacgtc aggtcgaact
     2401 gctcgtattt cttgctacag gctttgattc gctcgacctg gtcgcgcaga aacgcctcgt
     2461 agtgattgcc cggctcgatc ggcagaatgg caaagtggtc ctggcggggc tgtaaccgcg
     2521 agatgtcgag agcgctgagt gtggcatact ggtcggggga aaccagcgag cctttctgat
     2581 cgaatagacc gagagactct ttcccggcca ctcgtctgcc gagtgcctgc gatggaatga
     2641 tgacatcagg aacgttcagc ttgagtggac cgaaaccgac attggtgctc atctgccaga
     2701 gacgtttgat tcgtggctcg ggagcaccgc cgccgaggtc ggcgaggacg ctgtagtgaa
     2761 cccgctgaat ctgattgaag gtcagcgcaa gaccgggatc aacggcattc tctttcaacc
     2821 cagcctgtac tgttctcgtc atcgcgatga tgcgctcgta cgcccagagc ggcacacttt
     2881 tcttgacctt gtcggggtct tccttttgca gcttcttgag cagttgcgcg tgttcagctt
     2941 cgagatcggc aagcagagat tttgcgttgg cagctgtcag ttcgcgaacc ggatagtcat
     3001 ggaccatctc ctgagccgcc atcagcgggt tcagaaacac cagcgagaag acgatcgaca
     3061 aaaacactga gcacatcaga cttccacgga tcatgcaata cctccccact cgactgtacc
     3121 cacatggtat cagaggtcga ggcgtgggta aaggagccgt atccaggtgc cgtgttggaa
     3181 accgcggcga taccgttcag tctttcggtg gccagccgat ctcgcgagcg atgggttgat
     3241 caacccggcc ggccagaagg tgcaacgcaa acagttcctt gagtggctct gcggtggcag
     3301 ggc
//
LOCUS       DAORHD010000456         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32724_length_3300_cov_4.600308, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000456 DAORHD010000000
VERSION     DAORHD010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32724_length_3300_cov_4.600308"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKO06_10395"
     CDS             complement(<1..630)
                     /locus_tag="PKO06_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV70098.1"
                     /translation="MTHKPFYVTTPIYYANAELHIGHAFTTMAADIAKRYHLAMGREA
                     HFLTGSDEHGQKVEQAAKLRNITPQQHVDEIVEKFRTLWKKLEVPYDDFIRTTEPRHK
                     TSVQFVLQKLYDKGDIYADTYTGWYCTPCERFWTEKEVPEKLCPDCKRAVEEIQEKNY
                     FFRMSKYQQRLIEYIQAHPGFIRPEMRKNEVLGFLAQPLGDLCISRPKKR"
     gene            complement(635..2113)
                     /locus_tag="PKO06_10400"
     CDS             complement(635..2113)
                     /locus_tag="PKO06_10400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV70099.1"
                     /translation="MELFRNVSIGQRVILTMLLALALPIGIFVYVRWMDLATNFKVFL
                     FLGNIFLLLGLISAWLVARSITRPLDRLRRRVASFVEKRVAQPVNDPGEDEIAELGHE
                     ITALFGIWNNEVGRMLVKQKQRGEETLKVTAAQTQLERQLELSRSCLAVAQRLNTSFH
                     FQTNLQTILDEAIKTLNIQWGSVLLINRQTLELGVACVRGIEQSYVDDIIEDQYPAIK
                     LKPSEGLAGAVIKSGMPLIANKGHRDSRFKTFSEFKTREEKVASIMCAPIKGSDGTVL
                     GVVNFINRISPPIFRDEDIPYIQDLCTLVALVIERNNLYTQVFADETTGLIGFRIWKS
                     FMEEEAVRAARYAHAISVIALEVDHFRTHVSATNEDFGRQVLGELGQSLKKVLRDSDA
                     ATRVQERYYLLLPNTDAAGAVFLVGRVKELLEKQTFEFQGKSVSVTISAGIAAYPETN
                     PDARQLFKTSNLALEQATAAGSNRAVVFGRTPETAAQPHSHR"
     gene            2332..>3300
                     /locus_tag="PKO06_10405"
     CDS             2332..>3300
                     /locus_tag="PKO06_10405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70100.1"
                     /translation="MSDNNEFSFWSLVPLWLRLTIFFGLLIIAAPFLLIGHSKNGTIE
                     RSMKQGAEYLGKGNFIDAGKEYEKAGAQFGFLYDCYKLALNVTGGTYYKKNVCLMLRG
                     MARAGAIAERLGNGDTDVDQEIKDARADLLITADIPADLNQARTQAKDELDRIEKMQP
                     LVMLCKDGKYIQIMEHLDSMIKGGTVPFGEMVGMPTCYVLSECAVNLKTADAIETAKK
                     ICFLHSQKFKYPLFQKLAFKVTSLTAENVAVKPSAAKPTATAAKPAGDAKERFKLAMS
                     YASKKDFQKAMPLFESCYKDNPKNVDFVYFFALAQSKAGKPDEAQKL"
BASE COUNT          691 a          870 c         1031 g          708 t
ORIGIN      
        1 gcgcttcttg ggtcgtgaga tgcagagatc gcccagcggc tgcgcgagaa atccgagcac
       61 ttcgttcttg cgcatctctg gtctgatgaa gcccgggtgc gcctgaatgt actcgatcag
      121 tcgctgctga tacttcgaca tcctgaagaa gtagttcttt tcctgaatct cttcgaccgc
      181 acgcttgcag tctgggcaga gcttctcggg cacttccttt tccgtccaga agcgttcgca
      241 gggcgtgcag taccagccgg tatacgtgtc agcatagatg tcgcccttgt catagagctt
      301 ctgaagcacg aactgcaccg aggtcttgtg ccgtggttca gtggtgcgaa tgaagtcgtc
      361 ataggggact tcgagtttct tccagagcgt gcgaaacttt tcgacgatct cgtcgacatg
      421 ctgctgcggg gtgatgttgc gcagtttggc ggcctgctcg accttctggc cgtgttcgtc
      481 cgacccggtc agaaagtggg cttcgcgacc catggccagg tgataccgtt tggcgatgtc
      541 ggcagccatc gtcgtgaagg catgaccgat atgcagttcg gcgttggcgt agtaaatggg
      601 agtggtgacg tagaagggtt tgtgtgtcat gggttcacct gtgtgagtgg ggttgagcgg
      661 ctgtctcggg cgtacgaccg aagacgacgg cacggttgct gccggcggcg gtggcctgct
      721 cgagcgcgag attcgaagtt ttgaacaact ggcgggcatc ggggttggtt tcggggtagg
      781 cggcgatacc ggcggagatc gtgaccgaga cagacttgcc ctgaaactcg aaggtctgct
      841 tttcgagcag ttctttcacg cgtccgacca gaaagacggc accggccgca tcggtattgg
      901 gaagcagcag atagtaccgt tcctgcacac gtgtcgcggc gtccgagtca cgcaagactt
      961 tcttcagtga ctgtcccagt tcaccgagca cctgacgccc gaaatcttcg ttggtggcgc
     1021 tgacatgagt ccggaagtga tcgacctcaa gcgcgatgac cgagatcgcg tgggcgtagc
     1081 gggcggcccg cacggcttcc tcttccatga acgacttcca gatgcgaaac ccgatcaacc
     1141 cggtggtctc atcggcgaac acctgagtgt acaagttgtt gcgctcgatc accagcgcga
     1201 cgagcgtgca gagatcttga atgtagggaa tatcttcgtc gcggaaaatc ggcggcgaaa
     1261 tgcggttgat gaagttgacc acgccgagca ccgtgccgtc gctgcccttg atcggtgcgc
     1321 acatgatgct ggcgactttt tcctcgcggg tcttgaactc tgaaaaggtt ttgaagcgac
     1381 tgtcgcgatg ccctttgttg gcgatcaggg gcatgcctga cttgatgacc gcaccggcga
     1441 ggccctcgct gggtttcaac ttgatagccg gatactggtc ttcaatgatg tcgtcgacgt
     1501 atgactgctc gatgccgcgc acgcaggcga cgccgagttc gagggtttga cggttgatca
     1561 gcagtaccga gccccactga atgttgaggg tcttgatcgc ttcgtcgaga atggtctgta
     1621 gattggtctg aaagtgaaag ctggtgttca gccgctgggc cacggccaga cacgaccgcg
     1681 acaactcgag ctgccgttcg agctgggtct gggccgcggt cactttgagg gtttcttccc
     1741 cgcgctgttt ctgcttgacg agcatgcggc cgacttcgtt gttccagatc ccgaacaggg
     1801 cggtgatctc gtggccgagt tcggcgatct cgtcttcgcc ggggtcgttg accggctgag
     1861 cgacgcgctt ctcgacgaag ctcgccacac ggcgtcgcag gcgatcgagc ggtcgggtga
     1921 tcgaacgggc gaccagccag gctgagatga gccccagcag cagaaagatg ttgcccagaa
     1981 acaaaaagac cttgaagttg gtggccagat ccatccagcg aacatagacg aagatgccga
     2041 tgggaagagc caatgccaga agcatggtca aaatgacccg ctggccgata ctgacgttac
     2101 ggaagagttc cactgacgac ctctctctga gatgtggcct gaattctagg ccacgcccgg
     2161 tgaaaagtca aagaccatcc cacaggtctg gggactaggt ggtctcctgc ttgggttttc
     2221 ccgaggaata ctggatagat tcaaatgatc ctgttcagac cgggcaggga ttgacggtct
     2281 cgggtataat gaaggtagtg tgtagcatgt acgtgagaga gggggggagt catgtctgac
     2341 aacaacgaat tttccttctg gtcgctggta cctttgtggc tgcggctgac gatcttcttc
     2401 gggttgttga tcattgccgc gccgttcctg ttgatagggc attcgaagaa cggcaccatc
     2461 gagcggtcca tgaagcaggg agccgagtac ctcggaaaag ggaactttat cgatgccgga
     2521 aaagagtatg agaaggcggg agcccagttc ggcttcttgt acgattgcta caagctggct
     2581 ctgaacgtca ccggtggaac gtattacaag aagaacgtgt gtctgatgct gcgcggcatg
     2641 gcgcgcgccg gggcgatcgc cgagcggctc ggcaacggcg atacggatgt cgaccaggaa
     2701 atcaaggacg cgcgcgccga tctgttgatc acggcggata ttccggccga tctcaatcag
     2761 gcccgcacgc aggcgaaaga cgaactcgac cgcatcgaga agatgcagcc gctcgtgatg
     2821 ctctgcaaag atggcaagta cattcagatc atggaacacc tcgactccat gatcaagggt
     2881 ggaaccgtgc cgttcggaga aatggtcggc atgccgacct gctacgtgct ctccgagtgc
     2941 gcggtcaatc tcaagaccgc cgatgcgatc gagacggcga aaaagatctg ctttctgcat
     3001 tcgcagaagt tcaaataccc gttgttccag aagctggcat tcaaggtgac cagtctgacg
     3061 gccgaaaacg tggccgtgaa accgagcgcg gcgaagccga ccgcaaccgc tgccaagccc
     3121 gcgggtgacg cgaaagagcg tttcaagctg gccatgtcgt atgcgtcaaa gaaagacttc
     3181 cagaaagcga tgccgctgtt cgagtcgtgc tacaaggaca acccgaagaa cgtcgatttc
     3241 gtctacttct tcgcgttagc ccagagcaaa gcgggcaagc ccgacgaggc gcagaaactg
//
LOCUS       DAORHD010000457         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_32739_length_3299_cov_3.175401, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000457 DAORHD010000000
VERSION     DAORHD010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32739_length_3299_cov_3.175401"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKO06_10410"
     CDS             complement(<1..299)
                     /locus_tag="PKO06_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70101.1"
                     /translation="MEHIQIKFADEKGRIRESVFETESLPLLRAALQERGVFILSETP
                     VPRRFTEVVASWFPFSGGASLSDVAEFTQLFKTLLKAGLPLRDALDALIDEQANSP"
     gene            complement(303..1067)
                     /locus_tag="PKO06_10415"
     CDS             complement(303..1067)
                     /locus_tag="PKO06_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV70102.1"
                     /translation="MSRTLILSDIHSNFDALTQVLRDCQSMPVDRTWVLGDICGYGPQ
                     PQECIGAIQALSHPCIVMGNHDRVIGKIEQPVGFNPHAISAAYRNMRQLDDSSIQWLA
                     QLPARVEVDAHTVLCHGSPEDPDMYLLSLAAAVSSFHQLKNENRLLGFFGHTHVPAVF
                     EWDQETNAFNDIEIEYGQPVTLDLSGRFRYLINPGSVGQPRDGNPMASYAILIQEHGR
                     GEICFVRVGYSVVECQRKMSQLGYPEMLIKRLAVGF"
     gene            complement(1107..1757)
                     /locus_tag="PKO06_10420"
     CDS             complement(1107..1757)
                     /locus_tag="PKO06_10420"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV70103.1"
                     /translation="MTRLIKQEFQCPVCRAAFQGQVVESVSHQGQDSDFFPHYLGPDP
                     LPFFLVQCVQCQYCAYPEDFTEESETAKLPTPKQIREILETPLAKKMDPEARRYFLAG
                     RLYELGHKNPYHIGNLYLRGSWCCRIVGDRKGEVEMQQLAVRFLRLSAEQSTMTNPEN
                     LPVVIYLVGELYRRLDEKNQAREWFGNVEEAILDAEQQWLVELAKKQAELNEHFIN"
     gene            complement(1853..2374)
                     /gene="lepB"
                     /locus_tag="PKO06_10425"
     CDS             complement(1853..2374)
                     /gene="lepB"
                     /locus_tag="PKO06_10425"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV70104.1"
                     /translation="MEHESVKDWTKDFFKALVFFVFLQTFLMQGFVIEGACMEPQLKS
                     REKIMVNKLIYHLKRPHVGEVVVFPYPLDRKKDFIKRVVGVAGDVIEVRDGYLYRNDR
                     RINEPFVKEYIFGTYGPVLVPEGKVCVMGDNRNNSHDSRSWGLLEIEQVKGRAEFKFW
                     PIWATGWIPVNPG"
     gene            2737..>3299
                     /locus_tag="PKO06_10430"
     CDS             2737..>3299
                     /locus_tag="PKO06_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70105.1"
                     /translation="METTHTLLQQVTKALNRVLCGKPEVVELGLTTLIAGGHLLLEDL
                     PGVGKTTFGSALARCVNGSFSRIQFTADLLPSDVLGCRVFYRSQEKFELVRGPIFSNL
                     VMADELNRAPPKTQSALLQAMSEGQVSIDRETLSLPTPFMVIATQNPRWHQGTYPLPE
                     SQRDRFLMRLSIGYPPPAVEMTILQDR"
BASE COUNT          736 a          982 c          840 g          741 t
ORIGIN      
        1 ggggagttgg cctgctcgtc gatcagggca tccagagcgt ctcgcaaggg caaacccgcc
       61 ttcaggaggg tcttgaacaa ttgcgtgaat tccgccacgt cgctgagtga agcaccaccg
      121 gaaaacggga accaggacgc cacgacttcc gtgaaccttc ggggaacggg ggtttcggaa
      181 agaatgaaca ctccccgttc ctgcagagcc gcgcgcagca acggcagcga ttcggtttcg
      241 aagacgcttt cccgaatgcg tcctttctcg tcagcaaact tgatctgaat gtgttccatt
      301 gtttagaaac ccacagccag tcttttgatc agcatttccg gataccccaa ctgggacatc
      361 ttccgctggc actcgacgac cgagtagccg acacgcacga agcagatttc gccacgtcca
      421 tgttcctgga tcagaatagc ataggatgcc atcggattgc cgtctcgagg ttgcccgacc
      481 gaacccgggt tgatgagata ccgaaagcgg ccggaaagat caagcgtcac tggctggcca
      541 tattcgatct cgatgtcatt gaacgcgttg gtttcctggt cccattcgaa gaccgccggg
      601 acatgggtat gaccaaaaaa accgagcagt cggttctcgt tcttcagctg atgaaacgag
      661 gaaaccgcag ccgccagaga cagcagatac atgtccgggt cttccggact cccgtggcaa
      721 agcaccgtgt gcgcatcgac ctcaacgcgc gcgggcaact gtgccagcca ttgaatgctc
      781 gagtcgtcca gttggcgcat attccgatag gcagcggaga tcgcatgagg attgaaccca
      841 acaggttgct cgatcttgcc gatcacccga tcgtggtttc ccatgacaat gcatggatgc
      901 gacaaagctt gaatcgcccc aatgcattcc tgcggctgcg gaccataccc gcaaatgtcg
      961 cccaggaccc aggttcgatc gacaggcatg ctctgacaat cacgcagcac ctgagtcagg
     1021 gcgtcaaagt tcgagtggat atctgagaga atcagcgtcc tggacaaagg gttcctcccg
     1081 gtgacgcccg tcagcagtca cggccctcaa ttgatgaaat gctcgttcaa ctccgcctgt
     1141 ttctttgcca gttcgaccag ccattgttgt tcagcatcga gaatcgcttc ttccacgttc
     1201 ccaaaccatt ctctcgcctg gtttttttcg tcaagccgcc ggtacaattc cccaaccaga
     1261 tagatgacca caggcaggtt ctcaggattt gtcatggtcg actgctcggc tgagagccgc
     1321 aggaagcgga cggccagctg ctgcatctct acttcccctt tccggtcgcc aacaattcga
     1381 cagcaccatg agcctcgaag atagagattg ccaatatgat acgggttctt gtgtcccagt
     1441 tcgtacaacc gccctgccag aaagtatcgt cgggcctcgg ggtccatttt tttcgccaga
     1501 ggggtttcga gaatctcgcg tatctgcttt ggagtcggca gtttcgcggt ctcagattct
     1561 tcggtaaagt cctcggggta ggcgcaatac tgacattgca cgcattgaac cagaaagaac
     1621 gggagcggat ctgggcccag gtagtgcgga aagaagtcgg agtcctggcc ctgatggctg
     1681 acggattcca ccacctgccc ctgaaacgcg gcacgacata cggggcattg gaactcctgc
     1741 ttgatcagac gagtcacagt ctatctcctg gaacgacgtt gcacgacgac cacatgggct
     1801 tctacccggt cagcgcttga ttatatggcg gtcccggagg gggtgtcaat cttcaccccg
     1861 gattaacggg aatccatccg gttgcccaga tcggccagaa tttgaactcg gcccgccctt
     1921 tcacctgttc gatttcgagc aatccccagg atctgctgtc atgcgaattg ttgcgattgt
     1981 cgcccatgac gcacactttt ccctcgggaa ccaggaccgg accgtacgtc ccaaagatgt
     2041 attcttttac gaacggctcg ttgatgcgcc gatcattacg atacagatag ccgtcccgca
     2101 cctcgatcac atctcctgcc actcccacga cgcgcttgat gaaatccttc ttgcggtcga
     2161 gcggataagg aaagaccaca acttcgccga catgcggcct cttcaagtgg tagatcagct
     2221 tgttgaccat gattttttcg cgtgacttga gctgcggctc catgcacgca ccttcgatca
     2281 caaagccctg catcagaaat gtctgcaaaa aaacaaaaaa gacgagtgct ttgaaaaagt
     2341 ctttggtcca gtctttcacc gattcatgct ccatgagata cgcaccttga gaaccagcag
     2401 gctacgatct tcgaggtacc catttcggac gaatgcgtgg ttcttgttag tcaaaacccg
     2461 aaaaaaaatg cgcacttgtg ttaccctttt ctgcaggaca cttttgtcat gaatgcccat
     2521 tactgccggt cgtcctgcgc gttttgccag ccgctccatc ctcacggtcg attccgagcg
     2581 acgatctgtt tcgtagtcgg cctgatgttc atgccgtggt tctcgctttc cgcttcggca
     2641 gccctgcctg acgtcgactt ccaactgcag aaatccgatc agaacggcat ctgagcacca
     2701 accaatcgaa atcatcccaa ccaacaccgt aagcccatgg aaacaacaca cactctcctg
     2761 caacaagtga caaaagctct gaatcgcgtt ctctgcggca aacccgaagt ggttgagctc
     2821 gggttgacca ccctgatcgc cggcggacat cttctgctcg aagaccttcc gggggtcggc
     2881 aaaacaacct tcggctctgc cctcgctcgc tgcgtgaacg gctcattctc tcgtatccag
     2941 ttcacggcag atctcctgcc atcggacgtg ctcggatgcc gggtcttcta ccgcagtcag
     3001 gaaaagtttg aactggtgcg aggcccgatc ttctccaatc tcgtcatggc cgatgagttg
     3061 aatcgtgcgc ccccgaagac gcagagcgcg ttgctgcaag ccatgagcga aggtcaggtc
     3121 tccatcgacc gagaaacgtt gtctctgcca acgccattca tggtcatcgc cacccagaac
     3181 cctcgctggc accagggaac ctatcccctt cccgagtctc agcgcgatcg atttctcatg
     3241 cgcctgtcga ttgggtatcc tcctccggcg gtcgaaatga cgattcttca ggatcgcca
//
LOCUS       DAORHD010000458         3280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33036_length_3280_cov_3.209922, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000458 DAORHD010000000
VERSION     DAORHD010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33036_length_3280_cov_3.209922"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="PKO06_10435"
     CDS             <1..1781
                     /locus_tag="PKO06_10435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNV70106.1"
                     /translation="RLQFREQMRRDLARATEDLVKLPTLDLAHLASWEKALRIYEDLT
                     ARSWHYSTYIGCLTADDANNTAYQAEAAEDAQTGAEMQKLTTQFKRGFRTADDASFAA
                     LLKRPSLRGAEHHIRRLREEAKRSMATELENLAADLEVEGYSAWGRMYDQLIGKLEFE
                     LVRPDGKTEMRPFSEYETLLGDADPALRKAAFVGLTAAVQKLADPISHALNHIAGWRL
                     TLNRYRGIPDILDNALFETDIRRETLDAMFAALKKHIDIPRSMARIKAKKLGQQHLGW
                     QDFDAPLPETSHARISWKEGHQKLLRAFRDRLPEMADLMEEMSQKRFLEAEIRSGKAP
                     GAFCTDSPLTGESRIFMTYGGTLSDLYIMAHESGHAYHNHVMKDLRPSARLMPNTLAE
                     TASTFGELLCTYGLLADPTTPAEQKARILNMEATNTIGYLLDVPCRFFFEKALYAERA
                     KGEVSVERLQELMLTAQRDMFGDTLDPAGNPWFWATKGHFYFPDSTFYNFPYTFGFLL
                     SRGLMAEYQKQGPSFLPRYREFLRLSGSMPAEDVARQTLGIDLSKSDFWENAILSLQE
                     FCDLYDKTPVASRPTRPHTSPGASGR"
     gene            complement(1868..2893)
                     /locus_tag="PKO06_10440"
     CDS             complement(1868..2893)
                     /locus_tag="PKO06_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70107.1"
                     /translation="MSQKHQRSRDQSVSPEIVDEGSVATGETVKPVAQTSGLQVSVGS
                     WSSTEPVFDGKEETFLFPHRRQSRKPVVASPILVQPPADVECLFTAGLLTAGPGRIKI
                     DLLTPLEQAFVASGFLGGTLLLFAAIKSGGVPAPDLLTFLGILFLVVGGLGFKYTDNY
                     YVVDVLRRVVSYHFQFLHLEQETIMADTRSMACVTSHAELRRDRGEYWFEYATALIMK
                     DGTLFPVSDYQRDGLLMANHRARLVAGYLGLPFVSGCENADVIVTRHDSSGDVQVAHR
                     LIDVTALNSPVSADVAAGTKQIQEHWMRSLMAVPAIMAGVLFIMTYGPYFFLLVLLFV
                     LRTLLLH"
     gene            3086..>3280
                     /locus_tag="PKO06_10445"
     CDS             3086..>3280
                     /locus_tag="PKO06_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70108.1"
                     /translation="MTSNLKSQNLSIMMTDIQGYTNTSSGCSREEIVELIRRHNKLMV
                     PVIQFYGGTIIKSIGDAFLCT"
BASE COUNT          780 a          988 c          872 g          640 t
ORIGIN      
        1 cacggctgca gttccgcgag cagatgagac gcgatctggc ccgggcaacc gaagacctcg
       61 tgaagttgcc caccctcgat ctggcgcatc tcgcctcgtg ggaaaaagct ttgcgtatct
      121 acgaagatct gaccgcccgc tcctggcact actcgacgta tatcggctgc ctcaccgcgg
      181 atgacgccaa caacaccgcc tatcaggccg aagcggcaga ggatgcacaa accggcgccg
      241 agatgcagaa actgaccaca cagttcaaac gcgggtttcg aaccgctgac gacgcgtcgt
      301 tcgcggctct gctcaagagg ccgtcgcttc gcggcgccga gcatcacatt cgtcgcctgc
      361 gcgaagaggc caagcgttcg atggccacgg aactcgaaaa tctcgctgcc gacctggaag
      421 tcgaagggta ttcggcctgg ggccgcatgt atgatcagct gatcggtaaa ctcgagttcg
      481 aactggtgcg ccccgacggc aaaaccgaaa tgcggccctt ctcggagtat gaaaccctgc
      541 tgggagacgc tgacccggcc ctgcgcaaag ccgcgtttgt cggtctcacc gcagcggtgc
      601 agaaactcgc cgacccgatt tcacacgccc tcaaccacat cgccggttgg cgactgacac
      661 tcaaccggta tcgaggcatt ccggacattc tcgacaacgc ccttttcgaa accgatattc
      721 ggcgtgaaac actcgacgcc atgtttgcgg cactcaaaaa acatattgat attcctcgat
      781 caatggcccg tatcaaggcg aagaaactag gacaacaaca cctgggctgg caggatttcg
      841 acgctccgct gcccgaaact tcgcacgccc gcatttcctg gaaagaagga caccagaagt
      901 tactccgagc ctttcgtgac cgcctgccgg agatggcaga tctgatggag gaaatgtccc
      961 agaagcgatt tttagaagca gagattcgaa gcggcaaagc accgggggct ttctgcaccg
     1021 acagcccact gacgggcgaa tcgcgcatct tcatgaccta cgggggcacg ctgagcgatt
     1081 tgtacatcat ggcgcatgag tcaggtcatg cgtatcacaa ccatgtcatg aaagacctgc
     1141 gtccatcggc ccggttgatg cccaatacgc tggccgagac cgcatcgacg ttcggcgaac
     1201 tgttatgcac ctatggcctg ctggcagacc ccaccacgcc agccgaacag aaagcgcgca
     1261 ttctgaatat ggaagccacc aacacgatcg gctatctgct cgatgtgcca tgccgatttt
     1321 tcttcgaaaa ggctctgtat gccgagcgtg ccaagggcga agtcagtgtc gagcgcctgc
     1381 aggaactgat gctgacggcc cagcgcgata tgttcggcga caccctcgat cccgccggga
     1441 acccctggtt ctgggccacg aagggacact tttactttcc cgacagcacg ttctataact
     1501 ttccgtacac gttcggtttt cttctcagcc gcgggctgat ggctgagtat cagaagcagg
     1561 gcccatcctt tctgcccaga tatcgcgagt ttttgcgact cagtggcagc atgccggccg
     1621 aggatgtcgc cagacaaact cttggaatcg atttgtccaa gtctgatttc tgggaaaacg
     1681 cgatactgag cctgcaggaa ttctgtgatc tctacgataa aacacccgtg gcttctcgac
     1741 cgacacgacc ccacacctct cccggcgcca gcggacggta aatcaggggg taagggagaa
     1801 agcggggagc acggcgcagg acggtcatcc agccctgtgt ttcagaatac cgcttttccg
     1861 ttttccgtta gtgcagcaga agagtccgca gcacgaagag caaaaccagc aggaagaagt
     1921 atgggccata cgtcatgatg aacaatactc cagccataat tgccggaaca gccatcagcg
     1981 agcgcatcca gtgctcctga atttgttttg taccagcagc aacgtctgcc gacacaggtg
     2041 aattgagggc agtcacatct atgagccgat gcgcgacctg cacgtccccg ctcgaatcat
     2101 gccgagtcac gatcacatca gcattctcgc aaccgctcac gaagggcaga ccaaggtagc
     2161 ccgccaccag tcgggcgcga tggtttgcca tgagcagacc gtctcgctga taatccgaca
     2221 cgggaaagag cgttccgtct ttcatgatca gggccgtcgc atactcgaac cagtactctc
     2281 cgcgatcgcg tcgcaactcg gcatgactgg tgacacaggc catcgaacgc gtgtccgcca
     2341 taattgtctc ttgttcgaga tgcagaaatt gaaaatgata cgacacgacg cgacgaagca
     2401 catcgaccac gtaatagttg tccgtgtatt tgaagcccag acctccgacc accaggaaca
     2461 ggatccccag aaaggtcagc agatcagggg cgggaactcc gccggacttg atcgccgcaa
     2521 aaagcagaag cgtgccgccc agaaatcccg aggcaacgaa tgcctgctca agtggtgtca
     2581 gcagatcgat tttgattcgt ccaggtccgg ctgttagtaa accagcggtg aacagacact
     2641 ccacatccgc cgggggttgt acaagaatcg gagaagccac aaccggtttt cgactctgac
     2701 gacgatgggg aaaaagaaac gtctcttctt tcccgtcgaa aacgggctcg gtggaactcc
     2761 aggaaccaac agacacctga agtccgctcg tctgtgcaac gggcttcacg gtttcaccgg
     2821 tggcgacgga accttcatcg acaatctctg gtgaaaccga ctgatctctc gaccgctgat
     2881 gcttctggct catcaggtaa cactcctgtc gttgcacgtc cacccgcaac cccgacctgc
     2941 tcagcctcgc ttcgttcctg ccgacgagct tcggccagct gctgattcgg acaacatctt
     3001 gtgaggcggg tgtcagcatg ctaccatatc cgacgaatgg aatacccagc ggtatcgttc
     3061 agtcacccgt gcgaaggaga ggaccatgac cagtaacctg aagtctcaga acctgtcgat
     3121 catgatgacc gacattcagg gctataccaa caccagttca ggatgttccc gcgaagagat
     3181 cgtcgagctg attcgccggc acaacaaact catggttccg gtcattcagt tttatggcgg
     3241 caccatcatc aagtccatcg gcgacgcctt cctctgcacc
//
LOCUS       DAORHD010000459         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33088_length_3277_cov_2.889820, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000459 DAORHD010000000
VERSION     DAORHD010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33088_length_3277_cov_2.889820"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /locus_tag="PKO06_10450"
     CDS             complement(<1..1721)
                     /locus_tag="PKO06_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70109.1"
                     /translation="MRIVLFWVSLIAALATIFAAASGGYLYLAASQSLEAEGQRWGWE
                     RLEVATRQLNELCSRLNTGALALANDWNSGRVGPEELPGRLREYLAQTPEALSIGLLF
                     PPHQQSDLAWRHAPFYLQRENLDLETADLLPIIVRPLERITASGTIRQTGTLVVEISR
                     RRVRELLLDLDLGRHAYAFLLDDNGRFIWHPHQELITSGKTIFSYSEETHEASLGRFG
                     RAAVEGQRSLQVINSPLTGLRSWAYCGPVQQNHWSLCLMVSSRDLTAPSLPMQQRLFR
                     VVIYATAALCLLFCAWYLRFEDFADHVWKLGTIVSLVLCLGIAGIWAYTTLYFEARFP
                     GSAPLLETSFLAKIETEHIERSRKQGYDDPLFIPSGLFIQSVEFESAINVKLKGYAWQ
                     KYPIPMIASISEGFIFPEAVETSLEKAYEREENGIRTIGWSFSTTLRQSFDYSTYPFD
                     QQNIWIRMWHQDFDRNIVLVPDLNGYNWLNPSMKPGIDEHIILPAWNIEKSYFNYNLL
                     DYNTNFGINSYNGQDRFPELHFNMLIRRRFLDPFMSSIVPLLVVALQMFSVLIISTKK
                     EDLANRMG"
     gene            complement(1744..>3277)
                     /locus_tag="PKO06_10455"
     CDS             complement(1744..>3277)
                     /locus_tag="PKO06_10455"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HNV70110.1"
                     /translation="KFASDSTLLTIIGSLGSANGDFSSPSGICVDAGDNVYVADTDNH
                     RIQLFTSSGVHQMSFGVNGSSDGQFIRPEGVGVAPGGEIFVADTGNHRIQRFDAGGAP
                     LGWWGRDDLGVTGWHAVGSGRVSRAGSADLEFDSPKSVVHDGGNSLIIGDTGNNRVQV
                     LSLLTGTATIIGTGGTQLGQFDYPTSVAVDGANFWVADYANHRAQQFSPIGAQLAARV
                     PDTSGLNTSAERVAIDANTGLIYVSDLSEGVISVFQPDGSFVRRIGAWGTGPGQMREP
                     RGLVFAADGTLWVADSGNGRLIRFTTDGTVLQTLGTFGTGDGQFRSPWAVEIASEGHL
                     VVADTDNNRVQIFLPDGTFERSIGTPGSGNGQLDRPGGIAVSSDGRIYVADTGNARIQ
                     RFAWDGAYQGWWGADVGGGVGWHGTATAVTAQPDIGPCRFNAPTDLRVDDEGCVYVTD
                     AVAGTLQKFSPQQSSEPLAGHILTLDLNTTNIRGTAISLSGQLVLTATDQWIRLFDPG
                     LR"
BASE COUNT          708 a          908 c         1023 g          638 t
ORIGIN      
        1 cccatgcggt tcgccagatc ttctttcttg gtgctgatga tcaggaccga gaacatctgc
       61 agggcgacga ccagtaacgg aacgatggag gacatgaagg gatcgagaaa acgacgccga
      121 atcagcatgt tgaagtgaag ttcggggaaa cgatcctggc cattgtacga gttgatgccg
      181 aagttggtgt tgtagtcgag caggttatag ttgaagtaac tcttttcgat gttccaggcg
      241 gggaggatga tgtgttcgtc gatgccgggt ttcatggaag gattcagcca gttgtagcca
      301 ttcagatcgg gcacgagaac gatgttgcga tcgaaatcct gatgccacat ccgaatccag
      361 atgttctgct gatcgaaggg gtaggtcgag taatcgaagc tctgacgcag cgtggtcgag
      421 aagctccaac cgatggtacg aatgccgttt tcctcgcgct catacgcctt ctcaagactg
      481 gtttccaccg cctcgggaaa gatgaagccc tctgagatgc tggcaatcat ggggatcgga
      541 tacttttgcc aggcataccc cttaagcttg acgttgatcg cgctctcgaa ctcgaccgac
      601 tgaatgaaca ggccggaggg gatgaacaga gggtcgtcgt agccctgttt ccgcgatcgt
      661 tcgatgtgtt cggtttcgat cttggccaga aaggaggtct caagtaacgg agcgctgccc
      721 gggaagcggg cttcgaaata gagcgtggtg tatgcccaga tgccggcgat tccgaggcac
      781 agaaccagtg agacgatcgt tcccagtttc cagacgtggt cggcgaagtc ctcgaatcgt
      841 agataccagg cacagaaaag caggcagaga gcggctgtcg cataaatcac gacgcggaac
      901 aaccgttgtt gcatgggtaa cgaaggcgcg gtcagatcgc gagacgacac catgaggcag
      961 agtgaccagt ggttctgctg taccggccca cagtaagccc aggaccgcag cccagtcagt
     1021 ggagaattga tcacctgaag ggaccgctgc ccttccacgg cggcacgccc aaaccgaccc
     1081 agtgaggctt cgtgggtttc ttcgctgtag gaaaagatcg ttttgccgga agtgatcagt
     1141 tcctggtggg gatgccagat aaaacggccg ttgtcatcga gcagaaaggc ataggcgtga
     1201 cgtccgagat cgaggtcgag cagaagttcc cgcacacgtc ttcgcgagat ctcgaccacg
     1261 agagtcccag tttgtcgaat ggttccagag gcggttatgc gctcaagagg gcgaacaatg
     1321 atcggaagca ggtcggctgt ttccagatcg agattttccc gttgcagata gaatggggca
     1381 tgacgccagg ccagatcgga ctgctgatgc ggagggaaca gaagcccgat cgacagcgct
     1441 tctggagttt gcgcgagata ctcgcgaaga cgaccgggaa gctcctccgg cccgacgcgt
     1501 ccactgttcc agtcgttcgc cagagccaga gcgccggtgt tgagtcgcga acagagttcg
     1561 ttcagttggc gcgtggcaac ctccaggcgt tcccaacccc aacgttgccc ttccgcttcg
     1621 agcgattggc tggcggcgag atacagatag ccgccggaag cggcggcaaa aatggtagcc
     1681 agggcggcaa tcagtgaaac ccagaagaga acgatgcgca cggaaaattc ctttctgacc
     1741 cgtctagcgg aggccggggt cgaacagacg gatccattgg tcggttgccg taagtacgag
     1801 ttgtcctgag agcgaaatag ctgtaccacg tatattggtc gtattgagat ctagagtcag
     1861 aatgtggccg gccagaggtt cgctgctttg ctggggcgaa aacttctgca gcgtaccggc
     1921 gacggcatcg gtgacgtaga cgcagccttc gtcgtcgacg cgcagatcgg tcggagcgtt
     1981 gaagcgacag gggccgatat ccggttgggc tgtcactgcg gtggctgtcc catgccagcc
     2041 gacgccgccg ccgacgtccg ccccccacca gccttgatag gcaccatccc aggcaaaacg
     2101 ctggattcgg gcgttcccgg tatcggccac gtagatgcgg ccgtctgagg aaacggcaat
     2161 gccgcccgga cgatcaagct gcccgttgcc tgagccgggg gtgccgatgg agcgctcgaa
     2221 ggtcccgtcg gggaggaaga tctggacacg gttgttgtcc gtgtccgcga ccaccagatg
     2281 cccttctgag gcgatctcta ccgcccacgg tgagcgaaac tggccgtccc cggtaccgaa
     2341 ggtgccaagg gtctgcagaa ccgtgccgtc ggtcgtgaac ctgatcagac gcccgttgcc
     2401 tgaatcggcg acccacagtg tgccgtcggc ggcaaaaacc aggccgcgag gctcgcgcat
     2461 ctggccaggg cctgtccccc aggctccgat gcggcgaaca aagctgccgt cgggttgaaa
     2521 gacgctgatg accccttccg agagatccga gacatagatc aggccagtgt tagcatcgat
     2581 tgccacccgt tcggcagaag tgttcaggcc actcgtgtcg gggactcgtg cggcaagctg
     2641 tgctccgatc ggcgagaatt gctgggcgcg gtggttggca tagtctgcga cccagaaatt
     2701 tgcaccatcc acggcgaccg acgtggggta gtcgaactgt cccagttggg tgcctccggt
     2761 tccgatgatg gtcgcggttc cggtgagcag ggaaagcact tgaacgcggt tgttcccggt
     2821 gtcaccgatg atcaggctgt tcccgccgtc atgaaccacg cttttggggc tgtcgaactc
     2881 gagatcggct gacccggccc ggctgacccg gccggatccg accgcgtgcc atccggtgac
     2941 tcccaggtca tctcttcccc accagcccag cggcgcaccg ccggcgtcaa aacgttgaat
     3001 gcgatgattc ccggtatcgg ctacgaagat ctcgccgccc ggagcgacac ctaccccttc
     3061 aggcctgatg aactgcccat cactgcttcc attgacgcca aacgacatct gatgaacacc
     3121 ggaactcgtg aacagttgaa tacgatgatt gtcggtatcg gcgacgtaca cgttgtcacc
     3181 cgcgtcgacg cagattccag aaggagaaga gaagtcgccg tttgccgaac ccagcgaacc
     3241 gatgatggtc agcagggtgc tgtccgaagc gaatttc
//
LOCUS       DAORHD010000460         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33118_length_3275_cov_2.556832, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000460 DAORHD010000000
VERSION     DAORHD010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33118_length_3275_cov_2.556832"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2729)
                     /locus_tag="PKO06_10460"
     CDS             complement(<1..2729)
                     /locus_tag="PKO06_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HNV70111.1"
                     /translation="MSLHKEICFESEICKHLGDHGWQYAEGDASQYDRARALFPVDVL
                     SWVQATQSESWQALTKNHGAKAEETLLNRLRDQIDKRGTLDVLRHGIELLGLKKPLMM
                     AQFKPALVINTGILARYHANRLRVVRQVKYSVNNENCLDLVLFLNGVPVATAELKTDF
                     TQSVTDAIDQYRFDRQPRPKGQTPEPLLSFPNGALVHFAVSNSEVHMTTRLAGAATRF
                     LPFNQGDNGAAGNPVNPKGHRTAYLWEHVWERESWLEILGRYIIAQRDKKKQIEKIIF
                     PRFHQLDVTRKLQTAIRAEGAGGKYLIQHSAGSGKTNSIAWSAHFLAELHNDQHQKIF
                     DTVLVVSDRNVIDAQLQEALFDFQRTTGVVETITNKEGSKSGKLAEALSGDKKIVVCT
                     IQTFPFAIETVQKLAATKGKRFAVLADEAHSSQSGEAAARLREVLSPEELKELEDGGT
                     VSTEDILAAQMAARADTRGITFVAFTATPKTKTMELFGTRPDPTRKPAPDNIPTPFHV
                     YSMRQAIEEGFILDVLQNYTSYKLAFKLAHEGSTIDESQVERNKALKRIMKWVKLHPY
                     NIAQKVQIVVEHYREFVAPLLNGLAKAMVVVGSRVEAVRWKLAIEKYIKDCGYKIGTI
                     VAFSGEVTDTESGPDGFTENSHTLNPNLRGRDIREAFKGEEYQILLVANKFQTGFDQP
                     LLCGMYVDKRLAGIQAVQTLSRLNRAFPGKDTTYVLDFVNEAAEVLASFKMYHTTAEL
                     ASTTKPELVLDLRAKLDAAGQYDEPEVDRVVEAELKGASQSDLVSAIVPVQDRIMKRY
                     KAAQEAFFNAKGAKNTKAEKAAKDEMDALVLFKTDMGSFVRLYTFLSQIFDYGNTAFE
                     KRAIFYKRLIPLLDFGSEREGIDLSKIVLTHHRLHDGGKKPMPLPEGEKILLPP"
     gene            complement(2726..>3275)
                     /locus_tag="PKO06_10465"
     CDS             complement(2726..>3275)
                     /locus_tag="PKO06_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV70112.1"
                     /translation="TICTNQRMHLFGEIVDGRMRLNEFGEIVVAEWLKTPTIRAEIEL
                     DEWVVMPNHFHAIVCIRRGDRPVAPTVAPPPRPVARPGAANHAAHGPRPKSIGAMIAG
                     FKSGVTTCINTKRGSPGAKVWQRGYYEHIIRDNESMGRIREYITMNPMKWGSDRENNQ
                     RQAETPGRSSFRVAEEAAPWET"
BASE COUNT          610 a          980 c          927 g          758 t
ORIGIN      
        1 ggcggcagga gaatcttctc accctcaggc agaggcatcg gcttctttcc gccgtcatgc
       61 agccggtggt gggtcagcac gatcttcgag aggtcaattc cctcgcgctc acttccgaaa
      121 tccagaagcg gaatcagcct cttgtagaag atggcgcgtt tctcgaacgc cgtgtttccg
      181 taatcgaaga tctgcgacag aaaggtatac agccgaacaa acgaccccat gtcggtcttg
      241 aagagaacca gtgcatccat ctcgtccttc gcggcctttt ccgctttggt gttcttcgcg
      301 cccttggcgt tgaagaaggc ttcctgagcc gctttgtatc gcttcatgat gcggtcctgc
      361 accggcacga tcgccgacac cagatcgctc tgactggctc ccttgagttc ggcctcgacg
      421 acccggtcaa cctcgggctc gtcatactga ccggcggcat cgagtttcgc gcgcagatca
      481 agaaccagct cgggcttggt ggtcgaagcg agttcggcgg tggtgtgata catcttgaac
      541 gaggccaaca cctcggcggc ctcgttcacg aaatccagaa cgtaggtcgt gtctttgccc
      601 ggaaacgcgc ggttcaggcg cgagagcgtc tgcacggcct gaatgcccgc cagccgttta
      661 tcgacataca tgccgcacaa cagcggctgg tcaaaaccgg tctggaactt attggccacc
      721 agcagaatct ggtactcttc gcctttgaac gcctcgcgaa tgtcccgccc gcgcaggttc
      781 gggttgagcg tgtggctgtt ctcggtgaag ccgtcggggc ctgattccgt atccgtcact
      841 tcaccggaaa aagcgacgat ggtaccgatc ttgtaaccac aatccttgat gtatttctcg
      901 attgccagct tccaccgcac cgcttcgacg cgactgccga cgacgaccat ggctttggcc
      961 agaccgttca acagcggggc gacaaactcg cggtaatgct cgacgacaat ctgcaccttc
     1021 tgggcgatgt tatacgggtg cagtttcacc cacttcatga tgcgcttcaa ggccttgttc
     1081 cgctcgacct gactctcgtc gatcgtactg ccctcatggg ccagtttgaa ggccagcttg
     1141 tatgaggtgt agttctgaag cacatcgaga atgaatccct cttcgatggc ctgccgcatc
     1201 gaatacacgt gaaacggggt cggaatgttg tcgggcgcgg gtttccgcgt cgggtccggt
     1261 ctcgtgccga acagctccat cgtcttggtt ttcggcgttg cagtgaacgc gacgaaggtg
     1321 atgccgcgcg tatcggctct cgcggccatc tgagccgcaa gaatgtcttc ggtgctgacc
     1381 gtgccgccgt cttccagttc cttcagttct tcgggcgaaa gcacctctcg cagtcgcgcc
     1441 gccgcctcgc cgctctgcga gctgtgcgcc tcgtcggcaa ggacagcgaa ccgcttgcct
     1501 ttggtcgccg ccagcttctg caccgtctcg atggcaaacg gaaaggtctg aatggtgcac
     1561 accacgatct tcttgtcgcc cgagagcgct tccgccagct ttccgctctt actgccttct
     1621 ttgttggtga tggtctcgac cactccggtc gttcgttgaa agtcgaagag ggcctcttga
     1681 agctgggcat cgatgacatt gcgatctgac acgacgagga cggtatcgaa gatcttctga
     1741 tgctggtcgt tgtgcagttc cgcgaggaag tgggcggacc acgcaatgga gttggttttt
     1801 cccgatccgg ccgagtgctg tatgagatac ttgcccccgg ccccttcagc acgaatggcc
     1861 gtctggagct tgcgcgtcac atcgagctga tgaaagcgcg gaaagatgat cttctcgatc
     1921 tgcttcttct tgtcacgctg ggcgatgatg tatcgaccaa ggatctcaag ccagctttcg
     1981 cgctcccaga cgtgttccca gagataggcc gtgcgatgac ccttcgggtt caccgggttg
     2041 ccggccgcgc cgttgtcgcc ctgattgaac ggcagaaagc gcgtcgcggc ccccgcgagt
     2101 ctggtggtca tgtgcacttc gctgttgctg acggcaaagt gaaccagtgc tccgttcgga
     2161 aaagagagca acggttcggg tgtctggccc ttggggcgcg gctgtcggtc gaaacgatac
     2221 tggtcaattg catcggtcac gctctgcgtg aaatcagttt tcaactcggc agtcgccacc
     2281 ggcacgccgt tcaggaacag caccagatcg aggcagttct cgttgttcac cgagtatttc
     2341 acttgccgca ccacccgcag gcggttggca tgatagcgcg ccagaatgcc ggtattgatc
     2401 accagcgcgg gcttgaactg tgccatcatc aaaggcttct tcaaccccag cagttcgatg
     2461 ccgtgccgca gaacgtcgag cgtgccgcgt ttgtcgatct ggtcgcgcag ccggttcaac
     2521 aatgtctctt ccgctttcgc gccgtggttc tttgttagag cctgccacga ctcggactgc
     2581 gttgcctgca cccagctcag aacgtcaacc gggaacaatg cccgcgcccg atcgtactgg
     2641 gacgcatcac cctcggcata ctgccagcca tgatcaccaa ggtgcttgca gatctcgctc
     2701 tcaaagcaga tttccttgtg aagactcatg tttcccaagg tgcggcctcc tctgcgacac
     2761 ggaatgatga acggccaggg gtctccgcct gccgctgatt gttttcgcgg tcactccccc
     2821 atttcatcgg attcatggtg atgtattccc gaatccggcc catggattca ttatcgcgaa
     2881 tgatatgttc gtaatacccg cgttgccaca ccttcgcacc cggtgatcca cgtttggtgt
     2941 tgatgcaggt ggtaacgccg gatttgaaac ccgcaatcat tgcgccgatc gatttcggtc
     3001 gtggcccatg cgcggcatgg ttcgccgcgc ccggtcgggc gaccggccgg ggtggtgggg
     3061 cgaccgtagg ggcgaccggc cggtcgcccc tacggataca aacgatggcg tgaaaatggt
     3121 tgggcatgac cacccattca tccaattcga tttcggcgcg aatggtgggg gttttcaacc
     3181 attcggcaac cacaatttcc ccgaattcat tcaaccgcat cctgccatcg actatttcac
     3241 cgaataaatg catgcgttga tttgtacaaa tggtg
//
LOCUS       DAORHD010000461         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33146_length_3273_cov_3.262896, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000461 DAORHD010000000
VERSION     DAORHD010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33146_length_3273_cov_3.262896"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKO06_10470"
     CDS             <1..479
                     /locus_tag="PKO06_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70113.1"
                     /translation="LTENKPSRMITTLAFLREHFRFVPDSVWLYVRNSSEYLIVVSGD
                     VVPENWRCLFSPERLINRGKGFSVGSQLQNSRENPVLLHVAPGYLLVFPANLEGTVLD
                     SLAGHQEPGNDKWATFAKMVGLQPLLALEIDTTETSEALHCWVHLFPSVKCSDFAA"
     gene            458..817
                     /locus_tag="PKO06_10475"
     CDS             458..817
                     /locus_tag="PKO06_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70114.1"
                     /translation="MQRFRCLIGRDLTKLQAFLVHDDARTLLLSQVIDVLRELANPDE
                     VAKANINTASLHENPLPAYLAALKAKIQGQSVFLEGPGLRQNQSLAAAAAIGVIDSLL
                     SRHLPADLVPILPDSRL"
     gene            969..1922
                     /locus_tag="PKO06_10480"
     CDS             969..1922
                     /locus_tag="PKO06_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV70115.1"
                     /translation="METRSFGNTGISVTALGIGTLTMSPLQRGLAPSEGARVILAALE
                     RGVRFVDTAQMYGSYPHVALALADWGGETITVTTKSTARTSTEMLAAIEQARQEMNLD
                     IIDGFLLHAVRGAEDLQERESAIEALQKAKERGVVRAIGASTHSVNALQVLVDDPRIE
                     ILHPIVNQVGFGILDAGLDETMRLLTRAKSLGKGVYAMKAMGGGHLRHRSVEVLHWAL
                     TQKQIDAAAVGMTSLDEVEMNVAIAEKRPISDELMQRVSSQSRKLFVNEALCKGCGEC
                     VRFCEHEALNIEGGKAHPNPRRCVLCGYCAPHCPHFAIRVI"
     gene            2275..3051
                     /locus_tag="PKO06_10485"
     CDS             2275..3051
                     /locus_tag="PKO06_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70116.1"
                     /translation="MILSQKADLTETEQQELAELKAKSAEIKTALGIPTKAEPPMVAA
                     EPCPQNGDKPVCNKDGSCPSKKFRRHHGKHGKYQRRDGCKCSCQKMDCGKTCSTDNCP
                     CGCKGDPAKCVCKEKKSCKGKCPAMKGCEGRKDYHRYKKGCCKKGMCDKAKACASKCP
                     AMKAKCGDQKMVCKPCGKVDCACGCKGDATKCVCKEKKTCKGKCPAMKGCEGRKDYHR
                     YKKGCCKKGMCDKAKACASKCPAMKAKCGDKKMVCEPCGK"
     gene            complement(3123..3230)
                     /locus_tag="PKO06_10490"
                     /pseudo
     CDS             complement(3123..3230)
                     /locus_tag="PKO06_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          811 a          892 c          910 g          660 t
ORIGIN      
        1 atctgacaga aaacaagccc agccgcatga ttaccacgct ggcgtttcta cgtgagcatt
       61 tccggttcgt tccggattct gtctggctct atgtgcgcaa ttcctcggag tatttaatcg
      121 tggtttcagg ggatgtcgtt ccggaaaact ggcgctgcct cttttctccc gaacggttga
      181 tcaatcgtgg caaaggcttt tccgtgggaa gtcaactgca aaactcgcga gaaaatccgg
      241 ttctgctgca tgtggctcca ggttatctgc tggtgtttcc ggcgaatctt gaaggaacgg
      301 ttctggacag cctggcaggg catcaggaac ccggcaatga taaatgggca accttcgcca
      361 agatggttgg gctgcaaccg ctgctggctc ttgagattga cacgaccgaa acttcagaag
      421 cgcttcattg ctgggttcac cttttcccct cagtgaagtg cagcgatttc gctgcctgat
      481 cgggcgcgat ctcaccaaac tgcaggcatt tctcgttcat gacgatgcga gaactctgct
      541 tctaagccag gttatcgatg ttctgcgtga actggcgaac ccggacgaag tggcgaaagc
      601 aaacatcaac accgcttctc tgcacgagaa ccccctgcct gcatacttgg ccgcgctgaa
      661 agccaagatc cagggacaga gtgtattcct cgaaggtccc ggactgcggc aaaaccagag
      721 tctcgcagct gcagcggcga tcggcgtcat cgactccctg ttaagccgac acctgcctgc
      781 agatcttgtg ccaatacttc cagacagccg tctctgacca acacggttcg gccacgtatc
      841 gccacttccg ctgcatttct gaacgtgcgg ctcgttctgc agccacacag caaatcgcat
      901 tgccacgata gaacgtcgtg cgctaccatg aaggcaccag actggctgtc cacctgaagg
      961 agcacaccat ggaaacgaga agcttcggta acacaggaat ttctgtcacg gcactcggca
     1021 ttggcacact gaccatgagt cccctgcagc gtggattagc gccatcagaa ggcgctcgcg
     1081 tgattctggc agctttggaa cgcggagttc gctttgttga taccgcgcag atgtatggct
     1141 cttacccgca tgtcgcgtta gctctcgcag actggggtgg tgagaccatc accgtgacca
     1201 ctaaatccac ggcacgaact agtacagaga tgctggcagc catcgagcaa gctcgtcagg
     1261 agatgaatct cgatatcatc gacggctttc tccttcatgc ggttcgcggc gctgaagacc
     1321 ttcaggaacg tgaatcagcc attgaagccc tgcagaaagc caaagaacga ggagtcgttc
     1381 gggccattgg tgccagcacg cattcggtca acgctctgca ggtgcttgtc gatgatccac
     1441 gaatcgaaat cctgcatccg atcgtcaatc aggtcggatt cgggattctc gacgccgggc
     1501 tcgacgaaac catgcgcctg ctcactcggg ccaaatcact tgggaagggc gtgtatgcca
     1561 tgaaagccat gggtggcggc caccttcggc atcgtagcgt cgaagtgctg cattgggcgc
     1621 tcactcagaa gcaaatcgat gctgcagctg tcggcatgac cagcctggat gaagtcgaga
     1681 tgaacgtggc tatcgccgag aagcgtccca tttcagatga gctgatgcag cgtgtcagct
     1741 ctcaaagtcg aaagctcttc gtcaacgaag cgctctgcaa ggggtgtgga gaatgcgttc
     1801 ggttctgtga gcatgaagcc ctgaatatcg aaggcggaaa agcacacccg aacccacgcc
     1861 gctgcgtgct ctgcggatac tgcgctccgc actgccctca cttcgccatt cgagtcattt
     1921 agaacagaca gcgcatctgg acccggcgtt ccgccagagg tcctgggaat tcgcaacgcg
     1981 tgaaacacat gaaaaatacc tgacttcccc gctgatcctc ccgggttttc gcccacaaat
     2041 tgtgtggcat ttgaagggtg caaagtggct tgttcagcca agttacaaaa cttgtgctat
     2101 agtagaggcg ctcgctagac aaatacaaca ccacatttca tggaggtata catgcgttca
     2161 aaatggttgt cggtcctcgt tctttgtttg ctttgttcga ttccgggctt cgcccaacag
     2221 acagacgtct ttgctgatca ggttcgcgcg ctgaaggaac tgaagacata gatcgtgatt
     2281 ctttcccaga aggccgatct gacagaaacc gagcagcagg aactcgctga actgaaagcc
     2341 aagtctgcgg aaatcaagac cgctctgggt attcccacca aagccgaacc cccgatggtc
     2401 gcagcagagc cgtgcccaca gaacggcgac aagccggtct gtaacaaaga tggctcgtgt
     2461 cccagcaaga aattccgccg ccatcatgga aagcatggga aataccagcg ccgtgacggc
     2521 tgcaagtgct cgtgccagaa gatggactgc gggaagacct gctcgacaga caactgcccc
     2581 tgcggctgca agggtgaccc ggccaagtgc gtctgcaagg aaaagaaatc ctgtaaaggc
     2641 aagtgcccgg ccatgaaagg ctgcgaaggc cggaaagact atcatcgcta caagaaaggc
     2701 tgttgcaaga agggtatgtg cgataaagca aaagcatgtg ccagcaaatg cccggcgatg
     2761 aaggcgaagt gcggtgacca gaagatggtc tgcaagccct gcggcaaagt tgactgcgcc
     2821 tgtggttgca aaggtgacgc gaccaagtgt gtctgcaaag aaaagaaaac ctgcaaaggc
     2881 aagtgcccgg ccatgaaggg ctgcgaaggc cggaaagact atcatcgcta taagaaaggt
     2941 tgctgcaaga agggcatgtg cgataaagca aaagcatgtg ccagcaaatg cccggcgatg
     3001 aaagcgaagt gcggtgacaa gaagatggtc tgcgaaccct gtggaaagta aacgaagaac
     3061 cgtgtaagta agacgggccc gccatctggc gggcctgttt catttctgcg cgatcgggag
     3121 cgttactcga atctcaacgc gtcgatcgga tcgagctgtg aagccttcca ggctggatac
     3181 agaccaaaga agacaccaac cacggcggag aaagcaaacg cccccgccgc agcccaccag
     3241 cagcaaggcc gtcagcgcaa gaatgtgcaa acg
//
LOCUS       DAORHD010000462         3271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33181_length_3271_cov_4.040112, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000462 DAORHD010000000
VERSION     DAORHD010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33181_length_3271_cov_4.040112"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="PKO06_10495"
     CDS             <1..1301
                     /locus_tag="PKO06_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNV70117.1"
                     /translation="PKEARWSHLQANAKQPSIGKLVDDAMVALERDNPRLKGVLPKDY
                     ARPGLDKHRLGELIDLIGTIGLGDAANRSKDILGRVYEYFLTQFASAEGKNGGQFYTP
                     GCIVRLLVGMLSPYKGRVYDPCCGSGGMFVQSEKFVLEHGGRIGDISIYGQESNATTR
                     RLAIMNLAIRGIEADFGPEHADTFRRDLHKDLKAQFVLANPPFNDSDWHRVDDDVRWQ
                     FGVPPKGNANYAWVQHFIHHLAPDGFAGFVLANGSMSSNQSGEGDIRKAIVEADLVDC
                     MVALPGQLFYSTQIPVCLWFIAKNKNDGKRRDRRKQTLFIDARKMGTMVDRVHRELTE
                     ADIKRIVDTYHAWRGEKSAGTYDDVAGYCKSSTLADIASHGHVLTPGRYVGAEEVEDD
                     GEPFDEKMKRLVAELESQFAESAKLEGAIKENLKRLGYGK"
     gene            1390..1812
                     /locus_tag="PKO06_10500"
     CDS             1390..1812
                     /locus_tag="PKO06_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNV70118.1"
                     /translation="MIDCTKFQKSLKHLERQLENYLRAKSRPELTPLDREAIAESVIQ
                     RFEVCYDTLWKDLKRYLIEELGLSEVPNSPKPILKLAGQNNLFVSSVEKWLEYANART
                     ATAHDYSEEKAAEALKVMNDFVADAVKLYETISGETWK"
     gene            1809..2186
                     /locus_tag="PKO06_10505"
     CDS             1809..2186
                     /locus_tag="PKO06_10505"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV70119.1"
                     /translation="MNRSLDVTKKQGEILAALLKRFLPGTAVWAYGSRVKGTARPYSD
                     LDLVVFATPEQRSAVADLKEALDESDLPFIVDLHVWGEIPARFHEMIRQAYVILAEGQ
                     TPSNDEKTTKPPLHGATTPASER"
     gene            2214..2636
                     /locus_tag="PKO06_10510"
     CDS             2214..2636
                     /locus_tag="PKO06_10510"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HI0074 family nucleotidyltransferase
                     substrate-binding subunit"
                     /protein_id="HNV70120.1"
                     /translation="MPLELDSLKKAVAALQAVLAKSQDTSLMSSLDDITQNAIRSGVI
                     QHFEFTYELCWKFMKRWLETNISPTTADGVTRRELFRLAAEQRLITDVEEWMKHHEAH
                     GRISQTYEPEIATHVYSSVSAFARDAKYFLQTLDARND"
     gene            2629..>3271
                     /locus_tag="PKO06_10515"
     CDS             2629..>3271
                     /locus_tag="PKO06_10515"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV70121.1"
                     /translation="MIDVSPAHLETIQRILSEHVPTCEVRAFGSRVTWTAKDYSDLDL
                     AIVGTKRLDVGVMRRLKEAFEESDLPFRVDVIDWYAISDSFRKVIKKKCVVFEQSGKV
                     TDDSSLSRNSKKLVDLLLTSKDGDWGKDSSAQGFVPFRVIRGTDFPRVRRGDLSSVPL
                     RFLSEKTVCRRTLNSWDILIETAGGTKDQPTGRTLLIKPRTLEGFTTPVTCASL"
BASE COUNT          777 a          900 c          922 g          672 t
ORIGIN      
        1 tgccgaagga agcccgctgg tcgcacttgc aggcgaacgc aaaacagccg tcgatcggca
       61 agctggtcga tgatgccatg gtcgccctcg aacgcgacaa tccccgcctc aagggcgttc
      121 tgccgaaaga ctatgcccgc cccgggctcg acaagcaccg actgggcgag ctgatcgacc
      181 tgatcggcac catcggcctg ggtgacgccg cgaaccgttc gaaagacatt ctgggccggg
      241 tgtatgagta ttttctgacc cagttcgctt cggcagaagg caagaacggg gggcagttct
      301 atacacccgg ttgcattgtc aggctgttgg tcggcatgct cagcccgtat aaaggccgcg
      361 tctatgaccc gtgctgtggc tcgggtggca tgtttgtcca gtctgagaag ttcgttctcg
      421 agcatggcgg gcgcattggc gacatcagca tctacggtca ggaaagcaac gcgaccacgc
      481 ggcgactggc gatcatgaat ctggcgattc gcggcatcga ggcggatttc ggccccgagc
      541 acgccgatac gtttcggcgc gatctgcaca aagatctcaa ggcccagttc gtgctggcca
      601 atccgccgtt caacgattcc gactggcatc gcgtcgacga cgatgtgcgc tggcagttcg
      661 gggtgccgcc caagggcaac gccaactacg cctgggttca gcacttcatt catcacctgg
      721 cgcccgatgg tttcgccggg ttcgtgctgg cgaacggcag tatgtcttcg aaccagtccg
      781 gtgaaggcga tatccgcaaa gcgattgttg aagccgatct cgtcgactgc atggtggcac
      841 tcccgggcca gttgttctac tcgacccaga ttccggtctg tctctggttc atcgccaaga
      901 acaagaacga cggcaaacgc cgtgaccggc gcaagcagac gctgttcatc gacgcccgca
      961 agatgggcac gatggtcgac agggtccacc gggaactgac cgaagccgat atcaagcgta
     1021 ttgtcgacac ctaccacgcc tggcgcggcg agaagagtgc cggcacctac gatgatgttg
     1081 ccggatactg taaatcatca actctggccg atatcgcgtc acacgggcat gtactcacgc
     1141 cgggccggta tgtcggcgcc gaagaagtcg aagatgacgg cgaacccttc gacgagaaga
     1201 tgaagcgcct ggtcgccgag cttgagtccc agtttgccga atcagccaag ctcgaaggcg
     1261 ccatcaaaga gaacctcaaa aggctgggct atggcaaata gtagggacac cgccgaattg
     1321 cgtgaacagg cgtcgcagag gtgatatcgt gaaagagaca gcaagtgcaa tggatggaga
     1381 acggatcgta tgattgattg tacaaagttt cagaaatcgc tgaaacacct cgaacggcaa
     1441 ctggagaatt atctgcgggc caaatcccgg cctgagctga cgccgctcga ccgtgaagcg
     1501 atcgccgaat cggtgattca gcgattcgaa gtctgctatg acacgctctg gaaggacctg
     1561 aaacgctatc tgatcgaaga actgggactt tccgaggttc ccaacagccc caagcccatt
     1621 ctgaagctgg cagggcagaa caacctgttt gtctcttccg tcgaaaagtg gctcgagtat
     1681 gccaatgcgc gaaccgcaac cgcccatgac tacagcgagg aaaaggccgc tgaagctctg
     1741 aaggttatga acgacttcgt cgctgatgcc gtgaaattgt acgagacgat ctcaggtgaa
     1801 acgtggaaat gaaccgttcg ttggatgtga caaagaagca gggcgagatt ctggcggccc
     1861 tgctgaaacg gtttctgccg ggaacggcgg tctgggcgta tggttcacgc gtgaaaggca
     1921 ccgctcgtcc gtattctgat cttgatctgg tggtgttcgc gacccccgaa cagcgttccg
     1981 cggttgccga cctgaaagag gcgctcgatg agagcgacct cccgttcatc gttgatcttc
     2041 atgtctgggg tgagattccg gcccgttttc acgagatgat ccgtcaagcc tatgtcattc
     2101 ttgcggaagg ccagacccca tcaaacgatg agaaaacaac aaagcctccg cttcacggag
     2161 caacgacgcc ggcgagtgag cggtgacagg aatgcgagtg ttggagatga gtcatgcctc
     2221 ttgaactcga cagcttgaaa aaggccgtgg ccgcgttgca ggccgtattg gcgaagagcc
     2281 aggacacatc actcatgagc agtctggacg acatcaccca aaacgccatc agatcgggtg
     2341 tcattcagca tttcgagttc acctatgagc tgtgctggaa gttcatgaaa cgatggctcg
     2401 aaacgaacat cagcccgacc accgccgacg gcgtgacccg ccgcgaactg tttcgactgg
     2461 ccgctgaaca acgcttgatt actgacgttg aagagtggat gaaacaccac gaagctcatg
     2521 gtcgcatcag ccaaacgtac gagcccgaaa tcgccacgca cgtttacagt tcagtctctg
     2581 cctttgctcg cgatgcgaag tattttctgc agaccttgga tgcccggaat gattgacgtc
     2641 agccctgctc acctcgaaac gattcagcgc attctgtctg aacacgtccc aacctgcgag
     2701 gtgcgcgcct tcggctctcg agtaacctgg acagccaaag actactccga cctcgacctc
     2761 gccatcgtcg gaaccaaacg cctcgatgtt ggtgtcatgc gccgcctgaa agaagccttc
     2821 gaagagtccg acctcccctt ccgcgtcgac gtcatcgact ggtacgctat ctctgattcc
     2881 ttccgcaagg ttattaaaaa aaagtgtgtc gtctttgaac aatcaggaaa agtgactgat
     2941 gatagtagtt tgtccagaaa ctctaagaaa ctggtagatc ttttactgac atcaaaagat
     3001 ggagactggg gcaaggattc ctcagcacaa gggtttgttc catttcgtgt aatcagagga
     3061 acggactttc caagagttcg acgtggggac ttgtcttccg tgccacttcg ctttttgtca
     3121 gagaaaactg tctgtcgtag aactttaaat tcttgggaca tcttgatcga aacagcggga
     3181 ggaacgaaag atcagcctac gggcagaact ttactaataa aacctcgcac tctcgaaggt
     3241 ttcacaacac cagtgacgtg cgcaagcttg c
//
LOCUS       DAORHD010000463         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33343_length_3260_cov_3.264275, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000463 DAORHD010000000
VERSION     DAORHD010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33343_length_3260_cov_3.264275"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PKO06_10520"
     CDS             complement(<1..778)
                     /locus_tag="PKO06_10520"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HNV70122.1"
                     /translation="MTATALLFAFFTGLCLSACTGFRAFVPPLILGLTHRLLPEYVPL
                     GASAAYLSNTLVLIALGMAVIIEFLGDKIPWVDHALDVVQVPVKMGFAGLLVAFLVPP
                     EHASWMLTLIAAAIGSGTALTVHSAKASVRVGSTATTGGILNPVISFVEEIAAVVGTI
                     VSIFLWPLAILAFFWLMWRVFRFLFGRTGGNEGTIAKAPPSEFWYRLARFGTWVYAKI
                     YHRLQIAGTEHLPQQGPFVVVGNHASMLDGSRPEWESAVAD"
     gene            complement(866..2338)
                     /locus_tag="PKO06_10525"
     CDS             complement(866..2338)
                     /locus_tag="PKO06_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNV70123.1"
                     /translation="MIRFGYIVSVVGLVMQGFAALMAVPLLLAIAYGEKQTFLAFAAC
                     FVVSASIGRLAMRCHPDSNFDDLGRMEGMGVVTFGWLAVAICGAIPYLFFKLGPIDAL
                     FESMSGFTTTGATTFQDFALYPPALFFYRGYTQWLGGVGILVLFVAVLPHLAVSGRQL
                     FFAETSAASKDKLTPRISDTARRLWGWYFLLTLLQTVLMGFYGMPWNDSFANAFATMA
                     AGGFSPHPQSIMGYANPAVEWIIILFMFLAGANFTLQVRALRGNLRALWDNAELRFYL
                     VIITWSSAILTLILARSTPLAFTIDALRAALFQNLSILTTTGFATHDFNLWSDSAKTV
                     LLVLMFIGGCSGSAGGGIKVIRLLLLLRYIGRAIIRAIHPKAIITIKLGKRVFSESEV
                     QPILSFIFLYFCFFAVGGTIAALIENNLIVGYTGAIATLGNIGPGFGLIGPMGSFGTL
                     HPVTKLLYTVLMWAGRLEIIALVVFMSRETWSNMRFCRRD"
     gene            complement(2348..>3260)
                     /locus_tag="PKO06_10530"
     CDS             complement(2348..>3260)
                     /locus_tag="PKO06_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNV70124.1"
                     /translation="EYRLKPTDPGVGCALKDISLPKETLVTAIIRQEQVIIPSGATVL
                     ETGDKVIFMGLEAGMRKLEHRYNPHMGGRLDVIIVGGGNVGFMLAELLAPLSLVKLKI
                     VEKSPERCKFLAANLPDRVLVLHADGTDVEFLTSLQIQNCHCLVASTGTDERNLLVSL
                     LAKQLKVPNVITQTTKPGNVGFFEKVGVDVALSAHYNAIKNVVRKIDSEGIDVLTIIE
                     KGQADIVEVVVPSDFQPRRLMDLHLPTGVIIAAIRRSSHTIVPHGSDKIKPKDHLRVF
                     CTSGRGETLAEYLTETRHQAPAGGGEH"
BASE COUNT          795 a          902 c          956 g          607 t
ORIGIN      
        1 catcggcgac cgccgattcc cactcgggac gcgagccatc gagcatgctg gcgtggttcc
       61 cgacgacgac aaagggtccc tgttgcggca gatgctcggt tcccgcaatc tgcaggcgat
      121 ggtagatctt ggcatacacc caggttccga agcgggccag acgataccag aactcggacg
      181 gcggcgcttt ggcgatcgtg ccttcgttgc cgccggtacg accgaacaga aagcgaaaga
      241 cccgccacat cagccagaag aaggccagaa tggcgagagg ccagagaaag atcgagacga
      301 tcgtgccgac gacggcagcg atctcttcga caaagctgat gaccgggttc aggattccgc
      361 ctgtggttgc ggttgaaccg actcgtacgg aagccttggc cgagtgaacg gtgagcgcgg
      421 tgccgctgcc gatcgcggcg gcgatcagcg tcagcatcca gctcgcgtgc tccggtggca
      481 cgagaaaagc gacgagcaga ccggcgaacc ccattttgac aggcacctga acgacatcaa
      541 gcgcatgatc gacccagggg atcttgtcgc cgagaaactc gataatgacg gccatgccaa
      601 gagcgatgag caccagcgtg tttgacagat aggcggcaga agcgcccaga ggaacatact
      661 cgggcagcag acgatgcgtg agtcccagaa tcagcggcgg aacaaaggcc cgaaacccgg
      721 tgcaggccga gagacacaaa ccggtgaaaa aggcgaacaa cagagccgtc gcggtcattc
      781 tgtcacctct gaaaagactg gtcagaaact gcctttaccc tacaacagat catctgctga
      841 ggcaaccttc tcggaccgga gcgaatcagt ctcgcctgca gaaacgcatg tttgaccagg
      901 tttcgcggct cataaagacg acaagagcga tgatttcgag tcggcccgcc cacatgagaa
      961 cggtgtatag cagcttggtg acagggtgca aggtcccgaa agagcccatg gggccgatga
     1021 gcccgaatcc agggccgatg ttcccgagcg tggcgattgc tcccgtgtac ccgacgatga
     1081 gattgttctc gatcagcgcg gcaatggttc ccccgacagc gaagaaacag aagtacagga
     1141 aaatgaacga gagaatcggt tgaacttctg attctgagaa cacgcgcttc cccagtttga
     1201 tggtgatgat ggctttggga tgaatggctc gaatgatcgc gcgcccgata tagcgcagca
     1261 acaacagcag gcgaatcacc ttgatgccgc cgccggctga tcctgaacag ccgccgatga
     1321 acatcagtac cagcagaacc gtcttcgcgc tgtcgctcca gaggttaaaa tcgtgcgtcg
     1381 cgaatccggt ggttgtcaga atactgaggt tttgaaacag tgcggctctc agcgcgtcga
     1441 tcgtgaaagc aagcggagtg ctgcgtgcca gaatgagcgt gagtatcgca ctcgaccacg
     1501 tgatgatgac caggtagaat cgcagctcgg cgttgtccca gagtgcacgc aggttcccac
     1561 gcagagcacg aacctgtaag gtgaagttcg ctccggcaag aaacatgaag agaatgatga
     1621 tccattcgac ggcgggattg gcgtacccca tgatgctctg cggatgcgga gagaagccgc
     1681 ccgcggccat ggttgcgaag gcattcgcga acgagtcgtt ccagggcatg ccataaaagc
     1741 ccatgagaac ggtctgaaga agggtgagca gaaaatacca accccacaaa cgccgcgcgg
     1801 tatcgctgat gcgcggggta agcttgtctt tcgaagccgc cgaggtttct gcgaaaaaca
     1861 actggcggcc tgaaaccgcg agatgcggca gaaccgccac aaacagaacc agaatgccga
     1921 ctcctccgag ccattgcgta taaccacggt agaaaaacaa agccggcgga tacagggcaa
     1981 aatcctgaaa ggtggtcgcc ccggttgtgg tgaagccact catcgattcg aacaacgcgt
     2041 cgatcgggcc aagtttgaaa aacagatagg gaatcgcacc gcagatcgcg acagccaacc
     2101 agccgaaagt gacaacgccc attccctcca tgcgtccgag gtcatcaaag ttggaatccg
     2161 gatggcaacg catggcaagc cttccgattg aagctgagac gacgaaacag gcggcgaagg
     2221 caagaaaggt ctgtttctcg ccatacgcaa tggctaacag caatggaacc gccatgaggg
     2281 cggcaaatcc ctgcatgacc aggcccacga ccgagacaat gtacccaaaa cggatcatat
     2341 catcgcttca gtgttcaccg ccgccggcag gtgcctgatg tctggtttcc gtgagatact
     2401 cggctaatgt ctcgccacga ccactggtac agaaaacccg cagatggtct ttgggcttga
     2461 tcttatcgct gccgtgcggc acgatggtgt gtgagctgcg acggatggcg gcgatgatca
     2521 cgcccgtggg caggtgtaga tccatcaatc gccggggctg aaagtctgag ggaaccacca
     2581 cttcgacgat gtctgcctgt cccttttcga taatggtcag aacatcgatt ccctcactgt
     2641 cgattttgcg cacgacgttc ttgatggcgt tgtaatgcgc actcaaagcg acatcgacgc
     2701 cgactttctc gaaaaagccg acatttcccg gtttcgtggt ttgcgtgatg acgttgggaa
     2761 ctttgagttg tttggcgagc agagaaacca gaaggttgcg ttcgtccgtg ccggttgatg
     2821 ccacgaggca gtgacagttc tgaatctgaa gtgacgtgag aaactcgacg tccgttccgt
     2881 ccgcgtgcaa gaccagaact cggtcgggaa gattggccgc cagaaacttg catcgctcgg
     2941 gtgacttctc aacgatcttg agtttcacca ggctcagcgg tgccagcagc tcggccagca
     3001 tgaagccgac gttgccgcca ccgacgatga tgacgtcgag acgtccgccc atgtgtggat
     3061 tgtagcgatg ttccagcttg cgcattcccg cctcgaggcc catgaaaatc accttgtcac
     3121 ccgtctccag aacggtggct ccgcttggaa tgatgacctg ttcctgcctg atgatcgcgg
     3181 ttaccagggt ttctttcggg agactgatgt cttttaacgc gcagccaaca ccaggatcag
     3241 tcggtttcaa cctgtactcc
//
LOCUS       DAORHD010000464         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33556_length_3247_cov_3.720551, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000464 DAORHD010000000
VERSION     DAORHD010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33556_length_3247_cov_3.720551"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..922)
                     /locus_tag="PKO06_10535"
     CDS             complement(116..922)
                     /locus_tag="PKO06_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNV70125.1"
                     /translation="MNREYHVIPSQTLGHPLEMLIFGHSGKPVLVFPSSEGRFFDYEN
                     FGMIGTIAPFIDSGKIQVFCIDGIDSESWYAPVPPADKARRANDYDYAIIHEVIPHIR
                     QHTVPHAGIMTHGVSFGAYHAANFYLRHPDVFDSCIALSGNYSIAFAVGAFCNSDVYF
                     NDPLMYLPNLHEDKILSQLREDLLLICCGQGPWEDWLGEARAVSHHLHTKNVPHVLDL
                     WGYDVGHDWPWWKKQILHFLGKLERAGCLRSDHRFHREQVESFVREFHHL"
     gene            complement(919..2079)
                     /locus_tag="PKO06_10540"
     CDS             complement(919..2079)
                     /locus_tag="PKO06_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HNV70126.1"
                     /translation="MRLVYLSPHYPRHFSQFPQSLARYGVQVLGVSDQPDECLEPSLR
                     SALAGHYRVDGLENREQVLTACHFFREHFGPFDRVESHLEPWLEMEGVIREVFQVTGP
                     KPADLEYLKRKSLMKKVFQDARVPVARGTIVETIEQCRSFIERRYHVFIKPDIGVGAS
                     DTYTIRSDDDLNYFFNNRSSYSYFLEEYLSGVIESFDGLTDREGNVVFCTSHVFSNDI
                     HQVVKKNENLWYYSQREIPIDLHEAGKRVVKAANIREKFFHIEFFRVADGSLKALEIN
                     LRPPGGLTTHMFNFACDIDVYDWWARIIAGQSPQQSFDRRYHCAFVSRKHDRGYRHSV
                     PDLRSRFGSGIVLEQPMNPIEFAVMGNVGFLVRSPDLVHLREMISSIIEEAA"
     gene            complement(2139..2759)
                     /locus_tag="PKO06_10545"
     CDS             complement(2139..2759)
                     /locus_tag="PKO06_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70127.1"
                     /translation="MKPLSVILLLSFTLVSPLWAQVPPTDTILASLSLAIREPKPETL
                     LIEKVRRGEEIFLQARSGSAAPFWTSDCLGSEDKKFLFDGKPVTLISCDLDSDKHPEL
                     LTAAFYGPMASGLYGFIYQPTTRTFSVISCKYPNPDDNRDLLVSDVHEEDGSDFIITS
                     DGKIRVLGIIYPTTEGQEPKPGTFHFSFQKGAFHAGSIEPLSEPDK"
BASE COUNT          727 a          836 c          975 g          709 t
ORIGIN      
        1 tcccccggat tgttctctcg ccccatgcta gtggaaccct cgcaaaaggg caagatcaaa
       61 acgcaaaacg gctgccaacg aggcgaaggc agccgttttg cgagggaacg aatgcttaca
      121 ggtgatggaa ctcccggacg aaggactcca cctgttcgcg gtgaaaacgg tggtctgaac
      181 gcaggcaacc cgctcgttcg agtttgccga ggaagtggag aatctgtttc ttccaccacg
      241 gccagtcatg gccgacatcg tatccccaca gatcaagaac gtgaggaaca ttcttggtgt
      301 gcaggtggtg tgatacggcg cgagcctctc cgagccagtc ttcccatggc ccctgcccgc
      361 agcagatgag cagcagatct tcgcgcaact ggctcagtat cttgtcttcg tgaaggttgg
      421 gcaggtacat cagtgggtcg ttgaaataca cgtcgctgtt gcagaacgcg ccgaccgcaa
      481 acgcgatact gtagttgccg ctcagagcga tgcaggagtc gaagacatcc ggatgcctca
      541 ggtagaagtt ggcggcatga tatgcgccga aactgacgcc gtgagtcatg attccggcgt
      601 ggggaacggt gtgctgacga atgtgtggaa tgacctcgtg gatgatcgcg tagtcatagt
      661 cgtttgcccg tctggctttg tcggccgggg gcaccggcgc ataccagctt tcgctgtcga
      721 tgccgtcgat gcagaatacc tgaatctttc ccgaatcgat gaagggggcg atcgtaccaa
      781 tcatgccgaa gttctcgtaa tcgaagaaac ggccttcaga agaagggaac acgagaaccg
      841 gcttgccgga atgcccgaaa atcaacatct cgagcgggtg cccgagggtc tggctgggaa
      901 tgacgtgata ttctcgattc atgcggcctc ctcgatgata ctggagatca tttccctgag
      961 atgaacgagg tcgggtgaac gcaccagaaa accgacgttg cccatgacgg caaactcgat
     1021 cggattcatg ggttgttcga gaacgattcc ggaaccgaac cgactgcgca gatcaggaac
     1081 gctgtgccga taaccgcgat catgctttcg tgagacgaag gcgcagtgat accgacggtc
     1141 gaacgactgt tggggggact gtccggcgat gatgcgagcc caccagtcgt agacgtcgat
     1201 gtcgcaggcg aagttgaaca tgtgcgtcgt cagaccgccc ggcgggcgca ggttgatctc
     1261 gagcgctttg agcgaaccat cggccacgcg gaaaaactcg atatggaaga acttttcgcg
     1321 aatgttcgcg gccttgacca ctcgcttgcc ggcctcgtgc aggtcgatcg ggatctcgcg
     1381 ctgcgagtaa taccacaggt tctcgttctt cttgaccacc tggtgaatgt cattgctgaa
     1441 tacatgactg gtacagaaga caacgtttcc ctcgcggtcg gtcaacccgt cgaaagactc
     1501 gatgacgccg ctcagatact cctccagaaa gtaggagtac gagctgcgat tgttgaagaa
     1561 ataattcaga tcatcgtcag agcgaattgt ataagtgtcc gaagctccga ctccaatgtc
     1621 gggtttgatg aacacgtggt atcgcctctc gatgaaagat cggcactgtt cgatggtttc
     1681 gacgatcgtt ccccgtgcta cggggacccg ggcatcctga aaaaccttct tcatcagtga
     1741 cttgcgtttc agatattcga gatcggcggg tttggggccg gtgacctgaa atacctcacg
     1801 aatgacgcct tccatttcca gccagggttc gagatgtgat tccacccgat cgaacgggcc
     1861 gaagtgttcc cggaaaaagt ggcaggcggt cagaacctgt tcgcgattct cgagaccatc
     1921 gacacgatag tgcccggcaa gtgccgatct gagcgaaggt tccagacatt cgtccggttg
     1981 atcagaaaca ccgagaacct gaacgccgta acgggccaga gactgcggga actgggaaaa
     2041 atgcctcgga taatggggtg acagatacac gagtcgcacg tttcaattct ccagagagat
     2101 gaagttatcc ggcgactcga ttatacctga aaatgtgctt acttgtcggg ttcgctgaga
     2161 ggctcgatgg aaccagcgtg gaaggccccc ttctggaagc tgaagtggaa cgtaccgggc
     2221 ttgggctcct gcccttcggt cgtcggataa atgattccca ggacccgaat cttgccgtcc
     2281 gaagtgatga tgaagtctga accgtcttcc tcgtggacat ccgagaccag tagatcccgg
     2341 ttgtcatcgg ggttgggata cttgcaggag atgacgctga atgtccgggt cgttggctga
     2401 tagatgaaac cgtacaagcc gctggccatg gggccgtaga acgccgcggt cagcaattct
     2461 ggatgtttgt ccgagtcgag atcgcaggaa atcagcgtca ctggcttgcc atcgaacaga
     2521 aacttcttat cctcgctgcc gaggcagtcg ctggtccaga atggggcggc acttccgctg
     2581 cgtgcctgca gaaaaatctc ttcgccgcgc cgaactttct cgatcagcag ggtttccggt
     2641 ttcggttccc ggattgccag cgacagggaa gccaggatgg tatcagtcgg aggaacctga
     2701 gcccagagcg gactgacaag tgtgaatgac agcagaagaa tcacagacaa aggtttcatg
     2761 gtgcagctcc tgttcaacga tcccattgat agaggttgta cgatttcatg atgctgatca
     2821 acgtgcgggc gtaattcggg tcggtggcgt agcccgcccg atgtacttca cggacgaatt
     2881 gtgacacatc attttgggga aacgcaagag tgagtggcag ttgccggggt cagtccattc
     2941 tgacccgttt ttcccctggc gtgagcgaat cgttgagaaa gccaaaggga aaacccaagc
     3001 gagagattga aatgggctgg ggcttttgcc tgtttcaaga ggtttggggt agcgggctcc
     3061 tgccgtttgt ggtacaggag aggcatgcgc cgtcgtatgc gtttcctcct gatcctgggt
     3121 cttcttgcgc tcgttgtcac ttgcgcggga tgcggctggt tgtttggcac gaccgacggc
     3181 gccttgtgcc tcctgggatt cctcggaaga acgcagacgt cggtcgccga tctgcgattc
     3241 gcgggag
//
LOCUS       DAORHD010000465         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33563_length_3247_cov_3.015664, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000465 DAORHD010000000
VERSION     DAORHD010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33563_length_3247_cov_3.015664"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PKO06_10550"
     CDS             complement(<1..1016)
                     /locus_tag="PKO06_10550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNV70128.1"
                     /translation="MAQLEHIEAIEKRLWSAADTLRANSNYAGNEYFLPVMGLVFLRH
                     AYSRFLSVKDVIEAALPRRGGKTRPLTKEDFSQKSSIFLQPQAQFDTLVALPDSADRA
                     RAIIDAMEFIEADYESLRGVLPKSEYQELDNGVLGQLLRTLNPEELKRVSGDVFGRIY
                     EYFLTQFADQKAHDGGEFFTPVSLVSLIANVIEPGRGKVLDPACGSGGMFVQSARVVE
                     RRHENPAEKLTFYGLEKNATTIRLAKMNLAVHGLEGNIQKAITYYEDPHELLRKSDFV
                     MANPPFNVDEIDADKVKSDPRLPFGLPGINKKGKVSNGNYVWISYFYSYLNERGRAGF
                     VMSS"
     gene            1290..1736
                     /locus_tag="PKO06_10555"
     CDS             1290..1736
                     /locus_tag="PKO06_10555"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV70129.1"
                     /translation="MKPSEQLKAEHEAILVMLAVLEKICSRLERRETIPESHLAGILE
                     FFQVFADKCHHAKEEEFLFPAYQSVGIPREGGPIGCMLAEHQEGRQAIAGMKAGLEVL
                     TREGPGTFIAHARAYASLLSEHIQKENNVLFRWVIPFCPLQNRPRF"
     gene            1885..2532
                     /locus_tag="PKO06_10560"
     CDS             1885..2532
                     /locus_tag="PKO06_10560"
                     /inference="COORDINATES: protein motif:HMM:NF039786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HNV70130.1"
                     /translation="MFSNRIVLAQLSIVLFLGVFGPTMTMLSAHCDTFAGPVLIEAQQ
                     ALEGGEVRALLKWVPPEREEQIKRAFAKALRQRTTDRQKADSAFFETFVRIHREGEGA
                     AFTGIKPADTRLPPAITAADRAVETGSAAALFDLLSRNLTQSVQAKFARVIARSKTKN
                     KSVAAGREYVAAYVEYVHYVEAVSNLLNADDQHHRLTETKRTGQETCAPCSGHGK"
     gene            2562..3155
                     /locus_tag="PKO06_10565"
     CDS             2562..3155
                     /locus_tag="PKO06_10565"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV70131.1"
                     /translation="MPRATNAEQDRKTQILLAARNVVFSEGTSKLTMRRVGEAVDISE
                     PAVYRHFKNKEDLLRALINFMFEGWEEKLVALKGENISASEKLIKLGKIHLTHLIDHQ
                     FNPVLLLSEVGLTEQPSITEALNEKGERIAAVMASILKEGCASGEFAADLNIKSAILA
                     ILGVLQGSLIRWTLSRSTRGLASDLEGALRLIIKGLA"
BASE COUNT          705 a          888 c          971 g          683 t
ORIGIN      
        1 tgcgacgaca tgacgaaccc ggcccggccc cgctcgttca ggtagctgta gaaatagctg
       61 atccagacgt agttgccgtt cgagaccttg cctttcttgt tgatgcccgg caacccgaac
      121 ggcaggcgcg ggtcgctctt gaccttgtcg gcgtcgattt cgtcaacatt gaagggcgga
      181 ttggccatga cgaagtcgga ctttcgcaac agctcgtggg ggtcttcgta gtaggtgatc
      241 gccttctgga tgttcccttc gaggccatgc accgccaggt tcatcttggc cagtcgaatg
      301 gtggtcgcat ttttttcgag accgtagaag gtgagctttt cagccgggtt ttcgtggcgg
      361 cgctcgacca cgcgggcgct ctgcacgaac ataccgcccg agccgcaggc cgggtcgagc
      421 actttgccgc ggccgggttc gatgacgttg gcgatcagcg agaccagcga caccggggtg
      481 aagaactcgc cgccgtcgtg tgctttctgg tctgcgaact gggtgagaaa atactcatag
      541 atgcggccga acacatcgcc ggagacccgc ttgagttctt ccgggttgag cgtgcgcaga
      601 agctggccca gcacgccgtt gtcgagttcc tgatattcac tcttcggcaa aaccccgcgc
      661 agactctcgt agtcggcttc gatgaattcc atcgcgtcga tgatggcccg ggcgcgatcg
      721 gcgctgtcgg gcaaggcgac gagcgtatcg aactgtgcct gaggctgcag aaagatggaa
      781 ctcttctgcg aaaagtcttc tttggtcagc ggccgagtct taccgccgcg ccggggcagg
      841 gctgcctcga tgacatcttt gaccgacaga aagcgactgt aggcatggcg cagaaacacc
      901 aggcccatga ccggcagaaa gtactcgttg ccggcatagt tcgagttggc gcgcagggta
      961 tccgcggcgc tccagagacg cttttcaatg gcttcgatgt gttcgagctg ggccacgtga
     1021 ctcactctcc cttgctgatc tgtttcagaa tgacaccaaa gtgtatcatg ttctccggtg
     1081 tttgtaagtg cctcaaagcg ggcatcaccc ttcctttctg tctcaatctt cgaggggtgc
     1141 accatgttcc gcgggggtgt ggtgtgggcc tgagccggat tggctttcct tgccgagaca
     1201 aatgatacca aaatactgtt tgacaaacgc gcatgttcga gtaactttaa aagtagttat
     1261 cgataactaa ctctcaacag gaggcgcgta tgaaaccgtc tgaacaactc aaggccgaac
     1321 atgaggcaat tctggtcatg ctggctgtcc tcgaaaagat ctgcagcaga ctggaacggc
     1381 gcgaaacaat tccagagagc catctggcgg gcattctgga gttctttcag gtgtttgcgg
     1441 acaagtgtca tcatgcgaag gaagaagagt tcctgttccc cgcgtatcag tctgttggca
     1501 taccccggga gggcgggccc attgggtgca tgctggccga acatcaggaa ggacggcagg
     1561 ctatcgctgg catgaaggcg gggttagagg ttctgacaag ggaagggccc ggcacgttca
     1621 ttgcccacgc ccgcgcctac gcgagcctgc tctcagagca cattcagaaa gaaaacaatg
     1681 tcctgtttcg atgggtgatt ccgttttgcc cgctgcaaaa caggccgaga ttctgaaaga
     1741 gttcgaccat ctcgaggaac accgaatcgg tcacgggaga cacgaggaat tccaccgcct
     1801 gatcgaaact cttgaagcga cgtatctcaa cgcaacgaac taggacaaat gaaccctaca
     1861 ccatcaacca ccggaggcac tgcaatgttt tccaatcgta tcgtgttggc gcaactgtcg
     1921 atcgttctgt tcctgggtgt gttcgggccg acaatgacca tgttgtcggc gcattgtgac
     1981 accttcgccg ggccggtgct catcgaggct caacaggcgc ttgaaggcgg cgaggttcgc
     2041 gccctgctca agtgggtgcc gcctgaacgc gaagagcaga tcaagcgagc atttgcaaaa
     2101 gcgttgagac aacgaacgac ggaccggcag aaggccgact cagcgttctt tgagacgttc
     2161 gtgcgcatcc atcgcgaggg agaaggggcc gccttcaccg gaatcaaacc ggcggatacc
     2221 aggttgccgc cggccatcac cgcggcggac cgggccgtcg agaccggctc tgccgcggcg
     2281 ctgtttgacc tgctgtcacg aaatctcacg caaagcgtgc aggcgaagtt cgcccgggtg
     2341 atcgcccgaa gcaagactaa aaacaagagt gtggccgccg gccgtgaata cgttgccgcc
     2401 tatgtggagt atgtgcatta tgtcgaagcc gtcagcaatc ttctcaacgc tgacgaccag
     2461 catcatcgct tgaccgaaac caagaggacc gggcaggaaa cgtgtgctcc ctgctccggt
     2521 catggtaaat aggaagagaa cctttcacgg agggatactg tatgccgcgc gcaaccaatg
     2581 ccgagcaaga caggaagacg cagattctgc ttgctgcacg caacgtggtg ttttcagagg
     2641 gaacgtcgaa gctgaccatg cggcgcgtgg gtgaagcggt cgatatctct gaaccagccg
     2701 tgtatcgcca cttcaagaac aaggaagatc tgctgcgcgc gctgatcaac ttcatgttcg
     2761 agggctggga agagaaactg gtggcgctga agggcgaaaa catctccgct tcagagaaac
     2821 tgatcaaact cggcaagatt catctgacgc acctcatcga ccaccagttc aaccctgtgc
     2881 ttctcctttc cgaagtcggt ctgaccgaac agcccagcat tacggaagcg ctgaacgaga
     2941 aaggggagag aatcgccgcg gtcatggctt cgatcctgaa ggaagggtgc gcctcaggcg
     3001 aattcgctgc cgatctgaac atcaagtcgg cgattctggc gattctgggc gtgctacagg
     3061 gaagcctgat tcgctggact ctgtcgcgtt cgaccagagg tctggcgtcc gaccttgaag
     3121 gcgcgctgag gctgatcatc aaaggtcttg catagaatcg atcagggagt tcgccggtgc
     3181 gactctcgcg atcggggtgt tgaaccactg gtcggctgat tcgctgtatc ttccgttcgc
     3241 tcgtgct
//
LOCUS       DAORHD010000466         3245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33588_length_3245_cov_4.224765, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000466 DAORHD010000000
VERSION     DAORHD010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33588_length_3245_cov_4.224765"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1357)
                     /locus_tag="PKO06_10570"
     CDS             complement(83..1357)
                     /locus_tag="PKO06_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018477839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HNV70132.1"
                     /translation="MNRYSPGFSFRWLVAFLVLICLPVTGYAGKLLIPMDDEQVDHLR
                     AYGLAYWVLDKQSLDVDWLLNYRFGSFLCDDLPAIQARARELGVTVRSIGDDQVAQIM
                     ARIEEENMERVILEKAPKIGVYSPDYAEPWDDAVKLVLDYAQVPYENFYDKEVLDGKI
                     TKYDWVHLHHEDFSGQFGKFYSSFRFADWYRRQVTLAQLDAQKYGFKKVWQLKHAVAR
                     TIRKFVLDGGFLFAMCSACDSLDIGLSAGETDIVASELDGDPLDPMYESRLDFDQSLA
                     FSGFKLITDPMVYEFSDIDVSNNYMGDAGNFKLFDFSAKVDPIPTMLTQNHRNVVDGF
                     FGQTTSFRKSLLKPSVTIMGENNDGISVKYIRGDLGKGCWAFYGGHDPADVSHIVGEG
                     PTKLDLHKNSPGYRLILNNILFPAAKKKKRKT"
     gene            complement(1374..>3245)
                     /locus_tag="PKO06_10575"
     CDS             complement(1374..>3245)
                     /locus_tag="PKO06_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70133.1"
                     /translation="LLNTIESRSVRYRRVDYLEFASDCLLALRRDKEAADLLRDAPET
                     RQHPPLLAKLARLLLSRNDLEAARAAYEQLVTMNPDSREYRRALGHCLSQMKRPDEAL
                     TVWRGIFGQMGISQLEAYQFLLDTLIEHRLYEESLDVFQEARAALGNAGLFAEEKAGV
                     LMTLNRESEAIDEFLLALPAGGFKIDIFEKLYGYHGKKVDLEQKLRDLMSSGEAFYVA
                     RRALIELYFREARPEQVAKVVALAQGGLSDDMLFERIQQALSTQADGFVRQIIVSLIQ
                     QFHQSSLAWRLAPGLLELPDQTPTELQEAREIATTLAARPNPPDFLLRRRLLIALAIF
                     HLDTLSDPQEAVRWLSQAESMPSGGSRREAFQMHVLACRLHTLQGRFSDTDNDLARLR
                     GLIGDPTAPAATPPATDPFGDSGAEGGLDLGESDYSNLVGGELTADDRAQFIYLEAWV
                     LCHKGEYQPALTKLRSIIDEFPSSTWVNDALALALEITMGSIGTMEALGKLFGAERAV
                     AVGSFPAALDLYEQVRVLASGTSLSQEAEARHILLSERTASPAALIARIDAFRKANPA
                     QLTGADLLLLKHRLQRRMNSSADERQQTLREFLDRYPGDLRGRRVTRLLQSPAEASR"
BASE COUNT          682 a          915 c         1007 g          641 t
ORIGIN      
        1 aaagtaagcc agtaaaccgg caacctgtga ggtggcaaac ccgagggcta ccagcggaag
       61 aaaaatcgcg atcatgacga catcaggtct tgcgcttttt tttcttggcc gccggaaaga
      121 ggatgttgtt gaggatcaac cggtacccgg gactgttctt gtgcagatca agcttggtgg
      181 ggccttctcc gacgatgtga ctgacgtcgg cagggtcatg accgccgtaa aaggcccagc
      241 agcccttgcc gaggtcgccg cgaatgtact tgaccgagat cccgtcgttg ttctcgccca
      301 tgatggtgac cgacggcttg agcagcgatt ttcggaacga cgtggtctgg ccgaaaaaac
      361 catcgacgac gttgcgatga ttctgggtca acatggtcgg aatcggatcg accttggcgg
      421 aaaaatcgaa gagtttgaag ttcccggcat ctcccatgta gttgttggag acatcgatgt
      481 cggaaaactc atagaccatg ggatcggtga tcagcttgaa gccgctgaag gcaaggctct
      541 gatcgaaatc caacctggac tcatacatcg gatcgagggg atcgccatcg agttcgctgg
      601 cgacgatgtc ggtctcgccc gccgaaagcc cgatgtcgag cgagtcgcag gcagagcaca
      661 tcgcgaagag aaagccgccg tcgagcacga actttctgat ggtgcgggcg acagcatgtt
      721 tcagctgcca gactttcttg aacccgtatt tctgcgcatc gagttgcgcc agggtgacct
      781 gtcttctgta ccagtcggcg aaacgaaaac tcgagtagaa ttttccgaac tggccggaga
      841 agtcttcatg atgcaaatgc acccagtcat acttcgtgat cttcccgtct aggacttcct
      901 tgtcgtagaa gttttcatag ggcacctggg cgtaatcgag taccagtttc accgcgtcat
      961 cccacggttc ggcgtagtcc ggcgagtaga cgccgatctt gggagccttc tccagaatga
     1021 cccgctccat gttttcctct tcgatcctgg ccatgatctg cgcgacctgg tcgtcgccga
     1081 tggagcgcac ggtgaccccg agttcgcggg cccgggcctg aatcgccggc aggtcgtcgc
     1141 agaggaacga accgaagcga tagttgagca gccagtcgac atcgagcgat tgtttgtcga
     1201 gaacccagta ggcaagacca tacgcacgca gatgatcgac ctgctcgtca tccatgggaa
     1261 tgagcaattt cccggcatac ccggtgacgg gcaggcagat cagcaccaga aacgccacga
     1321 gccagcggaa ggaaaagccg ggtgaatatc gattcatgat gcctccggtc gtgtcaacga
     1381 gatgcttcgg ccgggctctg caacaggcgc gtgacacgcc gaccgcgcag gtcgcccggg
     1441 taccggtcga gaaattcgcg cagggtctgc tgacgctcat ctgcgctgga attcatgcgt
     1501 ctctgcagtc gatgcttgag taacagaaga tccgcaccgg tcagttgcgc ggggttggcc
     1561 ttgcgaaagg cgtcgatgcg tgcgatcagc gctgccggtg acgcggtgcg ctcagaaagc
     1621 agaatgtgac gcgcttccgc ttcctgagac aggcttgtac ccgaggcaag cacgcgcacc
     1681 tgctcgtaca ggtccagtgc tgccgggaag ctgccgaccg ccacggcgcg ctcggcaccg
     1741 aagagcttcc caagggcttc catggtaccg atcgagccca tggtgatctc gagtgccagg
     1801 gcgagggcat cgttgaccca ggttgacgag ggaaattcat cgataatgga gcgcagtttc
     1861 gtgagtgccg gctggtactc acccttgtgg cacagcaccc acgcttcaag gtagatgaat
     1921 tgggcgcggt cgtccgcggt cagttctccg cccacgaggt ttgaataatc ggactcacca
     1981 agatcgagtc ccccttcggc acccgagtcc ccgaagggat cggtcgccgg aggcgtggcc
     2041 gctggcgcgg tcgggtcgcc gatcaaacca cgcagacgtg ccagatcatt gtcggtatca
     2101 gaaaaacgcc cctgcagcgt gtgcagacgg caggccagaa cgtgcatctg aaaggcttcg
     2161 cggcggcttc ccccggacgg catggattct gcctgcgaca gccagcggac ggcttcctgg
     2221 ggatcggaaa gagtgtcgag gtgaaagatc gccagagcga tgagcagccg tcggcgcaac
     2281 agaaaatccg gagggttggg tctggcagca agcgtggtgg cgatttcccg ggcctcctgc
     2341 agttcggtcg gagtctggtc aggaagttcg agaagcccgg gagccaggcg ccaggccagc
     2401 gaagactgat gaaactgctg gatcagggag acgatgatct gacgcacgaa tccatcggcc
     2461 tgagtcgaaa gtgcctgctg aatgcgttcg aacagcatgt catcgctaag cccaccctgg
     2521 gctagcgcga cgaccttcgc gacttgctcg gggcgcgctt cgcggaaata caactcgatc
     2581 agcgcgcgtc gagcgacata gaatgcctcg ccgctcgaca tcagatcgcg aagcttctgt
     2641 tcgagatcga ccttcttgcc gtggtatccg tacaacttct cgaagatgtc gatcttgaat
     2701 ccaccggcgg gaagggccag aagaaattcg tcaatcgctt cgctctcgcg gttgagggtc
     2761 atcaggactc cggctttctc ttccgcgaac aaaccggcgt tgccgagggc ggcgcgcgct
     2821 tcctgaaaca cgtcgagaga ctcttcatag agacgatgct cgatcaaggt atcgagcaga
     2881 aactgatagg cctcgagctg tgagatcccc atctgtccga agatcccgcg ccagaccgtc
     2941 agggcttcgt cggggcgttt catctgactg aggcagtggc cgagagctcg tcggtattcg
     3001 cggctgtcgg ggttcatggt caccagttgc tcgtaggcgg ctcgcgcggc ttcgaggtcg
     3061 ttgcgactca acagcagtcg cgcgagtttg gccagcagcg ggggatgttg ccgagtttct
     3121 ggcgcgtcac gcagcaggtc cgcggcctct ttgtcgcgcc gcagagccag cagacagtca
     3181 ctggcgaatt cgagatagtc gacgcgtcga tagcgcacgg agcggctttc gatcgtattc
     3241 aacag
//
LOCUS       DAORHD010000467         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33631_length_3243_cov_2.630176, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000467 DAORHD010000000
VERSION     DAORHD010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33631_length_3243_cov_2.630176"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1117)
                     /locus_tag="PKO06_10580"
     CDS             complement(83..1117)
                     /locus_tag="PKO06_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV70134.1"
                     /translation="MKKNLSRFGLIICCATLALLAGSDIVAANSPMQTLQGFDPTSGQ
                     VTASSRSAAEGTPTGQVVMGTVHVRTRLNVRTSPWGPIIGKLYNDSQVRIIGREGDWF
                     KIDYNGQVAYVHSAYVTAPGMPSAAAPDGSAPSSSPTTSRPETTPAGEVPSGSLNAAD
                     FGYDSKYQPIFDLAEKYCQVNRKYTYAAGHMWKGYPAETDCSGFTCHLYQMLAQISGV
                     QPAFPKNQWYPNSDALRSNRNLATKITSAYPPPNPRDLIKPGDIFVLGHGSSGIGHVG
                     VFMGYTKSGNPVIAHSTPSTIGSQAIRGNRGCSGVRIEVMPSSYNSSRWTGIYRINGT
                     DQMLDKLAKK"
     gene            complement(1180..2913)
                     /locus_tag="PKO06_10585"
     CDS             complement(1180..2913)
                     /locus_tag="PKO06_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNV70135.1"
                     /translation="MIPLPEMLSTLVLALIGGGCGAIAARWFSAAARGVESGGLVVLG
                     AVTLTLPFASLIPGPEILGLVLVQGGLVILGRLYDEDRLPFAVLFPYLLLMLIIGALC
                     GLQVPAENRWVRLVLSLLWPLLVILCLKLASVIEEMPPLLCLGSGLTFLLFFPTQMAT
                     PPWAVVLTLVLVAASLVFLWRRYTGQSNLLGESGLYALGWLLAAVSMLGRSKSLLMFG
                     LLVPSMVVVFPFVLICMLILLSYVGNELYMLEAREESAYRWSLPRKRLVLLSGLVFLI
                     LNFLLLLWDAQAGPFGYLALGLLLAVSLVSFWRAFAGRERVRVPEIGSRISILGAPVN
                     VQTTSGVIDWLAARISEGQGFHHLVTADSLAVMRARRDDRFRAIMERASLAVADGAGL
                     IWAADFLGTPLPARVPGIGLVTEICRIAGTRNWKIYALGSRPGVVDKAIDVLRGRFPD
                     LKPVGTHHGFFREASAEEEAVMADIRAKAPDIILVALGVPRQEQFISRLRPLLTTGIA
                     IGVGGSFDVISGTLPRAPVLMQRFALEWLFRLYKEPKRLRRMLAIPAFVLAVLRDKMH
                     QADRKVSQKQV"
BASE COUNT          751 a          983 c          978 g          531 t
ORIGIN      
        1 cagagagcaa cagacaacgc cagagagaac aaccacgaaa cccgttgtcg aacacgaggg
       61 aaggtcatga gcatgagcat ctctacttct tcgccagttt atccagcatc tggtcggtac
      121 cattgatccg gtagatgcct gtccagcgcg agctgttgta tgaggaaggc atgacctcga
      181 tgcgcacgcc gctgcagccg cgattgccgc gaatcgcctg cgatccgatg gtcgacggtg
      241 tcgagtgcgc aatgacgggg ttccctgatt tcgtatagcc catgaacacg ccgacatgac
      301 cgatgccgct gctgccgtgc ccgagcacga agatgtcgcc aggcttgatc aggtctcgcg
      361 gattcggcgg gggataggcc gaggtgatct tggtcgccag gtttcgattc gagcgcagcg
      421 cgtcgctgtt cggataccat tgattctttg ggaacgccgg ctgaacgcca gatatctgcg
      481 ccagcatctg gtacaaatga caggtgaacc cggaacagtc ggtttccgcc ggatacccct
      541 tccacatgtg acccgcggca tacgtgtatt tgcggttgac ctggcagtat ttctcggcca
      601 gatcgaaaat cggctgatac ttcgagtcgt agccgaaatc agccgcgttc aaagagccag
      661 agggaacctc gcccgcgggt gtggtctcgg gccgggacgt cgtcggcgaa gaactgggcg
      721 ctgagccatc gggcgcggca gccgacggca ttccaggggc cgtcacatag gcggaatgga
      781 catacgccac ctggccgttg tagtcgatct tgaaccagtc gccctcgcgg ccgatgatgc
      841 gcacctggct gtcgttgtaa agtttgccga taatcgggcc ccaggggctg gtacgtacat
      901 tcaggcgggt gcgcacatga acggtaccca tgaccacctg accggtcggc gtgccttcgg
      961 cggctgaacg actcgaggcc gtcacctgcc ccgacgtcgg gtcgaagccc tgcaacgtct
     1021 gcatcgggct gttggcggcg acgatatcgg agccggccag cagggccaga gtcgcgcagc
     1081 agatgatcag accgaagcgg gaaagattct ttttcatgca aggacctcct gtactgcagg
     1141 tggtgaagac gcaatcacgg ttctctctct caaacgagat tatacctgtt tttgtgacac
     1201 cttcctgtct gcctgatgca ttttgtcacg cagaaccgcc agaacaaagg cgggaatcgc
     1261 cagcatccgc cgcagccgct tcggttcttt gtaaagacgg aacaaccact cgagcgcaaa
     1321 ccgctgcatc aggaccggtg cccgcggcag cgttcctgag atcacatcga agctcccgcc
     1381 gaccccgatg gcgattccgg tcgtcagcag cggacgcagg cgtgagatga actgttcctg
     1441 acgcggcaca cccagggcca cgagaatgat atcgggggct tttgcacgaa tatcagccat
     1501 gacggcctct tcctcggctg aggcttcgcg aaaaaaaccg tgatgagtgc ccaccggttt
     1561 caggtcggga aatcggccgc gtaacacgtc gatcgcctta tcgaccaccc cggggcgcga
     1621 tcccagcgca tagatcttcc agttccgggt gccggcgatg cgacatattt cggtcacgag
     1681 accaattccc ggcacgcgcg ccggcagcgg cgtgccaaga aagtcggcgg cccagatcag
     1741 cccggcacca tccgcaaccg ccagcgaagc ccgttccatg atcgcccgaa accggtcatc
     1801 gcgtcgggct cgcatgaccg ccagagaatc cgcggtcacc agatgatgga acccctgccc
     1861 ctcggaaatt cgtgccgcca gccaatcaat gactcccgag gttgtctgca cgttgaccgg
     1921 cgccccgaga atgctgatgc gactgccgat ctcgggcacc cgcacacgct cacgaccggc
     1981 gaacgctcgc cagaagctga ccagcgacac agccagcagc aacccgaggg cgagataccc
     2041 aaacggcccc gcctgggcgt cccacagcag caacagaaaa ttgaggatca gaaacaccaa
     2101 gccggacaag agtaccagtc gctttcgcgg caaagaccat cggtaggctg attcctcgcg
     2161 ggcttcgagc atgtagagct cgtttcccac ataggaaaga agaatcaaca tgcagatgag
     2221 cacgaacgga aaaaccacga ccatcgacgg caccagaaga ccgaacatca agagactctt
     2281 ggagcggccc agcatcgaaa ctgccgcgag cagccacccc aatgcgtaca agccggattc
     2341 gccaagaaga ttggattggc cggtataacg acgccagaga aaaacaagag aagcggcaac
     2401 gagtaccagc gtcagcacga cggcccaggg cggagtcgcc atctgggtcg ggaagaacag
     2461 cagaaaggtg agccccgagc cgagacacag gagggggggc atctcttcga tgaccgaggc
     2521 gagcttaagg cagagaatga ccagaagcgg ccacagcaga ctcagcacca ggcgaaccca
     2581 gcggttctcg gcaggcacct gcaacccgca cagagcgccg atgatcagca tcagcagaag
     2641 ataggggaac agcaccgcga atggcaggcg atcttcatca tagagtcggc cgagaatgac
     2701 caggccgccc tgcacgagga ccaacccgag aatttccggg ccgggaatca gagaggcgaa
     2761 gggtaacgtc aacgtcaccg cgcccaacac gacaagcccg ccgctttcga cgccccgggc
     2821 cgccgccgag aaccagcggg cggcgatcgc gccgcaaccg ccgccgatca aggccagcac
     2881 aagcgttgaa agcatttcgg gcaacgggat catcaaggta ttctagcgac caccgtactg
     2941 gcgcgcaagg ggcgatcggt acacacccga agaaacaacg tgaacaggaa aaaaccgggt
     3001 gaacaatacc gcggagtctg atatcctgag aaaagacagt cttgagtgca ggagtattcg
     3061 aaccatgggt atgtcttccc ggcggccaac ggtattgttc gtccttttca tcgcggcgac
     3121 tgcgtgcttg accctgttcg gttgcatggg tggcggcggt ggtggtagtg gggccacggc
     3181 gactgtcgat caggcggcca tcgccgcgac gctgaagagc ttcatgcggg cggccagtac
     3241 cgg
//
LOCUS       DAORHD010000468         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33697_length_3239_cov_2.720163, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000468 DAORHD010000000
VERSION     DAORHD010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33697_length_3239_cov_2.720163"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..532
                     /locus_tag="PKO06_10590"
                     /pseudo
     CDS             1..532
                     /locus_tag="PKO06_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            829..1263
                     /locus_tag="PKO06_10595"
     CDS             829..1263
                     /locus_tag="PKO06_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70136.1"
                     /translation="MTNEKLFRGIAGLFFLVLVVASSTPFFEFRVLDFSQPFHVCAAQ
                     RTRLLEAIRKFNQDFPPGISRLGPAELALLKSKGYLDASFGCPGDLVPRLGSGFLQAR
                     LEALVINLGINISTKVPVPGGEYTGVDLATTGRVTCSLHKVP"
     gene            1337..1777
                     /locus_tag="PKO06_10600"
     CDS             1337..1777
                     /locus_tag="PKO06_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70137.1"
                     /translation="MKLQEVVLGIGIFAVLVLMVLSYIPIFGWPYGRMIAREKACYAN
                     MRVLLGAIEMYNMDHSDFLDEMNPLVMAKLHTDGYLKSPMTPCPGDQAPRIGHPFLQA
                     RLAVLAANLGASQVAFRPIPGGTYAGTGLASDGRIVCTMHGTVE"
     gene            1814..2269
                     /locus_tag="PKO06_10605"
     CDS             1814..2269
                     /locus_tag="PKO06_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70138.1"
                     /translation="MTDQTKQGNQKTMQGFGVILVAVFILLFFVFGFGIRKARVHPRS
                     KTCYANLRVIIGAIEMYNMDHTRMMSTLGAPELAQLVSGKYLIVPVTCPGDSRPRFSN
                     TWIQGFFEQLSYRFGSFMILHTPVPAGSYQGAFLDTTGKPSCSLHGTVD"
     gene            complement(2331..>3239)
                     /locus_tag="PKO06_10610"
     CDS             complement(2331..>3239)
                     /locus_tag="PKO06_10610"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNV70139.1"
                     /translation="VQNGKWFPSVLDEAFTRLRPHIPAPYLEELNAIASGCPEVSVRE
                     IVMANIFPEYFHCSGFAVYGRATASGTLYHGRVLDYMTEIGLQQAAVTMIVAPENGHR
                     FMNVGYAGFVGSVTGMNDQGLSVGEMGGGGRGRWDGMPMTFMMRKALEDCSTVEQIVQ
                     FWKDTPRTCEYYYVFAQGTPAAAVGVKATSEVVDLIRPGESHPQLGDGIPDTILMSAG
                     SRLKELRKRLETGHGTFTAESALHLMDRPVSMRSNLHNALFAPAELKAWVAHAGARQP
                     AAERPYVSYELGLLFNEAASFSARTP"
BASE COUNT          637 a          909 c          975 g          718 t
ORIGIN      
        1 gaccacgcat acgcaggcac gccatgtctt gccttgggcc atcgccgaga tcaagcgggc
       61 tttcccgcgc gtcagcgtgc atctgcaacc ggtcggcgat ggcgaagtac tggaacgtct
      121 tgcacgcggc gaagcggatt tcgccgtcgt cagcacctcg ggcgaaacgc cggagggtgg
      181 cctcgccgtg cctttgtatc gctggcggcg cctcgtgctg gtgccgcgtt cgcatgcctt
      241 cgcgaacctg cggcgcgcac cgagcctggc cgagctcgcg aacgaacccc tggtgagcta
      301 cgaatcctcg acgcgcgccg attcttcctt ccagcgcagt ttcgccgccg ccggactgcg
      361 cccgaccgtg tcgatgaccg cgcgcgatgc cgacctgatc aagacctatg tgcgcgccgg
      421 gctcggcgtc ggactgctgg cggaaatggc ggtcggtgcc agcgatgacg atctgatcgc
      481 cctgcctgcg ttggacttcg ccgaatgcac gacttgggcg gtgttgccgc gcgaacgcgt
      541 gcgtccggga aaacgctggc ctgatcgaat ctaccgcggc cgcattttga aagagattca
      601 gaaagccggc ggtgaggtgt tgatccgtga tctcggaccg ttgatcgatg cccactatgt
      661 cgaacgagat gacgaagagt gggttgccac catggtcgcc agactcgaac gagatggcct
      721 ggtacgtatc tgcaagcacc gggcgcggct gccgtgatat catcgaatcg tcgaagttct
      781 tgagtcgagc gaattggtgc ttatcgcgcc cataggggag gctggcgaat gacgaacgaa
      841 aaactgtttc gaggcattgc gggccttttt ttcctggtgc tcgtcgttgc cagcagtacc
      901 ccgtttttcg agtttcgggt gctcgacttc tcgcagccgt ttcatgtctg tgcggctcag
      961 agaactcgtc tgctggaagc cattcgcaag tttaatcagg attttccgcc tggtatttcc
     1021 cggcttggcc ccgcagaatt ggctttgttg aagtcgaaag ggtatctcga tgcgtcgttc
     1081 ggttgtccgg gtgacctggt gccgcgtttg ggcagtgggt ttcttcaggc tcgtctcgaa
     1141 gctttggtga tcaatcttgg catcaacatc tcgaccaaag tgccggtgcc aggtggtgag
     1201 tataccggcg tcgatcttgc cacgactgga cgcgtgacgt gttctctgca caaagtgccg
     1261 taggcataga tgaagatgca tcatcaatgc cgtgatgcta tgaagaacag aagagcagag
     1321 ctgtaggaga aagggtatga aactgcagga agtggttctg ggaatcggga tcttcgctgt
     1381 tctcgtcttg atggtgcttt cgtatattcc catcttcggc tggccctacg gcagaatgat
     1441 cgcgcgcgag aaggcctgtt acgcgaacat gcgagtgctg ctcggcgcga ttgaaatgta
     1501 caacatggat cactccgact tcctcgacga gatgaacccg ctggtcatgg caaagttgca
     1561 tacggacggg tacttgaagt cgcccatgac tccgtgcccg ggagatcagg caccgcgcat
     1621 cgggcaccct tttttacagg ctcgtctggc ggtgttggcc gccaatctgg gcgcgtcgca
     1681 ggtcgctttt cggccgattc cgggtggtac gtatgccgga accggacttg cttcggacgg
     1741 ccgcatcgtt tgtaccatgc acggaacggt ggaatgagcc aatcgcagat cagatagcgg
     1801 gagcgaaacg acgatgacag accagacgaa gcagggcaat cagaagacga tgcagggctt
     1861 cggagtgatc ctggtcgcgg tcttcattct gctgtttttc gtattcgggt tcgggattcg
     1921 aaaagcgcgc gtgcatcctc gctcgaaaac ctgctatgcc aatcttcggg tcattatcgg
     1981 ggccatcgaa atgtacaata tggaccacac ccgcatgatg agtacactcg gagcacccga
     2041 actggcacag ttggtctccg ggaaatatct tatcgttccc gtgacctgtc ctggcgattc
     2101 gcggcctcgt ttctcgaaca cgtggataca aggctttttt gaacaactgt cctatcgttt
     2161 cggaagtttc atgatcttgc acacccctgt tccagcgggt tcttaccagg gagcattcct
     2221 cgacacgaca ggaaaaccgt cctgttcgct gcacggaacg gttgattgat cagtcgcctc
     2281 acatgtggac aactgccctg gtatgctcgc gaagcggagt tgtcgggcgc tcacggagtt
     2341 cgagctgaga acgaagctgc ttcattgaac aggagtccga gttcatacga cacgtaaggc
     2401 ctttcggcag ccggttgtcg agcgcctgca tgagcgaccc aggccttgag ttccgcgggg
     2461 gcgaagaggg cgttgtgaag attgctgcgc atcgatacgg gtcgatccat aaggtgcaga
     2521 gctgattcgg ccgtgaaggt tccgtggccg gtttcgagac gtttgcgtag ttccttcagc
     2581 cggctgcctg cggacatcag aatcgtatcc gggattccat cgccaagctg cgggtgagat
     2641 tctccgggcc gaatcagatc gaccacctcg gaagttgcct tgaccccgac cgctgccgcg
     2701 ggtgttccct gggcgaagac atagtaatac tcgcacgtgc gtggggtgtc cttccagaat
     2761 tgtacgatct gctcgactgt agaacagtct tcgagagcct tgcgcatcat gaaggtcatg
     2821 ggcatgccat cccatcgacc acgaccacct ccacccattt ccccgaccga aagcccctga
     2881 tcgttcatgc cggtcaccga cccgacgaat ccggcgtagc cgacgttcat gaatcggtgc
     2941 ccgttttcgg gagcgacgat catggtcacc gccgcctgct gcagaccgat ctcggtcatg
     3001 tagtcgagaa cccgcccgtg atacagggtt ccgcttgccg tggcgcgccc atacaccgca
     3061 aacccggaac aatgaaaata ctcgggaaaa atgttggcca tgacgatttc gcgtactgaa
     3121 acctccggac acccagaggc gatggcattc agttcttcga gataaggtgc cggaatatgc
     3181 ggtctgaggc gggtaaaggc ctcatcgagc acggacggaa accacttgcc gttctgcac
//
LOCUS       DAORHD010000469         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33760_length_3234_cov_4.026423, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000469 DAORHD010000000
VERSION     DAORHD010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33760_length_3234_cov_4.026423"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKO06_10615"
     CDS             complement(<1..621)
                     /locus_tag="PKO06_10615"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNV70140.1"
                     /translation="MRRFSGIRGLILTVISLAFLVATVPADADGFSSQVYGVSAEAFP
                     LIKLGVKVYSPEPIAPTSDSFDLFEKQARITSFTVTMPKQKNYVVLLLDRSSSIEPAM
                     PLVKESAAAFVQAVAGKAELAVHSLGSDFEINQDFTSKTDDLLKSIRGVRPWGGTLLI
                     DSLYKSCEALRNQAGTNDLKTVICLTDGRDETPGGKSPFSSKKPEEL"
     gene            complement(714..2828)
                     /locus_tag="PKO06_10620"
     CDS             complement(714..2828)
                     /locus_tag="PKO06_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV70141.1"
                     /translation="MKISASAQGRTPTRRYWLILFSLFLTAAPLFAESENLKSVHDRY
                     LAAYTAYTQAVSAHAAPETIQRLAGEYETARQAYQQAVGTNTDSPQIATQAASSDISG
                     ESQTESLIETTTQADTTSLSDRSPVSAEEQQRFDQLMKALYAPDRGQSYEKLVGQLQD
                     FLKTARNPKLRLDAIMEIVTLHTEAGHHDRAEKLLQTLAARTSNTEVRRQATTRLKEV
                     QLAREVQRQRAEFTRHNVKCLSLWSQICSTSWFKPVSKVSKLVQFGWNTLGRKWEARK
                     LESLIDEYNEQARKAFPMGSLDQLTNSRLLPGNDITLLCNGRTSFSTRYALARQARET
                     IELQTLLYHNDETGNELADILIERAQAGVEVRVIIDDAFAQGRKDNIIRKMINGGVQV
                     RINNPILAHPLRANFRSHQKVFIIDGTSAVVGGMNIANEYAYGESVEFGWRDTDVLVK
                     GPVVHFIGKLFDENWEELDLERFWPTTPDRTKEVKEKVEKMPILPNRATLIPGPLPVY
                     FAQPPRFQNVRVRFLTTFPRESDDDDILDLFGAYLKACTKEAIFETAYFIPTKPLKEA
                     IIDACRRGVKVKILTNSAESNNHPNAGYAGRSFYRDVIEAGAEVYEWQGAQTLHSKVS
                     LFDDFACTLGAYNVNSRSHSCDSEDVLAIEDRRMAAVFRTMLLKDLARARQVTMSDLD
                     AWKKQIDEALKQNFWRLFTPIF"
BASE COUNT          646 a          877 c          988 g          723 t
ORIGIN      
        1 cagttcttcc ggtttcttcg aggaaaacgg cgatttgccg cccggagtct cgtcgcgacc
       61 gtcggtgaga cagatcacgg ttttcaggtc gttcgtgccg gcctgattgc gcagggcctc
      121 gcatgatttg tacaaagaat cgatgagaag agttccgccc cagggccgaa ctccgcgtat
      181 cgatttcagc agatcgtcgg tcttgctcgt gaagtcctgg ttgatctcga agtcactgcc
      241 cagcgagtgc accgcgagct ccgccttgcc ggcaactgct tgcacgaagg cggcggccga
      301 ttccttgacc agcggcatcg ccggctcgat gctcgacgag cggtcgagca gcagcacgac
      361 atagttcttc tgtttcggca tggtcacggt gaagctggtg atgcgcgcct gtttctcgaa
      421 caggtcgaac gagtcactgg tcggcgcgat cggttcgggc gaatagacct tgacgcccag
      481 cttgatcaac ggaaatgcct cggcgcttac gccatagacc tgcgacgaaa acccgtcagc
      541 gtcggccgga acggttgcga cgaggaacgc cagtgaaatg acggtcagga tgagcccgcg
      601 aataccactg aagcgacgca tgacgaaact ccttaatcgc tgacgatacg acacgagaaa
      661 aaaaacgtgc cagtgacacg atatcaccgg cacgctcgag atgcgaagtc agactagaaa
      721 atgggcgtga acaggcgcca gaagttctgc ttcagggctt cgtcgatctg tttcttccag
      781 gcgtcgagat cagacatcgt gacctgtcgg gcgcgggcga gatctttcag caacatggtg
      841 cgaaacacgg cggccatgcg gcggtcttcg atcgccagca catcttcaga gtcacacgaa
      901 tgactgcgcg agttgacgtt gtatgccccg agcgtgcagg caaagtcatc gaacaaactg
      961 accttggaat gcagggtctg cgctccctgc cactcgtaca cttcggctcc ggcttcgatg
     1021 acgtcgcggt agaacgagcg acccgcatac ccggcgttcg ggtggttgtt gctctcggcc
     1081 gagttggtga gaatcttcac cttgaccccg cgacggcagg cgtcaatgat ggcctctttc
     1141 agcggcttgg tcggaatgaa atacgcggtt tcgaagatcg cttccttcgt gcaggccttg
     1201 agataagcgc cgaagagatc gagaatatcg tcatcatcgg actcgcgcgg aaaggtcgtc
     1261 agaaagcgca cgcgcacatt ctgaaaccgc ggcggctggg cgaagtacac gggtaacggc
     1321 ccgggaatca gtgtggcgcg gttgggcaga atcggcattt tctcgacttt ttccttgacc
     1381 tctttcgtgc gatcgggagt ggtcggccag aatcgctcga gatcgagttc ttcccagttt
     1441 tcgtcgaaga gcttgccgat gaaatgcacg acgggcccct tgaccagcac gtcggtgtcg
     1501 cgccagccga actcgacgct ctcaccgtag gcatactcgt tggcgatgtt catgccaccg
     1561 acgacggctg aggttccgtc aatgatgaac accttctgat gcgagcggaa gttggcgcgc
     1621 agcggatgtg ccagaatcgg gttgttgatg cgcacctgca ctccgccgtt gatcattttg
     1681 cgaatgatgt tgtccttgcg cccctgggca aaggcgtcat cgataatgac ccgcacttcg
     1741 acaccggcct gcgcccgttc gatcagaatg tcggcgagct cgttccccgt ctcatcgtta
     1801 tggtagagca gcgtttgcag ttcgatggtt tcgcgagcct gacgggcaag tgcatagcgg
     1861 gtcgagaaac tggtacgccc gttacagagc agggtaatgt cgttgccggg cagaagacgc
     1921 gaattggtca gctggtcgag cgaacccatc ggaaatgcct tgcgggcctg ctcgttgtat
     1981 tcgtcgatga gtgattcgag cttgcgggct tcccacttgc ggcccagagt gttccagccg
     2041 aattgtacca gtttgctgac tttggaaacc ggtttgaacc acgaggtaga gcagatctga
     2101 ctccacagcg agaggcactt gacgttgtgg cgggtgaact cggcgcgctg acgctgcact
     2161 tcgcgggcga gttgcacttc tttgagacgg gtcgtcgcct gacgacggac ctcggtgttc
     2221 gacgtgcgcg ccgccagcgt ttgcagaagc ttctccgctc ggtcgtgatg tccggcttcc
     2281 gtatgcagcg tcacgatttc catgatggcg tcgagccgaa gtttcgggtt tcgcgcggtt
     2341 ttcaggaaat cctgcagctg acccaccagc ttctcatacg attggccacg atcgggcgca
     2401 tacagggctt tcatcaactg atcgaagcgc tgctgttcct cggcagaaac gggggaacga
     2461 tcggacaggc tcgtggtgtc agcctgagtg gtggtctcga tcagtgattc agtctggctc
     2521 tcaccgctga tgtcgctgct tgcggcctgg gtggcgattt gtggcgaatc ggtgttggtg
     2581 ccaacggctt gctgatacgc ctgtcgcgcc gtttcatact cgcctgccag acgctgaatc
     2641 gtctcgggag cagcatgcgc gctgaccgcc tgagtatagg ccgtgtaggc agccaggtag
     2701 cggtcgtgaa cgctcttgag gttctcgctc tcggcgaaga gtggggcggc cgtgaggaag
     2761 agtgaaaaaa ggatgagcca gtagcgccgg gttggtgttc ttccctgtgc ggatgctgat
     2821 atcttcatga gtttggtttc cctgagaaga gataatcgct gcctgatcag agagggcatt
     2881 cctgtacatt ataaagaaaa atcgacaaac ccgcccgcac tctgttcagc tcagccaaat
     2941 gcacagcttt tcgtgcgtga cgattgtttg ggcactttct gcgcggggct tggttgaaat
     3001 gttcgggaac tcaacagagt gttgagtcgt tgcacagcgt gccgttcact ttttgccagc
     3061 gggtcactga agatcgcgca ttcgagccgt cggcgaatga gcaacaactg catcgggagg
     3121 tgatcttccg ggtgacgcga catgttggca cttgctttgc tttgagccga gaaacatggt
     3181 ttacagtccc ttcaacggag atacgctgac atgcgcaaaa ccaagattct cgtg
//
LOCUS       DAORHD010000470         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33766_length_3234_cov_3.006920, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000470 DAORHD010000000
VERSION     DAORHD010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33766_length_3234_cov_3.006920"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PKO06_10625"
     CDS             complement(<1..683)
                     /locus_tag="PKO06_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HNV70142.1"
                     /translation="MTTISSSRHQQRKAADLPLQVYLIGFPNSGKTALFNALTGSTEE
                     VGNWPGVTQSVVGRRIIHESDTLDVFDLPPCEDLLHAPVHLDQLLSEQPSDNRKVFIQ
                     VIDITRIQEQIAITLDLLTLGFQPLPVLSFCDQVGLEEADKRADEIKRLLGIPVLALS
                     PYRTERFPQFLDVVAHTPYRSLQPQTTAFLQSVQPPQQLAQANWIATKPGDRYKAVRH
                     ELAQADDGRG"
     gene            complement(893..1579)
                     /locus_tag="PKO06_10630"
     CDS             complement(893..1579)
                     /locus_tag="PKO06_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70143.1"
                     /translation="MNDILYNPLLLLFCLLALSFRTTSSSYPGGFLSRDGSLGLAIAL
                     WSLVIAGLVPVFFLDAQIGNRYLLILHSGSALVWFAIFAVFASVLMPPLPASVTGSAS
                     LAALIASPDLLRFDLLFGGLLRFEAPHLSLLLVILPILAILSRLRPVIEPPLPFALWT
                     LFFGLIPASLLLFRNQRSWPSVPAILGGSFGYLLLQQLSNASAVAGSAGALGGLLLVE
                     WVQRQKEQHP"
     gene            complement(1576..2352)
                     /locus_tag="PKO06_10635"
     CDS             complement(1576..2352)
                     /locus_tag="PKO06_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70144.1"
                     /translation="MNHEDPEVASRMATGSPPLPSDQRRLIELSLAIDEAIIQALRLF
                     ALLLLCLLATGELQIQTVRDTTAFGIRFFSPVDTLLMWILGIVGWDLLMFAVTRCTFP
                     TRQVEEHLLSLGLSLTLWRLVFLNLLTLVQPASGTPVAAPASWAAVVLLFVTLATGKE
                     LIARTLLYQDQRLNSPLLAVWGWHHRKDAVFTLIFLVTWSLQMHSSPSSSLVSPAAAA
                     IRFLLLGSATLAVTCRAVTAIHSLNDDHSAMPDQPQGTER"
     gene            2469..>3234
                     /locus_tag="PKO06_10640"
     CDS             2469..>3234
                     /locus_tag="PKO06_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV70145.1"
                     /translation="MTEPATLSWYSRYQWSLIVVGAWIVATLWMSVWQLFSLHWPVSV
                     TMMFGSFVAGATSEGGGAIAFPVFTKLLHIAPATARDFALAIQSIGMTCGSLLIIRSG
                     YSFFPDLVSRVVFGAAVGVCAGLTWLVPFVPPPYPKILFTLFTVCFGVFLAWENFAEC
                     RPRDEEAHVHVELGWGACLVIGLLGGLISSVVGSGADVILFVVLCRRFSCHEKTATRT
                     TIPIMALTSQIGFLFQMTTGKLAPGVLEMWWCAFPIV"
BASE COUNT          723 a          826 c         1019 g          666 t
ORIGIN      
        1 cccctcccgt cgtcagcctg cgccagctcg tggcggacag ccttgtacct gtcgcccggc
       61 ttggtcgcga tccagttcgc ctgcgccagc tgctgcggag gctgcacgga ttgaagaaat
      121 gccgtcgttt gaggttgcag agaacgatag ggggtgtgag cgacgacatc gagaaactgc
      181 ggaaaccgct cggttcgata gggagataac gcgagcacgg gaatgccgag gagtcgtttg
      241 atctcgtctg ctcgcttgtc ggcttcttca agaccgacct gatcacagaa ggagagaacg
      301 gggagtggtt gaaaaccgag cgtgagcaga tcgagtgtga ttgcgatctg ttcctgaatt
      361 cgggtgatgt cgattacctg gatgaagact tttctgttgt ctgaaggctg ttcgctgagg
      421 agctgatcaa ggtggacggg agcgtgcaga agatcctcgc aagggggcag atcgaagaca
      481 tcgagcgtgt ctgactcatg gatgatgcgc cgtccgacga cactctgagt cactccaggc
      541 cagttaccca cttcttcggt tgatccggtc agggcattga acaaggcggt tttaccggaa
      601 ttgggaaaac cgatgaggta gacctgcagc ggaagatcgg cggctttgcg ttgttgatgc
      661 cggctggagc tgatggtagt catggatggt ttgtcaccgc aggggtgact gcgcccacgg
      721 ttggatggga acgagagtga tcagtgaaac ttgactcact acgaaatgcg caactggaaa
      781 cgatgagcga gcaggataca tcggacattt cagggtcgcg gagtgatcat gagccataca
      841 aagggtcagc gcctgtcgat gtctccccgg gatggtccgg taaacggtac gttcatgggt
      901 gttgttcctt ctgtcgctgg acccactcta ccaggaggag accacctagt gcaccggcag
      961 aaccggcaac agctgaagcg ttcgaaagct gctgcagcaa caggtagccg aagcttccac
     1021 ccagaatcgc tggcaccgag ggccaacttc gctgattgcg gaacaacaga aggctggcgg
     1081 gtatcaagcc gaaaaacagg gtccacaggg caaatggcag cggtggttcg atgaccggtc
     1141 gcaatcggct cagaatcgcg agtatcggca gtatcacgag caggagactg agatgtggag
     1201 cttcaaatcg cagcagacca ccgaacagca ggtcgaatcg tagaaggtcc ggtgaagcga
     1261 tcagagctgc aagagaagct gaaccggtga cagaggccgg cagtggcggc atcagcacag
     1321 aggcgaagac agcgaagatg gcgaaccaca caagggcaga gccggaatgc agaatcagca
     1381 gataccgatt gccaatctgt gcgtcgagaa agaaaaccgg aaccagcccg gcgatcacca
     1441 gactccagag agcaatagcc agccccaggc ttccatcgcg actgagaaac ccgccggggt
     1501 aggacgatga agtggttcgg aacgagagtg ccagcaggca gaacagcagc aggagagggt
     1561 tatacaggat gtcattcatc tttctgttcc ctggggttga tctggcatcg cggaatgatc
     1621 gtcattcagc gaatgaatcg ccgtcaccgc ccggcaggtg accgccagag tcgctgaacc
     1681 cagtaacaga aagcgaatag ctgcggcagc gggactaacc agcgatgacg agggcgatga
     1741 gtgcatctgc aacgaccagg taacgagaaa gatcagtgtg aacacggcgt ccttgcggtg
     1801 gtgccagccc cacaccgcga gcaggggtga attcaggcgc tggtcttgat acagcagggt
     1861 gcgagcgatc aattccttgc ccgtggccag ggtgacgaag agaagtacta cggccgccca
     1921 ggatgcgggt gcggcgacag gtgttcccga agccggctgt acgagtgtca ggaggttgag
     1981 gaacaccagt cgccagagag tcagactgag cccgaggctg agcagatgct cttcgacctg
     2041 ccgcgttgga aaagtgcaac gcgtaaccgc gaacatcaga aggtcccagc cgactatacc
     2101 gagtatccac atcagcagcg tatcaaccgg gctgaaaaat cgaatgccga atgctgtcgt
     2161 atcgcgcacc gtctgaatct gcagttcacc agttgccaga agacacagga gcaggagcgc
     2221 aaagagccgc agagcttgta tgatagcctc atcgatcgcc aacgatagtt caatcagtcg
     2281 tctctgatcg ctcggtaatg gcggcgagcc tgttgccatc cgagaagcga cttctgggtc
     2341 ttcgtggttc atcaaccgct cctgagccaa ggcaacaatc cgggtgcacc ggtgacacca
     2401 tggcttgaac aaagaaatct agtctggcga acaagaaagt caaggagatt ttgatacaat
     2461 gggtctctat gactgaacca gcaactcttt cgtggtattc gcgctaccag tggtcgctga
     2521 ttgttgttgg cgcatggatt gtggctacgc tctggatgag cgtctggcaa ttgttctccc
     2581 tgcactggcc ggtgtccgtg acgatgatgt tcggttcgtt tgtcgccgga gcaacgtctg
     2641 aaggcggcgg ggcgattgcg tttccagtct ttacgaagct gttgcatatc gctccggcga
     2701 ctgcgcgtga cttcgccctc gcaattcagt cgatcggcat gacctgcggc agtctgctga
     2761 ttattcgcag cgggtactcc tttttccctg acttggtctc acgggtagtt ttcggtgcgg
     2821 ctgtgggagt gtgcgccggt ctcacctggc ttgttccttt cgtgcctcca ccctacccca
     2881 agattctgtt cacgttgttt accgtttgct ttggtgtctt tctggcctgg gaaaacttcg
     2941 cggaatgtcg accacgcgac gaagaggccc atgtgcatgt tgagttgggc tggggggcct
     3001 gcctggtaat cggcctgctt ggggggctga tttccagcgt tgtcggtagc ggggccgatg
     3061 tgattctgtt cgtggtcctg tgccggcggt tctcgtgcca tgaaaagacc gccaccagaa
     3121 cgacgattcc gatcatggca ctgacgtcgc agattggctt cctgtttcag atgacgacgg
     3181 gaaagctcgc gcccggggtt ctggagatgt ggtggtgcgc gtttccgatc gtgg
//
LOCUS       DAORHD010000471         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33782_length_3233_cov_3.286658, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000471 DAORHD010000000
VERSION     DAORHD010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33782_length_3233_cov_3.286658"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKO06_10645"
     CDS             <1..286
                     /locus_tag="PKO06_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70146.1"
                     /translation="TSDNDFLLALRECSPAEADAFSLFDPSTSPNQRSPYPAQYVIEE
                     LQPGKSHLNMKFAQDSYVIRVKGVVTAHPKAEDVIDEEVIISRFTGVGGP"
     gene            283..1323
                     /locus_tag="PKO06_10650"
     CDS             283..1323
                     /locus_tag="PKO06_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70147.1"
                     /translation="MMNQTRHRIEKRASGFTLTEIIVTIILLAVIFGVAHRVLYQANL
                     ETQKGYWLQQRLTELRNGTRMISQRMKKTSYPSSIVREGGKDVVIAFKEWRRYDDSAR
                     LRDLQINPSTDFDMKSIEGSISPAPEPVSIMTFPVCTPEKDGQAGTITWVELVLHPDP
                     MYNPDRPLGILHLTERETTYTSGPPKYAFGLSSGFSSSLPVRVDRELVHDVSQVSIAS
                     YSVDELRGVMVTEAGSVAKKYRRRYQVSVRIDCMHPRDEKIVLGDQCTVVSNVDLTAM
                     SGGLNVVVLQINGSGPAATADITFNGTPMNVTAGSSFGNGWIVSKIYSNGLSAKHQGS
                     NTSRFFQKKAGS"
     gene            1372..>3233
                     /locus_tag="PKO06_10655"
     CDS             1372..>3233
                     /locus_tag="PKO06_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70148.1"
                     /translation="MNWKNRLIGLLLLLLPVILSLLGRQLLYEDPMQLARERLDERLE
                     TAFAEIEAGRGFAEHFTRTFRQFERQVFSASEPARVLPSLVARIEDTYRVKLQGVLLD
                     GRGVPIREFLDRPVSRQLIQRFFHAYQAFVNTGKPLPSIEQGFVKSFLGRFVPVATQI
                     HGRFLFASADPQRSYLYMSAPRPRGMLFLFFHVETQPTSLAAQELIRTIMSRDPRLRL
                     CFRGVDEVATRTAEALKLPVEQIPEILAALEQSATGSLWLGNTLVKRRILEPSMSVIG
                     AADVLPPLVWWDHWPRVLLGTMLSLGGLLLFVLLLERPFAESVRFKLIGAFLYSSLLP
                     LIIMGLSALSALEEQRRVLERETHQRIENDLFRFDQEFDQFVGTLRGGLMTRYFQDVD
                     GRGASLTTFLEQLERVEKSWRFDTCVVYDRSGTLVFEHQVKGGRPVLGNVVVSFGKLA
                     KTVLSRTIRRAPEATEKSREDSLFESLSASPHLNMIDWGALKFYFGILPFGDLPDSVT
                     HLAALFWHPSRMQHHFLADRLMTFNRYIGDGEIFVWSHTGEGPPLPPDSPRAGILRPL
                     LGRVTSWAVGLRRKVKINDQVYLATGLRFRMLSEFSAIRLTPDLLIQRQVDAVS"
BASE COUNT          620 a          928 c          933 g          752 t
ORIGIN      
        1 cacctcggat aacgacttcc tgctcgcgct gcgcgaatgc tcgcctgccg aagcagatgc
       61 cttctcgctg tttgatccgt cgacgtcgcc aaaccagcgc tcgccctacc cggcgcagta
      121 tgtcatcgaa gagttgcagc ccggcaagtc gcatttgaac atgaagttcg cccaggacag
      181 ctacgtcatt cgggtcaagg gcgtggtgac cgcgcacccc aaggcggaag atgtcattga
      241 tgaagaggtg atcatttctc gctttaccgg cgtgggtggc ccatgatgaa ccagaccaga
      301 caccgcatcg aaaagcgtgc gtccgggttc acgctgaccg aaatcattgt cacgatcatt
      361 cttctggccg tgatctttgg tgtcgcgcat cgcgtgctgt atcaggcgaa cctcgaaacg
      421 cagaagggat actggcttca gcagcgactg accgaactgc gcaacgggac gcgcatgatc
      481 tcacagcgga tgaagaagac ctcctatccg agttcgattg tccgggaagg cggcaaggat
      541 gtggtcatcg cgttcaagga atggcgccgg tatgacgatt cggcccggtt acgtgatctg
      601 cagatcaacc cgtcgaccga cttcgacatg aagtcgatcg aaggaagcat ctctccggct
      661 ccagaacccg tttccattat gacttttccc gtatgcaccc ctgaaaagga tggtcaggct
      721 ggcactatta cctgggttga actggtactc catcctgacc cgatgtacaa ccccgatcga
      781 cctttaggca tcctccatct caccgagcgt gaaaccacct acacctcggg gccgccaaaa
      841 tacgcttttg gtttgtccag tggcttttcc tcatcactcc ccgttcgtgt cgatagagaa
      901 ctcgtacacg atgtcagtca ggtcagtatc gcctcgtact cggtcgatga actgcgtggt
      961 gtgatggtga ccgaagcggg ctcggtcgcg aaaaaatacc gtcgccgcta tcaggtgtcg
     1021 gtgcgtatcg actgcatgca tccgcgtgat gagaaaatcg tcctcggcga tcagtgtact
     1081 gttgtttcaa acgtcgacct gacggcgatg agtggtggct tgaacgtcgt ggtgctgcag
     1141 atcaacggct cggggccggc ggcgaccgcc gacattacct tcaacggcac gccgatgaac
     1201 gtcaccgcgg gttcatcgtt cggcaacggc tggatcgttt ccaagattta cagcaatggc
     1261 ctgtcggcca aacaccaggg cagcaatacc agccgctttt ttcagaaaaa ggccggcagc
     1321 tgatcctcgg ttgccgtggt ccggtgattc atctagaatg agctccggat catgaactgg
     1381 aaaaaccgcc tgatcggtct gctgcttctg ctcttgccgg tgatcctcag cctgctgggc
     1441 cgacagttgc tgtacgagga tccgatgcag ctggctcgag aaagactgga tgaacgcctc
     1501 gagaccgctt ttgcggaaat cgaggctgga cgagggtttg ctgaacactt tacccggaca
     1561 ttccggcagt tcgaacggca ggttttctca gcatctgagc cggcgcgggt tttaccgtcg
     1621 cttgtcgcgc gcatcgaaga cacctatcga gtcaagcttc agggcgtgct tctcgacggt
     1681 cggggggtgc cgatccgtga gtttcttgat cgtccggtgt ctcgccagct gattcagaga
     1741 ttttttcacg cctaccaggc cttcgtcaat acggggaaac ccctgccctc cattgagcag
     1801 ggattcgtga agagctttct tggccgattc gttcccgtcg caactcagat tcatggacgc
     1861 tttctgtttg cctcggctga cccgcagcgc tcctatctct acatgagcgc tccccgtccg
     1921 cgcggcatgc tgtttctctt ctttcacgtc gaaacgcagc ctacgtcatt ggctgcgcag
     1981 gagctcattc gtacaatcat gtcgcgtgac ccccgtctgc gcttgtgctt ccgcggagtc
     2041 gatgaggtcg cgactcgcac ggccgaggcg ttgaagctgc ctgttgagca gattcccgaa
     2101 attctggcag cactggaaca gtcggccacc ggtagcctgt ggctgggaaa cacgctcgtg
     2161 aaacgacgaa tcctggaacc gtcgatgagc gtcatcgggg cagcagatgt cctgcctccc
     2221 ctggtctggt gggatcattg gccgcgggtt ctgctgggaa cgatgctctc gctggggggg
     2281 ctgctgctgt tcgtgttgct gctcgaacgc ccgttcgcgg aatcggtgcg cttcaagctg
     2341 attggggctt ttctctattc atcgctgtta ccgttgatca tcatgggcct ttcagctctg
     2401 tccgcgctcg aggaacagcg tcgggttctc gaacgcgaaa cccaccaacg aatcgagaac
     2461 gatttgtttc gtttcgatca ggagttcgac cagtttgtcg gcactctgcg tggcggtctg
     2521 atgaccagat actttcagga cgttgacggc cgcggcgcga gtctgacgac gtttcttgag
     2581 cagctcgagc gggtagaaaa gtcctggcgg ttcgatacct gcgtggtcta tgaccgaagc
     2641 ggcaccctgg ttttcgaaca tcaggtcaaa gggggccggc ccgtgttggg gaacgtggtc
     2701 gtctcgtttg gcaaactggc gaagacggtg ctttcacgaa cgattcgacg cgcgccggag
     2761 gcgaccgaaa agtcgcgcga agattctctg tttgaaagtc tcagcgcttc tccgcacttg
     2821 aacatgatcg actggggagc cctgaagttt tacttcggca ttctgccgtt cggcgatctg
     2881 cctgattcgg tcacgcacct cgcggcactg ttctggcacc cgtcgcgcat gcaacatcac
     2941 tttcttgccg atcggctcat gacgttcaat cgatacatcg gcgatggcga gatttttgtc
     3001 tggtctcaca ccggtgaagg tccgcccctg ccgcccgatt cgccgagagc cggaatactg
     3061 agaccgctcc tgggacgtgt caccagctgg gcggtcggtc tgcggcggaa agtgaaaatc
     3121 aacgatcagg tgtatctcgc aacggggctg cgtttccgca tgctgtctga gttttcagcg
     3181 attcgcctga cccccgacct gctgattcag cgacaggtcg atgccgtttc gtt
//
LOCUS       DAORHD010000472         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33803_length_3231_cov_4.140113, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000472 DAORHD010000000
VERSION     DAORHD010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33803_length_3231_cov_4.140113"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKO06_10660"
     CDS             <1..318
                     /locus_tag="PKO06_10660"
                     /inference="COORDINATES: protein motif:HMM:NF037773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70149.1"
                     /translation="MAGQEQSFALPPELFRLKLTGLELLSLGETDLEDTLAGKDGYES
                     HLLTCDEQAGQYHKIVTRSGKVFGAILLGPCPFSRLVEKAVQERRDWSALQNEIKATI
                     ERK"
     gene            323..850
                     /locus_tag="PKO06_10665"
     CDS             323..850
                     /locus_tag="PKO06_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV70150.1"
                     /translation="MHCPKCAIDLNDDVPKCPSCGFCIDDLNADLGTPKEKTGLIMDW
                     AGVISDDAKQKLNGRLQHFATRTGSEFLIATIQTTTPRLPSEYVFWLLNRWQVGGPTH
                     SGLLLLLALQERRIEVEVGYSLERFVTDDAATLILQNHAVPFLKAGDVDGGLFHASDV
                     LARIVEEGLDEEKKS"
     gene            847..2004
                     /locus_tag="PKO06_10670"
     CDS             847..2004
                     /locus_tag="PKO06_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV70151.1"
                     /translation="MRRISGYRRLYLTVFLISLSAILSAGVHPPGDWPVRDEIHQLDT
                     NSLFSLRRICTDLKRDTGAELVVLITRTTSGEDPQRYGVRLFNNWGIGRAGIDDGVLL
                     MFAMSDRRGEIIPGNRYKSLLNSSVSADLIRQHAIPSIKAGRPNDGVINTANEIARQV
                     RDYERTRKGYATPAPQPAGKRSSSVWPTTSTTSRSTQRPSVTRRPSSSPTPSAPPISG
                     WFGPTMLPILRVVFLAGAVVWAAAVIFYFFTAFSSGVLLFPKTPALLGFGLSGAALTF
                     LGFRLLSVHAGVLDQFAAGSGGVGLLTLLFCGSHICPRCNKWMSITRVTLVSATYSSS
                     GRGECTEHCEHCRYHNVYTYTIPRRTRSRSSSGSRRSGGGRSSGGGGGASW"
     gene            complement(2112..3230)
                     /locus_tag="PKO06_10675"
     CDS             complement(2112..3230)
                     /locus_tag="PKO06_10675"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNV70152.1"
                     /translation="MSTLDVVRQQPDHLQVTALAAGRNWKRLAEQVRQFKPRLAVISE
                     PAFLDKLREAVRDLDVEVACGMEGLRKAATCEAADTLVSALVGSIGLEPVLAGIHAKK
                     TICLANKETLVVAGRLVMQEARRAGVPIIPIDSEHSAIFQCLADRDKKHVQRLILTAS
                     GGPFRQWSADQMSRATPQDALKHPNWDMGGKITIDSATLMNKGLEVIEARWLFDIEFD
                     RIEVLVHPQSVIHSMVEFVDGSVLGQMGAPDMRGPIQYALSFPNRWQAACHKLDLLKV
                     GALTFESPRRGDFPCLDFAFEAGRRGGTLPCVMNAANEVAVDAFLKGRLTFSGIAKLI
                     RRIMDIMPFQSEPNLDDLLAADREARIKAQELLPSVNA"
BASE COUNT          643 a          996 c          926 g          666 t
ORIGIN      
        1 atggccgggc aggagcagtc gttcgccctg ccccccgagt tgtttcggtt gaagctgacc
       61 gggctcgaac tgctgagtct cggtgaaacc gatctggaag atacactcgc cggcaaagac
      121 ggctatgaga gtcatctgct gacctgcgac gagcaggccg gtcagtatca taagatcgtg
      181 acccgttccg gcaaagtgtt cggcgccatt ctcctgggcc cctgcccctt cagtcggctg
      241 gtcgagaaag cggtgcagga gcgccgcgac tggtccgcgt tacagaacga aatcaaagcg
      301 actatcgaga ggaagtagcc gcatgcactg ccccaagtgt gcgatcgacc tcaacgacga
      361 tgttccgaaa tgtccgagtt gtggtttctg catcgacgat ctgaatgctg atttgggcac
      421 acccaaagag aaaaccggcc tgatcatgga ctgggcgggt gtcatttctg acgacgcgaa
      481 acaaaaactg aacggccgcc tgcagcattt cgccacccgc acgggatcgg agtttctgat
      541 cgcaaccatt cagaccacca ctccgcgact gccgagcgag tacgtcttct ggttgctcaa
      601 tcgctggcag gtcggggggc caacgcactc gggcctgctg ctactgctcg cccttcagga
      661 acgccgcatc gaggttgaag tcgggtattc tctggaacga ttcgtcaccg acgacgctgc
      721 caccctgatc ctgcagaacc atgccgtccc ttttctgaaa gccggtgatg tcgacggcgg
      781 cctgtttcac gcttctgacg ttctggcccg tatcgtggaa gaaggactcg acgaggaaaa
      841 gaagtcatga gaaggatttc cggttaccgc cgactgtatc tcaccgtctt tctgatttcg
      901 ctgtcggcaa tactctctgc tggagtgcat ccgccaggtg attggccggt acgcgacgaa
      961 attcaccagc tcgacaccaa ttctctgttt tcgctgcggc gcatctgcac tgatctcaaa
     1021 cgtgacacgg gcgccgaact cgtggttctg atcacgcgca ccacgtcggg cgaagacccg
     1081 caacgctacg gcgtgcgtct gttcaacaac tggggcatcg gtcgggccgg gatcgatgac
     1141 ggagtattgc tgatgttcgc catgagcgat cggcgcggcg aaatcattcc gggcaaccgg
     1201 tacaaaagtc tgctgaactc atccgtctct gccgatttga tcagacagca cgcgattccg
     1261 agcatcaagg ctggccgccc caacgatggc gtgatcaata ccgcgaacga gatcgctcgt
     1321 caggttcgcg actacgaacg cactcgtaaa ggctatgcga ctcccgcgcc tcaacccgca
     1381 ggaaaaagaa gctcttccgt ctggccgacc acctcgacca cgtcacgttc cacgcagcgc
     1441 ccttcggtca cccgacgccc gtcctcgtca ccaacaccct ctgctccccc gatctcaggg
     1501 tggttcgggc cgaccatgct cccgattctg cgcgttgtct ttcttgccgg ggcagtcgtc
     1561 tgggctgctg ccgtcatctt ctatttcttt acggcattca gcagcggcgt gctgctcttt
     1621 cccaagacac cggctctgtt ggggttcggc ttgtctggag ccgctctcac gttcctgggg
     1681 ttccgtttgc tgagcgttca tgccggagtt ctcgatcagt tcgcggcggg atcaggcggc
     1741 gtcggcctgc tcaccctgtt gttctgcggc agtcatatct gcccgcgctg caacaagtgg
     1801 atgtcgatca cacgcgtcac gctcgtttcg gccacgtatt cttcatccgg ccgcggtgaa
     1861 tgcaccgaac attgcgaaca ctgtcgctat cacaacgtgt atacgtatac cattcctcgc
     1921 cgtacgcgca gtcgctcgtc atcgggatcg cggcgttccg gcggtggacg ttcatcgggg
     1981 ggtggcggcg gcgctagttg gtagacatcg cgacccgacg cgtcgtcgaa gacagccgag
     2041 cgctctcgcc tggcacgagg gctacgaaga gagacctgag ccagaggacg attacctggc
     2101 acagatcggt ttcaagcgtt gaccgacggg agaagttcct gggctttgat gcgcgcttcg
     2161 cgatcagcgg cgagcagatc gtccaggttg ggttctgact gaaacggcat aatatccatg
     2221 attcgacgaa tcagctttgc gataccggaa aacgtcagtc gacccttgag aaaagcatcg
     2281 accgccacct cgttcgccgc gttcatgaca cagggcagcg tgccaccccg tctgccggcc
     2341 tcgaaggcaa agtcgaggca cgggaaatcc ccgcggcgag gactctcgaa cgtcaacgct
     2401 ccgaccttga gcagatcaag cttgtgacag gcggcctgcc agcgattggg aaaactcaac
     2461 gcatactgaa tcgggccgcg catgtcgggt gcgcccattt gtcccaggac cgatccatcg
     2521 acgaattcga ccatcgagtg aatgaccgat tgcggatgca caagcacctc gatacgatcg
     2581 aactcgatgt cgaacagcca gcgagcctcg atgacttcga gccctttgtt catcagcgtt
     2641 gccgagtcaa tcgtgatctt tccccccatg tcccagttcg ggtgtttcag cgcatcctgc
     2701 ggggtcgctc gactcatctg atcggctgac cattgacgaa acggaccgcc agatgccgtc
     2761 agaatgagac gttgcacatg tttcttgtcg cgatctgcca ggcactgaaa gatcgccgaa
     2821 tgctcagaat cgatcggaat aatcggaacg cccgcacggc gcgcttcctg catgaccaga
     2881 cggccggcaa cgaccagggt ttccttgttg gcgaggcaga tggtcttctt cgcgtgtatc
     2941 ccggccaaca cgggttcgag cccgatcgag ccgacgagcg cggaaaccag ggtgtcagcc
     3001 gcttcgcagg tggccgcttt ccgcaagccc tccatcccgc aggcaacctc gacgtcgaga
     3061 tcacgcacgg cttcacgcag tttgtccaga aacgcgggtt ccgaaatcac ggccagacgc
     3121 ggcttgaact ggcgcacttg ttccgccaga cgtttccagt tgcggccggc ggccagagcg
     3181 gtcacctgca gatgatcagg ctgctgacgc acgacatcca gcgtgctcat g
//
LOCUS       DAORHD010000473         3229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33846_length_3229_cov_3.983302, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000473 DAORHD010000000
VERSION     DAORHD010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33846_length_3229_cov_3.983302"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PKO06_10680"
     CDS             complement(<1..627)
                     /locus_tag="PKO06_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNV70153.1"
                     /translation="MTLTIAGWIGAILGFGFLVLIHEIGHFLALRWFGLPVYAFSVGF
                     GSPLWKKQLGQTEYRLGWLPLGGYVMPEDPDVAEKREQEGGSLLPDHPPAAQFWVALA
                     GPVANIVLGFVLFMTLMVGWGEPTPGLTVDSIVKGSPAAVAGLQPDDRLVSINGVTLK
                     NWPDFIAQIQLNRQRSSLIGIERLGREIAIPITAVQEGERFVLGVRPRF"
     gene            complement(643..>3229)
                     /locus_tag="PKO06_10685"
     CDS             complement(643..>3229)
                     /locus_tag="PKO06_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70154.1"
                     /translation="PPEVVTPPDPEPDPLVTAQPPTNPDKPPTDPKTPDNQPDSPIDK
                     PPPDEPGPGDDSDNPPDKPIPPMTGVKPPPEPEPISTEDPEQKKIKEAETLLGHARRK
                     YQDQLYDDAEGILKKAVELCPGVIGADDLQKQIDEKRKMRKDFKLAISLYENKKYDLA
                     RDPILKAYTDEPDKYPEAPFYLGKIFLLGGQKDLKKAQEYFDIYLKSEKADPDLKRDV
                     EWVVIGILADDEQYEKAYERFNDFAEREPEYAKNRETFWKLKYWLWYKNNPTAIHIGL
                     ACFLGAFVLVFLLMILPSFNILVRDPVAKAQKAFAAKNYQKAVQIAEDFLKRGKQPVQ
                     IQRQVLEVLIESHYHLKNFFKCQGYGKELLQLFPDNIVAWTFLAKAYAETNDSSDEAI
                     TMYEALYKKNPENKQYLPTLCKYYATHKQYTLESLEIMYAFFQKEPGNKENTLALAEG
                     MVQTKRLSDEVIPVLLEALKAQPKNLDFRELLARNFSKKNQYAEAVKECLQVLDGNIN
                     NMGIHVVYTTCMKKLNMLEEAVLQYEEFIRKYPKNVQIVEIVTGLRKELEQANRATLA
                     PSAGLSPFSDDLIVEGIPTPGGSKDLGLEGFVEPPPEGFDPNAGEVPLPDFLKAERQG
                     QSGQPSAAPAPEMSETTVPAKTPTTNALEDIPTLDPFATETAGMGTPFADLDLPPEKP
                     QGVKTPAPAASPAPPEPEVPTEALEHLSGDADLSSEVPGSEPPEPVPERSPSASPQTV
                     DLDECQRKLQQKKGKEVIENLAPVFAGQRSKPVGVLLADAYLQTKQADLAKEILETID
                     CDWEMMSEDLKDICYRVGVALEEQKKPDEALILYDKICNVDIDFRDTFDRSDRLYSER
                     KKKKH"
BASE COUNT          633 a          910 c          884 g          802 t
ORIGIN      
        1 gaaccggggg cgcaccccga gaacaaatcg ctcgccttcc tgcactgcag taatcggaat
       61 cgcgatctcc cgtcccagac gttcgatgcc gatcaggctc gaccgctgac gattcagctg
      121 aatctgcgcg ataaaatccg gccagttttt cagggtcaca ccattgatgc tcacgagccg
      181 atcatcgggc tgcagacccg cgaccgctgc cgggctgcct ttcacgatcg agtcgacggt
      241 cagtccgggg gtcggttcac cccagccgac catcagggtc atgaagagca caaaacccag
      301 aacaatgttg gcgaccggcc cggcaagagc gacccagaac tgcgctgcgg gcggatgatc
      361 cggcagcagc gagccgcctt cctgctcccg cttttccgcc acatcagggt cttcgggcat
      421 gacgtagccg cccagcggca accagccaag acgatactcc gtttgtccca gctgcttttt
      481 ccacaatggc gacccaaagc cgacagagaa cgcatacacg ggcaacccaa accagcgcag
      541 agcaagaaag tgtccgatct cgtgaatgag tactagaaag ccaaagccga gaatcgctcc
      601 tatccagccc gctattgtga gtgtcatgaa atgtgttcct tgtcagtgtt tctttttctt
      661 gcgttcggaa taaagtcgat cagagcgatc gaaggtatcg cgaaaatcga tatcgacgtt
      721 acagatcttg tcatacagaa tcaaggcctc atccggcttt ttctgttcct caagcgccac
      781 gcccacacga tagcagatgt ctttcagatc ttccgacatc atctcccaat cgcagtcgat
      841 ggtctcgaga atctccttcg ccaggtccgc ctgcttggtc tgcagatagg catccgccag
      901 cagcactccc accggcttgc tgcgctggcc ggcgaagacg ggcgcgaggt tttcaatcac
      961 ttccttgccc ttcttctgct gcagtttacg ctggcattca tcgagatcga cggtttgcgg
     1021 agaagcgctc ggcgagcgtt ccggcacagg ctccggcggc tctgacccag gcacctcgct
     1081 cgacagatct gcatcgcctg agagatgctc gagcgcctct gtcggcacct cgggttcggg
     1141 aggcgcgggc gaagccgcag gggccggcgt cttcacgccc tgtggtttct ccggtgggag
     1201 atcgaggtcg gcaaacggag ttcccatccc agcggtctca gtggcaaagg ggtcgagcgt
     1261 cggtatgtct tcgagagcgt tcgtcgtggg cgttttcgcc ggaactgtcg tctcagacat
     1321 ttctggtgcc ggcgcggcag agggttgtcc gctctgaccc tgacgttccg ctttgagaaa
     1381 gtctggcaga ggcacttcac cggcattcgg atcaaagcct tcgggcggtg gttcgacgaa
     1441 gccctcgaga ccgaggtctt tgcttccccc cggagtcgga ataccttcca cgataaggtc
     1501 gtcagagaac ggcgagagcc cagcactggg agccaacgtg gcccgattcg cctgttcgag
     1561 ttctttgcgc aatccggtca cgatctcaac aatctgcacg ttcttgggat acttgcgaat
     1621 gaactcctca tactgcagca ctgcttcttc cagcatgttg agtttcttca tgcaggtcgt
     1681 atacacgacg tgaatgccca tgttgttgat attgccgtcc agaacctgca gacattcttt
     1741 cacggcctcg gcgtattgat ttttcttcga aaagttgcga gccagcagtt cacgaaagtc
     1801 caggttcttc ggctgcgctt tcagcgcctc gagcaagact ggaattactt catccgagag
     1861 tctcttggtt tgcaccatgc cttcggcgag ggccagcgtg ttttccttgt ttcccggctc
     1921 tttctggaaa aacgcataca tgatttcgag cgactcgagc gtgtattgtt tgtgagtcgc
     1981 atagtatttg cagagcgtcg gcaggtactg cttgttctcc ggattcttct tgtagagtgc
     2041 ctcgtacatg gtgatggctt cgtctgaaga atcgttggtc tcggcgtatg ccttggccag
     2101 aaaagtccag gcgacgatgt tgtcagggaa gagctgcagc agctctttgc cgtaaccctg
     2161 acatttgaag aagttcttga gatggtagtg cgattcgatg aggacttcga gcacctgtcg
     2221 ctgaatttgt accggctgct tgcctcgctt gagaaagtcc tcggctatct gcaccgcttt
     2281 ctgataattc ttggcggcaa aggctttctg ggccttggcc acgggatctc tgaccagaat
     2341 gttgaatgaa ggcagaatca tcagcagaaa gaccaggaca aaggccccga ggaaacacgc
     2401 aagaccgatg tgaatggccg tcggattatt cttgtaccag agccagtatt tcagtttcca
     2461 gaacgtttca cggtttttcg catattcagg ttcacgttcc gcaaagtcgt tgaaacgttc
     2521 ataggctttt tcatactgct catcatccgc cagaatacca atgacgaccc actcgacatc
     2581 acgtttcagg tcggggtcgg ctttttcact tttcagatag atgtcaaagt attcctgagc
     2641 ctttttcagg tctttctgcc ccccgagcag aaaaatcttt cccaggtaga acggggcttc
     2701 cgggtattta tccggttcat cggtataggc tttcagaatc ggatcacgag ccagatcgta
     2761 cttcttgttc tcatacaggc tgatcgccag cttgaaatcc ttgcgcatct tgcgcttctc
     2821 atcgatctgt ttctgcaagt cgtcggcccc aatcacccct gggcacagtt cgactgcctt
     2881 tttcagaata ccctcggcat cgtcatagag ttgatcctgg tatttgcgcc gcgcgtgacc
     2941 aagcagggtt tccgcctctt tgatcttttt ctgttcagga tcttccgtcg atatcggttc
     3001 tggttccggt ggtggcttca ccccggtcat cggtgggatc ggcttgtccg gtggattgtc
     3061 cgagtcatca ccaggccctg gctcgtccgg cggcggtttg tcaatgggtg agtctggttg
     3121 attgtccggt gtcttgggat ccgtgggtgg cttgtccggg tttgtcggcg gctgcgccgt
     3181 gaccagagga tcaggctcgg ggtccggagg ggtcaccact tccggtgga
//
LOCUS       DAORHD010000474         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33902_length_3226_cov_3.677073, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000474 DAORHD010000000
VERSION     DAORHD010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33902_length_3226_cov_3.677073"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1751)
                     /locus_tag="PKO06_10690"
     CDS             complement(<1..1751)
                     /locus_tag="PKO06_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70155.1"
                     /translation="MINRLRSISWTGMMPLLLALFPLFIFWRGAAWETQQLTREREDQ
                     WLRTADRGARWLKKAGEASYWAELTARRFGTHSRRLLEERAPRSQPPSRALLQQVARE
                     AAGRMHPAGMPQCRLWVLPLDPEIPGGRPALLSGPALADRPRHLMTSILDAVGEEWRG
                     RASPLAQREWQNRMEHTFGFGVSGELFVPGYRGTSIPVVFNRAYGNLVWNVITWREKP
                     VAIYILISPLLDVHQRRIARLLLQHYPRLVPKSPVYPVLLPLAPWNNERPPQLYAPKS
                     RRVASSLVLARKLQHEIRIQNLPASPPDPMIDGLKVKTAQVPFYLYGEQIKGRVHLPV
                     RRMNQVFRDDRDLWWSMLTPLPPRLGAVSLLIGDAPPRRLGVLEVTGFSWSFLCLVVL
                     LVVAGEALWFRRSLTLGARASLLAWFFGLALIPLSLSIENSLQFVSDLRESLYDDLRY
                     KLEQTVQSIEAGNNQIAEKTIAACDTFFKSHRTWNPDQFKTTLSGNPDALRRYVETHS
                     QIPGFQLDGLFVYGGNDFEHSYIDPAVPAASKRLFSTALKNLLKTKLSLERSTGREPT
                     TAEIMAANNMSWHIGDVS"
     gene            complement(1748..2614)
                     /gene="mutM"
                     /locus_tag="PKO06_10695"
     CDS             complement(1748..2614)
                     /gene="mutM"
                     /locus_tag="PKO06_10695"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HNV70156.1"
                     /translation="MPELPEVETLAALLRPLIVGKRVLQCQASVPRLRLPLPTRLPFE
                     WHNWEILDIRRRGKYLLVPGPADQVLVIHLGMTGWLSVSDSFFPADRHERWRLLFGDG
                     SSLRFADPRKFGQIFFSKFSTPDTIAGLPELGVEPLSSRFTGSLLYALSRRVRRPLKP
                     WLLDQSVIVGIGNIYASESLFQARLSPTRHVASLTREECLRLCRAIRHVLRDSIAAGS
                     TCAQAIPQPTENTTHYTLSLHVYEREGCNCSRCGAGIIHRLVQSGRSTYWCPECQSAA
                     GRSARQRSRNES"
     gene            complement(2620..>3226)
                     /locus_tag="PKO06_10700"
     CDS             complement(2620..>3226)
                     /locus_tag="PKO06_10700"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70157.1"
                     /translation="YPMPQPDHALRAVKCAIAMQKRLEELRPIWKQRGLPDVYMRAGV
                     NTADCMVGFFGSDVQMNFTCLGDGVNLASRLEGANKAYHTLMMMAEATARHLENSGIR
                     YRFLDFLAVKGKLKPMRVFEIIGEHGDRDELWQKVIPVYDEGITAYLNRDWDKAQAAF
                     ESILLLIPEDGPSLTYIERCQAFREAPPPPDWDGRFILKTK"
BASE COUNT          706 a          883 c         1025 g          612 t
ORIGIN      
        1 gagacatcgc cgatgtgcca ggacatatta ttggcggcca tgatctcggc cgtggtcggt
       61 tcgcgtcccg tcgaacgttc aagagacagc ttggtcttga ggaggttttt cagggccgtc
      121 gaaaacaatc gcttcgaagc cgccggaacg gctggatcga tgtaggaatg ctcgaaatcg
      181 ttgccgccat agacgaacaa accgtcaagt tgaaagccgg gaatctggct gtgcgtctca
      241 acataccggc gcagagcgtc cgggttacca gacaacgtcg ttttaaactg gtctggattc
      301 cacgtccggt gtgacttgaa aaacgtgtca caggcggcga tagtcttctc ggcgatctga
      361 ttgttcccgg cctcgatgga ttgtaccgtc tgctccagtt tgtaccgcag atcatcatac
      421 agcgactcgc gcagatcgct gacaaactgc agactgtttt cgatactcag cgacaggggt
      481 atcaaggcaa ggccgaaaaa ccaggctaat agagaagctc tggcccccag ggtcagagaa
      541 cgtcgaaacc agagcgcttc accggcgacg acgagcagca cgacgagaca caggaaactc
      601 cacgagaaac cggtgacctc aagaactccg agacgacgcg gcggggcgtc accgatgagc
      661 agactgacgg cacccaatcg aggggggaga ggagtgagca tgctccacca gaggtctcga
      721 tcatctcgaa agacctggtt catgcgtcgc acggggaggt gtacgcgacc cttgatctgt
      781 tcgccgtaga gataaaacgg cacctgagca gttttgactt tcagaccgtc gatcatggga
      841 tcaggaggtg aggccggtag attctgaatg cgaatctcgt gctgcagctt tcgggccagc
      901 actaaggacg atgccacacg acgacttttc ggagcgtaca gctgaggggg gcgttcgttg
      961 ttccagggcg cgagcggcag aagaacggga tacacggggc ttttcggcac gaggcggggg
     1021 tagtgttgca gcaggaggcg agcgatacgt cgctggtgaa cgtccaggag tggagaaatg
     1081 agaatataga tggcgacggg tttctcacgc caggtaatca cgttccagac caggttgcca
     1141 tacgcgcgat tgaacacgac ggggatgctg gtgccccggt agcccggtac gaacagctct
     1201 ccggaaacgc cgaatccgaa tgtgtgctcc atgcggttct gccactctcg ctgagccagt
     1261 ggtgaggcgc gcccgcgcca ttcctcgccc acggcatcga gaatcgaggt catcaggtgc
     1321 cggggccgat cggcgagagc cggtccggac aggagtgccg gtcgaccgcc gggaatctca
     1381 ggatcgagag gcagaaccca cagacggcac tggggcatac cggcggggtg catgcgcccc
     1441 gctgcttccc tggccacctg ctgcaaaagc gctctcgaag gcggttgtga gcgaggcgca
     1501 cgttcctcga gaagacgacg ggaatgagtt ccgaaccggc gggcggtgag ttcggcccag
     1561 tacgaggctt cgccggcttt cttgagccag cgggccccgc ggtccgccgt acgcaaccac
     1621 tggtcttcgc gttcgcgggt cagctgctgg gtctcccagg ctgcgccgcg ccagaagatg
     1681 aacaagggaa acagggccag cagcaggggc atcatgcctg tccaggagat ggaacgaagc
     1741 cggttgatca tgattcgttc cgtgatcgtt gccgcgcgga tctgccggca gcggattggc
     1801 actctgggca ccagtaagtt gaacggcctg attgtacaag tcgatggatg atgcctgcgc
     1861 cgcagcgaga gcagttgcat ccttcgcgtt catacacgtg cagagacaac gtatagtgag
     1921 tcgtattctc ggtcggctgg ggaattgcct gcgcgcaggt gctgccggcg gcgatcgaat
     1981 cgcgtagaac gtgtcgaatg gcacgacaga gcctgaggca ctcttcccgt gtcagactcg
     2041 cgacgtgccg cgtcggagac agacgtgcct ggaacaggga ctccgaggca tagatgttgc
     2101 cgatgccgac gatgactgac tgatcgagca gccagggttt caggggacgc cgaacgcgac
     2161 gcgacagggc atagagcaga cttccggtga agcgggaact caagggttcg accccgagtt
     2221 cgggtagccc tgcaatggtg tccggtgtgg aaaacttcga gaagaagatc tgaccaaact
     2281 ttcgcggatc agcgaagcgc agagaagaac cgtcgccaaa gagcagacgc catcgttcgt
     2341 gacgatcggc aggaaagaag gagtcgctca cggacaacca gcccgtcatc ccgagatgaa
     2401 tgaccaggac ttggtccgcc ggtccgggaa ccaacagata ctttcctctt cgcctgatat
     2461 ccaggatctc ccagttgtgc cactcgaacg gcaaacgggt gggtaacgga agtcgcagtc
     2521 ggggcacgct ggcctggcat tgaagaaccc gcttgccgac gatcagtggg cgcaggagag
     2581 cggccagggt ttcgacttcg ggaagctctg gcatgatgat tacttggtct tgaggatgaa
     2641 gcgaccgtcc cagtccggcg gcggcggagc ttctctgaag gcctgacagc gctcgatgta
     2701 agtcaggctt ggaccgtctt cgggaatcag cagcagaatg gattcaaagg cagcctgggc
     2761 tttgtcccag tcgcgattga gataggcggt gatgccttca tcatagacgg ggatgacttt
     2821 ctgccagagt tcatcacgat ctccgtgttc gccgatgatt tcgaaaacgc gcatgggttt
     2881 gagcttgccc ttgaccgcga gaaaatcgag aaaccgatat ctgatgccgc tgttttcgag
     2941 gtgtcgcgcc gttgcctcgg ccatcatcat cagagtgtga taggctttgt tcgcgccttc
     3001 cagacgcgaa gccaggttga cgccgtcacc gagacacgtg aagttcatct gcacatccga
     3061 accgaagaac ccgaccatgc agtcagccgt gttcactccg gctcgcatat agacatcggg
     3121 caagccacgc tgtttccaga tcgggcgcag ttcttccaga cgtttctgca tggcgatcgc
     3181 acactttacc gctctcagcg cgtgatccgg ttgcggcatg gggtag
//
LOCUS       DAORHD010000475         3225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33918_length_3225_cov_3.157413, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000475 DAORHD010000000
VERSION     DAORHD010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33918_length_3225_cov_3.157413"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKO06_10705"
     CDS             complement(<1..339)
                     /locus_tag="PKO06_10705"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV70158.1"
                     /translation="MNEPRPTTLVDHLEELRSRLLWSLAAYVMAFFVCYSQTDFLLNL
                     VMKPLRVTYPDIRLTALTITEGFFTTMKLAAWAALILVIPVFSWHLWRFVSPGLLPRE
                     RRFAAWSLLPM"
     gene            516..755
                     /locus_tag="PKO06_10710"
     CDS             516..755
                     /locus_tag="PKO06_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 family protein"
                     /protein_id="HNV70159.1"
                     /translation="MWLIRKLFWLCTLPFIALFGVLMFAFPEEMKEFKKDLVDMVENS
                     WLRWIGFVAFVVAMVHLVKFFDGKHTLVRLTCPED"
     gene            complement(817..1473)
                     /locus_tag="PKO06_10715"
     CDS             complement(817..1473)
                     /locus_tag="PKO06_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70160.1"
                     /translation="MKHTRALLIAALMMGSLTFSVVGQNAQRPGQPPPPPALPSAIRS
                     GPVAGTPQSPQADQAARTFITQGQGYLAEGRLPEAKEALRTAIKLEPMNLEAWSWYDY
                     VVETSYVKRARDEKQNPVIERDLKPTFSIDNVDSYREYNTMYIVGEVKNTSDSLKSRI
                     ELTGTLLDDQNEILRKSTVGLTLKSRGLFPSEVSLFEIPFPDPPPGVKTMRVRVTHFE
                     "
     gene            complement(1536..2234)
                     /locus_tag="PKO06_10720"
     CDS             complement(1536..2234)
                     /locus_tag="PKO06_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70161.1"
                     /translation="MQLYIQIVETFLDQPAGVLSIHQIAQRLELPYGTAYNRTHQLGG
                     MGVLRIQPQGKAKLCSLNAANPMTGNLLALGAAQRAHQFMMSGSPLADVISKVRSLIE
                     ASSGDLLQSALILNLDHLKEIEIPVINWENSSIDGQASTEPEVTLPLDLFLITDEKQE
                     LETLQQRIESLAPPGLSIKPTRMQVCPTNLIEMLRAEENDAGIAAYQILRRALPILGF
                     ERFFSIILRAFPAH"
     gene            2677..>3225
                     /locus_tag="PKO06_10725"
     CDS             2677..>3225
                     /locus_tag="PKO06_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70162.1"
                     /translation="MADKRVFRDIPNLIEIQTQSFEWFLQPGKPLSERKKQGLQEVFH
                     EIFPIQDYVGNLCLEFIDYSLGLGMCKNCPQIKLNKKCLYDDCSFPEKECEIAFVGDN
                     PFRVKHSPDQCRKRDLTYSIPLNIKVQLIMQQTGEVKEQEIFLVNLPCMTERGTFIIN
                     GAERVVVSQLHRSPGVYFSYNRP"
BASE COUNT          721 a          808 c          951 g          745 t
ORIGIN      
        1 catcggcaga agggaccagg cggcgaatcg tcgctcacga ggcagcagac cggggctgac
       61 aaaccgccac aggtgccagg aaaataccgg gatgaccagg atcagagcag cccaggccgc
      121 gagcttcatg gtcgtaaaaa aaccttcggt aatggtcagc gctgtcaggc gaatgtcggg
      181 ataggtgacc cggagtggct tcatgacgag gttcaagagg aagtccgtct gcgagtaaca
      241 cacgaaaaac gccatcacat acgccgctaa actccacaag agacggctgc gcagctcttc
      301 gaggtggtcg accagagttg tggggcgtgg ttcgttcatg tcaagatgcg ggcattatag
      361 gggggccggt ttcccgtggt caatgatcag gttcgtcgct gctgagatgg aacattgtcg
      421 ttgacaagga tctctgtttc aggtaaaaag atcacaaatg aggtaaaggg cagacctttg
      481 ccgacaacca tttctgacca tcagggagat acaccgtgtg gcttattaga aagctgttct
      541 ggctgtgcac gcttccgttc atcgcgttgt tcggggttct tatgtttgcg tttcccgaag
      601 agatgaagga attcaaaaag gatctggtcg acatggtcga gaattcctgg ttgcgctgga
      661 tcggttttgt cgcctttgtt gtcgccatgg tccatctggt gaagttcttc gatggcaagc
      721 acacgctggt tcgtctgacc tgccccgaag actgatcaca tcgatccgaa agaaaatcac
      781 ggaggccatg gggcttccgt gatttttttg tcggtgttac tcgaagtgag tcacccgtac
      841 tcgcatggtt ttaacccctg gaggtggatc cgggaagggg atctcgaaga gcgagacctc
      901 ggacggaaac agtccgcgac ttttcaaggt caaaccgacc gtcgacttgc gtagaatttc
      961 attctggtcg tcgagaagag tcccagtgag ctcgatgcgg ctcttgagag agtctgaggt
     1021 gtttttcacc tctcccacga tgtacatggt gttgtattct cgataagagt ccacgttgtc
     1081 aattgagaag gttggcttca gatcgcgctc aatgacgggg ttctgtttct cgtcgcgggc
     1141 ccgcttcacg tagctcgttt cgaccacgta atcgtaccag gaccaagcct cgagattcat
     1201 gggctcgagc ttgattgcag ttcgcagagc ctctttggct tcaggtaatc gcccttcggc
     1261 gagatatccc tgcccctggg taatgaacgt gcgtgcagcc tgatctgcct gaggtgactg
     1321 cggcgtgccg gcgaccggcc ccgagcggat cgcggaagga agcgcgggag gcgggggcgg
     1381 ctgcccaggc cgctgggcat tctggccgac gacgctgaac gtcaggcttc ccatcatcag
     1441 ggcggcaatc aggagtgcgc gcgtatgctt catctctcga cctatcggca cgaacgccga
     1501 tattccgaag gtgcgggttg aaccgggctg gaaacttagt gggctggaaa cgcgcggagg
     1561 ataatggaaa agaagcgctc gaatccgagg atgggaagag cgcgccgcag gatttgatag
     1621 gcggcgattc ccgcgtcatt ttcttcggcg cgtaacattt cgatcaggtt ggtcggacag
     1681 acctgcatgc gggtcggctt gatgctcaat ccggggggag cgagcgattc gatcctctgc
     1741 tggagcgttt ccagctcctg tttttcgtcg gtgatgagaa agagatcgag aggaagcgtc
     1801 acttcgggct cagtgctggc ttgcccgtcg atcgaggagt tttcccagtt aatgacgggg
     1861 atctcgatct ctttcagatg atcgagattt agaatgagag cgctctggag gagatctccg
     1921 ctggaggctt cgatcaggga gcggaccttg ctgatgacgt ctgccagtgg tgacccggac
     1981 atcataaact ggtgtgcccg ttgtgccgct cccagagcga gcaggttgcc tgtcatcgga
     2041 ttcgcggcgt tcagcgaaca cagcttggct tttccctggg gttgaatgcg cagcactccc
     2101 atacccccca gttggtgggt gcggttgtac gccgttccgt acgggagctc gagacgttgt
     2161 gcaatctggt gaatggaaag cactccggcc ggctgatcga gaaaagtctc gactatctgg
     2221 atgtacagtt gcatgagagg caggattata ccttaccgga gttcacaaga caaaggaaaa
     2281 aacacggcaa acttctcgta agcaaaaaaa gtgcctgctt ttcttgacac aacttcaatc
     2341 tgctgttaca atgcctgacc acttatattg tttgcggttt tttagggatg ggcgcgaagt
     2401 tttgactctg ttttcaggat gggaagcggg gctattgatt gacaggccgg ccaggtattt
     2461 caacaaccag gaccgaaaga cgaagaaacc agggaatcga tccccttggg atagccgagg
     2521 gcgtacgaat ggcttcttct tgctcgcatt ttcccgactg tgcagaacga cgtgccgtga
     2581 tacggaaatc cggggctacg gccccattgg cagcccaact gaggtaagga gttcgaaatg
     2641 gctttgacgc aatcgactgg aactgatcgt gtcttcatgg ctgacaaacg ggtgtttcgg
     2701 gatatcccga acctcattga aattcagact cagtccttcg aatggttcct gcaaccagga
     2761 aaaccattgt ctgagcgcaa gaagcaaggg ctgcaggaag tctttcacga aatcttccct
     2821 attcaggatt acgtcggaaa cctgtgcctc gaattcattg attattcgct cggtctcggc
     2881 atgtgcaaga attgtccgca gatcaagctg aataagaagt gcttgtacga tgattgcagc
     2941 tttcccgaaa aagagtgcga aatcgccttc gtgggcgaca acccgtttcg ggtcaagcac
     3001 agccctgatc agtgtcgcaa gcgcgatttg acctattcga ttccgctgaa catcaaggtt
     3061 cagctgatca tgcagcagac gggcgaagtc aaagaacagg aaatcttcct ggtcaacctg
     3121 ccgtgcatga ccgagcgcgg cacgttcatc atcaacggtg ccgagcgcgt cgtggtcagc
     3181 cagctgcacc gctctcccgg cgtctatttc agctacaacc gcccc
//
LOCUS       DAORHD010000476         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33971_length_3222_cov_4.131670, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000476 DAORHD010000000
VERSION     DAORHD010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33971_length_3222_cov_4.131670"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PKO06_10730"
     CDS             complement(<1..361)
                     /locus_tag="PKO06_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70163.1"
                     /translation="MQLMQTNRQLKWLVWALAALSAIFCVGSRVGAEQGTTGRDIRIK
                     VAEYPSELECTCAEGGIWKVGEQQGTLVPGARCRLVGRMDREADKTYHVVVESAPLRD
                     PARVEAAQQKWANGPRPL"
     gene            complement(402..1328)
                     /gene="murQ"
                     /locus_tag="PKO06_10735"
     CDS             complement(402..1328)
                     /gene="murQ"
                     /locus_tag="PKO06_10735"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014916237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNV70164.1"
                     /translation="MSETKSTFTGARPLTENRNPATTLIDRLPLASVVDLFVDQDRKV
                     AEAVSQCRDQIARAVEIIVGRLQKGGRLVYLGAGTSGRLGILDASECPPTFGVPAELV
                     VGLIAGGEIAVTHAVEGAEDDGDAAVADLKKIALGANDTLVGVSASGVTPYVRAGLAH
                     ARSVGGGAILLTCNPHGPFPETDVLLNPVVGPEVITGSTRLKSGTACKMVLNMLSSIS
                     MIRLGKVYQNLMVDVKATNRKLRWRSVQIVAEGAGIPFETAEQILSTCDGEVKTAIVA
                     SRRKLSPADARAEITRCHGILYKALGESETGQ"
     gene            1552..2499
                     /locus_tag="PKO06_10740"
     CDS             1552..2499
                     /locus_tag="PKO06_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HNV70165.1"
                     /translation="MSFLEPLRAIPGPTFLVVNLVYILIMFFVCWLYVRRDGTEDLNQ
                     PRLSAFSATEIAYLRGDVKTAAMTGLYKLMRLKLIKERREEPKGWIASWFASRRDGVL
                     FTDPDRPRPPLDKVELALLKYFEAPREAGKLFEEGTLSARVEEALAWTKVEFLNHKLF
                     RTEDQIVEQWMKAVGGTLVILLPGLLKLCLGIWNNKPVDLLVVELAVSGFVFFTWCDP
                     ASKRTNLGRAYLTRLEEHFAWMKDAFLTDSRQQRGSLTPDPALTLAIFGITALTSASA
                     FADFAQMYPRDPSSGGGCGGGCGGGCGGGCGGGCGGCGG"
     gene            2496..>3222
                     /locus_tag="PKO06_10745"
     CDS             2496..>3222
                     /locus_tag="PKO06_10745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70166.1"
                     /translation="MISRLVHWFTPATPRHTVPRFHLQWHLTNACNLHCRHCYQDHQP
                     EPDITFETVRATLDQAFDLLAFLRRVRQRPDLKLRLTLTGGEPLLSPVLFPLLELLST
                     QCSANDVECALLTNGTLIDRDCAHRLARYQPVYVQVSLDGPEALHEDMRGRGSFAPAV
                     AGLQHLRQAGVTTSISFTAHAGNYRSFPEVARLGRELQVDRVWADRLIPCGRGHELAT
                     ALMTPTQTEEFLHLMRQARSDAER"
BASE COUNT          602 a         1042 c          947 g          631 t
ORIGIN      
        1 gcagcggccg cggtccgttc gcccatttct gctgcgcggc ttcgacccgc gccgggtcgc
       61 gcaacggcgc ggattcgacg acgacgtgat acgttttgtc cgcttctcgg tccatgcggc
      121 cgaccaggcg acatcgagcg ccgggcacca gtgttccctg ctgctcgccg accttccaga
      181 tcccgccttc ggcgcacgtg cattcgagtt cagacggata ctcggcgacc ttgatgcgga
      241 tatcgcggcc ggtagttccc tgctcagcgc ccactctcga gccgacacag aaaattgccg
      301 acaacgcggc aagcgcccag accaaccact tcaactgcct gtttgtttgc atgagctgca
      361 cgatgcgcac acccctttca cacgtgggaa taatcgtgaa atcactgccc agtctcggat
      421 tcgcccaatg ctttgtacaa gataccgtgg cagcgagtaa tctcagcacg agcatctgcg
      481 ggcgacagtt ttctgcgaga ggcgacgatc gcggtcttca cctctccgtc gcaggtcgag
      541 agaatctgtt ctgccgtctc gaacggaatg ccggcccctt cggccacgat ctgcaccgaa
      601 cgccagcgca acttgcggtt ggtcgccttc acgtcgacca tcaggttctg gtacaccttg
      661 cccagacgaa tcatcgagat cgacgacagc atgttcagca ccatcttgca ggccgtgccg
      721 cttttgaggc gggtcgaccc ggtgatcact tcggggccga ccaccggatt gagcagaaca
      781 tcggtctcgg gaaacggccc gtgtgggtta caggtgagca gaatcgcgcc gccaccgaca
      841 ctgcgggcat gcgccagccc tgcccgcacg tacggcgtga cccctgacgc cgacacgcca
      901 accagggtat cgttcgcgcc cagcgcgatc ttcttcagat cggccacggc ggcatcacca
      961 tcgtcttccg ccccctcgac cgcgtgtgtc acggcgattt caccaccggc gatcagaccg
     1021 accaccaact cggcgggaac cccgaacgtg ggcggacatt cggaggcatc aagaatgccc
     1081 aatcgacccg atgttccagc gcccagatat accagacgtc cgcctttctg cagacgcccg
     1141 acgatgatct caacggcacg ggcgatctgg tcacggcact gcgaaaccgc ttcggcgact
     1201 ttgcgatcct gatcgacaaa aagatcgacg accgatgcga gcggcagccg gtcgatcaag
     1261 gtcgttgccg gattgcgatt ttcggtcagg gggcgtgccc cggtgaatgt cgatttcgtc
     1321 tcgctcacag aagacctcca gaggcagata cgcggcgctc ccgcgcgggt attgtaagct
     1381 tctcgctcac gattgccaag aggaaaactc gtgtcgctcc tgccagggga aaaaagccag
     1441 acgagagcat gaaatggccc tgacgtctga cagcgagggt tgcggctgtg gaagagaggg
     1501 agtagactgg gtgtgccggt tttcgacaat cacaacacaa ggatctcgag cgtgtcattt
     1561 cttgaaccac tgcgagcgat tcccggcccc acttttctcg tcgtgaacct ggtctacatt
     1621 ctgatcatgt tcttcgtctg ctggctgtat gtccgccgag atggcaccga ggacctcaac
     1681 cagccgaggc tgtcggcgtt ttcggcgacc gagatcgcct atttgcgcgg tgatgtgaaa
     1741 acggcagcca tgaccggttt gtacaagttg atgcgattga aactgatcaa ggagagacgc
     1801 gaagagccca agggctggat cgcctcgtgg ttcgccagtc gccgcgacgg cgtgctgttc
     1861 acggacccgg accgtccccg cccgccgctc gacaaagtcg aactggctct gctgaagtat
     1921 ttcgaggccc cgcgcgaagc cggcaaactc ttcgaggaag gcacactgtc agcccgtgtg
     1981 gaagaagccc tggcctggac aaaagtcgag ttcctcaatc acaagctgtt tcgcacggaa
     2041 gatcagatcg tcgagcagtg gatgaaagca gtcggcggca ctctggtcat tctgctcccc
     2101 ggacttctca agctctgtct cggaatctgg aacaacaaac ccgtcgatct gctcgtcgtc
     2161 gaactggccg tctccgggtt cgtgtttttc acctggtgcg accccgcctc gaaacgtacg
     2221 aatctcggtc gggcgtatct cacccgactt gaagaacact tcgcctggat gaaggacgcg
     2281 tttctcaccg actcgcgcca gcaacgcggc tcactcacgc ctgacccggc gttgactctg
     2341 gcgatcttcg gtatcaccgc cctgacgtcc gcatcggcat ttgcggattt cgcgcagatg
     2401 tatccccgcg acccctcgtc gggtggaggc tgtggtggcg ggtgcggcgg tggatgtggc
     2461 ggcggctgcg gtggtggctg cggagggtgc ggcggatgat ctcacgtctg gttcactggt
     2521 tcactcctgc gaccccgcga cacacggtgc cgcgttttca tctgcagtgg cacctgacga
     2581 acgcctgcaa cctgcactgc cgccactgct accaggacca tcaacccgaa ccggacatca
     2641 cgttcgagac cgtccgcgcc acactcgatc aggccttcga ccttctggcg tttctgcgcc
     2701 gggtccggca gcgaccggat ctcaagctcc gtttgacgct gaccgggggc gagccgcttc
     2761 tttctcctgt cttgtttccc ctcctcgagc tgctctcgac gcagtgttcc gcgaacgacg
     2821 tcgagtgcgc cctgctgacg aacggcaccc tgatcgacag ggattgcgcc caccgtctcg
     2881 cccgttatca gccggtctac gtgcaagtca gcctggacgg accggaagcg ctgcacgagg
     2941 acatgagggg ccgcggaagt tttgcgccag cggtcgccgg cctgcagcat ctgcgacagg
     3001 ctggagtcac gacctcgatc tcgttcacgg cccacgccgg caactatcgc tcctttcccg
     3061 aagttgcacg tctgggtcga gagctgcagg tcgatcgcgt ctgggccgat cgcctgattc
     3121 cctgtggccg ggggcatgag ctcgccaccg cgctcatgac gccgacgcaa acggaagagt
     3181 ttctgcacct gatgcgacag gcacgaagcg atgccgaacg cg
//
LOCUS       DAORHD010000477         3222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_33975_length_3222_cov_3.503315, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000477 DAORHD010000000
VERSION     DAORHD010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33975_length_3222_cov_3.503315"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKO06_10750"
     CDS             <1..314
                     /locus_tag="PKO06_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70167.1"
                     /translation="GNKMEMPKTRMEHLAKVTVEATAASAAAPVNADTKQGEEEVDVA
                     GAKIKCKTVETNITQGESVITSKIWSSDDVPGTLVKSVNNMEKPMKTTTTLTLIERKT
                     P"
     gene            complement(373..2148)
                     /locus_tag="PKO06_10755"
     CDS             complement(373..2148)
                     /locus_tag="PKO06_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HNV70168.1"
                     /translation="MPTDLREQILYQVVVTRFADGDTGNNFYNRKAIVRGDPHWRGDL
                     KGLAAQFPYFRDLGITALVVTSPLESRGSFDFEGDTPYDWTTVDPRLESPGFTYRDFI
                     RTAHEQGLRIIQEVPINASSFYGIRHQVFIDRLPIKYYLKKGETIPWPYELNWGDYRR
                     PFREDNDNPLAPLPFQDKVIADPFGRIGMVDQLTLVHVPSDGYDASRFFGTDLTKLPT
                     DWYHRDGFLSASEVDLWPVTQRKSLHSEAIDLVTENETVRRYLSRALTAPIKWGVDGF
                     LFPHAKHVSRDDLLPLCSYVWEQNPRLWLLADVGTIGTGFGELSNEPASARLKPWWYS
                     RTGHDPRQPDSGNDSGLAVSDYALFTKLLDTTTQGHFTGLGNLLAMDWAYGDPTRLIT
                     FLQNRHAGPSAVHAHRFSGTSVQAALTYNLLWMTRGVPCLLYGEEAEFANGGLLSLPQ
                     TGIALSTTGKAYFGDRLASDTVVTIKQGVLFRHLQRLNQIRKAVPALQTGLVSKGQEW
                     GIGMSFVREDPQKSSLAVIGLAARTAQEITVSGIPNGVYLDAITGGVASISSNTISFT
                     VKENSLGVWIRNGPGLIGTATEYLR"
     gene            2300..>3222
                     /locus_tag="PKO06_10760"
     CDS             2300..>3222
                     /locus_tag="PKO06_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70169.1"
                     /translation="MTTILLSIAALAAVALALTWYVRQSRSANLWNERRLLLLSSQAK
                     PQAQAATWIDAMSQDGERFWSDAELVAAAIRILIRLARFQQAKLLHGKVRGRYGTLEW
                     HKDTEADLIVGAGSSTDADFKKLEASCLRKPEQSVWKERYIELCQKHVPVEPKAVDLL
                     NDWYIADRDQRLISVLAESYLRRKQYDATSEAFFLQRLETDGNNPRLLQALAACREKD
                     RDPVRAEEYRQAAASFEGADGVRVTKTPLATPNPTTRGQTSDQAGQKFLAAIRATREG
                     QTPAPRTVSPPPATSRPAIAPPASPRPAIPPP"
BASE COUNT          731 a          879 c          983 g          629 t
ORIGIN      
        1 ccggcaacaa gatggaaatg cccaaaaccc gcatggaaca cctggccaag gtgaccgtgg
       61 aggcgaccgc ggcttcggcg gctgccccgg tgaatgccga taccaagcag ggtgaggaag
      121 aagtcgacgt ggctggtgcc aagatcaagt gcaagaccgt ggaaaccaat atcacccagg
      181 gtgaatcggt catcaccagc aagatctgga gcagtgacga tgtgccggga acgctggtca
      241 agtcggtgaa caacatggag aagccgatga aaaccacgac cacgctgacc ctgatcgaga
      301 gaaagactcc ctgagtttca ggcaggaaaa aagccgacct gcgggtcggc ttttttcttt
      361 tgtccaggtt ggttagcgca aatactcggt ggctgtgcca atgagacctg gaccatttct
      421 tatccagacg ccaagagagt tttccttgac cgtgaatgaa atggtgttgc tgctgatcga
      481 ggccacgcct cccgtgatgg catcgagata gacgccgttg ggaattcccg agacggtgat
      541 ttcctgcgcg gtgcgcgcgg ccagaccgat cacggccagg ctcgacttct gagggtcctc
      601 gcggacaaaa ctcatcccga taccccattc ctgccccttg gaaacgagcc ctgtctgcag
      661 ggcgggaacg gccttgcgga tctgattcaa tcgctgcagg tgacgaaaga gaacaccctg
      721 tttgatcgtg acaacggtgt cagaagcgag tcggtcacca aaataggctt tgccggtcgt
      781 ggataacgcg atccccgttt gtgggaggct gagcaggccg ccattggcga attccgcttc
      841 ctcaccatac aaaagacagg gcactccgcg cgtcatccag agcagattgt aggtcagagc
      901 cgcctgtacg gaagtgccgg aaaaacgatg tgcgtggacg gctgacggcc cggcatgacg
      961 attctgcaga aaagtgatca ggcgggtcgg gtctccgtat gcccaatcca tggccagcag
     1021 attcccgaga ccggtaaagt gcccctgcgt ggtcgtgtcg agaagtttcg tgaacaaggc
     1081 atagtcagaa acagccagcc cggaatcgtt tccagaatcg ggttgacgtg gatcatgacc
     1141 ggtacgtgaa taccaccacg gcttaagacg ggctgacgca ggctcgttgg acaactcacc
     1201 aaagccggtg ccgatggttc ccacgtcggc gagcagccac aagcgagggt tctgttccca
     1261 cacgtaactg cacagtggaa gcagatcgtc acgtgagacg tgtttcgcgt gcggaaacag
     1321 aaacccatcg acgccccact tgattggcgc tgtcagtgct cgtgagagat agcggcgcac
     1381 cgtttcgttc tcggttacca gatcgatggc ttcggaatgg agcgacttgc gctgggtgac
     1441 cggccagaga tcgacttcgg atgccgaaag aaagccgtcg cgatggtacc agtcggttgg
     1501 cagcttcgtg aggtcagtgc cgaagaaacg cgaggcatcg taaccatcac tgggcacgtg
     1561 aaccagggtg agctgatcga ccatgccgat gcggccgaat ggatcggcga tgactttgtc
     1621 ctgaaacggc agcggagcca gcgggttgtc gttatcttcg cgaaacggcc ggcggtagtc
     1681 tccccaattc agttcatacg gccagggaat cgtctcgcct ttcttcaggt agtatttgat
     1741 tggcaggcga tcgataaaga cctgatgacg aatcccgtag aaactcgagg cattgatcgg
     1801 gacttcctga atgattcgca acccttgttc gtgagcagtt ctgatgaaat cacgataggt
     1861 gaacccgggt gattcgagcc gcggatccac ggtggtccaa tcgtaggggg tatcgccttc
     1921 gaaatcgaag ctgccgcggc tttccagggg tgacgttacc accagggcgg tgattcccag
     1981 atcgcgaaag tagggaaact gagccgccag acctttcaaa tcccctctcc aatgcggatc
     2041 gcctcggaca atcgccttgc ggttatagaa attgttgccg gtgtcgccat cggcaaagcg
     2101 ggtgacaacc acctggtaaa gaatctgttc gcgaagatcg gtcggcatgg gccactcgga
     2161 ggccatgagc ggtggtgcgg aacagaaaac ggaaagggcc aggagaagaa gacgtgacag
     2221 tgctgaaaac atgaagtcta ttgtatcatg gtgagtggtt tttctatgct atagtcgtgt
     2281 ctgacgggtc ttattgggaa tgactaccat ccttctgtct atcgccgctc tggctgcggt
     2341 ggcgctggct ctgacctggt acgttcggca gtcgaggtcg gcgaacctct ggaatgagcg
     2401 ccgactgctt ttgctttcca gccaagccaa accgcaggct caggccgcaa cctggatcga
     2461 tgccatgtcg caggatggcg agcgtttctg gagtgacgcg gaactcgtgg cggccgcgat
     2521 tcgcatcttg atccgtctgg cgcggtttca gcaggcgaag ctgcttcacg gcaaggtgcg
     2581 cgggcggtat gggacgctcg agtggcacaa ggatacggaa gcggatttga ttgtcggcgc
     2641 gggatcttcg acggatgccg atttcaagaa gctcgaagct tcctgtctgc ggaaacccga
     2701 acagagtgtc tggaaagaac ggtacatcga gctgtgtcag aagcatgttc ccgtcgaacc
     2761 gaaagcggtg gacctgttga acgattggta catcgccgac agagatcagc gactgatctc
     2821 tgttcttgcc gaatcctacc tgcgacgaaa acagtatgac gcgacctccg aagcgttctt
     2881 tctgcagcga cttgagacgg acgggaacaa cccgcgactg ttgcaggcgc tggccgcgtg
     2941 tcgagaaaag gatcgtgatc cggtgagagc tgaagagtat cggcaggccg ccgcgagttt
     3001 cgaaggggct gatggggtgc gagtcacgaa aacgccgctg gcaacaccta acccgacgac
     3061 caggggacag acgtcagatc aagccggaca aaagttcctg gccgcgatcc gcgcgacccg
     3121 tgaaggtcaa actcctgcac cgcgaacggt ttccccgccc ccggcaactt ccaggcccgc
     3181 gatagccccc cccgcctccc cgcgccccgc catacctccc cc
//
LOCUS       DAORHD010000478         3220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34006_length_3220_cov_2.872354, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000478 DAORHD010000000
VERSION     DAORHD010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34006_length_3220_cov_2.872354"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="PKO06_10765"
     CDS             <1..1740
                     /locus_tag="PKO06_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70170.1"
                     /translation="WEKPAVYEKWNTYSRIRVLGDDRILTPAFGWGLSPTLPATVGAR
                     QLVVNIDANAGTVMTAFSGRPEDVGHLQYDISNLAHYLRSQADVFVVGVGGGRDILSA
                     IAFNQRRIVGLEVNRDMIDLVTRVFADLTGRLHERPDVRLINDEARSYLVRTNERFTI
                     IQVSLIDSWAATAAGAFVLSENTLYTTEAWQLFLQRLTSNGILSFSRWYRQNAPGEIQ
                     RLLALAVHALRCNGVSDPRRHLLLVRVSAPEHTGDPNNGVGTLLVSPSPFSDADLQKL
                     SDVCRALRFDIALSPTTCIDPRFLPILETPDLLTYAAAQPQNIAPPTDDRPFFFHLHR
                     IRDVFSTGGGETRVEVFLLALLGFITVLTALCVLLPLRWRGDVPSSPDQFWFSVYFSG
                     IGLGFMLIEMAFLQWLVVLLGHPTYSLSVVLFSLLLASGAGSWLTARFVAPDVPPGKF
                     WIGGFLMALVASGLAVPGVAAAQAGAENLSRMFTAVLLILPLGTILGTAFPRGMALCQ
                     QRQPALAPWLWGTNGAMSIVGSILAVIIALEWGITAVFWTGTAGYLAAFVAIRRIASS
                     MDSEVTTRAQAIE"
     gene            complement(1747..3054)
                     /locus_tag="PKO06_10770"
     CDS             complement(1747..3054)
                     /locus_tag="PKO06_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNV70171.1"
                     /translation="MATMTEYLAYFQLSESFTLEQLKKAYYAQAQLLHPDRFSSPEEK
                     KAASDEFQKLQDAYTNLQVLLEERRKSEEAFLAAKRNLQIAKEERVSRQTNRVLDCET
                     SRTLMYNLFGMSVQALSLYLSLLNGMYEDDMADLYQPIWDFPEHYEGDTDEKKLWHIS
                     WQDHVRRACLHRDPKKRATFWVDRSPEYREAQKAVRVMPAPFLGRLLALEVAAFDLYW
                     PLPEDRGHRFQLFTIEDEFLKRCYHQLFEPLGISAETVYRYRGSLESVFKTLYPEWKT
                     LHHLVFPRGDGTIFGAALSAERPLTLMADTRSKCATVAPEAVAMVGAGMRLCQGDDPR
                     CVVIGAGTALLMPVCGEGSMYNGNWSMAEVVMSLAKLEVFTREAILSEPAYRFCRDHL
                     VQLLEGFSEHLATYATTVPDAEAEKLGLIRSWVHCLRADLAKD"
BASE COUNT          609 a         1013 c          894 g          704 t
ORIGIN      
        1 tgggagaaac ccgcggtcta cgagaagtgg aatacctatt cgcgcatccg ggttctgggc
       61 gacgatcgca tcctgacccc cgccttcggc tggggcttga gtccgacctt gcccgccacc
      121 gttggcgcac ggcaactggt ggtcaacatc gacgcgaatg cagggaccgt gatgacggca
      181 ttttccggca gacccgaaga cgtcggccat ctgcaatacg atatctcgaa tctggcgcac
      241 tatctacgtt cgcaggcgga cgtgttcgtt gtcggtgtcg gcggaggtcg cgacattctt
      301 tccgcaatcg ccttcaacca gcggcgcatc gtcggtctcg aagtaaaccg cgacatgatc
      361 gacctcgtca cccgcgtgtt tgccgatctc accggtcgac ttcacgagcg acctgacgtg
      421 cggctgatca acgatgaggc gcgcagttac ctggtacgaa ccaacgagcg cttcaccatc
      481 atccaggtct cattgatcga ttcatgggcc gcaaccgctg ccggagcctt cgttctcagc
      541 gaaaacacct tgtacactac cgaagcctgg caacttttcc tgcaacgact gacatcgaac
      601 gggattctct cgttctcgcg ctggtaccga cagaatgccc cgggtgaaat acagcgcctg
      661 ctcgcgctgg ccgtgcatgc cctgcgctgc aacggcgttt ccgatcctcg gcgtcatctg
      721 cttctcgtgc gggtttcagc ccccgagcac actggcgacc cgaacaatgg cgtcggaacc
      781 ctgctggttt ccccctctcc gttcagcgat gccgacctgc agaaactttc cgatgtgtgc
      841 cgcgctctgc gcttcgacat cgcactgagt ccgaccacct gcatcgatcc ccggtttctc
      901 ccgattctcg aaacgcccga tctgctcacc tatgcggcgg ctcagccaca gaacatcgcc
      961 ccgcccaccg atgaccgtcc tttcttcttt cacctgcacc gcattcgcga cgtcttttcc
     1021 accggcggcg gcgagactcg tgtcgaggtt tttctgttgg ctctgctcgg cttcattacc
     1081 gtgctcaccg ctctgtgcgt gcttctgcca ctgcgctggc gcggggacgt tccgtcatcc
     1141 cctgatcaat tctggttctc cgtctatttt tccggcatcg gactcgggtt catgctcatc
     1201 gaaatggcgt ttctgcagtg gctggtcgtg ctgctcgggc acccgaccta cagtctctcg
     1261 gtcgtgctct tctcgctgct gctggcgagt ggtgccggca gctggctcac ggcccggttc
     1321 gttgcccctg acgtgccacc cggcaagttc tggatcggcg ggtttctcat ggctttggtg
     1381 gctagcggtc tggccgttcc gggagtagcg gccgctcagg cgggtgccga aaacctgagt
     1441 cgcatgttca ccgcggtgtt gctgattctg ccactgggaa cgatcctggg cacggccttt
     1501 ccgcgcggaa tggcgctctg tcagcaacga cagccggccc tggctccctg gctctggggc
     1561 accaacggcg ccatgtccat tgtcggttcg atccttgccg tgatcatcgc cctcgagtgg
     1621 ggcatcactg ccgtcttctg gaccggcact gccggctacc tcgcggcatt cgtggcaata
     1681 cgacggatcg cctcctccat ggacagcgaa gtcacgacgc gcgcacaagc catcgagtga
     1741 tcgatctcag tccttggcca ggtcagcgcg aaggcaatgc acccacgagc ggatcaggcc
     1801 aagcttctcc gcttccgcat cgggaaccgt ggtggcatac gtcgccagat gctcggaaaa
     1861 gccctcgagc agctgcacca gatgatcgcg acagaagcga taggccggct cggacaaaat
     1921 ggcctcacgg gtaaacacct cgagcttggc cagtgacatc accacttcgg ccatgctcca
     1981 gtttccattg tacatgctgc cttcgccaca aacgggcatg agaagagcgg ttccggctcc
     2041 gatcacgacg caccgaggat catcgccctg gcagagacgc attccggccc ccaccatggc
     2101 aaccgcttca ggagccaccg ttgcgcattt ggaacgcgta tcggccatca gggtgagagg
     2161 gcgttcggcg gaaagagccg ctccgaagat cgtcccatcg ccgcgtggga acacgaggtg
     2221 atgcaatgtt ttccactctg gatagagcgt cttaaagaca ctttcgaggc tgccgcgata
     2281 gcggtacacc gtttcggcac tgatgcccaa cggttcaaac aattgatgat agcagcgttt
     2341 gagaaactca tcttcgatcg tgaacagctg aaaccggtgc ccacgatctt cgggtaacgg
     2401 ccagtacaag tcaaaagccg cgacttcgag ggccagcaaa cgtccgagga atggcgccgg
     2461 catcacgcgc acggctttct gcgcttcccg atattcaggc gagcggtcga cccagaaggt
     2521 ggctctcttt ttcgggtctc gatgcaggca ggctcgtcgc acgtgatcct gccaggagat
     2581 gtgccagagc ttcttttcat cggtgtcgcc ctcataatgc tccgggaaat cccagatggg
     2641 ctggtagagg tcagccatat catcttcgta cataccgttc agaagggaaa gatacagcga
     2701 caatgcctga acactcattc cgaacagatt gtacatcaac gttcgactgg tctcacaatc
     2761 gagaacccgg ttcgtttgcc gactgacgcg ttcttccttg gcgatctgca ggtttcgctt
     2821 ggccgcgaga aacgcctctt cagatttgcg gcgctcttcg agcagcacct gcaggttggt
     2881 gtaggcatcc tgcaacttct gaaactcgtc acttgcggct ttcttctctt ccgggctgct
     2941 gaagcgatcg gggtgcagca actgcgcctg agcatagtaa gccttcttca actgttcgag
     3001 cgtaaacgac tcggacaatt gaaaatacgc caggtattcc gtcatcgtcg ccatgtcggc
     3061 aggcctctca cgactagagt ttcacctatg gtagcccccg atggcagcca tgacaagaaa
     3121 tttctcgcga gagacgggta ccggtagttc agcctcttcc gtgccgtgcc atcaagaaac
     3181 ggcgcaggct aatgaggcga aaatcgtaga caattctgtc
//
LOCUS       DAORHD010000479         3214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34090_length_3214_cov_2.888572, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000479 DAORHD010000000
VERSION     DAORHD010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34090_length_3214_cov_2.888572"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKO06_10775"
     CDS             <1..366
                     /locus_tag="PKO06_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70172.1"
                     /translation="YMQPMMDAGFKFSGDYAWANNTKYIPQLGRGHPKFSVQPGDYIS
                     NGTHVMTVLKVNGDKVTVVSGNAGGGPENGAGTVRIEEMSCSKIEVVKKHSDFTLETL
                     QSHANDEAWLKAHGITRKE"
     gene            378..1256
                     /gene="nadC"
                     /locus_tag="PKO06_10780"
     CDS             378..1256
                     /gene="nadC"
                     /locus_tag="PKO06_10780"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNV70173.1"
                     /translation="MIRTFVQHCFDEDLGPACRDVSVHALSEAALRPAIGLVRAKASG
                     LFCGGFLFALVVQEATDRFSFLIGRPAAPIIVSDLIDDGSELKENSIIARLSGPTGAL
                     LMAERILLNLLQRLSGVATLTRQYVDQVKHTSCRILDTRKTTPGLRALEKRAVQVGGG
                     VNHRQGLYDMVMLKDNHITAMGGDVSRAVLQARQQIGPTLRIEVETSSLAQVQAAVEA
                     GANVVMLDNMSPQLMRECVAWVNGCVPVEASGGITLENVRAVAETGVDYISVGALTHS
                     APALDISMKITVGDRS"
     gene            complement(1267..1722)
                     /gene="mobB"
                     /locus_tag="PKO06_10785"
     CDS             complement(1267..1722)
                     /gene="mobB"
                     /locus_tag="PKO06_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNV70174.1"
                     /translation="MEKLPPIIRVLGRSGSGKTTLIETLIRELQPLRIGVAKHTRHHL
                     ELPANTKDTTRYAAAGAVVSSGLSPADAEMFVRVPWNWDDVLAMMTKHVDLILVEGAR
                     DLDLPTILVGEAPEGACLKNVLTCLPRYPVLTPEVIRVVGRFIQNVRPE"
     gene            complement(1725..2717)
                     /gene="moaC"
                     /locus_tag="PKO06_10790"
     CDS             complement(1725..2717)
                     /gene="moaC"
                     /locus_tag="PKO06_10790"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNV70175.1"
                     /translation="MADSSSLTHLDAAGRARMVDVGEKPLTRRRAVAFGAMWVGTTTL
                     RALLEGRIAKGDAWAAARLAGIAGAKKTADLIPLTHPLRLDAVRVHLSAWGAERVVVF
                     AEAMATDRTGVEMEAMTAATVALLTLYDMTKAVSRGARIEEVCLLAKEGGKSGDWLAE
                     GVERGTIAKLALSTGKGTPKTPCDEALLVPGFGLEGDAHGGDWHRQVSLLGVESIRKM
                     QQMGLKVDFGSFAENVATSGVCLYELPLGSLMLTSQGAILEVTQIGKECHSRCAVYHT
                     AGDCVMPREGIFIRVRLGSPIRTGDSLLVKRLPTGYHWTKADLDQRSQDGKNLT"
     gene            complement(2730..>3214)
                     /locus_tag="PKO06_10795"
     CDS             complement(2730..>3214)
                     /locus_tag="PKO06_10795"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV70176.1"
                     /translation="STRITHTDPRVVGCAIVLATVVRLGLEGADPNPETVVTAVLEKA
                     QVYAAELMRRLKLVKDLLRVELLAAVQQIGNGGFCLESVPLALHCFLRYPRKFDEMIV
                     AAANVGGDSDAIAAMAGAMFGAFHGIGAISSRWLDRLENVEKLKQTGADLYRMTVPNR
                     "
BASE COUNT          678 a         1005 c          901 g          630 t
ORIGIN      
        1 tatatgcagc cgatgatgga tgccggattc aagttctcgg gggactatgc ctgggccaac
       61 aacacgaagt acattccaca attgggtcgc ggacatccga agttttccgt gcaacccggg
      121 gattacatct cgaacggtac gcatgtcatg acggtgctca aagtgaacgg agacaaggtg
      181 acggtcgtct ccggcaatgc tggcggtggc cccgaaaacg gagccggcac cgtgcgtatc
      241 gaagaaatgt cctgcagtaa gatcgaagtg gtgaagaagc acagcgactt cacgctcgaa
      301 actctgcaga gccatgccaa cgatgaagcc tggctgaaag cacatggcat cacccggaag
      361 gaataggagg gaagttcatg attcgcacgt ttgtacaaca ctgtttcgat gaagatctcg
      421 gccctgcctg ccgtgatgtc tcggttcatg cgctgagcga agccgcactt cgcccggcga
      481 tcggtctggt tcgcgccaaa gccagcggtc ttttctgtgg cggctttctg tttgctctgg
      541 tggtccagga agcgacggac cgtttttcgt ttctgatcgg cagacccgcg gctcccatca
      601 tcgtttcaga tctcatcgac gacgggtccg agctgaagga aaactccatc atagctcggc
      661 tttccggacc gaccggtgcc ttgctcatgg ccgaacgcat tctgctcaac ctgctgcagc
      721 gtctttcggg agtggcgact ttgacccggc agtatgtcga tcaagtcaaa cacacctcat
      781 gtcgcatcct ggacactcgc aagacaaccc cggggcttcg cgccctcgag aaacgtgccg
      841 tacaagtcgg tggaggcgta aatcaccgtc aggggctgta tgacatggtc atgctgaaag
      901 acaatcacat caccgccatg ggtggcgatg tcagccgtgc cgttctccag gcacggcaac
      961 agatcggtcc caccctccgc atcgaagtcg agacctcctc gctggcacaa gtgcaagcag
     1021 ctgtcgaggc cggggcgaat gtcgtcatgc tcgataacat gagcccgcag ctgatgcgcg
     1081 agtgcgtggc ctgggtgaat ggctgcgttc cggtcgaagc ttctggcggt atcacgctcg
     1141 aaaatgtacg cgccgtcgcg gaaaccggag tcgattacat ttcggtcgga gccttgacgc
     1201 attcagcccc ggctctggat atctcgatga aaatcacagt cggcgacaga agctgaagtt
     1261 cgtccgtcac tccggtcgga cgttctggat gaagcggccg acgaccctga tcacttccgg
     1321 agtcaacacc gggtaacgcg gcaggcaggt cagcacgttc ttcaggcatg ccccctcggg
     1381 ggcttcaccg accagaatcg tcggcaaatc gagatcgcga gcgccttcga ccagtatcag
     1441 atcgacatgt ttcgtcatca ttgccaatac atcatcccag ttccatggaa ctcggacaaa
     1501 catctcggca tccgctggcg agagtccact cgacacgacg gcaccggccg cggcatagcg
     1561 ggtggtatcc ttggtattcg cgggcaactc gagatggtgg cgggtatgct tggccacgcc
     1621 aatacgcagg ggctgcagtt cacggatcag cgtctcaatc agggtcgttt ttcctgaccc
     1681 cgaacgaccg agaacgcgaa tgatcggcgg gagcttctcc atgttcaggt cagatttttg
     1741 ccgtcctgcg aacgttggtc gagatccgct ttcgtccagt gatacccggt cggcagacgc
     1801 ttgaccagaa gagagtcgcc ggtccggatg ggggatccga ggcgcacgcg aatgaaaatt
     1861 ccctcccggg gcatcacaca gtctcctgcc gtgtggtaga cggcacaccg cgagtggcac
     1921 tccttcccga tctgtgtcac ttcgaggatg gctccctggc tggtgagcat caacgagccc
     1981 aagggcaact cgtacaggca aacacccgag gtcgccacgt tctcggcaaa agacccaaaa
     2041 tccactttca gccccatctg ctgcattttt ctgatactct caaccccgag caacgacacc
     2101 tgacgatgcc agtcgccgcc atgcgcgtct ccttcgagac caaaaccagg aaccagcaaa
     2161 gcctcatcac atggtgtttt cggcgttccc ttcccggtac tcagcgcgag tttagcgatc
     2221 gtgccgcgct cgaccccttc ggccagccaa tctccactct tccccccttc tttcgccaac
     2281 agacagacct cttcgatccg agcgccgcga ctgactgcct tggtcatatc gtacagagtc
     2341 agcaatgcga cggtcgccgc cgtcatcgct tccatctcga cgccggttcg atccgtggcc
     2401 atggcttcgg cgaagaccac aacccgctcg gcaccccagg cgctgagatg cacgcgcacg
     2461 gcatccagtc gtaacggatg cgtgagcgga ataagatcag cagtcttctt ggcaccagcg
     2521 atgcccgcca gtcgcgccgc tgcccaggca tcgcccttgg cgatacgacc ctcgagcagg
     2581 gcacgcaggg tcgtggttcc cacccacatc gcaccgaagg caaccgctcg ccgccgggtc
     2641 aagggtttct cgccgacatc gaccatgcga gcacggccag cggcatcgag atgcgtcaac
     2701 gaagacgaat cagccatgac ggcatctcct cagcggttgg gaaccgtcat acgatacaga
     2761 tcggcaccgg tctgcttgag tttttccacg ttttcgagcc gatccagcca acggctcgaa
     2821 atggccccga taccgtggaa cgcaccaaac atcgcccccg ccatggcagc gatggcatcg
     2881 gaatcgcccc caacgttggc cgcggcgacg atcatctcgt cgaatttgcg gggataccgc
     2941 agaaagcagt gcagggcgag cggaactgat tcgaggcaga atccgccatt cccgatctgc
     3001 tgaacagccg cgagcagttc gacgcgcagc agatctttca ccagtttcaa gcgtcgcatc
     3061 agttctgcgg catacacctg tgccttttcc aggaccgccg tgacgacggt ttccggattc
     3121 gggtccgctc cctcaagccc cagccgcacg accgtggcaa gcacaatggc acaaccgacc
     3181 actcgcggat cggtatgcgt gatccgcgtt gact
//
LOCUS       DAORHD010000480         3213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34094_length_3213_cov_4.217543, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000480 DAORHD010000000
VERSION     DAORHD010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34094_length_3213_cov_4.217543"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1258
                     /locus_tag="PKO06_10800"
     CDS             74..1258
                     /locus_tag="PKO06_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70177.1"
                     /translation="MIVGQGVCRSLERIGVPIASFVDRSSSLQGKRALGYEIDKPENI
                     FQTVKEGKGIIIATSGHCDLEIGKCCEEAGFVSGRDFILCRELNDVDPSVEVAGMCNL
                     HCISCPRGNESNHPPAGFMTPETYRKVLDKLLREIPLLGSIQLYTWGEPLLNPRLPEI
                     IACTREARVLSAISSNLNFARNLDQVIAAKPDWFKVSCSGWENSYEITHTGGTWSMFL
                     KNLHHLAECRRLYHPEMQITVNYHLYKNNLGEDYRRMEDLCRELDLIFRPSPAYLYPA
                     DAIMEYVDGKPLSEAANRTIPLLMMGLDEGMAKARERASQPCPEERCFPINWDCKVRA
                     CGVYYRPFIAENFLETPLAEIMERKRTSQLCRECKKRGIHQFTGVYLAEKRVERPSGS
                     KT"
     gene            1255..2505
                     /locus_tag="PKO06_10805"
     CDS             1255..2505
                     /locus_tag="PKO06_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70178.1"
                     /translation="MKQPIDILRERSAEGRQILIYGAGSQGRGVLRALRRNAIDVAGF
                     IDRNPSVQGNNVGDLTVHSPDLLRRPASRDHYFIIIAAFFFEREIASFLGGFGYEVGR
                     HFLAYSSLKPHDYAVEVSGVCNLRCISCPRADRRPTDRNAAMMSLDHFRQVIDKIRRE
                     SPFVGNLQLYQWGEPTLNPALPAMIRLAREQGFLCSLSSNLNHKADFRALIEALPECL
                     RLSVSGIGENYELTHTGGTWSTFLEHVREIGKLRLELNPQMKVELYYHQYRHSVGDQQ
                     GRIAELCRELQFEFHPVPAYIISLDDVLAYAEGTPLPPPACQARKLLLVDIDEGLQRA
                     KAEAALPCDALRVIMINADLSVSTCMMFYDPEGNTLVQNFLSTPLETIVERRKRAPLC
                     GRCMKHGIHRYCSVYAQLSEERRY"
     gene            2502..>3213
                     /locus_tag="PKO06_10810"
     CDS             2502..>3213
                     /locus_tag="PKO06_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70179.1"
                     /translation="MKHDVPRVMHLLPHMGGGVGSVVRSLLHREHHAVQAGFAHSIVA
                     LESLNVPSRSFLEEHHIPWIEQADRDTISRIIDTVDIVLIHWWNHPLLMQVLYDGLPP
                     CRLALWSHVNGYHVPQSFFPALFDLPDRFVFTTSASLQAPVVQRLPKTVKQHLRVIRS
                     CAGIPPGAERPVVKSPPFQAGYLGTVEPAKMHRDFLELCIKARLPTPCLVAGGPAHEE
                     LRESARQKGIAQHFDILGP"
BASE COUNT          695 a          925 c          837 g          756 t
ORIGIN      
        1 gtaaccctcc atgatgactg tagaagatgt tcgccgcttt tgctccggta aaaaggtctt
       61 tctgtggggc gccatgattg tcggccaggg agtctgccgc tctctggaac gaattggtgt
      121 tccgatcgct tcgttcgtgg accgttcttc ttcgttgcag ggaaaacgtg cacttgggta
      181 cgagatagat aaaccggaaa acatttttca gacagtgaaa gaaggcaagg gaatcatcat
      241 tgctacctcc gggcactgtg atctggagat cggaaaatgc tgcgaagagg ccggcttcgt
      301 cagtggacgc gacttcattc tgtgtcgcga attgaatgat gtcgatccca gtgtcgaagt
      361 ggcgggcatg tgcaaccttc attgcatttc ctgtccacgc ggcaatgaat cgaatcatcc
      421 tcctgccggg ttcatgactc cggagacgta tcggaaggtt ctggacaaac tgcttcgcga
      481 gatcccactc ctggggagca ttcaactcta tacctggggc gaaccgctct tgaatccgcg
      541 actgccagaa atcatcgcct gcactcgcga ggcccgggtt ctctccgcca tatcctccaa
      601 cctgaatttc gcccgcaacc tggatcaggt gatcgccgcc aaaccggact ggttcaaggt
      661 atcctgttcc ggctgggaaa acagttacga gattacgcat accgggggca cctggtccat
      721 gttcctgaag aatctgcatc atcttgcaga gtgccgtcgc ctgtatcatc ccgagatgca
      781 gatcaccgtg aattaccact tgtacaaaaa caaccttggc gaggactatc gccgtatgga
      841 agacctctgt cgggagctcg atctcatttt ccgccccagc ccggcgtatt tgtatcctgc
      901 tgacgccatc atggaatacg ttgacggcaa acccctttcg gaggcagcaa atcgaacaat
      961 ccctcttctc atgatgggat tagacgaagg aatggccaag gctcgcgagc gagcttcgca
     1021 accatgcccc gaggaacggt gcttccccat caattgggac tgtaaggttc gtgcgtgtgg
     1081 tgtgtattat cgaccgttca ttgctgagaa ctttctcgaa acgccgctgg cggaaatcat
     1141 ggagcgcaaa cgtactagtc agttgtgtcg cgaatgcaag aaacgaggta ttcaccagtt
     1201 taccggagtg tatctggcag aaaagagggt cgaacgaccc tcgggttcga agacgtgaaa
     1261 cagccgatag atattctcag agagagatct gccgaaggcc ggcagattct catctatgga
     1321 gcgggaagtc agggccgtgg cgtgttgcgc gcccttcgcc gcaacgcgat cgatgtcgcc
     1381 gggttcatcg atcgaaatcc ctccgtacaa ggcaacaacg ttggcgacct gacggtacac
     1441 tcaccggatc tccttcgccg tcctgcaagt cgagatcact atttcatcat catcgccgct
     1501 ttcttttttg agcgcgaaat cgcttcgttt ctcggcggct tcggctacga agtcggcaga
     1561 cactttctcg cctacagcag tctaaaacct catgactatg cggttgaagt atccggagtc
     1621 tgcaaccttc gctgcatctc ctgcccacgg gctgaccggc gtcccaccga ccgcaatgcc
     1681 gcaatgatga gtcttgatca ctttcgccag gtcatagaca agattcgccg cgagtcgccg
     1741 tttgtcggga atctgcaact gtaccaatgg ggagaaccga cactgaatcc cgcactgccc
     1801 gccatgattc gactggcacg cgagcaaggc tttctgtgtt ctttgtcgag caacctgaat
     1861 cacaaagccg actttcgcgc tctcatcgag gcacttccgg aatgtctcag actatccgtg
     1921 tccggaattg gtgagaacta tgagttgacg cacacgggcg gaacatggtc gacttttctt
     1981 gaacatgttc gcgagattgg taagttgcgg ctcgaattga atccccagat gaaagtcgag
     2041 ttgtactatc atcagtatcg tcacagtgta ggcgatcaac aaggtcgcat cgctgaactc
     2101 tgtcgagaac tgcagttcga gttccatccc gtgcccgcct acatcatctc cctggatgat
     2161 gtattagcct atgccgaggg aaccccgttg cccccgcccg cctgccaagc acgcaaactg
     2221 cttttggtcg atatagacga ggggttgcag cgagccaagg ccgaggcagc tcttccttgt
     2281 gatgccctgc gtgttatcat gattaacgct gatctatcgg tgtcgacctg catgatgttc
     2341 tacgatcccg agggcaatac tctggtacag aactttttga gcaccccgct cgaaaccatc
     2401 gttgagcgtc gcaaacgagc tcctctctgc ggtcgctgca tgaaacacgg tattcaccgc
     2461 tactgtagtg tctacgctca actcagcgag gagagacgct attgaaacac gatgtccctc
     2521 gcgtcatgca cctgttgccg cacatgggcg gcggggtggg gtcggtggtg cgttccctgt
     2581 tgcatcggga acaccacgcg gtccaagccg gattcgctca ctccatcgtc gccctcgagt
     2641 cgctcaacgt gccctctcgc tcatttctgg aggagcacca cattccctgg atcgagcaag
     2701 ccgaccggga cacgatcagt cgaatcatcg acacggtcga catcgttctc atccattggt
     2761 ggaatcatcc ccttctcatg caagtgcttt atgatggtct gccgccctgt cgtctcgccc
     2821 tctggtccca cgtgaacggg tatcatgtac cgcaatcatt ttttccggca ctattcgacc
     2881 ttcctgaccg ttttgttttc acgacatcag cttcacttca ggctccggtc gttcaacgcc
     2941 tgccgaagac ggtgaagcag cacctccggg tcatccgcag ttgtgcaggg attcccccag
     3001 gtgccgaacg ccccgtcgtc aaatcacccc cgtttcaggc tgggtatctt ggcaccgtgg
     3061 aaccggcaaa gatgcatcga gactttctcg aactttgcat caaagctcga ctccccactc
     3121 catgcctcgt tgccggcggg ccggcacatg aagaactccg cgaaagtgct cgacaaaagg
     3181 gaattgccca gcatttcgat attctcggcc ccg
//
LOCUS       DAORHD010000481         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34160_length_3209_cov_3.668041, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000481 DAORHD010000000
VERSION     DAORHD010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34160_length_3209_cov_3.668041"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="PKO06_10815"
     CDS             complement(<1..1314)
                     /locus_tag="PKO06_10815"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70180.1"
                     /translation="MKTRKILLIEPPMRTFAGIFSFYFPLGLTYLSGALKQAGYECRI
                     LDMDAADAKSGSFDFAHEYERYGKYVEALNNPEHPTWQHMRELIRAENPDFIGITAMT
                     TKFGSVIQTARQCKRLLPNVPVIVGGAHASTMPDLTLEIPEVDVVVRGEAEETLPDLL
                     NRLRSGDDLKSVLGISYRDGNVVCHNPDRPYVQNLDSIPLPDRGALMHPQNYSSEDMG
                     VMLNSRGCPFRCSYCFHMWQRRVRYRSVENILSEIRAVRAAYGTTQFHFKDDSFTVNR
                     KQVEALCAAIRREPYHINWSCTTRVDLIDDNLLRIMKEAGCNQVSVGIESGSKRILQE
                     TDKDITHEQILEAAKKLNQHRLFWSGYFMIGLPTETEADIRQTFAFLKRAKPYYAGLG
                     VYNPFPRTKLFDQGVQMGLLNPKPDLQHFLTTNPKDLFFIDPRRRV"
     gene            complement(1318..2268)
                     /locus_tag="PKO06_10820"
     CDS             complement(1318..2268)
                     /locus_tag="PKO06_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV70181.1"
                     /translation="MRILVTGGHGTVGSGLIKELRQRGHHVVSVDLLHANDEIGFSLR
                     TDVEKPLYARADVGCFREIERVFEVMGPFDYVFHAAAEFGRWNGEDYYERLWHTNAVG
                     TKNIIRLQERFKFRLIHFSSSEVYGDWPELMIETVMDQHEIKQMNDYAMTKWVNEMQI
                     RNSAIQYDTESVVVRLFNTYGPGEYYSPYRSVNVRFLYCAMMGHPWVVYRGHYRTSTY
                     LADTVRTLANITENFKPGETYNIGGNEYHSIEQLSDAILKVTGADPKLVTYKDSEILT
                     TKIKKVDISKSVRDLKHQNSYPLEEGIRLTYAWLRKAYDK"
     gene            complement(2293..>3209)
                     /locus_tag="PKO06_10825"
     CDS             complement(2293..>3209)
                     /locus_tag="PKO06_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNV70182.1"
                     /translation="KYVEDATRAILPVLREGNLVILESTSPARTCSHLIAPILAESGL
                     KVGEQIFLAHCPERVLPGQIMKEVVENDRVIGGINRESALAAQELYASIVKGQIFLTD
                     ATTAEMVKLVENSFRDVNIAYANELSMICDELGLNVWELISLANRHPRVSILRPGAGV
                     GGHCIAVDPWFIVDTCPQRARLIRTAREVNDSKPHWVVEKVKQAAERFKKPVIGLLGL
                     AFKQDIDDLRESPALDIARHLKKEKLGELLVSEPFVKSHPDFSLMKPEDVVKKSDIVV
                     LLVPHRAFKEIDKELLKPKVVIDTCGIW"
BASE COUNT          687 a          876 c          921 g          725 t
ORIGIN      
        1 cactcgtcgt cgtggatcaa tgaaaaacag gtctttcgga ttggtggtca ggaagtgctg
       61 caggtcgggc ttcgggttga gcagccccat ctgcacgccc tggtcgaaca gcttggtgcg
      121 tggaaacggg ttgtagacgc cgagcccggc atagtagggc ttggcgcgct tcagaaacgc
      181 gaatgtctgc cgaatgtcgg cttcggtttc cgtcggcaga ccaatcatga agtagccgct
      241 ccagaagagg cggtgctggt tcagcttctt ggccgcctcg agaatctgtt cgtgcgtaat
      301 gtccttgtcg gtttcctgca aaatgcgctt cgaaccactt tcaatgccaa ccgatacctg
      361 attgcagccg gcctctttca tgatgcgcag caggttgtcg tcgatcagat caacccgcgt
      421 cgtgcagctc cagttgatgt ggtagggctc gcgtcgaatt gcggcacaca gcgcttcgac
      481 ctgcttgcgg ttcaccgtga aggaatcatc tttgaagtga aactgcgtgg taccgtaagc
      541 cgctcgcaca gcacgaatct cggagaggat gttctcgacc gagcgatagc gcacccgcct
      601 ctgccacatg tgaaaacagt acgagcagcg gaaggggcac ccacgcgagt tgagcatgac
      661 tcccatgtct tctgacgaat aattctgcgg gtgcatgaga gccccgcgat cgggcagcgg
      721 aatcgagtcg agattctgca catacggtcg atcgggatta tgacagacaa catttccgtc
      781 ccggtagctg atgccgagaa cgctcttgag atcgtcacct gaacgaagac gattcaacag
      841 gtcgggcagg gtctcttcgg cctcgccgcg aaccacgaca tcgacttccg gtatttcgag
      901 agtcaggtca ggcatggttg aagcatgagc tccgccgacg ataaccggta cgttcggcaa
      961 aaggcgtttg cattgtcgcg ccgtctgaat gaccgaaccg aacttggtgg tcatggcggt
     1021 aatgcctatg aagtccgggt tctcggcacg aatcagctcg cgcatatgct gccaggtcgg
     1081 gtgctcgggg ttgttgagcg cttcgacgta tttgccgtag cgctcatact cgtgagcaaa
     1141 gtcgaacgaa ccggatttgg catccgcggc atccatatcg agaatgcggc actcatatcc
     1201 ggcttgtttg agcgctccag acagataggt gagtccgagc ggaaaataga aactgaagat
     1261 gcccgcgaag gtgcgcatcg ggggctcgat cagcagaatc ttgcgggtct tcattcccta
     1321 cttgtcgtaa gctttgcgca gccacgcata ggtcagacga atcccttcct cgagcgggta
     1381 actgttttga tgcttgagat cacgaaccga cttcgagata tcgaccttct tgatcttggt
     1441 ggtcagaatc tcggaatcct tatacgttac cagcttcggg tcagcgccgg tgaccttgag
     1501 aattgcgtca gaaagctgtt cgatcgagtg gtattcattc ccgccaatgt tataggtctc
     1561 acccggttta aagttttcgg tgatgttcgc cagcgtgcgc acggtgtccg cgagatacgt
     1621 cgaggtgcga tagtgcccgc gatagacaac ccaggggtgc cccatcatgg cacaatacag
     1681 gaatcgcaca ttcacggacc ggtacggact gtaatactcg ccgggaccgt aagtgttgaa
     1741 gagtcgcacc acaacagatt ccgtatcata ttgaatcgcc gagttgcgaa tctgcatctc
     1801 gttgacccac ttggtcatgg cgtagtcatt catctgcttg atctcatgct ggtccatgac
     1861 cgtttcgatc atgagttcag gccaatcgcc gtaaacctcg gaagacgaga agtgaatcag
     1921 gcggaacttg aagcgctcct gcaggcgaat gatgttcttg gtgccaacgg cattggtgtg
     1981 ccagagtctc tcgtagtagt cctcgccatt ccagcgtccg aattcggccg cggcgtgaaa
     2041 gacatagtcg aaaggcccca tgacttcgaa gacgcgttcg atctcgcgaa aacagccgac
     2101 gtcagctcgc gcatacagcg gcttctcgac atcggtgcgc agcgaaaaac cgatctcatc
     2161 attggcatga agcagatcga ccgagaccac gtgatggccg cgctggcgca gttccttgat
     2221 caagcctgag ccgacggtgc cgtgaccgcc ggtaaccaga atacgcatcg ttgttctcct
     2281 tgtggccgag acttaccaga taccgcaggt gtcgatgacg accttgggct tcagcagttc
     2341 cttgtcgatt tccttgaagg cgcggtgcgg taccagtagt accacgatat cggacttctt
     2401 gacgacatct tccggcttca tcaatgaaaa gtccgggtgc gatttcacaa aaggctcgct
     2461 taccagtagt tcgccgagct tctctttctt gaggtgacga gcgatatcga gcgcgggtga
     2521 ttcgcgcaag tcgtcaatat cctgcttgaa cgccagaccc agcaatccga taaccggctt
     2581 cttgaaacgc tcggcggctt gcttcacctt ctcgacgacc cagtggggtt tgctgtcatt
     2641 gacttcacgt gccgtgcgaa tgagtctcgc gcgttgtggg caggtgtcga cgatgaacca
     2701 ggggtcgacc gcgatgcagt gaccgccgac gccagcgccc gggcgcaaaa tactcacgcg
     2761 cggatgtcga ttggccaggc tgatgagttc ccagacgttg agcccgagct cgtcacagat
     2821 catcgatagt tcattggcgt aggcgatatt cacgtcacga aaactgtttt cgaccagctt
     2881 caccatttcg gccgtggtcg catcggtcag aaaaatctgc cctttcacga ttgacgcgta
     2941 gagctcctga gcggccagtg cggactcgcg attgatgccg ccgataacgc gatcgttttc
     3001 cacgacctct ttcatgatct gcccgggaag gacgcgttcg gggcagtgcg ccaggaagat
     3061 ctgttcgcca accttgagcc ctgattccgc cagaatcggc gcaatcaggt gggaacaggt
     3121 gcgcgcgggt gaggtcgact cgagaatgac caggttgcct tcgcgcagaa ctggcagaat
     3181 ggcccgagtg gcgtcttcga catacttca
//
LOCUS       DAORHD010000482         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34161_length_3209_cov_3.606214, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000482 DAORHD010000000
VERSION     DAORHD010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34161_length_3209_cov_3.606214"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="PKO06_10830"
     CDS             complement(<1..1212)
                     /locus_tag="PKO06_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV70183.1"
                     /translation="MNIARFSIRHPVTIIMGIFVLLTIGLMSVWELPTDMFPEITFPV
                     LSVVTRYSAAAPEEVENLVTKPLEEALATINGVKNITSTSQHGRSMINLEFDWEYDLD
                     AAANDVRDSIQYAKRFLPEDCELPVVVKIDLSKIPIWVGTMRGSRSFPALQRLAELTI
                     QPRLSRIEGVAAIGILSSEEEEVEISADLRALERHRIGFNLLYKLLSSENRNIPAGDI
                     NVGGHVLSLRTMGETNEVSRLGDLPIVAGVDGSILTLSRLASIRRIPRRDDVIIERNG
                     HPVIALQVMKQSGKNTVQVLDKVNAEIEKIRKSLPPDVVFENVFDQSEFINQSISAVR
                     VNLITGGLLAIAILFFFLRQISMVLIIGVAIPLSVLISFIPLYFLKYSLNTMSLGGLA
                     LGVGMLVDNAIV"
     gene            complement(1209..2516)
                     /locus_tag="PKO06_10835"
     CDS             complement(1209..2516)
                     /locus_tag="PKO06_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV70184.1"
                     /translation="MTRIFSDIQLSLWHSRVSALRISFLITFATLVILCRLQPAAAQE
                     GLLVEVTPARSAEISPLLTFPGTVISPHTARVSAEIGGHLEFLAGEPGVPLASGDVLF
                     RLNAENHRLSLDAALAARDIARRQLEFARAGTRAEEISRLQALEQHAAAALSQIRNEK
                     KRLAGLKAGQAISNAQWDDIEFRERMAIHQLEASQQSVQAARRGQTPEQIAIAAAQLD
                     QAQVQVRQAEKALRDTVVRAPFSSVLVQRYREPGENLAPGEPVVRIHRLDPLRVRFFV
                     GESQLSALRSARRLELHIPALSATCSARLVSLTPDCDEVTRRFPCEAEFANPQSLVWP
                     GLSAEIRVQLPSAQGTLIPLSALVDTQSGTGVFLVEQGLARFRPVRELSRTASQLLVE
                     GIAADAIVVSRGALGLRDGAAVQVRSAEIPPATSPDSASPTRR"
     gene            3017..>3209
                     /locus_tag="PKO06_10840"
     CDS             3017..>3209
                     /locus_tag="PKO06_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70185.1"
                     /translation="MKTSHWIALCCGLFTSCAVGAQNLDEVAAQPYQDRPVEGILSAP
                     SLAGEAARGELSTAASETSG"
BASE COUNT          709 a          868 c          997 g          635 t
ORIGIN      
        1 gacgatggcg ttgtcaacca gcatgccgac acccagagcg aggccgccca gcgacatggt
       61 gttgagagaa tacttcagaa agtagagcgg aatgaaggaa atcaacaccg acaggggaat
      121 agcgacacca atgatcagaa ccatactgat ttgccggaga aagaagaaca gaatggcgat
      181 cgccagcaga cccccggtga tcagattcac acgcacggcg ctgatcgact ggttgatgaa
      241 ttccgactga tcgaacacgt tctcgaacac gacatccgga ggaagactct ttctgatctt
      301 ttctatttca gcatttacct tgtcaagaac ttgtacagta ttcttgccgc tctgtttcat
      361 cacctgtaga gcgataaccg ggtgcccatt gcgttcgata atgacgtcgt cgcggcgtgg
      421 aatgcggcga atgctggcca gacgggagag cgtcaggatg ctgccatcca caccggcgac
      481 gattggcagg tcgccgagac gggacacctc gttggtttcg cccatggtgc gcagagaaag
      541 gacatgaccg ccaacattga tgtctccggc tggaatgttg cggttttcgg atgatagtaa
      601 cttgtacagg aggttgaaac cgatccggtg gcgttcgagc gcgcgcagat cagcgctgat
      661 ctcgacctct tcttcctccg aagagagaat gccgatggcg gccacgcctt caatgcgact
      721 gagacgcggc tgaatcgtca attccgccag acgctgtaaa gccggaaagc tgcgggaacc
      781 acgcatggtg ccgacccaga tcggtatctt ggagaggtcg atcttgacca ccactggcag
      841 ttcgcagtct tcgggaagaa accgcttggc atactggata ctgtcacgga cgtcgtttgc
      901 ggcagcatcg agatcatact cccagtcgaa ctccagattg atcatcgatc gcccatgctg
      961 tgacgttgag gtaatgttct tgacgccgtt gatggtggcc agggcttctt cgagcggctt
     1021 ggtaaccagg ttttccactt cctccggagc ggcggcggaa tagcgtgtca ccaccgagag
     1081 taccggaaag gtgatctcag gaaacatgtc ggtggggagt tcccataccg acatcagccc
     1141 gatggtcagc aacacgaaga ttcccatgat gatggttacg ggatgccgaa tggaaaaccg
     1201 ggcgatgttc atcgccttgt gggagacgca gaatcagggc tggtggcagg tgggatctca
     1261 gctgagcgaa cctgcaccgc agctccatca cgtagcccga gggctccacg gctgacgacg
     1321 attgcgtctg cggcgattcc ttcgaccagc agctgactcg ctgtacggga caattcgcgc
     1381 accggtcgaa accgggcgag accttgctcg acgagaaaaa caccggttcc ggattgggtg
     1441 tcaaccaatg ccgacaaggg aatcagagtg ccttgagcgg acggcagctg cactcgaatc
     1501 tcggcggaaa gcccgggcca caccagggac tgcgggttcg caaactccgc ctcgcaggga
     1561 aaacggcggg tgacctcgtc acaatcggga gtgagtgaga ccagacgggc tgaacacgtc
     1621 gcggataacg cgggaatatg gagttcgagc ctgcgggcgg aacgaagagc ggacaactgg
     1681 gattcgccga cgaaaaaacg cacccgtagt ggatcgagcc ggtgaatacg cactaccggc
     1741 tcacccggcg cgaggttttc accgggctca cgatatcgct gaacgagtac cgatgaaaac
     1801 ggagctctta caacggtgtc gcgaagcgct ttctctgctt gacgaacctg gacttgcgcc
     1861 tggtcgagtt gggccgccgc gatggcgatc tgctccggag tctgtccgcg acgagcggcc
     1921 tgcaccgact gctgtgatgc ttcgagctga tgtatcgcca tccgttctcg aaactcgatg
     1981 tcatcccatt gggcattcga gatcgcctgc ccggccttga gtcccgcaag acgcttcttc
     2041 tcgtttcgga tctgggagag agccgcggcg gcgtgttgtt cgagtgcctg aaggcgactg
     2101 atttcttccg cgcgcgtgcc ggcacgggca aactcaagtt gccgccgcgc gatgtcgcga
     2161 gccgccaaag ctgcgtccag actgagccga tgattctcgg cgttcaaccg gaacagcacg
     2221 tcacccgagg caagcgggac cccgggttcc cccgcgagaa actcgagatg gccaccgatt
     2281 tccgcggaaa cacgtgcggt gtgtggcgag atcacggtgc ctggaaacgt caggagcgga
     2341 gaaatctccg cggagcgcgc cggtgtcacc tcgacaagca gaccttcctg ggctgccgcg
     2401 ggctgcaggc ggcagagaat gacgagtgtg gcgaaggtaa tcagaaaaga gatgcgaagc
     2461 gccgatactc gggaatgcca gagggaaagc tgaatgtcac tgaaaatgcg tgtcatcaat
     2521 cctctggggt gtctcgatgt ggttgagcgg ggagcggggt gggcaaggga tctgcgggtt
     2581 cccgggtttc cggaatcggt gacgatgggg cctgcggtga agaaggtttg cgaccacatt
     2641 tgaccggaat ctctcgcgag ggggattcgg ccggagccga agttccctgg gaggggagcg
     2701 ggcatcgctt gcgtgcgaga cactccgcgc atcgcgcatc actgcaccac tggcagaacc
     2761 cacagtcggt acagggatgc ttcttgtgtt tacactcctg aaacggattg tagacgttgt
     2821 atcgatcgta aatacttttc ttgtcgtcac tcatggtgcg ggttcccgga aaaaccggac
     2881 agacagagta taggcgctgt ccttccccat gtcaaagatc gccgccgggc cttctcctgt
     2941 tccatagagc gtgaaaactt ctggcgtgtg tcatggtaat ataaagatga aatctctgtg
     3001 ggctgggaga cgatcgatga aaacaagtca ctggatagct ttgtgttgcg gtctgttcac
     3061 gtcctgtgcc gtgggagccc agaacctcga tgaggtcgcg gcacaaccct atcaggatcg
     3121 acctgtcgag ggcatactgt cggccccctc tttggctggc gaagccgctc gcggagagtt
     3181 gagtacagct gcgagcgaaa cttccggcg
//
LOCUS       DAORHD010000483         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34225_length_3204_cov_3.018101, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000483 DAORHD010000000
VERSION     DAORHD010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34225_length_3204_cov_3.018101"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1188
                     /locus_tag="PKO06_10845"
     CDS             73..1188
                     /locus_tag="PKO06_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70186.1"
                     /translation="MAGGIAHDFNNLLMAILGHSDLARLELPEKSPAQQHLEQIQTAS
                     ERAADLCRQMLAYSGRGTFLIEPIDINQLIDELMNMLHTAVSKKAEICLHQSRDLPNV
                     HADATQIRQVVMNLVLNASDALGEQGGTITVTTERREVSAEDLVNSLTRETLEPGPYV
                     CVEIADTGCGMSEEVLQRIFEPFFTTKFTGRGLGLSAVLGIVKGHKGFLRVKSRPGYG
                     TCFTIGLPAHESRRVAEPAAADTANVKPTGLILFVEDEPLLQQLGTKMLTSLGLKSIV
                     ASDGNEAVRLYQQRKSEISVIMLDMTMPYMNGEEAYRELRKIDPNVVVILASGFTESE
                     INERFDGCPLAGFLQKPYTLASLKAALGKALRQTEQK"
     gene            complement(1270..3066)
                     /locus_tag="PKO06_10850"
     CDS             complement(1270..3066)
                     /locus_tag="PKO06_10850"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNV70187.1"
                     /translation="MNEKLIERFKTTFEKLKPIAGQMLVELGDESRVLERARHKPRVL
                     VVGEFNTGKSSLVNSAIGETLLPVGVTPTTSLTTVLQHGPFRLEIKPIGQKDPIKVEP
                     GKDQSFGYGIKEGGFDWAGLKQLLTDQKNIDQIEQVLITHPAAPESIVIIDTPGINDL
                     SKSRAEVVYGFIPQADIVVFLINATKPFSESERVFLEERILAGDLKKVLFVVNHLDEI
                     EEHERAELIQDIHDQVRDAVNKGLDNLNQILGSTLHPKIEKVTLLPACAKLMAPLDGK
                     STRGSIGFATGGNRSENELADMNRAVWQQILKAATADREKEVEATLHHHLRRSAMKIT
                     RAIDATLQARQSGNDAMLQRLRDNSDRLKKLRDTFRSSEVKIKKAEAELKERIHAQIE
                     KTVAEMANTGIWNRDPNMINQRLKDLYEYITNSMKSRLDELYAELGQDFDAILEDKRF
                     MEEHHLQIQYDLSDLPNKIVSSLSFAYLAAIFFGISVGMFAGAAYFASQVIANKRSIK
                     QYLLSMRVKEDKLLEVKKELLYAVDQEIEYAIDFVRQSLSQRADIIQGDVRNLVFALT
                     RDTKLDVAAIQKSLESVQPEINAFLLPEKPVT"
BASE COUNT          665 a          909 c          902 g          728 t
ORIGIN      
        1 aatcatgcgc aatcctgccg gccatgattc cgaaaccttc tccagacgca gaaactcgaa
       61 agtctcggaa tcatggccgg cgggattgcg catgatttca ataacctgct catggctatc
      121 cttggacact cggacctcgc acgtctcgaa ctgcccgaga agtcgccggc gcagcaacac
      181 ctcgagcaga ttcagaccgc gtcagaacga gccgcggatc tgtgtcgtca gatgctggcc
      241 tactcgggtc gaggcacgtt cctgatcgag ccgattgaca tcaatcagtt gattgacgaa
      301 ctgatgaaca tgctgcatac cgccgtttcg aagaaggccg aaatctgtct gcatcagtcc
      361 cgggatctgc caaacgtcca tgccgatgcg acgcagattc ggcaagtggt gatgaatctt
      421 gtcttgaatg cctcggacgc cctgggggaa cagggcggaa ccatcacggt caccacggaa
      481 cgtcgcgaag tgagcgctga agacctggtg aattccctga cccgcgagac actcgaaccc
      541 gggccatatg tctgtgtcga gattgccgat acggggtgcg gcatgtccga agaagtgctc
      601 cagcgcatct ttgaaccctt cttcacgacc aagttcaccg gcaggggctt gggactgagc
      661 gcggtactcg gcatcgtcaa agggcacaag ggatttctcc gcgtcaagtc gcgacccgga
      721 tacggcacgt gcttcaccat cggtttgccc gctcacgaat cgcgtcgcgt cgctgagccc
      781 gcggccgccg atacggcgaa tgtgaagcca accgggttga tcttgtttgt cgaagacgag
      841 ccacttttgc agcaactggg aacaaagatg ctcacctccc tcggactgaa atcgattgtt
      901 gcgtctgacg gaaatgaagc cgttcgtctg taccagcaga gaaagagcga gatatcagtg
      961 atcatgcttg atatgaccat gccgtatatg aatggcgagg aagcctaccg cgaactgcgc
     1021 aagatcgatc cgaacgtcgt cgtcattctg gccagcgggt tcaccgaatc ggaaatcaac
     1081 gagcgctttg atgggtgtcc actggcaggt ttcctgcaga aaccctatac gcttgcctcc
     1141 ctgaaggccg ctctcgggaa agcattgcgc cagacggagc agaaatgaaa acaccggggt
     1201 gccgaagggc acacccggtg ttgatcaaga acgatcagag ttcgcctgag ctcgcgacgg
     1261 agtgcaagat cacgtcaccg gcttctcggg caacaggaag gcgttgatct cgggttgaac
     1321 gctttcgaga ctcttttgaa tcgcggcgac atcgagcttc gtatcgcggg tcagcgcgaa
     1381 caccagattt cgaacatcgc cctgaatgat atcggcacgc tgactcaatg actgacgaac
     1441 gaagtcgatc gcatactcga tctcctgatc gacggcatac aggagttctt tcttcacttc
     1501 gagcagcttg tcctctttca cgcgcatcga gagcagatac tgcttgatcg agcgtttgtt
     1561 ggcgatcacc tgtgacgcga aatacgcggc cccggcgaac atgccgacgc tgatgccgaa
     1621 gaagattgcc gcgaggtaag cgaagctcag agacgagaca atcttgtttg gcaggtcgga
     1681 caaatcatac tgtatctgca ggtgatgctc ttccatgaac cgcttgtctt cgagaatcgc
     1741 gtcgaagtcc tgtccgagtt cggcatacag ctcatcgagc cgcgacttca tcgagttggt
     1801 gatgtactca tacagatctt tcagccgctg gttgatcatg ttcggatcgc ggttccagat
     1861 gccggtattc gccatttcgg cgacggtttt ttcgatctga gcgtggatgc gctctttcag
     1921 ctcagcctcg gccttcttga tcttgacctc actcgagcgg aaggtgtcgc gcagcttttt
     1981 cagtctgtcc gagttgtcgc gcagtctctg cagcatggca tcgttgcctg actgccgtgc
     2041 ctgcagtgtc gcgtcgatcg cacgggtgat cttcatggcg ctgcggcgca gatgatggtg
     2101 cagcgtggct tcgacttcct tttcgcggtc ggcggtcgct gccttcagaa tctgctgcca
     2161 gacggcgcgg ttcatgtcag ccagttcgtt ttcgctgcgg ttaccgcccg tcgcgaaccc
     2221 gatcgaccca cgggttgatt tcccgtcgag cggagccatc agcttggcac atgccggcag
     2281 aagggtgacc ttctcgatct tcgggtgcag agtcgagccg agaatctggt tcagattgtc
     2341 caggcccttg ttcacggcat cgcgcacctg atcatgaata tcctgaatca gttcggcgcg
     2401 ctcgtgctct tcgatctcat caaggtgatt caccacgaaa agaaccttct tcagatcgcc
     2461 cgcgagaatg cgctcttcca ggaaaacgcg ctcggactcc gagaatggct tggtggcatt
     2521 gatcagaaag accacgatat ctgcctgcgg aatgaacccg tagacgactt cggctcgtga
     2581 cttcgacaga tcgttgatac ccggcgtatc gatgatgacg atcgattcgg gagcggcggg
     2641 gtgggtgatc agaacctgtt cgatctggtc gatgttcttc tgatcagtca acaactgttt
     2701 caacccggcc caatcgaaac cgccttcctt gatgccgtag ccgaacgact ggtctttgcc
     2761 cggctcgacc ttgatcgggt ctttctgccc gatcggcttg atctcgagcc gaaacgggcc
     2821 gtgctgcagc acggtggtca gtgatgtggt cggcgtgacg cccaccggca gcaacgtttc
     2881 gccgatcgcc gagttgacga gcgaggactt gccggtattg aactcaccga cgacgagcac
     2941 gcgtggcttg tggcgcgccc gctcgagcac acgactttca tcgccgagtt ccacgagcat
     3001 ctgcccggca atgggtttca gtttttcgaa cgtggtcttg aagcgctcaa tcagtttttc
     3061 gttcatatac ttttctcaac acgtatttct ggtcttcggc atcgatcttg acctggtcgt
     3121 tcgaggcaat gacccgattg atcaatgcct cggcgatcat ctcttcgaac cgctgaatct
     3181 cgcgccgcat ggggcgagcg ccaa
//
LOCUS       DAORHD010000484         3201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34264_length_3201_cov_4.161793, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000484 DAORHD010000000
VERSION     DAORHD010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34264_length_3201_cov_4.161793"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /locus_tag="PKO06_10855"
     CDS             <1..2334
                     /locus_tag="PKO06_10855"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70188.1"
                     /translation="RRWAENLLSSQMGKLVSLEVLRRTVGRGREVLYQGKRAFLQCLP
                     VTFSDQKGWAMIVLALWNLPENWELRHALRSLSKTGQSVGGFRWSQQKGLSTNPLSQN
                     LLAGLSSHLLSGTPFWHWRDTVFYSRLWDQNPDLILTTAVRPDSRRLSSQQITFTTLR
                     LILWMAAALLVFYHLAPVTAHTLSSSLSQQFQTISLLLILPLLVALALCQFHYASQIR
                     WLTRQQMTRQISASLQQLEQELLDSYSVFGDELERTVRLLSQEKRDATEVMQRMQQRF
                     ASFGLESFFFLPHQGEFVESGWMKKSSDLAGIQFQIITESVERLGMQIDRSRFPASLK
                     RAKSEYAFLVDQVVSSSSYGKLLRLKFTRATMAVFHTVHRNASGIPVAYFYASFRDPE
                     LRNAFLARLRKTRRTSSRPSILVRRNEIPHVRKRALPVEYTDSLDQAEATGRVIQTSF
                     TRGGERFSLQARSLPNHSLTVLACVNLDLSNPFLWISQRFLLLLFAGCLLAWWLGSRL
                     MRHYLLDPVLELRGQVLALERGSLDCCWNHVHDDQLGRLCQGFNALVKELQQKSRMSV
                     FLRRDLLESPQRTTMSRTELVTVAFFGLRNFTACERQLAPDQAFALMNGFLSLCEETT
                     RRHGGVIDKFIGDTAMAVFRQQGDFQSWDSALTTARDIVQQFQAWRDQRVETASLACG
                     AGIAFGEIIAGCVGSRNRRLDFTVIGDLVNVAARLEKLAGRADTPVILTHGLPPETAL
                     VGLRVCPLPARTVKGREEQVSMIGFTLADDLIKRRLT"
     gene            2331..>3201
                     /locus_tag="PKO06_10860"
     CDS             2331..>3201
                     /locus_tag="PKO06_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70189.1"
                     /translation="MRHSPHSRSAAMTSGIALFLLLLAIGLTLGHHSASEEQTFRRQR
                     LDQCHNLLPTVREYLQPESLLQHRVLSLLRTTDRFDEVQAARLADDLQKLIGPPLKLV
                     LVRDDQTLLPICGFSAPQLEQLQVLLRFLRESHAGIKPVDPQQRFNEALRLLFGRSIR
                     PSQPLWNLTHAQATSLEGQPGTVFFHTGFTHVRLSHYFRRYLSPSRSNLPTSAFTGQL
                     IAFVPHTVLSDDWLREQIPYPLHQLHIGNSLFFDWDDQTETLKSRTPASASTPFPDFE
                     RTISALETLLKQRV"
BASE COUNT          643 a          982 c          828 g          748 t
ORIGIN      
        1 cgacgctggg ccgagaatct gttgtcctca cagatgggaa aactggtttc tctcgaagtt
       61 ctgcgccgta cggtgggacg agggcgcgaa gttctgtatc aaggcaaacg cgcctttctc
      121 caatgcctgc ccgtcacgtt cagtgaccag aagggttggg ccatgatcgt tctcgctctc
      181 tggaacctgc ccgaaaactg ggaactcagg catgccctcc ggagtctctc gaaaaccgga
      241 cagtcagtcg gcggctttcg ctggtcacag cagaaaggtc tcagtaccaa tccgctcagc
      301 cagaatctac tggcgggctt gtcatcacac ctgctctcag gtactccgtt ctggcactgg
      361 cgcgatacag tattttattc gcgactgtgg gaccagaatc ctgatctgat tctgaccaca
      421 gccgtgcgcc ctgacagccg ccgactctcg tcccagcaga tcacattcac cacgctgcgc
      481 ctcattctct ggatggcagc cgcactgctg gtgttttacc atctcgctcc cgtgaccgcg
      541 cacacgctgt cgagcagcct gtcgcagcag tttcagacca tctctttgct cttgattctt
      601 cctctgttgg tcgcgctcgc cctgtgtcag tttcattacg cgtctcagat tcgctggctg
      661 acccggcaac agatgacccg acaaatctcg gcttcattgc agcaactgga gcaggaactc
      721 ctcgactcct actccgtgtt cggtgacgaa ctcgaacgga ccgtcaggct gctgtcccag
      781 gaaaaacgcg acgcgactga agtcatgcaa aggatgcagc aacgatttgc ctcattcggt
      841 ctcgagagtt tcttttttct gccacatcag ggcgaattcg tcgagtccgg atggatgaag
      901 aaaagcagcg acctcgccgg gatccagttt cagattatta ccgaatcagt cgaacgactg
      961 ggcatgcaga tcgaccgctc tcgctttccc gcaagtctga aacgtgcgaa gagtgagtat
     1021 gcctttctgg tcgaccaggt cgtcagttca tcctcttacg gaaaactgct tcggctcaag
     1081 ttcacgcggg cgaccatggc ggtttttcac acagtccatc gaaacgccag cgggatcccc
     1141 gtcgcgtatt tttacgcttc gtttcgcgat ccagagctcc gcaatgcctt cctcgcacgg
     1201 ttgcggaaaa cccggcgtac atcttcccga ccctcgattc tggtacgtcg aaacgagatt
     1261 ccgcacgtgc gcaagcgcgc tcttcccgtt gaatacaccg actctctcga tcaggcagaa
     1321 gccacgggaa gagtgattca aacgtcattt acccgcggag gcgagcggtt ctccctgcag
     1381 gcgcgctctc tgcccaatca ctcgctcacc gttctggcct gcgttaatct cgatctcagt
     1441 aaccccttcc tgtggatatc gcagcggttt ctgttacttc tgttcgctgg ttgtctgctg
     1501 gcctggtggc tcggtagtcg tctcatgcga cactaccttc tcgatcctgt gctcgaactg
     1561 cgcggtcaag tgctcgctct cgagcgcggc agcctggact gttgctggaa tcatgttcac
     1621 gatgatcagc tgggccgtct ctgccagggt ttcaatgccc ttgtgaagga actgcagcag
     1681 aagagccgca tgtcggtgtt tctcagacgc gatctgctgg aaagtccgca gcgaaccacg
     1741 atgtcacgca ctgagctggt gacggttgct ttcttcgggt tgagaaactt caccgcctgt
     1801 gagcggcaac ttgctccgga tcaagcattt gccttgatga atgggtttct cagtctctgt
     1861 gaggaaacca ctcgccgaca tggaggtgtc atcgacaaat tcatcggtga cacagcgatg
     1921 gctgtctttc gccagcaagg ggactttcag agttgggaca gcgctctgac aaccgcgcgc
     1981 gatatcgttc agcagtttca ggcttggcgc gatcagcgcg tcgagacggc atcactggcc
     2041 tgtggagccg gaatcgcctt cggcgagatc attgccgggt gcgtgggatc acgcaatcga
     2101 cgactcgatt tcaccgtcat cggtgatctg gtgaatgtgg ccgcccgact cgagaagctg
     2161 gcgggtcgcg cggatactcc cgtgatattg acccatggtc tgcccccaga aaccgctctc
     2221 gtcgggcttc gcgtttgtcc gctgccggcc cgcacggtga agggacggga ggaacaagtc
     2281 tcgatgatcg ggtttactct cgcggacgac ctgataaagc ggaggctcac atgaggcata
     2341 gcccccactc acgttcggcc gccatgacct caggtatcgc ccttttcctc ctgctactgg
     2401 ccatcggctt gaccctgggt caccactctg cctcggaaga gcagactttt cgcagacagc
     2461 gcctggatca gtgtcacaat cttctgccaa cggtacgaga gtatctgcaa ccggagagtc
     2521 tgctgcagca tcgtgtcttg agtctgctgc gaacgaccga tcgcttcgac gaagtacagg
     2581 ctgcgcgtct cgcggacgac ctgcaaaagc tgatcggacc gcctttgaaa ctcgtgctgg
     2641 tgcgagacga tcagacactc cttcccatct gcggcttttc ggccccccaa ctcgagcaac
     2701 tgcaagttct cctccgcttt ctgcgcgagt cccatgccgg catcaagccg gtcgacccgc
     2761 aacagcgatt caacgaggcc cttcgtctgc tctttggccg atccatccgt ccttcacagc
     2821 cactctggaa tctgactcac gcacaagcaa catcattgga gggacaaccc ggaacggttt
     2881 tctttcacac cggatttact catgtccgtc tcagccacta cttcagacgc tatctctctc
     2941 cctctcgatc gaacttgcca acatcggcat ttaccggaca actgatcgcc ttcgttccac
     3001 acaccgtgtt gagtgacgac tggttgcgtg aacaaatacc ttatccactt caccagcttc
     3061 acataggcaa tagtctcttt ttcgactggg acgatcaaac agaaaccctg aaaagccgaa
     3121 caccagccag tgcatcaacg ccattcccgg attttgagcg taccatctcc gcacttgaaa
     3181 ctcttctgaa acaacgggtc t
//
LOCUS       DAORHD010000485         3198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34318_length_3198_cov_3.979955, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000485 DAORHD010000000
VERSION     DAORHD010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34318_length_3198_cov_3.979955"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..2523
                     /locus_tag="PKO06_10865"
     CDS             148..2523
                     /locus_tag="PKO06_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70190.1"
                     /translation="MSSGSGEQELIAQLQSDKRSVRVAAIVQLAKVSRDRQVLLKLRD
                     LAQSGDREVSFFAAQAVARIQSRLGEQSADQASGAGLTREKLIKPLKDEIAPLLALIR
                     QNPAVIAEELRLAVADFLSRYGEAADGQIVKTWLESCQDNLGLYYIDALESLNPSLLE
                     PLLPALLASALPLVRSRAIVALQKIDPEEALAHLQELLASRQPEQRLAGFRNAFFFPF
                     EQIREVVLAILPEETDREVLQAASILIASNPEPETALRLLDMIDTVGKEQGKRLTEIF
                     HELCGALKVSGLVAPELATPEKFLERWKKERLQKFLRDLEVQLPVATPARREAIESWL
                     KKNLSHPAVRDFIRRLAQNQATEDLARKLLPAGALQGGAPASGSSSAEASAIPPTARQ
                     APRQPLTDAEKISRLAALEEPVAVDVLAWVRAEAGQGAPAVRAEALLALSRLSPKPED
                     AALGEAGIINADSQVVSASVKLLETIDPARLVPFLSIIWSSEDPRVRVRGLKVALQHD
                     QKTAVEEFRRMVRSADPAQRAEAVAGVFHFPFALVESILLDALRHETHGAIARQILVV
                     LLMNPKRQMLTALDAMQGKVAGSVAILIAQARIDLYDLLLKLQLIAPEGTTAETEKET
                     PATSAKPYAVSRVREEIRKREAAAWRSPALPDADKARGAGGGWNTTALVAVAIVLLAI
                     LPLLFLRRPPPPPRFDPQADDQRFSTDSSRKIPLEFRMNRLCRLRGTVTTRHPDGSIY
                     VETESGRLRVFLQRQASAPAQIASGPVEVEVIPYRKSKYGEIWAEGHALRQ"
     gene            complement(2459..>3198)
                     /locus_tag="PKO06_10870"
     CDS             complement(2459..>3198)
                     /locus_tag="PKO06_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70191.1"
                     /translation="VFADLHHDPAQADVTFENVQARYRTLFLFNKANQLNGLVIGTGD
                     LSETALGWCTYNGDHMSHYAVNVGIPKTLVRWLVDWVSQQPAFSACHQVLQDIVATPI
                     SPELLPMKKGAISQKTEDLIGPYELHDFFLYHFVRWGSGPRRIFSLACQAFERQYDAD
                     TILHWLRLFIKRFFQNQWKRSVMPDGPKIGSVALSPRGDWRMPSDVDMADWMRELDGV
                     ATGGTKTTVATRAPRPRSRRTSTSGRE"
BASE COUNT          610 a          931 c          998 g          659 t
ORIGIN      
        1 cgtcatcacg ctgggagatg accatcagct tgaaacaggt ttctgcggcc tcgcgccgtt
       61 acttgccgaa gcctttcgca tggtcggtct cacacgtttg gctcgggtgt ttcctacggt
      121 tgatgaagcg gtgcgggggt tactcgaatg agctctggca gcggcgaaca ggaactgatc
      181 gcccaactgc agtccgacaa gcgcagcgtg agggtggcgg ctatcgtgca gctggccaag
      241 gtttcgcgcg atcgtcaggt gttgctcaag cttcgagatc tcgcgcagtc aggagatcgt
      301 gaggtcagtt tctttgcggc gcaggcggtg gcccgcattc agtctcgcct cggcgaacag
      361 tcggccgacc aggcctctgg agcgggattg acgcgcgaga agctgatcaa gccgctcaaa
      421 gatgagattg ctcccctcct ggcgctgatt cgtcagaatc cggcggtgat tgccgaagaa
      481 ctgcgtctcg cggtggctga tttcctgtcg cgttacggag aagccgctga tggacagatt
      541 gtcaaaacct ggctcgagag ctgtcaggat aaccttggcc tgtactatat cgatgccctt
      601 gaatctctga atccaagctt gttggaacca cttctaccgg cattgctggc gtctgcgctg
      661 ccgctggtcc gctcacgggc gatcgttgcc ttgcagaaga ttgaccccga ggaagcgctg
      721 gctcacctgc aggaactgct ggcctcgcgt cagcccgagc agcgattggc gggtttccgc
      781 aatgcgttct ttttcccgtt cgaacagatt cgtgaagtcg tcctggcgat cttgcccgaa
      841 gaaaccgatc gggaggtcct gcaggccgct tccattctga ttgccagcaa ccctgaaccc
      901 gagaccgcgc tgcggttgct ggacatgatc gataccgtcg gcaaggaaca ggggaagcgt
      961 ctgaccgaga tttttcatga actctgcgga gctctgaagg tttccgggct ggttgcccct
     1021 gagctggcaa cccccgagaa gtttctcgaa cgatggaaga aagaacggtt gcagaagttt
     1081 ctgcgcgacc tcgaggtgca gcttcccgtc gctactccag cgcggcgtga agctatcgaa
     1141 tcgtggctga agaaaaatct ctcgcatccc gccgtcagag atttcattcg acgcctggcg
     1201 cagaatcagg ccaccgaaga tctcgcccga aaattgttgc cggcaggagc gcttcagggt
     1261 ggggctcctg catccggttc cagttctgcc gaggcgtccg cgattccgcc gaccgcgaga
     1321 caggcaccac gtcagccgtt gaccgatgcc gagaagattt cgcgcctggc agcgctcgaa
     1381 gagcccgtcg cggtcgacgt actcgcctgg gtgcgcgcgg aagcggggca aggggctcct
     1441 gccgtgcgtg cagaagcgtt gctggccttg agtcggcttt cgcccaaacc cgaagatgcc
     1501 gcgcttggcg aagcgggaat catcaacgct gactcccagg tggtcagtgc gtcggtcaag
     1561 ctgctcgaaa ctattgatcc ggcgcgtctg gtgccattcc tctccattat ctggtcgagc
     1621 gaagatccgc gtgttcgcgt tcgcgggtta aaagttgcgc tgcagcatga tcagaagacg
     1681 gcggtcgaag agtttcggcg catggtacgc tccgctgatc cggcgcagcg cgccgaagcc
     1741 gtggccggtg tgtttcactt tcccttcgcc ctcgtcgagt cgattctcct cgatgccctg
     1801 aggcacgaga ctcacggagc gattgcccgg cagattctcg tcgtgctgct gatgaatccg
     1861 aagcgtcaga tgctcacggc acttgatgcc atgcagggca aggtcgccgg gtcggtggca
     1921 attctgatcg ctcaggcgag gatcgatttg tacgatctgc tgctgaagct gcagttgatt
     1981 gctcctgaag gaacaacagc agaaacagaa aaagaaacgc ctgcgacctc cgccaagccc
     2041 tatgccgtca gccgggtgcg agaagaaatt cgaaaacgtg aggcggcggc gtggcggtct
     2101 cctgctctgc ctgatgccga taaagctcgc ggagccggcg gcggttggaa caccaccgct
     2161 ctggtggccg tggccattgt gcttctggcg atcttgccgc ttctctttct gcgtcggccc
     2221 ccgccaccac cgcggttcga tccgcaggcc gacgaccaac gcttttcaac cgactcgtcg
     2281 cggaagatcc ccctcgagtt tcgcatgaat cgcctgtgtc gactgcgcgg aaccgtgact
     2341 acgcgacacc cggatggctc gatctatgtc gaaacggaaa gcggccgtct gcgggtcttc
     2401 ctgcaacggc aggcttcagc gcccgcacag attgccagcg ggcctgtcga agtcgaggtc
     2461 attccctacc ggaagtcgaa gtacggcgag atctgggccg aggggcacgc gttgcgacag
     2521 tagtcttcgt gccgcccgtc gccacgccgt cgagttcgcg catccagtct gccatgtcga
     2581 cgtccgaagg catgcgccag tcacctcgcg gagaaagggc aaccgagccg atcttcgggc
     2641 catcgggcat gaccgatcgt ttccactgat tctggaagaa gcgcttgatg aagaggcgca
     2701 gccagtgcag aatcgtgtcc gcgtcgtatt gccgttcgaa cgcctgacaa gccagcgaga
     2761 aaatgcgtct ggggccgctt ccccagcgaa cgaaatggta caaaaagaaa tcatgcagtt
     2821 catacggccc gatcagatct tcggtttttt gcgagatggc tcccttcttc atggggagca
     2881 gttcggggga gatcggcgtg gcaacgatgt cctgcagcac ctggtggcag gcggagaacg
     2941 ccggttgctg actcacccag tccacgagcc agcgtacgag ggtcttggga atgcccacgt
     3001 tgacggcata gtgcgacatg tggtcgccgt tataggtgca ccagccgagg gccgtttctg
     3061 acaggtcccc ggtgccgatg acgaggccgt tgagctggtt ggctttgttg aagagaaaca
     3121 gagtgcgata gcgggcctgg acattctcga acgtcacatc ggcctgcgcc ggatcgtggt
     3181 gcaggtcggc aaaaacct
//
LOCUS       DAORHD010000486         3187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34476_length_3187_cov_4.540230, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000486 DAORHD010000000
VERSION     DAORHD010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34476_length_3187_cov_4.540230"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PKO06_10875"
     CDS             complement(<1..1386)
                     /locus_tag="PKO06_10875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV70192.1"
                     /translation="MARGPDADKSREELLAELAELRQQLTACRPESPVPAEPLALENS
                     PPFQNWQAVIDAVGSTIWVLDAACRIHMTNRSTHDVVPGANESPLGRRCWQVMHGTEE
                     PIATCPIRRARQTKQRESAVIQVGRRWLEVTVDPLLSPTREITGFVHLTTDVTARVQN
                     EELRRTSERRVRALLDAISESAFLMDVEGRVLETNDVMLKRLQIDRDQLAKTNVYDLL
                     PPAVAEQRRKYAQQVIETASPVHFEDERFGRTILNSIYPVTGSDGRVDALAIYGMDVT
                     SERQTRREIEANRKRTAELNSLLQLVLDTIPVRIFWKDRNSAYLGCNQLFARDAGRTS
                     PEEMIGKTDAEMGWHEQAALYVTDDQKVITSGTPKINFEECQTTPDGRRIWLRTTKVP
                     LRDETGTVFGVLGTYEDITERKQIETSLRESEKRYRSLFQCHHSVQLVIDPATGQIVD
                     ANPAACRFYGYD"
     gene            complement(1536..2678)
                     /gene="ruvB"
                     /locus_tag="PKO06_10880"
     CDS             complement(1536..2678)
                     /gene="ruvB"
                     /locus_tag="PKO06_10880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009592741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNV70193.1"
                     /translation="MKRQKPPLASDSDEELRSGERSEEPGVPLAAESAGAILSPHAHP
                     DEETEEVVAPALRPRRFQEFIGQRNIVDKLKIFIEAARQRSEPLDHTLLLGPPGLGKT
                     TLANVIAAEMGVPIKITSGPVLNRAGDVAALLTGLQAGSVLFIDEIHRLSKTVEEVLY
                     PAMEDYHFDVLIGKGPTARSIRMQVKPFTLVGATTREGDVAAPLRGRFGVQLRIDFYS
                     LEELTDLLQAVARRLSYPISGEAAGEIARRSRGTPRVAIRLFKRMRDVAQVDRRETIE
                     MDVVQRGMEMLKVDRLGLDYIDRRILDVLVNRFDGGPVSLDTLSVSVGEEADTIYDVY
                     ESFLIQIGFLSRTSRGRIATRLAYEYLGKRPPEPSRNDAQLRLFND"
     gene            complement(2693..>3187)
                     /locus_tag="PKO06_10885"
     CDS             complement(2693..>3187)
                     /locus_tag="PKO06_10885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNV70194.1"
                     /translation="EVGSGARLLCHLVWREDGPQLFGFSTEAERQIFRLLMRVNKVGP
                     KLAVNILSASTPAGLVGMILREDVTGLTALKGVGAKIASRLVVELKDELLKSGFLAED
                     EAAKPVARRGAVPHEKDLREALENLGYTGREIDRAIRDLANDLALDADLTAMLERVLQ
                     YFSK"
BASE COUNT          621 a          985 c          905 g          676 t
ORIGIN      
        1 atcgtaccca tagaatcgac acgccgccgg attcgcatcg acgatctgac cggttgcggg
       61 atcaatgacc aactgtacgg aatgatggca ctggaacaga ctgcgatagc gtttttcgct
      121 ttcgcgcaga ctcgtctcga tctgctttcg ctcggtgatg tcctcgtagg ttccgagaac
      181 gccgaacacc gttccagtct catcgcgcaa cggcaccttc gtcgtgcgta accagattcg
      241 acgcccatcc ggagtcgtct ggcactcctc gaagttgatt ttcggtgtgc ccgacgtgat
      301 gaccttctga tcatcggtga catagagagc ggcctgttca tgccacccca tctcggcatc
      361 ggttttccca atcatttctt cgggagacgt tcggccggca tcccgggcaa agagttgatt
      421 gcagccaaga tacgcagaat tccgatcctt ccagaaaatg cgcaccggta tcgtatcgag
      481 gaccagctgc agtaacgagt tcagttcggc agtccgtttc cggttcgctt cgatctcgcg
      541 acgcgtctgc ctttcactgg tcacgtccat cccgtagatc gccagggcgt caacccgacc
      601 atcgctcccc gtcaccggat agatcgagtt caagatcgtt cgtccgaacc gctcatcttc
      661 aaaatgcacc ggggaagcgg tttcgatgac ctgctgagca tactttcgac gctgctcggc
      721 aaccgcaggc ggcagcagat catacacatt ggtcttcgcc aactggtcgc gatcgatctg
      781 caagcgcttg agcatgacgt cgttggtctc caggactcga ccttcgacat ccatcaggaa
      841 agccgactcg gaaatggcgt caagaagtgc gcgcacccga cgttcactgg tacgccgcag
      901 ttcttcattc tgaacgcgtg cggtgacatc ggtcgtgaga tgaacaaaac ccgtgatttc
      961 tctggtgggg ctgagaagcg ggtctacggt cacctcaagc cagcgcctgc cgacttgaat
     1021 gaccgcagac tcgcgttgtt tcgtttgacg agcgcgtcta atcgggcacg tcgcgatcgg
     1081 ttcttcggtg ccatgcatca cctgccagca gcggcgaccg agcggggatt cgttggcacc
     1141 aggcacgacg tcgtgcgtcg agcgattggt catgtgaatg cggcacgcgg catccagtac
     1201 ccagatcgtg ctgccgaccg cgtcaataac cgcctgccag ttctgaaacg gcggcgaatt
     1261 ttctagcgcc agtggttcgg ccggcacagg cgactcaggg cggcacgccg tcagttgctg
     1321 tcgcagctcg gccagttcag ccagcaactc ttcgcggctc ttgtcggcgt ctggtcctct
     1381 cgccacgcca agtctctccc ttgtctgatg tttccgggcg gctggagaac aatctgcccg
     1441 aagagcattc ctcgtctgtc gcgaaacata gcacgaaagc aattatcccg acaatggttc
     1501 cgcgtttccc gcctcagagc ggcgcggtat gactgtcagt cgttgaacag acgaagctgc
     1561 gcgtcgtttc gcgagggttc cggcggcctt ttcccgaggt attcgtaggc cagtcgagtt
     1621 gcaatacgac cacggcttgt tcggctcaga aagccgattt gaatcaagaa cgactcgtac
     1681 acatcgtaaa tggtgtctgc ttcttcgccg accgagaccg agagagtatc gagactgacc
     1741 ggcccgccat cgaaccggtt caccagcacg tcgagaatgc ggcggtcgat gtagtcgagt
     1801 ccgagtcggt cgactttgag catctccatg cctcgctgca cgacatccat ctcgatggtt
     1861 tcccgcctgt cgacctgggc gacatcgcgc atgcgcttga acagccgaat ggccactcgc
     1921 ggcgttccgc gtgagcgcct ggcaatctcg cctgcggctt cgccggagat gggatacgag
     1981 aggcggcggg caacagcctg cagcagatcc gtcagttcct caagcgaata aaaatcgatg
     2041 cgcaactgca cgccgaatcg cccacgcaac ggtgcggcga catcgccctc acgcgtcgtc
     2101 gcgcccacca gcgtaaaagg tttcacctgc atgcgaatgc tgcgcgccgt cggacctttg
     2161 ccgatcaaaa catcgaagtg atagtcttcc attgctggat agagaacctc ttcgacggtc
     2221 ttgctgaggc ggtgaatctc atcaatgaac aggacgctgc cggcctgcaa ccccgtcaac
     2281 agagccgcga cgtcacccgc gcggttcagg accgggcccg acgtaatctt gatcggcact
     2341 cccatttcgg cggcaatgac attggcgagc gtggtctttc ccaggcccgg gggtccgagg
     2401 agcaatgtgt gatcgagcgg ttcagatctc tgtctggcgg cctcgataaa gatcttcagt
     2461 ttgtcgacga tgttccgttg tccgatgaat tcctggaaac gacgcggacg cagcgccgga
     2521 gccacgactt cttcggtttc ttcgtccggg tgcgcatgag gtgaaaggat ggctccggca
     2581 gactctgccg ccagcggaac tcccggctct tcgctccgct ctccggagcg cagctcctca
     2641 tcggagtcag aggcgagcgg gggtttctgg cgtttcatga cgttgttggt ttctacttcg
     2701 aaaagtactg gagcacgcgc tcgagcattg cggtgagatc ggcatctaag gccagatcat
     2761 ttgccagatc acgaatggcc cggtcgatct cgcgacccgt gtatcccagg ttctcgagag
     2821 cctcgcgcag gtctttctcg tgcggcaccg cgccgcgccg ggccaccggt ttggccgctt
     2881 catcctcggc gagaaacccg ctcttcagca gttcgtcctt caactcgacc acgaggcgcg
     2941 atgcgatctt ggctccgacc cccttcaggg cggtcagacc ggtcacatct tcgcgcagaa
     3001 tcatgccgac caggcccgct ggcgtcgaag cggacagaat gttcaccgcc agtttcggac
     3061 caaccttgtt gacccgcatc aatagacgaa agatctgccg ttccgcctcg gtggaaaagc
     3121 cgaagagttg cggtccgtcc tcacgccaga ccagatggca cagcagacga gcaccgctgc
     3181 cgacttc
//
LOCUS       DAORHD010000487         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34553_length_3183_cov_4.109974, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000487 DAORHD010000000
VERSION     DAORHD010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34553_length_3183_cov_4.109974"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2431
                     /gene="uvrA"
                     /locus_tag="PKO06_10890"
     CDS             <1..2431
                     /gene="uvrA"
                     /locus_tag="PKO06_10890"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV70195.1"
                     /translation="TPQEILDVLAARPAGTKLTLLAPIVRGRKGEYAKVFDELRKEGF
                     SRVRIDGNLYSLEDDLPALDKKKKHSIDIALDRITLGGSGDRARLLDGIELAFKIADG
                     QLLVLSDDREELFSEKLRCPACEISLPEVKPRLFSFNSPFGACQRCSGLGFQLIVAPE
                     LVMPDTSKSINAGAIAAINAGEETFFRQWLNSLARHFGFSLDVPVEKLPESVQKMILH
                     GSGTEEIAVQYEGKRMQATFKKPFEGIIPMIERRFQETRSEDARTYYEKFMRELPCPD
                     CTGKRLSPAALSVLIEGLNIHELTQKSVGDLVPFFQDLSLTDREAFICKKVLEQIRNR
                     LGFLKDVGLDYLNLSRAAHTLSGGEAQRIRLATQIGSALVGITYVLDEPSIGLHARDN
                     ERLINTLLKLRDIGNTVLVVEHDREIMECCDHLLDLGPGAGREGGHLVAEGPPSDVAR
                     NDASLTGRYLAGKEIIGIPDRRRKPGRRSITLHGASHNNLKQIDVDFPLGMLVAVTGV
                     SGSGKSSLITDTLFPLLSNLMNKSRLDTGSYRKITGTDLIDKVVNIDQNPIGRTPRSN
                     PATYTGLFAPIRDLFAQTIEAKARGFGPGRFSFNVKGGRCEICEGSGQIQIEMHFLPD
                     VYVLCEACKGKRFNDETLKITFKGKHIADVLDMPVKDAVGFFEAFTAIRRKLQTLVEV
                     GLGYITLGQASTTLSGGEAQRIKLATELSRVATGDTFYLLDEPTTGLHFDDVRKLLAV
                     LHRLVDKGNTVVVIEHNLDVIKTADWIIDLGPEGGDRGGEIVAQGTPEQICRIPHSLT
                     GRYLKDYLKDL"
     gene            2434..>3183
                     /locus_tag="PKO06_10895"
     CDS             2434..>3183
                     /locus_tag="PKO06_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNV70196.1"
                     /translation="MAKKTGTLFVCQQCGQSHSKWQGQCDGCKEWNTLAAETRPHAND
                     RQVPRFQSGTANLSLCQSLDNIKGDQAPRLPSGLDLFDDLMGGGLVPGGITLIGGEPG
                     VGKSTFMLQLASRLAVTLKPIVYVSGEESATQIKLRADRLRVGNESDIFLISEQNLEN
                     ALGCCQSYLPRLLIIDSIQTVYTPQIEASPGSVSQVRESAALLTKFGKDRGLPVMIIG
                     HVTKEGAIAGPKVLEHIVDTVLYFEGDISSQF"
BASE COUNT          719 a          925 c          885 g          654 t
ORIGIN      
        1 gactccccag gaaatcctgg atgtcctcgc cgcgcgaccg gcaggaacca agttgactct
       61 gctcgcgcca atcgtgcgcg ggcgtaaagg cgagtatgcc aaagtattcg acgaactacg
      121 aaaagaaggt ttttcgcgag ttcgcattga tggcaacctg tactctcttg aagatgacct
      181 tcccgctctc gacaagaaga agaaacattc gatcgacatc gcactcgacc gcatcaccct
      241 cgggggcagc ggcgatcggg cacggctgtt ggacggcata gaactggcat tcaagatcgc
      301 ggatgggcaa ctgctggttc tgtccgatga ccgcgaggaa ctgttcagcg agaagctgcg
      361 ctgccctgcc tgcgagatca gtcttcccga ggtcaagcca cgcctgtttt ccttcaactc
      421 cccattcgga gcctgtcaac ggtgcagcgg gctcggcttc caactgatcg tcgctccaga
      481 actggtcatg ccagatacgt cgaaatcgat caacgcagga gcgattgccg ccatcaacgc
      541 cggggaagaa accttctttc gacagtggct caacagcctg gcccggcact tcggcttttc
      601 gttggacgta ccagtcgaaa aattgcccga gagcgtgcag aaaatgattc tgcatggttc
      661 aggtacggaa gagatcgcgg tgcagtatga gggcaaacga atgcaggcca cattcaagaa
      721 accttttgaa ggcatcattc ccatgatcga gcgtcgcttt caggagacgc gctcggaaga
      781 tgcacgcacc tactacgaaa agttcatgcg cgagttgcca tgcccggatt gtaccggcaa
      841 gcggctatct ccagcagctc tatcagtgct tatcgaaggt ttaaacattc acgagctgac
      901 tcagaagtca gttggcgatc tggttccgtt ctttcaggat ctgtctctga ccgatcgcga
      961 agccttcatc tgcaagaagg ttctcgagca gattcgcaac cgccttggtt ttctcaagga
     1021 tgtcggactc gactacctga atctgtcacg cgccgcccat acgctctctg gcggggaagc
     1081 ccagcgcatt cggctcgcca cgcagatcgg ttcggccctg gtgggcatca cctatgtcct
     1141 cgacgaaccc agcatcggcc tgcacgcccg cgacaacgag cgcttgatca atacgttgct
     1201 caaactgcgc gacatcggca atactgtgtt ggtggtggaa cacgatcggg aaatcatgga
     1261 gtgctgcgat catctgctcg atcttggccc cggcgccggc cgggaaggcg gacacctggt
     1321 ggcggagggg cctccgtctg atgtggctcg caatgacgcc agtctgaccg ggcgctacct
     1381 tgccggcaaa gaaatcatcg gtattccgga ccgccgccgc aaacccggcc ggcgctccat
     1441 cacgcttcat ggggcttcgc acaacaatct caagcagatc gacgtggatt tcccactcgg
     1501 aatgctggtt gccgtaaccg gagtctcggg atcgggaaag tcaagtctga tcaccgatac
     1561 gctttttccg ctgttatcga atctcatgaa caaatcacgc ctcgacaccg gttcttaccg
     1621 taagatcact gggacagatc tgatcgacaa ggtcgtgaac attgatcaaa atccgatcgg
     1681 acgcacacca cgttcgaatc cggccactta caccggcctg ttcgctccga ttcgcgacct
     1741 ctttgcccag accattgaag cgaaagcacg cggtttcgga cccggacgat tcagtttcaa
     1801 cgtgaaaggt ggacgctgcg agatttgtga aggttcaggt cagattcaga tcgagatgca
     1861 ttttcttccc gacgtgtatg tgctgtgtga agcctgcaag ggcaagcgtt tcaacgacga
     1921 gactctgaaa atcaccttca aaggcaagca catcgctgac gttctcgaca tgccggtcaa
     1981 agatgccgtc ggattcttcg aagccttcac ggccattcga cgcaaactgc agacgctggt
     2041 cgaggtcggg ctcggctaca tcacgctcgg ccaagccagt accacccttt cgggtggcga
     2101 agcacagcgc atcaaactcg ccaccgaact gtcacgcgtg gccaccggag acaccttcta
     2161 tctgctcgat gaaccgacca ccggcctgca cttcgatgac gtccgcaagc tgctggcggt
     2221 actccatcgc cttgtcgaca agggcaacac ggtcgtcgtc atcgaacaca atcttgacgt
     2281 catcaagacc gccgactgga tcatcgacct cggccccgag ggcggtgacc gtgggggtga
     2341 aatcgtagcg caggggactc cggaacagat ctgtcggata ccgcactcgc tcaccggaag
     2401 atatctgaaa gactatctca aggatctctg accatggcca agaaaacggg aaccctgttc
     2461 gtctgtcagc agtgcgggca atcacactcg aagtggcagg gacaatgtga tggctgcaag
     2521 gaatggaaca cgctggccgc cgaaacccgt ccgcatgcca acgatcgtca ggttccccgc
     2581 tttcaatcgg gaacggcaaa tctgtctctg tgtcagtcac tcgacaacat caagggagat
     2641 caggctcccc gactgcccag tggtctggat ctgttcgatg atctgatggg cgggggtctc
     2701 gtgccgggtg gcatcacgct gatcggcgga gaacccgggg ttggcaaatc gacgttcatg
     2761 ctgcagctgg cttcacgcct ggcagtgacg ctcaagccaa tcgtgtatgt ctcgggagaa
     2821 gaatcggcaa cacagatcaa gctgcgtgct gaccggcttc gcgtcggcaa cgaatcggac
     2881 atttttctga tctcggagca gaatctcgag aacgcgctcg gttgctgtca gagttatctg
     2941 ccgcgattgt tgatcatcga ctcgattcaa acggtctaca cgccgcagat cgaggcatca
     3001 ccgggatccg tgtcacaggt gcgcgaaagc gcggcattgc tcaccaagtt cggaaaagac
     3061 cgcgggctgc cggtcatgat catcgggcac gtcaccaagg aaggagccat tgcgggaccc
     3121 aaggtactcg agcacatcgt cgacacggtg ttgtatttcg aaggcgacat ctcatcgcag
     3181 ttc
//
LOCUS       DAORHD010000488         3175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34701_length_3175_cov_3.245833, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000488 DAORHD010000000
VERSION     DAORHD010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34701_length_3175_cov_3.245833"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKO06_10900"
     CDS             <1..599
                     /locus_tag="PKO06_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV70197.1"
                     /translation="RNENSYGHIITIEDPIEYSFMGYKSSIISQRQLGEDTPSFHDSL
                     TSSLRQDPDVIFIGELRDQETVETCLKAAETGHLILGTLHTANAVQSILRLINIFPSH
                     QRDNIRFMLSSCLKMVLSQALIPLKTRKGRALAYEVLPMLPSVCNLIRQKKIHEINSV
                     LRTSRRSGCIAMSATIKELLKTGLVDQVDVPNELREEK"
     gene            603..2003
                     /locus_tag="PKO06_10905"
     CDS             603..2003
                     /locus_tag="PKO06_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70198.1"
                     /translation="MGLFDSLGGGAAQKELEEAKKEIAALKDQLRKATIGRDEAQKKN
                     VHLIDKTHSFLQRLRLLAGTLEDQNVIPRAWDLLDMTLTIKKGGFFERTPEGWKTSFS
                     VGWPENAAPVIPLAEESMATYCADHGVPLSIAHLRKQDDLAFLERRGIIPDTKIVCPI
                     RVRGKVQKLLLICNYGGNVFASEDDLDTLEIVTTLLGLVMTNAMILLEQKKELFEKSI
                     ALSRLRNIFSHLVAPEVIEYLEKNPDGVVLGGKRQPVVIMFADIRGFTNISENLSPER
                     VIDLLNRYFSRLTDIILTHKGTLDKFMGDAAMVLFGTPMPIDNPVERAAAAAQEIQDM
                     VQDEMPEWVKAGLPPFSVGIGINYQEVVVGNVGSQKLSNFTVIGDGVNLAFRLCSVAQ
                     GGEILVSESVYESLQAWEGKVEARPKIHLKGKAEPVTVYALSRYSNDRAGLCPRCAQP
                     VGEGVRFCANCGFRRF"
     gene            2117..>3175
                     /locus_tag="PKO06_10910"
     CDS             2117..>3175
                     /locus_tag="PKO06_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70199.1"
                     /translation="MRTAIVCCALIACVAVLSITGCGTIVTSSPPVPAAVALRPKVAS
                     GTGSISGSYFPVDLRNVARTADGRIRAKNPAAGREVQVGYYTKDGRFSIQQASSIASD
                     GAFLISNVPATHRNLIVQVALGTDTPTAVVPCIEVGKTVTCMPFDRNSSVLAYFVREA
                     CLQDAADNLSLLELLVRLPSAELKDLAREPDALRKALSVFLEREQTIDRMARIIGEGT
                     IAELRQWEAEHVNALNRLMTSGLLPDKHDWRLSAYAREVKWQALSLKPWQRMLFHQVE
                     RQIVQDKLNRMQFVSSFDEMTFAQEEPAFEFCSASLLEACAFFEKKKWLSPADVEPLK
                     NFLKTVRPYLRRAKEAHMI"
BASE COUNT          713 a          952 c          859 g          651 t
ORIGIN      
        1 accgcaatga gaattcgtac ggtcacatca tcaccatcga agatccgatc gagtactcgt
       61 tcatggggta caaatcgtcg atcatcagcc agcgacagct tggtgaagac acgccgtcgt
      121 tccacgacag tctgacatcc tcgttacgcc aagaccccga tgtcattttc atcggtgaac
      181 tgcgcgatca ggaaaccgtc gaaacctgtc tgaaagcagc cgaaacgggg catctgattc
      241 tcggcaccct gcataccgct aacgccgtac agagtattct gcgcctcatc aacatcttcc
      301 cgtcgcatca gcgcgataac attcgtttca tgctctcgag ctgtctgaag atggtgctgt
      361 cgcaggcgct gataccgttg aaaacccgca aggggcgcgc gcttgcgtat gaagtgctgc
      421 ccatgttgcc atcggtctgc aatctgattc gccagaaaaa aattcacgaa atcaattcgg
      481 ttctgcgcac cagtcggaga tccgggtgta tcgcaatgtc cgcaaccatc aaggaactgc
      541 tgaagaccgg gctcgtcgat caggtcgacg tgcccaacga gctgcgagag gaaaagtaac
      601 gtatgggcct gttcgattcc cttgggggcg gagcggcgca gaaagagctc gaagaggcaa
      661 agaaagagat cgccgcgctg aaggatcagc tgcgcaaggc cacgatcggt cgcgacgaag
      721 cgcagaaaaa gaacgttcac ctgatcgaca agacccactc cttcctgcag cgactgcggt
      781 tgctcgccgg aacgctcgaa gaccagaatg tcattccccg cgcgtgggac cttctggaca
      841 tgaccctgac gatcaagaag ggcggcttct tcgaacgcac acccgaaggc tggaaaacga
      901 gcttcagtgt cggctggccc gagaacgccg cccccgtcat tccgctcgcg gaagaatcga
      961 tggcgaccta ctgcgccgac cacggggtcc cgctcagcat cgctcatctg cgcaaacagg
     1021 atgatctcgc ctttctcgaa cggcgcggca tcattcccga caccaagatc gtctgcccga
     1081 ttcgggtccg cggcaaagtg cagaaactgc tgttgatctg caattacggc ggcaacgttt
     1141 tcgccagcga agacgatctc gacacgctcg aaatcgtgac cacgctgctc ggactggtca
     1201 tgaccaacgc catgattctg ctggaacaga agaaagaatt gttcgaaaaa tcgatcgctc
     1261 tgtcgcgcct gcgtaacatc ttctcgcatc tcgtcgctcc cgaagtcatc gaatacctcg
     1321 agaagaaccc tgacggcgtg gtgctcggcg gcaaacgcca accggtcgtc atcatgttcg
     1381 ccgacattcg cggcttcaca aacatctccg aaaatctgtc tccagagcgg gtcatcgacc
     1441 tgctcaaccg gtacttttca cgattgaccg atatcattct cacgcacaag ggcaccctcg
     1501 acaaattcat gggcgatgcc gcgatggtgt tgttcggcac gccgatgccg atcgacaacc
     1561 ccgtcgaaag agctgcggca gcggctcaag agattcagga catggtacag gacgaaatgc
     1621 ccgagtgggt gaaagctggt ctgccgccat tttccgtcgg catcggcatc aactatcagg
     1681 aagtcgtcgt cggcaacgtc gggtcgcaga agctctcgaa cttcaccgtt attggtgacg
     1741 gcgtcaacct ggcttttcga ctctgttcgg tcgcccaggg cggcgagatt ctcgtgtctg
     1801 agtcggtgta tgaatcgctg caggcgtggg aaggcaaagt cgaagcccgc ccgaagattc
     1861 atctcaaggg caaggccgaa ccggtcaccg tttacgctct ctcgcgctac agcaatgatc
     1921 gcgccgggct ctgcccccgc tgcgcccaac ccgttggtga aggcgtgcgc ttctgtgcca
     1981 actgcggttt ccgccgcttc tagcgtcata cttcgacaga gccccccccg atcgcggcaa
     2041 ccgagacctc gtcgcctgtc gcgacgatac gctcatcacc cctcatgaaa tgtgcattcc
     2101 tggaaggaaa ccccaagtga gaaccgccat cgtctgctgc gctctgatcg cctgcgtcgc
     2161 cgttctttcg atcaccggat gcggcaccat cgtcacctcc tctccacccg tgccggccgc
     2221 ggtggccttg cgcccgaagg ttgcgtcagg gacaggaagc atctctggca gttactttcc
     2281 tgtcgatctg agaaatgtgg cccgaacggc ggatggccgc atacgtgcga aaaacccggc
     2341 cgccggaaga gaagtacaag ttggttatta caccaaggac ggtcgtttca gcattcagca
     2401 ggcatcgagt atcgcctcag acggcgcctt tctcatcagc aatgtcccag ccacgcatcg
     2461 caatctgatt gtccaggttg cactcggaac cgacacgcct acagccgtag ttccgtgcat
     2521 agaggttggc aagacggtca cgtgcatgcc cttcgatcgc aactcatctg tactggcata
     2581 cttcgttcgt gaagcttgtc tgcaagatgc agcggacaac ctgagccttc tcgaactcct
     2641 cgtgcgactg ccctctgccg aactgaagga tctggccagg gaaccagatg cgttgagaaa
     2701 ggcgttgagc gtttttctcg agcgtgagca aaccatcgac cgcatggccc gtatcatcgg
     2761 cgaaggaacg atagccgagc tacgtcagtg ggaggcggaa cacgtgaatg ctctgaaccg
     2821 actcatgacg agtggcctcc ttccagacaa gcatgactgg cgcctttctg cctacgcccg
     2881 tgaagtgaaa tggcaagcgc tttccctcaa gccctggcag cgcatgctgt ttcatcaggt
     2941 cgaacgtcag atcgttcagg acaaactgaa tcgcatgcag ttcgtgtcct ccttcgatga
     3001 aatgacgttt gcccaggaag aacccgcctt tgagttttgc tctgcatcac tgctcgaagc
     3061 gtgtgcgttt ttcgagaaga aaaagtggct ttcgccggcg gatgtcgagc ccctgaagaa
     3121 ctttctgaag accgttcgac cgtatctgcg acgagccaaa gaagcgcaca tgatt
//
LOCUS       DAORHD010000489         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34741_length_3172_cov_2.942573, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000489 DAORHD010000000
VERSION     DAORHD010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34741_length_3172_cov_2.942573"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PKO06_10915"
     CDS             complement(<1..1960)
                     /locus_tag="PKO06_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNV70200.1"
                     /translation="MAKCPKCSRDLPLSGRCPFCDKATAGGGFSLKTFAAKGGEAPPT
                     KPSPAPDPAPAPLASGLPSLDDVDPPAPPPPAPQASSLPPTPPLATATPKPASSSILD
                     SLRGRPTAPPPAPSAPPPVSPADLASAPTVATPISTPIVAPASSNLTSRSAAQPEASI
                     LSQIKSKNETERKAAIEAPLKDDAVPNTSIYSSPLTGFGDEEMECSVLAGILSDQYTL
                     ESSLSKGLMVDLFSTKYSRALAKAVLMLREENREGSVIDKIILKNKLRAQGQYDTPMQ
                     NFLNRVAAMPAPTLDQVLHYMELLKEGALKRKLKEIANRIVEYIEVPADNKPGLENFS
                     TAIVRDIREVQKAKTSKRINLVKEEMREIIKDMDRREIEGETEIIGYSFEPFHYLNIA
                     LSGFRKGFLYAIAGAPRRGKTTFTLELATSVATNCKIPVLFFTYEQTKKNLTYRLLAK
                     ESRVNPDTLQRKKIRSDDIAQAKFMGGWKKMQNYMDYFYIIEATKVDTVDRIRTYAYN
                     VMQDFNTENIMIFVDYIQKMPLSRQYMDEKFKVEEISTELKGLTIELNNPLMVISSLN
                     KEGCLIDGTDSPERPTMYHCKGSGDLEYDLDAAMIQAKDWGDTKELYAQLQQKAEELG
                     KDPTRVPKVEVVNLYLDKNRDAPEGIYSI"
     gene            2195..>3172
                     /locus_tag="PKO06_10920"
     CDS             2195..>3172
                     /locus_tag="PKO06_10920"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNV70201.1"
                     /translation="MPTSVRRLIPLLISLLAVACWAGAYESNIVQGFKVEGNRLISEE
                     VILLNVKLKPGDRLTNDAVQAEIARIGEMGFFSYVGAEVRPSEKGKTVVFKVEENAIV
                     GEIEFKGTTKVPSEKIAKAMETRTGTVFNSKLLNQDIQTVNEYLAKEGYMFNKVVDVQ
                     VKDKGSKIFIEIVEAKLSDIKIEGLKKTKEKVVRRELTCKAGEIYDNNKIVRDLQRIY
                     NLGFFEEVKRDHLPGKNPDEIVLVIQVVEQRTGRAGVGGGYSSLNGLVGFANLSQNNW
                     QGKGQRAYVKSEFGGVNLWQVGFFDPWFRDKPQSFGVDLYNTKYTRNLYL"
BASE COUNT          680 a          793 c          934 g          765 t
ORIGIN      
        1 tgattgagta aatgccttcc ggggcatcgc ggttcttgtc gagatacagg ttgaccactt
       61 cgaccttggg aacgcgggtc ggatccttgc cgagttcttc ggccttctgc tgcagctggg
      121 catacaactc cttggtatcg ccccagtctt ttgcctggat catcgcggcg tcaagatcgt
      181 attccaggtc tcctgacccc ttgcagtgat acatggtcgg gcgttcgggt gaatccgtac
      241 cgtcgatgag acacccttcc ttgttcagag agctgataac catgagtgga ttattgagtt
      301 cgatggtgag acccttcagt tccgtcgaga tttcttcgac cttgaacttt tcatccatat
      361 actgacgcga caacggcatc ttctggatgt aatccacgaa gatcatgatg ttttccgtat
      421 tgaagtcctg catgacattg tacgcatatg tcctgattcg gtcgacggtg tccaccttcg
      481 tggcttcgat aatgtagaaa tagtccatgt aattctgcat cttcttccag ccgcccatga
      541 acttggcctg ggcgatatcg tccgagcgga tcttcttgcg ctgcaaggtg tcaggattga
      601 cgcggctttc cttggccagc aggcggtacg tcaggttctt tttggtctgc tcatacgtga
      661 agaacaaaac cggaattttg cagttggtcg cgaccgacgt ggccagctca agtgtgaatg
      721 tcgtcttccc gcgccgcggt gcgccggcga tagcatacag aaagcctttg cgaaagccgg
      781 aaagcgcgat gttcagatag tgaaacggct cgaacgagta gccgatgatt tcagtctcgc
      841 cttcgatttc gcggcgatcc atatccttga tgatttcgcg catctcttct ttgaccaggt
      901 tgatgcgctt ggaggtcttg gccttctgta cttcgcgaat gtcgcggaca atcgccgtcg
      961 agaaattctc gagacccggc ttgttgtcag ccggaacctc gatgtactcg acaattcgat
     1021 tcgcgatttc cttgagtttg cgcttgaggg ccccttcctt gagcagctcc atgtagtgca
     1081 ggacctgatc gagggtagga gcaggcatgg ccgcgacacg attcaggaaa ttctgcatcg
     1141 gcgtgtcata ctggccttgc gcgcgcagtt tgtttttgag aatgatcttg tcgatgaccg
     1201 agccctcgcg attctcctcg cgaagcatga gaacagcctt ggccagcgcg cgtgaatact
     1261 tggtcgaaaa caggtcgacc atcagacctt tgctgagaga agactccaac gtgtattggt
     1321 cgctcagaat gccggcgagc accgaacatt ccatctcttc gtcaccgaaa ccggtgaggg
     1381 gggaactgta gatcgaggtg ttggggaccg cgtcgtcctt gagcggagct tcgattgccg
     1441 ctttgcgttc ggtctcattt ttcgatttga tctgcgagag aatcgacgcc tctggttggg
     1501 cggccgaccg agaagtgaga ttcgaggatg cgggcgcgac aatcggggtt gagatcggtg
     1561 tggcgacggt tggcgctgag gcgagatcgg ccgggctcac gggaggtggc gcagatgggg
     1621 ctggcggcgg tgcagtcggg cgaccgcgca acgaatcgag aatcgaactg gatgccggtt
     1681 ttggggtggc cgtcgccaga ggaggggtgg gtggaagcga tgaggcctgc ggtgcgggcg
     1741 gaggcggtgc cggtggatcg acatcgtcaa gtgaggggag tcctgatgct agaggcgctg
     1801 gcgcagggtc tggagccggt gagggtttcg tgggaggggc ctcaccaccc ttggcagcaa
     1861 aggtcttgag cgagaacccg ccacctgcgg tggctttgtc gcaaaacgga caacgtccgc
     1921 tgagggggag gtcccgactg catttggggc atttggccat gactctgctc cttcgacgag
     1981 agaactcgac aacgatcaat ccggttgcca ttgtagccgt ttttgtgccc ggaaaaaagt
     2041 cctggaagtt tacatgaaat atgtttatgt ttgttcgtcg acattgtgtc cgttccgcca
     2101 aggtccgttc cggttccttg acaacctaag ggtcaccctt tatcatgccg ataccttttc
     2161 agacccttcc tgaatgggag aaataaccat tattatgcca acatctgtcc gccgtttgat
     2221 tccgctgttg atcagccttc ttgctgttgc ctgctgggcc ggtgcctacg agagcaacat
     2281 cgtacagggt ttcaaggttg agggaaaccg cctcatttcc gaagaggtca ttctcctcaa
     2341 cgtcaaactg aaacccggag atcgactgac caacgatgcc gtgcaggctg agattgcccg
     2401 cattggcgag atgggcttct tctcttacgt cggagccgaa gtgcgtcctt ccgaaaaagg
     2461 aaagacggtt gtgttcaagg tcgaggagaa cgccattgtc ggcgagatcg agttcaaggg
     2521 aacgaccaag gtgccgagcg agaaaatcgc caaagccatg gaaacccgca ctggaactgt
     2581 cttcaactcg aagctcctga accaggacat tcagaccgtc aacgagtacc tcgccaaaga
     2641 gggctacatg ttcaataagg tcgtcgatgt ccaggtcaag gacaaaggca gcaagatctt
     2701 cattgaaatt gttgaagcca aactttctga tatcaagatc gaaggcttga agaagaccaa
     2761 ggaaaaagtg gtgcggcgtg aattgacctg caaagccggt gaaatttacg acaacaacaa
     2821 gatcgttcga gatctgcagc gcatttacaa cctgggtttc ttcgaagaag tgaaacgcga
     2881 tcacctgccg gggaaaaacc ctgacgaaat cgttctggtc attcaggttg tcgaacagcg
     2941 caccggtcgg gctggggtcg gcggtggcta cagttcgttg aacggtctgg tcggctttgc
     3001 caacctgtcg cagaacaact ggcagggcaa aggacagcga gcgtatgtga aatccgagtt
     3061 tggtggcgtc aatttgtggc aggtcgggtt ctttgatccc tggttccgcg ataagccgca
     3121 gagtttcggc gtcgatttgt acaatacgaa gtatacccgc aacctctatc tg
//
LOCUS       DAORHD010000490         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34761_length_3171_cov_3.032413, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000490 DAORHD010000000
VERSION     DAORHD010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34761_length_3171_cov_3.032413"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PKO06_10925"
     CDS             complement(<1..624)
                     /locus_tag="PKO06_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV70202.1"
                     /translation="MRTFLIGVLIFWGVTWGLIVQNDSQRERDTGRDVNDGRVEVIFW
                     HAMSGPLGKVMDRLTDRYNASQKTYFIKSICMGNYDTLQKKLLASIVARQAPDIAQNY
                     ESVTRKFAKYGKIVCIDDLLASETEDIRADIVPVLLANNTFDSKLWSFPFNKSVPVLY
                     YNKDLFKKAGLDPERPPKTWDDMLTYAEKLTQRDEQGRTTVYGFGTGK"
     gene            complement(634..1614)
                     /locus_tag="PKO06_10930"
     CDS             complement(634..1614)
                     /locus_tag="PKO06_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV70203.1"
                     /translation="MKHLMKNPFHLVIHFFLILGSIFMLFPFLWMIFTSFKNEDEILK
                     TGKQIILLPDSMRPGFLNDDKARERLAEQEKLEAAWVAAHSQAEVASSSQPLNKLKKP
                     FHFWDNYREALAAQPFYRYFLNTVFVSISITAGSLFLASLAAYAFAFFTFPFKDLLFT
                     LMLGTLMIPQQALLIPNYIILSKMGWINTFAAMIVPWLASAYSVFFLRQFFLQLPKDL
                     YDAAVIDGCSKFQFYYKILLPLAKPPMVTLGIFSFLGTWNSFVWPLIVTNDTSLRVIQ
                     VGLSYFSSEAGTRWGPLMAASTMTIVPLVIMYFIAQRQFVESQAMSGMKE"
     gene            complement(1622..2503)
                     /locus_tag="PKO06_10935"
     CDS             complement(1622..2503)
                     /locus_tag="PKO06_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014599396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNV70204.1"
                     /translation="MAAENKRSDVSSYGYLVPVFLILLTFQVFPIFYSFGLSLYSWDL
                     IGEPEWLGLDNFWRLWDDPMFWKSIWNTMYYSVLIVPLSIFCSLSVAMLLNNPIAGLG
                     IYRTIYFLPVITSINAVAIVWNFIYHPDYGLLNKLLAIVGLPAQTWLQDPFWAMPCII
                     LMSVWKGLGYNVIIFLTGLQNIPKHLYEAARIDGASRWQQFRHITWPLLSPTTYFVFT
                     MSIIGSFQVFSQVYMMTPRGGPLKSTSVVVYYLYQKAFEKFEFGYALAMAFVLFVIIF
                     SATMFNKLYVEKKVHYN"
     gene            complement(2575..>3171)
                     /locus_tag="PKO06_10940"
     CDS             complement(2575..>3171)
                     /locus_tag="PKO06_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70205.1"
                     /translation="LTTGDWDSADLAGRGYVNFLLTKLGSVMRLNDDQVWDQTNKTNK
                     PWGVLAHVVKQDHPSMKGVKVVITWGTCSLITRDKQPLTAAAGVDLLITGDDDTTNKD
                     GNKKNDAIIYPKGTPIAIAAEEKLANGILVLIGCSNFTDYQYPDSDINLAKPGPAPFT
                     HETTIFYDQMITYLMGLDQPARSTTPARTNRRSSNRSR"
BASE COUNT          722 a          787 c          992 g          670 t
ORIGIN      
        1 cttgccggtg ccgaaaccat acacggtggt gcgaccctgc tcgtcgcgct gggtcagttt
       61 ctcggcatag gtaagcatgt cgtcccaggt ttttggcggg cgttcggggt cgagccccgc
      121 tttcttgaac agatctttat tgtagtaaag aactggtacg ctcttattga acgggaacga
      181 ccacagcttg ctgtcgaacg tattgttcgc gagcagcacc ggcacgatgt cggcgcgaat
      241 gtcttcggtt tctgaggcca gtagatcatc gatgcagacg atcttgccat acttggcgaa
      301 cttgcgagtg acgctctcgt agttttgcgc gatatccggt gcctgacgcg caacgatcga
      361 tgcaagcagt ttcttctgca gggtgtcgta gttccccatg cagatgctct tgatgaagta
      421 ggttttctgc gaggcattgt accggtcggt caggcggtcc atgaccttgc cgagcgggcc
      481 tgacatggca tgccagaaga tgacttcgac gcgaccgtcg ttaacgtcgc gaccggtatc
      541 ccgttcgcgc tgactgtcat tctgcacgat cagaccccag gtcacgcccc agaagatgag
      601 aacgccgatg agaaaagtac gcatatcggc ctcctattcc ttcatgcccg acatggcctg
      661 agattcgacg aactgacgct gggcgatgaa atacatgatg accagcggca cgatcgtcat
      721 ggtcgacgcg gccatgagcg gaccccagcg ggttccggcc tcgctcgaaa agtaactgag
      781 gccgacctga atgacgcgca ggctggtgtc gttggtgacg atgagcggcc agacgaagct
      841 gttccaggtg ccgagaaacg agaagatgcc gagcgtgacc atcggcggct tggccagcgg
      901 cagcagaatc ttgtaataga actgaaactt gctgcaacca tcgataacgg cggcatcgta
      961 caagtctttg ggtagctgca gaaagaattg gcgcagaaag aagaccgaat acgccgaagc
     1021 cagccacggc acgatcatcg ccgcgaacgt gttgatccag cccatcttcg acagaatgat
     1081 gtagtttgga atcagcagcg cctgctgcgg aatcatcagt gtgcccagca tcagcgtgaa
     1141 cagcagatct ttaaagggga aggtgaaaaa ggcgaacgca taggctgcca gcgaggcgag
     1201 aaagagagag cccgcggtga tcgagatcga cacgaagacc gtgttcagaa agtagcggta
     1261 gaagggttgg gcggcgaggg cttcgcgata gttgtcccag aaatgaaagg gcttcttcag
     1321 cttgttgaga ggctgagatg aggatgcgac ttcggcctga ctgtgcgcgg caacccaggc
     1381 ggcttccagt ttttcctgct cggcgaggcg ttcacgtgcc ttgtcgtcgt tcagaaagcc
     1441 cgggcgcatc gagtcgggca gcagaatgat ctgtttgcca gtcttgagaa tctcgtcctc
     1501 gttcttgaac gacgtgaaga tcatccagag gaaggggaag agcatgaaga tcgagcccag
     1561 aatcagaaag aaatggatga cgagatggaa ggggtttttc atcaggtgtt tcatgtcatc
     1621 ctcagttgta gtgaactttc ttttcgacgt agagcttatt gaacatggtc gcgctgaaaa
     1681 tgatgacgaa cagaacgaag gccatcgcta acgcgtatcc gaactcgaat ttctcgaaag
     1741 ccttctggta caaatagtag acgacgaccg aggtgctctt gagcgggccg ccgcgtgggg
     1801 tcatcatgta gacctgcgag aacacctgaa aactgccgat gatcgacatc gtaaagacga
     1861 agtaggtcgt aggcgaaagg agtggccagg tgatgtgccg aaactgctgc cagcgcgaag
     1921 caccgtcgat gcgagcggcc tcatatagat gcttgggaat attctgcaac ccggtcagaa
     1981 aaatgatgac gttgtagccc agacctttcc agacgctcat gagaatgata cacggcatgg
     2041 cccagaatgg gtcttgcagc caggtctgcg cgggaaggcc gacgatggca agcagtttgt
     2101 tcagcaggcc gtagtccggg tggtaaatga agttccagac gatggcgacg gcgttgatcg
     2161 aagtgatgac cggcaaaaag tagatggtgc ggtagatgcc cagcccggcg atcgggttgt
     2221 tcagcagcat ggcgacgctg agcgaacaga agatcgacaa cgggacgatc agcaccgagt
     2281 agtacatcgt gttccagatc gacttccaga acattgggtc gtcccagagt cgccagaaat
     2341 tgtccaggcc gagccactcg ggttcgccga tgagatccca gctgtagagg gaaagaccga
     2401 acgaatagaa gatcggaaag acctggaagg tcaacaggat cagaaagacg gggaccagat
     2461 atccgtagga actgacgtcg cttcgtttgt tttcagcggc catgcggtga ttagctcaac
     2521 agaaagggga tgaatcggac aggtccccgg gcgttcgccc ggggaccatg cggtctagcg
     2581 ggaacgattg ctggagcggc ggttcgtgcg tgcgggcgtg gtcgaacgag cgggctgatc
     2641 gaggcccatc agatacgtga tcatctgatc gtagaagatc gtggtttcgt gcgtgaacgg
     2701 tgccgggccg ggcttggcga gattgatgtc gctgtccgga tactggtagt ccgtgaagtt
     2761 cgaacaaccg atcagtacca gaatgccgtt ggccagcttc tcttcggcgg cgatggcgat
     2821 gggcgtgcct ttcggataaa tgatggcgtc attcttcttg ttgccatctt tgttcgtggt
     2881 gtcgtcgtca ccggtgatca gcagatcgac cccggctgcg gcggtgagcg gctgtttgtc
     2941 gcgggtgatc agcgagcagg ttccccacgt gatgacgact ttgacgccct tcatcgacgg
     3001 gtgatcctgc ttgaccacgt gagcgagcac gccccacggt ttgttggttt tgttggtttg
     3061 atcccagacc tggtcgtcgt tcaggcgcat gaccgaaccg agcttggtga gcaggaagtt
     3121 tacatagccg cgtccggcga gatcggcgct gtcccagtcg ccggtggtca g
//
LOCUS       DAORHD010000491         3167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34825_length_3167_cov_3.306234, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000491 DAORHD010000000
VERSION     DAORHD010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34825_length_3167_cov_3.306234"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKO06_10945"
     CDS             complement(<1..246)
                     /locus_tag="PKO06_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNV70206.1"
                     /translation="MSRIFVSGLINLETTLKIESFPLNYFPVCYPFHGIHDRISGVGF
                     NVAKALHTLGDEVCFCSLLGRDHAGAASRSTPRRPACA"
     gene            complement(257..844)
                     /locus_tag="PKO06_10950"
     CDS             complement(257..844)
                     /locus_tag="PKO06_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV70207.1"
                     /translation="MTQPSLCCDLYLIGVGGQGIGLLAESILRAADAAGMPVRGVDTH
                     GLAQRGGTVTSHVRIGNTAHSPLVRPGAAHVVVALERHEALRGMNSHLRDGGTLVYYD
                     CELQPLPVRLGKTGRLDPELVTREAQRRGIRVFRTKLSDLPDPRMQNIAVLAALISHQ
                     VLPGLDRSHFEAALTDLMQGAALDANLALLRRLTT"
     gene            complement(841..2727)
                     /locus_tag="PKO06_10955"
     CDS             complement(841..2727)
                     /locus_tag="PKO06_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV70208.1"
                     /translation="MKKLGELLLASNPAPEITMGNYALVRAMIESGTRVVTSYPGSPT
                     PEIAEAIASLPKEANPLYFEFSTNEKVALEVAFGAAVNGHTSCVFFKSVGLNVAADSF
                     VQLSMMEIIGGMVIILGDDPGANSSQNEQDNRHYARLSYTPLFEPASPTEAYEMFKEA
                     TRISREKRMPVILRLTTHVCHAKEKIAFGPYQPITCDDTPRFDPKNGPYWPITAAVFP
                     LKRKALKRLQELATYAESCRFTRVVDHRNPQRGIITAGLPYYSLLDSLEQAALKPDLL
                     ILGLVHPLPRRRIVEFLRSHVEVKILEELDDFLEAQIKAIAYEEKLSTRILGKLDPDE
                     WLGEYTPERVDEILRRCWADLLPAAPPKAERPALAPVRPPQLCPGCGHRTAFHAVKKA
                     LPPETITVADIGCHSLGFLEPYEMGQVLLCMGHSSGTAAGMSLFNRTRPVVAFIGDST
                     FFHAGLPAQINALFNRHNFTLIVMENGTTAMTGHQNHPASGGNFREAVEAIPIRRVLE
                     ALGVKSIRECDTYQEGKLIEMVKLAIAEPGFKVVIARHPCMLKFTRETRRKGRSLQPP
                     VRVSETCDLKKACIADFACPTYQFGSDGKVFVQTDLCIGDQSCRQNCPTQAIVPSTTT
                     EKPQ"
     gene            2870..>3167
                     /locus_tag="PKO06_10960"
     CDS             2870..>3167
                     /locus_tag="PKO06_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70209.1"
                     /translation="MKHPCVHPRLGLARLAVILCVTVLVIAALVSGYWWLTSKRRVPR
                     PPEPPKPPVSVATASVLVASPSRPIVPATHPVTVRPPVSVWPEPEVVAATREAGG"
BASE COUNT          598 a          892 c         1046 g          631 t
ORIGIN      
        1 tgcacacgct gggcgacgag gtgtgcttct gctcgctgct ccggcgtggt cccgcccgag
       61 cagcgagcag aagcacacct cgtcgcccag cgtgtgcagg gctttggcga cgttgaagcc
      121 gacgccggag atccgatcgt gaattccatg aaatggataa cacaccggaa agtagttgag
      181 cggaaacgac tcgatcttca gagtggtttc gaggttgatc agtccggaaa cgaagatgcg
      241 actcatgcgg acgggctcag gttgtcagac gacgcagcag agccagattg gcgtcgaggg
      301 ctgcaccctg catcagatca gtgagtgcgg cttcgaagtg cgaccggtcg agacccggca
      361 gtacctgatg tgagatcagc gcggccagca ccgcgatgtt ctgcatgcgc ggatcaggca
      421 gatcggagag cttggtgcga aatacccgga taccgcgtcg ttgtgcttcg cgggtgacga
      481 gttccgggtc gagccgacct gtcttgccga gccgcaccgg cagcggttgc agttcgcagt
      541 cgtagtagac gagggtgccg ccatcgcgca gatggctgtt catgcctcgc agagcctcgt
      601 ggcgttcgag ggcgacgacc acgtgcgctg ctccggggcg caccagcggg gaatgcgccg
      661 tattgccgat acggacatgc gaggtcacgg ttccgccgcg ttgcgccagg ccatgggtat
      721 cgaccccacg cacgggcatt ccggccgcgt cggcggcgcg cagaatcgac tcggccagca
      781 gaccgatgcc ctgaccgcct acaccgatga gatacaggtc gcagcagagg gaaggctgag
      841 tcattgcggt ttctccgtgg tggtggatgg gacaatcgcc tgggtcgggc agttctgtcg
      901 gcacgactga tcgccgatgc acagatcggt ttgtacgaag accttgccgt ctgagccaaa
      961 ctggtaggtc gggcaggcga aatcggcgat gcaggccttc ttcaggtcgc aggtctcgct
     1021 gactcgtacc ggcggttgca gcgatcggcc tttgcggcgt gtttcccgcg tgaacttcag
     1081 catgcaggga tgccgtgcga tgacgacctt gaagccgggt tcggcaatgg ccagtttcac
     1141 catctcgatc agtttgcctt cctgatacgt atcgcactcg cgaatactct tgacgcccag
     1201 ggcttcgagc acccgtcgaa tcggaatggc ctcgaccgcc tcacggaagt tgccgcccga
     1261 cgccggatgg ttctgatgcc cggtcatggc ggtggtgccg ttttccatga cgatcagggt
     1321 gaagttgtgg cggttgaaga gcgcgttgat ctgcgccggc agtccggcgt gaaagaaggt
     1381 cgagtcacca atgaaggcca cgacggggcg tgtgcgattg aacagcgaca tgccggcggc
     1441 ggtaccgctc gaatggccca tgcacagcag aacctggccc atctcatagg gttcgagaaa
     1501 gccaagactg tgacagccga tatccgcgac ggtgatggtt tcggggggca gggccttctt
     1561 gacggcatga aaggcggtgc gatggccgca tccagggcag agttgcgggg ggcgtaccgg
     1621 cgcgagggcg ggccgttcgg ctttgggcgg tgccgccggc agcagatcgg cccagcagcg
     1681 tcgcagaatc tcgtcgacgc gctccggcgt gtattcgccc agccattcgt ccgggtcgag
     1741 tttgccgaga atcctggtcg aaagcttctc ttcgtaggcg attgccttga tctgcgcctc
     1801 gagaaagtca tcgagttctt ccagaatctt gacttcgaca tggctccgca gaaactcgac
     1861 gatgcgtcgg cgggggagcg ggtgtaccag tccgagaatg agaagatctg gtttcagagc
     1921 cgcttgttcg agtgagtcga gaagcgagta atacggcagg ccggcggtaa tgattccccg
     1981 ctgggggttg cgatgatcga cgactcgggt gaaacggcac gattccgcat aggtggccag
     2041 ctcctgcagg cgcttcaggg cctttcgctt caggggaaag acggctgcgg tgatcggcca
     2101 gtaggggccg tttttcggat cgaagcgcgg tgtgtcgtcg cacgtgatcg gctgataggg
     2161 gccgaacgcg atcttttcct tggcatgaca gacgtgggtg gtcaggcgca gaatgaccgg
     2221 catgcgtttc tcgcgtgaaa tgcgggtcgc ctctttgaac atttcgtatg cttccgtcgg
     2281 ggaagccggc tcaaacaaag gagtgtagga gaggcgcgcg taatgccggt tgtcctgctc
     2341 gttttgcgac gaattggcac cgggatcatc acccagaata atgaccatgc cgccgatgat
     2401 ttccatcata ctgagttgta caaaagagtc ggcggcaaca ttgaggccga cgcttttaaa
     2461 gaacacgcaa gaggtgtgtc cgttgactgc cgcgccaaac gcgacttcca acgcaacttt
     2521 ttcgttggtg gaaaactcga aatagagcgg atttgcctct ttcggcagcg aggcgatcgc
     2581 ttcggcgatc tcgggagtgg gagaacccgg atacgacgtg acgacgcgcg tgcccgattc
     2641 gatcatggcg cgaacgaggg cgtagttgcc catggtaatc tcgggagccg gattcgacgc
     2701 cagcagaagt tcaccaagtt tcttcataaa accacctttg cggtgttgag tctcggcgag
     2761 cattctaccc tgctctcccc gccacgagca aacccagggc agacagcgat tctgccccat
     2821 gttctctgga gattgaatgc ggagcacgag taggctatgc tgaattgaca tgaaacaccc
     2881 ttgtgttcac cctcgactcg gactggcgcg gctggcggtg atcctctgcg tcaccgtgct
     2941 ggtcatcgcc gcgctggtca gtggatactg gtggttaaca tcgaagcgaa gggtccctcg
     3001 gccccctgaa ccgcccaaac cgccggtctc ggtggcgacc gcctctgtgc tggtagcctc
     3061 accgtcacgc cccatcgtgc ctgcaacgca tccggtgacg gtccgtcctc cggtgtcggt
     3121 ctggcccgaa cctgaggtgg tcgccgcgac gcgtgaggcc ggcgggg
//
LOCUS       DAORHD010000492         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_34844_length_3166_cov_3.439087, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000492 DAORHD010000000
VERSION     DAORHD010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34844_length_3166_cov_3.439087"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKO06_10965"
     CDS             complement(<1..443)
                     /locus_tag="PKO06_10965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV70210.1"
                     /translation="MTSPIVRAFAQWVEGQPTKPALHFDSQTLTYGELASKVGRLSNG
                     LYQTGMRPGDHVGVMLPNGAEFVLFMLAAADLGLVIVPQNLTMSGSGLAGSFNAAHVT
                     HVIGWHSVIDPFKGSNEGFRISPRTTWITLGGAVDGCLRFEDLLT"
     gene            complement(507..2438)
                     /locus_tag="PKO06_10970"
     CDS             complement(507..2438)
                     /locus_tag="PKO06_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV70211.1"
                     /translation="MKQDYYSRNMKVLRKRQPLLAKALEMEFPALGEYLVEPAKNGSP
                     TLAIKTPDGKLHQVHSRYDPEKEARQQILGQKYRNPKLFVALGLGLGYHIRAVLEELK
                     EDVYFIVVIERDMHALRAAITHADISDLLESEKIRWAVGIPENEGFAVLNELIKAASI
                     TFQLFLKTMVVFEHPILARVCGDYYRLMVRHFREAAHAVIFNYGNCPNDSLIGVRHIM
                     RNLSIIMRNPGISDLYNVFKGKPGIIVSSGPSLDGNMHLLKGIEDHCVMVAADSALKV
                     LLKNGIRPHLVSSLERHHLVAELFRDIPREDLDGIWLAGTPVLMPNVYEAYAGDTIIV
                     YRAFAHFEWLQMPKGTLNIGPSCSNMAFKTLEALGCNPIILVGQDLSFRDEKKTHVEG
                     IPEVTNLKLKKENLFPIKGNFQETVLTNAIFDMFRKAFVTDIAQFRGTCINATEGGAY
                     IEGAKLMPLREVIERHLQVPLDTVATIRKHLHLPTEHEVKEEWHRFRKTMIETRKEVQ
                     AVIEFCDKGVQMVVDFENTLNTEGFQEIEDFLKRFPEDQLDKIFTELTQARGKVITFG
                     KYFNLYLMHIVQMIIVKFEMDFNELGSLCDDKKRIKLQAIRMMRQWFPTIGDLCRISL
                     QLLEEAFDKLEKEFGNPDA"
     gene            complement(2522..>3166)
                     /locus_tag="PKO06_10975"
     CDS             complement(2522..>3166)
                     /locus_tag="PKO06_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70212.1"
                     /translation="SLSTAQAAIHTVIGQIESVYMNRASMTILDTRTASDTPETLSVG
                     KFVSFNLPAQGGKKKTSVQYGNIVEVDLAGDIATEYGGQVASDATKVFIWTAVRCDKV
                     KNAKKYTSGKDGEKKGKGKGKGKKKDEIPDRLWTQEEAVRGTISYREKDKKLYIREQG
                     LRPRDKGLDVVTDEWFEKLKPFNGQQVVVHGTTHRTSLASGTIEIDNILRVYPK"
BASE COUNT          655 a          827 c          903 g          781 t
ORIGIN      
        1 cttgtcagaa ggtcttcaaa acgcaggcat ccgtcaacgg ctcctcccag cgtgatccag
       61 gtggttcgcg gcgagatgcg gaacccctca ttggatccct tgaacggatc aatcaccgag
      121 tgccagccga tgacatgggt gacatgagcg gcgttgaacg aacctgccag accagagccg
      181 ctcatggtga gattctgcgg tacgatgacc agtccgagat cagctgcggc cagcatgaag
      241 agtacgaact cagcgccgtt gggcagcatg actcccacgt ggtcaccggg gcgcattccg
      301 gtctggtaaa gaccgttgga aagccgcccg actttgctgg ccaactcgcc atacgtgaga
      361 gtctgcgagt cgaaatgcaa ggctggtttt gtgggctggc cttcaaccca ctgagcgaag
      421 gcgcggacga ttggggaggt catcgttctt cactccgaag aaacacgaga ttcgatgaga
      481 atgtcggcag atcaaaactc ggacaatcac gcatcggggt tgccgaattc cttttccagt
      541 ttgtcaaagg cctcttccag gagttgcagt gaaatgcggc agaggtcgcc gatggtcggg
      601 aaccactgtc gcatcatacg aatggcctgt agcttgatgc gcttcttgtc atcacacaga
      661 gaaccgagtt cgttgaaatc catctcgaat ttgacgatta tcatctgcac gatgtgcatg
      721 aggtacaagt tgaagtattt gccgaaggtg atgaccttgc cgcgggcctg agtgagttcg
      781 gtaaagattt tgtcgagctg gtcctcggga aatcgcttca ggaagtcttc gatttcctga
      841 aatccttcgg tgttgagggt gttctcgaaa tcgacaacca tctgcactcc cttgtcgcag
      901 aattcgatga ctgcctgcac ctctttgcgg gtctcgatca tggtttttct gaatcggtgc
      961 cactcctctt tgacctcgtg ttctgtgggg aggtgcagat gcttgcgaat tgtggcaacc
     1021 gtgtcgagcg gcacttgcag gtgccgctcg atcacctctc gaaggggcat gagtttcgcc
     1081 ccttcgatat aggctccgcc ttcggtggca ttgatgcagg tcccgcggaa ctgagcgata
     1141 tcagtcacga aggctttgcg gaacatgtca aagatggcat tggtgagaac tgtctcctga
     1201 aaattccctt tgatgggaaa caggttttcc tttttcaact tcagattcgt aacttcgggt
     1261 attccctcga catgcgtttt cttctcgtcg cggaacgaga gatcttgacc gacaaggatg
     1321 atcgggttgc acccgagcgc ttcgagtgtt ttgaaggcca tgttggaaca ggacgggccg
     1381 atgttcaggg ttcctttggg catctgcagc cattcaaaat gcgcgaatgc gcgatagacg
     1441 atgatggtgt caccggcata ggcttcatac acattgggca tcaggacagg ggttccggcg
     1501 agccagatcc cgtcgagatc ctctcgcgga atgtcgcgaa acaactcggc gaccaggtga
     1561 tgccgttcga gcgaagagac gagatgtgga cggataccgt tcttcagaag aactttcagg
     1621 gctgaatcag ccgcgaccat gacgcaatgg tcttcgatgc ccttcaagag atgcatgttt
     1681 ccgtcgagtg acggaccgct tgaaacaatg atgcctggct ttcctttgaa tacattgtac
     1741 aagtcgctga tgcctggatt gcgcatgatg atgctgagat tgcgcatgat gtgtctgacc
     1801 ccgatcagcg aatcgttggg gcagttgccg tagttgaaga tgacggcgtg cgcggcttcg
     1861 cgaaagtgcc tgaccatcag acgataatag tcgccgcaga cgcgcgccag gatgggatgt
     1921 tcgaagacga ccatcgtttt caggaacagt tggaaggtga tgctggcggc cttgatcagt
     1981 tcattcagaa cggcgaaccc ttcgttttcc ggaataccga cggcccagcg gattttctct
     2041 gactcgagca ggtcgctgat gtcggcgtga gtgatggccg cgcgcagagc gtgcatatcg
     2101 cgctcgatga ccacgatgaa atacacatct tccttgagtt cctcgaggac ggcgcggatg
     2161 tgatatccga gccccagacc cagggcgacg aagagtttcg ggttgcgata cttctgtcca
     2221 agaatctgtt gacgtgcctc tttttcagga tcataccgac tgtgcacctg atgcagcttg
     2281 ccatccggag tcttgatcgc cagagtgggg gagccgtttt tcgccggttc caccagatac
     2341 tctccgagtg cgggaaactc catctcgagc gcttttgcaa gcagaggttg ccgcttgcgc
     2401 aacactttca tgttgcgact gtagtagtcc tgtttcatga acgatggtct ccgatactca
     2461 ttctatgcca atctgagcca gatgagactc ggacggacat gcgggctgac accacggtgc
     2521 gttacttcgg atacacgcgg agaatgttgt cgatctcgat ggtgcctgag gccagtgagg
     2581 tccggtgcgt ggttccgtga acgacgacct gttgcccgtt gaacggtttg agtttttcaa
     2641 accactcgtc cgtcacgacg tcgagaccct tgtcgcgcgg tcgcagcccc tgttcgcgga
     2701 tgtagagctt cttgtctttc tcacgataac tgatggtacc gcgaacggcc tcttcctggg
     2761 tccagagacg atcgggtatc tcgtccttct tttttccctt ccccttgccc ttgcctttct
     2821 tttctccatc cttgccggaa gtgtactttt tggcgttctt gaccttgtcg cagcgtacgg
     2881 cagtccagat gaacactttg gtggcgtcag acgcgacctg cccaccatac tcggtggcga
     2941 tgtcaccggc cagatcaact tcgacgatgt ttccatactg gacagacgtc ttctttttgc
     3001 cgccctgtgc cggcaggttg aaactgacaa atttgccgac cgagagggtt tccggagtat
     3061 cgctggcggt cctggtgtcg agaatcgtca tcgaggcgcg attcatatac acggactcga
     3121 tctgcccgat gaccgtgtga atggctgcct gagctgtgga caaaga
//
LOCUS       DAORHD010000493         3152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35072_length_3152_cov_3.156603, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000493 DAORHD010000000
VERSION     DAORHD010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35072_length_3152_cov_3.156603"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PKO06_10980"
     CDS             <1..476
                     /locus_tag="PKO06_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70213.1"
                     /translation="SLGADVNLAFTKTIQLKGEIWQGKNLDDYVGGIGQGVVVTTTGS
                     GTYINAMAAGATGFVDAKALKSKGGWAELTFGPFQKWRYNLGVSLDNPDDDLLINSTT
                     ARPMRTSNRSRWLNALYDLNEAVQIGAEYLRLETEYKNQRRGDDNRFQLSFIYKF"
     gene            complement(596..1912)
                     /locus_tag="PKO06_10985"
     CDS             complement(596..1912)
                     /locus_tag="PKO06_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV70214.1"
                     /translation="MTIVDALIFGLLLCGSAFFSGTETALTSVQESKLSTLAEKGNRA
                     AQRVMALIANRGTVIGAMLIGNNIVNTLLAIYAATVFNAVILNSPLPPWMAPVAASVL
                     SIVVLLIGGEVVPKNIGLRFNTRWALLAAYPCTYFIKLVTPVLWGLNLINRLMLSILG
                     GQDTRSGPSVDELLALVKMSQKAGIIDPMERELIGKSMFLNETLAKEIMIHRTQVCAV
                     AETSTLADVQAVFVRELFSRLPVYRENLDQIVGILNIKEVFRFDAKNTGFRIADVMTP
                     PLVYPETAKIGAIFDKMRQSRSHMVIVVDEFGSTSGIITLEDIVEQIFGEISDEYDAQ
                     SPARFRWVGAQVFEAEGRTSLSEVQEELEKKHCRVLPADACEDVETLAGLVMKVLGNI
                     PSAGERFEVGGFHFQIRKVTGQKIQLISVRVPICEGGKSDETPVAV"
     gene            complement(2108..2437)
                     /locus_tag="PKO06_10990"
     CDS             complement(2108..2437)
                     /locus_tag="PKO06_10990"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV70215.1"
                     /translation="MNKVYKKFIASFKIQLKRRYMMLFKKEEVAETLKLRRGECLSCG
                     ECCRSSFPCPFLFERDGLELCRIHADKPEPCRLYPFSPEDVFPHTEGKCGYYFITPEE
                     AEKESKS"
     gene            complement(2538..>3152)
                     /locus_tag="PKO06_10995"
     CDS             complement(2538..>3152)
                     /locus_tag="PKO06_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV70216.1"
                     /translation="LKREGVSIPFPIREVFTHPEKVEQAQMMEHRLELISQIDFLRDL
                     ERPLRALLATCLVEFWFEKSEVIVQKGSTGTDFYLVDKGSVDIFIDFPGGEPVARLAG
                     GQFFGEMSLLTGEPRSATVVAAEETRVLVLSKETMGKVLRDHDELAKILGEALAQRQM
                     ANQSIQMPRGNESGIESAEQRYAADLARSRSHFMTRIRKFFGME"
BASE COUNT          735 a          967 c          796 g          654 t
ORIGIN      
        1 cctcgcttgg cgcggacgtc aacctggcgt tcaccaagac gattcaactc aaaggtgaaa
       61 tctggcaagg caagaacctc gacgattacg tcggtggcat cggtcaggga gtggtcgtga
      121 ccaccaccgg ttcaggcacc tacatcaatg ccatggcggc aggcgcgacc gggttcgttg
      181 acgccaaagc tctcaagagc aagggcggtt gggcggaatt gacattcggc ccctttcaga
      241 aatggcgcta caacctcggt gtaagcctcg acaacccgga cgatgatctg ctcatcaact
      301 cgaccacggc gcgacccatg cgcaccagta accgctcacg ctggctgaat gcactctacg
      361 acctgaatga agcggtacaa atcggcgccg aatacctgcg ccttgaaacc gagtataaga
      421 accagcggcg tggcgacgac aatcgtttcc agctctcgtt catttacaag ttctagaacc
      481 agcagccaag gctgcgaaca gaacgccccc gccggagtac cggcgggggc gttctgtttc
      541 gcctgctgtc ttctgatcgc gatcaagtca gcagcaggtc agtggcagtg atcactcaca
      601 cggcaacggg tgtctcgtcg ctcttcccgc cttcgcaaat cggcacacgc actgaaatca
      661 gctgaatctt ctgccccgtg actttccgaa tctgaaaatg aaagccaccc acctcgaatc
      721 gctcgccggc agacggaatg tttcccagaa ccttcatcac cagaccagcg agagtctcta
      781 cgtcttcaca ggcatcagcc ggcagaaccc gacagtgctt tttctcgagt tcttcctgca
      841 cctctgagag cgaggtccgg ccttcagcct cgaacacctg cgcacccacc cagcgaaaac
      901 gcgccggact ctgggcgtcg tactcgtcgc tgatctcgcc gaagatctgt tccacaatat
      961 cttcgagagt aatgatgcca gaagtcgagc caaactcgtc gacgacaatc accatatgtg
     1021 agcggctctg cctcatcttg tcgaaaatgg caccgatttt cgcggtctcg gggtagacca
     1081 agggtggggt catgacatcg gcgatgcgaa acccggtgtt tttggcatca aaccggaaca
     1141 cttccttgat gttgagaata cccacgatct gatcgaggtt ctcacgatag accggcaggc
     1201 ggctgaacaa ctcgcgcaca aataccgcct gcacatccgc caatgttgaa gtttctgcaa
     1261 cggcgcagac ctgagtgcgg tgaatcatga tctctttcgc caacgtctcg ttcagaaaca
     1321 tcgacttacc gatcaattca cgctccatgg ggtcgatgat acccgctttc tgactcatct
     1381 tgaccaaggc cagaagctca tcgaccgacg gccccgaacg cgtatcctgc ccgcccagaa
     1441 tcgacagcat caaacgattg atcaggttca aaccccagag aaccggggtc accagtttga
     1501 tgaagtaggt gcacgggtac gccgccagca gcgcccatcg agtgttgaaa cgcagaccga
     1561 tgtttttcgg aacaacctct ccaccgatca gaagcaccac gatcgaaaga accgacgccg
     1621 cgaccggtgc catccagggc ggcagcggag agttgaggat cacggcattg aacacggtcg
     1681 cggcgtagat tgccagcagg gtgttcacga tgttgttgcc tatcagcatc gccccaatga
     1741 ccgtgcctcg gttggcgatc aaagccatga cccgctgtgc cgcgcggttt cccttctctg
     1801 ccagcgtcga cagcttcgat tcctgcaccg acgtgagagc ggtctccgtc ccggaaaaaa
     1861 acgctgaccc acacagaagc aggccgaaaa tcagcgcatc aacaatggtc attccaactc
     1921 ccgtccttca agaaccgagc tctctcactc tggcacggtc acatcttgag ccaaaagcag
     1981 caatcaactt ctcttcgctc tcctgatgcc atcgcataca actgcgcctg tcgtgctgaa
     2041 agtcgcaact tttccggaat tcaggagacg ttccggcagg ggccacacgc tggtgagcag
     2101 cgttgagtca gctcttcgac tctttctccg cttcttccgg ggtgatgaaa tagtacccgc
     2161 atttgccttc ggtgtgcgga aaaacgtctt cagggctgaa agggtacagg cggcagggct
     2221 ccggcttatc cgcatgaatg cggcacaact cgagaccatc ccgctcgaag aggaatggac
     2281 acgggaacga cgaccggcaa cactcgccgc aactcaaaca ctcgcctcgt cgcagcttca
     2341 gcgtttccgc cacttcctct tttttgaaga gcatcatgta gcgccgcttg agctgaatct
     2401 tgaacgatgc gatgaatttt ttatagacct tgttcacccg ggcctcccgg aaggggaaaa
     2461 tcaaaccctt gcccgttcgg acaatggcaa agaattatat cagcgcttct attccacggt
     2521 caaattctcg ccaaccctca ttccatgccg aaaaacttgc gaatccgcgt catgaagtgg
     2581 ctgcgagagc gggcaagatc agccgcatat ctctgttctg cgctctcaat gccactttcg
     2641 ttccctcggg gcatctgtat ggattgattg gccatctgac gttgagccag agcttcgccg
     2701 agaatcttcg ccaattcatc gtgatcacgc aacacctttc ccatggtttc cttcgacaaa
     2761 accaatactc gcgtttcttc tgccgcaaca accgttgccg aacgcggctc accggtcaac
     2821 agcgacatct cgccgaaaaa ctggccgcct gccagacgag cgacaggctc gccaccggga
     2881 aaatcgatga aaatatcgac actgcccttg tcgaccaggt agaagtcggt tccggttgag
     2941 cctttttgca cgatgacctc gctcttttcg aaccagaatt cgaccagaca cgtggcgaga
     3001 agcgcccgca gcgggcgttc gagatctcgc agaaaatcga tctgcgaaat cagctccaga
     3061 cgatgttcca tcatctgcgc ctgttcgacc ttttccggat gcgtgaagac ctcgcgaatc
     3121 gggaacggga tactgacccc ctcgcgtttg ag
//
LOCUS       DAORHD010000494         3151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35090_length_3151_cov_3.158592, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000494 DAORHD010000000
VERSION     DAORHD010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35090_length_3151_cov_3.158592"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..1360
                     /locus_tag="PKO06_11000"
     CDS             185..1360
                     /locus_tag="PKO06_11000"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70217.1"
                     /translation="MSILPRYLTRMVRADALVAGKMAFLSGPRQVGKTTIGRALLEHE
                     ENAFTWDDDDFRRRWVKSPAEAIAARESGPVLFDEIHKDVRWKQKLKGLYDTVREKLP
                     IIVTGSARLDVFRRGGDSLLGRYLPYHVHPFSVGETENPPGPDEIMTRTKVSFPLTDV
                     LKLGLFPEPLLGGSEARARRWSRLRRERLITEDVRDLRNVQSLRQLALLVDLLPSRVG
                     SLLSINSLREDLGTAHDTVSAWIETLSSVYLCFRISPWSKRIARAVRAEPKLYLFDFL
                     QVPEGGPRLENLIALHLLKACHFWTDSAQGEFTLHFIRDRDGNEVDFLVTREQKPWML
                     VEVKSGETSPSKTLMKYAAVLETRLNFQVVSKPDVRRRYPDSDITVISAATFLAGLV"
     gene            complement(1771..2277)
                     /locus_tag="PKO06_11005"
     CDS             complement(1771..2277)
                     /locus_tag="PKO06_11005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNV70218.1"
                     /translation="MRASAGHSHEGPVHDQSTQRLRVHKLDKWLTYLSNVQGKALETI
                     AMSTPAIKKALTIEEIFAQSDHERRLYELREKGLHDLATIRATGREEGRAEGREEGRE
                     EGREQRNREIARRLLSKGYTLEQVAEMVGLTIDETRALSERKVQELPARFGTARPVGT
                     KTRRRKPA"
     gene            2281..2526
                     /locus_tag="PKO06_11010"
     CDS             2281..2526
                     /locus_tag="PKO06_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70219.1"
                     /translation="MTIIPSEPEHQQRDCWFVTRTCLKILATLKELSASLALRGVTVS
                     SADLWIGSTALVHGLTLVTHKLKDFRKNPGLDLETWP"
     gene            complement(2551..>3151)
                     /locus_tag="PKO06_11015"
     CDS             complement(2551..>3151)
                     /locus_tag="PKO06_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70220.1"
                     /translation="VVYAWGDGSYGQTGNRIASAAFNPRVALVVNSGGTRISNIRQVG
                     CTLNTSFAVSSAATVLAWGGSENGELGNGTTVKSRAFSANVRRSSARPGANLNTALRI
                     ACGDRHILVLTTSNTVVGWGQNTSQQINTAGGTLLYPATVSGLTSVEALSAGSKHSLA
                     LRNSSTDLYTWGTDAYGVLGNGLPLDDVTSPTTISYPAD"
BASE COUNT          624 a          928 c          903 g          696 t
ORIGIN      
        1 cccgcgacca cgtagttctc ccccacgtcc cttggcaccc ccggcgagag tgagtcactg
       61 agcgactcca gagggtatcc tgcacggagt gcttgctttt cccgcattgg cagactacta
      121 ttgaactgga agaatgccgc ggcaatattc cagtgagctg gaagtctgcc gaactggggg
      181 ccgtatgtcg atcctgccaa gatacctgac ccggatggtg agagccgatg cgttggtcgc
      241 gggcaagatg gcctttcttt ccggacctcg acaagttggg aagacgacca tcgggcgcgc
      301 ccttcttgaa cacgaggaaa atgccttcac ctgggacgac gatgactttc gacggcggtg
      361 ggtgaagagt ccggctgaag ccatcgccgc ccgcgagtcc gggccggtgc tgttcgatga
      421 gattcacaaa gacgtccgct ggaagcagaa actcaaagga ctgtatgaca cggtccgcga
      481 aaaactgccg atcattgtca ccggaagcgc tcggctggat gtctttcgcc ggggtggtga
      541 cagtttgctt ggtcgatatc tgccctatca tgttcacccg ttttcggtcg gggaaacaga
      601 gaacccgcct gggccagacg agataatgac tcgtacgaaa gtgtcgtttc cactgacaga
      661 cgttctgaaa cttggtcttt ttcccgagcc gctcctcggg ggtagtgaag cgcgagcccg
      721 ccgctggagc cgtctgcgtc gtgagcgtct catcaccgaa gatgtgcggg atctcagaaa
      781 tgtccagagt cttcgacaac tggcactgct tgtcgatctt ttgccctcgc gcgttgggtc
      841 gctgctgtcg atcaacagct tgcgcgagga tctcggtacc gctcatgaca cggtgagtgc
      901 ctggatcgaa accctgtcca gcgtgtatct ctgcttccgg atcagcccct ggtcgaagag
      961 gatagcgcgg gcggtccggg cggaaccgaa actctatctg ttcgacttct tacaggttcc
     1021 ggaaggcggc ccacggctcg agaacctgat cgctctgcac ttgctcaaag cctgccattt
     1081 ctggaccgac tcggcgcagg gggagtttac tctgcatttc atcagagacc gtgacggcaa
     1141 cgaagtcgat ttcctggtga cacgcgaaca gaagccctgg atgctggttg aggtgaaaag
     1201 cggtgagacc agtccatcca aaacgctgat gaaatacgcc gctgtgcttg aaaccaggct
     1261 gaatttccag gtggtctcaa agcctgacgt ccggcgaagg tacccggact ctgacatcac
     1321 cgtcatttcc gcggcaacat tcctcgccgg tctggtctga accagcctgt cgatcgaaac
     1381 cgagacaacg actgcctggc gtggtgattt ttttgtcgct ccgatgacga cggcgggctc
     1441 agactatccc acacgcattc cggtggtttt cgacggcagg aagggccagc gtgcagacaa
     1501 aggcgaacga ttcgtgctgt tgaaggggaa gaaagcggtc gcggaatcgg gttgatcttg
     1561 ccgcactgat cggcgcgcgc cccgccgtga gcttttttat ctccgtcatc acggtcagtg
     1621 atgttcccgt ttcccgatcc ttgtcgcttt gctccattca tctggtcgaa tcaatcagat
     1681 tgaccggtcg aattttcaga atacgcagtc atcgaaacga aacagcccgc gccgacccac
     1741 acccgcgatg acggggtggc ggaaaagcag ttatgccggt ttgcggcggc gggtcttcgt
     1801 gcccacgggc ctggcagtgc cgaatctcgc cggaagttct tggactttcc gttccgacaa
     1861 agcccttgtt tcgtcgatcg ttaaacccac catctcagca acctgttcaa gcgtataacc
     1921 tttcgacaag agacgtcgcg cgatctcacg atttcgttgt tcgcgaccct cttcacggcc
     1981 ttcttcgcga ccctcggcac ggccctcttc gcggccggtg gcgcgaatcg tggccaggtc
     2041 gtgcaggccc ttctcgcgca gttcatacag gcggcgttcg tggtcgctct gcgcaaaaat
     2101 ttcctcgatg gtcagagctt tcttgatcgc gggtgtgctc atggcgatgg tctccagtgc
     2161 ttttccctga acgttgctca gataggtgag ccatttgtcc agtttgtgaa cgcgtagtcg
     2221 ttgagtcgat tgatcatgaa ctggcccctc gtgtgagtgt cctgcgctcg ctcgcataac
     2281 atgaccatca taccatccga accagaacat caacaacgtg actgctggtt cgtcacccgc
     2341 acctgcttga agatcctcgc taccctgaaa gaactttccg cctcactcgc cctgcgtgga
     2401 gtcaccgtca gttccgccga tctctggatc ggcagtaccg ccctcgtgca tgggctgacg
     2461 ctggtcaccc acaaactgaa agacttccgc aagaatcccg gcctcgacct cgaaacctgg
     2521 ccgtgatgac tcgcacgccg ggtccgagct tcagtccgcg gggtaagaga tggttgtagg
     2581 cgacgtgacg tcgtccagcg ggagtccatt tcccaaaact ccgtaggcat cggtacccca
     2641 agtgtacaag tccgtcgatg agtttcgcag tgctagagag tgtttcgatc cggcgctcag
     2701 ggcctcaacg ctcgtcagcc cggaaaccgt tgctgggtag agcagagtgc cgccggctgt
     2761 gttgatctgt tgggaggtgt tctgacccca cccgaccacc gtgtttgagg tggtcagcac
     2821 gagaatgtga cgatcaccgc aggcgatccg aagtgcggtg ttaaggtttg cccccggccg
     2881 tgcagaactg cgcctgacat ttgcggagaa tgctcgtgat ttaaccgttg ttccgttccc
     2941 gagttccccg ttttcgctac ccccccaggc gaggacagtc gcggcggaac tgacagcgaa
     3001 cgaggtgttc aaagtacacc cgacctgccg gatattcgag atgcgtgtac caccggaatt
     3061 gaccaccaga gcgacacgcg gattgaaggc ggcactcgcg atcctgttcc cagtctgccc
     3121 gtacgagcca tcgccccagg catagacaac t
//
LOCUS       DAORHD010000495         3150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35109_length_3150_cov_2.956381, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000495 DAORHD010000000
VERSION     DAORHD010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35109_length_3150_cov_2.956381"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKO06_11020"
     CDS             <1..352
                     /locus_tag="PKO06_11020"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNV70221.1"
                     /translation="LPGMLVLSCVLTVLMSICLFWSISGEGVTRMLPNLAYLFSGMVI
                     PLPFFPEALQSYLFFTPFAGLVDHPYRLYTGHLAPQSAFAVVATQWFWIFVLVAVGRL
                     MLRHGVRRLVIQGG"
     gene            360..1073
                     /locus_tag="PKO06_11025"
     CDS             360..1073
                     /locus_tag="PKO06_11025"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HNV70222.1"
                     /translation="MWETMRLYSRYLAISLRAQTEYRLSFLLIALGQLAATGIEFIGI
                     WALFRRFGRLNGWELHEAAVLYGVVHVAFALAESIFRGFDQFAPLLKSGEFDRLLLRP
                     RSTAFQVLARELELGRVGRLLQGALVLFWGLSCLPGGVTLVTGVVTISAIVGGIALFG
                     GLFVLQATMAFWTIESLEIWNTLTYGGVETAQYPLSIYHPWFRRLFTFLIPLACINYF
                     PVQIILGRQTLSDITRRMV"
     gene            complement(1402..2634)
                     /locus_tag="PKO06_11030"
     CDS             complement(1402..2634)
                     /locus_tag="PKO06_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70223.1"
                     /translation="MEFSTVKRFVLVVFVLVAACLYAAYDGSKPADNEFVADVPALVR
                     ENFRAVKDDAIVNAGAVSGYSVGSASGNIPRNNGTLNTNLNSDKLDGYHASDFTASSS
                     YSASDVLTKIKTVDGAGSGLDADLLDGYTASTTVNAPNTLILRDASGYAQLGWINTTS
                     GDNGTTTPTRIYASNDGYIRYYSLANFVTVTSMVTLTGTQTLTNKTLSTGCTWNGNAI
                     ADGYITAAVARDTELSAHAAVTATHGATGAVVGTTNTQTLTNKTLSTGCTWNGNAIAD
                     GYITAAVARDTELSAHAAVTATHGATGAVVGTTNAQTLSNKTIGNTCSIIALHDSGWI
                     SVGAASSYTYSGNLGYGSSVFYIIWHADGNPGQSYFLGGSLDPYISAGYNSSTGKYYF
                     YNGHGAARYFRIVFYNFD"
     gene            complement(2635..>3150)
                     /locus_tag="PKO06_11035"
     CDS             complement(2635..>3150)
                     /locus_tag="PKO06_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70224.1"
                     /translation="QAGKTWGKYACVQKDVGSIITARIAVDWFVMALAATGMAGVLRF
                     RSSLNGILWTDWAVFEPVSMTFRYLEFEIELTSTNPTKTPEVTLCTITVDVPDKEINF
                     ADHTILAAGTEIQYGHTFHEIPSVSPTALGGNKAAELISKDETKCFIKIREVSTGNYV
                     AGTADIRVRGY"
BASE COUNT          608 a          834 c          885 g          823 t
ORIGIN      
        1 tctgcccggc atgctggtgc tcagttgcgt tctgacggtg ttgatgagca tctgtctgtt
       61 ctggagcatt tctggtgagg gggtaacccg catgctgccg aacctggcct acctcttttc
      121 cggcatggtc attcccctgc cgttttttcc cgaggcgtta cagtcgtatc tgttcttcac
      181 cccattcgcc ggtctggtcg atcatccgta ccgcttgtat acaggacatc ttgcgcctca
      241 gagcgcattt gccgtggttg ccacccaatg gttctggatt tttgtcctgg ttgccgttgg
      301 ccgtttgatg cttcgccacg gcgtgcgtcg cctggtaatt caaggagggt gagcgaccca
      361 tgtgggaaac catgcgtctg tattcccgat atctggcaat ctcgctccgg gcgcagaccg
      421 agtatcgcct gtcctttctg ctgatcgcgc ttggacaact ggcggcgacc ggtattgagt
      481 tcattggcat ctgggccctg tttcgtcgtt tcgggcgcct gaatggctgg gaactgcacg
      541 aagcggccgt tctctacggc gtcgtccatg tcgctttcgc gcttgccgag tcgatctttc
      601 gcggttttga tcagttcgcg ccgttactga aaagtggcga gttcgaccgt ctgctgctgc
      661 gcccgcgctc gacggcgttt caggtcttgg cccgtgaact cgaactgggg cgggtggggc
      721 gtctgctgca gggagccctg gttctgttct ggggcctgtc atgcctgccg ggaggcgtga
      781 ctctagtgac cggtgtcgtg acgatctcgg cgatcgtggg tggcattgcc ttgtttggcg
      841 gcttgtttgt gttgcaggca accatggctt tctggaccat tgaatcgctg gaaatctgga
      901 acaccctgac ctacggtggc gttgaaaccg cgcaataccc gctgtcgatt tatcacccct
      961 ggtttcgtcg gctgttcacg tttctcattc cgctcgcctg catcaactac ttcccggtgc
     1021 agattattct cgggcgacag acgctgtctg atataacccg tcggatggtt tgacagaatt
     1081 ttgcacatga aatgatacat ggtttcggaa aaaagagcct ggtttttgcc aggctcttcg
     1141 attttatcct gcttcgacct ttatgcagtg aaattgcaga tcagaagctc gcgagcctcc
     1201 tttccccgat ctttggcctg tagcatctcg agttaattga ccgggaatcg ggcatagttt
     1261 ctcatgctcg cttaccggtg gtgagaatgt tccggtaacc gagtgtgaga acagttcggt
     1321 attggagcgt gagacggatc gtttcaaaca ctctttcaca gcctgtcacc ctgaccacaa
     1381 gcctttgcaa ccaggacatg tttaatcgaa gttgtaaaac acaatccgga agtatctggc
     1441 ggcgccgtgt ccgttataaa agtagtattt tccggtgcta gaattgtatc cagccgaaat
     1501 ataggggtcc agactccctc cgagaaagta tgactgacct ggattgccgt cagcatgcca
     1561 aataatgtag aagacactcg atccataacc gaggttgccg gaatacgtgt aagatgacgc
     1621 cgcccccacc gatatccacc cggaatcgtg cagagcgata atggagcatg tgttccctat
     1681 tgttttgttc gacagtgtct gtgcgttcgt cgttccaacc accgctccgg ttgcgccgtg
     1741 tgttgcggtc acggcggcgt gggccgataa ttcggtgtca cgggcaacgg ctgctgtgat
     1801 gtaaccgtcg gcgattgcgt tgccattcca agtgcagccg gtgctcaggg tcttattggt
     1861 cagagtctgc gtgttggttg ttcctacaac agctccggtt gcgccgtgtg ttgcggtcac
     1921 ggcggcgtgg gccgataatt cggtgtcacg ggcaacggct gctgtgatgt aaccgtcggc
     1981 gattgcgttg ccattccaag tgcagccggt agagagcgtt ttgttcgtga gggtctgtgt
     2041 gccggtcagg gtaaccatcg atgttaccgt cacgaagttg gcaaggctgt aatatctgat
     2101 gtagccgtcg ttagaggcat agatgcgagt cggagttgtg gttccgttgt caccggacgt
     2161 ggtgttgatc cagcccaact gggcgtagcc gcttgcatct ctgagtatta gagtgttcgg
     2221 tgcattgacc gtcgtgcttg ctgtataacc atccagcaga tcagcatcga gtccgctccc
     2281 cgcgccgtcc acggtcttga tttttgtaag cacgtcagat gctgagtaag acgacgatgc
     2341 cgtaaaatca gaagcgtgat acccgtccaa cttgtctgag ttcaaattcg tgttgagggt
     2401 cccgttattc cgtggaatat tgccggatgc ggaccctact gagtagccgg atacggcacc
     2461 ggcgttcaca atcgcgtcat ctttgacggc tcggaagttc tcgcgaacca atgcgggaac
     2521 atcagccacg aactcattgt cagcaggctt tgagccgtca taagcggcat acaggcacgc
     2581 ggcgaccagg acaaacacca caagaacaaa gcgtttcaca gttgagaatt ccattcaata
     2641 ccccctgact cgaatatctg cagtcccagc cacataattg ccggtactga cttcacggat
     2701 tttgatgaag cacttggtct cgtctttcga gatcaattcg gcagccttgt ttccacccag
     2761 tgctgtcggt gacacagacg ggatttcatg aaacgtgtgc ccgtactgaa tctccgttcc
     2821 ggccgcgaga atcgtatgat cggcgaaatt gatttcctta tcgggcacat cgacggtgat
     2881 ggtgcacaac gtgacttccg gggttttcgt cgggttcgtg ctcgtgagtt cgatctcgaa
     2941 ttcgagatag cgaaaggtca tagatacagg ctcgaatacg gcccaatcag tccagagaat
     3001 gccgttcaaa gaagaacgaa aacgcagaac tccggccatc ccggtcgcgg cgagagccat
     3061 gacgaaccag tcaaccgcga ttcgcgcggt gatgatgctg cccacatcct tttgcacgca
     3121 ggcatacttt ccccaggtct ttccggcttg
//
LOCUS       DAORHD010000496         3146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35177_length_3146_cov_3.560660, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000496 DAORHD010000000
VERSION     DAORHD010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35177_length_3146_cov_3.560660"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..118)
                     /locus_tag="PKO06_11040"
                     /pseudo
     CDS             complement(1..118)
                     /locus_tag="PKO06_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002759436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-beta hydroxysteroid dehydrogenase"
     gene            198..1007
                     /locus_tag="PKO06_11045"
     CDS             198..1007
                     /locus_tag="PKO06_11045"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV70225.1"
                     /translation="MLARARSFFGCYEVVASEQRTLRYLNHGNIVHGGQCVSSILRDV
                     PILYYHPSGPTGQVLQQYNRQRNVRVGIVGMGAGMVAAYSRFSHEYVFFEIDPVVIRI
                     ARDPHLFTYLADARGKIRVVCGDGRLGLAAEPEHSFDVLMFDAYSSDAIPVHLLTREA
                     MQLFLSRLKPGGIMLFHLSNRYLRLEPVLGNLCEDLELTAVIQDHEPDEKKLPPSEVG
                     LVSSSRWALVASESANLVKWLPDPRWKLLSGDPRVGVWTDQFSSILSVFRK"
     gene            complement(1423..1830)
                     /locus_tag="PKO06_11050"
     CDS             complement(1423..1830)
                     /locus_tag="PKO06_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV70226.1"
                     /translation="MEVKPAPHDQSSAVEIVREFWRLMSTNDFVSVGTVLHEDFVLEW
                     PQSNERIRGAQRFAQMNSQYPAHGTWTFTINRLFGSDLEAASDVSVTDGVQTARAISF
                     FTIRDRKIARLVEFWPEPYPAPANRAHLVEPIT"
     gene            complement(1990..2835)
                     /locus_tag="PKO06_11055"
     CDS             complement(1990..2835)
                     /locus_tag="PKO06_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV70227.1"
                     /translation="MKRFSLILVLFALFACCLPTFADGDENVKKFHEAIIKGDLPTVQ
                     AMIDGGQMNANSKLIGEKDRPLMLAIISTKPDIVKYLIDKGADVNFVANQGLTPIMNA
                     VGHMPIDIVKLLLEKGAKIDVANDDKMTPLHWAAYRGNSEAIALFLEKGANLKAISEN
                     VMGLGGTPLHSAALGNRADAVKLLVEKGASIDEQNQKGYTPLMLAARNGGLKTIETLL
                     SLKANVNLANADKKKAHQIAKERLAEKLESAKKNPKYDEANPPQEIKDFQAVIDLLIK
                     AGAAD"
     gene            complement(2900..>3146)
                     /locus_tag="PKO06_11060"
     CDS             complement(2900..>3146)
                     /locus_tag="PKO06_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70228.1"
                     /translation="SQRPDNPFIHRMYGSEVRYAYKFVKKKAGSYTPPLRKKIYDESL
                     PRFGVSSGVNGNSSDITQFVIVSWQDLRAGDDQYNSF"
BASE COUNT          692 a          795 c          878 g          781 t
ORIGIN      
        1 catcgaggtc tgatagggag cgcacgtcgc cgtgtacaaa ctcaatccca agttgtttaa
       61 aatcaggtaa attaaattca gaccctcgcc gctttaaatt atcaaaagca acgacctggt
      121 tgcttgaact aattttttta aacatctttg ccgccttcat tacgatcgtg atcctggccc
      181 aggatgacgg ggaccgagtg ttggcacgcg cacgcagttt cttcggctgc tatgaggtcg
      241 ttgccagtga acagagaacc ctgcggtatt tgaatcatgg aaacatcgtc catggcggtc
      301 agtgtgtgtc ttcgattctg cgcgatgttc cgatactcta ctatcatcct tccggcccga
      361 ctggtcaggt gctgcagcaa tacaaccggc aacgaaacgt gcgtgtgggc attgtcggca
      421 tgggcgcggg aatggtcgcg gcgtattccc gcttcagcca tgagtatgtg tttttcgaga
      481 ttgacccggt ggtgattcgc attgcccgtg atccgcatct gttcacgtat ctggctgatg
      541 cccgcggaaa gattcgcgtg gtgtgtggcg atgggcgtct cgggctggcg gcggaaccag
      601 aacactcgtt tgatgtgctg atgtttgatg cgtattcctc tgatgcgatt ccggtgcatc
      661 tactgacccg cgaggcgatg cagttgtttc tcagtcgtct gaagccgggc ggtatcatgc
      721 tgtttcactt gtcgaaccgg taccttcgcc tggaaccggt gctcggcaat ctctgtgagg
      781 atctcgagct gaccgccgtg attcaagatc atgagcccga cgagaaaaaa ctgccgccct
      841 cagaagtggg tttggtcagc agctcacgct gggcgttggt ggcctcggaa tctgccaatc
      901 tggtgaaatg gctgcctgat ccgcgttgga agctgctctc gggtgatccg cgcgtcggcg
      961 tctggaccga tcagttttcc agtattttat cggtgtttag aaagtaacag aaatatatcg
     1021 aaaataagcg aaaatagggc aatatagatg catatattct gtcaacttcc tgagatctct
     1081 gccgattctg tgtaaatgcc gaaaatgaga gatctcaatg tcgattcttt acgacgatac
     1141 aaaatgtaga acacgaaaat catcgaagat cgacaagcca ggagtgactg acatgaacgc
     1201 cacgacacag atgcctcgag aagttcgctc agatcgccgc atttccattc cggactgggc
     1261 acggcatctt ccctttggtc agctcaatcc ctggcaaaaa ggacgttaac tacgtgtagc
     1321 gacagaaggg tcgccgagta gcgacggctc cccttcgtta gatttccccc tgaacgtgat
     1381 cggccccggg cctcccaccc ggggccgatc tattttagcg tgtcaggtga tgggttcgac
     1441 cagatgagcg cggttggcag gggcggggta gggctcgggc cagaactcga ccagacgagc
     1501 gatttttcgg tcacggatgg tgaagaacga gatcgcgcgc gcggtttgca cgccatccgt
     1561 gaccgagaca tctgaagccg cctcaagatc tgagccgaac aggcgattga tggtaaaagt
     1621 ccaggttccg tgagccggat actggctgtt catctgagca aagcgttgag cgccacgaat
     1681 tctctcgttt gattgaggcc actcgagcac gaaatcttca tggagaaccg tgccaaccga
     1741 aacgaaatcg ttcgtcgaca tcagacgcca gaactcgcgg acgatctcga cggcagatga
     1801 ctggtcatga ggtgcgggtt tgacttccac gtgggtttca atcctgtccg aaaatgtgta
     1861 ccggtttgtc aacaatgaga atcgctcaaa cagcgatcaa gatggttgcg aaaattgttg
     1921 gcgaaagaaa aaggcacccg ataacctgca ttggtcatcg ggtgccggtg ttgtggtcac
     1981 gctctacggt cagtcggcgg ctccggcctt aatgagcagg tcgatcacgg cctggaagtc
     2041 cttgatttcc tgcggcggat tcgcttcatc gtacttcgga tttttcttgg cgctttcaag
     2101 cttttcggcc agacgctctt tcgcgatctg atgggctttc ttcttgtcag cgttcgccag
     2161 gttgacgttt gccttgagtg agagcagggt ttcaatggtt ttcagcccgc cgttgcgtgc
     2221 cgccagcatg agcggggtgt accctttctg attctgttca tcgatgctcg cacctttttc
     2281 caccaacaac ttgaccgcat cggctcgatt gccgagagcg gcactgtgga gaggagttcc
     2341 gcccaggccc atgacgtttt cggaaatggc cttcagattc gcgccttttt cgagaaacag
     2401 ggcgatggct tcggagttgc cgcgataggc cgcccaatgc agcggggtca tcttgtcgtc
     2461 gttggcgaca tcgatcttgg cacctttttc caagagcagc ttcacgatgt cgatgggcat
     2521 gtgtccgacg gcgttcatga tcggggtgag cccctggttt gcgacgaagt tcacgtctgc
     2581 gcccttgtcg atgagatact tcacgatgtc tggcttggtc gagataatcg cgagcatcaa
     2641 cggtcgatct ttttcaccga tcaacttcga gttggcgttc atctggccgc catcgatcat
     2701 ggcctgtacg gttggcagat cgcctttgat gatggcttca tgaaacttct tcacattctc
     2761 atcgccatcg gcgaaggtcg gcaggcaaca ggcgaaaaga gcgaaaagaa cgagtatgag
     2821 agaaaaacgt ttcaagaggt cctcctgaat gaagtgtgat aaaaaagcga acctagtgta
     2881 tcacatctgc ggcagccgtt cagaatgagt tgtactgatc gtcacctgcc cgcagatcct
     2941 gccacgagac aatgacaaac tgcgtgatat cactggaatt cccgttcacg ccggaggaaa
     3001 cgccaaagcg gggcaaactc tcgtcgtaga tctttttccg gagaggggga gtatagctgc
     3061 cagctttctt ttttacgaac ttgtatgcat atctgacttc actaccatac atgcgatgaa
     3121 tgaatgggtt gtccggacgc tgggaa
//
LOCUS       DAORHD010000497         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35198_length_3144_cov_3.674976, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000497 DAORHD010000000
VERSION     DAORHD010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35198_length_3144_cov_3.674976"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKO06_11065"
     CDS             <1..436
                     /locus_tag="PKO06_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNV70229.1"
                     /translation="DPVRRRQNWGTYAVIDQKLWNRPNKPDQGIGGFVQVGGLTPNDR
                     SLIERYAGVGLNFIGPFRNRPADVFGIAYNVAETSTAFHDFNQAAGTLALDREKVLEV
                     GYRIQVNDWLTVKPNVQFIKNPGADSTRSESRVFGLRFTTIF"
     gene            628..1710
                     /locus_tag="PKO06_11070"
     CDS             628..1710
                     /locus_tag="PKO06_11070"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNV70230.1"
                     /translation="MNTFDIMQHGGLEQIVYFHDESVALKAVVAIHSTQLGPAIGGVR
                     MMEYASQSEVVKEAVDLAQEMTFRAALADCDLGGGAVVLWGNPKTKSEALFRSLGRFI
                     RRLGGQFLAVMEVGTDSRDLRNIRRETDFVYSLPEAYGGIADPTETTAEGVLYGLKAT
                     AKTLFNQQSLAGMTFVVQGVGRLGSAVVKRLVAAKAKLVITDRNYDKIKNIQDVFPEI
                     TMIRPEEVLGTKCDMLVPCALGNLIDAGSIDRLRCKAVAGGASNIIPDLATGDELHKR
                     GITYVPHFVMDAGKLIQADHELKKLSKPLLDSAVGEIYTRTVHLLETAREAKEAPVRT
                     AVKLAWNRLSAIGQIGQRKLKVEAGV"
     gene            1707..2795
                     /gene="buk"
                     /locus_tag="PKO06_11075"
                     /pseudo
     CDS             1707..2795
                     /gene="buk"
                     /locus_tag="PKO06_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780791.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
     gene            2949..>3144
                     /locus_tag="PKO06_11080"
     CDS             2949..>3144
                     /locus_tag="PKO06_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNV70231.1"
                     /translation="MQEALDEFESRKADLIIAIGSPAAQAIKSRRKNLDVPVVCIGCF
                     DPVAMGLAQSYAGSGNNITGN"
BASE COUNT          684 a          922 c          936 g          602 t
ORIGIN      
        1 cgacccggtt cgtcgccgac agaactgggg aacctatgcc gtcatcgacc agaaactctg
       61 gaatcgcccg aacaagcccg atcagggaat cggcggattt gtacaagtcg gtggtctcac
      121 cccaaacgat cgctcgctga ttgagcgcta tgcaggagtc ggcctgaact tcatcggtcc
      181 gttccgtaac cgcccggccg acgtttttgg gatcgcctac aatgtcgcgg agacgagcac
      241 ggccttccat gatttcaatc aggcggccgg gacgttggcc ctcgatcggg aaaaagtcct
      301 ggaggtcggg tatcgaatcc aggtgaatga ctggctcacg gtgaaaccaa acgtccagtt
      361 catcaagaat ccgggtgcag actccacccg gagcgagtca agggtcttcg gccttcgctt
      421 caccaccatc ttctgaatct gccccgcgtt tcgtcggctc ctgttacgcg aacctgccgt
      481 cactttcacg gaacgtcagt attcgggcgg gtcctgaacc ggctgaggga aaccattcaa
      541 gcgtaacggg ggttgtggta ttttaggaga gagaaacacc gctcggcccc agagagattg
      601 ccgggcgtgc ggaaaggaag aatgcccatg aacacgtttg acatcatgca acacggcggt
      661 ctcgaacaga tcgtctactt tcacgacgag tccgtcgccc tcaaggcggt cgtggccatt
      721 cacagcaccc agttaggtcc ggcgatcggc ggggtgcgca tgatggagta cgccagccag
      781 agtgaggtcg tgaaggaagc ggtcgatctg gcccaggaaa tgacttttcg cgcagctctt
      841 gccgactgcg acctgggcgg cggtgccgtc gtactctggg gaaaccccaa gacgaaaagc
      901 gaagcgctgt tccgatcgct cggccgtttc attcgccgac tcggtgggca gtttctcgcg
      961 gtcatggaag tcggcaccga cagtcgcgac ctccgcaaca ttcggcgcga gaccgacttc
     1021 gtctactctc ttcccgaagc ttatggcgga atcgccgatc ctacagagac gacagccgaa
     1081 ggcgtcttgt acggactgaa agcaacggca aaaaccctgt tcaaccagca aagtctggcg
     1141 ggcatgacct tcgtggttca gggcgtaggt cgcctcggtt cagccgtggt caaacgactg
     1201 gtcgcggcga aagcaaaact cgtgataacg gaccgcaact acgacaagat caagaacatt
     1261 caggatgtct ttcctgagat caccatgatt cgccccgaag aagttctggg caccaaatgc
     1321 gacatgctcg tcccttgcgc gctcggcaac ctgatcgacg ccggcagcat tgatcgcctg
     1381 cgctgcaagg ccgtggcagg cggtgcgagc aacatcattc ccgacctcgc gactggtgac
     1441 gagctgcaca agcgtggcat tacctacgta ccacacttcg tcatggatgc cggcaagctg
     1501 attcaggccg atcacgagct gaagaagctc tcgaagcctc tgctcgacag cgcggtcggt
     1561 gagatttaca cccgcacggt gcatctgctc gaaaccgcac gcgaagccaa ggaagctccg
     1621 gtacgcaccg ccgtgaaact ggcttggaat cgcctttctg ccatcgggca gatcggacag
     1681 cgcaaactca aggttgaggc gggcgtatga gcgagaccaa gcagtacctg ttactggtca
     1741 tcaaccccgg ctcgacctca acgaagatcg gggtcttcga gaatgaggtc tgcaagtgtg
     1801 acaagacgct gcgtcacagc accaccgaac tcgagaagta tgagaagatc tgggatcagt
     1861 acgagttcag aaaacgtgaa atcatcgttt ttctgcagaa tgcggggtat gatctggcgc
     1921 agtttcatgc ggtcgtcgga cgcggtggct tgctgcgccc catcgaaagc ggcacctatc
     1981 gcgtcgatca gcacatgatc aacgacctgc gtatcggcgt acaggggcaa catgcttcaa
     2041 acctcggtgg agtgctggcc tacggcatcg gctgggacta ttccattccg tcgtttatcg
     2101 ttgatcctcc ggccgtcgac gaactcgatc cactggcgcg gctttcgggg gtcgccgaca
     2161 ttccgcgcag cagcctcttt cacgcgctca acattcgggc tgtggcccgc aaagccgcca
     2221 cagacatggg aaagcgactg gagcagctga acctgatcgt cgcgcatctg ggcgggggca
     2281 tcacggtcgc cgctctgcgc caggggcggg ccatcgaggt caacaacggc ctgtccgagg
     2341 gtccgttcac cccggaacgc tcaggctccg ttccactgct gccgttgatc gacctctgtt
     2401 tttccggaaa gttcgagaag aaagccattg tgaaaaagct ggtcggtggc ggtggtctgg
     2461 tgagctatct cggcaccaac tcagccagcg acgtcgacgc catggtcaag gccggcaacc
     2521 cgcgcgcccg ccaggtttac gaagccatgg cctatcagat tgcctgtgaa atcgggtcgc
     2581 gcgccgttgc cctggcagga aaagtcgacg ccatcgtgct gaccggcggc atggcgcaca
     2641 acaccatgct caccacctgg atcgaagacc gagtgtcgtt tctcggcaag gtcgtcaagt
     2701 atccgggcga agatgaactc ggcgcgctgg cccagggtgg tttacgcgtg ctgcgcggag
     2761 aagagtccgc caaggtctat aacgcgcaga agaaggtgct gggtatctat cactcggaaa
     2821 cattgctcga atacgatcat gccatcgcgc agatcgagtc caggttgcgc gaagccggat
     2881 tccgcttcag gcaacaggat gagaacatcg agattctcat tcgcaacgga aacggccagg
     2941 cggcgcgcat gcaggaggca ctcgatgagt tcgaaagccg aaaggccgac ctgatcatcg
     3001 cgatcggttc tccagcggcc caggcgatca agtcgcgacg caagaacctg gacgttccgg
     3061 tcgtctgcat cggttgtttc gacccggtgg ccatgggtct ggcgcagtca tacgccggtt
     3121 ccggtaacaa cattaccgga aact
//
LOCUS       DAORHD010000498         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35200_length_3144_cov_3.538038, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000498 DAORHD010000000
VERSION     DAORHD010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35200_length_3144_cov_3.538038"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1516
                     /gene="cysS"
                     /locus_tag="PKO06_11085"
     CDS             68..1516
                     /gene="cysS"
                     /locus_tag="PKO06_11085"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNV70232.1"
                     /translation="MTVSLYNSLTRTKEPFVPHEAGLVGMYVCGPTVYGPPHLGHAKS
                     YLTFDVLVKHLRQRGLNVRYVQNITDVGHLTDNADAGEDKIERQARLDQVHPYEIVDK
                     YMFQYFRDMEALGIGHPNFYSRATQHIPEMIEHVQKLVEKGHAYVVDGNVYFAVESFK
                     HYGKLSGRVLEEQREGVRVESRSDKRSARDFALWKKAEPGHLMKWNSPWGVGYPGWHI
                     ECSVMAQKYLGDTVDIHGGGLENMFPHHECEIAQAEALTGKPFVRFWVHNNMVTVDGV
                     KMGKSLGNFKTIQDLLKTHAPEALRVFLLSTHYRSPTNYTEEAIQAATTGLARLSQAR
                     AALQGAARGTGGKADFDTEVTRLCEEANRQIGDALDNDLDSPGAIAHLYNFVSELNRV
                     MTRGTLTSKAAQDALTLLKKWGEDVLGIVKVSAGESKAADLTPIMDLLIEIRKELKAA
                     KRFDLTDQIRDRLKAAGILIEDTREGARWRLA"
     gene            1513..1980
                     /locus_tag="PKO06_11090"
     CDS             1513..1980
                     /locus_tag="PKO06_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNV70233.1"
                     /translation="MIPRLATPWERERVVRLPARTLAYVGDSVFELCLRLWHVAERSD
                     PAGKLHESVVGLVCADRQSELFERVFPTVSEEEQVLLKNWRNAKMPSRHGGGSRGAYA
                     RATAFEAWIGYLYLTGQSERLEQTVALVHTTPEPAAAAPSYVVRPVRKGEDEK"
     gene            1977..2990
                     /gene="rlmB"
                     /locus_tag="PKO06_11095"
     CDS             1977..2990
                     /gene="rlmB"
                     /locus_tag="PKO06_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNV70234.1"
                     /translation="MKRSGGRPYSQAAVRRDHSPGSGRSPRVERGERPWAEAKPPRRD
                     ERPGAADRRRDDPHSPRRDSHRADRQGFVSRDRGAPPPREEALDGPIWIRGRHEVLAF
                     LKSGKPVESLLMALEARGKEIEEIKALARARAIKVQALPMTGFRQKVGSESQGVALRI
                     GAFPYADFETLLQDALRENGLLLVLNHVEDPRNLGAIVRTAEAAGVAGVVIPKNRAAG
                     MTEWAIRTSQGAAAHLPVARVTNIGVVVERLKQEGFWCYGLDEDAVGRYDEIRYNARM
                     ALVIGGEDAGLGEKVKGLCDECVRIPMLGKTPSLNVAVSAGIVLFEVLRQRGFPPKKT
                     AAN"
BASE COUNT          645 a          842 c         1003 g          654 t
ORIGIN      
        1 gacttgttgt tttgtgctat catcgggcgt tcaatccagt cttcattccc gcgtcaccgg
       61 aggacgtatg acggtatctc tctacaactc tctcactcgc accaaagaac catttgtacc
      121 gcatgaagcc ggcctggtcg gcatgtatgt ctgcggaccg accgtctacg ggccgcctca
      181 tctgggtcac gccaaaagct atctgacgtt tgatgttctc gtgaaacatc tgcggcagcg
      241 aggtttgaac gttcgatatg tacagaacat caccgatgtc gggcacctga ccgacaatgc
      301 cgatgcgggc gaagacaaga tcgagaggca ggcgcgcctc gatcaggtgc atccgtatga
      361 gatcgtcgac aagtacatgt tccagtattt ccgtgatatg gaagcgctgg gcatcggtca
      421 tccaaacttt tacagtcgtg cgacacagca tattcccgaa atgattgagc atgtgcagaa
      481 actggtcgag aagggccatg cctacgtcgt cgacggaaat gtctacttcg cggtcgagtc
      541 gttcaagcat tacgggaagc tctcggggcg tgtgctcgag gagcaacgcg agggagtacg
      601 agttgagtct cgatcagaca agcgcagtgc gcgcgatttc gcgctctgga agaaagccga
      661 acccggtcac ctgatgaaat ggaattcccc gtggggtgtg ggatatccag gctggcacat
      721 tgaatgctcg gtcatggcgc agaagtatct cggcgatacc gtggacattc acggtggtgg
      781 tctcgaaaac atgtttccgc accacgaatg cgagattgcg caggccgaag cgcttactgg
      841 caagccgttc gtgcgcttct gggtgcataa caacatggtg acggtcgatg gggtgaaaat
      901 gggcaagtcg ctcggcaact tcaagactat ccaggatctc ttgaaaaccc acgcaccgga
      961 agccttacgg gtctttctcc tgtcgaccca ctatcgctcg ccgaccaact acaccgaaga
     1021 agcgattcag gcggcgacca cgggcctggc tcgtttgtca caggcccggg cagctctgca
     1081 gggagcggcg cgcggcacgg gcgggaaggc cgatttcgat acggaagtga cgcggctgtg
     1141 cgaagaagcc aaccggcaga tcggtgacgc cctcgataac gacctcgatt ctcctggggc
     1201 gattgcccat ttgtacaact ttgtttctga actcaaccgc gtcatgactc gtggaaccct
     1261 gacgtcgaag gcagctcaag atgcgctgac cctgctgaaa aagtgggggg aagacgtgct
     1321 cggaattgtg aaagtcagtg ccggtgagag caaagccgcg gatctgaccc cgatcatgga
     1381 tctgttgatc gagattcgca aagagctgaa ggcggcgaag cgtttcgacc tgaccgatca
     1441 gattcgcgat cggttgaagg ctgcgggtat tctgatcgaa gatacgcgcg aaggcgctcg
     1501 ctggcggctc gcatgattcc gcggctcgcg accccctggg agcgcgagcg ggtcgtgcga
     1561 ctgccggccc gtacgctggc ctacgtcggc gactcggtgt tcgagttgtg cctgaggctg
     1621 tggcatgtcg ctgaacgctc tgaccctgcc ggtaagctgc acgagtccgt ggtcgggctg
     1681 gtgtgtgctg atcgtcagtc cgagttgttt gagcgtgttt tccctacagt gagcgaagaa
     1741 gagcaggtgc tcttgaaaaa ctggcgtaat gcgaagatgc cttcccggca cgggggaggc
     1801 tcgcggggcg cttatgcccg ggcgacggcg ttcgaagcct ggatcggata cctgtatctg
     1861 accggacaga gtgagcgtct tgaacagacc gtggcgttgg ttcataccac cccagaaccg
     1921 gctgcagctg ctccttcgta tgttgtgcga ccggtacgaa aaggagaaga cgagaaatga
     1981 agcgttccgg cggtcgtccc tattcacagg cagctgttcg ccgcgatcat tctccgggat
     2041 ccggccgttc gccccgagtc gagcgcggcg agcgcccctg ggcggaagca aaaccgcctc
     2101 gacgagatga gcgccccggt gcggctgatc gtcgcagaga tgatccgcat tcgcctcgtc
     2161 gcgattccca ccgggcggat cgacaggggt ttgtgtcgcg tgaccgtggt gcgccaccac
     2221 ctcgggaaga agcgctcgac ggcccgatct ggatccgcgg tcgtcatgaa gtgctggctt
     2281 ttctcaagtc aggcaaaccg gttgagtcgc tgttaatggc tctggaagcc cggggaaaag
     2341 agatcgagga gatcaaggct ctggctcgcg cgagggcgat caaagtgcag gcgttgccga
     2401 tgaccggctt tcggcagaag gtcgggagtg agagccaggg cgtcgccctg cgtatcggag
     2461 cttttccgta tgctgacttt gaaacgctgt tgcaggatgc tttgcgtgag aacgggctgc
     2521 tgctggttct caaccatgtc gaagaccccc gcaacctcgg agcgatcgtc agaaccgccg
     2581 aggcggccgg ggtggcgggc gtggtcattc ccaagaaccg ggcggccggc atgacggagt
     2641 gggcgatccg tacctcgcag ggggcggcgg cacatctgcc tgtggcgcgg gtgaccaaca
     2701 tcggtgtcgt ggtcgagcgt ctgaaacagg agggattctg gtgctacgga ctcgatgagg
     2761 acgcggtcgg cagatatgat gaaatacggt acaatgcgcg aatggcactg gtcatcggcg
     2821 gtgaagacgc cgggcttgga gaaaaggtga aaggtctgtg cgatgagtgt gtacgtatcc
     2881 cgatgctggg gaaaaccccg tccctgaacg tcgcggtgag tgcgggaatc gtgctgtttg
     2941 aggttttgcg gcagcgcggt tttcccccga aaaaaactgc tgcaaactag ttgacttttc
     3001 ctcttttctc ggctaacata gcatttcacc gcccaccttg agcggtacgt tcttttctaa
     3061 ctcaatcgtg atgccggcgt agctcaattg gcagagcagc tgatttgtaa tcagcaggtt
     3121 gcgggttcga gtcccatcac cagc
//
LOCUS       DAORHD010000499         3142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35237_length_3142_cov_3.579851, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000499 DAORHD010000000
VERSION     DAORHD010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35237_length_3142_cov_3.579851"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKO06_11100"
     CDS             complement(<1..182)
                     /locus_tag="PKO06_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70235.1"
                     /translation="MGRPKQHVMLAGRCFLDHILERLRAVRNWLGTVIMIGREGDDAG
                     RQRAVDFGALWVENPHP"
     gene            363..1655
                     /locus_tag="PKO06_11105"
     CDS             363..1655
                     /locus_tag="PKO06_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70236.1"
                     /translation="MAAVDFKSIIPKEPESFIDLKVDLEIITNLIMKELYVHGKKTPR
                     QIAKTLKIRQEIIQKIINDLKQQEFVGMVGRTGVGSDYQYGLYPKGTERAKQIYEQDT
                     YLGPAPVGFEEYTRVTKAILAANKAGFNINFKVLQEKFGHYLGYKDVLFQIGQAVCAR
                     KPAFVYGFPGNGKTYLCSSVVKLLPPMVIPYAVDVGNQLVRIFDPSCHIPLESIDLEG
                     FDQRYLVVQPPMIIAGGELTLEDLEVKYNKKFGCFDAPPQVKANGGVFLIDDLGRQRC
                     EVEEIFNRFIIPLENKVDFLVLGGQRMEVLTDEIVLFSTNLDPMKIVDDAFLRRLPYK
                     INVRNPTTEEYADLWKTYADKVGLKFDPVNIAFITTLYKRDKRGLRTVHPRDILTIIR
                     DRKRFQEDANTAISEQEITEAYGIYFVSQLTLVETFQD"
     gene            1652..2530
                     /locus_tag="PKO06_11110"
     CDS             1652..2530
                     /locus_tag="PKO06_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV70237.1"
                     /translation="MNFLQLLINLGAGAMAGHAAWLSFRSGIPWSFGDNFLATVLTCG
                     VFSSLFLGLGRALPALILDHRPRRTMETFASAAAIGLAIPMTAGVLYSLAMDTLHMGG
                     LMPYLLRRALWWSVFAVSLAISRGLIIGNPVSGCRAFIGLIPGFLFGGLALDFFFLTR
                     DLNWLGSLWLGLCAGLTLSLALDLLKESWLEEFSPRTLRRQFLLETDDFVVGTDDLCD
                     LVLDSGPEQWLLITEKDGMHTIEVLDENVPVIIGTCRFRYHVLHDGDALQLGERVFIY
                     HTRYARSRDILPEACS"
     gene            2589..2996
                     /locus_tag="PKO06_11115"
     CDS             2589..2996
                     /locus_tag="PKO06_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70238.1"
                     /translation="MITSHRWLVLFLCLSSLTLLCANVVPVCAQEPGTTGDPLVSKSY
                     LDQFFRFHSLVVPGGQKLPLQQGALLVLRSGKCRIVGPKGKALLDLTDGKEIPAGAVL
                     PPLHLILVADAGDLQLEAQAMCQFMAQGLRPRK"
BASE COUNT          611 a          962 c          844 g          725 t
ORIGIN      
        1 gggtgcgggt tctcgaccca gagcgcgccg aagtcgaccg cccgttgcct cccggcgtca
       61 tctccctctc ttccgatcat gatcaccgtc ccaagccaat tgcgaaccgc acgcagacgt
      121 tccagaatat ggtcgagaaa gcaccgccca gccagcatca catgctgttt tggacgaccc
      181 atgcgtcgcg aatcgccggc aagaagaaca agacatccgg gtttcatcaa cccaacaccc
      241 cctgtttgcc agcttcacat ctgctctttg agcccagacg cgctccgcaa tccttcattg
      301 cattagccgg ccaaagttgg taccatgcat ctgttgtatc agatatcgtg aggaggaacc
      361 acatggctgc agtcgatttc aaatccatca ttcccaaaga acccgaatct tttatcgatc
      421 tgaaggtcga tcttgagatt atcaccaacc tgatcatgaa ggaactgtac gttcacggca
      481 agaagacccc gcggcagatc gccaagacgc tgaagattcg ccaggagatc attcagaaga
      541 tcatcaatga tctcaaacag caggagttcg tcggtatggt cggcagaacc ggcgtcggtt
      601 ccgattatca gtatggtctg tacccgaagg gcaccgaacg cgccaaacag atttacgagc
      661 aggataccta tctcggtccc gcaccggtcg gcttcgaaga atacacccgg gtgaccaaag
      721 ccattctggc cgcgaacaag gccgggttca atatcaactt caaggtgttg caggaaaagt
      781 tcgggcacta cctcgggtac aaagacgtcc tgtttcagat cggtcaggcg gtctgcgcgc
      841 gcaagcccgc gttcgtctac ggctttcccg gcaatggaaa gacgtatctc tgttcgagcg
      901 tcgtcaaact gctcccgcca atggtcattc cctatgcggt cgatgtcggc aaccagctgg
      961 tacgcatttt tgatcccagc tgccacatcc ccctcgagag catcgatctg gaaggatttg
     1021 atcagcgcta tctcgtcgtg caacccccga tgatcatcgc cggcggcgaa ttgacgctcg
     1081 aagatctcga agtcaaatac aacaagaagt tcggttgctt cgacgcgccg cctcaggtca
     1141 aggcgaacgg cggcgtcttc ctcatcgacg atcttggtcg tcagcgctgc gaggtcgaag
     1201 agatcttcaa ccgtttcatc atccctctgg aaaacaaagt ggactttctg gttctcggcg
     1261 gccagcgcat ggaagtgttg accgacgaaa tcgttctctt cagtacgaac cttgacccga
     1321 tgaaaatcgt tgatgatgcg tttctacgcc gactccccta caagatcaac gtgcgcaacc
     1381 ccacgaccga ggagtacgcc gacctctgga aaacgtatgc ggacaaagtt ggcttgaaat
     1441 ttgatcccgt caacattgcc ttcattacaa ccttgtacaa acgcgacaag cgcggtctcc
     1501 gcacggtgca tccgcgtgac atcctcacca tcattcgtga tcgcaaacgc tttcaggagg
     1561 acgcgaatac ggccatcagc gaacaggaga tcacggaagc ctacggcatc tacttcgtgt
     1621 cccagctcac cctcgtcgaa accttccagg attgaacttt cttcaactgc tgatcaacct
     1681 cggagccggg gcgatggccg gccatgccgc atggctgagc tttcgctccg gcattccgtg
     1741 gtcgttcggc gacaattttc tggccacggt tctgacgtgc ggggtgttct cgtctctctt
     1801 tctgggcctc gggcgggcac tgcccgccct gattctcgac catcgtccgc gccgcaccat
     1861 ggaaacgttc gcctcagccg cggcgatcgg actggcgatt cccatgaccg ccggagttct
     1921 ctactcactg gcgatggata cgctgcacat gggcggtctc atgccgtatc ttctgcgacg
     1981 cgccctgtgg tggagcgtct tcgccgtttc cctcgccatc tcgcgcggcc tgatcatcgg
     2041 caatccggtt tcgggctgcc gcgcgttcat cggtctgatt cccggttttc tgttcggggg
     2101 gctggcgctc gacttctttt ttctcacccg cgatctcaac tggcttgggt ctctctggct
     2161 cggattgtgc gccgggctga ccctgtctct cgctctcgat ctgctcaagg aaagctggct
     2221 cgaggagttt tcgccgcgaa cattgcggcg tcagttcctg ctcgaaaccg atgatttcgt
     2281 cgtgggcacg gacgatctct gtgatctggt gctcgactcc gggcctgagc agtggctgtt
     2341 gatcacggaa aaggacggca tgcacaccat cgaggtgctc gacgagaacg tgccggtcat
     2401 catcggcacc tgtcgttttc gctatcacgt gctgcacgat ggagatgcgc tgcagctggg
     2461 tgagcgggtg ttcatctacc acacgcgcta tgcccgcagc cgcgacatcc tgccggaagc
     2521 ctgttcctga gttttcgtct ctcccgtcgc ttgctctctg gagatgagct tggtacaatg
     2581 ctggcgctat gatcacctct catcgctggc tcgtgctgtt tctgtgtctc tcgtcactga
     2641 cgcttctctg cgccaacgtc gtcccggtgt gtgcccagga acccggcacc accggcgatc
     2701 cactggtttc caagagctat ctcgaccagt tctttcgctt tcactcgctc gtggtgcccg
     2761 gtggccagaa gctgccgctg caacagggag cgctgctggt cctgcgttcc gggaagtgtc
     2821 gtattgtcgg tccgaagggg aaagcgctgc ttgacctcac agatggcaag gaaattccag
     2881 ccggagcggt tcttccaccg ctgcacctga ttctggtcgc tgatgcaggc gacctccagc
     2941 tcgaagccca ggccatgtgt cagttcatgg cgcagggtct cagaccgcgc aagtaacgtc
     3001 gttcatgtct tccgtacgat ctcgtctcgg tcgcctgctg gcggccatgc tgcttttgcc
     3061 agggttgttt ctcgcctggc aacgctgtca gctgcgtctc gatgtcgaga gtcgcgctcg
     3121 ccacatcgaa acggtcatgg at
//
LOCUS       DAORHD010000500         3140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35266_length_3140_cov_4.678444, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000500 DAORHD010000000
VERSION     DAORHD010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35266_length_3140_cov_4.678444"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKO06_11120"
     CDS             complement(<1..287)
                     /locus_tag="PKO06_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70239.1"
                     /translation="MKRTCFVFVIVVVGAFALFMLMAASNVTYDYGVDKDTTKLPFRT
                     YYDGEKQKRYDFCTVLFSVQKDLATGSYREFTVSLAHLEYQSQGVHRGKYVS"
     gene            689..1720
                     /locus_tag="PKO06_11125"
     CDS             689..1720
                     /locus_tag="PKO06_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70240.1"
                     /translation="MKIRHQSFRGKCLFCGARTQNPDPSPAVTREAPEDILWRPERKS
                     TGSGTGTTTATSPATSLPSGRTAGSSSLATDSRQSERPSPPSNGIWHTTAGPIIEDLY
                     DDCPQARFHIILFSVVIILPLLFLTFDLPIIAGGLVLGILFFKFQYTAEQGKWYGLRD
                     VQSEYESLLATASFNNRNYYNLLLGPALSSLLSLRDRAFKARSLILGESFEEIDYKIN
                     WIENRARNLDDPDLSDMYQSQVKDLKENRKKLEQISILLERFEASKNCMVESIKLLKS
                     RILLSETRDDRVEEAQIREDLKSLHFVYERINHPHLSPTESETTLTTEHQETLPTTCA
                     SPVRAVLHS"
     gene            2000..>3140
                     /locus_tag="PKO06_11130"
     CDS             2000..>3140
                     /locus_tag="PKO06_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV70241.1"
                     /translation="MRSASIAGRTFPITVLISILISIAGWSPRVLAVEPREWTVMVYL
                     CADNNLDELGLDNLQEMADAGSTANRSIVALLDRFNAPATEYVIEGRKAVVLRERGEL
                     NMGDPAVLTGFVQDTIARYPAQHYALILFNHGTGWRNRSDGWRGIAYDDSSGDHLSTA
                     ELQTVTADIARHLGRNLDLLGFDACLMQMIEVAYAVRQSVDHVVGSEELEPQYGYPYD
                     SILKALISGMSPGDLARTIVRKYENFYSTRSSKTAYRPVKNQRDPEGTTQSAIKCSRL
                     NDLRASLDAVCAELMKGDHIDRVQQAIARAQRFCTSTHADLGDFLVALKQADNPAPEL
                     DVAIDTALRTLNETVLANAVTQNDASGDWGGESRRWVRQRPVRAFR"
BASE COUNT          740 a          940 c          837 g          623 t
ORIGIN      
        1 gaaacgtact ttccgcggtg aacgccctgt gattggtatt ccagatgcgc aagggaaacc
       61 gtgaactcgc ggtaactccc tgtagccaga tctttctgca cggaaaacag tacggtgcaa
      121 aaatcataac gcttctgttt ttccccgtca tagtatgtcc gaaacgggag tttggtggtg
      181 tctttatcca ccccgtaatc gtacgtaacg ttgctcgcag ccatgagcat gaataacgcg
      241 aaagctccca ccacgacgat cacaaaaaca aaacaggttc tcttcatacg gtcacggctc
      301 cacaattccc gaaggggtcc gacttgatcc cagatacttt tcggttcggt tcaactggta
      361 caaaggtcca catcgtcgtc aatcatcggg ttacagcata ccacacgtaa ccctcagcac
      421 agagtcagag ccacgcaaaa aaatccggca ggtatcttca tggaattgag agaggcgcga
      481 gcggaaacat gtgcaatact gtgcgaactt cggcacacag gagatttgtc aggcaacgtc
      541 tcaagggcgg gcaggtcgcg agaaacccta tcctggagcg gcaatcgatc acagtctcag
      601 acgaacatcc tctgtgcggc tgagggtggc attgttcctg ctgatcatga catgagcgca
      661 gtcgaaacag gaaacacagg aggccgctat gaaaatccgg caccagagtt tcagaggcaa
      721 gtgcctgttt tgcggagcca ggactcagaa tcccgaccca tcccccgccg tcacccgaga
      781 agcccccgag gatattctct ggcggcccga gagaaagagc acaggctcag gaacaggcac
      841 gaccacagcg acaagccccg ccaccagcct gccatctgga agaactgccg gctcgtcgtc
      901 tctcgccacg gactcgagac agagcgaacg cccttccccg cccagcaacg gcatctggca
      961 caccacggcg ggcccgatca ttgaggacct ttacgatgac tgcccgcagg ctcgttttca
     1021 catcatcctg ttctcagtcg tcatcattct tcccctgctg tttctcactt ttgatctacc
     1081 gatcatcgct ggtggacttg tacttggcat cctgtttttc aagtttcagt acaccgcgga
     1141 acaggggaag tggtacgggc ttcgggacgt tcagagcgaa tatgaaagct tgctggcgac
     1201 agccagcttc aataaccgga actactacaa cctgctcctg ggccccgcgt tgagttctct
     1261 cctttccttg cgcgatcgcg ctttcaaagc ccgttccctc attctgggcg agtcgttcga
     1321 agaaatcgat tacaagatca actggatcga aaaccgggcc cgcaatcttg acgacccgga
     1381 tctatccgac atgtaccagt cgcaggtgaa agacctgaag gaaaaccgga aaaagctcga
     1441 acagatctcg atcctcctcg aacgattcga agccagcaaa aactgtatgg tcgaatcgat
     1501 caaactgctg aaaagccgga ttctcctcag cgaaacacgc gacgacagag tggaagaagc
     1561 ccagattcgc gaagatctga agtctctgca ttttgtctat gagcgcatca accacccgca
     1621 cctgagcccc accgaaagcg agacaaccct gaccacggaa caccaggaga cactgccgac
     1681 gacctgcgca tctcctgtcc gggcggttct tcacagctga acgtcatcga gacttgaact
     1741 tcaggacttc ccttcgatcc ccagaattct gcccagaacc ccagatgaag ggcgcactcc
     1801 ctgccgcgac ggcgaatcgt tgagcgatgc caaggggaaa acccaagccg gagaatcaat
     1861 tgcccctggg tgagggcctc gaatcgttga catcacggcc cctccatggg tatgctcgac
     1921 gtgcaggcgg gtaaacattc gcagggataa tcatcgggcc tgtcccgacc agacgagaaa
     1981 gggcgaagag gaaatgtgcg tgcggagtgc ttcgatcgcg ggaagaacct tcccgatcac
     2041 tgttctgatc agcatcctca tctccatcgc tggttggtcg ccacgggtgc tggcggttga
     2101 accccgcgag tggaccgtca tggtctacct gtgcgccgac aacaacctgg acgaactcgg
     2161 actggacaat ctgcaggaaa tggcggatgc cggctcgacg gccaaccggt cgatcgtggc
     2221 gcttctcgat cgtttcaacg cccccgccac cgagtatgtc atcgaaggca gaaaggccgt
     2281 ggtcctgcgt gagcgcggcg aactgaacat gggcgatccg gcggttctta ccggatttgt
     2341 acaagacacg atcgcgcggt atccggcgca gcactatgcc ctgatcctgt tcaaccatgg
     2401 aaccggctgg cgaaaccgtt ccgatggatg gcgcggcatc gcctacgatg attcgtccgg
     2461 cgatcatctc agcactgccg agcttcagac cgtcacagcc gacatcgccc gtcacctcgg
     2521 ccggaatctc gaccttctcg gcttcgatgc ctgcctgatg cagatgatcg aagtcgcata
     2581 cgcggtgcgg caatcggtcg atcacgtggt gggttccgag gaactggagc cacagtacgg
     2641 gtatccgtat gattcgatcc tgaaagccct gatctctggt atgtccccgg gcgacctggc
     2701 tcggacaatc gttcgaaaat acgaaaactt ctattcgacc cgctcatcga aaaccgcgta
     2761 tcgaccggtg aagaaccaac gagacccaga gggaaccacc cagtctgcga tcaagtgttc
     2821 ccgccttaat gatctgcgtg ccagcctcga tgcggtatgc gcggagttga tgaagggcga
     2881 tcacatcgac cgggtgcaac aggccatcgc ccgggcccag cgtttctgca cctcgaccca
     2941 cgctgatctg ggtgactttc tcgtggcttt aaaacaagct gacaatcccg ctcctgagct
     3001 tgatgtcgcc atcgacacgg ctctacgcac cctgaatgag acggttctgg ccaacgcggt
     3061 cacgcagaac gatgcttcgg gcgattgggg cggcgagagc cgcagatggg ttcgccaacg
     3121 ccccgttcgt gcattccgag
//
LOCUS       DAORHD010000501         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35362_length_3135_cov_3.523377, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000501 DAORHD010000000
VERSION     DAORHD010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35362_length_3135_cov_3.523377"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="cpaB"
                     /locus_tag="PKO06_11135"
     CDS             complement(<1..658)
                     /gene="cpaB"
                     /locus_tag="PKO06_11135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HNV70242.1"
                     /translation="MNRRPLLFAMIVSLIISYFMYKNLMQKQQLGTGQLPPAEALIPK
                     IPTKPVVVAKVRIPAKTRLEDPYIREALEIKQMNASDVPIGAFDNVASVTDRYTAITI
                     LPKDVITTERLLDKKLVPNLSFAIPEGKRAYTVMVDKVRGVAGFVQQGDIVDIVGFFK
                     QGGDTEPVSRIVLQDLPVLAVGQTYEFDSAVATTAPTINAAKFDLVTLAVDPTHVEYL
                     S"
     gene            complement(845..>3135)
                     /locus_tag="PKO06_11140"
     CDS             complement(845..>3135)
                     /locus_tag="PKO06_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70243.1"
                     /translation="DGLEVIPLTIPYGVVKDLSKATYSYEPFSDLGQDGFATGTEYIL
                     KLGSGNSGKTTAKRILVPMDDGAQGPKGSSAAVSSGDMTNIGFLKAYGAAYWCLKNGY
                     TPVEWLIGYRGGSFFLPDVPAIETKLGAMGVKYEKKEGIDLINDILKQCKVIELFNYP
                     VVKVYSSQADADAVEKVIQWCEIPYTKTYDKEILEGALDACNWLHMHHEDFTGWTGGC
                     ERLNTSCYTYESYLSKTGKDNNQLCSYCRDYYFGYVPSKAAWGSSTYWGWNGTNTTSK
                     SKTVRISGVNYTINWSKTACKNYAVRCAERATYTGTRWWSINSSDLLCKDGSERPMCQ
                     SAKEAYDLATTKGYNADATGYLQSRQWLNGGSLIKMDGTNPMPLNTDFIYKPNRVQLM
                     KFDVVKKIMDHVTAGGYIFSQCFAPETLDMTIYHRRLYLDKPASPGQAKNYDECFAFS
                     GFTNKCFCRKDSGSGMYYSSINSTLSGEFNPSDTTDPRCQNHGTTKPNFGSGHCDSFS
                     KAAVKNTTDVMAYLTSNANAAQYVKGKYGNGEFCFAGGHYHNNIQSQRLVANNILLGA
                     LSTKELSEGDDTDYTGKQKNNYGVVDPDNVNAGGANDYRDRFMNGFSQPLTIGDRLMN
                     ESGNMSGPTDEAVDFRTEFDATATQTRRFVIVPIHDIPPEVGVNNTKNATASAIYDLQ
                     GTDHPNGEYKPASAGGYDFGASIRIIGFAKFEIIPPEEYARDGGTIVAGDQGDLGFYQ
                     SGQVRGKFVEWVIKPGEVPVPY"
BASE COUNT          646 a          839 c          892 g          758 t
ORIGIN      
        1 gcgacaggta ttcgacgtgg gtcgggtcga cggccagcgt gaccagatcg aacttggcgg
       61 cgttgatcgt cggagccgtg gtggcgaccg cggaatcgaa ctcgtaggtc tgaccgaccg
      121 cgagcaccgg cagatcctga aggacgatgc ggctgacggg ttcggtgtct ccaccctgct
      181 tgaagaaccc gacgatatcg acgatgtcgc cctgctggac gaacccggcg acaccgcgca
      241 ccttgtcgac catcaccgtg tacgcgcgct tgccctcggg aatggcaaac gacagattcg
      301 gcacgagctt cttgtcgagc agccgttcgg tcgtaatgac gtccttcggc agaatggtga
      361 tcgccgtgta ccgatcggtc accgacgcga cattgtcgaa cgcgccgatc ggaacgtcag
      421 aggcgttcat ctgcttgatc tcgagcgcct cgcgaatgta agggtcttcg aggcgggtct
      481 tggcgggaat gcggaccttc gccaccacga ccggcttcgt gggaatcttc ggtataagcg
      541 cctccgcggg cggcaactgg ccggtgccaa gctgctgctt ctgcatgagg ttcttgtaca
      601 tgaagtacga gatgatcaag ctcacgatca tggcgaaaag taagggcctt ctattcacaa
      661 ctgatcctcc tcccgaggga attgacggtc gatcatacca cactgcgctg gtgtacgcaa
      721 ttttgttctg cccatcgaga tgggtgaacg agttgtaaat aataaacgcc atttggcgga
      781 aagcaaggaa aaagttgaaa aaaaagcgcc ggggtcaccg gcgctcttct gaaccgaagt
      841 agaatcaata gggtacagga acttctcctg gcttgattac ccattccacg aattttccgc
      901 gaacctgacc ggactggtag aaccccaggt cgccttgatc tccggcgaca atggtgccgc
      961 cgtcgcgggc gtattcttcg ggcggaatga tctcgaactt ggcgaacccg atgatgcgaa
     1021 tcgaagcgcc gaaatcatat cctccggcgg atgcaggttt gtattccccg tttggatgat
     1081 cggttccctg caggtcgtag atggccgacg ccgtcgcatt cttggtgttg ttcaccccga
     1141 cttcgggcgg aatgtcgtga atcggcacga tgacgaaacg tcgggtctgg gtggcggtgg
     1201 catcgaactc ggtgcggaaa tcgacggctt cgtcggtcgg gccagacata ttgcctgact
     1261 cgttcatgag gcggtcgccg atcgtcaacg gctgactgaa cccgttcatg aagcggtcgc
     1321 gatagtcgtt cgcgccaccg gcattgacgt tgtcgggatc gacgacgccg tagttgttct
     1381 tttgcttgcc cgtgtaatcg gtgtcgtcac cttctgaaag ctctttggtc gacaacgctc
     1441 ccagaagaat gttgttggcg acgagtcgct ggctctgaat gttgttgtga tagtggccac
     1501 cggcaaagca gaactcgccg ttaccgtatt tacccttgac atactgagcc gcattggcgt
     1561 tcgaggtcag ataggccatg acgtccgtgg tgttcttgac ggccgccttt gagaacgagt
     1621 cgcagtggcc gcttccgaag ttcggcttgg tcgtaccatg attttggcag cgcgggtcgg
     1681 tcgtgtcgct cgggttgaat tcgcccgaga gcgtggagtt aatgctggaa tagtacatac
     1741 ccgaaccgct gtctttacga cagaagcatt tattcgtgaa tccactgaat gcgaagcact
     1801 cgtcgtagtt cttggcctgt cccgggctgg ctggtttgtc gagatagagg cggcgatgat
     1861 agatcgtcat gtcgagcgtt tcgggggcaa aacattgcga gaagatgtag ccgcctgcgg
     1921 tcacatgatc catgatcttc ttcacgacgt cgaacttcat cagttgcaca cggttcggct
     1981 tgtaaatgaa gtctgtattg aggggcatcg gattagtccc atccattttg atgagcgatc
     2041 ctccgttcag ccactgacga ctttgcagat accctgtcgc atcggcgttg tagccttttg
     2101 ttgtagccag gtcgtaggct tcttttgcag actgacacat cgggcgctct gagccgtcct
     2161 tacacaggag gtccgaagag ttgatgctcc accagcgagt acccgtgtag gtcgcgcgtt
     2221 cggcgcaacg tactgcatag ttcttacaag cggtcttgct ccaattaatc gtgtagttaa
     2281 cgccgctgat tcgcacagtc ttcgattttg acgtggtatt ggttccgttc caaccccagt
     2341 aggttgatga gccccaagcg gcctttgaag ggacatatcc gaaatagtag tctcggcaat
     2401 aactgcagag ttggttgttg tctttcccag tctttgacag ataactttca tacgtgtaac
     2461 agctggtgtt cagtcgctcg cagccgccgg tccagccagt gaagtcctca tgatgcatat
     2521 gcagccagtt acaggcatcc agagcgcctt cgagaatctc cttgtcgtag gtcttggtgt
     2581 acggaatctc gcaccactga atgaccttct caacggcgtc agcgtcagcc tgcgacgaat
     2641 agaccttcac cacaggataa ttaaagagtt cgatgacctt gcattgtttc agaatatcat
     2701 tgattaagtc gatcccttcc ttcttctcat acttgacgcc cattgctcct agttttgtct
     2761 ctattgcagg gacatcgggg agaaagaacg aaccgccgcg atacccgatc agccattcga
     2821 ccggcgtata gccgtttttc aagcaccagt aggccgcgcc gtaggctttc aggaacccga
     2881 tgttcgtcat gtcaccgctc gagaccgctg cggagctccc tttcggtccc tgagcaccgt
     2941 catccatcgg cacgagaatg cgcttggccg tggtcttgcc cgagttgcct gatccgagct
     3001 tgagaatgta ttcggttccg gtcgcgaaac catcctgccc aaggtcagag aatggttcgt
     3061 aagaataggt cgccttcgat agatctttta caacaccgta gggaatggtc agcggaatga
     3121 cctcgaggcc gtcgt
//
LOCUS       DAORHD010000502         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35417_length_3132_cov_4.551186, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000502 DAORHD010000000
VERSION     DAORHD010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35417_length_3132_cov_4.551186"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKO06_11145"
     CDS             complement(<1..424)
                     /locus_tag="PKO06_11145"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1009 domain-containing protein"
                     /protein_id="HNV70244.1"
                     /translation="MSRIGLIAGYGRFPLYFADAARKAGNEVIAVGIREETSPELEPL
                     VEQLHWFNVGQLQSMIDVFLREGVNRLVMAGKITKTRMFQELQPDTRLLKVFEKLPIR
                     NDDALLKALAGEFVAEGIHVCDSTTFLDALLPRQGTLTK"
     gene            complement(614..>3132)
                     /locus_tag="PKO06_11150"
     CDS             complement(614..>3132)
                     /locus_tag="PKO06_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV70245.1"
                     /translation="GQLLAAAQRHRETQTLRRFGAQAQLLAEHLLGPLVTLRDLRESL
                     FPGIFVAHTNAFPESRSIWFRMPERGPGMLVQFEESFFRQGQYWPIPLRHLNRHLRGG
                     IRLGWTSFWQSENNSGMAVPAATPFSHELEAAIAQFRQSQETEIDTPHYLVVCRAMPQ
                     HGVLFAFQPLVRLHERSNTTRRVWHVLLIGLFLTLSLWGFRAFVQERVQFVSIRWQIS
                     AAFLFATSLPLTGMGFLAHEYLQHRESDLRNSVFLRSEQFLRQIDSRFSSTLLETQTA
                     FASLTATVSSSLRPQTTFSRSPILASGIAQLNTSEFYLVNDDETPILTQEKLLPTGRP
                     LSSGVDKEQRLFSAVLAALFRDLTGTAANPRKNLALNELIEDFMGRESTEEFRNTMIL
                     QLGRISLFSNMDKRVVIMLSPLTEGLAGPLRYLALAQWSQWKLQRQHILRTRLQAARD
                     RRAPRFFAVTTHSFQLVPENTPDPRPLNAFSERVRREQRTVMDAIGWNGRSWLAVGVP
                     GQHLSSHHLLALTPQTVVEKRMLRLKRLLLTIALLALLITIMIATVLSRSILMPIQRL
                     AAATTAIERRQFDFRVTGLARDEFGDLGQVFNRALEGFGELQTAGEVQGSLFPGRLAA
                     APGVEAGIVIQAGRQLGGAFAEVVTLSNQQLLLTFGSVGATGVRGALTLAMLKSLLTR
                     RLSDYSLVHAESEQLRVWLLQLLSGNQALLPRFASVLLNPADGTIACNPGGFSLYSGL
                     APFSAAELWESLSTLAPESDTGTSWSLPQGHVLILATSAFATTWREARDLGLLPPLSD
                     FPSTDLTMADWRAHLDRHLKALPHELTQSGEVAVAFLRRN"
BASE COUNT          668 a          908 c         1001 g          555 t
ORIGIN      
        1 gcttggtcag cgttccctgg cggggcagca gcgcatcgag aaacgtggtc gagtcgcaga
       61 cgtgaatccc ctcggcaacg aattccccag ccagggcttt cagcagcgca tcatcgtttc
      121 ggatcggcag tttttcgaaa accttcagca gacgcgtgtc cggttgaagt tcctggaaca
      181 tgcgggtctt cgtgattttg ccggccatga ccagacgatt gacgccctcg cgcaggaaaa
      241 catcgatcat cgactgcagc tgcccgacgt tgaaccagtg cagctgttcg accagcggct
      301 cgagttccgg cgaagtctct tcgcggatcc cgaccgcgat gacctcgttc ccagctttac
      361 gcgcggcatc ggcaaaatac agggggaaac gaccatatcc ggcaatgagt ccgattcgtg
      421 acatgactgg ctccgctccg ttcacgggct gattcgcgca ggcatcatac cacgttattc
      481 caggaatgaa aacccctccg tctcgctcag aaaagccgtt gtccatgccc atctgcggtt
      541 cagaaacgcc tttcggccgg caggatcatg tgaaaaattt cgcgaggagg tgaccgtgtc
      601 gaagagaaac gggtcagttt cgtcgaagaa aggcgaccgc gacctcgccc gactgggtca
      661 gttcgtgcgg gagcgccttc agatgccggt ccaggtgcgc tcgccagtcg gccatggtca
      721 gatccgtgct cggaaagtcc gacagaggcg ggagcaatcc cagatcgcgg gcttctcgcc
      781 aggtggtcgc aaaggcggaa gtggcgagaa tcagcacgtg accctgcggt aggctccagg
      841 aagtgcccgt gtctgattcg ggagcaaggg ttgagaggga ttcccagagc tcggcagcgg
      901 aaaacggcgc gagaccggag tacagagaga aaccgccggg gttgcaggcg atcgtcccat
      961 ctgcggggtt cagaagaaca ctggcaaagc gcggaagcag tgcctggttt ccggaaagca
     1021 gctgaagcag ccacacccgc agctgttccg attcggcatg gaccagcgag taatcggaga
     1081 gacggcgagt cagaagtgat ttgagcatgg ccagggtgag agccccgcgt acaccggtgg
     1141 cgcccaccga cccgaaggtc agcagcagct gctgattcga cagggtgacg acctcggcga
     1201 aagccccgcc gagttgacgc ccggcctgaa tgacgattcc cgcctccact cccggggctg
     1261 ccgccagcct tcccggaaac aggcttccct gaacctctcc ggcggtctgc agttcgccga
     1321 acccttccag ggctcgattg aagacctggc ccagatctcc gaactcgtcg cgggccagac
     1381 cggtcacgcg aaaatcgaac tgccgccgtt cgatggccgt cgtcgccgcg gcaagccgct
     1441 gaatcggcat gaggatgctg cgtgaaagca ccgtggcaat catgatcgtg atcagcaggg
     1501 ccagcagcgc gatggtgagc agcaggcgtt tcaagcgcag catgcgcttc tcgaccacgg
     1561 tctggggagt gagtgccagc aggtggtgac ttgacaggtg ctgcccggga acgccgaccg
     1621 ccagccagga acgaccgttc cagccgatcg cgtccatgac ggtgcgctgc tcgcgacgca
     1681 cgcgctcgct gaaggcgtta agtggtctcg gatcaggcgt gttttccggt acgagttgaa
     1741 aagagtgcgt cgtcacggcg aagaagcggg gggcgcgtcg atcacgcgct gcctgcaggc
     1801 gcgtccggag aatgtgctgt cgctgcagtt tccattgtga ccattgggcg agagccagat
     1861 agcgcagggg gccggccaga ccttccgtca gcggagacaa catgatgact acgcgtttgt
     1921 ccatgttgct aaagagcgaa atacgcccca attgcagaat catggtgtta cgaaactcct
     1981 ctgttgactc gcgacccatg aagtcttcga tcagttcgtt cagtgccaga ttcttgcgcg
     2041 ggttcgcggc tgtacccgtg agatcgcgaa acagagcggc aagaacggcg ctgaacagtc
     2101 gttgttcctt gtcgactccc gatgagagcg gacgaccagt cggtaacagt ttttcctggg
     2161 tcaggatcgg cgtttcgtca tcattgacca ggtagaactc gctggtgttg agctgcgcga
     2221 tgcccgaagc aagaatcggc gatcgggaaa acgtcgtctg aggtctcaag gatgagctga
     2281 cggtggcggt cagcgaggca aacgccgtct gcgtttccag cagggtcgag gaaaaccggc
     2341 tgtcaatctg tcgcagaaac tgctcggaac gcagaaacac cgagttgcgc agatcgcttt
     2401 cccgatgttg cagatactcg tgggcgagaa accccatgcc cgtgaggggg aggctggtcg
     2461 cgaacagaaa ggcggccgaa atctgccagc gaatcgacac gaactgcacc cgctcctgga
     2521 cgaacgcgcg aaaaccccac agactcaagg tcaggaacaa gccgatcaac agaacatgcc
     2581 acacgcgtcg ggtggtgttg ctgcgctcgt gcaggcgtac cagcggctga aacgcaaaaa
     2641 gcactccgtg ctgcggcatg gcgcgacaga cgaccaggta gtgcggtgtg tcgatctccg
     2701 tttcctgcga ttggcgaaac tgagcgatcg cggcttcgag ctcgtggctg aagggagttg
     2761 ccgcgggaac cgccatgccc gagttgtttt ccgattgcca gaatgaggtc cagccgagac
     2821 gaatgccgcc gcgcaggtgc cggttcagat gacgcaacgg aatcggccag tactgacctt
     2881 gccgaaagaa ggactcctcg aattggacca gcatgccggg accgcgctcg ggcatgcgga
     2941 accagatgga gcgggactcg ggaaacgcat tcgtgtgcgc gacgaaaatg ccggggaaca
     3001 gactctcgcg aaggtcccgc aacgtgacca gaggtcctag aagatgttcg gcgagaagct
     3061 gcgcctgcgc gccgaatctg cgcagcgtct gcgtctcgcg atgccgctgc gccgcggcca
     3121 gcagctgtcc ga
//
LOCUS       DAORHD010000503         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35611_length_3121_cov_3.920091, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000503 DAORHD010000000
VERSION     DAORHD010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35611_length_3121_cov_3.920091"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1337
                     /locus_tag="PKO06_11155"
     CDS             372..1337
                     /locus_tag="PKO06_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70246.1"
                     /translation="MSYSQIPGWFVRHEALPPAENMAWDEALSLAVRRGRLGGFLRFY
                     RWNPPTLSFGYFQKPSRILRPEALTCREIGLVRRASGGKMVFHDQEWTFSCALPTSVL
                     KQRQTSTDVDGFLTWFRLLVEPLAITLRQLGVAIEFAEVQKGGRSGARAVDEGTAPRP
                     APAGDERQLLKSSATAPRDRVHCFSAAAGHSLMVGERKLIGAAGIVREGVLLVHGSIP
                     IAPGRELPPLFTSGIDPQAHRETIYLKEVLAANMIERLPERVAATMQEYLGIILNEMS
                     TTPAVVADVNPLVKRLAEEKFADLDWPAQPVERWDELASSLLTQK"
     gene            1435..>3121
                     /locus_tag="PKO06_11160"
     CDS             1435..>3121
                     /locus_tag="PKO06_11160"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HNV70247.1"
                     /translation="MTVSVIQLPRGGTLIKTPDGLLQIGATPETIKDTLTMSGEVPDT
                     FVLPNKLFSSERGVSLADIEFPAYYNFFIKNRPVKLIGQTHQIETIINVLQEAVLGPN
                     DIDVTREFPLGVSRDTMPNLKSEMEFLRARDLSGRRKMEIADIAIGIPWGPNNRVPFG
                     HLALERTPGGTIKVVEGAEVLTEVPLDVSFGDSRFPNERTKPFEPPLFGITVIGSGHG
                     FDPKEMTSGFIVWVNRRGILVDPPVDSTLWLKAMDINPRLIEDCVLTHCHADHDAGTL
                     QKLLEEKKINLYTTETIMGSFIRKYRSLTGLQLKTFMGLFNFQPVTLTVPVRINGAEF
                     RFFYTLHSIPTIGFEVYYGGKSFAYSSDSLNDPPTIKKIYETGVINRYRMIELINFPW
                     HHTVVFHEAGIPPIHTPLKNLGELPESVKEHLYLIHVSQRSIPPESRLKKAMAGPDET
                     IVLPVTPPPTNEALEYLNVLNNIDLFCELPIEKAREFLTLVKVETYQKGDLVIKRGSV
                     GDRFYMIVNGRASILRDGKALKTYTNNDYIGETSMILNVPRTADVFAESDLKML"
BASE COUNT          684 a          859 c          883 g          695 t
ORIGIN      
        1 caatctgccc tgttccgcga atctgaagga agaagaggca gtacgggtcg ccgaggcgat
       61 acgcgagttt caggcgcgca tggcgatctg aggttgagcc gcaaaacgac cggaaaaaaa
      121 ggagccggtg tggtgacacc ggctcaaatt aataaggagg caaacaaatc catacccctt
      181 ttcgtccgaa gttgatgttt ccttaacgtt ttgtgaattg acctggacgg acattggcga
      241 acaaactcgg acaacggatg atgtgaacgc atggatacag cacttccggt acacaaaact
      301 attttttcct tcttcacctc gctcgccatc gccgtatgtc tcgtggcgta ccgcgctgcc
      361 ggaagctgtt gatgagttat tcccagattc ccgggtggtt cgtaaggcac gaggccttgc
      421 cgccggccga gaacatggcc tgggatgaag ccctcagcct ggcggtgcgg cgcggtcgtc
      481 tgggcggctt tctgcgcttt tatcggtgga acccgccgac cctgtcgttt ggctactttc
      541 agaaaccgtc gcggattctt cggcccgaag ccctgacctg tcgggaaatc ggtctggtac
      601 gacgggccag cgggggtaaa atggtctttc acgatcagga atggacgttc tcgtgcgcgt
      661 tgccaaccag cgtgctcaaa caacggcaga catccacgga cgtcgatgga tttctcacct
      721 ggtttcggct gctggtcgaa cctctggcga tcaccctgcg gcagcttggg gtcgcgattg
      781 agtttgccga agtgcagaaa ggcgggcgat cgggcgcacg tgccgtcgac gaaggaactg
      841 cgccgcgacc ggcaccggcc ggagacgaac gtcagctgct caagtcgtcg gcaacagcgc
      901 cacgtgatcg ggtgcattgt ttttcggccg ccgcggggca ctcgttaatg gtcggcgaac
      961 gcaaactcat cggtgccgcc ggtatcgtgc gtgagggggt tctcctggtg catggctcga
     1021 tcccgattgc tcccgggcgg gaactgccgc ctctcttcac ctccggcatc gatccgcagg
     1081 cgcatcgaga gacgatctac ctcaaagaag ttctggccgc gaacatgatc gagagactgc
     1141 ccgaacgggt tgccgcgacg atgcaggaat atttaggaat aattctgaat gagatgtcga
     1201 cgacacccgc agtggtggcg gatgtgaatc cactggtgaa acggttggcc gaagagaagt
     1261 ttgccgatct cgactggccg gcgcaaccgg ttgagcgctg ggacgaactc gcctcgagtc
     1321 tcttgacgca aaaatgatga tttcacgtct tttttccgcg tctattgttt gcatattctc
     1381 agcggtttga ttattctgcc tcagagtgca gaagtttctt gcggtgggga atacatgacg
     1441 gtatcggtta ttcagttgcc acgcggtggc accttgatca agacacctga cggtctgctg
     1501 cagatcggcg cgacgcccga aacgatcaaa gacacgctga ccatgtcggg tgaagttccc
     1561 gataccttcg tgctgccgaa taaactgttc agttccgagc gcggagtctc gctggcggat
     1621 attgagtttc ccgcctatta caactttttc attaaaaacc ggccggtcaa gctgatcgga
     1681 cagacgcatc agatcgaaac cattatcaat gttctgcagg aagctgttct cgggccgaac
     1741 gatatcgacg tgacccgcga attcccgctg ggagtcagcc gcgataccat gcccaatctc
     1801 aagtccgaga tggaatttct gcgcgcccgc gatctgagcg gccgccgcaa aatggaaatc
     1861 gcggatattg cgatcggcat tccgtggggt ccgaacaatc gtgtgccgtt cggtcacctg
     1921 gctctcgaac gcacgccggg cggaacgatc aaagtggtgg aaggcgccga ggtgctgacc
     1981 gaagtgcccc tcgatgtctc gtttggcgat tcgcgctttc cgaacgagcg taccaagcca
     2041 ttcgaacccc ctctgttcgg gattaccgtc atcggcagcg gccatggatt cgacccgaag
     2101 gaaatgacct cagggtttat cgtctgggtc aatcgccgcg gaattctggt cgatccaccc
     2161 gttgactcga ccctgtggct gaaggcgatg gatatcaatc cccgcctgat cgaggactgt
     2221 gttctcaccc attgtcacgc tgatcatgat gccggaactc ttcagaaact gctggaagaa
     2281 aagaaaatca acttgtacac gaccgaaacg atcatgggca gtttcatcag aaagtaccgg
     2341 tcgctgaccg gtttgcagct caagacgttt atggggttgt tcaattttca acccgtcacg
     2401 ctcaccgtac cggttcgcat caacggagcc gagttccggt tcttctatac gctgcattcg
     2461 attcccacga ttggcttcga agtgtattac ggcggcaaga gctttgccta ttcttccgac
     2521 tcgctgaacg acccgccgac catcaagaag atctatgaga cgggtgtcat caaccgctac
     2581 cgcatgatcg agttgatcaa tttcccgtgg catcacaccg tcgtgtttca cgaggctgga
     2641 attccgccga ttcacactcc gctgaaaaac ctgggcgaat tgccggaaag cgtgaaagag
     2701 cacttgtacc tgattcatgt gtcgcagcgc tcgattccac ctgagtctcg actgaagaaa
     2761 gccatggctg gtcctgacga gacgatcgtg ctgccggtga cgcctccccc caccaatgaa
     2821 gcgctcgaat atctcaatgt gctcaacaat atcgatctgt tctgtgaact gccgatcgaa
     2881 aaggcccgcg aatttttgac cctggtgaaa gtggaaacct atcagaaagg cgatctggtc
     2941 atcaaacgcg gcagcgtcgg tgatcggttt tacatgatcg tcaacgggcg cgcctcgatt
     3001 ctgcgcgatg gcaaggccct gaagacctac accaataatg actacatcgg cgaaacatcg
     3061 atgatcctca atgtgccgcg tacggcagat gtcttcgcgg agagcgatct gaagatgctg
     3121 g
//
LOCUS       DAORHD010000504         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35616_length_3121_cov_3.534899, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000504 DAORHD010000000
VERSION     DAORHD010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35616_length_3121_cov_3.534899"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="PKO06_11165"
     CDS             complement(<1..1056)
                     /locus_tag="PKO06_11165"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70248.1"
                     /translation="MINITRLYGDRATTGDPLRYGEDGPKQVGHRHHFHGHGRAPTAA
                     ERRPVTVWNVTKRCNLKCVHCYTDSCTSLAPDELTYEEGERFLEDLAKFQVPAILFSG
                     GEPLTRPDLFELAHKAVTIGLRPVLSTNGVLIDRACAQRLKDLGFIYVGISLDGIGET
                     NDRFRGRTGAFEEALRGFRHCREVGQRVGLRLTLTRRNVLDLDRIFDFILEEGIPRAC
                     FYHLVYSGRGRGLSDEDLTHEESRQAIDLICARTRKAIEAGRDLDILTVDNHVDGPYI
                     YLKLLQEDPERAARMKQMLEWNGGGAWSTGVGIADVDWAGNVHPDQFWQDHSFGSIRQ
                     RPFSQIWLDTSDPLMAGL"
     gene            complement(1073..2908)
                     /gene="glmS"
                     /locus_tag="PKO06_11170"
     CDS             complement(1073..2908)
                     /gene="glmS"
                     /locus_tag="PKO06_11170"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNV70249.1"
                     /translation="MCGIIGYVGDKPANEIIFEGLTRLEYRGYDSAGIAVNENGKLLV
                     KKDVGKLRHLQKSLLDEFGSTSGTGIGHTRWATHGRPSTFNSHPHGDCKGTIQVAHNG
                     IIENYIPLKQRLMAAGHTFASETDSEVLAHLVESHYRGNLAEAVRAALREVEGAYAVV
                     VIHEKHPGELICYRKESPLIIGLGRKENFVASDVPAILRHTREVVYLKNFEGAVVRRD
                     GVDFFDLEGNPLEKQAIHVDWNPIAAEKGGFSHFMLKEIFEQPQVIRNTIGGRTAEED
                     GKIYLNELGLSGPDLFKVQRIVMVACGTAYYSGCVGKYLIEKLVRIPVECDLASEFRY
                     REPLVDANTLVIAISQSGETADTLAAVREAKARGAKVLGIVNAKDSSLTREVDGLVYI
                     HAGPEIGVASTKAYIAMLTAQVLMALLFGKIRGTLTPKFVTEIIGELKRIPQDIEKVL
                     AQAESIRELAKRFQHYRDFLYLGRGINYPTAMEGALKLKEISYVHAEAYAAGEMKHGP
                     IALIDKDMPVFAIATASTLNEKILSNLKEVQARSGVLIALATEGEEDHLDKFVEHVIR
                     LPRCSEILSPLLNVVPLQLFAYYVADLRGFDVDQPRNLAKSVTVE"
BASE COUNT          644 a          941 c          879 g          657 t
ORIGIN      
        1 caaccccgcc atcagcgggt cagacgtgtc gagccagatc tgggaaaatg gtcgctgacg
       61 aatgctgcca aagctgtgat cctgccagaa ctggtcgggg tgaacgtttc cggcccagtc
      121 cacatcggca atccccactc cggtcgacca ggcgccgcca ccgttccatt cgagcatctg
      181 tttcatgcgc gcggcacgtt ccgggtcttc ctgcaggagt ttcagataga tgtacgggcc
      241 atcgacatga ttgtcgacgg tcagaatatc gagatcgcgg ccagcctcga tggccttccg
      301 ggtgcgggcg cagatcagat cgatcgcctg acggctttct tcatgcgtca gatcttcgtc
      361 tgataaccct cgaccacgac cggaataaac gagatgatag aagcacgccc gcggaattcc
      421 ttcctcgaga atgaaatcaa agatgcgatc aagatcgagc acattgcggc gtgtcagcgt
      481 caggcgaaga ccgacccgct gtccgacctc gcgacaatgc cgaaagcctc gcagggcttc
      541 ttcgaaagcc cccgtacgtc cacgaaagcg atcgttcgtc tcaccgatcc catcgagcga
      601 gatgccaacg tagatgaaac cgagatcttt cagacgctgg gcacacgcgc gatcgatcag
      661 aacgccattg gtcgacagta ctggtcgcaa tccgatagta actgctttgt gagccagttc
      721 aaacaagtct ggtctggtga gcggctcgcc ccccgaaaag agaatcgccg gaacctggaa
      781 cttcgccagg tcttcaagaa accgctctcc ttcttcgtag gtcagttcat cgggggcaag
      841 actggtacaa ctatcggtat aacagtgaac acacttgaga ttacagcgtt tcgtcacgtt
      901 ccagaccgtg acgggtcgac gctcggcggc cgtcggcgcg cgaccgtgac cgtgaaagtg
      961 atgacggtga ccgacctgtt tcggcccgtc ttcgccgtac cgcagcggat cgccggtggt
     1021 ggcgcggtcg ccatacagtc gcgtgatatt gatcatgaac tggcgcagcg cctcattcga
     1081 cggtcacgga tttggccaga ttacggggct gatcgacatc gaagccacgc agatcggcga
     1141 cgtagtaagc gaacaactgc aacggcacca cgttcaacaa cggtgacagg atctcggaac
     1201 accggggcag gcgaatcaca tgctcaacga atttgtcgag atgatcctct tcgccttccg
     1261 tggccagggc gatcagaacg cccgaacgag cttgtacttc cttgaggttc gagagaatct
     1321 tctcgttcaa ggtcgaggca gtcgcgatgg caaacaccgg catatccttg tcgatcagcg
     1381 cgatggggcc gtgcttcatc tcaccggccg catacgcttc ggcatgcacg taactgattt
     1441 ccttgagttt gagcgctcct tccatggccg tcggatagtt gatgccccga cccaggtaca
     1501 ggaaatcacg ataatgctga aagcgcttcg ccagttcgcg aatcgattcg gcctgtgcca
     1561 gaaccttttc gatatcctgc ggaatacgct tgagttcgcc gatgatctcg gtcacgaact
     1621 tgggcgtgag cgtaccgcga atcttgccga acaacagcgc catcagcacc tgcgccgtca
     1681 gcatggcaat gtaggccttc gtcgaagcga cgccgatctc aggcccggca tggatgtaaa
     1741 ccagaccatc gacctcgcgg gtcaacgacg aatccttcgc attcacaatg ccgagaacct
     1801 tggctccgcg tgccttggct tcgcgcacgg ccgcgagagt atcggcggtt tcacccgact
     1861 ggctgatcgc gatcaccagc gtgttcgcgt cgacgagcgg ttcgcgatac cgaaactcgc
     1921 tggccagatc gcactcaacc gggatacgga ccagcttctc gatcagatac ttgccgacgc
     1981 atcccgagta atacgcggta ccgcacgcta ccatgacaat gcgctgcacc ttgaagagat
     2041 ccgggcccga aagtccgagt tcgttcaggt aaatcttgcc gtcttcttca gcggttcgac
     2101 caccgatggt gttacgaatg acctgcggct gctcgaagat ctctttgagc atgaagtgcg
     2161 agaacccgcc cttctcggcc gcaatcgggt tccagtcgac atgaatcgcc tgcttttcga
     2221 gcgggtttcc ttcgagatcg aagaagtcga ccccatcgcg ccgcaccacg gcaccctcga
     2281 agttcttcag gtagacgacc tctcgcgtgt gccgcaagat cgccggcaca tcagaggcaa
     2341 cgaaattttc cttccggccc aggccgatga tcagcgggct ctctttgcga tagcagatca
     2401 gttcacccgg atgcttctcg tgaatgacga cgaccgcata ggccccttcg acctcacgca
     2461 gagcggcacg cacggcttca gccagatttc cgcggtagtg tgattccacc agatgcgcca
     2521 gcacttccga atccgtttcc gaggcgaacg tatgccccgc cgccatcagc cgctgtttca
     2581 ggggaatgta gttttcgatg ataccattgt gagcgacctg aatggtgccc ttgcagtctc
     2641 cgtgcgggtg cgagttgaag gtcgaaggtc ggccgtgtgt tgcccagcgt gtgtggccga
     2701 tcccggtccc ggaggtcgat ccgaactcgt cgagcagcga tttctgcaga tggcgcagtt
     2761 tgccgacatc ctttttgacc agcagcttgc cgttctcgtt cacggcaata ccggctgaat
     2821 catagcctcg gtattcgagt cgggtcaggc cttcgaaaat gatctcattc gccggcttgt
     2881 cgccgacgta accgatgatt ccgcacatgg agctacctca ggttctttga attgttcgcg
     2941 tggtggcgtc agtatttgac tttgcccggt gccggcttcg gctcggaagg cggcgccggc
     3001 gctgcttcag gctctgccgg caggcgcgac ttgacactcg ccagacggtt ctcggcctgc
     3061 acggcgaacg acgtgccggg atatttctcg ggtcttcagc acggtgcggg gtaccaccac
     3121 a
//
LOCUS       DAORHD010000505         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35688_length_3118_cov_2.852432, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000505 DAORHD010000000
VERSION     DAORHD010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35688_length_3118_cov_2.852432"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKO06_11175"
     CDS             <1..832
                     /locus_tag="PKO06_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNV70250.1"
                     /translation="EVVERLRWMENVLAPVVKDALAVRGEINLKSLIAQALMMGDEVH
                     NRNRAATSLLFRELAPSILETKHANPDKKKVLEFINSNDHFFLNLSMPAAKAILQSAE
                     WIEGSSLVTTMCRNGTDFGIRIAGLKDRWFTGPAQIVKGLYFPGYTEDDANPDIGDSA
                     ITETVGLGGFSMAAAPAIVKFVGGTVQDSLHYTQRMYEITEAESSSFQIPQLDFRGSP
                     TGINLLKVCELGILPQINTGVAHKEPGVGQVGAGLVNPPMKCFEDALEAFVEKYCAND
                     "
     gene            912..1706
                     /locus_tag="PKO06_11180"
     CDS             912..1706
                     /locus_tag="PKO06_11180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNV70251.1"
                     /translation="MIPKFKMYDLTQRLSIHTPPWPSYMPLQVQYFKRIAGAHMGQGA
                     NGQIIKTSNHVGTHIDGEIHFVANGRTIGDTPLDEFCGEGVVVDISKDVEDYSLYTPE
                     MLTKRADIRKGDILIINTGYHRYGWDQKEADEVRYMVKHPGPSPEFHKWALSMKFKWI
                     GVDCGSADHPMNTILRQWHPKLFQEAEARLKKKYKKSWDEFFPYEEYYQVMHLKLFPK
                     GLIHAENLGGDIDKLNNKRAWIGLFPWRAIELESCISRIVAFEFGK"
     gene            complement(1786..1926)
                     /locus_tag="PKO06_11185"
     CDS             complement(1786..1926)
                     /locus_tag="PKO06_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70252.1"
                     /translation="MSHHHTPENTPVRTQEGTDQTLIAIVWKTALTIVISGIFAKVIG
                     VF"
     gene            complement(1997..2368)
                     /locus_tag="PKO06_11190"
     CDS             complement(1997..2368)
                     /locus_tag="PKO06_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70253.1"
                     /translation="MMRMLLVVWLCLGNLVSSVTPDSALEQRLLRGQSQAVCRIQSEP
                     QGRIAEATAEPQSRLSLMPRVYRLIAGEMAFTRLVLPHHDTSQCLRLASSKLRRQRSI
                     FAQFQPHDWGLCTTRLPRADG"
     gene            complement(2513..>3118)
                     /locus_tag="PKO06_11195"
     CDS             complement(2513..>3118)
                     /locus_tag="PKO06_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70254.1"
                     /translation="EPVQTEPVAEPVVAAPAPVVPVQSEPAQPEPVMIETPVAVEPPV
                     AVTAPVEPEPVIPVPVTPTSVVAAEPEPVTAPVTENVVEKQEVSSSRDAFMTLLEKMQ
                     SRRDWRREQARRHAVTLPSERTPAVKIVGDQSSAVESTLSVAEKLKLHLERLSARREL
                     REREAAKYGLQLPSHRTPDSGIVISFSPTGGPAAAYAPGAR"
BASE COUNT          726 a          908 c          892 g          592 t
ORIGIN      
        1 cgaagtcgtc gagcgtctgc gctggatgga aaatgtcctg gctcccgtgg tcaaagacgc
       61 gctggccgtg cgcggcgaaa tcaatctgaa aagcctcatt gctcaagcgt tgatgatggg
      121 cgatgaagta cacaaccgca accgggccgc gacttctcta ttgtttcgtg aacttgcccc
      181 ctccatcctc gagacgaagc atgcgaaccc ggacaaaaag aaagtactcg agttcatcaa
      241 cagtaacgac cacttcttcc tgaacctgtc gatgccggcg gccaaggcca ttctgcagtc
      301 agccgaatgg atcgagggtt cgagcctcgt caccacgatg tgccgcaatg gcacggactt
      361 cggcattcgc attgccggtc tgaaagaccg ctggttcacc ggtccggcac agatcgtgaa
      421 ggggttgtac tttcccgggt atactgaaga cgacgccaac cctgatatcg gcgacagcgc
      481 gatcaccgag acggtgggtc tcggcggttt ttcgatggcc gcggcgccgg cgatcgtcaa
      541 gttcgtcggc ggcacggtac aggattcgct gcattacacg caacgcatgt atgagatcac
      601 cgaagccgaa tcgtccagtt ttcagattcc tcaactcgac ttccggggct cgccgacagg
      661 catcaatctc ctgaaggtgt gcgaactggg cattctgcct cagatcaata ccggggtcgc
      721 ccacaaggag ccgggcgtcg gacaggtcgg agccggtctg gttaacccgc ccatgaagtg
      781 ttttgaagac gctcttgagg cctttgtcga gaaatactgc gccaacgact gaggaaatgg
      841 agctgtctct tgcgcaaacg cccgagatat ggttgtattg atatcaaatt cctatcaagg
      901 agattttcgg aatgattcct aagttcaaaa tgtacgatct gacccagcgc ctgagcattc
      961 acaccccgcc atggcccagc tacatgccac tccaggtaca gtatttcaag cgcatcgccg
     1021 gagcccacat ggggcagggt gccaacggtc agatcatcaa aaccagcaat cacgtcggca
     1081 cacacatcga cggcgagatt cacttcgtcg ccaacggccg caccatcggc gatactccgc
     1141 tcgatgaatt ctgcggtgaa ggcgtcgtcg tcgacatcag caaggatgtc gaggattaca
     1201 gtctctatac gcccgaaatg ctgaccaagc gcgccgacat tcgcaagggt gacattctga
     1261 tcatcaacac tggctaccac cgctacggct gggatcaaaa ggaagccgac gaagtgcgct
     1321 acatggtgaa acaccccggc cccagccccg agttccacaa gtgggctctc tccatgaagt
     1381 tcaaatggat cggcgtggac tgcggatcag ccgaccaccc gatgaacacc attctgcgcc
     1441 agtggcaccc gaagctgttt caggaagctg aagcccgcct gaagaagaag tacaagaagt
     1501 cctgggacga attcttcccc tatgaagagt actaccaggt catgcatctc aagctcttcc
     1561 cgaaaggctt gatccatgcc gaaaatctcg gcggagacat cgacaaactg aacaacaaac
     1621 gtgcctggat cggcctgttc ccctggcgcg ccatcgaact cgaaagctgc atcagtcgca
     1681 tcgtcgcctt cgaattcggc aagtaacccc gcgttcgaac cagaaacagt gagcccaccc
     1741 cttcgggtgg gctctttgtt tctccgttga aaccgaaacg ccgacttaga agacgccgat
     1801 caccttggcg aagatgccac tgatgacgat cgtcagagcc gtcttccaga cgatggcgat
     1861 gagcgtctgg tcggtacctt cttgagtgcg gaccggggtg ttttcaggcg tgtgatgatg
     1921 tgacatgata tagatatcct ttcgtttttt cagaaatggc aaaactcaaa acgtgactga
     1981 agaaaacgaa gggttcctat ccgtcggcgc gcggcaaccg cgtggtacac aaaccccagt
     2041 catggggctg aaactgagcg aaaatggaac gctgccgccg cagctttgag gaagccaggc
     2101 gcaggcactg cgaggtatcg tgatggggca aaaccaagcg cgtaaaggcc atttcacctg
     2161 cgatcaaccg gtacactcgc ggcatgagag acaaccgaga ctgcggttcc gcggtcgcct
     2221 cagcaattct gccctgcggc tcactctgaa tgcggcagac agcctgtgac tgcccacgca
     2281 gcaagcgctg ctcgagagct gaatccgggg tgacactcga aacgagattc cccaggcaga
     2341 gccagacaac cagcaacatc cgcatcatgg actcagtata gcagacggag cgttcgcgcg
     2401 gagagacaaa ttcaggaaaa gaactacagg atgcctgcaa aacgggcatc tgaaggtctc
     2461 gatgaggcca ccacgacgca tacgaacgtc cagacggacg cccaaagaat cgtcagcgtg
     2521 ctcccggcgc gtaagccgcg gcagggccgc cggtcggcga aaagctgatg acgataccgg
     2581 aatcaggggt gcgatgactg ggcaactgaa ggccatattt tgcggcttcg cgctcgcgca
     2641 gctcgcggcg agccgagagg cgttcgagat gcagtttcag cttctcggca acggacaacg
     2701 tcgattccac ggcggatgac tggtcgccca cgatcttgac ggctggggtt cgctcgctgg
     2761 gcagcgtcac ggcatgcctg cgcgcctgct cacgacgcca gtcgcgacgt gactgcatct
     2821 tttccagcaa ggtcataaac gcatcccgcg aggaagagac ttcttgtttc tcgacgacgt
     2881 tctcggtgac aggggcggtc actggctcgg gttcggcagc gacaacgctt gtcggcgtga
     2941 ccggaactgg tatgacaggc tctggctcga ccggggccgt cacggcgacc ggaggctcaa
     3001 ccgccactgg agtttcgatc atgactggct caggctgagc aggttcgctc tgcacgggaa
     3061 ccaccggcgc cggggccgcg acaacgggct ccgcaacggg ttcggtctga acgggttc
//
LOCUS       DAORHD010000506         3117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35709_length_3117_cov_3.468321, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000506 DAORHD010000000
VERSION     DAORHD010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35709_length_3117_cov_3.468321"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2785)
                     /locus_tag="PKO06_11200"
     CDS             complement(<1..2785)
                     /locus_tag="PKO06_11200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV70255.1"
                     /translation="MTATSHHAIPDSVFRKVLMAVLLAILFIWLPGGALVFTIHHLQT
                     EYDSDRRSHAERQLESFLERAADEDDAVRFWSRVCQRIDQQAMLATDSLSQLSFEFAR
                     LQRWIPDASFRLIVWNRKGSIIREDPVTTMNTADWQTIASDLNLIQAPVRERNTYEAV
                     QRLQGSWQRLFGGGLVAQRVLNYTRNRRLLINPIGLKESARIWPGISREGAYILYLDA
                     AAFRGPVGLRHACQFPPRWRYRGLAVGFLDPQEPDTIFASMPEFRQALAAIGGIRGFH
                     QQATHAIGWFVAYRSLPSGRILTAGIRQPFLTVHGPIILGLFSLLLGLVSVRGTARLI
                     RVATATSGHGFSVQAKLAVFFLLANGLPLLLLLMMSATQLRNREVSLLSNLHSSCAEM
                     IRHFDEGVRAWNERTRLDVLRNLQKLEHTLATRPFSATEYKQLYRDMARLGCQNFMLC
                     ASSTADWITSNGYYPERGRSLRTGRAFDAKRERFFLEGIQRIIALVNGLPTKPVDRFE
                     LTIEGMFGISAGEFANNLLSGISRLTIWGDGSNRLPMVVKLFRSPDGATVHYLFLATF
                     HPNFIGFRYFQSQLHTLNRNHLGLRFLVRSLDPSVINSISHPDRARQPVSRALTWVQF
                     PREIHLPRALVNHVQSLSPLVTVSPEPFLIKNRPYLVIGGAGRELPEFVYFALYPYDN
                     LQKQHREMRRNLLGFGILSLLFSAILAHALGRRFLEPIAVLQEGTRAIRRHAFTCRLP
                     DLGNDEFGRIGKAVNNAMQSLEELAVARAVQENLLPEGVFVTGSCRGFGRTIPMSELG
                     GDFFDFQRTGEFTSVLSIGDVTGHGVPAALVMAMAKSVLYRLHQDGISSECIYSQLNE
                     LLLQMHRRKTRRALSLQSVEIDDRSEVLRITSAGHCYPAHLTPAGVSLIRFSSTPVGV
                     TQRHRFATQEVVFR"
     gene            complement(2782..>3117)
                     /locus_tag="PKO06_11205"
     CDS             complement(2782..>3117)
                     /locus_tag="PKO06_11205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV70256.1"
                     /translation="RIAFFLDQFLHLAGRALGVSKTSGIAETQITLAPGEQLILYTDG
                     FVEALSHDGSLVGYERCLDLFQQCRHDDPQAFVRGFVEAHHQLAPSLQDDSTLVIVSR
                     RSATAMEET"
BASE COUNT          719 a          865 c          944 g          589 t
ORIGIN      
        1 gccggaagac aacctcctgc gtggcaaaac ggtgccgctg agtgactccc acgggtgttg
       61 aggagaaccg aatcagggag acaccggcag gggtgagatg agcaggatag caatgtcccg
      121 cgctggtaat acgcagcact tctgaacggt catcgatctc cacgctctgc agactcagcg
      181 ctcgccgggt tttccgacgg tgcatctgca gaagcaactc attcaattgt gaatagatac
      241 actcagagct gattccgtct tggtgtaacc ggtacaagac cgacttggcc atggccatga
      301 cgagagcggc cggcacccca tgaccggtca cgtcaccgat agacaacacg ctggtgaatt
      361 cgcccgtgcg ctggaagtcg aaaaaatccc cccccagctc cgacatcgga atagtacgac
      421 cgaacccgcg acaggaacca gtgacgaaga cgccctcagg caagaggttt tcctgaaccg
      481 cgcgagccac cgccagttcc tccaggcttt gcatggcgtt gttgacggcc ttgccaatgc
      541 gtccgaactc atcgtttccc agatcgggca ggcgacacgt aaacgcatgc cgccggatag
      601 cgcgagtccc ctcctgcaaa acggcaatcg gttcgagaaa gcgcctgccc agagcgtgcg
      661 ccagaatggc gctgaacagc agactcagga ttccgaaccc gagaaggttc cgccgcatct
      721 cccggtgctg cttctgcagg ttgtcatacg gatacagggc gaaatagacg aactcgggca
      781 attctctgcc cgcaccgcca atgaccagat agggccgatt cttgatgaga aaaggctcag
      841 gcgaaacggt caccaggggg gaaagagatt gcacgtgatt caccagagct cgaggaagat
      901 ggatttcgcg cgggaactgt acccacgtga gcgctcggga cacgggttgg cgtgctcggt
      961 caggatgcga gatcgagttg atcaccgaag gatccaggga gcgaaccagg aatcgcagac
     1021 ccagatgatt gcgattcagc gtgtgcagtt gtgactgaaa gtagcgaaaa ccaatgaagt
     1081 tcggatgaaa ggtcgcgagg aaaaggtagt gcaccgttgc cccatcgggt gagcggaaca
     1141 gcttgacgac cattggcaac cgattggaac cgtctcccca gatggtcagc cgcgatatgc
     1201 cggacaacag gttgttggca aactcgcctg ctgatattcc gaacatgcct tcgatcgtca
     1261 gttcaaaacg atcgaccggt ttggtcggca ggccgttgac cagagcgata atgcgctgga
     1321 ttccctccag gaaaaatctt tcgcgcttgg cgtcaaaagc tcgacctgtt ctgagtgagc
     1381 ggccgcgttc gggatagtag ccattcgaag tgatccagtc tgccgtcgaa ctggcgcaga
     1441 gcatgaagtt ctgacatccc agcctggcca tgtctcgata cagttgcttg tattctgttg
     1501 ctgaaaatgg gcgtgtcgcc agcgtgtgct cgagtttctg caggtttcgc agcacatcga
     1561 gccgtgttcg ctcgttccag gctcgcacac cttcgtcgaa gtggcgaatc atctcggcgc
     1621 atgaggagtg cagattactc agcagactga cttcacggtt gcgcagttgc gttgcgctca
     1681 tcatgagtag caacaacaag ggcaggccgt tggccagcag gaaaaacacc gcgagtttgg
     1741 cctggacgga gaacccgtgt ccggaggtcg cggtggccac acgaatcagg cgcgcggttc
     1801 ctcgtacgga gaccagaccg agcaggaggc tgaaaagtcc gagaatgatc ggaccgtgaa
     1861 cggtcagaaa cggctggcgg attccggccg tcaggatcct gccggatgga agggaacgat
     1921 acgccacaaa ccatccgatt gcgtgagtcg cctgctgatg aaatcccctg attccgccaa
     1981 tcgcagcaag ggcctggcgg aattccggca tgctggcgaa gatggtatcg ggttcctggg
     2041 ggtcaagaaa tccgactgcc agaccgcgat agcgccatct tggcggaaac tgacacgcat
     2101 gtctcagtcc caccggtccg cgaaaggccg cggcgtcaag atacagaatg taagcgcctt
     2161 cgcgggagat gcctggccag atgcgtgcgc tttccttgag accgatcggg ttgatgagca
     2221 ggcggcggtt tctggtgtag ttgaggacgc gctgggcgac cagaccacca ccgaaaagtc
     2281 gttgccatga tccttgcagg cgctgaacag cctcataggt atttcgctca cgaaccggtg
     2341 cctgaatcag gttcagatca ctcgcgatcg tctgccagtc agccgtgttc atggtcgtga
     2401 ccggatcctc gcgaatgatg ctgcccttcc ggttccagac gatcagacga aacgaagcat
     2461 ctggtatcca gcgctgcagg cgagcgaact cgaaactcaa ctgggacaaa ctgtcggtgg
     2521 cgagcattgc ctgctggtcg attctctggc agacgcgact ccagaaacgt accgcatcgt
     2581 cctcgtcggc ggcccgttcc agaaaacttt ccagttgccg ttcggcatga gaacgccggt
     2641 ccgagtcata ctccgtttgc agatggtgga tggtgaatac cagagcaccg ccaggcagcc
     2701 agatgaagag gatcgccagg agaactgcca tcaggacttt gcggaagacg gagtccggaa
     2761 tcgcgtggtg agacgtagcg gtcatgtctc ctccattgcc gtcgcagaac ggcgggatac
     2821 gatgaccagg gtcgagtcat cctgcaatga cggtgcgagc tgatggtgcg cttcgacaaa
     2881 gccgcggaca aacgcttgtg gatcatcgtg tcggcactgt tgaaacaggt cgagacagcg
     2941 ctcgtaaccg accagactgc catcatgcga gagagcctcg acgaacccgt cggtatacag
     3001 gatcagctgc tctccgggcg cgagcgtgat ctgcgtctct gcgatacctg acgttttcga
     3061 gacaccgaga gcgcggccgg caaggtgaag gaattgatcg aggaagaagg caatccg
//
LOCUS       DAORHD010000507         3116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35724_length_3116_cov_2.975825, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000507 DAORHD010000000
VERSION     DAORHD010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35724_length_3116_cov_2.975825"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2144)
                     /locus_tag="PKO06_11210"
     CDS             complement(<1..2144)
                     /locus_tag="PKO06_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70257.1"
                     /translation="MSANSQHQPVVSPTQRTHRFRHVLIILGFALLAGLMVTTGFASP
                     SREERILSYLQFCSEVREAVIDGLPCPHSPSQVAAELASDPAGLASWVRSVISYEPTI
                     GSNRGAAGTLAAMAGGDWDRALLLGEMLQAGGYSPRYVQVARTASETDALLASWLNAE
                     SRWRLLTEKTPVDASTADTFDEILTPYGLTKAVLRAQQASQTQVWSAHLSEALSPAES
                     TATVIETAISKIGPASEAGYQALIADVRRAMQYRVLVEIDQDEVPLLFSAGPEGALPA
                     VSACKDLSRLEGAPEELCARLSLAVTMPTSEHETKPASVTVFTWEKNLADLWQQPIRL
                     EIVPVNVSFAEPPQTWAPAQWFERLSGFEQFQVVLRHGDEPLAGPVFTRDSTIQASSQ
                     AGRDEAAGNLGSGLGDLFGGTAEDASEPGSITPSDALSPAPVNSHSAPPLASTADAPA
                     SMTRVASGPARPLVLTLELHRPGRPVLRQQRLLIGALVPDRFPVYTADILAVGGPVGP
                     LTAGWMALDAVTANAPIVGEVLGSNDPQRFEGRDGLKRFPGLLHEWQLGRLAIATRLL
                     RNDPGLLLKSEPTVVMLSNQLRPEPTRKAVGMRVAIDVISDGTTLFPRQPEKQAAAFA
                     ANLRLGIAATRLETVLLSRYLPPPGPQGTFEPWAESLRDGRSPIAVRLPVGGDSSPVC
                     PHPLAAWGMKAEESGRLLVFPGVVAPRVWWSI"
     gene            complement(2156..>3116)
                     /locus_tag="PKO06_11215"
     CDS             complement(2156..>3116)
                     /locus_tag="PKO06_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70258.1"
                     /translation="GMSLNLIGDQRDLPPTDPVKPLAKTIVSGTKNGLTFEVTGWEDR
                     PATSSGVSNSDTFTMRVIELAISSKEATHLLAADSAIRLHLPTSCGDLAVPLSPLTRS
                     IPYGWRGSAQLVPNEKSRVRLVFLLPTAIAGRISGTVSWRTSDLDALLPLGDKAPDHA
                     PPAHTARGNGFECRINRVFHKAEEPNPLYVDVTVKRNLDGTVGVFEPGLLAKIEGESI
                     ELSAQSGNRLFSFGPTRHLEPGEEVRGLYIFEAEGDKVSLVSDLLRLDWPTPVTLLPA
                     DEEWLTHDRPEYPEEKSPDLEDGRIRSLARQAAGTKQQPPPQK"
BASE COUNT          583 a          910 c         1025 g          598 t
ORIGIN      
        1 tcgatgctcc accacactcg aggggctaca acgccgggga agaccagcag tcgaccgctt
       61 tcttcagctt tcatgcccca ggccgccaga gggtgggggc atacgggcga cgagtcgccg
      121 ccgacgggca ggcgtaccgc gattggagat ctcccgtcgc gaagactttc cgcccagggt
      181 tcgaaggttc cctgaggccc agggggcggc agataccggc tcagcaggac ggtctcgagg
      241 cgtgtcgcgg cgattcccag gcgcaggttg gccgcaaagg ccgccgcctg cttttcgggt
      301 tgtcgcggaa acaacgttgt tccgtccgag atcacgtcga tcgccacccg catgccaacg
      361 gcctttcggg tcggttcggg acgaagctgg ttggagagca tcactaccgt cggttcagat
      421 ttgaggagca ggcccggatc gttccgcaga agtcgggtgg cgatggccag gcgtcccagt
      481 tgccattcgt gcagaaggcc tggaaatcgc ttgaggccgt cacgtccttc gaaacgttgg
      541 gggtcgttcg aacccagcac ttcaccgacg atgggggcgt tggcggttac cgcgtcgagg
      601 gccatccagc ctgcggtcag aggaccgact ggtccgccga ccgcgaggat gtcggcggtg
      661 tagaccggga atcgatcggg tacgagtgcg ccgatcaaca gtcgctgttg acgcaagacc
      721 gggcgaccag gtcggtggag ttccagtgtc agcacgagcg gtcgcgccgg ccccgatgca
      781 acacgggtca tcgacgcagg cgcgtcggcc gtcgaggcca gagggggcgc ggaatgtgag
      841 ttcacgggcg ctggcgacaa cgcgtctgac ggtgtgatac tcccaggctc gctggcatcc
      901 tccgcagtgc cgccgaacag gtcgcccaga cctgaaccga gattgcctgc ggcttcatct
      961 ctgccggcct gactgctggc ctgtatcgtg ctgtcacggg tgaacaccgg cccggccagc
     1021 ggctcatcgc catggcggag cacaacctga aactgctcaa acccgctgag tcgttcgaac
     1081 cactgggcgg gggcccaggt ctgaggcggt tctgcaaatg agacgttgac ggggacaatt
     1141 tcgaggcgaa tgggctgttg ccagagatct gccagattct tttcccaggt gaacaccgtc
     1201 acagatgctg gtttggtctc gtgttcggac gtgggcatgg tcactgccag actgagccgg
     1261 gcgcacagtt cctccggagc tccttccagg cgagagaggt ctttgcaggc cgataccgcc
     1321 ggcagggccc cttcgggtcc ggcggaaaac agcagaggaa cttcatcctg atcgatctca
     1381 accaggacac gatactgcat ggctcgtcgc acgtcggcga tcagcgcttg atacccggcc
     1441 tcagaggccg gcccgatctt cgatattgct gtttcaatca ctgttgcggt cgattcggcc
     1501 ggggacagtg cctctgacag gtgagcgctc cagacctgcg tctgactggc ctgctgagct
     1561 cgcagaacgg ccttcgtcag gccatacggc gtgagaattt cgtcgaaggt gtcggcagtc
     1621 gaggcatcga cgggtgtttt ctcggtcaga agccgccagc gactttcggc attcagccag
     1681 cttgccagaa gcgcgtcagt ctcgctggcg gtgcgggcaa cctgaacata cctcggagaa
     1741 taaccaccgg cctgcagcat ttcgccgaga agcaaggcgc ggtcccagtc gcccccggcc
     1801 atggcggcga gggttccagc cgccccgcga ttactgccga tcgtcggttc atacgagatg
     1861 accgatcgta cccacgaggc caagccggcc gggtcggagg ccagttcggc ggccacctgt
     1921 gaaggggagt gcggacacgg aagcccgtcg atgacggcct ctcgcacctc actgcagaat
     1981 tgcaggtagg aaagaatccg ctcttcgcga cttggggagg cgaagcctgt ggtgaccatc
     2041 aagcccgcaa gaagcgcgaa gccaaggatg atcaggacgt gccggaatcg gtgagttctc
     2101 tgcgtcggag aaacaacggg ttgatgttgg ctgttggcag acatgcgtgt actcctcact
     2161 tctggggggg cggctgttgc tttgtaccag ccgcctgtcg ggcgagtgaa cggattcgcc
     2221 catcttcaag gtcgggagat ttctcttcgg ggtactcagg tcgatcgtga gtcagccact
     2281 cctcgtcggc cggcagaaga gtcaccggtg tcggccaatc aagacgcaga aggtcggaaa
     2341 cgagactgac cttgtcaccc tcggcttcga aaatgtacag gccgcgaact tcttcgccgg
     2401 gttccagatg ccgtgtcggg ccgaagctga agaggcggtt gccgctctga gctgacagtt
     2461 cgatactctc tccctcaatc ttcgccagta aaccgggctc gaacacgccg acggttccgt
     2521 ccaggttgcg cttgaccgtg acatcgacat agagggggtt tggctcttct gctttgtgaa
     2581 agacacggtt gatgcggcac tcgaagccat tcccccgggc ggtgtgcgcg ggcggtgcgt
     2641 ggtcgggggc cttgtctccg agcgggagga gcgcgtcgag gtctgatgtt cgccaggaga
     2701 cggttccgga aattcggccg gcgatcgccg tcggcaatag aaagaccagt cgcactcggc
     2761 ttttttcatt cggaacgagc tgcgcggagc cgcgccatcc gtagggaatc gatcgagtga
     2821 gcggagagag cggtacggcg agatcaccgc aagatgtcgg caggtgcagt ctgatcgcgg
     2881 agtctgcagc gagcagatgc gtggcctctt ttgaagaaat tgcgagttca atcactcgca
     2941 tggtgaaagt gtccgagtta gataccccag aactcgttgc tgggcggtct tcccaaccag
     3001 taacttcaaa cgtgagcccg tttttcgtac ctgaaacaat cgttttggcc agtggtttca
     3061 ccggatcagt cggcggcagg tcacgctggt caccgatcag gttcagggac attccg
//
LOCUS       DAORHD010000508         3109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35829_length_3109_cov_3.754748, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000508 DAORHD010000000
VERSION     DAORHD010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35829_length_3109_cov_3.754748"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1765)
                     /gene="recN"
                     /locus_tag="PKO06_11220"
     CDS             complement(74..1765)
                     /gene="recN"
                     /locus_tag="PKO06_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNV70259.1"
                     /translation="MICELHLENVLFIRKALLEFGAGLNVITGETGAGKSVLLESIKL
                     LLGKKGRSGLVLPGRGQAKVQAVFDVKDHRELKTAIDELGLSDSDNPDRLVIARTFKT
                     EGSDKVLVNGILASAQSLRQLGVHLMEIHGQNEHQTLLENRVQRRLLDRTGGDAHARV
                     LSEYTSLYVRRRELAENLRVTEERLGRGTARIEELERMLKDLEALGLNSPEEEQELKE
                     EANRLMHVESIASGVNTARDALSGAEELDGIVKLLRTARDGLKRASEHDVGAKEFLQR
                     VESLEIEAADMERELDRFASRLVFDPERLSFLQSRLADIGRLQRRYGRDCQGLFALRQ
                     EALQEIGELTAPDTALEKIRQELGEVEKLCSACCRKLSERRTKLAEKLSKQVTAELEG
                     LGFPRARVQVALQECEPGPEGAETVEFQVSFNPGAPAGPLKKIASGGELSRVALALKK
                     VLARDDELPTLIFDEVDTGIGGTTAEKVAKSLKALGKEKQVLLVTHLHQIAKEGETHF
                     VVGKQVRGDETEIEIVQVTGKKRETEIARMLGAVGREGRAFARSILQERAQGEEK"
     gene            complement(1762..>3109)
                     /locus_tag="PKO06_11225"
     CDS             complement(1762..>3109)
                     /locus_tag="PKO06_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV70260.1"
                     /translation="LRVYRGAIELVPFGGRMLVVNLIGTEEYLRSVVPCEVGKDAPDA
                     ALEAQAIAARTYLVRNLKRHAPGGFYLCDGQHCQAYGGMRYESSRSSLAVANTRGLVL
                     TYEGVVVNAVYHANCGGRLRSCDSVWGGVKVPYLPAHPDQFGHHSPFCQLPPQSKKSK
                     APLSHRPEARQYEPQTDRETDAAGWNSLLLWSEAKQATNRARGHGVGMCQDGAAGMAV
                     LGYNRNQILGFYYPGTRLARLGSSLLPSGETLPVEHDAPAGDRYAFLLKQPPITPPRI
                     PSRGVAGKTTATSQVVSSAQKLAKPPERANSSASGLNRWFWSNRPPTPNTVRAADGSL
                     KIAASKKKGKKNNGSEEKSVQKSVKTTKKSDASDKGKTKPKKKTDKKKVADEKVAKKS
                     DTVASSKKGTKSGDKDGSKSVRKKNDKKAAKSTGSVKKKGSDDGSKESQRTKEDKT"
BASE COUNT          565 a          984 c          833 g          727 t
ORIGIN      
        1 gattgggcca gtggatcaac cgtaaccggt aatatttttt cggcctgcgc aagacatctg
       61 caagaaaatc tttttacttt tcttcgccct gtgcccgttc ctgaaggatg gaccgcgcaa
      121 acgctctccc ctcgcggccg acagccccga gcatgcgggc gatttccgtc tctcgttttt
      181 tgccggtcac ttgtacaatc tcgatctcgg tctcgtcgcc ccgaacctgc ttgccgacga
      241 caaaatgcgt ctcgccctcc ttggcaatct gatgcagatg cgtcaccagc agaacctgct
      301 tctctttccc cagggccttc aggctcttcg ccaccttctc ggccgtcgtg ccaccaatgc
      361 cggtgtcgac ctcatcgaag atcagggtgg gaagttcgtc gtcgcgggcc agcacctttt
      421 tcagcgccag tgccacgcgc gacagttcgc cgccagacgc aatcttcttc aacggaccgg
      481 ccggagcgcc gggattgaac gacacctgaa actcgactgt ttcagcacct tcaggcccgg
      541 gttcgcattc ctgcaaggcc acctgcacgc gggcgcgcgg aaagccgagc ccttcgagct
      601 ctgctgtgac ctgcttcgac agcttctcgg cgagtttggt ccggcgttcg ctgagtttgc
      661 gacaacacgc ggaacagagt ttttcgacct cgcccagctc ttggcgtatt ttctcgagtg
      721 ccgtgtcggg ggctgtcaac tcaccgatct cctgcagggc ttcctgccga agggcgaaca
      781 acccctggca gtcgcggccg taccggcgct gcagacggcc gatgtcagcc agacgcgact
      841 gcagaaatga aagcctctcc ggatcaaaga ccaaccgtga cgcgaaacga tcgagctcgc
      901 gctccatgtc ggcggcttcg atctcgagcg actcgacacg ctgcagaaat tcctttgccc
      961 ccacatcgtg ttctgaagca cgttttaacc catcacgagc ggtacgtaac aacttcacga
     1021 tgccatcgag ttcttcagca cccgagagcg catctcgcgc ggtattcacg cccgaggcaa
     1081 tcgattcaac gtgcatgagt cggttcgctt cttccttcaa ctcctgctct tcttcgggcg
     1141 aattcagccc gagggcttcg agatctttca gcatgcgctc aagctcttcg atgcgggcgg
     1201 tgccgcgacc gagacgttct tccgtgactc gcaggttctc cgccagctcg cgtcgcctta
     1261 cgtacaagct cgtatactct gatagaaccc tggcgtgagc gtctccgccc gtgcgatcga
     1321 gtagacgtcg ctgaacgcga ttctcgagca gggtttgatg ttcattctga ccgtgaattt
     1381 ccatcaggtg aacgccgagc tgtcgcagag actgagctga cgcgagaatg ccgttcacca
     1441 gcaccttgtc gctcccctcg gttttaaagg tacgagcgat caccagacga tcggggttgt
     1501 cggagtcaga caagccgagt tcgtcgatgg ctgttttcag ctcgcgatga tctttcacgt
     1561 cgaacaccgc ctggactttt gcctgccccc gaccgggaag cacgagaccc gaccttcctt
     1621 tcttgccgag caacagtttg atcgattcga gcagcacgct cttgcccgca ccggtttccc
     1681 cggtaatcac gttcagacca gccccgaact ccagcaatgc ctttctgatg aagagaacat
     1741 tttcgagatg cagttcacag atcatgtctt gtcttccttc gttcgctggc tctctttcga
     1801 gccatcgtcg ctgcccttct tcttcacgct tcccgtcgat ttcgcagcct tcttgtcgtt
     1861 cttcttccgg acagactttg aaccatcttt gtcgccgctc ttcgtgccct ttttcgacga
     1921 agccacggtg tcacttttct tcgccacttt ctcgtcagcg actttctttt tgtctgtctt
     1981 ctttttcggc ttcgtctttc ccttgtccga agcgtccgac ttcttggtcg ttttcacgct
     2041 cttctgaact gacttttcct cagatccatt gttctttttt ccctttttct ttgaagccgc
     2101 gattttcaat gacccatctg ccgctctcac ggtattgggg gtgggcggcc ggttgctcca
     2161 gaaccagcgg ttcagaccgg acgcagaaga attcgcgcgc tctggtggtt tcgcgagctt
     2221 ctgagcactc gagaccactt gagacgtcgc cgtcgttttc ccggccacac cgcgagaggg
     2281 aatccgcggc ggagtaatgg gcggctgttt caagagaaag gcataccgat cgcccgccgg
     2341 agcgtcatgt tccaccggca gggtttcgcc ggaagggagc agcgaggaac cgagtcgggc
     2401 caaccgggtg cccggatagt agaacccgag aatctgattt cggttgtatc ccagcacagc
     2461 cattccggca gcgccgtcct gacacatgcc gacgccgtga ccgcgcgcac gattggtggc
     2521 ctgcttcgct tctgaccaca aaagcaggct gttccaaccg gccgcatcgg tttcacggtc
     2581 ggtctgcggt tcatattgac gcgcttcagg ccgatgggac aggggcgctt tggatttctt
     2641 cgactggggc ggcaactggc agaaaggtga gtgatgcccg aactggtccg ggtgtgccgg
     2701 aagatacggc actttgacgc cgccccacac ggagtcacag gagcgcagcc ggcctccgca
     2761 gttcgcgtga tagacggcgt tgacgaccac accctcatac gttagtacaa gtccacgagt
     2821 gttcgctacc gccagtgatg aacgtgaaga ctcgtaacgc ataccgccat aggcctggca
     2881 gtgctgcccg tcgcagaggt agaacccccc gggtgcatgc cgtttcaggt ttcgcaccag
     2941 atacgtgcgg gcagcgatcg cctgtgcttc gagcgccgca tcgggagcat ctttgccaac
     3001 ctcacagggc acgaccgacc gcaggtattc ttcggtgccg atgagattga ccaccaacat
     3061 gcgtccgccg aacggcacca gttcgatggc tccgcgatag acccgcaga
//
LOCUS       DAORHD010000509         3106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35880_length_3106_cov_3.351032, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000509 DAORHD010000000
VERSION     DAORHD010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35880_length_3106_cov_3.351032"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKO06_11230"
     CDS             <1..210
                     /locus_tag="PKO06_11230"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV70261.1"
                     /translation="IEAEDFPEKVRREQGSFDLGGFSEEKILSLEEMERMYVRKVVEY
                     TRGNKLQAARLLNIDPKTLRAKLGN"
     gene            220..516
                     /locus_tag="PKO06_11235"
     CDS             220..516
                     /locus_tag="PKO06_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNV70262.1"
                     /translation="MPDVQLLNRMEKVTADVADEMLQKSADFCRCSRCHLDVVALALN
                     SLPPRYVVTEIGEVFTNVNLASAQWRADVMTAVLQSMEIVRKKPRHNPKNGGTK"
     gene            513..896
                     /locus_tag="PKO06_11240"
     CDS             513..896
                     /locus_tag="PKO06_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV70263.1"
                     /translation="MTERKPGTDKEKPKLSFDQRNVEKGYVVLSLIGDLDMWTLPLAK
                     ERINQMVQEGKVRLVLDLEKMNYIDSSGLGFFIGTLKKLRDSGGDLKLINLNAYIYGI
                     FKLIQLQHIIQTYDSLEEASKQFMS"
     gene            915..1250
                     /gene="rpmE"
                     /locus_tag="PKO06_11245"
     CDS             915..1250
                     /gene="rpmE"
                     /locus_tag="PKO06_11245"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNV70264.1"
                     /translation="MKPKIHPEFQKINVTCACGNTFETMSTAKELRVEVCSGCHPFFT
                     GTQRLLDTTGRVEKFKQKIEAHKTHIAENPVKAKKPVTKPAAKPAPEAKKAKADTKAK
                     DAEKAAETK"
     gene            1334..2041
                     /gene="thyX"
                     /locus_tag="PKO06_11250"
     CDS             1334..2041
                     /gene="thyX"
                     /locus_tag="PKO06_11250"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNV70265.1"
                     /translation="MEVLLLRYTPDPDRIVALAARRCYSARAARAIDDRLSEEECDRL
                     LALLRRRNHLSPYEHASFTFSVDGVSRALSHQLVRHRIASYSQESQRYVDYHNLPQVP
                     CVTPPTIKARPEAKKVFEQVVQATLDAYRRLSELGVPPEDARYIFPNAIETKLVFTLN
                     ARSLFNFFEQRCCIKAQWEIRQLADQMLRLVREAAPRIFRNAGSPCRYEKPYCREDDA
                     ECKLFPQPREKEKGHDD"
     gene            2031..3101
                     /locus_tag="PKO06_11255"
     CDS             2031..3101
                     /locus_tag="PKO06_11255"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNV70266.1"
                     /translation="MTTDLKQIRQKIDQLDTEIVKLLESRAEAVIEAMNVKNESGAPA
                     YTPQREAEILKRLDKAKQKLGHAALRGIYAEILSACRALQGRQKVALLGEKFGWVHDA
                     ALKQLGSAVDVQAFEHPRDVIKSLNQGHANLGFFPLSGDLPDIRFLLEELLAQNYHIV
                     AETRVRRTFSLISRGATDIMKVSQILVTKEFFPLVRDWAQSISFPVKIVICRTVDEIV
                     ESLVDVSPIAGLIPAAVAKTIDANMLQEEIKASEEWPYRCLTLRREPEQHIGKGQRAA
                     VLFALQNNVGSMGELLDVLRAEKLQLYSIQTYFFGSKPWKDLFFMDFEAAVTADEQTR
                     ILKKLESKTLFLKNLGVYSVTE"
BASE COUNT          732 a          889 c          853 g          632 t
ORIGIN      
        1 atcgaggccg aagatttccc cgagaaagtc agacgggaac aagggtcctt tgatctcggt
       61 ggtttttctg aagagaaaat tttatcgctc gaagaaatgg agcgcatgta cgttcgaaaa
      121 gtagtagaat atacccgagg caataaactt caggcagccc gcctgctgaa tattgacccg
      181 aaaacgctgc gcgccaagct cggcaactga ggtgtaatcg tgccagatgt acaactgctg
      241 aatcgcatgg agaaagtcac cgcggacgtg gctgacgaaa tgcttcagaa gagcgctgac
      301 ttctgtcggt gttcacgctg ccacctcgac gtggtggcac tggctctcaa ttcgttgcca
      361 ccgcgatatg tcgtgaccga gatcggcgag gtgtttacca atgtcaatct ggcttcagcc
      421 cagtggcgtg ccgatgtcat gaccgccgtg ctgcagtcca tggaaatcgt ccggaagaaa
      481 ccccggcaca accccaagaa cggaggaact aaatgaccga acgaaaaccc ggaaccgaca
      541 aggaaaagcc caagctgagt ttcgaccagc ggaacgtcga aaaaggatac gtcgttcttt
      601 ccctgatcgg cgacctggat atgtggaccc tccctcttgc gaaggaacgc atcaaccaga
      661 tggtccagga ggggaaagtc cgactggttc tcgatctcga aaagatgaac tacatcgatt
      721 cgtcgggtct cggcttcttc atcggtacgt tgaagaagct ccgtgattcc ggtggcgatc
      781 tcaagctgat caatctgaat gcctacatct acggcatctt caagctgatt cagctgcagc
      841 acatcattca aacgtatgac agccttgaag aggcttcgaa acaattcatg tcctgagcac
      901 tggagaagta ttcaatgaaa cccaagatcc atcccgaatt tcagaagatc aatgttacct
      961 gcgcctgcgg caacaccttc gagaccatgt cgactgccaa agaactgaga gtcgaagtgt
     1021 gctccggttg tcacccgttc ttcaccggaa cgcagcgcct gctcgatacc accggtcgcg
     1081 tcgagaagtt caagcagaag atcgaagctc acaagaccca cattgctgag aacccggtca
     1141 aagccaagaa gcccgtgacc aagcccgcag ccaaacccgc tcctgaagcc aagaaggcga
     1201 aagccgacac caaggccaag gatgccgaga aggccgccga gaccaagtaa tcgcggttcc
     1261 tttcgaacca tgcaccagaa cggggcggcg atcgtgccgc cccgtttttt actggaaaac
     1321 cggacgctca ttcatggaag ttctgctgct tcgctatacc cctgaccctg atcgcatcgt
     1381 agctttggcg gctcgtcgct gttactctgc gcgtgccgca cgggcaatcg acgatcgact
     1441 ttccgaagag gagtgcgatc gtctgctggc cctgctcagg cgtcgcaacc atctttcgcc
     1501 ctacgaacac gcctcgttca cgttcagcgt cgacggggtt tcgcgagcgc tttcccatca
     1561 gctggtgcgt caccgtatcg cttcgtattc gcaggaaagc cagcggtatg tcgactatca
     1621 caacctgccg caggtgccgt gcgttactcc gccaaccatc aaagcacgac cagaagcgaa
     1681 gaaggttttc gaacaggtag tacaggcaac gcttgatgcg tatcgccgcc tgtccgaact
     1741 tggggtgccg cccgaagacg cacggtacat ctttcccaac gccatcgaga ccaagctggt
     1801 gtttacgctg aatgcccgca gtcttttcaa tttttttgag cagcgctgct gtatcaaagc
     1861 ccagtgggag attcgacagc tggccgatca gatgctgcgc ctggtgcgcg aagccgcgcc
     1921 gcgtattttc cggaatgccg gttcgccctg ccggtatgaa aaaccctatt gccgcgaaga
     1981 cgatgccgag tgtaagctat tcccgcagcc ccgtgagaag gagaaaggcc atgacgactg
     2041 atttgaaaca gattcgccag aagatcgacc agctcgatac cgagatcgtg aagcttctcg
     2101 agtcacgcgc cgaagccgtg atcgaagcca tgaacgtcaa gaacgagtcg ggagcaccgg
     2161 catacacccc gcagcgcgag gccgagatcc tcaagcgact cgacaaggcc aagcagaagc
     2221 ttgggcacgc ggctttgcgt ggcatttatg ccgagattct ctcggcgtgt cgcgcgctgc
     2281 agggccgaca gaaagtcgct ctgctcggcg aaaagttcgg ctgggtgcat gatgccgccc
     2341 tgaaacagct gggcagtgcc gtcgacgtgc aggccttcga acatccgcgc gatgttatca
     2401 agtcgctgaa tcagggccac gcgaatcttg ggttcttccc gttgtccggt gatctgcccg
     2461 acattcgctt tctgctcgag gaactgttgg cacagaacta tcacatcgtc gccgaaaccc
     2521 gcgtgcgccg caccttttcg ctgatcagtc gcggtgccac ggacatcatg aaggtgtcgc
     2581 agattctggt aaccaaggag ttcttcccgc tggtgcgtga ctgggcgcag agtatctcct
     2641 tcccggtcaa gatcgtcatc tgccgcaccg tcgatgaaat tgtcgaaagt ctggtcgatg
     2701 tcagcccgat tgccggcctg attccggcgg ccgtggcgaa gacgatcgat gccaacatgc
     2761 tgcaggagga aatcaaagcc tctgaggaat ggccctatcg ctgtctgacg ctgcgtcgcg
     2821 agcccgaaca gcatatcggc aagggacaac gcgccgccgt gttgttcgcc ctgcagaaca
     2881 acgtgggttc catgggagag ctgcttgacg tgctgcgggc cgaaaagctg cagctgtact
     2941 ccattcaaac gtacttcttc ggcagcaagc cctggaaaga cctgtttttc atggatttcg
     3001 aggcggcagt gaccgctgac gagcagacgc gcattctgaa gaagctggaa tcgaagaccc
     3061 tcttcttgaa aaacctcgga gtctactcgg ttaccgaatg accgcc
//
LOCUS       DAORHD010000510         3103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35934_length_3103_cov_3.825787, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000510 DAORHD010000000
VERSION     DAORHD010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35934_length_3103_cov_3.825787"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="PKO06_11260"
     CDS             complement(<1..1765)
                     /locus_tag="PKO06_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNV70267.1"
                     /translation="MQRWVRVRRVLVLWLLVVATWVGAAEMPRRVGVFPGSFDPFHVG
                     HLKVALEAASTLALDEVIIAPNATNIRKPGMSSLEFRWQAIHRALAELSDPRLSLLPI
                     EAIENADRAGPPETAPQRLFEAAARSRSGAAIYQILGSDSFVKIVENRGLPKPGDHRF
                     LIISMRPGEPLAYPSEVKRLQKLGLMHCIPEPNLDISSTRIRRAAREADYDFLRGHLP
                     VGTWRLVMKHGAYGLDAAPGWPQALLELGPDRGTVEDHPEELAEGWVKDYPLPDQATA
                     SDGFPLEFPDARVQAIDFTASHTLGDLRGYLEQRLTPLGMKLIERPDVEVYLVRGPRE
                     RVGRFLRRLEVTRALEVTRSRSYITLGVVFGKRADGRVVVAIGDVWGRDRDLHTQALI
                     GGALAVSGRPALGFRVVAYPAGADDARYMRDEYRRALQAIVPGTIDTAIVGYHWKVVQ
                     QLNWRLSTYRKFPNRWTMRDGRISTTWRYRIEKNTPETLPEEGWSRRYFDHRSLPFSL
                     LFYRDSAGRPRTILLTRNVYGDQLTALLGVLVEDKKIRNIRLLGSCGGLNEELRVGDL
                     VQPTETACDESTWSPAHVQPWS"
     gene            1967..2857
                     /locus_tag="PKO06_11265"
     CDS             1967..2857
                     /locus_tag="PKO06_11265"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV70268.1"
                     /translation="MAAPQNLSLEQALEALRGKDIARLSELLTGGAPLEQLVEGFTLL
                     HHAVHVHDLTAIALLLKHRAPLEATNRRKQTPLHRAAIFGNPAVCALLLSRGANPQAR
                     DSQGHTPLEYVMQSSGRVDLVRLFQAHGASLTARGKEGRTLLHWAAEQRHFQTARYLL
                     QQGLDVTAIDRLGRTALHLAVEFQARDIVRLLLESGATVDARTHAGLTPLHFAAALNR
                     EEIARVLLKHGASPMTTDLLGWTPLHEAAAWGSTNLVQLLIQAGADVSRQTAAGETPS
                     VLAHRFHHQDCVKILAVGTK"
     gene            complement(2818..>3103)
                     /locus_tag="PKO06_11270"
     CDS             complement(2818..>3103)
                     /locus_tag="PKO06_11270"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70269.1"
                     /translation="GPVLSGILGTPEVRLEYTVVGDTVNLASRLTDVALQQQGGGVVI
                     DSVFRDRCQSAPLDSKCHWEPRALNIHRVKGKARDVEIYFVPTARILTQS"
BASE COUNT          627 a         1002 c          843 g          631 t
ORIGIN      
        1 gactccaggg ctgcacgtgt gccggactcc aggtgctttc atcgcacgcg gtttcggtcg
       61 gctgtaccag gtcaccaact cgcagttcct cgttcagtcc gccacagcta cccagcagtc
      121 tgatgttcct gatcttcttg tcttcgacca gcaccccaag cagtgccgtc aactgatcgc
      181 catagacgtt gcgcgtgagc agaatcgttc gcgggcggcc ggccgaatca cggtaaaaca
      241 gcagactgaa cggcagtgac cgatgatcga agtagcgacg tgaccatccc tcttccggta
      301 acgtctcagg ggtatttttc tcgatccgat accgccacgt ggtcgagatg cgaccgtctc
      361 tcatggtcca tcgattcggg aacttgcgat atgtcgacag gcgccagttc agctgttgaa
      421 cgactttcca atggtacccg acgatcgccg tgtcgatcgt accgggaacg attgcctgca
      481 gtgcccgccg atattcgtcg cgcatgtaac gagcatcgtc ggcgccggcc gggtaagcca
      541 ctacgcggaa tcccaacgcc gggcgaccac tgactgcgag tgctcctccg atcagggcct
      601 gggtgtgcag atcgcggtca cgtccccaga catcacctat cgcgacaacc actctcccat
      661 ccgcacgttt tccgaacacg actcccagcg tgatgtacga ccgagaccgc gtcacctcaa
      721 gagcccgcgt cacctcgagt cgccggagaa aacgaccgac ccgctcccgt ggaccgcgca
      781 ccagatagac ctcgacatcc ggccgttcga tcaatttcat accaagaggc gtcagacgct
      841 gttccagata tccacgcagg tctccaaggg tgtgcgaggc ggtgaagtcg attgcctgaa
      901 cgcgggcatc cgggaactcc agcggaaacc catcacttgc ggtggcctga tccggaagcg
      961 ggtagtcttt cacccagcct tcggcaagtt cttccgggtg gtcttcgacg gttcctctgt
     1021 ccggtcccag ttcgagcagg gcctgcggcc aacccggagc agcatcgaga ccataggccc
     1081 cgtgcttcat gaccaggcgc caggttccta ccggcagatg acctcgcaga aagtcatagt
     1141 cggcttcgcg agccgcccgg cgaatgcggg tcgatgaaat gtccaggttt ggctcgggaa
     1201 tacagtgcat cagtccaagc ttctgcagac gtttcacctc actcggatac gcgagcggtt
     1261 cgcccggacg catgctgata atcaaaaatc gatgatctcc gggtttgggc aagccgcgat
     1321 tctcgacgat cttgacgaag gaatccgaac ccagaatctg ataaatcgcc gctccgctcc
     1381 gcgaccgtgc cgcggcctca aacaatcgtt gcggcgcggt ttccggtgga ccagcgcgat
     1441 cagcgttttc aatcgcctcg atcggcagca ggctcaagcg cggatcgctc agttccgcca
     1501 gagcccggtg aatcgcttgc cagcggaact caagagatga catcccgggt ttgcggatat
     1561 tcgtggcatt cggggcaatg atcacttcgt cgagcgcaag cgtcgatgcc gcttccaggg
     1621 ccactttcag gtggcccaca tggaacggat cgaagctgcc ggggaacact cccacccggc
     1681 gcggcatctc ggcggcaccc acccacgtcg ccacgaccag cagccagaga acaagaacgc
     1741 gacgcacccg aacccatcgt tgcataatca accctccacg aaatctgtct ggtattttcg
     1801 cctgattccc tcttttccgc aatccctgct ctctttgact tgcaaccccg tctcgatgat
     1861 aatctgagtt tccatgaacc acctgaaacg tcgcccgaac gcgagccccc tgttttcttt
     1921 cttttccttg ctcgcttgtg cggtgtttct tctcgggcaa cctgtgatgg ctgctcctca
     1981 gaacctttca ttagagcagg ctctagaagc actccgaggg aaagatattg ctcgtttgtc
     2041 cgagttactt acagggggag cgcctcttga acagctggtt gagggattca cgctcttaca
     2101 tcacgcagta cacgtgcatg acctcaccgc aatcgcgctt ctgctgaagc acagagcgcc
     2161 cctcgaagcc accaaccggc gaaagcagac cccactgcat cgagccgcca ttttcggcaa
     2221 ccctgcggta tgtgccctgc tcctgtctcg cggggccaac ccgcaggcgc gcgattccca
     2281 gggacacacc ccgctcgagt atgtgatgca gagcagcggt cgggtcgatc tggtcaggct
     2341 gtttcaggct cacggcgcga gtctcaccgc gcgcggcaaa gaagggcgca cgctgctgca
     2401 ttgggctgcg gaacaacgcc actttcaaac agcccgttac ctgctacagc agggccttga
     2461 cgtcacagcc attgatcgac tcggtcgtac cgctctgcat ctggcggtcg agtttcaagc
     2521 gcgcgatatc gttcgcctgc tgctcgaatc cggggcaaca gtagatgctc gcactcacgc
     2581 ggggttgacc ccgttgcact ttgccgcagc cctgaaccgg gaagaaatcg cccgggttct
     2641 tctgaagcat ggggcatctc ccatgacgac cgaccttctg ggctggaccc cgcttcacga
     2701 agctgccgcc tggggatcaa caaatctggt acagcttctg attcaggccg gagcagatgt
     2761 ttcacggcaa acggcggctg gggaaactcc gagtgtcctg gcccacaggt ttcatcatca
     2821 ggactgcgtc aagattctcg ccgtcggaac aaagtaaatc tcgacatcac gcgcttttcc
     2881 cttcacccgg tggatattca gagctcgcgg ttcccaatga cactttgagt ccaaaggtgc
     2941 cgactggcag cgatcacgga atacgctatc gatcacgact ccgccaccct gttgctgcag
     3001 cgcgacatcc gtcagccgcg acgccagatt caccgtgtcg ccaactactg tgtattccag
     3061 ccgcacttcg ggggttccga gaataccaga cagcaccggt ccg
//
LOCUS       DAORHD010000511         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35957_length_3102_cov_2.986544, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000511 DAORHD010000000
VERSION     DAORHD010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35957_length_3102_cov_2.986544"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="glyA"
                     /locus_tag="PKO06_11275"
     CDS             <1..1171
                     /gene="glyA"
                     /locus_tag="PKO06_11275"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNV70270.1"
                     /translation="LIASENFVSNAVLEAQGSVLTNKYAEGYPAKRYYGGCEVVDEAE
                     NLARDRAKKLFGAEHANVQPHSGSQANMAVYMSVLKPGDTYLGMNLQNGGHLTHGSPV
                     NFSGMLYKCVPYGVDEKTETIDYDEVEKLAKEHQPKLIMIGASAYPRIIDFERFRKIC
                     DQVKAVMVVDMAHIAGLVAAGLHPSPVPYADFVTTTTHKTLRGPRGGMILCKEAYAKA
                     VDKTIFPGIQGGPLMHVIAAKAVALEEALLPSFKEYQKRVLENAKALAAGLTKRGFRL
                     VSGGTDNHLMLVDMRPKKITGKVAEAALDKAGITVNKNTIPNDPESPFVTSGLRLGSP
                     AVTTRGFDAKAMDAVAELIDQVVKAPTDEANLKQVREKVMGICGRFPFYPEFLLK"
     gene            1205..1576
                     /gene="gcvH"
                     /locus_tag="PKO06_11280"
     CDS             1205..1576
                     /gene="gcvH"
                     /locus_tag="PKO06_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006273958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNV70271.1"
                     /translation="MKFTKDHEWIKVEGKKGIIGISEYAVKHLGDIVFVDMPKVGSSL
                     TKGKTMGVVESVKTVSDIYAPASGKVVKNNADLEADPAMLNQDPHGKAWIIEIELANP
                     GDVDDLMDKAAYDKYCSTLSH"
     gene            1748..3100
                     /gene="gcvPA"
                     /locus_tag="PKO06_11285"
     CDS             1748..3100
                     /gene="gcvPA"
                     /locus_tag="PKO06_11285"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNV70272.1"
                     /translation="MAGFVPSTPQEVTQMLQAIGVKRVEDLFGDIPEKYWLNKPLNLP
                     AGMSEAEVLDKLTDLAYQNIGTDEVTCFLGAGAYDHHIPVVIKHLLSRGDFFTAYTPY
                     QAEISQGTLQAIYEYQSLMCELTGMDVANASMYEGATALAEATHMAQVQTGRNKLVMF
                     DSVHPEYRMVTKGMLTYMGAKIVEVPSDDGVTDLNKLADAIDAETAAVVVQYPNFFGC
                     IEDLETIGNMAKAHGALFIVAAYPIALGLLRPPGECGADIVCGEGQSLGIPMGFGGPY
                     LGYIAVKDALIRRMPGRLAGVTTDNRGQRGFTLTLQAREQHIRREKATSNICSNQALM
                     ALNATIYLSLLGRDGLREVAVQSLTKAHYLKRELEKLPGLKLPFTAPFFNEFVVQVPR
                     SEKVLKKMRKEGILAGVPLDCWYPALQDHIVVAVTEKRTAPEMQEYIDVLGGLLKHDH
                     "
BASE COUNT          704 a          865 c          945 g          588 t
ORIGIN      
        1 actgattgcc tcagagaact tcgtttcgaa tgcggttctc gaggcccagg gatcggtact
       61 gaccaacaaa tacgcggaag gctaccccgc gaagcgctac tacggcggct gcgaggtcgt
      121 cgatgaggcc gagaatctgg cccgcgatcg cgccaagaaa ctgtttggcg ccgagcatgc
      181 caacgtacag ccccattcgg ggagccaggc caatatggcc gtgtatatgt cggtgctcaa
      241 gccgggtgac acttatctcg gcatgaacct gcagaatggc gggcatctca cgcacggcag
      301 cccggtcaac ttttcgggca tgctctacaa gtgcgtgcca tacggtgtcg acgagaagac
      361 tgagacgatc gattacgacg aggtcgagaa gctggcgaag gaacatcagc cgaagctgat
      421 catgatcggc gcgtcggcct acccccgcat catcgatttc gagcggttcc gcaagatctg
      481 cgaccaggtc aaagccgtca tggtcgtcga catggcgcat atcgcgggtc tggtcgctgc
      541 cggcctgcat ccgtcgccgg ttccctacgc cgacttcgtc accacgacga cgcacaaaac
      601 tctgcgcggg ccacgcggcg gcatgattct gtgcaaggaa gcctatgcca aagccgtcga
      661 caagacgatc ttccccggca ttcagggcgg acctctgatg catgtcatcg cggcgaaagc
      721 cgtcgccctg gaagaagcgc tgctgccgtc gttcaaggaa tatcagaagc gcgtactcga
      781 gaatgccaag gcactggctg ccggtctgac caaacgcggg ttccgtctgg tatcgggtgg
      841 tacggacaac cacctgatgc tggtcgacat gcgaccgaaa aagatcacgg gcaaagtcgc
      901 tgaagcggcg ctcgacaaag ccggcatcac ggtcaacaag aatacgattc cgaacgaccc
      961 cgagtctccg ttcgtgactt ccggtctgcg actgggcagt ccggcggtga caacccgcgg
     1021 gttcgacgcg aaagcgatgg atgcagtggc cgagctgatc gatcaggtgg tcaaggcccc
     1081 gaccgatgaa gcgaacctga agcaggtgcg cgagaaggtc atgggcatct gtggtcggtt
     1141 tccgttttac cctgagtttc tgttgaagta acgtacgcga cgtacatcta atcaaggaga
     1201 cgttatgaag ttcacgaaag atcatgaatg gatcaaagtc gaaggcaaga agggcatcat
     1261 cggcatctct gagtatgccg tgaagcactt gggcgatatc gtgttcgtgg atatgccgaa
     1321 ggtgggcagc agtctgacca agggcaagac catgggcgtc gtcgaatcgg tgaagacggt
     1381 cagcgacatc tatgcaccgg cttcgggcaa agtggtcaag aacaacgccg atctcgaggc
     1441 cgacccggcc atgctgaacc aggacccgca cggaaaagcc tggatcatcg aaatcgaact
     1501 ggcgaacccg ggcgatgtcg atgacctgat ggacaaagcg gcctacgaca agtattgctc
     1561 gaccttgagc cattgatgaa gcgttgagtt gaccccgtcg actcgcccat cacggcgatc
     1621 gacggggtgc tcgtttcttc tgaacgtaca acgtgcggga gactcaggcg gcgaggtgtc
     1681 gccggtatgg gaattccgcg tgcatgtcaa gactgacgac tgcggtcgtc ctacctgagg
     1741 aggaactatg gcgggctttg tgccttctac cccgcaggaa gtgacccaga tgctgcaagc
     1801 aatcggggtc aaacgggtcg aggacctgtt cggggatatt cctgaaaagt actggttgaa
     1861 caagccgttg aatctgcctg caggcatgtc agaggcagaa gtgttagaca aattgaccga
     1921 tctggcctac cagaacatcg gcaccgacga ggtaacctgc tttctcggag ccggtgctta
     1981 cgaccaccac attcccgttg tcattaaaca tctgctgtcg cgcggcgact tttttacggc
     2041 gtacacgccg tatcaggcag agatcagcca gggaacgctg caggcgatct atgaatacca
     2101 gagcctgatg tgcgagctta ccggcatgga cgtggccaac gcctcgatgt atgagggggc
     2161 aaccgctctg gccgaggcaa cgcacatggc tcaagtgcag acggggcgca acaaactggt
     2221 catgttcgat tccgtccacc ccgagtatcg gatggtgacg aagggcatgc tgacctatat
     2281 gggagcgaaa atcgtcgaag ttccctcaga tgatggcgtc accgatctga acaagctcgc
     2341 cgacgcgatc gacgcggaaa cggcagcggt cgtcgtgcag tatccgaact ttttcggctg
     2401 catcgaggat ctcgaaacga tcggcaacat ggccaaggcg cacggcgcgt tattcatcgt
     2461 agcggcctac cccatcgctc tcgggctctt gcgacccccg ggcgagtgcg gcgctgacat
     2521 cgtgtgcggc gaagggcagt cgctcggcat tcccatgggg ttcggcggcc cgtatctggg
     2581 ctatattgcc gtgaaagatg ccctgattcg gcgcatgccg ggccgtctgg cgggtgttac
     2641 caccgacaac cgcggacagc gcggtttcac gctgaccctg caggcacgcg agcaacacat
     2701 tcgccgcgaa aaagccacct cgaacatctg ttcgaatcaa gctctaatgg ctctgaacgc
     2761 gacgatttat ctgtcgctgc tggggcgtga cgggctgcgc gaagtcgccg tgcaaagcct
     2821 gacgaaggcg cactatctca aacgggaact ggaaaagctg ccggggctca agctcccctt
     2881 tacagcgccg ttcttcaatg agttcgtcgt gcaggttccg cgcagcgaga aggtgctcaa
     2941 gaagatgcgc aaagaaggca ttctggcggg agtcccatta gactgctggt atccggcatt
     3001 gcaggaccac atcgtggtgg cggtcaccga aaagcgcacc gcgcctgaaa tgcaggaata
     3061 catcgatgtg ttgggagggt tattgaaaca tgaccactga ac
//
LOCUS       DAORHD010000512         3101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_35981_length_3101_cov_3.211097, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000512 DAORHD010000000
VERSION     DAORHD010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35981_length_3101_cov_3.211097"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="pstA"
                     /locus_tag="PKO06_11290"
     CDS             complement(<1..698)
                     /gene="pstA"
                     /locus_tag="PKO06_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNV70273.1"
                     /translation="MTPEILRQNLRWHRFGAGLSKAATIGIALILFAILVFIFVRGIG
                     HLSPSFFLQSPIDLGREGGVFPIIVSTLVLCALALLIAVPLGVTTAVFLTEYTREGWV
                     TLLIRFGAECLAGIPSIIFGLFGFMLFVVSLGMGWSLLSGGLTLACMILPTIIRTSEE
                     AIKTVPRGLREVGYSLGATKSQIITRIILPQAKNGIVTGIILALGRAIGETAAVIFTT
                     GISIEMPTSLFDSAR"
     gene            complement(695..1537)
                     /gene="pstC"
                     /locus_tag="PKO06_11295"
     CDS             complement(695..1537)
                     /gene="pstC"
                     /locus_tag="PKO06_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HNV70274.1"
                     /translation="MQRREKRIQQALLAIACSSLAILALIIVFVFREGLPIIWKTGLS
                     QFLLGTHWGPTKGQFGIFPMVIGTVQVTLGAMAIGVPFALLVAIYLSEFSTPLTRTIM
                     KASIELLAAIPSVVFGFIGVIVIVPFIRETFGGTGFSVLSASLILGVMILPTVISIAY
                     ESLRAVPQAYREGALALGATPWQSTVRVVVPAARSGIVTAVILGMGRAIGETMAVIMV
                     AGNAVVIPTSPLHPCRTLTSNIAIEMAYASGSHQQALFATGVVLFVMIFLLNLLASTL
                     VKRR"
     gene            complement(1560..2432)
                     /locus_tag="PKO06_11300"
     CDS             complement(1560..2432)
                     /locus_tag="PKO06_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV70275.1"
                     /translation="MRRRPTVTGRSATLRLPSRPWAMILLVVQFFISAFFLVGCGNQG
                     HGVVIAGSTSVQPVMEKIADSYRKTFPGRKVTVEGGGSSAGIMAALTGTAHLGMSSRD
                     LKQDHADEKTLTAIPLALDAIAVIVNAENPIQSLTLAQLRELFSGKIRNWQDLGGKHR
                     EVHLVVREEGSGTRTAFEELVMKEGKTELPIDTYALVQDSTGGVREVVRTDPGAIGFI
                     SLGAVTKQIKAVHVDGVEPTIAAVKAQKYKLVRPFLLVTKGPPATDTQMLIDYALGSA
                     GVKLLLQEGLVGAH"
     gene            complement(2557..>3101)
                     /locus_tag="PKO06_11305"
     CDS             complement(2557..>3101)
                     /locus_tag="PKO06_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV70276.1"
                     /translation="TCLKVALKRPDVVTGLGLIAPFVQPRDPNESPSAIPGMAKGYFL
                     RSLLGIAMPFLAQSKLRQHLARVWEPAEIPSLTLEDELHQYTVFEILLATMNDKNDLL
                     KLFKEVQGKLGEIKCPSLAVAGEKDAVCDASEQLRVLGDKLPGLKSVKLADGGHGLPF
                     THAEQLSQAVIAEFGTGTTH"
BASE COUNT          727 a          922 c          876 g          576 t
ORIGIN      
        1 cgggcggaat cgaagagtga cgtgggcatc tcaatcgaga ttccggtggt gaaaatgacg
       61 gcggcggtct cgccgatcgc ccgaccgagc gcgagaatga tgccggtcac gatgccgttc
      121 ttcgcttggg gcagaatgat gcgggtgatg atctgcgact tggtggcccc gagcgaatat
      181 cccacctcgc gcaggcccct tggcactgtt ttgatcgctt cttctgaagt gcgaatgatc
      241 gtgggcagaa tcatacaggc cagcgtcagc ccgccggaaa gcagtgacca gcccattccc
      301 aggctaacga caaacagcat gaacccgaac aaaccgaaga tgatcgaggg aatgccggcg
      361 agacattcgg ccccaaaccg gatcaacagc gtcacccagc cttcgcgggt gtactcggtc
      421 agaaacacgg cggtggtgac tccgagggga acggcgatca acagtgcaag agcacacaga
      481 accagagtcg agacgatgat gggaaaaacg cccccttcgc gaccaagatc gatcgggctc
      541 tgcagaaaaa acgacggtga cagatgtccg atgccacgca cgaagatgaa gacgagaatc
      601 gcgaacagaa tcagggcaat accgatggtg gccgccttgc tgagaccagc cccaaatcta
      661 tgccagcgca gattctgacg cagaatttcc ggggtcatcg tcgtttcacc agagtcgaag
      721 ccaacagatt gagcagaaag atcatgacaa acaaaacgac cccggtcgcg aacaacgcct
      781 gctgatgcga gccggaggca taggccatct cgatggcgat gttactggtc agggttcggc
      841 acgggtgaag cggcgacgtc ggtataacga ctgcattgcc agccaccatg atcacggcca
      901 tcgtctcgcc gatggcccgt cccatgccga gtatgacggc ggtgacaatg ccagaccggg
      961 cggcaggcac caccacacgc accgtgctct gccacggagt cgcaccgagg gccagcgctc
     1021 cttcacggta ggcctgcggc accgcgcgca gcgactcata cgcgatgctg ataacggttg
     1081 gcagaatcat cacgccgagg atcagcgagg ccgaaagcac cgagaaaccc gtaccaccga
     1141 acgtttcacg aatgaaggga acgatcacaa tcacgccgat gaacccgaag acaaccgagg
     1201 gaatcgcggc cagaagttcg atcgacgcct tcatgattgt ccgagtgagt ggcgtcgaaa
     1261 actcagacaa atagatggcg acaagcagag caaaaggaac cccgatcgcc atggcaccga
     1321 gggtcacctg taccgtgccg atgaccatcg gaaagatgcc gaactgcccc ttggtcggcc
     1381 cccagtgggt tcccaggaga aactgcgata acccggtttt ccagatgatc ggcaaccctt
     1441 cacgaaacac aaagacgatg atcagcgcca gaatggccag tgacgagcag gcaatcgcca
     1501 acaacgcctg ctgaatgcgt ttctcgcgac gctgcagatg gccccgggtc tccgtgtggt
     1561 cagtgagcac cgaccaggcc ctcctgtaac agcagtttca cgccagcaga acccagggca
     1621 tagtcgatca acatctgagt atcagtcgcg ggaggtcctt tcgttacaag cagaaacggg
     1681 cgcaccagct tgtacttctg cgctttcacc gccgcgatcg tcggctccac gccgtccacg
     1741 tggaccgctt tgatctgctt ggtaaccgct ccgagtgaaa tgaacccgat cgctcccggg
     1801 tcggttcgca ccacttctcg aacgccgccg gtcgaatcct gaaccagagc ataggtatct
     1861 attggcagct cggttttacc ttctttcatg accagttctt caaacgcggt acgcgttccc
     1921 gagccttctt ctctgaccac gagatgcacc tcgcggtgtt tgcccccgag atcctgccag
     1981 ttcctgatct tgccgctgaa cagctctcgc agttgcgcaa gggtcaatga ctgaattggg
     2041 ttttccgcgt tgacaatcac ggcaatggca tcaagtgcca gagggattgc tgtcagggtc
     2101 ttctcgtccg cgtggtcctg cttcaggtct cgcgatgaca tgcccagatg ggcggttccc
     2161 gtaagggcgg ccatgattcc ggcagagctt ccaccgcctt ccaccgtcac tttccgaccg
     2221 gggaacgtct tgcgataaga atcggcgatt ttctccatga ccggctgcac cgaggttgag
     2281 ccggcgatga cgacgccgtg cccctgattc ccgcaaccta cgagaaaaaa cgccgaaatg
     2341 aagaactgca ccacaagcag gatcatcgcc cacggtcggg aaggcaaacg aagagtggcc
     2401 gatcggcccg tcacggtcgg tcgtcgtctc actcgcgcct cctgaaaatc cggagtcttc
     2461 gtcggcgcgg atagtagcag attctccgtt cctgcgcaag tcgcccttca gaggcgagca
     2521 accgcgaggg cattcaacac acgagatacg aaatgtctag tgtgtggttc cggtgccgaa
     2581 ttcggcaatg acggcctggg acagctgttc ggcatgcgtg aacggcaacc cgtgcccacc
     2641 gtcggcaagt ttgacggact tcagcccggg gagcttgtct ccgagaactc gcagctgttc
     2701 gctggcatcg cacaccgcgt ctttttcacc agcaacggca agagacggac atttaatctc
     2761 acccaatttt ccctgtactt ccttgaagag cttcagcaga tcattcttgt cgttcatcgt
     2821 cgccagaaga atctcgaaca ccgtgtactg atgaagttcg tcttccaggg tgagcgaagg
     2881 aatctctgcc ggctcccaga ctcgcgccag gtgctgcctc aacttcgatt gagcgagaaa
     2941 cggcatggca atacccagca gtgagcgcag aaagtatcct ttggccattc cgggaatggc
     3001 agacggcgat tcgttcggat cgcgcggctg cacaaacggg gcgataagtc caagtccggt
     3061 tacgacatca ggccttttca aagctacttt gagacaggta a
//
LOCUS       DAORHD010000513         3100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36004_length_3100_cov_3.432184, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000513 DAORHD010000000
VERSION     DAORHD010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36004_length_3100_cov_3.432184"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(333..938)
                     /locus_tag="PKO06_11310"
     CDS             complement(333..938)
                     /locus_tag="PKO06_11310"
                     /inference="COORDINATES: protein motif:HMM:NF028518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNV70277.1"
                     /translation="MSEYRSQIQNLFLSTVRKRHQPVEVVLDTGTCLRGIIKGFDQFS
                     VTIGFHDKLEVIYKSAILYITAMPKKMRPRPMGAPRPSGMGRPPRPRTGDVMDDRPPR
                     PRSSDLMDDRPPPRSRPSDLMDDRPPRPRSPDVTEDRPPRPRSAPEPVEERPARPRTR
                     PSSSTTERPPARTPPPRQFIDIPEDNDHEDPPPPKKTTRKS"
     gene            complement(963..1646)
                     /locus_tag="PKO06_11315"
     CDS             complement(963..1646)
                     /locus_tag="PKO06_11315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV70278.1"
                     /translation="MSEIDKLQFLRQVPLFASLNDKALLELGAITVEQQIPEKTTVFK
                     QGDPGDALYIIKSGKVNILVRNSSGQETVLVTLGKPAVIGDMAVIDDQPRSASVATVT
                     QTEFYVITKNDFKSLLANVPEISFQILKVMSERLRKTNMHLKELEASANQMQEVIRVI
                     TKIARKSNLLSLNASIEAARVGDAGRAFSVVATEMKKLAEDSAQEAQKIDKLLQTLQM
                     KTRALAGMK"
     gene            1797..3092
                     /locus_tag="PKO06_11320"
     CDS             1797..3092
                     /locus_tag="PKO06_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNV70279.1"
                     /translation="MMKLDQALHWLSSHEICRGPFGLHRMEYLMDKLDNPHREYACVT
                     IGGTNGKGSVTSLLDSIMSLTSEYQIGSTISPHLVGIGERIRLQGRSLPSEWWCKGVE
                     ALQEPVQIMSREPDLGAPSFFELVTALAFWAWRENDRDLAILEVGLGGRLDATNVAYG
                     EIAVITNIGTDHREYLGPDREAIAREKLGIVKKKSVLVTAEPDPAIRQIFVDTCKSRK
                     AELVLVQESHGFELVQSHARGHTLKLPDVTDTFEFPLPGRHQLTNLATALTVVDRLRQ
                     HGFEISPEAVQTGIQQVRWPGRLQWINGPVPMLLDGAHNQEGLQSLVAYLQEFPLPRP
                     AHLILGALHQKPVADMARALAPFADTLAFVPVPTGRSLNRDDFESQVAPVDSRWRWHD
                     SIDTAVQAGVSAGAQSMLLSGSLYLLAEYFRTHPEIYHP"
BASE COUNT          600 a          890 c          924 g          686 t
ORIGIN      
        1 cctgaagttc cgagggcaga cgcacccaga catagacatt cagcgtcttg ttgaatcggc
       61 acgaatactt cacgtttccg atgaccttga ccatggccgc cctccataca ccgtgcggtc
      121 acgtgtccgg tgaccgcgag cgcgtcgaca aggctcagag gcctgatacg aagcgtctct
      181 cttcattcat cggcagtcag gggcgttggt ttgagcgttt tgttccatgg tcgtcgttca
      241 acgccaggac tgacacgcga ccggaaaaga acgcgagccc cgggtatcga gggagtccgg
      301 ggctcggctg gtagacagga ttcagcttca gatcacgatt ttcgcgttgt cttcttgggt
      361 ggtggcgggt cttcgtgatc gttgtcttcg ggaatatcaa tgaactgtcg cggtggtggc
      421 gtgcgtgccg gaggtcgttc ggtggtcgaa gacgacgggc gggtgcgcgg gcgtgccgga
      481 cgttcttcca cgggttccgg tgctgaccgc gggcgcggcg ggcggtcctc ggtcacgtct
      541 ggagaacgcg ggcgaggtgg ccggtcatcc atcagatcgc tgggtcgcga acgcggcggt
      601 gggcgatcat ccatcaggtc actggagcga ggccgaggtg ggcgatcatc catcacatcc
      661 ccggtgcgcg ggcgcggcgg acggcccatg ccgctcggtc gtggcgcgcc cattggacgc
      721 gggcgcattt tcttcggcat cgccgtgatg tagagaatcg ctgatttgta gatcacttcg
      781 agtttgtcat gaaagccgat ggtcacggaa aactggtcga accctttgat gatcccgcgc
      841 aggcaggtgc cggtgtcgag caccacctcg acaggctgat ggcgcttgcg cacggtggaa
      901 agaaacaggt tctgaatctg agagcggtat tccgacatgt gggtgctcct ttgcgaaggg
      961 ctctacttca tgcccgccag agcgcgggtc ttcatctgca gcgtctgcag cagtttgtcg
     1021 atcttctggg cttcctgggc cgaatcttcg gccagttttt tcatttcggt ggcaacgacc
     1081 gaaaaagcgc gcccggcatc gccgacacga gccgcttcaa tcgaggcgtt gagtgacagc
     1141 agattggact tgcgggcaat cttggtaatg acgcgaatga cttcctgcat ctgattcgcc
     1201 gacgcttcga gctctttcaa atgcatgttg gtcttgcgca ggcgctcgct catgaccttc
     1261 agaatctgaa acgagatctc gggaacattg gccagcaggc tcttgaagtc gttcttggtg
     1321 atgacgtaga actcggtctg cgtgacggtc gcgacggagg cggatcgtgg ctggtcatca
     1381 ataaccgcca tgtccccgat aacggccggt ttgcccagcg tgaccagaac ggtttcctgg
     1441 ccggaactgt tgcggaccag aatgttgacc ttgccgcttt tgatgatgta cagcgcatca
     1501 cccgggtcgc cctgtttgaa gacggtcgtt ttttcgggaa tctgctgttc gaccgtgatg
     1561 gctccgagtt cgagcagcgc tttatcattc aggctggcga acagcggaac ctggcgaaga
     1621 aattggagtt tatcgatttc actcatggct ggtacggcag cctgaatcct agcatcgcaa
     1681 gcctgttctg tcaaggtcag ccgccagcta atgcttgatg gctggctcat caggactcac
     1741 tttcacggca tcggagaatc tggtatagtg agccactttc acaaaggctc tgacacatga
     1801 tgaagctcga tcaagcgcta cactggctct cgtctcacga gatctgtcga ggacccttcg
     1861 gtctgcaccg catggaatac ctgatggaca agctcgacaa cccacaccgg gaatacgcct
     1921 gcgtcaccat cggcggcacc aacggcaaag gctcggtgac gtctttgctc gactcgatca
     1981 tgtctttgac ttccgagtat cagatcggtt cgaccatctc gccacatctc gtcggcattg
     2041 gcgagcgaat tcgtctgcaa ggtcgttctt taccatcgga atggtggtgc aaaggagttg
     2101 aagcccttca agaaccggta caaatcatga gtcgcgaacc cgacctgggt gcccccagct
     2161 tcttcgaact ggtcaccgct ctggcgttct gggcatggcg cgaaaacgac cgtgatctgg
     2221 ccattctgga agtcggcctg ggtggccgac tggatgccac gaacgtggcg tacggtgaaa
     2281 tcgcggtcat caccaatatc ggcaccgacc atcgcgaata ccttggcccg gaccgcgaag
     2341 cgatcgcccg cgagaaactg ggcatcgtca agaagaaaag cgttctggtc accgccgagc
     2401 ccgacccggc cattcgccag atctttgttg acacctgcaa gagccgcaag gctgagctcg
     2461 ttctggtaca agagtcgcac ggttttgaac tcgtacaaag ccatgcccgc ggtcacacac
     2521 tgaaacttcc tgacgtcacg gacacgttcg agtttcccct gccgggtcga catcaattga
     2581 ccaatctggc cacagccctg accgtggtcg atcgcctgcg ccagcatggg ttcgagattt
     2641 ctcctgaggc ggtgcagact ggtatacagc aggtacgctg gcccggtcgc ctgcagtgga
     2701 tcaacggccc ggttcccatg ctgctcgatg gcgcgcacaa tcaggaagga ttgcagtcac
     2761 tggtcgccta tctccaggag tttcctctgc cgcgaccggc acacctgatt ctcggagcgt
     2821 tgcaccagaa acccgtcgcc gacatggcac gcgcattggc tccgttcgcg gatacgctgg
     2881 cgttcgttcc cgtgccgacc ggacgatcgc tcaatcgcga cgactttgaa tcccaggttg
     2941 cccctgtcga ttcgcggtgg cgctggcacg attcgatcga cacggcagta caagctggag
     3001 tcagcgccgg agcacaatct atgctgttaa gcggttcgct gtatcttctt gctgagtatt
     3061 tccgcaccca tccggagatc tatcatccat gatcaccacg
//
LOCUS       DAORHD010000514         3097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36067_length_3097_cov_3.050296, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000514 DAORHD010000000
VERSION     DAORHD010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36067_length_3097_cov_3.050296"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKO06_11325"
     CDS             complement(<1..916)
                     /locus_tag="PKO06_11325"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV70280.1"
                     /translation="MIRPGTWLLAIAFICASVAAGLASAAEKAPKAWTYMIFMNADND
                     LDPFADEDFTELMRVGSSDWLNIVTITDREKGPAALHYVEKGNNVTVRELGEIDMGDW
                     KTLVNFVKEVSTSYPARHYAVSLWNHGSGWKHTQKVFKGISYDDQSGNYITTAQLGQA
                     TREIKAILGKKLDLLTMDACLMQMMEIAWEVHKNVDYVVASEELEPGNGYPYDSHMAG
                     LKPYTTPREFVCHMVSSFKTAYNGGVYGTEDTTQSAIDCSRLPALKDAIDGWAKAAMS
                     GSYAQAYKQALLDVQKFDTKTNIDLWHLI"
     gene            1144..1995
                     /locus_tag="PKO06_11330"
     CDS             1144..1995
                     /locus_tag="PKO06_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNV70281.1"
                     /translation="MRTFIIFLVIFWCSPIPAFCQTNPYADQPVTMPTAVPIGVSDAA
                     VMPELPVTPANPLASATPDAMTAHDKLQDNLALFKNLLKAGKYREASIVLGRIQDDAK
                     DVTDKKTKRVFPGTWRKMKEGFIDQSRIDELKGTLASLKNQMYAECVAKMLKEARNHE
                     GVSSKEGPGGGTVACAWMVSKVLRAAGMVPAGWEELGALALTQRMVKEQNWKKVTPGK
                     GKPMTKALMKPGDIVFWDPSDHVGVYLGDGMAFSNSSSDREGKIHPVSGYYDGWVPRF
                     TVRPPGA"
     gene            2102..3034
                     /locus_tag="PKO06_11335"
     CDS             2102..3034
                     /locus_tag="PKO06_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70282.1"
                     /translation="MNPFNKRLVISFVLFCLAASPLLAESQAETTLSFEPGSGEKQFG
                     VSVGQEGPNIGPMSVLTSGEGVVVLDAIHHRLIRLDGRGTFNGTIPLPAGQYRDITRS
                     GNGGIWLVEANSRSVMRVAGGGIRRVFDVPRQEGFPRQLDYLLITRKHLVIMDYSDNH
                     LYFFTPAGTVERRVEVSAALGFALDQNNRICFLGLTQGGDSELDLYNRLVRIAEDGSC
                     EEKVLSGPSFEGARLLGFSQTGKPVVLGIASQDPFKRELFLVNADGTHELIDTITQRL
                     LFATRYGHCSGNTLWVNISPMSGRKILFRRYELK"
BASE COUNT          652 a          848 c          873 g          724 t
ORIGIN      
        1 tgatcaggtg ccagagatcg atgttggtct tggtatcgaa cttctgcacg tcgagcagag
       61 cctgtttata cgcctgagca tacgacccgg acatggccgc cttggcccag ccgtcgatcg
      121 catctttcag agcgggcagg cgactgcagt cgatggcgga ctgcgtcgtg tcttctgtgc
      181 catagacgcc gccgttgtag gctgtcttga acgatgaaac catgtggcag acgaactcgc
      241 gcggggtcgt gtacggcttc aggccggcca tgtggctgtc atacggatac ccattgccgg
      301 gctcgagttc ttctgaagcc acgacgtaat cgacattctt gtggacttcc caggcaatct
      361 ccatcatctg catgaggcag gcgtccatgg tgagcaggtc gagcttcttg ccgagaattg
      421 ccttgatttc gcgagtggcc tggccgagct gggcggtcgt gatgtagttg ccgctctggt
      481 cgtcatagga gatccctttg aagactttct gagtatgctt ccagcctgaa ccgtggttcc
      541 agagactgac cgcgtagtgc cgagccgggt acgaggtgct gacttctttc acgaagttta
      601 cgagcgtctt ccagtcgccc atgtcgattt cgccgagctc gcgcacggtg acattgttgc
      661 ccttctcaac gtagtgcaga gcggccgggc ccttttcgcg gtcggtaatg gtgacgatgt
      721 tcagccagtc actgctgccg accctcatca gttctgtgaa gtcttcgtcg gcaaatgggt
      781 cgagatcgtt atcggcgttc atgaaaatca tgtacgtcca ggctttcgga gctttttcgg
      841 cagcgctcgc caaccctgcc gccacgctcg cgcagatgaa cgctatcgct aacagccatg
      901 ttcccggtcg aatcatgctc gttactcctt ttgctatgcg caaaaccgct cgaataccga
      961 tgatattttc tcattatact gatcgcaccc gcccccccgg aagagtccga acggaattat
     1021 tggagagatg tgccatccga ggcgaagctt cagtacgaaa tcttcggtgg aaacttccgg
     1081 acgctcggct ggtagagtat aattagattt tacactgcca ggtattcacg aaggagggca
     1141 ccgatgcgca ctttcatcat ttttctggtc attttctggt gttcaccgat tcctgctttc
     1201 tgccagacga acccgtatgc agaccaaccg gtcaccatgc caaccgccgt tccgatcggg
     1261 gtcagcgatg cggcggtcat gccggaactg cccgttactc ctgccaaccc cctggcatcg
     1321 gcgaccccag atgcgatgac ggcccacgat aaactgcagg ataatcttgc cttgtttaag
     1381 aatctgttga aggccgggaa ataccgagaa gcttccattg ttctgggccg aatacaggac
     1441 gacgcaaaag atgtcaccga caagaaaacc aaacgggttt tccccggaac ctggaggaaa
     1501 atgaaggaag gtttcattga tcaaagccga atcgatgaac tgaagggcac actggccagt
     1561 ctgaaaaatc agatgtatgc cgaatgcgtg gccaagatgc tgaaggaagc cagaaatcac
     1621 gaaggggttt cgtccaagga aggccccggg ggtgggaccg tcgcgtgtgc ctggatggtc
     1681 agcaaagttc tccgtgccgc cggcatggta ccagccggct gggaagaact tggcgcgctt
     1741 gcccttaccc agcgaatggt gaaggagcaa aactggaaga aggttacccc cggaaagggc
     1801 aagcccatga ccaaagcgct tatgaagccg ggcgatatcg tgttctggga tcccagcgat
     1861 cacgttggag tctacctcgg agatggcatg gccttttcga atagttcctc agaccgtgaa
     1921 ggaaagattc atccggtgtc cggttactat gacgggtggg tgccacgatt caccgttcga
     1981 ccacccggag cttgagttca gtcatacgcg gctgcgaatg ctcttcgcgg ccgcttttct
     2041 tttgccagga attcgaatct tcaagtatgc tgatgccaat ccattcatca gggaaagcgg
     2101 aatgaatcct ttcaacaaac gtctggtcat ctctttcgtg ctcttctgtc tcgccgcaag
     2161 cccattgctg gctgaatctc aagccgaaac cactctctcc ttcgagccgg ggtcggggga
     2221 aaaacagttc ggagtttccg tcggccagga aggtccgaac attggcccga tgagtgtttt
     2281 gacgtcaggc gagggagtcg tggttctgga cgccatacat catcggctga tccggctgga
     2341 cggccgtggg acgttcaacg gaaccatccc tctgcctgcc ggtcagtatc gcgatatcac
     2401 ccgctctgga aatggcggga tctggctggt tgaggcgaac tctcgttccg tcatgcgcgt
     2461 ggcagggggt ggcatccgcc gggtgtttga tgttccgcgc caggaagggt tcccccgaca
     2521 gctggactat cttttgatca cccgaaagca tctcgtgatc atggattatt ccgacaacca
     2581 cctgtacttt tttacccccg cgggaacggt ggaaagacgc gtcgaagttt cagctgcact
     2641 gggattcgct ttggatcaaa acaaccgcat ctgctttctc ggactgactc agggtggaga
     2701 cagcgaactt gatttgtata atcggctggt ccgaattgct gaggatggaa gctgtgaaga
     2761 gaaagttttg tccggccctt ctttcgaagg ggcgcgtctg ctcgggtttt ctcagacggg
     2821 aaagcccgtg gttctgggca ttgcctctca ggacccgttc aagagagaac tgtttctggt
     2881 gaatgccgac ggcactcacg aactcattga caccatcact cagcgtctgc tgtttgccac
     2941 ccgctatggg cactgctcag gcaacaccct gtgggtcaac atctctccaa tgtccggcag
     3001 gaagatcctc ttccgccgct acgaactgaa gtagtccgcc gcgaccgtgc cctctcagga
     3061 aacgtcgctt tcgagaagca tacgcgcctg atcgatg
//
LOCUS       DAORHD010000515         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36172_length_3092_cov_3.415212, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000515 DAORHD010000000
VERSION     DAORHD010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36172_length_3092_cov_3.415212"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PKO06_11340"
     CDS             complement(<1..962)
                     /locus_tag="PKO06_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70283.1"
                     /translation="MDAKKKIWFPSAAERIAKMYQSIGRDFGSSDMTNTLDLTDPHSE
                     MPDSRSRPLVYMELINELRDLPADRESREQVFLALVAPNNAEGLFLDLEAEVFGMLLK
                     GMNTVISRELAAAGVTTLHPYRLTDYESAYIGIGAPVRLVRPALAKAAEQMRYVWQEG
                     ELDAIALPTNPLYYPKVPGQTLLFSLTDWTDVDRGSALFWKCRQELRQQYGCLWPPRL
                     PSFEHKCSGEGKLSFHDLVRQRAELWDSHGRQPADLAKGDFLVAMYCWLSSPGMMYCG
                     FDSLLADYWKQSHDQSESEYFAMLRKRFPCTECHETYMLDNLKI"
     gene            complement(1127..1783)
                     /locus_tag="PKO06_11345"
     CDS             complement(1127..1783)
                     /locus_tag="PKO06_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV70284.1"
                     /translation="MATVSQACIRRFAFARIDMATVSQYSDDMDQRYAIGQLVELTGT
                     SRRVIRYYIAQGLLEPPLGAGRGHYYTAKHLHRLRQIASLKQLGLTLDEIRRDSFLNS
                     DHPPAPVPVAKTRGRGHKSLRSELGSMQAHSLTEPRMSADACCSVYGESASEYAQRAA
                     AGELLEQSVVGPFEERWVRIPLANGVELHLQKRKLGHTPEELREIVRKISDLFRRPRL
                     "
     gene            1784..2911
                     /locus_tag="PKO06_11350"
     CDS             1784..2911
                     /locus_tag="PKO06_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70285.1"
                     /translation="MDHSPDAASLLDQITEHIRQYLKHARVSGTAIDPAALIMSVENT
                     LGWEARWLVPDAVKNLLCDSDSGQLFPTGQRAALCAESLRKALHGSEVPSRETQRTID
                     ALFHRSKQFSTTAEFQRVITFMGRFREYAPFNNLLVLVQRPTCSFYATERDWWKRFRR
                     TITATATPMVILAPMHPVLLVYDVNSTEGPPLPKELGAVHAVRGQPDPKIWSRLVRNC
                     YRHGIMVTLQPFSEGKSGFTRRSRNSKFKIVVSLDKKAEPAKRLAILCHEIAHVLLGH
                     VGGDSDGWWPNRPELTHAAVEIEAEATAFIVCETIGIETSSEKFLALKSRDLPEVPAE
                     VSIELIAKVAGHIKEMCTRLLPPPKVRQKKPETSEPSRAPG"
BASE COUNT          697 a          905 c          854 g          636 t
ORIGIN      
        1 cagattttca agttatctag catgtacgtt tcatggcatt cggtgcaggg gaaacgtttg
       61 cgcaacatgg caaagtactc agattcgctc tgatcatgcg attgtttcca atagtctgcg
      121 agcaatgagt cgaaaccgca gtacatcatc cccgggctcg agagccagca gtacatggcg
      181 accaggaaat ctcccttcgc cagatcggcg ggctgacgac catgcgaatc ccagagttcc
      241 gcgcgttgcc gtaccaggtc gtgaaacgac agctttcctt cgccactgca cttatgctcg
      301 aatgacggca ggcgcggcgg ccagagacaa ccgtattgct gacgaagctc ctgacggcac
      361 ttccagaaca gtgcagaccc gcgatcgacg tcggtccaat ctgtcagact aaacagaagc
      421 gtctgccccg gcaccttcgg gtagtacaaa gggttagtcg gcaatgcgat cgcgtcgagt
      481 tctccttcct gccagacgta gcgcatctgt tcggcggctt tggccaatgc cggccgaacc
      541 agccggacgg gagccccgat gccgatatag gcagactcat agtcggtcag acgatacgga
      601 tgcaacgtgg tcacaccagc ggcggcgagt tctcttgaga tgacggtgtt catgcccttc
      661 agaagcatgc cgaacacctc cgcctccaga tcaaggaaca gaccttcggc attgtttggc
      721 gcgaccagag ccagaaacac ctgctcgcgg gattctctgt ccgcgggcag atcgcgcaat
      781 tcattgatca gttccatgta gaccaacgga cgactacggc tgtccggcat ctccgagtgg
      841 gggtccgtca gatcgagagt gtttgtcata tctgaagaac cgaagtcgcg gccgatggac
      901 tgatacatct tcgcgatgcg ttcggctgcc gaagggaacc agatcttctt ctttgcgtcc
      961 atacccacct ccatgctgat tttgatgttt tcaaaacgtt cagaagagaa cagtgaactt
     1021 tccgtctgaa agcaaaacgg atgcagggac agggccatcg ggtgcagaaa tccgctcgac
     1081 ggctgagaaa cgccgtcggt gagcgaacag atctacaggg gcgcgactac aaacgcggcc
     1141 gccgaaacag gtcggaaatc tttcttacga tctcacgcag ttcttctggg gtgtgtccta
     1201 gttttcgttt ctgcagatgc agttcaacac cattggccaa cgggattcta acccatcgct
     1261 cctcgaaagg tccaacaaca gattgttcca agagttcacc ggcggcggca cgctgcgcat
     1321 actctgacgc actctctcca tatacgctgc aacacgcatc ggccgacatg cgcggttcgg
     1381 tcagggaatg cgcctgcatc gaaccaagtt cggagcgcaa gctcttgtgg ccgcgaccgc
     1441 gagtcttcgc gaccggaacc ggagccgggg gatgatcact gttcaaaaac gaatcccgtc
     1501 gaatctcatc gagcgtcaga ccgagttgtt tgagagaagc gatctgacgc aggcgatgca
     1561 gatgtttggc cgtgtaataa tgtccacgtc cggcccccag aggtggttcg agcaagccct
     1621 gggcaatata ataccgaatc acccgacgac tggttccggt cagttcgacg agctgcccga
     1681 tagcgtagcg ttgatccata tcatcactgt attgtgacac tgtcgccatg tcaatgcgcg
     1741 caaacgcaaa ccggcgaatg cacgcctgtg atacagtagc catatggatc actcccctga
     1801 cgctgcttcg cttcttgacc aaatcaccga acacattcgt cagtatctga agcacgctcg
     1861 agtttccgga accgcgatcg acccggcagc gctgatcatg agcgtcgaga atacgttggg
     1921 ttgggaagcc cgctggctgg ttccggatgc cgtgaaaaac cttctctgcg acagcgattc
     1981 cggccagctt tttccgaccg ggcagcgggc cgctctttgt gccgagtctc tgcgaaaagc
     2041 cctgcatggg tctgaggttc cgagtcggga aacccagcgc acgatcgacg cactgtttca
     2101 tcgcagtaag cagttttcga ccaccgccga gtttcaacgg gtgattactt tcatggggcg
     2161 atttcgcgag tatgccccgt ttaacaatct gctggttctg gttcagaggc caacctgctc
     2221 gttctacgcc accgaacgcg actggtggaa gcgattcagg cgcaccatca cggcaacagc
     2281 gacaccgatg gtcattctgg cgccgatgca tccggttctg cttgtctacg acgtgaattc
     2341 gacggaaggc cctccgctgc cgaaagaact gggagccgtt cacgcggtgc ggggccaacc
     2401 tgacccgaag atctggagcc ggctggtacg gaactgttac cggcacggca tcatggtcac
     2461 cctgcagccg ttttcggaag gcaagagcgg attcacccgc cgcagccgaa actcgaagtt
     2521 caagattgtc gtcagcctcg acaagaaagc cgagccggca aagagactgg cgattctctg
     2581 ccatgaaatc gctcatgtgc tgctgggtca tgtgggaggc gactcggacg gatggtggcc
     2641 caaccggccg gagctcaccc atgccgccgt cgagatcgaa gcggaagcga cggcattcat
     2701 cgtctgtgaa acgatcggca ttgagacctc ctcggaaaag tttctcgctc tgaagtcccg
     2761 cgatctgccg gaagtgccgg ctgaggtcag catcgaactg atcgccaaag tcgccggcca
     2821 catcaaagaa atgtgtacca gattactgcc cccgccgaag gttcgtcaga agaaacctga
     2881 aacttcagaa ccttcccgag caccgggttg agtgcagtct ttcgaggtgt catcggtccg
     2941 acgcaaagcg gacgtttgca tagcacgaca cccgtcgacc ctgcgtttgg gtatgacccg
     3001 accctattcc ggctacccgg gcttccgcaa acactcgatc acattcaaca ttcagctctt
     3061 ccacgaccag cgcaactcga cgggcaaact cg
//
LOCUS       DAORHD010000516         3086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36292_length_3086_cov_3.937314, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000516 DAORHD010000000
VERSION     DAORHD010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36292_length_3086_cov_3.937314"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PKO06_11355"
     CDS             <1..239
                     /locus_tag="PKO06_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV70286.1"
                     /translation="RYGRDPDSFAAHAYDTVRLLESAWRQAHGSRDSLREALARTRLF
                     RGATGSMGFDETGNDTRLVRLAVCRNGSLVRIDE"
     gene            236..2728
                     /locus_tag="PKO06_11360"
     CDS             236..2728
                     /locus_tag="PKO06_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV70287.1"
                     /translation="MKRLGQPVTLIAACGVVFLFIGMLLSQSDWIPLQMPYGNFDVAG
                     LRSFVNRLAPGVFDHASLKLYTFDQWHWHPLIVMLGVIFPVFLIAGLWRNEEFWRWGS
                     SRPLVQWVTFVTARLGIIRVSGAWPLKRSCLGTFPFLNCQACEMASGACPIGVLQHHL
                     KWGVWPFSLLGSMLLFGVVLGKSICGWLCPFGFFSDLMDRLSLRLVKIPRVLGYLRYW
                     VLALVFIGTFLYLQFGIIDRNFFCSTLCGSGKIYGLIPYWASTASGSFLPVSGWIPDL
                     MGVGMTLGCQVLLTLLLLVAMLLVSGRVFCRVLCPLGGFWGLFYPVSLVGIRHDSRSC
                     SGCGACERTCPMGVSQRFEGFLDQSSCMSCGRCVKVCLSGSRRYRQELGLSLGNVEVS
                     MPEQKPDFHYGSLYNRIRKDIYVAAITLLSRTPMATARYAWEQTDFYRKLYGDQLPDQ
                     FDELPLTLKSEIGACDPYDVLAREMTDQVVYYGETTGSTGKPTPSFYTMREFHAARIL
                     SRITPYIGALDQVLAENRAVVNGLTFGFTIAGMSFGDFLQANGGMVANIGTRSTIAPP
                     ERMVRALKRLRPSVITGTEIDLLCWLKILQEDYPQDAERVREHLKVAMSTAELCSVSR
                     SRAIEREFGLVHVDIYACVEGFFSVPCPCGEKHILPLYHTEVLDPELKHATEYGEGRF
                     AFTNLIKKSSPLVRYLLDDYVTISISQCPHGFKKSIVPHGRWELTVQVGGRRCGVRHF
                     EEAIFKHGLFGDYRVVLEDGRMNVTVETFGRHEPAKTIEEALGAEFGMTTVVELVPFG
                     TITAYREVRKSKPILKIEDRRSCSTQKIPEYL"
     gene            2741..>3086
                     /locus_tag="PKO06_11365"
     CDS             2741..>3086
                     /locus_tag="PKO06_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV70288.1"
                     /translation="MDQSPKICPKCQQANFPIAEFCRHCGEAIYDLGTMSRGYSLTRL
                     SGYLVDGLFWGFEELARWWELSQLNQALKKLQRRRQQFMERMDEETGGELVDDADRAQ
                     LMSLTEEISQVSV"
BASE COUNT          568 a          811 c          934 g          773 t
ORIGIN      
        1 ctcggtatgg ccgcgacccg gattcctttg ccgcgcacgc gtacgatacc gttcgcctgc
       61 tggagtcagc ctggagacag gcgcacgggt cgagagactc tctgcgtgaa gctctggccc
      121 gaacgcgtct gtttcgcggt gcgacgggga gcatgggctt tgacgagacg ggaaatgata
      181 cccgcctggt tcgattggca gtctgtcgaa atggttctct ggtgagaatc gacgaatgaa
      241 gcgactcgga caaccggtca ctctgattgc tgcctgcggc gtcgtcttcc tgttcatcgg
      301 tatgctgttg tcgcaatcag actggatccc actgcagatg ccgtatggca actttgatgt
      361 ggccgggttg cgttcgttcg tcaaccgcct ggctccaggg gtctttgacc atgcctcgct
      421 gaagctctac acctttgatc aatggcactg gcatccgctg atcgtgatgc tgggcgttat
      481 ctttcccgtt tttctgatcg cggggttgtg gcggaacgag gagttctggc gatggggttc
      541 ttcgcgtccc ctcgtgcagt gggtaacctt cgtcacggca cgtctgggca tcattcgtgt
      601 gtcaggagcg tggccgctca agagatcgtg tctgggcacg tttccgtttc tcaactgcca
      661 ggcttgtgaa atggcgagcg gagcgtgtcc catcggcgtt ctacagcatc atttgaagtg
      721 gggagtgtgg cctttctcgt tgttggggtc catgctgctc ttcggagtgg ttttgggaaa
      781 atccatttgt ggctggctct gtccattcgg ctttttcagc gatttgatgg atcggctcag
      841 tctgcgtctc gtgaaaatcc cccgcgtcct gggatacctt cgatactggg tgttggcgct
      901 tgtgttcatt ggtacattcc tgtatctgca gtttggcatc atcgatcgga actttttctg
      961 cagcaccctg tgcggttcag gaaaaatcta cggattgatc ccctactggg cgagcacggc
     1021 ttccgggtcg ttcctccccg tgtcgggctg gatccctgat ttgatgggcg tcggcatgac
     1081 tctgggctgc caggttctgt tgactttgct gctgctggtt gctatgttgc tggtttccgg
     1141 gcgcgtattc tgccgggttc tctgtccgct cggcggcttc tgggggctgt tttacccggt
     1201 cagcctggtg ggaattcgtc acgacagccg ttcctgcagc ggctgcggtg cgtgtgagcg
     1261 cacgtgtccg atgggggtca gtcagcgttt cgagggtttt ctggatcagt cttcctgcat
     1321 gtcgtgcggg cgctgtgtca aggtgtgtct gtcgggttcg cggcggtatc gtcaggaact
     1381 tggcttgagt ctggggaatg tggaggtctc aatgcccgaa cagaagccgg attttcacta
     1441 cggctctttg tacaatcgca ttcgcaagga tatctatgtc gcggcgatta ccctcttgtc
     1501 gcgaactccc atggccacgg cccgctacgc ctgggaacag acggatttct accgcaaact
     1561 ctatggcgac cagctgcctg accagttcga cgagttgccc ctgactttga aatcagagat
     1621 cggtgcctgc gatccctatg atgtgctcgc ccgcgaaatg accgatcagg tggtctacta
     1681 cggtgagacc acgggctcga ccgggaaacc gaccccctcg ttctatacca tgcgcgagtt
     1741 ccatgcggcc cgtatcctct cgcgaattac tccctatatc ggggcccttg accaggtact
     1801 ggcggaaaat cgggcggtgg taaacggcct gacgtttggc ttcacgattg ccggcatgag
     1861 cttcggtgat tttctgcagg cgaatggcgg aatggtcgcg aatattggca cccgctcgac
     1921 gattgcacct cccgagcgaa tggttcgagc gctgaagcgc cttcgcccgt cggtcatcac
     1981 gggcaccgaa atcgatcttc tgtgctggct gaagattctg caggaagact atcctcagga
     2041 tgccgagcgc gtgcgcgagc atctcaaggt cgccatgtct acggctgagc tgtgcagtgt
     2101 ttcacgcagc agagcgatcg agagggaatt cggtctggtt catgtcgata tctatgcctg
     2161 tgtcgagggt ttcttttcgg tgccgtgccc atgcggtgaa aagcacattc tgccgctgta
     2221 tcacaccgag gttctcgatc ccgaactcaa acatgcgacc gagtatggtg aagggcggtt
     2281 tgcctttacc aatctgatca agaaaagctc accgctggta cgctatctgc tcgatgatta
     2341 cgtgaccatc agcatcagcc agtgtcccca tggtttcaag aaaagcattg tccctcatgg
     2401 gcgatgggaa ctgacagtac aagtcggtgg gcgtcgatgt ggcgttcgac actttgaaga
     2461 agcgatcttt aaacacggat tgttcggaga ctatcgcgtg gtgctcgaag atggtcgcat
     2521 gaacgttacc gtagagacgt tcggccgcca tgagccggcg aagacgattg aagaagcatt
     2581 gggggccgaa ttcggcatga cgaccgtggt cgaactggtc ccgtttggta cgattaccgc
     2641 gtatcgtgaa gtgcgcaaga gcaaacccat tctcaagatc gaagatcgcc gcagttgctc
     2701 gacgcagaag attccggaat acctgtaggc tggagggacc atggatcaaa gccccaaaat
     2761 ctgcccgaaa tgtcagcagg cgaactttcc cattgccgaa ttctgccgcc attgcggcga
     2821 agcgatctac gatcttggta cgatgtcgcg cgggtattcg ctgacgcgtc tgagcgggta
     2881 tctggtggat ggtctgttct ggggtttcga agaactcgcc cgctggtggg aactgtcgca
     2941 actgaatcag gccttgaaga aactgcaacg tcgtcgacag cagttcatgg aacgcatgga
     3001 tgaagaaacc gggggtgaac ttgtcgatga cgctgatcgc gcacagctca tgtcgcttac
     3061 cgaagagata tcgcaagtca gcgttc
//
LOCUS       DAORHD010000517         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36315_length_3085_cov_3.181848, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000517 DAORHD010000000
VERSION     DAORHD010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36315_length_3085_cov_3.181848"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKO06_11370"
     CDS             <1..733
                     /locus_tag="PKO06_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70289.1"
                     /translation="FLKGHYHEAHAHFLRFLQEWPAHALGAKALYFQALSACRFEPLE
                     TVQRLRDQAASLTVTLDALKDKIPEPDLVEMRVTLAGWKMQLGEHTASDPSFLALPPQ
                     TLDHWLRRGWFPPKPMNPVELLDWSNRWRTGAKTISDPLLRARLALLDAKQLWKIWCA
                     PLPKRALTNKLKAWGYFPLEQGLSQMLQQALHHGDPETKRQAALIGLSFDQQRHGPRF
                     SLESSPWFAYLKQRGIHEAEAWLPE"
     gene            730..1323
                     /locus_tag="PKO06_11375"
     CDS             730..1323
                     /locus_tag="PKO06_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70290.1"
                     /translation="MRSFFLLLTLLGAGVGALMYLKGCDAPVDFAGIRFSFHPTMTGG
                     EGKKQVVKKPLTASEARALEAKEMAERGLVQSTARAQEYLTTATNDYFQGQLQDALLR
                     LERAKWHNPYNYGVFRLSGQIFLELNQYRKAFNDWSRAAQLPNDDQYLKDDYNVLKRV
                     LTYNRQEMDRLQRRVNRNPDDLVAKEHLKELELQMQE"
     gene            1578..2531
                     /gene="floA"
                     /locus_tag="PKO06_11380"
     CDS             1578..2531
                     /gene="floA"
                     /locus_tag="PKO06_11380"
                     /inference="COORDINATES: protein motif:HMM:NF010186.0"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HNV70291.1"
                     /translation="MGTVIAAGAIVVFFFVLFVIIWMFPFGIYIRCMSAGVPANPMTL
                     VAMKLRNVPPDLIVDSYIKGNKGGLELYLDRLEAHYLSGGNIALVVDALISAARAHIN
                     LTFERAAAIDLAGRDVYDAVRMSVEPRVISTGEISGIARNGVEVIAKAKITVRANLAT
                     MVGGAGEETILARVGEGIVSAIGSAETHLAILKKPELITQCIMEAGLDSNTAFEIVSI
                     DIADIDVGRNIGAMLQNQQAEADKKVAQAKAEGRRALAIAQTQENKALEQEMKAKLVE
                     AEMVVPTTLGTCFKQGRLLSDRKKGKKKDKTSGFGLGADHG"
     gene            2524..2763
                     /locus_tag="PKO06_11385"
     CDS             2524..2763
                     /locus_tag="PKO06_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70292.1"
                     /translation="MDEPVLTRDQLEKINRQHRLELRQIRRMNEQQFQAFKKNFSVGL
                     LDNITRVEAEELLVSMLALNISMQNDINGSKKSVS"
     gene            2760..>3085
                     /locus_tag="PKO06_11390"
     CDS             2760..>3085
                     /locus_tag="PKO06_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HNV70293.1"
                     /translation="MRAKSERGFLLLLALLLSLTVSAAAIDPLTLKAGVWNKTQYSCV
                     IRSLLEDRHGTLWIGTFGAGLWRFDGQTVASVPANADGLPDSRISKLLLDGDRLFLQT
                     AGILDHL"
BASE COUNT          647 a          845 c          893 g          700 t
ORIGIN      
        1 ctttctgaaa gggcattacc acgaagcgca tgctcatttt ctgcgatttc tgcaggaatg
       61 gcccgcgcat gcgcttggcg cgaaagccct gtattttcaa gcgctttcgg cctgtcgttt
      121 cgagcccctc gaaaccgtgc agcgcctgcg tgatcaggcc gccagtctga cggtgactct
      181 cgatgcactg aaagacaaga ttcccgagcc tgatctggtc gaaatgcgtg tgactctcgc
      241 cggctggaaa atgcagcttg gtgaacacac ggcttccgat ccctcgttcc tggctttgcc
      301 gccgcagacc cttgatcact ggttgcgtcg cggctggttt cctccgaaac ccatgaaccc
      361 ggtcgaactg ctcgattggt caaatcggtg gcgtacggga gcgaaaacca tttctgatcc
      421 gcttctgcgg gccaggctgg cgttgctcga tgccaaacag ctctggaaga tctggtgcgc
      481 ccccttgccg aaacgcgctt tgacgaataa gctaaaagcg tgggggtatt ttccgctgga
      541 gcaagggttg tctcagatgt tacagcaggc gttacaccat ggtgacccgg aaaccaagcg
      601 acaggccgct ctgatcggac tttccttcga tcagcagcgt cacggccccc gtttttccct
      661 cgagtcctct ccgtggttcg cgtatttgaa acagcgcgga atccatgaag cggaggcgtg
      721 gttgccggaa tgagaagctt ctttcttctc ctcacactgc tcggagctgg cgttggcgcc
      781 ttgatgtacc tcaaggggtg cgatgctccc gtcgattttg ccggtattcg gttcagtttt
      841 caccccacca tgactggcgg cgaaggaaag aaacaggtgg tcaagaagcc cttgaccgcc
      901 tctgaggccc gcgcccttga agcgaaagaa atggctgaac gtgggctggt acagagcacg
      961 gcacgcgctc aggagtatct gactacagcg accaatgact attttcaggg gcagttgcag
     1021 gatgcgctgc ttcgcctcga gcgggccaag tggcacaacc cctacaatta tggcgtgttc
     1081 cgactgtcgg gccagatttt tctcgagttg aaccagtatc gcaaggcctt caacgattgg
     1141 tctcgggctg ctcagctgcc gaatgatgat cagtatctga aagatgacta taacgtgctc
     1201 aagcgcgttc tgacctacaa tcgccaggaa atggatcgtc tgcagcgccg cgtgaatcgt
     1261 aatccggacg atcttgtcgc caaagagcac ctcaaggaac tcgaactgca gatgcaggaa
     1321 tagggcgatt tctgccctcc cgtgccttca ggctctcgag tccgagccga cgatccccga
     1381 tgtgcgacag gcttcgaata ttctctgatt tcgtcttggt catagttgtt ctccggcagt
     1441 tctcagggcg agttgactgg actgcaggtg aaaaatcgtg acgacgaaaa aacggtcggt
     1501 cgcgcacatt gacagggcgg gagaaccggg ataaagtaat cggtagtcct cgcataggaa
     1561 taggggaggc aacgttcatg ggcactgtca tcgccgcggg cgccattgtt gttttctttt
     1621 tcgtcttgtt cgtcatcatc tggatgttcc cgttcgggat ttacattcgg tgcatgtcgg
     1681 cgggtgttcc cgcgaacccg atgacgctgg tggctatgaa gctgcgcaat gtgcctcccg
     1741 atctcattgt cgattcttac atcaaaggca acaagggcgg tctcgagctg tatctcgacc
     1801 ggcttgaagc ccactatctg tcaggcggca acattgcgct tgtggtcgat gcgttgatca
     1861 gtgccgctcg agctcatatc aatctgacct ttgaacgggc cgcggccatc gatctggcgg
     1921 ggcgcgacgt ctacgatgcg gtgcgaatga gcgttgaacc tcgcgtgatc tcgaccggtg
     1981 agatttcggg cattgcccgc aatggggttg aagtcattgc caaagccaag atcaccgtgc
     2041 gcgccaatct ggcgaccatg gtgggtggtg caggcgaaga gaccattctc gcccgcgtcg
     2101 gcgagggcat cgtgtccgct attggttcgg cagagacgca cttggccatt ctcaagaaac
     2161 ctgagttgat cacgcagtgc atcatggaag caggattgga ctcgaatacc gcgttcgaaa
     2221 tcgtctcgat cgatatcgct gacatcgacg ttggccgcaa cattggcgcc atgttgcaga
     2281 atcagcaggc cgaggccgac aaaaaagtcg cgcaggcgaa agccgaaggc cgccgtgcgt
     2341 tggccattgc ccaaacccag gagaataagg ctctcgagca ggagatgaag gcaaagctgg
     2401 tcgaggccga gatggtcgtg ccgacgacgc tcggtacctg cttcaagcag ggtcgcctgc
     2461 tttccgatcg caagaagggc aagaagaagg acaagacgtc cggttttggc ctgggcgctg
     2521 accatggatg agccggtcct gacccgtgat cagctcgaaa aaatcaatcg ccagcatcga
     2581 ttggagctgc gacagattcg gcgtatgaat gagcagcagt tccaggcttt caagaagaat
     2641 ttttccgtcg gtctgctcga taacatcacc cgggttgaag ccgaagaact gctggtctcg
     2701 atgttagctc tcaatatctc gatgcagaac gatatcaacg ggagcaagaa gtcggtctca
     2761 tgagagcgaa gagcgagcgc ggtttcctgt tgctgctggc gctgctcctg tctttgacgg
     2821 tctctgcggc agccattgat ccgttgacgc tgaaagccgg cgtctggaat aaaacgcagt
     2881 attcctgtgt cattcgatcg ctgctcgaag atcgtcacgg cactctctgg atcggcacct
     2941 tcggtgcggg cctctggcga tttgatggac aaaccgtcgc ctctgttccc gcaaatgctg
     3001 atggtctacc ggattcgcgc atcagcaagc ttctgctcga cggcgaccgc ctctttctcc
     3061 aaaccgccgg aattctggac catct
//
LOCUS       DAORHD010000518         3085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36319_length_3085_cov_2.778878, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000518 DAORHD010000000
VERSION     DAORHD010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36319_length_3085_cov_2.778878"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PKO06_11395"
     CDS             <1..864
                     /locus_tag="PKO06_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV70294.1"
                     /translation="LAARALHALSPRAAEPFVRVNCASIPETLFESELFGHERGAFTD
                     ARERRIGRFELAHQGTLFLDEVGEVPIHLQAKLLRVLQDKEISRLGGSDSIAVDVRII
                     AATNRNLQKLVSEGRFREDLLYRLSVIQIGMPSLRERVSDIPILARHLLQRLAIDLGR
                     PPVALVDEAESALCRHSWPGNVRELRNVLERALVLSDGTTLDPTHFPFHQETASATHA
                     SSAPSLGSFPENGLIQGLEMMEREWLRQTLAACDGNRTKAAELLRISRPNLSFRLKKL
                     GLESETKKPGT"
     gene            complement(912..1532)
                     /locus_tag="PKO06_11400"
     CDS             complement(912..1532)
                     /locus_tag="PKO06_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70295.1"
                     /translation="MLKKALVILTIGLMIPAGVLLAGPGKGKGQANKKVNQELKAERK
                     AFKEQQKKENQAFKESLKNLPEDQRKAKMKEHREQQKAENKEFNQKMHEKRMAALKER
                     LATNTKLTDAQKQDLIAFFEAQYQDSVAFREKQQAAHMAEFEKIAADEKLTPEQKKAA
                     FKEYMDKQKAEFKEFQAKKKEARQDKKEELKPASATVDPNAAGTTK"
     gene            complement(1549..2136)
                     /locus_tag="PKO06_11405"
     CDS             complement(1549..2136)
                     /locus_tag="PKO06_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70296.1"
                     /translation="MRKLVVVLAVFLTVLCVPVWAETEGSDKDDLDLSPAAGGAKKQI
                     SADSIISKIKERKEKLLARIRQRFERMPERMRKMEEKVDKMSNRVSKAPKGDGEQGGV
                     DRKAQMKAKITERYEKFKGMISERKNKLGTKFDERKAKFQEKIGGKLKAEDMSKVMAE
                     FESAQKEVVAEVEKLLTEAQKKLDETYQKLLTKLN"
     gene            complement(2374..>3085)
                     /locus_tag="PKO06_11410"
     CDS             complement(2374..>3085)
                     /locus_tag="PKO06_11410"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV70297.1"
                     /translation="LKSVKNDDELAAVMAHELAHINKKHGIRQAEKAGLLTAIVLAMG
                     LKDETQKYQKYAAVAAFFANMKFSRNDEFEADKIAVQYASKAGYNPYGMSGFLQTINK
                     DNALTKVTKYFSTHPPTTERITRANAEAQKVSGRAPPANTTAQGTQTSPVTTGSSSGS
                     GSSSTGNSGTRPTTQPTTVPQGITLQEAYQDYLYTKSIYEQKVSQGAPYNEIMAAFND
                     YQRAKEIYFQLKNQAAQR"
BASE COUNT          576 a          908 c          760 g          841 t
ORIGIN      
        1 ctggcagccc gcgcccttca tgccctttcg ccacgcgccg ccgaaccgtt cgttcgcgtc
       61 aactgcgcct ctattcccga gacactcttc gagtccgagt tgttcggtca cgagaggggc
      121 gcttttaccg acgcccgcga acgtcgaatc ggacgtttcg aactggccca tcaaggaacc
      181 ctgtttctcg atgaagtcgg agaagtaccc atccacttgc aggcaaaact gcttcgagtg
      241 ctgcaagaca aggaaatatc ccgtctcggc ggatctgatt ccatcgcggt cgatgtgaga
      301 atcatcgccg cgaccaatcg aaatcttcag aaactcgtgt cagagggtcg ctttcgcgaa
      361 gaccttttgt accgattgtc tgtcatacaa attggcatgc catcgttgcg agaacgagtg
      421 tcagacatcc cgattctggc cagacatctg ctgcagcgac tggcaatcga ccttggtcga
      481 ccaccggttg cactcgttga tgaggcggaa agcgctctct gccgtcattc gtggcccgga
      541 aacgtccgcg aactcagaaa tgttctcgaa cgcgcactgg tcctctctga tggcaccacc
      601 ctcgacccga ctcactttcc cttccaccag gaaacagctt ctgcgactca cgcgtcttca
      661 gccccctccc tcggttcttt ccctgaaaac ggcctcatcc agggactcga gatgatggag
      721 cgcgaatggc tgcgacaaac gctcgcagcc tgcgacggca atcggacaaa agcggccgag
      781 cttctgcgaa tatcgcgacc caatctgagt ttcaggttga agaaactcgg actcgaatcc
      841 gaaacaaaaa aaccggggac ctgagtcccc ggccttttca gaataatcgt agcgtcttcg
      901 tcgacgtctg tttacttggt tgtccccgcg gcattcggat cgaccgtggc ggatgcaggc
      961 ttcagttctt cctttttgtc ttggcgcgct tccttcttct tggcctggaa ttccttgaat
     1021 tccgctttct gcttgtccat gtattctttg aatgcggctt ttttctgttc cggggtcagc
     1081 ttctcgtcag cggcaatctt ctcgaattcg gccatgtgag ccgcctgctg cttttcgcgg
     1141 aacgcgacac tgtcctgata ctgagcttcg aagaaagcga tcagatcctg cttctgcgcg
     1201 tcggtcagtt tggtgttggt cgccagacgt tctttcagcg ctgccatgcg cttctcgtgc
     1261 atcttctgat tgaattcctt gttttcagcc ttctgctgtt cgcgatgttc cttcatcttc
     1321 gcctttcgtt gatcttcagg cagattcttg agcgattcct tgaaggcctg gttttctttc
     1381 ttctgctgtt ccttgaacgc ttttcgttct gctttcagtt cctgattcac tttcttgttc
     1441 gcctggcctt ttcctttgcc agggcctgcc agcaatacac cggccggaat catcaacccg
     1501 atggtcaaaa tgacgagtgc ctttttcaac atgatctcga ctcccttgtc agttaagttt
     1561 ggtcagcagc ttctgatagg tctcatcaag ctttttctgc gcttcggtca acagtttttc
     1621 cacctcggcc acgacttctt tctgagcgga ttcaaactca gccatgacct tggacatatc
     1681 ttcagccttc agtttaccac caatcttttc ctgaaacttc gcttttcgct catcgaactt
     1741 ggtgcccagt ttgtttttcc gctcgctgat catgcccttg aacttctcat accgttcggt
     1801 aatcttggct ttcatctgag ctttccggtc gacgccaccc tgttcaccat ctcccttcgg
     1861 agccttcgac acgcggttgg acatcttgtc gaccttctct tccattttcc gcatgcgctc
     1921 cggcatgcgc tcaaaccgct gtcgaatccg cgccagcagc ttttctttcc gctctttgat
     1981 cttcgagatg atcgaatcgg ctgagatctg cttcttcgct ccaccagccg ccggggagag
     2041 atccagatcg tccttgtcag acccttcggt ttccgcccag accggcacgc acagcaccgt
     2101 cagaaaaacc gccaacacga cgacgagctt tctcatatac cgcctcctgc agttgaattt
     2161 cggaacaccg atagctatgc acaaaagatg ccaatgaatg gaatagcccg cacacgcgct
     2221 ctcacaaagg atgtgggcag aacaacgcgt ggacgtggcg aggttaatcg tccaaatatg
     2281 gacagttccg cttctgtttc gtccagattc ggacaacgtt gccgaaaaca aaaccggggc
     2341 acctcgaaag gtaccccggc tgtcggcacg tgatcagcgt tgtgcggcct ggtttttcag
     2401 ctgaaagtag atttccttgg cgcgctggta atcgttgaag gcggccatga tttcgttgta
     2461 gggcgctccc tgactgacct tctgttcata gatcgacttc gtatacagat agtcttgata
     2521 cgcttcctgc agcgtgatgc cctgcggaac agttgtgggc tgggtggttg ggcgagtgcc
     2581 cgagttccct gtcgagctgg agccgcttcc ggaagaggag cccgtggtca ccggactcgt
     2641 ctgcgttccc tgagccgtgg tgtttgccgg aggcgcgcgt ccggaaacct tctgcgcctc
     2701 agcattggcg cgggtaatgc gttccgtcgt cggcggatgc gtcgaaaagt atttcgttac
     2761 cttggttaaa gcgttgtctt tattgatcgt ctgcaggaag ccggacatgc cgtacgggtt
     2821 gtatccagct tttgaggcat actgcaccgc gatcttgtct gcctcaaact catcgttacg
     2881 cgaaaacttc atgttggcaa agaaggccgc tactgccgca tatttctggt acttttgcgt
     2941 ttcgtctttc agacccatgg ccagcacgat ggcggtcagc aaccccgctt tctccgcttg
     3001 acgaattccg tgcttcttgt tgatgtgtgc cagttcgtgg gccatgaccg cagcgagttc
     3061 atcatcgttc ttgacgctct tcaac
//
LOCUS       DAORHD010000519         3084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36329_length_3084_cov_3.489601, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000519 DAORHD010000000
VERSION     DAORHD010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36329_length_3084_cov_3.489601"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="PKO06_11415"
     CDS             <1..1338
                     /locus_tag="PKO06_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70298.1"
                     /translation="AELAVDPGQSSAKLRLLDDRPRKIQVQAMLPGGDTLAAEGEVLL
                     QAQAVQVEVTAQSPSVPRPKLWNGEVMVELGTDIAVGEKIPVQAVVTPELPDQKLRYL
                     WRADVGTRILEQKGDRVVVTRAETGEGTISVDVQSETGAFLGTGEIRFCVAVDSDLLQ
                     ATDSQKAAAARAALAEKFWKQGLGREAQEHLQQAINAGGSVENLQRLYDQRAGIIAEL
                     DGLLTVAETQIESDVKAAEDSLRQATARYPASVGIERLREKIRRHRLTVEGQARELLE
                     EVVRLLDQGRFQEALDVLTTLQPNRPLLPEAVQENMKSLEERAQKGLLNQVALVAKLA
                     ELSSNVDVSDPEALYRELSDLEKCVIASPSIIADSGMRQLNDALRQVGEKRARLSAHR
                     ETLTGITAAVHAGHVRGELIDEGLRVADEILAILPQDAPTLVARETLSKARGD"
     gene            1507..>3084
                     /locus_tag="PKO06_11420"
     CDS             1507..>3084
                     /locus_tag="PKO06_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV70299.1"
                     /translation="MNRSYALRGVLLAWLVLCLVSPLSAWQRMQVAGSFNGWNPTADA
                     GRMTADSEGYEARVFLHTGLVEYKFAANGSWQQNWGDDRDLEVLPQSGTALAGAGNIV
                     ARIERAGQYVFRFQPETGSYSLKLDPAAPVPPPVPEPPPPPAPVPPPTGFDFREETIY
                     FLITARFYDGDPENNFYCRDRIKKGDPHWRGDFKGLIAKLDYIKDLGFTAIWITPPIE
                     NRSGLDYHGYHGYDWNRIDPRNESPGATYKDLIDACHARGIKVIQDVVINHSSNYGIR
                     GQVWVDRLPIKYFRKAGMKLDWPYTGNLGNYQHPFREDNDNPLAPDWFRERQNSDPDG
                     TVPLKDPLTGAMLPTEKCDPNRFFGTDVMKLPGDWYHLHGFMSGGDWESPFSLQQKHM
                     AGDCLDLATERQNVRDYLNTAICRWLDLGVDAIRLDTVKHVERSDLLSYVNTWKKHKP
                     GLFVFGENLVKGHGWGDLGGDNGPSPIRPWWYTRLGNDPRDPRSGGDSGFSVLDFSLF
                     STFRDNLSKVSLGGIESV"
BASE COUNT          603 a          900 c          963 g          618 t
ORIGIN      
        1 gccgagctcg ctgtcgatcc gggacaatcg tcagcgaaac tgcggcttct cgatgatcgc
       61 ccccgaaaga tccaggtgca ggccatgctt ccgggaggag acacgcttgc cgcggaaggt
      121 gaagtgctgc ttcaggccca ggcggtgcag gttgaggtta cggcacagag tccgagcgtg
      181 ccacgcccaa aactgtggaa tggagaggtt atggttgagc ttggaactga catcgccgtc
      241 ggcgagaaaa ttcccgtgca ggcggttgtc acacctgagc tgcctgacca gaagctccgc
      301 tatctctggc gggctgacgt cggaacccga atcctggagc agaagggcga cagggtcgtg
      361 gtcactcgtg ccgagaccgg cgaaggaacc atttctgttg acgtgcagtc tgagaccggc
      421 gcgtttcttg gaactggaga gattcgtttt tgcgttgcgg tggattcgga tctcctgcag
      481 gccacggaca gtcagaaggc cgccgccgcc cgcgccgccc tcgcggaaaa gttctggaag
      541 cagggcctgg gtcgtgaagc tcaggaacat cttcagcagg ccatcaacgc gggcggttcg
      601 gtcgagaacc ttcagcgtct ctatgaccag cgtgcgggga taatcgctga gttggacgga
      661 ctgctgaccg tcgctgagac tcagatcgag tccgatgtga aagcggcaga agactctttg
      721 agacaggcca cggcgagata tcccgcctct gtcggtatcg agcgcctgcg tgaaaagatc
      781 cgacgccatc gcctgactgt cgaggggcag gcccgtgaac tcctcgaaga ggttgtgcgc
      841 ctgttggacc agggacgctt tcaggaagcg ttggatgtgc tgaccacact tcagccgaac
      901 aggccgcttc tgccggaggc tgttcaggag aacatgaaat cgcttgaaga gcgagcgcag
      961 aaaggtctgc tgaatcaggt ggccttggtc gcgaagctgg ccgagctctc gtcgaacgtc
     1021 gacgtctctg acccggaagc cctgtaccgc gaactgtccg atctggaaaa gtgtgttatc
     1081 gcctcgccaa gcatcattgc cgacagtggc atgcgacagc tgaatgacgc cttgcgccag
     1141 gttggcgaga agcgggctcg gctttcggcg caccgcgaga cgctgaccgg cattacggcc
     1201 gcggttcatg cggggcacgt tcgtggcgaa ctgatcgatg agggcctgcg cgtcgccgac
     1261 gagattctcg ccattctgcc gcaggacgcc ccgactctgg tcgcccggga gacgctttca
     1321 aaggctcgcg gagactgagc gcgcgtcgtg gtactgacgt gtgtcggtcg tttgtggtat
     1381 ggtctcgatc gatccgggcg cgagtttttg cccgtccgca ctgttcttcc agccccggcc
     1441 tgaggcggtg cggtcacgaa agcgcgattt gaacccggcg ccatgatttt gccgatgggg
     1501 ggtctcgtga acagatcgta tgccctgagg ggcgttctgc ttgcctggtt ggtgttgtgc
     1561 cttgtctctc cgctttccgc ctggcagaga atgcaggtgg ccggcagttt caacggttgg
     1621 aacccgacgg cggatgccgg ccggatgacg gccgatagcg aaggttatga agcccgggtg
     1681 tttctgcata ccggtctggt cgagtacaag ttcgccgcca atggaagctg gcaacagaac
     1741 tggggtgacg acagagacct ggaagttctg ccgcagagcg gcacggctct tgccggcgca
     1801 gggaacatcg tcgcccgcat cgagagagcc gggcagtatg tgtttcgttt tcaacccgag
     1861 accggttcat acagtttgaa gctcgacccc gctgcgccgg tacctccgcc ggttcccgaa
     1921 cctccgccgc cgcccgcacc ggtgccgccg ccgaccggtt tcgatttccg cgaggagacg
     1981 atctactttc tgatcaccgc ccggttctac gacggtgacc cggagaacaa cttctactgc
     2041 cgcgatcgca tcaagaaggg cgacccgcac tggcgcggtg atttcaaagg tctgatcgcg
     2101 aaactcgatt acatcaaaga tctcggcttt accgcgatct ggatcacgcc gccgatcgag
     2161 aaccgcagcg gtctcgatta tcacggctat cacggctatg attggaaccg catcgatccg
     2221 cgcaacgagt cgccgggtgc gacctataaa gatctcatcg atgcctgcca tgcccgcggt
     2281 atcaaggtga ttcaggatgt cgtcatcaac cattcgagca actacggcat tcgcggtcag
     2341 gtctgggtcg atcgactgcc gattaagtac ttccgcaagg ccggcatgaa gctcgactgg
     2401 ccgtataccg gtaaccttgg caactatcag catccgttcc gcgaagataa tgacaatcct
     2461 ctggcacctg attggttcag ggaaagacag aacagcgacc cggacggaac cgtgccactc
     2521 aaagaccctc tgaccggcgc gatgctgccg accgagaagt gtgatccgaa tcggttcttc
     2581 ggcacggacg tcatgaaact gcccggcgac tggtaccatc tgcacggctt catgtcgggc
     2641 ggcgactggg aaagcccgtt ttcgcttcag cagaagcaca tggccggcga ctgtctcgat
     2701 ctcgccaccg agcgccagaa cgtgcgcgac tatctcaaca cggcgatctg ccgctggctc
     2761 gatctgggcg tcgacgccat tcgcctcgac accgtcaagc acgtcgagcg cagcgatctg
     2821 ttgtcctacg tgaacacctg gaagaaacac aagccgggac tgttcgtgtt cggcgagaat
     2881 ctcgtgaaag gtcacgggtg gggcgatctc ggtggtgaca acggcccgtc gccgattcgg
     2941 ccctggtggt atacgcgcct gggaaatgat cctcgagacc cgcgatcagg aggagattcc
     3001 ggcttttccg tcctggactt ttccctgttc tccacctttc gtgacaatct cagcaaggtg
     3061 tctctcggcg gcatcgaatc agtt
//
LOCUS       DAORHD010000520         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36399_length_3080_cov_3.593058, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000520 DAORHD010000000
VERSION     DAORHD010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36399_length_3080_cov_3.593058"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKO06_11425"
     CDS             <1..209
                     /locus_tag="PKO06_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70300.1"
                     /translation="GTGLGKVDPGAIETIGFGYHEEYSVEWKVPVTVPTEAHSVTHAA
                     SETRRFRCLSFGDREYVVTLAWPE"
     gene            255..1649
                     /locus_tag="PKO06_11430"
     CDS             255..1649
                     /locus_tag="PKO06_11430"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV70301.1"
                     /translation="MLLKRPVRDVSTFSRVDRALILLTLPALNDSERQQLSGLVRSLS
                     IVEWEEAREKIDLNDIGPVVWIMLGRADLRGVVPPQLVRHLEARYEEIRRRNEARRLL
                     AGKLFHRFQEIGIEAIVLKGMLFAERIYGEPAYKKMNDLDLLVRFRDIARVKQIFAEL
                     ELVPLKLLEGGEDEQPDEHRNYHLPSYVSRDGSFVVGTHWSLCSPKRGYAIDIEALWN
                     RSEAARVADQSVLSLSAIDALHHLVMHFHYYKTGLKELGDFAAWIRYWAPFDWQAFAA
                     EVERAGTWSPAFRTLTLVETLYRVGVPADFLQRCRERTDRATRCDTVKQSARCELLLE
                     SRSTWAAEIEKAWMQFSFAGRWYDKPRHFLTFWQRLLFPPPATLLRVNFCVAGEKPWW
                     WLWMMNLWRTAREVGRSFGLAIFVLLMLKSTWELLSDLACECVGRPRDRLADLCRELG
                     TDESQLKRMMHSMD"
     gene            complement(1683..1910)
                     /locus_tag="PKO06_11435"
     CDS             complement(1683..1910)
                     /locus_tag="PKO06_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70302.1"
                     /translation="MNLSRLVLSFVLAGVLLYILFPAIYCLPLLFIYSKPLPQSVVDS
                     IVVFTSPLEPFYDRIRIYRQFHLWQWDLFFR"
     gene            complement(1965..2192)
                     /locus_tag="PKO06_11440"
     CDS             complement(1965..2192)
                     /locus_tag="PKO06_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70303.1"
                     /translation="MKTYQAGIAVLILLLMLYFLCPVTFFIALKHLYGMPFPNDLERK
                     LDVVFGPVQFLADHIPWYGSFLRWQADTFLL"
     gene            2406..>3080
                     /locus_tag="PKO06_11445"
     CDS             2406..>3080
                     /locus_tag="PKO06_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70304.1"
                     /translation="MIKRVPERKITGRRQTDARGQGVCHAAILSIRTFFLLVFVMLLA
                     NQLEAGLKPPIMLEREENNTQVPFEPWRERSTQAFTYVHEQPWLTNEFKLKHTTFKDV
                     PGYRQQLTTIQQASAYGHNMTSPLEFQAGRHWTGNSTYRPVDGVTLYYPWGNRLSTTV
                     TTGRFAYIYEHQSIRRPDFFEGQIHYQFNDQTFLRVQGDQEVDGQNTTVQVGFAIDSL
                     KLIGESR"
BASE COUNT          686 a          833 c          916 g          645 t
ORIGIN      
        1 cgggaaccgg attgggaaag gtcgaccccg gcgcgatcga gacgatcggc ttcggctatc
       61 atgaagaata ctcggttgaa tggaaagtcc cagtcaccgt gccaaccgaa gcgcattcgg
      121 tgactcacgc ggcaagcgaa acacggcgat tccggtgtct gtcgttcggt gaccgtgagt
      181 atgtcgtgac gctggcctgg ccggaataac ccgccgtatc tgcgtgaacc cactcgaatc
      241 ctgaggagac cagcatgctg ttgaaacgtc ccgtgcgaga tgtgtcgacg ttttcgcgtg
      301 tcgaccgtgc cttgattctc ttgaccctgc cggcactcaa cgactcggaa cggcagcaat
      361 tgtccgggtt ggtacgatct ctcagcattg ttgaatggga agaagcacgc gagaagatcg
      421 atctgaacga tatcggacct gtcgtctgga tcatgctggg ccgtgccgat cttcgagggg
      481 tggtgccccc acagctcgtt cgccatctcg aggcgcgata cgaagagatt cgacggcgaa
      541 acgaggcccg acgtctcctg gccggtaaac tgtttcaccg gtttcaggaa atcggcatcg
      601 aggcaatcgt gttgaagggg atgctctttg ccgaacgtat ctacggcgag cccgcctaca
      661 aaaagatgaa cgacctcgac ctgctggtac ggtttcgtga tatcgcacgg gtgaaacaga
      721 tcttcgcgga actcgaactg gtgccgctca aactgctcga gggcggagaa gacgaacagc
      781 ccgacgagca ccgaaactac catcttcctt cctacgtctc gcgtgacggc agtttcgttg
      841 tcggcacgca ctggtcgctc tgttctccga aacgaggcta tgcgatcgat atcgaagctc
      901 tctggaatag atcagaagca gctcgtgtcg ccgatcagtc agtactatcg ctgagcgcga
      961 tcgatgccct gcatcatctg gtgatgcatt ttcattacta caagaccggg ttgaaggaac
     1021 tcggtgattt tgccgcctgg attcgctact gggctccgtt cgactggcag gcgtttgccg
     1081 cggaagtcga gcgggccgga acgtggagcc cggcctttcg cacgctgacg ctggtcgaga
     1141 cattgtatcg cgtcggggtg cctgccgact ttctgcaacg ctgccgcgaa cgcactgatc
     1201 gcgccacgcg atgtgacacc gtgaagcagt cggctcggtg cgagctgctg ctggagagcc
     1261 gcagcacctg ggctgccgaa atcgagaaag cctggatgca gttttccttt gctggtcgct
     1321 ggtatgacaa gccgcgacat tttctcacct tctggcaacg gctgctgttt ccgccgcctg
     1381 cgacgcttct gcgcgtgaat ttctgtgtcg cgggcgagaa accgtggtgg tggctgtgga
     1441 tgatgaattt gtggcgcact gccagagagg ttggccgcag tttcggattg gcgatctttg
     1501 ttctgctgat gctcaagagc acctgggaac tgctatcgga tctcgcctgc gaatgtgtcg
     1561 gcagacctcg cgatcgtctg gccgatctct gccgtgaact tggcaccgac gagtcccagt
     1621 tgaagagaat gatgcattcg atggactgac cggctgcgct tcggaggggt ggcccacctc
     1681 ggctaccgga agaagagatc ccattgccag agatgaaact gacgatagat acggattctg
     1741 tcgtagaacg gctcgagagg agaggtgaaa accacgatcg aatcgacgac cgattggggc
     1801 aatggcttgc tgtagatgaa cagcagtggc agacaataga tcgccgggaa caagatgtag
     1861 agcagcactc cggccagaac gaatgaaagg accagacggg agagattcat gggcgggctc
     1921 gcaatgcgca actgccgggc ggtgttgtca taagagtggg tctttcacag gagaaaggtg
     1981 tctgcctgcc atcgaagaaa actgccatac caagggatgt ggtctgccag aaactgcact
     2041 ggcccaaaga ccacatcaag ttttctttca aggtcgtttg gaaatggcat gccatacagg
     2101 tgcttgagtg cgatgaaaaa ggtgactggg cagagaaaat acagcatgag cagcagaatg
     2161 aggacagcaa tgccagcctg ataggtcttc atcgaatttc cttcgctcag gcagtgtaac
     2221 agatccttcc ggccgtgtct tgccaaagtg agtcagccac agtgtttccg gtcccccgaa
     2281 tacggcaagt aagatactgt cgccgaaaca agctcctcac gattgggccg gagaattccg
     2341 atacaggaag atatgctgcg gcgatttcgc ccggttgatc ttcaggcgca cggccctccg
     2401 gacccatgat aaagcgggta ccagagcgga agatcaccgg aaggaggcag acggatgccc
     2461 gtgggcaggg tgtctgtcat gcggctattc tcagcattcg caccttcttc ctgctggtgt
     2521 tcgtgatgct tctggcgaac caactcgagg ctggtttgaa gcccccgatc atgctcgaac
     2581 gggaagaaaa caacacgcag gtgcctttcg aaccttggcg cgagcgttca acgcaggcgt
     2641 tcacctacgt gcatgaacaa ccctggctga ccaatgagtt caagctgaag cacacgacct
     2701 tcaaggatgt tccgggatac cgacagcaac tgaccacgat ccaacaggcg tcggcctatg
     2761 ggcacaacat gacttcgccg ctggagtttc aggctggtcg gcactggacc ggcaactcga
     2821 cctatcggcc ggtcgacggc gtgacactct actacccatg gggaaaccga ctttcgacca
     2881 ccgtgaccac cggtcggttc gcctacattt atgagcatca gagcatacgt cgccccgact
     2941 tcttcgaggg gcagattcat tatcagttca atgaccagac gtttctgcgc gtgcagggcg
     3001 atcaggaagt cgatggccag aacacgacgg tccaggttgg cttcgctatc gactcactca
     3061 aactgattgg cgagagccgc
//
LOCUS       DAORHD010000521         3077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36462_length_3077_cov_2.999338, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000521 DAORHD010000000
VERSION     DAORHD010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36462_length_3077_cov_2.999338"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..776)
                     /locus_tag="PKO06_11450"
     CDS             complement(195..776)
                     /locus_tag="PKO06_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNV70305.1"
                     /translation="MPRVVILMAQGFEEMELVITADLLRRAGVEVLLTSIEGSLEPIT
                     GSRGIRLLPDQTLQGVAPDSIDLLVLPGGLEGTRRLADSEQVLDLLRQVHRLERPVAA
                     ICAAPAVLVKAGIVTNRRVTCHPGAIEHMVGVVFQNHRVVVDGPITTSRAAGTTFEFA
                     FQLIDLLCGKQKVKEVNRGVLALIPDQLPDLMS"
     gene            complement(797..1555)
                     /locus_tag="PKO06_11455"
     CDS             complement(797..1555)
                     /locus_tag="PKO06_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HNV70306.1"
                     /translation="MSESKLPANFSQRVVAFLPTYNEAGNIKKIIEALLDLGPTVSVL
                     VIDDQSPDGTGDIVADIARTNPRVLLISRPPPRGRGLAGRDGFIWFQQHPEFDILVEM
                     DADFSHHPSAIPSLIAGLDEADVVIGSRYIAGGGEVGRPSDRKWISSLANAYLRFVLR
                     TRQRDCTSGFRVFKRSAFAGLDFSRFVSVGPPIVTELLFDLLRHRRRIVERPIIFADR
                     VEGTSKLTTRILIDSLWFPLWLRLRRHLRSRAEN"
     gene            complement(1548..2714)
                     /locus_tag="PKO06_11460"
     CDS             complement(1548..2714)
                     /locus_tag="PKO06_11460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV70307.1"
                     /translation="MTEAFRPARSVFHDFDCRHFRGDKPCAKAHGKACGPLLSEHSPC
                     NSYQTLGHRILVLKLGALGDVLRTTSLIPALRQRYPDCAIAWITKPAAMPLLSEAGLW
                     KVLPWDVDAELWTRNLDWDIVISLDKEEGPTSLATQLTARHAYGFGRNRHGLLTPLSA
                     STEYLFQLGLDDEEKFHRNTRPYPQLIAEACELDWDPNPYFMPLSESERTWADHASKG
                     WGQGPFVGLNIGAGEAFAGKQWPMERFVELARRLHDSGITPVFLGGEKEQAKYEQVQS
                     HLKAPGIYPGCRFTLRQFAALVSRCQILVAGDTFAMHIGIATGSYCVTLFGSTTAREI
                     EFYGNGQALIGQAPCAPCYRRVCPTHEECLQSISVDQVIQAVHQGLAAREKRRV"
     gene            complement(2744..>3077)
                     /locus_tag="PKO06_11465"
     CDS             complement(2744..>3077)
                     /locus_tag="PKO06_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70308.1"
                     /translation="FFGQGAPFAALIGFYGSLPPERVGEVNGVGNWKFWWRHRSLATG
                     TPAVFVDTGKFSEAVPWGRQYFGQVLASETHPIVSRGREIRQIIFTPLADYRGGLRYD
                     MSGLVREP"
BASE COUNT          676 a          848 c          961 g          592 t
ORIGIN      
        1 ccagaatgat caccgcgaac atgatctcga gaagggtgat tccactcttg cgtgcctgaa
       61 gcagctctcg cctcattgtg tttctcttcc tcactgcatc accatagcat tcgccatgca
      121 gacggtcaat ctttccctga caggctcgat cgagtgtgga aggttgtcga cggaaagtct
      181 gcttgcgcat ggaatcagga catgagatcg gggagctggt ctggaatcag ggcgagaacg
      241 ccgcgattca cttctttcac tttctgcttg ccgcagagca ggtcgatcag ttgaaaggcg
      301 aactcaaagg tggttccggc tgcccgggat gtcgtgatgg gtccgtcgac gaccacccgg
      361 tgattctgaa aaaccacacc caccatgtgc tcgatggcac ccggatgaca cgtgacacgg
      421 cgattggtca caatgccggc tttgaccaga acggccggtg cggcacatat cgcggcgaca
      481 gggcgttcca gtcggtgtac ctgtcgtagc agatcgagaa cctgttcgct gtcggcgagg
      541 cgtctggtgc cttcaagccc gccgggcaat acgagcagat cgatggaatc aggggccacg
      601 ccctgaagag tctgatcagg cagcaggcga atgccgcgtg agcccgtgat cggttcgagg
      661 gacccttcga tactggttaa caagacttcg acaccagcgc ggcgcaggag atccgcggta
      721 atgaccagtt ccatctcttc gaacccctgg gccatgagaa tcaccacacg aggcatagca
      781 gactcctctg tttgtcctag ttttctgcgc gagaacgcag gtgacgacgc aagcgaagcc
      841 agagcggaaa ccacagactg tcgatcagga tgcgggtggt cagcttcgat gtaccttcaa
      901 cccggtcggc gaaaatgatc ggtcgctcga cgattcgtcg gcgatggcgc aacaggtcga
      961 acagaagctc ggtgacgatc gggggcccaa cggaaacgaa gcggctgaaa tcgagtcctg
     1021 cgaaagccga acgcttgaag acccggaatc cggaggtgca gtcacgttgc cgggttcgca
     1081 gcacgaagcg aagataggca ttggccagcg acgagatcca cttgcggtca ctcggtcggc
     1141 caacctcacc gccccccgcg atgtaccggg agccgatgac gacatcggcc tcgtcaagcc
     1201 cggcaatgag tgaaggaatg gcagacggat ggtgcgaaaa atccgcgtcc atttccacga
     1261 gaatatcgaa ttcggggtgt tgctgaaacc agatgaagcc gtcgcgaccg gccaagcctc
     1321 gtccgcgtgg cggtggccgc gaaatgagca ggacacgcgg gttggtgcga gcgatgtcgg
     1381 caacgatgtc accggttcca tcaggagatt gatcgtcgat gaccagtact gagactgtcg
     1441 gtccgagatc caacagggct tcgataatct tcttgatatt gccggcttcg ttgtaggtgg
     1501 gaagaaaggc gaccacccgc tgtgaaaagt tggcaggaag cttcgattca gacacggcgt
     1561 ttctcccggg cagccaatcc ctgatgaacc gcctgtatga cctgatcgac cgagatcgac
     1621 tgcagacatt cttcgtgggt cggacagacc cgtcgatagc agggggcgca cggagcctgc
     1681 ccaatcagag cctgcccgtt tccgtagaat tcgatttctc gcgccgtcgt cgaaccgaac
     1741 aacgtgacac agtacgatcc ggttgcaatt ccgatatgca tggcgaatgt gtctccggca
     1801 acgagaatct gacatcgcga aacgagcgcg gcaaactgcc gcaaggtgaa ccgacaacct
     1861 ggatagatgc ctggggcttt gaggtgggat tgtacctgtt cgtattttgc ctgttctttc
     1921 tcgccaccga gaaagacggg agtgattccg ctgtcgtgca agcgtcgggc aagttcgaca
     1981 aagcgctcca tcggccactg cttgccggca aaggcttcac cggcaccgat gttcagaccg
     2041 acaaacggac cttggcccca ccccttgctg gcgtgatcgg cccaggttcg ctcggattcc
     2101 gagaggggca tgaaatacgg gtttgggtcc cagtcgagtt cgcaggcttc ggcgatcaat
     2161 tgcgggtatg ggcgggtatt acggtgaaat ttctcttcat cgtcgagccc gagctgaaac
     2221 agatactcgg tggaagctga gaggggtgtg agcagcccgt ggcgattgcg accgaacccg
     2281 taggcgtgcc tggcggtcag ctgagtggca agagacgttg gtccctcttc cttgtcgagg
     2341 gagatgacga tgtcccagtc gagattgcgt gtccagagct cggcatctac atcccagggg
     2401 aggactttcc aaagcccggc ttcagagagc aggggcatcg ccgcgggttt ggtgatccag
     2461 gcgatcgcgc aatcgggata gcgctggcga agcgccggaa tcagggacgt tgtgcgcagt
     2521 acatcgccca gagcgccgag tttgaggacc aggatgcggt gtccgagagt ttgatacgag
     2581 ttacagggcg agtgttcgga aagcagcggc ccgcaagcct tgccatgagc tttggcacag
     2641 ggcttgtcac cgcgaaaatg tcgacagtcg aagtcgtgaa acacgctgcg ggctggacga
     2701 aaggcttcgg tcatgaagcg gatcctgaga gtgtgaagat aggttacggc tcgcgtacga
     2761 ggccggacat atcataccgc aatcctcccc gatagtcagc caacggggtg aaaatgatct
     2821 gtcggatctc gcgcccgcga ctgacgatgg gatgcgtttc cgaggcgagc acctgaccga
     2881 aatactggcg tccccagggc acggcttcag agaacttgcc ggtatcgacg aaaaccgccg
     2941 gggtgccggt tgccagtgac cggtggcgcc accagaactt ccagttgccg accccgttta
     3001 cttcgccgac ccgttccggg gggaggctgc cgtagaagcc gatgagggcc gcaaaaggag
     3061 ctccctgacc gaaaaag
//
LOCUS       DAORHD010000522         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36599_length_3069_cov_3.393829, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000522 DAORHD010000000
VERSION     DAORHD010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36599_length_3069_cov_3.393829"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /locus_tag="PKO06_11470"
     CDS             complement(<1..1296)
                     /locus_tag="PKO06_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70309.1"
                     /translation="MSDGCRTRPAAVVKFAGVLLALLLVTVVLAAGPRVDYQWVETNL
                     ALTANGHAIVTTKVRIAVLEGSMGGWDYQGVAGDPVFATEHCLVLFADRSSHPVTVKP
                     QANRLWAIDIADGTRIPAGTAATFVLTFTVNLLGSGHLGLSRAEKTGQEVIYLNWADP
                     AFDEPLEHRTVYVHFPHEVSDPTAVNPDEWRRTILTEPFMNEYYRIDYRAQRTDAGKL
                     FLTQVLHRESVGTRESMRVQQYLPRAAFPGFRLPESSPSTPQPQGVIEGAPPSSDIGE
                     SESETHRRRRELAESDPERSFSTSPWRARFAAGGTVLFITYLALFSLYVKRRRQLADL
                     PKLRELSWEGENWEPPRIQVASFRKNGKVADLAILEAMLLLELPPIKLIACMVAQLER
                     EGIVRVFAAKPLRLEILEKSRTGDPYEQLLLDAVDADGAV"
     gene            complement(1430..1822)
                     /locus_tag="PKO06_11475"
     CDS             complement(1430..1822)
                     /locus_tag="PKO06_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70310.1"
                     /translation="MFLPFGEIKENRLIVSFSSADYSVATVLNAVKERLDIFGEMKVT
                     FLGASTEIPSGPSPVFRPVAIQAFFEYQGSGDARPALEKTYALVWESVALTFPGEAAW
                     ATAKGDFGRFISSQAELLRARTDSTKGA"
     gene            complement(1894..2808)
                     /locus_tag="PKO06_11480"
     CDS             complement(1894..2808)
                     /locus_tag="PKO06_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNV70311.1"
                     /translation="MRTMMVCLLTLLFVPAVIVAFPLNIAHRGARSVAPENTMAAFQV
                     AVERFGADMVELDVHLSKDGVPIVIHDDDLVRTTNAEEVYPDRAPWLVADFTAAELLM
                     LDAGSWFVEKDPFGQIKAGAVPAADVAEYKSGRIRLPTLRTVLIWAARKGVRLNVEIK
                     DFPVFYPGIVEKIVTEIRRAKLEQNVIISSFNHQALVDMQKVAPEIETGALNEDPVYP
                     LKPYLVDLLQVKAYHPSFEVLGEKSVDYRRHGRMRTDIVQEAQQAGLKVNVWTVNDPA
                     KMKSLIEAGVDGIITDFPQTLRTLGGGK"
     gene            complement(2822..>3069)
                     /locus_tag="PKO06_11485"
     CDS             complement(2822..>3069)
                     /locus_tag="PKO06_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70312.1"
                     /translation="PVGFRCWKLGGSQCWVRWQDIEAVRPFWLPLGCSSLRVYFKGSA
                     GPVWLPVSLSEFEAFRLYAFQFLEDQNPLKEHLAKMK"
BASE COUNT          654 a          972 c          856 g          587 t
ORIGIN      
        1 caccgcgcca tcggcatcga cggcatcgag cagtagctgt tcatagggat cgccggtacg
       61 cgacttttcg agaatctcca gtcgcagcgg cttcgcggca aacacgcgca cgatgccctc
      121 gcgctcgagt tgggccacca tgcaggcgat cagcttgatc ggcggcagtt cgagcagcag
      181 catggcttcg agaatggcca ggtcggcaac cttgccattc ttgcgaaagc tggccacctg
      241 aatgcgcgga ggttcccagt tctcgccctc ccagctgagc tcgcgcagct tcggcagatc
      301 cgccagctga cgccgccgct tcacatacaa gctgaataac gccagatacg tgataaatag
      361 cactgttcca ccggcggcaa atcgggccct ccagggggac gtcgagaaac tccgttccgg
      421 atcagattct gccagctcgc gccgacgacg atgcgtttct gactcgctct caccgatgtc
      481 agatgagggc ggggcacctt cgatcacgcc ctgcggctgc ggcgtcgatg gtgaggattc
      541 cggcagacga aagcccggaa acgccgcacg cggtagatac tgctgcaccc gcatgctctc
      601 gcgcgtgccg accgactctc ggtgcagcac ctgagtcaga aacagctttc ccgcatcggt
      661 tctctgcgcc cggtaatcga tgcgatagta ctcgttcatg aacggctcgg tcagaatcgt
      721 gcgtctccat tcgtccgggt tcaccgccgt cggatcgctg acttcatgcg ggaagtgaac
      781 gtagacggtg cgatgttcca gcggttcatc gaaggccgga tcagcccagt tgagatagat
      841 gacctcctgc ccggtcttct ccgctcggct gagcccgaga tgcccggaac cgagcaggtt
      901 caccgtaaag gtcagcacga atgtcgcggc cgttcccgcc ggaattctgg tgccatccgc
      961 gatatcgatt gcccagagtc ggttggcctg gggtttgacg gtcaccggat gactgctgcg
     1021 gtccgcgaac agcaccagac aatgttcggt cgcgaacacg gggtccccgg ccactccctg
     1081 gtagtcccag ccgcccatgc tgccttcgag cacggcgatg cgcaccttgg tcgtcacgat
     1141 ggcatggccg ttggcggtca acgccaggtt ggtttcgacc cactggtagt cgacccgcgg
     1201 accggccgcg agaacgacag tgaccagaag cagagccagc agcacaccgg cgaatttcac
     1261 gaccgccgct gggcgagtgc ggcacccatc actcataacg ttcttccttt acccgtttca
     1321 acagttcagc atactgatca tcactcaaga gttctcgtcc tcaactcgga caatcagcag
     1381 aaaacgactg tcgacgcaag accgcaagct cgaacgtacg cgaaccggct caggctccct
     1441 tggtactgtc ggttcgagcc cgcagcagct cagcctgtga cgagatgaac cggccgaaat
     1501 ctcccttcgc cgtcgcccag gccgcctctc ccgggaaggt gagcgcgacc gactcccaca
     1561 ccagagcgta ggtcttttcg agcgcggggc gggcgtctcc cgagccctga tactcaaaga
     1621 acgcctgaat ggccacgggg cgaaacacgg gggacggccc ggaagggatc tcggtgctcg
     1681 cgccaagaaa ggtgactttc atttcgccga agatatcgag acgctccttg accgcgttca
     1741 acaccgtcgc cacggaatag tcggcggaac tgaacgagac gatcagacgg ttttccttga
     1801 tttcaccgaa aggcagaaac atgggattcc tccagatggt tgggtagggc ctctgcaaaa
     1861 gacttcactc tctgtcgaca gaataccaca actctacttg cccccgccca acgtgcgcag
     1921 ggtctgcgga aaatccgtga tgatgccgtc gacgccggcc tcgatgagac tcttcatctt
     1981 ggcgggatca ttcacggtcc agacgttcac cttcaacccc gcctgctggg cttcctgaac
     2041 gatgtcggtg cgcatacgtc catgacggcg atagtcgacg gacttttcgc ccagcacctc
     2101 gaagctcgga tgataggctt tgacctgcag cagatcgacc agatacggct tcagcggata
     2161 caccggatct tcgttcaaag ctccggtctc gatttccggc gccactttct gcatgtcgac
     2221 gagcgcttgg tggttgaatg aggagatgat gacattctgc tcgagcttcg cgcggcgaat
     2281 ctcggtcacg atcttctcga cgatgcccgg gtaaaacacg ggaaaatcct tgatctcgac
     2341 gttcaggcgc actcccttgc gagccgccca gatcagcacg gtgcgcagcg tcggcaggcg
     2401 aatgcgaccc gacttgtact ccgcgacatc cgccgccgga actgcgccgg ccttgatctg
     2461 accgaacggg tccttctcga caaaccacga gccggcatcc agcatcagca gctcggcagc
     2521 cgtaaaatca gccacgagcc agggagcgcg gtccggatac acctcttcgg cattcgtggt
     2581 gcgtaccagg tcatcgtcgt gaatgacgat cggaacgccg tctttcgaca ggtgtacatc
     2641 gagctcgacc atatcggcac cgaaccgttc aaccgctacc tgaaaagcgg ccatggtgtt
     2701 ttccggggct acgcttctcg ctccgcgatg tgcgatgttc agaggaaaag caacaataac
     2761 cgctgggaca aacaacagag tcaaaagaca aaccatcatc gtgcgcatct gaatctcctt
     2821 gtcacttcat tttagccaaa tgctctttga gcggattttg atcttcgaga aactgaaagg
     2881 catacaggcg aaatgcctcg aactcagaga gcgaaacagg cagccagaca ggccccgcac
     2941 tgcctttgaa atacactctc agactgctac acccgagagg cagccagaac ggtcgtacgg
     3001 cttcgatatc ctgccagcgc acccagcact gacttccacc gagcttccag caccgaaacc
     3061 ctaccgggc
//
LOCUS       DAORHD010000523         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36612_length_3069_cov_2.761115, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000523 DAORHD010000000
VERSION     DAORHD010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36612_length_3069_cov_2.761115"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKO06_11490"
     CDS             <1..643
                     /locus_tag="PKO06_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70313.1"
                     /translation="INGRSFYQIVGFCYNSTTSMTFHTRTSARIVFFPCLLLLAGCLL
                     IAGCDEGKGTSTPVQVLNPSISDLSVSGNLITRSAGGILTLACNWTNPDTVYSAAAYL
                     AFSTTLTDSSSGPTGVLASGTASSANLLATIRGGFNEEFATPLLIPTAISSTEREGYW
                     RVEIPLASGVTSDAPLGKNQMLLWMVINGRKSNTLAFELEFTGSSSASLRAEP"
     gene            complement(714..2006)
                     /locus_tag="PKO06_11495"
     CDS             complement(714..2006)
                     /locus_tag="PKO06_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase C-terminal beta-sheet
                     domain-containing protein"
                     /protein_id="HNV70314.1"
                     /translation="MRRFLSRVLPAMILVVFFLGADALLAAGSRAANSAILIQTFGWN
                     SQRSGSPCRWYKLIGERAEDLAELGVTHAWLPPVTRSVSPQGYLPTDYYDLGSKESPT
                     FYGTLDDLKQCLSKLNAAGISPLADIVINHRCAGKQDENGIWNVYQFPSGKAAWEKWA
                     VVAGEYGGTGKADSGDNFQPAPDVDHANSQVQEDIIAWMNWLKTLGFKGWRYDFVKGF
                     APAYVGLYDSKTKPVFSVGELWTNMSYEGSTPVANQNPHRQQLCNWLDGAGKTVTVFD
                     FTTKGILQEAVNGDYWRLRDSEGKASGLIGWWPQRAVTFLDNHDTGSQQNHWPFPGQK
                     VMQGYAYILTHPGIPCLFWEHVYDWNLKAQIRKLTDIRRKFGIDADSKLEIVKAERGL
                     YAAVIDGKVAVKLGWVDWSPDPAFKLLASGDQFAIWGK"
     gene            complement(2115..>3069)
                     /locus_tag="PKO06_11500"
     CDS             complement(2115..>3069)
                     /locus_tag="PKO06_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70315.1"
                     /translation="TRLVGMVPPQVGTLERAVQVLGAIGPLAKAAVPELIVALSQPHP
                     PRNYSGERMSIDHNIHRSVIVALGDIGPDAASAIPCLVKLLEEEKKAFLTEPICQALA
                     SMSSAAAPAVPALVRQLSGSHAQRAIETLAALGPTANPAVAELEQLLETGDHGSRVSV
                     YKAISSIETDKAHRIAVACRYLRDPELAALALNTLAVLPDIGTSALAELLEELGKRNA
                     FDPQMMDVIRALGNTGPAAAEALPKLIKDNTNSSYSEPVKQAAFEAIKKIDPLGEKTM
                     ALVKPDLEDAFRVRGCVELLEFLATPACRELAQSTRQRWKF"
BASE COUNT          606 a          932 c          883 g          648 t
ORIGIN      
        1 aataaacggg aggtcatttt accagattgt cggattttgc tacaattcaa ccacttccat
       61 gacattccat acccgcacat ccgctcgaat cgtctttttc ccgtgtctgc tgctgttggc
      121 tggctgcctg ctgatcgccg ggtgcgacga aggcaagggc acgtcgacgc ctgttcaggt
      181 gctgaaccca tcgatcagcg atctgtcggt atcggggaac ctgatcacac gcagtgctgg
      241 cggcattctc acgctggcct gcaactggac gaatcctgat accgtctatt ccgcggccgc
      301 gtatctggcc ttttcgacga cactgaccga ttcgagcagt ggaccaaccg gagttctggc
      361 ctcaggcacg gcctcgtcag cgaaccttct ggcgacgatc cgcggcgggt tcaacgaaga
      421 gtttgcgacg cccctgctga ttccgaccgc gatcagcagc accgaacgcg agggatactg
      481 gcgggttgag attccgctgg cttccggcgt cacgtccgat gcgccactcg gcaagaatca
      541 gatgctgctc tggatggtga tcaacggccg caagagcaac accctggcat tcgaactgga
      601 gttcaccggg tcgtcgtcag caagcctgcg cgccgaaccc tagcctgttc gtcaaaaaac
      661 aacccgcccc agcgtgttga ggcgggttgt tttgtacaag ttatcgtcga gcgttacttg
      721 ccccagatgg caaactgatc accgctcgcc aacagtttga aagccgggtc aggcgaccaa
      781 tcgacccacc cgagtttcac ggccaccttg ccatcgatga cggctgcgta caagccgcgc
      841 tcggctttga cgatctccag cttgctgtct gcgtcgatgc cgaacttccg acgaatgtcg
      901 gtcagtttcc tgatctgcgc tttcaggttc cagtcgtaga cgtgttccca gaacaggcag
      961 gggatgccgg gatgcgtcag aatataggca tagccctgca tgactttctg cccggggaac
     1021 ggccagtgat tctgctgtga accggtatcg tggttgtcga gaaaggtcac ggcgcgctgc
     1081 ggccaccagc cgatcagacc ggatgccttg ccttcgctgt cacgcaagcg ccagtagtcg
     1141 ccgttcacgg cttcctggag aatgcccttg gtggtgaaat caaacaccgt caccgttttg
     1201 cccgctccgt cgagccagtt gcagagctgc tggcgatgcg ggttctgatt cgccaccggg
     1261 gtcgaacctt cgtaggacat gttggtccag agctcgccga ccgaaaacac cggcttggtc
     1321 ttgctgtcat acaagccgac atacgcgggg gcgaagccct tcacgaagtc atagcgccag
     1381 cctttgaagc cgagggtctt gagccagttc atccaggcga tgatgtcttc ctgcacctgg
     1441 ctgttggcat gatcgacgtc aggagccggc tgaaagttat cgcccgagtc ggctttgccg
     1501 gtgccgccgt attcgcccgc aaccacggcc catttttccc aggcggcttt gcccgaggga
     1561 aactgataca cattccagat gccgttttca tcctgcttgc cggcacagcg atgattgatg
     1621 acgatatcgg cgagcggact gatgccggcc gcgttgagtt tgctcagaca ctgtttcagg
     1681 tcatccagcg tgccgtaaaa ggtcggcgat tccttgctgc caagatcata gtaatcggtg
     1741 ggcagatacc cctgcgggct caccgagcgg gtgaccggcg gcagccacgc atgtgtgaca
     1801 cccaactcgg ccagatcttc ggcgcgttcg ccgatcaatt tgtaccagcg gcacggactg
     1861 ccgctgcgct ggctgttcca accgaaggtc tggatgagaa tcgcggagtt cgcagcgcgc
     1921 gaacccgccg ccagaagcgc gtcggcaccc agaaagaaca cgaccaggat cattgccggc
     1981 aggactcggc tgagaaaacg ccgcatgata tgcctcctga aacgcaagtg aatcttcttt
     2041 attgtagccg atctgtacca actccgtcaa aatcaagggt tctcaggcca gggcagatcc
     2101 cctctggctg aggttcagaa cttccagcgt tgacgggtgc tctgggccag ttcccggcag
     2161 gcaggtgtcg ccaggaactc gagcaactcg acacatcccc gcacccgaaa cgcatcctcg
     2221 aggtctggtt tgacgagagc catggttttc tcgcccagag gatcgatctt tttgatcgct
     2281 tcgaaagccg cctgcttcac cggttccgag taagagctgt tcgtattgtc cttgatcagt
     2341 ttcggcaagg cttcggcggc agccggtcca gtattgccaa gtgcccttat gacatccatc
     2401 atctgcggat caaaggcgtt tctcttgcca agttcttcca ggagttcggc cagagcgctg
     2461 gtcccgatgt caggcagcac agccagggtg ttcagcgcca atgccgccag ttcgggatcg
     2521 cgaagatatc gacaggccac cgcgatgcga tgcgccttgt cggtctcaat gctcgaaatg
     2581 gctttgtaga cagagacacg agacccatgg tctcccgttt ccagcaactg ttccagttct
     2641 gcgacggctg ggttcgcggt cggccccaga gccgcgagtg tctcgatcgc gcgttgtgca
     2701 tgcgaaccgg acaactggcg caccaatgcc ggaacagccg gcgctgccgc actggacatg
     2761 cttgccagcg cctgacagat cggttcagtc agaaacgcct tcttttcctc ttccaggagt
     2821 ttcaccagac agggaatggc agaagcggcg tccggaccga tatctccgag agcgacgatc
     2881 acggaacgat ggatgttgtg atcgatgctc atgcgctcgc cgctgtaatt ccgtggcgga
     2941 tgcggctgcg acaaggccac gatcagttcc ggaacggcag ctttcgccaa cgggccgatc
     3001 gcccccagca cctgcaccgc ccgttcgagc gtgcccacct gtggtgggac cataccaaca
     3061 agccgggtg
//
LOCUS       DAORHD010000524         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36730_length_3063_cov_3.201130, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000524 DAORHD010000000
VERSION     DAORHD010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36730_length_3063_cov_3.201130"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PKO06_11505"
     CDS             <1..544
                     /locus_tag="PKO06_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70316.1"
                     /translation="TSIDSGEEMELALQAAETRQVIGVMNVSGAADAVFRLLVNGVLA
                     TTLARSLRLVLVQRLVPRLCADCRVAKPASAAMLKSLGIDAALLNASGAADNGREAPC
                     FFQPRGCPACSGKGYRGLQLIVEALEMNDELQRLVHKPDVVRADFEIAAGRAGWMPFR
                     KKAFVYALQGVIRVEDAVMG"
     gene            596..1078
                     /locus_tag="PKO06_11510"
     CDS             596..1078
                     /locus_tag="PKO06_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70317.1"
                     /translation="MEQMTDIQKKLAEIDRMRDEIRTMEEQIGQLGDRVDKCGGNDDA
                     YRQIGEWDRHAAELRQPLTKLIAEVRASDPAAFGAWVERNRETLRQQILMILATARPE
                     AKTIEDAVMQITLDAKFSNDDLTVLRLAGYLDEWRDVLTGTRPFALHPPSWETKPKVS
                     "
     gene            1075..1758
                     /locus_tag="PKO06_11515"
     CDS             1075..1758
                     /locus_tag="PKO06_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70318.1"
                     /translation="MTDQSLSLAAKIYVVWAALAVVFAILVAGLNAETVTRLLTIAFL
                     VLQIVARKRLAACGRFLSPRWRFVLLGTVLASVVEGFHMISKPVFDSVRINAQMPLSQ
                     ALGFYAIDLLFTVPAYLVILTVIWRLITRYKLSLWEYIFLVALAQTLGDGGIFYFASA
                     PFMLPFLPYPMTNYHAMNIIPYLAVREELAPTERSFRTPLTLIVHIIATYLVCGSLIK
                     IAARPFGFE"
     gene            1819..2418
                     /locus_tag="PKO06_11520"
     CDS             1819..2418
                     /locus_tag="PKO06_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70319.1"
                     /translation="MVNIFNKLIGGTAQAERLGKEAIELLDGWQPNEALAKAQEALAI
                     DPDNGDALSVCGFVYSSQKKHREALQVYQRLQRLEPESSDHWQCAGDEQRDLGQLEEA
                     EASYRHGLTLSPTSLVFRCKLATTLRKLGRLEEAARMLEAALALAATATEGLYNLVLT
                     LAVFNRDDAMPAILDRLEQVDPDQAREARRNLEVASLLT"
     gene            2505..>3063
                     /locus_tag="PKO06_11525"
     CDS             2505..>3063
                     /locus_tag="PKO06_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70320.1"
                     /translation="MQSYARIVIPLLLIGCFVIPACAKKLGFEYSDGTQGPIEVVDLP
                     TPKSPSRVFGLGEAMAAGKDVLLFRQVRKPTALKHMERNFFFISKLMESYEPTVKVIE
                     LSNSVSYPQGDYYADLMDYFPANGYGVVKKEGDGYKLYSSKFGYSEEYDTFVLPVKES
                     IEQVLGPCPYDKKKLIKSTSCYEPYN"
BASE COUNT          653 a          823 c          945 g          642 t
ORIGIN      
        1 cacctcaatc gactctggcg aagagatgga actggccctg caggccgcgg agactcggca
       61 ggtgatcggt gtcatgaacg tcagcggcgc ggccgatgcc gtcttccgac tgctggtgaa
      121 cggggttctt gcgaccacgc tggcgcgctc actgcggctt gtgctggtgc agagactggt
      181 gccgcggttg tgcgccgatt gccgcgtggc aaagcccgcc agtgccgcca tgctgaagag
      241 cctgggtatc gatgccgcgc tcctgaacgc gagcggtgcg gccgataatg gacgcgaagc
      301 cccgtgtttc tttcagccgc gagggtgtcc ggcttgctcg ggcaaggggt atcgtggttt
      361 acagttgata gtcgaggctc tggaaatgaa tgacgagttg cagcgactcg tacacaagcc
      421 cgacgtggtg agggcagact tcgagatagc cgctgggcgc gcgggctgga tgccgtttcg
      481 gaaaaaggca ttcgtctacg ccctgcaagg cgtgatcaga gtcgaagacg ccgtcatggg
      541 ttgagtcagg ggctggtcgg ggtgaaaaga ctggggataa aggcggtcag acgatatgga
      601 acagatgaca gacattcaga agaaactggc agagattgat cggatgcgcg atgagattcg
      661 cacgatggaa gaacagatcg gtcagctggg tgaccgcgtc gataaatgtg gtggcaacga
      721 cgacgcctat cggcagatcg gggagtggga ccggcatgcc gccgaattgc gacaaccgct
      781 gacgaagctg attgccgaag tgcgagcgtc tgaccccgcg gcgttcggag cgtgggtcga
      841 gcgcaaccgc gaaaccttgc gccagcagat tctgatgata ctggcgaccg cccgtcccga
      901 ggcaaagacc attgaagacg ccgtgatgca gataactctc gatgcgaagt tctcaaacga
      961 tgatttgacg gtattgagac tcgccggcta cctggacgaa tggcgcgatg tgctcaccgg
     1021 aactcgtccg ttcgctctcc acccgccctc gtgggaaacc aaacccaaag tgtcttgaca
     1081 gaccagtctc tgagtctggc cgcaaagatc tatgtcgtct gggccgcttt ggcggttgtg
     1141 ttcgccattc ttgtggctgg actgaacgcg gagactgtga cgcggcttct gaccatcgcg
     1201 tttctggtgc tgcagattgt cgcgcgaaag cgactggccg cctgcggacg attcctctca
     1261 ccccgatggc gcttcgtgct gctgggcaca gttctggcgt cggtggtcga agggtttcac
     1321 atgatctcga agccggtgtt tgacagcgtt cgcatcaatg cgcagatgcc gctttcacag
     1381 gccctggggt tctacgccat cgatcttctg ttcaccgtac ctgcctatct tgtcattctc
     1441 accgtgatct ggcgtctcat cacccggtac aaactctcgc tctgggaata cattttcctg
     1501 gtcgcactcg cgcaaacgct gggcgatggc ggcatcttct atttcgcgag cgctccgttc
     1561 atgctgccct tcctcccgta cccgatgacc aactaccatg cgatgaacat cattccgtat
     1621 ctggcggtca gggaagaact cgcgccgacc gaacgctcat tccgcactcc gctcacgttg
     1681 atcgtccata tcatcgccac atacctcgtc tgcggttccc tgatcaaaat cgccgcccgc
     1741 cccttcggct tcgagtgatc aatggtacgt agccggagaa aaggaaacga tgtatgattg
     1801 tctccgtgaa aggattcaat ggtaaacatt ttcaacaagc tcatcggcgg tacggcgcag
     1861 gcggagcggc tggggaaaga ggcgattgag ctgctcgacg ggtggcagcc gaacgaggcg
     1921 ctggccaagg cgcaggaagc actcgcgatt gatccggaca atggagatgc cttgtcggtc
     1981 tgcgggtttg tctacagttc gcagaaaaag catcgcgagg cgctgcaggt gtatcagcgg
     2041 ttgcagagac tcgaacccga gtcgtcggat cattggcagt gcgcgggtga cgagcagcgg
     2101 gacctcggac aactggaaga agctgaagct tcataccggc atggtctgac gttatcaccc
     2161 acgtcactgg tgtttcggtg caagctggca acgacgttgc ggaaactggg tcgtctggaa
     2221 gaggcggcac ggatgctgga ggcagcgctg gctctcgcgg caaccgcgac cgagggattg
     2281 tacaatctgg tgctgactct ggcggtcttc aacagggacg atgccatgcc ggcgattctc
     2341 gaccgactcg aacaggttga cccggatcag gctcgtgaag cacgtcgcaa cctcgaggtt
     2401 gcttccctgc tgacctgaag acggaatgat gaagcgttgc ttctgccggc tcttgcctga
     2461 ggcaaggcca tagcgcagtt aaccgcggat ctacggagga tacgatgcag tcatacgcca
     2521 ggatcgtcat tcccttgctg ctgatcggtt gtttcgttat tccggcttgc gccaagaagc
     2581 tcggattcga atattcggac ggaacccagg gaccgatcga ggtggtcgat ctgccgaccc
     2641 caaaatcacc gagtcgcgta ttcggtctgg gtgaagccat ggcggcagga aaagatgtac
     2701 tgctgtttcg acaggttcgg aaacccacag ccttgaagca catggagcgc aacttcttct
     2761 tcatctcgaa actcatggaa tcctacgaac cgacggtgaa agtcatcgaa ctgtcgaaca
     2821 gtgtgagcta ccctcagggc gactactacg ccgatctcat ggactacttt ccggccaatg
     2881 gatatggcgt cgtgaagaag gagggtgacg gatacaagtt gtactcatcg aagtttgggt
     2941 acagcgaaga gtatgacacg tttgtgctgc cggtaaagga gagtatcgag caggtgctcg
     3001 gaccatgccc gtatgataaa aagaagctga tcaaaagtac gagttgctat gagccttaca
     3061 aca
//
LOCUS       DAORHD010000525         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36820_length_3058_cov_3.127206, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000525 DAORHD010000000
VERSION     DAORHD010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36820_length_3058_cov_3.127206"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="PKO06_11530"
     CDS             complement(<1..1717)
                     /locus_tag="PKO06_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70321.1"
                     /translation="MRGANRRGIILGIVVLTSAMLAVFLFAYNYTVRRQNIRAHFEQI
                     GEVGATLAKTGVNLLFEEVSSGFDTMILAAAPTLVGSVLTSNSLTIEDSDVSGSICAK
                     VRDDFDHYLSQLTNLSGGAYSHTPRCMKMEIAFENIQQLNPILPTDGVDEVRLRLQAG
                     RDAVEKSGEAVVSCTVEYQSLRRKAQIRRQFRVVSMVPGPFARFSLFVPYTPYANSYN
                     ALGVFFNGTIDGSYQHPHPTPQHFSGALKLINGTDSYIVGTMPDPKTDLQNRGWVFLG
                     PAYDPPTNATSAVILRIPSGYDASSGGHFQFSQPKVQNGLSIVNTEQIDTALYNTAPN
                     ATTTSVMLGGTFQGFYTNSHNAGVPTGVGAAGQGLWSDLDAIRPWECASSWIMPYGDR
                     NSPSRTLMIGPVLAGFLKYYFLKEGGSSPTWGFILKGLSETVYDPVTQVQAADKPTPT
                     ISPLYQELFKPTGGEAKTGFRNFLKVMPFNSVPHITTVPLAEGVAFNALFDFMEYNVG
                     HYPLYHEGSMVMQANVANEFYVPSFGRMTVPTPPASVKGIHPWDDVKIKFNETDGVIP
                     QDNLYF"
     gene            complement(1811..2608)
                     /locus_tag="PKO06_11535"
     CDS             complement(1811..2608)
                     /locus_tag="PKO06_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNV70322.1"
                     /translation="MSQQNPEIHVNIAFRYMRPEDFEFLGLQHLHPELYFSGDDADSV
                     TPERLDQIREEMNRRAFQPTFHAPFYDLNPGAHDAKVRQVAGDRYHWALDLGKKFGAR
                     HVILHPGYGPWVLKKDFGNWLTRARPVLDQVVEKARSLGLKIAFENIYDDSWNELAEL
                     IGCYPADIVGVCFDVGHFNIFSEKPMAKWLDVLGPRIFELHLHDNLGAEDLHIAIGDG
                     SVKFAPLIQWLRARDQMPVLGLEMEQKTHVIKSVSRLRQWFEEKGDS"
     gene            complement(2605..2811)
                     /locus_tag="PKO06_11540"
     CDS             complement(2605..2811)
                     /locus_tag="PKO06_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNV70323.1"
                     /translation="MPIKKDLLDILACPACHGTIVHDEAKDTLNCVSCRRRYPIRNDI
                     PVMLIDEATIPPEDNPSSGQGAPR"
BASE COUNT          706 a          858 c          833 g          661 t
ORIGIN      
        1 gaaagtagag attatcctgc ggaatcacgc catctgtctc gttgaacttg atcttcacat
       61 cgtcccacgg atgaatgccc ttcacgctcg ccggtggggt tggtacggtc atccggccga
      121 acgacggaac gtaaaactcg ttggccacat tggcctgcat gaccatgctt ccttcgtgat
      181 acagcggata atgaccgaca ttgtattcca tgaaatcgaa cagggcgtta aacgccactc
      241 cctcagccag cggcacggtc gtgatatgcg gcaccgagtt gaagggcatc accttcagga
      301 agttccgaaa gcccgttttc gcttctccac ccgtcggttt gaagagttcc tgatacaggg
      361 gtgagatcgt cggagtcggt ttgtcggccg cctgaacctg agtgaccgga tcatagaccg
      421 tttcagagag ccccttgaga atgaagcccc aggtggggct cgacccaccc tctttcagaa
      481 aatagtattt gagaaagccc gccagcacag gccctatcat cagggttcgc gagggagaat
      541 tgcgatcgcc gtaaggcatg atccaggagg aggcgcactc ccagggccga atggcatcga
      601 ggtcgctcca caagccctgg ccggccgccc cgactccggt cggtacgcca gcattatgcg
      661 agttggtata aaatccctga aacgttccac ccagcatcac cgaggttgtc gtggcattcg
      721 gagccgtgtt gtaaagcgct gtatcaatct gctccgtgtt cacgatcgac agtccgttct
      781 gcaccttcgg ctgagaaaac tgaaagtgcc cgccggaact ggcatcgtat ccggaaggaa
      841 tgcgaagaat gaccgcactg gtcgcatttg tcggcggatc atatgccggg ccgagaaaca
      901 cccagccacg attctgcaaa tcggttttcg gatcgggcat ggtcccgaca atgtatgaat
      961 cagtgccatt gatcagcttc agggcccccg aaaaatgttg gggagttgga tgtggatgct
     1021 gataggaccc atcgatcgtc ccattgaaaa acacacccag cgcattatag gagttcgcat
     1081 acggcgtata ggggacaaac agcgaaaaac gcgcgaacgg acccggtacc atactgacaa
     1141 cgcgaaactg gcgcctgatc tgagcctttc gcctcaaaga ctggtactct accgtacacg
     1201 acaccacggc ctcccccgac ttttccacgg catcacgacc tgcctgcaat cgcaagcgca
     1261 cttcgtcgac gccatcagtc ggcagaatgg ggttgagctg ctgaatattc tcgaaagcga
     1321 tctccatctt catgcagcga ggggtgtgag aatacgcgcc acctgagaga ttggtgagct
     1381 gcgacagata gtggtcgaaa tcgtctcgta ccttggcgca gatgctgccc gatacatcgg
     1441 agtcttcgat cgtaagcgag ttgctcgtca gaacgctgcc aaccagggtt ggcgcggctg
     1501 ccaggatcat cgtgtcgaag ccggaactca cttcttcgaa gagaaggttg actcccgttt
     1561 tcgccagagt cgcgccgact tcgccgatct gttcgaagtg cgcacggata ttctgccgac
     1621 gcaccgtgta gttgtaggca aaaagaaata ctgccagcat cgccgatgtc agcacgacaa
     1681 tgcccagaat aataccgcgc cggtttgccc ctctcatgtg cgtattgtaa tgcaactccc
     1741 tcaggccgac aagtaaaaag cccctgaatg taccatccag gggctcggta aatcaggaac
     1801 tctcgtcagg tcaggagtcg cccttttcct caaaccattg acgtaatcga ctcacggact
     1861 tgatgacatg agtcttctgt tccatttcga gccctagaac cggcatctgg tcacgagctc
     1921 gcagccattg aatcagaggg gcaaatttga cggaaccatc gccaatggcg atgtgcaggt
     1981 cttcggcacc gaggttgtca tgcagatgca gttcgaagat ccgtgggccg agaacgtcga
     2041 gccatttcgc catgggtttt tcggagaaaa tattgaagtg cccgacatcg aaacagacac
     2101 cgacgatgtc agccgggtag cacccaatga gttccgccag ttcgttccag gaatcgtcgt
     2161 agatgttctc gaacgcgatc ttcaggccca gcgagcgagc tttttcaacc acctgatcaa
     2221 gcaccggtct ggctcgcgtc agccagttgc cgaaatcttt cttgagcacc cacgggccat
     2281 acccgggatg cagaatgaca tgtcgagcgc cgaacttctt gcccagatcg agcgcccaat
     2341 gataacgatc acctgccacc tggcgaactt tggcgtcatg cgcccccgga ttcaaatcgt
     2401 aaaacggcgc gtgaaacgtc ggctgaaacg cacgccgatt catctcttcc ctgatctggt
     2461 caagtctttc aggggtaact gagtctgcat catcgccaga aaagtacaac tcagggtgca
     2521 gatgttgcag gccaaggaac tcgaagtcct cgggtctcat gtaacggaac gcgatattga
     2581 cgtgaatctc cgggttctgc tggctcaccg tggggctccc tggccactcg aagggttgtc
     2641 ctccgggggg atggtggctt catcgatcag catgactgga atatcgttgc gaatgggata
     2701 tcgacgtcgg cacgagacac agttcagggt gtccttggct tcatcgtgca cgatcgtacc
     2761 gtgacaggca gggcaggcca gaatgtcgag caaatctttc ttgatgggca tgaatagagc
     2821 tccttgccaa ggtcttccga gcggggtcag tataggcgga atcaggggaa attgcaaccc
     2881 accgacgctt ctgttattgt gcctcccatg ccgggaatgc tcactctgag actgaaggga
     2941 atgggggata tcgttcacct gatcccctcc cttgagcgat tacggcatct tttcccagat
     3001 gatccactgg gtctggtatg ccagaccccc ttcggagaca tcgttccaca ccgcctgg
//
LOCUS       DAORHD010000526         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36857_length_3056_cov_3.276241, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000526 DAORHD010000000
VERSION     DAORHD010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36857_length_3056_cov_3.276241"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKO06_11545"
     CDS             complement(<1..267)
                     /locus_tag="PKO06_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70324.1"
                     /translation="MPFSVRLRSGPKLSKVLQNQFFQRFWSVAKPLNLLPLFSISANL
                     LILLVGRPANSLPVAGQEASMAEKNINIRRRLSPGSLVLAWFTVF"
     gene            complement(498..2372)
                     /locus_tag="PKO06_11550"
     CDS             complement(498..2372)
                     /locus_tag="PKO06_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV70325.1"
                     /translation="MNNQHAETQRRHPRWYYVRWIARQYQGHGLTVLLMLVLTLLATT
                     AAVLLPQLLRAIIDQLTGSLEGFRTGRLTTETALAERNRSLLLLLALGFGPVVGALYP
                     WLRMCMNLWFERLFRERYLRTVLGREAEFFLRFQTGDLVTRLTDNLRCMPSGLPWLCC
                     SGIFRAVTAAGIILCCLIGMFSLHGHLALAALVPLPCMLWLFLRLQTTMEKRCEAVQE
                     RTSETTTFLETAFTGIRILKSFTAERPRLTAFRELLDRRRTHEMAQARTEGLFQVYFE
                     LLTYLGEVLVLVWGGLLVVRGELSIGSYYAFFSYLGMILPTVMDIPMLLVTLSQSFVI
                     IDRLEALQATTTTGCVATRSSELEVTSAVAATNVGEFQTLDLRGVEFAYPGQTVRDGF
                     RLQHVSFTMRAGEKVAIMGAIGSGKSTLLQVVAGLLEPTMGQVHVNGQRLSPDNSRPW
                     REQIGFVQQEPVLFSESVADNIDFWRGYESDRLEWAAELAQIRDEIGRLPGGYTERLG
                     ARGAGLSGGQRQRLALARALAGRPRLLLMDDVTAGLDAENERRLWRRLRVAAPDLACL
                     IVTHRAATARIADRILVLDRGRLVATGTHAELARSCPLYRRLAGLQETSNFRRQPLAA
                     "
     gene            complement(2383..>3056)
                     /locus_tag="PKO06_11555"
     CDS             complement(2383..>3056)
                     /locus_tag="PKO06_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV70326.1"
                     /translation="GKSTCINLLLRFHDPTGGAILVDGKDLRTIAPDQWRKKLALVQQ
                     EIHLFPGTVMENLKAFAEDVHDGRVLAAARELGAHTFIERLPQGYQTRLAERGANLSL
                     GERQLLCYVRALVRNPQLLILDEATSAVDAVTEQQLQTAMQQLMRGRTAIMIAHRLST
                     VMSADQILVFDRGRLIESGTHRELMGQKGVYRQLASLQGLDPGIAKPMARRSRARERA
                     TVCGG"
BASE COUNT          669 a          984 c          849 g          554 t
ORIGIN      
        1 aaaaaccgtg aaccaggcaa ggaccagcga tcccggcgac aggcggcgac gaatgttgat
       61 gtttttttca gccattgacg cctcctgacc cgcgacgggc aaggagttcg caggccggcc
      121 caccaggagt atcaatagat ttgccgaaat cgaaaagagc ggcaagaggt ttaacggttt
      181 cgccacagac cagaatcttt gaaaaaattg attttgtaag actttcgaga gtttcggccc
      241 gctgcgtaac ctcaccgaga acggcaaaga ccggtaccgc cgtgacgctc cggtgcttct
      301 cgaagacatc gaacacatca gtatgacctt cgacacctct gattttgcgg ggttcgtggc
      361 tgaagcccgc tgatacccac cgagaaccgg cccttcaggc agatgcctga agggcctttt
      421 gcttcctcac atccattcag cgccgaaacc gttctcaagc agaattccgg ttcaggcaac
      481 ccgtacattg aggcgactta cgccgccagt ggctgtcgac gaaagttcga ggtctcttga
      541 agacctgcca gccgccgata cagcggacag cttcgtgcca gttccgcatg cgtaccggtc
      601 gcgaccagcc ggccacgatc gagcacgaga atacgatcgg caatacgcgc agtcgcggcc
      661 cggtgcgtca cgatcagaca ggcgaggtcc ggagccgcca cgcgcaaccg acgccacagg
      721 cgacgttcgt tttccgcatc aagaccggcg gtgacatcgt ccatcagcag cagtcgcggt
      781 cgaccggcca gggcacgagc caacgccaat cgctggcgct gcccgcccga cagaccggca
      841 ccacgcgcgc ccagtcgctc ggtatagccg cccggcaaac gtccgatctc atcacgaatc
      901 tgcgccagtt ctgcagccca ttcgagccga tcgctttcat agccgcgcca gaaatcgatg
      961 ttgtcggcaa ccgactcaga aaacaggacc ggctcctgct gcacaaaccc gatctgctcg
     1021 cgccacggac gactgttgtc tggggagagc ctttgaccgt tcacgtgaac ctgccccatc
     1081 gtcggctcga gcaagcctgc caccacctgc agcagcgtcg acttgccgga accaatcgcc
     1141 cccatgatcg caaccttctc acccgcgcgc atcgtgaatg agacgtgctg cagacgaaag
     1201 ccatctcgga cagtctgccc aggataggcg aactcgactc cccgcagatc cagggtctga
     1261 aactcgccca cattggttgc tgcaacggct gacgtgactt cgagctcgga cgatcgagtc
     1321 gcgacgcacc cggtggtcgt tgtcgcctgc agtgcctcga gacggtcgat gatcacgaac
     1381 gactgcgaca gcgtcaccag cagcatggga atatccatca ccgtcggcag gatcattccc
     1441 aggtagctga agaaggcata gtacgagccg atcgaaagct ctcctctgac gacaagcagg
     1501 cctccccaca ccagaacgag aacttctccc aggtaggtca acagttcgaa gtagacctga
     1561 aacagcccct cggtgcgggc ttgcgccatc tcgtgcgtac gccggcggtc gagcagttct
     1621 ctgaaggctg tcagacgagg tcgttcggcg gtaaacgact tgagaatgcg aattcccgtg
     1681 aacgccgttt ccaggaacgt cgtcgtctct gaggttcgtt cctgcaccgc ctcacaccgt
     1741 ttttccatcg tcgtctgcag tctgagaaag agccacagca tgcacggcaa tggtaccaga
     1801 gccgccaggg ccagatgccc atgcagcgag aacatgccga tcagacaaca gagaatgatt
     1861 ccggccgccg tgaccgcgcg aaagattccc gaacagcaga gccagggcag ccccgacggc
     1921 atacaccgca gattgtcggt caagcgcgtc accaggtcac ccgtctgaaa ccgcagaaag
     1981 aactccgctt cccgccccag gacagtgcgc agatagcgtt cgcggaacag gcgttcgaac
     2041 cacaggttca tgcacatgcg cagccacgga tacagcgcgc cgacgaccgg accgaacccc
     2101 agagccagca gcagaagcag cgagcgatta cgctcagcca gagccgtttc tgtcgtaagg
     2161 cgcccggtac gaaaaccttc gagactgccg gttaactgat cgatgatcgc gcgcagcaac
     2221 tgcggcaaca gaacggctgc cgtcgtggcc agcaaggtca gaaccagcat cagcagcacc
     2281 gtcaagccat gcccctgata ctggcgcgca atccagcgca cgtagtacca gcgcggatga
     2341 cggcgttgcg tttctgcgtg ttgattgttc atcgtcattg tcttaccctc cacaaaccgt
     2401 cgcccgttcc cgggcgcgtg aacgtcgagc cattggcttc gcgatacccg ggtcgagccc
     2461 ctggagcgat gccagttgtc gatagacacc cttctgtccc atcagttcgc ggtgcgtgcc
     2521 cgactcgatc agtcggccgc gatcgaacac cagaatctga tcggcagaca tgaccgtcga
     2581 cagtcggtgg gcgatcatga tcgcggttcg gccacgcatc aactgctgca tggcagtctg
     2641 cagctgctgt tccgtcaccg catccaccgc cgacgtcgcc tcatcgagaa tcagcaactg
     2701 cgggtttcgc accagggccc gcacgtagca caacagttgt cgttcgccca gagacagatt
     2761 ggcgccgcgt tcggccagcc gggtctgata cccctgtggc agacgttcga tgaaggtatg
     2821 cgcgcccagc tctctggccg cggcgagcac tctgccgtca tgcacgtctt ccgcgaaggc
     2881 cttcaggttc tccatcaccg ttccggggaa gagatggatc tcctgttgta ccagtgcaag
     2941 cttctttcgc cattgatctg gagctattgt tcgaagatct tttccgtcta cgagaatagc
     3001 gccaccggtc gggtcatgga accgcagcag cagattgata caggtactct tgccgc
//
LOCUS       DAORHD010000527         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36858_length_3056_cov_3.204598, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000527 DAORHD010000000
VERSION     DAORHD010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36858_length_3056_cov_3.204598"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="PKO06_11560"
     CDS             complement(<1..147)
                     /locus_tag="PKO06_11560"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70327.1"
                     /translation="MISWLSGRHRRSGLTLIEMSVVVAIIGILYLTVMPMYASTIQRA
                     KETAL"
     gene            complement(144..1184)
                     /locus_tag="PKO06_11565"
     CDS             complement(144..1184)
                     /locus_tag="PKO06_11565"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV70328.1"
                     /translation="MDLKQLINTVFQQGGSELHLKVGSQPLMRKNKNLKQLQMPAVQN
                     DDMIKIVKTMLLQDEAKKFVDQSSFEKNVIGELPCNYRLNLFRSQGQILGIVHIIRQA
                     MPSFSEISFPQVFDSFTKARQGLFIIAGPARSGVSTSLAAFVERINQTRSSHILLIED
                     PIEFTFDPRLARISQRQLKKDLQTVEQGINFAKRSDVDVLVIGDVKREVPYKTILEYV
                     NGGHFVVLSMQTLGIQNTLDKIIISQPESEREHICNMLARDLLGICSQALVFHAIEQK
                     LMPIHETLIMNNTMKGIIQKARINQLEANIMSGGEGSFLFEHNLNRLVRENKIPKESA
                     DGFINFYRGSRA"
     gene            complement(1187..2254)
                     /locus_tag="PKO06_11570"
     CDS             complement(1187..2254)
                     /locus_tag="PKO06_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV70329.1"
                     /translation="MPDLLTLLKDLRQANTSDVHITPDTPVFVRKNGFLEPLKDCRFS
                     EADIKKIILGTSTPKAREILGKSRQVNYAFLCDGVPYRFAAFFDKGRFALTIRIIPAV
                     PPKFADLGLPDTLKKALSKNSGLIIVSSPSGQGKTTTIASILDFINTHFEKNIITVEN
                     PVEIRFKDDKSSFIQRSIPLDVPNFYEGLSEAYRLDPDIVVSDSLNYRDAMDQALFLC
                     EAGCLVIGATDGGTCQQVLERIIYSRPAEERDSLRGKLATHLNVLISQKLAPRSDHPG
                     QVGIFDILVNTPQVKSLIRNENLVMLKTIQEQDSASSMQTFDRHLMNLVRKNVISAAT
                     AVALADDSFEMAARFSTKGRA"
     gene            complement(2337..>3056)
                     /locus_tag="PKO06_11575"
     CDS             complement(2337..>3056)
                     /locus_tag="PKO06_11575"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70330.1"
                     /translation="AAGEIGTFIQDPESFSEALTKALTPEVHKDMEGLAGAAPPPPSR
                     PPEESVLHKCIELFQQERYDDAIPILVKYRHWFPENQEALYHLAACHHRKGSVDEAIP
                     LLEELLKLNQAHVQGGLLLGQIYFQRKDWPSLAVAYEKFRAHLPATDRKALAQVQGAL
                     GLAYFHQKKYPKALEMLKAAMAINARDLSSAYHLALTYIAMKDHGSATRILRALQSNL
                     PPNSQVLKNVEDLLQKMEEEG"
BASE COUNT          630 a          841 c          875 g          710 t
ORIGIN      
        1 cagtgccgtt tctttcgcgc gctgaatggt cgaagcatac atgggcatga cggtcaaata
       61 cagaatgccg atgatggcca cgaccacgct catttcaatg agcgtcagcc ccgaccgacg
      121 atgcctgcct gaaagccagc tgatcacgcg cgtgaacctc gatagaagtt gatgaatcca
      181 tccgcggact ctttgggtat tttattctca cgcacgagac gattcaagtt gtgttcgaac
      241 aaaaaactgc cctcgccgcc gctcatgatg ttggcttcga gctgattgat gcgcgccttc
      301 tgaatgatgc ctttcatggt gttgttcatg atcagcgttt cgtggatcgg catcagtttc
      361 tgttcaatgg catggaacac cagcgcctgg gaacagatgc ccagcaggtc gcgcgccagc
      421 atgttgcaga tatgctcgcg ctcggactcg ggttgggaaa tgatgatctt gtcgagcgtg
      481 ttctgaatgc ccagcgtctg catgctcaac accacgaaat gcccgccgtt cacgtattcg
      541 aggatggttt tgtacgggac ctctcgcttg acatcgccga tcaccagcac atcgacatcg
      601 ctgcgtttcg cgaagttgat tccctgctcg acggtctgca gatccttttt cagctgccgc
      661 tgggagatgc gggcgagacg cggatcaaac gtgaactcga tcgggtcttc gatcagcaga
      721 atgtgcgagg aacgggtctg attgatgcgt tcaacgaagg cggccagaga ggtcgagacg
      781 cccgaacggg ccggcccggc aatgataaac aggccctgtc gcgccttggt gaacgaatcg
      841 aacacctgcg gaaagctgat ttcactgaaa ctgggcatgg cttgccggat gatatgcacg
      901 atcccgagaa tctgcccctg cgaccgaaac agattcagac gatagttgca gggcaactcg
      961 ccaatgacgt tcttctcgaa cgatgattga tcgacgaact tcttggcttc atcctgcagc
     1021 aacatggtct tgacgatctt gatcatgtcg tcgttctgca cggccggcat ctgcagctgc
     1081 ttcaggtttt tgttcttgcg catcagcggc tgtgacccaa ccttgagatg cagttccgaa
     1141 ccgccctgct gaaatacggt gttgatcagt tgcttgaggt ccatgctcac gccctcccct
     1201 tcgtgctgaa tctggcggcc atctcaaagc tgtcgtcagc cagggcgacg gccgtggcgg
     1261 cggaaatcac gttcttgcgt accagattca tcagatggcg atcgaatgtt tgcatgctcg
     1321 aagcggaatc ctgttcctga atggttttca gcatgaccag attttcgttg cgaatcagcg
     1381 atttcacctg cggcgtgttc accagaatgt cgaagatgcc cacctgaccc gggtggtcag
     1441 agcgaggcgc gagcttctgg ctgatgagca cgttgagatg ggtcgccagc ttgccgcgca
     1501 gagagtcgcg ttcttcagcc ggacgggaat agatgatgcg ttcgagaacc tgctgacagg
     1561 tgccgccgtc ggtggcgccg atcacgagac acccagcttc gcagagaaac agcgcctggt
     1621 ccatggcgtc gcgatagttg agcgagtcgc tgaccacgat atcagggtcg agccggtagg
     1681 cttcggagag cccctcgtaa aagttcggca cgtcgagtgg aatactgcgc tggatgaacg
     1741 aggacttgtc gtccttgaac ctgatctcga ccgggttttc gacggtgatg atgttcttct
     1801 cgaaatgcgt gttgatgaaa tcgagaatac tcgcgatcgt cgtggtcttg ccctgccccg
     1861 acgggcttga gacaatgatc agccccgagt tcttcgacag agccttcttc agcgtgtcag
     1921 gcaagccgag gtcggcgaac ttcggcggca ccgctggaat gatgcgaatc gtcagcgcga
     1981 aacgcccttt gtcgaagaac gcggcaaagc ggtacggcac cccgtcacac agaaaggcgt
     2041 agttcacctg gcggctcttt cccagaatct cgcgtgcctt gggcgtgctg gtgccaagaa
     2101 tgattttctt gatatcggct tcgctgaacc ggcaatcctt cagcggttcg agaaacccgt
     2161 tcttgcgcac gaaaaccgga gtgtcgggcg tgatgtgcac gtccgacgtg ttcgcttggc
     2221 gaaggtcctt gagcagtgtc agcagatcag gcatgtacgt tcccttccac tattcctgat
     2281 aaacgaccat acgaggcgtc tctctaccta tcggaaaacc cgagcccaaa ccgaacctat
     2341 ccttcctctt ccattttctg cagaagatct tcgacgtttt tcagaacctg gctgttcggc
     2401 ggcagattgc tctgcagggc gcgcagaatg cgggtggccg agccgtggtc tttcatcgcg
     2461 atgtaggtga gcgccagatg ataggcggaa ctcagatcgc gggcattgat agccatcgcg
     2521 gccttgagca tctcgagcgc tttcggatac ttcttctgat ggaaatacgc cagtcccagc
     2581 gctccctgca cctgagccag agcttttctg tcggttgcgg gaagatgcgc ccgaaacttc
     2641 tcgtaggcca ccgcgagaga aggccagtct tttctctgaa agtagatctg cccgagcagc
     2701 aggccgccct gaacgtgcgc ttgattcagc ttcaacagct cttccaataa tggaatggct
     2761 tcatcgacgc ttcccttgcg gtgatggcat gccgccagat ggtacaacgc ctcctgattt
     2821 tcggggaacc agtggcgata cttgaccaga attggaatgg cgtcgtcgta tcgttcctgc
     2881 tggaacagtt cgatacactt gtgcaacacc gactcttcgg gcgggcggga gggcggtggt
     2941 ggagccgcac ccgcaaggcc ttccatgtct ttgtgaactt cgggcgtaag cgctttggtc
     3001 agagcttccg agaatgactc cgggtcctga atgaacgtgc cgatttcacc ggctgc
//
LOCUS       DAORHD010000528         3056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36861_length_3056_cov_2.870377, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000528 DAORHD010000000
VERSION     DAORHD010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36861_length_3056_cov_2.870377"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="PKO06_11580"
     CDS             complement(<1..761)
                     /locus_tag="PKO06_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70331.1"
                     /translation="MNERTRYFLAGTLAAGLTVSLLSDVNTLQAISNRVAYSHLTSEL
                     RMGIDIGLLDPADARGGNLDAPLSRREFGLLLKKTLQMLGSPAGSEVSDLSLYGVFDM
                     KNTKGSISRKEVLESLCRSVIHLADYGFVTIKEAKAADFSDYSPMPKYATAFGYLRAE
                     NVVRGYGAAFGVKRAVSRREAVTFLYRLYEKVAAARVRPSQDKKIAFIDISEDHPVME
                     QIRGLHEAGAFDAIDIQPSFDGDRTMKLKDLCAMV"
     gene            complement(838..1878)
                     /locus_tag="PKO06_11585"
     CDS             complement(838..1878)
                     /locus_tag="PKO06_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70332.1"
                     /translation="MKAAGAVRTWVWASLLLVLAAAALWSAKAREAARAVEPAEMAAI
                     VGARAAVPLVLRQVAAGFCLEQAETCLHRGAELPGAAQGRTPAVLFWLSQALAWHPEH
                     LEVWETLGNEYFNHFGDFRLGRHVLQEGIRANPRHPRRHALWAAVGIACWYQAVAAEA
                     SDTMRFSGIKYLRRAEEDALGHGLSHGSEPVMNVRAYAVFQARLHAELGDFDASWRDW
                     QRAGEETKADSELGRLLRDWKQGADPVVLRREVLDLRRASALAPATSLLAPAPLVTDA
                     LTGCNAGCEHAGHQHQTGGGQHGVEPDAAARPATEAAMVGRRVSVRLGGTFLLLSAAL
                     LMRRRRRVQTRD"
     gene            complement(1875..2633)
                     /locus_tag="PKO06_11590"
     CDS             complement(1875..2633)
                     /locus_tag="PKO06_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70333.1"
                     /translation="MSVSAKSPFRAHVAQYGWLIVSLTALSFFLCLFGLSASSGMAPE
                     VIGRDLAWFCVYWSAVVLLLVASGTPPSNLSEPSAGGVPTSWRKAFTGLLAFWLAGTV
                     SVALAVTLGLYVATGTIWSGFLIMVTGTLVGLLPLSALAILLPSRGRLLTQRSLLLIC
                     LTLGHNLDALRSHLDTLGGGWWGQVFEIVAVLIPDQGCPDWRGWLVFGQGPELFVLGS
                     AALSSLALTGFFLLLAEPRHLDRPSDACQPSSNR"
     gene            2718..>3056
                     /locus_tag="PKO06_11595"
     CDS             2718..>3056
                     /locus_tag="PKO06_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV70334.1"
                     /translation="MRLHPRFAAGSARLLALMLGLVLLLCHSGDLAAEPDYKVFTLDN
                     GLRVFIKELPSAPVVALNIWIQVGSRNEKAGQEGYAHLMEHMLFKGTPTHPTGALDRE
                     IKKLGASNNAF"
BASE COUNT          681 a         1006 c          839 g          530 t
ORIGIN      
        1 cacaccatgg cgcacaggtc tttcagcttc atggtgcgat caccgtcgaa actgggctgg
       61 atatcgatgg catcgaatgc gccggcttca tgcaaaccgc gaatctgttc catgaccgga
      121 tgatcttccg aaatgtcgat gaacgcgatt ttcttgtcct gactcggacg cacgcgagca
      181 gccgcgacct tctcataaag tcgatacaga aacgtcaccg cctcacgccg actgaccgcg
      241 cgctttacgc cgaacgccgc tccgtacccg cgcacaacgt tttccgctcg caggtatcca
      301 aacgcagtcg catacttcgg catggggcta tagtcactga aatccgcggc tttggcttcc
      361 ttgatggtca caaaaccgta gtcggccaga tgaatgacgg aacggcacaa cgactccagc
      421 acttccttgc gggaaatgct gcccttggtg ttcttcatgt caaagacgcc gtagagactc
      481 agatctgaga cttcagagcc ggccggcgat cccagcatct gcaaggtctt cttcagcagc
      541 aggccaaact cgcgccgact caacggggcg tcgaggttgc ctccacgggc atctgccggg
      601 tcaagcaaac cgatatcgat gcccatgcgg agttctgaag tgagatggct gtacgccact
      661 cgattcgaaa tcgcctgcag cgtattcacg tctgaaagga gtgagacggt aagtcccgcc
      721 gctaaagttc cggcgaggaa atagcgggtg cgttcattca tggtatggcc tccttacctt
      781 atttcccccc atgtatcggc aagaacgggg aaatcgtgaa gggagaccgc gatcccctca
      841 gtctctcgtc tgcacccgtc ttcgtcgccg catcaacagc gcggcgctca acaacagaaa
      901 cgtgccacca agccgcacgg aaactcgcct tcccaccatc gcggcctcag ttgcgggccg
      961 cgccgcggcg tctggttcga caccgtgctg accgccaccg gtctgatgct ggtgtcccgc
     1021 gtgctcacag ccggcgttac acccggtcaa cgcgtcagtc acgagtggag ctggcgcgag
     1081 cagtgaggtc gcgggagcca aagcagaagc tcgccgcaga tcgagaacct cacgccgcaa
     1141 cacgaccggg tctgccccct gcttccaatc tcgcagtagg cgtcccagtt cgctgtcggc
     1201 tttcgtctct tcacctgcgc gttgccagtc ccgccaactc gcgtcaaaat cgccgagttc
     1261 cgcatgcaga cgcgcctgaa acaccgcgta ggcacgcaca ttcatcaccg gttcagaccc
     1321 atgagacaac ccatggccaa gcgcgtcttc ttcagcacga cgcaggtatt tgatccccga
     1381 gaaccgcatc gtatcagaag cttcggcggc gacagcctga taccagcacg caattccaac
     1441 agccgcccac aacgcatgtc ggcgcggatg ccgcgggttg gcgcgaattc cttcctgcag
     1501 cacgtgtcgg cccaggcgaa aatcgccgaa atgattgaaa tactcgttgc cgagcgtctc
     1561 ccagacttcg agatgctcgg ggtgccaggc cagcgcttga gacagccaga acagaaccgc
     1621 gggcgttctt ccctgcgctg cgcccggcag ttctgccccg cgatgaagac aggtctcggc
     1681 ctgctccaga cagaatccgg cagccacctg cctgagcacc agcggaactg ccgctcgtgc
     1741 cccgacgatc gcggccattt cagcaggctc gaccgctcgt gccgcctcac gcgccttcgc
     1801 cgaccacagc gccgcggcgg cgagaaccag gagcagcgat gcccataccc acgtgcgcac
     1861 ggcaccggcg gctttcatcg attcgaactc ggctgacagg cgtcactcgg tctgtccaga
     1921 tgacggggct cggccagcag cagaaaaaat ccggtcaacg ccagagagga aagtgccgcg
     1981 gagcccagaa cgaacaactc gggtccctga ccgaacacca gccagccacg ccagtccggg
     2041 cacccctgat ccggaatcag gacggccacg atctcaaaca cctgacccca ccacccaccc
     2101 ccgagtgtgt caagatgact gcgcagagcg tccaggttgt gtccaagtgt cagacagatc
     2161 aagagaagac tcctctgggt caacaggcga ccacgcgacg gcaacagaat cgcgagagcg
     2221 gacaggggta acaagccgac aagagtacca gttaccatga tgagaaagcc actccatatc
     2281 gttcctgtag cgacatacaa cccgagtgtc acggccaatg ccacgctgac cgtgccagcc
     2341 agccagaagg ccaggagacc ggtgaaggct ttgcgccagc ttgtcggcac cccgccagcc
     2401 gaaggctctg acaggttcga aggcggagtt cccgaggcga caagcagcag aacgacagcg
     2461 ctccagtaga cgcagaacca ggccagatca cggccgataa cctcgggcgc catgcctgac
     2521 gacgcgctca acccgaacaa gcagagaaaa aagctcaggg cagtcagaga cacgatcagc
     2581 cagccatact gcgccacatg cgcccgaaag gggctcttcg ctgagacact catgcgctgc
     2641 agcatagcgc atcactctgc cgcgagcaaa ccggatgtgg tacaataggc caacattttc
     2701 tgaaggagaa attcttgatg cgcctgcacc ctcgcttcgc cgccgggagc gctcgattgc
     2761 ttgctctcat gttgggtctg gttctgctcc tctgccactc cggggatctg gccgccgagc
     2821 ctgactataa ggttttcacc ctcgacaacg gcttgcgcgt ctttatcaag gaactgccct
     2881 cggctcctgt ggtcgcgctc aacatctgga tccaggtcgg atcacgcaac gagaaggcgg
     2941 gccaggaagg ctatgcccat ctcatggagc acatgctgtt caagggcact cccacccatc
     3001 caaccggggc acttgaccgg gagattaaaa aactcggagc ctcgaacaat gccttc
//
LOCUS       DAORHD010000529         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_36899_length_3054_cov_4.175392, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000529 DAORHD010000000
VERSION     DAORHD010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36899_length_3054_cov_4.175392"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKO06_11600"
     CDS             complement(<1..337)
                     /locus_tag="PKO06_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70335.1"
                     /translation="MKVTVTTRTVLLFLCGTLPLMSASSHLGALQICIAVNLVLALLL
                     YVDWRLSPHPNQLEVGRLCPGKLSIGEPNEIVIQVTNHTFRALELTIRDEFPHPFRAV
                     RANQAAEGRQ"
     gene            complement(401..1360)
                     /locus_tag="PKO06_11605"
     CDS             complement(401..1360)
                     /locus_tag="PKO06_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009642274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV70336.1"
                     /translation="MIDLARVKEIFIKLKTEMQKVIVGQDPVLNLILTGLLAKGHILL
                     EGVPGTAKTLTVKTLAQIIGAKFQRLQMTPDLMPSDIIGTNIFDQRTASFQVQKGPIF
                     ADLVLVDEINRAPAKTQSALLECMEERQITIEGQLFPLPPLFMVLATQNPLEYEGTYP
                     LPEAQLDRFLFKGLVSYPEAKDENQILERYETGFRSAELKNVGLESVVSLEEMQACQQ
                     AIGQVKTKPALIEYIATIVRTTRSTNEVLVGGGVRASISLLLASKANALIEGRDFVTP
                     DDVKLMAPPVLRHRMILKPETEIEGVTTDEILQRILNQVKVPR"
     gene            complement(1353..2597)
                     /locus_tag="PKO06_11610"
     CDS             complement(1353..2597)
                     /locus_tag="PKO06_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNV70337.1"
                     /translation="MNLLRGYAILLLSLFIFTMWGLNLVSLSKFEKEVPDESKRRTIT
                     TEEERVETTQKQIQPRPFLAFFLRRLGYTIDFSSAPPAEPEGLAIIIDPLTGMNQERM
                     RAFYSWCQSGGHLLIITRQSHPLAQVFGADHDNRIGMRDENLQYTFSWLSDASRLSVD
                     QGGLNNRRGMSFLPLFSTRHGKPLVLSTWRGHGSATMMSGYGFCDADALSRGNNAIFL
                     ARLVRRLAPKMRVRFYDPDPLSAVQVRIKKVKVNIDVPPPMKKEMEPYLSLWSLVRAN
                     PISWVLLQLLIGLALYFLAIGRRLGQPVPLPPLEPPPQHFLESMGRLYVRFGTPAAVA
                     AQMASWHLRSLKRRYRLPSDTLVSDVCAQIVQATPDERKRLQTLLVQLQEMGGQESLD
                     PVRLVHHLRLLETMRKELKIHD"
     gene            complement(2594..>3054)
                     /locus_tag="PKO06_11615"
     CDS             complement(2594..>3054)
                     /locus_tag="PKO06_11615"
                     /inference="COORDINATES: protein motif:HMM:NF024948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HNV70338.1"
                     /translation="LYWILGTLVAIVIVIVGFLIYRAWARSRALSSHKPLVLGSSALE
                     DEIRQRRNVDLYQQALEEARKGNYEMGVRLMALAILLLLDQRGRLNFDKARTNGEYRL
                     RILDEHPALGQVMDSPLRVFDRLYYGFRPSSETDFQEFRQVFDKLERNLT"
BASE COUNT          670 a          803 c          928 g          653 t
ORIGIN      
        1 cttggcggcc ctcggcggcc tgattggcgc gtacggcgcg aaacggatgc ggaaactcgt
       61 cgcggatggt gagctcaaga gcgcggaagg tatggttggt cacctgaatg acgatctcgt
      121 tcggctcacc gatcgacagc ttcccggggc aaagtcgacc aacttcgagc tggttcgggt
      181 ggggagacaa ccgccagtcg acatagagca acagagccaa caccagattc acggcgatac
      241 agatctgcag tgcgccgaga tgggaagaag cggacatcaa tggcagggtg ccgcacagaa
      301 acagcaggac cgttcgggtg gttacagtca ccttcatgct gctgcctccg agcttatgcc
      361 atgaatgtcc gagttcggaa atatcataag ttacttcaga tcagcgcgga acctttacct
      421 gattgagaat ccgctgcagg atctcgtcgg ttgtgactcc ttcgatctcg gtctcgggtt
      481 tcaggatcat gcgatggcgc agcaccggcg gggccattag cttgacgtcg tcgggagtca
      541 cgaagtcgcg gccttcaatc agagcgttgg ctttgctggc gagcagaagc gagatcgagg
      601 cacgcacacc accacccacg agcacttcgt tggtgctgcg cgtggttcgg acaatggtcg
      661 cgatatactc gatcaatgcc ggtttggtct tgacctggcc gatggcctgc tgacaggcct
      721 gcatctcctc gaggctgacg actgattcca gaccaacatt cttgagttcc gcagaacgaa
      781 agccggtttc ataacgctcg agaatctgat tctcatcctt cgcttccgga tagctgacca
      841 gccccttgaa caggaaacgg tcgagctgcg cttcgggaag tggataggtc ccttcgtatt
      901 ccagcggatt ctgtgtggcc aaaaccatga acagcggtgg cagcggaaac aactgacctt
      961 caatggtgat ctgacgttcc tccatacatt ccagcagcgc tgactgcgtt ttcgccggag
     1021 cgcggttgat ttcgtcgacg agaacgagat cggcgaagat cgggcccttc tgcacctgaa
     1081 agctggccgt gcgttgatcg aagatattcg tgccgataat gtccgagggc atgagatcgg
     1141 gagtcatctg cagacgctgg aatttggccc cgatgatctg agccagagtt ttgaccgtca
     1201 gggtttttgc cgtgccggga acgccttcga gcagaatgtg tcctttggcc aacagccctg
     1261 tcaggatgag gttgagaacg ggatcctgac cgacgatgac tttctgcatt tcggtcttga
     1321 gtttgatgaa gatttccttg acgcgggcca gatcaatcat ggattttgag ttcctttcgc
     1381 atggtttcga ggagtcggag atggtgcacg aggcgcacgg gatcgaggct ttcctggcca
     1441 cccatttcct gcagttgaac caacagagtc tgcagccgct ttcgttcgtc gggtgtcgcc
     1501 tggacgattt gagcgcagac atcggaaaca agcgtgtcgg aaggcaggcg ataacgtcgt
     1561 ttgagactgc gcagatgcca cgaagccatc tgagcggcaa ccgccgccgg agtgccgaat
     1621 cgcacataga gccgacccat ggattcgaga aagtgttgtg gcgggggctc gaggggaggc
     1681 aagggaaccg gttgtccgag acgtcgtcct atggccagaa agtaaagggc cagcccgatc
     1741 agcagctgca gcagtaccca ggaaatcgga tttgcccgta ccagactcca cagactgaga
     1801 tagggttcca tttccttttt catgggcggt ggaacgtcga tattgacttt gaccttcttg
     1861 atgcgaactt gtactgccga aagtggatcg gggtcataga agcgaacccg catctttggt
     1921 gcgaggcgtc tgaccagcct tgccagaaag attgcgttat tgccgcgcga caacgcgtct
     1981 gcatcgcaga acccgtagcc cgacatcatc gtggccgatc cgtgcccacg ccaggtggac
     2041 agcacaagcg gtttcccatg gcgtgtcgaa aacagaggca gaaagctcat gccgcgtcga
     2101 ttgttcagcc cgccctgatc cacgctgagg cgtgaggcgt cgctcagcca gctgaaggtg
     2161 tactgcaggt tctcgtctct catgccgatg cggttgtcat gatcggcacc gaagacctgg
     2221 gctaatggat gagactggcg ggtaatgatc aagagatggc ctccggactg gcaccagctg
     2281 tagaaggcgc gcatgcgttc ctgattcatg cccgtgagcg ggtcgatgat gatcgccagc
     2341 ccctcgggtt cggccggcgg agctgacgag aaatcgatgg tatagccaag gcgacgaaga
     2401 aagaaggcga gaaatggtcg tggttgaatc tgcttctggg ttgtctcgac gcgttcctcc
     2461 tcggtggtga tcgtgcgtcg tttgctctcg tccgggacct ctttttcaaa tttcgacaga
     2521 ctgacgagat tcaaccccca catggtgaag atgaagaggc tgagcaggag gatggcatat
     2581 ccgcgcagaa gattcatgtc agatttcgct ccagcttatc gaacacctgg cgaaactcct
     2641 gaaagtctgt ttctgaagaa ggtcgaaatc cgtaatacaa ccggtcgaac acccgcagcg
     2701 gggaatccat gacctgaccg agcgcgggat gttcgtcgag aatgcgcagg cgatactcgc
     2761 cgttggttcg cgctttgtcg aagttgagac gaccgcgttg atcgagcaga agtagaatgg
     2821 cgagggccat caggcgtacg cccatttcat agttcccttt gcgggcttcc tcaagggctt
     2881 gctggtacaa atcgacattg cgacgctgac gaatctcgtc ctcgagcgcg gaacttccga
     2941 ggaccagagg tttgtgtgag gacaaggccc gactgcgtgc ccaggcgcga taaatgagga
     3001 agccgacgat cacgatcacg atggcgacca gtgtcccgag aatccagtag agga
//
LOCUS       DAORHD010000530         3043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37126_length_3043_cov_3.644913, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000530 DAORHD010000000
VERSION     DAORHD010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37126_length_3043_cov_3.644913"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKO06_11620"
     CDS             <1..1009
                     /locus_tag="PKO06_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70339.1"
                     /translation="KEVEVPMKFHQSLMTRVILIFLLVFLSSGDVWAQSARARGMGGA
                     FLGVSNDESATYYNPAGLSQIQGREASLQLKVNERDYFQWGSAAFTGHIYEDEPKDQF
                     SITDYLEHNVLEEPLPRRPKYSYGVAYTQDTRKPGFGDMVGGEFKGVTREVDELQLAF
                     GTRFPIARRMLAREQLFGGVKFNFRNTDRWIRSLGTHAKRNTQSMGIGLMYHYNDRIT
                     GGLTVDNLVQHISGDDEDIRDGVSLNLGGALRLTKGTLISGDVYNVTTANRAQSQQYR
                     LGVEKKFIENDLTMRMGTWNGTLTLGFGMHLLPNLRFDYAFYNGDIVKEHYVAGHLTF
                     D"
     gene            1050..2024
                     /locus_tag="PKO06_11625"
     CDS             1050..2024
                     /locus_tag="PKO06_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70340.1"
                     /translation="MHRSFSFLPSFSLFLLALFLTAVTPAGAQISSATIDFQRQVVTA
                     WGLAGAPWEKTPSLEEERARAWMDAMHHAYEAILSLPLMEQASVRGSVLQNPALRERL
                     GRLLLGLPKTFYEPDATGLHRCRIEVPFAGRDSLRTALYLAALRPTQTEPKALIGSDS
                     AAGKATSDGIREATGTAPLRLILDLRRTTFMPSLFPRFFAEDGRLLFQESMIPATERF
                     SRPMVRFALAIEEATVGVPADRVRYVEAQVTTLGRHDVQIRKADEDLFRRFCRGLISE
                     PLREGEILIVYGNRLLAAGLLPKADRKEKTDGAASAKPKEPAKKGRNK"
     gene            2024..2977
                     /locus_tag="PKO06_11630"
     CDS             2024..2977
                     /locus_tag="PKO06_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70341.1"
                     /translation="MKNQMLNWRLLIISCVCALWYVSGGIALAEDAPPFTAQRQIKRL
                     ETALYGKNQTGNWNERLNRIELDLLGRETGLKVADRSDMLVRFLFKGTETAPSMEMKL
                     GFLEWKLFHETRQGTVADRIAAIAKQAKIKSSSEPLVFRLEQLVQAIVDNGYLTLHKV
                     KLPAGSSVRVRLSRDVSSNQAREGDLVPVSVVRDTFIDDNVLVVAKGGIALGEVEKVR
                     RSGRFGRSGNLAFAIRELHGLDGSSIPVVLKDSAFGPDQRKPIGMAVGASALGYLALG
                     PVGLAGGVFVKGKEMVLPQGLELVMSTTEDVWVVGIVVNRR"
BASE COUNT          635 a          821 c          916 g          671 t
ORIGIN      
        1 taaggaggta gaagtgccca tgaaattcca ccagagcctt atgacgagag tcattctcat
       61 ctttcttctc gtgttcctca gttcgggcga tgtgtgggcg caatcagcac gcgcgcgggg
      121 catgggcggc gcatttttag gcgtttcgaa cgatgaatca gccacctact acaacccggc
      181 gggtctgtca cagattcagg gccgagaggc ctcactgcag ctgaaggtga acgaacgcga
      241 ctactttcag tggggttcgg ccgccttcac cggtcacatt tatgaagacg aacccaaaga
      301 ccagttctcg attaccgact acctcgaaca caatgtgctt gaagaaccgc tcccgcgtcg
      361 cccgaaatac agttatggtg tggcttacac gcaggatacc cgcaaacccg gttttggtga
      421 catggtcggc ggtgagttca aaggcgtgac tcgcgaggtc gatgagctgc agctggcgtt
      481 cggcacccgg tttccgattg cccggcgcat gctggcccgc gaacagcttt tcggcggtgt
      541 gaagttcaac tttcgcaaca ccgatcgctg gatcagaagc ctcggcactc atgccaaacg
      601 caacacgcag agcatgggca tcggcttgat gtatcactac aacgatcgca tcaccggcgg
      661 cctcacggtc gacaatctgg tgcagcatat cagcggtgat gatgaagaca ttcgcgatgg
      721 tgtatctctc aaccttggtg gtgccctgcg attgaccaaa ggaacgctga tcagtggaga
      781 tgtctacaac gtcaccacgg ccaatcgtgc gcagtcacag cagtatcgtc tgggcgtcga
      841 gaagaagttt atcgagaacg acctgacgat gcgcatggga acctggaatg gcacgctgac
      901 tctggggttc ggcatgcacc tgttgcccaa tctgcgtttt gactatgcct tctacaacgg
      961 ggatattgta aaagaacact atgttgccgg acacctgacg ttcgattgac gtcttgacag
     1021 acgcacatcg atcggtgcat gaaagagtca tgcaccgatc gttttctttt ttaccgtctt
     1081 tttctctttt tctgctggcc ctgtttctga ctgccgtcac tccagccggg gcacagatca
     1141 gttccgcgac catcgatttt cagcgacagg tcgtgaccgc gtgggggctg gccggggcac
     1201 cgtgggagaa aaccccctcg ctcgaagagg aacgggcacg cgcctggatg gacgcgatgc
     1261 accacgccta cgaagcgatt ctctcgcttc cgctcatgga acaggcttcg gtgcgtggat
     1321 cggtgctgca gaacccggct ctgcgcgagc ggctcggaag actgctgctg ggtctgccga
     1381 aaacgtttta cgaacccgat gccaccggtc ttcatcgctg ccgcatcgaa gtgccctttg
     1441 cagggcgtga cagtctgaga accgctctgt atctggccgc gttgcggccg acgcagaccg
     1501 aaccgaaggc gctgatcggc tctgactctg ctgccggaaa agcgacaagt gatggaatac
     1561 gagaagccac gggaacggct ccgttacgtt tgattctgga tctgcgtcgc accacgttca
     1621 tgccgagtct gtttccgcgg ttctttgcgg aagacggccg gctgctgttt caggagtcga
     1681 tgattcccgc gactgaacga ttcagtcgac cgatggtgcg atttgctttg gccatcgaag
     1741 aggcgaccgt aggggttccg gctgaccgcg tgcgatacgt agaagcgcag gtgaccaccc
     1801 tgggtcgaca cgatgtgcag attcgcaagg cggatgaaga cctgtttcgt cgtttttgtc
     1861 gcggcttgat ctctgagccg ttgcgcgaag gggaaatact gatcgtttac ggcaaccgcc
     1921 tgctggccgc gggtctgctg ccgaaagccg atcgaaaaga aaaaaccgat ggggccgctt
     1981 cggcaaagcc gaaagagccg gccaagaaag gcaggaacaa gtagtgaaga atcagatgct
     2041 gaattggcgg cttttgatca tcagttgcgt gtgcgccctc tggtacgttt ccggtggtat
     2101 tgctctggcc gaagacgctc cgcccttcac ggcgcagcgg cagatcaaac gactcgaaac
     2161 ggcgctctat ggcaagaacc agaccggaaa ctggaacgag cgactgaatc gcatcgaact
     2221 cgatcttctc ggtcgtgaaa ccggcctcaa ggttgccgat cgcagcgaca tgctggtgcg
     2281 gttcctcttc aaaggcacag aaacagcgcc ttccatggaa atgaaactgg gctttctcga
     2341 gtggaagctg tttcacgaaa cccgccaggg aaccgtggct gatcgtatcg ctgccattgc
     2401 caaacaggcc aagatcaaat cgagttccga gcctctggtg tttcgtctcg aacaactcgt
     2461 tcaggccatt gtcgataacg gatacctcac gttgcacaag gtgaagctcc cggccggcag
     2521 ttcggttcgc gtgcgtctct cacgcgacgt ttccagcaat caggcgcgag aaggtgatct
     2581 ggtgccggtc agcgtggtac gcgacacgtt cattgacgac aatgtgctgg tggtggcaaa
     2641 aggcggcatc gcgctgggtg aagtggagaa agtgcgacgg tcgggacggt tcgggcgttc
     2701 gggaaacctg gcttttgcca ttcgtgaact gcatggcctc gatggttcat cgattcccgt
     2761 ggtgctgaaa gactcagcgt ttggaccgga tcaacgcaaa ccgatcggca tggccgtggg
     2821 ggcttccgcc cttggttatc tggcgctggg gccggtaggt ctggccggtg gtgtctttgt
     2881 caaaggcaaa gagatggtac tgccgcaggg gctcgaactg gtcatgtcca cgactgaaga
     2941 tgtctgggtg gtcgggatcg tcgtcaatcg gcgctgacct tttcatgtat ccgatactgt
     3001 tcgagtgggg ccattgcgga ttggctccta tggtgtgctg ttg
//
LOCUS       DAORHD010000531         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37144_length_3042_cov_3.983596, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000531 DAORHD010000000
VERSION     DAORHD010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37144_length_3042_cov_3.983596"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3042)
                     /locus_tag="PKO06_11635"
     CDS             complement(<1..>3042)
                     /locus_tag="PKO06_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70342.1"
                     /translation="HNRTESLLLLGMAVRAYSFARSMIKVFDRIPSYATDGTFIPILT
                     STFTPPPPPDPIVPEPVSEATASVQLPPLVIEPHEPPVIVDPKPPQRHLPKSLTLVAE
                     ADAARVRAFYTLNQGTHFEYAPDDREFKPVTARFLPGNHVVILDAGHRRVIVVDTMGS
                     IIEEADLFADADGIALPLKQPSSLAVVDGPSILVADTGNHRVLELDQQLQCLWTFGGD
                     TDEASSPFHLPLAAERLMNGHTLVIDGEKKDVVELTSSGAVVKRFGLDAGVADPICLF
                     RRAGETRFWIGETTGRISCFEESGQIVRTIARVKTQKRGNDPFGGVGEEALSGLQALH
                     ADPDGNLLAVRRAAGIVTFLHRGETIWDSIKDLQSPSSVDMGLYTPPPDVPEEYLQLP
                     PEKMVLVEPPDEEEDTEEFDPWYEERRLQEEENARLEEEAQRRAAARQVDVKVTMALA
                     ERRPRPVLREPSRTVDISGTMIFYPFAATIGDFAIPEPHYGAYQAGKGPRSHGIYVDR
                     PHIRCEDLFFRAYGAMVRNKIFETYDHPPAYKIDQRHSSTPFGLRDAWYHGWDTFFSY
                     AVRNSPTMPNLKTSDRKELSPLQLLETYAGQVTLEDETGEYKWLDELKSKAGRGLNCE
                     LAWSLIMWKLFESYGDLFLTNLRSYALNAVTPKYMPVSVMAELFADRLSDFLALGLVP
                     VIRLETPDSSIPTLTNAAPRISWHANGLLGDDADKQLKFTLLYADNIDFLNAREIPIP
                     EGKLLEFDFGAVLSEADQAAKLNNRLGNGVWYFRVRCDSDAFPNVGAKYGASSGIGAF
                     RIARPTTPVDAAGGVVNFEGGQGVTGNVTIPTGATGKPLQVSAQPISVATGTNSDDVV
                     GNAAIDFGPEGTKFNQVAEMNFRFDEKLLGGISPNTLVVKRWDAQKNQWIPIRTRVDP
                     DKQSVQAKSGSFSVYALMIDKTPPQITLLTDSPDPLGRNATDASLITMQASENGLATL
                     AILDSTGKTVRTLLNQFEFSNNACRTLWDLTDDTGRRVPDGT"
BASE COUNT          615 a          841 c          937 g          649 t
ORIGIN      
        1 cgttccgtca gggacgcggc gacccgtgtc atcggtgaga tcccacagag tgcggcaggc
       61 attgttcgaa aactcgaact ggttgagcag ggtgcggacg gtcttgccgg tcgaatcgag
      121 aatggcgaga gtcgccagac cgttctcgct ggcctgcatg gtgatcagag acgcgtcagt
      181 ggcgttgcga ccgagcggat cagggctgtc ggtcaacaag gtgatctgcg gcggggtctt
      241 gtcgatcatc agcgcataga ccgagaaact gccggacttg gcttgtactg attgcttgtc
      301 cgggtcgacg cgggtacgaa tcggaatcca ttgattcttc tgtgcgtccc agcgtttgac
      361 cacgagcgtg ttgggcgaga tgccaccgag cagtttttcg tcgaaacgaa agttcatttc
      421 ggcgacctga ttgaacttcg ttccctcggg gccgaagtcg atggcggcgt tgccgacgac
      481 atcgtccgag ttggtgccgg tggcaacgct gattggttgc gccgagacct gaagaggttt
      541 tcctgtcgca ccggtaggta tcgtcacgtt acccgtgacg ccctggccac cctcgaagtt
      601 gacgacacca ccagccgcgt cgaccggagt cgtcggtcgc gcaatgcgaa aagcgccgat
      661 tcccgaactg gcaccatatt tggcccccac gttcggaaac gcgtcagagt cgcagcgcac
      721 gcggaaatac cagactccgt ttccgaggcg gttattcaac ttggcggcct gatctgcttc
      781 agacagtacc gcgccgaaat cgaactcgag cagctttcct tccggaatcg ggatctcgcg
      841 cgcattgaga aagtcgatgt tatcggcgta gagcagggtg aatttgagct gtttgtcggc
      901 atcgtcacct aaaagaccat tcgcatgcca gctgatgcgc ggggcggcat tcgtcaatgt
      961 cggaattgat gaatcggggg tttcgagtcg aatgacggga acgagaccca gggcgagaaa
     1021 gtccgatagg cgatcggcaa aaagctccgc catgacgctg acgggcatgt acttcggtgt
     1081 gacggcattg agagcatagc tgcgcaggtt ggtgaggaac agatcaccat agctttcgaa
     1141 caacttccac atgatcagcg accaggccag ctcgcagttc agcccgcggc ctgctttgct
     1201 cttcagctcg tcgagccact tgtactctcc cgtctcatct tcgagggtca cctgtcccgc
     1261 ataggtctcc agcagctgca gaggggacag ttccttgcga tctgaggtct tgagattcgg
     1321 catggttggc gaattgcgta ccgcataact gaagaacgtg tcccagccgt gataccacgc
     1381 atcgcgcaaa ccgaaaggag ttgacgagtg tcgttggtcg atcttatagg cgggtggatg
     1441 atcgtatgtc tcgaagatct tgttgcggac catcgcccca taagcccgga aaaacagatc
     1501 ctcacagcga atgtgcggtc ggtcaacgta gatgccgtgc gatcgtggtc ccttcccggc
     1561 ctgataagcg ccgtaatgcg gctctggaat ggcgaagtct ccgatggtgg cggcaaaggg
     1621 atagaagatc attgtgccgc tgatatccac cgtgcgactg ggttcacgaa gcaccggtct
     1681 tggtcgtcgt tcagcgagag ccatcgtgac cttgacgtcg acttgtctgg cagctgctcg
     1741 acgctgcgcc tcttcctcga ggcgggcgtt ctcttcttcc tgcaatcgcc gttcttcata
     1801 ccaggggtcg aactcttccg tgtcttcctc ttcgtcgggg ggttcaacca gtaccatctt
     1861 ttccggaggc aactgcaggt attcttccgg aacgtcgggc ggcggagtgt acaagcccat
     1921 gtcgacgctt gacgggctct gaagatcctt gatcgagtcc cagattgttt cgccgcggtg
     1981 taaaaacgtg actattcccg cggcacggcg aaccgccagg agatttccgt cggggtcggc
     2041 atgcagtgcc tgcagcccgg aaagagcttc ttcgccgacg cctccgaaag gatcatttcc
     2101 acgtttctgg gttttgaccc gggcgatcgt acgcacgatc tgccccgact cttcgaaaca
     2161 ggagatacgt ccggtggttt cgccgatcca gaaacgggtt tcgccggctc gtcggaacag
     2221 gcagatagga tcggcaactc cggcatcgag gccgaaccgc ttcacaaccg ctccggagga
     2281 agtgagttca accacgtctt tcttctcgcc atcaatcacc agcgtgtgac cgttcatcag
     2341 gcgttctgcg gccaggggca gatgaaaggg gctcgaagcc tcgtctgtat caccaccgaa
     2401 ggtccagaga cactgcagtt gctgatcaag ttcgagaacc cggtgattgc cggtgtccgc
     2461 gaccagtatt gatggaccat cgacaaccgc gagactggaa ggctgcttca gtggcagagc
     2521 gattccatct gcgtcggcga acaggtcggc ttcttcgatg atcgaaccca tggtatccac
     2581 gacgatcacg cgccgatgac cggcgtccag aatgacgacg tggttgcccg gcagaaagcg
     2641 ggcagtgacc ggtttgaatt cgcggtcatc cggggcatat tcgaaatgcg tgccctgatt
     2701 gagggtgtag aacgcgcgaa ccctggcggc gtcagcctca gccaccagag tcaggctctt
     2761 gggaagatgt cgctgcggcg gcttggggtc gacaataacc ggtggttcat gcggttcaat
     2821 caccaggggg ggcagctgta ccgaagcggt cgcctcgctc accggctccg gaacaatcgg
     2881 atcgggcggc ggtggcgggg taaacgtgct ggtaagaatg ggaatgaacg tgccatcggt
     2941 tgcatagctg gggattcggt caaagacctt gatcatcgat cgggcgaacg agtaggcgcg
     3001 caccgccatt ccgagcagaa gcagcgattc ggttcggtta tg
//
LOCUS       DAORHD010000532         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37149_length_3042_cov_3.306662, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000532 DAORHD010000000
VERSION     DAORHD010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37149_length_3042_cov_3.306662"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PKO06_11640"
     CDS             <1..749
                     /locus_tag="PKO06_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNV70343.1"
                     /translation="GRVRFIGLDNFLSLAEDPAFWYSLRNILAFVLVGVPLNIVTALI
                     IALVLDHPCRNLKGISRTLFFLPTVTTLAAVALVWQRLYAVEGGLLNQICLWAGLPAI
                     NWLNHPWFALPALIIMTVWKGFGLNAAVFLAALQNVPEELYEQVEIDGGSWWHKIRHV
                     TLPFLQGSMVFVFITTTVGTFHVFTEPYMMTGGGPLYATITPVLHLYQNGFQFFRLGY
                     ASAITCTLSIGVIAFNLLQQALRRRWGHTP"
     gene            746..1549
                     /locus_tag="PKO06_11645"
     CDS             746..1549
                     /locus_tag="PKO06_11645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV70344.1"
                     /translation="MSAPGRAAAPLQTAGWLFFLLISLFVTLLPFFWMIYQSCDAAVA
                     AVSANAYRRLFAQASIPTHFVNSVIYAGGLTALSLIFNTLAGFAFACLQFPRRERLFT
                     LLLMTMMIPSQITLVPMFLFLKFFGMLNSFSGLIIPGSAHVFGIFMIRQFLRDLPEEV
                     FEAARIDGCSEWHLFRHIVLPLSRPILATLAVISFIGAWNEFLWPLIVMQKQNMYPLP
                     VALTMLNSQFYDDMGLMMAGAVVTMVPALLIFAFAQRHYIKGLTRGAGK"
     gene            1759..>3042
                     /locus_tag="PKO06_11650"
     CDS             1759..>3042
                     /locus_tag="PKO06_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV70345.1"
                     /translation="MHTRYGMGNPMGKLSAFIQAPQPLDAHGRLVFRIGRARAGDWVR
                     LAIALAFVVLAATGVFAEPLRQFTILHTNDLHSHLLPFDQVDFGKNVGGAARLAELVK
                     RITASTSNWVLLDGGDLFQGTPFYSSFFGEVEAQVAALIGYTATVLGNHDLDDGLSNL
                     RRQYASCSVPLLCANVLDKSTGKPAFTPWLVADRSEVKVGIIGAIGSAAWDVISHKRQ
                     RELTFLEHEPVVASIARHLRPQVDLVILLSHAGYEADLAFAARATDIDVIVGGHTNSI
                     TSHPVLIPHLSVPGKPASTNSLGGTLVVQAFKWGVFLGRLDLTLRADSSIATYSGQLL
                     KVDDAIATPEGSPVARLVDSYHERIKALTSQVIGHSASDLLYPDEEKHLQVFPLGVWV
                     CDAMREFVGADFGIINSGTIRDRLASGPVTMGSLLS"
BASE COUNT          558 a          934 c          815 g          735 t
ORIGIN      
        1 cgggacgagt cagattcatt ggcctcgaca actttctgag cctcgccgaa gaccccgcct
       61 tctggtacag cctgcggaac attcttgcct ttgtcctggt cggcgttccg ttaaacatcg
      121 taacggcgct gatcatcgct cttgtgcttg atcacccctg ccgcaatctc aagggaattt
      181 cacgcacgct gttctttctg ccaacggtca caacactggc ggcagtcgcc ctggtctggc
      241 aacgtctcta cgccgtcgaa gggggacttc tgaaccagat ctgcctctgg gcaggtctgc
      301 cggccatcaa ctggctgaac catccctggt tcgctctgcc ggccctgatc atcatgacgg
      361 tctggaaagg cttcggcctc aacgcggccg tgtttctcgc ggcactccag aacgtgccgg
      421 aagagttgta cgagcaggtg gagatcgatg gcggctcctg gtggcacaag atccgtcatg
      481 tcactctgcc attcctgcag ggcagcatgg tgttcgtctt catcaccacc accgtgggga
      541 cctttcacgt tttcaccgaa ccgtacatga tgactggcgg cggccccctc tacgccacta
      601 ttacacccgt tcttcacttg tatcaaaacg gctttcagtt ttttcgactt ggttatgctt
      661 cagccattac ctgcaccttg agcatcggcg tgattgcttt caacctgttg cagcaggccc
      721 tgcgacgacg ttgggggcac accccatgag cgctcccgga cgcgctgcgg ccccgctgca
      781 aacggccggc tggctcttct ttttgttgat cagccttttt gtgactctgc tgccattttt
      841 ctggatgatc taccagtcct gcgatgcggc ggtagcggcc gtctcagcga atgcgtaccg
      901 gcgtttgttt gcccaggcat cgatcccgac ccactttgtc aacagtgtca tctacgcggg
      961 tggtttgacg gctctgtcat tgatcttcaa caccctggcc ggtttcgctt tcgcctgcct
     1021 gcagtttccc cggcgggagc gcctctttac tctgttgttg atgaccatga tgattccgag
     1081 ccagatcacg ttggtgccaa tgtttctgtt tctgaaattc ttcggcatgc tcaacagctt
     1141 ttcggggctg atcattcccg gctcagcgca tgtcttcggg atcttcatga tccgccagtt
     1201 tctccgcgac ctgccggagg aggtctttga agccgcgcgt atcgacggct gcagcgaatg
     1261 gcacctgttt cgtcacatcg tgctgccgct gagtcggccg attctggcaa ctctcgcggt
     1321 catcagtttc atcggagcct ggaacgagtt tctctggccc ctgatcgtca tgcagaagca
     1381 gaatatgtac ccattgccgg ttgctctgac catgttgaac agccagtttt atgacgacat
     1441 gggtctcatg atggccggcg cggtcgtcac catggtgccc gcgctgttga tcttcgcctt
     1501 cgcccaacgc cactacatca aaggactcac ccgcggcgcc gggaaatgac acgaatccat
     1561 ttctgaattg gtcaccccct caggaagccg gatactggtt cttcaaagcc ggctactcga
     1621 acatcacctt aatttcggcc ggaagtccaa cggtcgacct gagaacgctg acctcggttt
     1681 tggtctcttc gccatcgggt ttctggttgc gggggcggcg gagggcaggt aaacttcgtc
     1741 aaggagcgac gaccacgcat gcacacacgc tacggaatgg gcaacccgat ggggaagctc
     1801 agcgccttta ttcaagcccc acagcccctc gacgctcacg gtcggctcgt tttccggatc
     1861 ggacgagcgc gagcaggaga ctgggtcaga ctggcgatcg ctctcgcatt cgttgttctg
     1921 gcggcaaccg gggtgttcgc cgaaccgctc cggcaattca ccattctaca caccaatgat
     1981 cttcactcgc atctgttgcc gttcgaccag gtcgacttcg ggaagaatgt cggcggagcc
     2041 gcgcggctcg ccgaactcgt taagcggatc accgcttcga cttccaactg ggtacttctc
     2101 gatggcggcg acctgtttca aggaacaccg ttctactctt cgtttttcgg tgaagttgaa
     2161 gcacaagttg ccgccctgat cggatatacc gccaccgttt tgggaaatca cgatctcgac
     2221 gacggtctga gcaacctgcg ccggcagtat gcctcctgtt cggtgccatt gttatgcgcc
     2281 aatgtcctgg ataagagtac tggtaagccg gcattcactc catggttggt agcggatcgc
     2341 agcgaagtga aagtaggaat catcggcgcg atcggttctg ccgcctggga tgtcatctca
     2401 cacaaacgac agcgcgaact gacgtttctc gaacacgaac ctgtcgtcgc cagtatcgcc
     2461 cgtcatctgc gacctcaggt cgatctggtc attcttctgt ctcacgccgg ctacgaagcc
     2521 gatcttgcgt ttgcggcccg cgccacggac attgacgtca ttgttggtgg tcacaccaat
     2581 tcgatcacct cgcatcccgt gctgatccct catctttcag ttccaggaaa acctgcttcc
     2641 acaaacagcc tgggcggaac tctggtagta caagcgttca aatggggagt ctttctcggc
     2701 cggctggatc tcacactgcg cgccgacagt tccatcgcca cctattcggg gcagctgctc
     2761 aaggtcgacg acgcgatcgc cacccccgag ggcagcccgg ttgcccgcct ggttgactcc
     2821 taccatgagc ggatcaaagc tctgaccagt caggtaatcg gacattccgc atccgacctg
     2881 ctctatccgg acgaagaaaa acacctgcag gtctttccac ttggcgtgtg ggtgtgcgat
     2941 gccatgcgcg agttcgtggg tgccgacttc ggcatcatca attcagggac catcagagat
     3001 cgtttggcct caggcccggt aaccatgggc tctctgctca gc
//
LOCUS       DAORHD010000533         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37151_length_3042_cov_3.014730, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000533 DAORHD010000000
VERSION     DAORHD010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37151_length_3042_cov_3.014730"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..646
                     /locus_tag="PKO06_11655"
     CDS             458..646
                     /locus_tag="PKO06_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70346.1"
                     /translation="MTSDCPNHADRRGKPDDVALAELQRCAGAHFDPRMVKAFLMAYQ
                     KEKIISQSRESASEATGS"
     gene            643..2253
                     /locus_tag="PKO06_11660"
     CDS             643..2253
                     /locus_tag="PKO06_11660"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV70347.1"
                     /translation="MTIITQNPNGQIRPEFQPLGAQEIQRSFSPYLSDESRMQGQAEA
                     ITFPQSEAEIAASLQAAAQAHEKVTVSGGRTGVVGGAVPPGGWILSVERLAQIERFRF
                     DDASGEYRLHTGPGLTLDKLRRALRSAVFPFSAPPDVDTQRVIEQYRREASQWMFAPD
                     PTEPSAQLGGMAASNASGAKTYRYGPMRAHVRGLRVVLAGGRILHLERGQHLRQLGQS
                     FTISDPGGVEWSVPVPGYQIPHTKHAAGFYAAPQFDLIDLFIGSEGTLGIISELELAL
                     VPVKGCEAQLVAFFPDAHAACHFVCRVRQNAEAERITVEAIEYMCDRSLTLLRRGAGA
                     HFRQIPDDGRTGVFLDLRFAGDPTAAWHRLAQWLRECGSSEDSCLSAMGAVHLAQVKE
                     IRHALPESVNHRISEIRAVHPQLTKLGTDMAVPDEHLNEVMELYAARLTEAGLQHVIF
                     GHIGNNHLHVNILPETPAQYERGKALYKEFAASIVKMNGSVSAEHGIGKLKRYLLEYQ
                     FSTAALAEMKAIKAALDPDFLLGPETLF"
     gene            complement(2336..2983)
                     /locus_tag="PKO06_11665"
     CDS             complement(2336..2983)
                     /locus_tag="PKO06_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70348.1"
                     /translation="MKRLVLLAGAIYGFAAMFAFSVNADSTLTIPIPDKSMSSKEVTL
                     SLRIPPNTQPITQQARPKMVSRSALYSWDANPLPKVANTDELRDILLKFVDKGWLKGI
                     SSITYLKTKQLDKYISVSMLQKTIDAVLEIAQSPNMVRTIRKSNLLASDIEDLRRMMA
                     RYTSELKMFGTNVAKTDKDLLMLQEQYKTAKSGIMKVIKVEGMGDGSTVIHLNVE"
BASE COUNT          626 a          913 c          880 g          623 t
ORIGIN      
        1 tgttcattcg gttgcatcgg gcgctttcac cttcaactgg ctgcgtcagc acagggagca
       61 acctcccaat cttattctat tcgacatgct catgcccgaa atggaggaat tcgaggctct
      121 caagaccatg cgcgccgacc ctcactggta gtctctgccc gagaatgttc tgtcagtact
      181 ggtcgccggt ccgcgcattc aggagctcgg cgtagccgcg tatctgacga agccacagac
      241 atgggtaacc ctggttcaga cagtccgaga tatcctgacc cgggcaggag aaaacccacc
      301 tggaaaaccg caagattctc gtcgctgatg acgagcccca tcttctgtga gtggtcaaga
      361 aaaagctcgc caacgcctgt tttcagtttg tcaccgcggt caacggcgaa gaagctcggc
      421 aggtcgcatc atcgcggtcg ccgatacgtt tgacgccatg acttcagact gccccaacca
      481 tgccgacaga cggggaaagc cggacgacgt cgcgctggcg gagctgcaac gctgcgccgg
      541 agcccacttc gacccacgca tggtcaaagc ctttctcatg gcctaccaga aagaaaaaat
      601 catctctcaa agtcgcgaga gtgcaagcga ggcgacggga tcatgaccat catcacccaa
      661 aacccgaacg gccagatacg gccggagttt cagcccctgg gagctcagga gattcaacgg
      721 tcgttttcgc cgtatctgag cgacgagtca cgcatgcagg ggcaggcgga ggcaatcacg
      781 tttccacaat cagaagcgga aatcgccgcc agtctgcagg ccgcggccca ggcgcatgag
      841 aaagtcacgg tttcgggagg gcgcaccggg gtggttggtg gcgctgtccc ccccggcggc
      901 tggatcctct cggtcgagcg actggcccag atcgaacgct ttcggttcga cgacgcgagc
      961 ggggagtatc gtctgcacac cggccctggt ttgacgctgg acaaactgcg acgagcgctg
     1021 cgttccgccg tgtttccctt cagcgcccct cccgacgtcg acacgcagcg cgtgatcgaa
     1081 cagtatcgcc gggaagcctc gcaatggatg ttcgcgcccg acccgaccga acccagtgct
     1141 cagctcggcg ggatggccgc cagcaacgcc agcggtgcca agacctaccg ctatggtccg
     1201 atgcgcgcgc acgtgcgcgg gctgcgcgtc gtgcttgccg gggggcgaat cctgcatctc
     1261 gagaggggtc aacatcttcg ccaactcgga caatcgttca ccatttccga cccgggcggc
     1321 gtggaatggt cagtcccggt acccggctat cagattccgc ataccaagca cgcagccggc
     1381 ttctatgccg ctccacagtt cgatctgatc gacctgttca tcggctcgga aggcacgctc
     1441 ggcatcatct ccgaactgga actggctctg gttccggtga aaggatgcga agctcagctg
     1501 gtcgcctttt ttcccgacgc gcatgccgcc tgtcacttcg tgtgtcgcgt gcgccagaac
     1561 gcagaagcgg aacgtatcac cgtcgaagcc atcgagtaca tgtgcgaccg ttctctgacc
     1621 ttgttgcgtc gcggggccgg agcccacttc cgccagattc ccgacgatgg gcgcaccgga
     1681 gtctttctcg acctgcgctt tgccggtgac ccgacggctg cctggcatcg gctggcacaa
     1741 tggctgcgtg agtgcggcag ctcggaagac agctgtctgt cagccatggg cgcggtacat
     1801 ctggcacagg tgaaagagat tcgacacgct ctgccggaaa gcgtcaatca tcgcatttcc
     1861 gagattcgcg ccgtgcatcc gcagttgacc aagctcggta ccgacatggc cgttccggat
     1921 gaacacctca acgaggtgat ggaactctat gccgcacggc tcaccgaagc gggactgcag
     1981 catgtcatct tcgggcacat cggcaacaac catctgcacg tcaatatcct gccggagacg
     2041 ccggctcagt atgaacgggg aaaagcgttg tacaaagagt ttgcggcttc gatcgtgaag
     2101 atgaacggtt cggtcagcgc cgaacatggc atcggaaaac tcaaacgcta tctgctcgag
     2161 tatcagtttt cgaccgcggc tctggcggaa atgaaagcca tcaaggccgc tctcgacccg
     2221 gactttcttc tcggccccga aacactgttc tgagcgtcac gtctcggctg catgtctgaa
     2281 aaagcagatc gggcctgttt ccaggcccga tctgcttttt cggtaacgac cgattctatt
     2341 cgacgttgag gtggatgacc gtcgagccgt cacccatgcc ttcgaccttg atgaccttca
     2401 tgatgccgct cttcgcggtc ttatactgtt cctgcagcat caacaggtct ttgtcggtct
     2461 tcgcgacatt ggtcccgaac atcttcaact cggatgtgta ccgcgccatc atgcggcgca
     2521 ggtcttcgat atcgctcgcc agcaggttgc tcttgcgaat cgtgcgcacc atgttgggcg
     2581 actgggcgat ttcgagcacg gcatcgatgg ttttctgcag catcgaaacc gagatgtact
     2641 tgtcgagctg cttggttttc agataggtaa tcgaactgat tcccttaagc cagcccttgt
     2701 cgacgaactt cagcagaata tcgcggagtt catcggtgtt ggcaactttg gggagggggt
     2761 tggcatccca cgaatagaga gcgctgcgcg aaaccatctt cggtcgcgcc tgctgcgtga
     2821 tcggctgcgt gttcggggga atacgcaacg acagcgtcac ttccttgctc gacatcgatt
     2881 tgtcaggaat cgggatcgtc aaggtggaat cggcgttgac gctgaatgcg aacatggcgg
     2941 caaagccgta gatggcaccg gcaagcagta cgaggcgttt catacgcacc tccagaggaa
     3001 cgatggaaaa tctccgttcc tatcgacagg aagcgcgaat tc
//
LOCUS       DAORHD010000534         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37184_length_3040_cov_3.008040, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000534 DAORHD010000000
VERSION     DAORHD010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37184_length_3040_cov_3.008040"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="PKO06_11670"
     CDS             <1..1603
                     /locus_tag="PKO06_11670"
                     /inference="COORDINATES: protein motif:HMM:NF021033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TM1802 family CRISPR-associated protein"
                     /protein_id="HNV70349.1"
                     /translation="SEKPWGSYFREITSNLARPRLRLPDQAEITWKDHDYGSSLEAAV
                     DRIDARQTIFLAVADSLGKLPGERLEYCSYLQEEVLSGDRYVTGAASAHPKATCPLCH
                     VIDVPVYPNALKGAGINLANADRAGAFPGINIDNAWKGYSLCKPCSDLLFIYKFHVLK
                     EDESLQRRPFTNRIAGEQALIVPHFSARSSEWRPLLKMFRDVVKTARTDVELDEDDML
                     DRLREQRALLSVDFLWADLGQSIENLRGMISDVPPTRLRFLSTLNAAQTRIKHAVFPE
                     RHLPSLRPTLGLNALKDLFKLPGGKKTQKENEGPGSFHLRRQIASCVYHNLPLLWSRL
                     SEAIIITARAYWDEVTISGNWEQALFEGFNKKKNEGYLTTAGWVKHLAWWLYYFKRLG
                     VLPMEKNVHTPQLETLKPYFGPESGIDSPEKAFAFLLGTAYGYLLRVQAARGVNVSSN
                     ALTWLKRLTLKGRDLPSLYIKIREKLLIYEAEKRQDLRQLLEEIGQLGIRLGTNIQLD
                     EVATNYYLLLGQSVANNVFPSAGKE"
     gene            1622..2575
                     /gene="cas7b"
                     /locus_tag="PKO06_11675"
     CDS             1622..2575
                     /gene="cas7b"
                     /locus_tag="PKO06_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas7/Csh2"
                     /protein_id="HNV70350.1"
                     /translation="MTTAPQTLSQRHEFLFLYDIKMGNPNGDPDENRPRVLPDGTHYV
                     TDVRLKRFARDFFKVRNRDILVDSIEGRTTNLTGRVVEHLKKVNKPKADGAELVSILL
                     DAFIDARLFGSSLAFKKLDKYEPSPEPKTLTGAVQINMGEVLHPAEEVDIRGTSVFAS
                     QEDNKQGTFTTYFGLRYALIGFSGIANEHSAKLSRLTDGDYHELLQAIWNGVRSVGNT
                     RTKRGQMPHLLIDVAYKPGVEFQYGRLHDYVRLSSREKPEPKTWAGPQDYEVDLSLLQ
                     KRLSEKESLIEKVRVMKSSDLQLKSPFSLPSEEAINLELAL"
     gene            2579..>3040
                     /gene="cas5"
                     /locus_tag="PKO06_11680"
     CDS             2579..>3040
                     /gene="cas5"
                     /locus_tag="PKO06_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HNV70351.1"
                     /translation="MKLTRFKLSGRIGHYLRAEANASAPSYPVPPRTALVGLVGAVLG
                     LEKDRPQVVLQTAGVAVSGVLPQTHWHKAKLRKDPPENLPWTIRSNQKTDKQTKEERT
                     GLVTQEWLVNPIYTVWVHLPESFHGQFEERLRESRWHFTPCLGLSEMLADLV"
BASE COUNT          742 a          863 c          756 g          679 t
ORIGIN      
        1 aagcgaaaaa ccctggggtt cttatttccg tgaaattact tctaatctgg cgcgaccacg
       61 gcttcgctta cccgaccaag ccgagatcac ctggaaagac catgactacg gctcgtccct
      121 ggaagcggca gtagacagaa tcgatgctcg acaaacgatc tttcttgcgg tggcagactc
      181 tcttggaaaa ttgccgggag agaggctgga gtattgctcc tacctgcagg aagaggtgtt
      241 gtcaggcgat cgttacgtta caggcgcagc cagtgcacac cccaaagcga cgtgcccatt
      301 gtgccatgtc atcgatgtac ccgtgtatcc aaacgcactc aaaggagccg gtatcaatct
      361 cgcgaacgca gatcgagccg gagcatttcc cggcatcaat atcgacaatg cctggaaagg
      421 atattcgttg tgcaagccgt gttctgacct tcttttcatc tacaagtttc atgtcctcaa
      481 ggaagacgaa agtctccaac gtcgaccctt cacgaaccgc attgctggtg aacaggcgtt
      541 aatcgttccg catttcagtg ctcgttcttc agaatggcgc cctctgctga agatgtttcg
      601 cgacgtcgtg aagaccgctc gcaccgatgt cgagcttgac gaagacgaca tgctcgatag
      661 acttcgcgag caacgcgccc tgctttctgt cgactttctc tgggccgacc tcggccagtc
      721 catcgagaat ctacgcggta tgatttcgga cgtccccccg acacgcttgc gctttttgtc
      781 cactttgaac gccgcgcaaa cgcgcatcaa acacgccgtg ttccccgagc gacatttacc
      841 gtccttgcgc cccaccctcg gcttgaatgc tctgaaagat ctgttcaagc tccccggtgg
      901 gaagaagacg caaaaagaaa acgaaggccc aggttcgttt cacctgcgtc ggcagatagc
      961 atcttgtgtt tatcacaatc tgccgttgct gtggtcgcgg ttgtctgaag caatcatcat
     1021 cacagcccga gcctactggg atgaagtcac catctccggt aactgggaac aagcattgtt
     1081 tgagggtttc aacaaaaaga agaatgaagg ctatctcacc accgcaggct gggtcaaaca
     1141 tctcgcctgg tggctctatt acttcaagcg actgggagtc ttacccatgg aaaaaaacgt
     1201 gcacaccccg caactcgaaa cgctgaagcc gtatttcggc ccggaaagcg gcatcgattc
     1261 gccagagaaa gcttttgctt ttctccttgg gacggcatac gggtatctcc tgagagtcca
     1321 ggccgctcgt ggcgtcaatg tttcatccaa cgctcttaca tggctcaaga gattgacgct
     1381 caagggtcga gatcttccca gtctgtatat caaaattcgc gaaaaacttc ttatctatga
     1441 agcagaaaag cgtcaggatc tacgccagct acttgaggaa atcggacaac tgggcattcg
     1501 actgggcacg aacattcagc ttgacgaagt ggcgacgaac tactatctgc ttctcggaca
     1561 atcagtcgca aacaacgtat ttccttctgc aggaaaagaa taacctttcg gaggattccc
     1621 tatgactacg gctccacaaa ccctctcgca gcgtcatgaa ttcctgtttt tgtatgacat
     1681 caaaatgggc aatcccaacg gggacccgga cgaaaaccgc ccgcgcgtcc tccccgacgg
     1741 tacgcattat gttacagatg ttcgcctgaa acgctttgct cgcgactttt tcaaagttcg
     1801 aaaccgcgac attctcgtcg actcgatcga aggtcgcacc acgaatctga ctggtcgggt
     1861 cgtcgagcac ctcaagaaag tcaacaaacc gaaggccgac ggtgccgaac tagtcagtat
     1921 tctcctcgac gctttcattg atgcccgatt gttcggcagt tcgctggcat tcaagaaact
     1981 agacaaatac gagccctcac ccgaaccgaa aaccctcacc ggggcggttc agatcaatat
     2041 gggtgaagtc ctgcacccgg ccgaggaggt cgacattcgt ggcacatcgg tcttcgcaag
     2101 ccaggaggac aacaagcagg gaaccttcac gacctatttc ggcctgcggt atgcccttat
     2161 cggcttttcc ggtatcgcca acgaacattc agcgaagctt tcgcgactga ccgacggtga
     2221 ttaccatgag cttctgcaag ccatctggaa cggagtccgc tctgtcggca acactcgcac
     2281 caagcgaggg cagatgccgc accttctcat tgatgtcgct tacaaacccg gcgtcgaatt
     2341 tcagtacggt cgcctgcatg attatgtccg actcagttcg cgcgaaaagc ctgaaccgaa
     2401 aacctgggcc gggccgcagg attatgaagt cgatctttct ctcctgcaga agcgtctcag
     2461 tgagaaagaa tccctgatcg aaaaagtacg agtgatgaag tcttcagacc tgcaactcaa
     2521 atcgcctttc agcttgccaa gcgaagaggc aatcaacctg gaacttgcct tgtagtccat
     2581 gaaacttaca cggttcaaac tttcgggccg tatcggtcat tacctgcgtg ccgaggccaa
     2641 tgccagcgca ccgtcctatc ctgttccgcc gcgaacggca ttagtcggcc tcgtaggtgc
     2701 cgtactcggt ctggagaagg atcgacctca agttgtcctt cagaccgcgg gggtcgccgt
     2761 ctcaggagtt ttaccgcaaa ctcattggca caaagccaag ctccgaaagg accctccgga
     2821 aaaccttccc tggacgataa gaagcaatca aaaaacggac aagcaaacga aggaagaacg
     2881 taccggtctg gtcactcagg aatggctggt gaacccgata tacacggtct gggttcattt
     2941 gcccgagagt tttcacggcc agttcgaaga gcgcttgcgt gagagccgct ggcatttcac
     3001 cccctgcctg ggtctttcag agatgctcgc tgatctcgtc
//
LOCUS       DAORHD010000535         3037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37245_length_3037_cov_3.636821, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000535 DAORHD010000000
VERSION     DAORHD010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37245_length_3037_cov_3.636821"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2389)
                     /locus_tag="PKO06_11685"
     CDS             complement(<1..2389)
                     /locus_tag="PKO06_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV70352.1"
                     /translation="MISAHVFRRLAAWAAFILFVVLPIGAPVWVWDRLWREHDSDLRR
                     QAEATLERDLQLMQFDSAPQQMLGRSFDRCFQYLSTHGFSSTAVQCFPQLVHPELHAR
                     VVVFTTDGKLEVPPWSSAEARRIIQQLWGDILDRTNWPPETRQRMYKALFGPAFFIGD
                     LRNSEGFFYNIGSGQNDGVLLWKKGPAKSGLLMYIANFPGAYTLLRRQMLDTANQSFE
                     EETGFWLILDEPTRRFVTGGAPFPGWLKLFRRERLGEGQIFSHDEYLGKLTRHQNGPW
                     MMRLIHLPRRDTQLYRFLGLLVGLLSICIASPLWWQRGLEFLDTLRISPRLLLFLCLA
                     VLLPSVMLLILSIGAFRERATVLEDQTAEAAIRRLRTADDAIERANAKTLGLYRQLAD
                     HPVVQGSDSAAIARVISPLRDDLRMARFDSHNDRNQVLHTLEQTKKISLLTTEMMGRE
                     VLSRYLGVTFPTRSGSLDTIAPQFLRSTQLGFARIYDHPGELNDLANAGESGVWFWDF
                     RRPTPERPMAFISIMVFSRWILENFLKEDIPSGVWIFDNQFRRWSPGTPPGPLPDRIV
                     AQAFIGKRSVQEIMTVNGREILGIALPSTLIPKYCFYTLADLGPVRRQVQQLRGSLLS
                     GAVLLLLLALIFSRILSRTILVPVVETTRGIEALENRQFTFRLPDLGSDEFGRLGVTF
                     NSMMEDLRDLDMGREVQASLIPLTPPTIPGYELAISYVSAADLGGDYCDAIPTSGGKT
                     LLIIGDVTGHGVAAALLTAIAKAICFLSAREGKTIPETLNRLNKIINVVLKKKKLM"
     gene            complement(2386..>3037)
                     /locus_tag="PKO06_11690"
     CDS             complement(2386..>3037)
                     /locus_tag="PKO06_11690"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV70353.1"
                     /translation="NDHASAFMIGDVSGHGVSASLVMAMAKARVFSHFDEGGTESGLL
                     EQLNDLLFHLTSRRQLMTFCLVLVDFSTHTGTITAAGNPLPVFLRRSDTSVRHLGAIA
                     YPLAVREQQRFEPTPFTFAPGDLLLLYTDGVVEAIDSEQRPYGYDRLQRLMQQTADLG
                     VHGLVQYLQADLGHHLGSCLPLDDITVLALGRVADNHPCTSQTIIIDSPDKESHQA"
BASE COUNT          681 a          854 c          935 g          567 t
ORIGIN      
        1 tcatcagttt cttcttcttc aacaccacat tgatgatctt gttcagtcgg ttcaacgtct
       61 cgggaatcgt cttgccctcg cgggcactga gaaagcagat cgccttggca atggcggtta
      121 acagggctgc agcgacgccg tgaccggtca catcaccgat gatcaacagg gtttttccgc
      181 ccgaagtggg aatggcatcg cagtagtcac cgccaagatc agcggcgctg acataggaaa
      241 tcgccagttc ataccccgga atggtcggcg gagtcagggg aatcaggctc gcctgcactt
      301 ctcgtcccat atcgaggtca cgcaggtctt ccatcatcga gttgaaggtc acgcccagac
      361 gaccgaattc gtccgagcca agatccggca atcggaaggt gaattgccga ttctcgaggg
      421 cttcgatgcc tctcgtcgtc tcgacgaccg ggacgagaat cgtgcgcgac agaatgcgcg
      481 aaaagatcag ggccagcagc agcaacaaca cggcgcccga caaaagactg ccgcgtagct
      541 gctgcacctg gcgtcgcacg ggcccgagat cagcgagcgt gtagaaacag tatttcggga
      601 tcagcgtgct gggcagggca atccccagaa tctcgcgtcc attgacggtc atgatctctt
      661 gaaccgaacg cttgccgata aaagcctggg ccacgatgcg atccggcagt ggtcctggag
      721 gtgtgcccgg agaccagcgt ctgaactgat tgtcaaagat ccagaccccg gacggaatat
      781 cttccttgag aaagttctcg aggatccagc gactgaagac catgatcgag atgaacgcca
      841 tcggtcgttc cggcgtcggt cgccgaaaat cccagaacca cacccccgat tcgccggcat
      901 tggcgagatc gttcaactcg ccgggatgat cgtagatgcg ggcgaaccca agctgtgtac
      961 tgcgcagaaa ctgcggcgcg atcgtatcga gactgccgct gcgcgtcgga aaggtcactc
     1021 cgagatagcg cgaaagcact tcccggccca tcatttcggt ggtcaagaga gaaatcttct
     1081 ttgtctgctc gagggtgtgc agaacctgat tccggtcatt gtgcgagtcg aaacgggcca
     1141 tgcgcagatc gtcacgcagt ggggaaatga cccgggcgat cgccgcactg tcggagccct
     1201 gcaccacggg gtggtcggcg agttgccgat aaagccccag cgttttcgca ttcgctcttt
     1261 cgatggcatc gtcagcggtg cgcaggcgtc gaatcgcggc ttcggcagtc tgatcctcga
     1321 gaaccgtggc gcgttcgcgg aatgcgccaa tactgaggat cagcagcatg accgagggca
     1381 gcaggacggc gagacacaaa aacagcagca ggcgcggcga aatgcgcaag gtgtcgagga
     1441 actcgagacc gcgttgccac cacaacggac tggcgataca gatgctcagc aggccgacga
     1501 gaaggccgag aaaccggtac agttgtgtgt ctcgtcgcgg cagatgaatc aggcgcatca
     1561 tccacggccc gttctgatgg cgagtcaact tgccgagata ctcgtcgtgc gaaaagatct
     1621 gaccttcgcc cagccgttcc cggcgaaaga gtttcagcca gccgggaaag ggagcgccac
     1681 cggtcacgaa tcgacgggtc ggttcatcga gaatcagcca gaacccggtt tcctcctcga
     1741 acgactggtt ggcggtatcg agcatctggc gccggagaag ggtatatgcg cccggaaagt
     1801 tggcaatgta catcagcagt ccgctttttg cggggccttt cttccagagc agcacgccgt
     1861 cgttctggcc gctgccgatg ttgtagaaaa agccttccga gttgcgcaga tcaccgatga
     1921 aaaacgccgg accgaaaagc gctttgtaca tgcgctgccg ggtttccggg ggccagttcg
     1981 tacggtcgag gatgtctccc cagagctgct gaatgatacg cctcgcctcg gcagagctcc
     2041 agggcggcac ttccagtttg ccgtcggtgg tgaaaaccac gacacgggcg tgcagctccg
     2101 gatgcacgag ttgtggaaaa cattgcacgg cggtggagga aaatccatgc gtggacagat
     2161 actgaaaaca gcgatcaaaa gaacgcccca gcatctgctg cggcgctgaa tcgaactgca
     2221 tcagttgcag atctcgttcg agcgttgctt cggcctgacg tcgcagatca ctgtcgtgtt
     2281 ctcgccacag ccggtcccag acccagaccg gggctccgat gggtaggacg acaaacagga
     2341 taaaggcggc ccatgcggca agtcttcgaa acacgtgggc agaaatcatg cctggtggct
     2401 ctccttatct ggagagtcta tgatgatcgt ctggcttgta catgggtggt tgtcggcaac
     2461 tcggcccaga gccaacacgg tgatgtcgtc gagtggcagg cagctgccga gatgatgtcc
     2521 aaggtcggct tgcaggtatt gtaccagtcc gtgaacgcca agatcggctg tctgctgcat
     2581 gagacgctga agacgatcgt aaccataggg gcgctgctcg gaatcaatgg cttcgacgac
     2641 gccgtcggtg tagagcagca gcagatcacc gggtgcaaaa gtgaatggtg tgggttcgaa
     2701 acgctgctgt tcgcgtaccg cgaggggata cgcaatcgcg ccgagatggc ggaccgacgt
     2761 atcggaccgg cgcagaaaga ccggcagcgg atttccagcc gcggtaatgg tccccgtgtg
     2821 ggtcgaaaag tccacgagca ccaggcagaa ggtcatcagc tgacggcgag aggtcagatg
     2881 gaacagcaga tcgttcagct gttcgagcag cccgctttcg gtgccccctt cgtcgaaatg
     2941 cgaaaacacg cgggccttcg ccatggccat gaccagcgaa gcgctgacgc cgtgacccga
     3001 gacatcgccg atcatgaagg ccgaagcgtg atcgttg
//
LOCUS       DAORHD010000536         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37321_length_3033_cov_3.406313, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000536 DAORHD010000000
VERSION     DAORHD010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37321_length_3033_cov_3.406313"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1131)
                     /gene="aroC"
                     /locus_tag="PKO06_11695"
     CDS             complement(64..1131)
                     /gene="aroC"
                     /locus_tag="PKO06_11695"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV70354.1"
                     /translation="MSNSFGKFFRVTTWGESHGPALGAVIDGCPAGLSVEDDDIREQL
                     DRDVPDRELGTPRGEINRFQILSGVFEGKTLGTPISIIVWNNDARPRTYSGGRDTLRP
                     GHADFTWLARYGHTDWRGGSRASGRECIARLVAGAVARKLAASVGVRLTGRVVELAGL
                     PVTNEQDYAVAKLRALEIAGQGDSTGGVIEVLIEGMPPGVGNPVFGKLQADLAAAFLS
                     IGGVKGLETGEGFAAARMRGSLHNDPFALDEDGRVVSVSNRCGGLLGGISTGDRMSFR
                     LAVKPTPTIQRPQQTVNVVEGKPVCMEYNGRFDANYAPRVVPIAEAMAACVITDHLIC
                     SGFMNPARHGSVEPITVEDEL"
     gene            1212..3014
                     /locus_tag="PKO06_11700"
     CDS             1212..3014
                     /locus_tag="PKO06_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70355.1"
                     /translation="MDRYFVLLFSFGFVGLVIFDLLRQRTAEDKTLSSRLKTDDPEPA
                     AADPIRTAHLESQRLEALSLIERLEAAATIEETDRVQLRQLLLSRNPEIESRAFDLLA
                     RDPLEMPFLLGYLKSQEATLMKMIDEKQRFTVQVASFDQFFKTKNLKMLSRLSLTRPK
                     QLSPLASVWKIDLGVARRMLEGILAKDNTPGIHDPEADGVIKPGRRELLPRLEQIVDA
                     REQETPVRLSAVLAIGEIDPVHGWEILVRALESDQPVLRFCAVETIGNLGRLEALPLL
                     RHACRDQHPMIRSVALDGLARLGDEDCLPLIVEYLADSHSEVAFSARQALIRLKCRAE
                     VCDYLLENIPRLPAPAAAATFEILGQIGERRVIPILEAALERDTPELALAAANGLLML
                     RETNHLDRILTLADKLGAETRDLATTLEQDTDQKRLDQFLADKMQQCTDPDVQFFAIQ
                     ILLNEMSGKMNDVLRDSIEKAAVSTNSFVRAAAMTALARLKSASSQELLEKGLKDTIP
                     YVRACAVSALYQRFRETALDQLCSQLSTEPEEDVLYAILACLYQSGSSPHLHRLEQLA
                     HLLPAPVAAFAQQILALRSTPNPVKSVVTLSESP"
BASE COUNT          669 a          945 c          780 g          639 t
ORIGIN      
        1 tagcggcctt cagcgtagcc aggggagaga catcccggca cagaaaccgc cgcgcgaaat
       61 cactcagagc tcgtcttcaa cggttatcgg ttcaacgctg ccatggcggg ccggattcat
      121 gaatccgctg cagatcaggt gatctgtaat cacacaggcc gccatcgctt ccgcaatcgg
      181 aacaacccga ggcgcgtagt tcgcgtcgaa gcgaccgttg tattccatgc acacgggttt
      241 gccttcgacc acattcaccg tctgttgcgg acgctgaatg gtgggagtcg gtttcacggc
      301 cagacgaaaa ctcatgcgat caccggtgct gattcctccc agaaggccac cacaccgatt
      361 actgacactg accactcgcc catcttcgtc gagcgcaaat ggatcgttgt gtagcgaacc
      421 tcgcatgcga gcggcagcaa aaccctcacc tgtctccagt cccttcacac caccaatcga
      481 cagaaatgcc gcagccaggt cggcctgtaa cttgccgaag acagggtttc caactcccgg
      541 cggcatcccc tcgatcagaa cctcaatcac tccgcctgta ctgtcaccct ggccggcgat
      601 ctcaagcgcg cgcagtttgg cgacagcgta atcctgctcg ttggtcaccg gcaaacccgc
      661 gagttcaaca actctaccgg taaggcgcac gccaactgaa gccgcaagtt tccgtgcgac
      721 cgcaccagcc accagccggg cgatgcattc gcgcccactg gcacgcgaac cgccgcgcca
      781 gtcagtgtga ccataccgcg ccagccacgt gaaatcggca tgtccggggc gaagagtatc
      841 acgtccgcca ctataggtgc gaggccgtgc atcattgttc cagacaatga tcgagatcgg
      901 ggttcccagt gttttgccct cgaagactcc ggacagaatc tgaaaacgat tgatctcccc
      961 gcgcggtgtt cccagttcgc gatcagggac gtcgcgatcg agttgttcgc gaatatcatc
     1021 atcttcgaca gacagacccg ctggacaacc gtcaataacc gctcccaggg ctggtccatg
     1081 cgactcgccc catgtcgtta cgcggaagaa tttaccaaaa ctgttgctca tactggcgtg
     1141 agtataccga atgacgcctt cccttgtata caacatggac cattccccgg attctgccga
     1201 tgacaagaga gatggatcgc tattttgttc ttctcttcag cttcggattc gtggggctgg
     1261 tcatcttcga tctgctgcgc caacggactg cggaagacaa gaccctgtcg tcacggttga
     1321 aaaccgatga ccccgaacca gccgcggccg atccgatcag aaccgcgcat cttgagtccc
     1381 agcgactcga ggctctctct ctcatcgaaa gactagaagc ggcagcaaca attgaagaaa
     1441 ctgatcgcgt tcagcttcgt cagctgttac tgagtcgcaa tcccgaaatt gagagccgcg
     1501 cctttgatct gctggcacgc gatccgctgg aaatgccatt cctgctcggg tatctgaagt
     1561 cgcaggaagc gactctgatg aaaatgatcg atgagaaaca gcgtttcacc gttcaggtag
     1621 cgtcattcga tcagttcttc aaaaccaaga atctcaagat gctctcccgt ttgagcctga
     1681 ctcgccccaa acagttatcc cccctcgcca gcgtctggaa aatcgatctc ggtgtcgcgc
     1741 gacgcatgct cgaaggaatt ctggccaaag acaatacccc agggatccat gatcctgaag
     1801 ccgacggagt gatcaaaccc ggacgccgtg agcttctgcc acgtctcgaa cagattgtcg
     1861 acgccagaga acaggaaact cccgtacgac tttcagcggt actggccatt ggcgagattg
     1921 atccggttca tggctgggag attctggttc gtgcgctcga aagcgatcag ccggtcctgc
     1981 gcttctgcgc ggtcgaaacg atcggcaatc tcggtcgtct ggaagccctg cccctgttgc
     2041 gacacgcctg tcgtgatcaa catcccatga ttcgcagcgt cgctctcgac gggctcgcgc
     2101 gcctgggcga tgaagactgc cttccgctca tcgtcgagta tctcgccgat tcacacagtg
     2161 aagtggcgtt ttcggcgcgc caggcactca ttcgtctcaa gtgtcgtgcc gaggtctgcg
     2221 actatcttct cgaaaacatt cctcgattac cagctccagc agctgcggca acgtttgaaa
     2281 tactcggaca aattggcgaa cggcgggtga ttcccattct ggaagcagct cttgaacgtg
     2341 acacccccga actggcgttg gcagctgcaa acgggttgtt gatgctacgc gagaccaatc
     2401 acctggatcg cattctcacc ctcgccgata agctcggtgc cgaaacccgc gatctggcga
     2461 ccacgcttga acaggatacc gatcagaaac gcctggatca gtttttggcc gacaagatgc
     2521 agcagtgcac cgatccggac gtacaatttt tcgccatcca gattctgctg aatgagatga
     2581 gcggcaagat gaacgatgtg ctgcgcgact cgatcgaaaa ggcggcagtc tcaaccaaca
     2641 gtttcgtgcg tgccgctgcc atgacggctc tcgcccgctt gaaatccgcg tccagccagg
     2701 aactgctcga aaaggggttg aaagacacaa ttccgtatgt gcgcgcctgt gcggtttcag
     2761 ctctctatca gcgctttcgt gaaacggccc tcgatcaatt gtgcagccaa ctctcgaccg
     2821 aaccagaaga agatgtgctg tatgccatcc tcgcctgcct gtaccagtca ggttcttcac
     2881 cccacttgca tcgacttgag caactcgccc atctcctgcc ggcacccgtt gcggcttttg
     2941 cccagcagat tctcgctctc cgttcgacac cgaacccggt caagtccgtc gttacgctct
     3001 cagagagccc ctgatcttcg tctcacccga tcg
//
LOCUS       DAORHD010000537         3032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37334_length_3032_cov_3.179375, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000537 DAORHD010000000
VERSION     DAORHD010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37334_length_3032_cov_3.179375"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1521
                     /locus_tag="PKO06_11705"
     CDS             319..1521
                     /locus_tag="PKO06_11705"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HNV70356.1"
                     /translation="MKKVYVGVTQHPCTENATNSNLLKRWFAANGWEVTRHAPEADIV
                     IISTCGFSKSSEDYEIDEIRRRMADKKSGAELIVVGCLPEINKERLRKEFNGVCISTK
                     DIMKFNEHMNFSTRLEDLDNNFISTDEYEANPHIAFWVKARHRIEQWQKKIPFLRVPR
                     VLLTTPSPSWFCIRCATGCTGNCSYCGIRHAQGFIESDPIDKIVRQAEKGLALGYKEI
                     ALTGEDLGGYGIDRKQDLADLLNELVKLRGDFTINIRFIDPYWLLKLFDKLQPSFNTG
                     KVKSFCVPAQSGSDRILKAMNRRYTFSQIKDGVNDTLRHTKVQMIATNIIVGFPGETE
                     ADFRDSMRLIKEVDFSLYLIFPYEERPETKAARLPNKVPAEIRDRRYERLHRMATKKQ
                     IRHFIGLQ"
     gene            1526..2767
                     /locus_tag="PKO06_11710"
     CDS             1526..2767
                     /locus_tag="PKO06_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70357.1"
                     /translation="MNGGKRFFISNSAHPCTEIALDANRIRLFYLANGWQETVTPGEA
                     DDIIVSTCAVNQQYEDAAMQDLQQRQSQRKPGSRLISTGCLSRINPQRISPLPNLLEV
                     PPLELERFNELVPGNHRMQDVDGHAVRLDDYQANPLFARLVRIRGKLEQLGDQFGIRL
                     VPHWMATIPSRNWYFIRGSTGCLGHCTYCAVRRAKGRLRSTPLEVILQQLRQAVAAGA
                     REISLAGDDMGAWGTDIGSNLAELLSHMISVPGDFRINLRFVEPVFFLRFLDALLPVF
                     ATGRVSAFCLPLQSGSNHVLERMGRDYRIEEVEAGLARFATVANRPRLASIIMVGFPG
                     ETESDYFASCRLLDRLPIDLYQVLMYEGRPGTPSIAMSNQIPTAVKQERHDRLLRKFK
                     LQKVVGIPASLANRLAGLSLS"
BASE COUNT          653 a          939 c          820 g          620 t
ORIGIN      
        1 gctcacaagt ttcgctcttg cgaacggaaa atgggcgaga attaaatgac cctgcccgca
       61 aatcagggtc tcttcactct gtcccatttt cctcctggcg taagcgaaac gtgtgagcct
      121 gccaggcgaa aacctcaggc gcgagtatga catgaccctg gccgcaagtg agcgctactt
      181 gttcgcgcag actcggagcg ggttattgcc tcgcaggcca acttgcgtta ccatgtcatc
      241 gctggcgaca acggccttca catcaactcg accccgtgac gcatccgttg cgcaagtcaa
      301 tccaggagca caacccgcat gaaaaaagtc tatgtcggcg tcacccagca tccctgcacc
      361 gaaaatgcga cgaacagcaa cctgctcaaa cgctggttcg ccgccaatgg ctgggaagtc
      421 acccgccacg ctccggaagc agatatcgtc attatttcga cctgcggttt cagcaaaagc
      481 tctgaagact atgagatcga cgaaatcaga cgccgcatgg ccgacaagaa gtcaggcgca
      541 gaactcatcg tggtcgggtg tttgcccgag atcaacaaag aacgcctgcg caaggagttc
      601 aacggggtct gcatctcgac caaggacatc atgaagttca acgaacacat gaacttctcg
      661 acgcgtctcg aagatctcga caacaatttc atctcgaccg acgagtacga agcgaatccc
      721 catatcgcgt tctgggtgaa agctcggcat cgcatcgagc agtggcagaa gaagattccc
      781 tttttgcgtg ttccgcgggt actgctcacc actccttcgc caagctggtt ctgtattcgc
      841 tgtgccaccg gctgcacggg aaactgcagt tactgcggca ttcgtcacgc ccaggggttc
      901 atcgaaagcg acccgattga caagatcgtg cgtcaggccg agaagggcct ggcactcggc
      961 tacaaagaaa ttgccctgac cggcgaagat ctcggtggct atggcatcga tcgcaagcag
     1021 gatctcgccg atctgctgaa cgagttggtc aagctgcgcg gcgatttcac gatcaacatt
     1081 cggttcattg atccctactg gctgttgaag ctgttcgaca aactgcaacc ctccttcaac
     1141 accgggaagg tcaaaagctt ctgtgtccca gcacaatcgg gcagtgatcg cattctcaag
     1201 gccatgaacc gccgttacac cttttcgcag atcaaggacg gcgtgaacga taccctgcgc
     1261 cacacaaaag tacagatgat cgccaccaac atcatcgtcg gatttcccgg cgaaaccgaa
     1321 gctgatttcc gcgacagcat gcgcctcatc aaagaagtcg atttttcgct gtatctgatc
     1381 ttcccctacg aggaacgacc ggaaaccaag gcggccagac ttccgaacaa ggtgcccgcc
     1441 gagatccgcg atcgccggta tgagcggctg caccgcatgg ccaccaaaaa gcagatacgg
     1501 cacttcatcg ggctgcagta atgccatgaa tggcggaaag cggtttttca tctccaactc
     1561 ggcgcacccg tgcaccgaga ttgcgctcga tgccaaccgc attcgtctgt tctacctggc
     1621 caacggctgg caggaaaccg tgacgcccgg cgaggctgat gacattattg tttccacgtg
     1681 cgcggtcaat caacagtatg aagatgccgc gatgcaggat ctgcaacagc gtcagtccca
     1741 gcgcaaaccc ggcagtcgcc tgatctcgac cgggtgtctc agccgcatca atccgcaacg
     1801 catctccccc cttcccaatc tcctggaagt cccgcccctg gagctcgaac ggttcaacga
     1861 actcgtaccc gggaaccacc gcatgcagga tgtcgacgga cacgcggtcc gtcttgatga
     1921 ttatcaagct aatccgctgt ttgcgcgtct tgtccgaata cggggaaaac tcgagcagct
     1981 gggcgatcaa tttggcattc gcctggtgcc tcactggatg gccacaatac catctcgcaa
     2041 ctggtacttt attcgcggct cgaccggctg cctggggcac tgcacctact gcgcggtgcg
     2101 gcgagccaag gggcgtctgc gcagcacccc gctcgaggtc attcttcaac agctgcggca
     2161 ggccgttgcc gcgggagcac gcgagatctc gctggccggc gacgatatgg gcgcctgggg
     2221 aacggacatc ggcagtaacc tggccgagct gctgagtcac atgatctccg tacccggaga
     2281 ctttcgcatc aatctgcggt tcgtcgaacc cgtctttttt ctcagattcc tcgatgcgct
     2341 gctgccggtg ttcgcgaccg gaagggtttc ggcgttctgt ctgccgctgc agagcggctc
     2401 gaaccacgtg ctcgaacgca tgggtcgcga ctaccgcatc gaggaagtcg aggctggtct
     2461 cgcgcgtttc gccaccgtgg ccaatcgccc acgcctggcc tcgatcatca tggtaggctt
     2521 tcccggcgaa accgagtccg actactttgc cagctgccgt ttgctggacc gcctgcccat
     2581 tgatttgtac caggttctca tgtatgaagg ccgccctgga acaccaagta tagccatgtc
     2641 gaatcagata ccgaccgcgg tcaagcagga gcgtcacgat cgtcttctgc gcaagttcaa
     2701 actgcagaag gtcgtcggaa tccccgcttc actggccaat cgcctggcgg gtctgtctct
     2761 ctcctgacga tgctgcgaca caccctgcgc caactctgga aacgtctgcg cggtctgtgg
     2821 tctctgctgc gaggtcgacc ggcacgcgtt cttccccgtg ccacctattc ggagtgggat
     2881 gacatcgacc ggaaccttct gccatgaccg aaaccctcag gcgcatcgcc ctgctcgaac
     2941 cttccgttgc ttccgaatgc gtggtcacgg agttgatggg ccagattcgc tgcggcagcc
     3001 cctttctggc cggcgctctg gagcagcagg gg
//
LOCUS       DAORHD010000538         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37476_length_3026_cov_3.764726, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000538 DAORHD010000000
VERSION     DAORHD010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37476_length_3026_cov_3.764726"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1749
                     /locus_tag="PKO06_11715"
     CDS             85..1749
                     /locus_tag="PKO06_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70358.1"
                     /translation="MGVLGRTFVKGFRPCLFRNQPVQQFYGHLQAVMKEIDSSGGMEH
                     LFAKPFLPGGDLSQTSEIEWMTDLPGEPRRYRDLPAQEQQRVGAQLMASFEKIRAYAE
                     ARLGKSGIERDYANYLQAVAVSPDLNQVFVVGDRPVILHWGFVSETDQHPGQRMFAGW
                     EEFIGQIQRKAPPAPTPTPAPAAKPAPIPVPPPMPVEPAKPVPVEEKKPVEEKKPVEE
                     KKPVAPPPKPAEQPKPPEERPTMACGLGEYQWVKWLAILLAIIILILLLLRLLPQRNP
                     MSGMMPGSGMSSGGGMPGGGGMPGGGGMPGGGGMPGGSPGGSPGGRQNFPGGVCPSCG
                     THVNDGSGSGSQGAAPGGAAPGSSVTGGSTGGGQNTTGSGQNSTGGNSQTGQHDRSSS
                     STTTSGSSQSGSHQSGSTPGTTPGGTITPGSGQPGESLASGTPVVPPSNSPGSNDGRP
                     ASEGQPGQSSSKAPDISFAEAGDVDRVLISRPSDPGTTWKLVDADGRPFPTTEAFLQK
                     SPGQVNSTGDRVTVSLRPLPGKPLECTVVAVSSSGVTSRYSFVTQK"
     gene            1764..>3026
                     /locus_tag="PKO06_11720"
     CDS             1764..>3026
                     /locus_tag="PKO06_11720"
                     /inference="COORDINATES: protein motif:HMM:NF021642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfC family protein"
                     /protein_id="HNV70359.1"
                     /translation="MSTNPLRPEEQEVIGMAGRLGELLSRGADWANTYLQDAQIRAFM
                     LKNLHEMQITVKRIGESALRPMALGVFGASQCGKSYLVSELVRGTDKTFSVLLSKSGG
                     SPQPHDYLDEINPAGGRESTAVVTRFTARPYRQIAGCAAAVRLHSVTDLVKILLNGFL
                     FECQSDFLPTAEELQKVRFSFRAVSRRNQGNALLSEADVWDLQDYARRHFRNQFVKAL
                     EDIDYWGILQEEIRFLPADQQITYLEWLWGRFPKITQLFRHLVGALGTLGGEIVGISE
                     DALIPRENSIIDVQRLSQLMKVGNRKCTVVTAAGSPVPLDAAVLCALTAELILEVHST
                     QKDSLVQLADVLDFPGARARAQVYDEGRLEKDPASLVEVFLRGKVAYLFDRFSDDRDI
                     TGLMLCQEGGPQEAKSLPYMINKWVEWSM"
BASE COUNT          582 a          889 c          965 g          590 t
ORIGIN      
        1 ggctggggcg tgtctggacc tgatctccgg ggaaacccgg ccggcggcgc gagtgtaccg
       61 tcgagacaac aagggagata aacgatgggc gttctcggca gaacctttgt gaagggcttc
      121 cggccctgtc tgtttcgcaa ccaaccggta cagcagttct atggtcatct gcaggcggtc
      181 atgaaagaga tcgacagcag tggcggcatg gaacacctgt ttgccaagcc gttcctgcct
      241 ggcggtgatt tgagccagac gtcagaaatt gaatggatga ccgatctgcc gggagaacca
      301 cgtcgctacc gggatctccc cgctcaggaa cagcagcgcg tgggcgctca gctcatggct
      361 tcgttcgaga agattcgtgc gtatgccgaa gcccgcctgg gcaaatccgg catcgagcgt
      421 gattacgcga actacctgca ggcggttgcg gtctcccccg atctcaatca ggtctttgtc
      481 gtcggcgatc gccccgtcat tctgcattgg ggcttcgtca gcgagaccga tcaacatccc
      541 ggccagcgca tgtttgccgg gtgggaagag ttcatcggac agattcagcg caaggctccg
      601 ccggctccca ctccgactcc ggcaccggct gccaaacccg ctccgattcc ggtgccgccg
      661 cccatgccgg tcgagccggc gaaaccggtt ccggtcgaag aaaagaaacc ggttgaggag
      721 aaaaagcccg tcgaggaaaa gaagccggtc gctccgccgc ccaaaccggc ggaacagccg
      781 aagccgcctg aagaacgtcc gaccatggcc tgcggtctgg gtgagtatca gtgggtgaaa
      841 tggctggcga ttctgctggc catcatcatc ctgattctgc tgctgttgcg cctgttgccg
      901 caacgcaacc cgatgagcgg gatgatgccc ggcagtggca tgtcatccgg cggcgggatg
      961 cccggaggcg gtggtatgcc gggcggcggt ggcatgcctg gaggcggtgg tatgccaggc
     1021 ggatctcctg gaggctcacc cggtggccgt cagaactttc ccggtggagt ctgtccgagt
     1081 tgcggcacgc atgtcaacga cggctctgga agtggcagcc agggagcggc tccgggtggg
     1141 gctgctccag ggagctcggt gactggcggg tcgaccggcg gcgggcagaa tacgacagga
     1201 agcgggcaga acagcaccgg cggaaactcg cagaccggtc agcatgatcg atcgtccagt
     1261 tccacaacca ccagcggcag ctctcagagc ggaagccatc agtcaggatc cacacctggg
     1321 acaacacccg gtgggacaat cactcctggc tcgggccaac ccggtgaatc gcttgccagc
     1381 ggcactcctg tcgttccccc gagcaacagt cctggttcga atgatggcag accagcttcc
     1441 gaaggccaac ccggtcaatc aagctcgaag gctcctgata tttcgttcgc tgaagctggt
     1501 gatgttgacc gggttctgat ctcccgaccc tcggacccgg gtacgacctg gaagctggtc
     1561 gacgccgatg gacgaccgtt cccgacgacc gaagcctttt tgcagaagtc gcccggtcag
     1621 gtgaactcga cgggcgatcg cgtgactgtg tcattgcgcc cgttgcctgg caagccgctt
     1681 gagtgcaccg tggtcgcggt cagttccagc ggcgtgacct cccgctattc cttcgtgacc
     1741 cagaaataag cgaggtgatc ctcatgtcca cgaatccgtt gcgccccgaa gaacaggaag
     1801 tcatcggtat ggccggacgg cttggcgagc tgctgagccg tggtgccgac tgggcgaaca
     1861 cctatctgca ggatgcgcag attcgcgcgt tcatgctgaa gaatctgcat gagatgcaga
     1921 ttaccgtgaa acgcatcggc gaatcggcgc tgcgacccat ggctcttggg gtgttcggcg
     1981 ccagtcagtg cggcaagtcg tatctggttt ccgaactggt gcggggaact gacaagacgt
     2041 tttcggtgct gctcagcaag agcggcggct caccgcagcc gcacgattac ctcgatgaga
     2101 tcaatccggc cggtggccgc gagagcacgg cggtcgtcac ccggttcacg gcgcggccgt
     2161 atcgtcagat tgccgggtgc gctgcggcgg tgcgtctgca cagcgtcacc gatctggtga
     2221 agatcctgct gaacgggttc ctgttcgaat gtcagtctga ctttttgccg acagccgaag
     2281 agttgcagaa agtgcggttc tcgtttcggg ccgtctcgcg tcgcaatcag ggcaacgcgc
     2341 tgctctcgga agccgacgtc tgggatctgc aggactacgc gcggcgtcac ttccgcaatc
     2401 agttcgtcaa agcgcttgaa gacattgatt actggggcat tctgcaggaa gagatccgct
     2461 ttctgcctgc cgatcaacag atcacctacc tcgagtggct ctggggacgg ttcccgaaga
     2521 tcacgcagtt gttccggcat ctggtcggcg cactgggtac attaggcggc gagatcgtcg
     2581 gcatctccga agatgccttg attccacgcg aaaacagcat cattgatgtg cagcgattgt
     2641 cccagttgat gaaggtggga aatcgcaagt gcaccgtggt aacggcagcc ggttctccgg
     2701 taccgctcga tgcggcggtc ctctgcgctc tgaccgccga gttgattctc gaagttcact
     2761 cgacgcagaa agattcgctc gtgcagctgg ctgacgtgct cgactttccc ggcgcgcgtg
     2821 cccgagcgca ggtctacgac gagggtcggc tcgagaagga tcccgcctct ctcgttgaag
     2881 tattcctgcg tggcaaggtc gcgtatctct tcgaccggtt ttctgatgac cgtgatatca
     2941 ccggcctcat gctgtgccag gaaggaggcc cgcaggaagc gaagtcactg ccctacatga
     3001 tcaacaagtg ggtcgaatgg agcatg
//
LOCUS       DAORHD010000539         3018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37607_length_3018_cov_3.850152, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000539 DAORHD010000000
VERSION     DAORHD010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37607_length_3018_cov_3.850152"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKO06_11725"
     CDS             complement(<1..548)
                     /locus_tag="PKO06_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70360.1"
                     /translation="MEKQWPALDLVKCACVIGMILLHCFYWICTVYGRLIVVPDSVLL
                     PWISRFMIVGFLPLMLPLTAGCALRHQLSPGGESAKLSLSQGLGIAGSSMFLLLLGYL
                     MNVLATGWAAVSSWNVLQFVGCSFVIIAAVLTVAPAPVISVACTGAAVLLIYPWIQAI
                     HPGTTGGLLSLIVLGDPSGFHG"
     gene            complement(560..1744)
                     /locus_tag="PKO06_11730"
     CDS             complement(560..1744)
                     /locus_tag="PKO06_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV70361.1"
                     /translation="MMLSMNLREAMRSLMSSKQRTFLALLGIVIGIASVIAMVSVGTI
                     VQREALRQFVELGTDILRITDLTESRSTPGRTFGMQAAKAMRKASPDIGAMAPYTTSY
                     GYVKYRAVQWSMPVIGCTAEMAGICKLRLSRGRHLTALDGGMLNCVIGEKLTSELSKL
                     GVNDPIGKEILVLGNLCTIVGMLERVPMSDMRPIEVNDGLLVSYSTLAAISGKDQVTN
                     IIARLRVDGDTTRAQMQVRGFASKTFPSIHPVVRSAEEVIAQMNRQMNMMTLLLGVIG
                     SISLIVGGVGVMNVMLVSVTERRREIGVRRAIGAQQRDIQWQFLLEAMLLCTIGGVIG
                     IGFGVGSSWLIARYSEWEFLVSWMAVVLGGGVSIMVGVFFGFYPAIQASRLNPIDALR
                     AT"
     gene            complement(1741..2436)
                     /locus_tag="PKO06_11735"
     CDS             complement(1741..2436)
                     /locus_tag="PKO06_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018139025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV70362.1"
                     /translation="MGTSAPLFHLENIHKSFQIGILKTEVLRGIHLDISPGELTAIVG
                     TSGGGKSTLMNILGLLDTPTSGSYHFDGENVLALDDGALSTIRNRKIGFVFQQFNLLA
                     RLSALENVILPLLYRGDSQWRAEQRGRAMLEKVGMAERMFHRPNELSGGQQQRVAIAR
                     ALVGEPAVILADEPTGALDSKVGQEIMDLFVRLNREERITVILVTHDPRTANQCRRIV
                     RVKDGLVVEEAVA"
     gene            complement(2442..>3018)
                     /locus_tag="PKO06_11740"
     CDS             complement(2442..>3018)
                     /locus_tag="PKO06_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV70363.1"
                     /translation="IMPVSGEEGKQGRKTIEKGVTVELGTVLLAVGNLDGFRVKSLVE
                     ENNIAKLKIGQTVTVSEDAYPEVSFPGRITHISSQASGGGRYGQAATFEVVTVVEPVS
                     PEHRHLVRVGLSATLQVTAYDRPDALVVPLSAVLSLDDGHFVMRRDPTTKTFVRVPIV
                     PGVATLDVVEVRDGLQADDQLAANPGDLTKL"
BASE COUNT          730 a          932 c          768 g          588 t
ORIGIN      
        1 cagccgtgaa atccgctcgg atcgcccaac acgatcagcg acagaaggcc gccagtggtc
       61 cctggatgaa tcgcctgaat ccagggatag atcagcaaca ccgcggctcc ggtgcaagcg
      121 acagagatca cgggtgctgg cgctacggtc agcactgcag cgatgatcac gaaactacac
      181 cccacaaact gcagcacgtt ccatgaggaa actgccgccc agcctgtggc gagcacattc
      241 atgagatatc cgaggagaag aagaaacatg ctgcttccgg ctatcccaag accttgcgac
      301 agggacaatt tcgcagactc gccgccaggc gacaactgat gacgcagggc gcaccctgcc
      361 gtaagcggta acatcaaggg caggaagccc acgatcatga agcgagatat ccatggcagg
      421 aggacactgt caggaactac tatcaaacga ccatagacgg tacaaatcca gtaaaaacag
      481 tgcagcagaa tcatgccgat gacacacgcg catttgacca gatcgagagc cggccactgt
      541 ttttccatcg cgtgcgctct caggtggctc gtaacgcatc aatgggattc agtcgtgacg
      601 cctgaatggc cggataaaac ccaaaaaaca ctccgaccat aatcgacact cctccaccca
      661 gcacaacagc catccacgag accaggaatt cccattcgga gtagcgagcg atcaaccagg
      721 acgaaccaac cccaaaacca attccgatga cacctccgat cgtacacaac agcatggcct
      781 cgagcagaaa ctgccattga atgtcgcgtt gctgcgcccc aatcgcacgc cggacgccga
      841 tttcgcgtcg tcgttcggtg accgacacca gcatgacgtt catgacgccg actccgccaa
      901 cgatgagaga aatgctgcca atgacgccca gcaacagcgt catcatgttc atctgccgat
      961 tcatctgcgc gatcacttct tcggcacttc tgacgaccgg atgtatggag gggaaagttt
     1021 tggaggcaaa gccgcgaacc tgcatctgcg cgcgagtcgt gtcgccatcg acccgcagac
     1081 gggcaatgat gttggtgacc tgatctttgc cactgatggc ggccaaggtc gaatacgata
     1141 ccagaagccc atcattgact tcgatcggtc gcatatcact catcggaacg cgttccagca
     1201 tcccgacaat cgtacacaag tttcccagta ccagtatctc tttgccaatc gggtcgttta
     1261 caccgagttt tgacagctca gaagtaagtt tttccccgat gacgcagtta agcattccgc
     1321 catccagcgc ggtcaggtga cgaccgcgcg acaacctgag tttgcagatt ccggccattt
     1381 cggcggtgca accaatcacg ggcatgctcc attgcacggc acgatacttc acatatccgt
     1441 atgacgtcgt gtagggagcc atggcgccga tatccggaga cgcttttcgc atcgcctttg
     1501 ccgcctgcat gccaaatgta cgtcccgggg tcgaccgcga ctccgtcaaa tccgtgattc
     1561 gcagaatgtc ggtccccaac tcaacaaact ggcgcagcgc ttctcgctgg acaattgtac
     1621 caactgatac catggcaata acagacgcaa taccaatcac aataccaagc aaagccagaa
     1681 acgttctctg cttcgacgac atcagactgc gcatggcttc tcgcaggttc atgctcaaca
     1741 tcatgccacc gcctcctcca cgaccaatcc atctttcacc cgcacgatgc ggcggcactg
     1801 gttcgcggtt cgcgggtcat gcgttaccag aatgaccgtg atccgctctt cccgattgag
     1861 tcgtacgaag agatccatga tttcctgacc gactttggaa tcgagagcgc cggtcggttc
     1921 atcagccaga atgactgccg gctctcccac cagggctcgg gcgattgcga cacgctgttg
     1981 ctgaccgcca gacagctcat tgggacgatg gaacatacgc tcagccatac cgactttttc
     2041 gagcatggcc cgaccgcgtt gctcggctcg ccactgggaa tcgccccgat aaagcaaagg
     2101 caggatgacg ttctccagcg ctgagaggcg cgccaacaaa ttgaactgct gaaagacgaa
     2161 gccgatcttc cgatttcgaa tggtcgacag cgcaccgtca tccagtgcca gaacattttc
     2221 gccatcgaaa tggtacgagc cggatgtcgg ggtgtccaac agtcccagga tgttcatcag
     2281 cgtgctcttt ccaccacccg aggtgccgac gatggcggta agctctccgg gagagatgtc
     2341 gaggtgaatc ccgcgcagaa cctccgtctt cagaattccg atctgaaagc tcttgtggat
     2401 attctcgaga tgaaacagtg gcgcggaggt acccataagc gttacagctt tgtcaggtcg
     2461 cccggattgg ccgccaactg gtcgtctgcc tgaagaccat ctcgcacctc gacgacatcg
     2521 agggtcgcaa cgccgggaac gatcgggaca cgcacaaacg ttttcgttgt cggatcacga
     2581 cgcatgacga agtggccatc gtcgagactc aatacagcgc tcaatggcac caccaaagca
     2641 tcaggtcgat cgtatgccgt tacctgcaat gtcgccgaca atcccacgcg taccagatga
     2701 cgatgctcag gcgaaaccgg ttcgaccacg gtcaccacct caaacgtcgc cgcctgacca
     2761 tatcgcccgc cccccgatgc ctgcgaggaa atgtgcgtaa tacggcccgg gaacgacact
     2821 tcgggatagg catcttcgct gactgtgacg gtctggccga tcttcagctt ggcaatgttg
     2881 ttttcttcca ccagagactt gacccggaat ccgtcgagat tgccaacggc gagcaggacc
     2941 gttcccaact cgactgtgac acccttctcg atggtcttcc gcccctgttt cccctcttca
     3001 ccgctgacgg gcatgatg
//
LOCUS       DAORHD010000540         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37627_length_3017_cov_3.534099, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000540 DAORHD010000000
VERSION     DAORHD010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37627_length_3017_cov_3.534099"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1115)
                     /locus_tag="PKO06_11745"
     CDS             complement(42..1115)
                     /locus_tag="PKO06_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70364.1"
                     /translation="MRLVSPVLTIIFAVLLLQPLTAPAQSASSSAPVAVDVATEPVQI
                     EPPPVSAHPGSLLSLTGLATFVPDLADRAATAWSALASPSPALPASSAGALIASPTPR
                     DPSPPEFWQPREPELIIGATIKPREDVDDEKKKTEITGTLIEEPHYLKRRKTFKRWVL
                     RQKDGNRIPLTANLSLMFALQQVSPASGPVTLHGSWLQSKANEGLRYFTVERIQAAVP
                     EPEVQDVPASGSADLDLEDSAEGLSEEERALACAEAEPEPEQEQDSEPGATAGAEPGA
                     EPESDSDEDEPGKATPTPARLQKQPDPDGVTKPVRIQENLDTVPVSNSPEARAPASSP
                     IAIETKASASQPLNTPLNQGGIR"
     gene            1383..>3017
                     /locus_tag="PKO06_11750"
     CDS             1383..>3017
                     /locus_tag="PKO06_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNV70365.1"
                     /translation="MKELNWRLLVIGAFLCVIATAATPYVTLKLGQSVDLTMGGMFLA
                     AYILANRMSSKELAIELNIIQTMIGMVQGIAFMVVILAAFFYIQTVFGRDIGFAPSYW
                     QMVIWLFISANLGVFMGVVPRNSILKDRSLPWPASKAVLSVAQTLTDPEATTTTKIRR
                     DVLTTSTGISGFLVFLRDGLGVIPSIVGNAALNMAVGLEMAALGIGMLVPLSVGLSGL
                     LGTYVINVFGDKVARHAALSGVATEHYDKCMTLVDTFPSLEGAEKAGALEFLTTHGGK
                     AVEFLQSGSHFKYVVQWMMWPATAMMIAAALTSVIIPVVQNVMSRKSEEGQAEETSLA
                     DEEIPAWWSYAGIAVSVVLLVVLTSTWFQMPVAEVLLAVAIQPVLIVAGLRVLGITGS
                     GPVSLMANATQFLFGLIWPAQIRSNLSAAYISASPQAASENVVPAFWVARRLGGKFTT
                     LILAQLFVIPIAALLTPLTLNMLQHTYGIGLNPGQLSAPTGLKIATLAIVMEKGISFM
                     PHGALTASIIAIFVGIFFELLLAFKTKDEQGNETSRF"
BASE COUNT          534 a          883 c          915 g          685 t
ORIGIN      
        1 tgtgtgaaag aaagggaaac atgtgggtgt gccccgtggg gctagcggat accgccctga
       61 ttcagcgggg tattgagcgg ctgtgatgca gaggctttcg tttcgatcgc gatgggtgaa
      121 gaagccggtg cacgtgcttc gggtgagttc gacacaggaa cggtgtccag gttttcttgg
      181 attcgaacgg gcttcgtcac gccgtcggga tcgggttgct tctgcagtcg agcgggtgtc
      241 ggtgttgctt ttcccggctc atcttcgtcg gagtcagact caggttctgc ccctggctcc
      301 gctccagccg tcgcccctgg ctctgagtct tgctcttgtt ctggctccgg ctccgcctcg
      361 gcacatgcga gagctcgctc ttcttcgctc aagccctcag cagaatcttc cagatcgaga
      421 tctgcgcttc cactggctgg gacatcttgc acctcaggct caggaacggc ggcctggatg
      481 cgttcgaccg tgaaataccg caggccctcg ttggctttgc tctgtaacca gcttccatgc
      541 agggtgaccg gaccgctggc gggggagacc tgctgcaggg cgaacatcaa cgacagattc
      601 gcggtgagag ggatgcggtt gccgtccttc tgacgcaaca cccaccgttt aaaagtcttt
      661 cgccgcttga gatagtgcgg ttcctcgatc agggttccgg tgatctcggt ctttttcttt
      721 tcgtcgtcga catcttcgcg cggtttgatc gtcgcgccaa tgatcagttc gggctcacgc
      781 ggctgccaga actccggcgg tgagggatca cgcggcgtcg ggctggcgat caatgcgccg
      841 gcgctcgagg cgggaagggc aggggagggg ctcgccagag ccgaccaggc agtcgccgcc
      901 cggtcagcca gatcaggaac gaaggtcgcc aacccggtca gggaaagcag cgaacccggg
      961 tgcgcagata ccgggggtgg ttctatctgt accggttctg ttgccacgtc aaccgcgaca
     1021 ggagccgatg acgaggccga ctgagccggt gccgtcaggg gctgtagcag caggacggcg
     1081 aagatgatgg tgagaacggg tgatacaagc cgcatggtgt gggcgcagta tatactctcg
     1141 cgatccagct ttcaatgcct cagccactcc tgcgaccggc ccgttccagg acgaaaaaga
     1201 acgtattccc ccactcgcgt tctatgccat gccgatctcg cgttcgctac tacaatcgtc
     1261 tctcgcgcgg gacgaacttt tctctgtcgt ttgcatgcgt ttttgtggtc aagcgacccg
     1321 caatcgagta acatccttcg gtcatcatca tcttcacgaa acttgcgggg ggaagtacag
     1381 gcatgaaaga actgaactgg cgattgctcg ttatcggcgc gtttctctgt gtgattgcta
     1441 cggcggcgac gccgtatgtc accctgaaac tcggacagag cgttgacctg acgatgggcg
     1501 gcatgttcct ggctgcgtac attctggcga accgcatgag cagtaaggaa ctggctattg
     1561 agctgaacat catacagaca atgatcggta tggttcaggg catcgccttc atggtcgtca
     1621 ttctcgccgc gttcttctac attcaaaccg tgttcgggcg cgacatcggc tttgcgccga
     1681 gctactggca gatggtgatc tggctgttca tttcggccaa tctcggcgtg ttcatgggcg
     1741 tcgtgccgcg taactcgatt ctgaaagacc gctcactgcc ctggccggcc tcgaaggccg
     1801 ttctcagcgt cgcccagacc ttgaccgacc ccgaagctac caccacgacc aagattcgcc
     1861 gcgatgtgct gaccaccagt accgggattt ccgggtttct ggtcttcttg cgcgatggcc
     1921 tcggcgtcat tccgtcgatt gtcggcaacg cggctctcaa catggcggtt ggtctcgaaa
     1981 tggccgcgtt gggcatcggc atgctggttc cgctctctgt gggcctgtct ggtcttctcg
     2041 gcacgtatgt catcaacgtc ttcggcgaca aagttgcccg gcatgcggct ctctcgggcg
     2101 ttgccaccga gcattacgac aagtgcatga ccctggtgga caccttcccc tcgcttgaag
     2161 gggcggaaaa agcgggcgca ctcgagttcc tgaccaccca cggcgggaag gcggtcgaat
     2221 ttctgcagtc tggatcgcac ttcaaatatg tcgttcagtg gatgatgtgg ccggccacgg
     2281 cgatgatgat cgccgctgcg ctcaccagcg tcatcattcc ggtagtccag aatgtgatga
     2341 gtcgcaaatc cgaagagggt caggcagaag aaacatcgct ggctgatgaa gagattcccg
     2401 cctggtggag ctatgcaggc atcgccgtca gcgtggttct gctcgtcgtc ctgaccagca
     2461 cctggttcca gatgccggtc gctgaagtgc tgctggccgt cgcgattcag ccggtcctga
     2521 tcgtcgccgg gctgcgggtt ctggggatca cgggttcggg gccggtttct ctgatggcca
     2581 atgcgaccca gtttctgttc gggttaatct ggcccgcaca gattcgctcg aacctgagcg
     2641 ctgcttacat cagcgcttcg ccgcaggccg cttccgagaa cgtggttccg gcgttctggg
     2701 tcgcccgccg cctgggcgga aaattcacga cgctgatcct ggcacaattg ttcgtcattc
     2761 caatcgccgc gctgctgacg ccgctgacct taaacatgct gcagcatacc tacggcatcg
     2821 gtttgaaccc tggtcagttg tccgctccga ccggtctgaa aatcgccacg ctcgcgatcg
     2881 tcatggaaaa gggcatcagc ttcatgcccc acggtgccct gacggcttcg atcattgcca
     2941 tcttcgtcgg catcttcttt gagctgctgc tggccttcaa gaccaaagac gagcagggga
     3001 atgagacctc ccgcttc
//
LOCUS       DAORHD010000541         3017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37630_length_3017_cov_3.322080, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000541 DAORHD010000000
VERSION     DAORHD010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37630_length_3017_cov_3.322080"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKO06_11755"
     CDS             complement(<1..594)
                     /locus_tag="PKO06_11755"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV70366.1"
                     /translation="MPSQLRLDLGEPDETPSSEPVAEKPARKKRSRKKATAGAEPQER
                     PPEVKSEDVAGGSVTNTNATTQNPSSESNRMTADGSAAVPQGVSPSRAFLYARGAPAQ
                     LPETSAPPADRGVPAPSAVALAEARPLKVAEVTARIKTLLEGDAVLCRVTVEGEISNL
                     NRAGSGHVYFTLKDESSTLRATIWAAQARRIRAAFQNG"
     gene            complement(608..2008)
                     /gene="accC"
                     /locus_tag="PKO06_11760"
     CDS             complement(608..2008)
                     /gene="accC"
                     /locus_tag="PKO06_11760"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015890989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNV70367.1"
                     /translation="MFKKILIANRGEIAVRVMRTCRRLGIRTVAVFSEADREAMHVRM
                     ADEAYCIGPARPDLSYLNIPNILSVAHATKCEGIHPGYGFLSENPNFAEIATKCGFVF
                     IGPSHRAMERMGQKSVARDLMIRSGVPVLPGSQGSIEDPQEGLRLAREIGFPVLVKAS
                     AGGGGRGMRLVETEDQFVSLFNMARAEAQSAFGDGRVYIEKFLRTPRHIEVQIMGDTH
                     GTVLAFGERECSIQRRHQKLIEESPSPAVDETLRQQFFATAVQAAKAIDYVNAGTIEF
                     LLDENGKFFFMEMNTRLQVEHPVTELVCGYDLVEWQLRVASGEPLPMRQDELKPHGWA
                     VECRINAEDVTNGFSPSPGRIETYHVPGGPWVRVDSAAYPGYVVSPNYDSMVAKLIVW
                     APTRLEALDRMRAALTEYRIRGIRTTIPFHTCVMQHPDFRNGEFSTRFVDRNLETILR
                     EIRETESRDTAGVHAS"
     gene            complement(2076..2492)
                     /locus_tag="PKO06_11765"
     CDS             complement(2076..2492)
                     /locus_tag="PKO06_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNV70368.1"
                     /translation="MKTRAIKQLLEAMEQGGYRHLDLQIGDERIKLVRDRDTAPATAA
                     AAEDEALCGKTQTDGTPSGKLVPVLSDRVGVYSVGKTPIQVGGEVKKGQAMGVIKGIS
                     IQDTVAAPLAGIVRQIHVHEGDIVEFGRRLFEIEPA"
     gene            complement(2762..>3017)
                     /locus_tag="PKO06_11770"
     CDS             complement(2762..>3017)
                     /locus_tag="PKO06_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70369.1"
                     /translation="RQLFEHLGTLYERLGDRFDAYEQESIPLDRAFGKYLGRMRSRYD
                     VENAPVWRKVVDYISGMTDLYALQCIRQITLPPPLRFPGV"
BASE COUNT          628 a          914 c          848 g          627 t
ORIGIN      
        1 gccgttctga aaggccgcgc gtatccgccg tgcctgcgcc gcccagatgg tcgcgcgcag
       61 cgtcgacgac tcatccttca acgtgaagta gacatgaccg gacccggcac gattcagatt
      121 cgagatctca ccctcgacgg tcacgcggca gagcacggca tcgccctcaa gcagtgtctt
      181 gatgcgggcg gtgacttccg ccactttcag cggacgcgct tcagccaagg ctacggcaga
      241 cggcgcaggg acgcctctat ctgcgggtgg cgcactggtc tcgggaagtt gcgctggtgc
      301 cccacgtgca tagagaaacg cccgcgatgg tgaaacccct tgcgggactg cagcggaacc
      361 gtcagctgtc atacggttcg actcagacga tggattctga gtggttgcgt tggtattcgt
      421 caccgaaccg ccggccacat cctctgactt cacctcaggc ggacgctcct gcggctcagc
      481 accagcggtc gctttttttc gtgatcgttt tttccgggca ggtttctccg ccaccggttc
      541 tgatgacggt gtttcgtcag gctcgcccag atcgaggcgc agttgtgaag gcacggcggc
      601 ctcccgatta cgaggcgtga accccggcgg tatcacgaga ttccgtttcg cgaatctcgc
      661 gcagaatcgt ctcgaggttc cggtcgacaa agcgggtcga gaactcgcca ttccgaaagt
      721 caggatgctg cataacgcag gtatggaacg ggattgtcgt gcgaataccc cgaatgcggt
      781 actcggtgag agccgctcgc attcgatcga gcgcttcaag gcgcgtcggt gcccagacaa
      841 tcagtttggc taccatcgaa tcatagttcg gactgacgac gtagccagga taagcggccg
      901 aatcaactcg tacccagggg ccacctggca catggtaggt ttcgatacga ccggggctcg
      961 gagagaaacc attggtgaca tcttcggcat tgatgcgaca ctcgacggcc cacccatgcg
     1021 gtttgagttc atcctgtctc atgggcagcg gttcaccaga tgcaacgcgc aattgccact
     1081 cgaccagatc gtacccacag accaactcag tgaccgggtg ttcgacctgc agacgggtgt
     1141 tcatttccat gaagaagaat tttccgtttt catcgagcag aaactcgatg gtaccggcgt
     1201 tcacatagtc aatcgctttg gccgcctgta ccgcggtggc aaaaaactgc tgccgcagag
     1261 tttcatcaac ggccggagag ggcgactctt cgatgagctt ctgatggcgc cgctgaatcg
     1321 agcattctct ctcaccgaac gccagcactg tcccgtgcgt gtctcccatg atctgcactt
     1381 cgatatgacg cggggtacgc agaaattttt cgatgtacac acggccgtcg ccgaacgcgg
     1441 actgcgcctc agcccgggcc atattgaaca gcgatacgaa ctggtcttcg gtttcgacca
     1501 ggcgcatgcc gcggccaccg ccaccggcag aggctttcac cagtaccggg aagccgatct
     1561 cacgggccag tcgcaaccct tcctgtgggt cttcgatact gccctgagaa ccgggcagaa
     1621 ccggtactcc cgaacgaatc atcagatcgc gcgcaaccga cttctgaccc atgcgctcca
     1681 tggctcgatg cgacggacca atgaagacga acccgcattt ggtcgcaatt tcggcgaaat
     1741 tcggattctc ggagagaaat ccgtatcccg gatgaatccc ttcgcatttc gtggcatgag
     1801 ccacggaaag gatgtttgga atattcagat acgaaagatc ggggcgagcc ggcccgatac
     1861 agtaggcttc atcagccatg cgcacatgca tggcttcgcg atcggcttca gaaaaaacgg
     1921 caacggttcg aatgcccaga cggcgacagg tgcgcatgac gcgcacggca atctcgccgc
     1981 gattggcaat gagtatcttt ttgaacatgc tggcctccga aaatctctga tcttggttgt
     2041 gggggcgcgt gatcccaatt ccctgccggc tattgtcagg caggttctat ctcaaacaga
     2101 cgtcttccga actcaacgat atcaccttcg tgtacatgta tctggcgtac aataccggca
     2161 agcggagcgg caacggtgtc ttgaatgctg atgcccttga tgacacccat agcctgacct
     2221 ttcttgactt caccgccgac ctgtatcggg gttttgccaa ccgagtacac accgacgcga
     2281 tccgacagta ccggcaccag ttttccggag ggcgtgccgt cggtctgagt cttgccgcac
     2341 agcgcttcat cttcagcagc ggcagctgtc gcgggggccg tgtcgcggtc acgcaccagt
     2401 ttgatgcgct cgtcaccgat ctgcagatcg agatgccgat agccaccctg ttccatcgct
     2461 tcgagcagct gcttgatcgc ccgggttttc atgtctttct cccaactgcg gtttccggta
     2521 catcgaccgg gaagacgggc ggtatgctag cacacccccg caaccgaccg caagtcttgt
     2581 gccctgccgc cccgcagcca tctacccgga acagtggcgc gtgccctcga ccgccgttcg
     2641 cacagctgcc atgaatcagg ctcttcaccc ggagggcaac cgtgactcaa ctctgttgaa
     2701 tcgagtgtag gaatcgccgc acgggtcgtt cagatacggg cacgcccagg acgtcgcggg
     2761 ctcagactcc gggaaaacgc aggggtggtg gcagagtgat ctggcgaata cactgtaaag
     2821 cgtacaagtc cgtcatacca gatatgtagt ctacaacctt tcgccaaacc ggagcattct
     2881 cgacatcata tctcgaacgc atgcgtccga gatacttccc gaaggcgcga tcaagcggta
     2941 tcgattcttg ttcgtaggca tcgaaccgat cccccagccg ctcatacagg gtgccaagat
     3001 gttcgaacag ctggcga
//
LOCUS       DAORHD010000542         3009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37780_length_3009_cov_2.964116, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000542 DAORHD010000000
VERSION     DAORHD010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37780_length_3009_cov_2.964116"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKO06_11775"
     CDS             complement(<1..799)
                     /locus_tag="PKO06_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70370.1"
                     /translation="MRVPSREPSSLVQAGLFVTLLVPAILAIYLFEALLGSYAELTLQ
                     QYRDLLPRAAKVIEENTNPANLLWSRFSKALGENRAEITRENAQAMADRARRALQVPA
                     NVYCFNSSGSVVFPLEQTSDVQEMETFYRGYQNFRLRRPLTPENLRLDRAAKQVFRKR
                     FGRGNFLKLVDAMVCNWKSEIPGSPPRYIGYLPIVRQNDATLYSGIFIEVDDTHIPRS
                     HLFEHGLRACNEQIGYTAFLASDGASIVAERFSAWFPRERLTEIPIST"
     gene            1049..1594
                     /locus_tag="PKO06_11780"
     CDS             1049..1594
                     /locus_tag="PKO06_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70371.1"
                     /translation="MVRTVLIIGVLGCFLCVVLFVTNQDAIRRYIRSHVKVAPQKNSL
                     DLDLKSLDLAHVQGTMNLSVFNGLPVGATLVKLQYSVFMNGAEICHGLQAHPQAPIAP
                     TATSTLKISFSVDSQKVRAAIGRTTPEQAIALGLNLIDRMKGKKPLTNSLKGLIRISG
                     NAEIQVFTSSVDIPVEFSRNL"
     gene            complement(1598..2422)
                     /locus_tag="PKO06_11785"
     CDS             complement(1598..2422)
                     /locus_tag="PKO06_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70372.1"
                     /translation="MRGHWWLVALLMVSSSALGVQAAPIDDFLAFNERFYYLDRQSVG
                     TISARLECPALINSMKTLREQLAHMQKAVSITGEPASYTLTYHPQHGLKLNPPELKVE
                     QIASEGVKNQETFARGLEQVRGGIQQQLEGIVQTITSIFEGMTAPVAKDYQVESIVQN
                     GEVWTMKYTDNEGPVVATFSGTMFEKNQMTPGDTIRSREEYKSNDAGKLLLSRAQVTI
                     ENAMACIELEMTMTYEQVGGIQFPVGIVSAFAQKIQTMEQKGTVEIFLKDLKLATD"
     gene            complement(2615..>3009)
                     /locus_tag="PKO06_11790"
     CDS             complement(2615..>3009)
                     /locus_tag="PKO06_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70373.1"
                     /translation="GSNVGFWLSGHLEGAGVAVASYQSVCASFTWLTKLSASLTEIEM
                     QEDVRIRFSNPQRVVPVVWSRKAAMNHTLNWTVPFLILLLFINFLLEGSRKGILICLA
                     VAALFGALTYADLPVTDHIDRVSLKFNL"
BASE COUNT          695 a          849 c          814 g          651 t
ORIGIN      
        1 ccgtgctgat cggtatctcc gtcaggcgtt cccgggggaa ccaggcactg aaacgttccg
       61 caacgattga agctccatcc gatgccagga aagccgtgta tccgatctgc tcattgcagg
      121 cccgcaggcc gtgttcgaac agatgagatc ggggaatgtg cgtgtcgtcg acttcgatga
      181 agatgcccga atacagcgtg gcatcgttct ggcgaacaat cggtagatat ccgatgtagc
      241 gcggcggaga tccggggatc tcggatttcc agttacaaac catggcatcg acaagcttga
      301 gaaaattgcc acgaccgaac cgttttcgga acacctgctt ggccgctctg tctaagcgca
      361 gattttcagg ggtaagcggc cggcgaagac gaaagttctg atatccccgg tagaaagtct
      421 ccatttcctg aacgtcagaa gtctgctcaa ggggaaacac caccgaaccg gaactgttga
      481 aacaatacac gttggcgggc acctgaagag ctcttcgggc tcgatccgcc atggcctggg
      541 cgttctcacg cgtgatttcc gcgcggttct cgccgagtgc cttcgaaaac ctgctccaga
      601 gaagattcgc cggattggtg ttttcctcga tcaccttcgc cgcccgggga agaaggtcgc
      661 gatactgctg gagagtcagt tcagcgtagc tgccgagcaa agcctcgaag agatagatgg
      721 cgagaatggc cggcaccagc aaggtgacga acaagccggc ctggaccaaa ctcgaaggct
      781 cgcgggaagg aaccctcatg gccggcattt cttatgggga aacagcttat cagacaagca
      841 tctacgccct ttcccggaaa cggtaccaca cacccccctt tcgaacaagg gacctgcatg
      901 ggaagacggc gcgcgaggtc aacctggcga gcccggtgag tggtatcatg ccttgcgccc
      961 acacagtttt cgaactgaaa gtgcaagaga agccacttca tcgtcgaggt aaatcatgat
     1021 gagatattcc aagcccacag caggcggaat ggtgagaacc gttctgatca ttggcgttct
     1081 gggctgcttc ctgtgcgtgg ttctgttcgt cacgaaccag gacgccatca ggcgctacat
     1141 tcgctcgcac gtgaaagtgg caccgcagaa aaactcgctc gacctggacc tgaaatctct
     1201 cgacctggca cacgtgcagg gaaccatgaa cctgagtgtc ttcaacggtc tgccggtggg
     1261 agccactctt gtgaagctgc agtattcggt gttcatgaac ggcgcggaga tctgtcacgg
     1321 actgcaggca cacccgcaag ccccgatcgc gccaactgcc acgtcgacgc tgaagatcag
     1381 tttttctgtc gatagccaaa aagtccgagc tgcaatcgga cgaacaacgc ctgaacaagc
     1441 gatcgcactc ggattgaatc tgatcgatcg aatgaaaggc aagaaaccgc tgacgaactc
     1501 cctcaagggc ttgatccgca tctccggcaa cgcagagatt caggtgttca ccagttccgt
     1561 cgacattccg gtcgaattct cgcgaaatct gtaaacttca gtctgtggcc agtttcaggt
     1621 ctttcaggaa gatctcaacg gtgcctttct gttccatcgt ctggatcttc tgagcaaagg
     1681 cactgacgat gccaaccggg aactggattc cgccgacctg ttcataggtc atggtcatct
     1741 cgagttcgat gcaggccatc gcattttcaa tcgtcacctg agctcggctc aacaaaagtt
     1801 ttcctgcgtc attcgacttg tactcttcac gactgcgtat cgtatctccg ggcgtcatct
     1861 ggtttttttc gaacatggtg cccgaaaacg tcgcaacgac cggaccctcg ttgtcggtgt
     1921 atttcatagt ccagacctcg ccattctgga caatcgactc aacctggtag tctttggcga
     1981 ccggagcggt cattccttca aaaatgctgg tgatcgtctg aacgattcct tcgagctgct
     2041 gctgaatgcc gcctcgcacc tgttcaaggc cacgcgcgaa cgtttcctga ttcttcaccc
     2101 cttcagacgc gatctgttcg actttcaact caggcgggtt cagcttcagc ccatgctgcg
     2161 ggtgataggt cagcgtgtat gatgctggtt cgccggtgat cgagacggct ttctgcatgt
     2221 gcgccagctg ttcgcgcagg gtcttcatgc tattgatcaa agccgggcac tcgagccgag
     2281 ccgagatagt accgaccgac tgccggtcga gatagtagaa ccgctcgttg aaggccagaa
     2341 agtcatcgat gggcgctgcc tgcacgccaa gcgctgagct gctcaccatc aacagtgcta
     2401 ccaaccacca atgtccacgc atgtgttttc ccttccttca gacagagata acgaacgttt
     2461 ttccagacac cgtcatgata cgcgaatcac cacccgaatc acatgttctt gtcacaaaca
     2521 gttaacaggt gcttgcgtta tcgaacgtcc ataacgaatc gccatactgg ctcgggacat
     2581 gggttgccgc ttccccccac ccactgcaca atcgtcacag attgaacttt agcgagacgc
     2641 gatcgatgtg atcggtgacc ggcagatcag cgtaggtcag ggctccgaaa agcgccgcga
     2701 ccgcgagaca gatcagaatt cccttccggc ttccttcgag caggaagttg atgaacagca
     2761 gtaggatcag gaacgggacc gtccagttga gagtgtgatt catagcggct tttcgagacc
     2821 acaccacggg aacgacacgc tgtgggttgg aaaagcgaat gcgaacgtct tcctgcatct
     2881 cgatctcggt cagtgatgcg gagagtttcg tgagccaggt gaagctggca cagacgctct
     2941 gataggatgc gacagcgacc cccgcgcctt cgagatgccc tgaaagccag aaaccgacat
     3001 ttgagccgg
//
LOCUS       DAORHD010000543         3008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37789_length_3008_cov_4.270234, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000543 DAORHD010000000
VERSION     DAORHD010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37789_length_3008_cov_4.270234"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKO06_11795"
     CDS             <1..381
                     /locus_tag="PKO06_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70374.1"
                     /translation="VSPPYENTKIIGSNTVSVNLVLNRMEITKSFSGIPLRLKNSPEY
                     HCTVTPSSYTMVLEGYAEKLKGIVPSDLEVILDVRDKKPGVIEVPLNCPIGLPEGVKI
                     LDMLPAKVQIEISEPTSDSGSISP"
     gene            464..2794
                     /locus_tag="PKO06_11800"
     CDS             464..2794
                     /locus_tag="PKO06_11800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70375.1"
                     /translation="MEPHIQKLIEQFQLADKDRKLRLLTEISATKDEGVLQFLVTCLG
                     DETWLVRKSAADLIFEYQEAALPALSAALNSYSEDIQHWSLQVLTRLGKKGAPSILRG
                     LKSPNHDVRYFACMALGDLREPQGVTPLIRALGDETWHVRKSASDALVKYGEEVITAI
                     EAVMNKTTDEDVRFWAIKSLGKLGPKAQRILLEALRTGDKHLRYVIAAALGESGDRRV
                     IKVLIDSLGDPDWTIRKSATQALAEIGENAVEMLIEGLKEPNEDVRDGCIIAIFRIGD
                     VAIVKFFSFVESVDDNTRYLLRKSLVKLGTRVVEPLIRLFKSTKPEVMVFCAATLGEI
                     GTPKAVPVLIEGLSNKDWNVRRSCAYALTEIGEKGVDRIADALASANEDTRYWVTRIL
                     ESIGEPGMPYLVKALSDKNKNIRYFAARALGSSSNPDVMRDLIRSLADTSWSVRKAAA
                     ESIIKLEAVNVDHLLRNISNDNSDIRFWISQIIRQTGVDLLEQIHERVRVGDPEMRLC
                     ACQALSWIADPRSTDVLVDALRDGNEWSRIYAAIALGRIGDPRAIVPLLRSMSDRNTE
                     VRRNILQAFEKLGPKVLETIAKSVESSDIGLRRNSAIALGEMRATQGLDHLILLAEDP
                     EDKVRQSAIEALGHFSSTRVTPILLKALGDTSFHVRQAAGGGLAEQAFPESIPELITL
                     IQKTKDERDQRLLRRTMAQIAAKIPQSFIALFVHESVNIRTIAAEALIGAGLPVLQIL
                     TEAESAAHERKDETTAFWCQKVYRKIKNPQEPLPNA"
     gene            2787..>3008
                     /locus_tag="PKO06_11805"
     CDS             2787..>3008
                     /locus_tag="PKO06_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70376.1"
                     /translation="MPEAGRPTSPGPRTPDPVRAAELRPWLILAAMHWLGATVRAGMI
                     TELGSADAALQASPQMLAAIPGIDQRRLDR"
BASE COUNT          658 a          902 c          860 g          588 t
ORIGIN      
        1 gtcagtcctc cctatgagaa cacgaaaatc atcggctcga atactgtttc cgtcaacctg
       61 gttctcaacc gcatggagat caccaagtcg ttttccggaa tccccctgcg tttgaaaaac
      121 tcgcctgaat accactgcac ggtcactccg tcgagttaca cgatggttct cgaagggtat
      181 gccgaaaaac tgaagggcat cgtcccctcc gacctcgaag tcattctcga cgtgcgcgac
      241 aagaaaccgg gtgtgatcga agttcccctg aactgcccca tcggccttcc cgaaggggtc
      301 aaaattctcg acatgcttcc cgccaaggtg cagatcgaga tcagcgaacc cacatcggat
      361 agtggaagta tcagcccatg aaatgggtat atcgtcctgc cggaggccgc gcatgatgaa
      421 gcatgaacgg ctcgggcaac cgcggagaca gtgaggcacg ctcatggaac cacacataca
      481 aaagctcatc gaacagtttc aactcgcgga taaagaccgc aaacttcgcc tgctaaccga
      541 gatctcggcg accaaggacg agggggttct gcagtttctc gtcacctgtc tcggcgacga
      601 gacctggctg gtacgcaagt cggccgccga tctgattttc gaatatcagg aagccgctct
      661 cccagctctt tccgcggccc tcaactcgta cagtgaagac attcagcact ggtcgctgca
      721 ggtgctgacc cgactgggca agaagggcgc tccttcgatt ttgcgcgggc tcaagagccc
      781 gaatcatgac gtgcgctact tcgcctgcat ggccctgggt gatctgcgcg aaccgcaggg
      841 agtcacgcca ctgatccgcg ccctgggcga tgaaacctgg catgtccgaa agtccgcgtc
      901 agatgctctg gtcaagtatg gagaagaagt catcacagcc atcgaggccg tgatgaacaa
      961 gaccacggac gaggatgtgc gcttctgggc gatcaaatct ctcggcaagc tcggtcccaa
     1021 agcgcaacgc attcttctgg aagccctgcg caccggcgac aagcatctgc gctacgtcat
     1081 tgccgccgcg ctcggcgaat cgggtgatcg gcgcgtcatc aaggttctga ttgattctct
     1141 cggagacccg gactggacga tccgcaagag tgcgacgcag gcgttggcgg agatcggtga
     1201 gaatgccgtt gaaatgttga ttgaggggct gaaagaaccc aatgaggacg tgcgcgacgg
     1261 ctgcatcatc gccatcttcc gtatcggcga tgtcgccatc gtcaaattct tttccttcgt
     1321 cgagagcgtc gatgacaaca cgcgttacct gttgcgcaag agtctggtca aactgggaac
     1381 acgcgttgtc gagcctctga taagactttt caagagcacc aaacctgagg tcatggtgtt
     1441 ctgcgccgcc acgctcggcg agatcggcac cccgaaagct gtgccggtgc tcatcgaagg
     1501 tctttccaac aaggactgga acgttcgccg aagttgtgcc tacgccctga ccgaaatcgg
     1561 agaaaagggc gttgaccgca tcgccgatgc ccttgccagc gcaaacgaag acacgcgcta
     1621 ctgggtgacg cgcattctcg agtcgattgg tgagcctgga atgccatatc tggtaaaagc
     1681 tctctcggac aaaaacaaga atatccgcta ttttgcggcc cgtgcgctgg gcagttcatc
     1741 aaatccagat gtcatgcgcg atctgattcg atcgctggcc gatacctcct ggagcgtgcg
     1801 caaggccgcg gccgaaagca tcatcaagct ggaagccgtc aatgtcgacc acctgctgcg
     1861 caacatcagc aatgacaaca gtgacatccg tttctggatc agtcagatta tccgacaaac
     1921 cggcgttgat ctgctcgagc agattcatga acgggtacgg gtgggcgacc ccgaaatgcg
     1981 tttgtgcgcg tgccaggccc tgagctggat cgccgacccc cgatcgacgg acgttctggt
     2041 tgatgctctg cgcgatggta acgagtggag ccgcatctac gccgccatcg cgctcggacg
     2101 catcggtgac ccgcgcgcca tcgttcctct gctgcgcagc atgtccgacc ggaacaccga
     2161 agtgcggcgg aatatcctgc aggctttcga gaaactgggg cccaaggttc tcgaaaccat
     2221 tgccaagagc gtcgaaagct ctgatatcgg tctgcgtcgg aacagcgcca tcgctcttgg
     2281 ggagatgcgc gcgacgcagg gtctcgacca cctgattctg ctcgccgaag accccgagga
     2341 caaggtgcgc cagtctgcca tcgaagccct gggccatttc tcgagtacac gggtcacgcc
     2401 gattctgctc aaggcgctcg gcgatacgag ttttcatgtg cgacaggcgg cgggcggcgg
     2461 gttggccgag caggctttcc ccgagtcgat ccccgaactg atcacgctga tccagaaaac
     2521 caaagacgag cgtgaccaac gcttgttgcg ccgaaccatg gcccagatcg ccgccaagat
     2581 tcctcagtca ttcatcgctc ttttcgtgca cgagagtgta aacattcgca ccatcgctgc
     2641 tgaagcgctt atcggcgcag gactaccggt actacagatc ctgaccgaag ccgaatccgc
     2701 tgctcacgag cgcaaggacg agacgaccgc cttctggtgt cagaaggtgt atcgcaagat
     2761 caagaatcca caggaaccgc tgccgaatgc ctgaggccgg cagaccgaca tcgccggggc
     2821 cccggactcc tgatccggtt cgcgcggcgg aactgcgccc gtggctgatt ctggcggcca
     2881 tgcactggct gggcgccacg gttcgtgccg gcatgatcac ggaactcggt tcggccgatg
     2941 cagctttgca ggcttccccg cagatgctgg ccgccattcc cggcatcgat cagcgacgtc
     3001 tggatcgc
//
LOCUS       DAORHD010000544         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37850_length_3005_cov_4.076949, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000544 DAORHD010000000
VERSION     DAORHD010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37850_length_3005_cov_4.076949"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKO06_11810"
     CDS             <1..352
                     /locus_tag="PKO06_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70377.1"
                     /translation="TGEINVEKPGELFGFAANQQPFNGSPCPWCGSSRTWRYHHWNDN
                     YIRGNGVEVDKSDYYEVCSHCGVLFVTHSGYSDKERNGVFPMRGSARTLTGKLRDALE
                     NFLKTGEVSLDARE"
     gene            complement(425..>3005)
                     /locus_tag="PKO06_11815"
     CDS             complement(425..>3005)
                     /locus_tag="PKO06_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70378.1"
                     /translation="QTPRTIDMKTEDIDFSRFVPPLKILRDRLLLAGGLTTDQVYART
                     WHNWSVMMSQAMVMFGRECHEVLTLERFMTPSIFKNIADYRDLLTGLPNYIRTAEASL
                     GLIGKILPLTIREVGDNFEVWNWAIGAQRTLGHYHQLAERGLVGRHILARLEGVAAPG
                     EQVGFNGQRLTQAFSAADSTEVLTNTQLAGKTFGFAMAIFSIYMMAQDYQELRGTWNY
                     NSSLENFLGVAGLVLDAAGLLAPLLFPPPYGEIIFTLQMLLLVGKGVVSAYKEWMDTQ
                     EMAYQDSHRFLYDADPEFKSFVDNESQWTDQIKSEGMRILQETNRTYRTEPSAFTSGE
                     AVPMQHGLEAAMRQAQVMTYYNRSHPTPPMDEDGLTSMKRMWEAKMSYMQWRPTQRDV
                     DFDADLSRQGFWDRVGTRFNNFWYVVSRDTRERFAPIGRWWNSDNIDANKPRVWYNPD
                     FFLYSKIKRYVDAANPAMVRYLKYRMETAPYHLMPLVEIPVDEWGPIMGDGPAADALK
                     CDVYMCAMKNLRSLDSLIKGMTQEIKNLTDTTKDNLENVRNNTIDRWARPALTALVDL
                     AKAYKEDKNRALDSEIWDKLESLLDFDAPPGEGEKTPDSVVRANRLLIQNHLNMFPYM
                     LGKLEAEAVAAIDGTLNYFLMAKILEDHLGPKVKQETDQLLASIRHTKFRDFLEHKRY
                     LHFGDGWFSEPNIPKKVMEDTVKSLTEADGATITAFKGALQNLIDADDYDWHHRVSDE
                     RVMARYRLGIRFCLCRAIDAIKRWKEIQTETNVRIKIPHNANWHYHANDWTSQWFDAN
                     DDETRAIFIEEPFCSALFNYPGGPTIADKAGEAADMRALHSDLSHYRRPAKLFERLPA
                     QAP"
BASE COUNT          619 a          811 c          903 g          672 t
ORIGIN      
        1 cacaggggaa atcaacgtgg aaaaacccgg agaattgttc ggcttcgcgg cgaatcagca
       61 gccattcaac ggctcaccgt gtccgtggtg cggttcgagc cgtacgtggc gttaccatca
      121 ctggaatgac aactacattc gtggcaacgg tgtggaagtc gacaagagcg actactatga
      181 agtgtgctcc cattgcggtg tactcttcgt cacccacagt ggttactcgg acaaagagcg
      241 aaacggggtt ttccccatgc gtggctcggc caggacgctg accggaaaac tgcgtgatgc
      301 gctcgagaac tttctgaaga ccggcgaagt cagtctcgac gcccgcgagt gagagtctgc
      361 aagaaagaaa aagccacgcg acgcttcagg gcgcagcatc gcgtggcctt gggccataga
      421 cccattatgg agcctgggcc gggagacgtt caaagagctt cgcggggcgg cgatagtggc
      481 tcagatcgga atgcagcgcg cgcatgtcgg cggcttctcc ggctttgtcg gcaatggtgg
      541 ggccgccggg gtagttgaac agagctgaac agaacggctc ttcgatgaag atagcgcggg
      601 tctcatcatc gttggcgtcg aaccactggc tggtccagtc attggcgtga tagtgccagt
      661 tggcattatg gggaatcttg atgcgaacgt tggtttccgt ctgaatctct ttccaacgct
      721 tgatggcatc gattgcccgg cacagacaga agcgaatgcc aaggcggtat cgggccatga
      781 cccgttcatc gctgacccgg tgatgccagt cgtaatcatc agcatcgatc agattctgca
      841 gcgctccctt gaacgcggtg atcgtggcgc cgtcagcttc ggtcaaactc ttgaccgtgt
      901 cttccatgac ctttttcgga atgttcggtt cagaaaacca gccgtcgccg aagtgcagat
      961 accgcttgtg ttccagaaag tcacgaaact tggtgtggcg aatgctcgcc agcagttggt
     1021 cggtctcctg cttcactttc ggcccgagat gatcttcgag aatcttggcc atcaggaagt
     1081 aattgagtgt gccgtcaatc gctgccacag cctcagcttc gagtttgcca agcatgtacg
     1141 gaaacatgtt cagatgattc tgaatcagca ggcgattcgc ccgaacgacg ctgtcggggg
     1201 tcttctcgcc ctcgcccggg ggcgcatcga aatcgagcag cgattcgagt ttgtcccaga
     1261 tctctgagtc gagggcccgg ttcttgtctt ctttatacgc cttggcaaga tcaaccagag
     1321 ccgtgagggc ggggcgagcc cagcgatcga tcgtgttgtt gcggacattc tcgagattgt
     1381 ccttggtcgt gtccgtcaga ttcttgatct cctgcgtcat gcctttgatc aggctgtcga
     1441 gtgaacgcag gttcttcatg gcgcacatgt agacatcgca tttcagcgca tcggccgcgg
     1501 ggccgtcacc catgatcggt ccccattcgt cgaccggaat ttcaaccagc ggcatgagat
     1561 gatatggcgc ggtttccatg cgatacttga gataacgcac catggcaggg ttcgccgcat
     1621 cgacatagcg cttgattttc gagtagagaa agaaatccgg attgtaccag acgcgtggtt
     1681 tgttggcgtc gatgttgtca ctgttccacc atcgtccgat cggagcaaag cgctcgcgtg
     1741 tatctcggct gaccacatac cagaaattgt tgaaacgcgt gccgacccga tcccagaaac
     1801 cctggcggga aagatcggcg tcgaagtcga catcgcgctg agtgggtcgc cattgcatgt
     1861 agctcatctt cgcttcccac atacgcttca tgctggtcaa accgtcctcg tccatcggcg
     1921 gagtcggatg actgcgatta taataggtca tgacctgggc ttgacgcatg gcggcctcga
     1981 gaccgtgctg catcgggacc gcctcgccgc tggtgaaggc ggaaggttcg gtgcgatagg
     2041 tgcgattcgt ttcctgtaga atgcgcattc cttccgactt tatctggtcg gtccactggg
     2101 actcattgtc gacgaaagat ttgaactcag gatcggcatc gtagagaaag cgatgtgagt
     2161 cctgataggc catttcctgc gtatccatcc attctttgta ggctgaaacc acgcccttgc
     2221 cgaccagaag cagcatctgc agcgtgaaga tgatctcgcc gtaggggggc ggaaacagca
     2281 gcggtgccag gagtccggcg gcatcgagta ccaatccggc gactccaaga aagttttcga
     2341 gagagctgtt gtagttccag gttccgcgca gttcctgata gtcctgggcc atcatgtaga
     2401 tcgaaaagat cgccatggcg aatccgaacg tctttccggc cagctgggtg ttggtgagca
     2461 cctcggtcga atcagcggcc gaaaatgcct gggtcagacg ttgtccgttg aagcccacct
     2521 gctcgccggg agcggcgacg ccttcgagcc gggcgagaat gtgccgtccg accaggccgc
     2581 gttcagccag ctggtgataa tgtccaaggg ttctctgagc gccgatggcc cagttccaga
     2641 cctcgaaatt gtctcccact tcgcgaatcg tcaggggcag aattttgccg atgaggccga
     2701 gcgatgcttc ggccgtgcga atgtagttgg gcaggccggt cagcagatcg cggtagtcag
     2761 cgatgttttt gaagatactc ggggtcatga agcgttcgag tgtgagcact tcgtggcact
     2821 cgcggccgaa catgaccatc gcctgcgaca tcatcaccga ccagttgtgc caggtgcgtg
     2881 catagacctg atcggtcgtc aggccaccgg cgagcaacag acggtcgcga agaatcttca
     2941 gcggcggcac gaaccgcgag aagtcgatgt cttcggtctt catgtcgatg gtgcgcggcg
     3001 tctgg
//
LOCUS       DAORHD010000545         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37892_length_3003_cov_3.133650, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000545 DAORHD010000000
VERSION     DAORHD010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37892_length_3003_cov_3.133650"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKO06_11820"
     CDS             complement(<1..323)
                     /locus_tag="PKO06_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70379.1"
                     /translation="MRIRLISLFLVALLALCSTAQADLYLSSGKYQFNVRVENSHFKD
                     VRDARGMVFVNGNTARISVDAPGYRSGYEYVYLNANQTYYYTRVRLDEPMCSATVIDT
                     TGKLVS"
     gene            619..>3003
                     /locus_tag="PKO06_11825"
     CDS             619..>3003
                     /locus_tag="PKO06_11825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70380.1"
                     /translation="MDTRELFFRAEELFSEARWQELADMLHAEEGGEEARAVIFFWRG
                     RLARAKGDADAAVQALEQVVSLEPQTPLFLMHLAQALEMQQAMSFLATLQEDQTQLTK
                     IESTWRQVLELAPESGLYRASYAEFLVGRQRFDEALALICEARTLCPAHEEIRRLWLR
                     FNLKMGGHFATTAGCNLEAGLRCLLAVLEEIPDLHDALLFAGICCRKLGRREEAISFY
                     QRCCALAREGRAEPDPLPFNDLAELLLASGDLAGAERVVEEGLRLDPESPALRGLRDD
                     LRQRRPLVAPPPGETAAAEILVDTKVSESGAELTSTPSDASSIELQPIAESVRKGMRD
                     VLSGQDDRKWVGGLSELFPPGASPLAYWHAALEESFSNDDDRNWSRYLAVLEVSLSRW
                     QAAVEMSPALAERLKLWCDRLTSLSRRRIDPESGKLLPPDSAYAQGAGIWRELFSTSN
                     FCTRAADTSFGRWLIDRADFWKEELVYYLLHQRPRAFLALLRLMLVLAARLPGFAERC
                     VWLPVVLSSYLNRYSVEGASESRDSSQTPATTRQELALLINGWLETPEPAAPGWTALV
                     ASVRQAVSRTRPADVPETVALGDEELTVEAVFRFALEEVLADELMTREETEILRTLRD
                     YLEIPDATYQQLFREVSERASRRQLTRLNRDFSASEFLYRLLLKTMEDGRVTPAEKML
                     IKRAAAALLIDQQTFNEQYWRAEAEYRRRLTALRAASTGQLEEDPVLRRLRKAEKLNE
                     RLAELLKHERTAELRTIIQNACSDVASRAANQKSSSTRTSVSPVVAFLSWEPAVF"
BASE COUNT          593 a          833 c          973 g          604 t
ORIGIN      
        1 gacacgagct tgccggtggt gtcgatgacc gtcgccgagc acatcggttc atcgagtcgg
       61 acgcgcgtgt agtagtacgt ctggttcgcg ttcagataca catactcata accgctgcga
      121 tatccggggg cgtcgacact gatgcgcgcg gtgttgccgt tgacgaacac catgccgcgg
      181 gcgtcgcgaa catctttgaa atggctgttt tcgacccgga cattgaactg gtacttgccg
      241 gatgacagat acagatcggc ctgagccgtg ctgcacaagg caagcagggc aaccagaaac
      301 aggctgatga gacgaattcg catggatggc ctccgttcac gacaataaat ctgggggatt
      361 ttccctctct tctctaaact atcgctgaaa cgcgcaaaaa cacaaggagc acatctgcaa
      421 aacccgtggc gagccgactt tggccgctca cggtcacaca aaatatgtgg caggaaagca
      481 cgtggtacga tagccacagg gctcagaccc ttctattcgg aacgttctga tcatgccccc
      541 gatgtgtgca gttggtgaaa ggaggaacgc ctgaggtaga atgacatgat tgaaccgact
      601 gggccgaggg aggaacgagt ggacacacgt gagttgtttt ttcgcgctga agagctattt
      661 tcagaagcgc gctggcagga actagcggac atgctgcacg cggaagaagg cggcgaagaa
      721 gcgcgagcgg tgattttttt ctggcgtggt cgcctggcgc gggcgaaggg cgatgccgat
      781 gcggccgtgc aggctctgga gcaggtggtg tcgcttgaac cgcagacccc gctgtttctg
      841 atgcatctgg cgcaggcact cgaaatgcag caggccatga gttttctggc aacgttgcag
      901 gaagaccaga cgcagctgac caagatcgag tcaacgtggc gtcaggttct ggagctggct
      961 cccgagagcg ggctgtatcg cgcgtcatac gcagagtttc tggtcggccg ccagcgattt
     1021 gacgaggcgc tggccctgat ctgcgaagcg agaacgctgt gtccggctca tgaagaaatt
     1081 cgccgtctgt ggctgcgctt caacctgaag atggggggac actttgccac gactgccggg
     1141 tgcaatctgg aagcaggtct tcgctgtctg ctggcggtac tcgaagagat ccccgacctg
     1201 cacgatgccc tgctgtttgc cggcatctgc tgtcgcaagc tcggccggcg tgaggaggcg
     1261 atctccttct atcagcgttg ctgtgccctg gcgcgcgagg gtcgggccga gcccgatccg
     1321 ctgccgttca atgatctggc ggaactactg ctcgcatcgg gagatctcgc cggggcggaa
     1381 cgggtcgtgg aagaggggct ccgtctcgac ccggaaagtc cggcgctgcg tggcttgaga
     1441 gacgatctga gacagcgtcg accgctggta gccccgccgc cgggtgaaac cgcggcagcg
     1501 gagattcttg ttgacacgaa ggtctctgaa tccggggccg aattgacttc gacaccctct
     1561 gatgcctctt caattgagct gcagccgatt gcagagtcgg tacgcaaagg catgcgcgat
     1621 gtactgtcgg ggcaggacga caggaagtgg gtcgggggtc tgagcgagct gttccccccg
     1681 ggtgcctctc cgctcgccta ctggcatgca gccttggagg agagcttttc caacgatgac
     1741 gatcgaaact ggagtcgcta tctcgcggtg ttggaagtga gtctgtcacg gtggcaagcc
     1801 gcggtcgaaa tgtcaccggc tcttgccgag cggctcaagc tctggtgtga tcgcctgacc
     1861 agtctcagtc ggcgccgcat cgaccccgaa agcggaaagc tattaccgcc tgattcggcg
     1921 tatgcgcagg gagcgggtat ctggcgtgag ctgttcagta cttcaaactt ctgcacgcga
     1981 gctgccgata cgagcttcgg gcgctggttg atcgatcgtg ccgatttctg gaaggaagag
     2041 ctggtctact atctgctgca tcagcggcct cgggcctttc ttgccttgct gcgactgatg
     2101 ctggtgctcg ccgcgcggct tcccggtttt gcggagcgct gtgtttggct tccggtggtt
     2161 ctttcttcat atctcaaccg gtactctgtc gaaggggcga gtgagagccg tgattcgagt
     2221 cagacgcctg cgacaacccg ccaggagctg gctctgttga tcaacggctg gctcgagacc
     2281 ccggagcccg cggccccggg gtggacggcg ctcgtcgcct cggtccgaca ggcggtttcc
     2341 cgaacccgac cggcagatgt gccggaaacg gtcgctctgg gtgacgagga gctgaccgtc
     2401 gaagcggtgt tccgattcgc gctcgaggaa gtgctggcgg acgaactcat gacccgcgaa
     2461 gagacggaga tcctccgaac gttgcgcgac tatctcgaaa taccggacgc aacctaccag
     2521 cagttgtttc gtgaggtcag cgaaagggcg tcacggcgac agcttacgcg tctcaatcga
     2581 gacttttccg cgagtgagtt tttgtaccgg ttgcttctca agaccatgga ggatgggcgc
     2641 gtcacaccgg cagagaaaat gctcatcaag cgggcggcgg cagcgctgct gatcgatcag
     2701 cagaccttca acgaacagta ctggcgtgcc gaggccgagt atcgacgtcg attgaccgcg
     2761 ttacgggcgg cgtcaaccgg gcagctcgag gaagaccccg tcctgcgtcg actgcgaaaa
     2821 gccgagaaac tcaacgagcg acttgctgaa ctgctcaagc atgaacgaac ggccgaactg
     2881 cgtacgatca tccagaacgc gtgcagtgat gtggccagtc gagcggcaaa ccagaagtcg
     2941 tcgtcgaccc ggacgagcgt atcgcccgtg gtggcgttcc tgtcctggga gccggcggtt
     3001 ttc
//
LOCUS       DAORHD010000546         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37906_length_3002_cov_4.295894, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000546 DAORHD010000000
VERSION     DAORHD010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37906_length_3002_cov_4.295894"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKO06_11830"
     CDS             <1..552
                     /locus_tag="PKO06_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70381.1"
                     /translation="PPTKNADPLKREREIAVIKRISEYLAEERYRFAHIGDDCIELYL
                     QGKHLTMRMIIYAHNGHLVVRVPEFIRNQNLLFPAFLRTILTVQGEYFDIRFELADDG
                     KSLSACANHILEDNEMTRTQFNQCMTVVAFIVDQHYPLFLKVLYKEHLESGEPMTEDE
                     KKAESGESDSTPTTDPPDGHSLN"
     gene            581..1705
                     /locus_tag="PKO06_11835"
     CDS             581..1705
                     /locus_tag="PKO06_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70382.1"
                     /translation="MNATTFAQVRQTAVTIACIICLLFAVSSAWARTIQIPAWYEFSA
                     ALEEVPQIDQPVHFIASLTALVDDIDNVSVSLVLPPGWSADKTSDTLAKLPAKQTHGF
                     RFLIKPAGPLPNGGIGCSFQARVPKAALIESVRATGHDLGEAMAQAIANSPDVGNGYT
                     DVAFALFAEEGFFPLGETMWLAYDDRFKPSEALRGPSVWKDELISTHQAETDVEMFDR
                     LKQQLTSNPRLAEQLARNGIDVNRKRGDALHAQLVLATEAYLKGEFPRCLEWLTRFEG
                     EMAAGEAGQQLELRIAGRNLRSLCLWASGNKRDAEELLRETFYLNRKLPVQRYVLRNL
                     GLLTLEKGDRNTAREMFRLALEIKPAYSLLKKEFALLQKK"
     gene            1756..>3002
                     /locus_tag="PKO06_11840"
     CDS             1756..>3002
                     /locus_tag="PKO06_11840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70383.1"
                     /translation="MDAVQTLFADLRSPDLSIRFSVLSRIEGLEWTPEQIQTLRELFS
                     AETDPGTRFHLQKILTRVSSGLEKKANAEVAVELTSLLRKPERDDLGLAILLEQVSRK
                     EAPLVATKLRHADWTSFSAEVLPFVISFFRRHGSPEDVPTLEKLCRHANPRVLASAVE
                     ALEKLNSESLKPLIVPLLVHPSPGIRSRAIRLLYRWDPREALRHLEATLFADEPQERS
                     AGLFHAFFFPFTEIEPLLRRFLSIEDNPELLQRAGLLFRANPMPEEPSRLLEVMESCR
                     GTKRSLIGELIRGVALSLHQAGLVEKKPETLLQELQEGYRQRKTNELLERCRLALGGT
                     DAEIRRKAVDKLGDLVRLSLTLATDLLRRHLNSETDETIRAQIARILGPETLLPEVES
                     RPPEAAPIPAPIPPTTTRITSEPP"
BASE COUNT          665 a          921 c          812 g          604 t
ORIGIN      
        1 cccccgacaa aaaatgctga tcccctgaag cgagagcgcg agatcgcggt tatcaagcga
       61 atctccgagt atctggccga agaacggtat cggttcgcgc acatcggaga tgattgcatc
      121 gaactctatt tacagggaaa gcatctgacc atgcgcatga tcatctatgc gcacaacggc
      181 catctcgtcg ttcgagtgcc cgagttcatc cgcaatcaga acctcctgtt tcccgcgttt
      241 ctgcgaacca tactcaccgt tcagggggaa tacttcgata tccgattcga gctggccgac
      301 gacggtaaaa gcctttccgc ctgcgccaat cacattctcg aagacaatga gatgaccaga
      361 acccagttca accagtgcat gacggtcgtc gcgttcattg ttgatcagca ctatccactg
      421 tttctcaagg tcttgtacaa ggagcacctc gagtcgggtg aacccatgac cgaagacgaa
      481 aaaaaagccg aaagcgggga gtcggacagt acgccaacca cagacccacc cgacggccac
      541 tctctcaact gacgccctgg ggggcgaaag gacatcgttc atgaatgcca ctaccttcgc
      601 gcaagtccgt cagaccgctg tcacaatcgc atgcatcatc tgcctgctgt tcgcggtttc
      661 ttccgcatgg gcccgcacca ttcagattcc tgcctggtat gagttttcag cagctcttga
      721 agaggttcct cagattgatc aaccagtaca tttcatcgcc tcactcacgg cacttgtcga
      781 cgatattgac aatgtaagcg tctctctcgt gctccccccg ggctggtccg cagacaagac
      841 ctcggacact ctggcgaaac ttcccgcaaa acagacccat ggcttccggt ttctgatcaa
      901 accggccgga ccactgccca atggcggtat cggatgttcc ttccaggccc gagtccccaa
      961 agcagccttg atagagtcgg tccgcgccac cggtcatgac ctcggcgaag cgatggcaca
     1021 ggcaatcgcc aacagtccag atgtcggcaa cgggtacaca gatgtcgcgt tcgcgctctt
     1081 cgccgaagaa gggttttttc cactcggtga aacgatgtgg ttggcgtatg acgaccgttt
     1141 caagccttca gaggcccttc gcggcccctc ggtctggaag gatgagctga tctccactca
     1201 ccaggcggaa accgatgtcg agatgttcga tcgtttgaaa cagcaactga cgtcgaatcc
     1261 ccgactcgcg gagcagttgg cgcgtaatgg tatcgacgtc aaccgcaaac gcggcgacgc
     1321 cctgcacgcc cagctggttc tggcgaccga ggcttatctg aagggcgagt ttccacggtg
     1381 tctcgaatgg ctgacccgtt tcgagggaga gatggccgcg ggagaagccg gtcagcagct
     1441 cgaactgcga atcgccgggc gtaatctgcg ctcactgtgc ctctgggcca gcggcaacaa
     1501 gcgggacgcc gaagagttgc tgcgtgagac gttttatctc aaccgcaagc tccccgtcca
     1561 acgctatgtg ctgcgcaacc tcgggctact cacgttggag aaaggcgacc gcaacacagc
     1621 tcgggaaatg ttccgcctgg ccctggaaat caaacccgca tattccctgc tgaaaaagga
     1681 attcgctctg ctgcagaaaa agtaacggaa ggggtggagc cggcactcac caggttcgcc
     1741 ggtttctgag acagaatgga cgcggttcag acactgttcg ccgatctgcg gtctccggat
     1801 ctgtcgattc ggttttccgt tttgtcacgc attgaaggtc tcgaatggac accggagcag
     1861 attcagacgc tgcgcgagct cttcagcgca gagacagacc ccggaacgcg ctttcatctg
     1921 caaaagattc tcacccgcgt cagcagcggg ctcgagaaaa aagccaacgc cgaagtcgcc
     1981 gttgagctga cctcactcct gcgcaagccc gagagagatg atctcggtct cgcgattctg
     2041 ctcgaacagg tcagccgcaa ggaagcgccg ctggtggcaa caaagctgcg tcatgctgac
     2101 tggacctctt tttctgcgga agtgctgcca ttcgtcattt cattctttcg acgacatggg
     2161 tccccagaag acgttccgac actcgaaaag ctctgccgtc atgccaaccc gcgggtactc
     2221 gcctcggccg ttgaagcgct cgagaaactg aattccgaga gcctgaaacc gctgatcgtg
     2281 cctctgctgg tccacccaag cccgggaatt cgctcgcggg cgattcgcct gctctaccgc
     2341 tgggaccctc gcgaagcgtt acgccacctc gaagcaacgc tcttcgccga tgagccgcag
     2401 gaacggtcgg ccggtctctt tcacgccttc ttttttccgt ttaccgagat cgaaccgctc
     2461 ctgcgacgtt ttctgagtat cgaagacaac ccggagctgt tgcagcgcgc cggtctgttg
     2521 tttcgcgcca acccaatgcc agaagagccg agtcggctgc tcgaagtgat ggaatcgtgc
     2581 cgtggcacca agcgcagtct gatcggcgaa ctgatcaggg gcgtcgctct ctcgctgcat
     2641 caggcaggtc tggtcgagaa gaagccagaa acactgcttc aggagttgca ggaagggtat
     2701 cgccagcgca agaccaatga gttgctcgaa cggtgccggc tggcactcgg aggtacggat
     2761 gctgagattc gtcggaaagc ggtcgacaag ctgggcgacc tcgtacgact gagcctgacg
     2821 ctggcgacag acctgttacg tcgccatctc aattctgaaa cggatgaaac cattcgcgcc
     2881 cagattgcca gaatcctcgg tccagagaca ctgctccccg aagtcgaatc gcgcccaccg
     2941 gaagcggcac ccattcccgc gcccattcca ccgaccacaa ctcgcatcac ttccgaaccc
     3001 cc
//
LOCUS       DAORHD010000547         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37955_length_2999_cov_3.567935, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000547 DAORHD010000000
VERSION     DAORHD010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37955_length_2999_cov_3.567935"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKO06_11845"
     CDS             complement(<1..809)
                     /locus_tag="PKO06_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV70384.1"
                     /translation="MRIIKATSPITPEIALTMGGKGSNLYKLTQSGAAVPPFFVLGSP
                     CFTEFLTGFQAEFERIVDALDCSTPARVAETSRQVREFLCQQRMPAAIAQEAMLALKE
                     IAGEGDFLAVRSSALGEDSKEFSYAGMLDSYLFRKTPEEVQQAVLECWASLYSDRAVA
                     YRHMRGVSQKGLAMAVVVQKMIDGSASGVTFTVNPNTKYADEILISAVPGLGEGLVSG
                     GLDADQFVVLKGDTYAIDTRTVADKVGRVVFDRDKGFGTIEKELPPETRNKA"
     gene            1293..2336
                     /gene="rsgA"
                     /locus_tag="PKO06_11850"
     CDS             1293..2336
                     /gene="rsgA"
                     /locus_tag="PKO06_11850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNV70385.1"
                     /translation="MSYSQSCRRSRVWDPDEDEHVPQIEKIRRPSTPKPVPKYGGANR
                     RPDPTRDGVVKEIGPGRCRTWVDGTDWDCLLPARLLATQKESLAVGDRVRVRPLGGGQ
                     GAIDEVLPRRTVLDRPDPGNRHRRNVIAVNLDLAIIVATLTDPPFRPGLIDRYLVRLQ
                     KAGIDPILCLNKLDVWRELPECDQYDSEVRAYVEIGIPVFRVSAATGEGIDSLRESIL
                     GRTLALIGHSGVGKSSLVNALAPESDRREGHVRSFDGKGRHTTTGSCLIDLGQKTLLI
                     DTPGVRSFGLGSLHWEEVLEAFPEIRHLGRPCRFSDCRHLDEPECAVREAVEQAEIPG
                     GRYSRYRRLVENL"
     gene            2499..2738
                     /locus_tag="PKO06_11855"
     CDS             2499..2738
                     /locus_tag="PKO06_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70386.1"
                     /translation="MRRIKLYAALVGLTIFTLVMNAHYLGFAPAMFRKGNMYFFGNVG
                     HPQDFVKAVEWFTKSLRTSRKNGWNVVYDGLPLQG"
     gene            2775..>2999
                     /locus_tag="PKO06_11860"
     CDS             2775..>2999
                     /locus_tag="PKO06_11860"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF015280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNV70387.1"
                     /translation="MLLAVDIGNTNIKFGVYDAEDLRAKLSVPTIRDISAAGLAKVVG
                     SRVTHTISTAIVSSVVPEVDAAVREFINSSF"
BASE COUNT          621 a          829 c          877 g          672 t
ORIGIN      
        1 gctttatttc gagtctcggg cgggagctct ttctcgatgg tgccgaaacc tttgtcgcga
       61 tcgaagacca cgcggccgac tttgtcggcg acggtgcgcg tatcgatcgc atacgtatcg
      121 cccttcagca cgacgaactg gtcggcatcc agacctcctg agacgagacc ttcgccgaga
      181 ccgggaacag ccgaaatcag gatctcatct gcgtatttcg tgttcgggtt gaccgtaaag
      241 gtgacgcctg aagcagagcc atcgatcatt ttctgcacca cgaccgccat ggcgagccct
      301 ttctggctga ccccgcgcat gtgacggtag gcgacggcgc gatccgaata cagcgaggcc
      361 cagcactcga gcactgcctg ctgaacttcc tctggtgttt tgcgaaacag atagctgtcg
      421 agcatgccgg cgtaggagaa ctctttggaa tcttcgccca gagcgctcga acgaacggcc
      481 agaaaatcgc cttccccggc aatctctttc agggcaagca tcgcctcctg tgcgatcgcg
      541 gcaggcatgc gctgttggca gagaaactcg cgcacctgac gtgaggtttc agctacgcgt
      601 gcgggagttg aacagtcgag agcgtcgacg atgcgttcga actctgcctg gaacccggtc
      661 aggaactcgg tgaagcacgg actgccaagt acgaagaatg ggggaacggc tgctccgctc
      721 tgagtgagct tgtacaggtt gcttcccttg ccgcccatgg tgagagcgat ttcgggggtg
      781 atcggtgaag ttgccttgat gatgcgcata ccgactgact ccttcgtcgt cagaatattc
      841 ggagcaccga cgtttcaggc cagcaaaact cgattcacag cataccagag agactcatac
      901 gtctcaacca ggagttctct ccagatacga ccgaatggaa cctttgctga actccagaca
      961 aactcatgaa tgcgcgaaca aaactccgtt accggccctt ggttcttgcg ttgtggagtt
     1021 tctgcttgac acggtggtgg tatctgcata cattctttat cgatttcgtg tcgcgaattt
     1081 caacaaccac aggaggccag ccatgatgcc gattgctcat gaagtgttca acagactgcg
     1141 cctcactgcg aaaattcgct gacccgcgac cggcactgac tgcggggctg ctccgagtgg
     1201 ggcagccccg tttctttttg cgtggcgcag tctcttcgtc ggtgtttttc ttcccccccc
     1261 tttttttcta gacacgagga gataaccaac acatgtcgta ttcgcaatcc tgtagacgct
     1321 cacgcgtctg ggatcccgat gaagatgagc acgttccaca gatcgaaaag atccgcaggc
     1381 cctccacgcc gaaacccgtt ccgaaatacg gcggcgctaa ccggcgtccc gatccgaccc
     1441 gcgacggggt ggtcaaagag atcgggcccg gcagatgtcg cacgtgggtc gacggcactg
     1501 actgggactg tctgcttccg gcccgcctgc tcgcgacgca gaaagaatcg ctggcggtgg
     1561 gcgaccgggt ccgggtgcgc ccgctcggcg gcggacaggg cgctatcgat gaggtgctgc
     1621 cccggcgaac cgttctcgac cgacctgatc ccggaaaccg ccatcgccgc aatgtgatcg
     1681 cggtcaacct ggatctggcg atcatcgtcg cgactctgac cgatccaccg tttcgaccgg
     1741 gtttgatcga ccggtatctg gtccgtttgc agaaagccgg catcgacccg attctgtgtc
     1801 tcaataaact tgatgtatgg agagaattgc ctgagtgtga ccagtacgat agcgaagtgc
     1861 gtgcgtatgt cgaaatcggc attccggtgt ttcgtgtgtc agcggcgacg ggcgaaggaa
     1921 ttgactcgct gcgtgagtcg attcttggcc gcactctcgc gttgatcggt cacagtggcg
     1981 ttggcaagag ttcgctggtc aatgcgttgg cacctgaatc tgatcggcgc gaaggtcacg
     2041 tgcgatcatt cgacggcaaa ggccggcata ccacgaccgg ttcatgcctg atcgatcttg
     2101 gtcagaagac gctgttgatt gatacccccg gcgttcggtc cttcggcctg ggttcactgc
     2161 actgggaaga agtccttgaa gcctttccgg aaatcagaca tctgggacgc ccgtgtcgat
     2221 tcagcgactg tcgacatctc gatgaacccg agtgcgcggt acgtgaagcg gtcgagcagg
     2281 ccgaaattcc aggagggcga tactcccggt atcgaaggct ggtcgagaat ctgtgacttc
     2341 ggcgtcgctt cagctgtttg cggctgaagc gacgtttggt cacgtgtcga aaccgcatgg
     2401 gtctctccgg aggctgaggt tgatcgaccg tgccgagaag actatactga tcacgagttc
     2461 gctgacaatg agcagcatca tgaacaggag ggtgtcgagt gagacgcatc aagctttatg
     2521 cagcgttggt ggggttgact atttttacac tggtcatgaa tgctcactat cttggctttg
     2581 cgcccgccat gttccgcaaa ggcaacatgt acttttttgg caacgtcggt catccacagg
     2641 attttgtgaa agcggtcgaa tggttcacca aaagcctgcg taccagccgc aagaacggat
     2701 ggaacgttgt ttatgacggg ctgccccttc agggctagtc agaaacacct gcgatagcgg
     2761 gtggtaccga caagatgctc ttagccgttg acatcggaaa caccaacatc aaattcggcg
     2821 tttatgacgc tgaggatctc cgtgccaaat tatctgttcc gacaatccgt gatatctcgg
     2881 cggccggact agcaaaggtc gtcgggagcc gtgtgacgca tacaatttcg acggcgatcg
     2941 ttagttcggt cgtgcccgag gtcgatgcgg cggtgcggga gtttatcaac tcgtcgttt
//
LOCUS       DAORHD010000548         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37956_length_2999_cov_3.555707, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000548 DAORHD010000000
VERSION     DAORHD010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37956_length_2999_cov_3.555707"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PKO06_11865"
     CDS             complement(<1..348)
                     /locus_tag="PKO06_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV70388.1"
                     /translation="MKYIHLDSTKCDGKRSCEKACAQTFFKQSDVGFSCIQIAKTDLG
                     HVANVCNQCGRCIDICPVKAISRNKLGTVMVDKKACIGCFMCIGFCPTQSMRRAHDHR
                     EPFKCVSCGACVKA"
     gene            512..802
                     /locus_tag="PKO06_11870"
     CDS             512..802
                     /locus_tag="PKO06_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015736721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HNV70389.1"
                     /translation="MITHIVFFKLFDRSAESLCRVRDRILEMHGRIPVMKHLEAGADI
                     IRSERAYDVALVAHFESRADLQTYIDHPVHQEFVQFIRPLRESTISVDYEQL"
     gene            871..1290
                     /locus_tag="PKO06_11875"
     CDS             871..1290
                     /locus_tag="PKO06_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70390.1"
                     /translation="MGLIFVPLALMLVLVIVNVMLYLIARFGGWQELAGRFPERTMFQ
                     GRQWRFRSLGLSNWTRYNNAVTIGANAQGIRFSMPWFFAAGHAPFFISWGDIAEWRET
                     KQWFLPVIELSLKGSERVITLPRALALEILRERPAES"
     gene            1287..1874
                     /locus_tag="PKO06_11880"
     CDS             1287..1874
                     /locus_tag="PKO06_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70391.1"
                     /translation="MNSYLMLRKLKEGMIVLAIIGILLAIVVPNLRKATTTERMDACC
                     TFCGARTIKSIEKGGVVSVAPPQLHTAVPTFDHQHFWVPDGKVERTSLFGSSARELHG
                     TKLFVTLLELQTYLGVFGGRKDDSRLMDFYLKQISKLPDSPGPLEIDALRLLMISNQP
                     FAGLKGLTSEETFWKTLEQIDTLIPTIGKPASPHN"
     gene            1882..1977
                     /locus_tag="PKO06_11885"
                     /pseudo
     CDS             1882..1977
                     /locus_tag="PKO06_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011176712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
     gene            1997..2263
                     /locus_tag="PKO06_11890"
     CDS             1997..2263
                     /locus_tag="PKO06_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006420653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNV70392.1"
                     /translation="MAKMMQISYPDQLPDVMNMSQSEFEASARLLLAVKLFEMQRISA
                     GMAAELAGMERVTFLLKLREFRVTMIDLEVDEFENDLRMGERTT"
     gene            complement(2289..2750)
                     /locus_tag="PKO06_11895"
     CDS             complement(2289..2750)
                     /locus_tag="PKO06_11895"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HNV70393.1"
                     /translation="MKPVLLLTVFLWYVTLIILSLRGSRSAYLALIATGLLYFPARVG
                     FKFNPGSVDLSCGGSEIWLALFNYPHVILFALFFFMTSAQFPLNRWSDFVWAGVVTIS
                     FGIAVEIAQGITGNGHCRVRDLIPDTIGVLIGATIKISVQKSKEKSPSTSP"
BASE COUNT          685 a          784 c          837 g          693 t
ORIGIN      
        1 ggccttgacg caggcaccgc aggacacgca tttgaacggt tcacgatgat catgtgcgcg
       61 acgcatcgat tgggtgggac agaagccgat gcacatgaaa cagccgatgc aggctttctt
      121 gtcaaccatg acggtgccga gtttgttgcg cgaaatagct ttgaccgggc agatgtcgat
      181 gcagcgaccg cactgattac agacgttggc gacgtgtccg agatcggttt tcgcgatctg
      241 aatgcacgaa aagcccacgt ctgactgctt gaagaacgtt tgcgcacagg ccttttcaca
      301 ggaccgtttc ccgtcgcact tggtcgaatc gagatggatg tatttcattc ccgaaaaact
      361 ccttgatgat cggctggaca atctctggaa acatgattat tgtgcgtgaa gtctcagggg
      421 aagtcaagaa ttcgctcggc agtcgcactg ctgtggcagc gcgtttgctg agagcacttt
      481 tgctggcgta tcccttgcct ggaggtagtg aatgatcacc cacatcgtat tcttcaaact
      541 cttcgatcgc tctgccgaaa gtctctgccg cgtacgagat cgaattctgg aaatgcacgg
      601 ccggattccc gtcatgaagc accttgaagc gggtgccgac atcattcgtt cggagcgggc
      661 gtatgatgtc gctttggtgg cgcactttga gtcgcgagcc gatctgcaga cctacatcga
      721 tcatccggta catcaggagt ttgtacaatt catcagaccc ctgcgggagt cgacgatctc
      781 ggtagattac gagcagctgt gataggctgg tgtaaaagag ttatccgggt tgcgactcgg
      841 acatctcacg actgaacatg gagatttgac atgggactga tcttcgttcc actggcactg
      901 atgctggtgc ttgtcattgt gaacgtcatg ttgtatctga tcgcccgttt tggcggttgg
      961 caggaactgg ccgggcgatt tccggagcgc acgatgtttc agggcaggca gtggcgcttt
     1021 cgcagtctgg gcctctcgaa ctggacccgt tataacaatg ccgtgacgat tggcgcgaat
     1081 gcccagggaa ttcgcttttc catgccctgg ttctttgccg ctggtcacgc gccgttcttc
     1141 atctcgtggg gggatatcgc cgagtggcgc gagacgaaac agtggtttct tcccgtcatc
     1201 gagctctcgc tcaagggaag cgagcgggtg attactctgc cgcgcgccct ggccctcgaa
     1261 attctgcgcg aaaggcccgc tgagtcatga attcgtatct gatgctgcgc aaactgaaag
     1321 aagggatgat cgtgttggcg atcatcggca ttctcctggc gattgtggtg ccaaacttga
     1381 gaaaggcgac taccacagag cgtatggatg cctgctgtac gttttgtggc gctcggacaa
     1441 tcaagtcgat cgagaaaggc ggtgtcgtct ccgttgcgcc accgcagctt catacggctg
     1501 ttccaacgtt cgatcatcag cacttctggg tccctgatgg caaggtggaa cgcacgtcgc
     1561 tttttggcag cagcgcccgg gaactgcacg gcacaaaact cttcgtgact ctgctcgagc
     1621 tgcagacgta tctcggggtt ttcgggggcc gcaaggacga ctcgcggctc atggactttt
     1681 acctgaagca gatctcgaag cttcccgatt cgccggggcc gctggaaatc gatgcgctcc
     1741 gccttctgat gatcagcaat caaccgtttg cggggctaaa ggggctgacc tccgaagaaa
     1801 ccttctggaa gacgctcgaa caaatcgaca ccctgattcc caccatcggc aaacccgcat
     1861 ccccccacaa ctgatcttct acttcgattc tttttcctcg gcgcgaatga acgacctcgc
     1921 atcgagctta ttggtgccaa agccgcatat gacccggaag gacctctgat cctgtagatt
     1981 ggtagtgagg tgggaaatgg cgaaaatgat gcaaattagt tatccggatc aactccctga
     2041 tgtgatgaat atgtcgcaga gcgagtttga agccagcgcg cgccttttgc tcgctgtgaa
     2101 actctttgaa atgcaaagaa tctctgcggg aatggccgcc gaacttgcgg gaatggaaag
     2161 agtgactttt ctgctgaaac ttcgagagtt tcgcgtcacc atgatcgacc tggaagtgga
     2221 tgagttcgag aatgacttga gaatgggtga acgtacaacc tgaccgcgaa cccaccgcga
     2281 tcataatctt acggagaggt tgacggactc ttctccttgg atttctgaac agagatctta
     2341 attgtggctc caatcaatac tcctatcgtg tcagggatca ggtcacgtac gcgacaatga
     2401 ccgttaccgg taattccttg ggcaatctcg accgcaatgc cgaagctgat tgtcaccacg
     2461 cctgcccaga caaagtcaga ccaccgattc aatggaaatt gcgcactggt catgaaaaag
     2521 aaaagggcga acaggatgac atgagggtag ttgaacaggg ccagccagat ttctgacccc
     2581 ccgcagctca ggtccaccga ccctgggttg aacttgaacc ccactcgggc tggaaaatac
     2641 aacaggccgg tggcgatcaa cgcgagatat gccgaccgtg aaccccggag tgacaatatg
     2701 atgagcgtga cataccaaag aaacaccgtc aaaaggagaa ctggcttcat caaccgatat
     2761 accctttcct ggatgagact ttcaccacct cagataacac catcacatgt cgaacctctg
     2821 attcgaaaga ccttacgttc ttcagacgag agtatcggga gcgaccagct cgactttgag
     2881 gcggtcgggg tcttcgaaga agacggcgta gtagccggca ccgcccgcgt acgggtgacg
     2941 atcggcgtag agaatcgtga cgtttcgttg gcgcagggct tccgtcatgg tgtcgacct
//
LOCUS       DAORHD010000549         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37957_length_2999_cov_3.353940, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000549 DAORHD010000000
VERSION     DAORHD010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37957_length_2999_cov_3.353940"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKO06_11900"
     CDS             complement(<1..1292)
                     /locus_tag="PKO06_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNV70394.1"
                     /translation="MQIDKSKIQSILATDCGSTTTKAILIEKREGEFRLIVRGEAPTT
                     VEAPVEDVTSGVLNAVSEVQELVGRQLLSDDGTKLIKPRQGEKGVDLYVSTSSAGGGL
                     QMMVAGVVKSMSGESAERAALGAGAIVMDVIATNDGRLPHQKIQRIRQLRPDMILLSG
                     GVDGGTTSHVAELGEIIAAANPKARLGIGYELPVLYAGNKDARDLIKERLADKVALDI
                     TDNLRPSMEKENLMPARHHIQHLFLEHVMSHAPGYPRLMEMTKPVDIMPTPGAVGDIM
                     QLISKKKGITVLGVDIGGATTDVFSVFQGIYNKTVSANYGMSYSISNVFAEAGLDNVM
                     RWVPFDMDERDLRNRVKNKMIRPTSIPSMVQELVVEQACAREALRLSFDQHKALAVGL
                     KGVQQERSIGDVFDQTMTGETLVSLKALDMLIGSGGVLS"
     gene            complement(1311..1763)
                     /locus_tag="PKO06_11905"
     CDS             complement(1311..1763)
                     /locus_tag="PKO06_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70395.1"
                     /translation="MTESAETGPVTPSAVAASAASAVSSPVCDTNVQTATTVVDAGTS
                     AGGPPATSEGLIGQGLHRTYSDEELQTFIQSVAREIVSRGMSVPAVFALELMKPLSFI
                     SASSLTFMGPILETLIDPQKIEKMSCVLEDRNRVEALLVAIETLEQQK"
     gene            complement(1767..2426)
                     /locus_tag="PKO06_11910"
     CDS             complement(1767..2426)
                     /locus_tag="PKO06_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70396.1"
                     /translation="MATLTIWAAAAITLCIFSFLWQDNRAYRFAEHLFVGVSAGYYFG
                     SIAIHQVLKPNLLAKLFPATFAVGEAAQADPNYMLLVPALLGVLMIFRLSANYSWVSR
                     FTVAFVMGISTGLTIVYSTQQMLIPQMKKAVVPLFVQNDLFMTFSNWVLVIGSIACIF
                     YFFFSTEHRGPVFGTVSKVGIWYLMISFGAAFGATVMGRISLLIGRFHFLLHDWLGLV
                     K"
     gene            complement(2439..>2999)
                     /locus_tag="PKO06_11915"
     CDS             complement(2439..>2999)
                     /locus_tag="PKO06_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70397.1"
                     /translation="AAVLKLKESADPKDKAIGEKLLSGRDFVNLGYKPGAIIQIRRII
                     ADFLSPYPVDANGKPTSEMEIFKHADGRKFSIKDIGIIVSFTAGTGGIESFISVKSEH
                     ERPMAAGCTSVNIPRFYTYIQTKQLVGMTGGMPGAAEYENLIKIKGTATSGMDSQSIC
                     HLVIIAFIILGNLAYIAEQRKAARKA"
BASE COUNT          635 a          886 c          841 g          637 t
ORIGIN      
        1 gacaacacgc cgcccgagcc gatcagcatg tcgagggcct tcaagctgac cagcgtttca
       61 ccggtcatgg tctggtcgaa tacgtcgccg atgctgcgtt cctgctgcac gcccttcaat
      121 ccgacggcga gagctttgtg ctgatcaaac gagagacgca acgcttcacg ggcacacgcc
      181 tgctcaacca cgagttcctg aaccattgat ggaatcgagg tcgggcggat catcttgttc
      241 ttgacgcgat tgcgcagatc gcgttcatcc atgtcaaacg gcacccagcg catgacatta
      301 tcgagccccg cttcggcaaa cacgttgctg atcgaataac tcatgccata gttggccgac
      361 acggtcttgt tgtaaatgcc ctggaacacc gaaaacacgt ccgtcgtggc cccaccgata
      421 tcgacgccga gaacggtgat tcccttcttc ttcgagatca gctgcatgat atcgccgacc
      481 gcgcctggtg tcggcatgat gtcgacaggc ttggtcatct ccatcagtcg cggatacccg
      541 ggcgcatggc tcatgacgtg ttcgagaaac aggtgctgaa tgtgatgccg ggcgggcatc
      601 aggttttctt tttccatcga agggcgcagg ttatcggtaa tatcgagcgc caccttgtcg
      661 gccaatcgct ctttgatcag atcgcgcgca tccttgttgc cggcatagag taccggaagc
      721 tcatagccga tacccagacg ggccttaggg tttgcggccg caataatctc tccgagttcc
      781 gccacgtgcg aggtcgttcc accgtcgacg ccgcctgaga gtagaatcat gtcggggcgc
      841 aactggcgaa tgcgttgaat cttctggtgg ggaagccgac catcattggt cgcgatgacg
      901 tccatgacga tcgcgcccgc gcccagagcc gcgcgctccg ccgattcgcc ggacatcgat
      961 ttgacgaccc ctgcgaccat catctgcaga ccaccgccgg ccgaactggt cgacacatac
     1021 aggtcgacgc ccttttcgcc ctggcgaggt ttgatgagct tggttccatc gtcgctgagc
     1081 agctgtcggc caacgagttc ttgtacttcc gagacggcgt tgagcacacc gctcgtcacg
     1141 tcctcgaccg gagcctcgac cgttgtcggt gcttcgccac gcacgatcag gcggaactcg
     1201 ccctcgcgct tctcgatgag aatcgccttg gtggtcgtac tgccgcaatc ggttgccaga
     1261 atgctttgaa tcttggattt atcgatctgc acagcgtgct tcccctttcc ttatttctgc
     1321 tgttccagag tttcgatcgc aaccagcagt gcttctacgc gattgcgatc ctccagaacg
     1381 caggacatct tttcgatctt ctgcgggtcg atcagagttt ccagaatcgg ccccatgaac
     1441 gtgagtgacg aagcgctgat gaacgaaaga ggcttcatca gctcgagtgc gaacactgcc
     1501 ggaacgctca tgccgcggct gacgatctcg cgcgcgacac tctgaatgaa cgtctgcagt
     1561 tcttcgtcag aataggtgcg atgcagcccc tgtccgatca agccttcaga ggttgccgga
     1621 ggtccgccgg cgctcgttcc ggcatcgacc accgtcgtgg ccgtctgcac attcgtgtcg
     1681 cagacaggtg acgacactgc tgatgccgcg gacgcagcga cagccgacgg cgtcaccgga
     1741 ccggtctctg ccgattcagt catcgatcac ttcaccagtc cgagccagtc gtgcaacaga
     1801 aagtgaaatc gaccgatgag cagcgaaatg cggcccataa ccgtggcccc aaacgcagcg
     1861 ccgaagctga tcatcagata ccagatcccg acctttgaca cagtaccgaa gaccgggccg
     1921 cgatgttccg tgctgaagaa gaagtagaag atacaggcaa tcgaaccgat caccagcacc
     1981 cagttcgaga aggtcatgaa taggtcgttc tgcacgaaca ggggcacaac cgctttcttc
     2041 atctgcggaa tcaacatctg ctgcgtcgag tagacgatcg tcaggccggt gctgatgccc
     2101 ataacgaagg ccaccgtgaa ccgactgacc caggagtagt tggcactgag gcggaagatc
     2161 atgagcactc ccagtaacgc gggtaccagg agcatgtagt tcggatcagc ctgagcagcc
     2221 tctccgacgg caaaggttgc cgggaaaagc ttggccagca gattcggctt gagaacctga
     2281 tgaatggcaa tcgacccgaa gtagtatccg gccgacacac cgacgaacag gtgttccgca
     2341 aaccgatacg cccggttatc ctgccagagg aaactgaaga tgcacagagt gatggctgca
     2401 gcagcccaga tagtcaaggt agccatagtg ttcctccgtt aggctttccg cgcagcttta
     2461 cgctgctcgg cgatgtaagc gaggttgccg agaatgatga aggcgatgat caccagatgg
     2521 cagatggact gtgaatccat gcccgaggtg gccgttccct tgatcttgat caggttctca
     2581 tactcagcgg cacctggcat tccgcctgtc atgccgacca actgcttggt ctgaatgtag
     2641 gtgtagaagc gcggaatgtt gaccgacgtg cagccagcgg ccatgggtcg ttcgtgttcg
     2701 ctcttcacgc tgatgaacga ttcgataccg ccggttccgg cagtgaagct gacgatgatg
     2761 ccgatatcct tgatcgagaa cttcctcccg tctgcgtgct tgaagatttc catctccgag
     2821 gtcggcttgc cattggcgtc gacggggtaa ggagacagga agtccgcaat gatgcggcgg
     2881 atctgaatga tcgccccggg cttgtacccg aggttcacga aatcacgccc gctcagcagt
     2941 ttctcaccga tcgccttgtc tttcgggtcg gccgattctt tcagcttgag cacagcagc
//
LOCUS       DAORHD010000550         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37958_length_2999_cov_3.284307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000550 DAORHD010000000
VERSION     DAORHD010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37958_length_2999_cov_3.284307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKO06_11920"
     CDS             <1..233
                     /locus_tag="PKO06_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70398.1"
                     /translation="RGTGAEEIDGGMEFNGLYLYHPRYRQTTVKSWWWVEKDTFLISF
                     GMVPGYSVVRRYPFTRLFPPRHQSILVLRKTP"
     gene            344..748
                     /locus_tag="PKO06_11925"
     CDS             344..748
                     /locus_tag="PKO06_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70399.1"
                     /translation="MYRKQMTPRAGGFGLYIVTVLFVTALCVIGLFYPQFFRMRSGIY
                     KISCKEIRRKVEVAVENYAVNNTRSLVQPGKNIDLDMLNGTGFLGEVQYCPEGGKLQY
                     GPQGEVLCTVHRPRVEVTREAEPAGSEKKEGK"
     gene            752..1393
                     /locus_tag="PKO06_11930"
     CDS             752..1393
                     /locus_tag="PKO06_11930"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV70400.1"
                     /translation="MGKHRRRTYLIKTGLQLRYMGIIVSTMLLVAFGVGWIIYYTSWS
                     RISDTPDLTIDKLAEIFDQVNGVLLKWVAVFLLIIASLSIFVSHKIAGPVYRFERLAR
                     VITKGDLTQSVRLRSGDELRELQDAFNSMTDSLRRMVTKDREVIAKLVAAGNKLNDVM
                     KKKKQDPAEIESVAQELYGIIEELRNVTAGFRIDKELASETTSTEGSSEEEEA"
     gene            1412..2434
                     /locus_tag="PKO06_11935"
     CDS             1412..2434
                     /locus_tag="PKO06_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70401.1"
                     /translation="MIRRIWQSSRSSRWLFALLAILVIVGLNGAKPKKKAKSAKKKPA
                     VSAVSQKDPPRVFKKSVPDGRPWYEQMVSDGVFRIAVFWGWDSPREVVEGVFPAFETL
                     NGKSVYYQGRKARIEIGLVTQVSENPREMFQAALEDPSVDVVIYSGHARYGAGPAFGD
                     RDDIFRCGDGQMVEDRHTKPFRRFKATSEDLDQTTFPQTYRIIMLNCCDSSGHFRKTW
                     NRRLRECGAATDLITVEFPVFNMYDHVRLLNLIRDLLQFADWKTIKAHYDAEIHKREN
                     FLVVEPVFIPSVPVAETEEDFESAPEDDHISQIPAVETLPATAVAAPTPRPDSHLLTQ
                     VVTPEP"
     gene            2495..>2999
                     /locus_tag="PKO06_11940"
     CDS             2495..>2999
                     /locus_tag="PKO06_11940"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNV70402.1"
                     /translation="MGNLSQTNNHTGRFTTSAIAFLALIGVGTIGYKLIEGPHWTWLD
                     SLYMTVITLGTIGYGEVHTLGTGGRLFTVCLILMGAGVMAYTVTAMAQFVVEGKLQEL
                     WGKKRMKDRIDALKDHYVVCGAGDTGLVVIGELTRRKIPFVVVESNPKVAESLIEGDI
                     LVVEGDAT"
BASE COUNT          648 a          795 c          923 g          633 t
ORIGIN      
        1 cccgggggac cggtgccgag gaaattgacg gtggcatgga attcaatggg ttgtatctgt
       61 accatccgag gtatcggcag accacggtga aaagctggtg gtgggttgag aaagatacgt
      121 ttctgatcag tttcggcatg gttccgggct attccgtcgt gcggcgttac cccttcacgc
      181 gtctctttcc gccgcgtcat cagtccattc tggtcctgcg gaaaactccg taagtggtat
      241 catggaagag gatcgccgca gggctttgct gatccggatc aggcgaaagc tcaagaaacc
      301 ggcctaactg gtcgatgaag gtggcaggaa ctctggaggt cgtatgtacc gtaaacaaat
      361 gacaccccgt gccggcggtt tcgggctgta tatcgtgact gtgctgtttg tgaccgcatt
      421 gtgcgtgatt ggcctgtttt atccgcagtt ctttcgcatg cgatcaggca tttacaaaat
      481 ttcgtgcaag gaaattcgcc gcaaggtgga agtggcggtc gagaactacg ctgtgaacaa
      541 cactcggtcg ctggtgcagc ccggcaagaa catcgatctc gatatgctga atggcaccgg
      601 gtttctgggc gaggtgcaat actgccctga agggggcaag ctgcagtatg ggccgcaagg
      661 cgaggtgctc tgcaccgtcc accgaccgcg ggtcgaagtc actcgcgagg ccgaaccagc
      721 cggttcggaa aagaaggaag ggaagtgaac catgggaaaa catcggcgtc gcacatacct
      781 gatcaagact ggtctgcagt tacgttatat gggaatcatc gtcagcacga tgctgctggt
      841 tgccttcggc gtgggttgga tcatctacta cacctcgtgg agtcgcatca gtgacacgcc
      901 tgacctgacg atcgacaagc tggccgagat cttcgaccag gtcaatggag tgctgctcaa
      961 gtgggtggcc gtgttcctgc tgatcattgc ctcgctctcg atcttcgtct cacataagat
     1021 cgccggccct gtctaccgtt tcgagcgtct ggcgcgggtg atcaccaagg gcgatctgac
     1081 ccagagcgtg cgcctgcgca gcggcgatga gttgcgcgaa ctgcaggatg ccttcaacag
     1141 catgaccgat tcgctgcggc gcatggtgac caaggatcgc gaggtgatcg ccaagctggt
     1201 cgcggcgggg aacaagctga acgatgtcat gaagaagaag aaacaggacc cggctgagat
     1261 cgaaagcgtg gcccaggaac tctatggcat catcgaagaa ctccgaaatg tcaccgccgg
     1321 attccgcatt gacaaagaac tcgcatccga gacaaccagt acggaaggct cgagcgagga
     1381 agaagaagcc tgaggtcggg agaacgatcg catgatccgg cgaatctggc aatcctctcg
     1441 ctcatcgcgc tggctgtttg ctctgctggc gattctggtg atcgtcgggt taaatggcgc
     1501 caagccgaag aagaaggcga aaagcgcgaa gaagaagccg gcggtgagcg cggtcagcca
     1561 gaaagaccct ccgcgtgtct ttaaaaagtc cgttcccgac ggccgcccct ggtatgagca
     1621 gatggtctcc gacggcgtgt ttcgtattgc cgtgttctgg ggctgggatt ccccgcgcga
     1681 ggtcgtcgaa ggggtgtttc cggcttttga aacgctcaac ggcaagagcg tctattatca
     1741 gggtcggaag gcacgtatcg aaatcggtct ggtaacccag gtttccgaga acccgcgtga
     1801 aatgtttcag gcggctctcg aagacccgtc ggtcgatgtg gtcatctatt cggggcatgc
     1861 tcggtacggg gccggaccgg cgtttggtga ccgcgatgac atttttcgct gtggcgacgg
     1921 gcagatggtc gaagaccgcc ataccaaacc cttccgtcgt ttcaaggcga cctcggaaga
     1981 cctcgaccag accacgtttc cgcagacgta tcggatcatc atgctcaact gctgcgattc
     2041 gtcggggcac tttcggaaaa cctggaaccg gcggctgcgg gaatgtggag ccgcgaccga
     2101 tctgattacc gtcgagttcc cggtattcaa catgtacgat catgtgcgcc tgttgaatct
     2161 gatacgcgat ctgctgcagt tcgctgactg gaagacgatc aaggcgcact atgatgccga
     2221 gattcacaag cgcgagaatt ttctggtggt cgagccggtc tttatcccga gtgtaccggt
     2281 cgcggaaacc gaagaagatt tcgaatcagc acctgaagac gaccacattt cgcagattcc
     2341 agccgtcgag actctcccgg caacggccgt ggcagcaccg acccccaggc ccgactctca
     2401 cctgctgacg caggtggtga ccccggaacc ctgattcaca gaagggctgg ttgcgcgcac
     2461 cgcgcgattc gagtaccatt cgtcaggagc gcgaatgggc aacctttctc agacgaacaa
     2521 tcataccggt cgttttacca cgtcggctat tgcctttctc gcgctgatcg gtgtcgggac
     2581 catcgggtac aaactgatcg aaggcccgca ctggacctgg ctggactcac tctacatgac
     2641 ggtcatcacc ctcggcacca tcggctatgg agaggttcat accctgggca cgggtggacg
     2701 ccttttcacc gtgtgtctga ttctgatggg cgcgggagtc atggcctata cggtgaccgc
     2761 catggcacag ttcgtcgtgg aagggaagct gcaggaactc tgggggaaga agcgcatgaa
     2821 agatcgaatc gatgctttga aagaccatta tgttgtctgc ggcgctggtg acacggggtt
     2881 ggtggtgatc ggcgagttga cccgccgcaa gattcctttc gtggtcgtcg aatccaaccc
     2941 gaaagtcgcg gaaagcctga tcgaaggcga tattctcgtg gtagaggggg atgcgacca
//
LOCUS       DAORHD010000551         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37971_length_2998_cov_4.017669, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000551 DAORHD010000000
VERSION     DAORHD010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37971_length_2998_cov_4.017669"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PKO06_11945"
     CDS             complement(<1..1322)
                     /locus_tag="PKO06_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70403.1"
                     /translation="MTNNLQELLAYKKAENDRKSGNYEAALTYFIAAFNANPQPDTAW
                     RRLYCLRRLGRFEEADQLGTQLVAQFPGSTMVRAERAWLIYDRDVRNPAFKDDPARRL
                     EAVYRMLEYGDDPFVARKAVMEGAKAAREMQAWEQVIELCDRVQAKDFEANPRLINGR
                     KIPGDREIYYFARLKALMQLKRFEETRQLALTAQNEFPFRVDYPRYAALALEGLGQTG
                     QAIEELRRLAAKPKTQWYVLADLARLLVAADKPEEAWPVTCRAAASFGEPSAKVNLYT
                     LMARITEVQGQPDVAARHALLSARVREEQGWRLPNDLSDLLLRLPLPEPHPDRRDLEH
                     LCRADWEAALSGVDHGASTASAGAMTYGSMPGGAVSATAAAGAQRDPSQEEVLKTNVP
                     GTVIIRQPEASFAFIRTESFSEPVFVLVKDLPEAARHDYDKRVHAHLIR"
     gene            1512..>2998
                     /locus_tag="PKO06_11950"
     CDS             1512..>2998
                     /locus_tag="PKO06_11950"
                     /inference="COORDINATES: protein motif:HMM:NF040561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type A1"
                     /protein_id="HNV70404.1"
                     /translation="MTNTHELVHTARRLQGSGRAAEALAVLEQAVREGATQPSLLALR
                     ARLLSQLESPEEGRKQLVELVQAHPDDAELFLDLLSLHLDAGEADEVQRLATQGLESF
                     PRHPGITAALIDALDLLNQPAAAEETARQALEAHPHHPDLERRLRQLTEAANRQKRLQ
                     ETVHEAGRAAQHLQRRIPTDPAFLADFLDVFSGREGVHALQFQTKGGDWGYRPVHTTL
                     TEEKLKRHLAGDETLGLYLVRHDNTSRVMVFDLDITKPFIARFLRDPLERRRLNALLE
                     QEGRRLLFQAKEIGLPLLCEHSGFKGLHFWAIAEAPIPARQWRAVGQWLLDKLDRLPR
                     ELAWELFPKQDTVPEDGLGNLVKIPLGIHRKTSNRSWFLDAETLKAADDQQAAYFDYQ
                     RLSPQTFSDILGRLTLTHLIDETRRENADRSAEQASHASAPTFGSADGRPAGDSALSS
                     RPPLSGPPCELAIRIPLPPRFPERIEQVLAGCRVTWALVEQVLAQR"
BASE COUNT          555 a          989 c          885 g          569 t
ORIGIN      
        1 cggatcaggt gggcatggac gcgtttatcg tagtcgtgac gggcggcttc gggaaggtct
       61 ttgacaagga cgaataccgg ctcagagaag gactcggtgc ggatgaaggc gaacgacgct
      121 tcaggctgcc gaatgatcac ggtgccggga acatttgttt tcagaacctc ttcctgtgac
      181 gggtctcgct gcgcaccggc cgcggcggtc gcactcaccg caccgccggg catcgaaccg
      241 taggtcatcg ccccagcaga tgccgtcgac gcaccatgat cgaccccact caacgccgct
      301 tcccagtctg cacggcagag gtgttcgaga tcgcgccggt cggggtgcgg ttcgggcaac
      361 ggcaggcgca acagcagatc ggaaagatcg ttcggcaacc gccacccctg ctcttcacgc
      421 acgcgagccg acaggagggc atgtctggcg gcgacgtcgg gttgtccctg cacttccgtg
      481 attcgcgcca tcagcgtgta caggttcacc ttggccgaag gctcgccgaa actcgcggcc
      541 gcgcggcagg tgacgggcca ggcttcctcg ggtttgtcgg cggcaacgag caggcgggcc
      601 agatccgcca gcacgtacca ctgggttttc ggcttcgccg ccagccgtcg caactcctcg
      661 atcgcctggc cggtctgtcc gagcccttcg agagccagag ccgcatagcg cgggtagtcg
      721 acacgaaaag gaaactcatt ctgagctgtc agcgccagct gtcgggtttc ttcgaaccgc
      781 ttcaactgca tcagggcctt gagacgcgcg aagtagtaga tctcacgatc gcccgggatc
      841 ttgcggccat tgatcagtcg tggattcgct tcaaaatcct tcgcctgcac gcgatcgcac
      901 agttcaatca cctgttccca ggcctgcatt tcacgcgcgg ctttcgcgcc ctccatgact
      961 gctttacgcg ccacgaacgg gtcgtcaccg tattcgagca tccgatagac ggcttcgagt
     1021 cggcgcgccg ggtcgtcctt gaacgccggg ttgcgaacgt ctcggtcgta gatcaaccac
     1081 gcgcgctcgg cgcgaaccat cgtcgaaccg ggaaactggg cgacgagctg ggttccaagc
     1141 tggtcggcct cttcgaaacg tccgagtcga cgcaggcagt acaagcgacg ccatgcggta
     1201 tcgggctggg ggttcgcgtt gaacgccgcg atgaaatacg tcagcgccgc ttcgtagttc
     1261 ccgctcttgc ggtcgttttc cgccttctta tacgccagca gttcctgaag attgttcgtc
     1321 atagacgtct ccttttccga gttacgtgag ctggttcgtt gtccgaatgg ttcgcggtca
     1381 atttcaagaa ggcggcttcg tcgaaacggt tcaattttcc ttcatcgatt tcgtacgcgc
     1441 atcattattg aacccgcggc ggccgaccgc ctttctttcc tatggaagac atcaacaggc
     1501 gaggctcacc gatgacgaac actcacgaac tggtacatac cgcccggcga ctgcaagggt
     1561 cagggcgtgc cgccgaagct ctggcggtcc tcgagcaggc tgtgcgcgaa ggcgcgaccc
     1621 aaccgtctct tctggctctg cgcgctcgcc ttctcagcca actggaatcg cctgaagaag
     1681 gaaggaaaca gctcgtcgaa ctcgtacaag cgcaccctga tgacgccgaa ctgtttctcg
     1741 atctgctctc tctgcatctc gacgccggag aagcggatga agttcaaagg ctggccacgc
     1801 agggtctcga atcgtttccg cgacaccccg gcatcaccgc cgctctcatc gacgctcttg
     1861 atctgctgaa ccagccggcc gcggccgagg aaaccgcgcg tcaggccctc gaagcgcacc
     1921 cgcaccaccc cgatctcgaa cgccgcctgc gccagttgac cgaagcggcg aatcgtcaga
     1981 aacgcctgca ggaaaccgtg cacgaggccg gccgagccgc acagcatctg caaaggcgca
     2041 tccctaccga cccggcgttc ctcgccgact ttcttgacgt cttctccggg cgcgagggag
     2101 ttcacgcgct ccagttccag accaaaggcg gagactgggg ataccggccg gtacacacga
     2161 ctctgaccga ggaaaaactg aaacggcacc tcgccggcga cgagaccctg gggctgtatc
     2221 tggtgcgaca cgacaatacc tctcgggtca tggtcttcga cctcgacatc acgaaaccgt
     2281 tcatcgcccg cttcctgcgt gacccgctcg aacgacgccg cctgaacgcc cttctcgagc
     2341 aggaagggcg tcgcctgctg tttcaggcga aagagatcgg actcccgctg ttgtgcgagc
     2401 actcgggctt caaggggctt cacttctggg cgatcgccga ggcaccgatt ccggcacgac
     2461 agtggcgggc cgtcggacaa tggctactcg acaaactcga ccgtcttccg cgcgaactgg
     2521 cctgggaact cttcccgaag caggacaccg tgccggaaga cgggctcggc aacctggtca
     2581 agattcccct cggcattcat cgcaagacgt ccaaccgcag ttggtttctc gacgccgaga
     2641 ccctgaaggc ggccgacgat cagcaagctg cgtatttcga ttatcagcgg ctttcaccgc
     2701 agactttctc ggacattctc ggtcgcctga cgctcacgca tctcatcgac gagacccgac
     2761 gcgagaatgc cgatcgctct gccgagcaag catctcacgc gagtgcgccg acgttcggct
     2821 ccgccgacgg ccgccctgcg ggcgattccg ctctctcctc gcggccaccg ctctcggggc
     2881 ctccttgcga gctggcgatc cgcattcccc tcccgccccg gtttcccgaa cgtatcgagc
     2941 aggtgctggc cggatgccgc gtcacctggg cgctggttga gcaggttctg gcgcaacg
//
LOCUS       DAORHD010000552         2997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_37992_length_2997_cov_3.753229, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000552 DAORHD010000000
VERSION     DAORHD010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37992_length_2997_cov_3.753229"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKO06_11955"
     CDS             complement(<1..393)
                     /locus_tag="PKO06_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNV70405.1"
                     /translation="MPKHSKRFNKVVKEHRPAPKLHSVEEAFQGLCKAATAKFDETVD
                     VAIRTGLDPKYADQQLRGAVVLPHGIGKTTRVVVFAAGDKAKEAEAAGADFVGAQDLV
                     TKIEGGWTEFDAAVATPDMMKFVGKLGRI"
     gene            complement(456..881)
                     /gene="rplK"
                     /locus_tag="PKO06_11960"
     CDS             complement(456..881)
                     /gene="rplK"
                     /locus_tag="PKO06_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNV70406.1"
                     /translation="MAKKVVGLVKLQIPAGKANPAPPVGPALGQHGVNIMDFCKNFNA
                     KTQKEGDSIIPVIITIYSDRSYTFETRTPPVSFLLKKAAKIEKGTGTSGKATCGQVTM
                     AQVKEIATKKMPDLTAGSIEAAMRTVKGTARSMGLKVVD"
     gene            complement(899..1450)
                     /gene="nusG"
                     /locus_tag="PKO06_11965"
     CDS             complement(899..1450)
                     /gene="nusG"
                     /locus_tag="PKO06_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNV70407.1"
                     /translation="MIDILKGKWYVIHTQSGAEDKVRQNLEHRISLLGKGNCIFRVVV
                     PKESTERVQDGERVTQSRKIYPGYVLVQMDLDDDTWTVVKNTPGVTGFVGLGEKPTPL
                     HEKEVQNVLRTAGLIDSQRSQAMTPIGFSEGQTVKIIDGPFTDFIGVIKEINVEKRKA
                     KVLVFIFGRDTAIEVDLIQLEEA"
     gene            complement(1478..1693)
                     /gene="secE"
                     /locus_tag="PKO06_11970"
     CDS             complement(1478..1693)
                     /gene="secE"
                     /locus_tag="PKO06_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNV70408.1"
                     /translation="MEKLRLMWKDFSRYLQEVWIEVRPNKGRVVWPAYENVKLSTKAV
                     VVSSIFLGLFIGVMDYLFAAMLKFLVS"
     gene            complement(1725..1797)
                     /locus_tag="PKO06_11975"
     tRNA            complement(1725..1797)
                     /locus_tag="PKO06_11975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1762..1764),aa:Trp,seq:cca)
     gene            complement(1835..1984)
                     /gene="rpmG"
                     /locus_tag="PKO06_11980"
     CDS             complement(1835..1984)
                     /gene="rpmG"
                     /locus_tag="PKO06_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNV70409.1"
                     /translation="MREQINLVCTSCKRKNYSSQKNKRNDPDRIEMKKYCPAERKHTL
                     HKEGK"
     gene            complement(2090..>2997)
                     /gene="tuf"
                     /locus_tag="PKO06_11985"
     CDS             complement(2090..>2997)
                     /gene="tuf"
                     /locus_tag="PKO06_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNV70410.1"
                     /translation="AQMDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKCDL
                     VDDPELLDLVELEIRDLLSKYEFPGDKTPVIRGSATEAIAGAGKYGPQAIHNLVKALD
                     EYIPLPEREVDKPFLLAVEDVFTITGRGTVVTGRIERGMVKVGDAIEIVGLREENKNS
                     VVTGVEMFRKILESGQAGDNVGVLLRGINKEDVERGMVLCKPKSIKPHKKFMGQVYIL
                     TKDEGGRHTPFFTGYRPQFYFRTTDVTGTCTLTEGKEMCMPGDRTEVIGELITPIACE
                     AGLRFAIREGGRTVGAGVVTKILDE"
BASE COUNT          572 a          863 c          855 g          707 t
ORIGIN      
        1 gatgcggccg agttttccga cgaacttcat catatcgggg gtcgcaacgg cggcgtcgaa
       61 ttccgtccag cctccttcga ttttggtgac cagatcctgg gcaccgacga agtctgctcc
      121 agcggcttcc gcttctttcg ccttgtcacc ggcggcgaaa acgacgaccc gagtggtctt
      181 tccgattccg tgcggcaaca caacggctcc acgaagctgt tgatcagcgt atttcggatc
      241 gagaccggtg cgaatcgcaa catcgacggt ttcgtcgaac ttcgcggtcg cggccttgca
      301 cagaccctgg aacgcctctt caacggagtg cagtttcgga gccggacgat gctccttcac
      361 cactttgtta aagcgctttg agtgtttcgg catgctcccc tcctttcttc aaaactcacg
      421 cgatccgggg aggaatcgcg ggaatcaaat acgaattaat cgacgacctt cagccccatg
      481 ctgcgcgccg tgcctttgac ggtgcgcatc gccgcttcga tggacccggc ggtaaggtcg
      541 ggcatctttt tggtggcgat ttccttcacc tgagccatcg tgacctggcc acaggtggcc
      601 ttgcccgagg ttccggtgcc cttctcgatc ttcgccgctt ttttcagcag gaaggagacg
      661 ggaggcgtgc gggtttcgaa ggtgtacgac cggtcactgt agatcgtgat gatgaccgga
      721 atgatcgaat cgccttcttt ctgggttttg gcgttgaagt tcttgcagaa atccatgatg
      781 ttcacgccat gctgacccag agccggaccg accgggggag ccggatttgc cttaccggcg
      841 ggaatctgca acttgacgag accgactact ttcttggcca tagtactact ccttcagatc
      901 aggcctcttc gagctgaatc aggtcaactt cgatcgcggt gtcacgcccg aagatgaaga
      961 ccagcacttt agcctttcgt ttttcgacgt tgatttcctt gatgaccccg atgaaatccg
     1021 tgaacggccc atcgatgatc ttcaccgtct ggccctcgga gaacccgata ggcgtcatgg
     1081 cctgcgaccg ctgcgagtcg atcagaccgg ctgtgcgcag aacattctgc acttcttttt
     1141 cgtggagagg cgtgggtttc tcgcccaggc cgacgaaccc cgtgacaccc ggcgtgttct
     1201 tcacgaccgt ccaggtgtcg tcatcgagat ccatttgtac cagcacgtaa ccggggtaga
     1261 tcttgcggct ctgcgtcaca cgctcgccgt cctgcacccg ctctgtgctt tccttgggaa
     1321 caaccacacg gaaaatgcag ttccctttcc ccagcaggga aatgcggtgt tcgaggttct
     1381 gacgaacctt gtcttccgct cccgattggg tatggatcac ataccatttg cccttgagaa
     1441 tatcgatcac gggtatatcg ccttctttgc ttctatctta gctgaccagg aacttcagca
     1501 tggcggcgaa caggtagtcc atcacgccaa tgaagagacc gagaaaaatg gaagaaacga
     1561 ccaccgcttt ggtggagagc ttgacgtttt catacgccgg ccagaccacg cggcctttat
     1621 tcggtcgcac ttcgatccag acttcctgca gatagcggga gaagtctttc cacatcaatc
     1681 tcagcttttc catggggccc ccagtatgca atggtgttgc tcaacaggcc aggagggaat
     1741 cgaaccccca acccccggtt ttggagaccg gtgctctgcc aattgagcta ctggcctagt
     1801 ccattctcac ctgcgcgaga atgaaaaagg tcgcttactt gccttcttta tgaagggtgt
     1861 gcttccgttc cgccgggcaa tacttcttca tttcgatgcg gtccggatcg tttcgcttgt
     1921 tcttctgaga gctgtaattc ttgcgtttgc aggaagtgca aaccaggtta atctgttcgc
     1981 gcatattctt ttccctttcc gaaaaacggc cggcgtgtcc tggccggccg tctccatctc
     2041 gggtttacgc tcccagccgc gtgattgcgg ctgggaggaa aacacttgtt tactcgtcga
     2101 gaatcttggt caccacgccg gcgccgacag tgcggccacc ttcgcggatg gcgaagcgga
     2161 gaccggcttc gcaagcgatc ggagtgatca gttcgccaat gacttcggtg cggtcaccgg
     2221 gcatgcacat ttctttgcct tcggtcagcg tgcaggtgcc ggtcacgtcc gtggtgcgga
     2281 agtagaactg agggcgatag cccgtgaaga acggggtatg gcgaccacct tcgtccttgg
     2341 tcaggatgta gacctggccc atgaacttct tgtgaggctt gatgctcttg ggtttgcaaa
     2401 gaaccatgcc gcgctcgacg tcttctttgt tgatgccgcg cagcagcacg ccgacgttgt
     2461 cgccggcctg accggactcg aggatcttgc ggaacatttc aacgccagtg acaacgctgt
     2521 tcttgttctc ttcgcggaga ccgacgattt cgatggcatc gccgacctta accataccgc
     2581 gctcgatgcg gcccgtcacg acggtgccgc ggccggtgat ggtgaacaca tcttcgacgg
     2641 ccagcaggaa gggcttgtcc acttcgcgct cgggcagcgg aatgtattca tcgagggcct
     2701 tgaccagatt gtgaatggcc tgagggccat acttgccggc gccggcaatg gcttcggtgg
     2761 cagaaccgcg gatgaccggg gtcttgtcgc cggggaattc gtacttgctg agcaggtcgc
     2821 gaatttcgag ttcgacgaga tcgagcagct cgggatcatc gacgaggtcg cacttgttca
     2881 tgaagacgac gatgtacgga acgccgacct ggcgggcgag gaggatgtgc tcgcgggtct
     2941 gcggcatcgg accgtcagcg gcggaaacca cgagaatcgc gccatccatc tgggcgg
//
LOCUS       DAORHD010000553         2984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38247_length_2984_cov_3.291909, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000553 DAORHD010000000
VERSION     DAORHD010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38247_length_2984_cov_3.291909"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PKO06_11990"
     CDS             complement(<1..959)
                     /locus_tag="PKO06_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70411.1"
                     /translation="MRFIYRVLSSVLLFLFLSGVLAASTWQVYDTSSGLPGNKVTSFA
                     VGKKQMAVGTTQGVAVFDGETCQWSPLPLPADIASAEVRDLAYDDNEGLWMATSNGLC
                     FLPAESENTRNASRETRQANVVRYLVEDGLPNIDAGRIQVTGATVMAGFFGGYVARGT
                     VPQGGKCTFQPVNYLPARLDEGALKIRSVGVTGLAMKDSIDGWFSTQGSGLVKVSGLS
                     MRVLDQSAGLGSDWVDSFWIFPGESRSEHLLAATGEGLTMLRDEQVLGKTAFPREGVW
                     LTSLVAYLDTQDTAGRKPDPNDEPLRKFLLGRSLWVGTKSDGL"
     gene            1244..2788
                     /locus_tag="PKO06_11995"
     CDS             1244..2788
                     /locus_tag="PKO06_11995"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNV70412.1"
                     /translation="MILSVLLAGCGGGGGGGGNVVPPISEADAAQMKAAVNGFIAALQ
                     SSNAEATTTYCSQRLLTAGTLGVLTLKDLEGRTWEFTVPADGVTLLSADLAILRAFIE
                     EIEGYRIDLTFRLAREGGRWYIDDITLQETPVSVEKPAPTVTAINPATGVNTGDLANV
                     TITGTGFQSGVTVQLTRTGQNPIQAKADSTVIADDGKTITCTFPLANAAVGSWNLVIT
                     NPDGKSVTGANLFVVTSPAPSPEPLVSNVTPGSGSNNGSVNITVIGENFVTGATVKLT
                     LGQLVIQAQNVVVAADGQSLTCTLPLAQQETGEWTFTVTNPDNQSNDWGSPFLVEPPE
                     PFITSVSPSVGENDGPVNITISGVRFQTNATVTLSKSDTTLTATTVIVDGPTQIRCTL
                     PITGAQLGEYSLTVTNPDSLTTSTSFMVVPPSPPPPTISNIFADTDNITAIGSGFQAG
                     ATMRYRLQGDEEWTAATDVTILQDGNTLNGRHTQPLLQELYEVEVTNPGAEPVVEILD
                     LRVPTP"
BASE COUNT          647 a          845 c          863 g          629 t
ORIGIN      
        1 cagaggccgt cgctctttgt gccgacccag agactgcgac cgagcagaaa ctttcgcagc
       61 ggctcgtcgt tggggtcggg tttgcggccc gcggtatctt gagtgtccag gtaggcgacg
      121 aggctggtga gccagacgcc ttcgcgcgga aaggcggttt tgccgagaac ctgctcgtcg
      181 cgcaacatgg tgaggccctc gccggtagcc gcgagcaggt gttccgaacg cgattctccg
      241 gggaagatcc agaacgagtc gacccagtcg gacccaagcc cggcactctg gtcgagaacc
      301 cgcatgctga gaccggagac tttgacgaga cccgagccct gcgtcgagaa ccagccgtcg
      361 atcgagtctt tcatggccag gccggtgacg ccgacggaac ggattttgag cgcgccttca
      421 tcgagtcgtg ccggaaggta attcaccggc tgaaacgtgc atttaccgcc ctgcggaaca
      481 gtgccgcgag cgacgtagcc cccgaaaaac ccggccatga cggttgcccc cgtcacctga
      541 atgcgcccgg catcaatatt gggaagcccg tcctcgacga gataccgaac cacattcgcc
      601 tgtcgggttt cgcgtgaggc attgcgggtg ttctcgctct cagctggaag aaagcacagg
      661 ccgttcgagg tcgccatcca gagcccctcg ttgtcgtcgt aggccaggtc gcggacttcc
      721 gcggaggcga tatccgccgg caggggaagc ggcgaccatt gacaggtctc accgtcaaag
      781 acggcgaccc cctgcgtggt gccgactgcc atctgcttct tgccgaccgc gaacgaggta
      841 accttgttcc ccggcaggcc tgaactcgta tcgtacacct gccacgtcga agccgcgaga
      901 acgccggata agaagagaaa aagaaggacg gaactcagaa cgcgatagat gaaacgcata
      961 ccacctcttt tcggctacgg gtagtaaagt ataccaccga gttgctgtct gcttgtgtca
     1021 tgcactgatg ttttggcagg tttttcgcat cagcaccttc tgtctatttt gcgcatctta
     1081 tgctaccatg cctgctcgat tccgcttatc tgagaagagg aacagcaggt gagacagggc
     1141 ttttgtcgtc agccgacgtt ttgcagtctc gagaacagaa aacctgttcg agggggaagg
     1201 gaagattatg tgtaagccaa tcacgagatt catcgcagga tgtttgatac tttcagttct
     1261 gcttgctggc tgcggcggcg gagggggcgg cggcggaaat gtcgtaccac cgatctcgga
     1321 agccgatgcg gcccagatga aagcagccgt gaacggtttc atcgccgctc tgcagagttc
     1381 gaatgccgag gcaacgacta cctattgctc acaacgtctg ctgacagctg gtactcttgg
     1441 cgtgctgacc ttgaaagatc tggaaggcag gacctgggag ttcaccgttc cggctgatgg
     1501 cgtgacactg ctttcggctg atcttgcgat actcagggct ttcatcgaag aaatcgaggg
     1561 ctatcgcatc gatttgacgt tccggctcgc aagagaaggt ggccgctggt acatcgacga
     1621 cattaccctt caggaaaccc cggtttcggt cgaaaaacct gccccgaccg tcaccgcgat
     1681 caatccggcg accggagtga acaccggtga tctcgcgaat gtgacgatca cgggaaccgg
     1741 ctttcagagt ggcgttacgg ttcagttgac tcgtacggga caaaacccga ttcaggcaaa
     1801 ggcagacagc accgtcatcg ctgatgatgg aaagacgatc acctgtacct tcccccttgc
     1861 caacgccgcc gtcggatcct ggaacctggt catcaccaac ccggacggta agtctgttac
     1921 cggagcgaat ctgtttgtcg tcacttcccc tgctccgtcc ccggagcccc ttgtatcaaa
     1981 tgtaacgcca ggctcaggat caaacaacgg atcggtgaac atcaccgtca ttggcgagaa
     2041 tttcgtgaca ggagcaaccg tgaaattgac tctcggacaa ttggtgatcc aagcgcaaaa
     2101 cgtcgtggtt gctgccgacg ggcaaagtct gacttgcact ttgcctctcg ctcagcagga
     2161 aacaggtgag tggacgttta cggtcaccaa cccggacaat caatcgaatg attggggctc
     2221 gcctttcctc gtcgaaccac cggaaccttt catcacttca gtgtctccga gcgttggcga
     2281 gaatgatggt ccagtcaata tcaccatcag cggtgtccgg tttcaaacca acgctaccgt
     2341 cactctgtca aaatctgaca cgacattgac agcgactaca gtcattgtcg acggtccgac
     2401 gcagatcaga tgcactcttc cgattaccgg tgctcagctt ggggagtata gtctcaccgt
     2461 taccaacccg gacagtctga cgacatcgac ttcattcatg gtggtgccgc cttcgcctcc
     2521 tcctccgacg attagcaaca tattcgctga caccgataac atcacggcca tcggttcagg
     2581 cttccaagcc ggtgccacca tgcggtatcg ccttcagggc gatgaagagt ggaccgccgc
     2641 tacggatgta accattttgc aggatggcaa cacactgaac ggacgtcaca cgcaaccgct
     2701 gttgcaggag ttgtacgagg ttgaagtcac caatcccggg gcagaaccgg tcgtcgagat
     2761 tctcgatctc cgcgttccga ccccgtgagt ttttgggacc tgagaagctt ttgcggcaac
     2821 gaagcgcctg gtgaccggcg catttgccgg aggatagaag gagaatgata tgcgaagtgt
     2881 aatctgccga atcttggcgg tgctcgtggt tttgagtccg gtgtgggctc atgccgctga
     2941 tctggttgac agcggggtgc agttgcgaga tggaagtggg gttg
//
LOCUS       DAORHD010000554         2980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38333_length_2980_cov_2.801709, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000554 DAORHD010000000
VERSION     DAORHD010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38333_length_2980_cov_2.801709"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /gene="coaD"
                     /locus_tag="PKO06_12000"
     CDS             complement(<1..436)
                     /gene="coaD"
                     /locus_tag="PKO06_12000"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV70413.1"
                     /translation="MRIAVYPGSFDPFTLGHLDILERSMKMFDKVIVGVLVNSSKKCL
                     LQPSDRVALINEVLKGRPQVEVREFEGLLVDFCWKMEACAIIRGLRAVSDYEYEMQLA
                     TINKQLAPTLETVFLMSSSQYSFLSSSIVKEIARFGGDIASLV"
     gene            complement(433..1065)
                     /locus_tag="PKO06_12005"
     CDS             complement(433..1065)
                     /locus_tag="PKO06_12005"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNV70414.1"
                     /translation="MKSSSQTLPNLYLASQSPRRRQILEELTVPFQVISSPYQENAEE
                     FAHLPPAEQAAKLASLKAFHAARNLSEGLVVGADTIVVQEERVLGKPKDRDDARQMLQ
                     SLSGRPHQVITGLAIVNVDGLVTRSRAETTRVFFRKLTDADIVFYINTREPYDKAGAY
                     AIQGHAGLFVERIEGCYFNVVGFPVVAFANLMKETGYDLLEYIRRSGAAS"
     gene            complement(1062..1580)
                     /locus_tag="PKO06_12010"
     CDS             complement(1062..1580)
                     /locus_tag="PKO06_12010"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNV70415.1"
                     /translation="MYNHNPARVQGLRAWTLLAVLSAAAAALQVIESPLPRLLPWLKP
                     GLANSLTLYALVRWTPGFCVALVVLRTLIAGLALGMLLSPANLLSLAGGLAGALAMWA
                     GLLCGRDRLSLYGISIIGAAANNLAQLLTVQWMFASGFPLWFHLSVMLWVSIPSGMIV
                     GHISRELLRRTA"
     gene            complement(1616..2005)
                     /locus_tag="PKO06_12015"
     CDS             complement(1616..2005)
                     /locus_tag="PKO06_12015"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNV70416.1"
                     /translation="MMTAEESLARPLRGDLFLVLLLAVVGALLAHWNGANGTNSWQVQ
                     IVGRPEKTLVLDGLVAATYDTGGWLGTATIECDGANRWRFVRSPCPNQVCVRSGWFTS
                     PVGVPCVPNGIIVEPRHQPAGVDGVSR"
     gene            complement(2002..>2980)
                     /locus_tag="PKO06_12020"
     CDS             complement(2002..>2980)
                     /locus_tag="PKO06_12020"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV70417.1"
                     /translation="ILLLVVVLVLGALAGRESATGPTVLRTESMMDTLITIKAWGPEE
                     VSKVAVDRGFEVFRRLDRLASFHREDSELSQFNRHRTLAATASLSVLLDIAARSYQQT
                     TGVFDPTFAVLHRAYGFYDQRGRIPSEQQLNEILAFVGWDKMVSRRDDGWHLASGALV
                     DLGGVAGGYAVDQAAAIMRESGCTAFLIDDAGDLWMEGTKPGSEPWKIMVRDPRGEQA
                     LAFVESAVPVAISTSGDYERFVEVDGRRICHIFVPSTGKSADWYRSVTVVASSSLAAD
                     TLSTTLFAMPPGQAREWAEAHGVAALFLPATGSTWLSAAGTAWFTHVVP"
BASE COUNT          665 a          955 c          846 g          514 t
ORIGIN      
        1 ggacaaggct ggcgatatcc ccgccaaaac gcgcgatttc tttcacgatg ctcgaactga
       61 gaaacgagta ttgtgacgac gacatcagga acaccgtctc gagcgtgggc gcgagctgct
      121 tattgatggt ggccagctgc atttcatatt cgtaatcaga aaccgcacgt agcccacgga
      181 tgatcgcaca cgcttccatc ttccagcaga agtcgaccag caacccttcg aattcgcgca
      241 cctcgacctg agggcgcccc ttcagaacct cgttgatcaa cgccacccgg tctgatggct
      301 gcaacagaca ctttttgctt gagttcacca ggacgcccac gatgaccttg tcgaacatct
      361 tcatggaccg ttcgagaata tcgagatgcc ccagcgtgaa cgggtcaaaa cttcccggat
      421 acaccgcgat tctcatgatg cagctcctga acggcgaatg tattcaagca gatcataccc
      481 ggtctctttc atcaggttgg cgaaagccac gaccgggaac ccgaccacgt tgaagtaaca
      541 cccctcgatg cgctcgacaa acagcccggc atgcccttga atcgcgtaag ccccggcctt
      601 gtcatacggt tcccgggtat tgatgtagaa cacgatatcc gcatcggtga gtttccggaa
      661 gaacacgcgc gtggtctcgg cccgggagcg ggtgaccagg ccatcgacgt tcacgatcgc
      721 caggccggtg atgacctgat gcggacgccc tgacagcgac tgcagcatct gccgtgcgtc
      781 atcgcgatct ttcggcttgc ccaggacgcg ctcctcctgc accacgatcg tatcggctcc
      841 gaccaccaga ccttcgctca ggttgcgcgc ggcgtgaaac gccttgagtg acgccagctt
      901 ggcggcctgt tccgcgggcg gcaaatgagc gaactcttca gcgttttcct gatacgggga
      961 ggaaatcacc tgaaatggca ccgtcagttc ttcgagaatc tgacgtcggc gtggcgactg
     1021 cgaagcgaga tacaggttgg gaagtgtctg agaagaggac ttcatgcggt tctcctgaga
     1081 agttcgcgcg agatgtgccc gacaatcatg cccgagggaa tactgaccca cagcatgacg
     1141 ctgagatgaa accacaacgg gaatccggat gcaaacatcc attgcacggt cagcaactgc
     1201 gccaggttat tcgcggccgc gccaatgatg ctgataccgt agagactgag tcgatcacga
     1261 ccgcagagca ggcccgccca catggccagg gccccagcca agccacccgc cagcgagagc
     1321 agattcgccg gtgacagcag catgcccaga gccagaccgg caatcagcgt gcgcaagacc
     1381 acgagagcca cgcagaaccc cggggtccat cgtaccagcg catacagcgt cagactgttt
     1441 gccagacctg gtttcagcca tggcaacagg cgaggaagcg gcgattcgat cacctgcaag
     1501 gcggccgcgg cggccgacag caccgccaga agcgtccagg ctctcaaacc ctgaactcgc
     1561 gccgggttat ggttatacac tctgtctatc tggggagaag aaacggaggg tgggttcatc
     1621 gcgagacccc atctacgcct gctggctggt gacgtggctc gacgatgata ccgttcggca
     1681 cgcagggcac accgacgggc gaggtaaacc accccgagcg cacgcacacc tgattcggac
     1741 acggcgagcg gacaaagcgc cagcgattcg cgccatcaca ctcgatcgtc gcggtaccga
     1801 gccacccgcc cgtgtcgtag gtcgcggcaa ccaacccgtc gagaaccagg gtcttctcag
     1861 gtcgcccgac gatctgcacc tgccaggagt tcgttccatt cgccccgttc caatgagcga
     1921 gcagcgctcc gacgaccgcg agaagcaaca cgagaaagag gtctccccga agcggtcgag
     1981 ccagggactc ttctgcggtc atcatgggac gacgtgcgtg aaccaggcgg ttccggcagc
     2041 ggagagccag gtcgaacccg tcgccggcag aaacagcgcg gccacaccgt gcgcctcggc
     2101 ccactcgcgc gcctgcccgg gcggcatggc aaacagcgtg gtgctcaacg tatcggctgc
     2161 cagcgaggag ctggcgacca ccgtgaccga tcggtaccag tccgcgcttt tgcccgtgct
     2221 gggcacgaaa atgtgacaga tgcgtctccc atcgacctcg acaaaccttt cgtagtctcc
     2281 gcttgtcgaa atcgcaaccg gtacagcact ctcaacgaag gccagcgcct gctcaccacg
     2341 cggatcgcgc accatgattt tccagggctc actgcccggc ttggtcccct ccatccacag
     2401 atcaccggca tcatcaatga gaaaggcggt acacccgctc tcgcgcatga tcgccgccgc
     2461 ctgatcgacc gcgtagcctc ccgccacacc tccgagatcg accagggctc cggaagcaag
     2521 atgccagccg tcatcgcgac gcgagaccat tttgtcccag ccgacgaaag cgagtatttc
     2581 gttcagctgc tgttctgagg gaatacgacc gcgttgatcg tagaacccat acgcgcgatg
     2641 cagcacggcg aaggtcggat caaacacccc ggtggtctgc tgatacgatc gagcggcgat
     2701 atccagcagc accgataacg aagccgttgc agccagggtt cgatggcgat tgaactggga
     2761 caattcggag tcttcacgat gaaacgaggc cagacgatca aggcgacgaa atacttcgaa
     2821 ccctcggtcc accgcgactt tcgagacctc ttcggggccc caggccttga tggtgatcaa
     2881 ggtatccatc atcgattctg tacgcagaac cgttggcccg gtagccgact cgcgtccggc
     2941 gagtgcaccc agcacgagca ccaccaccag gagcaggatc
//
LOCUS       DAORHD010000555         2975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38415_length_2975_cov_3.829795, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000555 DAORHD010000000
VERSION     DAORHD010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38415_length_2975_cov_3.829795"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PKO06_12025"
     CDS             <1..237
                     /locus_tag="PKO06_12025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014380498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNV70418.1"
                     /translation="LRPEVILNAAALTHTSIGLRDVLTYAKVPVVEVHITNITAREEF
                     RRTSLLTPVCRGIISGLGTSGYLLALRYLAGKVE"
     gene            263..2521
                     /locus_tag="PKO06_12030"
     CDS             263..2521
                     /locus_tag="PKO06_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HNV70419.1"
                     /translation="MGGTTRPELLAPAGDRECLKAALVAGADAVYLGGKHFGARAFAA
                     NFDEAGLRWARRVTRSLGRKLYLTLNTLIFDDEWPLLEKWLDFCETLQPDALIIQDLG
                     LAATLERRHSQIPRHLSTQGAWDGFGGADVLRHLGISRVILPRETDLSRLRELVQSSP
                     FELEVFVHGAMCYSVSGRCFWSVALGQRSGNRGTCAQPCRKLYVAERGRTNTFFSPRD
                     LRLAPHLSDLAGSGVASLKIEGRMKDAAYVYQVVRVYRQLLDGHPHTEELISELDHVF
                     TRPSHHGFISGTPVANWYTQDDGGRGFAEIGRVSGGRRADGLLEIECRREVRPGDGLA
                     WAIGGRREGARLTWVAALTQKKQHYLVRGLPSLPLDTPLLLSDTATARPWESEWNKEW
                     EREPIDLFWSGHDEQPLAVEFTYKGQAARLTSPGNLARARGQGMEDGPLQERFANLGE
                     HFRAGRHVIRALDRGLFLNPKDLKALKRELVDHLQKMETLYAPAAKSPSMLRPSNGAS
                     SVPADESESRHAKPEVEQGKRVRLAVRLWSAQAITFRRFQPDVWILPLTNDPLPISLQ
                     EAPVSWWIPPPTSATELRQLIDRLRPLESQDFLCLGWEAFTLARELPHHRFRLDWLFN
                     LVNPTAMRVVQKNGVRVTAAREWPARLPIPEECWRTVAWNPLLSISRFPPSVSLGALF
                     ANPHGDRFFLDQIGPGLFGLFLEQRPVIPPPGSRGWVQVDVAFPAHEKPLKRVGELEL
                     FLQTLRREAEGT"
     gene            complement(2569..>2975)
                     /locus_tag="PKO06_12035"
     CDS             complement(2569..>2975)
                     /locus_tag="PKO06_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV70420.1"
                     /translation="SKLALTYAASFSMEFGAEVHLLHVIEEETVHVGSLEDPRHIGEK
                     WEKESLEKLGEFVDARFKELPIVMKATGGEITDTIFAYAQENNVDLIIMGARGQTGFV
                     STLLGSTTYDVARKAPCPVLTVKPYDHPFITS"
BASE COUNT          548 a          829 c          962 g          636 t
ORIGIN      
        1 ctgcgtcccg aggtgattct caacgcggcg gccctgacgc ataccagtat cggcctgcgc
       61 gatgtgctga cgtatgccaa ggtgccggtg gtggaagtgc atatcaccaa catcacggcc
      121 cgtgaggagt ttcgtcgcac gtcgttgttg acaccggtct gccgcgggat catctcgggt
      181 ctgggtacca gcgggtatct gctggctctg cggtatctgg cgggaaaagt cgagtagtgt
      241 cggttgcggt atgagtggag ccatgggtgg aacaacgcgg cctgaactgc tggcccccgc
      301 gggagatcgg gagtgcctga aagcagcgct ggtcgccggc gctgacgccg tgtacctcgg
      361 cggcaagcat tttggggcgc gggcgtttgc cgcgaacttt gatgaagcag gactgcgctg
      421 ggcgcgtcgc gtcacccggt ctctcgggcg taaactgtat ctcaccctga atacgttgat
      481 cttcgacgat gaatggccgc ttctcgaaaa gtggctcgat ttttgtgaaa ccctgcaacc
      541 cgacgcgttg atcattcagg atctcgggtt ggccgcgact ctcgaacgac gccacagtca
      601 gattccgcgg catctcagca cgcagggagc ctgggacggg tttggtggtg cggatgttct
      661 gcgccatctg ggaatttcgc gagtcattct tccgagagaa acagaccttt ctcgtttgcg
      721 cgagctggta caaagcagcc ccttcgaact cgaagtattc gtgcacggcg cgatgtgtta
      781 cagcgtgtcg ggacgttgtt tctggagtgt ggctctgggc cagcgttctg gaaaccgtgg
      841 cacctgcgcg cagccctgtc gtaagctgta tgtcgcggag cgcggtcgca ccaacacttt
      901 tttcagtcca cgcgatctgc gtctggcgcc gcatctgtcc gatctggccg ggagcggtgt
      961 ggcgtcgctg aagatcgagg ggcgcatgaa agatgccgca tacgtgtatc aggtggtgcg
     1021 ggtctatcgc cagttgctcg atgggcaccc gcacaccgaa gaactgattt ctgagctcga
     1081 tcacgtattt accagaccat ctcatcatgg attcatcagc ggaacacccg tcgctaactg
     1141 gtacactcag gatgacggag gacgcggctt cgcggagatc gggagagttt cggggggacg
     1201 gcgtgccgac ggcctgctgg aaatcgagtg tcgtcgcgag gttcgtccgg gcgatggcct
     1261 ggcctgggcc attggaggtc gtcgggaagg ggcgcgactc acctgggtcg ctgcgctgac
     1321 acagaagaaa cagcactatc tggtgcgcgg tctgccttct ctgccgctcg acaccccgtt
     1381 actgctcagt gacacggcga cggctcgacc ctgggaaagt gaatggaaca aggaatggga
     1441 gcgtgagccg atcgatctct tctggtcagg acatgacgag cagccattgg ccgtcgaatt
     1501 cacgtacaaa ggccaggcgg cacgtttaac cagcccggga aatctggcgc gggcgcgcgg
     1561 gcagggcatg gaggacggac cactgcagga acgctttgcc aatctgggtg agcactttcg
     1621 ggccggccgc catgtcatac gggcactcga tcgcggtctc tttctgaacc cgaaagattt
     1681 gaaagcgctg aagcgggaac tggttgatca tctgcagaag atggagacac tctacgcacc
     1741 ggcggcgaag tctccgtcga tgctgcggcc atcgaacggc gccagttccg taccggctga
     1801 tgagagcgaa tcgcggcatg cgaagcccga agtcgaacag ggcaaacggg tgcgtctggc
     1861 cgtgcggctc tggtcagccc aggcgatcac gtttcggcga tttcagcccg atgtctggat
     1921 tctgccgttg acgaacgatc cgttgccgat cagtctgcag gaagctcctg tgtcctggtg
     1981 gattccgcct ccgacttccg ccactgaact gcggcaactg attgatcgac tgcgcccgct
     2041 ggaatcgcag gactttctct gccttgggtg ggaggcgttc accctggcac gtgagctgcc
     2101 acatcatcga tttcgtcttg attggctgtt caacctggtg aatccaaccg ccatgcgggt
     2161 ggttcaaaaa aacggagtgc gggtgacggc agcccgggag tggccggcgc gtctgccgat
     2221 cccggaagag tgctggcgaa ccgtggcgtg gaatccgctc ctctcgatca gtcggttccc
     2281 gccgtctgtc tctcttggtg cgttgtttgc taatccgcat ggcgaccgtt tttttctcga
     2341 tcagatcggc ccggggttgt tcggcctgtt tctcgaacaa cggccggtga ttccgccgcc
     2401 ggggtcacgc ggatgggtac aggtcgatgt ggccttcccg gctcatgaaa agccgttgaa
     2461 aagggtgggg gaactcgagt tgtttttaca gacgctgcgc cgcgaggcgg aaggaacctg
     2521 aaagaaaacg cccccggtct cggaagaccg ggggcggagt gtctgaggtc acgaggtaat
     2581 gaaggggtgg tcgtagggtt tgacggtgag aacggggcat ggagccttgc gagccacgtc
     2641 gtaggtcgta ctccccagca gggtgctcac aaaaccggtc tgtccgcgtg cccccatgat
     2701 gatcaggtcg acattgtttt cctgggcata ggcaaagatc gtatcggtga tctcgccgcc
     2761 ggtggccttc atcacgatcg ggagttcctt gaagcgggcg tccacgaact cgcccagttt
     2821 ctccagactt tccttttccc acttttcccc gatgtgacgc gggtcttcaa gactgccaac
     2881 atggacggtt tcttcctcga tcacatgcag caggtgcact tctgcgccga attccatcga
     2941 gaaactggcg gcataggtca gagccagctt cgaac
//
LOCUS       DAORHD010000556         2974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38430_length_2974_cov_4.483385, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000556 DAORHD010000000
VERSION     DAORHD010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38430_length_2974_cov_4.483385"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKO06_12040"
     CDS             complement(<1..697)
                     /locus_tag="PKO06_12040"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV70421.1"
                     /translation="MIRRVWMIVWKEFLQIGRDPRMLGVTVILPIFMVVLYGYAINLD
                     VRHLKLAILDEDRTGASRELLAAMTHNEYFLPVRDLSHRREIEPILLSGEAKVVIVIP
                     RGYAESLAANQATDIQLLIDGTDSTSANTGIGYVNAIIREQSARLTLAAVRRVGSIRP
                     DGVVPIENRFRNWYNPELRSANFIIPGLIAVILMMLSALLTSVTVVRERERGTIEQLI
                     VSPLTAFELMVGKL"
     gene            complement(694..1692)
                     /locus_tag="PKO06_12045"
     CDS             complement(694..1692)
                     /locus_tag="PKO06_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV70422.1"
                     /translation="MTSVSSPVSPRTRPTTVEVDRLTRTFGSFTAVDQISFAVNQGEV
                     FGFLGPNGAGKSTTIRMLCGLLLPTAGTGRVGGFDVHTESERIKENIGYMSQRFSLYE
                     DLTIDENIRFYADIYNVPYERLAARIDWVYDLTHLSDRRHRLTGVLPLGLKQRLALGC
                     AVIHEPPILFLDEPTSGVDPISRRSFWELIYQLAHQGTTVFVTTHYMDEAEHCDRLAM
                     IYRGRLVAIGTPEEMRATAIGGSLLRIEADPLMDALEVLEHEPSVLDVAVFGASLHVR
                     VTDPASAAHAVKQRLANTGIRLETLEPVRPSLEDVFVSLVEQADDAAQPASEAVNP"
     gene            complement(1689..2660)
                     /locus_tag="PKO06_12050"
     CDS             complement(1689..2660)
                     /locus_tag="PKO06_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV70423.1"
                     /translation="MNSSPGMAEPDNGLAVTRLCRHFGDVQAVADVSFEVRPGEIFGI
                     VGPDGAGKTTLFRLLCGALDIDSGEARVGPFDVRRDPESVKSIIGYMSQRFSLYGDLT
                     VAENIHFTATLFHVSAADRQERERQLLAASRMEPFRHRLARDLSGGMKQKLALTCALI
                     HRPRILFLDEPTTGVDPVSRRDFWKILYQLVRQGMTLVVSTPYMDEAERCNRVAFMNA
                     GRLLCCASPRELKANGHGSLVEVDVRPVIPARHVVAALPFVQSVHVFGNRLHVRLAEP
                     GEPARHLADALSRSGLDYRDQRVISPSLEDVFISLVTEPVSPAPQNA"
     gene            complement(2657..>2974)
                     /locus_tag="PKO06_12055"
     CDS             complement(2657..>2974)
                     /locus_tag="PKO06_12055"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV70424.1"
                     /translation="GLPVLQIADLANVWLKVYVAETQVGLIKPGQLADVTVDSFAGKI
                     FKGRVSEIAEIPEFTPKNVQTRDERTKLVFWVKIAIENPDLELKPGMPGDAVIHIDTP
                     TRP"
BASE COUNT          604 a          816 c          987 g          567 t
ORIGIN      
        1 tcagtttgcc gaccatcagt tcgaaagcgg tcaggggcga gacgatgagc tgctcgatcg
       61 tgccccgttc gcgctcacgt acgacggtga cggatgtcag caaggccgag agcatcatca
      121 gaatgacggc gatcaagcct gggatgatga agttggcgct gcgcagttcg ggattgtacc
      181 agttgcgaaa ccggttctcg atcgggacga cgccgtcagg gcgaatactg ccgacacgac
      241 gcacggctgc cagggtcaga cgggccgact gttcgcggat gatggcattc acgtagccga
      301 tgccggtgtt ggcactggta ctgtcggtac catcgatcag cagctggata tcggttgcct
      361 ggttggcggc gaggctttcc gcgtagccgc gcggaatcac gatgaccacc ttcgcctcac
      421 ctgacagcag aatcggctcg atctcgcgac gatgactcag gtcgcggact ggcagaaaat
      481 actcgttgtg ggtcatggca gccaggagtt cgcgtgaagc gccggtgcgg tcttcatcga
      541 gaatggcgag cttcagatgg cgtacgtcga gattgatggc gtagccatac agcaccacca
      601 tgaagatcgg caggatgacc gtgacgccga gcatgcgcgg gtctcgaccg atctgcagaa
      661 actccttcca gacgatcatc cagacacggc gaatcatggg ttgaccgcct cactggccgg
      721 ttgggccgcg tcgtctgcct gctcgaccag gctgacgaag acgtcttcca gcgagggacg
      781 caccggttcg agggtttcga gacgaatgcc ggtgttcgca agccgttgct tgaccgcatg
      841 ggcggctgag gcgggatccg tcacacgtac gtgcagtgat gctccaaaaa cggcgacatc
      901 gagcacggaa ggttcgtgtt ccaagacttc gagggcgtcc atcaatgggt ccgcttcgat
      961 gcggaggagt gaaccgccga tggccgtggc gcgcatctct tccggagtac cgatggcgac
     1021 cagacgaccc cgatagatca tggccagtcg atcgcagtgt tccgcctcgt ccatgtagtg
     1081 ggtcgtcacg aacacggtcg tgccctgatg ggccagttgg tagatgagtt cccagaagct
     1141 gcggcgcgag atcgggtcga cacctgatgt cggttcatcg agaaacagaa tcggaggttc
     1201 atgaatgacg gcgcagccga gagcgagccg ctgtttcagt ccgagtggca gtacgccggt
     1261 cagtcgatgg cgtctgtctg agaggtgggt taaatcatag acccagtcga tacgagccgc
     1321 gagtcgctca tatggaacgt tatagatatc ggcgtagaag cgaatgttct catcgatggt
     1381 cagatcttcg tagagcgaaa accgctggct catataccca atgttttctt tgatgcgttc
     1441 tgattcggtg tgcacatcaa agcctccgac gcggccggta cctgctgtcg gcagaagcaa
     1501 gccgcacagc atgcgaatgg tcgtggactt ccccgcgccg ttcggcccga gaaagccgaa
     1561 gacctcgccc tggttcacgg cgaaactgat ctggtcgacc gcggtgaaac tgccgaacgt
     1621 tcgcgtcagg cgatcgactt cgaccgtggt cggacgagta cggggagata caggagaaga
     1681 aacgctggtc atgcgttctg tggtgccggg gaaacaggtt cggtgacgag tgagatgaac
     1741 acgtcttcga gactgggaga gatcacgcgc tgatcgcggt agtccaggcc agatcgagag
     1801 agggcgtcgg cgaggtgccg ggccggctca cccggctcgg cgaggcgaac gtgcagacga
     1861 ttgccgaaga catggacact ctgaacgaat ggcagggccg caaccacgtg tcgagccgga
     1921 atcaccggcc gaacatcgac ttcgaccagg ctgccgtgac cgttcgcctt gagttcgcgc
     1981 ggggaagcgc agcagagcag acgtccggcg ttcatgaacg cgacccggtt gcagcgttcc
     2041 gcttcgtcca tatacggggt cgaaacgacg agtgtcatgc cctgacgaac gagctgatac
     2101 aggattttcc agaagtcgcg gcgcgagacc gggtcgactc cggtggtcgg ttcatcgagg
     2161 aacagaatgc ggggccgatg gatcagggcg caggtgaggg cgagcttctg cttcatgccg
     2221 cctgaaagat cgcgggcgag ccgatggcga aatggttcca tgcggctggc ggccagaagc
     2281 tggcgttcgc gttcctgtcg gtcggcggcg gaaacgtgaa acagggtggc ggtgaagtga
     2341 atgttctcgg cgaccgtgag atcgccgtac agactgaagc gctgggacat atacccgatg
     2401 atgctcttga ccgattcggg atcacggcga acgtcgaacg gtccgactcg ggcttctcct
     2461 gagtcgatat cgagcgcgcc gcagagcaac cggaagagag tggtcttgcc cgcgccgtct
     2521 ggcccgacaa tgccgaaaat ttccccgggg cggacctcga aactgacgtc ggcaaccgcc
     2581 tgtacgtctc cgaagtggcg gcagagtctg gtgacggcaa gcccgttgtc gggttcagcc
     2641 ataccgggag aggaattcat gggcgggtcg gtgtgtcgat atggatcacg gcgtcgcccg
     2701 gcatgccggg tttcagctcg agatcaggat tttcgatggc gatcttcacc cagaagacga
     2761 gcttggtgcg ttcgtcgcgg gtctggacat tcttcggcgt gaattcggga atctcggcta
     2821 tctcgctgac acgccccttg aagatcttcc cggcgaagga gtcgacggtg acgtccgcga
     2881 gttggcccgg tttgatcagg ccgacctggg tttcggcgac gtagaccttc agccagacgt
     2941 tggccagatc ggcaatctgc agcaccggca gacc
//
LOCUS       DAORHD010000557         2971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38491_length_2971_cov_3.747257, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000557 DAORHD010000000
VERSION     DAORHD010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38491_length_2971_cov_3.747257"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..1296)
                     /locus_tag="PKO06_12060"
     CDS             complement(130..1296)
                     /locus_tag="PKO06_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70425.1"
                     /translation="MSFYQHTQDLLGKIAEKYRLPRLAEVIKDRDSIVWTKDVTLCVT
                     NILKALDAGGDAKALLKDAKNMYNTPIFGINPREGKFLINALPDPLKAVDEMGKYDFC
                     FKLSPYHIINTFLTVLTAQVTGYDPSVLVRKGEEHLEAFIAHSLKDNPNWKKDQLTSK
                     KPLLIIPIGAAGCGKSTFFRELGNVVNASCDNIRYLLFSDLGPCFAPWESTLAWWVVN
                     RLTDIYLGRGYSVFYNGVNTDPEYRSPITMENPHALFAGMPYTFKLVYFEPPVGLSEK
                     ELGELKNINLWATPLEKVDRASLSANVKSVLDLITRNFERTMERTQAIRDGKASQDPF
                     DILYSVPPAIVKLFVEQSFSKPTGANVVVVPRREIPDAAQRAAFYREYATKTLN"
     gene            complement(1396..2757)
                     /locus_tag="PKO06_12065"
     CDS             complement(1396..2757)
                     /locus_tag="PKO06_12065"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNV70426.1"
                     /translation="MKNRSLRAQLSIVIVLSLLWMGLCGVADGQTPGTTFVYKTVKEY
                     GPLIDLKNKLRPGEQIHESEREGFGYIVRAVPITSEQHLMQLLGAPGEDRLSDTQKGI
                     LEVYRLGQTQEIGDIQRNAPAINRAVTVNLVDITGCEDRQKFPGLRNDFWPRASKQTS
                     YSGNRSVRDIDILISGVDSQGYGARTAAEMKATFVHEIAHTLDLGQIEFGAYGSDGDH
                     WFNEVTTEKAAFCEGFANYIVTLLYPSRARDFRGSLQQIRIENASGTYQYISPSDPRL
                     KASDLLKVEGVVTLTLHQITLSIPNGRDKVLAAFRESNSWDNGLGKFLQTLIRLNPSD
                     AVAIVKVVDDETFKRLSNAEIRALFGHSALIEPYLQTRSDPAGQRQVRPGTATPAVGP
                     GVTTPPPAGGRARIYKWKDKDGRLHFTDSPPPPGVEYTVMSPQKISPLQLDGGKSNPY
                     QDD"
     gene            complement(2772..>2971)
                     /locus_tag="PKO06_12070"
     CDS             complement(2772..>2971)
                     /locus_tag="PKO06_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70427.1"
                     /translation="PDDLGTWIKLGVNYLPDHPAVLDAVSNVASFPDHGERQRMIDVA
                     KKNAVSKKPYADACRLFLDSR"
BASE COUNT          566 a          833 c          887 g          685 t
ORIGIN      
        1 cgcgcggggc gttgccatac atggcaccgc catccagtcg ttgacgattt gcttcgagtg
       61 cccacagttt catgtcccag ttcccttcag gtcaagtgca gatgcggtga gctttgtctg
      121 atgaatgatt cagttcagcg ttttggttgc atactcgcgg tagaaggcgg cgcgctgagc
      181 ggcgtccgga atctcgcggc gcggcaccac cacgacgttc gcgcccgtcg gcttcgagaa
      241 cgactgttcc acgaacagct tcacgatggc gggcggaacc gagtagagaa tgtcgaacgg
      301 gtcctggctg gctttcccgt cacgaatcgc ctgggtgcgt tccatggtgc gctcgaagtt
      361 gcgggtgatc agatcaagca cgctcttcac gttcgcgctg aggctcgcgc gatcgacctt
      421 ctcgagcggg gtggcccaga ggttgatgtt cttgagctcg ccaagctctt tctcgctgag
      481 gccgaccggc ggctcgaagt agacgagttt gaaggtgtag ggcatgccgg cgaaaagcgc
      541 gtgggggttt tccatagtga tgggcgaccg gtactcgggg tcggtgttga cgccgttgta
      601 gaacacgctg tacccgcggc ccagatagat gtcggtcagg cgattgacca cccaccaggc
      661 gagggtgctt tcccatgggg cgaagcacgg cccgagatca ctgaacagca ggtaacggat
      721 gttgtcgcat gacgcgttca ccacgttgcc cagttcacgg aagaacgtcg acttgccgca
      781 cccggccgcg ccgatcggaa tgatcaacag cggctttttc gaggtgagct gatctttctt
      841 ccagttcggg ttgtctttca atgagtgtgc aatgaaggcc tcgagatgtt cttcgccttt
      901 acgaaccagg acggaaggat catacccggt cacctgcgcg gtgagcacgg tcagaaatgt
      961 gttgatgatg tgatacgggc tgagtttgaa gcagaagtca tacttcccca tctcgtcgac
     1021 ggctttgagc ggatcgggga gggcgttgat caggaacttg ccttcgcgcg ggttgatgcc
     1081 gaagatcggg gtgttgtaca tgttcttcgc gtctttcagc agtgctttcg catcgccgcc
     1141 cgcatcgaga gccttgagaa tgttcgtgac gcagagcgtg acgtcctttg tccagacgat
     1201 ggaatcgcgg tccttgatga cctcggccaa ccgcggcagc cgatactttt cggcgatctt
     1261 tccaagcaga tcctgcgtat gctgatagaa gctcatgtcg gtacccccgc gtcagatttg
     1321 ttgtaaccct ggtacagtta ccacatttcg cggcgttttt cctctcgatc ctgagtttcc
     1381 gaaggaaacg gcactctaat cgtcttgata ggggttgctc ttcccgccat cgagctgcag
     1441 aggggagatt ttctggggac tcatgaccgt gtattcgact cccggtggcg gtggcgagtc
     1501 cgtgaagtgc aaccgcccat ccttgtcctt ccacttgtaa atgcgtgccc gaccgccggc
     1561 tggcggtggc gtggtcacgc cggggccgac ggccggtgtg gcggtcccgg gtcgaacctg
     1621 tcgttgtccc gctgggtcgg aacgggtctg cagatacggc tcgatgagcg ccgagtgtcc
     1681 gaacaaagcg cgaatctccg cattggaaag gcgcttgaag gtttcgtcat caaccacctt
     1741 cacgatcgcc accgcatcgg agggattcaa tctgatcaag gtctgcagga acttgccgag
     1801 tccgttgtcc caggagttgc tttcacgaaa cgcggccaat accttgtcac ggccattggg
     1861 aatcgacagc gtgatctgat ggagcgtcaa agtgacaact ccttcgactt tcagcagatc
     1921 gctcgctttc aaccgcggat cgctgggaga gatatattga tacgttcctg aagcgttctc
     1981 gatacgaatc tgctggagag aaccgcgaaa atcacgagcc cgactcggat acagcaaagt
     2041 cacgatatag ttggcgaacc cctcgcagaa cgccgccttt tccgtggtga cttcgttgaa
     2101 ccagtggtca ccgtctgaac cgtaggctcc gaactcgatc tgccccagat caagggtgtg
     2161 cgcgatctcg tgaacgaagg tcgctttcat ttcagcggcg gttcgcgccc catacccttg
     2221 gctgtcgacg ccactgatca ggatgtcgat gtcccgtacg cttcgatttc cggaatagga
     2281 tgtctgcttg ctggctcgag gccagaagtc gttgcgcagg cccggaaact tctgacggtc
     2341 ctcgcagccg gtgatgtcga cgagattcac cgtgacggca cgattgatcg ccggcgcatt
     2401 cctctgaatg tcgccgattt cctgagtttg tccgagtcgg tagacctcga gaatgccttt
     2461 ttgcgtgtct gacagcctgt cctctcccgg ggcgcccaga agctgcatca gatgctgctc
     2521 gctggtgatc ggaaccgcgc gcacgatgta cccgaatccc tcccgttcac tctcatgaat
     2581 ctgctcaccc gggcggagct tgtttttgag atcgatgagc ggcccatact ctttcacggt
     2641 tttgtacacg aaggttgttc ccggggtttg cccgtcagcg actccgcaaa gtcccatcca
     2701 cagaagcgac aggacaatga cgatcgatag ctgtgcgcgc agggatcggt tcttcatgat
     2761 ggcaggctcc ttcaccggga atccagaaac agacgacagg catcagcata aggcttcttc
     2821 gagacggcat tcttttttgc gacgtcgatc atccgctgtc gctctccgtg atcaggaaac
     2881 gaggcgacat tcgagacggc gtcgagcacc gccggatggt cgggcaggta gttcacgccc
     2941 agtttgatcc aggttcccag gtcgtcaggt g
//
LOCUS       DAORHD010000558         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38510_length_2970_cov_3.091938, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000558 DAORHD010000000
VERSION     DAORHD010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38510_length_2970_cov_3.091938"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2970)
                     /locus_tag="PKO06_12075"
     CDS             complement(<1..>2970)
                     /locus_tag="PKO06_12075"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70428.1"
                     /translation="VVGSLDGEVRLWKLRDDEQVDWVLGVVDRPVAQVEEIRKTIPHA
                     YVLIVNTPTECVIGEQRQAVDELVRRLGCSYFGIEGVSSVHNEAATPVAQAYRALHLF
                     ETNPPDDIAFYSSGWARRFAPSREEAADSILAQAVGTIDYPKVIEQAYADGARLFVEV
                     GPKASCSRMIGRILGDRPFMARSASLPGQGEASSILRLVAHLQAEGVVVDPTILYQTP
                     EAEAQSFTTNTDSGNRRIVVTIGCEPDWTQLSRPGTRPLAATVPVSPSPVRSSEPLAT
                     RPAVNSTPKATSVPLPAQSPLPSAQPASRVVGENSRPTPEAATQENPPAAPLRPAEIA
                     STIRPTVQTPVNTLISSAQPILSESSGTPQVERPGQPRPCDSGSIAFAQKLTTRGEVS
                     AGAAPACPITSPVLQQMVQVQSQTAAAHAAYLRTAEILRQAHLALLQGGALPPASGVE
                     PLAVQGSMMARVVQAVPSSPATVLESLGPQMATEAEKQSKQPVQTISPGLPSSAVAQT
                     EGPEKPVARVATSAKLQKPVRHDACEQTVFLDRLGSVEFGIGRIGAALGDFFAPIDQH
                     PTRVRLPGDPLNFCDRIVLVEGEKGSLKSGRVVTEHDVIPEGWYLDGGCMPTGLSVEA
                     GQADLFMSSWLGIDFKTKGLAMYRLLDAVVTIHGPLPRPGQTIRYDIKVDRFIRQADT
                     YLFFFQFDGTIDGKHLITMREGCAGFFTQKQLDEGKGIVLTDDDLRVETRPRPPVIPG
                     LPAPAITSLDDQALAALRVGDLETAFGPEFAGLPLRKPLTIPSGKLAMIDRVTSIEPN
                     GGRFGLGLIKAEIDIDPDAWFLTCHFTDDMVMPGTLMYESCLQALRVFLLRLGWVAET
                     GECFYEPVPEVKSRLRCRGQVIRGVKRALYELTIKQIGYGPEPYAIADALMFADGRMI
                     VQVTDMSIKLTGSSPELLRTIWAARKPTRHPQTLLPELAHAAHLASVMVYDRRDPIIT
                     YEQVEQFSIGKPSLCFG"
BASE COUNT          599 a          925 c          892 g          554 t
ORIGIN      
        1 ccccgaaaca gagggagggc ttgccgatcg agaactgctc gacctgctcg taggtgatga
       61 tcggatcgcg cctgtcgtag accatgacag aggcaagatg tgcggcatgc gccagttcag
      121 gcagcaacgt ttgcgggtgc cgcgtcggtt tgcgagccgc ccagatggtg cgcagcagtt
      181 cgggcgatga accggtcagc ttgatcgaca tatcagtgac ctgcacgatc atgcgaccat
      241 cggcaaacat caaggcatca gcgatggcat aaggctctgg cccgtagccg atctgtttga
      301 tcgtcaactc gtacaacgca cgcttgacgc cgcgaatcac ctgtccacga cagcgcaatc
      361 ggctcttgac ctctgggacc ggttcataga aacactcgcc ggtctcagcc acccacccca
      421 gtcgcaacag gaacacacgt aacgcctgga gacacgactc gtacatcagg gtgccgggca
      481 tgaccatatc atcggtgaag tgacaggtca ggaaccacgc gtctggatcg atgtcgatct
      541 cggctttgat gagtccgagt ccgaaccggc caccattagg ttcaatcgag gtgacgcggt
      601 cgatcatggc cagcttgcca ctgggaatcg tcaatggctt gcgcaacggc aaccccgcga
      661 attcggggcc gaacgccgtt tcgagatcgc ctacacggag agccgcgagg gcttgatcgt
      721 ccaggctggt gatcgcggga gccggcaatc cgggaatgac tggcggtcgc ggtctggtct
      781 ccacacgcag gtcatcatca gtcagaacga ttcccttgcc ttcatcaagt tgtttctggg
      841 taaagaaccc ggcacaccct tcgcgcatcg tgatcaaatg tttgccatcg atcgtcccgt
      901 caaactgaaa gaagaagagg taggtgtcgg cctgacgaat gaaacgatca accttgatgt
      961 cataccgaat ggtctgaccg gggcgcggca atgggccgtg aatcgtcacg acggcatcga
     1021 gcaaccggta catggccagg cccttcgtct tgaaatcgat gccaagccac gacgacatga
     1081 acaggtcagc ctgcccggcc tcgaccgaca aaccggtcgg catgcagccg ccgtcaagat
     1141 accagccttc cggaatgacg tcgtgttcgg tgacgacgcg cccgctcttg agactgccct
     1201 tctcgccttc gacaagtacg atacgatcgc agaagttgag cggatcaccc gggagtctca
     1261 cccgagtcgg atgctgatcg ataggggcga agaagtcccc cagcgcggca ccgattcgcc
     1321 cgatgccaaa ctcgacactg ccaagtcggt cgagaaatac ggtttgctcg caggcatcat
     1381 gtcgcacggg tttctgcagc ttcgcggatg tcgccaccct ggcgacgggc ttctccggcc
     1441 cttcggtctg cgcgactgcc gacgacggca acccggggga aatcgtctgt accggttgtt
     1501 tcgattgctt ttctgcttca gtcgccattt gcggccccag agattcaaga accgtcgctg
     1561 gcgaagaagg aacagcctgc actacccgag ccatcatcga cccctgtacc gcgagcggtt
     1621 cgacccctga cgccggcggc agcgcaccgc cctgcaacag ggccagatgt gcctgtcgca
     1681 gtatctcagc tgttcgcaga tacgccgcat gagcggccgc cgtctggctc tgcacctgca
     1741 ccatctgttg cagcaccggt gacgtaatcg ggcacgccgg agccgcacca gcgctgactt
     1801 ctccgcgagt cgtcagcttc tgagcgaagg cgatcgagcc gctatcgcac ggcctcggtt
     1861 gtccagggcg ctcgacctgt ggagttcccg aactctccga cagtatcggc tgagctgatg
     1921 agatgagggt gttcacgggt gtctgcacgg tgggccggat cgtactcgcg atttccgcag
     1981 gccggagagg cgccgctggc gggttttcct gagttgcagc ctcgggggtc ggccgtgagt
     2041 tttcgcccac cacccgtgaa gcaggctgtg cagaaggcag aggtgactga gcaggcaggg
     2101 gaacagaagt cgccttcggc gtgctgttca cggccggccg cgtggcgagc ggctcactcg
     2161 agcgaacagg agaggggctc acgggaaccg tcgccgccag gggacgtgtg cccggtcgac
     2221 tcagttgtgt ccagtcaggt tcgcacccga tcgtgaccac gatacgtcga tttcctgaat
     2281 ctgtattcgt ggtgaatgac tgcgcttctg cctcgggtgt ttggtacaaa atcgtcggat
     2341 cgacgacgac tccctcggcc tgcaggtggg ccaccagccg cagaatcgag ctggcctcgc
     2401 cctggccggg gagcgacgcg gatcgcgcca tgaacggccg atccccgaga attctcccga
     2461 tcatgcggga acaggaagct ttcggaccca cctcgacgaa cagacgcgcc ccgtcagcgt
     2521 aggcttgctc aatcactttc gggtaatcga tcgtaccgac ggcctgcgcc agaatcgaat
     2581 ctgccgcctc ttcgcgtgag ggggcgaaac gccgcgccca gccactgctg tagaaggcga
     2641 tgtcgtcagg cggattcgtt tcgaacagat ggagcgctcg ataggcctga gccaccggtg
     2701 tcgccgcctc gttatgcacc gacgagaccc cctcgatgcc gaagtacgag caaccgagtc
     2761 ttctcaccag ctcgtcaaca gcctgacgct gctcgccaat cacacactcc gtaggagtat
     2821 tgacgatcag cacataggca tgcggaatcg tttttcgaat ctcctcgacc tgagcgacgg
     2881 ggcgatcgac gacaccgagc acccagtcga cctgttcgtc gtcgcgcagc ttccagaggc
     2941 ggacctctcc gtccaacgaa cccacgacga
//
LOCUS       DAORHD010000559         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38618_length_2965_cov_3.357732, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000559 DAORHD010000000
VERSION     DAORHD010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38618_length_2965_cov_3.357732"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PKO06_12080"
     CDS             <1..209
                     /locus_tag="PKO06_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70429.1"
                     /translation="GGIIYLQLTDAKFSVGILLAAVGLFILFHGVSVKNVLGSGTKQE
                     KPVVKVPPKPKPTWKIEVASPTSR"
     gene            410..1045
                     /locus_tag="PKO06_12085"
     CDS             410..1045
                     /locus_tag="PKO06_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70430.1"
                     /translation="MADTKPRFEFRAFAQNFGMVTEKMRRLSPIDKIRESSEIYIMSA
                     GNNENNTKIRDDLMDIKVFVKEEKGLQQWNPRMKGSFPMKAETIEKEVFPAFGVAVPK
                     FGRPEYTLSQFVNEVIRPHKDLVAAHVFKRRYGFMVNECITEYAELLINGAAIQTVAV
                     EAVDLEAILKAKEMLGLQDYENVNYLMAIKRITGMEPAPAHWSLGLAQYLK"
     gene            1058..1525
                     /locus_tag="PKO06_12090"
     CDS             1058..1525
                     /locus_tag="PKO06_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HNV70431.1"
                     /translation="MGKEIERKFLVQGDDWKKLAKGTTYRQGYLNSTKERTVRVRTID
                     DKGFLTIKGVTVGATRAEYEYEVPGRDADEMLTNLCEKPLIEKRRYKIPQGNLTWEVD
                     EFLGDNNGLIVAEIELPEEGTKFDKPAWIGKEVTGDPKYFNSNLTKNPFKNWK"
     gene            1535..2464
                     /locus_tag="PKO06_12095"
     CDS             1535..2464
                     /locus_tag="PKO06_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70432.1"
                     /translation="MTLRLVIFLLMTGLLGQLAHAAGPAHQRDPQLSARECKMLLRPE
                     RFADRSAGVRELLKLVREACSISGLVFITANDTEAESVRFVSFLDTPKFDLYKRGYVL
                     RHRQDALVDRYGKCRVATKKYELTFKFRHPEPTVAIEQDTTPHSGFQGKESFEENLLV
                     KETVIDRVYDRSGKVELKNKLGKKISDFSPIFPGLGKVGLSGSEEIVRVNELEIEEYQ
                     RDLGTIELGTRATGKAVVSVWYRRGEAKPLIAEFSFKTRLLSRDRLHSLINHTYRTSE
                     TLLRELQKAGSTWIAVGTTKTGLVYQMSQPNQD"
     gene            complement(2490..>2965)
                     /locus_tag="PKO06_12100"
     CDS             complement(2490..>2965)
                     /locus_tag="PKO06_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70433.1"
                     /translation="KPLRPRVFRQTLLSLRQGLPTEQRDRHPGATVSTARRILVVEDE
                     PVNQMLMTMLLKRAGHEVETQEHGSAALERLRTGVFHLVFMDISMPEMNGIEVTWRAR
                     KEGCTTPIIICSANAMPEDLAAARSAGANGFLKKPYSFEELQHMIVRHAPQALTP"
BASE COUNT          703 a          777 c          842 g          643 t
ORIGIN      
        1 ctgggggaat catctacctg caactcaccg atgcgaagtt ctcggtcggc attctccttg
       61 ccgccgtcgg tttgttcatt ttgttccacg gggtgtcggt gaaaaacgtg ctgggttcag
      121 gaacaaaaca ggaaaaaccc gttgttaaag tgccaccaaa gccgaagccg acctggaaga
      181 tcgaggtggc ctcgccaacg tctcggtgag acttgttgtc ttgctcactg ccccagtcac
      241 cgccgctcga ccgaacattc tgacaaacct gtacgtccgt gtcgagtgga tgatcgatgc
      301 cgtcgaccag gcaagtctga attgaagccg tggttgcttg aactctcacg cgtgaagtat
      361 gctcattccg gatcaacgac tatttccctt tactgaagga gtgcttccaa tggccgacac
      421 gaaacctcgt tttgaatttc gagcctttgc ccagaacttc ggcatggtca ccgaaaagat
      481 gcgccggttg tcgccgatcg acaagattcg agaaagcagc gagatctaca tcatgtccgc
      541 tggcaataac gaaaacaaca cgaagattcg tgacgatctg atggatatca aagtgttcgt
      601 caaagaagaa aaagggcttc aacagtggaa tccacgcatg aagggcagtt ttcccatgaa
      661 ggcggaaacg atcgaaaagg aagtgtttcc cgcctttggt gttgccgttc cgaagtttgg
      721 acggcccgag tacaccttgt cccagtttgt caatgaagtc attcgccctc acaaagatct
      781 ggtggcggct cacgttttca agcgccggta cggtttcatg gtcaatgagt gcatcaccga
      841 atatgccgaa ttactgatca acggggcggc aattcaaacc gtggccgtcg aagcggtcga
      901 cctcgaggcg attctgaaag ccaaagagat gctgggcttg caagactacg agaacgtgaa
      961 ctacctgatg gcgatcaagc gcattaccgg catggagccc gcacccgccc actggagcct
     1021 cggactggct cagtatctga agtaaaggaa atcagccatg ggaaaagaaa tcgaacgcaa
     1081 gtttctggta caaggggatg attggaagaa gctcgccaaa gggacgacct atcggcaggg
     1141 ctatctgaat tcgacaaagg aacgaacggt tcgagttcgt acgattgacg acaagggctt
     1201 tctcacgatc aagggggtga ccgtcggtgc cacgcgtgcc gagtatgagt acgaagttcc
     1261 cggtcgtgat gccgacgaaa tgctgaccaa tctctgcgag aagccgttga tcgaaaaacg
     1321 gcggtacaag attccccagg gcaatctgac ctgggaagtc gatgaattcc tgggtgacaa
     1381 taacggtctg attgtggctg agatcgaact ccccgaagag ggaacgaagt tcgacaagcc
     1441 tgcttggatc ggcaaagaag tgacgggcga tccgaaatac ttcaattcca atctgacgaa
     1501 gaaccctttt aagaactgga agtaggagcg acgtatgacc cttcgcctgg tgatcttcct
     1561 gctgatgaca gggcttctgg gacaactggc tcacgcggca ggtccggctc accagagaga
     1621 tccgcaactg agtgcgcgcg agtgcaagat gctgctccga cccgagcgat ttgccgatcg
     1681 ctccgccggg gtcagggagc ttctcaaact ggtacgtgag gcgtgcagca tttcggggct
     1741 ggtattcatc accgcgaacg ataccgaagc cgagtctgtt cgtttcgttt ccttccttga
     1801 taccccgaaa ttcgacttgt acaagcgcgg gtatgtgctg agacatcgtc aggatgcgct
     1861 cgtcgaccgg tatggaaagt gtcgggtcgc cacgaagaag tacgagttga ccttcaagtt
     1921 ccgccatccg gaaccgaccg tcgccatcga gcaggacacc acccctcaca gcggatttca
     1981 gggcaaagag tcgtttgagg aaaatctgct cgtgaaagag accgtcattg accgcgtcta
     2041 tgatcgcagt ggcaaggttg aactgaagaa caaactggga aagaagatct cggacttttc
     2101 accgatcttt cccggtttgg gaaaagtggg cctcagcggt tcagaggaaa ttgtccgggt
     2161 gaacgagctc gaaattgagg aataccagcg tgatctgggc acgattgaac tcggaacccg
     2221 ggcaaccggg aaagcggtgg tctcggtctg gtatcgtcgt ggagaggcga aaccgctcat
     2281 tgccgaattc tcattcaaga ccaggctgct gtcgcgcgat cgtctccact cgctgatcaa
     2341 ccacacctac cgcacgtcgg aaacgctgct gcgcgaactg cagaaggctg gtagtacctg
     2401 gatcgcggtc ggaaccacga aaaccggtct tgtctatcag atgtcgcagc cgaatcagga
     2461 ctgacttcca tctccggctt tgtctgggct cacggcgtca atgcttgtgg agcatgtcgg
     2521 acgatcatgt gttggagttc ctcgaaactg tagggttttt tcagaaaccc gttcgctcct
     2581 gcggatctgg cggcggccag gtcttcgggc atggcgtttg cgctgcagat gatgatcgga
     2641 gtcgtgcatc cttctttgcg tgcccgccag gtcacctcga tgccattcat ctccggcatc
     2701 gatatgtcca tgaacaccag atgaaacacg ccggtgcgca ggcgctcgag ggcagccgac
     2761 ccgtgttcct gggtttccac ttcatgaccg gcccgcttca gtaacatggt catgagcatc
     2821 tgattgaccg gttcatcttc gaccacgaga atacgacgag ccgtcgaaac agtcgctccc
     2881 gggtgacggt cccgttgctc ggtgggcaga ccttgtcgca gactcagcag tgtctgtcga
     2941 aacactctcg gccgaagggg tttgc
//
LOCUS       DAORHD010000560         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38739_length_2959_cov_3.877066, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000560 DAORHD010000000
VERSION     DAORHD010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38739_length_2959_cov_3.877066"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKO06_12105"
     CDS             complement(<1..318)
                     /locus_tag="PKO06_12105"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HNV70434.1"
                     /translation="MEQLKAVIDHLRKQGVRFADAREHLFEERGLRFEDRRVSNLTEE
                     YTNGVGIRVLYENGWGYASTPSLTAEGIRKAADEALALAKSASAQATRTIEFCEEPRH
                     IATF"
     gene            502..1410
                     /gene="folD"
                     /locus_tag="PKO06_12110"
     CDS             502..1410
                     /gene="folD"
                     /locus_tag="PKO06_12110"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNV70435.1"
                     /translation="MSTILDGKKIAGEIAAEVGNKVREWKTASGKQPGLAVVLVGEDP
                     ASKVYVSRKKKACEEAGLYSREVKLAAETTESALIAEIDRLNADAQIHGILVQLPLPS
                     HIDEQKILERVHPDKDVDGFHPINMGKLLAGADTFLPCTPYGVMELLRRSNIDPLGKR
                     ALVVGRSTIVGKPVAQLLMAKHATVTIAHSRTRDLEEEVARAEILVAAVGKAQMIKGS
                     WVQPGAVVIDVGTNMIVDPTHPKGQRLVGDVEYETAATRAAFITPVPGGVGPMTIAML
                     LVNTLKAFSQQVLHTPFSFEEMSRGS"
     gene            1400..2563
                     /locus_tag="PKO06_12115"
     CDS             1400..2563
                     /locus_tag="PKO06_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNV70436.1"
                     /translation="MDLDILLMTPGPVTLDSEILLAGAQPLKHHRTNDFAPLYADCVA
                     RCKRIFKTKKHLFLTTSSGTGAMETAIANHFHPGEQVLTVETGSFGERFTEIAKSFRL
                     EAIPLKYPWGHRAKVKDIIRMLDQNPKIKGVTVTFNETSTGVRNDIEAIGKALKDRDV
                     LFITDGVSGIGALPFDMDGWHVDVAVSASQKGFLTPPAIGMIALSDRAYDKMMKVQCN
                     GYYFDLKHYKKNQDLAIPSYPWTPAISVMFSFQKALEKMERIGMEKIFAHYHKLAEGL
                     RKAIEAMGLSIFTQPDARSDVLTVINSPDGIHPSKIVKDLHDRYSVLIAGGQGEITDK
                     VFRITTIGTIGEREIVGTVGLLEIALARLKFLKEPGVGTAALLKAFAAMEAKE"
     gene            2573..>2959
                     /locus_tag="PKO06_12120"
     CDS             2573..>2959
                     /locus_tag="PKO06_12120"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNV70437.1"
                     /translation="MASKVRQDLQISQAATLKPITEIAAEIGLLPIEIEQYGPYKGKV
                     PLGTYQRLQNKPQGKLVLVSAMTPTPAGEGKTTTTIGLTDALRHRGKKAMLCLREPSM
                     GPVFGVKGGACGGGQAQVVPMEDINLH"
BASE COUNT          647 a          829 c          879 g          604 t
ORIGIN      
        1 gaaggtcgcg atgtggcgag gttcttcgca gaactcgatt gtgcgggtag cctgcgcgct
       61 ggctgacttc gccaatgcca gggcttcgtc ggcggctttc cgaatgcctt cggccgtgag
      121 cgacggtgtc gaggcgtagc cccagccgtt ttcgtagaga acccgtatcc cgaccccgtt
      181 cgtgtattcc tcggtcaggt tcgacacgcg ccggtcttcg aagcgcaatc cgcgctcctc
      241 gaacaggtgt tcacgcgcat ccgcgaagcg tacgccctgc tttcggagat gatcgatcac
      301 cgctttcaat tgttccatgt tgttccttga tagtgtgtga agtcgtcgct cacgttcgcc
      361 gctcagtcta cctgtccgag actgaaatgc caacctcatc taaggactgc cggatttctc
      421 gatttgacgt gatgtcgctt gtctcttgtc agtcagggaa gagcggagta tactcgcgcg
      481 aaaggaaagg gaatcacgtt catgagcacc atactggacg ggaagaagat tgcaggcgag
      541 attgccgctg aagtcggcaa taaggtcaga gagtggaaga cggcgagtgg aaaacaaccg
      601 ggactcgcgg tcgttctggt cggggaagac ccggcatcga aggtgtatgt ctcgcgcaag
      661 aagaaagcct gcgaagaggc tgggctgtat tcgcgtgagg tcaagctcgc ggccgaaacg
      721 acggaatcgg cactcattgc cgagattgac cggctgaacg cagatgcaca gatacatggc
      781 attctcgttc aacttcccct accctctcat atcgatgaac agaagattct cgaacgcgta
      841 cacccggaca aagacgtcga cgggtttcac ccgatcaaca tgggaaaact gctggccggc
      901 gccgatacct ttctgccgtg caccccatac ggtgtcatgg aactgttgcg gcgttcgaat
      961 atcgacccgc tcggcaaacg cgccctggtc gtcggtcgca gcacgatcgt cggcaagccg
     1021 gtcgcccagc tgctcatggc caagcatgca acggtcacga tcgcccattc ccggactcgc
     1081 gatctggaag aggaggtcgc acgagcggaa attctggtgg ccgcggtcgg aaaagcgcag
     1141 atgatcaagg gaagctgggt gcaaccgggt gccgtggtca tcgatgtcgg tacaaacatg
     1201 atcgttgacc cgacccaccc gaaggggcaa cgtctggtcg gcgatgttga gtatgaaacg
     1261 gcggcgacgc gcgcggcctt catcactccc gttcccggcg gagtcgggcc gatgaccatc
     1321 gccatgctgc tggtgaatac tctgaaagcc ttttcacaac aggttttaca caccccattt
     1381 tcattcgagg agatgagccg tggatcttga tattcttctg atgacgccag ggccggtaac
     1441 ccttgattcc gagattcttc tggcgggcgc tcagccgctg aagcatcatc gcacgaacga
     1501 ttttgctccg ttgtacgccg attgtgttgc tcgctgcaag cgcatcttta aaaccaagaa
     1561 gcatcttttt ctgaccacgt cttcgggtac tggggccatg gaaaccgcta ttgcgaacca
     1621 ctttcacccg ggcgagcagg tactgacggt tgaaaccggt tctttcggcg aacgcttcac
     1681 ggagatcgcg aagagttttc gtcttgaggc cattccgctg aagtacccgt ggggtcatcg
     1741 cgcgaaggtg aaagacatca tccgcatgct tgaccagaac ccgaaaatca agggtgtgac
     1801 ggtcacgttc aacgagacgt cgaccggcgt gcgcaacgac atcgaagcca tcggcaaagc
     1861 cctgaaagac cgcgacgttc tgttcatcac cgacggtgtt tccggaatcg gagcgttgcc
     1921 tttcgacatg gacggctggc acgtcgatgt cgctgtttcg gcttcgcaga aaggtttttt
     1981 gacgcctccc gccatcggca tgatcgccct ttccgatcgg gcgtatgaca agatgatgaa
     2041 ggtgcagtgc aacggctact acttcgatct gaagcactac aagaagaacc aggatctcgc
     2101 cattcccagt tacccctgga cgccggcgat ttcggtcatg ttttcatttc agaaagcgct
     2161 cgagaagatg gaacggatcg gcatggaaaa gatcttcgcg cactaccaca agctggcaga
     2221 aggcctgcgc aaggcaatcg aggccatggg tttgtctatc ttcacccagc cggatgcgcg
     2281 ctcagacgta ttgaccgtga tcaacagccc ggacggtatt catccctcga agatcgtcaa
     2341 agacctgcat gaccgttatt ctgtgctgat cgcgggcgga cagggcgaga tcaccgacaa
     2401 ggtgtttcgc attaccacga ttggcacgat cggggaacga gagatcgtcg gcacggtcgg
     2461 cctgctcgag atcgctctgg cgcgtttaaa gttcctgaaa gaacccggag tcgggactgc
     2521 ggcgctgctg aaggcgtttg ccgcaatgga agccaaggag taaggagaca tcatggcaag
     2581 caaggtgagg caagacctgc agatttccca ggccgcgacc ctcaaaccga ttaccgagat
     2641 tgccgccgag attggcctgt tgccgatcga gatcgagcag tacgggccgt ataaaggcaa
     2701 agtgccgctg ggcacgtatc agcgcctgca gaacaagccc cagggcaagc tggtactggt
     2761 atcggccatg acccccaccc ccgcgggcga aggaaaaacc acgacgacca tcggtctgac
     2821 cgatgccctg cgtcatcgtg gcaagaaagc catgctgtgc ctgcgcgagc cttcgatggg
     2881 gccggtgttc ggcgtgaagg gcggcgcgtg cggcggcgga caggcccagg tcgtgccgat
     2941 ggaggacatc aacctgcat
//
LOCUS       DAORHD010000561         2959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38742_length_2959_cov_3.457645, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000561 DAORHD010000000
VERSION     DAORHD010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38742_length_2959_cov_3.457645"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..628)
                     /locus_tag="PKO06_12125"
     CDS             complement(176..628)
                     /locus_tag="PKO06_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70438.1"
                     /translation="MKQTPDLQHIQEKMKAGTLTLKGFLGDDTRTLSDIIAADELIVA
                     SRGLSHEQIAAKLADLTERGRDLMEGEVELDGRFRVSVRDDRGKLPSPWGDGRFEKGD
                     TVLVELTTGRTWRWNQLTLHLVKNHGFYGGQGSDYRLDPQEMIALLGL"
     gene            complement(647..1747)
                     /locus_tag="PKO06_12130"
     CDS             complement(647..1747)
                     /locus_tag="PKO06_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNV70439.1"
                     /translation="MTEVFTSVFILTSISLVLVLLLLIAEYFFANYGECTIDINKGER
                     VLKVQGGNSLLRTLSETKLFLPSGCGGRGSCGTCKCKVVSGVGQVLPTEIPYLNPTEL
                     KENVRLACQVKVKQNVQIEVPEELLSVKEFETVIEKYVPLTHDIRGLRFKLPEGETVK
                     FKAGQFVNLVSEPYGDVTESTSRAYSISSAPSDNKALELIIRLVPNGIVTTYVFEHLK
                     EGAKAKLIGPYGEFFLRDSDREIICIAGGSGLAPIRSIVLDMIDRGISNRKCTFFFGA
                     VNVRDVYYVDEFKALEAKHPWFKFVPALSGKEEGHGFERGLITDVVARHYGTLDNHEA
                     YLCGSPGMIGACVKVLTGKGLPENRVFYDKFS"
     gene            complement(1759..2358)
                     /locus_tag="PKO06_12135"
     CDS             complement(1759..2358)
                     /locus_tag="PKO06_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNV70440.1"
                     /translation="MDIQAIHPLVILFAAITTNNILLTNFLGMCSFLAVSKELESSVG
                     LGVAVIFVSTLTAPLCWAVNTWVLVPYQLEYLQYIVFIMVVAAFVQIVEMIIERVSET
                     LYLTLGIFLPLITVNCCILGCILFLVIRQYTFWQSIAYGFGAGTGWAIAIVALAGIKE
                     RMKKRSKVIPELEGPGISLIITGIIAMAFVGFSGITKIN"
     gene            complement(2374..>2959)
                     /locus_tag="PKO06_12140"
     CDS             complement(2374..>2959)
                     /locus_tag="PKO06_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNV70441.1"
                     /translation="LWEDNPVVVQILGICSSLAVTNLVKNTLVMCLGLIFATAMSAWT
                     VSLLRNLMPRKVRMITQVLIIAAWVIVIKIILDAFLPAISKDLGPYVGLIITNCIVMG
                     RTEAFAMANRPWASFLDGIGAGVGYSLVLLTIAVFREPLGLGTLFGFQVIPDGWTRWN
                     MMVIAPSGFFCLATFIWVARAMKAPAPGAAPVKK"
BASE COUNT          640 a          843 c          891 g          585 t
ORIGIN      
        1 tccgacggcg cagaaagaaa ccagcgatgc cggtgtcgat atgaaagcct atcaggtgca
       61 gttgtcgggc ggcaaaggtc cgtatctcaa cctggacgat ttcaagaaac ctctgccgaa
      121 agaggtcgac gtgttcaaga ccatcgacag cgtctgggac ggcggttgag ctgagctaca
      181 gacccagcag ggcgatcatc tcctgtgggt cgagacgata atcgctgccc tgaccgccat
      241 agaaaccatg attcttcacc aggtgcaggg tcagctggtt ccagcgccag gtacgacccg
      301 tcgtcagttc cacgagcacc gtgtcccctt tttcgaaacg cccgtcaccc caggggctgg
      361 gcagtttgcc gcgatcgtcg cgcacgctga cgcgaaagcg tccgtccagt tcgacttcgc
      421 cttccatcag gtcgcggccg cgttcggtga ggtcggcaag tttcgcggcg atctgctcgt
      481 gcgacaagcc gcgactggcc acgatcaatt catcggcagc gataatgtct gaaagagtcc
      541 gggtatcgtc accaagaaac cctttcagag tcagggtgcc ggctttcatc ttttcctgaa
      601 tgtgctgcag gtcgggtgtc tgtttcatgg ggctctctcc tctcgctcag gaaaacttgt
      661 cgtagaagac gcggttctcg ggcagaccct tgccggtcag caccttgacg caggcgccga
      721 tcatgcccgg actgccgcac agataggctt cgtggttgtc gagtgtgccg tagtgtcggg
      781 ccacgacatc ggtgatcaga ccgcgttcga acccgtgccc ttcctctttg cccgacagag
      841 ccggcacgaa cttgaaccac gggtgcttcg cctcgagagc cttgaactca tcgacgtaat
      901 acacgtcgcg cacgttgacg gcaccaaaga agaacgtgca ctttcgattg ctgatgccgc
      961 ggtcgatcat gtcgagcacg attgaacgaa tcggcgccag ccccgagccg ccggcgatgc
     1021 agataatctc gcggtcggaa tcgcgcagaa agaactcgcc gtacgggccg atcagcttcg
     1081 cttttgcacc ttccttgaga tgttcgaata cataggtcgt gacgatgccg ttcggcacca
     1141 ggcgaatgat cagttcgagc gctttgttgt cggatggggc cgacgagatt gaataggcgc
     1201 gtgacgtcga ttcggtgacg tcgccgtagg gctctgagac taggttgacg aactgcccgg
     1261 ccttgaattt gaccgtctcg ccttcgggca gcttgaaccg caggccgcga atgtcgtgcg
     1321 tcagcggcac gtacttctcg atcacggttt cgaattcctt gaccgaaagc agttcttcgg
     1381 gaacttcaat ctggacattc tgcttgacct tgacctggca ggccaggcgc acgttctcct
     1441 tcagctcggt ggggttgaga taggggattt cggtcggcag cacctgaccg acgcccgaga
     1501 cgaccttgca cttgcaggtg ccgcaactgc ctctgccgcc gcagccagaa ggcagaaaca
     1561 gcttggtctc ggaaagcgtg cgcagcagac tgttgccgcc ctgcaccttg agcacgcgtt
     1621 cgcccttgtt gatgtcgatc gtgcattccc cgtagttggc gaagaaatac tcggcgatca
     1681 gcagcagcaa aaccaggacc agtgagatgg aggtgagaat gaagacgctg gtgaatactt
     1741 ccgtcatggt cgatagtcct agttgatttt ggtgataccc gagaacccga cgaaggccat
     1801 ggcgatgatc cccgtaatga tcagagagat acccgggcct tcgagttcag gaatcacctt
     1861 cgaacgcttt ttcatgcgct ccttgatgcc tgccagagcg acaatcgcga tggcccagcc
     1921 ggtacccgcg ccgaacccgt aggcgatcga ctgccagaag gtatactgcc gaatcaccag
     1981 aaacagaatg cagccgagaa tgcagcagtt caccgtaatc aggggcagaa agatgccgag
     2041 cgtgagatac agcgtttcgc tgacccgttc gatgatcatc tcgacaatct gcacaaaggc
     2101 cgcgacaacc atgatgaaca cgatgtattg cagatattcg agctggtacg gcaccagcac
     2161 ccaggtattg accgcccagc acagaggcgc ggtcagggtc gagacgaaga tgacggcgac
     2221 gcccagcccg accgaacttt cgagctcttt cgacacggcg agaaacgagc acatgccgag
     2281 aaagttggtg agcagaatgt tgttggtcgt gatcgccgcg aacagaatga ccagtgggtg
     2341 aatggcttga atgtccatgg tggtctcctt cgcctacttc ttcaccggcg ctgcgccggg
     2401 ggccggagcc ttcatggctc gtgcgaccca gatgaaggtc gcgagacaga agaagccgct
     2461 gggggcaatg accatcatgt tccagcgggt ccagccgtcg ggaatgacct gaaacccgaa
     2521 cagcgtgccc aggccaaggg gttcgcggaa aaccgcgatg gtcagcaaca ccaggctgta
     2581 tccgacgccg gccccaatgc cgtcgaggaa cgaggcccac gggcggtttg ccatggcgaa
     2641 ggcttcggtg cgccccatga cgatacagtt cgtgatgatc aggccgacat acgggccgag
     2701 atccttgctg atcgcgggca ggaatgcatc gagaatgatc ttgatgacga tgacccaggc
     2761 ggcgatgatc agcacctgcg tgatcatgcg cactttccgg ggcatgaggt ttcgcagcag
     2821 ggagacggtc caggctgaca tggcggtggc gaagatcagg cccaggcaca tgaccagcgt
     2881 gtttttgacc agattggtga cggcgagcga cgaacagatg ccgagaatct gcacgacgac
     2941 cgggttgtct tcccagagg
//
LOCUS       DAORHD010000562         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_38917_length_2950_cov_3.357513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000562 DAORHD010000000
VERSION     DAORHD010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38917_length_2950_cov_3.357513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKO06_12145"
     CDS             <1..887
                     /locus_tag="PKO06_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70442.1"
                     /translation="LLKSEIRRECPDVIVYVCKAKEVDANVRFDLEFLNQVVEFTRKT
                     YAVDSCPVLAVVNQVDELDPAHVTTPPFEDPVKRENMQRAVEHLGEMFKMHYRYYADD
                     KIVIRPICSYMYFQDGHLACDRRWQIDELLDEILALLPDEALLSMMKLGRFQEVRRKL
                     SNRIVNTFTMATGIIGAEPIPYADLPILTSLQVSMIGMIQAVAGRGASMRNIRDFLAA
                     TGINITGAFTVREVVRALVKLLPGAGSLISGTVAAGYTKLIGTTAINHFIEGDEIGKA
                     TAKAEQATGRAWTRGVSA"
     gene            980..1156
                     /locus_tag="PKO06_12150"
     CDS             980..1156
                     /locus_tag="PKO06_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70443.1"
                     /translation="MMPNHKAVLDQTIRLHGCLPAWYRLPHFLARVIYELRQGKSLPQ
                     AIFEALIENGGPYA"
     gene            1153..>2950
                     /locus_tag="PKO06_12155"
     CDS             1153..>2950
                     /locus_tag="PKO06_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70444.1"
                     /translation="MNDVTQEAAGVPAETACRPVHDPQRRGPAELRRARLMRLFCRRW
                     CESGFAPRAAVTLLRLLAFLHRVRGLTIEEMTSWLEYRGLAIDEGQVAETIGRLGRHL
                     VVDEAGRYSLKNTSLARCMRRRLGRSIVRDAFERQVVGWLGETIAVDLDVTEQALRLV
                     YWRSITERTTHDLWPARLAKGFVGWSGERLRELADPLRGCEVFALALFDLAARKGGQA
                     ERYAYAIFLSLYDRFHAGRAFAPWFEAMRAWRLIRLSSTELLWELAREGHAPACCRLL
                     QDNDSTGSPRARRRRRQMNETMSERHRSGLRREFRDLLDVAVTDSSADMLYKLAMVSG
                     VFSSDEEWARILRVAADKGSREACHYLGELCHERPEIVPMQHRSWLKWAMQGLGLKSL
                     TGQAVRRLQLDFMAFLRLLLLAVRLMEGEAGDVLKQRGKEMTLAWAESSTADQGVWTA
                     ESHVEFGYHDLAELVTERAFARVQYPKIWERTADDLIETGTIYADLPGEDFWNPIRLF
                     NGFTALLFASGLALRNGSPRGLDLAGRIVASLGIRPEPVRILHQMIRRRLSSRWPPNA
                     GDDLSREAELLHAVWFWGASQIRRDGADAALPE"
BASE COUNT          577 a          808 c          974 g          591 t
ORIGIN      
        1 aactgctcaa gtctgagatc agacgcgaat gcccggatgt gattgtctat gtctgcaaag
       61 cgaaagaagt tgacgcgaat gtacgattcg atctcgagtt tctgaaccag gtggtcgagt
      121 tcacccggaa gacgtatgcc gttgactcgt gcccggttct cgcggtcgtg aaccaggtgg
      181 acgaactcga cccggctcat gtgaccacgc cgccattcga agacccggtc aagcgagaga
      241 acatgcaacg cgcggtcgag catctgggcg agatgttcaa gatgcactac cggtactatg
      301 ctgacgacaa gatcgtcatt cgaccgatct gttcatacat gtactttcag gatggacatc
      361 tggcgtgcga tcgacgctgg cagatcgacg aactgctgga tgagatcctc gcgctattgc
      421 ctgatgaagc cctgctgagc atgatgaaac tcggacgctt tcaggaagtg cggcgcaagc
      481 tgtcgaatcg catcgtcaac accttcacca tggcgacggg aatcattggt gccgagccaa
      541 ttccgtatgc ggatctgccc attctgacgt cgttgcaggt gtcgatgatc gggatgattc
      601 aggcggtggc ggggcgtggc gctagcatga gaaatattcg cgactttctg gccgcgaccg
      661 gaatcaatat taccggggct ttcacggtgc gtgaagtggt gcgagctctc gtcaagctct
      721 tgcccggcgc aggcagtctc atcagcggta cggtggcggc cggatacacg aaactaatcg
      781 ggacgacggc gatcaatcac ttcatcgagg gtgatgagat cggcaaggct accgccaagg
      841 ccgaacaagc gaccggcaga gcctggactc gcggggtgtc cgcataggtg agtcgaccca
      901 gccgctgacg gaagtgagtt gccctcggcg gcatgttccg aaggcagatg acgaacgagg
      961 attccaagga gaaagaccta tgatgcctaa ccacaaggct gttcttgatc agacgattcg
     1021 tttgcacgga tgtctgccgg cctggtatcg cctgcctcac tttctcgccc gcgtcatcta
     1081 tgagcttcgg caagggaagt ccctgccgca ggcgatcttc gaggccttga tcgagaacgg
     1141 cgggccctac gcgtgaacga cgtgacccag gaagccgcgg gcgttccggc ggaaaccgct
     1201 tgccggccgg ttcatgaccc tcagcgacgt ggcccggccg agctcagacg ggcccgattg
     1261 atgcgcctct tttgcaggcg atggtgcgaa tccgggttcg cgccgcgggc ggcggtgacg
     1321 cttctgcgtc tgctggcgtt tctgcatcgg gtgcgtggac tgacgatcga ggagatgacg
     1381 agctggctgg aatatcgcgg ccttgcgatc gacgagggtc aggtcgcgga aacgatcggc
     1441 aggctggggc ggcatctcgt ggtcgacgag gccggtcgct actctctgaa aaacacatca
     1501 ttggctcgtt gcatgcgaag acgactcgga cgttcgatcg tgcgcgacgc cttcgaacga
     1561 caggtcgtcg gctggctggg ggagacgatc gccgtcgatc tcgacgtgac tgaacaggct
     1621 ctgcggctgg tctactggcg gtcaatcacc gagcggacca cgcacgatct ctggccggcg
     1681 aggctggcga agggctttgt cggctggtcg ggcgaacgct tgcgagagtt ggccgacccc
     1741 ctgcggggtt gcgaagtgtt cgctctggct ctgttcgacc tcgcggccag aaagggcggg
     1801 caggcggaac gctacgccta tgccatcttt ttgtcgctgt acgatcggtt tcacgcggga
     1861 agagccttcg cgccgtggtt cgaggcgatg cgtgcctggc gtctgatacg actctcgtca
     1921 acggaactgc tctgggagct cgcccgagaa ggtcatgcgc cagcctgctg tcgattgttg
     1981 caggacaacg actcgaccgg ttcacctcga gcgcggcggc gacgccggca gatgaatgag
     2041 acgatgagcg aacgtcatcg aagcggtttg cgccgagagt tccgtgacct gctggatgtt
     2101 gccgtcaccg actcgtccgc agacatgttg tacaagctgg caatggtcag cggagttttc
     2161 agtagcgatg aagagtgggc gagaatactg cgggtggcgg cggataaagg ttcgagagag
     2221 gcctgccact atctcggcga actctgccac gagcgccccg aaatcgtgcc gatgcaacat
     2281 cggtcatggc tgaagtgggc gatgcaggga ctgggactca agagcctcac ggggcaagcg
     2341 gtgcgccgcc tgcaactcga cttcatggca ttccttcgtc tgctcctgct ggcggttcgg
     2401 ctgatggaag gagaggcggg tgatgtcttg aaacagcgcg ggaaagagat gactctggcc
     2461 tgggccgaaa gttcaacggc ggatcaaggc gtctggacgg cggagagcca cgtcgagttc
     2521 ggataccatg acctggccga gcttgtcacg gagcgcgcat tcgctcgggt ccaatacccg
     2581 aaaatatggg agcgaacagc ggatgacctg atcgaaaccg gtacaatata cgccgatctg
     2641 cccggtgaag atttctggaa cccgattcga ctgttcaacg gattcacggc tctgctgttt
     2701 gcgtccgggc tggctctcag gaacggctcg ccgcgcgggc tcgatctggc gggccgaatc
     2761 gtggcgtctc ttgggataag acccgaacca gtgaggattc tgcaccagat gatacggcgt
     2821 cgcctttcct cgcgctggcc accgaatgcc ggtgacgatc tgtctcgcga ggcggagttg
     2881 ttgcatgcgg tctggttctg gggcgcgtcg cagattcgtc gagatggggc ggatgcggct
     2941 cttccagaga
//
LOCUS       DAORHD010000563         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39106_length_2942_cov_3.157603, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000563 DAORHD010000000
VERSION     DAORHD010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39106_length_2942_cov_3.157603"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1424)
                     /locus_tag="PKO06_12160"
     CDS             complement(87..1424)
                     /locus_tag="PKO06_12160"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV70445.1"
                     /translation="MKKPFPFLYDVLSNGVQVAAESFDNVQSVSIGFFFKSGVLYEKL
                     ERLGASHFIEHMLFKGTKKRSPKDIARQFDRIGGHLNAFTSKDYCCYYARVVKDNLDE
                     AVEVLADMVCNSTFDKTELERERGVILEEIKMYEDSPDELIHELLGLSLWHNSPLGRP
                     ILGTSATVGGMTRTQIVEIYERQYVAENLMVCAAGNVDWTRLLKLLTKHLSGLRTGNF
                     RSRKEIPEPCYEVKIFQKEIEQVHVALGAPGTSYGDSRRYALTLLNTVFGGGMSSRLF
                     QEIREKRGMAYSVYSYHTAFRDSGIFAIYAGTSLEHLQRVLDLVHKEVEKVAKHGLNK
                     TELAEAKQQIRGNLLIGMESTTNRMNRLVTEFLYDFPPQNPTSAIEPYMAVTQADIIE
                     VAREILNEKRMAMTVISPDPRAAEIIRQSPFSRRPITSLPISERDRPTRSRTK"
     gene            complement(1433..2260)
                     /gene="scpB"
                     /locus_tag="PKO06_12165"
     CDS             complement(1433..2260)
                     /gene="scpB"
                     /locus_tag="PKO06_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNV70446.1"
                     /translation="MKIDEPLQVPQTVPDNVISLTEAQERRQQLYAALEGLLFVHGKP
                     LSVQLLGETLGVAPEEAMPLILAVKERYDSDPQRGLQIVINEQGVQLATKASIATFIQ
                     KLEGQRLVNLSLPALETLSVIAYKQPITRAEVEAIRGVNCDGVMSTLLEKKLIYVSGE
                     KPVIGKPRLYSTTSDFLYYFGIKSLKELPVPAVDVDPAALKEAAAAEAPQLEVEGVVA
                     ASAPPASTETTPEGSVQVPTGGDDPSANASSESGAPQTETETDQASHTSEAVLPRKE"
     gene            complement(2257..>2942)
                     /locus_tag="PKO06_12170"
     CDS             complement(2257..>2942)
                     /locus_tag="PKO06_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNV70447.1"
                     /translation="LEYLHLMEIFDMDVASEFLVMAATLLEIKSKMLLPKEKPVDLQP
                     GDEGYDEAQFDDDPRHDLVEKLLEYRRFKLLAMQMRELERQNSRIYTRGIDFEPQPQE
                     FLQISLGAVDLLNLFQSLVRRRLNPPVHRVVIDKVSLAERIKEIREYLTTLKRATTFR
                     ELLKDPQNRYEIVLSLMAVLEMAKGGELRVTQKGNFHPIHLRPAAAAAVDGDEEAADD
                     TSEEMTATA"
BASE COUNT          570 a          818 c          882 g          672 t
ORIGIN      
        1 ggtttcgtga cggtcctggc gcgagccgtg gtgccggcct tcttggggtt cgctttcgtg
       61 gtgcgaggca tgaaaagtct cctttgttac ttcgtgcgtg atcgggtcgg gcggtcgcgt
      121 tcgctgatcg gcagcgaggt gatcggacgg cggctgaacg gactctgccg aatgatctca
      181 gcggcacgcg ggtcaggcga aatgacggtc atcgccatgc gcttctcatt gaggatttca
      241 cgggcaactt cgataatgtc cgcctgggtg acggccatat acggctcgat ggccgaggtg
      301 ggattctgag gcggaaagtc gtagagaaac tcagtgacca ggcgattcat gcggttggtg
      361 gtactttcca tgccgatgag caggttgccg cgaatctgct gtttggcttc cgccagttcc
      421 gttttgttta gaccgtgctt agcgaccttt tcaacttcct tatgcacgag gtcgagcacc
      481 cgttgcagat gttcgagcga cgttcctgcg tagatggcaa agatgccact gtcgcggaat
      541 gcggtgtgat aggaatacac cgagtaggcc atgcctcgct tctcgcggat ctcctgaaac
      601 agccgactcg acattccgcc cccaaagacc gtgttcagca gggtcaacgc atagcggcgc
      661 gagtcgccat acgaggtgcc gggagcgcca agagcgacat gcacctgctc gatctctttc
      721 tgaaagatct tcacttcata acagggttca ggaatttctt ttcgagagcg gaagttgccg
      781 gttctcaacc cggacaaatg cttcgtaagc agtttcagca acctggtcca gtcaacattc
      841 ccggcggcgc agaccatcag gttttcggcg acatactgac gctcgtagat ctcgacgatc
      901 tgcgtgcgag tcataccccc gacggtcgcg gaggtgccaa gaatggggcg acccagaggg
      961 ctgttgtgcc acaggctcaa cccgagcagc tcatgaatca gctcgtcggg cgagtcttca
     1021 tacattttga tctcttcgag aatcacgccg cgctcgcgct cgagttcggt tttatcaaag
     1081 gtcgagttgc agaccatgtc ggccagcact tcaacggcct cgtcaaggtt gtccttgacg
     1141 acacgggcgt agtagcagca gtagtctttc gaggtgaagg cgttcaaatg cccgccgatg
     1201 cggtcgaact gccgggcgat gtctttgggc gaacgcttct tggtgccctt gaacagcatg
     1261 tgttcgatga aatgtgacgc gcccagtcgt tcgagctttt catagagcac gccgcttttg
     1321 aaaaagaaac ctatcgaaac gctctgcacg ttatcgaaag actcggcggc gacttgtact
     1381 ccattcgaga ggacgtcata cagaaaggga aacggttttt tcatgcgtgc ggttattctt
     1441 tccttggaag tacggcttct gatgtgtgtg atgcttggtc agtttcggtt tcagtctgag
     1501 gggcaccgga ctcagatgat gcgttcgcgg acgggtcatc accgcccgtc ggcacctgga
     1561 cacttccctc cggggtcgtt tccgtcgatg ccgggggtgc agaggccgcc accacgccct
     1621 cgacctccaa ctgcggggct tccgccgccg cggcttcttt cagcgcggcc ggatcaacat
     1681 ccacggctgg gacgggcagt tccttgagcg atttgatgcc aaagtagtag agaaagtcag
     1741 acgtcgtgga gtacaatctc ggttttccga taaccggctt ctcacccgag acatagatca
     1801 gcttcttttc gagaagcgtc gacatgacgc catcgcagtt gaccccgcga atcgcctcga
     1861 cctcggctcg cgtgatcggc tgtttatagg cgatgacgga cagggtctcg agcgcgggta
     1921 acgagagatt gaccagcctc tgaccttcga gtttttgaat gaacgtggca atggaagcct
     1981 tggtcgcgag ctgcacgccc tgctcgttga tcacgatctg cagaccgcgc tgcgggtcag
     2041 agtcatagcg ctccttgacc gcgagtatca gcggcatggc ctcttcgggg gcgacaccga
     2101 gggtctcgcc cagcagctgc acggaaagcg gttttccgtg aacgaacagc aggccttcga
     2161 gggccgcgta cagttgctgc cggcgctcct gcgcctcggt cagcgaaatg acattgtcgg
     2221 gaacggtctg agggacctga aggggttcgt cgattttcat gcggttgccg tcatttcttc
     2281 tgaagtatca tcggctgctt cttcgtcgcc atcaacagcc gcggcggcag ccggacgcag
     2341 atggatggga tgaaagttgc ctttctgggt cacacggagc tcaccaccct tggccatttc
     2401 gagcacagcc atcagcgaaa gtacgatctc atagcgattc tgcgggtctt tcagcagttc
     2461 tcggaaggtg gtcgcgcgct tgagggtggt cagatactcg cgaatctcct tgatgcgctc
     2521 ggccaggctc accttgtcga tgaccacccg atgcaccggc gggttgaggc ggcgacgcac
     2581 cagactctga aacaggttga gcagatcgac cgcgccaagc gagatctgca gaaactcctg
     2641 cggttgcggc tcgaagtcga taccgcgcgt gtaaatgcgc gagttctgcc gttcgagttc
     2701 gcgcatctgc atggccagca gcttgaaccg ccgatactcg agcagttttt cgaccagatc
     2761 gtggcgcgga tcgtcatcga actgcgcttc gtcgtagcct tcgtctccgg gctgcaggtc
     2821 gaccggtttt tccttgggca gcagcatctt cgacttgatc tcgagcagtg tcgccgccat
     2881 gaccagaaac tcggaggcga catccatgtc gaagatttcc atcaggtgca gatactcgag
     2941 gt
//
LOCUS       DAORHD010000564         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39181_length_2938_cov_2.823101, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000564 DAORHD010000000
VERSION     DAORHD010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39181_length_2938_cov_2.823101"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKO06_12175"
     CDS             complement(<1..292)
                     /locus_tag="PKO06_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV70448.1"
                     /translation="MSISRRSLVLRLSALALILVSLTWVHVTAVTAEDTAPVEVARKA
                     VVEGVPTEQPYNGCLLVRLDASTTSKILGFVKNGDVVVIEEMNGLWARISSPE"
     gene            complement(289..912)
                     /locus_tag="PKO06_12180"
     CDS             complement(289..912)
                     /locus_tag="PKO06_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV70449.1"
                     /translation="MPHRKRKSETPTYTYADYLTWGENQRCELIDGVVYSMTPSPSKT
                     HADISKALFRQLDLFFQGKSCQVYYAPLDVRLPKKDQTDENTDTVVQPDIIVVCDESK
                     LDEKGCRGAPDLVIEILSPSTASKDHITKKRLYETAGVKEYWLISPGDRIVTLYRRTA
                     RNGFGPAQIFGDTDIVSVSLFPGLEIDMKMLFPPLPPVVREPGVRYL"
     gene            complement(972..1892)
                     /locus_tag="PKO06_12185"
     CDS             complement(972..1892)
                     /locus_tag="PKO06_12185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HNV70450.1"
                     /translation="MQTYSADSRLANDPLQFARRHLEEGRPKAEIEAIALYSAMLSYG
                     KVDLFLAVIRQTLDLAHDRFLDFVTAHPFSQHQVSQISPGRSRTQEPAGLVESDRQSI
                     AQTVSTPAGFPRYRLSTADEIWHFARAIGWVVRVDGGLFPSFYAGFVPQRSLREGLVS
                     LRNRLLDGLHAHHVDLSPGLAHLLPDPALGGACKRWLMFLRWVVRPDDGLDLGLWREI
                     PAAVLLIPIDRHVSAIARALGLTQRKTDDWKTAEEITTALRRFAPDDPVRYDFALAHL
                     GISGECTHGRDPAACRTCRLRTLCTHGTMR"
     gene            complement(2045..2527)
                     /locus_tag="PKO06_12190"
     CDS             complement(2045..2527)
                     /locus_tag="PKO06_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70451.1"
                     /translation="MMRQFNLIRCAVLWALGGVMLCQFAFTAPLPGDPAFVKRFIAVT
                     EKLAELETSESPNASKLIEQLNAQVSWLVSAFEKNPDLLVTYLQAGYRPDNLLYSKLR
                     ERFAFAISQESQKRLQPAFEMLEHVRRTRPSSHPSPARSRSSARCICSKPSFRASQRN
                     "
     gene            complement(2668..>2938)
                     /locus_tag="PKO06_12195"
     CDS             complement(2668..>2938)
                     /locus_tag="PKO06_12195"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNV70452.1"
                     /translation="TVYRTLWTLIDLGLLTNLGPDQRRVRFDANLDHHHHFICMNCGK
                     TIDFHAPTFDRLHPPTSVTRLGKIERVQVECRGVCKACSSRARNR"
BASE COUNT          627 a          796 c          939 g          576 t
ORIGIN      
        1 tctcgggtga cgaaatgcgc gcccagaggc cgttcatctc ttcgataacc acgacgtcgc
       61 cgttcttcac gaagccgagt atcttcgagg tggtactggc atcgagacgt accagcagac
      121 agccgttgta gggctgttcc gtcggcactc cttcaacaac ggctttgcga gctacttcca
      181 cgggcgctgt gtcttcggcg gtaaccgcgg tgacgtgaac ccaggtcagt gagaccagta
      241 tcagcgccag agccgaaaga cgaaggacaa gggaacgacg cgagatactc ataaatatcg
      301 gactcccggt tcccgcacca cgggcggtaa cggcggaaac agcattttca tgtcgatctc
      361 gagaccggga aacagagaca cggatacgat gtcggtgtcg ccaaagatct gggccggacc
      421 gaacccgttg cgggccgtgc gccggtagag cgtgacaatg cggtcgccgg gtgagatcag
      481 ccagtattct ttcactccgg cagtctcata gagccgcttc ttcgtgatgt gatctttcga
      541 agccgtcgag ggtgagagaa tctcgatcac cagatcggga gcaccccgac aacccttttc
      601 gtccagtttg ctctcgtcgc agacgacgat gatatcaggc tgaacgacgg tatcggtgtt
      661 ctcgtcggtt tgatctttct tcggcagccg cacgtcaagg ggggcgtagt aaacctggca
      721 tgattttccc tggaaaaaca gatcgagttg tcgaaacagt gctttcgaga tgtccgcgtg
      781 ggttttcgac ggtgaaggag tcatggaata gacgactccg tcgatcagtt cacagcgctg
      841 gttctcgccc caggtaagat agtcggcgta cgtgtaggtc ggcgtctcgg atttccgttt
      901 gcggtgcggc atgtgtcacc ctccacagat cagcgaaagt tcaccagccc agtctatcac
      961 aacgccgctc gtcagcgcat cgtgccgtgg gtgcagagcg tgcgcaggcg gcaggtgcgg
     1021 caggccgcgg ggtcgcggcc gtgcgtgcat tcgccgctga tgccgaggtg agcgagggcg
     1081 aagtcatagc gaacggggtc atccggggcg aagcgacgaa gggcggtggt gatctcttcg
     1141 gcggtcttcc agtcatctgt cttgcgctga gtcagaccga gtgcgcgggc gatcgcactg
     1201 acgtgccggt cgatgggaat gagcaggacg gcggcgggaa tctcacgcca caggccgaga
     1261 tcgaggccgt cgtcgggacg caccacccag cgcaggaaca tgagccaccg tttgcaggca
     1321 ccgcccagag ccgggtcagg cagcagatgt gccaggcccg gcgaaagatc gacgtggtga
     1381 gcgtgtagcc cgtcgaggag ccggttccgc aaagagacaa gaccttcgcg cagagaacgc
     1441 tgcggaacaa aaccagcata aaaagaggga aacaacccac catcgactcg gacaacccaa
     1501 ccgattgctc gggcaaagtg ccagatctcg tcggccgtac tcagacgata cctggggaag
     1561 ccggcaggcg tgctgacggt ttgggcaatg ctctgtcggt cggactctac gagacccgcc
     1621 ggttcttgtg ttcgcgaacg tcctggtgat atctggctga cttgatgttg agaaaaggga
     1681 tgagcggtga cgaaatcgag aaatcgatca tgtgcgagat cgagtgtctg gcgaatcacg
     1741 gcgagaaaca gatcgacctt gccgtaggac agcatcgcgc tgtacaaggc tattgcctcg
     1801 atctcggctt ttggtcggcc ttcttcgaga tgtcgacgcg caaactgcaa cgggtcgttg
     1861 gcgagccggg aatctgcaga atacgtttgc accagtcttt caagctctcg ttgaagcgtg
     1921 ctgataggtg gtcgcattgg tgcctgagaa gacggcggtc gctggcgacg ggctcgcgaa
     1981 gtcacagtct caccattgtg tgggcgaacc aggaaccagg aaaatcactc ttcggcttcg
     2041 acgatcagtt tcgctgactt gcccgaaagc tcggttttga gcagatgcag cgagcactcg
     2101 agcgggatcg tgccggactc ggatgcgacg acggtcgagt gcgacgcacg tgctcaagca
     2161 tctcgaacgc cggctgcaga cgcttctgcg actcctggct gatggcgaag gcaaaccttt
     2221 cgcgaagctt cgaatacaac aggttgtcgg ggcgatagcc ggcttgcagg taagtgacca
     2281 gaagatccgg gttcttttcg aacgcactga cgagccaact cacctgcgca ttcagttgtt
     2341 cgatcagctt cgaggcgttc ggcgactcgg acgtttcgag ttccgccagc ttctcggtca
     2401 cagcgatgaa tctcttgacg aaggccgggt caccgggcag cggagccgtg aacgcgaatt
     2461 gacaaagcat cacgccgcca agtgcccaga gaaccgcaca ccgaatcagg ttgaattgtc
     2521 tcatcatacg tcgtgcccct tgccgccttc tgattgtctc gccaatcact gagcattatc
     2581 tcacgaccgc gttgtgccac acaacaccgc tgggttcagg cagtattcag aagaaagaac
     2641 gagagaggaa tgcgggggag gggcgcgtca gcgattgcgg gcgcgagagg aacaggcttt
     2701 gcagacgccg cggcattcca cctggacgcg ttcgattttt cccaggcggg tcaccgaggt
     2761 tggaggatgc aggcgatcga aggtgggcgc gtgaaaatca atggtcttgc cgcagttcat
     2821 gcagatgaag tggtggtggt ggtcgaggtt ggcgtcgaat cgcacgcgtc gctgatcggg
     2881 gccgagattg gtcagcagac cgagatcgat cagggtccag agcgtgcggt agacggtg
//
LOCUS       DAORHD010000565         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39211_length_2936_cov_3.428671, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000565 DAORHD010000000
VERSION     DAORHD010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39211_length_2936_cov_3.428671"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PKO06_12200"
     CDS             complement(<1..166)
                     /locus_tag="PKO06_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNV70453.1"
                     /translation="MATIVPRPETGLIIGKFLPPHRGHQFLIDFARRHCRKLTVLVCT
                     LQREPIPGRLR"
     gene            complement(130..1680)
                     /gene="pnuC"
                     /locus_tag="PKO06_12205"
     CDS             complement(130..1680)
                     /gene="pnuC"
                     /locus_tag="PKO06_12205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNV70454.1"
                     /translation="MLLRVIDILTWEEIFGLIVMGSLIVGPPVLFLGRLVECWRGSAA
                     ASRRRTFFWGMATVIVALCALYGYFLEPRWVEYRTIRLPIRGVPADRVVRLVHLTDFH
                     YETRALGWPGEQVLARIASMMPDLICLTGDYLNDAGEAAAFRLWLEKLRQMAPTYAVL
                     GNWDFHMTRGGQLVKDVGIPILDGSWVDVAPASVPLRLLGIPLWSEMPGVESGAFQTD
                     QPVVVLYHTPDRFPDAAAASAALYLAGHTHGGQVCLPLYGPVVTLARTGRKYASGLYK
                     SGDTWGFTGRGLGMEGGHAPRIRFCARPQVTLIELAAPGTTTPPEPDGVADPVELLAV
                     ILGFINVWLIIRQSVWCWPPGLLSTGLYVFIFYQARLYSETILQLLFIVLQLHGWYYW
                     VFGGRRSDEASVPVTRLTVAQRVKWAVGTLILAAALGTAMKTWTNADLPYLDALVTAM
                     SISAQILMNVKVLESWLLWITVDIFSIGIYYVKGLTFTLGLYTAFLIMATMGYFAWRR
                     SFLDQKPV"
     gene            complement(1770..>2936)
                     /locus_tag="PKO06_12210"
     CDS             complement(1770..>2936)
                     /locus_tag="PKO06_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70455.1"
                     /translation="QVPGVRALTDQDPGFNVQHLEDRASVIFWRFTTANRIGNLAPLR
                     KMCCESCADKFKTDVDFNAQGVRRFYGNCAVGSVDVRGIAPADDFDRAYIEIRWSGTP
                     LEIKTSEKQPREIGDVMNFHHGFILRRKHETKTDVRNALVSSHCPNCGGPEGNTDAPA
                     CEYCGTVVNDGSFDWVLEQIVPVGSPEFRDKLQEMRRTTAQVTKPSRPGAAVSPTSVP
                     PQPVQTPGGPGAFLELPGTGPVIEAPPPGGAPDVATGALFGMAGMDCVRWVVQMMLAD
                     GVIEDKEREYLKEFAARCGVAAARLEGMISAVAAGAADLPVPDDRSEARELLKAIAGM
                     ALADGRLSPEEASILNAFGGRIGLSPFDVNMLVKQERQRLYQVAQQALRTKKNV"
BASE COUNT          654 a          938 c          852 g          492 t
ORIGIN      
        1 agcgcaagcg cccgggaata ggctcacgtt gcagcgtaca gaccagcacc gtcagctttc
       61 gacagtgccg gcgggcgaaa tcgatcagaa actggtgccc gcgatgcggc ggcagaaact
      121 tgccgatgat cagaccggtt tctggtcgag gaacgatcgt cgccatgcaa aataccccat
      181 cgtggccatg atgagaaacg ccgtgtacaa acccagagta aaggtcaggc ccttcacata
      241 atagatgccg atgctgaaga tgtctaccgt gatccagagc agccagcttt cgagcacttt
      301 cacgttcatc aggatctggg ccgagatgct catggcggtc accagggcat cgagatacgg
      361 caggtcggcg ttcgtccagg ttttcatagc cgttcccaga gccgctgcga gaatcagggt
      421 gccgaccgcc cacttcacgc gttgtgcgac cgtcagacgc gtgacgggaa ccgatgcctc
      481 gtcagacctg cgcccgccaa aaacccaata gtaccagcca tgcagctgca acacgatgaa
      541 tagaagctgg agaatggtct ctgaatagag acgagcctga tagaagatga agacatagag
      601 cccggtcgac agcagcccgg gaggccagca ccagacactc tgccgaatga tcagccagac
      661 attgatgaac ccgagaatca cggccagcag ttcaaccgga tcagcgacgc catcaggctc
      721 gggcggggtg gtggttccgg gggcagccag ctcgatgaga gtcacctgcg gacgcgcaca
      781 gaacctgatg cgaggagcat gacctccttc catgcccaga ccacggccgg tgaatcccca
      841 ggtgtcgccc gacttgtaca gccccgaggc atacttccga ccagtgcggg ccagggttac
      901 gaccgggccg tacagcggca ggcagacctg tccgccatgt gtgtgtccgg ccagatacaa
      961 tgcggcactg gcggctgccg catccggaaa ccggtcggga gtgtgataca gcacgaccac
     1021 aggttggtca gtctgaaatg ctcctgactc aacgcccggc atctctgacc agagcggaat
     1081 accgagcagt cgcagcggca cggaggcagg agcgacatcg acccagcttc cgtccagaat
     1141 cggaatgccg acatctttga ccaactgacc gccacgagtc atgtgaaaat cccagtttcc
     1201 gagaacggca taggtcggag ccatctggcg cagcttctcg agccagagcc gaaacgccgc
     1261 cgcttcgcca gcatcgttca ggtagtcacc ggtcaggcag atcagatcgg gcatcatcga
     1321 ggcaatacgc gccagcacct gctcgcccgg ccagccgagt gctcgcgttt catagtgaaa
     1381 atcggtcaga tgcaccagac gcacaacacg gtcggctggc actcccctga tcggcaggcg
     1441 aatcgtgcga tactccaccc agcgcggttc cagaaaataa ccgtacagag cacacaacgc
     1501 gacgatgacc gtcgccatac cccagaagaa cgtgcgccgt cgcgaggccg ccgcggatcc
     1561 acgccagcat tccaccagtc ggccgagaaa cagcaccggc ggaccgacaa tcagggaccc
     1621 catgacgatc aacccgaaaa tttcttccca ggtcagaatg tcgatcacgc gcagcagcat
     1681 gtcaagccac gcacaagggt tcgaaggcag gcacggccat cagacgagcc gatcggcgac
     1741 agaccgcacc ccgcgcaaca ttcaacatct cacacattct tcttggtgcg tagagcttgt
     1801 tgagccacct ggtacaaacg ttggcgttcc tgtttcacca gcatgttcac gtcgaaggga
     1861 gaaagtccga tacggccgcc aaacgcgttc aggatggacg cttcttccgg cgagagtcga
     1921 ccatccgcca acgccatgcc ggcaatcgcc ttcagcaatt cacgagcctc ggaacgatcg
     1981 tcaggaacgg gaagatcggc ggccccggcc gccaccgcgc tgatcatgcc ttccagccgg
     2041 gcggcggcaa caccacaacg agccgcgaac tccttgagat actcccgttc cttgtcctcg
     2101 atgaccccat ccgcgagcat catttgtact acccagcgca cgcaatccat gcctgccatg
     2161 ccgaacagag caccggtcgc cacatcggga gcaccaccgg ggggcggcgc ctcgatcacc
     2221 ggtccggttc cggggagctc cagaaaggct ccgggcccac ccggggtttg aaccggttgg
     2281 ggcggcacgc tggtcggaga aacggcggca cccggacgcg aaggcttcgt gacctgcgca
     2341 gtcgttcgcc gcatttcctg cagtttatca cggaattccg gagaaccgac cggtacgatc
     2401 tgctcaagca cccagtcgaa ggacccgtcg ttcaccaccg tgccgcagta ttcacaggcc
     2461 ggcgcatcgg tgttcccttc cggaccgccg cagttcggac agtgcgagct gaccagggca
     2521 ttgcgcacgt cggtcttcgt ctcgtgtttg cggcgcagaa tgaacccgtg atggaagttc
     2581 atgacgtcac cgatctcacg cggctgcttt tccgacgtct tgatttccag tggtgtgccg
     2641 ctccagcgga tctcgatgta ggcacggtcg aaatcatcag ccggagcgat gccacgcaca
     2701 tcgaccgagc cgaccgcgca gttgccgtag aaccgtcgca cgccctgcgc gttgaagtcg
     2761 acatcggtct tgaacttgtc cgcgcagctc tcgcagcaca tcttgcgcaa tggagcgagg
     2821 ttcccgatgc ggttggcggt agtgaagcgc cagaagatca ccgaggctcg atcttcgaga
     2881 tgctgcacgt tgaagccggg gtcttgatcg gtcagagccc gcactcccgg cacctg
//
LOCUS       DAORHD010000566         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39336_length_2930_cov_3.181217, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000566 DAORHD010000000
VERSION     DAORHD010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39336_length_2930_cov_3.181217"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..2060
                     /locus_tag="PKO06_12215"
     CDS             72..2060
                     /locus_tag="PKO06_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV70456.1"
                     /translation="MSLRARQILLPALVAALLLHYLPEPLVEARSKTRPANSIGTWQS
                     LKRSPAASIQPPVSPGTPGALPTIRQPANGFQRPTSRSMDWYTLGDSAKIRMGIPGQK
                     LPLPMRAESFDALSQPIPVPRFPLAQLPASAPTPTPTSPSPVSPVTGAVPQGLVPPTT
                     APARGEMQLDTEFRLPEGMNIRIRSMDFRDTDVRDVLRSLSALVNLNLMLSNNISGAI
                     TIMFNDVTMEEAFSTVMKNADLGFTWDRTILRIFPMAQAPTVTQVFNIQNANAAAIKP
                     MVDRILTSGRQSEIDARTNSLIVTDTQAKIDQVRLLLPQIDIQSTAVEVTSRPITEVF
                     YLDYVDANNMTTPIRSVAPDANITAYSSTQASQAGAGGTATGRQDIMIITDTQANIDK
                     IREIIEKLDVAPVQVTIDAHIYEIDLNEEERMGINWQKSIPIPGTTENIFDMSISPEE
                     ATAGGTGVFRFGSLNVNQFRALLAMLKTKTFAKVLSNPVITTLNNRQANITVGQAIPY
                     ISASTVNAETGQVSNTVSQANANIQLQVTPSVTGNDEVFLDITPTISSVLGFTSLGGN
                     STPNLSNRTAQTQVIVKNNHTIVIGGMIKTDKSDTLSKVPILGDLPGIGRLFQKKTLK
                     EIRTELIIFITPHIVRNHGGQKRHPQLEAQLPKLSVQP"
     gene            complement(2065..>2930)
                     /locus_tag="PKO06_12220"
     CDS             complement(2065..>2930)
                     /locus_tag="PKO06_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70457.1"
                     /translation="ELILDNPSGLRLGGSKSEVTMFFSDIRGFTKMSESMDPTAIVEL
                     LNEYFTRMTDILMELDGTLDKYVGDEMMALFGAPVAQPSDPIRAVLCAVKMMGALREL
                     QEKWKKEGKPIINIGIGINTGEVTAGYMGSEKQLSYTVIGDNVNLAARVMANAKPMQV
                     LITKVVWDRVQEYFLADPLEPIMVKGKSKPIEIYEVKGVRDGVDFQKVIGMDVGKLVS
                     LGAASSPTAPVSGVPQKQSSAPLNISAEEAKKVNIVIDPKANKAIECQNCGTENEAQN
                     KFCSKCGMPIF"
BASE COUNT          676 a          881 c          730 g          643 t
ORIGIN      
        1 acgccgttca cgattaggca gggaatgccg ataagagaag ggaattcgtg ttttcggtcg
       61 aggagaacca gatgtccctg cgtgctcggc agattctgtt gcccgccctg gtggcggcgc
      121 tattgctgca ttatctgcct gagcctttgg tcgaagcccg ttcgaaaacc cggccggcca
      181 actcaatcgg cacgtggcaa tcactcaaac gatcgcccgc tgcctctatt caaccgcccg
      241 tttctccggg aactccgggc gcgttgccca caattcgtca gccagcgaac ggttttcaga
      301 ggccaacctc gcgcagcatg gactggtaca ctctcggtga cagcgccaag atccgcatgg
      361 ggattccggg gcagaaactt cccctgccca tgcgggccga atcattcgac gctctgtcgc
      421 aaccaatccc ggtgccgcgc tttcctctcg cacaacttcc cgcctctgcg cccaccccta
      481 ccccaacatc gcccagtccg gtctcaccgg taacgggagc tgttccgcaa gggttggtac
      541 ctccgaccac tgcaccagcc cgcggtgaaa tgcagctcga cacggagttc cgtttgcctg
      601 aaggcatgaa cattcgcatc cgatccatgg actttcgtga cacggatgtt cgtgatgtac
      661 tgcgctctct gtcagcgctc gtcaacctga atctgatgct ctcgaacaac atcagcgggg
      721 ccatcaccat catgttcaat gatgtcacga tggaagaagc gttctcaacc gtcatgaaaa
      781 atgccgatct cggtttcacc tgggatcgaa ccattttgcg catctttccg atggcgcaag
      841 cccccaccgt cactcaagtg ttcaacattc agaatgccaa cgcggcagcg atcaagccaa
      901 tggtcgatcg aattctgacg tcggggcgac agtccgaaat cgacgcgcgc accaattctc
      961 tgatcgttac agatactcag gcaaagatcg atcaggtgcg tctgctcttg cctcagatcg
     1021 atatccagtc gacggcagtc gaagtgacct cgcgcccaat cacggaagtc ttctatctgg
     1081 actatgtcga cgcgaacaac atgacgacgc ccattcgctc ggtcgccccc gatgcgaaca
     1141 tcaccgcgta ttcctcaaca caggcttcac aggccggcgc aggtggtacc gccaccggac
     1201 gtcaggatat catgatcatc acggacaccc aggccaacat tgacaagatt cgagaaatca
     1261 tcgagaagct ggatgtcgct ccggtacaag tcactattga cgcccacatt tacgagatcg
     1321 atctgaacga agaagaacgc atgggaatca actggcagaa aagtattccg attcccggaa
     1381 cgacggaaaa catctttgac atgagcattt cgcctgaaga ggcgacggct ggtggaacgg
     1441 gagtctttcg tttcggcagc ttgaacgtga atcagttcag ggctctgctg gccatgctga
     1501 agacaaaaac gttcgccaaa gtgctcagca acccagtcat tacaacgttg aacaatcgcc
     1561 aggccaacat cactgtcggc caggccattc cgtacatctc tgcctcgacc gtcaatgccg
     1621 agaccggaca ggtttcaaac acggtcagtc aggccaatgc gaacattcaa ctccaggtca
     1681 ctccatccgt gaccgggaat gacgaagtat ttctggatat cacgccgacc atttcttcgg
     1741 ttctcgggtt cacctcactg ggcgggaact ccactcccaa cctcagcaac cgtacagcac
     1801 agactcaggt catcgtgaaa aacaatcaca caatcgtcat tggcggtatg atcaaaactg
     1861 acaaaagcga caccctctcg aaagttccca ttctcggcga tctgcccgga atcggcaggt
     1921 tgtttcagaa aaagaccctg aaagagattc gcaccgagtt gatcattttc atcacgccgc
     1981 atattgtccg aaatcatggc gggcagaaac ggcatccaca gctcgaagca cagctaccga
     2041 aactcagcgt acaaccctga ccgatcagaa gatcggcatg ccgcacttac tgcagaactt
     2101 gttctgcgct tcattttcgg tgccacagtt ctgacactcg atcgccttgt tggctttcgg
     2161 gtcaatcacg atgttcactt tcttcgcttc ttccgctgag atgttgagtg gcgcggagct
     2221 ttgtttctga ggaactccgc tgacgggggc ggtcggggaa gacgcggcac cgagcgagac
     2281 cagcttgccg acatccatac cgatgacctt ctggaaatct actccgtccc tcacgccctt
     2341 cacttcatag atctcgatgg gcttcgactt tcctttcacc atgatgggtt cgagcggatc
     2401 agcgaggaag tattcctgca cgcggtccca gacgactttc gtgatcaaca cctgcatggg
     2461 cttggcattt gccatgacac gggcggcaag gttcacgttg tcgccgataa cggtgtagga
     2521 aagctgcttt tcgctgccca tgtacccggc cgtcacttca cccgtgttga tgccaatacc
     2581 gatgttaatg atcggctttc cttccttctt ccacttttcc tgcagttcgc gcagagcccc
     2641 catcattttt acggcacaga gaaccgcccg aatcgggtcg ctgggctgcg ccaccggagc
     2701 gccgaaaaga gccatcattt catcaccgac gtatttatcg agcgtgccat cgagctccat
     2761 gagaatgtcg gtcatgcggg taaagtactc gttgagcagt tcgacaatgg ccgtcggatc
     2821 catgctttcg gacatcttgg tgaaaccgcg aatatccgag aagaacatgg tgacttctga
     2881 tttactcccg cccaggcgca gcccgctggg gttatcaaga ataagctcgg
//
LOCUS       DAORHD010000567         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39556_length_2919_cov_3.894902, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000567 DAORHD010000000
VERSION     DAORHD010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39556_length_2919_cov_3.894902"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2089
                     /locus_tag="PKO06_12225"
     CDS             <1..2089
                     /locus_tag="PKO06_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HNV70458.1"
                     /translation="YNNRTLRIDLTTLTITEEPVDDKMKEIFIGGRGFGLWRLWNSVK
                     PTSTWRSPENTIVISPGPVAGNTQYPGSGKSLVVTLSPLTDIPIDSNVGGYYGPLLKF
                     SGFDAIEITGKAKTDVVVVVDGQKGTITIEEAPLEEVNSHVAAEQFIHEYANDEADMR
                     NVSTVTVGRGGDNCRIGCLNFSWFDVRRKAARLKQAGRGGIGTVFRDKKVKALVVHGP
                     PIKGDMNQPVNHDKVAKAGMRLHREIYENDDKQCHMRRQGTAHLVEIMDAYDLLPVHN
                     FQYGKHPDTPKIASWVWDHRFTQGIPDGCWYGCSMACSKGADGHIVMTGPYKGHVVTV
                     DGPEYETAAGVGANCGIFDPNWVLECNFYCDTYGIDTISFGTSCSFVMECYQRGILNA
                     ERTGGLELTWGNGAAQMELMHQMARGEGFGKIAGQGVMRMKRIFAAKGWGDAQFLDDI
                     GMENKGLEYSQYVSKESLAQQGGYALTNKGPQHDEAWLIFMDMVNKQIPTFEAKAEAL
                     HYFPLWRTWFGLNGLCKLPWNDVEPPDNQRVNDPMEAAKVPEHIQNYVDIFTGITGRQ
                     ASREDLITMSQKVYNFQRVFNIRMGKGLRKYDKAPYRSKGPVTVEEYESRAERYDKQI
                     QEVLKVDPRGKSTADKVALLRQYREDQYEKLTDAVYQRRGWTKDGVPTLETLKKLGID
                     FPEIVEVVKPLLG"
     gene            2273..2905
                     /gene="thiF"
                     /locus_tag="PKO06_12230"
     CDS             2273..2905
                     /gene="thiF"
                     /locus_tag="PKO06_12230"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HNV70459.1"
                     /translation="MKIYINEREFDVSDNCTVSDLRSIHVPRADVIVQNGFPANPDDP
                     VRENDRLIFIRKGEVPPPDELEALMAARHTPGVHARLKRASVGIAGCGGLGSNAATAL
                     ARVGIGHLVLVDFDVVEPSNLNRQAYFVDQIGLPKVQALKANLKRINPAVRIDAHDCL
                     LTPQNISALLGGVDVIVEAFDRADQKSMLGETCAGLLPDKPAQVSPSIDF"
BASE COUNT          674 a          844 c          827 g          574 t
ORIGIN      
        1 ctacaacaat cgcactctgc gcatcgatct gaccacgttg acgattacag aagagcccgt
       61 cgacgacaag atgaaagaga ttttcatcgg cggacgcggg tttgggctct ggcgcctctg
      121 gaactcggtg aaaccaacga gtacctggcg atcaccggaa aacacgattg tgatcagccc
      181 cgggccggtc gctggtaaca cccagtatcc cggctctggg aaatcgctgg tcgtcaccct
      241 gtctccgctc accgatattc ccatcgattc gaatgtcggc gggtattacg gcccattgct
      301 gaagttttcg ggtttcgatg ccatcgagat cacaggcaaa gccaagaccg acgtagtcgt
      361 ggtggtcgat ggtcagaaag gcaccatcac catcgaagaa gctccactcg aagaagtgaa
      421 cagccacgtg gccgctgaac agttcattca cgagtatgcc aatgatgaag ctgacatgcg
      481 caacgtgtcg acggtcacgg tcggacgcgg cggcgacaac tgccgcatcg gctgtctgaa
      541 cttctcgtgg ttcgacgttc gccgcaaagc ggctcgcctc aagcaggccg ggcgcggcgg
      601 tatcggcacg gtgtttcgtg acaagaaggt gaaagcgctg gtcgtgcacg gaccgcccat
      661 caagggcgac atgaatcagc ccgtgaacca tgataaggtc gccaaagccg gcatgcgcct
      721 gcatcgcgag atttatgaaa acgatgacaa acagtgtcat atgcgccggc agggaaccgc
      781 tcacctggtc gaaatcatgg atgcctacga cctgctgccg gttcacaact tccagtatgg
      841 caaacacccc gacacaccca agatcgccag ctgggtttgg gatcaccgct tcacgcaggg
      901 gattcccgac ggttgctggt atggatgttc gatggcctgt tccaagggcg cagacggtca
      961 tatcgtcatg accggcccgt acaaaggaca tgttgtcacc gtcgacggcc ccgaatacga
     1021 aaccgcggcc ggcgtgggcg ccaattgcgg catcttcgac ccgaactggg tgctcgagtg
     1081 caacttctac tgcgacacct acggcatcga caccatctcg ttcggcacct cctgttcctt
     1141 cgtcatggaa tgctatcagc gcggcattct gaacgcagaa cgtaccggcg gccttgagct
     1201 cacgtgggga aacggtgcgg ctcagatgga gctgatgcat cagatggctc gcggagaagg
     1261 attcggaaaa atcgccggtc agggcgtcat gcgcatgaaa cgcattttcg ccgcgaaagg
     1321 gtggggtgac gcccagttcc tcgatgacat cggcatggaa aacaagggcc tcgagtactc
     1381 gcagtatgtt tcgaaggaat cgctcgccca gcagggtggc tacgcgctga ccaacaaagg
     1441 tccgcaacac gatgaagcct ggctgatctt catggatatg gtcaacaagc agattccgac
     1501 gttcgaggcc aaagccgagg ccctgcacta cttcccgctg tggcgcacct ggttcgggtt
     1561 gaacggcttg tgcaagctgc cctggaacga tgtcgaacca cccgacaatc aacgcgtcaa
     1621 tgatccgatg gaagccgcca aggtaccaga acacatccag aactatgtcg acatcttcac
     1681 cggcattacg ggacgtcagg ccagtcgcga agatctgatc accatgtcgc agaaggtcta
     1741 caactttcag cgtgtcttca atattcgcat ggggaaagga ctgcgcaagt atgacaaggc
     1801 gccgtatcga tcgaaaggac cggtcactgt cgaagaatac gaatctcgcg ccgaacgcta
     1861 tgacaagcag atccaggaag tgttgaaagt ggatccgcgc gggaaatcga cagccgacaa
     1921 agttgccttg ttacgacagt accgcgaaga ccagtatgag aaactgaccg acgccgtcta
     1981 ccagcgccgc ggctggacca aagacggcgt accaactctc gagacactga aaaagctcgg
     2041 catcgacttt cccgagatcg tagaagtggt aaagccgctg ctcggataac cgcgccaacc
     2101 ttgcgtgaat cgccctgttc gtcgatgacg gacagggtga ttctttttcg cggagtcgaa
     2161 aactgatcac cgcgttgagc cgccttctca atcgcctgga acgacttttc caggacttcc
     2221 aaacgtggcg gagttccctc agaaacacgc gaatggtata cttgatttgg ttatgaagat
     2281 ttatatcaac gaacgtgaat tcgacgtttc tgacaactgt accgtttctg atcttcggtc
     2341 gatccatgta ccgcgcgccg atgtcatcgt ccagaacggg tttccggcga atcctgacga
     2401 cccggttcgc gagaacgacc gactgatctt tatccgtaag ggcgaagtgc ctcctcccga
     2461 cgaactcgag gccctgatgg ccgcacgaca caccccggga gttcatgcgc gcctgaagcg
     2521 ggcttccgtg ggtattgccg gatgcggtgg tctggggtca aacgcagcta ccgcgctcgc
     2581 gcgtgtgggt atcgggcacc tcgtgctcgt tgatttcgac gtggtcgagc cttcgaacct
     2641 gaatcgccag gcgtatttcg ttgatcagat cggcctgccg aaggtgcagg ccctgaaagc
     2701 caatctcaag cgcatcaacc ccgcagtacg cattgacgcc cacgactgcc tgctcacccc
     2761 gcagaacatc agcgcactgc tgggcggagt cgacgtcatc gtcgaggcct tcgatcgcgc
     2821 cgatcagaaa tcgatgctcg gggaaacctg tgccggtctg cttcccgaca aaccggcaca
     2881 ggtttccccg agcatcgatt tctgatcggc gcgatcgaa
//
LOCUS       DAORHD010000568         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_39807_length_2908_cov_3.703470, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000568 DAORHD010000000
VERSION     DAORHD010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39807_length_2908_cov_3.703470"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="PKO06_12235"
     CDS             complement(<1..1423)
                     /locus_tag="PKO06_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70460.1"
                     /translation="MHQVSLDYGALFDLVDMGVFVVEPETARLLDANACALAMTRCTM
                     DELRNLPLEHFLTSEPGYSVADAVTWVRKAASEGRQKLRWQGRDLQGGIYWVDVVLQA
                     VTTGTQTVILALCRNATHDRAQEQSLFEAEARYRAIVEAFDGLIYICSGDYQVEYMNQ
                     HFIDRTGRSAIGELCYRALHGRDTICPWCVNDRVQAGETVRWEVKSPLDNRWYYIVNT
                     PLRHADGRISKQALIIDVTDRRQAEEQRRALEQKMRETQRFESLGHLAGGVAHDFNNL
                     LLVMLGQMDLVLNSLPADSALRENLQEGINAAQHASGLCRQMLTYAGKNVFKPQVIDL
                     NVHVRDVESLLRLSVSRQAELVLDLSSDSARMSGDPSQLQQVIMNLVINASESLEGRT
                     GRIVVRTGSGRFTPDELACRWLHQPPAVGDYAWLEVSDTGIGIPEEHFPHLFDPFFST
                     KFTGRGLGLAAVLGIVRGHQGSIQ"
     gene            complement(1473..2627)
                     /locus_tag="PKO06_12240"
     CDS             complement(1473..2627)
                     /locus_tag="PKO06_12240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV70461.1"
                     /translation="MKPRILLVDDEPNILDSYGRILGRQFHLFTALSGEAGLKLIDEQ
                     GPFAVIISDFNMPRMDGIRFLSLAREKAPETVRMMLTGEGDFQIAIKAVNEGSVFRFL
                     TKPCSQPDLLRALEAGVEQYRLIQAEKEARRQEIRIAGEIQQTLLFETPPTQIHGAQI
                     ACFTQASAGVDGDFLDFFVHDNTCFDLILGDVMGKGIPAALVGAGTKNRFSRVLAQLL
                     RNLGEAALPEPEAVMTRVQSEAGNHLASLGRFVTLCYARFNTQTRTMSFVDAGHPPAL
                     WSTPQGAVQLKGEGFPLGFPESRDYQQTTVSFEPDDVFVFYSDGLMDGRDANGAFFGI
                     ERLQELIHGLRSEPAHAILTSIIGTYRTFVSSDSFTDDLTCVVVKSTRTE"
     gene            complement(2624..>2908)
                     /locus_tag="PKO06_12245"
     CDS             complement(2624..>2908)
                     /locus_tag="PKO06_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70462.1"
                     /translation="FETSHAEIGGYLLGIWGLPDVTVLTVACHHRPLQIPPEGFSPLT
                     AIHVANALLETRAGRPTKPDSEYLSFLGLTDHLRSWEILADHAWERSKRR"
BASE COUNT          612 a          827 c          880 g          589 t
ORIGIN      
        1 cctgtattga tccctgatgg ccgcggacga tccccagcac ggcagccaga ccgagaccgc
       61 gcccggtaaa cttggtcgag aagaacggat cgaacaggtg cggaaaatgt tcttcgggaa
      121 tcccgatacc ggtatcgctg acctcgagcc aggcatagtc cccgacggcg ggtggctgat
      181 gcagccagcg acaggccagt tcgtcgggag taaaacgtcc tgacccggtt cgcacgacga
      241 tgcggcccgt gcgaccttcg agtgactctg aggcgttgat caccagattc atgatgacct
      301 gctgcagctg cgacgggtcg ccggacatgc gtgccgagtc actgctgaga tcgagcacaa
      361 gttcagcctg ccttgaaacc gacaatcgaa gcagactttc gacgtcgcgc acatggacat
      421 tgagatcgat aacctggggt ttgaacacgt tcttcccggc atatgtcaac atctgtcgac
      481 agagacccga ggcgtgttga gccgcgttga tgccttcctg caggttttcg cgcaacgcgg
      541 aatcggccgg caggctattc aggacgaggt ccatttgtcc cagcatcacg agcagcagat
      601 tattgaaatc atgagcgact ccgccggcca gatgtccgag actctcgaaa cgctgcgttt
      661 cgcgcatctt ctgttccagg gcacgtcgtt gttcctcggc ctggcgtcga tcggtgacat
      721 cgatgatcag ggcctgtttc gagatgcgtc catcggcgtg ccgcagaggg gtgttgacaa
      781 tgtaatacca gcggttgtcg agcgggctct tgacctccca gcgaacggtt tcccccgcct
      841 ggacgcggtc gttgacgcac caggggcaaa tggtatcgcg accatgaaga gcgcgatagc
      901 agagctctcc gatcgccgac cgccctgtac gatcgatgaa gtgctgattc atgtattcga
      961 cctgatagtc accggaacag atgtagatca acccatcgaa agcctcgacg atggcgcgat
     1021 accgggcttc agcttcgaac agcgattgtt cctgcgcgcg atcatgggtc gcgttgcggc
     1081 agagggcgag aatgacggtt tgggtaccag tcgtgacggc ctgcaatacc acgtcgaccc
     1141 aatagatccc gccttggaga tcgcgtccct gccagcgaag tttctgacgc ccttctgatg
     1201 ccgcttttcg cacccaggtc acggcatcag ccacgctgta tccgggctcg ctggtgagaa
     1261 agtgctcgag aggcaggttt cgtaactcgt ccatggtaca gcgagtcatc gccagggcac
     1321 aggcgttggc atccagaaga cgggccgttt ctggctcaac gacgaacacg cccatgtcga
     1381 ccaggtcgaa aagggctccg taatccagag atacctgatg cacgacgatt actctcgctg
     1441 aggatagtgg ggtgcggaaa agtgcaacga tgctactctg ttcgcgtgga tttgacgacc
     1501 acacaggtca gatcgtcggt aaagctgtcg gaactgacga aggtgcgata ggtgccgatg
     1561 atcgaggtca gaatcgcatg cgccggctct gagcgtagac catgaatcag ttcctgcagc
     1621 cgctcgatgc cgaagaacgc tccgttggca tcccgtccgt ccatcaggcc gtcggagtag
     1681 aagacaaaga cgtcatcagg ttcgaacgac acggtggttt gctgatagtc gcggctttcc
     1741 gggaacccga gcggaaagcc ttctcccttg agttgtacgg ctccttgcgg cgtggaccag
     1801 agcgcaggtg ggtgtccggc gtcgacgaac gacatggttc tcgtctgtgt gttgaagcgg
     1861 gcatagcaca aggtgacaaa gcgtccgagc gacgccaggt gattcccggc ttcactctgg
     1921 acccgagtca tgacggcttc aggctccggc agggccgctt ctcccagatt gcgcagcaac
     1981 tgagccagca ctcgcgaaaa ccggtttttc gttcccgcgc cgacgagggc cgcggggatt
     2041 cccttgccca tgacatcgcc gagaatcaga tcaaaacagg tgttatcgtg aacgaaaaaa
     2101 tcgagaaagt cgccgtcgac cccggccgag gcctgggtaa aacaggcgat ctgggcaccg
     2161 tgaatctgcg tcggcggagt ttcgaacagc agagtctgct gaatctcccc cgcgatgcgt
     2221 atctcctggc gtcgcgcctc tttttccgcc tgtatcaacc ggtactgttc gactccggct
     2281 tcgagtgccc gcagcagatc gggctgcgaa cagggcttcg tcagaaagcg aaagacgctg
     2341 ccttcattca ccgccttgat ggcgatctgg aaatcgccct caccggtcag catcatccgc
     2401 acggtttcag gggctttctc gcgtgcgagc gacaaaaaac gaatgccatc catgcggggc
     2461 atgttgaagt cggaaataat cacggcgaac ggtccctgct cgtcgatcag cttcagacca
     2521 gcttcaccgc tgagagccgt aaacaggtga aactgccggc cgagaattct tccgtacgag
     2581 tcgagaatat tcggttcatc gtctaccagc aggattcgcg gtttcatcgt cgtttgcttc
     2641 tttcccaggc gtggtcagcc agaatttccc agctgcggag atggtcggtg agtccgagaa
     2701 agctgagata ctcggaatcg ggttttgtcg gtctgccggc acgggtctcg agcagggcgt
     2761 tggccacgtg aatggcggtg aggggcgaaa acccttcggg tggaatctgc agcggccggt
     2821 gatggcaagc cacggtgagg acggtgacgt cgggcaggcc ccagatgcca agcagatagc
     2881 cgccgatttc ggcgtgagac gtttcaaa
//
LOCUS       DAORHD010000569         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40050_length_2898_cov_4.115371, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000569 DAORHD010000000
VERSION     DAORHD010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40050_length_2898_cov_4.115371"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKO06_12250"
     CDS             <1..454
                     /locus_tag="PKO06_12250"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HNV70463.1"
                     /translation="IDHIDSWVALFSESLPGPVTRLVKVAEVKRRDQNIAGISLPFLD
                     SVTYEILPYSRKSTPLWVDCGVEIVKELMELREKAKVMQDAEKVLQHELRKVTQRLNL
                     FEKVKIPQSQEAIRVIRIALADEQTAGVGRAKIAKAKTLAEPTTEVSS"
     gene            451..2277
                     /locus_tag="PKO06_12255"
     CDS             451..2277
                     /locus_tag="PKO06_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70464.1"
                     /translation="MITPMKKVFVAGREADRPDVLSVLQEAGVMHVVPVDAGAMKPTI
                     EVNQDIERAERAIGGLAEVQTEGSPLEYPGTPSRLVDEYYELSERVQWNAAMQAETQK
                     KIQDTALWGALDIESLDVLRRNGLTIKFCLGTSDAVRAIEADAMQVVREESGMAYTIV
                     ASRSPIGLPPGVNEIPAPMREARDLEQDLEKLKAEKRDLLESLQRIRLRLPDIQTYLT
                     DLRERKRFQEVEMGLLAEGPIFVCTGWVPVDAISDLEGRLLKSGKRVALQAADPDDED
                     APPTKFLNAAWCKPIEALYGAFGVFPGYREPDISQYFLPFLALFTAMLIGDGGYAVIG
                     LAVLGSIYRPAVRNGVPAQFLQLFMILFGATFVFGALTNTWFGTQVSFLKGLMAIDPG
                     TPAGENILKKLCFAIGGLHLTIAHVWKTIRKPQVVEAASLAEVGWIIFIWSIFDLVNF
                     LVLGETLPGRTLPMIYVALGLVLFFSSPSWNPLAAAWSGAGVIALNAAAFLSDIISYI
                     RLWAVGYAGGILASSFNSMIGGIWSYESFQPAPMVVAALLFIAAHLLNFGLGLVAIFA
                     HGVRLNLLEFSNHLGIEWVGKPYDPFRRLSGGEPAAGSEKNL"
     gene            2293..2754
                     /locus_tag="PKO06_12260"
     CDS             2293..2754
                     /locus_tag="PKO06_12260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNV70465.1"
                     /translation="MFLDIVGKLSGGLVLGLGGIGSCLGILAAAQAAAGAWAAEGKAG
                     KNPSFQYVIMVAAPISQTLYAMIVMMKIMGFKTITPDVGMLMLGTAIGVGIIELASAW
                     GQGLIGAAGVRCLNENGGKGFGFIIVALGIIETVGIFGMVFGLQLLGSVAQ"
BASE COUNT          560 a          759 c          903 g          676 t
ORIGIN      
        1 gatcgaccac atcgattcct gggtggcgct gttcagcgag tcgcttccgg gtccggtcac
       61 caggctggtg aaagtggctg aagtgaaacg acgtgaccag aacattgcgg ggatctccct
      121 cccgtttctc gattccgtga cctacgaaat ccttccctat agccgaaagt cgacacccct
      181 ctgggtcgat tgcggggtgg aaatcgtgaa ggaactcatg gagttgcgcg agaaagcgaa
      241 ggtcatgcag gatgccgaga aagtgctgca gcatgaactg cgcaaggtga cgcaacgcct
      301 gaatctcttc gagaaggtga agattcctca gtctcaagag gcgattcggg tcattcgtat
      361 cgctctcgct gatgaacaga cggcgggtgt tggccgtgcc aagatcgcca aggccaagac
      421 gcttgccgaa ccgacgacgg aggtgagttc atgatcactc cgatgaagaa agtctttgtc
      481 gccggtcgcg aagctgacag gccggacgtg ttgtcggttc tgcaggaagc tggcgttatg
      541 catgttgtcc cagttgatgc cggcgcaatg aagccgacga tcgaggtcaa ccaggacatc
      601 gaacgagcgg agcgagccat tggcggactg gccgaagtcc agacggaagg gtcgccgctc
      661 gagtatccgg ggaccccctc acgtttggtt gatgagtatt acgaattgtc cgaacgagta
      721 cagtggaatg ctgcaatgca ggccgagacg cagaagaaga ttcaggatac cgccctgtgg
      781 ggggctctgg acatcgagtc gctcgatgtt ctgcgccgga acggcctgac gatcaaattt
      841 tgtctgggaa cgagcgacgc ggttcgggca atcgaggccg atgcgatgca ggtcgtgcgc
      901 gaagaaagcg gaatggcgta tacgatcgtc gcgtcccgga gtcccatcgg gctgcccccc
      961 ggggtgaacg agattcctgc gcccatgcgc gaagccagag acctggagca ggatctcgaa
     1021 aagctgaagg ccgagaaacg cgatctgttg gagagcctgc agcgcattcg tctgcgcctg
     1081 ccggacattc agacctatct caccgatctg cgcgagcgca aacgttttca agaagtcgaa
     1141 atgggtcttc tggctgaggg cccgatcttt gtttgtaccg gttgggttcc cgtagatgcg
     1201 atctctgatc tcgaaggcag gctgttgaaa tccggaaaac gcgtggcgct tcaggccgcg
     1261 gatcctgacg atgaagacgc gccgccgacc aagttcctga atgcggcctg gtgcaagccg
     1321 atcgaggcgc tgtacggcgc gttcggggtt ttcccgggct atcgggagcc ggacatcagc
     1381 cagtatttct tgccatttct ggctctgttc accgccatgc tgatcggtga tggcgggtat
     1441 gccgtgatcg ggttggccgt tctgggatcg atctatcggc ctgctgtccg caacggagtt
     1501 cccgcccagt ttctgcaatt gttcatgatt ctgttcgggg cgacgtttgt ctttggcgcc
     1561 ctgaccaaca cctggtttgg cacccaggtc agctttctca aagggttgat ggcgattgat
     1621 cccggaacgc cggccggaga gaacattctc aagaagctgt gctttgcgat tggcggcctg
     1681 catctgacga ttgcccatgt ctggaagacc attcgcaaac cgcaggtcgt tgaggcggct
     1741 tctctggcag aggtgggctg gatcatcttc atctggtcga tcttcgatct ggtgaacttt
     1801 ctcgtgctgg gtgaaacgct tccggggcgc acgctgccca tgatttacgt tgcgctcggg
     1861 ctggtactct tcttttcatc accatcgtgg aatccgttgg cagccgcctg gagcggtgcc
     1921 ggtgtcattg ccttgaatgc tgcagcgttt ctctcagaca tcatcagcta tatccgtctc
     1981 tgggccgtcg gctatgccgg tggaattctc gcgtcgagct tcaattccat gattggcggg
     2041 atctggtcct atgaatcgtt tcaaccggcc cccatggtcg ttgcagcgct tctcttcatt
     2101 gcggcgcatc tgctcaactt cgggcttggc ctggtcgcga tcttcgcgca tggagtgcgg
     2161 ttgaatctgc tggagttctc gaatcatctc ggcattgagt gggtcggaaa gccgtatgat
     2221 ccgtttcgtc gtttatccgg tggcgaacct gctgccggct cggaaaaaaa cctttgatcg
     2281 aaggagaatc atatgtttct tgacatcgta gggaagctgt ctggcgggtt ggttctgggt
     2341 cttggcggta tcggcagttg tctgggaatt ctggcagcgg ctcaggctgc ggccggggcc
     2401 tgggcggccg aaggcaaggc gggcaagaac ccgtcttttc aatacgtcat tatggttgcc
     2461 gcgcccatca gccagacgct gtatgccatg atcgtcatga tgaaaatcat gggtttcaag
     2521 acgatcacgc ccgatgtcgg catgctcatg ctcggtacgg ccatcggtgt cggaatcatt
     2581 gagttggctt ccgcgtgggg tcagggtctg atcggcgctg ccggtgtccg ctgcttgaac
     2641 gagaacggcg gcaagggctt cggcttcatc atcgtggctc tcgggatcat cgagaccgtc
     2701 ggcatcttcg gcatggtctt cgggctgcag ctgctgggtt cggttgccca ataggctctt
     2761 tttcgcttct acggctgaaa agcgccctga tcgcttcgtg cgatcagggc gcttttcatg
     2821 tgcgcgaaaa aagtgcttga cgaaatagac gaattaaggt attctaatgc cagaataacg
     2881 aaaggcgcga aacatgac
//
LOCUS       DAORHD010000570         2897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40077_length_2897_cov_3.426460, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000570 DAORHD010000000
VERSION     DAORHD010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40077_length_2897_cov_3.426460"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PKO06_12265"
     CDS             <1..593
                     /locus_tag="PKO06_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70466.1"
                     /translation="VQTIQPSDAAIGGVAPALRPHSHGEEEEEGTAGEADDGADDPNV
                     EAGDSRPLVPLAVTVNRAVNLLEEALFGSLVVRSGAATGWKMSCVPVDIDGQSLSVMV
                     VATAKFNNQQRAVRVLIADPFEAAFASRAAVSAGMTDQVGLHFHVGEWFANLGLEALV
                     QAGGGEIVIDEEAHEMHEKLLNNVKASTAYEGETHE"
     gene            622..1548
                     /locus_tag="PKO06_12270"
     CDS             622..1548
                     /locus_tag="PKO06_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNV70467.1"
                     /translation="MTRTVVLFLAITCGLLWGGEAQPIYGAIEVVTTTTDLAAIVKAV
                     GGERVRVSAIGKGHEDPHFVEPKPSYMMQLSRARLLFCVGLELEIWLRPLIEGARNLT
                     IQPGSAGYVDCSTVIDIKEIPKTRVDPSMGDVHPLGNPHYWLDPENGCRIADLAAQKL
                     SEVDPPGAAIFATNAKAFRVDIESRLPNWRAALSGFKNRGVACFHSSWIYFTEAFGLQ
                     IVGYVEPRPGVPPTGRELASLVAQMKARGVKTVLREQYHSDRFANMVAEKAGGNVVVL
                     PASVGAEARLATYADLFDVLIEKVSVALAHQQ"
     assembly_gap    1666..1675
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1676..2307
                     /locus_tag="PKO06_12275"
     CDS             <1676..2307
                     /locus_tag="PKO06_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV70468.1"
                     /translation="GKTTILKAILGLLAPAAGSIQHPGPGIQRVGYVPQRGAIDEVFP
                     FTLAEIVEMGFYGVNRPWARLTTEQNTRVGQVLKAVGLDSMTNRLFRELSGGQKQRAL
                     LARALVAEPQVLVLDEPTHGLDIAQARTLLALLDDLRLHQQLSLIVVSHNLVELLDHC
                     QRLLLLHHGQLAFCGVPGDLTDEVLERIYGMPVRLRGAGTPLLYQDDVV"
     gene            2304..>2897
                     /locus_tag="PKO06_12280"
     CDS             2304..>2897
                     /locus_tag="PKO06_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNV70469.1"
                     /translation="MNHTATFTELVQFFHDAILVAVLTGAILGPVGLFLHLRRSLFLG
                     AALPQVAGFAFVVATVVGIPTWLSAMCTILIFSLFAAWKPVQATDGLTLETLIALAYT
                     VSMAGIILLLALTQAEAHAAELLLKGSVLAATCQDTRLLAWAGIPLVIVLYLFRRVLY
                     LVSLDPETARTLGISVWKYETLLFLALGLAITLTLGEA"
BASE COUNT          526 a          815 c          924 g          622 t
ORIGIN      
        1 gcgtgcagac gattcagcct tctgacgccg cgatcggagg tgtcgcgcca gcgttgcgcc
       61 cacacagcca cggggaggag gaagaggaag gaacggccgg ggaggcggat gacggcgcgg
      121 atgacccgaa cgtcgaagca ggagattcgc gccccctggt gcctctggcg gtgacggtca
      181 accgagcagt gaatctgctc gaagaagcgc tgttcggttc cctcgtcgtg cgctctgggg
      241 cggcgacggg gtggaagatg tcatgcgtgc cagtcgacat tgatggacaa tcgctctcag
      301 tcatggtcgt ggcgacggct aagttcaaca accagcaacg tgctgttcgc gtgttgatcg
      361 ctgatccgtt cgaagcggct tttgcttcac gggcggcggt ttcagcaggg atgaccgatc
      421 aggtggggtt gcactttcat gtcggcgagt ggtttgccaa tctcgggctc gaagctctgg
      481 tacaagcggg cggtggtgag atcgtcatcg acgaagaagc tcacgagatg catgagaaac
      541 tgttgaacaa tgtgaaagcc tcaacggcgt atgaaggaga aactcatgaa taggaacaca
      601 caatcgagat ggtgccaatc tgtgacccgg acggtggttc tgttcctggc gatcacctgc
      661 ggcctgttgt ggggtggcga ggcgcaaccg atctacggag cgatcgaggt ggtgacgacc
      721 accaccgatc tcgctgccat cgtcaaggca gtcgggggag agcgtgtccg agtctctgcc
      781 atcggaaaag gtcatgaaga cccgcatttc gtcgagccca aacccagcta catgatgcag
      841 ttgtcccggg cgcgcctgct tttctgcgtc ggtctcgaac ttgaaatctg gctcaggccg
      901 ctgatcgaag gtgcgcgaaa cctgacgatt cagccgggga gcgccggcta tgtcgattgt
      961 tctaccgtga ttgacatcaa agagattccg aagacgcggg ttgacccgtc gatgggcgac
     1021 gtgcatccgc ttggaaaccc tcactactgg cttgacccag agaacgggtg tcgcatcgct
     1081 gatctggcgg cgcagaagct gtccgaggtc gatccgcccg gagccgcaat cttcgcgacg
     1141 aatgccaagg cttttcgggt cgacatcgag agccgcctgc cgaattggcg cgcggcgctg
     1201 tcggggttca agaatcgcgg ggtggcctgc tttcattctt cctggatcta cttcacagag
     1261 gcgtttggcc tgcagatcgt cggttatgtc gaaccccggc ccggggtgcc gccgactggg
     1321 cgcgaactgg cttcgctggt cgcacagatg aaggcgcgcg gggtcaaaac cgtgttgcgg
     1381 gaacagtatc actcggatcg atttgccaac atggttgccg aaaaagctgg tggcaacgtc
     1441 gtggttctgc ccgcctcggt gggcgcggaa gcgcgattgg cgacctatgc agatttgttc
     1501 gacgttctga tcgagaaagt gtctgtagct ttggctcatc aacaatgaat tgggtagaat
     1561 tccaacaagt acgtttaaga tacggaaggc atgtcgtact cgacgacctg acctttgcgt
     1621 tgccggccgg ccaggcggtt ggtctggccg gcccgaatgg atcggnnnnn nnnnncgggc
     1681 aagactacga ttctcaaggc aattctgggt ctgctggctc cggccgcagg atcaattcag
     1741 catccgggcc ccggaattca acgggtcggg tatgtcccgc agcgtggggc aatcgacgag
     1801 gtgtttccct tcacgctcgc tgagattgtt gaaatggggt tttacggtgt caaccgaccg
     1861 tgggcacgcc tcaccaccga acagaatacc cgggtggggc aggttctcaa ggcggtcggg
     1921 ctcgactcga tgaccaatcg actgttccgt gagctttcgg gggggcagaa gcagcgggcg
     1981 ttactggcac gcgccctggt ggctgaacct caggttctgg tgctggatga accgacccat
     2041 ggcctcgaca ttgcacaggc gcgcacgctc cttgccttgc tcgacgacct gcggttgcat
     2101 cagcagcttt ccctgatcgt ggtgtcacac aacctcgtcg aactgctcga ccattgccag
     2161 cggctgctgt tgctgcacca tgggcagctg gccttctgcg gcgttcccgg agacctgacc
     2221 gacgaagtgc tcgagcgcat ctacggcatg ccggtccgtc tgcgtggcgc tggaacccca
     2281 ttactgtatc aggatgacgt cgtatgaacc acactgccac attcaccgaa ctcgtacagt
     2341 tctttcacga tgcgattctg gtggcggtgc tgaccggagc catcctcgga ccggtcggcc
     2401 tgtttctgca tctgcggcgc agtctctttc tcggagccgc gttgccgcag gtcgctggct
     2461 ttgcgtttgt cgtcgcgacc gtcgtgggca ttccgacctg gctgtcggcc atgtgtacca
     2521 ttctgatctt ctcgctcttc gccgcctgga agccggtgca ggcgacggac gggctgacgc
     2581 tggaaacgct gatcgcgctc gcgtataccg tgtcgatggc cggcatcatc ctgctgctgg
     2641 cactgacgca ggcagaagcc catgccgcgg aactgcttct gaaaggcagt gttctggccg
     2701 cgacctgtca ggacacgcgt ctgttagcct gggctggaat cccgctggtc atcgtgctct
     2761 acctctttcg tcgcgtgctg tatctcgtct cgcttgaccc cgagaccgcg agaacgctgg
     2821 gcatttcagt ctggaaatac gagactctgc tgttccttgc tttgggcctg gcgattaccc
     2881 tgacgcttgg tgaggcg
//
LOCUS       DAORHD010000571         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40163_length_2894_cov_2.434660, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000571 DAORHD010000000
VERSION     DAORHD010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40163_length_2894_cov_2.434660"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1582)
                     /locus_tag="PKO06_12285"
     CDS             complement(<1..1582)
                     /locus_tag="PKO06_12285"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNV70470.1"
                     /translation="MLALLFSLFLLVVVISGIAGIVQFINRLTGSSSATPELETVRDE
                     LRSLNTRIDLQTRRQKSLEQKIERLTTEVQALRQRTAPPSVPSATALEPVLNLTVPEV
                     SKTPESQEPRQIQPDSAAIPVPPVIAEPIVVPPPTPEIEFTPPKHRDLEAAVTTPPVD
                     LVSPPPEPAVAQSSPLPAIAQSLTPELPPIPTNDPSPQASKEDPATYQPGQTPAGSDE
                     EAKGWFSGFDWENFLGVKMFAWLGGLALFLGVSFFIKYSVENNLISPEARFYIGLLIG
                     FGALVGGLELRRRTGDLYEFTVHALCASGIAILYSDIIAARVLLDYTSITITFLLMVS
                     VTTTAFLLSVQLNARFVAILGLLGGFLTPPILSTGQDNPVGLFLYIALLNGGLAAVAI
                     RKHWGFLVALGACGTALMQIGWHHRFFTPDKLAIAWVIFGGFSLFYACVREIARRRFQ
                     YDDLWMSLTSCWFPIISMWFPASLLKVRQVLGHEEAVFSLQFLNAATIALQVPHDMQA
                     RPAMGVSLSISLLFMLVWG"
     gene            complement(1659..>2894)
                     /locus_tag="PKO06_12290"
     CDS             complement(1659..>2894)
                     /locus_tag="PKO06_12290"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNV70471.1"
                     /translation="TEKRPMAQRRSLMEDPDPVVRKAAFDGGNKVWDQYADVCASAIN
                     HMAGTRLTLNKHRGINHFLDVALFQARISRPTLDALFTALEKTIDIPRRMARAKAKAL
                     GLKALGWQDQEAPLPIKNEKRFEWEPGKIRLKEAFNAVFPELASFLQMAIDKNWIESE
                     VKSGKRPGAYCTSSPFIRESRVYMTFGGSLGDLFTLAHEIGHAYHTYVMRDLRPFAQS
                     YPMPLAESASTFAEMIFSDGVMADPEIPPALKANLLNMKMGDAIAFLLNIPTRFTFEK
                     NFHEERMKGEVGAERLKELMVQAQRQYYADTLTDGQEDPFFWASKLHFYITEVTFYNF
                     PYTFGFLLSRGLFARFKKEGADFLPKYKEFLRLSGSYSCEEVAKKALGIDLKKPDFWI
                     STIRSMEDDLKLFEKFVLK"
BASE COUNT          662 a          747 c          900 g          585 t
ORIGIN      
        1 ggccccagac aagcataaac agcagactga tcgagagact gacccccatc gcgggcctcg
       61 cctgcatatc gtgtggaacc tgcagcgcaa tcgtcgcagc attcaaaaac tgaagcgaga
      121 agacggcttc ctcgtgtccg agaacttggc gaactttcag caatgatgcc ggaaaccaca
      181 tggatatgat cggaaaccag caggaggtca gcgacatcca aagatcgtca tactgaaagc
      241 gacgccgggc gatttcgcga acacaggcat agaagaggga gaaacccccg aatatcaccc
      301 aggcgatggc gagtttgtca ggcgtgaaaa accgatgatg ccagccgatc tgcatcagag
      361 ctgttccaca ggctccgagt gcgaccagaa atccccagtg ctttcggatt gccactgcag
      421 ccagaccgcc gttgagcaga gcgatgtaga gaaacaggcc gacgggattg tcttggccgg
      481 tggagagaat cggaggagtc aggaagccgc cgaggagccc cagaattgcc acaaagcgcg
      541 cgttgagttg tacggaaagg agaaacgcgg tcgtggtcac ggagaccata agaagaaacg
      601 tgatggtgat gctggtgtaa tcgagaagaa cccgggcggc gatgatatcg gaatagagta
      661 tcgcgatacc ggaagcgcac agggcgtgaa cggtgaactc gtacagatcg ccggtgcgtc
      721 gacggagttc gagaccgccg accagtgcac cgaacccgat gagcaacccg atgtagaacc
      781 gcgcttcggg agaaatcagg ttgttctcga cggaatactt gatgaagaag ctcacgccga
      841 gaaacagagc cagaccgcca agccacgcaa acatcttgac gccgagaaag ttttcccagt
      901 caaagccgga gaaccacccc ttcgcttctt catcagagcc tgcaggtgtt tgtccaggtt
      961 ggtatgttgc aggatcttct ttagaagcct gtggggaagg atcgttcgtg ggaatcgggg
     1021 gcagttctgg ggtgagactc tgtgcgatgg cgggcaacgg actggattga gcaaccgcag
     1081 gctccggggg tggggacaca agatcgaccg gtggagtagt aaccgcggct tccaggtctc
     1141 tgtgtttcgg gggagtgaac tcgatctccg gtgtgggagg gggaaccacg attggttcag
     1201 caatgaccgg cggaacagga attgcggcac tgtcgggctg gatttgacgt ggttcttgag
     1261 attcaggtgt cttggagacc tctggaaccg tgagattcaa caccggctcc agcgctgtcg
     1321 ccgatgggac gcttggaggg gccgtccgct gtcgcagcgc ctgcacctca gtggtcagtc
     1381 gctcgatctt ctgttcgaga cttttctgcc ttcgggtctg cagatcaatg cgggtattga
     1441 gactgcgaag ttcatcacgc acggtttcga gttcgggggt cgcgctcgat gagccggtaa
     1501 ggcggttgat gaactggacg attccggcaa tgcctgagat gaccacaacc agcaggaaca
     1561 aggaaaagag taatgccagc atggtcagct cctgtctcgg gttgccaggg gcggcgagcg
     1621 atgaaccacc tccccagggc agggaaggtg gttgatcgtt acttcagaac gaacttctcg
     1681 aacagcttga gatcgtcttc catactccgg atcgtactga tccagaaatc cggctttttc
     1741 agatcgatgc cgagcgcttt cttggccacc tcttcgcatg aataggagcc actgagacgc
     1801 agaaactcct tgtacttcgg caggaagtca gctccctcct tcttgaagcg ggcgaaaaga
     1861 ccgcgactga gcaggaaccc gaaggtatag ggaaagttgt agaacgtgac ttccgtaatg
     1921 tagaagtgca gtttcgaggc ccagaaaaag gggtcttcct gaccgtcggt gagcgtgtca
     1981 gcgtaatact gccgctgggc ctgaaccatc aactctttca agcgttcagc ccccacttcg
     2041 cccttcatgc gttcctcgtg aaagttcttt tcgaaagtga atcgggtggg aatgttcaag
     2101 agaaacgcga tggcatcgcc catcttcatg ttgagcaaat tggctttcaa ggcgggcggg
     2161 atctcgggat cggccatgac accgtcagag aagatcattt cggcgaaggt cgaagctgac
     2221 tcggcgagcg gcatcggata ggactgggcg aacggtcgca gatcgcgcat cacataggtg
     2281 tgataggcat gaccgatctc atgggcgagg gtaaacagat cgcccagact gccgccgaac
     2341 gtcatgtaga cgcgggattc gcgaatgaac ggcgatgagg tgcagtaggc tccgggccgc
     2401 ttgccggatt tgacctctga ttcgatccag ttcttgtcga tcgccatctg cagaaagctg
     2461 gcgagttcag gaaagacggc attgaacgct tctttcagcc ggatcttgcc cggttcccac
     2521 tcgaagcgtt tttcgttctt gatcggcagc ggtgcttcct ggtcttgcca gcccagcgct
     2581 ttcaagccga gggcttttgc cttggcgcgc gccatgcggc gcggaatgtc gatggttttc
     2641 tcaagcgcgg taaacagggc atcgagagtc ggtcgcgaga tgcgggcctg gaacagggcc
     2701 acatcgagaa agtggttgat cccgcgatgc ttgttcagag tcaggcgagt tccggccatg
     2761 tgattgatgg cgctggcgca gacgtcggcg tactggtccc agactttgtt gccgccgtcg
     2821 aaagccgcct tgcgaaccac cggatcagga tcttccatca ggctgcggcg ctgagccatg
     2881 ggacgcttct cggt
//
LOCUS       DAORHD010000572         2888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40280_length_2888_cov_3.909636, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000572 DAORHD010000000
VERSION     DAORHD010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40280_length_2888_cov_3.909636"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKO06_12295"
     CDS             complement(<1..183)
                     /locus_tag="PKO06_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70472.1"
                     /translation="MKRYALIRLTLPVAVLGCLLTLPTETQARNDRRRPVSPPTIEVS
                     PTPGFESAARQHQIRSA"
     gene            complement(184..1131)
                     /locus_tag="PKO06_12300"
     CDS             complement(184..1131)
                     /locus_tag="PKO06_12300"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNV70473.1"
                     /translation="MQKASKVKIFSGGSNNELVEEICKYLDVPVGKIYSNTFADGEFY
                     CRIEESVRGCDTFIIQPTCPSVNQNLMTLLIMIDALKRASAESVTAVVPYFGYSRQEK
                     KTLGREPITAKLVANLVATAGADRIVTVDMHDPALQGFFDIPVDHLSAIPQLARHLLR
                     SDLSNSVVIAPDTGGVHRARDFARRLKLPMAIIDKRRPEQNKAVVMNVIGEVSGKDAI
                     IIDDIIDTAGTLVQVAEALKSKGVLKVTACCTHALLSPPATDRIASSCLTRLVTSNSI
                     PLTEKKKQVPMLETISLAQLIGETIFRIFSKKSVSELFF"
     gene            complement(1135..2169)
                     /locus_tag="PKO06_12305"
     CDS             complement(1135..2169)
                     /locus_tag="PKO06_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNV70474.1"
                     /translation="MHPFDSLILAAGKGVRMKSNLPKVLHPVLGKPMVQYVIETVRRV
                     GSAHSHVIIGFGKDQMRQALSTQGVGFIEQNEQLGTGHAVQCFAREHPTPPEHLLVVC
                     GDTPLLSEETLRTMIEQHFSLRPAATMMTLDMQEPGGYGRILRNQQGNVQEIREAKDC
                     SEVEKRIREVNLAIYLFEGKALYDRLFSLKNENRQKEYYLTDVIGMMTRDGLPVIAVK
                     ERDEASTLGINSRLDLNRVAGILRQRIIEKHLSEGVSILDPATTWIEPDVTIGSETVI
                     YPNTILTGKTVIDSGCRIGPQVRVTGAHLNTGVTAEFAVIEHVTVAPHTRVKPFSHLG
                     TEQAHSEARE"
     gene            complement(2358..2429)
                     /locus_tag="PKO06_12310"
     tRNA            complement(2358..2429)
                     /locus_tag="PKO06_12310"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2395..2397),aa:Gln,seq:ttg)
     gene            complement(2489..2794)
                     /locus_tag="PKO06_12315"
     CDS             complement(2489..2794)
                     /locus_tag="PKO06_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HNV70475.1"
                     /translation="MVITEIRIYLSSVADSTIRAFASVTFDGSFVVHGLKIIESRGRL
                     FVSMPARKMPTGEFREIAHAITTELHEAIQSQVLRAYDDERHRPEHTTSASPARSDP"
BASE COUNT          598 a          777 c          886 g          627 t
ORIGIN      
        1 cgccgaacgg atctgatgct ggcgtgcggc tgactcaaag ccgggggtcg gagagacctc
       61 gatcgtgggc ggactgaccg gtcgtcgtcg atcgttgcgg gcctgagttt ctgtcggaag
      121 agtcagcaga cagcccagaa ccgcgacggg aagagtgagt cggattaatg cataccgctt
      181 catctagaaa aacaactcgc tgacggactt ctttgagaag atacggaaaa tcgtttctcc
      241 gatcaactgg gccagggaaa tcgtttcgag catgggcacc tgcttcttct tctcggtcag
      301 cggaattgaa ttggaagtga ccagccgggt gagacacgaa ctggcaatgc ggtcggttgc
      361 cggtggagac aacagcgcat gcgtgcagca ggcggtgact ttcagcacac cctttgattt
      421 caacgcttcg gcgacttgta caagtgtgcc ggcggtatcg atgatgtcgt cgatgatgat
      481 tgcatctttc cctgagactt caccgatgac gttcatgacg acagccttgt tctgctcggg
      541 tcggcgcttg tcgataatcg ccatcggcag cttcagacgt cgggcgaaat cacgggcccg
      601 atgcacgccg ccggtatccg gggcgatgac gaccgagttg ctcaggtcgc tgcgcagcag
      661 atgtcgtgcc agctgcggaa tcgccgacag gtgatcgacg gggatatcaa agaacccctg
      721 caaggccgga tcatgcatgt cgacggtcac aatgcgatcg gcacccgctg ttgccaccag
      781 gttggcgacc agtttggccg tgatcggctc gcggcccagc gttttctttt cctgacgtga
      841 gtagccgaaa tacggaacca cggcagtgac gctctcggca gaagcgcgct tgagcgcatc
      901 gatcatgatc agcaacgtca tcaggttctg attcaccgag ggacaggtcg gctggatgat
      961 gaaggtatcg cagccgcgga ccgattcttc gattcggcag tagaactcgc cgtcggcgaa
     1021 cgtgttggag tagatcttcc cgaccggaac atcgaggtat ttgcagatct cctcgaccag
     1081 ttcgttattg ctgccgcctg agaagatctt cactttgctg gctttctgca tacgctactc
     1141 cctggcctcg gaatgggcct gttcggttcc aagatgagag aacggcttga cgcgggtatg
     1201 cggggccacc gtcacatgct cgatgacggc aaactccgcg gtcactccgg tgttcagatg
     1261 cgcccccgtg acgcgaacct gcggcccgat gcggcaaccg ctgtcgatga cggttttgcc
     1321 cgtcaggatc gtgttcgggt agatcacggt ttcagagccg atggtgacgt ctggttcgat
     1381 ccaggtggtg gccggatcga ggatggaaac cccttcgctc aggtgcttct cgatgatgcg
     1441 ctgccgaaga atgccggcga cgcggttcag atcgaggcga ctgttgatgc cgagtgtcga
     1501 ggcttcgtca cgctctttga ctgcgatgac cggcagaccg tcacgcgtca tcatgccgat
     1561 gacatcggtc aggtagtact ctttctggcg attctcgttc ttcagagaaa acaggcgatc
     1621 gtacaaggcc ttgccttcga aaaggtagat ggccagattc acttcgcgaa tacgtttttc
     1681 tacttcggaa cagtcttttg cctcacggat ctcctggacg ttcccctgct gattacgaag
     1741 gatgcgcccg tagccgccgg gttcctgcat gtcgagggtc atcatggtgg cggccgggcg
     1801 cagcgagaaa tgctgctcga tcatggtgcg cagggtttct tcggacaaca ggggggtgtc
     1861 gccacagacg acaagcaggt gttcgggtgg ggtcggatgt tcacgggcga agcactgaac
     1921 cgcgtggccg gtaccgagtt gctcgttctg ctcgatgaac ccgacgccct gcgtgctcaa
     1981 agcctgtcgc atctggtctt tcccgaaccc gatgatgaca tgcgagtggg cagacccgac
     2041 acggcgcacg gtctcgatga catactgcac catgggcttg ccgagaaccg gatgcagcac
     2101 tttgggaaga ttcgatttca tccggacgcc tttgccggcg gcgagaatga gagagtcaaa
     2161 cggatgcatg atgaggcctc atgatgctga tggaatgcgc ttcaagacat accggaccga
     2221 ggactgtcgt gtggcgagcc aggtcacagt cgtaagacat gacgtcaact ctctccctct
     2281 catgttgtcc ggttcagatg ttttcgatct gcttacttgt actccggcac gcatgccgac
     2341 gagcggaaaa atctgagcag gggtgcaagg attcgaacct tggaatgctt gctccaaagg
     2401 caagtgcctt accacttggc gacacccctc tgtgatgatt gatcaccggc ctgaccatat
     2461 cacaaaaccc gcttcggcgc aagtttcctc aggggtcaga gcgagccggc gatgcggatg
     2521 tggtgtgttc ggggcgatgc ctctcgtcgt catatgcccg caagacctgg ctctggatgg
     2581 cctcatgcag ttcagtggtg atggcatgcg cgatctcgcg aaactcgcca gtcggcattt
     2641 ttcgggccgg catcgagacg aacagccgac cgcggctctc gatgattttc aagccatgaa
     2701 caacaaatga gccgtcaaac gtgacggaag caaaggcgcg aatagtcgag tcggcgacgg
     2761 aagagaggta gatccggatc tcggtgatga ccatgaaatc acggcagggg aggcatgcaa
     2821 tgcaccgtga cggtcagaac ttgaggatga attcggtgaa gacttttttg tgcccggaac
     2881 cctgagga
//
LOCUS       DAORHD010000573         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40419_length_2882_cov_2.736470, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000573 DAORHD010000000
VERSION     DAORHD010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40419_length_2882_cov_2.736470"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PKO06_12320"
     CDS             complement(<1..484)
                     /locus_tag="PKO06_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70476.1"
                     /translation="MMIHRLPATTRGFVPRFCLCISLLVALVALPQDAAALMFRAKPT
                     LTLPDFTGQSEHTGRNPIIAAARENFSEYTQSLQRDQALFFEAADRLELMTRILTPRI
                     PLFEKVAKENALATPGEIWRVMNAWAHYLHHLGRLEMLARTYHFSMTDLWKDDEKTAF
                     E"
     gene            651..1241
                     /locus_tag="PKO06_12325"
     CDS             651..1241
                     /locus_tag="PKO06_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNV70477.1"
                     /translation="MNDAKPKLPYTVAFMIPPEAINLLVFWRKKVAPFCEKIDPLEVA
                     HMTVKYLGFPSETFREESVVSLIPRLAKIVAPHLPVRVSVRGLDLFDCHCEPVVYLKV
                     VSGGALAEIHDAIRVGLGKDIETFPHADGENYRPHITISKQIKHRQIDALRQLIHRSK
                     KSAKRTFKLTQLVLLTPWDIYPILPLVGRKHPPTNW"
     gene            1367..1888
                     /locus_tag="PKO06_12330"
     CDS             1367..1888
                     /locus_tag="PKO06_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70478.1"
                     /translation="MLVTRNSCTIWLPVLILVGLLTVVPGQAQFQTAPAPAPTMDQAT
                     GGAPSSCCGQHCSLSCRHGFYTTDGMNVYWFPHGYPRSIHRVQSNTNTGPDLRFATRY
                     RWSHRHYHACACHPVYQMQFKGPYWTHTTYRPARGLLHRSTWRCTWIPYYVAAIPNAS
                     VLGTVPHTSNPVP"
     gene            complement(2031..2420)
                     /locus_tag="PKO06_12335"
     CDS             complement(2031..2420)
                     /locus_tag="PKO06_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70479.1"
                     /translation="MKMLRLRGKVEEIEEKILIVSDRKGNETFRLPQSSMPDLEPGDA
                     VDCYVVPPSSPTGLCRVMSRKDKKAEKPKRSKEATTLMRAMLKMRATLIAEQNVLERN
                     ENPDPNELDRLFEKLEFLDRGFKLFAE"
     gene            complement(2604..>2882)
                     /locus_tag="PKO06_12340"
     CDS             complement(2604..>2882)
                     /locus_tag="PKO06_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70480.1"
                     /translation="RVSIRTTGMGRIVPIMDSPGLADAIVDVVRNRESYMRSRAQIEE
                     HFSMGKTVREYERLFEMLIGIVEPATGPCGRRQQLQLHVIVNGAATEP"
BASE COUNT          624 a          825 c          776 g          657 t
ORIGIN      
        1 cttcgaaagc cgtcttctcg tcatctttcc agagatcggt catcgagaaa tgatacgttc
       61 gggcgagcat ctcgaggcgg ccgagatggt gcagatagtg agcccaggcg ttcatcacgc
      121 gccaaatttc accaggtgtc gccagggcgt tttccttggc cactttttca aacaacggaa
      181 tccgcggcgt gaggatccgg gtcatcagtt cgagacgatc ggctgcctcg aaaaacaagg
      241 cctgatctcg ctgcagtgac tgcgtatact ctgaaaaatt ctcgcgagcg gcggcaatga
      301 tcgggtttcg acccgtatgc tcactctgcc ctgtaaagtc gggaagcgtc agagttggct
      361 tggcccgaaa catcagagct gccgcgtctt gaggcaaagc gacaagcgcg acgagcagac
      421 tgatacacag acaaaatcgg ggaacaaagc cccgggttgt tgcgggcaat cgatgaatca
      481 tcatggttct cctgtacttc agggcctgca gtagtgcgga caactgtatt gtctctattc
      541 tgacacaaga ccagacgcgc gacaagagca gataatgaca tgaaaacgta tgaaccatat
      601 cgaatgagat cgagggtgac gaaacgaaaa gttgtgctac catagcgggt gtgaacgacg
      661 ccaagccgaa actgccgtac accgtcgcct tcatgattcc gcccgaagcg atcaatctgc
      721 tggtcttctg gcgcaagaag gtcgctccgt tctgcgaaaa aatcgaccca ctcgaagtcg
      781 cgcacatgac cgtgaagtat ctcggctttc cgtccgagac gtttcgcgaa gaaagcgtgg
      841 tctcgttgat tccgcgtctg gcgaaaatcg tggcgccgca tctgccggtg cgagtctcgg
      901 ttcgcgggtt agacctgttc gattgtcatt gtgagccggt cgtctatctc aaggtcgtct
      961 caggtggcgc actggccgag attcatgacg cgatacgagt cggcctgggc aaggatatcg
     1021 agacctttcc gcatgccgat ggcgagaact accggccgca tatcacgatc tcgaaacaga
     1081 tcaagcacag acagatcgat gccttgcgac agctgattca tcggtcgaaa aaaagcgcca
     1141 aacgcacatt caagctgacg caactggtac tcctgactcc gtgggacatc tatccgatcc
     1201 tgcctctggt cgggcgaaag cacccaccga ccaactggtg agcagatgcc ggctgtactt
     1261 cagttcatct gctggtacaa aactggatac tcaaacggtc tttcgtgtaa gatcttttcg
     1321 taatcccatt cggccttgcg ccgtctgtcg acaaggaggt tgagcgatgt tggtgacgag
     1381 gaattcctgc acgatctggt tgcccgttct cattctggtt ggccttctca cggttgtgcc
     1441 aggtcaggca caatttcaga cagcgcccgc tcctgctccc acgatggacc aggcgaccgg
     1501 tggcgctccc agttcctgct gcggacaaca ctgttccctg agctgcagac atgggtttta
     1561 taccactgac ggaatgaacg tctactggtt tcctcacggg tatcctcgct cgatccaccg
     1621 cgtgcagtcg aacacgaata ccggtcccga cctgcgtttt gccacgcggt atcgctggtc
     1681 ccaccggcac taccacgcgt gcgcctgtca tccggtctat caaatgcagt tcaagggccc
     1741 ctattggaca cacacgacgt accgaccggc acgtggcctg cttcatcgca gtacctggcg
     1801 ctgcacctgg atcccctact atgtggcggc gatcccgaac gcctcggtgc tgggtaccgt
     1861 gccacacact tcaaaccccg ttccgtaaac gcgagttgtg ccaggcctgt tttggccgtg
     1921 gcaccacgaa acgtggctgc cttctgcact ggtcttgagt tcagtctgag cccagaccta
     1981 ctgctcagga cgcttgcccc gtttttctgg aaaagattgt ccgagtcgtt ttactcggca
     2041 aagagcttga agccgcgatc gaggaactct agtttctcga atagtcggtc gagttcgttc
     2101 ggatcgggat tctcgttgcg ctcgagcacg ttctgctccg cgatcaacgt cgcgcgcatc
     2161 ttgagcatgg cacgcatcag ggtggtagct tccttcgacc gttttggttt ctcggctttc
     2221 ttgtccttcc gtgacatcac acgacagagc cccgttgggc ttgatggggg aacgacatag
     2281 cagtctacgg catcgcccgg ttcgagatcg ggcatggacg actgcggcag ccgaaaagtc
     2341 tcattccctt tgcggtcgct cacgatcaga atcttttcct caatctcctc gacctttccg
     2401 cgcagacgca gcatcttcat gcagattcct cccggcaatg atgtcaccat tatggtctga
     2461 attgaaccga ccggcaagcg tgatgtgtct atcgtgagac cattcacggg cgaataaaac
     2521 gtaacagttg acaatatgac agtaactgtt atatttaaga tatggaacag aaaaagtact
     2581 ccattggcga ggttgcgggg ctgtcagggt tcagtcgccg cgccattcac tattacgtgc
     2641 agctgaagct gctgtcgccg cccgcagggg ccggtcgcgg gttccactat accgatgagc
     2701 atctcgaaca gcctttcata ttccctaacc gttttcccca tcgagaaatg ctcttcaatc
     2761 tgtgcccgcg agcgcatata agactccctg ttgcgcacca catccacgat ggcatcggcc
     2821 aggcccgggg agtccatgat gggaacaatc cggcccatgc cagtggtgcg gattgagacg
     2881 cg
//
LOCUS       DAORHD010000574         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40457_length_2880_cov_3.343009, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000574 DAORHD010000000
VERSION     DAORHD010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40457_length_2880_cov_3.343009"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PKO06_12345"
     CDS             <1..531
                     /locus_tag="PKO06_12345"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNV70481.1"
                     /translation="RNYPEKWTTGQGIEQQIDNTVFGLDLHIGIDEYRMTQRSVKYAV
                     LFLVLLFAVFWLFEIKARVGVHPMSYLFIGVAICLFYLLELSLAEHIGFDAAYIVASA
                     MVIVLVSGYSASVLASFGRAIVVAIVLGLLYAYLHVVLVNQDYALLLGSLGLFAMLAI
                     VMFVTRGINWEGDLNS"
     gene            531..1820
                     /locus_tag="PKO06_12350"
     CDS             531..1820
                     /locus_tag="PKO06_12350"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HNV70482.1"
                     /translation="MTTNTEAAVAVLNHPFLAGSIFLVTFVLVSFRQMRILPIGRPAA
                     AMLGAVSMVVGGVMTPEQAYTLVNWDTIGLLLGMMIIAEYLRDAGFFRSLTRRLHRMQ
                     SPFRLLAAISLFSGLASAFLVNDTICVVMTPLVLGICESRRLHPFPYLMALATSSNIG
                     SALALTGNPQNMIIGVLSGLDYTRFLVLMLLPVAVSLVLNIVLLRWFYGPMLKSPLPE
                     DAVTPSPVLDDDETLGKPIARLSFSVLALIAACVGFFSGFSMAFSALAGASLVILLNR
                     KDPKEFLARVDWPLLLFFSSLFIVIGGLKVSGLSAAGTAWAMQFVSGTLQHQSWVFSL
                     VTLIGSNVFSNVPFVMLVGQTVPHLAAPQLFWSLLAFVSTVAGNLTLFGSVANLIVAE
                     GARGRCEVGFMAYARFGVPSTLLVVSIGTAILLALLG"
     gene            1981..2640
                     /locus_tag="PKO06_12355"
     CDS             1981..2640
                     /locus_tag="PKO06_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70483.1"
                     /translation="MQRSLSQLLIIALVLGIGFAIPGTGHAAPGDPQKIVPGMPFHFF
                     DLEDLNRQRWVSTYLRGRPVILITGHRDQKYDMLKWADALKREFADPGMAHVLWVMNF
                     RKLSSGTSRKTVTDMWRAFAPPVPMLLDWHGVVGRGLKINYGVPNVIAIDAWGRLAFH
                     EMHSFTPEVYAAVSSYIRPLLVGNPDFPKAAIETPIEGKMLDNQLPYGATPKGKKGYS
                     Y"
     gene            2685..>2880
                     /locus_tag="PKO06_12360"
     CDS             2685..>2880
                     /locus_tag="PKO06_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009435363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV70484.1"
                     /translation="MKINRLCLLALVCIVMLTGFTPAGAQEARPHIMVVNDDGITSDG
                     IMALVRALETFADVTVCAPTQ"
BASE COUNT          482 a          839 c          837 g          722 t
ORIGIN      
        1 aggaactatc ccgagaaatg gaccacgggc cagggaatcg aacaacagat cgacaacacc
       61 gtcttcgggc tcgacctgca catcggcatc gatgaatacc ggatgaccca gagaagcgtg
      121 aagtacgccg tccttttcct cgtgcttctt tttgcggttt tctggctgtt tgagatcaaa
      181 gcgcgcgtgg gcgttcaccc gatgagttat ctattcatcg gcgtcgccat ctgcctgttc
      241 taccttctcg aactttctct ggcagaacat atcggcttcg atgcggccta tatcgtggca
      301 tcagccatgg ttatcgttct cgtttccggg tacagtgcca gtgttctcgc gtcattcggg
      361 cgggcgatcg tcgtcgcgat tgtgctcggg ttactctatg cctacctgca tgtggtgctg
      421 gtgaaccagg attatgctct cctgttgggc tctctcggcc tgtttgcgat gctggccatc
      481 gtcatgttcg tcacccgggg aatcaactgg gagggcgacc tcaacagctg atgaccacga
      541 atactgaagc ggccgtcgcc gtactgaacc atccgttcct ggcgggttcg atctttctcg
      601 tgaccttcgt gctggtctca tttcggcaga tgcgcattct gccgatcggc cgcccggcgg
      661 cggccatgct cggcgcggtc tcgatggtcg tcggcggcgt catgacccct gagcaggcct
      721 atactctggt caactgggat accatcggcc tgttgctcgg gatgatgatc atcgccgagt
      781 atctgcgtga cgcggggttc ttccgttctc tgactcggcg tctgcaccgc atgcagtccc
      841 cgtttcgact tctcgcggcg atcagtctgt tttccgggct ggcttcggcg ttcctcgtga
      901 atgatacgat ctgcgtggtc atgactccgc tggttctcgg catctgcgaa agtcgccgcc
      961 tgcacccgtt tccatatctg atggccctgg ccacctcatc gaacatcggc tcggccctgg
     1021 cgttgaccgg gaacccgcag aacatgatca tcggcgtgct ttcaggtctc gattacacgc
     1081 ggttcctggt gctgatgctg cttcccgtcg cggtgtcact ggttctgaat atcgttctgc
     1141 tgcgctggtt ctacgggccg atgctgaagt cgcctctgcc ggaagacgcc gtcacgccga
     1201 gccccgttct cgatgacgac gagacgcttg gcaagccgat tgcccgcctg tcgttcagcg
     1261 tgctggccct tatcgccgcc tgcgttggct ttttctctgg cttttccatg gctttttccg
     1321 cgctggccgg cgcttcactg gtcattctgc tcaatcgcaa ggatccgaag gagtttctcg
     1381 ccagggtcga ctggccgctg ctgctgttct tctcttcgct gttcatcgtc atcggcgggt
     1441 tgaaggtcag cggtctgtca gccgcgggaa cggcctgggc catgcagttc gtcagcggaa
     1501 ccctgcaaca ccagagctgg gtgttttctc tggtgaccct gatcggttcg aacgtgttct
     1561 cgaatgtgcc tttcgtcatg cttgtcgggc agacggtacc gcatctggcc gctcctcagt
     1621 tgttctggag tctgctggcc ttcgtcagca ccgttgccgg aaacctgacg ctcttcggtt
     1681 cagtcgccaa tctgatcgtg gccgagggag ctcgcggtcg ttgcgaagtt ggtttcatgg
     1741 cctatgcccg gttcggtgtc ccttcgacgc tgcttgtcgt cagtatcggt accgcgattc
     1801 tgctcgccct gctcggatag acgaacgagg cgatcagggg cgggccgggt tgaccgggcg
     1861 gtcctgttcc gatgatctcg tctgtttttt tcacgttcgg cgtttgcgca gggggctttt
     1921 tgtggtattt ttcgaactga gttcgaaatc gaacattgtg accgtcacgg aggaatgagt
     1981 atgcagagat cgctttcaca acttctgatc atcgccctgg tgctgggcat cgggtttgcc
     2041 atccccggta cgggccatgc agcccccggt gatccgcaga agatcgtgcc cggtatgccc
     2101 tttcactttt tcgatctcga agacctgaat cggcagcgct gggtcagcac ctatctgcgt
     2161 ggccgccccg tcatcctgat taccggccat cgcgatcaga agtacgacat gctgaagtgg
     2221 gccgatgcgc tcaaacgcga attcgccgat cccggcatgg cgcatgtgct ctgggtcatg
     2281 aacttccgca aactgtcttc cggcaccagt cgcaagaccg tgaccgacat gtggcgcgca
     2341 tttgcgccgc cggttccgat gctgctcgac tggcatgggg tggtcggtcg cgggctgaag
     2401 atcaactacg gcgtgccgaa cgtcatcgcg atcgacgcct gggggcgtct ggcgtttcac
     2461 gaaatgcact cgttcacacc ggaagtctat gccgctgtct ccagctacat cagacctttg
     2521 cttgtcggca acccggactt tccgaaagca gccatcgaga ccccgattga gggaaaaatg
     2581 ctcgacaacc agcttcccta cggtgctacg ccgaaaggaa agaaaggcta ctcgtactaa
     2641 acgaagttga tgaagtgaaa cagagaattc agaaaggaaa aggaatgaag attaatcgtc
     2701 tgtgtctgtt ggctttggta tgtattgtga tgctgaccgg ttttaccccg gccggcgctc
     2761 aggaagcgcg accgcatatc atggttgtga acgatgacgg catcacgtct gacggcatca
     2821 tggctctggt gcgcgcactc gagaccttcg ccgatgtgac ggtctgcgct ccgacgcaga
//
LOCUS       DAORHD010000575         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40590_length_2874_cov_2.600922, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000575 DAORHD010000000
VERSION     DAORHD010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40590_length_2874_cov_2.600922"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKO06_12365"
     CDS             complement(<1..706)
                     /locus_tag="PKO06_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70485.1"
                     /translation="MRTLLALVWLSLALPLWSGPLAEKAATFDRVIQQKHCPEGLVFD
                     VHLDDQGNFRKIRGGGDSLIWSGAYAASQVYRYRVTKDPLALQNVEKTLWAFHHLQAM
                     SGSPGFVGRSFVDPAWIDHVGNRLPGVGTYSKRLFQPSSSRDQYTGLFLAYALAWPII
                     TDPALRATVREDVRAIGRSLVENRLAMIGRFPTGEERAFNVDPCYIYQDRLTREEWEK
                     VDDFPANVLAKLCPYDE"
     gene            complement(710..1102)
                     /locus_tag="PKO06_12370"
     CDS             complement(710..1102)
                     /locus_tag="PKO06_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV70486.1"
                     /translation="MASDNPFLHGNQWQDFIKKYKTNEVIFWEGDAADLFFIVLEGRV
                     QIKRSRPNGNSLILASLGPGQFFGEMALMSNSPRTATAFAEEQTSLLGMNQKQFLDML
                     QDNNQFALKMIRTLCERLKVLGDQLVHV"
     gene            complement(1191..1847)
                     /locus_tag="PKO06_12375"
     CDS             complement(1191..1847)
                     /locus_tag="PKO06_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70487.1"
                     /translation="MKKLFLVLVLVCGLLFPALPVWSQEDSSRVITDLPGAYEIWKTR
                     GIKFVIRENGQFVTWAEGRLESWSGKSKWVVRDPKGKFLTHAFGRIETWKTGKTRLVL
                     RTPKGQIMTHIDINLTNKASFAQNVVSLRRLEQSRFLSFAQETIGELLIQDLKQKDTL
                     RTGVLIKYLTLKKGEPGLSNFKPVLRMILPQLNFVASHSQEPKVLEMADKARELLKTI
                     "
     gene            complement(1900..2166)
                     /locus_tag="PKO06_12380"
     CDS             complement(1900..2166)
                     /locus_tag="PKO06_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70488.1"
                     /translation="MISDTDRKDLFDRLGKEEDAKVRYLQGLVDSAVLKIMNSRLTRN
                     QALQVVLEVRAVAAELFPNDMETFDLIYMGRFKRTIDEHCREDE"
     gene            complement(2231..2815)
                     /locus_tag="PKO06_12385"
     CDS             complement(2231..2815)
                     /locus_tag="PKO06_12385"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV70489.1"
                     /translation="MKKRTGFLALIGFAALALFAAPAIVLAEETPLPASSKVTIGGET
                     PLEAEVELKIGDTAPDFSLPNLVTDKMESLKDYLGKPTVLFFIQSACYSCLQEAKALK
                     TLKEQYADINVLAVGVDLLGKPMLVSWATHNSLNYPVLLDPIFSVPEKYGFSYTPSSV
                     IIDKDGKIAWIHAGFKPTDIQLFEDKIKELLGKK"
BASE COUNT          637 a          804 c          792 g          641 t
ORIGIN      
        1 cctcgtcata cggacagagt ttcgccagca cgttggccgg gaaatcatcg accttttccc
       61 attcctcgcg ggtcagtctg tcctggtaga tgtaacacgg atcaacgttg aatgcccgct
      121 cttcaccggt tggaaatcga ccgatcatgg ccagacggtt ttcaaccagg gaacgaccga
      181 tcgcccgcac atcctcgcga acggttgccc tcaaagccgg gtcagtgatg atcggccagg
      241 ccaaggcata cgccagaaac aacccggtgt actgatcacg tgaagaagag ggttgaaaca
      301 atcgtttcga gtaggtaccc acgccgggca gacgattgcc gacgtggtcg atccaggcgg
      361 ggtcgacgaa cgatcgcccc acgaagcccg gagacccgct catcgcctgc agatggtgaa
      421 aagcccacag ggtcttttcc acattctgca gcgccagggg gtctttcgtg acccggtagc
      481 ggtacacctg cgaggcggcg taagcgccgc tccagatcaa cgaatcaccg ccgccccgga
      541 ttttcctgaa gttcccttgg tcgtcgagat gcacatcgaa aaccagacct tcggggcaat
      601 gcttctgttg aatcacgcga tcgaacgtcg cggctttctc ggcgagcggg ccgctccata
      661 aaggcagagc caggctcagc caaacgagtg caagaagagt tctcatctct tagacgtgaa
      721 caagctgatc accaagcact ttgagccgct cgcacagggt acggatcatc ttgagcgcaa
      781 actggttgtt gtcctgcaac atgtcgagaa actgtttctg gttcatgccc agcaacgatg
      841 tctgctcttc ggcgaaggcc gtcgccgtgc gcggagagtt gctcatcagc gccatttcac
      901 caaagaactg acctgggccg agggaagcga gaatcaacga attgccgttc ggccgactgc
      961 gcttgatctg cacgcgacct tcgagcacga taaagaacag atcggcggca tcgccttccc
     1021 agaatatgac ctcgttggtc ttatattttt taatgaaatc ctgccactgg ttgccatgca
     1081 gaaacgggtt gtcagatgcc atgtctctca ctcctcctct tcagctaacg cctatgctct
     1141 tcatgaaaac gccgcccggt gtgagaccga gcggcgttct gccagttttt tcagatggtc
     1201 ttgagcagct cgcgagcctt gtcggccatt tccagaacct tgggttcctg agaatgcgac
     1261 gcgacgaagt tcaactgcgg cagaatcatg cgcagtaccg gcttgaaatt cgacagcccg
     1321 ggttcacctt tcttcagcgt cagatacttg atcagaacgc ccgtacgcag cgtgtctttc
     1381 tgcttcagat cctgaatcag cagttcgccg atggtttcct gagcaaaact caagaagcgg
     1441 gactgttcga gccgacgcag gctgaccacg ttctgagcaa acgaagcttt attggtcagg
     1501 ttgatgtcga tatgggtcat gatctgaccc ttcggtgtgc gcaataccag gcgagtcttg
     1561 ccggttttcc aggtttcgat gcgaccgaag gcgtgcgtca gaaacttgcc cttcgggtca
     1621 cgaaccaccc acttcgactt gccagaccac gattcaaggc ggccttcggc ccaggtgacg
     1681 aactggccgt tttcgcgaat gacgaatttg atgccgcggg ttttccagat ttcgtaagcg
     1741 ccgggcaggt cggtgatgac cctgctcgag tcttcctgtg accagactgg aagcgccggg
     1801 aacaacagac cgcataccag aacgaggacc agaaacagtt ttttcatcgt gggttgtctc
     1861 cttgccagag gaataggctg atcaattcta ccacatggct cattcatctt ctcgacaatg
     1921 ttcgtcgatg gttcgcttga agcggcccat atagatcaga tcgaaggttt ccatgtcgtt
     1981 agggaacagt tcggcggcaa cggcacgcac ctcgagaacc acctgcaggg cctggttacg
     2041 ggtcaggcgc gagttcatga tcttgagaac ggccgaatcg accaacccct gcagataacg
     2101 cactttcgcg tcttcttcct tgccgaggcg gtcgaagaga tctttgcgat cggtgtcaga
     2161 aatcatgaaa cgaaacgccc ttgaaaaaaa caccccgcga gtgccttcac tcgcggggtg
     2221 tttgctcgtc ctacttcttg cccagcagtt ccttgatctt gtcctcgaac aactgaatgt
     2281 cggtcggctt aaaaccggcg tgaatccagg cgatcttgcc atccttgtcg atgataaccg
     2341 agctgggggt gtatgagaaa ccgtattttt caggtacgga aaagatcgga tcgaggagca
     2401 cgggatagtt caagctgttg tgtgtcgccc aggagacgag catcggcttg ccgagcagat
     2461 cgacgccgac ggccagcacg ttgatgtcgg cgtactgttc tttcagggtc ttgagagctt
     2521 tcgcttcctg caggcaggag tagcaggcgc tctgaatgaa gaacagaacg gttggtttgc
     2581 ccagataatc cttcaggctt tccatcttgt cggtcacgag gttgggaagc gagaagtcag
     2641 gtgccgtatc gccgatcttc agttcaactt cagcttcgag cggcgtttca ccgccgatgg
     2701 tcaccttgct gctggcaggc agcggagtct cttcagcgag tacgatggcc ggagccgcga
     2761 acagcgcaag ggccgcaaaa ccgatcagag cgagaaagcc ggttctcttc ttcatgtgaa
     2821 acctactccc ttcgggaaag tgatcagatt ctagcacacg ccccggaacg ggtc
//
LOCUS       DAORHD010000576         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40698_length_2869_cov_2.632907, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000576 DAORHD010000000
VERSION     DAORHD010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40698_length_2869_cov_2.632907"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKO06_12390"
     CDS             complement(<1..249)
                     /locus_tag="PKO06_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV70490.1"
                     /translation="MTFSRLTIACAFSVFGLYLLLVTGLLGFTDPTRFLTILGTDRVL
                     YSIRLSVSAALLATFLSMCFAIPAGYALSRYEFRGKKLV"
     gene            complement(281..1129)
                     /gene="modA"
                     /locus_tag="PKO06_12395"
     CDS             complement(281..1129)
                     /gene="modA"
                     /locus_tag="PKO06_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV70491.1"
                     /translation="MIRTFLLLCVLCLSLSAPATAAEHLLFMAGAAAQPVVEELVQGF
                     EAKTGVKIDANVGGSGSLLSQIKLSKSGDVYFPGSIDFINRGIQDDQIVASSVVPIVY
                     LVPALNVQPGNPKKITNFKDLCRPDVKVIIANPETVCLGVFAVEMAEKLLTPDEKKAF
                     RANIVNYAESCEKTANSIALKAADAVVGWSVFQHWNSKLIETVPLPPEQILRISYLAI
                     SMTKYAKNPETAQKFIDYVKSAEGMDVFKKYRYFVTAEEAQAYIGQPKPIGGDCFVVP
                     EEWMSK"
     gene            1439..1876
                     /locus_tag="PKO06_12400"
     CDS             1439..1876
                     /locus_tag="PKO06_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70492.1"
                     /translation="MSKLDSISLLVRRGTQWFERLLAVCIFLGVIVFTFKSAIAFSQM
                     DWASTEAIYELVYRVLLAVIGLELIRTLLTHNLQSVLELLAFVVARKTLKPDLSVVDI
                     LLSVLAFAILLLCRRYLVVCPPEDDAGADALNTATGGTSSHSG"
     gene            2174..2866
                     /locus_tag="PKO06_12405"
     CDS             2174..2866
                     /locus_tag="PKO06_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HNV70493.1"
                     /translation="MSQKKPWTETEEPTGTTALTASEPTLRRLPVYHHYLQSILDQGR
                     DVISCTHIATDLKLDPTQVRKDLATTGIVGKPKVGYEISVLLETIESFLGWNNTHEAF
                     LVGVGHLGAALLGYSGFSRYGLKIIAAFDTDKAKIGTSLHGIEIRPLTKLENLARRLR
                     VRMAVMTVPAAEAQAVADLLSESGIEAIWNFAPVQIQVPDSVIVQHENLAAGLSILSA
                     KLTQLKPRVRSP"
BASE COUNT          596 a          827 c          847 g          599 t
ORIGIN      
        1 gacgagcttc tttccccgaa actcgtaacg cgacagggca tacccggcgg gaatggcgaa
       61 acacatggaa aggaaggtcg ccagcagagc cgcgctcacg ctcagcctga tcgaatacag
      121 cacccggtcc gtgccgagaa tggtcagaaa gcgcgtcggg tcggtgaatc cgagcaggcc
      181 ggtcacgagc agcagataca gcccgaagac cgagaatgcg caggcgatcg tgagtcgtga
      241 aaacgtcacg tgacgccctc tgcgcgctgc cgactctgat tcatttgctc atccactctt
      301 ccggaaccac aaagcagtcg cccccgatcg gtttcggctg tccgatatac gcctgagctt
      361 cttcggcggt gacaaaatag cggtacttct tgaacacgtc cattccttcg gcagatttca
      421 cgtagtcgat gaacttctgc gcggtctcag ggttcttggc atacttggtc atcgagatcg
      481 cgagatagct gatgcgcaga atctgttcgg gcggcaacgg cacggtctcg atcagtttgc
      541 tgttccagtg ttgaaagacg ctccagccga ccacggcatc ggctgccttg agagcgatac
      601 tgttcgcggt cttttcgcaa ctttcggcgt agttcacgat gttggcgcga aacgcctttt
      661 tctcgtcggg ggtgagaagc ttctcagcca tctcgaccgc aaagacaccc aggcagacgg
      721 tttccgggtt cgcgatgatc actttgacat cgggacggca gagatccttg aaattggtga
      781 tctttttcgg gtttccgggt tgaacgttga gggccggtac cagatagacg atgggaacca
      841 ccgacgaggc gacgatctgg tcatcctgga tcccgcggtt gatgaagtcg atcgagcccg
      901 gaaagtagac atcgccgctc ttcgacagct tgatctgtga caacaggctc cctgagccgc
      961 cgacattcgc gtcgatcttc accccggtct tcgcctcgaa gccttggaca agttcctcga
     1021 cgaccggttg agcggcggca ccagccatga acagcagatg ctctgccgcg gtcgcgggcg
     1081 cactgaggct caggcacaga acacacagaa gcagaaaagt gcgtatcacg aaagtcctcc
     1141 ttcgccctcg tcgtgaatcc atgcgaaggc gcattagata gattcagata ttgtatagta
     1201 gcaattgctt tgctataagc ataatgctta cccacactct gtgtcaagga accattccgc
     1261 catcgtgacg ggggcgcgca ccttcctgga gacataatgc ccggcagaag gcggtgcgca
     1321 accggtcgtt gacgcgaggc cgattccggg gtggggtcag aaaaccgctg agacagcgtg
     1381 gttccggcga cacgcgcgcg ggtttttcgt tcgggtgccg atgtggtatc gttcggatat
     1441 gagtaaactc gattcgattt cgttgctggt tcgacgtggc acgcagtggt tcgagcgtct
     1501 gctcgcggtc tgcatctttc tcggcgtcat cgtctttacc tttaaatcgg cgatcgcctt
     1561 cagccagatg gactgggcca gtaccgaagc catctacgaa ctggtctacc gggttcttct
     1621 ggccgtgatc ggtctcgagc tgattcgtac gctgctgacg cacaatctgc agtccgtgct
     1681 cgaacttctc gccttcgtcg tggcgcgcaa gacgctgaaa ccggatctct cggtcgtcga
     1741 tattctgctg agcgttctgg cttttgcgat tctgctcctc tgccgacgct acctggtggt
     1801 ttgcccacct gaagacgacg cgggtgctga tgctctgaat accgccacag gaggcacttc
     1861 gagccactcg gggtaagtgc ccctgatgaa cccgacaaag accacggtgg cgaggccgca
     1921 cctgaaaaac agattcctgc cgggtgaggt gcaggccgcc gtggcgcaga aatcccgaaa
     1981 cgtcactgcc gtagcgcctt tccgtcgggc gctaagaagg gctccttccg cgtgaacctg
     2041 cctcgtcggc aacggcgggt aaatcgagaa aaatatctcg aaagggcttg actcgagaaa
     2101 gatttctcga tatatttgaa tcgagaaata aatctcgatt atcacgacga cgcacagagc
     2161 gcggaggttc gcgatgtcac agaagaagcc atggacggag acggaggaac cgacggggac
     2221 gacggcgctg accgcgtctg aaccgaccct gcggcggctt ccggtctacc atcactatct
     2281 acagagcatt ctcgatcagg ggcgcgatgt catctcgtgc acgcatatcg cgaccgacct
     2341 gaagctcgac ccgacgcagg tgcgcaagga cctggccacg accggaatcg tcggcaagcc
     2401 gaaggtcggc tacgagatct cggtgctgct cgaaaccatc gagtcgtttc tcgggtggaa
     2461 caacacgcat gaggcctttc tggtcggcgt cggtcatctg ggcgcagcgc tgctggggta
     2521 ttccggcttt tcgcgctacg gtctgaagat tatcgcggcg tttgacacgg acaaggccaa
     2581 gatcggaacc tcgctgcatg gcatcgagat tcgtccgctg accaagctcg agaatctggc
     2641 ccggcggctg cgcgtgcgca tggcagtcat gaccgtgccg gcggccgaag cgcaggctgt
     2701 ggctgatctg ctgagcgaat ccggtatcga ggcgatctgg aacttcgcgc cagtgcagat
     2761 tcaggtgccg gattccgtca ttgtgcaaca cgaaaacctg gcggccggac tctcgattct
     2821 ctcggccaaa ctgacgcagc tcaaaccccg cgtccgcagc ccctaacgg
//
LOCUS       DAORHD010000577         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40710_length_2868_cov_4.034838, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000577 DAORHD010000000
VERSION     DAORHD010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40710_length_2868_cov_4.034838"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..469)
                     /gene="uppS"
                     /locus_tag="PKO06_12410"
     CDS             complement(8..469)
                     /gene="uppS"
                     /locus_tag="PKO06_12410"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNV70494.1"
                     /translation="MIQQTSVPSEFKSREEYREHLRQQVLKARVPVHVGLIMDGNGRW
                     AKERGYSRIRGHQMGARSIKRLVRFADKLGVKHISLYVFSSENWSRPRLEVVGLMKLI
                     RAYVIRERDELHAEGVRFRHLGRREELPSGLAQDLDIAEKLTEKLTAPITK"
     gene            complement(466..660)
                     /locus_tag="PKO06_12415"
     CDS             complement(466..660)
                     /locus_tag="PKO06_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV70495.1"
                     /translation="MYRITEDCVTCGSCKPVCPVGAIVEDDPYVITSKCTDCGKCAEV
                     CPVEAIVPGSKTPQRKSTRS"
     gene            complement(665..1225)
                     /gene="frr"
                     /locus_tag="PKO06_12420"
     CDS             complement(665..1225)
                     /gene="frr"
                     /locus_tag="PKO06_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNV70496.1"
                     /translation="MSVEKIMKDIEAKMEQTVEVMRKEFSTMRTSRATPSLLDRISVS
                     YYGTETPLHHLASISAPDPKTLVIAPHDKTVLGDIEKAIQKSDLGLNPQNDGRIIRLP
                     VPALTEERRNDLVKTCKKKAEEFRVAIRNLRRDANDAVKAVEKKSEITQDESKKAIER
                     IQKTTDAYIEKLNKQLESKEHEILGK"
     gene            complement(1241..1969)
                     /gene="pyrH"
                     /locus_tag="PKO06_12425"
     CDS             complement(1241..1969)
                     /gene="pyrH"
                     /locus_tag="PKO06_12425"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNV70497.1"
                     /translation="MQQNQLVYRKPLLKLSGEVFGGETGFGFHRQTLETYAQNIAALV
                     KSGIIPSLVIGGGNFFRGARGHLPAMQRHHADSIGMLATLMNALCFADHLRAIGVPCD
                     VFSAIAMPQVCRTYIIDDVIAALQAGKVCLFGCGTGHPYFSTDTGAALRAIEVGCDVL
                     IKATKVDGVYDSDPAKNPEARKFDWITYKEVLERNLGVMDMGAISLCRENRLPLLILS
                     MNEKDSILRACRGENIGSRVMEDS"
     gene            complement(2051..>2868)
                     /gene="tsf"
                     /locus_tag="PKO06_12430"
     CDS             complement(2051..>2868)
                     /gene="tsf"
                     /locus_tag="PKO06_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNV70498.1"
                     /translation="LASADGKADRKASMGLVTLMVAADKRSASIIEANCETDFVAKNE
                     NFISFAQTLAELQLSDKNIKTPADLTAQKTKDGQPVEEMRKLLIAKIGENMLVGRTER
                     LEIPSGHHGLIDLYVHGDKEITKTGTLGKNQFGMGAIGVLVCVEAANPDAVKHDDLQH
                     FAHEVALQACAMKARFLSRDQVPADVVAREKDVILGQIKEDPKNANKPENVLAKIVEG
                     RLDKFYKERCLLEQIYVKDDTKTIKDLMNDMGKKMGGEVKITAFRRWALGEQG"
BASE COUNT          569 a          814 c          785 g          700 t
ORIGIN      
        1 gtgcctttca tttggttatc ggagcggtga gtttttcggt cagcttctca gcgatatcga
       61 gatcctgtgc caatcccgag ggcagttcct cacggcgtcc caggtggcgg aagcgcactc
      121 cttccgcatg cagttcatcg cgttcgcgaa tgacataggc acgtatgagc ttcatcaggc
      181 cgacaacttc gaggcgtggg cgactccagt tctcggaaga aaagacatac agcgagatgt
      241 gcttgacgcc aagtttgtcc gcaaagcgaa ccagtcgttt gatactgcga gcgcccatct
      301 gatgtccacg aatgcgactg tacccgcgct ctttcgccca acgcccgttc ccatccatga
      361 tgaggccgac atgaactggg acgcgggctt tgagcacctg ctgacgcagg tgctctcgat
      421 actcttcacg actcttgaat tcagacggaa cgcttgtttg ctgtatcatg aacgggtact
      481 tttgcgttgc ggcgttttcg agcccggcac gatagcctcg accggacaga cctcagcaca
      541 tttgccacag tcagtacact ttgaagtgat cacgtacgga tcatcttcca caatggcccc
      601 aaccgggcag acgggcttgc atgatccgca ggtcacacaa tcttcggtga tccgatacac
      661 gcggctattt ccccagaatc tcgtgttctt tcgactcgag ctgcttgttc agcttctcga
      721 tgtaggcgtc ggtggtcttc tgaatgcgct caatggcctt cttgctctca tcctgcgtga
      781 tctcgctctt tttctcgacg gctttaaccg catcgttggc atcgcggcgc agattgcgga
      841 tggcgacccg gaattcctcg gccttctttt tgcaggtctt caccaggtcg ttgcggcgtt
      901 cttcggtgag agccggcacg ggcagccgaa tgatacggcc gtcgttctgg gggttcaagc
      961 ccagatctga cttctgaatc gccttctcga tgtctcccag cacggttttg tcgtgcgggg
     1021 caatcaccag ggtcttcgga tcaggtgcgg aaatcgaggc cagatgatgc agcggtgttt
     1081 ccgtgccgta gtaactgaca gaaatgcgat cgagcagcga gggggtcgct cgtgacgtgc
     1141 gcatcgtgga aaactccttg cgcatgactt ccacggtctg ttccatcttc gcttcgatgt
     1201 ctttcatgat cttttcaact gacatgattg ttcacctcgt tcaagaatct tccatgacgc
     1261 gtgagccaat gttctcgccg cgacatgcac gtagaatact gtctttttcg ttcatggaca
     1321 agatcagaag tggaagtcga ttttcgcgac acagcgaaat cgcgcccatg tccatgacgc
     1381 ccagattgcg ttccagaact tctttatacg taatccagtc aaatttgcga gcttccggat
     1441 tcttcgccgg atcagagtcg tagacgccgt cgactttggt ggccttgatc aacacgtcac
     1501 atcccacttc gatcgctcgc agagccgctc ctgtatctgt gctgaaatag gggtgaccgg
     1561 taccgcagcc gaacagacag acttttcccg cctgcagcgc ggcaatgacg tcatcaataa
     1621 tgtaggtgcg gcacacttgc ggcatggcaa tggcggaaaa gacatcgcag ggaacgccga
     1681 ttgcccgcag gtgatcagca aagcacaacg cgttcatcag cgtcgccagc atgccgatcg
     1741 aatctgcatg atgccgctgc atggctggca gatgcccgcg cgccccgcgg aaaaaattgc
     1801 ccccgccaat gaccagcgaa ggtataatgc cggatttgac gagcgcggca atattctgcg
     1861 catacgtttc gagggtctgc cggtgaaacc caaagcccgt ctcgccgcca aacacctcgc
     1921 cgctcagttt cagcagaggc ttacgataaa ccagttgatt ctgttgcatg cgtcctcaca
     1981 gacctccgaa tgtagcaaaa gccgcgagag gaaaaccctc tcgcggcttt tgtcatgaac
     2041 cggaaaggaa tcagccctgt tcgccgagag cccagcggcg gaacgccgtg atcttcacct
     2101 caccacccat cttcttgccc atgtcattca tgagatcttt gatggtcttg gtgtcatctt
     2161 tgacgtagat ctgctcgagc aggcagcgct ctttgtagaa cttgtcgagg cggccttcga
     2221 cgatcttggc gagaacgttt tcaggtttgt tggcgttttt cggatcttcc ttgatctgac
     2281 ccaggatcac gtctttttcg cgggcgacga catcggccgg cacctgatcg cgggagagga
     2341 agcgggcctt catggcgcag gcctgcaacg caacttcgtg agcgaagtgc tgaagatcgt
     2401 cgtgtttgac ggcatcaggg ttcgcggctt caacgcacac gagaacgccg atggcgccca
     2461 tcccgaactg attcttgccc agagtgccgg ttttggtgat ttctttgtcg ccgtgcacat
     2521 acagatcgat cagtccatga tggcccgagg gaatttcgag gcgctcggtg cgaccgacca
     2581 gcatgttttc accgatcttg gcaatcaaca gtttgcgcat ttcctcgacc ggctgaccat
     2641 ctttggtctt ctgggcggtc aggtcagcgg gcgtcttgat attcttgtcg ctcagctgca
     2701 gttcggccag cgtctgcgcg aacgagatga agttttcgtt tttggccacg aaatcggttt
     2761 cgcagttggc ttcgataatg gacgcggagc gcttgtcggc cgcgaccatg agtgtcacca
     2821 gacccatcga ggctttgcgg tcggctttgc cgtcagcgct ggcgaggc
//
LOCUS       DAORHD010000578         2865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40788_length_2865_cov_3.012811, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000578 DAORHD010000000
VERSION     DAORHD010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40788_length_2865_cov_3.012811"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PKO06_12435"
     CDS             <1..323
                     /locus_tag="PKO06_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNV70499.1"
                     /translation="QSKAPVAFLELSTVNMDFSWHRSFRGPRPDAYERLILDAMHGDS
                     SLFLRNDEIDGAWGFIDPITTYWNGHPDTPLFLYESGSDGPEAAQQLLTRDQRRWHTL
                     QAHS"
     gene            341..1075
                     /gene="pgl"
                     /locus_tag="PKO06_12440"
     CDS             341..1075
                     /gene="pgl"
                     /locus_tag="PKO06_12440"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HNV70500.1"
                     /translation="MSPATIKVFPDPEFATSAALALAEHLCACPPGHSRWIGLSGGET
                     PRPVYTALRNNLQANPLPWRTCTWFQVDERCVPPQQSTSNQRLIRDTLFPTSDQQPWG
                     TFFSIQHPERIQPAADEYNSQLANLFSASAFTRHTSLILGVGADGHTASLFPDTDWRS
                     AVRNEWYTAIQVPHLNAWRLSLTLHALMQADDLVFLVTGSAKQKILCAMCAPQPDLSL
                     PAVYLATRHASVTMLIDRSAAGALQP"
     gene            complement(1174..1845)
                     /locus_tag="PKO06_12445"
     CDS             complement(1174..1845)
                     /locus_tag="PKO06_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNV70501.1"
                     /translation="MYLSLLVLAMLLPAQASFAKGTFSKITDWFSKKSRSTTVTGGID
                     AIDGRKIMFKTEDGQVLELTGRKAEQLAEHRGAKMRIFGNVRKPDATFPSGAIDVRNF
                     RIVEEKPAEPVAEPAPAVDTAPAPEPTPEPAPEPEPAPEPAPEPEVAPEPTPEPIDVN
                     AIPGEVHEESAGKASTYVVEKGDTLAKISKKVYGTTKNWKKIADANNITNPKALKVGK
                     TLQIP"
     gene            complement(1994..2230)
                     /locus_tag="PKO06_12450"
     CDS             complement(1994..2230)
                     /locus_tag="PKO06_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70502.1"
                     /translation="MNTLRKCKTCGAVATEDGHLCDPTPTKKVYTCEFCGAKAGEERH
                     LCKPMVAQVRYFCQGCGRVSTEEKKLCRPKELEG"
     gene            2444..>2865
                     /locus_tag="PKO06_12455"
     CDS             2444..>2865
                     /locus_tag="PKO06_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70503.1"
                     /translation="MEKLSGSVHQRALEWSARFRSESLTLVDYLDRGKARQTRSTNLE
                     RDFLALSPFGQLQQVFAQAKRAESTTSSLNLKFTLPTADLADPAKFAEWEKSFDLVLR
                     MMAKDDADYQRLKDQFSEMFEITRGHFTGAENSLAQGST"
BASE COUNT          579 a          862 c          749 g          675 t
ORIGIN      
        1 ttcagagcaa agcgccagtt gcattcctgg agctttccac cgtgaacatg gacttttcct
       61 ggcatcggtc ttttcgcggc ccacgtccag acgcctatga gcggctgatt ctcgacgcca
      121 tgcacggcga cagcagcttg ttccttcgca acgatgagat cgatggtgcg tgggggttca
      181 ttgatccgat caccacctac tggaacggtc atccagacac gcccctcttc ctatacgagt
      241 ccgggtctga tggtcccgaa gccgcgcagc agcttctgac ccgtgatcag cgtcgctggc
      301 acacgctgca ggcacactcc taagaagatc cagcctcctg atgtcgccgg ctaccatcaa
      361 agtctttcct gatcccgagt ttgccacgtc agcagctctc gctctcgcgg aacacctgtg
      421 cgcctgccct cccggacaca gtcgatggat cggcttgtcc ggcggtgaaa ccccacgacc
      481 ggtctatacc gcattgagga acaatctcca ggcgaaccct ctcccatggc gcacctgcac
      541 ctggtttcag gttgacgaac gatgcgtgcc gccccagcag tcgaccagca atcagcgatt
      601 gattcgcgac accctgtttc caaccagcga tcagcaaccc tggggaacat ttttctcaat
      661 ccagcatccc gagcgaattc aaccagctgc cgacgagtac aattcacaac tcgccaacct
      721 tttctcggcg agcgcgttca cgcggcacac ctcgttgatt ctcggtgtgg gcgcggacgg
      781 gcacacggcc tctttgtttc ctgacacgga ctggaggtca gccgtgcgta acgagtggta
      841 taccgcgatc caggttccgc atctgaatgc ctggcgactc tcgctgacgc ttcacgcact
      901 catgcaggca gacgatctcg tctttctcgt taccggaagc gccaagcaga agattctgtg
      961 cgccatgtgt gcaccacaac ccgatctttc tcttccggcg gtgtatctcg cgactcgaca
     1021 tgcgtcggtc acgatgctca tcgatcgatc cgccgcgggt gccctacaac cgtaagtact
     1081 cgttgcatcg agaaaaagag gtgaattgga ggaagattca ggcaacaaaa aaaccgcgac
     1141 cttgtggtcg cggttttttt gatctgaacg gaatcaggga atctggagcg tcttgccgac
     1201 cttcagagcc ttcgggttgg tgatgttgtt ggcatcagca atcttcttcc agtttttggt
     1261 ggtgccatag accttcttgc tgatcttggc cagcgtatcg cccttctcga ccacgtaggt
     1321 gctcgccttg ccggcgcttt cttcgtgcac ttcgcccggg atggcattca catcgatcgg
     1381 ttcaggagtc ggttccggag caacttcggg ttcaggagcc ggttcaggag ccggttcggg
     1441 ttcaggagcc ggttctgggg tgggctcagg agccggagcg gtgtcaaccg ccggagcggg
     1501 ttctgcgacc ggttcggccg gtttttcttc cacgatgcgg aagttgcgca catcgatggc
     1561 gccgctgggg aacgtggcat cgggcttgcg cacgttgcca aaaatgcgca tcttggcacc
     1621 gcggtgctca gccagctgtt cagccttgcg accagtcagt tcgaggacct gaccgtcttc
     1681 ggtcttgaac atgatcttgc gaccgtcgat ggcatcaatg ccgcccgtca cggtcgtcga
     1741 ccgggacttc ttgctgaacc agtcggtaat cttcgagaag gtgcccttcg cgaaagacgc
     1801 ctgtgcgggc agaagcatag ccagcaccag aagagaaaga tacatttttc tcatcgccta
     1861 cattccccct ttgggatgct tgaaagtttc cggcccatca cgagccgctt gaatctataa
     1921 cgttcttttt cgttttgcaa gaggcgacgt gaaaaaagaa aaccgcccct cagggcggct
     1981 attcatacag gttttagccc tccaattcct tcggtcgaca caatttcttt tcttcggtcg
     2041 acacccgccc gcagccctgg cagaagtagc ggacctgtgc aaccatcggc ttgcagagat
     2101 gacgttcttc tccggctttt gcgccgcaga attcgcaggt gtagaccttt tttgtcggtg
     2161 tcggatcgca caggtgaccg tcttccgtcg ccacagcacc gcacgtcttg catttgcgca
     2221 gtgtattcat ctgtctttct cctcgaagca ttcgatatta agtgacatca aaacagtatc
     2281 ttttttccac ccattgcgca agcgaaggat tttcacaagc tccactctgt ccaattatta
     2341 cacattttga aaaatgccct caactttcta cctcgggcat gccgatagta gtacgggggc
     2401 agtgtttttt ttcacggatg aatccagctc aaggaggagc aacatggaaa aactatccgg
     2461 atccgttcat caacgcgcac tcgaatggtc cgcccgcttt cggtcagagt ctctgacact
     2521 ggtcgactac ctcgatcgcg gcaaggcacg ccagacgaga tccaccaatc tcgaacggga
     2581 cttcctggct ctctcgccct tcgggcagct gcagcaggtc tttgcgcaag ccaagcgcgc
     2641 tgagtcgacc accagttctc tgaacctcaa attcactctg ccgaccgctg atctggcaga
     2701 ccccgccaag ttcgccgagt gggaaaaaag tttcgatctc gttctccgca tgatggcgaa
     2761 ggatgacgcc gactatcaac ggctcaagga ccagttttcc gagatgttcg aaatcactcg
     2821 tgggcacttc actggtgccg agaactccct tgcgcagggt tctac
//
LOCUS       DAORHD010000579         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40815_length_2864_cov_3.867925, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000579 DAORHD010000000
VERSION     DAORHD010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40815_length_2864_cov_3.867925"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="amrB"
                     /locus_tag="PKO06_12460"
     CDS             complement(<1..609)
                     /gene="amrB"
                     /locus_tag="PKO06_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV70504.1"
                     /translation="MIRKPVVAGRFYPDDIDELIGVMENCAQSPLGVRAQVVPSHGRL
                     LGLIMPHAGYIFSGPVVTWGLKRLSAEQPHPTRLLLLGPKHTPYGAVAAVSGAQAWKT
                     PLGEVAVDDELRSALRESGEFEVDDGAHAYEHSLEVQLPFLQKQYGKTPFTITPVAMS
                     FTDYEDCRRWGTALAGVLTQPRFSSVCTIISSDFSHETPRDLA"
     gene            789..2033
                     /locus_tag="PKO06_12465"
     CDS             789..2033
                     /locus_tag="PKO06_12465"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNV70505.1"
                     /translation="MLRLRPLAPLLLILLLALVPALGQAEVKLGIDVLGEDHPELVRG
                     KKLALLVSGPSLDRQLDHVIDRLSRAATIEVIFTGEKYFRTTLPGASGTRRIDALTNA
                     PVHELLNPLKRPTAEQLGQAELILIDVQDVGIRYQQYVTLLAQFLELAREIKLPVILL
                     DRPNPINGTVVSGPVLEVNKRSRYGVYPIPLVYGMTIGELALYFNKAFGVGAALTVVG
                     MEGYSRRTLFRDTGLHWVPPSDHLPEPETPWVYAITGFLGEMGIFSTGVGTTRPFRYV
                     LAPWIDGELLAHKLSQRELPGVRFLPVMIQPYYGLYQNKRTPGIELVIDDPLGYDPVL
                     TGIAILQALWEIAPDRIPLKNPAVAAGLDTLLGSSKIREALLRGQPAREIQAGWQEDL
                     ARFMRERQAFLLYPETPLRIDE"
     gene            2030..2521
                     /locus_tag="PKO06_12470"
     CDS             2030..2521
                     /locus_tag="PKO06_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70506.1"
                     /translation="MNRMNRTGMSIVEVCIGVILLALILIPSMNVLSHGTATVTATRD
                     HLEAVYMAQRVMEAARTYDFNMLSKDQATRSGGDPNKTFEYDATFDDNTDPLDRVKQK
                     EQINGVVYEIDKASFKIEQVTNRSDTGAKVPVVAYRFLIVYKSKDGKNHELPVSAAIS
                     RRQ"
     gene            2523..>2864
                     /locus_tag="PKO06_12475"
     CDS             2523..>2864
                     /locus_tag="PKO06_12475"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70507.1"
                     /translation="MSQIRFGRGASLIEVMIALIITVILMTAVYQIFSQGRKGADEAM
                     RNHQINQDLQRVVDRLQNDIREASGIHPEFPPFYVPGVEKTTTPEDTKNIMVLQKAIV
                     DFKKNPATLSPG"
BASE COUNT          624 a          850 c          789 g          601 t
ORIGIN      
        1 tgccagatca cgcggggttt cgtgcgagaa atcactgctg atgatggtgc agaccgaaga
       61 aaagcggggc tgggtgagca cgccggccag ggcggtaccc caccgccggc aatcctcata
      121 gtccgtgaag gacatggcga ccggagtgat cgtgaacggc gttttgccat actgtttctg
      181 caaaaacggc agctgtactt ccagtgaatg ttcgtatgcg tgtgcgccgt catccacctc
      241 gaactctccc gattcacgca gagcgctgcg caactcgtca tcgacagcca cttcaccaag
      301 tggggttttc caggcctgcg ccccactgac cgcagccacg gctccatacg gggtgtgttt
      361 cggccccagg agcaacagac gcgtcggatg cggttgttcc gcactcagac gtttcagacc
      421 ccaggtaaca accggaccgc tgaaaatgta accagcatgc ggcatgatca gtccgagaag
      481 acgtccatgg gacggaacca cctgggcacg aactccaagc gggctctgcg cacagttttc
      541 catgaccccg atgagttcat cgatgtcatc gggatagaat cggccagcca ccacgggttt
      601 ccggatcatc gccacacctc cgtctgtttc agtctatcac acccgccggc ggaattcgtc
      661 ccatcctttg agaagtccgt gaaaacgttt catcgttctc gggcgtatgt actcgtgaga
      721 cacctgtgcg tctcggttgc tcttctctac ggaaaacggg taagatacga aggatgagga
      781 atttatcgat gctgcggctt cgaccactcg ccccgctgct gctgattctg ctgctggcgc
      841 tggtgccggc attgggacaa gccgaagtca agcttggtat cgacgttctg ggggaagacc
      901 accccgaact cgtacgcggc aagaaactcg ccctgctggt cagcggccct tctctcgacc
      961 gacaactcga tcacgtcata gatcgactgt cgcgtgccgc gacgatcgag gttattttca
     1021 ccggggagaa gtattttcgc accacgctgc ccggcgcttc aggcacccgg cgcatcgatg
     1081 cgctgactaa cgctccagtg catgagcttc tgaacccgct gaaacgcccg acagccgaac
     1141 aactcggtca ggccgagctg attctcatcg atgttcagga cgtcggcatt cggtatcagc
     1201 agtatgtcac gctgctggca cagtttcttg aactggcgcg cgaaatcaag ctgcccgtga
     1261 tactgcttga tcgtccgaat ccgatcaacg gaacagtcgt ctcggggccg gttctcgaag
     1321 tgaacaagcg cagtcgttac ggcgtgtatc cgattccgct ggtctatggc atgaccatcg
     1381 gcgaactggc tctctacttc aacaaagcgt tcggagtcgg tgccgcgttg accgtggtcg
     1441 gcatggaagg atactcgcgg cgcacgctgt ttcgcgacac cgggctgcac tgggtgcctc
     1501 cctctgatca tctgcccgaa cccgagaccc catgggtcta cgcgatcacc ggttttctgg
     1561 gagagatggg catcttttcc accggagtcg gcacgacgcg gccttttcgg tatgtcctgg
     1621 ctccctggat cgacggcgag ctcctggcgc ataaactgag ccaacgagaa ctgccgggtg
     1681 ttcgttttct cccggtcatg attcaaccat attacgggtt gtatcagaac aaacgcaccc
     1741 ccggaatcga gctggtcatc gatgatccgc ttggttacga cccggtactg accggcatcg
     1801 ccattctaca ggcactctgg gagatcgctc ccgatcggat tccgctcaag aaccccgccg
     1861 tcgcagccgg gctcgacacg ctgctcggca gctcgaagat acgcgaggcc ctgcttcgcg
     1921 gtcagccggc gcgagagatt caggccggct ggcaggaaga tctggcgcgc ttcatgcgcg
     1981 aacgccaagc ctttttactt taccctgaaa cgcctctgag gatagacgaa tgaaccgaat
     2041 gaatcgtacc ggcatgtcaa tcgtggaagt gtgcattggg gtgattctgc tcgccctgat
     2101 tctcatccca tccatgaacg ttctctctca tggcaccgca accgtcaccg caactcgcga
     2161 ccatctcgag gcggtctaca tggcacaacg agtcatggaa gcggctcgca catatgattt
     2221 caatatgctt tccaaagatc aggcgacgag aagcggaggc gatccgaaca agacttttga
     2281 gtacgatgcc acgttcgatg acaacaccga tccactcgat cgcgtgaaac agaaagagca
     2341 gatcaacggc gtggtctacg aaatcgataa agccagcttt aagatcgagc aggtcacgaa
     2401 tcgttctgac acgggggcta aggttccggt ggtggcttac cggtttttaa tcgtgtacaa
     2461 gtccaaagat ggcaaaaacc acgaactccc agtgtctgcg gctatctcac ggaggcaatg
     2521 acatgagcca aattcgattc ggccgcggcg ccagcctcat cgaggtgatg atcgccctga
     2581 tcatcaccgt gatcctcatg accgccgtct accagatatt ttctcagggt cgcaaagggg
     2641 ctgacgaggc gatgcgtaat caccagatca accaggatct ccagcgcgtg gtcgaccggt
     2701 tacagaatga tatccgtgag gcatccggca ttcatcctga gtttccgccg ttctatgttc
     2761 cgggggtcga gaagaccacc acaccggaag atacgaagaa tatcatggtc ctgcagaagg
     2821 ccatcgtcga cttcaagaaa aatccggcca cgctttcccc cggc
//
LOCUS       DAORHD010000580         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_40841_length_2863_cov_3.507835, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000580 DAORHD010000000
VERSION     DAORHD010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40841_length_2863_cov_3.507835"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..813)
                     /locus_tag="PKO06_12480"
     CDS             complement(34..813)
                     /locus_tag="PKO06_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70508.1"
                     /translation="MRNRAFAPLLIMLLFCLVHCLPTLAQREPPALSQIRENFAGAYQ
                     CLQERGLATFSVRVSSDQMRDICRTLKIDPPELWFDFRSPASFSLRPPSFDGVATAQR
                     QLIADLHDVIKNSIEGFFQAWEPVAYTNLIPTGSCSARLNGDNLILEYVEVEPHQTVT
                     LTFDRDFRLETMEARDAERKLASLVKMSWEKQGGRWILRTIQSEFRSQGTMTFMTRMA
                     IENIAVGSYYLPARVLIQALDGESGRPTQATFEIRLDEYRF"
     gene            944..2386
                     /locus_tag="PKO06_12485"
     CDS             944..2386
                     /locus_tag="PKO06_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70509.1"
                     /translation="MTTVLTLRRIARLAIPLAALALVALVTGCTGHGDLRFAPFPAEE
                     HYSVAGRINLTAVTESDLMWSLRGPAADIAVVTDFSKFVMSAGSASPKNAEKSGDFEL
                     ESVKPADDLMLQAKADKVVLLRHIYLRDLRETDSRKLDISLLTTAKALVWKKARERNI
                     ELTDADISAREYEPWLASITTALKFALQMPDKDVPKTILDLSMVTTPVTAAANVIIPR
                     EKLVREAYSVFENALLNESERMLEYLISPDFSNDWDTESSYTDFQKTLADYFTTYDIT
                     VASYTIQELEFLPGEIVRARVFAQITAMHRVTGLSHQTALYHTDTFWQREGTYWKIRR
                     NLPYKLTHPTQVAADARWGEIARAHVLLRDATYREDMEVFKTLFSTGFGNDWDIESTY
                     TDFLLQTKKQFDENDTQLASYTIRSIEFVTSELAKARISFHLKFISHATGYIEDWGLT
                     NAVVDWKWEDGAWKVFRNFPYKATHPKDLK"
BASE COUNT          657 a          860 c          730 g          616 t
ORIGIN      
        1 catacgagct cctgatgtga tggcacgcgg gcgttaaaaa cgatactcgt cgagtctgat
       61 ttcaaacgtc gcctgggttg gtcggcccga ttcaccatcg agcgcttgga tcaagacacg
      121 cgcgggcaga taatacgacc caaccgcgat gttttcaata gccattcggg tcatgaacgt
      181 catggttccc tgcgaccgaa actcgctctg aatcgtgcgc agaatccagc gcccaccctg
      241 tttctcccag ctcatcttca cgagactggc gagtttccgc tcggcatccc gcgcttccat
      301 cgtttccaga cgaaaatccc gatcaaacgt tagagtgacc gtctggtgcg gctcaacctc
      361 aacatactcc agaatcaggt tgtcgccgtt cagccgagcg ctgcacgaac cggtaggaat
      421 caaattggtg taggcgacag gctcccacgc ctggaagaag ccttcaatcg agttcttgat
      481 cacatcgtga aggtccgcga tgagctggcg ctgtgccgtc gccaccccat caaaactcgg
      541 ggggcgcaaa ctgaagctcg cggggctgcg gaaatcgaac cacaattccg gaggatcgat
      601 cttcagggtg cgacagatat cgcgcatctg gtcactgctc acgcgtacac tgaacgtcgc
      661 caggccacgt tcctgcaggc actggtaagc gccggcgaaa ttttcgcgaa tctgcgaaag
      721 agccggtggt tcacgttgcg ccagcgtggg cagacaatgg acgagacaga aaagcagcat
      781 gatcagcaac ggggcaaatg cacggtttcg catagtcatc ccctttcgtt gttccatcgc
      841 atactagacc tcccgctcct ctcctgtcaa agaaccggga cttcagaaaa cgacgctttc
      901 ttccgaaaga tgaggagatt ctgagatgag gaaaaggcat atcatgacaa cggtgctgac
      961 cctgcgaagg attgcccggc tcgccattcc gctggcggct ctcgccctgg tggctctggt
     1021 caccggttgc accgggcacg gcgatctacg tttcgccccc tttcccgctg aagaacacta
     1081 ttccgtcgcc gggcgcatca atctgaccgc ggtcactgag agcgacctca tgtggtctct
     1141 ccgcggtcca gccgctgaca ttgccgtcgt gaccgacttc tcgaagttcg tcatgtccgc
     1201 gggctctgca tccccgaaga atgccgaaaa atcgggcgac ttcgaactgg aatccgtgaa
     1261 acccgctgat gatctgatgt tacaggcaaa agcggacaag gtcgttctgc tgcggcacat
     1321 ctatctgcgc gatctgcgcg agacggactc ccgcaaactc gacatctctc tgctcacgac
     1381 ggccaaagcg ctcgtctgga aaaaggctcg tgagcgcaac atcgagctca ccgatgccga
     1441 catctcggca cgagaatatg agccctggct ggccagcatc accacggcgc tcaagttcgc
     1501 gctgcaaatg ccagacaagg atgttcccaa gacgattctc gatctgagca tggtcacaac
     1561 cccggtgaca gccgcggcaa acgtcattat tccgcgagag aaactggttc gcgaagccta
     1621 ttccgtattc gaaaacgcgc tgctcaacga aagcgagcgc atgctggagt atctcatctc
     1681 gcctgatttt tccaatgatt gggacacgga atcctcgtac acggactttc agaaaacgct
     1741 ggcggattac ttcaccacct acgacattac cgtggcgtcc tacacgattc aggaactcga
     1801 gtttctgccc ggagaaatcg ttcgcgctcg agtgttcgct cagatcacgg ccatgcatcg
     1861 ggtcaccggc ttatcgcacc agacggccct gtatcacacc gacacgttct ggcaacgcga
     1921 aggcacctac tggaaaatca gacgaaatct gccctacaag cttacccacc ccacgcaagt
     1981 cgctgccgat gctcgttggg gcgaaatcgc ccgagcgcat gttctgttgc gagacgccac
     2041 ctaccgcgaa gatatggaag tcttcaagac gctcttctcg accgggtttg gcaatgattg
     2101 ggatatcgaa agcacctata cggattttct gctgcagacg aaaaagcagt tcgacgaaaa
     2161 tgacacacaa ctcgcctcgt acaccattcg ctcgatcgag ttcgtgactt cggaactggc
     2221 caaggcccgc atctcgttcc atctgaagtt catcagccac gccaccggct acattgagga
     2281 ttggggcctg accaacgctg tcgtcgactg gaagtgggaa gacggtgcct ggaaggtctt
     2341 ccggaacttt ccctacaaag cgacccaccc gaaagatctg aagtaacgtg ccgttgctgc
     2401 ggcacacctg aaccgcgttt cacgtctcga cgtttcgcgg gaaactcacg gtcaaacctc
     2461 tgctcaacac atcattgagg ttagccagtt gtttcacact tcactgtcat tcgtcgcgct
     2521 gttgctgaga ccagctccct caggcttcgt ccagtcgaat atcagaggcc ttcttacgat
     2581 gcccgcttcg ggcgatttct ggcagcccct cttcaggcga ctgtatcctc agttttgcac
     2641 actgtcgtat tcttctcata cttagcaggc cgcaccatca aattcggcga cagtatatgc
     2701 atttggacaa tcacgcttcc agacaatcgc agcggcagct tgtttgttca ggctgaagcc
     2761 cagaaatact gcgtgttgct tacaacaaca tctggaaatt tcctacaaac aagaaccccc
     2821 cagactatcg tctgaggggt tcgagatctg cgttacgctt gct
//
LOCUS       DAORHD010000581         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41006_length_2855_cov_3.061786, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000581 DAORHD010000000
VERSION     DAORHD010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41006_length_2855_cov_3.061786"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKO06_12490"
     CDS             complement(<1..784)
                     /locus_tag="PKO06_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HNV70510.1"
                     /translation="MSETTDGPGNVTLHMTCGIDKLQSYDVSTPKGEFTVLHTPQFYF
                     GGAYGAPQLPIITKLVQIPFGADFKVVVKNSDTTEYDLSKYGITNRVYPRQPSAPKDG
                     SEVPFVYEKSAYIHKGFNGLPLTSMQEVGTMRNMRLALLTIAPIKYNAVDNKIAVYNN
                     IEFDVVMTGANLKSDKAAAEAYATPAFAWAENMVLVPNSLRFNVRNAAQSYLIITDPM
                     FKDAIAPFAAWKTQKGFKVDVVTTDKFGTGAAITEPLKAYIHN"
     gene            1217..1561
                     /locus_tag="PKO06_12495"
     CDS             1217..1561
                     /locus_tag="PKO06_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70511.1"
                     /translation="MSLLLSPLFLRHGVAALQDELARLADIAQGLATGDWPPGYDPND
                     AVLLQSLLPTLREATNAPLTIELLRSKPLQFFFHLFPDYLRRHDAALTSEDRCEIQRS
                     LDEYQRLTGIRP"
     gene            1558..2553
                     /locus_tag="PKO06_12500"
     CDS             1558..2553
                     /locus_tag="PKO06_12500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70512.1"
                     /translation="MTAEEGILRPPSESRSLLIRLVRNCPWNRCRFCPAYKAAAFSVR
                     PVDEVLADIEELAQDPLLQQCRNAFLQDADALVMPVESLVQILGVLRQRFPNLSRITS
                     YARATTLFHLRDDDLSRLHQAGLGRIHVGIETGCDEVLRFMDKGTSHNRQKEACIKVK
                     AAGIELCCYLMPGLGGRRWSVSHAQESARLVREVEPHHVRLRTCYVLAGTPLAEDLAA
                     GRYEPQTEEEIVREIRLLLTEIASVKTELISDHRINLLLELRGYLPDDYQRLLGIIDR
                     YLSLPTEDKQLFEAGRRLGLIRFLDELNHEETRAKIRAERHRYQPSLPVPVSVLF"
BASE COUNT          599 a          829 c          799 g          628 t
ORIGIN      
        1 gattgtggat gtaggccttc aggggttcgg tgatcgcggc accggtgccg aacttgtcgg
       61 tggtgacgac atcaaccttg aaacccttct gggtcttcca ggcggcgaac ggggcgatgg
      121 catccttgaa catcgggtca gtgatgatca ggtagctctg agcggcattg cgcacattga
      181 accgcagtga gttcggaacc agaaccatgt tttcggccca ggcaaaggcg ggggtggcat
      241 acgcttcagc agcggcttta tcgctcttca ggttggcacc ggtcatgacc acgtcaaact
      301 cgatgttgtt gtacaccgcg atcttgttgt caaccgcgtt gtacttgatc ggagcgatgg
      361 tcagcagagc gaggcgcatg ttgcgcatcg tgccgacttc ctgcatgctg gtcaacggca
      421 gaccgttgaa acccttgtga atgtacgcgg acttttcata cacgaaaggg acttcggagc
      481 cgtctttcgg agcggacggc tggcggggat acacgcgatt ggtgatacca tacttggaaa
      541 gatcgtattc ggtggtgtcg gaatttttca cgacgacctt gaaatcagca ccgaacggaa
      601 tctgaaccag cttggtgatg atcggcagct gaggagcgcc gtatgcgcca ccgaaataga
      661 actggggcgt gtgcagaaca gtgaattcac ccttgggggt ggaaacgtca taggactgaa
      721 gcttgtcgat accacaggtc atgtgcaggg tcacgttgcc cggtccgtca gtggtttcag
      781 acaccacctg aagaccgctg ctccgcgcgt cggacaacgt cacatccgcg gcgaacgccc
      841 cgctacaaac caacacaaca aggaccaata ctgaaaaaac tcgtttcact tcctgtcccc
      901 tttcttatgg tacttcatac ttgcttatat cgacccggtg cgggtttgca ccgaaccgct
      961 cataaccatt ctactcaagg gcctgccccg gggcctgagg ggctccactc agatcttctc
     1021 tgtgtgtaca cagacagaat agagggatgg ccgggcgaag tcaataggaa gagcccagta
     1081 ctctcaggaa aattcaaacc cgggaatctc ttgttctttt tccctgtctg tggcacagtt
     1141 gaagccgatc ctcgccgtgt ttccacttgc atattttgtg tatccggcca ccctatcgac
     1201 aggagcgatc actttgatga gcctgcttct ctctcccctc tttctccgcc atggtgttgc
     1261 agcgcttcag gacgaattgg cgcgacttgc cgacattgcc cagggtctgg caaccggaga
     1321 ctggccgccc ggctatgatc cgaatgacgc cgtccttctg caatcgcttc ttccaacgct
     1381 tcgcgaagcc acaaacgccc cgctcaccat cgaactgttg cgcagcaagc ctctccagtt
     1441 cttctttcac ctgttcccgg actatcttcg acgacacgat gcggcactga cctctgagga
     1501 ccgatgcgaa attcagcgct ccctggatga gtaccagcga ctgaccggca ttcgaccatg
     1561 acggctgaag agggaatcct ccgccccccg agcgagtcgc gcagcctcct gattcgcctg
     1621 gtgcgcaact gcccctggaa tcgctgtcgc ttctgcccgg cgtataaagc cgcggcgttt
     1681 tcggtgcggc cggtggacga ggtgctcgcc gacatcgaag aactggcaca ggatccgctt
     1741 ctgcaacagt gccgcaacgc ctttctgcag gatgccgatg ccctggtcat gcccgtcgag
     1801 tcgctggtgc agatccttgg ggttctgcga cagcgctttc cgaacctttc gcgcatcact
     1861 tcgtacgctc gcgcaacgac gctctttcac ctgcgcgatg acgacctgtc acgtctccac
     1921 caggcggggc ttggacgcat acacgtgggc atcgaaaccg gctgtgacga agtgctgcgg
     1981 ttcatggata aaggtacaag tcataaccgg cagaaagagg catgcataaa ggtgaaagcg
     2041 gccggcattg aactctgttg ttacctcatg cccggcctcg gggggcgtcg ctggtcagtg
     2101 tcgcacgcgc aggaatcggc ccgactcgtg cgagaggttg agccgcatca tgttcggttg
     2161 cgtacttgtt acgtgcttgc aggaacgcct ctggcagaag atctcgccgc aggaaggtat
     2221 gaaccccaga ccgaagaaga aatcgtgcgc gaaattcgcc tgctgctgac agagatcgcc
     2281 tcggtcaaga ctgagctcat cagtgaccat cgcatcaact tgcttctcga acttcgcggg
     2341 tatcttcctg atgattatca gcgccttctt ggaatcatcg acaggtactt gtccttgccg
     2401 acagaggaca agcaactgtt cgaggctggt cgacgactcg gactcattcg ctttctcgac
     2461 gagctgaatc acgaggaaac acgggcgaaa attcgagccg agcggcatcg gtatcaaccg
     2521 tctctcccgg tgcccgtcag tgttctgttc tgaggcaacg cggcccgaac ccggacgacc
     2581 acgagcagca tctcgcacct tcacacattc tcaccaactg ttgccggacg gctcagaagt
     2641 ttcgttcacg ccaagaggaa aactggacag aatcaaatgc ccctgggttt tcggcaccac
     2701 gggtggcgtg gtaggatact tgcatcatga gtgtgttccc gacagaggtt caggatgcgc
     2761 gcatcagcga tcacgccacg ctttccgatg ccacgacgac catacacgga gccgtcgtca
     2821 ttgaagccgg cggcgcgctc tgtcttgaga atgtg
//
LOCUS       DAORHD010000582         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41009_length_2855_cov_2.532857, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000582 DAORHD010000000
VERSION     DAORHD010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41009_length_2855_cov_2.532857"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1373)
                     /locus_tag="PKO06_12505"
     CDS             complement(24..1373)
                     /locus_tag="PKO06_12505"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNV70513.1"
                     /translation="MSPHTKGITKITKRNGQVVSFDPEKIANAIFRAARQVGGEDRRL
                     AEILAEKVVHHLEVTKKPPAIPTVEEVQDVIEKELIEAGHAKTAKAFILYRAEHTKLR
                     QGQEKIHATSGNIPYKKIWQVLNWSIDHDCCSIKRINRRIEDGTYSDLVQEVTEKYMA
                     DVRSAAEKVIARRDDVRIVIIAGPSSSGKTTTTIKMEEILKAHGLELVAMNLDNYFFD
                     LSHHPRDEFGDYDFETPEALDLKLINQHLSDLLKGKEIQMPIYNFKTGKREEERVPMK
                     IASNQIVLLDSLHGLYGPMTDSVPAAKKFKLYIETLCQIKLEDGQFIRWTDVRLLRRM
                     VRDSWHRSYNPRQTLEHWHYVRRSETKNIIPFLDTVDHIIDGSLPYELPVYKKHLFHH
                     FPSFMHDYKDDPKRQDAYIRAKRVHDMLQGFHTWEDESVIPKDALIREYIGGSIYKY"
     gene            complement(1480..2079)
                     /locus_tag="PKO06_12510"
     CDS             complement(1480..2079)
                     /locus_tag="PKO06_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70514.1"
                     /translation="METERRILLVEDEAILALQMKEAIRQMGYCVTGVCASGEKALES
                     VERQKPDLVLMDINIQGEMDGIETARMIRERYEIPSVFLTAHSDESTIARAKIVEPYG
                     YLLKPVNTKELQIAIDVALYKSRLDREKAQLTRDLQQALEKVKLLSGILPICCSCKKI
                     RNDQGYWEQIEVFIRDHSNAEFSHGICDECRRKLYEDVD"
     gene            complement(2082..>2855)
                     /locus_tag="PKO06_12515"
     CDS             complement(2082..>2855)
                     /locus_tag="PKO06_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNV70515.1"
                     /translation="TRFEPLELLIRCRDETIRTVMGSAAPLGGSFVGAHLIILYDITE
                     RKRGEQMEQSLREKEMLLKEIHHRVKNNLAVVSSLLSLQAKNKDAAVRALLEEGQQRV
                     KSMALVHEKLYQTKNVSAVNFDEYVRTIISEIMSLYQIDTRVIATEINIENIQLDLET
                     AIPCGLLINELLTNAFKYAFPDNRHGTLRIHFTQSQGIYSLLVQDDGIGLPSGFNPET
                     SSTLGLELVHVLAGQLGGELKLTSTGGVRAEVLFRRRRK"
BASE COUNT          616 a          807 c          707 g          725 t
ORIGIN      
        1 cacgggaaaa acttcgctcc tgatcagtat ttgtaaatac tcccgccgat gtattcccga
       61 atcagagcgt ctttggggat gacactctcg tcttcccagg tgtggaaacc ctgaagcatg
      121 tcatggacgc gcttagctcg gatgtaggca tcctgccgtt tcgggtcgtc tttgtaatca
      181 tgcatgaagg acgggaagtg atggaacaga tgcttcttgt agaccggaag ctcatagggc
      241 agcgagccgt cgatgatatg atcgacggta tccaggaacg gaatgatgtt cttggtctcg
      301 gaacgacgca catagtgcca gtgctccagc gtctggcgcg gattatagct gcgatgccac
      361 gaatcacgca ccatgcggcg cagcaggcga acatcggtcc agcgaatgaa ctgaccatct
      421 tcaagcttga tctgacacag ggtctcgata tacagcttga acttcttcgc cgccggcaca
      481 ctgtcggtca tggggccata caagccatgc agagaatcaa gcaaaacaat ttgattcgag
      541 gcgatcttca tgggcacacg ctcttcttcg cgcttccccg tcttgaagtt gtagatcggc
      601 atctgaatct ctttgccctt cagcaggtcg gaaagatgct gattgatcag cttgagatca
      661 agcgcttcag gggtctcgaa atcgtagtcg ccgaattcat cgcgcggatg atgcgacaga
      721 tcgaagaagt agttgtcgag attcatggcg accagttcga gaccgtgcgc cttcaaaatc
      781 tcttccattt tgatcgtggt cgtcgtcttg cctgatgagg acggaccggc aatgatcacg
      841 atgcgcacgt cgtcgcggcg ggcaatcacc ttttccgcgg ccgagcgcac gtctgccatg
      901 tacttttcgg tgacttcctg caccaggtcc gaatacgtgc catcttcaat acgccgattg
      961 atgcgcttga tactgcagca atcatgatca atcgaccagt tcaacacctg ccagattttt
     1021 ttgtacggga tgttgccgga ggtggcgtgg atcttttcct gcccctgccg caacttcgtg
     1081 tgttcagccc gatagagaat gaaagccttg gccgttttag cgtggccagc ttcgatcagt
     1141 tccttctcaa tgacatcctg aacctcttcg acggtcggaa tcgccggcgg ttttttcgtc
     1201 acttcgaggt gatgcacgac cttctcggcc agaatctcgg ccagacgacg atcttcacca
     1261 ccaacctgac gcgcggcacg aaagatggca ttcgcgatct tctccggatc aaagctcacc
     1321 acctggccgt tgcgcttggt gattttggtg attcccttcg tgtgtgggct cattccttat
     1381 gtgtctcctt ctctgaaatg gaacaatcgg tacagtaccg tttcgactcg gcgcttgtca
     1441 aacgtcttgc acgtttcaat accgtttcgc gtcatcccct caatcgacat cttcgtacaa
     1501 cttccggcgg cactcgtcac agatcccgtg actgaattcg gcgttggaat gatcacggat
     1561 gaacacctcg atctgctccc agtatccctg atcattacga attttcttac acgaacagca
     1621 gataggaagg atgccgctca gcagcttcac cttttccaga gcctgctgca gatctcgggt
     1681 caactgagcc ttttcccgat ccagtcgcga cttgtacaaa gcgacgtcga tcgcaatctg
     1741 cagttctttc gtattgaccg gcttgagcag gtacccatag ggttcgacga tcttggcccg
     1801 ggcaatggtc gattcatccg aatgcgccgt cagaaagaca ctcggaattt catatcgttc
     1861 gcgaatcatt ctggccgtct cgatgccatc catttcgccc tgaatgttga tatccatcag
     1921 caccagatct ggtttctgcc gttcgacact ctcgagggct ttctccccgg atgcgcagac
     1981 tccggtaacg caatacccca tctgcctgat ggcttctttc atctgcaatg ccagaatcgc
     2041 ctcatcctcg accagcagaa tccgtctttc tgtttccatg cctattttct ccttcgtcga
     2101 aacagcactt cagcgcgcac cccgcctgtc gaggtcagct tcaactcgcc cccgagttgt
     2161 ccggcgagca cgtgaaccag ctccaggccc agagtcgaag acgtttccgg gttgaaccct
     2221 gaaggcagac cgatgccgtc atcctgaacc agcaacgaat agataccctg tgactgcgtg
     2281 aaatggatcc tcagagttcc gtggcgattg tcgggaaacg catatttgaa cgcgttggtc
     2341 agcagttcgt tgatcagcag accacaggga atggcggttt cgagatcgag ttgaatgttt
     2401 tcgatgttga tctcggtcgc aatgactctg gtatcgatct ggtacaagct cataatctcg
     2461 gaaatgatgg ttctgacgta ttcgtcaaaa ttgaccgccg agacattttt cgtctggtac
     2521 aacttttcat gcaccagagc catcgacttg acccgttgct gaccctcctc gagaagcgct
     2581 cgaaccgccg catccttgtt cttcgcctgc agtgacaaca gactcgagac aaccgccagg
     2641 ttgttcttga cgcgatgatg aatctctttc agcagcattt ccttttcacg cagcgactgc
     2701 tccatctgct cgccccgttt tcgttcggtg atgtcataga gaatgatcag atgtgcgccg
     2761 acaaacgatc cacccagcgg agccgcgctt cccatcacgg ttcgaatggt ctcatcacgg
     2821 caacgaatca gtaactcaag tggctcgaat cgcgt
//
LOCUS       DAORHD010000583         2853 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41047_length_2853_cov_3.669049, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000583 DAORHD010000000
VERSION     DAORHD010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41047_length_2853_cov_3.669049"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PKO06_12520"
     CDS             <1..903
                     /locus_tag="PKO06_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNV70516.1"
                     /translation="ASARAVVVPGGGVLQSATSAFSLLYYVGLLMFGRAFGARLLMPA
                     QGFGPWAPVNPTFWPELFAPLLAVVARLLCEYADHLSVRDTAAARAFAGLAFPALSMP
                     VTADLVFALQPHRQRLMSNINLDDPTRPLRLGVVLRSQHPAATQLLTLLQGSLLSGRP
                     LQIIPLSLRPGEDEEIWRAAPHLQAPLNLLPQQPFHEVLTTLDLLLSMRLHACIIAAQ
                     MRLPFIGIAVDPKIAQFAVDAGMPCLDASVPLTAEPLIGAFQSLATLPDPGAPLAALV
                     ATRRDQALATLETCARLLPPSPLS"
     gene            1067..1549
                     /locus_tag="PKO06_12525"
     CDS             1067..1549
                     /locus_tag="PKO06_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70517.1"
                     /translation="MKRVFKMFLFVALFAAVAMAAQAGSYFKQGHSLYLDGKVHIRFD
                     HLLLAWSHDGKVDRIRYELTCLAGKAVLDKVEYLDKAVEGKEISRWTESEQPLNLDFS
                     AKFSHLDKHHRVQISKTNFPAITPFDLNTAKVCFHIKASDRNYKVFWLPKTGEVYAIW
                     "
     gene            complement(1585..>2853)
                     /locus_tag="PKO06_12530"
     CDS             complement(1585..>2853)
                     /locus_tag="PKO06_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HNV70518.1"
                     /translation="AFPRHDQSRQVLEVITRHPGEGGIVYAQTRREVERLAKTLAKSG
                     VLCAPYHAGLAANVRERVQDDFVHERLDVVVATIAFGMGIDRSNVRYVVHANTPRSIE
                     HYQQESGRAGRDGEPAECILLFSAGDLATHRALSLRDDSVTAERRRVIERQLKEIGRY
                     AVAPVCRHWLLAEHFGQPYPDPTAERQSPGPDSGCGACDVCLGETQALPDDEALLTAQ
                     KIISAVFRTESRFGAGYVIKVLLGADDERILANRHQDLRVFGLMKESSEAILRGWIDQ
                     LVIQGFLAVAEGEYPLLQMTRAGLELCKGQGTVRLGRPVPILKKRKGRRSAAGPAQAT
                     DSMATGMFERLRQLRLLLARAKGVPPYVIFPDSVLISFAALRPATLPEVREIKGVGDS
                     KLARYGPVFLQVLGGASPEEAAAAWAKENG"
BASE COUNT          544 a          948 c          780 g          581 t
ORIGIN      
        1 gcctccgcac gcgcggtcgt tgttcccggt ggcggcgtgc tacagtcagc gaccagcgcc
       61 ttcagcctgc tctactacgt gggtctgctg atgttcggtc gcgccttcgg agcgcgtctg
      121 cttatgccgg cacagggttt cggtccctgg gctccggtga acccgacctt ctggcccgag
      181 ctgttcgccc ctctgttggc cgttgtggcg cgcctcctct gtgagtatgc cgatcatctc
      241 tcagtgcgcg ataccgctgc cgcgcgcgcg tttgcgggtc tggcattccc ggcactttcc
      301 atgcccgtga ccgctgatct cgtctttgcc cttcagcctc atcggcagcg actcatgtcg
      361 aacatcaacc tggacgaccc gacccgcccg cttcgtctcg gcgtcgtcct gcgcagccag
      421 catcccgccg cgacgcaact gctgaccctg ctgcagggat cgctgctttc cggccgaccg
      481 ctgcagatca taccgctgag cctgcgcccc ggtgaagatg aggaaatctg gcgcgccgca
      541 ccccacctgc aggcccctct caatcttctg ccgcaacaac cattccacga agttctgacg
      601 acgctcgacc tgctgctttc catgcgactt catgcctgca tcatcgccgc gcagatgcgg
      661 ttgccgttta tcggtatcgc ggtcgatccg aaaattgccc agttcgcagt cgatgccggg
      721 atgccctgcc ttgatgccag tgttcccctg accgcagagc cgctgatcgg tgcattccag
      781 agtctggcaa ccctgcccga tcctggcgcc ccactcgccg cactggtcgc aacccgtcgt
      841 gaccaggctc tcgccacgct cgagacctgc gcacgcctgc tgccgccctc ccctctttcc
      901 tgagcaggct cccggcaacg ggctccgacc gactcggtat cctcgcatat aatgcgacaa
      961 taacagtagc atattttcct tgcacttcca gccaaagaga ggtaaagtga cgcaaagcgg
     1021 aggtttcttc cgctgattta gatacatcac tcagaggagg ctctccatga aacgtgtttt
     1081 caaaatgttc ctgttcgtcg ctttgtttgc ggcagtggcc atggctgctc aggcgggaag
     1141 ttacttcaag caggggcaca gcctgtatct cgacgggaaa gtgcacatcc gttttgatca
     1201 cctgttgctg gcctggtcgc atgacggcaa ggtcgatcgc attcgctatg aactgacctg
     1261 tctggctggc aaagcggttc tcgacaaggt cgagtatctc gataaagccg tcgaaggcaa
     1321 ggaaatttct cgctggaccg agtcagagca gccgctcaac ctggactttt ccgcgaagtt
     1381 cagccatctc gacaagcatc atcgtgtcca gatctcgaag acaaacttcc ccgcgatcac
     1441 cccgttcgac ctgaacaccg ccaaggtctg tttccacatc aaggcctcgg atcgcaacta
     1501 caaagtgttc tggctgccga agaccggtga agtgtacgcg atctggtaat cgattcgttc
     1561 tccagaacgg gtgagccaaa cggctcaccc gttttctttt gcccaggcag cggcagcttc
     1621 ttcgggcgat gcgccaccaa gcacctgaag aaacaccggc ccgtagcgcg ccagcttgct
     1681 atcgccgaca cccttgatct cgcgcacctc aggcagagtc gcgggacgca gcgcggcaaa
     1741 cgagatcaat accgagtcgg gaaagatcac atagggcggc acacctttcg cgcgagccag
     1801 cagcagacgc agttgccgca atcgttcgaa cataccggtc gccatcgagt cggtggcttg
     1861 cgcaggtccc gccgctgatc ggcgcccctt ccgctttttc aggatcggca ccggtcgtcc
     1921 cagtcgcacg gttccctgac ctttgcagag ctcgagcccc gcacgcgtca tctgcaacag
     1981 cgggtattct ccctctgcca cggccagaaa gccctgaatg accagctgat caatccaacc
     2041 gcgcagaatg gcttcagaag attctttcat caggccgaac actcgcaggt cctggtgacg
     2101 attggccaga attcgctcgt catcggctcc cagcaacacc ttgatgacat agccggcgcc
     2161 aaagcggctt tccgtacgaa atacggcact gatgatcttc tgcgcagtca acaacgcctc
     2221 atcatcggga agtgcctgcg tttctccaag acagacatcg caggctccac atcccgaatc
     2281 aggacctggc gactgccgct ctgccgtcgg gtctggatac ggctggccga agtgctcggc
     2341 cagaagccag tgccggcaca ccggcgcgac cgcataacgc ccgatctcct tcagctgtcg
     2401 ctcgatcacc cggcggcgct cggcggtgac ggaatcgtca cgcagactga gcgcgcgatg
     2461 tgtcgccagg tctcccgcgg aaaaaagcag aatacactcg gcgggctctc catcacgacc
     2521 cgcgcgaccg gattcctgtt gatagtgctc gatagagcgg ggggtgttcg cgtgaacgac
     2581 atatcgcaca ttcgaccgat cgatacccat gccaaaggca atggtggcaa ccacgacgtc
     2641 aagacgctca tgcacgaagt catcctggac tctttcacgc acgttggccg cgagacccgc
     2701 gtgatagggc gcgcagagaa caccggactt cgccagagtc ttcgcgaggc gttcgacctc
     2761 gcggcgcgtc tgagcataga cgatgccgcc ttctccgggg tgacgcgtga tcacctcgag
     2821 tacctgccgc gactgatcat gtctcgggaa agc
//
LOCUS       DAORHD010000584         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41075_length_2852_cov_3.602074, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000584 DAORHD010000000
VERSION     DAORHD010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41075_length_2852_cov_3.602074"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKO06_12535"
     CDS             complement(<1..689)
                     /locus_tag="PKO06_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70519.1"
                     /translation="MKQLVRLATVLLFVAGALVVCAEPVVTEPASVASTSEILKQGNP
                     EDIASMDAVTTSPETTASAASGAIASSPVVNAGPEANASDAILVLIQKKLPAGWKMLI
                     ASDTLTFERAGDIWVMNVNRINAEPKLEFPKERQAIIKSQGKKTQARMVFKMTPRLTL
                     EQMAVLDTENAKIDVEIASLPEKLGISGFLDVELSRKVGPVYTATAPEDVKRVEEYQK
                     LREELSSKRLK"
     gene            complement(974..2053)
                     /locus_tag="PKO06_12540"
     CDS             complement(974..2053)
                     /locus_tag="PKO06_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70520.1"
                     /translation="MQLKWTQFFWRQLLRLRRKAHLLDYFFWEATLRCNLACRHCGSD
                     CLKDPRTSDLSADRVIGVFRNIAERYFPSLVMVAVMGGEPLVRPDLFEILAEVQRFGF
                     PWGLVTNGMLVDEHIVQKCQSTGMKTVSVSIDGTSTFHNAFRGHPESYERAEQAVRLF
                     VASRAFQVVDVITCANAENLDHLENLGHHLHALGVQSWRLFTIFPKGRATTAPHLIAS
                     SSILRRTFSLIEQMRNIYPGWAVSYSEEGYLGPAWELKVRDIPHYCGAGINIGGLLAD
                     GSYSACPSLSREWIQGHVDELPFMEAWDRRYRNMRSRDWMKNDSCGECAEWKNCQGSS
                     LHLWDWKRHCPAVCHLKLLNEPGSC"
BASE COUNT          606 a          782 c          825 g          639 t
ORIGIN      
        1 tatttcaggc gtttcgagga cagttcctcg cgtaacttct ggtactcttc aacgcgcttc
       61 acatcctcgg gcgcggtggc ggtgtacacc ggtccgacct tgcgcgacaa ctcgacatcg
      121 agaaatccgg aaatgcccag tttctctgga agagaggcga tctcgacatc gatttttgcg
      181 ttctcggtat cgagaacggc catctgttcg agcgtcagac gcggagtcat cttgaacacc
      241 atacgcgcct gtgtcttctt cccctgtgac ttgatgatgg cctgtcgctc tttcggaaac
      301 tccagcttgg gttcggcatt gatgcggttc acgttcatta cccagatgtc gcccgcacgt
      361 tcaaaggtca gcgtgtcgct ggcaatcagc atcttccagc ctgcggggag ttttttctga
      421 atgagcacca gaatcgcatc actggcgttc gcttcaggcc cagcgttgac caccgggctg
      481 gaagcaatcg cacctgaggc ggcgctggca gtcgtttcgg gcgacgtggt gacagcgtcc
      541 atcgatgcga tatcttcagg gttgccctgt ttcagaatct ctgacgtcga agccactgac
      601 gccggttcag tcaccaccgg ctcagcgcac acaaccaggg ctccggcaac gaaaagcaga
      661 acagtggcca atctgacgag ttgtttcatg tacatttcct gtgcatcaat ttttggtagc
      721 tccattgtat cagaagtccc gtgccaccgt tcaacaccac ttcttgatgt aatgtagacg
      781 gagacgactc gtgcgtgtct cgtggcctga gactcttcgt cttcggacgg catcgcgaca
      841 acggctgcac tcgcaggcat accaaatcga cttgcgtcag caggaatttt cgcattctag
      901 acctggaggt cggggacttc ccctggagga agagtcccga aacaaccacc gggtgtggtc
      961 tttgatttcg atgtcagcaa ctgcctggct cgttcagcag tttcaggtga cagacggccg
     1021 ggcagtgtcg tttccagtcc cagaggtgga gactcgagcc ttggcagttt ttccattctg
     1081 cgcattcgcc gcaggaatcg ttcttcatcc agtctctcga tcgcatgttg cgatagcgac
     1141 ggtcccaggc ttccatgaat ggcagttcgt cgacatgacc ctgaatccat tcccgggaaa
     1201 gcgacgggca ggccgagtaa gagccgtcag ccagcagacc gccgatgttg atccctgctc
     1261 cgcaatagtg aggaatatcg cgcaccttca gctcccaggc gggtccgaga tagccttcct
     1321 cggaataact taccgcccag cccggataga tgttccgcat ctgttcgatc agggaaaacg
     1381 ttcgccggag gattgaggaa ctcgcgatga gatgaggcgc cgtcgtggct cgtcctttgg
     1441 ggaagatcgt gaagaggcgc caggactgca caccaagtgc atgcagatgg tggcccaggt
     1501 tctcaagatg atcgagattc tcggcattgg cgcaggtgat gacgtcaacc acctgaaagg
     1561 ctcgcgaagc gacaaagagg cgcacggcct gctcagcccg ctcatatgat tcggggtgcc
     1621 cacgaaatgc gttgtgaaat gtcgaggtgc catcgatgct gacagagacc gttttcatgc
     1681 cggtcgactg gcacttttgc acgatgtgtt catctaccag catcccgttg gtgacgagac
     1741 cccaggggaa gccaaaccgt tgtacttccg cgagaatctc gaacaggtca ggtcgcacca
     1801 gcggttcacc acccatgacc gcgaccatga ccagcgacgg aaaataccgt tccgcgatgt
     1861 tgcgaaaaac gccgatcact ctgtctgcgc tcaagtcgga cgtgcgcgga tccttgagac
     1921 aatcactgcc gcagtgccga caggcgagat tgcagcgcag ggtggcttcc cagaaaaagt
     1981 aatccagcag atgtgccttg cggcgcaatc tcaagagctg tcgccagaag aactgggtcc
     2041 acttgagctg catacagcag tgggctacct gggtccgctt gctggaggcg ttgctggagc
     2101 cgtcgatgac gccggaggtg atggtacggg cggcggcgga gaacccgcgg agatcgccgt
     2161 gggagggccg tagatcagct tgctggaact cggctgaaag atcgatgaga tcgatccgta
     2221 gacagcggag gtaaagcttg caataccgag tacgagcgcg agaagcgaga gaacaaagta
     2281 gagaatcgct ctgacaaccc ttccaaaacg gttcattggt ctgcctcccg ctagtcgccg
     2341 gctacgatga tttcgcccgt cttcttgttg atgatgaccc gaaaggagat cggatcctcg
     2401 gtacgatcat tggtgaaatg ttgcgtgtat cctgcccagc agaaggcagg tcaccagaat
     2461 caggaatgat tttgcagtca tcagacgatg aactcctcgc tcttcgatct cattgcctca
     2521 cccggtgtta ttctgcattc tcccggagag acagtttctc atgaaagtcg gtcgggcgct
     2581 aacttatttt tccgatcctg gcacaatttc attgtccggc aggatcctcg gcagtatact
     2641 ttccgtatcg ggctgatagg atgcagaggg aagccagatg ggaggatgcg tgggaggaga
     2701 ctgccaggct gaaacaccgc tgagggtcag aactgtcatt gtcgctgcga ttgtcatcag
     2761 cgtcgtggtg cgtttcgcgg acagccagct tctggaaggc atggagctgc tgcgactggt
     2821 tgcgattgcc ggttccgcga tccttctggc gg
//
LOCUS       DAORHD010000585         2850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41137_length_2850_cov_2.174240, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000585 DAORHD010000000
VERSION     DAORHD010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41137_length_2850_cov_2.174240"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKO06_12545"
     CDS             <1..312
                     /locus_tag="PKO06_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     II"
                     /protein_id="HNV70521.1"
                     /translation="DPAETRAIKELLQERAVRIPINSTKSMTGHLIAAAAAVEAVVCV
                     QSIRHGKVHPTRGLLRPDPLCDLDYVAGKARDVRIRHALSNSLGFAGINSTLVFSRLD
                     P"
     gene            309..1193
                     /locus_tag="PKO06_12550"
     CDS             309..1193
                     /locus_tag="PKO06_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70522.1"
                     /translation="MMNQGLSPFCDVRGWVVTTDPQVAAWGAERGLTLRQLAKEELAT
                     GLALNPKEIRRFDRQSLLLLHVLHLAGVPGWPEPMRHQVPVQLAVGPAQTDLPALHRW
                     GARVEPAEALPMVQPAQAIGLLPNTPLSHLSIACGLRGEASVWAGFADAGCRAVAAAS
                     LYLCDQSREQVLILAVSSPDNYFVRQVFERGFALQPEQMSEVAVALMLTKSSSNLCLS
                     HIRQHLSSAAAATKGLPGVPAHSIQTSGGLRFSIPDCLTATPLLGLSALAAQAEPASL
                     IISTPEGFEWQFDLGGAS"
     gene            1190..2398
                     /locus_tag="PKO06_12555"
     CDS             1190..2398
                     /locus_tag="PKO06_12555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HNV70523.1"
                     /translation="MSRARVVMTGVGVICACGHDGDSFWNALSRGESGLRAIRRFDTS
                     TLVTKVGGEVDLTSLDKPPCTVRSPIEDTKAYLAWNALQQILHLVQKEDALITTVGLE
                     RIDLATIVGADTETATSPVCPETPPVVLPARLWGAAGVRGPVATQVAACAAGTLAIGH
                     AFRLIRSGWATGVVAGGVDSLLFPFGIHAFNSIGALSEQNERGAAALAPFDRFRSGTL
                     LGEGAGYVMLEELDRAIRLGHTPLAEILGFGGSMDAYHPVMPCPNGLGASLAMRRALD
                     DARLAPERIQYINAHGTGTWHNDVMEAKAILQVFGDRGRRVPVSSTKPFFGHLLTAAG
                     AVECISALLPLTRGLLPPTLHWRNPDPDIPLDCIPETARPDSEIDVVMSNSYGLGGQN
                     ASLVFGRWHP"
     gene            2395..>2850
                     /locus_tag="PKO06_12560"
     CDS             2395..>2850
                     /locus_tag="PKO06_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNV70524.1"
                     /translation="MTTRDSHEVLVVTGTGLITPYGQGFEPVRQGIEEGRLAWGELTA
                     FDCTSWPVRHGGWVQDPTPDQLGTFNNKLRNMGKYVRLGVIAARQALQAADVVAGKIP
                     PERLGAFICTGTHGHNAEGLFPAFAEAAGENGALDVRRLGESGIDRVHPW"
BASE COUNT          492 a          815 c          942 g          601 t
ORIGIN      
        1 gatcccgccg aaacgcgggc gatcaaagaa ctgctgcagg aacgggcggt gcgcatcccg
       61 atcaattcca ccaaatccat gacagggcat ctgattgcgg cggcggcggc ggtggaagcg
      121 gtcgtctgtg tccagtccat tcgacatgga aaagtgcacc cgacgcgtgg tttgctgcgc
      181 cctgatccgt tgtgcgatct ggactacgtt gccgggaagg cacgcgacgt ccgcattcga
      241 catgcgctct cgaattctct ggggttcgcc ggcatcaaca gcaccctggt cttctcgcgt
      301 ctcgatccat gatgaatcag ggtctttcgc cgttctgtga cgtgcgcggc tgggtcgtca
      361 ccacggatcc gcaggttgcg gcctggggag ccgagcgtgg cctcacgctc cggcagctgg
      421 cgaaagagga attggcgacc gggctggcgt tgaatcccaa ggaaatacgt cgtttcgacc
      481 ggcagagcct gctgttgctg cacgtgttac acctcgcggg cgtgcccgga tggcctgagc
      541 cgatgcggca tcaagtgccg gtgcaattgg cggtcggccc cgcacagacg gatctgccgg
      601 cgctgcatcg ctggggtgcc cgggtcgagc cggccgaagc gctcccgatg gttcaaccgg
      661 cgcaggccat cgggctgctc ccgaacacgc ctctttctca tctctcgatt gcgtgtgggt
      721 tacggggtga agcctctgtc tgggcgggtt tcgccgatgc cgggtgtcgg gccgtggcgg
      781 cggcttctct gtatctctgc gaccaatcgc gcgaacaggt gctcattctc gccgtaagca
      841 gcccggacaa ttatttcgtg cgccaggtgt tcgaacgtgg cttcgccttg caaccagaac
      901 aaatgtccga ggtggcggtc gcgctcatgc tgactaaaag ctcgtctaat ctctgtctgt
      961 cacacattcg acaacacttg tcatccgcag ccgctgcaac gaaaggcctg cccggtgtcc
     1021 ccgctcactc gatacagacc agcggggggc ttcgattttc gattcccgac tgtctgaccg
     1081 cgacccccct gcttggcttg tcggctctgg cagcgcaggc agaaccggca tcgctgatca
     1141 tctcgactcc agaaggtttc gagtggcagt tcgatctcgg gggcgcgtca tgagccgtgc
     1201 gcgcgtggtg atgaccgggg tcggggtcat ctgtgcctgc gggcatgacg gtgattcgtt
     1261 ctggaacgcg ctctcacgtg gcgagtccgg gctgcgcgcg attcgtcgct ttgatacctc
     1321 gaccctggtg acgaaggtcg ggggagaggt cgatctgaca agtctcgaca agcctccgtg
     1381 taccgttcga agtccgatag aagataccaa ggcatatctg gcctggaatg ccctgcaaca
     1441 gattcttcat ctggtacaaa aggaagatgc cctgatcacg acggtcgggc tggaacgaat
     1501 cgatctggcc accattgtgg gggcggatac ggaaaccgca acctctcccg tctgccccga
     1561 gacgccaccg gttgtccttc cggctcgtct gtggggtgcc gcgggagtgc gggggccggt
     1621 ggcgacccag gtcgcggcct gcgcagccgg tacgttggcg atcggtcacg cgtttcgtct
     1681 gattcgcagt ggttgggcga cgggagtcgt cgctggaggt gtcgattcgt tgttgtttcc
     1741 gtttggcatt cacgccttca attcgattgg ggccctgtct gaacagaacg aacgtggagc
     1801 ggcagctctc gcaccgttcg accggttccg ttccggcacc ctgcttgggg agggcgctgg
     1861 gtatgtgatg ctcgaggaac tcgatcgggc cattcgactt ggacatacgc cgctggcgga
     1921 gatcctgggt tttggcggtt cgatggatgc ctatcatccg gtcatgccgt gcccgaacgg
     1981 actcggggcc agtctggcga tgcgtcgcgc gctcgatgac gcgagactcg ctccggaacg
     2041 gattcagtac atcaatgctc acggcacggg aacgtggcat aatgatgtca tggaagcaaa
     2101 agccattctg caggtgtttg gggatcgagg gcgccgggtt ccggtcagtt cgaccaagcc
     2161 gtttttcgga catctgctca cggcggcagg ggcggtcgag tgcatttcgg cgcttcttcc
     2221 cttgacgcgc gggttgctac cgccaacgct tcattggcgc aaccctgacc cggacattcc
     2281 gcttgactgt attcctgaga cggctcgtcc tgattctgag atcgacgtgg tgatgagcaa
     2341 ctcgtacgga ttgggtgggc agaacgcatc gctcgtgttc ggcaggtggc atccatgacc
     2401 acaagagatt cgcatgaagt cctggtcgta acgggtacgg ggttgatcac gccctacggg
     2461 caggggtttg aacccgtgcg ccagggaatc gaagagggaa gattggcctg gggagagttg
     2521 acagcgttcg attgtaccag ctggccggtt cgacatggcg gatgggttca ggacccgaca
     2581 cctgaccaat tgggaacctt taacaacaag ctgcgaaaca tgggaaagta tgtgcggctc
     2641 ggggtcatcg cggcgcgtca ggcgctgcag gctgccgatg tcgtcgcggg gaagattccc
     2701 cctgaacgtc tgggggcctt tatctgcacg ggaacgcatg ggcataacgc ggaagggttg
     2761 ttccccgcgt ttgccgaagc ggcgggagag aacggagccc ttgatgtgcg tcgcctggga
     2821 gagtcaggga tcgatcgcgt tcacccctgg
//
LOCUS       DAORHD010000586         2849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41146_length_2849_cov_3.970651, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000586 DAORHD010000000
VERSION     DAORHD010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41146_length_2849_cov_3.970651"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2780)
                     /locus_tag="PKO06_12565"
     CDS             complement(<1..2780)
                     /locus_tag="PKO06_12565"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70525.1"
                     /translation="MLEQIDLFSSLSTKELRDIEQIALTRSVLRGEVIFSQGDFARDL
                     FLVQNGQVEILLKDYKQDLKQVAVMRNGDFFGEMALFDKDSVRSATARALQNSTLIII
                     PGTGFERLLQEKPSISFKLLNALSKRLKDTSQKAVAGSSSIVTAGTPTEARIITVASP
                     RNGTGKSIFATTLAQILAHETSRRVLLIDLDFSFADSTYLLGIVSPKSVIEMCQLVEK
                     GEQAWETLSRGLVRHAESFFSLPAPNNIVDGEKIRPGILISMVKALRKFFDYIILDTD
                     SPINENLLNAIDLADRVIFLVNLETVRMIKGATRYFQGLGKLDYNEDRFCLFAHRGGS
                     EFQPERYRSLFRFPLYGALANLDCTGIDYGKTAYQLQPNCAYCHSLRQIIRSLLKEQV
                     RAPQTETNKGFFFRLFYDESKPQTNDASASEQLLESQGGKKQTSPKILESDLSALLKY
                     IRTNVLYGNLEEAREQTQQLLTYCNWSASIFQILGEIHYGEHNFSQAIDVLKQAIHLD
                     PENYMAMGFLAEITNDDALFKKAVNLVESQIGEKTRYPDLYNDLGRLWELHRDWQKAV
                     DNYHKALALNPQYLEAKMHLATVLGKQELFHEAIQVLEEIHPKNIRVFYMLGDYYYSV
                     GQFAESKVNFLSAEEINPNYYDLTTRLKHLEDYFDRLVSLIEMHQKIIKDHPNYPDIH
                     LKLGNFYTLLGQRDEAVEEFRRALAINPNYQEAKTQLETWMKGDNLLPHTTSLPATAA
                     PTGFALSLTFPCSLGKENPTGCPTNCTLTVRNLRTSRSAEIDMPCKHNKGETATTVDI
                     SALGPISRGDILLVKIKNRTTGKSSLALPHPVSEDEVKSRDVVISLENGALDETEGPQ
                     TPIRYFFVRTRNAVLAQTLSRAPQSTKIVLENPRLPLRAVGQLNADDPTEVTFVLKSP
                     RGREVVQVGDTLR"
BASE COUNT          605 a          720 c          845 g          679 t
ORIGIN      
        1 cgcagagtat cgccgacttg aaccacttct cgaccgcgcg gagacttcag gacaaacgtc
       61 acctccgtcg ggtcatccgc attcaactgg ccgaccgcac gcagtggcag gcgcgggttt
      121 tccagcacga tcttcgtcga ttgcggggcg cgcgacaaag tctgcgccag aaccgcattt
      181 cgcgtgcgaa caaagaaata gcgaatcggc gtctgtggtc cctcggtttc atcaagagcg
      241 ccattttcga gcgagatcac gacgtcacgg ctcttcactt catcctccga gaccggatgc
      301 gggagagcga ggcttgactt gccggtggtg cggttcttga tcttgaccag aagaatgtcg
      361 ccgcggctga tcggcccgag agccgagatg tcgaccgtgg tcgccgtttc ccctttgttg
      421 tgtttgcagg gcatgtcgat ctcggctgaa cgggaagtgc gcagattgcg cacggtcagc
      481 gtgcagttgg ttgggcaacc cgtgggattt tccttgccga gtgagcaggg aaaggtcagc
      541 gagagcgcaa accccgtcgg agctgcggtg gcgggaaggg aagtcgtgtg gggcaacaga
      601 ttgtcgcctt tcatccaggt ttcgagttgt gtcttggctt cctggtagtt cgggttgatc
      661 gccagcgcac ggcggaactc ttcgaccgct tcgtcgcgtt gtccgaggag ggtgtagaag
      721 ttgccgagct tcagatgaat gtccgggtag ttcgggtgat ccttgatgat cttctgatgc
      781 atctcgatca gcgataccag acgatcgaag taatcttcga ggtgcttgag gcgagtggtg
      841 agatcgtaat agttcgggtt gatctcctcg gctgagagga aattcacctt cgactcggcg
      901 aattgtccga ccgagtagta gtagtcgccg agcatgtaga agacgcgaat gttcttcgga
      961 tgaatctctt cgagcacctg aatggcttcg tgaaacagtt cctgttttcc cagaaccgtt
     1021 gccaggtgca tcttggcttc gagatactgt gggttgagtg ccagagcctt gtgataattg
     1081 tcgacggctt tctgccaatc gcgatggagt tcccacaatc tgccgaggtc gttgtagaga
     1141 tcaggatacc gggtcttttc cccgatctgg ctttcgacca ggttcacggc tttcttgaac
     1201 aacgcatcat cgttggtgat ctcggccagg aagcccatgg ccatgtagtt ttcggggtca
     1261 agatgaatgg cctgcttgag cacgtcgatc gcctgcgaga agttgtgctc accatagtga
     1321 atttcgccca ggatctgaaa gatactcgcg gaccagttgc agtaggtaag aagctgctgc
     1381 gtctgctcgc gggcttcctc gaggttgccg tagagaacgt tggtacggat gtacttcagc
     1441 agtgccgaaa gatcgctttc gagaatcttc ggcgaggtct gtttcttccc gccctgggat
     1501 tcgagcagtt gctctgaggc ggaagcgtcg ttggtctgcg gtttggattc atcgtaaaag
     1561 aggcgaaaga agaacccttt attggtttct gtctgcggtg ctcgcacctg ctctttcagc
     1621 agactgcgaa taatctggcg cagagagtgg cagtatgcac agttcggttg cagctggtat
     1681 gccgttttgc cgtaatcgat gccggtacaa tcgagattgg ccaaagcacc gtagagggga
     1741 aaccgaaaca ggctgcggta acgctcgggt tgaaactcag acccaccgcg gtgggcgaac
     1801 aggcagaagc gatcttcgtt gtaatccagt ttcccgagcc cttgaaaata gcgtgtcgct
     1861 cccttgatca tgcgcacggt ttcgagattc acgagaaaaa tcacccggtc ggcaagatca
     1921 atggcgttca acaggttctc gttgatcggg ctgtcggtat cgagaatgat gtaatcgaaa
     1981 aacttgcgca acgccttaac catcgaaatc agaatgccgg gtcggatttt ttccccgtcg
     2041 acgatgttgt tgggagccgg gagagagaaa aaactctcgg catggcgtac cagtccgcga
     2101 gagagggttt cccacgcctg ttcacctttt tcgaccaact gacacatttc gatgactgac
     2161 ttgggcgaga cgatgcccag caggtaggtg gaatctgcaa aggaaaaatc gaggtcgatc
     2221 aagagtaccc gtcgtgaggt ttcgtgagcg agaatctgcg ccagtgttgt ggcgaagata
     2281 ctcttgcccg tgccgttgcg cggggaagcc accgtgataa tgcgggcttc ggtcggggtt
     2341 ccggccgtca ctatgctgct gctaccagca acagctttct gggacgtgtc tttcagccgc
     2401 ttgctcaggg cattcaagag cttgaagctg atcgagggtt tttcctgaag cagacgctcg
     2461 aacccggtgc cgggaataat gatcagggtg gagttctgga gcgcgcgtgc cgtggctgag
     2521 cggacggaat ccttatcaaa cagcgccatc tcgccgaaaa aatcgccgtt gcgcatgacg
     2581 gcgacctgct tcagatcctg tttgtagtct ttcaacagaa tctcgacctg gccgttctgg
     2641 acaagaaaca gatcgcgggc gaaatctccc tgtgaaaaaa tcacttctcc gcgcagcaca
     2701 ctgcgggtaa gcgcgatctg ctcgatatcc ctcagttctt tcgtgctcag actagaaaat
     2761 agatcgatct gttccagcat gcatcctctc cagatggctt gtcatttcca gaaatcggaa
     2821 cctgcagtgt agcaaaggca gacagaatt
//
LOCUS       DAORHD010000587         2841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41317_length_2841_cov_3.374013, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000587 DAORHD010000000
VERSION     DAORHD010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41317_length_2841_cov_3.374013"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKO06_12570"
     CDS             <1..677
                     /locus_tag="PKO06_12570"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNV70526.1"
                     /translation="MPCTAKKYESLRPEMSIKGLREVDLVITTREFVRLVTEAGLDFP
                     HLPEEPFDDPLGVSSGASQRFGASSGVARAVACELASSDGVIPVECTDDGVERFDIRI
                     PADPACQHPELSIGFAVVSGLARARSFVQHWKEGRYPGVHYVEVMCCPGGCVGGGGQI
                     QPTDDQRIVLRSSALQAHGERLALRRARDNPAVQRLYREFLGEPYGDKSRELLHTRYT
                     ARSRFW"
     gene            711..2102
                     /gene="hydG"
                     /locus_tag="PKO06_12575"
     CDS             711..2102
                     /gene="hydG"
                     /locus_tag="PKO06_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HNV70527.1"
                     /translation="MAATTYSEVIREAEIEHILHQSQKFDAKRFEEILGRAAMKKGLP
                     FEDVAFLLALTDRDAISRMTELAMRVKEDIYGKRLVLFAPLYTSSYCRNECLYCGFRT
                     KGETARKRLSLQEVSQEAEALVQMGHKRVLMVAAEDPQLSSEMIRRQVEAIYSVKLPK
                     GNIRRVNVNVAPMEEADFQEIKKAGIGTYQCFQETYHRETYKRMHLRGSKADYDYRLH
                     VFDRCIPAGIDDFGMGILFGLYDYRFEVLALLQHVAFLEKTFGIGPHTISIPRLEPAE
                     GTNVYETSPHIISDQDLIKATAILRLAVPYTGIILSTRESIAMRETLLNVGVSQMSAG
                     SITNPGGYSEKEENVPQFQVQDHRSLDEVIRAACNAGFVPSFCTGCYRKGRTGEAFQD
                     LARHEHISPFCTVNAFTTLLEYLEDFASDETKRAGETCIRRYLDNLCDSKLKLQIEER
                     LRQIRDGKRDLLY"
     gene            2115..>2841
                     /gene="hydE"
                     /locus_tag="PKO06_12580"
     CDS             2115..>2841
                     /gene="hydE"
                     /locus_tag="PKO06_12580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HNV70528.1"
                     /translation="MSVQVQNIQKLLAELATRRPLTSEEAAWIFSAEFDAHRSLLYET
                     ADRIRKERVGDDVYLRGLIEFSNYCACRCSYCGISALNTEVSRYRMTVEEIVQNVKEA
                     VGIGYRSFVLQSGEDRHFKAADICRLIESLKQVEDIAVTLSIGEWKREDLAAFRAAGA
                     DRYLLRIETSDPDLFARHHSDSSWHDRHRCLMDLKELGFQTGSGIMVGLPGQTAETLR
                     RDLEYLVQLRPEMVGIGPFIPHPD"
BASE COUNT          596 a          749 c          872 g          624 t
ORIGIN      
        1 tcatgccgtg tacggcgaaa aagtacgagt cgctgcgccc tgagatgtcg ataaaaggcc
       61 tgcgagaagt tgatctggtt atcaccaccc gcgaattcgt tcgcctcgtg accgaagccg
      121 ggctcgactt tccccatctc ccggaagaac cattcgatga tccgctcggt gtctcttcgg
      181 gggcctcaca gcgctttggt gccagcagcg gtgtggcccg tgccgtcgcc tgcgaactgg
      241 catcaagcga tggggtgatt ccggtggaat gcactgatga tggggtcgag cggtttgata
      301 ttcgcattcc cgcggatcct gcctgtcagc acccggaact gagcattggt tttgcggttg
      361 tcagtgggct ggcgcgtgcc cggtcgttcg tccaacactg gaaagaaggt cggtatccgg
      421 gcgtgcacta tgtggaagtc atgtgttgtc cgggagggtg tgtcggaggg ggcggtcaga
      481 ttcagcccac ggacgaccaa cggattgttt tgcgctcatc tgctcttcag gctcacggtg
      541 agcgactggc actgcgccgc gcccgggaca accccgcggt gcagcgactg tatcgagagt
      601 ttctcgggga gccctatggc gacaagagtc gcgagctcct gcatacgcga tacacggctc
      661 gttcgcgttt ctggtagaag cgacgaacca gcacggaaaa ggaggccatc atggctgcga
      721 caacgtattc tgaagtgatc agggaagccg agatcgaaca cattctgcat caatctcaga
      781 agtttgacgc gaaacgattc gaagagattc tgggtcgagc ggcgatgaag aaagggctgc
      841 cctttgagga tgtcgccttt ctgctggcgt tgaccgatcg tgacgcgatc agccgtatga
      901 ccgaactcgc catgcgggtg aaagaagata tttacggaaa gcgactggta ctgttcgcgc
      961 ctctgtacac ctcgagctac tgccgcaacg agtgcctgta ttgcgggttt cgcaccaagg
     1021 gtgagacggc gcggaagcgg ttgtcgctgc aggaagtgtc tcaggaggcg gaggccctgg
     1081 ttcagatggg gcataagcga gtgttgatgg tcgcggctga agatccgcag ctcagctcgg
     1141 agatgattcg tcgccaggtc gaggcgatct actcggtcaa gctgccgaag ggcaacattc
     1201 gtcgtgtcaa tgtcaacgtc gctcccatgg aagaagcgga ctttcaggag atcaagaagg
     1261 ccggtatcgg gacctaccag tgctttcagg aaacctatca ccgggaaacc tacaagcgga
     1321 tgcatctgcg cggcagcaag gcagactacg attatcggct gcacgtgttt gatcgatgta
     1381 ttcccgctgg cattgatgat ttcggtatgg gcattctgtt cggcttgtac gattatcgct
     1441 ttgaggttct ggctctgttg cagcatgtcg cctttctgga aaagaccttc ggtatcgggc
     1501 cgcacacgat ctcgattccg cgccttgaac ccgccgaagg aacaaacgtg tatgagacct
     1561 ctccgcacat catttctgac caggatctga tcaaagccac cgcgattcta cgtctggcgg
     1621 tgccttacac cggcattatt ctttcgacgc gcgagtcgat cgccatgcgt gaaacgctgt
     1681 tgaacgtcgg agtgtcgcag atgtcggcag gttcgatcac caaccccggt ggctactcgg
     1741 aaaaggaaga gaatgtgccg cagtttcagg tgcaggatca ccggtcactg gatgaggtca
     1801 ttcgggcggc ctgcaatgca ggtttcgtgc cgagcttctg taccggctgc tatcgcaagg
     1861 gacgcacggg ggaagccttt caggacctgg cacggcatga acacatcagc ccgttctgca
     1921 cggtgaatgc gtttacgacg ctgctcgaat acctcgaaga ttttgcctcc gacgaaacga
     1981 aacgagcggg agaaacctgc attcgtcggt atcttgacaa tctctgcgac tcgaagctga
     2041 agctgcagat cgaagagcgg ctgcgccaga ttcgtgacgg caaacgcgat ctcttgtatt
     2101 aaaaggagcg ccacatgagc gtccaggttc aaaatataca gaaactactc gcagagctgg
     2161 ctacgcgcag gccactgacc tcggaggaag ctgcctggat attctcggcg gagttcgatg
     2221 cccatcggtc attgctgtat gaaacggccg atcgtatccg caaagagcgc gtcggggacg
     2281 atgtgtacct gcgtggtttg atcgagttca gcaactactg cgcctgtcga tgttcctatt
     2341 gtgggatctc ggctctgaat accgaagtca gccgatatcg catgacggtg gaagaaatcg
     2401 tgcagaatgt gaaagaggcc gtgggaatcg ggtaccggtc gtttgtgctg cagtcgggtg
     2461 aagatcgcca tttcaaggcc gcggatatct gccgcctgat cgagtcgctg aagcaggttg
     2521 aggatatcgc cgtgaccctc tcgatcgggg aatggaagcg cgaagatctg gcggcctttc
     2581 gggccgcggg agccgatcgc tatctgctgc gcatcgaaac gtctgatcca gatctgtttg
     2641 cccgacatca ttccgattcc agctggcacg atcgtcatcg ctgtctcatg gatctgaagg
     2701 aactcgggtt tcagaccggg tcgggcatca tggtcgggct ccctggtcag accgcggaaa
     2761 cgctgcgtcg tgacctcgaa tatctggtgc agttgcgtcc ggaaatggtg gggatcggcc
     2821 ccttcattcc acatcccgac a
//
LOCUS       DAORHD010000588         2840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41329_length_2840_cov_4.871454, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000588 DAORHD010000000
VERSION     DAORHD010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41329_length_2840_cov_4.871454"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="PKO06_12585"
     CDS             complement(<1..1444)
                     /locus_tag="PKO06_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV70529.1"
                     /translation="MDASLNIVSRLLEKGHHIDDLKMLLSPFRERGFDEFAEQMAIML
                     SKVSALLEISNELSETLSLDTLLHRIIQIATEALDADRGTLFLYDAPHKELFSRVLQG
                     DSVQEIRFPCHLGIAGAVFTSGEPIIIHDAYSDPRFNKEIDRKTGYHTRNILCAPIKT
                     RHGAAVGVIQLLNKAENRGTFNQDDLVLLRSITSQASAALQNAQLFEQVQKARAEQER
                     LLELSTAISSELQLSSLLAKIMETTTIILNAERSTLFLHDARTNELWAPVAQGMDGGK
                     EIRFPSHLGIAGSVFTSGNTVNIPDAYADPRFNKDFDRKTGYVTRNILCMPVINKSGK
                     VIGVTQVLNKRGGPFARSDEQKLAAFSAQASIAIENARLFEDVMNMKNYNESILQSMS
                     NALITLDGTDHIVTANAAAGRMLQCDTASLLSQTPTNLFGSANEWLLKLLGSVAQSKK
                     PEHLLDSEIQLPNNKHVSVNASAVPLISIKQ"
     gene            1571..2239
                     /locus_tag="PKO06_12590"
     CDS             1571..2239
                     /locus_tag="PKO06_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HNV70530.1"
                     /translation="MTNSERTLRVGAARDAIEMTSHAIAEHLRRADQTLAQVISHVGL
                     LKIEPSRLPHPFQTLFHAIVAQQISTRAADTIVRRVHALFPRHRILRPDEILAIPEVQ
                     LRLAGLSFKKIAAVKDLADQALSGALPTVKRLLTLTDDEVRTCLTRIRGIGVWTADMF
                     LIFHLQRPNVLPLSDHALKRSVMLAYELPELPSNEELLVISERWRPFRSVACRYLWRY
                     LDSL"
     gene            complement(2240..>2840)
                     /locus_tag="PKO06_12595"
     CDS             complement(2240..>2840)
                     /locus_tag="PKO06_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70531.1"
                     /translation="RNLACSIRSALRLYLLLLAFLSPGMIEAVGNAVPLPGSSAPAVP
                     SVGAGGMTWKYKRASGPMKFGGDLQMIGLRLFGGRKDDPKVGLAASTGTLSGSALQLE
                     VRMGGITLEDSFRTDRNLKWRATLGAGRYDLHTRRTGFTVNSGGFAFFEPMFIGVIPM
                     NKHISIELGGGYTFAGTTGVKLEGPFFEFNLLLGRNLEP"
BASE COUNT          580 a          788 c          845 g          627 t
ORIGIN      
        1 cctgcttgat gctgatgagc ggcaccgcac tcgcgttgac cgagacgtgc ttattgttgg
       61 gcagctggat ctcggaatcg agcagatgtt caggtttctt gctctgcgct accgaaccaa
      121 gcagtttcag caaccactcg ttcgcgctgc cgaacagatt cgtgggggtc tgggacaaca
      181 gcgacgcggt atcgcattgc agcatgcgac ctgccgcggc attcgccgtc acgatgtgat
      241 cggtgccatc gagagtgatg agagcgttgc tcatactctg cagaatgctc tcgttgtaat
      301 tcttcatgtt catcacgtct tcgaacaagc gggcgttctc aatcgcaatg ctggcctgag
      361 cgctgaaggc cgcgagcttc tgctcgtcag accgggcgaa cggaccgccg cgtttgttga
      421 gaacctgcgt gacgccgatg actttgccag acttgttgat gaccggcatg cacaggatgt
      481 tgcgggtgac atagccggtc ttgcgatcga aatccttgtt gaaacgcggg tcggcatacg
      541 catcgggaat gttcacggtg ttcccgctgg tgaacacgga tccggcgatt cccaggtggg
      601 acggaaagcg aatctccttg ccgccatcca tgccttgggc gacgggagcc cagagttcgt
      661 tggtgcgtgc gtcatgcagg aacagtgtgc tgcgttcggc gttgagaatg atcgtggttg
      721 tttccatgat cttggccagc agcgatgaaa gttgcagttc agaagagatg gccgtggaaa
      781 gttcgagcag gcgttcctgt tcggcgcgtg ccttctggac ctgctcgaac agttgggcgt
      841 tctgcagggc agctgaagcc tgtgaggtga tcgagcgcag caggacaagg tcatcctggt
      901 taaaagtgcc tcgattctcg gccttgttca gcagctgaat tacaccgacc gcagctccgt
      961 ggcgggtctt gatcggggcg cacaaaatat tgcgggtgtg atacccggtc ttgcgatcga
     1021 tttccttatt gaacctgggg tccgagtagg cgtcgtgaat gatgatgggc tcgccactgg
     1081 tgaagacggc accggcgatg cccaggtggc acggaaaccg gatttcctga acgctgtcgc
     1141 cctgcagaac ccgggaaaac aactctttgt gcggggcatc atacagaaag agcgtgccgc
     1201 ggtcagcgtc gagtgcctca gtcgcaatct gaatgatacg atggagcagt gtgtcgagtg
     1261 agagcgtctc ggaaagttca ttggaaatct cgagtaacgc tgacaccttg ctcagcatga
     1321 tggccatctg ctcggcgaac tcgtcgaacc ctcgttcgcg gaatggtgaa agcagcatct
     1381 tcaggtcatc gatgtgatga cccttttcca gcaggcggct gacgatgttc agactggcat
     1441 ccatactcat ctcacccggt gtgtaccggc attggcgaat tttctatctc tcatctttac
     1501 catgaagacg agcgattgtc gatagtcgat ccggaaaatc gcacgtctgt ggtatccttc
     1561 tggtagagcc atgacaaact cggaacgcac tcttcgagtc ggggccgcgc gcgatgcgat
     1621 cgagatgacg agtcacgcta tcgccgaaca cctgagacgg gccgatcaga cgctggcaca
     1681 ggtcatttcc catgttggtc tgctgaagat cgaaccgagt cggttgccgc atccgtttca
     1741 gactctgttt cacgcgatag tcgcccaaca gatcagcacg cgtgccgccg atacgattgt
     1801 ccgacgtgtg catgcgctgt ttccgcgcca tcgaatcctg cggccagatg agattcttgc
     1861 gattcctgaa gtgcagttac gcctggcagg gctttccttc aagaaaatcg ccgccgtcaa
     1921 agatctggca gaccaagcgc tgtcgggcgc tctgccaacg gtgaagcgat tgctgactct
     1981 cacggatgac gaagttcgaa cctgcctgac ccgcatacgg ggaattggcg tctggactgc
     2041 cgatatgttc ctgatctttc atctgcagcg cccgaatgtg ctgccactgt cagatcatgc
     2101 cttgaagcga agcgtcatgc tggcatacga attaccggaa ctcccctcga atgaggaact
     2161 gctggtgatc agcgagagat ggcgcccctt tcgatcggtt gcctgccggt atctctggcg
     2221 ttatctcgac agcctctgat cagggttcga ggttgcgacc gagcagcaga ttgaactcaa
     2281 aaaacggccc ttcgagtttg acaccggtcg tgcccgcaaa ggtgtatccc ccgccgagtt
     2341 cgatcgagat gtgtttgttc attggaatca caccgatgaa catgggttcg aaaaacgcga
     2401 agcctccgga gttgaccgtg aagccggtac ggcgcgtgtg aagatcgtag cgcccggctc
     2461 caagtgttgc ccgccacttc aggtttcggt cggtgcggaa cgaatcttcg agcgtgattc
     2521 cacccatgcg cacctcgagt tgcaacgcgc ttccgctcag ggtaccggtg ctggccgcga
     2581 gcccgacctt cgggtcgtcc ttgcgcccgc cgaacaggcg cagaccgatc atctgcagat
     2641 ctcccccgaa cttcatcgga ccgctggcgc gcttgtactt ccaggtcatg ccgccagccc
     2701 cgaccgaagg aacagcagga gcggatgaac ccggcagggg aacggcgttc ccaacagctt
     2761 cgatcatgcc agggctcagg aatgcgagca gcaacagata gagccgcagc gcacttctga
     2821 tcgagcaggc aaggttccgg
//
LOCUS       DAORHD010000589         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41364_length_2839_cov_3.606322, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000589 DAORHD010000000
VERSION     DAORHD010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41364_length_2839_cov_3.606322"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..796
                     /locus_tag="PKO06_12600"
     CDS             155..796
                     /locus_tag="PKO06_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193567.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV70532.1"
                     /translation="MARESHLIESFEQALTGLDRLAAGQIFQALLQTRSVMDGVEALI
                     VPTLDRIGTSWEQGRLSLAQVYMSGRICESLVEGHLKVQPLPPRPQPKLALAVLEDQH
                     LLGKRMILSCLLAAGYRVHDFGPLEVEPLVRRTVEEGIECLLVSVLMLPSALRVKNVC
                     EGLKAKGVSVRVVVGGAPFRFDPHLWQEVGADAVGCSASEAVSIVNRFSGAAS"
     gene            793..1833
                     /locus_tag="PKO06_12605"
     CDS             793..1833
                     /locus_tag="PKO06_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNV70533.1"
                     /translation="MSNLKMTSLQRVLTALSHQEPDRVPFFLLLTMHGATLHGVSIRE
                     YFSQPELVADAQIRLREKFGHDCLYAFHYAAVECEAWGAEVIYRDDGPPNTGEPFIRR
                     PDDIRRLACPDVSAAPSLTKVCRAIRLMKERIGDQAPIIGVVMSPFSLPVMQMGFESY
                     IELMYEQPDLFERLMQINEDFCVAWANAQLQAGATAICYFDPVSSATIVSPDLYRKTG
                     CEIACRTIARIKGPTATHFASGRCLSIVDDVARTGTAVVGVSVEESLAQLKQKTRGKL
                     SLLGNLNAIAMRRWTASEAERQVREIIAQAAPGGGFIISDNHGEIPLQISDDVLMSIA
                     ETVRRYGTYPIR"
     gene            1841..>2839
                     /locus_tag="PKO06_12610"
     CDS             1841..>2839
                     /locus_tag="PKO06_12610"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNV70534.1"
                     /translation="MTPQVDGILVCEILRPEVVAALADSDYRDLRILSFPTYCQHRGN
                     RTCKLDQHIRLALPQVGQLLVFAGTCHHPQEPQAALPPGVKVVSVNNCFQHFLPQPLL
                     EKLLREGAYLVTPGWLAVWRKVLETWGFDQPLAREFFRESCRRVVLVDTLIDPLSEQK
                     LSEFGSYIDRPVETVPVGLDFFRLRLRELLRGRPENRAEPPARSLAEIAVVFDILNDL
                     VGVKDERQAVEAIFRFVQTISSPRFMAFMIVRDSREYAVHTSPELAPIDDDTRRQMVQ
                     CENDHVTTVEDGFLIKIHRGGERLGVLLVKGFASPRHKYEYLNLLLLVQPALALAVV"
BASE COUNT          531 a          768 c          881 g          659 t
ORIGIN      
        1 gcggtggggg caggccgcaa tcactgtgct cgacgacgat ggactcgaaa tcggtcgtga
       61 agagtgatag tattcgaagg cgagagagct tttgtcccgt ttcaatggat caatgaggcg
      121 actgggtgga ccggttcgcg aaaggaggaa atctgtggcc agagaaagcc atctgatcga
      181 aagctttgaa caggctctca ccgggcttga ccgactcgcg gccgggcaga tctttcaggc
      241 tctgctgcag acgaggtcag tcatggatgg agtcgaagcg ctgattgttc cgaccctgga
      301 ccgcatcgga acctcgtggg agcagggccg tctttcactg gcccaggtgt acatgagcgg
      361 gcgtatctgc gaatcgctgg tcgaaggtca tctgaaagtg caacctctgc cgccgaggcc
      421 gcaacccaag ctggcgctgg ccgtgctcga agatcagcat ctgctgggaa agcggatgat
      481 actgtcgtgt ctgctggcgg cggggtatcg agtgcatgat ttcggccctc tcgaagtcga
      541 gcctctggtg cgtcgcaccg tcgaagaggg tatcgagtgc cttctggttt cggtgttgat
      601 gctgccttcg gcgctgcgag tcaaaaacgt ctgcgagggg ttgaaggcga agggagtgtc
      661 tgtccgcgtt gtggtgggtg gtgcgccgtt tcgctttgac ccgcacctct ggcaggaagt
      721 gggggctgat gcggtcggtt gttcagcgtc agaggcggta tcgatcgtga atcgtttctc
      781 gggagccgca tcatgagcaa cctcaaaatg acttctctgc aacgagtgct cacggccctg
      841 agtcaccagg aacctgaccg ggtgccgttc ttcctgctgc tcaccatgca tggggccacg
      901 cttcacgggg tgtcgatccg tgagtatttt tcgcaacctg agcttgtcgc tgacgctcag
      961 atcagattga gagaaaagtt cgggcacgat tgcctgtatg cgtttcacta tgcggcggtc
     1021 gagtgtgagg cgtggggtgc cgaagtgatc tatcgtgacg acgggccgcc gaacaccgga
     1081 gaaccgttca ttcgccgacc tgatgacatt cgacgactcg cctgtcccga tgtttcagcc
     1141 gcgccaagcc tgacgaaagt ctgccgggcc attcgcctca tgaaagagcg catcggcgat
     1201 caggcaccga tcatcggggt cgtcatgtcg ccgttctctc tgcccgtcat gcagatgggt
     1261 tttgaatcgt acatcgaact gatgtacgaa cagcctgatc tcttcgagag attgatgcag
     1321 atcaacgagg acttttgtgt tgcctgggcc aatgcccagt tgcaggctgg ggcgactgcc
     1381 atctgctatt ttgatccggt ttcgtcggcg acgatcgtct cgcccgatct gtatcgcaag
     1441 accggttgcg agattgcctg tcgaaccatt gcgcgcatca aggggccgac ggcgacgcat
     1501 ttcgcttcgg ggcgctgcct gtcgattgtc gatgatgtgg ctcgtactgg taccgccgtc
     1561 gtgggagtca gcgttgaaga gagtctggcg cagttgaaac agaagacccg cggcaagttg
     1621 tcgctgctgg gaaatctcaa cgccatcgcc atgcgccgct ggaccgcatc ggaggctgaa
     1681 cgacaggttc gcgagatcat cgcacaggcg gcaccgggtg gagggttcat catctctgac
     1741 aatcatggcg agataccgct gcagatttct gatgacgtgc tgatgagtat cgccgaaacc
     1801 gttcgccgat atggaacgta tccgattcgg tgagacagcc atgacaccgc aggtcgacgg
     1861 aattctcgtc tgtgaaattc tgcgccctga ggtggttgcc gctctcgcag actcggacta
     1921 tcgcgatctc agaattctgt cgtttccgac ttactgccag catcgcggca atcgcacctg
     1981 caagctcgat cagcacattc gtctcgcgct gcctcaggtc ggacaattgc tggtctttgc
     2041 cggcacgtgt catcacccac aggaacccca ggctgctctc ccgcccggtg tgaaagtggt
     2101 ttcggtcaac aactgctttc agcattttct gccgcaaccg ctgctcgaaa agctgctgcg
     2161 cgagggggcc tacctggtta ctcctggttg gctggctgtc tggcgaaagg ttctcgaaac
     2221 ctggggcttt gaccaacctc tggcgcgtga attctttcgt gaatcgtgcc ggcgcgtggt
     2281 tctggttgat acattgatcg atccgctttc tgagcagaaa ttgtccgagt tcggatcgta
     2341 catcgatcga cccgtcgaaa cggtcccggt cgggctcgac ttctttcgtc tgcgtctgcg
     2401 cgaacttctg cgtggccggc ctgagaatcg ggccgaaccg ccggcgcgct cgctggccga
     2461 gattgctgtg gtcttcgaca ttttgaatga tctggtcggc gtgaaggatg agcgacaggc
     2521 ggttgaagcc atcttccgct ttgtgcagac catcagttcg ccccgcttca tggcgttcat
     2581 gatcgtacgc gattctcgtg agtatgccgt tcatacctcg ccagaacttg cccccattga
     2641 tgatgatacg cgaagacaga tggtacaatg cgagaatgac cacgtgacca cggtcgaaga
     2701 cggtttcctg atcaagattc atcgcggggg agagcgtctc ggggtgctgc tcgtgaaggg
     2761 cttcgcgtcg cctcggcata agtacgagta tttgaacctg ttgctgctgg ttcagccggc
     2821 acttgcgctg gccgtggtg
//
LOCUS       DAORHD010000590         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41421_length_2837_cov_3.289001, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000590 DAORHD010000000
VERSION     DAORHD010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41421_length_2837_cov_3.289001"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1682)
                     /locus_tag="PKO06_12615"
     CDS             complement(198..1682)
                     /locus_tag="PKO06_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70535.1"
                     /translation="MNSKRPASRPTSPRDNRHSPEDKRTSERSAGSNSPWNTRAARTT
                     TDETRVPFAPGPSRDGPGSPRFCFAGEVASPTSWGRIVREILLAARDLEMPLALRPIK
                     GYAFEAEYPLPDFLARLSERPPLGKIQIAVQPAFCLHLPEPRTTRWACTSFCLPPTPA
                     EVKNLQGFHQIWVFSEDHRQALARAGIPLERIRALPLAVHPKMFHLRAPRHPDIPRDN
                     RFRFLYVGNPLRRKAVDLVLRAYLEEFRTAEPVELVIKLTHLPRIKKQFDYEIADLAK
                     RLGAINNLFPRVRVITGCWEDKDLAGLMTACQTLVSADRAYFTALVVREGMACGVPVI
                     GPAILGPLAGLTAETGWFVQTREVEYEPGILFPGSPKTRGEEVDIADLRRLMREAFTQ
                     PGATRKKGVEAARAVAQAREWRETAERLRAEGARTAEELARRRAESAPPSGGPGHSSG
                     PSSSHAGSRRTSGDRPGERSTRGSYKAGMRPSKPRGGREGDRRD"
     gene            complement(1679..2548)
                     /locus_tag="PKO06_12620"
     CDS             complement(1679..2548)
                     /locus_tag="PKO06_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV70536.1"
                     /translation="MTVAVVADLHTHSNASDGVLAPAELVQKAAGAGLRALALTDHDT
                     LNGIPEALTAGAQSGLQIIPGIELSCGWADREPSLHMLGLFIDHRATGLNELLDQQRR
                     GRKTRALQILDKLEGLGVPVQPLRESFLREQAQALGRPHIARYLVDIKAIQDFQEGFR
                     RYLGRGCPAYVPKPHVLPEDGIRLVHQAGGIACIAHPGLVPDWDAIWPRIAGLPWDGI
                     EAVYAEHTPSQVERFTALARSQNWLITGGSDYHGEAGKHVARLGEQGLTDEQFQQLAE
                     AARQRLSRRGELT"
BASE COUNT          534 a          880 c          880 g          543 t
ORIGIN      
        1 ccaagcgcga ctggaaaggc accggtctgg ggctggccgt gtcatttcgc atcatccagc
       61 tgttcaaggg ttcgctcgcc gtgcaaagca cggtgggtgt cggatcgcgg ttcaccatca
      121 ccgtcccgtt gtatcaacgg ccgacctgaa gcccgcagcg acgccgcggc cggcgtcgcg
      181 tgtatcagtc ccgcgtgtca atcgcgtctg tcaccttctc ggcctcctcg tggtttcgag
      241 ggacgcatgc cggctttata tgaccctctc gtcgatcttt cacccggacg atcgccagaa
      301 gtgcggcgcg agccggcgtg agaagacgat gggccggacg agtgaccagg accgccagat
      361 ggcggtgccg actcggcacg tcgtcgtgcc agttcttcag ctgtccgggc accttctgca
      421 cgcagcctct ctgcggtctc tcgccattcg cgggcctggg ccacggctcg cgcggcctcg
      481 acccctttct tccgggtcgc gcccggttgg gtgaaggctt cccgcatcag acggcgcagg
      541 tcggcaatat ccacttcttc gcctcgagtt ttcggcgagc cgggaaacag gattccaggc
      601 tcgtattcga cttcacgagt ctggacaaac cagccggtct cggcggtcaa ccccgccagc
      661 ggtcccagaa tcgccggccc gatgaccggg acaccacagg ccatgccttc gcgcaccacc
      721 agcgcggtaa agtaggcccg atcggccgag accagcgtct gacaggccgt catcaacccc
      781 gccagatcct tgtcttccca gcatccggtg atcacgcgca cccgcggaaa cagattgttg
      841 atcgcgccca ggcgtttcgc cagatcggcg atctcatagt cgaactgctt cttgatgcga
      901 ggcagatggg tgagtttgat cacgagttcg accggttccg ccgtgcgaaa ctcttcgaga
      961 taggcccgca gtaccagatc gaccgccttg cgtcgcagtg gatttccaac atacaggaaa
     1021 cgaaatcggt tatcgcgtgg aatgtcaggg tgacgcggcg cacgcagatg aaacattttc
     1081 ggatgcaccg cgagcggcag cgcgcggatg cgctcgagcg gaattcccgc gcgggcaagc
     1141 gcctggcgat gatcttccga aaagacccag atctggtgga agccctgaag gttcttgacc
     1201 tcggccggag tcggtggcag gcagaacgat gtacaggccc atcgggtggt gcgaggctca
     1261 ggcagatgca gacaaaacgc cggctgcacg gcgatctgaa tcttgccgag cggcggacgt
     1321 tcgctcaagc gcgccagaaa gtcaggcaat ggatattcgg cttcgaaggc gtagcctttg
     1381 atgggtcgta acgccagcgg catctcgagg tcgcgcgccg ccagcagaat ctcgcgcacg
     1441 atgcgccccc acgaggtggg cgaggcaact tcgccggcga aacagaagcg agggctgccg
     1501 ggaccgtcac gcgagggtcc gggggcaaaa ggcacgcggg tctcgtcggt ggttgtacga
     1561 gctgctcgag tgttccacgg gctgtttgag ccggcagaac gctctgacgt tctcttatct
     1621 tcaggggaat ggcggttgtc acgaggcgag gtcgggcgtg aggcaggtcg tttcgagttc
     1681 atgtgagttc tcctcggcgc gagagtcgct gacgagcggc ttccgccagt tgttgaaact
     1741 gttcgtcggt caggccctgt tctcccagac gtgccacgtg tttgccggct tctccgtgat
     1801 agtcgctgcc gcccgtaatc agccagttct gacttcgggc cagagccgtg aaacgctcga
     1861 cctgcgaggg cgtgtgttcg gcatagaccg cctcgattcc atcccacggg agaccggcaa
     1921 tgcggggcca gatggcgtcc cagtcaggta ccagcccggg atgagcaata caggcgatac
     1981 caccggcttg gtgaacgaga cgaatgccat cttcaggcaa gacatgcggt ttgggcacat
     2041 aggcggggca ccctcgcccc agatagcggc gaaacccctc ctgaaagtcc tgaatcgcct
     2101 tgatgtcgac gagatagcgt gcaatgtgcg gtcggcccag agcctgcgcc tgctctcgca
     2161 gaaacgactc gcgcaagggc tgcacgggaa cgccgagccc ttcgagtttg tcgagaatct
     2221 gcaacgcgcg ggtcttgcgg ccgcgacgct gctgatcgag cagctcgttc agaccggtcg
     2281 cccggtgatc gatgaacaac ccgagcatgt gcaatgaggg ctcgcgatcg gcccagccac
     2341 aggacagctc gatcccggga atgatctgca gcccggactg tgcgcccgcg gtcaacgctt
     2401 cggggatgcc gttgagcgta tcgtggtcgg tgagcgcgag cgcgcgcaac cctgctccag
     2461 cagctttctg tacgagttcc gcgggagcaa gaactccgtc tgatgcattg gaatgagtgt
     2521 gtaaatcagc aacaactgcg acggtcatca cgaacggcgt cctttgcggt cagggtgaaa
     2581 ctgcggtccg ttccgcttca tcacggtatc agcaccgtca agaccgaagc cggaccagcc
     2641 ggcatcttac ttcactcttt cccattccgt caaacgaatg cggcgacagt atactctatt
     2701 cacaacccac aaacgcgtat actgtcgccg cattgatttg acacgccgtc ggcgatcgac
     2761 tatacttcgg cgcatgaaac acctaccaac actcgccctg gttcggggtt ttcccgtgat
     2821 cttccttgcc gcgctgt
//
LOCUS       DAORHD010000591         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41465_length_2835_cov_4.202158, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000591 DAORHD010000000
VERSION     DAORHD010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41465_length_2835_cov_4.202158"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PKO06_12625"
     CDS             complement(<1..723)
                     /locus_tag="PKO06_12625"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HNV70537.1"
                     /translation="MLPTPQQQLLEHAEVIRSVDALEAFLGEVHDCDVFAVDTESAGF
                     YKYYAVVNLVQISTRTHAAILDPQAVKDLSPLKRFAEQTRCEWIFHGGDYDLRMLAHD
                     FQIRVQNVFDTRIAAELCGLEHLSLSSLAEQFLGLKLDKKLQRCDWSRRPLTPDMIRY
                     GLLDAICLVPIRDCLSELLHAKNRFEWASEEFKHLEKILANPTPPQPRPYPFLIKGSN
                     RLSPRSLAVLKEVWEFRERISAE"
     gene            complement(784..>2835)
                     /locus_tag="PKO06_12630"
     CDS             complement(784..>2835)
                     /locus_tag="PKO06_12630"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70538.1"
                     /translation="PDSFTIPPARLIALDFLLQGAKDEESDLALVQAIRQSSATVILG
                     GQTTVEVEQGDETRVKAVSFASGTFKHDVLQLDQKVSVILPHQPFLDVCPNVGFINVG
                     QSKGFVRRVPLFVPDPTNRKLLPSFSLLISATALDNQSENPGRMLYQRAMYSELERIG
                     EQMRNGTYVGDFLLHDIPIPTDPQGNMEVYFFGSTQRIRESRPILPSVSFYECFDEPK
                     LEFYRTRNPQQAAKLMPQAAHLNSASAQKTRNFGNKVCLLGTFERSDFDFYETPLTLK
                     TPVPLESEQLMGIEIHANAVLTILNRKFIHPPNTMHTFILLVFATLLLGFLIDHLKPL
                     LGGLATLSGILGIFGLAYYSYQSQGQILYISPFLVSFPMSWFSSTLIGYLHQQAKALS
                     IKSMFQRFVSADVVQYMIDNPDKVAPGGQRVELTVFFSDVAGFTSISEMLTPEELVVL
                     LNEYLGAMTDILFEFGGTLDKFIGDAVMCFWNYPRAQEDHAVRACLCAIRMQEKIREL
                     QIGWAKRGLPKVSARAGINTAQVVVGYMGSEKAQMNFTCMGDGVNLASRLEGANKEYG
                     TDLMVSDACYQKAKHKVTARFLDFLAVKGKKEPVKVHNLVCEKGKEPPDWEEFISMYD
                     KAIKLHLDRQWDEAIATFEALLARWPEDGPSKTYIHRCKEYKENPPPENWDGSYHLTH
                     K"
BASE COUNT          611 a          759 c          864 g          601 t
ORIGIN      
        1 ctcggcggat atccgttcac ggaactccca cacttctttc aagaccgcca gagaacgcgg
       61 agacagacga ttcgaccctt tgatgagaaa cgggtaaggt cgtggctgcg gcggcgtggg
      121 gtttgccagg attttctcga gatgcttgaa ctcttcggac gcccattcga agcgattctt
      181 ggcgtgcagt aactcggaca aacaatcgcg aatgggaacc aggcagatgg cgtcgagcaa
      241 cccgtatctg atcatgtcgg gtgtcagcgg gcgccgtgac cagtcgcacc gctgcagctt
      301 cttgtcgagc ttgagcccga gaaactgttc agcgagcgaa ctcaagctca gatgctcgag
      361 cccgcacagt tcagccgcga tgcgggtatc gaacacattc tgcacccgga tctgaaagtc
      421 gtgggccagc atgcgcagat cgtaatctcc gccgtgaaag atccactcgc agcgagtctg
      481 ctcggcaaag cgcttgagcg gtgagagatc cttgacggct tgcgggtcga gaatggccgc
      541 gtgtgtgcgg gtcgagatct gcacgagatt gacgacggcg tagtacttgt agaaccctgc
      601 cgattccgtg tcgacggcga agacgtcgca gtcgtgcacc tcgccaagaa aggcctcgag
      661 agcgtcgacc gagcgaatga cctcggcgtg ctcgagaagc tgttgctgcg gcgtgggtaa
      721 catgggagtg tgactcggtt gatgacgttg atgacgcggt gggaacaaca ccgccattgt
      781 acactatttg tgcgtgagat ggtagctgcc gtcccagttc tcaggtggcg gattctcttt
      841 atattccttg cagcgatgga tgtaggtctt gctcggtcca tcttcaggcc accgagccag
      901 aagggcttcg aacgtggcga tggcttcatc ccactgtcgg tcgagatgca gcttgattgc
      961 tttatcatac atgctgatga attcttccca atccggtggt tccttgccct tttcgcagac
     1021 gaggttatga actttgaccg gctctttctt gcccttcacg gcgagaaagt cgagaaagcg
     1081 ggcggtgacc ttgtgtttgg ctttctggta acacgcgtca ctgaccatca gatctgtgcc
     1141 gtattccttg ttcgcaccct cgagacgtga agccaggttg accccgtcac ccatgcaggt
     1201 gaagttcatc tgcgccttct cggaacccat atagccgacg acaacctgcg ccgtattgat
     1261 gcccgcgcgt gctgagacct tcggcaggcc gcgtttcgcc cacccgatct gcagttcacg
     1321 gatcttttcc tgcatgcgaa tggcacacag acaggcgcga accgcgtgat cttcctgcgc
     1381 gcgtggatag ttccagaagc acatgactgc gtcgccgatg aacttgtcga gcgtgccacc
     1441 gaactcgaac agaatatcag tcatcgcgcc gagatactcg ttcagcaaca cgaccagttc
     1501 ttcgggggtc agcatttcgg agatcgaggt gaagccggcg acgtccgaaa agaacaccgt
     1561 cagctcgacc cgctgtccgc ctggggcgac cttgtcggga ttgtcgatca tgtattgaac
     1621 gacatcggcc gacacgaatc gctggaacat tgacttgatg ctcagggcct tggcctgctg
     1681 gtggagatag ccgatcagcg ttgacgagaa ccacgacatg ggaaagctga ccaggaacgg
     1741 tgaaatgtac aaaatctgtc cctgcgactg atacgaatag taggccaacc cgaagatgcc
     1801 gaggatgccg ctgagcgttg ccagaccgcc gagcaggggt ttgaggtggt cgatgagaaa
     1861 cccgagcagc agggtggcga agaccagcag aatgaatgtg tgcatggtgt tgggcggatg
     1921 aatgaacttg cggttgagaa tcgtcagcac ggcgttggcg tgaatctcga ttcccatcaa
     1981 ctgctcagac tcaagaggca cgggcgtctt caaggtcagc ggtgtctcgt agaaatcgaa
     2041 gtcgctgcgt tcgaaggtgc ccagcaggca gaccttgttg ccgaagttgc gtgtcttctg
     2101 cgcactggct gagttcagat gagcggcctg cggcatcagt ttcgcggctt gctgcgggtt
     2161 gcgcgtgcgg tagaactcca gtttcggttc gtcgaagcac tcgtaaaacg aaaccgaggg
     2221 cagaatgggg cgggattcgc gaatacgctg cgtcgagccg aaaaagtaga cttccatatt
     2281 cccctgcggg tcggtcggaa tgggaatgtc atgcagcaga aagtcaccaa cataagtgcc
     2341 gttgcgcatc tgctcgccaa tacgttcgag ttcgctgtac atcgcacgct ggtacagcat
     2401 gcgccccgga ttctcggact ggttgtcgag cgcggtcgcc gagatcagca gcgaaaacga
     2461 gggcagcagt ttgcgattgg tcggatcggg aacaaacagc ggcacgcgcc gaacgaaccc
     2521 tttactctga ccgacattga tgaagccgac gttcggacac acgtcgagaa acggctggtg
     2581 cggcagaatg accgagacct tctgatcgag ttgcaggaca tcgtgcttga acgttccaga
     2641 ggcgaacgag actgctttga cgcgtgtttc atcgccttgc tcaacctcga ccgtcgtttg
     2701 accgccgaga atgacggtcg cggaagactg gcggatcgcc tgaacgagag ccagatctga
     2761 ttcttcatct ttcgcgccct gcagcagaaa gtcgagcgcg atgagccggg cgggaggaat
     2821 ggtgaatgag tcggg
//
LOCUS       DAORHD010000592         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41467_length_2835_cov_3.983453, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000592 DAORHD010000000
VERSION     DAORHD010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41467_length_2835_cov_3.983453"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKO06_12635"
     CDS             complement(<1..366)
                     /locus_tag="PKO06_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV70539.1"
                     /translation="MKKTSSNIFAVILAGGSGTRLWPLSRQSWPKQLLALVGNRTLLQ
                     DTSRRILPVIPAAGQFVITSQEYAEQVQAQMSEIFPQTAPQVLAEPLARNTAPAILWG
                     ALLAREQGGKDAILAVLPSD"
     gene            complement(395..1399)
                     /gene="galE"
                     /locus_tag="PKO06_12640"
     CDS             complement(395..1399)
                     /gene="galE"
                     /locus_tag="PKO06_12640"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNV70540.1"
                     /translation="MSGNNTDRPVVLVTGGAGYIGSHACKALSLSGYQPVVYDNLIYG
                     HREFVRWGPLEEGDLADQARLREVFLKHRPVAVMHFAAFTYVGESVSDPGKYYRNNVL
                     GSLHLLEVMRETGVQMLVFSSTCATYGIPGTIPIPEDHPQHPINPYGASKLMMERMMA
                     DFSVAHGLNYLALRYFNAAGGDPDAEIGERHDPETHLIPLVLDAAMGKRPHVTVFGTD
                     YPTPDGTCIRDYIHVSDLADAHVLALEYLRRERRSGALNLGNGSGYSVKEVIATAERV
                     TGASIRVVLGARREGDPPRLVGNAVLAKSMLGWKPQYADLQVIIRHAWEYRRRGYEHR
                     "
     gene            complement(1401..2405)
                     /locus_tag="PKO06_12645"
     CDS             complement(1401..2405)
                     /locus_tag="PKO06_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV70541.1"
                     /translation="MKIILPVAGKGTRLRPHTHTKPKSLVRVAGKTVLEHVMQNVSKL
                     KAVEYIFITDENGYIIENFVKQKFPEAKATYITQSELLGPAHAVWLAKPRIEKGDDVL
                     VVFNDTLFVTDLTRIPRLVDGLDGLIYSKEVEDYRRFGVNVMKDGIIVDMVEKPDQPI
                     SRLAQVGLYYLKDGVRFMDYLTRAIDQKLTVKGEYYLPEVFKLMLKDGLKLGAPEIDE
                     WLDCGKPETLLGTNRYLLEHSTRSHYCIEGCVVIPPVAIDPSARVYHSVIGPHVSIAE
                     GCQLENVIVRDSVINANSTLKNVILDNSIIGDSAEMTGNAQRINIGDNSVIDLSTPHR
                     "
     gene            complement(2402..>2835)
                     /locus_tag="PKO06_12650"
     CDS             complement(2402..>2835)
                     /locus_tag="PKO06_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HNV70542.1"
                     /translation="QKMVLYHKAVKAFKGDLKGKTIAIWGLAFKPNTDDMREAPAIVI
                     IEHLLKAGAKVQAYDPEAMHEAKKVFGDRISYAKRSYDALNGADALVVVTEWNEFRRP
                     DFDRIKTTLKRPLVIDGRNLYDPKKMKSLGFEYDSIGRPQA"
BASE COUNT          630 a          812 c          760 g          633 t
ORIGIN      
        1 atcagatggc agcacggcga gaatcgcgtc tttgcccccc tgttcgcggg ccagcagcgc
       61 gccccagaga atagcgggag cagtgttgcg cgccaggggt tccgcgagaa cctggggagc
      121 cgtctgtgga aatatctcgc tcatctgtgc ctgaacctgc tcagcgtatt cctgtgaggt
      181 aatgacgaat tgtccggcag ccggtatgac aggcagaata cggcgacttg tgtcctgcag
      241 cagagtgcgg ttcccgacga gcgccagaag ctgtttgggc cacgattgtc gcgagagggg
      301 ccagagacgg gttccggaac cgcccgcgag aatcacggca aaaatgtttg aagatgtttt
      361 cttcatagaa gtcgctccag acaaggttat gaggttaccg atgttcgtac ccgcggcggc
      421 gatactccca ggcatgtcga atgatcacct gcagatccgc atactgcggt ttccagccca
      481 gcatcgactt cgccagcacg gcgttcccga ccagacgagg cggatcgcct tcacggcgcg
      541 cgcccaacac cacccgaatg ctggcaccgg taacccgttc cgcagtcgca atcacttcct
      601 tcaccgaata ccccgaacca ttgccgagat tcagcgctcc cgatcgtcgc tcgcgtcgca
      661 gatactcgag agccagcaca tgagcgtcgg cgagatcaga aacgtgaata tagtctcgaa
      721 tgcaggtacc gtctggggtc ggatagtccg tgccgaacac cgtgacatga ggacgttttc
      781 ccatggcggc gtcgagcacc aatgggatga gatgtgtctc gggatcgtgt cgctcaccaa
      841 tctcggcgtc aggatcgccc cctgccgcgt taaagtaccg cagtgccaga tagttcaacc
      901 catgagcgac gctgaaatcg gccatcattc gctccatcat cagtttcgag gcaccatacg
      961 gattgatggg atgctgcggg tgatcctccg gaatcggaat ggtacccgga atgccgtatg
     1021 tcgcacaggt cgaactgaat accagcatct gcaccccggt ttctcgcatg acttccagca
     1081 gatgcagact gcccagcaca ttgtttcggt agtattttcc cgggtcagag acagattcac
     1141 caacatacgt gaacgcggca aagtgcatca ccgccaccgg tcgatgtttt aggaaaactt
     1201 cgcgcaatcg agcctgatcg gcaagatctc cttcctcgag cgggccccag cggacaaatt
     1261 cacgatgtcc gtagatcaga ttgtcataga cgaccggctg atatccgctg agtgatagcg
     1321 ctttgcaggc atgtgagccg atatatccgg ctccaccggt caccaggacg accgggcgat
     1381 ccgtgttgtt gccgctcatt tcaacgatgc ggggtgctca gatcaatgac ggaattgtcc
     1441 ccgatgttga tacgctgggc gttaccggtc atctccgccg agtcaccgat gatcgagttg
     1501 tcgagaatga cattcttcag tgtcgaattg gcattgatga cactgtcgcg cacgatgacg
     1561 ttctcgagtt ggcaaccttc ggcaatcgac acgtgcggcc caatgaccga gtgatacacc
     1621 ctggcagaag ggtcgatcgc caccggagga atgacaacgc acccctcaat gcagtagtgg
     1681 ctgcgcgtcg agtgttccaa aagataccgg ttggtaccca gcagtgtttc gggtttgccg
     1741 caatcgagcc attcgtcgat ttcaggcgca ccgagtttca agccgtcttt cagcatcagc
     1801 ttgaacactt caggaaggta gtattcccct tttacggtca gcttctgatc gatcgcccga
     1861 gtcagataat ccatgaaccg cacgccatct ttcagatagt acagacccac ctgcgccaac
     1921 cgactgatcg gctgatcggg cttttccacc atgtcaacaa tgatgccgtc cttcatgacg
     1981 ttcacaccga accgccgata gtcttcaacc tctttggagt agatcaaacc atcgagaccg
     2041 tcgaccagac gcggaatgcg tgtcagatca gtgacgaaca gcgtgtcatt gaagaccacc
     2101 aacacatcgt cacccttttc gatacgcggt tttgcaagcc agaccgcatg agccgggcca
     2161 agcaactcgg actgcgtgat gtatgtggcc ttcgcttcgg gaaacttctg cttgacgaaa
     2221 ttctcgatga tatagccgtt ctcatcggta atgaagatgt actcaaccgc cttgagtttc
     2281 gagacgttct gcatcacgtg ttcgagaacc gttttaccgg caacccgcac cagcgatttc
     2341 ggcttggtgt gggtatgcgg gcgcaaacgg gttcctttgc ccgccaccgg aagaatgatc
     2401 ttcatgcctg aggcctccca atagaatcat attcaaaccc gagacttttc atttttttcg
     2461 ggtcgtacaa attgcgacca tcgatcacga gtggacgctt caaagtggtc ttgatccggt
     2521 cgaagtcagg gcgacgaaac tcattccatt cggtgacgac caccagagca tcggctccgt
     2581 ttaacgcatc gtacgagcgc ttggcatacg agatacgatc gccaaagacc tttttcgctt
     2641 cgtgcatcgc ttcggggtca taggcctgaa ccttggcacc agccttgaga agatgttcga
     2701 tgatgacgat ggcgggtgct tcgcgcatat catcggtgtt cggtttgaac gccaaacccc
     2761 agattgcgat ggtcttgccc ttgagatcgc ctttgaatgc cttgaccgcc ttgtgataca
     2821 gcaccatctt ctggc
//
LOCUS       DAORHD010000593         2812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41484_length_2834_cov_3.758546, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000593 DAORHD010000000
VERSION     DAORHD010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41484_length_2834_cov_3.758546"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="PKO06_12655"
     CDS             complement(<1..1493)
                     /locus_tag="PKO06_12655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70543.1"
                     /translation="MTKPTDPPDQEDLRRKILGLGPTSMRKSYYPELQKRLQELESSE
                     RRFRRIVEASPFGMLFFRQDVHRQWFCEDANGAAETIVCCSADRLRAVPLAQLPVDPA
                     LREALQAIVAQTGEPVATTEKSIDCQLGNTTKHLELAGFPIDPGHCGVYLYDSTNRLN
                     MEEQLRNAQRLEVVGRLAGGIAHDFNNLLTPILGYLQMVIEDTPAEDGRLEYLQEIKK
                     AAESARMISKSLLAISRRQILELTEIELVQTVRDFGKILRRTIRENVAICYELEEPPL
                     TIRADLTQMHQILLNLALNAQDAMLDGGTLTIRVFQQKAAHDCLLRGTYTQESGCVVL
                     QISDTGFGIPPDVLPRIFEPFYSTKGKRGSGLGLATVYGIVQQFGGQIRVRSSPGKGT
                     EFMIAFPGLNTKTECVQESTRSTTNRVERRSVYGQTVFLVEDDQQVRELVTVVLRDAG
                     YQIHAFPSAEDCLSWVPPETMKPAVLVSDVILPGRSGCDLYEDLKRR"
     gene            complement(1477..2631)
                     /locus_tag="PKO06_12660"
     CDS             complement(1477..2631)
                     /locus_tag="PKO06_12660"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV70544.1"
                     /translation="MEELRKFVAPEFLFGYGARLEAGHYAANYGISRALVVSDSGVIQ
                     AGWTGQVCDQLKQAGIASVVFAQVTPNPKDHEVHAGLEVYQKEDCNGVVAVGGGSVLD
                     CAKGIAILATNGRRIADYEGVDKIESPCPPLICVATTAGSAADVSQFAIITHTERRVK
                     MAIISKALVPDVALIDPETTFTMDPQLTAHTALDALTHAFEAGVSDASAPITDLLACS
                     AVRRVRRYLVNTLDQPRDHTARKELMQACLEAGLAFSNASLGAVHAMAHALGGVSNQA
                     HGECNALLLPYVVRRNLSASVPAYQRLAEAFGCQLEARASAEAVRDSLLPELQSFIAA
                     CKVKTRLRDLPLDRSLIPKLAAVAVADPCLITNPVKLSEEELAALYDEAY"
BASE COUNT          615 a          869 c          762 g          566 t
ORIGIN      
        1 cagcgccgtt tcaggtcctc gtagagatcg catccggaac gtccgggcaa aatgacgtcg
       61 ctgaccagaa ccgccggctt catcgtctcc ggcggaaccc agctcaaaca atcttcggca
      121 ctgggaaacg catggatctg atatccggca tcacgcagaa ccaccgtcac cagctcacgt
      181 acctgttgat catcttcgac gaggaacacg gtttgaccat ataccgatcg tcgttccacc
      241 ctgttcgtcg tggaccgggt gctctcctgc acgcactccg tcttggtatt caaccccgga
      301 aatgcgatca tgaattccgt tcccttgccc ggagaggacc gcacgcgaat ctggcccccg
      361 aactgctgaa cgatgccgta gaccgtggcg agaccaagac cagaaccacg ttttcccttg
      421 gtcgagtaaa acggctcgaa gatgcgcggc aacacgtcgg ggggaatacc gaaacccgta
      481 tcgctgatct gcagcacgac gcaacccgac tcttgcgtat aggtgccacg aagcagacag
      541 tcatgcgccg ccttctgctg aaacacccga atggtcagtg tcccgccgtc gagcatggca
      601 tcctgtgcat tcagagccag gttcaacaag atctgatgca tctgcgtcaa atcagccctg
      661 atggtcaggg gaggctcttc cagttcatag cagatggcaa cattttcgcg aatcgtccga
      721 cgcagaatct tgccgaaatc gcgcaccgtt tgtacgagtt caatttcagt caattcgaga
      781 atctgtctgc gactaatcgc cagcaaactc tttgagatca tacgggcaga ctccgcggct
      841 ttcttgattt cctgcagata ttccaggcgt ccgtcctccg cgggcgtgtc ttcgataacc
      901 atctgcagat agccaagaat cggtgtcagc aggttgttga aatcgtgagc gatgccccca
      961 gccaaacggc ccacgacttc gagacgctgc gcgtttcgca actgctcttc catgttcaga
     1021 cggttggtgg agtcatacag gtagaccccg caatgaccgg ggtcgatcgg aaaaccggca
     1081 agctcaagat gtttcgtcgt gttcccaagc tggcagtcaa tcgacttttc ggtggtggca
     1141 accggttcgc ctgtctgtgc gacgattgcc tgcaacgcct cacgcaacgc cggatcaacc
     1201 ggtaactgag ccaatggcac ggcccgaagc cgatcggcag agcagcagac gatggtctcg
     1261 gcggcaccgt tcgcatcttc acagaaccac tgccgatgca cgtcctggcg aaaaaacagc
     1321 atgccgaagg gagaggcttc gacaatccga cgaaagcgtc gctccgagct ctcgagttcc
     1381 tgcagacgtt tctgcagttc cgggtagtag ctcttgcgca tcgaggtcgg acccagacca
     1441 agaatcttgc ggcgcaagtc ctcttgatca ggggggtcag taggcttcgt catacagggc
     1501 agccagttcc tcctcagaca atttcaccgg attcgtgatc aggcacggat cagccaccgc
     1561 cacggcggcg agcttcggaa tcagcgaacg atcgagcggc agatcacgca gtctcgtctt
     1621 caccttacag gctgcaataa agctctgcaa ctcaggtaac agggaatcgc gcaccgcctc
     1681 agccgatgcc ctggcttcga gttgacagcc gaatgcctct gccagacgct gataggcagg
     1741 caccgaagca gaaagatttc gtcgcacgac atacggaaga agcagagcgt tgcactcgcc
     1801 atgcgcttga ttactgaccc cgccgagcgc gtgggccatc gcatgcacag cgccgagact
     1861 agcgttcgag aaggcgagac cggcttcgag acaagcttgc atcagttcct tgcgagccgt
     1921 gtgatcacgc ggctgatcga gcgtgttgac cagataccgc cgcacccgcc tgaccgccga
     1981 acaagccagc agatcggtaa tcggggccga tgcatcagag acaccggcct cgaaggcgtg
     2041 tgtgagcgcg tcaagggcgg tgtgagccgt caactgcgga tccatggtga aggtcgtctc
     2101 gggatcgatc agagccacgt caggcaccag agctttgctg atgatcgcca ttttgacccg
     2161 ccgttccgtg tgcgtaatga tggcaaactg cgaaacgtcg gcggccgaac cggcggtcgt
     2221 cgcgacgcag atcagtggcg ggcaagggga ctcgatctta tcgacgccct catagtcagc
     2281 aatgcgccga ccgttggtcg ccagaatggc tatgcccttg gcacaatcaa gcacgctacc
     2341 gccgccgaca gcaacgactc cgttgcaatc ttccttctga tacacctcga gacccgcgtg
     2401 cacctcatga tccttcgggt tcggtgtcac ctgcgcgaac acgacgctgg cgatgcctgc
     2461 ctgcttcagt tgatcacaca cctgaccggt ccagcccgcc tggatcaccc cactgtctga
     2521 cacgaccagc gctcgactga ttccgtagtt cgcggcataa tgccctgctt caaggcgagc
     2581 cccgtatccg aacaggaact cgggtgcaac aaacttgcgt agttcctcca tgatttccct
     2641 cccatacgct ctccgacttc cgtcgatccc aggctcccag tataccagaa ccgcccgcgc
     2701 tcctgcagat ccgacacgat cgtcagggtt tgcttatcac tcgcgagtgg tctatattcc
     2761 tgttgcccga tcgaagagaa tcgaatgatc gttctgggag gttccacatg tt
//
LOCUS       DAORHD010000594         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41487_length_2834_cov_3.543001, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000594 DAORHD010000000
VERSION     DAORHD010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41487_length_2834_cov_3.543001"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="PKO06_12665"
     CDS             <1..955
                     /locus_tag="PKO06_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70545.1"
                     /translation="GKLWAESTPGEGSTFQFILPFQAAPQTSVSVLDHKQPQLTDLKV
                     LIVDDNPTNCRILSLQTSKWGMLPRTASSGEQALAWLRAGEKFDFAILDMQMPGMDGE
                     TLGRAILADPKLASIRLIMLTSLGRRGDTRRFTEIGFKGFATKPIHHHTLKALLLQVL
                     ACPADCESGAAKTIVTGHSAWESYIPFQGSKVRILLAEDNPVNQQMALIMIQKIGLHA
                     DAVASGEEALKALEMIPYDLVLMDVQMPVMDGLEAARRIRDPNSRVRNHQIPIIAMTA
                     GAMLGDREKCLAAGMDDYLTKPVVKRSLIDILGKWLPRRPA"
     gene            complement(971..1516)
                     /locus_tag="PKO06_12670"
     CDS             complement(971..1516)
                     /locus_tag="PKO06_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70546.1"
                     /translation="MLSFKFHPMASMRLVKLVSIVLGCLILNGLTTASASAQANRKRI
                     VIITSPETTAIDCVSLDELKSIFAKRLFRVKGSEVIPVQRKPASELRKAFNRLVLKAD
                     EDQLKTYWIEEKLAGRESPPKTFDSINSVVAFLKKKPGAISYVCLDDLDEEKRREVQI
                     IPVKVGKDKVAPEDENYPLRY"
     gene            complement(1503..2783)
                     /locus_tag="PKO06_12675"
     CDS             complement(1503..2783)
                     /locus_tag="PKO06_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70547.1"
                     /translation="MKKTLLCAFLALTACIPPGYGASALKPEKWNETVEHQQARISEL
                     EQKLASLSQQMADLQKSLTSEMATLKSTSHPQSACGDVPESKTAVSNEACEQERQEIA
                     DLDNRLEEIERIGGRIHHTGWYDFVVRTNNKPGDKRHFDQAHLYLNFDSQLDRVWRAF
                     MELEIEHSPTANSGGVQGTIELDRGYIERRCGDLSKMFIGKFFVPLGLYTREHWAIST
                     DSIMKPIHDENMYVPNSPVGFMYQGALRQAAARSGRCEYSCFLTNGSEMYATNRTRDD
                     QLGGGADLRLNFGHDDRNRVGMTYYRQNNHAQQGRLEQNYLTFLDYYVQPRFSVRVEN
                     FTQNRDRRWNDVYVLYAGAKYQLSRQTYLHCSWNKGDDDKNGRAADQTIECLTLGWWP
                     KPYVRTKLEFSRHRFEDPSLATYNQLAGAIGVVF"
BASE COUNT          610 a          820 c          753 g          651 t
ORIGIN      
        1 gggcaagctg tgggcggaaa gcacgccggg cgaaggctcg accttccaat tcatcctgcc
       61 gttccaggcc gccccgcaaa ccagcgtctc cgtgctcgac cacaaacaac cgcaactcac
      121 cgacctcaag gttttgatcg tggacgacaa ccccacgaac tgtcgcatcc tctcgttgca
      181 aaccagcaag tggggcatgc taccgcgaac agcctccagc ggggaacagg cgttggcgtg
      241 gttgcgcgcc ggagaaaagt ttgatttcgc cattctcgat atgcagatgc cgggcatgga
      301 tggcgaaaca cttggacgtg cgattctggc tgatccgaag ctcgcctcga tacgtctgat
      361 catgctcaca tctctgggac gacggggtga cacccgccgg ttcaccgaga tcggcttcaa
      421 ggggttcgcg accaagccga ttcatcacca caccctgaaa gccttgttgt tacaggttct
      481 cgcctgcccc gctgactgcg aatcgggcgc ggccaagacg atcgtcaccg gtcattcagc
      541 ctgggagtcg tacattccgt ttcagggctc gaaggttcgc atcctgctcg cggaagacaa
      601 tccggtcaat caacagatgg ccctcatcat gatccagaag atcggcctgc acgccgacgc
      661 cgtggcttcg ggggaagaag cgctcaaagc cctcgagatg attccgtacg atctggttct
      721 gatggatgta cagatgcctg tgatggacgg tctggaagcc gcgcgtcgca ttcgtgaccc
      781 aaactcacgg gtccgcaatc atcagatacc catcatcgcc atgaccgccg gcgccatgct
      841 cggcgatcgc gaaaagtgcc tggccgcagg catggatgac tatctcacca aacccgtcgt
      901 caagcgttcg ctgatcgaca tcctgggaaa gtggctgcca cgccgacctg cgtaactgta
      961 ttcccgtggg tcagtaacgc agcggatagt tctcgtcctc gggggcaact ttgtccttgc
     1021 cgactttcac cggaataatc tgcacctctc ttcgtttctc ttcgtccaga tcatccagac
     1081 agacatagct gatggcaccg ggtttctttt tgagaaaagc aaccaccgaa ttgatcgagt
     1141 caaacgtctt gggtgggctt tctcgacccg ccagtttctc ttcgatccag taggttttga
     1201 gttgatcctc atcagccttc agtacgagac gattgaaagc tttcctcaat tcagaagcag
     1261 gtttacgctg cacgggaatg acttcactgc ctttcacgcg aaacagccgc ttggcgaaga
     1321 tcgacttcag ttcatcgagt gagacacaat caatggctgt cgtttcaggt gaggtgatga
     1381 tgacgattct tttgcgattc gcctgtgccg aagcactggc cgtcgtcaga ccgttcagaa
     1441 tcaagcagcc aagaacgatc gacacgagtt tcacaagacg catcgaggcc atggggtgga
     1501 atttaaaaga caacaccgat tgcccccgca agttgattgt aggtcgccag actcggatcc
     1561 tcgaaccgat gccgggaaaa ctcgagtttg gttcgcacat acggtttcgg ccaccagccg
     1621 agggtcagac attcgatcgt ctgatcggcg gcgcgaccat tcttgtcatc atcgccctta
     1681 ttccagctac aatgcaagta tgtctgacga ctcaactggt actttgcccc cgcatacaac
     1741 acgtaaacat cattccagcg gcggtcgcga ttctgcgtaa agttctcgac gcgcactgaa
     1801 aaccgcggtt gcacgtagta gtcgaggaaa gtcaggtagt tctgttccag acgtccctgt
     1861 tgagcgtgat tgttctggcg gtagtaggtc atgccgactc ggttgcggtc atcatgccca
     1921 aagttcaatc tcagatcagc accgccaccc agttgatcgt cacgagtacg attggtggca
     1981 tacatctctg aaccgttggt cagaaagcac gaatactcgc accggcccga ccgcgcagcc
     2041 gcctgtcgca aggccccctg atacatgaaa ccaacaggac tgttgggaac gtacatgttc
     2101 tcgtcatgga tcggcttcat gattgagtca gtactgatcg cccagtgttc gcgcgtgtag
     2161 agtccgagag gcacgaagaa cttcccgatg aacattttcg acaggtcgcc acaccgtctc
     2221 tcgatgtagc cgcgatcaag ctcgatggtc ccttgtactc caccactatt ggcggttggt
     2281 gagtgttcga tctcgagttc cataaacgct cgccagacgc ggtcgagttg agaatcaaag
     2341 ttcagataca gatgcgcctg atcgaaatgc ctcttgtctc ccggcttgtt gtttgtgcgc
     2401 acgacgaagt cataccagcc ggtgtggtga atgcggccgc caatacgttc gatctcttcg
     2461 aggcgattgt cgaggtcggc aatctcctga cgttcctgct cacacgcctc attcgagacg
     2521 gcggttttgc tttccggcac atccccgcag gcactctgtg ggtgcgatgt gctcttcagc
     2581 gtcgccatct cgctggtcag acttttctgc aggtcggcca tctgctgtga gagcgacgcg
     2641 agcttttgtt caagttcaga gattctcgcc tgctgatgct caaccgtctc gttccacttt
     2701 tccggtttca gtgccgaggc cccatacccg gggggaatgc aagcagtcag cgcaagaaac
     2761 gcgcaaagca gagttttttt catgccgtgt cacggtctcc tgtttaaagc ctgagaaacg
     2821 agtctcttat cggc
//
LOCUS       DAORHD010000595         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41505_length_2833_cov_3.862851, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000595 DAORHD010000000
VERSION     DAORHD010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41505_length_2833_cov_3.862851"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PKO06_12680"
     CDS             <1..347
                     /locus_tag="PKO06_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70548.1"
                     /translation="NKISTRIQARVSGTFALAQCMAYPNPARTFSRIRCSFTGANAVN
                     ITGQVTIVDVSGDEVTTLPLQSQGGGLYDAIWNLQNRQREIVGNGVYFAIIEAWGPDG
                     DIKERRKIAVLR"
     gene            362..988
                     /locus_tag="PKO06_12685"
     CDS             362..988
                     /locus_tag="PKO06_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70549.1"
                     /translation="MGLQPKVLLLICIICCLPAIQNCGSSGGGGTSDVIIADQLINDG
                     WANIQVGQYKTAVTNFQQALAQSMTDAQSVSANSGYGWALAKDGRINEAIPFFEVASG
                     KGDHEATVGLAGALIFRHQTSEDYLRSAELLDKLIKANNEAISIPHAGLGLNKAKIYA
                     LAAISFALANDLPNAKYYINKAAALDSTMVGTTVDRIDEAFTLLGWRD"
     gene            complement(1056..2567)
                     /locus_tag="PKO06_12690"
     CDS             complement(1056..2567)
                     /locus_tag="PKO06_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70550.1"
                     /translation="MKILSAIIDYIDDRLNPIVVKEFRQAVQGRFVSSVLILFLLVQL
                     AAIALTIIDRSYVHQSFQAGRDAFITLMVLLMITTMVIIPINSGIRIAGERSDINLDL
                     LFITTIKPSAIVRGKLFASFGLALLIYSACAPFMTFTYLLRGIDLPSIFLVLLVSLFA
                     VLVNLQIALFLGCLPASNIFKYLLLPVGLIFFFQNFGITMALVSDILRYGIGGRFNMM
                     DFFMGATCVLMLVFMMFTMTSTILTPPASNRAFPVRLSLTLSWLIIGVAVFLYSSMTG
                     YSGGIDAWAVMSIVALALSFLAAVSERDDPGIRVRRAIPEGRIKWLLSFLFYSGSANG
                     FAWSVVMIFLTVVGGFFASEMIGSGRFLNPYSGSDTFIEMMGFVAYCYCFALSSMLLQ
                     RTLLSNMFDRSVTWVIALFLAAIGGTVGPLVGYVFFQSDGYAWMLLNPTMIADRGHRD
                     LVALFLIPWTLISTLMALPWFSRQVAQFKPFNPETDRVRNRRVVAVFEDVLPT"
     gene            2615..>2833
                     /locus_tag="PKO06_12695"
     CDS             2615..>2833
                     /locus_tag="PKO06_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70551.1"
                     /translation="MRPLAEFGESEPAFDEIGQHIAAFGFIAIDRFNYEAAEAIRVQA
                     GMISQQSRDFLELLRLKLEIDELTLVHGS"
BASE COUNT          737 a          794 c          808 g          494 t
ORIGIN      
        1 ggaacaagat cagcacgcgc attcaagccc gcgtttctgg tactttcgct ctggcccagt
       61 gcatggcgta tcccaacccg gcccgcacct tcagtcgcat tcgttgttcc ttcaccggag
      121 ccaatgcggt gaatatcaca ggtcaggtga ccatcgtcga cgtctcaggt gacgaggtca
      181 cgacgctgcc cctgcaatca cagggaggtg gcttgtacga tgccatctgg aacctgcaga
      241 atcgtcagcg tgagattgtc gggaacggcg tctacttcgc catcattgaa gcctggggtc
      301 cggacgggga catcaaggaa cgccgcaaga tcgcggtgtt gcgataaaag gagtgcggaa
      361 tatgggactg caaccgaaag tcctgctgtt gatctgtatc atctgctgcc tgcccgcgat
      421 tcaaaactgc ggcagcagcg gcggcggcgg caccagcgac gtgatcatcg cggaccagtt
      481 gatcaacgac ggctgggcga atattcaggt cggacagtac aagaccgcgg tcacgaactt
      541 ccaacaggcg ctggcacagt ccatgactga tgcccagagc gtgagcgcca acagcggtta
      601 cggttgggcc ctggcgaagg acgggcgaat caacgaagcc attccgttct tcgaagtggc
      661 gagcggcaag ggagaccacg aggcgacggt cggtctggcc ggggccctga tctttcggca
      721 ccagacatct gaagactatc tgcgctcggc cgaactgctc gacaagctga tcaaagccaa
      781 taatgaagcc atctcgattc cgcatgccgg actgggcctg aacaaggcca agatctatgc
      841 gctggccgcg atctccttcg cgctggccaa cgatctgccg aatgccaagt attacatcaa
      901 caaggcggcg gccctcgact cgacgatggt cggcacaacg gttgaccgaa tcgacgaggc
      961 gttcaccctg ctgggatggc gtgattaagc gtcgacctga agaaacaaaa ggccccggaa
     1021 aaggggcctt ttttgttcgc cacttcagga caagatcagg tcggcagcac gtcttcaaac
     1081 accgccacga cgcgccggtt gcgcacccga tcagtttccg ggttgaacgg cttgaactgc
     1141 gccacctgac ggctgaacca cggcagagcc atcagcgtcg agatcagtgt ccagggaatg
     1201 agaaacagcg cgacgagatc gcggtggcca cgatcggcaa tcatggtcgg gttcaacagc
     1261 atccaggcat acccatcaga ctggaagaac acatagccga ccagaggccc aaccgtacca
     1321 ccgatcgccg cgagaaacag ggcaataacc cacgtgacac tgcgatcgaa catgttcgac
     1381 agcaacgtgc gctgcaacaa catggacgac agggcaaagc agtaacagta cgccacaaaa
     1441 cccatcatct cgatgaaggt gtctgaaccc gagtaggggt tcaagaatct tccggatccg
     1501 atcatctcac tggcaaagaa gccaccgacc acggtgagaa agatcatgac gaccgaccag
     1561 gcaaaaccat tggccgagcc gctgtaaaac aagaaggaga gcagccactt gatccgacct
     1621 tccggaatcg cccgcctaac ccgaatacca gggtcgtcac gctcgctgac cgccgccagg
     1681 aatgacaggg ccagagcgac aatactcatg acagcccacg catcgatccc accggaatag
     1741 ccggtcatcg acgagtagag aaagaccgca acgccgatga tcagccaact gagcgtcaac
     1801 gagagtcgta ccgggaacgc gcgattcgat gccggcgggg tcagaatggt gctggtcatg
     1861 gtgaacatca tgaagaccag catcaagaca caagtcgccc ccatgaaaaa atccatcatg
     1921 ttgaatcggc cgccgatgcc gtaacgcaag atgtcggata ccagagccat ggtgatgccg
     1981 aagttctgga agaagaagat cagaccgacc ggcagcaaca gatacttgaa gatgttcgat
     2041 gccggcagac agccaaggaa cagagcgatc tgcaggttga ccagaaccgc aaacaagctg
     2101 acgagcaaca cgagaaagat cgacggcaga tcgattccgc gcagcagata cgtgaacgtc
     2161 atgaatggcg cacacgcgct gtagatcagt aatgcgagac cgaacgacgc gaacagctta
     2221 ccgcgcacga tcgccgaagg cttgatggtg gtgataaaca gaagatcgag attgatgtcg
     2281 gaccgctcgc cagcgatgcg gattcccgaa tttatgggaa tgatgaccat ggtggtgatc
     2341 atcaacagga ccatcagggt gatgaaggcg tcacgcccgg cctgaaaact ctgatgcacg
     2401 tagctgcgat cgatgatggt cagagcgatt gccgccagtt gtaccagcag gaacaggatg
     2461 agaaccgacg acacgaaccg cccctgcacg gcctgacgga attccttgac cacgatggga
     2521 ttcaggcgat cgtcgatgta atcaatgatg gcagagagta tcttcatcac tgcacgtctc
     2581 ccttcgtcac gttcatgaac accgattcaa ggtcatgcgg ccgctggcgg aattcggcga
     2641 gtcggaaccc gcgtttgacg agatcggaca gcacatcgct gcctttggat tcatcgccat
     2701 cgatcgcttt aattatgaag ctgcagaagc aatccgggtt caggcaggca tgattagcca
     2761 gcaaagccgt gattttttgg agcttttgcg gttaaaacta gaaattgacg agctgaccct
     2821 ggttcacggc agt
//
LOCUS       DAORHD010000596         2832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41524_length_2832_cov_3.343536, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000596 DAORHD010000000
VERSION     DAORHD010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41524_length_2832_cov_3.343536"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="PKO06_12700"
     CDS             <1..393
                     /locus_tag="PKO06_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70552.1"
                     /translation="RRIERLLQIMDRTARRHAFLRDSLNRWQKEIQEVRQPLATRPRD
                     QRLQAQLRDTLVRFWDYTHLPYGHDVMIFAEINEEIERSTRLLRGTLSESHQLPVLKF
                     VPKKLGNLGPVVRDPFQFGVTSSGESGQ"
     gene            393..788
                     /locus_tag="PKO06_12705"
     CDS             393..788
                     /locus_tag="PKO06_12705"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV70553.1"
                     /translation="MRTIRWNRSRAGFTLIEMLVVLSILGVLITMVGTRNTIVLERSR
                     DASLMLQCQSYRNAIHQYVLETAGRFPDTLADLVPKHLPHVTTDWVGSRGSGKLHYDK
                     GTGAIALYNQQDTAPETGTDAKGKPYGEY"
     gene            789..1220
                     /locus_tag="PKO06_12710"
     CDS             789..1220
                     /locus_tag="PKO06_12710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70554.1"
                     /translation="MNANRFGRPGLTLIELLVCTVIIGILSMTALPLSKHFVRSQKED
                     LLKEALRDLREAIDRHYDKMQRRAPELPEEQKYPSRLQSLLESRSIRRIPLDPFTGRR
                     EWGTRSSTDPVNASYTDGMNIFDVYSLATATALDGRAYSDW"
     gene            1531..1878
                     /gene="cutA"
                     /locus_tag="PKO06_12715"
     CDS             1531..1878
                     /gene="cutA"
                     /locus_tag="PKO06_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNV70555.1"
                     /translation="MTDKRIVLVTVPDKKLGENIAAELIRLRLAACVNIIPGITSIYE
                     WEGKVETGSEILLIIKTRVEAFDALRRIVQALHPYQVPEIISVPVEMGLQKYLDWVDA
                     MVPPAGAAEKKQT"
     gene            1881..>2832
                     /locus_tag="PKO06_12720"
     CDS             1881..>2832
                     /locus_tag="PKO06_12720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNV70556.1"
                     /translation="MVRRYEVRPHTSTQFRIDYARELNSAQLEAVEHFQGPCLCIAGA
                     GTGKTRTLVYRVSRMIESGVAPESILLLTFTRKAAHEMLSRASDLGGASAMRVAGGTF
                     HSFASRTLREFAGNLGYRPDFSILDEGDSCDILGLIRAELGLNRKEVRFPRKETLRQI
                     LSKSVNCGLSIETLVERDYDHFAEHLTEMLKVFVQYDNYKKNHQMMDYDDLLIEMQTL
                     LEQYRPVREQLAERLRYVMVDEYQDTNRLQARITFLLGCAHRNVLVVGDDAQSIYSFR
                     GADYRNIHEFPTLFEGCRLVRLEENYRSTNHILDASNALME"
BASE COUNT          635 a          754 c          808 g          635 t
ORIGIN      
        1 cggcgcattg aacgtctgct gcagatcatg gatcgcacgg ctcgtcgcca tgcctttctg
       61 cgtgattcac tgaaccgctg gcagaaagag atacaagagg tgcgtcagcc cctggcgacg
      121 cgtccgcgtg atcagcggtt gcaggcacag ctgcgcgaca ctttggtccg tttctgggat
      181 tacacgcatc tgccgtacgg gcacgatgtc atgatttttg ccgagatcaa tgaggaaatc
      241 gaacggtcga cgcgtttact gcgtggaacc ttgagcgaat ctcatcaact gcccgtattg
      301 aagtttgtgc caaagaagct cggcaacctt ggaccggtgg tacgcgaccc gttccagttt
      361 ggggtgacgt caagcggaga atcaggccag tgatgaggac cattcgctgg aatcgatctc
      421 gagcgggatt caccctgatc gagatgttgg tggtcctttc gattctgggc gtcctcatca
      481 ccatggtcgg gacacgcaac accatcgttc tggagcgcag tcgtgatgcg tcgctgatgc
      541 ttcagtgcca gagctatcgc aatgcgattc atcagtatgt gctcgaaacg gccggacgct
      601 ttccggacac gctcgctgac ctggtaccga aacatctgcc gcacgtcacc acagactggg
      661 tcggaagtcg aggtagcgga aagcttcatt acgacaaagg aactggcgcg attgccttgt
      721 acaatcagca ggatactgct ccagagaccg gaaccgatgc caagggaaaa ccgtatggag
      781 agtattgaat gaacgccaat cgtttcggca gaccgggtct gactctgatc gagctgctgg
      841 tctgcactgt catcatcgga atcctgtcga tgactgcgtt gcctctatcg aagcattttg
      901 tccggtccca aaaggaagac ctgttgaaag aggcactgcg tgatctccga gaggcgatcg
      961 accgtcatta cgacaaaatg cagcggcgcg ccccagagct tccggaggaa cagaagtatc
     1021 cctcacgtct gcaatcgctg ttggaaagtc gttcaatccg tcgcattccg ctcgaccctt
     1081 ttaccggtcg ccgcgaatgg ggaacccggt cttcgacaga cccggtcaac gccagttaca
     1141 ccgatggaat gaacattttt gacgtgtact cacttgccac ggctacagcg cttgacggtc
     1201 gtgcatactc cgactggtaa agcggcaaag caacacacgt tcgattgccg caggccagcg
     1261 gggtgggaga gaagccgtgg acgatcagcc ttcgatcagg gaattcagcg tgagaaggat
     1321 ggtcaggacc gtggcgaaca ccagtgaaac attgatggcc ataacgttct cctcgatttg
     1381 attcctgatc actatcggca acgatgcgct gttcatgaag cctcggtttg acacgcgtca
     1441 attcgaccgg tacaatgaac tcgctgcccc acggggcatt atacgctttt cctctgaaaa
     1501 ttcaagcgat cgcagcgcga agggtgactc atgaccgata agcgaattgt cctggtgaca
     1561 gtcccggata agaagctcgg agagaacatt gcggctgaac tgattcggct gcggctggcc
     1621 gcgtgtgtga atatcattcc gggcatcact tccatctacg agtgggaagg aaaagtggaa
     1681 acgggttcag agatactgct gatcatcaag acccgggttg aggcttttga tgccttgcgg
     1741 cgaattgtcc aggctcttca cccctaccag gttccggaaa tcatttccgt gccggttgaa
     1801 atgggattgc agaagtatct cgactgggtc gatgcgatgg tcccaccagc gggtgctgct
     1861 gagaagaaac agacctgacg atggttcgcc gctatgaggt gcgcccacac acgtcgacac
     1921 agtttcgcat cgactacgcg cgcgaactga attccgcaca actggaggcc gtggagcact
     1981 ttcagggacc ctgcctttgc atcgccggtg ccggaacagg gaaaacgcgg actcttgtct
     2041 accgtgtctc acgcatgatt gaaagcgggg ttgcccccga atccattctg ttgctgactt
     2101 tcactcgcaa ggccgctcac gagatgctct cgcgcgccag cgatctgggt ggggcatcgg
     2161 cgatgcgggt tgctggcggt acctttcact cgttcgcgtc acgaaccctg cgggagtttg
     2221 cgggcaacct tggctaccgg ccggatttct cgattctgga cgaaggtgac tcgtgcgata
     2281 ttctgggtct gattcgtgcc gaactggggt tgaatcgcaa ggaagtccgg tttccccgca
     2341 aggaaaccct gcgtcaaatc ctctcgaaga gtgtcaactg cggattgtcg atcgagactc
     2401 tggtagagcg agactatgat cactttgccg agcacctcac ggaaatgttg aaggtgtttg
     2461 tccagtatga caactacaag aagaatcatc agatgatgga ttacgacgat ctgttgatcg
     2521 aaatgcagac ccttctcgaa cagtatcgtc cggtccgcga acaactggcg gagcggttgc
     2581 ggtacgtgat ggtcgacgag tatcaggata ccaaccggct gcaggcgcgc atcacctttc
     2641 tgcttggctg tgcgcatcgg aacgttctcg tcgtcggcga cgacgcgcaa agcatttatt
     2701 catttcgcgg tgctgattat cgcaacattc acgagtttcc aacgctgttt gaaggttgtc
     2761 gtctcgtgcg tctcgaagaa aactatcgca gcacgaatca cattctcgat gcctcgaatg
     2821 cgctgatgga aa
//
LOCUS       DAORHD010000597         2831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41546_length_2831_cov_3.339337, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000597 DAORHD010000000
VERSION     DAORHD010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41546_length_2831_cov_3.339337"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKO06_12725"
     CDS             <1..463
                     /locus_tag="PKO06_12725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV70557.1"
                     /translation="EKFDSYAATLAPHARIIRHDIVQGIEKENLLREAHFLLLPSRYP
                     NEGQPLVLIEGLAFGCVLIGTDYRALGDMIKPEQTGCFVETTPRSIATAIAHLADNPD
                     EYQQLSTGALQLYHEEFTPTRHIDRLLQVLHAAAIMPSVSATRAAHQQHDP"
     gene            453..914
                     /locus_tag="PKO06_12730"
     CDS             453..914
                     /locus_tag="PKO06_12730"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNV70558.1"
                     /translation="MTRERFEELFRRALKRIPEQYKRGVNTFFTDQRALRQKGTVGLY
                     ILGHYHTDWMSTGPAITLYYGSFRRVLRGCAERVYRREIAKTLAHELLHHWERQSGID
                     DLGEDDRLRLARWRRRLGQPTGDPTGRDFLEASLFLFVLFLLIGFMTALTQ"
     gene            1398..1667
                     /locus_tag="PKO06_12735"
     CDS             1398..1667
                     /locus_tag="PKO06_12735"
                     /inference="COORDINATES: protein motif:HMM:NF016028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNV70559.1"
                     /translation="MKRHVEEASPALTPADLEEMLTDAEYRLAQLESRYEQLANFVEQ
                     LNDVVVAQDRRIMGLERELAQQRENQQGSSGLGALSGIRSDKPTT"
     gene            1671..2132
                     /locus_tag="PKO06_12740"
     CDS             1671..2132
                     /locus_tag="PKO06_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HNV70560.1"
                     /translation="MKLATLCYLRREGKTLMLFRNKKPQDIHEGKWNGVGGKFEPGET
                     PETCARREILEECGLTAHTLVMKGFLTFPLFAKGEDWYVFVFLVTDFSGELITSAEGH
                     LEWIPNEKVLYLPLWPGDRHFLPFLDQPGLFSGRFEYVDGQLISHEMIVYP"
     gene            2382..>2831
                     /locus_tag="PKO06_12745"
     CDS             2382..>2831
                     /locus_tag="PKO06_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70561.1"
                     /translation="MGSTVVRSTEQIAPKTIINVTAQLTSAKKPEKTFDALLTSAQTP
                     AVTASPAAPDNASAADAAIVPETEEAKTEAGSLSLTTCPLTKPCLDSGNLAINISFFV
                     RLSGEFEQTGSGIQSLFHRAARLVSDIFREEQGWIGDPIGQFLNVSER"
BASE COUNT          624 a          836 c          762 g          609 t
ORIGIN      
        1 cgagaaattt gattcctatg cagctactct tgctccacat gcccgcatca ttcgtcatga
       61 catcgtacag ggcatcgaga aggaaaacct gctgcgtgaa gcacactttc tcctgcttcc
      121 ttcgcgttat ccgaacgagg gacagccttt agtactcatc gaaggactag cttttggctg
      181 tgttcttatc ggcaccgatt accgcgctct cggagacatg atcaagccag agcaaaccgg
      241 gtgcttcgtc gaaacgacac cacgcagcat cgcaacggcc atcgcgcacc tggctgacaa
      301 cccagacgag tatcagcaac tcagcacagg ggctctgcag ctgtatcacg aagagttcac
      361 cccgacaagg cacattgacc gcctgctgca ggtactgcac gccgccgcca ttatgccttc
      421 tgtttcggcc acacgagcag cgcatcaaca gcatgacccg tgagagattc gaggaactgt
      481 tccgcagagc gctgaagcgt attccggagc agtacaagcg cggggtaaac accttcttca
      541 ccgatcagcg agctctcaga cagaagggaa ccgtcggact ctacattctc ggtcactatc
      601 acactgactg gatgagcaca gggccagcga taaccttgta ctatgggtca tttcggcggg
      661 tgttacgcgg gtgcgccgaa cgggtgtatc ggcgtgagat agccaagacg ctggcgcacg
      721 aactcctgca tcattgggag cgacaatcag gcatcgacga tctgggcgaa gatgaccggc
      781 tgcgactggc tcgctggagg aggcgactgg gacagccgac gggcgacccg actgggcgcg
      841 actttctcga ggcatcgctg tttctgttcg tcctgtttct gctgatcggc ttcatgacgg
      901 ctctgacaca gtgacccttt ttctgtgtca gatgcctggg ttgagcatcc tcccacctcc
      961 ccggagccag tgcagacggc aacttttttc tcgagtagcg ggcccttcga cttcgggtcg
     1021 tcagaccgct gcgtgaactt tgagtgtcga tcaatcattt cgatcgatgc ctccggttct
     1081 cttcaagagg ctgggcactc ccgccgctcc tgacggcact ctcagtgacg tccatggtgg
     1141 atgtcgaatg gggtcatcga ccctctcgcc tggggcactc gggcacgacg cgcttgtcgt
     1201 cttatggggg cccagaaggc ctctgcgtca gcgaatggtt cacgtatacc ctgccgggac
     1261 cctcaaacaa ctgtcggttc ttcgggtctg tcctggttct gtgcttgatg attgggtgtc
     1321 gcgcaggtat gatgaagctg gtctgaagcc tttcccgcat ccggcttctg gcacgttcac
     1381 tcacggagaa ccccccaatg aaacggcatg tggaagaagc ttcgccggca ctgactcccg
     1441 ctgatcttga agaaatgctg accgatgccg agtatcgact ggcccaactg gaaagtcggt
     1501 atgaacaact ggcaaacttc gtcgaacagc tgaatgacgt cgtcgttgcc caggaccggc
     1561 gcatcatggg actcgagcgc gaactggctc agcaacgaga gaaccagcag ggttcctcgg
     1621 ggctcggagc gctcagtggc attcgtagcg acaaacccac gacctgagcc atgaaactcg
     1681 ccaccctctg ttatcttcgc cgtgaaggca agacgctcat gctgttccgc aacaagaaac
     1741 cccaggacat tcatgaaggc aagtggaatg gcgttggcgg caaattcgag ccgggtgaga
     1801 ctcccgagac gtgcgcccgc cgcgagattc ttgaagaatg cggtctcacc gcccatacgc
     1861 tggtgatgaa ggggtttctc acgtttcctc tcttcgccaa gggagaagac tggtatgtct
     1921 tcgtctttct ggtcaccgac ttctcaggtg agctgattac ctctgccgag gggcacctgg
     1981 aatggatacc gaatgagaag gttctctatc tgcctctctg gcccggcgat cgccacttcc
     2041 tgccctttct cgatcaaccc ggcctctttt cggggcgatt cgagtatgtc gatggccagc
     2101 tgatatcgca cgagatgatc gtctatccct gattgcctgc gctcttcgac gcacacccgc
     2161 ccgactcacg cgcctccgat gcattccttc tcgtctttga ggaatcgcat tcggtgtttc
     2221 cggcgattca taccacgaga aacatcatca caagcggctt ttgcagatcg caacattttt
     2281 tcaaaaaaaa gctaaagtct ggctgatttc atgacgatag aaataataga gctaagggaa
     2341 aacagatagc ccgccttata caaagaaata aggaggccgg tatgggaagc acggttgtcc
     2401 gcagcacaga acagatcgca cccaaaacca tcatcaatgt tacggctcag ctgacatcgg
     2461 caaagaaacc agagaaaaca ttcgatgctc tgctgaccag cgcacaaact cctgccgtca
     2521 ccgcatcacc tgctgcacca gacaacgcat cagccgcgga cgcggcaatc gtgcccgaaa
     2581 ccgaggaggc aaagaccgag gcgggctccc tttctctgac aacctgtccg ctcacgaaac
     2641 cctgtctcga ctccgggaat ctggccatca acatctcgtt ctttgtgcgc ctctccggag
     2701 aattcgagca gaccggcagc ggcatccaat ccctctttca ccgggcagcg cgcctggttt
     2761 ccgatatctt ccgtgaggag cagggctgga tcggcgatcc gatcggacag tttctgaacg
     2821 tcagtgaaag g
//
LOCUS       DAORHD010000598         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41581_length_2830_cov_3.219099, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000598 DAORHD010000000
VERSION     DAORHD010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41581_length_2830_cov_3.219099"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKO06_12750"
     CDS             complement(<1..737)
                     /locus_tag="PKO06_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70562.1"
                     /translation="MKSYRIIRALQVLVLVLVLLSGSSAFATDTGKFAFYYQRIEKVE
                     KILPDEKPTGFWDSTKYWLKKGWNAVRSTVEKTITRTVFPGEGSKLWYSEKDPTIAYR
                     MKRIKVESEQHLLQLMGVMDGDESKLSEGQKSLLYMYRNAQNQQLRELSKKAIYPRIN
                     VHLTDTTGFDNSSEFPHVTDDFWPKSMGFAIVMNSKRYSSPGDEKDAMSTLVHETAHC
                     TNLTLPELFGQYGPDKKHYANEIIKPRS"
     gene            complement(940..2094)
                     /locus_tag="PKO06_12755"
     CDS             complement(940..2094)
                     /locus_tag="PKO06_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNV70563.1"
                     /translation="MKKIADTVPAIEPDYRQFIEELKARITVARVTAARRVNRELITL
                     YWDIGRAIVDKQKTLGWGESVVDMVAVELKRSFPGISGFSPRNVRDMKRLYLTYHDGS
                     FWRQVVAKFARQVGECESSRQRGRAWSEDSTRDFLRQLVTEVPWGQNLLILNKLAEPA
                     ERLFYLRAVAQFGWSRNVLLNQIKAGAYERAAVAKKTHNFPLALPDHLAEQADEMMKS
                     SYNLEFLDIRRPVRERELEDRLIARLQTFLLELGYGFCFVGRQHRLTVGSKEYVIDLL
                     FYHRFLKALVAFELKVGPFEPEFAGKMDFYLNLLNDKERGPEDQPSIGIILCAEKDDV
                     EVEYALRTKANPIGVAVYELQSKLPGPLKGKLPTAKQLAAAIREELESGK"
     gene            complement(2091..2330)
                     /locus_tag="PKO06_12760"
     CDS             complement(2091..2330)
                     /locus_tag="PKO06_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70564.1"
                     /translation="MALASCKPVSEESSLKREIATSIFRLAEAGSGGKPILGYEILPN
                     KYFYKYQPPTPTKDLLAKFWELEKEAEKLLAGLAE"
     gene            complement(2554..2751)
                     /locus_tag="PKO06_12765"
     CDS             complement(2554..2751)
                     /locus_tag="PKO06_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70565.1"
                     /translation="MDNLLTIFERYIYVAIVVQTLSVSFIVFDSRGPTETFHRLLWAL
                     CAFFTGPLALIVYAWRGRKPA"
BASE COUNT          617 a          747 c          789 g          677 t
ORIGIN      
        1 gatcgcggct tgataatctc gttcgcgtag tgctttttgt ccggaccata ctggccgaac
       61 agttcgggaa gggtcaggtt cgtgcagtgc gcggtctcat gcacgagagt cgacatggcg
      121 tccttttcat cgcccggcga cgagtaccgt ttcgaattca tcacgatcgc gaaacccatc
      181 gacttgggcc agaaatcatc ggtgacatga ggaaattccg agctgttgtc gaacccggtc
      241 gtatcggtca gatgcacatt gatgcgcgga tagatcgctt tcttcgacag ctctctcaac
      301 tgctgattct gagcattgcg atacatgtac aacagcgatt tctgaccctc agacagtttg
      361 ctttcatcgc catccatgac gcccatgagt tgcaatagat gctgttcact ctcaaccttg
      421 atgcgcttca tgcgatacgc gatcgtcggg tccttttcgc tgtaccaaag cttgcttcct
      481 tctcccggaa aaaccgtacg ggtgatcgtc ttctcaacgg tactgcgcac ggcgttccag
      541 cctttcttca gccaatactt ggtggagtcc cagaagccgg tcggcttttc atcagggaga
      601 attttttcga ccttctcgat gcgctggtag tagaacgcga actttccggt gtcggtggcg
      661 aacgccgaac tgccggaaag cagcaccagc acgagaacca gcacctgaag agcacgaatg
      721 atgcgatagc ttttcatagc ggcccccatc gagggtattt ctttcattgt acacgccgtg
      781 aaaaaatccg gaagaggtcg aatcacgccc tttatctaaa gagaagtgtt acacatgcgg
      841 cagaagatcg gcaggggtcg tgatttttgc gcgttcaggc ttgatgcggc ggcgggcgaa
      901 ctcgatcgcc gcgagaatca acgtcgcttg gcatttcgtt catttccccg actccaattc
      961 ctcgcggatt gcggcggcga gttgtttggc ggtggggagt ttgcctttca acgggccggg
     1021 gagtttcgac tggagttcat agacggcgac gccgatgggg ttggctttgg tccgcagggc
     1081 atactcgact tcgacatcgt ccttttcggc acagaggata atgccgatgg agggctggtc
     1141 ttcggggcct cgttccttgt cgttgagcaa gttaaggtag aagtccatct tgccagcgaa
     1201 ctcgggttcg aacgggccga ccttgagttc aaaggcgacg agtgctttca gaaatcggtg
     1261 gtaaaagagc agatcgataa cgtattcctt gctgccgacg gtcaggcgat gctggcgacc
     1321 gacgaaacag aatccatacc cgagttcgag caggaatgtc tgcaaacggg caatgagacg
     1381 gtcttccagc tcgcgctccc tgaccggtcg acgaatgtcg agaaactcga ggttgtacga
     1441 actcttcatc atctcatcgg cctgctctgc cagatgatcg ggcagggcta gcggaaagtt
     1501 gtgcgtcttc ttcgccacgg cggcgcgttc gtaggcaccg gccttgatct gattgagcaa
     1561 tacatttctc gaccagccga attgcgcgac ggcacgcaga tagaaaagtc tttcggcagg
     1621 ctcggccagc ttgttgagta tcagcaggtt ctgcccccaa ggcacttccg tgacaagctg
     1681 tcgcagaaaa tctcgtgtgg aatcctcaga ccacgccctg cctcgttgcc ggctcgattc
     1741 acattcaccg acctgacgcg caaatttggc gacaacctgt cgccaaaatg atccatcgtg
     1801 gtaggtgaga tacagtcgct tcatatcgcg tacgttgcgc ggtgaaaagc cgctgatgcc
     1861 ggggaaagat cgtttcagtt ccaccgcgac catgtcgaca accgattcgc cccagcccaa
     1921 tgttttttgc ttgtcgacga tcgcgcgacc gatatcccaa tagagcgtaa tcagctcacg
     1981 gttgacccga cgggcggcag tgacacgagc tacggtgatt cgtgctttca gttcctcgat
     2041 gaactgccga tagtcgggct cgatggctgg tacagtatcg gcaatctttt tcattcagcc
     2101 agccccgcca gtaacttctc ggcttccttt tcaagctccc agaactttgc cagcaaatcc
     2161 tttgtgggtg tcggcggctg gtacttatag aagtatttgt tcggcagaat ctcatagccg
     2221 agaatcggct ttccgccact ccctgcctct gcgagacgaa atatcgatgt ggcgatctcg
     2281 cgtttcagag atgattcttc ggatactggc ttgcagctag caagagccat tccatctcct
     2341 gttttcggtc agggttcaca cggataatag agcattggca cacgcattca ttctatatcg
     2401 cagccgtttt ggcgacaagt tcttcttgtt tgaataacag gcaaatcaga ccgcaaaaaa
     2461 atgaagacgg gcgcgcatga tgtccagctt tcgaattgaa gacgtctgga agagcctgtt
     2521 gctgttggtg ttgacgtgcg agagaaagcg acgtcaggcg ggtttgcgac cgcgccaggc
     2581 gtagacgatc agcgcgaggg ggccggtgaa gaatgcacac aaggcccata acaggcggtg
     2641 gaatgtctcg gtgggcccac gcgagtcgaa cacgatgaaa ctgacgctga gtgtctgaac
     2701 gacgatcgcg acgtagatgt accgctcaaa gatggtcagg agattgtcca tgatgttggt
     2761 tcagaggcat tccgagtcag tcatgacgcg cttgtccacc agatgcgctg tcgtgctaga
     2821 ggctcagaat
//
LOCUS       DAORHD010000599         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41620_length_2829_cov_2.760995, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000599 DAORHD010000000
VERSION     DAORHD010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41620_length_2829_cov_2.760995"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKO06_12770"
     CDS             <1..348
                     /locus_tag="PKO06_12770"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV70566.1"
                     /translation="TILHFRWVRRGNYRDKEEWWTPRVSYRYEVNGRTYTSNTISGYD
                     TSELTTHEYWDFSRRAENCRVVIHYNPEKPQESVVMPFRSHFTVWTHLIAGVFVGGIG
                     GFVYLASYAPVRR"
     gene            complement(380..2035)
                     /locus_tag="PKO06_12775"
     CDS             complement(380..2035)
                     /locus_tag="PKO06_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV70567.1"
                     /translation="MKMKRIGDALIDAGVITNAQLQEALAYVKKRGIRLGEALIEMGL
                     IGENDLMHCLSEQLGIPSIPDEDLVVDPSVIHEVPYVMAKKHNLIPLGVQNGRLVVAT
                     FDPLNINIIDDIESRARRPVTLVLASSRRIAEAIEQYYSGAENIAQTLKQVQDMEDEN
                     ILARLDDEALSVQDTPVVKFVNQILQKAVKENASDVHIEVDHIDFHIRFRIDGLLQTM
                     FRPKMHLHSLIVSRLKVMSRMDVSEHRLPQDGRIMLKVENELVDFRTSTIPCMHGENM
                     VVRILNRGPQFRKLSELGFSDEGLKRLDGLVARSHGLVLVAGQTGSGKTTTLYSVLKL
                     LNDEKVKIITLEDPVEMQLAMLNQIQVNPKIDLTFANGLRSILRMDPDIIMIGEIRDR
                     ETAQLSINAAMTGHLVFSTIHTGTAAEVPVRLREMGVEPYLIANTLVGSIAQRLVRLN
                     CPNCLEEEQLPGRILGRFKPDFKSFHGKGCPSCNRIGYRGRTCIEEVMPTDTILRQKM
                     LAGASTEELEDAAVKAGMRTLFATGVDKVKAKLTSFEELARVM"
     gene            complement(2120..2584)
                     /locus_tag="PKO06_12780"
     CDS             complement(2120..2584)
                     /locus_tag="PKO06_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70568.1"
                     /translation="MLCILLLFSTTPAYAQPMKGLFLFLALLGALPFILTGVKSVYCA
                     LQERPMTFMEHLWILAFDALTVVLGIALLIREGGPAQSLATALVNLALSVCLAFTLYV
                     AYRYVIFGREEADVAYDRSSRFALLNSFLFLVVTTVRGVLVLAMELLEFLTP"
     gene            complement(2705..>2829)
                     /locus_tag="PKO06_12785"
     CDS             complement(2705..>2829)
                     /locus_tag="PKO06_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HNV70569.1"
                     /translation="TIGEGAVIGARAVVTKNMPAWQVCAGHPCQPLKPRVLRKT"
BASE COUNT          607 a          791 c          834 g          597 t
ORIGIN      
        1 accattcttc attttcgctg ggtgagaaga gggaactatc gcgacaagga agaatggtgg
       61 acgccacgcg tttcgtatcg gtatgaggtc aacggcagaa cctacacctc caacacgatc
      121 agcgggtatg acacctcaga gcttaccacg cacgaatact gggatttcag ccgtcgcgca
      181 gagaactgcc gggtcgtgat tcactacaac cccgagaagc cacaggaatc ggtcgtcatg
      241 ccgttccgca gtcattttac cgtgtggacg cacctgatcg cgggggtttt cgtcgggggg
      301 atcggcggat tcgtgtatct cgcatcgtat gccccggttc ggcggtagtc acggtagtca
      361 ccaatgaccg ttcgtgcggt tacatgacgc gggcgagttc ctcgaatgac gtcagtttgg
      421 ctttgacctt gtcgacaccg gttgcgaaca gggtgcgcat accggctttg acagcggcgt
      481 cctcgagttc ctcggtcgat gcgccggcca gcatcttctg ccgcagaatg gtgtcggtgg
      541 gcatcacctc ttcaatgcag gtgcggccgc gatagccgat gcgattgcac gacgggcagc
      601 ccttgccatg gaagctcttg aaatcgggtt tgaaccgacc cagaattcgc cccggaagct
      661 gttcttcttc gagacagttg ggacaattca aacgcaccag gcgttgcgcg atggaaccga
      721 caagggtgtt ggcgatgagg tagggttcga cccccatttc gcgcaggcgc acgggaacct
      781 cggccgcggt gccggtgtgg atcgtcgaga agaccagatg cccggtcatg gcggcgttga
      841 tcgaaagctg ggcggtttcg cggtcgcgaa tttcgccgat catgatgata tctggatcca
      901 tgcgtaaaat cgagcgcaga ccgttggcaa aggtcaggtc gatcttcggg ttcacctgaa
      961 tctggttcag catggccagc tgcatttcga ccgggtcttc gagggtgatg atcttgactt
     1021 tctcatcgtt gagcagcttg agaaccgagt agagggtcgt ggtttttccc gagccggtct
     1081 gaccggcgac gagcacgagg ccgtgcgagc gggcaaccag gccatcgagg cgtttgaggc
     1141 cctcatccga gaaacctaac tcggacaatt tccggaactg cggcccgcga ttgagaatgc
     1201 gtacgaccat gttttccccg tgcatgcagg gaatggtcga ggtgcggaag tcgaccagct
     1261 cgttttcgac cttgagcatg atacggccgt cctgcggcag gcgatgctcg ctgacatcca
     1321 tgcgtgacat gaccttgagg cgcgatacga tgagactgtg caggtgcatc ttgggtcgga
     1381 acatcgtctg cagcagacca tcgatgcgga accggatatg gaaatcgatg tgatcgacct
     1441 cgatatgcac gtcagaggcg ttttccttga cggctttctg caggatctgg ttcacgaact
     1501 tgacgacggg ggtatcctgc acgctgagcg cttcgtcgtc gagacgcgcc agaatgttct
     1561 cgtcctccat gtcctgaacc tgtttcaggg tctgcgcgat gttttcagcc ccgctgtaat
     1621 actgctcaat cgcttcagcg atgcgccggg aagaagccag caccagcgtc acgggacgac
     1681 gggcgcggct ttcaatatca tcgataatgt tgatattcag cgggtcgaag gtggcaacca
     1741 cgaggcggcc attctgcacg ccgagcggaa tcaggttgtg cttcttggcc atgacgtacg
     1801 gcacttcgtg aatgacgctg gggtcaacga ccagatcttc atcgggaatg ctggggatcc
     1861 cgagctgttc gctcaggcag tgcatcagat cattctctcc gatcagcccc atctcgatca
     1921 gggcttcacc gagcctgatc ccgcgcttct tcacatacgc aagcgcttcc tggagctggg
     1981 cgttggtaat cacgcccgca tcgatcaacg catcgccgat tcgcttcatc ttcatggtgt
     2041 gcccctcgct ggttcaggaa tctctgtcgc tggcaagctt accagaaaca atacccattt
     2101 gcctctgttt ttttcaaatc tagggagtca ggaactccaa caactccatc gcaaggacga
     2161 gaacgccacg aaccgtggtc acgaccagga acaggaacga gttcagcagg gcaaaccgag
     2221 agcttcgatc gtacgcgaca tccgcctctt cgcgaccaaa aatcacgtat cgatacgcca
     2281 catacagagt gaacgccaaa cagactgaca aggcgagatt caccagcgct gtcgccaggg
     2341 actgagcggg gcccccctcc cgaatcagaa gggcaatacc aagcacgacg gtcagggcat
     2401 cgaatgccag aatccacaga tgttccataa atgtcatggg ccgttcctga agtgcgcagt
     2461 acacggactt caccccggtc agaatgaacg gcaaggcacc tagcagtgcc aggaagagaa
     2521 acaacccctt catgggctgg gcatatgctg gtgtggtgga aaacagcaag aggatacaaa
     2581 gcacgcccgc ctgaagcagc gaacggtgat cacggtggtt tgcctcacct gcaactgatc
     2641 ggtgctgtct cttcatagtc ctgcccagga acgaaagcca cccgtggctc aggcggtcac
     2701 tcgttcacgt ttttctgaga acccggggtt tgaggggttg acacgggtga ccggcgcaga
     2761 cctgccaggc gggcatgttt ttggtcacga cggcgcgcgc gccaatgacg gctccttcac
     2821 cgatggtga
//
LOCUS       DAORHD010000600         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41686_length_2826_cov_3.542403, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000600 DAORHD010000000
VERSION     DAORHD010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41686_length_2826_cov_3.542403"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKO06_12790"
     CDS             complement(<1..278)
                     /locus_tag="PKO06_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNV70570.1"
                     /translation="MRFRLLNVGFGNVVVANRIVAIVSPGSAPMKRLKEEARESGKLI
                     DATYGRKTRAILVTDSDHIILSAINPETAALRFLGKKVADESEKATDV"
     gene            complement(288..1160)
                     /locus_tag="PKO06_12795"
     CDS             complement(288..1160)
                     /locus_tag="PKO06_12795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNV70571.1"
                     /translation="MVASLTGYGRAELLDESGRVCVECKSVNNRFLQLDLHIPYGYGW
                     LEERLRGLVGERVSRGKLIVNLEIFNFSSGQDIMINEPLLQRLVELQNKLALATGQRL
                     NLNLDGLLSLPGAMKVTASMDEERFWNRLKPVIEKALNTFSDARRREGENLARDMRGR
                     QKALLERLAVIEERLPTYREHFKTQFTNRLRELNEQAGLDESRLATEIALWVDRTDVT
                     EEITRLRSHLPELDSILSRKDSIGRRLDFLLQEIHRETNTLGNKLGDLAIIQQVLEMK
                     CEIEKIREQAQNLE"
     gene            1238..1987
                     /locus_tag="PKO06_12800"
     CDS             1238..1987
                     /locus_tag="PKO06_12800"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV70572.1"
                     /translation="MHWFDTHCHLDVEPLSEALPEVLARAHAAGVQQILVPAIHGPTR
                     LAEPIDGVRTAWGVHPGVAHQYQIEDLERLWHESAAPTSVAAIGEIGLDTMAESTPEQ
                     QEPQFIWQLQRAERHGLPVLVHLRGKWDHALRLLRQHAPDVPWIMHNFCGSWEIAHLY
                     LRAGAFLSFSGSLCRPGARKTPEVARRIPSERILVETDAPDLPPPAWTKTTNEPAALP
                     LIARFLAELRGEPIEALKETIWENSRRFFPL"
     gene            complement(2112..>2826)
                     /locus_tag="PKO06_12805"
     CDS             complement(2112..>2826)
                     /locus_tag="PKO06_12805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV70573.1"
                     /translation="FKSGILEGTLRERPASLRTGGQFLELDEIGILQLSENYLGQFQQ
                     RQLPPPLHQAVLHEDLPGVRLFLRGGTSPNQRDAKGNTPLHLCGLASRHEIAELLLAH
                     GADVQARNRDHRTPLHHAAEIGNASFAQLLLDHHADVNASDSYGITPTAWAISHGRQN
                     LALWLLEKGANSRVTNADGRSLAWLAVQYGQPHLLQQLIRLGIDLNQPDRQRKTPLMV
                     ARERRDETMIRLLTEAGAQ"
BASE COUNT          537 a          807 c          839 g          643 t
ORIGIN      
        1 tcgacgtcgg tcgccttttc ggactcgtcg gcgaccttct tgcccaaaaa ccgcagggca
       61 gcggtttcag ggttaatggc agagagaatg atgtgatcgg aatcagtgac cagaatggct
      121 cttgtcttgc gaccataggt ggcatcaatg agtttgccac tctcgcgagc ctcttccttc
      181 aagcgtttca tcggtgccga acctggactg acgatggcga cgatacggtt ggcgacgacc
      241 acgttgccga atccgacatt gagcagacga aagcgcatac cgttctccta ttccagattc
      301 tgggcctgct cgcggatctt ttcgatctcg cacttcatct cgagtacctg ctgaataatg
      361 gcgaggtcgc cgagcttgtt gcccagcgtg ttcgtctcgc gatggatctc ctgcaggaga
      421 aaatcgaggc gtcggccgat gctgtccttg cgtgacagga tgctgtcgag ttcgggcaga
      481 tggctgcgca gccgcgtgat ctcttcggtg acgtcggtgc ggtcgaccca cagcgcgatt
      541 tcggtggcca gacgcgactc gtcgagcccg gcctgttcgt tgagttcacg caagcggttg
      601 gtaaactggg ttttgaaatg ctcgcgatag gtcggcaggc gctcttcaat gaccgcgagg
      661 cgttcgagga gtgccttctg acgcccgcgc atgtcgcgag ccagattttc gccctcgcgt
      721 cgtcgcgcgt cggaaaaggt gttcaacgct ttctcgatga ccggcttgag acggttccag
      781 aagcgttctt catccatcga ggccgtgacc ttcatcgcgc ccggcaggct taagagccca
      841 tcgagattga gattcaaacg ctgcccggtg gcgagagcca gcttgttttg cagctcgacc
      901 agtcgctgca gcagaggctc gttgatcatg atgtcctggc cagagctgaa gttgaagatc
      961 tcgaggttga cgatcagctt gccgcgactg acgcgttcgc cgaccagccc tcgcaggcgt
     1021 tcttccagcc agccatagcc atagggaata tgcagatcga gttgcagaaa ccgattgttg
     1081 accgatttgc actcgacgca gactcgcccg ctttcgtcca ggagttccgc gcgaccatat
     1141 ccggtgagac ttgcaaccat agtcgccaat tataacccgt caagttgtct ttttcaatct
     1201 tcgaaaactg tgttacaaca atcttcagga acacacgatg cactggtttg atacgcactg
     1261 tcatctcgat gttgaaccgt tatctgaagc cctgccagag gtgctggcac gggctcacgc
     1321 tgcgggggta caacagattc tcgtgccggc gattcatgga cccacgcggc tggccgagcc
     1381 gattgacggt gtgagaaccg cctggggagt gcatccgggg gtggcgcatc agtatcagat
     1441 cgaggatctc gaacgactct ggcatgagtc agccgcgccg acctcggtcg cagcgattgg
     1501 cgagatcggt ctcgacacga tggccgaatc cacgcctgag cagcaggaac cacagttcat
     1561 ctggcagctg cagcgcgccg aacgacacgg gcttcccgtg ctcgtgcatc tgcgcggcaa
     1621 atgggatcac gcactcagac tgttgcgtca gcatgcgccc gacgtgccct ggatcatgca
     1681 caatttctgt ggctcctggg agatcgcaca tctctatctg cgtgctggag cttttctgtc
     1741 attttctggc tctctttgtc ggccgggggc gcgaaaaact cccgaggttg cccgccgcat
     1801 accgtccgag cgaattctgg tggaaaccga tgctcctgat ctcccacccc ctgcctggac
     1861 gaagacgaca aatgaaccgg cagcactccc gctcatcgcg aggtttctcg ccgagctgcg
     1921 tggagagcca atcgaagctc tgaaagaaac gatctgggag aactcccgcc ggtttttccc
     1981 actctgattg cctcatccgc tgttgttcat tcccggcgcg tgtgtgacca gcagttgtct
     2041 cacttacacc agctcatgtc tctgcattcc gatcagtcac gagaaccggt ttgccccatg
     2101 atgaacgggg gctattgtgc gcccgcttcg gtgagcagtc tgatcatggt ttcatcacgt
     2161 cgttctcggg cgaccatcaa aggcgttttc cgctgtcgat cgggttggtt gaggtcgatc
     2221 cccaaccgaa tgagctgttg caaaaggtgt ggttgtccat attgtaccgc cagccacgcc
     2281 agagacctgc cgtccgcatt ggtgacgcgt gagtttgctc ccttttcgag tagccagagg
     2341 gcaaggttct gtctgccgtg gctgatcgcc caggctgtcg gtgtgattcc gtacgagtcg
     2401 cttgcgttga catctgcatg atgatcaagc aggagctggg cgaaactcgc gtttccgatc
     2461 tcggcggcgt gatgcagagg ggtgcgatgg tcgcggtttc tggcttgcac gtcagcccca
     2521 tgagccagca acagctcggc gatctcatga cgcgatgcga gtccgcagag gtgcagaggc
     2581 gtgttgcctt tagcgtctcg ctggttcgga gaggtgccgc cacgcagaaa cagtcgaacg
     2641 ccggggagat cttcgtgtaa cacggcttga tgcaggggtg gcgggagctg gcgctgttga
     2701 aactgcccaa gatagttttc ggagagctgc agaatcccaa tttcgtcaag ttcgagaaac
     2761 tggcccccgg tgcgaagcga tgccgggcgc tctcgcaggg tgccttcgag gatcccgctc
     2821 ttgaac
//
LOCUS       DAORHD010000601         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41713_length_2825_cov_3.694585, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000601 DAORHD010000000
VERSION     DAORHD010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41713_length_2825_cov_3.694585"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1916)
                     /locus_tag="PKO06_12810"
     CDS             complement(<1..1916)
                     /locus_tag="PKO06_12810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HNV70574.1"
                     /translation="MIFSSLVVALQVSPLSADSLRQPVKPAEEVIRVHYQRSDGAYDR
                     WGLWVWDDVRTPSNPWPTGALRFSGRDDFGAWVDIELATAARKIGFLIVDRNTGDKES
                     GSKGFIMTSHSRHLYIRAGDDRVYTSSDLRVTPELRAATVIASHTVRVKCTDAQAITP
                     ASPAEAFTLKNVRGQELPITEAQVSGSDTVILTATFSLVDDVPLAVTFSGRTVSAPLS
                     WQLIDEMYGYDGDDLGCRIASGQAQLALWAPLATAVEVLLFDLRDQTRQIGRKPLNRT
                     NRGVWRITLSPADFPSGTATIEPFFYQFEVTNPGRPPKRVLDPYARSMAPVTVSPDGQ
                     SAGASGDFVGKAAMVAPETIGTAPTPSRIDGYQKREDAIIYEVHVRDFTSDPAIGSTL
                     KARWGSFSAFRERLTYIKSLGVTHVQLLPVMAWYFGDETRMNEREMHWSTRHNQYNWG
                     YDPQSYFSLDGAYSEKPEDPARRISEFKELIQAIHDAGMGVILDVVYTHMANGAFLDD
                     IVPDYYFFRDPQGNLLGDFGNNLATNRKMAAKLLVDSVQYWFRQYGIDGMRFDMMGDA
                     TADAIQRAFDAARAINPQAIFIGEGWRTFNGDAGAAAVTPADQDAMAQTDAVAVFSDE
                     VRNELKSGFGIEGQP"
     gene            2326..>2825
                     /locus_tag="PKO06_12815"
     CDS             2326..>2825
                     /locus_tag="PKO06_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HNV70575.1"
                     /translation="MISLIVHGGAWNIPDEAIEAHLAGVRQATEIGWQKLVAGGSAID
                     AVETAIRSMEDDPIFDAGRGSFLNAAGEIELDASLMDGTSFRAGAVAAVQNFRHPISL
                     ARLVMERSEHVLLVGEGAARFGRLHFMEPCAPEDLLVGRELERWRRLRQQPDFETPQA
                     FRADAPS"
BASE COUNT          547 a          807 c          910 g          561 t
ORIGIN      
        1 ggctggccct cgatgccaaa gccggacttg agctcgttgc gcacctcatc ggagaagacc
       61 gccaccgcgt ccgtctgcgc catggcgtcc tggtcggccg gggtgacggc agccgcgccc
      121 gcgtcgccgt tgaaggtgcg ccagccctcg ccgatgaaga tcgcctgcgg gttgatcgcc
      181 ctggccgcgt cgaaggcccg ctgaatcgcg tcggccgtcg cgtcgcccat catgtcgaag
      241 cgcatgccgt cgatgccgta ctggcggaac cagtactgca ccgagtcaac cagcagtttg
      301 gcggccatct tccggttggt ggcgaggttg ttgccgaagt cgcccagaag attgccctgc
      361 gggtcgcgga agaagtagta gtcgggcacg atgtcgtcga gaaatgcgcc gttggccatg
      421 tgcgtgtaga cgacgtcgag aatcacgccc atgccggcgt catgaatcgc ctgtatgagt
      481 tccttgaact cggaaatacg tcgcgccggg tcttcgggct tctcagaata cgcgccgtca
      541 agcgaaaaat acgactgcgg atcataaccc cagttgtact ggttgtgacg tgttgaccag
      601 tgcatctcgc gctcgttcat gcgtgtctcg tctccgaaat accaggccat gaccggcagg
      661 agctgaacgt gcgtgacgcc gagcgacttg atgtaggtca gccgttcgcg aaacgccgaa
      721 aagcttcccc agcgggcttt gagcgtggag ccgatcgccg gatcggaggt gaagtcgcgt
      781 acgtggacct cgtagatgat cgcgtcttca cgtttctgat aaccgtcgat tcgtgaagga
      841 gtcggagcgg tgccgatcgt ttcaggagcg accatggccg ccttgccgac gaagtcgccg
      901 cttgcgccgg ccgactgccc atcgggagag acggtgaccg gagccattga ccgggcatac
      961 gggtcgagca cccgcttcgg cggacgaccg gggttggtca cttcgaactg gtagaagaaa
     1021 ggttcgatag ttgcagttcc ggacgggaag tcagccggcg acagggtgat tcgccagaca
     1081 ccgcgattgg tgcgatttag cggcttgcgg ccgatctgtc tggtctgatc gcgaaggtcg
     1141 aacagcagca cctcgacggc ggtcgccagg ggtgcccaga gcgctagctg ggcctgtccg
     1201 gaggcgatgc ggcacccgag atcgtcgccg tcatatccgt acatttcgtc gatcagttgc
     1261 cacgaaagcg gggctgaaac cgtgcggccc gaaaaggtca ccgccagcgg aacatcatcg
     1321 accagcgaaa acgtcgcggt cagaatcacg gtatcacttc ccgagacttg cgcttcggtg
     1381 atcggcagtt cctgaccccg cacgtttttc agggtgaacg cctcggctgg cgaagcgggt
     1441 gtgatcgcct gagcatcggt acacttgacg cgaacggtat gactggcgat gacagtggcg
     1501 gctcgaagtt ccggagtcac ccgcagatcg gaggacgtgt aaacccggtc gtcgcctgcg
     1561 cgaatgtaca agtggcgtga gtgtgaggtc atgatgaatc ctttgcttcc agattccttg
     1621 tcgccggtgt tgcggtcgac gatgagaaag ccgatcttgc gggcggctgt ggccagctcg
     1681 atatccaccc aggcgccgaa gtcgtcccgc ccggagaatc gcagagcgcc ggtcggccac
     1741 gggttcgagg gcgttctgac gtcatcccag acccagagcc cccatcggtc atacgccccg
     1801 tccgaccgct ggtaatgaac ccggatgacc tcttcggccg gctttacagg ttggcgcaat
     1861 gaatctgcgc tcaatggcga aacctgcaga gcgacaacga gagaggagaa aatcactccg
     1921 agcactagcc tcagtaacct atggtgagag ttcactggtc cgtctcccgg tattctgatg
     1981 tttctggcag gtttttcgca atactcggcg tctcgaacgg aagctcggga ccggaattgc
     2041 gtcgaaatct gtttcttcag ggtagcagag gaacaccgct gcctcaaccc atgagtcaca
     2101 aagaaagggc agcatgcgaa ctccggtgct gcatgaaacc tgccctatga cacgtattca
     2161 cagtggtttt tagtaagaac ggtttcatga tagagtttaa cgaggcggac ccttggatct
     2221 ggtgatattg tcgttggtga caagtgatct gatcgtttct gtggcgacga actctccgac
     2281 agagtggctt gagaaagagg cagatactga cgtgaggagc atacggtgat ttcactgatc
     2341 gtccacggcg gagcctggaa catccccgat gaagccatcg aggcgcatct cgccggggtg
     2401 cggcaggcga cggagatcgg ctggcaaaag ctggtggccg gcggatccgc gatcgatgcc
     2461 gtcgaaaccg cgattcgcag catggaggat gatccgatct ttgacgcggg tcgtggttcc
     2521 tttctgaacg cagccggcga gatcgagctg gatgccagtc tcatggatgg aaccagtttc
     2581 cgcgccggtg ccgtcgcggc cgtgcagaac ttccggcatc cgatttccct ggcgcgactt
     2641 gtgatggaac gcagtgagca tgttctgctg gtgggcgagg gggcggcacg tttcggccgc
     2701 ctgcatttca tggaaccctg cgcacccgaa gatctgctgg tcgggcgcga actcgagcgc
     2761 tggcgtcgtc tgcggcaaca gcccgatttc gagacgccgc aggcgttccg cgccgatgcg
     2821 ccgtc
//
LOCUS       DAORHD010000602         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41815_length_2821_cov_2.441793, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000602 DAORHD010000000
VERSION     DAORHD010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41815_length_2821_cov_2.441793"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PKO06_12820"
     CDS             <1..1208
                     /locus_tag="PKO06_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNV70576.1"
                     /translation="ATASYYTYDTMLAQLQEWAAAYPQVTQVTSIGKSIEGREIWALK
                     VSDNPAVDELEPACLLVGGTHAREWIGVEVAMAALQAYLEGYSTDEKLTRLVNEREVW
                     FVPMLNPDGILFSQNSQRYWRKNRRPAGSSFGVDLNRNFGYKWGLTGASDKPYSDTYH
                     GAEPFSEPETRAVKELAERERFQTSLAFHSYSELILYPFAYGYNAPNPDEAVYKDIGG
                     QMAKFNKYKVQNCTELYPAMGISDDWLYGDQKTIALTFELAQEFIPPPQKIAAITAAT
                     VPAVLLMIEKTAAIAVNNPSGETAPTFSLDTTDGLEALAMGQRLMTQVSGFAREMLAA
                     RLQSTVRQVATVATDNALSGNSTSLTQVRQSPAAPLVMPQIRSRLRFEACHGRSIPSA
                     IMELVVPGR"
     gene            1335..>2821
                     /locus_tag="PKO06_12825"
     CDS             1335..>2821
                     /locus_tag="PKO06_12825"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNV70577.1"
                     /translation="MPRTRRLDSIVLFLSLCLACFTGSVAAQELQVWAMGAEGKLIRE
                     MADRFEAANPGVTVVTQAVSWGSAHEKLVTSIVGNMEPDVCQLGTTWMAEFHTMGALE
                     PIAPWLASSSIKLTEFFPASLDSNRFNGEYYGVPWYVDTRVFFYRTDLASAAGFARFP
                     ETWTELKTLAHNLKRLKSERGIPGFVYGPGIGDWQMLLMYFWQCGGEIQPDLWPGPII
                     DGESLREALEHIISFYDEGLSHREKGGEQELLAGTESGYYPMYVSGPWLVNDLESRPG
                     LKGRWTTAPLPRHRRGTSFLGGSNLVMFRGSKQKPLAWKFIEFMSSASNQALWYEISR
                     DLPARQSAWLLPALAERPHLEAFRRQLLDARAPPALPEWEAMAERLSHRYTEVMHGHR
                     GIAEGIALVQEECGLIIERSGIRPGVPFSWPLGLSLMLIPAFLAMLYLRTDPAQPPSP
                     RLRSRVWPFLLPALLLILAFRFFPGAACLVTSFTNWDIYGIQDPGR"
BASE COUNT          552 a          851 c          819 g          599 t
ORIGIN      
        1 cggcaacagc ttcctattac acctacgaca ccatgttggc ccaactgcag gaatgggccg
       61 ccgcatatcc gcaggtgaca caagtgacga gcatcggcaa gagcattgaa gggcgcgaga
      121 tctgggcgct gaaggtcagt gacaatcccg ccgtcgacga actcgaaccc gcctgtctgc
      181 tggtgggggg aacgcatgcc cgcgaatgga tcggtgttga agtggcgatg gcggctctgc
      241 aagcctatct tgaaggctac agcactgacg agaaactgac ccgtctggtg aacgagcgcg
      301 aagtctggtt tgtgcccatg ctcaaccctg acggaatcct gttttcacag aattcgcagc
      361 ggtactggcg caagaatcgc cgccccgccg gttcttcctt cggtgtcgac ctgaaccgga
      421 actttgggta caagtggggc ctgaccggag ccagcgacaa gccatacagc gacacctatc
      481 atggtgccga gcccttcagc gagccagaaa cccgggcggt caaggaactg gctgagcgcg
      541 aacgctttca aaccagcctg gcttttcata gctactctga actgattttg tacccgttcg
      601 catacggcta caacgcgcca aatcctgacg aagcggtcta caaagatatt ggcggacaga
      661 tggcgaaatt caataaatat aaggttcaaa actgtactga actgtaccct gccatgggca
      721 tttcagatga ctggttgtat ggcgatcaga aaaccattgc cctcactttc gaattggcgc
      781 aggagttcat tccaccgcca cagaagatcg ccgcgatcac ggcggccacc gtgccggccg
      841 tgctgctgat gatcgaaaaa acggccgcca tcgcagtcaa caatccatcg ggagagacgg
      901 caccgacatt ctcactcgac accaccgatg ggcttgaagc tctggccatg gggcaacgcc
      961 tcatgaccca ggtttccggc ttcgcccgcg agatgctcgc cgctcgcctg cagtcaaccg
     1021 ttcgacaggt ggcaaccgtt gcgacagaca acgctctttc tgggaacagc acttcgctga
     1081 cccaggtgcg ccagtcaccg gcagcccctc tggtgatgcc gcagattcgc tcgcgcctgc
     1141 gattcgaagc ctgccacggc cggtcgatcc cgtcagccat catggagctg gtcgttccgg
     1201 ggcgctgatc agccatccaa gcagggcggg gctcgtcggg ctccgccttt gttgtctctg
     1261 cgtgctgtcg aatggttgtg gtatgttcgt gacatgactg ttacatggcc cgaaaatcgt
     1321 gtgagatgac aggtatgccc aggactcgtc gactcgattc gatcgtgctg ttcttgtcgc
     1381 tgtgtcttgc atgtttcacg ggaagcgtcg cagctcagga gcttcaagtc tgggccatgg
     1441 gtgcagaagg caagctcatt cgcgagatgg ccgaccgttt cgaggccgcg aatcctggtg
     1501 ttaccgtggt cacccaggcg gtctcctggg gaagcgcgca tgaaaaactg gtcacctcga
     1561 tcgtgggcaa catggagccc gacgtctgcc agctgggcac gacctggatg gccgagtttc
     1621 acaccatggg tgctctcgaa cccatcgccc cctggctcgc ttcttccagc atcaagctga
     1681 ccgagttctt tccggcctca cttgactcga accgcttcaa tggcgagtat tacggagtcc
     1741 cgtggtatgt cgatacccgg gtgttcttct accgcaccga tctcgcttcg gccgcaggct
     1801 tcgcccgatt tcccgaaacc tggaccgagc tgaaaaccct ggcgcacaat ctcaaacgcc
     1861 tcaagtcgga acgcgggatt cccgggttcg tctacggccc cggaatcggt gactggcaga
     1921 tgctactgat gtatttctgg caatgcggtg gagagattca gcctgatctc tggccgggcc
     1981 ccatcatcga cggagaatcg ctgcgggaag cactggagca catcatctcg ttttacgacg
     2041 aagggctctc acatcgcgag aaaggcggcg aacaggagct gctggctggt acggaatccg
     2101 ggtattatcc catgtatgtc tcgggcccct ggctggtcaa cgacttggaa agccgccccg
     2161 gactgaaggg gcgatggacc acggcaccgc tgccgcggca tcgacgcggc acctcatttc
     2221 tcgggggttc aaatctggtc atgtttcgcg gctcgaaaca gaagccgctc gcctggaagt
     2281 tcatcgagtt catgagttcc gcctccaatc aggctctgtg gtacgagatt tctcgtgatc
     2341 tgcccgcgcg acagagcgcc tggctcctgc cggcccttgc tgaacggccg catctggaag
     2401 cctttcgacg tcaactgctc gacgcgcgtg cgccgccggc tctgccggag tgggaggcca
     2461 tggcagagcg cctgtcgcac cgatataccg aggtgatgca cggtcaccgg ggtatcgctg
     2521 agggaattgc gctggtgcag gaagagtgtg gattgatcat tgagcgcagt ggaattcggc
     2581 caggcgttcc gttttcctgg ccgctgggcc tgagtctgat gctgattccc gcctttctgg
     2641 ccatgctcta tctccgcacc gacccagccc aaccaccatc tccccgcctg cgttctcggg
     2701 tttggccctt tcttttgccg gctctgctgc tgattctcgc ctttcgtttt ttcccgggag
     2761 cggcctgtct ggtaaccagt ttcaccaact gggacatcta cggcattcaa gacccgggac
     2821 g
//
LOCUS       DAORHD010000603         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_41976_length_2813_cov_4.075417, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000603 DAORHD010000000
VERSION     DAORHD010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41976_length_2813_cov_4.075417"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2797)
                     /locus_tag="PKO06_12830"
     CDS             complement(<1..2797)
                     /locus_tag="PKO06_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70578.1"
                     /translation="MTVLASRPARLEVDIRDSDMIATAGETLTVWFWIKDAFGNPAST
                     TGTLTIRSTDTNPSASPTPVNQNLAAATSLYARNWILASAGVQFITASLTHPLGTIGE
                     TFSDPFLVTADTPDSITGDIPTDGTSELPTAFTIRVRDVYGNPTPLFTEGIRATSPGM
                     GFAQEQYYYSSDDKGEHTFLLRWDLGTDEPINTTVSFGHLRITTPPANALATLTRNIR
                     IDNPRDADGAPPTLPFMNLLLSMPDPPPIASVPFLMTLWSLSIRETPATLSTWIDFSA
                     NNGDVLVSTDGVNYGRSIFLNNAATANITAVVTRSGYITVTAKSRNEPTKGGTITFVS
                     RPNGLDTLTLTANSPQKARTRFPFKAEWWDVCRNYAELAQTSLTFNASAIGSFVYDPS
                     TVETGGRRGVFDPSANTQWVDTTFIASVSATNIDNDLILNRISLFPVYDNDFRKGIYN
                     TPWRSWQELAMVGDGYNEGGATFDGFLYLYHRGSGRIAPNDLDTRFNINDGYHFLYFN
                     WPAWDTYSFNADIFIRRLEYPPVQTDTDEHMLGVMARDDVDLANQRHRYICSQLFNTN
                     EPDNMGCRAQVSYRNAEDGDTRIRYLTGAAIDSWTSHPKWLRLTRNATALAWEAYMSP
                     DGELWRAMTSSGEAAPTYGWTVTKLGIAASSGLNTRRGGGWVTDFRINRFTATGSFVS
                     TVYDIGTQPTSLGPVAMTATFTGGATLAFDFKASDTVGPFADINNGWTPLALSAPSGN
                     SFTVTLPAGLHRRYMQYRLRLSAPELEAGRGIASVTPIVYDVNIPYTIANPLGVTFDQ
                     RGWVNLIASSASPAASYTLPVEIKGGVVATLAITVPASTTAGAPLTVTVRAGDGSAGV
                     FGNVADDATGTWRFTTSDGEPFPGTIPGDYTLVPAVDKGQRTFYNSSVLRSGMYQTIT
                     VTDGTRTATSSLILVNPG"
BASE COUNT          589 a          720 c          901 g          603 t
ORIGIN      
        1 ggccaggatt gacgaggatg agagatgaag tcgccgttct ggtgccgtca gtgaccgtga
       61 tcgtctggta cataccgctg cggagcactg atgaattgta aaatgttcgt tgtcccttat
      121 cgacggctgg aacgagcgta taatcgccgg gaatcgtccc ggggaatggt tcgccgtcgg
      181 aggtggtaaa acgccaggtt ccggttgcat catcggcgac gttgccgaac acgccggccg
      241 acccgtctcc ggcgcgcaca gtgacggtca gcggggctcc ggcggtggtg gaagcgggaa
      301 ccgtgatggc gagcgtcgcc actacgccgc ccttgatctc gaccggcaga gtataagagg
      361 cggccggaga tgcggaagac gcaatcagat tgacccaacc acgctggtcg aacgtgacgc
      421 cgagcgggtt ggcgatcgtg tacggaatgt tcacatcata aacgatgggt gtgactgaag
      481 cgattccgcg accagcctcc agttccggag cggacaggcg caggcgatac tgcatgtagc
      541 gtctgtgcag gccggccgga agagtcacgg taaagctgtt tccgctcgga gccgagaggg
      601 cgagaggggt ccagccattg ttgatgtcgg cgaatggacc gacggtgtca ctggccttga
      661 agtcaaaggc gagcgtcgca ccaccggtga aggtcgctgt catcgccact ggccccagtg
      721 aagtcggttg ggtgcctatg tcatacacgg ttgacacaaa agaaccggta gccgtgaaac
      781 gattgatacg aaaatcggtg acccatcccc caccacggcg cgtgttcaaa ccggaacttg
      841 ccgcgatccc aagtttggtg acagtccacc cataggttgg agctgcctcc ccggaactgg
      901 tcatcgcccg ccataactcc ccgtctggcg acatgtaagc ttcccacgct aaagcggtcg
      961 cattccttgt cagacgtaac cacttggggt gtgaagtcca tgagtcgatt gccgcccctg
     1021 tgaggtaacg aatacgcgta tcgccgtcct cggcatttcg atagctcacc tgcgcacggc
     1081 atcccatgtt gtcaggttca ttggtgttga acaattgcga acagatgtaa cggtggcgct
     1141 ggttggcaag gtcgacgtca tcacgcgcca tgactccgag catgtgctca tcagtgtccg
     1201 tctggaccgg aggatactcg aggcggcgga tgaaaatgtc tgcgttaaaa ctgtatgtgt
     1261 cccaggcggg ccagttgaaa tagagaaaat gatacccgtc gttgatgttg aaacgagtgt
     1321 cgagatcatt gggtgcgatt ctcccgcttc ctcgatggta caagtagaga aaaccgtcga
     1381 acgttgctcc accttcgttg tagccgtctc caaccattgc cagctcttgc caggaacgcc
     1441 agggagtatt gtagatgcct tttcggaagt cattgtcgta gacggggaac agactgatgc
     1501 gattgagaat caggtcgttg tcgatgttcg tcgcgctgac cgaagcgatg aaggtcgtgt
     1561 ccacccactg cgtgtttgcc gatggatcga agaccccgcg ccgaccgccg gtttctaccg
     1621 ttgagggatc atacacaaaa ctgccgattg ccgaggcgtt gaatgtgaga ctggtctgtg
     1681 ccagttccgc gtagttgcgg cacacgtccc accactccgc tttgaacgga aagcgagtgc
     1741 gtgctttttg cggcgagttg gcggtcaatg tgagcgtatc gaggccgttc gggcgtgaaa
     1801 cgaatgtaat cgtaccaccc ttggtgggtt cgttgcgact cttggcggtg acggtgatgt
     1861 atccggagcg ggtgacgact gccgtgatat tggctgtggc agcattattg aggaaaatgg
     1921 accgaccata gttgaccccg tctgtcgaga ccagaacatc gccgttgttg gcggaaaagt
     1981 cgatccaggt ggatagtgta gcgggtgttt cgcggatcga aagcgaccag agcgtcatta
     2041 gaaatggtac ggaagcgatc ggtggcgggt cgggcatact cagcagaaga ttcatgaagg
     2101 gcagagtcgg cggcgctccg tccgcgtcgc gcgggttgtc gatacgaatg ttgcgggtca
     2161 aggtcgccaa tgcattggcc ggcggtgtcg tgatgcgcag gtgcccgaaa gagaccgtcg
     2221 tgttgatggg ttcatcggtt ccaagatccc acctcaacag gaaggtgtgt tcgcccttat
     2281 cgtcgctcga atagtaatac tgttcctgag cgaagcccat gccgggtgag gtcgcgcgaa
     2341 tgccttccgt gaagaggggt gtcgggttgc cgtagacatc gcgaacacga atggtgaaag
     2401 cggttggaag ctcgctggtt ccgtccgttg gaatgtcgcc ggtgatactg tcgggtgtat
     2461 ctgcagtcac gagaaaagga tcactgaacg tttcgccgat ggttccgagc gggtgcgtca
     2521 gactcgcggt gataaactgt acccctgccg aggcgagaat ccagtttctg gcatagaggc
     2581 tggtggccgc ggccagattt tgattcaccg gagtgggaga tgccgacgga ttggtgtcag
     2641 tactccggat ggtcagggtg ccggtagtgc tcgcaggatt tccgaatgca tccttgatcc
     2701 agaaccagac cgtgagtgtc tcaccggcag tggcgatcat atctgagtcg cgaatgtcga
     2761 cttcgagtcg ggcaggtcgc gaggccagca ctgtcaccgt catggaaccg aga
//
LOCUS       DAORHD010000604         2806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42126_length_2806_cov_3.930571, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000604 DAORHD010000000
VERSION     DAORHD010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42126_length_2806_cov_3.930571"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="PKO06_12835"
     CDS             complement(<1..289)
                     /locus_tag="PKO06_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70579.1"
                     /translation="MKAEIPLSRGLYTDVVDIRRRVFAEIARIFWTADYRDIALIKDE
                     LVKIPYRVINREDPTYRCCVSRERAIVRERSRLAIGLPPWKGELSGPMYQGL"
     gene            complement(279..521)
                     /locus_tag="PKO06_12840"
     CDS             complement(279..521)
                     /locus_tag="PKO06_12840"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV70580.1"
                     /translation="MVEVTVCLGTDCAYRGGLQIHEYLEDDASLTEKIILRTARCLNR
                     SCKPDQAPIVIVNGKKIERATLDKVLVAIRENLHES"
     gene            complement(692..2239)
                     /locus_tag="PKO06_12845"
     CDS             complement(692..2239)
                     /locus_tag="PKO06_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70581.1"
                     /translation="MNWRRATLCLWLFSVWFTCYAGSVLANGPIDRLDRWFVFGTNLA
                     WFNNNYGADIGVNHAEGGWQPTFTRENAERVFANLAKMGCPVVRIWAFERQEGLLFTP
                     DLTSDPKYPYHEVTGLCPVFLRNCRTIMELAEKYRVKVYWSLLNHLIREEQGGRHLRI
                     ITETKVRNSYIRNAAVPFVKAMAASPAFFAVDIINEADGTIGKIDALSGCMNPLQGCT
                     WKEMRVFIKACAEAFHAAVPGIKVTATSGWHEEKNVKAGRFSGLGLDFYDWHSYRDVP
                     DLPHARTLGLDKPVIIGECGPKTQDPDAGYKLQWKNWWAYLAQARKGYAGLLTWSYGN
                     PGGATNFVMVEADHAWRAGAKMIYQATRGAHIPDAGPLMLTDDETRSLEAVNRAMQPI
                     LQLAGRSSLPASGEAQTRLKQLVKTTGMYYPYLNPKYAVVSFVRVSETLVAVIRHEQR
                     VAGSENLESLKQIGRDVLASIQTIEPLSQLQIVKTLTGLVSEVPSSESAGASPYDTDA
                     LILTPSR"
     gene            2534..>2806
                     /locus_tag="PKO06_12850"
     CDS             2534..>2806
                     /locus_tag="PKO06_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70582.1"
                     /translation="MRVPSVVGLRRSVRRLGFLSVPMIIILVVILFVAWQFFYWTFCR
                     IEIDPGMSGVLIAKMGTDLPPGEIIATQTGYKGIQFDVLSEGRHFYN"
BASE COUNT          587 a          842 c          786 g          591 t
ORIGIN      
        1 cgagcccctg atacatcggc ccgctcagtt cacccttcca gggcggcagg ccgatcgcca
       61 ggcgactgcg ttcgcgcacg atcgcccgtt cgcgcgacac gcaacagcga taggtcgggt
      121 cttcgcgatt gatcacccgg tacggaatct tcaccagctc atctttgatg agcgcgatgt
      181 cacgatagtc ggcggtccag aagatgcgcg cgatctcagc gaacacacgg cgccgaatat
      241 cgacgacgtc ggtatagagg cctcgtgaca gcggaatctc agctttcatg cagattctcg
      301 cgaatggcaa ccaggacttt atcgagcgtc gcgcgttcaa tcttttttcc gttcacgatc
      361 acgatcggag cctgatcagg cttgcacgaa cgattgagac agcgtgccgt gcgcagaatg
      421 attttttccg tcagcgacgc gtcgtcttcc agatactcgt gaatctgcaa ccccccgcga
      481 tacgcgcaat ccgtcccgag acacaccgtc acttccacca tggtcatcct cccgcattcc
      541 gcctgttgcc aactgcacga aggaaccgcg tgtcagacgc gatcattcgt cagcgagtcc
      601 ggcacccgca tgataccgcg aaacggtgaa aaagatcgag ccctgcgacc tcatgaagat
      661 cgcagggctc aggagacctc tcgcgggccg attaccgaga gggagtcagg atcagcgcat
      721 cggtgtcgta gggcgaagcc ccggccgatt ccgacgacgg aacttcagaa accaaaccag
      781 tcagggtttt cacgatctgc aactgagaca atggttcgat tgtctgaatc gatgcgagaa
      841 cgtcacgacc gatctgcttg aggctttcga gattctcgga accagcaact ctttgttcat
      901 ggcgaatgac ggcaacgagc gtctcggaca cccgcacgaa actcacgacc gcgtatttcg
      961 ggttcagata cggatagtac atgccggtcg tcttgaccaa ctgtttcaga cgagtctgtg
     1021 cttcaccgga agccggaagg gaggaacgac ccgccagttg cagaataggt tgcatggcgc
     1081 gatttacggc ctcgagcgaa cgggtttcat catcggtcag catcaggggg ccggcatcgg
     1141 gaatgtgcgc tccgcgggta gcctgataga tcatcttcgc gccggcacgc cacgcgtggt
     1201 cggcctcgac catgacgaag ttggtggcac cgccaggatt tccgtaggac caggtcagca
     1261 aaccggcgta gcccttgcgt gcctgagcga gataggccca ccagttcttc cactgaagct
     1321 tgtagcccgc gtccggatcc tgggttttcg gaccgcattc gccgatgatc accggcttgt
     1381 cgagccccag cgtgcgggcg tgcggcagat ccggaacgtc acggtacgag tgccagtcat
     1441 agaaatcgag ccccagaccg ctgaaacgac ctgctttgac attcttttcc tcgtgccagc
     1501 cgctggtggc ggtgactttg atgccgggga ccgccgcatg gaacgcctcg gcgcaggctt
     1561 tgatgaacac gcgcatttcc ttccaggtac agccctgcaa cgggttcatg cacccggaaa
     1621 gtgcatcgat tttgccgatc gtgccgtcgg cctcattgat gatgtcgacc gcgaagaagg
     1681 ccggcgatgc cgccatggcc ttcacgaacg gcaccgcggc attacgtatg tacgagttgc
     1741 gcactttcgt ttccgtgatg atacgcagat gacgaccacc ctgttcttcc cgaatcaggt
     1801 gattcagcag agaccagtac actttcacgc gatacttttc ggccaactcc atgatcgtgc
     1861 ggcagtttcg caggaagacg gggcagaggc cggtcacctc atggtacgga tacttcgggt
     1921 cggacgtcag atcgggagtg aacagcaggc cttcctgccg ttcgaacgcc cagatgcgca
     1981 cgacagggca ccccatcttc gcgagattcg cgaacacccg ttcggcgttt tcccgggtga
     2041 acgtgggctg ccagccgcct tcggcgtggt tgacgccgat atcagcgcca tagttgttgt
     2101 tgaaccaggc gagattggtg ccgaacacga accagcggtc gagccgatcg atcggcccgt
     2161 tcgccagcac cgatcctgca tagcaggtga accagaccga gaacaaccag agacacagcg
     2221 tcgcgcgacg ccaattcatg gccatttcca tgctctgctc ctttgccgga caataccatc
     2281 cgagccgaaa cctcggctta tttgtacaag tatagccacc aaagtgccca acccggaaga
     2341 cccgggcagt gatgtgttaa cggacaggaa tgcagtctga agccgattcg atattctccg
     2401 tggcttgtct cgtctgctgt cagcggcact ctccgaattc accggaagag gcatcttccg
     2461 cttctctctt gatggcgtga tatactgcga agataattgc cttcgagccc attccatccg
     2521 caggaggagc cgcgtgagag tcccgtccgt tgttggtctc cgacggtctg tccgtcgtct
     2581 cggttttcta tcggtaccga tgatcatcat tctggtcgtc attctgttcg ttgcctggca
     2641 gttcttctac tggaccttct gccgcatcga aatcgatccg gggatgtcgg gagttctcat
     2701 cgccaaaatg ggaacagatc tcccgccagg cgaaatcatc gccacgcaga cagggtacaa
     2761 gggcattcaa tttgacgtgc tgtctgaagg ccgccatttt tacaac
//
LOCUS       DAORHD010000605         2805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42161_length_2805_cov_3.015636, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000605 DAORHD010000000
VERSION     DAORHD010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42161_length_2805_cov_3.015636"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PKO06_12855"
     CDS             <1..462
                     /locus_tag="PKO06_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70583.1"
                     /translation="GAVAGDGLRVGLDLLVGEPDPVQPDSGRTVWFSPADGIVTGNGQ
                     FSADSVTSGLSEESWRANTLVRPGIYWVVALYRTLSPLRTATTVSLLARTSSVQKTTS
                     KSGLLPGQSYTAFRIQVNTSAVTIETVPEPELSESIRSSIRSWSRQNSSRK"
     gene            complement(557..>2805)
                     /locus_tag="PKO06_12860"
     CDS             complement(557..>2805)
                     /locus_tag="PKO06_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70584.1"
                     /translation="TASVSGADADAPTVTTQASAATESAKKSPWDWFKGAFEKAKQKF
                     LGTPGKEMPLWEKALWSIGKALIPSFGVMAITALLAPLSPVLMIVGGIVTGAVLGGAM
                     TYAFEKRMNAKYRETPKEDAKIWRDVAVGATVEAVMAPFQLATGGLFGMVGPTVGSAI
                     YRVAATQAALSFAGSALSASAGGAVKHLWAKHIFHYPEKIASNEARIDAILESRMQSG
                     SPLSEADVQELDRLQKEIDQMKAEDYGPDDFKKDMKRAALSSVISGFVGSVVADRAYT
                     YDSGRWADRLSVRVFGSAARGKEISSLLSTMPVNFASGMAGATLEKHFVSQDIDKLQK
                     QQAEYAPGTAGYQYYDGLIKQMQAKRQDVNVVGAGLDTMLNSTAVRAGQLTVQAIKYN
                     FIDAPMAKKRTLEQQYRENDPEWKKASTKYDEYQKIAEGGPDIRKYRNPVAYAKALKE
                     HNAKVEVARQKWLDQCLVAQNYEKTAAAQAVKTEMAASYDRNLKLNQLIELGRLTGGM
                     AHVRAVGELLKEQNPELAKLPENELQKLAIQSIRDSYTAKHVQCVEKVEKIESVRKQY
                     QEYKAGRLQLTEAEARVLEGRNALISPSQYKAALVEAKVYELKNQNVRWDDVKAQMPG
                     ILQSAENQMVNAYGGWGGVLGSEMYANGLAKYQYDPEGGKGLMAEAKKWFTKAPTTIA
                     KSTIDTYRSEVDSQLTSGLTPNSTDAEFEKLMQSFARGAITETTKQTSESIYKLSPQQ
                     IIKAFNRR"
BASE COUNT          535 a          861 c          736 g          673 t
ORIGIN      
        1 ggcgctgtcg caggcgatgg cttgcgcgta ggtctcgacc tgctggtcgg tgaacccgac
       61 ccggtgcagc ctgactcagg cagaaccgtg tggttttccc ccgcggacgg catcgtcacc
      121 ggaaacggac agttctcggc cgactccgtc acctccggac tcagtgaaga aagctggaga
      181 gccaatacgc tggtgcgacc tggaatctac tgggtggtcg ctctctatcg caccttgagt
      241 ccgttgcgca cggccacgac cgtgtcactt ttggcgcgaa cgtcgtccgt gcagaaaacg
      301 accagcaaat ccggtctgct gccagggcag tcctatacgg cattccgcat tcaggtcaac
      361 acgtcagccg tgacgatcga gaccgttccg gaacccgaat tgtccgagtc gattcgttcg
      421 tctatccgct catggagccg ccagaattca tctcgaaagt aggttgtttc ccgcgtcggc
      481 agccagaaac gtacaacgcc ttcggtggta agccgaaggc gttgtcgatt gtgcgtgagg
      541 cgtcgtcgtg aagaactcag cgccgattga acgctttgat gatctgctgc gggctgagct
      601 tatagatcga ttcgctggtc tgtttggtcg tttccgtgat ggcaccacga gcgaagctct
      661 gcatgagctt ttcgaactcg gcgtccgtgc tgttcggagt cagccctgaa gtcagctggc
      721 tgtccacctc tgagcggtag gtatcaatag tgcttttcgc gatggtcgtc ggggctttgg
      781 taaaccactt tttcgcttcg gccatcaggc ctttgccacc ttcaggatca tactgatatt
      841 tcgccaggcc gttcgcatac atttcggaac caagcacgcc accccagccg ccataggcat
      901 tcaccatctg attttcagca gattgcagaa ttccaggcat ctgcgccttc acatcatccc
      961 agcgcacatt ctggtttttt aactcgtaca ccttggcttc aaccagagcc gctttatact
     1021 gggaagggct gatcaacgca ttcctgccct cgagcaccct ggcttcggcc tcggttaact
     1081 gcaaccgccc agctttgtat tcctgatact gtttgcgtac tgattcaatt ttctcgactt
     1141 tttcgacaca ctgcacgtgt ttggccgtgt acgaatcgcg aatcgactgg atcgccagtt
     1201 tctgcagttc gttttcggga agcttcgcca gttcagggtt ctgttccttc agcagttcgc
     1261 ccacggctcg cacatgagcc ataccacccg tgaggcgacc aagctcgatc agttggttca
     1321 acttcagatt gcggtcatac gatgcggcca tctccgtttt cacggcctga gccgccgcgg
     1381 ttttctcgta gttctgcgcc accagacact gatcgagcca cttctggcga gccacttcga
     1441 ccttcgcgtt gtgttctttc agcgcttttg cataggcaac agggtttcga tacttgcgaa
     1501 tgtccgggcc gccttcagcg atcttctggt attcatcata ttttgtcgag gcttttttcc
     1561 attccgggtc gttctcacga tactgctgtt cgagcgtacg ctttttggcc atcggagcat
     1621 cgatgaagtt gtatttgatc gcctgcaccg taagctggcc ggctcgcacg gccgtcgagt
     1681 tcagcatcgt atccagaccc gccccgacca cattgacatc ctgacgtttg gcctgcatct
     1741 gcttgatcaa gccatcgtag tattgatacc cggcggttcc aggggcatac tcggcctgtt
     1801 gcttctgcaa tttgtcgatg tcctgactga caaagtgctt ttcgagcgtc gcaccggcca
     1861 tgccactggc aaagttcacc ggcatggtcg agagcagact cgagatctcc ttgcctcgcg
     1921 ccgccgagcc aaaaacccgc accgaaagac gatctgccca gcgcccggag tcatacgtgt
     1981 acgcgcggtc agccacgaca gagccgacaa aaccgctgat gacggacgag agcgccgccc
     2041 gcttcatgtc tttcttgaaa tcgtcgggac cgtagtcttc cgccttcatc tgatcgattt
     2101 ccttctgcag gcggtcgagt tcctggacat cggcttcgga cagcgggctg cccgactgca
     2161 tgcgcgattc cagaatggca tcaatgcgcg cctcattcga cgcgattttt tcggggtaat
     2221 gaaagatatg tttcgcccac aggtgcttca ccgctccacc agccgaagcg gatagtgccg
     2281 accccgcgaa actcagagcc gcctgggtgg cagccacgcg atagatcgcc gacccgacgg
     2341 ttggccccac catgccgaac aagccgcccg tagccaactg aaacggagcc atgactgctt
     2401 ctacggtcgc tccgaccgcc acatcacgcc agatcttggc atcttctttc ggcgtctcac
     2461 gatacttggc attcatacgt ttctcgaacg cataggtcat cgcgccgccg agcacagcgc
     2521 cggtcacaat gccgccgaca atcatgagca cgggggacag aggtgccagc aatgccgtaa
     2581 ttgccatgac cccaaacgac ggaataagtg ctttgccgat gctccagagt gccttctccc
     2641 aaagaggcat ctcttttccg ggggtgccga gaaacttctg tttcgctttc tcgaatgctc
     2701 ccttgaacca gtcccacgga gactttttcg ccgactctgt cgcggcagaa gcctgagtcg
     2761 tcaccgtcgg cgcatccgca tccgctcctg aaacgctcgc ggttt
//
LOCUS       DAORHD010000606         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42209_length_2803_cov_2.782751, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000606 DAORHD010000000
VERSION     DAORHD010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42209_length_2803_cov_2.782751"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1234
                     /locus_tag="PKO06_12865"
     CDS             35..1234
                     /locus_tag="PKO06_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019217204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70585.1"
                     /translation="MRECIAKADEQEYPRRVELLSGSSIKPENIRWLWRDWLAKGKLH
                     ILGGAPGCGKTTLAMVLAATVSSGGQWPDGSLCERGNVVIWTGEDDPADTLIPRLILS
                     GADLSRVHFINSVVDGEERRAFDPAHDVMLLAQTLRKAGNVRLLIVDPIVSAVAGDSH
                     KNAEVRRGLQGLVDLSREVGCALVGITHLSKGTQGREPLERITGSLAFGALTRIAWVA
                     VKPQPEEDGRMPSRLFLRVKSNMGPDGGGFEYELLQGETETGVRSSYALWGKAIDGDA
                     RTILSEAESVDDDGEGGALSHAKTFLRELLGNGPLVAKVIEKEAKEAGYSWSTIRRAQ
                     SALGIRPAKDAFNGPWKWSLPKLLKDTEDAHTKNVSIFDNFERLREKSAVGQPNPVAP
                     ERVEVEV"
     gene            1231..1617
                     /locus_tag="PKO06_12870"
     CDS             1231..1617
                     /locus_tag="PKO06_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70586.1"
                     /translation="MTPLEILRQSAADGVQIIVSHDGNLRTAGIRSSVEKWLSAIRDK
                     KADIVDYLTRVEGGGDLPRAVQTAALLVDLVPEADAKITAALDDLLRAELSRDPAAIR
                     AGVVNLELLVLRVWRYYQTKAEERAA"
     gene            1614..2081
                     /locus_tag="PKO06_12875"
     CDS             1614..2081
                     /locus_tag="PKO06_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70587.1"
                     /translation="MKKLPTIITTRNECALGVLAFYDLVRKLEDMGIVFRLSKTGFTV
                     TRDLGPQLDEVFAKNVTCVCSAVIMRTIERGLEYAFKREKQLAKLYPWLEMNFVDRRS
                     AIRETIDDDAFSLKEKCVAVKDYVMLVREAEKMHSKRTSTTVTTIEPGKPPGV"
     gene            complement(2137..2601)
                     /locus_tag="PKO06_12880"
     CDS             complement(2137..2601)
                     /locus_tag="PKO06_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70588.1"
                     /translation="MNQKFSDATRELSSILFHQTGLPSDEVARLLAVPVSTLKRWWQK
                     ETKPPHRGGSGTKPVSAIEALQTEIAALRIENAVLREKLTSKESNPIDNRVLIDELKN
                     RMRETIPTARLGEVATAIKTMIEVQKDDGASGDEARIIEYHIPAGEERPPIN"
BASE COUNT          637 a          667 c          848 g          651 t
ORIGIN      
        1 caacgacctt gcacaattcc ggtgcctcaa agcggtgagg gagtgcatcg cgaaggcgga
       61 tgaacaagaa tacccccggc gggttgagtt gctgagtggt tcgagcataa agcctgaaaa
      121 catccgttgg ttgtggcgcg actggttagc aaagggaaaa ctccatatcc ttggaggtgc
      181 tcccggttgc ggaaagacaa ccctggcgat ggtcctagcg gcaaccgtgt cgagcggagg
      241 tcagtggcct gatgggagtc tttgcgaacg tggaaacgtg gtgatctgga cgggagagga
      301 tgacccagcc gacaccctta taccccggct gatcctatct ggtgccgatc tgagccgagt
      361 gcacttcatc aactccgtgg ttgatggcga agaaaggcga gcctttgacc cagcccacga
      421 cgtcatgctt cttgctcaga cactgcgcaa agcggggaac gttcggctat tgatcgtcga
      481 tccgattgtc tctgctgttg caggcgactc tcacaaaaac gctgaggtga ggcgaggctt
      541 gcagggtctc gttgacctat ctcgtgaggt cgggtgtgcg ctggtgggaa tcactcatct
      601 gagcaaggga acccaagggc gtgagccatt ggaacgaatc accggaagcc ttgcctttgg
      661 agcattaacc cgaattgcgt gggtggctgt taaaccacag cctgaagaag atggcaggat
      721 gccaagccgt cttttcttgc gggtgaagtc gaacatgggc cctgatggtg gtgggtttga
      781 gtatgagttg cttcaaggtg agacggagac cggggttcgt tcgtcgtatg cgctatgggg
      841 caaggcgatt gacggagacg ccaggacgat tttatctgag gccgagtctg ttgatgatga
      901 cggagaaggt ggagcgctct cccatgcgaa gaccttcctc cgggaacttc tgggaaacgg
      961 ccctttggta gcgaaagtga tcgagaagga agccaaggaa gccgggtatt cttggtcgac
     1021 gattcggagg gcacaatcag ccctcggtat tcgaccggcg aaggatgctt tcaacggacc
     1081 gtggaagtgg tccttgccga agttgctcaa agataccgaa gatgctcaca caaaaaacgt
     1141 gagcatcttc gacaattttg agcgacttcg agagaagtcg gctgtcggac aaccgaaccc
     1201 cgttgccccc gaacgtgtgg aggttgaagt atgacacctc tcgaaatcct cagacaatct
     1261 gccgccgatg gcgttcaaat cattgtgtct catgacggga acttgagaac ggcgggaatc
     1321 cgatcttcgg tcgaaaaatg gttgtcggcg atccgtgaca aaaaggcgga tatcgtcgac
     1381 tacctgaccc gggtcgaggg cggaggcgat ctgccccgag cagttcagac ggcggctctg
     1441 ttggtcgatc tggtgcccga agctgacgcg aaaatcacgg cggctctcga tgatctgctt
     1501 cgggcggaac tctcgagaga cccggctgcg atccgcgcgg gggttgtcaa ccttgaactt
     1561 ctggttctcc gagtttggcg atactaccag acgaaggcgg aggaacgcgc ggcatgaaga
     1621 agctacccac aataatcacg actagaaatg agtgcgccct gggcgttctt gccttctacg
     1681 acctggtgag aaagctcgaa gacatgggga ttgtttttcg gctctcgaaa accgggttca
     1741 cggtgacgcg ggatctgggg ccgcaattgg acgaagtatt tgcaaaaaat gtcacgtgtg
     1801 tatgttctgc tgtcattatg cgaactattg aacggggctt ggagtatgca ttcaaacggg
     1861 aaaagcagct tgccaaactt tacccttggc ttgaaatgaa ctttgtcgac aggcgatcgg
     1921 cgatccgtga gaccattgat gatgatgcct tttcgctgaa agagaagtgc gttgcagtaa
     1981 aagactatgt gatgctggtc agagaggccg agaaaatgca ttcaaaacgg acaagcacca
     2041 cggtcaccac catcgaacca ggaaaaccac caggcgttta gcgactgttc gttccctgcg
     2101 gtgttgctct cagagctaaa agccagccct aaaagctcag ttgatgggcg ggcgttcttc
     2161 ccctgccggg atgtggtatt cgatgatgcg tgcttcgtcg ccacttgcgc catcatcttt
     2221 ctgaacctcg atcatggttt tgatggcggt ggctacttca cccaggcgtg cggttggaat
     2281 ggtctcacgc atccggtttt tcagttcatc gatcagaacc cggttatcga tcgggttgct
     2341 ttctttcgag gtcagttttt ctcggagaac ggcgttctcg atccgaagcg cggcgatctc
     2401 cgtttgaagg gcttcaatgg cgctcactgg tttggtgcct gacccgcctc gatgcggtgg
     2461 cttggtctct ttctgccacc atcgcttgag tgtggatact ggaacggcga gcaggcgggc
     2521 tacttcgtcg gagggtaggc cagtctggtg aaagaggatt gaagagagtt ctcgggtagc
     2581 gtcagaaaac ttctggttca ttgttgtttc cttgtgacag gcttcaatag atgattcttt
     2641 cattattgta gcctcaatcg cgcaactatg caaaaaatgc ggggtcgcga gtgtcaacag
     2701 gggaccggtt gttgacgaaa tacaccttga ctgtatttat gtaaatacat aacatatgta
     2761 ttatagacga gattcgcagg agggttcacc atgaaagctg tgg
//
LOCUS       DAORHD010000607         2800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42268_length_2800_cov_2.796721, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000607 DAORHD010000000
VERSION     DAORHD010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42268_length_2800_cov_2.796721"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="PKO06_12885"
     CDS             <1..1921
                     /locus_tag="PKO06_12885"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV70589.1"
                     /translation="WAVIVGDVKTDEALEIVKKTMGSAPKVAPPEDTIPAEPKQDGAR
                     EVVKYGDIQQAYVSIAWPAPSIESPDQYAMDLFSSIMGHGRSSRLHQALVEKERLATS
                     ISTSYFTAKDPHLLRIDVTCPQSNVRRLIDRVNKLLQEAAIAGVTQEELDKAKQMMIA
                     STIFSKETAEAQAGHYGYYATLGKLDEADRYIARLREATLDDVKRVATQYPIERTRNV
                     VRYEPQPASDSAKPQLFTLENGVRLILRENHSSPLVGVSVQIDAGGLREGKGQAGLAN
                     LTAKTLLKGTEKRSAEEIHQLLDRLGTRLNVLPGKSVVSLEMRTLTENFLPSVELLTE
                     ILTQPAFDESEIRIEQEKALENIKEEMDELFPRTLYLTLDKLFPDYPLAYSSLGLVDQ
                     LRKLKRSDVTGFHKKQYVGSGIVVAVVGDFFTAEVRDRLGGLLGTIPEGDLPELKEKD
                     FPAISKPVDLTDKLNREQAQVMVAFRTFPKKDSRGPAMDIIEAILSGSMSSRLFTQLR
                     DKESLAYSVSGNALGSRATGYFYATMSTKAAQADHARTRLIEELDTFGKKGFTEEEFA
                     DAKNYILGQHALSLVGNLEQASTFSYDEYMGLGFDYFEKYPALIRQTTKDEVLKLAGE
                     FFLGTHGYITGTTRP"
     gene            1949..2413
                     /locus_tag="PKO06_12890"
     CDS             1949..2413
                     /locus_tag="PKO06_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70590.1"
                     /translation="MNIELNEKELKTFVELLDIADTVLTSFEVDLNRKEKKPYIKLIQ
                     KLYRLAQQNGLEKWFEERKEVGELFLGEEYEKVAQAPDLIEEYEEAVFWEMLETELAH
                     RDLEEKHDKSEFEKDPYEALEYVLKYQEVYRHEFEKSGIQNMRLVKDSAKKK"
     gene            2585..2740
                     /locus_tag="PKO06_12895"
     CDS             2585..2740
                     /locus_tag="PKO06_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70591.1"
                     /translation="MQVLDEVDDAKAQLNREQPNKNRYDKKYTTNQGVHGRPRGKPDQ
                     LTFSALT"
BASE COUNT          677 a          818 c          778 g          527 t
ORIGIN      
        1 gtgggctgtc atcgtcggcg acgtgaagac cgatgaagca ctcgagattg tcaagaagac
       61 catgggctcc gcacccaagg tcgctccgcc cgaagacacg attccggcag aaccgaagca
      121 ggatggcgct cgcgaagtcg tgaagtacgg cgacattcag caggcctatg tttcgatcgc
      181 ctggccggct ccaagcatcg aaagcccgga ccaatatgcc atggatctgt tctcgagcat
      241 catgggtcat ggccgttcat cgcgtctgca tcaggctctc gttgagaaag agcggctcgc
      301 gacctcgatc tcgaccagct actttaccgc taaagacccg catctgctgc gcatcgacgt
      361 gacctgtccc caaagcaatg tgcgacgact catcgatcgg gtcaacaaac tgcttcagga
      421 agccgccatc gcgggagtca cccaggaaga gctcgataaa gccaagcaga tgatgatcgc
      481 ctcaaccatc ttcagcaaag agaccgccga ggcacaggcg gggcattatg ggtactacgc
      541 aacgctcggc aaactcgacg aagccgacag gtacatcgcc cgcctgcgcg aagcgacact
      601 tgacgacgtc aagcgtgtgg ccacgcagta tccgatcgaa cgtacccgca atgtcgtgcg
      661 ctatgagccg caacctgcct cggattcagc caagccgcag ttgttcacgc tcgaaaacgg
      721 cgtgcgcctg attctgcgcg aaaaccacag ctcacctctc gtcggcgttt cggttcagat
      781 cgatgccggt ggtctgcgcg aaggcaaagg tcaggccggg ctcgccaacc tgacggccaa
      841 gaccctgctc aaaggcaccg agaaacgctc ggccgaagag attcatcagc tgctcgatcg
      901 tctcggcacg cgattgaatg tcctccctgg caagagcgtt gtttcgctcg agatgcgcac
      961 tctcacggaa aactttctgc cgtcggtgga actgctcacg gaaatcctca cccagccggc
     1021 ctttgacgaa agcgagattc gcatcgagca ggaaaaagct ctcgaaaaca tcaaggaaga
     1081 gatggacgaa ctctttccgc gcaccctgta tctcacgctc gacaagcttt tcccagacta
     1141 tccgctggcc tactcgtcgc tggggcttgt cgatcagctg cgcaagctca aacgttccga
     1201 cgtcaccggg ttccataaga agcagtatgt cggctcgggc atcgtggtgg ccgtggtcgg
     1261 cgatttcttc accgccgaag tgcgcgaccg tctcggcggc ctgctgggca cgattcccga
     1321 aggagatctt ccggagctga aagagaaaga ctttccggcg atcagcaagc ctgttgatct
     1381 gaccgacaag ctgaatcgcg aacaggcaca ggtgatggtc gccttccgca ccttcccgaa
     1441 aaaagattct cgcggcccag ccatggatat catcgaagcc attctttccg gcagcatgtc
     1501 gtcgcgtctg ttcacgcagc tgcgtgacaa ggaatctctc gcctacagcg tcagcggcaa
     1561 tgcgctgggc tcacgcgcca ccgggtattt ttacgccacc atgagtacca aggcggctca
     1621 ggctgatcac gcgcgcacgc gactgattga agaactggac actttcggca aaaagggctt
     1681 caccgaggaa gagtttgccg atgccaaaaa ttacattctc ggacagcacg ctctcagtct
     1741 ggtcggcaat ctcgaacagg ccagcacctt cagttacgat gaatacatgg ggctgggctt
     1801 cgactatttc gagaagtatc cggcgctgat tcgtcagaca accaaggacg aagtcctgaa
     1861 actcgccggc gagttctttc tcggcactca cgggtacatc accggtacca cccgtccctg
     1921 atcgcgacca tcattgaaag gacctttcat gaacatcgaa ctgaacgaga aagaactgaa
     1981 aaccttcgtc gaactgctcg acatcgctga cacggtgttg accagcttcg aggtggatct
     2041 gaaccgcaag gaaaagaagc cgtacatcaa actgattcag aagctgtacc gtctcgccca
     2101 gcagaacggg ctcgagaagt ggttcgaaga gcgcaaggaa gtgggtgaac tcttcctcgg
     2161 cgaggagtat gagaaggtcg cccaggcgcc cgacctgatc gaagaatacg aagaggccgt
     2221 cttctgggaa atgctcgaaa ccgaactggc acatcgtgac ctcgaagaga agcatgacaa
     2281 gtccgagttc gagaaagacc cctacgaggc cctcgaatac gtgctgaagt atcaggaggt
     2341 ctaccgccac gagttcgaga agagcggcat tcagaacatg cgtctggtca aggattcagc
     2401 gaaaaagaag taggaccttc ggcacggcgc ggcaggcact gccgcgccga tgcttgcatg
     2461 ctcggtgtcg catatcgtat gtcgcgcacg gtgccgaaaa gtcagaggcg ggggttgccc
     2521 cccgcctctg tattctcaag acttgccgtc tcgtgcgcct ccagaaggag tcctctcacg
     2581 aggaatgcaa gtgcttgatg aagtcgatga tgcgaaagcc cagttgaacc gagaacagcc
     2641 caacaagaat cggtacgata aaaagtatac cactaatcag ggggttcatg gaagacctcg
     2701 cggaaaaccg gatcagctga ccttttcagc gctgacctga gcccgctttt tcatggcgtc
     2761 gatcagcacc gggccgagat cggtgacatt gcccgcgccg
//
LOCUS       DAORHD010000608         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42338_length_2797_cov_2.587527, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000608 DAORHD010000000
VERSION     DAORHD010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42338_length_2797_cov_2.587527"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKO06_12900"
     CDS             complement(<1..234)
                     /locus_tag="PKO06_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70592.1"
                     /translation="MRCTISPIVFLIVLLFGFLSIASTGQAALASGPVQPTSGPGGST
                     YPHASWRVVEIGEWETKCYIYEPQEPRPASAPVL"
     gene            343..1107
                     /locus_tag="PKO06_12905"
     CDS             343..1107
                     /locus_tag="PKO06_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNV70593.1"
                     /translation="MSTEKSVLMEHMNTVDLNHHLNAGGLVFLPVGTLEPHGRHLPVG
                     TDTICAREVAVELAKRLSGIVAPPLGYGLTNLLAQTPPASFFPEPLFQAFVDAILEGL
                     AQHGFSPLVIVNGHGGNRDALKAVARAFIKRRAVALSVIHWWHFAEPPAKKIYGATGG
                     HAANEETAAMLFFDSQLVRRDQYSPDEDDYTPSDAMWCYPPPGEVLIYHDDPRGRPDF
                     DRAKARSVMEQTIDAIEARLKSWMSRIRRLGGGLRP"
     gene            1271..1855
                     /locus_tag="PKO06_12910"
     CDS             1271..1855
                     /locus_tag="PKO06_12910"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV70594.1"
                     /translation="MNFWKVVLIVALLLSPVSSLVYAGCGTCPGDEAKSDCSTCESKS
                     DCGTACGAEPAAADPAKVTPTEPAKTEPAKVEEKGHEHTAFPTVTGEELSKLIADKVP
                     VVILDARSGKWDDGKRIPGAKQLTDTATPEEVAKLVPAKDAKVVTYCSNLKCPASLHL
                     AEHLKKLGYTNVVKYPGGLEDWLKSGKEVVSEKK"
     gene            1985..>2797
                     /locus_tag="PKO06_12915"
     CDS             1985..>2797
                     /locus_tag="PKO06_12915"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNV70595.1"
                     /translation="MAHLTTRLSFLFLLLTALVTLPASAEIVTLVNGDLLDVDRIAYQ
                     GDMLNVTLGTETRQLPRSQVRDIAFSGKKRERDVLATDTQDLQLMLKQGQELLTQFPD
                     SLAILVRDENDFFHRVDGTNVYRIRAAYLLAKEEALGVAQVGLGFDPVRQRIKVLYAR
                     SYSPDGEVSALSADQIKISKGSSSGEYFNQNQMVGFTIPGAKVGCVIDYAYEIEEFNP
                     FDKNLFQGSNYFQGQQPVVLSVMRVFFPKDTPLNYKAYRLDDAAAKPVVTEEG"
BASE COUNT          602 a          756 c          821 g          618 t
ORIGIN      
        1 cagcacggga gcgctcgcgg ggcggggctc ctggggctca tagatgtaac acttggtttc
       61 ccattcgccg atttcgacga cgcgccagga agcgtgcgga taggtactgc cgccgggccc
      121 cgaagtcggt tgaaccggcc ctgaagccag cgccgcctgt cccgtcgatg cgatgctgag
      181 aaagccgaag agcagaacga tcaggaatac gatgggcgaa attgtgcatc tcatgcgcgc
      241 atcttacctg aactgcgacg gcttcgccaa gtcaccggct ggaccttaac cgggaattct
      301 gttacatcac tctcaagcag gacatgcatg gaggtttgcc acgtgagtac ggaaaagtcg
      361 gttctgatgg aacatatgaa cacggtggat ctgaatcacc atctcaatgc aggcgggctg
      421 gtgtttctgc cggtcggaac actcgaacct catggtcgac atcttcccgt gggcacggat
      481 acgatttgtg cacgagaggt ggccgttgag ctggccaagc gcttgtcggg tatcgttgcc
      541 ccgccgctcg ggtatggact cacaaatctg ctggcgcaga caccgccggc aagctttttc
      601 cccgagccgc tgtttcaggc gtttgtcgat gccattctgg aaggactggc tcaacacggg
      661 ttctcgccac tcgtgatcgt gaacggtcat ggcggcaatc gcgatgcgct caaagcggtc
      721 gctcgggcct tcatcaaacg aagggcggtc gccttgagcg tgattcactg gtggcatttt
      781 gccgagccgc cggcaaagaa gatctacggc gcgacgggag gccacgcggc gaacgaagag
      841 acggccgcca tgctattctt cgactcgcaa ctggtgcggc gagatcagta ttcccctgat
      901 gaagatgact atacgccgag tgatgccatg tggtgctacc cgccgcccgg cgaagtgctt
      961 atctaccatg atgatccacg cggtcgcccc gacttcgacc gtgcaaaagc ccgttcggtc
     1021 atggaacaaa cgatcgatgc cattgaggcg cggctgaaat cctggatgtc tcgaattcgt
     1081 cgactgggtg gggggttgcg accgtagaaa gagaaatttc actccggcac ttgatttttc
     1141 acgtcgctca agtatattat tagcaaagac taattaagtt agatttaact aataattaaa
     1201 gaagcggttg atacgctgaa aacggtaagt ccgtgctgaa caacatcaaa tctgaaatcg
     1261 gaggcccgat atgaatttct ggaaagtcgt tctgattgtc gccctgttgc tgtcccctgt
     1321 gtcgtccctg gtgtatgcag gctgtggcac gtgtcccggc gatgaggcga agtctgattg
     1381 ctcgacctgc gaaagcaagt ctgactgtgg cactgcctgt ggtgccgagc ctgctgctgc
     1441 cgacccggcg aaggtaaccc cgaccgagcc cgccaagacc gagcccgcga aagtcgaaga
     1501 gaagggccat gagcacaccg catttccgac ggtgactggc gaagagctgt caaagctgat
     1561 cgctgacaag gttccggtcg tgattctcga cgcacgctca ggcaagtggg atgacggcaa
     1621 gcgcattccg ggcgccaagc agttgaccga tacggcaacg cccgaggaag tcgccaagct
     1681 ggttccagcg aaagatgcga aagtggtgac ctattgttcc aacctgaaat gtcctgcctc
     1741 tctgcacctg gccgagcatc tgaagaagct gggctacacg aacgtggtga agtatcccgg
     1801 cggactggaa gattggttga agtccggcaa ggaagttgtg tccgagaaga agtagcgttc
     1861 ctcctccaac tcctcctccc tacctcctgt cctcgagccc cggaatgttc cggggctttt
     1921 ttgttttgtc ttgatacctg cttccccgta taatgcggga gaacttcatg tcccggagga
     1981 aaccgtggcc catctgacga cgcgtttgtc tttcctgttc ctgcttttga ctgccttggt
     2041 caccctgcca gcgagtgctg aaatcgtgac tctggtgaat ggcgacctgc tggatgtcga
     2101 tcgtatcgcg tatcagggcg atatgctcaa tgtcacgctg gggacggaga cgcgccagct
     2161 tccccgctct caggtgcgcg acatcgcttt ttcgggaaag aagcgtgaac gtgatgtcct
     2221 ggccaccgac actcaagatc ttcagttgat gctgaaacag ggacaggaac tcctgaccca
     2281 gtttcccgac agtctcgcga tcctggttcg ggatgagaac gattttttcc atcgtgtcga
     2341 cggaacgaac gtctatcgca ttcgcgcggc ctatctactg gccaaggaag aggctttggg
     2401 cgttgcccag gtaggacttg gcttcgatcc ggtgcgccaa cgcatcaagg ttctgtatgc
     2461 gcgcagttac tcgccggacg gagaggtgtc agcgctgagt gccgatcaga tcaaaatttc
     2521 caaaggcagt tccagcggcg agtatttcaa tcagaaccag atggtcgggt tcacgattcc
     2581 cggtgccaag gttggctgtg tcatcgatta tgcgtatgaa atcgaagaat tcaacccctt
     2641 tgacaagaat ctgtttcaag ggtcgaacta ctttcagggg cagcaaccgg tggtgctctc
     2701 ggtcatgcgc gtgtttttcc cgaaagatac tccgttgaat tacaaggcct accgtctgga
     2761 tgacgccgcg gcgaagccgg tggtaaccga ggagggc
//
LOCUS       DAORHD010000609         2794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42396_length_2794_cov_3.698795, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000609 DAORHD010000000
VERSION     DAORHD010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42396_length_2794_cov_3.698795"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..700
                     /locus_tag="PKO06_12920"
     CDS             200..700
                     /locus_tag="PKO06_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70596.1"
                     /translation="MAISVASLVMLPIILLFGVTEKVTYKSINEVVASNLALQKIEEL
                     KSRPFEKLREIIETQSPDPIDGPFAVVKLPREFNDQWNTPGVEYSREAALSFYPYPNP
                     DATAVDYELQKRRIRIRIAVDFTELVLDKKRVKTFEMSTIVTDETLGSGLNASFTNPV
                     PNSGGN"
     gene            700..1419
                     /locus_tag="PKO06_12925"
     CDS             700..1419
                     /locus_tag="PKO06_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70597.1"
                     /translation="MILPTRSVLRRTRGVTFVELLISLLLLGIVFTIGYTLLQRSLQS
                     IDRQKQSLDTLHEARTFLMVVERDLREMTRLIELDTVFKDDLFNPENAFFYSMTLEIP
                     DRRAATGTTTVTYTYQGPKNYSSEGNLPKFIYRQEKIGERKALITKQLAYLKVWGTDG
                     TIFRNRLAEESNAAYASYLRSHFYHPQNNAANGLRSLDKVRGIEVELSMNELFDVQGK
                     PVKTRNFRTRIYSRVLNSKFD"
     gene            1448..>2794
                     /gene="alaS"
                     /locus_tag="PKO06_12930"
     CDS             1448..>2794
                     /gene="alaS"
                     /locus_tag="PKO06_12930"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNV70598.1"
                     /translation="MNTATIRSKFLDFFGRQHHVVKNSFSILPQDDPTLLFISAGMAP
                     FKPYFLGLKTDIKRAATCQKCFRTTDIDNVGYTFRHHTFFEMLGNFSFGDYFKKEAIA
                     WAWEFITKDLGLPKDRLHVSVHHSDDEAYQIWNRDMGVPADHLVKLGDKDNFWTIGVG
                     PSGPCSEIYIDQGPALGCGDPKCAPGCDCARYLEFWNLVFTQYNRAEDGTLTPLEKKN
                     IDTGMGLERLAAIMQGKTDNYDNDVFQGIVRHVEEITHRRYNDSTQIRTAMRVVGDHV
                     RALSVALADGAMPGNEGRGYVLRKILRRAARFGYRYLGQEKPFLYQIVPTISRILDVY
                     PEIQQNLDHVSRVIHGEEERFLVTLKTGSEILTDFIGRLRKDGKAELDGGRAFLLHDT
                     YGFPLDLTREICREEKMTVDEKGFATELEKQRERGRANAVSAFVNFSDINPSQYPAT"
BASE COUNT          652 a          747 c          760 g          635 t
ORIGIN      
        1 ggatagggcg atgaaggaaa ccagaaccag tcccatgttt ttgcgatcga ttttcattgg
       61 ccttacctcg ttattagcat gtcctgtctt tcagaaaacc tatcgacgtt tcgtgggcaa
      121 acctttatgc cctcgtgccg aaaaaaatca aaaatcgtgc caacatgagc gcctcggcat
      181 caccctggtt gaaatactgg tggcgatctc ggttgcctca ctggtcatgc tgccgatcat
      241 tctgctgttc ggagtgacgg agaaagtcac ctacaagagc atcaatgagg ttgtcgccag
      301 taatcttgcc ctgcagaaaa tcgaagaatt gaaaagccga ccatttgaaa agctgcgaga
      361 aatcatcgaa actcagtcgc cggacccgat tgacggaccg ttcgcggtcg tcaagctgcc
      421 gcgcgaattc aacgatcaat ggaacacgcc gggagtcgag tactcacgcg aagcggcgct
      481 gtcgttctac ccgtacccga atccggatgc gaccgctgtg gattacgagc tgcagaagcg
      541 ccgtatccgc attcggatcg cggttgattt tacagagctg gttctggaca agaagcgcgt
      601 gaaaacgttt gaaatgtcca cgattgtcac tgatgaaacg ttgggttcgg gcctgaatgc
      661 gagctttacc aaccccgtcc ccaattctgg aggcaactga tgatcctccc cacacggtct
      721 gttttacgcc ggactcgcgg tgtcacgttc gtcgaactgc tcatttcact gttgctgctg
      781 gggatcgtct tcaccatcgg ctacacgctg ctgcagagat cgctccagag tatcgatcgg
      841 cagaaacagt cgctcgatac gttgcacgag gcgcgtactt ttctgatggt cgtggagcga
      901 gatctgcgcg aaatgacgcg tctcatcgaa ttggacacgg tcttcaagga cgacctgttc
      961 aatcccgaga acgccttttt ctacagtatg accctcgaga tccctgaccg gcgtgcggcg
     1021 acgggcacga ccactgttac ctacacctac caggggccca agaattattc ctcggaagga
     1081 aatcttccga aattcatcta tcgtcaggaa aagatcggtg agcggaaagc cctgattacc
     1141 aagcagctgg cttacctcaa agtctggggt acggatggga cgattttccg caaccgtctc
     1201 gcagaagaaa gcaatgctgc ctatgcgtcg tatctgcgtt cgcatttcta tcatccgcag
     1261 aacaatgctg ccaacggcct gcgcagtctc gacaaggtgc gtgggatcga agttgaactc
     1321 tcgatgaatg agctgttcga tgtgcaggga aaaccggtca agacccgcaa tttccgcact
     1381 cgcatctatt cacgggtttt gaactcgaaa tttgattgac cccggataca ctccaaggag
     1441 ccctgtcatg aacactgcca ccattcgctc gaagtttctc gacttctttg gccgccaaca
     1501 ccatgtggtc aagaattcct tttcaatctt gccgcaggac gacccgacgc ttctattcat
     1561 cagtgccggc atggctccct tcaaaccgta ttttcttggc ctcaagacgg atatcaagcg
     1621 tgcggcaacc tgtcagaaat gttttcgcac gacggatatc gataacgtcg gctacacctt
     1681 ccgtcaccac accttctttg agatgctggg aaacttcagt tttggagact atttcaagaa
     1741 ggaagcgatc gcctgggcct gggagttcat cacgaaagac ctggggttgc ccaaagatcg
     1801 cctgcatgtt tcggtgcatc acagcgatga cgaagcctat cagatctgga accgcgacat
     1861 gggggtgcct gcagatcatc tggtgaagct gggtgacaag gataacttct ggacgattgg
     1921 ggtcggccca tcgggaccgt gttctgagat ctatattgat cagggaccgg ctctcgggtg
     1981 tggagatcca aagtgtgcgc ccggctgtga ttgtgcgcgc tatctcgagt tctggaacct
     2041 ggtgttcacg cagtacaacc gggctgaaga cggaaccctg accccgcttg aaaagaagaa
     2101 catcgatacg ggcatgggtc tcgaacgcct cgcggccatt atgcagggca agacagacaa
     2161 ctacgacaac gatgtctttc agggcattgt tcgccatgtt gaagagatca cccaccgcag
     2221 atacaacgac tcgacgcaga ttcgcacggc gatgcgcgtt gtcggcgatc acgtgcgggc
     2281 actgtcggtc gcgctggcag atggagcaat gccgggcaac gaaggccgcg gttacgtgtt
     2341 gcgcaagatt ctgcgccgtg ccgcgcggtt cggctatcgc tatctcggtc aggaaaagcc
     2401 tttcctgtat cagatcgtgc cgacgatttc acggattctc gatgtgtatc ccgagataca
     2461 gcagaatctc gaccacgtga gtcgcgtgat tcatggcgaa gaagaacgtt tcctggtgac
     2521 gttaaagacg ggctcggaga ttctgacgga cttcatcggt cgcctgcgga aggatggcaa
     2581 agcggaattg gacggtggtc gagcttttct gttgcatgac acgtatggct ttccgctcga
     2641 tctgacccgt gagatctgcc gtgaggaaaa gatgaccgtt gacgagaagg gcttcgcaac
     2701 cgagctggaa aaacagcgcg agcgcgggcg agccaacgcg gtatcagcat tcgtgaactt
     2761 ctctgacatc aatccatcgc agtacccggc cacg
//
LOCUS       DAORHD010000610         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42438_length_2793_cov_2.683346, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000610 DAORHD010000000
VERSION     DAORHD010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42438_length_2793_cov_2.683346"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKO06_12935"
     CDS             <1..969
                     /locus_tag="PKO06_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70599.1"
                     /translation="ALPLIDIRLAQRDPAEIGGIYVPDRDHGVLSLLPPEWVKRACAE
                     PCFVFLDELNAAVSKLHQAVAYQIVLEHRIGDFAFHPQTVVMAAGNLEEDRAIVSTLS
                     SALNNRFAHFVLRVDAKTWTEWARQHQIDAMLISFIERNGEEALYENTGESAFASPRS
                     WEMVSRVLKTVELNHRKRAVAACVGASMAELLFQQVKLFETFDAKAILVEGRPVNFRT
                     KPFAEPSIAQAAVFAVADLACGKDGNAEPDHLPNVVKFLRSPGLDPEFIMLFLRRVKE
                     KSNLYELLKTVPEFKVVAGDLVDIHGGYLKSMGAPLAAPFEAKCPF"
     gene            1079..2398
                     /locus_tag="PKO06_12940"
     CDS             1079..2398
                     /locus_tag="PKO06_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNV70600.1"
                     /translation="MNHIKEEQRDILAATELRRLAGVRLRMLDKQPFWGHLLMNLQFR
                     STFELPAPAATDCLRVIWFNPQFTAQLSFAQLGFLLAHEVGHIALLTQHRQNGRDSVV
                     WNHATDYAINRLVASTPDEPDVKRPLYEVPVFRRENGELMNLLLNPDFDDLPAEAIYQ
                     RLLLETPTRNVVIRLRLPGSHAGRQNDDQPQEQTPGNLDDGDLVIDGIVDHGGGLDVH
                     LPDWVTQRDLDEWQRMIREALGHWNRSGRRGTVPSDRLRQLANGGKLAARDWRALLRQ
                     HVERSLEQRDEYSRARPHRRYLLQDLIVPSVEGETVAELVIALDTSGSMNDQLLSSCL
                     QAVSQLSRVADQCLLLTADARVHDVIRTADIEAFVNKRNVTGGGGTDHRPVFTWLAER
                     QIVPALFIGITDLQSAFPKTKPEFPVLWLTPKWHWRAPWGTVIEVDL"
     gene            2481..>2793
                     /locus_tag="PKO06_12945"
     CDS             2481..>2793
                     /locus_tag="PKO06_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70601.1"
                     /translation="MNRNRVPVIVMLLLTVAMGCEASALLVTCEVGGATLERYNDTTR
                     SWQRIGGMPYLIAVRRYAYVMIRVTAPGYQEFEQGYEVRTDGTARAHVVLEPENDNPT
                     ST"
BASE COUNT          564 a          835 c          856 g          538 t
ORIGIN      
        1 gctctgccgc tcatcgacat tcgactcgcc cagcgcgacc cggccgaaat cggcggcatc
       61 tacgtgcccg accgcgacca cggcgtgctg tcgctgttgc cgcccgagtg ggtgaaacgc
      121 gcttgcgccg agccctgctt cgtttttctc gatgagctca acgcggccgt ctcgaaactg
      181 catcaagccg tcgcctacca gatcgtgctc gaacaccgca tcggtgactt cgcctttcac
      241 ccccagacgg tcgtcatggc cgccggcaac ctcgaagaag accgggcgat cgtctcgacc
      301 ctctcgtcag cgctcaacaa tcgttttgcc cactttgttc tgcgggtcga cgccaagacc
      361 tggacggaat gggcgagaca gcaccagatc gacgcgatgc tgatctcttt catcgagcgt
      421 aacggcgaag aggcgttgta tgaaaacacc ggtgaatcgg cgttcgcctc gccccgcagt
      481 tgggaaatgg tcagccgggt gctgaagacc gtcgaactga accaccgcaa acgcgccgtg
      541 gcggcgtgcg ttggcgcatc gatggccgaa ctgctgtttc agcaggtgaa gctgttcgag
      601 accttcgacg ccaaggcgat tctcgtcgag ggccgacccg tgaactttcg caccaagccg
      661 ttcgccgagc cttcgatcgc gcaggccgcc gtattcgccg tggccgatct cgcgtgcgga
      721 aaggacggca acgccgaacc ggaccatctg ccgaacgtcg tgaagtttct acggtcaccg
      781 gggctcgacc cggagttcat catgctcttt ctgaggcggg taaaggagaa aagcaacttg
      841 tacgaattgc tgaagacggt gccggaattc aaagtcgtgg caggcgatct ggtcgatatc
      901 cacggcggat acctgaagag catgggcgcg ccactcgccg cgccgttcga ggccaaatgc
      961 ccgttctgac cgctccatgc tctctgagtg ccgggcagtc tgcttcacgc tttttgactg
     1021 gtgaggcggt cgcaccgcac tggaaaacgc cgccttcaag accgaaggga gaagtcgcgt
     1081 gaatcacatc aaagaggaac aacgagatat actcgccgcg acagaactga gacggctggc
     1141 gggcgtgcgg ctgcgtatgc tcgacaaaca gcctttctgg gggcatctgc tgatgaacct
     1201 gcagtttcga tcgacgttcg agctgccggc gcccgccgcg accgactgcc tgcgcgtgat
     1261 ctggttcaat ccgcagttta ctgcccagct ctcattcgct cagctcggct ttctgctggc
     1321 gcacgaggtc ggacacattg ccctgctcac tcagcatcgt cagaacggcc gcgacagcgt
     1381 ggtctggaac catgccaccg actacgccat caatcgcttg gtcgcgagta ctccggacga
     1441 acccgacgta aaacgcccgc tctatgaagt accggtcttt cggcgtgaga acggtgaatt
     1501 gatgaacctg ttgctgaacc cggactttga cgacttgccc gctgaggcca tctatcagcg
     1561 ccttctactc gagacgccga cgagaaacgt cgtgattcgt ctgcgccttc ccggttcaca
     1621 tgcaggccgg cagaacgacg atcaaccgca ggaacaaacg cccggtaacc tggacgatgg
     1681 cgatctggtg atcgacggca tcgtcgacca cgggggcggt ctcgatgtgc atctgccaga
     1741 ctgggtgact cagcgcgacc tcgatgagtg gcagagaatg atacgggaag cgctcggaca
     1801 ctggaaccgg tcggggcgac gcggtacggt gccgagcgat cgtctgcgtc agctcgcgaa
     1861 cggtggcaag ctcgccgcgc gtgactggcg ggctctcttg cgacagcatg tggaacgttc
     1921 gctcgaacag cgcgatgaat actcgcgtgc ccggccgcat cgccggtact tgctccagga
     1981 tctgatcgtg ccgagcgttg aaggcgagac cgtcgccgaa ctggtgatcg cgctcgacac
     2041 gtcgggcagt atgaacgacc agttgctaag ctcttgtctg caggctgttt cacaactgtc
     2101 tcgcgttgcc gaccagtgcc ttctgctgac cgccgatgca cgcgtgcatg acgtgattcg
     2161 caccgctgac atcgaggcct tcgtgaacaa gcgaaacgtc acaggcgggg gaggcacgga
     2221 tcatcgaccg gtgttcacgt ggctggccga gcgacagatt gtaccggctc tgttcatcgg
     2281 catcactgat ctgcaaagcg catttccgaa aacgaaaccg gagtttccgg tgctctggct
     2341 gacccccaaa tggcactggc gagccccctg gggcaccgtc atcgaagtcg atctgtgagt
     2401 cattttcgga tgacttgtga aggaatactt tctcggcagg ttcagtgaag gtgaccacga
     2461 aaccaacaca ggaggggcct atgaaccgga acagagttcc cgtgatcgtg atgcttctgc
     2521 tgacggtggc gatgggatgt gaggcgagcg cgttgctggt gacctgcgag gtcggtgggg
     2581 caacgctcga acgatacaac gatacgacgc ggagttggca gcgcatcggc ggaatgccgt
     2641 atctgatcgc ggtgcggaga tatgcctacg tgatgatccg cgtgacggcg cctggatacc
     2701 aggaattcga gcaggggtat gaagtacgaa ccgatggaac ggcgcgtgct catgtggtgc
     2761 tcgaacccga gaatgacaac ccaacctcaa cgt
//
LOCUS       DAORHD010000611         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42518_length_2790_cov_3.525046, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000611 DAORHD010000000
VERSION     DAORHD010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42518_length_2790_cov_3.525046"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PKO06_12950"
     CDS             <1..386
                     /locus_tag="PKO06_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HNV70602.1"
                     /translation="PVSEGVFMEGLVNFFEQAEFQDPAKIKRMMGLLDRKDYLLNVLG
                     QSLENGDDIMVNIGSSSGLGEEGLSVVTARYQGPNQSFGRIGIIGPTRMDYGRVVATL
                     VNISKALSELFLGKETFGAGNRKRF"
     gene            413..1066
                     /gene="grpE"
                     /locus_tag="PKO06_12955"
     CDS             413..1066
                     /gene="grpE"
                     /locus_tag="PKO06_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV70603.1"
                     /translation="MEPTNNTQTGAGADASTKAGEAFEGKQTPQQPGSSDAPPTAAGS
                     AGDVPPAPEKDSPGPASAPASELEKELSRVKKELDEAQKSYLRARADYDNLKRRSAND
                     FLNASSEGAIGIVKKLLPVLDSFESALKQLQNANVDKKFRDGVEMLYMQFSDTLEKEG
                     LKPIAAKGKKFDPNEHEAMTRGQTDEYEDEVVMQEFVKGYKFRERVIRLASVQVNSK"
     gene            1095..>2790
                     /gene="dnaK"
                     /locus_tag="PKO06_12960"
     CDS             1095..>2790
                     /gene="dnaK"
                     /locus_tag="PKO06_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNV70604.1"
                     /translation="MGRVIGIDLGTSNSAAAVIEGGKPVIIPSAEGISIGGKAFPSIV
                     AFAKDGQMIVGEPARRQAAMNPDRTILKIKRKMGTNAKTKVGDKEFTPQQISAFILQK
                     IKRDAEAFLNDKVDGAVITVPAYFDDAQRQATKDAGAIAGIEVKRIINEPTAAALAYG
                     LDKKGDHKILVFDLGGGTLDVTILEMGNGVFEVLSTNGDTQLGGTDMDESILKYITAE
                     FKANEGIDLTKDSMAMMRLREAAEKAKIELSNTTTTDINLPFITATADGPKHLVMTIT
                     RSKLEELIDPIVSRCRAPIDNALRDAKMTRDQVEHIIMVGGPTRMPIIQKLLKDHFGK
                     EPERGIDPMECVAMGAAIQAGVLQGEVKDVLLLDVTPLSLGIETLGGVMTKLIDRNTT
                     IPTKKSQIFSTASDNQPAVSIHVLQGERPMAADNKTLGRFDLVGIPPAPRGVPQIEVT
                     FDIDANGITHVSAKDMATGKEQKITITSSHALNKDEIDKMVKEAEKYAQEDAKVKEKI
                     DAKNQAESLIFNAEKSLKELGDKVPADLKVTIEGKISELKKALEGNDVEDIKKKTEE"
BASE COUNT          725 a          723 c          814 g          528 t
ORIGIN      
        1 gtccggtctc cgaaggtgtt ttcatggaag gtctggtgaa ctttttcgaa caggctgagt
       61 tccaggaccc ggcaaagatc aagcgcatga tgggattgct cgaccgcaag gactatctgc
      121 ttaatgtttt gggccagagc cttgaaaacg gtgatgacat catggtaaac attggctctt
      181 cctctggttt gggggaagag ggactttcgg tcgtgacggc tcgctatcag gggccgaatc
      241 aatcattcgg tcgtatcggc atcattgggc ctacccgcat ggattacggt cgtgtcgtgg
      301 cgacgctggt caacatctcg aaagctctca gtgaactgtt tctcggcaaa gagacattcg
      361 gtgccgggaa ccgaaagcgt ttttgaacgg tcaaactcaa cgaggaacga ttatggaacc
      421 gacgaataat acccagacgg gtgcgggtgc tgatgcttcg accaaggccg gagaggcttt
      481 cgaaggaaag cagacgccgc agcaaccagg ctcttctgac gctccgccca ctgcggccgg
      541 aagcgcaggt gatgtgccgc cggctcctga aaaagactct cccggccccg cgtctgcgcc
      601 tgcgtccgag ttggagaaag agcttagtcg cgtcaaaaaa gaacttgacg aggctcagaa
      661 gtcctacctt cgggctcgcg ccgactacga taatttgaaa agacgctcgg ccaatgattt
      721 tctgaatgcc tcgagcgaag gggcgattgg catcgtcaag aaactgctgc cggtactcga
      781 cagttttgag tcggctctga agcagctgca gaacgccaat gtcgacaaga agtttcgcga
      841 cggggtcgaa atgctgtaca tgcagttttc cgataccctc gaaaaagaag gattgaagcc
      901 gattgccgcg aagggcaaga agttcgatcc gaacgaacat gaagcgatga cccgcggtca
      961 aacggatgaa tacgaagacg aagtggtcat gcaggaattc gtgaaagggt acaagttccg
     1021 tgaacgtgtg atccgcctgg caagcgtgca ggtgaattcg aaataatacc tcgatgaagg
     1081 aggaataaga agatatgggt cgtgtcatcg gaatcgattt agggacgtcc aactcagcgg
     1141 cagccgtgat cgaaggcggc aagccggtca tcatccccag cgcggaagga atttccatcg
     1201 gtggaaaggc ttttccgagc attgtggctt ttgccaaaga cggtcagatg atcgtgggcg
     1261 agcccgcccg tcgtcaggcc gccatgaacc ctgaccgcac gattctcaag atcaagcgga
     1321 agatgggaac caacgccaag accaaggtgg gcgacaagga attcacgccg cagcagatct
     1381 cggcattcat tctgcagaag atcaagcgcg acgccgaagc ctttctgaat gacaaggtcg
     1441 acggcgctgt cattacggtt ccggcctact ttgacgacgc gcagcgtcag gccacgaaag
     1501 atgcgggcgc gatcgccggt atcgaagtca agcgcatcat caacgaaccg acggccgcgg
     1561 ctctcgccta tggtctcgac aagaagggcg accacaagat tcttgtattc gacctgggcg
     1621 gtggaacgct cgatgtcacc attctcgaaa tgggcaacgg cgtgtttgag gtgctctcaa
     1681 ccaatggcga cacgcagctt ggcggcactg acatggatga atccatcctg aaatacatca
     1741 cggccgagtt caaggcaaac gaaggcatcg atctgaccaa ggattcgatg gcgatgatgc
     1801 gtctgcgtga agcggctgag aaagccaaga tcgagctgtc gaacaccacg acgaccgaca
     1861 ttaacctgcc gttcattacg gctacggcgg acggtccgaa acacctggtc atgacaatca
     1921 cccgctcgaa acttgaagaa ctgatcgatc cgatcgtttc ccgctgccgc gctccgatcg
     1981 acaatgcgct gcgcgatgcg aagatgacgc gcgatcaggt cgagcacatc atcatggtcg
     2041 gtggtccgac tcgcatgccg atcatccaga aactgctcaa agaccatttc ggcaaagaac
     2101 cggaacgcgg aatcgatccg atggaatgtg tggcaatggg tgccgcgatt caggccggcg
     2161 tgctgcaggg cgaggtgaaa gacgttctgc tgctcgacgt aacgcctctc tctctcggca
     2221 tcgagacgct cggcggagtt atgaccaagc tcatcgaccg caatacgacc attccgacaa
     2281 aaaagagcca gatcttttcg acagccagcg ataatcaacc tgcagtgtcg attcatgtgc
     2341 tgcagggtga acgcccgatg gccgctgata acaagaccct gggtcgcttt gatctggtcg
     2401 gcattccgcc ggcaccgcgt ggtgtgccgc agatcgaagt cacgtttgac attgatgcca
     2461 acggcattac gcatgtctcg gccaaggaca tggccaccgg aaaagagcag aagatcacga
     2521 tcacgtcgag tcacgccctg aacaaggatg agatcgacaa gatggtgaag gaagccgaaa
     2581 aatacgctca ggaagacgcg aaggtcaaag agaagatcga tgccaagaac caggccgaaa
     2641 gcctgatctt taacgccgag aagtcgttga aggaactggg cgacaaggtt cctgccgatc
     2701 tgaaggtgac gatcgaaggc aagatcagcg aattgaagaa agccctcgaa ggcaacgacg
     2761 tcgaggacat caagaagaag accgaagaac
//
LOCUS       DAORHD010000612         2790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42523_length_2790_cov_2.888848, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000612 DAORHD010000000
VERSION     DAORHD010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42523_length_2790_cov_2.888848"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..1529
                     /locus_tag="PKO06_12965"
     CDS             117..1529
                     /locus_tag="PKO06_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70605.1"
                     /translation="MNFKLTMVDTHPYGKNDFGPKTVDGVTIEQKLAKINPEFYYAYP
                     VYNYSTKDGIKVTDFSSANNDIMKVGMLDFHNSPSGHGTPIEFKGTKYDVQWVWKKAT
                     TVNATTKNQVTEQKNGLDVGGTWTVEGTVNLGVPTPWHVVSDSTEYTAHSKTYPKPVF
                     KVFCLCEDSAGHKWPVFGNFIDDTSKTTAAEEWTEMKNPETGGDYKQSILAADKQLAG
                     SPDIADIVQAKTGNAGTDFSWQQVASYTAEDKSKPEIQVIVFDTRNNSYHIFGSRNGG
                     DALAATKPNAPVNYTGMTPAPFSTTDLDTLTGEYKFETMDVGLFETFFKTTSQKGVNL
                     LDDHKGHGIVCQKNTRLIFYIRAWDNVNTFNDAEKFGINTLEFKVVDATWNGGADEQP
                     PASDRTPYSTSYDLANLTTNPPMWQFRADNVDSGADCHLEVKATDKAGNATDFMLKVF
                     VTGKEIEIRSLEEKRNRLSN"
     gene            complement(1770..>2790)
                     /locus_tag="PKO06_12970"
     CDS             complement(1770..>2790)
                     /locus_tag="PKO06_12970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70606.1"
                     /translation="FAKVIKDKLYGRFDEAFSAFPECVDLNPDYLNGNDEGVIIAGLN
                     FYQDKLERGDQSALFMHSVYLYFKGDFFGSEQGLYKFIETKPSKEELERAQKYLKLIA
                     DRREAQAKALVHEPPKKTPNKTPIKTIDKAPKKDPATSTAAVTTGVSGETGSTALAPS
                     DTSNLPPLDDGNVVVHAPHLEHATTKMLKDFDGDQASVVEALSLAANYRIPDPEIVSK
                     IADIVETTDNDMIRAHALDALSRIGAPAQSSLPQITRHLDSDNFFVRSLALNAAANSG
                     GKPEELIPLFVPSFNSKSPAVRYTAIQATVKMGSRAVPALEEALKDATGQAEKSIKQA
                     LKLMK"
BASE COUNT          671 a          680 c          774 g          665 t
ORIGIN      
        1 aacccagtgc atccagtggt ttcacggctt tcaaggtcta cgacacacga cctccggttt
       61 cgtcgaaact cgaactgaca cctgtggcgc agtcatcggg tgaggcgatg acgaccatga
      121 atttcaaact gacaatggtc gacacgcatc cgtacgggaa aaacgatttt ggcccgaaga
      181 ctgttgacgg agtcacaatt gaacagaagt tagcgaagat taaccctgaa ttctattatg
      241 cttaccctgt ctataattac agtacgaaag atggaataaa agttaccgat ttctcaagtg
      301 cgaacaatga cattatgaaa gtgggaatgt tggacttcca caatagtcct tccggtcacg
      361 gcacacccat tgaattcaag gggacaaaat acgatgtcca gtgggtctgg aagaaagcta
      421 ccacggtgaa tgccaccacg aaaaaccagg tgactgagca gaagaatggc ctggacgttg
      481 ggggaacatg gaccgtggaa gggacagtga atcttggagt cccgactccg tggcacgtgg
      541 tgtcagattc tacagagtat accgctcaca gcaagacgta tcctaagccg gtcttcaaag
      601 ttttttgtct gtgtgaagat tctgcgggtc acaaatggcc agtcttcggg aacttcattg
      661 acgatacctc gaagaccact gccgccgagg aatggaccga aatgaagaac ccggaaacgg
      721 gcggtgatta caagcaatcg attcttgccg cagacaagca gcttgctgga agccctgata
      781 ttgctgacat cgttcaagcc aagacgggaa atgccggcac ggacttttcc tggcagcagg
      841 ttgcgagtta cacggctgaa gacaagagca agcctgagat tcaagtcatt gtcttcgata
      901 cgcgcaacaa cagctaccac attttcggtt cgcgcaacgg tggtgatgcc ctggctgcta
      961 ccaaacccaa cgctccggtg aactacacgg gtatgactcc cgccccattc agcaccacag
     1021 acttggatac cctgacaggc gagtacaagt ttgagacgat ggatgtcgga ttgtttgaga
     1081 cgttcttcaa gacgacgagc cagaaggggg ttaacctgct ggatgatcat aaaggccatg
     1141 gcatcgtctg ccagaagaac acgcgcttga tcttctacat tcgggcttgg gacaacgtga
     1201 acaccttcaa cgatgccgaa aagttcggga tcaacaccct ggaattcaaa gtcgttgacg
     1261 caacctggaa tgggggagcc gacgaacaac ccccggccag cgatcgtact ccctactcaa
     1321 cctcgtatga tctggccaat ctcacgacca acccgccgat gtggcagttc cgtgctgata
     1381 acgtcgattc tggcgctgac tgccatctcg aagtgaaagc cactgataaa gcggggaacg
     1441 ctacggactt catgttgaag gtgtttgtga ccggtaaaga gatcgagatt cgctcgcttg
     1501 aagagaagcg taatcggtta agtaattgat cgtcgatcgt ttgatcaggg gtgaaaacgc
     1561 tctcgttttc acccctgatt tttgtacgcc aatgagaatg gttccggtgt gtgggctgct
     1621 gccgcgttgg cacagagatg agaaatcctc tgggtgagaa agtacgggtt cggaacagca
     1681 agagtatgca atggaacatc atcaggggga gtgctaaaga aaaaccgcga cggtaggttg
     1741 ttttgtaccg tcgcggtttc gttccgcagc tacttcatca gtttcaaagc ctgcttgata
     1801 cttttctccg cctgacccgt ggcgtccttc agtgcctctt cgagtgctgg tactgcacgc
     1861 gaacccatct tcactgttgc ttgaattgcc gtatagcgta cggctggact tttactattg
     1921 aagctgggga cgaacaaagg aatcaattcc tccggtttgc cgcctgaatt ggctgcggca
     1981 ttgagagcca ggctgcggac gaagaaattg tccgagtcga gatggcgtgt gatctgcggc
     2041 agggaactct gtgctggagc accaattctg gacaaggcat caagcgcatg agccctgatc
     2101 atgtcattat cagtggtttc gacgatgtcg gcaattttac tgacaatctc ggggtctgga
     2161 atacgatagt ttgccgcaag gctgagagct tcgacaacgg aagcctggtc tccgtcaaag
     2221 tctttcagca tcttcgtcgt ggcgtgttca aggtgagggg cgtgaaccac gacgtttccg
     2281 tcatccaggg gcggcaggtt cgaggtgtcg cttggcgcca gtgccgttga gcctgtctca
     2341 ccgctcactc ccgtggtgac ggcggccgtg gaggtggccg ggtctttttt cggggctttg
     2401 tcgatggttt tgatcggtgt tttgttcggg gttttcttgg gtggttcgtg taccagagct
     2461 ttggcttgag cctcgcggcg gtcggcaatg agtttgagat acttctgtgc ccgctcaagt
     2521 tcttccttgg agggtttggt ctcgatgaac ttgtacaatc cctgctctga tccgaaaaag
     2581 tcgcccttga aataaagata gaccgagtgc atgaacaaag cgctctggtc accgcgttcc
     2641 agtttgtcct ggtagaagtt gaggccggcg atgatgacgc cctcatcgtt cccattcaaa
     2701 tagtcagggt tgaggtcgac gcactcggga aaggcactga atgcctcatc gaatcgccca
     2761 tacagtttgt ccttgatgac cttggcgaag
//
LOCUS       DAORHD010000613         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42545_length_2789_cov_3.216167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000613 DAORHD010000000
VERSION     DAORHD010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42545_length_2789_cov_3.216167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..1212
                     /locus_tag="PKO06_12975"
     CDS             154..1212
                     /locus_tag="PKO06_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNV70607.1"
                     /translation="MFDTLTLKHLLQHCLQRGASDLHLSVGIPPAARIHGEIVMLPFD
                     NLDPQTCQLLIFSTLNDEQIARFDQDWELDFSLTIPELGRFRVNVFRERGYVEASFRI
                     VTPEIKSLAELGLSEVATDLARRPNGLVLVTGPTGVGKTTTFNSLIDQINRERRCRII
                     TIEDPIEYMHKHKKSVVLQREVHSDTRSFPVALRHILRQDPDIIGIGEMRDLETIATA
                     ITAAETGHLVIATLHTCDAQQTIDRIVDVFPPHQQEQVRVQLANSLQGIISQQLLPNV
                     EKNGRVLAYELMIVTPAIRRLIRENKIPQIDTFIQTGSEFGMISMDKSLKTLYQQGRI
                     AYDIAMSKVKNPAAFKEL"
     gene            1212..2183
                     /locus_tag="PKO06_12980"
     CDS             1212..2183
                     /locus_tag="PKO06_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV70608.1"
                     /translation="MQRNLFPSQEHLRGFSRALFSTWLYHRRFNILFDAGEGVATMLL
                     NRVFGIRRVFLSHGHADHIAGLINLINLRNLGAGDQTATLEIYYPRGNRLIELMKQYL
                     AQTQNELSFSLEWRPIEAGQVIDLDPRRGKIFLRSFATQHSSKQLSLGFNIVEKRRRL
                     RPEFMGKTQLEINELIRHQGKEQIAENFEQIIFSYGGDSRPINPEQVAGSLLLCHECT
                     YLKLEDDERNFQQHSVLEEVLEVARRAQVKGLLLFHLSLRYSFDEIRQMVNQTVERVH
                     PDCRVLILYGDRFFDPTDTTSRRRFREEVVDEEEESEVIVMREEIEE"
     gene            2180..>2789
                     /locus_tag="PKO06_12985"
     CDS             2180..>2789
                     /locus_tag="PKO06_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70609.1"
                     /translation="MNKPKILVIDDEHSVRWAFEKALQKAGYAVHLAETGARGLAIWE
                     TVHPDLTLVDIRMPEMDGLQVLERIRTKDPKARVIVMTAYSDMDTTVTAMKHGAYDFL
                     SKPFNIDQCLELIRKGLEARTAEKAAQPTEQPVKSGGIIGNSPAMREVYKLIGKVSAE
                     DITVLITGESGTGKELVARAIHENSRRADKPFWAINCTAVAES"
BASE COUNT          637 a          766 c          812 g          574 t
ORIGIN      
        1 gtcgaagcca tcacttatcc tcccgaccga gcgcgtcggc tgcgcgattg aggggcacga
       61 aaagagcccg gtcgtttgcg cgaacgcgct atcgacaaac cgcgaaaaaa ccgttaccgt
      121 cattctcaga atcgatgcga tggagagttc tcgatgtttg atacgttgac cctgaaacat
      181 ctgctgcagc actgcctcca gagaggtgca tcagacctgc atctctccgt gggcattcct
      241 ccggccgcgc gcattcacgg cgagattgtc atgttaccct ttgacaacct cgacccgcag
      301 acctgtcagt tgctgatctt ttcgacgctg aacgacgagc agatcgcccg tttcgatcag
      361 gactgggaac tcgatttctc tctgaccatc cccgaactcg gccgcttccg ggtgaatgtg
      421 tttcgtgaac gcgggtatgt ggaagcctct ttccgcatcg tgactcccga aatcaagtcg
      481 cttgcggaat tgggactttc tgaagtcgct accgatctgg ctcgaagacc aaatggactg
      541 gtactcgtga ccggaccgac cggcgtgggc aaaacgacca cgttcaactc gctcatcgat
      601 cagatcaacc gtgagcggcg ctgtcgcatc atcaccatcg aagatcccat cgagtatatg
      661 cacaaacaca agaagtccgt ggtgctgcag cgtgaagtgc attccgatac tcggtcgttc
      721 ccggtggcgt tgcggcacat tctgcgccag gacccggata tcatcggcat cggggagatg
      781 cgcgatctgg agacgattgc caccgcgatc acggcggccg aaaccggaca tctggtcatc
      841 gcgacgctgc acacctgcga cgcgcagcag acgatcgatc gcattgtcga cgtcttcccg
      901 ccgcatcagc aggagcaggt gcgcgtccag ttggcgaact ccctgcaggg catcatttca
      961 cagcagctct tgccgaacgt ggaaaaaaat ggcagagtgt tggcctacga actgatgatt
     1021 gtgactcccg cgattcggcg tctgatcagg gagaacaaga tcccgcagat cgatacgttc
     1081 attcagaccg gaagtgagtt cggcatgatt tcgatggata agtcactgaa gacgctgtat
     1141 cagcaggggc gcatcgccta tgatatcgcc atgtcgaaag tgaaaaaccc ggcagccttc
     1201 aaggaactgt gatgcagcgg aacctctttc cttcacagga gcatctgcgc ggcttttccc
     1261 gggcgttgtt ctcgacctgg ttgtaccacc gacgcttcaa catcctgttc gatgcgggcg
     1321 aaggggtcgc caccatgttg ctgaaccggg tcttcgggat tcgccgcgtg tttctgtcac
     1381 acgggcatgc cgaccacatt gccggtctca tcaatctgat caatctgcga aatctcggcg
     1441 cgggtgatca gaccgcaacc ctcgagattt actacccgcg tggaaatcgc ctgattgaac
     1501 tgatgaaaca gtatctggcg caaacccaga acgaattgtc gttctctctg gaatggcgcc
     1561 cgatcgaagc cggacaagtc atcgacctgg acccaagacg ggggaagatc tttctgcgca
     1621 gctttgccac gcagcactcg tcgaaacagt tgagtctggg gttcaacatc gtggagaagc
     1681 ggcgtcgact gcgtccagag ttcatgggaa aaacgcagct cgaaatcaat gagctgattc
     1741 gccaccaggg caaggaacag attgccgaga acttcgaaca gatcatcttt tcctatggtg
     1801 gtgattctcg accgatcaac cccgagcaag tcgccggcag tctcctgctg tgtcatgaat
     1861 gtacctacct gaaactggaa gatgatgaac gcaatttcca gcagcactcg gtgctcgaag
     1921 aggtgctcga ggttgcccgg cgcgcgcagg tcaaggggtt gctgctgttc cacctgtcgc
     1981 ttcgctactc gttcgatgag atccggcaga tggtgaatca gacggtcgag cgtgttcatc
     2041 cggactgccg ggtgctgatt ctgtatggag atcgtttttt tgaccccacc gacactacga
     2101 gtcgccgccg gttcagggaa gaggtggtcg acgaagagga agagtcggaa gtgatcgtca
     2161 tgcgtgagga gatcgaagag tgaataagcc gaagattctg gtcatcgacg acgagcacag
     2221 tgtgcgatgg gcctttgaaa aagccctgca gaaggcgggt tacgcggtgc atctggccga
     2281 gaccggcgcg cgtgggttgg cgatctggga aacggttcat ccggatctca cgctggtgga
     2341 tatccgcatg ccggagatgg atggcctgca ggtgctcgaa cgcatcagaa ccaaagatcc
     2401 gaaggcgcgt gtcatcgtca tgaccgccta cagcgacatg gacacaacgg taaccgcgat
     2461 gaagcatggc gcgtatgact ttctgagcaa gccgttcaat atcgaccagt gcctcgaact
     2521 gattcgaaaa ggcctcgagg cgcgtacggc cgaaaaggcg gcgcaaccaa ctgaacagcc
     2581 ggtgaagagc gggggcatta tcggaaacag cccggctatg cgcgaagtgt acaagttgat
     2641 cgggaaggtg agtgctgaag atatcacggt tctcattacc ggggaatcgg gaacgggcaa
     2701 agagctcgtg gcgcgcgcca ttcacgagaa ctcacgccgt gccgacaagc cattctgggc
     2761 gatcaactgc acggcggtag ccgagagtt
//
LOCUS       DAORHD010000614         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42648_length_2784_cov_2.727006, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000614 DAORHD010000000
VERSION     DAORHD010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42648_length_2784_cov_2.727006"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKO06_12990"
     CDS             <1..411
                     /locus_tag="PKO06_12990"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV70610.1"
                     /translation="VRDIHTLVAGVLHDTIEDTGTSRDELTAQFGADIAAIVQELSDD
                     KSLPKEERKRLQIEHAKHISLAAKQIKIADKITNVHDVAYSPGVGWSLKRREEYISWT
                     EKVVANARGANAKLEAAYDRTVQDARRILQETKE"
     gene            421..891
                     /locus_tag="PKO06_12995"
     CDS             421..891
                     /locus_tag="PKO06_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70611.1"
                     /translation="MSITEKEVVTIKELLTVIPEIRQQFGVSRNPDVKEALLYCGVAE
                     SNIIPALTPLIEKYFGKAYKPAGSWAFFKNWFDPFVKAIGGIRTDQTLFKKDIRENLW
                     VYCAFWPWGSNPVKTSIRIGIICETDEEEDYLDKQLKSSFSGAAPAQATTEAKS"
     gene            1042..1878
                     /locus_tag="PKO06_13000"
     CDS             1042..1878
                     /locus_tag="PKO06_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HNV70612.1"
                     /translation="MNLSTVTAAEIAQLDREQTIVFLPLGPAEQHGPHLPIGTKVFIA
                     EALAFEASNFLKPAGLTCLTAPSLPFAPCQVSFGIPGTFSLNARAFSDFLFEIGQSFQ
                     REGFRWFFLVNHSVSLECLKACETAISDLNRLRDFHAFDPLPALLQARTPQLEQELKN
                     LGCAAGRELHADARETSAMMYLDADLVDGKVLPQLPPHLPNLSFESLKGNFSYVDMGA
                     KNGYVGTPSAGTPEFGKLYIETAAAGLAESVRKALAGQPLPQLPIPIRMLLKLVDLDE
                     TL"
     gene            1893..2240
                     /locus_tag="PKO06_13005"
     CDS             1893..2240
                     /locus_tag="PKO06_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70613.1"
                     /translation="MVKLRDLNRECSARPVSRAVPAQVACRLLGNNTKPCPLDALCKP
                     AGKHGVWFFYSVLASVACTALLISFWALHITRLGWVLSLIGRVLLPPLRWAVSAVIAV
                     YNLCVSVAEQFED"
     gene            2260..>2784
                     /locus_tag="PKO06_13010"
     CDS             2260..>2784
                     /locus_tag="PKO06_13010"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV70614.1"
                     /translation="MTVFYNCVGHDLPDYAKKSFDSFVVHDGRIDELGWELHTFSRFS
                     HATKVDLGGRDVFPAFADAHTHFLQTGLMLSGCDLSSARSISDVLAQLDEFARSCSDS
                     WLLAWNLDETLLKENRLPTVNELDRVIPRHKAWFSRIDLHSAVPNSATMRWAAEKHGS
                     APLENGRYVKRTYEI"
BASE COUNT          595 a          813 c          746 g          630 t
ORIGIN      
        1 gttcgcgata ttcatacgct ggtcgccgga gttctgcacg ataccatcga ggacaccggt
       61 acgagtcgtg atgagttgac agcccaattt ggcgcggata tcgccgcaat tgttcaagaa
      121 ctttcagatg acaagagcct gcccaaggaa gaacgaaagc gcctgcagat cgaacacgcc
      181 aagcatattt ctctggcggc gaagcagatc aagatcgctg ataagataac gaatgttcat
      241 gacgtcgctt actctccggg tgtgggctgg tctctcaagc gacgagaaga atacatatcc
      301 tggactgaga aagtcgtggc gaatgcccgt ggagcgaacg ccaaactcga agcagcctat
      361 gatcgcaccg ttcaggacgc acgtcgcatc cttcaggaaa ccaaagagta ggggaacacc
      421 gtgagtatca ccgaaaaaga agtcgtcacc atcaaggaac tcctgaccgt gattcccgag
      481 attcgccagc agttcggcgt ctcgcgtaac ccggatgtca aagaagctct tctgtactgc
      541 ggtgttgccg agtcgaatat tattccggcc ctgacgccgt tgatcgaaaa atacttcggc
      601 aaggcttaca aacctgcggg ttcctgggca ttcttcaaga actggttcga cccctttgtg
      661 aaagccatcg gcggcattcg caccgatcag acgctgttca agaaagacat tcgtgagaac
      721 ctctgggtct actgcgcctt ttggccgtgg ggttcaaatc cggtcaagac ctcgatccgc
      781 atcggtatca tctgcgaaac cgatgaagaa gaggactatc tcgacaagca gctcaaatca
      841 agcttctcgg gcgcagcacc ggctcaggca accaccgaag ccaagagctg atcgtcgcgc
      901 tcgttcggta agcgatctgc ttccgcaaga aagagtgttt acataatata tattatcgga
      961 cgttttgcgc cgaaaacggg ttttcggact cgacatctgc tgcattgact cgcgcgccgg
     1021 accacgtgta ttatcggcgc aatgaatctt tccaccgtca cggccgctga aatcgcccaa
     1081 ctcgatcgcg aacagacgat cgtgtttctg ccgctgggcc ccgccgaaca gcatggtccg
     1141 catctgccga tcggcaccaa ggtgttcatt gccgaagccc tggcctttga ggcgtcgaat
     1201 tttctcaagc cggcgggtct gacctgcctg accgcgccat cgcttccctt cgctccctgc
     1261 caggtcagct tcggaattcc cggtaccttc tccctgaatg cccgcgcttt ttccgatttt
     1321 ttgtttgaaa tcggccagtc gtttcagcgt gaagggtttc gctggttctt tctcgtgaac
     1381 cacagcgtgt cgctcgagtg cctcaaagcc tgtgagacgg cgatcagcga cctcaaccgc
     1441 ctccgcgact ttcatgcctt cgacccgctg ccggcactgc tgcaggcccg aactccacaa
     1501 ctcgagcagg aactgaagaa ccttggctgt gccgcaggtc gtgaactgca cgccgacgct
     1561 cgggaaacct cggccatgat gtatctcgat gccgatctgg tcgacgggaa ggtgcttccg
     1621 cagttgccgc ctcatctgcc gaatctgtcg tttgagagct tgaaaggcaa cttcagctac
     1681 gtcgacatgg gagccaaaaa cgggtacgtc ggaacgccat cggcgggtac cccggagttc
     1741 gggaaactgt atatcgaaac cgcggcggcc ggattggcag agtcggtacg caaagcactg
     1801 gccgggcaac cattgcccca gctccccatt cccattcgca tgttgctcaa gcttgtcgac
     1861 ctcgacgaga ccctctagga accgaggcga acatggtcaa actgcgtgat ctgaatcgcg
     1921 aatgctctgc cagacccgta tcgcgggctg tcccggcaca ggtggcctgc cgcctgctcg
     1981 gcaataacac gaaaccctgc cctctcgatg cgttatgtaa accagcaggg aagcatgggg
     2041 tctggttttt ttactccgtc ctggccagcg ttgcctgtac ggcgcttctg atctcgttct
     2101 gggcacttca tatcacacgt cttggatggg tgctgtctct tatcgggcgt gtgctgcttc
     2161 cccccctgcg ctgggcggtg tcggcggtga tagccgttta taacctctgc gtttcggtcg
     2221 ctgagcagtt cgaagactga ccatcgctca ccggcatcca tgaccgtttt ctacaactgc
     2281 gtcggccacg accttcccga ttacgccaaa aaaagtttcg attcgtttgt cgtacatgac
     2341 gggcgcatcg atgaacttgg ctgggagtta cataccttct cccgcttttc ccatgcgacc
     2401 aaggtcgacc tcggcggcag agatgtcttt ccggcatttg ctgatgccca tacgcatttt
     2461 ctacagactg gtttgatgct gagcgggtgt gatctgtcga gtgcccgttc gatcagtgat
     2521 gtgcttgccc agcttgatga gtttgcgcgc tcctgttccg attcgtggct tctggcttgg
     2581 aatctggatg aaacactgct gaaggaaaac cgcctgccga ccgtcaacga actcgatcgc
     2641 gtcattcctc gacacaaggc ctggttctcg cgcatcgacc tgcattccgc cgttcccaac
     2701 agcgcaacca tgagatgggc cgctgaaaag cacggaagtg ccccgctcga aaacggacga
     2761 tacgtgaagc gcacgtatga gatt
//
LOCUS       DAORHD010000615         2783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42668_length_2783_cov_3.092742, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000615 DAORHD010000000
VERSION     DAORHD010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42668_length_2783_cov_3.092742"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="PKO06_13015"
     CDS             complement(<1..152)
                     /locus_tag="PKO06_13015"
                     /inference="COORDINATES: protein motif:HMM:NF037013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70615.1"
                     /translation="MIPTARLTLHFSPQPTPGLKTKALRGALATAFPEHDLLHQHNTS
                     GCLYRYP"
     gene            complement(149..2461)
                     /gene="cas3"
                     /locus_tag="PKO06_13020"
     CDS             complement(149..2461)
                     /gene="cas3"
                     /locus_tag="PKO06_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNV70616.1"
                     /translation="MTELRSHPHLHLSEHIEQVSQAISGILQWHTNSDEVRRLSQLAY
                     VAARAHDLGKGTSAFQDYIRNPTGFRGNHVLKEHTALSQLLVHLGEAFSEYSPLDHLI
                     LAMTAKAHHSGFPTVEELRDTFGGETAKCLRDQIPLIDFPGLQKETALTLSSTRFAGK
                     PWSSAKKQMDDRLIPALKALSLDDAWHYRLAAQFIYSLLLEADKVFLAVPDAQKHLTR
                     TPVDFDPGWVSWRTRDAPDSPINEIRTSARGKVIETWQNRTDVSMWALTAPTGIGKTL
                     LGATWAFHERRRRLEQTGIRPKIIIVLPFLTIIDQTVAEYQRLLGYGSTELDGATLLT
                     SHSLSDRQYGSRYDSATEAFFIDTWRSELIITTYDQFLYALFHPKSKHQMRFHHLIDA
                     LIIIDELQTLPCSLWDPFRHAAEILSERFNTRFLFMSATMPAFLPKAVSLYPDYQNLF
                     SQFNRYILRMSITTPRTLEQFTNDLSPRLKAWQEQGHKVMLTFNTRASARFVRDWAER
                     QIAHNNVPFSLYFVTADVAPIDRRRAVEAIRADARCLVISTQCVEAGVDLDLDLIIRD
                     FAPLDSLVQIAGRCNRNGRRDRGTVEIVDLVTTQGRRFSEYIYHPIHLQETRRLLAAR
                     NEIQESDILPLADDYFRALSTSRNLGQEWSKKHAYWESPPTTVKQLLRPEQGEQIEFI
                     VSKLDPGLREATSAIQDIEDRWERREAWRRIAGRIALVSVNVYLRKGFEPTDIAEEFC
                     GQWFLRDNHYDSEKGLVMANDTPDEGVLIL"
     gene            complement(2458..>2783)
                     /locus_tag="PKO06_13025"
     CDS             complement(2458..>2783)
                     /locus_tag="PKO06_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HNV70617.1"
                     /translation="ERRWHFTPCLGLSEMLADLVFEDTVTAQKLPMGIHEVGTVFPRE
                     LGELNTDALYERELALLALRMPHAVTADRVFSHRAYYMERMARPVPVQTDHAYQAGGT
                     IIAFL"
BASE COUNT          635 a          688 c          822 g          638 t
ORIGIN      
        1 ggataccgat acagacaacc tgaggtgttg tgctggtgaa gcaggtcgtg ttcgggaaaa
       61 gctgtcgcca gagcgccgcg cagagctttg gttttcaacc ccggggtcgg ctgtggactg
      121 aaatgcagtg tgaggcgagc tgtcggaatc acaggatgag cactccttcg tcaggtgtgt
      181 cgtttgccat gaccagtccc ttctcggaat cgtagtgatt gtctctcaga aaccattggc
      241 cgcagaattc ctcggcgatg tccgttggtt cgaacccttt tcgaagatag acattcacgg
      301 aaaccaaggc gattctgccg gcgattcttc gccaggcttc acgccgttcc cagcgatctt
      361 cgatatcttg aatggccgat gttgcttcac gcaaaccggg gtcgagtttg gaaacaatga
      421 attcgatctg ctcaccttgc tcggggcgca acagttgttt gacggtggtc ggcgggctct
      481 cccaatacgc atgcttctta ctccactcct ggcccagatt tctcgatgtc gagagagctc
      541 gaaaatagtc gtcggccaag gggagaatat ctgactcctg aatctcgttt cgagcagcga
      601 gaagtcgccg agtctcttgg agatggatcg gatggtagat gtactctgaa aaacgacgcc
      661 cttgagtcgt gacgagatcg acaatttcaa ccgtccctcg gtcgcgtcgt ccgtttcgat
      721 tacaccggcc ggcaatctgc accaggctgt cgagtggcgc gaagtctctg ataataaggt
      781 cgagatcgag atcgacacca gcctcgacgc attgggtcga aatgaccaga cagcgagcgt
      841 ccgcgcgaat ggcttcgact gccctgcggc gatcgatggg tgcaacgtca gccgtcacga
      901 agtacaggga aaacggaaca ttgttgtgtg ctatctgcct ttcggcccag tcgcgcacaa
      961 atctggccga agcgcgggtg ttgaacgtca gcatcacctt gtgtccctgt tcctgccacg
     1021 ctttgaggcg aggtgagaga tcattggtga actgctcgag tgtccgtggg gttgtgatac
     1081 tcattcgcag aatgtaccgg ttgaactgtg aaaaaagatt ttggtaatca ggataaagcg
     1141 agacggcttt aggcaaaaaa gcgggcattg tcgccgacat gaacagaaaa cgtgtgttga
     1201 aacgttcgct cagaatttcg gccgcatggc ggaaagggtc ccagagggaa cacggcagag
     1261 tctgcagttc atcaatgatg atcagagcat caatcagatg gtgaaaacgc atctggtgtt
     1321 tcgatttcgg atgaaacagg gcatacagaa actggtcgta ggttgtaatg atcagctcgg
     1381 accgccaggt atcgatgaaa aacgcttcgg tggcggaatc gtatcgactt ccatattgcc
     1441 ggtctgacag agaatgcgag gttaagagag tcgcgccgtc gagctcggtt gagccatacc
     1501 cgagaagacg ttgatactcc gccaccgtct gatcgataat cgtcagaaac ggaaggacaa
     1561 tgatgatctt ggggcgtatg ccggtttgct cgagtcgccg acgtcgctca tgaaacgccc
     1621 aggttgcccc aagcagggtt ttcccaatgc ctgtcggggc agtcagggcc cacatgctta
     1681 cgtcagtccg attttgccat gtctctatga ctttgcctcg agcactggtt cgtatctcgt
     1741 tgatcggact gtcgggtgca tctctggttc gccatgaaac ccatcctgga tcgaaatcaa
     1801 caggtgttcg cgtcagatgt ttctgtgcgt caggaacggc aagaaagact ttgtcagcct
     1861 cgagaagcag ggagtatata aattgcgccg ctagacgata gtgccaggcg tcgtcaagcg
     1921 aaagagcttt taatgccgga atcaggcgat catccatctg tttcttggca gaagaccacg
     1981 gtttgccggc gaaacgtgtg ctggaaagag tgagtgcggt ttctttctgc aggccgggaa
     2041 aatcgatcaa cggaatctgg tcgcgcagac atttcgcggt ttcgcccccg aacgtgtcgc
     2101 gcagttcctc aaccgttgga aagccactgt gatgagcctt ggcagtcatg gcaagaatca
     2161 gatgatcgag cggtgagtac tcggaaaatg cctcacccaa atgcacaaga agctgagaaa
     2221 gcgctgtgtg ttctttgaga acgtgattgc cacgaaatcc ggtcgggttg cgaatgtagt
     2281 cttgaaatgc cgaggtgcct ttgccgagat cgtgtgcgcg tgccgcgacg taggctaact
     2341 gggacaaacg acgtacttca tcagagttgg tgtgccattg cagaatgccc gaaatggctt
     2401 gcgacacctg ctcgatatgc tcagagaggt gcagatgcgg gtgtgaacgt aattcggtca
     2461 caggaaggcg atgatcgttc cacccgcctg ataggcatga tcagtctgca cgggcactgg
     2521 tcgcgccatt cgctccatgt agtaggcgcg atggctgaag acgcggtcgg cagtgaccgc
     2581 gtgaggcatg cgaagtgcca gtagagcgag ttcgcgctcg tataaggcgt ctgtgttcaa
     2641 ttcgccgagt tcgcgcggaa acacggtgcc tacttcgtga atgcccatcg ggagtttctg
     2701 tgccgtcacg gtgtcttcga agacgagatc agcgagcatc tctgaaagac ccaggcaggg
     2761 ggtgaaatgc cagcgtctct cac
//
LOCUS       DAORHD010000616         2780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42725_length_2780_cov_3.823853, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000616 DAORHD010000000
VERSION     DAORHD010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42725_length_2780_cov_3.823853"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKO06_13030"
     CDS             complement(<1..330)
                     /locus_tag="PKO06_13030"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNV70618.1"
                     /translation="MDLSALELQLADVIASSTWLAPAAAFGGGVLTALNPCVLATVPL
                     IIGVVGGYAQGDGAWKRALGFSFFFVFGFALELAVLFSVSAAFAPYLTGPSWKYVIAA
                     ICFFLGLH"
     gene            complement(334..906)
                     /locus_tag="PKO06_13035"
     CDS             complement(334..906)
                     /locus_tag="PKO06_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV70619.1"
                     /translation="MIQLPRLIVVAVVTLLMALHLSQGSSSCLCGSPAFAQASESAPV
                     VEVPLTASATAPQPSAVVPTTQPASDPGKVAGVASESAAGSATATPAVVRGPLPKMVD
                     LGAGKCNACKKMEPVLEEAKRLYEGKADVIFIDVWKDRDAGSTYGIRMIPTQIFFDAN
                     GKEVYRHEGFLPLEDIRKQFESLGVVLLKE"
     gene            complement(928..1158)
                     /locus_tag="PKO06_13040"
     CDS             complement(928..1158)
                     /locus_tag="PKO06_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV70620.1"
                     /translation="MKIQILGTGCAKCTKLAELAKQAADELKLDYQLEKVTSLNDIMK
                     FGVMVTPALAVDGKVKVSGKLPSLDELKGFLK"
     gene            complement(1173..1481)
                     /locus_tag="PKO06_13045"
     CDS             complement(1173..1481)
                     /locus_tag="PKO06_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV70621.1"
                     /translation="MQPKNLKAFERRAIIASALAHPLRLQMLDWLYRRGSTCVCELVE
                     ACACSQPIASKHLLILKNAGLVEVRKDGLKAFYSLKTPCIMTFFECADRAFENHKKSL
                     "
     gene            complement(1621..>2780)
                     /locus_tag="PKO06_13050"
     CDS             complement(1621..>2780)
                     /locus_tag="PKO06_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70622.1"
                     /translation="VFAEVKRLWERGAKYVMLTDDHFSADLDRAKRISELITQAGIRI
                     AFFCFLRPFLGRMDVKRAMVQAGFVMLSYGAESPDPEQLKRFGKGFSDPVNFIRDVNR
                     EWHEAGARYIGNSFVFGDPRESRANIELLGDYARELDPTFIEPLYSQPFPGTRYRADL
                     EKAGQLRPHTWDEVTEARMLVKHPEYEEGELRNARVRMWLRFFSPRKVAGGFRVPLYL
                     IEQVGLPVRTVLKYMKACDYSVFGCILEEKFYLHRKRAMVREYLVEHVNSFSERERDM
                     TPFTEDFTSAIGLGWLKRRLGTRTFAVEIVDRGRLVTRFALALRDGRIAKMWAGAGCA
                     RDAAQEPTRFDHVWSVSLEDAIEIVCAHEAGKLAALVRTVVKNLVAVCLRG"
BASE COUNT          595 a          849 c          801 g          535 t
ORIGIN      
        1 atgcagcccc agaaagaagc agatggccgc gatcacgtat ttccatgagg gaccggtcag
       61 atacggcgcg aacgccgccg aaaccgagaa cagcaccgcc agttcgagcg caaaaccaaa
      121 cacgaaaaag aaactgaacc ccagcgcgcg cttccaggct ccatcgccct gcgcatatcc
      181 accgacgacg ccgataatca aaggcaccgt ggccaggacg cacgggttga gtgctgtcag
      241 gacaccgccg ccgaacgccg ctgccggagc taaccaggtc gaactcgcga tgacatcggc
      301 cagctgaagt tccagagcgc tgaggtccat cgattactcc ttgagcagaa cgacacccag
      361 gctctcgaac tgtttccgaa tgtcttccag cggcaggaag ccttcatggc gatagacttc
      421 cttgccgttt gcgtcgaaga agatctgcgt cggaatcatg cgaatgccgt aggtgctgcc
      481 ggcgtcacga tccttccaga catcgatgaa gatcacgtct gccttgcctt cgtagagcct
      541 tttcgcttct tcgagaaccg gttccatctt cttgcaggca ttgcacttgc cggcgccgag
      601 atcgaccatc ttcggcaacg gccctcgtac caccgccggt gttgccgtcg cggaaccagc
      661 ggctgattca ctggccacac ccgcgacctt gcccggatcc gaagccggct gtgtggtcgg
      721 cacgacggcg gaaggctgcg gagcagttgc cgatgcggtc aggggaactt caaccaccgg
      781 agcggattcg ctcgcctggg caaacgccgg cgaaccacag aggcaggatg aactgccctg
      841 cgataaatgc agcgccatca ggagtgtgac gacagcaacg acaatcagac ggggaagttg
      901 aatcatttcg gcgactcctg attctggcta tttaagaaag cccttgagtt cgtcaagcga
      961 cggaagtttg ccggagacct tgaccttgcc atcgaccgcc agagccgggg tgaccatgac
     1021 gccgaacttc atgatgtcgt tgagactggt gaccttctcg agctgatagt cgagcttgag
     1081 ttcgtctgca gcctgtttcg ccagttcagc cagtttcgtg catttggcgc agccggtacc
     1141 gagaatctga atcttcatgg atggtctcct ggttacagtg atttcttgtg gttctcgaag
     1201 gcgcggtcag cgcactcgaa gaacgtcatg atgcacggag tcttgaggga atagaacgcc
     1261 ttgagtccat cttttcgaac ctcgaccaac ccggcattct tgaggatcaa cagatgtttg
     1321 ctggcgatgg gctgactgca ggcgcacgcc tcgaccagct cacagacgca ggtcgagccg
     1381 cggcggtaca gccagtcgag catctgcagg cgcagcggat gagcgagagc cgaagcgatg
     1441 atcgcccgtc gctcgaaagc cttcagattt tttggttgca tgtattctta tataccgttt
     1501 ttggaatatt atgtcaagcc cttcgatagt tggggtcgaa atcgagggat cgaacacctg
     1561 cgccgacgca agccgcgttc cgcgcgcccg gacaaccttg gatgcggatc gtgagcgcgg
     1621 tcagccgcgc agacaaaccg ccacgagatt cttcaccacc gttcgcacca gcgcggccag
     1681 tttgcccgcc tcgtgcgcac agacgatctc aatcgcatcc tcgagagaca ccgaccagac
     1741 atgatcgaat cgcgttggtt cctgagccgc gtcccgggca cagccggcgc ccgcccacat
     1801 cttggcgatg cggccgtcac gcagggccag ggcaaaacgc gtgaccagcc ggccgcgatc
     1861 gacaatctcg acggcgaatg tccgagttcc gaggcggcgt ttcagccaac ccaggccaat
     1921 ggccgaggtg aaatcttcag tgaacggtgt catgtcgcgt tcacgctcgc tgaacgagtt
     1981 gacgtgctcg accagatact cgcgcaccat cgcccgcttg cgatgcaggt agaacttctc
     2041 ttcgagaata cagccgaaca ccgagtagtc acacgccttc atgtatttga gcaccgtgcg
     2101 caccggcagg ccgacttgct cgatcagata cagcggcacc cggaagcccc ccgcgacctt
     2161 gcgcggcgaa aaaaagcgca gccacatgcg cacccgcgcg tttcgcagtt ccccttcctc
     2221 atattccggg tgtttgacca gcatgcgagc ctcggtgacc tcgtcccagg tgtgcggccg
     2281 caactggcct gccttttcga gatcggcgcg ataccgtgtt cccggaaacg gttgtgagta
     2341 cagcggctcg atgaaagtcg ggtcgagctc gcgggcatag tcccccagca gttcaatgtt
     2401 cgcgcgcgac tcgcgcgggt caccgaagac gaatgagttg ccgatgtagc gggcgccggc
     2461 ctcgtgccac tcgcgattga cgtcacgaat gaagttgacc gggtcggaaa accccttgcc
     2521 aaaccgcttc agttgttccg ggtccggaga ttcggctccg tagctgagca tgacgaaacc
     2581 ggcttgtacc attgcccgtt tgacatccat gcggcccaga aacggtctga gaaagcagaa
     2641 aaaagcgatg cgaatcccgg cctgcgtgat cagttcagag atgcgcttcg cccggtcgag
     2701 atcggcggaa aagtgatcgt cggtcagcat gacgtatttc gcgccgcgct cccacagacg
     2761 cttcacttcg gcaaacacgt
//
LOCUS       DAORHD010000617         2776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_42837_length_2776_cov_3.252848, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000617 DAORHD010000000
VERSION     DAORHD010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42837_length_2776_cov_3.252848"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKO06_13055"
     CDS             <1..734
                     /locus_tag="PKO06_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV70623.1"
                     /translation="FVHLFPGSTRTTYREEVASLLASSGNPADFSPEEFVGAKLLWGI
                     GFGALSFTLFNFQSFGFWSLLMLVIWFIVGFSLPEVYLKRLIRKRQQNIEYELPYTID
                     LLSLSIEAGLDLIGGIQRIVEKSRPTDTVVEFQMFLSDIKLGKSLEEALSEMADRVQV
                     LSFFSFVSSLIQAQRLGAEIGPTLRAQAEQMRYQRMILAEEKVNQLPVKLLFPLVFFI
                     FPSIFVMVMGPSLIHLFKSLSTVAR"
     gene            751..1263
                     /locus_tag="PKO06_13060"
     CDS             751..1263
                     /locus_tag="PKO06_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HNV70624.1"
                     /translation="MRVLVSTCLLGIASTYVGRPNSAWSDGFEHVFPAAVAAGICFIP
                     VCPEQLGGLPTPRPPSELQAPALSVLQGNGRIVASTGTDVTAAFLRGADLTLQIATRF
                     DVRLAVLKTRSPSCGVGEVYSGRFDRCLVSGDGLTTALLRQHGLHVLSDREFFERWHR
                     VQNFSFLENL"
     gene            1688..2173
                     /locus_tag="PKO06_13065"
     CDS             1688..2173
                     /locus_tag="PKO06_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70625.1"
                     /translation="MLVVTGLGLGWHIYLGNPILCGFYCFLAPAILFTALFLFLGVLV
                     LTYRKCVLISKLQSCIEYMESSLFKTRRATFRFDQVSHIELCSVGECLFQTEACLFVV
                     KAYFRQEGVPHTFGVRLFESLDKTLAEDAAQTLSHIVRRPVVQQPALQLNPSFTSRVG
                     I"
     gene            complement(2253..>2776)
                     /locus_tag="PKO06_13070"
     CDS             complement(2253..>2776)
                     /locus_tag="PKO06_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70626.1"
                     /translation="AHRSFSFDGDRTMKLKDLCAMVCGVMAKKDKAADSERVQKLVAG
                     KNAEADATREDLAMMLGTLTGSISAENKIAPVVYTDVEGGSPLEEALTALSGQGITLG
                     YPNKVLRCAEPISWFEGVGVMAALGKAMNWFEPIVPDEVAGKSDFERLANVLKEKKAR
                     VRAILERSRSKRQ"
BASE COUNT          511 a          868 c          702 g          695 t
ORIGIN      
        1 cttttgttca tttgtttccg ggcagcaccc gcacgacgta ccgtgaagaa gtggcatcat
       61 tgctcgcctc gagcggcaat cccgccgact tttcgcccga agagtttgtc ggcgccaagc
      121 tgttgtgggg tatcggtttc ggcgcgctca gcttcacgtt gttcaacttt cagtcgttcg
      181 gtttctggag cctgctgatg ctggtgatct ggttcatcgt cgggttctca ttgcccgagg
      241 tgtatctcaa acgcctgatc cgcaaacgac agcagaacat cgagtatgag ctgccttaca
      301 cgatcgactt gctctctctc tcgattgaag cgggtctcga cctcatcggc ggcattcagc
      361 gcatcgtgga aaagtctcga ccgaccgaca ccgtagtcga attccagatg ttcctttccg
      421 acatcaagct cgggaaatcc ctggaagaag cgctttctga gatggctgac cgcgtgcagg
      481 ttctttcatt cttctcgttt gtcagctcac tgattcaggc tcagcgtctc ggcgccgaga
      541 tcgggcccac gctgcgcgcc caggcggaac agatgcggta tcagcgcatg attctcgccg
      601 aggagaaggt gaaccagctc ccggtcaaac tgctgtttcc gctggtattc ttcatcttcc
      661 cctcgatctt cgtcatggtc atgggtccgt ccctgatcca tctgttcaag agtctcagca
      721 cggtcgcccg ctgaggcgcc tcgacgccag atgcgcgttc tcgtcagcac ctgcctgctg
      781 ggtatcgcca gcacgtatgt gggccgaccg aacagcgcct ggtcagacgg ttttgaacac
      841 gtcttccccg ccgctgtcgc ggcggggatc tgctttatcc ctgtttgccc tgaacagctc
      901 ggcgggttgc cgaccccccg gccgccgtca gaactgcagg cccccgctct ttcggtgctg
      961 caggggaacg gccgaatcgt cgcttccacg ggaaccgacg tcacggcagc ttttctccgc
     1021 ggtgccgatc tgacgctgca gattgcgacc aggttcgacg ttcgtctggc cgtcttgaag
     1081 acgcgcagcc cgtcatgcgg cgtgggagag gtctactccg gccggtttga ccgttgcctg
     1141 gtctccggag atggactgac caccgctctt ctgcgacaac atggtcttca cgtcctcagc
     1201 gaccgcgagt ttttcgaacg ctggcacagg gtacagaact tttcttttct cgaaaatctc
     1261 tgaccatcgt cgtttcaacg tctctctcgg tgtacacctg tacacatttt catctcaacg
     1321 cgcctgaaat gataatcgtc attctgtaca cattcttttt tcttcgccga tatggtctgc
     1381 atctcatttc attcggtaca ggaaaaatat ttttactccc ttttcgttca cagtaccgga
     1441 gtcctgaatg tacgcctttt ttcaatagaa ttgctattga tttcacggtc aaccggtgct
     1501 agtatccggc ccacgaaaac gttttcgagt atcgaccaca gacgaaaacg aatcatcaga
     1561 ggcaacccac cacggagaat agagaataat gggattcctg agctttttga aagggatgaa
     1621 aattgaggac aactgcccca tcgacgttac cttccggacc tcgcgcaccg gaaaatgttt
     1681 tggctccatg ctcgtcgtca ccgggctcgg gctcggctgg cacatctatc tggggaaccc
     1741 cattctctgc ggtttttact gcttccttgc ccctgcgatt ctcttcaccg ctctgtttct
     1801 tttcctggga gtcctggttc tcacctaccg aaagtgcgtc ctgatcagca aactgcagtc
     1861 ctgcatcgag tacatggaat cgagcctgtt caagacgcga cgggccacct tccgtttcga
     1921 tcaggtgtcg cacattgaac tgtgcagtgt cggtgagtgc ctgttccaga ccgaagcctg
     1981 tctcttcgtg gttaaagcgt atttccgcca ggaaggcgtt ccgcatacct tcggagttcg
     2041 tctgtttgag agcctcgaca agacgctggc cgaagatgcc gctcaaaccc tttcgcatat
     2101 agtcagaagg ccggttgtcc agcaaccggc cctccaactc aatccgtcct tcacgagccg
     2161 ggtgggtatc tgatctcccg ttttccaccc attccctagt cgcccccggc cacggccggg
     2221 ggtctttttt tccccggcac gccgcaagct gcctactggc gcttggaccg gctgcgttca
     2281 agaattgccc gcacacgcgc cttcttttct ttcagcacgt ttgcgagccg ctcaaaatca
     2341 gattttccgg caacttcgtc tgggacgatc ggctcaaacc agttcatcgc ctttcccaga
     2401 gccgccatga ccccgacacc ttcaaaccac gagatcggtt cggcgcagcg caataccttg
     2461 ttcggatacc ccagcgtgat gccctggccg ctcagcgccg tcagcgcttc ctcgagcggc
     2521 gatccacctt cgacatccgt atacaccacg ggcgcgatct tgttctcggc actgatcgat
     2581 ccggtcagcg ttcccagcat catggccaga tcttcacgcg tggcatcggc ttccgcgttc
     2641 ttgccggcaa ccagcttctg aacccgctca gaatcggccg ctttgtcctt cttcgccatg
     2701 accccgcaca ccatggcgca caggtctttc agcttcatgg tgcgatcacc gtcgaaactg
     2761 aaagacctgt gcgcca
//
LOCUS       DAORHD010000618         2765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43107_length_2765_cov_2.891513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000618 DAORHD010000000
VERSION     DAORHD010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43107_length_2765_cov_2.891513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1130
                     /locus_tag="PKO06_13075"
     CDS             165..1130
                     /locus_tag="PKO06_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV70627.1"
                     /translation="MMRKNFVLIGAGGYIAPRHLQAIKDTGNNLVAALDRSDSVGILD
                     RYFFDVDFFQEFERFDRHVDKLRRLGDDKRIHYVSICSPNYLHDSHIRFALRNGAHAI
                     CEKPLVLNPWNLDALAQLEQETGQRVYNILQLRLHPSIVGLRERVLAMKQKTFDVDLT
                     YITSRGKWYFYSWKGALEKSGGIATNIGIHFFDMLIWIFGAVKRSEVHVSEASKAAGY
                     LELERARVRWFLSIDRNDLPHSAAIKGKTTFRSLTMNGEEIEFSEGFGDLHTKVYQEI
                     LAGRGFGIEDARASITLAHQIRNAAVSSDQSQNCHVCVLPKPIQQ"
     gene            1170..>2765
                     /gene="asnB"
                     /locus_tag="PKO06_13080"
     CDS             1170..>2765
                     /gene="asnB"
                     /locus_tag="PKO06_13080"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV70628.1"
                     /translation="MCGITGVFSLNEDVAPRYIAEMTDLIRHRGPDDEGYLAVWTPGK
                     RVQALSGRDSKVPLPSLSDRQMQANLYLGHRRLSIIDTSSAGHQPMCSSDGKHWLVYN
                     GEIYNFRELRRELLDRGYRFVSNTDTEVLLAGYSCWGTDLPKLLNGMWAFVIYDREKN
                     VLVGSRDYAGVKPIYYVHEGDRFAFASEAKSLICLPWVSRKVNPTAVACYLVEGLDQS
                     AYGGFFEKVRELGPGYQFSLDLTNNKLTQSRYFQLEWTSASPSVSVREARQIVETVRE
                     NVISAVERHLMSDVKVGSCLSGGIDSSSIVGVIRALQRQNELVQIGSRQLVLTACYPD
                     SSIDESHWAEKVAREAGAEWIRTYPTAQGMLESLEDLVYTQDFPFASSSIYAQYCVMK
                     LAREHGISVLLDGQGGDELFTGYPPYYTVYWAELLKQGHWRDIRRELRFLSTADTTVS
                     QLVGSLVKYAGSRCLPSIRRSMVQKQFRHFPWISTELHGHLQQPITEGAPFGNLNAEL
                     AHLFQGGNLQSLLRYEDRNSMRFS"
BASE COUNT          612 a          702 c          784 g          667 t
ORIGIN      
        1 gtgttttttc tggtcgggtt ctatgccagt ctcgtcgtcg tgaaatgcac ggacgaagag
       61 gtgggctgat ggggccagga gattgcgcgg cttctgcgtc gaagttttcc ttgcgcacgt
      121 tctgcagtcg ggtgtatcta tgacaccccc attgaggtga tggaatgatg agaaagaact
      181 tcgttctgat cggagccggt ggatacatcg cgccgcgaca tctgcaggcc atcaaagata
      241 cgggaaacaa cctggtggct gcgcttgatc gaagcgactc ggtcgggatc cttgaccggt
      301 acttttttga cgtagacttc tttcaggagt tcgagcgctt cgatcgtcat gtcgacaaac
      361 tgcgccggct tggcgacgat aagcgcattc attacgtgag catctgctcc cccaactacc
      421 ttcacgactc gcacattcga tttgccctgc gaaacggagc gcatgcaatc tgcgaaaaac
      481 cgctggttct gaatccctgg aacctggatg ctctggcaca attggaacag gaaaccggac
      541 agcgtgtata caacatcctg cagttgcgcc tgcacccctc gatcgtcggt ttgcgcgaac
      601 gcgtcttggc gatgaaacag aagacatttg acgttgatct cacgtatatc acgtctcgag
      661 gaaagtggta cttttactca tggaaaggtg cgctcgaaaa atcgggtggc attgccacga
      721 acatcggcat tcactttttc gacatgctca tctggatctt tggcgcggtc aaacggagtg
      781 aagtccatgt ctctgaagcg tcgaaagcgg cggggtatct ggaactcgag cgcgctcgtg
      841 ttcgctggtt cctgtctatc gaccggaacg atcttccgca ctcagcagcg atcaagggga
      901 agacgacgtt tcgttctctc accatgaacg gcgaagaaat cgagttctct gaaggttttg
      961 gcgacctgca caccaaagtg taccaggaga tcctggccgg ccgcggtttc ggcatcgaag
     1021 acgctcgcgc ctccatcaca ctcgctcacc agattcgcaa tgcagcggta agcagcgatc
     1081 agagtcagaa ctgccacgtg tgcgtcttac cgaaaccgat tcaacagtga tgtcactccg
     1141 gcatgttcct gtaagacgaa tctcctgaca tgtgcggcat cacgggagtt ttttcgctga
     1201 atgaagatgt agcgccgcgc tatatcgccg aaatgacgga tctcatccga catcgggggc
     1261 ccgatgatga gggctacctc gccgtttgga cgcccgggaa acgggtacaa gctctcagcg
     1321 ggagagactc taaggttccg cttccgtctc tctccgacag acaaatgcag gcaaacctct
     1381 atcttggaca tcgccgcctg tcgatcatcg acacgtcttc ggccggtcac caacccatgt
     1441 gtagttccga tggcaagcat tggctggtct acaacggcga aatctacaat ttccgtgaac
     1501 ttcgcaggga gctcctggac aggggctacc ggtttgtcag caatacggat accgaagtgc
     1561 ttctggccgg gtactcctgc tggggaaccg acctgccaaa gctgctgaat ggaatgtggg
     1621 ctttcgtcat ctatgaccga gagaaaaacg tcctggttgg atcacgagat tatgcaggcg
     1681 tcaagccaat ttactatgta catgaaggtg acaggtttgc gttcgcttcc gaggcgaagt
     1741 ccctgatctg cctcccgtgg gtttcccgaa aggtgaatcc cacggcggtc gcttgttacc
     1801 tggtggaggg gctggatcaa agtgcatacg ggggcttttt tgagaaggtt cgcgaattag
     1861 gtcccggata ccagttttcc cttgatctca caaacaacaa gttgacacag tctcgctact
     1921 tccagttgga atggacgagt gcgtctccct ctgtctctgt gcgtgaagcc agacagattg
     1981 tagagacagt cagagaaaac gtgatttctg ctgtggagcg gcatctcatg tcggatgtca
     2041 aagtggggag ttgtctgtcc ggtggaatcg atagttcatc gatagttgga gtcatacgtg
     2101 ctcttcagcg acagaatgaa cttgtacaaa ttggaagtcg acaacttgtt ttgaccgctt
     2161 gttatccaga ttcttcgatt gatgaaagtc actgggccga gaaagttgca cgagaggcgg
     2221 gggctgagtg gatccgcacg tatccgacgg ctcaaggaat gctggagtcg cttgaggatc
     2281 tcgtctacac gcaggatttt ccgtttgcct cgagcagtat ctacgcgcag tactgtgtca
     2341 tgaaactcgc cagggagcat ggtatttcag ttctgctgga tgggcaagga ggcgatgagc
     2401 ttttcacggg atatccgccg tattacaccg tctactgggc cgagttgttg aagcagggcc
     2461 attggcgcga tatccgccgg gaactgcgtt ttttgtcgac agccgatacc acggtctccc
     2521 agttggtggg cagtctggta aagtatgcgg gaagtcgctg tttgccgagt attcggagaa
     2581 gcatggttca gaaacaattc cgccatttcc cctggatttc aacggagctt catggacatc
     2641 tgcagcaacc gatcactgaa ggggccccgt tcggaaatct caatgcggag cttgcacatt
     2701 tgttccaggg ggggaacctg caatcgctgc tgcggtatga ggaccgcaat tcgatgcggt
     2761 tctcc
//
LOCUS       DAORHD010000619         2764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43124_length_2764_cov_3.007752, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000619 DAORHD010000000
VERSION     DAORHD010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43124_length_2764_cov_3.007752"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PKO06_13085"
     CDS             <1..849
                     /locus_tag="PKO06_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV70629.1"
                     /translation="AVKTLGIVQGTYSGFVQGQKDQKKAVWGFSAYATAANSVEFNLV
                     DGAGATAAITAYTTVTDVTASDMIRFTTFQASVNTLLENATVSIRIDQVGSTVAFTSL
                     SVGQENPDGQAAIRSMVSFTGDDPFNQMFGLSNGTAMGSGDKNFRVHVVDNTPKFQIG
                     GDPGQSMAVGFASMNAEALGVQSLDMSSISGAERSLAKVNSAIDKVSAERSKLGAFQN
                     RLEYAINNLRNTYSNLASAESRIRDADIALEMIEFTRDQIVNQSGTAMLAQANLLPQG
                     VLQLLR"
     gene            1267..>2764
                     /locus_tag="PKO06_13090"
     CDS             1267..>2764
                     /locus_tag="PKO06_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70630.1"
                     /translation="MRINQNVLAIRTHGQIAATNTRTDKSIEKLSSGLRINRAADDAA
                     GLAISEKLRRQVRGLSRAILNAQDGISMIQAAEGSLNESHSILQRMRELAIQSSNDTL
                     TSNDRLEIQKEVVQLRDDLNRIARNTEFNTKKLLDGSQTALISASSNSVQGFVTGSGA
                     AGGDVNISLALITGGISQMNRSQIFSVKGQESSLALGTTQLQSIAQFYDANGVFQLQS
                     SQQLTLIGNSKTTSITLDGQMTLDKLAGAIQNALSTSSGLDLANSKVAIVNTAVTQIS
                     GLGGYLQIVSGAVGEEGKIAFAGDQGLLNALGITETRAAKNNMVEATLRDGNGNMRQI
                     RTDNSRIVGLLDGIDLEFTSQAAQVAGTQGLEQGLLITAGGAQLFTINAGGTITVQVS
                     VSSGFWTMEGIARSINRQIELQVTAALYGANAQSLQDGLKAMVVDGQIRIGWEAPSNV
                     VSATGTMKIVGASAAETIGLLNGTYSGFVQGTRDVEKVEWGFSQYIDTA"
BASE COUNT          661 a          696 c          830 g          577 t
ORIGIN      
        1 gccgtgaaga ctctgggaat cgtgcagggc acgtacagcg gatttgtcca ggggcagaag
       61 gatcagaaga aagctgtctg gggtttctca gcctatgcaa cggcggcaaa ctccgtggag
      121 ttcaatctgg tcgatggcgc cggggccacg gcagcgatta ccgcctatac cacggtgacg
      181 gacgtgacgg cctcagacat gattcgattc acgaccttcc aggcaagcgt caacacgctg
      241 cttgagaacg cgacggtatc gatcagaatc gatcaggtgg gttcaacggt cgccttcacg
      301 tctctcagtg tgggacagga gaatccggat ggtcaggcag ccattcgcag catggtctcg
      361 ttcaccggcg atgatccgtt caatcagatg ttcgggttgt cgaatggaac ggcaatggga
      421 agcggcgaca agaactttcg ggttcatgtt gtcgataaca cgccgaagtt ccagatcggt
      481 ggcgatccgg ggcagtctat ggccgtgggc tttgccagca tgaatgcgga agcattgggc
      541 gtgcagagcc tggatatgtc atcgatcagt ggggctgaac gatctctggc caaggtgaat
      601 tcggcaatcg acaaagtgtc cgcggagcgc tcgaagttgg gggctttcca gaatcgtctg
      661 gaatacgcca tcaacaacct gcgcaacact tacagcaacc tggcctctgc cgagtcgcgc
      721 atacgtgacg cggacattgc gctggagatg attgagttca ccagagatca gatcgtgaac
      781 cagtcgggaa cggcaatgct ggctcaggca aatctgcttc cacagggagt tctgcagttg
      841 ctgagatagg actatcgcaa taactctgga taacggttca gagacaacaa ggccgatcga
      901 tcttgatcgg tctttctttt tttggcccat ttgcagtcga ctcggaggaa tgaagcgaaa
      961 acgggcaaaa acaagccggt tagtagagaa agcgcagaaa atagagacgc catggattgc
     1021 tagctggtct acaggttgaa gatcgacctg tacggaaatc tggcctggaa gacgatctaa
     1081 acgatagccg atagagggga tgttcttgcc tgttctcctc tgtccgaaaa aatacagttc
     1141 ggaggtagcc tagggtagac ggttccattt gagttcccat aaagctctgt cagtacaact
     1201 tcatctatca atcattggcg ctaccgaagc agggatgctt cgggaacaga ttcaaggagg
     1261 aatcatatgc gtatcaatca aaacgttctc gcgattcgga cccatggaca gattgcggcg
     1321 actaacactc gcacggacaa gtcgattgaa aaactgagtt cggggttacg gatcaatcgc
     1381 gcggctgatg atgctgctgg cctggcgatc agtgaaaaac tgcgccggca ggttcggggt
     1441 ttgtcccggg cgattctcaa cgctcaggac gggatctcca tgattcaggc ggctgaaggc
     1501 tccctgaatg aatcacacag catcctgcag cgtatgcggg aactggctat ccaatccagc
     1561 aacgatacgt tgaccagcaa cgatcggctc gagattcaga aggaggtggt ccagcttcgg
     1621 gatgatctga accggattgc ccgaaacacc gaattcaata ccaaaaaatt actcgatggg
     1681 tctcagacgg cactcatcag tgccagttcg aactcggttc agggctttgt gaccggctct
     1741 ggtgccgcgg gtggtgacgt caacataagc ttggcgctca ttacaggcgg tatctcacaa
     1801 atgaaccgca gccagatctt ctcggtcaaa ggtcaggaat cgtcactggc gctgggcacg
     1861 acgcagctgc agagcattgc gcagttctat gatgccaacg gtgtctttca gttgcagagc
     1921 tcgcagcagt tgaccctgat cggcaacagc aagaccacgt cgatcacgct ggacggccag
     1981 atgacactcg ataagctggc cggcgccatt cagaacgcgt tgtccacgag ttcgggtctg
     2041 gaccttgcca actcgaaggt ggcgattgtc aacacggcag tcacgcagat atcaggcctt
     2101 ggcggttatc ttcagatcgt ctctggtgcc gtcggtgaag aaggcaagat cgccttcgcc
     2161 ggcgaccagg ggctgctgaa tgctcttggc atcacggaaa cccgcgcagc caagaacaac
     2221 atggtcgagg cgacgttgcg cgacggcaat ggcaacatgc gccagattcg cactgacaac
     2281 tcgcgtatcg ttggtctgct cgacggcatc gatctggaat tcacctcgca ggcagcacag
     2341 gttgccggaa cccaggggtt ggagcagggc ctgctgatca cggcgggcgg cgcgcagctg
     2401 ttcacgatca atgccggtgg cacgatcacg gtacaggttt ctgtcagcag cgggttctgg
     2461 acgatggaag ggatcgctcg gagtatcaat cgccagatcg agctccaggt gaccgcagcg
     2521 ttgtatggcg cgaatgctca gtcgttgcag gacggactga aagccatggt ggtcgatggc
     2581 cagattcgca tcggctggga agctccaagc aatgtcgtga gtgctacggg aacgatgaag
     2641 attgtcggtg cgtcggcagc agaaacaatt ggccttctga acggtaccta cagtggattt
     2701 gtgcagggaa cccgtgatgt cgaaaaggtg gaatggggat tctcgcaata cattgatacc
     2761 gccc
//
LOCUS       DAORHD010000620         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43293_length_2757_cov_3.763509, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000620 DAORHD010000000
VERSION     DAORHD010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43293_length_2757_cov_3.763509"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..>2757)
                     /locus_tag="PKO06_13095"
     CDS             complement(23..>2757)
                     /locus_tag="PKO06_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV70631.1"
                     /translation="FQLFLSLLDSDRFYGKFRDGAHKVDVSDLMRRMVKEDLLTFQGT
                     KLFPERRAITANYTLSDLEEALYAAVTEYVRTEMNRADRIEDGRRRGNVGFALTALQR
                     RLASSPEAIYQSLKRRRNRLIRQLEEAKLLRRGSIVAETCAPYMAEDVDDPDDLNAEQ
                     TEELEEKLVDLATAAETIAELEAEIQTLTRLEAQACEVVHSEQDRKWEELSNLLQNTP
                     EMHDREGRQRKLIIFTEHRDTLNYLTVKIGGLLGDAKAVINIHGGTKREDRRKAQELF
                     RNDPDIRILVATDAAGEGVNLQNANLMVNYDLPWNPNRIEQRFGRIHRIGQTEVCFLW
                     NLVANQTREGDVFQRLFEKLEVERKALGGRVFDILGDVFNEKSLRELLIEAIRYGEDP
                     ARKQALLNKVDGALDSEHLRNIISNNALCEEVMDEQRLFAVKAAMEKAEARKLQPYFV
                     RAFFEEAFRHLGGELKPRETGRWSISHVPAIIRERDRQISGRDRRFLAPVVTKYDRVC
                     FEKKNVRLLDRVGAPPAELLHPAHPLMQAMVDLVLEQHRTKLKQGTVFLDPNDTGTTP
                     RLLFIIEHAVREGADPSRTVSQRMQFVEILPDGRAVNAGYGPHLDLEPLSDADRRLIE
                     DLLDQPWIKRDLEQQALAFTSEKLVPEHFTEVRDRREHQIDKTMTAVKERLVKEINFW
                     STRQISLEEDIAAGKEVRLQLDNVKRTIEELTSRLQSREQDLAKQRHVISATPVVIGG
                     ALIIPAGLLVTRQGQTTPTTETGNTPDQPDALDRQTVEKIAMNAVIQAELAMGHQVID
                     VSAEKCGWDVTSIPPARDGRLPTVRHIEVKGRAKGADTITVTRNEVLYGFNQGDKFML
                     AIVLVGPDQSADGPYYLSRPFDREPGWAETSINFDLAQLLTRAQKPLLVAQTTP"
BASE COUNT          482 a          768 c          885 g          622 t
ORIGIN      
        1 aaggataata gcactttcgt tattaagggg tggtttgagc gaccaacagg ggtttctgag
       61 cgcgagtcag caactgggcc aggtcgaagt tgatgctggt ttcggcccag ccgggctcgc
      121 ggtcaaaggg ccgtgagagg taatacggac cgtcagccga ctggtcaggg ccgaccagca
      181 cgatggccag catgaacttg tcgccctggt tgaagccgta gagcacctcg tttcgggtga
      241 cggtaatggt atctgcgccc ttggcacgcc ccttcacctc gatgtgccgg accgtcggaa
      301 gcctgccgtc tcgggcgggt ggaatactcg tgacatccca gccacacttc tcggctgaca
      361 catcgatgac ctgatgcccc atggccagct cggcctggat gactgcgttc atggcgatct
      421 tctcgacggt ttgccgatca agcgcatcgg gttgatcagg cgtgttgccg gtctcggttg
      481 tcggcgtcgt ctgaccctgt cgcgtgacca gcaaccctgc cggaatgatc aatgccccgc
      541 cgatgaccac gggggtggca gatatcacgt gccgctgttt ggcgagatcc tgttcgcggc
      601 tctgcaggcg tgaggtcagt tcctcgatgg tgcgcttcac gttgtcgagc tggagcctga
      661 cctcttttcc agcggcgata tcctcttcga gcgagatctg gcgcgtggac cagaagttga
      721 tttccttcac cagccgctct ttgacggcgg tcatcgtctt gtcgatctgg tgttcgcgac
      781 ggtcacgaac ctcggtgaag tgctcgggaa ccagtttttc ggaggtgaag gccagggcct
      841 gctgttcgag gtcgcgtttg atccagggct ggtcgagcag gtcttcgatg aggcggcgat
      901 ctgcatcaga aaggggttca agatcgagat gcgggccata cccggcattg acggccctgc
      961 cgtcgggcag gatttcgacg aactgcatac gttgagaaac ggtgcgactg gggtctgccc
     1021 cttcgcgaac cgcatgttcg atgatgaaga ggagacgtgg cgtcgtgccg gtatcattgg
     1081 ggtcgaggaa aaccgtgccc tgcttgagct tggtgcggtg ctgttccaga accaggtcga
     1141 ccatggcctg catgagtggg tgagccgggt gtaacagttc ggcaggcggg gctccgacgc
     1201 ggtcaagcag tcgcacgttc tttttctcga agcagacgcg gtcatatttg gtgaccaccg
     1261 gtgccagaaa tcgcctgtct cggccactga tctgccggtc acgttcacga atgatggcgg
     1321 gcacgtgaga gatgctccag cggcccgtct cacggggttt gagctcaccg cccaggtggc
     1381 ggaacgcttc ctcaaagaag gcccggacga aatacggctg gagcttgcgg gcttctgcct
     1441 tttccatggc ggctttcacg gcgaacaggc gctgttcgtc catgacttct tcacagagcg
     1501 cgttgttgga aatgatattg cgcaggtgct ctgaatcgag agctccgtcg accttgttca
     1561 gcagggcctg cttacgggcc gggtcttcac cgtatctgat cgcctcgatc agcagttcgc
     1621 gcagactctt ttcgttgaag acgtctccca gaatgtcgaa cacgcgacca cccagcgctt
     1681 tccgctccac ttcgagtttc tcgaacaggc gctgaaacac gtcgccctcg cgggtctggt
     1741 tggcaacgag gttccagagg aagcagactt cggtctggcc gatacggtgg atgcggccaa
     1801 agcgctgttc gatgcggttg gggttccagg gcaggtcgta gttgaccatc aggttggcgt
     1861 tctgcaggtt gacgccttca ccggcggcgt cggtggccac cagaatgcga atgtccgggt
     1921 cgttgcggaa gagttcctgc gccttgcgcc gatcttcgcg cttcgttcca ccgtgaatgt
     1981 tgatgacggc tttggcatcg ccgagcaggc cgccgatctt caccgtcagg taattcaggg
     2041 tatcgcgatg ctcggtgaag atgatcagct tgcgttggcg accttcccgg tcatgcatct
     2101 cgggcgtgtt ctgaagcaga ttcgacagtt cctcccactt gcggtcctgc tcggaatgca
     2161 cgacttcaca cgcttgagct tcgagacggg tcagcgtctg aatctcggct tcgagttcgg
     2221 caatagtctc tgcggcagtc gccaggtcga ccagcttttc ttcgagttcc tcggtctgtt
     2281 cggcgttgag atcgtcgggg tcatcgacat cttcggccat gtagggcgcg caggtctcgg
     2341 cgacgatact gccgcgacgc agaagtttgg cttcctcaag ctggcggatc aggcggttgc
     2401 gacggcgctt gagcgactgg tagatcgcct cggggctgga ggccagacgc cgttgcaggg
     2461 cggtcagcgc gaagccgacg ttgcctcgac ggcgcccatc ttcgatgcgg tcagcccgat
     2521 tcatctcggt gcggacatac tcggtgacag ctgcatacag ggcttcttcg agatctgaga
     2581 gtgtgtagtt tgcggtgatg gcgcggcgtt ccggaaacag cttggttccc tggaaggtca
     2641 gcagatcttc tttcaccatg cgccgcatga ggtcggaaac gtcgaccttg tgcgctccgt
     2701 cgcggaactt gccgtaaaag cgatcagagt cgagcagcga caggaagagc tgaaagt
//
LOCUS       DAORHD010000621         2753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43368_length_2753_cov_3.890289, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000621 DAORHD010000000
VERSION     DAORHD010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43368_length_2753_cov_3.890289"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1834
                     /locus_tag="PKO06_13100"
     CDS             <1..1834
                     /locus_tag="PKO06_13100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV70632.1"
                     /translation="LYGWPWYMGGQLLFANRGMCASAGVTLPADGRLTLEEFRDLLVK
                     LKGHQQGQTGAYPFGVYFHKDQTANFPFAFPPGCGWLEDDLKKMTDASLLSRQAEVMP
                     ASGLDWMLGLIRDGLMPSDTGGRNSNDTWIAFAREHRLGFGAFGLWAVKALTDKFPMD
                     FEILHFPAPKGQINPAYVGVSGFYVFRRGDESRTAAAMKLARFLTNADSQRAVKPYTQ
                     FPTRVSAGNIYEGDRIMTRAWEVVREGRTVPVDSRWPQVDEEIEAAVQETLLGRLKPQ
                     EALIQASERIGVLLGRESGAVHEELRKGSWIGKLFLYAFPIVLIFALLTRQVHLFFLI
                     PSFTILALFLFYPLLDAILLAFRDFKFGEVGGYTLQNFARAWGDPKFRMAAWNTGLYT
                     LIVVPANVLTALIASSLIFHLHGRWKGFFRAMYYLPGVASVVVLAMVWRYLFNTELGL
                     FNNVLRAIGLEPIGWLTDPNVAFISVMLTGILRSPGGAILIYLASLANVPKSLYEMAD
                     LEGASKIRQWWAITVPLMRHTTMFLLITGAIDALQVFAQVLMLTDGGPGISTAVVVHR
                     IYTAAFRDFDFGLSSAMALMLFVVIFLVTVAQRRWGASESMELA"
     gene            1831..2646
                     /locus_tag="PKO06_13105"
     CDS             1831..2646
                     /locus_tag="PKO06_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV70633.1"
                     /translation="MTRRSQHIFYLTVAVLAVGLILTVGPLIWMLITAFSDPAGLSEK
                     PMRYAFSLENFTTLLASGKLLRWAWNSLFTAGLITLGQMVFNSMAAFSFSVGRFRGRE
                     WLFTVILGAMMVPGQIVMLPLFLFLAKWNMIDNLWAVILPALASPFGIYMVRQYMDSL
                     PRELLEAARMDGATEVQIFHRIYFPLSMPILATSGIFIFITNWNAFLWPLVTLNTDLN
                     YTLTVGLSTLQDQQIMDYGLLMAGATLAALPMVVVFLIFSRYMLEGMRGGAIK"
BASE COUNT          451 a          782 c          866 g          654 t
ORIGIN      
        1 gttgtacgga tggccgtggt acatgggcgg tcagttgctg ttcgcgaatc gggggatgtg
       61 cgcgtcggcc ggggtcacgt taccggcgga tggtcggctg accctcgaag agtttcgcga
      121 tctgctggtg aagctgaaag gccatcagca ggggcagacg ggggcttacc cgtttggtgt
      181 ctactttcac aaagaccaga ccgcgaactt tccgttcgcg tttcccccgg gatgtggctg
      241 gctcgaagac gacctgaaga agatgaccga tgcgagtctg ctgagtcggc aggctgaagt
      301 gatgccggcg agcggtctcg actggatgct tggtctgatc cgcgatggac tcatgccgtc
      361 cgataccggt ggaagaaatt cgaatgatac ctggatcgcc ttcgctcggg aacatcgtct
      421 gggcttcggc gcattcggac tctgggccgt caaggcattg accgacaagt ttcccatgga
      481 ttttgagatt ctgcattttc ccgcgccgaa agggcagatc aacccggcgt atgtcggtgt
      541 ctcggggttc tacgtgtttc gacgaggcga cgagtcgcgt accgcggcgg cgatgaaact
      601 ggcgcgcttt ctcaccaacg ccgacagcca gcgggcggtc aagccgtata cgcagttccc
      661 gacgcgggtt tcggccggga acatctacga aggcgatcgc atcatgaccc gcgcctggga
      721 agtcgttcgc gagggtcgta cggtaccagt cgattcacgt tggccgcagg tcgacgagga
      781 gatcgaagcc gcggtgcagg agacgctgct cggtcggctc aagccgcagg aagcgctgat
      841 acaggcgagc gaacgcatcg gcgtgctgct ggggcgcgag tcaggggcgg tgcacgagga
      901 actgcgtaaa ggttcctgga tcggcaaact ctttctctat gcgtttccga tcgtgctgat
      961 cttcgcgctg ctgacgcggc aggtgcatct gttctttctg attccgtcgt ttacgattct
     1021 ggcgctgttc ctgttctacc cgctgctcga cgcgatactg ctggcatttc gagacttcaa
     1081 gttcggcgag gtcggtggct atacgttgca gaactttgcc cgcgcgtggg gtgaccccaa
     1141 gtttcgcatg gcagcctgga acacggggct gtacacgctg atcgtggtgc ccgccaacgt
     1201 gctgaccgcc ctgatcgcct cgagtctgat cttccacctg cacggtcgct ggaagggctt
     1261 cttccgggcg atgtactacc tgccgggggt cgcttccgtg gtcgttctgg ccatggtctg
     1321 gcgctatctg ttcaataccg aactgggact tttcaacaat gtcctgcgag cgatcggtct
     1381 cgaacccatc ggctggctga ccgacccgaa tgtcgccttt atctcggtca tgctgaccgg
     1441 cattctgcgg tcaccgggcg gcgcgattct gatttatctc gccagtctgg ccaacgtacc
     1501 gaagagcctc tacgaaatgg ccgatctgga aggggcctcg aagattcgac agtggtgggc
     1561 gatcacggtg ccgctgatgc gtcatacgac catgttcctg ctgatcaccg gggcgatcga
     1621 tgccctgcag gtcttcgcgc aggtattgat gctgaccgat ggcggccctg gcatttcgac
     1681 cgccgtcgtg gtgcatcgta tctacaccgc agcctttcgt gattttgact tcggtctctc
     1741 atcggccatg gcattgatgc tgtttgtcgt catcttcctg gtgacggtgg cgcagcgtcg
     1801 ctggggagct tcggaaagca tggaactcgc atgacacgtc gatcacagca tatcttctat
     1861 ctgacggttg ccgttctggc ggtggggttg atcctgacgg ttggcccgct catctggatg
     1921 ctcatcaccg ctttctctga tccggcgggg ctgtccgaga aaccgatgcg gtatgcattt
     1981 tcgctcgaga acttcacgac gttgctggcc tcgggcaagc tgttgcgctg ggcctggaac
     2041 tccctgttca ctgccgggtt gatcaccctc gggcagatgg ttttcaactc gatggccgcg
     2101 ttcagctttt ccgttggtcg attccgtggg cgggagtggt tgttcaccgt cattctcggc
     2161 gcgatgatgg ttcccgggca gatcgtcatg ctgccgctgt ttctgtttct ggcgaagtgg
     2221 aacatgatcg acaacctctg ggcggtcatt ctgccggcgc tggcctcgcc cttcggcatc
     2281 tacatggtgc gtcagtacat ggattcgctg ccgcgcgagc tgcttgaagc ggcgcgcatg
     2341 gacggcgcga ccgaggtgca gatctttcat cgcatctact tcccgctgtc gatgccgatt
     2401 ctggcgactt caggcatctt catcttcatc accaactgga acgcgtttct ctggcctctg
     2461 gtgacgctga acaccgatct gaactatacg ttgacggtcg gtctctcaac cctgcaggat
     2521 cagcagatca tggattacgg cctgctgatg gccggcgcga cgctggccgc gctgccgatg
     2581 gtcgtcgtct ttctgatctt ctcgcgctat atgctcgaag gcatgcgcgg tggagccatc
     2641 aagtgaccgc cgcccgcctg ctggtttcgc tcgactgggg tggtaccaag gtggccggag
     2701 tcgtggtcga tcttgacgcg ggtacgagcc gaaaattcgt ggttccggca acc
//
LOCUS       DAORHD010000622         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43466_length_2749_cov_3.429102, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000622 DAORHD010000000
VERSION     DAORHD010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43466_length_2749_cov_3.429102"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..1967)
                     /locus_tag="PKO06_13110"
     CDS             complement(288..1967)
                     /locus_tag="PKO06_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HNV70634.1"
                     /translation="MGEYPLVFIFTLGFVFLSILITYLTLNKGQSTSEFYVAGNQVPW
                     VQVGIAMVGSYLSAASFLGVAGDIAIKGVDRIWLAIGFFGGYMALLMLIAGPLRNVGS
                     YTVADALYRRFPDDRIKLIVMVTTLVISTFYLVPQVLGAGLVFELLLGWNFLVTTIGI
                     GVLISLYIIYGGMKATLYNQVVQAIFLWAAMVVIVIAGVFMFFDGSINKIFETAGQIV
                     PPSIAGPNAGAVAASAAAVSAGAAIDPALAAKYGTVIPASATAAFTAVRASMPDAATA
                     MTIGVQTPSTFAQISTVLALVFGTAGLPHILIMFFTVPSASAAKKSVTLCVIALGIFY
                     FCSIFLGFITLILVYPQLIAWIGAGKVGMATNMAVLKVAEMIGGQWLMAISTAGAVAA
                     ILSTAAGLMITCASTISHDFYKVYINKEATEAQEINIAKLTTVIMSAIAVLLAYVLKG
                     ENVAWLVTLAFGIAASAIFPTMIATLWWKKFTRQAALAGMSTGLGISVIFIVLLLSGV
                     KEVFGLSVVGGPGIFGVTVGILVVVLVSLFTNDYGKDVNGFFALAHKTEKE"
     gene            complement(1971..2237)
                     /locus_tag="PKO06_13115"
     CDS             complement(1971..2237)
                     /locus_tag="PKO06_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70635.1"
                     /translation="MVDAAPETVNPSPLDTEAEENYRRRMKVGFVLFAVFFAFYMWVA
                     IINTPPYREFAAMPCMGMPLGMFLSLAVFPVSWLILVVYFLVWR"
     gene            2458..>2749
                     /locus_tag="PKO06_13120"
     CDS             2458..>2749
                     /locus_tag="PKO06_13120"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV70636.1"
                     /translation="MKSFTFQAIVSVVLALLIFFLAVFLKILPAFERAILEQKREMIR
                     ELTNSAWNILARFETDERAGRLTRDEAQKQAVQQIRNLHYGREMKDYFWINDF"
BASE COUNT          656 a          753 c          828 g          512 t
ORIGIN      
        1 atgatcgttg cgaaagccgc aacaggacgg aaaacctctc cttgtgcatc tcgtctgccg
       61 ggggaaatgg ttctctggtg tgttcggcag gcgatgaacg gacatcgaag tgtagcgcac
      121 gaaagaaacg cgatgcaacg aaaagtttca tgcgtcggtg cgtgggcagc cgggattgga
      181 aggtaaaggg atcgtggcga gagccggtgc gagtcttcaa cggacgaccg aaccagcaaa
      241 aagggtggcc gcatgacgag catacggcca ccctttttgc gagaaggtta ttctttctcg
      301 gtcttgtggg cgagggcgaa gaagccgttg acatccttgc cgtaatcgtt cgtgaacaga
      361 ctgacgagaa cgacgaccag aatgccgacg gtgacgccga agatgccggg accgccgacg
      421 accgacagac cgaatacttc cttcactccg ctcagcagaa gcacgatgaa gatgaccgag
      481 atgccaagac cggtagacat ccccgctaga gccgcctgcc gagtgaactt cttccaccag
      541 agcgtggcga tcatggtcgg aaagatggca ctggccgcga tgccgaaggc cagggtcacc
      601 agccaggcga cgttttcacc cttcagcacg taggccagca gaacggcgat ggccgacatg
      661 atcacggtgg tcagcttggc gatgttgatt tcctgggctt cggtggcctc tttgttgatg
      721 tagaccttgt agaagtcgtg gctgatggtc gaagcgcagg tgatcatcag gccagcggcg
      781 gtgctcagaa tggcggcgac cgcaccggcg gtcgagatgg ccatcaacca ctggccgcct
      841 atcatctcgg cgaccttgag cacggccatg ttcgtggcca tgcccacttt ccccgcccct
      901 atccatgcga tcagctgtgg gtagaccaga atcagggtga tgaaacccag aaagatactg
      961 cagaaataga agatgcccaa ggcgatgacg cacagggtga cactcttctt ggccgcgctt
     1021 gcactcggta cggtgaagaa catgatcaga atgtgcggca gaccggcggt gccaaagacc
     1081 agagctagta cggtgctgat ctgggcaaag gtgctcggcg tctgcacgcc gatcgtcatg
     1141 gccgtcgccg cgtcgggcat gctggcacgt acagcggtga acgccgcggt cgcgctggcg
     1201 ggaatgaccg tgccgtattt ggccgccaga gccgggtcga tcgccgcacc ggcgctgaca
     1261 gctgccgctg aggcagcaac cgcacccgcg ttggggccgg cgatgcttgg cggcacgatc
     1321 tggcctgccg tctcgaagat cttgttgatg ctgccgtcga agaacatgaa cacgccggcg
     1381 atcacgataa ccaccatcgc ggcccacaga aagatggcct gcacgacctg attgtacaag
     1441 gtcgctttca tgccgccgta gatgatatac agcgagatca gcacgccgat accgatcgtc
     1501 gtgaccagaa agttccagcc cagcagcagt tcgaacacca ggccagcgcc cagcacctgc
     1561 ggcacgaggt agaaggtcga gatgaccaga gtcgtaacca tgacgatcag tttgatgcga
     1621 tcgtctggga aacgtcgata gagcgcgtca gcgacggtgt aactgccgac gttgcgcagg
     1681 ggcccggcga tgagcatgag aagcgccata tacccgccga agaagccgat ggccagccag
     1741 atgcgatcga cacccttgat tgcgatgtca ccggcgacgc cgagaaagct ggcggctgac
     1801 agatagctgc cgaccatcgc gatgccgacc tgcacccacg ggacctgatt gcccgcgacg
     1861 tagaactccg aagtgctctg gcctttgttc aacgtcaggt aggtgatgag aatcgacaga
     1921 aagacgaacc cgagcgtgaa aatgaaaact aagggatatt cacccatgac ttatctccag
     1981 accagaaagt agacgaccag aatcagccag gaaacgggaa acacggcaag agacagaaac
     2041 atgccgagcg gcatgcccat gcacggcatc gcggcgaatt cgcgatatgg cggggtgttg
     2101 ataatcgcca cccacatgta gaacgcgaag aagaccgcga atagcacgaa cccgaccttc
     2161 atgcgacggc gatagttttc ttcggcttcg gtgtcgagag gggaagggtt cacagtttca
     2221 ggcgcagcat caaccataca atcgtctcct tgtgaggacc agaatgtgtt ttcgtcttcc
     2281 cgaaaggctt cgcggtgaga ggcgtctcga aacccgcgac ggcgacctag gcatggccgg
     2341 atcggtcttg tcaggtcgcg gaatgaaaca cgtaaggagg cgaaatttcg ggaagaacgt
     2401 cgaacttctc tatcataatt cctgatggtt cttttcgcaa catggtaaaa tgtgaatgtg
     2461 aaatctttta cattccaagc gattgtttcc gtcgttctcg cgcttctgat cttcttcctg
     2521 gcggtctttc tcaagattct gccggccttc gaacgggcga ttctcgaaca gaaacgcgag
     2581 atgattcgcg aactgaccaa ctcggcctgg aacattctcg cgcgcttcga aaccgacgaa
     2641 cgggccggac gattgacgcg ggacgaggcg cagaaacagg cggtgcagca gattcgcaac
     2701 ctgcattacg gccgcgaaat gaaagactat ttctggatca acgattttg
//
LOCUS       DAORHD010000623         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43524_length_2747_cov_3.040490, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000623 DAORHD010000000
VERSION     DAORHD010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43524_length_2747_cov_3.040490"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1655)
                     /locus_tag="PKO06_13125"
     CDS             complement(216..1655)
                     /locus_tag="PKO06_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNV70637.1"
                     /translation="MKRIFLCMLALLAFATGAWAVESLVQIKGLSHDQIVDMANAGYD
                     IPAHGPDFIEVVVDTQQLKTLKQEGVEHKVVVSDLGAMVRRRLSAQKEAAQPYYTYAT
                     MVETMKKWVAAYPTICRMESIGKSYENRDIWALKISDNPEKNEKEPAALIMGAHHSRE
                     WISVEVPMATAKAMLEGFGTDERLTRLVNERETWFVPMVNPDGVTFSQTSQQFWRKNR
                     RKNANGTYGVDPNRNYGYKWGAVGSSNSPSADTYHGTGPFSEPETCAIRDFATREQFS
                     ADISFHSYSELILYPFSWGYPDEAPNPHQELFATFAQEMAAFNRYRPQVSSDLYPSMG
                     DTDDFMYGELKSLSFTFELARQFIPPQAEIPKINAVNVPAVLHLIEKAGTYGLVSPTG
                     EEQITELSATDLIAAIKDLTPLAKADDIAARLQKVQNQLVLRVLNERRHGESTILTQI
                     EKEAANHPAFSGILRLYGQGVRFDQLHHD"
     gene            1902..2477
                     /locus_tag="PKO06_13130"
     CDS             1902..2477
                     /locus_tag="PKO06_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70638.1"
                     /translation="MKKLMSVLLLVAVACFLPGAVVAEVLSHDAAKVEITVPDKWTQK
                     PEGDVLTITSPDGGMAVVFMVLEQKDADKAFEAVDKELEKSLGDITWANDGKPSEAEI
                     NKMTASEWNGTAKEGKVAVDVLSIDTPSDKNLGIYWFTDVANEKKYEADVETIVKGLK
                     PMGGAAPAAEPAAEPAAEPAAAPAGEGDAGE"
     gene            2568..>2747
                     /locus_tag="PKO06_13135"
     CDS             2568..>2747
                     /locus_tag="PKO06_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70639.1"
                     /translation="MTRTESRWVLLMTMAILGIGIWGVLRLGNRLNPSPASIELLNAV
                     QKRISIHLVQGVAGSR"
BASE COUNT          598 a          679 c          849 g          621 t
ORIGIN      
        1 gcagtttttt gtagtttttg ccggtcagct tcacgtagcc gaaatcaacc gcctcggcgg
       61 ccacaccgcc ggcgaacaac aggacgagaa cgagggccag ggacatgagg tgtcgtcgct
      121 gcataacctt tctcctctga tactatctgt attttcggtc ttggattcag actcaacggc
      181 ttgatcgacc ggcggtcgat caagccgttt ttgcttcagt cgtggtgcag ttggtcgaag
      241 cgcacaccct ggccgtagag tcgcaggata ccggaaaacg ccgggtgatt cgccgcttct
      301 ttttcgatct gggtgagaat ggttgattcg ccgtggcgcc gctcattcaa tacgcgcagt
      361 accagttggt tttgcacttt ctgcagacgg gcagcgatat catcggcctt ggcaagcggg
      421 gtcagatctt tgatggcggc gatcagatcg gtggccgaaa gttcggtgat ctgctcttca
      481 cctgtcggtg acaccagacc gtacgtgccg gccttctcga tgagatgcag cacggcggga
      541 acattcaccg cgttgatctt gggaatctcg gcctgcggcg gaatgaactg gcgggccagt
      601 tcgaaagtga acgacagtga cttcaattca ccatacatga aatcgtcggt gtcgcccatc
      661 gaaggataca ggtcggaact gacctgcgga cggtagcggt tgaaggcggc catttcctga
      721 gcgaaggtcg cgaacagttc ttgatggggg ttcggtgctt cgtcagggta accccagctg
      781 aacggataca ggatcagttc ggaatagctg tggaacgaga tatcggcgga aaactgttcg
      841 cgggtcgcaa agtcgcgaat ggcgcaggtt tcgggttcgc tgaacgggcc ggtgccgtga
      901 taggtgtcgg cgctcggaga attgctcgaa ccgactgcac cccacttgta accgtagttg
      961 cggttcgggt cgacgccgta ggtgccgttg gcattcttgc ggcggttttt gcgccagaac
     1021 tgctgagagg tctggctgaa ggtgacgccg tcgggattga ccatgggtac gaaccacgtt
     1081 tcgcgctcgt tgaccaggcg ggtcaggcgc tcgtcagtgc cgaagccttc gagcatcgcc
     1141 ttggcggtgg ccatggggac ttcgactgag atccattcgc gggagtggtg agcacccatg
     1201 atgagtgctg ccggctcttt ctcgtttttc tcggggttat cgctgatctt gagcgcccag
     1261 atatcgcgat tctcgtaaga tttgccaatg gattccatgc ggcagatagt cgggtaggcc
     1321 gccacccact tcttcatggt ttcgaccatg gtcgcatagg tgtagtaagg ctgcgctgct
     1381 tctttctgag ctgacaaccg tcgacgcacc atggcaccga ggtcagagac gacgactttg
     1441 tgctctaccc cttcctgttt gagggttttg agctgctggg tgtcgaccac gacttcaatg
     1501 aaatcaggac catgagccgg aatgtcatac cccgcgttgg ccatgtcgac gatctgatcg
     1561 tgcgaaaggc ccttgatctg aaccaggctc tcgacagccc aggcaccggt ggcaaaagcc
     1621 agcaaagcaa gcatgcagag aaagatacgt ttcatgattc agttgctcca tacaccagac
     1681 ttcgaatcag cgaaccaggc gaacatacat cgtactccgg gatagtttac ctccagagat
     1741 atgagtttgg caaatgaatc cttacgatgg acaatgagca gattggacag ggggaaaacg
     1801 aaagttgtac aaattaagtt gctacatctc cggatgagtg atactgattt tgtgtctcat
     1861 ttattcgtga gacgtaattc tggaataggg gagtacgatt tgtgaagaag ctgatgtcag
     1921 tgttgttgct ggttgcggtt gcttgtttcc tgcccggcgc ggtcgtggct gaagtgttgt
     1981 cgcacgatgc cgcaaaggtt gagatcacgg ttcccgataa gtggacccag aagccagaag
     2041 gcgatgtcct gaccattacc tcccccgatg gcggaatggc ggtggtgttc atggtcctgg
     2101 aacagaaaga cgcggacaag gcgttcgagg ctgttgacaa ggaactcgag aagtcactcg
     2161 gtgacatcac ctgggcgaat gacggcaagc cgtctgaagc cgagatcaac aaaatgaccg
     2221 cttcagagtg gaacggcacg gccaaggaag ggaaggtcgc ggttgatgtt ctgtcgatcg
     2281 acactccttc cgacaagaac ctgggtatct actggttcac cgatgtggcc aatgagaaga
     2341 aatatgaagc ggatgtcgaa accatcgtca agggtctgaa gccgatgggc ggcgcggctc
     2401 cggcggcaga accggcagca gaaccggcag cagaaccggc agcggctccg gctggtgaag
     2461 gcgacgctgg cgagtaagat ctctttcgct caaaagcacg aagcccgggg cgagtcgccc
     2521 cgggcttttt cgagttcgct gatacggagg agaagaagat gcagggtatg accagaacgg
     2581 agtcacgatg ggtgttgttg atgacgatgg cgattctggg aatcggtatc tggggagttt
     2641 tgcgcctggg gaatcgatta aacccgagcc ctgcctcgat cgaactgctg aacgccgttc
     2701 agaaaaggat ttccattcat ctggtgcaag gggtcgccgg ttcgaga
//
LOCUS       DAORHD010000624         2746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43537_length_2746_cov_3.382386, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000624 DAORHD010000000
VERSION     DAORHD010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43537_length_2746_cov_3.382386"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKO06_13140"
     CDS             <1..430
                     /locus_tag="PKO06_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70640.1"
                     /translation="VDYTLRDRHREAYTFYRDLIAFRHSQAGSLLKRVTADMCRNVQV
                     VLCNNHNAVGLFWHERPEEFSRAEPGRQLLVLFNPNAQQSVLFQPRLPPGSWFRIVAG
                     GKVVSRNSVFSRTLEVSTGDIGGMPIEVPPLDVQVWIPAE"
     gene            470..>2746
                     /locus_tag="PKO06_13145"
     CDS             470..>2746
                     /locus_tag="PKO06_13145"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV70641.1"
                     /translation="MDLTVLRFALTSFLGSFLSFVMQPMAGKAVLPVHGGSYMVWAAT
                     LCFFQLTLLLGYAFAHLLFSHRVTVHRCLFWTITLIVTAIAVPPVLDPLRRSAPLFAS
                     PGIDILGRLVIGFGSPAVMLFAGSIMLQRFWRSTPGSAGREPLIYYAASNVGSLGALI
                     GYLFFIEPHLSLVTQEYALRLLYPFWILCFVTCIPWQPTEELDPSLFLSTSNASPSSA
                     SGVDREQPVLAPAVVSSMVDRGALEPAIAAPDTIPELPAHALFLQAAAGSSLLASTTT
                     LLTLDVSPMPFLWVAPLTIYLLTYIRVFSHSESAHHAATRWFVPALAIGFSLALIHQL
                     NFALGILPRLALHLAVLYYLCLACHSRFLVAGPPAPHRLTRFYLFIAAGGAAGTVAVN
                     LLAVFAFDRLQEFNLCLLLTAAAFRGVTLPPTTLTRRATEADCSWSFAGDPFFVLRAL
                     AIIVLFLVFPSLVNQGMPITSSFLPPVAAGAMVYGWLALRTAERLGGRETASALLIAV
                     SILFLPDIGAPGTEHLSRRTWYGLYRVYDQGDIRFLQMGSTFHGGRWLDPTRAHEPLL
                     YYHPETTIGRFFSTQSPTWQRAAAIGLGAGMLSAFTRPGQELTYLELDPVQVDIARRW
                     FPFLDHASGSITVQTGDGRLLLRNATSTFDLIVFDAFSSDSIPTHLLTLEAHREALLK
                     LASGGMLLYNISNRHLNLLPVLAANADVLGLSMLVSRNQDSDDPWRLPTRWVALGTRS
                     ALARHLGAHDQWLPLPRKP"
BASE COUNT          415 a          951 c          723 g          657 t
ORIGIN      
        1 cgtcgattac acgctccgcg accgtcaccg tgaggcgtat accttctacc gtgatctgat
       61 cgcgttccgt cattcccagg ccgggtccct gctgaaacgc gtcaccgccg atatgtgccg
      121 caacgtacag gtcgttcttt gtaacaatca caatgcggtg gggttgttct ggcacgaacg
      181 tcccgaagag ttttcccggg ccgaaccagg ccgtcaactg ctggtactct tcaatccgaa
      241 tgcacagcag tccgtgctgt tccaaccgcg tcttccccct ggcagctggt ttcgcatcgt
      301 cgccggcggc aaagtggtca gccgcaacag cgtgttttcg cgcactctgg aagtcagcac
      361 cggcgacatc ggcggcatgc ccattgaagt cccgccactg gacgtgcagg tctggatccc
      421 ggcggaatga tccggagagt atcaccccgg actcccacgt gacgccgtca tggatttgac
      481 cgtcctgcgt tttgctctga cctcctttct cggttcgttt ctctcgtttg tcatgcaacc
      541 gatggcggga aaagcggtgc ttccggttca tggcggcagt tacatggtct gggcggcgac
      601 cctgtgcttc tttcaactga cgctgctgct tgggtatgcc ttcgctcacc ttctgttctc
      661 tcatcgcgtg accgtgcatc gttgcctctt ctggaccatc actctgatcg tcacggccat
      721 cgccgtgcca cccgtgctcg atcccctgcg acgctccgcg cccttgttcg cgagtcccgg
      781 gatcgatatc ctgggacgac tggtcatcgg gtttggcagc ccggccgtca tgctgttcgc
      841 gggcagtatc atgctgcaac gtttctggcg aagcaccccc ggctctgccg gtcgcgaacc
      901 actcatctac tatgcggcat ccaatgtcgg ttcgctcgga gcgctgatcg gctatctgtt
      961 cttcatcgag ccgcacttgt ccctggtcac gcaggaatac gcgcttcgcc tgctgtatcc
     1021 gttctggatc ctctgcttcg tcacttgtat tccctggcag ccaaccgaag aactcgatcc
     1081 atctttgttt ctcagtacga gtaatgcatc gccttcttca gcttctggtg ttgacagaga
     1141 gcaaccggtt cttgcacccg cagtcgtttc gtcgatggtc gatcgaggtg cgctcgaacc
     1201 agcgatcgca gcgcccgaca ccataccgga actgccggct cacgccttgt ttctgcaagc
     1261 ggcggccggt tcatcgcttc tcgcctctac cacgactctt ctcaccctgg acgtttcgcc
     1321 gatgccgttc ctctgggtgg cccccctgac catctatctg ctgacctaca ttcgcgtctt
     1381 cagccacagt gagtcggctc accatgcggc gacgcgatgg ttcgttcccg cactcgccat
     1441 cgggttttcc ctggccctca tccaccagtt gaacttcgct ctcgggattc tgccgcgatt
     1501 ggccctgcat ctcgcggtgc tctactatct ctgtctggcc tgccactccc gctttcttgt
     1561 cgccggccca ccggcaccgc accggttgac ccggttctat ctcttcatcg cggcgggcgg
     1621 cgcagccgga accgtggccg tgaatctgct cgccgtgttc gccttcgacc gcctgcagga
     1681 attcaacctg tgtctgctgc taacggccgc ggcattccgc ggtgtgactc tgcccccgac
     1741 aacactgacc cgccgcgcca ccgaggctga ctgttcgtgg tcattcgccg gcgacccctt
     1801 ctttgtgttg cgcgcgctgg cgatcatcgt tctctttctt gtgtttccaa gcctcgtgaa
     1861 tcaaggcatg ccgatcacca gttcattcct gccaccggtc gccgccggcg ccatggtcta
     1921 tggctggctc gccctgcgca ccgccgagcg gctcggcggg cgtgaaaccg cttccgctct
     1981 attgatcgcc gtctcgatcc tgttccttcc cgacatcggc gcgccgggta cggagcatct
     2041 ttcccggcgc acctggtatg gtctctatcg cgtctacgac cagggcgaca ttcgttttct
     2101 ccagatggga agcacgtttc acggaggtcg ctggctcgat cccacccgcg cgcacgaacc
     2161 tctgctctac taccatcccg agacgaccat cggccgcttc ttctcgaccc agagccccac
     2221 ctggcagcgc gccgcggcga tcggtctcgg cgccggcatg ctgtcggctt tcacccgccc
     2281 cggccaggaa ctgacctacc ttgaactcga tccagtgcag gtcgacatcg ctcgccgctg
     2341 gtttcccttt ctcgaccacg cctccggaag catcaccgtt cagaccggcg atggacgcct
     2401 tcttttgcga aatgccacca gtactttcga tctcatcgtc ttcgatgcgt tcagcagcga
     2461 ctccattcct actcatctgc tcacgttgga agcccatcga gaagcgctgc tcaagctggc
     2521 ttcgggtggt atgctgctct acaacatctc aaaccgccac ttgaatcttc tgcccgttct
     2581 tgcagcgaat gccgacgttc tcggtctttc aatgctggtt tcacgcaacc aggactcgga
     2641 cgatccgtgg cgattgccca cccgctgggt agccctcggc acgcgttcgg cgttggcgcg
     2701 acacctcgga gcacacgatc agtggctgcc gctgccgcgc aaacca
//
LOCUS       DAORHD010000625         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43561_length_2745_cov_3.484015, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000625 DAORHD010000000
VERSION     DAORHD010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43561_length_2745_cov_3.484015"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="PKO06_13150"
     CDS             complement(<1..1499)
                     /locus_tag="PKO06_13150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70642.1"
                     /translation="MKRLRLAILGLLLVTGPISALSLDTLPDYQDAGMKLYVRGTQLL
                     QENDFVSAADELRRAVKARPDMAEAFHNLGYALEKTGDLKNAAAAYERAISLKPTYAS
                     AFNNLGFLLATAEVDKARAVQLCRRAVELEPNSAAYRDSLGWASYKAGRLEDAVMHFQ
                     AALKLDANYAKSWFNLGLCEFSRKNYAEAARNFTGAIQLNTNLLKAYLPLAESFERMQ
                     QTNRALNVYQQALSKAPETSPVRKHIERQIKRLTAGSKSYYFSNVKQLQGSAKLTDFM
                     RRRGKTGNLAGTTSKVVDPLDSSSTFTPVSLAQAPTAGVSGTDLSDYALGGEPTYAAP
                     VVPMARSMNAAPATTPRFDSELSVNAERSLEKKYSLCQSYIERGLVQEAAKELENIVT
                     VAGTSSIGRQARNLLLRARKMIDERNAERAGTHLTMGKDFIRSGKYDMAETELKKSLS
                     LTPENAEAHKDLALLYYNQNRFREAYESSKRAIALDRTMKEAYVVLASL"
     gene            1728..>2745
                     /locus_tag="PKO06_13155"
     CDS             1728..>2745
                     /locus_tag="PKO06_13155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV70643.1"
                     /translation="MPGLLLVSLVLRLYRIGEESLWLDEIETVRLVQLPFGEMMNYIV
                     HADSHPPLYSLLLHLWVRIAGTSETAVRFWSAIFGCLSVWATYLTGTVIFNRRTGLIA
                     GLLLAIAPFHVYYSQEARMYALLGLFAAGMMLALWQVTFDQRSRWRYLLVVTSVGALF
                     TQNIALFLIVLQNLWYLLTRHCHHMRPRQWLYLQITIVSLAFFWLPGLYLQLHATRNI
                     GFLSWIQSPTLAHVADVLFEFCGAFPIHSESEPSIPYLGAAGILSLGFLVAFLVPFIT
                     QIRHSLNSGSSTTSDPDFSATVFDNGTSVMSVPVATRLRCHQILYLGLWFVIPIVSPL
                     LLSYV"
BASE COUNT          507 a          830 c          734 g          674 t
ORIGIN      
        1 tacagagagg caagcacgac atacgcttcc ttcatggtac gatcaagggc aatggcacgt
       61 ttgctcgatt cgtaggcttc gcgaaaccgg ttctggttgt agtaaagcag agccagatcc
      121 ttgtgagcct cagcgttctc gggagtgagt gacaacgatt tcttcaactc ggtttcggcc
      181 atatcgtatt ttcccgagcg aatgaaatcc ttgcccatgg tcaggtgcgt gcccgctcgc
      241 tcagcgttcc gttcatcaat catttttcgc gcccgcagga gcagattccg cgcctgccgt
      301 ccaatcgacg aggtgccggc gacggtgacg atgttttcga gctcttttgc tgcctcttgt
      361 accagtccgc gttcgatgta agactgacag agactgtatt tcttttcgag cgagcgttcg
      421 gcattcaccg acagttcact gtcgaaacga ggggtggttg ccggcgcggc gttcatcgag
      481 cgagccatcg gcacaacagg tgcggcatac gtcggctctc cacccagcgc ataatccgag
      541 aggtccgttc cggacacgcc cgcagtggga gcctgggcga gcgacaccgg cgtaaacgtc
      601 gacgagctgt cgagtgggtc aaccaccttg gaagtcgtgc ctgccagatt gcccgtcttg
      661 ccacggcggc gcatgaagtc cgtcagtttg gcactgccct gaagttgctt gacgttcgag
      721 aagtagtacg atttgcttcc ggctgtcagc cgcttgatct gccgctcaat gtgtttgcga
      781 accggcgagg tttcgggtgc cttcgataga gcctgctgat aaacattcag cgcgcgattg
      841 gtctgctgca tgcgttcgaa cgattcagcc agaggcagat acgccttcaa cagattcgta
      901 ttcaactgaa tcgctccggt aaagttgcga gcggcttcgg cgtagttctt ccgcgagaac
      961 tcacagagac cgagattgaa ccaggatttc gcgtagttgg cgtcgagttt cagcgctgcc
     1021 tgaaaatgca tcaccgcgtc ttcgaggcga ccagctttgt aactggccca cccgagagaa
     1081 tcgcgatagg cggccgagtt tggttcgagc tcaacggccc gccgacacaa ctgcacggct
     1141 cgcgccttat ccacttccgc ggtggccagc agaaagccca gattgttgaa ggccgacgca
     1201 tacgtcggct tcaaggaaat cgcccgctca taggccgcgg ctgcattttt caagtccccc
     1261 gtcttttcca gcgcatagcc caggttgtgg aaagcctcgg ccatgtcggg acgcgctttg
     1321 acggcgcgcc gcagttcgtc ggccgccgag acgaagtcgt tctcctgaag cagctgcgtg
     1381 cctcgtacgt aaagtttcat cccggcatcc tggtagtccg ggagtgtgtc gagcgagagc
     1441 gccgagatcg ggccggttac cagtaacagg ccgagaatgg ccagtctgag tcgtttcatc
     1501 ggttgcctcc tgatggcaag tcgcgaaagc agcacgcctt ccgcgagaaa cactttttct
     1561 caaatcctca tcatcgacag aacggctcct ttcccgaagc cctgacggtt gaaggttgcg
     1621 aaagagtcgg aaacatcgca tcatgcatct tgctcatgac gtgcgacacg ccgcgtgttt
     1681 tcaacatgac tcattcaacc ttcctcaatt cgtgcagcag actgtgcttg ccgggccttc
     1741 tcctggtcag cctcgttctt cgtctctacc gtattggcga ggaaagtctc tggctcgacg
     1801 agatcgaaac cgttcggctc gtacagctgc cattcggaga gatgatgaac tacatcgttc
     1861 atgcggactc acatcctccg ctctactcgc tgttgctgca cctctgggtg cgaatcgccg
     1921 gcacatcgga gacagccgta cgcttctggt cggcgatctt tggctgttta tccgtgtggg
     1981 ccacgtatct gaccggtacg gtcatcttca atcgcagaac gggattgatc gccggcctgc
     2041 tgctcgccat cgcccctttt cacgtctatt attcgcagga agcgcgcatg tatgcgcttc
     2101 ttgggctgtt cgccgccggc atgatgctcg cgctgtggca ggtgactttc gaccagcgct
     2161 cgcgatggcg ctacctgctc gttgtcacca gcgtcggggc cttgttcacc cagaatatcg
     2221 ccctctttct gatcgtgctg cagaatctct ggtatctgct cacccgtcac tgccaccaca
     2281 tgcgcccgcg tcagtggctc tatcttcaga tcacgatcgt ctcgctcgcg tttttctggc
     2341 tgcccggact gtatctccag ctccacgcga ccaggaacat cggttttttg agctggattc
     2401 aatcaccgac tctggcccat gtcgccgacg tgctcttcga attctgcggc gcgtttccga
     2461 ttcactccga gtccgagcca tccattccct atctgggcgc agccggaatt ttgagcctcg
     2521 gttttctcgt cgcttttctc gttccgttca tcacccagat tcgtcactct ctcaacagcg
     2581 gcagttccac gaccagcgac ccagactttt cggcaaccgt attcgacaac ggcacctcag
     2641 tcatgagtgt cccagtcgca acacgtcttc gctgccatca aatcctctat ctcggcttat
     2701 ggtttgtgat tccgattgtc tcccctcttc tcctctctta cgttt
//
LOCUS       DAORHD010000626         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43598_length_2743_cov_4.723214, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000626 DAORHD010000000
VERSION     DAORHD010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43598_length_2743_cov_4.723214"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PKO06_13160"
     CDS             complement(<1..1263)
                     /locus_tag="PKO06_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HNV70644.1"
                     /translation="MLSFTSPWILTGLLTIGIPVYLHLYYRRTPVPRDFPSLRLIRQS
                     VEQLYNRMQIRNWILLVLRCLVILFLVSAFARPFWGAGSGGPVQKGAPTAFVIALDTS
                     MSMGASHQGISLFNAAKAKAIEILDRMGPYDKGAIAAIHDPGLLLSPQLTWDKNELKE
                     TVRNLPLSWAGTNLGSSLQPALKLLSQVRNTKRTLYVITDLTTQAWKPFTDTYDLKRI
                     DKDIDLVLIPIGNEAPVNLSVSKLTCEAPVVIKGRSAPVAITVSNHSARSQKSVLSLY
                     LDGEKKWETPVDLDARGSKRLLASCTFPQAGVSQIRATLTGDTLPQDDQRHLAVKVLT
                     APRILILHPPVDREGRETRDDLYLRFALAPLGRQEGATFLVERRLPEETNSLALEAFS
                     AIFLVNQRQLSDALLKRLQDYLMGGGNLV"
     gene            complement(1266..2183)
                     /locus_tag="PKO06_13165"
     CDS             complement(1266..2183)
                     /locus_tag="PKO06_13165"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV70645.1"
                     /translation="MESQRRLSRYLDPKLLASLANLEIKARGVVEGFIEGLHKSPFKG
                     FNVEFAEYRQYVPGDPIKDIDWKVYARTDKYYIKEHEEETNLSGYLIMDTSGSMSYKG
                     DGLLNKMDYASTLAASLAYLMLRQQDSVGLVTFAEGVRKIIRPRGGMAHLKAICTEFE
                     QTKPTDKTDIGESLDRVAETMRKRGLLIIFSDLLDNAATVINKARLLRSRRHEVLIFH
                     ILDRHELNFPFEDTTLFRDLEDRSELVIDAYSLRREYLRRIQAFVGTFKSALRRGGID
                     YLLTDTSRPLEASLKSFFTRRQSMGHGVA"
     gene            complement(2195..>2743)
                     /locus_tag="PKO06_13170"
     CDS             complement(2195..>2743)
                     /locus_tag="PKO06_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV70646.1"
                     /translation="PFFVLATQNPIEQEGTYPLPEAQLDRFMFNIRIEYPPHEEEVAM
                     VKATTTGSEPTLSPVITRERILQLQDMVRRVPVSDHVVRYAVAIAEATRPNQPKTQQY
                     VNDFVTWGAGPRASQYLILGGKSRALLKGRFYVACEDIRAMALAVLRHRIILNFNAEA
                     EGITPDTVIQRVIQSIPEPSSF"
BASE COUNT          521 a          746 c          909 g          567 t
ORIGIN      
        1 caccagattg ccgccgccca tgaggtagtc ctgcagacgc ttgagcagag cgtcggacag
       61 ttgacgttga ttgaccagaa agatggccga aaacgcttcg agtgccaggg agttcgtctc
      121 ttcgggcaag cgtcgctcga ccagaaaggt ggctccttcc tggcggccca gcggggcgag
      181 cgcaaagcgc agatacaggt cgtcacgggt ctcgcggcct tcgcgatcga ccggcgggtg
      241 aaggatcaga atgcgtggcg cggtcaggac cttgacggcg aggtgcctct gatcatcctg
      301 cggcagcgta tcgccggtca gggtggcgcg gatctgactg acaccggcct gcggaaacgt
      361 gcaggaggcc aacaggcgct tcgatccgcg agcgtcgagg tcgaccgggg tttcccactt
      421 cttctcgccg tcgagataca acgacagaac cgatttctgg gaccgggcgc tgtgattgct
      481 cacggtgatg gcgaccggag ccgagcgccc cttgatcacg accggggcct cgcaggtcag
      541 tttcgaaacg gaaaggttga ccggggcttc gttgccgatc ggaatcagta ccaggtcgat
      601 gtctttgtcg atgcgcttca gatcgtaggt gtcggtgaac ggtttccagg cctgggtggt
      661 gaggtcggtg atcacgtaga gtgtgcgctt ggtgttccgt acctgtgaca agagcttgag
      721 agcaggttgt agagatgatc ccaggttggt gccggcccaa ctcaggggca ggtttcgcac
      781 tgtttccttg agttcgtttt tgtcccacgt gagttgtggc gacagcagaa ggcccgggtc
      841 gtgaatcgcc gcgattgcgc ctttgtcata ggggcccatg cgatcgagaa tctcgatggc
      901 cttggccttc gccgcgttga acagagagat gccctggtgc gacgcgccca tcgacatcga
      961 agtgtcgaga gcgatcacga acgcggttgg agcacccttc tgcaccggac cgccgctgcc
     1021 cgcgccccag aacggtcgcg cgaatgcact gaccagaaag agaattacga ggcagcgcaa
     1081 taccagcaga atccagtttc tgatctgcat gcggttgtac aattgctcga cggactggcg
     1141 aatgagtctc agactgggaa agtcgcgcgg cacgggcgtg cggcgatagt acaagtgcag
     1201 gtaaacggga atgccgatgg tcagcagtcc ggtgagaatc caggggctgg tgaagctgag
     1261 catgctcagg cgactccgtg gcccattgat tggcgccggg tgaagaagga cttcaggctt
     1321 gcctcgagcg ggcgcgaggt gtcggtcagc aggtaatcga taccgccgcg ccgcagcgcg
     1381 cttttgaagg tgccgacgaa tgcctggatg cgccgcaggt attcgcgtcg cagactgtag
     1441 gcatcgatga ccagttccga gcgatcttca agatcgcgga acagggtggt gtcctcgaag
     1501 ggaaagttca gttcgtgccg gtcgaggatg tgaaagatca gcacctcgtg tcgccgcgag
     1561 cgtaacagac gggctttgtt gatgaccgtg gccgcgttgt cgagcagatc agaaaagatg
     1621 atcagcagac cgcgcttgcg catggtttcg gcgacccggt cgagtgattc cccgatgtcg
     1681 gtcttgtcgg ttggcttcgt ctgctcgaat tcggtgcaga tggccttcag atgcgccatg
     1741 ccgcctcggg ggcgaatgat cttgcgcaca ccttcggcga aggtgacgag cccgacgctg
     1801 tcctgctggc gcagcatcag ataggcgagc gaagccgcga gagtcgaggc gtaatccatt
     1861 ttgttcagca gcccgtcgcc cttgtagctc atcgagccgc tggtgtccat gatcagatac
     1921 cccgaaagat tcgtctcttc ttcgtgttcc ttgatgtagt atttgtcggt gcgcgcatag
     1981 accttccagt ctatgtcttt gatcggatca cctgggacat attgtcggta ctcggcgaac
     2041 tcgacattga accccttgaa cggactcttg tgcagaccct cgatgaagcc ctcgacgact
     2101 ccgcgggcct tgatttcgag gttggccagc gaagcgagaa gcttcgggtc gaggtagcgc
     2161 gagagccgac gttgcgattc catgtggggc tgacttagaa actgctgggt tcgggaatcg
     2221 actgaatcac gcgctgaatc acggtgtcgg gagtgatccc ttccgcttcg gcattgaagt
     2281 tgagaatgat gcggtggcgc agcaccgcca acgccatggc gcgaatgtcc tcgcaggcca
     2341 cgtagaagcg gcccttgagc agcgcgcggc tcttgccgcc gagaatcaga tactggctgg
     2401 cgcgcggccc ggctccccag gtcacgaagt cgttaacata ctgctgggtc ttcggctggt
     2461 tcggccgggt cgcctcggcg atggcgaccg catagcgcac gacgtgatcg gaaacgggga
     2521 cacggcgcac catatcctgc agctggagaa tgcgttcgcg ggtgatgacc ggcgacagcg
     2581 ttggctcgct gcccgtcgtg gtggctttga ccatcgcgac ttcttcttcg tgcggtgggt
     2641 actcgatacg aatgttgaac atgaaacgat cgagctgggc ttcaggcagc gggtaggtgc
     2701 cttcctgttc gatcgggttc tgggtggcca gcacgaagaa cgg
//
LOCUS       DAORHD010000627         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43785_length_2735_cov_4.440299, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000627 DAORHD010000000
VERSION     DAORHD010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43785_length_2735_cov_4.440299"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PKO06_13175"
     CDS             complement(<1..638)
                     /locus_tag="PKO06_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV70647.1"
                     /translation="MSPGRPRVLLVYTTPIKPDAFFGFVLPPLGLERLAACLEPIADV
                     RILDLRFEGNAWLESALAFQPDVIGVNVKTTMYATHSYRMADRLRARLPRTRLILGGL
                     HASSVPEEASEHGDVVVIGDGEQAVVEIVQGRPLREINGICYREAGKPIRTAPRPLLE
                     NLDELPPPARHLRPAHYRYMAAKLIPMDLLETSRGCTQYCPFCSPAITHKGK"
     gene            complement(638..1387)
                     /locus_tag="PKO06_13180"
     CDS             complement(638..1387)
                     /locus_tag="PKO06_13180"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNV70648.1"
                     /translation="MDLRLDGKRVIVTGGTRGIGRAIATTFAQAGAKLVINYHRNGKA
                     ADETVEELRQLGCEPLLVKGNAADVEFGRHLVERAVQAMGGVDILVNNAAVSHNTPFL
                     MLETDEWHEAVDVNINALFSVTQPVLRQMIEQRIHGSILNLTSVCGVRPVAAVPVHYA
                     TTKAAIHGFTYTLAKEIGRYGIRVNGIAPGLIETAFAANLPEARHEDFRKFCPMQRIG
                     KPAEVANLALFMVSEFNSYMTGETVVVSGGL"
     gene            complement(1390..1782)
                     /locus_tag="PKO06_13185"
     CDS             complement(1390..1782)
                     /locus_tag="PKO06_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNV70649.1"
                     /translation="MDQSIFDGVQEAIQDSLGIPREKITLKARIIDDLGSDSLDLLDI
                     LFAIEERFGIKIKRGQIEAMARDGMSEGEFERDGLLTAQGAERLKQIMPEVDQAQIVE
                     GMGVYKIPYLFTVETFCRLVERKMAEKR"
     gene            complement(1688..>2735)
                     /locus_tag="PKO06_13190"
     CDS             complement(1688..>2735)
                     /locus_tag="PKO06_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70650.1"
                     /translation="GLFTRLLAPIQETEETDTVVRFPLWFSSNGTIETERAVDIEIDV
                     DEDSYAGSMPRHMIGKEGYRFGVTGRPVVLVTNALHLALANMAANVARLAELKKMSLW
                     QKIVTLAKARSINKFKVLSAVSQIHPEAEVHPTAVVEGSVIEKGAKIGAYAVVRFSYV
                     GEGAFIDDHAGLKFSVIGREAFIANNCVIFFSVVYPRAFLISGPFQFSLFGYDSALMN
                     AIPADYRLDGKTISVRTGPDRSRDTGLRFVGSVIGHRTKIAAGVIIAPGRMIPNDLLI
                     FPDPARVIASVPVSAPTNTPLFLVDGKLVPAEELPRKENTSHGSEHFRRRAGSHSGLT
                     RHTPRKNHPQGSHH"
BASE COUNT          539 a          836 c          794 g          566 t
ORIGIN      
        1 cacttgccct tgtgcgtgat cgccggggag cagaacgggc agtactgcgt acaaccacgg
       61 ctggtctcga gcaggtccat gggaatgagc ttcgcagcca tataccggta atgcgccggt
      121 ctcaggtgtc gagcgggcgg cggcagttca tcgaggttct cgagtaacgg ccgcggtgcc
      181 gttcgtatcg gttttcctgc ctcgcggtag cagattccgt tgatttcacg cagcggtcgg
      241 ccctggacga tctcaacgac cgcctgttcg ccatcaccga tcaccaccac gtctccgtgt
      301 tcagatgctt cttccgggac ggacgaggca tgcagacccc cgagaatcaa ccgggtccgc
      361 ggcagacgtg cccgcagccg gtcggccatg cggtaactgt gcgtggcgta catggtcgtt
      421 ttgacgttga cgccgatcac atcaggctga aaagccaacg cgctctcgag ccacgcgttt
      481 ccttcaaagc gcagatcgag aatccggacg tcagcaatcg gttcgaggca ggcggcaagg
      541 cgctccagac ccaacggggg caggacgaac ccaaaaaagg cgtcgggttt gatcggggtc
      601 gtatagacca gcaatactcg tggtcgtccg ggtgacatca gagccctccg ctgacgacga
      661 cagtctctcc ggtcatgtaa ctattgaact cggacaccat gaagagcgcc agattcgcca
      721 cctcggcggg tttgccgatg cgctgcatcg gacagaactt cctgaaatcc tcatgtcgcg
      781 cttcgggcag gttggcggca aaggccgttt cgataagacc gggagcgatg ccgttcacgc
      841 gaatgccgta gcggccgatc tctttggcca gggtgtaggt aaacccgtga atggccgcct
      901 tggtcgtggc atagtgcacc ggtaccgcgg ccaccggacg cacaccacac accgacgtca
      961 gattcaaaat gctgccgtga atgcgctgtt cgatcatctg ccgcagcacc ggctgcgtga
     1021 ccgagaacag agcgttgatg ttcacatcga cggcttcgtg ccactcgtcc gtttcgagca
     1081 tgaggaaggg ggtgttgtga ctgaccgccg cgttgttgac cagaatatct accccgccca
     1141 tggcttgaac ggcacgctcg accaggtgtc gaccaaactc gacatcggcg gcgtttccct
     1201 tgaccagaag cggttcacac cccagctggc gcagttcttc aacggtctcg tcggcggcct
     1261 tgccgttgcg atggtagttg atcaccagct tcgcgccggc ttgagcgaac gtcgtggcga
     1321 tcgcccgacc gatcccgcgg gtgccgccgg tgacaatgac gcgcttgccg tcgagtcgca
     1381 ggtccatggt caacgcttct ccgccatctt ccgttccacc aggcggcaga acgtttcgac
     1441 cgtgaacaga tacggaatct tgtacacgcc cattccttcg acaatctgcg cctgatcgac
     1501 ttcaggcatg atctgtttca ggcgttcagc tccctgcgcc gtgagcagcc catcgcgctc
     1561 gaactcgccc tcgctcatgc cgtcgcgtgc catggcctcg atctgcccgc gcttgatctt
     1621 gatgccaaac cgttcttcaa tcgcgaacag aatgtcgagc agatccagcg agtcactgcc
     1681 cagatcgtca atgatgcgag ccttgagggt gattttttcg cggggtatgc cgagtgagtc
     1741 ctgaatggct tcctgcacgc cgtcgaaaat gctctgatcc atgcgatgta ttctccttcc
     1801 tgggcagttc ttctgcgggt accagcttgc cgtcgaccag aaacagcgga gtattggtcg
     1861 gcgctgacac cggcacggag gcgatcacgc gcgccgggtc gggaaagatc aaaaggtcgt
     1921 tgggaatcat gcgccccgga gcaatgatca ctccggcggc aatcttcgtt cggtggccga
     1981 tgaccgatcc gacaaaacgc agacccgtgt cacggctccg gtcggggccg gtgcgcaccg
     2041 aaatcgtttt tccgtccagt cgataatcgg ccggaatcgc gttcatcagc gcggaatcat
     2101 acccgaacaa cgaaaactgg aatggaccgc tgatgaggaa cgcgcgggga tagacgaccg
     2161 agaaaaagat gacgcagttg ttggcgataa acgcttcgcg accgatgacg ctgaatttca
     2221 acccagcgtg atcgtcgatg aacgctcctt ctccgacgta ggaaaatcgg acaacggcat
     2281 aggcaccaat cttggcccct ttctcgatca cgctgccttc gaccaccgcg gtcgggtgga
     2341 cttcggcttc gggatgaatc tgagacaccg cgctcaacac tttgaacttg ttgattgatc
     2401 gagctttcgc cagggtaacg atcttctgcc acaggctcat cttcttcagc tcggcaagcc
     2461 tggccacatt cgccgccata ttcgccagcg ccaggtgcag tgcgttcgtc accagcacca
     2521 ccggacgccc ggtgactccg aaccgatacc cttctttgcc gatcatgtgt cggggcatgc
     2581 ttccggcgta actgtcttcg tccacatcga tttcgatgtc gaccgcccgt tcggtttcga
     2641 tggtcccgtt ggagctgaac cagagcggaa atcgcaccac ggtgtcggtc tcttcggttt
     2701 cctgaatcgg ggccagcaga cgggtgaaca gacct
//
LOCUS       DAORHD010000628         2733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43851_length_2733_cov_2.845407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000628 DAORHD010000000
VERSION     DAORHD010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43851_length_2733_cov_2.845407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PKO06_13195"
     CDS             <1..441
                     /locus_tag="PKO06_13195"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNV70651.1"
                     /translation="ELTTREADNLLNKHSGVLGISGESSDLRDLEEDWPKKSEKANLA
                     LEVYAYRIRKYIGAYMAALNGCDALIFTAGVGENSPIIRGIALKNLDFLGIKIDYDVN
                     ERTWRGAEGDIATSDSKVRVLVIPTNEELVIAEDTLDIVKPKKK"
     gene            complement(505..1458)
                     /locus_tag="PKO06_13200"
     CDS             complement(505..1458)
                     /locus_tag="PKO06_13200"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNV70652.1"
                     /translation="MNRKAELKKATRTTAVVAWPNDLKIGFHVSVAGGPKALFERYRA
                     RACQSLQIFVGSPRVWKRTPLSPEYLLEFQTQREAVGNPPLVVHTGYLINLASARPEV
                     RDGSIHLLREEYQLAKALGADYVVQHMGSHAEHKPGLKLMIDGLNEALGGRALEKPLL
                     LLENTAGERNDLGARLEDIARVRAAVDLPTGVCLDTCHAFQAGYDLVSVDGRQDFFDA
                     WRKHLGVEAVRVVHLNDSLNPFAKGHDRHQHIGEGHIGDTAIAALLQHRLLKGMTVIL
                     ETPQDPSGDPRLEWKNLRRLAANLGLPDLRIEPSSRKDSAR"
     gene            complement(1568..1640)
                     /locus_tag="PKO06_13205"
     tRNA            complement(1568..1640)
                     /locus_tag="PKO06_13205"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1604..1606),aa:Glu,seq:ttc)
     gene            complement(1662..1733)
                     /locus_tag="PKO06_13210"
     tRNA            complement(1662..1733)
                     /locus_tag="PKO06_13210"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1699..1701),aa:Ile,seq:cat)
     gene            complement(1776..>2733)
                     /gene="rpoD"
                     /locus_tag="PKO06_13215"
     CDS             complement(1776..>2733)
                     /gene="rpoD"
                     /locus_tag="PKO06_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNV70653.1"
                     /translation="IITELMESNVELVDDDEIRLKEKLRMPEVKKDEAHVDVEDFDDI
                     DDSVRVYLREIGKIRLLTTQEEVELAKRIEHGDQEAQRKLIEANLRLVVSVAKKYTKR
                     GLLFLDLIQEGNQGLIRAVEKFKYRKGYKFSTYAIWWIRQAITRAIADQARTIRIPVH
                     MVETINKIRKASRKLVQKMGRDPLLEELAKEVGLPIEKVKNIMKTAMDPISLETPING
                     EEDSHLGDFVEDKSALPPAETAFNNILKEQINRVLSTLTEREAKVIKFRFGLEDGWQR
                     TLEEVGQKFGVTRERIRQIEAKALRKLRHPSRSKKLKEFYME"
BASE COUNT          553 a          793 c          760 g          627 t
ORIGIN      
        1 gagttgacca cgcgagaagc cgacaacctg ttgaacaaac attcgggcgt gctcggcatc
       61 agcggcgaat caagcgatct gcgcgacctc gaggaagact ggccgaagaa gagtgagaaa
      121 gcaaacctcg cgctcgaagt ctatgcctat cgcattcgca agtatatcgg cgcctacatg
      181 gccgccctca atggttgcga tgccctgatc ttcaccgccg gcgttggcga gaattcgccg
      241 atcattcgcg gtatcgcgct caagaatctc gattttctgg gcatcaagat cgactatgac
      301 gtgaacgaac gcacctggcg cggtgccgag ggtgatatcg ccacgtctga ttcgaaggtg
      361 agagtcctgg tcattcccac caatgaagag ctggtgatcg cagaagacac cctcgatatt
      421 gtgaaaccta aaaagaagta agcacaggcg cgaaaacggc caccctgatg ggtggccgtt
      481 ttttcgttct aggcgggggc tggactatcg agctgagtcc ttgcgagacg acggctcgat
      541 ccgcaggtca ggcaagccga gattcgccgc caatcgtcgc agattcttcc actcgagacg
      601 cggatcaccg gacggatcct gtggggtttc gaggatcacc gtcattccct tcagcaggcg
      661 atgctgaagc aatgccgcta tggcggtgtc gccgatgtga ccttccccaa tgtgctgatg
      721 ccgatcgtgg cctttggcga acggattgag cgagtcgttc agatgcacca cgcgcacggc
      781 ttcaacgccc aggtgtttac gccacgcatc gaaaaagtcc tggcggccgt cgactgagac
      841 caggtcataa cccgcctgaa aggcatggca ggtgtcaaga cagacgccag tcggtagatc
      901 aactgccgcc cgaacccggg caatgtcttc gaggcgcgcg cccagatcgt ttcgctcgcc
      961 ggcggtgttt tccagcagta acagcggttt ttcgagagct ctgccgccga gggcttcgtt
     1021 gagcccgtcg atcatgagct tcaagccggg tttgtgttct gcatggctac ccatgtgctg
     1081 cacgacgtaa tcggcaccca gagctttggc caactggtac tcttcgcgca acaggtgaat
     1141 cgagccgtct ctgacttcgg ggcgcgcgga agccagattg atgagatacc cggtgtggac
     1201 aaccagaggg ggattgccga cggcttcccg ctgtgtctgg aactcgagca gatactctgg
     1261 cgacaagggc gtgcgcttcc agacccgggg actgccgacg aagatctgca gactctgaca
     1321 ggcccgggcg cgatagcgct caaacagcgc tttcggccca ccggccacac tcacatgaaa
     1381 cccgatcttc agatcgttcg gccacgcgac gaccgcggtt gtcctggttg cctttttcaa
     1441 ctcagctttt ctgttcatgg tttcaaacca caagagtgcc ttcgttaagc catgcctcgg
     1501 aacttcacgc actcggtgtt tcgctgacgg aaaaaagcgg caggctttcg cctgccgctt
     1561 atgttcacgc ccccacgggg attcgaaccc cggtcgcagc cttgaaaggg ctgtgtccta
     1621 ggcctctaga cgatgggggc aggatatcat tgcctggaat gtgggcccag cagggttcga
     1681 acctgcgacc agccggttat gagccggcag ctctgccact gagctatagg cccttaatgt
     1741 ctggttagtt taccatacca ggcagcgttt tgcaattact ccatgtaaaa ttctttcagt
     1801 tttttactgc gactcggatg ccgaagcttg cgtaacgcct tcgcttcgat ctgacgaatg
     1861 cgctcgcggg tgacgccgaa cttctgtccg acctcttcga gggtgcgctg ccagccatct
     1921 tcgagaccaa agcggaactt gatgaccttg gcttcgcgtt cggtcagcgt cgagagaaca
     1981 cgattgatct gctctttcag gatgttgttg aaggccgttt ccgccggcgg caacgcggac
     2041 ttatcctcaa cgaaatcacc caggtgcgaa tcttcttcgc cattgatggg cgtttcgaga
     2101 gagatcggat ccatcgccgt cttcatgatg ttcttcacct tctcgatcgg cagaccgact
     2161 tccttcgcca gttcctcgag taacggatcg cggcccatct tctgcaccag tttgcgactg
     2221 gctttgcgga tcttgttgat ggtttcgacc atgtgcacgg gaatgcggat cgtccgcgcc
     2281 tgatcagcaa tcgcgcgtgt gatcgcctga cgaatccacc agatggcata ggtcgagaac
     2341 ttgtaccctt tgcgatactt gaacttctcg acggcgcgaa tcagaccctg gtttccttcc
     2401 tgaatcaggt cgaggaacag tagaccgcgc ttcgtatact ttttcgctac ggagaccaca
     2461 agtcgcaggt tcgcttcaat cagcttccgc tgcgcttcct ggtcaccgtg ctcgatgcgc
     2521 tttgccagtt cgacttcttc ttgcgttgtc agcaggcgaa tcttgccgat ctcgcgcagg
     2581 tacacacgca cggaatcatc gatgtcatcg aagtcctcga cgtcaacatg cgcttcgtcc
     2641 ttcttgactt ccggcatgcg gagcttttcc ttgagccgga tctcatcgtc atcgacaagc
     2701 tcgacgttgc tttccatcaa ctcggtgatg atg
//
LOCUS       DAORHD010000629         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43903_length_2731_cov_3.190209, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000629 DAORHD010000000
VERSION     DAORHD010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43903_length_2731_cov_3.190209"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="PKO06_13220"
     CDS             complement(<1..1254)
                     /locus_tag="PKO06_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV70654.1"
                     /translation="MKDVPWHAIPVEETVQKLQTSVKTGLDPAEVDKRQATVGANVLT
                     PPPTVPAWRRFLAQFENPLIIILLVSAGACVLVGRITDALTILAVTIVNAVIGFVQEQ
                     NAQKAIDALAKMVKTECHVFRGSDIPVKIDAEGLVPGDLILLTSGDRVPADLRIIDSK
                     NLQINESALTGESLPVSKSTSPVPEDAGLGDRKCLAFSGALVTGGTGLGLVVATGDAT
                     QIGLINAMMNQTEEIDTPLMRRLEAFSSVFGYWVIGFSVTLMAIGIYHALRSTVPPFL
                     CEGPEQASLLQAFVLVKASVIAQLGGCPVSVPLYHSLSFVADVGDASFRLKIVEVFMA
                     AVSAAVGAIPEGLPAVMTIVLAIGTKNMAARKAIIRKLPAVETLGSVTVICSDKTGTL
                     TANEMTVQRILAGGKGFTVDGVGFNP"
     gene            complement(1292..1969)
                     /locus_tag="PKO06_13225"
     CDS             complement(1292..1969)
                     /locus_tag="PKO06_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70655.1"
                     /translation="MMFSIFLKAHADLREKSERQECLARRGITLIEILFVMSIITLLT
                     TTIYQVLRSVNTSYIHARNKLDILQTTRLIMAGLRNELRNAIDKPQLLNGQLNIPVAK
                     DKTIVYFFDEKTRRLYKGEKPDTNPDPLDPGEMKLFMFDDGQIMGFEYDSSYRDANAF
                     AESELALNSKVWCKVTMRILYTDKFKDLSDKEKEKIVTSTDDPRVKSFFMMISPRRIN
                     WLLQATQ"
     gene            complement(1969..2400)
                     /locus_tag="PKO06_13230"
     CDS             complement(1969..2400)
                     /locus_tag="PKO06_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70656.1"
                     /translation="MTQRRAFSLVEILIVVMLMSAGILPIYSLIKGGQKRIVRADTRT
                     LATLYGSSAIELARTLGYEKAGQLDKEKEFQELCENAQKNGFQILYQSKREKVLPSPP
                     GAQQQYLLRVDILVKAINRVISDVPELNFTTIITDPRVNFY"
     gene            complement(2413..>2731)
                     /locus_tag="PKO06_13235"
     CDS             complement(2413..>2731)
                     /locus_tag="PKO06_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70657.1"
                     /translation="MVTIIAPLIEINTDAAATAKCYVEANLISVLEPIRIKGKKELVI
                     RGTVASPNMNMKNCIQAPTTIKYNSLNAAWGIKPEWYDQMYVAKIVTGGVGKFDWTYV
                     QRQ"
BASE COUNT          643 a          758 c          700 g          630 t
ORIGIN      
        1 cggattgaag ccgactccgt cgacggtgaa acccttgcca ccagccagaa tacgctgcac
       61 cgtcatctcg ttcgcggtca gcgtccccgt cttgtccgaa cagatcaccg tcacactgcc
      121 cagagtttcg actgccggca acttgcgaat gatggccttt ctcgccgcca tgtttttagt
      181 accaatggca agcacgatcg tcatgactgc gggcaaccct tcgggaatcg cgccgacggc
      241 cgcgctcacg gctgccatga acacttcaac gatcttcagg cgaaacgacg cgtcgccgac
      301 gtcggcgacg aaggacaagc tgtgatacag cggcaccgat acggggcacc cgccaagttg
      361 agcgatcaca gaggctttca ccagaacaaa ggcctgcaac agcgatgcct gctccggccc
      421 ttcacacagg aaaggaggaa cggtgctgcg cagggcgtga tagattccga tcgccatcag
      481 cgtcacactg aaaccgatga cccagtagcc gaacacgctg gaaaacgctt cgagacgtcg
      541 catcagcggc gtatcgatct cttcggtctg attcatcatg gcgttgatca aaccaatctg
      601 cgtggcgtct ccggtggcca cgacgaggcc aagcccggtg ccgccggtga ccagcgcacc
      661 actgaacgcc aggcactttc ggtcacccaa accggcatct tctggaaccg gggatgtcga
      721 cttggaaacc ggcaggctct cgccggtcaa agcagactca ttgatctgca gattctttga
      781 gtcgatgatg cgcagatcgg cgggaactcg atctccactg gtcaacagga tcagatcacc
      841 gggaaccaaa ccttcggcat cgatttttac gggaatgtcg ctgccgcgaa acacgtggca
      901 ttccgtcttg accatcttgg ccagggcatc gatggctttc tgagcgttct gttcctgaac
      961 aaaaccaatg accgcattca cgatagtcac tgccaggatc gtcagcgcat ctgtgattct
     1021 gccgaccagg acgcaagcac cggcagagac cagcagaatg atgatcaatg gattctcgaa
     1081 ctgcgccaga aaccgtcgcc aggccgggac agtcggcggt ggcgtcagaa cattggcacc
     1141 gacggtcgcc tggcgtttgt cgacctcggc cgggtcgaga cctgtcttga ccgatgtctg
     1201 taacttctgc accgtctctt cgacgggaat agcatgccag ggaacgtctt tcacggaata
     1261 cgcactcctt cagaggtatg aatggttggt gttactgagt tgcctgcagc agccagttga
     1321 tacggcgtgg tgaaatcatc atgaaaaatg atttcacacg cggatcatcc gtgctggtca
     1381 cgattttttc cttttcttta tcggacagat ccttaaactt gtcagtatac agaatacgca
     1441 tcgtcacttt gcaccagacc ttgctgttga gtgcaagttc cgactcagcg aacgcattcg
     1501 catcacgata actcgaatca tactcgaacc ccatgatctg tccgtcatcg aacatgaaca
     1561 acttcatttc gcccggatca agcgggtcgg gattggtgtc gggtttctcg cccttgtaca
     1621 aacgcctcgt cttttcgtca aagaaataga caatcgtttt gtctttcgcg acgggaatgt
     1681 tcagctgccc gttcagcaac tgtggtttat cgatggcatt tcgcagttca ttgcgcaacc
     1741 ctgccatgat caggcgtgtg gtctgcagaa tgtcgagttt attgcgcgca tggatatagg
     1801 acgtattgac cgatctcaac acctggtaaa tcgtcgtcgt taacagggtg ataatgctca
     1861 tgacgaaaag aatctcgatc aacgtgattc ctcgccgagc gagacactcc tgccgttcag
     1921 acttctctct gagatcggca tgagctttca agaaaatgga aaacatcatc agtagaagtt
     1981 cactcgcgga tcggtaataa tggtggtgaa attgagttcg ggaacatccg aaatcacgcg
     2041 attaatcgct ttcaccaaga tatcgacccg caacagatac tgctgctggg ccccaggagg
     2101 gctgggaagc actttttcac gtttactctg atacaggatt tgaaagccgt ttttctgggc
     2161 attttcgcac agttcctgga actctttctc tttatcaagc tgtcctgctt tttcataacc
     2221 gagagtacga gccaactcaa tggcagatga gccatacaag gttgcgagcg tgcgggtgtc
     2281 tgcgcgaacg atacgtttct ggccgccctt gatcagagaa tagatcggaa gaattccggc
     2341 cgacatcaac atgacgacga tcagaatctc gaccaatgaa aacgcccgac gctgggtcac
     2401 aggaatattc acctactgac gctggacata ggtccagtcg aacttcccta cgccacccgt
     2461 cacgatcttg gcaacataca tctggtcata ccattccggc ttgatacccc aggcggcgtt
     2521 gagagaattg tattttatgg tggtcggagc ctgaatgcaa ttcttcatat tcatgttcgg
     2581 actcgcgacg gtaccgcgaa tgaccagctc ctttttgccc ttgatgcgaa tcggctcaag
     2641 aacgctgatc agattcgcct cgacataaca cttggccgtg gctgcggcat cggtattgat
     2701 ctcgatcaga ggggcaatga tcgtcaccat g
//
LOCUS       DAORHD010000630         2728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43973_length_2728_cov_3.715301, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000630 DAORHD010000000
VERSION     DAORHD010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43973_length_2728_cov_3.715301"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKO06_13240"
     CDS             <1..339
                     /locus_tag="PKO06_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tungsten ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV70658.1"
                     /translation="GGKMTGANYLESGQGMSEVLVMAGEKKAYTLTDRATWLTMRKKL
                     DLVLLYQNPPELKNSYAVIAVSNAKVPTAKTELAKTFVEWVTSPAAQSLIASYTLEGD
                     TLFYLSVGKR"
     gene            647..2530
                     /locus_tag="PKO06_13245"
     CDS             647..2530
                     /locus_tag="PKO06_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV70659.1"
                     /translation="MKLSHKLFLGFFALAFIVLVVGFVGWRSVGQLDESLTVIGSQRV
                     PALEHMGQIEKDLEAVRVAQRTLLSAKVAGEDRARQYRNIQAARERYLKAIEEYEKLP
                     LPKKEKDAFAAFTAVLGEWREENTKLFSLIKELETLDILDPDGLRAQVERFRGDHYRL
                     VAKVAEMVSIEKSFEGGDDHTACGFGKFVAGFKTSNPVLTKEVAAMAKAHAAFHAAVK
                     EARNAWKTGDKTRTSTILWQQLYPDAELVIAGFDNMNTEIAKAQKLFEQIYTLTMVNC
                     VEKQRKAVGALADAGKLNDEAVRAAVEEAHHDSTRAKQMSGGAIIGGLILAFLLAITI
                     TRSIAGPLAVAIDAMRTGSQQVASASAEISQTSQSLASGSTEQAASIEESSSALEELA
                     GQARNNAESAQKANDLMTATSQLMESNRQSMQQTVGTMGEIKSAADKVAGIIKTIEEI
                     AFQTNLLALNAAVEAARAGEHGKGFAVVAEEVRNLAQRSAVAAKDTAQLIGTSIQLSN
                     RGAEMVNKAAQGLETVVGNAAKVAEMVRGIDAASREQSQGVTQINEAVAQMDKATQQA
                     AAGAEEAAAASEELSAQAQEMLRVVAGLVLLVEGQNADTMLAEASPQPERHALATRKT
                     GKD"
BASE COUNT          646 a          706 c          837 g          539 t
ORIGIN      
        1 ggagggaaga tgaccggagc gaactacctg gagtctggac agggaatgag tgaggttctg
       61 gtcatggctg gtgaaaagaa agcctacacg ctcaccgatc gcgcgacctg gctgacgatg
      121 agaaagaaac tcgatctcgt gctgctctat cagaaccccc cggaactcaa gaactcgtac
      181 gcggtgattg ccgtcagcaa tgcaaaggtt ccgacggcca agacggagtt ggccaagacc
      241 ttcgtggagt gggtgaccag cccggccgca cagtcgttga ttgccagtta tacgctcgaa
      301 ggagacaccc tgttttatct ctcggtcggt aagcggtaac gtttgtgctg ctctgagaag
      361 cgtagatcgg acgacctcga caatcgggct ctgcaggctg cagagcccgg tgtttttagg
      421 agtttgttgg gtgaaccagt ggctccagtg ccggtctctg agatcgagga gtatgctggt
      481 gtcgcatgga caacacacga agcccgtgaa tcggaacaat actgacaact tgtccaagac
      541 aacgtgagca tgctaggata cgcctagaga tgagagcacc tttgcagttg cagaaatgat
      601 gcaggtttcg taaaaatcca gaccgcattc ttcgggggag gaattcatga agctcagtca
      661 caagttattt cttggctttt ttgccctggc gtttatcgtg ttggtggtcg gtttcgtggg
      721 ttggcgcagt gttggacaac tcgacgagag tctgaccgtc atcgggtctc aacgagttcc
      781 ggcactggag cacatgggac agattgagaa agatctcgaa gcggttcggg tcgctcagcg
      841 gacgcttctg agcgcgaaag tcgccggcga agaccgtgct cggcagtatc gaaatattca
      901 ggcagcccgc gaacgatacc tgaaggccat cgaagagtat gaaaaacttc ccttgccgaa
      961 aaaggagaaa gatgcgtttg ctgcgttcac cgcagtcttg ggagagtggc gcgaagaaaa
     1021 cacgaagctg ttcagcttga tcaaagagct cgaaacactg gatattctcg atccagatgg
     1081 tttgagggca caggtcgaga gatttcgtgg cgatcactat cgcctggttg ccaaggtcgc
     1141 tgaaatggtc agcatcgaaa agtcattcga aggtggcgat gaccacacgg cgtgcggttt
     1201 tggcaagttc gtcgccggtt tcaagacgag caatccggtt ttgaccaaag aagttgcggc
     1261 aatggccaag gctcatgcag cgtttcacgc ggccgtcaaa gaagctcgga acgcctggaa
     1321 aaccggtgac aaaacgcgaa cctcaacgat cctgtggcag cagctctatc ctgatgctga
     1381 gcttgtcatt gccggtttcg acaacatgaa taccgagatt gccaaagcgc agaagttgtt
     1441 cgagcagatc tatacgctga ccatggtcaa ctgtgtggaa aaacagcgaa aagccgtcgg
     1501 agcccttgcc gacgcaggaa aactgaacga tgaagccgtg cgcgctgcgg tcgaagaagc
     1561 ccatcatgac tcgacgcgtg ccaagcagat gtccggcggg gcgatcatcg gtggattgat
     1621 tctcgcgttc ttgctggcga ttaccatcac ccgctcgatt gcggggccgt tggccgtggc
     1681 tattgatgcc atgcgtaccg gcagtcaaca agtggcatcc gcatcagcag agatttcgca
     1741 gacaagtcaa tcgctggcat cgggttcgac ggaacaggcg gccagcatcg aagaatcgtc
     1801 gtcagcgctc gaagaacttg ctggtcaggc acgcaacaac gcggaatcgg cccagaaagc
     1861 caacgacctg atgacagcga cgagtcagct gatggaatca aatcgtcagt cgatgcaaca
     1921 gacggtcgga accatgggtg agatcaagtc cgccgcggac aaggtcgccg gcatcatcaa
     1981 gaccatcgaa gaaatcgcgt ttcagacgaa cctgttggca ttgaatgcgg ctgtcgaggc
     2041 agcacgtgcg ggcgaacacg gcaaaggctt tgccgtcgtc gcggaagagg tgcgtaatct
     2101 ggcgcagcgc tcggctgtcg ccgcgaaaga tacggcgcag ttgattggta ccagtattca
     2161 gctgtccaat cgcggcgcag aaatggtcaa caaggcggca cagggtctcg agactgtggt
     2221 cgggaatgcg gcaaaagtgg ccgagatggt gcgtggaatc gatgccgcct cacgtgagca
     2281 gtcacaaggg gtgactcaga tcaacgaggc tgtcgcccag atggataaag cgacgcagca
     2341 agccgcggct ggtgccgaag aagccgccgc cgccagcgag gaactgtcag cgcaggccca
     2401 ggaaatgctg cgcgtggtcg ccggcctggt actactcgtc gagggtcaga atgccgatac
     2461 catgctggca gaagcctctc cgcaacccga gcgccacgct ctggcaacca ggaagactgg
     2521 caaggactga gcttgctgtc gagctgacca cgggctcgcg tgcctcgtct tgccgaacaa
     2581 gtgaagaagc ccccggcgtt tgccgggggt ttcttcgctt gacgacgatc ttcgcatcag
     2641 ttgctgccgg aagagctttt catgcaggaa ccatcgcgtt tccgcaagcc gttgccgcgg
     2701 ccctgactct gtccctggcc ctgctttg
//
LOCUS       DAORHD010000631         2727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_43991_length_2727_cov_4.674401, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000631 DAORHD010000000
VERSION     DAORHD010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43991_length_2727_cov_4.674401"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2101)
                     /locus_tag="PKO06_13250"
     CDS             complement(<1..2101)
                     /locus_tag="PKO06_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70660.1"
                     /translation="MSTLHERQALLEMAMSIGNSLDREAMLKETLTVFLRALNGFAGA
                     VMRVHENGNELEFTLPRAAQRSSALGEAAEQAERRLRIGERFPEPLLTTPDGHMFYGW
                     MLGTNRCLIIGRSGVLPEHLVRELDLLARRLDYALVACLQFEQLRRQSQQELAQNREK
                     LAESEQRFRSLFELNPDGVLIVQDGVVIDANAAAREMLGASVEENLVGKRFSDIWGQI
                     SQEARAALDASMNDAGEQRQSCFEQDFLDLEGERRTAEVRLADLASSGPRLRQAIVRD
                     ITRRKLMETEVLRARDLAEAASAAKGEFLANMSHEIRTPLNGIIGMTGILLSSLSGEQ
                     RQYAELIQRSGDALLKVINDILDYSKIEARKLQLEQIDFHLPTMVDDTLELLALKAHE
                     KGLELTCSLAHDLPVDVCGDPGRLRQILLNLLGNAVKFTESGGVTLRVEREPEPETTS
                     AETESAGDGVLIRFSVVDSGIGIPAHQLGHLFSPFTQVDGSTTRRFGGTGLGLAISRQ
                     LSELMGGRIGVQSEHHVGSTFWFTIRLSVKSPQCDEVWQPLEMVAGKRVLILGSMKLV
                     HRQIEELLRHWQASCVRSESDDDALALERAAIGAEKPFDLILCDQLLNNGEGCEFFER
                     RRRERLPPVPGLILIPIGRNSNIGATARVMGVYPLMLPLRVEVLRQLLTQVCDPGVVA
                     AESLGGAAPPIAPLVAPV"
     gene            complement(2098..>2727)
                     /locus_tag="PKO06_13255"
     CDS             complement(2098..>2727)
                     /locus_tag="PKO06_13255"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV70661.1"
                     /translation="AMDAFTQLLEAYVSQRASPITDALAWSGMQAFCRGFTEVWRDGS
                     VATNGWSELAYAALCSGICLAQAGLGAVHGLAAGLGSRFDIPHGAACGTLLAAVTAAN
                     LRRARATAETGTLAKYAEVGRLVSQQSELDEVTAGDLLVKTVSDWVSRYELPLLATFG
                     VTEADLDDLIGRSRGSSMKTNPVVLSDGELRLILQSRLGQSDALESGPT"
BASE COUNT          550 a          872 c          779 g          526 t
ORIGIN      
        1 cgacaggcgc gacgagagga gcgatggggg gagctgcacc accgagggac tccgccgcga
       61 ccacgccggg atcacacacc tgagtcaata actgtcgcaa gacctcgaca cgcagaggaa
      121 gcatcagcgg atagacaccc atcactcgtg ccgtggcacc gatgttactg ttcctgccga
      181 tcgggatcag aatcaggccc ggaacgggcg gcagtcgttc cctgcggcga cgttcaaaga
      241 actcgcagcc ctctccattg ttcagaagct ggtcgcagag gatcaggtcg aatggttttt
      301 ctgcgccgat cgccgcgcgc tcgagtgcga gagcgtcgtc atccgactcg gatcggacgc
      361 aggatgcctg ccaatgccgc agcagttcct cgatctgtcg gtggaccagt ttcatggatc
      421 cgaggataag gacgcgtttg ccggcaacca tctcgagcgg ctgccacacc tcgtcgcact
      481 gcggcgactt gacggacaat cgaatggtga accagaaagt agaacccaca tgatgttcgc
      541 tttgaacacc gattcttccg cccatcaact cggacaattg tctggatatc gccagtccga
      601 gcccggtgcc gccgaatctg cgggtcgtcg aaccatccac ctgggtaaag ggcgaaaaga
      661 ggtgccccaa ctgatgagcc gggatgccaa tgcccgaatc cacgacggaa aagcggatca
      721 gaacaccgtc accggccgat tccgtttctg ccgatgttgt ctccggctca ggttcgcgct
      781 caactcgaag ggtgacacca ccggattccg tgaacttcac ggcattgccg agcaggttga
      841 gcagaatctg gcgcagcctg ccggggtcac cgcagacatc aaccggcaga tcatgagcga
      901 gcgagcacgt cagctcgagc cctttttcat gggccttgag cgccagcagt tccagcgtat
      961 cgtcgaccat ggtcggcaga tgaaaatcga tctgttcgag ttgcagctta cgagcttcaa
     1021 tcttggaata gtccaggatg tcattgatga ctttcagcag tgcatcgcca cttcgctgaa
     1081 tcagttccgc atactgccgc tgctccccgc tcaacgagga cagcagaatt ccggtcatgc
     1141 cgatgatgcc gttgagcggt gtgcgaatct cgtgactcat gttggccagg aactcgcctt
     1201 tggcggcgct ggccgcttcg gcaagatcgc gagcccgcag aacctcggtt tccatgagct
     1261 tgcgccgcgt gatgtcgcga acaatcgctt gtctcagtcg cgggccactc gacgccagat
     1321 cggcaagccg tacctcggcc gttcgtcgct cgccctcaag atcgaggaag tcttgctcaa
     1381 agcagctctg tctctgctcg ccagcatcgt tcatcgatgc gtcgagcgcc gctcgtgcct
     1441 cttgtgagat ctggccccag atgtcgctga accttttccc gaccagattc tcctcaacag
     1501 atgctcccag catttcgcgt gcagccgcgt tcgcgtcgat gacgaccccg tcctgaacga
     1561 tcaggacccc atcgggattg agttcgaaca acgatcggaa ccgctgctcg ctttccgcca
     1621 gtttttcccg gttctgcgcc aactcctgct gtgactggcg gcgcagctgc tcaaactgca
     1681 ggcaggcaac cagggcatag tcaagacgtc gcgccagcag gtcgagctcg cgcacgagat
     1741 gttcgggcag caccccggag cgaccgatga tcagacagcg attggttcct aacatccagc
     1801 cgtagaacat gtgaccgtcg ggggtggtca gcagcggctc aggaaaacgc tctccgatcc
     1861 tcagccgtcg ctcggcctgc tccgccgctt ctcccagcgc actgcttctc tgcgcggcgc
     1921 gtggaagcgt gaattcgagt tcgttcccgt tctcgtgcac acgcatgacg gccccggcga
     1981 aaccattcag agctcgcaga aagacggtga gcgtctcttt cagcatcgcc tcccggtcca
     2041 ggctgttgcc gatggacatg gccatttcga gcagggcttg tcgttcgtgc aacgtgctca
     2101 tgtcggtccg gactccagcg catccgactg tccgagtcga gactgcagaa taagcctcaa
     2161 ttcaccatca gaaagcacga ccggatttgt cttcatgctt gaaccgcgag acctgccgat
     2221 cagatcgtcc aggtcggcct cggtcacgcc gaaagtcgcc agcaagggca attcgtaccg
     2281 gctcacccaa tcagatactg ttttcacgag cagatctccg gcggtcacct catcgagttc
     2341 agactgctgc gacaccagtc ttccgacctc cgcatacttg gcaagcgttc ctgtctcggc
     2401 cgtggctctt gcccgacgca gattcgcggc ggtgacggcg gcgagcagag tcccgcaggc
     2461 agcgccgtgc gggatgtcaa atcgactgcc gagtccggcg gccagaccat gaaccgcacc
     2521 cagacccgcc tgagccaggc agatcccgga acagagggcg gcgtaggcca gctcagacca
     2581 accgttcgtt gcgaccgagc catcgcgcca cacttcggtg aacccgcggc agaacgcctg
     2641 cattcccgac caggccaacg cgtcggtgat cgggctcgcg cgttgagaga cgtacgcttc
     2701 cagcaactgt gtaaacgcat ccatggc
//
LOCUS       DAORHD010000632         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44027_length_2726_cov_3.245975, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000632 DAORHD010000000
VERSION     DAORHD010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44027_length_2726_cov_3.245975"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKO06_13260"
     CDS             <1..568
                     /locus_tag="PKO06_13260"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV70662.1"
                     /translation="RELSRIVSAKRFGKLRLRAMRILGAYGKSAGKALPTLTRLLLDA
                     NAEMRLTAAETILDLFASNSRKLPDVIRQLQDPKSPLRQAAVQLLQTAAPLAHPAAPL
                     LYEYLENGSIRPARAIGLLLPSTSLLRVFAEPLLEKADRVRLDPHAEATVEEESLENT
                     IAVETDAAQADAADQALSFEDIPAQELE"
     gene            598..1506
                     /gene="gnd"
                     /locus_tag="PKO06_13265"
     CDS             598..1506
                     /gene="gnd"
                     /locus_tag="PKO06_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HNV70663.1"
                     /translation="MHVGFIGLGKMGLNMVKRLSQGGHTVFGTARTAARRPELEQGGA
                     RWVDSLADFTRTLPAPRVVWVMVPAGQATEDTIQNLTHVLEPGDLIIDGGNSDYRDTQ
                     RRSEDLTRLGFRFLDSGTSGGIWGLKNGYCLMIGGLPRDVQIAAPLFDTLAPPQGWAH
                     VGPGGAGHMVKMVHNAIEYEMMQAMAEGFDVLDHSGMNLDLARIADLWNHGSVVRSWL
                     MELAASIFSENPKIEAIQGYVEDSGECRWTIETALQQGTPTPVFCASLHARYASRREN
                     AFANRFLAALRQKFGGHAVKPAESQE"
     gene            1503..>2726
                     /gene="zwf"
                     /locus_tag="PKO06_13270"
     CDS             1503..>2726
                     /gene="zwf"
                     /locus_tag="PKO06_13270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HNV70664.1"
                     /translation="MSLPGRPACTSSLVIFGGTGDLSRRKLIPALARLLAADLLPSDL
                     HLIITGRRPITHEQALQAIEAGLPTNRHSDVLLSRGLQHLLPRLHYLHLEPTQPESVT
                     TFFSELRSLEGQGAAPARLFYLSVPPDSIADFLPVLKTALDKTASTPCPSRVLVEKPF
                     GHDRASAQRINRQLLDIAREEQIFRIDHYLGKEAIQNILILRFANVFLEPLWNRQYID
                     HIQISFSETIGVGTRAGYFDQTGILRDVVQNHLLQVLCLIAMEAPNARHSDSIRQAKL
                     RILQALRPMSEETISDQVIRGQYAPGLLRGQTVAGYRSEHGVPAASQTETYIAMKLFI
                     DSWRWHQVPFYIRAGKRLAHDQTEVAVVFREAPPVALPAELGTPQPNILRIRVQPDES
                     IIMQVQSKAPGMQLAL"
BASE COUNT          560 a          853 c          752 g          561 t
ORIGIN      
        1 tcgtgagttg tcccgtatcg tatcggccaa gcgcttcggc aaacttcggc tgcgagccat
       61 gcgtattctg ggagcctacg ggaaatcagc cgggaaggca ctgccaacgc tcacccgact
      121 gcttctcgat gccaatgctg aaatgcgatt gaccgccgcc gaaaccattc tcgatctgtt
      181 tgccagcaat tcgcgaaaac tgcccgatgt gattcgccag ttacaagatc cgaagagtcc
      241 gctgcgtcaa gcagccgtgc aactgctgca aacggctgca ccactggcac atcccgcggc
      301 tccgctgctc tatgagtacc tcgaaaacgg ctctattcgc cctgcacgag ccatcggtct
      361 gctgttgcct tccacctcgc ttctgcgcgt gttcgccgaa ccccttctcg aaaaagccga
      421 ccgcgttcga ctcgatccgc atgccgaggc aactgtcgag gaagagtcgc tcgaaaacac
      481 catcgcggtt gagaccgatg ccgcgcaggc cgatgccgca gatcaggctc tgtcttttga
      541 agatatcccc gcgcaggaac tcgaatgaac cccaccacat cgaacaggag aaccaagatg
      601 cacgtcggct ttatcggtct cgggaaaatg ggtctgaaca tggtcaaacg cttgtctcag
      661 ggagggcaca ccgtgttcgg cactgctcgc accgcggcac gccgccctga actcgagcag
      721 ggtggtgctc gctgggtcga ctctctggct gattttaccc gcacccttcc tgcaccgcgc
      781 gtggtctggg tcatggttcc agccggccag gcgaccgaag acacgattca gaatctaacc
      841 catgttctcg agcctggaga cttgattatc gatggcggca actcggacta tcgtgacacc
      901 cagagacgat cggaagacct gacgcggctg gggttccgtt ttctcgacag cggtaccagt
      961 ggcggcatct ggggcctgaa aaatggatac tgcctgatga tcggcggact gccgcgcgat
     1021 gttcagatcg ccgctcctct attcgacacc ctcgccccgc cccaggggtg ggctcatgtt
     1081 ggcccgggag gagccggtca catggtgaag atggtgcaca acgccatcga gtacgaaatg
     1141 atgcaggcca tggccgaagg cttcgatgtt ctggatcaca gcggcatgaa tctggatctg
     1201 gcacgcattg cagatctctg gaatcatgga agcgtcgtgc gctcatggct catggagctt
     1261 gcagcctcca tcttctccga gaatccgaag atcgaggcta tccagggtta cgtggaagat
     1321 tccggcgagt gtcgttggac catcgaaacc gcgctgcagc agggcacacc gacccccgtg
     1381 ttctgtgcct cactccatgc gcgctatgct tcgcgtcgtg aaaacgcctt tgccaatcgt
     1441 tttcttgccg ccctcagaca gaagttcggt gggcacgccg tcaagccggc tgaaagccag
     1501 gaatgagtct tcccggacgc ccggcgtgca cctcgagtct ggtcatcttc ggtggaacgg
     1561 gtgacctgtc tcgccgcaag ctgattccag ccctggcgcg gcttctggct gccgacctgc
     1621 tgccttcaga cctgcatctg atcatcacgg gacggcgacc gatcacgcat gagcaggcac
     1681 tgcaggccat cgaagcgggt ttgccgacga accggcacag tgacgtgttg ctatcgcgcg
     1741 gcttgcagca cctgttgcca cgactgcatt atcttcacct cgagccaacc caaccggagt
     1801 ccgtcaccac gttcttcagc gaactccgtt cgctcgaagg tcagggcgca gctcccgccc
     1861 gcctgtttta cctttccgtg ccccctgatt cgatcgccga ctttctgccg gtattgaaaa
     1921 cggctctgga caagaccgct tcgacgccct gcccatcacg tgtgctggtg gagaagccgt
     1981 tcggacatga tcgtgcctca gcccagcgga tcaatcgaca gttgctcgat atcgcccgcg
     2041 aagaacagat ctttagaatt gaccattatc ttggcaagga agccatccag aatatcctga
     2101 ttctacgctt cgccaacgtc ttcctcgaac cgctctggaa caggcagtac atcgaccaca
     2161 tccagatcag tttctctgaa accatcggcg ttgggacacg cgccgggtac tttgatcaga
     2221 ctggaatcct gcgggatgtc gtgcagaacc atctgttgca ggtactctgt ctgatcgcca
     2281 tggaagcccc gaatgctcgg cattccgact cgatccgtca ggcgaaactg cggattctgc
     2341 aagccttgcg acccatgagc gaagaaacaa tctccgatca ggtgatccgc ggccaatacg
     2401 cccctgggtt gctgcgcgga caaaccgttg ccgggtatcg ctcagagcat ggggtgcccg
     2461 ctgcctcgca gacggaaacc tacattgcca tgaagctgtt catcgattcg tggcgttggc
     2521 accaggtgcc cttttacatc agggccggaa aacgactggc acatgatcag accgaagtgg
     2581 ccgtggtctt tcgtgaagcg ccacccgtgg cactgccggc agaactcggc accccccagc
     2641 cgaacatcct ccgaatccgc gtgcaacctg acgaatcgat catcatgcag gttcagagca
     2701 aagctccagg aatgcaactg gcgctc
//
LOCUS       DAORHD010000633         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44039_length_2726_cov_2.396481, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000633 DAORHD010000000
VERSION     DAORHD010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44039_length_2726_cov_2.396481"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PKO06_13275"
     CDS             complement(<1..997)
                     /locus_tag="PKO06_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV70665.1"
                     /translation="MEPGITGQDKGSQQQVLRYEYVLGALSDIGDELCRVTSFDAQLK
                     SLLHLLLGTLGVSKGGIFIYDSAASNLSLKCSWKLSKKALVHHIHRSNVEELEKVAKP
                     IFLPQTEVPFLDELFKGFATDDLSCFAILKVREKFLGLVVVGQKLQKSAFEERETEFL
                     STLSRNISVAINNFLLLNELRETNRRLDEKIQEVSILYQATQMIASEIQLQALLELAM
                     NLIIEISEGSRGSIWLFDEERNGLSRKSAFGSGDEPLEYVDLNDTQIGAKIVLEKEVL
                     IVNTDQPAPAPLSELDQKVFGTSFIILPILNQGEFLGFIHLTEKTNQTAFSERDQR"
     gene            complement(1011..1361)
                     /locus_tag="PKO06_13280"
     CDS             complement(1011..1361)
                     /locus_tag="PKO06_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV70666.1"
                     /translation="MAEEFSLDHHIDGPVLVIRTKGYLNDLGAEQVDTICSENLQKGT
                     NKIVINLEHSPLINSIGISILIGVIEAIEESNGALAFTNLTPTNRKTFEMMGLLQFAQ
                     AFDAEAEAVTKFKG"
     gene            1595..2380
                     /locus_tag="PKO06_13285"
     CDS             1595..2380
                     /locus_tag="PKO06_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70667.1"
                     /translation="MRWVSAGLIILLISGLIWLWTADAYGLELHQWAKLRHTKAQLWL
                     TDNPWMHLAIVGSCLLFLFLGISTILVLLPAMFFWPPWLGASLIMIVHLGLIMFKCRQ
                     RRLNPEQKLLPPALSERLPAIDAGIVGVSCLKLFLNLPGQAIDVLVLEKKTDSIPLEQ
                     TRLNLMVGTSLRVLVASVWAGSLISVVVNFHPFPERVLSFLLLGTAVIMWGLVWGYVP
                     ELLPGEKAVQETCKVLFNEPAATNSAVPPATQAQTAQQTNRPA"
     gene            2504..>2726
                     /locus_tag="PKO06_13290"
     CDS             2504..>2726
                     /locus_tag="PKO06_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNV70668.1"
                     /translation="MDTNTGMIKELKHRAARIRWLSIKTTADAGSGHPSTCCSAADIL
                     SALFFQVMRYDPQHPESAANDRLVLSKGHA"
BASE COUNT          589 a          733 c          721 g          683 t
ORIGIN      
        1 aacgctgatc tcgttcggaa aacgcggttt gattggtctt ctcggtcagg tggatgaatc
       61 cgaggaattc gccctgattc aaaatcggca gaataatgaa cgacgtgccg aacacctttt
      121 gatcgagctc gctgaggggc gcgggcgcgg gttgatcggt gttcacgatg agcacttctt
      181 tctcgagaac gatttttgct ccgatctgcg tgtcattcag gtcaacgtat tcgagcggct
      241 cgtctccgct gccgaaagcg cttttccggg agaggccgtt ccgttcttcg tcaaagagcc
      301 agatggaccc gcgactgcct tcggaaatct caatgatcag gttcattgcc agttcgagca
      361 gtgcctgcag ttgaatctca gaagcgatca tctgcgtagc ctgatacagg atgctgacct
      421 cctggatctt ttcgtccagg cggcgattgg tctcacgcag ttcgttcaac aacaagaagt
      481 tattgatggc gacagaaatg ttccgtgaca gggtcgacag aaactctgtt tcacgttctt
      541 caaaagcgct cttctgcagt ttctgcccga cgacgacgag accaagaaac ttttcgcgta
      601 ccttgagaat cgcgaaacag gagaggtcat cggtcgcgaa gcccttgaac agctcatcga
      661 ggaacggaac ttcggtttgc ggcagaaaga tcggcttggc aaccttttcg agttcttcga
      721 cattcgatcg gtgaatgtga tggaccagag ccttcttgga gagcttccag gagcatttga
      781 ggctcaggtt cgacgcggca ctgtcataaa tgaaaatacc gcccttgctg accccaagcg
      841 tgccaagaag cagatgcaga agagatttca gctgggcatc gaagctggtg acgcgacaca
      901 gttcgtcacc gatatcactc agggcgccaa gcacatactc gtaccgaagt acctgttgct
      961 ggcttccttt gtcctgaccg gtgatccctg gttccatgag gtggggaggc ctaccccttg
     1021 aacttggtta cggcttcagc ctcagcatca aacgcttgag cgaactgtaa aagccccatc
     1081 atctcgaagg tcttgcggtt tgtcggggtc agattcgtga aggcaagagc accattcgac
     1141 tcttcgatgg cctcaatgac gccaatcaga atcgagatgc caatggagtt gatcaatggc
     1201 gaatgttcga gattgatcac gatcttgttg gtacccttct ggagattttc cgaacagatg
     1261 gtatccacct gttcagcacc gaggtcgttc aggtacccct tcgtgcgaat caccagaacc
     1321 gggccatcga tatggtgatc tagagagaac tcttcagcca tgactggttt tatgaccccc
     1381 gaaattacgt tgtcttggag cgtgaaaaag tgtaacgcat ccttcgcaaa gaatcaacac
     1441 tcttttccca agaactataa ccatcgctac gttatcacac ttttttcgtt cccacaacca
     1501 ttccacctga aaaattggaa gaatcattcg cttgcctttt gacgaaacgt acatacttta
     1561 ttgtgccatg tttcggtaca ccacgtatga gcgcatgaga tgggtcagtg cagggctgat
     1621 cattttgctg atcagcggtc tgatctggct ctggacggct gacgcctacg ggcttgaact
     1681 ccatcagtgg gcaaagctcc gccacaccaa agcacagctc tggctcacgg acaacccctg
     1741 gatgcatctg gccattgtcg gctcctgcct gttgttcctg tttctcggca tttcgaccat
     1801 actggtcttg ctgccggcca tgtttttctg gccgccctgg cttggggcgt ctctgatcat
     1861 gatcgttcat ctgggtttga tcatgttcaa atgccgtcaa cggcggctga atccggaaca
     1921 gaagctgctc ccacccgcgt tgagcgaacg ccttcccgcc attgatgcgg gtattgttgg
     1981 cgtgtcgtgt ctgaagttgt ttctcaatct tccggggcag gcgatcgatg ttctagtcct
     2041 cgaaaagaaa accgactcca ttcccctcga acagacgcgc ctgaatctga tggtcggaac
     2101 gtcgttgcga gtgctcgtgg catcggtctg ggccgggagt ctgatctcgg tcgtggtcaa
     2161 tttccatccg tttcccgagc gggttctttc ttttctcctg cttggaacgg cggtcatcat
     2221 gtggggtctc gtctggggat acgttcctga actcctgcca ggtgaaaagg cggttcagga
     2281 gacgtgcaaa gtgttgttca acgaaccggc tgcgacgaac tccgcagtgc cgcctgcgac
     2341 tcaggcgcag actgcgcaac agacgaatcg gccggcctga ccacgcatcg aggccggtcg
     2401 agattcctgg cgcacggaat cttgtctctg gatccacctt ttccttttcc gctggggtac
     2461 gatagaatag tgccactttc gatacctctt ctctaggaga cctatggata cgaacactgg
     2521 aatgatcaag gaactcaagc atcgggcggc acgaatccgc tggctgtcca tcaaaacaac
     2581 cgctgacgca ggatcggggc atccttcgac ctgttgttcg gcggcagata ttctctcggc
     2641 acttttcttc caggtcatgc gctacgatcc tcagcacccg gaatcagcgg ccaatgatcg
     2701 tctggtcctt tccaaggggc atgcgg
//
LOCUS       DAORHD010000634         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44059_length_2725_cov_3.471161, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000634 DAORHD010000000
VERSION     DAORHD010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44059_length_2725_cov_3.471161"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKO06_13295"
     CDS             <1..249
                     /locus_tag="PKO06_13295"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNV70669.1"
                     /translation="VRIVADRPDRVRRLMQEQNLDEEKAGALIDQRDESAQKFLSDFF
                     DADWNDPHLYHLVINTSRVPLDQAVQMILRLSEACKTA"
     gene            246..1424
                     /locus_tag="PKO06_13300"
     CDS             246..1424
                     /locus_tag="PKO06_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV70670.1"
                     /translation="MSRRLLGLLLFLAGLATFIALQHPIESPSLLDTHIGAPVRFAPI
                     VGNDRAYVVTDQALICLASSGQPLFRTDLPESICGPVFVATDQLAILTRSELRLIHAE
                     RGETLWQIPHDFTANVRLAAQNGRLLLHDDRRAMVFTRESRPAGRWEAVGSLTQALLP
                     RDDVLVTLTSTLNASGTAVGRLSAYRLSTKSDIWSYSEDLTLDEFRYDNGQVYACTAA
                     GQPVLIDVETGNELYCVKRDGYRLLVQTPSCLILLVAGGSRIEIQPLSSGGAAWSVNS
                     SSPIQCGFVLRDDLVILNQDAARIFGIHDGNLKRQQQPGSALVSGGSLGAERLWLSYD
                     DSLWWKTRYCSAYVPKHPKPLWTAGETKEGVWPPVMLSSGDLLVSRSGRIRLFAHSGS
                     "
     gene            1553..>2725
                     /locus_tag="PKO06_13305"
     CDS             1553..>2725
                     /locus_tag="PKO06_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNV70671.1"
                     /translation="MQFRDLTIGFDIGSVSINTIICDGKGDIIEELPYQRHFGKTVGH
                     CAAILADVERKYTPEAIARVVFTGTHGETFAKALKTHFEVETTAQTNGLFKVFPQART
                     VVSIGGHDSALLLVNAGDRGFFLEDFRLNGACAAGTGSFIDQQAERIFADKPEFADIS
                     DPQERIEAILGHFIFEGSRSDAPANVACRCTVFTKSDMIHLQNKGIAIKHIIAGLHEG
                     VAKNFKSTLITSSTIQGPVAFIGGYATNELARTSFEKILGLPVTVPPHHTAIGAIGVA
                     QSGIRDNVGRAVRAAEIEQLSSSAVFAAVTTPPLCLKQSTFTPCGEFPGLPKGKGKIP
                     VYFGFDIGSTTTKLVLISPKGDIYYKRYMPTEGQPVVAIKKGLRHLQSNIDVKRLEI"
BASE COUNT          553 a          837 c          756 g          579 t
ORIGIN      
        1 gttcgcatcg tcgctgatcg gcctgatcgc gtgcggcgcc tgatgcagga acagaatctc
       61 gacgaggaaa aagccggcgc actcatcgat caacgcgatg agtcggcgca gaagttcctc
      121 agcgactttt ttgatgcgga ctggaacgat ccgcacctct accatctggt catcaatacc
      181 agccgtgtgc cgctcgatca ggcggtacag atgattctcc gattgtccga ggcctgcaaa
      241 acggcatgag tcggcgactt ctcggccttc tccttttcct cgccggtctg gcgaccttca
      301 ttgcgctgca gcatcccatc gaaagcccat cgctgctcga tacccacata ggtgcgcccg
      361 tcagattcgc tccgattgtc ggcaatgatc gcgcctacgt cgtcaccgat caggcactga
      421 tctgtctggc ttccagcgga caacctcttt ttcgcaccga tcttccggaa tcaatctgtg
      481 gacccgtgtt tgtcgccacc gatcaactgg cgattctgac ccggagcgag ctcaggctca
      541 ttcacgcaga acgcggggaa accctgtggc agattccaca cgatttcacc gccaacgttc
      601 gcctggccgc gcaaaatggc cgactgctgc tccatgatga ccggcgggcc atggttttca
      661 ccagagaatc gagaccagca gggcgttggg aagccgtggg cagtctgacc caggcactgt
      721 taccccgaga cgatgttctc gtgactctga cgagcactct caatgcctcg ggaacagccg
      781 tcggtcgatt gtctgcctat cggctctcaa cgaaatcaga tatctggagc tattcggaag
      841 atctgacact cgatgagttc cgctacgaca acggacaggt ctacgcctgc acggctgccg
      901 gacaacccgt gttgatcgac gtcgaaacag ggaacgagtt gtattgtgtc aagagagacg
      961 gatatcgcct gctggtccaa acgccttcct gcctgattct tctggttgcc ggaggctcac
     1021 gcatcgagat ccagcccttg tcttcgggtg gagccgcctg gagcgtcaac tcgtcgagtc
     1081 cgattcagtg cggattcgtt ctccgcgatg acctcgtgat tctcaatcag gatgcggctc
     1141 gcatcttcgg gatccatgac ggaaatctga aacgtcagca acagccgggc tcagccttag
     1201 tcagcggcgg atcactcggg gcagaacgcc tctggttgtc gtatgatgat tcactctggt
     1261 ggaaaacccg ctactgttcg gcatatgtcc cgaaacaccc gaagccgctc tggaccgcag
     1321 gcgaaaccaa agaaggcgtc tggccaccgg tcatgctcag cagtggcgat ctcctggtca
     1381 gccgcagcgg tcgcattcgc ctgtttgccc attcaggatc ctgagcctgc cgtcgtactg
     1441 cccggggaaa ccttcgcgaa tatgacccgg ttttggtggt tttcagccat tgccctgcgg
     1501 tcgaaaccgt ggtagacttg cccttctttt aatcgctcga ggagggttgt ccatgcagtt
     1561 tcgcgacctg acgatcgggt tcgacatcgg ctcggtcagc atcaatacca tcatctgtga
     1621 cggcaagggt gacatcatcg aagagttgcc ctatcagcgg catttcggca agacggtcgg
     1681 ccactgcgcc gccattctgg ccgacgtcga gcggaaatac actcccgaag ccattgcgcg
     1741 cgtggtgttt accggtacgc acggcgaaac gtttgccaag gctctcaaaa cccacttcga
     1801 ggtcgagacg accgcgcaaa ccaatggtct gttcaaggtg tttccacagg cgcgtaccgt
     1861 cgtctcgatc ggcggtcatg actcggcgct tctgctcgtc aacgcgggcg atcgcggttt
     1921 ctttctcgaa gactttcgcc tgaacggggc ctgcgcggcc ggtaccggca gcttcattga
     1981 ccagcaggcc gagcgtatct tcgcggacaa acccgagttt gccgacatct ctgacccgca
     2041 agagcgtatc gaagcgattc tcgggcactt catcttcgaa gggtcgcgtt ccgatgcgcc
     2101 cgccaacgtt gcctgccgct gcacggtgtt caccaagtcc gacatgatac acctgcagaa
     2161 caagggcatc gcgatcaaac acatcattgc cggcctgcac gaaggcgtgg ccaagaactt
     2221 caagtcgacg ctgatcacca gcagcacgat tcaagggccg gtcgccttca tcggcggcta
     2281 tgccaccaac gaactggctc ggacatcgtt cgagaagatt ctcggcctgc cggtgaccgt
     2341 gccgccgcat cacactgcga tcggcgcgat tggcgtcgca caatcaggca ttcgtgacaa
     2401 cgtcggccga gccgtgcggg ccgccgagat cgaacaactg tcaagttctg ccgtgttcgc
     2461 ggccgttacg actccgccgc tctgcctgaa gcagtcgacg ttcacgccgt gcggcgagtt
     2521 tcccgggtta cccaagggta aaggaaagat tcccgtctac ttcggattcg atatcggttc
     2581 gaccacgacc aaactcgtac tgatttctcc gaagggtgat atctactaca agcgctacat
     2641 gcccaccgag ggccaaccgg tcgtcgcgat caagaagggg ttgcggcatc tgcagtcgaa
     2701 catcgacgtc aaacgcctcg agatt
//
LOCUS       DAORHD010000635         2724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44077_length_2724_cov_3.926939, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000635 DAORHD010000000
VERSION     DAORHD010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44077_length_2724_cov_3.926939"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2724)
                     /locus_tag="PKO06_13310"
     CDS             complement(<1..>2724)
                     /locus_tag="PKO06_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV70672.1"
                     /translation="RIMRGESFQNIARTLFDQACRQTGATSGYVALLSDDGTENPVLF
                     LEAGGLPCTVDPTLPMPIRGLREQAYATGRAVCENDFMNSRWVSMMPAGHVVLKNVLF
                     APLNIDGQTVGIMGLANKPTDFTSENLHTAEVIGDLAALALSHWRKSEALKKLADDYQ
                     TLFREMLDGFALHEIICDTAGTPVDYRFLAVNPAFERMTGLKAEGLIGRTVLEVMPTT
                     EKHWISTYGRVALTGEPVHFHNVSGILGKVFEVTAFRPEPGKFACIFTDITAHQKAEQ
                     DLTSTRDFLENLINHANAPIIVWDPAFRITRFNQAFEQLTGHSAVEVIGKHISLLFPP
                     DRVEASMRLIAKTLTGERWESVEIQILHASGTISTLLWNSATIVDPATKVPAATIAQG
                     HDITIRLQKVQELRAAHSRLEALWSISSLADADVKKVSDHILKSLGSMTGSAYGFYGF
                     VSPDEQVMTIHAWSGEAMKDCSMVTRPSRFDVCTSGIWAEAIRRREPLIVNDYEAAHP
                     AKKGLPEGHVRLRNLLVVPFLSQDRITAVAAVANRPTDYTQDDISQITAFLNSIQALT
                     ESKRTEVALRESEERYRTVANFTYDWEYWLSPDFTFLYCSPACERITGYGAREFEQNP
                     RLLIELIHPDDRPRVDHHFSPNVCEQVEHHTDEFRIVTKTGEIRWIGHVCQMVRDSTG
                     SFRGRRASNRDITEQKLLHQQLLQAQKLESIGNLAGGVAHDFNNMLGVIIGRTENALA
                     QTPHDSPVFHDLEEIMRSARHSAELTGQLLAFARKQSATPVVIDLNVVVQGTLRLLRR
                     LIGENIRLDWNPASDVWPIKIDPGQIDQILTNLAINARDAIAGSGIIRLKTSNCTLKT
                     APSPLTGKFVPGDYVCFELSDNGCGMSQSIRERIFEPFFTTKELGKGTGLGL"
BASE COUNT          529 a          743 c          874 g          578 t
ORIGIN      
        1 caggccgagg ccggtgccct tgcccagctc cttggtggtg aagaacggct cgaaaatgcg
       61 ctcgcgaatc gattgcgaca tgccgcaccc attgtcagaa agctcaaaac acacatagtc
      121 gcctgggacg aactttccgg tgagcgggct cggggctgtt ttcaaggtgc agttacttgt
      181 cttcagacgg atgatgccgg aacccgcaat ggcgtctcgg gcgttgatcg ccagattggt
      241 caaaatctga tcgatttgtc caggatcgat tttaatcggc cagacatctg atgctggatt
      301 ccagtcgagt cgaatgttct cgccgatcag tcggcgcagc aggcgcaggg ttccctgaac
      361 gaccacgttc aaatcgatca ccacgggggt tgcgctctgc ttgcgggcaa aggcgagcag
      421 ttgtccggtc agttccgccg agtggcgggc cgaccgcatg atctcttcga ggtcgtggaa
      481 caccggcgaa tcgtgaggtg tctgagccag ggcgttttcg gtgcgtccga tgatgacacc
      541 gagcatgtta ttgaaatcat gggcgacccc gccggccagg ttgccgatcg actcgagttt
      601 ctgggcctgc aacaactgct ggtgcagcag tttctgctcg gtgatatcgc gattgctggc
      661 gcggcgccct cgaaatgacc ccgtactgtc gcgcaccatc tggcagacgt ggccgatcca
      721 gcggatctct ccggttttgg tgacgatacg aaactcgtcg gtatggtgtt cgacctgctc
      781 acagacattc ggagaaaaat ggtggtcgac ccgtgggcgg tcatccggat gaatgagttc
      841 gatcaataga cgcgggttct gttcaaactc tcgagcccca tacccggtta tgcgttcgca
      901 cgccggtgaa cagtagagga aggtgaaatc gggactgagc cagtattccc agtcataggt
      961 gaagttcgct accgtgcggt agcgttcttc gctttcgcgc agggccactt cggtacgctt
     1021 gctctcagtg agcgcttgaa tgctgttcag aaaggcggtg atctgactga tgtcgtcctg
     1081 ggtgtagtcc gtgggacggt tggcgacggc cgccaccgcg gtaatgcgat cctgcgacag
     1141 aaatggaacg accagcagat tgcgcaggcg cacatgcccc tcggggagcc ccttcttggc
     1201 cgggtgtgcc gcttcgtaat cgttgacgat cagcggctca cgccgtctga ttgcttcagc
     1261 ccagatgccc gacgtgcaga cgtcgaaacg tgacgggcgg gtgaccatcg aacaatcctt
     1321 catggcttct ccggaccagg cgtgaatggt catgacctgt tcgtcggggc tgacgaaacc
     1381 gtaaaagcca tacgcgctgc cggtcatcga gccgagagat ttcagaatgt ggtccgaaac
     1441 ttttttgacg tcggcgtcgg cgagagatga gatgctccag agcgcttcaa ggcgggaatg
     1501 cgcggcccga agttcctgaa ccttctgcaa gcgaatcgtg atgtcatggc cctgggcgat
     1561 tgtcgctgcc ggtactttcg tcgcggggtc gacaatggtc gctgagttcc agagcagggt
     1621 ggagatcgtt cccgatgcat gcagaatttg aatctcgacc gattcccagc gctcgccggt
     1681 gagtgtcttc gcgatcagac gcatcgaggc ctcgacgcgg tcaggcggaa acagcaggct
     1741 gatgtgtttg ccgatcacct cgacggcgga gtgcccggtg agttgctcga aagcctggtt
     1801 gaagcgggtg atgcggaagg cgggatccca gacgatgatc ggggcgttgg cgtgattgat
     1861 gaggttttcg agaaaatcgc gtgtgctggt caggtcctgc tcggctttct ggtgggccgt
     1921 gatatcggtg aagatgcagg cgaacttgcc cggttcggga cggaaggcgg tcacttcaaa
     1981 aaccttgccc aggattccgg aaacattgtg aaagtgcacc ggctcaccgg tcagggccac
     2041 gcgaccgtag gttgagatcc agtgcttttc ggtggtcggc atgacctcga gaaccgttcg
     2101 accgatcaga ccctcggctt tcagaccggt catgcgttcg aaggcggggt tgaccgcgag
     2161 aaaacgatag tcgaccggtg ttccagcggt atcgcagatg atctcgtgca gagcgaagcc
     2221 gtcgagcatc tcgcgaaaca gggtctgata atcgtcggcg agcttcttca gggcttcgga
     2281 cttgcgccag tgactcagag ccagtgcggc gagatcgccg atgacctcgg cggtgtgcag
     2341 attttcgctg gtgaaatcgg tcggcttgtt ggccagtccc atgatgccga ccgtctggcc
     2401 gtcgatgttg agcggagcga agagcacgtt cttcaatacc acgtggcccg cgggcatcat
     2461 cgagacccag cggctgttca tgaaatcgtt ctcacagacg gcgcgcccgg tcgcatacgc
     2521 ctgctcgcgc aacccgcgaa tcggcatggg aagcgtcgga tcgacggtgc agggcagtcc
     2581 gcccgcttcg agaaacagca ccgggttttc ggtgccatcg tcgctgagca gggcgacata
     2641 ccccgaggtg gcgccggtct gccgacaggc ctgatcaaag agggttcggg cgatgttctg
     2701 aaacgactcg ccccgcatga tgcg
//
LOCUS       DAORHD010000636         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44172_length_2720_cov_4.065291, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000636 DAORHD010000000
VERSION     DAORHD010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44172_length_2720_cov_4.065291"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PKO06_13315"
     CDS             <1..1545
                     /locus_tag="PKO06_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70673.1"
                     /translation="RDQRNAGSDLMRVICTQGDFGYFIAGFLLREFGVRVAPTLNDPL
                     VMRFEPSLMGGDPERRQAGAALRKLCEALRLHDLGALCRHLTNPALDEPLIIEDFRTD
                     PRERMQAGALVPERVAFLGHFIDPTFLSRHEPSLSRYTPDQLDVLIRRTWDSVRPCPL
                     VRRDIRSTFGHAVGFHFIGLLVTSEILFELLRRHEIERARNLIRESVIVARDHGCRLV
                     GFGQFTSIVTRNCKELVVPGVGFTSGNALTTGMALQALREVLKRRGKNLKDLHVAVIG
                     AGGNIGSTFARLLADEARVLTLIGGTGQDSRSRIEQTAGTILLDQSRQLRLGLTIGPS
                     GERLRSALPPSRLPQRAEGDPADILREGGIIWRSLQSHFTSSDSLRLGSLDDCRQADV
                     IVTATNATRPILHDDLVGQGAVICDISIPSAVAADVRARSDVTVFQGGVVALPHGEKL
                     NIGGLPLEPGLVYACMAETILLGLERRWDDYSVGDISKEQVLEITTIAERNGFRLGRI
                     KEEVSL"
     gene            1587..2225
                     /locus_tag="PKO06_13320"
     CDS             1587..2225
                     /locus_tag="PKO06_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HNV70674.1"
                     /translation="MTKHIDPRTWERRDQFDFFMAFDQPFFNVCTEVRLTNLRTAARQ
                     SGSSIFLAFLHAALTAAHQVPAFRQRLDGDQVVEYDRVHTAVTVRSAHDTFRFCWLDY
                     VPEFAPFQQAARERMEKAAQETGPLEPHGGRTDVIHVSSLPWIRFTSLMHPRKSGKSD
                     SVPKLVFGRFFEQNGEVLLPLSIEVHHALADGIHIAHFLDSYQSILDHPQSY"
     gene            2231..>2720
                     /locus_tag="PKO06_13325"
     CDS             2231..>2720
                     /locus_tag="PKO06_13325"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV70675.1"
                     /translation="MTSSSSNQAAATFNVSHPCLDCAVSCCQAFRIYVTAFDLSRISQ
                     RLGVAPSEFCLPVQCAGDNLDHRHLAFSLGAEEHFLLGLRRRRQGCLFLMRIGHQRRC
                     GIHDVRPLVCRSYPLAPYRQKPVMARQALCPRPWVLNDAQTQAFLRLQEQREREGCLY
                     QAL"
BASE COUNT          487 a          875 c          766 g          592 t
ORIGIN      
        1 cgcgatcagc gaaacgccgg atctgatctc atgcgcgtta tctgcaccca gggagatttc
       61 ggctacttca tcgccggctt tctgttgcgt gagttcggag tccgcgtcgc ccccaccctg
      121 aacgacccac tcgtgatgcg cttcgaacct tctcttatgg gcggagaccc cgagcgacgt
      181 caggccggcg ctgcattacg caagctctgc gaagcgcttc gtctacacga tctgggcgct
      241 ctctgtcgcc atctgacgaa ccccgctctc gacgaacccc tgatcattga agattttcga
      301 accgacccgc gagaacgcat gcaggcaggc gcactggttc ccgagcgggt ggcatttctc
      361 gggcacttca tcgatcccac gttcctgagt cggcacgagc cgtctctgag ccggtatact
      421 cctgatcaac tcgacgtctt gattcgtcgc acctgggact cggttcgccc ctgtcccctg
      481 gtccgccgag acattcgttc gacgttcgga cacgccgtcg gttttcattt catcggcctg
      541 ctggtgacct cggagattct gttcgaactc ctgcgtcgtc acgagatcga acgagcgaga
      601 aacctgatcc gcgaatcggt tattgtcgca cgtgatcacg gttgtcgtct cgtcgggttt
      661 ggacagttca cttcaattgt cacgcgcaac tgcaaggaac tggtggtgcc gggggtgggt
      721 ttcacgtccg ggaacgccct gacgaccggc atggccctgc aggccttacg cgaggtgttg
      781 aagcgtcgcg gcaagaacct gaaggatctg cacgtcgcag tcatcggtgc cggcggcaac
      841 atcggttcga cctttgcccg cctgctggcg gacgaagcac gggttctgac gcttatcggc
      901 ggcaccgggc aggattcacg ttcacgtatc gagcagacag ccggtacgat tctgctcgac
      961 cagagtcgac aactgcgtct cgggctcacc atcgggccaa gcggcgagcg gcttcgcagc
     1021 gcgcttcctc cctctcgtct gccgcaacgc gccgaaggcg accctgccga cattctgcgt
     1081 gaaggcggta tcatctggcg cagcctgcaa tcacacttca cctcatctga ttcgctgcgt
     1141 ctgggcagcc tcgacgactg tcggcaggcg gatgtcatcg tcacggcgac gaacgcgaca
     1201 cggcccatcc tgcacgacga tctggtgggg cagggcgccg tcatctgcga catcagtatc
     1261 ccttccgccg tcgccgccga cgtacgcgcc cgatcagacg tcacggtgtt tcagggcggt
     1321 gtcgtcgcct tgccgcacgg cgagaagctc aacatcggcg gcctgccgct tgaacccggg
     1381 ctcgtgtatg cctgtatggc tgaaacgatc ctgctcggac tcgaacgtcg ctgggacgat
     1441 tactcggtcg gcgatatctc gaaagagcag gtgctcgaga tcaccacaat cgccgaacgg
     1501 aatggattcc gcctgggacg catcaaggaa gaagtttctc tctgaacgcc tgacgttgcc
     1561 tgccacactc actccggagc acacgcatga cgaaacacat cgatccacgc acgtgggaac
     1621 ggcgcgacca gttcgacttc ttcatggcct tcgaccaacc cttcttcaat gtctgcaccg
     1681 aagtacgact gaccaacctc cgcactgccg cgcgccaatc tggcagttcg atttttctcg
     1741 cttttctcca tgccgcgctg accgctgccc atcaggttcc ggcatttcgg cagcgtctcg
     1801 atggcgacca ggtcgtggag tatgaccgtg ttcataccgc cgtcacggtg cgcagcgccc
     1861 acgacacatt caggttctgc tggcttgatt acgtccccga gtttgcgcca tttcagcagg
     1921 cggcacgcga gcgtatggaa aaagccgctc aggaaaccgg cccgctcgaa ccgcacggtg
     1981 gtcgaaccga cgtgattcac gtctcgagcc tcccctggat ccgctttacc agcctgatgc
     2041 atccgcgaaa gtcagggaaa tctgattccg tccccaaact ggtgttcggt cggtttttcg
     2101 aacagaatgg tgaagtgttg ctgcccctgt cgatcgaagt gcatcatgcg ttggccgacg
     2161 gcatccacat cgctcacttt ctcgattcgt accagtcgat tctcgatcat ccgcagtcct
     2221 attaagcaga atgacatcaa gctcttcgaa tcaggctgct gccacgttca atgtttcgca
     2281 cccctgcctg gactgcgcgg tctcctgctg ccaggcgttt cgcatctacg tgaccgcgtt
     2341 cgatctttcg cgcatttctc aacggcttgg agtggcacct tcggagtttt gtcttccggt
     2401 acagtgcgcc ggggacaacc ttgatcatcg gcatctcgcg ttttcgctgg gcgccgaaga
     2461 acactttctg ctcggcctgc gacgtcgacg ccaagggtgc ctcttcctga tgcgcatcgg
     2521 gcaccagcgc cgatgcggca tccatgatgt tcggcctctg gtctgtcgca gttacccgct
     2581 cgcgccgtat cgccagaaac cggtcatggc tcgacaggct ctgtgcccca ggccctgggt
     2641 gttgaacgat gcacagacac aggcttttct gcgtctgcag gaacagcggg aacgcgaggg
     2701 gtgtctatac caggccctgc
//
LOCUS       DAORHD010000637         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44219_length_2718_cov_3.724747, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000637 DAORHD010000000
VERSION     DAORHD010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44219_length_2718_cov_3.724747"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /gene="dnaX"
                     /locus_tag="PKO06_13330"
     CDS             <1..1529
                     /gene="dnaX"
                     /locus_tag="PKO06_13330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNV70676.1"
                     /translation="FMDIVEIDAASNNGVDDIREMREKVKYLPVEGKYKVYIIDEVHM
                     LSGAAFNAFLKTLEEPPPRVVFVLATTDPQKVPATILSRCQCFEFHSIPRKVLVERLS
                     EVVQRERTTDPGFPAVGQETLQLLAESASGGFRDALSLLDQVASANNGGEVSVEQVLQ
                     LTRRVGHTTLRKLAGVLFEGDVASVLRQLHELFFRGYEVQSLGKDLLEWLRRAVFLHV
                     DPQANQVLELPAEQAKDTLQLVQPLPLEYLMSVTSHLERAMTALRHSVHPRLLFELEL
                     VRLARREVSLGLEGTERRLSQVEQQLRTPRLVARPVGAAPTVVGTAEPGLRRADAAAP
                     MRPLPVKTETAVRSQPARPYEAQPKPNLTLVKREPVAGSPEHLSPTERWAAFRQEILT
                     CSTSTGSILEMARYEGFKDGVVTIVFGNDFSARRFREPSVLESIQPILEQFFGSGTRL
                     DVRGATSPSPVAVKESVTQVKENIAKIDQSVKDKILSQSTVTDTLAVFGGEIVGIEKK
                     "
     gene            complement(1905..>2718)
                     /locus_tag="PKO06_13335"
     CDS             complement(1905..>2718)
                     /locus_tag="PKO06_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006321983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNV70677.1"
                     /translation="IPAQISTKESFCSSSSTSPIAEGEAFELDVCGLQCPGPLLKMRE
                     RMEELKPGQVLKIVATDPGFPPDAASWAKSSGNKLLKAFPDKGRFTVLMEKTAEALSP
                     AVACRTQPMTKAEKALTMVVFSADLDRAYAVFIIANGAAAMGMKVTLFFTFWGLNILR
                     KREAPAVPKTALEQMFGMMMPKGPEALGLSKMHFAGMGTMMMKNVMAQKQVEPLPDLI
                     AKARQAGVRMVACSMSMDVMGIKHEELIDGIEIGGVASYIGSASDAEINLFV"
BASE COUNT          627 a          692 c          775 g          624 t
ORIGIN      
        1 atttcatgga tattgtcgaa atcgatgccg ccagcaataa cggcgtcgac gacattcgtg
       61 agatgcgcga aaaggtcaag tatctgccgg tcgagggaaa gtacaaggtc tatattatcg
      121 atgaagtcca tatgctctca ggagcagctt tcaatgcttt tctgaagacc ctcgaagaac
      181 caccgccacg cgtggtgttt gtgctggcaa cgactgatcc gcagaaagtg ccggcgacca
      241 ttctgagtcg gtgtcagtgt tttgagtttc attcgattcc ccgaaaagtt cttgtggagc
      301 gactgtccga ggtcgtgcaa cgagagcgaa cgacagaccc gggtttcccc gccgtgggtc
      361 aggagacgct gcaactgctt gccgaatcag catcaggcgg gtttcgcgat gcgctgagtc
      421 tgctcgatca ggtggcttcg gcaaacaacg ggggagaagt gtcggttgaa caggtcctgc
      481 agctgacgcg tcgggttggc cataccacct tgagaaagct ggcgggggtg ctgttcgagg
      541 gtgatgtggc atcagtgctg cgacaactcc atgaactctt tttccgcgga tatgaagtac
      601 aatctctcgg gaaagatttg ctcgaatggt tgcgacgtgc agtgtttctg catgtcgacc
      661 ctcaggcaaa ccaggtgctc gaattgcctg cagaacaggc gaaagacaca ctgcaactgg
      721 tacagcctct gccgcttgag tatctgatgt cagtgacctc tcacctggag cgggccatga
      781 cggcattgcg tcactccgtt catccaaggc tgttgtttga gttggagctg gtacgattgg
      841 ctcgacgcga agtttcgctc ggactcgagg gaaccgaacg gcgtttatcg caggtcgaac
      901 agcaactgcg cacaccgcga ctcgtcgcca gaccagtcgg tgctgcaccg actgtcgttg
      961 ggaccgccga accaggattg cgccgtgctg atgcggcagc gccgatgagg cctctgccag
     1021 tgaagaccga gacggcggtt cgatctcagc ctgcgcgccc ctacgaagcg cagcccaaac
     1081 cgaatctgac actggtaaaa cgcgaaccgg tggcgggttc tccggaacat ctttccccca
     1141 cagagcgatg ggcagcgttt cgccaggaga ttctgacgtg cagcacaagt actgggtcga
     1201 ttctggaaat ggcccgttat gaaggcttca aggatggcgt ggtgacgatc gtgtttggga
     1261 acgacttcag cgctcgccgt tttcgtgagc cgtcggttct ggaaagcatt cagccgattc
     1321 tcgaacagtt tttcggttcg ggaacgcgtt tggatgtccg tggggcgact tcgccctcac
     1381 cagtggctgt caaggagtcc gtgacgcagg tgaaagaaaa cattgcgaag atcgatcagt
     1441 cagtgaagga caagatcctg agccaatcaa cggttactga cactttagct gtttttggtg
     1501 gagaaattgt cggtattgaa aagaagtgat tatcagaata atgatgaaaa agaccgctga
     1561 gggcacaccg ttttccgagt tgatcccgtc gagtgaaccg ggcaagtaac ggtacagtca
     1621 gagccggcgc gcgtttggcg tcccctgttt tttgatcagg ttcgaatggt gcaaaaacat
     1681 gtggaaagtg tggaaaacct tggggataac tgaataaatc aaggtttttc acgtggaaaa
     1741 gaaaaaatct gtgccctctt gacaaacgct tccattgccg ctgtgggggg tacccccctg
     1801 ctgaagtcgg ccgttgaaag gttttttgtt tgtcaatagg gtagacaaaa aaaccggcag
     1861 aaagccaaac atggctttac tgccggtttt gccaaagaaa ctgattacac gaacagattg
     1921 atttccgcgt ccgaggctga tccgatatac gaggcaactc ctccgatctc gatgccatca
     1981 atgagttctt catgtttgat acccatgaca tccatgctca tcgagcaggc gaccattctc
     2041 acccctgcct gtctggcttt ggcaatcaga tcaggaagtg gctcgacctg tttctgagcc
     2101 atcacattct tcatcatcat ggtgcccatg ccggcaaaat gcatcttcga caggccgagt
     2161 gcttcaggac ctttgggcat catcatcccg aacatttgtt ccagagcggt tttaggcaca
     2221 gccggagctt cgcgtttacg cagaatgttc agaccccaga aggtgaagaa cagggtaact
     2281 ttcataccca tggcagcagc gccgttggca atgatgaaga cggcataggc acgatcgaga
     2341 tcggcactga ataccaccat ggtcaacgct ttctccgcct tcgtcatggg ctgagtgcga
     2401 caggcaacgg ctggagacag agcctctgcc gttttttcca tgagcaccgt gaaccggccc
     2461 ttgtctggaa atgccttcag cagcttgttt cctgaagact tggcccacga ggcggcatct
     2521 ggaggaaatc ccggatcggt cgccacgatt ttcagcacct gaccgggttt cagttcttcc
     2581 atgcgctcac gcatcttcag gagggggccc gggcactgca aaccgcagac atcgagttca
     2641 aaggcttctc cttcagcgat gggagacgtt gacgaagagc tgcagaagga ctctttcgtg
     2701 gagatttgag cgggaatg
//
LOCUS       DAORHD010000638         2718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44229_length_2718_cov_2.893729, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000638 DAORHD010000000
VERSION     DAORHD010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44229_length_2718_cov_2.893729"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..635)
                     /locus_tag="PKO06_13340"
     CDS             complement(282..635)
                     /locus_tag="PKO06_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV70678.1"
                     /translation="MTPPEATDFLLTIRHPADDTLEIGLSGALNEGTVAAFREALEQH
                     QAETFARLVFDCQRLSFLSSAGLSALVVVFQRRKRASQTMVLKHVQPMVRKILRLTRL
                     DQHLTIIDSDDPPAA"
     gene            794..1672
                     /locus_tag="PKO06_13345"
     CDS             794..1672
                     /locus_tag="PKO06_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70679.1"
                     /translation="MAVSGPTNQRKLETVGRLAAGITHEINTPMHYLGNNITFLKGAF
                     TDLLALQEQYRQILTDIRAGKTVSNHVWQTLAAQEQQADPDYLQQEITKALDQSIEGI
                     ERVTRIVLALKDFSHPSLHEYSLADLNQCVDTTVTICRHEWKRVADLDLHLEANLPLV
                     YCCRDDIHQALLNLIVNASHAVKARIDQGGYDRGRLTLTTTFDEARGQVLLSVADNGC
                     GIAPEHQARLFTPGFTTKPVGQGTGQGLLLMRQIIEERHHGAITWTTSVGKGTTFTLM
                     LPLRPDSDNDNTQAGS"
     gene            1678..>2718
                     /locus_tag="PKO06_13350"
     CDS             1678..>2718
                     /locus_tag="PKO06_13350"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV70680.1"
                     /translation="MGFFNDLYKRLTGADREAVTELKVLFADPEPNMLDGHRRLLRSQ
                     RPGWQGYYATSGTAALELLAVHNPVFLVTELGLKTPDGSELLSHTQRFFPETVRIVLS
                     SESDPEAESILRATQSAHQFLVKPCKPETLLTKIEQACLLRRLLRNPSLAKVIGGIPH
                     LPSLPPLYYELLAALDSENASLEDIGNIIARDVSMTTRVLHLVNSAFFGLPRRVAAPQ
                     EAAILLGVNIIKSLVLYVKLFFTAPESPIPGLSLDDLWAHSTLTASLAREITREMGGD
                     RRALEEAMLGGMLHDVGKLLVMDQGSYIRAVTRRMDEEKLPFWQAEYREFETSHAEIG
                     GYLLGLWGLPDPV"
BASE COUNT          587 a          830 c          775 g          526 t
ORIGIN      
        1 agaccgatct tcaggtagtt gatctgatcg gggttgtcga gcagaatcag cggcttgccg
       61 aatgccagat cgagttgcac ctgagactgg gcaccggcga tcagcgtcca ggtcagaaaa
      121 ccaacgacca ccaacagggt gcggaagaga gtatgcatag ttgcctccga aatttgaagt
      181 gggcgccgga acggcgtcgc tgcttcaaac ataggcggtg aggagtaacg ggtggtgaga
      241 tcgtggtaaa aggaatgtaa aaagagtgta aaacgtcacg atcacgcggc aggaggatcg
      301 tcggagtcga tgatggtcag atgctgatca aggcgcgtca aacgcagaat cttgcgtacc
      361 atcggctgca catgtttgag caccatcgtt tggctcgcgc gtttgcgtcg ctgaaagacc
      421 acgacgagag ccgatagtcc ggcgctcgac agaaagctca accgctgaca gtcgaacacc
      481 agacgcgcaa acgtctcagc ctgatgctgt tccagtgctt cacgaaaagc tgccacggtc
      541 ccctcgttga gcgctcccga gaggccgatc tcgagcgtat cgtctgccgg gtgtcgaatc
      601 gtcagaagaa aatcagtggc ttccggtgga gtcatacagt cattccattc atcgcctgaa
      661 taccgtaatt ctgcgtcatt gcccccttcg ttgtcaaacc acggttttcc cgatcaccgt
      721 cagacaatgc ttgtgttttg gcggcaaaac agggaaaatg cacaataatc cgcagcagca
      781 gaggatagac gaaatggccg tttccggacc cacgaaccag cgcaaactcg agaccgtcgg
      841 tcgcctcgcg gccgggatca cgcacgagat caatactccc atgcattatc tgggaaacaa
      901 catcactttt ttgaagggtg cgtttaccga tctgctggcc ctgcaggaac agtatcgcca
      961 gatcctgacg gacattcgcg ccggcaaaac ggtgagcaat cacgtctggc agacactcgc
     1021 cgcgcaggag cagcaggccg accccgacta tctgcagcag gaaatcacga aagcgctcga
     1081 tcaatccatc gaaggcatcg agcgggtcac ccgcatcgtg ctggctctca aggatttctc
     1141 acacccttca ctgcatgagt atagtctggc tgatctgaat cagtgcgtcg acaccacggt
     1201 caccatctgt cgacacgagt ggaagcgcgt cgccgacctc gatctgcatc tcgaggccaa
     1261 tctgccgctg gtgtactgct gtcgcgacga cattcatcag gcgctgctga atctcatcgt
     1321 caacgcttcg cacgcggtga aggcccgcat cgatcagggc ggttatgatc gcgggcgcct
     1381 gaccctgaca acgactttcg acgaggcgcg cgggcaggtt ctgctttccg tcgccgacaa
     1441 cggctgcggc atcgcccccg agcatcaggc tcgcctgttc accccggggt tcacgaccaa
     1501 gccggtcggc cagggaaccg ggcagggtct gctgctgatg cgtcagatca tcgaagagcg
     1561 tcatcacggc gcgatcacct ggacgacttc cgttggcaaa gggaccacct tcacgctcat
     1621 gcttcccctg cgccccgatt ctgacaacga caacacacaa gccgggagct gaaaaagatg
     1681 ggcttcttca acgacttgta caaacgactg accggcgcgg accgtgaagc ggtcactgaa
     1741 ctcaaggttc tgtttgccga cccggaaccc aacatgctcg acgggcaccg tcgcttgttg
     1801 cgttcgcagc gccccggctg gcagggttac tatgcaacgt ccgggacggc ggccctcgaa
     1861 ctgctcgcgg ttcacaatcc cgtgtttctg gtgacggaac tcggactgaa aaccccggac
     1921 ggcagcgaac tgctcagtca cacccaacgg ttctttcctg aaaccgtacg cattgtcctg
     1981 tcgagcgagt ccgatcctga ggcagagtcc attctgcgag cgacgcaatc cgcccatcag
     2041 tttctggtca aaccctgcaa accagaaacc ctgctgacca aaatcgaaca ggcgtgcctg
     2101 ctgcgccgac tgctgcgcaa cccatcgctg gcgaaggtga tcggcggtat cccgcatctg
     2161 ccgagcctgc cgccgctgta ctatgaactg ctggccgccc tcgacagcga gaatgcctcg
     2221 ctcgaagaca tcggcaacat cattgcccgc gacgtgtcga tgaccacgcg cgtgctgcat
     2281 ctcgtgaact ccgcgttttt cggattgccc cgccgcgtgg ccgcgccgca ggaagcggcg
     2341 attctgctcg gtgtcaatat catcaagtcg ctcgtgctgt atgtgaagct gttctttacg
     2401 gctcctgaaa gcccgattcc ggggttgtcg ctcgacgatc tctgggcgca cagcaccctg
     2461 accgcctcgc tcgcccgcga gatcacccgc gagatgggtg gcgaccgccg ggcgctcgaa
     2521 gaagccatgc tcggcggcat gctgcatgat gtcggcaagc tgctggtcat ggaccagggc
     2581 tcgtacatcc gcgccgtcac ccgacgcatg gacgaagaga aactgccgtt ctggcaggcc
     2641 gagtaccgcg agtttgaaac gtctcacgcc gaaatcggcg gctatctgct tggactctgg
     2701 ggtctgcctg acccggtc
//
LOCUS       DAORHD010000639         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44247_length_2717_cov_3.285124, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000639 DAORHD010000000
VERSION     DAORHD010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44247_length_2717_cov_3.285124"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKO06_13355"
     CDS             <1..327
                     /locus_tag="PKO06_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNV70681.1"
                     /translation="RYPLVFSRCAALSPSIWWENTWILSDLAARLESLKQTRFWVDIG
                     TREGDNPQAHLNNLRRVAELLRQAGLKEKTDFMAREIEGATHSEQAWSARFDQVLLFL
                     YPMRKR"
     gene            407..2560
                     /locus_tag="PKO06_13360"
     CDS             407..2560
                     /locus_tag="PKO06_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70682.1"
                     /translation="MIGRAKWLVVGLLVVGQLFGVNVEASGEPASATKIVKVKIVVEA
                     PNGARWAAEGLRVETLELGAFTTDERGSIELRVPRGYLGHIHVRRVVWQAMVGNKYVR
                     NWVTLHRLTQPRAGGASEKTRQFALQPMAFWNPRMVGVDPAFEHEEKELIFPVVHHRV
                     CLKDRDDKSTAGQVNVLPLENPKVGYVSLVFPPGASDPGAGPSEDPEGTQDLLLPLRE
                     GGQPPCGGLILSTVRTSGAREEMSSHVRVVPVPRQRQLLTARMGIEEIEMRQRVHKAI
                     GETERLTQAQMDRLRRVQVIFAATYGLVFRDAVGNPLFRPARYDHWLPGELYLPPDYS
                     FQTPDRCELYFHEWWHAVQDVCGIVSSGGAHSQPFSPCDDPLMALSEGMANLCGVVLG
                     QLLRTGDVGPEYAGKVLEHLNSCSATYRGQPRGVWEDGIFTAALLQHYTMTVGNDPAR
                     MLSRMIGDAIANSTVDRADTFFQNISAGTDADALNRKRQMSVLYRLIVPAPANAFAVA
                     IVDVVSGKPPMKAMRADTSVNLKAHLLFDGRPLLGSWTEVLELDWHTLEAGTKTLKAN
                     EPFTFRAPAPGTRRTISVTLRRRNPVDGPEILATSQIEIETGLEKAAELPETTAESRV
                     DAASGTGEWASPKISVAASDQWEVSRGRDEVSLTRRHLKRQRLFPDDAGVMKLADSAQ
                     AGGSISLRWLTPPVPRTAEAGCGHRTTSSLRCPAS"
BASE COUNT          551 a          765 c          861 g          540 t
ORIGIN      
        1 cggtatccgc tggtgttttc acgttgtgcc gcgctctcgc cttcgatctg gtgggagaac
       61 acctggattc tgagcgatct ggctgctcgt ctcgaaagcc tgaaacagac tcgtttctgg
      121 gtcgacatcg gtacccgcga aggcgacaac ccgcaggcgc atctgaataa cctgcgacgt
      181 gtggctgagc ttctgcggca ggcaggtttg aaagagaaaa ccgatttcat ggcgcgcgaa
      241 attgaagggg ccacccatag tgagcaggcc tggtccgcac gcttcgacca agttcttctg
      301 tttctctacc ccatgcgcaa gcgctgaatg agaaagtaac aaaagaggag cctggacaat
      361 acaggctcct tttttcataa ggagagaatt cgtgatgagg tggtcgatga ttggcagagc
      421 gaagtggttg gttgtcggtc tcctggtggt gggccagttg tttggcgtga acgtcgaagc
      481 ttctggcgag cccgcatccg cgaccaaaat cgtgaaggtg aagatcgttg tcgaagcgcc
      541 gaatggggcg cgctgggccg cggaaggact tcgtgtcgag acactggaac tcggagcttt
      601 cacgacggac gaacgaggct cgatcgaact tcgcgtacct cgcgggtacc tcggtcacat
      661 tcacgtgcga cgcgttgttt ggcaggccat ggtggggaat aaatacgtac gcaactgggt
      721 caccttgcac cgcctgaccc agcctcgtgc aggcggagcg tccgagaaga ccaggcagtt
      781 cgctctgcaa ccgatggcgt tctggaatcc caggatggtc ggtgtcgacc ctgcgttcga
      841 acacgaagag aaagaactta ttttccccgt ggtgcatcac cgcgtgtgtt tgaaagaccg
      901 cgacgacaag tcgacggcgg gccaggtgaa cgtgcttccg ctcgagaacc cgaaggtcgg
      961 gtatgtttcg ctggtttttc caccgggagc ttcggacccg ggagcaggac catctgaaga
     1021 ccccgaggga acgcaggatc tgcttttgcc gttgcgcgag gggggacaac caccatgcgg
     1081 cggcctgatt ctatcgacgg tgcggacctc tggcgcgcgc gaagagatgt cgagtcatgt
     1141 gcgagttgta ccggttccgc gacaacggca gctgctcact gcgcgtatgg gaatcgagga
     1201 aatcgagatg cgccaacgag tgcataaggc gatcggcgag acggagcgtc tgacgcaggc
     1261 acagatggac agactgaggc gcgtacaggt gatcttcgcc gccacctacg gtctcgtgtt
     1321 cagggatgcg gtggggaatc cgttgtttcg cccggctcga tatgatcact ggctcccggg
     1381 tgagctgtat ctcccgcctg actatagctt ccagactcct gatcgctgtg aactgtactt
     1441 tcacgaatgg tggcatgccg ttcaggatgt ctgcgggatc gtcagttcgg gcggcgcgca
     1501 cagtcagccg ttctcgccct gtgacgaccc actgatggcc ttgtcagaag gaatggccaa
     1561 tctctgcggc gtcgtgttgg gtcagctgct tcgtaccggt gatgtcggcc cggaatacgc
     1621 ggggaaagtg cttgaacatc tcaattcgtg ctcggcaacg tatcggggac aaccgagagg
     1681 cgtatgggag gacggaatct tcacagcggc tcttctgcaa cactacacga tgacggtggg
     1741 gaatgacccg gcgcggatgc tctcgcgaat gatcggcgat gccattgcca acagcacggt
     1801 cgaccgagcg gacacctttt ttcagaacat ctcggccggc accgacgccg acgcgctgaa
     1861 tcgcaaacga cagatgagcg ttctctaccg gttgatcgta ccggcgccgg ccaacgcgtt
     1921 tgcggttgcc atcgttgatg tcgtctctgg aaagccgccg atgaaagcga tgcgagccga
     1981 tacgagcgtg aatctgaaag cgcacctgtt gtttgacggg cgaccattgc tgggctcctg
     2041 gaccgaagtg ctcgagctcg actggcacac gctcgaagcc gggacaaaga ccctcaaagc
     2101 gaatgagccg ttcacgttcc gtgcgccggc tccgggaacg cgccgcacga tatcggtaac
     2161 cctgcgtcga cgcaacccgg tcgacggacc cgagatcctc gccacgagcc agattgaaat
     2221 cgaaactggc ctggagaaag cggcggagct tccggagacg actgcagaat ctcgcgtcga
     2281 tgccgcttca ggcactggtg agtgggcttc tccgaagatc agcgtcgcgg cctccgacca
     2341 gtgggaagtc tcgcgcggtc gtgatgaagt gtcgttgacc cgtcgccatc tcaagcgtca
     2401 gagactgttt cccgatgatg ccggggtgat gaagcttgcc gattcggcgc aggccggtgg
     2461 ttcgatcagc ctgcgctggc tgacgccccc tgtgccgcga actgcggaag cgggctgcgg
     2521 acatcgaacg acaagttcct tacgctgccc ggcttcgtag cccggcagcg tggatgaccg
     2581 cccaagaaat gcggctgcga gcagaccaga tcgacgaggt gatactgccg tcgatcatgc
     2641 ccacgtacga ggcgtactac tcgatgccgc gcctaccgac acaaccgaaa tcgtcccgtc
     2701 ccaactttcg gcgggtg
//
LOCUS       DAORHD010000640         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44251_length_2717_cov_3.080391, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000640 DAORHD010000000
VERSION     DAORHD010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44251_length_2717_cov_3.080391"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PKO06_13365"
     CDS             <1..344
                     /locus_tag="PKO06_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNV70683.1"
                     /translation="GKLKVGDLLTVRIELRTDRDMEYVHMKDGRGSGLEPTETLSQYR
                     MQDGLYYYHATRDTASHFYIDYLPKGTYVFEYTLRVQHKGRYQTGMAEIQCMYAPEFN
                     SHSESFVLEVE"
     gene            485..1579
                     /locus_tag="PKO06_13370"
     CDS             485..1579
                     /locus_tag="PKO06_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70684.1"
                     /translation="MRWLNSWAERPIPRRCRPLCLVVLLLLTGGWVFGQTELSPSTSP
                     TSLAEASAASINLPDSRPAQLVASGPEEVMTGFYLISGGKLDFQNSTVPIDFYFWMTY
                     PGTETPKIEFMNSRNFILTPQTTQTIEGPNGRFQNICYRVTGTFDQDLDMKKYPFDRF
                     KIRILAEDTEKTIQERVFVPDTGSTGIDASFKILGWNVTDRDIRTTTHAYASTFGIQN
                     TEGGQERYSQIIFEMDISRDHAIIFIKIVSPVLLFLSIAALGLFLNVDQISQKISLSV
                     AALFSSVAYHINVTQGLPPVGYLTFIDRMMLGQYLVIFMNLLFCVGLFWTNKNEHKRL
                     ERILLITSRILVPLAALCVLWVLFSQVEFS"
     gene            1603..2448
                     /locus_tag="PKO06_13375"
     CDS             1603..2448
                     /locus_tag="PKO06_13375"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNV70685.1"
                     /translation="MSHNSSSETSVTGTGKFSFVLFWSVAAGLPLLSFLLLPQMGDAG
                     MNLWLPVVTAAWALFSLASLLFPSRFAQLGRASLYGFAGTGALYLLLTAFPVGEWISR
                     PLLLAPADGNADAIMVLAAGIGEDAQPGFATYQRTLHGLNLYRQKRAPRLIFSTSNQI
                     DARSGHHESEWALRLTALFGLATDSYFIMTEGGTTRGEAAGAARLYQPFGIRRLLLVT
                     NGPHIRRATRVFEKAGFEILPAPVQTENSIRTYAETGVSRFTQALHEWIGLFVYRIRG
                     DIAAF"
     gene            2488..>2717
                     /locus_tag="PKO06_13380"
     CDS             2488..>2717
                     /locus_tag="PKO06_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70686.1"
                     /translation="MKTRCAMCVQVCALCLVYLLLMGVHQGSAHYRFPVSDDLIEIEI
                     IGDDSGRFPLYPAPYGDSRESDQRWYLAARRG"
BASE COUNT          519 a          827 c          734 g          637 t
ORIGIN      
        1 ccgggaaact caaggtcgga gatctactca ccgtgcgcat tgagctgcga actgaccggg
       61 acatggagta tgtccacatg aaggatggcc ggggcagtgg cctggaaccg acggaaaccc
      121 tttcacagta tcgcatgcag gatggtctct actattatca cgccaccagg gacacggctt
      181 cgcacttcta catcgactac ctgccgaagg gcacgtatgt gttcgagtat acgctgcgag
      241 tgcagcacaa aggccgctat cagacaggca tggccgagat tcagtgcatg tacgcgcctg
      301 aattcaacag tcattccgag tcgttcgtgc ttgaggtcga gtaaacacct cgtctcgttc
      361 tgaccacggg ccgcctgctg ccctcacgca ggcggcccct tctttttccc tcacggacga
      421 gttgaaccgg gttggggaat tccgtatagt gtctcccatg gttgtgaatc acttgtggca
      481 cttcttgcgc tggttgaatt cgtgggctga aaggcccatt ccccgtcgtt gccgccccct
      541 gtgcctggtg gtcctgctgc tgttgaccgg cgggtgggtc ttcggccaaa cagagctttc
      601 tccctcgacc tcgccgacgt ccctggccga agcttcggcc gcgtccatca accttcccga
      661 ttcccgcccc gcgcaactgg ttgccagcgg gcccgaagaa gtcatgaccg gtttttatct
      721 catttcaggc ggcaaactcg acttccagaa ttccacggtt ccgattgatt tctatttctg
      781 gatgacctac cccgggaccg agaccccgaa aatcgagttc atgaacagtc gcaatttcat
      841 tctgacgccg cagacaacgc aaaccatcga aggccccaat ggccgattcc agaacatctg
      901 ttatcgcgtc accggcacct tcgaccaaga cctcgacatg aaaaagtacc cgtttgatcg
      961 attcaagatt cgcattctgg ctgaagatac agagaagaca attcaagaac gagtcttcgt
     1021 gcccgacacc gggtcgaccg gcatcgacgc cagtttcaag attctgggct ggaacgtcac
     1081 cgaccgcgat atccgcacga cgactcacgc gtatgcctcg accttcggta tacagaacac
     1141 cgaaggaggg caggaacgat actctcagat catattcgag atggatatct cgcgagacca
     1201 cgcgatcatc ttcatcaaga tcgtgtcgcc cgtgctgctg tttctctcaa tcgccgcttt
     1261 ggggctcttc ctgaatgtcg accagatcag tcagaagatc agcctctcgg tcgccgcact
     1321 gttcagttcg gtggcgtatc acatcaacgt cacacagggt ctgccccccg tcggttatct
     1381 caccttcatt gaccgcatga tgctcggaca gtatctggtc attttcatga atctcctctt
     1441 ctgcgtcggc ctgttctgga ccaacaagaa tgaacacaaa cgcctcgagc ggatcctgct
     1501 gatcacctca cgcattctgg ttcctcttgc ggctctgtgc gtgctctggg tattgttctc
     1561 gcaggtcgag ttttcctgat catcacgggc tgtggctagg tcatgagcca taattcatca
     1621 tctgaaacat cggtaaccgg taccggaaag ttttcgttcg tgctgttctg gtctgttgcc
     1681 gcgggccttc cgctgctcag ttttcttctg ctgccgcaga tgggcgacgc cggcatgaat
     1741 ttgtggcttc ctgtcgtgac cgcggcctgg gcgttgttct ccctggcgtc gcttctgttc
     1801 ccctcgcgat tcgcccaact cgggcgggcc agtctgtatg gctttgccgg caccggcgca
     1861 ctgtatctgt tgctgactgc ctttccggtc ggcgagtgga tctcgcgccc cctgctgttg
     1921 gcaccggcag acggaaacgc cgacgccatc atggtgctgg cggcgggcat cggcgaggat
     1981 gcccaaccag gcttcgcgac ctaccagcgc acacttcacg gcctcaatct gtatcgacag
     2041 aagcgcgccc cgcgcctcat cttcagtacc agtaaccaga tcgacgcgcg gtccggacac
     2101 cacgaatcag aatgggcgtt gcggttgacc gctctattcg ggctggccac tgattcgtat
     2161 ttcatcatga ccgaaggcgg cactacccgg ggagaggcgg cgggagccgc ccgcctgtat
     2221 cagcctttcg gcattcgccg tctgctgctg gtaaccaacg gcccgcacat tcgtcgcgcg
     2281 acccgggtgt tcgaaaaagc cggcttcgag attcttcccg ctcccgtgca gaccgaaaac
     2341 tccattcgca cctacgccga aaccggagtg tctcgattca ctcaggcact gcatgaatgg
     2401 atcggtcttt tcgtgtatcg aatccgcgga gacattgctg cgttttgaga gtcaggggcc
     2461 gaaaaaccgc ccccgggagg tgttgtcatg aaaacccgtt gtgcgatgtg cgtgcaggtt
     2521 tgtgcgttgt gtctcgtcta tctgctgttg atgggtgtcc accagggttc cgcccattat
     2581 cgttttccgg tttccgatga tctcatcgag atcgaaatca tcggcgacga ctccgggcga
     2641 tttccgctgt atcccgcccc gtacggagat agtcgcgaga gcgatcagcg ctggtatctc
     2701 gccgctcgac gcgggga
//
LOCUS       DAORHD010000641         2714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44321_length_2714_cov_3.581798, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000641 DAORHD010000000
VERSION     DAORHD010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44321_length_2714_cov_3.581798"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKO06_13385"
     CDS             complement(<1..1075)
                     /locus_tag="PKO06_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70687.1"
                     /translation="MKSFLHGKRGVALPFTILAMIVISGFVITMSSLNQGLKNQIFHT
                     NNHQLSFLLAYSAFSKVLAKIHSNSWTDRPFRDGPYAENNVAFQGGTYDLFVENSPGK
                     QYQADIYVRTSLAGITRLYFWRIRFNDDLLDISSRITVEFFTNGEPGDFPKTAGPKSF
                     SSKVDGLLARRASNQDKSDRLARDISDTKGTKAILEKMDGRPLKPFSDSYPIDPEDQQ
                     MNTKDPAAFPKMKPIAKGELPTADGPGTTPPAAGGFPPLTPVSSALDTTSLEQLAKAI
                     ASNSQGVASYVDKGWDEIDTKGTAGIASAQQNYVQANQMKDQAYSDMNSLISQANSML
                     GEAPSAEAKAAIEEMVSQAVTAGV"
     gene            complement(1202..>2714)
                     /locus_tag="PKO06_13390"
     CDS             complement(1202..>2714)
                     /locus_tag="PKO06_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70688.1"
                     /translation="FSAAIDAFRKALQKRQNPQWLYAVATLHDNLGEATEAMKAFEEL
                     TKQTLPKPVMVRVQLGLARNADFLGETTAAIEAYKKVIKEDPSLEDPFIGLLKLMRRT
                     GFSKGVDKIREKGLPYHGDKFTFHFWLGNLYYEIGDDKNAMEAFQKCIELKPENSSPY
                     YFIYKIQRKSQKIEEAISFLEKFYLNNPLLPYMFFEAALDARTENRLDLAFKFLRASI
                     LQDRLLLGRDDRGTIVAVERYVEKNGSAEEKLFLKPFMMFVNSEFRPASTEARRVLPK
                     LKDKLLRDDAERIIRHATQFVAGEDAYNEYVSHMQSEQEAAMAALKQRMAQGKVAGSP
                     DDKFGEIKRRALANPRDAKLQYGTALELARGGDLDGAKLFLRETLRANPFVSEAYYSL
                     AKIAEVEGDQAEIMRALQQALKINPNNSQARSMLAASYLAAGDFEQAVGEARSALLAN
                     PNNGEARLILARINIQNQRSEEALNEIELGLMVERDPVRKDQLLALKQQVGSR"
BASE COUNT          521 a          711 c          810 g          672 t
ORIGIN      
        1 ctacccccgc cgtgacggcc tgagacacca tttcttcgat ggcagccttg gcctcagcgc
       61 ttggggcctc tcccagcatc gagttcgcct gactgatcag ggagttcata tcgctgtagg
      121 cttgatcctt catctggttt gcctgcacat agttctgctg ggcgctggca ataccggctg
      181 tgcctttggt atcgatttcg tcccagcctt tgtcgacgta gctcgcgacg ccctgggaat
      241 tcgaggcgat ggctttcgcg agttgctcga gcgaagtggt atcgagagcc gaggacaccg
      301 gggtcagcgg agggaagccg ccggcggccg ggggagtcgt tcccggaccg tctgccgtgg
      361 gcagttcccc tttcgcgatc ggcttcattt tgggaaaggc cgcgggatct ttggtgttca
      421 tctgctgatc ttcggggtcg atcgggtacg agtcggagaa tggtttcagc ggccgtccat
      481 ccatcttttc gagaatggct ttcgttccct tggtgtcgga aatgtcgcgg gcaagacggt
      541 ctgatttgtc ctgattcgag gcgcggcgtg ccagcagacc gtcgaccttc gacgagaagc
      601 tcttaggtcc ggcggttttg ggaaagtccc ccggctcacc attggtgaag aactcgacgg
      661 taatgcggct cgaaatgtcg agcagatcgt cgttgaagcg aatacgccag aaatacaggc
      721 gcgtgatacc ggcgagactc gtacgaacgt aaatgtcggc ctgatactgc ttccccggag
      781 agttttcgac gaagagatca taggttccgc cctggaatgc cacgttgttc tcggcatacg
      841 gaccgtctct gaatggtcga tcggtccagg aattggaatg aatcttcgcc agaactttcg
      901 aaaaagccga ataggccagc aggaatgaca gctgatggtt gttggtgtga aagatctggt
      961 ttttcagacc ctggttcagg ctcgacatgg tgatcacgaa ccctgagatc acgatcattg
     1021 ccagaatcgt aaacggcaga gcgacgcctc tcttcccgtg cagaaagctt ttcatatgat
     1081 tctccctgtc tctcatactt gtcgaagggc ccgaactatt tcatcacaat ttttctgatt
     1141 cccaagatcg cggattcgca ggtctcgtcg tgtccgtcag tttccggaac tccggctgtc
     1201 tctagcggct gccgacctgt tgcttcagag ccaacaactg gtctttgcgc acggggtcac
     1261 gttcgaccat cagtcccagc tcgatctcgt tgagcgcctc ttcggaacgc tgattctgga
     1321 tgttgatacg ggcgagaatc agccgggctt cgccattatt cggattagcg aggagcgcac
     1381 tgcgagcctc accgactgct tgttcgaaat ctccggcggc gagatacgac gcggccagca
     1441 tcgatcgagc ctggctgttg ttcggattga tcttgagggc ttgttgcagg gcccgcatga
     1501 tttctgcctg atcgccctcg acctcggcaa tttttgccag cgaatagtac gcttctgaaa
     1561 cgaaggggtt ggcccgcagg gtctcgcgca gaaagagctt ggctccgtcg agatcgccgc
     1621 ctcgagccag ttcgagagcc gttccatact gcagcttggc atcgcggggg ttcgccagcg
     1681 cgcgtcgttt gatttcaccg aatttgtcat cgggactgcc ggcgaccttg ccttgagcca
     1741 tgcgctgttt cagggcagcc atggccgctt cctgttcgga ctgcatgtgt gacacatatt
     1801 cgttgtatgc atcttcaccg gcaacgaact gagtcgcatg acggataatg cgctcggcat
     1861 catcgcgcag cagcttatct ttgagtttgg gcagcacgcg gcgggcttcg gtgctggccg
     1921 ggcggaactc gctgttgacg aacatcatga atggtttgag gaagagtttc tcttcggcgg
     1981 aaccgttctt ttcgacgtac cgttcgacgg cgacgatggt gccgcgatcg tcgcgaccga
     2041 ggagaagacg atcctggaga atggaggcgc gcagaaactt gaaggcgaga tcgagtcggt
     2101 tttccgtgcg ggcatcgagt gccgcctcga agaacatgta cggcagcagc gggttgttca
     2161 gatagaattt ttccagaaag gagatcgctt cctcaatttt ctgggatttt cgctggatct
     2221 tgtagatgaa ataatacgga gaagagttct caggcttgag ttcgatgcat ttctgaaagg
     2281 cttccatcgc gttcttgtcg tcgccgatct cgtaatacag attcccgagc cagaaatgaa
     2341 acgtgaattt gtcgccatgg tagggcaggc ctttttcgcg aatcttgtcg acacctttgc
     2401 tgaagccggt gcgccgcatc agtttgagca agccgatgaa tgggtcttcg agagacgggt
     2461 cttccttgat gaccttcttg taagcctcga tggcggcagt tgtttcgccg agaaagtcgg
     2521 cgttgcgggc cagaccgagt tgaacacgaa ccatgacggg tttgggaagc gtctgtttcg
     2581 tcagttcttc gaacgctttc atggcttcgg ttgcttcacc gagattgtcg tgcagggtgg
     2641 caacggcata gagccactgc gggttctgtc gcttctgcag cgctttgcga aaggcatcga
     2701 tcgcggcgga aaag
//
LOCUS       DAORHD010000642         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44409_length_2710_cov_4.558569, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000642 DAORHD010000000
VERSION     DAORHD010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44409_length_2710_cov_4.558569"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2041)
                     /locus_tag="PKO06_13395"
     CDS             complement(<1..2041)
                     /locus_tag="PKO06_13395"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HNV70689.1"
                     /translation="MNRALQESLVSERLIRVYFCSPFASLQDERTHLLAHLYPRLEKF
                     CLDRGVSFVPVDVRLDRHANVAPSPDLLPVLFDDLVRSRPFFIGIFSGENDPPFPYLP
                     PDLLARFPKLAEMSGASLSDLEVELAVLNRPQSHEEAYFYFLGARDKPHRPPSWTDRL
                     KATTTPIRENIRSADELAELIFTDLRTAIERLFPEPAPLSVQRRRQETQESFARRHAL
                     SHLWRQTTFDKLSQHVQLDEGPPLVVVGPTGSGKSGLLAAWALRHRSAFPDDFCHLHF
                     VGSSPEDTQLDYILRRTIADMKAQLGLPEHIPHAPRDLKAEFALWLHLASARSRTIII
                     FDGIDKLQSSATTIDLSWLPIALPARVRIMLSVSPSSGKPLGIPESWPLLMLDPLNAA
                     ERRQFIEEYLGPYKAKISQPRLQRLIEAPGSGNPLILRSLLWEMRRKNFAESKDDQFR
                     ELLLRGSVEELTRELMTRAHRDLDLERPELVSETLSLLWAARRGLTDRELQDLLAAPD
                     HPLPHLQMSVVRSRLDGLVFSHNGLMSLFHPSLRELIEMRFLKDPDKCRELHLKLAEY
                     FLKSPTAWRRLEEGPWHLYKAGATKELRSWLFQPEIFAEAWGSNPDDVRLFAHVCFQE
                     PGETLVQLFDELIKHPAQFSPQAVAGAAEFLFERGHVPEAISLFRGLVRLFRQSGA"
     gene            complement(2038..>2710)
                     /locus_tag="PKO06_13400"
     CDS             complement(2038..>2710)
                     /locus_tag="PKO06_13400"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HNV70690.1"
                     /translation="YLDMGYNRERIGVLVWAGDDLDNAYYMPWANAIYLGDGGERFFP
                     LSREESIIYHEYAHAVVDKIQPLRGTEGSAMNEGQADYFACSFSNEPRVGEYVVSKKN
                     RPYLRILEHALHYPEDLQHEPHDDGRIWAGTLWDIRKQLGADVTDRLILRSFFLLKEE
                     PGYLDAILALLEADRQLNAGEHLTVIRDCFQARGYPLDAPNLFSARELKHLIRFPSLQ
                     QEDRP"
BASE COUNT          587 a          746 c          849 g          528 t
ORIGIN      
        1 tggcgcctga ctggcgaaac aaacgtacca ggccgcggaa cagggaaatg gcttcgggaa
       61 cgtgtccgcg ctcgaacaaa aactcggctg cgcccgcgac agcctgcggt gaaaactgcg
      121 ccgggtgctt gatcagctca tcgaacaatt gtaccagtgt ttctccgggc tcctgaaaac
      181 agacatgggc gaacaaccgc acgtcgtccg ggttcgagcc ccaggcttca gcaaagatct
      241 cgggctgaaa cagccatgaa cgcagctctt tcgtcgcacc tgccttgtac agatgccagg
      301 gaccttcttc gagccggcgc caggcggtgg gcgacttcag aaagtactcg gccagcttca
      361 gatgcagctc ccggcacttg tccggatctt tcagaaagcg catttcgatc agttcacgca
      421 gcgaggggtg aaacagactc atcagcccgt tgtgtgaaaa gacgagcccg tcgagccgcg
      481 accgaacgac gctcatctgc agatgcggca gagggtgatc cggggctgca agcagatcct
      541 gcagttcgcg gtcggtcaga ccacggcggg ccgcccagag cagcgagagt gtttccgata
      601 ccagctcggg gcgctcgaga tcgagatctc gatgtgcccg agtcatcaat tcacgcgtga
      661 gttcctcaac cgacccgcgc agcagcagtt cgcggaactg atcatccttg ctctcggcaa
      721 aattcttgcg tcgcatttcc cagagcaggc tgcgcagaat caacgggttt cctgatccgg
      781 gggcttcaat caatctttgc aggcgaggtt gtgagatctt tgccttgtac ggtccgagat
      841 actcctcgat gaattgacgc cgttcggcgg cgttcagcgg atcgagcatc agcaacggcc
      901 acgattcggg aattcccagc ggcttgcccg agctcggcga gaccgacagc atgatccgca
      961 cacgcgccgg cagcgcaatg ggcagccatg acagatcgat ggtcgtggcc gagctctgaa
     1021 gcttgtcgat accgtcgaag atgatgatgg tgcgcgatcg ggccgaagcc aggtgcaacc
     1081 acagcgcaaa ctcggccttc agatcgcgcg gcgcatgcgg aatgtgctca ggcagtccga
     1141 gctgagcttt catatcggca atcgtgcggc gaagaatgta atcgagttgc gtgtcttctg
     1201 gcgaactgcc gacgaaatgc agatggcaga agtcgtcggg aaacgccgaa cgatggcgga
     1261 gtgcccaggc cgcgagcaga cctgattttc cactgccggt cgggccgacg acgacgaggg
     1321 gtggaccttc atcgagctga acatgctgac tcagtttgtc aaaggttgtc tgtcgccaga
     1381 gatgtgagag cgcgtgtcgg cgggcgaatg actcctgggt ctcctgacgc cggcgttgga
     1441 ccgacagagg cgcaggctcg ggaaacaggc gctcgatggc tgtgcgcaga tcggtaaaaa
     1501 tcagttcggc cagttcatcg gcactgcgga tgttttcgcg aatcggtgtc gtcgtcgctt
     1561 tcaggcggtc ggtccaggat ggcggccggt gtggtttgtc ccgggccccg aggaagtaga
     1621 aatacgcttc ttcatgtgac tgtggccgat tcaggacggc aagctcgact tcgagatccg
     1681 agagtgaggc accggacatc tcggcaagtt tgggaaacct ggcgagcaga tcgggcggca
     1741 gataggggaa cggcggatcg ttttctccac tgaagatgcc aataaaaaag ggtcggctgc
     1801 gcacaaggtc atcgaacaac accggcagca ggtcggggct gggtgcgacg ttcgcatgtc
     1861 gatcgaggcg aacatcgact gggacaaacg acacgccgcg atcgaggcaa aacttctcga
     1921 gacgcggata cagatgtgcg agcagatgcg tgcgctcgtc ctgcagcgag gcaaacgggc
     1981 tgcagaaata cacgcgaatc agtcgctcgc tgaccagtga ttcctgtagc gcgcggttca
     2041 tggacggtct tcctgttgga gagatggaaa tcggatcagg tgcttcagtt cacgagcaga
     2101 aaagagattg ggtgcgtcga gtggataccc gcgtgcctga aaacagtcac gaatgaccgt
     2161 gagatgttcg ccggcattga gctgccgatc agcttcgagc agcgccagaa tggcatccag
     2221 gtagccgggt tcctctttca gcagaaagaa gctgcggagt atcagtcgat cggttacatc
     2281 ggctccgagt tgtttgcgga tgtcccagag ggtgccggcc cagatgcgcc cgtcatcatg
     2341 tggctcgtgc tgcagatctt cagggtaatg cagcgcgtgt tcgagaattc tgagataggg
     2401 ccggttcttc ttgctgacga catactcgcc gacacgcggc tcgttcgaga acgaacaggc
     2461 gaaatagtcg gcctgacctt cgttcatggc gcttccttca gtgccgcgca gaggctgaat
     2521 cttgtcgacg acggcatgag cgtactcatg gtagatgatg ctctcttcgc gcgacaacgg
     2581 aaagaatcgt tcgccgccat cgccgaggta aatcgcgttg gcccagggca tatagtaggc
     2641 attgtccagg tcgtcgccag cccagaccag caccccaatt cgctctcgat tataacccat
     2701 gtcgaggtaa
//
LOCUS       DAORHD010000643         2710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44432_length_2710_cov_2.677213, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000643 DAORHD010000000
VERSION     DAORHD010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44432_length_2710_cov_2.677213"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PKO06_13405"
     CDS             complement(<1..186)
                     /locus_tag="PKO06_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70691.1"
                     /translation="MNPVLVPWVAAIVVPLGLFAWYFRKSWTDEVLVTSTLIGLIVGA
                     IAVLPIKYAVYPGIEWVL"
     gene            complement(183..1640)
                     /locus_tag="PKO06_13410"
     CDS             complement(183..1640)
                     /locus_tag="PKO06_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNV70692.1"
                     /translation="MYCSRCGQQLVSRLSKCPACDTPHKLKHRRRRRLLLGLFLFLSG
                     AFVGSLVDGWYFQGGSWGHSMIMPWLKRAPTPTASSTDPANNVPTVHSGNEPLPTVSA
                     VAPPVSFRPPSNKPATGHKTNEPAKPVVVESAKPVASAADKAAAASDSVPAAASAVVV
                     VASSAAVVPPAPAEPASAVSTAATPAIATAASVVVADKPYDLEYKGVETLEEGDSTNY
                     HGSLSGDAPYLLFSSNRQQREGKSVYQSFVRDLSGKAGSVRVFDWPGNVWTPEFARDG
                     AVLVFTSDSQEKEHVFLYDRKTQDFRQLTQGKSKNQMAAMSPDGRFVAFTSDRAGNND
                     IWMIGSDGQGLIQVTRGAEDEREPRWSPDGRSLYYTKIVERLKTSHIMKIELDPLGQP
                     VEVVGGTVRNWLPDVSPDGAYLAYVQSQNSDGSGNVLRLRRLADGQDQVIKPFPGSEH
                     FRPIWSSDGHKLVFHAERKGRKSLFAAQLQRKPIP"
     gene            1772..>2710
                     /locus_tag="PKO06_13415"
     CDS             1772..>2710
                     /locus_tag="PKO06_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HNV70693.1"
                     /translation="MNLKKRIGQLMVVGFQGLEPSDDFLKFVEEWGIGGFIFFARNID
                     RAERIPQVVRRLEDAAGGPIFTSIDQEGGLVMRILTSGSPFPGAMALSATDDVDLTRK
                     IHTAIGREMRALNLNWNLAPVLDINHRDNPGIGARSFGEDPERVSRFGCAAVEGLQAG
                     GVLACAKHFPGKGHARVDSHLTLPTIPYDTARLESFELQPFRAAIRSGVASIMTSHVF
                     FPAYESTPNLPATLSKAVLTDLLRGKLGFTGLLVTDDLEMGAITEAYGVAEAGFRSFM
                     AGADLLLICHDLDRERSCAEKILDECRHNPDVEKRIN"
BASE COUNT          569 a          803 c          784 g          554 t
ORIGIN      
        1 cagtacccat tcgatcccgg ggtagacggc gtatttgatc ggcaacacgg cgatcgcgcc
       61 gacaatcagg ccaatcagcg ttgaagtgac cagcacttcg tcggtccagg atttgcgaaa
      121 gtaccaggcg aagagcccca gaggcaccac gatcgccgca acccagggaa ccaacacagg
      181 attcaaggaa taggcttcct ctgcaactgg gcggcaaaca ggctcttgcg ccccttgcgt
      241 tcggcatgga acaccagctt gtgaccgtca ctcgaccaga tagggcgaaa gtgttctgag
      301 ccggggaatg gcttgataac ctgatcctga ccatccgcga gacgacgcag gcgcagcacg
      361 ttcccgctgc cgtcggaatt ctgcgactga acataggcga gatacgcgcc atccggcgaa
      421 acgtctggta accagttgcg aaccgtgccg cccaccactt cgaccggttg gcccagggga
      481 tcaagttcga tcttcatgat atgcgaggtc ttcagccgct cgacgatttt cgtgtagtac
      541 aggctccgtc cgtcagggct ccaacgcggt tctcgttcat cttcggcccc gcgcgtcacc
      601 tgaatcaacc cctggccatc cgagccgatc atccagatgt cgttgtttcc ggcacgatca
      661 gaggtgaaag ccacaaaccg tccgtcaggc gacatcgccg ccatctggtt tttcgacttt
      721 ccctgggtca actggcgaaa gtcctgagtt ttgcggtcgt agagaaaaac gtgctctttt
      781 tcctgactgt cgctggtgaa caccagaacc gccccgtcgc gtgcaaattc aggcgtccag
      841 acgtttccgg gccaatcaaa gacgcgcacc gagccagctt ttccagaaag gtcgcgtacg
      901 aagctctgat acaccgattt tccttcgcgc tgctgacggt tcgaggaaaa cagcagatac
      961 ggtgcgtcgc ccgagaggct gccgtgataa ttggtgctat cgccttcttc gagcgtttcg
     1021 acgcccttgt attcgagatc atagggtttg tccgccacca ccactgacgc tgcggtcgct
     1081 atggcgggtg ttgcggcagt cgaaacagca gacgcgggct cagcaggtgc cggggggacc
     1141 acagccgctg acgaagccac gacgaccacc gcgctcgcag cggcgggcac ggagtctgac
     1201 gccgcggcag ccttgtctgc agcactggca accggtttcg ccgattcgac aacaaccggt
     1261 ttcgcaggct cattcgtctt atgcccggtc gcaggcttgt tgctcggcgg acggaacgac
     1321 actggcgggg ccacggcaga aaccgtgggt aacggctcat tgcctgaatg caccgtcgga
     1381 acgttgttcg ccggatctgt ggacgaggcg gtcggtgtag gtgccctttt caaccagggc
     1441 atgatcatcg agtgccccca gctgccaccc tgaaaatacc agccgtcaac cagactcccg
     1501 acgaaagctc cggagaggaa gagaaacagg ccgagcagca gccgtcgccg ccggcgatgt
     1561 ttgagtttgt gcggtgtatc gcatgcaggg catttgctca gtcgggaaac cagttgctgc
     1621 ccacagcggg aacaatacat acctgaccct cctttacacg tggagacgtg tcattataaa
     1681 gtgaccccgt cgaaaaagaa agggcacccg gcaccacgat aagaccgcaa gtgctcaata
     1741 aaccgcccgg ggtacaggaa ggactgcgca gatgaatctc aaaaaacgta ttggtcagct
     1801 catggtcgtc ggttttcagg gtctcgaacc cagcgatgac tttctgaaat tcgtcgagga
     1861 gtggggcatc ggaggattca tcttctttgc gcgtaacatc gaccgtgccg aacgcattcc
     1921 gcaggtcgtg cgccgactcg aagacgcagc cggtgggccg atcttcactt cgatcgatca
     1981 ggaagggggc ttggtgatgc gcattctcac cagtggcagc ccttttcccg gagcaatggc
     2041 cctttcagcg accgatgatg tcgatttgac gcgaaagatc cataccgcca taggtcgtga
     2101 gatgcgagca ctgaacctga actggaacct ggcccccgta ctcgacatca atcatcgcga
     2161 caaccctggt atcggcgccc gctcgttcgg tgaagatccg gaacgtgttt cccgtttcgg
     2221 ctgcgccgcg gtcgagggac tgcaggccgg tggggttctg gcctgcgcca aacattttcc
     2281 ggggaaaggt cacgcgcgcg tcgattcgca tttgactctg ccgaccattc cgtacgacac
     2341 ggcacgactc gaatcctttg agctgcagcc gttccgtgcg gccattcgtt cgggtgtcgc
     2401 ctcgatcatg acctcgcacg tgttttttcc ggcctacgaa tccaccccca atctgccggc
     2461 tacgctgtca aaggcggttc tcactgatct gttgcgcggc aaactcggct tcacggggct
     2521 tctggtcacg gacgatctcg agatgggcgc gatcacggaa gcgtatggag ttgccgaagc
     2581 cggatttcgc agtttcatgg cgggggcgga tctgttattg atctgtcatg atcttgatcg
     2641 cgagcgttct tgtgccgaga aaattctgga cgaatgccga cacaaccctg acgtggaaaa
     2701 gcgaatcaac
//
LOCUS       DAORHD010000644         2709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44455_length_2709_cov_2.962698, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000644 DAORHD010000000
VERSION     DAORHD010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44455_length_2709_cov_2.962698"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PKO06_13420"
     CDS             <1..188
                     /locus_tag="PKO06_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70694.1"
                     /translation="NEYVQTCRVDENVRYGIHVKICLGLELTSYERDWLVRKGRTSAA
                     NLDRYCNQYYSNSHGLI"
     gene            complement(244..873)
                     /locus_tag="PKO06_13425"
     CDS             complement(244..873)
                     /locus_tag="PKO06_13425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV70695.1"
                     /translation="MKLGLIRHGETVFNREERMQGVRDIELSDLGRQQAIELGRLLRQ
                     EECAPPQLYSSPVKRALDTATLLGVTTNIIAHPGFRARSLGSLEGLTKSEIREKFPGA
                     FERLVEWDYLPPGANETLRDIYTRANTAVEELVQRESEADQELVIVVTHSGVLEALLR
                     GWMQISAGQKLPVPLKNAGVFWLERPESGSSWVFTGSLAVGEQGTLDLA"
     gene            complement(870..1697)
                     /locus_tag="PKO06_13430"
     CDS             complement(870..1697)
                     /locus_tag="PKO06_13430"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNV70696.1"
                     /translation="MFLVKTTEGDSRSIWLLGNVGLEPEPVTFESGLRCLHQKSLSLR
                     QRATETPEPFSDEDVAAMSVVPPFGGTKILCVGRNYKPHAEELGNPIPIEPLWFSKPP
                     SSLVPSGGTVVIPEEVGRIDYEGELALVVGRRCRRLREAEALSCLAGVTAALDVTARE
                     LQKKDGQWTRAKGFDTFCPLGPVLLPFTEAWLDARLTTTVNDRLVQDDRTSSLIFSVP
                     QLLAHLTACMTLEPGDVILTGTPAGVGPIHPGDRVSVKIEGPQTLTLSVSVESAPRT"
     gene            1786..2493
                     /locus_tag="PKO06_13435"
     CDS             1786..2493
                     /locus_tag="PKO06_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HNV70697.1"
                     /translation="MITGSPYRSYAFFLLFAGCTFLPLAAPCQETATETGHVATIYTT
                     SYSPKDYTSLIGKCALSEKLWINHLALYQGYVKNTNMLNEIIKPMLFNGRAETPEFAE
                     LKRRFGWEYNGMRLHELFFQNMGGTGDFPKQGKLRDAIDRQFGGYEIWLRSATAMAKM
                     RGIGWFVLYQDVGTGMLINCWIDQHDTGHLAGCKPLLVIDFFEHAYMLDYGLKKSDYF
                     TNLIKHIDWKVVDARFQ"
BASE COUNT          595 a          800 c          732 g          582 t
ORIGIN      
        1 ggaacgagta tgtgcagacc tgtcgggtag acgaaaacgt tcgctatggc attcatgtca
       61 agatctgcct ggggctcgag ttaacctcat atgaacgcga ctggctggtc agaaagggtc
      121 gcacctcagc ggcgaacctc gaccgttatt gcaaccagta ttacagcaat agtcacggtt
      181 tgatctgaac ccaactcgga ctctcgcggc tacgcacccc gcatcgggca cttcaccggc
      241 gtctcaggcg aggtcgagtg tgccttgttc gccaaccgcg agcgaacccg tgaagaccca
      301 ggaactgccg ctttctggtc gttcgagcca gaatacgccg gcatttttca gcggcaccgg
      361 cagtttctgg cccgcggaaa tctgcatcca gcctcgcaga agagcctcga gcactccgga
      421 gtgagtcacc acgatgacca actcctgatc ggcctctgat tctctttgta ccagttcttc
      481 aaccgccgta ttcgccctcg tatagatgtc tcgcagggtc tcattcgcac cgggaggcag
      541 ataatcccac tcgaccagac gctcgaaagc accagggaat ttctcgcgga tctcgctttt
      601 cgtcagacct tcaagactac ccagcgaacg agcgcgaaac ccagggtgcg cgatgatgtt
      661 cgtcgtcacc cccaagagag tcgctgtatc cagagcccgc ttgaccggac tggaatacaa
      721 ctgcggcggc gcgcattcct cctgtctcag cagtcgacca agctcgatgg cctgttgtcg
      781 accaaggtca gacaactcga tgtcgcgcac gccctgcatg cgttcttccc gattgaacac
      841 ggtttcgccg tgccgaatca agccaagttt catgtccgag gtgccgactc gacagagacg
      901 ctcagagtaa gagtctgagg cccttcgatt ttcacgctga cgcgatcgcc ggggtggatc
      961 ggaccaacac cggccggggt tcccgtgaga atcacgtcac cgggttcaag tgtcatacag
     1021 gcggtgagat gggccaaaag ctgcggcacg gaaaaaatca gagaactggt tcgatcatcc
     1081 tgaaccagac gatcgttcac ggttgtcgtc aggcgcgcgt ccagccacgc ctcggtaaat
     1141 ggcagaagca ccggccccag agggcagaac gtgtcgaatc ctttcgcgcg cgtccactgg
     1201 ccgtcctttt tctgcagctc gcgagcggtc acatccagag cggccgttac accggcaagg
     1261 caggagagcg cttctgcctc gcgcagacgt cggcagcgac gaccgaccac cagagccagc
     1321 tcgccttcat aatcgatgcg gcccacctct tcaggaatca caaccgttcc accggaaggt
     1381 accagtgagc ttgggggctt cgaaaaccac agtggctcaa tcggaatcgg attgccgagt
     1441 tcttcggcgt gaggcttgta gtttcttccc acgcacagaa ttttcgtgcc tccaaacggg
     1501 ggaacaacgc tcatcgcagc cacgtcttca tcggaaaagg gttccggggt ctccgttgcc
     1561 cgctgtcgca atgagagact tttctggtgc aggcatcgca acccgctttc gaaagtcacc
     1621 ggctcaggtt cgaggccgac attcccgaga agccagatag agcgagaatc gccttcggtt
     1681 gtcttcacca gaaacatggc accctcctga gttttcgggt tttcatcaag cccacacaat
     1741 gatacaattc ggtctgcacg aacacaaatg ccgggaggtc gctgcatgat cacgggttcg
     1801 ccatacaggt cgtatgcatt tttcctactg ttcgcagggt gtacattcct ccctctggcg
     1861 gctccgtgcc aggaaacagc cactgaaacc ggtcacgtcg cgacgatcta tactaccagc
     1921 tactctccga aagactacac ctcgctcatc ggcaagtgcg ccttgagcga aaagctctgg
     1981 atcaaccacc tcgcgttgta tcagggatac gtgaagaaca ccaacatgtt gaacgaaatc
     2041 atcaaaccga tgctgttcaa cggacgcgct gaaactccgg agttcgccga actcaagcgg
     2101 cggttcggct gggaatacaa tggcatgcga ctgcacgagt tgttttttca gaacatgggc
     2161 gggaccggtg attttcctaa acagggaaag ttgcgcgatg ccatcgatcg ccagttcggc
     2221 gggtatgaga tctggttgcg ctcagccacg gccatggcca aaatgcgagg catcggatgg
     2281 tttgtattgt accaggacgt tggcacggga atgctgatta actgctggat cgaccagcat
     2341 gataccgggc accttgccgg ttgcaagccg ctgctggtca tagatttttt cgagcatgcc
     2401 tatatgctcg attacggtct gaaaaagtct gactacttca cgaacctgat caagcacatc
     2461 gactggaagg tcgtggacgc gcgttttcag taacgatcca acctgacccg aggctcgtcc
     2521 cagcgctttt tgcgccactc cgcgaacgct ggtatactca tatcagagaa ctcatcggat
     2581 ctttttttcg aggacgacgg tctcaacgtc gtcgcacccc tcattgcagg agtccgcgtg
     2641 tcaatgaacg tctgttttcg gcggggtttc tcgctcgtcg aggtcgtcat cgtcgcggct
     2701 attatcgcc
//
LOCUS       DAORHD010000645         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44505_length_2707_cov_3.649698, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000645 DAORHD010000000
VERSION     DAORHD010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44505_length_2707_cov_3.649698"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKO06_13440"
     CDS             complement(<1..907)
                     /locus_tag="PKO06_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HNV70698.1"
                     /translation="MSWWEAFFHPQYLDLVWFCLVGVISSCLFSFLPALHIYNVIGIF
                     MWFMLQFESMLEPMQLVAICMGMIVGYSILNTVPSTFFSPGDESLSYYVLPGAQWLAQ
                     GKGWEAVMLTGMGAVGGLVVLALLAPLLISILPGLRAITAPHMFWIIGAVISFMVLSE
                     WPKGTSQSPSCWANLWEGWKYLGVGLITFVLAALLGFIVTSRTFVPVEFAFQSITPVF
                     VGLFALPWLITNLICLGRIPRQHVSESVDLDWELFARGTFSGFLGGFTAAYVPIVTAG
                     VGGLMSGHGTAQRDSRLFVMSFGTCK"
     gene            complement(904..2154)
                     /locus_tag="PKO06_13445"
     CDS             complement(904..2154)
                     /locus_tag="PKO06_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70699.1"
                     /translation="MNLPPSASEEQKLYHEQTHCQSCGRFVGVFDRCPYCQAVTTKRL
                     SLRVFKLLSVLVSTVGLALLLFYARTIQPPLITIDSLTPLTNFAQVRIRGTVERSFGI
                     HPQWGSLSFVLAQKGKTGEVNRIRVSAYGKVASEVAALNNVPQEGDELTVQGLVRFQK
                     DNPGILLNSGRRIEFHKRASAAGAASAAPLSSSEVKPEMVGKTVQVKGKVAEVLTFPR
                     GILIRLDQRREGLPVWVPSRVHEDMEIDLSTLDEDDEIIVSGKVKPYKQDVEIEVAAA
                     NAIRRLGEPKPGTKATPAAPGAKETATVAPASATVEPLAKPPAETPKTEPKPVVAPAP
                     AAEPTPSPAPSTAPVDTSVPPAVSPVTVPAPAASNADPKPDALPTNLTPAPGVAPVTP
                     IAPAMAPAADAPAPASASTTGGTP"
     gene            complement(2190..>2707)
                     /locus_tag="PKO06_13450"
     CDS             complement(2190..>2707)
                     /locus_tag="PKO06_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70700.1"
                     /translation="NGATVVMPEIGVSQVAPKVMGRHLAIIAGREFDDFLRPLVRGFM
                     RGGGKVTRLMPNPRDEYTHAMPTAQANQIKDLDLLLSFRKGPAGRVSVRHYYRSDKGK
                     AFGRQLQKQWTQQTKGVRPPVPFVVEGGGEYELSHTGAPAVVFALPTTEEITAMVQLR
                     SILLDALKQMF"
BASE COUNT          573 a          821 c          828 g          485 t
ORIGIN      
        1 tcttgcaggt gccgaacgac atgacgaaga gccggctgtc tcgctgcgcc gttccatgcc
       61 cggacatcaa accgcccacg cccgccgtga cgatcggcac gtaggccgcc gtgaagccgc
      121 ccagaaagcc gctgaatgtg ccgcgggcga agagctccca gtcaaggtct accgattccg
      181 agacatgctg acgcggaatg cgtccgaggc agatcaggtt ggtaatcagc cagggcaggg
      241 caaacaagcc gacgaacacc ggcgtgatgc tctgaaacgc aaattcgacc ggaacgaagg
      301 tgcgggaggt cacgataaac cccagcagag cggcgagaac aaaggtgatc aaaccgacgc
      361 cgaggtattt ccagccttcc cagagattcg cccagcagct cggagactgc gaagtcccct
      421 tcggccactc ggacaaaacc atgaacgaga tgaccgcgcc gatgatccag aacatatgtg
      481 gagcggtaat ggcgcgcaat cccgggagaa tcgagatcag cagaggggca agcagcgcca
      541 gcacgaccag accgccaacg gctcccatgc cggtcagcat gacggcttcc cagcccttcc
      601 cctgcgccag ccattgagcg ccgggcagaa cgtagtaaga aaggctttcg tcgccggggc
      661 tgaagaacgt cgagggaacc gtgttcagaa tcgagtagcc gacaatcatg cccatgcaga
      721 tggcgaccag ctgcatcggc tcgagcatgg attcaaactg cagcatgaac cacatgaaga
      781 tgccgatcac gttatagatg tgcaacgccg gcagaaacga aaacagacac gacgagatga
      841 cgccaaccag gcagaaccag accagatcaa gatattgcgg atggaagaac gcttcccacc
      901 agctcatggt gtgcctcccg tggtggatgc actcgccggg gccggagcat cggcggcagg
      961 agccatggcg ggcgcaatcg gcgttacggg cgcgacgccg ggtgccggcg tcagattcgt
     1021 cggcaaggca tctggcttcg gatcagcatt cgaagctgca ggagccggta ccgtcacggg
     1081 cgacactgct ggcggcacgg acgtatcgac cggagcagta ctcggcgccg gcgacggggt
     1141 cggctcggcc gcgggtgcgg gtgcgacaac cggcttcggc tcggttttcg gcgtttccgc
     1201 cggtggcttt gccaggggct cgaccgtggc cgaagccgga gcgaccgtcg cagtctcttt
     1261 cgcgcccggt gctgcaggcg ttgccttggt tccgggcttg ggttccccaa gtcggcgaat
     1321 cgcgttggcg gctgccacct cgatctcgac atcctgtttg tacggtttca cttttccgga
     1381 cacgatgatc tcatcgtctt catcgagggt actgagatcg atttccatgt cttcgtgcac
     1441 gcgtgacggc acccagaccg gcaacccttc gcggcgttga tcgagacgaa tcagaatgcc
     1501 acgcggaaag gtcagcactt cggcgacctt gcccttgacc tggacagtct tgccgaccat
     1561 ctcgggcttc acctcggagg agctgagcgg agcggcactc gcagcacccg ctgccgatgc
     1621 tcgtttatgg aattcgatgc ggcgtccgga gttgagcaga atccccgggt tgtctttctg
     1681 aaagcgcacg agaccctgca ccgtcagttc atcgccttcc tgcggaacat tgttcagagc
     1741 cgcgacttcc gaagcgactt tgccgtacgc cgagacgcga atgcggttca cttcgccggt
     1801 cttgcctttt tgagccagga caaaactcag agaaccccac tgaggatgaa tgccgaaact
     1861 tcgctcaacc gtgccgcgga tgcgtacctg agcaaagttc gtcagaggcg tcagcgagtc
     1921 gatggtgatc aggggcggct ggatcgtgcg cgcatagaac agcagcaaag ccaaccccac
     1981 cgtcgagacg agcacggaca gcagtttgaa aacacgcagc gagaggcgct tggtggtgac
     2041 agcctggcag tagggacagc ggtcgaaaac accgacaaac cgaccgcagc tctgacaatg
     2101 cgtctgctcg tgatagagtt tttgttcttc cgaggcgctg ggagggagat tcatgtcgct
     2161 ttccttgagt agtttcgttg cgtcgatctt cagaacatct gcttcagggc atcgagcaga
     2221 atggaacgga gttgaaccat ggcggtgatc tcttcggtcg tcggcagggc aaagaccacg
     2281 gccggagctc ccgtgtggct cagttcatac tcgccaccac cctcgaccac gaaaggaacc
     2341 ggcggtctca ctcctttggt ctgctgggtc cattgtttct gcaactgtct gccgaacgcc
     2401 ttgcccttgt cactgcgata gtagtgcctg acactcacgc ggccggcagg ccccttgcgg
     2461 aacgaaagca gcagatcgag atctttgatc tggttggcct gggcggtcgg catggcatgc
     2521 gtatactcgt cgcgcggatt cggcatcaac cgggtgacct tgccgccccc gcgcatgaag
     2581 cctcgcacca gagggcgcag aaaatcgtca aactcgcggc cggcaatgat cgccaggtga
     2641 cgacccatga ccttcggcgc gacctgactc accccgatct cgggcatcac gaccgtcgcc
     2701 ccgttcg
//
LOCUS       DAORHD010000646         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44554_length_2705_cov_3.449434, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000646 DAORHD010000000
VERSION     DAORHD010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44554_length_2705_cov_3.449434"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PKO06_13455"
     CDS             complement(<1..195)
                     /locus_tag="PKO06_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNV70701.1"
                     /translation="MTESSQNQCGNRLSREKSPYLRQHASNPVDWYPWGEEAFARARE
                     EHKPIFLSIGYSTCHWCHVME"
     gene            complement(325..1470)
                     /gene="wecB"
                     /locus_tag="PKO06_13460"
     CDS             complement(325..1470)
                     /gene="wecB"
                     /locus_tag="PKO06_13460"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNV70702.1"
                     /translation="MSPSRLRLLFVLGTRPEVVKLAPVIRAARTDSRFSATLLHSGQH
                     LELADEMFRLFDLKPDISLSVMEPNQSLFSVSARILERLAPVFATDRYDMIVIQGDTT
                     TAFLGALAGYYTKTPVAHVEAGLRTFDRFSPFPEEMNRRLAGALTELHFPPTEAARQN
                     LLREGFDDNMITVTGNTVIDALLWALELPYQPSGDIREILSGSHRLVLVTSHRRESFG
                     EPHRRVFKALRTLADKHADIRLLFPVHPNPNVRAQVAELLGHHPRITLCAPLGYLDFI
                     NAMKRATLILSDSGGVQEEAPTLKKPVLVLRDHTERPEGLSSGALRLVGTDDQKILTE
                     ADRLLTDPAAYQAMTKNPNPYGDGKASERILDAISRYFSKRESQTRA"
     gene            complement(1493..>2705)
                     /locus_tag="PKO06_13465"
     CDS             complement(1493..>2705)
                     /locus_tag="PKO06_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70703.1"
                     /translation="SRVRDLFEQARKNAPCIVFIDEIDAVGRQRGAGLGGGHDEREQT
                     LNQLLVEMDGFDTGKGIILMAATNRPDVLDPALLRPGRFDRRIVVDQPDISGREQIIK
                     IHVRDKPLSKDVDASVLARRTPGFVGADLANLANEAAILAARHNRKIITMNDFEEAID
                     RVIAGAERKSRILSEKEKENTAYHEMGHALVAINLPGTDPVHKVTIIPRGMALGITMQ
                     LPLEDRFTVSRTLLIHQICILMGGRIAEEIVFGEVTSGAKNDIERATKIARKMVCELG
                     MSEEIGPLSLGDDENHAVFLGRDFARRRDVSEEMAKKIDSEIRRIIDNSYQKARQILL
                     DQKAQLIRISGILLERETLSGNDLKELLEGRELPPLRPPSETPSAPATEEPKSPEIAP
                     PTGEPSPTPAV"
BASE COUNT          498 a          774 c          872 g          561 t
ORIGIN      
        1 ttccatcacg tgacaccaat gacaggtcga atacccgatc gagagaaaga tcggcttgtg
       61 ttcttcgcgt gcccgcgcaa aggcttcctc gccccatgga taccagtcga ccgggttgct
      121 ggcatgttgc cgcagatacg gacttttctc gcgcgacagg cggttgccgc actggttctg
      181 agaactctct gtcatgcgct ggcctcccga tcattcttca cccggttcat ggtagcataa
      241 gtgaaccacg aggaatacat ggttttccct gttgtcacac aaggttaacc agggggaaaa
      301 gctggcggaa agagacggga gtcgtcaggc gcgggtctgg gattcgcgct ttgaaaagta
      361 ccgggagatc gcgtcgagaa tgcgctctga ggctttgccg tcgccgtagg ggttcgggtt
      421 tttcgtcatt gcctgatacg ccgccgggtc ggtcagcagg cggtcggctt cagtcagaat
      481 tttctggtcg tcggttccga ccagcctgag ggctccgctc gacagccctt ccgggcgctc
      541 ggtgtggtcg cgcagcacca gtactggttt cttcagtgtc ggagcttctt cctgcacccc
      601 gcctgagtca ctgagtatca aggtcgcccg tttcatggcg ttgatgaaat cgagataccc
      661 gagcggggcg cagagcgtga tgcgcgggtg atggcccagc agttcggcca cctgcgcccg
      721 cacgttcggg ttcgggtgca ccggaaacag caggcgaatg tcggcgtgtt tgtccgccag
      781 ggtgcgcagg gccttgaata cccgtcggtg cggttcaccg aaactctcgc gacgatgact
      841 ggtgaccaac acgagtcgat gagacccgct cagaatctcg cgaatgtcgc ccgacggctg
      901 gtacggaagc tcgagggccc agagcagagc gtcgatcacg gtattgccgg tcacggtaat
      961 catgttgtcg tcaaagcctt cgcgcagcag gttctgacgc gcggcttctg tgggcggaaa
     1021 atgaagctcg gtcagcgcgc ccgcgagccg gcggttcatt tcctcaggga acgggctgaa
     1081 acggtcgaag gtgcgcaggc ccgcttcgac atgcgcgacc ggggttttgg tgtagtaccc
     1141 ggccagcgcg ccgagaaagg cggtcgtggt gtcgccctga atcacgatca tgtcataacg
     1201 atcggtggcg aatacgggag ccagacgctc gagaatgcgc gctgaaaccg aaaacagcga
     1261 ttgattcggc tccatgaccg agagcgagat atcgggcttc aggtcaaaga gccgaaacat
     1321 ctcgtcggcc agttcgagat gttgccctga gtgcagcagg gtggccgaaa accgggagtc
     1381 tgtccgggcg gcccgaatga cgggcgcaag tttgacgact tccggacgcg ttcccagaac
     1441 gaaaagcagg cgcaggcgac tcggactcat ggagttgtcg tcgtgacgcg gatcagaccg
     1501 cgggagtcgg cgacggttcg ccggttggcg gagcgatctc cgggctcttc ggttcttcag
     1561 tggccggcgc cgaaggcgtt tcgctcggcg gtcgcagcgg cggcagttca cgaccctcga
     1621 gcagttcttt cagatcgttg cccgacaggg tttcgcgttc gagaagaatg cccgaaatgc
     1681 gaatcagctg cgctttctga tcgagcagaa tctgtcgcgc cttctggtac gagttgtcga
     1741 tgatgcgacg aatttcagaa tcgatcttct tggccatctc ttctgagaca tcgcggcgtc
     1801 gggcgaaatc gcgacccagg aacacggcat ggttctcgtc atcaccgagc gacagcgggc
     1861 cgatctcttc gctcatgccc agttcgcaca ccatcttgcg ggcaatcttg gtggcacgtt
     1921 cgatatcgtt cttggccccg ctggtgacct cgccgaagac gatctcttcc gcgatgcggc
     1981 cgcccatgag aatgcagatc tggtgaatca gcagcgtgcg cgagacggtg aatcgatctt
     2041 cgagtggcag ctgcatggta atgcccagcg ccatgccgcg tggaatgatg gtgaccttgt
     2101 gaaccgggtc ggtacccgga agattgatgg caaccagtgc atggcccatt tcatgatacg
     2161 cggtgttttc cttctctttt tcagacagaa tgcggctctt gcgttcggcc cccgcgatga
     2221 cgcgatcgat ggcttcctcg aagtcgttca tggtgatgat cttgcggttg tggcgagcgg
     2281 caagaatggc cgcttcattg gccaggttgg ccaggtcggc gccgacgaaa cctggcgtgc
     2341 ggcgcgccag caccgaggcg tcgacatctt tcgacagcgg cttgtcgcgc acgtgaatct
     2401 tgatgatctg ctcacgccca ctgatgtcgg gctgatcgac gacgatgcgg cggtcgaacc
     2461 ggccggggcg cagcagcgcc ggatcgagga cgtcaggccg gttggtggcg gccatgagaa
     2521 taatgccctt gccggtgtcg aacccgtcca tttcgaccag cagctgattc aacgtctgtt
     2581 cacgttcgtc gtgaccgccg ccgaggccgg caccgcgctg gcgcccgacc gcgtcgatct
     2641 cgtcgatgaa cacgatgcag ggggcgttct tgcgggcctg ctcgaacaga tcgcgcacgc
     2701 gtgac
//
LOCUS       DAORHD010000647         2705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44559_length_2705_cov_3.123019, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000647 DAORHD010000000
VERSION     DAORHD010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44559_length_2705_cov_3.123019"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKO06_13470"
     CDS             <1..696
                     /locus_tag="PKO06_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70704.1"
                     /translation="DLLRKAELLRKHRTPAEAGIVTPGASPAKQKQEIDDFLAKARAH
                     MQESLAVGKQASDRLTAGRKADLHQARQNGDANKAENLRITEALINTTNEAGIRVLSA
                     RDPGLMASVVPEAPKRSEASKPDLEPTTIGWGWFSERLLQKPDQSRVTLSPELQKTAD
                     RFQKIVDALEKSGLAGAKPGTREYRHLENLKNAAKAGASDPIAGLRQTRMISGFSSGQ
                     VLEELEKRVGVVK"
     gene            766..2133
                     /locus_tag="PKO06_13475"
     CDS             766..2133
                     /locus_tag="PKO06_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70705.1"
                     /translation="MQRSLMIGLTVFALLIANSGDVSGQNAQRGAWREKLQNRQQSGS
                     GQGNWKEKLQERIQNRQQGEGAEGSDWRDKLKEKWQNRQQDGAEGGTWREKLKEKWQN
                     RQQGEGAQGNWQERMQQWKAKWGEGNAEKMQAFRERWQQMQQANPQLQGFQERIKALR
                     EAKQSGDQSAFQQKLNELRSHFQGMNPEARASLQQNLPDVAKRLESLGAGQKQWNSQG
                     QVTGPAGNTSTYGGTTARNGNTWNQQGTWTGPKGRETQVEGTRTRDGNTVKEQKTWTG
                     EQGKQVTHQSTTTRDGNAVSTTGQWQGPQGGQVDVKGQTVRDGNTLTTDKTWTNQQGK
                     TVDLDRTTTKQGNQYQTTGQWTGPEGKTTTVQTTTTVDGKHVDKDVTVTGPDGKKTHL
                     DGDTNWSQGQKQTTWTNEDGKAAQHDVTWGKDGNQISRDSTWTGPNGNSADTSADWLI
                     DVLDE"
     gene            2156..>2705
                     /locus_tag="PKO06_13480"
     CDS             2156..>2705
                     /locus_tag="PKO06_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70706.1"
                     /translation="MKHSHLLKIIIVVLFLIAFSNSAWARSVSPSPTSDLPTLTVCGV
                     EIPPFILKHPYAYRLIFAKGDRAVTHASLLSDGRIRIDNIFHQNELWSADIDPLSIVA
                     AEVINQPIAPKVFHFGALFDFGDKGITLYQREGRATQIHELVMGTGPEKAFSIDGTIG
                     FQLLQMRASSLEAFQGKKPLEAK"
BASE COUNT          714 a          766 c          794 g          431 t
ORIGIN      
        1 gacctgctgc gcaaagcaga gctcctgcgc aagcatcgga cccccgccga ggcaggtatc
       61 gtgacccccg gggcttcacc agcaaaacaa aagcaggaaa tcgacgactt tctcgcgaaa
      121 gcccgtgcgc atatgcagga atcgctggcg gtcggcaaac aagcctcaga tcgtctgaca
      181 gccggtcgca aggccgatct gcatcaggcc agacaaaacg gagacgccaa caaggccgaa
      241 aatctgcgca ttacggaagc gctcatcaat acgaccaatg aagcgggaat tcgtgttctt
      301 tccgctcgcg accctggtct gatggcctcc gtcgtgccgg aagcgccgaa gcgttctgag
      361 gcatcaaagc ctgatctgga gccaacgacg atcggctggg ggtggttttc tgagcgacta
      421 ttgcagaaac ccgatcagag ccgagttacg ctgtctccgg aacttcagaa gacggccgat
      481 cgttttcaga agatcgtcga tgccctggaa aagtcaggtc tggccggtgc caaacccggt
      541 acccgagaat accgacatct tgagaacctt aaaaacgccg cgaaggccgg ggcatcggac
      601 cccatcgccg gcctcaggca aactcgcatg atcagtggct tctcttcagg tcaggtactc
      661 gaagaactcg agaagcgcgt cggtgtcgtc aaataggcag gacttcgtgt atcatggtca
      721 agtagtccgc acatcagcat ttcagcgctc cctcggagga caaccatgca gcgttctctc
      781 atgatcggtc tgaccgtttt tgctctactg atcgcgaatt ccggcgacgt ctcggggcag
      841 aatgcccagc ggggagcctg gcgggagaag ttgcagaacc gccagcagag cggctctggt
      901 cagggaaact ggaaggaaaa actgcaggag cggatacaga accggcaaca gggagaagga
      961 gctgaaggca gcgactggcg ggataaactc aaggagaagt ggcagaatcg ccagcaggac
     1021 ggggcagagg gcggcacttg gcgcgagaaa ttgaaggaaa agtggcagaa ccgccagcaa
     1081 ggcgaaggag ctcagggcaa ctggcaggaa cggatgcagc agtggaaggc caagtggggc
     1141 gaagggaacg ccgaaaagat gcaggccttc cgcgagcgct ggcaacaaat gcagcaggca
     1201 aacccgcaat tacagggctt tcaggaacgt atcaaagccc tgcgtgaggc caaacaaagt
     1261 ggcgatcagt ccgccttcca gcaaaaactg aacgagctgc gcagtcactt tcagggaatg
     1321 aatcccgagg cgcgtgcttc cttgcaacag aatctccccg atgtggccaa acggcttgaa
     1381 tctctgggag ccgggcagaa acaatggaac agccagggtc aggtgacagg ccccgccggc
     1441 aataccagca cctatggcgg cacgaccgcc cgcaacggga acacctggaa ccagcagggc
     1501 acctggaccg gccccaaagg acgagagacg caggtcgagg gcacccgcac gagagacggg
     1561 aacaccgtca aggagcagaa aacctggacg ggagaacagg gcaagcaggt gacccaccaa
     1621 tcgacgacga ctcgtgatgg gaatgccgtg agcacaaccg gtcaatggca gggcccgcag
     1681 ggtggccagg tagacgtgaa gggccaaacc gtacgagacg ggaacacgtt gaccaccgat
     1741 aaaacctgga ccaatcagca ggggaaaacg gtcgatctcg atcgtacgac cacgaaacag
     1801 ggaaatcagt atcagaccac cggccagtgg accggcccgg aaggcaagac caccaccgtt
     1861 caaaccacca ccaccgtcga cgggaaacac gtcgacaagg atgtcacggt caccggccca
     1921 gatggcaaga aaacccacct cgatggtgat acgaactgga gccagggaca gaagcagaca
     1981 acctggacaa acgaagacgg aaaggctgca cagcatgatg tcacctgggg aaaagacggc
     2041 aatcagattt cgcgcgacag cacctggacc ggtcccaatg gaaactctgc cgacacctcg
     2101 gcggattggc tcatcgacgt tctcgatgaa tgagactcgc aaaggaaggc aaccaatgaa
     2161 acactcacac ctgctgaaga taatcatcgt cgtgctgttc ctgatcgcct tttccaactc
     2221 ggcatgggca cgatcggtca gcccgtcgcc gacctctgac ttgccgacac tgacggtgtg
     2281 tggagttgaa attcccccat tcattctcaa gcatccgtat gcctatcgtc tcatcttcgc
     2341 gaagggcgac cgggccgtca cccatgcgtc cctgctttcc gacggcagaa tccgcatcga
     2401 caatattttt catcagaatg aattatggtc ggcagatatc gaccctcttt cgatcgtcgc
     2461 ggcggaagtc atcaatcagc caattgcgcc aaaggtcttt cacttcggcg cgctgttcga
     2521 tttcggcgac aagggcatta cgctgtacca gcgcgagggt cgggcgacgc agattcacga
     2581 gctggtgatg ggcacgggac ctgaaaaagc tttcagcatc gacggtacaa ttggttttca
     2641 gctcctgcag atgcgagcca gttctctcga agcctttcag ggcaaaaagc ctctcgaagc
     2701 caaag
//
LOCUS       DAORHD010000648         2704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44579_length_2704_cov_3.380143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000648 DAORHD010000000
VERSION     DAORHD010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44579_length_2704_cov_3.380143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKO06_13485"
     CDS             complement(<1..337)
                     /locus_tag="PKO06_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70707.1"
                     /translation="MTLVEIVFVFFLGSMVLLFGSQISSRTSLHVRKGTEMLNVQAFL
                     DQVVERLRSDVRSLSQLVNCKDHELTFVTTKKGKNETLTYRYDPQAKTFIRRAPDGGE
                     RTFAGADQIE"
     gene            complement(403..879)
                     /locus_tag="PKO06_13490"
     CDS             complement(403..879)
                     /locus_tag="PKO06_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70708.1"
                     /translation="MIEVLIAVVVLATAMGPILFLTMRTTSQAYSTTKHLIAGQFAAS
                     LMDWLLVENSYEQCKKMIDQSGGKQFRILDDPLFQDALFIKPAMAATTGGQDKQDMEE
                     DLGRVLKSFHYEVSQKAGTDDKMVLVTVKVSWLMNEDQEASRQFLTRKAIKFRERL"
     gene            complement(908..>2704)
                     /locus_tag="PKO06_13495"
     CDS             complement(908..>2704)
                     /locus_tag="PKO06_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70709.1"
                     /translation="VVLASLSALPYPESVVVPGALRDLEVVGAIRITCTCEYFKRTYT
                     LNVEYPYKVVMRLMPILREFLLFADKMHLEQVQPIGPGDGINILFTRQGDNPVPVPPA
                     YQSFQGKPFLLSFNGAHAVNRDKVGRVYLGPDDRDLFLNLAGEVNFRQELYSELWQVP
                     PKSFAVNTANKPFQPVAVIKGKDGSDIQFRGLDVPLKHNQAAMGLLGFCEEVADTSQG
                     PFANSPLDVEDAIGPKDPALTELLKDRKRMALSTALKLFGPNLEMSLPPTTTYSGPAR
                     ELFGNVYNRYFIISMFIYPSQLGPQPLIYQTNANHQPDPFPRFFGYAPVPFEPQFGKY
                     GDYMSRVVSGGYDAMKDTWLPANKGGGSTIKCLTSSDFHPADNMSLNGRFDSFARDWL
                     TIDAAAAAPRSVQTRIGEYFKTQAEFKEYVGVGRTPPRFWVDGVHYIDEKLELPDLTL
                     PDAQIRGGIILVNGPITLGNVTRGLAVGIKADDPAVKKELDRLTQDHFLTFVSVSGDA
                     ITLTGDLHVGVHLISMKADVSARKDQLKWAKGENVRFFGGIAVNTLNLSERVKQFKQV
                     PRIEYPAITADPKPATTVQIAATLGGYQFYVP"
BASE COUNT          596 a          819 c          738 g          551 t
ORIGIN      
        1 tttcaatctg atctgctccg gcgaatgttc gttcgccgcc atcgggagcc cggcgaatga
       61 aggttttcgc ctgcggatca tagcggtagg tcagggtttc gttcttgccc ttcttcgtcg
      121 tgacgaaggt caattcgtgg tctttgcagt tgaccagctg cgagagactg cgaacgtcgc
      181 tgcgcagccg ctcgaccacc tgatcgagaa aggcctgcac attcagcatc tcagtgccct
      241 tccgtacgtg cagcgaggtt cgacttgaga tctgcgagcc aaagagcagc accatactgc
      301 ccagaaagaa aacgaagacg atttcgacca gcgtcacacc ccggcttccc tggccgccag
      361 gaaagcttga acgaggccag gaccagtgaa tgaaagatga cgtcacaatc gctcgcgaaa
      421 cttgatggct tttcgggtca gaaactgacg actcgcctcc tgatcttcgt tcatcagcca
      481 ggagaccttc accgtcacga gtaccatttt atcatcggtt cccgctttct gcgagacttc
      541 ataatgaaat gacttgagaa cccgccccag atcctcttcc atatcctgct tatcctggcc
      601 gccggtcgtc gcggccatgg caggcttgat gaacaacgca tcctgaaaga gggggtcatc
      661 cagaatccga aactgctttc cgcctgattg atcgatcatc ttcttgcact gctcatacga
      721 gttttcgacc aggagccagt ccatcagact cgcggcaaac tggcccgcaa tcagatgttt
      781 ggtcgtggaa tacgcctgcg aggtggtccg catcgtgaga aagagaatcg gccccatcgc
      841 ggtcgccagc acgacaacgg caatcaggac ctcgatcatg gtcagagccc tgcgcgtatg
      901 cgccacgtta cgggacatag aactgatacc ccccgagggt ggctgcgatc tgcaccgtcg
      961 tcgccggctt cggatcagcc gtgatcgccg gatactcgat gcggggcacc tgtttgaact
     1021 gcttcacgcg ctcggacaga ttcagggtgt tcacggcgat accgccgaag aagcgcacgt
     1081 tctcgccttt cgcccacttg agctggtctt ttctggcgct gacatctgct ttcatcgaga
     1141 tcaggtgcac ccccacgtgc aaatcgccgg tcaacgtgat cgcatcgccg ctgacactga
     1201 caaaggtcag gaaatggtcc tgcgtcagcc tgtcgagttc tttcttcacc gccgggtcat
     1261 cggccttgat accgaccgcg agaccgcggg tgacgttccc cagagtgatc ggcccgttca
     1321 ccagaatgat gccgccgcga atctgggcat ccggaagcgt gagatcgggc aactcgagct
     1381 tttcatcaat gtaatgcacg ccgtcgaccc agaaccgggg cggggtgcgg ccaacaccca
     1441 catactcctt gaactcggcc tgcgtcttga aatactcccc gatccgcgtc tgcaccgatc
     1501 tgggggccgc ggcggccgca tcgatcgtca gccagtccct ggcgaaggag tcgaaacgac
     1561 cattcagtga catattatcc gcggggtgaa agtcagagga ggtcagacac ttgatcgtgc
     1621 tcccaccgcc tttgttggca ggcaaccatg tgtctttcat cgcgtcgtat ccacccgaca
     1681 ccactcgcga catatagtct ccgtacttgc caaactgagg ttcgaacggc accggagcat
     1741 agccgaaaaa gcgaggaaac ggatccggtt gatggttggc gttcgtctgg tagatcagcg
     1801 gctgcggccc cagctgcgaa ggatagatga acatcgagat gatgaaatac cgattgtaga
     1861 cgttcccaaa caactcgcgc gccgggcccg agtaggtggt cgtgggcggc agactcattt
     1921 ccaggttcgg cccgaacagt ttcagcgccg tcgaaagcgc catgcgcttt cgatctttca
     1981 gcaactcggt gagggcggga tctttcggcc cgatggcatc ttcgacatcc agcggagaat
     2041 tggcaaacgg accctgactc gtgtccgcaa cctcctcaca gaaccccaac aaacccatgg
     2101 cggcctgatt atgcttcagc ggcacatcaa gcccgcgaaa ctgaatgtcc gagccatcct
     2161 tacccttgat gaccgccacc ggctgaaatg gcttgttggc ggtgttcacg gcaaacgact
     2221 tgggcggcac ctgccagagt tccgagtaga gctcctggcg aaaattcacc tcaccggcaa
     2281 ggttcagaaa caggtcacga tcatcaggac caagatagac acggcccacc ttgtcgcgat
     2341 tcacggcatg agccccgttg aatgacagca ggaacggctt tccctgaaag ctctgatacg
     2401 ccggcggcac cggcaccggg ttgtcgccct gacgggtaaa cagaatgttg atgccgtctc
     2461 cgggcccaat cggctgcacc tgttcgagat gcatcttgtc cgcaaacagc agaaactctc
     2521 gcaggatggg catgagacgc atgaccacct tgtacggata ctcgacattg agcgtatacg
     2581 ttcgtttgaa atactcacag gtgcaggtga tacgaatcgc ccccaccact tcgagatccc
     2641 gcaaagcgcc gggaaccacc accgattccg ggtacggcag cgccgagaga ctggccagaa
     2701 ccac
//
LOCUS       DAORHD010000649         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44622_length_2702_cov_3.193804, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000649 DAORHD010000000
VERSION     DAORHD010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44622_length_2702_cov_3.193804"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKO06_13500"
     CDS             complement(<1..964)
                     /locus_tag="PKO06_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV70710.1"
                     /translation="MREQIPNEKLQFVGKECERLDTIEKLTGMATYTGDMVLPGLLYA
                     KVKRSPHARARIARMDTSKARRLPGVHAILTGNDLDYRVGLYLVDKYIMARGVIRHFG
                     EAVAAVAAETPQLAQQAVDLIDIDYEVEEPALDPVKALEKGAPLVHPDLGSYDYFKGV
                     FTPQPGTNIANHMKIRKGDVEKGFAEADQIIEGEYTNPSVQHVPMETHVAIGRWEPND
                     KVTIWTSAQSPFTVRNLFCHTFKLPHNNVRVHIPYVGGGFGGKAGIHLEPLVCCLSRK
                     AGGRPVKMVATREEEFSMLPCRSGLVYKIKTGIRKDGKIVAQKMT"
     gene            complement(957..1442)
                     /locus_tag="PKO06_13505"
     CDS             complement(957..1442)
                     /locus_tag="PKO06_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV70711.1"
                     /translation="MKITMTVNGREYTRDVPGNRTLLRFLREDLGLTGTKEGCSVGEC
                     GACTVLVNNEPVNSCIALAAEYHGAIIETIEGEAADGRLSKLQQAFHRHHAVQCGFCT
                     PGMIMSVKALLRRTPKPTEEEVKEAVEGNFCRCTGYKQIFEAVLDVTGQLKKKEDLHH
                     A"
     gene            complement(1439..2287)
                     /locus_tag="PKO06_13510"
     CDS             complement(1439..2287)
                     /locus_tag="PKO06_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNV70712.1"
                     /translation="MLQEFSYQAPATQAELLQLLKERGNEVRLLAGGTDLLVDIRSGF
                     HKPQTLVDLKKIPELRQLTFDAKTGLSIGAAVRCIDVMQNKIVREKYPLIANAASRIG
                     SIQLRNRATLVGNLCTASPAADMAPGLLCLDASVEIASTTGTRTVKLSEFFTGVKRTV
                     LDQSEVVTRILVPATTAGLKSGMEKLKRIKGHDLALASVCMVRRDTRLRVAIGACSVT
                     PVLLPEFSVGDALSDIKEAAAKAIKPIDDVRASAKYRVFMVQTYIERLWNQIMKEETA
                     KGGKAK"
     gene            2409..>2702
                     /locus_tag="PKO06_13515"
     CDS             2409..>2702
                     /locus_tag="PKO06_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70713.1"
                     /translation="MNSVCRRILVSLLLIGAGALSPLGSLLACNPEPGPHHFTKISPP
                     PPALTVGYFEVFNTLKGLPCNAIRSILAGGDFTLVGTDGGGLMVYRQGSWVSFT"
BASE COUNT          488 a          761 c          826 g          627 t
ORIGIN      
        1 aggtcatttt ctgcgcgacg atcttcccat ccttacgaat gccggtcttg attttgtaga
       61 caagcccgga acgacagggc agcatcgaga actcttcttc gcgggtggcg accattttca
      121 ccggtcggcc gccggctttg cgcgagagac agcagaccag cggttcaaga tgaatgccgg
      181 ccttgccgcc gaatccgccg ccgacatagg gaatatgaac ccggacatta ttgtgcggca
      241 gtttgaaggt gtggcagaaa aggttgcgta cggtgaacgg gctctgtgcg gaagtccaga
      301 tcgtgacttt gtcgttgggt tcccatctgc cgatagcgac gtgggtttcc atcgggacat
      361 gttgtacgct tgggttggtg tattcacctt cgatgatctg atcagcttcg gcaaaccctt
      421 tctcgacgtc gcccttgcgg atcttcatat gattcgcgat gttggtgcct ggctgaggag
      481 tgaagacgcc cttgaagtag tcgtagctgc ccaggtcggg gtgtaccagt ggggcccctt
      541 tttcgagagc cttgaccggg tcgagcgcgg gttcttcgac ttcgtagtcg atgtcgatca
      601 gatcgaccgc ctgctgagcg agctgcggtg tttcggcggc tacggctgcg actgcctcgc
      661 cgaagtgacg aatgacgcca cgggccatga tgtatttgtc gaccagatac agaccgaccc
      721 gatagtccag gtcattgccg gtcagaatcg cgtgtacgcc aggcagacgg cgagccttcg
      781 atgtgtccat gcgcgcgatg cgggcgcggg cgtgcggact gcgcttgact ttagcataca
      841 gcaggccggg cagcaccatg tcaccggtgt aggtcgccat gccggtgagc ttctcgatgg
      901 tatcgagtcg ttcgcattcc ttgccgacga actgaagttt ttcattcggg atctgctcac
      961 gcatggtgca ggtcctcctt cttcttcagc tgaccggtca cgtcgagcac ggcctcgaag
     1021 atctgcttat agccggtgca gcggcagaaa ttgccctcga cggcttcctt cacttcctct
     1081 tcggtgggct tgggggtgcg ccggagcagg gctttcaccg acatgatcat gccgggagtg
     1141 cagaagccgc attgcaccgc gtggtggcga tgaaatgcct gctgcagctt cgacaagcgc
     1201 ccgtcggcgg cttcgccttc gatggtttcg atgatcgcgc cgtgatattc cgccgccagc
     1261 gcgatgcagg agttgacggg ttcgttgttg accagcaccg tacaagcgcc gcactcgccg
     1321 acgctgcatc cttccttggt tccggtcagt ccgaggtctt cgcgcagaaa ccgcagcagc
     1381 gttcggttac cgggaacatc gcgggtgtac tctctgccat tgacggtcat ggtgatcttc
     1441 attttgcctt gcctcccttc gcggtttctt ccttcatgat ctggttccag aggcgttcga
     1501 tgtaggtttg caccatgaac acccgatact tggccgaagc gcgcacatcg tcgatcggct
     1561 tgatcgcctt ggccgcggct tccttgatgt cagaaagcgc gtcaccgacc gaaaactcgg
     1621 gcagcagcac cggggtgacc gagcaggctc cgatggcgac gcgtagcctg gtgtcgcgac
     1681 ggaccatgca aacgctggcc aacgcgagat catggccctt gatgcgtttg agtttttcca
     1741 tgccgctttt cagaccggcg gtggtcgcgg gaaccaaaat gcgggtcaca acttcggatt
     1801 gatcaagcac cgtgcgcttg acaccggtga agaactcgga cagttttacg gtgcgcgtgc
     1861 cggtggtcga agcgatttcg accgaggcat cgagacacaa gagacccggc gccatgtcgg
     1921 cagcgggaga ggcggtgcag agattcccca ccagggtggc tcgattccgc agttgaatcg
     1981 agccgatccg actcgcggcg ttggcaatca gtgggtattt ttcgcgcacg atcttgttct
     2041 gcatgacatc gatgcagcgc accgcggcgc cgatcgacag acctgtcttg gcgtcaaagg
     2101 tcagttgccg gagttccgga atttttttca ggtcgaccag ggtctgaggt ttgtgaaacc
     2161 ccgatcgaat atcgacgaga aggtcggtgc cgcctgccag cagacgaacc tcgttccccc
     2221 gttctttgag aagttgcaga agctcggctt gagttgccgg ggcttgatag gaaaattcct
     2281 ggagcatgcc ctctcctttc cttgaaataa gtcgaacgat ttgttgaaat ggtagagaaa
     2341 atcggtcgcg ggtgcaactg tttctccgtt tgcggagaga ctgccgctgc acgaactggg
     2401 aggaacccat gaattcagtc tgtcgccgga tcctggtatc tctgctgctg atcggagctg
     2461 gagctctttc gccgctcggc tctcttctcg cgtgcaaccc tgagccggga ccgcatcatt
     2521 tcaccaagat cagcccgcca cctccggctc tcacggttgg atacttcgag gtgttcaaca
     2581 cgctgaaggg gctgccgtgc aacgcgattc gcagcattct cgccggtggc gatttcacgc
     2641 ttgtgggcac cgatggtggc gggctcatgg tgtatcgcca gggctcctgg gtcagcttca
     2701 cg
//
LOCUS       DAORHD010000650         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44638_length_2701_cov_3.891534, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000650 DAORHD010000000
VERSION     DAORHD010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44638_length_2701_cov_3.891534"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="PKO06_13520"
     CDS             complement(<1..2224)
                     /locus_tag="PKO06_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70714.1"
                     /translation="MIARRSTPDLPFLALCFCLLLTVLTGRAWLSDQAEKVRESWIGE
                     ERDGLRRILQNTLRLGSPQTCFAEIMRRTIRDLRAGRTLPDSFRRFRPWAGTQLELLV
                     FDADGRRLTMEGFRLERGAVSEKVLHALLEPAPTSTTNIRKLSASFLGMRTGLEKLAE
                     SPASMVELPGSTRYAYAGWWSVRDVGAPETGAAASTKVRVLKYDVLLLVPKKGLETPV
                     FLRRSVLNMRRLLHRHARLDLVRHADRSESAVAFSLSRRVARLDVRLACTNAMDLRIR
                     RRVQTPATIARMLNALEAAHLALVLGSLLVLAVGFRQFRPDEPERLTLGRLIRLGLLG
                     SVIPALTMFLTWTFVFLHTSSKTMSWQAHRKLEHELERIDRDSSLERRRVDVRLNRMI
                     DGVQGLSDSEAAKKIGVELEGCPQVEAAFLIEPYGKVLATPRRHRSTHETQRVFALSV
                     AVNLTAVLENREPKSICRESFDLTFVKGFTRYLLENFGRTEELTGFGMRRSVFVRLVR
                     GETSTTPRYFVALLNLDDLQKQYVRNMLAQHSRECWLGMVETGPAGPNRGYPERLLWN
                     PDLLSLVEEAHRLRRTTDVRLQLPRRGTYLASAIPGRQLSGHVLVGMVPYQPIDQRIR
                     LWLGTAVFALVGIIILGVGAALALAGYLRTGMHRLEDGLYNLQHRHFELSPVSVDDQL
                     SCIYQGMVTAGRTLDELYAAAPVQKTLVHEGTTRKAGLLISSYFQAGPFLGGDFADVL
                     E"
     gene            complement(2221..>2701)
                     /locus_tag="PKO06_13525"
     CDS             complement(2221..>2701)
                     /locus_tag="PKO06_13525"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV70715.1"
                     /translation="HGAGSSMGETIRIALTTVAEIIGLRHRILRAGLPLETACLHGDQ
                     DESTRHLGLWGGETLLGCVSLMVAPCDGLPAMQLRGMAIAREAQGRQYGRLLLHEAEQ
                     LVAQVGFSLIWCNARETARGFYARHGWQVTSAPYFIEHAGMHVRMQKSLAAANSGGA"
BASE COUNT          576 a          868 c          766 g          491 t
ORIGIN      
        1 gttcgagaac atcagcaaag tccccgccga gaaaggggcc ggcctgaaaa tagctgctga
       61 tcaacagtcc cgctttgcgt gtcgtccctt cgtggaccaa cgttttctgc accggagccg
      121 ccgcatacag ttcatcgagc gttcgtccgg cggtgaccat gccttgatag atgcaggaaa
      181 gctggtcatc aacggaaacg ggggataact cgaaatgccg atgctgcagg ttgtacaacc
      241 cgtcctcgag tcgatgcatg ccggtacgca ggtatccggc cagtgcgagc gcagccccga
      301 cacccaggat gatgataccc acgagcgcga acactgccgt acccagccag agtctgatac
      361 gttgatcgat gggttgatac ggcaccatcc ccaccaacac gtgaccactc aactgcctgc
      421 cgggaatggc ggaggcgaga tacgtgccgc gccgcggcag ctgcagacga acgtcggtgg
      481 ttcgacgcaa tcgatgcgct tcctcgacca gactcaacag gtcaggattc cagagcaggc
      541 gttcggggta gccacgattc ggcccggcgg gaccggtttc caccattccc agccagcact
      601 ctctcgagtg ttgtgccagc atattgcgca catactgttt ctgcagatca tcgagattca
      661 gcagggcgac gaagtagcgt ggagtcgtac tcgtctcgcc gcgcaccagc ctgacaaaaa
      721 cacttcttct catgccaaac ccggtcagtt cttccgtccg tccgaagttt tcgagcaggt
      781 accgggtgaa gcccttcaca aatgtcagat cgaagctttc gcgacagatg gacttcggtt
      841 cgcggttctc gagcaccgcc gtcagattca ccgcgaccga cagcgcgaac acgcgctgcg
      901 tctcgtgcgt cgaccggtgg cgtcgtggcg tcgccaggac tttgccgtag ggttcgatca
      961 gaaacgcggc ttcaacctgc ggacacccct cgagctcgac gccgatcttc ttcgcggctt
     1021 ctgaatcaga caagccctgc actccgtcga tcatccggtt caaccggaca tcgactcggc
     1081 gccgctccag actgctgtca cgatcgatcc gttcaagctc gtgctccagc tttcgatggg
     1141 cctgccaact catcgttttg ctcgacgtgt gcagaaacac gaacgtccag gtgagaaaca
     1201 tcgtcaacgc gggaatgacc gagccgagaa gacccagcct gatcaggcgt cccagggtca
     1261 ggcgctccgg ttcatccggt ctgaactgtc gaaaaccaac tgcgagtacc agcagacttc
     1321 caagaacaag agcgagatga gcggcttcca gagcattcaa cattcgcgca atggtcgccg
     1381 gagtctgcac gcgtctacga atacgcaggt ccatggcgtt cgtacaggcc agacgaacat
     1441 cgagccgggc gactcgccga gacagcgaga acgcgacagc cgactcagat cgatccgcgt
     1501 gccgcacgag atccagtcgg gcgtgacgat gcagcagacg cctcatattc aaaacgctgc
     1561 gtcgaagaaa caccggggtc tcgagcccct tcttcggcac cagcagcaac acatcgtatt
     1621 tcaacactcg gactttcgtg cttgcggcgg cacccgtttc aggagcaccg acatccctga
     1681 cagaccacca gccggcatag gcatatcgcg tcgaacccgg cagttccacc atgctggccg
     1741 gactctccgc gagtttttcc agcccggtac gcatgccgag aaagctggca gagagcttgc
     1801 gaatgttggt cgtcgaggtt ggagccggct cgagaagagc gtgcagaacc ttctcggaga
     1861 cagcccccct ttcgagacga aacccttcca tcgtgaggcg acgaccgtcg gcatcgaaca
     1921 ccagaagctc cagttgagtg cccgcccacg gccgaaaacg gcgaaaacta tcgggcagag
     1981 tcctccctgc tctcaagtca cggatcgttc gtcgcatgat ttccgcgaag cacgtttgcg
     2041 gagagccgag tcgcagggtg ttctgcagaa tgcgccgcag accgtcgcgt tcctcgccga
     2101 tccagctttc gcgcactttc tccgcctggt cgctcagcca cgcccgcccc gtcagaacgg
     2161 tcagcaacag gcaaaagcag agagcgagaa agggcagatc aggagtcgat ctgcgagcta
     2221 tcatgcgccg ccgctgttcg ccgcggccag cgatttctgc atgcgcacgt gcattccggc
     2281 gtgttcgatg aaatacggtg ccgacgttac ctgccagccg tgcctggcgt aaaaaccgcg
     2341 ggctgtctca cgcgcattgc accagatgag agagaatccg acctgcgcga ccaactgctc
     2401 ggcttcgtgc agtaacagtc tgccatactg ccgaccctgc gcttcccggg cgattgccat
     2461 tccccgcagc tgcatcgccg gcagaccgtc acagggagca accatcagcg agacgcaccc
     2521 gagtaaggtt tctccgcccc agaggccgag atgccgcgtc gattcgtcct ggtcaccatg
     2581 caggcacgcg gtctccaaag gcagaccggc gcgcagaatg cgatgccgca ggccgatgat
     2641 ctcggccacg gtggtcaaag cgatccgtat cgtttcaccc atgctcgagc cggctccgtg
     2701 g
//
LOCUS       DAORHD010000651         2700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44674_length_2700_cov_2.254064, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000651 DAORHD010000000
VERSION     DAORHD010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44674_length_2700_cov_2.254064"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKO06_13530"
     CDS             complement(<1..188)
                     /locus_tag="PKO06_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70716.1"
                     /translation="MKRHDSAFSLMELMVVLFILQLVICPLYVVFSGSRKIMMSARDV
                     TSAVSLGSSMIAALREIDR"
     gene            complement(172..615)
                     /locus_tag="PKO06_13535"
     CDS             complement(172..615)
                     /locus_tag="PKO06_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70717.1"
                     /translation="MTTFLVSLVLAFTMKLFSAASSQSSDLEMRQMSETEKQRLLTAL
                     RADCRSAKSVLATPEAVEIVRFQFDQANNITEQHVAYLYVAGNIVRHEGDVQKAFEFL
                     RYEKQGSKLQFRFETASPATLLLYLKNETTDVVIMNEKIVYETTR"
     gene            complement(667..1494)
                     /locus_tag="PKO06_13540"
     CDS             complement(667..1494)
                     /locus_tag="PKO06_13540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV70718.1"
                     /translation="MRSRMYRDSEYEYASSSWSEDAVNSLWQSANGRVSLVLIALVFL
                     VSLSGCGKTEVSPAEAKITKTLGVVQWKSDSQTTWTTAKVGDRLAIGGCLKTGEEAAA
                     SLELPGGHQLHFAQDTFFEYREPAEGVRQAGGKIEYHVIKESGKDFKVRTPHAQTAVL
                     GTTFFVSVLATSTEVALIEGKLRVANLKGDIEKTMISGQKARVLQESIDLSSGTVRET
                     RPGSPPSIPGDQMRNPEPQNNTTPQNASGTIDLHGNQVQDSPGDQSIRKLVEPGKGD"
     gene            complement(1883..>2700)
                     /locus_tag="PKO06_13545"
     CDS             complement(1883..>2700)
                     /locus_tag="PKO06_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HNV70719.1"
                     /translation="NVEDAADQLVIAKWVVREVAYKHNLEVSFAPKIIVGHAGSGLHI
                     HSRLVKDGRNMMVDETGSLNDTAKKMIAGLLECAPSITAFGNIVPTSFLRLVPHQEAP
                     TNICWGDSNRSVLVRVPLGWKGVSNMIYDANPQEKEPYNDPMANQTVELRSADGSANV
                     YQLLAGICVAARHGLENPNALELAKKLYVSLDVGKNQKELNLPQLPASCCDAADQLVR
                     QREIYEKYGVFPASLIDGVAKQLKSFNDKDLSERMFGDGDALMHLVNQYLHCG"
BASE COUNT          578 a          750 c          729 g          643 t
ORIGIN      
        1 cgatcaatct cgcgcaaagc agcgatcatc gatgagccga gactcacagc actggttacg
       61 tcgcgcgcac tcatcatgat cttcctgctt cctgaaaaaa cgacgtacag agggcagatg
      121 acgagctgta gaatgaacaa gaccaccatc agctccatca gggaaaaagc actatcgtgt
      181 cgtttcatag acgattttct cattcatgat gacaacatcc gtcgtctcat ttttcaagta
      241 cagcaggagc gttgccggag aagcggtctc aaaacggaat tgaagctttg atccctgttt
      301 ttcatatcgt aggaactcga acgccttctg cacatcacct tcatgacgaa cgatgtttcc
      361 agcaacatac agataggcga cgtgttgttc cgtaatattg ttggcctgat caaactggaa
      421 acgcacgatt tcgacggctt caggagttgc cagcacggat ttcgccgaac ggcagtctgc
      481 ccgcagcgca gtgagtagtc gctgcttttc cgtttccgac atctgccgca tttccaggtc
      541 gctcgattga gacgaggccg ctgaaaacag cttcatggtg aaggcaagaa ccagagaaac
      601 cagaaacgtt gtcatcagaa tctcgatgag agtcaggcca cgtgtccggg aacgcctcga
      661 atacacctag tcccccttgc ccggttcaac cagctttcga atggattgat ctcccggcga
      721 atcctggacc tgattcccgt gaagatcgat agtcccactc gcattctgtg gtgtcgtatt
      781 gttctgtggt tcaggatttc tcatctgatc tcctggtatg gaaggcggtg acccgggcct
      841 cgtctcgcgc accgtgcctg aacttaagtc gatcgactcc tggaggacac gagccttttg
      901 cccggaaatc atggtctttt cgatatctcc tttcaggttg gcgaccctca gtttcccttc
      961 gatcagggcg acttcggtgc tggtcgccag gacggacacg aaaaaggtcg ttcccagaac
     1021 agccgtttgc gcatgcggcg tacgaacctt gaagtccttt cccgattcct tgatgacatg
     1081 atactcaatt ttgcccccgg cctgtcgaac tccttcagcc ggttcgcgat actcgaagaa
     1141 cgtgtcttga gcgaaatgca gttggtgacc accgggcagt tccagtgacg cagcagcttc
     1201 ctcgcctgtc ttgagacacc cccctattgc caggcgatca ccgacctttg ccgtggtcca
     1261 tgtcgtctgt gaatcacttt tccactgaac caccccaagt gtcttggtga tcttcgcttc
     1321 ggccggtgat acttctgttt tgccgcaacc actgagagag acgaggaaaa ccaaagcgat
     1381 gagtaccaat gataccctgc cgttcgcaga ctgccacaga gaattcaccg catcttcgct
     1441 ccatgaactg cttgcatact cgtactcgga gtcgcgatac attcgtgatc tcattcgttt
     1501 cccccttgag acgtcgtttc actggcagac tgaagcagct gtttcaagct gccggacggc
     1561 ggtgttcccg ctgtgagagc tggaacgggg acgacacttt cggtggcgga aggaatcact
     1621 gggtgatcgg agtcaagcat gaacccatca tgaccgtgct cgctattcga cgaagggaac
     1681 aagaaaagac tatcatgagc ctgtgtagct gtgaagagaa atgtgatctg ccgagtaacg
     1741 atttttctgg caacaatcca taccggttga tcgttcgcgt gcgacaccga cgcgaaaaac
     1801 aagaactccc tgaacgctga cacgcgttca gggagttcgc tttgttcagt cgtccgagtt
     1861 gtcgaacggt gatccgctga tctcagccgc agtgcaggta ttgattgacg agatgcatga
     1921 gagcgtctcc atcgccgaac atgcgttcac tcagatcttt gtcgttgaag gacttcagtt
     1981 gtttggcgac gccgtcgatg agagaagccg ggaagacacc gtacttctcg tagatctcgc
     2041 gctgtcgtac cagttggtcg gccgcatcgc agcacgaggc gggcagttgc ggcagattga
     2101 gttctttctg gttcttgccg acgtcgaggc tcacgtagag cttcttcgcc agttcgaggg
     2161 cgttcgggtt ttcgagaccg tggcgcgcgg cgacgcagat acccgccagc aactggtaca
     2221 cattcgccga cccgtcggca ctgcgcagtt cgacggtctg attcgccatc gggtcgttgt
     2281 atggttcttt ctcctgaggg ttggcatcgt agatcatgtt gctgacgcct ttccagccca
     2341 gcggaactcg gaccagcacc gaacggttgc tgtcgcccca gcagatgttg gtcggggctt
     2401 cctgatgcgg cacgaggcgc aggaatgagg tcggcacgat gttgccaaag gccgtgatcg
     2461 aaggcgcgca ttcgagcaga cccgcgatca tcttcttcgc cgtgtcattc agcgaacctg
     2521 tctcgtcgac catcatgttg cgaccgtcct tcaccaggcg ggaatggatg tgcaggccac
     2581 tgccggcatg gccgacgatg atcttgggcg caaagctcac ttcgaggttg tgcttgtagg
     2641 cgacttcgcg aacgacccac ttcgcgatga ccagctgatc ggcggcgtct tcgacgttgg
//
LOCUS       DAORHD010000652         2694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44808_length_2694_cov_3.441455, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000652 DAORHD010000000
VERSION     DAORHD010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44808_length_2694_cov_3.441455"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKO06_13550"
     CDS             complement(<1..1043)
                     /locus_tag="PKO06_13550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV70720.1"
                     /translation="MKKSLGLGILAVLLVGFVAASAWRYLAIRERQAQAPSMPTIAPL
                     IRVKTIETGQARKTVPVLATLKSRASIRLMPEVAGRLTMLRKREGDVVKAGEVIARLE
                     SDELQTQLRMAGAQSQAVGRQTAAAAESIRSLTSQLPALKANMSYWQAEATRDRTLYE
                     RGALSRAQYEGTENRLAEAKAKVTALEAQIDAARAQMQAVASQKDAAAHTVSLWQVRN
                     RYAELKAPVDGVISGRHQEEGNYVTSSTAIYTLEDMSGSRMLMQVPQNSLSDIRLGNP
                     VRLLDADVALPARYIITRIHPTGNELRQRLVEAESDQALTTNDLERQCAARLVVAEIS
                     GLILPAETFATAE"
     gene            complement(1040..1699)
                     /locus_tag="PKO06_13555"
     CDS             complement(1040..1699)
                     /locus_tag="PKO06_13555"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNV70721.1"
                     /translation="MPRIHGRNRKQEILDAARELIFTEGATRFTVRRLAERVQITEAA
                     LYRHYMGKEALLLALLDDIFHGWQAGIDEIVGRHESACVRLRALVAFHLEHLFSQHFN
                     PVLLLSDAADPEQEELRQSLCRIAHSLRQAIRALVLEGCKRGELAANLDAELVAQAFL
                     GCVQSSVIEWSLIRSKSGLGARLDRLTEFLIGACHAQRNADGKRLSGVGKKSRNVRTK
                     Q"
     gene            1842..2231
                     /locus_tag="PKO06_13560"
     CDS             1842..2231
                     /locus_tag="PKO06_13560"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV70722.1"
                     /translation="MKSLLLLMQSLADETRLRILMALRTSELCVCQIQALLKLAPSTV
                     SVHMRVLHHAGLVEKRRQGKWVFFRLAKDEATPEGQAAFRLVSRALTACPKIESDRQN
                     LEKLLRRSLPEVCLGTRGAPRQKRDSI"
     gene            2231..>2694
                     /locus_tag="PKO06_13565"
     CDS             2231..>2694
                     /locus_tag="PKO06_13565"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNV70723.1"
                     /translation="MKTRLLFLCTGNSCRSQMAEGWTRHLWPHHFDVWSAGIVKHGLN
                     PHAVRVMQEVGIDISGQVSTTLSDLAGQVFDLVITVCGHAAETCPRFPGSVKLVHVGF
                     DDPPKLALQAHSEEEALGHYRRVREEIRQFIATLPQHPALSDLTGQPDLRSVP"
BASE COUNT          516 a          853 c          718 g          607 t
ORIGIN      
        1 tcttccgctg tcgcaaaggt ctctgccggt agaatcagtc ccgatatttc cgcgacgacc
       61 agtcgggccg cacactgacg ttcaagatcg tttgtggtca gtgcctggtc ggattctgct
      121 tcgaccagcc tctgccgcaa ttcgtttccg gtagggtgaa tgcgagtgat gatgtacctt
      181 gccggaagcg cgacatcagc atcaagcaat cggaccgggt tacccagtcg aatgtccgaa
      241 agcgaattct gcggaacctg catcaacatg cgacttccgc tcatgtcttc aagggtgtag
      301 atggcagtcg aggaagtgac atagtttccc tcttcctggt ggcggccgct gatgactccg
      361 tcgacgggag ctttcagctc ggcatagcgg ttgcgaacct gccagagcga aacggtgtgt
      421 gcggcggcat ctttctgtga ggccacagcc tgcatctggg ctcgtgccgc gtcgatctgc
      481 gcttccagag ccgtgacttt cgccttggcc tccgccaggc ggttctccgt tccctcatac
      541 tgtgctctcg ataatgctcc tctctcatac agcgttcggt cgcgggttgc ctccgcctgc
      601 caataggaca tattggcctt cagtgccggg agctgactgg tgagagaacg gatcgactca
      661 gctgccgccg ccgtctgccg gcccacggct tgagattgtg caccagccat gcgtaactgc
      721 gtttgcagct cgtcgctctc gaggcgagca atcacttctc ccgctttcac cacgtcgcct
      781 tcccgttttc tcagcatcgt cagacgacct gccacttccg gcatcaagcg aatgctcgcc
      841 cgactcttca gcgtcgccag aaccggcacc gtttttctcg cctgtcccgt ctcaatcgtc
      901 ttcacgcgaa tcaagggggc gatcgtgggc atgctcggag cctgcgcctg tcgttcccga
      961 attgccagat accgccacgc cgaggccgcg acgaaaccga caagcaacac ggcaagtatt
     1021 cccagaccca gacttttttt cattgtttgg tcctcacgtt gcgcgacttt ttacccacac
     1081 cagacaacct cttcccatcc gcgtttcgtt gcgcatgaca ggccccaatc aggaactctg
     1141 tcagtcggtc aaggcgagcc cccaaacccg acttgctcct gatcagagac cactcaatca
     1201 cactgctctg gacgcacccg agaaaggctt gtgcgaccag ttcagcgtcg aggttcgccg
     1261 ccaactcacc gcgtttgcaa ccttcgagaa ccagagcgcg aatggcctgt cttaaagaat
     1321 gggcaatgcg gcacaggctc tggcgcagtt cctcctgttc cggatcagcg gcatccgaca
     1381 gcagcagaac cggattgaaa tgctggctga acaggtgttc aagatgaaat gccaccagcg
     1441 ctcgcaggcg cacgcaggcg gattcatgcc gtccgacaat ctcgtcgatg ccagcctgcc
     1501 atccgtgaaa gatgtcatcg agcagtgcca gcagcagtgc ctctttaccc atgtaatgac
     1561 gatacagagc cgcttcggtg atctgcaccc gttcggccag gcgtcgcacg gtgaaccgtg
     1621 tcgctccttc cgtgaagatc agttctcgag ccgcgtccag gatctcctgc ttgcgatttc
     1681 tcccgtggat tcttggcatc gcgcaccttc tatctatagt ttgttatcgt ttacttactt
     1741 tcaatataca tcgacttcgc ccgttggtca accgccctct tgacatcaga caaacgttgc
     1801 ggcattttta tttcgtcatt taacgaattg tgagcaacgt tatgaaatca cttctgctgt
     1861 tgatgcagtc tctggctgat gagacgcggt tacgcattct gatggcgctg cggacgagcg
     1921 aactctgcgt ctgtcagatt caggccctgt tgaagctggc cccctccacc gtctcggtcc
     1981 acatgcgggt tctgcaccac gccgggctgg tagaaaagcg acgccagggc aagtgggtgt
     2041 tttttcgcct ggcgaaagac gaagccacgc cggaaggcca ggcggctttt cgcctggttt
     2101 cacgggctct caccgcctgc cccaaaatcg aaagcgaccg ccagaacctg gagaaactcc
     2161 tgcgccgctc cttgccagaa gtctgtttgg gaacccgagg cgcacctcgt cagaagcgag
     2221 actcaatctg atgaagacgc gtcttctctt cctctgcacc ggcaactcct gccgcagcca
     2281 gatggcggaa ggctggaccc ggcacctctg gccccatcat tttgatgtct ggtcagccgg
     2341 catcgtgaag cacgggctga accctcatgc cgtgcgcgtg atgcaggagg tgggcatcga
     2401 catctccggt caggtctcga caactctcag cgatcttgcc gggcaagtct tcgatctcgt
     2461 catcaccgtc tgcggacacg ccgccgaaac ctgcccgcga tttcccgggt cggtgaagct
     2521 ggttcacgtc ggcttcgacg accctccgaa actggcactt caggcgcact cggaggaaga
     2581 agcactcgga cactatcgcc gggtgcgaga ggagattcgc cagttcattg ccacccttcc
     2641 ccagcatccg gctctctccg atctgaccgg tcaaccggat cttcgatcag ttcc
//
LOCUS       DAORHD010000653         2693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44824_length_2693_cov_3.642532, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000653 DAORHD010000000
VERSION     DAORHD010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44824_length_2693_cov_3.642532"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1696
                     /locus_tag="PKO06_13570"
     CDS             347..1696
                     /locus_tag="PKO06_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV70724.1"
                     /translation="MVELTLRDTSGNMRQVRTSSDRATGLLDGIDIQFKSQAAQMAGI
                     GGLEAGLHFENAEQITFNVGGVFAVTASIAAGDWTLEGITRNINSFIFSAGQTLVGGG
                     DPNGTEFANSLKATVVDGQIRVSWVPPVSVSSANGTIQISGASAATTIGFLNGTYSGF
                     VQGTKNEAETVWGYSRYNDPSTNITAGTVVNLNLYDGNPALAPLAFALDATLGTAQAE
                     LQTADMKLFTEFQASINQALQAAGIEIRVDQVGSTMAFTSLRVGRENRDGQIPLESVV
                     QLTVDDGVAGATAADRWLALRFGIEDGQSRGSGDANFQIHIVDNTPQFQIGADQGQSM
                     KVSFTDMSAEALGVDKLDMTTVAGAQKALAKLNQAIDKVSAERSKLGAFQNRLEYAIN
                     NLRTTNTNLTSAESRLRDVDVSQEMIEFTRNQIINQSGMAMLAQANTLPQGVLQLLQ"
     gene            1784..2158
                     /locus_tag="PKO06_13575"
     CDS             1784..2158
                     /locus_tag="PKO06_13575"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNV70725.1"
                     /translation="MDINSVKPVSPQVPKPASTPSSGVESHGDTAHAPLPGQVNSEVA
                     AQPQDIRERDLKDAVDKMSQTAQIFDRSLKFQIHEETNTMMVAVVDTNTNKVIREIPP
                     KEILDMFARMQDYLGLIFDKKA"
     gene            2182..>2693
                     /locus_tag="PKO06_13580"
     CDS             2182..>2693
                     /locus_tag="PKO06_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HNV70726.1"
                     /translation="MVSGISRNWISGAASGMDTQGLIDKIMEAERSPLNNLEKKRNTI
                     QLQKTMLQEINLKLFEVQTKASDLVFSRTFNSKKVTSSNDKLMSAVADTSAKVGSYQL
                     VVKQIATSTRVSSSYKLASPIELGNNIRSQSQIGGSSLTIGAAGVVTAGNLDVTISGG
                     GTHTLSLGATL"
BASE COUNT          613 a          718 c          814 g          548 t
ORIGIN      
        1 catcgcccag ttctatgatg ccaacggtgt cttcacgctg cagagttcgc agcagctgac
       61 catcatggga aactcgaaga ccacgaccgt gacgattgac ggacagatga ctcttgatcg
      121 tctggccgcg gaactgcaga atgctctgtc gaccagctcc ggtctcggat tgaccaactc
      181 gcgggtcagc atggtcagca cggccattac gggggtctct ggcatgggcg gatacttcca
      241 gatggtttcg ggtgcggttg gtgaagtcgg cgagttcggt attgccggcg accagggagt
      301 cttgaacgct ctcggcgtca gcgttacgcg tgaagcggtg aacagcatgg tagaactgac
      361 tcttcgcgat accagcggga acatgcgtca ggtgcgcacc agttcagatc gagccaccgg
      421 tctgctggat ggtatcgaca ttcagttcaa atcgcaggcg gcgcagatgg cgggtatcgg
      481 cggcctcgaa gccggtctcc acttcgagaa tgccgaacag atcacgttca acgtgggtgg
      541 tgtgtttgcc gtgacggcat cgattgctgc cggcgactgg accctcgaag ggatcacccg
      601 caacatcaac tcgtttatct tctcggccgg gcagactctg gtgggcggtg gtgatccgaa
      661 cggcaccgag ttcgccaact ctctgaaggc gacggtggtg gacggacaga ttcgtgtcag
      721 ctgggttccg cctgtcagcg tgagcagcgc gaacggcacg attcagatca gtggagcatc
      781 ggcagcgacg acgattggtt tcctgaatgg tacctacagc ggctttgttc agggaacgaa
      841 gaacgaggcc gaaacggtgt ggggctactc ccgctacaat gatccttcga cgaatatcac
      901 cgccgggacg gtggtgaatc tgaatctgta tgacgggaac ccggcgctgg cgccactggc
      961 attcgctctc gatgcaacgt taggcacggc gcaagcggag ctgcagacgg ctgacatgaa
     1021 gctgttcaca gagttccagg cgtcgatcaa tcaggctctg caggctgcgg gaattgagat
     1081 tcgtgtcgat caggtcggca gcaccatggc gttcacctca ctccgcgtcg gtcgcgaaaa
     1141 ccgtgatggc cagattcctc tcgaaagtgt tgtgcagctg accgtcgatg atggcgtcgc
     1201 cggggcgacc gcggcagatc gctggctggc cctgcgcttc gggatcgaag acggccagtc
     1261 tcgcggctct ggtgacgcga atttccagat tcatatcgtc gacaatacgc cgcagttcca
     1321 gattggggcc gaccagggtc agtcgatgaa ggtgagtttc accgatatgt cggccgaggc
     1381 tctcggtgtc gataaactgg atatgacaac ggttgccggg gcccagaaag ctcttgccaa
     1441 actgaatcag gcgatcgaca aggtgtcggc cgagcgctcg aagctgggcg cgttccagaa
     1501 tcgtctggaa tacgcgatca acaatctgcg aaccacgaac accaacctga cttccgcaga
     1561 atctcgactc agagatgtcg atgtctctca ggaaatgatc gaattcaccc gaaatcagat
     1621 catcaaccag tcaggaatgg cgatgttggc gcaggcgaat actctgccgc agggagtgtt
     1681 gcagttgctt cagtaaatga ccggggaacc ggggcgaacc ttgcgttcgc cccggaaccc
     1741 atttatgctg tcaatggagt catgagcatt tcggaggttt tttatggata tcaatagcgt
     1801 gaaacccgtg agcccgcaag tgccaaaacc ggcttccact ccttcttccg gtgtggaaag
     1861 ccatggtgat actgctcacg cgccgctccc cggacaagtg aattcagaag ttgccgcgca
     1921 gccgcaggac attcgcgaga gggacctcaa ggatgccgtc gataagatga gccagacggc
     1981 tcagatcttt gatcgctctc tgaagtttca gattcacgaa gagaccaaca ccatgatggt
     2041 ggctgtcgtc gacaccaata ccaacaaggt catccgagag atcccaccga aagagatcct
     2101 cgacatgttt gcgcgtatgc aggactatct gggtctgatt ttcgacaaga aggcctgagg
     2161 tcccggcaaa ggaggtagac tatggtcagc ggtatcagtc gtaactggat cagtggcgct
     2221 gcgtccggca tggataccca gggcctgatc gacaagatca tggaagcgga gcgctctccg
     2281 ctgaacaacc tggagaagaa gcgcaacacc attcagctgc agaagaccat gttgcaggaa
     2341 atcaacctga agctcttcga agtgcagacc aaggcgagcg atctggtctt ttcgcgcacc
     2401 ttcaactcga agaaggtcac ctcctcgaat gacaaactca tgagcgccgt ggcagatacc
     2461 tcggcgaagg ttggatcgta ccagttggtg gtgaagcaga tagccacttc aacgcgagtt
     2521 tcgtctagtt ataaacttgc atcgccgatt gaactgggaa acaatattcg cagccagagt
     2581 cagattggcg gttcgagtct gaccatcggg gccgcgggag ttgtgacggc gggaaacctc
     2641 gatgtgacaa tcagcggcgg agggacgcat acactgagcc tgggagccac gct
//
LOCUS       DAORHD010000654         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_44940_length_2689_cov_3.245254, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000654 DAORHD010000000
VERSION     DAORHD010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44940_length_2689_cov_3.245254"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKO06_13585"
     CDS             <1..598
                     /locus_tag="PKO06_13585"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV70727.1"
                     /translation="QLAFAVGMLVLGVGLLVSRFVPSTTPNQTPVPASAWQIQAEAPK
                     ARIDGQPVVRGPLHARDSISFSVDGDGEFMLSWHQQSTIRLMQARGRLHPTAIELDTG
                     SVQISSNAPTKASVLEVRTPFGTVREIGTIYVVEVKADEASVRVEEGKVHLECGQATR
                     IVAAGQMATMNRQGIVLPSQHVSPPTTPPVDRVPTPGE"
     gene            610..1185
                     /locus_tag="PKO06_13590"
     CDS             610..1185
                     /locus_tag="PKO06_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV70728.1"
                     /translation="MKPPEPSISDAECVAASRRGDQKAFGQLIQRYQALVYRFLFHFT
                     GNASDAEDLTQETFLNLYRKLDLHDPKRKFSAWLLTMARNLAVSQHRRRTPTPLAPEI
                     VAEAIRDVLPSPESSAVWNETANELHLTIQKLPDDLREAVIFRYLLDLQINEIAVLLE
                     LPEGTVKSRVFKARQELRDALARLERTPTRA"
     gene            complement(1591..2337)
                     /locus_tag="PKO06_13595"
     CDS             complement(1591..2337)
                     /locus_tag="PKO06_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70729.1"
                     /translation="MKIRKGWMAWVLVLALAFQPLFLEAMAQAEESSAAQGKMNEANT
                     KLDEAQNKVNSNSTAQQKAGAQLEQANQQQQAGQAQAAAGESEQKSGQNSTLDKVMQM
                     KAKIQKIMVKVGEILEKVGQILQTVGTMLIAIGTAMSAFFGSGAALISIGKQLYSIGG
                     MIKNIGAFIKKLGTDAKALDQSFGDIVKGVFNAAKEGYANGQKEADAKAQEIQGQMNK
                     AADSVKSTTSSGGDSSSDQPVDRAPTADGE"
     gene            complement(2420..>2689)
                     /locus_tag="PKO06_13600"
     CDS             complement(2420..>2689)
                     /locus_tag="PKO06_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70730.1"
                     /translation="AAAKTLNRLDAGQVKVFQGFENVEFKLYVHECNPQVPMLWVKIV
                     VNESGNIYGNNLKADRALELTTTISSAFVTSNLENLSWNYETAHQ"
BASE COUNT          548 a          830 c          667 g          644 t
ORIGIN      
        1 tcaactggca ttcgccgtgg gcatgctcgt tctcggtgtt ggactgctcg tttcgcggtt
       61 tgtcccttcg acaactccga atcagactcc cgttcctgct tccgcctggc agattcaagc
      121 cgaggcaccg aaagcgcgga tcgacggaca acccgtcgtg cgtggccctc ttcacgcgcg
      181 cgattccatc tcgttttccg tcgatggtga tggcgaattc atgctgagct ggcaccagca
      241 gtcgacaatt cgcctgatgc aggcacgcgg aaggctgcat ccgacggcaa ttgagctcga
      301 cactggatca gtgcagatca gttcgaacgc cccgaccaaa gcctcggttc tcgaggtcag
      361 aactcccttt ggaacagtgc gcgaaattgg tacgatctat gttgtcgaag tgaaggctga
      421 tgaagcctct gtccgggtcg aagaagggaa agtccatctc gaatgcggcc aggcgactcg
      481 catcgtcgct gccggacaga tggctacgat gaatcgccaa ggtattgttc ttccgtctca
      541 acacgtctca ccaccaacaa ccccaccggt cgaccgggtt ccaactccag gagagtagca
      601 gtcattgttt tgaagccccc tgagccatca atttcagatg ctgaatgcgt tgcggccagt
      661 cgacgcggtg atcagaaggc ctttggtcag ctgatacaac gttatcaggc tctggtgtac
      721 cggtttcttt ttcatttcac cgggaatgcc agcgatgcgg aagacctgac gcaggaaacc
      781 tttctcaacc tgtatcgcaa actcgatctg cacgatccca aacgaaaatt ctcggcgtgg
      841 ttgctgacca tggcccgcaa tcttgcggtt tctcagcatc gccgacgcac cccaaccccg
      901 ctcgcccctg aaatcgtggc cgaagccata cgcgatgtgc tcccgagccc cgaatcaagc
      961 gcagtctgga acgaaaccgc caacgaactg catctgacca ttcagaaact tcctgacgat
     1021 ctgcgagagg ccgtcatctt tcgctatctc ctcgatctgc agatcaatga aatcgccgtg
     1081 ctgctcgaac tcccagaagg aaccgtgaaa tctcgcgtgt tcaaggcccg ccaggagctg
     1141 agagacgctc tcgcccgcct cgagcgcact ccaacacgcg cgtaagactc gcacacggtg
     1201 tagtttcaac tccgcagcga cgccctttcc tgaaatcacg ggattacacg taacagagct
     1261 tgcgctcacc ctcgtcaacg gtcttcgaac tcctgttcga cgattcgttc agggcgaccc
     1321 aggtgccaac acatcgcttc gagggatgcc tcggatttct ctcgagaggt tcagctctca
     1381 cgcagctcct gacatcacac tcggcgacgt ccatcccgat gcccgatgct tcacgagccg
     1441 tggctgaagc tcgtcgtttc gtgtatcgcc tgaccccatg cgtatctcac ctgaagttcg
     1501 acagaaccag agacccgaga ataccgcaaa attgcccaaa aaaagaaaca ccggcccgaa
     1561 ggccggtgct ctctgatgcg aaccggttac ttactctcca tcagcggtcg gagcgcggtc
     1621 gacaggctgg tccgaactgc tgtcgccacc cgagctggtc gtgctcttga ctgagtcggc
     1681 ggctttgttc atctggccct gaatttcctg agccttggcg tcggcttcct tctggccatt
     1741 ggcatagcct tccttcgcgg cattgaaaac gcccttcacg atatcaccaa agctttgatc
     1801 aagcgctttt gcgtctgtcc caagcttctt gatgaaggca ccgatgttct tgatcatgcc
     1861 accgattgaa tacagctgct ttccgataga gatcaacgca gcgcctgaac cgaagaacgc
     1921 ggacatcgcg gttccgatgg caatcagcat cgtgccaaca gtctgtagaa tctggccgac
     1981 tttttccaga atctcaccga ctttcaccat gatcttctgg atcttggcct tcatctgcat
     2041 gaccttgtcg agggtgctgt tctgtccgct cttctgttca gactcaccgg cggcggcctg
     2101 agcctgtcca gcctgctgct gctgattggc ctgctccagc tgggcaccgg ccttctgctg
     2161 cgcggtgctg ttgctgttca ccttgttctg cgcctcatcc agcttcgtgt tcgcttcgtt
     2221 cattttcccc tgggcggctg acgactcttc agcctgagcc attgcctcaa ggaacagcgg
     2281 ctgaaatgcc agcgccaaca ccagaaccca cgccatccaa ccttttcgga tcttcatatc
     2341 tctacctcct ctgaaatttg tttcccgaat gggtttcact ttcattacgc cacaggttcg
     2401 caaacggttc aaactttttt tactgatgtg cggtttcgta gttccagctc aggttttcca
     2461 ggtttgaggt cacgaacgca ctcgagatgg ttgtcgtcaa ctcaagcgct cgatctgcct
     2521 tcaggttgtt cccatagatg ttcccggatt cattcacgac gatcttcacc cagagcatgg
     2581 gcacctgggg attgcattcg tgcacgtaca gtttgaactc cacgttttcg aatccctgaa
     2641 agactttgac ctgaccggcg tcaagtcgat tcaaggtctt cgccgccgc
//
LOCUS       DAORHD010000655         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45023_length_2686_cov_3.489928, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000655 DAORHD010000000
VERSION     DAORHD010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45023_length_2686_cov_3.489928"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKO06_13605"
     CDS             complement(<1..202)
                     /locus_tag="PKO06_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70731.1"
                     /translation="MIRSRLLKILRSGPLFWGLCFCLLTFMAQATPVTRPDGERPEAR
                     KSEPKKTEEEFRREKAQLLIKKG"
     gene            complement(211..402)
                     /locus_tag="PKO06_13610"
     CDS             complement(211..402)
                     /locus_tag="PKO06_13610"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HNV70732.1"
                     /translation="MRAVVESVEGKMVRCLMENGDILTVGRQQLPDDVKEGDVIKLSF
                     TLDTESTKRQKELMAGLTS"
     gene            complement(470..826)
                     /locus_tag="PKO06_13615"
     CDS             complement(470..826)
                     /locus_tag="PKO06_13615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70733.1"
                     /translation="MEKVTRYTLAQIADEIFSEIRATMVRMRSDLGVDISLLGGFAQE
                     TIAMRLADSSFMSIKVFPHRNFQSQNVFRVEVYKTIPGDMRGNRITFVEISQEGTDPE
                     DIRKFTEGVLSRSGII"
     gene            complement(814..2529)
                     /gene="thrS"
                     /locus_tag="PKO06_13620"
     CDS             complement(814..2529)
                     /gene="thrS"
                     /locus_tag="PKO06_13620"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV70734.1"
                     /translation="MKSTDAADVYRHSMAHIMADAVLKLFPDAQFGIGPVIENGFYYD
                     FLLPRALTPEDLNALEKEMKEIINRKEPIEYKLMSKPEAIELFTRLNQPFKIELIHEI
                     PDEKVSVYSQGGFVDLCRGPHLPNTGFVKHFKLLSIAGAYWRGDEKRPMLQRLYATAF
                     PSKEELDEYLHNLEEAKKRDHRKLGKDLDLFSLHEEVGPGLVFWHPNGARIRHAIESF
                     WKDQHYSNGYELIYTPHMGRSWLWETSGHLDFYKDGMYSPMQIDEFDYYIKPMNCPFH
                     IMIYKSQNRSYRDLPLRWAELGTVYRYEKSGVLHGLLRVRGFTQDDAHIICTPEQIES
                     EILEVLRFSLSLWKAFGFEKIKAYLATRPEKAVGDPARWDQAIGALRRAVEAEKLDCE
                     IDEGGGAFYGPKIDLKVRDAIGREWQMTTIQFDFNLPERFDMTYTGADGNKHRPYMVH
                     RALLGSLERFFGVLVEHYGGGMPLWLAPVQAAILPISDKHLEYARAVEARLRKSMIRT
                     SLDDRGDKIGAKIRNAQLQKVPFMIIIGEEEASSGKITVRKRSGENLTGLDLETFVQE
                     QEWKK"
BASE COUNT          569 a          783 c          700 g          634 t
ORIGIN      
        1 gacccttttt gatcaacaac tgcgcttttt cgcgtcgaaa ctcttcttcg gtcttcttcg
       61 gctcggactt tcgcgcttcc ggtctctcgc catcaggccg agtaaccggg gttgcctgcg
      121 ccataaaagt cagcaggcaa aaacacaggc cccagaacag ggggcctgag cgaaggatct
      181 tcagcaaccg cgagcggatc attccgcctc tcaggaggtc agacctgcca tcagttcttt
      241 ctgccgcttc gtgctttccg tatcgagagt gaacgagagc ttgatgacat ctccttcttt
      301 cacgtcatcg ggaagctgct gacggccaac cgtcaggatg tcaccatttt ccatgaggca
      361 gcgaaccatt tttccttcaa ccgattcaac aacagcgcgc atacaggctc ctcttgtttg
      421 ttttcattca acgccccatc cgggcgtgac gtgcgggttt cgatgtgtcc tagatgattc
      481 ctgagcggga aagcacaccc tcggtgaact tgcggatatc ttccggatcc gtgccttcct
      541 gagagatttc cacgaaggta atgcggttac cgcgcatatc tccggggatg gttttgtaca
      601 cttcgacacg aaacacattc tggctctgaa aattccgatg cggaaagact ttgatcgaca
      661 tgaatgacga gtccgcaaga cgcatggcga tggtctcctg cgcgaacccg cccagcagcg
      721 agatatcaac cccaaggtca ctgcgcatgc gcaccatcgt cgcccggatc tcggagaaga
      781 tctcgtcggc gatctgtgcc agcgtgtacc gggtcacttt ttccattcct gctcctgaac
      841 gaaggtttcg agatcaaggc cggtcagatt ctcgcccgag cgcttgcgca cggtgatctt
      901 tccgctcgag gcttcttctt cgccaatgat gatcatgaac ggcactttct gcaactgggc
      961 gttgcgaatc ttcgcgccga tcttgtcccc gcgatcatcg agcgaggtgc gaatcatcga
     1021 ctttcgcaat ctcgcctcga ccgcgcgagc gtactcaaga tgcttgtcgg aaatcggcag
     1081 aatcgccgcc tgcaccggag cgagccacag cggcatcccg ccaccatagt gctcgaccaa
     1141 aaccccgaaa aatcgttcga gagacccgag aagcgcgcgg tgcaccatat acggtcgatg
     1201 cttgttgcca tcagcaccgg tgtaggtcat gtcgaaacgt tccgggagat tgaagtcgaa
     1261 ctgaatcgtc gtcatctgcc attcgcgtcc gatcgcgtct cggactttca gatcgatctt
     1321 cgggccgtag aacgctccac cgccttcatc gatctcacaa tccagctttt cagcttcaac
     1381 ggctcggcgc agagctccga tggcttgatc ccagcgggca gggtcgccaa cggccttttc
     1441 cggacgagtg gccagatagg ctttgatttt ctcgaatccg aaggctttcc acagcgaaag
     1501 gctgaagcgc agcacttcga gaatctccga ctcgatctgc tcaggggtgc agatgatgtg
     1561 cgcatcatcc tgagtaaacc cgcgcactcg cagcagacca tgcagcactc cacttttctc
     1621 ataccgatac acggtgccca gctcagccca gcgcagcggc agatcacgat acgagcgatt
     1681 ctgtgacttg tagatcatga tgtggaaagg gcagttcatg ggcttgatgt agtaatcaaa
     1741 ctcatcgatc tgcatcgggc tgtacatgcc gtctttgtag aaatcaaggt gaccactggt
     1801 ctcccagagc cagctccgcc ccatgtgtgg cgtgtagatc agctcgtacc cattgctgta
     1861 atgctggtct ttccagaagc tttcgatcgc gtggcgtatg cgtgcgccgt tcgggtgcca
     1921 gaacaccaag ccaggcccca cttcttcgtg aagcgagaag agatcgagat ctttgcccag
     1981 tttgcggtga tcgcgcttct tggcttcttc gaggttgtgc agatactcgt cgagctcttc
     2041 tttactcgga aaagccgtgg catacaagcg ctgcagcatc ggccgcttct cgtcaccccg
     2101 ccagtacgcc ccggcaatcg acaacaactt gaaatgcttg acgaagccgg tgtttggaag
     2161 atgcggacca cgacacagat cgacgaaccc gccctgcgaa tacaccgaca ccttttcatc
     2221 aggaatctcg tgaatcaatt cgatcttgaa cggctgattg agcctggtaa aaagctcgat
     2281 ggcttcaggc ttcgacatca gtttgtactc aatcggctct ttgcgattga tgatctcttt
     2341 catttctttt tcgagcgcat tgagatcttc gggagtcagg gctcgaggca acaggaaatc
     2401 gtagtagaac ccattttcga tgaccgggcc gatgccaaac tgtgcgtcag gaaacagctt
     2461 caacacggca tctgccataa tgtgggccat gctgtggcga tacacatcgg ccgcatcggt
     2521 cgacttcatg gtaatgactt cgaaagcccc cgattcatcg atcgggagcc ggacatcgcg
     2581 cacggttccg ttgatcttca cgccgatggc tgctgcagcc agcttgggcc caatgctgtc
     2641 agcaacggct cgaccggtgg ctccgtgcgg gtgttctttt tttgat
//
LOCUS       DAORHD010000656         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45037_length_2686_cov_2.369061, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000656 DAORHD010000000
VERSION     DAORHD010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45037_length_2686_cov_2.369061"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKO06_13625"
     CDS             <1..410
                     /locus_tag="PKO06_13625"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNV70735.1"
                     /translation="AKLTGRYRLANRDLELQIIDTWLGDTRASVKNLSGGEKFLASLA
                     LALGLAQVVGQKHRMETLFIDEGFGALDADTLETAVNALCSLQQQGKLIGIISHVDRV
                     KERLPVRLELEPAGIGFSTLTGPGCQKLPTPAA"
     gene            complement(542..2590)
                     /locus_tag="PKO06_13630"
     CDS             complement(542..2590)
                     /locus_tag="PKO06_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV70736.1"
                     /translation="MIDRSGSMDGQPMTRTLSAVSEVVELLTRHDLATIVAFDGQVDV
                     IQELGPVTNKLEIHQKVQQLYARGGTNLSAGWLKALQILSEKTDEECFKRVILLTDGQ
                     VGAGVQELEKLGTIASTFLQKGISTSTFGLGRGFNENVLKLIAEKGNGNFYYIEGPED
                     VSSAFQFEFGELNAIVGQNLEVQISCGKGIEILETLGHGSAKSSANTCTVSLGDVSEG
                     LEKNLVLRLRVPREIAHGSQRLFEFSTSYQKASGDYATVRSTSEFATEFRSGDVRRYL
                     DHEVLDDVMIERTARLKIQVYQDVQAREVGKALTMLNERINLLGERVLTTDVVKKEEL
                     EAEIAALETIKQAIEEKSADQGKVIQQQVEDFQKKKGSYLKTRAERKYILNSVLAAQA
                     QDEFQELMKHLGDKLNDLGQEGEFRQNCLICMQELVANALEHGCQQVQKGEVQVRGIF
                     RKGSAMITVSDPGAGFDWEEKVKTARANIELAEKARNDQSGTPQQTAQSLKGTSTRGR
                     GIQLLLTLADEVTYTCAGSEVMVKLSNKVSEGVISFDNTTVSSSSPFASSFSVKVRNH
                     VINQTPMVELEPSECVSTFVCQQAKQAVKQVMAKGAVHLILNLKNVPFTDSAGLGFYC
                     GALKHCVEAGGSLSLIVACDNMKRVFNMIQILQWIPCFATLDDLRSSLENPDAGKLSA
                     "
BASE COUNT          531 a          819 c          729 g          607 t
ORIGIN      
        1 tggccaagct gaccggccga taccgactgg cgaatcgcga cctcgaactg cagatcatcg
       61 acacctggct cggcgacacg cgcgcgtcgg tgaagaacct ttccggcggt gagaagtttc
      121 tggccagtct ggcgctggcg ctgggtctgg ctcaggttgt cggtcagaaa caccgcatgg
      181 aaaccctctt catcgacgaa ggcttcggcg cgctcgatgc cgacaccctt gaaaccgccg
      241 tcaacgccct ctgcagtctt cagcaacagg gcaaactgat cggcatcatc tcgcacgtcg
      301 accgcgtcaa agaacgactg cccgtccgcc tcgaactcga acccgccggc atcggcttca
      361 gcaccctcac cggccccggc tgtcaaaaac tccccacccc cgccgcgtag aacaagcgaa
      421 gctcggcgtg gaaaacgccg gttttaagcc gctactgact tggccttcgc caggcgagaa
      481 aaagaaagcc accccgacgg gacggggtgg caaaaaacaa aggaggcttg cgatcgtttc
      541 gttacgcgct taacttccct gcatcagggt tttccagact ggaccgcagg tcatcgaggg
      601 tggcgaagca agggatccac tgaagaatct gaatcatgtt gaataccctc ttcatgttat
      661 cgcaggctac gatcagcgag agactgccgc ctgcttccac acagtgtttc aaggccccgc
      721 agtagaatcc gaggccagcg ctgtccgtga acgggacgtt cttcagattg agtatcagat
      781 gcacagcgcc cttggccata acctgcttga ctgcctgttt cgcctgctga cagacaaatg
      841 tgctcacgca ttcagatggc tcgagctcga ccatcggcgt ctggttgatg acgtgattcc
      901 gcaccttgac cgagaacgac gaagcaaaag gagagctcga tgagaccgtc gtgttgtcga
      961 acgagatgac cccttcagag accttgttcg agagcttgac catgacttcg cttcccgcac
     1021 acgtgtaggt gacttcatcc gccagcgtca gtaacagttg gatgccacga ccgcgtgtgc
     1081 tcgtgccttt cagactttga gccgtctgct ggggggtgcc gctctggtcg ttcctggcct
     1141 tctcagccag ctcgatgttg gcccgtgctg tcttgacctt ttcctcccag tcgaagcccg
     1201 cgcctgggtc actgacggtg atcatggccg aacccttgcg aaagatgccg cgcacctgca
     1261 cttcgccctt ctgtacctgt tgacagccgt gctcgagggc attggcaacc agttcctgca
     1321 tgcagatcag gcagttctgt cgaaactcgc cttcctgccc gaggtcattc agtttgtcgc
     1381 cgaggtgttt catcagctcc tggaactcgt cttgggcttg agccgccaga acggaattga
     1441 gaatgtattt gcgctcggcc cgtgttttca ggtaactgcc cttcttcttc tgaaagtctt
     1501 cgacctgttg ctgaatgacc ttgccctgat cagccgactt ctcttcgatg gcctgtttga
     1561 tcgtctcgag ggccgcgatc tccgcttcca gctcttcctt tttcacgacg tccgttgtca
     1621 gcacccgttc gcccagaaga ttgatgcgct cattcagcat ggtcagggct tttccgacct
     1681 cgcgagcctg gacatcctga tagacctgaa tcttcaggcg cgccgtgcgt tcgatcatga
     1741 cgtcatcgag cacttcgtga tcaagataac gtcgcacatc ccctgagcgg aactccgtag
     1801 cgaactccga agtggagcgt accgtcgcgt agtcgcccga cgccttctga tacgaggtcg
     1861 agaactcgaa cagccgctgg cttccgtgcg cgatttcgcg aggcactctg agtcgcagaa
     1921 ccaggttctt ttccaggcct tccgacacat cgccgagcga gacggtgcag gtgttggccg
     1981 acgatttggc actgccgtgg ccaagcgtct cgagaatttc gatgcccttt ccgcaggaga
     2041 tctgcacttc gaggttctgg ccgacaatcg cgttcagttc cccgaactcg aactggaaag
     2101 ccgagctgac gtcctcgggg ccttcgatgt agtagaagtt gccgtttccc ttctcggcga
     2161 tcagcttcag gacattctca ttgaaaccgc gcccgaggcc gaacgtcgag gtactgattc
     2221 ccttctgaag gaatgtggag gcgattgttc ccagcttttc gagttcctga acgcccgcgc
     2281 caacctgacc gtcggtcagc agaatgacac gtttgaagca ttcttcgtcc gtcttttccg
     2341 acagaatctg cagggctttg agccacccgg ccgacaggtt ggtgccgccg cgcgcataga
     2401 gttgctgaac cttctgatga atctcgagct tgttggtgac agggccgagt tcctgaatca
     2461 catcgacctg accatcgaaa gcgacgattg ttgccagatc gtggcgggtc agcagttcca
     2521 ccacttcgga aacagcgctc agggttctgg tcatcggttg tccgtccatc gacccactgc
     2581 gatcgatcac cagggcgaga gcaatcttct tccgccctga gtatcgctat ctgcctgaag
     2641 attccgagtc tcagtttcac gtgatcacgt cgctgaaggc agaaga
//
LOCUS       DAORHD010000657         2683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45109_length_2683_cov_2.438737, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000657 DAORHD010000000
VERSION     DAORHD010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45109_length_2683_cov_2.438737"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2068)
                     /gene="dnaE"
                     /locus_tag="PKO06_13635"
     CDS             complement(<1..2068)
                     /gene="dnaE"
                     /locus_tag="PKO06_13635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV70737.1"
                     /translation="MYHSNFVHLHVHSHYSLLDGAASVKSIVEAARRYRMPAIALTDH
                     GNMFGAIDFYGTAMKRGVKPILGFESYVVEGSRHARGEAKENKYYHLTLLARDMIGYK
                     NLIKLTSFGYTEGFYYKPRIDWEVLEKHNQGLIVLSGCLQGRIPQLLLQGKPDEADAT
                     ARRYQDIFGRENFYIEIMNHAIEDEMRVLGPLIELSRRLEIPLVATNDSHYASANDWE
                     AQDALLCLATNKLLNDPTRWRFESRDLYVKSPQQMAELFREVPEALTNTMRVADRCNV
                     ILPMGKSILPHFELPDGLDSNAYLDKLCREALPGRYGDPIPPEIINRLEYELGVIKRM
                     GFPDYFLIVWDFIAEARRIGVPVGPGRGSAAGSIVAYLLGITDIDPLKYGLLFERFLN
                     PDRISMPDIDIDFSDEGRERVIEYVVRKYGREKVSQVITFNTMLAKMAIRDIGRVMGI
                     PIPEVDKLAKLVPEKPGTHLKKVLSEDGELKTVNESGTEQEKQLLRIAASVDGMVRHT
                     GVHAAGVVISREPIMDIVPLYKDPKSGAIVTQYEKNAIEKIGLLKMDFLGLKTLSVIQ
                     RALDYIKEGHGVELNLGKLTVDDQATYDLLCRGLTMGVFQLESSGMRSLITRLRPTVF
                     EDIIALLAMYRPGPLGSGMVDDFVECKHGRKKIVYPHPDLEPVLKDTYGVFLYQEQCM
                     QTANILA"
     gene            complement(2164..2520)
                     /locus_tag="PKO06_13640"
     CDS             complement(2164..2520)
                     /locus_tag="PKO06_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70738.1"
                     /translation="MNKVLLSVLCLSLLVMVGCGGGQPAPQTPAAPAAAPAAAPANTT
                     AAPAAATPAPTAAPAPAADPAAAPAAAPAAAPATPAPAAAPAADAPAAAPAAAPATPA
                     PAAAPAADAPAPAAGS"
BASE COUNT          541 a          725 c          872 g          545 t
ORIGIN      
        1 cggccagaat gttggcggtc tgcatgcatt gttcctgata caggaacaca ccgtaggtgt
       61 ccttgagcac gggttcgagg tcagggtgcg ggtagacgat cttcttgcgg ccgtgtttgc
      121 attcgacgaa gtcatcgacc atccccgacc cgagcggacc ggggcggtac atggccagaa
      181 gcgcgatgat gtcttcgaag acggtcggac gcaggcgggt gatgagcgag cgcatgcccg
      241 acgattcaag ctggaacacg cccatcgtga ggcccctgca gagcaggtcg tacgtcgcct
      301 gatcatcgac cgtgagcttg ccgaggttca gttcgacacc gtgaccttcc ttgatgtagt
      361 caagtgcgcg ttgaatgacc gagagggttt tgagaccgag aaagtccatt ttcagcaggc
      421 cgatcttctc gatcgcgttc ttttcatact gcgtgacgat agctccggac ttcggatcct
      481 tgtagagcgg cacaatatcc atgatcggtt cgcgggaaat gacgacgccg gccgcgtgca
      541 cgcccgtatg ccgtaccatg ccgtcgaccg aggcggcaat gcgcagcagt tgcttttcct
      601 gctcggtgcc actctcattc acggttttga gttcgccgtc ttcggacagc acctttttga
      661 gatgcgtgcc aggcttctca ggaaccagct tggcgagttt gtcgacctcg ggaatgggga
      721 tgcccatgac ccggccgatg tcgcgaatcg ccattttggc gagcatggtg ttgaaggtga
      781 tgacctgcga gaccttctcg cggccgtatt ttcgtaccac atactcgatg acgcgttcgc
      841 ggccttcgtc ggaaaaatcg atatcgatgt cgggcatgct gatacgatcg gggttgagaa
      901 accgttcgaa caacagaccg tatttcagcg ggtcgatgtc cgtgatacca agcaggtagg
      961 cgacgatact gccggcggcc gatcctcgtc ccgggccgac cggtaccccg atacggcggg
     1021 cttcggcgat gaaatcccag acgatcagaa agtagtcggg aaagcccatg cgcttgatga
     1081 cgcccagctc atactcgagg cgattgatga tctcgggggg aatcggatcg ccgtatcggc
     1141 cggggagcgc ctcacgacag agtttgtcca ggtaggcatt cgagtcgagc ccgtcaggaa
     1201 gctcgaaatg aggcagaatc gatttgccca tcggcagaat gacgttacag cgatcggcca
     1261 cgcgcatcgt gttggtgaga gcctcgggaa cctcgcggaa cagctcggcc atctgctgag
     1321 gcgacttcac atagaggtcg cgcgactcga agcgccagcg ggtcgggtcg ttgagcagtt
     1381 tgttggttgc cagacaaagc agggcgtcct gagcttccca gtcattggcc gaggcgtaat
     1441 ggctgtcgtt ggtggccacg agcggaatct cgagtcgacg cgacagttcg atgagcggcc
     1501 ccagaactcg catttcatct tcgatcgcgt ggttcatgat ctcgatatag aagttctcgc
     1561 gaccgaatat gtcctggtag cgacgggctg ttgcgtcggc ctcgtcaggt tttccttgta
     1621 gcagaagttg aggaatacgc ccctgcagac aaccgctgag tacaatcagg ccctggttgt
     1681 gtttctcgag gacttcccag tcgatgcgag gcttgtagta aaacccttcg gtgtacccga
     1741 acgacgtgag cttgatcagg ttcttgtacc cgatcatatc gcgggcgaga agcgtcagat
     1801 ggtaatactt gttttccttg gcttcgccgc gggcatgccg cgagccttcg accacatagg
     1861 attcaaagcc gagaatcggc ttgacgccac gtttcatggc cgtgccgtag aagtcgatgg
     1921 ccccgaacat gttaccgtgg tcagtgagag cgattgcagg catgcggtac cgccgcgctg
     1981 cctcgacgat ggattttaca ctggcagcac cgtcaagcag gctgtagtgg ctgtgtacat
     2041 gcagatgtac aaaattcgaa tggtacatgc gcccgaaagt atagcatgaa actttctgaa
     2101 aagcagaaac ggggtaccgg gccgttgata cggctcggca ccccgtctgg gtgcagggag
     2161 aacttagctg ccggcggcgg gagccggagc gtcagcggcg ggcgcggcgg caggagccgg
     2221 ggtcgccgga gcggcggccg gggcggcggc aggagcgtca gcagcgggag cggcggcggg
     2281 agccggagtt gccggagcgg cggcaggagc agcggccgga gcggcagcgg gatcagcagc
     2341 cggggcggga gcagcggttg gagccggagt ggcagcagcc ggagcggcag tggtattcgc
     2401 cggggcagca gccggagcag cggcgggcgc ggccggagtc tgcggagcgg gctgaccgcc
     2461 gccacaaccg accatgacca acaaactgag acacaaaacg ctgagtagaa ccttattcat
     2521 gaaggtaccc ccttttggaa ttgcgggcga atatagcaga attgccgcaa ttgttcaatt
     2581 attctttttc gcgggcggag catgcagcac cagcgcttgt gaagcgagct ccggtgtcac
     2641 cttcttctgc aatctctcag ggagtttcgg cgaccacttg atg
//
LOCUS       DAORHD010000658         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45134_length_2682_cov_2.839360, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000658 DAORHD010000000
VERSION     DAORHD010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45134_length_2682_cov_2.839360"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(324..1472)
                     /locus_tag="PKO06_13645"
     CDS             complement(324..1472)
                     /locus_tag="PKO06_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNV70739.1"
                     /translation="MVAALIETPGVKNGNGAETRKRKKRANGEGYIFRCGRRWGGQVW
                     AFCPNKGKKRLMSFTGSTRAEVAEKIAVARQVHDYMAAKTVTVAEWFELWMTRYMIDI
                     RTSTRDTYRRTIHGHIVPHLGAQNLNRLTPDLVSTALSRMGETLCPKSLQLVHAILSK
                     GFRQAVVSGYAWRNVMQAVSKPRTEAKDHQVFTREELVRFLEAAKTSRFDLFFLLVAS
                     CGLRRGEGLGVSWSDISFERGELTIRRNIVKTSQGIEVNPPKTKASRRTIHLPPYVMD
                     ELKKVNARYRRGFVTKTASGEPVCPDRVSAEFKKLCRLFDLPNLKLHELRHTHASLML
                     SSGVPVSDVQRRLGHNDSRTTLDTYSHAIPGDGDRAAEIMDRIMRGEE"
     gene            complement(1587..1931)
                     /locus_tag="PKO06_13650"
     CDS             complement(1587..1931)
                     /locus_tag="PKO06_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70740.1"
                     /translation="MRLACVLLWSIPACCGLAVLSDEHPAVTPAGGGACQGVPLPDLT
                     TAVQTAVKLAFLFPRVEKQMSEGLDRVNQAEAAGDERALWWAVFNLELLCLRVWRDHQ
                     PGVTRGLNVSRI"
     gene            complement(2119..>2682)
                     /locus_tag="PKO06_13655"
     CDS             complement(2119..>2682)
                     /locus_tag="PKO06_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70741.1"
                     /translation="ALTVLLGYFAIGAPRIVPGNTASFEDWSNSVRQCVAWLGAAGIY
                     ETIDPARVIEEAMENDNRLGVLARFLGAWYEALGPRAVTVAEVLERANSSFELMNAIR
                     EKHPEEAGRALHIGYWLKQQKGRVEGGYRLEAAGKDRLNVALWRVIRISRVNEAECRG
                     WNADADGVTPEMQGMQGMFPVLREENA"
BASE COUNT          573 a          882 c          640 g          587 t
ORIGIN      
        1 ccccacttgc gattttggcc gtgtaagaat ttgtctcccc tggtcggtcc cgttctataa
       61 ctgctaggtt tttgacaccc cctcccccct gacgcccttc tgatgccggg ttttcagcac
      121 ttgggcggga ataccacacc atcggacacc ttgcccggca aggctgtggc gctctgacca
      181 actctcgaaa ggattccgag catgcgccag gctgtggcgc accggcaaac cttcgaggaa
      241 gttccgagag accggcaagg gtgtgacgca ccggcaaacc ttcgagaatg atccgggcca
      301 ccggcaaggc tgtggcgtgc cggtcattct tcgcccctca tgatccggtc cattatctca
      361 gcggccctgt ctccgtcgcc tggtatcgcg tgagagtaag tatcaagcgt tgttcgactg
      421 tcattgtgac ctaaccgccg ctgaacatct gataccggaa caccgcttga gagcatcagc
      481 gatgcgtgag tatgcctgag ttcgtgcagc ttcaggttag ggaggtcgaa caaccggcac
      541 agttttttga actctgcgct cacccgatca gggcataccg gctcacctga tgcggtcttc
      601 gtgacgaacc cccggcgata ccttgcgttg accttcttca attcatccat cacatacggc
      661 ggcaagtgaa tggttcgcct agatgctttg gtcttcggcg ggttcacttc gataccctga
      721 gaggtcttta caatgttccg ccgtatggtc agttcgccgc gctcgaagct gatatcagac
      781 catgacaccc ccaagccttc accccgtctc aacccgcatg atgccaccag aagaaagaac
      841 aggtcgaagc gtgaagtttt cgccgcttcc aagaaacgta caagttcttc tctcgtgaat
      901 acctggtggt ctttcgcttc tgttcgaggc tttgaaactg cctgcatgac attccgccag
      961 gcataaccag acacgacggc ctgacgaaaa ccctttgata gaatcgcatg caccagttgc
     1021 agagacttcg ggcacagcgt ctcacccatt cgtgatagag ccgtcgagac aagatcaggg
     1081 gtcaaccggt tcaggttttg tgcccccagg tgcggcacga tatgcccgtg aatcgttcgc
     1141 cggtaggtgt ccctggtcga ggttcgtata tcgatcatgt aacgggtcat ccacaactca
     1201 aaccattcag caaccgttac cgtcttcgct gccatgtaat catgcacttg ccgggctact
     1261 gctatttttt ctgccacttc tgcgcgggtc gatccggtga aagacatgag ccgctttttt
     1321 cccttgttcg ggcagaacgc ccaaacctgc ccgccccatc gtctgccaca gcgaaagata
     1381 tacccctctc cgttcgccct cttttttcgt tttctggttt ccgccccgtt tccgtttttt
     1441 accccgggcg tttcgatcaa tgcagcaacc acagcgaaac ctcctatttc attcccaaat
     1501 cacccgcaac cccgcaccaa cccaccactc ccaaaccacc cccaaacttg cccggatttc
     1561 aaatccgctc aaccttcact cggatttcaa atccgactca cgtttaaacc gcgagtgaca
     1621 cccggctgat ggtcacgcca gacccgcaga cacaacaact caaggttgaa caccgcccac
     1681 cacaaggccc gctcatcgcc cgccgcctct gcctggttca cccggtctaa cccctcgctc
     1741 atctgcttct caaccctcgg gaacaggaaa gccaacttca ccgccgtctg tactgccgtc
     1801 gtcagatcag gcaacggcac gccctgacac gcgccaccac ccgcaggagt taccgccggg
     1861 tgttcgtcac tcaacacggc cagaccgcaa catgcaggga tggaccagag cagaacgcag
     1921 gccagacgca cacaacagat aaagtgcagg ggatgcaaca cagaccagaa gcgagtaacg
     1981 ggctttttgc aggggatgca ggggatgttt cagccaggag ccccccaaag tgcgtaatgg
     2041 ttgccctgat gccgactgga accgcctagc gcactttttt gactgcgcga accgctctac
     2101 atcttcaata cagatcattt acgcattttc ctcgcgtagg acgggaaaca tcccctgcat
     2161 cccctgcatt tcgggtgtta cgccgtctgc gtcagcgttc catcccctgc actctgcctc
     2221 attcacgcga ctgatgcgaa tcacgcgcca cagcgcgacg tttaagcggt ctttcccggc
     2281 tgcttccaac cgatacccac cctctacccg gcccttctgc tgcttcaacc agtagccaat
     2341 atgaagcgcc cggcctgctt cctcggggtg tttttcccgt atggcgttca tcagttcaaa
     2401 cgaactgttt gcacgttcca gaacctcagc caccgtgacc gcacgcggcc cgagcgcttc
     2461 ataccaagcc ccgagaaacc gagcgagaac ccctaaccgg ttgtcgttct ccatcgcttc
     2521 ttcgataacc cgcgccgggt cgatcgtctc atagatgccc gcagcgccaa gccatgccac
     2581 gcactgacga accgagtttg accaatcctc gaaagacgcg gtattccccg gcacaattcg
     2641 cggggcacca atggcgaagt agccgagaag aacggttaaa gc
//
LOCUS       DAORHD010000659         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45187_length_2680_cov_2.276571, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000659 DAORHD010000000
VERSION     DAORHD010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45187_length_2680_cov_2.276571"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKO06_13660"
     CDS             <1..561
                     /locus_tag="PKO06_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70742.1"
                     /translation="HCHGRAAFSLIEVVLAMAILVFAFIPIMGALSSSMKGTHKDEKI
                     VQAINLAQTSLNAALQFPFNSLPPQAGTGAGTTASPWVFGGTIQPNFNYATSTLALRL
                     GQIGEFTVELRISDEPVRFVVPQYTAASRSTDVTANPATWNWTNYTTPWMNGIYHKYI
                     LTVSWTEPITGMKTYRLATFKARLVQ"
     gene            558..1094
                     /locus_tag="PKO06_13665"
     CDS             558..1094
                     /locus_tag="PKO06_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70743.1"
                     /translation="MMNAQAFHFRLTKRRANALLLSLVFAMILLQLAVSYSGLLRQSH
                     PQSQQIDERVKMDFLAQGLIEKAILKFQLFPADFYAAHDATRVASYGANATSFYLNNW
                     ITDANLSLINFAEASSTMSNIPMNCVVSSMSLLTDFKWNREALQARAETTYLSRSQGT
                     VSKEVVRTLQLTRQSNSP"
     gene            1112..2086
                     /locus_tag="PKO06_13670"
     CDS             1112..2086
                     /locus_tag="PKO06_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70744.1"
                     /translation="MSPRMFVIACVMSFCIFFSTICSSAVAAPNAAGASVGTVDLSLL
                     IIFHPQMSWYDPGQKAFRKQAPARMATDVARKGQELTDQARRIGEQKRLLEGRMEELR
                     RTFDRQTRALQDDFDKRISDLATGSAAVERNMFQVRNNQLHLKFNAEIKALSGQIQLL
                     DQQFNSTAGTDVTGQMTGPAETEQMFRGILQEIRNTVSQVAAAKGVGVVLDRGPSDLI
                     GTLNVSDSELPGENVYARLLSTAAGPHTGQDTAAAQGHFTYQASLGQTWLRSRMAILA
                     PFQQALFSQRVVVGGVDLTFDCLNTLLQQKRVTGPALQAILACVRGER"
     gene            2116..2634
                     /locus_tag="PKO06_13675"
     CDS             2116..2634
                     /locus_tag="PKO06_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70745.1"
                     /translation="MRHTREYQLAIWGLIVLFLASGAAAFALDARYSRNGECYLLLGD
                     SGTHQGVYRLNNPAADAIFVGRLYNPGTSIGISVDLNRNCYTFSEIVEPSYSTLSGNI
                     PRQVVDGTVSVNSCNWGYHAYEHYDHRGSGQTTRPIYRTPGASHSIGPGTPITAPLGN
                     PLPPGDTVFAGK"
BASE COUNT          572 a          788 c          721 g          599 t
ORIGIN      
        1 cattgtcatg gccgcgcggc attctcactc atcgaagtcg tgctggccat ggccattctc
       61 gtcttcgcct tcattccgat catgggtgcg ttgagcagtt cgatgaaagg gactcacaaa
      121 gatgagaaga ttgttcaggc gatcaacctg gctcaaacca gtctgaatgc cgcgcttcag
      181 ttcccgttca actccctgcc cccgcaggca ggcacgggag cagggacaac ggcttccccg
      241 tgggtgttcg ggggcacgat tcaaccaaac ttcaattatg cgacttcaac gttagctttg
      301 cgccttggtc agatcggtga gttcaccgtc gaactccgta tctcggacga acccgtgcgc
      361 ttcgtggtgc cccagtatac cgcggcgagc cgcagcacag atgtcaccgc aaatccggcg
      421 acctggaact ggacgaacta caccactccc tggatgaacg ggatttatca caagtacatc
      481 ctgaccgtga gttggaccga acccataacc ggaatgaaga cctatcgtct cgccacgttc
      541 aaggcaaggt tggtgcaatg atgaacgcac aagcttttca ctttcgcctg acgaagcggc
      601 gcgctaatgc tctgctgctt tctctggtct tcgccatgat cctgctgcaa ctggctgtct
      661 cctactctgg cctgctgcgg caatcacatc cacagagcca gcagatcgat gagcgcgtca
      721 aaatggactt tctcgctcag gggttgatcg agaaggccat tctgaagttt caactgtttc
      781 ccgccgactt ctatgccgct catgacgcca cccgagtggc ttcatacgga gcaaacgcga
      841 cgagttttta tctgaacaac tggatcaccg acgccaatct ctcgcttatc aacttcgccg
      901 aagccagttc aacgatgagc aacatcccca tgaactgcgt cgtttcgagc atgtccctgt
      961 tgaccgactt caagtggaat cgtgaagcac tgcaggctcg cgctgaaacc acctaccttt
     1021 ctcggtcgca aggaaccgtc agcaaggaag ttgtacgcac gctgcagctt acccgccaat
     1081 caaatagtcc gtgaccggag gaccgtcgtc tatgtcgcct cgaatgttcg tcatcgcctg
     1141 tgtcatgagt ttttgcattt tcttcagtac tatctgttcg tcagcggttg ccgctcctaa
     1201 tgcggctggc gcttctgtcg gaacagtaga tctgagtctt ctcatcatct tccatccaca
     1261 gatgtcctgg tatgatccgg gccaaaaagc ttttcgcaag caggctccag cgcgcatggc
     1321 cacggatgtt gcccgcaagg gtcaggaact gacggaccag gctcgtcgca tcggcgaaca
     1381 gaaacgactt cttgaaggtc gtatggaaga gttgcgtcgc acgttcgatc gccagacgag
     1441 agctctacag gacgacttcg acaagcggat ctccgacctg gccaccggca gtgccgcggt
     1501 cgaacgcaac atgtttcagg ttcgcaacaa tcagctgcat ctgaaattca atgccgagat
     1561 caaggccttg tcaggtcaga ttcaacttct cgatcagcag ttcaattcaa cggcaggcac
     1621 ggatgtgacc gggcagatga ccggcccggc ggagaccgag cagatgtttc gcggcattct
     1681 gcaggaaatt cgtaacacgg tctcacaggt tgccgcagcc aaaggtgtcg gcgtggtcct
     1741 cgaccgcggt ccatcagatc tgattggcac cttgaatgtg tccgattctg aacttccggg
     1801 cgaaaatgtg tatgcccgcc tgctctcgac agccgctggc ccacacaccg gccaggacac
     1861 ggccgcggcg cagggtcact tcacgtatca ggccagcctc ggacaaacgt ggctgcgcag
     1921 ccgtatggcc attctggccc cctttcaaca ggcgttgttt tcacagcgcg tggtggtcgg
     1981 cggagtggac ctgacgtttg attgtctgaa tacgcttctg cagcagaagc gcgtgacggg
     2041 accggcactc caggcgattc tggcgtgcgt tcgcggcgaa cgataaaagg gaaatctggg
     2101 aaggaggttc gggtcatgag gcacacaaga gaataccaat tggcaatctg ggggttgatt
     2161 gtcctcttcc tggcctcggg tgctgcggcc tttgcactcg acgcacgtta cagtcgcaac
     2221 ggagagtgtt acctgttact gggggatagc ggcactcacc agggggtcta tcgtctcaac
     2281 aacccggccg cagatgccat cttcgtcggt cgcttgtaca atccaggaac aagcatcggc
     2341 atctctgtcg acctcaaccg caactgctac accttttccg agattgtcga accatcgtat
     2401 tcgaccctgt caggcaatat tccccgtcag gttgtcgacg gtaccgtttc cgtcaactcc
     2461 tgtaactggg ggtatcacgc gtatgagcac tatgatcatc gcgggtcggg tcagacgacg
     2521 cgcccgatct atcgtactcc cggtgccagt cactcgattg ggcccggaac gccgatcact
     2581 gccccgttgg ggaacccact tccgccgggc gacacggttt tcgcgggcaa ataatggtat
     2641 gacattccca acgatagctg gtattcgacc tggcataccg
//
LOCUS       DAORHD010000660         2677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45261_length_2677_cov_3.060259, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000660 DAORHD010000000
VERSION     DAORHD010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45261_length_2677_cov_3.060259"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2677)
                     /locus_tag="PKO06_13680"
     CDS             complement(<1..>2677)
                     /locus_tag="PKO06_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70746.1"
                     /translation="AEEYTFQFSIDRTSLGVLPGRAEHYKVNRYWKPGSETPYDASMQ
                     PGRWIYDFWGGSRASVPHVYLDIPARDMLCVDYARKHPAGRLNQSAGWLGFGLSFLPR
                     STFGDPKIERWLSAVNTQLDHRQGSLAGGSGTYELKGGDIIRPDGRVKSANFAVLIDL
                     GKHRPMALIMGYCYTWNGLHDDSLKRHSARDHLEDFLDHLKIEFVDSIPPDERDLAHR
                     SPQLDWDAWRKKQQLKQEELEALAAQQKQIQGQSQAASQSAQVSQAAIEDSVKSAQAV
                     IAHYEKLIQRVETELSGLKPGDPRRSHLENERAMYRNDIENEQYYIETLKSGSAHYQR
                     FSYQDKLATDMREKMRIELSEMALKSRLFLQLPKFIERLSDSDKNAHATFKSELSTFP
                     KGSEGLQKLKDLSTAVRGHLLKQGQARMDIEASRASNADWTLFGAEVVVKAAEATLMV
                     GSLAIPGAANVMMGYGIAQGAFVDGSVVKAFENGVRAYSDGVDVAWAGVQGFFETERV
                     QLADGRIIQRPRGFRGAAEAAAFTYLLNKGMEFVGGALQKKFAGLSGSSGPGTTRPAD
                     PNHPLPDVARPDPAKAKDPSTPAGDKVVYKTNEAKYAESRTAIENRFNKDVPRKADNS
                     IDTSHQDYARHEAARKQQLADLDRQYDVILKREKLHHELDQCTRNFDKQIADSPDIPR
                     LADGTPDISSDAYRARRKQWEDQMNAIRARDEFKDTRMPVVENALSASNMTVKDHFSG
                     GRPKNVNSDIDVTPATQKQLDDFLANLARQGHKPASDSPPDRYVFPGLDMVVWKTESP
                     TSLLKKLGGKGEFTAEDMALLRRLYGSDGAIQGKVEWAFHAGSDKFGTIGGVSFTTNL
                     GPKDPLGAVLDNTKKFIDALVDLPFAIPTLVTGIML"
BASE COUNT          498 a          767 c          774 g          638 t
ORIGIN      
        1 gagcataatg ccggtcacca gggtcggaat ggcaaagggc aggtccacca gggcatcgat
       61 gaacttcttc gtgttgtcga gcaccgctcc gagagggtct ttggggccga gattcgtggt
      121 gaacgagacg ccgccgatgg tgccgaactt gtcactgccg gcatgaaacg cccactccac
      181 tttgccctgg atcgcgccgt cagatccata caacctgcgc agaagagcca tatcttcggc
      241 ggtaaactcc cctttgccgc cgagtttttt cagtagagag gtcggacttt cggttttcca
      301 gaccaccatg tcgagaccgg gaaacacgta acggtcgggt ggcgagtcgg aagcaggctt
      361 gtggccttgc cgtgccagat tggcgagaaa atcatcgagt tgtttctgtg tggccggggt
      421 cacatcgata tcgctgttga cgttcttggg acgcccgccg gagaagtggt ctttgacggt
      481 catgttcgag gctgaaagcg cgttttcgac caccggcata cgggtatcct tgaactcatc
      541 acgcgcgcga atcgcattca tttggtcttc ccattgtttg cgacgtgcgc gataggcgtc
      601 tgagctgatg tcaggagtcc catctgcaag tctcggaata tccggtgaat cggcgatctg
      661 cttgtcgaag ttcctggtgc actggtcgag ttcgtgatga agtttctcgc gtttgagaat
      721 gacgtcgtat tgccggtcca ggtcggccag ttgttgcttt cgagcggctt catgtctcgc
      781 gtaatcctga tgtgaggtgt cgatgctgtt gtctgccttg cgcggaacgt ccttgttgaa
      841 gcggttctcg atggcggttc ggctctcggc atacttggct tcgttggtct tgtagacgac
      901 cttgtcgccc gcgggggttg acgggtcttt cgccttcgcc gggtctggcc gcgcgacgtc
      961 gggtaatggg tgattcgggt cggccggtcg ggttgttcca gggcctgagc tgccggaaag
     1021 cccggcaaac ttcttttgca gagcgccgcc gacgaactcc atgcccttgt tcaacagata
     1081 cgtaaacgcc gcggcttcag ccgcgccacg aaacccacgc ggacgctgta tgatgcgtcc
     1141 atccgcgagt tgcacccttt cggtctcgaa gaatccctga acgcccgccc aggcgacatc
     1201 gactccatcg ctgtaggcgc gcaccccgtt ttcgaaggct ttcaccacgc ttccgtcgac
     1261 gaaggcgccc tgcgcgattc cgtaacccat catgacgttg gcggcgcccg gaatcgccag
     1321 cgagccgacc atcagcgtgg cttctgctgc tttgacgacg acttcagcgc caaacagcgt
     1381 ccagtcagcg ttgctggctc gtgaggcttc gatgtccatt ctggcctgcc cttgcttcag
     1441 caggtgccca cgcacggcgg tcgagaggtc tttcagcttc tgcaagccct cagagccctt
     1501 cggaaaagtc gaaagctcgg acttgaacgt cgcgtgcgcg ttcttgtcgc tgtcgctcag
     1561 gcgttcgatg aacttcggca gttgcaggaa cagacgtgac ttcaaggcca tttccgacaa
     1621 ctcgatccgc atcttttcac gcatgtcggt cgcgagtttg tcctggtacg aaaaccgttg
     1681 ataatgagca gaaccgcttt tcagcgtttc gatatagtac tgctcgtttt cgatgtcgtt
     1741 gcgatacatt gcccgctcat tttccagatg tgaccgccgc gggtcgccag gcttcagacc
     1801 gctcagctcg gtctcgacac gctgaatcag cttctcgtaa tgcgcgatga cggcctgagc
     1861 actcttgacc gaatcttcga tcgccgcctg cgacacctgt gcggattggc tggccgcctg
     1921 actttgaccc tgaatctgct tttgctgggc ggcaagagcc tcaagctctt cctgtttcaa
     1981 ctgttgtttc ttccgccagg cgtcccagtc gagctgggga gaacggtgtg ccagatcgcg
     2041 ctcatctggc ggtatggaat caacgaactc gatcttcaaa tgatcgagaa agtcctccag
     2101 atgatcgcgt gccgagtgcc tcttcaaaga atcatcgtgc aagccattcc acgtgtagca
     2161 gtaacccatg atcaacgcca tgggacgatg tttgccaagg tcgatcaaaa cggcgaagtt
     2221 tgcgcttttc acccgaccat ccggccggat gatatccccg cccttgagtt catatgtgcc
     2281 actgcctcct gccagagagc cttgacgatg atccagctgg gtgttgacag cactcaacca
     2341 gcgctcgatc ttcgggtcac cgaacgtgga gcggggtaga aatgaaagcc cgaacccaag
     2401 ccagcctgca ctctggttca gccgtccggc tggatgcttg cgtgcgtagt cgacgcagag
     2461 catatccctg gccggaatgt cgagatagac gtgaggaacg ctggcgcggc tgcctcccca
     2521 gaaatcgtag atccatcgtc cgggttgcat cgatgcgtca taaggggttt cgctgcctgg
     2581 cttccagtag cggttaacct tgtagtgttc cgctcgtcca ggaagaacgc cgagtgatgt
     2641 gcgatcgatg ctgaactgaa aagtatattc ttcggct
//
LOCUS       DAORHD010000661         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45354_length_2673_cov_4.091291, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000661 DAORHD010000000
VERSION     DAORHD010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45354_length_2673_cov_4.091291"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PKO06_13685"
     CDS             complement(<1..1090)
                     /locus_tag="PKO06_13685"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNV70747.1"
                     /translation="MEDRLRLIGLFEELAMLCDLAGENPFKARAFEKAARTLQSLDLP
                     LGQLLEEPPPGFGKGLLDKVREFLRTEHLQELDNLRGRFPATLFDLLKVQGLGPKKVK
                     LLYESLQIADLDALEKACQTHRIAELKGFGTKTEEKILAGIESVKRFRGQFRRFDVEK
                     LAADLIAQLTRQFPGMPCEAAGSFRRRKEIVKDLDLLVATDAPVPVMRAFVSLPGIRQ
                     VLQEGETKSAVILNNGLQVDLRVVPPESYGPALCHFTGSKEHNIRMRQIAQARGLKLN
                     EYGVFDGDRAEAMATEADVFAALSLPFIEPELREDLGEIEWAQTGRLPRLITREDLLG
                     TLHAHTTASDGTASLAGMSEAAGHRGLRW"
     gene            1238..>2673
                     /locus_tag="PKO06_13690"
     CDS             1238..>2673
                     /locus_tag="PKO06_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70748.1"
                     /translation="MLDSIKNALGIGKVPEAPTNPRPKPVAKTKTPVPPPKEFDVADL
                     FSGPAGAVQPAEAGATGLDEKLRQAYFWIINHAVISPFYDIAYEETPPSQYKLGDSHC
                     PIVLPTGQSYSSFVLLPLLTLALRRKCLLIGGPGRGKTQTAILMGILSGYSLKEVRRG
                     IQHGQPQMTIADLLGNPLPADLVGAKSMDEITIAWRKWLGMRVKIIDEYNRIPTRTQS
                     ALLTLMADNYAEILDQVFECPESAWYLTANDDAGGGTYQVIEALRDRIDVVVRALHFN
                     TRFLGDLLVRIEQKVKPEDIVPPQIIFTETELDAMQKQILKVRLPIPLRRRLEFFAAQ
                     FEFLEGGASMLEYRTKDNAACSGGDVKMLLNQETGKDRVREIGMQSVNGLSVRALMTA
                     MAFMKGMAFFHGEEEVSLEDMRQMLPFVLHDKLRPNLDARFFEEPGNAALRFDRIGWI
                     RRLFDQACAEYDRLNLDQDDPVAVLQHV"
BASE COUNT          545 a          779 c          799 g          550 t
ORIGIN      
        1 gccagcgcag accccgatgt ccggcggctt ccgacatgcc ggccaagctc gccgttccgt
       61 ccgaggccgt cgtgtgcgca tgcagcgttc caagcagatc ttcgcgtgta atcaaacggg
      121 gaagacgccc cgtctgagcc cactcgattt ctcccaggtc ttcgcgcagt tctggttcga
      181 tgaacggcag gctgagggcc gcgaagacgt ctgcttcggt ggccatggct tcggcgcgat
      241 caccgtcgaa gacgccatac tcgttgagtt tgagaccacg cgcctgggcg atctgccgca
      301 tgcggatgtt gtgctctttc gacccggtga aatggcagag cgcgggtccg tagctctcgg
      361 gggggacgac gcgcagatcg acctgcaaac cattgttgag aatgacggca gacttggttt
      421 cgccttcctg cagaacctga cgtataccag gcagtgagac gaaagcgcgc atgaccggca
      481 ccggcgcgtc ggtggcaacc agcagatcca gatccttcac gatctccttg cggcgacgaa
      541 aactgccggc ggcttcgcag ggcattccgg gaaactgacg cgtgagttgc gcgatcagat
      601 cggctgccag tttctcaacg tcgaaacgac gaaactgacc gcgaaaccgt ttcaccgact
      661 cgattccggc gagaatcttt tcttccgttt tggtgccgaa ccccttcagc tccgcgattc
      721 gatgggtctg acacgctttt tccagggcgt cgagatcggc gatctgcagc gattcataca
      781 ggagtttgac cttcttggga cccagcccct gcactttgag cagatcgaaa agagtggcgg
      841 gaaatcgtcc gcgcaggtta tcgagttcct ggagatgttc ggtgcgcaga aattcgcgca
      901 ccttgtcgag cagacccttg ccgaatccgg gaggaggctc ttccagaagc tgtccaaggg
      961 ggaggtcgag actctgcagc gtgcgtgccg ctttctcaaa agccctggcc ttgaagggat
     1021 tttctccggc caggtcacag agcatggcga gttcttcgaa caggccgatc agtctcagcc
     1081 gatcttccat tcgggcggct ccttccatcg atcgctcgtt gcctgatcca tgctaccagc
     1141 atttcccgtg aggatgcaaa gcgacgagga aacgcggtag aatgcccgtg ttgcttgcaa
     1201 tgatgttggg gaaccctaca gagaagtgag gctgcgcatg ctcgactcga tcaagaacgc
     1261 gctcggcatc ggaaaagtcc cggaggcgcc aacgaatccc cgtccgaaac cggtggctaa
     1321 gacgaaaacc cccgttcctc cgccgaagga gtttgacgtt gccgatctgt tttccgggcc
     1381 ggccggcgcg gtgcaaccgg cagaagccgg cgcgacggga ctcgatgaaa agctgcggca
     1441 ggcctacttc tggatcatta accacgcagt catcagtccg ttctatgaca tcgcgtatga
     1501 agagactccg ccgtcgcagt acaagctggg cgattcacat tgtccgattg tgttgccgac
     1561 cgggcagagc tactcgagct tcgtgctgct gccgctgctg acgctggctc tgcgacgcaa
     1621 gtgcctgctg attggtggcc ccggtcgggg gaagacgcaa actgcgatac tgatgggcat
     1681 tctcagcgga tattcgctga aagaagtccg gcgcggcatt cagcacggac agccgcaaat
     1741 gaccatcgcc gacctgctgg ggaaccctct gccggcggat ctggttggtg cgaagagcat
     1801 ggatgagatc accattgctt ggcgaaaatg gctgggcatg cgggtgaaaa tcatcgacga
     1861 gtataaccgt attccgacgc ggacccagag tgcgctgctg actctgatgg ccgacaacta
     1921 tgctgaaata ctcgatcagg tgttcgaatg ccctgaatcg gcctggtact taaccgccaa
     1981 tgacgacgcg ggcggcggca cctatcaggt tatcgaagcc ctgcgcgatc gcatcgatgt
     2041 ggttgtccga gccttgcatt tcaacacacg tttccttggt gacctgctgg tacgcatcga
     2101 gcagaaggtg aagcccgaag acatcgtgcc gccccagatc atcttcaccg agaccgaact
     2161 cgatgccatg cagaaacaga ttctcaaagt gcgtcttccc atacctctgc gtcgtcgact
     2221 tgagttcttc gcggcccagt ttgagtttct cgaaggcggc gcgtcgatgc ttgaataccg
     2281 caccaaagac aacgccgcct gctcgggcgg tgacgtaaag atgctgctca accaggaaac
     2341 cggcaaggac cgggtgcgcg agatcggcat gcagagcgtg aatgggcttt ccgttcgcgc
     2401 cttgatgacc gcgatggcct tcatgaaagg catggccttc tttcatggcg aagaagaggt
     2461 cagtctcgaa gacatgcgcc agatgctgcc gttcgtcctg catgacaagc tgcgccccaa
     2521 tctcgatgcc cgctttttcg aagagccggg caacgcggcg ctgcgctttg accgcattgg
     2581 ctggatccgt cggctgttcg atcaggcctg tgccgagtat gatcggttga acctcgatca
     2641 ggatgacccg gtcgccgtgc tgcagcacgt aca
//
LOCUS       DAORHD010000662         2670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45432_length_2670_cov_3.932696, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000662 DAORHD010000000
VERSION     DAORHD010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45432_length_2670_cov_3.932696"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PKO06_13695"
     CDS             <1..285
                     /locus_tag="PKO06_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70749.1"
                     /translation="EALSLLGVFALSFLVFHLGSALVRTDEALYRRESAVVPDPRSFP
                     AGPATVNASASPAPLPVRSPQNPGGTSIRKRIGGWVTLPATWTVKPREKQ"
     gene            415..>2670
                     /locus_tag="PKO06_13700"
     CDS             415..>2670
                     /locus_tag="PKO06_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70750.1"
                     /translation="MRTFYRSRHGQTLYLILLVAVLFVSGLYLASNYLSRSQARFSSQ
                     ATDELIAINLADSGINLAEASIRDQMNCFGGDWDSFIKTFGNSFLAGIYRPIILSLFV
                     DLPTRNLFLQECEDLEVELNRMLPPWRVDVKYRLSSVMPLGGTIAIGTIIKRWMPYIN
                     VEKQATIEIIATAYNPETGMSRLVAVEKELKVVDLTPPCSHYSFVFDSATPLPGAKPF
                     HFDAGTSGQNRAVEKADLAARNDSDDPDALLNGAFEPFPLSTKGYLSVESWDIGQVMN
                     IENFKPGEAFLKALFLPKGRVMLRGANAPKICKNANIVEWLSNVKPQQYYFTLDQFTF
                     EFPTRPLTIDLQRLFQEYYGQEVGTAVFASLSEGALWGIKVPPRIDFPRFGFNERMLN
                     DFFGIVQLFIGGNWPAKRTLGGDPSINDCLMAFSKEVLKSATGIDLRLSASPDTVDMA
                     RQIENLDLLGKTEQVISMLQSLYGEKGGIDNYDIWEFLMAPYGKGSLTTNQTGAVSKP
                     DKMGSTTIANGWSLPILFRNNGHFWGCLIDPSSVVSGILNFSPKDLANDITTDWTKGA
                     KRFEEFKSTSIKSMQAGKDLGQAAKNQNFRQAAQSLKTLTQLARTTGGDISTDLNNLA
                     ENLVLNPVMDGGVSVVLNTFLDLVPRQWLEAAQQKIVGDITGIKIINCLLFPRMEGKI
                     YSGWYQMAACPTGYWTDLNAVGVGVAGTGGAGGGATGGGTAGTTGGTVVPGVGNAIGA
                     AGGTAGGGAGGG"
BASE COUNT          560 a          818 c          748 g          544 t
ORIGIN      
        1 gaggctcttt ctctgctggg agtgttcgct ctgagttttc tggtctttca cctggggtca
       61 gcgctggtgc gaaccgacga ggccttgtat cgtcgcgagt cagctgtcgt tcccgacccg
      121 cgaagtttcc ccgcgggtcc cgccaccgtg aacgcctcag cgtctcctgc accgctgccg
      181 gtgcgttctc cgcagaatcc cggcggcaca tcgatccgca aacggatcgg aggctgggta
      241 actctccccg ccacctggac cgtcaaaccc cgagagaagc aataggtccg gatgtgtctt
      301 gagagcactg cagcgaatgc tccctgtact ttcccgacag tgagcgacag ggagatgctc
      361 gggattcgta gtcgctgatt ggcaagtgtt caacccgtac gttacactgc cttcatgcgc
      421 acgttttatc gatctcgaca cgggcagaca ctgtatctga ttcttctggt cgccgttctg
      481 ttcgtttcgg gtttgtacct ggcatcgaac tatctgagcc gcagtcaggc acgtttttca
      541 tcgcaggcaa ccgacgaact gattgccatc aatctggcgg acagcggaat caatctcgcc
      601 gaagcctcga ttcgcgacca gatgaactgt ttcggcggtg actgggactc cttcatcaag
      661 acattcggca attcatttct tgccggtatc tatcggccaa tcatactgtc gctgttcgtc
      721 gacctcccga cccggaacct gttccttcag gaatgcgagg atctcgaggt cgaactgaac
      781 cgaatgctgc caccctggcg cgtcgacgtc aagtatcgcc tcagcagcgt catgccgctt
      841 ggcggcacca ttgcgatcgg tacgatcatc aaacgctgga tgccttacat caacgtcgag
      901 aagcaggcaa ccatcgagat tatcgccacg gcttacaacc ccgaaaccgg aatgagtcgc
      961 ctggtcgcgg tcgagaaaga actcaaggtc gttgacctga ccccgccctg ctcgcactat
     1021 tccttcgtct tcgactcggc gacgcctctg cccggcgcca agcctttcca ctttgatgcc
     1081 ggaacctctg gccagaatcg tgcggtcgaa aaagccgatc tggccgcacg caacgattct
     1141 gacgacccgg acgctttact gaacggagct ttcgaaccgt ttccgctctc gaccaaaggc
     1201 tatctctcgg tcgaaagctg ggacatcggg caggtgatga acatcgagaa cttcaaaccg
     1261 ggtgaagcgt ttctcaaggc tctttttctg cccaaggggc gcgtcatgct gcgcggggcc
     1321 aacgccccga aaatctgcaa gaacgccaac atcgtcgagt ggttgagcaa cgtcaagccg
     1381 cagcagtact actttaccct cgaccagttt accttcgagt ttcccacccg accgctgacc
     1441 atcgacctgc agagactctt tcaggaatac tacgggcagg aagtcggaac cgccgtattc
     1501 gcctctctca gcgaaggagc tctctggggt atcaaggttc cgccgcgcat cgattttccc
     1561 cgcttcggat tcaacgagcg catgctcaac gacttcttcg ggatcgtaca actctttatc
     1621 ggaggaaact ggccagcaaa gcgcactctt ggcggagatc cctctatcaa cgactgcctc
     1681 atggcgtttt cgaaagaagt gttgaaatcg gcgaccggca tcgacctgcg attgtctgcc
     1741 agtcctgata cggtcgacat ggcgcggcag atcgaaaacc tggatcttct cggcaagacg
     1801 gaacaggtga tcagcatgct gcagagcctg tacggtgaga aaggcggcat cgacaactac
     1861 gatatctggg agttcctgat ggccccctac ggcaaagggt cgctgaccac caaccagacc
     1921 ggtgccgtca gcaagccgga caagatgggc tcgaccacca ttgccaacgg ctggtcgctg
     1981 ccgattctgt ttcgcaacaa cggccacttc tggggctgcc tgatcgatcc atccagtgtg
     2041 gtttccggca tcctgaactt cagtccgaaa gatctggcca acgacatcac cacagactgg
     2101 accaaaggcg ccaagcggtt tgaggagttc aagagtacca gtatcaaatc gatgcaggcc
     2161 ggcaaggatc ttggtcaagc cgcaaagaac cagaactttc ggcaggcggc acagtctctc
     2221 aagaccctga cccagctcgc gcgaacgacg gggggcgaca tctcgaccga cctgaacaac
     2281 ctggccgaga atctggttct caatcccgtc atggacggcg gcgtctccgt cgtcctgaac
     2341 acgttcctcg atcttgtgcc caggcagtgg ctcgaggctg cgcaacagaa gatcgtcggc
     2401 gatatcaccg gaatcaagat catcaattgt ctgctgtttc cgcgcatgga aggcaagatc
     2461 tattccggct ggtatcagat ggccgcctgc cccaccggct actggaccga tctcaacgcg
     2521 gtcggcgtcg gagtagccgg caccggcgga gccggcggcg gggcaaccgg gggcggcacc
     2581 gcaggcacca ccggcggaac ggtggtcccg ggagtcggca atgcgatcgg cgcggccggc
     2641 ggcacagcag gcggaggagc cgggggcggc
//
LOCUS       DAORHD010000663         2668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45474_length_2668_cov_5.071948, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000663 DAORHD010000000
VERSION     DAORHD010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45474_length_2668_cov_5.071948"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2115)
                     /locus_tag="PKO06_13705"
     CDS             complement(<1..2115)
                     /locus_tag="PKO06_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70751.1"
                     /translation="MTRFWCSLLLVLVTMTVSAVETEVFSDASGTWFRVSLTAEELQS
                     ISPADITAVSASPDTNRVVTSPSRGTPEVAPLKRKACQANMKVLASAIEMYNMDHKDL
                     LREITPKTIAVLEQEKYLRAPFRSPSPNCRYSNTDKHGYYIRVTCSVHGDLEQTWEEH
                     RRREDEQRKSLDTKPGLVVLGQLTSKSPARLKLTNRRTGKSLRDLQIDTAQATPGTEE
                     IKTQWLETWAMDLMLMAGRTPGNPGFKFIMLQEAARMLGTSPAADSPDRRNRLQQADL
                     YSLTTGALAIQEALQLDRMTNPRAHRSDDRHEISKLVGPQIKSHPFAEMIGKRVPQVL
                     PIDALVPAHFYACHFSSIGRQMEFGDLLDQWGTSLLHTMQVAAHDAQIKEKFQDQLCL
                     KPSELTRLFGDQVIADLTICGADPFLHEGTDLTVIFNLKNATLFEANAQKNFAEARSR
                     VPGCREETIDLDGVQVHSLTSIDTKLYSLSCTLDNFKVYSNSRTALKTVISTFRGKTP
                     SLAASDDYRYMRLVFPPNTSKEDVFLYLSDAHIRQLVGPVWKITRQRRIQCLASLRLI
                     HDAATLFQLDGKPGIPTLERLVKDNLLPETLLWCPDHGTYTYGGKPEDDPVCSIHGRM
                     RHLTPIIEYSPKLVSVEEKQDYENFVREYNQYWRQFFDPIGIRGTLATDGILLETCIL
                     PLVENSIYDGFKLLSGGEPVEID"
     gene            2239..>2668
                     /locus_tag="PKO06_13710"
     CDS             2239..>2668
                     /locus_tag="PKO06_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNV70752.1"
                     /translation="MAWHRVCVWIFVGVLLACTSGVGYGGEAVLAPNDVCFGVNLYKQ
                     VGTKPGNLFFSPLSFTTALAMTQTGARGQTEKEMSTAGCLPYDAATILSLFGDLLKKL
                     NEAGGTNELRVANSLWAQHKYPFLPEYLKSVQEAFSARVEN"
BASE COUNT          544 a          676 c          844 g          604 t
ORIGIN      
        1 gtcgatttcg accggctctc cgcccgaaag cagtttgaag ccgtcgtaga tgctgttttc
       61 caccagaggc agtatgcagg tttcgagcag aatgccgtcg gtcgccaggg ttccgcgaat
      121 gccgataggg tcgaagaatt gtcgccagta ctggttgtac tcacggacaa agttctcgta
      181 atcctgcttt tcttcaaccg aaaccagctt cggcgaatac tcgatgatgg gggtcagatg
      241 acgcatgcgt ccgtgaatcg agcagacggg gtcatcctca ggttttcccc catacgtata
      301 ggtgccatga tccggacacc agagcaacgt ctcgggaagc aggttgtcct tgaccaggcg
      361 ctcgagagtc ggaatgccgg gttttccgtc gagttgaaac agcgtggcgg catcatgaat
      421 cagtcggagt gacgcgagac attgaattcg tcgctgtcgc gtgattttcc agactggacc
      481 taccagttgt cgaatgtgcg cgtcggacag atacagaaac acgtcctctt tcgacgtgtt
      541 cggtgggaag accaggcgca tataccggta gtcgtcgctg gcagccagtg agggggtttt
      601 cccacgaaac gttgagatga cggtcttcag agcggtgcgc gaatttgaat agactttgaa
      661 attgtccagg gtgcaggaga gtgaatagag cttggtgtca atcgatgtga ggctatgaac
      721 ctggacaccg tcgagatcga tcgtttcttc acgacacccg ggaacacgcg aacgagcttc
      781 ggcaaaattc ttttgggcat tcgcttcgaa cagggtggcg ttcttgagat tgaagatgac
      841 ggtcagatcg gttccttcgt gcaggaacgg atcggctccg cagatcgtca ggtcggcgat
      901 cacctggtcg ccgaagaggc gtgtcagttc ggacggtttc aggcagagct ggtcctgaaa
      961 tttttccttg atctgggcgt cgtgcgcggc gacctgcatc gtgtgcagga ggctggtgcc
     1021 ccattgatcg agaagatcac caaactccat ctggcgaccg atgctggaaa agtgacaggc
     1081 gtaaaagtgc gctggcacca gagcgtcgat cgggagcacc tgaggcacgc gttttccgat
     1141 catctccgcg aaaggatgtg atttgatctg tggcccgacc agtttggaaa tctcatgacg
     1201 atcgtcgctg cgatgagccc gcgggttggt catgcggtcc agttgcagcg cctcctgaat
     1261 ggccagggct ccggtggtca acgaatagag atcggcctgc tgcaagcggt tgcgccggtc
     1321 aggagagtca gctgcgggag aggtgcccag catgcgtgcg gcctcctgca gcatgatgaa
     1381 cttgaaacct ggattgccgg gagtgcgccc ggccattaac atcaggtcca tggcccacgt
     1441 ttcgagccat tgggttttga tctcctcggt tcccggagtg gcctgggccg tatcgatctg
     1501 cagatcgcga agactcttgc cggtgcggcg gttggtcagt ttcagccggg ctggggactt
     1561 ggacgtgagt tgtcccagaa cgaccagacc gggtttcgtg tcgagcgact tgcgctgctc
     1621 gtcctcgcga cgccggtgtt cttcccaggt ctgttcgagg tcgccgtgaa cggagcaggt
     1681 cacgcggatg taatacccgt gcttgtcggt gttcgagtat cgacagttgg gtgacggtga
     1741 acgaaaggga gcgcggagat acttctcctg ttccagaacg gcaatggtct tgggcgtgat
     1801 ctcgcgcaga aggtctttgt ggtccatatt gtacatttcg atggcgctcg ccagaacctt
     1861 catgttggct tggcatgctt tacgcttcaa cggcgcgact tccggcgtgc cgcgactggg
     1921 actcgtgacc acccggttgg tgtccgggga agcggaaacg gcagtgatgt ccgcaggaga
     1981 aatcgattga agttcttcgg cggtcagaga gacccgaaac caggttccgc tcgcatcgga
     2041 aaacacctcg gtttcaacgg cactgacggt catcgtgacg agcacgagca acagggaaca
     2101 ccagaatcgc gtcataggtc tcctcccgaa agcgtgtgtt ctcatcatcg tatcacaggt
     2161 gaggattgac gaggggggcg aatcgggcta ctctgctgcg agaaggaatt caactctgtt
     2221 tcaggaggaa tgatcatcat ggcgtggcat cgtgtgtgtg tgtggatatt tgtgggcgtg
     2281 cttcttgcct gcacgtccgg ggttggatat ggtggagaag cggttcttgc gccgaacgat
     2341 gtgtgctttg gggtgaactt gtacaaacag gttggcacca aacccggcaa cctgttcttt
     2401 tcaccgctga gtttcacgac tgcactggcc atgacccaga ccggcgctcg aggtcagacc
     2461 gagaaggaaa tgagcaccgc aggctgcctg ccgtatgacg ccgcgaccat tctgtcgttg
     2521 tttggcgacc tgctgaagaa gctgaacgag gcggggggta cgaacgaact gcgcgtggcg
     2581 aactcgctct gggcgcaaca caagtatccg tttttaccgg aatacctcaa gtcggtgcag
     2641 gaagcttttt cggcacgggt cgagaacg
//
LOCUS       DAORHD010000664         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45771_length_2657_cov_3.867025, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000664 DAORHD010000000
VERSION     DAORHD010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45771_length_2657_cov_3.867025"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2604)
                     /locus_tag="PKO06_13715"
     CDS             complement(<1..2604)
                     /locus_tag="PKO06_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70753.1"
                     /translation="MQNIATRFVNQDTAYLVRFISRLEKQIPDAVRWVAWDSNRNLIE
                     LPSQVSLPGSRRWEKIIQDFFANPDVPTHREPERRNQLEQQLQALVGPRLRATDILVS
                     RGEAVFGEFLGKTCLVYWVNWLLPDGKQPSPTTCVSGFFAVVFLDKLPNNFWSRMAFS
                     GYAPADFRDLSFPCALITTNPSRHVLQPPLPRTRRFVARLQAEFSRTRTVHALIDDWM
                     AVPLPIHSSKNEQIFVFKNLQPVLQSSRNAFRSLQTALLLLLTIGMTGVWVLKSGAGL
                     NLSLHWRIAGYFLLAVGLPMFGLVQATITIGRYQFDELRERLLKRMRESALLMERLSN
                     SYAPTHAQQMALRVSDFCHNSKSLSDMHTHLQQLVQSRAVSNYFLSDDSGNLAFVGQP
                     AVDRYYLTMMKTVLRDEMIKQIGSHEETGRPLGELMIDEVGKGAQVIESGEGNLLSRP
                     GRWEPFRLSRTNAFVMRCLVWLSGKPHVLVLVAHEVHLEQPLFRQEVMKQRFVSEDRL
                     DSIELAFSSRSPYEWPHLPPSSPLCLNSQVTELLNAEAEAEFDIDLASETFLLYAPAR
                     SNRIYRPIFFTSLSQVYALAEARRRQLFLTAGAALGVALLLGLLLASRLLGPIRRIDE
                     ALGLVTRGDLDIELPVESRDEIGHISETFNQMVSGLRERKRMQAYVSDAVLAAVKDTA
                     NTRWQAGEVIEATILFSDIRGFTTLCEAQTPEVMFSLLNDFLGGIEPVLSRFHGEVDK
                     FIGDAVMAVFRTPGPDQALQAVHAALAMKEYLSEFNAMRVKSGLFPINIGIGISTGRV
                     MQGDVGSQRRKDLTVIGDEVNLAARLETASKTGLHTQIVISESMWNLVRSQVETVEMP
                     STTVKGKQQTVR"
BASE COUNT          590 a          739 c          792 g          536 t
ORIGIN      
        1 gcggacggtc tgctgcttgc ctttgacggt cgtgcttggc atttccacgg tttcaacctg
       61 cgaacgcacg aggttccaca tactttcgga gatcacgatc tgagtgtgca aacccgtttt
      121 tgaagcggtt tccagccggg cggcgaggtt gacttcatcg ccgatgaccg tgagatcttt
      181 gcggcgttga ctcccgacat cgccctgcat gacgcgaccg gtgctgatgc cgatgccgat
      241 gttgatggga aacagccctg atttgacgcg catggcattg aattccgaca ggtactcttt
      301 catggccagc gccgcgtgaa ccgcctgtag agcctgatcg ggacccggcg tgcgaaagac
      361 cgccatgaca gcatcaccaa tgaacttgtc gacctcaccg tgaaatcgtg agagtaccgg
      421 ttcgatgccg cccagaaagt cattcagcaa cgaaaacatc acttcaggag tttgagcctc
      481 acacagggtg gtgaatccgc gaatgtccga aaagagaatg gtggcttcga tgacttctcc
      541 cgcttgccag cgagtgttcg cggtatcctt caccgcggcc agaacggcat cggaaacata
      601 ggcctgcatg cgtttacgct cacgcaaccc cgacaccatc tgattgaatg tctcgctgat
      661 gtgcccgatt tcgtcgcgac tctccaccgg aagttcaatg tcgagatcgc cgcgcgtgac
      721 caaccccagt gcctcgtcaa tacggcgaat cgggcccagc aatcgactcg ccagcaacag
      781 ccccagtagc agtgcgactc cgagggctgc gcctgctgtc agaaacaact gccgacggcg
      841 tgcttcggcg agcgcataga cctgagacag cgaggtgaaa aagatcggac ggtagatgcg
      901 gttcgatcgt gccggcgcgt agagcagaaa ggtctcgctg gcgagatcga tgtcgaactc
      961 cgcttccgct tccgcgttga gaagttccgt tacctgggag ttgaggcaga gaggggagct
     1021 gggcggcaga tgcggccatt catacggcga acgcgatgag aaggccagtt cgatcgaatc
     1081 aaggcgatct tcggagacaa agcgttgttt catgacctcc tgcctgaaca gcggctgctc
     1141 gagatgcact tcatgggcga cgagaaccag gacatgcggt ttgccggaga gccagacgag
     1201 gcatctcatg acgaaggcat tcgtccgaga cagtcgaaat ggctcccagc gccctgggcg
     1261 agagagaaga ttgccctcac ccgactcgat cacctgagca cccttgccga cctcgtcaat
     1321 catcagctca ccgaggggac gaccggtttc ttcatgactg ccgatctgct tgatcatctc
     1381 gtcacgcaac acggttttca tcatcgtcag gtagtaccgg tcgacggcag gttgtccgac
     1441 gaaggcgagg ttcccggaat cgtcagagag aaaatagttg ctgacggctc tgctttgtac
     1501 tagttgttgc agatgcgtat gcatatctga aaggcttttt gaattgtgac aaaagtcgct
     1561 gacgcgaagc gccatctgct gagcatgagt cggtgcgtac gagttggaaa gacgttccat
     1621 caggagggcc gactcgcgca tgcgcttcag cagacgttct cgcaactcgt cgaattggta
     1681 cctgcctatg gtaatggtgg cttgaaccag gccgaacatg ggcaggccga cggccaggag
     1741 aaagtagccg gcgatacgcc agtgcagcga gaggttgagg cccgcacccg atttgagcac
     1801 ccagactcct gtcatcccga tggtgagaag aaggaggaga gccgtttgca gactgcgaaa
     1861 ggcgtttcgc gacgattgca gtaccggctg caggttcttg aacacgaaaa tctgttcgtt
     1921 cttactgctg tgaatcggca ggggaacggc catccagtcg tcgatcaggg cgtgaaccgt
     1981 tctcgttcga gaaaactccg cctgcagccg cgccacgaaa cggcgcgttc gcggcagcgg
     2041 cggctgcagc acgtgccgac tgggattggt ggtgatcagc gcgcacggaa aagacagatc
     2101 acgaaagtcg gctggggcgt aaccggaaaa cgccatccgg ctccagaaat tgttcggcag
     2161 tttgtcgagg aatacgacgg cgaagaaacc ggaaacacac gtggtcggtg acggttgttt
     2221 cccatccggg agcagccagt tgacccagta gaccaggcac gtcttaccca gaaactcgcc
     2281 aaacacggct tcgccgcgtg acaccaggat gtcggtggct cgcagtctcg gtccgacgag
     2341 cgcctgcagc tgctgctcga gttgattgcg ccgttcgggt tcacgatgtg tcggcacgtc
     2401 cgggttggca aagaaatcct gaatgatctt ctcccagcgt cgagatcctg gaaggctcac
     2461 ctgagacggc agttcgatca ggttccggtt cgagtcccag gcgacccacc ggacggcatc
     2521 gggaatctgt ttctcaagac gggaaatgaa gcgtacgaga taagctgtat cttgattgac
     2581 gaaccgcgtg gcaatgttct gcatgacact ctctggctgc agggcatgac gaaccttgat
     2641 ccatcggcct tccaaca
//
LOCUS       DAORHD010000665         2653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45876_length_2653_cov_2.686297, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000665 DAORHD010000000
VERSION     DAORHD010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45876_length_2653_cov_2.686297"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="PKO06_13720"
     CDS             <1..1184
                     /locus_tag="PKO06_13720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV70754.1"
                     /translation="TIFPAFRQVWLPILARQAAASLESAILYERMFKRLLVLSNVFIL
                     GKEIVTNIDLNSLVDKFLSIARDGSNSDIAAIYLLREPEAEPHFTRVQIGGASGKPLS
                     GPLVVSPLIRQVCADQKPITVDSLSQSPYADTEPGKFPGVLMQNTFAMPLVVHDRFLG
                     VIQMANKIGRGGFTSEDLDLLKILSGQIAFVIQNANLFQNLQSAYINTLGALTSAIDA
                     KDSYTHGHSERVTELSVQLGREVALNTEDLENLRIAGLLHDIGKIGIPEGILNKPGRL
                     TDEEFDIIKSHPELGVRILKKVDFLHPIIPFIQHHHERFDGRGYPTGLSGEKIPLCAR
                     IITVADTYDAMTSDRPYRKALDQQTALEEVLRCKGTQFDPQIAEAFVKMMNRQTSGMI
                     P"
     gene            1187..>2653
                     /locus_tag="PKO06_13725"
     CDS             1187..>2653
                     /locus_tag="PKO06_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV70755.1"
                     /translation="MFLTGHARFFERLFVGIIILTLSCFAYYFTLSDLDELRERFENR
                     RTVLVKIVAREIKEALGRKGDLSARLSNLISEEAIAYALVQQSEEELLAKAENTSVAI
                     GVMKDLEKNALQASSLRYTPFVDLSGSLSLIEAAIPVFLDSGRRVVLRIGFYKGDEDE
                     RTRQIWFRNTLLFSTLLLGVLTYLLLRRRGSFGLQITWMGGAGLLILLVFLSCRFTIH
                     QWYDRYWRQSFIRNSMNQAKILAISARRFFLSGEDKDLRDLQSLLEFDDTFAYVGVIR
                     DDQFLYHTDPAMKGKPITDPAYRQSIDQNKPLCKKADDDMYEVFVPVLDGLHQLGTLK
                     IGLRYSAGFEPLKILRNHILLIFLSGFSIALFLIYLLTTRVTRDVTSFIRAMEQVTAG
                     DLRQNIYLDRNDEFGQMAQAFNFMLMSLKERDLLNKGMQHYVSKSIVDKTMKALTAQE
                     KSGEKIFTVNLFVSFFGINEAIGGKAAPEVFQAVRDVTQ"
BASE COUNT          554 a          801 c          668 g          630 t
ORIGIN      
        1 gtacgatatt cccggcgttc agacaagtgt ggctgccgat tctggcgcgt caggctgctg
       61 cctcgctcga aagcgcgatt ctctacgaac gcatgttcaa gcgtctgctg gttctgagca
      121 atgtgttcat tctcggcaaa gaaatcgtca ccaacatcga tctcaacagt ctggtcgaca
      181 aatttctctc gatcgcgcgt gacggttcaa actccgacat tgccgcgatc tatctgctgc
      241 gtgagcctga agccgagccg catttcacgc gggtacagat tggtggtgcc tccggcaagc
      301 cgctctccgg tccgctggtc gtctctccgc tcatccgcca ggtttgtgcc gatcagaagc
      361 cgatcacggt cgattctctt tctcaaagcc cctatgccga caccgagccc ggcaagttcc
      421 cgggagtgct gatgcagaat acgttcgcca tgcctctggt cgtccatgac cgctttctcg
      481 gcgtcatcca gatggcgaac aaaattggtc gcggtgggtt cacctctgaa gatctcgatc
      541 ttctcaagat tctcagcggt cagatcgcgt tcgtcattca gaacgccaac ctgtttcaga
      601 atctccagag cgcttacatc aacacactcg gtgcgctcac ttccgccatt gatgccaaag
      661 atagctacac ccacggccat tccgagcgcg tcaccgagct ctccgttcaa ctcggccggg
      721 aggtcgccct gaacaccgaa gatctcgaaa atctgcgtat cgccggtctg ttgcacgaca
      781 ttggcaagat cggcattccc gaaggcattc tcaacaaacc cggccgtctc accgacgagg
      841 aattcgatat catcaagtcc cacccggagc ttggggtgcg cattctgaag aaagtcgatt
      901 ttctgcaccc gatcatcccc ttcattcagc atcatcacga acgtttcgat ggccgaggtt
      961 atccgaccgg gctttcggga gagaagattc cgctttgcgc gcgcattatc accgtcgccg
     1021 acacgtacga tgccatgact tctgatcgcc catatcgcaa agctcttgat cagcagaccg
     1081 ctctggaaga agtcttgcgc tgcaagggca cgcagttcga tccgcaaatc gccgaggcgt
     1141 tcgtcaaaat gatgaatcgc cagacgtccg gtatgattcc ctgaccatgt ttctcaccgg
     1201 ccatgcccga tttttcgaac gccttttcgt cggcatcatc attctgacgc tctcgtgctt
     1261 cgcctactac ttcacgcttt ccgatcttga cgagctgcgt gaacgtttcg aaaaccggcg
     1321 caccgtgctc gtcaaaatcg tcgcccgtga aatcaaggaa gctctgggtc gcaagggtga
     1381 tctttcagct cgcctgtcga accttatttc ggaagaagct attgcctatg ctttggtgca
     1441 gcaatccgag gaggagctgc tggcgaaagc cgagaacacc agcgtggcta tcggcgtcat
     1501 gaaagacctc gagaaaaacg ctctacaggc ttcctcactg cgctacactc cgtttgtcga
     1561 tctttcgggc agtctttcgc tgatcgaagc ggcaattccg gtgtttcttg attccggtcg
     1621 ccgggtggtt ctccgcatcg gcttctacaa gggtgacgaa gacgaacgca ctcggcagat
     1681 ctggttccgc aacaccctgt tgttctcgac cctgcttctc ggcgtgctga cgtatctgct
     1741 gctgcgtcgt cgcggcagtt tcgggctgca gatcacctgg atgggtggcg ccggactgct
     1801 tatcctgctc gtgttcctct cgtgtcgctt cacgattcat cagtggtatg atcgctattg
     1861 gcgccagagc ttcattcgca acagcatgaa ccaggcgaag attctggcga tttctgcgcg
     1921 ccgcttcttt ctcagcggcg aagacaaaga tctgcgcgac cttcaatccc tgctcgagtt
     1981 cgacgacacg ttcgcctacg tcggtgtcat tcgcgatgac cagttcctct atcacactga
     2041 tccggccatg aagggcaaac ctattaccga tcccgcctac cggcagagta ttgatcagaa
     2101 caagcccctc tgcaaaaaag cggacgacga catgtatgag gtatttgtac cggttctcga
     2161 tgggcttcac cagctcggaa cgctgaaaat cgggcttcga tattcggccg gctttgaacc
     2221 gctgaagatc cttcgcaatc atattctgct cattttcctc agcggcttct cgatcgctct
     2281 gtttctcatc tatctgctga ccacgcgggt cacccgcgac gtcacgtcat tcattcgtgc
     2341 catggagcag gtcaccgccg gcgatctgcg ccagaacatc tatctcgacc ggaacgacga
     2401 gttcggccag atggcccagg ctttcaactt catgctgatg agcctcaaag agcgagacct
     2461 gttgaacaaa ggtatgcagc actatgtcag caagagcatc gtcgataaga cgatgaaagc
     2521 gctcaccgcc caggaaaagt cgggcgaaaa gatcttcacc gtcaatctct tcgtctcctt
     2581 cttcggcatc aacgaagcca tcggcggcaa agccgcccca gaggtttttc aggccgtacg
     2641 cgatgtgacc cag
//
LOCUS       DAORHD010000666         2651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45927_length_2651_cov_2.571263, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000666 DAORHD010000000
VERSION     DAORHD010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45927_length_2651_cov_2.571263"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PKO06_13730"
     CDS             <1..446
                     /locus_tag="PKO06_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNV70756.1"
                     /translation="VIVLASAAAVYGPDLALYALIKTYISSEALDKFMEGFSDSRQVM
                     IISKQAETLSWGIMEELHRGVTLLAARGAYTNRPSEVILTAIRRRELAVLEELVYRID
                     PGAFMIITDARRILGKGFDDLEKIVAQAKVAVGETLPETDVPAKA"
     gene            complement(447..1061)
                     /locus_tag="PKO06_13735"
     CDS             complement(447..1061)
                     /locus_tag="PKO06_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HNV70757.1"
                     /translation="MPNPGWFKNRLQDPFFRRAHRENYRSRAVYKLQEIQEKYQLIRS
                     GNRILDLGAAPGSWTQYAVAQAGRTGKVIGIDLLPIQPLEGATLLEGDINDPVMQEQI
                     QAFAPKGFDLIMSDMAPNTTGVHHADAGNSVELVHVALDLSKIWLKNGGHFVAKVFEG
                     TDFRELHERFKKLFGFAKSFKPKASLAASRETYLVGKEFRASKL"
     gene            complement(1074..>2651)
                     /locus_tag="PKO06_13740"
     CDS             complement(1074..>2651)
                     /locus_tag="PKO06_13740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV70758.1"
                     /translation="ADRVTLTLLHTSDTHGHIASDIDAADTTGSGHRVSGFPLLKAQV
                     QKIKLDALRQGNRALLLDAGDYFQGTAIVEETQGQVMIDLMNRMNYLAAGIGNHDLDY
                     GLARLKDVLRSARHPILCCNVFETATGRLMASARSHIRVPYKGHQIAIIGALDPRTPD
                     ISMAENVAGLEFRDPVPILNELISRLRTQGVDVFVLLSHLGIEQDRQIAETVEGIDLI
                     LGGHSHTEMRELEYAGKNRVPIAHPGYDTRYVSRVDLHLLKGERPAVTFTPHFLSTAH
                     LEEDAGMQTVIGEKLSSIAAKVKRHLGTCRATLVRGVIGGDSPEGNFVADAIREGTGA
                     EIGLTNVGGVRFPIASGPVIWDQIFQLQPFNNLVEVVPMTGAQIVKILEEGLSVPFQQ
                     ADAADKEYARKHFRLDISGLRRDFMAAFGYLVPSNLLVTFDPERPPMQRIVKLTLADD
                     SPLDLKRVYKVAFNSYLSQGGDGYKYLKNWPGKINSRKLVRDLIVEKIERDQVIGPPA
                     EQRMINLKLSCVPITGE"
BASE COUNT          523 a          767 c          783 g          578 t
ORIGIN      
        1 atgtcatcgt gctcgcgtca gcggcggccg tctacggacc cgatctcgct ctctatgccc
       61 tgatcaagac ctacatctcc agcgaagcac tcgacaagtt catggaaggc ttctccgact
      121 cccgacaggt gatgatcatt tcgaaacagg ccgagacgct gtcctggggg atcatggaag
      181 aactgcaccg tggcgtgacc ctgctggccg ctcgtggggc ttacaccaat cgtcccagtg
      241 aggtcattct gaccgccatt cgccggcgcg aactggccgt tctcgaggaa ctggtgtatc
      301 gcattgatcc tggggctttc atgatcatca ccgatgcgcg acgcattctc ggtaaagggt
      361 ttgatgatct cgaaaaaatc gtggcgcagg ccaaggtggc ggtcggtgag acgctgccgg
      421 agaccgatgt tcccgcgaaa gcctgatcag agcttgctgg ctcggaattc cttgccgacc
      481 agataagtct cgcgactggc ggccagtgag gctttgggct tgaacgactt cgcgaagccg
      541 aacagctttt tgaagcgttc gtgcagctcg cggaagtcgg tgccttcaaa gactttggcg
      601 acgaagtggc cgccgttctt cagccagatt ttgctcaggt cgagcgccac atggaccagt
      661 tcgaccgagt tgccggcgtc ggcgtggtgc acgccggtcg tattcggagc catgtcgctc
      721 atgatcaggt cgaagccttt cggtgcgaac gcttgaatct gttcctgcat gaccgggtcg
      781 ttgatgtcgc cctcgagcag ggttgcgcct tcgagtggtt gaatcggcag cagatcgatg
      841 ccgatgacct tccccgtgcg tccggcctgt gccacggcat actgggtcca gcttcccggt
      901 gccgctccca ggtcgagaat gcggtttcca gagcggatca actggtactt ttcctgaatt
      961 tcctgcagtt tatagaccgc gcgcgaccgg taattctcgc gatgagcccg ccggaaaaac
     1021 ggatcctgca gacgattttt gaaccagccc ggattcggca tgaagagatg actctactcg
     1081 cctgtaatgg gtacgcacga caacttgaga ttgatcatgc gctgttcggc cggcggcccg
     1141 atgacctggt ctcgctcgat cttctcgacg atcagatcgc gaaccagttt tctggagttg
     1201 atctttcccg gccagttttt cagatacttg tacccgtcgc caccctgtga cagatagctg
     1261 ttgaaagcca ctttgtagac tcgcttcaga tcgagggggc tgtcgtcagc cagggtcagc
     1321 ttgacgatac gctgcatcgg cggccgctcc ggatcaaacg tgacgagaag attggatggc
     1381 acaagatagc cgaatgccgc catgaaatcg cgtctgagac cgctgatgtc gaggcgaaaa
     1441 tgcttgcggg catactcttt gtcggcggca tcagcctgct gaaacggtac cgaaagcccc
     1501 tcttccagaa tcttgacgat ctgcgcgccg gtcatgggca caacttccac caggttgttg
     1561 aatggctgca gctgaaaaat ctgatcccag atgaccggac cgctggcgat cggaaaccgg
     1621 accccgccaa cgttggtgag accgatctcc gcgccggtgc cctcgcgtat ggcatccgcg
     1681 acaaagttgc cttcgggcga gtcgcccccg atgaccccac gtaccagcgt cgcccggcag
     1741 gtgcctagat ggcgcttcac ctttgccgcg atgctcgaaa gcttttcgcc aatgaccgtc
     1801 tgcatccccg cgtcttcttc caagtgcgcc gtcgagagaa agtggggagt aaaggtcact
     1861 gcggggcgtt ctcccttgag caggtgcaga tcgacgcgcg aaacgtaccg ggtgtcatac
     1921 ccgggatgag cgatgggaac ccggttctta ccggcatatt ccagttctcg catttccgtg
     1981 tgactgtgcc cgccgagaat gagatcgatt ccctcgacgg tctccgcgat ctggcgatcc
     2041 tgctcgatgc caaggtgtga cagaagcacg aagacatcga caccctgcgt gcgcagtcgg
     2101 gaaatgagtt cgttcagaat gggcaccgga tcacgaaact cgagaccggc cacgttctcg
     2161 gccatcgaaa tgtccggcgt gcggggatcg agtgctccga tgatcgcaat ctgatggcct
     2221 ttgtagggta cacgaatgtg tgaacgcgcc gacgccatca ggcggccggt tgccgtttcg
     2281 aacacgttac agcacagaat cgggtgacgt gctgagcgca ggacatcctt gagtcgcgcc
     2341 agaccgtaat ccaaatcgtg gttgccgatg cccgccgcga gatagttcat tcggttcatc
     2401 aggtcgatca tgacctgacc ctgcgtctcc tcgacgatgg ccgttccctg aaagtagtcg
     2461 ccggcgtcaa gcagcagcgc tcgattgccc tgacgcagag cgtccagttt gattttctgt
     2521 acttgtgcct tcagaagcgg aaaaccagag acacgatgac cgctgccggt cgtatctgcg
     2581 gcgtcgatat cgctggcgat gtgaccgtgg gtgtcactgg tatgtaacag cgtcagcgtg
     2641 acacggtcgg c
//
LOCUS       DAORHD010000667         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_45976_length_2649_cov_3.342714, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000667 DAORHD010000000
VERSION     DAORHD010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45976_length_2649_cov_3.342714"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKO06_13745"
     CDS             <1..225
                     /locus_tag="PKO06_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70759.1"
                     /translation="SESVSPPTEIPTTSIKLPRDNPLEKAHKEYLEAYETYVQALREN
                     GPQTMETLNALADYQKKYQLYQMILRAQDK"
     gene            complement(299..820)
                     /gene="tpx"
                     /locus_tag="PKO06_13750"
     CDS             complement(299..820)
                     /gene="tpx"
                     /locus_tag="PKO06_13750"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003338685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HNV70760.1"
                     /translation="MAQESLKECGKVTFQGNPLTLLGNSVKVGDTAPDFTLLTTELKP
                     LSLKETKGVRILTTVPSLDTPVCDMQVRRFNQEAAKLPNVSIYAISADLPFAQKRWCG
                     GAGVDKLTTLSDHLSMGFAAAYGLYVKELRLVARAVMVIDSTGKVVYRELVPEITQQP
                     DYDGALKAAKEAK"
     gene            complement(896..1309)
                     /locus_tag="PKO06_13755"
     CDS             complement(896..1309)
                     /locus_tag="PKO06_13755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNV70761.1"
                     /translation="MIYLIITVILWGITPVIDKIAAVNADATVAIFVRGITIAVASTA
                     ILAASGKTALVTETSSKAILCLVVGGVLAGCLGVFTYMRAMQSVSDAGMVAVLTSTYP
                     LVALALSVALLNEKLSLAKGVGSVLIVIGIFLLNF"
     gene            complement(1344..>2649)
                     /locus_tag="PKO06_13760"
     CDS             complement(1344..>2649)
                     /locus_tag="PKO06_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV70762.1"
                     /translation="VFLEIALPQSEFVALAETVAGDLGFLALLAVVLALATGTYLAWH
                     IAQPITALAETTTRVAGGDFTNTPLAARSDEIGLLDRNFQDMIQSLKTERDLKESRMR
                     ELKTLFEISNAVNFLTDSEELLKFILSQSVEIFEAERGSIMLLDDQTDELVVKVATGG
                     RYRVLSSSPVKLGHGICGLAAREGTGKIANDGFRNREFRDFGSLVPVEDIQSLMCSPL
                     KFKEGTIGVINIVNRRNGGQFAESDLALLNLIASQAAVTIENNKLYELSITDGMTKLF
                     VHRYFLARISEELLRARRYSLRLSLIMIDIDNFKKFNDTYGHQTGDLVLQRVAQAIRD
                     TVRTGIDISCRYGGEEMAIILPETRSDEAFRTAERVRESIGQLQISHTSGTLRVTVSI
                     GVASYPGDAHDRDSLITAADKAMYYSKRSGKNCSTLSSQIPE"
BASE COUNT          637 a          752 c          682 g          578 t
ORIGIN      
        1 tcagagagtg tctctccacc aacggaaatt cccacgacat ctatcaaact gccccgggac
       61 aaccctctcg agaaagctca taaagagtat ctcgaagcat atgagacgta tgttcaagcc
      121 ctgcgcgaaa atggtcctca aaccatggag acgctgaacg ctctggcaga ctatcagaag
      181 aagtaccagc tgtaccagat gattttacga gcgcaggata agtaaatcgc ccccggtttc
      241 ccgagggcgt tcagcactga ttccccagat gacgcgactt caggtcgcag gtcgacggtt
      301 actttgcttc cttcgctgcc ttgagcgcgc catcatagtc gggttgctgc gtaatctcag
      361 gcaccaactc gcgatagacg actttccccg tcgaatcgat caccatgacc gcacgtgcca
      421 cgagacgcaa ttccttgacg tacaggccgt aagcggcggc gaagcccatc gacagatgat
      481 ctgaaagcgt ggtgagtttg tcgacgccgg caccaccaca ccagcgcttc tgagcaaatg
      541 gcagatctgc cgagatcgca taaatactca cgttcggcag cttcgccgct tcctgattga
      601 agcgccggac ctgcatgtca cagacgggcg tatcgagcga cggcacggta gtcaggatcc
      661 gtaccccttt tgtttctttc aatgacagcg gcttgagttc ggtcgtcaga agcgtaaagt
      721 cgggagcagt atccccgacc ttcacggaat tcccgagcag agtcagggga ttcccctgaa
      781 acgtgacttt tccacattcc ttcaaacttt cctgtgccat gagcggcatt cctccgttcc
      841 aaaagatgat tgcgatcacg atccataata tcacattttt catatcatat ctccttcaga
      901 aattcagtaa aaaaatgccg atgacgatga gaacagatcc gacccccttg gccagactca
      961 gtttttcatt gagcaaggcg actgaaagtg caagtgcaac cagcgggtag gtagaggtca
     1021 ataccgcgac cattccggca tccgagacgg actgcatcgc gcgcatgtag gtgaacaccc
     1081 cgagacagcc agcgagaact ccgccgacga caaggcaaag aatggctttg ctgcttgtct
     1141 cggtaaccag cgcggttttt ccgcttgcgg ctagaatcgc ggtgctggcc accgcaatcg
     1201 tgatcccacg cacgaagatg gcaaccgtgg catcggcatt caccgccgca atcttgtcga
     1261 tgaccggtgt gataccccag agaatgacgg tgatgatgag gtagatcatg cgagggctct
     1321 cctggatctt tcgaatgccg tgtctattcc ggaatctgcg acgaaagtgt cgaacagttt
     1381 ttcccggaac gtttgctgta atacatggcc ttgtcggccg ccgtgatcag tgaatctcga
     1441 tcatgtgcat cgccgggata cgaagcaacc ccaatactga ccgtcacccg taacgttcca
     1501 gacgtatggc tgatctgcag ttgcccaatc gactcacgaa cacgctcggc ggtgcggaat
     1561 gcctcatcgc tgcgtgtttc aggcaggata atggccattt cctcgccgcc gtatcgacac
     1621 gagatgtcga tgccggtccg aaccgtatcg cgaatggcct gagcgactct ctgcaacaca
     1681 aggtctccgg tctgatggcc gtaggtgtca ttgaacttct tgaagttatc aatgtcgatc
     1741 atgatgagag acaggcgcag agagtaacgg cgggcacgca gcagttcttc tgagatgcgc
     1801 gccaggaaat accgatgcac gaaaagcttg gtcatgccat ctgtaatcga aagctcatac
     1861 aacttgttgt tctcaatggt aacggctgcc tgcgaagcga tcagattcaa caaggccaga
     1921 tcgctttcgg caaactgtcc gccatttcga cggttcacga tattgatgac tccgatggta
     1981 ccttccttga atttgagtgg ggaacacatc aaactctgaa tatcttccac cggcacgaga
     2041 ctcccgaagt cccgaaactc gcgattacgg aagccgtcgt tggcgatttt gccagtacct
     2101 tcgcgggccg ccagaccgca gatgccatgc ccaagcttta ccggagagct cgataagacg
     2161 cggtatcgtc caccagtcgc aaccttgacc accagttcat ccgtctggtc atcgagcagc
     2221 atgatagatc cgcgctcggc ctcaaagatc tcgacggact ggctcaggat gaacttcagt
     2281 aactcttcac tgtcagtcag aaaattcacg gcgttgctga tctcaaaaag ggtcttgagt
     2341 tctcgcatcc gactttcctt cagatcccgt tcggtcttca ggctctgaat catgtcctga
     2401 aagtttcgat ccagaagccc aatctcgtcg cttcgagcgg caagaggcgt attggtgaag
     2461 tctccgccag cgacccgtgt ggtggtttcc gccaatgccg taatcggctg ggcaatatgc
     2521 caggccagat acgtcccggt cgccagtgcc aaaacgacag ccagcagggc cagaaagccg
     2581 agatcaccgg caacggtctc ggccaaggcg acgaactcgg attgcggcaa cgcgatttca
     2641 aggaacacg
//
LOCUS       DAORHD010000668         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46116_length_2644_cov_3.167632, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000668 DAORHD010000000
VERSION     DAORHD010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46116_length_2644_cov_3.167632"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="PKO06_13765"
     CDS             <1..406
                     /locus_tag="PKO06_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70763.1"
                     /translation="VLVKGPIVLGDVTRGLAGGRTLKKDLEKLNQNQLLTFVSLTGDK
                     ITLTGTRYYGVQIISLRPGLGRPVQQLGFDKGSGVAFMGGICLSTPVLESLTRQLSTS
                     PMIDYLATFGATSASYSVHIDEQVRDYEFSVQ"
     gene            387..860
                     /locus_tag="PKO06_13770"
     CDS             387..860
                     /locus_tag="PKO06_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70764.1"
                     /translation="MNSRCSKHGLSLLEITIAVVVLATAMLPILLLTTTTARDTYSME
                     KHLVAGQFATGVMDRLLALPYAECQRAVAGEDWPKSVAADSQMQPFLGAGSGFADALR
                     KSLREFTYEVALTEATGDEKGQMFTLQVIVKWPLDAAKKQMRQTMLQAVKFNENP"
     gene            860..1459
                     /locus_tag="PKO06_13775"
     CDS             860..1459
                     /locus_tag="PKO06_13775"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70765.1"
                     /translation="MMQHHERWRRQKARPAWSGRRALTLVEVLVVAVLGAIAMGGGML
                     LWSRTSQTVVKGDDMMDLQLILRSVQEQLRSDVRTMTALSSCAKHQVTFTSRLKGTDV
                     TITWTYQPAAKRLVRRVEGESERDFQAAGFVALAEFSPLVPQVTGSSSAPSAGASPAE
                     GIRLALELQSGKDPRTASRLAVVSQFTSRSIAPPFEIVR"
     gene            1718..1791
                     /locus_tag="PKO06_13780"
     tRNA            1718..1791
                     /locus_tag="PKO06_13780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Ala,seq:ggc)
     gene            1867..2076
                     /locus_tag="PKO06_13785"
     CDS             1867..2076
                     /locus_tag="PKO06_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoisovalerate dehydrogenase"
                     /protein_id="HNV70766.1"
                     /translation="MGNEILFLVQDSPEGGLEARALGYPIFTQAETLEELRMMVRDAV
                     HCHFEESLMPKIIRLHFVREEVLAA"
     gene            2073..2321
                     /locus_tag="PKO06_13790"
     CDS             2073..2321
                     /locus_tag="PKO06_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013034773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNV70767.1"
                     /translation="MKLPRDLSADELIKGLRRIGYEVTRQTGSHVRLTRATSPQHHIT
                     IPQHSPLKIGTLNSIVAELSDFLKLSKEELLKKIGIIS"
     gene            complement(2432..>2644)
                     /locus_tag="PKO06_13795"
     CDS             complement(2432..>2644)
                     /locus_tag="PKO06_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70768.1"
                     /translation="EKLRIDQWREKEATRDAVRQAIHDFLYSDVTGLPDAYSEAEIQS
                     KTESVFIHIFRAYPTLPSSLYEKAAS"
BASE COUNT          512 a          712 c          825 g          595 t
ORIGIN      
        1 ggtgctggtc aaggggccga ttgtcctcgg ggatgtcacg cggggtctgg ccgggggcag
       61 aacgctcaag aaagacctcg aaaagctgaa tcagaaccag ttgctgacat tcgtcagtct
      121 gacgggcgac aagatcacgc tgaccggcac gcgctactac ggcgttcaga tcatttcgct
      181 gcgccctggc ttggggcggc cggtgcagca gcttggcttc gacaaagggt cgggcgtcgc
      241 cttcatgggc gggatctgtc tcagtacgcc ggtgctcgag tctctgaccc ggcagctgtc
      301 gaccagtccg atgatcgact atctggcgac gttcggtgcg acgtcggcgt cctactcagt
      361 gcatatcgat gaacaggtgc gtgactatga attctcggtg cagtaaacac ggtctgtcac
      421 tgctggaaat caccatcgcg gtcgtggtgc tggcgacggc gatgctgccg attctgctgc
      481 tgacgaccac gacggcccgc gatacctatt ccatggaaaa gcatctggtg gcggggcagt
      541 ttgccaccgg agtcatggat cgtctgcttg ccctgccgta tgccgaatgc cagagggcgg
      601 ttgcggggga agactggccg aagagcgtcg cggcggattc ccagatgcag ccgtttcttg
      661 gcgcggggag cggttttgcg gatgccctgc gtaaaagtct gcgcgagttt acctacgaag
      721 ttgcactgac cgaggcgacg ggcgatgaga agggccagat gttcacgttg caggtcatcg
      781 tgaagtggcc tctcgatgcg gcgaagaagc agatgcggca gacgatgctg caggccgtga
      841 agttcaacga gaacccctga tgatgcaaca ccacgagaga tggcgacggc agaaggcgcg
      901 tccggcatgg tctggtcggc gtgccttgac gctggtcgag gtattggtgg tggcggtgct
      961 cggcgcgatc gcgatgggcg gcggcatgct gctctggagc cgcaccagcc agacggtcgt
     1021 caaaggcgac gacatgatgg atctgcagct gatactgcgt tcggtgcagg agcagttgcg
     1081 cagcgatgtg cgcaccatga ccgcactgtc atcgtgcgcc aagcatcagg tgaccttcac
     1141 gagtcgcctg aaaggaaccg atgtcaccat tacctggacc taccaaccgg ctgcaaaacg
     1201 cctcgtgcgc cgtgtggaag gggaaagcga acgcgatttt caggcggcgg gttttgtcgc
     1261 tctggccgag ttttcgccgt tggtgcctca ggtgaccggt tcatcttccg ccccttctgc
     1321 gggggcttcg ccggcagaag ggattcgtct cgcgctcgag ctgcagagtg gcaaagaccc
     1381 gcgcaccgcc tcacgtctgg ctgtcgtgag tcagttcacg tcgcgctcga tcgctccacc
     1441 gttcgaaatc gtgcgttgaa gccctgttgc cggcactgcg gattttgcct ctcatggctc
     1501 gcgccccctg tccgccgact caaaacgtcg gcagaaccgg gtgttcccat gacggttccg
     1561 gttcgagtag agacttgcac tccgaaatct cggagaccgg gtcggtagac gcgtagtgat
     1621 gaacgctgac gtcaagctga gtttcacgag ttgtacggaa ggtgcttttt tccacttgac
     1681 tttctggggc ttgtgacgta gaataaccct taccttcggg gctatggcgc agcttgggag
     1741 cgcgcttgaa tggcattcaa gaggtcgcgg gttcgatccc cgctagctcc accggtgaag
     1801 acccgctcag catcatgcag agcgggtctt ctgttattgt gagaacatcc tgtacggggg
     1861 gtgcttatgg gaaacgagat tctctttttg gttcaagatt cccctgaagg gggtcttgag
     1921 gctcgtgcct tggggtatcc catttttacg caagcggaaa ccctcgaaga actgcgcatg
     1981 atggtcagag atgccgtcca ctgccatttt gaagaaagcc tcatgccgaa aatcatccgt
     2041 cttcattttg tccgggaaga agttcttgcg gcatgaaact gccccgggat ctcagcgctg
     2101 atgaactcat taagggcctt cggcgtattg gttacgaagt gacgaggcaa accgggagtc
     2161 atgttcgtct gacccgcgcc acttcacctc agcatcacat cacgattccg caacattctc
     2221 cattgaaaat cgggaccctc aatagcattg ttgcggagtt gagtgatttt ctaaaactca
     2281 gcaaggaaga gctcctcaaa aaaatcggga tcatttcctg agagcaactc gttcagtgtg
     2341 aacctcatct tgggttcctc ccgcagtgca cgcgcttggc gattctttgt ctttgctatg
     2401 aagcgctttt ccgctcagaa tcgccgggtc actatgatgc ggctttttcg tacagcgacg
     2461 acggcagggt ggggtaggcg cgaaagatgt gaatgaagac ggactcggtt ttgctttgaa
     2521 tctctgcttc tgaataggcg tcgggcaggc cggtcacatc gctgtagaga aagtcgtgta
     2581 tcgcctggcg gacggcgtcg cgggtggctt ccttttcgcg ccactgatcg atgcgcagtt
     2641 tctc
//
LOCUS       DAORHD010000669         2644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46122_length_2644_cov_2.633835, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000669 DAORHD010000000
VERSION     DAORHD010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46122_length_2644_cov_2.633835"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PKO06_13800"
     CDS             <1..833
                     /locus_tag="PKO06_13800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV70769.1"
                     /translation="QHRIPEIGHDELGMLGQAFNQMLEDLRDLELARVLQERIYPSKP
                     LELANGWEIYGHCRSASEVGGDFYDYFLLPDGCCFLVVGDVSGHGTPSALIVAMAKGL
                     FSHPRNKTEPGQILHDVQVVFGRVLPRKRMMMTCFVGIFDPRSGLLRYGNAGHNYPYL
                     IHADGRLEPLRDKTMPLGVGKKSRNQTTDLQLADSDTLFLYTDGLVEALRPDQEMIGY
                     DAVQQQLSSLDILDAHSAVHQLRTWHDGQTAAGPQADDITLVFLKRHPMTRAEFTTRQ
                     "
     gene            complement(817..>2644)
                     /locus_tag="PKO06_13805"
     CDS             complement(817..>2644)
                     /locus_tag="PKO06_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70770.1"
                     /translation="QARETVDPVSRSADLLAVAESLASSGYLRTASDALAGCLYACSE
                     LDDGDTLRYRLERMVSFPAFSAIPWLRWYRTLQRVSNPYAAARLLLKASIRLHEAGRR
                     WIAGYAFDLAVKQYVLMVKMPQRLFTNEIQWVRKLISVAAAFLERSCPVQVPPIFDEL
                     SWTEIASEVLPTIPSCVHSRVVKEFVVTCMAKSKKAPTFTELATCTLIAGASQQQEAR
                     TFLISALSLLKEKSQVFPWIAELGWLWFCAGHTSDATAIDRILVALLPCAKDLGKPES
                     WSSLALLLHATGHQTVIKEWVVRQGLTSSRRPTPPFREHQSAVIQAITLHRLGFPEGH
                     MLAQQLHEHLAETSERWVLQSLMLLHAAFGNETKLASSMTAFVFKLAETSEDRDDFCR
                     PMSAALSEVLAYTEPGAEQLVLTTFDTVLKKVGSQRWSPDWIDHLFRETLFIVCIVNS
                     KSFFAVLRIIPSLLKITSTCDWSYVIIPDVLARTTSMNRLREALAIFDHFAAKGNELC
                     ADYHLEPVFHASCQAQDWELCRKIAERCPNQESSRGMLLLALAQAQRKRGNQAEADQL
                     IREALRCKRSASTHRVPFLLALSAASHNPLDPGNLQYLTAVS"
BASE COUNT          625 a          750 c          705 g          564 t
ORIGIN      
        1 tccagcaccg aattccggaa atcggtcacg acgagctcgg tatgctcggc caggcgttca
       61 atcagatgct cgaggatctt cgcgacctcg aactggcgcg cgtgctgcag gaacgcatct
      121 acccttcgaa gccgctcgaa ctggcgaacg gctgggaaat ctatgggcac tgccgctcag
      181 ccagcgaagt cggtggagat ttctatgact attttctgct ccccgacggg tgttgctttc
      241 tggtcgtagg agatgtttca gggcacggaa ccccctcggc gctgatcgtg gcgatggcga
      301 aaggcttgtt ttctcacccc cgaaacaaga ccgaacctgg ccagattctt catgatgtac
      361 aagtcgtctt cggtcgcgtg ttgccacgca agcgcatgat gatgacctgc tttgtcggaa
      421 tcttcgaccc tcgatcgggt ctgctgcgat acggcaacgc cggacacaac tacccgtatc
      481 tgattcacgc cgatggcaga cttgaacctt tacgggacaa gaccatgccc cttggcgtcg
      541 gaaaaaagtc acgcaaccag accaccgatc ttcagcttgc cgactcagac accctatttc
      601 tctacacgga cggtcttgtc gaagctctca gaccagatca ggaaatgatc ggctacgatg
      661 ccgttcagca gcaattgtcg tcactcgata tcctggacgc acactcagca gtacaccagc
      721 ttcgcacgtg gcacgacgga caaactgccg ccggccctca agctgatgac atcaccctgg
      781 tctttctgaa aagacatccg atgacacgtg ccgagttcac gacacggcag tgagatactg
      841 aagattaccc ggatcaaggg gattgtgcga tgcagcggag agagcgagga gaaacgggac
      901 ccgatgagtg gaagcacttc tcttgcacct caacgcctcg cgaatgagct gatcggcttc
      961 cgcctgattt ccgcgttttc gctgtgcctg agccagtgcg agaagaagca tcccgcgtga
     1021 tgattcctga ttcgggcagc gctccgcgat cttcctgcag agctcccagt cctgtgcttg
     1081 gcagcttgcg tggaacacgg gttccagatg atagtctgca cacagctcgt tcccctttgc
     1141 ggcaaagtgg tcgaagatgg caagggcctc tcgcagtcta ttcatgctcg ttgtcctggc
     1201 caacacatca ggaataatga cgtaactcca gtcgcaggtt gaggtgattt tcagcagact
     1261 cggaatgatc cgcagaacgg cgaaaaagct cttcgagttt acgatgcaaa caatgaagag
     1321 ggtctctcga aacagatgat caatccaatc tggagaccag cgttgtgacc ccaccttctt
     1381 caaaacggtg tcaaacgttg tgagaacgag ttgttctgcg cccggttccg tatacgcaag
     1441 cacctcactg agtgctgcgc tcattggacg gcagaagtcg tcccggtctt cagaggtttc
     1501 cgccagttta aatacaaagg cggtcatgga gctcgccaat ttcgtttcgt tgccaaaagc
     1561 cgcatgcaag agcattaagc tctgtagaac ccatcgttcc gaggtttcag ccaaatgctc
     1621 gtgtagctgc tgagcgagca tgtgaccttc tggaaacccg agtcgatgaa gtgttattgc
     1681 ctgaatgact gcggactgat gctcacggaa cggaggtgtc ggacgccgcg atgaggtcaa
     1741 cccttgtcgc acgacccact ccttgatgac cgtttggtga cccgtcgcat gaagtaacag
     1801 agcgagagag ctccaggact cgggtttgcc caggtctttg gcgcacggaa gcagcgcaac
     1861 caggattctg tcaatcgctg tggcatcaga cgtgtgaccg gcacagaacc aaagccagcc
     1921 taactccgca atccatggaa acacctgaga tttctctttc agtaatgaga gcgctgagat
     1981 caggaaagta cgagcttcct gctgctgaga tgccccagcg atcaatgtgc atgtcgctaa
     2041 ctctgtgaaa gtcggtgctt tctttgactt tgccatgcag gtcacaacga actctttcac
     2101 gacacgagaa tgaacgcatg atgggatggt cggcagaacc tctgaggcaa tctccgtcca
     2161 tgacagctcg tcaaaaatgg gagggacttg taccggacac gatctctcga gaaatgccgc
     2221 ggcaaccgaa atcagcttcc gcacccattg gatctcattc gtgaacagcc gttgaggcat
     2281 cttcaccatc aagacgtatt gcttgacggc aagatcaaag gcatagccag cgatccaccg
     2341 tctacccgct tcatgcagtc gaatcgaagc cttcagaagc agtctggcag cagcatacgg
     2401 atttgagacc cgctgcagcg ttcgatacca gcgcagccat ggaatggccg agaaagccgg
     2461 gaagctcacc attcgctcta accgatagcg cagggtatcg ccatcatcca gttcagaaca
     2521 cgcgtacaga catccggcga gcgcgtcact cgccgtcctc agatagcctg aagaggcgag
     2581 tgactccgca actgccagca gatctgccga acgagaaacc gggtccactg tctctcgcgc
     2641 ctgt
//
LOCUS       DAORHD010000670         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46193_length_2642_cov_2.666409, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000670 DAORHD010000000
VERSION     DAORHD010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46193_length_2642_cov_2.666409"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PKO06_13810"
     CDS             complement(<1..614)
                     /locus_tag="PKO06_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70771.1"
                     /translation="MRITSSGSLFTLAVCCWLTGWPVLAETSAVADSPPAKSEAAKTP
                     GADRASTGDDAASQTDISARITRTEEMIRKIDQEVPGMLKVSGWPTLTGYFQNGKLAK
                     AIMDEETYWYNAQGLCFAYTSTDNFPELGPDVRIRCRFFQDDVDAVFAMGREDHGYKM
                     EPYERSAFEDGDAYPPGTAKQRLDYLLSLDPKGIPLPTTWTMNNP"
     gene            1032..1592
                     /locus_tag="PKO06_13815"
     CDS             1032..1592
                     /locus_tag="PKO06_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70772.1"
                     /translation="MRRALSLSEFAARLQVSARAVWQLFLVFPRFLHGEFEHGPFLLL
                     NPATQDRVVTILDLKRKGWSEAEVRSWLERTVDLSMPMPEHASAPSGVRGTAPSSTGR
                     PGTVQSNQVNTASEPALKSAAPVSSRRASVQPPVPGAGLAEENLRGSVEEELLVWKRL
                     VDLAIQPFLGRIDALARDVRTLRRNG"
     gene            1644..>2642
                     /locus_tag="PKO06_13820"
     CDS             1644..>2642
                     /locus_tag="PKO06_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70773.1"
                     /translation="MESINTSSFVQTSASGDTPSAEPAAGTGLRWLWGLFLALLVLPA
                     LFFLSILRDVARDEEQRQLRELVHRADRLLTGIERELPPLTALNAFAARLIRDVPVRQ
                     CDETIRNRLEQLIRRRLHTSVQLLWFDSQGRAMNPGAPGFGGGQTVWAAFWRLLQNLD
                     TVTDRDRFLARNLVLSYMGRIMPTEILLTAVDAVISLYFSGEPSYFTLLRFRDEKGVV
                     QISCFLILNRNQLPDDWYIRRVLRRVRHSTLQAGAWRFSRQQEITPSALEPNVLPVLA
                     SRLLLGEFESVIGEDLYVSRISGADPDLMLSVRVTGGSRVTLSGKLLRWWFPMLLLL"
BASE COUNT          487 a          748 c          810 g          597 t
ORIGIN      
        1 gggttgttca tcgtccaggt tgtcggcaga ggaattcctt tcgggtcaag gctgagcagg
       61 taatcgagac gttgcttggc tgttccaggg ggatacgcat cgccatcctc gaaggccgag
      121 cgttcgtagg gctccatctt gtatccgtga tcttcacggc ccatcgcaaa gacggcatcg
      181 acatcgtcct gaaagaaccg gcacctgatc cggacatccg gacccagctc gggaaagttg
      241 tccgtgctgg tgtaggcaaa acagagccct tgagcattgt accagtaggt ttcttcatcc
      301 atgatggctt tggctagctt gccgttctga aagtaccccg tcagggtcgg ccatccgctc
      361 actttcagca tgccgggcac ttcctgatcg atctttcgaa tcatctcttc ggtgcgggtg
      421 atcctggcac tgatgtcggt ttgggaggcg gcgtcatcgc ccgttgaggc gcgatcagct
      481 cccggagttt tcgccgcttc cgacttcgcg ggcggggaat cggcaacagc gcttgtctca
      541 gccaggaccg gccagccggt taaccaacaa cagaccgcaa gagtgaacag cgaacctgaa
      601 gaagtgatgc gcatgattat gcctccggca aaagagctcg aaaatgaaca tggattcgaa
      661 ttccctgtat atcatattgc cgctcggtct tcacctgctg cgacacgagt cgggcaatgg
      721 cgaaccagag ctcgaaccag gcacggtttg tggctgagct ccagacaatg ctcgccgatc
      781 agccagtgcc tggatacagc gaaattgccg cacaatgtgc ccgaaagaga aagcggctgg
      841 ccgctcggtg acgagggccg gccgttgtat aaccggtacg gaagattcag tgccgcgcag
      901 agtattgatg caggcttccg agcggccgtc ctgggcagtg gggccgtcat cgaaggtcag
      961 aacgatctct tcagtttggt cggtgttctg ccgatggtga gggtgtatta tcctctgccc
     1021 ggaggtgcat tatgcgcagg gcgctcagtc tgtcagagtt cgcggcgcgg ctgcaggtgt
     1081 cagcccgtgc ggtgtggcag ttgtttctcg tctttcccag gtttctgcac ggggaattcg
     1141 aacatgggcc gtttctgctc ctgaacccgg ccacccagga tcgcgtcgtg accattctcg
     1201 atctgaaacg caaaggctgg agtgaagcag aggttcgcag ctggcttgag cgcacggttg
     1261 atctctcgat gcccatgcca gagcatgcga gtgcgccttc aggagttcgg ggaactgctc
     1321 cgtccagtac cggccggcca ggtaccgttc aatcgaacca ggtgaacact gcgtctgaac
     1381 ccgctctgaa atcggcagcg ccggtctcca gcagacgcgc ttccgtgcaa cccccggttc
     1441 cgggtgcagg gttagccgaa gaaaacctcc gagggagtgt cgaagaggag cttctcgtct
     1501 ggaagaggtt ggttgatctc gccattcagc cgtttctcgg acggattgac gccctcgcac
     1561 gcgatgtgcg aacgttgcgg cgcaacggtt gagacgtttt cgcccgatgc gtttcatgaa
     1621 aaattgcgct accatgaaac ggcatggaga gcatcaacac cagcagcttt gtccaaacca
     1681 gcgcatccgg ggatacccct tccgctgaac cagccgcggg aactggactg agatggctat
     1741 ggggcctgtt tctggccctg ctcgtgcttc cagccttgtt cttcctgtcg atactccgtg
     1801 atgttgctcg ggatgaggag cagaggcagc tccgtgagtt ggttcaccgt gccgaccgtc
     1861 tgctgacggg tatcgaacgg gagttgcccc cgctgaccgc tctgaacgcg tttgccgcaa
     1921 gattgattcg cgacgttcct gtgcgacagt gcgacgagac gatacgaaac agactggaac
     1981 agctgattcg ccgccgcctg catacatcgg tgcaactgct ctggtttgac tctcaggggc
     2041 gagcgatgaa ccccggcgct ccggggttcg gcggcgggca gacggtctgg gcggcgttct
     2101 ggagactgct tcagaatctc gacacggtga ccgaccggga tcgctttctg gcccgcaatc
     2161 tggtgctttc ctacatggga cgcatcatgc cgaccgaaat tctgctcacg gctgttgatg
     2221 ccgttatctc gctgtacttc agcggtgagc cgagttactt cacgctgctg cgctttcgtg
     2281 acgagaaggg cgttgttcag atctcgtgct tcctcattct gaaccgcaat cagcttccgg
     2341 acgactggta cattcgccgg gttttgcggc gcgtgcggca ttcgaccctt caggctggtg
     2401 cctggcggtt ttcacgtcag caggaaatca ccccctctgc gctcgaaccg aacgttctgc
     2461 cggttctcgc ttcccgtctg ctgctcggag agttcgagag cgtgatcgga gaggacttgt
     2521 acgtgtcgcg aatctcggga gcggaccctg atctgatgct ctcggttcgc gtcacgggcg
     2581 gtagtcgggt gacgctttcc ggaaagttgc tgcggtggtg gttcccgatg ttgctgctgc
     2641 tc
//
LOCUS       DAORHD010000671         2640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46237_length_2640_cov_3.222437, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000671 DAORHD010000000
VERSION     DAORHD010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46237_length_2640_cov_3.222437"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..679)
                     /locus_tag="PKO06_13825"
     CDS             complement(50..679)
                     /locus_tag="PKO06_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV70774.1"
                     /translation="MNTLIFQHSRASFTRTSSRYRSTHGFTLIEIVTAVLLGSILFLA
                     LTQLLSSGMRVSQKGSSHLTNMQAAAILLSQIESDLALAVELDTGATSAPGAADTPVE
                     VFSIKIAVSPSNVATPHTLLLKYQPSESRLGFERRQADAEPYRFCAGLQTQVTLRRVV
                     VPTTGHRGALIEVRVKTPPLGTEETVVARFVPCHNLPANRALAAPGWTW"
     gene            complement(676..1104)
                     /locus_tag="PKO06_13830"
     CDS             complement(676..1104)
                     /locus_tag="PKO06_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70775.1"
                     /translation="MRGPNGFSFTEILVTVLVVALGIIPVFLVFSKGSMGTILSRDEM
                     TAHIYAAELIDWCQTHRYDQLAECENHSVPELSLGGDCTSRIDDKYRRQLTVKALATP
                     GGAGNWPMQYKVLTATIHWNSQGVERTFVMTGLLSRGRTP"
     gene            complement(1104..>2640)
                     /locus_tag="PKO06_13835"
     CDS             complement(1104..>2640)
                     /locus_tag="PKO06_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70776.1"
                     /translation="LVLDHDGPAAVPTTSAGPSGAAIPTTFQEFVTTPRGLVYLGGPG
                     QILLNLARTDKTKFGTANSGESFQTFRNSRGEGFTPRHVGTDNLSHQVVVSEMECGSS
                     NSPVPINKSYYDVVNSGAFGRAMISIYHMEYNSIFRLMGVECDRSPTIVLGNVMRHSL
                     LVRKMDVMLPPPARLFRVLPCLDYPVYQSKIGFEFLPLNEAFGLAAVYTPDYLRYLIE
                     FGSAVASRPYNIGLAFCRDQANYLAFAAFPPGDRLRRLMDSDPCAGESDQHDLPDPWK
                     AALTAGSAPGSSDSPGASSEATGPAAGVTSLRTMAPFTDLLMNTNRESLTLPAAGETG
                     TALDLLRRRGLLVGSKLLLNGWVRLKPPLGQLVFDQPLEVLGNGGLILEKGDIVIRAS
                     LQRSPSAPERRAAVLTLATLDGNIILETPTGTNVHASLFAFAPDGSAAAGHVRFQAPV
                     ALDGSVAARGLFASTAEIARFPGGRLQYNPLLAALPNSSEEATSEKPTLTFAFEPLPW
                     SLD"
BASE COUNT          529 a          754 c          880 g          477 t
ORIGIN      
        1 tgccttccgt cagctcctgg gaatcaatcg ctgagcggct tctctgtgat caccacgtcc
       61 agccgggggc ggcgagggcg cggttggcgg ggaggttgtg gcaggggaca aaacgcgcga
      121 cgacggtttc ttcggtgccc agcggcggag ttttaacgcg cacctcgatc agggcgcctc
      181 ggtgaccggt ggtcggaacc accacccggc gcagtgttac ctgtgtctgc agaccggcgc
      241 agaaacgata gggttctgca tctgcctgtc ggcgttcgaa gccgagtcga ctctcggacg
      301 gctggtactt gagcagcagc gtgtgcggtg tggcgacgtt cgaagggctc acggcgatct
      361 tgatggaaaa gacctcgacg ggggtatcgg ctgcgcctgg agcgcttgtt gccccggtat
      421 ccagctcgac ggcgagggcc agatcggact cgatctgact cagcaggatc gccgcggcct
      481 gcatgttggt gaggtgactc gaacccttct gactcacccg catcccgctc gacaacagtt
      541 gtgtcagggc aagaaacaga atactgccga gcagaaccgc cgtgacgatc tcgatcagcg
      601 tgaacccgtg cgtcgatcga taccgggaag atgtccgagt gaatgaggcg cgagaatgct
      661 gaaatatcaa ggtattcacg gggttcgacc tcgactgagc agacccgtca tgacgaacgt
      721 gcgttccacg ccctggctgt tccagtgaat ggtcgccgtg agaaccttgt actgcattgg
      781 ccagtttccc gcaccaccag gggtagcaag tgcctttacc gtcaattgcc gccggtattt
      841 gtcgtcgata cgactggtac aatcgccccc gagcgacagt tcgggcacgg agtgattctc
      901 gcactcggcc agctgatcgt accggtgagt ctgacaccag tcgatgagtt cggcagcata
      961 gatgtgcgcc gtcatttcat cccgcgagag aatcgttccc atcgacccct tcgaaaagac
     1021 cagaaacacg ggaatgatgc ccagtgcgac aacgagtacg gtcacgagga tctcggtgaa
     1081 ggaaaatccg ttgggtccgc gcatcagtcg agactccagg gcaggggctc gaacgcaaac
     1141 gtgagggtgg gtttttcgga ggtcgcttct tctgatgaat tgggaagcgc ggcaagcagc
     1201 gggttgtact gcagtcgccc acccgggaat cgggcgatct ctgccgtcga agcgaacagg
     1261 ccgcgggcag ccaccgaacc atcgagggcg accggtgcct gaaatcgcac gtgtccggcc
     1321 gctgccgaac cgtcgggagc gaaggcgaac agggacgcat gaacgtttgt acccgtcggt
     1381 gtttcgagaa tgatgttccc gtcgagggtg gcgagagtga gaacagctgc ccggcgttcc
     1441 ggggcactgg gcgaacgctg aagtgaggcg cgaatcacga tatcgccctt ttccaggatg
     1501 agaccgccgt tgcccagcac ctcgagcggc tggtcgaaaa cgagctgtcc gagaggcggc
     1561 ttgaggcgca cccacccgtt cagcaacagc tttgaaccca ccagcagacc acgtcggcgc
     1621 agaaggtcga gcgcggttcc cgtctcgccc gcggcgggca gggtcagact ctcgcggttg
     1681 gtgttcatca gcagatcggt gaacggagcc atcgtgcgca ggctggtcac acccgcggcg
     1741 gggccggttg cttcgctcga agctccggga gagtcggagg aaccgggggc ggatcctgcc
     1801 gtcagagcgg ctttccatgg gtcgggcagg tcgtgctggt ctgactcgcc ggcgcagggg
     1861 tccgagtcca tgagacgtct cagcctgtct ccgggcggga aagcggcgaa cgccagataa
     1921 ttcgcctggt cgcgacagaa ggcgagaccg atgttgtacg gccgagacgc gacggccgaa
     1981 ccgaactcga tgaggtagcg aagataatcg ggggtgtaga cggcggccag gccgaacgcc
     2041 tcgttgagcg gcagaaactc gaacccgatc ttcgattgat acaccgggta gtcgaggcac
     2101 ggaagcaccc ggaagaggcg ggcaggtggc ggcagcatga catccatctt gcgcaccagc
     2161 agagagtgac gcatgacatt tcccagaaca atggtaggtg aacgatcgca ttcgacgccc
     2221 atcaaacgaa atatcgagtt gtactccatg tggtagatgc tgatcatggc gcggccgaag
     2281 gcacccgagt tgacgacgtc gtagtagctc ttgttgatcg gcaccggact gttcgaagat
     2341 ccgcactcca tttcggaaac cacgacctga tgcgagaggt tgtcggtgcc gacgtgacgc
     2401 ggggtgaacc cttcgccccg actgttgcga aaggtctgaa aactttcgcc ggagttggcc
     2461 gtgccgaact tcgtcttgtc ggtgcgagcc aggttgagca gaatctgccc cgggccgccg
     2521 aggtaaacca ggccgcgcgg tgtggtgacg aattcctgaa acgtcgtggg aatggcggcc
     2581 ccgctcggcc cggcgctggt cgtcggcacg gctgccgggc catcgtgatc gagaaccagc
//
LOCUS       DAORHD010000672         2637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46316_length_2637_cov_2.733540, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000672 DAORHD010000000
VERSION     DAORHD010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46316_length_2637_cov_2.733540"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..2261
                     /locus_tag="PKO06_13840"
     CDS             228..2261
                     /locus_tag="PKO06_13840"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HNV70777.1"
                     /translation="MPVTVITALWAIVVAASYLSWHPLSFPLEGFRLPTGTAVAHLWP
                     GIVAVGLCWFSGHGLLILLGCPPQTLLTAVTALPTGLMVTAICGLLFGSTIGFTPASL
                     GLTILSLLAISAVSCTRTPWKHVFEEFSRLCSPIGIRITLALLILGTALNLPGVVAPD
                     LAYDDQMYHLSIPRLYRELGRLSFLGDLFPANRPQSFHLFMTWIGLVGNDSAMRALNW
                     LLPGMIALCLLLTLGKRFNATAGCCAALALVMHPQLAWLSRTAYTDLIVLYLALAVVF
                     ALSELHRHSSPHVQPHLICLALLSGYAAQVKTSGALLVIISIGGAAATLHWHRLAGEK
                     RQTSCHLFLLFAAAVIILSSPWYLRNWLSVGNPFFPYLQSVFAGQLYLPDYHHIFSEL
                     FQCDMPFLYNHELLLDRFGMGQDILSMLLLPWNVTIYGRLHDPAVSLRFFDGQIGFAP
                     LALAPLLIFQVTRQQVSAGFRLLMATVFLSGIFWGFGSHQIRFLLPTLGFVSIAIGIL
                     AGTNDAMRRLTIAVLIAGLPTAIGFAVNRNVFLSDFTRGDKTRDEFLKEQLPFMGSYQ
                     FINRETASQTRVLPLFEERIYYLNRPFTWMPLVPYPFISAFIVSKSADQVRQYLDRCH
                     IDLIYLPRNGFQTLRDIIDDAAYKQRIIEFFASPSRRLYADRFGEVWTYRQAD"
     gene            complement(2279..>2637)
                     /locus_tag="PKO06_13845"
     CDS             complement(2279..>2637)
                     /locus_tag="PKO06_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70778.1"
                     /translation="LFEKEGASARAYGMLNRVVSQAPHWMDARMDLYRVAKKLGQTAE
                     GERLASESLQVLAAAWREPTSLSWRASEYRPTGDNWTVLLSQVVLRRLINRLSPGFVP
                     YEERKLAWKAFWSENR"
BASE COUNT          489 a          802 c          635 g          711 t
ORIGIN      
        1 tattctctcc tctgacggtt gaagatatgg ttcgcttcat gtactttctc tgggaagccg
       61 aagacaaccc catgtgctcc tcaatgtccg agagagacac gccagacgga accgggtcat
      121 gagccgacac gcagaagcca acgagtgaag tttccgatca catgctagaa tctcacgcag
      181 caacgggccc aaagtcagca gagagccagg aaaaacagca cctcgtcgtg ccagtcaccg
      241 ttatcactgc gctgtgggca atcgtggtgg ctgcttcgta tctatcttgg catcccctgt
      301 cctttccgct tgagggcttt cgcctgccga ccggtacggc agtcgctcac ctgtggccag
      361 gaatcgtggc tgttggtctg tgctggttct cgggtcacgg tctgctgatt ctgctcgggt
      421 gcccaccgca aactctctta actgccgtga ccgcattgcc cacaggcctc atggtcactg
      481 ctatctgcgg acttcttttt ggttccacca ttggcttcac accagccagc ctgggattga
      541 ccatcttgtc gctgctcgcc atttccgcgg tcagctgtac cagaaccccc tggaagcacg
      601 tgttcgaaga attttcccgc ctctgttccc cgatcggcat ccgtatcact ctggcgctgc
      661 tcattctggg aaccgctttg aatcttcctg gggtcgtcgc tcctgacctt gcctatgacg
      721 atcagatgta tcatttgtcc attccgcgac tgtatcggga acttggtcgc ctgagctttc
      781 tcggcgatct gtttccggcg aatcgcccgc aaagttttca cttgttcatg acctggatag
      841 gcctcgtcgg caatgattca gcgatgcgcg ctctgaactg gcttttaccc ggtatgatcg
      901 ctctctgcct gctgctgact ctcgggaagc ggttcaatgc cacagccggt tgctgtgcgg
      961 ctctggctct ggtcatgcat ccacagcttg cctggttgtc tcgcaccgcc tataccgatc
     1021 tcatcgtgct ctatcttgcg cttgccgttg tgttcgcact gtctgagtta caccggcatt
     1081 cctcgccgca cgttcaaccc cacctgatct gtcttgccct cctctccgga tacgcggctc
     1141 aagtgaaaac ctccggagcc ctgttggtga tcatctcgat tggcggtgcc gcggcaacac
     1201 ttcattggca tcgacttgca ggtgaaaaac ggcaaacatc atgtcacctc tttctgcttt
     1261 tcgcagccgc ggtcatcatc ctctcaagcc cctggtatct acgaaactgg ctttcagtcg
     1321 gcaatccctt ctttccgtat ctacagtccg tgttcgctgg ccagctttac cttccggact
     1381 accatcacat cttttcggag ttgttccagt gcgacatgcc gttcttgtat aaccacgagc
     1441 tgctgctcga tagattcggc atgggtcaag acatcctttc gatgctgctg ctcccctgga
     1501 acgtaactat ctatggacga ctgcatgatc cggctgtctc tcttcgtttt tttgacggcc
     1561 agatcggctt tgcccctctt gccctcgcac cgcttttgat ttttcaggtc accaggcaac
     1621 aagtcagcgc cgggtttcgt ttgctcatgg cgacagtatt tctttcggga attttctggg
     1681 gctttggttc tcatcagatc agatttcttc ttcccacact cggcttcgtt tcaattgcaa
     1741 ttggaatcct agctgggaca aatgacgcta tgcgtcgact gaccatcgcc gttctcatcg
     1801 ctggcttgcc gactgccatt ggcttcgcgg tgaaccgtaa tgtctttctt tccgatttca
     1861 cgcgtgggga caagacacgg gacgaatttc tgaaagagca acttccgttc atgggttcgt
     1921 accagtttat caatcgcgaa actgcctctc agacgagagt gttaccattg ttcgaagaga
     1981 gaatttacta tcttaaccgt ccgttcacct ggatgccgct cgttccgtac ccgttcattt
     2041 cggcgttcat cgtctccaag agcgctgatc aggtgagaca gtatctcgat cgatgccaca
     2101 tcgatctcat ctatcttccc agaaacggtt ttcaaacctt gagagatatc attgatgacg
     2161 cggcgtacaa acagcgaatc atcgagtttt ttgcctcgcc atcacgtcgt ctttacgctg
     2221 accggttcgg cgaggtctgg acataccgcc aggcagacta gctctacgga gcccgttgtc
     2281 agcgattttc gctccagaat gctttccacg cgagttttcg ttcttcatac ggaacgaacc
     2341 ctggtgacag acgattgatc aacctgcgca gaaccacctg gctcagcagg accgtccagt
     2401 tgtcgcccgt ggggcgatat tcagacgcac gccacgagag actggtcggc tctcgccatg
     2461 ctgctgccaa cacctgcagt gattcagacg ccagccgttc gccctctgcc gtctggccca
     2521 gttttttcgc aacccggtac aagtccatac gcgcatccat ccagtgcggc gcctggctta
     2581 cgacacggtt cagcatgcca tacgcccgtg cacttgctcc ctctttctcg aaaagca
//
LOCUS       DAORHD010000673         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46333_length_2636_cov_3.547850, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000673 DAORHD010000000
VERSION     DAORHD010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46333_length_2636_cov_3.547850"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2097
                     /locus_tag="PKO06_13850"
     CDS             <1..2097
                     /locus_tag="PKO06_13850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV70779.1"
                     /translation="VDVDLASLGTTQDEPAVTMLTRENLDQTLDLLGSNVDRPVSAVG
                     RDVEMVIRDFPFPPGYTAQLRGTPADMADAGERRAVALKRGMIFLVLVLILMFETVWH
                     PLLIVASIPLALIWGMWGLVLFDKPMCVPALSGFILLGGTIVNNAIILLDFIEQARVQ
                     GCEKRQALLDSVKVRLRPIFVTTFSTVLGLLPLTFEQAIGLERLSPLGVVASCGLFGG
                     TLMTMIVIPVLYDVVTDVGAWLRHCGRLMSLRPAGSTMVLVIAILASTVTPILAQTEN
                     SNSLPVSAANVPSSETDRCWSVRDCLSRALTQSPMLSILVAEKRAARGAKEEVAASWR
                     PQIETAGSIRHWDRRRVGMLGIAPTERQFYDRDLSEFRLTIRQLLWDGGQIGDRLQAA
                     SLLAQSKGLQIERARAEIQADVWSIALSIFTTEALLEASEKALTDIRATLTKMQDMVS
                     VGKVPHVDVLRVQSRVQEVLETQEVYRQARANHLAHLSTVMGETTAIASLSRDGLPEQ
                     SFEEDADVELLVQKAMNRRADVEAARLATHGAKKQVQAQQKGTRPQLFLNATANRYGD
                     GSGWGRELGFVGAEVQWTLDDGGRTRARVRQARAQADQATERERLAELKVAEQVRVAA
                     ANVRSAKARCLRNQANLRLAAEAFRIEQLKYEQGKGTINDVLDAEAAKFFAEAQVIRS
                     HNEALAAQIALETSVR"
     gene            2198..>2636
                     /locus_tag="PKO06_13855"
     CDS             2198..>2636
                     /locus_tag="PKO06_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HNV70780.1"
                     /translation="MEVQLLFPGSYCMAKLALTNGESIRAESGAMVAMSDTVDVEGKA
                     EGGVMGMFKRAFSGESLFLQTLKAARGAGEVFVSPSEPGDVQLLELNGATEYFIQKGG
                     FLAAEDSVTMETVSQGFFKGFMSGEGMFIQKASGKGKLLISSFG"
BASE COUNT          531 a          695 c          840 g          570 t
ORIGIN      
        1 gtagatgtcg atctggccag tctgggcacg acccaagatg aaccggcagt gacgatgctg
       61 acccgggaaa atctcgacca gactctcgat ctgctgggct cgaatgtcga ccgccccgtt
      121 tccgctgtcg gtcgggatgt tgagatggtc atccgcgatt ttccgtttcc tccggggtat
      181 accgcgcaac tgcgcggaac tcccgcggac atggctgatg ccggtgagcg tcgggcggtg
      241 gccctgaaac gcggcatgat ttttctggtg ctcgtgttga ttctgatgtt cgaaaccgtc
      301 tggcatccgc tattgattgt tgcgtcgatc cctctggcgc tgatatgggg aatgtgggga
      361 cttgtactct tcgacaaacc gatgtgcgtg ccggcgctga gcggcttcat tctgctgggc
      421 ggcacgatcg tcaacaacgc gatcattctg ctcgacttca tcgaacaggc cagggtgcaa
      481 ggctgcgaga aacgtcaggc actgttggat agcgtaaagg tacgtttgcg cccgatcttc
      541 gtcacgacct tctcgaccgt gctcggtctg ctgccgctga cattcgagca ggccatcggc
      601 ctggaacgcc tttccccgct tggggtggtg gcaagttgcg gactgtttgg aggaactctg
      661 atgaccatga ttgtcattcc ggtattgtac gatgtggtga ccgatgtcgg cgcgtggctg
      721 agacactgtg gtcggttgat gtcgctgcgc ccagcaggct cgacgatggt tctggtgatc
      781 gcgattctgg cttcgaccgt cacgccgatc cttgcacaga ccgagaatag taactcgctg
      841 cctgtgagcg cggcgaatgt cccgtcgtcg gaaaccgacc gttgctggag cgttcgtgat
      901 tgtttgagcc gagccctgac ccagtctccg atgctgtcca tccttgtcgc cgaaaagagg
      961 gccgcccgcg gcgcgaaaga ggaagtggcc gcgtcttggc gacctcagat agagacggcg
     1021 ggaagcattc ggcactggga tcggcgccgc gtgggtatgc tcggtattgc cccgaccgag
     1081 cggcagttct atgaccgtga tctttccgag tttcgtttga cgatccgtca gttgctgtgg
     1141 gacggcggcc agatcgggga tcgtctgcag gcggcttctc tgctggcaca gagcaaggga
     1201 ctgcagatcg agcgggcacg cgccgagatt caggctgacg tctggtcgat cgcgcttagc
     1261 atcttcacga cggaagcgct gctcgaagct tcggaaaaag cgctcaccga cattcgagcc
     1321 acgcttacga agatgcagga tatggtatcc gtcggcaagg taccgcatgt cgacgtgctg
     1381 cgcgttcagt cgcgggtgca ggaggttctg gaaacacagg aagtctatcg gcaggcgcga
     1441 gccaaccacc ttgcacatct ctcgaccgtc atgggcgaga cgacggcaat cgcctcgctc
     1501 tcgagagatg gcttgccaga acagtctttt gaagaagatg ctgatgttga actacttgta
     1561 caaaaagcca tgaatagacg agccgacgtc gaagccgctc gtctcgcaac tcatggtgcg
     1621 aaaaaacagg tacaggctca acaaaaagga acgcgtccac aactgtttct caatgcgacc
     1681 gcgaatcgct atggcgatgg gagcggttgg ggtcgtgaac tcgggtttgt cggggcagag
     1741 gtgcagtgga ccctcgatga tggaggccga acccgggcgc gagtacggca ggcgagagcc
     1801 caggcagatc aggcgactga acgagagcga ttggccgaac tgaaagttgc cgaacaggtg
     1861 cgtgtggctg ccgcaaatgt gcgttcagcg aaagcgaggt gtttgcgaaa ccaggccaat
     1921 ctccgtctgg ctgccgaagc ctttcgtatt gaacaactca agtatgaaca gggcaaaggc
     1981 accatcaatg atgttctgga tgccgaagcc gcgaagtttt tcgccgaagc gcaggtgatc
     2041 cgctcacaca acgaggctct agccgcgcag atcgctttgg agacgtcggt tcgctgatgc
     2101 ccttaccatt ttcttcacca tagaaaaatg gggccatacc gagtaacatt ggggtgtggt
     2161 cgatccgatc acgatcaatt tatcgggagg atgagacgtg gaagttcaac tgctgtttcc
     2221 gggttcgtat tgtatggcga agttggcact gacgaacggc gagtcgattc gtgccgagtc
     2281 gggggcgatg gtggcgatga gcgataccgt cgatgtcgag ggcaaagccg agggcggcgt
     2341 gatgggcatg ttcaaacgcg cgttctcagg ggaaagcctg tttctgcaga ccctgaaagc
     2401 cgctcgcggc gcgggtgaag tgttcgtctc cccctctgag ccgggtgacg tgcagctgct
     2461 cgaactcaac ggcgccaccg agtatttcat tcagaagggc ggctttctcg cggccgaaga
     2521 ttcggtcacg atggagaccg tttcccaggg ctttttcaag ggattcatga gcggcgaagg
     2581 catgttcatt cagaaggcct ccggcaaggg caagctgctg atttccagtt tcggtg
//
LOCUS       DAORHD010000674         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46380_length_2634_cov_5.369523, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000674 DAORHD010000000
VERSION     DAORHD010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46380_length_2634_cov_5.369523"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PKO06_13860"
     CDS             <1..170
                     /locus_tag="PKO06_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HNV70781.1"
                     /translation="GEPFTAHNVRVIRPGHGLHPRYLEEVLGRRATRAVERGTPLSWD
                     LIGGGERKEHS"
     gene            167..1159
                     /gene="pseB"
                     /locus_tag="PKO06_13865"
     CDS             167..1159
                     /gene="pseB"
                     /locus_tag="PKO06_13865"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016837992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HNV70782.1"
                     /translation="MTERYSLKNKVILVTGGTGSFGNAFVTYALEHLSPKKIIVYSRD
                     EFKQYEMAKRFPEKSLRFFIGNVRDQDRLYRALTGVDVVVHAAALKQVPAAEYNPFEA
                     VQTNIMGAQNVVNAAIDAGVKRVVALSTDKACNPVNLYGATKLCSDKLFIAGNAYAGG
                     KDTSFSVVRYGNVLGSRGSVIPFFKECAKTGEIPITDERMTRFWITLPQAVQLVVTAL
                     HEMQGGELFIPKIPSMKIVDLAEAIAPGCRHRVIGIRPGEKLHEMMISRDDSRHLFAS
                     DEFYIKLPDFPFWKMTTPKGSKPVSEGFEYASDTNDRWLGVAEMRRVLKNEGMI"
     gene            1196..2398
                     /gene="pseC"
                     /locus_tag="PKO06_13870"
     CDS             1196..2398
                     /gene="pseC"
                     /locus_tag="PKO06_13870"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR03588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HNV70783.1"
                     /translation="MVFIPYGKQDIDEADVRTVLETLRSDFLTQGPAIERFEKAFATE
                     VGAPLAVACANGTAALHLCSLAGGVRPGDRAVVTPISFVASANCIRYAGGTVEFADIE
                     PHSLTLSPARVAEKLEQATAAGNPIRVVVTVDLCGHPCDLVAFARLKKQYGFLWIHDA
                     CHALGASACDEQGRRRRVGEWPEIDYVAYSFHPVKHITTGEGGMVTTHDQAAAERLRQ
                     YRTHGITRQASEFVNRAEAYDENGTLNPWYYEMQALGYNFRLTDLQAALGESQLRRLP
                     GFLLRRREIANRYRESLSGLRHLNMVPVRPDVEHAYHLFVVRIDYHALGKSRARVMTE
                     LRSRDIGTQVHYIPIPMMPAYGSQLERTDLPVAWQYYREALSIPCYAGMTDTDVDRVI
                     QALKEVVT"
     gene            2437..>2634
                     /locus_tag="PKO06_13875"
     CDS             2437..>2634
                     /locus_tag="PKO06_13875"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNV70784.1"
                     /translation="MGSTRLPGKVLLKLGGRTVLEQVVRRARRASRIDRVVVATSDRA
                     EDHEIAAWCETHQVPCVRGSAE"
BASE COUNT          561 a          669 c          814 g          590 t
ORIGIN      
        1 aaggtgagcc attcacggct cacaacgtgc gggtgattcg tcctgggcat ggtttgcacc
       61 cgcgttatct cgaggaggtg cttgggcgtc gggccacacg ggcggtcgag cgcggcaccc
      121 ctctgagctg ggatctcatc ggcggcggag aaaggaagga acactcatga ccgaacgtta
      181 ctctctcaag aacaaggtta ttctggtcac cggcggtacc gggtcgttcg ggaatgcctt
      241 tgtgacctat gctctggaac acctgtcgcc gaagaaaatc atcgtgtatt cccgggacga
      301 gttcaaacag tatgagatgg ccaaacgctt tccggaaaag agtctgcgct tcttcatcgg
      361 caacgtgcgt gaccaggatc gactgtatcg cgcgcttacc ggagtcgatg tggttgtgca
      421 tgccgcagcc ctgaaacagg ttccggctgc tgagtataac ccttttgagg cggtacaaac
      481 caacatcatg ggcgcgcaga acgtggtcaa tgctgccatc gatgccggag tcaaacgtgt
      541 cgttgccttg agtaccgaca aggcctgtaa ccctgtcaac ctctacggag ccacaaaact
      601 ctgctcggac aaactcttta ttgccggcaa cgcctatgcg ggaggaaagg acaccagctt
      661 ctcggtcgtg cgctacggca atgtgctggg tagccgtggg agcgtcattc ccttcttcaa
      721 ggaatgtgcc aagaccggtg agataccgat cactgatgaa cgtatgaccc gtttctggat
      781 taccttgccg caggcggttc agctggtagt gacggcgctg catgagatgc agggggggga
      841 gttgttcatt cccaagattc cgagcatgaa gatcgtcgat cttgccgagg caatcgctcc
      901 ggggtgtcgg catcgcgtga ttggtatccg acccggagaa aaactgcatg agatgatgat
      961 ttcgcgcgat gattcgcgac atctgtttgc gagtgacgag ttttatatca aacttcccga
     1021 tttcccattc tggaagatga cgactccgaa agggtcaaaa cccgtttctg aagggtttga
     1081 gtatgcgagt gacacgaacg atcgctggct gggagtcgcg gaaatgcgtc gggtgctgaa
     1141 aaacgagggg atgatctgat gcgtacgagt ggatttttcg tggagcgttg aagccatggt
     1201 ctttattccg tacggaaagc aagacattga tgaagcggac gtgcgcacgg ttctcgaaac
     1261 gctgcgatcg gactttctga cgcaaggacc ggcgattgaa cgattcgaaa aggcatttgc
     1321 gaccgaggtt ggcgcgccgc tggcagtggc ctgtgccaat ggtactgccg cattgcacct
     1381 gtgttcactg gcagggggcg tgcggccggg agaccgagcg gtggtgactc cgatctcatt
     1441 cgttgccagt gcgaactgca ttcggtatgc gggtggaact gtcgagtttg ccgacattga
     1501 gccgcactcg ctgaccctgt cgccggctcg agtcgctgag aagctcgaac aggcgacagc
     1561 cgccgggaac ccgattcgcg ttgtcgtgac cgtcgatctg tgcggtcatc cgtgcgatct
     1621 ggtcgccttt gcccgattga aaaagcagta cggttttctc tggattcacg atgcctgtca
     1681 cgctctcggg gcgagtgctt gcgatgaaca ggggcgtcgt cgtcgtgtgg gtgagtggcc
     1741 tgaaatcgac tatgtggcct acagttttca tccggtgaag cacatcacga cgggtgaggg
     1801 gggcatggtg accacgcatg atcaggccgc ggcagagcga ttgcgtcagt atcgcacgca
     1861 tggcattact cggcaggcga gtgaattcgt caatcgcgcc gaagcatacg acgagaacgg
     1921 aacgttgaac ccgtggtatt acgagatgca ggcgctggga tacaacttcc gtctgaccga
     1981 tttacaggca gcgttgggtg agagtcagct gcgtcggtta cccgggtttc ttcttcgtcg
     2041 tcgcgagatc gcgaatcgct atcgcgaatc gctgtcaggg ctcagacatc tgaacatggt
     2101 accagtgcgc ccggatgtcg agcacgctta tcacctgttt gtcgtgcgta tcgattatca
     2161 cgccctggga aaaagccggg ctcgtgtcat gacagaactc cggtcacgag acattggtac
     2221 gcaggttcac tatattccca ttccgatgat gccagcatat ggaagtcagc ttgagagaac
     2281 agatttgccg gtagcctggc agtactatcg cgaggcactt tccataccct gttatgccgg
     2341 aatgacagac accgatgtcg accgggtgat tcaagcactg aaagaagtcg tgacatgaat
     2401 agtgaagtca tcgtccaggc ggttgttcaa gctcgaatgg gatcaacgcg acttccagga
     2461 aaggtgcttc tgaaactcgg cggtcgtacg gtgcttgaac aggtggttag acgtgcacgt
     2521 cgggcctcgc ggatcgatcg cgtcgtcgtc gccaccagtg accgagctga agatcacgag
     2581 attgcggcgt ggtgtgagac tcatcaggtt ccctgtgtgc gcggttcggc cgaa
//
LOCUS       DAORHD010000675         2633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46424_length_2633_cov_3.038402, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000675 DAORHD010000000
VERSION     DAORHD010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46424_length_2633_cov_3.038402"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..833
                     /gene="surE"
                     /locus_tag="PKO06_13880"
     CDS             72..833
                     /gene="surE"
                     /locus_tag="PKO06_13880"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV70785.1"
                     /translation="MRILVSNDDGIQARGIIALAEALVPLGEVIVVAPNAERSAASHA
                     LTLEHPLRAKPAVFPVAGVTKAYAVNGTPADCVKLALSSLLDEPPDLVATGINRGANL
                     AVDVFYSGTVAGAFEGLFRKLPSIAFSLASYEETADLSGARHFIRPIVEKLMQMKSRS
                     SVLYNVNFPALPPDQIRGIRGTRLGQVSYSENYDRRSDPAGRPYFWLKGELSIVDPSP
                     DTDVVAVRDGYVSISPFRAELTDFEALESLQRLAE"
     gene            842..1681
                     /locus_tag="PKO06_13885"
     CDS             842..1681
                     /locus_tag="PKO06_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNV70786.1"
                     /translation="MNDLSLLPDRLRLACLQFDVRRADVAGNCERVSSLMQEAAVRAC
                     HLVVLPEMWSASFAGEGLKREAECLAERQAFVAAEARRNQLWIAAGTLPEPASDDRVF
                     NTLFLFDPTGTIRWSYRKVHLFPNSSEPRWFRGADRPAQPLMLGSWVIGGGVCYDVRM
                     PELFRAQMKAQANLYVLPAQFPDPRLEHFTLLCRCRALENLAYLAGVNRIGSEGSLTH
                     SGGSLVVDPFGRVLAQRDATEGIVIADLDVAVVQAIRRQHPFLEQTKTLDQLLAGGTL
                     AHE"
     gene            1674..2495
                     /locus_tag="PKO06_13890"
     CDS             1674..2495
                     /locus_tag="PKO06_13890"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNV70787.1"
                     /translation="MSEGSELPGAWQASFTSIRELSSFRSHQCEWIAETDSTNDDLRS
                     EWEQPLPKPRLRVAEHQRRGRGQFDRCWLDVAGKSLLFSFSWALTAHDDHRLPLSCLA
                     GIALWQALGQLIPHVSPSSWYLKWPNDLWNEQGKIGGILVESSGSGSQFDAIIGIGVN
                     LSQVPILPGPVTGQLARAAAVDVSRYALLVAFCRQWDHLSRVRWSELITAYRSATSGM
                     VGRRFDFHLPDTTSVSGTVRAIDDEGALIIEDRVGARRVIRSLAQSPEEKETGRS"
BASE COUNT          495 a          747 c          798 g          593 t
ORIGIN      
        1 ccggttgcgc gcagaaacct ctcgggcgga tctgagtcgc agaatcgaat catacctgac
       61 ggaggcaaca gatgcggatc ctggtgagca acgacgacgg cattcaagcg agaggtatca
      121 ttgcgctggc agaggccctt gttcccctcg gcgaggtgat cgtcgtcgcc cccaatgccg
      181 agcgcagtgc cgccagccac gcgttgactc tcgaacatcc gctgcgggcg aagccggcag
      241 tgttccccgt tgccggggtt acgaaagcat acgccgtgaa cggcactccg gctgattgcg
      301 tgaaactggc cctttcctcg ctgcttgacg aaccgcctga cctggtggca accggcatca
      361 atcgcggagc caatctggcg gtcgacgtct tctattccgg aaccgtggcc ggtgcgtttg
      421 aaggcctgtt ccgaaaactg ccttcgattg ccttctctct cgccagctat gaagagaccg
      481 ccgatctgtc gggggcgcgt cacttcattc gacccatcgt tgagaagctt atgcagatga
      541 aaagcagatc gagtgtcttg tacaatgtga attttcccgc actcccgcct gaccagatca
      601 ggggcattcg tggcacccga ctggggcagg tgagctattc cgaaaactat gatcgtcgtt
      661 ccgacccggc cggtcgtccg tatttctggc tcaagggcga actctccatc gtcgatccca
      721 gccctgatac agatgtcgtg gcggtgcgcg acggttatgt ttccatatcg ccgttccgcg
      781 ccgaattgac ggactttgaa gcgctcgaat cacttcagcg actggccgag taggaattgg
      841 cgtgaacgac ctgtcactgc tgcctgatcg cctgcgcctg gcctgtctgc aattcgatgt
      901 gcggcgtgcc gatgtcgccg gaaactgcga gcgggtctct tcgttgatgc aggaagcggc
      961 tgtcagggct tgccatctgg tcgtcctgcc tgagatgtgg agcgcgagtt ttgccggcga
     1021 agggttgaaa cgtgaggcgg aatgcctcgc ggagcggcag gcgtttgttg ccgccgaagc
     1081 gcgtcgaaat caactctgga tcgcggctgg aacgttaccg gaacctgcca gtgatgatcg
     1141 agtgttcaac acgttgtttc tgtttgatcc gactgggacg attcgctgga gttatcgaaa
     1201 ggtccatctg ttccccaact cctccgaacc gcgatggttt cgtggagctg accgacccgc
     1261 acaaccgctg atgctgggtt cgtgggtgat cgggggcgga gtctgttatg acgtgcgcat
     1321 gcccgaactg tttcgtgcgc agatgaaagc gcaggcgaat ctgtatgtgc tgccggcgca
     1381 gtttcctgac ccgcgtcttg agcactttac cctgctctgc cggtgccggg cccttgagaa
     1441 tctggcctat cttgccggtg tcaaccgcat cggaagcgaa ggttctctga cgcattccgg
     1501 tggcagtctc gtggtcgatc cctttggacg ggtgctcgcg cagcgagacg ccacggaggg
     1561 aattgtcatc gctgatctcg atgttgccgt ggtccaggca attcgtcggc aacacccgtt
     1621 cctcgagcag accaagactc ttgatcaact gttggccggg ggaaccctgg cacatgagtg
     1681 aagggtccga gttgcccggg gcctggcagg cctctttcac gtcgatccgg gaactgtcgt
     1741 catttcgcag ccatcagtgt gaatggattg ctgagacaga ttcgaccaat gacgatctgc
     1801 ggagcgaatg ggaacaaccg cttccgaaac cgcgtctgcg cgtggctgag catcaacgtc
     1861 gcggcagagg gcagttcgat cgctgctggc tggatgtggc gggaaaatcg ctgctcttct
     1921 cattttcgtg ggcgttgacc gctcacgatg accatcgctt gccgctttcc tgtctggccg
     1981 gaatcgctct gtggcaagca cttggacaac tgatccctca cgtcagtccg agttcatggt
     2041 atctgaaatg gccgaacgat ctctggaacg agcagggaaa aatcggcggt atactcgtcg
     2101 aatcctccgg cagcgggtcc cagttcgatg cgatcatcgg tatcggcgtg aatctctctc
     2161 aagtgccgat tctgcctgga ccagtgacag gccaactcgc acgcgccgcc gcggtcgatg
     2221 tctcacgcta cgccctgctt gtggcgttct gtcggcaatg ggaccacttg agccgggtgc
     2281 gatggtccga gttgataacg gcataccgct cggccacgtc cggaatggtg ggtcgtcgtt
     2341 ttgattttca tctgcctgat acgacgtctg taagcggaac ggtgcgcgcg atcgatgacg
     2401 aaggtgcctt gattatcgaa gacagagtgg gtgcccgtcg tgtgattcgc tctctggctc
     2461 aatcaccaga agaaaaagaa accgggcgat cctgatggac cgcccggtaa cgctcgtgaa
     2521 ttctattcta ccggaatcga cagcttgtac gagaacggac ctttctcgga ttcgccgtat
     2581 acccacacat aggcttccgc ggtgggctgc gagaaagtca tgtcgatcat gtc
//
LOCUS       DAORHD010000676         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46443_length_2632_cov_5.292976, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000676 DAORHD010000000
VERSION     DAORHD010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46443_length_2632_cov_5.292976"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKO06_13895"
     CDS             <1..882
                     /locus_tag="PKO06_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70788.1"
                     /translation="ASVRAQTRSLLAAQEQELKPWGSIHELAIPTGRLQKPFALQVHQ
                     LPQNRTWGLYAQTSDGDWIWQSSINASGTAAAVAQAGVFGVFCDTMAPRVVMTTTFPQ
                     TPLSDARPVIEGRVEEFGSGLNGAVEVVIDGQAQTVPVDTASGAIRYQPLEPLSSGEH
                     EVRIRAVDRGGNVGETAAIRFAVHAPLQVAEMITFPNPVRERVTLRITANRNDLDLDA
                     MTIKIYDVSGDRVAELSGSAPRLEAGIYRYDVVWDLRNDDGARVANGLYLARMEIRDP
                     ENPGKKITKTQKIAVLK"
     gene            complement(966..1340)
                     /locus_tag="PKO06_13900"
     CDS             complement(966..1340)
                     /locus_tag="PKO06_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70789.1"
                     /translation="MSKILIIEDDDQIRKMLRQMLERGGYEVFDAPNGKDGNRAFRKN
                     PVDLIITDIFMPEMDGLETIIELRRDFPQLKIIAISGGARGGSFDFLPIAESFGAAKI
                     LKKPFTREEILNAVKEVLEAKA"
     gene            complement(1353..2180)
                     /locus_tag="PKO06_13905"
     CDS             complement(1353..2180)
                     /locus_tag="PKO06_13905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70790.1"
                     /translation="MDQRRKARVSTRSSPRVRRSSVRLKACERRLERERRVLQARKLS
                     AIAALAGGFAHDFNNMLAAINGFTELALDDIPADSPARENLTEVFAGVKKARHLVDQI
                     HAIGSRNRRGFKTVSLSRLLEDRLSHLRTRFPSTVQLQTNVCSAVPTQNPDFPITANP
                     DEIGLVIEHLVQNGFEALPASGGRITIELRYPGSGQIELSVKDTGSGIAPQVMEHLFE
                     PFFTTRQKARGLGLAMCHGIVDKHRGSISVQSQLGHGTCVKICLPQEDAANNGSATK"
     gene            complement(2214..>2632)
                     /locus_tag="PKO06_13910"
     CDS             complement(2214..>2632)
                     /locus_tag="PKO06_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV70791.1"
                     /translation="FPEDKQRRLAELRLQHGPILMLGDGINDAPALAAADLGVAMGRT
                     GTDAVLETAGAVLLHDRLDRLPLLLAISQRCQSLIKLNMGLAIGLKLLVFGLSLFGLA
                     TLWMAVIADTGASVLVVVNGLRALKPPSGFADLSTS"
BASE COUNT          544 a          728 c          798 g          562 t
ORIGIN      
        1 gcatcggtgc gggcgcagac acgcagcctg ttggcggctc aggaacagga actcaagccg
       61 tggggctcga tccacgaact ggcgattccc accggccgcc tgcagaaacc gtttgctctg
      121 caggtgcatc aactgccgca gaatcgaacc tggggcctgt acgcgcagac ctcagacgga
      181 gattggatct ggcagtcttc gatcaatgcc tcgggaacag ccgcggccgt ggcacaagcg
      241 ggcgtattcg gcgtgttctg cgatacgatg gctcctcggg ttgtgatgac gacgaccttc
      301 ccgcaaaccc cgttgagcga tgcacgtccg gtcattgagg gacgggtcga ggagttcggt
      361 tccggactga atggcgcggt cgaagtggtg atcgacggtc aggcacagac ggttcctgtc
      421 gatacggcga gtggtgcgat tcgctaccag cctctcgaac cgctctcttc cggcgagcac
      481 gaagtgagaa ttcgcgcggt cgatcgtggt ggcaatgtcg gagagacggc ggcgatacgg
      541 tttgcggtgc atgccccctt gcaggtggcc gagatgatca ctttccccaa cccggtccgt
      601 gagcgagtga ccctgcgcat taccgcgaac cgcaacgatc ttgatctcga tgccatgacc
      661 atcaagatct atgatgtgtc gggtgatcgt gttgccgagt taagcggatc agctccgcgg
      721 ctcgaagcgg gaatctaccg ctatgacgtc gtctgggatc tccgaaacga tgacggggcg
      781 agggttgcca atggcctgta tcttgcccgc atggagattc gcgatccgga aaacccgggg
      841 aaaaagatca ccaagacgca gaagatcgcc gttctgaagt aacgagtact cgcgaaaaaa
      901 ccgggtgaga caatcgatgt ctcacccggt ttgctctttc gtgaatagag ctctgggagc
      961 gaagatcagg ctttggcttc caggacttcc ttgacggcgt tgagaatctc ttcacgtgtg
     1021 aacggcttct tcaggatctt cgcggcacca aatgactcgg cgatgggaag aaaatcgaag
     1081 ctgccgcccc gtgctccccc ggaaatggcg atgatcttga gttgcggaaa gtcgcgtcgg
     1141 agctcgataa tggtctcgag tccgtccatt tccggcataa agatgtcggt aatgatcagg
     1201 tctacggggt ttttccgaaa tgcccgattt ccatccttgc cgttcggggc gtcgaagacc
     1261 tcgtaaccgc cgcgttcgag catctgacga agcatttttc gaatctgatc gtcgtcttcg
     1321 atgatgagaa tcttgctcat atcgctcccg tcctactttg ttgcgctgcc attgtttgct
     1381 gcatcttcct ggggcaggca gatcttgaca cacgttccgt ggcccaactg gctttgcacc
     1441 gaaatcgacc cacgatgctt gtccactata ccatgacaca tcgctaaacc caagcctcgt
     1501 gctttctgtc gagtggtgaa gaagggttca aacagatgtt ccatgacctg gggggcaatg
     1561 ccggaaccgg tatctttgac agaaagttcg atctgcccgg aacccggata tcgtaactcg
     1621 atggtgattc gtcctccact tgccggcaag gcttcgaacc cgttctgaac cagatgttcg
     1681 atgaccagcc cgatctcgtc tgggttggcg gtgatgggaa aatcaggatt ctgcgtggga
     1741 acggcactgc agacattggt ttgaagctga accgtcgatg gaaatcgggt gcgcagatgg
     1801 ctcagtcggt cttcgagcag acggctgagg gaaacggtct tgaagccacg ccggttgcgg
     1861 ctgccgatag cgtgaatctg atcaaccagg tgccgggcct tcttgacccc ggcgaacact
     1921 tcggtcagat tctcacgggc aggagagtcg gcgggaatat cgtcgagcgc cagttccgta
     1981 aagccgttga tggcggccag catgttgttg aaatcgtgcg cgaatccccc ggccagtgcg
     2041 gcgatcgcgc tgagtttgcg tgcctgcagt actcgtcgct ctcgttcgag gcgtcgctcg
     2101 caggctttga gacggacaga cgaacgccga acccgggggg aggatcgggt tgagactcgg
     2161 gctttgcggc gctgatccat gctcactctc cgaatgtgga tgctttcagg gtatcaggat
     2221 gtcgagagat cggcaaatcc cgatggtggt ttcaaagctc gcagaccgtt gacgaccacc
     2281 agtacggatg ccccggtatc cgcgatgacc gccatccaga gtgtggcgag cccgaacagc
     2341 gaaagcccga acaccagaag tttcagtcca atcgcgagac ccatgttcag tttgatcaga
     2401 gattgacaac gctgactgat ggccagcaat aatggcagac gatcaaggcg gtcgtgcagc
     2461 agaaccgctc cggcggtttc cagcacggcg tcggttccgg ttcgacccat ggccactccc
     2521 agatctgctg ccgccagcgc gggggcgtcg ttgattccat cgcccagcat cagaatgggc
     2581 ccgtgctgaa gacgtagttc agccagccga cgctgcttgt cttcagggaa ac
//
LOCUS       DAORHD010000677         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46861_length_2617_cov_2.359485, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000677 DAORHD010000000
VERSION     DAORHD010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46861_length_2617_cov_2.359485"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..310)
                     /locus_tag="PKO06_13915"
     CDS             complement(134..310)
                     /locus_tag="PKO06_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70792.1"
                     /translation="MGKKLFFLSLVFLASVMARDWYANRPKEPKRPKNAPAPVAVTIA
                     RKGANCNKAHENVR"
     gene            complement(314..922)
                     /gene="pilO"
                     /locus_tag="PKO06_13920"
     CDS             complement(314..922)
                     /gene="pilO"
                     /locus_tag="PKO06_13920"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNV70793.1"
                     /translation="MADFSLMEWLEKAKADPKVAAQPLIAIVAVAALGWKFGYAPVQT
                     EIAKELKKNKGVQDQIKGLETAVANIEDIKIGVAEMRKKWDEVQALCYNKNEAHKFLQ
                     DLRQIGRDVGLDIKNITPQPTIQKTFDTLIYEEYPIKIAFSGSLAQLGIFLRKLEKHP
                     RITFLELPPIIPDASGVLRLDLLPSTILLSDQRVEPPPPPPE"
     gene            complement(956..>2617)
                     /gene="pilM"
                     /locus_tag="PKO06_13925"
     CDS             complement(956..>2617)
                     /gene="pilM"
                     /locus_tag="PKO06_13925"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNV70794.1"
                     /translation="RKMLTKHLTELLYDNQIQTKRAISLVSGRAVTIRYIEIPVPEKP
                     EMLADAVQVEAAKQMPFSMENAALGYTAFPQVKREDKQLIPIMVAALQKDIVQVITDN
                     LKGGGLSNDSLLTLPQSLELLIGESLKPPAGKDLRAAVIHCGHKTTSIMIYKNGILNF
                     YRDINMAGETITEAILAGGEVEGEKISFANIDEVDELKHKIGVLPPDDVKALKEKKEK
                     FVAQQIFTTVEKIFQHIQLSVSFYISQFGESALDRIYLSGGTSAMKNFKEFIGESLEV
                     SVEIIDPMKYVPLGEVNFPKEKMENEAPAIAPALGMALYNYQPDIVNFIDILYPHRRS
                     QSSTDFSKAGAKFTQGLSNKLGISFELDETKLRIIAVLLAIIIIAGGAYPLVKVRQNL
                     AKTKSDFKKFNTQLEELTASNKEVTDLNAEREQLEKLAGFADELKKYSFPTSEALLEL
                     ATIVPKQIFLISGEIRNESGRRIFKITGHSDNSDRVFEFLKVFATSTRFLKNPSLEAT
                     EEVPIDESRYFIRFVLNGQIVIPEPEKPAAPEGEGDSSEPAPSGE"
BASE COUNT          572 a          636 c          699 g          710 t
ORIGIN      
        1 tgatgccaaa aagttgcaga cttttcggaa tggatcatgc ggctgtgcga tgcgcggcga
       61 aagttttttg ggacgcgcgc ttatggctga aggcgcagcg gggatcaagg gccgtgcgcg
      121 ttcggcgagt cggctatcgc acgttttcgt gcgccttatt gcaattcgca ccttttcgtg
      181 cgattgtgac ggccacgggg gcgggagcat tcttggggcg tttgggttct ttcgggcggt
      241 tggcatacca atcgcgtgcc atgaccgacg ccagaaaaac gagactcaga aagaagagct
      301 tcttgcccat gagttattcc ggtggcggag gtggcggttc aacgcgctga tccgacaaga
      361 gaatcgtgct cggaagcaag tcaagcctga gaactccgct ggcgtccggg atgatcggcg
      421 gcagttcgag aaaggtgatt ctgggatgtt tctcgagctt gcgaagaaag atcccgagtt
      481 gggcaagaga ccccgagaac gcgatcttga tcgggtattc ttcgtaaatc aacgtgtcaa
      541 aggttttctg aattgttggt tgtggtgtga tgttcttgat atccagaccg acatctcgac
      601 caatctgtcg caagtcctgc agaaacttgt gcgcttcatt cttgttatag caaagcgctt
      661 gcacttcatc ccacttcttg cgcatttccg cgacaccgat cttgatgtct tcaatgttgg
      721 cgacagcggt ttcgagaccc ttgatctgat cttgaacccc tttgtttttc ttcagttcct
      781 tggcgatctc ggtttgcacg ggtgcatagc caaacttcca gccgagcgcg gcaactgcca
      841 caatggcgat cagaggctgg gcagccacct ttgggtctgc cttggccttt tcgagccatt
      901 ccattaggct gaaatcagcc acaagtaggt cctttcgtaa tcatcaactt gtactctatt
      961 ctcccgaagg cgcaggttct gaagaatctc cctctccctc aggtgcagca ggtttctccg
     1021 gctcaggaat gacaatctga ccattcaaca cgaagcgtat gaaatacctg gattcatcga
     1081 ttggcacctc ttcggttgct tcaagtgacg ggttcttcag aaagcgggtc gaggtcgcaa
     1141 atactttgag aaactcgaag acgcggtcgg agttatcgga gtgccccgta atcttgaaga
     1201 ttctcctgcc gctctcgttg cgtatttcac ccgagatcag aaaaatctgc ttgggaacaa
     1261 tggttgccag ctcaagaagc gcttctgaag ttggaaaaga gtactttttc aactcgtcgg
     1321 caaacccggc tagtttttcg agctgttccc gctctgcatt caggtcggtg acctccttgt
     1381 tcgaagcggt gagttcttcc aactgtgtgt tgaacttctt gaagtcactt ttcgtttttg
     1441 ccaggttctg gcgaacttta accagcggat acgctccgcc tgcgattatg atgatcgcga
     1501 gcagaacagc gattattcgt agtttggttt cgtcaagttc aaaagaaatt cccagcttgt
     1561 tcgataaccc ttgggtgaac ttcgctcccg ccttggaaaa gtccgtcgaa gactgtgacc
     1621 ggcggtgcgg atacagaatg tcgataaagt tcacgatatc gggttgataa ttgtagagag
     1681 ccatccccag cgcgggcgcg attgccggag cttcattttc cattttttcc ttggggaagt
     1741 tgacttcgcc aagtggaaca tacttcatcg gatcaatgat ctcgaccgat acttccagac
     1801 tttcgccaat gaattccttg aaattcttca tggccgaagt gccgcctgaa agatagattc
     1861 ggtcaagcgc actctcgcca aactgcgaga tgtagaagct cacagagagc tgaatatgtt
     1921 gaaaaatctt ctcaaccgtc gtgaatatct gctgagcgac gaatttttct tttttctctt
     1981 tgagagcctt gacgtcgtcc ggtggcagca cgccgatttt gtgttttaat tcgtcaactt
     2041 cgtcaatgtt ggcaaaggag attttctccc cttcaacctc acctcccgca agaatggctt
     2101 ctgtaatggt ttcaccagcc atgttgatat cgcggtagaa gttcagaatg ccgttcttgt
     2161 agatcatgat cgatgttgtt ttgtgaccgc agtggatgac tgctgcgcgc aaatccttgc
     2221 cggccggagg cttgaggctt tcgccgatca gcagttcgag agattgaggg agggtcagga
     2281 ggctatcgtt ggataagccc ccacctttga gattgtccgt gataacctgg acaatatcct
     2341 tctgtaacgc agctaccatg atggggatca gctgcttgtc ttcgcgcttt acctgcggaa
     2401 atgcggtgta tccgagagcg gcgttttcca tggaaaacgg catttgcttg gcggcttcaa
     2461 cctgaacagc atcagcaagc atttcaggct tctcgggaac cggaatttcg atgtagcgaa
     2521 tggtgacggc ccgccccgaa acaagagaaa tggcgcgctt ggtttggatc tgattgtcat
     2581 agaggagttc ggtaagatgc ttcgtgagca tcttgcg
//
LOCUS       DAORHD010000678         2617 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46862_length_2617_cov_2.286495, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000678 DAORHD010000000
VERSION     DAORHD010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46862_length_2617_cov_2.286495"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..770)
                     /locus_tag="PKO06_13930"
     CDS             complement(159..770)
                     /locus_tag="PKO06_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70795.1"
                     /translation="MGPQVKIDRESVRIGDSSSLRVVHPAFSEVYADLRFSPDGTNHT
                     TVSWQGNPEISTQKAALIAGVPMLGIGMIPFLLIFVKTGAVLGLLPMFFGLLFAAVVI
                     FYSLKTRARGMEKSLTTYFENVQMLGDLEEQKATRAELESLRKKEANRDTRSETEAKP
                     SGVSDLPSNVESSTQAKIPVQHSPLAEPDESENAPPPRPRERE"
     gene            complement(1442..1852)
                     /locus_tag="PKO06_13935"
     CDS             complement(1442..1852)
                     /locus_tag="PKO06_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HNV70796.1"
                     /translation="MTFERSTESDSLPTSFTALSIVYPHGTSIARGTKTIEVRSWLPP
                     QVPIRNLLIVENRVFLSETLQEDPDGRAVAIVDVIEVHPWRQDEVKSACSRGWQPGYF
                     SWVLENVRPLPGMDIIPARRKLYSVLFPQGLHHL"
     gene            2034..2474
                     /locus_tag="PKO06_13940"
     CDS             2034..2474
                     /locus_tag="PKO06_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013917522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV70797.1"
                     /translation="MQPLIDHIQITVQNMERAIPFYDRLMPLLGFDPKKRSSAHIAAH
                     EFHVVEYSHPLLAFAITSPRPGLEKESVHRRRPGSLHHLAFRAESRAEVDRLHLELVK
                     IGATIVTPPREYPEYTPPGYYAVFFKDPEGIKYEIVHAPHPTHA"
BASE COUNT          593 a          751 c          698 g          575 t
ORIGIN      
        1 aacgaagtcc tgatcatcac cctcgaagac gagaccggat atgtccaggc tcatttctct
       61 cgatatcgtc tcggaatctg cttgacgccg agctatgatt caggcgattt cagtatcgaa
      121 atgtgaaaac tcgagcatct tcgctgaaac atggccgttc actcacgctc acggggtcgg
      181 ggtgggggag cattttccga ctcgtcgggt tctgcgagag gcgagtgctg aaccgggatc
      241 ttcgcctgcg tagacgactc cacgttggac ggtaaatccg acactcctga aggcttcgct
      301 tcggtttccg agcgagtgtc gcggttcgct tctttctttc tcaagctctc gagttcggcg
      361 cgggtcgctt tctgttcttc aagatcgccg agcatctgca cgttctcgaa gtaggtggtc
      421 agcgattttt ccataccgcg agcccgggtt ttcagcgagt agaagatgac tacagctgca
      481 aacagcagac cgaaaaacat cgggagcagg cccaggacag cgccggtttt tacgaagatg
      541 agcagaaagg gaatcattcc gatacccagc atcggaactc cggctatcaa tgcggctttc
      601 tgtgtcgaga tctccgggtt gccctgccag gacacggtcg tgtgattcgt accatcgggc
      661 gaaaaccgaa gatcggcgta gacctcgctg aaggcagggt gaaccacgcg caagctgctg
      721 gaatctccaa tgcgcacgct ctcacgatcg attttgacct gtggtcccaa ctgatgtttc
      781 ccccctgcca gaccgtggtt tcattcatca gaaagtgtct gagaaaggct tcctgaacct
      841 gttctggcga gccgcgcacc cgaaatcgcc gatgccgatt agcgacatcg cgtgccgctt
      901 tctgcaagat ctcgggtttg atacccagtc gctcactcgc ggcctgtaga tcatcggtcg
      961 aaaactcgtt ttccgagagg ttggtctgca ggctctgcag ttccagcgat cgtttcagaa
     1021 tggcttcgaa ttcttcccgt ttcattcgtg tcttctcgcg tcgtatcgta aaatgtgcgt
     1081 tatcgaaacg atatgttatc agattctccg cattacatcc tttgtctatt cctgagacga
     1141 cgcacaatgt gatacagcca gacgacgaaa aaccagattg ttgcaagtgc tgtcagaaag
     1201 ccggaactca aagcgatgat ccggcacagg gatctcagca gatgatccgc gtcagacaga
     1261 gtcgccgcat acagatacag gttcgtcgtc acaagccagg ggacagctgt gatgaggaga
     1321 aacagtggaa aagtcagaat ttcacgtgcc agaaggggac ctcgtggaac agcctcccga
     1381 gagatccggt ttgcaggctc gcccgtcaca gcaacgcctt cctgcctcgg ttctgagaca
     1441 ttcacagatg atgcagcccc tgcggaaaca gtaccgagta aagtttgcgg cgcgcaggga
     1501 tgatgtccat tcccggtaat ggcctgacgt tttcgagcac ccaggaaaaa taacccggtt
     1561 gccatcctct ggaacaggcc gacttcactt catcctgtcg ccacgggtgg acctcgatga
     1621 catcgacgat ggcgaccgcc cgaccgtccg ggtcttcctg cagggtttcc gagagaaaaa
     1681 cccggttttc cacgatcaac agattgcgga tcggaacctg cgggggaagc cacgatctga
     1741 cttcgatcgt tttcgttcct ctggcaatgc tcgttccgtg cggatacaca atggatagtg
     1801 cggtaaaaga ggtcggcaga gaatcgcttt ccgtggaacg ctcaaacgtc attcaggaac
     1861 acccccgtcg catcatacca tgcaacaagg ctcgattcgt cggtttcgcg gtggtgtcgt
     1921 ggaacaatgc tttcggacgt gatagtctga catggccgat tggccagcga ttgagccagc
     1981 aggcccgccc cgctgatcag gacagaaagc cccccgaaag gaagtagttc cccatgcaac
     2041 cgttgataga tcacattcag atcacggtcc agaacatgga acgggcgatt ccgttctatg
     2101 atcgactgat gccgctgctg ggattcgatc cgaagaaacg ctcgagtgct cacatcgctg
     2161 cgcacgagtt tcacgtggtc gagtacagcc accctctgct ggcattcgcc ataacctcac
     2221 cccgccccgg cctcgagaag gaatccgttc atcgccgcag acccggttct ctgcaccatc
     2281 tcgccttcag agccgaatca cgcgccgaag tggatcgtct gcacctcgaa ctcgtcaaga
     2341 tcggcgcgac gatcgtcact ccgccgcgtg agtatcccga gtacacgcca cccggctact
     2401 acgccgtgtt cttcaaagac cccgagggca tcaagtacga aatcgtccac gcgcctcacc
     2461 ccactcacgc gtagggcgcg tatccggggc agagaatgcc gagacatcgc ctcgaaagga
     2521 tattcacacg atggctgacc ggctgaccat cgagaatcga acattttgcg accaggacat
     2581 tcatcaacca tcctttgggt gtggtgacta tgaaatc
//
LOCUS       DAORHD010000679         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46878_length_2616_cov_3.640375, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000679 DAORHD010000000
VERSION     DAORHD010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46878_length_2616_cov_3.640375"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKO06_13945"
     CDS             <1..794
                     /locus_tag="PKO06_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70798.1"
                     /translation="AFTERAKAFGCTPPRGILLLGVQGCGKSLVAKAVSSLWSVPLLR
                     LDMGRIFSSLVGSSEENVRRAIRTAESVAPAILWVDEIEKAFSGMQSSGQSDAGVSSR
                     VFGTFITWLQEKTSPVFVIATANRIRDLPPELFRKGRFDDIFFVDLPSAIEREQIFTI
                     HLKKKGRDPAKFDLASLSRVSDGYSGSEIEEAVSSALYEAFFQNRELETEMILQTLKE
                     TFPLSRTMREDIEELRRWAGERARPASWAPSEAGSGRLQRALEIT"
     gene            808..1272
                     /locus_tag="PKO06_13950"
     CDS             808..1272
                     /locus_tag="PKO06_13950"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HNV70799.1"
                     /translation="MSAFVVLVPVMASWPVFTAAAAAAGAALGLQIAKGLASSHAAQQ
                     QQEENAVDVAVPQSHALAGSIDEGESLTLRGNGFTVFFRKTGRGDCEMRVEGAAKSKE
                     ELETLGKQLLNKIAQQYAYQRVTAEMKKRGFQVVQEKVDEEQNIRLTVRRWK"
     gene            1282..2094
                     /locus_tag="PKO06_13955"
     CDS             1282..2094
                     /locus_tag="PKO06_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70800.1"
                     /translation="MGRFQWLEFDDPATKSGTPAPAGPELESGEDFTNPERVLRLADE
                     AYRRLDYERALRLFSKVLTLDPNLEPGWAGQLKCLLDLNEIPEALTWAIKAQKLFPKS
                     AEIIASRACALARQGNLSEAIAFSDGAMKAGLIGWYPWVVRGEILAASNSANAEFCMM
                     KARELAPKEWLVALKTAQAYARTPQEEKAVEFYKVVLSERADLAEAWYELGQLQVRIG
                     LTSAAIVSLQTCVKLVPDRKKYSDALGQALRVNPLETLWRWVKRLLKGVWRS"
     gene            2091..2291
                     /locus_tag="PKO06_13960"
     CDS             2091..2291
                     /locus_tag="PKO06_13960"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HNV70801.1"
                     /translation="MTKKEEIEVTIKADGQVDLHLVGFGKACDDYIKLFQEILNAQIK
                     DKKFTSEYYSDVKTDQHQRTKS"
BASE COUNT          571 a          686 c          856 g          503 t
ORIGIN      
        1 aggcttttac cgagcgggcg aaagccttcg ggtgcacgcc gccgcgggga attctgctgc
       61 tcggggtgca gggctgtggc aagagtctgg tcgccaaagc cgtcagtagc ctctggagcg
      121 tccccctgct gcgtctcgac atgggtcgca tcttttcgag tctggtcggt tccagcgaag
      181 aaaacgtgcg acgagcgatt cgcacggccg aatcggtcgc gccagcaatt ctctgggtcg
      241 atgaaattga aaaggcgttc tcggggatgc agagctcggg gcagagcgac gccggcgtgt
      301 cgagccgcgt gttcggcacc ttcatcacct ggctccagga aaaaacgtca ccggtgttcg
      361 tcatcgcgac cgccaaccgc attcgggatc tgccacccga actgtttcgc aagggtcgct
      421 ttgatgacat cttcttcgtc gatctgccgt cggcgatcga gcgcgaacag atcttcacca
      481 ttcacctgaa aaagaaagga cgtgacccgg caaagtttga tctggccagt ctgtcgcgtg
      541 tcagtgacgg gtactctggc tcggaaatcg aagaagcggt ttcttcggcg ctgtatgaag
      601 cattctttca gaatcgtgaa ctcgagacgg agatgattct gcagaccttg aaggagacgt
      661 tcccgctgtc gcgcaccatg cgcgaggata ttgaagaact gcgccgttgg gcgggtgagc
      721 gggcacgacc cgcgtcgtgg gctcccagcg aagctggatc cggccgcctg cagcgggcct
      781 tggaaatcac gtaaggagga acgacacatg tcggcatttg tggttctggt gccggtgatg
      841 gcgagctggc ccgtgttcac cgccgcggcc gcggccgcag gagcggcctt gggcctgcag
      901 atcgccaagg ggctggcgtc ttcacacgcc gcgcaacagc aacaggaaga gaatgcggtc
      961 gatgttgccg tgccgcagtc acacgctctg gccggatcga tcgatgaggg tgagagtctg
     1021 accttgagag ggaacgggtt taccgtgttc tttcgcaaga cggggcgagg cgactgcgag
     1081 atgcgggtcg aaggagccgc gaaatccaaa gaagaactcg aaaccctggg aaaacagttg
     1141 ttgaacaaga ttgcacagca gtatgcctat cagcgcgtga ccgccgagat gaagaaacga
     1201 gggtttcagg tcgtgcagga aaaggtggat gaagagcaga acattcgcct gactgttcga
     1261 cgctggaagt aaggagcacg cgtgggtcgg tttcagtggc ttgagttcga cgatccggca
     1321 acgaaatcgg gaacccctgc gcctgcaggt ccggagctgg agagcggcga agatttcacc
     1381 aacccggagc gggtgttgcg actggccgat gaagcctacc gtcgacttga ttacgaaaga
     1441 gcgctgcgcc tgttctccaa ggtgcttacg ctcgatccca atctcgaacc gggctgggcg
     1501 ggacagctga agtgcctgct tgatctgaac gagatccccg aagcgttgac ctgggcgatc
     1561 aaggcacaga agctgtttcc gaaaagcgcg gaaatcattg cctcgcgggc ttgtgcactg
     1621 gcacgacagg gaaatctgag cgaagcgatc gcgttctctg atggcgcgat gaaagcagga
     1681 ttgattggct ggtacccctg ggtcgtgcgc ggcgagattc tggccgcgtc gaactcggcc
     1741 aacgcggagt tctgcatgat gaaggcgcgt gaactggccc cgaaggaatg gctggtagct
     1801 ctgaagacgg cccaggccta cgcacgcaca ccgcaggagg aaaaagcggt cgagttctat
     1861 aaagtcgtct tgtcagaacg agctgatctg gctgaggcat ggtatgaact cggacagtta
     1921 caggtgcgca ttggcctgac gagcgcggcc atcgtgtcgc tgcagacctg cgtgaaactg
     1981 gttcctgatc gtaaaaagta tagtgacgcc ctgggacagg cgctgcgggt taacccgctg
     2041 gaaaccctgt ggcgatgggt gaaacgactg ttgaaaggag tctggcgctc atgaccaaga
     2101 aagaagagat tgaagtgacc atcaaagcgg acggacaggt cgacctgcat ctcgtcggct
     2161 tcggcaaggc ctgtgatgac tacatcaaac tgtttcagga aatcctcaat gcccagatca
     2221 aagacaagaa gttcacgtcg gaatactaca gcgacgttaa aaccgatcag caccagcgca
     2281 ccaaaagttg agtgtcagat catcatcgag gtcgatggaa ccgtggtttt cacggacctg
     2341 tatgaagatc tggctgaggt ggctgaagtg ttgaagcggg gagagtccga gttgtcgtca
     2401 gcgcctgcgg gcgactgaca ttcaaaggag aggtggccta ttcgctgtcg agagtgcggt
     2461 gaactggctc ccgcggggag cacgcagtgt ccggcctgtg gtcgtgattc gctggtgccg
     2521 ctctcgggtg gcgacatcct gccgccgcgt gctacgcagc gaacccgcgt cgatgatctg
     2581 acggcacgcg gcggcaggat gtcgccaccc gagagc
//
LOCUS       DAORHD010000680         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46881_length_2616_cov_3.249121, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000680 DAORHD010000000
VERSION     DAORHD010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46881_length_2616_cov_3.249121"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKO06_13965"
     CDS             complement(<1..243)
                     /locus_tag="PKO06_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70802.1"
                     /translation="MMRRLLLIAWLLLVCGTMSHAQETPVNPPAKPPADDLITPVDSK
                     PITLDEALQRRLKVLARPARSAILLIADGLGASALRL"
     gene            complement(306..2468)
                     /locus_tag="PKO06_13970"
     CDS             complement(306..2468)
                     /locus_tag="PKO06_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70803.1"
                     /translation="MILILIDALLLILAHVGTALIQSDAIRWDMRLSDLAGVVGMSSD
                     LWIGVVLVFLALTVVRAVGNEQEKKQWRTLLAALSQRLGWRLWLLAALLMLPQRLMDT
                     NSGYTCIQPESVLSLLLLGLDWSRTFAALIWLRRCAAPPEEELAARQPGPSDIATPRR
                     SSIFWLGLLILTCGLIGQVALLKGIPRVQDEIIQEWQSRLFRLGALHTPPLPVRESFL
                     PDPSIDENGTWIYSTYQPAYALLKALFQIVNLDVLLNPLLGVLCLLLLSRVCRQVPRF
                     DVNRYAVVLFACSPFLLLMSASRMNHLLGLCCLLLAANGLAEFVAKRRWRGILLASVA
                     VSLLLLTRRVEGAALALSLCVSLFLIAEGWAVRLGGSALITGAGVVAALVQSRWAVAI
                     AGDPLLAVRHVQASATILQQLTPGLLWSNNLDNLLGFAGWAFGGGLAGWAGFALLRER
                     VTLESQVCRTIETFFCLHATALILGYSWYYFQDFCYGPRYLFPLLPAAAWGAARLLGQ
                     LDTIHPRAGSRVLRLTIGVGILVMTQSWWAILAPDFWHIRADLASVIAEQVPTNSLVF
                     LRHPARIRLDVLRQLRALRVSPELLQRSVVSDAVDYDAFLEALKRLPTGATEPRILEL
                     LLDFEHQARGHRPEGFAINGMEVIRLNHPFLGTGSRILALDLGDEPNMRLQAAYSSYQ
                     PVLLYRRGQRYGIASMTSCGLARFDEPRRVPEVEAHER"
BASE COUNT          653 a          806 c          733 g          424 t
ORIGIN      
        1 aagccgcagc gcggaagcac ccagaccgtc agcgataagc agaatcgcac tgcgtgccgg
       61 tcgcgccaga accttcaatc gacgctgcag ggcttcgtca agcgtgatcg gcttggaatc
      121 aacgggagtg atcaggtcat cggccggcgg tttcgcgggt ggattcaccg gggtttcctg
      181 cgcatgggac atcgtcccgc agacgagcaa caaccacgcg atcagcaaca gacgtcgcat
      241 cattccatcc tcctcaagca atttgtgtct attctagcac accccatcac catcaccgcg
      301 ttccgctacc gttcgtgggc ttctacttca ggaactcgac gtggttcatc aaaacgcgcc
      361 aaaccgcagg aagtcatcga ggcaatccca taacgctggc ctcgtcgata caagagtact
      421 ggttggtaac tcgaatatgc ggcctgcaat ctcatgttcg gctcgtcacc aagatcgagc
      481 gcgagaattc gactgccggt cccaaggaaa gggtgattga gccgaatcac ctccatgccg
      541 ttgatggcga acccttctgg tcgatgcccc cgtgcctgat gttcgaaatc gaggaggagc
      601 tccagaatcc gtggctcggt ggccccagtc ggcaaacgtt tcagcgcttc aagaaaggca
      661 tcataatcga cggcgtccga aacaaccgat cgctgcagca gttcaggcga tacccgcaac
      721 gcccgtaact gacgcagcac gtcgagtcga atgcgcgcgg ggtgtcgcag gaacaccagg
      781 ctgttcgtcg gaacctgctc ggctatcacc gaagccaggt cggcgcggat gtgccagaaa
      841 tctggtgcca gaatggccca ccagctctga gtcatcacca gaatcccgac accgatggtc
      901 agtcgcagca cccgtgatcc ggctcgggga tgaatggtgt cgagctgccc cagcagacgc
      961 gccgctcccc aggccgcggc cggcagcaag ggaaacagat atcggggacc atagcagaaa
     1021 tcttgaaaat agtaccagct gtatccgaga atcagagcgg tggcatgcag gcaaaagaag
     1081 gtctcaattg ttcgacagac ctgcgactca agtgtcacgc gctctcgcaa cagtgcgaat
     1141 ccggcccaac cagccagccc accgccaaag gcccaccccg caaaacccag caggttgtcg
     1201 agattgttcg accagagcag acccggcgtc agctgctgca gaatcgtcgc cgacgcctga
     1261 acatggcgca ccgccagcag gggatcacca gcgattgcca cagcccaccg tgactgcacc
     1321 agagctgcca caacgcccgc cccggtgatc agcgcagacc cacccagccg aaccgcccag
     1381 ccttccgcaa tcaggaacaa gctgacgcag agcgacaacg ccagagctgc cccttccacc
     1441 cggcgggtca gcagcaagag actcacggca accgaggcca gcagaatgcc gcgccaccga
     1501 cgcttcgcca cgaactccgc caacccgttg gccgccagca acagacaaca caggccgagc
     1561 agatgattca tgcgagaggc gctcatcaac agcaggaacg gagaacacgc gaagagaacg
     1621 accgcatagc ggttcacatc aaaacgtggg acctgacgac acacacgaga caacaacagc
     1681 aggcacagga cgcccaggag ggggttcaac agaacgtcca ggttcacgat ctggaatagt
     1741 gccttcagaa gggcatatgc gggttgatag gttgaataga tccaggtgcc attctcgtca
     1801 atcgatgggt cggggagaaa cgactcgcgt actggtaatg gtggtgtatg aagagctcca
     1861 agacgaaaca ggcgcgactg ccattcctgt atgatctcgt cctgtacccg cggaattcct
     1921 ttcaggaggg cgacctgccc aatgaggccg caggtcaaaa tcaggagtcc cagccagaaa
     1981 atggatgaac gtcgaggcgt ggcaatgtca gacggccccg gttgtcgtgc cgcgagctct
     2041 tcttccggtg gggcggcaca gcggcgcaac cagatcaacg ccgcgaaggt ccgactccag
     2101 tcaagcccca gcaacaacag cgacaagaca ctttccggct ggatacaggt gtatccgctg
     2161 ttggtatcca tcagacgctg cggcagcatc agcaatgccg ccagaagcca gagacgccaa
     2221 cccaggcgtt gtgagagtgc cgcaagcaga gtacgccact gctttttctc ttgttcgttg
     2281 ccgacggcgc gaacaaccgt cagggccaga aataccagaa cgactccgat ccagagatcg
     2341 gaagacatgc caacaacacc cgccaggtca gagaggcgca tatcccagcg aatggcatca
     2401 gactggatca acgcggttcc cacatgcgcc aggatcaaga gcagcgcatc gatcaggatg
     2461 agaatcatga agttcctcga ggtgacagcg actttcaggg agagtgatgg gcaaacatga
     2521 tggatcagcc acattctgcc gatacagaag agtatacgat gtccgtatca tctgtcactt
     2581 ctctgcgacc gaatgccggt ctcgcgccga tcgcgc
//
LOCUS       DAORHD010000681         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46882_length_2616_cov_3.215931, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000681 DAORHD010000000
VERSION     DAORHD010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46882_length_2616_cov_3.215931"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKO06_13975"
     CDS             <1..882
                     /locus_tag="PKO06_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HNV70804.1"
                     /translation="EHHEKVMSRMRKDVFPFIGGKSLSDVTPQDLLNCARRVVERNAI
                     ETAHRMVRECGQVFQYAIITGRCSFNPAQGLTKGLPGYRGLSKNFAAFTKPDDVARLL
                     RIIESISAGPVVRAGLKLVSLLFVRSKELRMAEWKDIDFEAAEWRYRVTKNKNVKTLH
                     VVPLSWQALAILRELHPLTGHGRYVFPSGRTPDGSRPMSNMALLAALRSAGVTKEETT
                     IHGFRAMAQTMLHERLHFDRHIIEIQLTHKEMRGSLGTAYDRTEFLDDRRRMMQEWAD
                     YLDGLRAGSVRPAGAVA"
     gene            1029..1586
                     /locus_tag="PKO06_13980"
     CDS             1029..1586
                     /locus_tag="PKO06_13980"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HNV70805.1"
                     /translation="MERLEHAICFFAREFKKQAKKPLQSMGLYKLLALLEFRLLKKTG
                     RPVFGLDYLAMRMGPVPVSLYEKRNQVHSRLFSFTEQGENRFSVDPIGNPDMDYFSGV
                     EVAEMRRLVEIFSDPQMTTALFSEASHQEIMAWSRAYRKQPNSPIDFKDEFPGDIDTK
                     KAEDLTPEEESFLFWRGAQILNAAG"
     gene            1573..2025
                     /locus_tag="PKO06_13985"
     CDS             1573..2025
                     /locus_tag="PKO06_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70806.1"
                     /translation="MQPGEVVRWANFPFQKEGPKKARWLICFGTVKFYGQETQVYLAT
                     TTTKLEYYQPGGKRRNQPHYLIKKSLAPTFDADCVIDFMLDAPQPFLESCLTRANQDI
                     ESKGVLSNPVMSEMFSALVKSECVPPKILRELRSSLEVCGYKNLAKVK"
     gene            2025..>2616
                     /locus_tag="PKO06_13990"
     CDS             2025..>2616
                     /locus_tag="PKO06_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HNV70807.1"
                     /translation="MNLESTALVVFKGKQIRRVLNGGEWFFAVVDIVAALTDSLNPAD
                     YLKKMRARDEALSEGWGQIVTPLEIPTPGGKQRLNCANAEGLFRIIQSIPSPKAEPFK
                     RWLAKVGYERVQEIENPELASKRARALYKAKGYPDAWIEKRIRSIAIREELTDEWKNR
                     GVGEDREYAILTAEISKATFGLTPSEYSKVKGLKRQN"
BASE COUNT          629 a          638 c          774 g          575 t
ORIGIN      
        1 gaacatcacg aaaaggtgat gagccgcatg agaaaagatg tgttcccttt catcggcgga
       61 aagtcgcttt cggacgtgac cccgcaagac ctgctgaatt gtgcccggcg ggtggttgag
      121 cggaacgcga tcgagaccgc ccaccggatg gtcagagagt gcgggcaggt gtttcagtat
      181 gcgatcatca ccgggcggtg cagcttcaac ccggcgcaag gtctgacgaa aggcctgccg
      241 gggtataggg gcctgtcaaa gaacttcgcg gcgttcacca agcccgatga cgtggcgcgg
      301 ttgctgcgga tcatcgagag catatcagcc gggccggtgg tgcgtgcggg tctcaagctc
      361 gtgtcgctgc tgtttgtccg gtcgaaagaa ttgagaatgg ctgaatggaa ggacatagat
      421 ttcgaggcgg ccgagtggcg gtatcgagtg acgaaaaaca agaacgtgaa gaccctgcac
      481 gttgtccctc tgtcctggca agcgttggcg attcttcgcg aactgcaccc ccttaccggt
      541 catggacggt atgtattccc cagtggccga actcccgatg gttccagacc catgagcaat
      601 atggcgcttt tggcggcttt gcggtctgcc ggggtgacaa aggaagagac gaccattcac
      661 ggtttccggg ctatggctca aaccatgctg catgagcggc ttcacttcga ccggcatatc
      721 atcgaaattc agctgaccca caaggaaatg aggggttccc tcggaacagc ctacgaccgc
      781 acagagtttc tcgacgatcg gcggcgcatg atgcaggaat gggccgatta cctcgacggg
      841 ctccgggccg gttccgttcg cccggctggt gctgtcgcct gaagtattgg cgcggatcgg
      901 tgcgagggtg tgagtaaaac ggcgggggaa tctactcaca tgggtggtgc atgtgtgagt
      961 aaaccggtgg agaatgaatt gcatacctca tgttcggtag gtatatttac gttatgagac
     1021 agcatgatat ggagagattg gagcacgcaa tctgcttctt cgcccgtgaa ttcaagaaac
     1081 aggccaaaaa gcctttgcaa tcaatgggcc tgtacaagct tcttgcgctt ctcgagttcc
     1141 gtctgctcaa aaaaacgggc cgtccagttt ttgggttgga ttacttggct atgagaatgg
     1201 ggcctgtgcc ggtgtcgttg tatgaaaaac gcaaccaggt tcatagccgt cttttctcat
     1261 ttaccgaaca gggggagaac cgatttagtg tcgatcccat cggaaatcct gatatggatt
     1321 acttttcagg tgtcgaagtt gcggaaatga ggcgtctggt agagattttc tccgaccctc
     1381 agatgactac tgcgctgttc agtgaagcat ctcaccaaga aatcatggct tggagtcgcg
     1441 catatcgcaa acaacccaac tcgcccatcg acttcaaaga cgagtttcct ggtgatatcg
     1501 acacgaaaaa agcggaagac ctgaccccag aggaagaaag tttcctcttc tggcgtggcg
     1561 ctcagatttt aaatgcagcc gggtgaggtt gtacgttggg ccaacttccc ttttcagaag
     1621 gaagggccga aaaaagcacg ctggctcatc tgcttcggca cagttaagtt ttacggtcaa
     1681 gaaactcaag tctacttggc caccacaaca acgaagctgg aatactacca gcccggcgga
     1741 aaacgccgaa atcagccgca ttacctgata aagaagagcc ttgcgccgac gtttgatgct
     1801 gattgcgtga tagatttcat gcttgatgcc ccgcaacctt tcctggaatc ctgccttacc
     1861 cgggcgaatc aagatattga atcaaagggg gttctatcga atccggtcat gtccgaaatg
     1921 ttttcagctt tggtgaagtc tgagtgcgtg ccgccgaaga ttctacgcga gttgcgtagt
     1981 tctctggaag tctgtggata caagaatctt gcgaaggtga agtaatgaac ctggaatcaa
     2041 cggctctggt ggttttcaag ggcaagcaga tacggcgcgt gttgaacggt ggggagtggt
     2101 tcttcgctgt ggtcgatatt gtggcggctc tgacggactc tctgaaccct gccgactatc
     2161 tcaagaagat gagagcccgt gacgaagccc tttccgaagg gtggggacaa attgtcaccc
     2221 cccttgaaat accgacgccg ggcgggaaac agaggctcaa ctgtgcgaac gctgaggggc
     2281 ttttccgcat cattcaatca atccccagtc cgaaggcgga accgttcaag cggtggcttg
     2341 cgaaggtggg gtatgagcgg gttcaggaaa tcgagaaccc ggaacttgca tcgaaacggg
     2401 caagagcgct ctacaaggcg aagggctacc ccgatgcctg gattgagaag cggatacgct
     2461 ctatcgcaat tcgggaagaa ctgaccgacg aatggaaaaa tcgaggtgtc ggggaggatc
     2521 gagaatacgc catactcacg gcggagatta gtaaagcaac cttcggcctg accccctctg
     2581 aatactcgaa ggtgaagggg ttgaagcggc agaacc
//
LOCUS       DAORHD010000682         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46899_length_2615_cov_3.930859, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000682 DAORHD010000000
VERSION     DAORHD010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46899_length_2615_cov_3.930859"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKO06_13995"
     CDS             complement(<1..574)
                     /locus_tag="PKO06_13995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV70808.1"
                     /translation="MRVKSVMVIGCLILAGSGLLWAAESVTNNEATQKPEAAVAAPES
                     PAKNDSAPSETPAAPQKLTLDECLKIALERNHRQPASKFAVEAADAQLRQVKAGYMPQ
                     FLARSTYLRQDEPGNFIFPEESSFYQVKGLDALLPPGVPAGTPIPLNVTIPEKDVKLG
                     DRTSWVNEVGMKFPLYTGGQLEGLTKQARAG"
     gene            798..2054
                     /locus_tag="PKO06_14000"
     CDS             798..2054
                     /locus_tag="PKO06_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HNV70809.1"
                     /translation="MSTSFAEYPFHLELSTQGHLIDSGIMSSIMNLIIRSGGAYQILE
                     FTMGKSHDEYSKARFVVYARTEAQLRRIQEGLYEFGCQTQEEPNACLKAAEKDWVVPD
                     EFYSSTNHRTEVKLDGHWVEVANQRMDAMLVCRQEPDGWKVVCRKLRQIKAGDLVVCG
                     YQGIRIHPEFKGRDLEEFGFMNSDVSSERRVRIAVNKIARILLEKRKQGKRIGVTAGP
                     VVVHTGGAEYLAGLIRDGFIDFMLAGNAVAVHDIEQNLYKTSLGIDIHSGDPVSEGHR
                     NHMRAINAVFRHGSIKAAVETGTITSGIMYELTKRNLPYVLAGSIRDDGPLPEVVTDM
                     IAAQEAYARELKDCGMMLLLSSMLHGIGVGNMLPGWVKTICVDINPAVVTKLADRGSH
                     QTIGIVTDVGTFLASLHQELKALTQS"
     gene            complement(2120..2389)
                     /locus_tag="PKO06_14005"
     CDS             complement(2120..2389)
                     /locus_tag="PKO06_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70810.1"
                     /translation="MFIGLVLFVTISLIVFGAAQAAFLRHTGRLFWRAMLPLAWRRAM
                     LPNDEVTLDEFGRLEEWTALLMAMAFLTLPFIALNFIGFLNILPG"
BASE COUNT          580 a          774 c          750 g          511 t
ORIGIN      
        1 gaccggcacg cgcctgtttg gtcaagcctt cgagctggcc accggtatag agcggaaact
       61 tcatgccgac ttcgttgacc caactggtac gatcaccgag cttcacatct ttttcaggga
      121 tggtgacatt cagcggaatc ggcgtgcccg ctggaactcc aggcggcaga agcgcatcga
      181 gccccttcac ctgatagaac gaagactcct cggggaaaat aaaattcccg ggttcatcct
      241 gccgcaggta ggtcgagcgg gcgaggaact gtggcatgta gccggctttc acctggcgca
      301 actgcgcgtc agcggcttcg acggcgaact tcgaggcggg ctggcgatgg ttacgttcca
      361 gagcgatctt caagcattcg tcgagtgtca gcttctgtgg cgcggcggga gtctcggacg
      421 gcgcagagtc attctttgcc ggtgattccg gtgcggcaac ggccgcttca ggcttttgag
      481 tcgcctcatt gttggtaacg gattcagctg cccacagcag ccccgacccc gcgaggatca
      541 aacaaccgat gaccataaca cttttcacgc gcatagttca gcaacctcgt ttctgacgag
      601 aagttccggc tcaccgtaaa acaagacgaa ccggggggat gaagcgtcat catacaccgc
      661 gcgaaccata ccgtctactc agaagaattc ttattgcggt agctctgttc acagaatcga
      721 accggtggta gaatcgcccg ttcgtgtgag acagcgtcag gcgtgaccat cgccgatccc
      781 cccgaaggag acctcgtatg agcacctcat ttgccgaata cccgttccac ctcgaactga
      841 gcacccaggg gcatctgatc gactcaggca tcatgtcgtc gatcatgaac ctcatcattc
      901 gcagcggcgg ggcgtatcag attctcgagt tcacgatggg aaagtcccac gatgagtatt
      961 caaaagctcg cttcgtggtg tacgcccgca ccgaggcgca actgcggcgg attcaggaag
     1021 gcctgtatga gttcggctgc cagacgcagg aagaacccaa cgcctgtctg aaagccgctg
     1081 agaaagactg ggtggtgcct gacgagttct actcatcaac gaatcaccgc accgaggtca
     1141 aactcgacgg acactgggtc gaggtcgcga accaacgcat ggatgccatg ctcgtctgca
     1201 ggcaggaacc cgacggctgg aaggtcgtct gccggaaact gcgtcagatc aaagccggcg
     1261 atctcgtcgt ctgcggctat cagggcattc gcattcaccc cgagttcaag gggcgcgatc
     1321 tcgaagagtt cggtttcatg aacagcgatg tcagttctga acgccgggtg cgtatcgccg
     1381 tgaacaagat cgcgcgcatt ctgctcgaga agcgcaaaca gggcaagcgc atcggggtca
     1441 ctgccggccc ggtcgtggtg cataccggag gggccgagta tctggccggt ctgattcgcg
     1501 acggattcat cgatttcatg ctcgccggca acgccgttgc ggttcatgac atcgagcaga
     1561 acttgtacaa aacctctctc ggcatcgaca ttcattccgg agacccggtt tcagaagggc
     1621 atcgcaacca catgcgggcc atcaacgccg tctttcggca cggatcgatc aaggccgccg
     1681 tcgagaccgg aaccatcact tccggcatca tgtatgaact cacgaagcgg aatctgccgt
     1741 atgtgctcgc gggcagcatt cgcgatgacg gccctctgcc cgaagtcgtc accgacatga
     1801 tcgccgccca ggaagcctac gcccgcgagc tcaaagactg cggcatgatg ctgctgctga
     1861 gttcgatgct gcatggcatc ggcgttggga acatgctgcc cggctgggtc aaaacgatct
     1921 gcgtcgacat taacccggcg gtcgtgacga agctggctga tcgcggctcg catcagacga
     1981 tcgggatcgt gaccgacgtc ggcacgtttc tggcctcatt gcatcaggaa ctgaaagccc
     2041 tgacccagag ctgaagtcac tcgctcataa cccggacaga cgtcggttgt tcccgggtcg
     2101 tttctgtgcg gctcgcgaac taccccggca gaatgttcaa aaagccgatg aagttgagag
     2161 caatgaatgg cagggtgaga aacgccatgg ccatcagcag cgccgtccat tcttccagac
     2221 ggccaaactc gtcgagggtc acctcatcgt tcggcaacat ggcccgtcgc caggccaacg
     2281 gcagcatggc acgccagaaa agacgccccg tgtgccgtaa aaacgcggcc tgcgccgctc
     2341 cgaacacgat cagcgaaatg gtgacaaaca gaacgagacc gatgaacatg gatgtcctcc
     2401 atgagtttca tcggcatttt cgttccggtt ttccagcggg ggcgtcacga atcggttgcg
     2461 cgcggtttcc aggaaaagac cgccagagtg atatcgtctt cgagcggacg accagcggca
     2521 aaccctctga ccgcctgttg caccccagaa atcaaggttt ccggacttct ctccgacaac
     2581 tcctgtaaca tgcgttgaaa gccttccagg ctgac
//
LOCUS       DAORHD010000683         2612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_46997_length_2612_cov_3.256160, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000683 DAORHD010000000
VERSION     DAORHD010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46997_length_2612_cov_3.256160"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..2155
                     /locus_tag="PKO06_14010"
     CDS             107..2155
                     /locus_tag="PKO06_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70811.1"
                     /translation="MPDYAAPYVLVCYDPVELEEFLFHLHAVERTELAGAASPVYHGM
                     FGGLSCRVVRLPFGDRLECLQRVAAELCRRYRPRTLFSLGTAMALSPALSRGQVVIST
                     GAVFHGQTREFGAEHRRAIECARPHGVSLSYCRSLTTDSFVQTAEQGRALLEKVPEAA
                     IIEMEDFHLAEAAARAQIPFFSLRVVTDRGSFEEHMAALRDAARTSIRLYQRLFRQLT
                     MERWLNQEENPNTAELAFRILVRSGKGTLPIPQAVRLARNLCRQFRLRTMLPRDAMLD
                     LVIAPPGEWKGLTATPSGQANVWQPGEQWLFIHDPQTFFTRIVFPRENLAIHFHADQP
                     PVPVLGRLETRLVTKQAFPEGRLYELPRLDKLHIQGLNSISAEPEEGLLAAATIEEAR
                     AADLEVVHADEARIWLYRTANVSESSYFQTQYEFPYRSLRPGTSGEFCIATGIGPMVD
                     PGAEGGRDAQMLLLGDELCGRWLQILEDLAASPFHLTDAARYDGLILRLEPGVVLLSR
                     ADRRAYLNESGLNALTLEPLALRKIYSPAYDRFLRQYFGASRRSEPAVTSHLFLTSRG
                     CGSGCSICCSGGFQPFSALSAEQVSRGLGRFIERENLQPGQYIDVFLLDSNFNRDPNR
                     VISLANQLDRDNILPYVELYIRHNALSPFLLKKARDHAPTIVHRDLIRAYRRLGRFAR
                     "
     gene            2121..>2612
                     /locus_tag="PKO06_14015"
     CDS             2121..>2612
                     /locus_tag="PKO06_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70812.1"
                     /translation="MRIVGWDDLLDEEHYQGFPPEFFSVPGDVVESLDGDDLTGEFRA
                     FIHGPFKQGSDEDVGDRNDTSLAMQIVFGDDPSAGGVLLFGDHKYPVIRKIFDITKAH
                     GNEADLRWQVLLMRPVKNTPEFKGRLELVVAGTRDGKSWSQTLSGEGQALQLRQYRRM
                     EGVV"
BASE COUNT          509 a          728 c          784 g          591 t
ORIGIN      
        1 ttggtgactg tatcgtaaaa accgtcagaa agcttgaatc tcggcgttga attgtctaca
       61 ttctacgtgc acgcaccgct tcacagcaac aggggagact gaacccgtgc ccgattatgc
      121 cgcgccatat gtgctggtct gctatgaccc tgttgaactc gaagaattcc tgtttcatct
      181 gcatgcggtc gaacgaaccg aacttgccgg ggcggcgtcg cctgtctacc atggaatgtt
      241 cggaggtctc agttgccggg tggtgcgtct gccgttcggt gaccgcctcg aatgtctgca
      301 acgggttgcg gcagagctgt gtcggcgtta tcgaccgcga acgctgttct ctcttggaac
      361 cgccatggcg ctctcgccgg cactttcgcg tggtcaggtg gtgatcagca ccggtgcggt
      421 gttccacgga caaacccggg agttcggggc ggaacatcgg cgggcaatcg aatgcgcccg
      481 cccccacggg gtgagccttt cctactgtcg ttctctgacg acggattcgt ttgtccagac
      541 tgccgaacag ggaagagccc tgctggaaaa ggttcctgaa gcagcgatca tcgagatgga
      601 agatttccat ctggcggagg ctgcagccag agcgcaaatt cctttcttct cccttcgtgt
      661 ggtgaccgac cgcggctcat tcgaagaaca catggctgcg ctcagagatg cggcgagaac
      721 atccattcgt ttgtaccaac gactgtttcg acagcttacg atggaaagat ggttgaatca
      781 ggaagaaaac ccgaatactg cggagctggc atttcgcatt ctcgtgcgct ccggcaaagg
      841 aacactgccg attccccagg cggtgcgatt ggcgcgcaac ctctgccggc agtttcggtt
      901 gcgcacgatg cttccacgtg atgcaatgct tgatctcgtc atcgctcccc cgggagaatg
      961 gaaggggttg acggcaacgc catcgggtca ggcgaacgtc tggcagccgg gtgaacagtg
     1021 gctgttcatt cacgatccac agaccttctt cacccgcatt gtctttcctc gcgagaacct
     1081 ggcgattcac tttcacgcag atcaaccgcc ggtacccgtt cttggtcgcc tggaaacgcg
     1141 tctggtcacg aagcaggctt ttcctgaggg acgattgtat gaactgccgc gcctggacaa
     1201 gctacacata caggggctga actcgatctc cgcggagcct gaagagggcc tgttagcggc
     1261 cgccacgatt gaagaggctc gtgctgccga tctcgaagtt gttcatgctg atgaagcccg
     1321 catctggctc tatcgcacgg cgaacgtctc tgagtcctcg tatttccaga cgcagtatga
     1381 gttcccgtat cgatcactgc gcccggggac atccggggag ttctgcattg ccaccggcat
     1441 cggtccgatg gttgatccgg gggcagaagg cgggcgtgat gcgcagatgt tgctgcttgg
     1501 tgatgaactg tgcgggcgat ggttgcagat cctcgaagac ctggcggcgt ccccgttcca
     1561 cctgaccgat gccgcgcgct acgatggctt gattctacgt cttgaacccg gggtggtact
     1621 gctaagtcgg gccgatcgcc gcgcgtatct caacgagtcc gggttgaatg cgcttactct
     1681 tgagccactg gctttgcgca agatctattc tccggcctat gatcgttttc tgcgacagta
     1741 tttcggagca tcacgacggt ctgagccggc ggtaacgtca cacctctttc ttacgagtcg
     1801 tgggtgtggg agcggttgca gcatctgctg ttcaggaggg tttcaaccct tctcggctct
     1861 ttcggctgag caggtttccc gaggcctcgg tcgtttcatt gagcgcgaaa acctgcaacc
     1921 tggccagtac atcgatgtgt ttctgctcga cagcaacttc aatcgcgatc cgaatcgtgt
     1981 catcagccta gcgaatcaac ttgatcgaga caatattctg ccctatgtcg agttgtacat
     2041 tcgtcacaat gccctaagcc cgtttctgtt gaaaaaagct cgcgatcatg ctcccacgat
     2101 agtgcaccga gatctgatcc gtgcgtatcg tcggctggga cgatttgcta gatgaggaac
     2161 actatcaggg attcccgccc gagttcttca gcgtgccggg cgacgtcgtg gaatcgctcg
     2221 atggcgatga cctcacaggt gaattccgcg cgttcatcca cggcccgttc aagcaaggct
     2281 cggacgaaga cgttggagat cggaacgata cgagcctggc catgcagatc gtgttcgggg
     2341 atgatccttc ggcgggagga gtgttgctct ttggcgacca caagtacccc gtcatccgga
     2401 agatcttcga tatcacgaag gcgcacggga acgaggcgga cctgcggtgg caggtgctgc
     2461 tgatgcgccc tgtcaagaat acgcctgaat tcaaggggcg tctggagctg gtggtggcgg
     2521 gcacccggga tggcaaatcc tggagccaga ccctgagcgg cgaagggcag gcgctgcaat
     2581 tgcgccaata ccggcgcatg gagggcgtgg tc
//
LOCUS       DAORHD010000684         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47011_length_2611_cov_4.878326, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000684 DAORHD010000000
VERSION     DAORHD010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47011_length_2611_cov_4.878326"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKO06_14020"
     CDS             complement(<1..546)
                     /locus_tag="PKO06_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70813.1"
                     /translation="MMRREVGASLTTLLYRTHRWQTGAGTRRRPGFTVFLLNTGFFAI
                     LLLTPYLLFQLHFRDTANQVARQERERTLRRLKQDQQLVERMLDPRMQLSLRLQSILF
                     EAQRRYLSPERPPRESLQRMHRHLRQRLAPLARLVPSLRWQMLEYQPYYSGCRVEGGY
                     YWYGYNSPWNFAADVEGFPFLL"
     gene            complement(543..>2611)
                     /locus_tag="PKO06_14025"
     CDS             complement(543..>2611)
                     /locus_tag="PKO06_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70814.1"
                     /translation="NVEVLGEVRLLTSLIGRFEALGKWWFRVCPLHPQAGKVGVLCLP
                     QPPPSMPSRERLARILLGLWLIGWGVVLVYRLLTGEMPIPGVRLGLVMWFLALVSVPT
                     MLSISTSASFLSDLESNLHHQKRQEMMQTFREVEAGSTSLGERYSVQLRRLTQNRQLC
                     QQLLDAQHGKGNVEQVMQTLWQETEKQEIELMAVLVFGHGGFTQNVFHKTVTPEVQRA
                     FQQFVGEFGDTTLRQADPELYEKEKARMLASGQSRPKRGQFEGLASSSFLLTQMDSSA
                     DFRMGRRHLMKYHNSLGFDGHTYFSIFFIWDQDPLYRRYLETAIPEANRRYDWLSLGA
                     FRDQGDQLETIWKPRLGEDLTTWAEQAREQVIWDRGRDRLLYAYPSKRMPGYVLAATG
                     SLHAVQDAIRQEVQRIVFTLIFLVLLVGLSSAGLSHWLASPILRMTEGLRQVAHGNLQ
                     IQVAENRDDELGEAGTTLDTMTGWLRERRLMTRFVAPQVLELIQSGTMDQNCSATTQE
                     AVALVSDIRNFTTLSETHPPAEVFAALNYHLQVMTRVIREQNGYIDRFIGDAIQAVFY
                     PGDVPAAVRAVRAAAGMRRGHHLIQVHRERQKKFVYEIGIGIDCGVLVTGVLGDPELR
                     QEYSVLGEPMKHAAELEALSKLGTATRIICSTPVVHDCGLQDHFRHLEGHENCWELVK
                     LPEARS"
BASE COUNT          561 a          743 c          807 g          500 t
ORIGIN      
        1 cagcagaaag ggaaacccct ccacgtccgc ggcaaaattc cagggagagt tgtaaccata
       61 ccagtaataa cctccttcaa cgcgacatcc ggaataatat ggctggtact caagcatctg
      121 ccagcggagc gaagggacaa gacgggccag aggcgcgagt cgctggcgca ggtggcgatg
      181 catacgttga agactctctc gaggaggacg ttccggtgac agatatcggc gctgggcctc
      241 gaacagaatg ctttgcagcc gcaggctcaa ctgcatgcgt ggatcgagca tccgctcgac
      301 cagctgctgg tcttgtttca gacggcgcag ggttctctcc cgttcctgtc tggcgacctg
      361 attcgccgta tcgcgaaagt gaagctggaa gagcagatac ggagtgagca ggagaatggc
      421 aaagaagccg gtgttcagca gaaagacggt gaagcctggt cgtcgccggg ttccggctcc
      481 ggtctgccag cgatgcgtcc gatagagcag cgtggtgagc gaggcaccga cttcacgtcg
      541 catcacgatc tggcctccgg cagcttgacc agctcccagc agttctcgtg tccttcgagg
      601 tgccggaaat gatcctgcag gccgcagtcg tgaacgacgg gagtcgagca gatgatgcga
      661 gtggccgtgc cgagcttcga cagtgcctcc agctcggcgg catgtttcat cggttcgcca
      721 agaacgctgt attcctgccg cagttccggg tcgccgagaa cgccggtcac gagaaccccg
      781 cagtcgatgc cgatgccgat ctcgtagaca aacttcttct gtcgctcgcg gtggacctga
      841 atgaggtggt gtccgcgcct catgccggct gccgcacgaa cggcccgcac ggcagcggga
      901 acgtcgccag ggtagaagac cgcctgaatc gcatcaccga tgaaacgatc tatgtagccg
      961 ttctgttcgc gtatgacccg cgtcatgact tgcagatggt agttcagggc ggcgaagacc
     1021 tcggccggtg gatgggtttc agagagggtc gtgaaattgc gaatgtcaga aaccagagcg
     1081 actgcttcct gcgtggttgc tgaacaattc tgatccatgg tgccgctctg aatcagttcc
     1141 agaacctgtg gcgccacgaa ccgggtcatc aaacgtcttt cgcgaagcca gccggtcatg
     1201 gtgtccaggg tggtgccggc ttctcccagc tcatcatcgc ggttttcggc tacctgaatc
     1261 tgaaggtttc cgtgagcgac ctgtcgcaac ccctcggtca tgcgcagaat cgggcttgcc
     1321 agccagtggg aaagaccggc gctgctcaaa ccgaccagca gcaccagaaa gatcagggtg
     1381 aagacaatgc gctgcacttc ctggcggatg gcgtcttgga ccgcatgcaa cgaaccggtg
     1441 gcggcgagta catatccggg catgcgcttg ctggggtagg cgtagagaag tcggtcacgc
     1501 ccgcggtccc agatgacctg ttctcgcgcc tgctcggccc aggttgttag atcttcgccc
     1561 agacgcggct tccagatggt ttccagttgg tcgccctggt cgcggaaagc gccgagggaa
     1621 agccagtcat accggcggtt ggcttcggga atggcggttt ccagataacg acggtagagc
     1681 gggtcctggt cccagatgaa gaagatcgaa aaataggtgt ggccatcgaa tcccagagag
     1741 ttgtgatact tcatcagatg gcgacgaccc atgcgaaagt cggcggaaga atccatctga
     1801 gtcagaagaa aggacgagga agcgagaccc tcgaactgac cacgctttgg tcgagactgg
     1861 cctgaagcca gcattctcgc cttttccttc tcgtacagtt ccgggtccgc ctgacgcagg
     1921 gtcgtatcac cgaactcgcc gacaaactgc tgaaacgctc gctgaacctc gggagtgacc
     1981 gttttatgaa agacattctg agtgaagccg ccgtggccga agaccagcac ggccatcagc
     2041 tcgatctcct gcttttccgt ttcttgccag agtgtctgca tgacctgctc gacgttccct
     2101 tttccgtgct gagcgtcgag caattgctgg cagagttggc ggttctgcgt gagcctgcga
     2161 agctgaacgc tgtaccgttc tcccagagac gtgctgccgg cttcgacctc gcgaaaggtc
     2221 tgcatcatct cctgtcgctt ctgatgatga aggttcgact cgagatcgct gagaaacgaa
     2281 gcgctggtgc tgatactcag catcgtggga acggaaacga gcgctagaaa ccacatgacc
     2341 agacccagac gaacgccggg tatgggcatc tcgccggtca gcaggcgata gacgagcacc
     2401 acgccccaac cgatgagcca cagaccaagc agaatccgcg ccaatcgttc tcgggaaggc
     2461 atggaggggg gaggttgagg caggcagaga actccaacct tgccggcctg ggggtgcagg
     2521 ggacagacac ggaaccacca cttgccgagg gcctcgaacc gaccgatgag tgaggtcagc
     2581 agacgcacct cgccgagcac ttcgacgttg g
//
LOCUS       DAORHD010000685         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47013_length_2611_cov_3.738654, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000685 DAORHD010000000
VERSION     DAORHD010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47013_length_2611_cov_3.738654"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..939)
                     /gene="csm3"
                     /locus_tag="PKO06_14030"
     CDS             complement(193..939)
                     /gene="csm3"
                     /locus_tag="PKO06_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HNV70815.1"
                     /translation="MQNMKLQAIRQITGRIILKSGLHIGAGNMEMRIGGTDSPVIKHP
                     HTQEPYIPGSSVKGKVRSLVELAFGLVPFAHKDGGVTSLKTYRELEGKAELKKQCEKV
                     LKVFGYSPSDQEKDKELAADLGPTRVSFADCSLNKDWKTKAVDNHWALTEVKSENSID
                     RIQGAAQHPRFIERVPAGCEFDFRVSFRILEDADKELFNDLLSGLKLLELDALGGSGS
                     RGYGRIEFADLNVDGEPIKEKFDQIKPFAK"
     gene            complement(943..1410)
                     /gene="csm2"
                     /locus_tag="PKO06_14035"
     CDS             complement(943..1410)
                     /gene="csm2"
                     /locus_tag="PKO06_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HNV70816.1"
                     /translation="MQFQLMDKNSKIPVDLFCKDAEEIANEVYKGQQATRGKANKPTQ
                     IRKFYDEVVSFDTRLRSADEKTKNDEFQAILPYLKMLNAKAAYAEARELITREFRDFL
                     SQGLKQITTPQSFQVFVHLFEAFMGYYKYLDVKQEAAQGTVPPRTNNSQVGRK"
     gene            complement(1442..>2611)
                     /locus_tag="PKO06_14040"
     CDS             complement(1442..>2611)
                     /locus_tag="PKO06_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70817.1"
                     /translation="VPIFDRYQLSFRVSGRLSELIKAGTLLRYWDISHQSDGSLNRRF
                     TARPLKGYMPLTTEQDNAEDNVLIGRESDEAKLDLIKEEGTAKCFKAIAKACYFQRES
                     GEATGVEALGVLKADVDQLGAIMGFGLKKEMRTIARTVTISRFLNHFFSTYLPHVFQQ
                     AAEEKTVHGSQAEYRDIYTVFAGGDDLFLIGPWNHMITFASFLNKQFRAFTGENPELH
                     LSAGISIHHDQEPVTEMSEQAEEALEKSKKGDKTGDRNRVTIFGETATWTGFEKLREK
                     KTTIETWLRDKIINRAMLYRFNHVLQLAEHFQKMNQTEAIDFTCAEDIESIKWPAILK
                     YSIVRNVKEKKNHMEILKFAEWVGGTKRGAGDESFGSKLRMALWPVIYENRRARR"
BASE COUNT          564 a          736 c          636 g          675 t
ORIGIN      
        1 gagaccacca ggaacggttt cgtatcgtac gagtgcagca gatcgctcaa cggctttgac
       61 agcaatgttg agtcatactg aaactgccag cacaggtgac caaacaacgt gtcggccttc
      121 aggggagtgc caaaccccga ggttggtgat agagttatca ggaaggtttt cacggagacc
      181 tccttaggtc gactacttgg cgaaaggctt gatctggtcg aatttttcct tgatcggctc
      241 tccgtccacg ttgagatcgg cgaactcgat ccggccgtac ccacgactgc cactgccgcc
      301 gagcgcatcc aattcaagca acttgagtcc gctcaggagg tcattgaaaa gctccttgtc
      361 ggcatcttca agaatgcgga aagaaacccg gaaatcaaat tcacagcccg ccggcacgcg
      421 ttcgatgaaa cgcggatgct gagccgcgcc ctgaatgcga tcaatgctgt tttccgattt
      481 cacctcggtg agcgcccagt ggttgtcgac ggccttggtc ttccaatcct tattcaacga
      541 acagtcggca aacgacacgc gggtcggccc cagatcagca gccagttcct tgtctttctc
      601 ctggtctgac gggctgtatc cgaacacttt caagaccttc tcgcactgtt tcttcagctc
      661 tgccttcccc tcgagttcac gataggtctt cagagacgtc acgcccccat ctttatgagc
      721 gaatggcacg agaccaaacg ccagctcgac cagagagcga actttgccct tcactgaaga
      781 accggggatg tacggttcct gcgtgtgcgg atgtttgatc acggggctgt cggttccgcc
      841 gatgcgcatt tccatgttcc cggcaccgat atgcaagccg cttttgagaa tgatacgacc
      901 ggtaatctga cgaatcgcct gaagtttcat gttctgcata cgttatttcc tccccacctg
      961 cgagttattt gtgcgcggcg gaacagttcc ttgcgcagct tcctgcttta catcaaggta
     1021 tttgtaataa cccatgaacg cctcgaacaa atgcacaaag acctggaatg actgaggcgt
     1081 cgtaatctgc ttcagccctt gggataaaaa gtctcgaaac tctcttgtaa tcagttctct
     1141 ggcctcggca tacgcggctt tggcattgag catcttcaga tacggaagaa tcgcctggaa
     1201 ctcatcgttc ttcgtcttct catccgcact gcgaaggcgg gtgtcaaagc tcaccacctc
     1261 atcgtagaat ttgcgaatct gcgtgggctt gttcgctttc cctcgggttg cctgttgtcc
     1321 cttgtacact tcgttcgcga tctcctctgc gtccttgcag aacaggtcca cagggatctt
     1381 gctgttcttg tccatcagtt gaaactgcat gctcgattgc tcctttcaga gttatcaaaa
     1441 attatcgtct tgcacggcga ttctcgtaga tcaccggcca caacgccata cggagttttg
     1501 atccaaacga ttcatctcct gcacctcttt tcgttccacc gacccattcg gcaaacttca
     1561 gaatctccat gtgattcttc ttttccttga cgtttcgaac gatcgaatac ttgagaatcg
     1621 ccggccattt gatcgattca atgtcttctg cacaagtaaa gtcgattgct tccgtctggt
     1681 tcattttttg aaaatgctcg gccaactgca ggacgtgatt gaaacggtac aacatggcgc
     1741 ggttgataat cttgtctcgg agccaggttt cgatggtcgt ctttttctca cggagttttt
     1801 cgaagcctgt ccaggtcgcc gtttcgccga agatggtcac gcgattgcga tcaccggtct
     1861 tgtcgccctt cttcgacttt tcgagcgcct cctctgcctg ttcagacatc tcggtcaccg
     1921 gttcctggtc gtgatggatg ctgatgccgg cagacagatg cagttcggga ttctcaccgg
     1981 taaacgccct gaactgcttg ttgagaaagc tcgcaaatgt gatcatgtgg ttccaggggc
     2041 cgataagaaa gagatcgtca ccacccgcaa agaccgtgta gatgtcacgg tattcggcct
     2101 gagaaccgtg aaccgtcttt tcctcagccg cctgctgaaa gacatgcggc agatacgtcg
     2161 agaagaagtg gttgagaaac cgggagatcg tcaccgttcg cgcgatcgtt cgcatttcct
     2221 ttttcaaccc gaaacccatg atcgctccga gctgatcgac atcggctttc aacaccccca
     2281 gcgcctcgac gcccgttgcc tcgccggatt cacgttgaaa gtaacaggcc ttggcgatgg
     2341 ccttgaaaca tttcgctgtt ccctcttcct tgatgagatc tagcttcgcc tcatcgcttt
     2401 cacgaccaat gagaacattg tcttcagcat tgtcctgttc ggtcgtcaac ggcatatacc
     2461 ccttgagcgg ccgcgcggtg aatcgacgat tcagcgagcc gtctgactga tgcgagatat
     2521 cccagtatct gaggagcgtt cctgctttga tcaactcgga caaacgacca ctgacgcgga
     2581 agctcaactg atacctgtca aaaatcggga c
//
LOCUS       DAORHD010000686         2610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47042_length_2610_cov_3.758513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000686 DAORHD010000000
VERSION     DAORHD010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47042_length_2610_cov_3.758513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKO06_14045"
     CDS             <1..431
                     /locus_tag="PKO06_14045"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNV70818.1"
                     /translation="LMIVVLAVIGVSYWTSKIAAIGPAGELAAPPSEEEIAKAPKVGR
                     FSLGEFVATSRDEELHYIKIEVEVGYVGAIEKELEERKGELRDAVLTILMKMTIQRAK
                     EDYVDHFLHKDIEKKLNEVLGKTTSESRIVKVYIPQFLIN"
     gene            536..1642
                     /gene="fliM"
                     /locus_tag="PKO06_14050"
     CDS             536..1642
                     /gene="fliM"
                     /locus_tag="PKO06_14050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNV70819.1"
                     /translation="MVETLSQSEIDALLSALSTTGTSGGDAPPPPPSGAAESGGARPS
                     VVPGTSAPSAAATAVAMQRLADSDSKKKGKIYDFKRQTKFSKEQLGTIQLIHETFARL
                     VGTYLSTQLRAYAQATIISVDQLTFEEFIRSIYSPTFITGFRSEKLDGKALMDINLNI
                     VFTILDRLLGGSGTPLSTLRQLTEIEIQIMQRIVGKLLNALREAWINVVDLTPAIEFI
                     ESNPQFAQIVPPGDMVLSIVVEVKIHDVTGLMNLCIPYNTVEAIAPKLNAASWFAAIR
                     KEPLTKNVETITQQVRVVELPVIAELGTATLTLQDILNLQVGDILLIDRLVKEELDIR
                     VGNQIKFRGRPGVLGKKMAISINQVIQPDEEDKL"
     gene            1639..>2610
                     /locus_tag="PKO06_14055"
     CDS             1639..>2610
                     /locus_tag="PKO06_14055"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNV70820.1"
                     /translation="MTDLLSQEEIDALLRSAGSSDAASVEATPVAPAAAEPPQSAGGA
                     GAAAPPPPPPVAPPPAPPASPPPPSQPAGPEETLSQEAIDQLTEVAKLSLGATSSVVQ
                     MLLNRPIELVPSQLRVVSYSTLAGEGSTEDYVIAKIAYAEGFDGNSYLMIRKSISAII
                     GDLMMGGEGKSPEFQEMHKSAVGEMMNQMMGKTTTTLSELFKKRVDIAPPQVELVNFA
                     SGIPPLPDFHSNAHFLRIGFHLKIGDVAETEMVQLLPVKFAQVMAADLAKPQKAPEKA
                     KPAPPPPAAGAAQPAAAAAPAAGPGSPPPAAMPGMPGMGGMPGMMPGM"
BASE COUNT          574 a          809 c          729 g          498 t
ORIGIN      
        1 ttctgatgat cgtcgtcctg gcggttatcg gtgtctccta ctggaccagc aaaatagcag
       61 cgattggccc tgccggagaa ctggcggcac caccatccga agaagagatt gccaaagccc
      121 ccaaggtcgg tcgcttcagc ctcggcgagt tcgtcgccac atcgcgcgac gaagaactgc
      181 actacatcaa gatcgaggtc gaagtcggct atgtcggcgc cattgaaaaa gaattggaag
      241 aacgcaaggg cgaattgcgg gatgccgtac tcaccattct gatgaagatg acgattcagc
      301 gagccaagga agactatgtc gaccacttcc tgcacaagga catcgaaaag aagctgaacg
      361 aggttctggg gaaaacgaca tcggaaagcc gaatcgtcaa agtctacatt ccgcaatttc
      421 tcatcaacta gccctcttca caatcctcaa atcagccgat atcatagagg gcgggtattt
      481 ttcccgctcc cagccgggta atcaccccag ctgattttca ggaccgaggc ctgctttggt
      541 agagaccctt tctcagtctg agatcgacgc cctgctttcg gctctttcca cgacgggaac
      601 ctccggcggg gacgcaccac cgcccccacc atccggagca gccgaatccg gcggcgcacg
      661 cccgtccgtc gttccgggaa caagtgcgcc atcagccgcg gcgacggccg tggccatgca
      721 gcgtctggct gattctgatt cgaagaaaaa gggcaagatc tacgatttca agcgccagac
      781 gaagttttcc aaggaacagc tgggcacgat tcagctgatt catgaaacct tcgcccgcct
      841 ggtcggcacc tacctttcga cccagttacg cgcgtatgcg caggcaacga tcatctcggt
      901 cgaccagctg accttcgaag aattcatccg ttccatctac tcgccaacgt tcatcaccgg
      961 ctttcgctca gaaaagctcg atggcaaggc gttgatggac atcaacctca acatcgtgtt
     1021 cacgattctc gaccgtctgc tcggcggcag cggtacgcct ttgagcacgc tgcggcagct
     1081 caccgagatc gagattcaga tcatgcagcg cattgtcggc aagctgctga acgccttgcg
     1141 cgaagcctgg atcaatgtcg tcgatctgac gccggcgatc gagttcatcg aatcgaaccc
     1201 acagttcgcg cagatcgttc cgcctggtga catggtgctg agcatcgtcg tcgaggtcaa
     1261 gattcatgac gtgaccgggc tcatgaacct ctgtattccg tataacacgg tcgaggccat
     1321 tgccccgaaa ttgaatgcgg cctcgtggtt cgccgcgatt cgcaaagaac cactgacgaa
     1381 aaacgtcgaa accattacgc agcaggtgcg ggtggtcgag cttcctgtga tcgccgaact
     1441 gggaaccgcc acgctgaccc tgcaggatat tctgaacctt caggtgggcg acattctgct
     1501 gatcgaccgg ttggtgaaag aagaactgga cattcgggtc ggcaaccaga tcaagtttcg
     1561 cggccgcccg ggagttctcg gcaagaaaat ggcgatttcc atcaaccagg tgatccagcc
     1621 cgatgaggag gacaaactgt gacggacctg ctttcacaag aagaaattga tgcgttgcta
     1681 cgaagcgccg gctcatcaga tgccgcttcg gtagaagcaa ccccggtggc ccccgcggcc
     1741 gcggaacctc ctcagagtgc tggtggcgct ggggccgccg ctcccccccc gccgccaccg
     1801 gttgcgccgc cgccggcccc gcccgcgtcg ccgccacctc cgtcgcaacc cgccggtcct
     1861 gaggaaaccc tttctcagga agccatcgac caactgaccg aggtcgccaa gctatcactg
     1921 ggtgccactt cttcggtcgt tcagatgttg ctgaaccggc cgatcgagct ggttccgtcc
     1981 cagttgcgcg tggtcagtta ttcgactctc gccggtgaag gcagcaccga agactacgtc
     2041 atcgccaaga tcgcctacgc agaaggcttt gacggcaact cctacctgat gatccgcaaa
     2101 tccatctcgg ccatcatcgg agacctgatg atgggcggcg aaggcaaaag ccccgagttt
     2161 caggagatgc acaagtcggc cgtcggcgaa atgatgaatc agatgatggg gaaaaccacc
     2221 acgacgctgt cggagttgtt caagaaacgc gtcgacatcg cgccgcccca ggtcgagctg
     2281 gtgaacttcg cctccggaat tccgcccctg cctgactttc actccaatgc ccacttcctg
     2341 cgcatcggat ttcatctgaa aatcggcgat gtggcagaga cggaaatggt acagctgctg
     2401 ccggtcaagt tcgcgcaggt catggccgcc gacctggcga aaccgcagaa agctcctgag
     2461 aaagccaagc cggcaccacc accgccggct gccggcgcgg ctcagcccgc cgccgccgcg
     2521 gctccggccg ctggtcctgg gtcgccgcct ccggcagcga tgcccggaat gcctgggatg
     2581 ggaggtatgc ccggtatgat gcctggaatg
//
LOCUS       DAORHD010000687         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47095_length_2608_cov_4.711712, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000687 DAORHD010000000
VERSION     DAORHD010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47095_length_2608_cov_4.711712"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKO06_14060"
     CDS             <1..232
                     /locus_tag="PKO06_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HNV70821.1"
                     /translation="TWCPHCIRLLEFLRRERIPFQNFDVDEDDERWQEAMKLTGGIDI
                     VPVVEKRGEVVFGAFNSSFEKRLRDLLQLTAR"
     gene            264..1634
                     /gene="hisS"
                     /locus_tag="PKO06_14065"
     CDS             264..1634
                     /gene="hisS"
                     /locus_tag="PKO06_14065"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNV70822.1"
                     /translation="MINTAPPSGMRDFLPLETLRRRYVQNTIRRVFEQYGFVELETPA
                     VENLTTLLGKYGEEGDQLLFRLLHRRDRLARALEQETIAEKDLAEMGLRYDLTVPLAR
                     VIAHNSDLPRFFKRYQMQMVWRADRPGKGRYREFMQCDVDVTGTKSLIADTEVCSAVA
                     QALQELGLKDFTIQLNHRQLLKCLIKAAGIALEQEEVTLVAVDKWDKIGEPGVVQELQ
                     NRGIAAEAASRLLHLLKRPEGISETEALDRLSGLLHDIPEAEPAIQELRQVLALATHT
                     PAGSRLKVDATLARGLGYYTGPIFEIRSPLAKGSLGGGGRYDGLIGMFCNREIPAVGF
                     SIGFERVVLLLEEAGLFPTTGAGAHMLVCRFDDADPVEVMKAASILRQSGIKVEIYPE
                     TGKIGKQISYADTQKIPVVGIFGGAETAAQVISLKHLTSGEQAKLSYTESVAKIQEWL
                     QKTSTA"
     gene            complement(1719..>2608)
                     /locus_tag="PKO06_14070"
     CDS             complement(1719..>2608)
                     /locus_tag="PKO06_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV70823.1"
                     /translation="KTAEGYLKSGRYTWNSGMFVFHVETLLSELRKTCPELMKAFDGM
                     QISESEALRTAFGKSPKISIDYAVMEKTSKAAMLPSTFGWSDVGSWYSLFEVSPKDDA
                     GNVVVGNHVTIDTSNSLVIGRDRTIATLGVKDLAVIDTPDALLVCPLSESQRVREVVE
                     RLNQTQRREVREHLTVHRPWGSYTILEEGPRYKIKRIVVKPGKRLSLQRHQHRNEHWV
                     VVSGTATVRRENEELFVRENHSIYIQATQKHRLSNFGRIPLQIIEIQVGGYLEEDDIE
                     RYDDDWGRGDTSAARKQKK"
BASE COUNT          574 a          750 c          696 g          588 t
ORIGIN      
        1 cacctggtgt ccgcattgca tcaggctgct cgagtttctg cgccgtgagc gaattccatt
       61 tcagaatttc gatgtcgatg aagatgatga acgctggcag gaagccatga aacttaccgg
      121 cggcatcgat attgtaccgg ttgtcgaaaa gcgtggagaa gtggtcttcg gcgctttcaa
      181 cagttctttc gaaaaacgac tgcgtgatct gctgcagttg actgctcgct gaatccattt
      241 ccgaaaaatc acacaggaat cctatgatca atacagctcc acccagtggc atgcgcgact
      301 ttctgcctct cgagactctg cgccgaagat atgtgcaaaa caccattcga cgtgttttcg
      361 aacagtatgg gttcgtcgaa cttgaaactc ccgcggttga aaacctgacc accctgctgg
      421 gaaaatacgg cgaagaaggt gaccagttgc tgtttcgtct gctgcatcgt cgcgaccgac
      481 tggcgcgcgc cctcgagcag gaaacgatcg ccgagaaaga tctggcggaa atgggcctgc
      541 gctatgacct caccgtgccg ctggcgcggg ttatcgcgca caactccgat ttaccgcgtt
      601 tcttcaagcg ttaccagatg cagatggtct ggcgggcaga ccggcccggc aagggtcggt
      661 atcgcgaatt catgcagtgc gatgtcgacg tgaccggcac caaatcactt attgccgata
      721 ccgaagtctg ttcagccgtg gctcaagctc tgcaggaact gggactcaag gatttcacca
      781 ttcagctgaa ccaccgtcaa ctgctcaagt gtctgatcaa ggctgccggg atcgctttgg
      841 aacaggagga agtcaccctg gttgccgtcg acaaatggga caagatcggc gagcccgggg
      901 tggttcagga actgcagaat cgcggcatcg ctgccgaagc ggcttctcgc cttcttcacc
      961 tgctgaaacg accggaaggt atcagcgaaa ccgaagcgct cgatcgcctt tcaggtctgt
     1021 tgcacgacat tcccgaagcc gaaccggcga ttcaggaact gcgccaagtt ctcgcgctcg
     1081 ccacgcacac tccggccgga tctcgcctca aagtggatgc gacgcttgcc cgaggactgg
     1141 gctattacac cggcccgatc ttcgagatcc gatctccgct cgccaaggga agtctcggcg
     1201 gcggtggccg gtacgatggc cttatcggta tgttctgcaa tcgcgagatt ccggcggtgg
     1261 gattctcgat cggtttcgaa cgcgtggtcc tgcttctcga agaagccgga ctgttcccga
     1321 ccaccggagc cggagctcat atgctggtct gtcgcttcga cgacgcagat ccggtcgagg
     1381 tcatgaaagc cgcatcgata ctacgccagt caggcatcaa ggtcgagatc tatcccgaga
     1441 ccggaaagat tggaaaacag atcagctatg cggacactca gaagattccg gtcgtcggca
     1501 tcttcggtgg tgccgaaacc gccgctcagg tcatctcgct caagcatctg acatcgggcg
     1561 aacaggcgaa actgtcgtac accgaatccg tggcgaagat ccaggaatgg ctgcagaaga
     1621 cctctacagc ctgagagcag aattcgtgtt ttgacgaaac cacccccatg ccctgatcac
     1681 tccagcatgg gggtggtgat ttttccgtga tcgtctgact actttttctg cttccttgca
     1741 gcagaagtat ctccgcggcc ccagtcgtcg tcgtagcgct cgatgtcgtc ttcttcgaga
     1801 tagccgccga cctgaatctc gatgatttgc agaggaatac gaccgaagtt agacaagcgg
     1861 tgcttctgtg tcgcctgaat atagatcgag tggttctcgc gtacgaagag ttcttcattt
     1921 tcacgccgaa ccgtcgccgt acctgaaacc acaacccagt gctcgttccg atgttggtga
     1981 cgctgcaaac tcaaacgctt gcccggtttg accacgatgc gcttgatctt atagcgaggc
     2041 ccttcttcga gaatcgtgta actgccccac ggtcgatgca ccgtcagatg ctcgcgcact
     2101 tcgcggcgct gtgtctgatt cagtcgttcg acaacctcgc gtacccgctg gctctcgctc
     2161 aaagggcaga caagaagcgc gtctggcgta tcgataaccg caaggtcctt cacgccaagt
     2221 gtcgcaatcg tgcgatcccg gccgatgacc agactgttgc tggtatcgat cgtgacatgg
     2281 ttaccaacca ccacgttccc cgcgtcatct tttggtgaca cctcgaacag actgtaccag
     2341 ctgccaacat cgctccaccc aaatgtcgag ggcagcatgg cagctttgga tgtcttttcc
     2401 atcacggcat aatcgatcga aattttcggt gactttccga acgccgttcg caaagcctca
     2461 gactcactga tttgcatgcc atcaaatgct ttcatcaact caggacacgt ttttcgcaac
     2521 tcggacaaaa gagtttcaac gtgaaacacg aacatgccgc tgttccaggt gtagcgcccg
     2581 gatttcagat acccttcggc ggttttct
//
LOCUS       DAORHD010000688         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47098_length_2608_cov_4.368194, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000688 DAORHD010000000
VERSION     DAORHD010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47098_length_2608_cov_4.368194"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PKO06_14075"
     CDS             complement(<1..402)
                     /locus_tag="PKO06_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70824.1"
                     /translation="MPKAKKAPKELPPSDAAAAKLEELLAEMALDEERILANLAFDPV
                     LAGRQLKERHFTANDFQGLEANKVLLGSMLQVLEADETMNLSNLLHKMSLEKKGSHTR
                     LELAGGAERIQRLFDNPFSRAGVVMTEDLDPV"
     gene            706..1179
                     /locus_tag="PKO06_14080"
     CDS             706..1179
                     /locus_tag="PKO06_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70825.1"
                     /translation="MSIRTVLTTVIISGLTIGSAFAGTCPTKPCAMKKAKMAIVKTGD
                     ALQDKTMGAALKAKKTVTGKQTTTFVKGHYRADGSHVKGHQRKLVNPVNGPSLAAKAK
                     KQAIKAGDSVQNGVMDTGVAAKKAITGKKNKTFVKGHFKADGSHVKGHFRATAGK"
     gene            1283..>2608
                     /locus_tag="PKO06_14085"
     CDS             1283..>2608
                     /locus_tag="PKO06_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70826.1"
                     /translation="MKMPFRTSSSRSICGRQIATWSRLTRMAWLLFLSGVLCLPAPAA
                     AQPDHALLQYCPAEYHAVSIAELATQSVSPIRVSVRPVSDFITPATLAENNPLDVPLY
                     LAKRIPKPIRRLLASPSTHLILLPDLLDRAIPTLRQQGFSSGAVFLADLNTRVQVAYL
                     VFSQPRGMTVVVTHLFGQTRLEHLAWQYATFLFKAGRSLDAIEIWETQNPDTLARELC
                     FDALQDLDPDQPTVICSGYFGSLKHVLADFELFHRRHPGSLPTLAQLDHLRAIEQPGQ
                     ITTTLGGNPYFPWWQTFLTHPDGRRTRIIGFRNLYGDQTGTLFSCLLEKGFRRFVLFS
                     NGGGLAHTSLRTLYAPGVARDGQTTVPLRNLAIRRYPARALVSVPSLLVETSRWLEEH
                     RFCSLVDVEGVHAARALAPYPDSRLYYAILVSDRPGHIDITQKEEDAPDS"
BASE COUNT          508 a          823 c          727 g          550 t
ORIGIN      
        1 gaccggatcg agatcttcgg tcatgacgac gcccgcgcgc gagaaggggt tgtcaaacaa
       61 cctctgaatg cgctccgctc cgcccgccaa ctcgaggcgc gtgtgcgagc ctttcttttc
      121 gaggctcatc ttgtgcagca ggttggaaag gttcatggtt tcatcagctt cgagaacctg
      181 caacatcgaa cccagcagca ccttgttggc ttcgagcccc tgaaagtcgt tggccgtgaa
      241 gtgacgttct ttcagctgac gacccgccag aaccggatca aacgcgagat tggcgaggat
      301 ccgttcttca tcgagggcca tttccgccag cagttcttcg agtttcgcgg cagcggcatc
      361 ggagggtggc agttctttgg gggccttttt ggctttgggc acacgcattc tccctgacag
      421 gaatgagtgc catagtaccg caccgggggt gtgccgtcaa tggccgaaac cgtgatttca
      481 tcggggtgcc gagcgttcca gtgtccagca gggccggcgt cgcgccgtca ggttgatgac
      541 gatggatttt cagcgactca acggcagaaa tctggcatga tttccggctt gacacttgtc
      601 tgctgttctg ttcctcggcc tgccaggcgc agcttcgact ggtaacccag actggtacac
      661 gctttgcgta atgcctcgca tcatcaattc aagctggagg aacccatgag catccgaacc
      721 gttcttacca ccgtcatcat cagtggcctg accatcggca gcgcttttgc cggcacctgc
      781 cccaccaagc cctgtgccat gaagaaggcg aagatggcca tcgtcaagac cggcgatgct
      841 ctgcaggaca agaccatggg tgccgcactg aaggccaaaa agaccgtgac cggcaagcag
      901 accacgacgt tcgtgaaggg ccactaccgg gccgacggtt cgcatgtcaa gggtcatcag
      961 cgaaaactgg tcaacccggt gaacggcccc agcctggctg ccaaagcgaa gaaacaggcc
     1021 atcaaggccg gcgactcggt tcagaacggc gtcatggata ccggcgttgc cgcgaagaaa
     1081 gccatcaccg gcaagaagaa caagaccttc gtgaaagggc acttcaaggc tgacggttcg
     1141 cacgtgaagg gtcacttccg cgccaccgcc gggaagtaat caagcgtttc gaaacggcgc
     1201 cgccgacctt cgggtcggcg gttttgcttt cgggatgatt ggcgttgctc ttcgcgaaga
     1261 attttcgtac catgagccga ccatgaaaat gcctttcaga acctcatcga gccgatcaat
     1321 ctgcgggcga cagatcgcca cgtggtcgcg actcacccgg atggcgtggc tgctgtttct
     1381 ctccggcgtc ctctgtttgc cggcaccggc ggcggcacaa cctgatcacg ctcttctgca
     1441 gtattgtccg gctgagtatc atgccgtttc gattgcggaa ctggccaccc agtccgtttc
     1501 cccgatccgg gtgtcggtgc ggccggtttc ggatttcatc accccggcca cgctcgccga
     1561 gaacaatccg ctcgatgttc cgctctatct ggccaagcgg attcccaagc cgatacggcg
     1621 gttgttggcg agtccttcga cccatctgat tcttctcccc gatttactgg atcgcgccat
     1681 tccgactctg cgacaacagg gattctcgtc aggcgcggtc tttctggccg acctgaacac
     1741 tcgcgtccag gttgcctatc tcgtcttttc gcaaccgcgc ggcatgaccg tggttgtcac
     1801 ccatctattc gggcaaacac gactcgagca cctcgcctgg cagtatgcca cgtttctgtt
     1861 caaagcaggg cgttctcttg atgcaatcga aatctgggaa acacagaacc cggacaccct
     1921 ggcccgagaa ctgtgtttcg acgctctgca ggacctggac ccggatcagc cgaccgtgat
     1981 ctgttccggc tatttcggtt cgctcaagca tgtgctggcc gacttcgaac tctttcaccg
     2041 acggcaccct ggttccctgc cgacactcgc gcagctcgac catttgcgcg caatcgaaca
     2101 acccgggcag atcaccacga cgctcggagg caacccctat tttccatggt ggcagacgtt
     2161 tctcacacac ccggacggtc gccgaacgcg catcatcggg tttcgcaacc tgtacggaga
     2221 ccagaccggc accctgtttt cgtgcctgct cgaaaaagga tttcgccgat tcgtgctgtt
     2281 cagcaacgga ggcggcctgg ctcacacctc gctgcgcacg ctgtatgctc cgggtgtcgc
     2341 tcgcgacggc cagacgaccg tgcccttacg caatctggcg atacgacgat acccggcacg
     2401 ggcactggtc agcgttccct ccctgctggt cgaaacctct cgctggctcg aggaacacag
     2461 attctgctcc ctggtcgacg tcgagggggt gcatgccgcg cgcgccctcg caccttaccc
     2521 cgattcgcgg ttgtactatg ccattctcgt ctctgaccga cccggacata tcgacatcac
     2581 ccagaaagag gaagatgccc ccgactcg
//
LOCUS       DAORHD010000689         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47133_length_2607_cov_3.523511, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000689 DAORHD010000000
VERSION     DAORHD010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47133_length_2607_cov_3.523511"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKO06_14090"
     CDS             complement(<1..469)
                     /locus_tag="PKO06_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV70827.1"
                     /translation="MKQYRLTLRKPDGEMEVRDVHADNASAAEELARLSGAELDTLEE
                     ITPSEPWTFSLGSGVPTEELYHFTRLFATLTKAGIPLLETLLLLTEKARHPRMKQTLE
                     VIGRDIREGSGLQVAFAKHPRLFDPTYLNLLMVGEESGQLYNVLARLTDLLKKQ"
     gene            complement(512..1306)
                     /locus_tag="PKO06_14095"
     CDS             complement(512..1306)
                     /locus_tag="PKO06_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70828.1"
                     /translation="MTPNQPSKNRRLIIMLTVLSVLVAANLLVHSRRSTAPSSPRSGV
                     QATGTLAQSAATTSSSQASVLPGSVQSLPGMTASQPTDWSQALDRIGELEKRLAALPQ
                     VFLAPAPGDAFRAPSRDPLRWKAPSAPALVVVPASAPVMLPPPDKALASPPVLLGIFH
                     VQGKNRALIRDGDNVYTLSEGQSDPQLPYHLVSLTDGVRLQDAKGREIEIRDPQQEDE
                     RRLARVRDILEGRGPPTVYRISSPAALRPQSPHVPVSTANPVPPGR"
     gene            complement(1303..1866)
                     /locus_tag="PKO06_14100"
     CDS             complement(1303..1866)
                     /locus_tag="PKO06_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70829.1"
                     /translation="MTRGERATLMTFGSLAGVLLLLLGAIAWFPTAWREMGRIRNIQA
                     QLSDTRSLEAEAQQYSQQIQTRVKQVQDRLLTIERHLAVASFSLLPVSQISHTVETLA
                     DQFAETGVNIVNMSYKSRIAESGFVTLPFEILIESSYLGLRRLLHLIETHPGHLFLDR
                     LEFVSLDNERHLVQARLACLIRFQQNP"
     gene            complement(1868..2446)
                     /locus_tag="PKO06_14105"
     CDS             complement(1868..2446)
                     /locus_tag="PKO06_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70830.1"
                     /translation="MSTGFEFLPRSSRRIHADLRFPAAFILLALGLSVGAWWLLGTTR
                     HLQQEYRQKSAGLTTRKQELLQDALALIPAPDTLAAMEARINRHNVACIGPRFPWSRL
                     LSILEQALPEDSVISSILNSRTGRPQFASGDREFALTVVVADVDVANTFYSRLSAITS
                     FQNLSFTPKNEVTIQGRRGTAIEISFRFQEGD"
     gene            2419..>2607
                     /locus_tag="PKO06_14110"
     CDS             2419..>2607
                     /locus_tag="PKO06_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70831.1"
                     /translation="MEEIRNQYSCCHHPIKSFQIPGFERQVPEQRKVRALLGGGQDRC
                     PWGKLQELRGSLEGLAKSG"
BASE COUNT          553 a          683 c          845 g          526 t
ORIGIN      
        1 tctgcttctt gagcaggtcg gtcaatcgcg cgagtacgtt gtacagctga ccggattctt
       61 ccccgaccat cagaaggttc aggtaggtcg gatcgaaaag gcgtggatgt ttggcgaagg
      121 ccacctgaag tccactgcct tcgcgaatgt cccggccgat cacttcgagt gtctgcttca
      181 tgcgcgggtg tcgcgctttt tccgtgagca gcagaagggt ttcgagcagt gggatcccgg
      241 ccttggtgag cgtcgcaaac aggcgggtga aatggtacaa ctcttcggtc ggcacaccgc
      301 tgccgagtga aaacgtccag ggctccgatg gtgtgatttc ttcgagagtg tcaagttccg
      361 ccccggaaag tcgtgccagc tcttcggcgg cagaggcatt gtcagcgtgc acgtcacgta
      421 cttccatctc gccatcgggt ttgcgcagcg tcaggcgata ctgtttcatc acgtgttctc
      481 tcgagatttc atgggctgaa tcaggtgtgg ttcaacgacc gggtggcacg ggatttgccg
      541 tcgagacggg aacgtgggga gattgaggtc gaagtgctgc cggtgacgag atccgataca
      601 ccgtcggggg accacggcct tcaagaatgt cgcgcacgcg agccaggcgt cgctcgtcct
      661 cctgttgcgg atcacggatc tcaatctcac gtcccttcgc atcctgaagt cgcaccccgt
      721 cagtcaaaga taccagatga tatggaagtt gcgggtcact ctgtccttcc gacaaagtgt
      781 acacgttgtc gccatcgcga atcaacgcac gattctttcc ctgaacatgg aagataccaa
      841 gcaggacggg cggactggcg agagctttgt caggcggggg gagcatcact ggcgcggatg
      901 ccgggacgac gaccaaagcg ggcgcactgg gagctttcca gcgaagcgga tctcgtgagg
      961 gggcacggaa agcgtctcct ggagccggcg cgagaaagac ctgcggcagc gcggcgagtc
     1021 gcttttcgag ttcgccaatg cgatcgagcg cctggctcca gtcagtgggt tgcgatgcgg
     1081 tcatgccagg cagactttga actgaaccag gtaggacgct tgcctgactg gacgaagtcg
     1141 tggcagcgga ttgtgcaaga gtaccggtgg cttgaacccc ggatcgtggt gaggaaggcg
     1201 cagtggagcg acgtgagtgg accagcagat tggccgcgac gagcacgctc agaacggtga
     1261 gcatgatgat gagacgtcga ttcttgctgg gttggtttgg tgtcatggat tctgctgaaa
     1321 ccgtatcaga cacgcgaggc gagcctgaac cagatgccgt tcgttgtcga ggctgacgaa
     1381 ttcgagccga tcgagaaaga ggtgtcccgg gtgcgtttcg atgagatgca gcaggcgacg
     1441 tagacccaga tagcttgatt cgatcaagat ttcgaacggc agagtcacga accctgattc
     1501 ggcgatacgg ctcttatagc tcatgttcac gatgttgacc ccggtctcgg caaactgatc
     1561 ggccagggtc tcgacggtgt gagagatctg tgagacggga agcagagaga acgaggcgac
     1621 ggcgagatgt cgttcgattg tcagcaggcg atcttgaacc tgcttgactc gagtctgaat
     1681 ctgctgtgaa tactgctgcg cctcggcttc gagtgaccgg gtgtcgctca actgagcctg
     1741 aatgtttcgg atgcgcccca tctcgcgcca ggcggtagga aaccaggcga tcgcaccaag
     1801 gagaagcaag agtactccgg ccagggagcc gaaggtcatg agggtggctc gttctccgcg
     1861 agtcatctca atcaccctcc tgaaagcgga acgagatctc gatagctgtg ccgcgccggc
     1921 cttgaatcgt cacctcgttc ttcggtgtga aactcagatt ctgaaaagac gtgattgccg
     1981 acagccggct gtagaaagtg ttggccacat ccacatcggc gacgacgaca gtcagggcaa
     2041 actcccgatc ccctgacgcg aactgcggtc ggccggttcg ggagttgaga atcgaggaaa
     2101 tcacggaatc ctctggtaat gcctgttcaa ggatggataa cagccgactc cagggaaatc
     2161 gcggcccgat gcaggcgacg ttgtgccgat tgatacgtgc ttccatcgca gccagagtat
     2221 ccggggcggg aatgagcgca agagcgtctt gcagaagttc ctgtttgcgg gtcgtaagac
     2281 ccgcggattt ctggcggtat tcctgctgca ggtgtcgggt ggtgcccagc agccaccagg
     2341 cgccgacgga aagcccgaga gcgaggagga taaacgcggc gggaaatcgc agatcggcgt
     2401 gaatgcgtct ggatgagcgt ggaagaaatt cgaaaccagt actcatgctg ccatcacccg
     2461 atcaaatcgt tccagattcc agggttcgag cgccaggttc ccgaacagag gaaagtaaga
     2521 gctctgctcg gcggcggcca ggatcgttgc ccctggggca agcttcagga gctgcgcggc
     2581 tcgctggaag gtctggcgaa atcaggc
//
LOCUS       DAORHD010000690         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47134_length_2607_cov_3.367163, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000690 DAORHD010000000
VERSION     DAORHD010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47134_length_2607_cov_3.367163"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="PKO06_14115"
     CDS             <1..1340
                     /locus_tag="PKO06_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNV70832.1"
                     /translation="IQADALRTLGQRDRALALLESLTRVSGLDEGTRALAAQIRGLIL
                     VSVNRHDAAEADFRTALAWGKPPVDVDLVRVRLIRAAYSAGHDRVAFENLQKLCQTRV
                     TVPVLPGLVYELALKRYDDGYPQAAVPYFLLLPEYFPGHYRADDALGYAAVSLGTTTA
                     DGKKLLARLKQRYPHSFFLYWLDESSRASGLPSGGAAKATVPNWAAKRLPAWKILLKS
                     DFAGWARDEIRQMQDEHPLEPGLFQVLMQIYEQAGDYNQVTACGERYLKNLLDAGKSA
                     GDMPRWAWEGYYPRPFWAKVQAEAKKNGLDPFWVLSIMREESHFNPQTLSRSNAMGLM
                     QILPSTGKWIAGKLGIKGKFNKDSLWDVARNISFGSWYLAYLRDLFNGDLFLAAAAYN
                     GGQGNIQRKVEAGPHSGLPVLSRLDRVPLPETRDYYKKVMGSWWNYRRLYGKN"
     gene            complement(1516..1734)
                     /locus_tag="PKO06_14120"
     CDS             complement(1516..1734)
                     /locus_tag="PKO06_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV70833.1"
                     /translation="MTIPELMKFLQVKRNTIYTLRRLGMPTVKIGRSVRFRLDSIMTW
                     LSHHEKSQMSDLERPLVGTVIPDSSHLL"
     gene            complement(2092..>2607)
                     /locus_tag="PKO06_14125"
     CDS             complement(2092..>2607)
                     /locus_tag="PKO06_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70834.1"
                     /translation="SLAWGYFDHPPGVAFLGLGTWLAPGSALAGRLGTLVAATLSFLV
                     LASLVIHNMVHSTRNVVLNYWTWACNDHQAKIQAAHHRAVQTLPPGAPQHQTLDLAYL
                     RFRGDLKEDLVCPASGTYLLNIASPTPVVFCSFHGSYVHGIIGKRNPHESFVWWLRYV
                     LGLYTKRQKII"
BASE COUNT          588 a          660 c          787 g          572 t
ORIGIN      
        1 tgattcaggc agatgcgctg cgaacgctcg gtcagcgaga ccgagctctg gcactgctcg
       61 aaagtctgac ccgagtttcc gggctcgatg agggaacacg agctctggcg gctcagattc
      121 gagggttaat tctggtgtcg gtgaatcgcc acgatgccgc cgaggccgat tttcgcacgg
      181 ctctggcgtg gggaaaaccc ccggtcgatg ttgatctggt tcgtgtgcgg ctgattcggg
      241 ccgcttattc agccggacac gatcgcgtgg cgttcgaaaa tctgcagaaa ctctgtcaga
      301 cgcgggtgac cgttcccgtg ttgcctgggt tggtctatga attggctctc aaacgttacg
      361 atgacggata tccgcaggcg gccgttccgt attttctgct gttgccggag tattttcccg
      421 ggcactatcg agccgacgac gcgctggggt atgcagccgt ttcactgggg acaaccactg
      481 cggatggcaa gaagttgctg gctcgtttga aacagcgata cccgcactcc tttttcctct
      541 actggctcga tgagagttcc cgcgcgtctg gtctgccgtc aggcggtgcg gcaaaggcga
      601 cggtgccaaa ctgggcagcg aagcgtttgc cggcctggaa gattcttctg aagagtgatt
      661 tcgccggctg ggcgcgcgat gagattcgcc agatgcagga tgaacaccca ctcgagccgg
      721 gtctctttca agtgttgatg cagatctacg aacaagcggg tgattacaat caggtgacgg
      781 cctgcggaga gcggtatctg aaaaaccttc tcgatgccgg caagtcggcg ggtgacatgc
      841 cgcgctgggc ctgggagggc tactatccgc gcccgttctg ggccaaagta caggctgaag
      901 cgaaaaagaa cggcctcgac ccgttctggg tgctttcgat catgcgcgaa gagagccatt
      961 tcaacccaca gacgctgtca cgcagcaatg ccatgggttt gatgcagatt ctgccttcga
     1021 cgggcaagtg gattgccgga aaactcggca tcaaggggaa gttcaacaag gacagtctgt
     1081 gggatgtggc gcggaacatc tcatttggca gctggtacct ggcgtatctg cgcgacctgt
     1141 tcaacggcga tctgtttctc gcggccgcag cttacaatgg cgggcaggga aacattcagc
     1201 gaaaagtcga agccggccct cactccgggt tgccggtgct ttcaagactt gatcgggtac
     1261 cgttgcccga gactcgcgac tattacaaaa aggtcatggg cagctggtgg aactaccgtc
     1321 ggctgtacgg aaaaaactga acggagttca cccaccgatg cggcgttgcc gtctgcggcc
     1381 acgccggtcg gtaggttctt cggccatcgt ttcgagcagt cgaaagatgg tttggcagaa
     1441 caacatacct gccgtggctg acacggcgag gaaaccgaag atttcgggaa gaggggcggt
     1501 catctgccgg ccgattcaga gcagatgtga actgtcagga atgacggtgc cgacgagagg
     1561 gcgttcgaga tctgacatct ggcttttttc gtggtgactc agccaggtca tgatcgaatc
     1621 caggcgaaag cgcacggatc gaccgatttt cacggtcggc attcccagcc gccgcagcgt
     1681 atagatggtg ttccgtttga cctgaagaaa cttcatcaat tcggggatgg tcatgagggt
     1741 atctgctttc acaaaggcct cccgatgctc gtagttcgtc gtgattgggt taatgtgact
     1801 atcgtcatcg gcgaaagcga acttgagccc ccaaaaaaag gaaaaaagaa tctaaagtct
     1861 tcggtgaatc gttccgataa tacctgtgga gttcagaaac gaaatgttca gcgatgaaca
     1921 actcgcgact ggcctctgac aaccaactcc ctaactccct ttcaatgtgt cgtgaaaacg
     1981 gcacattttt tatttttccg aagaacgaat ctacacgaaa aatgcctgca tctggataac
     2041 agatgagagt atattatctt tttcaaagac tctgttaaag actattttga gtcagatgat
     2101 cttctgtctt ttcgtgtata acccgagcac gtagcgcagc caccacacga aagattcgtg
     2161 tggattgcgc ttaccgatta ttccgtgaac atatgagcca tgaaatgaac agaacacaac
     2221 gggtgtgggt gacgcgatgt tgagaagata ggttcctgaa gccggacaga ccagatcttc
     2281 cttcagatct ccgcgaaacc gcagataggc gagatcgagt gtctggtgtt gtggagcccc
     2341 aggaggaagc gtctgtacgg cgcgatggtg tgctgcctgg attttcgcct gatgatcgtt
     2401 acaggcccag gtccagtagt tgagaacaac gtttctggtc gaatgcacca tattgtgaat
     2461 gaccagggag gcgagaacga ggaagctgag ggtggccgcg accagggtcc cgaggcgccc
     2521 cgccagggcg gaacccgggg ccaaccaggt gccgagcccg agaaaggcga cacctggcgg
     2581 gtggtcgaag tagccccagg cgaggct
//
LOCUS       DAORHD010000691         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47163_length_2606_cov_3.374755, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000691 DAORHD010000000
VERSION     DAORHD010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47163_length_2606_cov_3.374755"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKO06_14130"
     CDS             <1..511
                     /locus_tag="PKO06_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009634100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV70835.1"
                     /translation="ICVVSGGARGIDAAAHRACLEQGGQTIAVIATGIDIAYPPEHAE
                     LFREISRTGCVLSEFFPGTPPVASHFPTRNRIIAGLAQAVVFIEGNARSGGLITVRKS
                     LQAKRPVWAARHPDLLDGARPPLERLLSPGRFFSSPGELFELLAPLFPLGRHPPPRSE
                     PDRVPREEA"
     gene            511..1035
                     /locus_tag="PKO06_14135"
     CDS             511..1035
                     /locus_tag="PKO06_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70836.1"
                     /translation="MRRFLLLLLCVLIAFLLLSLGYLEYRVDAIIRAPASLDLSRKTG
                     STALRLINSASDMLRVTGFEPSASATLPLNAPARLSFRYGLTSSRKALIYVRPTYQGI
                     RVSGFRSSGSPVYETGVGEGTFEMSSAQPARCDALELFMEVGDLPLMVYAQLVHGTDL
                     WLLKLRIPVFWEWK"
     gene            1158..1787
                     /gene="lexA"
                     /locus_tag="PKO06_14140"
     CDS             1158..1787
                     /gene="lexA"
                     /locus_tag="PKO06_14140"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNV70837.1"
                     /translation="MTPDPLTPRQKEVLDFLQRFIDEHGYAPSMAEVCTGMQLTSTAT
                     AHKLVQTLREKGYLDAQPRRSRGLTMKTRPLELTGLPSVDVPLLGRVAAGQPIEHNAT
                     PGVISIPEWMLGRGETYVLEVKGDSMIDEAIKDGDLVIVEKRDHAENGEMVIACIDNE
                     TVTMKRLYREHRRIRLQPSNPAMAPIIVENRHVTVSGVVIGVLRKYRRW"
     gene            complement(1791..>2606)
                     /locus_tag="PKO06_14145"
     CDS             complement(1791..>2606)
                     /locus_tag="PKO06_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70838.1"
                     /translation="DPEVARLALEVINRRLPDAGTELAIELFPHSSATEKRSLLWNLK
                     QKHSALPAASVGILRQAARDSDRGIRDDALEILCDQAYHSLEAWKALNDVKGTITDPA
                     WRERIGRQLADSVQKSNPDMARQFVFAAIRGASADDRRAAAHKVFRLGLPADALLPEV
                     AALVTTYGSDGKFFEALLRGFTEQGMSRLKNVEATELEQILNKAVNHPDKMVKYFGYS
                     LMYSAWSKGAKKFAEPLKFGLTRESDVWLKGQIKNKVDAIERSENPESAAEGD"
BASE COUNT          473 a          849 c          734 g          550 t
ORIGIN      
        1 gatctgcgtg gtcagcggcg gagcacgcgg gatcgatgcg gcagcgcacc gggcctgtct
       61 cgaacagggc gggcagacga tcgccgtcat cgccaccggc atcgatatcg cttacccgcc
      121 cgaacacgcg gaactgttca gagagatcag tcgaacggga tgcgttttgt ccgagttctt
      181 tccgggcaca cctcccgtcg ccagccactt tccgacccgt aatcgcatca tcgccgggtt
      241 ggcgcaggcc gtcgtcttca tcgaagggaa cgcccgcagc ggcggcctga tcacggtgcg
      301 caagagcctg caggccaaac gccccgtctg ggccgccaga cacccggacc tgctcgacgg
      361 cgcacggccg cctctcgaac ggttgctgtc cccaggccgg ttcttttcct cacctggtga
      421 gcttttcgaa ctcctggccc cgttgttccc cctggggcgg caccctccgc cacgttcgga
      481 accagaccgc gtcccacgag aggaagcctg atgcgacgct tccttctgct gctcctgtgt
      541 gtgttgatcg cttttctgct gctgtcgctc ggatacctcg agtatcgggt cgatgcgatc
      601 atacgcgctc ccgcatcgct cgacctcagc aggaaaaccg gctcgacggc tctccgcctg
      661 atcaactcag ccagcgacat gctcagagtg acaggttttg agccctccgc ctcggcgacc
      721 ctgcctctca acgcaccggc acgtctgtcg tttcgttatg gtctcacctc gtcccgcaag
      781 gctctgatct acgtgaggcc cacgtatcaa gggatccgcg tctcagggtt cagaagcagt
      841 ggctccccag tctatgaaac cggtgtcggc gaaggcacgt ttgagatgtc ctcagcgcaa
      901 ccagctcgct gtgatgcgct cgaactgttc atggaggtcg gtgatctccc cttgatggtg
      961 tatgctcagc tggtgcacgg aacggacctc tggctgctga agctccgcat ccctgttttc
     1021 tgggagtgga agtaggactc tttcgacggc gcctgctttc ttcccttccg gattttcgcc
     1081 ttcccttcgc ttgacattcg ccttttgttc gcttaggctg aagacagcca tcaacttttt
     1141 gcgagcagag gtgtttcatg acccccgatc ctttgactcc ccgccagaaa gaggttctcg
     1201 actttcttca acgtttcatc gatgagcacg gctacgcacc gagcatggcc gaggtctgca
     1261 ccggcatgca gctcacgtca acggcgactg cccataagct ggtacagacc ctgcgcgaga
     1321 aagggtatct cgacgcacag ccgcggcgct ctcgcggcct gacgatgaaa acccgccccc
     1381 tcgaactcac gggcctgcct tcggtcgatg tgccgctgct cgggcgggtc gccgccggtc
     1441 aaccgatcga acacaacgcg acaccggggg tcatctccat cccggaatgg atgctcggcc
     1501 gcggcgaaac ctacgtgctc gaggtcaagg gcgactcgat gatcgacgaa gccatcaaag
     1561 acggcgatct cgtcatcgtc gagaaacgtg atcacgcgga gaacggcgag atggtcatcg
     1621 cctgcatcga taacgaaacc gtgaccatga agcgattgta tcgcgaacac cgccgcatcc
     1681 gcctgcagcc ttcgaatccg gcgatggccc ccatcatcgt cgaaaaccgt cacgtcacgg
     1741 tcagcggtgt cgtcatcggt gtactgcgaa agtaccggcg gtggtgaccg tcaatcccct
     1801 tctgctgctg actccgggtt ttctgaacgc tcaatcgcgt caaccttgtt cttgatctga
     1861 cctttcaacc agacatcaga ctctcgggtc agtccgaatt tgagcggttc ggcgaacttc
     1921 ttggcgccct tgctccaggc tgagtacatc agcgaatagc cgaagtattt gaccattttg
     1981 tccgggtgat tgaccgcctt gttcaagatc tgttcaagct ctgtcgcctc aacattcttc
     2041 aacctcgaca tcccctgctc ggtgaagccg cgcagcagcg cctcgaagaa tttgccgtcc
     2101 gagccatagg tggtcaccag agccgcaact tcaggcagca gggcatcggc cggcagacca
     2161 aggcgaaaaa ccttgtgcgc ggcggcccgc cgatcatcgg cactggcgcc gcgaattgcg
     2221 gcaaacacga actgcctggc catgtcgggg ttgctcttct gcacgctgtc ggcgagttgt
     2281 ctgccgatgc gctcccgcca ggccgggtcg gtgatggttc ccttcacgtc gttcagcgct
     2341 ttccaggctt ccaggctgtg atacgcctga tcgcacagaa tttcgagggc atcatcgcga
     2401 attccacggt ctgaatctcg cgccgcctga cgcaggatgc cgaccgaagc cgccggcaac
     2461 gccgaatgtt tctgcttgag attccagagc aggctgcgtt tttccgtcgc gctggaatgg
     2521 ggaaacagtt ctatcgccaa ctccgttccg gcatccggca gccgccggtt gatcacctcg
     2581 agagccagcc gggcaacttc gggatc
//
LOCUS       DAORHD010000692         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47303_length_2601_cov_3.947368, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000692 DAORHD010000000
VERSION     DAORHD010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47303_length_2601_cov_3.947368"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1132)
                     /locus_tag="PKO06_14150"
     CDS             complement(167..1132)
                     /locus_tag="PKO06_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70839.1"
                     /translation="MTAPVPHQHTWRVEGLDCPNCARQLESDIREQTGVQHVELDFMG
                     GMLTVLCSIENDCEKTLAEIAAQHGASFHAPIPSATASTDTESRPWPAEAWYMLCSGV
                     PLLIGWVTGSIAWYLASLAIAGSPVLGKTLQELRLRTLAMNSLMLIAAIGAVAINEHQ
                     EGAMVLFLYTIARWLERLNSEKARRSIELLKTKIPALAHVIDDHGDFDLPVARVEPGT
                     LIRIKPGERIPLDGKVVQGSSTCDEQILTGESLRVTKAPGTTVYAGTFNGEGALEVET
                     TQPVAQSRFSRIIDSVERIQGQKTRFQTTIERFAEIGASIKKITG"
     gene            complement(1143..1490)
                     /locus_tag="PKO06_14155"
     CDS             complement(1143..1490)
                     /locus_tag="PKO06_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV70840.1"
                     /translation="MNKCSYIHANNIPDGEQLATVAELFKCMGDPCRLAMLSLLHAHE
                     LPVGEIAQRLQHTPSAVSHQLRLLRSLRLVRTRRDGRRILYALDDQHVAELLEVAFVH
                     SRHPHVDDRDNEK"
     gene            complement(1679..>2601)
                     /locus_tag="PKO06_14160"
     CDS             complement(1679..>2601)
                     /locus_tag="PKO06_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70841.1"
                     /translation="VTTDVLALPDITALVASQAAFVRNWGGFTPGALVAYLGRSNDLD
                     NDGREDLRVIPSIDGTRVSFATVISSDTSLYNPTSYAQSPTLASYTDVTLLEDFRTGN
                     TRTDRNFTREAVNFLIGLRFKRGATQDTYLKLLVRRTDMADGRFDGIAAEYQFVTTTG
                     TAVSSGTKTFLMAGASPIVGAVTATDFLTDYPTASPTLLSYLDTTNGFGGSNMTVPLL
                     RAENAKLDLTDIRQADEYTDTYYRTTSALALKDELGAARALQVGDLFTAYFPKTRHYA
                     MFKITNINTVLQTITVDYYVNSTADELRFF"
BASE COUNT          539 a          674 c          812 g          576 t
ORIGIN      
        1 cgcgtgctcc gaacgacggc cctccgatat atacggaatc ggccccgtgg ttgatcgctt
       61 cgatgccgaa atcggcgttc ttcgcagggg caaggagctc caatgtgtgg tcgtgttcag
      121 tcatcgggga gtgagttgtt gcgggcctgt attgtaactc gttgaattat ccagtgattt
      181 ttttgatgga tgcaccgatt tcggcgaatc gttcaatggt tgtctgaaac cgggttttct
      241 ggccctgtat gcgttcgacg gaatcgatga tacgtgaaaa gcgtgactgg gccaccggct
      301 gggtggtttc gacctcgaga gccccttccc cattgaatgt tccggcatag acggtggtac
      361 caggggcctt ggtgacccgc agggactcgc cggtcaagat ctgttcatcg catgtcgacg
      421 atccctgtac gacctttcca tcaaggggaa tacgctcacc gggtttgatg cggatcagcg
      481 tgccgggttc gacacgagcg acgggaagat cgaagtcacc atgatcgtcg atgacatgag
      541 ccagcgcagg aatcttggtt ttgagcagct cgatcgagcg acgtgccttt tcgctgttga
      601 ggcgctcgag ccatcgtgcg atggtataca ggaacagcac catggcacct tcctgatgct
      661 cgttgatggc gaccgcgcca atcgcagcga tcagcatcaa tgagttcatg gccagggtgc
      721 gcaggcgcag ttcctgcaag gtttttccca gcaccgggct gccggcgatc gccagtgacg
      781 cgagatacca ggcgatactg ccggtgaccc agccgatcag caacggcacc ccggaacaca
      841 gcatgtacca agcttcggcg ggccaggggc gagattcggt atcggttgat gccgtggctg
      901 acgggattgg cgcatgaaag ctggctccgt gctgggccgc gatctcggct aacgtctttt
      961 cgcagtcgtt ctcgatcgaa caaagaaccg tcaacatgcc acccatgaaa tcgagctcta
     1021 catgttgaac accggtttgt tcacgaatgt cactctcgag ctgtcgcgcg cagttcggac
     1081 agtcgagccc ttcgacgcgc caggtgtgct gatgtggaac gggggctgtc atgggagtta
     1141 cctcactttt cgttgtcccg gtcgtccaca tgagggtggc gtgagtggac aaaggcgacc
     1201 tcgagcagtt ccgcgacgtg ttggtcgtcg agcgcataga gaatccggcg accgtcgcgc
     1261 cgggttctga ccagacgcag actgcggagc agacgcaact gatgtgaaac cgctgatggc
     1321 gtgtgctgca aacgctgcgc gatctcgcca accggcaact cgtgcgcatg cagcagagac
     1381 agcatcgcga gccggcacgg atcgcccatg cacttgaaca actcggcaac cgtcgcgagt
     1441 tgctctccat cgggaatatt gtttgcgtga atatatgaac acttgttcat atattaaata
     1501 tatcgttttt tcctcagtgg tgcaagcggt tcctgactgt caatggtcga taggcgagga
     1561 aaacagcttg tgatcaactc aagccggcag attcggatgt cggtcgtttt gtgtcgcagt
     1621 cagaactcgc tgtcattccc cagagccgag atgggttgcc aatgactgag gccgcggatc
     1681 agaaaaaccg gagttcgtcc gcggtgctgt tcacgtagta atcgacggta atggtctgca
     1741 gcactgtatt gatgttggtg attttaaaca tggcgtaatg gcgggttttg ggaaagtaag
     1801 cggtgaagag atcgccgacc tgcagcgcgc gtgccgcccc gagttcgtct ttgagcgcca
     1861 gagcagacgt cgtgcggtag taggtatcgg tgtattcatc ggcctgtcgg atgtcggtta
     1921 gatcgagttt cgcgttttcg gcgcgaagca gcggaaccgt catattgctg ccgccgaagc
     1981 cgttggtcgt gtcgagataa ctcagcagtg tcggtgaagc ggttgggtag tcagtcagga
     2041 aatccgtcgc ggtgacggct ccgacaatcg gagaagcgcc ggccatgaga aacgtcttgg
     2101 tgccgctcga gaccgctgtg cccgtggttg tgacgaactg gtactccgcg gcgataccat
     2161 cgaagcgacc gtcggccata tcggtacggc gcaccagtaa cttcagatag gtatcctgag
     2221 ttgctccgcg cttgaagcgc aggccgatga gaaaattgac cgcttcgcgc gtgaagtttc
     2281 ggtcagtgcg cgtgtttccc gtgcgaaaat cttcgaggag ggtgacgtcg gtataggatg
     2341 cgagtgtcgg tgactgcgca tatgaggtcg gattgtacag ggacgtatca ctggaaatga
     2401 cggtggcaaa ggaaacgcgt gtgccatcga tcgaaggaat cacgcgcagg tcttcacgtc
     2461 cgtcgttatc gagatcattt gatcgaccga gatacgcgac cagtgcgcca ggagtgaatc
     2521 cgccccagtt gcgaacgaag gcagcctgcg aggcaaccag agccgtaatg tccggcaacg
     2581 ccagaacgtc ggtggtgacc g
//
LOCUS       DAORHD010000693         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47396_length_2598_cov_3.202123, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000693 DAORHD010000000
VERSION     DAORHD010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47396_length_2598_cov_3.202123"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PKO06_14165"
     CDS             <1..1669
                     /locus_tag="PKO06_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70842.1"
                     /translation="PAEKDEKIKIRARLLNQYAPGFLPTGTFFKLTPPTLEIRVGTFN
                     DVYLLTDLTLSLSSKITLTLPLQLNIPAMSQSKITGVTVSADIPGLWKDPLGLKGLSI
                     GNLSLAGKFCSMPTLALSGILDLGKGYKFDLTGALSFTSPVALSALRCKLSRVLSLGD
                     LIEIQGILMKNVFPKMPLPSLSAISLPIDELKVKDPEFCIAEVDVPPLGIQKGITVKG
                     TLLFGAVELGGVDFWMLADKGVRCKAWVKRFKIGDLTVSGAGADKKDFTADDSPIIDL
                     EYWPATQPPYTLKQHCYLSGRADLLGAHLKLFLDIAKDRLRLDMTGDFGDLLQIQVKG
                     QGEHSLLTDITKGGSLSCSGKVQTAFIETLERAIIQSVSSNAAVSAAVKVVGSCLLTI
                     RSISVSGSLDQFAKGSLPSCSAEFAAFGQGFTISLPNIPNTGLANTLKTRISEKITSL
                     AAELVKDPIAFVGKVFSNVVSLGEDVGRELVKVFGQSPAETWKDAEKQAEKQAEKARQ
                     AAETSIKVVSQTATDLANAAASTAKKVADTMTDIATGAWDTISGWFS"
     gene            complement(1708..>2598)
                     /locus_tag="PKO06_14170"
     CDS             complement(1708..>2598)
                     /locus_tag="PKO06_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70843.1"
                     /translation="TLPESWHGKARLFWRSGLPDHARLCQEKVRALTFGEIPRELWLS
                     GRLEILSPTSETPVFLDEQAAGKARVSLAEVKPGEHRLRWESGPSRRFFLEEGECLRM
                     HWAGRNDEISIQSFVPEVTEWKVNLNPDSTSVSPADSLLKPYMVRRPTEWPVDSDCAS
                     ILADVPGPISPFGTEGRLTIETVFRLLLEEILADGVLDHAEHDLIRAVRYRLLIDSHS
                     FRAIMDEARKKVLASRRVEKSGHLDHKSLYARVYRKACEDGVVDPSEKILLGVIAECL
                     FLTKEDVERIEREAEKADRI"
BASE COUNT          606 a          761 c          702 g          529 t
ORIGIN      
        1 tccggctgag aaagatgaga agatcaagat cagagctcga ctactgaatc agtatgcccc
       61 tggcttcctg ccaacaggaa cattcttcaa gctgactccg ccgacactcg agattcgtgt
      121 cggcacattc aacgatgtgt atctgctcac cgacctcacg ctgtcgttat ccagcaagat
      181 caccctgact ctgccgttgc agctgaacat tccggccatg tcccagtcca aaatcacggg
      241 agtgacggtc agtgctgaca ttccgggtct ctggaaagac ccgctgggcc tcaagggact
      301 ttcaatcggc aacctcagtc tcgccggcaa attctgctcc atgcccactc tcgccctttc
      361 gggaattctc gatctcggca aagggtacaa gttcgatctg accggcgcgc tctcgttcac
      421 ctctccggtg gctctctcag cgctgcgctg caagttgagt cgcgtcttga gtcttggcga
      481 tctgatcgag attcagggaa ttctgatgaa gaatgtgttc ccgaagatgc cgttgccgag
      541 cctctcggca atttccttgc ccatcgacga actgaaggtg aaagatccgg agttctgcat
      601 cgccgaggtg gatgtaccac cgctgggaat tcagaaggga atcacggtaa agggaacgct
      661 gctgttcggc gccgtggagt taggcggcgt tgatttctgg atgctggctg acaagggagt
      721 cagatgcaaa gcgtgggtga aacgattcaa gatcggtgat ctgacggttt cgggcgcggg
      781 cgcagacaag aaagatttca ccgcggatga cagccccatc atcgatctcg agtattggcc
      841 cgccacacag ccgccctaca ccctcaagca gcactgctat ctcagcggtc gagccgatct
      901 gctcggcgct cacctgaagt tgtttctcga catcgccaaa gacagactgc gactcgatat
      961 gaccggagac ttcggtgatc tgctgcagat tcaggtcaaa ggtcaaggcg agcactcact
     1021 cctcacggac atcacaaagg gcggcagtct gtcctgttcg ggcaaggttc agaccgcctt
     1081 catcgagacg ctcgaaaggg ccatcatcca gagcgtctca agcaacgcag ctgtcagtgc
     1141 ggcggtgaag gtcgtcggca gctgcctgct gaccatcaga agcatcagcg tcagcggttc
     1201 tctcgaccag ttcgccaaag ggagtctccc ctcctgctca gcagagttcg ccgcgttcgg
     1261 gcaagggttc accatctctc ttccgaatat tcccaatacc ggtctggcaa acacgctgaa
     1321 gaccaggatc agcgaaaaga tcaccagcct ggcggccgaa ctggtgaaag atccgatcgc
     1381 ctttgtcggg aaggtcttca gcaatgtcgt atcgctgggc gaagacgtcg gcagggaact
     1441 ggtcaaggtc tttggtcagt cgccggcaga aacctggaaa gatgccgaaa agcaggccga
     1501 aaaacaagcc gagaaagcac ggcaagccgc cgaaaccagc atcaaagtgg tatctcagac
     1561 ggcgacagat ctcgccaacg ccgccgccag caccgccaag aaagttgccg ataccatgac
     1621 cgacatcgcc accggagcct gggatacgat ctcaggctgg ttcagctgac acgcatcggt
     1681 tcttacgcgc ggtcaaaggc cgatcgatca gattcgatcg gccttttccg cttctcgttc
     1741 gatacgttca acatcttctt tcgtcagaaa caggcattca gcgattactc cgagcagaat
     1801 cttctcggac ggatcaacga caccatcttc gcaggccttg cgatagacgc gcgcatacag
     1861 cgatttgtga tcaaggtgcc cggacttttc aacgcgacgc gaggccagca cttttttccg
     1921 cgcttcatcc atgatggctc tgaagctgtg tgaatcgatc agcagacggt agcgaacggc
     1981 gcggatcagg tcatgctctg catgatcgag cactccgtca gcgagaatct cttcgagcag
     2041 cagacgaaaa accgtttcga tggtcagtcg tccctcggtt ccgaacggcg aaatgggacc
     2101 aggcacatcg gccagaatgg aggcacaatc actgtcgacc ggccattccg tcggtcggcg
     2161 caccatgtaa ggtttcagaa gggagtcggc aggcgagaca ctggtgctgt cgggattgag
     2221 attgaccttc cactccgtga cttccggaac aaatgactga atcgagattt catcattccg
     2281 acccgcccag tgcatgcgca aacattcgcc ttcttcgaga aaaaaccgac gcgatggtcc
     2341 ggattcccag cgcaaacggt gttctcccgg cttcacttcg gccagcgaaa cccgcgcctt
     2401 gccggctgcc tgttcatcaa gaaaaaccgg tgtctctgac gtcggtgaca gaatctcgag
     2461 acgtcccgac aaccagagct cacgcggaat ttcaccaaag gttaacgcgc gcactttctc
     2521 ctgacacaaa cgcgcgtgat caggcaaacc ggaacgccag aataaacgag cctttccatg
     2581 ccaggactcg ggcagcgt
//
LOCUS       DAORHD010000694         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47418_length_2597_cov_3.921322, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000694 DAORHD010000000
VERSION     DAORHD010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47418_length_2597_cov_3.921322"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKO06_14175"
     CDS             complement(<1..509)
                     /locus_tag="PKO06_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70844.1"
                     /translation="MNVPSRSLPAALNPLDDWVLYGLSLPDPVDRRLALELASDFTDE
                     TILESVRLISRSDPDPYCRTAARAVIQRQVLLQRARDTIKEEIDLSPASLAPLCERED
                     PLLRPALLQVIRRRPSADTLERWRAFLSGPTTSPLAAVGLALLARYGAAHDAALAPRS
                     LESTDTETV"
     gene            complement(661..2124)
                     /gene="ahcY"
                     /locus_tag="PKO06_14180"
     CDS             complement(661..2124)
                     /gene="ahcY"
                     /locus_tag="PKO06_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNV70845.1"
                     /translation="MPKSDKHATALEQETKTTVNTKRLPYKVADLSLAELGRKEIEVA
                     EKEMPGLMALREKYGQIKPLKGAKIMGSLHMTVQTAVLIETLKELGADVRWCSCNIFS
                     TVDSAAAAVAKAGTPVYAWKGETLEEYWWCTNEALTWPDGSGPTLIVDDGGDATLMIH
                     LGYEAEKDATVLNYHAHGEDEQQLLALLKRLLKDEPKKWTNMVPHIKGVSEETTTGVH
                     RLYQRHEAKKLLFPAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVCVVCG
                     YGDVGKGSAQSLKNFGARVTVTEIDPICALQAVMAGYSVLTVEDMLKTTDIWVTTTGN
                     CEIITAEHMSKMKDQAIVCNIGHFDNEIQMAELEKWPGIKKTNIKPQVDKYTFKDGHS
                     VYVLAEGRLVNLGCAHGHPSFVMSTSFTNQVLAQLDLWTNKRPVGVTTLSKKLDEEVA
                     RLHLEKIGVKLTKLTKKQADYIGVPIEGPYKNDQYRY"
     gene            2265..>2597
                     /locus_tag="PKO06_14185"
     CDS             2265..>2597
                     /locus_tag="PKO06_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70846.1"
                     /translation="MSWYLWGCSALTLGAFWLIWYYAWRNTSGHTGIPLAPTTWLFLF
                     MAVTGLLTRTALGKGLDARRPARLLILCGGLGSAFPTFLAQFQLLSPYETWLKNGQPL
                     VHARRDELL"
BASE COUNT          492 a          767 c          769 g          569 t
ORIGIN      
        1 ttcacggtct cggtatcggt cgactcaaga gagcgggggg ccagagcggc atcgtgcgcc
       61 gcgccgtatc gcgccaggag agccaacccg acggccgcga gcggagaagt ggtcggtccg
      121 gaaagaaacg cacgccaccg ctccagcgtg tccgcactgg ggcgacgtcg gatcacctgc
      181 agcagtgccg gtcgcagcag cgggtcttca cgctcacaca acggagcgag cgacgcgggt
      241 gagaggtcga tttcctcttt gatcgtgtcg cgggcacgct gcaacaggac ttgtcgctgt
      301 atgaccgctc gcgccgcagt ccggcagtag ggatcagggt ctgagcgact gatcaggcgc
      361 accgactcga ggatcgtctc atccgtgaaa tccgaagcga gctcgagggc cagtcgccga
      421 tcgaccggat cgggaagcga cagaccgtag agaacccagt catcgagagg gttcaacgcg
      481 gccggaagcg atcgcgacgg aacgttcatc gcctgttcca cggctcagag cgctgatgac
      541 ctgacccaac cgccagcctg ttccccatga ttcgagtata tcatggccgc cagaccggct
      601 aaagaggctt ggggcgcccg gtcagaccgg gcgccccaag tgagatgcga aacgcagacc
      661 tcagtagcgg tactggtcgt tcttgtacgg cccttcgatc ggaacgccga tgtagtcagc
      721 ctgtttcttg gtgagtttgg tcagcttcac gccgatcttt tcgagatgca ggcgggcgac
      781 ttcctcatcg agctttttcg acagggtggt cacgccaacc ggacgcttgt tggtccacag
      841 atcgagctga gccagaacct ggttggtgaa tgaggtgctc atgacaaagc tcgggtgccc
      901 atgcgcgcaa ccgagattga ccaggcggcc ttcggccaga acgtagacac tgtgcccgtc
      961 tttgaaggtg tatttgtcca cctggggttt gatgttggtc ttcttgatac cgggccactt
     1021 ttcgagctcg gccatctgaa tctcgttatc gaagtgaccg atgttgcaga cgatcgcctg
     1081 gtccttcatc ttcgacatgt gctccgcggt gatgatctcg cagttgccgg tggtggtcac
     1141 ccagatatcg gtggtcttca gcatgtcttc gacggtcaac accgagtaac cggccatgac
     1201 ggcctgcaaa gcgcagatcg gatcgatctc ggtcaccgtg acgcgggccc cgaagttctt
     1261 cagactctgg gcggagcctt tgccgacatc gccgtacccg cagaccacgc aaaccttgcc
     1321 ggcgaccatg acatcggtgg cgcgcttgat gccatcgacc agactttcgc ggcaaccgta
     1381 caagttgtca aactttgatt tagtgacaga atcattgacg ttgatggccg ggaacagaag
     1441 ctttttggct tcgtgccgct gatagaggcg atgcactccg gtcgtcgtct cttcgctgac
     1501 acccttgata tgcggaacca tgttcgtcca cttcttcggc tcgtctttca gcaggcgctt
     1561 gagcagagcc agcagctgct gttcgtcttc gccgtgcgca tgatagttca gcaccgtcgc
     1621 atctttttcc gcttcgtagc cgaggtgaat catcagagtg gcatcaccac cgtcgtctac
     1681 gatcagcgtg gggccggaac catcaggcca ggtcaaggct tcattggtgc accaccagta
     1741 ttcctccagg gtctcaccct tccaggcgta gacgggagtg ccggctttcg cgacggcagc
     1801 ggcagcggaa tcaacggtcg aaaaaatatt acaggagcac cagcgcacat cagcgccgag
     1861 ttccttcaga gtttcgatga gaacagcggt ctgcacggtc atgtgcagcg accccatgat
     1921 cttcgcgccc ttgagcggct tgatctggcc atacttttcg cgcagagcca tcaggcccgg
     1981 catttctttc tcggcgactt caatttcttt gcgaccgagt tcggccaggg aaagatcggc
     2041 gactttgtac ggcagacgct tggtgttcac agtggttttt gtctcctgtt ccagggcggt
     2101 ggcgtgctta tcggacttcg gcataacaac tccttgctga ttcgcttggg ccacggctgc
     2161 ctgaaacagc cacgaaagcc cgtttagaat ggtttatgtt ctcacagaac gggccgaaag
     2221 tcaaaagtgg tatactggtc atcatgagat tgtcgcggac atggttgagt tggtatctct
     2281 ggggatgttc ggcactgacc ctcggtgcct tctggttgat ctggtactat gcctggcgca
     2341 atacgtccgg ccataccggc attccgctgg caccgaccac ctggcttttc ctgttcatgg
     2401 cagtcaccgg gctgctgacc cgcacggctc tggggaaagg cctcgacgcc cgccgtccgg
     2461 cgcggctgtt gatcctgtgc ggcggcctgg gctccgcctt cccgaccttt ctcgctcagt
     2521 ttcagctcct gagcccctac gaaacctggc tgaaaaatgg acaaccgctc gtgcacgccc
     2581 ggcgtgacga acttctg
//
LOCUS       DAORHD010000695         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47437_length_2596_cov_3.916568, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000695 DAORHD010000000
VERSION     DAORHD010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47437_length_2596_cov_3.916568"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1693)
                     /locus_tag="PKO06_14190"
     CDS             complement(<1..1693)
                     /locus_tag="PKO06_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV70847.1"
                     /translation="MIIVLLGCAAGCYADQILARGEIREDEGFLKVEFSFDPAGGPVK
                     GTITLDSTGPNLVTETIDWYGKKQEVTKKIGEARTQVNLTFVATLQGSRMTGKTSGSM
                     TYETIPERRLKHSPVVAPGQPRPAGGDEYVTVGKGTKTTVSLEGQAIEAEFDFAAGKA
                     GGFLSYPRAELMKSSGGNEPTSDRIPWEAAFPPQKVAAPAEIPVVIKDGWKGVAADGV
                     SVLELIGTLPQGFVLKSGAAMLVARVRESERKTELKLESAGTAELRVRFEAPSVIDEP
                     FSARVDVSGLMTDTTGATRPFHGRAAFDVVQPPVGLVHGVWSNREAWENGLRSLNAMR
                     YDVVSRFNYAGQNNGNPETIAGQLQQWLDSTTEGPFALAKHRGILASKVDLVGHSLGG
                     LIVRRLVADDPGQNKGLYKRVRRVVTVGTPHMGAPFADWFIYFARSRKKPTDEDRELH
                     PDEVLAHPYWLKGYRDWNPESFHWLVKTVREKKKLDNSFFQDGDAVFGIDGEVDRLGT
                     QFREPENLGVHIGAAAELDASEAGATEFFEVLTGSGLQLHRIGCAVVQGAHGAQAE"
     gene            complement(1805..>2596)
                     /locus_tag="PKO06_14195"
     CDS             complement(1805..>2596)
                     /locus_tag="PKO06_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HNV70848.1"
                     /translation="LLTARGIPTLCYGDETPLVSTSGVFGTNRPDMKFTTESETRELV
                     KSLISLRKEHPALTAGVQQHLGMADSWYAFSRLLPDQAAIVAINNSTHSVRLSLPLPG
                     RVREPAVVTDLLTLAEGRVCRDKLNVQVAPREAVVFIVHPERENGFADAHRLAEAAGR
                     DLYAHGRVRVTFRLTTPPAAVGDVYLCGALPALGAWNPDEAVPCVRDSRGRWSAAVDL
                     PARAVTDFKFFVKAGGKVIWPDGWWNYTLEVPTSRRLTVEKPWMQ"
BASE COUNT          511 a          859 c          721 g          505 t
ORIGIN      
        1 actcggcctg cgcaccatga gcaccctgca cgacggcaca ccctattcga tggagttgca
       61 ggccgctgcc tgtgagaact tcgaaaaact cggtggcacc tgcctcggac gcatccagct
      121 cggcggcagc gccgatgtga acgccaaggt tctctggctc tcgaaactgc gtaccgaggc
      181 ggtctacttc cccgtctata ccgaagacgg catcgccatc ctgaaagaat gaattgtcca
      241 gtttcttttt ctcgcgaacc gtctttacca gccaatgaaa cgattccgga ttccaatccc
      301 ggtatccctt tagccagtag ggatgcgcca gcacctcatc cgggtgaagt tcccggtctt
      361 catccgtcgg cttcttgcga ctgcgagcaa agtaaatgaa ccagtcggcg aacggagcac
      421 ccatatgtgg ggtcccgacg gtaacgacac ggcgcacgcg tttgtacaag cccttgttct
      481 gcccagggtc gtccgcgacc agccgacgca cgatcaaacc gcccaggctg tgaccgacca
      541 gatcgacctt gctcgcgaga atgccacggt gctttgccag agcgaacggc ccctcggtcg
      601 tcgaatccag ccattgctgt aactgaccgg cgatggtttc cgggttgccg ttgttctggc
      661 cggcatagtt aaaacgggac accacgtcgt atctcatggc attcagcgat cgcaggccgt
      721 tttcccaggc ttcgcgattc gaccagaccc catgcaccag cccgaccggc ggttgcacca
      781 cgtcaaacgc cgcccgaccg tgaaagggcc gagtggcccc cgtcgtatcc gtcatcaatc
      841 cgctcacgtc gacacgagcg ctgaacggtt catcgatcac agacggtgcc tcaaagcgca
      901 cccgcagctc ggctgttcca gccgactcaa gcttcagctc cgtttttcgc tctgattctc
      961 tgacccgcgc caccagcatc gccgctccac tcttcagcac gaaaccctgc ggaagcgtac
     1021 cgatcagttc gagaacactg acgccatcag ccgcaacccc tttccaccca tctttgatga
     1081 cgacaggaat ttcggcaggc gcagccactt tctgcggagg aaacgccgct tcccacggga
     1141 tgcgatcaga ggtcggttcg ttcccccccg aactcttcat caactcggcg cgcggatacg
     1201 agaggaaacc tccggctttg cccgcggcaa aatcaaactc ggcctcgatg gcctggccct
     1261 cgagtgagac cgtggtcttc gtccctttgc cgaccgtgac gtattcatca ccacctgccg
     1321 ggcgcggctg gccgggggca accacgggcg agtgtttcaa cctgcgctcc ggaatcgttt
     1381 cataggtcat cgaaccgctg gtcttgcccg tcatcctgct cccctgcagg gtcgcgacaa
     1441 aagtcaggtt gacctgcgtg cgcgcctcgc cgattttctt ggtgacctcc tgcttctttc
     1501 cgtaccagtc gatcgtctcg gtcaccaggt tcgggcctgt gctgtcaagg gtaatcgttc
     1561 ccttcactgg cccgcccgcc gggtcgaaag aaaactctac tttcaggaat ccctcatctt
     1621 cgcggatttc cccacgcgcc agaatctggt cggcgtagca ccccgctgcg caaccaagca
     1681 gaacgatgat cattacggcc gcgacggcaa ccctcggcgc ggcgcggcac cggagtggtc
     1741 gaaggcgatc agagcgtgtc aggaacatcg ggttattggt acgtgttgat ggttgttctg
     1801 acggctactg catccagggt ttctcgaccg tcaaacgacg gctcgtcgga acttcgaggg
     1861 tgtagttcca ccagccatcg ggccagatca ccttgccccc ggctttgacg aaaaacttga
     1921 agtcggtgac cgctcgagcc ggcagatcga cggcagccga ccaacgacca cgcgaatcac
     1981 gcacacaggg aaccgcctca tcggggttcc aggcccccag tgcgggaagc gccccgcaga
     2041 gatagacgtc acccacagcc gcgggcggtg ttgtcagtcg aaacgtgacc cgcacccgac
     2101 cgtgcgcgta cagatcacgc ccggcggctt ccgccaggcg atgggcgtca gcaaacccgt
     2161 tttctcgctc cggatgcacg atgaacacca cggcttcacg tggcgcgacc tggacattca
     2221 gcttgtcgcg acacacgcgc ccctcggcca gagtcagcag atcggtgacc acggcgggct
     2281 cgcgcacccg accggggaga ggcagagaca gtcgcaccga atgcgtgctg ttgttgatcg
     2341 caacgattgc ggcttgatca ggcaacaggc gactgaaggc ataccaggaa tcagccatgc
     2401 ccagatgctg ctgaacaccg gccgtcagag cggggtgttc cttacgcagc gaaatcaggc
     2461 tcttgaccag ttcgcgggtt tcgctttccg tcgtgaattt catgtcgggg cgattggttc
     2521 cgaatacgcc tgacgtcgac accagcgggg tttcatcccc atagcataac gtcggaattc
     2581 cgcgcgccgt cagcag
//
LOCUS       DAORHD010000696         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47438_length_2596_cov_3.799685, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000696 DAORHD010000000
VERSION     DAORHD010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47438_length_2596_cov_3.799685"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKO06_14200"
     CDS             <1..714
                     /locus_tag="PKO06_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70849.1"
                     /translation="VYDPVNYASKPSFLDILELSSNDSTLPANEKKLYDQFTDNTLWA
                     ARPPLERKKFLAEHFRIESYAESDTNPDGSPRDGDEASFAVRNGWGDAATIFCSEPII
                     EDVEHEIWIWADDNVKFMNYMGKTIPHPYHGIEELSLEFNDPRQKPPMKITKNIVKPA
                     DFWTGPIPVVFREPTTGPGLTPPAPSPVAASTCKAYYPSLTVKAKDYKGNRRELTVLF
                     NVMDQKAAVRVLEQKQLKQ"
     gene            743..1306
                     /locus_tag="PKO06_14205"
     CDS             743..1306
                     /locus_tag="PKO06_14205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV70850.1"
                     /translation="MKQLSLEKLCVIGRQHGISLIELMVALVLVGIAVIPVIPVFTQS
                     YVSSSRQVDQEMAIKIAEATVNQMGAVRFDHLNNPGVGVSVPLRYEVPGGTVNASLNL
                     SGSPGTGSASFKIAKTPYEVGVSVTRLFDAVSNPLVFYYFDAFAGKIASYACPDDFLK
                     IDVLVQYQPGDVPVIASLTSFRANLVQ"
     gene            1339..1794
                     /locus_tag="PKO06_14210"
     CDS             1339..1794
                     /locus_tag="PKO06_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70851.1"
                     /translation="MTVLVISVAMLSLLAVLFTRTTAQRGTHSMLADQTRALVAAKGL
                     LQPAIYKCRVLPAEFFRLNDTTIDAASRSTFLATWMTDFDPTASSSAKLIAQQMPAIS
                     AMGISTFTRVLMQKPGLQYRRDYIHLESWAVCNGQRKVVQELLEMSVTQ"
     gene            1794..2537
                     /locus_tag="PKO06_14215"
     CDS             1794..2537
                     /locus_tag="PKO06_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70852.1"
                     /translation="MSVNRVDPGVPRWRHAFTVIEMIVVALVLSVFVITLMYLYQWIT
                     YSYRSTSWKQERTRETEEFWNLLRKNLEEASNIHEEDSVTLDLSIASCPLHFRTLPSI
                     PADGSLDGDLMAWVRARMNPATRTSEYKLACRLWVQKRQVFLSIKSLSMGPVPTSETI
                     PARAFLNDVSGFIVQATPIRQDPSLGDYADTGGTGVGLIIGTQIEVSILFEPAANTGL
                     GTLRLAQNHKFRVPVEHVVESSGNSSFAY"
BASE COUNT          551 a          674 c          770 g          601 t
ORIGIN      
        1 gtgtatgacc cggtgaatta tgcttccaag cccagctttc ttgacattct tgagctgtct
       61 tcaaatgatt cgacgctgcc ggcgaatgaa aagaagttgt acgatcagtt cacggacaat
      121 acgctatggg ctgcccgtcc tcctctggag cggaagaagt ttctggccga acactttcgc
      181 atcgaatcgt atgccgagtc tgataccaac cctgacggct cgccgcgcga tggcgatgag
      241 gcttcgtttg cggtgcgcaa cggctggggt gatgccgcca cgatcttctg ttccgagccg
      301 atcatcgaag atgtcgagca cgagatctgg atctgggcag acgacaacgt gaagttcatg
      361 aattacatgg gaaaaacgat tccgcatccg tatcacggca ttgaggaact gagccttgaa
      421 ttcaatgatc cgcggcagaa gcctccgatg aagatcacga aaaacattgt caagcccgca
      481 gatttctgga ctggcccgat tcccgtggta ttccgcgagc cgacgacggg gccggggttg
      541 actccgcctg caccatcccc ggttgcggcg agtacctgca aggcgtatta tccgtcgctg
      601 accgtcaaag ccaaggatta caagggaaat cggcgcgagc tgaccgtgtt gttcaacgtc
      661 atggatcaga aagcggccgt acgagttctc gaacagaaac agctgaaaca atgaagagtt
      721 ctggttcagg aatcgaagtc ggatgaagca attgtcgctt gaaaaactgt gtgtgatcgg
      781 tcgacaacac ggaatcagcc tcattgagct gatggtcgcg ctggtactgg tcggcattgc
      841 tgtcatccct gtcattcctg tatttaccca gtcctatgtg tcttccagtc gccaggtgga
      901 tcaggaaatg gcgatcaaga tcgcggaagc gaccgtgaat cagatggggg cggtgcgctt
      961 cgatcacctg aacaatccgg gagtcggagt ttccgtgcct ctgcgctacg aagttccggg
     1021 gggaacggtc aacgcgagcc tgaatctctc tgggagtccg gggacaggtt cggcatcatt
     1081 caaaatcgcc aaaaccccgt atgaggtcgg ggtctccgtg acgcggctgt ttgacgccgt
     1141 atcgaaccct ctggtgttct actactttga tgcgtttgcc ggaaagattg cctcgtatgc
     1201 ctgtcctgac gactttctga aaatcgatgt cctggtacag tatcaaccgg gagatgtccc
     1261 ggtgattgcc agtctgacca gttttcgcgc gaatctggtg cagtgatgaa cagagatccg
     1321 cgccggggga tcgcgatcgt gaccgtgctg gtcatcagtg tggccatgtt gagcctgctg
     1381 gcggtactgt ttacccgcac gacggctcag cgcggaacgc actcgatgct ggctgatcag
     1441 acgcgcgctc tggtggcagc gaaggggctg ttacagcctg cgatctataa gtgccgcgtg
     1501 ctgcctgccg agttctttcg gctgaacgac accacgatcg atgctgccag tcgttcgacc
     1561 tttctggcca cctggatgac ggacttcgat ccgaccgcgt cctcttcggc gaagttgatt
     1621 gcccagcaga tgccggcgat ctctgcgatg ggcatcagta cgtttacgcg tgttctgatg
     1681 cagaaaccag gcctgcagta ccggcgcgat tacattcatc tcgagagttg ggctgtgtgt
     1741 aatgggcaga ggaaagtcgt gcaggaactg ctggaaatga gcgtgacgca gtaatgtccg
     1801 tgaaccgagt cgatcccggg gtgccgcggt ggcgacatgc cttcacggta atcgaaatga
     1861 tcgtggtggc gctggttctt tcggtctttg tcatcacgct gatgtatttg taccagtgga
     1921 taacgtacag ttatcgttct acgagctgga agcaggagcg aacaagagaa accgaagagt
     1981 tctggaatct cctgcgtaag aatctcgagg aagcgtcaaa catccatgaa gaggattctg
     2041 tcaccctgga tctcagcatt gcctcgtgtc ccctgcattt ccgcacactt ccctccattc
     2101 cggctgatgg cagtcttgat ggggatttga tggcctgggt gcgcgcgcgt atgaacccgg
     2161 caacgcgaac ctcggaatac aagctcgcgt gtcggttgtg ggtccagaaa cgccaggtgt
     2221 tcctgagtat caagtcactg tcgatggggc ccgtgccgac cagcgagacg attcccgcgc
     2281 gggctttttt gaacgatgtt tcgggtttca tcgttcaggc gacaccgatt cgccaggacc
     2341 ccagtctcgg agattatgcc gataccggtg gcacgggagt aggactgatc attgggacgc
     2401 agatcgaggt ttcgatctta ttcgaacctg cggcaaacac ggggttgggc acactgcgcc
     2461 tcgcccagaa tcacaagttc cgggttcccg ttgagcatgt ggtagaatcg agcggtaaca
     2521 gctcgtttgc ctactgaaaa cgagcgagga attcttggcg aggttctgcg tgtcgcgact
     2581 ggctcgatgg tgtctg
//
LOCUS       DAORHD010000697         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47445_length_2596_cov_3.164109, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000697 DAORHD010000000
VERSION     DAORHD010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47445_length_2596_cov_3.164109"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2562)
                     /locus_tag="PKO06_14220"
     CDS             complement(<1..2562)
                     /locus_tag="PKO06_14220"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV70853.1"
                     /translation="MSVVSEKTGYPAEMLNLDMDMESDLGIDSIKRVEIMSGFQEKMP
                     NAPVVQPGDLGKFRTLRQIIEFLKPVGTAPTVSLPTPSVSAPVTSSRTAAGTDTGAAP
                     SQIEQTLLQVVSEKTGYPVEMLNPDMDMEADLGIDSIKRVEILSGFQEKVPSAPVVQP
                     GDLGKFRTLGQILLYLKGSPAVGSASAHNVTQSQPVQMVAGASAVVPEKALRRVVLTV
                     QEGGASERPFVKLPAGGVIALTDDGTPLTGLVAKAIEKRGFQTIVVPLDDSNPGVVSD
                     QACGLVILAPVAAKAASASWTTASEEALKKAFFLTQRFAPGLTAQASDQGALLATVSR
                     LDGAFGLKRAGRELDPVWGGLAGLAKTAAREWPEVAVKAIDLDRSLKPEEAVDLLVSE
                     LFVVGPVEIGLQAGKRWVPTEQELVIAEEKPVQSPFQSGDVVIITGGARGVTAEVACA
                     LAEAWKPTLVLCGRSALPGEEPAWLRGVEGEKPMKEAILAQARREGRQMTPKDLEIQY
                     QKVCGEREILTTLQRVRQLGAAVAYKSLDVRREDEVKRVMAEVRAEFGTIRGLVHGAG
                     VLRDRRILEKTRDQFDAVFDTKVTGLRNLLQVLANDDLRVIALFSSVTGRYGRIGQVD
                     YSMANEVLNKSAQKLAAARPECRVVSFNWGPWEGGMVTPSLRKVFHDEGVGLIPLVAG
                     GRFFVRELGAGGREDVEIVVGESLPNSAPSPMKEPPTVSTESILAGVQTGSALPPNTA
                     GKRFFSAFEHQLSLNEDKVLNHHIMNGDPVLPMAMVIELLAHGALHENPGFLFHGFNQ
                     LRILRGVVMHGRSEYTLRVWSTKVRGNEDQPVAHVELRGGPRDREVVHASAELVLT"
BASE COUNT          532 a          861 c          708 g          495 t
ORIGIN      
        1 cgtcagaacc agttcggcag aggcatggac gacttcgcgg tcacgcgggc caccccgtaa
       61 ctcgacatgc gccacgggct gatcttcgtt tccgcgcacc ttagtcgacc agacgcgcaa
      121 cgtgtattcg gaccggccat gcatcaccac accgcgcaga atgcgcagct ggttgaagcc
      181 gtgaaagaga aacccgggat tctcatgcaa agcgccgtgg gccagcagct cgatgaccat
      241 cgccatcggc agtaccgggt cgccgttcat gatgtgatgg ttgagaacct tgtcttcatt
      301 cagagaaagc tgatgttcaa aggcggaaaa aaagcgcttg ccggcggtat tcggcggtaa
      361 cgcagagccc gtctgcacac cagcgagaat cgactccgtg ctcacggtcg gcggctcttt
      421 catcggggac ggcgcactgt tcggcaacga ctctcccacg acaatctcaa cgtcttcacg
      481 cccaccggca cccagttcac gaacgaagaa acggcctccc gcaaccaatg gaatcaggcc
      541 gaccccttcg tcatggaaga ctttacgcag cgaaggcgtg accatgcccc cttcccacgg
      601 gccccagtta aacgagacga cccggcattc aggacgggcg gcagcaagct tctgcgccga
      661 tttgttgagc acctcgttgg ccatcgagta atcgacctga ccgatgcgac cgtaccggcc
      721 ggttaccgac gagaacaggg cgatgactcg cagatcatca tttgccaata cctgcaacag
      781 attgcgcagg ccggtgacct tggtatcgaa aacggcatcg aactgatcgc gagttttctc
      841 gagaatgcgt cgatcgcgca gcacaccggc tccatgcacc agaccacgga tcgttccgaa
      901 ctcggcacga acctcggcca tgacgcgctt cacctcgtct tcgcggcgca catcgagtga
      961 cttgtaggca accgcggccc caagctggcg tacacgctgc aacgtggtga gaatctcgcg
     1021 ttcaccgcac actttctggt actgaatttc aaggtctttc ggcgtcatct gacgcccctc
     1081 gcggcgagcc tgagccagaa tcgcctcttt catcggcttc tcgccctcaa ctccacgcaa
     1141 ccacgcgggt tcttcaccgg gtagcgcact gcgaccgcac agcaccagcg tcggcttcca
     1201 cgcttccgcc agagcgcagg ccacctcggc agtcacgccg cgggccccgc cggtgatgat
     1261 gacgacgtct ccgctctgaa acggtgattg cacaggcttt tcttccgcga tcaccagttc
     1321 ctgctcggtg ggcacccagc gctttccggc ctgcagacca atctcaaccg gtccgaccac
     1381 gaacagctcc gacaccagca gatccactgc ttcttcgggt ttgagcgacc gatcaaggtc
     1441 aatcgccttc accgccactt cgggccattc ccgcgctgcc gttttcgcca gtccggcaag
     1501 acctccccag accgggtcca gttcgcggcc ggcgcgcttc aagccaaacg ccccgtccag
     1561 acgggaaacc gtcgccagca gggctccctg gtcggaagcc tgagccgtca gcccgggggc
     1621 gaaccgttgc gtcagaaaaa aggctttctt cagggcttct tcgctggccg tggtccagga
     1681 tgccgaagcc gctttggcgg caaccggcgc cagaatgacc aggccacaag cctgatcgct
     1741 gaccacgccg gggttcgaat cgtccaacgg cacgacgatg gtctgaaaac cacgtttctc
     1801 gatcgctttc gcaacgaggc cggtcagagg cgttccatca tccgtcagcg cgatgactcc
     1861 ccctgccggc agtttcacga aagggcgttc ggaggccccc ccttcctgca cggtcaacac
     1921 gacgcgacgc agggcttttt ccggcaccac tgcactcgca ccggcgacca tctgtactgg
     1981 ttgagattga gtcacattgt gagcagaagc tgagccaact gctggcgaac ccttgagata
     2041 gagcagaatc tgacccaggg tgcgaaactt gccaagatca cccggctgca cgaccggagc
     2101 cgagggaacc ttttcctgga aacccgaaag gatctcgaca cgcttgatcg agtcgatacc
     2161 gagatcagct tccatgtcca tgtccggatt cagcatctca actggatacc cggtcttctc
     2221 gctgaccacc tggagcagcg tctgctcaat ctgggacggc gcggcaccgg tgtctgttcc
     2281 cgcagcagtg cggctggatg tcacaggggc ggagaccgaa ggagtgggca acgagactgt
     2341 cggcgctgtc ccgaccggct tcagaaactc aatgatctgg cgcagcgtgc ggaacttgcc
     2401 caggtcaccc ggctgcacca ccggcgcatt cggcattttc tcctggaacc cggacatgat
     2461 ttcgacccgt ttgatcgaat cgatgcccag gtctgattcc atatccatgt ccagattcaa
     2521 catctctgcg ggataccctg tcttttcgct aaccacgctc atcaacgttt cttgtacctg
     2581 ttgtcccgaa ctgctt
//
LOCUS       DAORHD010000698         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47528_length_2593_cov_3.576044, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000698 DAORHD010000000
VERSION     DAORHD010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47528_length_2593_cov_3.576044"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /gene="hcp"
                     /locus_tag="PKO06_14225"
                     /gene_synonym="priS"
     CDS             <1..1337
                     /gene="hcp"
                     /locus_tag="PKO06_14225"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017872919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNV70854.1"
                     /translation="EKLDGPATFKPASSLEAMVKQGEEVSIPSRRDKIGEDLSGLQEL
                     LTYGLKGTAAYADHAYILGKEDEAVYAFFHKALDYLAKPSPDLNTLVGLCMECGQVNL
                     KTMELLDAANTGHYGHPVPTKVRVEPVKGKAILVSGHDLLDLEHLLKQTEGKGINIYT
                     HGEMLPAHGYPQLKKYKHLVGNYGGAWQDQAKEFDAFPGAILMTTNCIQKPRDTYKAR
                     IFTCGLVAWPGVTHISDRNFAPVIKAALEAPGFTADGASKEIMVGFGHNAVMSVAGKV
                     IEAVKAGQIKHFFLVGGCDGAKSGRNYYTDFAEKAPKDTVILTLACGKYRFNKMDLGD
                     IGGIPRLLDIGQCNDAYSAVQIAVALAKAFNTDVNGLPLSMVLSWYEQKAVCILLTLL
                     SLGVKNIRLGPSLPAFVTPNVLKFLVEKFNIMPVGNSAADDLKAILGSNFKG"
     gene            1473..2447
                     /locus_tag="PKO06_14230"
     CDS             1473..2447
                     /locus_tag="PKO06_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNV70855.1"
                     /translation="MMTTDSPTIPPRVLSIGVVVLDILTSPVERLPEPEKTLSISHVD
                     FAVGGCAANTAHALSRLGFSVSLLGRIGDDVFGRLIRSALQKECIDLSYLEDDVHTPT
                     STSVVLIDNTGERRFLHRSGASGKLAIPADLPLSQFDFLHLGGCFLVPGCMGVPLVQL
                     FRKAREKGVVTSIDTLWHTGGNWTDILSALPHTDYFMPSLLEAQMITGKQLPEEIARY
                     LHDNGSEKVIIKMGERGAFVSTRQEQQVIPAFRVPEGKLKDTTGAGDAFVAGVLKGIS
                     LGWELTRAARLGNACGALATTHFGGPLMQDRHQLSEYIRKVQEDQSIF"
BASE COUNT          537 a          725 c          781 g          550 t
ORIGIN      
        1 ccgagaagct cgatggtccg gcgacgttca agccggcttc gagtcttgaa gccatggtga
       61 agcagggcga ggaagtctcg attccgtcgc gccgcgacaa gatcggcgaa gacctgagcg
      121 gcctgcagga actgctcacc tacggtctga agggaacagc tgcttatgct gatcacgcct
      181 acattctagg caaggaagat gaggcggtgt atgcgttctt ccacaaggcg ctcgactatc
      241 tggccaaacc gtcacctgat ttgaacaccc tggtcggtct gtgcatggaa tgcggtcagg
      301 tcaacctgaa gacgatggag ttgctggatg cggccaacac cggccactac gggcacccgg
      361 ttccgaccaa ggtacgcgtc gagccggtga agggcaaggc gattctggtt tccggccacg
      421 acctgctcga tctggaacat ctgctgaagc agaccgaagg caagggcatc aacatctata
      481 cccacggaga aatgcttccc gcccacggtt acccgcaact gaaaaagtac aagcacctgg
      541 tgggcaacta cggtggtgcc tggcaggatc aggccaagga attcgacgcg ttccccggcg
      601 ccattctcat gaccaccaac tgcattcaga aaccgcgtga cacctacaag gcgcgcatct
      661 tcacctgcgg tctggtcgcc tggccgggcg tcacccacat cagtgaccgg aattttgcgc
      721 ccgtcatcaa agcggcgctc gaagcccccg gattcacggc ggatggcgcg agcaaggaga
      781 tcatggtcgg tttcgggcac aatgcggtca tgagcgtggc cggcaaggtc atcgaagcgg
      841 tcaaggccgg tcagatcaag cacttctttc tggtcggcgg ttgcgacggc gccaagtcgg
      901 gtcgcaacta ctacacggat tttgccgaga aggcgccgaa agataccgtc attctgaccc
      961 tggcctgcgg aaagtaccgg ttcaacaaga tggatctcgg cgacattggc gggattccgc
     1021 ggctactgga tatcggccag tgcaacgacg cctactcggc ggtgcagatt gcggtcgcgc
     1081 tggcgaaggc gttcaatacc gatgtgaacg gtctgccgct gtcgatggtg ctttcctggt
     1141 atgaacagaa agccgtgtgc attctgctga ccctgctctc gctgggtgtg aagaacatcc
     1201 gcctggggcc gtctctgcca gcgttcgtga ccccgaacgt gctgaagttc ctggtcgaga
     1261 agttcaacat catgccagtg ggcaacagcg ccgctgatga tctgaaggcg attctgggca
     1321 gcaacttcaa gggctgagcg atgaacgcgt gttccccggc tggaatacga tgacgtgagt
     1381 gttctctgat cccccggtcg accgtaggac cgggggatca gtattttcag gaaagtggct
     1441 tgctgagcct ctgaggacgt gatatagtgc agatgatgac aacggattca ccaacgattc
     1501 ctccacgggt attgtctatt ggagtggtgg tgcttgatat cctcacctct ccggtcgagc
     1561 ggcttccgga accagagaaa actctgagca tctcgcatgt ggattttgcc gtcggcggtt
     1621 gtgccgccaa tacggcgcac gcgctcagtc gtctgggctt ttcggtgtcg cttctggggc
     1681 gtatcggcga tgatgtattc ggacgtctga tccggtcagc gttgcagaaa gagtgcatcg
     1741 acctcagcta tctcgaggat gacgtgcaca cgccgacctc gacgtcggtc gtgctgatcg
     1801 ataacaccgg tgagcgtcga ttcctgcatc gatctggagc ctcaggaaag ctggcgattc
     1861 ctgccgactt gccgctgtcg cagtttgatt ttctgcacct gggcggctgc ttcctcgtgc
     1921 ccggatgcat gggcgttccg ctggttcagt tgtttcgcaa agccagagaa aagggagtcg
     1981 tcacctcgat tgataccctg tggcacaccg gaggaaactg gacagacatt ctctcagcgc
     2041 tgccgcatac cgactacttc atgcccagtc tgctcgaggc ccaaatgatc acgggcaagc
     2101 agcttcccga agaaattgcc cgttatctgc acgacaacgg ctcagagaaa gtcatcatca
     2161 aaatgggcga gcgtggtgct tttgtttcga cacgccagga gcagcaggtg attccggcct
     2221 ttcgtgttcc ggagggcaaa ctgaaagaca ccaccggagc cggagacgcg tttgtcgctg
     2281 gcgttctgaa aggcatctct ctcggctggg aactcacgcg cgcggcccga ctcggcaacg
     2341 cctgcggagc gctcgcgacg acccactttg gcggcccact gatgcaggac cgacatcaac
     2401 tgtcggagta cattcgcaaa gttcaggaag atcagagcat cttctgatct gatactgcag
     2461 aggcgagcag ggttgatcct gttcgcggcc gtgtgaccag ggacgttggg cacggcgtta
     2521 ttgcagcttt ttcagttcct gaatcagatt ctccagttct gtggtgaggc tctgaatttc
     2581 ggtggcattc tcg
//
LOCUS       DAORHD010000699         2593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47542_length_2593_cov_2.826241, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000699 DAORHD010000000
VERSION     DAORHD010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47542_length_2593_cov_2.826241"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PKO06_14235"
     CDS             complement(<1..472)
                     /locus_tag="PKO06_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70856.1"
                     /translation="MFLSQNLQNLSVQAAVWGPEQRWLDARNLSNQIIGFANFFENPL
                     IGVGWGDTGGSESSYLGGAAAFGIGYAALMACLLFGSMLMCLKLTVFKRRLPKADRAL
                     ADMFIAWNAAYFAGAAFEGYLIGRSSTPQVMALLFAGIGVHLKDMVAVRAGTEAP"
     gene            482..928
                     /locus_tag="PKO06_14240"
     CDS             482..928
                     /locus_tag="PKO06_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70857.1"
                     /translation="MLWTVDPGDWRNRNMTRTIENLKRQMKFSDGGRGGVVLFHDTLP
                     STAHALDPFLVAMKSHGLLTTAFAGRPVVYDRSFWGTRAMSTFAWRELAPILYLAQFK
                     RPLLHSMIRTDDQAELSPIALLRAQKTGTLRRYILCTLGVSSKQSF"
     gene            complement(908..1588)
                     /locus_tag="PKO06_14245"
     CDS             complement(908..1588)
                     /locus_tag="PKO06_14245"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNV70858.1"
                     /translation="MPRLVLFDIDGTILSAGRSGLKALEQAVIEVLQAPRGLDGISLS
                     GNTDRNVLLEISRRDGSPLPDEETIQRFVLRYAEVLRREIMGTGSLMPGIEAILRRLS
                     ELPHVLIGLVTGNFEVGAEIKLDRFGLRHFFQVGAFGGEHHDRGELVGMAIKRAEAKL
                     AGSIRPEHITMIGDTPNDVKACKPWNIRSLAVATGSHPVNELESAGATWVLPSLEASD
                     EVLERLLG"
     gene            complement(1621..>2593)
                     /locus_tag="PKO06_14250"
     CDS             complement(1621..>2593)
                     /locus_tag="PKO06_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV70859.1"
                     /translation="KLAAIDDPETRGLVGRDPLPELLLAIPIAVPGQAFGVIHVEAFQ
                     DQRVEVDDADLRFFASLTSFMGLALSNADIFLQTRDELTSTKQLSEKEMAEKKQLKEI
                     FSRYTSAELVETIMSNPGSINLGGTRKEATILFSDIAGFTNFSAQLTPEQVVTTMNEY
                     LSAMTEVVLENQGEIDKFIGDAVMARFGVLANLQNPGMSAVRAALGMLEALKKLQEKW
                     AQENRQIFTIRVGIASGPLLAGNIGSERRQEFTVMGTTVNLASRLEALNKELKTTILI
                     DEKTYYQVYQHVRAIPRDDTRIRGLEGTMTVYEVKGLLPVSDGGQRG"
BASE COUNT          525 a          835 c          679 g          554 t
ORIGIN      
        1 tcggcgcttc ggttcccgcc ctcaccgcca ccatgtcctt caggtgcacg ccgatccccg
       61 cgaacagcag ggccatcacc tgcggcgtgc tcgatcgtcc gatcaggtat ccctcgaacg
      121 ccgcgccggc aaagtacgcc gcgttccacg cgatgaacat gtccgccagc gcccgatcgg
      181 ccttgggcag ccggcgtttg aacaccgtca gtttcaggca catcagcatg ctgccgaaca
      241 gcaagcaggc catcaacgcg gcgtagccga tgccgaacgc cgcggcgccg cccagatagc
      301 tcgattcgct gccgccggtg tcgccccaac ccacgccgat cagcggattc tcaaagaagt
      361 tcgcgaaacc aatgatctga ttcgacagat tacgggcgtc aagccaacgt tgttccggcc
      421 cccatacggc ggcctgaacg ctcaggttct gcagattctg cgacaggaac acatggatgt
      481 gatgctctgg accgtagatc ccggcgactg gcgcaaccgc aacatgaccc gcacgatcga
      541 gaatctgaag cggcagatga agttctctga tggaggtcgc ggaggagtcg ttctgtttca
      601 cgacaccctt ccctcaaccg cccacgcgct cgaccccttt ctggtcgcca tgaaatcaca
      661 cgggttgctg acgaccgctt tcgcgggtcg ccccgttgtc tacgatcgca gcttctgggg
      721 cacacgcgcc atgtcgacct ttgcctggcg cgaactggct ccgattctct atctggccca
      781 gttcaaacgc ccgctgcttc acagcatgat tcgcaccgac gatcaggcag aactgtcgcc
      841 cattgctctg ctacgcgcac agaagaccgg cacacttcgt cggtacattc tctgcacgct
      901 tggcgtgtca tccaagcagt ctttctagta cctcgtcgct ggcttccagg ctgggtaaaa
      961 cccaggttgc tccagcactt tcgagttcgt tgacggggtg cgatccggtt gccacagcca
     1021 gagaacgaat gttccagggc ttgcaggctt tcacgtcgtt gggcgtatct ccaatcatcg
     1081 tgatgtgctc ggggcgtatc gaaccagcca gcttcgcttc cgccctcttg atcgccatgc
     1141 ccaccagttc gccccgatcg tgatgctcgc cgccaaacgc cccaacttga aaaaagtgtc
     1201 gcaggccaaa ccggtcgagc ttgatttcgg ccccgacctc gaagtttccc gtcaccaacc
     1261 cgatcagaac atgcggcaac tccgacagtc gccgaagaat ggcttcgata ccaggcatca
     1321 atgagcctgt tcccatgatc tcccggcgca gcacttctgc ataacgcaac acaaaacgct
     1381 gtatggtctc ttcatccgga agcggtgagc catcacgccg cgagatctcg aggagcacgt
     1441 tgcggtctgt gttgccactg agactgatgc catctaggcc ccggggtgcc tgcaacactt
     1501 cgatcacagc ctgctcgagg gctttgaggc cactgcgacc cgccgacaga atcgtgccgt
     1561 cgatatcaaa cagtacaagt cgaggcaaga ccacatcatt tcgtgcagtc tgttccaggc
     1621 tcatcctctt tgtcctccat ccgacacagg caacagcccc ttcacttcat aaacggtcat
     1681 tgttccctcg agaccgcgaa ttctggtgtc gtcgcgcgga atcgcccgca cgtgctgata
     1741 cacctggtag taggtttttt catcgatcag aatcgtcgtt ttcagttcct tgttcaacgc
     1801 ctcgagtcgt gaggccaggt tgacggtcgt gcccatgacc gtaaactcct gccgccgctc
     1861 tgaaccgatg tttccggcaa gaagcgggcc agaggcgatt cccacccgaa tcgtgaaaat
     1921 ctggcggttc tcctgcgccc acttttcctg caacttcttc agcgcttcga gcatgcccag
     1981 tgccgcgcgc accgccgaca tccccggatt ctggagattg gccagaacgc cgaaccgggc
     2041 catcactgcg tcaccaatga acttgtcaat ctccccctgg ttttcgagta ccacttcggt
     2101 catggccgac agatactcgt tcatcgtggt tacgacctgt tcaggagtca gctgtgcgct
     2161 gaaattggtg aatccggcga tgtccgaaaa aagaatggtg gcttctttcc gcgtgccgcc
     2221 caggttgatc gagccggggt tcgacatgat cgtttccacg agctcagctg atgtataccg
     2281 ggaaaaaatc tccttcaatt gcttcttctc ggccatctct ttttcgctca attgcttggt
     2341 cgatgtgagc tcatcacggg tctgcagaaa gatgtccgcg ttgctcaatg ccaatcccat
     2401 gaacgaggtc aatgacgcaa agaagcgcag gtcggcgtca tccacctcga ctcgctgatc
     2461 ttgaaaggct tcgacatgaa tcaccccgaa cgcctggccg ggaacggcaa tcggaatggc
     2521 gagaagcaac tcgggcagag ggtcacgacc tacgagcccg cgtgtttcgg ggtcatcgat
     2581 cgccgcaagc ttg
//
LOCUS       DAORHD010000700         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47621_length_2590_cov_3.671006, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000700 DAORHD010000000
VERSION     DAORHD010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47621_length_2590_cov_3.671006"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2590
                     /locus_tag="PKO06_14255"
     CDS             <1..>2590
                     /locus_tag="PKO06_14255"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV70860.1"
                     /translation="NSSRHLFTASLLRLLLLAGLLVIPGLMVFFGMTAREERMLEQMR
                     RRLLHEATSRLDRYVHLGDRGIYLAQLLRETFDRADGGPNPVDELSRRLNRVKRSFPE
                     ALTTIAWNASGSLIPACTDIQGFRTILAKTHDILRETALHIREIPARELPMLPGIAPS
                     LSLIGGFFGRFFVPSQLAWAYGIESGYPLALAEVGTRRSYYWHRSGKHLSLLVFLNEA
                     VVQNHRGFEKTIAAVNRRSPHTWQLTIVDPRSPHLLTASAPSGHLCELLTTVQKETHA
                     GGQLHETQNLIVGTRRLPDGRILLAWIPRTHKLPHLPRRIRQLMVNIALGYILLFLVW
                     KCSVWRRWIAPSIQYELVLLFLMASGVPLGTLIINSSEFLETWRDSTVRHLQEEAERR
                     FKQADDRVTPFVNQFAERLGADLPHISPASNASFTPQEVFSRLASSPTISSANELYLF
                     AEGGINLTACPGRLVSPARKNSMTEIGKAVRSMLIYGSLPSSGRGKGEGITDTRQFLN
                     AVGLLMLKVGGLRRLEIGSRKEWLYVNEIRAGPQPRDWMLLVGVWSDQSIAEMFLHRT
                     LSDLRQLLPEIRVEAHPGKEMGTWLTSRIHEHWAETLNLARQRGRVLFQNVAREGRFW
                     YGLAYASRVVPDLVYLATISESQISNAQQQLLKWGTAAGLVLFAFALLIGGGLARSFL
                     KPLERIENTAIALQNRQFTCPPVSGSLDEFGELERTFAEVTGSLSELEIARVVQESLL
                     PDDQLSFGPFKVYGRSCAVTALGGDYFDFFVHKDSKICVLLGDVAGHGVGAALMMAIA
                     KAAVFLGRASWDKPSAVLQLVHEQFFALQKKPKKLMTMLFVTLDVGTGAGWIANAGQT
                     YPILLRQ"
BASE COUNT          524 a          784 c          711 g          571 t
ORIGIN      
        1 aattcttcgc ggcatctttt cactgcttca ctgctgcgcc tgctccttct ggcggggtta
       61 ctggtcattc ctggcctgat ggtgttcttt gggatgacgg cacgagagga acgaatgctg
      121 gagcagatgc gcagacgtct gctgcatgaa gcaacgtcgc gactggaccg gtatgttcac
      181 ctcggcgacc gcgggatcta tctggcacag ctgctgcgcg agacctttga ccgagcggac
      241 ggtggcccca accctgtcga cgaactgtcc cgacggctca accgtgtcaa gcgaagcttt
      301 cccgaggctc tgacaacgat cgcctggaat gccagcggtt ctctcatccc cgcctgtacc
      361 gatatccagg gttttcggac aatcctggcg aagacccatg acattctacg cgagacagcc
      421 ctgcacatcc gggaaatccc ggcgcgcgaa ctgccgatgt tacccgggat cgctccatcg
      481 ctgtcgctga tcggaggatt tttcggtcgt ttttttgttc cgagccagct ggcctgggcc
      541 tatgggatcg agagcgggta tccgctggcg ctggctgaag taggtactag gaggtcgtat
      601 tactggcatc gatcaggaaa gcatctcagc ttgttggtat ttctcaacga agcggtggta
      661 caaaaccatc ggggcttcga gaagaccatt gccgcagtca atcgccgatc gccgcatacc
      721 tggcaactca cgattgttga tccgcgttcg ccgcatcttc tcaccgcatc tgctcccagc
      781 ggtcatcttt gcgaactgct caccacggtg cagaaggaga ctcatgcggg aggtcagttg
      841 catgaaaccc agaacctgat cgtcgggacc cgacgtctgc cagacggacg aattcttctc
      901 gcctggatac ctcggactca caaactgcca catctccccc gtcggatccg gcaactcatg
      961 gtcaacatcg ccctcgggta cattctcctc tttctcgtct ggaagtgcag cgtgtggcgc
     1021 cgttggatcg caccatcgat tcaatatgaa ctagtactcc tgtttcttat ggccagcggt
     1081 gtgccactcg ggactctgat catcaactcc tccgagtttc tcgagacctg gcgggattcg
     1141 accgttcgcc accttcagga agaagcggaa cgcaggttca aacaagccga cgaccgagtc
     1201 acccctttcg tcaatcaatt tgctgaacgt cttggcgcgg atcttcccca catctctccg
     1261 gcatcgaacg cctcgttcac ccctcaagaa gtgttttccc ggctcgcctc atcacccacc
     1321 atctcctctg caaacgaact gtatctgttc gcggaaggcg gtatcaacct gacggcctgc
     1381 ccaggtcgac ttgtctcacc agcccggaaa aacagtatga ccgaaatcgg caaagcggtt
     1441 cgatccatgc tgatctatgg ttccctgccg tcttcaggtc gcggaaaggg agaaggaatc
     1501 accgatacca gacagttcct gaacgccgtc ggtctgctga tgctgaaagt cggaggactc
     1561 agacgtctgg agatcggctc gcgaaaggaa tggctctacg tgaacgagat tcgagcgggc
     1621 ccacagccgc gtgattggat gcttctggtc ggggtctggt cagatcagag catcgccgag
     1681 atgtttcttc accgcactct ctcagacctg cggcaactcc tgcctgagat ccgtgtcgaa
     1741 gcgcaccccg gcaaagaaat gggaacctgg ctcacgagcc ggatccacga gcactgggca
     1801 gagaccctga atctggcccg acagagaggc cgagtgctct ttcagaacgt ggccagagaa
     1861 ggtcgtttct ggtacggcct ggcatatgcg agtcgagtcg tacccgatct ggtctatctg
     1921 gcgacgatct cggaatccca gatctccaac gcccagcagc agctcctgaa atggggcact
     1981 gccgccggcc tggtcctctt cgctttcgcc ctgctgatcg gaggtggtct cgccagatcg
     2041 tttctgaagc cgctcgagcg catcgaaaac acggcgattg ccctgcagaa ccgacagttc
     2101 acctgcccgc cggtcagcgg ctcactcgat gagttcggag aactggaacg cacgtttgcc
     2161 gaagtgaccg gaagtctctc ggagttggaa atcgctcgcg ttgttcagga gagcctgctt
     2221 cccgatgatc agctctcatt cggccctttc aaggtctacg gtcgttcatg tgccgtgacc
     2281 gctctgggcg gtgactactt cgacttcttt gtccacaaag acagcaaaat ctgcgtcctg
     2341 cttggcgatg ttgccggaca tggcgttgga gccgcgctga tgatggcaat cgcaaaagcc
     2401 gccgtctttc tcggacgcgc ctcctgggac aaaccatccg ccgtgctgca gcttgttcac
     2461 gagcagtttt tcgccctgca gaaaaaaccg aaaaagctca tgacaatgct tttcgtcacc
     2521 ctcgatgtgg gaaccggcgc aggatggatt gccaatgccg ggcagaccta cccgatactg
     2581 cttcggcagg
//
LOCUS       DAORHD010000701         2584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47801_length_2584_cov_3.000000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000701 DAORHD010000000
VERSION     DAORHD010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47801_length_2584_cov_3.000000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKO06_14260"
     CDS             <1..1104
                     /locus_tag="PKO06_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV70861.1"
                     /translation="WSELSSELLLSVRESLAICRTAGIQVKMITGDHLATAHAIAKDL
                     GISQAGTEGVSGKELAQTSNEALPEVAEKSSVFARVAPEHKFRLVEALQKHGHVVAMT
                     GDGVNDAPALKRADIGVAMGMAGTDVAKEAADMVLVDDNFATLVRAVEEGRNVFNNLV
                     KSMVYILPTNTAQGLVLLFAVLLGMRLPITPVQVLWVNLLTAFLSLPMAFEAMEPGLM
                     KRPPRHPEAPLMDRRLIIRISLVALVMTFLSFVIYWYERQLGSDIARARTAVTATMVA
                     VEFFYLFPSRGLWAPMPGLSPTLNPVLWPCLLATVALQLAFTYVPMFQNLMGSRPIYL
                     ESWLVILAVSATVIPLIEMLKRWTGTDGETGRV"
     gene            complement(1170..1907)
                     /locus_tag="PKO06_14265"
     CDS             complement(1170..1907)
                     /locus_tag="PKO06_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70862.1"
                     /translation="MSALAISRNRPIHLLLIFSMMASFLVLPAPSAKAGMLGSLLSTL
                     RPYLGIAGKIGGAVIGASMCSAFCPPLGMIAGGIAGWIAGGIITGTATASLGNLAAVG
                     CGVVGAMALGPGAIGMIGGFLLGAFLGKTAVGLLQKADRSITGGILLSPAGVSNSNAF
                     SNSGSVSISANSGSTAAAAGSSLTPVNANQTTSVTEQLKAAQERYQKAYTDYTTATQV
                     GDTQGAAELHKTYLSAYQEYQNLLRSK"
     gene            complement(2038..>2584)
                     /locus_tag="PKO06_14270"
     CDS             complement(2038..>2584)
                     /locus_tag="PKO06_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70863.1"
                     /translation="LSLEPDLSPLMRERLGFLLSRYVLDQETSIKSGRQESEGQTFTR
                     TFLTKPGALSIRFERGKRKPAERETTTRRRREPKSEPQATTLDPPAETDSEESSLYLE
                     AYANRPEDWLTSITLNLIELPGMTLVGKGRIVGREEEGKGWLFRSEDISLLHKRGRYL
                     AILEYTNSDNDKSYQGLLFGI"
BASE COUNT          526 a          688 c          796 g          574 t
ORIGIN      
        1 tggtcggagc tgtcgagcga gctgctgctc tccgttcgtg agtcgctggc catctgccgc
       61 acggccggaa ttcaggtgaa aatgatcacg ggtgaccatc tggcaaccgc gcacgctatt
      121 gccaaagatc tggggatcag ccaggcgggc accgaggggg tttccggcaa ggaactggct
      181 caaaccagca atgaagccct tcctgaagtg gcagagaaat cgagtgtctt tgcccgtgtg
      241 gccccggaac acaagttccg cctggtcgag gcgctgcaga agcatggaca tgtcgtggcg
      301 atgaccggtg acggagtcaa tgatgctccc gcgctgaagc gcgctgatat tggggtggcg
      361 atgggaatgg ctggtacaga tgtggcgaag gaagccgcgg acatggtgtt ggtcgatgac
      421 aactttgcca ctctggttcg cgcggttgaa gagggacgca acgtcttcaa caacctggtc
      481 aagagcatgg tgtatattct gccgaccaac acggcacagg gcttggtgct gttgtttgcc
      541 gtgctgctcg gcatgcgtct gccgattacg cccgtacagg tgctgtgggt gaatctgctc
      601 acggcgtttc tctcactgcc gatggctttt gaggctatgg agccagggct gatgaaacga
      661 cctccccgcc atccagaggc tccgctcatg gaccgtcgtc tgatcattcg tatcagtctg
      721 gtggcactgg tcatgacgtt tctaagtttt gtcatctact ggtacgaaag acagcttggg
      781 tcggatattg cccgcgcgcg taccgcggtt accgcaacta tggtggccgt cgaattcttc
      841 tacctgtttc cctcacgggg attgtgggca ccgatgccgg ggctttcacc cacactgaac
      901 ccggttctct ggccatgtct gttggcgacg gtggctctgc aactcgcttt cacctatgtg
      961 cccatgtttc agaaccttat gggcagccgt ccgatctatc tcgaatcctg gctggtgatt
     1021 ctggcggttt cggcgacggt cattccgttg atcgagatgc tgaagcgctg gacaggcacc
     1081 gacggagaaa ccggtcgcgt ctgatcttct tcgcggacaa agaaaaaccg gctgaactca
     1141 gttcagccgg tttttggcct tcggacaagt tacttgctgc gcaacaggtt ctggtattcc
     1201 tgatatgccg agagataggt cttgtgcagt tcggccgctc cctgtgtgtc gccgacctgt
     1261 gtcgccgtgg tgtagtcagt gtaagctttc tgataccgtt cctgggcagc tttgagctgt
     1321 tcggtgaccg atgttgtctg attggcgttc accggagtga gggatgagcc ggcagcagct
     1381 gcagtcgagc cagaattggc cgaaatcgac acggaaccag agttggagaa ggcgttggag
     1441 ttggacacgc cggccgggga gagcagaatt ccgccggtga tcgagcgatc tgccttctgc
     1501 agcagtccga ctgcggtttt gccaaggaat gctccaagca ggaagccgcc gatcatgccg
     1561 atcgcgccag gccccagagc catggcacca acgacaccgc aacccacggc cgctagattg
     1621 ccaaggctgg ctgtcgcagt gcccgtaatg attccaccgg cgatccagcc ggcgataccc
     1681 ccggcgatca tgccgagcgg cggacagaaa gcggaacaca tggaagcacc aatgacggcg
     1741 ccaccgattt ttccggcaat tcccagatag ggacgtaacg ttgagaggag ggaccccagc
     1801 atcccggctt tggcgctcgg agcgggaaga acgaggaacg aggccatcat cgagaagatc
     1861 agaagcagat gaatcggtcg attgcgggag atggcgagtg ctgacatacg aaaacctcct
     1921 ccgaaaacga tttcgtcacg atatgtagaa attgtagcag acaaaacgcg taccccgcaa
     1981 gagaagcttg ggaaggactc ttgcgtgggg cgttaagcga cggatgctga caggcagcta
     2041 gatgccgaag agaagaccct gatatgactt gtcgttgtct gagttcgtat attcgagaat
     2101 agccagatac ctgcctctct tgtgaagcag gctgatatcc tcgctgcgga acaaccagcc
     2161 cttgccctct tcttcgcgtc cgacaatgcg gcctttgccg accagggtca tgccgggcaa
     2221 ctcgatcaga ttcagggtaa tgctggtcaa ccagtcttcg ggacggttgg catacgcctc
     2281 aagatacaga ctgctttcct cgctgtcggt ttctgcgggt gggtcaagag tggttgcctg
     2341 tggttcagac ttgggctcac ggcgtcgtct ggtcgttgtc tcacgttccg ccggtttccg
     2401 cttgccgcgt tcaaatctga tagacagggc cccgggtttg gtcagaaagg tgcgggtaaa
     2461 ggtttggcct tcactttcct gtcgtccgga tttgatggag gtttcctgat cgagaacata
     2521 ccggctgagc aaaaaaccca ggcgttcgcg catgaggggt gagagatcgg gctcgagact
     2581 cagt
//
LOCUS       DAORHD010000702         2582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47841_length_2582_cov_4.239810, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000702 DAORHD010000000
VERSION     DAORHD010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47841_length_2582_cov_4.239810"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKO06_14275"
     CDS             complement(<1..546)
                     /locus_tag="PKO06_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70864.1"
                     /translation="MITRLEATRYRCLERLDADLGEFALVVGANGAGKTTFLDLPVLL
                     GDLLTQKDAAAAFSSRLRGLPPRCGALSELVFCGRGDDFILAVEAALPDTVVRELLPS
                     RSGPVQNQDEWWPRFIRYELRLQVFNQRSLYVDGEYLFLFSRASVPDRDQFRLLGEHA
                     RKKDWRFVIDRAPGKDALFRSE"
     gene            complement(543..2582)
                     /locus_tag="PKO06_14280"
     CDS             complement(543..2582)
                     /locus_tag="PKO06_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015080841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNV70865.1"
                     /translation="MGKRKHIAAEEVAEQTAAENDEVLSLDEGTVLDYITNKPLKDTP
                     KEQVRQRIARALFHEYALSVDDMEPDFKMTIDGRRKKIDIAVFEHDAPHDVEHLRRVV
                     ICEKEPTTGRKSAYKMRTPEEAKKEYALLHEAMAEASGCKFGLWTNGLEFFFFEKETT
                     KFDVKFKSIGSWPMADESQGTRDVISTARLRRADPEMLRIAFRRCHNFIHGNEGMSKD
                     AAFWQFLYLIYCKMHDERQSGDLRRFWAGPTEQYDAAGRQEIKKRISSLFGEVKKKYK
                     SIFRGNEEITLSDRALSFMVSELAKYDFGRTDVDAKGAAYQEIVGNNLRGDRGQYFTP
                     RGAIKLMVQILDPKSNERLLDPACGTGGFLVGAVGHLFEKFKSETPSNGEETADNLVA
                     AEQIRHYVEDKLFGCDFDPALVRATTMNLMMAAGSSGNVFHLDSLAFPEGHLDGLKEA
                     RESVPLGTIDVLMTNPPFGSDIPITDPGILGKYDLAAKWEKAEAGGYKRGTGYQSAVA
                     PEVLFVQRAVEWLKPGGRMGIVLPDGILGNPGDEPIRQWILRNCWVLASVDLPVETFI
                     VEANVNILTSLLFLKRKTQAEIQTEDLGKKTDYPVFMAVAERVGVDRRGNILYKRKPD
                     GEEEWVDYEDSEKIRTGGKMVVRKLVRKKRIVDDDLPLIAAAYREFRRKNPEPGA"
BASE COUNT          529 a          740 c          716 g          597 t
ORIGIN      
        1 ttcactgcga aacagtgcgt cttttccggg ggcgcgatcg atgacgaatc gccaatcctt
       61 tttgcgtgcg tgttccccaa gaagtcgaaa ctggtctcga tccggtacgg atgcgcgaga
      121 aaacaaaaac aggtattcgc catcgacata cagagaacgc tgattgaaga cctgcaatcg
      181 cagttcatac cgaatgaagc gcggccacca ctcatcctga ttttgcaccg ggcctgatcg
      241 actcgggagt agttcacgca cgacggtatc tggcaacgcg gcttcaaccg cgaggatgaa
      301 atcatccccg cgcccgcaga acaccagttc tgacaacgcc ccacaccggg gcggcaggcc
      361 gcgaagacgg ctggaaaaag cggcggcagc gtctttctgg gtcaggagat cgcccaagag
      421 caccggcaga tcgagaaacg tggtcttgcc cgcaccgttg gccccgacga ccagagcaaa
      481 ttcaccgagg tcggcatcga gtcgctcgag gcaacgatag cgtgtggctt cgagacgagt
      541 gatcatgcgc ccggctccgg gttttttcgc cgaaactcgc ggtacgccgc ggcgatgagc
      601 ggcaggtcgt catcgacaat gcgcttcttg cgtaccagct tgcgaacgac catcttcccg
      661 ccggttcgaa tcttttcaga atcttcgtag tcaacccatt cttcttcgcc gtccggcttg
      721 cgtttgtaca agatgttccc tcggcgatcg accccgaccc gttcagcgac ggccatgaat
      781 acggggtaat cggtcttctt gccgaggtct tcggtttgaa tctctgcttg ggtcttgcgt
      841 ttgagaaaaa gcagactggt caaaatattg acgttcgcct cgacgatgaa ggtttcgacc
      901 gggagatcga cgctggcgag cacccaacag ttgcgcaaga tccattgtcg aatcggttcg
      961 tcgccgggat tgccgagaat cccgtcgggc agcacgattc ccatgcgacc gccgggtttc
     1021 agccattcga ccgcacgttg aacgaacaga acctcaggcg ctacggctga ttgatagccg
     1081 gttccgcgtt tgtagccacc ggcttcggct ttttcccatt tcgccgcgag atcatacttg
     1141 cccaggattc ccgggtcggt gatgggaatg tcagacccga acggcgggtt cgtcatcaac
     1201 acatcgattg ttcccaacgg aacggactct cgcgcttctt tgagcccatc gagatgtcct
     1261 tcgggaaagg ccaacgagtc gagatgaaag acgttccccg acgagccggc ggccatcatc
     1321 agattcatgg tcgtggcgcg aaccagcgcc gggtcgaagt cacagccgaa caacttgtct
     1381 tcgacgtaat ggcgaatctg ctcggcggcc accagattgt ccgcggtttc ctcgccgttc
     1441 gaaggggttt cgctcttgaa cttctcgaac aagtgcccga cggcaccgac gagaaagccg
     1501 ccggtgccac aggcggggtc gagcagtcgt tcgttcgact tcgggtcgag tatttgtacc
     1561 atcagcttga tggctcctcg aggggtgaaa tactgtcccc ggtcacctcg aagattattc
     1621 ccgacgattt cctgataagc cgcgcccttg gcgtcgacat ccgtgcggcc gaagtcatac
     1681 ttggctaact cggaaaccat gaaagacaac gcgcgatcag agagcgttat ttcctcgttt
     1741 ccgcgaaaga tcgacttgta ctttttcttc acttcgccga agagggaaga gatgcgcttc
     1801 ttgatttctt gtcgcccggc ggcgtcatac tgttccgtcg gtccggccca gaagcggcga
     1861 aggtcgcccg actgtcgctc gtcatgcatc ttgcagtaga tgagatagag aaactgccag
     1921 aacgcggcat ctttcgacat gccttcatta ccgtgaataa agttgtgaca gcgccggaag
     1981 gcgatgcgca acatctcggg gtcggcgcgc cgcaatcgag cagtggagat gacatcccgc
     2041 gttccttgac tttcgtccgc catcggccac gagccgatgc tcttgaactt cacatcaaac
     2101 ttggtcgttt ctttctcgaa gaagaagaat tcaaggccgt tggtccagag tccgaatttg
     2161 catccggatg cttcggccat ggcttcatgt aacagggcat actctttctt tgcttcttcc
     2221 ggggtgcgca ttttgtaagc gctcttgcgc ccggtggtgg gctctttttc gcagatgacg
     2281 acgcggcgca gatgctcgac atcatgcggt gcgtcatgct cgaagacggc aatatcgatt
     2341 ttcttccgcc gaccgtcgat cgtcatcttg aaatcgggtt ccatgtcatc gacggaaagg
     2401 gcatactcat ggaataatgc tcgggcaatt cgctgccgca cttgctcttt gggcgtgtct
     2461 ttcagcggct tgttggtaat gtaatcgaga acggttccct catcgagtga gagaacttcg
     2521 tcattttctg ctgcggtttg ctctgcaacc tcttctgccg ctatatgctt gcgcttgccc
     2581 at
//
LOCUS       DAORHD010000703         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47879_length_2581_cov_2.554632, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000703 DAORHD010000000
VERSION     DAORHD010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47879_length_2581_cov_2.554632"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKO06_14285"
     CDS             complement(<1..702)
                     /locus_tag="PKO06_14285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70866.1"
                     /translation="MKRCHLLIVFLLLVSLCQALDAADTPEATEQYKYALGLIQRQMY
                     EEASVVLQRVLSDAEPFSKRDAAMYWFAECHYRLGRFNEAIGAYQKMRQEFPASTMAD
                     RAAYGLGWAHARNNDPKSAVEAFNRVTPADGKLWIDARLKMGLLMVKFNMDAETTAKV
                     YEQLLSRKDLTDAQRFEALFQAGVLRFNQTSYQTAVGHFTDALAKAPAEQLTSVLFFL
                     AESHFRQRAYKDASSY"
     gene            complement(752..982)
                     /locus_tag="PKO06_14290"
     CDS             complement(752..982)
                     /locus_tag="PKO06_14290"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV70867.1"
                     /translation="MGLNFSELIVILVIVLILFGPGKLPDIGRALGRGIREFKKAQKD
                     ALDGDDPEPPPSPPQSKNVESGDRTGSSDKNI"
     gene            981..>2581
                     /locus_tag="PKO06_14295"
     CDS             981..>2581
                     /locus_tag="PKO06_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70868.1"
                     /translation="MGPPVPVLRVLATACGEGGAIVSWGLFSFKASPGKGPLNMQLRR
                     LLYLTMAAVFTSSWLLGQATVTERLSSPTASATAVATAVTVEASASPALSDPEAMTLE
                     LTLTALHRLHQRAEAGLRLLKAAESTTALFQRNPQTERAALDESLGGYTRKLEEKGWQ
                     SAYESLGLDIKILESRRDMLQSRYLGIERAFQDSQEQLRRISADLAENRTLLNDADQR
                     LKALQEQLKVADAPGTRAKLALLQEVRTTRESYQRLLQEMQSVLQNRTQRTRQDLGIG
                     SETLSVYQTLLPRYSQLLNQYEEKVARTNTRNLEEKAQSGLEGIRREQATLQESLDAL
                     EQQMLQVATTSTRTALTLQREVLVTQREALDESGRLFEYQLRQSRIAQQFGPILRPGG
                     LEGMPREQILRTRQETNEWLQSLRQDLKLAEKRKELFQANRQLLEKAIADLESRTGSA
                     GIASRDEIKARRQQLKHQTEAGRKCVEQEHLLETLIGEVERVQATMTEHHLRKREEEL
                     FGRRTLAIRPNLPTLVEETLRRLPAV"
BASE COUNT          579 a          784 c          760 g          458 t
ORIGIN      
        1 gtagctcgac gcgtctttgt aggcgcgttg gcgaaagtga gattcagcaa ggaagaagag
       61 gaccgacgtc agttgctcag cgggagcttt agccagcgca tcggtgaaat gtccgaccgc
      121 cgtctgatac gaagtctgat tgaaacgcaa cacgccggcc tggaaaagcg cttcgaatcg
      181 ctgggcgtcg gtcagatctt tgcgtgacag cagctgttcg tagactttcg ccgttgtctc
      241 cgcatccatg ttgaacttga ccatcagcaa ccccatcttc aaacgcgcgt cgatccagag
      301 cttgccgtcg gcgggagtca ctcgattgaa ggcttcgaca gccgacttgg gatcattatt
      361 gcgggcatgg gcccagccga gaccgtaggc ggcgcgatcg gccatcgtgc tggcagggaa
      421 ctcctggcgc attttctgat acgcaccgat ggcctcattg aagcgcccca ggcggtagtg
      481 gcactcggcg aaccagtaca tggccgcgtc gcgcttcgag aacggctcgg catcagagag
      541 cacgcgctgc aacacgacac ttgcttcttc atacatctgg cgctggatca agccaagagc
      601 atacttgtac tgttcggtcg cttcgggcgt gtcagccgca tcgagagcct gacacagact
      661 cacaagcagc agaaaaacaa tcagcagatg gcaacgtttc atgcgtgcgg atctcctcgc
      721 agtttcgacc accagcaaaa atcagttggg atcagatatt tttgtcggac gaacccgtac
      781 gatcaccgga ttcgacattc ttggactgag ggggtgaagg cggtggctca ggatcatcac
      841 cgtcgagcgc atctttctgc gccttcttga actcgcgaat accacgaccg agcgcccgcc
      901 caatatcggg cagctttccg ggcccgaaca gaatcagaac gatgacaaga atcacaatca
      961 attcggaaaa attgagcccc atgggtcctc ccgttccggt gttgcgggtg ctggccaccg
     1021 cttgcggcga aggcggagca attgtatcat ggggtctctt ctctttcaag gcatcaccgg
     1081 gaaagggccc cctgaatatg cagcttcgta gacttctgta tctgacgatg gcggccgttt
     1141 tcacgtcatc gtggcttctc ggacaggcga ccgttaccga gcgactctct tcccccactg
     1201 cgagcgccac cgcggtcgcg accgccgtga cggtcgaggc ctcggcatcg ccggcactca
     1261 gcgaccccga agctatgact ctcgagctga ccctgaccgc tctgcatcga ttgcatcagc
     1321 gtgccgaggc cggtttgcgc ctgttgaaag ccgcggaatc gacgaccgcc ctgtttcagc
     1381 gcaaccctca aacggaacgc gccgcgctgg acgagtcgct tggcggctat acccgcaagc
     1441 tcgaagaaaa gggctggcag agcgcgtatg aatcgctcgg cctggacatc aagattctcg
     1501 aatcgcggcg cgacatgctg cagagccgat atctcggcat cgaacgagct ttccaggatt
     1561 ctcaagagca gctgcgccgc atcagcgccg atctggccga aaaccgtacc ctgttgaacg
     1621 acgccgacca gcgcctcaag gcactgcagg agcagctgaa agtcgcagat gcgcctggaa
     1681 cgcgcgccaa actggcactg ctgcaggaag tgcgtacgac gcgtgaatcc taccagaggc
     1741 ttctccagga gatgcagtcg gtgctgcaga accgcaccca gcgcacccgc caggacctgg
     1801 gcatcggcag tgagacgctc tcggtgtatc aaaccctgct gccgcgctat tcccagctgc
     1861 tcaaccagta tgaggaaaaa gtggcgcgca cgaacacccg caatctggaa gagaaggcgc
     1921 agagcggtct cgaggggatt cgccgcgagc aggcgacact acaggaatcg ctcgacgcgc
     1981 tcgaacagca gatgctgcag gtggccacaa ccagcacgcg taccgccctg accctgcagc
     2041 gtgaagtgct cgtgacccag cgcgaagcac tcgatgaaag cgggcgactg ttcgagtatc
     2101 agctgcggca gagccgcatc gcgcagcagt tcggtccgat tctacgccct ggcggcctcg
     2161 aagggatgcc ccgcgaacaa atcttgcgca cccggcagga gaccaacgag tggctgcagt
     2221 cactccgcca ggatctcaaa ctggcggaga agcggaaaga gctgtttcaa gccaatcgcc
     2281 agctgctcga gaaagcgatc gcggatctgg aaagccgcac ggggagcgcg gggattgcca
     2341 gccgcgacga gatcaaggct cgccgacagc agctgaagca tcaaactgaa gccggccgca
     2401 aatgcgtaga acaggaacac ctgctcgaaa ctctgattgg cgaggtcgaa cgcgtacaag
     2461 caaccatgac cgaacatcat cttcgcaagc gggaagaaga gctcttcggt cgcagaacgc
     2521 tggccattcg accgaatctg ccgaccctgg tcgaagagac gcttcgccgc ctgcccgcgg
     2581 t
//
LOCUS       DAORHD010000704         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47886_length_2580_cov_4.209505, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000704 DAORHD010000000
VERSION     DAORHD010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47886_length_2580_cov_4.209505"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKO06_14300"
     CDS             complement(<1..574)
                     /locus_tag="PKO06_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV70869.1"
                     /translation="MTDNPFKQNCPHCRRENPVDAMFCTVCGGTLRSETETHGSTLFF
                     GTPAFLLLFGLGVSYDSGGSGISDWGFSLGLALITLYFFSCGALYFPARLLGKTASLV
                     KIWEAGLFGVAQFGLAFILQVPLTEWFASEFSGLNLTQEAQLKESLLLVSFLPLLATG
                     VFLLVRRLPSPTGVVTKPFPWWLNSSLLLGA"
     gene            complement(581..1585)
                     /locus_tag="PKO06_14305"
     CDS             complement(581..1585)
                     /locus_tag="PKO06_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNV70870.1"
                     /translation="MAKPRVLLISTGGTITMVKTPSGDLAPCDSADLLLDRVPEVKTL
                     ADVDLLSLANIDSSNLEPAIWPKLSRELYKRYRDYDGFVITHGTDTLAYTSAALSLMV
                     QELGKPVVITGSQVPLEEIGSDGRANLINSFRVAVADFAEVAVVFGSQIIRGSRAKKT
                     SAFDMQAFTSANAQPLGTIGLSIKFARESIRRSKRKPMLQPFLESSVCLLPVYPGIRP
                     DLVTHVSETHKGLVLEGYGAGNLPTEGPSSLVPAIQTSTGRGVPVVICTQCLVGSTEM
                     ELYRVGRAALQAGAIPAMDMTPEMALVKLMWVLGQTDDLPTIDSMMQKSFVGEVHETV
                     "
     gene            complement(1587..>2580)
                     /locus_tag="PKO06_14310"
     CDS             complement(1587..>2580)
                     /locus_tag="PKO06_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70871.1"
                     /translation="RMEELFEQGTELLRKLQGLSASAKDLIGDPELKASTKAIFKNAE
                     IASAKITEVIESARTKAESIVNHIDGILEKVHTEIDVNRAELRTIVQNMKEFSSNITK
                     ISAENRETVKEMLENVEQVSKKLDRMIDDLNKDRAVTDNIRETVNSLKKASENAKEIT
                     REVKEIVTDKDIRGKIKTGLDDAHKLAQAVDKVFLNIKQTRIDLKYLLRYHKADELFF
                     SDMNVDIWPSDTSFYRIGVEDIGGDPLFNLMLARDAQSKLVKRAGIISSKVGVGIDYR
                     WAEDITYSLDVIDTRKSYARFTTSYMVKPGVNLQLRVDDITKDKEVNFGLEYKF"
BASE COUNT          571 a          691 c          737 g          581 t
ORIGIN      
        1 tcgcaccgag aagcaacgac gaattcagcc accagggaaa cggtttggtg accacgccgg
       61 ttgggctggg caggcgacgc acgagtagaa aaaccccggt tgccagcagg ggaagaaaac
      121 tgacgagcag caggctttcc ttgagctgtg cttcctgggt cagattcaag cctgaaaact
      181 cgctggcgaa ccattcggtc agcggaacct gcagaatgaa ggctaacccg aactgagcga
      241 cgccgaacag accggcttcc cagattttga ccagagaggc ggtttttccg agcagtcgtg
      301 ccggaaaata caacgcacca cacgagaaaa aatacaacgt gatgagcgcc agacccagac
      361 tgaagcccca gtcgctgatt ccgctgccgc cggaatcata cgaaacgcca agaccgaaca
      421 acagcagaaa ggcgggcgtg ccgaagaata gagtcgaccc atgcgtctcg gtttcggaac
      481 gcagggttcc cccgcagacg gtgcagaaca tggcatcgac gggattttca cgtcgacagt
      541 gcggacagtt ctgtttgaag ggattgtcag tcatggctga tcacacggtt tcgtgaacct
      601 cgccgacaaa ggacttctgc atcatggaat cgatggtggg aagatcgtcc gtttgtccca
      661 gcacccacat cagtttcacg agcgccattt caggggtcat atccatagcc ggaatggctc
      721 ctgcctgcaa cgcggctcgc ccgactcggt agagttccat ctctgttgag ccgacgagac
      781 attgcgtgca gatgacgacg ggaaccccgc ggccggtcga agtttgaatg gcaggaacga
      841 gcgatgaggg gccttcagtt ggcagatttc ccgctccata cccctcgagc acgaggccct
      901 tgtgcgtttc cgagacatgc gtgaccagat caggccgaat accgggatac acgggcagca
      961 ggcacactga tgattcgagg aacggctgca gcatcggttt gcgcttcgat cgtctgattg
     1021 attcgcgggc gaatttgatc gaaagtccga ttgtgccgag aggttgagcg ttggccgagg
     1081 taaatgcctg catgtcgaag gcagacgtct ttttggcgcg actcccacga atgatctgtg
     1141 agccgaaaac cacggccacc tcggcaaaat cggcgacggc cacgcgaaaa ctgttgatca
     1201 ggtttgcgcg tccatcagat ccgatctctt cgagtggaac ttgtgaaccg gtgatcacga
     1261 ccggtttccc cagttcctgc accatcaacg acagcgctgc cgaggtgtag gcaagcgtgt
     1321 cggtgccgtg cgtaatcacg aaaccgtcat agtcgcgata gcgtttgtac aactcgcgag
     1381 agagttttgg ccagatggcg ggctcgagat tcgatgaatc gatgttcgcg agtgacagca
     1441 ggtcgacatc agccagagtc ttgacttcgg gaactcggtc aagcagaaga tcggcgctgt
     1501 cgcagggtgc gagatcaccc gagggggtct tgaccatcgt gatagtcccc cccgttgaga
     1561 tcagcagaac ccgcggttta gccatactag aacttgtact ccagtccgaa gttcacttcc
     1621 ttgtccttgg tgatgtcgtc gacacgcagc tgcaggttaa ctccgggctt caccatgtac
     1681 gaggtggtaa agcgggcata ggacttccgg gtgtcgatga catcgaggct gtaggtgata
     1741 tcttctgccc agcggtaatc aatgccgacg ccgacctttg aagagatgat gccagcgcgt
     1801 tttaccagct tgctctgagc gtctcgggcg agcatcaggt taaacagcgg atcaccaccg
     1861 atatcttcga cgccgatgcg gtagaacgag gtgtctgagg gccagatatc gacgttcata
     1921 tcagagaaga acagctcgtc cgccttgtgg tagcgcaaca gatacttcag gtcgatgcgg
     1981 gtctgcttga tgttcaggaa caccttgtcg acggcttgag cgagtttgtg agcgtcatcg
     2041 agcccagtct tgatcttgcc gcgaatatcc ttgtcggtga cgatttcctt cacttcacga
     2101 gtgatttcct tcgcattttc ggaagccttc ttcagcgagt tcacggtttc gcgaatgttg
     2161 tcggtcacgg cgcgatcctt gttcagatca tcgatcatgc gatcgagttt cttggagacc
     2221 tgttcaacgt tctcaagcat ttccttgacc gtttcgcggt tctcggcaga gatcttggtg
     2281 atattgctgc tgaactcttt catgttctgc acgatggtac gcagttcggc acgattcacg
     2341 tcgatctcgg tatgcacttt ttcgagaatg ccatcgatgt ggttcacgat actctcggcc
     2401 ttggtccgag ctgactcgat gacttccgtg atcttggcac tggcaatttc cgcgttcttg
     2461 aagattgcct tcgtgctggc cttcaactcg ggatcaccaa tgagatcctt ggcggaggcg
     2521 ctgagaccct gaagcttgcg caacaactca gttccctgtt cgaagagttc ttccatgcgg
//
LOCUS       DAORHD010000705         2579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47919_length_2579_cov_2.675911, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000705 DAORHD010000000
VERSION     DAORHD010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47919_length_2579_cov_2.675911"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2180)
                     /locus_tag="PKO06_14315"
     CDS             complement(<1..2180)
                     /locus_tag="PKO06_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70872.1"
                     /translation="MIAGLLLAFPVLLLLPPILSLLATREAVPLRKTMRTVQEEVSLV
                     SKLTEPKALAATLLRSWSRDLQASITRDQLSDESVTAFRQAIQPLFLKLFPHSACAVF
                     RGDPRLERYQNLANHINRVIANTLHPWWIPYFMCVFSPERLSHDLFWIAGDVPGFSEQ
                     VVRDIGACLKGYARNVFDGFLHHHLSRYLDHSGFGYPKRLWQESLGVFVESVVGDERR
                     YFCWYPLYSASGAHRFSWPDSLPESEAERSIVAQRELRGGLLFFLSPEDLDERSYKPA
                     LQRLLSDRGSRLLFPSDSLTQSASASHLPASPPADRSTRSHTVAGELMLQSPEPYILS
                     RTIPDAPEPATWWRVIVPLSTFIWLCLGISFVVLPLRKREAPALSLLGQMAVVFFLIL
                     VPALCLALITLERSSAEHQAQVETRCRKDLQRLITTIDEGQRLYLGWIVNETETLTRE
                     PSIQQLIVRADSSKNQQLELIGDKLIQDLYQRGLQRGLWLSRVFLLGLGGTSNCFSNR
                     TDIAPIVSRTLQQITFPALKRLTSDRASGAMRDQASDLAIGFGVEEMRLLISTIFPSD
                     LLADMANGPRSLSNFVFADEQFLFFKKLLTIDTVPRGILQLQLRTETVDFHNLTSWKN
                     LTLAPSQRDIPIFSFWSGFKSTPFVYSPVPYLRFKLDTDGTLEMDVTESLQAMSVGEL
                     AAVAVQTDGPVMQELGGGTKRQLLLAQPGQAMNDSVLSAMTPVGL"
BASE COUNT          624 a          658 c          786 g          511 t
ORIGIN      
        1 agacccaccg gagtcatggc cgagagaacg gaatcgttca ttgcctgccc aggttgtgcc
       61 aacaggagtt ggcgcttcgt gccaccaccg agctcctgca tgaccggccc gtccgtttgc
      121 accgccactg cagccagctc accgacgctc atggcctgca gcgattcggt cacgtccatc
      181 tcaagagttc catcggtgtc aagcttgaat ctcagatacg gaacgggtga ataaacaaat
      241 ggggtgcttt taaatcctga ccaaaagctg aaaatcggaa tgtctcgctg ggacggagcc
      301 aacgtgagat tcttccagga ggtcaggttg tgaaaatcga ctgtctctgt tcgcagctgc
      361 agttgcagga tgccacgtgg aacagtatcg atggtgagaa gctttttgaa aaagagaaac
      421 tgctcatcag caaacacgaa attggaaagc gatctcggcc cgttggccat gtcggcaaga
      481 aggtcactgg gaaaaatggt gctgatcaac agtcgcatct cttcgacgcc gaacccgatg
      541 gccagatcgg aagcctgatc acgcatggct cccgaggcac ggtcactggt caaacgtttg
      601 agggcgggaa aagtgatctg ctgcagcgtg cggctgacga tgggggcgat atcggttcga
      661 ttcgagaagc aatttgaggt gccgcccagc ccgagcagaa agacgcgact gagccaaaga
      721 ccgcgttgga gacctcgttg atacagatcc tgaatcaact tgtcgccaat gagctcgagt
      781 tgctggttct tcgaactgtc agcccgtact atcagctgct gaatggacgg ttctcgggtc
      841 agagtttccg tctcgttcac gatccaaccc aggtagaggc gttgtccttc atcaatggtg
      901 gtgatcagac gctgcaggtc tttccggcat ctggtttcca cctgagcctg atgttcggcc
      961 gaggaacgtt cgagggtgat gagcgcgagg cagagagccg ggaccagaat cagaaagaac
     1021 acgaccgcca tttgtccgag caatgaaaga gcgggagcct cgcgctttcg cagtgggaga
     1081 acgacgaaag agatacccag gcacagccag atgaaggtcg aaagtggcac aatcacgcgc
     1141 caccacgtgg caggttccgg agcgtctgga attgtccggc tcaaaatgta cggttctggt
     1201 gactgcaaca tcagttcgcc ggccaccgta tgacttcgcg tcgaacggtc tgctggcgga
     1261 gatgccggga ggtgagaagc ggatgcactc tgggtcagac tatctgaggg aaacaacagg
     1321 cggctgcctc tatcggagag gagccgttga agtgccggct tgtacgaacg ttcatcgaga
     1381 tcttcgggtg agaggaaaaa cagcaacccg ccgcgaagtt cgcgttgagc cacgatcgac
     1441 cgttcagcct cggattcggg taacgagtcg ggccaggaga accggtgtgc cccggaagcg
     1501 gaatagagcg ggtaccagca gaaataacgt cgttcgtctc cgacgacgga ttcaacgaag
     1561 actcccaggc tttcctgcca gagacgcttc ggatatccga agccgctgtg atcaagatac
     1621 cgggacaaat ggtggtgcag aaaaccgtca aagacgttcc tggcataccc cttcagacag
     1681 gcaccaatgt ctcgaacaac ctgttccgag aatcccggaa cgtcgccggc aatccaaaac
     1741 aggtcatgcg acagacgctc aggcgagaaa acgcacatga agtatgggat ccaccatgga
     1801 tggagcgtgt tggcgatgac acgattgatg tgattggcga gattctgata ccgctcgaga
     1861 cgaggatctc cacggaacac ggcgcaggcc gaatggggaa agagtttcag aaagaggggt
     1921 tgaatggctt gtcgaaaggc ggtgacggac tcatcgctca gctgatcacg ggtgatcgac
     1981 gcttggagat cgcgactcca tgagcggagt aaggtcgccg cgagcgcttt gggttccgtg
     2041 agcttcgaaa ccaggctgac ttcttcctga acggttcgca tggtcttgcg caaaggcact
     2101 gcttctctgg tggccagaag tgacagaatc ggcggaagaa gcagcaatac cgggaaagcg
     2161 aggagcagac cggcgatcaa gagagaccgg gtgggtctgg gcccatgagc attttctgca
     2221 gcgcatgcgg gtgagggtgg gctgggttga gtcgaaaggg ctcgaagcga caaatcactc
     2281 tccccctgcg tgtctaatga ggtcttgatc tgagtcggat actggcagtc gctgtcggga
     2341 atctcaattg ccatcaccga agaacgttca aaagagcggt cttctgaagt tggtgactcg
     2401 gacggtcgat aattcacggc agagagttcc cacgcgttcg ggaaatctgg atcttcgagg
     2461 ggaatgaaag tgaatccttc tccagcatgc tctcgcaccc gatctgagca gatgatgagt
     2521 cgggcctttc cctttttgga agcaccctca agatcggcgg cgtacttaaa gggctcgcc
//
LOCUS       DAORHD010000706         2578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_47936_length_2578_cov_3.662703, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000706 DAORHD010000000
VERSION     DAORHD010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47936_length_2578_cov_3.662703"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PKO06_14320"
     CDS             <1..2070
                     /locus_tag="PKO06_14320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV70873.1"
                     /translation="YLGKAVCISGAAVGSLCVVFQRDVVPAAEDLRVISIIASAIGTE
                     ESRRQAEIVLREREEKDRILFEVESDALFLIDNEDGKLIEANPAAVQLYGYSHEELLR
                     MRNIDLSAEPQDTRHVTRSHQPDPGTVVNIPLRWHKRKDGTIFPVEITGRFFLQNGRG
                     VHIAAIRDISHRQASEQALKDSEQNYRAIFDSTNEAIFIDEAATGRVIDVNASMLKMY
                     GYDSKEEVLAGDLGDLSANEPPFTQKEASANVRKAIEVGPQIFEWLARKKSGERFWVE
                     VSLRSVVIGGKQRVLAVVRDISERKRLEEQLIQSQKMESVGRLAGGVAHDFNNMLMVI
                     LGRCDLLLARLPADSPLRRDLLDIQQVATRSASLTQQLLAFARKQIIMPKVIQLNDVV
                     EGMLRMLQRLIGEDIRLRWIPGPNLWNVKLDPAQVDQVLANLLVNARDAISTNGQITI
                     ETRNAPITQADRSAHPDLTPGEYVVLVVNDNGVGMTPDVIQKIFDPFYTTKDVGKGTG
                     LGLATVYGIVQQNRGGIEVVSEAGKGSTFCLYFPRDVSHESKNLSVTDTPVLPTGTET
                     IMLVEDDHTILALVQQLLEQLGYHVISSESPVAALAKLDAESGPVDLLLTDVIMPEMN
                     GRQLFENMRKRRPAVRGLYMSGYTADVIADRGLLDESCNFIQKPFQAAEIAQKIRAIL
                     DRRGEQS"
     gene            2067..>2578
                     /locus_tag="PKO06_14325"
     CDS             2067..>2578
                     /locus_tag="PKO06_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70874.1"
                     /translation="MTIARKFSLGFLCAALLPLLVMGLSGLTIVGDFRDRLHQNRQQA
                     VVDAQKRVLNDAEDKALLIRQFLQSYQKDIDTLRRLHESYLQEAHGCESSYSRMYQDK
                     PTAGLPACGYVHPEFQSFGNWDNRLYAGSVWITKPIVRRITTDQTFRDEIGRQVQATI
                     HMIPNLQAFE"
BASE COUNT          577 a          682 c          766 g          553 t
ORIGIN      
        1 tacctgggca aagccgtgtg tatcagtggt gcagcggtag ggtcgctgtg cgtcgtgttt
       61 cagcgcgacg tggttccggc tgcagaagat ctgcgtgtca tcagtattat cgcgtcggcc
      121 attggtacag aagaatcacg acgtcaggcg gaaatagttc tgcgtgagcg tgaagagaag
      181 gatcgcatac tgttcgaggt cgagtccgat gccttgtttc tcatcgacaa tgaagacggg
      241 aaactgatcg aagccaatcc ggctgcggtg cagctgtatg gatattcaca cgaagaactg
      301 cttcgtatgc gcaacatcga tctctctgcc gaaccgcagg acacccggca tgtcactcgc
      361 tctcatcagc ctgatccggg aaccgtggtc aatatcccgc tgcgttggca taaaaggaaa
      421 gacggcacca tctttcccgt ggagattacc gggagattct ttctgcagaa tggtcgtggg
      481 gtccacattg ccgccatccg cgatatctcg catcgtcagg cttctgagca ggcgttgaaa
      541 gacagcgaac agaactaccg ggcgatcttc gactcgacga acgaagcgat attcatcgat
      601 gaggcggcga cggggcgcgt gatcgacgtc aacgcttcga tgctgaaaat gtatgggtac
      661 gactcgaaag aagaagtgct cgcgggagac cttggggacc tgagtgcaaa cgagccccca
      721 ttcacgcaga aagaagccag cgccaacgtc agaaaggcga ttgaagtggg gccgcagatc
      781 ttcgagtggc tggcgcgcaa aaagagcggc gagcgattct gggtcgaagt gtcgttgcgg
      841 agcgtggtca tcggtggcaa acagcgagtt ctggctgttg tacgagatat cagcgaacga
      901 aaaaggcttg aagagcagtt gatccagtca cagaaaatgg aatctgtcgg tcggctcgcc
      961 ggcggcgtgg cgcacgattt caacaacatg ctgatggtga ttctgggccg ctgcgacctg
     1021 cttttggccc ggctgcccgc ggattccccc ctgcgaagag atctgctcga cattcaacag
     1081 gtggcgacgc gatcggcttc cttgacccag caattgctgg ccttcgcccg caagcagatc
     1141 atcatgccga aagtcattca actcaacgat gtggtcgaag gcatgctgcg tatgctgcag
     1201 cgcctgatcg gcgaggatat tcggttgcgc tggattcccg gccccaatct ctggaatgtc
     1261 aaacttgacc cggcgcaggt cgatcaggtg ctggcgaacc tgctggtcaa tgcgcgtgac
     1321 gccatttcca cgaacgggca gatcaccatt gaaacccgaa atgcgccgat cacccaggcc
     1381 gatcgcagtg cgcaccctga cctcacgccg ggtgagtatg tcgtgctcgt ggtgaatgat
     1441 aacggggttg gcatgacacc ggacgtgatc cagaaaattt tcgatccctt ctataccacc
     1501 aaagacgtgg ggaaaggcac ggggctcggc ttggcaaccg tctacggcat cgtgcagcag
     1561 aatcgtggtg gcatcgaagt ggtcagtgaa gcaggcaagg gttcgacgtt ctgcctctat
     1621 tttccgagag acgtatctca cgagtcgaaa aacctatcgg tgacagatac tcctgtgttg
     1681 ccaaccggta ctgaaaccat catgctcgtc gaggatgatc acaccattct tgctctggtg
     1741 caacagctgc tcgagcagct tgggtatcac gtcatctcca gtgaatcccc cgttgccgct
     1801 ctggcaaaac tcgatgccga gtcgggcccc gtcgatctgc tgcttaccga tgtcatcatg
     1861 cccgaaatga acgggcgaca acttttcgag aacatgcgga agcggcggcc ggccgtgcgt
     1921 ggtttgtaca tgtcgggcta taccgcggac gtgattgctg atcgaggtct gctcgacgaa
     1981 tcctgcaact tcattcagaa accgtttcag gcagccgaga ttgcgcagaa aatccgcgcg
     2041 attctcgatc ggcggggaga gcaatcatga ccatcgcgcg caaattttcg cttgggtttc
     2101 tgtgcgcggc attgctcccg ctcttggtca tggggctttc cgggctgacg attgtcggtg
     2161 actttcgtga ccggctgcac cagaatcgcc agcaggcagt ggttgatgcc cagaagcgag
     2221 tcctgaatga tgccgaagac aaagcgttgc tgattcgcca atttctgcaa tcgtatcaga
     2281 aagatatcga tacgctgcgt cggttgcatg aatcgtatct gcaggaagcg catggatgtg
     2341 agtcgtcgta ttcgcgaatg taccaggata aaccgacggc cgggcttccg gcatgcggct
     2401 atgtgcaccc ggagtttcag agtttcggaa actgggacaa tcgcctgtat gccggcagtg
     2461 tctggatcac caagccgatc gtgagacgca tcaccacgga ccagacgttt cgcgacgaga
     2521 ttgggcgcca ggtgcaggcg acgattcaca tgatcccgaa tcttcaggct tttgagga
//
LOCUS       DAORHD010000707         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48073_length_2574_cov_3.479158, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000707 DAORHD010000000
VERSION     DAORHD010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48073_length_2574_cov_3.479158"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(490..645)
                     /locus_tag="PKO06_14330"
     CDS             complement(490..645)
                     /locus_tag="PKO06_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70875.1"
                     /translation="MPALPRAQLIVSFPLNRLPQRLVAKDAQGFKVERIRALDDYEDE
                     QALREMP"
     gene            complement(618..1889)
                     /locus_tag="PKO06_14335"
     CDS             complement(618..1889)
                     /locus_tag="PKO06_14335"
                     /inference="COORDINATES: protein motif:HMM:NF007640.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WcaI family glycosyltransferase"
                     /protein_id="HNV70876.1"
                     /translation="MQIVIHALNFHPEEVGCARYNTELALWLARRGHRVKVVTTPPYY
                     PWWQVQPPYLWHQYRHETLDNLDIYRCPLWVPRQPTGSARIWHLLSFGVSSLPYAFVP
                     PWQGSDPQFKPDLVFTVQPTLFGAFSALLMARRLQAKAWLHVQDFELEAALATRLIGL
                     KKGGVLPIRVANRLERFFYTRFHRLSVISRTMAQRLADKGLPEEKIVYLPNWVDTNVI
                     RPLSRDSIMRHELGLSISQRVVLYSGNMGRKQGLDIILRIAPSLARSHPDLVFLLVGD
                     GSGRADLKADVRRRGLTNVRFAPLQPVAKLNDLLNLADLHLVLLTHGVRDSVLPSKLT
                     TILAAGGVAIASAEPGTELMQLISRHQCARAVPPEDEAALRTAITSLLADSDRCRAIS
                     AAARRLAESQFSIDSVLPRFETQCLRYLGLS"
     gene            1982..2221
                     /locus_tag="PKO06_14340"
     CDS             1982..2221
                     /locus_tag="PKO06_14340"
                     /inference="COORDINATES: protein motif:HMM:NF015807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HNV70877.1"
                     /translation="MPSITVKNIPEALHRQLRQRAKEHDRSINGEILFILRSVCRKTP
                     QRLKGLEDRITALRNSMSGPVSEKLIDEAIETGRR"
     gene            2218..>2574
                     /locus_tag="PKO06_14345"
     CDS             2218..>2574
                     /locus_tag="PKO06_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV70878.1"
                     /translation="MIVVDTNILLYLTIPTVHSVSVQLAWKKDPEWASPVLWRSEFKN
                     AILVLIRKGVVSSEYAQKALERAAEVVDVHEYLPDDKLVMDLALRTTCSGYDCEFAAL
                     ARELETFLLTYDKKVLA"
BASE COUNT          577 a          689 c          818 g          490 t
ORIGIN      
        1 cgttgtcgaa acgtcgcggc gacaactgca gtcaaggccc ccgagaaagc cgcggccagg
       61 accgcctcga acaacacaac gaaagcatct gttcgagccg ctgtgcgtcc ggcaaaaaag
      121 gcctccccac gccggtaacc gcgcctcact tcttcgtgcc acaacgatcg caagaatgaa
      181 cgagcagatt gcggaaccgg accctggaag caaaggttcg gtgttgtgcg tcggtgatga
      241 ttccggcttc ggaaaggatg aggaaggtgt ctgcatcggt ttctgggtgg cgtagttcga
      301 ggtcgcaggc gagatgcttg gcgatatcga tcatgacatc gatggccagt tcgagattgc
      361 gctcaagaaa acgcttgaga acgaggttac agaaaacgtg agcgtcgttt tcaggaagat
      421 acctgacgta tctccgccac gagttgagcc agaagagcgt tcctgcgctc gagaagcggc
      481 ttatcgacct tagggcatct cacgcaaggc ctgttcgtct tcgtagtcat cgagagcgcg
      541 aatacgttcg actttgaatc cctgcgcgtc tttggcaacc aggcgttgcg gaagacggtt
      601 gagcgggaac gagacgatca gctgagcccg aggtagcgca ggcactgggt ttcgaagcgg
      661 ggaagcacgg agtcgatact gaactgtgat tcagccaggc gacgggcggc ggcgctgatg
      721 gcgcggcatc gatcggagtc ggcgagcaga ctggtgatcg cggttcgcaa ggctgcttcg
      781 tcttcgggcg gcaccgcgcg ggcgcattgg tgccgggaaa tgagctgcat cagctcggta
      841 ccaggttcgg ctgaggcgat ggccacacca cccgccgcca gaatcgtggt cagcttcgag
      901 ggcagcacgc tgtcgcggac cccatgcgtg agcaagacca ggtgcagatc ggcgaggttg
      961 agcaggtcgt tcagcttcgc caccggctgc agcggagcaa agcgcacgtt ggtcagaccg
     1021 cgccgtcgca cgtcggcttt gagatcggcg cggccactgc cgtcaccgac gagcagaaag
     1081 acgagatcgg ggtgtgagcg cgcgagcgac ggagcaatgc ggagaatgat gtcgagcccc
     1141 tgcttgcgac ccatgttgcc agaatacaga acgactcgtt gcgagattga aagtccgagt
     1201 tcgtgacgca taatactgtc tctggataat ggtctgatga cattcgtatc aacccagttc
     1261 ggcagataga cgatcttttc ttcgggtagg cccttgtcgg cgaggcgctg ggccatggtg
     1321 cgggagatga cgctgagccg gtgaaaccgg gtgtagaaaa aacgctcgag ccggttcgcc
     1381 acgcggatcg gcagcacccc gcctttcttg agcccgatca gccgcgtcgc cagtgccgct
     1441 tcgagttcga aatcctggac atgcagccac gccttggcct gcaggcgccg ggccatgagc
     1501 agagccgaga aggcgccaaa gagcgtcggc tgaaccgtaa agaccagatc gggcttgaac
     1561 tgcgggtctg aaccctgcca gggtgggaca aacgcatacg gcagagacga caccccgaac
     1621 gacagcagat gccagatgcg cgccgacccg gtcggttgcc gcggtaccca gagcggacag
     1681 cggtagatgt ccaggttgtc gagtgtctca tgccggtatt gatgccagag atagggaggc
     1741 tgcacctgcc accacggata gtagggtggg gtggtgacga ccttcacccg gtgcccacgc
     1801 cgcgccagcc acagggccag ttcggtgtta tagcgcgcgc agccgacttc ctcggggtga
     1861 aagttcagcg cgtgaatgac aatctgcatc gcatcaattt accgcaaatt cgggatcttg
     1921 tctcaagacg cgaatcagga ttatactcaa agcaaccatt atggttgctc aggaggcgag
     1981 tatgcccagt atcacggtga aaaacattcc ggaagctttg cacagacagc tgagacaaag
     2041 agccaaagag catgatcgca gtatcaacgg agagattctt ttcattctac gctcggtgtg
     2101 caggaagaca ccgcaacgat tgaaagggct ggaagaccgc attaccgctc tccggaacag
     2161 tatgtcgggg ccggtatcgg aaaaactcat tgacgaggcg attgagacgg ggcgtcgatg
     2221 atcgtcgtcg atacgaacat tctgctctat ctgacgattc cgacggttca ctccgtttcc
     2281 gttcagcttg cctggaaaaa ggacccggag tgggccagcc cggtgctctg gcgttcggag
     2341 ttcaagaacg cgattcttgt gctgattcgc aagggagtcg tgagttccga atatgcccag
     2401 aaagctctgg aaagagcggc agaggttgtc gacgttcacg aatatctccc cgacgataaa
     2461 ctggtcatgg atcttgctct gcggacgacg tgctcggggt atgactgcga attcgcggcg
     2521 ttggcgcgtg aattggagac gttcctactc acgtatgata aaaaggtgct ggcc
//
LOCUS       DAORHD010000708         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48095_length_2573_cov_4.287530, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000708 DAORHD010000000
VERSION     DAORHD010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48095_length_2573_cov_4.287530"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..558)
                     /locus_tag="PKO06_14350"
     CDS             complement(127..558)
                     /locus_tag="PKO06_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV70879.1"
                     /translation="MNGPSAPTQSRTNATLPRGTERLLLIDDEPELTTCCKEFLSRLG
                     YHVSTRNNPVEAVQLVKQDPQAFDAVITDMTMPKMTGFKVAKELLLIRPDLPIILCSG
                     DAEGQGEKNLTEQGFRGFLQKPFIMREMAETLRAILDQKTG"
     gene            521..1201
                     /locus_tag="PKO06_14355"
     CDS             521..1201
                     /locus_tag="PKO06_14355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV70880.1"
                     /translation="MVRLCVGADGPFMAGRKTRIEEIPQALSQMEEGVKRISTIISAI
                     KSFSHPSKGARGPADLNRGIEDTVTISRNEWKYAADLVTMLSPNLPAVPCFLDQINQV
                     ILNMIINATHAIEDSIKAGRYKKGKIIISTGIREQQALITIRDDGAGIPSECVPRIFD
                     PFFTTKPLGKGTGQGLSIAHDIIVQRHGGRIEVDSESGVGTTFRLFLPLKILEPITST
                     STSQGEPQ"
     gene            1198..2424
                     /locus_tag="PKO06_14360"
     CDS             1198..2424
                     /locus_tag="PKO06_14360"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV70881.1"
                     /translation="MNLPGVLFVDDEPHIIQSLRRALHSSSREWRLSFAQSGQEALAL
                     MRRGLAQGEPIDLLVSDMRMPEMNGAELLKQVRQEFPHTIRFVLSGHSDQDMILQTVS
                     SAHQFLSKPCEVDLLKQCIRRAFGLRSQLRTPEVLRLASGVRSMPTPDRLFHEICDAL
                     QQPRVSLEEIGEMVAQDPTVSSRILHLANSAFFGLAREVVQVPEAVVFLGIQIVKAVI
                     LSASIFESQSQDTPKGKFVQSVQEHSLLVAGLSRHLCLSLEGSSRLAEECFIAGLLHD
                     LGKLLLLQHSRYFPELPLMARSQNKPLHAVESLALGCHHGSLGGYLLGLWGLPDNVVE
                     AVLWHHHPEQAIGTRLRPLVTVSLANAFVSSAEPERHTREELSEEFLKTVNLHQRLDH
                     LRTIARQFLSEARGTP"
BASE COUNT          548 a          771 c          685 g          569 t
ORIGIN      
        1 gagtcggctc cgtggtcaca ctcttcgggc tttccactcg attccccatc ttatcagaca
       61 gggtaacaga aaccctcgtg cgaggaaacg gcggcctcga tgcgggtatc tgtttccgga
      121 ccaggatcag ccggttttct gatccagaat ggcgcgcaga gtctcagcca tttcccgcat
      181 aataaagggc ttctgcagga atccacggaa tccctgctcc gtcaggttct tttcgccctg
      241 accttctgca tctcccgagc agaggatgat cggcagatcc gggcgaatca gcaacagttc
      301 cttcgcgacc ttgaaaccgg tcatctttgg catcgtcatg tcggtaatga cggcatcgaa
      361 ggcctgcgga tcctgtttca ccagctgaac ggcctcaacc ggattgtttc gcgtgctcac
      421 gtggtaacca aggcgagaca agaattcttt acagcatgtc gtcaactcgg gttcgtcatc
      481 gatcagaagc aggcgctccg ttcctcgagg gagagtggcg ttggtacggc tttgcgtcgg
      541 cgctgacgga ccattcatgg cgggcagaaa aacccggatc gaagaaattc cgcaggcgct
      601 gtcccagatg gaagagggtg taaaacgcat cagtactatc atttcagcta tcaagagttt
      661 ttctcacccg agcaagggtg ctcgcggacc cgctgacctg aatcgaggca tcgaagacac
      721 cgtgacgatc agtcgcaacg aatggaagta tgccgctgac ctcgtgacga tgctctcgcc
      781 taacctgccg gccgtcccct gctttctcga ccagatcaat caggtcattt tgaacatgat
      841 catcaatgcg acgcacgcca ttgaagattc catcaaagcc ggcaggtaca aaaagggaaa
      901 gatcatcatc agcacgggca ttcgcgaaca acaggcattg atcacgattc gcgacgatgg
      961 agcgggaatt ccctctgagt gcgtgccacg catcttcgac cccttcttta ccaccaaacc
     1021 gctgggaaaa gggaccggcc agggcctgtc tattgcgcac gatatcatcg tgcagcgcca
     1081 tggaggtcgc atcgaagtgg acagcgagtc aggggtcggg acaacatttc gtctctttct
     1141 gcctctcaaa attctcgagc cgatcacctc aacttcgacc agtcagggag aaccccaatg
     1201 aacctacccg gcgtactgtt cgtcgatgat gaacctcaca tcattcagag tcttcgtcgc
     1261 gccctgcatt cttcatcgcg tgaatggcga ctgtctttcg ctcagagcgg tcaggaagct
     1321 ctcgcgttga tgcggcgagg tcttgcccag ggggaaccga tcgatctgct ggtcagcgat
     1381 atgcgcatgc ccgaaatgaa tggggccgaa cttctgaagc aggtccgaca ggagttcccg
     1441 cacacgatcc gcttcgtact ttccggtcac tcggaccagg acatgatttt gcagaccgtc
     1501 agctcggcgc atcagtttct ctcgaaaccc tgcgaagtcg atctcctcaa acagtgcatc
     1561 agacgtgcat tcggactccg tagtcaactg cgcactcccg aagtgttacg actcgcttcc
     1621 ggtgttcgct ccatgccgac acccgaccga ctgttccacg aaatctgcga cgcgctgcag
     1681 cagccgcgtg tctcgctcga agagatcggt gagatggtgg cgcaggatcc gaccgtatct
     1741 tcccgtatcc tccatttggc gaactctgcg ttcttcggcc tggctcgcga agtcgtccag
     1801 gttcctgagg ccgtcgtatt cctcggcatt cagatcgtca aggccgtcat cctgtccgca
     1861 tcaattttcg agagtcagtc tcaggatacc cccaagggga agttcgttca gtcggttcag
     1921 gaacacagtc tgctggtcgc gggtctctct cgccatctct gcctcagcct ggagggatcg
     1981 agccgactgg cggaagagtg cttcattgca ggattgttac acgatctcgg aaaactgctt
     2041 ctattacagc attcgcggta ctttcccgaa ctgccactga tggcccgcag ccagaacaaa
     2101 cccctgcatg ccgtagagtc tctcgcactc ggttgccatc acggctcact cggcggttat
     2161 ctgctcgggc tctggggtct gccggacaat gtcgtcgaag ccgtgctctg gcatcatcac
     2221 ccggagcagg cgatcggtac tcgtcttcgc ccactcgtca ccgtctctct ggcgaatgcg
     2281 ttcgtctctt ccgcagaacc tgaacgacac acgcgcgaag agttgagtga ggagtttctg
     2341 aaaaccgtga accttcacca gcgactcgac catctgcgca ccatcgcccg ccaatttctc
     2401 tcggaagcga ggggaacacc atgaacagac gggtgctgct cgtcgacgac gaacaaaacg
     2461 tcctgctcgg ctatactcgc atattgcgaa aacatgccga tgtcgttacg gcatccggag
     2521 cggtgaccgg actgcaggcg ctcgtacacc aaggtccatt tgccgccgtg gtt
//
LOCUS       DAORHD010000709         2573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48103_length_2573_cov_3.239873, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000709 DAORHD010000000
VERSION     DAORHD010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48103_length_2573_cov_3.239873"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKO06_14365"
     CDS             <1..457
                     /locus_tag="PKO06_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNV70882.1"
                     /translation="PAGNNPDRITGAAALPEVITVGALDQWKRRALFSGQGPAVVDAV
                     PIPKPDLAEPGVAIFGPSYDSVPFRYGSGTLQAAAHFAGIYALLKEVRPQESPDSFLY
                     AMATTTADLGTPGVDMETGMGLPDPNAALYFLENPPTEEPVPTFYINKP"
     gene            complement(542..2515)
                     /locus_tag="PKO06_14370"
     CDS             complement(542..2515)
                     /locus_tag="PKO06_14370"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV70883.1"
                     /translation="MASIKQLFLTIANEKDSELEACEHLDAFARKNGFAPEVIDEMRL
                     AFIEAIINAKEHAPKNTPEQNADVYCEVSFDGDALTIKVRDYGQGFDPTVVEKPDIKK
                     KLKSAHKRGWGLMLMEKLMDGMDISSFPPSGTLITMVKKRLRLEPPESPDVAKEKKRI
                     ERLKYIYGSFIDLSSFLCQNRDLESGLRSMLRILLGTMGVSRGAIYSYDVADGGFTAL
                     VDIKVKSREKAPFLAVNERDMQQLMAQETQELAKALGNCCPDFATHFGKEEIEIVSLL
                     RTDSEVLGMLMLGPRFKSGKEEPEPFDPELLTTLSRNISSAINTFHLMDQLRVANNEL
                     DARFREMENIREASVKISSILEIENLPRATEKIFAEIMRIGKFSLALFEPGENRFKIC
                     LTGRDLPEILDLWSSPVARFVIQKKHALFVPNVTSETRFQFPRAGNYKTKSFISIPIV
                     VQEEVLGLVNLTDKTDGTDLDEHDYQLAQLLCSQLNVAIRNANLYKLGFTDSLTHLYA
                     TNYFRIRMAQEIARFRRVKSHLSLIMLDVDHFQNVIDAHDLSVGDGLLARVSNAIKHI
                     LRFNDIPCRYGRERLAIILPDTDGKGASRVAEKIRSGIENTKVVVNGQEVKATVSMGV
                     SEFTVQMSLDQMIELGEKRLAEARDKGGNRVIG"
BASE COUNT          560 a          717 c          683 g          613 t
ORIGIN      
        1 tccggccggg aacaaccccg atcgtatcac cggtgctgca gctctccccg aagtgatcac
       61 cgtcggggca cttgaccaat ggaaacgacg ggccctgttt tcaggacagg gtccggcagt
      121 tgtcgatgcc gtgccgattc ccaagcccga tctggctgag cccggagtag ccatcttcgg
      181 ccccagttat gattccgttc ccttccgcta tggatcagga accctgcaag ccgccgctca
      241 tttcgcggga atctatgccc tgttgaaaga agttcgccca caggaatctc ctgacagctt
      301 tctgtatgcc atggccacaa ccacggccga tctgggcact cctggtgtcg atatggaaac
      361 cggaatgggt ctgcctgacc cgaacgccgc cctgtatttt ctggagaatc ctcccacgga
      421 agaaccggtt ccgacgttct acatcaacaa accctgacca aaaactgccg ccattatcgg
      481 cctaaaagca caccacccgg cctaagccgg gtggtgtgct tttaggccat ctttgagagc
      541 ttcatccgat gacgcggttt ccgcctttgt cacgagcctc agcaagacgt ttttcaccca
      601 gttcgatcat ctgatcgaga ctcatctgca cggtgaactc cgaaacgccc atactgacgg
      661 tcgcctttac ttcctgtccg ttcacgacca ccttcgtgtt ttcaatgccc gagcggattt
      721 tttcggcaac gcgcgacgcc cctttcccat ctgtgtcggg gagaatgatc gccagacgtt
      781 cgcggccgta ccgacaggga atgtcgttaa aacgcagaat gtgtttaatg gcattagaaa
      841 ccctcgccag aagtccatcg ccaacagaaa gatcatgtgc atcgatgacg ttctgaaagt
      901 gatcaacatc tagcatgatc aatgataaat gcgatttcac acgcctgaag cgagctatct
      961 cttgagccat gcgaatgcgg aaataattcg tggcatacag atgggtcagg ctgtcggtaa
     1021 acccgagttt gtacaagttc gcattgcgaa ttgcgacgtt caactggctg cagagcagct
     1081 gcgccagttg atagtcgtgc tcatccagat ctgtgccgtc ggttttgtcg gtcagattta
     1141 ccaaaccgag aacctcttcc tgcacgacaa tcggaatgga aataaaggat ttcgtcttgt
     1201 agttacccgc gcgaggaaac tgaaaacgtg tttcgctggt gacgttcggc acaaataagg
     1261 cgtgcttctt ctggatgacg aacctcgcga ccggcgaaga ccacagatca agaatctccg
     1321 gcagatcgcg accggtcagg cagatcttga aacgattttc gccaggttcg aacagggcca
     1381 gcgagaattt tccgatgcgc atgatctcgg caaagatctt ttcggtggca cgtggcaggt
     1441 tttcgatctc gaggatggat gagattttga cgctggcttc acgaatgttt tccatttctc
     1501 tgaaccgcgc atcgagttca ttgttggcca cgcgcagttg gtccatgagg tggaaggtgt
     1561 tgatcgccga tgagatgttg cgcgaaaggg tcgtcaacag ttcgggatcg aacggctctg
     1621 gctcttcttt cccagacttg aagcggggcc ccagcatgag catgccgagc acttcgctgt
     1681 cggtgcgcaa caggctgacg atctcgattt cttccttgcc aaagtgtgtg gcgaaatcgg
     1741 ggcagcagtt gccaagcgct ttggcgagct cctgggtttc ctgggccatg agctgctgca
     1801 tgtcgcgttc gttcaccgcc agaaacggcg ccttctcgcg tgacttcacc ttgatgtcga
     1861 ccaacgccgt aaagccaccg tcggcgacgt catacgagta gatcgctcca cgggaaactc
     1921 ccatggtgcc cagcagaatg cgcagcatcg agcgcaaccc gctctcaaga tcgcgattct
     1981 gacagagaaa tgaactcaga tcaataaaac tgccgtaaat gtacttcagt ctctcgatgc
     2041 gttttttctc tttggcgaca tcgggtgatt cgggcggctc aaggcgcagg cgctttttca
     2101 ccatggtgat cagcgtgccg gaaggcggga acgaggagat atccatgccg tccatgagct
     2161 tttccatgag catgaggccc cagccgcgtt tgtgggcaga tttcagtttc ttcttgatgt
     2221 caggcttttc gacgaccgtg gggtcaaaac cctggccgta atcgcgcact ttgatggtga
     2281 gcgcgtcccc gtcgaaactc acttcgcaat agacatccgc gttctgttct ggcgtgttct
     2341 tgggcgcgtg ttccttggcg ttgatgatgg cttcgatgaa ggccaggcgc atttcatcaa
     2401 tgacttccgg ggcaaagcca ttcttcctgg caaaggcatc caggtgttca caagcctcga
     2461 gttcggaatc cttctcgttg gcaatcgtca gaaacagttg tttaatggaa gccacgtggc
     2521 tgctccttcc ggtcagaccg gcaatcttcg atcaacgcca gaacaaccgc cat
//
LOCUS       DAORHD010000710         2571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48146_length_2571_cov_3.896661, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000710 DAORHD010000000
VERSION     DAORHD010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48146_length_2571_cov_3.896661"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..1091
                     /locus_tag="PKO06_14375"
     CDS             288..1091
                     /locus_tag="PKO06_14375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNV70884.1"
                     /translation="MEFHLIPLDRVHINPRQPRKFFDSEKIEELAQSIHANGILQPIL
                     VRAKGDRYEIISGERRYRAARIAGLDRIPAVIRSLADREAHFAAIIENLQREDLNPVE
                     EAQALREVILEYSLTHDQLAERLGKSRSAVTNLLRILQLPDDVQSLISNGKISPGHAK
                     ILVGLRDLKEIRRWVRQIVRENLSVYETERQLAHARTETKAKTEAGTKGKKAVTGDLH
                     VRHAEDNLMEALGAKVRIRQGRKRGVIEIEFYSPDDLERLIENLGSCRL"
     gene            1175..2026
                     /locus_tag="PKO06_14380"
     CDS             1175..2026
                     /locus_tag="PKO06_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV70885.1"
                     /translation="MPVPGKDTLHYGGNTTCIEIRTSDNRLIIIDAGTGIRALGLELL
                     KGEFGRGEGEAHILFTHTHWDHIQGFPFFLPAYIKRDPSAPSGKVTGNKLHLYGASDV
                     DDRLEATLRGQMEHFYFPVALDYLAAQITFNPLQGKSFQVGQAKITARRLIHPNGVLG
                     YRIEEANKVLTVATDCEHPTDGSIDNNLLELAQDADIFVYDAQYTPQEYNPSQHGGSG
                     PSKIGWGHSTPDEGIRIAHTANAKRLILTHHDPLHNDDFIRQMEQDTQSKFAESQFAF
                     EGLNLEI"
     gene            2123..>2571
                     /locus_tag="PKO06_14385"
     CDS             2123..>2571
                     /locus_tag="PKO06_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70886.1"
                     /translation="MGKGAFRTPVYLFGSTRSGVGKSTLLAQVAVYSHARGARTAIID
                     LNHAFPLQIRNALPRSLPLEVYADLSEISQQISPRFRRSFYFTNVDRLSYFPGFKIPG
                     PLALLQDTSYRDFFLQLRGSFDQVFISLPPGIQEFACLEMFASKRYAR"
BASE COUNT          576 a          776 c          657 g          562 t
ORIGIN      
        1 cccacgatcg ccagcaccca tcctgcgttc gcgattccct ttgtccgttt catcgtcgcc
       61 tcctcaaatc atgttcggtg gttttccact gaaagttcaa gctcccggat agtataccag
      121 tgtgtaacgg tacgttgaca acaactctca cggtctgcta ccttccggga agagactcaa
      181 tacacctgta ttcagagtgg gaagaccctc tccaacaatt tttcgtcagg ttcttcgatc
      241 atgataccga tatctatgac tgtccgtggt cgccgtgaaa ggatcatatg gaattccatc
      301 tgattccgct cgatcgggtg cacatcaacc cccggcaacc gcgcaagttc tttgactcgg
      361 aaaaaattga ggaactggct cagtcgattc atgccaacgg aatccttcag ccgatcctgg
      421 ttcgcgccaa gggagaccgg tacgaaatca tctcgggcga gcgacggtat cgggcggccc
      481 gcatcgccgg tcttgaccgc attccggccg tcatccgttc acttgcggac cgggaagcac
      541 atttcgcggc gatcatcgag aacctgcaac gcgaagatct gaaccctgtt gaagaggcgc
      601 aagccctgcg cgaagtgatt ctcgaataca gtctgactca cgaccagctc gccgagagac
      661 tcggaaaaag tcgctcggcg gtgaccaatc tgctgcgtat tctgcaactt cctgacgacg
      721 tccagtccct catcagcaac ggaaagatct cgcccggcca tgccaagatc ctggtcggat
      781 tgcgcgattt gaaagagatc cgccggtggg tacggcagat cgtacgcgag aacctttccg
      841 tatatgaaac ggaacgtcag ctggctcacg cccgcaccga aacgaaggcg aaaacagaag
      901 ccggcacgaa aggaaaaaaa gcagtgactg gtgacctcca tgttcgacat gccgaagaca
      961 acctcatgga agccctcgga gccaaagtcc ggatccgaca gggtcgcaag cgaggagtca
     1021 tcgagattga attttattcg cccgatgatc tcgaaagact tatagaaaac ttgggaagtt
     1081 gccgattata atgatcgagt agaatttttg actgtcagtt caggttgaaa ggtttcaaga
     1141 ggtgtatata aagttctggg gggtccgcgg ctccgtgccc gtaccaggga aggacaccct
     1201 gcactacggt gggaatacca cctgtatcga gattcgcacc tccgacaacc gactgatcat
     1261 tatcgatgcc ggcaccggca tacgcgctct gggcctcgaa ctgctgaaag gcgagttcgg
     1321 acgcggcgag ggcgaagccc acattctgtt cacccacacc cactgggacc acatccaggg
     1381 ttttcccttc tttcttcccg cgtacatcaa acgcgacccg tcggccccca gcggaaaagt
     1441 cactggcaac aaactgcacc tgtacggcgc ttctgacgtc gacgaccgtc ttgaagccac
     1501 tcttcgcggg cagatggaac atttctactt tccggtcgca ctcgattatc tggcggcaca
     1561 gatcacgttc aaccccctgc agggaaaatc ctttcaggtc ggtcaggcga agatcactgc
     1621 ccggcgattg attcatccga acggcgtgct cggttaccgg atcgaagaag ccaacaaagt
     1681 cctgaccgtc gccaccgact gcgagcatcc caccgacggc tcgatcgaca acaatctgct
     1741 cgaactggct caggatgcag atatcttcgt ctatgacgcg cagtatacac cgcaggagta
     1801 caacccgtcg cagcacggcg gttcgggtcc aagcaagatc ggctggggtc attccactcc
     1861 cgatgaaggc atccgcatcg cccataccgc aaacgcgaaa cgcctgattt tgacgcatca
     1921 cgacccactg cacaacgatg atttcattcg gcagatggag caggatacgc aatccaagtt
     1981 cgcggagagc cagtttgcct tcgaaggtct gaatctcgag atctgacttc ggttcagccc
     2041 cctcgttcgt tcctccaatc tctgaactcc cactgacttc cctccttccc cactttccgc
     2101 gcctgtggta ggatgacgcg gcatgggaaa aggggcgttc cgtacaccgg tttatctgtt
     2161 cggttcaacg cgcagcgggg taggcaaatc gacgttattg gcgcaggttg ccgtctactc
     2221 gcacgcgcgc ggagcccgca cggccattat cgatctgaac catgccttcc cgctgcagat
     2281 ccgcaatgcc ctgccgcgtt cgctgccact cgaagtctac gccgatcttt ccgagatctc
     2341 gcaacagatt tctcctcgtt ttcggcgcag tttctatttc acgaacgtcg accgtttgtc
     2401 ctactttccg ggcttcaaga tccccggccc gcttgccctt ttgcaggata cgagttaccg
     2461 cgatttcttt ctgcagctgc gcgggtcatt cgaccaggtg ttcatcagcc tgccgccggg
     2521 cattcaggaa ttcgcctgcc tcgagatgtt tgcctccaaa cggtatgcgc g
//
LOCUS       DAORHD010000711         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48178_length_2570_cov_3.172962, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000711 DAORHD010000000
VERSION     DAORHD010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48178_length_2570_cov_3.172962"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKO06_14390"
     CDS             complement(<1..549)
                     /locus_tag="PKO06_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70887.1"
                     /translation="MYCHEAFRCPREDCPVRRRHLRRCWEYFGAQGLRVTLELCPYAP
                     CDRCHYRMGWEIGLIGDSLFTEDELAAEPSAAQPAPLKTADPQAAETPNPETLSVENP
                     SAGVPIGQAELPVLPESATSSTPDRLPESSPAPAEPQIKTSSLPPFPPEVETGQSVND
                     RPSGDVPEGASQGLRQADTSAEK"
     gene            complement(1162..1959)
                     /locus_tag="PKO06_14395"
     CDS             complement(1162..1959)
                     /locus_tag="PKO06_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV70888.1"
                     /translation="MSQTNAALILDQIWKKYGQFEAVKGVSLEIPRGELFGFLGPNGA
                     GKTTLIRIINGILQPTSGRVVICGHDLDQQPEEAKRSIGYIPDRPYLYEKLTPLEYFE
                     FIAGLYSIPAEQYLRRGEEMLNRFSMWEWRNELIESFSHGMKQKIAMTASILHDPDLL
                     VVDEPMVGLDPRSVRLVKDFFLEMVKTGKTIFLTTHTLSVAESLCHRIGIINRGQVVA
                     MGSMDDLRGLSKGSNVDLESVFLKLTEEESDDRQDTLPPGSPCDLTT"
     gene            complement(1946..>2570)
                     /locus_tag="PKO06_14400"
     CDS             complement(1946..>2570)
                     /locus_tag="PKO06_14400"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70889.1"
                     /translation="PALYSGEIYDSLVFFNGPFIGFIVSAISMRFVYPSISLEGRAFW
                     AVKASPIRPSRLLMIKFFLYLLPNLLLSLILCTVSNALFETTSPTLRFLSFLNVTLMA
                     LVTTALAIGIGAVFAQFNSDNPLKIAGSFGGAVFMVASSLYIFNLLVVESYPMYRFYF
                     RKFMPLYDRQGWILIAISFLLLLLCTLAWVLVPLARGREALERYEPD"
BASE COUNT          614 a          734 c          716 g          506 t
ORIGIN      
        1 cttttccgcg cttgtgtctg cctgtctcag accttgggaa gctccttccg gcacatctcc
       61 actcggacga tcgttcaccg attgtcccgt ctccacttcg ggtgggaagg gcggcaacga
      121 actcgttttg atctgtggct ccgcaggagc tggcgacgat tcaggcagac gatccggcgt
      181 agaactcgtg gcagattccg gcaggacggg gagctctgcc tgaccaatgg gcacacccgc
      241 agacgggttc tcgacactca gtgtctcggg gttcggcgtc tcagcagcct gcggatcggc
      301 cgtcttcagt ggcgcgggtt gagcagcact cggttcggca gcaagttcgt cttcggtgaa
      361 caggctgtct ccgatgagac cgatctccca gcccatgcga taatgacagc ggtcacacgg
      421 agcatacgga cagagttcga gagtaaccct caatccctgc gccccgaaat actcccagca
      481 gcggcgcaga tggcggcggc gaaccgggca gtcttcacgc ggacaccgga acgcttcatg
      541 acagtacata ggtgcgtgac cgtttcaggg aacctgtttg atccgcactg tggaacgctc
      601 ttccttccgg cctgtattcg gccagatggc caggaggatc gggacacacc tgcagatgcc
      661 gcaccctgtc aatctctcgt ggagaagttg gacgtggtac cacgatccgg cgtgaggctg
      721 cagattatgc gtcagtgcca tttcagatgc agagatcctc gtcaagagtc gattccagac
      781 cctgtgggca aaagccgtgc agagaacgga aaggtacaga cccgaaaaca ggactttagc
      841 atttcagttt accacaaacg acacacaggt tccatcacgg caaaggctgt ggaagaaggt
      901 tcagaacacc gtggcagcct gtgtttcaca cggtgacaat gcggctgccc aagcgagcag
      961 gacgacgcca gaggagtcgt tgagatgcac gcatcgacac gccgccatca ccacccgctc
     1021 acgaggcaac cagaacaggt caggcaattc ggcgaaacgt cagacggggc acacagtgtc
     1081 ctgacgtctg ctccgaccag cctcacggcc atcagagtca gaatcaccag aacaggggtg
     1141 tgtctcaaga accgctccag ttcaggtggt caagtcgcac ggtgatcccg ggggcagagt
     1201 atcttggcga tcgtcagatt cctcttcggt cagcttgagg aaaacgctct cgagatcgac
     1261 gttcgaaccc ttcgacaaac cgcgcagatc gtccatgctt cccatcgcca cgacctggcc
     1321 tcgattgatg atgccaatgc gatggcacag agattctgcg accgaaagcg tatgtgtcgt
     1381 cagaaagatg gtttttccgg tcttcaccat ttcgagaaaa aagtctttca ccagccggac
     1441 gctgcgcgga tcgagaccaa ccatcggttc gtccacgacg agcagatcgg gatcgtgcag
     1501 gatcgacgcc gtcatggcaa tcttctgttt catgccatgc gaaaagcttt caataagttc
     1561 gttacgccat tcccacatgc tgaatcggtt cagcatttcc tcacctcgtc gcaggtactg
     1621 ttccgcggga atggagtaca gaccagcgat gaactcgaag tattctaacg gggtcagctt
     1681 ctcatacaga tatggtcggt caggaatgta cccaatcgac cgtttcgctt cttccggctg
     1741 ttgatcgaga tcatgaccgc agatgaccac tcgtccggaa gtcggctgca gaatcccatt
     1801 gatgatcctg atcagcgtgg ttttcccggc tccattcgga ccgagaaagc cgaacaactc
     1861 ccctcgcgga atctccagcg agaccccctt gaccgcttcg aactgtccgt atttcttcca
     1921 gatctgatcc agaatcagcg cggcattagt ctggctcata acgttccagc gcctcccttc
     1981 cgcgtgccag gggcaccagg acccaggcga gagtacaaag caacaacagc agaaagctga
     2041 tggctatgag aatccagccc tgtcgatcgt ataagggcat gaatttgcga aaatagaatc
     2101 gatacatcgg gtacgattcg acgaccagca gattaaagat gtagagcgag cttgcgacca
     2161 tgaagaccgc gcctccgaat gatccggcga tcttgagcgg gttgtccgag ttgaactgcg
     2221 cgaacacggc gccaattcct atcgccaacg cggtcgtgac caacgccatc agggtgacgt
     2281 tcagaaaact caggaaacgc agcgtggggc tcgtggtctc gaacaaggcg ttggacaccg
     2341 tgcagagaat cagcgacagc agcagatttg gcagcagata cagaaagaac ttgatcatca
     2401 gcaggcgact cggacgaatc ggggaagcct tgaccgccca gaatgcccgc ccttcaagac
     2461 tgatcgaggg gtagacgaat cgcatcgaga tcgccgagac gatgaacccg atgaacgggc
     2521 cattgaagaa aacgagactg tcgtagatct cgccagagta caaagcagga
//
LOCUS       DAORHD010000712         2570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48184_length_2570_cov_3.003181, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000712 DAORHD010000000
VERSION     DAORHD010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48184_length_2570_cov_3.003181"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKO06_14405"
     CDS             complement(<1..702)
                     /locus_tag="PKO06_14405"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV70890.1"
                     /translation="MLEFLLLVPMTLGLIAFAVRHKELLQYLLVIGGSLHAVATALLW
                     NSGSLSTLGGWIAVDAAGLLFLSVTSGLFLLVAFFTVGHLRGTDMHYPAGHQAVFVGC
                     FLLFLSTMTLTCLSQHLGLLWVAIEATTLASAPLIYFHRSSRSLEATWKYLVICSVGI
                     ALALLGNFFLVLSSTGTGSTVSSLVLSDLIRAGPTLLTPWVKVAFVFFLVGFGTKMGL
                     APMHTWLPDATRKKTN"
     gene            complement(707..1354)
                     /locus_tag="PKO06_14410"
     CDS             complement(707..1354)
                     /locus_tag="PKO06_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNV70891.1"
                     /translation="MTGIIETLLIFIVLSNLLLLGSSRIGVYIKVVALQGIMLAGMLF
                     LAHSERHLHLLFLILVTMILKGWVFPRLLFQAVRQAGVRREVEPFVGFNLSLLIGIAG
                     WGFSLWIGGRLPLPETLGTDLTVPTAMFTIFVGMFLIIARRNAITQVLGYLVMENGIY
                     VFGAAVAGAQPFLVEMGMLLDIFVAVFVMVITLFQINREIGEIGMDTSRLATLKD"
     gene            complement(1356..2273)
                     /locus_tag="PKO06_14415"
     CDS             complement(1356..2273)
                     /locus_tag="PKO06_14415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNV70892.1"
                     /translation="MVELHPSGFLSLVIALTSAPLLLSVINRTKAWFAGRQGPPWLQP
                     YSDLYKMLGKGAVYSHTTTWMIVAGPVVGLACTLCCLTLLPWGGVPAVFSFSGDVILL
                     AYLLGLMRFFTVLAALDTGSSFEGMGASREVTFSALAEPTLFIGFAILAVQTGSLSLT
                     GMAVKVDVDLWLQAGSVIALVACAWFAVFLAENARIPVDDPNTHLELTMIHEVMVLDH
                     GGVDLAFIMYAAALKMWVLGTLLITLLIPRVTPWYLHLISAFCGMLLLALLTGVIESL
                     MARLRMNRVPQMLVGAMVLAALALILLVR"
     gene            complement(2267..>2570)
                     /locus_tag="PKO06_14420"
     CDS             complement(2267..>2570)
                     /locus_tag="PKO06_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HNV70893.1"
                     /translation="AVPTARMSYSAGAFTQPLVDVFGFFLRPFRHVPELPGLFPPAST
                     HVVKIEDVSRERLLRPVFQTVGHVLGKLRWLQEGRLQIYVLYITLTLLVLMGWNLW"
BASE COUNT          730 a          739 c          702 g          399 t
ORIGIN      
        1 gtttgtcttc ttcctggtcg catcgggcaa ccaggtgtgc atcggagcca gacccatctt
       61 ggtgccgaat ccgaccagga agaagacaaa cgccaccttc acccacggcg tcagcagcgt
      121 cgggccggcg cgaatcagat cagaaagtac aagcgatgag actgtagaac cagttccagt
      181 cgaagacaag acgagaaaga agtttccgag caaggccagg gcaataccga cggaacagat
      241 caccaggtat ttccaggtgg cttcgagtga tcgtgaactg cgatgaaagt agatcagcgg
      301 cgcgctggcc agagtggtgg cttcaatcgc cacccagaga agccccagat gctgactgag
      361 acaggtcagc gtcatggtcg acagaaacag gagaaaacac ccgacaaaga cggcctggtg
      421 tccggccgga tagtgcatgt ccgttccgcg caggtgacca acggtgaaaa aggccaccag
      481 aagaaacaga ccgctggtaa cactgagaaa cagcagcccg gccgcatcga ccgcaatcca
      541 gccgccaagc gtcgacagtg aaccgctgtt ccagagaaga gccgtggcga cggcatgcaa
      601 cgagccgccg atgaccagaa ggtactgcag cagttccttg tgacgaacgg caaaagcgat
      661 caaccccaac gtcatgggta caagtaaaag aaactccaac atggcatcaa tccttaagcg
      721 tcgccaggcg cgacgtatcc ataccgattt cgccgatctc ccgattgatc tgaaacagcg
      781 tgattaccat cacgaagacc gcgacaaaga tatcgagcag cattcccatt tctacgagaa
      841 acggctgggc gccggcaacg gcggcaccaa acacgtaaat gccgttttcc atgaccagat
      901 agccaagcac ctgggtgatg gcatttcgac gcgcgataat cagaaacatg ccgacaaaga
      961 tcgtgaacat cgccgtcggc acggtcagat cggttccaag tgtttctggc agcggcaatc
     1021 gtccgccgat ccagagggaa aacccccagc cagcaatgcc gatcagcaga gacagattga
     1081 aaccaacgaa tggttcgact tcgcgtcgaa ccccggcctg ccgtacagcc tgaaagagca
     1141 ggcgcggaaa cacccagccc ttcaagatca tggtcaccag tatcagaaag agaaggtgca
     1201 gatggcgctc gctgtgggcc agaaacagca taccggcgag catgatgccc tgcagggcca
     1261 ccaccttgat gtagactccg atgcgactcg atccgagcag caacaggttc gagagaacaa
     1321 tgaagatcaa cagggtttcg atgattccag tcatgtcagc gcaccagaag aatgagtgcc
     1381 agagcagcga gaaccattgc tccgacgagc atctgcggca cacgattcat ccgcagacga
     1441 gccatcagtg attcaatgac ccccgtcagc aaggccagaa gcaacatgcc gcagaaggct
     1501 gagatcagat gcagatacca gggcgtcact ctcggaatga gcagagtgat caacaaggtt
     1561 cccagcaccc acatcttgag agcggcagcg tacatgatga acgcgagatc gacgccccca
     1621 tgatcgagaa ccatcacctc atgaatcatg gttaactcga gatgtgtgtt tggatcatca
     1681 acaggaatgc gggcattctc agcgagaaaa accgcgaacc aggcacaggc gacgagagca
     1741 atgactgatc cagcctggag ccagaggtcc acatcgacct tcaccgccat gccggtcaag
     1801 gacagagaac ccgtctgaac ggcgagaatc gcaaagccaa tgaagagggt cggttcagcc
     1861 agggccgaaa acgtcacttc gcgactggct cccatgccct caaagctcga cccggtatcg
     1921 agtgcggcaa gcacggtgaa gaaacgcatc aaccccagaa ggtaggccag aagaatcacg
     1981 tctcccgaga aagaaaacac tgccggaacc cctccccacg gcaaaagggt cagacagcag
     2041 agcgtacaag ccaggccaac gacggggccg gcaacgatca tccaagtggt cgtatgactg
     2101 tagacggcac ccttcccaag cattttgtac aaatcagaat acggctgcaa ccagggaggg
     2161 ccctgtcttc cggcaaacca ggctttggtg cgattgatca cggacagcag cagaggagcg
     2221 ctggtcagcg cgatcaccag actgagaaat ccgctcggat gaagctctac catagattcc
     2281 accccatcag gaccagcagc gtcagtgtga tatacagcac ataaatctgt aatcggcctt
     2341 cctgaagcca gcgcagcttc cccaatacgt gaccgacggt ctggaacacc ggccgcaaca
     2401 aacgctcgcg cgagacatcc tcaatcttca cgacatgtgt cgaagccggc ggaaacaaac
     2461 ccggcagttc aggcacatgt cgaaaaggtc gcaggaagaa gccgaaaaca tcgaccaatg
     2521 gctgtgtgaa ggctccggct gagtaggaca ttcgcgctgt cggtacggcg
//
LOCUS       DAORHD010000713         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48290_length_2567_cov_3.205812, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000713 DAORHD010000000
VERSION     DAORHD010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48290_length_2567_cov_3.205812"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKO06_14425"
     CDS             complement(<1..644)
                     /locus_tag="PKO06_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70894.1"
                     /translation="MKSRFSVGLVVWCVLAVVFLSSDCRACDASFQTRVADLRTQLAD
                     LSSTLRPGQSETGAPARATTLRRDIEQLAGNASSDHLKIYRALELEAQRLDFALTQKD
                     SSASARHLKNADTLLQVLAARTPEVCTRDRHGHNGGMVSKPCAFCGAKGKDYWGTLCK
                     ICGGDGYVMAPKDSPACAGCQGKGKDYWGTVCQGCAGTGWANATIDSGDDDHGS"
     gene            978..2171
                     /locus_tag="PKO06_14430"
     CDS             978..2171
                     /locus_tag="PKO06_14430"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70895.1"
                     /translation="MTIFMLAMAGGNMVLAGQKATPGDIHSAAMCGDIEELRRILKTD
                     PQKVNLKAADGATPLHRAVNADQKKAVGLLASVGADLNARGPDDATPLIDAASNGNQM
                     IVKFLIGKKADAALVDKNGDTPLHRAARKGHVEVIKELLKHGVDASVKNKQGKSPADL
                     AQTMGHAGALALLPVPAATAKTPDPEPTVATEAAKPAVEPTKSATTSPEVEAYTGKGF
                     DLNNQGKFDEALKYLNEAIAKDPNFPGAYINRGFSYRNLGKMNEALADYNKALEFDST
                     SLDAAKAFANRGVVYAKTGKLEEAEEDYSRSLSISPDDPQTYFNRGSLGMRKGDVQAA
                     IDDFTTGLNMKPDDGYALLQRGICYQNSDRLDDARADIERAKELGIPEAADILKKLPA
                     EPEGD"
     gene            2325..>2567
                     /locus_tag="PKO06_14435"
     CDS             2325..>2567
                     /locus_tag="PKO06_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70896.1"
                     /translation="MKFSRLTPLFLGISLVALLLFINYYEQSTKKRPHTVSPFGRSTT
                     GSHVPMPPLRARIPVPSPPKTRPVVRVPITASYPSDA"
BASE COUNT          590 a          745 c          750 g          482 t
ORIGIN      
        1 tgactgccgt gatcgtcgtc gccggaatca atggtcgcat tggcccaacc ggtgccggca
       61 cagccctggc agacggtacc ccagtagtct ttgcctttgc cctgacagcc ggcacaggcg
      121 ggtgaatctt tgggtgccat gacatatccg tcaccaccgc agatcttgca gagagttccc
      181 caatagtctt tgcctttggc accgcagaaa gcgcacggtt tcgacaccat gccaccgttg
      241 tggccgtggc gatcacgggt gcagacttcg ggcgttcggg ccgccagcac ctgcagcagc
      301 gtgtcggcat tcttcagatg ccgggcgctg gccgatgagt ctttctgggt cagcgcaaaa
      361 tcgagacgct gcgcttcgag ttcaagcgcg cgataaatct tcagatgatc ggaagaagcg
      421 tttccggcaa gttgttcgat gtcgcggcgc agtgtcgtgg cacgagccgg ggcgccagtc
      481 tcagactgac cggggcgcag agtcgagctg agatcagcca gctgcgttcg caggtcggca
      541 acccgagttt gaaacgaagc atcacacgcc cgacaatcgc tcgacagaaa aaccacggcc
      601 aacacacacc aaaccaccag acccacggaa aaacgactct tcattgtgat gctccagaga
      661 cttaagactg gttgcccgct ctcacgacac gagcgcaaga gggtaatagc aaatcagatg
      721 ccagatctgg cctccgctgt tgatggggaa gaagcaggcc cttgcccgaa aaactgccca
      781 atcgttggca ccggttcgat gaatgaccgg cagaagaaaa cgccctgtac cgacaagatc
      841 aagatttgaa cgggaacgcg cgaaggagta tgctcgaaag gtcgagaaac gcatgcgatg
      901 ccctcaagtt acaggagtgg gggaggttcc cgatgttcac gtgtattcgt cgcactgtac
      961 cggttgcatt ctgtgtgatg accatattca tgcttgccat ggctggcggg aatatggttc
     1021 tggctggcca gaaagcgact cccggggata ttcattccgc tgccatgtgt ggtgacatcg
     1081 aagagctgcg acggattctc aagaccgacc cgcagaaggt gaacctgaaa gcggctgatg
     1141 gcgccacgcc gttgcatcgg gcggtgaacg ctgatcagaa gaaggctgtc ggcctgctcg
     1201 cctcggtggg tgctgatctc aacgcgcgcg gtcctgatga tgccacgccg ctgatcgatg
     1261 ccgcgagcaa cggcaatcag atgatcgtca agttcctgat cgggaagaaa gccgatgccg
     1321 cgctcgtcga caagaacggg gacacgccgc tgcaccgggc ggcgcgcaaa gggcatgtcg
     1381 aggtcatcaa agagctgctc aagcacggag ttgatgcgag tgtgaaaaac aaacaaggca
     1441 agagcccggc cgacctcgca cagaccatgg gtcacgccgg tgccctggca ctgctgccgg
     1501 ttccggcagc cacggccaag acaccagacc ctgaacccac cgtcgcgacc gaagcggcga
     1561 aacccgcggt cgagccaacg aagtctgcca caacatcgcc ggaggttgag gcatacaccg
     1621 ggaaggggtt cgacctgaac aatcagggca agttcgacga agctctcaaa tacctgaacg
     1681 aagccatagc gaaagatccg aacttccccg gggcgtatat caatcgcggg ttttcgtatc
     1741 gcaacctcgg caagatgaac gaagcgctcg ctgactacaa caaggctctc gagtttgatt
     1801 ccacctcact tgatgcggcc aaggctttcg cgaaccgggg cgtcgtctac gccaagaccg
     1861 gcaagcttga agaagccgaa gaggactatt cccgttcgct cagcatctcc cctgatgacc
     1921 cgcagaccta ttttaatcgc ggctctctgg gcatgcgcaa gggcgatgtg caggcggcga
     1981 tcgacgactt caccactggt ctcaacatga agcccgatga tgggtacgcg ctgttgcagc
     2041 gcggcatctg ctatcagaac agcgatcgtc tcgacgatgc ccgggccgac atcgaacgcg
     2101 ccaaggaact gggcattccc gaagctgccg acattctgaa aaaactgccc gccgaacccg
     2161 agggtgactg accatgaatt gcgcatcggc agatgcagaa gatcgagggc agccgagacg
     2221 cagactcaag cttgtggtga aagttgctcg ctggcctggt cgttcggaga tcatcgagtt
     2281 tctggcggct ctggattgaa cgtgcgcagg aggagaagga gttcatgaag ttcagtcgac
     2341 ttacgccgtt gttcctcgga atcagtctgg tggctctttt gctctttatc aactactacg
     2401 aacaaagcac gaaaaagcga ccgcatacgg tttctccgtt cgggcgttcc accacgggtt
     2461 cacatgttcc catgccaccc ctgcgtgctc ggattcccgt tccttcgcca ccgaagacga
     2521 gaccggttgt cagagtaccg atcaccgcat cctatccttc ggatgcg
//
LOCUS       DAORHD010000714         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48291_length_2567_cov_3.068073, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000714 DAORHD010000000
VERSION     DAORHD010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48291_length_2567_cov_3.068073"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..511)
                     /locus_tag="PKO06_14440"
     CDS             complement(47..511)
                     /locus_tag="PKO06_14440"
                     /inference="COORDINATES: protein motif:HMM:NF023374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HNV70897.1"
                     /translation="MSKRPPELTWQDKAILIVIPVVVLVIFVLANHMTQDNTVSVAFT
                     EKLPKTEVRDSGTIEEVLPSEVASGTQIQKCRVRTRDGQVVFTLEYRVVGSETMNLQV
                     GRLIQVFGEYQYDLTGGKLLFPYKGKSGQPLGWAVYENRRYHATESPSKDQL"
     gene            723..2333
                     /locus_tag="PKO06_14445"
     CDS             723..2333
                     /locus_tag="PKO06_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV70898.1"
                     /translation="MWSKLRNRHYSILALTAIVALETSAIAVDRLFAKPNDTAFEVVG
                     TFRNNGLINQYRSADGKASPVPANLSAFQKTIPPAAATTRMAASSLPSITIPGARASE
                     RAAAPSITISDRADALRLATPENWVEYSVNRGDSLSSIASIFGSTSEAISQANGINDN
                     RNLKSGQKIKVPVATPRVLYTVRDGDTLNRIASRFNVSLQELIHANNLKNHLLVTDQK
                     LSIPTRVQSSQLELVKAEPRQSLTKPTLTIATGPKLSLVKVEPIQMIAPAKPTLTIAP
                     FDSSTAAANRLQTNAAAPVAVPAPVKLTPPLPAVAPAPAVVKAVPAEKPIIAVKQTIQ
                     AAPATTPAAQLVKHTVKSGESLSSLARQYGTSISEIVASNQLTSSNLKPGTQVRIPTT
                     RKQYKVTQGTSHRTNVRTGMAMPVRGRLSDGFGWRKHPVYRRRLFHSGIDIAAPRGTP
                     IMSALPGTVVYSGWMSGYGRLVVIRHANGLSTRYGHCSKLNVRKGQTIRSGQVVGLVG
                     ATGTATGNHLHFEVRSNGKAVNPMTYLR"
     gene            2384..>2567
                     /locus_tag="PKO06_14450"
     CDS             2384..>2567
                     /locus_tag="PKO06_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70899.1"
                     /translation="MKRLILPLIIASGAVFLFSTPTLVAAEKGKSSGAQTTTAALGRN
                     LAPQAPAEGLPVFNPRY"
BASE COUNT          543 a          850 c          666 g          508 t
ORIGIN      
        1 tatgaccagg gtcgtgtcaa cgaaatgtac gggaaccggc gcacacttac agctgatctt
       61 tggaagggct ttcggttgca tgatagcgac ggttctcata caccgcccac cccaggggct
      121 gaccgctttt gcccttgtaa gggaacagta acttcccgcc ggtcagatca tactgatact
      181 caccgaacac ttggatcagc ctgcccacct gcaggttcat ggtctctgac cccaccacgc
      241 gatactcaag cgtgaaaacc acctgaccat ctcgcgtccg cacgcgacat ttctgtatct
      301 gggttcctga cgccacttca gacggcaaca cttcttcgat cgtaccggaa tcacgcactt
      361 cggttttggg cagcttctcg gtgaacgcaa cgctgacggt gttgtcctgg gtcatgtgat
      421 tggccagaac gaagatcacc agaacgacaa cgggaatcac gatcagaatg gctttatctt
      481 gccaggttaa ctcgggtgga cgcttgctca cagacggttc tcctcgtgca gacatgactt
      541 gtaatgaggc tcgcagctta ccacagacaa tttttctccg tccagtgcca tgtgacgagt
      601 tcttcactgt caagtttctc gcgttgtttg ccgatgaatt gtctacgtga ttcccgatgg
      661 ccaaggaagg cctgcccgca gggacgcaaa gagacagcag accactgttc aaggagtgca
      721 ttgtgtggag taaacttcga aaccgacact actcgatcct cgcgctgacg gccattgttg
      781 ctctggaaac gtccgccatt gccgtggacc gcctgttcgc caagcccaat gatacggcgt
      841 tcgaagtggt cggcaccttc cggaacaatg gtctgatcaa tcagtatcgc tctgcggatg
      901 gcaaagcgtc ccccgtcccg gcgaatctct cagccttcca gaagactatc ccccccgcgg
      961 cggcaaccac ccgcatggcg gcttcctctc tgccttcgat cacgatcccc ggggcccgcg
     1021 ccagtgaacg cgccgcggcg ccgtccatca ccatctcaga ccgagctgat gcgcttcgcc
     1081 tggcgacacc ggaaaactgg gtcgagtatt ccgtgaatcg cggtgattcg ctgtcgagca
     1141 ttgcgagcat cttcggctcc acttccgaag ccatcagcca ggccaacggc atcaacgaca
     1201 atcgcaatct gaagagcggc cagaagatca aggttccggt cgccacgcct cgagttctct
     1261 ataccgttcg agacggtgat accctgaacc gcatcgccag ccgcttcaat gtctcacttc
     1321 aggaactcat tcacgcgaac aatctcaaga atcatctgtt ggtcaccgat cagaagctgt
     1381 cgattccgac ccgcgtgcag tcctcgcaac tggagctggt caaggccgaa ccccgccaga
     1441 gcctgacgaa gcccactctg acgatcgcca cgggtccgaa gctgtcgctg gtcaaagtcg
     1501 aaccgattca gatgattgct ccggcgaagc ccactctgac gatcgccccg ttcgattcca
     1561 gcacggccgc cgccaatcgt ctgcagacca atgctgcagc tccagtcgct gttcccgcac
     1621 cggtcaaact gactcctccc ctgcccgccg tcgccccggc gccagccgtg gtcaaggccg
     1681 ttcccgctga aaagccgatc attgctgtga aacagacgat tcaagcggca ccggcgacaa
     1741 cgcccgcggc ccaactcgtg aaacacacgg tcaaaagcgg tgaaagcctt tcgagcctgg
     1801 cacgccagta tggtacgagc atctccgaaa tcgtcgccag caaccagctg acttccagca
     1861 atctcaagcc cggcacgcag gtgcgcattc cgaccacgcg caagcagtac aaagtcaccc
     1921 agggaaccag ccatcgcacc aacgtgcgca ccggtatggc catgcccgtt cgcggccgcc
     1981 tcagtgacgg cttcggctgg cgcaaacacc ctgtgtatcg ccgccgcctg ttccactcgg
     2041 gcattgacat cgccgcgcct cgtggcactc cgatcatgtc cgcgcttccc ggcaccgtgg
     2101 tctattccgg ctggatgtcc ggctacggtc ggctggtcgt gatccgccat gcgaacggtc
     2161 tctccacccg ctacgggcac tgcagcaagc tgaatgtccg caaggggcag accattcgat
     2221 ccggccaggt tgttggcctc gtcggcgcga cgggcaccgc gaccggaaat cacctgcact
     2281 tcgaagtccg cagcaacggc aaagcggtaa accccatgac ctatcttcgc tagaacggag
     2341 acggctctcg gccagcgctt atgcgccaca gacacgccat cacatgaaac gactgattct
     2401 gcccctgatc attgcatcag gggcggtttt tcttttttcc acccccactc tcgtggcagc
     2461 ggaaaaaggg aaatcatccg gagcgcaaac caccacggca gctctggggc gtaatcttgc
     2521 tccacaggct cccgcagaag ggctgccggt cttcaacccg cgctatc
//
LOCUS       DAORHD010000715         2564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48385_length_2564_cov_2.489438, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000715 DAORHD010000000
VERSION     DAORHD010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48385_length_2564_cov_2.489438"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1068
                     /locus_tag="PKO06_14455"
     CDS             79..1068
                     /locus_tag="PKO06_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70900.1"
                     /translation="MAITRCNKCAHLQEQPDDQLGITVACPRCGNPTPVYSTLFFVGK
                     LLEKYFSAQREILRLQAQTMAGNPQSPGAQPQALDAIHLSNTDHLACEQQHEPIVDWF
                     GRKQIKVQANLRGVDTTGFFDEVAMLIGANLPVLKEVLERIRWAQQKAYTSTTIAFDK
                     KSPEDVQAIASFCQQLYDFSFVAKYLPNRQKNNVLLALQTAPTIRDFFNGEWLEWFAL
                     MTCLEYGKERQKRFSCARNLSITLTNNESHELDVFMLIDGQIPVCIECKTGEFRHDID
                     KYLNMRKRLGVSGKNVVMCVAGLSDEHAKGLTAMYDLTFVNERDLSTHLTRLY"
     gene            complement(1251..1847)
                     /locus_tag="PKO06_14460"
     CDS             complement(1251..1847)
                     /locus_tag="PKO06_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV70901.1"
                     /translation="MTALRTLALTLGSVAVATFATVANAFPIASSGEGASVLVGGTSD
                     IVATYQGNSASFSNDLYLMWDGAAGKPGDDGNLSNDVFIFNNHSSAVGSTVNLGAFAP
                     GTELEFRLHVNNTDDDFFTGAASRNPDSHAHARVQANWEPNTTLVSFEDLFNGPFDFN
                     DLSFSFTNTVSSVPEPATLALFGLGLLGLASAKRKNKR"
     gene            complement(2273..>2564)
                     /locus_tag="PKO06_14465"
     CDS             complement(2273..>2564)
                     /locus_tag="PKO06_14465"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNV70902.1"
                     /translation="GSLLDQFQRAGFGEQVSSWVGGGQNLPISPAAIAQVFGGEALAQ
                     VAQQAGVSEAEASEGLSQLLPEVFDRVTPDGQVPNLEQLAASAAALTRQFSG"
BASE COUNT          574 a          783 c          710 g          497 t
ORIGIN      
        1 tgccgaagac caggcagacg actcgaaccg gtcatcacct cttggcattc caccactcac
       61 ccgtcctggt tttcagtcat ggccataacg cgctgcaaca agtgcgccca cctgcaggaa
      121 cagcccgacg accagctcgg catcacggtc gcctgccccc gctgcggcaa tccgactccg
      181 gtttacagca ctctgttctt tgttggcaag ctgctcgaaa agtatttctc tgcccagcgc
      241 gagattctcc gcctgcaagc acaaaccatg gccggcaacc cacaatcacc aggcgcacag
      301 ccgcaggcgc tggacgcaat ccatctctcc aataccgacc acctcgcttg tgaacagcag
      361 cacgagccca tcgtcgactg gttcggccgc aagcagatca aggtccaggc caatctccgc
      421 ggggtcgata ccacaggctt tttcgacgaa gtggcgatgc tcatcggcgc caacctgccg
      481 gtcttgaaag aggtactcga acgcatccgc tgggcgcagc agaaggccta tacaagcacg
      541 acgatcgcct tcgacaagaa atcgccggag gatgtgcagg ccattgccag cttctgccag
      601 caactctacg atttctcttt tgtggccaag tacctgccca accggcagaa aaacaatgtc
      661 ctgctggccc tgcaaacggc cccgaccatt cgcgatttct tcaacggcga atggctggaa
      721 tggtttgccc tgatgacttg cctggaatac ggcaaggaac gccagaaacg cttctcctgt
      781 gcgcgcaacc tgagtatcac cctgaccaac aacgaatccc atgaactcga tgtcttcatg
      841 ctgatcgatg gccagatacc cgtctgcatc gagtgcaaga ccggtgaatt ccgtcatgac
      901 attgacaaat atctcaacat gcgcaagcgc cttggcgtca gcggaaagaa cgtggtgatg
      961 tgcgtcgccg gcctcagcga cgaacacgcc aagggactga ccgcgatgta cgacctgact
     1021 ttcgtcaacg aacgggatct gtcaacccac ttgacgcgcc tgtactgacg cccaccacga
     1081 atggcactcc acagcacgaa ccatttgcgc ggcaatcggt gggctgctgc gctgccgacc
     1141 agatttggca ggtcggctgg tggcggaatc agttcaccgg acttgatcag gcacaaaaaa
     1201 aaccccggac gcgccggggt ctggttggtg cctggtgccg cactaaagtg tcagcgtttg
     1261 tttttccgtt tggccgatgc cagaccgagc aagcccagtc cgaagagcgc cagcgttgcg
     1321 ggttcgggta cgctggatac cgtattggta aacgagaagc tcaggtcgtt gaaatcgaaa
     1381 ggcccgttga agaggtcttc aaagctgacc agagtcgtat tgggctccca gttcgcctgc
     1441 acacgcgcat gcgcatggct gtccgggttg cggctggcgg cgccggtgaa gaagtcatcg
     1501 tccgtgttat tgacatgcag gcgaaactcc agctcggtgc caggggcaaa agcgccgagg
     1561 tttacggtgc tgcctaccgc cgaggagtga ttgttgaaga taaacacgtc attggacaga
     1621 ttgccgtcat cacccggttt gccggcagcg ccatcccaca tcagatacag gtcgttgctg
     1681 aaggaagcgg aattgccctg ataggtagcg acgatgtcac tggtcccgcc caccagaaca
     1741 gaggcccctt cgccggaact ggcaatcgga aaagcatttg cgacggtggc aaacgtggcg
     1801 acggccacag accccagcgt cagggccaaa gtacgcagtg cggtcatttg catatctcct
     1861 tatttgtgtc gggcatttcg aacccacgga caggcgtgtt cgacaagagt tggtctttgg
     1921 gaataatcat gtgcagactt gaacacgaca tgtcctgtaa cagaagcacg gttattgccc
     1981 cgctacacaa acaaaagcaa acttcatgcc atagtttgaa agcatcatgg gaagatgttt
     2041 tatatccgat tgttatgtaa cccatgccgg agccagcacg tgaccgccgg gttcgatatg
     2101 cggtgcatct gtaagaaaag tcgacaggaa tcgagcaagg ctgtgccgct gtgccgactt
     2161 atgccgggag ggtgctgcgg caggtcaacc gacgcttttg catccaattt cctcagacgg
     2221 cctactgcaa gtggacggaa agcgtcgtac ccgcatcacc gatccggcag actcatccac
     2281 tgaactgccg agtcagggcg gcagcgctcg ccgcgagttg ctcaaggttc ggaacctggc
     2341 cgtccggcgt gacgcgatcg aacacttccg gcaacagttg cgacagtccc tcggatgcct
     2401 cggcttcgct cactcccgcc tgctgcgcga cctgcgccag cgcttcgccg ccgaacactt
     2461 gcgcgatggc ggcgggcgag atcggcaggt tctgaccgcc ccccacccat gaactcacct
     2521 gctcgccgaa gccggcacgc tggaactgat ccagcaggct gccc
//
LOCUS       DAORHD010000716         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48433_length_2562_cov_2.764260, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000716 DAORHD010000000
VERSION     DAORHD010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48433_length_2562_cov_2.764260"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /gene="dnaJ"
                     /locus_tag="PKO06_14470"
     CDS             <1..1097
                     /gene="dnaJ"
                     /locus_tag="PKO06_14470"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNV70903.1"
                     /translation="PKNADENELKRAYRAMARKYHPDVNPNNPDAAEKFKELNEAYQV
                     LSDAKKRQMYDQFGHGGVDGQGFGGFEGFGGGFEGGIGDIFGDILSDFFGGSSSRGRA
                     GPRRGNDLRYDLDISFEEAVFGCEKTIHLEKNRPCTGCSGTGAQSGSGRKSCPVCRGK
                     GTISFSQGFFAIQKTCHQCQGEGSIIEKPCGTCRGTGRLRTPEQLQVKIPPGVETGSK
                     LKLSGEGEPGDRNGQPGNLYIVLNVGKHDFFERVGDDIVCEKPIALSMAVLGGEIEVP
                     TLKGKVKITIPPGTQPNKVFRLRGYGVKSLNGGTGDQLVRISVRIPTTVSKKQKELLQ
                     EFAKEEGEPPAVSTGENKSFFDKVKEALGG"
     gene            1358..2173
                     /locus_tag="PKO06_14475"
     CDS             1358..2173
                     /locus_tag="PKO06_14475"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV70904.1"
                     /translation="MKYRPRHAEEKLRKLARHFKVVLVTGARQVGKTTLLKHVFPKHR
                     LITFDPVQDLFGARSDPDFFLNSFPPPLILDEIQFVPELLAALKRRVDESEKPGQYLL
                     TGSQNLSVLRTVSESLAGRVALLPLEGMTYLEMIGRTERGNWLSHYLHDPVKFVKKLR
                     PAASTGTSVPDMLWRGSFPGTLDLSDDMIQDYFNSYLRTYVERDVRHIEGIRELTSFG
                     RFVSLASALTAQEVNASHLGREVKCPRIPVGSGWICSQPRTSGLKFRPITAIR"
     gene            2083..>2562
                     /locus_tag="PKO06_14480"
     CDS             2083..>2562
                     /locus_tag="PKO06_14480"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNV70905.1"
                     /translation="MSPNTGRKWLDLLAATYQWFEVPPYHGNTIKRVSGKTKGYFSDT
                     GFASTLHQISSPKALAMNPMLGPLFETWVVSTIRRLFCTIPSRPGMYHWRTAGGAEID
                     LVLELDDCLFPIEVKCKTHLSGNETRCFRAFRETYPKKRIMPGLILYAGTTPYRLNEL
                     "
BASE COUNT          617 a          658 c          718 g          569 t
ORIGIN      
        1 tcccgaaaaa cgccgatgag aatgagttga aacgggcgta ccgggcgatg gcacgcaaat
       61 accatccgga tgtgaatccg aacaacccgg atgccgcgga gaagtttaaa gaactgaatg
      121 aggcctacca ggtactcagc gatgcgaaga agcggcagat gtacgaccag ttcggacatg
      181 gcggagtcga tggccagggc ttcggcgggt ttgaagggtt cggtggcgga tttgagggtg
      241 gcatcggtga tatcttcggc gacattcttt ccgacttttt cggtggctcg agttcgcgcg
      301 gcagagccgg cccgcgtcgc gggaacgacc tgcgatatga tctcgacatc agctttgaag
      361 aagccgtctt cggctgcgag aagaccattc atctggaaaa aaaccgaccc tgtaccgggt
      421 gcagcggtac tggggctcag agcggcagcg gtcgcaagag ctgccctgtg tgtcggggca
      481 aaggaacgat ctcgttttct cagggttttt tcgccattca gaaaacctgt catcaatgtc
      541 agggtgaggg ctcgatcatc gagaaaccct gtggcacctg tcgtggcacc ggccggttgc
      601 gcacgcccga acagcttcag gtcaagattc cgcccggagt cgaaaccggc agcaaactga
      661 agctgtcggg tgagggcgag cccggagacc gcaatggaca gccaggcaac ttgtacattg
      721 tcttgaatgt cggcaagcac gatttcttcg aacgggtcgg cgacgacatc gtgtgcgaga
      781 aaccgattgc cctctcgatg gcggtacttg ggggagaaat cgaagtgccg acgctgaagg
      841 gaaaggtgaa aatcaccatt ccgcccggca ctcagccgaa caaagtgttt cgtcttcgag
      901 gctatggggt caaatcattg aatggcggca ccggcgacca gctggtacgc atctcggtgc
      961 gcattccgac cacggtctcg aagaagcaga aagaactttt gcaggagttc gcgaaagagg
     1021 aaggcgaacc gcccgccgtc agcaccggag aaaacaagag tttcttcgat aaggtgaaag
     1081 aagccctcgg cggctgaccg atcgtctggg tcagctcgtt tgcacaccag cactgaaagc
     1141 gaatatgaac attcatgttc gcttttcttt tttaggcgac ttgaacgcgg aaatgatcgc
     1201 gcacgagatg catgcgagca gacgttgtaa cgcaggaaag gattttcgat tcgatgaaac
     1261 aggacaattt tggtcgatta ccttccctga gaacatcctt gcaatattga agtgatagtt
     1321 atagtatgcc agaaagaatg tggtctggag acgcgaaatg aaatatcggc ctcgacatgc
     1381 cgaagaaaaa cttcgaaagc tggcccgtca tttcaaggtt gttctggtga ccggagctcg
     1441 gcaggttggg aaaaccaccc tgctgaagca tgtttttccg aaacatcgtc tgatcacctt
     1501 tgatccggtt caagaccttt tcggagcaag aagcgacccg gactttttcc tcaactcctt
     1561 ccctcccccg ttgattctcg acgagattca gttcgttccc gagcttctcg ccgctctcaa
     1621 aagacgagtc gatgaatccg aaaaacccgg tcagtatctg ctgaccgggt ctcagaatct
     1681 gagtgtcctg cgaacggttt ccgaaagcct ggccggtcgg gtagccctcc ttcccctcga
     1741 aggaatgacg tatctggaaa tgatcggcag gacagaacga gggaattggc tgtcacacta
     1801 tcttcacgat ccggtgaaat tcgtaaaaaa actgcgccct gccgcatcga ccggtacgtc
     1861 tgtccccgac atgctttggc gtggatcttt cccaggaact cttgatctat ctgacgatat
     1921 gattcaagac tacttcaatt cttatctgcg cacctacgtt gaaagagacg ttcggcatat
     1981 cgaaggaatt cgcgaactca cgagttttgg tcgatttgtc agtctggcaa gtgcgctcac
     2041 tgcccaggaa gtaaacgcct cccatcttgg acgagaagtc aagtgtcccc gaataccggt
     2101 cggaagtggt tggatctgct cgcagccacg taccagtggt ttgaagttcc gccctatcac
     2161 ggcaatacga taaaacgggt cagtgggaag acaaaagggt atttcagtga taccggcttt
     2221 gcctccacct tgcaccagat atcaagtccg aaggctctcg ccatgaaccc gatgcttggg
     2281 cctctctttg agacgtgggt cgtctcgacg atcaggcgct tgttctgtac tattccgagc
     2341 cggccgggca tgtaccactg gcgaacggcg ggaggtgcgg aaatcgatct tgttctcgaa
     2401 ctcgatgatt gcctgttccc gatcgaagtg aagtgcaaaa cgcatctttc cggaaatgag
     2461 acccgctgtt ttcgagcttt tcgggagacg tatccgaaaa aaagaatcat gccgggtttg
     2521 atcctctacg cgggaaccac accctatcgc ttgaacgagc tt
//
LOCUS       DAORHD010000717         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48574_length_2557_cov_3.077138, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000717 DAORHD010000000
VERSION     DAORHD010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48574_length_2557_cov_3.077138"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..>2557
                     /locus_tag="PKO06_14485"
     CDS             26..>2557
                     /locus_tag="PKO06_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV70906.1"
                     /translation="MATGSSLPEGLVAFTAADLAQNFATGPVWFQLVQVDASQPVVIG
                     ITPSTALGGSPVHTAAAQATFFDVEFSEPMSSGTVPAVKLVNGAFQWSLTASPTAPAC
                     WISSTTCRFTHTQPITATYPQGLYNYVVTGGRDYAGNTAVTSNGVFQLEVQSRGPIVT
                     SYLTQSQQATTASGTPWPTLDNQPFSFLVAPNAATLTVTLSTASGTVNSRVHFVQGNV
                     TVASVSVNWDGTSTIGTFTWDSATGPIPVNGSPYTLKFYDEFNNVSLETGIWTMDATA
                     PRVLATPTVSDTSTTTQVLYYSPSVTPNVNFAFRSDETSGLRVRLTGMGATDTWSMNK
                     QPGTLWTASFNGRFSNSTIATDGAYQFDVVDVAGNLGTASAGIGTSVAVIVDTIRPIV
                     SSYTLRAGGVGPIVTRFSTLAASLSIELTLDPSSPYGQTGVWEVDVLNDSGQVINRLP
                     LIASGANYVAWWNGTNFSGTTVLDGTYTFRARDAAGNKATLTSSVFAVTTPFKMLGGE
                     QVSSTAIRLFFNHPVDPLSLPTAVSFFNPATVTVSSYTLETAQNLIASLSGPLSHGVS
                     YSFAIATLTLRNLDGSPLAAGQNSFTFTADAAGPGVANPAVTFTGITTAREFFVNFNE
                     VVTAPTAVATANYVINGGAIPVTVVSLQTNRTSVRVTTAADLVSGTNYTLVINGVEDV
                     YHNLSAINTTFQGRDMVAPVFSVGVFSNPANEYDLIVAATVNEALAASPKSKVMQNGA
                     PSVEYTMTPGTLANSYMSGAHLDPNYPGAGWVEISGTDVAGNLGTTLASFTLAVVNAS
                     IRAEVKSSDGQFTSVFAAGSVKQKTAVKWLPRGNLTQNAAANVLVA"
BASE COUNT          500 a          755 c          727 g          575 t
ORIGIN      
        1 cactccgacc tcaggggttc atcgcgtggc cacgggaagc tcgttgccag aaggtctggt
       61 ggcattcacc gcggccgatc tcgcgcagaa cttcgctacc ggtcccgtct ggttccaact
      121 ggtccaggtc gatgcctcgc agccggtcgt gatcgggatc actccgtcaa cggctcttgg
      181 cggctctcct gtccatacag ccgcggcgca ggcgaccttc ttcgatgtcg agttctcgga
      241 accaatgagc tcaggaacgg ttccggccgt gaagttggtc aatggtgcct ttcagtggtc
      301 gctgaccgcg tctccgacgg ccccggcttg ctggatcagc tcaaccacct gtcgtttcac
      361 gcacacgcag ccgatcaccg ccacctatcc gcagggtctc tataactatg tggttacagg
      421 tgggcgcgac tatgccggaa acacggcggt gacctcgaat ggtgtcttcc agcttgaggt
      481 acagtcacgt ggaccgattg tgacttcgta tctgactcag tcacagcagg caacgacggc
      541 ttctggaacc ccttggccga cgttggataa ccagccattc agcttcctcg ttgcgccgaa
      601 tgccgcgacg cttaccgtga ctctctctac ggcatctggt acggtcaact ctcgagtgca
      661 cttcgttcag ggaaacgtca ccgttgcttc ggtcagtgtc aactgggatg ggacctcgac
      721 catcggcacg tttacctggg attcggctac cggtccgatt ccggtcaatg gatcgccgta
      781 cacgctcaag ttctacgatg agttcaataa tgtgtctctc gaaactggta tctggaccat
      841 ggatgcaaca gcaccacgcg tgctggcaac tccgacggta tctgatacct cgaccacgac
      901 gcaggtgctc tactacagcc cgagtgtcac gccgaacgtg aacttcgctt tccgatctga
      961 tgaaacgtct gggttgcgtg tgcgtttaac cggcatggga gctacggata cgtggtcgat
     1021 gaacaaacag ccgggaactc tctggaccgc cagcttcaat ggccgtttct cgaacagtac
     1081 catcgcgacc gatggcgcgt accagtttga tgttgtcgat gtggcgggga atcttggcac
     1141 cgcttcggct ggaattggca cgtcggtggc tgtgatcgtc gacaccatac gaccgattgt
     1201 ctcgtcttac acgctgcgag ccggcggagt tggcccgatc gtcacacgct tctcgactct
     1261 ggcagccagt ctgtccatcg aactgactct tgatccgagt tctccctatg gtcagaccgg
     1321 ggtctgggaa gtggatgtgc tgaatgattc gggtcaggtg atcaatcgat tgcctctcat
     1381 tgcctccgga gcaaactatg tcgcctggtg gaatgggaca aacttctcag ggacgaccgt
     1441 gctcgacggc acctatacgt tccgggcacg ggatgcagcc ggtaacaagg cgactttgac
     1501 cagttctgtc tttgcggtca ccactccgtt caaaatgctc ggcggtgaac aggtgagcag
     1561 cacggccatt cgactgttct tcaatcatcc ggtcgatcct ctgtctctgc cgaccgccgt
     1621 cagcttcttt aatccggcga ccgtcaccgt ttcgagttac accctcgaga cggcacagaa
     1681 tcttattgcc tccctgtccg gcccgttgag tcatggggtg agctactcct tcgcgattgc
     1741 cacactgacg ttgcgcaatc tcgacggctc tccgcttgcc gcaggtcaga acagcttcac
     1801 ctttacagct gatgccgcgg ggcccggagt ggcgaatccg gctgtcacgt tcacgggtat
     1861 caccaccgct cgagagttct tcgtaaactt caacgaggtg gtcacggcac cgacggcggt
     1921 cgcgacggcg aactacgtca tcaatggcgg cgcaattccg gtgacagtgg tgagtctgca
     1981 gaccaatcgc acctcggtgc gggtcacaac cgctgccgat ctggtctcgg gaacgaacta
     2041 cacgctggtg atcaacggtg tcgaagatgt gtatcacaac ctgagcgcca tcaacaccac
     2101 gttccaggga cgtgatatgg tggctccggt gttctcggta ggcgtcttct cgaacccggc
     2161 aaacgagtat gatctcatcg ttgctgcgac tgtgaacgaa gcacttgcag cgagtccgaa
     2221 atcaaaggtg atgcaaaacg gcgctccatc agttgagtat acgatgactc ctggtacttt
     2281 ggcgaactcg tatatgagtg gcgcccatct cgacccgaac tatcccggag cgggttgggt
     2341 cgagatcagc ggaaccgatg tggccgggaa tctcggaacg acccttgcca gctttaccct
     2401 tgcggtcgtc aatgcctcca tccgggccga ggtgaagtcg tcggatgggc agttcacgag
     2461 tgtgtttgct gccggcagcg taaaacagaa aaccgcggtg aaatggttgc cacgagggaa
     2521 cctcacccag aatgctgccg cgaacgtcct ggttgct
//
LOCUS       DAORHD010000718         2556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48605_length_2556_cov_3.067973, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000718 DAORHD010000000
VERSION     DAORHD010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48605_length_2556_cov_3.067973"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PKO06_14490"
     CDS             <1..890
                     /locus_tag="PKO06_14490"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNV70907.1"
                     /translation="PLLGFFNVKGMNSTEVQPALAKALAEKVFQGPRVQVSLMKQAQS
                     DKVYVFGGVKTPGMLQIRDSVSLMDAVQMAGGFEDGNLYGNNQWLTNPTDPRSNMAGF
                     QVTATFDYLSDTKLKRGGKETKIDLKKLIMDGDVAQNIALQPNDVLLIPRRTAVALKD
                     DETLYILGAVEFPARYRFRDNMSIMDAITMAGGLTNKHHIKFASIIRGIKKDADPKSV
                     KPEVLKIDLRRFYYRGDISSDVALKPGDIVLVSTREYRNFLSQLGKFVDNFVPILGRA
                     DTVVGFDDTIRRFNDRFIK"
     gene            1086..2447
                     /locus_tag="PKO06_14495"
     CDS             1086..2447
                     /locus_tag="PKO06_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HNV70908.1"
                     /translation="MQELEKLLTSYADFVWGPPLLILLLGTHLFLTFRLRFIQRYIGL
                     GIKLSLERKQEGQGDVSQFGALTTALAATIGTGNIIGVGTAISVGGPGAVLWMWLTGV
                     FGIATKYAEALLSVKYRTVNAEGNMAGGPMYVLDKGMNLRWLGVAFAALTAVAAFGIG
                     NMVQANSISSLLEKQFALPTWVSGIVMAVITAAVILGGIKSIARVCELLVPFMAIFYV
                     GGCLFILLKNIGQIPETIRIILSGAFTGQAAVGGFVGAGVLQAMRFGVARGLFSNESG
                     LGSAPIVAASAQTRNPVRQALVSSTGTFWDTVVVCLVTGLVLVNSGDWQTGLKGDELT
                     YAAFRHIPYIGQLVLSIGLLTFVFSTILGWSYYGEKAIEYLFGLGAVKPYRYLWVAFV
                     FVGSISSLKAVWAFADIANGLMAIPNLVSLLVLSSVVVRETQTYLWTENAINNQSSPE
                     PTS"
BASE COUNT          514 a          645 c          740 g          657 t
ORIGIN      
        1 tgcctttgct gggctttttc aatgtgaagg ggatgaacag caccgaagta cagcctgcgt
       61 tggcgaaagc tctggcagaa aaagtgtttc agggccctcg tgtacaagtt tccttgatga
      121 aacaggctca gtcagacaag gtttacgtgt ttggtggcgt gaagactccc ggcatgcttc
      181 agatccggga ttctgtttct cttatggatg ccgtgcagat ggccggtggt ttcgaagatg
      241 gcaacttgta cggaaacaac cagtggttaa ccaacccgac tgatccgcgc tccaacatgg
      301 cgggtttcca ggtcacggca acctttgatt acctgtcaga taccaaactg aaacgtggtg
      361 gcaaagaaac gaagatcgat ctgaaaaagc tcatcatgga cggtgacgta gcgcagaaca
      421 tcgcattgca gccgaacgac gtgctgttga ttccgcgacg cacggccgtc gcgctgaaag
      481 acgacgagac actctatatt ctgggtgctg tcgagtttcc tgcacggtat cggttccgtg
      541 acaatatgtc gatcatggac gccattacga tggccggtgg tttgacgaac aagcatcata
      601 tcaagtttgc cagcatcatt cgcggcatca agaaagatgc agatccgaag tcggtgaaac
      661 cggaagtgct gaagattgat ctgcgtcgct tctattatcg cggagatatc agcagtgatg
      721 tcgcgttgaa gccgggcgac atcgtgctcg tctcgacacg cgagtatcgc aatttcctct
      781 ctcagctggg caagtttgtc gacaactttg tgccgattct gggccgcgcc gatacggtgg
      841 ttggtttcga cgacacgatt cgccggttca acgaccgatt tatcaagtaa actcctgacg
      901 tttctgacgg gggtctgaat ctgattcgga tccccgtttt tcgttcccga ttttcatgcg
      961 ctcgggggcg agaaatgggt ggtttgcctg tcatgtcgtg aatacaacac gcacttccgt
     1021 agtggataag tggatgtcgt tttgccgata acgggaagat cagacacttt atcgcagggg
     1081 gcatcatgca ggaactcgag aaacttctga cttcatacgc cgattttgtg tggggaccac
     1141 cgctactgat cctgcttctc ggcacacatc tgtttctcac ctttcggttg cgcttcattc
     1201 agcgctatat cggccttggg atcaaactat ccctggaacg aaaacaggaa ggtcagggcg
     1261 atgtcagtca gtttggagcg ctgacgacgg ctctcgctgc caccatcgga acgggaaaca
     1321 tcatcggcgt ggggacggcg atcagtgttg gcggtcctgg ggctgtgctc tggatgtggt
     1381 tgactggtgt attcggcatt gccaccaagt atgccgaagc cttgttgtcg gtgaagtatc
     1441 gaaccgtaaa cgctgaaggc aacatggcag gtggccccat gtacgtgctc gacaagggaa
     1501 tgaaccttcg ctggcttggg gtggcttttg ccgcgttgac tgctgtcgcg gccttcggca
     1561 tcggcaatat ggtgcaggcg aactcgattt cgtcgcttct cgagaaacaa ttcgctttac
     1621 cgacctgggt ttctggcatc gtgatggcgg tgattaccgc ggcagtcatt ctgggcggaa
     1681 tcaagtcgat tgcccgcgtc tgcgaactgc tggtgccctt catggctatc ttctacgtgg
     1741 gaggttgcct gttcatcctg ttgaaaaaca ttggtcagat tccggagacg attcgcatta
     1801 ttctcagcgg tgcctttact ggccaggcgg ccgtaggagg attcgtcggc gctggggtct
     1861 tgcaggcgat gcggtttggt gtcgcccgcg gtttgttttc gaatgagtcc gggctgggca
     1921 gtgcgccgat cgttgcggca tcagcgcaga ccaggaaccc ggtgcgacag gcactcgtct
     1981 cgtcgaccgg aaccttctgg gacacggttg ttgtctgtct cgtgaccggc ctggtgctgg
     2041 tgaattcggg cgattggcag acggggctca agggtgatga actgacctat gccgcattca
     2101 gacacattcc ttacatcgga caactcgtac tcagcatcgg actgttgacc ttcgtttttt
     2161 ccacgattct tgggtggtcc tactatggtg agaaggcgat tgagtacctg ttcggccttg
     2221 gagccgtgaa gccgtatcgc tatctctggg ttgcgttcgt atttgtcggc tcgatctctt
     2281 ccctgaaagc ggtctgggct ttcgccgata ttgcgaatgg tctcatggca attcccaacc
     2341 ttgtgtcttt gctggttttg agctcggtgg tggttcgtga gactcaaacc tatctctgga
     2401 ccgagaacgc gatcaacaac caaagttcgc ctgagccgac gtcgtaaggc agacgatcga
     2461 tctttctcgc agagctccca aaaactgttg ggagctctgc tttgtctctc cctcccggca
     2521 cccttcagga ttcgtctgca tgtgtccggc aacccg
//
LOCUS       DAORHD010000719         2551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48782_length_2551_cov_2.873798, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000719 DAORHD010000000
VERSION     DAORHD010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48782_length_2551_cov_2.873798"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2346)
                     /locus_tag="PKO06_14500"
     CDS             complement(<1..2346)
                     /locus_tag="PKO06_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV70909.1"
                     /translation="MSDWHHFTGKPVYVIVFWLGVIAFGVMGYLEVPRDLFPDTMPPQ
                     VAVVTVMRGASAEDVNRLVTSIVDREIKGLTGLKKVTATSKDEISSINAEFEYGKNLG
                     EAVNDVLTAVSRARRQLPVGVEEPQLFRITEANRPLLTLSIRPRDSQQHNIIEVRQVV
                     ENDLKELLLRLPGIGRVDVFGAHEAEVSVRVNHARLREYDLSIDNVVAILAASNLSVP
                     GGYQETARGEVLVRTVAEAQRPSDLARLPIRVVKGGVLTLGDIASISLAMKNPRSLYH
                     GDGEPAVALNVLKPEGGNSLEGILAVKSALPVLERKYPHLQFSVTTDQQPIIDKNFAG
                     MTGSLFSAVWLTMLVVLVLLLEWRTALIVGVSIPLSFLTTFAFLLFSGYTLNMVTLSG
                     LIIAVGMVVDASIVVVENVLRRLQGGEPGPQAVTAGTREVLFSIFGGTLTTVVVLVPL
                     MFTGGYVQQTMRPLSLTISTTLIGSFVAAVTAVPLLLRWFFAGTTRDEAAAESCWHHR
                     LTSPIVALVNGFLDAITEAYLWLLRLALYLRVFVLLASVAAFVLTVNLAMPLVGMELM
                     PRMDTGLLTIRLDLPTALSWRQCREVLQRVEMVLKREPQVRHISSVMGAEPGQISFGA
                     GGQLLQQVEMQVTLTTRDQRTTTIWEIMERWRGELAAIPEIVSAAVIEYGATPVSTTR
                     APIDVMITGRDPRVLDRLGRELLTRLKTIKGLRDLRPNWTCTKLERMYHPHLSVALQA
                     GLSPKRLGELLQVAFSGRVVTKLKMEGFLDLPIRLDLGHESR"
     gene            complement(2343..>2551)
                     /locus_tag="PKO06_14505"
     CDS             complement(2343..>2551)
                     /locus_tag="PKO06_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70910.1"
                     /translation="ATGTVVLAYAVAAGQAQRLRLVPLFLDDDGNAVVEAASFPNGLR
                     LARLPYLQQARWPQRLAVREEDNP"
BASE COUNT          589 a          798 c          706 g          458 t
ORIGIN      
        1 cctgctctcg tgcccgagat caagacgaat gggtagatcg aggaaacctt ccattttgag
       61 tttggtgacg acacggccgg aaaaggccac ctgcagaagt tctcccagac gtttaggcga
      121 cagacctgcc tgcagagcca ccgacagatg cggatgatac atgcgctcga gcttcgtaca
      181 tgtccagttt ggtcgaagat cacgcaagcc tttgatcgtc ttcagacggg tcaggagttc
      241 tcgacccagt ctatcgagaa cgcgcggatc gcggccggtg atcatgacat caataggggc
      301 tcgggtcgtg gaaacgggag tcgccccata ttcgatcacg gcagccgaga cgatctcggg
      361 gattgcggcc agttcacccc gccaccgctc catgatttcc cagatagtgg tagtgcgctg
      421 atcacgcgtc gtcagcgtca cctgcatctc gacctgttgc agaagttggc cacccgcccc
      481 gaaactgatc tgaccgggct cggcccccat gaccgacgag atgtgtcgca cctgcggttc
      541 acgtttcagc accatctcga cccgctgtaa tacctcgcga cactggcgcc aggaaagggc
      601 ggtcggcaga tcgaggcgaa tggtcagcag gccggtatcc atgcgcggca tcaattccat
      661 tcccaccagc ggcatggcca gattcacggt cagcacaaac gcggcgacgc ttgccagcaa
      721 cacgaagaca cgcagataca gcgccaggcg caacagccag agataggctt ccgtaatagc
      781 atcgagaaac ccgttcacca gagccacgat gggagatgtc aggcggtgat gccagcatga
      841 ttcggctgcg gcttcatcgc gagtggtgcc ggcgaaaaac caccgcagca acagcggtac
      901 cgcggtaacc gccgccacaa acgagccgat cagcgtcgtc gagattgtca gagacagggg
      961 gcgcatcgtc tgctgcacgt agcctccggt aaacatgagc ggcacgagaa ccaccaccgt
     1021 ggtcagcgtg ccaccgaaaa tggaaaagag gacttcgcgg gtgccggcag tgaccgcctg
     1081 cggtccgggt tcgcccccct gcaggcgacg cagcacgttc tcgacaacga cgatcgacgc
     1141 atcgaccacc atcccgacag caatgatcaa ccccgaaagc gtcaccatgt tgagcgtata
     1201 cccggagaac agcagaaacg cgaaggtcgt cagaaacgac agaggaatgg agacgcccac
     1261 aatcagagcc gttcgccatt caagtaacag taccagaacc accagcatcg tcagccagac
     1321 cgctgaaaag agtgaaccgg tcatgccggc aaagttttta tcgatgatcg gctgctgatc
     1381 tgtcgtgacc gaaaactgca gatgcgggta tttccgctcc agtaccggca acgcgctctt
     1441 gaccgcgaga attccttcca gcgaatttcc gccttcgggc ttcaacacat tcagtgcaac
     1501 tgccggttca ccatcgccgt gatacagact tcgcgggttc ttcatcgcga gcgagatcga
     1561 ggcaatatca cccaacgtca gcactccacc cttgacaaca cgaatcggca gacgagccag
     1621 gtcagagggg cgttgcgctt cggcgaccgt tcgaaccagc acctcacccc gcgcagtctc
     1681 ctgatacccc ccaggcaccg acagattcga agccgcgagg atggccacca cgttgtcgat
     1741 tgaaagatca tactcgcgca gacgggcatg attcacccgc accgacactt ccgcctcatg
     1801 agccccgaac acgtcaaccc ggccgattcc gggcagccgc agcagcaact ccttcaggtc
     1861 gttttcgacc acctgtctta cttcgattat attgtgttgt tgcgagtcgc gaggtcgtat
     1921 ggaaagggtc aacaggggtc gattcgcctc ggtgatacga aacaattgcg gttcctcgac
     1981 tccgaccggc aattgacgac gcgctcgtga caccgcggtc agcacatcgt tcaccgcctc
     2041 gccaaggttt tttccatact cgaactcggc attgatcgaa gaaatctcat ccttcgaggt
     2101 ggccgtcacc tttttcagtc cggtcagccc cttgatttcg cgatcaacaa ttgacgtgac
     2161 cagtcggttc acgtcttcgg ccgaggctcc gcgcatgacc gtgaccacgg ctacctgcgg
     2221 cggcatggta tcgggaaaca gatcgcgcgg gacttcgaga taccccatga cgccgaaagc
     2281 gatgacgccc aaccagaaca caatgacata gaccggtttt ccggtgaagt ggtgccagtc
     2341 gctcatgggt tgtcttcctc ccgaaccgca agtcgctggg gccaacgagc ctgctgcaga
     2401 tacggcagac gcgcgagacg taacccgttc gggaacgagg cggcttcaac gactgcattt
     2461 ccgtcgtcat ccaggaacag tggaaccagc cgcagacgct gagcctgccc ggcagcaacg
     2521 gcgtaggcca gaacgaccgt accagtcgcc g
//
LOCUS       DAORHD010000720         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_48948_length_2546_cov_3.266158, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000720 DAORHD010000000
VERSION     DAORHD010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48948_length_2546_cov_3.266158"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PKO06_14510"
     CDS             complement(<1..781)
                     /locus_tag="PKO06_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70911.1"
                     /translation="MSWNTRRNILQGIAALVLSFLVPAIGWTQTAFEKQLPAVFRLWN
                     AEKATELALVLQRLGNGGVIEDVRLFLLAECLKRAGHDAEASDLYGRLMKQYPDSIGA
                     RRAGLPYILMMTKNGVSPESLVLLKQLALALPTAYQRGRALEGLAQALPPGHREKGEL
                     ALLSLRAYREPSGSLAPAKDAVNLLKSLIENHGQWSLTPEEWVEICLAATAEGLGQSV
                     LKLGAFLKPTLGPHGAGYQLLIQADALRMLGQRDRALSLLES"
     gene            948..>2546
                     /gene="fusA"
                     /locus_tag="PKO06_14515"
     CDS             948..>2546
                     /gene="fusA"
                     /locus_tag="PKO06_14515"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV70912.1"
                     /translation="MKIYAPESIRNIGLMSHGGAGKTSLAEAILFHTGANSRIGKVDD
                     GSTMLNYDPEEVRRKISIGVSLAAIEWKNTKINLLDAPGFDDFLGEVWKIVPAMDAAI
                     VVVNAGSGIEVGTEKNWKILNTHNIPRIVYLSKLDKENINVQKIIDDLKAFDSKITPI
                     QIPLGTHANLKGVIDVLEMKAYFYQDDQGKKFEVKDIPAEYKDMAAKLREAAVESIVE
                     CDDDLMNKFFEVGADGIAKDELHRALKTGLAQNKIKPLLCGMALKNLGTDQLLDFIIN
                     CMPSPVERGAIEALTANGEKKMIEPKADGPAVGFIFKKASDQAGEMLFMRGVSGKFSG
                     GTEYFNTNKDNTERLANFSTLRGKTKIELDGLGAGDIAIIVKPKITTRGDTLCEKAQQ
                     VKIVIPALPEPRLTYAVNPRTKQDQERMGTGLQTLCGDDGILKYEFDPELGQGLISGL
                     GDTHIDVAICRLKLRWNVEVDIGKPRIPYRETIKGTADVQGKHKKQSGGRGQFGDVTI
                     KFEPCPDKEFEFVDAIVGGVVPRNF"
BASE COUNT          607 a          772 c          661 g          506 t
ORIGIN      
        1 gactttcgag cagtgacaga gcacggtccc gctggcccag cattcgcagt gcatctgcct
       61 gaatcagcaa ctgatacccg gcaccgtggg gtcccagggt cggcttgaga aaagctccca
      121 gtttcagcac tgactgcccc aagccctccg cggtggcagc cagacagatc tcgacccatt
      181 cttcaggtgt cagtgaccac tggccatgat tctcaatcag gctcttcaaa aggttcacgg
      241 catctttggc cggagccagc gagcccgatg gttcgcggta cgcccgcaga gacaacaggg
      301 ccaactcccc cttttcgcga tgcccgggag ggagcgcctg cgccaatcct tcaagcgctc
      361 gcccacgctg ataggcggtc ggcaacgcca gagccagttg cttgagcagc accagggatt
      421 ctggcgacac cccatttttc gtcatcatca ggatatacgg caaacccgct cgccgagctc
      481 cgatcgaatc gggatactgt ttcatcaagc gcccgtagag atcgctcgct tcggcatcgt
      541 ggcccgctcg cttcagacat tccgccagca gaaacagtct gacatcttcg atgaccccac
      601 cgtttccaag tcgctgcaac acgagagcca gttcagtggc cttttccgcg ttccagaggc
      661 gaaagaccgc cggcaactgt ttttcaaagg ctgtctgggt ccatccaatg gcgggaacaa
      721 ggaaactcag caccagtgct gcaattccct gcaggatgtt acgtcgcgta ttccatgaca
      781 tgcgttttct cctcgtgaag gtctcaagaa cttgtaccgt aaacgcgaaa gaggtttcaa
      841 gagggtattt tcctgtactg cgccgcattt tcgttgcatt cccgggcaaa atccgttata
      901 catgtaaccc actatttttc tttccagaac gctttcagga gtcaatcatg aagatctacg
      961 cccccgagtc cattcgtaac atcggcctga tgtctcacgg cggagccggc aaaacctcgc
     1021 tcgccgaggc tatcctgttt cacaccggcg caaacagccg catcggcaag gtcgatgatg
     1081 gcagcaccat gctgaattat gatccagaag aggttcgccg caagatcagc atcggcgtct
     1141 ctctcgccgc catcgaatgg aagaacacca agatcaacct gctcgacgcc cccgggttcg
     1201 atgatttcct cggcgaagtc tggaagatcg taccagccat ggacgccgcc attgtggttg
     1261 tcaacgcagg ttccggcatt gaagtcggta ccgaaaagaa ctggaagatc ctcaacaccc
     1321 acaacattcc tcgcatcgtc tatctgagca aactcgacaa agaaaacatc aacgttcaga
     1381 agatcatcga cgatctcaaa gccttcgatt ccaagatcac cccgattcag atccctctgg
     1441 gaacgcatgc caatctgaag ggtgtcattg atgtgctcga aatgaaggcc tacttctacc
     1501 aggatgacca gggcaagaag ttcgaagtga aggatattcc ggctgagtac aaagacatgg
     1561 ctgccaaact gcgcgaagcc gccgtcgagt cgatcgtcga atgtgatgac gacctgatga
     1621 acaagttctt cgaagtgggt gccgatggca tcgccaaaga cgaactccac cgcgcgctca
     1681 agaccggtct ggcgcagaac aagatcaagc cattgctctg cggcatggcc ctcaagaacc
     1741 tcggcaccga tcagttgctc gatttcatca tcaactgcat gccctcaccg gtcgaacgcg
     1801 gcgccatcga agccttgacg gccaacggcg aaaagaagat gatcgaaccc aaagccgatg
     1861 gccctgccgt cgggttcatc ttcaaaaaag ccagcgacca ggctggtgaa atgctgttca
     1921 tgcgcggtgt ttccggcaaa ttctccggcg gcaccgagta tttcaatacc aacaaggaca
     1981 acaccgagcg tctggcaaac ttctcgacgc tgcgcggcaa aaccaagatc gaactcgatg
     2041 gtctcggcgc cggcgacatc gccatcatcg tgaagccgaa gatcaccacg cgcggtgaca
     2101 ccctgtgtga gaaggcacag caggtcaaga tcgtgattcc ggccctgccc gaacctcgcc
     2161 tgacctatgc cgtgaacccc cgcaccaaac aggaccagga gcgcatgggc accggtctgc
     2221 agactctctg cggtgatgat ggcatcctga agtatgaatt tgatcccgag ttgggtcagg
     2281 gcctgatctc cggcctcggt gacacgcaca tcgacgtcgc catctgccgt ttgaaactgc
     2341 gctggaacgt cgaagtcgat atcggcaaac cccgcattcc gtaccgcgag accatcaaag
     2401 gcaccgcgga cgttcaaggg aaacacaaga agcaatcggg tggccgtggt cagttcggtg
     2461 atgtcacgat caagttcgaa ccctgccccg acaaggaatt cgagtttgtc gacgccatcg
     2521 tcggcggcgt ggttccccgc aacttc
//
LOCUS       DAORHD010000721         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49005_length_2544_cov_3.261551, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000721 DAORHD010000000
VERSION     DAORHD010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49005_length_2544_cov_3.261551"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PKO06_14520"
     CDS             complement(<1..1430)
                     /locus_tag="PKO06_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70913.1"
                     /translation="MRLWKWLSLLGLPLLLVLLPFLMLGFRMTDGQRPLGTPQWQARL
                     EALANRLQRESSFDFWLEESLRRFRRSLRRGLAASDWPLATQVQKARSDARVGTIPEG
                     YCWLVKFPVGTSSTKPQLVDVDGMNSPNGVTIRNLLEQVWLSQVGRESALESKRWATI
                     LKNQFGLFITADMFLPRMRGRAFPVVFQSRYRWMAWDFILRPGKPEPVAAVAMMFDAP
                     TQNVADILIPTVFREWKARDVLPVFLTLPTEPGGARIRRFLHPELHAPLIAPVLAEVQ
                     REMEISLPDDINKTQVGDVRLPFRRCGQNFALGDQVYRVVPLNEFHECLGVLVTKTPP
                     ARQPWAELVPRWYAGALGLVLVLYGFRWWCLGKPPDLSVRGQLLAWLVGLVLAPWLLA
                     MAVGSQFLGDLRENLIDARVRDIEATMAHLDAGIGSLLAASERACRKALEAPGLVDRL
                     KQAQLDGKPTEPILADIVERADQAGL"
     gene            1558..1959
                     /locus_tag="PKO06_14525"
     CDS             1558..1959
                     /locus_tag="PKO06_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70914.1"
                     /translation="MRGLILKNVLFVLLALILVSPAFTHVLKTREGVKLYLKSSVSLK
                     DTWLDLTGLSMPQLRHHPEVVKLLLSKGDERFLPGGAVLRSLYDLGLDMQEALEKLDG
                     EVKVTETLGELKQLGATRARELRQKATKLLE"
     gene            1986..2501
                     /locus_tag="PKO06_14530"
     CDS             1986..2501
                     /locus_tag="PKO06_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70915.1"
                     /translation="MNRSVVSALFGGFLLILFFLISSFQADSADGVSRATGSSSSSGS
                     SYSDAGGSSGGSSSDFDQEAYERRKKRKEREEEEKKKEEEAKKETASEKSDNEDDEEN
                     EADENANPDDISADKSGNASEAATTGSREPPPAAGNPITVLYECPKCKFVSDQSCICP
                     KCKIPLVAPGN"
BASE COUNT          604 a          765 c          710 g          465 t
ORIGIN      
        1 ttcagtccag cctggtcggc acgttcaacg atatcggcga gaatgggttc ggtgggtttt
       61 ccgtcaagtt gcgcctgctt gagacgatcg accaggccgg gtgcctcaag ggctttgcgg
      121 caggcgcgtt cgctggctgc cagaagactg ccgataccgg catcaaggtg agccatggtc
      181 gcttcgatgt cgcgcacgcg ggcatcgatc aggttttcac gcagatcacc cagaaactgc
      241 gaaccgacgg ccatcgccag aagccaggga gccaaaacca acccgaccag ccaggcgagc
      301 aactgacctc gcaccgaaag atctggcggt tttcccaggc accaccagcg aaatccatag
      361 agaaccagaa ccagaccgag agccccggca taccagcgcg gcaccagctc cgcccacggc
      421 tgtcgtgccg gtggagtttt cgtcaccaac actccgaggc actcatgaaa ctcgttgagg
      481 ggaacaacac gatacacctg atcacccaga gcgaagttct gcccgcaccg cctgaatgga
      541 agccgaacgt caccaacctg cgtcttgttg atgtcgtcgg ggagagagat ctccatttcc
      601 ctctgcacct ctgccagtac cggtgcgatc agaggcgcat gcagctccgg atgcaggaaa
      661 cggcgtattc gcgcccctcc cggttcggta ggcagagtca gaaacaccgg caggacatcg
      721 cgagccttcc attctcgaaa cacggttgga atcaggatgt cagcaacatt ctgcgtcggt
      781 gcatcaaaca tcatcgcaac cgctgccacg ggctccggct tccccggtcg cagaatgaaa
      841 tcccacgcca tccatcgata tcgagactga aacaccaccg gaaaagcccg cccccgcatg
      901 cggggcagaa acatgtcggc agtgatgaac agaccaaact ggtttttcag aatcgtcgcc
      961 cagcgcttgc tctccagagc actctcacga ccgacctgtg acagccagac ctgttcgagc
     1021 agattgcgga tcgtcacccc gttcggactg ttcatcccgt cgacatcaac cagttgcggc
     1081 tttgtcgaac tcgtgccgac cgggaacttc accagccagc agtatccttc cggaatcgtg
     1141 ccgaccctgg catctgatcg agctttctga acctgtgtcg ccaatggcca atctgatgct
     1201 gccagaccgc gacgcagaga tcggcggaac cggcgaagag attcttccag ccagaaatcg
     1261 aatgaggact cgcgctgcag gcgattcgcc agggcttcca gacgcgcctg ccactgcggc
     1321 gttcccagtg gcctctgccc atccgtcatg cgaaacccaa gcatgaggaa tggcagcagc
     1381 acaaggagca acggcagtcc caacagtgac agccacttcc acaggcgcat gcaaagccac
     1441 tccacaggca gttttcgact gaacgaacca cgttgaggcc cgttttcgga caatgcctca
     1501 tctccgcatc aatgatgcgg catgcacatt atcggcgaat agagtagaat ggttcacatg
     1561 agaggtttga ttctgaagaa tgtcctcttc gtcctgctgg ccctgattct cgtgtctccg
     1621 gcattcacac acgtgctcaa gacgcgcgaa ggggtgaagc tctacctgaa gtcatccgtg
     1681 tctctgaagg acacatggct ggatctcacc ggactctcga tgcctcagtt acgacaccac
     1741 cccgaggtgg tgaaactgct tctcagcaaa ggggatgagc gcttcctgcc cgggggagcc
     1801 gttttgcgaa gcctgtatga tctcggcctc gacatgcagg aagccctgga gaagctcgac
     1861 ggagaagtga aagtcaccga aacgctgggt gagttgaagc agctcggagc gacccgagcc
     1921 cgggaacttc gacagaaagc gaccaagttg ctcgaatgac gtgtcattga agggggaata
     1981 cattgatgaa ccgttctgtc gtcagcgcac tgttcggagg cttcttgctg atcctgttct
     2041 ttctgatctc atcgtttcag gccgattctg ccgatggggt gagccgggcc acaggcagct
     2101 cgagttcaag cggctcgtcg tattctgatg caggtggttc gtctggcggt tcttcatcgg
     2161 atttcgatca ggaagcttac gaacggcgga aaaagcgcaa agagcgagaa gaagaggaaa
     2221 agaaaaagga agaggaagcc aaaaaggaaa ctgcctcaga gaagtcggat aacgaagatg
     2281 acgaagagaa cgaagcagac gagaatgcga acccggacga catctcggcc gacaagagcg
     2341 gcaacgcctc ggaagccgcg acaaccggct cgcgcgaacc gccaccggca gccgggaatc
     2401 cgatcacggt gctctacgag tgtccgaagt gcaaattcgt cagcgatcag tcctgcatct
     2461 gccccaagtg caagattcca ctggtcgctc cgggaaactg aattctcgcc ccctgccagc
     2521 acgttcgcac ggccggaatc cccc
//
LOCUS       DAORHD010000722         2544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49007_length_2544_cov_2.755323, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000722 DAORHD010000000
VERSION     DAORHD010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49007_length_2544_cov_2.755323"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKO06_14535"
     CDS             <1..252
                     /locus_tag="PKO06_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70916.1"
                     /translation="LLKNITRRMESLENHIKTLEGSLSDARQHMELTMNQRLLLAAPP
                     RPVATWQPEAPPVDDTYLSRFSWWERIFKPERMRRPCLR"
     gene            complement(449..580)
                     /locus_tag="PKO06_14540"
     CDS             complement(449..580)
                     /locus_tag="PKO06_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70917.1"
                     /translation="MKFCWEIYNKSECDTCPYYNSVKEGKTPQDKIPEFMKVDEESN"
     gene            complement(577..927)
                     /locus_tag="PKO06_14545"
     CDS             complement(577..927)
                     /locus_tag="PKO06_14545"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70918.1"
                     /translation="MIETQPFTGSDGQKFYQQGLACDAEGKVQEAIEAYREALRVNPH
                     MAEAYYSLGFDLALVEDRIGAVRMWRKAIWLNADYQRQLISALDLDHELREVEVVPVK
                     TSFSGQEHVKEHRS"
     gene            complement(1057..1668)
                     /locus_tag="PKO06_14550"
     CDS             complement(1057..1668)
                     /locus_tag="PKO06_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV70919.1"
                     /translation="MNRQQRPLDRAGFFRSLRQTLGRLVDEFAGEYLEETAARLEQHF
                     PLLIRPPGALPEESFLKTCRRCGECVRACPHFAIRVYFRSPSFFEKTPYLEPRESFCR
                     MCEDFPCIRACPSGALQRPDKRLPRIGTATVQEALCLNVSHPSATPCRACVDICPGRF
                     SALQAPEVGNRAPRVLVEACSGCGACVSACPARPDPAVCIVPR"
     gene            complement(1665..>2544)
                     /locus_tag="PKO06_14555"
     CDS             complement(1665..>2544)
                     /locus_tag="PKO06_14555"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNV70920.1"
                     /translation="IIPPTNYARHTNIRPLGEDVHAGSPLLEGGTRLDAVALARLASA
                     GITRFLVRRQPRILILTSGNELRRPGCLVPALDRKAAPSNPACPKFECDSTLLRALLA
                     EEGFSARVLPHLPDRRDRFLQTLRGLKPFDLLLTCGGAANSEADHVRPALRELGAEFL
                     FERVRVKPARPTALAHWRRGLVFCLPGNPVAVFAAFHAFVLPTLRAAHGHPDPAGPRF
                     YARLDHDFTSDPRRHLLSRVRVKRTPEGVVASPHPESGSALLRTLIDSNGLAIIEPGD
                     IIPRGTPVPVIWSRSP"
BASE COUNT          556 a          688 c          814 g          486 t
ORIGIN      
        1 ctgttgaaaa acattacgcg tcgcatggaa tcgctggaaa accacatcaa gactcttgag
       61 ggctcgctca gcgatgcccg ccaacacatg gaactgacca tgaatcagcg attacttctg
      121 gctgctccgc cccgtccggt tgcgacctgg cagcctgaag cccccccggt ggatgacacg
      181 tatctgtcac gattcagttg gtgggagcgc attttcaagc cggagcgcat gcgtcgacct
      241 tgtctgcgct gaagaacaca gagaaagcgg gccgattcct gagaaagatc ggcccgcttt
      301 tgcttttggc gtctggggag ctgaggggtg acctctgatc gtgagtgaaa atcggcgttt
      361 gggatcgcgt ggcaatgaga tacaggaaaa ggaaaatccg ccacaactgg tgtggcggac
      421 tcgtttgtcg gtggtgcggt gggggagctt agttgctctc ttcgtccact ttcatgaatt
      481 cagggatttt gtcctgaggg gtctttccct cttttaccga gttgtaatac gggcaggtgt
      541 cgcattcaga cttgttgtaa atttcccagc agaatttcat gatctgtgct ccttcacatg
      601 ttcttggccc gagaaagagg tcttcacggg aaccacttcg acctcgcgca gttcgtgatc
      661 gagatcgagg gcgctgatca actggcgctg ataatcagca ttgagccaga tggccttgcg
      721 ccacatgcga accgcgccta ttcgatcttc gaccagggcg agatcaaaac caaggctgta
      781 ataggcttca gccatatgcg ggttcactcg cagagcttcg cgatacgctt cgatggcttc
      841 ttggactttg ccctcggcgt cacaggcgag accctgctga tagaacttct gcccgtcgga
      901 accggtgaaa ggttgggttt cgatcataaa gatgttgtaa ttgagatcga cccatttcca
      961 cgaaaaccga agggcaaatt gatgctatca caaaaaagtc cgagatgcaa atggatggaa
     1021 atggcaactg gagcgggttg tcgtgaacga ccgagtctaa cgaggcacga tgcataccgc
     1081 cggatccggt ctggcggggc atgccgagac acaggctcca cagccgctac aagcctccac
     1141 cagtactcgg ggggcgcggt tgcccacttc cggcgcctgt aacgccgaga atcgacctgg
     1201 acagatatcg acacaggcac gacacggtgt cgccgatgga tggctcacat tgaggcagag
     1261 ggcttcctgc acggtggcgg tgccgatgcg tggcagcctc ttgtccggac gctgcaacgc
     1321 ccctgaagga caggcgcgaa tacacggaaa gtcttcgcac atacgacaga aggattcgcg
     1381 cggttccaga tagggcgtct tttcgaaaaa cgagggtgac cggaagtaca cacgaatggc
     1441 gaagtgcgga caggcacgca cgcattcccc gcagcggcgg caggtcttca gaaacgattc
     1501 ttcggggaga gcccccggag gtcggatgag cagaggaaag tgttgttcaa ggcgagcggc
     1561 ggtttcctcg agatactctc ccgcgaactc gtcgacaagc cgacccagtg tttgccgtag
     1621 cgagcggaaa aacccggcgc gatcaagtgg acgctgttgt cgattcatgg ggagcgcgac
     1681 cagataaccg gaacaggcgt gccacgggga atgatgtctc ccggttcgat gatcgccaga
     1741 ccatttgaat cgatcagtgt tcgcagcagt gccgacccgg actccgggtg aggactggcg
     1801 acgacgccct caggggttcg tttcacgcgc acacgcgaca gcagatgccg acgtgggtca
     1861 gacgtgaagt catgatcgag gcgggcatag aaccggggac cagctggatc agggtgtccg
     1921 tgggcggcgc gcagagttgg cagcacgaag gcatggaaag ccgcgaagac cgcgaccggg
     1981 tttccgggca ggcagaaaac caggccgcgg cgccagtggg caagtgctgt gggccgagcg
     2041 ggtttgaccc gcacgcgttc gaacagaaac tccgcgccca gctcgcggag cgcgggtcgc
     2101 acgtgatcgg cctcgctgtt tgccgcgcca ccgcaggtca gaagcaggtc gaacggtttg
     2161 agaccgcgaa gggtctgcag aaatcgatcg cgtcgatcgg gaaggtgcgg cagaacccgg
     2221 gcagagaagc cttcttcggc aagcagagcg cgtagaagcg ttgaatcgca ctcgaacttc
     2281 gggcaggcag gattcgaggg tgccgctttt ctgtccaggg cgggaactag acagcccggc
     2341 ctgcgaagct catttccact ggtgagaatg agaatgcgcg gttgacgccg cacgagaaaa
     2401 cgggtaatgc cggccgatgc aagccgtgcc agggccacgg catcgaggcg tgtcccgcct
     2461 tcgagcagcg gtgaccccgc gtgcacatcc tcgccaagcg gccggatgtt ggtgtgccgg
     2521 gcgtagttgg tcggcggaat gatg
//
LOCUS       DAORHD010000723         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49030_length_2543_cov_3.724277, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000723 DAORHD010000000
VERSION     DAORHD010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49030_length_2543_cov_3.724277"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            403..1086
                     /locus_tag="PKO06_14560"
     CDS             403..1086
                     /locus_tag="PKO06_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70921.1"
                     /translation="MKARIVCCLLLLVALALPTDLFAQNQGWIAYEINAQYRGTVKKG
                     FENLGGGMAQYIDLGGGKRQVVMRGCAAHPEKKNTFYSYRLNMGYAIEGSAVKTFSEG
                     DATFQNFDVDEQRNLKNMIILVSMVQTGEVLPLLKGPFTINGSTIEITGQLAKKGKNS
                     ELTLTHKGKPEMEGKFFLDEVAPGKYSISKFRLKRGKLVMSFVSVPTDKLVAEYKAKN
                     PFNQILFAQ"
     gene            complement(1153..2487)
                     /locus_tag="PKO06_14565"
     CDS             complement(1153..2487)
                     /locus_tag="PKO06_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV70922.1"
                     /translation="MKKVKLVAIVVLVLSVVGLGVLGAKKMLQILEERSSRAPRTSAN
                     AVSVSTTETATEVAAASPASVPGSPDVAVTAVPTSSPAEPLPTPFSGDSSSATSAVVP
                     SSAASQAPASASDWREGFVKWVAFAADKGVHVRAGPDVGEKLLFVVGKGTKGTVTDRR
                     NGWSELKWDFNRQTGWVRDDLLMIGPDEVMQSFVRPDGSVIGTYTPELVKEATIKART
                     EATAAGVPPDAAVTTVTASVESDVPAPIVPVASGEKLVAYAAQKGVNVRAGPATRDKL
                     LFLVGKGTKGIVTAKAGNWSQIKWEFNRKSGWVRNDLLIMGPEEVMRNFVAPDGTMIA
                     SFTPDALKKVTKKAQEIAKNISVGVARPAPPSETVKGFTGGKLPEEAIVSQDGAKLRE
                     TPSTKAAVVGRLHKGVSVKIKAMKQIGKYQWFDVDYSNGRKIGWTREDNLQF"
BASE COUNT          535 a          775 c          677 g          556 t
ORIGIN      
        1 atcacctgct tgaggtctgc tccgccttca tcatcgagat agcctttcgc gcgcaccaga
       61 agaactcgat cacgcacttc cgtggagcat gaaaaacgaa cctgagccac aacactctcc
      121 tccccgaata tcgggatcat gctacatcga tttcgtctca cgcgccactg ttcagcggcg
      181 ttcggttcag aaaaagcgca cgatgccaga cccgcttgtc caaccaccca ttctcgccgc
      241 ctgcagctgc gaaacaccga agtacctggc caaccggccg gcttccctct cgcaggggag
      301 ttcacggcgt gtggaacgat atctttcacg attcagcgtt gacgccggcg cacctgcatg
      361 gtatttccgt ttgtacaatt cacaaggagg ctcgtgtacg atatgaaggc tcgcattgtc
      421 tgttgtctgt tgctgctggt ggctcttgct ctgcccaccg atctgtttgc ccagaatcaa
      481 ggctggatcg cgtatgagat caatgcgcag tatcgcggaa ccgtgaaaaa ggggtttgag
      541 aacctcggcg gtggcatggc gcagtacatc gatctcggcg gtggcaagcg acaggtggtc
      601 atgcgcggtt gcgccgccca cccggaaaag aagaacacct tttactcgta tcgtctcaac
      661 atggggtatg ccatcgaagg cagcgctgtc aaaacgttct cagaaggcga cgccacgttt
      721 cagaacttcg acgttgatga acagcgtaat ctcaagaaca tgatcatcct ggtttccatg
      781 gttcagacag gcgaagtgtt gccgctgctc aaagggccgt tcaccatcaa cggttccacc
      841 atcgagatca ccggccagct ggcgaagaaa ggtaagaact ccgaactcac gctgacccac
      901 aaagggaaac ccgagatgga aggcaagttc ttcctcgatg aagtcgcccc cgggaagtat
      961 tcgatcagta agttccgctt gaagcgcggc aaactggtca tgtcgtttgt gtcggttccc
     1021 acggacaaac tggtcgccga atacaaagcc aagaacccct tcaatcagat cctgttcgcg
     1081 cagtaatcgc aacatcagca aaagagcctg ccggggatcc cctcggcagg ctcttttctt
     1141 tcggtcccac gttcagaact gcaggttgtc ttcccttgtc cagccaatct ttcggccgtt
     1201 gctgtagtca acatcaaacc actggtactt gccgatctgt ttcatggcct tgatcttcac
     1261 gctgactcct ttgtgtaaac ggccgacgac cgcagccttc gtgctcgggg tctcgcggag
     1321 tttggctccg tcctggctca cgatcgcctc ttccggcagt ttgccgccgg taaagccttt
     1381 tactgtctca gacggcgggg cggggcgggc cacgccgaca ctgatgttct tagcgatttc
     1441 ctgtgctttc ttggtcactt tcttaagcgc atcgggcgtg aatgaggcga tcatcgtgcc
     1501 atcgggtgcg acgaaatttc gcatcacttc ttccgggccc ataatcagca gatcattacg
     1561 aacccacccg gactttcgat tgaactccca tttgatctgg ctccagtttc cggctttggc
     1621 cgtcacgatt cctttggtgc ccttgccgac cagaaacagc agcttatcac gtgtcgccgg
     1681 gccagcacgc acatttactc ccttttgagc tgcgtaagca acgagttttt ctcctgaagc
     1741 aactgggaca atcggcgcgg gcacatcgga ttcgacagag gccgtgaccg tcgtgacggc
     1801 cgcatctggc gggacaccgg cggctgtcgc ttcggtgcgt gccttgatcg tcgcctcttt
     1861 gaccagttcc ggggtgtagg tgccaatcac cgagccgtca ggccgaacga agctctgcat
     1921 gacttcatcg gggccgatca tcagcagatc gtcacgcacc cagcctgtct gccggttgaa
     1981 atcccacttc agctcgctcc acccattccg acgatcggtg accgttcctt tggttccttt
     2041 tccgacgacg aagagcagct tttcgccaac atctgggcca gcccgaacat ggactccctt
     2101 atccgcggcg aaggcgaccc atttcacaaa accctcacgc cagtcggaag cgcttgccgg
     2161 ggcttgagaa gcagcgctcg acggcacaac ggcactcgtg gccgaggatg aatcaccaga
     2221 aaatggcgtg ggcagcggct cggcggggga acttgtcggc acggccgtga cggcgacatc
     2281 gggtgaacca gggaccgaag cgggagacgc agcggcgacc tcggtcgctg tctccgtcgt
     2341 ggaaaccgag accgcgttgg ctgaggtgcg cggagcacgg gagcttcgct cctcgagaat
     2401 ctgcagcatt ttctttgcac ccagcacacc aagccctaca accgacaaga ccagaacaac
     2461 gatggccacc aacttgactt tcttcatgtt gccccctcga aaaaactgac agcagtctag
     2521 taccctgccc tctgcctgtc aag
//
LOCUS       DAORHD010000724         2543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49037_length_2543_cov_3.326768, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000724 DAORHD010000000
VERSION     DAORHD010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49037_length_2543_cov_3.326768"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="PKO06_14570"
     CDS             complement(<1..596)
                     /locus_tag="PKO06_14570"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV70923.1"
                     /translation="MIEPNPVIRRLSIVFTLQFALLLLFSAGPAYATVIRTATETCPI
                     CDALVQGSDVWSTNSFGGGDLDFCPHARGTNIIPHLIWSCPSCGYSGYRDDFTASATR
                     SLRKEISAWLTANLPPASIPQNAWEGFEVRALLAQHLNESDLAIGRLYLRAAWCCRVV
                     GVIDGRTRDRTVLAEIYNDHVTRLLAKLQDTDAELVKIS"
     gene            770..2020
                     /locus_tag="PKO06_14575"
     CDS             770..2020
                     /locus_tag="PKO06_14575"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV70924.1"
                     /translation="MITDQLSSPVTSTNRRDLALLWFGQLVSQAGDAVTGMATMWLAL
                     DLTGSKTAAGFVAFAQTLPVLLFSLVAGVAVDRLNRRRLLLLSDAARLLLVCLIPLLY
                     QSGRLNALILAAIVFAMSAFSSLFNPARDTLVPLLVDRERYLRVNALMQSSGYLAFFL
                     GLFGAGGLLGFVSITTLFWFDAATFAVSLISIWLMRVPEAEHAAADGRRAGVLDDLCE
                     GLRYVWREDRRLAWLVAITMANNLFIMGPSVVGMPILVREALSGGSREFAWLESTYGI
                     GMLIGTVLVWRLAKVGRKGTQLMLGLAFDGLTYCPLFWVDHFGISPFAAAVTIIFIHS
                     LGIPFIQVVRTSLIHSIVPSRLQGRVFSMIHFSVMGMTSISVAITGMVAEWLDMRTIF
                     LIIGFGAGVCGLIGLARRDLREAD"
     gene            2091..>2543
                     /locus_tag="PKO06_14580"
     CDS             2091..>2543
                     /locus_tag="PKO06_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNV70925.1"
                     /translation="MSPFKPYREDERPAALPVVPKRFDRNARLWGVRGVDRLARSHVM
                     VLGLGGVGSYAVEGLVRSGVGRLTLVDFDDVCVTNVNRQLPALPATVGRSKAELMAER
                     VKAVNPLVQVESVAAFYEAKTAAAMLGRAPDVIVDCIDNVTAKLHLLAS"
BASE COUNT          442 a          709 c          808 g          584 t
ORIGIN      
        1 gagatcttga ccagctcggc gtccgtatcc tgcagtttgg ccagcagccg agtgacgtga
       61 tcgttgtaga tctcggccag cacggtgcga tcccgtgttc ttccgtcgat gactccgacg
      121 acgcgacaac accaggcggc gcgcagatac agacgaccga tagcaagatc tgactcattc
      181 agatgctgtg ccagcagtgc tcgaacctcg aatccttccc acgcattctg cggaattgat
      241 gccggtggaa gattcgcagt cagccaggcg ctgatttcct tgcgcagaga acgtgtcgcg
      301 ctcgccgtga aatcatcacg ataccctgaa tacccacagg aaggacagct ccagatcagg
      361 tgcggaatga tgttggtgcc gcgggcgtgc ggacagaagt cgaggtcgcc cccaccgaag
      421 gaattggtcg accacacatc actgccttgc accagggcgt cacagattgg gcaggtttcg
      481 gtcgccgtgc gtatcacggt cgcgtacgcc ggcccggccg aaaacagcag gagtagcgcg
      541 aactgaaggg tgaaaacgat cgacagacga cgaatgacag ggttcggctc aatcatgggt
      601 ctctccgtga cacgaagatc ggggctggac actcttcagg aagtctacac aaagaagcca
      661 cggttgtctt gccgactgag actgcggaat tgaagaatgc gggggattgt gagatgatgc
      721 gacacccgac gcgtattcgc ggggccattc caggtcattc cgaggttcca tgatcactga
      781 ccagctttcg tcacccgtca cctccaccaa cagacgagat ctggccctgc tgtggttcgg
      841 acagttggtt tcgcaggccg gcgacgccgt taccggtatg gcgacgatgt ggctggcgct
      901 cgatctcacc ggatcgaaga cagcggcggg cttcgtcgcg tttgctcaga cgctgcccgt
      961 gctgttgttc agtctggttg ccggggtcgc tgtcgatcgt ctgaatcgtc gtcgcctgct
     1021 gttgctcagt gatgcggcac gactgcttct ggtgtgtctg attccgctgc tgtaccagtc
     1081 gggacgactc aatgcgttga ttctggcggc gattgtcttt gccatgtctg cgttcagtag
     1141 tttgttcaac cctgctcgcg acaccctggt accgcttctg gtcgaccgtg aacggtatct
     1201 gcgggtcaat gccctgatgc agtcttcggg ctatctggcg ttctttctcg ggctgtttgg
     1261 tgccggaggc ttgctcggct tcgtctcgat cacgacgctg ttctggttcg acgccgcgac
     1321 cttcgccgtt tccctgattt cgatctggct gatgcgcgtt cccgaagctg aacatgccgc
     1381 tgccgatggc aggcgggcgg gcgtgctcga cgatctgtgc gagggtttgc gctacgtgtg
     1441 gcgagaagac cgtcgtctgg cctggctggt cgcgatcacc atggcgaaca acctgttcat
     1501 tatggggccg tccgtggttg gtatgccgat tctggtgcgc gaagcactga gtggcgggag
     1561 tcgcgagttt gcctggctcg aatcgacgta cggcatcggc atgcttatcg gcaccgtgct
     1621 ggtctggcga ctggcgaagg tcggtcgcaa gggaacccaa ctcatgctgg gattggcctt
     1681 cgacgggctg acctactgcc cgctgttctg ggtcgaccac ttcggcattt ctccgttcgc
     1741 ggcggcggtg acgatcatct tcattcacag cctcggtatt cccttcattc aagtggtgcg
     1801 taccagtctg attcacagta ttgtgccttc aagactgcaa ggccgggtct tctcgatgat
     1861 tcatttcagt gtcatgggca tgacctcgat ctcggtggcg atcaccggca tggtcgccga
     1921 gtggctcgac atgcgcacga tttttctgat catcggcttt ggtgccggag tctgcgggct
     1981 gatcggtctg gcccgccgtg acctgcgtga agccgactga gttgcgggca tgaaccgcac
     2041 aggtggttgt ggtatgcttt ggctcgatca cctctctgga aggagcgtct gtgtcaccat
     2101 tcaagccgta tcgtgaagat gaacgtccgg cggcgttgcc ggttgtgccg aaacggttcg
     2161 atcgcaatgc gcgactgtgg ggcgtgcgcg gagtcgatcg tctggcgcgc agtcatgtca
     2221 tggtgctcgg attgggtggc gtaggaagtt atgccgtcga aggactggta cgatcgggcg
     2281 tcggtcgact gactctggtc gactttgacg acgtctgcgt gaccaacgtg aaccgccagt
     2341 tgccggcgtt gcccgcgaca gtcggcaggt cgaaagcgga actgatggcg gaacgcgtga
     2401 aggctgtcaa tccgctggtg caggttgagt cggttgccgc tttctacgag gcgaagaccg
     2461 ccgcggccat gttgggccgc gcgcctgacg tcatcgtcga ctgtatcgac aacgtcacgg
     2521 ccaaactgca tctgctggcg agt
//
LOCUS       DAORHD010000725         2542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49085_length_2542_cov_2.955770, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000725 DAORHD010000000
VERSION     DAORHD010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49085_length_2542_cov_2.955770"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKO06_14585"
     CDS             <1..776
                     /locus_tag="PKO06_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70926.1"
                     /translation="CFFSQNDEMVEALSLYQALFRVRPDFCRLRFSDLLRSEVRCEKA
                     GVCFTLPDATEMGGEPAAKIVIPWEKLAGPDFVWQDEQPRRRYPLRLTSSDGAWELRD
                     FFAGEGFQPNWKLYDHQGNKRPMAWDGWRRCGFSRQGFWALVEGALWTAPQPMFPREH
                     WQRWSWSDIQLVTQPDDHTFCVTLPTRILLVDASGKLLRVLLSHPPSRTRRRQTSFSG
                     AWVVSETLYVSEIFETWGDGEISRLWAVPLPGGRPEAQK"
     gene            complement(721..1377)
                     /locus_tag="PKO06_14590"
     CDS             complement(721..1377)
                     /locus_tag="PKO06_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70927.1"
                     /translation="MKQVLCLLAAGGVLLLIFVLTPPLVMQIAPGFWLSYPPARSWDR
                     LHETLLTMRRTRDHGKRAAAKASLHSLRATLLTYHQDLGRFPHTGSEYTARSIQMADE
                     SCLGLIPNCNVLMNPEVSFPFGFLGKAKTDYQKCWKGPYLDDEPQYAMCDPWDKKIRY
                     TWSNGSLWLQSGGEDTQFDQIEEVIHPAYSGNDLALRIATLTFELPGAHPAGAPPTGD
                     "
     gene            complement(1374..2144)
                     /locus_tag="PKO06_14595"
     CDS             complement(1374..2144)
                     /locus_tag="PKO06_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70928.1"
                     /translation="MKSSHAVGCGLLVIAMGILLVLADQLLPSHVWHSPVGRVVLRVI
                     SLIGAGGSTIPSSTSTSEVARVKAQMTNLKTGLICYQNDLGKPPFVGPTMSAANLERA
                     DTHCLGTTYARNVLVNDTISKPFENLGLPIHLYRKRWKGPYMDSDPAEFMNDPWGKKI
                     VYTLATTTLFLHCGGPDAKRDPLVTAIALGYAGDDITITVMKLKHLSLDTPGAISSST
                     LAVTASAAAEAPVGIEPPTTLLSSEPVVADGTASPTNR"
     gene            complement(2250..>2542)
                     /locus_tag="PKO06_14600"
     CDS             complement(2250..>2542)
                     /locus_tag="PKO06_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70929.1"
                     /translation="FTVDGVADAPTPQEKLIKACVAYNAGPYSTFVKRSWDKLKMGKN
                     YESIGYGLKLKMCLGFELTATEKTLVTKIFDCKLSKVDALVNEYYSNANGLF"
BASE COUNT          510 a          668 c          803 g          561 t
ORIGIN      
        1 tctgcttttt ttcacagaac gacgagatgg tcgaggctct ctcgctctat caagccctgt
       61 tccgggtgcg ccctgacttc tgtcggttga ggttctcgga ccttttgcgt tccgaagtcc
      121 ggtgcgagaa ggcgggcgtc tgtttcacgc tgcctgatgc cacggaaatg ggcggagaac
      181 cagctgccaa gatcgtcatt ccgtgggaaa agctcgcggg acctgatttt gtctggcagg
      241 atgagcaacc tcgccggcgt tatccgctac gcctgacatc gtctgatggg gcctgggaac
      301 tcagagattt ctttgctggg gaaggttttc aaccgaactg gaaactctat gaccatcagg
      361 gaaacaagcg accgatggcg tgggatgggt ggcgacggtg tggattcagc cgccagggtt
      421 tctgggcact ggtggagggg gccttgtgga cggctccgca gccgatgttt cccagggaac
      481 attggcaacg atggtcttgg agcgacattc aactggtgac ccaacccgat gaccacacct
      541 tctgtgtgac tctgccgacc aggattctgc tggttgatgc gtcaggaaaa ttgctgcggg
      601 tgctgctgtc acaccctccg agccggacca gaagacgtca gacgagcttc tcaggggcgt
      661 gggtcgtaag cgagacgctg tatgtcagcg agatctttga gacctggggt gacggtgaga
      721 tcagtcgcct gtgggcggtg cccctgccgg gtgggcgccc ggaagctcaa aagtgagagt
      781 tgcgatccgc aaagccagat cgtttccgga atacgcgggg tgaatgacct cttcgatctg
      841 gtcgaactgc gtgtcttctc cccctgattg caaccagagg ctgccgttcg accaggtgta
      901 gcggatcttc ttgtcccagg gatcgcacat cgcgtattgg ggctcgtcgt cgaggtaagg
      961 tcctttccag cacttctgat agtctgtttt tgctttaccg agaaacccga atggaaacga
     1021 aacctctgga ttcatcagga cattgcagtt cggaatcagg ccaagacagg attcatcagc
     1081 catctgaatc gagcgagccg tgtattcaga cccggtgtga ggaaatcgac cgagatcctg
     1141 gtgatacgtg agcagcgtgg ctctcagact gtgcaaagag gctttggcag cagcgcgctt
     1201 tccatgatct cgggttcgcc gcattgttaa cagcgtttca tgcaggcggt cccacgagcg
     1261 tgccggcgga taagacagcc agaaccccgg tgcaatctgc atgaccagcg gtggagtgag
     1321 aacaaagatc agcagcagca caccgccggc agccagaagg cacagcacct gtttcatcga
     1381 ttggtcgggg aggcggtgcc gtcggcgacg acaggctccg aagagagaag cgtcgtggga
     1441 ggctcgatac caactggtgc ttctgcagcg gcagacgcag tgactgccag tgtcgacgaa
     1501 gagattgctc ccggggtgtc gagactgaga tgcttcaact tcatcaccgt gatggtgatg
     1561 tcgtcacctg cgtagcccaa ggcgattgcg gtcacgagtg ggtcacgctt cgcgtccggt
     1621 ccgccgcaat gcagaaacag cgtggtggtg gccagcgtat agacgatctt tttcccccag
     1681 gggtcgttca tgaattcggc tgggtcagag tccatgtatg gccccttcca gcgttttctg
     1741 tagaggtgga tgggcagtcc gaggttttcg aatggtttgc tgatggtgtc gttgaccagc
     1801 acattgcggg cgtaggtggt gccgaggcag tgggtgtcgg cgcgttcgag gttggcggca
     1861 gacatggtcg ggccgacgaa tggcggtttt ccgagatcgt tctggtagca gatcaggccc
     1921 gtcttcagat tcgtcatctg ggccttgacg cgggcgacct cagacgtcga cgtggagctt
     1981 ggaatcgttg agccccctgc tccgataagg gaaatcaccc gcagaaccac tcttcccacc
     2041 ggtgaatgcc acacgtggga aggcagcaac tggtcggcaa gcaccagcag aatgcccatc
     2101 gcaatcacca gcagaccgca accgacggca tgcgagcttt tcatggtgaa tgcgggtgag
     2161 agcgttgcag cgtcagagcc gtgaggctct gatacatcat ctcaggcctg ctgagctttt
     2221 ccgcgccagg cgggtagccg aagaggctgt taaaacagtc cgttcgcgtt ggagtaatac
     2281 tcattgacga gcgcgtcgac cttgctgagt ttgcagtcga agatcttggt gaccagagtt
     2341 ttttcggtgg ccgtaagttc aaagcccagg cacatcttga gcttgagacc gtagccgatc
     2401 gactcgtagt tctttcccat cttcaacttg tcccaggagc gcttgacgaa ggtcgaatac
     2461 gggccggcgt tataagcgac gcaggccttg atcagctttt cctggggtgt gggagcgtcc
     2521 gccacgccat cgaccgtgaa ga
//
LOCUS       DAORHD010000726         2540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49147_length_2540_cov_3.422535, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000726 DAORHD010000000
VERSION     DAORHD010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49147_length_2540_cov_3.422535"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="xseA"
                     /locus_tag="PKO06_14605"
     CDS             <1..1049
                     /gene="xseA"
                     /locus_tag="PKO06_14605"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV70930.1"
                     /translation="LGALFEAFEKLKARLQQEGLFDPARKVPLPYLPRGVGIVTSAKG
                     AVVQDIFRVIRRRYPNMPLYLVPVKVQGDGAATEIVAGIRRLDTDPRVDVIIVGRGGG
                     SLEDLWAFNEEPVARAIAAAVKPVISAVGHETDTTIADFVADRRAATPSQAGEFAVPV
                     KDDLVRRIVEMYQRLKRNLEHRLEVARQRLARAQACRFLAKPELLIAERRNTIANLIR
                     DVETWYKELVTRARHQYELLAGRLKSLSPRQLISERRNDLMKLIQSLHERYKRNLVAA
                     RHAFEMRQGRLALLNPRALLQRGYILAQDGEGRVVTSIQALACEQIVTLHLRDGRAET
                     RVTRIEPETEEPTP"
     gene            1046..1342
                     /gene="xseB"
                     /locus_tag="PKO06_14610"
     CDS             1046..1342
                     /gene="xseB"
                     /locus_tag="PKO06_14610"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNV70931.1"
                     /translation="MSRTTLTPEMLQELAALDSSAIDAMNYETAMSRLEEVVGALERE
                     GTPLDVGMKLYEVGTLLSRRCSGILDATEARMLQLIGDGTTARETPFDPDKDGR"
     gene            1342..2292
                     /locus_tag="PKO06_14615"
     CDS             1342..2292
                     /locus_tag="PKO06_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV70932.1"
                     /translation="MTLPKQSSSSPAVTSEKKQTFRGLESYLADRQKLVDSALDQWLL
                     PSSTVPQRLHEAMRYSVCAGGKRIRPILCLAVAEGLGASGDAVCKAAAALELMHTSSL
                     IHDDLPALDNDDLRRGRPTSHKVFGEAMAIMAGDALIMLSFQWLADLVLMGVPAEQAI
                     ETLRILTSAVGNQGMVAGQVLDLQHDQRSIDLETLQQIHRWKTGALLRAAVEIGAVMG
                     GAGTEERQLLARFGDKIGLLFQIVDDILDVVGTAESLGKTPGKDQREQKATYPNLLGL
                     DEARQRAQTAKSEALQLLAELPRPIPRLRELTEYIHHRVN"
     gene            2295..>2540
                     /locus_tag="PKO06_14620"
     CDS             2295..>2540
                     /locus_tag="PKO06_14620"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNV70933.1"
                     /translation="MNDQLSVVIVAGGMGSRMQNPVPKQFLPLLGKPVLRWSVDCFAA
                     LPMVSELVVVLPPEFQVEGSQILHDVHTSIPLKIVEGG"
BASE COUNT          499 a          715 c          809 g          517 t
ORIGIN      
        1 gccttggggc actctttgaa gcgttcgaaa agctcaaggc tcgattgcag caggaagggt
       61 tgttcgatcc ggcccgcaag gttccgttgc cgtatctgcc gcgcggcgtc ggcatcgtga
      121 cctcggccaa aggggccgtc gttcaggata tcttccgcgt cattcgtcgc cgatatccga
      181 acatgcccct gtatctggtg cccgtgaaag tacagggtga cggagccgcg accgagatcg
      241 tcgccggtat tcggcgcctc gataccgatc cacgcgtcga cgtgattatt gttggacgcg
      301 ggggcggcag tctcgaagat ctgtgggcct tcaacgaaga gcccgtcgcc cgggcgatcg
      361 ccgcggccgt gaagcccgtc atttccgccg tgggtcatga gaccgatacg acgatcgccg
      421 atttcgtcgc cgatcgtcgg gcggcgactc cctcgcaggc cggtgagttt gccgttcccg
      481 tcaaggatga tctggttcgt cgcattgtcg aaatgtacca gcgcctgaag cgcaatctgg
      541 aacaccggct ggaagtggcg cgacagcgat tggcacgggc gcaggcgtgt cgcttcctgg
      601 ccaagcctga acttctgatt gctgaacgcc gaaatacgat tgccaacctg attcgcgatg
      661 tcgagacgtg gtacaaggaa ctggtgacgc gagcccgtca tcagtatgaa ctgctcgccg
      721 gtcgactgaa gtcgctgagc ccgaggcagc tgatatctga gcggcggaat gatctgatga
      781 agctcattca aagcctgcat gaacggtaca aacgtaattt ggtcgctgcc cgacatgctt
      841 tcgaaatgcg ccagggtcgc ctggcattgt tgaacccccg tgctttgctg cagcgcggct
      901 atatcctggc acaggatggc gagggccgcg tggtgacaag tattcaggct ctggcctgcg
      961 aacagattgt gactctgcat ctgcgtgacg gtcgggccga aacccgcgtg acgcgcatcg
     1021 aacctgaaac cgaggagcca accccatgag ccgaacgacg ctgacccccg aaatgctgca
     1081 ggaacttgcc gcgctagact cgtccgcgat cgacgcgatg aactacgaaa ccgcgatgtc
     1141 gcgcctcgaa gaggttgtcg gtgcgctcga acgcgaggga acaccgttgg atgtgggcat
     1201 gaagttgtac gaggtgggca cgctgctcag ccgccgctgt tccggaattc ttgacgcgac
     1261 cgaggcacgg atgctgcagt tgatcgggga tggaacgact gcccgtgaaa ctccgtttga
     1321 tcccgacaag gatggccgct gatgacgctc ccgaagcaat cgtcgtcttc ccctgcggtg
     1381 acttccgaaa agaagcagac cttccgtggt ctcgagtcct atctcgctga tcggcagaag
     1441 cttgtcgaca gcgcgcttga ccaatggtta ctgcccagtt cgacggtgcc gcagcgactg
     1501 cacgaagcga tgcgctacag tgtctgcgct ggtgggaagc gcatacgacc gattctctgc
     1561 ctggctgtcg ccgaggggtt gggcgccagt ggggacgcgg tgtgcaaggc ggccgccgcg
     1621 cttgaattga tgcacacctc atcgttgatc catgacgacc tgccggcgct cgataacgac
     1681 gatctgcgtc gcggcagacc gaccagtcac aaggtctttg gtgaagccat ggccattatg
     1741 gcaggcgatg cgttgatcat gctgtcattt cagtggctgg ctgacttggt gttgatgggt
     1801 gtcccagctg agcaggcgat agagactctt cgcatactga cctccgcggt cggaaatcag
     1861 ggaatggtcg ccggacaggt tctcgacctg cagcacgacc agcgctcgat cgacctcgag
     1921 acgttgcaac agattcatcg atggaagacc ggcgctctgc tgcgcgcggc agttgaaatc
     1981 ggcgcggtca tgggcggcgc gggtaccgaa gagcgtcaac tcctggcacg ttttggggac
     2041 aagatcgggc tgctgtttca gatcgtggat gacattctcg atgtcgtagg caccgccgag
     2101 tcgctgggca agaccccggg caaggaccag cgagaacaga aagcaacgta tccaaatctg
     2161 cttgggctcg atgaagccag acagcgtgcg cagaccgcga agagcgaagc gttgcagctg
     2221 cttgccgagc tgccacgacc gattccgcga ctgcgcgagc tgaccgaata catacatcat
     2281 cgggtgaact gactatgaac gatcaacttt ccgtggtcat tgtggcgggc ggtatgggca
     2341 gtcgcatgca gaacccggtt cccaaacagt ttctgccgct gttgggcaag ccggtgctgc
     2401 gctggtctgt cgattgtttc gcggcgttgc cgatggtcag cgaactggtg gtggtattgc
     2461 cgccggagtt tcaggtcgag ggcagtcaga ttttgcatga tgtccatacc tcgattccgc
     2521 tgaagatcgt cgaaggcggt
//
LOCUS       DAORHD010000727         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49344_length_2534_cov_2.961678, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000727 DAORHD010000000
VERSION     DAORHD010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49344_length_2534_cov_2.961678"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PKO06_14625"
     CDS             <1..470
                     /locus_tag="PKO06_14625"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNV70934.1"
                     /translation="YDRAIMHEVVPFIQNRQGGPQGIIASGCSLGAYHAVNFALRHPD
                     VFWATVALSGVFTVKHMIGDYLGSHAYLNSPLDYLPNLNDGWFLDRLRQNRLIVCSGQ
                     GAWEEPGLTDTRRLQQLFAEKGIGHWVDIWGHDVNHDWPWWLRQMPHFLGHLV"
     gene            483..1244
                     /locus_tag="PKO06_14630"
     CDS             483..1244
                     /locus_tag="PKO06_14630"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV70935.1"
                     /translation="MDVPFVDTDERLDTLVGTDLKLIQKIDGTAFAIDTLLLASFVEL
                     PPGGAKVADLGSGSGVLSFMLHYRKPSLEITGFEVLEELHGLSQRNLQLNPNVKNLSF
                     ECCDVRDIPARHFPESFDLVVSNPPYYPKGIGLLPPNPARAAARHEINGTLTDFLEAA
                     AYLLTYGSKLVMIIHTSRFNEAAKTLKDLNFGMRRLRFIHPKEGQNAHLALIEAERFY
                     NGPHEPVPSLTIHQADGKICAEAAEIMSLGLKRSR"
     gene            complement(1266..1916)
                     /gene="nth"
                     /locus_tag="PKO06_14635"
     CDS             complement(1266..1916)
                     /gene="nth"
                     /locus_tag="PKO06_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV70936.1"
                     /translation="MTPRSGSPTSPHRVSALIERLQQVYPGATCALTHHNPLELLMAT
                     ILSAQCTDERVNLVTPTLFKRFPTASDYASANIAELEELVRSTGFYKNKAKSILGCAK
                     QIVAKHGGKVPQTLDELTALPGVGRKTANVVLGTAFGIPSGIVVDTHVRRLSNRLGLT
                     TQDDPEKIEQDLLKIIPEKHWIEFSHLLILHGRNRCSARKPDCPGCEVEDLCPKKL"
     gene            complement(1913..>2534)
                     /locus_tag="PKO06_14640"
     CDS             complement(1913..>2534)
                     /locus_tag="PKO06_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV70937.1"
                     /translation="GITYHSRASGYVNGLSIERVQEQWDQIKNVNSRHPDVRLLRGIE
                     CDLLPDGSLDYPAEVLEGFDFVLVSAHSHFALSREDMTARFLKAVANPFTDILGHPTG
                     RILLERDEYALDLDAILQACIKHHVVVEINANPYRLDLDWRKVRQYRDSGLMFCITPD
                     AHNPEGLDDLRYGLDVARKGGLEKQHLINTLTADEFLHRIRSRRKP"
BASE COUNT          502 a          696 c          760 g          576 t
ORIGIN      
        1 actatgatcg tgcaatcatg cacgaagtcg taccgttcat tcaaaatcgt cagggtggcc
       61 cgcaggggat catcgcctcc ggttgcagcc tgggagcgta tcacgccgtc aatttcgctt
      121 tgcgtcaccc cgacgtcttc tgggcgaccg ttgcgctttc cggtgtcttt acggtgaaac
      181 atatgatcgg tgactacctg ggttcgcatg cctatctcaa ctctccactc gattatctcc
      241 cgaatctgaa cgacggatgg tttctcgatc gactgcgcca gaatcgcctc atcgtctgct
      301 cggggcaggg tgcctgggaa gaacccggcc tgaccgacac acgccggtta caacagctgt
      361 ttgcggaaaa ggggatcggg cactgggtcg atatctgggg gcatgacgtg aatcacgatt
      421 ggccgtggtg gttacgtcag atgccacatt ttctcggtca tctcgtctga cgttttctta
      481 acgtggatgt cccattcgtc gataccgatg agcggcttga cacgcttgtc ggcaccgatc
      541 tgaaactgat tcagaaaatc gacggcacgg catttgccat cgacacgctg ctgctggcga
      601 gcttcgttga gctgccgccg gggggggcga aagtcgccga tctcggttcg ggcagcggcg
      661 ttctgtcgtt catgctgcat taccgcaagc ccagcctcga gattaccggt ttcgaggtgc
      721 tcgaggagtt gcatggtctg tcgcagcgca atctgcagct gaacccgaac gtcaagaatc
      781 tcagctttga gtgttgtgat gtgcgcgaca ttccagctcg ccactttcct gagtcattcg
      841 atctggtcgt cagtaatcct ccctattacc ccaagggtat cggcctgctg ccgccaaacc
      901 cggctcgtgc ggcggcgcgc cacgagatca acggcacgct caccgacttt ctcgaagcgg
      961 cggcctacct gctgacgtac gggtcgaagc tggtgatgat cattcatacg agccgcttca
     1021 acgaggcggc caaaacactc aaggacctga acttcggcat gcgtcgtctc cgcttcattc
     1081 acccgaagga aggacagaac gcgcatctgg cactcatcga agccgagcgg ttctacaacg
     1141 ggccgcacga gccggtgccg tctctgacca tccaccaggc agatggtaaa atctgtgctg
     1201 aagcggccga gatcatgagc cttggtctga agcgatctcg ctagaggttg tgtgagcgat
     1261 ctcgtttaaa gctttttggg gcaaagatct tcgacttcgc agcctggaca atcgggtttt
     1321 ctggctgagc agcgattgcg gccgtgcagg atcagcaggt gggaaaactc gatccagtgc
     1381 ttttcaggaa tgatcttcag caggtcctgt tcgatctttt ccggatcgtc ctgcgtggtc
     1441 agcccgagac gattcgacag gcgacgcacg tgggtgtcaa ccacgattcc tgagggaatg
     1501 ccgaatgcgg tgccgagaac gacgttcgca gtcttgcgac cgacacctgg caacgcggtc
     1561 agctcatcca gtgtctgcgg cactttcccg ccgtgcttgg cgacgatctg tttcgcgcag
     1621 ccgagaatac tcttggcttt gttcttgtag aatccggtcg atcgtacgag ttcctcgagt
     1681 tctgcaatgt tcgctgaagc gtaatcggag gcggtcggaa agcgtttgaa caatgtgggg
     1741 gtgaccagat tgacgcgttc atcggtgcat tgcgctgaca gaatcgtggc catcagtagt
     1801 tcgagcgggt tgtggtgcgt cagagcgcag gtcgcacccg ggtacacctg ttgcaatcgc
     1861 tcgatcaggg cagagacacg atggggcgag gttggtgaac cggaacgtgg ggtcatggtt
     1921 tcctccgtga gcgaatgcga tgcagaaact cgtcggcggt caacgtgttg atcaggtgct
     1981 gtttttcgag cccgcctttt cgggcgacgt cgaggccgta gcgcaggtcg tcgagacctt
     2041 cggggttgtg cgcgtcaggt gtgatgcaga acatgaggcc actgtctcga tactggcgca
     2101 ccttgcgcca atcaaggtcg aggcggtacg ggttggcgtt gatctcaacc acgacgtgat
     2161 gcttgataca ggcttgtaga attgcgtcga ggtcgagcgc atactcgtcg cgctcgagca
     2221 gaatgcgacc ggtcggatgt ccgaggatat cggtgaacgg attggccacc gccttcagaa
     2281 accgagctgt catgtcttcg cgtgacaggg cgaagtgtga gtgcgccgac accagcacga
     2341 agtcaaatcc ctcgagcacc tcggcggggt aatcgagcga accgtcgggc aacagatcac
     2401 actcgattcc acgcagcagg cgaacgtccg ggtgccgact gttgacattt ttgatttgat
     2461 cccactgttc ctgcacgcgt tcgatcgaca gtccgttcac atacccagaa gcacgtgaat
     2521 ggtaggtaat gccg
//
LOCUS       DAORHD010000728         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49465_length_2530_cov_2.746263, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000728 DAORHD010000000
VERSION     DAORHD010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49465_length_2530_cov_2.746263"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKO06_14645"
     CDS             complement(<1..222)
                     /locus_tag="PKO06_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70938.1"
                     /translation="MMNLMKRQRKVLSILLAAFFLGATLGAQAGAPASGAKASTSAKT
                     TAKGSSGSVFSALSPKNLLITTGVAVGTKV"
     gene            437..1495
                     /locus_tag="PKO06_14650"
     CDS             437..1495
                     /locus_tag="PKO06_14650"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HNV70939.1"
                     /translation="MPHNDAHRTLFRYFAILCCLLAVSVPGIGAAGSPCFQTVFNTQT
                     DGHDPVNAYLLMLACTYSYPNRINTATFEEFTAKYKARFSEWGMNTFDFIDVRQKTAD
                     TQVMIMSNERLVIVVFRGSESSSGGSFSPVKAIYDWILTDLNFFPKPVEYWGRKVRVH
                     RGFWNALDVVYPRLKTMVNDHLAGSDKRLWITGHSLGAGIVPLAAYRLAFDGVPVYGV
                     FSFAGPRVGNEEFTDLCRDRFPQFQRWVNDHDLVTMLPFPWLGFKHVSKPNNLYNDGR
                     AVIGDRAFNGLGKVASHPPGPYLQKLYALLPPALRLQVPAPSSLEVTDSASDPGLEKE
                     FRIRRWAHAFFNPPEEED"
     gene            complement(1540..2268)
                     /locus_tag="PKO06_14655"
     CDS             complement(1540..2268)
                     /locus_tag="PKO06_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70940.1"
                     /translation="MKHWIHAVLLTVLVWSALLSPASGADLDPQYLKIFGAGSLIPSG
                     KGLPKKDQYDATQLDVREGAFFIPGGFATATLDARVILPEGETYGTLVEKLKKENPAG
                     AQSQGEEAVWLVWFSHDLDGNRKDQNWPASASSAVLPPSAPDAIFHSCLEERMLSFNT
                     IGLSWVQLCSASSIADLLARAQPGDRIFLAHTVGFLHPPVKTPEATPTSSLQVMEAGQ
                     HNPDLALAIANPRVAVIITVSEPR"
BASE COUNT          535 a          769 c          690 g          536 t
ORIGIN      
        1 aaccttggtg cctacagcaa caccagtcgt gatcagcaga ttcttgggtg aaagtgcgct
       61 gaatactgac ccggacgagc cttttgcagt cgtcttggcc gaagtcgatg ctttggcacc
      121 cgaagccggg gcccctgcct gcgccccaag cgttgcgccc aggaagaacg ccgccagcag
      181 gatcgacaga acttttcgtt gccgtttcat caggttcatc atggttccta cctcctcgcc
      241 tgttgcaggc gtttattatc aaccgatgcg gtacacgatc tttactatta tacaggcgaa
      301 accgggggtc cgggggagtt ttcccaactt tttccgtctg cgacccctgg aagcagtacc
      361 ccaacttcac aataatttac gcgcatgcga acacgaattg agatataata agggtcagat
      421 tggagatcgt cccacaatgc ctcacaacga cgctcatcgc accctattcc gttatttcgc
      481 catcctctgc tgccttctcg ccgtctctgt accaggcata ggagcagccg gaagcccgtg
      541 ttttcagact gtattcaaca cccagaccga tgggcacgac cccgtcaacg cctatcttct
      601 gatgctggcc tgcacctatt cataccccaa ccgcatcaat actgcgacgt tcgaggagtt
      661 taccgcgaaa tacaaggctc gtttcagcga gtggggcatg aacaccttcg acttcatcga
      721 tgtgcggcag aaaaccgctg acactcaggt gatgatcatg tcgaatgaac gcctggtcat
      781 cgtcgtcttt cgcggctcag agagttcatc aggtggttcg ttcagcccgg tgaaggcaat
      841 ctatgactgg attctgaccg atttgaactt ctttccgaaa ccggtggaat actggggtcg
      901 caaagtccgt gttcatcgcg ggttctggaa tgcactcgat gtcgtgtatc cgcgtctgaa
      961 gacgatggtc aacgatcacc tggccggaag cgacaagcgg ctgtggatca ccggtcacag
     1021 cctgggggcc gggatcgttc cactggccgc ctatcgactg gctttcgacg gcgtacccgt
     1081 ctacggcgtg ttcagttttg ccgggccacg agtcggaaac gaagaattca ccgatctctg
     1141 ccgcgatcgc tttccgcagt ttcagcgctg ggtgaacgac cacgatctcg tcaccatgct
     1201 gccgttcccc tggcttggtt tcaagcacgt ttcgaaaccc aacaacctgt acaatgatgg
     1261 tcgtgccgtt atcggagacc gcgcgttcaa cggtctcggc aaagtggcat cccatccccc
     1321 gggaccgtat ctgcagaagc tgtacgcgtt gctgcccccg gccctgcgac tgcaggttcc
     1381 ggcgccatca tcgctcgaag tgacggatag cgcttcagat ccgggtctcg aaaaagagtt
     1441 tcggattcgc cggtgggcgc acgctttttt caaccctcca gaagaagagg actgacgggg
     1501 tttcccccag cgccgaggcg gtggttgagt ccgaagaact cagcgtggtt cgctgaccgt
     1561 gatgatgacg gccacccgcg gattcgcgat ggccagcgcc agatcagggt tatgctgccc
     1621 cgcttccatg acctgtaacg aacttgtcgg cgttgcctca ggtgtcttca ccggtgggtg
     1681 cagaaagcca accgtgtggg ccagaaagat acggtcgcca ggttgcgcgc gtgccagcaa
     1741 gtcggcaatg ctcgaagcgc tgcacagctg aacccaggac aagccgatcg tattgaaact
     1801 cagcattcgc tcttccaggc acgagtgaaa gatcgcatcg ggagcactcg gcggcagaac
     1861 ggcgctcgaa gcagacgccg gccagttctg gtctttgcgg ttaccgtcga gatcatggga
     1921 aaaccagacc agccacaccg cctcctcccc ctgcgactgg gctccggcgg ggttctcctt
     1981 cttgagcttc tccaccagcg ttccgtaggt ttccccctca ggcaggatca cccgcgcatc
     2041 cagcgttgcc gtcgcgaagc cgcccggaat aaagaaagcg ccctcgcgca catcaagctg
     2101 ggttgcatca tactgatctt tcttcggcaa ccccttgccg gaggggatca gcgaccccgc
     2161 tccgaagatc ttcagatatt gcgggtccag atcggctcca gaggccggag acaggagagc
     2221 ggaccacacg agcacagtca acagaactgc atgaatccag tgtttcacaa gttgatccct
     2281 tcaatgactt caggttcatt cggaaaaagg catactacca cacacgccag cgtcatgagg
     2341 cattcttcgc tcatgaaagc ggagcaacta gaagggcgcc aaggcatcat ggtctcggct
     2401 cggtggccgc cgtctctctt tccgcctcgc gctggcgttg agcccgcccg agcaccatcg
     2461 acaacttgag ccagagaacc gcgaccgcaa taccgatcag cggaatcgcc cggtcagcga
     2521 cggatgtcga
//
LOCUS       DAORHD010000729         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49557_length_2527_cov_2.965210, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000729 DAORHD010000000
VERSION     DAORHD010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49557_length_2527_cov_2.965210"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..905)
                     /locus_tag="PKO06_14660"
     CDS             complement(42..905)
                     /locus_tag="PKO06_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV70941.1"
                     /translation="MTRTLVTGATGFIGRHLVPQLLDAGQEVIACGTEPAEQLAFPWL
                     KRVQYVSCDINEPRDDWFERLHRPTRLVHLAWPGLPNYRELFHIEKNLPAAYAFIKSF
                     VQDGVSDITVTGTCFEYGKQEGELSEDSPALPVTAYAMAKDALRRFLEVLRSHCPFSL
                     KWVRLFYLYGEGQAAGSILAQLRSAIQKRESEFRMSGGEQLRDYLPVEIVAQYLSRIA
                     TAPNFSGIVNCCSGAPISIRRLVEQHVRAANSDIRLHLGYYPYSDYQPMAFWGSVKRL
                     HSLFPPPENPT"
     gene            complement(902..1432)
                     /gene="rfbC"
                     /locus_tag="PKO06_14665"
     CDS             complement(902..1432)
                     /gene="rfbC"
                     /locus_tag="PKO06_14665"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNV70942.1"
                     /translation="MKFISTPLQDAYLIEIDAISDERGFFARSWCAREFEKHGLNCRL
                     SQSNLSFNHKSGTLRGMHFQRAPHAEVKLVRCTRGRIFDAIVDIRPSSPTCGKWFGAE
                     LSADNHRMMYVPEGFAHGYLTREDNTEVFYQVSEFYSPESEGGIRFDDPQIGIQWPDP
                     ILLVSPKDQALGYFNR"
     gene            complement(1457..2482)
                     /gene="rfbG"
                     /locus_tag="PKO06_14670"
                     /pseudo
     CDS             complement(1457..2482)
                     /gene="rfbG"
                     /locus_tag="PKO06_14670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02622.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
BASE COUNT          612 a          635 c          723 g          557 t
ORIGIN      
        1 cctgagggct tcgggttggc gcaggtcggc aggagattgt gtcatgtggg gttctccggt
       61 gggggaaaga gggaatgtaa tcgttttacg cttccccaga aagccatggg ttggtagtcg
      121 gaatagggat aatacccgag atgaaggcga atgtccgagt tggcagctcg aacatgctgc
      181 tctacaagac gcctgataga gatgggagct ccgctgcagc agttcacaat gccactgaag
      241 ttcggcgcgg tggcgatgcg gctcagatac tgcgccacga tctcgacggg caggtagtcc
      301 cgaagttgtt cgccccctga catacgaaat tcggattcac gcttctggat ggctgaccgc
      361 aactgggcaa gaatcgaacc agctgcttgt ccctcgccat acagataaaa gaggcgcacc
      421 cacttcaggg agaatgggca gtgagatctc aaaacctcaa gaaatcggcg taatgcgtct
      481 tttgccatgg cgtaggcagt gacgggcaga gcgggactat cttcagagag ttcgccctcc
      541 tgtttcccat actcgaaaca ggtgcctgta acggtaatat ccgatacgcc atcttgtaca
      601 aaactcttga tgaaggcata cgccgccgga agattctttt caatatgaaa caattcacga
      661 tagttcggta gtccaggcca ggcgagatga accagccgtg ttggacggtg cagccgttcg
      721 aaccagtcat cacgaggctc attgatatca caggaaacat actgtacgcg cttgagccaa
      781 gggaaggcaa gttgctcggc tggttcagtc ccgcatgcga tgacttcctg gccggcatcc
      841 agcaattgag gtacaagatg gcgaccgatg aagccagtgg ctccagtgac cagtgtgcgt
      901 gtcatcggtt gaagtatccc agcgcctgat ctttgggaga aaccagtagg atgggatccg
      961 gccattggat tccaatctgc gggtcgtcaa acctgattcc accctctgat tccggagaat
     1021 aaaactcgga cacttgataa aacacttcgg tgttgtcctc tcgtgtcaga tatccgtgcg
     1081 cgaatccctc aggaacatac atcatgcggt gattgtccgc ggacaactcg gcgccaaacc
     1141 acttgccgca ggttggagaa gatggacgaa tgtcgacgat ggcatcaaag atacgcccgc
     1201 gagtacatcg cacgagcttc acttccgcat gtggggctcg ctgaaaatgc atgccccgca
     1261 aggttccgct cttgtgattg aacgagagat tgctttgcga taagcggcaa ttgaggccat
     1321 gcttctcgaa ttcacgtgcg caccagctgc gtgcaaagaa accacgttca tcagagatcg
     1381 catcaatctc gatcaggtaa gcatcttgga gtggagttga gatgaacttc atggagtgac
     1441 tttcaatttc agatcatcag gagcctgcag attgctcaag caaagcttca tattgtctga
     1501 gctgcttctg ggtcacagct gcaaggtcag cgtgatcgag ataggaacga taccactgaa
     1561 cggtcagttc cagacactgt tcaagcgtca gctgagggtg ccagtgaagc agggatttcg
     1621 ccttggaaga atcgagtttg agcgtatggg tttcatgata tgcgggttca ggttgtcgaa
     1681 tgatcgtgag gtcgcttccc cagtaattcc cgaggagacg cagaacgtgc tcgacactct
     1741 gaatcccggt atcatcgggg ccgaaattcc atggtcccga aaaattcttc ccctgggttg
     1801 ccaggttctt cgcgagaagg agataaccgt acaggggttc gagcacaaac tgccaggggc
     1861 gctgtgcctg aggccggcga atcgtgagag gatggcctgc ctcgatagat cgaatgacgt
     1921 ccgggatcaa acgcccctct gcccaatcgc cgccgccgat gacattgcct gcgcgtgcgg
     1981 ttgccacgaa cggaggcgag tcgggggcag caaaatagga ttgctgatag cacgaagtga
     2041 cgagttcagc gcaggctttg ctggaggaat acgggtcact gcctcccaaa cggtcgattt
     2101 cccgatagcc gtaagtccat tcctgattct cgtagcattt atcggttgtg actacgacca
     2161 tggcttgaac tgaccgcgtc tgacgcacag cgtcgcaaac gacagcggtg ccaagaacgt
     2221 tcgtggaaaa ggttgccagc ggctcgcgat atgatgtccc aaccagtgat tgagccgcca
     2281 ggtgaaaaac aatttcagga gactcgttct ggaataatcg ctgtacctcg tcagcgtttc
     2341 ttatatcaat gcgatgcgaa ttcaggcttg tgtggagttg tagcaagttg tacaaacatg
     2401 gagaggtcgg aggatcgaga gccaggccaa cgacatgagc cccgagtttt tgcagccaca
     2461 cggagagcca gctgcctttg aatcccgtgt gactgcaaaa actcggggct catgtcgtta
     2521 ccagacc
//
LOCUS       DAORHD010000730         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49635_length_2524_cov_4.380316, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000730 DAORHD010000000
VERSION     DAORHD010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49635_length_2524_cov_4.380316"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="PKO06_14675"
     CDS             <1..1807
                     /locus_tag="PKO06_14675"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNV70943.1"
                     /translation="LIGLLVGGGLLASSAAAERWGQDPLFIRGLFAAGWASLYFTVYA
                     AHGLSATRVIDSPVVAGVLLMITAIGMVAHSLHFASEATTGLAFLIAFVGMVIGPEGA
                     FTNFASLLLVAGLLLLSYHHAWSRLALVGTFLGYGLHFFHPVTGLGEGLTAQATRTLP
                     VANNLLLCLYWWGFELMAIVTAHRHSVSGQLRGAQFMSNALGFFLVSYLLRPDPPQGG
                     EYVLWAEMATFYLISAYLRYSKFPAKEDPDDAADNSWFGNSESALCASVVAAVLCVWY
                     LCPATLVVIGWGIISLLVIELGLRLPFPTLKNLGHGVAMLSFGRVFLSNLTIDGHTAG
                     VSHRLLTVLPMIALYIQIFLRHAEDGVARDSGEGQRVITRAYLYFPVILLAALARFEL
                     GRGLATPAWALLALSLQVLGRQFHLIDLQRQSVVLGLLTALHGWGTDLRSLEASGWLA
                     SSSLIGTVSIVLLFACEAATPREPPWREQEFEGAPTGIGNLLENSGRLLFSLSASFLL
                     AFLLYLEITGSLLSMAWALQGGLLLVLGFALREKPARQSGLLLLFLCLAKLFFYDLQN
                     LDVPFRILSFVVLGLLLIGISWFYTRYQDKIKEYL"
     gene            1829..>2524
                     /locus_tag="PKO06_14680"
     CDS             1829..>2524
                     /locus_tag="PKO06_14680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV70944.1"
                     /translation="MKNYGMLLVLLLLLGAAGLVMLGQREDRSDLTAVMELVGDAQQD
                     ALKVGHELLKMSDDEENRLGREVVGRFGFGLSEAPRLASYVTAVARPLLAHVRRPRIE
                     YVFRVLDSGEVNAFALPGGQIFVFRGLIECVESEAELAAVLGHEIAHVDLRHCVELFL
                     VRARLEKIVGGGFPRTIVDLIVRLPANLTIFTHQLIKAGYRKFQEFDADAEGMNLIAR
                     AGYLPTGSVMAMQR"
BASE COUNT          378 a          713 c          798 g          635 t
ORIGIN      
        1 gttgatcggt ctgctggtcg gaggtgggct gctggcgtca agcgccgcgg ccgagcgctg
       61 gggtcaggat ccgctgttca ttcgcgggct ttttgcggcg ggctgggcga gtctgtattt
      121 caccgtctat gccgcgcatg ggttgtcggc aacccgcgtc atcgacagcc cggtcgttgc
      181 cggggtcttg ctgatgatca ccgcgatcgg catggtggcg cattccctgc acttcgcctc
      241 ggaagcaacg acgggcctgg ctttcctgat cgccttcgtc ggcatggtca tcgggcccga
      301 aggagcgttc accaacttcg cttcattact gctggtggcg ggcttgttgc tgctctcgta
      361 tcaccatgcc tggagccgac tggctctggt cggtacgttt ctgggctatg gcctgcactt
      421 ctttcacccg gtaaccggcc tcggcgaagg gttaacggct caggccactc ggactcttcc
      481 ggttgccaac aatctgctgc tctgtctcta ctggtggggt ttcgagttaa tggcgatcgt
      541 gaccgcccat cgacactctg tttcggggca actgcgcggc gcgcagttta tgagcaacgc
      601 gctgggcttt ttcctggtgt cctatctgct ccgacctgat ccgcctcagg ggggcgagta
      661 tgtgctctgg gctgaaatgg cgacgttcta tctgatcagc gcctatctgc gctattccaa
      721 gtttcccgcc aaggaagacc cggacgatgc cgcggacaat tcatggtttg gcaactccga
      781 atcagcgctg tgcgcttcgg tcgtggccgc ggtgctgtgt gtctggtatc tctgtccggc
      841 gaccctcgtc gtcatcggct ggggaatcat cagtctgctg gtgatcgagc tcggtctgcg
      901 cctgccgttc ccaaccctga agaatctcgg gcatggtgtg gcgatgctct cgttcggtcg
      961 cgtctttctg tcgaatctga cgatcgatgg tcacacggct ggggtttctc atcggctgtt
     1021 gacggtcctt cccatgatcg cgctctacat acagattttt ctgcgacatg ccgaggacgg
     1081 agttgctcgt gacagtggag agggacagcg cgtgataact cgcgcctact tgtactttcc
     1141 agttattctg ctggcggctt tggcgcgatt cgaactcggg cgcggcctgg ccactccggc
     1201 ctgggctctt ctggccctgt cacttcaggt gctcggtcgc cagtttcact tgattgatct
     1261 gcagcgacag agtgtcgtgc tcgggctcct gacggcactg cacgggtggg gaaccgatct
     1321 gcgcagtctc gaagcgagcg gttggctggc gagcagttcg ctgatcggga cggtttccat
     1381 cgtcttgctc tttgcctgcg aagcggctac tccacgtgag cccccgtggc gtgagcagga
     1441 gttcgaggga gcgccaacgg ggatcggcaa ccttctggaa aacagtggtc gattgctgtt
     1501 ttctctttcc gcttcctttc tcctggcttt tctgctgtat ctcgagatca ccgggagcct
     1561 gctgtccatg gcgtgggcgc tccagggcgg gttgctgctc gtgctcgggt tcgctctgcg
     1621 cgagaagccg gctcggcagt ctggcctgct gctgctgttt ctgtgtctgg caaagctgtt
     1681 tttctacgat ctgcagaatc tcgacgtccc attccgcatt ctgtcgtttg tggtgctcgg
     1741 gctcttgttg attggcattt cctggttcta tacgcgatac caggataaga tcaaggaata
     1801 tctgtaacat cgtttacgag gttcaatcgt gaaaaactac ggaatgctgc tggtactctt
     1861 gctgcttctt ggcgcagccg gcctggtgat gctggggcag cgagaggacc gttcggatct
     1921 caccgccgtc atggaactgg tgggcgacgc acagcaggat gccttgaaag tcgggcatga
     1981 actgctgaag atgtcggacg acgaagagaa ccggctcggt cgggaagtcg tcggccgctt
     2041 tggtttcggg ttgtctgaag ctccccggct ggcttcctat gtgacggccg tggctcgccc
     2101 gctactggct catgtgcggc gacctcgtat cgaatatgtc tttcgcgtgc tcgacagcgg
     2161 cgaagtcaat gcattcgcgt tgccgggcgg tcagatcttc gtgtttcgcg gtctgatcga
     2221 atgtgtcgaa agtgaggccg aactcgcggc cgtgctcggg cacgagatcg cccacgtcga
     2281 tttgcgtcac tgtgtcgaac tgtttctcgt gcgggcccga ctcgaaaaga tcgtgggcgg
     2341 tgggttcccg cgtacgatcg tcgatctgat cgtgcgtctg ccggccaacc tgaccatctt
     2401 cactcatcag ctgatcaagg ctggctaccg gaagtttcag gagttcgacg cggacgcgga
     2461 aggcatgaac ctgatcgcca gggccgggta cctgccgacg ggatcggtga tggcgatgca
     2521 gcgc
//
LOCUS       DAORHD010000731         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49763_length_2520_cov_3.253144, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000731 DAORHD010000000
VERSION     DAORHD010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49763_length_2520_cov_3.253144"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2520
                     /locus_tag="PKO06_14685"
     CDS             <1..>2520
                     /locus_tag="PKO06_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70945.1"
                     /translation="YIGSGKTFFVDTIGPEARIRIPSINTMRIDAASNTLVVAPVTGG
                     RVEVSVETLGELYQPPELAWSFEGSEEWNAIPLAGFGDRWSGTMMIPQGIASAAARFR
                     YRGVDRIGNLSTTIERRTYERKLDEDTDPPRTLESYATTGGVFSIDSVAPQPPINVRL
                     DQKKLGIAVVKWDEGPGDPKSYNLYRALTPITSRKGLTPVKTGIFAPIIVDDPPVDGN
                     FFYAVTQLDMAGNESEVSESRSVFIDSIKPELKIRAVPSGEDFIILADEDEGQTLSVE
                     LRFPGGKTMQVELGGSSGELEEIAIPGENRKRRGRVLPQILRQFNGTVEIVVHSPDPA
                     GNKVEQITSIETKQIPVETGGDIQSADQKVTLEVPAGMVPVIPKGPNDFQRVGGYQNL
                     FFIRHENIPTKPVESTASAPADPREPDPLPPSLEVIGTPYRVELNVNTEKPMELRASA
                     SQADFSQLKELTAKLRMKIPDLGSDAVADQNYLASRLKVVKWVPATRDEAAGITKTNK
                     RGRWEIVPDIEISTGTREIIAPARDITTYVIVSERTPPSIVDLEPAPDSTVKTFRPTI
                     KARLIDKGTGIAQGAENRVVLHIDGMPADPARLTLSTEDPTEVTLSYQPSEDLTPGAH
                     LITIKAQDVVENQAVRRWRFVIDNEPPRISSILPAENVVVGLSRPVISVVTEDEGGGI
                     DPERTSIAVDGATLTPKFDPVAGRVVAFPSQPLSSGQHNVQVQFEDRGGKKVAKTWSF
                     ATDVDAPVIKLVQPASSTPLPNGRQPLIIEVSDTGAGVDASSLLLDLDSQLIGPAKLF
                     DVSDNFTYDPVKRQILFAPTAGFKTGEHSLLLSAGDRLGRF"
BASE COUNT          551 a          796 c          704 g          469 t
ORIGIN      
        1 cctatatcgg ttcaggaaaa accttcttcg tcgatacgat cgggccggaa gctcgcatcc
       61 gcattccgag catcaatacg atgcgcatcg atgccgcgtc gaacacgctg gtcgttgctc
      121 ccgtcaccgg cggtcgcgtt gaagtctcgg tcgagacact tggagaattg taccagccgc
      181 cagaactggc ctggtccttt gaaggctctg aagaatggaa cgcgattcca ctcgccggct
      241 tcggtgaccg ttggagcggc acgatgatga ttccccaggg catcgccagt gccgcggcac
      301 ggttccgtta tcgtggcgtc gaccggatcg gcaacctctc gacgaccatt gaacgacgca
      361 cgtatgaacg caagcttgat gaagacacgg acccgccgcg cacgctcgag tcgtatgcga
      421 ccaccggcgg cgtgttttcc atcgactccg tggcacccca gcctccgatc aacgttcgac
      481 tcgatcagaa gaagctcggc atcgccgtcg tcaagtggga tgaaggcccg ggcgacccga
      541 agagttacaa cctgtaccgc gctctgaccc cgatcacaag ccgcaaaggc ttgaccccgg
      601 tgaaaaccgg gatctttgcc ccgattatcg tcgacgatcc tccggtcgac ggcaattttt
      661 tctatgccgt cacgcagctg gatatggccg gcaacgagag tgaagtgtcg gagagccgct
      721 ccgtcttcat cgacagtatc aagccggaac tcaagatacg cgctgtcccc agcggcgaag
      781 actttatcat tcttgccgat gaggatgaag gccagaccct ctctgtcgaa ttgcgcttcc
      841 cgggcggcaa gaccatgcag gttgaactcg gcggcagctc gggagaactc gaggaaatcg
      901 ccattccggg cgagaaccgc aaacgtcgag ggcgcgtcct gccacagatt ctgcgtcagt
      961 tcaacggcac cgtcgaaatt gtggtgcact ctcccgaccc tgccggaaac aaggtcgaac
     1021 agatcacgtc gatcgaaacc aaacagattc cagttgagac cggtggcgac attcagtccg
     1081 cagatcagaa ggtcaccctg gaagtaccgg ccgggatggt accagttatt cccaaaggac
     1141 cgaacgattt tcagcgggtt ggcggctatc agaatctgtt tttcattcgt cacgaaaaca
     1201 ttccgaccaa gcccgtcgag tcgaccgcgt ccgctcctgc cgaccccagg gaacccgatc
     1261 cgttgccacc gagcctggag gtcatcggca caccgtatcg cgtagaactg aacgtgaaca
     1321 ccgagaagcc gatggaactg cgtgcttcgg catcccaggc agacttcagc cagctcaagg
     1381 aactgaccgc caagctgcgc atgaagattc cggacctggg ctccgatgca gtcgctgacc
     1441 agaactacct cgccagtcgc ctcaaagtgg tgaagtgggt tccggccacg cgtgacgaag
     1501 ccgcgggcat cacgaaaacc aacaagcgcg gtcgctggga gatcgttccc gatatcgaga
     1561 tcagtacagg aacccgtgag atcatcgccc cggcgcgcga catcaccacc tacgtgattg
     1621 tctccgagcg cacgcctcct tccatcgtcg atctcgagcc ggcaccggac agcaccgtca
     1681 agacgttccg cccgacgatc aaagcccgcc tgatcgacaa gggaaccgga atcgcccagg
     1741 gagcggaaaa ccgtgtcgtg ctgcacatcg acggcatgcc ggccgatccg gctcgcctca
     1801 cgctctcaac ggaagaccca acggaggtca cactgtccta tcagccttca gaggacctca
     1861 cccctggagc acatctgatc acgatcaagg cccaggacgt cgtggaaaac caggccgtca
     1921 ggcgatggcg ctttgtcatc gataatgaac ctccgcgaat ctcatcgatt ctcccggccg
     1981 aaaacgtcgt ggttgggtta tcgcgacctg tcatctctgt cgtcacagag gacgaaggcg
     2041 gcggcattga tccggaacgg acctcgatcg ctgttgacgg ggcgacgctg acgccgaaat
     2101 tcgatccggt ggcggggcgc gtggtcgcct tcccgtccca gccgctttct tctgggcagc
     2161 acaacgttca ggttcagttc gaagatcgcg gcggcaagaa agtggcaaag acctggagct
     2221 ttgccaccga cgtagatgcc cccgttatca agctggtaca accagcctcc tcgactccac
     2281 tccccaatgg tcggcagccg ctgatcatcg aagtctccga taccggcgcc ggagttgacg
     2341 cctcgtctct attgctcgat ctggatagtc agctgatcgg accggcaaaa ctctttgatg
     2401 tctcggacaa cttcacctac gacccggtca aacgccagat tctgttcgca ccgaccgcgg
     2461 gttttaagac cggagaacac agtctcctgt tgagcgcggg cgaccgcctc gggcgcttca
//
LOCUS       DAORHD010000732         2518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49832_length_2518_cov_3.634998, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000732 DAORHD010000000
VERSION     DAORHD010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49832_length_2518_cov_3.634998"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKO06_14690"
     CDS             complement(<1..277)
                     /locus_tag="PKO06_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70946.1"
                     /translation="MKATTHAKATSAQKRTSAGQGKAAAAGQNKASVNRGKTTVVRHA
                     KPVSSGKGKTQISPRGKAMADKHRKTGLATHIKMTGKPAAVGQAKTMG"
     gene            complement(297..2084)
                     /gene="dnaG"
                     /locus_tag="PKO06_14695"
     CDS             complement(297..2084)
                     /gene="dnaG"
                     /locus_tag="PKO06_14695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNV70947.1"
                     /translation="MNAFGPARRGWITDQLLADIRDRVNIVQIVGEYVRLLPSGKNFK
                     GLCPFHQEKTPSFYVVPAKQIFHCFGCQKGGDVMKFVMEAERLHFPEAVRMLANRAGI
                     AIETEKDPEADARRELYDILEEAAHFFTANLQDSDIGEEVRDYLHDRGIKPETIRRYR
                     MGYARDSWDTLANALGRSGKRVELLQRAGLLKPRLEGSGHYDTFRSRLIIPILDQHQR
                     VVGFGGRVMRKGEEPKYLNSPETEVFNKRRMLFNLPFAMNEIRRENAVIVVEGYLDAI
                     SLAQNGVTNVVATLGTAITEDHVAMLARNCERVFFSYDADEAGQKATLRAISLQRQTP
                     LVARVITFPDAKDDPDSFIRREGADAFWQRLAEAPDVYEFLLQTRTHGLPRPLDIPTK
                     ERLVAEFRELIPLVQSPVARAELIKKLGRLLDLEPRTLEAALGRGRAISSTQEQQRVR
                     PPAKGQTRYQEWIIKQLLESPEHLDQVRSLVQPEDFSDPALRKLYEYICHYATTAGNQ
                     LRPGEIMGAIEDPSLQTALSELIVTLEDQPPQPLLGCAEALSKSRISDRLRDIEHQIK
                     IAEQAGDQNRVNELSREQFGLLKRKMTGR"
BASE COUNT          500 a          728 c          709 g          581 t
ORIGIN      
        1 cgcccatcgt cttggcctgg ccgaccgccg ccggcttgcc cgtcatcttg atatgagtcg
       61 ccagaccggt cttgcgatgt ttgtcggcca tggctttccc gcgcggtgat atctgggttt
      121 ttcccttgcc ggacgaaacc ggtttcgcgt ggcggacgac ggtcgtcttg ccacggttca
      181 cggacgcttt gttctgaccc gcggcggccg ccttgccctg ccccgcggag gtgcgtttct
      241 gtgctgatgt tgctttcgcg tgagtggttg ccttcatgga acttcctcgt ttagaactac
      301 cttcctgtca tcttccgctt caagagaccg aactgttcgc gggaaagctc gttgacccgg
      361 ttctgatcgc cagcctgctc ggcaatttta atctggtgtt cgatgtcacg aagccgatcg
      421 ctgatacgcg actttgagag ggcttcggcg caacccagca atggttgagg gggctgatct
      481 tcgagggtaa cgatcaattc gctcagagcg gtctgaaggg aaggatcctc gatggcaccc
      541 atgatttcgc ccgggcgcag ctgattgcct gctgtagtag catagtggca aatgtattca
      601 tacagcttac gcaaggccgg gtcagaaaaa tcttcgggtt gtaccaagga ccgtacctgg
      661 tccaaatgct cgggactctc taacagttgc ttaatgatcc attcctgata tcttgtctgc
      721 ccctttgcgg ggggccgaac tctctgctgt tcctgagtac tgctgatcgc ccgaccgcga
      781 ccaagagcgg cttcgagtgt gcgcggctcg agatcgagca gacgcccgag ctttttaatc
      841 agctcggctc gggccaccgg actttgtacc aagggaatca attcacgaaa ctccgcgacc
      901 aatcgctctt tggtcggaat atcgagcggg cgcggcaagc catgggtacg ggtctgcagg
      961 agaaactcgt agacatccgg ggcttcggcc agacgctgcc agaaggcatc ggccccctcg
     1021 cggcgaatga aactgtcggg atcgtctttc gcgtcgggga aagtgatcac ccgcgccacc
     1081 agaggcgtct ggcgctgcag agaaatagcc cgcaacgtgg ctttctgccc tgcttcgtct
     1141 gcatcgtagg aaaagaagac ccgttcacag ttgcgcgcca gcatggcgac gtgatcttcg
     1201 gtgatcgccg tgcccagcgt tgccacgacg ttcgtgacgc cgttctgcgc cagactgatg
     1261 gcatcgagat agccctcgac cacgatcacc gcgttttcac gccgaatctc gttcatcgca
     1321 aacggcaagt tgaacagcat gcggcgcttg ttgaacactt cggtttctgg gctgttgaga
     1381 tacttcggct cttcgccctt ccgcatgacc cgaccgccaa aaccaaccac ccgctgatgc
     1441 tgatcgagaa tcggaatgat cagacggctg cgaaacgtgt cgtagtggcc gctgccttcg
     1501 aggcggggct tcaacagacc cgcgcgctgc agcagttcaa cgcgtttgcc tgaacgcccc
     1561 agcgcgttgg ccagggtatc ccaggaatcg cgcgcatagc ccatgcgata gcgacgaatg
     1621 gtttcaggct tgatgccgcg gtcgtgcaga tagtcacgca cttcttcgcc aatgtccgaa
     1681 tcctgtaggt tggccgtaaa aaaatgggcc gcttcttcga ggatgtcata cagctcgcgg
     1741 cgcgcatcag cttccgggtc tttttcggtc tcaatggcga ttccggcccg gtttgccagc
     1801 atacgaacgg cttcgggaaa atgcaggcgc tcggcttcca tgacgaactt catcacgtca
     1861 ccgcccttct ggcagccgaa acaatgaaag atctgcttgg cgggaaccac atagaaactg
     1921 ggcgtctttt cctgatgaaa ggggcataac cccttgaaat tcttaccgga agggagcagg
     1981 cgtacgtatt cccccacgat ctgtacaata ttgacccgat cgcgaatatc ggcgagcaac
     2041 tggtcggtga tccaacctcg ccgtgccggc ccgaaagcgt tcatgaacag caagcgttca
     2101 tcctgaaaag gaccagaatg cggacatgag acattgatgc taacaaactg acttgcacct
     2161 gtcaacgaat ttcccccttt tcggatttat tgcaagcaga acttttgaaa tttcctttct
     2221 ttgtagttct cccgtacagg cgcgacacgg ttccagctcg tatgtgcccg cagcgaactg
     2281 ctccattcgt tcccgcgacg ttttgcctca gggtcatttg attctgttcc gttttcctct
     2341 tggcctgtgc gaagctggtg agccattcgg caagaggcgg ttgagtccga aaccaggctg
     2401 ccggatcgca ggaggcgatt cggccccgta tcgtcttcgg ataaagggca tgctccctgc
     2461 cgcgacggtg aatcgtacag cgatgccaag gggaaaactc gtatgcggag aatacagt
//
LOCUS       DAORHD010000733         2516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_49897_length_2516_cov_4.000813, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000733 DAORHD010000000
VERSION     DAORHD010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49897_length_2516_cov_4.000813"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PKO06_14700"
     CDS             complement(<1..209)
                     /locus_tag="PKO06_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HNV70948.1"
                     /translation="MSNTPIGIPLPTNEPTLRYAPGSTERRHLKQELERQVAQPLEIP
                     LFIDGKTVHTGATVEVRAPHDHRQVL"
     gene            complement(230..1756)
                     /locus_tag="PKO06_14705"
     CDS             complement(230..1756)
                     /locus_tag="PKO06_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNV70949.1"
                     /translation="MNDILTHFGLTGTLPGGFHGSWLGCGKPLKVTSPVNDQTLATVE
                     TVSRESYETIVAAAARAFIEWRQLPAPRRGEIVRQLGEALRRDKEMLGKLVSLEMGKP
                     VQEGLGEVQEMIDICDYAVGLSRMFAGQVFPSERAQHRIMEQWHPLGVIGVISAFNFP
                     VAVWSWNAALALVCGNTVLWKPSSKTPLAAIAVTKLAAQVLIANGVHPAVCSLVVGRG
                     AEIGDAINTDTRVPLVSYTGSVSGGRHVGTLVQQRFGRLILELGGNNAVIVSRHADLD
                     LALKAVYFGAVGTAGQRCTSTRRVIVHESVYDSFVSRLSELYANAVVDSPFTPGIHHG
                     PLVDKAALETMMKAIDTIKQQGGRVRVGGEPIPRLGACYVTPCLVEAPSTLPMIQEET
                     FAPILYCLKYQSFEEAIALQNGVPQGLSSAIITRDQREAELFVSATGSDCGLANVNTG
                     TSGAEIGGAFGGEKETGGGRESGSDSWKVYMRRQTTVVNFSGDLTLAQGLRFELPKAK
                     "
     gene            1880..>2516
                     /locus_tag="PKO06_14710"
     CDS             1880..>2516
                     /locus_tag="PKO06_14710"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV70950.1"
                     /translation="MADYTLPDLQPLQTFYVAATSRTFTEAAERLCLSQSAVSHAVAR
                     LEQSLRLSLFERIGTKSRLTDAGKRLLSSCERVFDELRQCRDELTRRDPHAISGQLRL
                     GTTVEFGNGVLAAKIAPFLQQYPQLDLSLTFSHDLVSPLLAGNLDLIIDCWVHPREDL
                     WQVKLFREKYVLVAAPAFAQTMRLRRLSDLGRVSWLTLDPAGAWWHRLLVQL"
BASE COUNT          458 a          768 c          785 g          505 t
ORIGIN      
        1 agaacctgtc gatgatcgtg tggtgcccgc acttcaaccg tggcccccgt gtgcacggtt
       61 tttccgtcga tgaacagggg aatctccagc ggctgagcga cctggcgttc gagttcctgt
      121 ttcaggtgcc gtcgctcggt tgacccgggt gcgtagcgaa gagtcggctc attcgtcggc
      181 aatggtattc cgatcggggt gttgctcatg gttgttccag cctgagggcc tatttcgcct
      241 tcggcagttc gaagcggagt ccctgagcga gagtcagatc gccgctgaag ttcaccaccg
      301 tggtctggcg gcgcatgtag accttccagg agtcgctgcc gctctcgcga ccgccacccg
      361 tctctttttc gccgccgaac gcgccaccga tttcggcgcc gctggtaccg gtgttgacgt
      421 tcgccagacc gcagtcactg ccggttgccg acacgaacaa ctcggcttct cgttgatcgc
      481 gggtgatgat ggcgctgctt aatccctgtg gaacaccgtt ttgcaaggca atggcctctt
      541 cgaacgactg gtacttcagg cagtagagaa tgggggcgaa ggtttcttcc tgaatcatcg
      601 gcagggtgga tggggcctcg accagacagg gggtcacgta gcaggcgccc aaacggggaa
      661 tcggctcgcc gccgacgcgg acccggccgc cctgctgttt gatggtatcg atcgccttca
      721 tcatggtctc gagtgccgcc ttgtcgacca gcggaccgtg atggataccg ggggtgaacg
      781 ggctatcgac gacggcgttt gcatacagct cgcttaagcg cgagacaaac gaatcataga
      841 ccgattcgtg cacgatgacg cgacgggtcg acgtgcagcg ctggccggca gtgccgacgg
      901 cgccgaagta gacggccttg agggccagat cgagatcggc gtgccggctg acaatcaccg
      961 cgttgttccc gccgagctcg agaatcagac ggccgaaccg ctgctgaacg agagtgccga
     1021 catgccgccc gccgctgacc gaaccggtat acgacacgag cggaacgcgt gtgtcggtgt
     1081 tgatggcatc accgatctcg gcgccccgcc cgactacgag cgaacagacc gccggatgga
     1141 cgccgttggc gatcagcacc tgcgcggcca gcttcgtcac ggcgatggcc gcgagggggg
     1201 tcttggatga cggcttccag agcacggtgt tgccgcagac cagcgccagc gcagcgttcc
     1261 agctccagac cgccactggg aagttgaagg cggagatgac cccgatcacg ccgagcgggt
     1321 gccactgttc catgatgcga tgctgggccc gttcgctcgg aaacacctgg ccggcgaaca
     1381 tacgcgacaa cccgactgca tagtcgcaga tatcgatcat ttcctgcact tcgcccaggc
     1441 cttcctggac gggcttaccc atctcgagcg agaccagttt cccgagcatc tccttgtcgc
     1501 ggcgcagagc ttctcccagc tggcggacga tctcgccgcg gcgcggagcg gggagttgcc
     1561 gccactcgat gaaagccctg gcggcggccg cgacgatggt ttcgtaagac tcgcggctga
     1621 cggtttccac cgtggccagc gtctggtcgt tgaccggaga ggtgaccttg agcggcttgc
     1681 cgcaaccgag ccacgatccg tgaaagccgc ccggcagcgt gccggtcagg ccgaagtgcg
     1741 tcagaatatc gttcatgcga gaacctccgt gatgggtgtg gggttcagca tgcacgattc
     1801 tttatcatga ctacaaatga aaggaattga tatatactat gactgaaatt catgataagg
     1861 catcgccggg cgacatctca tggctgatta cacccttccc gatctgcaac cgctgcagac
     1921 gttctacgtc gcggccacgt cgcgcacctt caccgaagcc gcggagcgac tctgtctcag
     1981 ccagtccgcg gtgagtcatg ccgtggcacg gctcgagcag tctttgcgcc tctctctctt
     2041 tgaacgcatc ggaacaaagt ccaggctgac ggatgcggga aaacgtctgc tgtcgtcgtg
     2101 tgaacgagtg ttcgatgaac ttcggcagtg ccgcgacgaa ctgacgagac gtgatccgca
     2161 tgcgatcagc ggccagttgc ggctcggcac gaccgtcgaa ttcggcaacg gcgtcctggc
     2221 cgccaagatc gccccttttc tgcagcaata cccgcaactc gatctttccc tcaccttcag
     2281 tcacgatctg gtgagtccgc tgttggcggg caacctcgat ctgatcattg actgctgggt
     2341 gcatccgcgc gaagatctct ggcaggtgaa gctgtttcgt gaaaagtatg tccttgttgc
     2401 cgcaccggca ttcgctcaga cgatgaggct gagacgcctc tccgatctcg gtcgcgtctc
     2461 ctggttgaca ctggatccgg cgggggcctg gtggcaccgc ctgctggtgc aactgc
//
LOCUS       DAORHD010000734         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50014_length_2512_cov_3.656085, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000734 DAORHD010000000
VERSION     DAORHD010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50014_length_2512_cov_3.656085"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..725)
                     /gene="pncA"
                     /locus_tag="PKO06_14715"
     CDS             complement(105..725)
                     /gene="pncA"
                     /locus_tag="PKO06_14715"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HNV70951.1"
                     /translation="MEALILVDLQNDFMPGGALAVREGDLVVPVANRLMPHFKLVIAT
                     QDWHPQDHRSFAASHPGKQPGDVIDLHGLSQVLWPVHCVAGTPGAALYRTLDQKRITE
                     TIRKGMNREIDSYSGFFDNGHRQATGLEQRLARDGVKAVTILGLATDYCVKFTALDAV
                     RLGFKTRLVQDGCRGVELKPGDVEHALDEMRQAGVSLCLADDLLRP"
     gene            complement(731..2218)
                     /locus_tag="PKO06_14720"
     CDS             complement(731..2218)
                     /locus_tag="PKO06_14720"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNV70952.1"
                     /translation="MASLSGMYKTSLALLTDLYQLTMAYGYWKTGSADKEAVFNLTFR
                     KNPFQGGYTLLAGLEYVLEFLRGFRFSEEDVSFLAGLQGNDGRPLFESPFLDDLRRMR
                     FSCDVHAIPEGTVAFPQEPVIRVQGPIMQCQILETPLLNILNFQSLIATKAARICMAA
                     QGESVLEFGLRRAQGIDGALAASRAAYIGGCSSTSNVLAGRLFGIPVRGTHAHSWVMS
                     FDSEEESFRQYAEALPNNCVFLVDTYNTLDGVRHAISAGTWLRSQGHEMIGIRLDSGD
                     LAYLSREARRLLDEAGFPKASIFASNDLDEHIITSLKLQGATIAVWGVGTKLVTGFDQ
                     PALGGVYKLVAVRKPGERWEYKVKLSEQAIKTSTPGILQVRRFQADSECIGDLIYDES
                     LGIGREIVLVDPMDSTRRKRINTGTPHEDLLVPVVKSGAVIYQSPSIQDIRARSQQQL
                     SRFHAGITRFVNPHTYPVGLEPSLHELKTQLILKARGFGHETAKG"
BASE COUNT          523 a          726 c          761 g          502 t
ORIGIN      
        1 tgcgtgacga actcgatcaa ttgctcgaac gggcgcgcga gatgaccccc agcgcggtcg
       61 tggtgtccca gaacgaaaag cccgcaagcg cgaatccgtc tgattcaggg gcgcagcaga
      121 tcatccgcta gacagagcga caccccggcc tgacgcatct catcgagggc gtgctcgaca
      181 tcgccgggtt tcagttcgac accgcgacag ccgtcttgca ccagccgggt cttgaagccg
      241 aggcgaaccg cgtcgagcgc ggtgaatttc acgcagtagt cggtggccag accgagtatg
      301 gtcacggctt tcaccccatc tcgcgcaagt cgttgttcga gaccggtggc ctgacggtgc
      361 ccgttgtcga agaacccgct gtaggaatca atctcgcgat tcatcccctt gcgaatggtc
      421 tcggtgatgc gcttctggtc aagagtgcga tacagcgcgg cacccggtgt gccggcgacg
      481 cagtggaccg gccacagcac ctgagaaagg ccgtgcagat cgatgacgtc gcccggttgt
      541 tttcccgggt gagaagcggc gaaactgcga tgatcctggg ggtgccaatc ctgggtggca
      601 ataaccagct tgaaatgagg catgagacga ttcgccaccg gtacaaccag atcaccctca
      661 cgaaccgcca gagcgccgcc aggcatgaaa tcgttttgca gatcgaccag aatgagtgct
      721 tccatgccgc tcatcctttc gccgtctcgt gaccgaagcc ccgggctttg aggatgagct
      781 gcgtcttcag ttcgtgcagg ctgggttcga gaccgaccgg atacgtgtga gggttcacga
      841 agcgggtgat gcccgcatga aacctggaca attgttgctg agaccgggct cgaatgtcct
      901 ggatggaggg cgactgatag atcacggcgc cggatttcac gacgggaacc agcagatctt
      961 catgcggggt tccggtgttg atccgcttgc ggcgcgtgct gtccatcggg tcgaccagaa
     1021 cgatctctcg accgatgccg agagattcat cgtagatcag atcaccgatg cactcggaat
     1081 ctgcctgaaa gcgtcgcacc tgtagaattc ccggcgtcga ggttttgatg gcctgttcgg
     1141 aaagtttcac cttgtattcc cagcgttcac caggctttcg cactgcaacc aacttgtaca
     1201 ctccgccgag cgccggctga tcgaacccgg tcacgagctt ggtgccgacg ccccacacgg
     1261 caatggtggc tccctgcagc ttgagactgg tgatgatgtg ctcatcgagg tcgttgctgg
     1321 caaagatcga ggccttcgga aatccggcct catcgagcag gcggcgtgct tcccggctga
     1381 gatacgccag gtcgccggaa tcgagccgga tgccgatcat ttcatgcccc tgcgaacgaa
     1441 gccaggtgcc ggcgctgatg gcatgtctga caccgtcaag ggtgttgtag gtgtcgacga
     1501 gaaagacgca gttgttgggc agggcttcgg catactggcg aaagctttct tcctcggaat
     1561 cgaaagacat gacccagctg tgcgcgtggg ttccccgtac cggaataccg aacagccgtc
     1621 cggcgagcac gttcgaggtg ctggaacacc caccaatgta cgcggcacgg ctggctgcga
     1681 gagcgccatc gatgccctga gcccgccgca gcccgaattc gagtaccgat tccccctgcg
     1741 cggccatgca gatgcgcgcg gccttcgtgg cgatcagact ctgaaaattc agaatgttca
     1801 acagcggggt ttcgagaatc tgacactgca tgatcgggcc ttgcacccgg atgacgggtt
     1861 cctggggaaa ggcaaccgtt ccttccggaa tcgcgtgcac gtcgcaactg aagcgcatgc
     1921 ggcgcagatc atcgaggaac ggtgattcga acagggggcg gccgtcgttg ccttgaagac
     1981 cggcgagaaa actgacgtct tcttcactga agcgaaaccc tcgcaggaac tcgagcacat
     2041 attcgagccc ggccagtagc gtgtagccac cctggaaggg attcttgcga aacgtcaggt
     2101 tgaagaccgc ctccttgtcc gccgatccgg ttttccagta gccgtaggcc atcgtcagct
     2161 gatacaggtc ggtcagcaga gccagcgagg ttttgtacat gccgctcaaa cttgccatga
     2221 tcctcctcca cgtcgacaca cattgggttg gtcacaggct atgccgttca gatgtcgaag
     2281 tcaaaccgcc gcctgcgaca tggaaattgc cgatgtagta ccaggtgttg tcagatgagc
     2341 gtacggctgt tacaatagaa acacgtaacg agtatgaagt aaggaatgat cgcatgacgc
     2401 ttcgctggat cgtcggtgcg ctgttgttgg ctctcaccac tgtttcgacg acgtgggccg
     2461 tcactccgcg ctttgccgaa gtacatcggg aaccgctcaa gatcgaagcg ga
//
LOCUS       DAORHD010000735         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50022_length_2512_cov_3.212047, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000735 DAORHD010000000
VERSION     DAORHD010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50022_length_2512_cov_3.212047"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2512
                     /locus_tag="PKO06_14725"
     CDS             <1..>2512
                     /locus_tag="PKO06_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70953.1"
                     /translation="SEPGIPEPTGSGEPSNSPVTPPAESGQKKPPRRILTIILAVVAV
                     LALGVAGAGYYAYVTYLQPEAIRTLIETKVSAGIGLPVRLGEVQLEFPAVRIKGLEVG
                     LATAAPNLHLTVGEAFLSPDFWELIAGKVIFDQVTLSSSAVTLHRQADGSLMLPTGSA
                     SSAASETSSAAAGPVAGEFPLRAFSATEVRLTVVNADGTRQQGILQKAELQHGKSGEG
                     MPFALSGAVEKLARIEMSGTLAWPTRLAMQVQALDLDMKSVKPWLPPTLVIPEQITEP
                     ALKVKLEYRFGAGLKIEDALLEAKPGVQIQAKADIASFSPLTGKVSVRCLPLKASFVL
                     DIAKPLLPPEYGLVIEEGQLSGGGDRFWKDGQTIESNLWLKPESLGFKARDLPFRADR
                     LQGIFKYTAGRLSWENLELQGGGVAVSSSKGSVVLSEGKGQADLKASAELGKVFAWAQ
                     KRLPKMYAGVGPSGQIEVSAQLDIQGADTRADGAVTLKNARFVAPPAQVPVEIEKVIV
                     QLQNVGRQGGKIVVQTLEAKGMGNRLQAKGSWDEIPRGPFLVEGSSEIDLAKLQAQLP
                     IENREFKEKARLAGQANVALKLSGTTEAPKLAGTVKLEKALFHLPERGLAFDAINGSV
                     RADMQSVAVEKLEATVAGAKMSLGGELKNFTKPQLQLRAALTGLDMSEVRTFLLRNIS
                     GFPADLDFSGGTDIEVQVSGSPESPKISGSAILAGARLSHPVLFRPIQQLVGPLAFDN
                     KTISTENLTFSWGSSTARIKGKVDDISKFALNFSYVVDPLDLTDIGTFFLASTGYKAS
                     GMGTGQGKIHGPIEKLVIDGTAIVPVGMFEAPLSKGSAS"
BASE COUNT          522 a          639 c          810 g          541 t
ORIGIN      
        1 ttccgaaccc ggcattcccg aacccacagg atcgggtgaa ccatcgaatt cccctgtcac
       61 accgccggcc gagagtggtc agaagaagcc gcccaggcgc attctcacga tcattctggc
      121 ggtcgtggcg gtgctggctc tcggcgtcgc aggtgcgggc tattatgcct atgtgacgta
      181 tctgcaaccc gaggcgatca gaactctcat cgagacgaag gtctctgcgg gaattggtct
      241 gccggtgcgt ttgggggagg tccaactcga gtttcccgcg gtgcgcatca aggggcttga
      301 agtgggtctg gcgactgcgg cgccgaatct gcacctgacg gtaggcgagg catttctctc
      361 acctgacttc tgggaactga ttgccgggaa ggtgattttc gatcaggtga cactctcttc
      421 gagtgccgtc acgttgcatc gacaggcgga cggaagtttg atgctgccca cgggaagtgc
      481 ttcttcagcg gcgtctgaga caagttcggc ggctgcaggc cctgttgctg gcgagtttcc
      541 gttgcgcgca ttttcggcga ctgaggttcg tctgacggtc gtgaacgcag acggcacacg
      601 acagcagggg attctgcaga aggcagagct gcagcacgga aaatcgggtg aagggatgcc
      661 ctttgccctg agtggagcgg tggaaaagct cgccagaatc gagatgtctg gcactctcgc
      721 ctggccgact cgtcttgcca tgcaggttca ggcccttgac ctggatatga aatccgtgaa
      781 gccctggttg ccgccgaccc tggtgattcc ggaacagatc acggaacccg ctctgaaagt
      841 gaagctggag tatcggttcg gtgcgggttt gaaaatcgag gatgccctgc tcgaagcgaa
      901 gcccggggtg cagattcagg cgaaggctga cattgcgtca ttttcccccc tgaccggcaa
      961 ggtcagtgtt cgctgcctgc cgttgaaagc ctcattcgtg ctggacatcg ccaaaccgct
     1021 gttgccacct gagtatggtc tggtcatcga agaaggacag ttgagcggtg gtggagaccg
     1081 tttctggaag gatgggcaga cgatcgaatc gaatctctgg ttgaagcctg agtcactggg
     1141 gttcaaggct cgcgatctgc cctttcgcgc tgaccgtctg cagggaattt tcaaatacac
     1201 cgcgggtcga ttgagttggg aaaatctcga actgcagggt ggcggggttg ccgtaagttc
     1261 atcgaaaggg tcagtcgtcc tatctgaagg aaagggacaa gccgatctga aagcctctgc
     1321 cgagttggga aaggtgttcg cctgggcgca gaagcgtttg ccgaaaatgt atgccggcgt
     1381 gggtccgagt ggtcagatcg aggtgagtgc gcagctcgac attcagggtg ccgacacgcg
     1441 agctgatggg gctgtcacgc tgaaaaacgc gcgttttgtc gcacctccgg cgcaggtgcc
     1501 ggtcgagatc gaaaaagtga ttgtccagtt gcagaacgtc ggtcgacagg gcggaaagat
     1561 cgtggtgcag accctcgagg ccaaaggcat gggcaatcgg ctgcaggcca aaggcagctg
     1621 ggatgagatc ccgcgcggac ccttcctggt cgagggcagt tcggaaattg atctggcgaa
     1681 gctgcaggcg cagctgccga tcgagaatcg tgagttcaag gagaaagcca gactggcagg
     1741 gcaagcgaat gtcgctctca agctctcagg aacgactgag gctccgaaac tggctggtac
     1801 tgttaaactg gaaaaggctc tgtttcatct gccggaacga gggttggcat ttgatgccat
     1861 caacggaagt gtccgagctg atatgcagtc agttgctgtc gaaaagctcg aagcgacggt
     1921 tgccggcgcg aaaatgtcgc tgggtggcga gctgaaaaac ttcacgaagc ctcagttgca
     1981 gctgcgcgcc gcgctcacgg ggctggatat gtccgaggtg cgaacgtttc ttttgcgcaa
     2041 tatcagcggg ttcccggccg atcttgattt ttccggaggc acggatatcg aggtacaggt
     2101 cagcgggtcg cccgaaagcc cgaagatcag cggttccgcg attcttgccg gcgcgcgtct
     2161 gtcacacccg gtcctgtttc ggccgattca gcagctggtt gggccacttg cgtttgataa
     2221 caagacgatc agcaccgaaa atctgacctt cagttggggc agttcgacgg cgcgcatcaa
     2281 ggggaaggtc gatgatatct cgaagtttgc tctgaatttt tcgtatgtgg ttgatcccct
     2341 cgatctcacc gatatcggaa cgtttttcct ggcgagtaca ggctataaag catcgggaat
     2401 gggaactggt cagggaaaga ttcatggtcc gatcgaaaag ctggtgatcg atggcaccgc
     2461 aattgtcccc gttggaatgt tcgaagcccc gctttcgaag ggcagtgcat cg
//
LOCUS       DAORHD010000736         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50230_length_2506_cov_4.267646, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000736 DAORHD010000000
VERSION     DAORHD010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50230_length_2506_cov_4.267646"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PKO06_14730"
     CDS             complement(<1..1155)
                     /locus_tag="PKO06_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70954.1"
                     /translation="MAKTSKKVREWKPQPQPLLTFWQKSFLVGILLFIPFFLLVMLPL
                     IARNHIVKNMKIYEDQLFAMPETINLVRTDLEPAFFKLKIDHLFIRVPRNMTPVRIEP
                     NAAVFRANPRRISRTLAVAAHPDAAKVFEPSLGLLRFFMPRKPLDYLDAALHSDYHPV
                     RQLCKAYFLAGQGVSGKVFKAEWDIHHRGYIVPNPGQTGWIGRVFCEEGDGMSEVSLY
                     DESVAATLRDWVNIALTVHPHASPTLSIENAPIKPLMDQAVRDEKAQNQVIETCLNEY
                     YANHNPKWSIPLAVVLQRRGYLREVIEMGNYFSPLVKTDVDLAPLWQEIIDQTMTHFI
                     SIELDLHPKLNRLRLKCRNKTDLPLRNVKLQVTVKTSKGERQFPVLLFDQG"
     gene            complement(1161..2327)
                     /locus_tag="PKO06_14735"
     CDS             complement(1161..2327)
                     /locus_tag="PKO06_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV70955.1"
                     /translation="MTRRSLLIVACCFWLASAAASLHGESAGSPEGKRLALDGIGTVM
                     IRNTSGGTEGEISQLLLEKPGGSLQIVATQEVMDFVDLMKGDLDGDGQQEVIAVARNR
                     AGDDVMPFIFRPGNTFHQVFPEFEEENPIIGKEISLIPGSGKSFMAVRVLVNVHDFGP
                     PDLFTVESYALQGKKLIKSGEKLLEGTHFNQVLNRAGLQFQRGEYLKALEGYQSLIAS
                     HAASMPTQACVVAMLYEAESRKFLKDFTGALPLYQKVFTTFPSSPEAESAKREYEFIS
                     KNLQSSKALSLFIDASQLESTNRLTEALQLIEQTRPQYEQGPLADHMLFLQSEILVSL
                     GRAPEAMTALEQLCRQFPQSALLPRAQELIQELQGNPDETSDLNGTPDHHNQDN"
     gene            complement(2324..>2506)
                     /locus_tag="PKO06_14740"
     CDS             complement(2324..>2506)
                     /locus_tag="PKO06_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70956.1"
                     /translation="PVVTHAPGTHEVAMGGGGHLAASGCNRAAPLMDVANEALHHLRE
                     RLATFTSAARHAEQNP"
BASE COUNT          516 a          668 c          774 g          548 t
ORIGIN      
        1 cccctgatcg aagagaagca cgggaaactg ccgttcccct ttggacgtct tcacggtgac
       61 ctgcagcttg acgttgcgca aaggaagatc ggttttgttg cggcacttga ggcgcaatcg
      121 attcagtttc ggatgcagat cgagttcgat actgatgaag tgcgtcatgg tctgatcgat
      181 gatctcctgc cagagagggg cgagatcgac atcggtcttg acgagcgggc tgaaatagtt
      241 gcccatttcg atcacttcgc gcagataccc acgccgctgc agaacgacgg cgagcggaat
      301 cgaccacttt ggattgtggt tggcatagta ttcgttgagg caggtctcga tgacctgatt
      361 ctgggctttc tcatcccgca ctgcttgatc catgagtggc ttgatcggcg cgttttcgat
      421 cgagagggtc ggcgaggcat gcgggtgaac ggtcaaggca atgttcaccc agtcccgtag
      481 cgtggccgcg accgattcat cgtagagaga aacttcgctc atgccgtcgc cttcttcgca
      541 gaagacacgc ccgatccagc ctgtctggcc gggattcggc acgatgtacc cgcggtggtg
      601 gatatcccac tccgctttaa agaccttgcc gctcacgccc tggccagcta gaaaatacgc
      661 cttgcacagc tgccggacgg gatggtaatc ggaatgcagg gcggcatcga gatagtcgag
      721 tggttttcgg ggcatgaaga agcgcagtaa cccgagcgat ggttcgaaca ccttggcggc
      781 atcggggtgc gcggccacgg ccagcgtgcg cgaaatgcgt cgcggattgg cacggaagac
      841 ggcggcgttg ggctcgatac gcaccggagt catgttgcgg ggcacacgga tgaacagatg
      901 gtcgatcttc agcttgaaga aggcgggttc aagatcggtg cgcaccaagt tgatcgtctc
      961 gggcatcgca aaaagctgat cttcgtagat cttcatgttt ttgacgatgt ggttgcgcgc
     1021 gatcagaggc agcatgacca gaagaaagaa cggaatgaac agaaggatac cgacgaggaa
     1081 agacttttgc cagaaggtca gaagcggctg cggttgcggt ttccactccc ggactttctt
     1141 gctggttttg gccatgccga ctaattgtcc tggttgtgat ggtcaggagt gccgttcaaa
     1201 tcagaagttt cgtcgggatt gccctgcagt tcctgtatca gttcctgtgc ccggggcaac
     1261 agggcgctct gggggaactg tcggcagagc tgttcgagcg cggtcatggc ttcgggggcg
     1321 cgcccgagag acacgagaat ctcgctctgg agaaacagca tgtggtcggc cagcggcccc
     1381 tgctcatatt gaggtcgtgt ctgttcgatc agttgcaacg cctcggtcag ccgattcgtc
     1441 gattcgagct gcgaggcatc gataaagagc gacaatgctt tgctggactg cagatttttc
     1501 gagatgaact cgtactctct tttggcgctc tcggcctcgg ggctgctcgg aaacgtcgtg
     1561 aaaacctttt gatacagggg cagggcaccg gtgaaatctt tcagaaattt gcgcgattcc
     1621 gcttcataga gcatggcgac aacacaggcc tgcgtcggca tactcgccgc atgagaggcg
     1681 atgagggact gatagccttc gagggctttc agatactcgc cgcgctgaaa ctgcaacccg
     1741 gcacgattga gaacctggtt gaaatgcgtg ccttcgagca acttctcgcc gctcttgata
     1801 agctttttcc cttgtagcgc ataggactct acggtgaaca ggtctggagg tccgaagtcg
     1861 tgcacgttca ccagcacacg cactgccatg aaagacttgc cgcttccggg aatgaggctg
     1921 atttccttgc cgatgatcgg gttctcttct tcgaactcgg gaaaaacctg gtgaaaagtg
     1981 ttgcccggtc gaaagatgaa gggcatcacg tcatcgccgg ctcgattgcg ggctacggca
     2041 atgacttcct gttgcccatc tccgtcaaga tcgcccttca tcaggtcgac gaagtccatg
     2101 acctcctgag tggcgacgat ttgcagtgag ccacccggct tctcgagcaa caattgcgaa
     2161 atctcgccct cggtgccgcc gctggtgttg cgaatcatca ccgtgccgat gccgtcaagt
     2221 gccaggcgtt tgccctcggg actgccggcc gactcaccgt gcagcgaagc tgcggcggaa
     2281 gcaagccaga aacaacaggc gacaatcagc agggagcgtc gcgtcatggg ttttgttccg
     2341 cgtgtcgggc agcggatgta aaagtggcga gtcgttcacg cagatgatgc agagcttcgt
     2401 tggcgacatc catcagcggt gcggcacggt tacagccgga agccgccagg tgtccgccgc
     2461 cgcccatggc tacctcgtgc gtcccgggag cgtgggtgac aactgg
//
LOCUS       DAORHD010000737         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50240_length_2506_cov_3.572011, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000737 DAORHD010000000
VERSION     DAORHD010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50240_length_2506_cov_3.572011"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="PKO06_14745"
     CDS             <1..601
                     /locus_tag="PKO06_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70957.1"
                     /translation="GSFANSGAARLFLGLLLIFSWSVRAQAAAERSSGEILFLTGAVL
                     LDGVDLASGTLRLPVDRSFDLKTGEDGQIRFVGPGREDTRLRERSHLVWNAGDQSWSV
                     RSGLCGFWRRAQQPGSASDARRISVPQGECLMQSGILVVKAKASLSRVAAVKGAHQLL
                     GPEGECLALDEKKERAVAWKQTSQAYEVTDDCYYAWYWK"
     gene            617..1312
                     /gene="queC"
                     /locus_tag="PKO06_14750"
     CDS             617..1312
                     /gene="queC"
                     /locus_tag="PKO06_14750"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNV70958.1"
                     /translation="MKPKAIVLLSGGLDSTTCLAIAIKQGFEPYALSFRYGQRHETEL
                     AAARRVAQALNVARHVVIDFNLREFGGSALTADIPVPKHRADAEIGDGVPVTYVPARN
                     TIFLSFALGWAEVLGAFDLFIGVNALDYSGYPDCRPQFIEQFAKLAQVATKAGAEGSG
                     TYRIHAPLLQMTKAQIVRAGIELGVDYSLTFTCYDPLPSGESCGACDSCLLRLKGFAE
                     AGVTDPIPYRLTR"
     gene            1319..1966
                     /gene="queE"
                     /locus_tag="PKO06_14755"
     CDS             1319..1966
                     /gene="queE"
                     /locus_tag="PKO06_14755"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HNV70959.1"
                     /translation="MSYRIKEIFYSLQGEGALTGRAAVFCRFAGCNLWSGRDEDRAIA
                     ACPFCDTDFVGTNGDGGGEFESPEALADFVSSVYRGDRLLIPPMVILTGGEPLLQVDR
                     PLLQALHHRQFLIACETNGTIAAPEGIDWLTVSPKAGTRIIQQSGSELKVVFPQAEVE
                     LHLFAGWSFQHRFVQPRYAVDAEQYRQNLDAAIEWCLSNPAWRLSLQTHRHTGIR"
     gene            2057..>2506
                     /locus_tag="PKO06_14760"
     CDS             2057..>2506
                     /locus_tag="PKO06_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70960.1"
                     /translation="MANYRNGRTWFIGFALCCLLLVGPTAHAFNLNNFLDEANHALDK
                     FSGVVDKTFGISGSTPPTVPASTGQAQAPAPAETAGNSAQAASGGSWWDRIFGGVENS
                     TEKTMGAQGHEALKKSPGFYNNANDLNRVGTIARKLVKVVDRKDLTYR"
BASE COUNT          470 a          693 c          778 g          565 t
ORIGIN      
        1 gggcagcttt gcgaacagcg gagctgcccg tttgtttctc ggtctgctgc tgatcttctc
       61 gtggtcggtt cgtgcccagg cagccgcgga gcgcagttca ggggaaatcc tcttcctcac
      121 tggtgccgtg ttgctcgatg gggtcgactt ggcaagcggt accctgcgtc tgccggttga
      181 ccggtcgttc gatctgaaga ccggtgaaga tggtcagatc aggtttgtcg gcccgggacg
      241 ggaagacacg cgtctgcgtg agcggtctca tctcgtctgg aatgcgggtg atcaatcgtg
      301 gagtgtcaga agcggtttgt gtggcttctg gcgtcgggct caacagccgg ggtctgcatc
      361 tgatgccaga cgtatcagcg ttcctcaggg ggaatgcctc atgcagagcg gcattctggt
      421 cgtgaaggcc aaagcctccc tgagtcgggt ggcggcggtc aagggcgcgc atcagttgct
      481 tgggcctgaa ggcgagtgtc tggccctcga cgaaaaaaaa gagcgggcgg tggcctggaa
      541 acagacttca caagcctacg aggtaaccga tgattgctac tatgcctggt attggaagta
      601 gacgcggagg tcgttcatga aaccgaaagc gattgtgttg ctcagtggtg ggctcgattc
      661 gacgacctgt ctggcgatcg cgatcaaaca gggctttgag ccctacgcgc tttcgtttcg
      721 gtacggacag cgtcacgaaa ccgaactggc cgcggccagg cgggtggcgc aggccctgaa
      781 cgtcgctcga catgtggtga tcgatttcaa cctgcgcgag tttggcggat cagccttgac
      841 cgcggacatt cctgtgccga aacaccgtgc tgatgcggag attggcgatg gcgtccccgt
      901 cacgtacgtg ccggcgcgca acacgatttt tctctcgttc gcgctcggct gggccgaggt
      961 gcttggtgcc tttgatctct tcatcggggt caacgccctc gattacagcg gatatcccga
     1021 ttgtcgtccg cagttcatcg agcagttcgc gaagctggcg caggtggcga cgaaggcggg
     1081 tgccgaaggg tcgggaacct atcgcattca cgctccgctt ctgcagatga ccaaggctca
     1141 gattgtccga gctggcattg agctgggagt agattattcg ctgactttca catgctacga
     1201 tccacttccg agtggtgagt cctgtggggc gtgcgactcg tgtctcctgc gactgaaggg
     1261 gtttgccgaa gccggggtga cggatccgat tccgtaccgc ctcacgaggt agtttcgcgt
     1321 gtcgtatcgc atcaaggaaa tcttctattc gctgcagggt gagggcgcgc tgactggtcg
     1381 agccgcggtg ttctgtcggt ttgccgggtg caatctctgg tctgggcgcg atgaagatcg
     1441 agcgatagcg gcgtgtccct tctgcgacac cgattttgtg ggcactaatg gggacggagg
     1501 cggtgaattt gagtcgccgg aggccctcgc agatttcgtg tcgtcggtct accgaggcga
     1561 tcgcctgctg attccgccca tggtcattct cacgggtgga gagccgctgc ttcaggtcga
     1621 tcgaccgctg ctgcaggcgt tgcatcatcg ccagttcctc atagcctgcg agacgaacgg
     1681 aacgattgcc gctcccgaag gaattgactg gctgactgtg agccccaaag ccggcacgcg
     1741 catcattcaa cagagtggct cggaactcaa agtcgttttt cctcaggcgg aggtcgaact
     1801 gcatctgttc gccggatggt cctttcaaca ccggttcgtg caacctcggt atgcggttga
     1861 tgccgaacaa tatcgccaaa acctcgacgc tgccattgag tggtgtctct cgaaccctgc
     1921 ctggcgtctc agtttgcaga cccaccgcca taccggcatt cgttaacggc gccacccacc
     1981 tattgacggt acgcatttcc attcttatga tggagtaaat tcacgccgtt ggtggcgttt
     2041 tttggagatt ctgctcatgg cgaattatcg aaatggccga acctggttca ttggcttcgc
     2101 cctgtgttgt ctgcttctgg ttggaccaac cgctcacgcc ttcaacctga acaactttct
     2161 cgacgaggcg aatcacgccc tcgacaagtt cagcggtgtg gtcgacaaaa ccttcggaat
     2221 cagtggttcg acgcctccga ccgttcctgc ctcaaccggg caggcgcagg caccagcacc
     2281 ggcggaaaca gcgggaaact ccgctcaggc agcctcaggc ggcagttggt gggaccgcat
     2341 cttcggtggt gtcgagaact cgaccgaaaa aaccatggga gctcagggcc atgaagctct
     2401 caagaaaagc cccgggttct acaacaatgc caacgatctg aaccgcgttg gcaccattgc
     2461 ccgcaaactg gtaaaggttg tcgatcgcaa agacctgacc tatcgg
//
LOCUS       DAORHD010000738         2505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50262_length_2505_cov_3.676327, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000738 DAORHD010000000
VERSION     DAORHD010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50262_length_2505_cov_3.676327"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKO06_14765"
     CDS             complement(<1..985)
                     /locus_tag="PKO06_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70961.1"
                     /translation="MKMIKTTREVPFLLTVAALFVVAIVHLGLQIWGSPAHRTPADAL
                     EAPVKAPKISSPTIDPEPAPPETTPPATESPEEPAAEPESQPVPDAEPEPSQPAPESG
                     DPVPAANPEPAPAPETSPEPKAELKTPKTEPKPVAKPAEAAKPAAVKKPAPKTEKVVA
                     KPADPSEVKVVKAEPLKPAKKGSRKKKKADVEPQASDYTVPAEWNWFSKPLTMKHNGS
                     EWVLAAEDGEAGEPLQEAKQEPAASDVAAMADVPASDEPEQVEQAASVTEPVAVPAPI
                     SNTTQESAVIAPEKARSTIVSEGVVALPPFERALAKMASRKAKRLAEAERLS"
     gene            complement(1075..1641)
                     /gene="efp"
                     /locus_tag="PKO06_14770"
     CDS             complement(1075..1641)
                     /gene="efp"
                     /locus_tag="PKO06_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV70962.1"
                     /translation="MPIIANDLRKGMIILFNNELCQVVEVDFVRPGNWRAMAQTKLRS
                     IKTGAIFLQRFGTTEKLDEAAVETRNMQYLYCADGMYFFMDHQTYEQLEITEDFIGEA
                     KKFLKEQMDVVVKLYEGRPIGLDLPSSVTLRVVECPPNIKGNTASGGSKPATCEGGHV
                     CQVPFFVEMGELIRVDTRTGDYLERAGK"
     gene            1894..>2505
                     /locus_tag="PKO06_14775"
     CDS             1894..>2505
                     /locus_tag="PKO06_14775"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV70963.1"
                     /translation="MDQSIVVSALLGAIQGLTEFLPVSSSGHLTIFQHLFGLQDPESN
                     LALDILLHSATLLAVIWFFRADLWVFATADGRARPENRRLALLVLVASIPTAVIGLGF
                     KKKFEALFGAPLIVCCALVATGVLLLAAHWQARRQAGGGIGELSLGHALFIGILQGIA
                     ITPGISRSGSTIAAGIFSGMKGEEAGRFSFLLMIVSVGGATLLE"
BASE COUNT          514 a          715 c          654 g          622 t
ORIGIN      
        1 cagagagtcg ttcagcttca gccagtcgct tggctttccg agaggccatt ttcgccaagg
       61 ctcgctcaaa cggaggcaac gcaacaaccc cttcggagac tatcgttgaa cgagctttct
      121 caggggcaat caccgcagat tcctgcgtcg tgttcgagat tggggcgggc acggctaccg
      181 gctcggttac ggacgctgcc tgctctacct gttccggctc atcggaagcc gggacatcgg
      241 ccatagcagc cacgtcgctt gccgcgggct cctgtttcgc ttcctgcagg ggttcgcctg
      301 cctctccatc ttctgccgcc agaacccatt ccgagccgtt atgcttcatg gtcagaggct
      361 tggaaaacca gttccattca gcgggaacgg tatagtcaga agcctggggc tcgacatcag
      421 ccttcttctt cttccgggaa cctttcttcg ctggcttcaa tggctcagct ttcacaacct
      481 tgacttccga ggggtctgct ggcttggcaa cgaccttttc ggtctttggt gccggctttt
      541 tcacggcagc gggtttagcc gcctcagccg gcttggcgac cggtttcggc tccgtttttg
      601 gggttttcag ctcagcctta ggttcaggac tcgtttcggg agcaggagcg ggttcgggat
      661 ttgcagcggg cacgggatcg ccagactcgg gagccggctg actgggctct ggttctgcat
      721 cgggtactgg ctgcgactct ggctctgccg ccggttcttc ggggctttcc gtggcgggcg
      781 gagtcgtctc aggtggagcg ggttccgggt cgatggtcgg cgaagaaatc ttgggcgctt
      841 ttaccggcgc ttcaagagca tcagcaggcg tacgatgggc aggtgatccc catatttgaa
      901 gacccaagtg gacaatagca accacaaaca gtgccgccac tgtcagaagg aacggaacct
      961 cacgagtcgt ttttatcatt ttcatgatta tcaaccccta atatctctgc caccgtccgt
     1021 tcacgtacta ttcggacaat ctaccgtcac gcgagcgaaa agcaaggaga tcgattactt
     1081 tcctgcgcgc tccagatagt cgcccgttcg cgtatcaacg cgaatcaatt cgcccatttc
     1141 cacgaagaat ggaacctgac agacgtgtcc gccctcacag gtggccggtt tggaaccacc
     1201 actggccgtg ttacccttga tgttcggggg gcattcgacc acgcgaagcg ttacagaaga
     1261 cggaagatca agaccgatcg gccgcccttc atacaacttc acgacgacat ccatctgttc
     1321 tttcaggaac ttcttcgcct caccgatgaa gtcttctgtg atctcgagtt gttcataggt
     1381 ctgatggtcc atgaaaaagt acataccgtc ggcgcaatag agatactgca tatttcgcgt
     1441 ttctacggcc gcttcatcga gtttctcggt cgttccgaaa cgctggagaa agatcgcccc
     1501 cgtcttgatg ctgcgcaatt ttgtctgagc catcgcacgc cagtttccgg gacgcacgaa
     1561 atcgacctcg acaacctggc acaattcgtt gttgaacaga ataatcatac ctttgcgcaa
     1621 atcattcgcg atgatgggca tttctgactc ctcggggtct gagtatcgac agaatatacc
     1681 acatttttca cattttccaa aacagaaaaa tgattttctg cgtaacggcc aacaaaagta
     1741 gacctagaat cgaacgttaa tatataatta caaacatcaa tatcaaaata ttaatttcaa
     1801 taaaattttg atcatttgaa atgttctcgc gtttaaaaga ccgcctatga gatgccgata
     1861 aggcatagag atatacattc tgaggcaaac accatggacc aatcgatcgt tgtatctgcg
     1921 cttctcgggg caattcaagg cctcaccgag tttctccccg tgtcaagttc ggggcatctg
     1981 accattttcc aacacctgtt cggactccag gaccccgaat ccaatcttgc gctcgatatt
     2041 ctcctgcact cagccactct cctggccgtg atctggtttt tccgcgccga cctgtgggta
     2101 ttcgccaccg ccgatggccg cgcccggccc gagaatcgcc ggctcgcgtt gctggtgctt
     2161 gttgccagca tacccacggc ggtgatcgga ctcggcttca agaaaaagtt tgaagccctt
     2221 tttggcgcgc ctctgatcgt ctgctgcgcc cttgtcgcca cgggcgtcct tctgctcgct
     2281 gcccactggc aagcgcgccg gcaagccgga ggaggaatcg gtgagttatc acttggtcac
     2341 gcactcttca tcggaattct gcagggaata gcgatcactc cgggcatttc gcgctccggt
     2401 tcgacgatcg ccgccggaat tttttccggg atgaaaggcg aggaagcggg tcgtttctcg
     2461 tttcttctca tgatcgtctc cgtcggagga gccacgctgc tcgag
//
LOCUS       DAORHD010000739         2496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50549_length_2496_cov_4.000410, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000739 DAORHD010000000
VERSION     DAORHD010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50549_length_2496_cov_4.000410"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2496
                     /locus_tag="PKO06_14780"
     CDS             <1..>2496
                     /locus_tag="PKO06_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HNV70964.1"
                     /translation="YHQPRHGVVDRVASPYGCFIRRQHFDLSGLDVDPTWLKDLDPLF
                     PLVLHVGRDCWQSAKTANIDRQRTGVFIANIVLPTDATMNLTRRLYGPQVAKHLGTSL
                     RHDGGAFEMFASPRNVHAAGLPAGLLARALGLGGGCMTLDAACASSLYAIKLACEELV
                     SGRLDAVMTGGVGRASSLFTQMGFSQLRALSPSGVCRPFDAGADGLVVGEGAGFFLLK
                     RLDDAMRDGDHIHVVIRGIGLSNDVGGSLLAPDSEGQLRAMRDAYRQAGWSPDDVDLV
                     ECHGTGTPTGDRVETASLQELWKEIPGKPGRCVIGSVKSNVGHLLTGAGAAGFMKVLL
                     ALREKTLPPVANFRQSDASMRLENGPFRVLSKPEPWPVREPDRPRRAAVSAFGFGGIN
                     AHVLVEEWLTPETTSCAHAAEGSGFPSSSSSLQADWRAQSPVQIDSLVDEPIAIVGMD
                     ACFGRWADLSSFQEAVLSGRAEAGTKPAGRWATDLAVGAPAGEYLADLSFRLGQFRIS
                     PNELADMLPQQLLMLQVVARALERVVGTKPPALRWSVFTGIGLDLNSTNFALRWALAA
                     DVNTKMSANDLDSRLDLVSRPLTANRTVGALGGMVASRIAREFHVGGPSHTVSSEETS
                     GLRALETARRALQRGEVDLAIVGAVDLVGDPRLLWAMRQERSAMQTEPGVPLDESAPG
                     WKPGEGAAAVILKRLSDAQRDGDRVYCIIKGVGAACGGTTAETRPPAAAYRQSLDRAL
                     ADAGVAAHRLGYYEAHGSGIPEEDRVEAEVLGEFFADAVPAARELRRYEGPFCALGSA
                     KPVVGHTGCASGLVSLVKTALSVYHELLPGFHGL"
BASE COUNT          426 a          709 c          841 g          520 t
ORIGIN      
        1 taccatcaac ccagacacgg agtggtcgat cgggtggcgt ccccctacgg ctgtttcatt
       61 cgccgccagc actttgatct gagtggtctg gatgtcgatc cgacgtggtt gaaagacctc
      121 gatcccctgt ttccgttagt gctgcatgtg gggcgtgact gctggcagtc agcgaagacc
      181 gcaaacattg atcgacagcg cacgggagtg ttcatcgcca acattgtgct gccgaccgat
      241 gccaccatga atctgactcg tcgactgtac gggccacagg tggcgaagca ccttggaacc
      301 tcattgcgac acgatggggg cgcattcgag atgttcgcca gcccgcgaaa cgtgcatgcc
      361 gccgggctgc ccgcgggcct gctggcacgt gccctgggtc tcggtggtgg ctgcatgacg
      421 ctggatgccg cgtgcgccag ttcgctctat gcgatcaaac tcgcctgcga agaactggtt
      481 tcagggcggc tcgatgccgt catgacgggc ggtgttggtc gcgcgtcctc gctgtttacg
      541 cagatgggat tcagccagtt gcgagcgctc tcgccttcgg gagtctgtcg gccttttgat
      601 gcgggtgccg acgggcttgt cgttggagag ggggcaggat tctttctgct gaagcggctc
      661 gatgatgcca tgcgcgacgg cgaccacatt catgtggtca ttcgcgggat cggcctgtcg
      721 aacgatgtcg gcggaagcct gctggctccg gattctgaag ggcaactccg cgccatgcgt
      781 gatgcctatc ggcaggcggg ctggagcccg gacgacgtcg atctggtcga gtgtcacggc
      841 acgggaacgc cgacggggga ccgggtcgaa accgccagtc tccaggagct ctggaaagaa
      901 attcctggaa agcccggtcg ttgcgtgatc gggtcggtga agtccaatgt gggccatctg
      961 ctgaccggag ccggagccgc cggtttcatg aaagtgctgc tggctttgcg tgagaagact
     1021 ctgcctcccg tcgcgaattt ccgccaatcc gatgcctcga tgcgtctcga gaacggtccg
     1081 tttcgggtgc tctcaaaacc agaaccgtgg cctgttcgcg aacccgatcg cccacgtcgg
     1141 gccgccgtca gcgcgttcgg cttcggcgga atcaacgctc atgtcctcgt cgaggagtgg
     1201 ctgactccgg aaacgacgtc atgcgctcat gctgccgaag gatctggatt cccttcttcg
     1261 tcgtcgtcgc tgcaggcgga ttggcgcgcg cagtcaccgg tacagatcga cagtctggta
     1321 gacgagccga ttgcgattgt cggcatggat gcctgcttcg ggcgatgggc tgacctgtct
     1381 tcgtttcagg aagcggtgct gagcggtcgg gcagaggctg gcaccaagcc tgccggtcga
     1441 tgggccactg atctggccgt gggtgcgcct gccggcgagt atcttgccga tctctcgttc
     1501 agacttggac agtttcgcat ttctccgaac gaactggccg atatgttgcc gcagcagtta
     1561 ttgatgctgc aggtcgtcgc tcgagccctt gaacgcgtgg ttggcacgaa gccgccggcc
     1621 ttgcgatggt cggtcttcac cggtatcggg ctcgatctca atagtacgaa ttttgccttg
     1681 cgttgggcgc tggcagctga tgtaaacaca aaaatgtctg ccaatgacct ggacagtcga
     1741 ctcgatcttg tcagccgacc cctgacggcg aatcgcacgg tcggagcact ggggggaatg
     1801 gtcgcaagtc gaatcgcccg tgagtttcat gtcggagggc cgtcgcacac ggtttccagc
     1861 gaagagactt ccggtctgcg ggcacttgag accgcacgcc gcgcactgca acgcggtgag
     1921 gtcgatctgg cgatcgtggg ggcggttgac ctggtaggag atccgcggtt gttgtgggcc
     1981 atgcgccagg aacgctcggc catgcagacg gaacccggtg ttcctctcga cgagagcgca
     2041 ccgggatgga aaccgggcga gggagccgcg gcagtcattc tgaagcgact cagcgatgcg
     2101 caacgcgacg gtgaccgggt atactgcatc atcaagggag tgggtgcggc gtgcggtggt
     2161 acaactgctg aaacaagacc gccggccgcg gcgtatcggc agtcgctcga tcgtgctctt
     2221 gccgacgcgg gggtggccgc gcaccgactc ggctactacg aggcgcacgg cagcggtatt
     2281 cccgaggaag atcgggtcga ggccgaagtg cttggcgagt tttttgccga tgccgttccc
     2341 gccgcgagag aactgcgtcg atacgaaggg ccgttctgcg cgctggggag tgccaagccg
     2401 gtcgtgggtc acacgggctg cgcttcaggt ctggtttctc tggtcaaaac ggcattgagt
     2461 gtgtatcacg aactgttgcc agggttccac ggtttg
//
LOCUS       DAORHD010000740         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50611_length_2494_cov_4.115621, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000740 DAORHD010000000
VERSION     DAORHD010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50611_length_2494_cov_4.115621"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKO06_14785"
     CDS             <1..493
                     /locus_tag="PKO06_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70965.1"
                     /translation="VVLKKMLYATGLINAAYLGMNDLVAALQMAKKSDDPKMISAQLL
                     AVRLGLDAKPALLRHIAQEIRQCEAYPKFPLGFNDGPEGPELISDEFEKMAFAIENLN
                     IQPHLTTLAALRKEAERVVFAIFWTESEKMRALAHDIHTFLRVELKEDLPFDVFTKPR
                     SSR"
     gene            complement(586..>2494)
                     /gene="uvrB"
                     /locus_tag="PKO06_14790"
     CDS             complement(586..>2494)
                     /gene="uvrB"
                     /locus_tag="PKO06_14790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV70966.1"
                     /translation="EFRDFFPHNAVEYFVSYYDYYQPEAYLPGQDLYIEKDSDINEEI
                     DRFRHAATSSLFTRRDVIVVASVSCIYGLGSPQDYAGLRISLRLGETLPRQELLRKLI
                     EIQYSRNDMDFHRSRFRARGETIDIFPAASEDAIRVKFWGDDIERLERFDPLTGEVVA
                     SYEAVDIFPAQHYVTPEAKRERAIAAIEEELKDRVTEFEKAKKLIEAQRVFERTRYDL
                     EMMRETGFCKGIENYSRHLAGRAAGAPPETLVDYLPRDCVIIIDESHMTVPQIRGMSH
                     GDRSRKQTLVEFGFRLPSALDNRPLTFDEFLAHQRQTLFVSATPGKWELEVSGDAVAE
                     QLIRPTGLVDPQIMVVPTNGQIDHLISRIRERIDANERVLVTTLTKRMSQELATYLST
                     AGILTRYLHDEIETLERIQILRELRLGVFQVLVGVNLLREGLDLPEVSLVAILDADKE
                     GFLRSAWALMQTAGRAARHLHGEVIMYADKITDSMQYCIDETARRRRIQEAFNLENGI
                     KPASIVKELPKAFMPGAGAEAEAGEGRQLSPDAVKSTIAELQKEMQKAAESMQFERAA
                     LIRDEILALQEEYLEADILSGLGQALRKGKESGVNRKQALKRRSPTPPLAADRASSSP
                     HTRRPRGGRRN"
BASE COUNT          487 a          712 c          755 g          540 t
ORIGIN      
        1 cgtcgtcctc aagaaaatgt tgtatgccac gggcttgatc aatgccgcct atctgggcat
       61 gaatgacctg gttgccgcgt tacagatggc caagaagtca gatgacccca agatgatctc
      121 ggcacagctg ctggctgttc gtctcgggct cgatgccaag ccggcactgt tgcggcacat
      181 tgcgcaggag atcaggcagt gtgaggcgta cccgaagttt ccgctcgggt tcaatgacgg
      241 cccggaaggc cccgagttga tcagcgatga gtttgaaaaa atggcgtttg cgattgagaa
      301 tttgaatatt caaccgcatc tgacgacgct ggccgctttg cggaaagagg cggagcgcgt
      361 tgtgttcgcc atcttctgga ccgaatccga aaagatgcgc gctctggctc acgatattca
      421 cacctttctg cgtgtcgaac tgaaagaaga tctccccttc gacgttttca ccaagccgag
      481 atccagccgc tgattcatca ccggacccgg gacttctcaa agacgcccca gattaccgcg
      541 gcgacaacga ggggcaggat gtttccgttt ttctgttgcc cgcgatcaat ttcgtcttcc
      601 gccccgtggc cgacgggtgt gggggcttga ggaagcgcgg tcggcagcga gcggaggagt
      661 gggggaccgt cgtttcaggg cctgtttccg gttgacgccg ctttctttcc ccttgcgcag
      721 ggcctgaccg agaccgctca ggatatcggc ttcgagatac tcctcctgca gagccagaat
      781 ctcgtcgcgg atcagggcgg cgcgctcgaa ctgcattgat tcagccgctt tctgcatctc
      841 tttctgcagt tcggcgatcg tcgatttcac cgcgtcaggc gagagctgac gtccctcgcc
      901 ggcttccgct tcagcgccgg ccccgggcat gaaggctttt ggcagctcct tgacgattga
      961 tgccggtttg atgccgttct cgaggttgaa ggcctcctga atgcggcgac gtcgagcggt
     1021 ctcgtcgatg cagtactgca tcgaatcggt gatcttgtcg gcatacatga tcacttcgcc
     1081 atgcagatgt cgcgcggctc gaccggcggt ttgcatcagg gcccaggcgg aacgcagaaa
     1141 tccttccttg tccgcgtcca ggatggcgac gagtgaaacc tcgggcagat caagaccctc
     1201 gcgcagcaga ttcacgccaa cgagcacctg aaaaactccg agccgcagtt cccgcagaat
     1261 ctgaatgcgt tcgagcgttt cgatctcgtc gtgcagatag cgcgtgagaa tgcctgccgt
     1321 gctgagatac gttgccagtt cctgggacat tcgcttggtc agtgtcgtga caagaacgcg
     1381 ctcgttggca tcgatgcgct ctcgaatgcg gctgatcaga tgatctatct gaccattggt
     1441 tggtacaacc atgatctgcg gatcgacgag cccggtcgga cgaatcagct gttcggccac
     1501 ggcgtcaccc gacacctcga gctcccattt ccctggagtg gcggaaacga acagggtctg
     1561 gcgctggtga gccagaaact cgtcgaaggt cagcggccgg ttgtcgaggg ccgaaggcag
     1621 gcggaaaccg aattcgacca gcgtctgctt acgactgcga tcaccgtgcg acatacctct
     1681 gatctgcggc accgtcatgt gggactcatc aatgatgatg acgcagtcgc gtggcagata
     1741 atcgacgagc gtctcgggcg gcgctccggc cgcgcgcccc gccagatgcc gggagtaatt
     1801 ttcgattcct ttgcagaaac cggtctcgcg catcatctcg agatcgtacc gggttcgttc
     1861 gaagacgcgt tgtgcttcga tcagcttttt cgccttctcg aactcggtga cgcgatcttt
     1921 caattcctct tcgatggccg cgatggcccg ctcgcgcttg gcttctggcg tgacgtagtg
     1981 ctgcgcggga aagatgtcga cggcttcata ggaagccacc acttctccgg tgagcgggtc
     2041 gaagcgctcg agtcgttcga tgtcgtcgcc ccagaacttg accctgatgg catcttcgct
     2101 ggcggctggg aagatgtcga tggtctcacc ccgggcgcga aagcgcgaac ggtgaaagtc
     2161 catgtcattg cgcgaatact ggatctcgat caacttgcgc agcagctcct gacggggcag
     2221 ggtttcaccg agccgaagcg agatgcgcag cccggcgtaa tcctgcggag aaccgagacc
     2281 gtagatacag ctgaccgaag cgacgacgat gacgtcgcgc cgggtaaaca gcgagctggt
     2341 ggccgcatga cgaaagcggt cgatctcttc gttgatatcc gaatccttct cgatgtacag
     2401 gtcctggccc gggagatagg cttccggctg gtagtagtcg tagtagctca cgaagtactc
     2461 gacggcattg tgcggaaaaa agtcgcgaaa ctcg
//
LOCUS       DAORHD010000741         2492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50674_length_2492_cov_3.279032, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000741 DAORHD010000000
VERSION     DAORHD010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50674_length_2492_cov_3.279032"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2492
                     /locus_tag="PKO06_14795"
     CDS             <1..>2492
                     /locus_tag="PKO06_14795"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HNV70967.1"
                     /translation="SALTAYWISAQAQVELITLHYLVLFAIPGYHVVLHQIVRRQARE
                     HSMIESASHLSPLLAMGLVGILLYHPATAAAPIPSFLFLVLLNILNGWHTLKSERALP
                     FYHLSGIANFALIWLWSARHLSAEHIPLSFCLFAGFSLYYLILGELGPRFSSAHPDIR
                     IPAACFPLFACTFVFALIDQAPAGMNPVSPLWLLLLLDAFVAFQAVRDDQARPIHFIG
                     GIVSFLLLMKWNQLLLSDITLPWALFFFAVFAISHAAVPVLLQRLRPESSPYLWGHLF
                     PPLMLLLMVFPIIRGMAFSLLFWPVIFLVNIAGLIAAWLMAWVWAGVTFLLISMGLVT
                     LCLHRVTDTGHLAHVLVIEFGFSLLFHVWGMFVWKNRDRLVFHLGGWLKGFAGLLTRI
                     EPAEDQTDSPPDRRDVWESDPLNLAVLPSLSGLLPFVLVIFTLQRLPMPHPGQIFLFC
                     FAISILLLRLCKDAGTDATATIALFSVGLVELAWHQVNFSAANALDAAVWYIGFWLLF
                     LLFPFFFRDRVQQRALPWINAALAGPLQFSFLYLSYLELTGKTLPGILPALMALPYLY
                     GLKCLHACDGFPVPVKNQLLAWFGGTSLFFITLIFPIQFDREWLTIGWALLGASLVWL
                     YQRVPHEGLKHWGIGLLLISFARLADPAVFSYCPRQPTPLINWFLYTFSVTGASLITG
                     ATLLRPPHHEWFGVNIRRILYILGAVLFFFLLNIEIADFYSTGTTIVFQFSGEALERN
                     TAYSVGWSIFAFILLLVGIACNSRGARLGSIGLLTVTIFKVFLLDLWSLGGLYRVGSL
                     VGLAGALIMVSFLYQKFVTVRAPVSREPESPH"
BASE COUNT          397 a          763 c          588 g          744 t
ORIGIN      
        1 ttccgcgttg accgcctact ggatctcagc tcaggcccag gttgaactga tcactcttca
       61 ctatcttgtt ctgttcgcca tcccgggcta tcacgtcgtg ctgcaccaga ttgtgcgccg
      121 gcaggcccga gaacattcca tgatcgagtc ggcctcccac ctgtctcctc tgcttgctat
      181 gggcctggtg ggtatactcc tgtatcatcc ggcaacggcg gcagcaccga tcccatcctt
      241 tctgttcctc gttctcttga atatcctgaa cgggtggcac acgctcaagt ctgaacgagc
      301 acttccgttc taccatcttt cggggatcgc gaacttcgcc ttgatctggc tctggagcgc
      361 aaggcacctg agtgccgagc acattcctct ttctttctgc ctgtttgccg gtttcagctt
      421 gtactatttg atcctcggag aattgggtcc acgtttttca tcagcacacc cggacattcg
      481 catcccggcc gcgtgctttc cgctcttcgc ctgtacgttt gtgttcgcac tgatcgacca
      541 ggctccagcc ggcatgaatc cggtttctcc actgtggttg cttctgcttc tcgatgcctt
      601 tgtcgccttt caggccgttc gcgatgatca ggctcgacca attcacttca tcggcggcat
      661 cgtcagtttt cttctcctga tgaagtggaa ccagctcctt ctgtctgaca tcacgctccc
      721 atgggccctt ttcttttttg ccgtttttgc gatttcccac gcggcagttc ccgttcttct
      781 gcagcgcttg cgcccggaat catctcccta tctgtggggg catctgtttc caccgctgat
      841 gctgctcttg atggtctttc cgatcattcg cggcatggct ttttcgctcc tgttctggcc
      901 ggtgatcttc ctggtgaaca ttgccggctt gattgcagcc tggctcatgg cttgggtttg
      961 ggctggtgtc acctttctgc tcatttccat gggcctggtc accctgtgcc tgcatcgtgt
     1021 cacagatact gggcacttgg cacatgtact ggttatcgag tttggattct cacttctttt
     1081 ccatgtctgg ggcatgttcg tctggaaaaa tcgtgaccga ctggtctttc acctcggagg
     1141 ctggctaaag ggattcgccg gactgctcac ccggattgaa ccagccgagg atcaaacaga
     1201 tagcccaccc gaccgacgag acgtctggga atcagatcct ctcaatctcg ccgtgcttcc
     1261 ctcgctttcc ggactgcttc cttttgtcct ggttatcttt accctacagc gtcttcccat
     1321 gccacatcca ggccagatat ttcttttctg ttttgccatc tcgattcttc tgttacgact
     1381 ctgtaaagat gctggcaccg atgccacagc aacaatcgcc ctgttcagtg ttggtctcgt
     1441 tgagctcgct tggcatcagg tcaacttcag cgcagcgaac gctcttgacg cagcagtctg
     1501 gtacatcggc ttctggctcc ttttcctgct gtttccgttc ttctttcgcg accgtgtcca
     1561 gcagcgcgca cttccctgga tcaacgccgc attagccggc cctctgcaat tttcctttct
     1621 ctatctgtcg tatctcgaac tgaccggaaa aacactgccc ggaatcctgc ccgcgttgat
     1681 ggccttgccc tacctgtacg gattaaagtg tttgcatgcc tgcgatgggt tcccggtacc
     1741 agtaaagaat caacttctcg cctggtttgg cggaacctcg ctcttcttca tcaccctcat
     1801 ctttcccatt caattcgatc gagagtggct cacaatcggc tgggctctgc tgggagcgtc
     1861 actcgtgtgg ctgtatcagc gtgttccaca tgagggactc aaacattggg gcatcggtct
     1921 tctgctgatt tcattcgcgc gtcttgctga tcccgcggtt ttctcctact gtcctcggca
     1981 gccaactcca ttgatcaatt ggttcctcta cacgttcagt gtcacgggag ccagtctgat
     2041 taccggagcc accctgctcc gcccgccgca tcacgagtgg tttggcgtca acatccgcag
     2101 aattctgtat attcttggag cggtgctgtt cttcttttta ctgaacatcg agattgccga
     2161 cttctacagc actggcacga ccatcgtttt tcaattttct ggggaagccc tcgaacgcaa
     2221 tactgcttat tctgttggat ggagcatttt tgcctttatc cttctgcttg tgggtattgc
     2281 ctgcaattca cgcggcgccc gtcttggcag catcggtctt ctgaccgtaa cgattttcaa
     2341 agtcttcctc ctcgaccttt ggagtctcgg tggcctgtac cgggtcggct cactcgttgg
     2401 cctcgccgga gccctgatca tggtgtcctt tctctatcag aaattcgtca ccgttcgcgc
     2461 tcctgtttca cgagaacccg aatcaccgca cc
//
LOCUS       DAORHD010000742         2491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50694_length_2491_cov_3.927750, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000742 DAORHD010000000
VERSION     DAORHD010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50694_length_2491_cov_3.927750"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PKO06_14800"
     CDS             <1..565
                     /locus_tag="PKO06_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNV70968.1"
                     /translation="ILRNKATLSGNIVNASPIGDATIMLLALGARVVISATDGNKREQ
                     PLDTFYLGYKKTALACGDLVSEIIIPLLKKGERYHFEKVSNRTTLDIAAVNTALRLTT
                     APDGTIAGLRLSAGGVGPIPLLLSGIEVFHGRRPDEQFASELAQMAQNAVRPIDDIRG
                     SAEYKKLLLGQLIRAHFQQSVLAKEAV"
     gene            562..>2491
                     /locus_tag="PKO06_14805"
     CDS             562..>2491
                     /locus_tag="PKO06_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV70969.1"
                     /translation="MKNEDMLMHVRGTSRFVDDIPTSAGALHAVLFTSPVARGRIRSL
                     DISKAVTAPGVAAVYTHRDIPGLNQVGHVFKDHPLLAIDTVEYIGQPIALVVADTRRA
                     ARRAVKLIEVDIEKIVPVFNPREAAAKGMIHGKKRIMLSGDPDAAFGTCAHVIEGRLA
                     SGPQEHFYFETQRALAVPGEHDTIKVYASAQSPGAFHHHLAEVLGIPMHKVELDIRRL
                     GGGFGGKESTAVWIVAPALAAFLLKRPVKLVLERNEDIATTGKRHAYEFDYRLGLDAN
                     GQILAYDVTLFQHAGACADISLAVLGRSLLHVCGSYRVPNLRATALSCRTNLPPNNAF
                     RGFGVPQGTFAIEAALTRAAEVMGLNAEEIKRRNLLREGDSLPYGVQLSEVNATRCWE
                     TLAGKIDFTARRAAVTAYNQSHTDTKRGLAVVPVCFGISFAQTALNQASSLVNIYVDG
                     SVSVSTGAVEMGQGVNAKLRIIAARTLGIPLDCVRIDTTNTSRIPNASPTSASTGADL
                     NGMATKYACDRLRTRILEFAAKHLQLSPTDIEIRDGEVYGAGKPTGMSWAKLAAAAQW
                     ARVDLSEHGFYATPNLHYDVEKERGRPFAYYSYATSLTEVLLDCVRGTYTVESVDIVQ
                     DVGESLSAEIDRGQIEGAV"
BASE COUNT          497 a          775 c          714 g          505 t
ORIGIN      
        1 gattctgcgc aacaaagcca cgttgtcagg gaacatcgtc aacgcatctc cgatcggcga
       61 cgccacgatc atgctgcttg ccctgggtgc tcgggtcgtc atctccgcta cggatggcaa
      121 caagcgggaa cagcccctcg acaccttcta tcttggatac aaaaagaccg cgctggcctg
      181 cggtgacttg gtgagcgaga tcatcattcc cttgctgaaa aagggcgaac gctatcattt
      241 cgagaaggtt tccaatcgca ccacccttga catcgccgcg gtcaataccg cattgcgact
      301 gaccactgca cccgacggaa ccattgctgg cctgcgcctt tcggcaggtg gagtcggccc
      361 gataccactt ctgttgagtg gtatcgaggt ctttcacggt cgacgtccgg acgaacagtt
      421 cgcctcagaa ctcgcccaga tggcccagaa tgccgttcga cccatcgatg atattcgcgg
      481 cagtgcggag tataagaaac tgctcctggg acaattgatt cgcgcccatt ttcagcagtc
      541 ggtgttggcc aaggaggcgg tatgaaaaac gaagatatgt tgatgcatgt ccgaggcact
      601 tcgcggttcg tcgatgatat cccgacctct gccggcgctt tgcacgcggt tcttttcacc
      661 tcaccggttg cccgaggtcg cattcgcagt ctcgacatct cgaaagccgt caccgctccg
      721 ggcgtggcag cggtttacac ccatcgggac attccgggcc tcaatcaggt cggccatgtc
      781 ttcaaggacc accccttact ggcaatcgat accgtcgagt acattggtca gcccatcgcg
      841 ttggtcgttg ccgacacccg gcgcgccgcc cggcgagcgg tcaagctgat cgaggtcgac
      901 atcgagaaga tcgtaccagt attcaacccg cgtgaagcgg cagcgaaggg catgattcat
      961 gggaagaaac gcatcatgct cagtggtgac ccggacgcag ccttcggcac ctgtgcccat
     1021 gtgatcgagg gtcgcctggc aagcggcccg caggagcatt tctattttga gacccagcgt
     1081 gctctggccg ttcccggcga acacgatacg atcaaggtgt atgccagcgc ccagtcacca
     1141 ggcgcgtttc atcatcatct tgccgaggtg ttgggtatcc cgatgcacaa ggtggaactc
     1201 gacattcgcc gtctgggtgg cggtttcggc ggcaaggaat cgaccgccgt ctggatcgtc
     1261 gcccccgccc tggcggcatt tctgctcaaa cgcccggtca aactggtact cgagcgcaac
     1321 gaagacatcg ccaccacggg caaacggcac gcctacgaat tcgattaccg cctgggtctc
     1381 gatgccaacg ggcagattct ggcgtatgac gtgacgctct ttcagcatgc cggtgcctgc
     1441 gccgacatct ccctcgccgt tctgggccgc tctcttctgc atgtctgcgg ctcctatcgc
     1501 gtgccaaacc tgcgggcaac cgcactcagc tgtcgcacca atcttccacc caataatgcc
     1561 tttcggggtt tcggcgtgcc gcagggaacc tttgccatcg aagccgccct gacccgcgcg
     1621 gccgaggtca tggggctcaa tgctgaagaa atcaaacgac gcaaccttct gcgcgagggt
     1681 gactctcttc catacggtgt gcagctctcc gaggtcaatg cgactcgctg ctgggaaacc
     1741 ctggcaggca agatcgactt caccgctcgg cgtgccgccg tcacggccta caatcagtcg
     1801 catactgaca ccaagcgcgg gctagcggtc gttcccgtct gcttcggaat ctcgtttgcg
     1861 cagaccgcgc tcaaccaagc cagttcactg gtcaacatct acgtggacgg tagcgtttcc
     1921 gtcagcaccg gcgcggtcga gatgggacag ggggtcaacg ccaaacttcg catcattgcc
     1981 gcgcgcacac tgggaattcc tctggactgc gtgcggatcg ataccaccaa cacgtctcgc
     2041 atccccaatg cctcgcccac gtcagccagc accggggctg atctgaacgg tatggccacg
     2101 aagtatgcgt gtgaccggct tcgcacccgg attctggagt tcgccgcaaa gcatcttcag
     2161 ctgagcccga cagacatcga gattcgcgat ggcgaagttt acggcgcggg aaagcccacc
     2221 ggaatgagct gggccaagct tgcggcggcg gctcagtggg cccgggtcga cctttccgag
     2281 catggatttt atgcgacgcc caacctgcac tacgacgtgg agaaagagcg tggacgccca
     2341 tttgcctact acagctacgc gacttccctg acggaggtac tgctcgactg cgttcgcggc
     2401 acgtacaccg tggaatccgt ggatatcgtg caggatgtgg gggagtccct gagtgccgaa
     2461 atcgatcgtg gtcagattga gggagctgtc a
//
LOCUS       DAORHD010000743         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50771_length_2489_cov_3.432210, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000743 DAORHD010000000
VERSION     DAORHD010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50771_length_2489_cov_3.432210"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PKO06_14810"
     CDS             complement(<1..1434)
                     /locus_tag="PKO06_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70970.1"
                     /translation="MRRFIGRALLTASLAMLPFMTPAFAQEAAPEQPVAAADEHDEAW
                     VDGTHYFEEEARRLAETETRESLAEFDLRTKGIVQYADRKPLQVGDVLNFTIFNIASK
                     KNEQIAAVLKRIGKHCYIWVERGRTVSNAAIERTARTFDEKIYPTNRAYFGAEWNPGI
                     DGDPRIYLLFLDVRDGYNPSAGRGGFVAGYFNAGDCYPRAKSAGSNEKEIIYLDINPG
                     NPDKPNYLSVLAHEFQHMIHWYHDAKEFTWVNESMSQLASYLNGFNHPSQVLAFMQGG
                     DNNMLSWSSDNMLANYGQVYLFSYYVMKTFAQTVKEQATVTRYVVESDQNGSRGYLAA
                     LKKINENPQFKNIFRDFCVTNFLNDPNSAKGRYSYYPQLQKFLLRPMKHHNEPPFSGK
                     GKVCSWSARAVKMNFAPFEGKVRVKFQGQKQVGDRYTNEFDVAAVFLDSKRKTPPTVE
                     WLPVKNFAVDCILNNLAGRHDTLMLVVV"
     gene            complement(1708..>2489)
                     /locus_tag="PKO06_14815"
     CDS             complement(1708..>2489)
                     /locus_tag="PKO06_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70971.1"
                     /translation="FSVSLGVFLLQIVLLVAGVSPSYAFGMQMAYLGLFWLDVSFFWK
                     CSRPSIDKTLQAISNRKLLLSLNQKRMALELGKLEHAKKTAALAEVEKKLRKSHELVT
                     REIEILQIDENYLTSLRLFYRWKIIIYAVVKGLPRLNVGDLDAFEKLIDGLEADIQQC
                     SAVISAEVHRGRTAAREVYDIITQSLFLHDRIREALAARRAHQILSEVDLLSSMYDIP
                     DQATPIIIKDIDCTSEILNRVDAGYHKIQADAATNQTLREE"
BASE COUNT          508 a          667 c          758 g          556 t
ORIGIN      
        1 gacaacgacg agcatcagcg tgtcgtgacg acctgcgagg ttgttcaaaa tgcagtcgac
       61 cgcaaagttc ttcaccggta gccactcgac ggtcggtggg gtcttccgct tcgaatcgag
      121 gaacaccgcg gcgacgtcaa actcgttggt gtaccggtcg ccgacctgct tctggccctg
      181 aaactttact cgcaccttcc cttcgaatgg agcgaagttc atcttgacgg cccgggctga
      241 ccagctgcac acttttccct tgccggaaaa gggtggctcg ttgtggtgct tcattggccg
      301 cagcaggaac ttctgcagtt gcggatagta actgtatcga cctttggccg agttcggatc
      361 gttcaaaaag ttggtgacgc agaagtcgcg aaagatgttt ttaaactgcg gattctcgtt
      421 aatcttcttc agagcggcga gatacccgcg cgacccgttc tgatcgcttt cgaccacgta
      481 gcgggtgacg gtcgcctgct ccttcaccgt ctgggcgaag gttttcatga catagtagga
      541 aaacaggtag acctgtccat agttcgcgag catgttgtca gaactccagg agagcatgtt
      601 gttgtcgccc ccctgcatga aggcaaggac ctgagaagga tgattgaacc cgttcagata
      661 cgatgccagc tgagacatcg attcgttcac ccaggtgaac tccttggcgt cgtgatacca
      721 atggatcatg tgctggaact catgggctag aacgctgaga tagttcggct tgtccgggtt
      781 cccggggttg atgtcgaggt agatgatttc cttttcgttg cttccggcgc tcttggcgcg
      841 tgggtagcag tcaccggcat tgaaataccc ggcgacaaac ccgccccggc ccgccgaggg
      901 gttgtacccg tcgcgcacat cgagaaacag caggtagatg cgcgggtcgc catcgatgcc
      961 agggttccat tctgcgccga agtaggcgcg attggtcggg tagatcttct cgtcgaaagt
     1021 gcgcgccgtt cgctcgatcg ccgcattcga aaccgtgcga ccgcgttcca cccagatgta
     1081 gcaatgcttg ccgatgcgct tcagaaccgc ggcaatctgc tcgttcttct tcgaggcgat
     1141 gttgaaaatc gtgaaattca ggacgtcgcc gacctgcagc ggcttgcgat cggcatactg
     1201 cacgatgcct ttggttcgca ggtcaaactc cgccaagctt tcgcgggtct cggtctcggc
     1261 cagacgtcgg gcttcttctt cgaaatagtg cgtgccatcg acccaggctt cgtcgtgctc
     1321 gtcagcggcg gctacgggct gttcaggagc ggcttcctga gcgaatgccg gcgtcatgaa
     1381 cggcagcatc gccagagatg cggtgagtaa cgcccgaccg ataaacctgc gcatagtgtg
     1441 ctcctgcggc caggatgtaa agcctatgcg gtaagtatac caaatccccc cctgtacccg
     1501 gaagaggtgg acacggggtg catctcacag tcagaatttt gtctgaggtt ccagagagat
     1561 ccaacgcagg caatcaagct gaatacagcg agaagggggg acccagtatt gttcagcagt
     1621 ctgctggttc tggggcttga aactggggaa ctggccgagg ttttccacag cgcttgcaca
     1681 cgttttgtac gctgcgttct cttgacgcta ctcctcgcgg agggtctggt tggtggcggc
     1741 gtcggcctgg atcttgtggt agccggcgtc gacgcggttg aggatctcgg aggtgcagtc
     1801 gatatccttg atgatgatcg gggtcgcctg gtcaggaatg tcgtacatgg aggaaagcag
     1861 gtcgacctcg gaaaggatct gatgggcgcg tcgtgcggcg agggcttcac ggatgcggtc
     1921 gtgcagaaac agagactgcg tgatgatgtc atagacctcg cgcgcggcgg tgcgaccgcg
     1981 atgaacctct gcgctgatga cggcggaaca ctgctgaatg tccgcttcga gaccgtcgat
     2041 gagtttttcg aacgcgtcga ggtcgccgac gttgaggcgc ggcagcccct tgaccacggc
     2101 atagatgatg atcttccagc ggtagaacag acgcaggctg gtgagatagt tctcgtcgat
     2161 ctgcagaatc tcgatctctc gggtgaccag ttcgtgactc ttgcgcagct tcttttcgac
     2221 ttcggcgagg gccgcggttt tcttggcgtg ttcgagtttt ccgagctcga gcgccatgcg
     2281 cttctgattc agcgacagca acagtttgcg gttactgatc gcctgcagcg tcttgtcgat
     2341 tgatgggcga gagcacttcc agaaaaagga aacgtcaagc cagaacaagc ccagataggc
     2401 catctgcatc ccgaacgcat aggacggaga cacaccggcg accaagagca cgatttgcag
     2461 cagaaaaacg ccgaggctga cggaaaaag
//
LOCUS       DAORHD010000744         2489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50779_length_2489_cov_2.856615, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000744 DAORHD010000000
VERSION     DAORHD010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50779_length_2489_cov_2.856615"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKO06_14820"
     CDS             complement(<1..574)
                     /locus_tag="PKO06_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70972.1"
                     /translation="MANARYLDLEECRCCGYVVTEKETVCPGCGVRVPRASDVWLVPA
                     ALLKVPIVRDPAAKHPLLKRSWASLDDVYEVPQEEPLPRMVRDKLNEMLAQGRREIAD
                     AAPSIKILFYCFIGAVVCGVNVQGMMFLGALVIIGVLFFKLVVAMMNAAGPLSGILVT
                     GMKALQAAFFARRVPGSVMEKEQKLHNRLRH"
     gene            complement(552..1889)
                     /locus_tag="PKO06_14825"
     CDS             complement(552..1889)
                     /locus_tag="PKO06_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70973.1"
                     /translation="MLHSDRVFDVMNSHILAGLIRSACHEIILFTPGIDVEIAQALIE
                     SQTRLAREKFHVIVDASEKSCRFGYGTIEGLGLLMQQRIPLRNAPGLRISGVFVDGRG
                     WVFSPTPLLIEAGKENDRQPNALRVSKEQFHLLKQATLGESQIRKEAGLPDGLVHVST
                     DQEALRAAAPEIGKGDVTTEVTEAIAVNLKNNPPQKFDLARKVNVYNTQIEYVEIALN
                     GCRFQAKKVKLPSEFLIGDADEYLNRNLQSIFNLTGKDSDLVPRRFTEKFEQFRKQYL
                     KPIPKLGQVILKCRKTEFMAGVEQFRRDLEQIKEELKTRWDKGMNKTCEKLIEILMPR
                     VKKNPPLSLRNQIVENEVSDKVAHAYILDGLAKVFPTYEKITAGMELTCTFKAITYET
                     LNDAGFQTEIERQFPFIGWDKLFSEYDAVRGEDLCPSPKQRSLEDANGQRKIP"
BASE COUNT          556 a          689 c          603 g          641 t
ORIGIN      
        1 agtgccgcaa tcgattgtgc agtttctgtt ctttttccat gaccgaacca ggcacacgac
       61 gagcgaagaa cgccgcctgc agcgctttca ttccagtgac cagaatgcct gagagcggac
      121 cagccgcatt catcatggca accaccagtt taaagaacaa cacaccgatg atcaccagag
      181 ctcccaagaa catcattcct tgtacattta ccccacacac caccgcgcca atgaagcaat
      241 aaaacaatat cttgatcgaa ggcgcggcat ccgcgatttc tcgacgtccc tgcgcaagca
      301 tctcattcag cttatcgcga accatgcggg gcaacggttc ctcttgcgga acttcgtaca
      361 cgtcatccag gcttgcccag cttcgcttca acagaggatg tttcgccgca ggatcacgta
      421 ctataggcac cttcaaaaga gcggcgggta cgagccacac atcagaggcg cgcggcaccc
      481 gcactccaca gccgggacac accgtttctt tttccgtcac cacatatccg caacaacggc
      541 actcctcaag gtcaaggtat cttgcgttgg ccatttgcgt cctccagcga acgttgcttg
      601 ggtgatgggc agaggtcttc gccgcgcacc gcgtcgtatt cagagaagag tttgtcccag
      661 ccgatgaacg ggaactgccg ctcgatctcg gtctgaaaac cggcatcatt gagagtctcg
      721 taggtgatcg ccttgaacgt gcaggtgagc tccatcccgg cggtaatctt ctcataggtt
      781 ggaaacactt ttgccagtcc gtccaggatg tacgcatgtg caactttgtc gctaacctca
      841 ttttcgacga tctgatttct cagacttagc ggagggttct tcttgacacg tggcatgagt
      901 atctcgatca gcttctcaca cgtcttattc atgcctttat cccagcgggt tttcagttct
      961 tccttgatct gttcgaggtc tcttcggaac tgctctactc ccgccatgaa ctcggttttt
     1021 cggcatttca gaataacctg gccgagtttg ggaatcggtt tcagatactg ctttctgaat
     1081 tgttcaaatt tttccgtgaa ccggcgagga acaaggtccg agtccttgcc ggtcagattg
     1141 aagatggatt gcaggtttcg attgagatac tcatcggcat cgccaatgag aaactcggaa
     1201 gggagcttga ctttctttgc ctgaaacctg cagccgttca aggctatttc gacatactca
     1261 atctgggtgt tgtaaacatt caccttccgt gccagatcaa acttctgggg cggattgttc
     1321 ttcaggttca ccgcaatcgc ctcggtcact tcggtggtca cgtcaccttt gcctatttcg
     1381 ggggcggccg ctctgagagc ctcttggtcg gtcgatacat ggaccaaacc atcgggcagc
     1441 ccggcctctt tgcgtatctg cgactcgccg agggtcgcct gtttcagcag gtgaaactgt
     1501 tctttggata cgcgaagggc attgggctgc ctgtcgtttt ctttcccggc ttctatcaac
     1561 agcggggtcg gagaaaacac ccatcccctg ccatcgacaa acacaccgga aatgcgcagg
     1621 ccaggagcat tgcgcaacgg gattctctgt tgcatcagaa gaccgagacc ttcgatagtg
     1681 ccgtatccga aacgacagct cttctctgac gcatcgacga tgacatgaaa tttctccctg
     1741 gccagacgag tctgcgattc gataagcgcc tgcgcgatct cgacatcgat gcccggagtg
     1801 aagaggatga tttcgtgaca ggcactgcga atcaacccgg cgagaatgtg cgagttcatc
     1861 acgtcgaaaa cccggtctga atgaagcatt tgaaggctcc ctttgattgt ccttgtctgg
     1921 aaacagacga caaacgaaaa agcagatcag gcacgccgac cgtcagacac ctgtcaacct
     1981 ctcacacttc gaatatattt cgacactgtc gaattgtcaa tataatattc ctaaaactaa
     2041 tttttctgtt tttagataag gtttttgctg gctatttaag tgtcatgttg tgaattttca
     2101 attttctgaa cgatcgtttc cagcctggtt cacgaagctc ccttatcgtt cttcgttatc
     2161 aaactctgca aggctctctt ctgaaaggca ataatcgtcg cgctgaaaga cttcttcctg
     2221 gtctcgttca gtccgagata ctccgcgact ttcgccacag ctggcgatgg tttccattct
     2281 cggttcaacg ttccgagaat ctcggctttc gctttccgat cgcgcggcga tgtttcgcag
     2341 acggtcgtgg ggctggtcag cctggcacgc aaagtgggaa cgacctgagg gtgcagaagc
     2401 tccttgccaa tttatgtgac cccgcccagc atctgttgca tcttctgttg gaactgatct
     2461 gattcaacgt gcagctggtc aatgaaccg
//
LOCUS       DAORHD010000745         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50797_length_2488_cov_3.838471, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000745 DAORHD010000000
VERSION     DAORHD010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50797_length_2488_cov_3.838471"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKO06_14830"
     CDS             complement(<1..329)
                     /locus_tag="PKO06_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70974.1"
                     /translation="MRPLKLLCIVGYLLFMGLAFDNTIVDALVFYVSDQDGAPVMKSG
                     KIKVRPLARSGDTEAADEAVAAVQFQSRPVNQMQQAAGTQPALAMQMAQPREAQNTIE
                     MKPVPSV"
     gene            complement(372..884)
                     /locus_tag="PKO06_14835"
     CDS             complement(372..884)
                     /locus_tag="PKO06_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV70975.1"
                     /translation="MVSAKKRETKEKNRGMFLFFLSGMFLLQIVFAFTIHGDIGPQAA
                     LNTSCQAPASPLLMGRIDTILSAPNQTETARMHPMPISEAPAPVVKPREVLAREQATL
                     HQPTSPVNQGRFAEYTVQRGDTLERISRKLCGTTSLVSALVRLNRLSNERGLQCGARL
                     RVPRTLMAKK"
     gene            1195..>2488
                     /locus_tag="PKO06_14840"
     CDS             1195..>2488
                     /locus_tag="PKO06_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV70976.1"
                     /translation="MNVLLLNPPGRLFYTRDYFCSKVTKVEYAEHPVDLLMLSGIIGA
                     HHQVTLLDAMARRLSVEVTLGEIERVRPEAIIFLAGSASWKEDFPFMEEVRRRWPHLR
                     LIGLGDLFLNRRVFDAVPWLDAVLHDFTTDDVLRVLEGKASQARNLFYRSGTEVQAPG
                     PGEKRGSFEVPVPRHELFLDLPYTFPFARYHPFATLLTDFGCPFACRFCLYSTLGFRQ
                     RPVSNVVAELERLRVLGVRELFVKDQAFGADRARAVALCDEMVARGPFSWTCFMRATS
                     VDADLLRRLRQAGCHTIMFGVESAGEEILTRVDKRVTVLQVQEAFRLCRQGGMRTVGI
                     FILGFPGEDEASCRRTIQLAVDLDCDFASFNVFVPKHSTPMRAELEQAGRVSDDPLET
                     MDQSGIAASSASDCLSAADLDRLRTEANRTFYLRPSYVL"
BASE COUNT          475 a          678 c          748 g          587 t
ORIGIN      
        1 tgtacggaag gaactggttt catctcaatt gtgttctgcg cttcgcgtgg ctgggccatc
       61 tgcatggcca gagccggctg cgttccggcc gcctgttgca tctggttcac cggccgtgac
      121 tgaaactgca ccgcggcgac agcttcgtcc gccgcttccg tgtcgccgct gcgggcgagt
      181 gggcgaacct tgatcttccc ggacttcatg acaggcgcgc cgtcctgatc cgaaacgtag
      241 aatacaaggg cgtcgacaat cgtgttgtca aaggcgagcc ccatgaacaa cagatatccg
      301 acgatacaca acagtttaag aggtctcata aggccgtcct gatgctcccg tgtggtgtgt
      361 tcctgttgct gttacttctt ggccatcagt gttcgaggaa cacgaaggcg agcgccgcac
      421 tgcagaccac gctcattgga cagacgattg aggcggacaa gagctgagac caggctggtg
      481 gtaccgcaca gcttgcgcga aattcgttcc agcgtgtcgc cgcgctgaac cgtgtattca
      541 gcgaatcgtc cctgattgac gggagacgtg ggctggtgca gcgtcgcctg ctcgcgtgca
      601 agaacctcgc gcggtttcac gacgggcgca ggagcttcgc tgatcggcat ggggtgcatg
      661 cgtgcggttt ccgtctgatt cggggccgag agaatcgtgt cgatgcgccc catcagcagc
      721 ggcgaggctg gcgcttggca cgaggtgttc aaagccgcct gaggcccgat atcgccatgg
      781 atcgtaaagg caaagacgat ctgcagcagg aacattcctg agaggaagaa cagaaacatt
      841 ccccgattct tttccttcgt ttcgcgcttt ttggcggata ccatgacgaa aacctccttg
      901 caggctagtc aacttatcgg cagcgtctcg gcctaacctt agaaatgagg cagattggcg
      961 aaaccggaaa atgaatcggc ggtgtgggag catggaaaac ctgcatcaaa agccgcaagg
     1021 acctctgcgt ctctggaaaa tcgtgacaga atgcgctacc atacgtccag attgtgaagc
     1081 gcgctgtcgt gaacagcctg cccttcccgg agccagagtc atgcttacac agggaaacaa
     1141 actcaggata ttcttctgta gccaattttg gcgtcacagt cctatcggac gagcatgaac
     1201 gttctcctcc tgaatccgcc cgggcgtttg ttctacacgc gcgactactt ctgcagcaaa
     1261 gttaccaagg tcgagtatgc ggaacatccg gtcgacctgt tgatgctcag cggcataatc
     1321 ggagcgcatc atcaggtgac tctgctcgat gccatggctc gtcgactttc tgtggaagtg
     1381 accctggggg agattgaacg tgtacgtccc gaagccatca tttttctggc tgggagtgcg
     1441 tcgtggaaag aggactttcc attcatggaa gaagtgcgcc gacgatggcc gcacctcagg
     1501 ttgatcggtc tcggggatct gtttctcaat cgtcgcgtgt ttgacgctgt tccgtggctt
     1561 gatgccgtgc tgcacgattt cacgaccgac gatgtgctgc gggttctcga gggaaaggcg
     1621 agtcaggctc gcaatttgtt ctatcgatcg ggaaccgaag ttcaggcccc tggaccgggg
     1681 gagaaaagag gttcctttga ggttcctgtg cctcgtcacg aactctttct tgatcttccc
     1741 tatacgtttc cgtttgcccg gtatcatcct tttgccacgc tgctgaccga cttcgggtgc
     1801 ccctttgcct gtcgcttctg tttgtattca accctcggtt ttcgccagcg ccccgtgtcg
     1861 aacgtggttg ccgaacttga gcggttacgg gttctcggag tacgtgagct gtttgtcaaa
     1921 gatcaggcgt ttggggccga tcgtgcgcgg gctgttgcac tctgtgacga gatggtcgcc
     1981 cgcggccctt tttcctggac ctgcttcatg cgggccacgt cggtcgatgc ggatctgctg
     2041 cgtcgtttgc gccaggcggg atgtcacacc atcatgttcg gggtcgagag tgcgggcgaa
     2101 gagattctca cgcgggtcga caagcgagtt acggtgctgc aggtgcagga agcattccgc
     2161 ctctgtcgtc agggaggaat gcgaaccgtg gggatcttca ttcttgggtt tccgggggaa
     2221 gacgaggcca gttgtcggcg aaccattcag ctcgcggtcg atctggactg tgactttgct
     2281 tcgttcaacg tgtttgttcc caagcattcc acgcccatgc gggccgaact ggaacaagcg
     2341 ggcagggtgt cggacgaccc tctcgaaacg atggatcaat cgggcattgc cgcatcgtcc
     2401 gcctcggact gtctctcggc ggctgatctt gatcgtctgc gtaccgaggc gaatcggaca
     2461 ttttacctgc gcccatcgta tgtgttga
//
LOCUS       DAORHD010000746         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50805_length_2488_cov_3.207152, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000746 DAORHD010000000
VERSION     DAORHD010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50805_length_2488_cov_3.207152"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKO06_14845"
     CDS             <1..466
                     /locus_tag="PKO06_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70977.1"
                     /translation="VSRRKGSEWEAVWTDEKNENPFYIGHAGVESLQVVQDIDVDGKV
                     EILSACAQSDVSPTLFRLFRWDGSKFDLVKKALITADKEDPTEFVWTDSKGDNSQVKC
                     WILYFDETESGSGLKAFVTSTFNDKYKSGQALVRHSPKGFVVEKWIEPLKAQ"
     gene            complement(571..1023)
                     /locus_tag="PKO06_14850"
     CDS             complement(571..1023)
                     /locus_tag="PKO06_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV70978.1"
                     /translation="MKVLLKRILLLVVLACGICTGCWMTGSIHSAAKEGKVDVVKRWL
                     ALGGDLNEKDPRGATPLHCAIEGGHRDVIKLLLQKGADVHQINDIGNTPLHEACWRGQ
                     FDTVQALVATGANPLAKAVDGKTPTDLARAADHQEIVNYLLALPARKP"
     gene            complement(1516..>2488)
                     /gene="hydF"
                     /locus_tag="PKO06_14855"
     CDS             complement(1516..>2488)
                     /gene="hydF"
                     /locus_tag="PKO06_14855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HNV70979.1"
                     /translation="AARQVLRKTDLALVVLEPLPHLDPTEIELLELLSSGKTPFLVIV
                     NKCDTGKPPESLLAELKRYTVVPVAVSARERSGVDDLRAVHMQSKLKDFDTGTIVGDL
                     VKPGDVVVLVVPIDLAAPKGRLILPQVQTIRDLLDADCIAITAKERELRAVIDGLRQP
                     PALVITDSQAFLKVAADVPRGVPFTSFSVLFARYKGDLAAYVEGIRQFIGRPVKKKIL
                     ISEVCTHHQMADDIGRVKIPRWLRQTYGGELQFEFCQGCSYPKNLAEFDLVVQCGGCM
                     VNRRAILDRIEDCRRQGVPITNYGILIAHLHGVLEDAIKPFGLSVHA"
BASE COUNT          539 a          782 c          662 g          505 t
ORIGIN      
        1 ggtgagccgc cgaaagggat cagagtggga agccgtctgg acggatgaaa agaatgaaaa
       61 ccccttctac atcggacacg ctggcgtcga gagtctgcag gtcgtgcagg atatcgatgt
      121 cgacgggaag gtcgaaattc tatcggcgtg cgcccagagc gatgtcagcc ccaccctgtt
      181 ccgtctgttt cgctgggacg gcagcaagtt cgatctggtg aaaaaagccc tgatcaccgc
      241 ggacaaggaa gaccccaccg aattcgtctg gaccgactcg aagggcgaca actcccaggt
      301 caaatgctgg attctttact ttgacgaaac cgaatcagga agcggactca aagcgttcgt
      361 caccagtaca ttcaacgata agtacaagtc aggccaggcc cttgtgcgcc attcaccaaa
      421 gggattcgtc gtcgaaaagt ggatcgaacc cctgaaggcc cagtagcagc ggtaacgatc
      481 gtccacgcag cagcaaaccc ccggctcctt gtcgggggct tgccattttg tccgatgtcg
      541 aacgcgcgcc ggtgttcacc aaaacttgtg tcacggcttt cgggccggaa gtgccagcag
      601 atagttgacg atctcctgat gatcagccgc ccgcgccaga tccgtggggg ttttgccatc
      661 gaccgctttg gccagcgggt tcgctccggt ggctacgagc gcctgcaccg tatcgaactg
      721 cccgcgccag caggcttcgt gcaggggagt gttgccaata tcattgattt ggtgcacatc
      781 ggcacccttc tgcagcagca gtttgatcac gtcgcgatgg cccccttcga tcgcacaatg
      841 taacggggtc gccccgcgcg gatccttctc gttgagatca cctccgagag ccagccaccg
      901 tttcaccaca tcgactttcc cttctttggc cgcactgtga atcgatcccg tcatccagca
      961 gcccgtacaa atgccgcacg ccaacaccac cagtaacaaa atccgtttca ataggacttt
     1021 cattgtatct cccgttcgcg tggctgtcgg gcgatgcccg actcatcagg ctcacggttg
     1081 caatcaggcg gaggtccttc catcgcacct gatctcacgt gcctgctcgt ctctgagctc
     1141 aaagaaactc gagcactggg tccgttcgcc gtcgcctccg tcccgttcgc ggcagtcacc
     1201 ttcacctcgc ttgcaatcgg ctcagtcgcg gtcgcctcat tcgcattctc ctcaatcgca
     1261 cgcctttcca gacgacgctg tctctttcgc tgccgactgt tcagcttttt tcgcgccgag
     1321 cgccggtccg aggcatccag taacgcgtga acgacgagaa caacgatccc gctcacggcg
     1381 tagagcagca ggttgagttg ctttgcgctc caactgccgg acaccggcaa ccaatctgca
     1441 tttccgataa tggtaggggc gatcaggagg acggcaacgc aaaccatgcc gttgaccagc
     1501 ttgaacccgg aaggctcacg catgcacgga aagaccgaag ggcttgatcg catcctcgag
     1561 tacgccatgc agatgggcga tgagaatacc gtaattcgtg atcggcaccc cctgacgccg
     1621 gcagtcttcg atccgatcga gaatggcccg acgattcacc atacagccac cgcactgcac
     1681 cacgagatca aactcagcca ggtttttggg gtaactgcac ccctgacaga attcgaactg
     1741 gagttcgcca ccatacgtct gtctcagcca ccgaggaatc ttgactcggc caatatcatc
     1801 ggccatctga tgatgtgtac agacttccga gatcagaatc ttctttttca cggggcgccc
     1861 gataaactgt cgaatgccct ccacgtaggc ggcgagatcg cccttgtagc gggcgaacaa
     1921 cacggagaaa ctcgtaaatg gcaccccccg gggaacatct gctgccacct tcagaaaggc
     1981 ctgcgaatcg gtgatcacca gcgccggcgg ttgccgcaga ccatcaatga cggcgcgcag
     2041 ttcccgttcc ttcgccgtaa tcgcgatgca atcagcatcg agcagatcac gaatcgtctg
     2101 cacctgcggc agaatcagcc tccctttcgg cgcagccaga tcgatcggta ccaccagtac
     2161 gacaacatcg cccggtttca ccagatcacc cacgatcgtc ccagtgtcaa aatctttgag
     2221 cttgctctgc atgtgtaccg cacgcagatc atcgaccccc gaacgctcgc gggcactgac
     2281 ggcaaccgga acgacagtgt accgtttgag ttccgccagc agcgactccg gcggctttcc
     2341 ggtgtcgcac ttgttgacga tgaccagaaa tggggtcttg cccgagctga gcagttccag
     2401 caactcgatc tcggtcgggt cgagatgcgg caacggctcg agtacaacca gtgccagatc
     2461 tgtttttcgc agcacctgac gcgccgct
//
LOCUS       DAORHD010000747         2486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_50880_length_2486_cov_3.326203, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000747 DAORHD010000000
VERSION     DAORHD010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50880_length_2486_cov_3.326203"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PKO06_14860"
     CDS             <1..648
                     /locus_tag="PKO06_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70980.1"
                     /translation="WSQYQEILYDKDMNLPYLESCAKQFKREDPKKAGSYQYVDLTDP
                     SVTAKDALCVEAVNAVLMYRLATTLPDGANKVNTAFYKGNYPWRSMEGFLKGFCKLYP
                     SDAQNVSEILDQMTGNKMSEAEIRKLCGNVTLNRPATIAVSESSPSAPIEHVSSPTPT
                     AAAAPTSSEGLALKAEFEKTYLALVKAAQEGKEDVMRALQEKMQNLRRKMNAIAR"
     gene            719..1567
                     /locus_tag="PKO06_14865"
     CDS             719..1567
                     /locus_tag="PKO06_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70981.1"
                     /translation="MPELLREMLTQGHARPLHSDQIRDTFDTEEDQAEYELNYRPPYR
                     DTRFFKTHPAGYNVVRSLGAVFALGGWVATVFVAALFSELKGFAGVGAVIAGLIVGLT
                     LGFTLPEILWRGVGSTIRSALRPLGWFGGLPMIFLCLCLFAVFAGVLRLVIPQPSDSS
                     ATPSSQSTPLPKPAPVVWKGSDWQILLDANGKPREMPRQDALTACSALGPGWRLPLAS
                     DTAWVLQRLKTMHGGILFHADGPLVPVPGTKLIEKPFLMYRWPDGWIIDYTRAESTMK
                     VLCIRP"
     gene            1646..1867
                     /locus_tag="PKO06_14870"
     CDS             1646..1867
                     /locus_tag="PKO06_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70982.1"
                     /translation="MSRGTEVILYMSGVFLLLFLVCAVAAGILSFFVLDKETSAGKRI
                     GFIVGGAAIIAVVLVILVWIRAGGVSAFI"
     gene            2006..>2486
                     /locus_tag="PKO06_14875"
     CDS             2006..>2486
                     /locus_tag="PKO06_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70983.1"
                     /translation="MRLRLGSLLTVVVLSVLSLILGCGKERGSVEPPPPVAGSGEALY
                     RIKSPDKTVIALERGKKRAEVPVPPEYKIYDQDLIENKGDKPAVMHDLQKRHTFNLAP
                     GARLKIGSQAITMFFGSTTYEFENIGGEFRIVLPKAALVIKGTKFQVDVTENGSAKVQ
                     "
BASE COUNT          525 a          726 c          696 g          539 t
ORIGIN      
        1 tggtcgcagt atcaggaaat tctgtacgac aaggatatga acctgcccta tctcgaaagc
       61 tgcgccaaac agttcaagcg cgaagatccg aagaaagcag gatcgtacca gtacgttgat
      121 ctcaccgacc cctcggtgac cgcaaaagac gccctgtgtg tcgaagcggt taatgcagtt
      181 ctgatgtacc gtctggccac cacgctcccg gatggcgcga acaaggtcaa cacggcgttc
      241 tacaaaggga actatccgtg gcgctcgatg gaagggttcc tgaaagggtt ctgcaaactg
      301 tacccgtcag atgctcagaa cgtatcagag attctcgatc agatgacggg aaacaagatg
      361 agcgaggccg agatacgcaa gctctgcggc aacgtcacgc tcaatcgccc agccaccatc
      421 gccgtttctg agtcgtcccc gtctgcgccc atcgaacacg tctcctcccc caccccgact
      481 gctgccgcgg ccccgacctc cagcgaggga ctggccctga aagccgagtt tgagaagacc
      541 tatctggccc tcgtgaaagc cgcacaggaa ggcaaggaag acgtgatgcg cgctctgcag
      601 gagaagatgc agaacctgcg ccggaaaatg aacgcgatcg cccgctgacg gacgtcgttt
      661 gaactccagc gacaggccga accacggctc gcccccgaca tccgagcgcg gtaacgccat
      721 gccggaactg ctccgcgaga tgctcacgca ggggcatgca agacccctgc attcagacca
      781 gattcgtgac acattcgaca cggaagaaga tcaggccgag tatgaattga attatcggcc
      841 tccctatcgc gacacccgct tcttcaagac ccatcccgcc gggtacaatg tcgtcaggtc
      901 actcggcgct gtcttcgctc tcggcggttg ggtagcaacc gtattcgttg cggcattgtt
      961 cagtgaactg aaaggatttg ccggcgtggg cgcggtcatc gccgggttga tcgtcggact
     1021 gactctcggt ttcacccttc cggagatcct ctggcgaggc gtcggcagca cgattcgcag
     1081 cgctctgcgc ccgttgggct ggtttggcgg tctgcccatg atttttctct gtctctgcct
     1141 gtttgcggtg tttgccgggg tcttacgcct ggtgattccg cagccgtcag attcttcggc
     1201 aaccccatcc tcgcaatcaa caccgctccc caagcctgcc ccggtagtgt ggaagggcag
     1261 cgactggcag attcttcttg atgcgaatgg caaaccgcga gagatgccac gccaggacgc
     1321 tctcacggcc tgctctgccc tgggacccgg ctggcgtctg cccctcgcct cggataccgc
     1381 gtgggttctg caaagactga agacgatgca cgggggcatt ctctttcacg ccgatggccc
     1441 actcgtccct gtccccggca caaaactgat tgagaaaccc tttctgatgt accgatggcc
     1501 ggacggctgg atcatcgatt acacccgggc tgaatccaca atgaaggttc tctgcattcg
     1561 accgtaagcg aatgattggg tagcttcgtg agatccatgg tgctacactg ccagcagcat
     1621 aatgacactg gcaaaggagc actttatgtc cagaggaacg gaagtcattc tctacatgag
     1681 cggggttttc ctgcttttat tcctggtctg cgcggttgca gccggtattc tgtcgttttt
     1741 cgttctggac aaagagacgt cagcgggaaa acgcattggc ttcatcgtcg gcggtgcagc
     1801 cataatcgcc gttgtgcttg ttattcttgt ctggatccga gctggagggg ttagcgcgtt
     1861 tatctaactc gtacagacct ttgtctccat cccccactcg ctcgattgcc ggctttccct
     1921 gaagcttgcc agaaactgtt tttgtttctt cgcatcttgt ggcagaatga agacaggtga
     1981 cgtagtctgt gggaggaacc ggaatatgcg gttgcgcctg gggtctttac tgacggttgt
     2041 cgtcttgtcg gttctctcgt tgatcctcgg atgcggaaaa gagcgtgggt cggtcgaacc
     2101 cccaccgccg gtcgccggct caggtgaagc attgtaccgc atcaagtcgc ccgataagac
     2161 ggtcatcgcc ctggaacgcg gcaaaaaacg tgctgaggtg ccggtgccgc cggaatacaa
     2221 gatctacgac caggatctga tcgaaaacaa gggtgacaag ccggccgtca tgcatgatct
     2281 gcagaagcgc cacaccttta acctcgctcc tggagccaga ctgaaaatcg gctcgcaggc
     2341 gatcaccatg tttttcggtt cgaccaccta cgagttcgag aacatcggtg gtgagttccg
     2401 catcgttctt cccaaggccg ctctggtcat caaaggcacg aagtttcagg tcgatgtcac
     2461 cgaaaacgga agcgcgaagg ttcaac
//
LOCUS       DAORHD010000748         2482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51014_length_2482_cov_3.535641, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000748 DAORHD010000000
VERSION     DAORHD010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51014_length_2482_cov_3.535641"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1114)
                     /locus_tag="PKO06_14880"
     CDS             complement(149..1114)
                     /locus_tag="PKO06_14880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNV70984.1"
                     /translation="MKRLSVLMTLLASVAVMLIISLCGCGKPKGLTVKELRIATGSRS
                     GTYFPVGETIARALKEHFSDVHVQVLETDGSVQNMQLLQEGKANLALVQSDIANYAVQ
                     GKNMFIRQKILGVSGIAPLFPEVIQIITRRADAIKDLKSLKGKKVAVGTQESGTYYNA
                     QQILAQSGMWDEVNRVNLSPWEAMSSLERRSVDAFFFTTALPNPAISELSSRHEIAPI
                     ALPSGMIFNLTNTYPYYFKSQIPPGSYIGATEGVPTLEIHALLVSHQSISEDDLQLIT
                     RTLLGATNLDKLKKMHPQLAGLTRDSIRRPMSIPLAEGARKALAE"
     gene            1056..1805
                     /locus_tag="PKO06_14885"
     CDS             1056..1805
                     /locus_tag="PKO06_14885"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HNV70985.1"
                     /translation="MINITATLASSVIRTDSRFKLRSCFTRLGRESPLRKPTCIIGKI
                     GQNGKVTHDTFSSGRAESQDSGSSLIPNAPPRRLRVISECGVLIGLALALAQLRLFQL
                     PSGGSISIGSLPLLLVAARHGAFWGSLAGAIAGLLSAAAHPYIVHPIQFLLDYPLAFG
                     SLGLAGCWRWQPPWRAIAGVTLAGAVKLHCHVAAGVVFFSAGLPPGAAAWAGSYLYNL
                     SHMLPETVLCATIGWILAARHPHLLIPHTRE"
     gene            complement(1991..2182)
                     /gene="rpmB"
                     /locus_tag="PKO06_14890"
     CDS             complement(1991..2182)
                     /gene="rpmB"
                     /locus_tag="PKO06_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNV70986.1"
                     /translation="MARTCCVCNRGPKVGYQLSHSHRRSKRRWNINLQRKQVMVAGKL
                     TKGYVCTRCMGSEKVTHIV"
BASE COUNT          490 a          757 c          697 g          538 t
ORIGIN      
        1 aaccagcgag acttcgtgtt caccggatcg gccgccgaaa acgaccccta cgcgcacctt
       61 cgtgccggca accatgcgag acctccttcg cccggaaatg ggcttccatc accactatcg
      121 gcactccaag cggcaaacct gaatcgggtt attccgcgag cgctttgcgg gctccttcag
      181 ccagaggaat cgacatcggg cggcgaatcg aatcgcgcgt cagccccgca agctggggat
      241 gcatcttctt gagtttgtcg agattcgtgg cccccagcag cgtccgggtg atcagctgca
      301 gatcgtcttc ggaaatgctt tgatggctga ccagcaacgc gtgaatctcg agcgtcggca
      361 cgccctcggt cgcgccgata tagctgcccg gaggaatctg tgatttgaag tagtatggat
      421 acgtattggt caggttgaag atcatgcccg acggcagcgc aatgggcgcg atctcatgtc
      481 gactggacaa ctcagaaatc gccgggttcg gcaacgccgt cgtgaagaag aaggcatcga
      541 cgctgcgccg ctcaaggctg ctcatcgctt cccacgggct gagattcacc cgattcactt
      601 cgtcccacat tccggattgt gccaggatct gctgcgcgtt gtaataggtc ccgctttcct
      661 gagtgccgac cgcgaccttt ttccctttca ggctcttcag atctttgatc gcatctgccc
      721 gcctcgtgat gatctgaatg acttcgggaa acagcggcgc aatgccactg acgccgagta
      781 tcttctggcg aatgaacatg ttcttgccct gtacagcata attggcgata tcactctgta
      841 ccagagccag gttcgccttg ccttcctgca gcagttgcat gttctgaacc gagccgtcgg
      901 tctcgagcac ctgcacatgc acatcggaaa agtgttcttt cagtgcgcgg gcaatggtct
      961 cacccaccgg gaaataggtg ccagagcgag aacccgtggc aatgcgcagc tctttcaccg
     1021 tgagcccctt gggcttgccg cagccgcaga gactgatgat caacatcacc gcaacgctgg
     1081 ccagcagcgt catcaggaca gatagtcgtt tcaagttgcg atcctgcttc actcgtctgg
     1141 gtcgagagtc acctctccga aaacctactt gcattatcgg aaaaatcggg cagaatggca
     1201 aggtgactca cgatactttt tcatcgggca gagcggaaag tcaagattcg ggttcgtcac
     1261 tgataccgaa cgcccctccc cgccggttac gggtcatttc tgaatgcggg gtcttgatcg
     1321 ggctggcgct ggctttggca caacttcgcc tgtttcaact gccctcgggg gggtcgatct
     1381 cgatcggcag tctgcctctg ctgctggtgg cggcgcgaca cggcgctttc tgggggagtc
     1441 tcgccggggc aatcgctggt ctcctgtcgg cagccgcgca tccgtacatc gtacatccga
     1501 ttcagtttct gctcgactat cccctggcgt tcggcagcct gggtcttgcc ggatgctggc
     1561 gttggcagcc gccctggagg gcgatcgccg gcgtgacact tgccggagcg gtgaaactgc
     1621 actgccacgt ggcagccgga gtcgtatttt tttcagccgg tctgccgccc ggagcggccg
     1681 cctgggccgg cagctacttg tacaatctct cgcatatgct tcccgaaaca gtgctttgtg
     1741 caacaatcgg ctggatcctc gccgcgcgtc atccgcatct gctcattccg catacgcgag
     1801 aatagtcgtc tcgcccgtag aatcgcccca agccactgcg aacccaggac agacgcctcg
     1861 agtcgacgcg atccgcacgt cgatgccgta catggcaaaa cgccccaccg aatgggtgag
     1921 ggcgtttttg cagcgtaaac cgttgtactc cctcagctcg cggtgcggcg acggagtacc
     1981 agacctggct tcagacgatg tgggtgacct tctcgctgcc catgcagcgg gtgcagacat
     2041 accccttcgt cagcttgccg gccaccatga cctgcttgcg ctgaagattg atgttccagc
     2101 ggcgcttaga ccggcggtgg gagtgactca gctggtaccc aaccttcggc ccgcggttac
     2161 aaacgcaaca agtcctagcc atattcagcc tccaaaatcg tagcggtgaa gtggtatctt
     2221 agcagatgtt gcgtacaaat gcaataaaaa agtgtttggc ctgctgacca gaccatgtta
     2281 ccatggccac ctttcgaaga gagttctggt acaccccgcg cgaggagatt ccccatgaag
     2341 ctagcagtcg tgaccgccct ttcccggcct gttgacggaa tcgtttatct gcccctgaag
     2401 cagccagaaa gcagtgcccg caaagcgaag gctcctgcca gtcgaccgca cattcccgcc
     2461 ctgtcagagg aagaatgggc tg
//
LOCUS       DAORHD010000749         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51045_length_2481_cov_4.439406, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000749 DAORHD010000000
VERSION     DAORHD010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51045_length_2481_cov_4.439406"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PKO06_14895"
     CDS             <1..282
                     /locus_tag="PKO06_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70987.1"
                     /translation="DQKSMLGETCAGLLPDKPLVMRVGMAGYGSNPLLHTRVMGNLHV
                     VGDECSEASPGMGLMAPRVTAVASMQANQVLEILLGPDPRILEAASRKT"
     gene            290..487
                     /gene="thiS"
                     /locus_tag="PKO06_14900"
     CDS             290..487
                     /gene="thiS"
                     /locus_tag="PKO06_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNV70988.1"
                     /translation="MITVNQEPLEHADGMKVSDVLRLKNYRFPLLIIRVNGKHIPREE
                     YHSTLIPDGADVQVLHLMSGG"
     gene            complement(498..1451)
                     /locus_tag="PKO06_14905"
     CDS             complement(498..1451)
                     /locus_tag="PKO06_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNV70989.1"
                     /translation="MPDYIGTVHVFVNAEAGRGAALSRWTGLEKDLRAQLPDLKVWMP
                     KSYRELVEQVRTAAREQGSNFLAAGGDGTVQAVLDGLFDEQGKPITRQTPFVGGFGLG
                     LENRLYKPLGSQSAVGGIAARWKPEDAMEWDVIQLELVMNDGTRQIRHALQSVHLGIV
                     AAAHREVRLPSGIWKYLLNWMGFRAQALLSVWQAFRDQGFNAEIRSGDESWKGLWSGL
                     HLVKYPWVAGNFVMKTPRLPGDGKLDLAFYQKPAQPIESMTLIGTFEQQGLNQPKVTY
                     RESLSGEARLESAGTVCFDGELATIRELRWQVLPKAIRLGN"
     gene            1928..>2481
                     /locus_tag="PKO06_14910"
     CDS             1928..>2481
                     /locus_tag="PKO06_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70990.1"
                     /translation="MGLPFRFAALFCLSFFLITPPALSLSLTEALALAASHPLLLPAD
                     LAIDQEAAARLNVGARDPDTLEFEMEDFSGSFPGLRRVESTLSYRRPLLSRSLVRSRR
                     QIVETRIQRSRLEKERLQWEITTRVQNAFHRVLVNQELLETARESTLLASETFAVVAE
                     RVEAGRSPERERLQSPTERERLDGL"
BASE COUNT          496 a          787 c          651 g          547 t
ORIGIN      
        1 gatcagaaat cgatgctcgg ggaaacctgt gccggtctgc ttcccgacaa accgctcgtc
       61 atgagggtcg gcatggccgg ctacggctcg aacccgctgc tgcatacccg cgtcatgggc
      121 aatctgcacg tggtcggcga cgaatgctca gaggcttcgc cgggaatggg gctgatggca
      181 cctcgcgtga cggccgttgc ctcgatgcag gccaaccagg tactcgagat tctgctcggg
      241 cctgatccgc gaattctcga agccgccagt cgcaaaacct gaccgcgcga tgatcaccgt
      301 caatcaggaa cccctggaac atgcggatgg catgaaggtc agcgatgtcc tgcgactgaa
      361 gaactatcgt tttccgctct tgatcattcg ggtcaacggc aaacacattc cgcgcgaaga
      421 gtatcattcg actctgattc cagatggcgc ggacgttcag gtgttacacc tgatgagcgg
      481 cggttaacgt caggcggtca gttgccgagt cgaatcgctt tcggcagcac ctgccagcgc
      541 agttcacgaa tggtggcgag ttcgccatcg aagcagacgg ttccagctga ttccaggcgc
      601 gcttctccgg acaacgactc tcgatacgtg acctttggct gattcaaccc ctgctgttcg
      661 aacgtaccga tcagggtcat ggattcgatg ggctgcgccg gtttctgata gaacgccaga
      721 tcaagcttcc cgtctccggg cagccgcggg gttttcatga caaaattccc cgccacccac
      781 ggatacttca ccagatgcaa tccggaccag aggcctttcc acgactcatc accggagcga
      841 atctcggcat tgaacccttg atcgcgaaac gcctgccaga ccgacaacag agcctgagcg
      901 cgaaagccca tccagttcaa cagatacttc cagattcctg acggcagacg cacttcgcgg
      961 tgagcagcgg ccacgatgcc gagatgcacc gactgcagcg catgtcgaat ctgccgagtg
     1021 ccgtcattca tcaccaactc gagttgaatc acatcccact ccatggcgtc ttccggcttc
     1081 catcgcgcag caatgccacc aacggcactt tgactgccga gaggtttgta caaacggttt
     1141 tccagaccca ggccgaaacc accgacaaag ggggtctgcc gagtgatcgg tttgccctgc
     1201 tcatcgaaca gaccatcgag aactgcctga accgtgccgt caccaccggc agccagaaaa
     1261 ttacttccct gctctcgcgc cgccgtgcgc acctgctcaa cgagctcccg gtacgatttc
     1321 ggcatccaga ccttcagatc cggaagttgc gcacgcagat ctttctccaa cccggtccag
     1381 cgtgagagag ccgcaccgcg tcccgcctct gcattgacga aaacatgcac cgttccgatg
     1441 tagtccggca tgagtcatct cctcttcgcg tcatgaatct gtcagcatca tcataccacg
     1501 ttcgcacctt ctcttcggtc gacaaagagc gctgattttg gtatccttcg ttcatgcgtc
     1561 gttggtttat acggtggctt ttgccgttgc aactgctttc actggttttt ggtctgatcg
     1621 cggcttctct caccggatgc ccgctgaagt gctgccacca cgagagtgca acgaatcagc
     1681 cccagtatca aacgcacgat gagcacgcag acgagtctca tcatttctgc tcattcgtat
     1741 tctgcagttc aggcagtggt tttcttttgg ctgagctgca cccaccgctg cagagtcatg
     1801 ccgctgccgg catggtcatc gtccaggatg agattctgat atcccgccac cagagcgact
     1861 ttttccgccc cccgattttc gtctcctgac cttttcaccc gctcgttaat tcactcagga
     1921 gacatgtatg ggtctgccat ttcgctttgc cgctcttttt tgtctgagtt tcttcttgat
     1981 cacgccgccc gcgctgagcc tgtctctgac agaggctctg gcactggccg cttctcaccc
     2041 gcttctgctg cccgctgatc tggccatcga tcaggaagcg gcggcacgac tcaatgtcgg
     2101 tgcccgcgat cccgatacgc ttgagttcga aatggaagat ttcagcggca gctttccggg
     2161 cctgcgccgc gtggaatcga ctctttccta ccgacgcccg ctcctgtcac gctccctggt
     2221 tcggtcccga cgacagatcg tcgaaacacg cattcagcgc agtcgtctgg aaaaggagcg
     2281 cctgcagtgg gaaatcacca cgcgggtaca gaatgcattt caccgcgttc tcgtgaacca
     2341 ggaacttctc gaaaccgctc gtgaatccac tcttctggct tcagaaacct ttgcggtggt
     2401 cgccgaacga gtcgaagccg gccgttcgcc cgagcgcgaa cggctgcaat cgccgaccga
     2461 gcgcgagcgc ctggacgggc t
//
LOCUS       DAORHD010000750         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51054_length_2481_cov_3.420857, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000750 DAORHD010000000
VERSION     DAORHD010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51054_length_2481_cov_3.420857"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKO06_14915"
     CDS             <1..949
                     /locus_tag="PKO06_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70991.1"
                     /translation="AEIVAAEQRIAAVKAALDGKATAKQEAKAAETAKAAADEKKLVE
                     WAEKLAAYQASDKPGAKGRFGISTGDVEALLACRPAYEEAKALLAEFMATGINKDDHH
                     QLRQADYDIRAAIENYEGSCKRVPEGARDAVDQALKWFAGRTGATANINLDKGQAERI
                     ARLVDSAHRLAPNTPETQALLDKKAELDRLMEEADKTILQNRRMLDDKYSGGDAETLK
                     AMARSIVEKHHTGAKILKIHVTSPDWSREAAIEWTDTTRTALQNRSTLGLNAQVAVED
                     GEQALVYTVFLHKDTISGAEQPLTGHVMYKDRLLRANLP"
     gene            996..1811
                     /locus_tag="PKO06_14920"
     CDS             996..1811
                     /locus_tag="PKO06_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70992.1"
                     /translation="MSSKLRDKRRAPPSEAAEDAAEQSENDNVASERSSTLSGPKALT
                     PEAAPFPWGLVWAGLVIAVLFILRRYLRAMFPVLLLFSVFVPLLWYLWRQYKYRDLWD
                     VYRDYDELITLLHSKGLYTYGSSVDQSMRHIMAARDKAEGLLSVLERADLPGQTARIE
                     ALERQLAEVTDAEKRVSLTLKIADAQKTLEAYRRAEAFLGRYAEGKKHLAEQFRNLRL
                     KLEVPVVDPVSPSGPRVDEIDEMIQQLRRLDSTYESVDGQGQTSQPPQEFEKP"
     gene            1909..>2481
                     /locus_tag="PKO06_14925"
     CDS             1909..>2481
                     /locus_tag="PKO06_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70993.1"
                     /translation="MPQNRFPYSLHQFKQEMEPLMKRLNNCKDLDAARTELFHWVNYR
                     QREAFSKPDSKWNRNLNIIRDCARALRSLCSRHGEQTSGTSLVQTLFDIAQGRPSETL
                     TPAFFAEVTHLLRGVSLHPEPESFEMTNHFGTLQGRAAALARSDDLDELWERVEARMQ
                     RYPDGLCHEVIAFREQRKQHIMKSLGATDSD"
BASE COUNT          553 a          698 c          766 g          464 t
ORIGIN      
        1 tgccgagatc gtcgcggctg aacagcgcat cgcggcagtg aaagcggctc tcgatggcaa
       61 agccacggcc aaacaggaag ccaaagcagc cgagaccgcg aaagccgctg cagacgagaa
      121 aaagctggtt gagtgggctg aaaaactcgc ggcgtatcag gcgagtgaca agcccggtgc
      181 caagggccga tttgggatct caaccggaga cgtcgaggcc ctgctggcct gtcggccggc
      241 gtatgaagaa gccaaagccc tgctggccga gtttatggcc acgggtatca ataaggacga
      301 ccatcatcag ctgcgccagg ctgactatga cattcgagcg gcgatcgaaa actacgaggg
      361 cagctgcaaa cgagttccag agggcgcccg tgatgccgtc gatcaggcgc tgaagtggtt
      421 tgccggcaga acgggggcga ctgcgaacat caatctcgac aaaggacagg ccgagcgcat
      481 cgccaggctt gttgacagtg ctcatcgcct ggcgccgaac acccccgaaa cgcaggcgtt
      541 gctcgacaag aaagccgaac tcgatcggct gatggaagaa gccgacaaaa cgattctgca
      601 gaaccggcgt atgctcgatg acaagtattc cggcggcgac gccgagacct tgaaagccat
      661 ggcgcggtcg atcgtcgaaa agcaccacac cggcgcgaag attctgaaaa ttcatgtcac
      721 cagccctgac tggagccgtg aggccgcgat cgagtggacg gacacgaccc gcaccgcgct
      781 gcagaatcgc tccacgctcg gtctgaacgc tcaggtggcc gtcgaagatg gcgaacaggc
      841 cctggtctac accgtgtttc tgcataaaga caccatcagc ggtgctgagc agcctctgac
      901 cggtcacgtg atgtacaaag accgcctgct acgcgcaaac ctgccgtaag aaggtcgtgg
      961 tataatccga cgcaaatccc tgcgcggagc gtgttgtgag ttcgaaactg cgcgacaaga
     1021 gacgggcccc gccatctgag gcggcggaag acgctgcgga acagagcgag aacgacaacg
     1081 tcgcctcgga acgttcttca acgcttagcg ggccgaaagc tctgactccg gaagcggcac
     1141 ccttcccttg ggggctggtc tgggccggtc tggtgatcgc ggtgctgttc atcctgcggc
     1201 gctacctgcg cgccatgttt ccggttttgt tgttgttcag cgtgttcgtg ccgttgctct
     1261 ggtatctctg gcgacagtac aagtatcgcg atctgtggga tgtctaccgt gattacgatg
     1321 aattgatcac gctattacat tcgaaagggt tgtacaccta cggttcttcg gtcgatcagt
     1381 cgatgcgcca catcatggcg gcgcgtgaca aagccgaagg gttgttgtcg gtgctcgaac
     1441 gtgctgatct gcccggtcag acggcgcgca tcgaagcgct cgagcggcag ctcgccgagg
     1501 tgaccgatgc cgaaaaacgg gtttcgctga cgctgaagat cgctgatgcg cagaaaaccc
     1561 ttgaggccta tcgccgcgcc gaggcctttc tcggacgata tgcggaaggc aagaagcatc
     1621 tggccgaaca gttccggaac ctgcgcctca aactcgaagt tcccgtcgtc gatcccgttt
     1681 cacccagcgg tccgcgggtt gatgagatcg acgagatgat tcagcagtta cgccgactcg
     1741 attctacgta cgagtctgtc gacggccagg ggcagacgtc gcaaccgcct caggagttcg
     1801 aaaaaccgta ggggttgtgg cggtcggtca ggaacgtggc tctcgcgcga gccggcatgg
     1861 ctgccggcgg ggtgagggtc agtatcaagt aaaagggagt gtgacctcat gccacaaaac
     1921 cgttttccct attctctgca tcagttcaag caggaaatgg agccgttgat gaagcgcttg
     1981 aacaactgca aggatcttga tgcagctcgg acggaactgt ttcactgggt caactaccga
     2041 caacgtgagg ctttttccaa gcccgattcg aagtggaatc gaaatctgaa catcattcgc
     2101 gattgcgccc gggcattacg ctcgctgtgc agccgacacg gcgagcagac ctcgggaacc
     2161 agtctggtgc agaccttgtt tgacatcgcg caggggcgtc cgtcagagac attgacaccg
     2221 gcttttttcg ccgaagtcac gcatctgttg cgtggtgttt cgctccatcc cgagccggaa
     2281 tcgttcgaaa tgaccaacca cttcgggact ctccaggggc gcgcggcggc actggcacgc
     2341 tcggacgatc tcgacgaact ctgggagcga gtcgaggcca gaatgcagcg atacccggat
     2401 ggcctgtgtc acgaggtcat tgctttccgt gagcagcgaa aacagcatat catgaagagt
     2461 cttggggcga ccgactcgga c
//
LOCUS       DAORHD010000751         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51087_length_2480_cov_2.649485, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000751 DAORHD010000000
VERSION     DAORHD010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51087_length_2480_cov_2.649485"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /gene="flgK"
                     /locus_tag="PKO06_14930"
     CDS             complement(<1..1489)
                     /gene="flgK"
                     /locus_tag="PKO06_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNV70994.1"
                     /translation="MRATFFGFEIGKRAIFAQRAAMDVTSHNIANANTEGYARQRAVV
                     AASDPWTMPNLYSPVQGQQLGTGVVIEKIESIRNAFLDEKIVHQEQSIEQWSAANDLM
                     KQIESILNEPNDTTIRDQLDKFWAAWENLAENPNREELRSNLVEEADSLVLHLKTVDE
                     QLRRLQGTPDHSYQGSIENQLEDAVKQVNNLGLQLAELNRQIGKSETPFSQANDLRDK
                     RQRLLEELAKLVNVESQFDSRGMLTVRVGRHVLVDTIQNHDLYVVKKTKAEPGTISGC
                     PDYPQYSDTPEVCEGIVTHTGEQRNFTVAVAQTAQADSQYSFLTFHPLTGPLANFGVS
                     SGTFTVNGRTFYLDAEKTTMTDLAAMIDSANLNVHASINESGQLILDSTITGTQNAVK
                     VIDGTSNIATVLNLHVQREARDAVFTIDGKAFTTQENEVRDVMPGITLHIKKQGIANL
                     DMRPIVTSGKIKGILDVRDGQLQTLRDKLDKFVFTLVTEVNAVHRQ"
     gene            1726..>2480
                     /locus_tag="PKO06_14935"
     CDS             1726..>2480
                     /locus_tag="PKO06_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70995.1"
                     /translation="MLAASLTSPPSANSSGFARVRTALERVPALLLAFLLSLLPAAIL
                     QREVQEIRQLQRERTHQSFREQAQHTAKRVQVMTKLSYRLKDLTRRVKHEVERRIDQT
                     HLCERDLLQAEIARAMQQIKHFAPEVRVWAGILPQGDPQASPELLQGSGFETEFKTFF
                     ITLFREMLRSGHGLSNALGDEVWLPRLQNTFGNGVGNELFSAAAEGEAFAVVFRHQFH
                     IRQWNLIWRYLGPRHRPVGIMLYLEPASGTFTTL"
BASE COUNT          485 a          713 c          703 g          579 t
ORIGIN      
        1 cctgtcggtg aaccgcgttg acctcagtca ccagcgtaaa gacgaatttg tccagtttgt
       61 cgcgcagtgt ctgcagctgt ccatctcgga catcgaggat tcctttgatc ttaccactgg
      121 tcacgatcgg tcgcatgtcc aggttggcaa ttccctgttt cttgatatga agagtgattc
      181 caggcatgac atcgcgcact tcattttcct gcgtggtgaa ggcctttccg tcaatcgtga
      241 agacggcatc gcgagcctct cgctgcacat gaagattcag aacggtggcg atgttgctgg
      301 ttccatcgat gaccttgacg gcgttctgcg tgccggtgat cgtggaatcg agaatcagct
      361 ggcccgactc gttgatcgaa gcatgcacgt tgagattcgc tgaatcgatc atggcagcca
      421 gatccgtcat ggtggtcttt tcggcatcga gatagaacgt gcggccattc acggtaaacg
      481 tgccgctcga caccccgaaa ttggcgagcg ggccggtcag cgggtgaaag gtgagaaacg
      541 aatactgcga gtcagcctgc gcggtctgcg cgacggccac ggtgaaattc cgctgctcgc
      601 ccgtatgggt cacgatgccc tcgcacactt cgggagtatc agaatactgc ggatagtctg
      661 gacaaccgct gatggtgccg ggttcagcct ttgtcttttt gaccacgtag agatcgtgat
      721 tctgaatcgt atcaaccagc acgtgacgcc cgacgcgcac cgtcagcatg ccgcgtgagt
      781 cgaactgact ttcgacattc accagtttcg ccagttcctc gagcaggcgc tggcgcttgt
      841 cacgcaggtc gttcgcctgg ctgaacggtg tttcggactt gccgatctga cggttcaatt
      901 cagcgagctg cagccccaga ttgttgacct gcttcaccgc atcttcaagc tggttttcga
      961 tgcttccctg ataggaatga tcgggagtcc cctgcaggcg gcgcagttgc tcatcgacgg
     1021 tcttgaggtg caacaccaga ctgtcggcct cttcaaccag attgctgcgc aattcttcac
     1081 ggttagggtt ctcggccagg ttttcccagg cggcccagaa tttatcgagc tggtcacgga
     1141 tcgtggtgtc attcggttcg ttgagaatgc tctcgatctg cttcatcaga tcgttcgcgg
     1201 cactccactg ctcgatcgat tgttcctgat ggacaatctt ctcatccagg aaggcattac
     1261 gaatgctctc gatcttctcg atgacaacgc cggttcccag ctgttgcccc tgcacgggag
     1321 aatagagatt gggcatcgtc caggggtcgc tggccgccac cacggcacgc tgacgcgcgt
     1381 agccttcggt gttggcgttg gcgatgttgt gcgacgtgac atccatcgcg gcgcgctgcg
     1441 caaagatcgc ccgtttcccg atctcgaaac cgaaaaaagt tgcccgcatg tgtgcctcct
     1501 ggtgtgcggg caaaagcgtg tgcaaacgga tctgccttgc cttctgccgc acggccgtat
     1561 ctctgttctt ccctgaaaaa cgcagaaagg ggcaaatcct tttatcccct attatctaca
     1621 tcggccgtca ggaaggttta ccgaagccct gatttttcac gggtacggca ggaggccctc
     1681 tgcgggtatg ctacaatcag tcacccacag accttgagag ggagattgtt ggctgcttcg
     1741 ctcacgtccc caccatcggc gaactcatcc ggattcgccc gggttcgcac cgctctcgaa
     1801 cgcgtgccgg cacttctgct cgcttttctg ctgagtctgc ttccggcggc aattctgcag
     1861 cgtgaggtgc aggagatacg gcaactgcag cgcgaacgga cgcatcagag ttttcgcgag
     1921 caggcacagc acacggcgaa acgggtgcag gtcatgacca agttgtcata tcggctgaag
     1981 gatctcactc gtcgggtaaa acacgaggtg gagcgccgca tcgatcagac gcacctgtgc
     2041 gagcgcgacc ttcttcaggc cgagatcgca cgcgcgatgc agcaaatcaa acactttgcc
     2101 ccagaggtgc gtgtctgggc aggaattctg ccccagggcg atcctcaggc gagtcccgaa
     2161 ctcctgcaag gctcagggtt cgaaacagag ttcaaaacct ttttcatcac gctgtttcga
     2221 gaaatgctgc gcagcggtca tgggctgtcg aacgctctcg gagacgaggt ctggctccca
     2281 cgtctacaga acacgttcgg gaacggcgtc ggcaacgaac tcttttctgc cgcggctgag
     2341 ggcgaggcgt tcgccgtggt gtttcgtcac cagttccaca tccgacagtg gaatctgatc
     2401 tggcggtatc tcggacctcg tcatcgaccg gtcggcatca tgctgtatct tgaaccagct
     2461 tcaggcacct tcacgacgct
//
LOCUS       DAORHD010000752         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51152_length_2478_cov_3.622782, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000752 DAORHD010000000
VERSION     DAORHD010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51152_length_2478_cov_3.622782"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..979)
                     /gene="xerD"
                     /locus_tag="PKO06_14940"
     CDS             complement(44..979)
                     /gene="xerD"
                     /locus_tag="PKO06_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HNV70996.1"
                     /translation="MDHSEKKVIPFEPLLNDYLAYLSVDKGLRPKSQEAYRRDLEDLL
                     CFLHERGRSPLDGNVDAPLTMFIVHLHDQKLSPRSIARKVSAMRGFFRFLVREGRILE
                     DPSKLLERPKTGHPLPKVLSVDEVTRMLEQPDLTNARGLRDRAMLEVLYGAGLRETEL
                     IEMTLDQVHFEGEYLQILGKGGRERVVPIGRYALNAIRAYLEHARPRLLRDITIRTLF
                     LNPRGRPFSRMGVWKLVRQYAIAAGIPREISPHVLRHSCATHMLEGGASILVVQEMLG
                     HVDISTTQIYTHLSRQDLIRIHRQAHPRSGAGGSS"
     gene            1445..>2478
                     /locus_tag="PKO06_14945"
     CDS             1445..>2478
                     /locus_tag="PKO06_14945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV70997.1"
                     /translation="MASFAAEELPISKAVALALEYSPFMKAMTAGSQVAREKIGEAQA
                     MNRAKIAVGVTDSTTDSPMLAFGSKLNQQRITAADFDPARLNDPDYTNNLQAGLQVVQ
                     PLYLGGMDRHAVSAARKGLQAQELDREQATQDVIFRTIEAYLNVILARESVSVAERAV
                     EASKESLRNTSAAYEANQAVQSDVLQAQVHHSQNEETLLRMQNQYRLAREGLATVLGV
                     TTVEEYRLNMPFLQQECQTCKEEAGELLKEALAERSDYRKLSHQMEAAGHMEKMARGT
                     VRPHVAIGTRLEHNSRDLSRSGHANALFFARMDWNIGDGGEARHKARGAKWQAAQLAD
                     FMKAKEDQIRL"
BASE COUNT          570 a          713 c          689 g          506 t
ORIGIN      
        1 aatttgtcgc gctcgtctca tggtggcgct ctcgtttcgg tggtcacgac gaacctccag
       61 ccccactgcg cgggtgcgcc tgtcgatgaa tgcgtatcag atcctgccgc gacagatgcg
      121 tgtagatctg tgtcgtcgaa atatccacgt gtcccagcat ttcctgaacc accagaatgc
      181 tggctccacc ctcgagcata tgcgtggcac aggaatggcg gagcacgtga ggtgagattt
      241 cacgtggaat gccggcggca atcgcatact gtcgcaccag cttccagaca cccatgcgtg
      301 aaaatggacg gccgcgggga ttgagaaaaa gcgtgcgaat agtaatgtca cgcaacagcc
      361 tgggacgcgc gtgctcgaga tacgcgcgaa tggcgttcaa cgcatatcta ccgatcggca
      421 cgacgcgttc gcgaccaccc ttcccgagaa tctgcaggta ttcgccttcg aagtgcacct
      481 gatcaagcgt catttcgatc agttccgtct cgcgcaagcc cgctccgtac agcacttcga
      541 gcatcgctcg atcacgcaga ccgcgggcgt tcgtcagatc aggctgttcg agcattcgcg
      601 tcacctcgtc gaccgacaac accttgggga gcggatgccc ggtctttggc cgttcgagca
      661 acttcgaggg gtcttccaga atgcggcctt cgcgaaccag aaagcggaaa aagcctctca
      721 tagccgagac tttgcgggcg atggagcgag gagaaagctt ctggtcatgc agatgaacga
      781 tgaacatcgt cagcggtgca tccacatttc catcgagtgg cgagcgtccg cgttcgtgga
      841 ggaaacagag caggtcctcc agatcacgac ggtaggcttc ctgcgatttc ggccgcagcc
      901 ccttgtccac cgacagatac gccagatagt cgttcagcag cggctcgaag ggaatcacct
      961 tcttttccga gtggtccatc ttcctacata tcggaattcc ccatcaaaaa cgtgagtttc
     1021 ttcgcttcca cggccgctca tcgagggtat aggatccatt gtcagacgca cgcttcctgc
     1081 tcgcgcattc tttgccatgc aatatttgca ttctcgttcc tgctgtttcg cgcactcgtg
     1141 tggaaactct atccagcagc ggcattcaca agatgcgcat ttcttggcac agcaattgca
     1201 aatgcgacgg cgtgactcaa cgaaactcgc tcgactcgcc tggatctatg catgtccctc
     1261 aatcttatga gcagcaggag gtgcattgca gaatcgcaac gatttcctgc gagattgcag
     1321 tcgcactcac ctttcaacta tgatgatcgc atctcaaccc aaatgacttt gtgaccctgg
     1381 aggaactttc gatgagaggt ttgtcacttg tttccggctc gccgcactga tgatactgat
     1441 atcgatggcg tcgttcgctg ccgaggaact cccgatctcg aaggccgtag cgctcgctct
     1501 cgagtacagt ccgttcatga aggccatgac agccggttcc caggttgccc gggaaaagat
     1561 cggcgaagcc caggccatga atcgcgccaa aatcgccgtc ggtgtaaccg acagcacgac
     1621 cgacagcccc atgctggcat tcggcagcaa actcaatcag caacgcatca cggcggcaga
     1681 tttcgatccg gccaggttaa acgacccgga ctatacgaac aatctgcagg ccggacttca
     1741 ggtggtgcaa ccgctgtatc tcggtggcat ggaccggcac gccgtctcag ccgctcgaaa
     1801 agggcttcag gcacaagagc tcgatcgcga gcaggcgacg caggatgtca ttttccgaac
     1861 gatcgaagcc tatttgaacg tcattctcgc tcgtgaaagt gtaagcgtgg ccgagcgagc
     1921 cgtagaagct tcaaaggaaa gcctgcggaa cacttctgcg gcatatgagg cgaaccaggc
     1981 cgtgcagtct gatgtgctgc aggctcaggt gcatcactcc cagaacgaag agacgctgtt
     2041 gcgcatgcag aatcagtatc gcctggcccg ggaaggtctg gcaaccgttc tcggagtcac
     2101 aaccgtagaa gagtatcgcc tgaacatgcc gtttctacag caggaatgcc agacttgcaa
     2161 ggaagaggcc ggagaactgc tgaaggaagc attggccgag cgttctgact atcgcaagct
     2221 ctcgcaccag atggaagcag ccgggcacat ggagaagatg gcccgaggca cggtgagacc
     2281 gcatgtggcc atcggaaccc gactagaaca caacagtcgc gatctttctc gcagcggtca
     2341 tgcgaacgcc ctcttcttcg cacggatgga ctggaacatc ggcgacggtg gtgaagcacg
     2401 gcataaagcc cggggagcga aatggcaggc tgcccagctc gccgatttca tgaaagcaaa
     2461 agaagatcag attcgtct
//
LOCUS       DAORHD010000753         2474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51280_length_2474_cov_4.076064, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000753 DAORHD010000000
VERSION     DAORHD010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51280_length_2474_cov_4.076064"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PKO06_14950"
     CDS             <1..522
                     /locus_tag="PKO06_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV70998.1"
                     /translation="DCDLEEVKPVKLLIKIDLEHRRRTQTIEKVEVKNASITPGGILE
                     VWVTMRPFREKPVIRKARLPIPADFGKENLRLTVHGLGSRGVDTEETETGEAKAAKGK
                     DVKKDEPAHENFNTLASSWLNKPRNSDLIFQLSSEADAEKKYKISGKDFETMATNLVV
                     IGSIDTTITLSED"
     gene            524..1315
                     /locus_tag="PKO06_14955"
     CDS             524..1315
                     /locus_tag="PKO06_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV70999.1"
                     /translation="MFPSSLMQDLFSFFGSLMILFLQTLYWTLYNLRVGRFGFKNLMD
                     QMSRIGVDSLPMVLITGFSTGAVLVVQIGYQFTQLGAERYVGGVVSMAMARELAPILT
                     AIVVAGRVGSSIAAELGTMKITEQVDALETMAANPVEYLVVPRFWGCTIMLLLLTIFT
                     NVIGMIGGSIVAVYQIGLTLTTFQDSILQNLKIFDLMGGLFKGFVFGGIIGIIGCYKG
                     FTTEGGAEGVGKSTTGAVVLAIMLILAVNYFLTVGINNVQTNYFQ"
     gene            1312..2058
                     /locus_tag="PKO06_14960"
     CDS             1312..2058
                     /locus_tag="PKO06_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71000.1"
                     /translation="MIIISNLNKKFGTKEVLRGINLKIYPGESLVILGPSGCGKSVLL
                     KHIIGLLKPDSGQVLFDGEDITRFGQRKLDQLRMRMGMLFQSAALFDSLTVEENVAFG
                     LRKHTNLGHTAIHHIVSEKLEMVGLPGTNHLMPSELSGGMRKRVGLARAIAMDPEIIL
                     YDEPTTGLDPIMTSAIDELHVKLKRELGVTSIVVTHDLNSAYRICDRLALHYGGQIVA
                     IGTKEEFVHSPNPLVQQFIRGEAKGPMTLL"
     gene            2071..>2474
                     /locus_tag="PKO06_14965"
     CDS             2071..>2474
                     /locus_tag="PKO06_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV71001.1"
                     /translation="MQSTIKVGIITLIGLALLVYMVFIIGDFRLSEAGYKFYITFYSV
                     NGLSQGASVSMAGVKIGKVERVEIRDDQVFVRVYIRNQAVRIRRRSTFTIGTAGLMGE
                     KFVDIIPTRDQTSPYVIADGQVEGTDPTRMEEL"
BASE COUNT          557 a          687 c          680 g          550 t
ORIGIN      
        1 gattgcgacc tcgaagaggt caagcccgtg aagctgctga tcaagatcga tctcgaacat
       61 cgacgtcgca cccagacgat cgagaaagtg gaagtgaaga acgcctcgat cactccgggc
      121 gggattctgg aagtctgggt caccatgcga ccgttccggg aaaagcccgt gatccgcaag
      181 gcgcggctcc cgattcctgc cgatttcggc aaggaaaacc tgcgattgac ggttcatgga
      241 ctcggcagtc gaggagtcga cacggaagaa acagaaaccg gtgaagcgaa agccgccaag
      301 gggaaagatg tgaagaaaga cgagccggcg catgagaact tcaacacgct ggcgtcttcg
      361 tggctgaaca aaccgcgcaa ctctgatctg atcttccagc tgtcctctga ggccgatgcc
      421 gagaagaaat acaagatttc tggcaaggac ttcgagacga tggcgacgaa cctcgtggtc
      481 atcggcagta ttgacaccac catcacctta agcgaggact gatatgtttc cctcgagtct
      541 gatgcaggat ctcttcagct tcttcggaag cctgatgatc ctgttcctgc agacgttgta
      601 ctggacgctc tacaacctgc gagtcggcag attcggcttc aagaacctga tggaccagat
      661 gtcgcgcatc ggagtcgatt ctctcccaat ggtgctcatc accggcttct caacaggcgc
      721 cgttctggtg gttcagatcg ggtatcagtt cacgcaactc ggtgctgaac gctatgtcgg
      781 tggtgtcgtc agcatggcca tggcgcgcga gctggcgccg attctgaccg cgattgtggt
      841 cgccggccgc gtcggcagct cgatcgccgc tgaactcggc acgatgaaga tcaccgagca
      901 ggtcgatgct ctcgaaacca tggcggcgaa cccggtcgag tatctggttg tgccgcgctt
      961 ctggggctgc accatcatgc tgctgctgtt gacgattttc accaatgtca tcggcatgat
     1021 tggtggttcc atcgtcgcgg tgtatcagat cggccttacc ctgaccactt ttcaggattc
     1081 gattctgcag aatctgaaga tcttcgacct gatgggcggg ttgttcaaag gttttgtttt
     1141 cggtggcatt atcggcatca tcggctgcta caagggcttc accaccgaag gaggtgccga
     1201 gggcgtcggg aagagcacga ccggagctgt cgttctggcc atcatgttga ttctcgccgt
     1261 gaactacttc ctcaccgtcg gtattaacaa cgttcagacc aactacttcc aatgatcatc
     1321 atttccaatc tcaacaaaaa gttcggcacc aaagaggtgc tgcgagggat caacctcaaa
     1381 atctatccgg gtgagagtct ggtgattctg gggccttcgg ggtgcggaaa aagcgttctg
     1441 ttgaagcaca tcatcggcct gctcaagcct gacagcggcc aggtactgtt cgatggcgaa
     1501 gacatcacgc gcttcggcca gcgcaaactc gatcaactgc gcatgcgcat ggggatgctg
     1561 tttcagagcg ccgctctttt cgactcactg accgtcgaag aaaatgttgc cttcggtctg
     1621 cgcaagcata cgaatctggg ccatacggcc attcaccaca ttgtctcaga aaagctcgaa
     1681 atggtcggat tgcccggaac aaatcacctg atgccatcag agctgtcggg gggcatgcgc
     1741 aagcgcgtcg gcctggcccg tgcgatcgcc atggatccgg aaatcattct ctatgatgag
     1801 ccaaccaccg gtctcgaccc gatcatgacc tctgccattg acgaactgca tgtcaagctc
     1861 aaacgagaac tcggtgtcac ctcgattgtc gtcacgcatg acctgaattc ggcttatcgc
     1921 atctgcgatc ggctggcact gcattatggt ggacagatcg tcgcgatcgg caccaaggaa
     1981 gagttcgttc attcccccaa cccgctggtt caacagttta tccgtggtga ggccaaaggc
     2041 cccatgaccc tcctgtaagg agccctgcac atgcaatcaa ccatcaaagt cggtattatc
     2101 accctgatcg gcctggccct gctggtctac atggtcttca tcattggtga tttccgcttg
     2161 agcgaagccg ggtacaagtt ctacatcacc ttctattcgg tcaatggcct gagtcaaggg
     2221 gcatcggttt cgatggccgg cgtcaagatc ggcaaggtcg agcgcgttga gattcgcgac
     2281 gatcaagtat ttgtccgagt ttacattcgc aaccaagccg ttcgaatcag acgtcgcagc
     2341 actttcacca tcgggaccgc cggtttgatg ggtgaaaaat tcgtcgatat cattccaacc
     2401 cgcgatcaga catcgccgta tgtcatcgcc gacggccagg tcgaaggcac cgatccgacc
     2461 cgcatggaag aact
//
LOCUS       DAORHD010000754         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51354_length_2471_cov_4.482616, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000754 DAORHD010000000
VERSION     DAORHD010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51354_length_2471_cov_4.482616"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1789
                     /locus_tag="PKO06_14970"
     CDS             <1..1789
                     /locus_tag="PKO06_14970"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71002.1"
                     /translation="FEGLAGWRHHGGNCYFHQLPEPLEIDDADRAGATLYENGRALGP
                     GHQNVDTIRRGGGAFFLSDTGTLFFSTSDGTSPITNCREYVVALPVSGRTFVLDRGDP
                     GHVSAVNLAKLESAWRQGETSVDGLPSHVAWVSSLDCNLQCPMCSQNYARWNVQKAVP
                     GLGKQVLSMVPALLHLTWNGGEPWIDPEFIGFIERFQTNDNPNLTFGFTSNGVLIGER
                     ERGWLEKFSRWNISISVDSFQPRTYARLRRGAELQQVLETLRALQKRQRLPHRRLSVG
                     ALVMKSTIRELPDNLQQALDLDLSINWSPVTVYPPTEQLNLFSNFADETRGWREAIDR
                     ADLIAGDAVQTRLASVRRIDPRFMIKHIGQLLSQAESEYAQTVPLRVGCQDPENLLAH
                     LTEPGLVVLREAEPIAYLALRADAEQILALPLSRLQTGAGRLYWVLTANLRNLLQQAF
                     LAPPRLLPSLAGPGEIRLNIRLFANELTTDPAFSLWPLAAPFSRERDSCWVTGLPAVP
                     TPGDSMTAPFQSRLQLFENGRAIGLAHAVHEDIMRQGLGRFSHWHDRLFFSTSDNSDP
                     NRNGRRYAVQVWPEVEMIRVPPTLFSRRA"
     gene            1786..>2471
                     /locus_tag="PKO06_14975"
     CDS             1786..>2471
                     /locus_tag="PKO06_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71003.1"
                     /translation="MNGPSRAALVILLLVLATVMSRFQYDPERAPKVRLTLDTSQIRL
                     ETAGSHAYVYRGIASTVGTGDTARFPRWSRMELLENGLKLSLPHSLHADIRSKGDGRF
                     SHWNEDLIFSSSDGSDPRTNGRVYEVSCPRPPGWRERTATFGLWLSLLAAFITALPVA
                     GQVCCRIIPGLGAAIAFGGLALFLVVPLVIPCAPTQAWAWIRGRHLLYLTVGQMAGWL
                     WIWAMCGVGCA"
BASE COUNT          453 a          713 c          752 g          553 t
ORIGIN      
        1 gttcgaaggt ctggcggggt ggcggcatca cggggggaac tgctattttc atcagcttcc
       61 cgagccgttg gagatcgatg acgcggatcg agcgggagct acgctgtatg agaatgggcg
      121 agcgcttgga cccggacacc agaatgtcga tacgattcgc cgcggcggcg gggcattctt
      181 tttgagtgat acaggaactc tgtttttctc gacctcagat ggtacgagtc cgatcaccaa
      241 ttgccgggaa tacgtcgtcg ccctgccagt ctccgggcgc actttcgtgc tcgatcgcgg
      301 tgatccgggt cacgtatcag cggtgaatct tgcgaaactc gagtcagcct ggcggcaggg
      361 ggaaacgtcc gtcgatggat tgccatctca cgtggcctgg gtttcatccc ttgattgcaa
      421 cctgcaatgc ccgatgtgca gtcagaacta tgcgcgctgg aacgttcaga aagcggttcc
      481 cggacttggc aaacaggtac tctcgatggt gcctgcgttg ctgcatctca cgtggaacgg
      541 tggtgaaccc tggattgacc ccgagttcat tgggtttatc gagcgttttc agacgaatga
      601 caacccgaac ctcaccttcg gattcacatc gaatggagtc ttgattggcg agcgcgaacg
      661 cggctggctc gagaagtttt cgcggtggaa catttcgatc tcggttgaca gttttcagcc
      721 gcgaacctac gcccgcctga gacgaggagc ggaactccag caggttctcg agactctgcg
      781 agcgctgcag aaacgccaac gcctgccgca tcgacgtctg tccgtgggag cgctggtcat
      841 gaaatccacc atccgcgaac tgccggacaa cctgcaacag gctctggatc tcgatctttc
      901 gataaactgg agtcccgtga ccgtctaccc gccaaccgag caactcaacc tgttttcgaa
      961 cttcgctgac gagacacgag gctggcgcga agcgatcgac cgtgccgatc tgattgccgg
     1021 tgatgcggtt cagactcgcc tcgcctctgt tcgtcgaatt gatcctcgtt tcatgatcaa
     1081 gcatatcgga caattattga gtcaggccga aagcgagtac gcgcagacag ttcctcttcg
     1141 ggttggctgt caagacccgg aaaacctgct cgcccatctt acggagcccg gcctggtcgt
     1201 gttgcgcgaa gcagaaccga tcgcctatct tgcgctaaga gcggatgcag agcagatact
     1261 cgctcttccc ctgtcgcgac ttcaaaccgg cgcgggtcgc ctctactggg tcctgactgc
     1321 caatctgcga aatctgcttc aacaggcgtt tctggcgccg ccccgcttgt tgccgtcgct
     1381 tgccggcccg ggcgagattc gtctgaacat caggttgttc gcgaatgaac tcacgacgga
     1441 cccggctttt tcactctggc cgctggcagc gccgttctca cgggaacgcg actcctgctg
     1501 ggtgaccggc ttgcccgcgg tgccgacgcc gggcgactcg atgaccgctc cgtttcagtc
     1561 cagactgcag ctcttcgaga atggacgagc aatcggcctg gcacacgctg tccatgaaga
     1621 tatcatgcgg caggggctcg gacgattttc acactggcac gatcgactgt tcttctccac
     1681 gtctgacaac tccgatccga atcggaacgg tcgtcgctat gccgtgcagg tctggcctga
     1741 ggtcgaaatg attcgcgttc ccccgacgct tttttcccgg cgcgcatgaa cggtccgtca
     1801 cgcgcggcgc tggtcatcct tctgctggtg ctggcgaccg tcatgtcacg ctttcagtat
     1861 gatcctgagc gggccccgaa ggttcgactc acgctcgata cgtctcagat caggttggaa
     1921 accgccggtt cgcatgccta tgtctatcgc ggaatagcat ccacggtagg aacgggcgac
     1981 accgcgcgct ttcctcgatg gagtcgaatg gagctgctcg aaaacggatt gaagttgtcg
     2041 ctgccgcaca gcctgcatgc cgacatccgc tcaaaaggag atggcagatt ctctcattgg
     2101 aacgaggatc tgatattttc cagctcggac ggaagtgacc ctcgcaccaa tggtcgggtg
     2161 tatgaggtgt cttgtccgcg gcctccgggg tggcgagagc gaactgcgac gttcgggctg
     2221 tggctgagtc tgttagccgc gttcatcacg gcacttccgg tcgcgggtca ggtatgctgc
     2281 cggatcatac cgggattagg cgcggcgatc gcgttcggtg ggctggcgct gtttctcgtg
     2341 gtgccgctcg tgattccatg cgcgcctacg caagcctggg cgtggatcag agggcgtcat
     2401 ctgctgtatc tgacggtcgg tcagatggcg gggtggctgt ggatctgggc catgtgcgga
     2461 gttgggtgcg c
//
LOCUS       DAORHD010000755         2470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51385_length_2470_cov_3.873292, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000755 DAORHD010000000
VERSION     DAORHD010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51385_length_2470_cov_3.873292"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PKO06_14980"
     CDS             <1..649
                     /locus_tag="PKO06_14980"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV71004.1"
                     /translation="VALEQFEAEALALLDLLGEIVSRHGICQLRLGESEKFAYVTRFF
                     NGGREEPFPLEERMEISSPKVKSYDQKPEMAAFEIGDALMERIQTQKYGLIITNFANG
                     DAVGHTGNVAAAMKAVSTVDSVLSKLIPLAYDLGYDCVITSDHGNVEQMIDERTGEPF
                     PDNTLNPVPFCVLSREFRDLRSEGALADIAPTILEMLGLEKPSCMDGVSLINRKK"
     gene            649..1248
                     /gene="gmk"
                     /locus_tag="PKO06_14985"
     CDS             649..1248
                     /gene="gmk"
                     /locus_tag="PKO06_14985"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015903734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV71005.1"
                     /translation="MTQTHEMGVTPGRLFVVSGPSGAGKTVLCARMLEEFKGQMVLSI
                     SATCRSPRGAEVDGRDYFFYPRAEMERLIRENQLVEWALVHDHYYGTPRAFLEENRKR
                     GTHVILNIDVQGARQLRQAYPDAVTFFIAPPSLDVLEQRLRMRKVDAEAVLQKRMANA
                     RLEMSCEKEYQHVIINDVLDRAVNELATLIRLSLSRSEA"
     gene            1319..2131
                     /locus_tag="PKO06_14990"
     CDS             1319..2131
                     /locus_tag="PKO06_14990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV71006.1"
                     /translation="MNTRSMLSFLLMSFLVVTTLAFGADDVATPLDVKLGIVAGQVKL
                     TRVGKTLSDLYSQGFPVYHGDIIETMRESKAELQYSDGTIMRLKPLTKVEIQATSLKV
                     FKGQSWFKFTKRGSEFVVETPSLVAGIRGTVFDVAVTSRGKSVVSVMQGRVAVRGKKG
                     GDEVLLDQGKASHCEIGKEPVKAYDFDAEKKSDEWLEVDWDAMGVHNSDAQTRYITYL
                     NLKSEYGENDSRTVEALKLFEQARKAKKPDQVLPKPAKKNDKKNPKSAKKAE"
BASE COUNT          544 a          638 c          742 g          546 t
ORIGIN      
        1 agtagccctg gaacaattcg aagccgaggc gcttgcattg ctcgacctgc tcggggagat
       61 tgtttcccgc cacggaatat gccagcttcg tctcggggaa agcgaaaaat tcgcctacgt
      121 gacgcgtttt ttcaacggtg gtcgcgaaga gccttttccg ctcgaagaac gtatggagat
      181 ctcttctcca aaagtgaaaa gttacgatca aaagcccgag atggctgcct ttgaaattgg
      241 ggacgccttg atggagcgca ttcagacgca gaaatacggg ctgatcatca cgaactttgc
      301 taacggcgat gcggtgggac acaccgggaa tgtggcggca gccatgaaag cggtgagtac
      361 cgtcgacagt gttctgtcga aactcattcc gctggcctat gatctaggct acgactgtgt
      421 catcacctcc gaccacggaa atgtcgagca gatgatcgat gaacgaaccg gtgagccgtt
      481 tccggacaat acattgaatc cggttccgtt ttgcgttctc tcgcgagagt tccgcgatct
      541 gcgttcggaa ggcgcgctcg cggacattgc ccccacgatc cttgagatgt tggggctcga
      601 gaagccgagc tgcatggacg gcgtttctct gatcaaccgc aagaagtgat gacgcagact
      661 cacgagatgg gagtcactcc cggtcgactg ttcgtggtct ccggcccttc gggagccggt
      721 aagaccgtgt tgtgcgcaag gatgctcgaa gagttcaagg gacagatggt cctctcgatc
      781 tcggcaacct gccgttcccc ccgcggggcg gaagtggatg gtcgcgatta ctttttttat
      841 ccacgtgccg aaatggaacg tttgattcgc gaaaaccagc ttgtggaatg ggcgcttgtc
      901 cacgaccact attatggaac cccgcgtgcg tttctcgaag agaaccggaa aaggggaacc
      961 cacgtcattt tgaacatcga cgtgcagggg gctcggcaac tgcggcaggc gtatcccgat
     1021 gcggtcacgt tcttcattgc accaccatct ctcgacgttc tcgaacaacg actgcgtatg
     1081 cgcaaggtcg acgccgaggc tgtgctgcag aagcggatgg cgaacgctcg tctggagatg
     1141 agttgtgaga aagagtacca gcatgtcatc atcaatgatg tgctcgatcg ggcggttaac
     1201 gaactcgcca ctctgatacg tctcagcctt tcccggtcag aggcttgacg aagcagcgct
     1261 tcgttcctat aattttgaca tacctttttc aaggcaatct gctgctctgg aggcaagtat
     1321 gaatacgcgg agtatgttga gcttcctgtt gatgtcgttt ctggtcgtca ccacgctggc
     1381 atttggtgcc gatgatgtcg ccacacctct tgatgtcaag ctgggtatcg tcgccggaca
     1441 ggtcaagctg actcgggtgg gcaagacgct ttctgacttg tacagccagg gttttccagt
     1501 gtatcacggt gatatcattg agacgatgcg tgaatcgaaa gccgaactgc aatacagcga
     1561 tggcacaatc atgcgtctga agccattgac caaggttgag atccaggcga cttctctgaa
     1621 agttttcaag gggcagtcct ggttcaagtt taccaaacgt ggctctgagt tcgttgtcga
     1681 gacccccagt ctggtcgccg gcattcgcgg caccgtgttt gatgttgccg ttacctcccg
     1741 cggaaagtcc gtggtttcgg ttatgcaggg gcgcgtcgcg gttcgtggaa agaagggtgg
     1801 cgacgaagtg ctgctcgatc agggcaaggc ttcgcactgt gagattggca aagagccggt
     1861 gaaggcctac gactttgatg ccgagaaaaa gtctgatgag tggctcgaag tagattggga
     1921 tgccatgggt gtgcacaata gtgacgcaca gacccgctac atcacgtatt tgaacctgaa
     1981 atccgaatat ggggaaaacg attcacgcac ggttgaagcc ctgaagctgt tcgaacaggc
     2041 gcgcaaggcg aaaaagcctg atcaggtgct gccgaagccg gcgaagaaga acgacaagaa
     2101 gaatcccaag agcgccaaaa aggccgagta agcgtttttt caggcgtcac tcgcgacggc
     2161 cccgtgtctc agacatgggg ccgtcgtttt tggggtgccg gtggacgcga agcgctttct
     2221 cagtttggga gcacatctgc aggactcgag agcctaaccg gggcgatgcc tgcgcgtgag
     2281 cgctctctca taaccggcga ctgacgcccg gtttcggcca atcgcacgcc gaaggcgaag
     2341 ctgtgagaca gaactgccgc ttactgccgg agtggctttg ctcgactctc gttgtttccg
     2401 acgaggggtg ccactgcggg agtgccctgc agcacagcac tcggccgagc gggcgattgg
     2461 acggccggac
//
LOCUS       DAORHD010000756         2467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51499_length_2467_cov_2.606551, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000756 DAORHD010000000
VERSION     DAORHD010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51499_length_2467_cov_2.606551"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2467
                     /locus_tag="PKO06_14995"
     CDS             <1..>2467
                     /locus_tag="PKO06_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71007.1"
                     /translation="FFADLAKYGQLAYSDRALWLSVLGNTFFWFLASLFFTNIPVFGM
                     TVLGLAKEQLAALFVALTVGIGLGSFLAGHLSGNKIEYGLIPLGAILMTAFSFYLSVC
                     TSWGHTVFAMAMVGFGSGFFIVPIQALIQYRPEPANKGGLQGLSYFMSNVGTISASLF
                     FYVITVWFSISASSIFLISGVLTVGVTAYVLWLLPDSLIRMVLWLLTHTFYRIRVVGR
                     DHIPDRGGALFVANHLSFVDSLLVIASTDRFVRFIMHREYYEQIWIKPLAKIMGVIPI
                     AGNDGPRALLQSLRDASKSIENGDVVCIFAEGQMTRTGLMLPFRRGYERIMKGLSHPI
                     IPIHLDSIWGSIHTFERGRFFWKWPRTFPFPYPITVSYGKPMPADTSAPVLRSVVQEL
                     STEAFTYRKDEMQPLHRAFIGEARLHPFRLAMADVQNEYVSTFSALIRSIALSRALRE
                     TWRGQEMVGLMLPTSVSAAIANIAVLLAGKVPVNLNYTASQDSVNYAAKLCGIKTVLT
                     ARALLEKVKVGVPAETTGVILEDLAPTITRGDKLIATLMALCFPKSMIEWALGSTKTW
                     TLEDMATVIFSSGSTGEPKGVMLSHYNIYANLEGIAQILRVEKEDGILGALPLFHSLG
                     FTGTFWFPLLKGFRVAYHPNPIDAKTIGSLVEKYWLTILCATPTFLQAYMRRITPEQF
                     GSLRYVMVGAEKMPERVAVAFEDKYGIRPHEAYGCTECAPAVTMSVPGFRGPGFYQVG
                     SKRGAIGHPLPGITVRITDPDTGAPLGTGKPGLLWVKGPNIMIGYLGKPEKTSEVLKD
                     GWYMTGDIAVLDEDGFLTITDRLS"
BASE COUNT          452 a          748 c          717 g          550 t
ORIGIN      
        1 ttcttcgccg acctggcaaa gtacgggcag ctggcgtatt cagaccgggc tctctggctg
       61 tcggttctgg gcaacacctt tttctggttt ctcgcctcgc tcttcttcac gaatattccg
      121 gttttcggca tgacggtact cggactcgcg aaggagcagc tggcggcatt gttcgtcgcg
      181 ctcacggtcg gtattggcct gggcagcttt ctcgccggcc atctttccgg gaacaagatc
      241 gagtatgggc tgattccgct cggagcgatt ctgatgacgg ccttctcgtt ctacctctcg
      301 gtttgtacca gctggggaca cacggtgttc gccatggcca tggtcgggtt cgggtcaggc
      361 ttcttcatcg taccaattca agccttgatt cagtatcgac cagaaccggc gaacaagggc
      421 ggcttgcagg gcctgtcgta tttcatgagc aacgtcggca ccatttccgc gtcgttgttc
      481 ttttacgtga tcaccgtctg gttcagcatc tcggcgtcat ccatctttct gatcagcggc
      541 gtgctcacgg tcggcgtgac cgcgtacgtg ttgtggctgc tgcccgactc actgattcgc
      601 atggtgctgt ggctgctgac acacacgttt tatcgaattc gggtcgtcgg tcgcgaccac
      661 attcccgatc gcggcggcgc tctgttcgtg gcgaaccatc tttcctttgt cgattcgctg
      721 ctggtcattg cctcgaccga ccgcttcgtg cgcttcatca tgcaccgcga gtactatgag
      781 cagatctgga tcaagccgct ggccaagatc atgggggtca ttcccatcgc cggaaacgac
      841 gggccgcgcg cgctgctgca atctctgcgc gacgccagca agtcgatcga aaacggagat
      901 gtggtttgca tcttcgctga ggggcagatg acgcgcaccg gcctgatgct gccattccgc
      961 cgcggatatg aacgcatcat gaaagggctg tcccatccga tcattccgat tcacctcgat
     1021 agcatctggg gctcgattca caccttcgaa cgtggccgct tcttctggaa atggccgcgc
     1081 accttcccgt tcccctaccc gatcacggtt tcctacggca agccgatgcc tgccgacacc
     1141 tccgctcccg tgctgcgttc cgtcgtgcag gagctctcga ccgaggcgtt cacgtatcgc
     1201 aaagacgaga tgcagcctct gcatcgtgcc ttcatcggtg aagcgcgcct gcatccgttc
     1261 cggctcgcca tggccgacgt acagaacgag tatgtctcga cgttcagcgc actcattcgg
     1321 tcgatcgcgc tgtcgcgcgc tctgcgcgaa acctggcgcg gtcaggagat ggtcgggctg
     1381 atgctgccaa ccagcgtctc ggcagccatc gccaacattg ccgttctgct ggccggaaaa
     1441 gtgcccgtca atctgaacta caccgcctcg caggattccg tgaactacgc agcgaagctg
     1501 tgcggcatca agacggtgtt gacggcccgc gctctgctcg aaaaagtgaa agtcggggtt
     1561 ccggccgaga ccacgggcgt gattctggaa gacctggcac cgacgatcac gcgcggcgac
     1621 aagttgatcg ctactctgat ggcgttgtgt ttcccgaaat cgatgattga atgggcattg
     1681 ggctcaacca aaacctggac actcgaggat atggccacgg tcatcttctc gagcggcagc
     1741 acgggtgagc ccaagggagt catgctcagt cattacaaca tctacgccaa cctcgaaggc
     1801 atcgctcaga ttctgcgggt cgagaaggaa gacgggattc tcggagcgct gccgctgttc
     1861 cattctctgg gcttcaccgg cacgttctgg ttcccgctcc tgaagggctt tcgcgtcgcc
     1921 tatcacccga acccgatcga tgcgaaaacg atcggttctc tggtcgagaa atactggttg
     1981 acgatcctgt gcgccacgcc gacctttctc caggcgtaca tgcgccgcat tactccggag
     2041 cagttcggct cgttgaggta tgtgatggtc ggtgcggaaa agatgccgga acgcgttgct
     2101 gttgcgttcg aggacaagta cggcattcgg ccgcacgagg catacggctg caccgaatgt
     2161 gcgcccgcgg tcaccatgag cgtacccggg ttccgcggcc ccgggttcta ccaggtgggt
     2221 agcaagcgcg gcgcgatcgg acatccgctg cccggcatca cggtcaggat caccgacccg
     2281 gacaccggcg cgccactcgg caccggcaag cccggactgt tgtgggtcaa aggtccgaat
     2341 atcatgatcg gctatcttgg aaagcctgaa aagacaagcg aagtgttaaa agatggctgg
     2401 tacatgaccg gcgacatcgc cgtgctcgac gaagatggat ttttgaccat caccgaccgc
     2461 ctctcgc
//
LOCUS       DAORHD010000757         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51531_length_2466_cov_3.294069, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000757 DAORHD010000000
VERSION     DAORHD010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51531_length_2466_cov_3.294069"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PKO06_15000"
     CDS             <1..1047
                     /locus_tag="PKO06_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71008.1"
                     /translation="TTMYSAVSLLVRARDALNRGEIETSRKFVDEILVREPHYTEAQD
                     LRTEIDKQVELKELVSSVDEQMSRGRLAEALESANELIKKYPAQALFYVLRARIHLKT
                     HRFRDAQSDLREARLLGYDFAQIRSYLVDVYAGQGLFAQAYALAQGNAECSPCRPWDT
                     LWIWYFKSSFFGWIIFCASLVVAGGTFFVFWRQFDALIGRYSLSHFFSVAACLYQCST
                     NGATSQIERLISITKRMPHPWWLYVTALSLMEIGQTDRSQELLLRAAAAPELSARAHL
                     YLGLIRGESEPRLADYDLEQVVICCQTEVEHPWLPDFLQTLEQSVLDRYRERLKNHRF
                     LELAIESFHGVQST"
     gene            1070..>2466
                     /locus_tag="PKO06_15005"
     CDS             1070..>2466
                     /locus_tag="PKO06_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71009.1"
                     /translation="MNTQKHFADRLILAVVVIGLCCWGSAVPCRAQTSPLISLLGVYG
                     DIPLNNTGKVDVMEDQAPPMDKIRNVRVAVMGPVELTDPSCVGGLYVPYADAKGMFHG
                     ATPHLSLAEKNEVLTWHNKVSYQSSWGDLVNAIIKLSTGACGGKDEDGNPQACAKHPP
                     AHGDHREGVDVMPSWADPGEFPTNQPHRYYWTLHLNAGNYLIQVDPSGINESPCADQH
                     NCQCPPDASGTKNCNGDPACKCYKADNGAVQLAPMGLVEGYQSGQKLEVIIHDRTPPH
                     IQPIDATATETDGGFPDLFKDPNGPKATTGDYCSVSELKVVDNASNKITTWFALNRKN
                     GPSDPSQWVFDSATDTVKNGDPLTHVFIPDDMYGDMEYSLFAWDNKNNLNPGDSKIDE
                     DQPSTCYGVEGESDLGANGTFQFATESTDLVPRLQSVGPNHHGLAFIDDNDRPNFYVK
                     IVSKRDREDPAKQDS"
BASE COUNT          558 a          643 c          702 g          563 t
ORIGIN      
        1 accaccatgt actcggcggt gtctctgctc gtgcgggccc gcgatgcgct caaccgtggc
       61 gaaatcgaga catcgcgcaa gttcgttgat gagatcctcg tgcgagagcc tcattacacc
      121 gaagcccagg atctcagaac ggaaatcgac aagcaggtgg agttgaagga actggtttca
      181 tcggtcgatg aacagatgtc gcgcggtcgg ctcgctgaag ccctggaatc ggcgaatgaa
      241 ctgataaaga agtatccggc gcaggctctg ttctatgttc ttcgggcccg cattcatctg
      301 aaaacccatc gatttcgcga cgcccagtct gacttgcgtg aagcgcggct gttgggatac
      361 gactttgcgc agattcggtc gtatctggtc gatgtgtatg ctggtcaggg tctgtttgcc
      421 caggcctatg cgctggcgca ggggaatgcc gagtgttccc cctgtcggcc gtgggacaca
      481 ctctggatct ggtattttaa gtcgtcgttc ttcggctgga tcattttctg tgcttcgttg
      541 gtcgtggctg gcggaacctt ttttgtgttc tggcgacagt tcgatgcgtt gattggccgc
      601 tactccctga gtcacttttt ttcggttgcg gcatgtctgt atcaatgctc cacgaacggc
      661 gcgacgtcgc agattgagcg actgatcagt atcacgaagc gcatgcctca tccctggtgg
      721 ctgtatgtta ccgccctgtc gttgatggaa atcggacaaa cagaccgatc tcaggagctc
      781 ctgctgcggg ctgcggcggc acccgagttg tcggcgaggg ctcatctcta tctgggtctg
      841 attcgcggcg aatccgaacc tcgactggcg gattacgatc ttgagcaggt ggtcatctgc
      901 tgtcagacag aagtggagca cccctggttg ccggatttcc tgcaaaccct ggaacagagt
      961 gttcttgacc ggtaccggga gcgtctgaaa aatcaccgat ttcttgaact ggcaatcgag
     1021 agttttcacg gtgtacaatc aacatgatgt gtcgagagag gagcattcca tgaatacaca
     1081 aaaacacttc gccgatcgtc tcattctggc tgtggtcgtc atcggcctct gctgctgggg
     1141 aagcgctgtc ccttgccgtg cacagacaag cccgctgatt tcacttctgg gcgtgtacgg
     1201 cgatattcct ttgaacaata ctggtaaagt cgacgtgatg gaagaccagg cgccaccgat
     1261 ggacaagatt cgcaatgttc gcgttgctgt catggggccg gttgaactga cggacccgag
     1321 ctgcgtcgga ggactgtatg ttccgtatgc cgatgccaaa ggaatgtttc atggggctac
     1381 tccgcatctg agtctggcgg aaaagaacga agtgctcacg tggcataaca aggtctctta
     1441 tcagagcagt tggggcgatc tggtgaatgc gatcatcaaa ctgtcgacag gggcttgtgg
     1501 tggaaaagac gaagatggga accctcaggc atgcgcgaaa cacccccctg ctcacggtga
     1561 tcatcgtgaa ggagttgatg tcatgcccag ttgggctgat ccaggggagt ttcccacgaa
     1621 ccagccgcac cggtattact ggacgctcca tctgaacgcc ggaaattatc tcattcaagt
     1681 cgatccttcc ggtatcaatg aatcaccgtg cgcagatcaa cacaattgcc aatgtccgcc
     1741 cgacgccagc ggaacaaaaa actgcaatgg tgaccccgca tgtaaatgct acaaggcaga
     1801 caatggcgcc gtgcaactcg cgccaatggg gttggtcgaa ggctatcaga gtggtcagaa
     1861 gctagaggtt atcatccatg atcgcactcc cccgcacatt cagccgatcg acgcgaccgc
     1921 aaccgaaacc gatggcggtt tccccgatct gttcaaggat ccgaatggtc cgaaggcgac
     1981 gactggcgat tactgctccg tcagcgaact caaggtcgtg gataatgcga gtaacaagat
     2041 cacgacctgg ttcgcgctca accgcaagaa tggaccgagc gatccgtcac agtgggtctt
     2101 tgattccgcg accgatacgg tgaagaatgg cgatccgttg acgcatgtat tcattcccga
     2161 tgacatgtat ggagacatgg aatactcgct gtttgcctgg gacaacaaga acaatctgaa
     2221 cccaggagac tcgaaaatcg atgaagacca accttccacg tgctacggag tggaaggtga
     2281 atcggacctt ggggcaaatg gcacatttca gtttgccaca gagtcaacgg atctggttcc
     2341 gcgtctgcag tccgttggcc cgaatcatca cggtctggcg ttcatcgatg acaatgaccg
     2401 accaaatttc tacgtcaaga ttgtctcgaa acgagatcgg gaagacccgg ccaagcagga
     2461 cagttg
//
LOCUS       DAORHD010000758         2464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51594_length_2464_cov_3.809465, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000758 DAORHD010000000
VERSION     DAORHD010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51594_length_2464_cov_3.809465"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKO06_15010"
     CDS             complement(<1..439)
                     /locus_tag="PKO06_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71010.1"
                     /translation="MKEWFRGIALFLCLGLVLVSAAAFADEDPSDYLVNIKPGFELSG
                     ETYGQFETPVYTVSPGLDNLLISVDANRSPEAIVRMYARFQTEKTSKWSRYAEFDGEF
                     HFAAVEPINAYQLLFVIRDQSKGKTVINQFTAQGRKLGEELMEF"
     gene            749..2302
                     /locus_tag="PKO06_15015"
     CDS             749..2302
                     /locus_tag="PKO06_15015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV71011.1"
                     /translation="MSLLLAAFMFFAGGAWAGERLINIKVAQYTQTIDFTTAQGTLWD
                     LGGKTGRLAAQQVAQIRGTLGQKAKKRYHVMVASVSLLEPQQLERTLTEWRSLGCEVY
                     TLESGQRFYAEDGKTLVFDNRHADVAVGVYEDLASAQAHVDRLAAQGKSSWIRTEIVS
                     LAKGNLVLTANGETLAEGESLMLVPESTILLKKVEYAVGYPWHGFADRTYLGTLTCRF
                     GAQDAIDCVLATPLEIVLAGVVPSEISAKAEIGALQAQACAARGEILAKIGVRHAGEG
                     FDTCSEQHCQVFNGESAVALEIAKKIAPTRGNVLTTSSGGLVDAVYAANCGGRGESNH
                     YVWTTQPNPILRGVWDISPEPNLDLTEEEQAGVFISNPPAAYCNNPAVEGGTNFRWKK
                     SLSTTEWKGIESSVGVGRIKTITDIARGLSGRMYRLTFVGERGNKTVMKELNIRKLFG
                     GLKSSCFVADWKKDAAGFIVSAELSGAGFGHGVGMCQTGAQALAKRGWSFDRILAHYF
                     PGSVLKKWY"
BASE COUNT          547 a          623 c          752 g          542 t
ORIGIN      
        1 tgaattccat cagttcttcg cccagcttgc ggccctgtgc ggtgaattga ttgatgaccg
       61 tcttgccctt gctctggtct cgaatcacga acagcagctg ataggcattg atcggttcaa
      121 cagccgcgaa atgaaactcg ccgtcgaact cggcataccg gctccatttg ctggtctttt
      181 ccgtttgaaa gcgcgcatac atgcgcacga tcgcctcggg tgagcggttg gcgtcgacgg
      241 agatcaacag gttgtcgagg ccgggagaaa cggtgtacac gggggtttca aactgtccat
      301 acgtttcgcc ggaaagctca aacccgggtt tgatgttgac cagataatca gaagggtctt
      361 cgtcggcaaa ggccgcggca gaaaccagaa cgaggcccag acaaagaaac agagcaattc
      421 cacggaacca ttccttcatg aacatactcc ttactgaaat actcttctgg cattatacaa
      481 cgcccgggca aaaagtcaaa aaaacagaaa gcgctcacag tcgcggaatt cttattgacg
      541 tgcacattac ggtgcccctg atgcaaaagt ttcatccgat taagagaaaa aattgtccga
      601 ttcggggggt tatggacccc tgggtatgtc tgataaaatg taccaataca ctccgggtat
      661 ccggtcattt gttcattcgg cacatcagga ggaaggacag atggttctgg ccagaaacct
      721 tatgaaaacg ttgaggcagg ggaaagtgat gtctctgctt cttgctgcgt tcatgttctt
      781 tgccggcggt gcgtgggctg gtgagcgtct gatcaacatc aaggtggcgc agtatacgca
      841 gacgatcgat tttacgacgg cgcagggcac cctctgggat ctcggcggca agaccggtcg
      901 actggcagcg caacaggtgg cgcagattcg cggaacgctc gggcaaaagg cgaagaaacg
      961 gtatcatgtc atggtcgcgt cggtttctct gctcgaacca caacagcttg agcgaacgct
     1021 gacggagtgg cgcagtcttg gatgcgaagt gtacacgctc gaatccggtc agcggttcta
     1081 tgccgaagac ggcaagacac tggtgttcga caatcgccat gccgatgtcg cggtaggcgt
     1141 gtatgaagat ctggcttcgg cgcaggcgca tgtcgacaga ctggcggctc agggaaaatc
     1201 aagctggatt cgcacggaga tcgtgagtct cgccaaggga aacctggtgc tgaccgcgaa
     1261 tggtgagacc ctggcggaag gcgagtcgct gatgctggtc cctgagtcga cgattctgct
     1321 gaagaaggtt gagtatgccg tgggataccc gtggcatggt tttgctgatc gcacgtatct
     1381 cggaacgctc acgtgccgat ttggcgctca ggatgcgatc gactgcgtgc tggcgacccc
     1441 cctcgaaatc gttctcgccg gagtggttcc ctcggagatt tcggcaaagg ccgagattgg
     1501 cgctctgcag gctcaggcgt gcgccgcccg cggcgagatt ctcgccaaga ttggggtgcg
     1561 tcatgccgga gaaggctttg acacctgctc tgaacagcat tgtcaggtgt tcaacggaga
     1621 gtcggccgtg gctctcgaga tcgcgaagaa gattgctccg acccgtggca acgtgctgac
     1681 cacgtcttcc ggcggtcttg tcgacgcggt gtacgcggcg aactgcggcg gtcgcggcga
     1741 atcgaaccac tatgtctgga ccacgcaacc gaacccgatt ctgcgcggtg tctgggacat
     1801 cagccctgaa ccaaaccttg atctgactga agaggaacag gcgggcgtgt tcatcagtaa
     1861 cccgccggcg gcctactgca acaatcctgc ggtcgaaggc ggaacgaact tccgttggaa
     1921 gaagtccctg tcgacgactg agtggaaggg cattgagagc tccgttgggg ttggtcgaat
     1981 caagacgatc accgacattg cccgtggatt gtcgggacga atgtaccggc tgacgttcgt
     2041 tggtgaacgt ggaaacaaga ccgttatgaa ggaattgaat attcgaaaac tgttcggggg
     2101 tctgaaaagc tcctgttttg ttgccgactg gaaaaaagat gccgccgggt ttatcgtctc
     2161 ggctgaattg tcaggcgcag gattcggaca cggcgtcggt atgtgccaga ccggcgcgca
     2221 ggcgctggcg aagaggggtt ggagtttcga tcgcattctc gcgcactact tcccggggag
     2281 cgttttaaag aaatggtact gagcttgacc ggtttcacgc aggggttgct tcatcgagca
     2341 tgaagtgcca gcattgcggc cgtgtcagct gtctcgacgt tctgtatgag aaatgccgtt
     2401 tctgtggtca gctccattgc cttgatgccc agtcacagta tgagatcaat ctgttgctcg
     2461 ggcg
//
LOCUS       DAORHD010000759         2463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51629_length_2463_cov_2.975914, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000759 DAORHD010000000
VERSION     DAORHD010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51629_length_2463_cov_2.975914"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1743
                     /locus_tag="PKO06_15020"
     CDS             <1..1743
                     /locus_tag="PKO06_15020"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV71012.1"
                     /translation="RADLVTMPVNAIHEWKNIQRRSAIWEADGRESSVALTEANGLAF
                     SINGKCDGNSIEDAPTQIMLGMVSALHHPSPKSAFVVGLGTGCSAGWLGAITDMERVV
                     VAELEPMVLEVASRCAPINHAVLANPKVQVRLGDARELLLIDNQTYDLIVSEPSNPFR
                     AGISSLFTREFYEAVNRRLAQGGLFSQWVQGYEIDVQTMKTVYATLRTVFPYVETWQT
                     SYGDYLLICSRERLAYSRTLLEQRLKQEPWKGALWQGWRVNSVEGIFSHYVANADFVN
                     MVHNTTNPRDHLSTDDRVTVEFGLLRTMGRKNLGMMQALQVEANSNQLQKPLMRDGTI
                     DWAEVRDQNLLALTLSNQECKTLDDPDRGFLLRVAAHTNYRRENFRGFVQNWSGQDRK
                     PLHPGESLMLAESFARFGAPETTAMVEPAQAEFPGESQLILATYFSEIGSYSEAVACF
                     EKGFGIFRQRPFPVEPIIRRGMEMAMNLALKHPELAPRLAESLRQPFLLSAVNELRVQ
                     AIVGLLPKLEPVLVREYCHALEPNVPWQDEFLITRRDCYKATNDPLWQQAAADVDAYL
                     AGEPNRGGTDAFLK"
     gene            1806..>2463
                     /locus_tag="PKO06_15025"
     CDS             1806..>2463
                     /locus_tag="PKO06_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71013.1"
                     /translation="MNGFSGESKPLYRQLLDFLGLRRSMVGILAMCILIGLGEKMAER
                     FLPLYLVALGGGAWAVGLLNSLDNLLSALYSFPGGWMADRFGPRRSLLAFNLVAIAGY
                     LLVILVPSIWAVFVGSFLFISWTSLSLPATMGLISVVLPKEKRTMGVSLHSLIRRIPM
                     ALGPILGGFLVTQYGTETGVRLAFIGAIGMAVLAILFQEYMITDVSEKKDNAATSESE
                     S"
BASE COUNT          491 a          744 c          707 g          521 t
ORIGIN      
        1 cgcgccgatc tcgtcacgat gcctgtgaac gccatccacg agtggaaaaa cattcagcgc
       61 cgcagtgcca tctgggaagc cgacgggcgc gagagcagcg tggccctcac cgaagcgaac
      121 ggtctggcat tcagcatcaa cggcaagtgc gacggcaact cgatcgaaga cgcgccaacc
      181 cagatcatgc tgggtatggt cagtgcgctc catcatccat cgccgaaatc ggcttttgtg
      241 gtcggactcg gaaccggttg ttcagccggg tggctagggg ccattacgga catggagcga
      301 gtcgtggtcg cggaactcga acccatggta ctcgaagtcg ccagtcgctg cgcaccgatc
      361 aaccatgcgg ttctggccaa cccgaaagtc caggtcagac ttggcgatgc aagagaactg
      421 ctgctgatcg acaaccagac ttacgatctg atcgtctctg aaccttcgaa cccctttcgc
      481 gccggaattt ccagtctctt cacccgtgag ttttacgaag ccgtcaatcg cagactggca
      541 caaggtgggt tgttttctca atgggtacaa ggatacgaga tcgacgttca gacgatgaag
      601 accgtgtatg caacgctgag aaccgttttc ccctacgtcg agacctggca gaccagctac
      661 ggtgactatc tgctgatctg ctcccgcgag cgcctggcct actcacgcac gttgctcgag
      721 cagagactga agcaggaacc ctggaaggga gctctctggc agggctggcg cgtcaacagc
      781 gtcgaaggca tcttctctca ctacgtggcc aacgccgatt tcgtgaatat ggtacacaat
      841 accaccaatc cgcgcgacca cttgagcacc gacgaccggg taaccgtcga gttcggtctg
      901 ctgcgcacga tggggcgaaa gaaccttggc atgatgcagg cgctgcaggt tgaagccaac
      961 tccaatcaac tgcagaaacc cctgatgcgc gacggaacca ttgactgggc tgaagtgcga
     1021 gaccagaatc tgctggctct caccctgtca aaccaggagt gcaagacgct cgatgatccg
     1081 gaccgcggat tcctgctgcg cgtcgccgct cacaccaact atcgccgcga aaactttcgc
     1141 ggtttcgtac agaactggag cggtcaggac cgcaaaccac ttcaccccgg ggaatctctg
     1201 atgctggccg aatcatttgc acgatttggc gctcctgaga ccaccgccat ggtcgagcct
     1261 gcccaggccg agtttcccgg agaatcacag ttgattctgg cgacctattt cagcgagatc
     1321 ggcagctatt ctgaagccgt cgcctgtttc gagaaggggt tcggcatctt tcgccagcga
     1381 ccgttcccag tagaacccat cattcgccgc ggcatggaaa tggcgatgaa tctggcgctg
     1441 aagcatccgg aactcgcccc gcgcctggcg gaaagtctgc gacaaccttt cctgctttcc
     1501 gcagtcaacg aactccgcgt tcaggcgatc gtcggcctgt tgcccaaact cgagccagtc
     1561 ctggttcgtg aatactgtca cgcccttgag cccaacgttc catggcagga cgaatttctg
     1621 atcacgcgtc gcgactgcta caaagccacc aatgatccgc tttggcagca ggcggctgcc
     1681 gacgttgacg cctatctcgc cggcgagccc aaccgcggcg gcaccgatgc ctttctgaag
     1741 tagtcctcgc ttaccagaga tcggttcagc gtcagcggat tcggttgtga taccctgagg
     1801 gtgccatgaa cggattttca ggcgaatcga aacccctcta ccgccaactg ttagactttc
     1861 tcgggttgcg gcggagcatg gtcggtatac tggccatgtg cattctcatc ggcctcgggg
     1921 aaaaaatggc cgagaggttc cttcctctgt atctggtggc cctgggcggc ggggcctggg
     1981 ccgtcggcct cctgaactct ctggataacc tgctttctgc cctgtactcg ttcccgggcg
     2041 gatggatggc ggatcggttc ggtccccggc gctccctgct ggcgttcaat ctggtggcga
     2101 ttgccgggta cctgctggtg atcctggtcc cctccatctg ggccgtgttc gtggggtcct
     2161 tcctcttcat ctcctggacg tcgctttccc ttccggcaac gatgggtctc atttccgtcg
     2221 ttctgccgaa ggaaaaacgc acgatgggcg tctcgctgca ctccctgatt cgccggattc
     2281 ccatggccct ggggccgatc ctgggcggat ttctggtgac gcagtatggg acggaaaccg
     2341 gagtgcgtct cgcctttatc ggagccatcg gcatggcggt gctggccatt ctgttccagg
     2401 aatacatgat cacggatgtg tcggaaaaga aggataacgc tgccacgtcc gagtcggagt
     2461 cag
//
LOCUS       DAORHD010000760         2460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51733_length_2460_cov_2.539709, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000760 DAORHD010000000
VERSION     DAORHD010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51733_length_2460_cov_2.539709"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PKO06_15030"
     CDS             <1..1052
                     /locus_tag="PKO06_15030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV71014.1"
                     /translation="VPDDKRGVMTGTPGEIAVPPPGLQEIQSPVGAIGNRFCWEVMHC
                     DNLRCPVRQRQIIRCFGFYGSRPAEEKQRLCGCQRSCDECHYKRGWDLGMLHEGLFED
                     LLEERRRKMARVNSLQQRGIVEIYLQELARKPLTRSEEMTLAKRLAGDRKASELLLTA
                     NLKLVVRISSQFTNRGLSLMDLIQEGNLGLIKAVSKFDWRLGYKFSTYAAYWIRHYMQ
                     RAVSNQGRVLRVPHHLLMVAHKIKRAISEFQGQWQRAPNLTELAGLVGLEEDKVLDII
                     RITETPISIEARQPDGDDEESSLEYFLADKNRLTPKRRCSRKPRRKPARPHLKHCPTA
                     CAKWSNASTAFVKRK"
     gene            944..1180
                     /locus_tag="PKO06_15035"
     CDS             944..1180
                     /locus_tag="PKO06_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNV71015.1"
                     /translation="MLEKAKAEACAAALKTLPDRLREVVECFYGFREEEVSLAELGRR
                     MGVSRERVRQLLQEALKKLGEQEFVANLKDYLTP"
     gene            complement(1328..1768)
                     /locus_tag="PKO06_15040"
     CDS             complement(1328..1768)
                     /locus_tag="PKO06_15040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71016.1"
                     /translation="MEHIGPRQFAQSITIKPRLENIPQVQEFIQGILADAKASTEFRK
                     QIKLVVDESVANAVDHGAGNSGVGEIKVSCQIIDDSFQLMVEDYHGRLFDPEYFRRIA
                     MVRDWGKGGRGIMLIEGYMDHVSYVIDEGRHTILYMEKQFKAAD"
     gene            complement(1768..>2460)
                     /locus_tag="PKO06_15045"
     CDS             complement(1768..>2460)
                     /locus_tag="PKO06_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71017.1"
                     /translation="IELRDITQTRDKSRRDLEIASFIIHRFPELTEKMTELSKDLSRE
                     NPSTSEFLETLREVNRQVNKMMAFTELEAGPLRIQRMPINLEELITEVCEMHAELVQE
                     RKITVKTSFSFGDESVEADDDWLKKLLSILLENAIMYTEEGAEVLIQTEKTSEGRFRC
                     RIQNPTDFTLDVEQAHRYFDLRTQLEEFESFQATDFSLELPLARHIVEAHSGVIFIEP
                     DRENLFSVVFDI"
BASE COUNT          535 a          642 c          733 g          550 t
ORIGIN      
        1 ccgtgccgga cgacaaacgt ggcgtcatga ccggaacgcc gggggaaatc gcggtgccgc
       61 cgccggggct gcaggagatc cagagcccgg ttggtgcgat cggcaaccgc ttttgctggg
      121 aagtcatgca ttgcgacaat ctgcgctgcc cggtgcggca gcggcagatc attcggtgct
      181 tcggtttcta cggttctcgc ccggcagaag aaaaacaacg tctgtgcggc tgtcagcgtt
      241 cctgcgatga gtgccactat aaacggggct gggatctcgg tatgctgcat gaagggttgt
      301 ttgaagacct gctcgaggaa cgtcgtcgta aaatggcgcg tgtcaacagc ctgcagcagc
      361 gaggtattgt cgagatctat ctgcaggagc tggcacgtaa gccgcttaca cgctcagaag
      421 agatgactct cgcgaaacgt ctcgcgggtg accgcaaggc ttctgagttg ctgctcacgg
      481 cgaacctgaa actggtggtt cgcatcagca gtcagtttac caatcgcggg ctgagtctga
      541 tggaccttat tcaggaaggc aatctcggac tgatcaaagc cgtgtcgaag ttcgactgga
      601 ggctgggata caagttttcc acctatgccg cctactggat tcgtcactac atgcagcgtg
      661 ccgtgagcaa ccagggccgg gttctgcgtg tgccgcatca tctgctgatg gtcgcacaca
      721 agatcaaacg cgcgatcagc gagtttcagg ggcagtggca gcgggccccg aacctgactg
      781 agcttgccgg gttggtcggg ctggaagagg acaaggtgct cgacatcatt cgcattaccg
      841 agaccccgat ttcgatcgag gcacggcaac ctgacggtga cgatgaagaa agttcgctgg
      901 aatactttct cgccgacaag aaccgcctga ccccgaagag gagatgctcg agaaagccaa
      961 ggcggaagcc tgcgcggccg cacttaaaac actgcccgac cgcctgcgcg aagtggtcga
     1021 atgcttctac ggctttcgtg aagaggaagt gagtctggcc gaactcggac gacggatggg
     1081 tgtcagtcgc gagcgggtgc gtcagttgct gcaagaggct ctgaaaaaac tgggcgaaca
     1141 ggaattcgtc gccaacctga aagattacct tactccctga cgctttctcg tcaatggaaa
     1201 agcgatcaca gaactcggac aaatggagtt tgcatgctgt cggtcagtcg cggcgtataa
     1261 agggaaccgc tcgtggcgat caggaaagag tccaggctgt tcggtgcaaa gacttgttct
     1321 ctgagcacta atcagcggct ttgaactgct tttccatata caggatggtg tgtcgcccct
     1381 cgtcgatcac atagctgacg tgatccatgt agccttcgat cagcatgatg ccgcggcctc
     1441 ccttgcccca gtcgcgcacc atggcgatgc ggcgaaagta ttccgggtcg aacagccgac
     1501 catgataatc ctcgaccatg agctgaaatg agtcgtcgat gatctgacaa ctcaccttga
     1561 tttctcccac gccggagttc cccgcgccgt gatcgacggc attggcaacg gactcgtcaa
     1621 cgacgagttt gatctgcttg cgaaactcgg tggaagcttt cgcgtcggcg agaataccct
     1681 gaatgaattc ctgcacctgc ggaatgtttt ccaggcgtgg tttgatggta atagactggg
     1741 cgaactgacg tggtccgatg tgttccatta gatgtcaaac accaccgaaa agagattttc
     1801 gcgatcgggt tcgatgaaga tcacgccgct atgagcttcg acgatgtggc gtgcgagagg
     1861 cagttcgaga ctgaaatccg tggcctgaaa actctcgaat tcctcgagtt gggtgcgcag
     1921 atcgaagtag cggtgcgcct gctcgacatc cagagtgaaa tcagtcggat tctggattcg
     1981 gcatcggaac cggccttcag acgttttctc ggtctgaatc aggacttctg caccttcttc
     2041 ggtatacatg atggcgtttt cgagcagaat gctgagcagc tttttcagcc aatcgtcgtc
     2101 ggcttcaacg ctctcgtcgc caaagctgaa ggaagtcttg accgtgatct tgcgctcttg
     2161 caccagttcg gcgtgcatct cgcagacttc agtgatcaac tcctcgaggt tgatgggcat
     2221 gcgttgaatg cgcagagggc ccgcttcgag ctcggtaaag gccatcatct tgttgacctg
     2281 gcggttgact tctcgcagcg tttcgagaaa ctcgctggtc gaggggttct cacgcgagag
     2341 atctttggaa agctcggtca tcttttcggt cagttcggga aaacgatgga tgatgaagct
     2401 ggcaatttcc agatcgcggc ggcttttgtc gcgagtctgc gtaatgtcgc gcaactcgat
//
LOCUS       DAORHD010000761         2457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_51818_length_2457_cov_3.853872, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000761 DAORHD010000000
VERSION     DAORHD010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51818_length_2457_cov_3.853872"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..429)
                     /locus_tag="PKO06_15050"
     CDS             complement(136..429)
                     /locus_tag="PKO06_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71018.1"
                     /translation="MKTFTHTWEGEIFLLHLELNRSDAVSYQADLVRAFRDGKTSSEV
                     IRVKEQETSYRFLVTRTHPRETSFTFQYPDDVNLKAFKLDDRAYYPTESFFEG"
     gene            complement(435..>1286)
                     /locus_tag="PKO06_15055"
     CDS             complement(435..>1286)
                     /locus_tag="PKO06_15055"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71019.1"
                     /translation="ITIERVEAEAVFHYKPGAKILSLGNFGCNLDCDYCQNWVYSQFQ
                     FTPPESIEEYTCDEVIAKAREDGIEILSWTYNDPAVWFEFVIDTAREARKAGLKNLFK
                     SAFFLTREAVEKLCEVIDVFAISIKAMDEDYYTKFTKGWLEPVLDNARLVHSKGIPYE
                     VSNLVVTGLTNNLPSYDLMIDFLLRDLSPKTPIHFTRFHPDYKYMHVEKTPIDDVMAA
                     RKRAQERGIIYPYIGNAFESEGLNTYCPSCKTLLVERFGLSTYIQDSLTADSKCAKCG
                     FQTPIIR"
     assembly_gap    1287..1296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1297..1521)
                     /locus_tag="PKO06_15060"
     CDS             complement(<1297..1521)
                     /locus_tag="PKO06_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71020.1"
                     /translation="MSNVYTADAKRHNLHVAWNDTRKEAYLYDKLADGKVRCGVCPRR
                     CQIAPGQVGFCKVRKNIDGTLYTQNYGKATH"
     gene            1700..>2457
                     /locus_tag="PKO06_15065"
     CDS             1700..>2457
                     /locus_tag="PKO06_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNV71021.1"
                     /translation="MMKNEQERAVLSGNAAIARGAWEAGVRFASAYPGTPSTEILEYC
                     GTYDEIDAQWGVNEKTAMETVIGASYGGARALCAQKHVGLNVAADPFFTVAYTGVNAG
                     LVMVTADDPGMHSSQNEQDNRHFARMAKIPMFEPSSSQDAKDMTAAAYEMSEKYDTPV
                     LLRTTTRISHSEGVVALGPRQQAPDRIFQRNMEKYCVLPQNARKLHVKVEQRLQALEA
                     YGCDCPWNRIEDGDRAIGIIASGISYAYAREVFP"
BASE COUNT          512 a          633 c          768 g          534 t
ORIGIN      
        1 ggtgaagcgg gatcattcaa gaaaatcaac cgtgaccatt cgggaacgaa gacggcatac
       61 aacccgttga aggacaacag aagcccttcg ccgaggcgct gacggctcat gctgatcatg
      121 cggccttctc cgcggtcatc cctcgaaaaa cgattcggtg gggtagtacg cgcggtcgtc
      181 gagtttgaag gctttcaagt tcacgtcgtc tggatactga aacgtgaagc tggtttcacg
      241 cggatgcgtg cgggtgacca gaaatcggta actggtttcc tgttccttga cgcgaatgac
      301 ttcgctgctg gtttttccgt cgcggaacgc gcgaaccaga tcggcctgat acgaaacggc
      361 gtcgctgcga ttcagttcaa gatgcagcag gaagatttcc ccttcccagg tgtgcgtgaa
      421 ggttttcatg ggcgtcagcg aatgatgggg gtctggaacc cgcacttcgc gcatttggag
      481 tcggcggtca gagaatcctg aatgtaggtc gagagcccga accgctcgac cagcagggtc
      541 ttgcaggagg ggcaataggt attgagccct tctgactcga aggcgttccc gatataggga
      601 tagatgattc cgcgttcctg cgcgcgtttc cgggcggcca tgacgtcatc gatcggggtc
      661 ttctcgacgt gcatgtactt gtagtcgggg tggaagcggg tgaagtgaat cggggtcttg
      721 ggagacaggt cgcgcaggag gaagtcgatc atcagatcgt aactgggcag gttattggtg
      781 aggcctgtca cgaccagatt actgacttcg tagggaatgc ctttcgagtg tacgagtctg
      841 gcgttgtcta aaaccggttc gagccagcct ttggtgaact tcgtatagta gtcctcgtcc
      901 atggctttga ttgagatggc aaaaacatcg atcacttcac acagcttttc gacggcttcg
      961 cgggtcagga aaaacgcgga tttgaagaga ttcttcaaac ctgccttgcg ggcttcgcgg
     1021 gccgtgtcga tgacaaactc gaaccagacg gccgggtcgt tgtaggtcca gctcaggatc
     1081 tcgatgccat cttcgcgggc tttggcaatc acttcgtcac aggtatattc ttcgatcgat
     1141 tccggggggg taaactgaaa ctggctgtag acccagttct gacagtagtc acagtcgagg
     1201 ttgcagccga agttgccgag agagagaatt ttcgcgcccg gtttgtagtg aaagactgct
     1261 tcggcctcga cgcgctcgat ggtgatnnnn nnnnnnatga gtcgccttgc cgtaattctg
     1321 ggtatacagt gttccgtcaa tgttcttgcg caccttgcag aagccgacct gacccggggc
     1381 gatctggcaa cgccgggggc agacgccgca gcgaactttg ccgtcagcca gtttatcgta
     1441 gagatatgct tccttgcggg tgtcgttcca ggcgacgtgg aggttgtgtc gcttggcgtc
     1501 ggccgtatag acattgctca tgagaaaaac ctctcttcct cggctgtgcg tcgagggcgc
     1561 tcagtataaa cccgcgctcc cggcctgtca acgaaagttg ccggaatctc gtgaaacggg
     1621 ggagtgagac gaggttcctg tggtataatc ctgccgctcg cgtggcgaga gacagatgag
     1681 ttgcagagca aggagcggaa tgatgaagaa cgaacaggaa cgggccgtgc tctcgggaaa
     1741 tgccgcgatt gcccgcggag cctgggaagc cggtgtgcgc tttgcctcgg cgtacccggg
     1801 aaccccctcg accgagattc tcgagtattg cggcacctac gacgagatcg acgctcaatg
     1861 gggcgtgaac gagaagactg ccatggaaac ggtcatcgga gccagttatg gtggagcgcg
     1921 ggcgttgtgt gcccagaaac acgtcggctt gaacgtggcg gctgaccctt ttttcacggt
     1981 tgcctatacc ggcgtgaacg ccgggttggt catggtgacc gccgatgatc ccggcatgca
     2041 cagctcgcag aacgaacagg acaaccggca ttttgcgcgc atggcgaaga ttccgatgtt
     2101 tgaaccgagc tcgagtcagg atgccaagga catgacggcc gccgcctatg agatgtccga
     2161 gaagtacgac acgccggtgc tgctgcgtac caccacccgc atttcgcact ccgagggagt
     2221 ggtcgcgctg gggccgcgtc aacaagcccc ggatcgaatc tttcagcgca atatggagaa
     2281 atactgcgtc ctgccgcaga atgcgcgcaa gctgcatgtg aaggtcgaac agcgcctgca
     2341 ggcactcgag gcctatggct gcgactgtcc ctggaatcgc attgaagacg gtgatcgggc
     2401 gatcggcatc atcgcttcgg gcatctcgta tgcctatgcg cgcgaggtgt ttcccca
//
LOCUS       DAORHD010000762         2448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52117_length_2448_cov_3.290430, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000762 DAORHD010000000
VERSION     DAORHD010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52117_length_2448_cov_3.290430"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKO06_15070"
     CDS             complement(<1..954)
                     /locus_tag="PKO06_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA phosphorothioation-associated putative
                     methyltransferase"
                     /protein_id="HNV71022.1"
                     /translation="MEFATYLGYVRQIPFGKRLPEALYIHCCGFEELVPELHRFIMGQ
                     MRKLQLEAFPFDLVKLHLREFKISLLSYPDFWDDPYPALKMSCTIDLLSHRTKLSSYE
                     HSENPPILHRKETFLPQGHPKIAEFSAQTKTAEEAGLFENAQTIGFKQNWLNRIKKLG
                     YVLNGATLEKTQKTRTKNSSNQPGSALAENLSIEDLTNVDIKRHLTAIERNILSGPMQ
                     SLYNHGFLDGQHSVFDFGCGKGDDLRELQAHGVPAKGWDPVYQPDQPKEPADVVNLGF
                     VLNVIEKPAERRAVLSEAFSLAKKVLSVSVMLGGESTISKFK"
     gene            1165..1509
                     /locus_tag="PKO06_15075"
     CDS             1165..1509
                     /locus_tag="PKO06_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71023.1"
                     /translation="MSECKCMNPPFEGDVFRWQEVGTDMTFARYGEVKLATCNTCGSL
                     WVRYFIEDEAFSRSGRWFMGQISKETADTLTPENAVDIINSLEWHYCGGSYYSSAGFR
                     STHKPIKVDLFG"
     gene            1556..1753
                     /locus_tag="PKO06_15080"
     CDS             1556..1753
                     /locus_tag="PKO06_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71024.1"
                     /translation="MTTRATSFVQGTRQVVAVADLHGHLSALKRLLKSVEAKIGSDYF
                     LVSPNIRSLRRRKAHYPRLGR"
     gene            complement(1915..2385)
                     /locus_tag="PKO06_15085"
     CDS             complement(1915..2385)
                     /locus_tag="PKO06_15085"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV71025.1"
                     /translation="MNANEYFDLKLFRTNFRARDKRDALSQMARVIHKAGYCETEAEA
                     LSVLWSRELIGSTGLTGGLAVPGDIGTQMYRRSGIGILVAPDGVPFDTQDALPVRVFV
                     LLALSKPVTGMWLKCFAIVSRCLRRRIFRSDLFASSTPEEVLRVICKYGDFELE"
BASE COUNT          591 a          645 c          626 g          586 t
ORIGIN      
        1 tttgaactta gagatcgtgc tttctccccc aagcatgact gataccgaaa gaactttttt
       61 agcgagggaa aacgcctcag acaagacggc tcggcgctcg gcaggttttt caatgacgtt
      121 gagcacaaac ccaagattca ccacatcggc cggttccttc ggctgatccg gctggtagac
      181 aggatcccag cctttcgccg gcaccccatg cgcctgcaac tctctcaaat catctccctt
      241 gccacagcca aaatcgaaaa ccgagtgctg cccgtcaaga aatccatgat tgtacagact
      301 ctgcataggg cccgacagaa tgtttcgttc aatcgcggtt aaatgacgct tgatgtcaac
      361 atttgtaaga tcttctatgg aaagattttc agcaagcgct gaaccaggct gattacttga
      421 gttctttgtc cttgtttttt gagttttttc aagtgtcgcg ccattcaaga catagcccaa
      481 cttcttgatc cgattgagcc aattctgttt gaatccgatc gtttgcgcgt tctcaaacaa
      541 cccggcttct tcagctgttt tcgtctgtgc cgagaactcg gcaatctttg ggtgaccctg
      601 cggaagaaaa gtttccttgc gatgaagaat cggcggattt tccgaatgct cgtatgaact
      661 cagcttcgtt cgatgcgaca agaggtcaat tgtacaactc atcttaagcg ccgggtaagg
      721 atcatcccag aagtctgggt atgagagaag agaaatctta aactctcgca gatgcagttt
      781 tacaagatca aatgggaagg cttctaactg cagtttcctc atctgcccca tgatgaaccg
      841 gtgcaattcg gggaccagtt cttcgaagcc gcaacaatga atgtacagtg cttctggtaa
      901 acgtttccca aagggaatct gtcttacgta acccagatac gtagcgaatt ccatacacca
      961 catccgatta cactaaaaat ccgtaaggcg ttgagcgaca tcttcatttc ccacgcctat
     1021 acgcccccag ccatatacca tatcgaataa ctcgatctaa tccaagagat tgttgcgttt
     1081 ttccggaatg ttttgggtga acgaggatgg ctgagaagtg agtatcagca gaagcaggaa
     1141 cattccaaca aggagggcat gacgatgtct gaatgcaagt gcatgaatcc gccgtttgag
     1201 ggagatgttt ttcggtggca ggaggttggc acggacatga ccttcgcgcg atacggtgag
     1261 gtcaaactgg cgacgtgcaa cacctgcggg agcctttggg tgcgatactt tattgaagat
     1321 gaagcattct ctcgctctgg tcgatggttc atgggccaga tttcgaaaga gactgccgat
     1381 accctgaccc ctgaaaatgc cgtcgatatc atcaattcgc tggagtggca ctattgcgga
     1441 ggatcgtatt actcttcggc gggctttcga agcactcaca agcccattaa agtagacctg
     1501 ttcgggtgaa cacttcatct tgtcgctgga acgaacatct caccaggagg gaaccatgac
     1561 gactcgggca acgtcgtttg tgcagggaac aagacaggtg gtagcggtcg cggatctgca
     1621 cgggcatctt tcggcgttaa agcggcttct gaagagtgtc gaagcgaaga tcggctcgga
     1681 ctattttctc gtctcaccca acataaggtc acttcgtcgg cgaaaagcgc attaccctcg
     1741 actcgggcgc tgacagggga ggaaacctgt gggcggtgat ccttccttac aggcagtttg
     1801 tcagcgtccc agttctcagg tagtcacagc ccctattcaa gagcctcacg cggctcttca
     1861 ctttcgacca tctcgcgggc tatctggagg cccgagagat ggtcgatggt ttttttattc
     1921 aagttcaaag tcgccgtact tgcaaataac gcgcagaacc tcttctggag ttgacgacgc
     1981 gaacaggtcg cttcgaaaga tgcgacgccg caggcaccgc gagacgatgg cgaaacactt
     2041 cagccacatg cccgtgactg gcttgctgag cgcgagaaga acgaacactc gcacaggaag
     2101 agcgtcctgc gtgtcgaagg gaaccccatc tggagcgacg agaatgccga tgcctgagcg
     2161 acgatacatc tgggttccga tgtcgccagg gaccgcgagg cctccggtga ggcccgtcga
     2221 gccgatcagc tcacgactcc agagcaccga cagcgcttcc gcttccgtct cgcagtagcc
     2281 cgccttgtgg atcacgcgcg ccatctggga cagggcatct cgtttgtctc tggcccgaaa
     2341 gttggtgcga aacagcttca gatcgaagta ttcgttcgcg ttcatcggtc cgactgtcat
     2401 cgaacacgct atattccccc agtatggtca tgaaaattcc ccccacct
//
LOCUS       DAORHD010000763         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52209_length_2445_cov_3.737657, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000763 DAORHD010000000
VERSION     DAORHD010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52209_length_2445_cov_3.737657"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKO06_15090"
     CDS             <1..326
                     /locus_tag="PKO06_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71026.1"
                     /translation="GVKKKSKHSTWWEYATIIAIDDGTHFPVSDYVREDLLQANTKAR
                     LLADVIGCHYQAGQLECEARVIRDQVTGKVRIEQRPRSILDELLPVLLVLVGFGAVMF
                     FASLF"
     gene            complement(333..521)
                     /locus_tag="PKO06_15095"
     CDS             complement(333..521)
                     /locus_tag="PKO06_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71027.1"
                     /translation="MRLSREQAYYILTNLDTFVTQFESATQKKVTPGALQIIHEILTD
                     ISFPAAVAEQTRVQDTLH"
     gene            636..1775
                     /locus_tag="PKO06_15100"
     CDS             636..1775
                     /locus_tag="PKO06_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71028.1"
                     /translation="MTQAPGSYLIGAVEQAVAGGYDPAFPPISVSLNGALAKWRKSIQ
                     ILDLRVNDPGLQRYQGAIEQTLTQLRNLSIDCILTLPEHVPEFFTLPPEVLPPPSGPE
                     SNSRRIFATRKEQGVATTSVARFQRCFLAVPHGYSSAKTARALALEDLTPILDLAQKH
                     GLKRVIVPVSDPGLFLDAQSQRVFQQKFVQFHLEAEKRGIKLLVKNGGLSKDLFLKLR
                     KETGCKLAFNLGTAFFERLDLLESLREQQEYIGVLFLHQALSGIDVLNEWTDRVRKTL
                     NQTIDAVAAYQNPENQKDPADTEYYLSQLRKAFFSFRDARQNSTTHLGIFQHGAINFV
                     PVLKYLKQLIEQGSDLTVFFEAVPNLKNLEFFERYVVSDILPAPI"
     gene            1798..>2445
                     /locus_tag="PKO06_15105"
     CDS             1798..>2445
                     /locus_tag="PKO06_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71029.1"
                     /translation="MTEKRRTGFARLLPLLLFTICWFPAVPAGAASPLRNAVATDEPA
                     ISRPYTLQETLRRAEELFLQNNYREALVFYYEGLDQVEANEIRGKLHFRIGECLEGIR
                     RFDFAAYHYKLAVKANLGRGLTERAVNKLKLLPKLAQKEEAMRLYQRAMAAYRKRNVR
                     DAIDDYLKSLRLEPSLMAQDNSGLLDDAIQYLMLLSESKDQEPSRLLKLASLLELR"
BASE COUNT          505 a          732 c          680 g          528 t
ORIGIN      
        1 acggggtgaa gaaaaaatcg aagcacagca cgtggtggga gtatgccacg atcatcgcca
       61 tcgacgacgg cacccatttt ccggtgtcag actacgtgcg cgaagatctg ttgcaggcaa
      121 acaccaaggc ccgcctgctg gccgacgtca tcggctgtca ttaccaggcg ggtcagttgg
      181 aatgcgaggc gcgggtgatt cgcgatcagg tgaccgggaa ggtgcgcatc gagcagcggc
      241 cccgttcgat tctggacgaa ctgttgccgg ttcttctggt actggtcggc tttggtgcgg
      301 tgatgttttt cgccagtttg ttctaagaac ggtcagtgca gagtatcctg caccctggtc
      361 tgttcagcga ccgccgccgg gaacgaaatg tccgtcagaa tctcatggat gatctgcagc
      421 gccccgggag tcactttttt ctgtgtcgct gactcaaact gagtcacaaa ggtatcgaga
      481 ttggtcagaa tgtagtaggc ctgttcgcga ctgagtctca tggattcgtt ccctccgccc
      541 ttgatgaagt ggtttttcta tcccaaacta tagatcgtca ctttgccggg ttttccttag
      601 cccctgcggg aaaaatgtga taccattggc ccgttatgac gcaagcacca ggttcctacc
      661 tcatcggcgc agtagaacag gcagtagccg gcggttacga tccggcattt cctcccattt
      721 ccgtcagcct gaacggggct ctggcaaaat ggcgcaagag cattcagatt ctcgatttgc
      781 gcgtcaacga cccgggtctg cagcgttatc agggcgcgat cgagcagacc ctgacgcaac
      841 tgcgcaacct gtcgatcgat tgtattctca cgctccccga gcatgtccct gagtttttca
      901 ccctgccgcc cgaagtcctc cctcccccgt ccggccccga gtccaattca cgacgcattt
      961 tcgccacccg caaggagcag ggggtcgcca ccacgtcggt cgcgcgtttc caacgctgtt
     1021 ttctcgcggt gccccatggt tattccagcg ccaagacggc acgagcgcta gctctcgaag
     1081 acctgacccc cattctcgat ctggcccaga agcatgggct gaagcgagtc atcgtgccgg
     1141 tatccgaccc cgggttgttt ctcgacgcgc agtctcaacg cgtgttccag cagaagttcg
     1201 tccagttcca tctcgaagcg gagaagcgcg ggatcaagct gctggtaaaa aatggcggcc
     1261 tttcgaaaga tctgtttctc aaactgcgca aggagaccgg gtgcaagctc gcgttcaatc
     1321 tgggcacggc ctttttcgag cgcctcgacc tgctcgaatc gctgcgcgag cagcaggaat
     1381 atatcggcgt gctgtttctg catcaggctc tctcgggaat cgacgtgctc aacgagtgga
     1441 ccgatcgcgt gcgcaagacg ctgaaccaga cgatcgacgc ggtcgccgcc tatcagaacc
     1501 cggaaaacca gaaagatccc gctgataccg aatattatct cagtcagttg cgcaaggcgt
     1561 tcttcagctt tcgcgacgcg cggcagaaca gcaccacgca tctgggcatc ttccagcacg
     1621 gggccatcaa ctttgtccca gtcctcaaat atctgaaaca gctgatagag cagggatcag
     1681 acctcaccgt tttcttcgaa gcggtaccca atctcaagaa cctcgagttt tttgaacgct
     1741 atgtggtctc agatattctg ccggctccga tctgaagcga tgctgcgccg gctttctgtg
     1801 accgaaaagc gcagaacggg gtttgcccgc ctgctgccgc tgctgctgtt caccatctgc
     1861 tggtttcctg cggttcccgc gggcgctgcc tcgcccctgc ggaacgcagt cgcaaccgac
     1921 gaaccggcga tcagccgccc ctacacgctg caggaaaccc tgcgacgcgc cgaggagctg
     1981 tttctgcaga acaactaccg tgaagcgctg gtgttttact acgaagggct cgaccaggtc
     2041 gaagccaacg agattcgcgg caagctgcat ttccgtatcg gcgaatgtct cgagggcata
     2101 cgccgtttcg atttcgcggc ctaccactac aaactggcgg tgaaagcgaa cctgggtcgc
     2161 gggcttaccg agcgggccgt caacaagctg aaactgctgc ccaagctggc gcagaaggaa
     2221 gaggccatgc gtttgtacca gcgggctatg gccgcctacc gcaagcgcaa cgtgcgcgac
     2281 gccattgatg attatctcaa gagtctgcga ctcgaaccct cgctgatggc acaggacaat
     2341 tccggcctgc tcgacgacgc gattcagtat ctgatgctgc tgtctgagtc gaaagaccag
     2401 gaaccgtcac gcctgctgaa acttgcctca cttctcgaac tgcgg
//
LOCUS       DAORHD010000764         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52218_length_2445_cov_3.296234, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000764 DAORHD010000000
VERSION     DAORHD010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52218_length_2445_cov_3.296234"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PKO06_15110"
     CDS             complement(<1..287)
                     /locus_tag="PKO06_15110"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71030.1"
                     /translation="MAAHVYSESLLDEVIDACINHLRDHPKDHETLFHLGNAFFLRGL
                     FEKASYCFVKAINVNPKNPAYYSHLGHTYRQLGDRDQAVSSYEAVLSLNPK"
     gene            complement(291..1010)
                     /locus_tag="PKO06_15115"
     CDS             complement(291..1010)
                     /locus_tag="PKO06_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71031.1"
                     /translation="MNEMENIQETFAFFSVRKEQETLQTVFTAILALPVDQVSVEFPN
                     RPDEQFKHINDRSRKILMSLPSQQKISRISGDFSDGGSFSLHGSTLLITLSSDQYNFE
                     VSDTMRKVLSPVFPLCIFRNPYIWGIDLYLPYERQTLFDTRNYIVRSQLLEDPQIDIF
                     RRDDGIIFKLRFHLAGDHEFEEGMRLLHALFEEIHETLAKGSYEPLELLHLYCADRRQ
                     YRSFSPRTKLGNRFKAIIADT"
     gene            complement(1007..>2445)
                     /locus_tag="PKO06_15120"
     CDS             complement(1007..>2445)
                     /locus_tag="PKO06_15120"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71032.1"
                     /translation="LFAALLVGILCYWWYCWRPIVEEIEQMQREKISSTESEDFFFQP
                     IKLVGWLEENISRLKNYYSDLERVSQLISFINYSYELPRQLKVALNLAHEVFPRVTIL
                     VFLPQKNQVRFEVGTRINSSGILEPLAREDGIVARALDEVNHSIDLSKLHENNWRSFT
                     LPVKLQAAPDYQIMPLTLWNRVLGLVVYDTSERHRPFDNDERVMAVLINRQMALFIEN
                     HLLYAEKVQQERLHREVEIARQVQTESLPSTVSPIPGYDIFGLCNPCHEISGDYYDLI
                     PLGDGRALIALADVSGKGLPAALFLSKIQTLVRALAHQYKSPAQLLSFLSKHLSAEKM
                     GALFATMVIVIIESGSTQAICSSAGHCKPLIVRPGIGLVEELNFEVGIPLGLFDMGEE
                     GYSDILIDLIPGDGIFLYSDGVSDLTNAARERYGTERVRRDLDDAPRNRAAAAVYHLL
                     ERMNRFKGQSPLEDDVTMVFIKSEPRRP"
BASE COUNT          547 a          629 c          724 g          545 t
ORIGIN      
        1 tacttggggt tcagcgacaa taccgcttca tacgaagaga cggcctgatc acgatcgccc
       61 agctggcggt aggtgtgccc gagatgcgaa tagtaggcgg gatttttcgg attgacgttg
      121 atggctttga cgaaacagta cgaggccttt tcaaacaatc ctctgaggaa gaaagcgttt
      181 ccgaggtgga acaacgtctc gtgatccttg gggtgatcgc gcaggtggtt gatacaggcg
      241 tcgatgacct cgtcgaggag tgattcacta tagacgtgtg ccgccatggt tcaggtatcc
      301 gcgatgatgg ctttgaaacg atttcccagc ttggttctgg gtgaaaacga ccggtactgt
      361 cgtcgatctg cgcagtacag gtgcaggagt tcgaggggct cgtagctccc cttcgccagc
      421 gtttcgtgga tctcctcgaa gagcgcatgc aacaagcgca tgccctcttc gaactcgtga
      481 tcaccggcca gatgaaagcg cagcttgaaa atgatgccgt cgtctcgccg aaagatgtcg
      541 atctgcgggt cttccagcag ctgacttctg acgatgtaat tgcgggtatc aaaaagcgtt
      601 tgtcgttcat acggcaggta caaatcgata ccccagatgt aggggttgcg gaagatgcag
      661 aggggaaaga ctggtgacag aaccttgcgc atcgtatccg acacttcgaa attgtattgg
      721 tccgatgaga gcgtgatcag gagcgtcgaa ccgtgcagcg agaagctgcc gccgtcactg
      781 aagtcgcccg aaatgcggct gatcttctgc tgtgagggca ggctcatcag aatcttgcga
      841 ctgcgatcgt taatatgctt gaactgttcg tcgggccggt tgggaaactc gaccgagact
      901 tgatcgacag gcagagccag aatggcggtg aatacggtct gcaaggtttc ttgttccttg
      961 cgaacactga aaaaggcgaa agtttcctga atattttcca tttcgttcat gggcggcggg
     1021 gctccgattt gatgaagacc attgtgacat cgtcttcgag gggtgattga cccttgaacc
     1081 ggttcatcct ttccaagagg tgatacacgg cggcggcggc ccggttgcgc ggtgcatcgt
     1141 cgaggtcccg tcgcactctc tctgttccat accgctcccg ggcggcgttt gtcaaatccg
     1201 acactccatc agagtacaaa aagatgccgt ccccgggaat gaggtcgatc aggatgtccg
     1261 agtacccttc ctcgcccata tcgaacaaac ccaggggaat tccgacctcg aaattgagtt
     1321 cttcgaccag gccgattccc gggcgtacga tcaggggctt gcagtgaccg gcacttgagc
     1381 agatcgcctg ggtcgagccc gattcaatga tgacgatcac catggtcgca aagagcgcgc
     1441 ccattttttc cgcgctgagg tgtttcgaca gaaacgagag cagttgagcg gggctcttgt
     1501 actgatgcgc cagcgctcga accagggtct gaatctttga cagaaagagg gcggccggca
     1561 ggcctttgcc cgagacgtcc gccagtgcga tcagcgcgcg accgtcaccg agcgggatca
     1621 gatcgtagta gtctcccgaa atctcgtgac aggggttgca gagaccaaag atgtcgtatc
     1681 cgggaatggg cgagacggtc gacggaagtg actcggtctg cacctgccgg gcaatttcaa
     1741 cttcacggtg aagtcgttcc tgttgcacct tttcggcata gagcagatgg ttttcaatga
     1801 acaaggccat ctggcgattg atcagcaccg ccatcactcg ttcgtcgttg tcgaacggcc
     1861 gatgacgttc gctggtgtca tagacgacga gccccagaac tctgttccag agcgtgagcg
     1921 gcatgatctg gtagtccgga gccgcctgga gcttaacggg gagggtgaag gaacgccagt
     1981 tgttttcgtg aagcttggaa agatcgatgc tgtgattgac ttcatcgagg gcgcgggcca
     2041 cgatcccatc ctcgcgtgcc agaggctcca agatccccga agaattgatg cgggtgccga
     2101 cctcgaatcg cacctggttt ttctgcggca gaaacacgag aatcgtcacg cgaggaaaca
     2161 cttcgtgggc gaggttgaga gccactttca gctgacgcgg cagttcatat gagtaattga
     2221 tgaagctgat gagctgcgag actcgttcca gatcagagta atagtttttc agacgcgaga
     2281 tgttctcttc cagccagccc accagcttga tgggttgaaa gaagaaatcc tcactctcgg
     2341 tgctcgagat tttctcgcgc tgcatctgct cgatctcctc gacgatgggc cgccagcaat
     2401 accaccagta acagagaatg ccgaccagca acgccgcgaa caggg
//
LOCUS       DAORHD010000765         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52225_length_2445_cov_2.792050, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000765 DAORHD010000000
VERSION     DAORHD010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52225_length_2445_cov_2.792050"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PKO06_15125"
     CDS             <1..511
                     /locus_tag="PKO06_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016526303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV71033.1"
                     /translation="IVSTARPRVRCPLFDPPQSTFQPTRKEANVPNINTIPGEDVFYM
                     DSRVMPGIPLDDVVAWCRSRADEVAARHGVSIVIEPVQFEQAAPPTPADSPLVRSLQH
                     AIREVHGREAVPMGIGGGTVAAYFRRHGLHAAVWSTVDESAHQANEYCRLPNLLADAR
                     VFARVLATL"
     gene            527..>2445
                     /locus_tag="PKO06_15130"
     CDS             527..>2445
                     /locus_tag="PKO06_15130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV71034.1"
                     /translation="MSSAAPTSSSYRVFLASIILCVTLTVGILFQGFSWSHSVLSPEE
                     STELKDAVRTAPGQFWSAIRASRSVRDQSGRLRGALAWLADAERDGNGPERNNALIAR
                     ADLLFRHGYPEQALVELNRAMEPVSEEWGRTSYWWLKAELEHHLGLNDALASSTLMLL
                     ETGYDADEVDSTLAQRSPTPRLSLPQPLILPFWIFLLLFPSFHLWLTPAPKERSASSH
                     LGQQRFGALRLGLAMIVVSTILIWVLRFPNAVFPEYPLTALLIHLASAYTLILYPAFR
                     HDRGERQTTWSFPAFARFVSGWLIILLAMPLVFLLSWLLIREMALKLPFWAVLHPAGP
                     ALGLPALSGFLLLLLPWGIPLLLGWRSLRANDPQAALLADLPCPVYLWDLPGSRFANA
                     LTIGYLSPSTAIGFTQPLLDGLTGEQIRSIARHEVGHVHHRHLLLYFLVTMPAYLIIG
                     LFSSLNPLGLHRHLVFGPSLPFALALILGFLLWLVLFASISRRCEQEADRFAATDGKR
                     EIFLRALTRLYELNLMTGDALAPAQTKLSSHPGLQERKRMLRETEGQPVFPTAVSTDL
                     LLIACWRARLALEWKGSLTEATNLVAFEFDSSSNLLQERIAHCADRHAQLGSECLILP
                     DAHGLDVLFCAQKNAV"
BASE COUNT          428 a          802 c          657 g          558 t
ORIGIN      
        1 catcgttagc acggcgcgcc cgcgtgtccg atgcccgctg ttcgacccgc cgcaatccac
       61 ctttcagcca acgcgcaagg aagcgaacgt tcccaacatc aacacgattc ccggcgaaga
      121 tgtcttctac atggacagtc gggtcatgcc cggcattcca ctcgacgacg tcgtagcctg
      181 gtgtcggtct cgcgcagacg aagtcgctgc ccggcatggc gtttcgattg tcattgaacc
      241 ggtacaattc gaacaggcgg caccaccgac cccggcagac tctccgttgg tacgatcgct
      301 gcagcatgcc attcgagagg tgcatggccg cgaggccgtg ccgatgggca tcggtggcgg
      361 caccgttgcc gcgtattttc gtcgtcacgg actgcatgcc gcggtctggt caaccgtcga
      421 tgagtcggcg caccaggcaa acgaatactg ccgcctgccg aatctgctcg ccgacgcccg
      481 ggtgttcgcc cgggtgctgg ccaccctctg accgatgcca catcgcatgt cgagcgccgc
      541 acccacttct tcaagctatc gagtgtttct ggcttcgatc atcctgtgtg tcacgctgac
      601 ggtcggcatt ctgttccagg gcttctcatg gtcacattca gtcctgtcgc ctgaggaaag
      661 caccgaactg aaagacgcgg tgcgaactgc gccggggcag ttctggtctg ccattcgcgc
      721 ttcccgttcc gttcgcgatc aatcagggcg cctgcgtgga gcactcgcgt ggctcgccga
      781 cgcggaaaga gacggcaacg gaccagaacg gaacaacgcg ctcatcgcac gggccgatct
      841 gctctttcgc cacgggtatc ccgaacaggc cttggtcgaa ctcaaccgcg cgatggaacc
      901 cgtaagcgaa gagtggggac gcacctccta ctggtggctg aaagccgaac tcgagcatca
      961 tctcggcttg aacgacgcgc tggcatcttc gactctcatg ctgctcgaaa ccggatacga
     1021 cgcagatgaa gtcgattcga ccctggcaca acgatcgcca accccaagac tgtctctgcc
     1081 gcagcccctg atactcccct tctggatctt cctgttactg tttccgagct ttcatctctg
     1141 gcttactccc gctcccaaag agcgttccgc atcatcgcac ctgggacaac aacgcttcgg
     1201 tgccttgcga cttggtctgg cgatgattgt cgtcagtacg atcttgatct gggtgcttcg
     1261 ctttcccaat gccgtttttc cagagtatcc tctgacggcc ctcctgattc atctggcgag
     1321 cgcgtatacc ctgatcctgt atcccgcctt ccggcacgat cgtggggaga gacagaccac
     1381 ctggagcttt cccgccttcg cacgctttgt ctctggctgg ctgatcattc ttctggccat
     1441 gcctctcgtg tttctgcttt cgtggctgct gatcagagag atggcattga aactgccatt
     1501 ctgggcagtc ctgcatccag ccgggcccgc tctcggcctg cccgccctct ccgggttcct
     1561 gctgctcctt ttgccctggg ggattccgct tctcctcggc tggcgcagtc tgagggcaaa
     1621 cgatccccag gcggcgcttc tggcagacct cccttgtccg gtttacctgt gggacctgcc
     1681 aggcagtcgt tttgccaatg cactcaccat cggctatctc agcccatcga cagccatcgg
     1741 cttcacacaa ccgttactcg acgggctcac cggcgaacaa atcaggtcca ttgcccgtca
     1801 tgaagtcggg cacgtacatc atcggcatct gctgctgtat tttctggtca cgatgcccgc
     1861 ctatctcatc atcggtcttt tctcctctct caatccgctt ggtttgcatc gtcatctggt
     1921 ttttggcccg tcactcccgt tcgccctggc tttgatcctc ggttttctcc tgtggctggt
     1981 gctgttcgct tccatcagtc gtcgctgtga acaggaggct gatcgctttg ctgcgaccga
     2041 tgggaagcgc gaaatcttcc tgcgcgcact gaccagactc tacgaactca acctgatgac
     2101 cggcgacgca ttggccccgg cccagacaaa gctttcctcc cacccgggtt tacaggaacg
     2161 aaagcgtatg ttgcgagaaa ccgagggaca accggtgttt ccgactgccg tcagcacgga
     2221 cttgctgctg atcgcctgct ggcgcgcacg gttggctctt gaatggaaag gcagcctcac
     2281 cgaagcgaca aacctggtcg ctttcgagtt cgattcaagc tcaaacctgc ttcaggagcg
     2341 aatagcacac tgcgccgacc ggcacgctca actcgggtcc gagtgcctca ttctgccaga
     2401 tgcccacggt ctcgacgtgc tcttctgcgc gcagaagaat gcggt
//
LOCUS       DAORHD010000766         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52240_length_2444_cov_3.454165, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000766 DAORHD010000000
VERSION     DAORHD010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52240_length_2444_cov_3.454165"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKO06_15135"
     CDS             <1..283
                     /locus_tag="PKO06_15135"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNV71035.1"
                     /translation="LCQGLHFTTSVPLLGNAVYLGLVLIQTLLQTHADVIQFPKRPDR
                     VQPFFDLLAGNAWIREGLLQGIPAVDLAARAQTEVADFEAQARQFQLYQ"
     gene            complement(394..1230)
                     /locus_tag="PKO06_15140"
     CDS             complement(394..1230)
                     /locus_tag="PKO06_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71036.1"
                     /translation="MTRLVLALVLVLVCCSFVSAAPNAAVNIKRDTVALVAECPATEV
                     LTLRVQGRKAWLVCRNGDFWNVDLETGSVSERRSLAKPVVDAALLGEELIGLEGSGAL
                     LGKVPTHWTTAPVPAVRLESAGGDLLIVSDTESRYLAAPATQSIRLGPLPLAVPFRDG
                     FLWSMSRQTGGRAWQIELLDMFGNRMKRLYRFSAEFDPTGTVMGPVGPEGEILISFYT
                     GQTREFAAIGPHGRMLWRVPGSAPVCARDFAWSPTGGLLVLEAQGTGVAVTRWQFVTP
                     EG"
     gene            complement(1227..2201)
                     /locus_tag="PKO06_15145"
     CDS             complement(1227..2201)
                     /locus_tag="PKO06_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71037.1"
                     /translation="MIRVLTLWCAFMLVLQTSVVNAGSALRLAYGKGAGQVSFFNPSV
                     TQNYEEATPLGPMTFRRHGETFWVADSIAGRVLCVSEIGKVLHSLTIPGVPDNTLLED
                     LALVPGPDGRIRSVYVADGADLIVRQIEVASGKELGRFGGRGERPGQFLQIHQLEVGP
                     SGRIYVGDYGRRVVAVFEPDGKLVRELPWQLTGFLVTEQDGLIMITHTDNAGFFLKEY
                     DTAGQPVRSVHLGQSGSMNARIWGQAAEGGYWVTLTPASGFKGYLQLVRFSHFGAVAE
                     KRLIRPVLGMNRFLDLTSGRDLWLARADYGAAPRGAFIIEALTREEKK"
     gene            complement(2207..>2444)
                     /locus_tag="PKO06_15150"
     CDS             complement(2207..>2444)
                     /locus_tag="PKO06_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71038.1"
                     /translation="LKAHGWKEVSAPPYREGDVITWKTYDYTGDGVKDPDTHIGIILK
                     EGGSFKAMNNSSRLRTPRITDIGIAPVTRVLRKE"
BASE COUNT          526 a          760 c          691 g          467 t
ORIGIN      
        1 cctttgtcag gggctgcatt tcacgacctc ggtccccctt ctcggcaacg ccgtctatct
       61 cggcctggtg ctgattcaaa cgctgttgca aacccacgcc gacgtgattc aatttcccaa
      121 gcgccccgat cgtgtccagc ccttctttga tcttctggcc ggaaacgcgt ggattcgtga
      181 aggtctgttg cagggaatcc cggccgttga tctcgcggca cgtgcccaga cagaggtcgc
      241 cgatttcgag gctcaggcca ggcagtttca attgtaccag tgagtgtttc gtgttctcgg
      301 ctttgaaaga gcttcacgcc tgatagaaaa acaaccccgg agagtcgggg ttgttgcagt
      361 cggacggttg tggacggaga actcgagatc ttctcagcct tccggggtga cgaactgcca
      421 acgggtcacg gccactccgg ttccctgggc ttcgagcacc agaagaccac cggtcgggct
      481 ccaggcaaaa tcacgcgcac agacaggcgc tgagccagga actcgccaga gcatgcgccc
      541 atgcggcccg atcgccgcaa attcgcgggt ctgaccggta tagaacgaaa tcaaaatctc
      601 gccttccgga ccaactggcc ccatgacggt tccggtgggg tcgaactcgg cactgaaccg
      661 gtacaatcgt ttcatgcgat tgccgaacat atcgagcagt tcgatctgcc aggcacgccc
      721 ccccgtctgc ctgctcatcg accagagaaa accatcgcga aacggtacgg ccagtggaag
      781 cgggcccaga cgaatcgatt gggtcgcggg cgccgcgagg tatctcgact ccgtgtccga
      841 gacgatcaga aggtcaccac cagcgctttc caggcggact gcgggaaccg gagccgtggt
      901 ccaatgggtc ggcactttgc cgagaagtgc gccactgcct tcaagaccga tgagttcttc
      961 ccccagcagg gcggcatcaa ccaccggttt cgcgagactg cggcgttcgc tgacactgcc
     1021 ggtctcgaga tcgacgttcc agaagtcgcc attgcggcac acaagccagg cctttcgccc
     1081 ctggacacgc agcgtcagca cctcggtggc cggacactcg gcaaccagag ccaccgtatc
     1141 gcgtttgatg ttcacagcgg cattcggagc ggctgagacg aaactacaac agaccagaac
     1201 gagaacaagg gcaagaacca agcgggtcat ttcttttcct cccgggtgag ggcttcgatg
     1261 ataaacgctc cgcggggggc ggcaccgtag tcggctcggg ccagccagag atcacggccc
     1321 gatgtcaaat caagaaagcg gttcataccc agcaccggtc ggatcagtcg cttttccgcc
     1381 acggccccga aatgtgaaaa ccgcaccagc tgcagatacc ctttgaaacc actcgccggg
     1441 gtcagcgtca cccagtagcc accttcagcg gcctggcccc agatacgagc attcatggac
     1501 cccgactgtc cgaggtgaac cgagcgtacc ggttggccgg ctgtgtcata ctctttcaga
     1561 aagaagcccg cgttgtcggt atgggtgatc atgatcaggc catcctgttc ggtcacgaga
     1621 aacccggtca gctgccaggg aagctcacga accagcttgc catcgggctc aaaaacagcc
     1681 acgacccgtc gaccgtagtc accgacgtag atgcggcccg acggtccgac ttccagctgg
     1741 tgaatctgca gaaactgacc gggtcgctca ccgcgcccgc cgaagcgtcc cagttccttt
     1801 ccgctggcaa cctcaatctg tcgaacaatc aggtcagcac catcagcgac gtagaccgag
     1861 cgaattctgc catccgggcc aggaacgaga gccagatctt cgagcagtgt gttgtcggga
     1921 acccccggaa tcgtcagtga atgcagcact tttccgattt cggacacgca cagcacgcgc
     1981 ccggcgatgg aatcagcgac ccaaaaggtt tctccatgac gtcgaaacgt catcggcccc
     2041 agcggtgtcg cttcctcgta gttctgggtg acggacggat tgaagaacga gacctgacca
     2101 gcacctttcc cataggcaag tcgcagagcc gaaccggcgt tcacgaccga agtctgcaga
     2161 accagcatga atgcacacca gagagtcaga acgcgaatca tgatcctcat tcctttcgca
     2221 gtacgcgggt gacgggcgcg atgccgatat cggtaatacg gggagtgcgc agacgcgagc
     2281 tattgttcat cgccttgaag cttcccccct ctttcagaat gatgccgata tgggtatcgg
     2341 gatctttcac gccatcgccc gtgtaatcgt aggttttcca ggtgatgacg tccccttcac
     2401 ggtacggagg cgctgagact tctttccatc cgtgcgcttt cagg
//
LOCUS       DAORHD010000767         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52285_length_2443_cov_2.816583, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000767 DAORHD010000000
VERSION     DAORHD010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52285_length_2443_cov_2.816583"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            492..773
                     /locus_tag="PKO06_15155"
     CDS             492..773
                     /locus_tag="PKO06_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006257046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV71039.1"
                     /translation="MNKADLVKAISNKAKLNQAAAQKALEAMLDTFKDTLKKGNKIQL
                     IGFGSFEVAKRAARKGVNPQTKKPISIAAKKVVKFKPGKELKEIVSKSK"
     gene            990..>2443
                     /locus_tag="PKO06_15160"
     CDS             990..>2443
                     /locus_tag="PKO06_15160"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71040.1"
                     /translation="MSQTQNDQPNTEGKREHWGSRIGLILAMAGNAVGLGNFLRFPTQ
                     AASNGGGAFMIPYFIAFLLLGIPLMWIEWGIGRYGGSIGHGTVPGIFHKMAPRHRWVK
                     YIGVLGVILPLFITVYYNYIESWTLAYGYFSISGKYDVQGADLATRRAEMKRFMNEYR
                     GIPSTVQLHREVTVNEIEQVRAFVEPASVPTQLLYNPDKHGTWDAMNPKADQAKKPFR
                     SIALQPKYFDGLSTAYVFFLIAFACNFYFVYRGLSSGIEQLGKIGMPILFAFAVILGL
                     RVLTMDTPVGSSYSVADGFSFLWTPRFSDLASASVWLAAAGQIFFTLSLGTGAIHAYA
                     SYLSEKDDIVLSGLATASTNEFAEVVLGGTIAIPAAVALFGPQMTQEIARGGSFDLGF
                     QSLPMVFTQLSGGMLMGAMWFILLFIAGITSSVALLMPAVTFLKDELKWAHGKAVTAV
                     LLLSGVCSHWCIFYISRGVLDEIDYWAGTFLLVV"
BASE COUNT          529 a          611 c          706 g          597 t
ORIGIN      
        1 gactcatgac accaggctgg tgtcagaatc ggtgcgtcgg cttactcaat aggtaagcat
       61 gaaagccagg tgcctgtctg ggagattgag agggagtcgt gacaaggcgg aatgaatgcc
      121 gggagataac agtggcgtgc gggggtatgc tgcgctcagt gtgttggaag agctggcgaa
      181 tgatgtttca aggccagtcc aatggctgaa aacgatgttt ggcgggggtg cgctgacctt
      241 gaatcgggag acaggaatgc cgagcacatc tgccgacttc aaacagggga tttttatgcg
      301 gcatgctgtt ctcccgggtc gttcatcggg aaaaaagcgc gcggaaacgc tctcctgaag
      361 cgtgcatcgg gagattgcga taaataatga aaatattttt gtcaggggta ttgacacaat
      421 gaatcgatgc tgcatataat caacccatag tcctacaaac caaatcaaat ctctaaagga
      481 gaacgggtag aatgaataaa gcggatctcg tgaaggccat ttccaacaag gcgaagttga
      541 atcaggctgc tgctcagaag gctctcgagg ctatgctcga cacgtttaag gataccctga
      601 agaagggcaa caagattcag ctcatcggct ttggctcatt cgaagtggcc aagcgcgcgg
      661 ctcgcaaggg cgtcaatcct cagaccaaga agcccatcag catcgccgcg aagaaggttg
      721 tcaagttcaa gcccggcaaa gaactgaagg aaatcgtctc caagagcaag taagtctttt
      781 ctaaaagaca caaaaatggc cccggtgtac accggggctc tttttttgtg tctttttgat
      841 tcgttaagaa acatttcatt gcgaatcgtt cggtttccgt tgcctgctgt acatggttgt
      901 gttactgtac cccttttctc gggcaatcac aggttatgta cactgcggcc cgagccgttg
      961 atgaagtcga acgcgagtgg gaggaaagca tgagtcagac gcaaaatgat cagccaaaca
     1021 ccgaaggcaa acgcgaacac tggggaagcc gcatcggcct gattctggct atggccggaa
     1081 atgccgtcgg tctcgggaat tttctccggt ttccgacgca ggccgcctcg aatggcggtg
     1141 gcgcgttcat gattccgtat tttattgcct ttttgctgct cggtattcca ttaatgtgga
     1201 tcgaatgggg tattggcagg tatggtggga gcattggtca tggtaccgtg cccggcatct
     1261 tccacaaaat ggctccgcgc caccggtggg tgaagtacat tggcgtattg ggcgtcattc
     1321 tgccgctgtt catcacggtg tattacaact acatcgaatc ctggaccctc gcgtacggct
     1381 atttttccat ctctggcaag tatgatgttc agggagccga tcttgcgacc cgtcgcgctg
     1441 agatgaagcg cttcatgaac gagtatcgcg gcattccatc tacggtgcaa ctgcatcgcg
     1501 aagtcaccgt caatgagatc gagcaggtgc gcgcgtttgt cgaaccggct tctgtaccga
     1561 ctcagttgct ctataaccct gacaagcacg gaacctggga tgccatgaat ccgaaggcag
     1621 accaggcgaa gaaaccgttc cggagtatcg cgctgcagcc gaagtatttt gatggcttgt
     1681 ccacggccta cgtcttcttc ctcatcgctt ttgcgtgcaa cttttacttt gtgtaccgcg
     1741 gcttgtcttc agggatcgag caactcggaa aaatcgggat gccgattctt ttcgcgttcg
     1801 ccgtgattct gggcttgcga gtgttgacca tggatacacc ggtcggatcg tcctactcgg
     1861 ttgccgatgg attcagcttt ctgtggactc cgcggttctc ggatctggcg tcagcctcgg
     1921 tctggctggc ggcggcagga cagatctttt tcaccctgtc gctcggcact ggggcgattc
     1981 acgcctacgc cagctatctg tcagagaagg acgacatcgt tctctcgggt ttggcgactg
     2041 cttcgaccaa cgaattcgcc gaggtcgtcc tcgggggtac gatcgcgatt ccggcggcgg
     2101 tcgcgctttt cgggccacag atgacccagg aaatcgcgcg cggtggatcg ttcgatctgg
     2161 gatttcaatc gctgcccatg gtgtttacgc agctcagcgg gggtatgctg atgggagcga
     2221 tgtggttcat tctcttgttc atcgccggta tcaccagctc cgtcgcgttg ctcatgcccg
     2281 cagtcacctt cctgaaggat gagttgaaat gggcgcacgg aaaagccgtc accgccgtgt
     2341 tgctgctttc gggagtatgc tcgcactggt gcatcttcta catcagtcgc ggcgtgctcg
     2401 acgagatcga ctactgggcc ggaacctttc tgctcgtggt gtt
//
LOCUS       DAORHD010000768         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52343_length_2441_cov_3.421207, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000768 DAORHD010000000
VERSION     DAORHD010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52343_length_2441_cov_3.421207"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..298
                     /locus_tag="PKO06_15165"
     CDS             128..298
                     /locus_tag="PKO06_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71041.1"
                     /translation="MKTLAERPRIEITLDEDGESIRIEQENESDEVDCIIIPICDIPL
                     FIEKLKAEAGLK"
     gene            complement(516..800)
                     /locus_tag="PKO06_15170"
     CDS             complement(516..800)
                     /locus_tag="PKO06_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71042.1"
                     /translation="MRAQVRAAISLLLTLLSLSSIVLVNAGQQADQMPDDEPLKQFLL
                     QLEAKCAAIADLECELRETFLSDGKPERTSRFRRFATCRVPAVLSWATNS"
     gene            complement(785..1024)
                     /locus_tag="PKO06_15175"
     CDS             complement(785..1024)
                     /locus_tag="PKO06_15175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV71043.1"
                     /translation="MPIYEWICQSCSQTFETNSSMRELTRPDCPTCHGSDVRRRFSAT
                     VGVIIRPSASTKVCRNQQTCQGPTPRCDGHTCERK"
     gene            complement(1134..1475)
                     /locus_tag="PKO06_15180"
     CDS             complement(1134..1475)
                     /locus_tag="PKO06_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71044.1"
                     /translation="MKIKCECGMLIHDTGCESPHVGAVISSADVAFLLDRLDECVENA
                     EPTPAARERACMNLRALWSRVSRKVYQCPRCSRLFIDDEQRELRTFIPERFGTPCDLL
                     RPKNSEPWQAY"
     gene            1688..>2441
                     /locus_tag="PKO06_15185"
     CDS             1688..>2441
                     /locus_tag="PKO06_15185"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV71045.1"
                     /translation="MTPVPPSSNRLRFFSNWRWMAVFLLVLFAVSYLLFQTLLSIFVE
                     YADQQRHASLREKVRMARSAIEPTLRDLRTGTITKTQAIEKVQALLNTLTFEDEFGLN
                     YIFLNTTDGTVLVRPYRPQDVGKNMIDIQDSTGKYYMRELPTVARANSDGGFCRYQFP
                     HPGTQQDEEKLSFALLVPELDAVLGTGSYMSKATQAKVALLHRAFGIGLCLYLLFGLP
                     LLVSMYQLQRQNVLMQQEIEQRHRTEQELVRNE"
BASE COUNT          545 a          700 c          681 g          515 t
ORIGIN      
        1 ggcgttgtga tttggggcca cacaggatat gctggccgag atgatcgcag gctccagggt
       61 cctgattcca tgatgtttcc ggaagcctgc agggtgcgat cacgacattt ccgccgcgga
      121 gggactcatg aaaacgctgg ccgaacgacc gagaatcgaa attacgctcg acgaagacgg
      181 cgaatcgatt cggatcgaac aggaaaacga aagcgacgag gtcgactgca tcatcattcc
      241 catctgcgac attccgttgt tcatcgaaaa actgaaagcg gaagcgggct tgaagtagca
      301 ggtttccgca cacttccacg cctggcctgc cggcagacgg ctgaaagacg aatgggaatc
      361 cccctgaaac ctcgcgtcat cgaagcgaga gtcggcaaga gcctcgcggt cggcggggaa
      421 acacttgcgg aaccactcga cggcgggctg ggtgtcacct gatttgtccg attcaggatt
      481 tttcagcaga ggctttttat ctgcttagaa gcggatcaag agttcgtagc ccatgaaagc
      541 accgccggaa ccctgcaggt cgcgaaccgc cgaaaccggg atgttcgttc gggcttgcca
      601 tcactgagaa aggtctcgcg cagttcgcac tcgagatcag cgatcgcagc acacttcgcc
      661 tcgagttgaa gcagaaattg tttcagaggc tcgtcatcag gcatctgatc agcctgttgt
      721 cctgcgttga ccaggacaat agaagaaagg gaaagcagcg tcagcagaag gctgatcgcg
      781 gcccttactt gcgctcgcac gtgtgtccgt cgcagcgtgg ggtcggcccc tgacaggtct
      841 gctggttacg gcaaaccttg gtcgaggccg agggtcgaat aatgaccccg accgtcgccg
      901 agaatcggcg gcgaacgtca cttccgtgac aggtcggaca gtccgggcgg gtcagttccc
      961 gcatcgagga attcgtctca aatgtctgtg aacatgattg acagatccat tcatagatcg
     1021 gcatcagagg cctcctggat acagatgtcg ttatggaatc acctatcgat agccgtggca
     1081 tctcctctcg tcaggcacga actcggagag tggccatcag agggaaaggg ggttcaatag
     1141 gcttgccagg gctcagagtt cttcggtcgc agcagatcgc aaggtgtacc gaagcgttca
     1201 ggaatgaacg tgcgcaactc acgctgttcg tcgtcgatga agaggcgtga gcagcgagga
     1261 cactgataaa ctttgcgact caccctggac cacaacgccc ggagattcat gcaggcacgt
     1321 tcacgcgccg caggggtcgg ttcggcgttt tccacgcatt catccaggcg atcaagcaga
     1381 aaggccacat cagcgctgga gataaccgca ccgacgtgcg ggctttcaca cccggtgtcg
     1441 tggatcaaca tgccgcactc acacttgatt ttcatcgcca ctccccttct ccgctcaggt
     1501 tccgactcga tgccggcgta cggtcatcac cccagggcca tcggaaaccc catcgaacgt
     1561 gacttccgtt ggcatcgcat cgcttcacca tccacgaaaa gtgtatcatc attccggcac
     1621 cctgtgctag gatacggacg gcctttcata caggactacc cctgatcgtt gagagtcatt
     1681 cgtacccatg acacccgtgc ctccgtcttc gaaccggttg cgcttttttt ccaattggcg
     1741 ctggatggcc gtttttctgc tcgtcctgtt tgccgtttcg tatctgctct ttcagaccct
     1801 cctttccatt tttgtcgagt atgccgatca gcagcggcac gcaagcctgc gcgagaaggt
     1861 tcgtatggcg cgcagcgcca ttgagccaac ccttcgcgac ttgcgcactg gtactatcac
     1921 caagacgcag gccatcgaaa aagtccaggc actgctcaac accctgacct tcgaagacga
     1981 gttcggtctg aactacatct tcctcaacac aaccgatggc accgtgctgg tgcgtcccta
     2041 ccgtccacag gatgtcggaa agaacatgat cgacattcag gattcgacgg gaaaatacta
     2101 catgcgcgag ttgccgaccg tggcccgcgc caactccgac ggtggcttct gccgatatca
     2161 gtttccgcac cccggaaccc agcaggacga agagaagctc tccttcgcac tgctggtacc
     2221 ggaactcgat gccgtgctgg gaacgggcag ttacatgagc aaggccacgc aggccaaagt
     2281 ggctctgttg catcgagcat tcggaatcgg tctctgtctg tatctgctgt tcgggttgcc
     2341 acttctggtt tcaatgtacc agttgcagcg acagaatgtt ctgatgcagc aagagattga
     2401 gcaacgtcat cgcaccgagc aggagttggt tcgcaacgag g
//
LOCUS       DAORHD010000769         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52357_length_2441_cov_2.403604, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000769 DAORHD010000000
VERSION     DAORHD010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52357_length_2441_cov_2.403604"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PKO06_15190"
     CDS             <1..1233
                     /locus_tag="PKO06_15190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71046.1"
                     /translation="HHLHSWFGNRMPETDQEGLIVSELGNLQRQYHPPFVLFAFDPMD
                     YGYGFTPVFTPTDPALADTQYFSRTLDAPFQSTIGSGTTEALHLVHPVQDHTKAVVSC
                     VLPLGRERLLQEWEHQLNLWLAGAAFLLTIGMAMLAARRYTAPLLVLSEGARLIESGD
                     YRVRLAVGQDDEFGLLARHFNAMAHGVEEGRLLSRFVSESVREAAQSRDLEQQAQRGT
                     SEEVVVVFVGLSTFSQLLNSLPPERLIDHLNRYLGVMARQIREQGGRIDKFIGDKILA
                     VFRPRDFSGPAAAVQHAVTALYHMRQRMTAFRRSLPGDLGGGVVFGPVLSGIMGTGEV
                     RREYTVIGDTVNLASRLCDLAMKQAGGGIVIDLATRDLLTEADQGTQHRLTRLNVNSV
                     KGKTRQVDVFNLSDQDFS"
     gene            1230..1493
                     /locus_tag="PKO06_15195"
     CDS             1230..1493
                     /locus_tag="PKO06_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNV71047.1"
                     /translation="MRKKNTTWVVYMVECRDATLYTGITNDLAHRLAMHNAGTGARYT
                     RGRGPVILVFVEPCVNRSDASRRERAIKRLSAPQKRQLINTSH"
     gene            complement(1775..>2441)
                     /locus_tag="PKO06_15200"
     CDS             complement(1775..>2441)
                     /locus_tag="PKO06_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71048.1"
                     /translation="PPIEEYHDDHPDSRESHEEELSTRELFVRNILFNRYLWLFACAN
                     FFVYIARYSMLAWGPTYLKEMKGATMLEGGTSTMLIELAGIPSTLLIGWLSDKWSGRR
                     GMVSLLCMIPIFFAFLGIKLNPPGHLWLDLLLMGIIGFFVYPPVMLLGVAALDLTSKK
                     AVGTAAGFVGLFGYIGSMAQAQGIGYLAQHHGWDSVFNTILACNAAAIVLLALTWNIK
                     PRA"
BASE COUNT          523 a          718 c          709 g          491 t
ORIGIN      
        1 caccacctgc actcctggtt cgggaaccgc atgccagaga cggaccagga agggttgatc
       61 gtttccgagc tggggaacct gcagcgacag taccacccgc cattcgttct cttcgctttt
      121 gatccaatgg attacgggta tggttttacg ccggtgttca ctcccaccga ccccgcgctc
      181 gctgacaccc agtatttttc acggactctt gatgcaccct ttcagagtac tatcggtagc
      241 ggcacgacag aggctttgca cctggttcat ccagtgcagg atcacacgaa agcggtggtt
      301 tcctgtgtgt tgccacttgg tcgcgagcgc ctcctgcagg agtgggaaca tcaactgaac
      361 ctgtggcttg ccggcgcggc gtttctgctc accatcggaa tggcgatgct cgcagcccga
      421 cggtatactg cgcctctgct ggtcttgtct gagggagccc ggctgatcga gagcggcgac
      481 taccgggttc gtctcgcggt cgggcaggat gacgagttcg gcctgctggc gcgccatttc
      541 aacgccatgg cgcatggggt cgaagaaggt cgcctgctga gccggtttgt ttccgaatcc
      601 gttcgcgaag cggcacagag tcgtgacctc gaacaacagg cgcagcgtgg aaccagcgag
      661 gaggtcgtcg tggtgtttgt cggcttgagt accttctcac aactgttgaa ttcgctgccc
      721 cctgaacgcc tgatcgacca tctcaaccgg tatctcgggg tcatggcgcg acagattcgc
      781 gaacagggcg gtcgtatcga caaattcatc ggcgacaaga ttctggcggt cttccgacca
      841 cgcgatttct ccgggccggc cgccgccgtt cagcatgccg tgaccgctct gtaccacatg
      901 cgacaacgca tgacggcgtt ccgtcgttcg ctgcccggag acctgggtgg cggagtggta
      961 ttcggtccgg tcctgtccgg gatcatgggt accggagagg tgcgtcgaga gtatacggtc
     1021 atcggtgaca cggtgaacct ggcttcacgc ctgtgcgatc tggccatgaa gcaggccggc
     1081 ggtggaatcg tgatcgatct ggcgacacgc gatctgctga ccgaagctga tcagggcaca
     1141 cagcatcgac tgacgcgtct caacgtgaat tccgtcaagg gcaaaacccg ccaggtcgac
     1201 gttttcaatc tcagcgacca ggacttttca tgaggaagaa gaacacgacc tgggtggtct
     1261 atatggttga gtgccgtgac gcgaccctct atacgggaat cacgaatgac ctcgcccacc
     1321 gcctcgccat gcacaatgcg ggaaccggcg cgcgctacac gcgtggacgc gggccagtca
     1381 tccttgtctt tgtcgaaccc tgtgtcaatc gcagcgacgc ttccaggcgc gagcgcgcca
     1441 tcaaacgatt gtcagccccc cagaagagac aattgatcaa caccagccac tgacgccgag
     1501 aacgccgtct ctttcctgat cgtacgatga cgttgcgtca agagtcgtcg tcaggtggaa
     1561 acacggccct cgtcacgaaa aagattccgg caacaaagag cgccagctcg ggaacatgat
     1621 tttttctccc actccgagtc gacaaattgt ccagggtatt ttgaacgaga attcccccca
     1681 gaagcatcag aacaagacct atgatgaacg gaaaaagtct acgctgcacg gcggttcacc
     1741 atgagagcca ccgcgaccag tgggcgcggc actttcatgc ccttggcttg atgttccacg
     1801 tcaacgccaa taacacaatg gctgcggcgt tgcaggcgag aatcgtgttg aaaacgctgt
     1861 cccagccatg atgttgggcc aggtatccga ttccctgtgc ctgagccatg ctgccgatgt
     1921 agccaaagag accgacgaat ccggcggccg tgcccacggc tttcttcgaa gtgaggtcga
     1981 gcgcggcaac accgagaagc atcacgggcg gatacacgaa gaagccgatg attcccatca
     2041 ggagcaggtc gagccacaga tggcccggcg ggttcagctt gatgcccaga aaagcgaaaa
     2101 agatcggtat catgcacagc aggctgacca tgccccgcct tcccgaccat ttgtccgaga
     2161 gccatccgat cagaagagtg ctcggaatgc cggcgagctc gatcagcatt gtcgatgtac
     2221 cgccctcgag catcgtggcc cccttcatct ctttcagata ggtcggcccc caggccagca
     2281 tcgaataccg cgcgatatag acgaaaaagt tggcgcaggc aaacagccag agatagcgat
     2341 tgaagagaat gttgcgaacg aagagctcgc gggtcgacaa ctcttcctca tgggattcgc
     2401 ggctatcagg atggtcgtca tgatactctt cgatcggcgg c
//
LOCUS       DAORHD010000770         2440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52398_length_2440_cov_2.425996, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000770 DAORHD010000000
VERSION     DAORHD010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52398_length_2440_cov_2.425996"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKO06_15205"
     CDS             complement(<1..582)
                     /locus_tag="PKO06_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71049.1"
                     /translation="MDLRRLKLLSLGLWKALVLCYWLLCRRDWRNAWSFVYATLFTRD
                     AGLALLDPIYRIFPRLASYPKQIEVETTTACQLKCQICEHSYWNEKPRHMSFEQFLQI
                     MNMFPDLRWIGLTGIGSGFLNPDYRRMLRFLKQEKKVFVEFFDHFSHLEKSWMDEWID
                     LGIDKVWVSLENARAESYEKNRVGASFAKVMENL"
     gene            complement(586..1869)
                     /locus_tag="PKO06_15210"
     CDS             complement(586..1869)
                     /locus_tag="PKO06_15210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV71050.1"
                     /translation="MKSTLLHLTNYFPGYHQHCGGAEQATLRTIQSLVSAGYQNCVAT
                     TPHDFSSGVALPRIRVQHVRTFESLLCWPLVSCVEILKWYSLQFDPLIPKQLDRIHRR
                     QGFSCVLAYNAQFLTAAAYSWASRHRLPLIQFVYDYWLFCPLTTLMQTAGPCRREHGT
                     HCLSCLPSSMRFIQRLFLYLRTIVFRRIRSLIDWWVVLSEASKRLLVEFGIRPERVVV
                     IPVAMNPDWFPVYEGPVDEHRLFFAGWLQQRKGLEVLIQALPKVVEEFPGVWLDVFTM
                     SVKWEAGYEQRVERLVRELGLEARVRLCKTKDVERFRESWKRAAVVVIPEQWENMSPL
                     ILLESQLIGKFVVASRLGGIPEYVAEGVTGLLFDSQDPVDLADRIIQALRGGRQQQIM
                     ARVNGPDMIRERCHPDTVGKKLQQVVLAAQRQGVF"
     gene            complement(1875..>2440)
                     /locus_tag="PKO06_15215"
     CDS             complement(1875..>2440)
                     /locus_tag="PKO06_15215"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71051.1"
                     /translation="NGIRADRFSERQAIAMKKSGCVQVGFGIESVDESVLEGIRKGET
                     FREIERAVQIARQHFEKVAGFFVIGLPGSSLEADLRSLFWAREQGLRAYFSFLVSPEQ
                     TLRGKTFYGVSANPESCAYPMEDQLRVYREAKLLNRRHYEQDHLVWRILTATWQVRHQ
                     YSRRSWVNHLRHLLVRAAGFLRRAELQ"
BASE COUNT          644 a          716 c          569 g          511 t
ORIGIN      
        1 cagattttcc atgaccttcg caaacgatgc ccccacgcga ttcttttcgt atgactctgc
       61 ccgcgcattc tccagagaca cccagacttt gtctatgccc aaatcgatcc actcgtccat
      121 ccaagacttt tccagatgcg aaaagtgatc aaagaactcg acaaagacct tcttttcctg
      181 tttcaggaac cgcaacatcc tccggtagtc aggattgaga aaacccgatc caataccggt
      241 caagccaatc caacgaaggt caggaaacat gttcatgatc tgcagaaact gctcaaacga
      301 catgtggcgt ggcttctcat tccaataact gtgctcgcaa atctggcact tcaactgaca
      361 ggcagtcgtc gtctcaactt caatctgttt cgggtaggac gctaatctcg gaaagatcct
      421 gtagatcgga tcaagcaacg cgagcccggc atcccgggta aacagggtcg cgtagacaaa
      481 ggaccaggca ttccgccaat cccgccgaca gagaagccag tagcacaaca cgagggcttt
      541 ccagagtccg agggacagca gtttcaatct gcgaagatcc ataccttaga acactccttg
      601 tcgttgagcg gccagcacca cttgctgtag cttcttccca accgtatccg ggtggcatcg
      661 ttcccgaatc atatcaggtc cgttgacccg ggccatgatc tgttgttgtc ttcccccacg
      721 cagagcctga atgattctgt ctgccaaatc aacaggatct tgagaatcga acaagagtcc
      781 cgtcacacct tcggccacat actctggaat tccacccaac cgcgaagcca ccacgaattt
      841 cccgatcagt tgactctcca gaagaatcaa aggtgacata ttctcccatt gttctggaat
      901 cacaacgacg gcggcccttt tccaggactc tcgaaagcgc tcgacatctt tcgtcttaca
      961 caagcgcact cgcgcttcaa gacccaactc acgcaccaac cgttcgactc tttgctcata
     1021 ccccgcttcc cacttgaccg acatggtgaa cacatccagc cacacaccgg gaaactcttc
     1081 cactacctta ggcagagctt gaatcaaaac ctccagcccc ttacgctgct gtaaccaccc
     1141 agcgaaaaag agccggtgtt cgtcgactgg cccctcataa acggggaacc agtccggatt
     1201 catggccaca ggaataacaa cgactctctc gggtcgaatc ccaaactcca ccagcaagcg
     1261 tttcgatgcc tctgacagaa caacccacca gtcgatcaaa gaccgaattc gtctaaacac
     1321 gatggtgcgc aaatagagaa acaacctttg aatgaaacgc atggaggagg gaaggcacga
     1381 taagcaatga gtaccatgtt cacggcgaca aggtccagct gtctgcataa gcgttgtcaa
     1441 tggacagaac aaccagtaat catagacaaa ctggatcagc ggcagtctgt gccgagatgc
     1501 ccatgaatac gcagccgccg taagaaactg cgcattgtag gcaagcacac acgagaaccc
     1561 ctgccgtcgg tgaatacggt ccagttgttt cggtatcaga gggtcaaatt gcagagagta
     1621 ccatttgaga atctctacgc aactcaccaa tggccagcag agcagagact caaaagtgcg
     1681 cacgtgctga acccgtattc gtggcaacgc cactccactg gaaaagtcat gcggagtggt
     1741 tgccacacaa ttctggtaac cggccgacac caaactttga atagtgcgca gagttgcctg
     1801 ttcggccccg ccgcaatgtt gatggtaacc ggggaagtag ttcgtcaggt gcagaagcgt
     1861 agatttcaca cgtttcactg cagttcagca cgtcgtaaga agccagcggc gcgcacgaga
     1921 agatgccgaa gatggttgac ccacgatcgc ctggagtact gatgacgcac ctgccacgtg
     1981 gccgtgagga tacgccacac aaggtggtcc tgttcgtagt gtcggcggtt cagaagcttc
     2041 gcctctcggt acacccgcaa ttgatcttcc atcggatacg cacacgattc ggggttcgcg
     2101 gagactccgt aaaatgtctt cccacgaagg gtctgttcag gggaaacgag gaagctgaag
     2161 tatgctcgca gcccttgttc acgagcccag aacaaactcc gcagatccgc ttccaaactg
     2221 ctgccgggaa gtccgatcac gaagaatcca gcgaccttct cgaaatgctg tcgagcaatc
     2281 tgaacagcgc gctcgatttc gcgaaacgtc tcgccttttc tgattccttc gagaacactt
     2341 tcatcaacgg attcaatacc aaatccgact tgcacgcatc cggatttttt catggcaatc
     2401 gcctggcgtt ccgaaaagcg atcggcgcgg atgccgttga
//
LOCUS       DAORHD010000771         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52422_length_2439_cov_2.658138, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000771 DAORHD010000000
VERSION     DAORHD010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52422_length_2439_cov_2.658138"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1245)
                     /locus_tag="PKO06_15220"
     CDS             complement(154..1245)
                     /locus_tag="PKO06_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019592879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNV71052.1"
                     /translation="MVQHDETLKNLFSHPEMVSDLLRGFIREDWVRQLDFSTLEKVNT
                     RFVSERAHSRESDITWKVRWGDGWLFILILIEFQSQPDRWMSLRMLAYVSLLYQDLIK
                     QKQIKAHDRLPPVLPIVLYNGSREWHEPIAFSDLMSESPRGLERFIPRFEYLLVDESR
                     IPLEDVEKSNLAKALFALERSFTVQEVANIVAALGPFFRAPRNRSLMRAFSVWLSRAL
                     LPSHQIEIPPDKLDDFGEVQNMLQERVKQWFKEWKAEGLAEGRLEGRLEGRQEGRQEG
                     RQESRREIALAMLEQGLNLALIAKVTGLTPEEVDQLAEEAAAQRKAVSTPDGNKVSEP
                     SPAPFRAPRRTAKPASKSTPTTRRRRQTT"
     gene            complement(1400..1813)
                     /locus_tag="PKO06_15225"
     CDS             complement(1400..1813)
                     /locus_tag="PKO06_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV71053.1"
                     /translation="MNDRLFVDSNIWIYALIDGAHPHKGDTARRVIAEESIIVLSTQV
                     ISEVCYNLIRKAKAEEALVRSLIQDFFLRYSVVPHTEQMFLRASELRTRYSISYWDSL
                     ILASALLSGCKKLLSEDKQHCLKVDELEIVNPFSA"
     gene            complement(1803..2033)
                     /locus_tag="PKO06_15230"
     CDS             complement(1803..2033)
                     /locus_tag="PKO06_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71054.1"
                     /translation="MTKRIALEFEGIVENGVIHLPPDQKNLNAVKARVILLIDETETT
                     GIPGKGAIRALLEHPLKIPGFTPMKRDEIYER"
     gene            2151..2351
                     /locus_tag="PKO06_15235"
     CDS             2151..2351
                     /locus_tag="PKO06_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71055.1"
                     /translation="MWGETWLKHAGCWPKYQVRLARRDGFRFVYVYLWRLGFLDGRKG
                     FQYACLQAFYEFLINLKLDEQR"
BASE COUNT          493 a          650 c          722 g          574 t
ORIGIN      
        1 gaacgtcagg atgatggtcg tgagtgaaat cgacattagg attgagtgta gccatgttca
       61 atcgcgtttg caaactacga gtttctgatc aacctgaagc tggacgaaca gcgctgaacg
      121 ctgattgcga gcgctggggc tcggtggccg gcattacgtg gtctgtctgc ggcggcgggt
      181 cgtgggcgtg ctcttcgacg caggctttgc ggtgcggcgc ggggcgcgga aaggagcggg
      241 agagggttcg ctgactttgt tcccgtcggg ggtggagacg gcttttctct gcgcggcggc
      301 ttcttcggcc agttggtcga cctcttcagg tgtcaggccg gtgactttgg cgatgagtgc
      361 aagattgagc ccctgctcga gcattgccag ggcaatctca cgccgggact cttgccggcc
      421 ctcttgccgg ccttcttgtc ggccctccag cctgccctcc agccgccctt cggccaggcc
      481 ttcggctttc cactctttga accactgttt gacgcgttct tgtaacatgt tctgaacctc
      541 cccgaaatca tcgagcttgt ccggcggaat ttcgatctga tgtgagggca acagggctcg
      601 actcagccag acggaaaacg cccgcatcag tgatcggttg cggggcgcac ggaaaaacgg
      661 acccagcgcc gccacgatgt tggcgacttc ctgaaccgta aaactgcgtt ccaaggcgaa
      721 aagcgctttg gcaaggttgg acttttccac gtcttcgagt ggaatgcgac tctcatcgac
      781 caacagatac tcaaaccgcg gaatgaagcg ctcaagccca cgcggcgact cggacatcag
      841 atcgctgaag gcgatcggtt catgccattc ccgactgccg ttgtagagca cgatcgggag
      901 aaccggcggc agtcggtcat gagctttgat ctgcttctgc ttgatgagat cctggtagag
      961 cagagaaacg tatgccagca tacgcaggct catccagcga tcgggctgtg actgaaactc
     1021 gatgagaatc aggatgaaga gccagccgtc gccccagcga accttccagg tgatgtcgct
     1081 ttctcgtgaa tgcgcgcgct cagagacaaa ccgggtgttc accttttcga gggtcgagaa
     1141 atcgagctgg cgaacccaat cctcgcggat aaagccgcgc agaagatcgg acaccatttc
     1201 cggatggctg aagaggttct tcagcgtctc atcatgctga accatacacc cagcttaccc
     1261 cgaacggcac cgcgattcaa ggccagatgg ccggcattac ggggtgcgtt tgttgccgcg
     1321 ggatattcgc ggggtcgccg ccggcttcga cgcaggcttc gcggtgcggc gcggggcgcg
     1381 gagcagggta tttcctgatc tatgcggaaa aagggttgac gatctcaagc tcatcaacct
     1441 tcagacagtg ttgcttatcc tcagaaagaa gtttcttgca ccctgacagg agcgcgctcg
     1501 cgaggatcag actgtcccag taagagattg aatagcgagt tcgcagttcc gaggctctca
     1561 gaaacatctg ctctgtgtga ggcacgaccg agtatctgag gaaaaagtcc tgaatcaggc
     1621 ttctgaccaa tgcttcttct gccttggcct tgcgaatcag gttgtaacac acctcagata
     1681 ttacctgggt cgagagaacg atgatgctct cttctgcgat cacgcggcgg gcagtgtctc
     1741 ccttatgtgg gtgcgctccg tcaatcaggg catagatcca gatgtttgaa tcgacaaaca
     1801 gcctatcgtt catagatctc atcgcgtttc attggggtaa aacctggaat tttcaacgga
     1861 tgctcgagga gagctctaat cgcacctttc cccgggatgc ctgttgtctc tgtttcgtca
     1921 atgagaagaa tcacccttgc ttttactgca ttgaggttct tttgatcagg aggaaggtgt
     1981 atgacgccgt tttccacaat tccctcaaac tccaaggcaa ttctctttgt catgttccct
     2041 gtctccagct gatagactcg ttgtgttatt ctactacaaa cagcgagtat tagtgtaaag
     2101 acgcaggctc tctttttctg tttttgacgc tgaatgaaga ggggaagctg atgtggggcg
     2161 agacctggct gaagcacgcg ggatgctggc cgaagtatca ggtgcgactg gcgcgccgcg
     2221 atgggttccg gtttgtctat gtctacctct ggcgtctcgg gtttctcgac ggccgcaagg
     2281 gctttcaata cgcctgcctg caggccttct acgagtttct gatcaacctg aagctggacg
     2341 aacagcgctg aacaacggtt tggggctgta gggcctggtg gccggcatta cggggtctgt
     2401 ctgcggcggc gggtcgtggg cgtgctcttc gacgcaggc
//
LOCUS       DAORHD010000772         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52440_length_2438_cov_4.341167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000772 DAORHD010000000
VERSION     DAORHD010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52440_length_2438_cov_4.341167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PKO06_15240"
     CDS             <1..914
                     /locus_tag="PKO06_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNV71056.1"
                     /translation="ALHELLKRLAELAHPSESGAALSVTLDLQGAKVRIGSFPYEHLT
                     AGPVELHFGWEFRDPRVIPVPFACIFEQTAPGDELLLNDGRVRLRIQSQPEAHTLRAE
                     CVQPGKLSTSKGLNRPSCPYRLSTVSLRDQESIRIGLEFPFVDFAVSFVTSKSEAAIY
                     RPLTGSRRLIAKIERLPALQALSELDQAYDELWLCRGDLGTEVEPYRLGELQAGFARE
                     LKRLRKPCLLAGEVLGSMLYQPVPTRSEMVHLYDALQAGFFGIVLSDETAVGPHALQV
                     VEFLRQFQAAYKLENGEKPNWQEMSAR"
     gene            complement(964..2121)
                     /locus_tag="PKO06_15245"
     CDS             complement(964..2121)
                     /locus_tag="PKO06_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV71057.1"
                     /translation="MPEKPKTRRDIQREASEKAASRKARSGKATPVKAEEPIVTTEPA
                     KPVKARASEKPEEAAPEQSHEKLPLKTGTKPAEPPVAPVDDEEEEVTGPTPEDLEEEE
                     EKEKAESIFDDSVKLYLQQLTKIPLATQQEEVAFAKRIVEGDPDARQALITANLRLVV
                     SIAKKYTNRGLLFLDLIQEGNLGLLRSIEKFDYTMGYKFSTYSTWWIRQAITRALAEQ
                     SRVIRIPVHIMEILNKVKKSIRMFQQENGREPTLDEISARVSLPKERIEEVVKLTQEP
                     VSLEMSVGTKEDSALENFIEDSDAASPEEAVIDSLLKEQIDRVTECLSEKEAQVIALR
                     FGLEDGIPRSLEEIGRIMSVTRERVRQIEEKALKKLRNFSSPILQDFYSRS"
     gene            complement(2114..>2438)
                     /locus_tag="PKO06_15250"
     CDS             complement(2114..>2438)
                     /locus_tag="PKO06_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009226744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HNV71058.1"
                     /translation="YSVYSVISAEGCAAILWKDAAKSKEAAVAMKMRANDLLEFGVID
                     DIIPEPTGGAHRDYDTIAANLKETINRHLPELLALSSDELIERRYRKYRSLGKFKDQQ
                     DMDDA"
BASE COUNT          434 a          674 c          721 g          609 t
ORIGIN      
        1 aggctctgca cgagttgctg aagcgcctgg cggaacttgc tcacccctcg gagagtggtg
       61 cggcactctc ggtgactctt gatctccagg gggcgaaagt ccgaattggc tcgtttcctt
      121 atgaacatct gacggccgga cccgtggaat tgcatttcgg ctgggagttt cgcgatccgc
      181 gtgtgatccc cgtgccgttt gcctgtatct tcgagcagac cgctcccggg gatgaactgc
      241 tgttgaacga tggtcgggtc agactgcgca ttcagtcaca acccgaggcc catacgttac
      301 gtgccgagtg cgttcaaccc ggaaagctga gcacctcgaa aggactcaat cgcccctcgt
      361 gtccgtaccg gttgtcgacc gtatctttgc gtgaccagga gtcgattcgc attggcctgg
      421 agtttccgtt tgtggatttt gccgtctctt tcgtgacctc gaaatcagag gccgccatct
      481 atcggcccct gaccgggtcg cggcggttga tcgccaagat cgagcgattg ccagcactcc
      541 aggctctttc cgagttggac caggcctatg acgaactctg gctctgccgt ggtgatctgg
      601 gcaccgaagt cgaaccgtac cggttgggtg agctgcaggc aggcttcgca agagagttaa
      661 agcgtctacg taagccctgc ctgctggccg gcgaggtttt gggttcgatg ctgtatcaac
      721 cggtaccgac gcgcagcgaa atggtgcatc tgtatgatgc tctgcaggcg ggtttctttg
      781 ggatcgtcct ctcggacgag accgcggtcg ggccacatgc tctgcaggtg gtagagttct
      841 tgagacaatt tcaggcggcg tacaagttag agaacggtga aaagcccaat tggcaagaaa
      901 tgagtgcgcg ttaaaagggt ggctcgcgct gaaatgccgg agttgaggcc gtgggttccg
      961 gtgtcaggag cggctgtaga agtcctgcag gatcggtgaa gagaagttgc gcagcttctt
     1021 gagcgctttc tcttcgatct ggcgcacgcg ctcgcgggtg accgacatga tgcggccgat
     1081 ttcttccaga gagcggggaa tcccgtcttc gaggccgaac cgcagcgcga tcacctgagc
     1141 ttctttttcc gagaggcact cggtcacgcg atcgatctgc tctttcagca gcgaatcgat
     1201 cacggcctct tccgggctgg cggcgtcaga atcttcgatg aaattctcga gcgctgaatc
     1261 ttccttggtg ccgaccgaca tctcgagaga caccggttcc tgggtcagtt tgaccacttc
     1321 ctcgatgcgt tccttgggca gcgacacccg ggccgagatc tcgtcgaggg taggctcgcg
     1381 tccgttctcc tgctggaaca tgcggatcga tttcttgacc ttgttcagga tctccatgat
     1441 atgcacggga atccggatca cccgcgattg ctctgccagc gctcgtgtga tggcctgccg
     1501 aatccaccag gtggaatagg tcgagaattt gtaccccatc gtgtaatcga acttctcgat
     1561 tgaacgcagg agtccgaggt tgccttcctg aatcaggtcg aggaacagca gcccacgatt
     1621 cgtatacttc ttggcaatgc tgaccacgag gcgcagattg gcggtaatca gtgcctgacg
     1681 ggcatcagga tcaccttcga cgattcgctt ggcgaaagca acctcttcct gttgcgtggc
     1741 caacggaatc ttggtcagtt gctgcagata cagtttgacc gagtcatcga agatcgactc
     1801 ggccttttct ttctcttctt cttcttcgag atcttcgggc gttggcccgg tgacttcttc
     1861 ctcttcgtcg tcaacaggcg cgactggcgg ttcagccggc ttggtgccgg tcttgagtgg
     1921 cagcttttcg tgactctgtt ccggagccgc ctcttctggt ttttcagaag cgcgcgcttt
     1981 gaccggcttg gcaggttccg tcgtgacgat gggttcttcc gctttcaccg gggtcgcttt
     2041 tcccgaacgt gctttccgcg aagcggcttt ttcagaggct tcgcgttgaa tgtcccgtct
     2101 cgttttcggc ttctcaggca tcgtccatgt cctgctggtc tttaaacttc cccagcgatc
     2161 gatacttcct atatcgtctt tcgatcagct catccgaaga gagagcgagc aattcaggaa
     2221 gatggcgatt gatggtttct tttaggttcg cggcaatcgt gtcgtaatca cggtgtgcgc
     2281 cgccggtcgg ctcgggaatg atgtcgtcga tgacgccgaa ctcgagcaga tcgttggcgc
     2341 gcatcttcat ggccacggcg gcttctttcg atttggcggc atccttccag agaatggccg
     2401 cgcagccctc ggccgaaatg accgaataga cgctgtat
//
LOCUS       DAORHD010000773         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52450_length_2438_cov_3.198909, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000773 DAORHD010000000
VERSION     DAORHD010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52450_length_2438_cov_3.198909"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKO06_15255"
     CDS             <1..493
                     /locus_tag="PKO06_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71059.1"
                     /translation="ADPVKPPADKPVEPTNPPEIKPNVTTSTEDKNEWAVAHIAVHNR
                     SKEDAGSFVAVTKGDWVVKCWTEKPDFKASEKLIIRLQTNQACKLTELTNIGASGNTN
                     PLLPAGQAIDLKENIINVLPGKDHKFIYEAAAPAGADSVIAKITNDKGESMDLTVKLQ
                     TAN"
     gene            580..1533
                     /locus_tag="PKO06_15260"
     CDS             580..1533
                     /locus_tag="PKO06_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV71060.1"
                     /translation="MKTHRSLFPSGILLLALSLALGGFGTVFADSAEDLMKQALQAES
                     PAAKRAIYEKVLTTNPEHYIAQNNIASTYESEGNLDQAAGWYEKAIRTAEAQGKPYAL
                     AHFGLADVYFARENYSKAWANYQAGLKIDSTDAQALARTNEIKQKFPGYFDSQGNFLL
                     KDSDEIVQAANHPAFRSLSVGEDVEQAMKSGKTRAIVVQPKVQLTILFDYDSDKIRDE
                     SKLQLKNLGQAINAKEMSDFSFYVDGHTDSQGSNDYNQKLSQRRAKAVKDFLSGTMDI
                     TPDRLVERGFGEDKLMAVPEKTPDDQQKNRRVEVMVKGRLQ"
     gene            complement(1625..>2438)
                     /locus_tag="PKO06_15265"
     CDS             complement(1625..>2438)
                     /locus_tag="PKO06_15265"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71061.1"
                     /translation="PWELSCLTRNFSFVPAGMAPAQTPTMSEPPVTQTPITTSEPAPE
                     TTVSVPSTPAPANEPPKESRWDRMWNSIGWGSKSTPAPQTLPEPAQAPASTVSSNQTG
                     DYSLKARAENGDAQAQYELGVRYFEGREVPKDTIEALRWFKMSADGGVASGQIKLGWM
                     YENGKSVAQDLAEARRWYAKAADQGNPFAEKALKNLDAKKAKASAPKKPKPAKKPAMV
                     ANVAAPSAPSAPAVKQTAPAKSSQKEDWKKVRKALEQELIKGLLQGLNKKKR"
BASE COUNT          538 a          713 c          689 g          498 t
ORIGIN      
        1 ggccgacccg gtaaaaccgc cggctgacaa gcctgtcgaa cccacgaatc ctcccgaaat
       61 caagcccaac gtgacgactt cgaccgaaga caagaacgaa tgggcggttg cgcacatcgc
      121 cgttcacaat cgttcgaaag aagatgccgg ctcattcgtc gccgtcacca agggtgactg
      181 ggtcgtgaaa tgctggacgg aaaaaccgga tttcaaagcg tcggaaaagc tgatcattcg
      241 cctgcagacc aatcaggcct gcaagctgac cgagttgacc aacatcggcg ccagcggcaa
      301 caccaacccg cttctgcccg cgggtcaggc catcgatctg aaggaaaaca tcatcaatgt
      361 gcttccgggc aaggatcaca agttcatcta cgaagccgct gctccggccg gcgcagattc
      421 cgtcatcgcc aagatcacaa atgacaaggg cgaaagtatg gatctgaccg taaaattgca
      481 gactgccaac taagacgttc actctgaccg accccgttcg cggggtcggt tggttttccg
      541 gcacctgctg gttgaacccg atcttcagga ggatcacgca tgaagactca tcgttcgctg
      601 tttcccagtg gcattctgct gctggccctg tcactcgctc tcggaggttt cggcaccgtc
      661 ttcgccgact cggccgagga tctgatgaag caggctttgc aggccgaatc gcctgccgca
      721 aagcgagcca tctacgaaaa ggtgcttacc acgaaccccg agcattacat cgcccagaac
      781 aacatcgcct cgacctacga gagcgaagga aatctggatc aggccgccgg atggtacgaa
      841 aaggccattc gtacggcaga agctcagggg aaaccttatg ctctggctca tttcggtctg
      901 gccgatgtct attttgcccg cgagaactac agcaaagcct gggcaaacta tcaggccgga
      961 ttgaagatcg actcaaccga tgcccaggct ctggctcgga caaacgaaat caaacagaag
     1021 tttcccgggt atttcgactc acaagggaac tttctgctga aagattccga tgagatcgtg
     1081 caagcggcga atcacccggc ttttcgcagt ctgtcggtcg gggaggatgt cgagcaggcc
     1141 atgaagagcg gcaagacacg cgccatcgtc gtgcaaccga aagtacaact taccattctc
     1201 tttgattatg acagcgacaa gattcgagac gaatcgaaat tgcagctcaa gaatcttggt
     1261 caggcgatca acgcgaagga gatgagcgat ttttcctttt acgttgacgg gcacacggat
     1321 tcacagggtt cgaacgacta caatcagaag ctgtcacagc gccgagccaa agcggtgaaa
     1381 gactttctga gcggaacgat ggacattacc cctgaccgtc tggttgagcg cggtttcggc
     1441 gaagacaagc tgatggccgt tcccgaaaaa acccccgacg atcagcagaa gaatcgtcgg
     1501 gtcgaggtca tggtgaaggg gcgtctgcag taagcagccc tgccacggca gatcaccgac
     1561 aagcgcctga agaacacgag ggcgaaacca ctcaggtttc gccctcgttc tttcttcgcg
     1621 gcgtctatcg cttcttcttg ttcagaccct gcagcagtcc cttgatcagt tcctgttcga
     1681 gggcttttcg gaccttcttc cagtcttcct tctgtgaact cttggccggc gccgtctgct
     1741 tgacggccgg agcagaagga gccgagggag cggccacgtt cgcgaccatc gccggtttct
     1801 tcgccggttt gggctttttg ggggccgacg ccttcgcctt cttcgcatcc aggttcttca
     1861 gcgccttttc ggcaaacggg tttccctggt cagcggcttt ggcataccag cgacgggctt
     1921 cggcgagatc ctgagcgacg ctcttgccat tctcgtacat ccagccgagc ttgatctggc
     1981 cactggccac accaccatca gccgacattt tgaaccaccg cagcgcttcg atggtatctt
     2041 tcggcacctc gcgcccctcg aagtagcgaa cgccgagttc atactgcgcc tgcgcgtcac
     2101 cgttctccgc gcgcgccttg agagagtagt cgccggtctg gttcgacgaa accgtgctgg
     2161 ccggagcctg agcgggttcg ggcaacgttt gcggagcggg agtcgacttc gagccccagc
     2221 cgatcgaatt ccacatgcgg tcccagcggc tctcttttgg cggttcattc gcaggtgccg
     2281 gagtcgaagg cacggaaacc gtggtttcgg gggccggttc tgaggtcgtg atcggtgtct
     2341 gcgtgactgg cggctcggac atggtcggag tctgcgccgg tgccattccc gccggaacaa
     2401 aggaaaaatt gcgcgtcaag caggaaagct cccatggg
//
LOCUS       DAORHD010000774         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52452_length_2438_cov_3.093580, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000774 DAORHD010000000
VERSION     DAORHD010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52452_length_2438_cov_3.093580"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PKO06_15270"
     CDS             complement(<1..737)
                     /locus_tag="PKO06_15270"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV71062.1"
                     /translation="MTVQATDEQKAGHFAYRVTHESSECAARLGVIETRRGPIHTPVF
                     MPVGTQGTVKTLTPADLREAGAEIILANTYHLMLRPGTAVLDRFGGLHRMNAWDRPML
                     TDSGGFQVFSLKTLNKISDEGVRFASHIDGSRHFLTPEESMRIQRSIGAEIMMCFDEC
                     CSYPATAEQAREAMERSVRWAKRCRVDHPPMRNGQALFGIVQGSMFHDLRRESLERTT
                     EIGFEGLAIGGLSVGEPKELMTETLDRL"
     gene            complement(737..1780)
                     /gene="queA"
                     /locus_tag="PKO06_15275"
     CDS             complement(737..1780)
                     /gene="queA"
                     /locus_tag="PKO06_15275"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNV71063.1"
                     /translation="MKTDLFDYHLPAELIAQVPVQQRPESRLLGYDRRTGTRFHGRFA
                     DLLDLIPRGDLLVLNASKVIPARLFTQPPDPGHRPSEILYLRTAVDGAIEAMIRPGRK
                     FKHGSSHVLPGGCLARVESVRDDGTRVLRICDAVTGRAVADPVEVFRLHGQMPLPPYI
                     TSRESEPARYQTVYAEREGSVAAPTAGLHFDERLLSELEAKGVLIRRLHLHVGLGTFK
                     PIEAENIEDHRLHVEEYEIDDALASTYQEVRRRGGRVWACGTTSVRSLESCVQADDTL
                     RVGRQETGCYLMPGHRFRAVDHLITNFHLPRSSLLVLVSALVGRETLLDLYREAVEQR
                     YRFFSFGDAMVII"
     gene            complement(1777..>2438)
                     /locus_tag="PKO06_15280"
     CDS             complement(1777..>2438)
                     /locus_tag="PKO06_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNV71064.1"
                     /translation="FLPVVRVIDLLGGVEIDVERRMKYTDNWQKLYIDLMPGLQRLDG
                     QKALQYVRFRADAAADLGRIKRQQKFLAALLQQVKSPGIVIKIPDLVRQALNHIQTDL
                     NVAEVVDLARAMLQGPMKINTQSLPGDAPYINGVSYFVPFKDQAVAIGAKGYSSLVSL
                     ELDAGFSLGGASEAQPIPVASAATTTVMLPATGSLPLASDAASASMMTAPVVASGGTR
                     P"
BASE COUNT          511 a          790 c          677 g          460 t
ORIGIN      
        1 agtcggtcga gggtttcggt catcagctcc ttcggctctc ccaccgacaa gccaccgatc
       61 gcgaggccct cgaagccgat ctccgtggtg cgctcgagcg attcccgtcg cagatcgtga
      121 aacatcgacc cctgcacgat gccgaacaaa gcctgcccgt tgcgcatcgg cggatggtcg
      181 acacggcagc gcttcgccca gcgcaccgaa cgttccatcg cctcgcgggc ctgctctgcc
      241 gtcgccggat aggaacagca ctcgtcgaaa cacatcatga tctctgcgcc gatcgagcgt
      301 tgaatcctca tggattcttc cggcgtcaga aagtgtctcg agccgtcgat gtgtgaggca
      361 aaccgcacgc cctcgtcgct gatcttgttc agcgtcttca gcgagaacac ctgaaacccg
      421 cccgaatcgg tcaacatcgg ccgatcccag gcgttcatcc ggtgcaggcc gccaaaacgg
      481 tcgagcacgg ctgttcccgg tctcagcatg aggtgatagg tgttcgcaag aatgatttcc
      541 gcccctgctt cgcgcaggtc agccggggtc agcgtcttca ccgttccctg cgtgccaacc
      601 ggcatgaaca cgggcgtgtg aatcggcccg cgtcgcgttt cgatcacgcc cagacgagcc
      661 gcgcactctg acgactcgtg agtcactcga taggcgaagt ggcctgcctt ctgttcgtct
      721 gtcgcttgta cagtcattag ataatcacca tggcatcacc gaaactgaag aatcggtagc
      781 gttgttcgac cgcctcacgg tacaggtcga gaagcgtctc gcgcccgacc agtgcagaaa
      841 ccagcacgag caggctcgaa cgcggaagat gaaaattcgt gatcagatga tcgacggcgc
      901 gaaagcgatg gccaggcatg aggtaacacc ccgtctcctg ccgaccgacg cgcagcgtgt
      961 catcggcttg cacacacgat tccagcgagc gcaccgaggt ggtaccgcac gcccacaccc
     1021 gaccgccacg gcggcgaacc tcctgatacg tcgaggccag cgcgtcatca atttcatact
     1081 cttcgacatg cagtcgatgg tcctcgatgt tctcggcttc gatcggcttg aaggttccca
     1141 gaccgacatg cagatgcagc cttctgatca gcactccctt cgcttccagt tcggacaaca
     1201 gccgttcatc gaagtgcaac cccgccgtcg gggcggcaac cgaaccttcg cgttcggcat
     1261 acaccgtctg ataccgggcg ggctctgact cacgcgaagt gatatagggg ggcaacggca
     1321 tctgtccgtg cagacgaaac acttccaccg ggtcagccac tgctcgcccg gtcaccgcat
     1381 cgcagatccg cagcacccgc gtgccatcat cgcgtaccga ttcgacccga gccagacacc
     1441 ccccggggag gacatggctc gaaccgtgtt taaacttgcg tccaggccga atcatcgcct
     1501 cgatcgctcc atctaccgct gtacgcagat agagaatctc agacggccga tgacctggat
     1561 ccggtggctg ggtgaacaag cgcgccggaa tcactttcga cgcgttcaag accaacagat
     1621 caccacgcgg aatgaggtcg agcagatcgg caaaacgacc gtgaaaccgc gttcccgtgc
     1681 gtcgatcata accgagcaac ctggactcgg ggcgttgttg aaccggcacc tgcgcaatca
     1741 gttcggcggg aaggtggtaa tcgaagaggt cggtcttcac ggcctcgtcc cccccgaagc
     1801 gaccaccggg gcagtcatca tcgatgccga cgccgcatcc gacgcaagcg gcagcgaccc
     1861 ggtcgctggg agcatgaccg tcgtcgtcgc ggcggacgcg accggtatcg gctgcgcttc
     1921 agaagcccca ccgagcgaga atcccgcatc cagctcgagc gagaccagac tggaatatcc
     1981 tttcgcgcca atagcaaccg cctggtcctt gaacggaacg aagtagctca ccccattgat
     2041 gtagggcgcg tctccaggaa gcgactgggt gttgatcttc atcggtccct gcagcatggc
     2101 ccgggccaga tcgaccacct cggcgacatt cagatcggtc tgaatgtgat tcaacgcctg
     2161 cctgaccaga tcgggaatct tgatgacgat gcctggtgac ttcacctgct gcagcagtgc
     2221 ggcgagaaac ttctgttgcc gtttaatgcg ccccagatca gccgcggcgt ctgcacgaaa
     2281 tcggacatat tgcagtgctt tctgcccatc gagccgttgc agcccgggca tcaggtcgat
     2341 gtacaatttt tgccagttgt ctgtatactt catacgtctc tcgacgtcga tctcaacacc
     2401 accaagcaga tcaatcactc ggacaactgg caaaaatt
//
LOCUS       DAORHD010000775         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52488_length_2437_cov_3.366079, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000775 DAORHD010000000
VERSION     DAORHD010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52488_length_2437_cov_3.366079"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="PKO06_15285"
     CDS             <1..1278
                     /locus_tag="PKO06_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71065.1"
                     /translation="AAATWQAYGETLEKLGQQDLARKAFERALARKKGPVPGRPEPKN
                     ANKYNLGSLKNAAAQDAYRRGAAYLEDHFLLYAIQEFEKALTDEPDNPELIDIAARSM
                     VDYGEEYFSPALDLLKKYRKIKGDAEMGHDRWVALGRAATQAKKYDFALAEEALNSAL
                     KLNAKSLPAQLAMGELKLMRGEYAPAIEWFTKAAEQNKTELRVLWGLGDAHAGLKEHA
                     KALEFYNDAYSQYPQNAEAAYKYAVGLKHLNLLDDSIHFHEVAIGLDPTKARYHLGLV
                     AIYLPRIMDFSAKKHLDAALALEPENPWCYFYYGLYLEMRRRIDEAIEKYEFAKHFGP
                     QMLDVRYQLANIYMGIGNSFPGNNFSGDKPADLVEYMPYKDLKRAYQLYKEIVTINPK
                     YEHAAKIQTQLATVEELYEIQQKLNEEDKRPQR"
     gene            1437..>2437
                     /locus_tag="PKO06_15290"
     CDS             1437..>2437
                     /locus_tag="PKO06_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71066.1"
                     /translation="MVNNRWLSGLVLAALLVLPACFAADVSTIATETRYDVNAAGIQG
                     IKSIPQNEIRRIPDMIDSAKSQIGVWVPMVEPEPIIRGFNNRIADNVEIFKNTGSTKD
                     KYPYIVKVVKNREGKTDQQGFFVYRNVKYDWAFEAAGDDEESGGKNGASFEGKPWTPF
                     YNQGGEEKALEYMRDMAKNGTTEMWDPKAYANVHAKPFDSGISGDADTKGSTVLQEVG
                     MLLTYERASVNVSTSAKGYNGNADLNQIAPGSIPEVQLNAEPSGWQPGKVLNAAPAGW
                     KLEVGGTTQLTAYNRVYVEDYSAPTTKDTEVKVYRGGTGGFVSQVWNKKENKWDPIDG
                     "
BASE COUNT          596 a          636 c          706 g          499 t
ORIGIN      
        1 gccgccgcca cgtggcaggc atatggcgag acgctcgaga agctgggtca gcaggatctg
       61 gctcgcaaag cgtttgaacg tgcgctggct cgcaagaagg ggcctgttcc cggtcgccct
      121 gagccaaaga atgcgaacaa gtacaatctg gggagtctga agaacgccgc cgcgcaggat
      181 gcgtatcgcc gcggagccgc gtatctcgaa gatcattttc tgctgtatgc cattcaggag
      241 ttcgagaagg ctctgacgga tgagcctgac aacccggaat tgatcgatat tgccgctcgt
      301 tcgatggttg attacggcga agagtatttt tcgcccgccc ttgatctgct gaaaaagtat
      361 cgcaagatca aaggtgatgc cgaaatgggc catgatcgct gggtggcgtt gggccgagcc
      421 gccacgcagg cgaagaagta cgactttgct ctggcggaag aagccctcaa tagcgcgctc
      481 aagctcaatg ccaagtctct gccggcgcag ctggcgatgg gtgagctgaa actcatgcgg
      541 ggagagtatg ctccggctat cgaatggttc accaaagccg ccgaacagaa caagacggaa
      601 ctgcgcgtgc tgtgggggtt gggtgatgcg catgccggtc tgaaagaaca cgctaaggct
      661 ctcgagttct ataacgacgc atacagtcag taccctcaga atgcggaagc cgcctacaag
      721 tatgcggttg gtctgaagca cctcaatctg ctcgatgatt ccatccattt tcatgaagtg
      781 gccatcggcc ttgacccgac gaaagcccgt tatcatctcg ggttggtggc tatctacctg
      841 ccgcgcatca tggatttctc ggcgaagaag catctcgatg ccgctctggc gctcgaaccc
      901 gaaaatccgt ggtgctactt ctactacggc ttgtatctgg aaatgcgccg ccggattgac
      961 gaggcgatcg aaaagtatga gtttgccaag cacttcggtc cgcagatgct cgatgttcgc
     1021 taccagctgg cgaacatcta catgggcatc ggcaacagtt ttcccgggaa caacttttcg
     1081 ggcgacaagc ctgccgacct cgtcgaatac atgccctaca aggatctgaa gcgcgcctac
     1141 cagctctaca aggagatcgt gaccatcaat ccgaagtatg agcacgccgc caagattcag
     1201 acgcagctcg caaccgtcga agaactctac gagattcagc agaaactgaa tgaggaagat
     1261 aaacgaccgc aacggtgaaa ttcgtgtaac ggttgtgtta cagtgcccgt aagagagtag
     1321 agaaagtgaa cgttttcgac cacggaaagg agggaggcgg ctgacgcagt ttttttgtgt
     1381 gtcctgaccg aaagagtcct gaatcagaac aaagaaattt ttaccgagga ggaagtatgg
     1441 tgaacaatcg ttggttgtcg ggcctcgtac ttgctgcgtt gctggtactg cccgcgtgtt
     1501 ttgccgcgga cgtgagcact attgctacgg aaacgcgtta tgatgtgaat gcggccggca
     1561 ttcagggaat caagtcgatt ccgcagaacg aaatccgccg cattccggac atgatcgaca
     1621 gcgcgaagtc gcagatcggt gtctgggttc cgatggttga acctgagccg atcattcgtg
     1681 gtttcaacaa ccgcattgcg gacaacgtcg agattttcaa gaacacgggc tcgacgaaag
     1741 acaaatatcc gtacattgtc aaagtcgtga agaatcgcga aggcaagacc gatcagcagg
     1801 gtttcttcgt gtatcgcaac gtcaagtatg attgggcgtt cgaagcggcc ggtgatgatg
     1861 aagaatccgg cggcaagaat ggcgcttcct tcgaaggcaa gccttggacc ccgttctaca
     1921 accagggcgg tgaagaaaag gctctcgaat acatgcgtga tatggccaag aatggcacca
     1981 ccgaaatgtg ggatccgaag gcctacgcca atgtgcatgc caaacccttt gactccggta
     2041 tttccggcga tgccgacacc aagggctcta ccgtactgca ggaagtcggc atgttgctga
     2101 cctacgagcg cgcttcggtc aacgtcagca cctcggcgaa gggatataat ggcaatgccg
     2161 atctgaacca gattgccccc ggctccattc ccgaagtgca gctgaacgcc gaaccttctg
     2221 gctggcagcc cggcaaagtt ctgaacgccg ctcccgcagg ctggaagctc gaagtcggtg
     2281 gcaccaccca gttgaccgcc tataaccgtg tgtatgtcga agactacagc gctccgacca
     2341 ccaaagacac cgaggtgaag gtctatcgcg gcggcaccgg cggtttcgtt tctcaggtct
     2401 ggaacaagaa agagaacaag tgggatccga tcgatgg
//
LOCUS       DAORHD010000776         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52661_length_2432_cov_3.145982, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000776 DAORHD010000000
VERSION     DAORHD010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52661_length_2432_cov_3.145982"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PKO06_15295"
     CDS             <1..720
                     /locus_tag="PKO06_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71067.1"
                     /translation="SSHHQAMIETIAGLVPQPPNLPPTRSITNPLRYVDVGIALCLVI
                     VIVTFSIRWTNNRNPEVFPPEPLAVPTVTDDGALQTVMPRHPGNPIAESCLRQGMLEL
                     GRGNPNVAEAWFLKAQATDPQHPKFTLALERFKRGRREQDTWSAIQEVFNNGDEDRGW
                     QTFVEQSHSRSFFQQWAMPMIRNLEQRQRSASAAAILQTWLKIHPNDAVARSKLERHF
                     LAIGISKARTLHFIGELHRGK"
     gene            710..>2432
                     /locus_tag="PKO06_15300"
     CDS             710..>2432
                     /locus_tag="PKO06_15300"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HNV71068.1"
                     /translation="MANRTRVCFALKTFLLAVFWIATASWTAEAQLRVNTVTSDNTSV
                     SKGQTGIVVHVDVENIGAGSLPLDGVGLIFRMSGFDTSGSYEATLNQPVLPVTLSPGQ
                     RADCVITVSVKTLADSGYCEIDGWARSGVASYSNALLKHTWAVQEPSELILSSVLGPS
                     VVNRGSSGNSVIVDVGRSGEADAWLRGVDLSPLTPSNYLSWSLLSPSLPKNFGRSYWW
                     DKRWSYRQQLTITNQSLSTLPATYEIKVVLKHAPLVTAGKSLANGNDVRVVFDNGTSY
                     QEIPRYCEQEGATTWTVAGTWASDTTVLWFRLQEPLGPAPAATQKYAIYYGNPAAVNP
                     PGNASDVFTFFDTFENGNLGWTTGRTAAGISLPAGYNPYVPWSNGSPTVGPGAAHSPT
                     TAWGVTLTGNYPDSNVATIELNWLRSPAIDLRGKLRPQLTFWDYLNVESPVADYDKGI
                     LSIHRSPPAGTGDASADGYTLDILESSYIREHFNWQKQTYQLKNSLTSEIIYLHLGFA
                     CDWAANDVIGWMVDNFRIRQVADRDPIIGFGVEEPAPVLPTIKAYFDVSVAETAVAGL
                     DTIDAVAT"
BASE COUNT          501 a          778 c          654 g          499 t
ORIGIN      
        1 tcttctcatc atcaagccat gatcgagacc atcgcgggcc ttgtcccgca gccccccaac
       61 ctgccaccga caagaagcat cacgaacccg cttcgttacg tcgacgtcgg catcgctctc
      121 tgtctggtca tcgtcatcgt gacgttcagc attcgctgga caaacaatcg gaacccggaa
      181 gttttcccgc cagagcctct cgctgtcccc accgtgaccg acgatggtgc gcttcaaacg
      241 gtcatgccgc gacacccggg gaatccgatt gccgagtctt gtctgcgcca gggaatgctg
      301 gaactcggtc gaggcaaccc caacgtcgcc gaggcgtggt ttctgaaagc gcaggcaaca
      361 gaccctcagc acccgaaatt tactctggca cttgagcgat tcaagcgtgg tcgtcgcgaa
      421 caggacacct ggagcgcgat tcaagaggtg ttcaacaacg gagatgagga tcgcggctgg
      481 cagacctttg tggaacaaag ccacagccgg tccttttttc aacagtgggc catgcccatg
      541 attcgcaacc tcgagcagcg tcagcgcagc gcgtcggccg cggctatcct gcaaacgtgg
      601 ctgaagattc atccgaacga cgcagtcgct cgcagcaagc tcgagagaca ttttctggca
      661 atcgggattt ccaaagcccg aacccttcat ttcatcggag aattgcaccg tggcaaatag
      721 gactcgagta tgttttgctc tcaagacgtt cctgctcgcg gtgttctgga tagccaccgc
      781 atcgtggacc gctgaagccc aacttcgcgt caacactgtg acttctgaca acacctccgt
      841 gtcgaagggg cagaccggca ttgtggttca tgtcgacgtc gaaaacatcg gcgccggcag
      901 tctgccgctc gacggcgtgg ggctcatctt tcgcatgtcg gggttcgata cttccggcag
      961 ttacgaagca accctgaatc agcctgttct gccggtcacc ttaagtcccg gccaacgcgc
     1021 cgactgcgtc attaccgtct cggtcaagac cctcgcggac tcgggttact gcgaaatcga
     1081 cggttgggcg cgcagcggcg tggcctctta ctcaaacgct ctgctcaagc atacctgggc
     1141 ggtacaagaa ccgtctgaac tcattctcag ttccgtgctg gggccttcgg tcgtcaatcg
     1201 tggcagcagc ggaaactcgg tcattgtcga tgtcggtcgc tctggcgagg cagatgcctg
     1261 gctgcgtggc gtcgatcttt cgccgctcac cccctcgaac tatctctcgt ggtcgctgct
     1321 ctctccctcg ctgcccaaga acttcggccg ttcttactgg tgggacaaac gctggtccta
     1381 ccggcagcag ctcaccatca ccaatcagtc gctgtccacg cttccggcca cctacgagat
     1441 caaggtcgtg ctcaagcacg cgccgctggt cactgcaggc aaatcactcg ccaacgggaa
     1501 tgatgtccgc gtcgttttcg acaatggaac aagctatcag gagattccgc gctactgcga
     1561 acaagagggc gcgacaacct ggacagtcgc cggcacgtgg gccagtgaca cgacggtcct
     1621 ctggtttcgt ctgcaggaac cgctcggccc ggcacccgcg gcgacgcaga aatacgcgat
     1681 ctattacggc aacccagccg cggtgaaccc tccggggaac gcttcggacg tgttcacgtt
     1741 tttcgacacc ttcgagaacg gcaacctcgg ctggacaaca ggcaggacag ctgcgggtat
     1801 ttctctccca gcggggtata acccctatgt tccctggtcc aacggctctc ccaccgtggg
     1861 tcccggtgcg gcacattcac cgacaacagc ctggggcgtc acattgactg gcaactatcc
     1921 ggatagtaac gtcgccacca tcgagttgaa ctggcttcgc agcccggcca tcgatctgcg
     1981 tggtaaactg cggccacaac tgaccttctg ggattacctg aacgtcgaga gccccgtcgc
     2041 cgactacgac aaaggaatcc tcagcattca tcgttcgcct cccgcgggaa ccggcgacgc
     2101 ttcggcagat ggatatacgc tcgacatcct ggaaagctca tacattcgcg aacatttcaa
     2161 ctggcagaaa cagacctatc agctcaagaa ctccctgact tcggaaatca tctatctcca
     2221 tcttgggttc gcctgcgact gggccgctaa cgatgtcatc ggctggatgg tcgacaattt
     2281 ccgcatacgg caggtcgccg atcgtgaccc gatcatcggc tttggcgtcg aggaacccgc
     2341 gccggtctta cccactatca aagcctactt cgacgttagc gtcgccgaaa ccgccgttgc
     2401 cggcttggac acaatcgacg ctgttgccac cg
//
LOCUS       DAORHD010000777         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52724_length_2430_cov_3.355789, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000777 DAORHD010000000
VERSION     DAORHD010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52724_length_2430_cov_3.355789"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="PKO06_15305"
     CDS             <1..1333
                     /locus_tag="PKO06_15305"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNV71069.1"
                     /translation="TILGITRQDEKPFSTIEFDLAAPVQMTVINHEGKLLRLRVAPPL
                     LAPATATRNEIASVFEKMNWVSSDRYTEFVIQGKEPMGRPILATSPAGLPRLSLPYLV
                     AGSKLPLQRGEEIATGLTYYTDRATTKSGQSDVFVLKVRPDTSLVVFPTLANEGISQR
                     EPLSSMVRRYSAIAGINAAYFTPKGEPIGTMIIDRRLVSSPYFNRSVFGINARQEAVF
                     GHPDFSGVLRWQDREIGIDAINTPRSEDNTMVVYTPEFARSTMTSGKDGIELVLAKSR
                     VIGIRDCDSLIPPDGVVVSATGDKAAHLRRIRLGDLVELSYQVASPWNLVNHAVCGGP
                     RLITDGKIDVGQDERFQASIISGRHPRTAVALTYEGDLLFVVVDGRSRRSSGMTLSEL
                     ASYLKKCGSRYAMNLDGGGSSAMWVQDRIVNRPSDGKERPISNGLLIGTKR"
     gene            1305..>2430
                     /locus_tag="PKO06_15310"
     CDS             1305..>2430
                     /locus_tag="PKO06_15310"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HNV71070.1"
                     /translation="MVYSSEQNARVLGVIAFLLLFAGFTASAQAQSDILAIKDVKVGM
                     KGIGKTVIRGRDIETFNVEVLGILSNNKINENLQINGSSILVRVSGKVIDEAGGIAAG
                     MSGSPIFVNGKLIGGLSSGWVMTDHTVGLVTPIEEMLEIWDYPLSASLPDKSKSLYWK
                     TIDGSAIQIGQHRFNGIHQVADEDEAIELARTNQDAVFVEVAAPVVVQGLYDRAASLL
                     KRKLGGKNVEVVAGSPGAGSDTITGPGTIEPGCAIGIQLARGDVNLTTLGTLTYRDGK
                     RILGFAHPFLKKGNAAFLMTGAHIHHSFSSVQMPFKVGAPTEMIGIITQDREKGVAGE
                     LGRFPTLIPVQIEVIDRDLKRNRTINYQVVQDEGVFQSVIE"
BASE COUNT          529 a          701 c          697 g          503 t
ORIGIN      
        1 gacaatactc ggaatcacgc gccaggacga gaagcccttc agcaccatcg agtttgatct
       61 ggcagcaccc gtgcagatga cagtcatcaa tcacgaggga aagcttctgc gcctgcgcgt
      121 tgcaccgcca ttgctggcac cggcgacggc aacccgcaat gaaattgcat ccgtattcga
      181 aaaaatgaac tgggtttctt ccgatcgtta caccgaattt gtcattcagg ggaaggagcc
      241 catggggcgg ccgatcctgg cgacctcacc cgccgggtta ccccgtctct cgcttccgta
      301 tctggttgcc ggcagcaagc tccccctgca gcgtggagaa gagattgcca ccggactcac
      361 ctactacacc gatcgcgcca ccacaaaatc cggccagtcg gatgtcttcg tgctgaaagt
      421 gcgccccgac acctcgctcg tggtgtttcc cactctggcg aacgaaggta tctcgcagcg
      481 tgaaccgctg tcgtcgatgg tgcggcgcta cagtgcgatt gccggaatca atgcggcata
      541 tttcaccccc aagggtgagc ccatcggaac catgatcatc gatcgtcgac tggtcagctc
      601 gccgtatttc aaccgttcgg tgttcggcat caacgcccgc caggaagccg ttttcggaca
      661 cccggacttt tcgggcgtgc tgcgctggca ggatcgtgag atcggcattg atgccatcaa
      721 tacgcctcgt tctgaagaca acacgatggt ggtctataca cccgagtttg cccgcagcac
      781 gatgaccagc ggcaaagacg gcattgaact cgttctcgcc aagagccgcg tgatcggcat
      841 tcgcgactgc gactctctga ttccgcctga tggcgtcgtg gtctcagcca ccggcgacaa
      901 agcggcacat ctgcgccgca tcagactggg cgatctcgtc gaactcagct atcaggtcgc
      961 cagcccgtgg aacctggtca accatgcggt ctgcggcggt ccgcggttga tcacggatgg
     1021 caagatcgat gtcggtcagg atgaacgatt tcaagcttcg atcatttcgg gtcggcaccc
     1081 gcgaacagcg gtggctctga cctatgaagg cgatctgctg ttcgtcgttg tcgacggccg
     1141 atcgcgccgc agcagcggca tgaccctgtc agaactggct tcgtacctca agaagtgcgg
     1201 atcgaggtat gccatgaatc tggatggagg gggatcctcg gccatgtggg tacaggatcg
     1261 catcgtcaat cgcccctctg acggaaagga aaggcccatc tcaaatggtc tactcatcgg
     1321 aacaaaacgc tagggttctc ggggtcatcg cttttctgct gctgtttgcc ggctttaccg
     1381 cttcggcaca ggcacagtct gacattcttg cgatcaagga tgtcaaagtc ggcatgaagg
     1441 gcatcggcaa gacggtgatc cgcggccgcg acatcgaaac cttcaacgtc gaagtgctgg
     1501 gcattctttc caacaacaag atcaacgaaa acctgcagat taacggcagt tccattctgg
     1561 tgcgcgtctc agggaaagtc atcgacgagg cgggcggcat tgctgccggt atgagcggca
     1621 gcccgatctt cgtcaacgga aaactgatcg ggggactcag ttcgggatgg gtcatgaccg
     1681 accacactgt gggcttggtg acgccgatcg aagagatgct ggaaatctgg gattaccctc
     1741 tcagcgccag cctgccggac aagtccaagt cactctattg gaaaacgatc gatggatcgg
     1801 cgattcagat tggtcaacac cggttcaatg gaattcatca agtagcagac gaagatgaag
     1861 cgatcgaact ggcaagaacc aaccaggacg cggtcttcgt tgaggttgcc gcgccggtgg
     1921 ttgttcaggg cctgtatgat cgagccgctt cactgctcaa gcgaaaactc gggggcaaga
     1981 acgtcgaggt tgtggccggc tcccccggag cgggttccga cacgattacc ggccctggga
     2041 caatcgaacc aggctgtgcg atcggaattc aactggcccg tggtgatgtg aacctgacca
     2101 cgctgggaac cctcacttat cgtgatggca agcgcatcct cggttttgcg cacccgttcc
     2161 tgaagaaggg aaacgcggct tttctgatga ccggcgcgca cattcaccat tccttttcga
     2221 gcgttcaaat gcctttcaag gtcggtgctc cgaccgagat gatcggcatc atcacccagg
     2281 atcgcgagaa gggcgttgcc ggtgaactcg gacgctttcc cactctgata ccggtccaga
     2341 tagaagtcat cgatcgcgat ctcaaacgca atcgcaccat caactatcag gttgtccagg
     2401 acgagggcgt gttccagagc gtgatcgagt
//
LOCUS       DAORHD010000778         2428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52796_length_2428_cov_3.660767, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000778 DAORHD010000000
VERSION     DAORHD010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52796_length_2428_cov_3.660767"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..>2428)
                     /locus_tag="PKO06_15315"
     CDS             complement(35..>2428)
                     /locus_tag="PKO06_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV71071.1"
                     /translation="DTVSNTITMTPVANLDFNQVYTVIASGLLDLVGNPQPQSLIATF
                     TTQTAVPTLPVTINNLLLFADAAYSVPVASGATVLPGSTLYLEIDAADLAPATVDITR
                     VVVTSPSGASVTVVLSETGLSTGIFHGSVALFDEENRVLQVYSETDTTKRKNVILPLK
                     PVYVTLSPASASVDLPLDTTFVVQTNKPYAAASITSSTVHLIDSTGLLTPVITFNGPQ
                     EFRVAAPLATFSEVILRLESGILDTDGLGFAFLRASFTTLLPQLTTCTVFSDDGYTNV
                     LSDGAKVLPGTTTVYVQAQGNDPRPSAVDIIALLVTDGVSTTTQTLTEDLPGRFRGSI
                     SIPNSSNAVIVLTPPLTPGLRRQLVTPPAFAVAAVSPASGAIEVPADSWPRWDFATPL
                     DATQILTGRFTLTLLPATNANGVLELSPDRRTVTFRPDFHLALLKDYELRVNSAVKDE
                     DGRTLGQDFVTRFVSQSPPPPPVSVLTLKNFADALFASESLFLGPESTMYLEVRAVDT
                     SSASVDTTRVRVDSSDGTLVSLELTLLETGANTGLFRLAYPVVLAPGSTVRVVSQADA
                     DFEMNLQVRTRPTLVSVNPASGSVALTLDRRLALNFSRSMSAPTLQVGIEVVGSGGIP
                     VGVTGSLQPDLRTWLLEPVAQWATGTLHTARLKNTIRDSDGVALATVAFRFTTRAIGP
                     ATFDLVTGLPPRDQERISVWHEAVPGPLRLIATTTSLFDGGLEQHPVRLIWSGGTVLA
                     TVTELATAPGLFFGQVDPGLARGVVATATLEYSNQPHQWFRIADLPVLFARSPSGVS"
BASE COUNT          567 a          728 c          688 g          445 t
ORIGIN      
        1 gcatgcacga actcatcctt cggctgccaa agggttatga cacgccgctg ggcgaacgag
       61 cgaacaacac aggcaggtcc gctatccgaa accactggtg tggctgattg gaatactcga
      121 gcgtggccgt cgccacgacc ccgcgcgcca gtcctggatc tacctgtcca aagaaaaggc
      181 ccggggcggt cgccagttcg gtcaccgtgg ccagaaccgt cccacccgac cagatcaggc
      241 gaaccggatg ctgttccaga ccaccatcaa acagactggt cgtggtggca atcaaacgca
      301 gaggccccgg aaccgcttca tgccacaccg atatccgctc ctgatcgcgc ggaggcaacc
      361 cggtcaccag gtcaaaagtc gccggaccta tagccctggt cgtaaaacga aacgccacgg
      421 tcgccagcgc gacaccgtca ctgtcacgaa tggtgttttt cagtcgcgcg gtatgaagag
      481 taccggtcgc ccattgggca acaggctcca gaagccatgt tcgaagatct ggttgtaaag
      541 atccggtcac gccaacgggt atgccacccg aacccacgac ctcgattccc acctgcaacg
      601 tcggagcaga catcgatcgg gagaagttca gagccagacg tctatcgagc gtcagagcaa
      661 cagatccgga tgccggattc acggatacca gtgtcggcct ggttcgaacc tggagattca
      721 tctcaaaatc agcgtctgcc tgcgacacga ctcgtaccgt cgaaccaggt gccagcacaa
      781 ccggataggc cagccggaag aggccggtgt tcgcgcccgt ctccagcagc gtcaattcca
      841 gtgaaaccag agttccatcg ctcgaatcga ccctgactcg cgtcgtgtca accgatgccg
      901 atgacgtatc gacggcgcgt acttccagat acatggtgct ttcggggccg agaaacagac
      961 tttcgctggc aaacagcgca tcagcaaagt tcttcagcgt gagcaccgac accggcggag
     1021 gtgggggcga ctgactgaca aaacgcgtaa cgaaatcctg cccaagggta cggccgtcct
     1081 catctttcac ggcggagttc acccgcaact catagtcttt cagcaatgcc agatgaaagt
     1141 ccggtcggaa tgtcacggtg cgccggtctg gcgacagctc cagaacgcca ttcgcgtttg
     1201 ttgccggcag aagcgtcagg gtgaagcggc ctgtcagaat ctgcgtcgca tccagtggcg
     1261 tcgcaaaatc ccagcgtggc caggaatcgg caggcacctc aatggcccca ctcgctggcg
     1321 atactgcggc tacggcaaaa gcgggtggcg tcaccagctg tcgacgcagc cccggtgtca
     1381 acggcggtgt caaaacaatc accgcatttg aagaattcgg aatgctgata ctgcctcgaa
     1441 atcggccggg cagatcttcc gtcagcgtct gggtagttgt cgacactccg tctgtcacca
     1501 gcagcgcgat gatgtccaca gcgctcgggc gcggatcatt gccttgcgcc tgaacataga
     1561 ccgtcgtcgt ccccggcaac actttggctc catcagacaa cacatttgta tacccgtcat
     1621 ccgagaaaac agtacaagtt gttaactgag gcaagagcgt tgtgaaggag gctcgcagaa
     1681 acgcaaatcc taaaccgtcg gtatcgagaa taccgctttc gagcctcagg atcacttcgc
     1741 tgaaggtcgc caacggagcc gctacgcgaa actcctgcgg accgttgaac gtgatgaccg
     1801 gcgtcaacaa tccggtcgaa tcgatcagat gcaccgtcga tgaggtgatc gaagcggccg
     1861 catacggttt gttggtctga acgacgaagg tcgtgtcgag cggcaaatca acagaagcgg
     1921 aagccggaga caaggtcaca tagaccggtt tcagcgggag aatcacgttc ttgcgtttcg
     1981 tcgtgtcggt ctccgaatac acctgcagaa cccggttctc ttcatcgaac agcgccacac
     2041 tgccgtgaaa aatcccggtc gacaggccgg tttcagacaa gaccaccgtc acgctggctc
     2101 ccgacggaga cgtgaccacc acccgtgtaa tatcgaccgt tgccggcgcc aggtcagcgg
     2161 catcaatctc gagatacagc gtagatcctg gcagcaccgt cgcaccgctg gcgacgggga
     2221 cgctgtacgc ggcatcggca aagaggagca gattattgat ggtcaccggt aacgtcggta
     2281 cggcggtctg cgtcgtgaag gtcgctatga gagactgcgg ttgcgggttc cccaccagat
     2341 ccagcaaacc gctggcaata accgtataca cctgattgaa gtccagattg gctaccggtg
     2401 tcatcgtgat tgtgttactg acggtatc
//
LOCUS       DAORHD010000779         2426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52885_length_2426_cov_2.943484, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000779 DAORHD010000000
VERSION     DAORHD010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52885_length_2426_cov_2.943484"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKO06_15320"
     CDS             complement(<1..202)
                     /locus_tag="PKO06_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71072.1"
                     /translation="MTIQPVVRTQPKYPGSTAKRTLLTAFRSLMTSRVTLTGCLWFLL
                     QCSLVFTSGPVSAQPASPGTLSD"
     gene            401..1489
                     /gene="ychF"
                     /locus_tag="PKO06_15325"
     CDS             401..1489
                     /gene="ychF"
                     /locus_tag="PKO06_15325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNV71073.1"
                     /translation="MKIGLAGLPMSGKTTVFNLCTRLQAQTRDYLAQSEEVNTGVIKV
                     PDERIDHLVSVYKPKSTVYATVEFVDIPGVSKGDAGLSSKTLANIRVCESLGLVVRLF
                     DSDDVPHIRNRVDPASDIDELRLEFIFSDLGIVQNKIERLQREMKGTKKPIQIKEMEL
                     MEKCKTALEANRFLSELTFDTDEEALLKGFRFLTQKPLLVIGNCSDAQLKNAAHPVVV
                     AFQKACADGKLSSMLISAKTEMEISSLSPEEEATFLQEYGITESGRSRLIKQAYGILN
                     YISFLTAGEDECRAWPIQRGTIALKAAGKIHSDIERGFIRAEVVAFDDFVAHKGMAGA
                     KAAGLIRLEGKEYPVADGDIINFRFNVG"
     gene            1493..>2426
                     /locus_tag="PKO06_15330"
     CDS             1493..>2426
                     /locus_tag="PKO06_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71074.1"
                     /translation="MLSGQSESTIGRVRAWTLRRQLRLGIGAGLAFLLLFFLVAGPAW
                     KALESFVQNDLKPDLALAQAYDRVMTEWVRVHKAIPELPDQLLRQSTGVGRVDELASC
                     VRALDATFERAGPGRSRERYLEVSAYIASYSNTLQSLQKALDRRRRLGQEENRKRSEV
                     SQTLVSRTQELVQGFKPMVDDFVKLMKSAEFLDRQAGASEFVLKISRIEKDLVIIQSD
                     LNSYIQLMESQRDIDSQPMTQRILKRLNAVLALLERSIAETQSAAQRRALDRVRSSLA
                     GIKSAFAAVRDLGEKADSDVLELDEEIAQLRRVLT"
BASE COUNT          538 a          654 c          712 g          522 t
ORIGIN      
        1 tgtcactgag agtccctgga gaggccggtt gcgcgctcac tggcccgctg gtgaacacca
       61 gagagcactg caggagaaac cacagacaac ccgtgagtgt aacgcgcgaa gtcatcagcg
      121 agcgaaatgc ggtcaaaagt gtgcgctttg ccgtcgaccc cggatacttc ggctgtgttc
      181 ggacaacggg ttgaatggtc atgctgcccc cctcgcggaa aatcttgcct cttattgtaa
      241 cataagtgaa aacgtttcct gacagcgacc agattgcttt ggaccggtgg gtcgtcacaa
      301 atacctgaga ctgatacgcg gggcatcttt cattccggcg tctggggcgg tataatccag
      361 cgattcgcat ctcatcgccc atacgctgca ggaggacccc atgaagatcg ggctggctgg
      421 tctgccgatg tcaggtaaaa cgactgtgtt caatctgtgc acgcgccttc aggcgcagac
      481 gcgtgattat ctcgctcaga gcgaagaagt gaatacgggt gtcatcaagg ttcccgacga
      541 acgaatcgat catctcgtca gtgtctacaa accaaagagc actgtgtatg ccacggttga
      601 gttcgtcgac attcccgggg tgtcgaaagg ggatgccggc ctgtcgagca aaacgctcgc
      661 caatattcgt gtctgcgaat cgctcgggct tgtcgtgcgg ttgttcgaca gcgatgatgt
      721 gccgcacatt cggaatcggg tcgacccggc gtctgacatc gatgaattgc gtctcgagtt
      781 cattttttcc gatctgggca tcgtgcagaa caagattgag cgtctgcaga gagagatgaa
      841 aggcacgaaa aagcccattc agatcaaaga aatggaactg atggaaaagt gcaagaccgc
      901 gctcgaagcg aaccgctttc tgtccgaact gaccttcgat actgacgaag aggctctgct
      961 caaagggttt cgatttctga cccagaagcc gctgctggtc atcggcaact gctctgatgc
     1021 gcagctgaag aatgcggcgc atcccgtcgt tgtcgctttt cagaaagcct gtgctgacgg
     1081 caaactttcc tcgatgctga tttccgcgaa gacggaaatg gagatctcat cgctttctcc
     1141 ggaagaggaa gcgaccttcc tgcaggaata tgggatcacc gaatccggtc gcagccggtt
     1201 gatcaagcag gcctacggca tcctgaacta catttccttt ttgaccgccg gtgaagatga
     1261 atgtcgggcc tggccgattc agcggggaac catcgcgctc aaggctgccg ggaagatcca
     1321 ttcagacatc gagcgcgggt tcattcgcgc ggaagtggtc gcctttgacg actttgtcgc
     1381 gcacaagggg atggccggtg cgaaagcggc cgggttgatt cgtcttgaag gcaaggaata
     1441 cccggtggcg gacggagaca tcatcaactt tcgcttcaac gtcggctgag gaatgctgtc
     1501 ggggcagtca gaatcgacga ttggccgagt tcgagcctgg acgctgcgaa gacagctgcg
     1561 tctgggaatc ggagccggtc tcgcgtttct gttgctcttc tttctggtgg cggggccggc
     1621 ctggaaagcg ctcgaaagct tcgttcagaa tgacctgaag cccgatctcg ccctggcaca
     1681 ggcatacgat cgggtcatga ccgagtgggt gcgggtgcac aaggcgattc ccgaactgcc
     1741 tgaccagctg ctgcgccagt cgacaggcgt cggtcgtgta gacgaacttg cctcctgcgt
     1801 gcgtgctctc gatgccactt tcgaacgagc cggaccggga cgctcgcgcg aacgctatct
     1861 cgaagtttcc gcgtacattg ccagctattc caataccctg caatctctgc agaaagcgct
     1921 tgatcgacgt cgacgactcg gacaggaaga gaatcgcaaa cgttcggaag tgtcgcagac
     1981 actggtgtct cggactcagg aactggttca gggtttcaag cccatggtcg atgacttcgt
     2041 caagctcatg aagagcgcgg agtttctgga ccgacaggct ggcgcctcgg aatttgttct
     2101 gaagatatca cgtattgaga aagatctggt cattatccag agtgatctca actcgtacat
     2161 tcaactcatg gaatctcagc gcgacatcga ttcgcagccg atgacccagc gcattctgaa
     2221 acgactgaac gctgtgctgg ccctgttgga acgatcaatc gccgaaaccc agtctgccgc
     2281 gcaacgacgc gccctcgatc gggttcgcag cagccttgcc ggtatcaaga gcgcgtttgc
     2341 cgccgtccgt gacctgggcg agaaggcaga cagtgacgta ctcgaactcg atgaggaaat
     2401 cgcccagctg cgtcgtgtct tgacgc
//
LOCUS       DAORHD010000780         2425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52904_length_2425_cov_3.572574, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000780 DAORHD010000000
VERSION     DAORHD010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52904_length_2425_cov_3.572574"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PKO06_15335"
     CDS             complement(<1..430)
                     /locus_tag="PKO06_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71075.1"
                     /translation="MNKLFTTTLRPSTVLRVLVLAVGLLFSSVGLAQPTASEPDELVI
                     LNSYHYGYRWSDGEARGVYEELIAAFPRAQVFHEFLGTKHVPYVESNRAYFDLLAEQL
                     RAKFRSGGTDLIVALDNDALAFLNHYAEKIWPRVPVVFCGV"
     gene            complement(447..1892)
                     /locus_tag="PKO06_15340"
     CDS             complement(447..1892)
                     /locus_tag="PKO06_15340"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV71076.1"
                     /translation="MNLNEFLRGVSLFSMLPDEDLKKISERAQEIKYVRDAFICKEAE
                     KADSMFIIKSGIVQIFCDDGKGGRKILTHLKLGDYFGEMALLTDEPRTASAIALAETE
                     VLRLRKDDFQSLVRGLPDVAFGIIRTLCDRLAKANVGTSSEKKVNVYSVMAPDGSSGK
                     SYLARNLALAMQNRLQKPVLLFDPNVRDDRVARMLGVEQHSRIIDELVERERIGNIQN
                     YVVKAPCGLMTILPQENGFTDLRLKEFHTFSLMKTVFENFEFVVVDSSSMFTKVTKEI
                     VTTADKIIYMISSKNVSVESLVMHFEETRRGWRVDPSRVVYGVNHNIGDTSKEGVITA
                     KAREWLSFELPFENKLVGYREPDKQVLIQREPSHPHSQLVIGLADDILYDQKICLYLP
                     AFPQDATKAELARRWVESAKNELASHLVGISTKESVEKDGKPCVQMIGKAPKWSLNQH
                     VVAVVDFANRYKQEFALEKLLISINGQESVI"
     gene            complement(2017..>2425)
                     /locus_tag="PKO06_15345"
     CDS             complement(2017..>2425)
                     /locus_tag="PKO06_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HNV71077.1"
                     /translation="KLSGPLLDRIDLQIPVPRLKPEELAGLATGESSAVVARRVLEAR
                     QRQAARQARQGLVVLNAHLSPRELRQHAELDADSQKLLTDAARAFGLSARAFDKIIRV
                     ARTIADLDAVDRIASQHVAEAIQYRTIDPFATA"
BASE COUNT          504 a          688 c          703 g          530 t
ORIGIN      
        1 cgaccccgca gaagacgacc ggaacccgtg gccagatctt ctcggcataa tgattcagaa
       61 acgccagcgc gtcgttgtcc agggcgacga tcagatccgt tcctccggat cggaacttgg
      121 cgcgtaactg ctcggccaga agatcaaagt aggcgcgatt cgactcgaca tagggcacat
      181 gcttcgtgcc gagaaactcg tgaaacacct gtgcccgcgg aaatgcggcg atcagctcct
      241 catagactcc gcgcgcttct ccgtccgacc agcgataccc gtagtgatac gaattcagga
      301 tcaccaattc gtcgggttcg gaagctgtcg gctgcgcgag gccgacggag gaaaaaagaa
      361 gcccgaccgc cagaaccagt acccggagta cggtcgacgg tcggagcgtc gtcgtgaaaa
      421 gcttgttcaa cggctcgacg gaaaaatcag atcacgcttt cctggccgtt gatcgaaatc
      481 agcagttttt ccagtgcgaa ttcctgtttg taccggttgg caaagtcgac gacagcgacc
      541 acatgctgat tcaggctcca ctttggagct ttgccgatca tctgaacgca gggtttgccg
      601 tctttttcga cactttcctt tgtggagatg cccaccaggt gagacgccag ctcgtttttc
      661 gccgattcca cccaccgtcg tgcgagttcg gccttggtgg cgtcttgcgg gaacgctggc
      721 aggtacaggc agatcttctg gtcgtagaga atgtcgtcgg ccagaccgat caccagctgg
      781 ctgtgcggat gcgaaggttc gcgctggatc aacacctgct tgtcgggctc gcgataccca
      841 accagcttgt tttcgaacgg aagttcaaag gaaagccact cgcgggcctt ggccgtaatg
      901 accccttctt tcgaggtatc gccgatgtta tggttgactc cgtagacgac ccggctcggg
      961 tcgacccgcc aaccccgccg cgtctcttca aagtgcatga ccaggctttc gaccgacacg
     1021 tttttgctgg agatcatgta gatgatcttg tcggcggtcg tgacgatctc tttcgtcacc
     1081 ttggtaaaca tcgaactgct gtcgaccacg acgaactcga agttttcgaa caccgtcttc
     1141 atcagcgaga aggtgtgaaa ctccttcaga cgcagatcgg tgaagccgtt ttcctgcggc
     1201 agaatcgtca tcagaccgca cggagctttc acgacgtagt tctgaatgtt gccgatgcgc
     1261 tcgcgttcga ccagttcgtc gataatgcgt gaatgctgtt cgacgcccag catccgggcc
     1321 acgcgatcgt cacgtacatt tggatcaaac aatagtaccg gtttctgcag acgattctgc
     1381 atggccagag ccagattccg ggccaggtaa ctcttgccgg aagaaccgtc gggggccatg
     1441 acgctgtaca cgttgacctt cttttcgctc gaggttccga cgttcgcctt ggccagacga
     1501 tcgcagagtg tgcgaatgat gccgaatgcc acatcgggta agccgcgaac caatgactga
     1561 aaatcatctt ttcgcagtcg cagcacttca gtctcggcca gagcgatagc gctggccgtg
     1621 cgtggttcgt cggtcagcag ggccatctcg ccaaagtaat cgcccagctt gaggtgggtc
     1681 agaatcttgc ggccgccttt tccgtcgtcg caaaagatct gaacaatgcc ggacttgatg
     1741 atgaacatcg aatccgcttt ttccgcttct ttgcagatga aggcatcgcg cacgtatttg
     1801 atttcctggg cacgctccga aatctttttc agatcttcat cagggagcat ggaaaagaga
     1861 gagacgccgc gtagaaattc attcagattc ataacttccc ctcaggctgt gtggtttcag
     1921 acttgaccat gctatccccc cggtatcacg gtgtcaacgc ttcgtttcag agtgtcagga
     1981 gagagtggcg gtgcggatac cgaaaggagt cggcagtcac gcggtcgcga aggggtcgat
     2041 ggtgcggtat tgaatcgctt cggccacgtg ttgggaagcg atgcgatcga cggcatcaag
     2101 atcggcgatg gttcgcgcca cgcgaatgat cttgtcgaag gcgcgcgcgg aaaggccgaa
     2161 ggcgcgggcg gcgtcggtca gcaacttctg tgagtcggca tcgagctcgg catgctggcg
     2221 aagttcgcgc ggcgacagat gcgcattcaa cacgaccagg ccctggcgcg cttgtcgggc
     2281 ggcctgtcgc tgacgcgcct ccagaacccg tcgggcgacg accgcggacg attcgcccgt
     2341 ggccagccct gccagttctt cgggcttcag acgtggcacc ggaatctgca gatcgatgcg
     2401 gtcgagcagc ggccccgaaa gtttc
//
LOCUS       DAORHD010000781         2423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_52968_length_2423_cov_3.249155, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000781 DAORHD010000000
VERSION     DAORHD010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52968_length_2423_cov_3.249155"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2083)
                     /locus_tag="PKO06_15350"
     CDS             complement(<1..2083)
                     /locus_tag="PKO06_15350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71078.1"
                     /translation="MGSKSKDTPSFTTRFRSAIVLFGFSVGLILLVFKPFFGWLDTRL
                     RDQWFRTQIHASNPTEFPWLAPIAWLLPPAAQPKDIVLAVIDDRSVLGTPGLFQSDRR
                     AYARLLRLIGEGNPSVIGLDVFFPGTASHDEAADKELAETVQQLGNVVLKAYRRGDQQ
                     MTPPFPALARSGIVAPTYFRPHVDEAVRTCSLFFQPTRGDAVLSFQAQLAKRHLRLDS
                     TSFRVEPGGLTCLIGSETRQFSLVDSEHGLISYHLDPRMIPLVSAHDILMQTVSPTLF
                     TGKIVIVGIATSMSEERYFTPLGRPEFSPFLHGLIVQNLITGQLLSPAVPFLGTILAL
                     ALLAGWLLLAVPRLSPYGIAFLTFLTTIGLLGVSSIGLWKLHLVFEVTGAIFVVVATT
                     VFAIGLRYYAERSEKHRIKNAFQHYVTASVVNEILKNPEKLNLHGEERFLTIFFSDIE
                     GFTTLSEGMSPLSVVALLNEYLTQMTEIIFKFDGLLDKYEGDAIMSVFGAPVDQRDHA
                     IRACRCALEHQKALERLRAKWKSEGKPEIRVRIGINSGNVVVGNMGSEMRFDYTVIGD
                     NVNLAARLESANKLFHSDILISSQTAELVEGAILTRFLGMLRVVGRRQPVQVYEVLMS
                     LDHADPELLNRQQAAKQAYEKALGETSQRRFSDALATLRAHLEQAPTDLPARMLLKRV
                     ESWLSAPPPDDW"
     gene            complement(2192..>2423)
                     /locus_tag="PKO06_15355"
     CDS             complement(2192..>2423)
                     /locus_tag="PKO06_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71079.1"
                     /translation="APLADLEEADNLLVEGKQIGWNIQNGYGAYTGTPGYEPYPIGTR
                     PPRFDPGDRDGPDDGGDDRDGDDRRDDGPRKF"
BASE COUNT          550 a          639 c          738 g          496 t
ORIGIN      
        1 cccagtcgtc ggggggagga gcgctcaacc agctttcgac acgcttcagc agcatacggg
       61 ccggcaggtc tgtcggtgcc tgttcgagat gcgctctgag cgtcgccagc gcatcggaaa
      121 aacggcgctg tgaggtttca ccaagtgctt tttcataagc ctgtttggcg gcctgttgcc
      181 tgttcagaag ttcaggatct gcatggtcga gactcatcaa cacctcgtag acctgcacgg
      241 gctggcgtct gccgaccaca cgcagcatac cgagaaacct ggtcaagatg gctccttcga
      301 ccagttcggc ggtctggctg ctgatcaaaa tatccgaatg gaacagcttg ttggcggact
      361 caagacgcgc ggcgagattg acgttgtcgc caatgacggt gtagtcgaag cgcatttccg
      421 aacccatatt tccgaccacg acgttcccgg agttgatgcc gattcgcact cggatttccg
      481 gcttgccttc gctcttccat ttcgcgcgca ggcgctcgag cgctttctga tgctcaagcg
      541 cgcagcgaca ggcgcgaatg gcgtgatcac gctggtcgac cggcgcgccg aagacggaca
      601 tgatggcgtc gccctcgtac ttgtccagaa gcccgtcgaa cttgaagatg atttcggtca
      661 tctgggtcag atactcgttt aacagtgcga ccacgctgag tgggctcata ccttcagaaa
      721 gagtcgtgaa gccctcgatg tccgagaaga agatggtcag aaaacgctct tcgccgtgca
      781 ggttcagctt ttccgggttt ttcagaattt cgttgacgac cgaggcggtg acatagtgct
      841 gaaacgcgtt cttgatgcgg tgtttctcgc ttcgctcggc atagtaccgc aggccgatgg
      901 caaagacggt ggtggcgacc acgacgaaga tcgccccggt cacttcaaaa acgagatgca
      961 gtttccagag cccaatagac gacacaccca acaagccgat ggtggtgaga aaggtcagaa
     1021 atgcgattcc gtagggggat agtcgcggca cggcaagaag cagccagcct gccagcaggg
     1081 ccaacgccag aatggtgccg agaaatggaa cagcaggcga gagcagttgt ccggtaatca
     1141 ggttttgaac gatcagtcca tgcaaaaacg gcgagaattc agggcgcccg aggggcgtaa
     1201 aatagcgttc ttcactcatc gacgtggcaa tgccgacaat gacgatcttc ccggtgaata
     1261 gtgttgggga aacggtctgc atcagaatgt catgtgctga cacgagagga atcatccgtg
     1321 gatcgagatg ataactgatg agtccgtgtt cggagtcgac gagagagaac tgccgggtct
     1381 cggaaccgat caggcaggtc aggccacctg gttcgacccg gaaagacgtt gaatcgaggc
     1441 gcagatggcg ctttgccagt tgcgcctgaa aagagagaac cgcatctccg cgggtgggtt
     1501 gaaaaaagag agagcaggtg cgtactgcct catcaacatg tggtcggaaa taggtgggcg
     1561 cgacgatgcc actgcgtgcc agagcgggaa acggaggggt catctgctgg tcgccgcgtc
     1621 ggtaggcttt cagcacgacg ttgcccagtt gctgcacggt ttcggccagt tccttgtctg
     1681 cggcttcgtc atgagaagcc gtgccgggaa agaagacatc gagcccgatg acacttgggt
     1741 ttccttcgcc gatcaatcgc agcagacggg catacgctcg gcgatcggac tggaacaacc
     1801 ctggagtgcc cagaacggaa cgatcatcga tgacggccag aacgatatcc ttcggttgtg
     1861 ctgctggcgg aagcaaccag gcaataggtg caagccaggg aaactcggtc gggttcgagg
     1921 cgtgaatctg ggtgcgaaac cactgatcgc gcaaacgcgt atccagccac ccgaaaaatg
     1981 gtttgaaaac aagaaggatg agtccgacac tgaatccgaa caggacaatg gcagaccgaa
     2041 aacgggtggt gaatgagggc gtgtcttttg atttcgaacc catggaatct ccggatggta
     2101 cgagcggcgt aatccgctca gggattacgc cgctgttcgt tgctcggtga ggaacgcagc
     2161 atgcagtgag ggctaaggaa aaagtgtcgc gctagaactt gcgaggtccg tcatcacgtc
     2221 ggtcgtcacc atcgcgatca tcaccgccat catccggtcc gtccctgtcg cccggatcga
     2281 atcgcggtgg tcgtgtgcca atgggataag gttcatagcc cggcgttccg gtgtatgccc
     2341 cgtagccatt ttgaatgttc cagccaatct gttttccttc gacgagcaga ttgtcagctt
     2401 cttccaggtc tgccaacggc gct
//
LOCUS       DAORHD010000782         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53010_length_2422_cov_3.553443, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000782 DAORHD010000000
VERSION     DAORHD010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53010_length_2422_cov_3.553443"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKO06_15360"
     CDS             <1..517
                     /locus_tag="PKO06_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV71080.1"
                     /translation="EGFVATIQARLPSKVTVVLTHGHIDHIGGVTQIRRVFKAEVWCG
                     TDDAEMLTDPRKNLSIFFGASFSTEKPDRLLRDGDVIRCGLHEATILHIPGHTPGGVA
                     LAFQQLVLSGDVLFAGSVGRSDFPGGDHELLIDMIKAKLLPLAPRRVFPGHGPATRLE
                     HEARTNPFLNE"
     gene            566..1141
                     /locus_tag="PKO06_15365"
     CDS             566..1141
                     /locus_tag="PKO06_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71081.1"
                     /translation="MEQNFRQQLTDRCVEHGEGAGFQFEVRCDRCHEVWQSEFVPFRQ
                     AQTAGWLHRLTWLFGGGSNVRSMLQDVAGSSTGSGSGLLRLVLGWLRQAGILAHVVDG
                     VAESGFRPARDQALSEAAAKAEKHYHHCPACRHWVCDTCWHPELAHCADCAGNRRLME
                     TVGAAKVCPACRKTLPGAPKFCPDCGHPIEP"
     gene            1454..>2422
                     /locus_tag="PKO06_15370"
     CDS             1454..>2422
                     /locus_tag="PKO06_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNV71082.1"
                     /translation="MAIFEELRRNRHENLFFLQHRESGLRGVLAIHDSSLGPAIGGVC
                     CREVESEDRLAAEALLLAEQASLAAATFGTDCGGGKAILWCRPEQKNEWMFRAFGAYL
                     EGLGGRFIASPDLGTTAADMSHIARETRFVAGVPKPDANEPSDPYTALTARGVFLGIK
                     AAVKAALGASNLSGLKIGVQGLGKVGLALVSLIVAEHGIPYITDRLFEQVKTARDRFH
                     SAVMVRPNDLWETELDVLAPCACSAVLSDSTVERLNCRIVAGAASRQHTDERVIERLH
                     ERGILYIPEFVINGGDMLLIDAELRGQPAAAAVRDVELVYARVEALL"
BASE COUNT          447 a          706 c          779 g          490 t
ORIGIN      
        1 cgagggcttt gtggccacca tccaggcacg actgccgtca aaggtgacgg tggttctgac
       61 gcatgggcat attgatcaca ttggtggcgt gacgcagatt cgccgggtct tcaaggcgga
      121 agtctggtgc ggcacggacg acgccgagat gctgacggac ccacgcaaga atctctcgat
      181 ttttttcgga gcatcgtttt ccaccgaaaa accggatcgg cttctgcgtg acggtgacgt
      241 gatccgctgc gggttgcacg aagcgacgat tcttcacatc cccggtcata cgcctggtgg
      301 agtggctttg gcatttcaac aacttgtact gtcaggcgac gtgctgttcg cgggcagtgt
      361 cggaagatcc gactttcccg gcggtgacca cgagctcctg atcgacatga tcaaggccaa
      421 attgttgccc ctcgctccgc gacgcgtatt ccccggacac ggcccggcga cccgcctcga
      481 gcacgaggcg cgaaccaatc cgtttctcaa cgagtaagcc acgattgaag ccgaggcccg
      541 agacgaacag gagaagacga cgcacatgga acagaatttt cgccagcagc tgaccgatcg
      601 ctgtgtcgaa cacggagaag gtgcggggtt ccagttcgag gttcgctgtg atcgttgcca
      661 tgaggtctgg cagtcggagt ttgttccctt ccgccaggca cagaccgccg gctggttgca
      721 tcggttgacg tggttgttcg gcggtgggtc gaatgtgcga tcgatgctgc aggatgtggc
      781 aggctcctca accggcagcg gaagcggtct gctccggctg gttctgggct ggctccggca
      841 ggcgggaatc ctggctcacg tcgtcgacgg cgttgcggaa tccggttttc gtccggctcg
      901 tgaccaggcg ttgagtgaag cggctgcgaa ggcggaaaaa cactatcacc actgtccagc
      961 ctgccggcat tgggtctgcg atacgtgctg gcacccggaa ctggcgcact gtgccgattg
     1021 tgccggcaat cgccggctca tggagacagt cggagcggcg aaggtctgtc cggcttgtcg
     1081 caagactctc cccggagcac cgaaattctg ccctgactgt ggacatccga tcgaaccctg
     1141 atcgtcagag ccgacctggc gggagctgcg cgggcacaag caggggcctt gagccctcga
     1201 gaagcaagac gagtgtccgg cgcgcgagtt cgcgaagggg ttcgccgaca tccagagcgc
     1261 atggagccgt tcaccggttc ggaggtcccc agactcgcaa gggcggaaaa acttccctca
     1321 tgtggcgggg caccccctca gatccgggag tcctgactct cctgtgtggc gtgaagcggg
     1381 ttctgcattc attgacttgg ccggtgccct ttgctaccat ggctcaactc agggaatcac
     1441 ggaacaggaa tctatggcca ttttcgaaga actgcgtcgc aatcgacatg agaacctgtt
     1501 ttttctgcag catcgcgaaa gcggactgcg cggggttctg gcgattcacg attccagcct
     1561 ggggccggct atcggcggcg tctgctgtcg cgaggtcgaa agtgaagacc ggttagctgc
     1621 cgaagcgctg ctgctggctg aacaagcctc attggctgcg gcaaccttcg gcacggactg
     1681 cggcgggggg aaggcgatcc tctggtgccg ccccgaacag aagaacgaat ggatgtttcg
     1741 cgcctttggt gcttatctcg aagggctggg aggccggttt atcgcctctc ccgatctggg
     1801 caccacggcg gctgatatgt cgcacattgc ccgtgaaacc cgcttcgttg ccggagtgcc
     1861 caaacccgat gccaacgagc cgtcagaccc gtatacggcg ttgacggccc gcggggtttt
     1921 ccttggcatc aaagctgcgg tgaaagccgc cctgggagcc tcgaacctct ccggcctcaa
     1981 gatcggggtt cagggcctgg gaaaggtcgg tctggccctc gtctcgctga tcgtagccga
     2041 acacggcatt ccctatatca ccgaccgtct gttcgagcag gtgaaaacag ctcgcgaccg
     2101 tttccactcg gcggtcatgg tccgtccgaa cgacctgtgg gaaacagagc tcgatgttct
     2161 cgccccttgt gcgtgcagcg cggtactcag cgactcaacg gtcgaacggc tgaattgtcg
     2221 catcgtggcc ggcgcggctt caaggcagca caccgatgaa cgggtgatcg agcggttaca
     2281 cgagcgcggt atcttgtaca ttcctgagtt tgtcatcaat ggtggcgaca tgctgctgat
     2341 cgacgcggag ttgcgcggtc aaccggcggc cgcggccgtg cgtgatgtcg agctcgtcta
     2401 tgccagggtc gaggccctgc tc
//
LOCUS       DAORHD010000783         2421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53071_length_2421_cov_2.319104, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000783 DAORHD010000000
VERSION     DAORHD010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53071_length_2421_cov_2.319104"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /gene="hemB"
                     /locus_tag="PKO06_15375"
     CDS             <1..696
                     /gene="hemB"
                     /locus_tag="PKO06_15375"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNV71083.1"
                     /translation="GVIPLAIRKIRESAKQALIITDVCLCAYTSHGHCGIVNDKGLVL
                     NDESLPYLGEMALAHARAGADIIAPSDMMDGRIGALRALLDLEGFTDTPILSYAAKYA
                     SSFYGPFRDAAHSAPAFGDRRTYQMDPGNRREALCELQSDLEEGADMLMVKPALPCLD
                     VIREARDQFECPIAAYQVSGEYAMIKLAAAQGWLDERQAVNETLTAIRRAGADFIMTY
                     FAIDAANHLVHHS"
     gene            693..1757
                     /locus_tag="PKO06_15380"
     CDS             693..1757
                     /locus_tag="PKO06_15380"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71084.1"
                     /translation="MKRPERPKLLAWETTRACLFTCSHCRASAIPRPMPDELTLTEGR
                     QLLEQAARLGPWIVILSGGEPLLRHDLEDLAQAGIQSGHSVVVACNDGKLLTAERISS
                     LKSAGVSRYSFSMHFAGETDQDRFLGRAGAYAENVQAFARLRETGVQFQINTTVMPGN
                     QQKLPQILSRAKEWGAAAWHLFFVVPTGRAGEHTPEAAATFLSDRETETVLEWVADTA
                     DDAGIPVKETCAPHYARIRAQKRKSVPHGGAGCMAGRGFAFVSSTGDVKPCGYFDQVL
                     GNVRGQPFDKIYTTSPLLQSFRTPERLSGACGACAYRNLCGGCRARAHAQTGDPLASD
                     PACHQAVTCNGNGHEKPVPA"
     gene            1773..2216
                     /locus_tag="PKO06_15385"
     CDS             1773..2216
                     /locus_tag="PKO06_15385"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV71085.1"
                     /translation="MDEKDQEIVRLLQDGFPLDPEPFKVIGDKLWIDEMAVISRLARL
                     HQKGVIRHLGVFLDSSKLGYTGVLAAVQVAAADLDRVCTALAAMDGVTHNYLRDGEPN
                     LWFTLITRTPEERQRLIEQVSQAANGARVMLFPSKRLFKVRTDLD"
     gene            2232..>2421
                     /locus_tag="PKO06_15390"
     CDS             2232..>2421
                     /locus_tag="PKO06_15390"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV71086.1"
                     /translation="MNDQDIRLIRELQNGIKLERRPFLRLAKELNLSEDQVLERVRAL
                     LKEGLIRRVGISVRPHQVG"
BASE COUNT          505 a          740 c          695 g          481 t
ORIGIN      
        1 ggcgtcattc cgctcgccat ccgtaagatt cgcgagtcgg caaaacaagc cttgatcatc
       61 accgacgttt gtctttgcgc ttatacctca cacggtcact gcggcattgt caacgataag
      121 ggactggtac tgaacgacga atctctcccg tatcttggag aaatggccct ggcccacgct
      181 cgcgcaggtg ccgacatcat cgccccctct gacatgatgg atgggcgcat cggcgccctg
      241 cgagcccttc tcgacctcga aggcttcacg gacactccca tcttgtccta tgccgcaaaa
      301 tacgcctctt cgttctacgg cccctttcgc gacgccgccc actcggctcc ggcatttggc
      361 gaccgacgca cctaccagat ggatcccgga aatcgtcgtg aggctctctg cgagctacag
      421 agcgatctcg aggaaggtgc cgacatgctc atggtcaaac cggcacttcc ctgtctcgac
      481 gtgattcgcg aagcccgcga ccagttcgag tgccctatcg cggcctatca ggtatcgggt
      541 gaatatgcca tgatcaaact cgcggccgcc cagggttggc tcgacgaaag gcaggccgtc
      601 aacgagacgc tcacggcgat tcgaagagcc ggtgcggatt tcatcatgac gtatttcgcc
      661 attgatgccg caaaccatct ggtgcaccac tcgtgaaacg ccccgagcgg cccaaactgc
      721 tggcttggga aacgacccga gcctgcctgt ttacctgctc tcactgccga gccagtgcca
      781 ttccacgccc catgccggac gaactgaccc tgacggaagg gcgacagctg ctcgaacagg
      841 cggcacgcct cgggccctgg atcgtcattt tgtctggcgg cgaacccttg ttgcgtcacg
      901 acctggaaga ccttgctcag gcgggcattc aatcgggaca ctcggttgtc gtcgcttgta
      961 acgacggcaa gcttctgact gcagaacgaa tctccagcct gaagtcggca ggggtcagcc
     1021 ggtacagctt cagcatgcac tttgcgggag aaactgatca agaccggttt ctcggccgag
     1081 cgggagctta tgcggaaaac gtgcaggctt ttgcccgtct gcgggaaacg ggcgtccagt
     1141 ttcaaatcaa caccaccgtc atgccgggca accagcagaa actcccgcag attctctcgc
     1201 gcgccaaaga atggggtgca gcggcatggc atctgttttt cgtcgttccg accgggagag
     1261 ctggtgagca cacgcctgaa gccgcggcaa ccttcctgtc cgaccgcgaa acagaaaccg
     1321 ttctcgagtg ggtggcggat acggcggacg acgccggaat tcccgtcaag gagacctgtg
     1381 cgccccacta cgcacgaatc cgggcgcaga aacgcaagtc cgtgccgcac ggtggagccg
     1441 ggtgcatggc cggtcggggt ttcgctttcg tcagcagtac gggcgacgtg aaaccctgtg
     1501 gatacttcga tcaggtgctc ggaaacgtgc gaggacaacc gttcgacaag atctacacca
     1561 cctcgcccct gttacagtcg tttcgtacac ccgaacggtt gtccggagcc tgtggtgcgt
     1621 gcgcctatcg aaatctgtgt ggcggttgcc gcgcgcgcgc ccacgcgcaa accggtgatc
     1681 ccctggcttc tgatccggcc tgtcatcagg ccgtgacctg caacggaaac gggcatgaaa
     1741 agcctgttcc agcatgagaa aggaatgaag gtatggacga aaaagatcag gaaattgtac
     1801 gcctgcttca agatggcttt ccccttgatc ccgaaccatt caaggtcatc ggcgataagc
     1861 tctggatcga cgagatggcg gtcatttcac gactcgcccg attgcaccag aaaggggtga
     1921 tccggcatct gggggtgttt ctggacagca gcaaactcgg ttataccgga gttcttgccg
     1981 cggttcaggt cgccgccgcc gatctcgatc gcgtgtgtac cgctctggcc gcgatggacg
     2041 gagtcacgca caactatctg cgtgatggcg aacccaatct ctggttcaca ctgatcacgc
     2101 ggacgcccga ggaacgccag cggctgatcg aacaggtcag tcaagccgcc aacggcgccc
     2161 gggtcatgct ctttccctcg aaacggcttt ttaaagtgcg cacggacctc gactgaaaca
     2221 ggagttacac catgaacgac caggacattc gcctgattcg tgaactgcag aacggcatca
     2281 aactggagcg acgtccgttc ctgcgactgg ccaaggaact caatctctct gaagatcagg
     2341 ttctggagcg agttcgcgcg ttactgaaag agggattgat tcggcgcgtc ggtatttcgg
     2401 tgcgccccca tcaggtcggc t
//
LOCUS       DAORHD010000784         2420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53099_length_2420_cov_3.127696, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000784 DAORHD010000000
VERSION     DAORHD010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53099_length_2420_cov_3.127696"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKO06_15395"
     CDS             <1..391
                     /locus_tag="PKO06_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71087.1"
                     /translation="WGVFLKKSCTIDKVEDFYQITMTMTRKALANTAARLYVVFNTAK
                     PTTSRAVQRWVGGLRKAESHGRRIPADFLDQSAQFLAENVEAERLRESGASYSKALSR
                     CLDALERFEAVHTIPHLTPESLLQITN"
     gene            417..489
                     /locus_tag="PKO06_15400"
     tRNA            417..489
                     /locus_tag="PKO06_15400"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Asn,seq:gtt)
     gene            complement(600..1337)
                     /gene="trmB"
                     /locus_tag="PKO06_15405"
     CDS             complement(600..1337)
                     /gene="trmB"
                     /locus_tag="PKO06_15405"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNV71088.1"
                     /translation="MRVGKLDRYRQLKELENVVEPSCHELLAAPSQLSGKWSGDFFKR
                     PGELVLEIGCGWGDFALYLARQRQGCNVLGLDIKGARLWRGARQALHEKISNVGFIRV
                     AAEFVDRLFATGELSEIWVTFPTPWLWKPEKMLISPPFLARYRQTLRMNGVLHLKTDV
                     ESLADYACHIWPMVGFQILERQQHVTVTARGDAEGLSRFETRAVQSQKPIWHIRAIST
                     SEAVQSVPDDLVRHPWNSIYRTRSGKP"
     gene            1464..1952
                     /locus_tag="PKO06_15410"
     CDS             1464..1952
                     /locus_tag="PKO06_15410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV71089.1"
                     /translation="MNIEIVQVTTNEQWHAALQIREDVFVREQQVPLELERDAIDEQA
                     QHILALVTNLSQTPATGNNPGANGAPVWAGTARAFRNPECGEELMIGRVAVRKPFRRL
                     GIAGKMTNWLIDWARSGGYQRVRLHAQSYIVRLYARLGFVTHGAEFLEAGIPHQEMIL
                     KL"
     gene            complement(1991..>2420)
                     /locus_tag="PKO06_15415"
     CDS             complement(1991..>2420)
                     /locus_tag="PKO06_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71090.1"
                     /translation="LRVRRDQLSQLATKAALLHHQGDLEKALEELRSQEQACRKLGYR
                     KELAQCLGSQADILRERGQAARAIELLSQTEEIWRSTGNQAGLAEMLIARAVVNILDF
                     NKPTEAAADLAEATRIAATHQISDASKELLNHLNEFMKQP"
BASE COUNT          540 a          655 c          689 g          536 t
ORIGIN      
        1 ctggggagtc tttttgaaga agtcctgcac cattgacaag gtcgaagact tttatcagat
       61 caccatgacc atgacgcgaa aagcccttgc caatacggca gcgcgcctgt atgtggtatt
      121 caataccgca aagccgacca cctcgcgagc ggtgcaacgc tgggtcggtg ggctgcgaaa
      181 ggcagaatct cacggacggc gtatcccggc tgattttctt gatcagtccg cgcagtttct
      241 tgctgaaaac gttgaggccg agcggttgcg tgaatccggc gcgtcgtact caaaagctct
      301 gtcacgctgt ctcgatgccc ttgaacgttt cgaagcggtt cacacgattc cgcatttgac
      361 gccagaaagt ctcttgcaaa tcaccaattg atgtggtata aatcaactgt tcacattcct
      421 ggttagctca gtcggtagag cagctggctg ttaaccagcg ggccgggggt tcaaatccct
      481 caccaggagc tctctcagat ttttgtatga acagaaaacc cgcctctcag gcgggttttc
      541 tgcatatggc aacttgcaat gtgcgctgac catgcgcacg gaatttcggg tgacaacgat
      601 cagggttttc ccgaacgtgt tctgtagatg gaattccagg ggtggcggac gagatcgtcg
      661 ggcacagatt gtacggcttc agatgtcgag atggcgcgga tatgccagat gggtttctgg
      721 gactgaacgg ccctggtttc gaatctcgac aggccttctg cgtcgccccg cgcggtgacc
      781 gtcacatgct gttgtcgctc cagaatctga aatccgacca tgggccagat gtggcaggca
      841 taatcagcca gtgactcgac atctgttttc aggtgcagca caccgttcat acgcaaggtc
      901 tggcgatacc tggccagaaa cgggggggag atcagcattt tttccggttt ccacagccag
      961 ggtgtcggaa acgtcaccca gatctcgctc agctctccgg tggcgaacag gcgatcgacg
     1021 aactccgcgg ccacacggat aaagccgacg ttcgagatct tttcgtgaag agcttgccga
     1081 gctcctcgcc agaggcgagc tcccttgatg tccagaccca ggacgttaca gccctgacgc
     1141 tggcgggcga gatacagcgc aaagtcgccc cagccgcagc cgatttcgag aaccagttct
     1201 cccgggcgct tgaagaaatc gccggaccat ttccctgaca gttgagatgg agctgcgaga
     1261 agttcgtggc aggaaggttc gaccacattc tcaagttcct tcagttgtcg atagcgatcg
     1321 agtttgccga cacgcatctg aaaatggttc cattccgttg cgacgatacc cgagattcac
     1381 gaaagcgatg ccctcgcatc aattctgaac cagaggtatc attataccag cgacgcttca
     1441 gaaaaacagg atcagacttg atcatgaaca ttgaaatcgt acaagtaaca acgaacgaac
     1501 aatggcatgc tgcgctgcag atacgagaag atgtatttgt tcgcgaacag caagtgccgc
     1561 tcgagcttga gcgcgatgcc atcgatgagc aggctcagca cattctcgcc ctcgtgacca
     1621 atctctcgca gacgccagcg acaggcaaca acccgggggc gaacggggcg cccgtctggg
     1681 ctggaacggc acgggcattt cggaacccgg aatgcgggga agaactgatg atcggacgag
     1741 ttgccgttcg aaagccgttt cgacggctgg gcatcgctgg aaagatgacg aactggctga
     1801 tcgactgggc tcgctctggc ggttaccagc gggttcgctt gcatgcgcaa tcgtacatcg
     1861 tcagattgta tgcgcgtctc ggtttcgtta cgcacggggc agagtttctc gaagccggaa
     1921 taccacatca ggaaatgatt ctgaagttgt gaaccggtac aaacgaaagg gacaacgtgt
     1981 ccgaccgccc ctacggttgt ttcataaact cattcaagtg attgagcagc tcttttgaag
     2041 cgtcggagat ctggtgggtc gccgcgatgc gggtcgcttc agctagatca gccgctgctt
     2101 ccgtgggctt gttgaaatcg agaatgttca ccacggctcg cgcgatcagc atttcggcaa
     2161 gtccggcctg attgccggtt gagcgccaga tttcctctgt ctgggaaaga agctcgatcg
     2221 ctcttgctgc ttgcccacgc tcacgcagaa tgtcagcctg actgccgaga cactgagcca
     2281 gttccttgcg atacccgagt ttccggcagg cctgttcctg cgacctgagt tcctcgagcg
     2341 ctttctcgag atcgccctga tggtgcagta acgcggcttt ggttgcaagc tgagacagct
     2401 ggtcgcgacg gacacgtaag
//
LOCUS       DAORHD010000785         2418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53156_length_2418_cov_4.353788, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000785 DAORHD010000000
VERSION     DAORHD010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53156_length_2418_cov_4.353788"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PKO06_15420"
     CDS             <1..547
                     /locus_tag="PKO06_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV71091.1"
                     /translation="LLSLPGSPVLYYGDELGMGDNVYLGDRNGVRTPMQWSMDRNAGF
                     SRADSARLYSPVIVDPVYDYRHLNVESQLRVPTSLLNWVKRLIKIRKRYQAFGRGTIR
                     FLKNSSSRVITFLREWEDQKLLVAANLSRFAQPCEIDLSEFDGWKPIEVYANLAFPAI
                     GRLPYFLTLGPHSFYWFELRG"
     gene            complement(544..>2418)
                     /locus_tag="PKO06_15425"
     CDS             complement(544..>2418)
                     /locus_tag="PKO06_15425"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71092.1"
                     /translation="AGSLLFAHLLCFGFVLPLRLRGKLLIGALFAIGVPLGILLPNAI
                     LQQRLQAERLRETIINRLSVQQHLLAHQFQAYMSELRLTLLQRAEQLRPVACSSFQLA
                     SVLADTFSGLPVQDVFGVSADRQQFHWVPPLTPTRDTSNMERTALLAMAQAFRLGSDP
                     ATRHQKPLVKVHGVYMNLTPVGDLLSRPGTIITSDMLAGKTMTVTGLPIPVATGAAAI
                     LVRFRPADLLSHFFATHHTLLQAAQENVDEWHLSQVALQLSSDQSRQVVGCFWEPRPD
                     FSRPLIPSLDRPLRLGLNIDHQRLETATSTRLLTSIAIPNQPCLLVGCAETTVARSRY
                     VLIWSAVLYLALLLTFIVLLLETVFRIPILFFHAAAGEIESGRYPSIPPVSGDDELGQ
                     LGDAFGGMIQGLRQRDAMSQFVSDEVLEAVQSDTGEGLAPGGELMPVTVLFVGLRGFK
                     DRALSLPAADRLRIMNSFLDIAGMELKAHQGSLDKMIEDTIMGVFRPRGSPTPTSVRA
                     VKAGLGLQKKLSEFWNSSGIACDIGIAHGTVLSGRIGSHHGTLDFSVIGNAVNLAARL
                     KAAAKGAMTSGILIDPQAIRDTRGAVRLNFLKRTSIKGRSREFNLYEVTGLRDESPES
                     "
BASE COUNT          517 a          651 c          772 g          478 t
ORIGIN      
        1 gctgttgtcg cttccgggct cgccagtgct gtactacggc gatgagttgg gcatgggaga
       61 caatgtctac cttggcgatc gcaacggcgt gcgaacaccg atgcagtgga gcatggatcg
      121 caacgccggg ttttcgcggg ctgattcggc acgcttgtac agccccgtga tcgtcgaccc
      181 ggtctatgat tatcggcatc tcaacgtcga gtcccagtta cgtgtgccaa catctctgct
      241 caactgggtg aaacgactga tcaaaattcg caagcggtat caggcgtttg gtcgcggcac
      301 gattcgcttt ctgaagaact cgagttccag ggtgatcacc tttctgcgcg aatgggaaga
      361 ccagaagctg ctggtggcgg caaacctctc gcgtttcgcg cagccctgcg agatcgatct
      421 gtcggagttt gacggctgga aaccgatcga agtctacgcc aatctggctt ttccggccat
      481 cggcaggctt ccctatttcc tgacgctggg accgcacagt ttctactggt tcgagctgcg
      541 cggctaggac tctggtgact cgtcgcgcag gccggtgact tcgtagagat tgaattcgcg
      601 cgatcgaccc ttgatgctgg tgcgtttgag gaagttgaga cgtaccgctc cgcgcgtgtc
      661 gcggatggcc tggggatcga taagtatgcc ggaggtcatc gcccctttcg cggctgcttt
      721 gagtcgggcg gcgaggttga ccgcatttcc gatcacggaa aaatcaagcg tgccgtggtg
      781 cgacccgatg cgccctgaca ggacggttcc gtgggcgatt ccgatgtcgc aggcgatgcc
      841 agaagaattc cagaactcgg aaagtttctt ctgaagccca agacctgctt tgacggctcg
      901 gacggatgtc ggggttggac tcccgcgggg gcggaacacg cccatgatgg tatcttcaat
      961 cattttgtcc aggctgcctt gatgggcttt cagctccatc ccagcgatat cgagaaaaga
     1021 gttcatgatg cgcagccggt cggcggccgg caacgacaac gcacgatcct tgaagccgcg
     1081 caggccgacg aagagaaccg tcacgggcat caactctccg ccgggtgcca gtccttcgcc
     1141 tgtatcgctt tgcacggctt ccaggacttc gtccgagacg aactgcgaca tggcatcgcg
     1201 ctgcctgagc ccctggatca tgccgccgaa agcgtcgccc aattgtccga gttcatcgtc
     1261 accagaaaca ggaggtatcg aaggatatcg acctgattca atttcacccg cggcagcatg
     1321 gaagaacaga atcggaattc ggaacacggt ttcgagcagc aagacgatga aagtgagcag
     1381 cagcgcgagg tagagcacgg ccgaccagat gaggacatac ctggatcggg caaccgtggt
     1441 ttccgcgcag ccgaccagca gacagggctg gttgggaatg gcgatcgacg tcagcagacg
     1501 ggtcgaggtg gcggtctcga gccgctgatg atcaatgttc agaccaagtc ggagtggcct
     1561 gtccagagag ggaatcagcg gacggctgaa gtcagggcgt ggttcccaga agcagccgac
     1621 gacctgtcgc gactgatcag atgagagttg tagcgcgact tgagacagat gccattcatc
     1681 cacgttctcc tgagcggcct gcagcagagt gtgatgggtg gcaaaaaagt gcgagagaag
     1741 atctgcggga cggaaacgaa ccaggatcgc agccgcgccc gttgccacag gaatgggcag
     1801 gccggtgacg gtcatcgtct ttcccgccag catgtccgag gtgataatgg tgccaggcct
     1861 cgataacagg tctcccacgg gagtcaggtt catgtacaca ccatgcactt tgacgagagg
     1921 tttctgatgg cgggttgccg gatcggatcc gagccgaaaa gcttgggcca tggccagcag
     1981 agctgtgcgt tccatgttgc tggtatcccg ggttggcgtg aggggcggaa cccagtgaaa
     2041 ttgttgtcgg tcggcggaga caccaaagac atcctgcact ggcaggcccg aaaacgtgtc
     2101 tgcaaggact gatgccagtt ggaaactgga acaggcaacg ggacgaagtt gttcagctcg
     2161 ttgcagcagg gtcagacgca gttctgacat gtacgcctga aactggtgcg ccagcagatg
     2221 ctgctggacg ctgaggcgat tgatgatggt ttcgcgcaga cgctcggcct gtagccgttg
     2281 ctgaagaatc gcattcggga gcaggatgcc cagggggact ccaatggcga acaacgctcc
     2341 gatgagcagt tttccacgca gccgaagcgg gaggacgaag ccgaaacaca gcaggtgcgc
     2401 gaacaacagg ctgccggc
//
LOCUS       DAORHD010000786         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53204_length_2417_cov_3.845893, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000786 DAORHD010000000
VERSION     DAORHD010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53204_length_2417_cov_3.845893"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PKO06_15430"
     CDS             <1..280
                     /locus_tag="PKO06_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015223961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNV71093.1"
                     /translation="PIDPKTSNIEAKDIAGQTKQVMANLEAVLHAAGTDFSKVVKSTI
                     FLVDLGHFATVNEIYAKAFPKEPPARSCIQVAKLPKDSLVEIEMIALV"
     gene            complement(673..>2417)
                     /locus_tag="PKO06_15435"
     CDS             complement(673..>2417)
                     /locus_tag="PKO06_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV71094.1"
                     /translation="WKGDRFMKKVLSSMPAQAALLLVLSCFALATLLPGCGANPSGPS
                     TSVSTPQTPTAASGSAVVYGSVTDLETGSQVSGSGFTVSLYLDSTLVSSRSLNNGGFT
                     FNGLSQGIYTLFVTDSTNTYSSNFQLVNVSTSGQFQTEIKLRRIATAVGIRLVNLWGK
                     ITSSDGSGIPFARVTLGTSAFETTTMIDGSYYLFNVPQSDVPYDLKVTKSGYNDKTQS
                     LIIGAATITLNGAEKNSQITKTDQTATSRTGYDLGTAVLTGKPTTTAAITGYVKTPKG
                     DVVPNFTFKLFHRKDLSDTSQITEQRTVISNAAGYFSETTLPKGYYFAAATNAKPKKE
                     SDGTTFIWSIDGTVYASNIEAISDTAAEAIITIANNTPGINTPALTAPANLVRYTSDQ
                     NADFSWTGVGTATSYVVRLLNPPANPTGRVYNLELPAPSTGALSQNISFFGLPIGYYN
                     WQVGAVDVSTNQTIYSNPRSFLVAPDRNSLSPAYDQVIMPGGAASPSITLRYPSDPNA
                     SKIEVEIFDAAGNRLADPKDTEFGTDSVTYTWDGAWTNAIPEMWQWQVTYWYAGSTIK
                     MTSERTRFYRVRNP"
BASE COUNT          550 a          621 c          649 g          597 t
ORIGIN      
        1 ccccattgat ccgaagacgt cgaacatcga agcgaaggat atcgccggcc agacgaaaca
       61 ggtcatggcg aatctcgaag ccgtactgca cgcggccggc accgatttct cgaaagtggt
      121 gaagagcacc atctttctcg ttgacctcgg tcatttcgcg acggtgaacg aaatctacgc
      181 caaagccttc cccaaggaac cacccgctcg ctcgtgcatt caggtggcca aactgccgaa
      241 agactctctc gtcgaaatcg agatgatcgc cctggtttga cctgacctga ttctcacgcc
      301 cgcgacctct ccccgccgta atcctggcgg ttatctgccc tgcggctcgt tttcacctct
      361 tccggcttga agatggcaga gagtttatgc aaatctcaag ttgatgaaat ttgcatagcc
      421 agaacagcgg gggtcgcggg aaggcgagtg gcgaggctgt gcggtcagcg cagcccgagc
      481 tgggttttga tcgcctggac aatcttgttg ggtaagactc aaccaccaga cttcaccgcg
      541 cctcatgatc ggttccttca atatgcgtct tgtctacgtg taatcgcgcc gttccgcaag
      601 gtacgctgag ctggaacgca aagtcgccag aaagaaaaac gcccgacctt ccggtcgggc
      661 gttcgctttg tatcagggat ttcgcacccg atagaacctg gttctctctg aggtcatctt
      721 gatggttgat cccgcatacc agtaggtcac ctgccattgc cacatttccg gtatcgcgtt
      781 ggtccatgcc ccgtcccaag tgtaggttac gctatcggtt ccaaattccg tatccttcgg
      841 gtctgcaagg cggtttccgg cagcgtcaaa aatctctact tcgattttgc tggcgttcgg
      901 atcagacggg tatcttaacg tgatcgaagg agatgccgca ccacctggca taataacctg
      961 atcataagcc gggctgaggc tgttgcgatc tggtgctacc aagaaactgc gagggttgga
     1021 gtagatcgtt tggttcgttg acacatcaac cgcaccgacc tgccaattat agtaaccaat
     1081 tggcaaaccg aagaagctga tattctgtga aagcgcgcct gtcgaggggg caggaagctc
     1141 gagattgtag acccgtcccg tcgggttggc aggcggattc aacaagcgaa cgacatatga
     1201 cgttgctgtt ccaacgccag tccagctgaa gtctgcattc tgatcggaag tgtagcgcac
     1261 aagattggct ggtgcagtca gagctggagt attgattcct ggtgtgttgt ttgcgatggt
     1321 tatgattgct tccgcagctg tatcagaaat tgcttcgata ttcgaggcgt acacagtgcc
     1381 gtcaatgctc cagataaagg tcgtgccgtc tgattctttc ttcggcttgg cgttcgtggc
     1441 tgccgcgaaa tagtaaccct tgggtaaggt ggtttcagag aaatacccag cagcattgga
     1501 gatgaccgtt cgctgctctg taatctggct cgtatccgat aggtctttcc gatggaacag
     1561 cttgaatgtg aagttaggaa caacatcgcc ctttggcgtt ttaacgtaac cggtgatcgc
     1621 cgcggttgtt gtcggttttc ccgtcagaac cgcagtccca agatcatatc cggtacgtga
     1681 ggtggcggtc tgatcagtct tggttatctg tgagttcttt tcagctccgt tcagagtaat
     1741 ggttgccgcc ccgataatga gactctgtgt cttgtcattg taaccgctct tcgtcacttt
     1801 cagatcgtaa ggaacgtcgc tttgaggaac attaaaaagg tagtagctac cgtcaatcat
     1861 ggttgtggtc tcaaacgcgc ttgttcctaa cgtaacgcga gcaaatggga ttccacttcc
     1921 atcggaggag gtaatcttcc cccacaagtt gacgaggcga atgcctacag ccgtcgcaat
     1981 tcgtcgaagt ttgatttccg tttgaaactg acccgaagtg cttacgttga ccaactgaaa
     2041 gttgcttgaa tacgtgttgg tggagtcagt aacaaacagt gtgtaaattc cctggcttaa
     2101 accgttaaaa gtgaacccgc cgttattcag gctcctcgaa gagaccaagg tgctatccag
     2161 atacagagat acagtgaatc ccgacccaga tacctgggat ccggtttcaa gatcagtgac
     2221 tgatccgtaa acaacagcac tgccactcgc tgccgttgga gtttgtggtg tcgaaacgct
     2281 cgttgacggg ccggacgggt tggcaccgca gccgggcaag agggttgcca aagcgaaaca
     2341 gctgagaacc agcaagagag ccgcttgcgc gggcatcgag gagagaactt ttttcataaa
     2401 cctgtctcct ttccagg
//
LOCUS       DAORHD010000787         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53263_length_2415_cov_3.612712, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000787 DAORHD010000000
VERSION     DAORHD010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53263_length_2415_cov_3.612712"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PKO06_15440"
     CDS             <1..1212
                     /locus_tag="PKO06_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71095.1"
                     /translation="PQARKALDVWQKRHPLNSTICFLESLYNAKTADAPGAIAWARRA
                     VEMNPDRTTQLRPLVIYTELQNDPVVGPRIAVLRQAPKSNAPVPLPPPTAGLIPRAHG
                     STPPAAAAAPATPALPAPTAASTAPTPAIPDAPLVVTGADSGERYALETNAQPPHIVE
                     GLKQGIDAALDRVETLFGVKPRTIRIALTPSGTSQSNTAQFAFDTGKISLAVHYLDFP
                     AIKTALDTERPDLDENQKTELSQNLGGHTLSGEIAQALLFQLVKNLELKMLDTGWIRL
                     GLAEVAGGNEVVIKDRLVYTQTRIASGELKLQPIDQVNQGLNQGTGDFTVFQASRIQS
                     YLMVSFLLKKANSVNEGFTKLVAVLKDLVAGKTLAQSLPTRFKISISDFEAGWKEAAF
                     WALKNGQPYEW"
     gene            complement(1254..>2415)
                     /locus_tag="PKO06_15445"
     CDS             complement(1254..>2415)
                     /locus_tag="PKO06_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71096.1"
                     /translation="HKNGDLIDVGNGFRMSPLVYSYNHPYMPAVSSHEIVASQTSIEL
                     WRQLLPLAQRSLQENVERVEPLMNERGEREFVKTRFFASDFLVGLGVIREEVSQRLQS
                     LWWGARLLLLLLICFWGVQREVGRYLSRPLEILLTALQAAAAGNLHWRLELPTRDEFG
                     ETAAVFNHMAEKLIEREKMSRFVSDDILQRVAIGEESAIQPGQGEQVETTVLVSDIRS
                     FTTLSEQYPPDQMVEMLNDYFTAMEDGIASEQGTIVSYIGDAIVAHFPLRSGLDDCAQ
                     RAVRAAWKMRLALTELNRQRQSTGRFAVRTGIGLATGILVSGVAGSTQGRLTQILLGE
                     PFERATALEVNSKRSRFSGIMVDRDTALRCQKDFEMSPLPDEPETIEIRSQK"
BASE COUNT          513 a          797 c          647 g          458 t
ORIGIN      
        1 cctcaggcac gaaaggctct tgatgtctgg cagaagcgac acccgctcaa cagcaccatc
       61 tgctttctcg aatccttgta caatgccaaa accgctgatg cacctggcgc gatcgcctgg
      121 gcaagacgag ccgtcgaaat gaaccccgac cgcaccaccc aactgcggcc actcgtcatc
      181 tataccgagt tgcagaacga tccggtcgtc ggaccgcgga tcgccgtgct ccgacaggct
      241 cccaagtcca acgctcctgt ccccctgccg ccaccaactg ccggtctgat cccgcgcgcc
      301 catggttcca ctccccccgc ggctgcggcc gcgcccgcga ctcccgcgct acccgctccc
      361 acggcggctt caacggcacc cacgccggcg attccagacg caccgctggt cgtgaccgga
      421 gccgacagcg gcgaacgcta cgcactcgaa accaacgcgc agccgccgca catcgtcgaa
      481 ggcctgaaac agggcatcga tgcggctctc gatcgtgtcg aaaccttgtt cggtgtcaag
      541 ccgcgcacca tcaggatcgc gctgacgccg agcggaacct cgcagtcgaa taccgcgcag
      601 tttgcgttcg acaccggaaa aatcagcctc gccgttcatt acctcgattt tccggcgatc
      661 aagacggccc tcgacaccga gcgcccggat ctcgacgaga accagaaaac cgagttgtca
      721 cagaacctcg gcgggcacac cctttccggc gagatcgctc aggccctgct gtttcaactg
      781 gtgaagaacc tcgaactgaa gatgctcgac accggctgga tcagactcgg actcgccgaa
      841 gtggccggtg ggaacgaagt ggtcatcaag gaccgcctgg tttacacgca gacccgcata
      901 gccagcggcg aactcaagct gcagccgatc gatcaggtca atcaggggct caaccaggga
      961 acgggcgact tcaccgtgtt tcaagcctct cgcattcagt cctacctgat ggtgtccttc
     1021 cttctgaaga aagccaactc ggtcaacgag ggttttacca agctcgtcgc ggttctcaag
     1081 gacctcgtcg ccggaaaaac cttggctcag agcctgccta ctcgcttcaa gatcagtatc
     1141 agcgactttg aagccggctg gaaggaagcc gctttctggg ctctcaagaa tgggcaaccg
     1201 tacgagtggt gatccggacc gatctcacgg ccgcgtcacg gatcgcggtg cgtctatttt
     1261 tgtgacctga tctcgatcgt ttccggttca tccggaaggg gactcatctc gaagtccttc
     1321 tgacagcgga gagcggtatc gcgatcaacc atgatcccgg agaaccggga tctcttgctg
     1381 ttgacttcga gagcggttgc ccgttcaaac ggctctccca gcagaatctg cgtcagtcgt
     1441 ccctgcgtcg aacccgccac accagatacc aggatacccg ttgccagtcc aatgccggtt
     1501 cttaccgcga agcgaccggt actctggcgc tggcgattca gttcggtcag cgccagacgc
     1561 attttccagg cagcccgcac cgctcgctgt gcacaatcgt ccaggccgct tcgcaaggga
     1621 aaatgcgcca caattgcatc tccaatgtag gaaacgatgg ttccctgctc ggaagcaatc
     1681 ccgtcctcca tcgccgtaaa gtagtcgttg agcatctcga ccatctgatc cggcggatac
     1741 tgttcggaaa gggtcgtgaa gctgcggatg tctgagacca gaacggtcgt ctccacttgc
     1801 tctccctggc ccggttgaat cgccgattct tcgccgatcg ccacgcgctg tagaatatcg
     1861 tccgagacaa agcgcgacat cttctcgcgt tcaatcagct tttcggccat atgattgaac
     1921 accgcggcgg tttctccgaa ctcatcgcgg gtcggcagct ccagcctcca atgcagattt
     1981 ccggccgccg cggcctgcag agcggtcaac agaatctcga gcggtcgcga gagataccgc
     2041 ccgacctcgc gctgcacccc ccagaagcag atcaacagca ggagaagaag tcttgctccc
     2101 caccagagag actggaggcg ctgcgagact tcttcacgga tcaccccaag tccgaccagg
     2161 aaatcactgg cgaagaaacg tgttttgaca aactcccgct cgcccctctc attcatcagt
     2221 ggctccaccc gctcgacatt ctcctgcagc gaacgctggg ccagcggcag aagttgtcgc
     2281 cacagctcga tagaggtctg tgaggccacg atctcgtgcg acgaaacggc gggcatgtag
     2341 ggatgattgt agctgtagac caggggtgac atccgaaatc cattgcccac atcgatcagg
     2401 tcaccatttt tgtgc
//
LOCUS       DAORHD010000788         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53439_length_2410_cov_4.197028, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000788 DAORHD010000000
VERSION     DAORHD010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53439_length_2410_cov_4.197028"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..751
                     /locus_tag="PKO06_15450"
     CDS             80..751
                     /locus_tag="PKO06_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV71097.1"
                     /translation="MSRILIIEDEPVIRELLRDNLDFEGFEVVEAADLAAGRRAQLTN
                     PDLVLLDVNLPDGDGIRQLQEWRTAGVTIPVVVCTVRDREIDVVRALEAGADDYVTKP
                     FRIRELIARIKAVLRRHQPVATPVTRIGPNEINFSAREARRHGEPLTLTATEWTLLEY
                     LSRNAGQVVSREQIMNHVWGVDELEDSRAVDVHISRLRRKLEPAGSAPLLHTVRGLGY
                     RLDRA"
     gene            855..2309
                     /locus_tag="PKO06_15455"
     CDS             855..2309
                     /locus_tag="PKO06_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71098.1"
                     /translation="MYIRTIALIFALSAVCLVPGFGQTPDSGRESQLQQTLVQMWVDG
                     PTAAHEKQLKELASGSDSIAARALVQLGCLARMKGDATLEAETLKKLEGFKEQKGELE
                     GLSRLLAPAGQKRVMKVDLRDVLIPEAMRIIARSADRAVIVHGEIPEKKISLFLPEVE
                     FDKVMQILADMGSCKIEKLDNLLVVSPKDFAASTTADTADNLISLDLKDVSIRDAMRL
                     VAKRADINLVLHRSIGGVVTVQLKAVKPLDALRMLAKANDLHLEEDGSYLFVVPREKM
                     YDVIGRSDMRTVKLNHLPAEKALEIVQQQTTCNGEVAGDRRLHLRGNPDVLDRAAKIL
                     SELDKPAKPFTTDFRIWESVDGTRLKADEFLALPEEKKLAVARIIARPRIITLPGSPA
                     KISINSDSDDTDKNARFHKINLTADLLPLPDGMVRFKTGFFIELKSKDAKGIKETKRQ
                     GESTYVVKPGATFGQDLETENGTVLIEYTVRQAE"
BASE COUNT          522 a          764 c          709 g          415 t
ORIGIN      
        1 agggttcgtt cgaactgcgc gcccgggacg gcggcggcac cgaagccctc acgacctggc
       61 cgaccaagga gtagatcgta tgtctcgaat tctcatcatt gaagatgagc cggttatcag
      121 agaactgctg cgagacaacc tggactttga agggttcgag gtcgtcgaag ccgctgacct
      181 ggccgcgggt cgtcgggcgc aactcaccaa ccccgacctc gtgctgctcg acgtgaacct
      241 ccccgatggc gacggcattc gccagctgca ggaatggcgc acggccggag tcacgattcc
      301 cgtcgtggtc tgcaccgtcc gcgatcgcga gatcgacgtg gtgcgtgccc tcgaagccgg
      361 tgccgacgac tatgtcacga aaccgtttcg gatccgcgag ctgatcgccc gcatcaaagc
      421 cgtgctgcgc cgccaccagc ccgtcgccac gccggtgacc cgcatcggtc cgaacgagat
      481 caacttttcg gcccgcgaag cgcgccgcca cggcgaaccg ctgacgctga cggcgaccga
      541 gtggaccctg ctcgagtatc tgtcgcgcaa tgccggccag gtggtcagcc gcgagcagat
      601 catgaaccac gtctggggcg ttgacgagct cgaagactcg cgcgccgtcg acgtgcacat
      661 cagccgcttg cgccggaaac tcgaaccagc cgggtcggcc ccgctgctgc acaccgtgcg
      721 cggtctcggc taccgactcg acagagcgtg agaatcgatt gttacacaac tgttacacag
      781 ccgtcatgga atcgtgaccc tcgcgagcga tactgaaact gtccgaagaa aaaaacatcg
      841 aaggagcacc tcgcatgtat atcagaacta ttgccctgat ctttgctctc agtgccgtct
      901 gtctcgtgcc cgggttcgga caaacccccg atagcgggcg cgagtcacaa ctgcagcaga
      961 ccctggtaca gatgtgggtc gacggcccga ccgccgctca cgagaagcag ctgaaagaac
     1021 tggccagtgg tagcgactcg atcgccgccc gcgccctggt acagctcggc tgtctcgccc
     1081 gcatgaaggg tgatgcgact ctggaagcag aaaccttgaa aaaactcgaa gggttcaagg
     1141 agcagaaagg cgaactcgag gggctgtcgc gtctgctcgc cccagccgga cagaagcggg
     1201 tcatgaaagt cgatctgcgt gatgtgctga taccagaggc gatgcgcatc atcgcccgtt
     1261 cggccgaccg cgccgtcatc gtgcatggcg agattccgga aaaaaagatc tcgttgttcc
     1321 tgcccgaagt cgagttcgac aaggtgatgc agattctggc cgacatgggc tcgtgtaaaa
     1381 tcgagaagct cgacaacctg ctggtggtca gcccgaagga cttcgcggcg agcaccaccg
     1441 ccgacacggc tgacaacctg atttcgctcg acctcaaaga tgtctcgatt cgcgatgcga
     1501 tgcgcctggt ggccaaacgg gccgacatca acctcgtgct gcatcggtcg atcggcggcg
     1561 tggtcacggt gcagctgaag gcggtgaagc cactcgacgc gctgcgcatg ctggccaagg
     1621 ccaacgacct gcatctcgag gaggacggca gctatctgtt cgtcgtgccg cgcgagaaga
     1681 tgtacgatgt catcggcaga tcggacatgc gcacggtcaa gctcaaccac ctgccggcgg
     1741 aaaaggcgct cgagatcgtg cagcagcaga ccacctgcaa cggtgaagtc gcgggtgacc
     1801 gcaggctgca cctgcgcggc aaccccgacg tgctcgatcg cgccgcgaag attctgtccg
     1861 agctcgacaa acccgccaag cccttcacca ccgattttcg catctgggaa agcgtcgatg
     1921 gcacgcgcct caaggccgat gagtttctgg cactgcccga agagaagaag ctggccgtcg
     1981 cgcgcatcat cgcccgcccc cgcatcatca ccctgcccgg ctcacccgcc aagatctcga
     2041 tcaattctga ttctgacgac accgacaaaa atgcgcggtt tcacaagatc aacctgaccg
     2101 ccgatttact gcccctgccc gacggcatgg tgcgtttcaa gaccgggttc ttcatcgaac
     2161 tgaagtcgaa agacgccaag ggaatcaagg aaaccaagcg ccagggtgaa tcaacctacg
     2221 tcgtgaaacc gggcgccacc ttcggtcagg acctcgaaac cgaaaacggc accgttctta
     2281 tcgagtatac cgtccgccag gccgagtaag tcgtcaacat cagaccgggc gggccgcaat
     2341 cggcccgccc ggtctgatgt tgacaaccac ctattttgta tatacattat gactatgccc
     2401 tacgagtggg
//
LOCUS       DAORHD010000789         2409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53472_length_2409_cov_3.973662, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000789 DAORHD010000000
VERSION     DAORHD010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53472_length_2409_cov_3.973662"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..>2409
                     /locus_tag="PKO06_15460"
     CDS             32..>2409
                     /locus_tag="PKO06_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71099.1"
                     /translation="MTPEIWEASPAGSGNAVIQSSATPASATVRCSHPVSFQASYIPV
                     TVTAPGSWFYLHGAGTPTRLASGTSLADATPWPVSDSLSTGIRIYEEKLTVTPTPLHL
                     GTVNPGSLSAPKNLTVTNTGNLDLEHVDWEMHNLSTDAYFIPYNAILMNPDPVGGLAI
                     ATAMTCSASVLVPPATPPGTYSALQFAFEDNDQNGNSMEEAIGQFDMQVHVPSVQKLQ
                     VGTTSIFLGSWLANDTTATHTALISNIGNRSLSVVRLLVSDFLLNGTDATRRIASSNV
                     QLNPIAIGYMVAGESRWQALSVKVIPSPLAATGTFIATGTFFNDADADAQVDPGEASA
                     TMQISLIIGPSESFTVTPVPVNLGTRLPGELTVSSVVYASNTGGLPLENLRFEVATMS
                     ETTGDVISPQYISMAPDPLPVVGIGASTSFQVFINIPFGAKESVGGHDYGEQLPQSYQ
                     YLYNDKNKNGIRDTGEAATPFQCLVKVGITASFTVSEEIANFGTGLPGETLTTTVDVI
                     SIGNDKCQKMKWLVTGNSLTSGPNSIPFTPGAATIEPYEAAGWLLDVPPATTHQLATL
                     TLAIPLGTPVGVYTGRCRLYNDTIGPPWNTYNAGEPTDEFDLRVEVGARGLDILDSSP
                     FPLGIGIPASETAPAIFSVKNTGQRTLNDLKYQKPTLTSGGNTIPAANLLYSLPDPIG
                     LLNAGAIWAPSVRVSVPPNQEAGIYADTLYVYQDDDHDGIMDAVEIRDSLDFTLEVQL
                     KRILQFAPSPLDMGGINKGQTVTRTVLATNLGNRPLNTLCWEIPDLKDGGNLL"
BASE COUNT          502 a          790 c          628 g          489 t
ORIGIN      
        1 cggaacttct taccttcacg aggagatcgt cgtgactccc gagatctggg aagccagtcc
       61 ggccggttct ggaaatgcgg tcatccagtc cagcgccaca ccggcgagcg ccactgtccg
      121 ttgcagtcac ccggtctcct ttcaggccag ttatattcca gtcacggtga cagcccccgg
      181 aagctggttc tatctgcacg gagctgggac tcccacaagg ctcgcatccg gcacctctct
      241 cgccgatgcc accccctggc ctgtgagcga cagtctctcg acgggcattc ggatctacga
      301 agagaagctc acggtcacgc cgaccccctt gcatctgggc acggtcaatc cggggtcgct
      361 cagcgctccg aagaatctga cggtgacgaa cacagggaat ctcgatctcg agcacgtcga
      421 ctgggaaatg cacaacctgt ctaccgatgc gtatttcatt ccatataatg ccattctgat
      481 gaaccctgac ccggtcggtg gcctcgccat tgcaaccgcg atgacctgtt ctgcttcggt
      541 acttgtccct ccggccaccc cgcccggcac ctattccgct ctgcagtttg cctttgaaga
      601 caacgatcag aacggaaact ccatggaaga ggccatcggc cagttcgaca tgcaggtgca
      661 cgtgccttca gtccagaaat tgcaggtcgg caccaccagt atcttcctcg gcagctggct
      721 ggcgaacgac acgaccgcca ctcacacggc gctcatcagc aacattggaa atcgcagtct
      781 ctccgtggtg cgcctgctgg tttcagactt tctgctcaat ggcactgatg ccaccagacg
      841 catcgcgagc agcaacgtgc agctgaaccc catagccatc ggctacatgg tcgccggcga
      901 aagccggtgg caagcgctct cggtgaaggt catcccgtca cctctggcag ccaccggaac
      961 cttcatcgcc acgggcacgt tcttcaacga cgcagatgcc gatgcccagg tcgaccccgg
     1021 tgaagcctcg gcaaccatgc agatctctct gatcatcggc cccagcgaga gttttacggt
     1081 cacgccggtc cccgtgaatc tcggcacccg ccttcccgga gaattgacgg tttcctccgt
     1141 cgtctatgcc tcaaacacag gcggtctgcc ccttgagaat ctgcgcttcg aagtcgcgac
     1201 catgtccgaa accaccggcg acgtcatctc gccgcagtat atctccatgg ctcccgaccc
     1261 gcttcctgtc gtcggtatcg gtgcttccac ctctttccag gtctttatca acatcccgtt
     1321 cggcgcgaaa gagtcggtcg gaggtcatga ttacggcgag caactgccgc aaagctacca
     1381 gtacctatac aatgacaaaa ataagaacgg gattcgcgac accggtgaag cggccactcc
     1441 gtttcagtgt ctcgtcaagg tcggaatcac ggcatctttc acagtctccg aagagatcgc
     1501 caattttggc accggcctgc cgggagaaac actgacgacc accgtcgatg tcatcagcat
     1561 cggaaacgat aagtgccaga agatgaaatg gctggtgacc ggcaactcgt taacctcggg
     1621 tccgaactcc attccgttta cgccgggtgc agcaaccatc gaaccgtatg aggccgccgg
     1681 gtggctgctc gacgttcctc cggccaccac gcatcaactc gccactctga ccctggccat
     1741 tccgctcgga acgcccgtcg gtgtctatac aggtcgttgc cgtctctaca acgacacgat
     1801 cggcccccca tggaacacct ataatgccgg ggaaccaaca gacgagtttg atttgcgtgt
     1861 tgaagtcggc gcccgtggtc ttgacatcct ggattcgtca ccctttccgc tgggaatcgg
     1921 catccccgcc tctgagaccg cgcccgcgat tttctcggtg aaaaacacgg gacaacgcac
     1981 gctcaacgat ctgaagtatc agaagcccac gctgaccagc ggcggcaaca cgattcccgc
     2041 cgccaatttg ctctactcgc ttcccgaccc gatcggcctg ctgaacgcgg gggcgatctg
     2101 ggctcccagc gtccgcgtct ccgttccgcc gaatcaggaa gccggcatct acgcagacac
     2161 cctgtatgtc tatcaggacg atgatcacga cggaatcatg gatgcggtcg agattcgcga
     2221 cagtctggat tttacgcttg aggtgcagtt gaaacgtatt ctgcagttcg cgccttctcc
     2281 acttgacatg ggcggcatca acaaggggca gacggtcact cgcaccgtac tcgccaccaa
     2341 cctgggaaat cggcctttga acacgctctg ctgggagatt cccgatctga aagatggcgg
     2401 gaacctcct
//
LOCUS       DAORHD010000790         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53558_length_2407_cov_3.650935, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000790 DAORHD010000000
VERSION     DAORHD010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53558_length_2407_cov_3.650935"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKO06_15465"
     CDS             <1..436
                     /locus_tag="PKO06_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71100.1"
                     /translation="GVKSSGGAAVRVVDGYRLLFFAFGIEAVNSQVQRAALMKEIVTF
                     MTPEIGRQIQDLAGASRNRGRGRARTSRDVALSAEMMGNLQERILKQVKQNLEANPQT
                     TVRALEMIRALPSDSRAAVSSLERDLQGMVNFSRMQEPAPQR"
     gene            621..1010
                     /gene="ftsL"
                     /locus_tag="PKO06_15470"
     CDS             621..1010
                     /gene="ftsL"
                     /locus_tag="PKO06_15470"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNV71101.1"
                     /translation="MSPLKVTNVSLPRGSDHPGKTEALPVALSRRRRLVRLYLTAMIL
                     LSLMFTLYVWQSTKIVEVKFRLQQLEKKADALETNNSVLKADISRLQSLSRIERVAKQ
                     DLGMVVPSRLCYVPIPPHLMHTGAGAP"
     gene            1007..1939
                     /gene="rsmH"
                     /locus_tag="PKO06_15475"
     CDS             1007..1939
                     /gene="rsmH"
                     /locus_tag="PKO06_15475"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV71102.1"
                     /translation="MTWTHAPVLLDPILNLGVSADCRTFVDLTFGLGGHAQAILQRFP
                     DIRLYIGVDRDREILQLTDSEPLDPRIRRLGGRASDVESHLSACGLVEADDVLMDLGV
                     CSVHFDNRERGFSFQGGPLDMRLERTMAVSAREVVNEFAERDLAHILKQFGEEPFARQ
                     IARHIVRRRAAAPIETNADLAAIVEAAIPVRIRARMTVHPATRTFQAIRIQVNDELQE
                     LEAALEAIYRVLRPGGRMSIISFHSLEDRIVKQFLVRKAKGCVCPPHFPVCACGFRPQ
                     LKILTRKPMEADESEIRQNPRSRSAKLRIAEKLP"
     gene            1936..>2407
                     /locus_tag="PKO06_15480"
     CDS             1936..>2407
                     /locus_tag="PKO06_15480"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71103.1"
                     /translation="MKNVLLRSKTMRAGSLFFGITIGLCLFIARLVQLQVVQHETWAG
                     RSERNHQNKRVLEMKRGAIYDRNGQELAISVETYTVNVYTREIKVMSEVANTLSTVLP
                     MTRQEILEKIGTRKGYIPVYKNLERSQANKLMTMNLPGVILEENYRRVYPQNNLA"
BASE COUNT          519 a          615 c          722 g          551 t
ORIGIN      
        1 cggcgtaaaa tccagtggtg gagccgcagt tcgagttgtc gatggctatc gcctgctgtt
       61 cttcgcgttt ggaattgaag ccgtgaactc gcaggtgcag cgtgccgcgt tgatgaagga
      121 gatcgtgacc ttcatgactc cagaaatcgg cagacagatt caagatctgg cgggtgcctc
      181 gcgtaatcgg gggcgcggtc gggcgcggac ctctcgcgat gtggctctga gcgcggaaat
      241 gatgggcaac ttgcaggagc ggattctgaa gcaggtgaag cagaatctgg aggcgaatcc
      301 ccagacgacg gttcgagcgc ttgagatgat ccgcgcactg ccctcagaca gccgggcggc
      361 tgtttcctcg ctggaacgag atctgcaggg aatggtgaac ttctctcgga tgcaggagcc
      421 ggctccgcag cgctgattcc cacgtcttga cgaaaaatca gggagaaacc ctcgtggttt
      481 ctccctgcct cttgtaccac gaaagaaaat ggcgtatatt cctgaatgag tcgggccagg
      541 tgtcttcgcg acattgccgt gctcgaacgg tacgagtgcc ctgactcgaa aaagctctcg
      601 agaaaagcgt taggtgctgt atgtctcccc ttaaagtaac aaacgtcagc ttaccccgtg
      661 gaagtgacca cccggggaag accgaggctc tgccggtggc attgagtcgt cgtcggcggt
      721 tggttcggtt gtacctgacg gcgatgattc tgctgagcct gatgtttacg ctctacgtct
      781 ggcagtcgac gaaaatcgtc gaagtgaaat tccgactgca gcagcttgag aagaaagctg
      841 acgcgttgga aaccaacaac agtgttctga aggcggatat ttcgcgttta cagtccctgt
      901 cgcgcattga gcgcgtggcc aagcaggatc tgggcatggt ggtgccgagt cggttgtgct
      961 atgttcctat tccaccgcat ctcatgcaca cgggtgcagg tgccccatga cctggactca
     1021 cgctcctgtg ctgctcgacc cgattttgaa tctcggagtc agcgccgatt gccgaacgtt
     1081 tgtagatctg acgtttggct tgggaggaca tgctcaggcg attctacagc ggtttcccga
     1141 cattcgactc tacatcgggg tcgatcgtga ccgtgagatt ctgcagttga ccgattccga
     1201 acccctggat ccgcggattc gtcgcctcgg cggccgcgcg tctgacgtgg aatcgcatct
     1261 ttcggcttgt ggtctggttg aggccgatga tgtgctgatg gatcttggag tttgttcggt
     1321 acatttcgat aaccgcgagc gcggcttctc atttcagggc ggtcctttgg acatgcgtct
     1381 cgaacggacg atggcggtga gtgcgcgcga ggtggtcaat gagtttgccg aacgtgatct
     1441 ggcgcacatc ttgaaacagt tcggagaaga gcccttcgcg cgtcagattg cgcgccatat
     1501 cgtccggcga cgcgcagccg cgccgatcga gacgaacgct gacctggcgg cgatcgtcga
     1561 agcggccatt cctgtcagaa ttcgtgcgcg tatgaccgtg catcccgcaa cgcgaacgtt
     1621 tcaagcaata cgcattcagg tgaatgacga actccaggaa ctggaagcgg cactcgaggc
     1681 aatctaccga gttcttcgtc cgggtggtcg catgagcatc atttcgtttc actctctgga
     1741 agatcgtatc gtgaaacagt ttctggtacg gaaggcaaaa ggttgtgtgt gccctccgca
     1801 ttttcccgtg tgcgcctgcg gcttcagacc gcaattgaag attctgacca gaaagccgat
     1861 ggaggcggat gaaagtgaga ttcggcagaa cccgcgctcg cgatcggcca aactgcggat
     1921 tgcggagaag ctgccatgaa gaatgtgctg ctccgttcca aaaccatgcg ggctggttca
     1981 ctcttctttg gcatcactat tggtttgtgt ttgtttatcg cgagacttgt acaattgcag
     2041 gtcgtgcagc acgagacctg ggccggacgt agcgagagga accatcagaa taaacgcgtt
     2101 ctggaaatga aacgcggcgc catttatgat cggaatgggc aggaactggc gatttcggtc
     2161 gaaacctaca ccgtgaacgt gtatacccgc gaaatcaagg tcatgagtga agtagccaat
     2221 acgctgtcga cggtcttgcc gatgacacga caggaaattc tcgagaagat tggcactcgc
     2281 aaagggtaca ttccggttta caagaatctt gagcgatctc aggccaacaa gttgatgacc
     2341 atgaatctgc ccggcgtgat tcttgaggaa aactatcgcc gagtgtatcc gcagaataat
     2401 ctcgcct
//
LOCUS       DAORHD010000791         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53673_length_2404_cov_3.754789, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000791 DAORHD010000000
VERSION     DAORHD010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53673_length_2404_cov_3.754789"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PKO06_15485"
     CDS             <1..684
                     /locus_tag="PKO06_15485"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HNV71104.1"
                     /translation="SDMMWVTSRFGVADCKKLTNGAMQPYIIDVACVNGNFVKNAECF
                     AEAWMRAGTIEAPAGAIGICAASTNMEWVPPCIVQAEMSSVYIAKNEYKTAGGILMNG
                     IIKGLEQYGTSEKGSGVMMYEQWHLFGDATLNVRTNAPVALRAEGKASRADGVVTAEV
                     VVVDAAGTPIRDARVAVYTEGMGTMGSTMTNAEGKAIVTISADAETPVYYTITGGDLV
                     PVVDQKLTF"
     gene            complement(754..>2404)
                     /locus_tag="PKO06_15490"
     CDS             complement(754..>2404)
                     /locus_tag="PKO06_15490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71105.1"
                     /translation="TGVDSCLVYQFKAGEEAFVCLSQWTIDGAPALDPAHQRLPRAFI
                     PHISRMLEVEEDVAVFSAAYLPDEAVKDREFLNRAGIKSFLAVPMSYNKELLGFLALL
                     NLRQPREWKHQHISLLHILGEIFVNALVRQNSEEELTRVREQEVGIASRIQQSLLLGA
                     IPETLSNAHIAGLTIPSMKVDGDFYDLIQHSPECFDLLIGDVMGKGVPAALMGAATKS
                     QFLRAIVRLIPASAPGTFPAPEAIVTSVHHEMTAQLLELESFVTLCYARFDLASQELT
                     LVNCGHPRALHFTAETGICSSPSANNMPLGISEHATYSQVVLPFARGDLFLFYSDGIT
                     EARNSDRQPFGDIRLAQVIEKHAPGHSPEELVEKIRSQVVEFCQDEHFGDDLTCLAVK
                     IKEIEPHHALFKSELEIPSHPEELALARTWIKEFWVQVTGRSADDLECMALLMALNEA
                     LANIIKHAYHNRHDKRILLEVEGFVDNIVLRMYHQGDPYIPGSAPPPSFDGSRENGFG
                     LYLIKHSVDEVCFFRDGHGRHTIRLEKRFKPRPAPEPNSDT"
BASE COUNT          507 a          664 c          748 g          485 t
ORIGIN      
        1 agtgacatga tgtgggtgac cagccgcttc ggcgtcgctg actgcaagaa gctcaccaat
       61 ggcgcgatgc agccctacat catcgatgtc gcctgcgtga acggcaactt cgtgaagaac
      121 gccgagtgct tcgccgaggc ctggatgcgt gccggcacga tcgaagctcc ggccggtgcc
      181 attggcatct gcgctgcctc gaccaacatg gaatgggtgc ctccgtgcat cgttcaggcc
      241 gaaatgagca gtgtctacat cgccaagaac gagtacaaaa ccgccggtgg cattctcatg
      301 aatggcatca tcaaaggtct cgaacagtat gggaccagcg agaagggcag cggcgtcatg
      361 atgtatgagc agtggcacct gttcggtgat gcgacgctca atgtgcgcac caacgctccg
      421 gtggccctgc gcgccgaagg caaggccagc cgcgctgatg gcgtggttac tgccgaagtc
      481 gtggtggttg atgcggccgg caccccgatt cgcgatgcgc gtgtcgcggt ctacaccgaa
      541 ggtatgggca ccatgggctc gaccatgacc aatgccgaag gcaaggcgat cgtcacgatc
      601 agcgccgatg ccgaaacccc ggtttactac accatcacgg gtggagatct ggttccggtt
      661 gttgatcaga agttgacgtt ctaagcattt ccgcaaaaaa gcccggccat ctggccgggc
      721 ttttttgcgt acctcgggaa tcgactcgac tcttcaggtg tccgagttcg gctcgggggc
      781 tggccgaggt ttgaagcgct tttcgagccg aatggtgtga cgaccgtgac catcgcggaa
      841 aaagcacacc tcgtcgaccg agtgcttgat gagatagagt ccgaatccgt tttctctgga
      901 accatcaaat gatgggggcg gagcagaccc gggaatgtag gggtcgccct ggtgatacat
      961 gcgcaggacg atattgtcga cgaatccttc gacttcgagc agaatgcgct tgtcgtgccg
     1021 attgtgatac gcatgcttga tgatgttggc cagagcctca ttgagcgcca tgagcagcgc
     1081 catgcactcg agatcatcgg ctgatcgacc cgtgacctga acccagaatt ccttgatcca
     1141 ggtgcgggcc agggccagtt cttcggggtg gctcggaatc tcgagttcgc tcttgaacag
     1201 cgcgtgatgc ggctcgatct ccttgatctt gactgccaga caggtcaggt catcgccaaa
     1261 atgctcgtcc tgacagaact cgaccacctg gctgcgaatc ttctcgacca gttcttcggg
     1321 cgaatggccg ggagcgtgct tttcgatgac ctgagccagg cggatatcgc cgaagggctg
     1381 tcgatcgctg ttgcgtgctt ccgtgatgcc gtcggagtaa aatagaaaca ggtcgccgcg
     1441 cgcgaagggg agaaccacct gcgagtaggt ggcgtgctcg ctgatgccga gaggcatgtt
     1501 gttggcgctc ggactggagc agatacccgt ttcggcggtg aaatgcaggg cgcgcggatg
     1561 tccgcagttg accagtgtga gctcctgact ggccagatca aagcgggcgt aacagagagt
     1621 gacgaagctc tcgagttcga gcagctgcgc cgtcatttcg tgatgaacac tggtgacgat
     1681 ggcttccggg gccggaaaag tgccgggcgc agaggccgga atcaaccgca cgatggcgcg
     1741 tagaaactga cttttcgtgg cggcacccat gagcgcggcc ggaacccctt tgcccatgac
     1801 gtcgccgatc agcagatcga aacattcggg cgaatgttga atcagatcgt agaaatctcc
     1861 gtcgaccttc atcgagggaa tcgtcagacc ggcgatgtga gcgttcgaga gagtctcggg
     1921 aatcgcgccc aacagcagcg actgctgaat gcgagaagcg atgccgactt cctgctcgcg
     1981 gacgcgcgtc agttcttctt cactattctg gcgcaccagg gcgttcacga agatctcgcc
     2041 gagaatatgc agcagactga tgtgctgatg cttccattcg cggggctggc gtagattcag
     2101 cagcgcgaga aacccgagca gctctttatt atacgacatc ggcaccgcca agaacgactt
     2161 gatgccggca cgattcagga actcccggtc tttgaccgct tcgtccggaa gataggcggc
     2221 ggaaaagacc gcgacgtctt cttcgacttc gagcatgcgt gagatatggg gaatgaacgc
     2281 tcgcggcagt cgctgatgag ccgggtcaag agccggtgcg ccatcgatgg tccattgact
     2341 caggcacaca aaggcctctt cgcccgcttt gaactggtac accagacaac tgtcgacgcc
     2401 cgtg
//
LOCUS       DAORHD010000792         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53680_length_2404_cov_3.348659, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000792 DAORHD010000000
VERSION     DAORHD010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53680_length_2404_cov_3.348659"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKO06_15495"
     CDS             complement(<1..682)
                     /locus_tag="PKO06_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71106.1"
                     /translation="MSIVSRVMVWFAGWSRPAKAFLGLALLLYIPLLLLRQLLGMAIT
                     LDETKVREDYQERIQLEMQSFVGDAVADSQVKKAFNEVLEKLPIATGTALDEAWVGRF
                     LPLLKQAVPFEPSDLWVFGPDLKCLHLEGTPSFMREKARWIFAALHRNLWDVCRRDED
                     FRQTVLRNQGLNEYQSQLFVEAINEGGMLLDRARSLAKELWSEREDVHKMMQDMDEDD
                     PESILSEIE"
     gene            complement(696..1253)
                     /locus_tag="PKO06_15500"
     CDS             complement(696..1253)
                     /locus_tag="PKO06_15500"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNV71107.1"
                     /translation="MNSGKLTVIVGPMFSGKTSELISFVEIYALGRKKHQVFKPALDN
                     RYDASRIVSHTQAKVDARAVRNPGEILALLHGDEKAVFVDEIQFLDLSLRDVILTLIE
                     RGVNVYCSGLDLDFRNQPFPTTMALMAHADEVLKKRAVCHECGEYAGTLSHKLVQDQQ
                     VIDVGGEDKYIATCRTCYRRLHKAS"
     gene            1823..2281
                     /locus_tag="PKO06_15505"
     CDS             1823..2281
                     /locus_tag="PKO06_15505"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV71108.1"
                     /translation="MSTPAVMIGQQLMECFHQLLRLVRSRIEPRGLTGPQFGILRHLA
                     QAGPLTPTDLSQRLIVTPGNITGPLQRLVGLKLIEMRRSPKDRRKIHVAITQAGRQKV
                     AEITPQLEDLLQSCLNPISTADQREFTRLLTLLNQQLHSATIHLKGSHPE"
BASE COUNT          549 a          747 c          598 g          510 t
ORIGIN      
        1 cctcgatctc ggagaggatg ctttccggat cgtcctcgtc catgtcctgc atcatcttgt
       61 gaacgtcttc gcgctcgctc cacaactctt tggcaaggct tcttgcccga tcgaggagca
      121 tccctccctc gttgatcgct tcgacgaaca gctgactctg atactcattc agtccctgat
      181 tgcgcagtac cgtctgacga aaatcttcat cacgacggca gacatcccac aggtttcggt
      241 gaagagccgc gaagatccag cgagcttttt cccgcatgaa ggagggggtg ccctcgagat
      301 gcagacattt caggtccggg ccgaagaccc agaggtctga cggctcgaac ggaaccgcct
      361 gcttcagcaa cgggagaaat cgcccgaccc aggcttcgtc caacgcagtg ccggtcgcga
      421 tgggaagctt ctcgagcacc tcgttgaacg ccttcttcac ctgcgaatcc gcgacggcgt
      481 cgccgacgaa actctgcatc tcgagctgaa tacgttcctg gtagtcctcg cgcaccttgg
      541 tttcgtcgag cgtgatcgcc atgcccagca gttgccgcaa cagcaacaac ggaatgtaga
      601 gcaacaacgc gagtccgaga aaggctttcg ccggacggga ccatccggcg aaccacacca
      661 tcactcggct cacgatcgac atgcctcaga ttcttttacg atgccttatg gagacgtcgg
      721 tagcaggtac gacaggtggc gatatacttg tcttcaccgc cgacatcgat cacctgttga
      781 tcctgcacca acttgtgcga cagagtgccc gcatactcgc cgcactcatg gcagacggct
      841 cgcttcttga gcacttcatc ggcgtgagcc atcagggcca tcgtggttgg gaacggctga
      901 ttgcgaaaat caagatcgag accggagcag tagacgttca cgccgcgctc gatcagcgtg
      961 agaatgacat cgcgcagcga gagatcgaga aactgaatct cgtccacgaa caccgctttc
     1021 tcgtcgccat gcagcagggc cagaatctct ccggggttgc ggaccgcccg ggcatcgacc
     1081 ttcgcctgcg tgtgtgacac gatacgggag gcatcgtacc ggttatccaa agccggcttg
     1141 aagacctggt gcttctttcg accaagggca tagatttcta cgaacgagat cagctctgag
     1201 gtcttgcccg aaaacatcgg gcccacaatc acagtcagtt ttcctgaatt cattgcacca
     1261 tctccttgaa tcttcagcac ctgtgacaca tcacggccga ggcagcggcc catgacgaga
     1321 acacccaaaa gtatatgatg ctttggtaac ggaccctgaa tacgagccac cttcagggca
     1381 gacagggatc tctttcaaat attgttgcgt gcccaggaac aacaggacat gccgatcgat
     1441 gcggttgata gaaacctcat gatccatatt gtacatttcg accgccccca gaaacgccct
     1501 ctgattcgca acacagctct gcctgggtgt ccgaccggct gacttccgat actcgataag
     1561 agaggggaac aggtgcagtg gtgttccggt ccacagagca caacagctca gcgccagaat
     1621 caccagtacc agtatcacac cacagccact cgttttcgtc tcgttcatat cccttttcgc
     1681 gtcacacgcg gtcgccagta taccgcaact tctctcacgg gaaagtcgtc tcgaaacgac
     1741 tcatcacagg cttgacagca cgagcaagac ttggtagtat cataattact ttatgcctta
     1801 actatttagg aataaacgat tcatgtccac acctgccgtc atgatcggac aacagctcat
     1861 ggagtgcttc caccaactcc tgcgcctggt gcggtcccgt atcgaacccc ggggtctgac
     1921 cgggccgcag ttcgggatcc ttcgccatct cgctcaggcc ggccccctga cacccaccga
     1981 tctgagccag cggctgattg tgactcctgg caacatcacc gggcccctgc agcgactcgt
     2041 gggtctcaaa ctgatcgaaa tgagacgttc tcccaaggat cgacgaaaga ttcacgtcgc
     2101 gatcacacag gcggggcgac agaaagtcgc cgagatcacg ccgcagctcg aagacctgtt
     2161 acaaagctgt ctgaatccca tttctaccgc cgatcagcgc gaattcacgc gcctgctcac
     2221 gcttctgaat cagcagttgc attctgccac cattcacttg aaaggcagcc accccgaatg
     2281 aaatcactct gtcatctcac gtgctccatc attcttctct ttctcgccct ggtgaccgtg
     2341 cccgcgatga gcttcagcga ggctcaagat gaaacaccca caaccttgtc gcttgagcaa
     2401 tgcc
//
LOCUS       DAORHD010000793         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53751_length_2402_cov_3.613975, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000793 DAORHD010000000
VERSION     DAORHD010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53751_length_2402_cov_3.613975"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKO06_15510"
     CDS             <1..943
                     /locus_tag="PKO06_15510"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HNV71109.1"
                     /translation="TEADVAEALGISVGQLRHFSIHRERERVCHYVRFAIPKRTGGER
                     VIMAPKKRMKGLLRQLNRDLVQKLPVHEAARGFVPGGSVRANAEPHVGRKYVIRLDIR
                     DFFPTLHFGRVRGYLIAVGFGYAVATILAVLMTEAERQPVQIDNQVYQVPIGSRYCVQ
                     GAPTSPGLANAIFKRADRRLSQLAAKLEMNYSRYADDLTFSGDDESVVVELFRYAMLI
                     LTNEGFTLNLAKTRVMRASNAQRVTGATVNQVLGLSKKERKRLRAALHQNALAPDKSR
                     EPRTTLLGRLAWVQALNPTQAEVLRRDYERHHRSRPL"
     gene            complement(1030..1575)
                     /locus_tag="PKO06_15515"
     CDS             complement(1030..1575)
                     /locus_tag="PKO06_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71110.1"
                     /translation="MLQERSQRRGLSVVELVFGFTIFALLLLIAGRLISSANRQTDVV
                     SGYATFHLNAGRLIEAMKLDLRSAIEIDVGTASLQIERVLGVASDGQILRETVSYAEA
                     GTHVQERRAGRDRAHILCPDSKSVTRIRLQFEVAHRHEELGEPRGLLKLTVSAFSSDF
                     EAHERYVASAAVEIKPRVLRP"
     gene            1876..2355
                     /locus_tag="PKO06_15520"
     CDS             1876..2355
                     /locus_tag="PKO06_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71111.1"
                     /translation="MTESRRRKGISVLESIIAVLVFAIVFIPLYRLYTETGVEQQKMV
                     RDFAVAINIAENALSLIESEIDKGSLPNPVTEQDVSSLFLGNPNTQKAIQGMLGYGAA
                     ESVKYIPAFRLFLTTRPYKGNANLYEIRLRFVWGSQKTGNSIEEKHEFELKTLRTCM"
BASE COUNT          564 a          674 c          664 g          500 t
ORIGIN      
        1 gaccgaagcg gatgtagcag aagccctcgg catcagcgtg ggtcagttgc gccacttctc
       61 gattcatcgc gaacgtgagc gggtctgcca ctacgtgcgg tttgcgattc ccaaacgtac
      121 cggcggtgaa cgtgtcatca tggctccgaa aaagcgtatg aagggtctgc ttcgccaact
      181 caatcgtgac ctggtgcaga aactaccggt tcatgaagcc gcgcgtggat ttgttcccgg
      241 cggatcggtg cgcgccaacg cggaaccgca tgtcgggaga aagtacgtca tccggctcga
      301 catccgcgac ttctttccga ccctgcattt cgggcgagtg cgcggctatc tgatcgccgt
      361 cgggtttgga tatgccgtcg ccaccatact ggcagtactg atgaccgaag ccgagagaca
      421 accggtacaa atcgacaatc aggtgtatca ggtgccgatc ggctcgcgct actgcgtaca
      481 gggggcgccg acgagccccg gcttggcgaa tgccatcttc aaacgagccg atcggcgttt
      541 gtcccagttg gccgcaaagc ttgaaatgaa ttattcaagg tatgccgacg acctgacttt
      601 ctcaggtgat gacgaatcgg tggtggttga gctctttcgg tatgccatgc tgattctcac
      661 caacgagggc ttcacgttga acctggcgaa gacccgggtg atgcgtgcct caaacgcaca
      721 acgggtgacc ggggcaaccg tcaaccaggt tctgggcctt tcgaagaaag agcgcaaacg
      781 ccttcgcgct gccctgcacc agaacgccct cgcccccgac aaatcccgcg aaccgcgcac
      841 tacgctgctc ggccgcctcg cgtgggtgca ggctctgaac ccgacgcaag ccgaggtctt
      901 gcgccgcgac tacgaacgtc atcacagaag cagaccgttg tagagaagct caactgagct
      961 tcttcgacag gggaaaacgt tgtccaggca tctgtgttct gactgttcga acggccctga
     1021 acaagtcaat caggggcgaa gcacgcgagg tttgatctcg acggccgcag aggcgacata
     1081 gcgctcgtgg gcctcgaagt cgctgctgaa agcactgaca gtcagtttca gaagtccgcg
     1141 aggctcaccc agttcttcgt gtcgatgagc aacctcaaac tgcagtctga tacgagtgac
     1201 gcttttcgaa tcgggacaga gaatatgggc ccggtcgcgt ccggcccggc gctcctgaac
     1261 atgagttcca gcttcggcat aactcacggt ctcacgcaaa atctgaccgt ctgacgcaac
     1321 accgaggact cgttcgatct gcaggctcgc ggtaccaacg tcgatctcga tagccgaacg
     1381 caggtcaagc ttcatggctt caatgaggcg tccggcgttc aagtgaaacg tcgcgtatcc
     1441 ggagaccacg tctgtctgcc gatttgcaga ggaaatcaaa cggccggcga tgagcagcaa
     1501 caaggcgaaa atcgtgaagc cgaacaccag ttccaccacc gacagaccgc gtctctgact
     1561 gcgttcctga agcatcccgc gccaccttcg ctcaactgag atgacaattg ttgtcacatt
     1621 cccgaggtcc atggtgtcag aaaagaagtg tcatgacaca agcttcagga aggagcttca
     1681 aagcctggta ctccatcttc cttctttcac gctcgaagaa tttcttctcc cacaagtctt
     1741 gaccatacta cgccgttttc ctgacgggtg agaaacatcg catatctctg gaaaaagcca
     1801 gcgagatacc gtatcattga acgggaaagt tctgttcgat agaggcgaag attcaccgaa
     1861 cgaggatgcg aatacatgac agaaagcaga aggcgtaaag gaatcagcgt cctcgagagc
     1921 attatcgcgg ttctggtgtt tgccatcgtc ttcattccac tctatcgctt gtacacggag
     1981 accggggtcg aacagcagaa aatggtgcgt gactttgcgg tggctatcaa cattgctgag
     2041 aatgctctga gcttgattga gagcgagatc gacaagggca gtctgcccaa cccggtcacg
     2101 gaacaggatg tttcttcgct attcctcggg aatcccaaca cccagaaggc cattcagggc
     2161 atgctcggtt atggggctgc cgaatccgtg aagtacatac ccgcctttcg cctcttcctg
     2221 accacacgcc cctataaagg caatgccaat ttgtacgaaa ttcgtctgcg gtttgtctgg
     2281 ggcagtcaga aaacggggaa cagcatcgaa gaaaagcacg agttcgaatt gaagacgctg
     2341 aggacctgca tgtgatcatt gcttcacgca aactggggtt taccgtcgtc gaagtgatcg
     2401 tc
//
LOCUS       DAORHD010000794         2402 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53758_length_2402_cov_3.089050, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000794 DAORHD010000000
VERSION     DAORHD010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53758_length_2402_cov_3.089050"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKO06_15525"
     CDS             complement(<1..1099)
                     /locus_tag="PKO06_15525"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV71112.1"
                     /translation="MSRSTFVALRRLVVLLPLLLLVGFAQPLEALSRCPGCQKLFPEN
                     HRFCPYDGKALVVLKSEELGRVEVQISPAQATIQCGSLSGKGPVWVAELPPGDHQITA
                     ETPGFQSQKMRVSLEKGQTVKLSLELEALATGKSTPTLPESLANAPAQTNGTAWNRPQ
                     GRDADMIEVRGMPYLLGSERGNPDERPMRRVELEPFWIDRHEVTCAQYQKFLEAMRRS
                     GHRWCHPNEPPNKDHTPFHTYAWALRFSWVGGKPPADMLDHPVVLVDWFDAYAYANWA
                     GKRLPSENEWEAAAGNGLGRDYPWGNTFGPKWCNTGDYPLRVGLYPAGASPVGVLDMA
                     GNVWEWTATGYDVDPREARSFDGRHGSVIIRG"
     gene            1316..>2402
                     /locus_tag="PKO06_15530"
     CDS             1316..>2402
                     /locus_tag="PKO06_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71113.1"
                     /translation="MKNASTVAGWVIFVLLPPLLMAWALDTSLTLNRESELRRSREFL
                     SRFARTTLAQATPRTFVRRAFQQGTPPTWSAPALAFEAGRPGSGSTPLLDEISGHLQA
                     GTFPTPERYDVWRHQIGWEFDFHRFRSHPGVPQAVRWGRQEAWLVWKPLATTSVRLAL
                     YVVPPLVERILTSMPEHAMEQRLAWAIAYPEAKRWRARAPFRPRVIRPMLTALKGGVA
                     TTRRTATHLYYAESLEQNLVLMLQKPFSGWYVHTTRRLLWSFCLGLLAALSIAYPYLV
                     RRVPAWPLKIKFLGIFAYLVGLPLAGVAIFAMAVTSDRLHLKQTELRRLARSELLRFD
                     DGLREEIRATQLFFRSIPNHPALQQRRL"
BASE COUNT          439 a          784 c          697 g          482 t
ORIGIN      
        1 cgccgcgaat aatcacgctg ccgtgccgac catcgaatga tcgggcttcg cgcggatcga
       61 catcgtagcc ggtcgccgtc cattcccaga cgttgccggc catgtcgagc acacccaccg
      121 gcgacgcacc ggcgggatac agaccaacgc ggagaggata atcgccggtg ttacaccact
      181 tcggcccaaa ggtgttcccc catggataat cacgaccgag cccattcccg gcggcagctt
      241 cccactcgtt ttcggacgga agtcgcttgc ctgcccagtt ggcatacgca taggcatcga
      301 accaatccac cagtacgacg ggatgatcga gcatgtcggc cggtggcttc cccccgaccc
      361 agctgaaccg cagcgcccac gcgtaagtat ggaaaggcgt gtggtccttg ttaggcggtt
      421 cattcgggtg gcaccagcga tgcccgctgc gacgcatggc ttcgagaaac ttctgatact
      481 gagcgcaggt cacttcgtgg cgatcgatcc agaagggttc gagctcgact cgtcgcatgg
      541 ggcgttcgtc cgggttgccg cgctcactgc ccaacaaata aggcatgccg cgcacctcga
      601 tcatgtccgc atctcgaccc tgcggtcgat tccaggctgt accgttggtc tgcgccggag
      661 cgttcgccaa agactcagga agagtcgggg tgctcttgcc cgttgccaga gcctcgagct
      721 cgagcgacag cttgaccgtc tgcccctttt cgagactgac gcgcatcttc tgcgattgga
      781 acccgggagt ctcagccgta atctggtggt cgccgggcgg cagttcggca acccacacgg
      841 gacccttgcc actcagactg ccgcactgaa tggtcgcctg tgccgggctg atctgaacct
      901 cgacgcgacc cagctcctcg gacttcagca cgaccagtgc tttgccgtca tacggacaaa
      961 agcgatgatt ctcagggaag agcttctggc agccgggaca gcgactcagc gcttccaggg
     1021 gctgagcgaa accgaccagc agcagcagcg gcagcagaac cacgagccgc cgtaacgcga
     1081 cgaacgttga tcgagacatg gcgttcccct ccgatcaatg tacagcctat caggaagatt
     1141 accgccggat tcccttgtat gcaacgtatt tcgggaacat ttctcgaggg tccgccgttt
     1201 ctggtccgtc gacacgtctt ttgacaggca tggtatgccg tgctaggctt gttcacagca
     1261 ctttcgacct tgctgaaaac tgcaactcgc gtcatctcga cgcgcgaggg ggcccgtgaa
     1321 aaacgcttcg accgtcgccg ggtgggtgat ctttgttctg ttgccacccc tgctgatggc
     1381 gtgggctctc gacaccagtc tcacgctcaa ccgcgagtca gagctgcgtc ggtcacgcga
     1441 attcctgtca cggttcgccc gcaccacgct cgcccaggca acaccacgca ccttcgtccg
     1501 cagagcattc cagcagggaa caccgccgac atggtctgcg ccggcgctgg ctttcgaagc
     1561 cggtcgcccc ggatcaggct caaccccgct tctcgatgag ataagcgggc atctgcaagc
     1621 cggaaccttc ccgacccctg aacgatacga tgtctggcgc caccagatcg gctgggaatt
     1681 cgactttcac cggtttcgca gtcacccggg agttccgcag gccgtgcgct ggggcagaca
     1741 ggaagcctgg cttgtctgga aacctctggc cacgacatcc gtgcggctgg ccctctatgt
     1801 cgtgcctccg ctcgtcgaac gcattctcac cagcatgcca gagcacgcca tggaacagcg
     1861 cctcgcctgg gccatcgcat acccggaagc gaagcgctgg cgcgcgcgcg cgcccttccg
     1921 cccccgggtc attcgtccga tgctgaccgc gctgaaaggt ggcgtggcca cgacgcgacg
     1981 gacggcgacg catctgtatt atgcggagtc cctggaacag aatctggttc tcatgctgca
     2041 gaagccattt tccggctggt acgttcatac cactcgtcgc ctgctttggt cgttctgcct
     2101 tggccttctg gcggcgcttt cgatcgctta tccctatctg gtgagaaggg ttccggcctg
     2161 gccgctgaag atcaagtttc tcggcatctt cgcgtatctg gtcgggctgc cgctggcggg
     2221 agtcgccatc ttcgccatgg cggtcaccag cgatcgactg cacctcaagc agacggagtt
     2281 gcgccgcctc gctcgcagtg aattattgcg tttcgacgat ggtcttcgcg aagagatacg
     2341 tgccacccag ctgttttttc gcagcattcc gaatcatccg gcgttgcagc agcggcgact
     2401 gc
//
LOCUS       DAORHD010000795         2400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53804_length_2400_cov_4.492111, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000795 DAORHD010000000
VERSION     DAORHD010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53804_length_2400_cov_4.492111"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="PKO06_15535"
     CDS             <1..1908
                     /locus_tag="PKO06_15535"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71114.1"
                     /translation="RPSMSDTLPENTGMDRSAEVPSTVPEKTAPQKPGVLPEWAQEMS
                     EIYRSGAVSQFILYGNVTDWVQFTPPGSDDPQFVSLQEFLCKGLFAPFEVVLSYDRGK
                     GVRSLKGGELLQQFLKVFDGFHHTSYTSGNLAPNNDPGKVLELGNALPKDPKRALEIV
                     DRFIRNGLYRTKPGPDGKPIPDPMRVALIIDYAQFLIPRTDPGYTSGEIVETLIKILD
                     WASDPMIGKAMVVTCLIAETLADISKQVVENPRSAKIRLDLPNAKDVRDFIGSITADI
                     ADFSSVCDVDRDSLSQKLEGLSRVDLQNLIKRAIKNRQRISMEYLRSVKKELIEKSAG
                     GRMEFVESTKTLDHVAGHVAVKQWLRQDALLMKRGKLKALPMGYLITGRIGTGKTYLV
                     ECFAGECGIPCVELKNFRDKWVGASEGNLEAIFKILHAMGQVIVFVDEADQWAGKRSS
                     EGGDSGLSGRLYAMLAREMADTRNRGRIFWMFATSRPDLLEIDLKRVGRLDVHIPLFS
                     PQTAEERKELFASLAKKNKVKLAEEDFPTFPEDLDIGGNEMEGILIRASRLYELQEDT
                     PDKKPIGSFIREVMQGYKPMAHTERLEYMDLVAVLECTDERFLPAKFAKIPLDQVEQR
                     MAMLKANLGEG"
     gene            2043..>2400
                     /locus_tag="PKO06_15540"
     CDS             2043..>2400
                     /locus_tag="PKO06_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71115.1"
                     /translation="MRLKALALAFLLCCSSASAGPLEGYDDKKLSNPEHKTPKYLFGR
                     VAQNHSLDSVTDKASAEELLKKMLPELREAGMIIEEVKGDKIKVKTEIGWEWVDVIRG
                     AGAPNPGFWWGSEGKPI"
BASE COUNT          551 a          694 c          672 g          483 t
ORIGIN      
        1 aggccctcca tgtctgacac gttgcctgaa aacaccggta tggatcgatc cgcggaagtt
       61 ccgtcaaccg taccagaaaa gactgcgcct cagaagcccg gagtgctgcc tgagtgggca
      121 caggaaatgt ccgaaatcta tcgcagtggc gccgtatcgc agttcattct gtatggcaac
      181 gtcaccgact gggtgcaatt cacgccaccc ggcagcgatg atcctcagtt cgtcagcctt
      241 caggagttcc tctgtaaagg gctttttgca ccattcgagg tagtactctc gtatgatcgc
      301 ggaaaagggg tgcgcagcct caaagggggc gaactgctgc agcagtttct caaagtgttc
      361 gatggctttc atcacaccag ctacaccagc ggcaatctgg ccccgaataa cgacccgggg
      421 aaagtactcg aactgggcaa cgcgcttccc aaagacccca agcgcgcgct cgagatcgtc
      481 gatcgtttca tccgcaacgg tctgtaccgc accaaaccag gtccagacgg caaaccgatc
      541 cccgatccga tgcgagtggc cctgatcatt gattacgccc agtttctgat tccccgcacc
      601 gatccagggt ataccagcgg cgagatcgtg gagacgctga tcaagattct ggattgggct
      661 tcagacccga tgatcggaaa agccatggtc gtgacctgtc tcattgccga aacactcgct
      721 gacatcagca aacaggttgt cgagaatccg cgttcggcga aaattcgcct cgacctgccc
      781 aacgcgaaag acgtgcgcga cttcatcggc tcgatcaccg ccgacatcgc cgatttctcg
      841 agtgtctgcg atgtcgatcg cgactcgctg agccagaagc tcgaagggtt gtcacgcgtc
      901 gatctgcaga acctgatcaa acgcgcgatc aagaaccgtc agcgcatcag catggaatac
      961 ctgcgctcgg tgaaaaaaga actgatcgag aaatcggcgg gcggtcgcat ggaatttgtc
     1021 gaatcgacca agactctcga ccacgtcgcc ggccacgtcg ccgtcaaaca gtggctgcgc
     1081 caagatgcgc tgctgatgaa gcgcggcaag ctcaaagccc tgcccatggg atatctgatc
     1141 accgggcgca tcggcaccgg caagacctat ctcgtcgagt gcttcgccgg tgagtgcggc
     1201 attccctgcg tcgaactcaa aaactttcgc gacaaatggg tcggagcttc tgaaggcaat
     1261 ctggaagcca tcttcaagat tctgcatgcc atggggcagg tgatcgtctt cgtcgatgaa
     1321 gccgaccagt gggcgggaaa acgcagcagt gagggcggcg attccggctt gagcggccgt
     1381 ctctacgcca tgctcgcccg cgagatggcc gacacgcgca accgcggtcg catcttctgg
     1441 atgttcgcca cgtcccggcc cgatctgctg gaaatcgatc tcaagcgcgt cggccgcctc
     1501 gacgtgcaca ttccgctgtt ttcccctcag acggccgaag agcgcaagga gctgttcgcg
     1561 tcactggcaa agaagaacaa ggtcaaactg gccgaggaag acttcccgac cttccccgaa
     1621 gatctcgaca ttggtggcaa cgaaatggaa ggcattctca ttcgcgcgtc gcgtttgtac
     1681 gagttacagg aagacacacc cgacaagaaa cccatcggct cgttcattcg cgaagtcatg
     1741 cagggttaca aacccatggc tcacaccgaa cgactcgaat acatggatct ggttgccgtg
     1801 ctcgaatgca ccgacgagcg atttctgccg gcgaagttcg ccaagattcc actcgatcag
     1861 gtcgaacagc gcatggccat gttgaaagcc aatctcggcg agggctgatc gtcgcctctt
     1921 ctgacatcat ggtgtcggag atcggcgtgt cgttgaagcg cggcttgtgt tctctctcgt
     1981 ctctttgtac catgggtatc tctgttcgac atcaacgatg ttcattcccg gaggaattcg
     2041 tcatgagact caaggcactc gcactggcgt tcttgttgtg ctgcagttcg gcatcagcag
     2101 gccctcttga agggtatgac gacaaaaagc tcagtaaccc ggagcacaag actccaaagt
     2161 atctctttgg cagagtggcc cagaaccact ctctcgactc cgtcaccgac aaggcttcgg
     2221 ctgaagaact tctgaagaag atgctgcctg agctgcgcga ggccggcatg atcatcgaag
     2281 aggtcaaggg tgacaagatc aaggtcaaga ccgagatcgg ctgggagtgg gtagacgtca
     2341 tccgcggtgc cggtgcacca aatccgggtt tctggtgggg atcagagggc aagcctatcg
//
LOCUS       DAORHD010000796         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53857_length_2399_cov_3.395478, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000796 DAORHD010000000
VERSION     DAORHD010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53857_length_2399_cov_3.395478"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="PKO06_15545"
     CDS             <1..1266
                     /locus_tag="PKO06_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV71116.1"
                     /translation="RPLHTLDMGKVSYADDGKTVKFDGRVRMFAVGSYPRREPAQILV
                     DELARGSQSSWILEEIRQPAQGRVQVLVNGDVVAETTGEVVIQPKETLTLAKVEFAKG
                     FQWHGFADRTYRGALSVRWGGQNALDAVVSCDFELVLAGVVPAEISSKAATGALQAQA
                     VAARGEILSKMGLRHCNEGFDTCAEQHCQVYAGDTAVARQIAPVIAPTRGLILQASDG
                     AIVDAVYAANCGGHSEANHLVWTTSADPILAGQWDGPKPNPVLDLSREADVKKFIRTP
                     PKSYCNDKTVEGGDKYRWKTPVLGAKWRKVEEAAGVGRIREIKDFARGVSGRLYKLTI
                     VGESGTKTIMKELNIRKLFGMLRSACFIATWKRDKSGFVDGAVFEGAGWGHGVGMCQT
                     GAQSLAKKGWPFQKILAHYFPGSRLVKTY"
     gene            1433..1603
                     /locus_tag="PKO06_15550"
     CDS             1433..1603
                     /locus_tag="PKO06_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71117.1"
                     /translation="MNFRLMSVVFVLLSLLLLPACITGCGVDDTDSMQTRTNFTIGPG
                     NLEDYSTVASGS"
     gene            1722..>2399
                     /locus_tag="PKO06_15555"
     CDS             1722..>2399
                     /locus_tag="PKO06_15555"
                     /inference="COORDINATES: protein motif:HMM:NF013112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71118.1"
                     /translation="MNRAPGQKAFLLTSFGCQMNLADAQRIRGILQNLGYAEVEKEVE
                     ADLIIFNTCCVRQHAEDRFASRVQALRGLKKHNPRLLIAVGGCIAQKEQEKIFDALPV
                     IDLAFGTNDIERLPELLERLKPGEHRIGPFAGTGVFDGEQADGVVLDRPCMAFVNIIR
                     GCTNFCAYCIVPTVRGPEVSRPLPEIVDFVQNLVAKGVREITLLGQNVNAYGKDLGME
                     EGFATLVE"
BASE COUNT          464 a          702 c          747 g          486 t
ORIGIN      
        1 cggccgctgc atacgctcga tatgggtaaa gtcagctatg ccgacgatgg caaaaccgtg
       61 aagttcgacg gtcgggtgcg aatgttcgcc gtgggttcgt atccgcgtcg cgagccggcc
      121 cagattctgg tcgatgaact ggcccggggc agtcagtcga gctggattct tgaagagatt
      181 cgacagcccg cgcaggggcg cgtacaagtt ctcgtgaacg gcgacgtggt cgccgaaacc
      241 accggcgaag tcgtcattca gccgaaagaa acgctgaccc ttgccaaggt cgagttcgcc
      301 aaagggtttc agtggcatgg gtttgccgat cgcacgtatc gtggcgcttt gagcgtgcgc
      361 tggggtggcc agaacgcact cgatgccgtt gtcagctgtg atttcgaact ggtgctggcc
      421 ggagtggttc ccgccgagat ctcatcgaaa gccgcgacgg gcgcgctgca ggctcaggcg
      481 gtggcggccc gcggcgagat tctctccaag atgggcttgc gacactgtaa cgaaggtttc
      541 gatacctgtg ccgagcaaca ctgccaggtc tacgccggcg atacggcggt ggcgcggcag
      601 atcgcgccgg tgattgcgcc gacccgtggt ctgattctgc aggcctctga cggcgccatc
      661 gtcgatgccg tgtacgccgc gaactgtggt ggtcacagcg aagccaatca cctggtctgg
      721 accacctcag ccgacccgat tctggccggc cagtgggacg ggccgaaacc gaatccggtg
      781 ctcgatctgt cgcgtgaagc cgatgtgaag aagttcattc gcacaccgcc gaaatcctac
      841 tgcaacgaca agaccgttga aggcggcgac aagtatcgct ggaaaacccc ggtgctgggc
      901 gcgaagtggc gcaaggttga agaggccgcc ggtgtcggac gcattcgtga gatcaaagat
      961 tttgcccgtg gagtctcggg tcgattgtac aagttgacaa ttgtcggcga gtcaggaaca
     1021 aagacgatta tgaaagaact gaacattcgc aagctgttcg gtatgctgcg cagtgcctgc
     1081 ttcatcgcca cctggaaacg cgacaaatcc ggtttcgtcg acggcgccgt cttcgagggc
     1141 gcgggatggg gtcacggggt cggcatgtgc cagaccggcg cacagagcct ggccaagaaa
     1201 ggctggccct tccagaagat cctcgcccac tacttccccg gcagtcgcct cgtcaaaacc
     1261 tactgaactg ctcccccgac ctgcaacgtt cgatcgaatt ccgcgagcct cgcaccagtt
     1321 ttccgaggct cgcggtcatt ttccgggtga ttgaaatgat tcactcttcg caagtgggtg
     1381 agaaatgccg acataggatg gtgacccgta acttgcgatg tggaggaacc gtatgaattt
     1441 ccggttgatg tccgtggtgt ttgttctgct ttccctgctg ttgctcccgg cctgcattac
     1501 cggctgcggc gtcgatgaca cggattcgat gcagacccgc accaacttca ccatcgggcc
     1561 cggcaatctc gaagactaca gtacggtggc ctccggaagc tgacgcggct gccacgcgcg
     1621 gccggtctca ccgccggttg cgcgctgagt ccatcgatct gatcgttccc ggctttttgc
     1681 ccggttgagc tctgttcgcc ggcgtgctat tctgccgccc catgaaccgc gctcccgggc
     1741 aaaaagcttt tttactgacc agtttcggct gccagatgaa cctggcagac gcgcagcgca
     1801 tccgcggcat tctgcagaac ctcggctacg ctgaggtcga gaaagaggtc gaagccgatc
     1861 tcatcatctt caacacctgc tgtgtgcgcc agcacgccga agatcgcttt gccagccggg
     1921 tgcaggcgtt gcgtggtttg aaaaagcaca accctcgtct gctgatcgcc gtcggcggct
     1981 gcattgccca gaaagaacaa gagaagatct ttgacgcgct gccggtgatc gatctggcgt
     2041 tcggcaccaa cgacatcgag cggctgcccg agctgctcga acggttgaaa ccgggcgaac
     2101 accgcatcgg tccctttgcc ggaaccggtg tcttcgatgg cgaacaggcc gacggcgtgg
     2161 ttctcgatcg gccgtgcatg gcgttcgtga acatcattcg cgggtgtacc aacttctgcg
     2221 cctactgcat cgttccgacg gtcaggggtc ctgaggtctc acggccgctt cctgagattg
     2281 tggacttcgt gcagaatctc gtggccaagg gcgtgcgcga gatcaccctg ctcgggcaga
     2341 acgtgaacgc ctacggcaaa gacctgggta tggaagaggg attcgcgacc ctcgtcgaa
//
LOCUS       DAORHD010000797         2397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_53931_length_2397_cov_3.062340, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000797 DAORHD010000000
VERSION     DAORHD010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53931_length_2397_cov_3.062340"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..>2397
                     /locus_tag="PKO06_15560"
     CDS             123..>2397
                     /locus_tag="PKO06_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNV71119.1"
                     /translation="MVGTGREQRIQAWLFRVTVLVALLAFAVLSWVVSEKTAAFSREE
                     ALARARETAFHHGLNLRSKILEVLGTARTLRSVFEAALKDRSGIVRDRYDQILIENLR
                     TSDSLVFGIWAVFEPNGFDGRDRALAGQPGQASDGVYIPFAYRKNGTIVMQHDDFSTA
                     VDQDYFTIPRRTKQECVIDPYVDLTAGDVVMSSIAVPIFDGDRVVGVVGVDILLSSFN
                     QEVSQVRIAETGSGFMVGNNGLLIGHPRPDMAGRSVREIGLPEDALQAIAVGKEYDYL
                     KKDASGAHELFYKLVPLRIGLASNPWAFGVTVPMDEVLASSRQITRWTVVIGFAALAL
                     LATALFALRLAVVGPLRRSEESLQRVFDHVDSGILLFSPDRRLIEMNRRVGELLDGSP
                     GMFLEHIVPFGDSDASVAAFWQLILDGTGQRREFDLAAKGDRSRKVLDIFFARVELAG
                     ETRVLATVNDLSERKEAEEALVISDQRIRFQQEELLRLARNRTIASGNLDEALKIITE
                     VTASTLGVARAGIWMYDEPHEYLVCRSLFDTRDRTHLPGDKVALSVIAKYTQTAESER
                     VIPVVHACDDPRTAELRDIYLHPYGIVSLLDASIRSGERMIGVVCHEHVGAPRHWTSD
                     EERFVSSISDLISLAIEADVRRRQEEELRSAKVAAEAANVAKSQFLANMSHEIRTPLN
                     GVIGMMTLLQQESLNPQQREYVDIAKSSATSLMKILTDILDLSKIEAGKLELERAPFE
                     IRRLVRELSQIHGFHARE"
BASE COUNT          473 a          637 c          746 g          541 t
ORIGIN      
        1 gcacgactgt gggttttcgg tgtgtgtcct cggtagcggt accggagatc gtcgtcggcc
       61 gttgaccttc gaaccctcac cgctgtgcga gggcggaaga ggtgtgccaa caggaggagc
      121 gggtggtcgg aactggtcgc gaacaacgaa ttcaggcatg gctcttccgc gtcaccgtac
      181 tggttgcttt gctcgccttt gcggtgctga gctgggttgt ttctgaaaag acagcggcat
      241 tttcgcgcga agaggcgctg gcacgggctc gcgagaccgc gtttcatcat ggtctgaacc
      301 tgcgctccaa gattctcgaa gtgttgggca ctgctcgaac gttgcgttct gtctttgaag
      361 cagcgctcaa agaccggtcg ggcatcgtgc gtgaccggta cgatcagata ctgatcgaga
      421 atctgcgaac gtccgattcg ctggtgttcg ggatctgggc cgtgttcgag ccgaatggct
      481 ttgatggccg tgatcgcgcc ctcgccggac aaccggggca ggccagtgat ggcgtctata
      541 ttccttttgc ctatcggaag aacgggacga tcgtcatgca acacgatgac ttttcgaccg
      601 ccgttgatca ggactatttc accattcccc gccggacgaa acaggaatgc gtgatcgatc
      661 cgtatgtgga tctgacggcc ggcgatgtgg tcatgagcag tatcgccgta ccgatctttg
      721 atggcgatcg ggtggtcggc gtggttgggg tcgatattct tttgtcttca ttcaaccagg
      781 aagtctcaca ggtgcgtatc gccgaaaccg gcagcggatt catggtcggc aacaatggat
      841 tgttgatcgg acacccgcgc cctgacatgg cgggtcgtag cgtgcgtgag attgggctgc
      901 cggaagatgc cctgcaggcc atcgcggttg ggaaagagta tgactatctg aaaaaagatg
      961 ccagtggagc gcatgaactg ttctataaac ttgtaccatt gcgcattggt ctggcatcga
     1021 acccctgggc attcggggtg acggtcccca tggacgaggt gctggcgagt tcgcgccaga
     1081 tcacccggtg gacggtcgtc atcgggttcg cggctctggc tctgctggcc accgcgttgt
     1141 tcgctctgag actggcggtc gtgggcccgt tgcgccgttc ggaagagtcg ttgcagcgag
     1201 tctttgatca tgtcgacagc ggcattctgc tgttcagccc tgatcgccgc ctcatcgaaa
     1261 tgaaccggcg ggtcggcgag ttgcttgatg gttctccagg catgttcctg gaacacattg
     1321 tgccgtttgg cgactcggac gcttctgttg ccgctttctg gcagctgatc ctggatggca
     1381 cgggtcagcg acgcgagttt gacctggcgg cgaaaggtga ccgttccaga aaggtgctcg
     1441 acatcttctt tgctcgtgtc gaactggcag gtgagacacg tgtgctggca accgtgaacg
     1501 atttgtccga acgcaaggaa gccgaagagg ctctggtgat cagtgaccag cgcatcaggt
     1561 ttcaacagga agaattactc aggcttgccc gaaatcgcac catcgcctcg ggaaatctgg
     1621 acgaagcgct gaagatcatt accgaagtca ccgcatcgac gctgggggtc gcgcgcgcgg
     1681 gcatctggat gtacgatgaa ccgcacgagt atctggtgtg tcgctcgctg ttcgatactc
     1741 gtgatcgtac ccatctgcca ggcgacaaag tcgctttgag cgtgattgca aagtatacac
     1801 aaaccgctga atccgaacga gttatcccgg tcgtgcatgc gtgcgacgat ccgcggacgg
     1861 cggaactgag agacatctat ctgcatccgt atgggatcgt ttccctgctc gacgcctcga
     1921 ttcgcagtgg cgagcggatg atcggcgtcg tctgccatga acacgttgga gcaccgcgcc
     1981 attggaccag tgacgaggag cgatttgttt catccatctc cgatctgatc tccctggcca
     2041 tcgaagccga tgtgcgtcgg cgacaggagg aggagttgcg aagtgccaag gtcgccgccg
     2101 aagcggcgaa cgtggccaaa agccagttcc tggccaacat gagtcatgag attcgcaccc
     2161 cgttgaacgg tgtcatcggt atgatgaccc tgttacagca ggagtcactg aacccgcagc
     2221 agcgtgaata cgtggacatt gccaagagca gtgccacttc tctgatgaag attctgactg
     2281 atattctcga cctttccaag atcgaagccg gcaagctgga gctggaacga gcgccgtttg
     2341 aaatccgccg tctcgtcagg gaactctcgc agattcacgg tttccatgcc cgcgaaa
//
LOCUS       DAORHD010000798         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54121_length_2391_cov_4.351884, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000798 DAORHD010000000
VERSION     DAORHD010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54121_length_2391_cov_4.351884"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="PKO06_15565"
     CDS             complement(<1..1755)
                     /locus_tag="PKO06_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HNV71120.1"
                     /translation="MIHRRGSIFAIIIGLLAVMLILALSLSRRFSGQARLVSVADQAT
                     IARYFLESYAGDVLWQIRRGRNDATSKVFPAFRGGTTVPVINFDYRPSSMIAELARDL
                     RITLGPDDPVAYFAEVKTLPYDAQAFRVVKANEEKRGLLGIVCQITYEKRVYTLDQQI
                     PFKVVLPVTPVLRKFVLFLDQLHLEQEMAIGDQDAINIMPIRRNYVRDNSVPLTLFGQ
                     PGAKARERNGKVFLGSDEKKIYLNLVGGLLGGDQGRKEDRTDLWQISPSAFDATAGHE
                     TFQVQELMRDEENKPLLVRGAGIPLVYQGHYARFGLLGYSEEIGDPSSSALGDFKLNN
                     FLKNDPSFVEYQRSSRGLTRASALRLQGDAPFGLLPADIVEKMIDSYRGYERDIYGKV
                     FARFFLLSFFFAPSVGSPIHFSDNPDQELSFDQYGNAEMITFKPKSGRYGNYMSRIVS
                     GGKAPASLGREANIPLNRNSDYDHKLLEARDFPECDGLRLAGPFSRVGEAWFGRGPFD
                     GPGGRPRLKEENITSRICRHFRTQSDFKKAVGLSGPDEKRCWVDGVVYVHEPLTLREG
                     ITCENVRGGVVLVKGPIVL"
     gene            complement(1772..>2391)
                     /locus_tag="PKO06_15570"
     CDS             complement(1772..>2391)
                     /locus_tag="PKO06_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71121.1"
                     /translation="IPTGVLKGRDKPDNDERTPAKLCESFKEPLARSLELNVNSLGIT
                     GIDMVNLMVQVKMTRDMLALAKWTLEKRQDAANSIDQVFVSEPFKRLINKGEYLDLER
                     SGIANFFSGFDPPKDVLKHEIEKFDEAIDDVEEKVERREQEILDLQKLIKGEYEQYHA
                     VLRLVEEAKSETGIDPEISAADADYAPSLLEPLCDLQALNPDAGN"
BASE COUNT          493 a          750 c          656 g          492 t
ORIGIN      
        1 gaggacaatc ggccccttga ccagcaccac accgccgcgc acgttctcgc acgtgattcc
       61 ttcgcgcagg gtgagaggtt cgtgcacata cacgacgccg tcgacccagc agcgcttttc
      121 atcggggccc gacagcccga ctgccttctt gaaatctgac tgggtgcgaa agtgtcggca
      181 gattcggctg gtgatgttct cttctttcag gcgcggtcgc ccccccggcc catcgaatgg
      241 gccacgtccg aaccaggctt cgccgacccg cgaaaagggc ccggccagcc gcaacccgtc
      301 gcattcagga aagtcacgcg cctcgagcag cttgtggtca tagtccgagt tacgattcag
      361 cggaatgttc gcctcgcgcc ccagagaagc cggtgccttg ccaccggaaa cgatgcgtga
      421 catgtaattt ccgtaccggc cactcttcgg tttgaatgtg atcatttcgg cattgccata
      481 ctgatcaaag ctcaactcct gatcggggtt gtcgctgaaa tgaatcggcg agccgaccga
      541 tggggcgaag aaaaacgaca acaggaaaaa ccgggcgaag accttgccgt agatatcgcg
      601 ttcatacccg cgataactgt cgatcatttt ttccacgata tccgccggca gcagaccgaa
      661 cggcgcatct ccctgcagac gcagggcaga ggcacgcgtg agcccgcgcg acgagcgctg
      721 atactcaacg aaagagggat cattcttcag aaagttgttc agtttgaaat cgcccagcgc
      781 cgacgacgac ggatctccga tctcttctga gtagcccagc aggccgaacc tcgcataatg
      841 cccctggtac accagcggaa ttccagcccc gcgcaccagc agcggcttgt tttcctcgtc
      901 acgcatcagt tcctgcactt gaaacgtctc atggccggcc gtcgcatcga acgcgctcgg
      961 tgagatctgc cacaggtcgg tccggtcctc tttgcgcccc tgatcgccac ccagcagacc
     1021 accgaccaga ttcagataga tcttcttctc atcactgccc agaaacacct tgccattgcg
     1081 ctcgcgggcc ttcgcgcccg gctgcccgaa caacgtcagc ggtaccgagt tgtcgcgcac
     1141 gtagttgcgc cgaatcggca tgatgttgat ggcgtcctgg tcgccgatgg ccatttcctg
     1201 ctccagatgc agctggtcaa gaaacagaac gaacttgcgc aacaccggag tgaccggcag
     1261 cacaaccttg aacggaatct gctgatcgag ggtgtaaacc cgcttctcat acgtgatctg
     1321 acagacgatg cccagcagtc cgcgcttctc ttcgttcgcc ttgaccacgc gaaacgcctg
     1381 cgcatcatag ggcagggttt tcacttcggc gaagtaggcc accggatcgt ctggcccaag
     1441 ggttattcgc agatctcgag ccagttcggc aatcatggaa ctcggacgat agtcgaagtt
     1501 gatgaccggc acggtcgtgc cgccgcggaa agccggaaac accttgctgg tggcgtcgtt
     1561 ccgcccacgg cgaatctgcc agagcacatc gccggcataa gattcgagaa agtaccgggc
     1621 aatcgtcgcc tggtcagcaa cactgaccag gcgagcctgc cccgaaaacc gccgcgacag
     1681 cgacagcgcc agaatcagca tcacggccag caggccgatg atgatggcaa agatgctgcc
     1741 gcggcgatga atcatcgttc ccttccccgt gtcagtttcc cgcgtcgggg ttcagggcct
     1801 gcagatcgca gagaggctcg agcagcgagg gcgcgtaatc ggcatcggca gcggagattt
     1861 caggatcaat gcccgtctcg ctcttcgcct cctcgaccag ccgaagcacc gcgtgatact
     1921 gctcatactc gcctttgatc agcttctgca ggtcgagaat ctcctgctcg cgtctctcga
     1981 ccttctcttc gacatcatca atcgcctcgt cgaacttctc gatctcatgc ttgagcacat
     2041 ctttcggcgg gtcgaagcca ctgaagaagt tggcgatacc actgcgctcg agatcgaggt
     2101 attcgccttt gttgatcaga cgtttgaagg gttcagagac gaacacctga tcgatactgt
     2161 tggcggcatc ctgacgtttc tcgagcgtcc atttcgccag tgccagcatg tctcgcgtca
     2221 tcttgacctg gaccatcagg ttgaccatat cgatgccggt gatgccgagc gagttcacat
     2281 tcagttccag gctgcgcgcc agaggttcct tgaagctttc gcacagcttg gccggggttc
     2341 gttcgtcgtt gtcgggttta tcgcgaccct tcaggacacc cgtcggaatc g
//
LOCUS       DAORHD010000799         2387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54272_length_2387_cov_3.787307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000799 DAORHD010000000
VERSION     DAORHD010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54272_length_2387_cov_3.787307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKO06_15575"
     CDS             <1..442
                     /locus_tag="PKO06_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71122.1"
                     /translation="DVFPIKFMGMQETHRVLFGEDILTRITIQPTHLRLRCEQEMKNI
                     LLRLRTLFLRQDGRDLIPIMWSLGTALRETLRLALSLTPDGLLPREEVIVRAAKRFGF
                     DVEPVSLVLAFRERECDLTTEEAEKLYGDLMEAVTVMARAIDKL"
     gene            complement(490..1629)
                     /locus_tag="PKO06_15580"
     CDS             complement(490..1629)
                     /locus_tag="PKO06_15580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71123.1"
                     /translation="METFFATAERAEGAVLARSISRVTMHPVIRALLDHVGGVLAVLN
                     EDRQVLAVNHGFLASLGIPSDSGLLGLRLGEILACPHSREMPGGCGTSRACATCGAVI
                     AMVAVLGGERVSEQTCSLTVNRRGYEEDLFLRVRASPICIDDQTLILIFLQDITYHQK
                     WALLERVFFHDLNNLVSGLTSASELLSLEPLSQETKTLVTTIERTSTRLAQEIALQRL
                     LTQQGTSNYQLDITRVSISAALQELETTFRHHPAAAKKRLSLVPPAADLELLTDKTLL
                     MKVMGNMVLNALEAGYPGQEIRVGADVHESAVTLWVWNPGQIPPAVAGRIFQRNFSTK
                     ADFGRGLGTYSMKLFGEGFLKGKVGFATSEAEGTRFFLELPHQIR"
     gene            complement(1727..>2387)
                     /locus_tag="PKO06_15585"
     CDS             complement(1727..>2387)
                     /locus_tag="PKO06_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71124.1"
                     /translation="GSFSRESASVMVGEVFDNSSRPSNPEFRRRVQEALKDGSVTTYF
                     VLTLLKNSVSSIASIGGASLGGMVGGLLVPGPGAIVGAYLGGKISSTMAKTVVYKLSV
                     DIPIKLCLRKIVKHGRELRQQPRSESSTRGMEKVHRFVTNRVKHEVDREEYKTLDHIL
                     AGMNEQNAEDRIYFVPLLKSFQEMLRFKVIEERDWYASKKFYQLKQRVTDWNLLPHVP
                     F"
BASE COUNT          493 a          734 c          658 g          502 t
ORIGIN      
        1 cgacgttttc cccatcaaat tcatgggcat gcaggaaaca caccgcgttc tcttcggcga
       61 ggacattctg accaggatca cgattcagcc gacgcatctg cgtctgcgct gcgagcagga
      121 gatgaagaac attctgctgc gtctgcgaac cctgtttctg cggcaggatg gtcgcgatct
      181 cattccgatc atgtggtcgc tgggaaccgc gctgcgagaa acgttgcgtc tcgctctttc
      241 gctgacccct gacgggctgc tgccgcgcga agaagtcatt gtccgagcgg ccaagcgatt
      301 cggctttgat gtcgagccgg tttcactggt gttggctttt cgcgagcggg aatgcgacct
      361 gacgaccgaa gaagccgaga agctctacgg cgacctgatg gaagccgtca ccgtgatggc
      421 gcgcgccatc gacaagctct gacttctcca accgtcggcg ggtcttgttg ccctccgcag
      481 gctcgatcgt caacgaatct ggtgcggcag ttccagaaag aaccgggttc cttcagcctc
      541 tgacgtggca aatcccacct tgcccttgag gaagccttcg ccgaacagct tcatggagta
      601 ggtgccgagc cctcgtccga aatcagcctt ggtgctgaag ttccgttgga aaatgcgacc
      661 agcgacggcc ggcggaatct gcccgggatt ccacacccac agcgtgacgg cgctttcgtg
      721 cacgtccgcc ccgacgcgaa tctcctggcc gggatatccg gcttccaggg cgttgagaac
      781 catgtttccc atgaccttca tcaagagagt cttatcggtg agcaactcga gatcggcggc
      841 aggtggcaca agagacaatc gttttttcgc ggcagccggg tgatggcgaa aggttgtctc
      901 aagctcctgc agcgcggccg aaattgagac acgcgtgatg tcgagctgat aattcgaggt
      961 tccctgctgg gtcagcaatc gttgcagggc gatctcctgc gctaaccgtg tcgaggtgcg
     1021 ttcaatagtc gtcaccagcg ttttcgtctc ttgtgacaga ggttcgagac tcagtaactc
     1081 ggacgctgaa gtcaaccccg aaaccaggtt gttcagatca tgaaagaata cccgctcgag
     1141 cagcgcccac ttctgatgat aggtaatatc ctgcagaaaa atgaggatca gcgtctggtc
     1201 atcaatacag atgggactcg cgcgcacacg caggaacaga tcttcctcat acccgcgtcg
     1261 attcaccgtc agactgcacg tctgctcgga cactctctcg cccccgagca cggcgaccat
     1321 cgcgatcacc gccccgcagg tcgcgcaggc acgcgaggtt ccgcacccgc ccggcatctc
     1381 gcgggaatga ggacacgcga ggatttctcc cagacgcaac ccgagcagac cgctgtcgga
     1441 tgggattccg agagaagcca gaaaaccgtg attcacggcc agcacttgtc ggtcttcgtt
     1501 gagtaccgcc aagactccgc ccacatggtc gagcagcgca cgaatcaccg gatgcatggt
     1561 cacccgggag atgcttcgcg ctagaaccgc tccctcggcc cgttcagcgg tggcaaagaa
     1621 tgtttccata cgcgttcctc tctccttcac tgtcttcgtg ttccgacggt ctcgttctgt
     1681 cgggtcaacc agaccttcct cttacgcgcg ctgtctctcc cgatggtcag aacggcacat
     1741 gcgggagcag gttccagtcg gtcacgcgct gcttcagttg atagaacttc ttgctggcgt
     1801 accagtcacg ctcttcgata accttgaagc gaagcatttc ctgaaacgac ttcaacagcg
     1861 gtacaaagta gatgcgatcc tcggcgttct gttcattcat cccggcgaga atatgatcga
     1921 gcgtcttgta ctcttcgcga tcgacctcat gcttcacccg attggtcaca aagcgatgca
     1981 ccttttccat tccgcgggtg gaagactcgc tgcgaggctg ctggcgcagt tcacgcccat
     2041 gtttcacgat cttgcgcagg cagagcttga tcggaatatc gaccgaaagt ttgtagacca
     2101 ccgttttggc cattgtcgat gaaatcttac cgcccagata ggccccgacg atggcgccag
     2161 gccccggcac gagcaggccg ccaaccatgc cgcccagact cgccccgccg atactggcga
     2221 tactcgacac cgaattcttc aacagcgtca ggacgaagta cgtggtgaca ctgccatcct
     2281 tgagcgcttc ctgcacccgg cggcgaaact cgggattgct cggccgactc gagttgtcga
     2341 acacttcacc caccatgacc gaagccgatt cacgggaaaa gctgccc
//
LOCUS       DAORHD010000800         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54588_length_2378_cov_3.083082, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000800 DAORHD010000000
VERSION     DAORHD010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54588_length_2378_cov_3.083082"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PKO06_15590"
     CDS             complement(<1..1535)
                     /locus_tag="PKO06_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71125.1"
                     /translation="MNSVEPAQSTQRRWYACSRGDLHEWGARIIRTLFVVLVFAGFPA
                     WVFHAAFGKIETLEYERRVAALAASHQSLLTRVRTQSNDIEMLRSRIVQLSSIMKDAS
                     PERKKTLVTRFNKAFPGMVDLYFFDHQLQLEPTLSATNIPRRAIEKAMAALVEGNRSK
                     SLSSMQKGILGSVFRSNLVSKSIDLDLEYIMLQPRPEITGIVWGRADSKRLGFIAIFH
                     QKAIPSNLFLSRIIRNAEKSPGRIRIGLYDPKHEPVLIVPESLARDSDIKRGVLMALA
                     QYRQYFLSPDAMVSLTARGDGTYLILRSPLTKRISEKWNGTFAFCALGWAIFIFAQAD
                     KIYNGLGRTIPAKVVILFVLIGGTPSTLLMLTGFYALKDHAHVMREALIRDATERLRL
                     FDERFEDERTAVQQTFAAVVRRAATTFDARKRREILFDLSHLTNLEMVFAVDENGKPL
                     FEHYSQTAVVPDQQKKLLYAIAQQILRTLTGSDKVDGASFASEAVFSAAGSLGGEHLR
                     QVEG"
     gene            complement(1551..>2378)
                     /locus_tag="PKO06_15595"
     CDS             complement(1551..>2378)
                     /locus_tag="PKO06_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71126.1"
                     /translation="GAPELQGRNLVRVPFRIDAEVKVDQTLKLIAMMGMGVALDQFDL
                     LPIEKINQMLQQVPAMHVRTVLQKFFPSLDNFLLNKLNASFFDQLVKQGHVSGQAMLT
                     SIGVGDFLGFLLGFGVGIAESAALAGVTNAAELGVGAAIITMPGVGNVAVGAMLMVVS
                     AKLTQMAFNLGKEVVEWGIWRDRFAKIEGYLQGAWSPGKAQADWLEKQIAYEAARDDF
                     SAVHRLIMHLRVMPLLRRAWFREYVQNLRASIVRQAEAKQSFVAQKYVAIIDVLFLK"
BASE COUNT          561 a          695 c          609 g          513 t
ORIGIN      
        1 ccttctacct gtcgcagatg ctccccgccg agactgccgg cagcagagaa aacggcttca
       61 gaagcaaagg aagcaccgtc caccttgtct gacccggtta aggttcgaag aatctgctgg
      121 gctatcgcat agagcagttt cttctgttga tccggcacaa ctgccgtctg cgagtaatgc
      181 tcaaacaaag gctttccatt ctcatcgacc gcaaaaacca tctcgagatt cgtcagatgt
      241 gacaaatcga acagaatctc gcgccgcttc cgggcatcga acgttgtggc cgcccttcgc
      301 acaactgccg caaaggtttg ctgaacagcc gttctctcat cttcaaagcg ctcatcgaac
      361 agtcggaggc gctcagtggc atcgcgaatc agcgcttctc tcatgacatg cgcatggtct
      421 ttcagggcgt agaagcctgt gagcatcagc agagtggacg gagtaccgcc tatcagaaca
      481 aacaggatga cgacttttgc cggaatcgtt ctgccaaggc cgttgtagat tttgtcggcc
      541 tgagcgaaga tgaagatcgc ccatcccaat gcgcaaaaag caaaagtacc attccatttt
      601 tcggaaatcc tcttggtcag gggcgaccga aggatcagat acgtgccatc tcctctcgcc
      661 gtcagcgata ccatcgcgtc aggtgacaga aaatactgcc gatactgagc caatgccatc
      721 agcacgccgc gtttgatgtc ggaatcgcgt gccagcgatt ccggaacgat cagtaccggc
      781 tcgtgcttcg gatcatacag accaattctt atccttccag gagacttttc tgcgtttctg
      841 atgattcgac tgagaaacag attactcgga atggcttttt gatgaaagat agcgatgaaa
      901 cccagtcgtt tcgaatccgc ccttccccag acgataccgg tgatttcggg ccgcggctgc
      961 aacatgatgt attcaagatc aagatcaatc gacttgctga ccagattcga gcggaagacg
     1021 gagccgagaa tacctttctg catactgctc aggctcttgc tccggttgcc ctcaaccagc
     1081 gccgccattg ctttctcgat tgcccgtcgt ggaatgttcg ttgcactgag cgtcggctca
     1141 agctgcagct ggtggtcgaa gaagtacaga tcgaccatgc cgggaaatgc tttgttgaag
     1201 cgcgtgacaa gggtcttctt ccgttcaggc gaggcgtctt tcatgatcga actgagttgc
     1261 acgatccgac tgcgcagcat ctctatgtcg ttcgattgcg tgcgaacgcg cgttaacaga
     1321 gactggtgag acgccgccag agcggcaacg cgccgttcgt attcgagcgt ttcaatcttt
     1381 ccgaacgccg cgtgaaaaac ccaggcggga aatccggcaa acaccaggac gacgaagagc
     1441 gttctgatga ttcgagcgcc ccattcgtgc aggtcaccgc gagagcatgc gtaccatcgg
     1501 cgctgtgtcg attgagcggg ttcgacagaa ttcacagggc catcgatttc ctatttcaga
     1561 aacaggacat cgatgatggc gacgtatttc tgcgccacaa atgactgttt tgcctctgcc
     1621 tgacgaacga tcgacgcgcg caaattctgc acatattctc gaaaccaggc gcgacgcagc
     1681 aggggcatga cgcgcaggtg catgatcaga cgatgcacgg cgctgaaatc gtcgcgcgcc
     1741 gcttcatacg ctatctgctt ttcgagccaa tctgcctgag ccttgcccgg cgaccatgcc
     1801 ccttgcagat acccttcgat cttggcgaac cgatcacgcc agatccccca ctcaaccact
     1861 tctttgccaa gattgaacgc catctgggtg agtttcgccg agacaaccat cagcatggca
     1921 ccgaccgcga cattaccgac accaggcata gtgatgatcg ctgccccaac gccaagttcc
     1981 gcggcgttgg tcaccccagc cagagcggcc gattcggcaa tgccgacacc aaagccgagc
     2041 agaaatccga gaaagtcgcc gacgccaatc gacgtcagca tggcctgacc tgaaacatgc
     2101 ccctgtttca ccaactggtc gaagaagctt gcgttgagct tgttcagcag gaagttgtca
     2161 agcgaaggaa agaacttctg cagcacggta cgcacatgca tcgccggcac ctgctgcagc
     2221 atctgattga tcttctcgat cggcaggaga tcaaactgat caagggccac tcccatgccc
     2281 atcatcgcga tcaacttcaa cgtctggtcc actttgactt cggcatcgat gcgaaacggc
     2341 acccgcacta gatttcgccc ctgcagttca ggcgcacc
//
LOCUS       DAORHD010000801         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54602_length_2378_cov_2.544124, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000801 DAORHD010000000
VERSION     DAORHD010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54602_length_2378_cov_2.544124"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKO06_15600"
     CDS             complement(<1..492)
                     /locus_tag="PKO06_15600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV71127.1"
                     /translation="MSQIIYSVCAPVFNEQECLPTLVDKIAPVMEALQRPWELLLTDD
                     GSSDRSWDVILELKQRFPFIRGLKFDRNHGQTAAMAAAIRASRGEFIITLDADLQNDP
                     QDIPRLIESLGNHDAAIGWRHDRQDTLVRRLSSRFANWFRNLVTNDGVQDTGCSLKLF
                     RAST"
     gene            complement(489..1418)
                     /locus_tag="PKO06_15605"
     CDS             complement(489..1418)
                     /locus_tag="PKO06_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71128.1"
                     /translation="MKLHVWVVAVGLFLVCGLSSGFGQTAETVLLQGTWGSAPHQLGL
                     RLQAPGELPAAPYMGPGGFRLTPDGALWVSDSVNRCLKRLAKGSPPLLLPVEAERLGD
                     LEVQGHAVSVVTRDPHGIMVFDSESRRRTAWIPFSAKSPGRLLGCGEDRLILEDPADG
                     PWLIENGKVRPHPAKAIEPAGTEARLFGTLYDFDDTSRKIIVAGWKEEESEPELFSLY
                     KSGGRRIVFSRLLNTMGSSPLLTVVTASDPQKLELIGFDEKGNPTRAGFLPILSGPYL
                     ATPWVRTPDGLYIGFSGTPEGFRIVKIAPEPAP"
     gene            complement(1563..>2378)
                     /locus_tag="PKO06_15610"
     CDS             complement(1563..>2378)
                     /locus_tag="PKO06_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HNV71129.1"
                     /translation="YLNFDNLSWWWKLGRAWKAYQDGDFQKAYELAQADTSDPLKARY
                     LMAKSLYGLGKYAEARAMLKGIEKTLEDAAFLQVLDKTAKQYIDEPVVFKFGGFDDLP
                     TYRMKKAKGYPLGLESSVYYDKYVDINTWKWKSDAAYKEFNKIGGIDCSGFVQMVLKE
                     TYGDAGKTWPISGRTSTSGLWSQNFTKEINPGHRPPPPPDIRPGDMILFDYGHNRYGH
                     SAIYRGRDAQGNIHVLQMGDTAQEAIIPAEKLQYYKGTYRMNGMDAVRQKLTA"
BASE COUNT          490 a          667 c          724 g          497 t
ORIGIN      
        1 cgttgacgcg cgaaacagct tgagcgaaca gccggtatcc tgaactccgt cgttggtcac
       61 caggttgcga aaccagttgg cgaagcgact cgacaggcga cggaccaggg tgtcctggcg
      121 gtcgtggcgc cagccgatgg cggcgtcgtg gttgccgaga ctctcgatca aacgcggaat
      181 atcctgcggg tcattctgca gatcggcatc gagcgtgatg atgaactcgc cgcgggatgc
      241 gcggatggcg gctgccatcg ccgctgtctg gccgtggttg cggtcaaact tcaacccgcg
      301 aatgaaggga aaacgctgct tcagttccag gataacgtcc cagctgcgat cgctcgagcc
      361 atcatcggtg agcagaagtt cccacgggcg ctgcagtgct tccatgaccg gggcaatttt
      421 gtcgaccagc gttggcaggc attcctgctc gttgaaaacc ggggcacaga ccgaatagat
      481 gatctggctc acggggctgg ttccggggcg attttgacga tgcgaaaccc ctcgggggtt
      541 cctgaaaagc cgatgtagag gccgtccggc gtgcgcaccc agggggtggc aagatagggg
      601 cctgacagga tcggcagaaa ccccgcgcgg gtcggattgc ctttctcgtc gaaaccgatt
      661 aactcgagct tctgtgggtc tgaagccgtg acgaccgtta acaggggcga gcttcccatg
      721 gtgttcagca ggcggctgaa aacaatgcga cgtccacccg acttgtacaa actgaaaagt
      781 tcgggctcgg attcttcttc cttccagccg gcgacgatga ttttgcggct ggtatcgtcg
      841 aagtcataga gggtgccgaa aagtcgcgcc tcagtaccag ccggttcgat ggctttggca
      901 gggtgggggc ggactttgcc gttttcgatc agccaggggc cgtcggccgg gtcttccagg
      961 atcaggcggt cttcgccaca accgagcagt cgacccggag atttggcgga aaaaggaatc
     1021 caggcggtgc ggcgtcgcga ttcactatca aaaaccatga tgccatgcgg atcgcgggtg
     1081 acaacagaga cggcgtgacc ttgtacctcc aggtcgccga gccgttcggc ctcaaccgga
     1141 agcagcagtg ggggagaacc tttagccaac cgcttgagac accgattcac cgaatcgctg
     1201 acccagagcg cgccatcggg cgtcagacga aaaccacccg gacccatgta gggcgcggcc
     1261 gggagctcac cgggtgcctg cagacgcaga cccagttgat gaggcgctga accccatgtg
     1321 ccttgaagca gcactgtttc ggctgtttgt ccgaagccgg aagacaaacc gcagacgagg
     1381 aacaacccaa ccgccacgac ccatacatga agtttcatgc tgattgcctg tccgagatca
     1441 gattttccgc aagccacaag tcgccagagc atagcagatt ttcatctgtc cgaaaagggg
     1501 acggacccca gccgcttcag cagcggaatc ggggcccgtt gagtgtgagc ggaaagttct
     1561 gctcaggcgg tcagtttctg acgcacggca tccattccgt tcatgcggta ggttcccttg
     1621 taatactgca gtttttccgc cggaatgatg gcttcctgtg ccgtgtcacc catctgcagc
     1681 acgtgaatgt tgccctgggc gtcgcgaccg cgatagatcg ctgaatgccc gtagcggttg
     1741 tgcccgtagt cgaagagaat catgtcaccc gggcgaatgt ccgggggcgg cggggggcgg
     1801 tgcccgggat tgatctcttt ggtgaaattc tgcgaccaga gaccgctggt cgaggttctg
     1861 cccgagatcg gccaggtctt gccggcatcg ccgtaggttt ccttgagtac catctgcacg
     1921 aaaccgctgc aatcgatgcc accaatcttg ttgaattcct tatacgcggc atccgacttc
     1981 catttccagg tattgatgtc aacatacttg tcgtaataca cggaactttc gagaccgagc
     2041 ggatacccct tggctttttt catgcgatag gtcggcaggt cgtcaaagcc gccgaacttg
     2101 aagacgacag gttcgtcgat atactgcttg gcggtcttgt cgagaacctg cagaaacgca
     2161 gcatcttcga gggtcttctc gattcctttc agcatggctc gggcttcagc gtatttgccg
     2221 agaccgtaca ggctcttggc catgagatag cgcgctttca gcgggtctga agtgtcggcc
     2281 tgcgccagtt cataggcttt ctgaaagtcg ccgtcctgat aggctttcca ggcgcgaccc
     2341 agtttccacc accaggaaag attgtcgaag ttcagata
//
LOCUS       DAORHD010000802         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54627_length_2377_cov_3.086133, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000802 DAORHD010000000
VERSION     DAORHD010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54627_length_2377_cov_3.086133"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="rplM"
                     /locus_tag="PKO06_15615"
     CDS             complement(<1..380)
                     /gene="rplM"
                     /locus_tag="PKO06_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNV71130.1"
                     /translation="MKTFMPKAADIKRDWLLVDAKGKVLGRLATQIATLLRGKHKTTF
                     APHADTGDFVVVINAGEIVLTGAKHTDKTHHWYTGYPGGLKTVTYGEMLQEDPARMLT
                     LAVKRMLPNTHSRHNLLTRLKIYPG"
     gene            complement(396..1118)
                     /gene="truA"
                     /locus_tag="PKO06_15620"
     CDS             complement(396..1118)
                     /gene="truA"
                     /locus_tag="PKO06_15620"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV71131.1"
                     /translation="MVVTYDGSAFFGFQFQPGVPTIQGELESVAGRVLGEKVRCIGSG
                     RTDTGVHAMAQVVGVKTSCPIPVEKLYLAMNRSLPGSIRVRSVDEADPGFHPRFSATG
                     KHYRYLIRPVTEPSPFLERYVWQVIESLEVERMQQAAAVFVGTHDFSSFARSPERFED
                     PRRTILSSAVRREGDVIVYDVVGTGFLHNMVRNMARALVLAGSGKLQIEDVIELLRRQ
                     DRRRLGPPAPPAGLYLMEVRYS"
     gene            complement(1164..1925)
                     /locus_tag="PKO06_15625"
     CDS             complement(1164..1925)
                     /locus_tag="PKO06_15625"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNV71132.1"
                     /translation="MKPLFTFEPGTGFWYRVHPMAKGVTAAVAVLTVSLQGGGLVVAG
                     CLCATILLLGAAGGLRAGEALTGLRSLTLLFLVMAGFQLLQPEGTWQHALESCFRLAG
                     VFLVSGFFVMSASQSELMFFWEGCFRPLGLIGLPSRELALVMVIAVRFLPVFFAEIER
                     IRMAQSARGANFAGNGWTGAAKRYLPLLIPVLELSIRRAEELAVAMEARGYCLHSPRS
                     RYRVYHFSGLDVTILTIILAGSFVLLGGTRILPVR"
     gene            complement(1922..>2377)
                     /locus_tag="PKO06_15630"
     CDS             complement(1922..>2377)
                     /locus_tag="PKO06_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71133.1"
                     /translation="RAWGIDAERFWERHPSRLSGGEKRRVALAACSVFQPPLLLFDEP
                     TAGLDFPGRESLRQRLVQLAQEHLVIVVTHDPAELLADAGSVLVLAEERALWYKSTSE
                     FLAAAFADRDVYPLPDWYRQAVAPRRTTDRLLPVLTAQAVHQWLKDEVA"
BASE COUNT          545 a          736 c          668 g          428 t
ORIGIN      
        1 ccaggataga tcttcagacg ggtcagcagg ttgtggcggc tgtgggtatt gggaagcatg
       61 cgcttcacag ccagggtcaa catgcgggcc gggtcttcct gaagcatttc accataggtg
      121 acggtcttga ggccaccggg atatccagtg taccaatgat gggttttgtc agtgtgcttg
      181 gcgccggtga gaacgatttc tccagcgttg atgacgacga cgaagtcgcc ggtatcggca
      241 tgaggcgcga aggttgtctt gtgtttaccg cggagcaggg tggcaatctg ggtggccagt
      301 cgccccaaaa ccttcccctt ggcgtccacc aacaaccaat cccgcttaat gtcagcggct
      361 ttgggcatga acgtcttcac gtactttcct cctgattatg aataccgtac ctccatcaga
      421 tacaggccgg ccgggggggc tgggggtccc aggcgtcgcc gatcctgacg gcggagcagt
      481 tcgatcacat cttctatctg gagctttccg ctgccggcaa gaaccaacgc tctcgccatg
      541 ttgcggacca tgttgtgcag aaacccggtg ccgaccacat catacacgat gacgtcgcct
      601 tcgcgccgca ctgcagatga cagaatcgtg cgccgggggt cttcaaaccg ttctggtgac
      661 cgggcaaacg aggaaaaatc atgcgtgccg acaaagacgg cggcggcctg ctgcatgcgc
      721 tcaacctcga gagattcgat cacctgccac acataacgtt cgagaaacgg gctcggttcg
      781 gtcaccggcc ggatcagata acggtagtgt ttgccggtcg cggaaaacct cgggtgaaac
      841 ccagggtctg cttcatcgac cgaccgcacc cggatggacc cgggcagaga acggttcatg
      901 gccagataga gcttttcaac cggaatcgga caactggttt tcacaccaac cacctgggcc
      961 atggcatgca ccccggtgtc tgtccttcca gacccgatac agcgcacctt ttcgccgaga
     1021 actctgccgg cgacactctc gagttcgccc tggatcgtgg gaaccccagg ctggaactga
     1081 aagccgaaaa aggcactacc gtcgtaggta acgaccattt taagatttct ctgacgcaaa
     1141 gtcaagtgtt tcccctcatt tcatcagcgt acaggtaaaa ttcttgtacc acccagcaga
     1201 acgaaggaac ctgccagaat gatggtcaag atcgtgacat cgagccccga gaaatggtac
     1261 acccgatagc gcgatcgtgg cgagtggagg cagtaccctc gcgcttccat cgccaccgcg
     1321 agttcctcag ctcgacgaat actcaattcg agcaccggaa tcaacagggg cagataccgc
     1381 ttggcggctc ccgtccagcc attgccagcg aagttcgcac ctcgcgccga ctgcgccatg
     1441 cgtatgcgct cgatctccgc aaagaagacc ggcaggaagc gtaccgcgat caccatgacc
     1501 agagccagct cgcgactggg caacccgatc agccccagag ggcggaaaca cccttcccag
     1561 aaaaacatca gctcgctctg tgacgcgctc atcacaaaga aacccgagac cagaaagaca
     1621 cccgcaagcc gaaaacagct ttccagagca tgttgccagg ttccctcggg ttgcaggagt
     1681 tggaaaccgg ccatgaccag aaacaagagc gtcagactgc gcaagccggt gagagcctcg
     1741 cccgctcgca gaccgccagc agcgcccagc agcagaattg tcgcgcagag gcaaccggca
     1801 acgacgagtc ccccgccctg cagtgacacg gtcagcacgg caacggcggc cgtcacgccc
     1861 ttcgccatgg gatgcacccg ataccagaag cctgtacccg gctcaaaggt aaagagcggt
     1921 ttcacgcaac ttcgtccttc aaccactgat gcacggcctg tgccgtaaga accggcagaa
     1981 gccggtcggt cgttcgccga ggcgcaacgg cctgccgata ccagtcggga agcggataca
     2041 cgtctcgatc tgcgaaagcc gcggccagaa attcactggt gcttttgtac cagagggcac
     2101 gttcctcggc caggaccaac acgcttccgg catccgcaag caactcggcg ggatcatgcg
     2161 tgaccacgat caccaagtgt tcctgagcca attgtaccag tcgctgacgt agagattccc
     2221 ttcccggaaa gtccaggccg gccgttggct cgtcaaagag cagcaggggt ggctgaaaca
     2281 ccgaacacgc tgccagagct acgcgacgtt tttctccccc agagagacga ctggggtgac
     2341 gttcccagaa ccgctcggca tcaatgcccc aggctcg
//
LOCUS       DAORHD010000803         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54654_length_2376_cov_4.081430, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000803 DAORHD010000000
VERSION     DAORHD010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54654_length_2376_cov_4.081430"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKO06_15635"
     CDS             <1..1140
                     /locus_tag="PKO06_15635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNV71134.1"
                     /translation="GDWMYLAGVGATPQGDLPFLRRFRLSDRTTQEMFRSATGTYELF
                     VGFSDEVAGGMVTRYETPTISPNYRLRSLATPSSSIATLTAFPDPAPELNGIRKELLQ
                     YKRADGIPLSGTLYYPVDYASGTRVPVIIWAYPREYVSPETAGQVRAVPNRFARLEGM
                     SILLLLLEGYAVLFNAEIPVVGKPLEANNTYVEQITSAAQAAVDTLVEMGIADRHRIG
                     IGGHSYGAFMVANLLAHTDLFAAGVARSGAYNRTLTPFGFQAERRTYWQATEIYTRLS
                     PFTYAHQINEPLLLIHGELDENTGTFPLQSERLYAAIKGNGGTSRLVLLPGEGHAYRA
                     RESILHVLAETLSWCDTHIRNRPLPPAKTASESVPLESPRPVSGR"
     gene            complement(1230..2162)
                     /locus_tag="PKO06_15640"
     CDS             complement(1230..2162)
                     /locus_tag="PKO06_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71135.1"
                     /translation="MDVSAAWVTALAGRALVAGTPLLLGTLGETLTERAGILNLGIEG
                     LMAVGAVTGFGVAFSTGNPWLGASAAVGIGMLLGLLHAVVTVTLRANQVVAGLALTMF
                     GLGLSSLLGKPYVGKPLPQPIGEWHLPILSDLPILGESLFTRDPLFYLGIGLSLLLWF
                     VLFRTRWGVIVRACGESPLAAATSGVDVQWVRYVCLMSGSGLCGLAGAYLSLVYNPSW
                     VEGMTAGRGWIVVALTIFAFWNPLRAIFGAYAFGGIYVVQYSLQSWNVSPNLLMTLPY
                     VLTLVALVLISSKASRHLAAPASLSKPFFPGEKN"
BASE COUNT          507 a          709 c          726 g          434 t
ORIGIN      
        1 ggagactgga tgtatcttgc cggcgtcgga gccacgcccc agggagatct gccgtttctg
       61 cggcgtttcc gtctgtctga ccgcacgact caggagatgt ttcgatcggc caccggcacc
      121 tacgagttgt tcgtcgggtt ttctgacgag gtcgcgggag ggatggtcac tcggtacgag
      181 acgccgacga tctcacccaa ctatcgcctt cgctctctgg caacgccgag cagctcgatt
      241 gccacattga ctgcctttcc cgacccggca cctgaactca acggtattcg aaaagaactg
      301 cttcagtata agcgtgctga cggaattccg ctctctggaa cgttgtacta tcccgttgac
      361 tatgcgtcgg gcacacgtgt accggtgatc atctgggcct acccacgtga atacgtgagc
      421 cccgaaacgg cggggcaggt gcgggcggtg ccgaatcggt tcgctcgcct cgagggcatg
      481 tcgattctgc tgttattgct ggaaggctac gccgtgctgt tcaatgccga gattcccgtg
      541 gtcggcaaac cgctcgaggc gaacaacacc tacgttgagc agattacgtc ggcggctcag
      601 gccgcggttg ataccctggt cgagatgggc atcgctgatc gccatcggat cgggatcggc
      661 ggacacagtt acggggcgtt catggttgcc aatctgctgg cgcataccga tctgtttgcc
      721 gccggcgtgg cacgaagtgg ggcgtacaat cgcacgttga ccccattcgg ttttcaggcc
      781 gaacggcgca cctactggca ggccaccgag atctataccc gtctctcgcc ctttacctat
      841 gcgcatcaga tcaatgaacc gctgttgctg attcacggcg aactcgacga aaacaccggc
      901 acctttccgt tgcagagcga acgtttgtat gcggcgatca aggggaatgg cggcacgtca
      961 cgactggtac tcttgcccgg agagggacac gcctatcggg cgcgcgagtc gatactgcat
     1021 gtgctggccg agactctgag ctggtgtgac acacatatca ggaatcgtcc gcttccgccc
     1081 gcaaaaaccg catcagagtc cgtgccgctc gaatccccgc ggcccgtcag cggtcgctga
     1141 tggtgcaccc gctctggcgg gtgttctgtg gtgctgtgat gcgtgatgtg tcgaagatat
     1201 gacactgtat caactcggac aaatggcgtt cagttcttct cgccggggaa aaacggtttg
     1261 ctcagtgagg ccggggccgc gagatggcgg ctggccttcg atgagatgag cacgagcgca
     1321 accagggtca gcacgtaggg gagcgtcatc agcagattgg gcgagacatt ccagctttgc
     1381 agggaatact ggacgacgta gatgccgccg aaggcatacg cgccgaagat cgcccgcagc
     1441 ggattccaga acgcgaagat ggtcagggcc acgacgatcc agccgcgccc cgcggtcatg
     1501 ccttccaccc aggaaggatt gtagaccagc gacagatatg caccagccag cccgcagagc
     1561 ccgctgccgc tcatcaggca cacgtagcgc acccactgaa catcgacccc actcgtcgcg
     1621 gcggccaatg gagactcgcc acaggcccgc acgatcacac cccagcgggt gcgaaacagc
     1681 acgaaccaca gcagcagact gagcccgatg cccagataga agagcgggtc ccgcgtgaac
     1741 agtgattctc cgagaatcgg caggtcggaa aggatcggaa gatgccactc accaatcggt
     1801 tgtggcaggg gttttccgac gtacggtttt ccgagcagac tgctgagtcc gaggccgaac
     1861 atggtcaggg ccagaccggc gacgacctga ttggcacgca gagtcacggt gaccaccgcg
     1921 tgcaacagac cgagcagcat gccgatacca acggctgccg atgcgccgag ccacggattg
     1981 ccggtcgaga aggcgacccc aaaaccggtg acggcaccga cggccatcaa tccttcaatg
     2041 ccgagattga ggatgccggc acgctccgtc agcgtttctc cgagcgttcc cagcagaagg
     2101 ggagtccctg ccacgagggc acgacccgca agagcggtca cccaggctgc agaaacgtcc
     2161 atgttgttct ctccttgaat gctgaaatga tgctgattcg tggtctcaga gacctcgatg
     2221 gagtcgcggt cgatactcga gcagcacgtc gccgataatg aggccgatca gaatcagggc
     2281 gttgaagaga tgaacaacgg caaacgggag ttgcagcgag gtctggaaca catcgccgcc
     2341 gaccaggatg ccgccgaaga atagcgccga aacgat
//
LOCUS       DAORHD010000804         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54806_length_2372_cov_3.214933, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000804 DAORHD010000000
VERSION     DAORHD010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54806_length_2372_cov_3.214933"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="PKO06_15645"
     CDS             <1..558
                     /locus_tag="PKO06_15645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV71136.1"
                     /translation="HKAVLVANASSSTAIRSPRPSGPPWQIVSAGAHYEWIDIPWLER
                     LAAHPQVRLHIAGPGRGTGFRNLLSLSGVTYHGVLSQSQLNALFQRCHTGVIPFVDSE
                     LTRAVDPIKAYEYAAAGLRVWASPVPGLDTHPCVTDIISDQVSFMQAIDRLPLPISQR
                     SVPTWADRAREVLERLTVTRPGESR"
     gene            555..1529
                     /locus_tag="PKO06_15650"
     CDS             555..1529
                     /locus_tag="PKO06_15650"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNV71137.1"
                     /translation="MNALLVGFFGEGNLGDEAILQGVLQTLPSSRRLLVTAGRHRPVS
                     GPLYLPRQGLLAWHQFIQSLPTCRHLWFAGGILQDWSFAGTTFFGLRVLAAHHLGRTP
                     GFWGAGIGPLHRRASRRFVKRVLRYPDPVWLRDQQSVDLYHTLTGRQAHLGADWSWAL
                     EPTVTPRASAVAGRLGLNLRPWFNPDCEEHVRLLLGNRLQTSSLCGISARPEDSRLIR
                     QMVSGIEIFSFESFSELLSIATHLREGWAMRYHVVLAMLRANLPTIPLPYDEKVRALC
                     EEAGIPLPADPGQAPFVACRPATDFPRRLQQRFQSMRAAFLAELEKDS"
     gene            1526..>2372
                     /gene="lpxK"
                     /locus_tag="PKO06_15655"
     CDS             1526..>2372
                     /gene="lpxK"
                     /locus_tag="PKO06_15655"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNV71138.1"
                     /translation="MKTLLFRGACGLYKSLWHLEYQFFRSLPFLKYCPRLPVISVGNL
                     TVGGTGKTPLLLALLSELVPRYRVAVLTRGYRSPWERSFYLIQGSGPHPSELTDETVL
                     VHQRFPEVPILLGKNRYHSARRAERWLKPDLLLVDDGFQYRRLQQDFRLLLWDATDAP
                     EQARLLPWGRLREPLERLRDASVIVLTRTELVSSSRLAETRQILSELAPGVPLMNTKI
                     QAEPLKLWNGHPASPAVGEKRLLPFAAIARPEHFATHLHSLGYQQVEPIWFRDHHRFS
                     DSDLEH"
BASE COUNT          418 a          772 c          638 g          544 t
ORIGIN      
        1 cataaggctg ttctcgtggc caatgcctca tcatcaaccg ccatccgttc tcctcgacct
       61 tcaggtcctc catggcagat tgtctcagcc ggagctcact atgaatggat cgacatcccg
      121 tggctcgaac gtcttgccgc tcacccgcag gtgcgtctgc acattgccgg gccgggaaga
      181 ggcacaggat ttcgcaacct gctaagcctt tcgggggtta catatcatgg agtcctatct
      241 cagtcgcaac tcaacgcgct tttccagcgt tgtcacaccg gtgtcattcc ctttgtcgat
      301 tccgagctca cccgcgctgt cgatccgatc aaggcttatg aatatgccgc ggctggcctt
      361 cgggtctggg cttcacccgt tcccggactg gacactcacc cctgtgtcac cgatatcatc
      421 tctgatcagg tcagtttcat gcaggccatt gatcgactcc cacttccgat atcacaacgg
      481 tccgttccta cctgggccga tcgagctcgc gaggtgctgg agcgactgac cgtcacgcga
      541 ccgggagaat cgcgatgaat gcgcttctgg tcggcttttt cggtgaaggg aatctcggag
      601 acgaggcgat tcttcaggga gttttgcaga ccctgccgag ttcacggcgc ctgctggtga
      661 ccgccggtcg acaccgaccg gtctcagggc ctctctatct gcctcgccag ggtttgctgg
      721 cctggcacca gtttatccag tcgctcccca cctgccgcca tctctggttt gccggcggca
      781 ttctgcaaga ctggagtttc gccggcacca cattcttcgg cttacgtgta ctggcggccc
      841 atcaccttgg ccgcactcca ggcttctggg gagcaggcat cggtccgctg caccggcgag
      901 cctcgcgccg gttcgtcaaa cgcgtattac ggtatcctga ccctgtctgg ttgcgcgacc
      961 agcaatccgt cgacttgtac cacacactca ccggacgtca ggcgcacctt ggagccgact
     1021 ggtcctgggc tctcgagccc acggtgacgc ctcgggcatc cgccgttgca ggtcgccttg
     1081 gcctgaacct tcgcccgtgg ttcaatcctg actgcgaaga acatgtccgt ctcttgctgg
     1141 ggaacaggct ccagacatca tctctctgcg gtatctcggc ccgccccgag gattcccggc
     1201 tcatcagaca gatggtttca ggcatcgaga tcttctcgtt cgaaagtttc tcggaactgc
     1261 tatcaatcgc gacgcatctg cgcgagggct gggccatgcg ctatcacgtg gtactggcga
     1321 tgctgcgcgc caacctgccg accatcccgc tgccgtatga cgaaaaagtt cgcgccctct
     1381 gcgaagaagc gggcattcct ctccccgccg atccagggca ggcccctttt gtcgcgtgtc
     1441 gtcccgcgac cgactttccc cgccgactgc aacaacgatt tcagagcatg cgcgcggctt
     1501 ttcttgccga actggagaaa gactcatgaa aaccctgctg ttccggggag cgtgcggttt
     1561 gtacaaatca ctctggcatc tcgaatacca gtttttccgt tcgctgccgt ttctgaaata
     1621 ctgccctcgt cttcccgtta tctccgtcgg caatctgacg gttgggggca ccggaaaaac
     1681 gccccttctg ctggccctgc tcagcgaact ggttccgagg tatcgcgtcg cggtgctgac
     1741 ccgcggctat cgctccccct gggaacgttc tttctatttg attcagggaa gcggaccaca
     1801 tccgtccgag ttaacagacg aaacagtatt ggttcatcag cgctttcccg aggtgccgat
     1861 tctgctcggc aaaaaccgat atcactcggc ccgtcgagcg gaacgatggc tcaagcccga
     1921 cctgctcctc gtcgatgacg ggtttcaata tcgtcggctg cagcaggact tccgattgct
     1981 cctgtgggat gcgaccgacg cgcccgaaca ggcccgcctg ctcccctggg gtcgtttgcg
     2041 cgaacccctc gagcgactgc gcgatgcctc cgtcatcgtt ttgactcgaa ccgagttggt
     2101 gagctcatcg cgacttgccg aaactcgtca gatattgtcc gagttggcac cgggcgttcc
     2161 gttgatgaac acaaagatac aggcagaacc tctcaaactc tggaacgggc atccagcctc
     2221 tcccgctgtc ggtgagaagc ggctgcttcc ttttgccgcg atcgcgcgcc cggaacactt
     2281 tgccacacat ctgcattcac tcggctacca gcaggtggag ccaatctggt ttcgcgacca
     2341 tcatcgtttc agtgatagcg acctcgagca tc
//
LOCUS       DAORHD010000805         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54811_length_2372_cov_2.886923, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000805 DAORHD010000000
VERSION     DAORHD010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54811_length_2372_cov_2.886923"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2152
                     /locus_tag="PKO06_15660"
     CDS             <1..2152
                     /locus_tag="PKO06_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV71139.1"
                     /translation="AQQIYSNAQAHGAMPAVAQTTDTNSFETRIGTVPAHAKATVDLT
                     YSEILTYKNGRIFYTLPLNVQKVQNKSLDLVTVQITIKDQKDIIEVVSPSHGAKASKI
                     DDHTWKMVYEKNSHLPDSDFSLEYEVKAQQMGFNFLSTRPAAGEDGYFMMLMAPQEII
                     SAKDVAARDIVFVMDVSGSMDGYKLEQTKSAFNFFVSQLNGDDRFNVTVFSDNVKRFS
                     EEMVSATQANREKAREFIANVQAMGGTNIHEAITDARRLFYGNRGNQTKAIVFLTDGQ
                     PTVGMTDVDQIAGSIKAGNDLGLRLFVLGVGEDVSTRLLDKLSLENRGETIYVRQNEN
                     IEGKLTAFYETISKPLLVDVTVDWNGKTVSEIYPKTLPNLYKGSQLVLTGRYSTSGAS
                     RLMVRGDLNGKKMEFPLEVNFADKGSSNGFVARVWAKSKADALIQEMNAYGENSGMKA
                     EVIRLSKTFQFTTPFTSFISVAPQQVVAAPSAHNQFRTSWNQPQVTPYSAPAPAAQPY
                     VPPAPVVTGPPQTVTVVRKTEAKEVKMWGQYSFFPLAPLLVPNFRKAREQARWKACYA
                     NQRVLLGAIEMYNMDHSVQLSAYNPSVEPVLVNGQYLKTPLTKPETGCGYYGVSLNTA
                     SGAIACAMHGGVEADNMVTIDPKTQQVCPVVVQYQDVKDWKVDLWNFLAPLVEILINV
                     PIALLGLWFTWVCFRTFVLSPLMFILGFTSPSNS"
BASE COUNT          520 a          692 c          651 g          509 t
ORIGIN      
        1 tgcgcagcag atctattcga atgcccaggc gcacggagcc atgcccgccg tcgctcagac
       61 caccgacacg aactcgttcg aaacccgtat cggcactgtt ccggctcatg ccaaggcaac
      121 ggtcgacctg acctacagcg agattctcac ctacaaaaac ggtcgtatct tctacaccct
      181 gccgttgaat gtccagaaag ttcagaacaa gagtctcgat ctggtcacgg ttcagattac
      241 catcaaagat cagaaggaca tcatcgaagt cgtatctccc agccacggtg ccaaagcgag
      301 caaaattgat gatcatacct ggaagatggt ctatgaaaag aactcacacc ttcccgactc
      361 ggacttttca ctcgagtatg aagtgaaggc gcagcagatg gggttcaatt tcctgtcgac
      421 ccggccagcc gctggcgaag acgggtattt catgatgctc atggccccgc aggaaatcat
      481 cagcgcgaaa gatgtcgctg cccgcgatat cgtgttcgtc atggatgtgt caggtagtat
      541 ggacgggtac aaactggaac agaccaagtc agccttcaac tttttcgtct ctcagctcaa
      601 tggtgacgat cggttcaatg tcaccgtgtt ttctgacaac gtgaagcggt tcagcgaaga
      661 gatggtgtcg gcgacgcagg ccaatcgcga gaaggctcgc gagttcatcg ccaacgtgca
      721 ggccatgggc ggcaccaaca ttcacgaagc cattaccgat gcgcgccgtc tgttctacgg
      781 caatcgtggt aatcagacca aggcgatcgt gttcctgacc gacgggcaac cgactgtcgg
      841 catgaccgat gtcgatcaga ttgctggcag catcaaagcc ggcaatgacc tgggcctgcg
      901 cctgtttgtc ctgggtgtgg gcgaagatgt cagcacccgc ctgctcgaca agctgtcgct
      961 cgaaaatcgc ggtgaaacca tctatgtgcg ccagaacgaa aacatcgagg gcaagctgac
     1021 ggcgttctac gaaaccattt ccaagcccct gctggtggat gtcaccgtcg actggaacgg
     1081 caaaaccgtc tccgagatct acccgaagac cctgcccaat ctgtacaaag gctctcagct
     1141 ggttcttacc ggccgttact cgacctcggg agccagtcgt ctgatggttc gcggtgatct
     1201 gaacggtaag aagatggaat tcccgctcga agtcaacttt gccgacaagg gctcatcgaa
     1261 tggatttgtg gctcgcgtct gggccaagtc gaaagccgat gcgctgattc aggaaatgaa
     1321 tgcgtatggc gaaaattctg gaatgaaggc tgaagtcatt cgtctgagca agaccttcca
     1381 gttcacgacc ccgtttacct cgttcatctc cgtcgctccg cagcaggtgg tggctgcacc
     1441 ttcggcacac aaccagttcc gcacttcctg gaaccagcct caagttaccc cgtacagtgc
     1501 gcccgcaccc gcggctcagc cgtatgttcc ccctgctcca gtcgtgaccg gcccgccgca
     1561 gaccgtgacg gtggttcgca agaccgaagc caaggaagtg aaaatgtggg ggcagtacag
     1621 tttcttcccg ctcgctcctt tgctggtgcc caacttccgc aaagctcgcg aacaggctcg
     1681 ttggaaggcc tgctatgcca accagcgcgt gctgctgggt gccatcgaga tgtacaacat
     1741 ggatcattct gtgcagttgt cagcctacaa cccttcggtc gaaccggtgc tggtcaacgg
     1801 acagtatctg aagacgccgc tcacgaagcc tgaaacgggc tgtggctact acggcgtgag
     1861 cctcaataca gcaagtggag ccatcgcctg tgccatgcac ggtggtgtcg aagcggacaa
     1921 catggtgacc atcgacccga agactcagca ggtgtgcccg gtcgtcgtgc agtatcagga
     1981 tgtcaaagac tggaaggtcg atctctggaa cttcctggcc ccgctggtcg agattctgat
     2041 caatgtgccg atcgccctgc tgggcctgtg gtttacgtgg gtgtgtttcc gaaccttcgt
     2101 tctgtcgccg ttgatgttca tcctgggctt cacttcgccg agcaactcgt aagcaatcac
     2161 tccgccgggt cggtcgttga gactggcccg gcttccgtga accaccccgc aacaaccaca
     2221 cggaggaatt cccatgacaa cccttcaacg cacgcttctc acgttcgcgg cgtttctggt
     2281 gaactatggt ctggtcatct cggtccaccc ggctggtatc gagccgctcg agtgggtcgt
     2341 cgtctggctg atctgtctgc tgatgggcgc cg
//
LOCUS       DAORHD010000806         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54878_length_2370_cov_3.323110, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000806 DAORHD010000000
VERSION     DAORHD010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54878_length_2370_cov_3.323110"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKO06_15665"
     CDS             complement(<1..597)
                     /locus_tag="PKO06_15665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV71140.1"
                     /translation="MDFRLLRYFVAVADQANFSRAAETVHLTQPALSQAIRALEKDLG
                     CVLFDRGRGVRLTPAGERLREHCRQLLPHLQDIRDDLAELHGGLQGTIRPAVLESLLL
                     YLLPPVISRFSSIYPLVKFDFRMQETRDIESSVLSGDCHFGLVSRPPMSRALECQLLE
                     THPHVLVTASANRAPTSQLLQKLPLYLLGDWQAAALEEH"
     gene            705..1580
                     /locus_tag="PKO06_15670"
     CDS             705..1580
                     /locus_tag="PKO06_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HNV71141.1"
                     /translation="MNLQCPCSLNGGSFPLVVVVVCLYFCQVGTLGAWSERGHHLVAR
                     LAEAQMTPSAVASVRRLLNGQSLADVSMWADQIKPSRPETAVYHYANFPARHDQEPFD
                     AQGDNDGAVVPALERSISLLGQAHLPAADRAEALRFVVHLVGDVHQPLHCAPQGDQGG
                     NKITVRFFGRPRNLHQVWDRDILDSAGISEDIHFAALQRIMAEELTESHTGLPQQWAA
                     ESNRLAVWQAYRIPWNGCLDERYVEPSLRLANRQLIKAGRRLAAVLNDAFSRSEQAGE
                     PAIAHSTLDPAEDRF"
     gene            complement(1584..>2370)
                     /locus_tag="PKO06_15675"
     CDS             complement(1584..>2370)
                     /locus_tag="PKO06_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV71142.1"
                     /translation="RIGLVLNMSTLYPATNAPADVQAAELADAVLNRWFADPILVGSF
                     PAEAERLYRSCGILPVIDAHDRRTLFSTGCDFLGVNYYYPHHASAAAPSTDFHLNTSG
                     DRHEDCRFSIAGLFQFVKNPHGRYTDWGWEIFPDGLYDLLMRAHHCQRGLPLFVTENG
                     IGLQDTLVDGEVDDSARIAFVREHLVAIHRAITDGANVGGYYMWSLMDNFSWINGYKK
                     RYGFLHVDRQTLARTRKKSSFWFEAIARTNCLKTNENSTSPIA"
BASE COUNT          522 a          637 c          719 g          492 t
ORIGIN      
        1 gtgctcttcg agagccgccg cctgccagtc accgagcaag tacaggggca gtttctgtaa
       61 caactgactg gtgggggctc ggttggcaga agcagtcacc aatacgtgcg ggtgggtttc
      121 gagcagctgg cattccagcg ctcgcgacat tggcgggcgg gaaaccagcc cgaaatggca
      181 gtcgcccgaa agaaccgagg attcgatgtc gcgggtttcc tgcatgcgaa agtcgaactt
      241 caccagcgga tagattgatg agaacctgga tatcacgggc ggtaacaggt acagtaagag
      301 gctttcgaga acggcaggtc gaatggtgcc ctgcagaccg ccatgcagtt cggcgagatc
      361 gtcgcgaatg tcctgcaggt ggggaagcag ctggcgacag tgttcgcgca gacgctctcc
      421 ggccggtgtg agccgcaccc ctcggccccg atcgaacaag acacacccca gatccttttc
      481 gagggcgcga attgcctgac tcagggcggg ttgtgtcaga tgcacggtct cggccgcgcg
      541 agagaaattc gcctgatcgg ccacggccac gaagtagcgc agaagtcgaa aatccataag
      601 tgatacttat aatttatata aaaactatcg atttcacaac cacctgtgga ttatgccagg
      661 atatggacga tgtaagaatg atcagccgat tttcggaggc ttccatgaat ctgcagtgtc
      721 catgctccct caacggtggt tcctttcccc tcgtggtcgt cgttgtttgc ctgtatttct
      781 gtcaggttgg cacgcttggg gcatggagcg agcgtgggca ccacctggtc gctcgtttgg
      841 ctgaggctca gatgacccca tcggctgtgg cctcggtgcg ccgtctgttg aacgggcagt
      901 cactggcaga cgtgtcgatg tgggctgatc agatcaagcc ttcccgccct gagactgcgg
      961 tgtatcacta tgcgaacttt ccggctcgcc atgatcagga gcctttcgat gcacagggcg
     1021 acaatgatgg agccgtcgtg ccagcgttgg aacgcagtat ttctctgctg ggtcaagcac
     1081 atctgcctgc tgccgatcga gctgaagccc ttcgtttcgt cgttcacctg gtcggagatg
     1141 tacatcagcc gctgcattgc gccccgcaag gcgatcaggg ggggaacaag atcacggtca
     1201 gatttttcgg ccgccctcgc aatttgcacc aggtctggga ccgcgacatt ctcgacagcg
     1261 cgggtatcag tgaagatata cactttgcgg cgttgcagcg aatcatggcc gaggaactga
     1321 cggaatcgca cacgggtctg ccgcaacaat gggcggccga atcgaatcgc ctggccgttt
     1381 ggcaggccta tcgcattccc tggaacggct gtcttgatga gcgctatgtc gaaccctcgc
     1441 tgcgtctggc caaccgacag ttgatcaaag ccggtcgccg tctggctgcg gtgctgaatg
     1501 acgcattctc gcggtcggaa caggcaggcg agccggcgat cgcccattca acgcttgacc
     1561 cggcagaaga tcgattctga cgcttacgcg atgggtgagg tggagttctc gttggttttc
     1621 agacagttgg tgcgggcgat ggcttcgaac cagaacgagc ttttcttacg cgtgcgggcg
     1681 agggtctggc gatcgacatg gagaaagcca tagcgctttt tgtagccatt gatccaggaa
     1741 aagttgtcca tgagagacca catgtaatac ccgccgacgt tcgcaccatc ggtaatcgcg
     1801 cggtgaatgg ccaccagatg ctcacgaacg aacgcgatac gagcggagtc atcgacctcg
     1861 ccgtccacga gagtatcctg cagtccgatg ccattttcgg tgacaaaaag tggtaaccct
     1921 cgctgacagt gatgggcgcg catgagtaaa tcgtacaagc cgtcggggaa aatttcccag
     1981 ccccaatcag tataacgacc gtgtggattc ttcacaaact gaaacagacc ggcaatggaa
     2041 aaacgacagt cctcgtgacg atcgcctgac gtattgagat gaaagtcagt cgatggcgct
     2101 gccgctgaag cgtgatgcgg gtagtagtag ttcaccccga gaaaatcgca tccggtcgag
     2161 aacaacgtgc ggcggtcgtg agcgtcaatc acgggaagaa tgccgcagga tcgatagagt
     2221 cgttcagctt cagccggaaa cgaaccgacc agaatcggat cggcgaacca ccggttcaga
     2281 acggcatctg ccagttctgc cgcctggaca tctgcgggtg cattggtggc aggatagagc
     2341 gttgacatat tgagcaccag gccgatgcga
//
LOCUS       DAORHD010000807         2369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54913_length_2369_cov_3.094209, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000807 DAORHD010000000
VERSION     DAORHD010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54913_length_2369_cov_3.094209"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="PKO06_15680"
     CDS             <1..1596
                     /locus_tag="PKO06_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNV71143.1"
                     /translation="THAVVSWKTTEFTNGVIEYGDTETFGATVRETTGQYATLHSLTI
                     PNLKPEKRYYFKIVAARQNRTSETSEVGQFTTISALEDATPPSAPRGIDIALTETPNQ
                     VTVFWVPNTESDLKGYKIYRSELPTTGFTALEAFLAKGNERFTDVGVVTGKKYYYRVT
                     ATDQGGNESSASEIVSMVIPGDLTAQVLWTRANSPYILYGDLTVAAFGKLTIDSGVVV
                     KAGDYDALKRGDPTKIDLRIEGGLVANASGSEAIVFSSVKPTPRAGDWAGIVIAPNDQ
                     EQPLQLVNVEIAYADRALAINSHEGTMIGVNIHDCGMGVQASAVADLALDQFAIKHCT
                     DGLVLTDNMALKVTNSTVFHCRRGLSSSNNASVEYLQNNFLEYVDFGLKLDEQAGTLR
                     VHNNLFVSGTGVALQIVDQVVQVTNNTIDSPTGLRIDKGTPIIEKNFIFSTRSVSGEG
                     LKGIEHLAGDSPLPVFGPNDVYGFPQDKDYVGCASSAGSLASEPYFMKDFGGGSYDYR
                     LRFPFPDNTDLWGILRDQEPTGI"
     gene            1927..2088
                     /locus_tag="PKO06_15685"
     CDS             1927..2088
                     /locus_tag="PKO06_15685"
                     /inference="COORDINATES: protein motif:HMM:NF016828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HNV71144.1"
                     /translation="MVQEEGQGLVEYALIIGLIAVVAIAALTASGGSISGIFGAIGNK
                     LSSTASSIS"
     gene            complement(2145..>2369)
                     /locus_tag="PKO06_15690"
     CDS             complement(2145..>2369)
                     /locus_tag="PKO06_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV71145.1"
                     /translation="LIKPVGVVVLGKNSLAVDATCCRLMGFDPLRVPYLHAAATLFSG
                     IRESEVYHRGENPRKFATPFTCLPQHEAYR"
BASE COUNT          575 a          614 c          657 g          523 t
ORIGIN      
        1 acacatgcgg tcgtgagctg gaagacgacg gagttcacga atggagtcat cgaatacggt
       61 gacaccgaaa cgtttggcgc cacggttcgt gagacgacgg gccagtatgc gactttgcac
      121 agcctgacga ttccgaatct caaaccagag aagcgatact atttcaaaat cgtggcagcc
      181 cgacagaatc gcacatcaga aacttcagaa gtaggacaat tcacgacgat cagcgcgctc
      241 gaagatgcca cgccgccgtc ggctccgcgg ggaatcgaca ttgccctgac cgaaaccccg
      301 aaccaggtaa ccgtgttctg ggttcccaac acggaaagtg atctgaaagg gtacaagatc
      361 tatcgcagcg aactgcccac cacaggcttt actgcgctcg aagcatttct ggcaaaaggg
      421 aacgaacgat ttaccgatgt cggagtcgtc accggcaaga aatactatta tcgtgtgaca
      481 gcaacggatc agggagggaa cgaaagcagc gcttctgaga tcgtcagcat ggtcattccc
      541 ggtgacttga ccgctcaggt gctttggacg cgggctaaca gcccctacat cttgtacggt
      601 gaccttacgg tcgcggcgtt cggcaaactg accatcgatt ccggggtggt cgtgaaagcc
      661 ggagactatg atgcactcaa acgtggcgat ccgaccaaaa ttgatctgcg catcgaaggc
      721 ggtctggttg ccaatgccag cggttccgag gcgatcgtgt tcagttccgt gaagccgacg
      781 cccagagccg gcgactgggc cggaatcgtg attgcaccga acgatcagga acaaccgctg
      841 cagctggtaa atgtcgaaat tgcgtatgca gatcgggcac tcgcgatcaa cagtcacgaa
      901 ggaaccatga tcggggtaaa cattcatgac tgcggaatgg gtgttcaagc cagtgccgtt
      961 gcggatctgg ctctcgatca attcgcgatc aagcattgca ccgatgggtt ggtattaacg
     1021 gacaacatgg ccctcaaggt caccaattcg acggtgttcc attgtcggcg cgggttgagt
     1081 tcatcaaaca atgccagtgt cgagtatctg cagaacaact tccttgagta cgtcgacttt
     1141 ggtctcaaac tcgatgaaca ggcaggaact ctgcgagtac acaacaatct gtttgtctct
     1201 ggaactggtg tggcattgca gatcgtcgat caggtggtac aggtgacaaa caacacgatc
     1261 gacagtccga cagggctgcg gatcgacaag ggaaccccga tcatcgaaaa gaatttcatc
     1321 ttctcgacgc gtagtgttag cggagagggt ctgaagggta tcgaacatct tgcgggagac
     1381 tctccgcttc ctgtcttcgg cccgaatgac gtgtacggtt ttccgcagga taaggactac
     1441 gtcgggtgtg cgtcgtcagc aggaagtctg gcaagcgaac cttactttat gaaggacttc
     1501 ggcggtggct catacgacta tcgtctccgg ttccccttcc cagacaacac agatctgtgg
     1561 ggcattctgc gcgatcagga acccacaggt atctagagtt ttgcttcagg gaaaacctgg
     1621 tggggtagtt ttgcctcgca tggaacgaga ataccccaag cttgtacacg cggaatccgc
     1681 cttctggttc ggtcgctcgc tgtcctattg aaccacaaac gggccacttt catcgaggaa
     1741 cagagcggtt ccctctgact ggtacacctc tgagatccat cttttgggcg tggggtctgg
     1801 aaactgcatt ttccgctctt ctgtaaaaaa gaaatgattt ttcttggctg gcataacgat
     1861 tgctgatata atttgcgtgt ccgaccaaaa aatctccagg aggtatctga aatgatccgc
     1921 aattttatgg ttcaggaaga aggccagggc ctcgttgaat acgctctgat cattggtctc
     1981 atcgccgtcg tcgctatcgc tgctctgacc gcttccggcg gcagcatcag cggaatcttt
     2041 ggcgccatcg gcaacaaact gagcagcacc gccagcagca tcagctaatc gtttcgctgc
     2101 aaacacgggg aacttcggtt ccccgttttt tttgtctctt aaagtcatcg ataggcttca
     2161 tgctgcggca ggcatgtgaa cggggtggca aatttccggg gattttcgcc gcggtggtac
     2221 acctctgact cacgaatgcc cgaaaagagc gttgccgctg catggagata gggtacgcgc
     2281 agaggatcaa accccatcaa acggcaacag gtggcgtcga cagccagaga gttttttccc
     2341 agaacgacga cgccgacggg cttgatgag
//
LOCUS       DAORHD010000808         2368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54938_length_2368_cov_3.860787, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000808 DAORHD010000000
VERSION     DAORHD010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54938_length_2368_cov_3.860787"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..>2368)
                     /locus_tag="PKO06_15695"
     CDS             complement(142..>2368)
                     /locus_tag="PKO06_15695"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV71146.1"
                     /translation="SRQLQLRHGHTALKRFLMTCTNQLLHELRGKGIRGFMGMPRLVW
                     RTSGAIYLMVLEALRGVRLNLLSLENIMHSQVMLDILAITLLVRYTGSTESPLPVLLF
                     VPIIVAGAVLGFYQGTLYAMVGASAYLLLGLLVNLKALTHVKFYGPQFGDLSQSVGWT
                     VSSYAIMAVALIGSAYLAHHLTAVFKERIFFLNQLLEKNRRESNAQAAVAENVGNAWF
                     ILDPQGNILKYKRGSASFFPTTLTGRCIFEAVSSFKQSGMEYLVQSVLTGGKQRDMER
                     VSVVSPEGTSHVVCCRLMPMVDADGRALVLLIVEDLSELLYFKEHVTQLRQNLEHNKL
                     ELEKVTLDAREANQQLMKSLKTANERSLEIQTLQQTIQQLETTVTGHENEISQLKNEL
                     AAVKSHNDQLTAELRYKHLILEEITELLAHCTNLDELTNLVEQRTRSLFKVDSASLHV
                     FQAAPNAARLTELLDTRRVSPRLLDLPRRNPKVLEPVLNDGKPVIIRAEVRPDKAASM
                     AITNGQSHRLVAYLPVRHEGEVLGMMMLERFGPEENTDRMMEMLIYYLNHTASALKNA
                     LVTRSLEAQRDQLGRTIENLEAQMNLLTEMIQFDPPKEELPFQTFLSRLCVAHGANDG
                     VLLRFHNDGSSQVLARIEQNRAKELLAIEEQVMKALVANPTHKASMQGPDGTTLNAWP
                     LKQGARLVGILFLHVTVQEQVNTSLLDVCARMVEEHLALFVLREEKELWENFYQETLQ
                     V"
BASE COUNT          513 a          687 c          649 g          519 t
ORIGIN      
        1 actccgctcg aagtcggtgt tggccgtttc gtcgactggt actgcgattt ctacaaaatc
       61 aaacgctaac cccgcacaaa aagaaagctc gccgtttccg cagagaatcg gcgagcttct
      121 gaagcgtatg taccgggcgg gttaaacctg cagagtctcc tggtaaaagt tttcccagag
      181 ttctttctct tcgcgcagga cgaagagggc cagatgctct tcgaccatgc gggcgcacac
      241 atcaagtaat gaggtattca cctgctcctg caccgtgacg tgcaggaaga gaattccgac
      301 cagtctcgca ccctgcttca acggccaggc attcagggtc gtaccatcgg gcccctgcat
      361 cgaagccttg tgcgtcgggt ttgccacgag cgccttcatg acctgctctt cgatcgccag
      421 cagttctttc gcccggttct gctcgattcg cgccagcacc tgcgatgatc catcattgtg
      481 gaaacgcagc agaaccccat cgttggcacc gtgcgcgacg cacaggcgac tgaggaatgt
      541 ctggaacgga agttcttctt tcggtgggtc aaactgaatc atctcggtca gcaggttcat
      601 ctgcgcctcg aggttctcga tcgtgcgtcc gagctggtct cgctgggctt ccagactgcg
      661 cgtgaccagc gcattcttca aagccgatgc cgtgtgattg agatagtaga tcagcatctc
      721 catcatgcga tcggtgtttt cttcgggtcc gaaacgctcc agcatcatca ttccaagcac
      781 ttcgccctcg tgccgcaccg gaagatacgc caccagtcgg tgagactgtc cgttggtgat
      841 cgccatcgat gccgccttgt ccggacgcac ttcagcgcga atgatcaccg gcttgccatc
      901 attcaggacg ggttcgagaa ctttgggatt gcggcggggc agatcaagaa gtcgcggcga
      961 aacccggcgc gtatcgagca gttcggtcag acgggctgca ttgggagccg cctggaacac
     1021 atgtagcgaa gcgctgtcga ctttgaacaa cgagcgtgtg cgctgctcga ccagattggt
     1081 caattcatca agattcgtgc agtgcgccag cagttcggtg atctcttcca gaatcaggtg
     1141 tttgtaccgc agctcggcag tcagttgatc gttgtgcgac ttcacggctg caagctcatt
     1201 cttcagctgc gagatctcgt tttcgtgacc agtcaccgtg gtctcgagct gttgaatggt
     1261 ctgttgcagc gtctgaatct ccaacgaccg ttcattggcg gtcttcaaac tcttcatcag
     1321 ctgctggttc gcttcacgcg catcgagcgt gaccttttcc agttcgagct tgttgtgctc
     1381 gagattctga cgcagctgcg tcacatgctc cttgaaatac agcagttccg agaggtcttc
     1441 aacaatgagc agcaccagtg cccgaccatc ggcatcaacc atgggcatca accgacagca
     1501 gaccacatgc gacgttccct cgggcgaaac gacactgacc cgctccatgt cccgctgttt
     1561 gccaccagtc agaaccgact gcaccagata ttccatgccg ctttgcttga agctggatac
     1621 cgcttcgaag atgcagcggc cggtcaaggt cgtggggaaa aagctggcgg agcctcgttt
     1681 gtatttcagg atgttgccct gtggatcgag aatgaaccag gcgttcccga cattctccgc
     1741 aaccgcggcc tgggcattcg actctcggcg attcttctcg agcagctggt tcagaaagaa
     1801 gatgcgctct ttgaagaccg cggtcagatg atgggcaaga tacgcagaac cgatcaatgc
     1861 caccgccatg atggcataac tcgaaacggt ccaaccgaca gattggctca gatcgccaaa
     1921 ctgtggccca tagaatttca catgcgtcag agctttcaga ttgaccagca ggccgagcag
     1981 caggtaggca ctggcgccta ccatggcata caaggttccc tgatagaaac ccagcacggc
     2041 accagccacg atgatcggca caaacagcag caccggcagc ggactttccg ttgagccggt
     2101 gtaacgaacc aacagggtga tagcgagtat gtccagcatc acctgactgt gcatgatgtt
     2161 ttccagcgac agcaggttca ggcgtacgcc acgcagcgct tcgagcacca tcagatagat
     2221 cgcgccggag gtgcgccaga cgagtcgcgg cattcccatg aaaccgcgaa tgcctttgcc
     2281 ccgcagttcg tgcagcaact gattggtaca agtcatgaga aagcgtttca gcgcggtatg
     2341 gccgtgacgc agctgcagct gacgcgac
//
LOCUS       DAORHD010000809         2367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_54979_length_2367_cov_2.914360, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000809 DAORHD010000000
VERSION     DAORHD010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54979_length_2367_cov_2.914360"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PKO06_15700"
     CDS             <1..365
                     /locus_tag="PKO06_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV71147.1"
                     /translation="FLVFARTFPDLQAALVVGRTREAVLGRVSELSQHLLLMVVAAVV
                     LAMAVSLVLADHFITQILKLIEGIRKVGDGVLSWRIPEVSDDELGDAGRAFNTMADRL
                     ERNRLIEDLWRQRERPDA"
     gene            362..>2367
                     /locus_tag="PKO06_15705"
     CDS             362..>2367
                     /locus_tag="PKO06_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71148.1"
                     /translation="MRPLSAFPVLAGFFFFLRAFDTFLILSFSSLFLESFGPAGWPWY
                     FVGFSILFITTQSLLLDRPHLHGEPFLRRALPPLFLTALLLAVFFPTPSGWITFPALV
                     LFRSWDLHATQAFYDLVGKTWALQEARLHLPSLLAAGTLGSVLAGFSLRFLLPSTGFF
                     PLLVLIPFAGCAAWLTLKAYSPDTSTSSSSERPSASPIHWKQISPTHRRYSLAVFLLS
                     IMGSLVVNLVDFQFNANLPDLYAGVTDRAAQVAALLGTLNAVIELTTLALQGLVGRWI
                     FTRLSLGTILSVRPVLLGLLGFIAWAMPQFWTLTGFQFLSRTSTFVFMSPVWVLLLEP
                     LPFSTRSYVRRLLNLVDCITLTSLGLSLALWSNGGSTAHPSLFLLISGFALTTLWFNR
                     SLLSLYPEMIRETLIDLSDSPGMNLDGLKLLSREARRTILRRLFDENGREVQLKVLLR
                     LGPLDLPELHELIHERLPSMTDSTLVASSVRLLLRSSEQVQPLASYLGTCPNPRRLSE
                     IMDAADQTTHPDFEWMFASYFDHQHHRVRGSAVLAYLRHGRGRRLIPQAIETLAGLQQ
                     GHAPRDRSTAAVVMGRLGFPAFTPSLFRLVDDPSDDVARCAYHALASIGTPPVLEFVG
                     QRLSQSGIRGTFARTCWNELAGQAQVSLLQSLAAMSDEERHRTGV"
BASE COUNT          366 a          799 c          613 g          589 t
ORIGIN      
        1 catttctcgt gtttgcgcgc acctttcccg atctgcaggc cgctctggtc gtcggtcgca
       61 cccgagaggc ggttcttggt cgcgtcagtg aactttcgca gcacctgctt ctgatggtcg
      121 tggcggccgt ggtcctggca atggccgtca gcctggttct ggcagaccac ttcatcacac
      181 agatcctgaa actgatcgaa ggcatccgca aggtcggcga cggcgtgctc tcctggcgca
      241 ttcccgaagt cagcgatgac gaactcggcg acgcagggcg agccttcaac accatggccg
      301 atcgactcga acgcaatcgg ttgatcgaag acctgtggcg ccagcgcgaa cgtccggacg
      361 catgagaccc ctttcggcat ttccggttct tgccggcttc ttcttctttc tgcgcgcctt
      421 tgacaccttt ctcatcctct ccttttcctc gctgtttctc gaatcgttcg ggcccgcggg
      481 atggccctgg tatttcgtcg gtttctcgat tctgttcatc accacccaga gtctgctgct
      541 cgatcgtccg cacctgcacg gcgaaccctt tctccgtcgg gctctgccac cgctctttct
      601 gacggctctg ctcctggccg tcttctttcc cacgcccagc ggctggatca cttttcctgc
      661 tctggttctc tttcgcagtt gggatctgca cgccacccag gcattctatg atctggtcgg
      721 aaaaacctgg gctctgcagg aagcgcggct gcatctgcca tcgttgctgg cggccggcac
      781 cctcggctcc gttctcgccg gcttcagcct gcgtttcctg ctcccatcga ccggcttctt
      841 tccgctcctg gtgttgattc ccttcgccgg atgcgcggcc tggctgaccc tgaaagccta
      901 ttctcccgat acttcaacat cctccagttc cgaacgaccc tctgcctctc caatccactg
      961 gaagcagatc tcccccacac atcgccggta ctctctcgcc gtgttcctgc tttccatcat
     1021 gggcagcctc gtggtcaacc tggtggattt tcagttcaat gccaacctcc ccgatctcta
     1081 tgccggcgtg accgatcgcg ccgcacaggt cgcggcgctg cttggcacgt tgaacgccgt
     1141 gatcgaactg acaaccctgg ccctgcaggg tctggtcggt cggtggatct tcacccgcct
     1201 gtcgctggga accattcttt ccgtgcgtcc cgtgctcctg ggtctgctcg ggttcatcgc
     1261 ctgggccatg ccccagttct ggacgctcac gggctttcaa tttctgtcac gcacttccac
     1321 ttttgtcttc atgtctccag tatgggtctt gcttctggag ccacttccgt tctcgactcg
     1381 ctcgtacgtt cgccgcctgc tgaacctggt cgactgcatc actctcacgt cgctcggtct
     1441 gagcctggcc ctgtggagca atggagggag caccgcacac ccatctctct ttctcctcat
     1501 ttcgggtttc gccctgacaa ccctctggtt caatcgctct ctgttaagcc tgtatccgga
     1561 aatgatccgc gaaactctta tcgatctgtc cgattcacct ggaatgaatc tcgatggctt
     1621 gaagctgctt tcgcgcgaag cacgacgcac gattctgcgc cgcctctttg atgagaacgg
     1681 tcgcgaggtc caattgaagg tactactccg acttggtccg ctcgacctgc cggaactgca
     1741 tgaactgatt cacgaacggt tgccctcaat gaccgattcg accctggtag ccagcagtgt
     1801 tcgtctgcta ttgcgttcat cagaacaagt acaaccgctg gcatcctatc tcggcacctg
     1861 tcccaatccg cgtcgcctgt cagaaatcat ggatgccgcc gatcagacaa ctcatcccga
     1921 tttcgagtgg atgttcgcgt cgtatttcga tcatcagcac catcgggtgc gcggcagcgc
     1981 cgttctcgcg tatctccgcc atggccgcgg gcggcgattg attccacagg ccatcgaaac
     2041 gttagccggc ttgcagcagg ggcacgcacc tcgcgatcgt tcaaccgccg cggtggtcat
     2101 gggccgcctc ggatttcccg cgttcacgcc ctcactgttt cgtctggtcg atgatccgag
     2161 cgatgacgta gctcgttgcg cctaccatgc cctggcctca atcggtactc ctccggttct
     2221 ggaattcgtc ggccagcgac tttcgcagag cggcatccgc ggcaccttcg cacgcacctg
     2281 ctggaacgaa ctggctggac aggctcaggt gtcactgctg cagtcgctcg ccgccatgtc
     2341 tgacgaagaa cgacaccgca ccggggt
//
LOCUS       DAORHD010000810         2366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55006_length_2366_cov_3.211597, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000810 DAORHD010000000
VERSION     DAORHD010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55006_length_2366_cov_3.211597"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1522
                     /locus_tag="PKO06_15710"
     CDS             161..1522
                     /locus_tag="PKO06_15710"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HNV71149.1"
                     /translation="MTTTTSDWTKNSWRSFPALQQPVWPEAAVRDESLAKLARLPALV
                     FAGESRSLIQQLADVAAGKAFVLQAGDCSEDFSRCHGPHIHDLLKVILQMSIILAYSG
                     EKKVVKIGRMAGQYAKPRSSDTETVNGIEIPSFRGDMINSAEPTLKARTPDPRRVVEG
                     YFRAAATLNLVRAFTKGGYAGLNHLHSWSVEHFPAYTAKQKYEEIIKGIRKAISFTNA
                     LGLDTSVPQINETTIYTSHEALLLEYEEALTRVDTTTGLWYDTSAHMLWLGDRTRQVH
                     GAHVEYLRGICNPLGVKIGPKHDLDDIKRLIEKLNPENKPGRLSLIVRMGAEKVATLL
                     PPLIHAVQAEGFHVVWLSDPMHGNTYVNSRGQKTRHFETILQEIREFWNVHHAEGTNA
                     GGVHIELTGENVTECVGGSAQLRDKDLELNYQTSCDPRLNPEQAVELAFELSQILNPT
                     HRL"
     gene            1556..>2366
                     /locus_tag="PKO06_15715"
     CDS             1556..>2366
                     /locus_tag="PKO06_15715"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV71150.1"
                     /translation="MTQDRTDTPLKLGFVGGSISSAVGRAHAVACVMDRRFELTAGCF
                     SEVHETNRHTAIEYGVSEKRLYGTWQELLARERGNLDAMVVLLPTPLHHEVVRACLES
                     GFPVICEKALACSSAEAADVLAVTQRLRGFLAVTYNYSGYPMVRELRHRIREGELGTI
                     LHFQVEMPQEGFIKAGESGAVPTPQTWRLHDTLIPTLHLDLGVHLHHLLHYLIGRRPL
                     EVIADHSTYGQFTDVVDYAMGLCRYAEGIRGQIWFSKAALGHRNGLRLRVFG"
BASE COUNT          527 a          615 c          732 g          492 t
ORIGIN      
        1 gacccacaaa atcaaggggc tgcacgagaa cgatttcatc atggcggcga aaaccggagc
       61 actgctcgga tcatgaccgc ttaccgttca cgtttcccgt ggtgtacatc agaaacgtct
      121 acgtgatcgt tttcaatctc ctgaggagtt gatgaacagc atgacaacca caacctcaga
      181 ctggacaaaa aacagctggc ggtcgtttcc ggcgctgcaa cagccggtct ggcctgaagc
      241 ggcagtccgt gatgagtcgc tggccaagct ggcgcgactt cctgcgctgg tgtttgccgg
      301 cgaaagccgg tccttgattc agcagttggc ggacgttgcg gcgggaaaag cgttcgtcct
      361 gcaggcgggg gactgctccg aagacttttc gcgctgtcat ggtccacaca ttcacgatct
      421 gctcaaggtg attctgcaga tgtcgatcat ccttgcctat tcgggtgaga agaaagtcgt
      481 gaagatcggt cgaatggccg gccagtatgc gaaaccgcgc tcgtcagaca cggaaacggt
      541 gaatggcatc gagattccca gcttccgggg tgacatgatt aacagcgcag aaccgacgct
      601 gaaagcacgc acgcctgatc cgcgacgagt cgtggaaggg tacttccggg ctgccgcgac
      661 cctgaatctg gtgcgcgcct tcacaaaagg tgggtatgcc ggtctcaatc acctgcactc
      721 gtggagtgtc gaacattttc cggcatacac cgccaaacag aaatacgaag agatcatcaa
      781 gggaatccgc aaggcgatca gcttcaccaa cgcgctgggg ctggatacca gtgtgccaca
      841 gatcaatgaa acgacgatct acacgtcgca cgaagccttg ttgctggagt atgaggaggc
      901 gttgacgcgt gtcgacacca cgaccgggct ctggtatgac accagcgcgc atatgctctg
      961 gctcggtgat cgcacacggc aggtgcacgg cgcgcatgtc gaatatttgc gtggtatctg
     1021 taatcctctt ggcgtcaaga tcggaccgaa acatgacctg gacgatatca agcgactgat
     1081 cgaaaagctg aatcctgaaa acaagcccgg tcggttgtcc ctgatcgtga gaatgggcgc
     1141 tgagaaggtg gcgactctgc tgcccccgct gattcacgcg gttcaggctg aagggttcca
     1201 tgtcgtctgg ttgagcgatc cgatgcacgg aaacacgtat gtgaacagcc gtgggcagaa
     1261 gacccggcat ttcgagacga ttctgcagga gatcagagag ttctggaacg tgcatcatgc
     1321 cgaggggacc aacgccggag gagtacatat cgagttgacc ggagagaacg tcaccgagtg
     1381 cgttggtggc agtgctcaat tgcgtgataa agatctggaa ctgaattatc agacgagttg
     1441 tgatccgcgc ttgaacccgg aacaggcggt tgagttggct tttgaattgt cacagattct
     1501 caaccccacc caccgtctct gacgggcgcg gagtcgatgc caaggagaag gaaccatgac
     1561 gcaggacagg acagacactc cgctgaaact gggttttgtt ggtggctcga tcagctctgc
     1621 ggtcggcaga gctcacgccg tcgcctgcgt catggatcgc cggttcgagt tgacggccgg
     1681 gtgcttcagt gaggtccacg agaccaaccg gcacaccgcg atcgagtatg gagtttctga
     1741 aaaacgcctc tatggaacat ggcaggaact gctggccaga gaacgcggca acctggacgc
     1801 catggttgtg cttctgccga ccccgttgca tcacgaagtg gtgcgggcgt gcctggagag
     1861 cggattcccg gtgatctgcg agaaagcgct ggcatgttcg agtgcggaag cggccgatgt
     1921 gctggcggtg acgcagcgac tgagagggtt cctggccgtc acgtataact attccgggta
     1981 tccgatggtg cgcgagttgc ggcatcggat cagagaggga gagttgggaa ccattcttca
     2041 ctttcaggtg gaaatgccgc aggaaggttt tatcaaagca ggcgagagcg gtgccgtgcc
     2101 cacgccgcaa acgtggcgtc tgcacgatac cctgattccg acgctgcacc ttgatctggg
     2161 ggtgcatctg catcatctgc ttcactatct gatcggtcgt cgcccgttgg aagtcatcgc
     2221 tgaccatagc acgtacggcc agttcaccga tgtggtcgat tatgccatgg ggctgtgtcg
     2281 ttatgccgag ggaattcgtg gccagatctg gttttcgaag gctgcactgg ggcaccgtaa
     2341 tgggttgaga ctgagagtct ttggta
//
LOCUS       DAORHD010000811         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55113_length_2363_cov_2.905113, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000811 DAORHD010000000
VERSION     DAORHD010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55113_length_2363_cov_2.905113"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PKO06_15720"
     CDS             <1..382
                     /locus_tag="PKO06_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71151.1"
                     /translation="RVEGVRESQNTLCLIRSMLRKGLVDTFGLSITTPYPGSDLYDLV
                     KKWNVLRSEFDGNWEKWNNVWDYALQLPGILPHDVTRLKVLGSVMQALALLKSGTTNW
                     KSLHFLFGRSWELMTRTVRTICPW"
     gene            complement(429..1751)
                     /locus_tag="PKO06_15725"
     CDS             complement(429..1751)
                     /locus_tag="PKO06_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71152.1"
                     /translation="MSDIVLINPRTSLNIENNEPLNLLSLAAYLEAKNLTASIFDEFR
                     PLTSLEEALRQTRYIGITSNTCSYPRAVELVQHIKRYFPHVRTIAGGVHPTTRPDDAF
                     RDGFDIVVVGEGERALVRILSEAITEGLVHGTEVSEQELYRPARHLIDMPFYARTKDR
                     CPHDPNLNFMSRGTRMACYLTSRGCPFQCIFCHNIWRHSKVRFVETDRVIEELTSLKT
                     TYQVEAVWLMDDHIFLHKSRAIDLFQKMIASRLNLIWASAARVDSLSDELLELAYRAG
                     CRRLSLGIESGSQRILDRISKGTTVEANARAIAMCKKHNIRALATIMIGNPDETLSDL
                     RATQAFIRRTQPDDLAVSILTPFPGTLLWKQCEEKGLIPSHPVFSQFNYLKAPVQLSC
                     FISPERLEVYKRRILTRYYLHPSRLSPFLGKVLSNPWSMFHKVSEYFL"
     gene            complement(1748..>2363)
                     /locus_tag="PKO06_15730"
     CDS             complement(1748..>2363)
                     /locus_tag="PKO06_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV71153.1"
                     /translation="HIVDFWRSRCPRFVFVVKHGKEELRTYIETLGIAAEFTEPRDLR
                     GIADGLMSARSRLTDRFVVILGDCLFQGHFHLPPDLRLGLGIQRHADAESIHRSYLVE
                     LDDSESRIRHVEEKPKILKNNLLGLGFYFLDTRVFPYIERTPPSSLRNEVEITDVMEH
                     MIKAGEPFSPVFFEGGYLNVTYAEDIDRAGVLLLSKDPPKGHTS"
BASE COUNT          537 a          574 c          695 g          557 t
ORIGIN      
        1 gcgggttgag ggcgtgcggg agtctcagaa cacactctgt ctcattcgaa gcatgcttcg
       61 gaagggtctg gtggacacct ttggactttc tatcacgact ccgtatccag gaagtgatct
      121 ctacgattta gtgaaaaagt ggaatgtgtt gcgctctgag ttcgatggta actgggaaaa
      181 gtggaataac gtctgggact atgcattgca gcttcccggc atccttcctc atgatgtgac
      241 tcgcctcaag gtgctcggtt ccgtcatgca ggcattggca cttctcaaga gtggtaccac
      301 gaactggaag agcctgcact tcctgttcgg aagatcgtgg gaactgatga cacgcacggt
      361 caggacaatc tgtccctggt gaccttgatt cgtgacgcag gtgagcctgc gctttgcccc
      421 cccccttgtt acagaaagta ttccgagacc ttgtgaaaca tggaccacgg attcgacaac
      481 acttttccga gaaagggaga gagtcgggat gggtggaggt agtatctggt gaggatgcgt
      541 cgcttgtaca cctcaagacg ctccgggctg atgaagcaac tgagctgaac gggagctttg
      601 agataattga attgtgaaaa caccggatga ctcggaatga gacctttttc ctcgcattgc
      661 ttccacagaa gagtcccggg aaaaggagtc aagatgctca cggcaagatc gtcaggctgc
      721 gtgcgacgaa tgaaagcttg ggtggcacgg aggtctgaga gggtttcgtc gggattcccg
      781 atcatgatcg tggcgagggc tctgatgttg tgtttcttgc acatggcgat tgcccgcgca
      841 ttcgcttcca ccgtcgttcc tttgctgata cgatcgagaa tgcgctgact gccagactcg
      901 atgccgagag aaagacgtct gcaacctgcc cgataggcca gttcgagcaa ttcgtcggat
      961 agagagtcta ctcgcgccgc tgatgcccag atgaggttga gccgactggc gatcattttc
     1021 tgaaagaggt cgatggcacg agacttgtgg agaaaaatgt ggtcatccat gagccagaca
     1081 gcctctacct gatatgtcgt cttgagagaa gtcagctcct cgatgacgcg gtcggtctcg
     1141 acaaatcgga ccttcgaatg cctccagatg ttatggcaga agatgcactg aaatgggcac
     1201 cccctggatg tcaggtagca ggccatgcga gttcctcgtg acatgaagtt caggttcgga
     1261 tcgtgtggac accgatcttt ggttcgggcg taaaacggca tgtcaatcag atgtcgggct
     1321 gggcgataca gctcttgttc gctgacctcg gttccatgaa cgagaccttc ggtgatggct
     1381 tcagacagga tgcgcacgag cgcgcgttct ccttcgccga cgacgacgat atcgaatcca
     1441 tctcggaacg cgtcgtccgg gcgagtggtg ggatggaccc cgccggcaat ggtcctgacg
     1501 tgaggaaagt agcgcttgat gtgctgcacc aactccaccg cacgagggta cgaacaggtg
     1561 ttggaggtga tgccaatgta gcgggtttgg cgcagggctt cttccaggct ggtgagaggt
     1621 ctgaactcgt cgaaaatgga agccgtcaag ttctttgctt ccagataggc cgccaatgaa
     1681 agcaggttga gtggttcatt gttctcgata ttgagacttg tccggggatt gatcagcacg
     1741 atatcactca tgatgtatgc ccctttggag gatctttgga caaaaggagg acacccgccc
     1801 tgtcaatgtc ttcggcatag gtcacgttca gatatccccc ttcgaaaaat acaggggaaa
     1861 agggctctcc agccttgatc atgtgttcca tgacgtcggt gatttcgact tcgtttcgta
     1921 gcgatgaggg tggggttctc tcaatgtacg ggaagacgcg ggtgtcgaga aaatagaagc
     1981 cgagaccgag caggttgttt ttcagaatct tcggcttttc ttcgacatgc cgtattcgcg
     2041 attctgaatc gtccagttcg accagatagc tacgatggat ggactctgca tcggcgtgcc
     2101 gctgaatgcc aagtccgagt ctcaaatccg gcggcagatg aaagtggccc tgaaacaaac
     2161 agtctccgag aatcaccaca aatcgatcgg tgagacgtga acgtgcggac atgaggccat
     2221 cggcgattcc tctgagatct cgaggctcgg tgaactcggc tgctattccc agagtttcaa
     2281 tgtaggtgcg caactcctcc ttgccgtgct tgacgacgaa gacgaatctc gggcatcgtg
     2341 agcgccagaa atcaacgatg tgc
//
LOCUS       DAORHD010000812         2359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55249_length_2359_cov_4.375868, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000812 DAORHD010000000
VERSION     DAORHD010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55249_length_2359_cov_4.375868"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PKO06_15735"
     CDS             complement(<1..1226)
                     /locus_tag="PKO06_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71154.1"
                     /translation="MALVVCRPEEKRALIWSFLYFFCLMAGYYAIQPLRDEIGVAGGL
                     DQLSWLFIASMAIMVAANPIFSLISARWPRRIFIPWVNRFFIVNLLLFFAGMNMAPEV
                     WQLWIGRCFFLWVSIFNLFVVSVFWSFMADRFTSEQGKRLFGCIGAGGTVGQIAGSAC
                     AAWLTPLIGRYALLLIAALLLEATLKATRELTAGDEAQPSREQPSVESATPSGVRSPS
                     PAAGETGTATRWQSAIDGFRKVVASPYLLGICLFILLYTFTSSLLYFNKAVIVKEALA
                     SPDQRTAFFGRLNTWVATVTLALQLLVTGSVLPVLGVGLCLSLVPFITILGFGGLYYA
                     PGLMIFALVEVTRKAANYAIARPAREVLFTVVSRDEKYRAKNLIDTVIYRGGDALAAA
                     LFGVVALNIYFLLPEWT"
     gene            complement(1226..>2359)
                     /locus_tag="PKO06_15740"
     CDS             complement(1226..>2359)
                     /locus_tag="PKO06_15740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HNV71155.1"
                     /translation="AARRFDVAVIGGGPAGGSAAWQLARSGRSVVICEKESLPRYKPC
                     GAGISRQLAGWFDFSLEPVINRRIRQVRFTWCGGDPIALELPEDQALWLTRREPFDML
                     LLQEAQRHGANLLAPCRVTAVTREPEGWLLQTDTGPISASWVILAEGAKGTLSAPLGF
                     STRRFIRGAALEAEIPHSHPTDTAVVDFGRVKFGYGWVFPKADGYSIGIGSFKGGEEP
                     NLVKLLEELVRESGLDLAQGKWFGHPVLLWDGDFDLHADRALLVGEAAGCVDPFTAEG
                     IRWAAFTGLRAAESVTKCLNGNYEAASEYTKLVQKEFGSEMNWARKLAAAYFHLGKLA
                     YKGIVKRPGAGLAMMRLLSGDLRYSQVAERALARLNPLNWLRF"
BASE COUNT          547 a          756 c          669 g          387 t
ORIGIN      
        1 gtccactcgg ggagcaggaa gtagatgttg agcgcgacca cgccgaacag cgcggctgcc
       61 agagcatcac cccctcgata gatgaccgta tcgatcagat tcttggcccg gtatttctcg
      121 tctcgcgaaa ccacggtaaa cagcacttcg cgagccggtc gcgcaatcgc atagttcgcg
      181 gccttgcgcg tcacttcgac gagggcgaaa atcatcagtc ccggcgcgta gtacaagcca
      241 ccaaagccaa gaattgtgat gaatggaacg agagacaggc aaagaccaac tcccaacacc
      301 ggaaggaccg aaccggtcac caagagctgc aacgccaggg tcaccgtcgc gacccaggtg
      361 ttcaggcgac cgaagaaagc cgtgcgctga tcgggactcg ccaacgcttc cttcacgatg
      421 acggccttgt tgaaatacag cagcgacgag gtgaaggtgt agagcagaat gaacagacag
      481 atgcccaaca gataggggga cgccaccacc tttctgaacc cgtcgatggc actctgccat
      541 ctggtcgccg taccggtctc gcctgcagct ggcgacggcg aacggacccc gctcggcgtc
      601 gcagactcga cgctcggctg ctcgcgcgac ggctgtgctt cgtcaccagc ggtgagttcg
      661 cgagtggcct tcagcgtggc ttcgagcaat aacgccgcaa tcagaagcag ggcgtaccga
      721 ccgatcagcg gggtcagcca ggcggcacat gctgatccgg cgatctgtcc cacagttcct
      781 cccgcgccga tgcagccgaa cagccgcttt ccctgttcag aggtaaaccg atccgccatg
      841 aagctccaga acacggagac gacgaacaga ttgaagatcg agacccagag gaaaaaacag
      901 cggccgatcc agagctgcca gacttctgga gccatattca tgccggcaaa aaacagcaga
      961 agattcacga tgaagaagcg attgacccag ggaatgaaga tccggcgcgg ccatcgcgca
     1021 ctgatcaggc tgaagatcgg attcgccgcc accatgatcg ccatcgaggc gataaacagc
     1081 cacgacagct gatcaagacc gccggccacg ccgatctcgt cacgcagcgg ctgaatcgcg
     1141 tagtatcccg ccatcaggca gaaaaagtag aggaaagacc agatcagcgc ccgcttttct
     1201 tccggtcgac acacgaccag cgccattaga agcgcagcca gttcagcggg ttcaggcgag
     1261 ccagggctcg ttcagcaacc tgggagtacc gcagatctcc ggaaagcagg cgcatcatgg
     1321 ccagccctgc cccgggcctt ttcacgatgc ctttgtaggc cagtttgccc agatgaaagt
     1381 aggcagcagc cagttttcga gcccagttca tttcggagcc gaactctttt tgtactagtt
     1441 ttgtgtattc tgaagccgct tcgtaattgc cattcaggca cttcgttacc gattcggcag
     1501 cccgcagccc cgtaaacgcc gcccagcgaa tcccttcggc agtgaacgga tcgacgcaac
     1561 cggcggcttc cccgaccagc agggcccgat cagcgtgcag atcgaagtcg ccatcccaca
     1621 gcagcaccgg gtgcccgaac cactttccct gagccaggtc gagacccgac tctcgcacca
     1681 gttcctccag cagcttcacc agattcggtt cttcaccacc cttgaacgag ccgataccga
     1741 tcgagtagcc atcggctttg ggaaacaccc agccatagcc gaacttcacc cggccgaaat
     1801 cgacaacggc ggtgtctgtc ggatgcgagt gcggtatttc cgcttccaga gcagctccgc
     1861 gtatgaaacg acgggtacta aagcccaacg gggcactcag cgtgcctttc gcgccttccg
     1921 cgagaatgac ccaggaagcg gagatcggcc cggtatcagt ctgtagcagc cagccttcag
     1981 gctcacgcgt caccgcggtc acacgacagg gagcgagaag attcgctccg tgacgctgcg
     2041 cttcctgcag cagaagcata tcgaacggtt cgcggcgcgt cagccagagt gcctggtctt
     2101 cgggcagctc gagtgcgatc gggtcgccac cgcaccaggt gaagcgcacc tgccgaatgc
     2161 ggcgattgat cacgggttca agcgaaaaat caaaccaacc ggccagctgc cgcgagattc
     2221 cggctccgca gggtttatac cgcggcagcg actctttctc gcagatcacc accgagcgac
     2281 cggaccgagc cagctgccag gcagcgctgc cacctgccgg accgccgcca atgacggcga
     2341 catcaaagcg acgcgcggc
//
LOCUS       DAORHD010000813         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55376_length_2356_cov_3.767927, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000813 DAORHD010000000
VERSION     DAORHD010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55376_length_2356_cov_3.767927"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..>2356)
                     /locus_tag="PKO06_15745"
     CDS             complement(109..>2356)
                     /locus_tag="PKO06_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71156.1"
                     /translation="NRDILLREARAEPGRDIWPLMSVCHPAFSGVSTHPTYLYPLPNP
                     LLQPRERAPATTASGLEPTPTLTTTVDAATLQHPSRQALPLLALLTFAWLFLTASLFT
                     RLNRLSLIPRLSLREKLLVVLGFSIVPPLLALLLVCTSLFEFWRIEERTRLRQHLQQR
                     LEHLESGFTEFIAEQQQVILREREQLSTVLFAEPRSIGRSLDRFLQITQVPFAFLIRC
                     DGVEFKQDQFVPGLPLLEDESRERLHEVARIMAIRILSEYLTPEQFEAYRSAGPPSFW
                     KRDRLIIATQMWQAREMQYFLGRDVFTLDPSFSGFRNGVIKVVMIDPPADKLGRAPVG
                     GFLCLLQPYLGMGRTHFSRLFQHPNPELLAYDGYDLQAGVFARSMEFGDTDRRLLERF
                     RWLSDSSQGDQLLRLARAGALNPWSPQDEAEEGIISACRTFHSYPFVAVLTAREQLRP
                     VRAVATTLASAAAFLYFWLLVRFSAALISRSFVSPMRQLGEISVAIGQGDVPQQVWLP
                     GTGDLSDLAADLNLMCKGLREGKLLSRFVSSSAQADIANISSERFEPGGELRQSAILF
                     SKVRDFHELSQQLHGAQVIELLNQYFAAMEPAILQAGGVLDKYVGDAIMAVFHGIDGQ
                     PPGIQAVTAARAMRRALSEFNREQKRSGDPEIRSGIGIACGAVISGRIGSRRHRLEFT
                     VIGDVVNTAARIESQRHRVSHSSILLDTVTADLVRHLHPIIELGCIELKGKSRPVDLY
                     ELKTEPME"
BASE COUNT          540 a          620 c          759 g          437 t
ORIGIN      
        1 gctgttcgcg ggtttcgaag gagacgatgg ccatgcgcac caacagaaac ggcacgcaga
       61 gaagcaaaaa ccacatcaga accgattcgc gtggagaaag aaagcgattc attccatcgg
      121 ctccgttttg agttcgtaca agtcgacggg gcgacttttc cccttcaatt caatgcaacc
      181 gagttcgatg attggatgaa gatgccggac gagatcggcc gtcacggtat cgagaagaat
      241 ggaagagtga gagacgcgat gacgttggct ttcgatgcgg gcggcggtat tcacgacgtc
      301 accgatgacc gtgaactcga gtcggtgtcg gcgtgaaccg atgcgaccgg agatgactgc
      361 tccgcatgcg ataccgatgc cgcttcgaat ttcgggatcg ccggagcgct tttgctcacg
      421 gttgaactca gacaaggcgc gtcgcatcgc ccgtgcggcc gtgacggcct ggatgcccgg
      481 gggctgcccg tcgattccgt gaaaaaccgc catgatggcg tctccgacat acttgtcgag
      541 cacgccaccc gcctgcagaa tcgcgggctc catggcggca aagtactggt tgagcagttc
      601 gattacctgc gctccgtgca gttgttgaga caactcatga aagtctctga ccttcgagaa
      661 gagaatcgcc gattggcgga gttctccccc gggttcgaat ctttcgctgg agatgttggc
      721 gatgtcagcc tgagcgctgc ttgaaacaaa ccgcgaaaga agtttccctt ctcgtaatcc
      781 tttacacatg aggtttaggt cggcggccag atcggacaaa tcacccgtac cgggaagcca
      841 gacctgttgc ggaacgtctc cctgcccgat ggcgacactg atctctccca actgccgcat
      901 cggcgagacg aagctgcgag agatgagagc ggccgaaaac ctgaccagta gccagaaata
      961 caggaatgca gctgcgctgg ccaaggtggt cgcgacggcg cggacggggc gaagttgctc
     1021 gcgcgcggtc aggaccgcga caaacggata actgtgaaag gtgcgacagg ccgagattat
     1081 gccctcttcc gcttcgtcct gtggagacca gggattcaaa gcccctgccc gtgcgaggcg
     1141 tagaagctgg tcgccctggg aactgtctga caaccagcga aaccgctcaa gtagacggcg
     1201 atcggtgtct ccaaactcca tggaacgggc aaagactcca gcctgcaggt cataaccatc
     1261 gtaggcgagc agttcagggt tggggtgttg gaacagccgg gagaaatgcg ttcgtcccat
     1321 gcccagatag ggttgaagca ggcatagaaa gccgccgact ggggcacgtc caagtttgtc
     1381 ggcgggtggg tcgatcatga ccactttgat cacgccgttg cggaatccgg aaaagctggg
     1441 atcgagcgta aacacatcgc gtccgagaaa atactgcatc tcacgtgcct gccacatctg
     1501 cgtggcaatg atcagacgat cgcgtttcca gaaagaaggg ggaccggcag aacggtaggc
     1561 ttcgaactgt tcgggcgtga gatactcgga aagaatgcgt atggccatga tacgagcgac
     1621 ttcgtgcagc cgctcgcgtg attcgtcttc gagcagagga agcccgggga cgaactggtc
     1681 ctgtttgaac tcgacaccgt cgcagcgaat cagaaaggcg aagggaacct gcgtgatctg
     1741 cagaaaccga tcgagacttc gaccgatgct ccgtggttcg gcaaacaaga cggtcgatag
     1801 ctgctcgcgc tcgcgaagaa tgacctgttg ttgttcggct atgaactcgg tgaaccccga
     1861 ttcgagatgt tcgaggcgct gttgcagatg ctgtcgcaga cgggtacgct cttcgatacg
     1921 ccagaactcg aacagcgagg tgcagaccag gagcagggcg aggagcggcg ggacgatcga
     1981 gaacccgagg acgacgagca gtttttcccg gagagaaagc cgtgggatca gactcaggcg
     2041 gttcagccgg gtgaagagag acgcggtgag gaacaaccac gcgaaggtaa ggagcgcaag
     2101 aagcggcaag gcttgccgcg atggatgctg cagagtcgca gcgtcaaccg tggtggtcag
     2161 ggttggggtg ggctcgagcc cagaagccgt ggtcgcgggg gcgcgttcgc gcggttgcag
     2221 cagcgggttg ggtaatggat acagatacgt cgggtgtgtc gacacgccac tgaacgcagg
     2281 gtgacacacg gacatcagtg gccagatgtc tcttcctggt tcagcacgtg cttcgcgtaa
     2341 caggatgtcg cggttc
//
LOCUS       DAORHD010000814         2350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55591_length_2350_cov_4.155120, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000814 DAORHD010000000
VERSION     DAORHD010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55591_length_2350_cov_4.155120"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKO06_15750"
     CDS             <1..254
                     /locus_tag="PKO06_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009647073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HNV71157.1"
                     /translation="QAKAAERKAMAEAKEQEMKAYEQEMRAKLVEAEANVPKGIAQAL
                     NEGNIGILDYYALKNINADTEMRRSFSGVGADIGPTARN"
     gene            266..907
                     /locus_tag="PKO06_15755"
     CDS             266..907
                     /locus_tag="PKO06_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71158.1"
                     /translation="MFEFIVFVVFIVISVLSEMSKNKDKKQDEDVDIGSLEDFFKKRP
                     SEPPASQPSSSKRSSEFESLEEPIQGGRFGSSETSFPDHERAGGSEFQTPPGLPPLPI
                     EEPEPRRETRHKQKKGKKHGKPAHQAPPVHVSSEGECLDQAPSLEGASLERLPTSSLQ
                     RTAPSLRLNPTQRRALHISWNRRRFIDAVIMSELLQRYDLNRVFGRLPAVRKD"
     gene            1241..2158
                     /gene="rfbD"
                     /locus_tag="PKO06_15760"
     CDS             1241..2158
                     /gene="rfbD"
                     /locus_tag="PKO06_15760"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNV71159.1"
                     /translation="MNILLTGCNGQLGLEFQKAAGISPFAIVATDQRELDITDEDAVA
                     NLFKPPSFDLVINAAAFTAVDQAEADPDSAYAVNRDGPANLAKICAHHGIPLIHFSTD
                     YVFDGNQHTPYRETDATHPLGVYAVSKDEGEREVRKRLANHLIIRTSWLFSAHGNNFV
                     KTILRLARERELLRVVDDQLGCPTSAKDLAHVALDLAKRVQRRETIPWGTYHYCNRGE
                     CSWYGFTKEILAQGSKHETFKVKEIVPITTDQYPLPARRPPYSVLDTMKYTRAFDQTP
                     PHWQESLGVVLAELYGKKTTRHTQTGAHA"
     gene            2155..>2350
                     /locus_tag="PKO06_15765"
     CDS             2155..>2350
                     /locus_tag="PKO06_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV71160.1"
                     /translation="MKGIILAGGSGTRLYPITRVMSKQLMPIYDKPMIYYPLSTLMLG
                     GIREVLIISTPEDTPLFQRLL"
BASE COUNT          543 a          730 c          630 g          447 t
ORIGIN      
        1 cgcaggcgaa agcggccgaa cgcaaggcca tggccgaagc gaaagaacag gaaatgaagg
       61 catacgaaca ggaaatgcgc gccaagctgg tcgaagccga agcgaacgtt cccaagggta
      121 tcgctcaggc actcaatgaa ggcaatatcg ggattctcga ttactacgcg ctcaagaata
      181 tcaatgccga tacggaaatg cgccgttcct tctccggggt cggcgcggat atcggcccca
      241 ccgcccgcaa ctgacgacct gatccatgtt tgaattcatc gtctttgtcg tgttcatcgt
      301 catttccgta ctttcggaaa tgtcgaagaa caaggacaag aagcaggacg aagatgtcga
      361 catcgggtca ctcgaagact tcttcaagaa gcgtccgtcc gagccacctg cctctcaacc
      421 cagctcttca aaacgatcaa gcgagttcga aagtctcgaa gagccaattc agggcggcag
      481 attcggctca tccgagacct cgttccccga ccacgagagg gccggtggca gcgagttcca
      541 gacccctccc ggactgccgc ctcttcccat cgaagaaccc gagccacggc gcgagaccag
      601 gcacaagcag aaaaaaggca aaaaacacgg caagcccgcg catcaagcac ctcccgtgca
      661 tgtctcgtct gaaggcgagt gcctcgatca ggccccctct cttgaaggag ccagcctgga
      721 gcgacttccg acaagcagcc tccagcgcac cgccccttcc cttcgcctga acccgaccca
      781 acggcgtgct ctgcacattt cctggaaccg ccgtcgcttc atcgatgccg tcatcatgtc
      841 cgaactgctg caacggtatg atctgaatcg agtctttggt cgcctgcccg cggtgcgcaa
      901 agactgaccg ccagtcactc tgtctcccaa ccggtctgct tcggcaggcc ggtttttctg
      961 tccgccggaa acctgcctca cgagggccgt ctgtgggagt ggtctgaggc gagtaggcca
     1021 gtaattcctc tgggtcaggc aaacgtcgta taggcgctgg cagcgtcatt gtattctcct
     1081 gcttgggctt tccccttggc atcgctcaac aattcactcg cgccgagacg aaaactggac
     1141 agagtaaaaa gaccccgtgc cctcatgggg cggcttgacg tgccgcggct ttgcgtgcga
     1201 aaatgcggcg acttcagagt tttgcgttca gggagaggca atgaacattt tgctgaccgg
     1261 atgtaatggc caactggggc tcgagtttca aaaagccgcg gggatttccc cttttgcgat
     1321 tgtcgcgact gatcagcgcg aactcgacat caccgacgaa gacgcggtcg ccaacctctt
     1381 caagccgcca tcgttcgatc tggtcatcaa cgcggcggcg ttcacggccg tcgatcaggc
     1441 cgaagccgac cctgattccg cgtatgcggt caaccgcgac ggaccggcca atctggccaa
     1501 gatctgcgcg catcatggga ttccgctgat tcacttttcg accgattacg tcttcgatgg
     1561 caaccagcac actccgtacc gcgagaccga cgcgactcac cccctgggcg tctatgccgt
     1621 cagtaaggat gaaggcgaac gggaagtacg caagcgactg gccaatcacc tgatcattcg
     1681 taccagctgg ctgttcagcg ctcacggcaa caacttcgtc aagacgattc tgcgtctggc
     1741 gcgagaacgc gaactgctgc gcgtcgtcga tgaccagctg gggtgcccga cctcggcgaa
     1801 agatctggcc catgtcgccc tcgatctcgc caagcgcgtg caaagacgag aaaccattcc
     1861 ctggggcacc tatcattact gcaatcgagg cgagtgcagc tggtacggtt tcacgaaaga
     1921 gattctcgcc cagggttcaa agcacgagac cttcaaggtt aaagagatag taccaataac
     1981 taccgatcaa tatccgctac cagcaaggcg tccgccatac tcagttcttg atacgatgaa
     2041 gtacacccgc gccttcgacc agacgccgcc gcactggcag gaaagtctgg gggtagttct
     2101 cgcggagctg tacggcaaga aaacgacacg tcatacacaa acgggagcac acgcgtgaag
     2161 ggcatcatcc tcgccggagg ttccggaacc cggctgtatc cgatcacccg cgtcatgagc
     2221 aaacaactca tgccgatcta cgacaaaccg atgatctact atccgctctc aaccctgatg
     2281 ctgggcggaa tccgcgaggt gctgatcatt tcgacccccg aagacacccc gctctttcag
     2341 cgtctgctgc
//
LOCUS       DAORHD010000815         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55829_length_2344_cov_3.815203, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000815 DAORHD010000000
VERSION     DAORHD010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55829_length_2344_cov_3.815203"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..711
                     /locus_tag="PKO06_15770"
     CDS             202..711
                     /locus_tag="PKO06_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNV71161.1"
                     /translation="MLSFEIGSPIVHPIHGAGILREIQKREIRGKVMKYYIIDFPYSD
                     LGQVMVPIDMAEKVGLRTITLGNSIDSIFEVLGESFTEAEEEEIKSFHQRYREYLEKI
                     QSGELSQVAYVYRLLFRRSLVKPLGTKDKALFETARQLLVSEIVYAKRISEDDARDML
                     DTAMEEVVF"
     gene            complement(765..1745)
                     /locus_tag="PKO06_15775"
     CDS             complement(765..1745)
                     /locus_tag="PKO06_15775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HNV71162.1"
                     /translation="MDILISRPFPGEGIGRLEQAGHRVRVLADDRPQSEFYAALSQSD
                     AAISLLSDPISAEHLRQCPRLRVLANYAVGFNNIDIAAATAAGIAVSNTPHVLTDATA
                     DVTMILILDALRRVPEALDSLRRGEFTGWKPIYLLGQDLAGKNLGILGMGRIGEAVAR
                     RAEAFGMRILYHTRSGAKPGLPWPAVSFEALLAESDVLSLHCPLTAETTHLIGWNQLQ
                     RMKRTAVLINTSRGPVVDEAALARALREKVIFAAGLDVYEREPIVHPDLLNVPNAVLL
                     PHIGSGTIETRSRMGTMAAECVLAVLAGQLPPYPVNPDVRNHPRWGKGTL"
     gene            1881..>2344
                     /locus_tag="PKO06_15780"
     CDS             1881..>2344
                     /locus_tag="PKO06_15780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71163.1"
                     /translation="MPKVLLDLPFIMMRKPLMFLSLVVLWLLAGGRTYAPNGGLKPPS
                     GEIPRPPSRGRSGEGLGMQYLSKPERTYGYWMARGREYLSRAEHEQAVLAFRKALKER
                     PVSEDAHFLAGIAYERRGRECLPGDATNWDELAEQEYRAAIALSDYLPARYNL"
BASE COUNT          490 a          670 c          701 g          483 t
ORIGIN      
        1 cagccaaaaa ggaaggcaag taattctttg tccctccaac cccggagcca cgttccaggt
       61 gtccgcatct ctggttcgct ccaggtttct catgtcagtg acggccgttg ccccgaatct
      121 cggggcaacg gccggtttgc tctccgtccg gttctcctcg agcccgtgtt ctcaatctgt
      181 tgttagagga gctctcaagg tatgttgagt tttgaaatcg gttccccgat cgtacatccc
      241 attcacggcg ccggcattct gcgcgagatt cagaagcgcg agattcgcgg caaggtgatg
      301 aaatactaca ttatcgactt tccatacagt gacctgggtc aggtcatggt tccgatcgat
      361 atggccgaga aggtgggtct gcgcacgatc accctcggaa acagcatcga cagcattttc
      421 gaagtgctgg gcgagagctt caccgaggcc gaagaagaag aaatcaagag ttttcaccag
      481 cgataccgcg agtatctcga aaagattcag agcggtgaac tgtctcaggt ggcctatgtc
      541 taccggctgc tctttcggcg cagtctggtc aaacccctgg gaaccaagga caaagcgttg
      601 ttcgagacag cgcgtcagtt gctcgtatca gagattgtgt atgccaagcg catctctgag
      661 gacgacgctc gcgacatgct cgacaccgcc atggaagaag tcgttttcta gaaaaactct
      721 ccgcgagcct gcatggctcg cggagatcgc ttccgtgaga gaattcagag ggtccctttg
      781 ccccagcgcg ggtgatttcg tacgtccgga ttgaccggat acggcggcag ttgacccgcc
      841 agaaccgcca gcacacactc ggcggccatg gttcccatgc gtgaccgcgt ttcaatcgtg
      901 cctgagccga tgtgtggcag caggacagcg ttgggaacgt tcaacagatc gggatgaacg
      961 atcggttctc gttcgtagac gtcgagaccg gccgcaaaga tcactttttc ccgtagggcg
     1021 cgtgccagtg cggcctcgtc gacgaccgga ccacgcgagg tgttgatcag cacggcggta
     1081 cgtttcatcc gctgcagttg attccacccg atcaggtgcg tggtttctgc cgtcagcgga
     1141 cagtgcagag aaagcacgtc gctctcggcc agcagcgcct caaaagagac cgccggccag
     1201 ggcaggccgg gcttggcccc ggagcgtgtg tgatacagaa ttctcatgcc gaatgcttcc
     1261 gcgcgtctgg ccacggcttc accaatgcgt cccatgccca gaatccccag attcttgcct
     1321 gcgagatcct gcccgagcag gtagatcggt ttccagccgg tgaactcgcc gcgccgcaga
     1381 gagtcgagcg cttcagggac ccgccgcagg gcgtcgagaa tcagaatcat cgtcacatcg
     1441 gcagtggcgt cggtcaaaac atggggggta ttggaaacgg caataccggc agccgtggcc
     1501 gcggcaatat cgatgttgtt gaacccgacg gcgtagttgg ccagcacgcg taaacggggg
     1561 cactggcgca gatgctcggc ggagatcggg tccgacagca ggctgatcgc ggcatctgac
     1621 tgggagagtg ctgcgtaaaa ctcgctctgg gggcggtcgt cggcgagaac gcgcacccga
     1681 tgcccggctt gttccagtcg accgatgcct tcacccggaa acggtcgcga aatgagaatg
     1741 tccatgatcg gctcctgaag atgcgggaat ccgcaatttt cagcgttata ccaccaatcg
     1801 cgatccctgg ctatgaagaa gtctgtcccg agggggtctg aaaagaacgg tggaaatttc
     1861 tcttcagcag gaagcgcttg atgccgaagg ttttactgga tcttcctttt atcatgatgc
     1921 gaaaaccatt gatgtttctg tctctggtgg tgctctggct tctcgccggg ggacgcacct
     1981 acgctccgaa cggtggtctc aaacctccgt ccggggagat accgcgcccg ccatccaggg
     2041 gacgcagcgg cgaagggctg ggcatgcagt atctctcgaa accggaacgc acctacggtt
     2101 actggatggc gcgtggtcgc gagtatctgt cgcgtgccga acacgaacag gcggtgctgg
     2161 cgttccgtaa agctctgaaa gaacgcccgg tgtcagaaga tgcacacttt cttgccggaa
     2221 tcgcctacga gcgacgtggg cgtgaatgtc tgcccggaga cgccaccaac tgggacgaac
     2281 tggccgaaca ggaataccgt gccgccatag ctctgtctga ctatctcccc gctcggtaca
     2341 acct
//
LOCUS       DAORHD010000816         2341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_55947_length_2341_cov_2.982502, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000816 DAORHD010000000
VERSION     DAORHD010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55947_length_2341_cov_2.982502"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="PKO06_15785"
     CDS             <1..930
                     /locus_tag="PKO06_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71164.1"
                     /translation="TPCVAFAPGSREPWTNVGAQRLHDENPNLVSELSRATEEGNLQS
                     GRVFDLTEVGEVSRYLFEPVVTASGTTVWMGWNVQNTLFSSGMELTQMIARKLTHEIR
                     NPLQPIQIIADQWQETHPEFAGDAEIVRTQIDRILKVIEHMRRLLDVRPLQIETLELN
                     GLIEKIVERLRREFSTHVSWSFYRMSPNILIAGEAERLSRTLESLMRNSLEAMPEGGC
                     LQIATGTSGSKQEGGLRAYVEISDSGAGMSTESMGHLFKPFFSTKKDGVGLGLVIARR
                     TIEMHRGQLKFASQEGIGTTATVALPLHETREE"
     gene            937..1593
                     /locus_tag="PKO06_15790"
     CDS             937..1593
                     /locus_tag="PKO06_15790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71165.1"
                     /translation="MNVLLLDDDAAVRDILSRGLARFKHEVRAVASLEEARDALKTFK
                     PDCAVLDMVLGTDLGVDLIPDLKLIDTQMPIIFITGNPDHAVFHDPALTDVIFSMLEK
                     PVSLGRLKTIMEWAAEKRRESERPTSDQLRAIQEAEIDELKELVKTAGWKKIAAYVAF
                     VVLLLSLLFVCFDAYRNYSIDVAAGRSYIDSFMDRVEGYLKRDEMRELMRAKDQRKLK
                     "
     gene            1640..>2341
                     /locus_tag="PKO06_15795"
     CDS             1640..>2341
                     /locus_tag="PKO06_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71166.1"
                     /translation="MFPFWDIVSVAFLGGILLLKRVRDRVIPADHVQLPISAMPATAA
                     EPPDSAESRQDRAVHGTTPCHNRAVNSQAEQEPESCSTEPSDLSVCSPTRSYTWLAEQ
                     LSAIGTEPSRESFDVVRQVEAPAEESAKIPAETAVSEQMPIIAAATGMTEAPDEVSVA
                     EVAAAVDISPAPGAADLGAAQTAETSVPAEERLRKSDQQTAVESLEEEIRRLEELVAA
                     GDEELEQLGNTALLKQ"
BASE COUNT          514 a          626 c          728 g          473 t
ORIGIN      
        1 accccttgtg tggcgtttgc gccaggttcg cgagaaccgt ggacaaacgt gggagcccag
       61 cgcctgcacg atgaaaaccc gaatctggtc tctgaattgt ccagggcgac cgaagaggga
      121 aatctgcaat cggggcgtgt tttcgacctg accgaagtcg gtgaggtcag ccggtatctg
      181 ttcgagccgg tggtcactgc atcaggaacc acggtctgga tgggctggaa cgtgcaaaac
      241 acgttgttca gcagtggcat ggaactgacg cagatgatag cgcgcaaact gacgcacgag
      301 attcgcaatc cgttacagcc gatccagatc atcgccgatc agtggcagga gacacacccg
      361 gagttcgcgg gtgacgcgga gatcgttcgc acgcagatcg accgcattct gaaggtcatc
      421 gagcacatgc gccgcctgct cgacgtacga cccctgcaga tcgaaaccct tgaactgaac
      481 gggctgatcg agaagattgt cgaacggttg cgtcgcgagt tttcgacgca tgtgtcttgg
      541 agtttctatc gcatgagccc caacattctg atcgccggtg aagcagagcg tctgtctcga
      601 acgctcgaga gtctcatgcg caacagcctc gaggcgatgc ccgagggcgg gtgtctgcag
      661 atcgccacgg gtacaagtgg ctcgaagcag gaaggtggtt tgcgtgcgta tgtcgaaatc
      721 tcggattctg gggcgggaat gtcgacggag tcgatggggc acctgttcaa gccgtttttc
      781 tcgaccaaga aggacggggt cggacttggc ctggtcattg ccaggcgaac catcgaaatg
      841 catcgtggac agttgaagtt cgcctcacaa gaggggatcg gcaccaccgc gacggtggcg
      901 ttgccactgc atgaaacgcg cgaggagtaa gcggacatga atgtgctgct gcttgacgac
      961 gatgccgcgg ttcgtgacat tctgtctcgt ggtctggccc gcttcaagca cgaagtacgt
     1021 gccgtagcct cgctcgaaga ggcacgcgat gcgctcaaga cgttcaaacc cgactgcgcg
     1081 gttctggata tggtgctggg caccgatctc ggagtcgatc tgatccctga tttgaagctg
     1141 atcgataccc agatgccgat tatcttcatc accggcaacc cggaccacgc cgtgtttcac
     1201 gacccggcac tgacggacgt gatcttctcg atgctggaaa agccggtttc cctcggacga
     1261 ttgaaaacca tcatggagtg ggccgcggag aaacgccgtg agagcgaacg tccgacttct
     1321 gatcaactga gagccattca ggaagcggaa atcgacgagc tgaaagaact ggtgaaaacc
     1381 gcggggtgga agaagatcgc cgcgtatgtc gcctttgtgg tgctcctgct gtcgctgtta
     1441 ttcgtttgct ttgatgccta tcgcaactac tcgatcgatg tcgcggccgg tcgttcgtac
     1501 atcgattcgt tcatggatcg ggtcgaaggg tatctgaaac gcgacgaaat gcgcgaactg
     1561 atgcgcgcca aggatcaacg caagctcaag tagaaaaagg gcaaagaaac ccctgagttg
     1621 tgccgataac tcaggtgata tgtttccttt ctgggatatc gtttctgttg catttcttgg
     1681 cggaatcctg cttctgaaaa gggtccgcga tcgggttatt cccgccgatc acgtgcagct
     1741 gccgatttcg gcgatgccgg ccacggctgc agagccacct gacagcgctg aaagcagaca
     1801 ggaccgtgcg gtccacggca cgaccccctg ccacaatcga gcggtgaact cccaggcaga
     1861 gcaggagccg gaatcctgct cgaccgaacc atcggatctg agtgtctgca gcccgactcg
     1921 ctcttatacg tggctggctg aacagttgtc ggcgattggc acggaacctt cccgggagtc
     1981 ctttgatgtg gtccgtcagg tggaagcacc ggctgaggaa tcggcgaaga ttcccgctga
     2041 aaccgcggtt tctgagcaga tgccgattat cgccgcagcg accggtatga ctgaagctcc
     2101 cgatgaagtg tctgtcgccg aggtggcggc agcagtcgat atttctcctg ctcccggggc
     2161 agcagacctt ggcgcagctc agacggcgga aacgtctgtg cccgcggaag agcggctgcg
     2221 caagagtgac cagcagactg cagtcgaatc gctggaagag gagattcgac gactcgaaga
     2281 actggtcgcc gcgggagatg aagaattgga gcagctggga aacactgcgt tgctgaaaca
     2341 g
//
LOCUS       DAORHD010000817         2338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56050_length_2338_cov_3.781866, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000817 DAORHD010000000
VERSION     DAORHD010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56050_length_2338_cov_3.781866"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PKO06_15800"
     CDS             <1..1806
                     /locus_tag="PKO06_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71167.1"
                     /translation="PIIPVMRTSVTLPTVVPANAVRGNLVNGIRPLAGVEETALTGAT
                     VTVRLSNGQTVEMTEEPDQPGTYRCQVRSLVGVTTILIEARKGELVIQNLITDPNQID
                     QTEQSPLVTSYLTTTFAQVALAFAQREKGANVVPDLSSLMQQAQNLQLQLTTLRNEIL
                     AESQDYTPQLKFVANALKMANAALENTVQTILEKLKNDPNLQAGGATFAEIMVTALGA
                     IAPPTLPPADEVAEVTRVLNVFLSACYTMMTDRGETQATDAEVASLTEILDDSFLLNG
                     MDKANFVGQFKTGSGEGWGDLLVRQQTIPVLKRFEGAQDWYVGGVRGTLTLQKKDDSG
                     STSEYTVTTDSLMDGWRFNPNTNFDRYESFSLTPFEYLPVALKKVDGKWKIVGNQLPI
                     FMKWLQVNVFGRFGEPATYHSVFARFTGHLKQVKIKGPGLGPNELTLTSTNDFDYAVR
                     DGQGNQVTVPPEGTLSNLPILPGQSYEVTLTDRNDLVKTLWFKVPSYSDVPLQGKVEK
                     NTSGDVVISWQKPVDQQFARYTVQVQFTDTQGATRYWSNFISDINTESTTFPKAGTIW
                     GAAYTADLTKPVLVKVDAESLGGVNHNVFGTFTLN"
     gene            2033..>2338
                     /locus_tag="PKO06_15805"
     CDS             2033..>2338
                     /locus_tag="PKO06_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71168.1"
                     /translation="MTRIVMSRLLGLSVGLFLSIVSPVDACTPVPMAFTMMGNILSGP
                     LMLQMLGAVALVTALKVGLFWRANRGEISFFRLLGAIVAANVASTLYFLIAVIMMEAT
                     "
BASE COUNT          507 a          641 c          691 g          499 t
ORIGIN      
        1 ccgatcatcc cggtgatgcg gaccagcgtg acgctgccga cggtggtgcc ggccaacgcg
       61 gtgcgcggaa acctcgtgaa tggtattcgt ccgctcgcgg gcgtcgaaga gacggcactg
      121 accggtgcga cggtgacggt gcggttgtcg aatgggcaaa cggtcgaaat gaccgaagaa
      181 cctgatcagc ctggcaccta tcgctgtcag gtgcggtcgt tggtcggggt gacgacgatt
      241 ctcatcgagg cgcgcaaagg ggaactcgtg atccagaacc tgatcaccga cccaaaccag
      301 atcgatcaga cggaacagtc gcccctggtg acgagttatc tgacgacgac gtttgctcag
      361 gtggcgttgg catttgcgca aagggaaaaa ggcgcgaacg tcgttcctga cctctcgagt
      421 ctgatgcagc aggctcagaa cctgcagttg cagctgacga cgctgcgcaa cgaaatactc
      481 gccgagagcc aggactatac gccgcagctg aagtttgtcg cgaacgcctt gaaaatggcc
      541 aatgcggcac ttgagaacac cgttcagacc attctcgaga aactgaagaa cgatccgaat
      601 ctgcaagcgg gtggagcaac gtttgccgag atcatggtga ccgccctcgg agctatcgca
      661 ccgccgacac tgcctccggc tgatgaagtg gccgaagtca cgcgggttct gaacgtcttt
      721 ctttctgcct gttacaccat gatgaccgat cgtggcgaga ctcaggcaac cgacgccgag
      781 gtggcatcgc tgacagagat tctcgacgat tccttcctgt tgaacggaat ggacaaagcc
      841 aattttgttg gccagttcaa gaccggcagc ggcgaagggt ggggtgacct gttggttcga
      901 cagcaaacga ttccggttct caagcgcttc gagggtgcgc aagactggta cgttggcggt
      961 gtgcgcggaa ccttaaccct gcagaaaaaa gacgatagcg gcagcacctc tgaatacacg
     1021 gtgaccacgg acagcctgat ggatggctgg cgattcaatc caaacaccaa cttcgatcgg
     1081 tatgaatcgt tctcgttgac gcccttcgaa tacctgccgg tggcactgaa gaaagtcgac
     1141 ggaaagtgga agatcgtcgg aaatcagctg ccgatcttca tgaagtggct gcaggtgaac
     1201 gtgttcggca ggttcggcga gcctgcaacc tatcacagcg tgtttgcgcg gttcacgggt
     1261 catctcaagc aggtgaagat caaaggcccc ggactcgggc ccaacgaact cacgttgacc
     1321 tcaaccaatg actttgatta cgcggtccgc gacggccagg gaaaccaggt cacggttccg
     1381 ccggaaggca cgttatcgaa tctgcccatt ctcccgggcc agagctacga agtcaccttg
     1441 accgacagga acgatctcgt gaaaaccctg tggttcaagg ttccgagcta ttccgatgtc
     1501 ccattacagg gaaaggtcga gaagaatacg agtggcgatg tcgtcatctc ctggcagaaa
     1561 cccgttgatc agcagttcgc gcgctacacc gtgcaggtgc agttcaccga tacccagggt
     1621 gccactcgat actggagtaa cttcatctcg gacatcaata ccgagtcgac gacctttccg
     1681 aaagccggaa caatctgggg agccgcctat accgctgatc tgaccaagcc ggtgttggtg
     1741 aaggtcgacg ccgagagctt gggcggtgtc aatcacaacg tttttggaac ctttacgttg
     1801 aactgacctt ctcgctcgtc cgttgtgtct cgaaccccgg tcgaaagacc ggggttcgat
     1861 cttgcagcga ggatttcagc gcggaccgac tcggacattc gtggttcgta catagcgctc
     1921 cgcaggtgaa cgattcggct cctgagctcc aatcccgtcc tgcgcggaac aggaatcatc
     1981 tgttccggta acacttgccc gaaaaccggg gttcttgtta cgcttgatgt tcatgactcg
     2041 aattgtgatg tcgcgactcc tcggtttgag tgtcgggctc ttcctgtcga ttgtttcgcc
     2101 ggtcgatgcc tgtacgcccg tacccatggc tttcaccatg atgggcaata tcctgtcggg
     2161 tccgctgatg ctgcagatgc tgggggcggt tgcgctggtc acggcattga aagtcgggtt
     2221 gttctggcgc gccaatcgtg gcgagatcag cttctttcgc ttactggggg cgattgttgc
     2281 cgccaatgtg gcttcgacgc tctacttcct gattgccgtg atcatgatgg aagccacg
//
LOCUS       DAORHD010000818         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56088_length_2337_cov_3.906223, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000818 DAORHD010000000
VERSION     DAORHD010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56088_length_2337_cov_3.906223"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1525)
                     /locus_tag="PKO06_15810"
     CDS             complement(179..1525)
                     /locus_tag="PKO06_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71169.1"
                     /translation="MRKLNIVLVLALGAFLMGGDFLAGQTATTGTAATTDQTATTIPN
                     QPMTMDVPGALPASFRGGNTDDGPVNVLEDGNYKIQEHSVLYNDTTSGMGQTAQGTEF
                     TSPPTINWTTERETADGATQNMGNVNNNQASMNASFFQEPGTYRIHNGGARQVSGGAQ
                     DTSSSGSTGSTGATGSTGATGATGSTGSTGTAATGATGATGATGATGATGAAQDQRVT
                     ANQTLTVVSHDCTAPCIWGVIQEGAGTTELAANESQLKQDLREQMESVKGSFAESSEL
                     SGSLKEASLLAVTEFPKNAEPKVKAAAVFVKGPLFDENGKKLGTTPAGAVPMKVADEE
                     KQTRVAQIGASTVKGVFVRRNVPFLAAAQISDNGTYDSNGAVCRIENKTTGAEVEKAE
                     GAYLFRVPNYPREQYADQPEYEFVMEGADAAGNKSQVRVPLFVVNTQASFEGGKAE"
     gene            2123..>2337
                     /locus_tag="PKO06_15815"
     CDS             2123..>2337
                     /locus_tag="PKO06_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV71170.1"
                     /translation="MRIGIPIDNGILCPHFGRCDEFALVDVDETRQIIRQIQITHAPE
                     HEPGVRPRWLIQNGVKIAIVGGIGERAR"
BASE COUNT          486 a          673 c          643 g          535 t
ORIGIN      
        1 gcacagctgg tactgggctg atcgtcactc cctcgagcca cggagtcttg gagggatcga
       61 tatcagttgc cggaccggac gcattacccg tttcaaggtt ctgcgcctgc aggcccgcag
      121 gcccgcaggc gacggccagg aaaagcacca gagcaagacg aagggccttg ttcaccactt
      181 actccgcctt tccgccttca aacgaggcct gagtattcac cacgaacagc ggtacgcgca
      241 cctggctctt gttgccggct gcatccgcgc cttccatgac gaattcgtat tcgggctgat
      301 cagcatactg ctcgcgggga tagttcggaa cgcggaacag atacgcgcct tccgcctttt
      361 ccacttccgc gccggtggtc ttgttttcaa tgcggcacac cgcaccgttg ctgtcatagg
      421 tgccattgtc gctgatctga gcggcggcca ggaacgggac attgcggcgg acaaagacgc
      481 ccttcacagt cgaagccccg atctgggcca cgcgggtctg cttttcctcg tcggcaacct
      541 tcatcggaac ggcgccagcg ggagtcgtgc caagtttctt gccgttttcg tcgaacaacg
      601 gacccttcac gaaaactgcc gcagccttca ctttcggttc ggcgttcttg gggaattcag
      661 tgacggccag gagagaagct tctttcagag atccgctcag ttcagaactc tcggcgaagc
      721 tccccttcac ggattccatc tgttcgcgca gatcctgttt cagctgcgat tcgttggcgg
      781 ccagctcggt ggtaccagcg ccttcctgaa tgacgcccca gatacacgga gcagtacagt
      841 cgtgggaaac aacagtcaga gtctggttcg cggtcacgcg ctggtcttga gcagcgcccg
      901 tagcgcccgt ggcacctgtc gcgccggtag caccagtcgc gccagtggca gccgtacccg
      961 ttgaaccggt tgaaccggta gccccggttg cgccagtcga gccagtggct cctgtgcttc
     1021 cggtcgaacc ggaagaggag gtatcctggg ctccgccgga gacctggcgt gcaccaccgt
     1081 tgtgaatgcg gtaggttccg ggttcctgga agaagctggc attcatcgaa gcctggttat
     1141 tgttcacatt gcccatgttc tgggtcgctc cgtcagcggt ttcccgttcg gtcgtccagt
     1201 tgatggtcgg aggtgaggtg aactcggttc cctgggcggt ctggcccatg ccggaagtcg
     1261 tatcattgta cagaaccgaa tgttcctgaa tcttgtaatt gccgtcttca agcacgttca
     1321 ccggaccgtc atcggtattg ccaccacgga agctggccgg cagagcaccg ggaacatcca
     1381 tggtcatggg ctggttcgga atggtcgtcg ccgtttgatc agtggtcgct gctgttcccg
     1441 tggtcgccgt ctgtccggcg agaaagtcgc cacccatcaa aaaagcgcca agcgcaagaa
     1501 caagcacaat gttcagtttc ctcatacgca ttcctcctga taatttgtgt tcgagactgt
     1561 catccaaccg ttcaagctta cgactctcaa ccgaatcaag atcacgagaa gattcgatta
     1621 cgtgacattt tgtcgagggg cttttcgttg tcttgcggaa aatcatcgtt ctttgacact
     1681 gctgtacact cctagcaaga atgataccga tgggctccag caacctcaaa cgcgtgccag
     1741 ggcaatggtt ttcaccctgt actactcgat tcgcccgcga aaggttacaa aaaagaacaa
     1801 ggaagtgctg tttcagatat tgcatttttg caaggacacg atagtctctg gacgcgactt
     1861 acctccataa aaagctttgt gacagagatt gatcgattgt attgcagata tgcaactatt
     1921 tcgtgagata ttgcaatatt gcactcatcg agaaaacacg ttctggcgac ttgcagcatg
     1981 gtatcacgtt tttcaaactt ggcacaaata atgcgaactt ggacaatgcc gggccgacaa
     2041 ccaacgtccg tctgttcagc aacccggctc cgggcgaaga gagatcttcg cccatttttt
     2101 ccgcccacag tacggaggcc atgtgcgcat cggtattccc atcgacaacg gtatcctctg
     2161 tccgcatttc ggacgctgtg acgagttcgc tctcgtcgac gtggatgaaa ctcgtcagat
     2221 catccgtcag atccagatca cgcacgctcc ggaacacgaa ccgggagtgc gcccgcgatg
     2281 gctgatccag aatggagtca agatcgcgat cgtcggcgga attggggagc gtgcccg
//
LOCUS       DAORHD010000819         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56271_length_2332_cov_4.645586, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000819 DAORHD010000000
VERSION     DAORHD010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56271_length_2332_cov_4.645586"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="PKO06_15820"
     CDS             complement(<1..1279)
                     /locus_tag="PKO06_15820"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV71171.1"
                     /translation="MILRDVTVSRAALVFTFLTGLLVQMVCAAEFIFLPVPQSPLITL
                     RVMIKTGPMHDPSGKEGLAWLMSSVVARGGTGTLSRADIIRRLFPMAADIRCSGGRET
                     LVFDGTVHRDHLKAYYAVFKELVLQPAFDEQDLRRERDAAINWLTNGLRSTEDESLGK
                     EVLELMLYRNHPYQCFGTVDGLLAIDRRDLIEFHRQALSRRKVWVGLAGGYDLGFAEQ
                     LKRDFALVGSDKPEPLLPSPVMPSGLEVTLVQKKCDACAISLGFPVSYTRADPDFFAL
                     MVANSFLGEHRMFIGRLQKTLRMARGMNYGNYSYLDYFLQGDGRFAQCGVPRRQQFFS
                     IWIRPVKPEQAHFALRAALRELRQLVERGMTVDEVEDYKRFVLANSRLWAQTVERRLA
                     YRMDSDFYGRTDFLRDIQRRVARLTAREVNAAIR"
     gene            complement(1276..>2332)
                     /locus_tag="PKO06_15825"
     CDS             complement(1276..>2332)
                     /locus_tag="PKO06_15825"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV71172.1"
                     /translation="LMLMGAESNAFTSDDCTVFHLVGSADALERMMEIESDRFRHLAY
                     AEEDLRTEATVILGEYQKDASNPYTKLEETLRKLAFEKHTYRHTVIGLLEDIRSMPDK
                     YDYSRKFYARGYSPSRCSLVLVGDLNFSRLKSMLDRRYGSWTESAEPPVAPMPEPAPT
                     IERTAHLDWPHETLPYIYMGYRVPAFSDRDADIPALEVLASLLFSASSPLYQKLVLDQ
                     QKVEYLGGSYDRHRDPYLFTIMLRPKRRDEIEGVKREVEAALAEAARVPPSLERLEAV
                     KSNLRYAFAQQLKTPLGTAFTLSEFLWLTGDPSSLSRHVQALSRVGPRDVMRVADRYF
                     HAENRSVVTLSAAEGKR"
BASE COUNT          563 a          672 c          644 g          453 t
ORIGIN      
        1 ggcgaatggc ggcgttcact tctcgcgcgg tcaggcgggc cactcgccgc tgaatatccc
       61 gcaagaagtc ggttcgacca tagaagtctg aatccatacg gtaggcaagt cgacgctcga
      121 cggtctgcgc ccagagacgg gaattcgcca ggacaaatcg cttgtagtcc tcaacctcat
      181 cgacagtcat acctcgttca acgagctgtc gcagttcgcg cagcgctgcc cgcagagcga
      241 agtgtgcctg ttcaggcttt accggtctga tccagatgct gaaaaactgc tgtcgacgag
      301 gaaccccaca ctgcgcaaat cgaccatcac cctgcagaaa gtagtcgaga taggaatagt
      361 tgccatagtt cataccccga gccatgcgca aggttttctg cagacggccg atgaacatgc
      421 gatgctcgcc gagaaaggag ttcgccacca tcagcgcgaa aaagtctgga tcagcccgag
      481 tgtaagacac cgggaacccg agagaaatcg cgcaggcatc gcactttttc tgcaccagcg
      541 ttacttcaag cccggacggc atgacaggag agggaaggag tggctcaggt ttgtccgagc
      601 caacgagggc aaaatctcgc ttcagttgtt cagcaaagcc cagatcatat ccaccagcaa
      661 gccccaccca cacctttcga cgcgacagcg cctgacggtg gaactcgatc agatcacggc
      721 gatcgatggc cagtaacccg tcgaccgtac cgaaacactg atacgggtga ttccgataca
      781 acatcagctc cagcacttct tttcccagac tttcatcttc ggtgctacgc aacccgttcg
      841 tcagccagtt gatggctgca tcgcgctcgc gccgaagatc ctgttcatca aaagccggct
      901 gcagcacgag ctccttgaac accgcatagt aggccttcag atgatcacga tgaacggtcc
      961 catcgaacac cagagtttcg cgaccgcctg agcagcgaat gtcggcagcc atgggaaaca
     1021 aacgccgaat gatgtcggca cgcgacaacg tcccagttcc tccacgagcg accacgcttg
     1081 acatcagcca agccaggcct tcttttccag acgggtcatg catgggccct gtcttgatca
     1141 tgactcgaag agtgatgagc ggcgactgtg gaaccggaag gaagatgaac tcagcggcac
     1201 ataccatttg caccagaaga ccggtcagaa aagtgaacac cagagcggcc cggctgacgg
     1261 tcacgtctcg caggatcatc gtttcccctc cgccgcgctc agggtaacca ccgagcggtt
     1321 ttcggcatga aagtaccggt ccgccacgcg catgacatcg cgggggccaa cgcgtgacag
     1381 tgcctgaaca tgtcgagaca gcgacgaggg gtctcccgtc agccagagga attcactcag
     1441 ggtaaacgct gttccgagag gggtcttcaa ctgctgagca aacgcatagc gcagattcga
     1501 tttgactgcc tcgagacgtt cgaggctcgg tggaacccga gccgcttcgg ccagagccgc
     1561 ctcgacttcg cgcttcaccc cttcgatctc atcgcgtctt ttcggtcgca gcatgatcgt
     1621 aaaaaggtat gggtcgcgat gtcgatcata ggacccaccg agatactcga ccttttgttg
     1681 atccagaacc agtttctgat agagcggaga agacgcggaa aacagcagcg aagccagcac
     1741 ttcgagggcg ggaatgtcag catcgcgatc agaaaaggcg ggaacgcgat accccatgta
     1801 gatgtagggg agtgtctcgt gaggccagtc aagatgcgca gtccgctcaa tcgttggcgc
     1861 aggttccggc atcggcgcga cggggggttc cgcactctcg gtccatgaac cataacgacg
     1921 gtcgagcata ctcttcaggc gtgaaaagtt gagatctccg acgagaacga gcgaacaacg
     1981 tgacggcgag tagccgcgcg catagaactt tcgactgtaa tcgtacttgt caggcatcga
     2041 tcgaatatct tccagaagac caatcacggt atgacgatag gtgtgtttct cgaaggcgag
     2101 tttgcgcagg gtctcttcga gcttcgtgta aggattcgat gcgtctttct gatactcgcc
     2161 gagaatgacc gtggcttcgg tacgcagatc ttcctcggca tacgccagat ggcgaaatcg
     2221 atcactttcg atttccatca tgcgttcgag cgcatcggca ctgccgacca gatggaagac
     2281 cgtgcaatca tcagaagtga acgcattcga ttcggcaccc atgagcatca gt
//
LOCUS       DAORHD010000820         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56288_length_2332_cov_2.778217, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000820 DAORHD010000000
VERSION     DAORHD010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56288_length_2332_cov_2.778217"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKO06_15830"
     CDS             <1..519
                     /locus_tag="PKO06_15830"
                     /inference="COORDINATES: protein motif:HMM:NF018053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HNV71173.1"
                     /translation="FPLNGYQMHCTLYMTDYAPEQLETLKQAVQSLAFRLARIPATTT
                     GLYRTDNDWLFVNIERSPALQALSDLVVKKLSPYRAPKSNPPDWIKNNPVKLENFLKY
                     GSPNVFAGFEPHLTLLAKTDAGKLNEVEQAVAAQPELNKPVKGYVAGIGLAIADRNGQ
                     MKDPIAIHPFKR"
     gene            complement(685..1152)
                     /locus_tag="PKO06_15835"
     CDS             complement(685..1152)
                     /locus_tag="PKO06_15835"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV71174.1"
                     /translation="MRARLFLDSSVWLAGLGSPLGGAGWLLFLMEAGEIEVISSEQIL
                     VEVRRNLEDKKVRYASRLETILKHRKPRTLPEFKDTELLRWLYLVPPKDCHVLAGAFK
                     SEADALITLDKEHILTPTVKAAFPLPIMTPGEFLEAFHLETEREDNLRRRKGS"
     gene            complement(1149..1439)
                     /locus_tag="PKO06_15840"
     CDS             complement(1149..1439)
                     /locus_tag="PKO06_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71175.1"
                     /translation="MPRKALEPTTTITVRLPTSMVSALDEEAERLRRETPGLQATRAD
                     ALRYFISMGQARLDMSAFREPRIYDDEQLERFKEQDRIPLKTCNWLKKYYKR"
     gene            complement(1709..>2332)
                     /locus_tag="PKO06_15845"
     CDS             complement(1709..>2332)
                     /locus_tag="PKO06_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71176.1"
                     /translation="SFLGTFVYQHIRQDLLWDNDAIVQDGQTAYIAEPEKAIRDLFHL
                     HRRTVTPEYLDEMRLQKLRALSRCRLRYYAARFHLNFISEAADLMIARLKGTKPRKTS
                     CSGSSNRCRRLGNILRRDFEAGGYSATVRERADKSVVVLEVSFSGVLRETRITPDPRK
                     KLMIKVEIDTRLPRGSKTTVTLSNKYFPLALKHNDLETLLALAKTAA"
BASE COUNT          505 a          650 c          630 g          547 t
ORIGIN      
        1 tttccgctga acgggtatca gatgcactgc accctgtaca tgaccgacta cgcgcccgag
       61 cagctcgaaa ctctgaaaca ggccgtgcag tccctggctt tccggctcgc tcgcattccg
      121 gcgaccacca ccggcttgta tcgcaccgac aacgactggc tgttcgtcaa catcgaacgc
      181 tcaccggctt tgcaggcgct ctctgacctg gtcgtcaaaa agctttcgcc ctatcgcgcg
      241 ccgaaatcga accctcccga ctggatcaag aacaacccgg taaaactcga gaacttcctg
      301 aagtacggca gcccgaatgt cttcgccggc ttcgagccac atctgaccct gttggccaaa
      361 accgacgccg gcaaactgaa cgaagtggaa caggccgtcg ccgctcaacc cgaactgaac
      421 aaaccggtga agggctacgt cgccggcatc ggcctcgcca tcgccgaccg caacggccag
      481 atgaaagatc ccatcgccat ccaccccttc aaacgctgat cgtatagtac aagtcgacaa
      541 cacgccgcgc acgcatcagg cgatgcatgt gcggcgttgt tctattgtct attctccacg
      601 cgaatggttc agttcgctat tctttcacga gaaacggcga gggttttccg tagcaggtga
      661 tctgtcgagc cagtacccgt gaaatcacga ccccttcctc cgtcgcaggt tgtcctctcg
      721 ttcggtttcc agatgaaacg cttcgaggaa ctctcctggc gtcatgattg gtagcgggaa
      781 agcggctttt accgtcggcg tgaggatgtg ttccttatcg agggtgatca gggcatcggc
      841 ttcggatttg aaagccccgg ccagcacatg gcagtctttg ggcggaacca gatacagcca
      901 tctcagaagc tcggtgtcct tgaactctgg cagggtacga ggtttgcggt gtttcagaat
      961 cgtttcaagt ctgctggcat accgaacctt cttgtcctcg agattccgac gcacctcaac
     1021 cagaatctgc tcagacgaaa tcacctcgat ttcaccggct tccatgagaa acaggagcca
     1081 gcccgctccg ccgagaggag atcccagtcc ggccaaccag acggaggaat cgaggaacag
     1141 cctcgctctc atcgcttgta gtattttttc aaccagttgc aggtcttgag aggaattcga
     1201 tcctgctctt tgaagcgctc taattgctcg tcatcgtaga ttcttggttc gcgaaaggca
     1261 gacatatcca gtcgtgcctg ccccatcgag atgaagtacc gcaatgcgtc tgcacgggtg
     1321 gcttgaagac cgggcgtttc acgacgaagc cgctcggctt cttcatcaag cgcgctgacc
     1381 atcgaggttg gcaggcgcac ggtaatagtg gtggtcggtt cgagcgcttt cctgggcatg
     1441 ggttcctccc ggcaggttat tacagataat tatactgtaa tacatatagt gacgaaaaga
     1501 actttttcga caacgatcgt actgtatcgc gctattgtcg aaaatgtttt tccggaatcg
     1561 attcctgttc gagtgccggt agcagagttc ggggaatcat caacgatcag catccctgaa
     1621 gtttggaata ttttggaagt aattccagaa tagtatgtat tattctggat tcgattccag
     1681 tatttcacag tttccgaact cgtccgcgtc aggcagctgt tttcgccagt gcgagcaggg
     1741 tttcgagatc gttgtgcttc agggccagcg gaaagtactt gttcgaaagg gtgactgtgg
     1801 tcttgcttcc acgaggcaaa cgggtgtcaa tctcgacctt gatcatgagt ttctttctcg
     1861 gatccggtgt gatgcgcgtt tcacgcaaca cgccagaaaa ggaaacttcg agaacgacga
     1921 ccgacttatc tgcgcgttct ctgaccgtcg cgctataccc gcccgcctca aaatcgcggc
     1981 gcaggatatt cccgagtctg cggcaacggt tcgacgaacc tgaacagctg gtctttcgcg
     2041 gtttcgttcc tttcaggcgg gctatcatca ggtcggcggc ctcgctgatg aaattgaggt
     2101 ggaaacgtgc ggcgtaatat ctgagtctgc accgcgagag cgctcgaagt ttctgcaggc
     2161 gcatttcgtc gaggtattcg ggcgtcacgg tgcggcggtg caggtgaaac aggtctcgaa
     2221 tcgccttttc gggttcggcg atgtaggccg tctgcccgtc ctgcacgatt gcgtcattgt
     2281 cccagagcag atcctggcga atgtgctggt agacgaaggt gccaaggaaa ga
//
LOCUS       DAORHD010000821         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56422_length_2328_cov_3.880774, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000821 DAORHD010000000
VERSION     DAORHD010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56422_length_2328_cov_3.880774"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PKO06_15850"
     CDS             <1..180
                     /locus_tag="PKO06_15850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71177.1"
                     /translation="VIPPASSTWQIKATPRNQVCTFTGTDEYAGTVFLPTADEPAFRQ
                     WQKTDQSSTTTPSKP"
     gene            complement(274..1812)
                     /locus_tag="PKO06_15855"
     CDS             complement(274..1812)
                     /locus_tag="PKO06_15855"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNV71178.1"
                     /translation="MAAHLGKAFGSLAALTLLSRLSGFVRVAVFAAMYGGGREADVFL
                     SAMLIPELMYRFVSEGLVAGAAVPMFVRSRDSADERQTIFWTQFWGLSAIAIVLTAVL
                     WIPAAWYCRLLVPGFLPDAQAHMAGIWRSMSPYVLFSLQAAVITACLQAAGQFARPAV
                     GPILVNLVIIGGILIVPVDRLELLGLIVTFSGFVQVVWLLWLAARDGIGWRSPWSNQG
                     FRREVFTEFVGRSAPIAGWVVVTPVVPLIERYLLSFQAEGSVSILNYTDKILYLPLGI
                     VSLSLASVVFPRLSEQEPDQAAGTLARVLEALLLVLFPAVLVMHAGAAETAEVIFARG
                     RFAARETLLTAELLSAYAIFLVPASITLLINRLLYASGWYKTTLFIGLAAITAQVAGD
                     TVLVGRLGPVGVGWGAVLAGTLQVTLLLAATARIFSRERCLALLSPVLLAAVAVPLCL
                     PLIRALGSSVRSLFPAGPLGSLLHLTVLWATLQAPLALYWLHRGGYLRARQTPSPPST
                     ETGK"
     gene            complement(1874..2125)
                     /locus_tag="PKO06_15860"
     CDS             complement(1874..2125)
                     /locus_tag="PKO06_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71179.1"
                     /translation="MKFALMALIVFLAIGFVACPVMAGDCDGGSCPIPPDGDDDGHDG
                     CGGCGGHKTADGDCDGGSCPIPPDGDDDDHGGCGGCGGH"
BASE COUNT          546 a          760 c          686 g          336 t
ORIGIN      
        1 gtcattccgc ccgccagttc cacctggcag atcaaagcca ccccgcgcaa tcaggtctgt
       61 accttcaccg gcactgacga atacgccggc acggtctttc tgcccaccgc tgacgaaccc
      121 gcgtttcgcc agtggcagaa gacggatcag agctcgacga cgacaccttc gaagccctga
      181 cgcggccaca cgctcgacct tcggggtcac cgcagcgggt aagtacaagg gagcgccgag
      241 gtcagcgcag atgcgaagag cctgagacga gacttacttc cctgtttctg tcgacggtgg
      301 cgatggggtt tgtctggcgc gcagatagcc gccgcgatgc agccagtaga gggccagtgg
      361 cgcttgcagt gtcgcccaca gaaccgtgag gtgaagcaga ctccccagcg gacctgccgg
      421 aaacagcgac cgcacggaag agcccagagc ccggatcaac ggcaggcaga ggggaacggc
      481 aaccgccgcc agcagtaccg gggagagcag ggcaagacaa cgttcgcgac tgaagatgcg
      541 ggcggtggct gccagcaaca gcgtcacctg aagcgttccg gccaacaccg ccccccaccc
      601 cactcccacc ggtcccagac gaccgacgag caccgtgtct cccgccacct gggccgtgat
      661 cgccgccagt ccgatgaaca acgtggtttt gtaccagcca gatgcgtaga gaagacggtt
      721 aatcagcaga gttatcgatg ccggaaccag aaaaatcgca taggctgaaa gcagttcggc
      781 cgtcagcagg gtttcgcggg cggcgaatcg gccgcgggcg aagatgactt cggcggtttc
      841 ggcggccccg gcgtgcatga ccagcaccgc gggaaacagc accaacagaa gagcctcgag
      901 cacccgcgcc agcgtgcccg cggcctgatc gggttcctgc tcgctcaatc gcggaaagac
      961 aacggaggcg aggctcagag aaacgatgcc cagaggcaga tacaggatct tgtcagtgta
     1021 gttgagaatc gacaccgagc cttccgcctg aaacgacagc agataccgct cgatcagcgg
     1081 aaccaccggc gtcacgacga cccagccggc aatgggggcg gaacgaccga cgaactccgt
     1141 gaacacctcg cgccgaaatc cctggttcga ccagggggat cgccaaccga taccatcacg
     1201 agcggcaagc cacaacagcc agacgacttg cacgaaccca ctgaaggtga cgatcagacc
     1261 gagcagctca agtctatcga ctgggacaat cagaatgccc ccaatgatga ccagattcac
     1321 caggatcggg ccgaccgcgg gtcgagcaaa ctgcccggcg gcctgcagac aggcggtgat
     1381 caccgcggcc tgcagagaga acagcacata cggcgacatc gaccgccaga ttcccgccat
     1441 gtgcgcttgt gcgtcgggca gaaagccggg caccagcagt cggcagtacc aggccgccgg
     1501 gatccagagc acggcggtca gaacaatcgc gatggcgctc aatccccaga actgagtcca
     1561 gaagatcgtc tgtcgttcgt cggcggaatc acgggaacgc acgaacatcg gcacggcagc
     1621 cccggccacc agcccctcgc tgacaaaccg gtacatcagt tcgggaatca gcatggcgga
     1681 gagaaacacg tcggcttcgc gaccaccgcc gtacatggcg gcaaacacgg cgacgcggac
     1741 aaatccgctc agtcgcgaca gcagggtcag tgccgcgagt gagccgaatg cttttccgag
     1801 atgagcagcc atgagaaaga gataagaaaa ccccggccga ccggccgggg ttcgaggttc
     1861 atcgaatcag gactcagtga ccgccgcagc ctccgcagcc gccgtgatca tcatcgtcgc
     1921 cgtccggagg aatcgggcag gaaccgccgt cgcagtcgcc gtcagccgtc ttgtgaccgc
     1981 cgcagccgcc gcaaccatcg tgtccgtcgt catcgccatc cgggggaatc gggcaggaac
     2041 cgccgtcgca atcgccggcc atgaccggac aagccacgaa accgatcgcc aaaaacacaa
     2101 tcagagccat cagggcaaac ttcatactct atacctccga caaccttata tgcgcaggcc
     2161 gttaacccac gcactcttat cgataccgta gcagagtttt tttaccctgt caaacgtacc
     2221 ccagggcgaa ccagggtcat ttaattctcc tccttacgtg ttttccgttc acgtcgctca
     2281 acgattcacc gttacggcag ggagtgcgct cgtcgtctcg agaccgag
//
LOCUS       DAORHD010000822         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56443_length_2328_cov_2.805543, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000822 DAORHD010000000
VERSION     DAORHD010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56443_length_2328_cov_2.805543"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKO06_15865"
     CDS             <1..541
                     /locus_tag="PKO06_15865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71180.1"
                     /translation="DARVRANALTALRRVFQALGRVEEAIVQKMMNCLSDTEHRVRAD
                     AIFNLWELGRIESLDCIECMLQEERPEARASGAWLCGRLKLHQLKDALQHLLRDREWN
                     VRKTAAVALLSFGESGRQMLVSIARTGTPEEQVSAAFALGLDHDAEGIERLFLNSRNG
                     EELSQGATDLLLRFARPVP"
     gene            560..1681
                     /locus_tag="PKO06_15870"
     CDS             560..1681
                     /locus_tag="PKO06_15870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71181.1"
                     /translation="MHQHLESIGFNLIELIGQLDNLGVYITDRNRRILFWNQGAQRIM
                     GYEAEEVVGKACRENVLCHIDRHGRPLCGEDVCPLFHAIRTGKASTVSSFVFGKHRNG
                     KRIPVSVTVSPLRDAQGDVVGGIELFRDATAEWDQLRMAQGIQRRFMPDPLELSRELP
                     LAYHWLPAEMIGGDVVQVQTVAPGIVFGLFVDVSGHGIASALLTGFLWRALEGVRNPD
                     LRPASVLKELARQYQSLNLETHYFSAVCFRYELAARRLILANAGHPLPVVVERPEPAR
                     YLDLTGDLIGLFEMPEFSEQELVMKNARVVIYSDGCVEARDPAGVPFGGERFLRQVEQ
                     AGALPGVAAAKHLVEVAFDYTQAPEPEDDMTVLVIDSPA"
     gene            1764..>2328
                     /locus_tag="PKO06_15875"
     CDS             1764..>2328
                     /locus_tag="PKO06_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNV71182.1"
                     /translation="MSSTDTAQSEIQHKPFVADDQQMPEFTFLPIVVGIVLGIVFGAS
                     SLYLVLKVGMTISASIPVAVISITLFRALSKMFGLRQATILENNIVQTTGSAGESIAF
                     GVGVTMPALMILGYEMDVARVMTVSVLGGLLGVLMMIPLRRGLIVKQHGKLTYPEGTA
                     CAEVLIVGEQGGTNAKTVFTGFGLGFLY"
BASE COUNT          444 a          612 c          713 g          559 t
ORIGIN      
        1 cgatgcccgt gtgcgtgcga acgccttgac ggcgcttcgt cgggtgtttc aggctctggg
       61 ccgagtcgaa gaggcgattg tgcagaagat gatgaactgt ctcagtgata cggaacaccg
      121 ggtgcgggca gatgccatct tcaatctctg ggaacttgga cgcatcgaga gcctggattg
      181 catcgaatgc atgctgcagg aggaacgccc ggaagcgcgg gcttcaggcg cgtggctttg
      241 cgggcgcttg aaactgcatc aactcaagga tgcgctgcaa cacctgttac gcgaccgaga
      301 atggaacgta cggaagaccg cagctgtagc cttactttca tttggcgaaa gcggccgtca
      361 gatgctggtt tccatcgcgc gaaccggtac tcccgaagaa caggtcagtg ccgcgtttgc
      421 gctcgggctt gaccacgatg ccgagggaat tgaacggctg tttctgaact cgcgcaacgg
      481 agaagagtta tcgcaggggg cgaccgatct gctgctcaga ttcgctcgtc ccgttccctg
      541 aaaccatcgg gcgagattca tgcatcagca cctcgagagc atcgggttca acctgatcga
      601 gctcatcgga cagctcgata atcttggcgt ttatatcacg gatcggaatc gccgcatttt
      661 gttctggaat caaggcgctc agcggatcat gggctacgag gcggaagaag tcgtcggaaa
      721 agcctgtcgt gagaatgttc tctgtcatat tgaccgtcat ggacggccgt tatgcggcga
      781 agatgtctgt cctctgttcc acgcgatacg caccggaaaa gccagtacag tttcgagctt
      841 tgtctttggc aagcatcgca acggcaagcg aattcccgtt tcggtcacgg tttcccccct
      901 gcgggatgcc cagggtgacg tggttggtgg catcgaactg ttccgcgatg cgaccgcgga
      961 gtgggatcag ctcaggatgg cacagggcat acagcgaaga ttcatgcctg atcctctcga
     1021 actcagtcgt gagcttccgc ttgcctatca ctggttgcca gcggaaatga tcggtgggga
     1081 tgtcgttcag gtgcagaccg tcgcgcctgg aatcgtcttc gggttgtttg tggacgtttc
     1141 cggacatggt attgcctcag ccctgctgac gggttttctc tggcgtgcgc tcgagggagt
     1201 gcggaatccc gatctgcgtc cggcatccgt gttgaaggaa ctggcacgtc agtaccagtc
     1261 gttgaatctc gaaacccact atttttcagc ggtctgcttt cgctatgaac tcgcggcgcg
     1321 gcgtctcatt ctcgcgaatg ccgggcaccc attgcccgta gtggtcgagc ggcctgaacc
     1381 ggcgcgatat ctcgatctca ccggcgatct gatcgggttg tttgagatgc ctgagttcag
     1441 tgagcaggaa ctggtcatga agaacgcccg agtggtcatt tactcggatg gctgcgtcga
     1501 agctcgtgat ccggctgggg ttccgttcgg gggtgagcgc tttctcagac aggtcgaaca
     1561 ggctggtgct ctgcctggcg tggccgcggc aaaacatctg gtggaggttg ccttcgatta
     1621 cacacaggct cccgaacccg aagatgatat gacggttctg gtcatcgata gcccagcctg
     1681 atcgagaaga ttgccgtttt gtcggcgtga ctttttgtgg taaggttgcc catattctga
     1741 accttgccgc ggagggtctg ttcatgtctt caaccgatac tgcccagtcc gaaatacaac
     1801 ataaaccgtt cgttgctgac gatcagcaga tgccggaatt caccttcctg ccgattgtcg
     1861 taggcattgt tctcggcatc gtcttcggcg cgtcatcgct ctatctggtg ctgaaagtcg
     1921 gcatgacgat cagtgcgtcg attccggtag ccgtcatctc gatcaccctg ttccgggctt
     1981 tatcgaagat gtttggcctg cgccaggcca cgattcttga aaacaatatc gtgcagacga
     2041 ccggctcggc cggcgagtcg attgccttcg gcgtgggcgt gaccatgccg gctctgatga
     2101 ttctcgggta tgaaatggat gtcgcccggg tcatgaccgt ctcggtgctg ggtgggctgc
     2161 tgggtgttct catgatgatt ccgctgcgtc gtggtctgat cgttaaacag catggcaaac
     2221 tgacctatcc cgagggaacg gcctgtgccg aggtgttgat cgtcggcgaa cagggtggca
     2281 cgaatgccaa aacggtcttt acgggttttg gtctcgggtt tctgtaca
//
LOCUS       DAORHD010000823         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56607_length_2324_cov_3.152049, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000823 DAORHD010000000
VERSION     DAORHD010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56607_length_2324_cov_3.152049"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..661)
                     /locus_tag="PKO06_15880"
     CDS             complement(41..661)
                     /locus_tag="PKO06_15880"
                     /inference="COORDINATES: protein motif:HMM:NF019413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HNV71183.1"
                     /translation="MSEPSFRPQDDLEVISCSVVRPELEHLRQSGEFPYPVSYIDSEM
                     HMRPQELGQTIGRRLQTLLRAGKRVLLVYGDCHAAMVDMEQSPRVSRTEALNCVELLI
                     SKQQRREWLERRFFFLLPEWLERWESILLRLPGMTREQCIDLFHREHRGLVYLDTGVI
                     PTPTVNLLQCSETLALLYEILPTGLDHFRSVLHLALLKLHREDART"
     gene            complement(654..1676)
                     /locus_tag="PKO06_15885"
     CDS             complement(654..1676)
                     /locus_tag="PKO06_15885"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNV71184.1"
                     /translation="MNSPERVMAAIAGKPHDRPPFGLVLSLYGAQLIDSRLEDYFRDP
                     DAYTRAQERILDLFHPDILFGPFALALLGEAWGSQLKFQKEQAPNVKRPAAKTVSEFL
                     ALPEPDIEGHPSLKFLRESVRGVVRKTQGRAVVAMPMMSPIDLPALILGLETWIELFL
                     LHPHDVDAVYERTSRFTAKWCQAIMSEGVGCLVMPAPMLSCSLLPLHMIESRSLPLYR
                     EFFSTNSFPTLIHHGGERLLPSLLLAARLPPSVIGFAADARDSLGEVRSLVGPNRVVI
                     GGIEGPQLHAQREDALREQTRLILTDRQDDPHFIFCTTGADVSVHASVDQIRAIADTI
                     EHFRHE"
     gene            complement(1673..2323)
                     /locus_tag="PKO06_15890"
     CDS             complement(1673..2323)
                     /locus_tag="PKO06_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366094.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV71185.1"
                     /translation="MQDLLESINAADYEAAVTQLKLWGSEHGFSRLAEDVLIPLMNRY
                     SELFGAAEEVPLARAYVAAKVTEEGMRLVALSMSPDRQPKLVKGPVVIGNIEDDYHSL
                     GRKIVLTFLQADGWMVHDLGNDVLAETFVDKAVEVGASVIGVSAMMYSTAKNIRSVRT
                     LLDERQLHGKIKLAVGGAVFVMRPELVAEVGGDGTCRSAIGAPALFAELSRKENQA"
BASE COUNT          508 a          654 c          678 g          484 t
ORIGIN      
        1 gtcatgaagg aaaccgtcgg atcaagcatg cgctggtcac tcatgttctg gcgtcttccc
       61 ggtgaagctt cagcagcgcc aggtgcagca cgctgcgaaa atgatcgaga cctgtcggta
      121 gaatttcata gagcagtgcc agtgtttcgg agcactgcag cagattgacg gttggcgtcg
      181 gtatgacgcc ggtgtccaga tagacaaggc cccgatgttc ccggtggaag agatcgatgc
      241 attgttcccg ggtcattccg ggcaatcgca agagaatact ctcccagcgt tcgagccact
      301 caggaagcag aaagaagaaa cgccgctcaa gccactcccg tcgctgctgc ttgctgatca
      361 gcaactcgac gcagttgagt gcttctgttc gcgagacgcg tggcgattgt tccatgtcta
      421 ccatcgcggc gtggcagtct ccatagacca acaggacgcg tttgcccgcc cgcagcaggg
      481 tttgtaaacg ccttccgatt gtctgtccca gttcttgcgg tctcatatgc atctcggaat
      541 cgatataaga gacagggtag gggaattctc ccgactggcg caggtgttcg agctcaggtc
      601 gaaccacgga gcagctgatg acttcgaggt catcctgggg gcggaacgag ggctcactca
      661 tgtcgaaaat gctcaatggt gtcggcgatc gcacgaatct ggtctaccga agcatgcacc
      721 gaaacatctg ctccagttgt gcagaagatg aaatgggggt cgtcctggcg gtcggtcaag
      781 atcagacgcg tctgctcgcg aagcgcatcc tccctttgcg cgtgcagttg cgggccttca
      841 ataccaccga tgaccactcg gttgggtccg acgagactgc gcacttcacc gagtgagtca
      901 cgagcgtcgg ccgcgaagcc gatcaccgat ggcggcaatc gcgctgccag cagtaacgat
      961 ggcagcaatc gctcaccacc atgatgaatc agcgtcggaa aggagttcgt ggagaagaac
     1021 tcgcgataca agggaagcga gcgggactcg atcatgtgga gtggcaacag ggaacaactc
     1081 agcatcggag cgggcatgac gagacagcct accccctcgg acataattgc ctgacaccac
     1141 ttcgctgtga agcgactcgt acgttcatag acggcatcga catcgtgcgg atgcagcaga
     1201 aacagttcga tccaggtttc gagccccagg atcagggccg gaagatcgat cgggctcatc
     1261 atcggcatgg caacaacagc ccgaccctga gttttgcgga cgactccccg cacggattcc
     1321 cgtaagaact tcaaactcgg atgtccttcg atatccggtt cgggcagagc cagaaactca
     1381 gaaacggttt tcgccgcggg cctcttgacg ttcggggcct gctccttctg aaacttcagt
     1441 tggctgcccc aggcttctcc gagcaacgcg agagcaaacg ggccgaagag aatgtccggg
     1501 tggaagaggt caagaatacg ctcctgggca cgtgtgtagg catcaggatc acgaaagtag
     1561 tcttcgagtc tgctgtcaat caattgagcg ccatacagcg agagtacaag tccgaatggg
     1621 gggcggtcgt gcggtttccc ggcgatggcc gccatcacgc gttctggact gttcatgcct
     1681 ggttctcttt tctggagagt tcggcgaaga gcgctggggc accaatggcg ctgcggcagg
     1741 tgccgtctcc cccgacttca gcgacgagtt cagggcgcat gacaaacacg gctcctccga
     1801 ccgcaagctt gattttgccg tgtaactgtc gttcatccag cagtgttcgc accgagcgta
     1861 tgttcttcgc ggtcgaatac atcatggccg atacgccgat tactgaggcc cctacctcga
     1921 ccgctttgtc gacgaaggtt tctgccagaa cgtcgttgcc aagatcgtgc accatccacc
     1981 catcggcttg cagaaacgtc agtacgatct ttcggcccag cgaatgataa tcgtcttcga
     2041 tgttgccgat gacgacgggg cccttgacca gcttcggctg ccgatcaggg ctcattgaga
     2101 gcgcaaccag gcgcatgcct tcttcagtga ctttggcggc gacataggca cgagccagtg
     2161 gaacctcttc tgctgccccg aagagttctg agtagcgatt catcaacgga atcagaacgt
     2221 cttccgcgag tcgactgaat ccgtgctcgc tgccccagag tttcagttgc gtgaccgccg
     2281 cttcgtaatc ggcggcatta atggattcaa gaagatcctg gatg
//
LOCUS       DAORHD010000824         2323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56635_length_2323_cov_4.523810, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000824 DAORHD010000000
VERSION     DAORHD010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56635_length_2323_cov_4.523810"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..>2323
                     /locus_tag="PKO06_15895"
     CDS             130..>2323
                     /locus_tag="PKO06_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71186.1"
                     /translation="MTMLLMVRSRCGMVVLASLLLFGCLSTTNPLCADLAGLQTEKKA
                     CLDELAVTREAIELAKEAVADAARIKAGIERTLGIYLSARDEHARVLTQIRSRLREID
                     IQLAKLRSPNDLADAWNAYKNNRSREQFIHLWKTARGVPPDHATLEDYDLNARILHKE
                     ITNKVVIPVGLCLVKIGGLIKQINELAFNGVGICHPRDADGALEAVDELLGTMQKHFQ
                     KFETRFGMKDDKLLAAGVVVELSKNFQDLYDPQELLPEKLKNWAQELGDNKYVNWLPQ
                     RAIGKLQEEAERMADEAYEQANIDLYGTADRAGEYEKYEKLRDEFSKAADESEKLLAA
                     AQQPVDEFSKRVAETRERFDRLQQTFDKLVQRQAELEKRHLDLLADIFSETKRELAKP
                     IPGETVAVAKPELVFESVPDEASRQIARYDSGTLAADEVTSRLLGIPMELRFGSETKV
                     AQVMRSKETIAETDGSGRQTMKFLSRISLDSPRIVYNYDKVRFSESTGCVSLQSGGRR
                     ISGRKPGRAQVKLTVAGAGAITYDAYGNQRLETQEYLSQQAIQVVSLKDLEARFQDTS
                     TRTPVERIDLATPEGGSNSSPQLQFFARIDWGHEITSRVLDLSHLSIRVIGDPCVTVI
                     RRQNSIILETTNRPGACELEVGTIGQARFTRRIKVTNSIIRGVVRMPDGMQHRAGTLQ
                     KPLSLPIAVPAELAVVIQGPASPHDFLVEWECERHGNAIKSGTGGVEAR"
BASE COUNT          569 a          577 c          677 g          500 t
ORIGIN      
        1 ttcgtcactt ttctgtataa tctgacccga ttcgattggt gaagggctgt ctcggccaga
       61 ggataccagg ctgaggagct aagacgtcga taaaagaata ctgtgtctgg atgctggagg
      121 aatgcccgta tgacaatgtt gctgatggtg cgttcacgtt gcggcatggt ggtgctcgca
      181 agtctgttgc tattcgggtg cctttcgaca acgaaccctc tttgtgctga tctagcggga
      241 ctgcagacag agaagaaagc ttgtctcgac gaacttgcgg tgacgagaga ggcgatcgaa
      301 ctggcgaagg aggctgttgc cgacgcagcc agaatcaagg ctgggatcga aaggacactg
      361 ggtatttatc tgtccgcccg tgacgaacat gcccgagtgc tgactcagat tcggtcccga
      421 ttgcgcgaaa ttgatattca gctggcaaaa ttgcggtcgc ccaatgattt ggccgatgcc
      481 tggaacgcct acaagaacaa tcgcagcaga gaacaattca tacacctctg gaaaactgcc
      541 cgcggcgttc cgcctgacca tgcaacactc gaagactatg atctgaacgc gcgcattctg
      601 cacaaggaga ttacgaataa ggttgtcatt ccggtcggct tgtgtctggt caagattggg
      661 ggtctgatca agcaaatcaa cgaactggcc ttcaacgggg tcggcatttg ccatccgcga
      721 gacgcggatg gagcccttga ggctgtcgac gagctgctgg ggacaatgca gaagcatttt
      781 cagaagttcg aaactcgctt tggcatgaag gacgacaaac ttttggctgc cggcgtggtg
      841 gtcgagctgt ctaagaactt ccaggatttg tacgatcctc aggagctgct tccggagaaa
      901 ctcaaaaact gggctcagga actcggagat aacaaatacg tcaattggct tccgcagcgc
      961 gctatcggaa aactgcagga agaagccgaa cgcatggctg atgaagccta tgagcaagca
     1021 aacattgact tgtacggaac tgccgatcga gctggtgagt atgaaaagta cgagaaactt
     1081 cgtgatgagt tttcgaaagc ggccgacgag tctgaaaagt tgttggcagc cgcgcagcaa
     1141 ccagttgatg agttttcaaa gcgggttgct gaaacgcggg aacgttttga tcgcttacag
     1201 cagacctttg acaagctggt acaacggcag gcggaacttg agaaacggca ccttgatttg
     1261 ctggccgaca ttttttctga aaccaaacga gagcttgcaa agccaattcc cggtgaaact
     1321 gttgcggtcg ccaaacccga gcttgttttc gaaagcgtgc cggacgaggc gtcaagacag
     1381 atcgcacggt atgactccgg tactctggcc gccgatgaag tgacctcgcg cctgttggga
     1441 attccgatgg agctgagatt cggcagtgaa acaaaggtgg ctcaggtcat gcgatcaaag
     1501 gagaccattg cggaaaccga tgggtcaggc cggcaaacca tgaagtttct gtctcgaatt
     1561 tctctggata gcccgcgaat tgtgtacaac tacgataaag ttcgattctc tgaaagtacc
     1621 ggttgcgtgt cgctgcagtc cggtgggcgt cgcatcagcg gccgcaagcc aggtcgggct
     1681 caggtgaaac tgacggtggc cggcgctggt gccataacct atgacgccta tggaaatcag
     1741 aggttggaga cgcaggaata cttgtcgcaa caagcgattc aagtcgtatc gctcaaagat
     1801 ctggaagcgc gctttcagga tacatcgacc agaactcccg ttgagcgcat cgatctggcg
     1861 acgcccgagg gtggttcgaa cagttctccg cagttgcagt tttttgcccg catcgactgg
     1921 ggacatgaga tcacgtcccg agtactcgac ctcagtcatc tctcgattcg cgtgattggc
     1981 gacccatgtg tcacggtgat tcgcagacag aactcgatca ttcttgaaac caccaaccgc
     2041 cctggagcct gcgaactgga agtgggtacc atcgggcagg ccagattcac acgacgcatc
     2101 aaagtgacca atagcatcat tcgcggagtc gtcaggatgc cggacgggat gcagcatcgt
     2161 gcaggcactc tccagaaacc tctctcgttg cccatcgccg tcccggcaga actggctgtg
     2221 gtcattcaag gtccggcgtc gccacatgac tttctggtcg agtgggagtg cgagcgccac
     2281 ggaaatgcga tcaagtcagg aacaggcgga gtcgaagccc gag
//
LOCUS       DAORHD010000825         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56788_length_2319_cov_3.145318, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000825 DAORHD010000000
VERSION     DAORHD010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56788_length_2319_cov_3.145318"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKO06_15900"
     CDS             complement(<1..594)
                     /locus_tag="PKO06_15900"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNV71187.1"
                     /translation="MGLSARSVLLAAAVLASTAVSPVSADIKMVQQQGTRDIVLQEVK
                     VPGNLPDEVKIDEMFKRDFDISFATNKATEGDVYTFDDKIELNFTSKKDCYLTVLDFT
                     PSGQLVVLFPNQWAQDNFVKADQQIRIPPVDAKFELKSGGPVGTDRVTVIATTKPEKV
                     VDIDTKKITNAFAVIDDRKAATRQIMVIAKEDEVIKDD"
     gene            830..1261
                     /gene="arfB"
                     /locus_tag="PKO06_15905"
     CDS             830..1261
                     /gene="arfB"
                     /locus_tag="PKO06_15905"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HNV71188.1"
                     /translation="MEPLIINDVITIPATDLDWAAARASGPGGQNVNKVSSKVELRFR
                     LTSTTCLTEPVRVRLRQMAGSRLTPEGDLLIVCQVHREQHRNLDEARERLRELILQAL
                     VKPKRRVATKPTRASRERRVQAKRQHSETKRARSKRGGDDD"
     gene            1254..>2319
                     /locus_tag="PKO06_15910"
     CDS             1254..>2319
                     /locus_tag="PKO06_15910"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV71189.1"
                     /translation="MTKKPQGQALGGGCLALFGIPFLLAGLFVCWLGLSRAMVFWRSR
                     TWVPVPAMIEKIEIQESTDSEGDTTYALRGEYRFDFGGRTYRNSRIEVDEDFRSMRRR
                     LDGMFAVLDKHRQLKKPIDALVNPHNPDESLIFREFSHMMLIFPLFGLVFTIVGAGIT
                     GGGVLNIISSRRAAALVQKFPDQPWKWEGHWEGCRTSNSSLWIAVTTFFATLGGNAFI
                     WVFILATFIESDLPDIVPWIFAGLGLIGLASFLIPIGQFRHYWRYGSPVLLFERVPVS
                     PGECLRVVLLVQSKLVTTAGIVATLYCNELREESDGDGTTTVTVNRLTEIQTVHQDLN
                     RSTGRSAIPITFQIPDNQPSR"
BASE COUNT          475 a          669 c          679 g          496 t
ORIGIN      
        1 gtcgtccttg atcacctcgt cctctttcgc gatgaccatg atctgacggg tggcagcctt
       61 gcgatcgtcg atcaccgcaa acgcgttggt gatcttcttg gtgtcgatgt cgacaacctt
      121 ctcaggcttg gtcgtggcga tgacggtcac acggtcggtt ccgaccggac caccggactt
      181 cagctcaaac ttcgcatcga cgggcggaat gcgaatctgc tgatcagcct tgacgaagtt
      241 gtcctgggcc cactgattgg gaaacagcac caccagctga cctgacgggg tgaaatcgag
      301 aaccgtcaga taacagtctt ttttcgaggt gaaattgagt tcgatcttgt cgtcgaatgt
      361 gtagacatcg ccttcggtgg ccttgttggt cgcaaaagaa atgtcgaagt cgcgtttgaa
      421 catctcgtca atcttgactt cgtcaggcag attgcccgga actttgactt cctgcaggac
      481 gatatcgcga gtgccctgct gctgcaccat cttgatgtca gcagaaaccg gagagacggc
      541 agtggacgcc aggaccgcgg cggccaacaa aacggaacga gcggaaagac ccatgatact
      601 acctcccgaa aatgaattct gatcgttcaa caggatacct cgccttgaac cgcttgcgca
      661 acgggccgca ttctcagcag tgaagcgcac tccctgtcgc gacggcgaat cgttgagcga
      721 tgctaaggga aaactcaggc ccgagaatca aatgaccctg ttcgccctgc gttgcgattg
      781 aacaaccggc aacgggttga tacaatgctg ccaacaggaa cacatggcca tggaacccct
      841 gatcatcaac gatgtcatca cgattcccgc aacagacctc gactgggcgg ccgcccgcgc
      901 ctcgggcccg ggagggcaga acgtcaacaa ggtttcctcg aaggtcgaac tgcgctttcg
      961 cctgacatcg accacgtgtc tgaccgaacc ggttcgcgtg cgcctgcgcc agatggccgg
     1021 cagtcgactc actcccgagg gcgatctgct gattgtctgt caggtgcatc gtgagcagca
     1081 tcgtaacctt gacgaagcgc gtgagcgtct gcgcgaactc attctgcagg cactggtgaa
     1141 gccgaaacgg cgggtggcga ccaagccgac cagagcctcg cgcgagcgac gggtgcaggc
     1201 caaacggcag cattcggaaa caaaacgggc gcgctcgaaa cgaggcggtg acgatgacta
     1261 agaagccgca gggacaggcg ctgggtggcg ggtgtctggc tctcttcggc attccctttc
     1321 tgctggctgg tttgttcgtg tgctggctgg gactctcgcg agcgatggtc ttctggcgaa
     1381 gccgcacatg ggttccggta ccggcgatga tcgagaagat cgaaatccag gaaagtactg
     1441 attccgaggg cgataccacc tatgcattac gcggggagta tcgattcgat ttcggagggc
     1501 gcacctatcg caactcgaga atcgaggttg acgaggattt tcgctcgatg cgtcgccgcc
     1561 ttgacgggat gttcgcggtg ctcgacaaac atcgccagct gaagaagccg atcgatgcct
     1621 tggtgaaccc gcacaatcct gatgagtcac tgatctttcg cgagttttcg cacatgatgc
     1681 tcatctttcc tctgtttggg ctcgtcttca ccattgtcgg cgcagggatc accggaggcg
     1741 gagtcctgaa catcatttct tcgcgtcgtg ccgccgcact cgtgcagaag tttcccgacc
     1801 agccctggaa gtgggaaggg cactgggaag gctgtcgcac ctcgaactcg tcgctgtgga
     1861 tcgccgtgac gaccttcttc gcaacactcg gagggaatgc cttcatctgg gtgttcatcc
     1921 ttgcgacctt catcgagagc gatcttcctg acatcgttcc ctggatcttc gccggcctcg
     1981 gcctgatcgg gctcgccagt tttctgattc ccatcgggca gtttcgtcac tactggcgtt
     2041 acggctcgcc cgttctgctg tttgagcggg ttcccgtgag tccaggcgaa tgcctgcgcg
     2101 tggtgctgct ggttcagagc aaactggtga cgacggcggg aatcgtggcc acgctctact
     2161 gcaacgagct gcgcgaggag agtgacggcg acggcaccac gacggtgacc gtgaatcgtt
     2221 tgaccgaaat tcaaacggtc catcaggatc tcaaccgcag caccggccgc tccgcgattc
     2281 ccatcacgtt tcagattccc gacaatcagc cgtcgcgtg
//
LOCUS       DAORHD010000826         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56800_length_2319_cov_2.647968, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000826 DAORHD010000000
VERSION     DAORHD010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56800_length_2319_cov_2.647968"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="PKO06_15915"
     CDS             complement(<1..914)
                     /locus_tag="PKO06_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNV71190.1"
                     /translation="MLKEWYTAPELAEGLPGLTKSVTRINRRAAREGWKAQDRAGRGG
                     GREYHISSLPPETRAALVKRAQGAALPAVISTPAPIAATSGSAVEIAEPDYRHLVPNS
                     VETDSAAYDSAPDYARRKADKYLTILHACDGLFGADLIKFVETWNVEHPSMSTSYPAI
                     LRARKTKEATGASGLLARWGTREGKTRVDPKYFDFFARHYLQEGRSTLKSIWVQTLGY
                     AIHETKNRTAQEIERDFPTVETFYRLLHKAFCKSTIDRARLGEEAWNRRHGSFVSRDY
                     EALQAGEAWVSDHAQIDVMTKFDGKIVAP"
     gene            complement(939..1202)
                     /locus_tag="PKO06_15920"
     CDS             complement(939..1202)
                     /locus_tag="PKO06_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71191.1"
                     /translation="MTFAKVFEVQGEQVLVVIGNAGGKIELSFTTRIAPYRFAGIGTE
                     QRDWAHAEESLAKVDQKMAESAFKAIKAEYAEFMNSSLRQNNN"
     gene            complement(1199..1696)
                     /locus_tag="PKO06_15925"
     CDS             complement(1199..1696)
                     /locus_tag="PKO06_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71192.1"
                     /translation="MVDQTGMSIWEEIEEQMASCYGHIRLNADGYKLDLIRTFTKNRL
                     VIAVYVNGSIKGVHAQYLKPTETPCEEARRFFQPVRKRLYKPAEMKLLKKCGDLAKKM
                     IAENERIFYRPWWSDVKPLIRHLKKTCKSVTLWQESEMKVPDCETKNADAETKSAGNE
                     TEGGK"
     gene            complement(2005..2202)
                     /locus_tag="PKO06_15930"
     CDS             complement(2005..2202)
                     /locus_tag="PKO06_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV71193.1"
                     /translation="MNKRFIEIRKWLLDRGLKQIHIARKAKVSKTTVNQFCKGFTTSN
                     NLKLIFIELGCPEELLEMKVA"
BASE COUNT          430 a          692 c          604 g          593 t
ORIGIN      
        1 ggggcgacga tcttgccgtc gaatttggtc atgacgtcga tctgggcgtg atcgctgacc
       61 caagcttcac cagcctgaag agcctcataa tcacggctga cgaatgagcc gtgtctgcga
      121 ttccaggctt cttcccctaa gcgtgcccga tcgatagtgc ttttgcagaa tgccttatga
      181 agcagtcgat agaaagtctc taccgttggg aaatctctct cgatctcttg cgcggttcta
      241 ttcttggttt cgtgaatggc atacccgaga gtctgtaccc agatgctttt cagcgtcgaa
      301 cgcccttctt gcaggtagtg ccgtgcgaag aaatcgaaat acttcgggtc aacgcgcgtc
      361 ttcccttctc gggtgcccca tcgggccaga agaccactgg ccccggtcgc ttctttggtt
      421 ttgcgagccc gtaggatcgc ggggtagctt gtgctcatgg atgggtgctc cacgttccag
      481 gtctcgacaa acttgatgag atcggctccg aacaagccgt cgcatgcatg caggatcgtg
      541 aggtatttgt cagccttgcg gcgggcgtaa tcaggggcag aatcgtatgc ggctgaatcg
      601 gtctcgacgg aattcggcac caggtggcgg taatcgggct cggcaatctc aacggcagaa
      661 ccagaggtcg ccgcgatcgg cgcgggcgtc gagatgacgg cgggcaaagc ggcaccctgg
      721 gcacgtttca ccagcgcggc gcgggtctcg ggcggaagcg aggagatgtg atactcgcgg
      781 ccaccgccac gaccggcgcg atcttgagcc ttccagccct cgcgcgcggc acgtctattg
      841 attctggtta cgctctttgt caatcccggc aatccctcag caagctcggg agccgtgtac
      901 cattccttca gcatgttcag cctcttggtt cctgcgtttc agttattgtt ctgccgcaaa
      961 ctgctgttca tgaattccgc gtactctgcc ttgattgctt tgaaagccga ttcggccatc
     1021 ttctggtcaa ccttcgcgag cgattcctcg gcgtgtgccc agtcacgctg ttcggtgcct
     1081 attccggcga accgatacgg agcaatccgt gtcgtaaacg acagttcgat ctttccgccc
     1141 gcgttgccga tgaccaccag aacctgctcc ccctgcacct caaacacctt cgcaaatgtc
     1201 acttgccacc ctccgtttca ttcccggcgc ttttcgtctc agcgtcggca ttcttcgttt
     1261 cacaatctgg gactttcatt tcagattcct gccatagcgt tacactcttg caggtcttct
     1321 tcagatgcct gatcagcggc ttcacatccg accaccaggg ccggtagaaa atcctttcgt
     1381 tctcggcaat catcttcttc gccaggtctc cgcatttttt cagcagcttc atttcagcag
     1441 gcttgtaaag cctctttctc accggctgaa aaaagcggcg agcctcttcg catggggtct
     1501 cggtgggctt cagatactgt gcgtgcacac ctttgatgga cccgttaacg taaaccgcga
     1561 tcacgagacg gttcttggta aacgtcctga tcaggtcgag cttgtagccg tcggcattca
     1621 ggcggatatg cccgtagcat gaagccattt gctcttcaat ttcttcccag atactcatgc
     1681 cggtctggtc caccattcca tattcccggc ccagtaacgc gtcgtgccgc gcacagtcac
     1741 acgtcggtcg tcagtctctt caggagcccc gcatccaggg cacggaatcg attgcgcgaa
     1801 ccggcgaaag aaggtgcggc cgcattcgca gcagtaggaa gcccggctgt tcaaaacacg
     1861 tttctttcga ttctgatgcg cgtgacgatt gcgcggtgat ggcatgccgg caaattccgc
     1921 gaggcgcgat ttttcatact ctgccttact catctgaaca ttcttcattt cgatctccat
     1981 tggggttgag tcctacttgc cttttcaggc caccttcatt tcgagcagct cttcagggca
     2041 gccgagttca atgaaaatga gcttgaggtt gttgctcgtc gtgaatccct tgcagaactg
     2101 attcacggtc gtcttgctga ccttggcctt ccgggcaatg tgaatctgct tgagtcctcg
     2161 gtccagcagc cactttcgaa tttcgatgaa tcgtttgttc atccgattcc gacctcctgc
     2221 gccaggcgac gcgcatcgcg ccctagcttt cttcgagtgg tctcgatcct tgcccactcg
     2281 agcaacttca agtcttcgct gcctacaaca tgacccccg
//
LOCUS       DAORHD010000827         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56816_length_2318_cov_3.825453, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000827 DAORHD010000000
VERSION     DAORHD010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56816_length_2318_cov_3.825453"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKO06_15935"
     CDS             complement(<1..435)
                     /locus_tag="PKO06_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71194.1"
                     /translation="MVTDPIVWARDHGRSKIHDGHREGEVKATSTVPSKSEKPPVDSF
                     EKTSVPTPEVDDSSASDTSGGLKISYHFDLFYELSQKVSAKMGQKGLTQFTEVSASVA
                     ETFKGSFSLKIDALGSFLNGTDKSLSISTETAGRFMSAVEGLA"
     gene            776..2032
                     /locus_tag="PKO06_15940"
     CDS             776..2032
                     /locus_tag="PKO06_15940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV71195.1"
                     /translation="MKRVIFYLSALVILVAITIVTLIGARSGNKGQTGSRRGGGSATT
                     AVAVETSEVRVGALTDEGRFTGSLIPRSQFVVASKVAGRLKRLHVNLGDQVKQGQILA
                     EIEDDEFVQAVAQAEADLAIAKANVKEGEAQLEIARREHERVQKMRQQKVSSESEVEA
                     SLAQLKTREARHLVNLAQVQQKEAAVKTARVRLSYTRLEALWNENGPTRHVGERFQDE
                     GALLSANMSILSIYDLTSVTAVIDVVERDIAKIKIGQRATVSVAALPKQSFTGEVRRI
                     APGLNPATRQGRVEIEIPNPEFLLKPGMFAVVRLQFETHSNVAMVPLSAISRRDNREG
                     VFFVDPAQRKAHFIPIQTGIVQQDFVEIASPSLSGLVVTLGQHLLEDGMNVLLPGQPS
                     AESSGTASGAADVPDAGARKGKRKAE"
     gene            2036..>2318
                     /locus_tag="PKO06_15945"
     CDS             2036..>2318
                     /locus_tag="PKO06_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV71196.1"
                     /translation="MDLSTTSVRRPIGISMLTMIVVLFGVVSLYHLPIDLMPDITAPT
                     ISLSAQYENAGPVEIETLLTRPIEQALSAVPGVEEITSTSTEGRCSVRVT"
BASE COUNT          499 a          593 c          692 g          534 t
ORIGIN      
        1 tgccagccct tcgacggcac tcatgaacct gcctgctgtc tcggttgaaa tcgacagcga
       61 cttgtcggtg ccgttcagaa atgagcccag agcgtcaatc ttcaggctga aggagccctt
      121 gaacgtttca gcgaccgaag cgctgacttc ggtgaactgt gtcagcccct tctgacccat
      181 tttggccgaa accttctgcg agagctcgta aaagaggtcg aaatggtagg aaattttcag
      241 accgcctgag gtgtcagatg cgctgctgtc atcgacttca ggggtaggaa cggaagtctt
      301 ctcaaaggaa tccaccggcg gtttctcgga tttggatggg acggtcgagg ttgctttcac
      361 ctcgccttca cgatgaccgt cgtggatctt cgaccttccg tggtctctgg cccagacaat
      421 aggatcggta accatatgtt ttgccctcct tggcgtggtg accttttacg cggttccaat
      481 gtacctatcg acgtaattga caaaatactt gaggtgtacg gcagcgaaat ttggcggaat
      541 tatgcagaaa agagcaccac agaaggtagg cctttacatt tattaatata cattgggctg
      601 ttttttacgt ttttcatctg tttaaataga tgtttgcgtg gcaatctccc gatgaagggg
      661 cagacttgag ggaacggagc aggtcacttg tcttcagggt gacattttcg gtaccatgcg
      721 tctccgcttt cctgtaccgg catcggcaaa cttccttcgc gaaaaggacc agacgatgaa
      781 acgagtcatc ttttatctga gcgctcttgt catactggtt gcgatcacga tcgtgacctt
      841 gattggagcc cgcagtggga acaagggcca gaccggttcc cgtcggggtg gtggcagtgc
      901 cacgacggcg gtcgctgttg agaccagcga ggtgcgggtg ggggctctga ctgatgaagg
      961 tcgcttcacc gggagtctga taccccgctc acagtttgta gtggcctcga aggtggccgg
     1021 gcgattgaaa cgtctgcacg tgaatctggg cgatcaggtg aaacagggac agattcttgc
     1081 cgagatcgaa gacgatgaat ttgtccaggc tgtagcgcag gcagaagctg atctggcgat
     1141 tgccaaagcg aacgtgaaag aaggagaggc gcaactcgag attgcacgtc gcgaacacga
     1201 gcgggtgcag aaaatgcgcc agcagaaggt gtcttcggag tctgaagtgg aagcgtctct
     1261 cgcgcaactg aagacccgcg aagcgcggca tctggtaaat cttgctcagg tacagcagaa
     1321 agaggcggcc gtgaaaaccg ctcgggttcg gctttcgtat actcgtctcg aagcgctctg
     1381 gaacgaaaac ggtccgacac gccatgtcgg tgagcggttt caggatgaag gcgcgctcct
     1441 ttccgccaac atgtcgattc tttcgatcta tgatctgacc tcggtgaccg cggtgatcga
     1501 cgtggttgaa cgtgacattg ccaagatcaa gatcggacag cgagcaacgg tttcggtggc
     1561 tgcgctgccg aagcagagct tcaccgggga agtgcgacga attgccccgg gtttgaatcc
     1621 ggcgacccgt cagggaaggg tcgaaattga aattcccaac ccggagtttc tgttgaaacc
     1681 cgggatgttc gccgtggtgc gactgcagtt cgagacccac agtaatgtgg caatggtgcc
     1741 actttcagct atttcgcgcc gggacaatcg cgagggagtg ttctttgtcg atccggcgca
     1801 gcgaaaagca catttcattc cgattcagac cggtattgtt cagcaggact tcgtcgagat
     1861 tgcgtcaccg tccctttctg gattggtcgt gacgcttggt cagcatctgc ttgaagacgg
     1921 catgaacgtg ttgttgccgg ggcaacccag cgctgagtct tcgggcacgg cgtcgggtgc
     1981 ggccgacgtg cctgatgccg gcgcacgcaa gggtaagcga aaggcggagt gacccatgga
     2041 tctatcgacc acttccgtcc gtcgcccgat cggcatctcg atgctaacca tgatcgtggt
     2101 gcttttcggt gttgtctctt tgtaccatct gccgatcgat ctgatgcccg atattactgc
     2161 gccaacgatc tcactgtcgg cacagtatga aaatgccggc ccggtcgaga tcgaaaccct
     2221 gctgacccga ccaatcgaac aggcgttgag cgcagtgccc ggagtcgagg agatcacctc
     2281 gacctcgacg gaagggcgtt gctccgtgcg cgtgacgt
//
LOCUS       DAORHD010000828         2318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56824_length_2318_cov_3.395935, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000828 DAORHD010000000
VERSION     DAORHD010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56824_length_2318_cov_3.395935"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKO06_15950"
     CDS             <1..954
                     /locus_tag="PKO06_15950"
                     /inference="COORDINATES: protein motif:HMM:NF021430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HNV71197.1"
                     /translation="AGASEKREEDASPSAPEPGVVMKGWNPDTPYLKKMQAVPVDRAW
                     KAYLEQKKEHGDSAGFYLDCADFFIKAGKREIGLQVLSNVAELDLENAGLLRILAHRL
                     AQMDELELSRLTFETVLRLRPEEPQSYRDLALVVAREQNSGRALELLKEVVMRRWDRF
                     PEIEIVALMEYNSILARMKGEVPASAKLDDRLIKNLDCDMRIVMTWDTDMADMDIWVI
                     EPNGEKAFYNNKFTQIGGLVSRDFTQGYGPEEYCLRKALRGMYKIQSNYYGSSAPALS
                     SQVTVQVEIFSNFGRADEKRRAMTLRLKESRDTYTVGEVEF"
     gene            956..2080
                     /locus_tag="PKO06_15955"
     CDS             956..2080
                     /locus_tag="PKO06_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71198.1"
                     /translation="MTNDHRIRMGRADQPDSDQQQPREQGHTIVGGRPAGVTSRGGRI
                     PRAMELMLKKAAVDQEFCRQLLQKRSAASTELGLELDPAERQMLDGIPEPQLKAIIAG
                     TAVPDSQKRVLATGSAAAIVALLAQLSFSPVAARAEEQKQPSQTVTEVKQQVPGWSSL
                     GEGGARADEPFDIMPPGGARPDMPDPIDIQPVRPPRPPAPPVQVAPTVPVDPAMQPGS
                     VEFLKRAVVMSELNGSSFATGLPRLQSDLQAVITWKAPSEPDWARTIEATTAGASLDQ
                     ALRDLARELYPGQDAEIDLQAGRVTIVVGTVGGAEFASPGAEAIKPQTMPSPLNPPPF
                     DDDMNISRGIRPDFPDDMNISRGIRPDLPDLIPPDGKKRD"
     gene            2082..>2318
                     /locus_tag="PKO06_15960"
     CDS             2082..>2318
                     /locus_tag="PKO06_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71199.1"
                     /translation="MSSNPRDITILNLNLLYVRYVDSIDKELHVPLGPLYLTQALENA
                     GFRVDFRDYQLNGYAEPFALENIVAYLADPAPIIG"
BASE COUNT          479 a          678 c          723 g          438 t
ORIGIN      
        1 gcaggagcat cggaaaagag ggaggaggat gccagtccct cggctccgga gcctggcgtg
       61 gtcatgaaag gatggaaccc ggacacaccg tatctgaaaa agatgcaggc tgtaccggtt
      121 gatcgggcct ggaaggctta tctggaacag aaaaaggaac atggagattc ggcgggtttc
      181 taccttgact gcgcggattt cttcatcaag gccggcaaac gcgagatcgg cctgcaggtg
      241 ttgtcgaatg tcgcggaact tgacctcgag aatgccggac tgttgcgcat tctggcgcat
      301 cgtctggcgc agatggacga actggagctg agtcgcctga ccttcgagac ggtgctgcgc
      361 ctgcgccccg aagaaccgca gtcgtatcgt gacctggccc tggtcgtcgc gcgtgagcag
      421 aactccgggc gagcgctcga gctgctgaag gaagtggtga tgcggcgctg ggatcgcttc
      481 ccggagattg agatcgtcgc gctcatggaa tacaacagca ttctcgcccg catgaaaggt
      541 gaggttccgg catcggcgaa actggacgat cgcctgatca agaatctcga ctgcgacatg
      601 cgcatcgtca tgacctggga taccgacatg gcggacatgg acatctgggt gatcgagccg
      661 aacggggaaa aagcgttcta caacaacaaa tttacgcaga tcggcggcct ggtgtcgcgc
      721 gacttcacgc agggctatgg gcctgaagaa tactgcctgc gcaaagcgct gcgggggatg
      781 tacaagattc agtcgaacta ttacgggtcg agtgccccgg cattgtcgag tcaggtcacc
      841 gtacaggttg agattttcag caacttcggc cgggctgatg aaaagcgccg ggccatgact
      901 ctgcgactga aagagagtcg cgacacctat accgtggggg aggtcgagtt ttgatatgac
      961 aaacgatcat cgtattcgca tgggtcgcgc tgaccagccc gattctgacc agcagcagcc
     1021 acgcgagcag ggccacacga ttgtcggcgg tcgccctgcc ggagtgacca gtcgcggagg
     1081 gcgcattcca cgcgccatgg aactgatgct gaagaaggca gccgtcgatc aggagttctg
     1141 ccgacaactg ctgcagaagc gctcggcggc aagtaccgag ctgggtctcg aactcgaccc
     1201 ggcagaacga cagatgctcg acggcattcc ggaaccacaa ctgaaagcta tcatcgccgg
     1261 aaccgccgtt ccggactcgc agaagcgggt gctggccacc ggctcggcgg cggcgatcgt
     1321 cgctcttctg gcacagcttt cgttctcacc ggtcgcggca cgtgccgaag aacagaagca
     1381 accgtcgcag acggtcaccg aggtgaaaca gcaggtgccc ggctggtcat ctctgggaga
     1441 gggcggggcc cgcgccgatg agccgttcga tatcatgccc cccggcggcg ctcgcccgga
     1501 catgcctgat ccgatcgaca tccagccagt gcggccgcca cgtcctccgg cgccgcccgt
     1561 gcaggtcgcg ccgacggttc ccgttgatcc agccatgcag ccggggagcg tcgagtttct
     1621 gaaacgcgct gtcgtgatgt ctgagttgaa cgggagttcg ttcgccaccg gactcccccg
     1681 tctgcagagt gatctgcagg ctgtcattac ctggaaagcc ccatctgaac ctgattgggc
     1741 gcgtaccatc gaggcgacga cggccggtgc ctcgctcgat caggcacttc gcgatctcgc
     1801 tcgtgaactg taccccggac aggatgcgga aatcgacctg caggccggcc gggtgacgat
     1861 tgtggtggga actgtcggtg gtgcggaatt tgccagcccg ggtgcggagg cgatcaaacc
     1921 gcagacgatg ccgtcaccgc tgaatccgcc cccctttgat gatgacatga acatttcgcg
     1981 cggcattcgg ccggattttc ccgacgatat gaatatctcg cgcggtatcc gccccgacct
     2041 gcccgatctc ataccccccg acgggaagaa gcgagactga catgtcctca aacccgcgtg
     2101 atatcacgat tctgaatctg aatctgttgt acgttcgcta tgtcgactcg atcgacaaag
     2161 aactgcatgt gccgctcggt ccgctgtatc tgacacaggc tctcgagaat gccggctttc
     2221 gcgtcgactt tcgtgattat cagctgaacg gctatgccga gccgttcgcc ctcgaaaaca
     2281 tcgtggcgta tctcgctgat ccggccccga tcatcggc
//
LOCUS       DAORHD010000829         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56849_length_2317_cov_4.167993, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000829 DAORHD010000000
VERSION     DAORHD010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56849_length_2317_cov_4.167993"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKO06_15965"
     CDS             <1..969
                     /locus_tag="PKO06_15965"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNV71200.1"
                     /translation="SKKAKNARHRGKLLPLQAGLVEAMNSLRDQVRRTVPTEKRDQVQ
                     PMFGRDLVQFGKILGHWQELVGPDVSEHAWPVQLMHGRLALVCSDSQWLHHLRFLKND
                     ILQNVKQRFPGIPLQEIYGKVGSIPAHAPKQPEMPWPTWEQEPEPELPEQVSPELRER
                     ISRCARKSEARLKGFLEHGFKVCPACRSAVIPDQSTHCGVCLFRQRDTLHRRVRVLLS
                     DQPWYSFEQVHSEIPESSHEEYDIIRRELILEARDRITILGQSWVETADREVRAELER
                     EIIRELMLASQIAPNQISLSDPSIMPLLDPEWITWLNTRFDEGSPC"
     gene            963..1256
                     /locus_tag="PKO06_15970"
     CDS             963..1256
                     /locus_tag="PKO06_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71201.1"
                     /translation="MLIHLGDNEFVRLDQVVGIFPIDVVNPEIVARLGKIVTRHGMKA
                     PKSAVLTRSGKWVGSTISSEALAERGYWRPKPIRPGRRKSSESVDPDEETPQS"
     gene            1253..>2317
                     /locus_tag="PKO06_15975"
     CDS             1253..>2317
                     /locus_tag="PKO06_15975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71202.1"
                     /translation="MTTHTTEPQVDSENLPESGANGEYSARNIQVLEGLEAVRKRPGM
                     YIGGTGLDGLHHLVWELVDNSIDEVLAGHATTVHVTMHDDGSLSVLDDGRGIPVDIHE
                     KTGKSALEVILTVLHSGGKFDKNSYKYSGGLHGVGLSVVNALSEWLEVTVWRDGGTFQ
                     QSYRRGIPMGPMTVTQGAPRRGSKIRFMPDSEIFQITEFNFDVLSKRLREMAFLNSGV
                     KIVLRQTKTQKCHLFEYKGGLSSFVEFLNEAKTPLFRDVIACKKSRDNIEVEFALSYN
                     DGYSEQFCAFVNNINTVDGGTHVVGFRSALSKVFNDYLKKDPSLLGKDKSKEIKISID
                     DAREGLVGVLSIKVPEPQFEG"
BASE COUNT          549 a          601 c          633 g          534 t
ORIGIN      
        1 agcaaaaaag ccaaaaacgc tcgccatcgg ggaaagctct tacctcttca ggctgggtta
       61 gtcgaggcca tgaattcgct gcgtgatcaa gtgcggcgaa cggtgcccac agaaaagcgt
      121 gatcaggttc agccgatgtt cggccgtgat ctggtgcaat tcggcaaaat ccttggccac
      181 tggcaggaac ttgtcgggcc cgatgtgtct gagcatgcgt ggccggttca actgatgcat
      241 ggccgactgg cactggtttg cagtgactct cagtggctgc atcacttgcg ttttctgaag
      301 aacgacattt tgcagaacgt caaacagaga ttccccggaa tcccgcttca ggaaatctac
      361 gggaaggttg gttcaattcc cgcgcacgca ccgaaacagc cagagatgcc ctggccaacg
      421 tgggagcagg aaccggagcc agaactcccg gagcaggttt ccccggaact gcgagaacgt
      481 atctcgcgct gcgcacgtaa gtctgaagca cgactcaaag ggtttctgga acatggattc
      541 aaagtctgtc ctgcctgccg aagtgctgtg attcctgacc agtcgactca ctgtggagtc
      601 tgtctgtttc ggcaacgaga caccttgcat cgacgggtac gcgtactgct ttccgatcaa
      661 ccctggtaca gctttgaaca ggttcactct gaaattccag aatcgtcaca tgaggagtat
      721 gacatcatcc gtcgcgaatt gattctggaa gcgcgtgatc gcatcacgat cctgggacaa
      781 tcctgggttg aaacggctga ccgtgaggtg cgcgcggaac tcgagcgcga aatcattcgc
      841 gagctgatgc tggcgagcca gatcgctccg aaccagatct ccctttccga tccatcgatc
      901 atgccattgc tcgatccgga atggattacc tggctgaaca ccagattcga tgaaggtagt
      961 ccatgttgat tcatctgggc gacaatgaat ttgtccgtct cgaccaggtg gtcggaatct
     1021 ttccgatcga tgttgtcaac cctgagatcg ttgccagact gggaaagatt gtcacccggc
     1081 atggcatgaa ggctcccaag tctgctgttt taacccgttc cggcaagtgg gtgggctcga
     1141 ccatctcatc agaagcgctt gccgagcgcg gctactggcg tccgaaacct atccgtcctg
     1201 gtcgaaggaa atcgtccgag tctgttgatc ccgatgagga gacaccgcaa tcatgactac
     1261 acatacgaca gaaccccagg ttgattctga aaatttgcct gaatccgggg cgaacggaga
     1321 atactctgcg cgcaatattc aggttctcga aggactcgaa gccgttcgca aacgcccggg
     1381 catgtatatc ggtggtaccg ggcttgacgg cctgcatcac ctggtctggg aactcgtcga
     1441 caacagtatc gatgaagttc ttgccggtca tgcaaccacg gttcatgtca cgatgcatga
     1501 cgacggctca ctgtcggtgc tcgacgatgg ccgcggtatt cctgtcgaca ttcacgaaaa
     1561 aaccgggaaa tcagctctcg aagtcattct gacggttctg cactcgggcg gcaagtttga
     1621 caagaacagc tacaaataca gcggtggtct gcatggcgtc ggtctttccg tcgtgaacgc
     1681 cctgtcggaa tggctcgaag tgacggtctg gcgtgatggc ggcacctttc agcagtcgta
     1741 tcggcgcgga attcccatgg ggccgatgac ggtcacgcag ggtgcgcctc gtcgcggcag
     1801 caaaatccgc ttcatgccag attccgaaat ctttcagatc acggaattca attttgatgt
     1861 gctttctaaa cgcctgcgtg aaatggcttt tctgaactcg ggcgtcaaga tcgttctgcg
     1921 ccagacgaaa acccagaaat gtcatttgtt tgagtataaa ggcggtctct cgagcttcgt
     1981 cgagttcctg aatgaagcga aaacaccgtt atttcgcgat gtcattgcct gcaagaagtc
     2041 gcgtgacaac atcgaagtcg aatttgctct ctcgtacaac gatggttaca gcgaacagtt
     2101 ctgtgcgttt gtgaacaata tcaataccgt tgatggtggt acgcacgtcg tcggcttcag
     2161 atcagcactc agtaaagtat ttaacgatta cctgaagaaa gatccttcat tgcttggcaa
     2221 ggacaagagc aaagaaatca agatttcgat cgatgacgcc cgcgaaggat tggtgggcgt
     2281 gctttcaatc aaggtcccgg aaccacagtt tgaagga
//
LOCUS       DAORHD010000830         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_56864_length_2317_cov_3.445181, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000830 DAORHD010000000
VERSION     DAORHD010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56864_length_2317_cov_3.445181"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="PKO06_15980"
     CDS             <1..2212
                     /locus_tag="PKO06_15980"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV71203.1"
                     /translation="NRDAYDRYLSTYRVYQDALSRGLPEEQIKKALGEYSAAKSAYEK
                     TIVAPDATQAQTPAAAIAMGADGSPALELPGSVITTVVPSTIENSCGLQASGVPSLSS
                     ELRGILEDLWDPRGRAKPDLTIQRLENYIKAHPSDVAVNYARYELAKAYEYLKNDPGK
                     AWNVLKAMTTDPSAGRYANLATERLRYLEGMAQHNKWKSALNTKYQEMNDKYASYRNT
                     SWLAFPVKGVRFASYLGKLIGFNKAQSDYKKFQLWYEELAGKFAPAPDVVFDQFKVPA
                     GSGDPEAHVRLIYTNSEAWYTRWKLINEAKHSIDVQYFIVDGDIFGYSLAGLFLRKAR
                     EGVKIRFMMDARGTKGFTRKLMGQDYMQELSEFPNVEIKVFNPVHQNLLTMWGNLRRI
                     MASNHDKIVVVDGEYAIIGGRNISKDYFVDPADHPHAYRDCDVVIRSTEVAKQLDLAF
                     DEEFAMLKSLSITKDLFGNVDIVTKPLEAAFQAMDYYVRGNGLLKPMPGNDKVFMKNM
                     EKFNEELAVYKHLIGFTGFDPLDGAHEAPVKIIDKHSLGGPRNDITDQLVKFIDGSRK
                     EILIQNPYVVLTERAEAALKRAAKRGVRILMHTNSPISTDSLATQAMFYADWKRILKD
                     IPTLSIYTYYGDRKLHAKNFVFDGQIGVVGTYNMDYLSEEVNSEVVAAINSKSFATEL
                     RNGILEDMRNSKRYAIEVGADGEVKEVFGPDQLQSSKSGMIRFLSNFGFLKPLI"
BASE COUNT          558 a          613 c          637 g          509 t
ORIGIN      
        1 caatcgggat gcctacgatc ggtatctgtc gacataccgc gtgtatcagg atgcgttgtc
       61 ccgcggactt cccgaagagc agatcaagaa agcgctgggc gagtattctg ctgcgaaatc
      121 cgcctatgaa aagactatcg tcgcccccga tgccacgcag gcacagaccc cggcagcggc
      181 aatagcgatg ggtgcagatg gctctccagc tcttgagctt ccaggctcgg tcattaccac
      241 cgttgtcccc tcgacaatcg aaaactcttg tggcctgcag gcatcaggag ttccttccct
      301 gtcttcagaa ctccgtggca ttctcgagga tctctgggac ccgcgcggtc gtgccaagcc
      361 tgatctgacc attcaacgcc tggaaaacta tatcaaggct catccgtcag atgtggccgt
      421 gaactatgcg cgttacgagt tggccaaagc atacgagtat ctgaaaaacg atcccggaaa
      481 ggcttggaat gtgctgaagg ccatgaccac cgacccgtcg gctggtcgtt atgcaaatct
      541 ggccaccgag cgcctgcggt atcttgaagg catggcgcag cacaacaaat ggaaatcggc
      601 cctgaatacc aaataccagg aaatgaacga caagtatgcc agctatcgca atacatcttg
      661 gctggcgttt ccggtaaagg gggttcggtt tgcctcgtat ctgggcaaac tgatcggttt
      721 caacaaagcc cagagcgatt acaagaagtt tcagctctgg tatgaagaac tggcgggcaa
      781 gttcgcgcct gcccccgatg tggtgtttga tcaattcaag gttccggcgg gcagtggtga
      841 cccggaagca cacgttcgtc tgatctacac caactctgag gcgtggtata cccgctggaa
      901 gctgatcaac gaagccaagc attccattga tgttcagtat ttcatcgtag atggcgatat
      961 cttcggctat tcgctcgcgg ggctgtttct gcgcaaagcc cgtgaaggtg tcaaaattcg
     1021 cttcatgatg gatgctcgag gaaccaaggg cttcacccgc aagctcatgg gccaggacta
     1081 catgcaggaa ctgtcagagt ttccgaatgt cgagatcaag gtcttcaatc cggttcatca
     1141 gaacctgctg acgatgtggg gaaacctgcg tcgcatcatg gccagcaatc atgacaagat
     1201 cgtcgtggtc gatggagaat acgccatcat cggtggccga aacatctcga aagattactt
     1261 tgtcgatcct gccgaccatc cgcacgccta tcgcgattgc gacgtggtca tccgcagcac
     1321 ggaagttgcc aagcagcttg atctggcctt cgatgaagaa tttgccatgc tcaagtcgct
     1381 cagtattaca aaagacctgt ttggcaatgt cgatatcgtg accaagcctc tggaggctgc
     1441 tttccaggca atggattact acgtgcgcgg gaacggtctg ctgaagccga tgcccggaaa
     1501 cgacaaggtg ttcatgaaga acatggaaaa gttcaacgaa gagcttgccg tttacaagca
     1561 cctgatcggg ttcaccggtt tcgacccact tgatggcgcg catgaagcac cggtgaagat
     1621 catcgacaaa cattcgctgg gcggcccacg caacgacatc accgatcagc tggtgaagtt
     1681 catcgacggt tcgcgcaagg aaatcctgat ccagaatccg tatgtcgttt tgaccgagag
     1741 agccgaagcg gctttgaaac gcgccgcgaa gcgcggagtg cgcattctca tgcacaccaa
     1801 cagccccatc tcgacggaca gtctggcgac acaggccatg ttctatgcag attggaagcg
     1861 cattctcaaa gacattccga cgctgtcgat ctacacctat tatggcgacc gcaagctgca
     1921 cgcgaagaac ttcgtcttcg acggccagat cggtgtggta gggacttaca acatggacta
     1981 tctgagcgag gaagtgaact ccgaggtggt tgctgccatc aactcgaagt catttgcgac
     2041 cgagctgcgc aatgggattc tcgaagatat gcgcaactcg aaacggtatg cgatcgaggt
     2101 cggagccgac ggtgaagtca aggaagtgtt cggtccggac cagctgcaga gcagcaagag
     2161 cggcatgatt cgctttctgt ccaacttcgg tttcctgaaa cctttgatct gatcatcgaa
     2221 cgagtgagaa aagacccgct tcggcgggtc tttttcattg aggcttactc ggacaagtct
     2281 gcgtggcgaa ctggcctgag atggttgcgt cgtcttg
//
LOCUS       DAORHD010000831         2313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57014_length_2313_cov_3.374668, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000831 DAORHD010000000
VERSION     DAORHD010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57014_length_2313_cov_3.374668"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="PKO06_15985"
     CDS             <1..1253
                     /locus_tag="PKO06_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71204.1"
                     /translation="QISSVWGIAFLLVYSIDRFAAFLYQQNENNPSLFHLRHPLGNID
                     TAVYHFTYTGGVRDFWPGTAMLERDLINVVKRYLLTQRSGYLWTERQGETLLQVWEKV
                     SDQLVLVAGQVRIPARPPLLRDAVLLLLLLAVVSISLNLLMADLLSSLFVAPLRVLSE
                     AVEDVKHGDFGVRIDIRSTDELEEVGDAFNRMIEALQQRERLKRFVPAPVVQRLEDAT
                     VPTTSSTARADDEVEVVVLASDIRGFTRLSETVPPDILVRALNQYFTLMQDAVTQQNG
                     RVLKLIGDAILAVFLPDASGTAPVAAALQAARDMRAQLRRFNQQRAAEGLFSLENGIG
                     LALGQALPIELGQSGSRREFTLFGAPLTEAQALESLSAHGRHSRIVAGTAFVDRLGVR
                     GQCTTITGDGESQPDGWELPEVDA"
     gene            1250..>2313
                     /locus_tag="PKO06_15990"
     CDS             1250..>2313
                     /locus_tag="PKO06_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71205.1"
                     /translation="MNVRVASLRPPFLLVISAWIFFVALPCVIIWNVWTRGIAWEEER
                     DSERRRHRLTQELHAFARELELPTFLDSQCPPRLNAIWQRDFTGNRQKLDLSAASLAR
                     RHEYLDRTLPFPVAAMIWHGPDTHECTISAGKLWDTPLSKRGLLQLMALANGQARRRR
                     QPGSAPAWLTQLERLTEEQEANLVRGLGSTLFGDVVRARLQPGRSVALLSRFFGGGRL
                     HAFYLPFYQDTTETSPVLGGCLLLIRERDISPERLWRTVARQPLHQGHSRTVVLRRDL
                     PSVASDPVLLPRFHADPQGEHFEGRPAEWFLALTNARGTLRSLNDASSLLLRVSYPSS
                     RLRSPMRAWLPSLEVGIQLA"
BASE COUNT          414 a          741 c          665 g          493 t
ORIGIN      
        1 aacagatttc atccgtgtgg ggcatcgcat ttctgctggt gtactccatc gatcgcttcg
       61 ccgcgtttct ctatcagcag aacgagaata acccgagcct ctttcacctg cgtcaccccc
      121 tgggcaacat cgacaccgcg gtctaccact tcacctacac cggaggggtg cgagacttct
      181 ggccgggcac ggcaatgctt gagcgtgatc tgatcaacgt cgtcaagcgc tatcttctga
      241 cacaacgaag cggatatctg tggaccgagc gccagggaga gaccctgctt caggtttggg
      301 aaaaagtcag cgatcagctg gtgctggttg ccggtcaggt ccggattccc gcgcgaccac
      361 cgctgttgcg cgacgcggtg ctgctcttgc ttctgcttgc cgtggtcagt atctctctga
      421 atctgctgat ggcagatctg ctgtcatcct tgttcgtcgc cccgctgcgc gtgctgtccg
      481 aagccgtcga agacgtgaag cacggcgact tcggggttcg tatcgacatt cgctccacgg
      541 acgaactcga agaagtcggc gacgctttca accgtatgat agaggctcta caacaacgcg
      601 aacgtcttaa acgattcgta ccagcgccgg tggtacagcg tctggaagac gccaccgtac
      661 ccacgacatc atcgacggcg cgagccgacg acgaggttga agtggtggtt ctggcctcgg
      721 acatacgggg cttcacccgc ctgagcgaaa cggtgccccc ggacattctg gtgcgcgcgc
      781 tcaatcagta tttcaccctc atgcaggacg ccgtgacaca gcagaatggt cgcgttctca
      841 aactgattgg agacgcgatt cttgccgtct tcctgcctga cgcatcggga accgcgccgg
      901 tcgcggccgc gctccaagcc gctcgtgaca tgcgcgcgca gttgcgtcgg ttcaaccagc
      961 agcgtgcggc agagggcctt ttttcgcttg agaacggaat cggtctcgct ctcggtcaag
     1021 cgctgcccat cgaactgggt cagagcggca gtcgtcgaga attcacgctc ttcggcgctc
     1081 cactcacgga agcccaggca ctcgaaagcc tttcggcgca tggacggcat tcacgcatcg
     1141 tcgccggtac cgcgtttgtc gatcgtctgg gcgtgcgcgg ccagtgcacg acaattacgg
     1201 gtgacggcga aagtcagcct gacggttggg aactgccgga ggttgatgcg tgaacgtacg
     1261 agtcgcttct cttcgccctc ctttcctttt ggtcatttca gcctggatct tttttgtggc
     1321 gctcccctgc gtcatcatct ggaatgtctg gactcgcggc atcgcgtggg aggaagaacg
     1381 cgacagtgag cggcgacgac accgcctgac gcaggaactg cacgcctttg cccgtgaact
     1441 cgagcttccg accttcctcg acagccagtg cccgccacgt ctcaatgcga tctggcaacg
     1501 agatttcacc ggtaacagac agaagctcga cctttcggcg gcttcccttg cgcgacggca
     1561 cgaatacctg gatcgtactc tgcccttccc ggtcgcggcc atgatctggc acggccctga
     1621 cacgcatgag tgcacgataa gcgccggcaa actctgggac accccgctct cgaaacgcgg
     1681 actgctgcaa ctgatggctc tggccaacgg tcaagcgaga cggcggagac aacccggttc
     1741 tgcgccggcc tggctgaccc agctcgagcg gctgacggaa gaacaggaag ccaatcttgt
     1801 tcgtgggctc ggcagcacgc tcttcggcga tgtcgtccgc gctcgcctgc agcccggtcg
     1861 cagtgttgct ctgctttctc gctttttcgg tggcggtcga ctgcacgcct tctatctccc
     1921 gttttatcaa gatacgaccg agacgtctcc ggttctgggg ggatgcctgc tcctgatccg
     1981 tgaacgagac atctctcccg aacgtctctg gcgcacggtc gcacgtcagc cattgcatca
     2041 gggccactct cggaccgtcg tcttgaggcg tgacctcccc tcggttgcct cagatccggt
     2101 ccttctcccc cgcttccatg ccgatccgca gggagaacac ttcgagggca gaccagcaga
     2161 gtggtttctg gccctgacaa acgcgcgcgg tactctacgg tcgctgaatg acgcctcatc
     2221 actgcttctg cgtgtcagtt acccgtcatc aaggctgcgc agccccatgc gtgcctggct
     2281 gccctccctc gaagtcggca tccagctcgc ctt
//
LOCUS       DAORHD010000832         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57121_length_2310_cov_2.931264, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000832 DAORHD010000000
VERSION     DAORHD010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57121_length_2310_cov_2.931264"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKO06_15995"
     CDS             <1..573
                     /locus_tag="PKO06_15995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71206.1"
                     /translation="IRLAGELEIRDDRARAAFAEDLRQLTEFLDRTLLAYRVGSHQAT
                     FEFVALDLATCLEPLIQDVFGPLGEREKLVTTRVQAVTWSVDRTFFSMLLTNLLTNAR
                     KYGTEIEVVLEPLDTGFLLRVKNRVTTALQPSDLPLLSQPFFRPDQSRQPGTSGSGLG
                     LFIARQIAEAHRFSFDLSLEAEIFSVTIRA"
     gene            855..1415
                     /locus_tag="PKO06_16000"
     CDS             855..1415
                     /locus_tag="PKO06_16000"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HNV71207.1"
                     /translation="MPNLSAYGSNPDQQAVERLLGRPARVPFTVLKRCPDGSPQVVQA
                     EPVFQEDGIWKPFPTIYWLLCPKLRLAISHLEQGGFLKSMLSRLEQDSAFREAFLRGQ
                     SAVCECRVRRAAQILPQPLSENMAKVLRETSIAGSHHLLGLKCLHAHAAHTLAGGENP
                     VGEAILAATGSCDGIHQESTGGGATE"
     gene            1412..2251
                     /locus_tag="PKO06_16005"
     CDS             1412..2251
                     /locus_tag="PKO06_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNV71208.1"
                     /translation="MIRGIYTSASGMLCEIVRQDMVANNLANVDTPGFKRDQGIFKEL
                     PTMMLRKVNDGKLYPPRPLYKFPKIGKLGTGAIIDESHTDFSAGKYQFTDNDLDCALE
                     NNKAFFVMETARGTRFTRDGSFTLDQEGFLVSHHGDYVLAEPEPPVESAGPLFDANGN
                     PTGEYTRVQVALTDKMTIDTDGRVIVNGRPSFRLLRGMASDRKAFRKDQSNDFVRAYG
                     DVNRAGGKVKPGYLEKPNFSLVEEMVKMIEVSRAYEANSKVIQAHETLLDRVVNSVGP
                     SRR"
BASE COUNT          480 a          664 c          659 g          507 t
ORIGIN      
        1 atccgactgg ccggagaact cgagattcgc gacgaccggg ctcgtgccgc ctttgccgaa
       61 gacttgcgcc agttgaccga gtttctcgat cgcaccctgt tggcgtatcg cgtcgggtcg
      121 caccaggcca cctttgagtt cgtcgctctc gacctggcga cttgtctcga accgttgatt
      181 caagacgtgt ttggccccct gggggaacgc gagaagctgg tgacgacccg cgtgcaggcc
      241 gtcacctgga gtgtcgatcg cacctttttc tcgatgctgt tgacgaatct tctgacgaac
      301 gccagaaaat acggtaccga gatcgaagtc gtgcttgaac cgctcgatac ggggtttctg
      361 ctgcgcgtga aaaaccgggt gacgactgcc ctgcaacctt cggatcttcc tctgctttcg
      421 cagcccttct tccgccctga tcagagccgt cagccaggaa cgagcggatc gggacttggc
      481 ttgttcattg cccgccagat tgccgaagca catcgctttt ctttcgatct gtcgctcgaa
      541 gccgagattt tctcagtcac catccgagcc tgactcttcg acactcttcc gacaagctcc
      601 tcggcgagcc tgaccgtctc ttggcacacg gctccttcga ttccattctg ctcaaggaca
      661 attcatactc tcctgttctc tccccgccgt gtcggcgaat cgttgagtga cgccgagggt
      721 gaaacccaat ctggagaatg aaatgcccca gatctttcgt tgccgtcgtt catgaacgtg
      781 cgacggaaaa gtgtctgtac cccttcggta ttcggtgcga aatgtcgatg ttccttatag
      841 gaaaggggaa aaacatgccc aacctgtcgg cttatggttc aaatcctgat caacaggcgg
      901 ttgaacgatt gctgggccgg ccggcacgtg ttcccttcac tgtgttgaag cgttgccctg
      961 atggttctcc gcaggtcgtc caggcagagc cggtttttca ggaagacgga atatggaagc
     1021 cctttccgac gatttactgg ctgttgtgcc cgaaactgcg tctcgccatt tcgcatcttg
     1081 aacagggagg ctttctcaag tccatgcttt ccagactgga acaggattca gcgtttcgcg
     1141 aggcctttct gcgcggacag tcagctgtct gtgagtgtcg cgtgcgccga gccgcccaga
     1201 ttctgcccca gcctctgtcg gaaaacatgg cgaaagtatt gcgggaaacg agtattgcgg
     1261 gcagtcacca tctcctgggc ttgaaatgcc tgcacgcaca tgcggcgcac acgcttgccg
     1321 gtggtgaaaa cccggttggt gaagcaattc tcgcagcaac cggctcatgc gacggcatcc
     1381 atcaggaatc cacgggcgga ggggcaaccg aatgattcgc ggcatctaca cgtcagcgtc
     1441 cggcatgctc tgcgagattg tgcgccagga tatggtcgcc aacaatctgg cgaacgtcga
     1501 tacgcccggg ttcaagcgcg atcagggcat tttcaaggaa ctgcccacca tgatgctgcg
     1561 caaggtcaac gacggcaagt tgtatccgcc gcggccgctc tacaagtttc ctaagatcgg
     1621 caagctcgga acgggggcga ttatcgacga gtcgcacacc gacttttccg ccggaaaata
     1681 tcagtttacc gataacgacc tggactgcgc gctcgagaac aacaaggcgt tctttgtcat
     1741 ggaaacggcg cgcggaaccc ggttcacccg cgatggctcg tttacgctcg atcaggaagg
     1801 ctttctcgtg agccatcacg gagattacgt tctcgcagaa cctgaacctc cggtcgaatc
     1861 ggctggcccg ctgttcgatg cgaacgggaa tccgacgggc gaatacaccc gggtgcaggt
     1921 cgcactgacc gacaagatga cgatcgatac tgatggacgc gtcattgtga atggccgacc
     1981 gtcattccgt ctgctgcgag gcatggcctc tgaccggaag gcgtttcgca aggatcagag
     2041 caacgacttc gtgcgcgcct acggcgacgt gaaccgcgcc gggggcaaag tgaagccagg
     2101 gtatctcgaa aaaccgaatt tcagtctggt tgaagaaatg gtcaagatga ttgaagtctc
     2161 tcgcgcctat gaagccaatt cgaaggtgat tcaggcgcac gaaaccctgc tcgaccgcgt
     2221 ggtgaactcg gtcgggccat cgcgacggta agcgtcgggc gaaagggagg taaccaccta
     2281 tgatgagaag tctgtattcc gcggccaccg
//
LOCUS       DAORHD010000833         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57135_length_2310_cov_2.580044, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000833 DAORHD010000000
VERSION     DAORHD010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57135_length_2310_cov_2.580044"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..662
                     /locus_tag="PKO06_16010"
     CDS             48..662
                     /locus_tag="PKO06_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71209.1"
                     /translation="MRSELQNFVSCCRGWALPVLLATLFPLFGCTGCRKVPEPAPEPT
                     RSPGRLVSIESLAEATGTQTLFERLQLQKVHQDYTKSYLDLMQKINQTNTDGLNVKEQ
                     LNTFSNYSYAFRRYRDVMNKIANADKEMKKKCFSIMRSLIVSVVVYDKKTKKKMFKFD
                     AQKLLQESIITELPKCPAGGEYSIYYKDGQRLLQCSRHGTLRSH"
     gene            complement(705..899)
                     /locus_tag="PKO06_16015"
     CDS             complement(705..899)
                     /locus_tag="PKO06_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71210.1"
                     /translation="MHAFTTPGIGDDLSMKDLIITVGLTVVFSVGVGVLFVHTLVELF
                     DILIKTGGKDDSHRRMKPSA"
     gene            complement(1000..>2310)
                     /locus_tag="PKO06_16020"
     CDS             complement(1000..>2310)
                     /locus_tag="PKO06_16020"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV71211.1"
                     /translation="PGQPDDDDDEGRPQTPFDDFFDQFFKNNPFGRKFQPPRGPNKLE
                     GAGTGVVIDPNGTILTNNHVIKGATKITAKFSDGKELEAKVVGQDPQTDLAVIKVESK
                     TPLPAASFADSDKVEVGQWCIAIGNPLGLEQTVTVGVVSAMGRSGIGASMIEEFIQTD
                     ASINPGNSGGPLVDLDGRVIGINTLIFAAPGSGIGFAIPSNLAKRVATQITASGSVER
                     PYIGITMAPVTADLAEHYNLGDRNGAVVMELKDDTPGGKAGLRQMDIIRSIDGKPMTS
                     TSDVQRYVLNRNVGDTIEATVLRNGQEMKIPIKLERMPRTFGLRDPEDLAESPLKGST
                     EDSIQARKLGMRYRTITPELAKKLNFDKEKGLFIEDVKSGSAADQAGLQPRDIITQVN
                     GVAVHDEKTLQLALRAATGGKKSSVFVVVRDGSPLFVVVPHEAK"
BASE COUNT          489 a          632 c          653 g          536 t
ORIGIN      
        1 gatgcgttga cagggcgtgg agccgacggt aagatgtcat attccgcatg cgatcagaac
       61 tccagaactt cgtttcctgc tgtcggggat gggcgctgcc agtattgctg gcaaccctgt
      121 tcccgctctt cggttgcaca gggtgccgca aggttcccga accggcacca gagccgaccc
      181 gaagtcctgg acgactggtt tcgattgaat cgctggcgga agccaccgga acacaaacgc
      241 tgttcgaacg tcttcaattg cagaaggtgc atcaggatta cacgaagtcc taccttgatc
      301 tgatgcagaa gatcaatcag accaataccg atgggctgaa tgtgaaagag cagctgaata
      361 ccttcagtaa ctattcctac gcatttcggc gctatcgcga cgtgatgaac aagatcgcca
      421 atgccgacaa ggaaatgaag aaaaagtgct tctcgatcat gcgctcactg atcgtcagtg
      481 tcgtcgtcta cgacaagaaa accaagaaga agatgttcaa gtttgacgcg cagaaacttc
      541 ttcaggaatc cattatcacc gagctcccga aatgccccgc cgggggggaa tactcgattt
      601 actacaagga tggccagcgg ctgctccagt gttcccgcca cgggaccctg cgctcacatt
      661 gagaggcgaa acaaagagtt tgcgaatcac ggttctgact cccttcaggc gcttggtttc
      721 atgcgacggt gagaatcatc tttgccgccg gttttaatca gaatgtcgaa cagttcgacc
      781 agggtgtgaa cgaagagaac tccgacgccg accgaaaaaa ccacggtaag gccgacagtg
      841 atgatcaggt ctttcattga caggtcgtct ccaatacccg gtgtggtgaa ggcatgcaca
      901 tggttcctcc cttatcgacc ggcaaccgat gatcgttaac gaatggaaac agaaaagaaa
      961 aaccccgctc cttggagcgg ggtttcgagc tcaggggacc tattttgcct cgtgtggcac
     1021 cacgacgaag agcggactgc cgtctcgaac gacgacgaac acagaactct tcttcccgcc
     1081 agttgcagct ctcagagcca gttgcagtgt cttctcatcg tgcacggcaa cgccattgac
     1141 ctgagtgatg atgtcgcggg gttgcagacc ggcctggtca gcggcggagc ctgacttgac
     1201 atcctcgatg aacaaccctt tttccttgtc gaagttgagc tttttggcca gttccggagt
     1261 gattgtccga tagcgcatgc caagcttccg agcttgaatg ctgtcttctg tactgccttt
     1321 cagggggctt tccgccaggt cctccgggtc gcgcaggcca aaggtgcggg gcatccgctc
     1381 gagcttgatc ggaatcttca tctcctgacc gttgcgtaga acggttgctt cgatcgtgtc
     1441 gcccacgttg cgattcagaa catagcgctg cacgtcgctg gtggaggtca tcggcttccc
     1501 gtcgatcgag cgaatgatat ccatctgacg cagaccggct ttcccgccag gcgtgtcgtc
     1561 tttcagttcc atgaccactg cgccgtttcg gtcgccaaga ttgtagtgtt ctgccaggtc
     1621 ggctgtgacc ggtgccatcg tgatgccgat gtacgggcgt tcgacggaac ccgaggccgt
     1681 gatttgtgtt gccacccgtt tcgccagatt cgacggaatg gcaaagccta ttcctgagcc
     1741 gggagctgcg aagattaggg tgttgatgcc gatgacgcga ccatccaggt cgacaagcgg
     1801 accgccggag ttgccgggat tgatgctggc atcggtctga atgaactctt cgatcatcga
     1861 ggccccgatt cccgagcgcc ccatggcact caccacgccg acggttaccg tctgctcgag
     1921 tccaagcggg ttgccgatgg cgatacacca ttgtccgact tccactttgt cagaatcggc
     1981 aaatgatgcg gccggcaggg gagtcttaga ttcgactttg atgacagcca gatcggtctg
     2041 tgggtcttgt ccgaccactt ttgcctcgag ttccttgccg tcggaaaact tggcggttat
     2101 tttcgtcgca cccttgatga cgtgattgtt ggtcagaatg gtgccgttcg ggtcgatcac
     2161 gacaccggtt ccggctccct cgagcttgtt gggcccacgc gggggttgaa acttgcgtcc
     2221 gaacggattg ttcttgaaga actggtcgaa gaagtcatcg aacggtgttt gcggtctgcc
     2281 ttcgtcgtcg tcatcatcgg gttgtcccgg
//
LOCUS       DAORHD010000834         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57367_length_2304_cov_2.881281, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000834 DAORHD010000000
VERSION     DAORHD010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57367_length_2304_cov_2.881281"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PKO06_16025"
     CDS             <1..561
                     /locus_tag="PKO06_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNV71212.1"
                     /translation="DFMSRRVPVDKFAMIYAGAQKNAGPAGATIVILREDMLAKCPES
                     IVAYLDYRLHAKEDSLYNTPPVFSIWGIKLVMEWVKQQGGLPAMEKLADERAKIIYGA
                     IERSKGFYNCPVPKACQSKMNIVFRMKTEELEGKFLEEAKKRKLDGLKGHRSVGGCRA
                     SVYNAMPVDGAKALAQLMDDFAKANG"
     gene            749..1189
                     /locus_tag="PKO06_16030"
     CDS             749..1189
                     /locus_tag="PKO06_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71213.1"
                     /translation="MGYRKERSFVKRRLFRYGLSLIAMMTLALVLAILAHAQDLRTRP
                     SLVTSPSSRLTENEWLIANRTLIRANKTRPDAQALFAEFRHYTGWTREESYARARTFV
                     ATTDLTVDDMVDIGVMLLFRADYNPYPVFTEVARTLTPYQPFNR"
     gene            1376..>2304
                     /locus_tag="PKO06_16035"
     CDS             1376..>2304
                     /locus_tag="PKO06_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV71214.1"
                     /translation="MMELIRVCIVTDDRNTFEACSEMLSSFSPGEFKIEWLAGFESAI
                     EELKAFSGGVFLVDEFLKTLSCWDLLATIRQYPGIPPIIVLSHPAAGADTISPTEHLS
                     LEERAMREGAFDLLPMPQLTPVLLVKMVRYAHRHSHTIADLATSEERYALAMKGTTDG
                     IWDWNLETDEVFFSSRWKSLLGLAEDSIGSRPDEWFQRVHPADVLRLKGQLASHLAGH
                     TPHFESEYRVLHHDGTYRWVISRGASVRGPDGKAYRMVGSQTDISGRKKVEQEILHRT
                     VFEMLIRSISTKFINCPSKQIREAIAESLKSIG"
BASE COUNT          489 a          720 c          615 g          480 t
ORIGIN      
        1 gatttcatga gtcgccgcgt gccagtcgac aagttcgcca tgatctatgc cggcgcccag
       61 aaaaacgccg gcccggcggg cgccaccatt gtcattctgc gcgaggacat gctggccaaa
      121 tgccccgaga gcatcgtcgc gtatctcgac tatcgcctgc atgccaaaga agattcgctc
      181 tacaacacgc ctcccgtgtt ttccatctgg ggcatcaagc tggtcatgga atgggtaaag
      241 cagcagggcg gcctgcccgc catggagaaa ctcgctgatg agcgcgcaaa gatcatctac
      301 ggtgccatcg aacgcagcaa gggcttctac aactgccccg ttcccaaggc atgccagtcc
      361 aagatgaaca tcgttttccg gatgaaaacc gaagaactcg aaggcaagtt cctcgaagaa
      421 gccaagaaac gcaagctcga cggtctcaag ggtcaccgca gcgtcggtgg ctgtcgcgct
      481 tcggtctaca acgcaatgcc ggtcgacggg gccaaagctc tggcccagtt gatggacgat
      541 ttcgcgaaag caaacggctg aatttgagcc cttcggggct ccccgaccat ccggcgccaa
      601 ttgccgggtg ttcagatata ccggccaggt cactcgaccc ggccggtttt tctttctccc
      661 ttcttcccca tcgaaaattc ccttccgttt ccctgttatt tccggtacat taatgaatga
      721 gaggaagttg ccgaagggaa aatcgcccat ggggtaccga aaagagcgct cattcgtcaa
      781 acgtcgtctg tttcgctacg ggctgagcct gatagccatg atgacgctcg cgctggttct
      841 ggcaattctt gcccacgctc aagacctgcg aacccgtcct tccctggtca cttcaccgag
      901 ctcccgactc accgaaaacg agtggctcat cgccaaccgc accctgattc gcgccaacaa
      961 gacgcgcccg gacgctcagg ccctgttcgc cgagtttcgc cactacacgg gctggacccg
     1021 cgaagaatcg tatgcccggg cccgcacttt tgtggcgacg actgacctga ccgtcgatga
     1081 catggtcgac atcggggtca tgctgctctt tcgcgccgac tacaacccgt acccggtttt
     1141 caccgaagtc gcgcgcacgc tcactccgta tcaaccgttc aaccgctgac agcatcacaa
     1201 ctgccgggcc gtcaggcggg aactccccac cagttccaga tttctctgac tgttcttcgc
     1261 ctcaacgcgt accggtcgaa cggcagaaac ctctggcgca aatggagtgc aaatcctagt
     1321 tgggtcgagg cgcaatttct gatagtatga acggtacttc atgccttctc gaggaatgat
     1381 ggaactcatt cgcgtctgca tcgtgaccga cgatcggaac acctttgaag cctgttccga
     1441 aatgctctcg tctttcagtc ccggggaatt caagatcgaa tggctcgccg ggtttgaaag
     1501 cgcgatcgaa gagttaaaag ctttttccgg tggcgtgttt ctcgtcgatg aattcctgaa
     1561 gaccctctcc tgctgggatc tcctcgccac cattcgtcag tatcccggca ttccccccat
     1621 catcgtcctc tcccatcccg cggctggtgc agacacgatt tcaccgaccg agcatctgtc
     1681 tctcgaagag cgggcgatgc gagaaggagc ctttgatctg ttgccaatgc cccagctcac
     1741 gccggtgctg ctggtcaaga tggtacgcta tgcgcaccgg cactcgcaca ccatcgccga
     1801 tctcgccacc agtgaagaac gatacgctct ggccatgaag ggcacgactg acggtatctg
     1861 ggactggaac ctggaaaccg atgaagtctt tttctcgagc cgctggaaga gcctgctcgg
     1921 tctggccgaa gacagcattg gcagccgccc cgacgaatgg tttcagcggg tccatcctgc
     1981 tgatgtgctg cgactgaaag ggcagctggc ttcacatctg gccggccaca cgccgcattt
     2041 cgagagtgag tatcgcgtcc tgcaccacga cggcacctac cgctgggtca tcagccgtgg
     2101 ggcctcggtt cgcggccccg atggcaaagc ctaccgcatg gtcgggtcac agaccgacat
     2161 ttcaggtcgc aagaaggtcg agcaggagat tctgcatcgc actgtgttcg agatgctgat
     2221 ccgcagcatc tccaccaagt tcatcaactg cccgtcgaaa cagattcgcg aagcgattgc
     2281 cgagagcctc aaatcgatcg gcga
//
LOCUS       DAORHD010000835         2302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57419_length_2302_cov_3.172675, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000835 DAORHD010000000
VERSION     DAORHD010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57419_length_2302_cov_3.172675"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKO06_16040"
     CDS             <1..404
                     /locus_tag="PKO06_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71215.1"
                     /translation="HGVNVPGKGGVIQVFLSTDPKTGEIRSFFFQRLESRAARLLKDR
                     KFRDGFAGLSLADFYKHDYFRSAEPESPADRLARLLPVVTDADQDADVRASVRGVRKN
                     LILLDFFLYDRRNEPFFERTRQLLSEKGKRL"
     gene            401..778
                     /locus_tag="PKO06_16045"
     CDS             401..778
                     /locus_tag="PKO06_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71216.1"
                     /translation="MKAIILLCVLILAFGTGYLAQRASAPEVFGRPFQIGEETHLESI
                     LGEPDRYMGTDVRLSGKIVRQCPSAGCWFFLEAAQGKRLRVELGHLGLKFPQRVGRVA
                     EVEGRLLRNRGEVELVGSTARFN"
     gene            799..2043
                     /locus_tag="PKO06_16050"
     CDS             799..2043
                     /locus_tag="PKO06_16050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71217.1"
                     /translation="MSFFRLALRNLWHRPLRTALTVVGVGLAVAVAVSLIGFNLGYRE
                     SINVSIDKLGFQVMLMAKGCPYEAATMMLKGGTGLLYLPGTTFSAVKADPELAAITPI
                     FVGVAQKEGSGIREDSGSSFAIVSGIDRSSFLAMKPWMTFSDRPGYQGGRWFADESSD
                     EVVMGFEAAEYEQRKVGDRLYLSITPQGQTEPVSHEFKVVGILERTGTQDDGTVFMSL
                     PLARRIFNRPDELTIVGVKLREFSGVALREFEKRWAALPEVQVVSLQQVKTTLVSLVE
                     TAQAMIGAVAAVAIIVAIIGVINTILMSVGERTGEIGIMKAIGAARGDIFQLIWLETL
                     LICVSGGVLGCAGAVLGSRGVEEALRRLIDVGTRGSIVSITVEVLAGALVGAAVVGVF
                     AGLYPAWRASSLRPVEAIREGE"
     gene            2045..>2302
                     /locus_tag="PKO06_16055"
     CDS             2045..>2302
                     /locus_tag="PKO06_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71218.1"
                     /translation="MNTPINQVCSTSQDPHLIEANNLKRWYTRGSETVKALDGVSLIV
                     PPGQLISILGPSGSGKTTLINLLSCLDAPDDGELKFAGRDVR"
BASE COUNT          437 a          660 c          726 g          479 t
ORIGIN      
        1 tgcatggagt gaatgtccca ggtaagggag gagtgatcca ggtgttcctc tccacggatc
       61 cgaagactgg tgagatccgc tccttctttt tccaacgact ggaaagccgc gccgcgcgcc
      121 tgctgaaaga ccgaaaattt cgtgacggct tcgccggcct cagtctggcc gacttctaca
      181 agcacgacta ttttcgatca gccgagcccg aaagcccggc agatcgactg gcccgtcttc
      241 ttccggtcgt cacagacgcc gaccaggatg ccgacgtacg cgcttccgtg cgcggggtcc
      301 gcaagaatct gattctgctc gatttctttc tgtacgaccg ccgaaacgag ccgtttttcg
      361 agcgaacccg acaactgctg tcggaaaagg ggaaaagatt atgaaagcga tcattctgct
      421 gtgcgtgttg atcctggcct tcggcacagg gtatctggct cagcgggcct cggcccccga
      481 ggtcttcggt cgtccattcc agatcggcga agaaactcat ctggagtcga ttctgggtga
      541 gcctgaccgg tacatgggga cggacgtgcg cctctcgggg aagatcgtcc gtcagtgccc
      601 cagcgcggga tgctggtttt tcctcgaggc ggctcagggc aagcgacttc gggtcgagct
      661 cgggcacctg ggcttgaagt ttcctcagcg ggtgggtcga gtcgccgagg tcgaaggtcg
      721 attgttgcga aaccgtggag aagttgaact ggtaggcagt actgcccgct tcaactagga
      781 cgtggaaagg acgaggatat gagcttcttt cgcctggctc ttcgcaatct gtggcaccgt
      841 cctctgcgaa ccgctctgac cgttgtcggg gtgggcctgg ccgtggcggt agccgtcagc
      901 ctgatcggtt tcaatctcgg ctatcgagaa tccatcaacg tcagtatcga caagctgggg
      961 ttccaggtga tgctcatggc caaggggtgc ccctacgagg cggcaaccat gatgctgaag
     1021 ggcggcaccg gcctcctcta cctgcccggc acgacgttct ccgcggtcaa ggcagaccct
     1081 gaactggccg ccatcacccc gatcttcgtc ggcgtcgccc agaaggaagg cagcggaatc
     1141 cgcgaggact ccggctcctc attcgccatt gtctccggaa tcgatcgatc cagttttctc
     1201 gccatgaaac cctggatgac gttttccgat cgtccgggat atcagggtgg gcgctggttt
     1261 gccgacgaat cctccgacga agtggtcatg ggattcgaag ctgcggaata cgagcaacgg
     1321 aaggtgggcg atcgtttgta cctgtcgatt accccgcagg gtcagaccga accagtttct
     1381 cacgaattca aggtggtcgg gattctcgaa cggaccggta cccaggatga tggaaccgtg
     1441 ttcatgtcgc tgccgttggc acgccggatc ttcaatcggc cggacgagtt gaccatcgtc
     1501 ggggtaaagc tcagggagtt ttcgggcgtg gccttgcgcg aattcgagaa gcgttgggcc
     1561 gctctgcccg aggtgcaggt agtcagtctg cagcaggtga agacgacgct ggtcagtctg
     1621 gtcgagaccg cccaggccat gatcggagcc gtcgccgcgg tggccatcat tgtggccatc
     1681 attggagtga tcaacacgat cctgatgagt gtcggcgagc gtacggggga aatcggcatc
     1741 atgaaggcta ttggagccgc tcgcggcgac atctttcagc tcatctggct tgaaacgctg
     1801 ctcatctgcg tcagtggggg agttctcgga tgtgccggcg cggttctcgg ctcgcggggg
     1861 gtggaagaag ccctgcgacg cctgatcgac gtcggcaccc ggggatcgat cgtctccatc
     1921 accgtcgaag tgctcgcggg ggccctggtc ggagctgcgg tggtcggagt gtttgccggc
     1981 ctgtatccag cctggcgcgc atcttcactg cggccggtgg aagcgattcg cgagggggaa
     2041 tgacatgaac acacccatca atcaagtctg ttcaacaagc caggaccctc atctcatcga
     2101 agccaacaac ctgaagcgct ggtacacaag aggaagcgaa acggtgaagg ccctcgacgg
     2161 cgtcagcctg atcgttcctc cgggtcagct gatctcgatt ctcggtccct ctggatcggg
     2221 caagacgacc ctgatcaacc ttctttcctg cctggacgca ccggacgacg gagagctgaa
     2281 attcgccgga cgcgacgtgc gc
//
LOCUS       DAORHD010000836         2300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57513_length_2300_cov_2.472606, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000836 DAORHD010000000
VERSION     DAORHD010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57513_length_2300_cov_2.472606"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2300)
                     /locus_tag="PKO06_16060"
     CDS             complement(<1..>2300)
                     /locus_tag="PKO06_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71219.1"
                     /translation="KLRDGTSGSEKVYESRATEHHDLPSLQGEISYTVSALDDAGNES
                     PTRRTASLSYDLTPPSVKGIFSPSFGRRLIPIYGLMSVTWKDIGEVVRPGPLDVALSF
                     DEPLREAPKLSWRTSIADVPQSITLSRKTDTYYTGRLELPRCPGQDVDIRFALEASDA
                     AGNKSQPLGEEARLLLDSDPPAAINQLLVEPGPLGSVRCSWAPPGKEPEGEPMYRLYR
                     SNSSFNSASQAELIIDKQRETKLTDRPPQDGTWYYSVAAVDRAGYVGSLTPAISVMAD
                     SQPPAAPASLGVLLGREVVLSWTQANGSDTADWEVYLASEAIASLSDLQKHKPAAEHL
                     TEPRYAFVPPSWPTIHLAVVARDQAGNRALSSLAPPLNISDKLPAAHLRIEPPSPARG
                     AVRVLIESTLPLKSVPSLWFEPQGRSPIPLGVASDPASARSFTAALAVDERTGDGTGT
                     FRFLGYSLDDLTGTNILTGKHLLIDTRAPRLRIDFVSGVPMRAGPVAVRVLADEPLAA
                     PPEVTLIPQGGSPQTIALALATDGAYIGETSLAATLPDGLASVKASGRDLAGNVTSSI
                     DPDRVFVDNHAPQAPTTLVAVARRHGTVELRWQAPASAFGEEHLREYVLYRHDSSFTH
                     AASATVAARVRHPSCIDSTIPRDGLWYYAVAAIDEAGWSSPLSQIVSVVSDRTPPPAP
                     LPPTISMVGDVIQLQWQSVARNASEAITYEVTCESPDPLGGRNLVARIFGPATATTTT
                     HRPVFGGDLLYRVRGVDPLGHESDWS"
BASE COUNT          475 a          645 c          755 g          425 t
ORIGIN      
        1 aagaccagtc actttcatga ccgagcggat caaccccacg cactcgatac agcagatcgc
       61 cgccaaacac gggacgatgc gtcgtggtcg tggcggttgc cggaccgaag atgcgggcga
      121 caagattccg gccgccgagc ggatctgggg actcgcaggt cacttcatag gtgatcgcct
      181 cggaagcatt tctcgcgacc gactgccact gcagctgtat cacatcaccg accatcgata
      241 tcgttggcgg cagaggagca gggggtgggg tccggtctga aaccacagaa acgatctggc
      301 tcaaggggct tgaccaccct gcctcgtcga tcgcggccac ggcgtaatac cagagaccgt
      361 cacggggaat tgtcgagtcg atgcaggagg ggtgtcgaac gcgtgcggcg accgtggctg
      421 atgctgcgtg cgtaaacgaa ctatcgtgac gatagaggac atattcgcgc aggtgttcct
      481 ctccgaaagc cgatgccggt gcctgccaac gcagttcgac ggttccgtgt cgacgcgcga
      541 cagcgaccag tgtggtgggc gcctggggcg cgtgattgtc gacgaacacg cggtcaggat
      601 cgatgctgga agtcacattc ccggcaaggt cacgacccga tgctttcacg gatgcgaggc
      661 catcgggaag ggtcgcagcg agggacgttt ccccgatata cgctccgtcg gtggccaatg
      721 ccagggcaat cgtctgagga gatccaccct gcggaatcag ggtcacctca ggcggagccg
      781 ccagaggctc atctgccagc acgcgaaccg caaccggccc ggcgcgcatc ggaactccgg
      841 agacaaaatc gatgcgcagg cgcggggctc gggtgtcgat cagcagatgc ttgccggtga
      901 gaatattcgt accggtcaga tcgtcgagag aatagccgag aaagcggaaa gtgcctgtgc
      961 catctccagt tctttcgtcg accgccagag cggcggtaaa ggaacgcgcg gaagcggggt
     1021 cggaagccac gcccagtgga atgggcgatc gtccctgtgg ttcaaaccag agggatggca
     1081 cgctcttcaa cggcagggtt gactcgatga gaactcgtac tgctccgcgc gccggagaag
     1141 gaggttcgat cctcagatga gccgccggaa gcttgtcgct gatgttcagc ggcggtgcga
     1201 gagacgagag ggcgcgattc cctgcctgat cacgggccac gacggccaga tggatggtgg
     1261 gccaggacgg aggaacaaag gcatagcggg gttcggtcag atgctcagcc gctggcttgt
     1321 gtttctgtag atcggaaaga gaggcgatcg cttccgaagc cagatagacc tcccagtcgg
     1381 cggtgtcgga gccattcgcc tgcgtccagg agagtacgac ttcgcgcccg aggaggactc
     1441 ccagcgaggc gggcgcagca ggaggttgtg aatcggccat gacggatatc gccggtgtca
     1501 gcgaaccgac gtagccggcg cggtcgacgg cagcgacgga gtaataccac gttccgtcct
     1561 gagggggacg gtcagtcagt ttcgtctcac gctgcttgtc aataatcagt tctgcctgag
     1621 atgcactgtt aaaacttgaa ttcgatcgat acagcctgta catgggttcg ccttccggct
     1681 cttttcccgg tggagcccat gaacaccgaa cactgcccag cggaccgggt tcgaccagca
     1741 gttgattgat tgccgcggga ggatcggagt cgagcagcag acgagcttct tcccccagcg
     1801 gctgagattt gttgccggcg gcatcagagg cctcgagggc aaagcggata tcgacatcct
     1861 gcccgggaca tcgggggagt tcgagacgtc cggtatagta ggtgtcggtt ttccggctca
     1921 gagtgatcga ctgaggcacg tcggcgatac tggttcgcca ggacagcttc ggtgcctctc
     1981 ggagaggttc gtcaaaacta agagcgacat caagcggccc agggcggacc acttcgccga
     2041 tgtctttcca ggtgacgctc atcaatccgt agatcggtat cagacggcgc ccgaaggatg
     2101 gcgagaagat ccccttcacg ctcggtgggg tcaggtcata ggagagactc gcggtacgcc
     2161 tagtcggact ttcgttcccg gcatcgtcga gcgcgctgac cgtgtaagag atctctccct
     2221 gaagcgatgg cagatcatga tgctctgttg cgcgactctc atataccttt tctgagccag
     2281 aagtgccgtc cctgagcttg
//
LOCUS       DAORHD010000837         2299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57534_length_2299_cov_3.459893, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000837 DAORHD010000000
VERSION     DAORHD010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57534_length_2299_cov_3.459893"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PKO06_16065"
     CDS             complement(<1..320)
                     /locus_tag="PKO06_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020558459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HNV71220.1"
                     /translation="MNHHPVKSPRKLIEVALPLDDINVAAAREKSIRHGHPSTLHLWW
                     ARRPLAAARAVIFAQMVNDPGYERHLQRGMNKEKAAVERERLFRIIRDLVQWENTTNE
                     RVLE"
     gene            complement(317..886)
                     /locus_tag="PKO06_16070"
     CDS             complement(317..886)
                     /locus_tag="PKO06_16070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02646 family protein"
                     /protein_id="HNV71221.1"
                     /translation="MVELEHTHEPAELKRFRNGNPSATWNSEDFNQVRTIIREQLHHE
                     QDGLCVYCECKIDSNVGHIDHIKPRASHPQLAFIFENMAVSCSARDHCGHIKADKTLP
                     IEPTVGCNQFFSLAASTGLLFPAPEQPAGDQEAARQTINILGLNHSPALVRYRLQFLN
                     TLRGLPNPEDRQEFLSTSPFRWSLKGVLP"
     gene            complement(879..2252)
                     /locus_tag="PKO06_16075"
     CDS             complement(879..2252)
                     /locus_tag="PKO06_16075"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71222.1"
                     /translation="MLSTLDTFTNTRIEHVEVRNFRCFDHLSVAFHKRLTVLVAPNGG
                     GKTALLDLLALSLRPYLDNLQLKITKRIFRRQDVRQVLGPAGTMESVPQIFVQSKGLF
                     SKHEVRWALSSARGATTSLKGANDLQVAANTLFGQHLRSLGGEPAPVFPLFGYYGTGR
                     LWAHRRQASAPKARAQKPLDRPSGYDDCLSPDSRYEAFVDWFDRYSHEAAKEHMTNDP
                     SLHHPKDRLSAITQAVDLALEPSGWKNMEWDFTLKTIFARHQRLGRLPVSSLSDGIRN
                     MIGLVADIAHRAVRLNPQFGADAARRTPGIVLIDEVDMHLHPEWQQGVVSSLLNAFPE
                     IQFIVTTHSPQVLTSLPKTVIRILAQNNEGAWCADMPEISPLAQESGDALAFIMNTHP
                     RPGMPEIEADLHAYEILARQGKSSSPEALAIKAKLEAAGYEFSQAQRALFDFLTGRNR
                     GGGHGNG"
BASE COUNT          490 a          636 c          654 g          519 t
ORIGIN      
        1 tcttcgagca cccgctcgtt ggtcgtgttt tcccattgta cgaggtcgcg aatgatgcga
       61 aacagtcgtt cacgctcgac ggcggccttt tctttgttca tgccgcgctg aagatgccgc
      121 tcataccccg ggtcattcac catctgcgcg aaaatcaccg cccgggctgc cgccaggggc
      181 cgccgtgccc accaaagatg gagggtcgac ggatgcccgt gccgaatcga cttctcgcga
      241 gccgccgcca cattgatatc atcgagcggc aacgccacct caatcagctt gcgcggtgat
      301 ttcacggggt gatgattcac ggtaggactc ctttgagtga ccagcggaat ggagacgtcg
      361 acaggaactc ctgccggtct tccgggttcg ggagaccccg gagggtgttg aggaattgaa
      421 gacggtaacg aaccaatgcc ggtgagtgat ttaaccccag gatgttgatg gtttgacgcg
      481 ccgcttcctg gtcacctgcc ggttgttctg gtgcagggaa cagcaacccg gtagatgcgg
      541 caagagaaaa aaactggttg catccgacag tcggctcgat tgggagcgtc ttgtcggcct
      601 ttatgtgacc gcaatgatct ctggcggaac aactcacggc catgttctcg aagataaagg
      661 ccagttgcgg gtgtgaggct ctcggcttga tgtgatcgat atgcccgaca ttcgagtcga
      721 ttttacactc gcaatagacg caaagcccat cctgctcatg gtgcagttgc tcgcgaatga
      781 tcgtgcgaac ctggttgaaa tcctccgaat tccaggtcgc gcttggattt ccgtttcgaa
      841 accgctttag ctcggcaggt tcgtgggtat gctccagctc aaccattccc gtgacccccg
      901 cccctgttcc tgcctgtgag aaaatcgaac agggctctct gcgcctgcga aaattcgtac
      961 ccggcggctt cgagcttcgc cttgatggca agagcttcag gcgaactgct ttttccctgt
     1021 cttgccagta tctcgtatgc atgcaggtct gcttcgatct cgggcatacc tggccgtgga
     1081 tgggtattca taatgaatgc cagtgcgtca cctgattcct gagccagagg gctgatttca
     1141 ggcatgtcgg cgcaccaggc accttcgtta ttctgagcca gaattcgaat gaccgtcttc
     1201 ggcagagagg tcagaacctg cggactgtgc gtcgtcacga tgaactggat ttcgggaaag
     1261 gcgttcaaca gagacgagac aaccccctgc tgccactcag ggtgcagatg catatcgacc
     1321 tcatcaatca gaacaatgcc gggtgttcgg cgagccgcat cagcaccaaa ctgcggattg
     1381 aggcgaaccg ctcggtgggc gatgtcggcg accagaccga tcatgtttcg aatgccgtcg
     1441 ctcaatgaac tgacaggcaa ccgccccagt cgctggtgac gtgcaaaaat ggttttcaaa
     1501 gtgaagtccc attccatgtt tttccagccg cttggttcca gcgccaggtc aacagcctgt
     1561 gtgatcgctg acaatcgatc tttcggatgg tgaagcgacg gatcgtttgt catgtgttct
     1621 ttcgcggcct catgagagta ccggtcaaac caatcgacaa acgcctcata ccgcgaatct
     1681 ggcgagagac aatcatcata accgctgggt cgatccaggg gcttctgcgc gcgcgccttc
     1741 ggagcacttg cttggcggcg atgtgcccac aaacggccag tgccgtaata tccgaagagc
     1801 ggaaacaccg gagccggttc accgcccaga gatcgaagat gctggccaaa aagcgtgttg
     1861 gcggctacct gaagatcgtt tgctcccttg agtgaggttg tcgccccacg cgcgcttgaa
     1921 agagcccacc gaacctcatg cttactgaac aaccccttcg actggacgaa gatctgtgga
     1981 acggactcca tcgttccggc aggcccgaga acctgcctca catcctgccg ccgaaaaatc
     2041 cgtttcgtta ttttcagttg gaggttgtcc aagtatggtc tcagagagag agcgagcaag
     2101 tcgagaagcg cggttttgcc gccgccgttg ggggcgacca ggacggtcag acgcttgtga
     2161 aaggcgacgc tcagatggtc gaagcaacgg aagtttcgca cttcaacgtg ttcaatgcgc
     2221 gtgttggtga aggtgtcgag cgttgataac atttcgttac ttcccgatgg cgttactaaa
     2281 ggataatagc acattcgtt
//
LOCUS       DAORHD010000838         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57563_length_2298_cov_3.998217, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000838 DAORHD010000000
VERSION     DAORHD010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57563_length_2298_cov_3.998217"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PKO06_16080"
     CDS             complement(<1..812)
                     /locus_tag="PKO06_16080"
                     /inference="COORDINATES: protein motif:HMM:NF015556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71223.1"
                     /translation="MSLRQRIHYWYRLLHRYNPFRAEVGLRRIGQPDEQAPVLVSGNY
                     EHTVEQLEEALQGQNCWLLVADSAGINVWCAAGVGDFNENKIIDAVVATELDRQVRHR
                     RLILPPLAAVGIDVKRLMRETGFTVVWGPTHLDDLPDFLRAGNKRTDAMRLARFPWRD
                     RWENAVGVIGVFLFPILLIRRFPRQIGWYFFSLLHAVCGSLLLYDRLPPKYPANKTVL
                     LGTAQAVMLWLSHRLRRRDPHLLERLLIGAGVHLLIAIDMIGSTPFYKSTMV"
     gene            complement(809..1507)
                     /locus_tag="PKO06_16085"
     CDS             complement(809..1507)
                     /locus_tag="PKO06_16085"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV71224.1"
                     /translation="MIIYGLMKFIEQIPERYDRMIGWLTLGGLQTARQVMLADVRPGM
                     RVLDVGCGTGSFLLEAAGRGAICVGVDASLPMLEVFRRKLASTSPEIRDRITIVHQSA
                     SLLNRAATPGSFDLVTSSLMLGELPTIVLDAVLRQIPEMLIPGGKVLICDELWPRERW
                     RSLAYSFVLAVTFVPNFILTRTMIRPVKGLEERMERHGLRVTGRTDFAWGVVSVVAAE
                     PCQPNHEARRGPTT"
     gene            complement(1504..2106)
                     /locus_tag="PKO06_16090"
     CDS             complement(1504..2106)
                     /locus_tag="PKO06_16090"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV71225.1"
                     /translation="MIRNVIITGIGGQGVVTAASLVRWAALDCGYVVRGLDNRGGAQR
                     LGHVSAVIRCCREPDRPVSPEIPRGECDLLLSLEAGEGLRFLPFLAKNAVVVHATRLV
                     VPTNQRRRRLDYLTLDQCRTGYSTCSSRVCEIDLDREAWRRFRKPVLANLIALGAGLQ
                     MAGWSDFAASLTPTLAEESRAAFEVGVSLARSRCGEKERA"
     gene            complement(2103..>2298)
                     /locus_tag="PKO06_16095"
     CDS             complement(2103..>2298)
                     /locus_tag="PKO06_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV71226.1"
                     /translation="FVLDVDRCHECLLCYERFCCPAIVKNADGKLEIDPVLCSRCGVC
                     EQVCPNGAIASVQIREDPKP"
BASE COUNT          498 a          751 c          666 g          383 t
ORIGIN      
        1 tgcaccatgg tactcttgta gaacggcgtc gaaccgatca tgtcgatggc gatcagcaga
       61 tgcacccccg ctccgatgag cagacgttcg agcagatgcg gatcgcgacg acgcaggcga
      121 tggctcaacc acagcatcac cgcttgtgcc gttcccagca gaaccgtttt gttggcggga
      181 tatttcggcg gcaggcgatc atacagcagc agactgccgc acaccgcgtg caagagcgag
      241 aaaaagtacc agccaatctg ccgcggaaat cgtctgatga gcaggatcgg gaagagaaat
      301 accccgatca cgccgacggc gttttcccac cggtcgcgcc agggaaaccg ggccagtcgc
      361 atggcatccg tgcgtttgtt ccccgcacgc agaaaatcgg gcagatcgtc caggtgagtc
      421 ggaccccaga cgaccgtgaa tcccgtttct cgcatcaatc gtttcacatc gatgccgaca
      481 gccgccagag gcggcagaat caagcgtcgg tgtcgaacct gccggtcgag ttccgtcgcc
      541 acgacggcat cgatgatctt gttctcgttg aaatcgccga ccccggccgc gcaccagacg
      601 ttgatccccg ccgagtcagc cacgagcagc cagcaattct gcccctgcag cgcttcttcg
      661 agctgttcca cggtgtgctc gtagtttccc gataccagca ccggagcctg ttcatcaggc
      721 tgaccgatgc gtcgcagccc gacttcggcc cgaaacggat tgtaccgatg cagcaggcgg
      781 taccaataat gaattcgttg tctgagactc acgtcgtcgg gcctcgacgc gcttcgtggt
      841 tcggttgaca cggctctgcc gccacgaccg agacgacgcc ccaggcgaaa tccgtacgac
      901 cggtcacccg caggccatgg cgctccatgc gctcttcaag ccccttcacg gggcgaatca
      961 tggttcgcgt cagaatgaaa ttcggcacga aggtcacggc cagcacgaaa gaataggcca
     1021 gactgcgcca gcgctcccgc ggccacaact cgtcgcagat caataccttg ccgccgggaa
     1081 tcagcatctc gggaatctga cgcagaacgg catccaggac gatcgtcggc agttcaccga
     1141 gcatgagcga tgaggtcaca aggtcaaacg aaccgggggt cgcggcgcga ttcaacaggg
     1201 aagcgctctg atgcacgatc gtgatgcgat cgcggatctc cggtgaggtc gacgccagct
     1261 tgcgacgaaa aacctcgagc atgggcagag acgcatctac cccgacacag atggccccgc
     1321 ggcccgccgc ctcgagtaaa aagctcccgg taccgcaacc gacgtcgagc acccgcattc
     1381 cgggtcgaac gtcggccagc atgacctggc gagcggtctg cagcccaccc agggtcagcc
     1441 acccaatcat gcgatcgtag cgctcgggaa tctgttcgat gaacttcatc agtccgtaga
     1501 tgatcatgcg cgctcctttt cgccgcagcg gcttcgcgcc agcgacaccc ccacctcgaa
     1561 cgcggcacgc gactcttcgg ccagcgtcgg cgtcagcgaa gccgcgaagt cagaccatcc
     1621 ggccatctgc aggccggcac ccagagcgat cagattcgcc agcaccggtt tgcgaaacct
     1681 gcgccaggct tctcgatcga gatcgatttc acagactcgc gaactacagg tggaataccc
     1741 ggtccgacac tggtccaggg tcaggtagtc caaccggcgc cggcgctggt tggtgggaac
     1801 caccagccgg gtcgcatgca cgaccacggc attctttgcc aggaacggca ggaagcgaag
     1861 cccttctccg gcttcgaggc tcaggagcag atcgcactca ccgcgaggaa tttcgggcga
     1921 gaccggtcga tcgggttcac gacagcagcg aatgaccgcg gacacgtgtc ccaggcgctg
     1981 cgctccgccg cgattgtcga gaccgcgtac gacatagccg caatcgagag cggcccatcg
     2041 caccagagaa gccgcggtga ccaccccctg cccaccgatg ccggtgatga tgacgttacg
     2101 gatcatggct tcggatcctc gcgaatctgg acagacgcaa tggccccgtt cggacagacc
     2161 tgttcacaga ctccgcatcg tgagcaaagc accgggtcga tctcgagctt tccatctgcg
     2221 ttcttgacga tcgccggaca gcagaaacgc tcatagcaca gcagacattc gtgacagcga
     2281 tcgacatcga gaacgaac
//
LOCUS       DAORHD010000839         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57578_length_2298_cov_2.993313, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000839 DAORHD010000000
VERSION     DAORHD010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57578_length_2298_cov_2.993313"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PKO06_16100"
     CDS             complement(<1..205)
                     /locus_tag="PKO06_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71227.1"
                     /translation="MSHAAPFMSMRWLRLHIKEIIWATVILFVLSCFIIGYGSSRAQR
                     RMEERRTEAEAAERRAQETEESLP"
     gene            414..>2298
                     /locus_tag="PKO06_16105"
     CDS             414..>2298
                     /locus_tag="PKO06_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNV71228.1"
                     /translation="MCGVIGCVFEKDTSTLGQTATRLLRMLEYRGYDSTGGIVQDSDG
                     RITLRKDVGAPSDVAVRLGIDRLSGHIFCGQVRWATFGVVSRDNAQPHEMTCKLHFYG
                     AHNGNITNCERLKEWLKREGHAVKSDNDGEMLVHTIEHYFAEELRYKDADDREVRHEA
                     LRQAVLRASTQVVGSYASVVVDPTSGLMAVIKAGSSLYLGRGVHPDGGAFHLASSDLA
                     SVLQLTRVLIPIREKEFAIFDHQQFTLYDLRNGRKIKRPSQRSLLKVEETRLDPAFTY
                     FMEQEIHHQVPSARRLIERFDGGDALVARVRQKKVDRDLLAKCRTEVARLAEVTETEE
                     LRAGWRKWLESAPARSLEHLAGEVEGLTQGRRLESTHGAFLEELREISDCARFPTLSP
                     MLACLDAAFDLSEVEDLEKRLRRFIQIMKQARERNGTIYILACGSSFHAGKTAPLFFN
                     EIAGVPVIPLLPGEFRAQCTQSLRAEDVLIGISQSGETKDLIDVFNFVTENYPGVKKI
                     CVLNNTNSTLAQEKSDLYISLYCGPEISVPATKSFMNQLLVLFMLAVRLREELQPKGR
                     RGARKTRAGEADFWAMVERVPDLIDRTIKTTKKEIEEIANELFIRPSIHILATRLLGI
                     AKEG"
BASE COUNT          493 a          646 c          700 g          459 t
ORIGIN      
        1 ccggcagtga ttcttcggtt tcctgggccc gacgttcggc ggcttcggct tcagtacgac
       61 gttcctccat gcggcgctgg gcgcgcgagg aaccgtatcc gatgatgaag caggagagca
      121 cgaacagaat caccgttgcc cagatgattt ccttgatatg caggcgcaac cagcgcatgg
      181 acatgaatgg ggcagcgtgg gacatggtta tgaactctcc tcgttgctga gattgtttcc
      241 gtttctgcgg tgatttccga atagggaaac cccccgtctg atggggggaa agcgcggcga
      301 ttatagcacc ggggtaccgg gaagtcaaaa ctttctcccg gttgcactct cccggtgtcg
      361 ggaatacaat tcacgccgaa acgacccctg aaaaagacgg aaaggaatca tctatgtgcg
      421 gagtcatcgg ctgcgttttt gaaaaagaca cctcaaccct cggacagacg gcgaccagac
      481 tgctgcgaat gctcgagtat cgcggctacg attcgaccgg tggcattgtc caggatagcg
      541 atggccgaat cacgctgcgc aaggatgttg gcgctcccag cgatgtggcc gtgcgtctgg
      601 gtatcgacag actgagcggg cacatcttct gcggccaggt gcgctgggcg acatttggcg
      661 tggtcagccg cgacaacgcg caaccccacg agatgacctg caaactgcat ttctatgggg
      721 cgcataacgg gaatatcacg aactgtgaac gactcaagga gtggctgaag cgtgaagggc
      781 acgcggtgaa gagtgacaat gacggcgaga tgctcgttca caccatcgaa cactattttg
      841 ccgaagagtt gcgttacaaa gatgccgatg accgcgaggt ccggcatgaa gccttgcgcc
      901 aggcggttct gcgggcgtca actcaggtcg tggggtcgta tgcctcggtc gtggtcgatc
      961 cgacctccgg gctgatggca gtgatcaaag cgggaagcag tctgtatctg gggcgtgggg
     1021 tgcatcccga cggcggagcg ttccatctcg cctcgtctga tctggcatcc gtactgcaac
     1081 tgacccgggt tctgattccc attcgcgaaa aagagtttgc catcttcgat caccagcagt
     1141 tcaccttgta cgatctgcgc aacggccgca agatcaagcg gccttcgcag cgcagcctgc
     1201 tcaaggtcga agagacccgt ctcgaccctg cgttcaccta cttcatggaa caggagatcc
     1261 atcatcaggt gccttctgcg cgccgtctga tcgaacgttt cgacggcggc gatgccctgg
     1321 tggcgcgggt gcggcagaaa aaagtcgatc gcgacctgct cgcgaagtgc cgcaccgagg
     1381 ttgccagact ggccgaagtg actgaaaccg aggagttgcg cgcgggctgg cgcaagtggc
     1441 tggaatcggc gccggcgcga tcgctcgaac atctcgcggg agaggtcgag gggctgactc
     1501 aggggcgtcg cctcgaatcg acccacggag cctttctcga agagctgcgc gagatctctg
     1561 attgtgcccg ttttccgacg ctttcgccga tgctggcctg tctcgatgcg gccttcgatc
     1621 tgagcgaggt cgaagatctc gaaaagcgat tgcgccgatt catccagatc atgaagcagg
     1681 cccgtgagcg caacggcaca atctacattc tggcctgcgg cagctcgttc cacgcaggga
     1741 aaaccgcgcc cctcttcttt aacgaaatcg cgggcgtgcc ggtcattccg ttgctgcccg
     1801 gagagtttcg cgcccaatgc acgcagtccc tgcgggccga agacgtgttg atcggcatca
     1861 gccagagcgg cgaaaccaag gacctgatcg atgtcttcaa cttcgtgacc gagaattatc
     1921 ccggcgtgaa aaagatctgc gtcctcaaca acacaaattc gacgctggct caggagaaat
     1981 ctgacttgta catttctctc tactgcgggc ccgaaatatc ggtgccggca accaagagct
     2041 tcatgaacca acttctggtg ctcttcatgc tggcggtccg tctgcgagag gaactgcagc
     2101 ccaaaggccg gcgcggggct cgcaagactc gcgcgggaga agcggatttc tgggcgatgg
     2161 tcgagagagt gcccgatctc attgatcgca cgatcaaaac caccaagaaa gagatcgagg
     2221 aaattgcgaa cgagctgttc attcgaccga gtattcacat tctggcgacg cgcctgctcg
     2281 gcattgccaa agagggcg
//
LOCUS       DAORHD010000840         2297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57626_length_2297_cov_2.931311, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000840 DAORHD010000000
VERSION     DAORHD010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57626_length_2297_cov_2.931311"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKO06_16110"
     CDS             complement(<1..961)
                     /locus_tag="PKO06_16110"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV71229.1"
                     /translation="MPGSRIVQTVRHWIYRNAYLLTLLAPLFLLASVELWARAGGGGS
                     YSSGSRSYGSSRSGGRSGGGGGGEDIIIRLLIELVIRYPAVGIPVVIGVCVFVYYSSK
                     EGKEVYVDYTIRKGMRAQKDSLRQAALQALKKRDPQFTEDAFLKRVERAFLKVQKAWC
                     DHNLPLAQAFLSDGVLERFGLQIKEQIDKGVRDRMENIQVLERRVAQIQSDAHFDTIH
                     VYVRASAIDYQEDLKTKKPVGAKKGDEEFEEYWSFLRKPSAKSLSVKGGLIEGVCPNC
                     GAPLEVSATVKCPYCQALLRSGEYDWVLAEITQACEWAVQETRQ"
     gene            1119..1538
                     /locus_tag="PKO06_16115"
     CDS             1119..1538
                     /locus_tag="PKO06_16115"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNV71230.1"
                     /translation="MGIDNLIKTVLSELQVAINSKTVIGEPIAAQNWTIIPVTKVSFG
                     FGAGGGEGKKEANSFGGGSGGGATIEPMAFIAVNPKEVKLFSFKGKETVWEKILNVET
                     VEKIYQKLMGSGDDKTATTAESPATSGQEPPKTGPVA"
     gene            1583..>2297
                     /locus_tag="PKO06_16120"
     CDS             1583..>2297
                     /locus_tag="PKO06_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV71231.1"
                     /translation="MSVDVLKKLSLFEGLEDGDLTKINSVIKERTFPKGTVIFKEGDS
                     GDAFYLILDGGVEVIKKDGGEEKVAHTIEHTDKTPFFGEMALIEGVPRSATLRASKDL
                     KCMVIEKNDFDMMLRLNSFISLRIMTALSRRLRSSSAAEKPAETKQGQVIALFSPKSG
                     SGKSILAANLAAGLTKITGGKTLLIDLDLQFGDLGFMLPLKPKHTIADLVEKTANKCQ
                     DIKDFVVDHALGFSVLPAPK"
BASE COUNT          521 a          689 c          638 g          449 t
ORIGIN      
        1 cctgccgcgt ttcctgcacc gcccactcgc aggcttgggt gatctcagcc agtacccaat
       61 catactcgcc cgatcgcagc aatgcctggc agtacgggca cttcacggtg gcgctgactt
      121 cgagcggcgc accacagttc ggacacacgc cttcaatcag gccacccttc acagaaagtg
      181 atttggcgct cggtttgcgc agaaatgacc agtactcttc gaactcctcg tctccctttt
      241 tcgcgcccac cggtttcttg gtcttcaggt cttcctgata atcgatggcg gatgctcgca
      301 cgtagacgtg gatggtatcg aagtgcgcgt cgctttgaat ctgcgcgacc cgacgttcga
      361 gcacctgaat gttttccatg cgatcgcgca cacccttatc gatctgctcc ttgatctgca
      421 ggccgaagcg ttcgaggacg ccatcagaga gaaatgcctg ggccagcggc aggttgtgat
      481 cgcaccacgc cttctgcacc ttcaggaatg cccgttcgac gcgcttcaag aaagcgtctt
      541 cggtaaactg tgggtctcgt ttcttcaacg cctgcaaagc cgcctggcgc agactgtctt
      601 tctgggcgcg catgcctttg cggatcgtgt agtcgacgta gacttccttg ccctctttcg
      661 acgagtagta gacgaacacg cagacaccga tcaccacggg aataccgacc gccgggtagc
      721 gaatgaccag ctcgatgagc agacgaatga tgatatcttc gccaccaccg ccgccgccgc
      781 tgcgcccacc gctgcgactg ctgccatagc tgcgacttcc tgaactgtaa ctgccaccgc
      841 ccccggcccg ggcccacaac tcaacactgg ccagcagaaa aagtggcgcg agcagcgtca
      901 gcaggtacgc gttgcggtag atccagtgac gaacggtctg aacgatccgg gatcccggca
      961 tggtctttcc cccggctgat tggtgtcgtg ataccagcat agcatagccc gtcagagccc
     1021 ccaagcccgg ttgggaaaaa aacccgatac gcgggagtag ccttccggaa aaaagattgt
     1081 taccatagag cgaattttct ccagaggagc aagaagccat gggaatcgac aatctcatca
     1141 agaccgtgct gtctgagctg caggtggcca tcaattcgaa gacggtcatt ggcgaaccca
     1201 ttgccgcgca gaactggacc atcatccccg tcaccaaggt gtcgtttggt tttggcgccg
     1261 gcggcggtga agggaaaaag gaagccaaca gcttcggcgg cggctcaggc ggtggggcga
     1321 ccatcgagcc gatggccttt atcgccgtca atcccaaaga agtgaagctg ttctcgttca
     1381 aaggcaaaga gacggtctgg gaaaagattc tcaacgtgga aaccgtcgag aagatctacc
     1441 agaagcttat gggcagcggc gatgacaaga ccgccaccac ggcggaatca cccgccacca
     1501 gcggacagga gccgcccaag accggcccgg tcgcctgagg caccggcgtt cacgatcgaa
     1561 tccatcgagg ggagaatcag tcatgtccgt agatgttttg aagaaactca gcctgtttga
     1621 aggcctggaa gatggtgatc tgaccaagat caactcggtc atcaaggagc gcacgtttcc
     1681 caaaggaacg gtcatcttca aggaaggcga ttccggggat gcgttctacc tgattctcga
     1741 tggcggcgtc gaagtcatca agaaagacgg tggtgaagag aaagtcgcgc acaccatcga
     1801 acataccgac aaaaccccgt tttttggcga gatggcgctg atcgagggtg ttccccggtc
     1861 ggcaacgctg cgggccagca aagatctcaa gtgcatggtc atcgaaaaga atgatttcga
     1921 catgatgctg cgcttgaact ccttcatttc gctacgcatc atgaccgctc tgtcgcgacg
     1981 cctgcgctcg agcagcgccg ccgagaaacc ggccgaaacc aagcagggcc aggtgatcgc
     2041 cctgttcagt ccgaagagcg gttcaggcaa aagtattctg gccgccaatc tcgccgccgg
     2101 tctcaccaag atcactggtg gcaagacgct gctcatcgac ctcgatctgc agttcggcga
     2161 tctcggattc atgctgcccc tcaaacccaa gcacacgatc gccgacctgg tcgagaagac
     2221 cgcgaacaag tgccaggata tcaaggactt cgtggtcgat cacgccctcg ggttctcggt
     2281 gctgccagct cccaaga
//
LOCUS       DAORHD010000841         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57657_length_2296_cov_3.362784, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000841 DAORHD010000000
VERSION     DAORHD010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57657_length_2296_cov_3.362784"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PKO06_16125"
     CDS             complement(<1..675)
                     /locus_tag="PKO06_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71232.1"
                     /translation="MHTRLRVSVSGLFLTCLLAASLFLTACGNKPVAKPEAPAPKIEV
                     FTAQKGLPNSDITALAVFGGQIWAGTRNGLTRYDGVNWQIHVKKNTNALPSNEIEGLV
                     VTPDSLLIATENGAARYQAAGFSSIVTGLRVRSIAQNGPTVILATAHGIEQSTGGPFQ
                     PYGKENASLVDDEVNVVAFDRKGRVWAGTRVGMGLFQGTLFQNYSGPAKTIMGSSLVE
                     IPPSPAN"
     gene            834..1769
                     /locus_tag="PKO06_16130"
     CDS             834..1769
                     /locus_tag="PKO06_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71233.1"
                     /translation="MKKRLKTYPMRERSNLVKLADFSRLPEPGFRLTPFLDGFPQIHV
                     GKTFRRMVQAIVAARRAERPVILGMGAHVIKCGLSPWLIELLRRRVVTAIALNGAGSI
                     HDFELAFCGETSEDVARELAAGRFGCVEETGAWMGEAMRAAHRQGLGLGEGLYQAMKA
                     CPERYPHREISLLWQTGELGVPCTVHVAVGTDFIHLHEACDGAALGATTFRDFEIFCD
                     QVEQLEGGVYWNLGSAVVLPEVFLKAVSRAHNLGHHLAGMTTLNMDMLVQYRSMTNVV
                     KRPSLGVGDGFHLTGHHELMVPLLAQAVLEGLDAC"
     gene            1763..>2296
                     /locus_tag="PKO06_16135"
     CDS             1763..>2296
                     /locus_tag="PKO06_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV71234.1"
                     /translation="MLKRTRLQEILQSFTRLELAVLGDVMLDEFLWGKVDRISPEAPV
                     PIVQVKNETSRPGGAANVAANIVSLGARAHIVGVVGDDGAGRKLRESLESEKIATDGL
                     VIDPERRTTLKTRIIGHKQQMIRIDRESSGGIGEACGELIHAAQRGAGGIQRGESEIE
                     RGAIMGGQQLVAHFAAGV"
BASE COUNT          443 a          633 c          753 g          467 t
ORIGIN      
        1 gttggcaggt gagggcggaa tctcgaccag agaactgccc atgatggtct ttgcaggccc
       61 tgaatagttc tggaacagcg tgccctgaaa caaacccatc ccgacgcggg ttccggccca
      121 gacccgacct ttgcggtcga acgccaccac gttcacttcg tcatcaacca gcgaggcatt
      181 ttccttgcca tagggttgaa acgggccacc ggttgattgt tcgatgccgt gagcggtggc
      241 gagaatgacg gtcggaccat tctgggcgat cgagcgcacg cgcagcccgg tgacgatcga
      301 agagaaaccc gcggcctgat accgcgccgc cccgttttcc gtcgcgatca gcagcgaatc
      361 tggcgtgacc accagccctt cgatttcgtt cgagggaagg gcgttcgtgt tctttttgac
      421 gtgaatctgc cagttgaccc cgtcgtagcg ggtcagcccg ttgcgcgtgc cggcccagat
      481 ctggccgccg aaaacggcga gggccgtgat gtcactattg gggaggccct tctgtgcggt
      541 gaagacttcg atctttggcg ccggagcctc gggtttggcg accggtttgt tgccgcacgc
      601 agtcaagaac agagaagccg cgagcagaca ggtcagaaag agcccggaaa cagagacgcg
      661 cagccgggta tgcatgagaa gcctccagag taggatcgct gtcggggaaa acgcaacagg
      721 ggttcggtca gattgtgcca tgctttcccg cagattgcaa agcccttgtc tcttattgac
      781 cgggtggggg accggtggta gactttgccg tctttttccg gaaggggaat accatgaaaa
      841 agcgcctcaa gacgtatccc atgcgtgagc gaagcaacct ggtgaagctc gcggattttt
      901 cccgcctgcc cgagcccggg ttccgcctta caccgtttct cgatgggttc ccgcagattc
      961 atgttggaaa gacgtttcgg cgcatggtgc aggcgattgt ggccgcgcgc cgggcggaac
     1021 gaccggtcat tctcggaatg ggggcgcatg tcatcaagtg tgggctgagt ccctggctga
     1081 tcgagttgct gcgtcgccgc gtcgtgacgg cgattgccct gaacggagcc ggttccattc
     1141 atgactttga actcgccttc tgcggcgaga cttcggaaga tgtcgcccgg gagctggcgg
     1201 ctggtcgctt tgggtgtgtc gaagagaccg gagcctggat gggcgaagcc atgcgggcgg
     1261 ctcatcggca gggactcggt ttgggtgaag ggttgtatca agccatgaaa gcctgccccg
     1321 aacggtatcc gcatcgtgag atcagcctgc tgtggcagac gggtgaactc ggggtgccgt
     1381 gcacggtgca tgtggccgtc ggtacggatt tcattcatct gcacgaagcg tgcgatggcg
     1441 cggcgttagg ggcgacgacc tttcgcgatt tcgagatctt ctgcgatcag gtcgaacagc
     1501 tcgaaggcgg tgtctactgg aacctcggct cggccgtcgt gctgcccgaa gtgtttctga
     1561 aagcggtttc tcgtgcccat aatctgggac atcatctcgc gggtatgacc acgctcaaca
     1621 tggatatgct ggttcagtac cggtcgatga cgaacgtggt caagcgccca agcctcggag
     1681 tgggtgatgg atttcacctc accggtcatc atgaactcat ggtaccgctg ctggcgcagg
     1741 cggtactgga aggactcgac gcatgctgaa acgaacccgt ctgcaggaaa ttctccagtc
     1801 gtttacccgc ctcgaattgg cggtgctggg cgatgtgatg ctcgacgagt ttctgtgggg
     1861 caaggtcgat cgtatttccc ccgaagctcc ggtgcccatc gttcaggtga aaaacgaaac
     1921 ctcgcgtccg ggcggcgcgg ccaacgtcgc ggccaacatc gtgtctcttg gagctcgagc
     1981 ccacatcgtc ggtgtcgttg gtgatgatgg tgcggggcgc aagctgcgcg aatcgctcga
     2041 aagcgagaaa atcgcgaccg acggtctggt catcgatccg gagcgtcgaa cgacgctcaa
     2101 aacccgtatc atcgggcaca aacagcagat gattcgtatc gaccgcgaaa gcagcggcgg
     2161 catcggtgag gcgtgcggtg aactcatcca cgcggcgcaa cgcggtgcgg gcggcatcca
     2221 gcgaggcgaa agtgaaattg agcgaggcgc gataatgggt ggccagcagc tcgtagcgca
     2281 cttcgcggcc ggtgta
//
LOCUS       DAORHD010000842         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57658_length_2296_cov_3.354306, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000842 DAORHD010000000
VERSION     DAORHD010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57658_length_2296_cov_3.354306"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="PKO06_16140"
     CDS             <1..994
                     /locus_tag="PKO06_16140"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNV71235.1"
                     /translation="VKKTWKQVEVREAGHDYFGLVAAVIASGSNLMAVADYRRDGAAG
                     AVSRDPDAGRRWSIECLTWKRKPLDTQGVAEIAGSPRQKAFGWRDPYGEEVNVISSGA
                     TNLARLRSRQPVPAFRIQQRVTIDPTQPGVATGTWSSPDRWREPPMLGRRERQMLDRL
                     PQKLTHRQVIEWLMIEIGHGIPYRRLPGRMTGERLLELGYGDCSGKARLLCDLARHRG
                     VPARLVGGLLLKPGLIDKTHVWCEVWLEDQWVPVCPTNHLFGRLPAHWLVLRYGETPT
                     LEKPGSLIFRVRELGRKTSSETGSRGKRSDSAQSRHVRDHLPESSASEVMGGSL"
     gene            991..1809
                     /locus_tag="PKO06_16145"
     CDS             991..1809
                     /locus_tag="PKO06_16145"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV71236.1"
                     /translation="MTYNEQMRGRCRQHALRMVMAAVLLAGLGVIVAEAVEVKVIRVN
                     YQRPEDLCETLRRLHGSAARFAEAPNINAIIVSSDQPEIIRAVETLVRELDRRPATLR
                     YEIQSVSAGADAGTQVTLTRKGTFRPETRTSWTQGNQTRVVVGLEGQEIGLIDQTIRV
                     ESIPSGYGYPNQPVVVREDRGMRVRGRLNGNGEVTVEVFNAEGRPDDIRSVSTQMLVP
                     LGQWFEIGGAGDQRSGGGNNVGLSDGRLDGQVQRGRRSDSQTFKLRITVSPESR"
     gene            complement(1896..>2296)
                     /locus_tag="PKO06_16150"
     CDS             complement(1896..>2296)
                     /locus_tag="PKO06_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71237.1"
                     /translation="NSPNELPPYQDNPPPPDDSSLTPPPPPPEGDEPPPPPPDDGDVP
                     PPPPPDDNGDNPPPPPPDGGDGTEPPPPPPDGGDGTEPPPPPPDGGDGTEPPPPPPDG
                     GDEPPPPPPDDGDSPSYRPPEDGGGNEVSF"
BASE COUNT          453 a          600 c          791 g          452 t
ORIGIN      
        1 ggtaaagaaa acctggaaac aggttgaggt gcgtgaagcc ggacacgatt atttcggtct
       61 cgtcgcagcg gtcatcgctt cgggttcgaa cctgatggcc gttgccgatt accgtcgaga
      121 tggagcggcc ggagccgtct cgcgagatcc cgatgccgga cgtcggtggt ccatagaatg
      181 tctgacctgg aaacgaaagc ctctcgatac tcagggtgtt gccgaaatcg ctggttcccc
      241 tcgacagaag gccttcggtt ggcgtgatcc gtacggagag gaagtgaatg ttatatcctc
      301 aggagcgaca aacctggctc gtctgcgttc tcgacaacca gtacccgcgt ttcgtattca
      361 gcaacgggtg acgatcgacc cgacgcagcc gggcgtggca acgggaacct ggagttcccc
      421 tgaccgatgg cgcgaaccgc cgatgcttgg caggcgtgaa cgacagatgc ttgatcgtct
      481 gccacagaag ctgacccatc gacaggtgat cgagtggttg atgattgaaa tcgggcatgg
      541 cattccgtat cgacggttgc ccgggcgcat gaccggcgaa cgtttgctgg aacttggcta
      601 cggcgattgc agcgggaagg cacgcctgct ctgcgatctg gcgcgacatc ggggagttcc
      661 cgcgagactg gtcggcggcc tgttgctcaa gcctgggttg atcgacaaga cccatgtctg
      721 gtgtgaagtg tggctcgagg atcaatgggt gcccgtgtgt ccgacgaatc acctgttcgg
      781 ccgactgcca gcccactggt tggttttgcg atatggagaa acgccgaccc ttgagaagcc
      841 tggcagcctg atctttcgtg tgcgtgaact gggacggaag acgagtagcg agaccggcag
      901 tcgcgggaag aggagtgact cagcgcagag ccggcacgtt cgtgaccatc ttcccgagtc
      961 gtcagcaagc gaggtcatgg gaggaagttt atgacgtaca atgaacagat gcggggaagg
     1021 tgtcggcagc acgcgctgag aatggtgatg gcggctgtgc ttcttgcagg cttgggcgtg
     1081 atcgtggcgg aagcggtcga agtgaaagtc attcgggtga actaccagag acccgaggat
     1141 ctctgcgaaa ccctgcgtcg attgcatggg agcgcggcac gattcgccga agcgccaaac
     1201 atcaatgcca tcatcgtttc ctccgatcag cctgagatca ttcgcgctgt cgagacactg
     1261 gtacgagagc tcgaccggcg cccggcgacg ttgcggtatg agattcagtc cgtctctgct
     1321 ggtgccgatg ccggaacgca ggtcacgctg acccgaaaag ggacgtttcg tccggaaacc
     1381 agaaccagct ggacccaggg aaatcagacc cgtgttgtcg ttggcctcga aggacaggag
     1441 ataggattga tcgaccagac gattcgagtc gagagcattc cctcggggta cggttatccc
     1501 aaccagccgg tggtcgtgcg cgaggatcgc gggatgcggg tgcgcgggcg tctgaacggc
     1561 aacggagaag tcaccgtcga agtgttcaat gcggaaggcc gtccggatga cattcgcagc
     1621 gtctcgacgc agatgctcgt gccgcttgga cagtggttcg aaatcggtgg ggccggagat
     1681 cagcgcagtg gcggtggcaa caacgttggt ctctcggacg gacgactcga cggacaggtg
     1741 cagcgtggtc gcagaagcga ctcacagacg tttaagctgc gtatcaccgt ctcgcctgag
     1801 tccaggtaaa acaaccagat cagattcaga acagaatacg taaagaaaag aaaacacccc
     1861 gaatcagttg ctgattcggg gtgtcgtttc tgctgtcaga acgatacttc gttgccgccg
     1921 ccatcttcgg gtggtcggta gcttggactg tcaccgtcat ccgggggcgg cggagggggt
     1981 tcatcgcctc catcgggcgg cggaggcgga ggttcagtgc cgtctccacc gtcgggcggc
     2041 ggcggtggcg gctcggttcc gtctccacca tcgggcggtg gcggcggagg ttcggtgcca
     2101 tcgccaccat cgggtggggg cggtggcgga ttgtcgccgt tgtcatccgg cggagggggc
     2161 ggtggaacat cgccgtcatc gggcggcggc ggtggtggtt catctccctc gggcggtggg
     2221 ggaggcgggg tgagagagct gtcatcaggt ggtggcggat tgtcctgata cggtggcagc
     2281 tcattcggtg aattcg
//
LOCUS       DAORHD010000843         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57791_length_2293_cov_3.009830, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000843 DAORHD010000000
VERSION     DAORHD010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57791_length_2293_cov_3.009830"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKO06_16155"
     CDS             <1..504
                     /locus_tag="PKO06_16155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71238.1"
                     /translation="SRLDQHRSLPLEKSSFDLVDFTRHTVADSQFEAELGQKSIQAHL
                     PAEPIPVLASREFLRRALENVLRNAVRYTPPQSVVRIELQRSSQTEVVLKVADQGPGA
                     APENLPRLFDPFFRCQEDRARDSGGIGLGLAIVRKAVEAHDGSVTARNLDPNGLEIEI
                     RLPTGNP"
     gene            591..1412
                     /locus_tag="PKO06_16160"
     CDS             591..1412
                     /locus_tag="PKO06_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71239.1"
                     /translation="MKPTIRVLFVACALLIGCLSLATVSAFNRRDLVLVNILWPVVKA
                     GLAAHQNERPLLPAMLQAAAGGAMMKHGLELASECHSNQPGEVWKAKLLFNAGASLAE
                     CAGERNLQFRMDLGPVWMIADQSCIRFRPAVHAMIAPLLNMSDGAKFDLGKSLQFGTT
                     TFRRTPRRDGTITSGGALAYSNANNIITNPSGSHLGHELVHTFQYRRDAFLSVPITHF
                     SPRLQKLLPDNIIDDTGWGINWAIQRCWADWTGKDPDFEIYMEKEAYYLADGVHF"
     gene            1543..>2293
                     /locus_tag="PKO06_16165"
     CDS             1543..>2293
                     /locus_tag="PKO06_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71240.1"
                     /translation="MVFTLIRHKTRILWNQFTAWKGKIAGPMLLLLYLGCSLVIGGGI
                     AELMTQAKSWFPVFFLPWIACFLLIYGLITVFLSDLIIGHTLNWGQMSTDHRLLITLP
                     ISYPTLLLLKLYERLLTDITGVMLLCGSFIALATLSGITCASVLLGLLLFVQVELVIG
                     LLVNLASVVIQRLLPPSGVANTFSLLGYLSAVVVLLPYIWFSNNLPLALSTLYDLYTN
                     WGTWLEWILAPFLLVSGLLTTPFDWWQFLKWE"
BASE COUNT          456 a          681 c          602 g          554 t
ORIGIN      
        1 tcacgcctgg accagcatcg ctcgctgccc ctcgaaaaat cgtcgttcga tctcgtcgat
       61 ttcacccggc acaccgtagc tgacagtcag tttgaagcag aactggggca aaagtcgatt
      121 caggcacatc tgcccgcaga accaattccc gtgctcgcgt cgcgtgagtt cctgagacga
      181 gctctggaaa acgttctgcg aaacgcggtc cggtacactc cgcctcagag cgtcgttcgt
      241 atcgaactgc agcgttcatc gcaaacggaa gtggtgctga aagtggcaga ccagggaccg
      301 ggcgcagcgc cagagaacct cccgagactt ttcgatccct tctttcgttg ccaggaagac
      361 cgggcccgtg attccggggg catcggactg ggtctggcaa tcgtccgcaa agcggttgaa
      421 gcacacgatg gttcggtcac ggcccgtaat cttgatccga atggcctgga gatagaaatt
      481 cgcttgccca ccggcaaccc ctgatctcag gtgatcgtgg caacttctct tcacgaaaag
      541 tgaagttgtg tcgaaagaag ggcagactcg atctgacgag gatgttcttc gtgaaaccaa
      601 ccattcgggt tttgttcgtc gcctgcgcgc tcttgatcgg ctgcctgagt ctcgcaacgg
      661 tgagtgcttt caatcgtcgc gatctggttc tggtcaacat tctttggcct gtcgtcaagg
      721 ctggtctggc ggctcatcag aacgagcgcc ctcttctgcc ggcaatgctt caggcagcgg
      781 caggtggcgc catgatgaag cacggtctcg aactcgcctc agaatgccac agcaatcagc
      841 ccggcgaggt ctggaaagcc aaactgctct tcaacgcggg tgcttcactc gccgagtgtg
      901 ccggtgaaag aaatctgcaa tttcgcatgg atctcggacc ggtctggatg atcgccgatc
      961 aatcctgcat tcgttttcgg cctgctgtgc atgcgatgat cgctcctctg ctcaacatgt
     1021 cagacggcgc gaagttcgat ctcggtaagt ccctgcagtt cggaaccacg acctttcggc
     1081 gcacaccccg ccgtgacggg acgatcacca gtggcggagc attggcctac agtaatgcca
     1141 ataacatcat caccaatccc agcggctcac atctcggaca cgaactggta cacaccttcc
     1201 agtatcgccg agacgcgttc ttgagcgtac caatcactca cttctctcct cggctccaga
     1261 agttgcttcc cgacaatatc attgacgaca cgggatgggg aatcaactgg gcgattcaac
     1321 gatgttgggc tgactggacc ggcaaggatc cagatttcga gatctacatg gaaaaagaag
     1381 cttactatct tgccgacggg gtgcattttt aagatacgcc cccgccgcat ctgaccgtcc
     1441 cggctttttc gaacctctcc cgcacgccgt gtgttacact gccatggcca cgcccatgtc
     1501 catggcggac agtcagggtt ttctcaagga tcatcgtacg agatggtatt cacgctgata
     1561 cgccataaaa cgcggattct ctggaaccag tttacagcct ggaaagggaa gatcgcgggt
     1621 ccgatgctgc ttctgttgta tctgggatgt tcgctggtca tcggtggcgg gattgccgag
     1681 ctgatgaccc aggcgaaaag ctggtttccc gtcttctttc ttccctggat cgcctgcttt
     1741 ctcctgatct atggcctgat caccgtgttt ctctccgatc tcatcatcgg ccatacgctg
     1801 aactggggtc agatgagcac cgaccaccgg ctcctgatca cccttcccat ctcgtatccg
     1861 acgttgttgt tgctcaagct ctacgaacgt ctgttaacgg acatcaccgg agtcatgctc
     1921 ctgtgcggca gcttcatcgc cctggccacc ttgagcggta tcacgtgtgc cagcgtcctg
     1981 cttggtttgt tattgtttgt ccaggtcgag ctcgtcatcg gcctgctcgt caacctcgca
     2041 tccgtcgtca ttcagcgact cctgccacca tccggcgtgg cgaatacgtt ttcgctgctc
     2101 ggatatctca gtgccgtggt ggttctgttg ccctacatct ggttctcgaa caatcttccg
     2161 ctggccctca gcactctcta cgacttgtac acaaactggg gaacctggct cgaatggatc
     2221 ctggcaccct ttcttctggt ttccggttta ctgaccaccc cctttgactg gtggcaattt
     2281 ctcaaatggg aag
//
LOCUS       DAORHD010000844         2289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_57959_length_2289_cov_3.979409, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000844 DAORHD010000000
VERSION     DAORHD010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57959_length_2289_cov_3.979409"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="PKO06_16170"
     CDS             complement(<1..1195)
                     /locus_tag="PKO06_16170"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71241.1"
                     /translation="MSEDSLTTILDTSQPAYSLANELVRLCPELSTREHTGAVALLMA
                     VIAAEHEGHTYLSLNSDDGSGSLSHTVPLILTSDAPREAMSEITRQAADLASSQKLRP
                     LIGSAGEHRPLILEGDTLATHRSSLQEAQLNHLLRQRLYLPDFDVCLPASSEMFPPSQ
                     PGLTTEQAAAVTRALQRRLTVISGGPGTGKTTIVAALIASLCRLGVSPRDIGLAAPTG
                     KAANRLEESISRSLTHLETVTTEAERTALSGVTLHRLLGVGSRQGRPAFHAGNPLPLR
                     AVIVDEASMIDLSLMHQLLEALLPDCRLVLLGDADQLPAVENGAVFRDLVSPQDSQMT
                     TPNWCCRLTQSFRMREEDPDGSAILRLATRIRLSDAGSGTESSSPDRLLPVRPHCSAL
                     NGRGPE"
     gene            complement(1188..>2289)
                     /locus_tag="PKO06_16175"
     CDS             complement(1188..>2289)
                     /locus_tag="PKO06_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNV71242.1"
                     /translation="PDDSCVTAPTDATARTGAETGSSRRTATSIPIQVPTLPIPTDIS
                     RSLARTHAGFRLHSYSSLKQAHSSFELDEQLQWADRRAPDISEVVPPVTPIGHPKPQD
                     ESVSDGTNERATTPSTAAPDELTPQPLTSASLPRGATFGVCVHEVLEQIPLASFASAT
                     FTEWAQHPRVREILDQQQKVLQLDEALIPLFHRFIHAAFTTSLPLPETGTSVRLCDCP
                     EQVREFDFHFCRTTSASVQGDNAVNPDMIKGFIDVLFMVQGRWYFLDWKTDLLPSYVP
                     EALKAVIDDVYSLQAQLYSLALLRLLGLNPTNPDHQEHYDKLMGGYVYVFLRGLDLPG
                     QGVFSGRASWNELTGLHARLLSLSDFEDPAHE"
BASE COUNT          472 a          613 c          742 g          462 t
ORIGIN      
        1 tctccggccc tcgaccgttg agcgcagagc aatgcggacg cacgggaagc aggcgatcgg
       61 gcgaggaact ctcggtgccg gaccccgcgt cactcaggcg gatgcgggtt gccagccgca
      121 gaatggctga tccatccggg tcttcttcac gcatgcggaa actctgggtg agacgacaac
      181 accagttcgg cgttgtcatc tgagagtctt gaggggaaac gagatcgcga aagaccgcgc
      241 cgttttcgac ggcgggcaac tgatcggcat cgcccagaag taccagtcga caatccggaa
      301 gcagagcttc cagaagctga tgcatcaaag acaaatcgat catcgaagct tcgtcgacga
      361 taacggctcg cagtggcagg ggattgcccg cgtgaaacgc agggcgtccc tgtcgactcc
      421 ccacgcccag caatcgatgc agggtgactc cgctcaaggc agtgcgttcc gcttccgttg
      481 tgacggtttc gaggtgggtg agacttcgcg aaatcgattc ttccagacgg ttggcagctt
      541 tgccggtcgg ggcggccagg ccgatatctc gcggggacac cccgaggcga cagaggctgg
      601 cgatcaaggc agcgacaatc gtggttttcc cggtgccggg tcctcccgag atgacggtca
      661 gacgacgttg taaagcacgc gtgacggcag ccgcctgctc ggtggtgagt ccgggttgtg
      721 atggcgggaa catctctgaa gatgccggca agcacacatc gaaatcgggc agatacagtc
      781 gctgccggag cagatggttc agttgtgctt cctgcaggga actgcgatgg gtcgccagtg
      841 tgtcaccttc gagtatcaac gggcgatgtt cacctgcgct cccgatcaag gggcgtagct
      901 tctgactcga agccaggtcg gcggcctgtc gggtgatttc gctcatcgcc tcgcgcgggg
      961 catcagaggt cagaatcagc ggcacggtgt gagacaggct tccagacccg tcatctgaat
     1021 tcagcgaaag ataggtgtgc ccttcgtgct cggcggcaat tacagccatc agaagggcga
     1081 cagcacccgt gtgctcacgt gtcgacagtt cggggcacag acgtaccagt tcgttggcca
     1141 gtgaatacgc tggttgtgag gtgtcgagaa ttgttgtaag agaatcttca ctcatgcgcg
     1201 ggatcctcga agtcgctcag cgaaagcagt cgtgcatgca aaccggtcag ctcgttccac
     1261 gaggcgcggc ccgagaagac tccctggccg ggcaggtcga gcccgcgtag aaagacatag
     1321 acgtagccac ccatcagttt gtcatagtgt tcctggtggt ccggattcgt cgggttcagg
     1381 ccgagcaggc gcagcaatgc cagcgaatac agctgagcct gcagagaata cacatcatcg
     1441 attaccgctt tcagtgcttc tggaacatac gatggcagca gatcggtttt ccagtcgaga
     1501 aagtaccatc gcccctgaac catgaaaaga acgtcaatga atcctttgat catgtcggga
     1561 ttcacagcgt tgtctccttg tactgatgcg cttgtcgtgc gacagaaatg aaagtcgaat
     1621 tctcgaacct gctcgggaca gtcgcacaat ctcacgctgg ttccagtttc cggcagtggc
     1681 agggaagtcg tgaacgcagc atggatgaat cgatggaaca gaggaatcag tgcctcgtcg
     1741 agctggagta ctttctgctg ctggtcgaga atttcccgca cgcgggggtg ttgggcccat
     1801 tccgtgaagg ttgcggatgc aaacgatgcg agcggtatct gctcgagcac ttcgtgaacg
     1861 cagactccga acgtggctcc gcgcggcaga gacgctgacg taagcggttg cggcgtcagt
     1921 tcgtcaggtg ctgcagtcga tggggtggtc gcgcgttcgt tggttccgtc ggaaacactc
     1981 tcgtcctgtg gcttcgggtg accgatggga gtcacggggg ggacaacctc ggaaatgtcg
     2041 ggagcgcgcc ggtcagccca ctgaagctgc tcgtcgagct cgaacgaact gtgagcctgt
     2101 ttcagcgatg agtaggaatg cagtcgaaaa ccggcatgtg tccgagccag tgaacgtgaa
     2161 atgtcagtcg gaatgggtag tgtcggaacc tgaatcggaa tggaggtggc ggttcttcgc
     2221 gagctcccgg tttccgctcc tgttctggcg gtggcgtccg tgggtgccgt gacgcaggag
     2281 tcgtcgggc
//
LOCUS       DAORHD010000845         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58292_length_2281_cov_3.689128, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000845 DAORHD010000000
VERSION     DAORHD010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58292_length_2281_cov_3.689128"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2281
                     /locus_tag="PKO06_16180"
     CDS             <1..>2281
                     /locus_tag="PKO06_16180"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV71243.1"
                     /translation="GCPPDEIEKIGRYLIEEQKLHTTIKLNPTLLGPRELRHILNEEL
                     GWGTEVPDLAFEHDLKYPDALKLIKNLRDCAKKAGVHFGLKLTNTLESINNKKVFPPN
                     EKMMYMSGRALHPISVKVGQKLQNEFKGELDLSFSAGADCYNISDLLACNIKPVTVCS
                     DILKPGGYMRLTQYLEEARQHCAKLKVKDIESLVKKTAGGKKKPIEAALANLNHYADQ
                     VTGNPAYRKDSRLGEHIKTGRPLTTYDCVQAPCIGTCPANQDIPKYLYQVAQGNDAAA
                     MEVILRTNPLPNVAGLVCDHQCQTKCTRLNYDQPLLIREIKRFVAERQKQQGLPQPAA
                     PNGLRVAIIGAGPSGLACAYFLVQDGFQVDVFETKTFAGGMISDAIPTFRLTAEAIAK
                     DVDHIASLGVKFHYQHHIDKQRFSELQRSYDFVYLAIGAQKAKRLGIPNDQAKGVVDF
                     LWFLSQVRRGHKVDLGKNVIVLGGGNSAMDVARTAHRCVGKGGEVQIVYRRTRNEMPA
                     DREEIEALLHEGIKIRELTAPVSVVLENGKAVALRCSRMQLGEPDADGRRKPVKIEGA
                     DFDIAADSILPAIGQDVALDFVDGDLGTDPITGQTKLAKVFAGGDLVRGAATVIKAIG
                     DGKGVALNIIKAANRRIPLFAKRESKGLSRLEYRLRRAKRLPGAQIPTIEGQDRKTFK
                     LVNLRLDEATARAEAARCLFCDDFCGICATVCPNRANVNYTAQPGDYPVFRGVRDGDV
                     LVLKQEGTFRVEQEPQVLNV"
BASE COUNT          507 a          731 c          648 g          395 t
ORIGIN      
        1 ggctgtccgc ccgacgagat cgagaagatc ggtcgctatc tcatcgagga acagaagctg
       61 cacaccacca tcaagctcaa cccgaccctg ctcgggccgc gcgagctgcg ccacattctg
      121 aacgaagagc tcggctgggg tacggaagta cccgatctcg ccttcgagca cgatctcaaa
      181 tatcccgacg cgctcaagct cattaaaaac ctgcgcgact gcgcgaagaa ggctggcgtg
      241 cacttcggtc tcaaactgac caatacgctt gaaagcataa acaacaagaa ggtgtttccg
      301 cccaacgaga agatgatgta catgagcggt cgcgctctac acccgattag cgtcaaagtc
      361 gggcagaagc tgcagaacga attcaaaggg gaactcgacc tgtcgttctc tgccggcgct
      421 gactgctaca acatcagcga tctgctggcc tgtaacatca agccggtcac cgtctgttcc
      481 gacattctca aaccgggcgg ctacatgcgt ctgactcagt atctcgaaga ggcccgccaa
      541 cattgcgcca agctcaaggt caaagacatc gagagcctgg tgaaaaagac cgccggcggc
      601 aagaagaagc cgatcgaagc cgcgcttgcg aacctcaatc actatgccga tcaagtcacc
      661 gggaacccgg cgtatcggaa agacagtcgt ctcggtgaac acatcaagac cggccgcccg
      721 ctgaccacgt acgactgcgt gcaagcgccc tgcatcggca cctgccccgc gaatcaggat
      781 attcccaagt atctgtacca ggtcgcccag ggcaatgacg cagccgccat ggaagtgatt
      841 ctgcgcacca acccactgcc caacgtcgcc gggctcgtct gcgaccacca gtgccagacc
      901 aagtgtaccc gtctgaatta cgaccagccg ctgttgatca gagagatcaa gcgtttcgtc
      961 gctgagcgcc agaagcagca gggcctgccg caaccggcgg caccgaacgg cctcagggtc
     1021 gccatcatcg gtgcaggccc gtctgggctc gcgtgcgcgt actttctggt gcaagacggc
     1081 tttcaagtcg acgtgttcga aaccaaaacc ttcgccggcg gcatgatctc cgacgccatt
     1141 ccgaccttcc gactcacggc cgaagcaatc gccaaagatg tcgaccacat tgcctcgctt
     1201 ggagtgaagt ttcactacca gcatcacatc gataaacagc gtttcagcga actgcagcgc
     1261 tcgtacgatt tcgtctatct cgccatcggg gcccagaaag ccaagcggct cggcattccc
     1321 aacgatcagg ccaagggggt cgtcgacttc ctgtggttcc tgtcacaggt gcgccgcggg
     1381 cacaaggtcg atctcgggaa aaacgtcatc gtgctgggcg gcggtaactc cgccatggat
     1441 gtcgcccgca ccgcgcaccg ttgcgtcggc aaaggcggcg aagttcagat cgtctatcgc
     1501 cgtacccgta acgagatgcc cgccgaccga gaagagatcg aagcgctgtt gcatgaaggc
     1561 atcaagattc gcgaactcac cgcgccggtc tcggtcgtgc tcgaaaacgg caaggccgtc
     1621 gctctgcgct gctcacgcat gcagctcggc gaacccgacg ccgacggccg ccgcaagccc
     1681 gtcaagatcg agggcgccga tttcgacatt gccgccgact cgattctgcc ggccatcggg
     1741 caggatgtcg cgctcgattt cgtcgacggc gatctgggta ccgacccgat caccggccag
     1801 acgaagctgg cgaaggtctt cgccggcggt gatctggtgc gcggggccgc gacggtcatc
     1861 aaggccatcg gtgacggcaa gggcgtggcc ctgaacatca tcaaggccgc caaccgccgc
     1921 attccgctgt tcgcgaaacg cgaaagcaaa ggcctcagtc gcctcgagta ccggttgcgt
     1981 cgcgcgaaac gcttacccgg agcgcagatt ccgactattg aaggacaaga tcgcaaaacc
     2041 ttcaaactgg tcaatctgcg gctcgatgaa gcgaccgccc gcgccgaggc cgcgcgctgc
     2101 ctcttctgcg atgacttctg cggcatctgt gccacggtct gccccaatcg cgccaacgtg
     2161 aactataccg cgcagcctgg cgattatccg gtgtttcgcg gcgtgcgcga cggcgatgtg
     2221 ctggttttga aacaggaagg aaccttccgc gtcgaacagg aaccgcaggt gctcaacgtc
     2281 g
//
LOCUS       DAORHD010000846         2279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58352_length_2279_cov_3.232914, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000846 DAORHD010000000
VERSION     DAORHD010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58352_length_2279_cov_3.232914"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2279
                     /locus_tag="PKO06_16185"
     CDS             <1..>2279
                     /locus_tag="PKO06_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71244.1"
                     /translation="PEVYEALVGDRGAFYVDARAETLMGPELCTTLGIRDLTSVTLVD
                     VARNLLEHAESLRPISRIVYEWIERGLREETIDPVALLGIVAGKPFLLTDDGRYWAPH
                     QTVGFRGFAWFGNRRGYWESGVRQFPLLCRACQIPTEPDVATLEQFLKELHQSVVTYG
                     DASILQNDTGLVHLLMHNYVTIGLYAKRLNREWKVCLCREVGPGVCQDPPLMRLVTLD
                     EPALYLSDTPSLEQEFARAGRFCVVLRASGSEQEKVDAFYLTQGIQRIRDAYRVVVER
                     DQLADRTNEYQRQILSIRALIRSLAAIVPRIRLGRPSLLAGGWQETKLHLLGSSGAVR
                     AIAQLSVKMILPGVGEVMSSAPAVYDADEGRLLLDTQVLERPEDFCGELASGLLPCLY
                     QGPGEEGLRDLLEILLPLRTEEAMYAYLDRRHFPTAARLQGPLERLGERLGQILDFGW
                     PTRFERRFPEIGAPAWERWRHAGFLRHLSQVASLPEHADEYAGWVTALLPELMQEIEL
                     RSDQEEVRRLLLETLKAESLSDVPLELFAEVKTTASTSVSRPTSTEALEQILPGSSGL
                     LSGGTSGNEAHQGQTNLPVIDPASWQPAPVASSEQGLFGRVMSWLFGASKVVASESRF
                     APRLSISMPEPATSAEACVREALIVQDWVSWNPTPVPAPWQFGIHTLAGVFSSESQSW
                     SCEDYELVLATGTEEQRAVRGQVRLQVRVGGGEHLLPMPLYSRLHSGPTVIHGEGALR
                     VAGVMETGGILVFLQTAKH"
BASE COUNT          398 a          617 c          759 g          505 t
ORIGIN      
        1 tccggaggtc tacgaagcgc tggtcggtga ccgtggcgcc ttctatgtcg acgctcgcgc
       61 cgaaaccctg atggggcctg agttgtgtac aacccttggc atacgcgatc tgacctcggt
      121 gactctggtc gatgtcgccc gcaatcttct tgagcacgcg gagtctctgc gcccgatctc
      181 acggattgtc tatgagtgga tcgaacgtgg gttgcgtgaa gaaacgattg acccggtcgc
      241 tttgcttggc atcgtggctg gcaagccgtt tctgctgact gatgatgggc gttactgggc
      301 gccgcaccag accgtcggtt ttcgcggatt tgcgtggttc gggaatcgtc gcgggtactg
      361 ggaatcgggg gtgcgtcagt ttcccttgct ctgtcgggca tgccagattc cgaccgaacc
      421 ggatgtcgcg actctcgaac agtttctgaa agagttgcac cagtcggtcg tgacctacgg
      481 tgacgcatcg atcttgcaga atgatactgg tctcgtgcat ctgctcatgc ataactacgt
      541 gactattggc ttgtacgcaa aacgcttgaa ccgtgagtgg aaggtgtgcc tgtgccgcga
      601 agtcggaccg ggggtctgcc aggacccgcc cctcatgaga ctggtgacgc ttgatgagcc
      661 ggcgttgtat ctgagtgaca caccgtcgct cgaacaggag tttgcccgtg ccggtcgatt
      721 ttgcgtcgtt ctacgcgctt cgggttcgga gcaggaaaaa gtcgatgcgt tctatctgac
      781 tcagggcatt cagagaattc gcgatgcgta ccgcgtggtg gtcgagcgtg atcagctggc
      841 ggacagaacg aatgagtatc agcggcagat tctctcgata cgagctctga tccgcagtct
      901 ggcggctatt gtgcctcgga ttcgactcgg acgccccagt ctgcttgccg gaggatggca
      961 ggagacgaag ctgcatctgc tggggagttc cggagccgtg cgcgcgatcg cgcagctgtc
     1021 ggtcaaaatg attctgcccg gtgttggaga agtcatgagc agtgctccgg cggtctatga
     1081 cgccgatgag gggaggctat tgctggatac gcaggtgctc gagcgtcccg aggacttctg
     1141 tggtgagctg gcttcagggc tgctgccctg tctgtatcag ggacctggcg aagagggtct
     1201 gcgcgatttg ctggagattc tgctgccgtt gcgcaccgaa gaggcaatgt atgcgtatct
     1261 cgaccgtcgc catttcccga ccgccgcacg cctgcagggc ccactcgagc gtctgggaga
     1321 gcgtctgggc cagattctcg attttggctg gccgacccgc ttcgaacgcc gattccccga
     1381 gatcggagcg ccggcctggg aacgctggcg gcatgcggga tttctgcgac atctctcgca
     1441 ggtggcttct ctgccggaac acgcggatga gtacgcgggc tgggttaccg ccctgctgcc
     1501 cgaactgatg caggagatcg agttgcggag tgaccaggaa gaggtgcgtc gccttctcct
     1561 ggaaactctc aaagcggagt ctctcagcga tgttccgctc gaactttttg ccgaagtcaa
     1621 gaccaccgcc tcgacttccg tctcgaggcc gacatcgacg gaagctctgg aacagattct
     1681 gcccggatcg agcggattgt tgtctggcgg aaccagtggg aacgaggctc accaggggca
     1741 gacgaatctg ccggttatcg accctgcgtc gtggcaaccg gctccggtgg cgagttccga
     1801 acagggattg ttcggccgcg tgatgagctg gctgttcggg gcctcgaaag tggtcgcttc
     1861 ggaaagccgg tttgcgccca gactgagcat ttcgatgccg gaaccggcga cgtctgcgga
     1921 ggcctgtgtt cgtgaagcgc tcatcgtgca ggactgggtg agctggaatc cgacacccgt
     1981 gcctgcgcca tggcagttcg gcattcatac actggccggg gttttttctt cggaatcaca
     2041 gagctggagc tgcgaggatt acgagttggt gctggcgact ggcaccgagg aacaacgagc
     2101 ggttcgcggt caggtgagac tgcaagtccg ggttggcggc ggcgaacatc tgcttccgat
     2161 gccgctttac tcgcgtctgc actcgggtcc gacggtaatc cacggagaag gggctttgcg
     2221 ggttgcgggc gtgatggaga ccggagggat tctcgtgttt ctgcagacgg ccaagcacg
//
LOCUS       DAORHD010000847         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58378_length_2278_cov_3.857850, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000847 DAORHD010000000
VERSION     DAORHD010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58378_length_2278_cov_3.857850"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            125..1072
                     /locus_tag="PKO06_16190"
     CDS             125..1072
                     /locus_tag="PKO06_16190"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV71245.1"
                     /translation="MYWSTIRCAVLILLVLLPNWVQGQHSSTASAPVAVENLVRMGFH
                     WNVFSGVERRDAAISLNSWASIIARDMGGKFQTTTEIIDDAQDLFNRLRDNSLDVAIV
                     SAWEYLQYPDHSAFEPVISSIELEHPSQPHVLLVHKDSGITTLRHLQGKSIVINDYGV
                     SDTALRWLDILLAREGLAEPEEFFSSITRSQTISKVVHPVFFRQVDASIANDTSLATL
                     IEMNPQIQKNLKILATSPLIPLGIVFLRKDMEAEKRRTFLDSCLKLDTHAKGQQILTL
                     FKIKRLARFVPETLEPVIAIHREWQQLRKSGSKAPHDSR"
     gene            complement(1069..2106)
                     /locus_tag="PKO06_16195"
     CDS             complement(1069..2106)
                     /locus_tag="PKO06_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71246.1"
                     /translation="MTIDLIVFLVVVGFLVFIGLPTIRNTITLPSEMAMEKVSKGQLS
                     QKLTAHFAKIDSELSEIGFKALETFRVTNLGSDNMLRLYLHADRQTFALATCLAQNTP
                     DGRIGENYLEFIDEFEDGRSLTTRNSEITSVFASDPRKPLIVAANAVPAELYRLHREA
                     RQQYTVAGRSWSHADELLKRGSESHREMCEFQVSCGRLEFEPATQRFRATWKLALIGL
                     VNFVNPLSDHFTWFRFVGLVIVVGLLPIIGTVGLAALAQHLSLSPSEMPLLLMGCRVV
                     LFGFAGLIVGWTFPGKVFIWSFLATYLPQITLIESNEWTFVYSIWAAFVGERVSSWQL
                     SRRDLLREERN"
BASE COUNT          572 a          633 c          587 g          486 t
ORIGIN      
        1 cacccaccat cttcgcatct gatacgaaag aaacgatcgc gacgcgagaa ccggaaccac
       61 aggctgcagg ttcggttcat gacatgatag aatcatgttt gtgaggtatc acttgagatc
      121 attgatgtat tggtcaacca ttcgctgcgc cgtcctcatt ctgcttgtcc tgctgccgaa
      181 ttgggtacag ggccagcatt cgtcgacggc atccgcccct gttgctgtcg aaaatctcgt
      241 gcgcatgggg ttccactgga acgtcttcag cggagtcgaa cgacgggatg cggctatctc
      301 gttgaattcg tgggcaagca ttattgctcg cgacatgggg ggaaaattcc agaccacgac
      361 ggaaatcatc gatgacgcgc aggacctttt caatcgcctt cgagacaaca gcctcgacgt
      421 cgcgattgtc tctgcctggg aatatcttca gtatccggat cattccgcct ttgagccggt
      481 catctcctca atcgagttag agcatccgtc tcagccgcat gttctgttgg ttcataaaga
      541 ctctggcatc acgaccctcc gccacctcca aggcaaaagt atcgtcatca acgactacgg
      601 tgtctcggac acggctctcc gctggctcga cattcttctc gcccgcgaag gattggcgga
      661 gcccgaggag ttcttctcgt ccattacccg gtctcaaacg atttccaagg tggtgcatcc
      721 cgtgtttttc cggcaggttg acgcttccat cgcgaacgac acttctctcg ccaccttgat
      781 cgaaatgaac cctcaaattc agaagaacct caagattctc gcaacgagcc ctctcattcc
      841 gctcgggatc gtctttctca gaaaagacat ggaagcagag aaacgtcgaa cctttctcga
      901 ttcgtgtctg aagctggaca ctcacgcgaa aggtcagcag attctgaccc tgttcaaaat
      961 caaacgtctc gcccgtttcg ttcccgagac gcttgaaccg gtgatcgcca ttcatcgcga
     1021 gtggcaacag cttcgcaaaa gtggcagcaa agcgccacac gattcgcgtt agttccgctc
     1081 ttcgcgcaga aggtcacgcc gagacagttg ccaggaagac acccgctcac cgacaaaagc
     1141 ggcccagatc gagtacacaa aggtccattc attcgattcg atgagcgtga tctgcggcag
     1201 atacgtcgcg agaaagctcc agatgaacac ctttcccgga aaggtccagc cgacgatgag
     1261 gccggcgaaa ccaaacagga caactcggca ccccatgagc agcagcggca tttcagatgg
     1321 agacagggac agatgttgcg ccagagcggc gagcccgacc gtaccaatga tcgggagcag
     1381 gccgacgacg atcaccagac ccacgaagcg aaaccaggtg aagtggtcgc tcagcggatt
     1441 gacgaagttt acgagaccga tcaacgccag tttccaggtg gcccgaaatc gttgcgtcgc
     1501 gggttcaaac tccagacgac cgcaggacac ctgaaactca cacatttcac gatgagattc
     1561 gctgcctctc ttcagcaact cgtccgcatg cgaccagctg cgcccggcaa ccgtgtattg
     1621 ctgtcgcgcc tcacgatgaa gccgatataa ctcagccgga acggcattgg ccgcgacaat
     1681 caagggtttc cgcggatcag aagcgaaaac agacgttatc tcggaattgc gtgtggtcag
     1741 actgcggcca tcttcgaact cgtcgatgaa ctcgagatag ttttctccga ttcgtccatc
     1801 cggggtgttc tgtgccaggc aagtggcaag agcgaacgtc tgtctgtcgg catgcagata
     1861 gagccgtaac atgttgtctg atccgagatt cgttacccgg aaagtttcga gagctttgaa
     1921 gcctatctca gacaattcgc tatcgatttt agcaaagtgg gccgtcagtt tctgcgacag
     1981 ttgccccttc gacactttct ccatggccat ttcagacggg agggtgatgg tgttacgaat
     2041 tgttggcaga ccgatgaaga caagaaaacc aaccaccacc agaaagacaa tcaaatcgat
     2101 ggtcatggca actcctgtga ggtgagaaag ctccgcgact cattgtaaca gacaactcag
     2161 cgtgaattga acaaatgaaa cgcaaaagga aactgccgca taccggttta tcagtatgcg
     2221 gcagtaaaac gagctaaaaa ggttgagtga ccaggtcaga gaatgagcga actggaga
//
LOCUS       DAORHD010000848         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58627_length_2272_cov_3.397384, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000848 DAORHD010000000
VERSION     DAORHD010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58627_length_2272_cov_3.397384"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1875)
                     /locus_tag="PKO06_16200"
     CDS             complement(64..1875)
                     /locus_tag="PKO06_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV71247.1"
                     /translation="MPNRMTWWKRLLIGAAAMLVVGVGAGEAVPKASRNVETYFGPHA
                     NHRLTIDNHFISLLDSAKKTIDGAFFELRLDSIADAFLRAHERGVKIRLVTDSDYVGN
                     EWMQKLIEAGIEVVEDDRSALMHNKFAIVDGTTVWTGSYNLTDSCSWRNNNNAMCIRS
                     PELAKVYQREFNEMYEDGQFGVTSPSTIDQQSVTLRIGNQNANIQVFFAPEDKPNLRI
                     AELLKKARKQILFMHFAFTADQISDVLIEKFRSGVDVTGIFDSRLYRSTGPYSEFFKL
                     TNADVPIILADNREGKLHHKVFIVDPGEPDGFVISGSLNSSNNGDGTNDENVIVIHNQ
                     EIAQSFYQEFKKLRGTFSQCSATTVNFFLKPETKVPSFNVVFNANGRPVERIEVMYPA
                     RWKLGGEEKVSIYRLDQRPLPKGSVTFHPKGFTIENASLTPFGKSSYLVFNFKNLVTP
                     KIQGGYNLYVRAGAKVKGKMTPLQNQPGIQVTEKIGTTESAVCFETLLQKMRLAFSEL
                     LKMQGSGMNDKDFEKMFAGWREHYDEITEVILNDARNGEYTKLDQFFTLYYQLPVEDR
                     KRLEGFMRNIKTVLKVNAAHGDEAATERVELLKRNEK"
     gene            complement(2011..2208)
                     /locus_tag="PKO06_16205"
     CDS             complement(2011..2208)
                     /locus_tag="PKO06_16205"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71248.1"
                     /translation="MSVEPEECVVFEDAPGGIEAAGRGGMRAVGITTMIDAEVFRRQP
                     HVIHTAQQFTELRPEMFLCTA"
BASE COUNT          500 a          660 c          571 g          541 t
ORIGIN      
        1 tcgggccaaa cacaatcccc cccggacgtt gtccgggggg gattgtgttt gatcgggtcg
       61 ctgttacttc tcgttgcgtt tcagcaactc aacgcgttcc gtggccgcct catcgccatg
      121 agccgcattg actttcaaca cggtcttgat gttgcgcatg aagccttcga ggcgtttgcg
      181 atcttcgacg ggaagttgat agtacaaggt gaaaaactga tcaagcttgg tatattcacc
      241 atttcgagca tcatttaaaa tcacttcggt gatctcatca tagtgttcgc gccagccggc
      301 aaacatcttc tcgaagtcct tgtcgttcat gcccgatccc tgcatcttca gcagttcgct
      361 gaacgccaga cgcattttct gcagcaacgt ttcaaagcag acggccgatt ccgtcgtccc
      421 gattttctcg gtgacctgga ttccaggctg attctgcagc ggggtcatct ttcccttgac
      481 cttggccccg gcacgaacgt acaagttata cccgccttga atcttgggtg tgacaaggtt
      541 tttaaagttg aataccagat agcttgattt gccgaatggc gtcagactgg cattctcgat
      601 ggtaaacccc ttggggtgaa acgtgaccga tcccttcggc agaggccgct ggtcgagacg
      661 atagatactg actttttctt ccccacccag cttccaccgt gccggataca tgacctcgat
      721 ccgctccacc gggcgaccgt tcgcgttgaa cacgacattg aacgagggaa ccttggtttc
      781 cggtttgaga aagaagttta ccgtggtcgc ggagcactgc gagaacgttc cccgcagctt
      841 cttgaactcc tgataaaaac tctgcgcgat ctcctgattg tgaatcacga tcacgttctc
      901 atcgttcgtc ccatcgccat tattcgatga gttgagcgaa cccgaaatca caaatccgtc
      961 cggctcaccg ggatcaacga tgaacacttt gtgatgcagc ttgccctcgc gattgtcagc
     1021 cagaatgatc gggacgtcag cattcgtcag tttgaagaac tccgaatacg ggcccgtcga
     1081 gcggtagagg cgtgaatcga aaattcccgt gacatcgacc ccgcttcgaa acttctcaat
     1141 cagaacatct gagatctgat ccgccgtgaa ggcgaagtgc atgaacagaa tctgcttgcg
     1201 agcctttttc aacaactcag caattcgcag attcggctta tcttcgggag cgaagaacac
     1261 ctgaatgttc gcattctggt tcccgatgcg cagcgtaacc gactgctggt cgatggttga
     1321 gggcgaggtc acgccgaact gcccgtcttc gtacatttcg ttgaactcgc gctggtacac
     1381 tttggccagc tcaggcgagc gaatacacat ggcgttgttg ttgttgcgcc agctgcatga
     1441 atcagtcaga ttataggatc cggtccagac cgtggtacca tcaacgatgg cgaacttgtt
     1501 gtgcatcaat gccgagcgat cgtcttcgac cacttcgatg cccgcttcga tcagtttctg
     1561 catccattca ttgccaacgt agtcactgtc ggtcaccagt cgaatcttga ccccgcgttc
     1621 atgagcccgc aaaaaggcgt cggcaatcga atcgagtcgc agttcgaaaa atgctccgtc
     1681 aatcgtcttc ttcgccgaat cgagcagact gatgaaatga ttgtcgatcg tcaggcgatg
     1741 attggcgtgt ggtccgaaat aggtttcaac attccgactc gctttcggaa ccgcttcccc
     1801 ggctccgact cccacgacca gcattgcggc agctccaatc agaagccgct tccaccacgt
     1861 cattcggttc ggcatgcgcg tccctttccc gacaaaatcg atctaaaata cttcggtacc
     1921 tctcatcata agaatccggt gaggaagcgt caagcaacca actcgcccgt tacaggcctg
     1981 gccagctgga acgggtggcg gcgaaatcgg ctaagccgtg cacagaaaca tctcgggccg
     2041 caactcagtg aactgctgcg ccgtgtgaat cacgtgcggc tgacgacgaa acacctcggc
     2101 atcgatcatg gtcgtaatac ctaccgcccg catgccacca cgaccagcgg cctcgattcc
     2161 acctggcgcg tcctcgaaaa cgacgcactc ctcgggttcg acgctcaggc gtcgggcagc
     2221 cagcagaaaa agatctggcg ctggttttcc ccgttcgacc tcgcccccgt gt
//
LOCUS       DAORHD010000849         2271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58661_length_2271_cov_4.634477, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000849 DAORHD010000000
VERSION     DAORHD010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58661_length_2271_cov_4.634477"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PKO06_16210"
     CDS             complement(<1..568)
                     /locus_tag="PKO06_16210"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71249.1"
                     /translation="MHTRSQQPVRSSQVLLQLCLLLLWVSSAMAQIDPQLLYQQALNE
                     YSMRHLKEARRNYETLIRDYPQHPLARRAAIELARILADLREFDKAIERLTPVADSPD
                     ASYERKMARDALLKMLAELQRFKPAVDLLEKWRASQPEDLSLARQLADFYLQTGREDE
                     ARTLLEGLLERTADHEVFRDLLQLATRSG"
     gene            complement(573..>2271)
                     /locus_tag="PKO06_16215"
     CDS             complement(573..>2271)
                     /locus_tag="PKO06_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71250.1"
                     /translation="EIKLDPKDLEKFEQAFKMEDYMKNLDHTINLLDQLKNEMEMNAL
                     GEALQDLHRRQERLASATKELEDKAKPGEPLSQEQKTRLEQVKAQRQQLEEKQKQSGS
                     LTPDEQAQLDALKAEQQKLEEQAQKDGQLSAEDRAKLEDLKAQQEKIRQELEELQKKA
                     QEMAQRKPPEGEQQKGVQEDLKNIADRMKQEDFQKTSEEIKKNLDDKKFSDARQNQQQ
                     MLKFLESLSKEGKKVCEACSGGGQQQQLDLSRYVKRALQVSFDQETLIKRLDGIPGQF
                     MRGQMPAVEGRVDECSMQELLVKKQAQDLETALDTLVRSSFTIDPDVMNHLKGVQNVF
                     SDIVKDLEDRAIDKSRGQMREIVRRFNLLAMDLMRAQDEQSQNSSSSPQNALQQFKQL
                     MQRQLSLHQQMQKMQQMNPQDAQMMQQLQKMAMEQRMIREALEKLMRESKQQMQTLGR
                     MDDVLKDMEDLETKILDPNLRRDVAKKQKDIYDRMLKATKSLKRRDEESEERKAEKVK
                     RELVQTEPEKPLPQLGSNSVDLSKDFLGELKEEFPRTYEPELRDYYKSLNLYGTENR"
BASE COUNT          361 a          651 c          643 g          616 t
ORIGIN      
        1 ggcccgatcg ggtggcgagc tgcagcagat cgcgaaaaac ctcatgatcg gccgtgcgtt
       61 ccagcagccc ttcgagcagc gttcgcgctt cgtcttcgcg cccggtctgc aggtagaagt
      121 cggcgagctg gcgcgccagc gacagatctt cgggctggga agcacgccac ttttcgagca
      181 ggtcgaccgc cggcttgaag cgctgcagtt cggcgagcat cttgagcagc gcatcacgcg
      241 ccatttttcg ctcataggag gcgtcaggac tgtcggcgac gggggtgaga cgttcgatcg
      301 ccttgtcgaa ttcccgcaga tcggccagaa tgcgcgccag ctcaatggct gcacgtcggg
      361 ccaggggatg ctgcggatag tcacggatca gcgtttcata gtttctgcgc gcctctttca
      421 aatgacgcat gctgtattcg ttcaatgcct gttggtacag cagctgtggg tcgatctggg
      481 ccatggcact cgacacccag agcagcagaa ggcacagctg cagaaggact tgagacgaac
      541 gtaccggttg ctggctgcgt gtatgcatag tatcagcggt tttccgtgcc atagagattg
      601 agcgacttgt agtagtctcg cagttcgggc tcgtaggtgc gtgggaactc ttccttcagt
      661 tcgcccagaa aatccttcga caggtcgacg gagttcgatc ccagttgtgg caacggcttc
      721 tcgggctcgg tctgcaccag ctcgcgcttg accttttccg ccttgcgttc ttcgctctcc
      781 tcgtcgcgtc gcttgagaga cttcgtggcc ttgagcatgc gatcgtaaat gtctttctgc
      841 ttcttcgcca catcgcgtcg cagattcgga tcgagaatct tggtttcgag atcttccatg
      901 tccttgagga cgtcgtccat gcgacccagc gtctgcatct gctgtttact ctcgcgcatc
      961 agcttctcga gagcctcgcg aatcatgcgc tgttccatgg ccatcttctg cagttgctgc
     1021 atcatctgcg catcctgtgg attcatctgc tgcattttct gcatctgctg gtgcagactg
     1081 agttgtcgct gcatcagttg cttgaactgc tgcaaggcgt tctgagggct tgacgatgag
     1141 ttctggctct gttcatcctg ggctcgcatc aggtccatgg ccagcaggtt gaaacggcgt
     1201 acgatctcac gcatctggcc tcgtgatttg tcgatggcgc gatcttccag gtccttgacg
     1261 atgtcgctga acacgttctg cacgcccttc aggtggttca tgacgtcggg atcgatggtg
     1321 aaactcgaac gtacaagagt gtcgagtgcg gtttcaagat cctgagcctg tttcttgacc
     1381 agaagttcct gcatcgagca ttcgtcgacc cggccttcga cagccggcat ctgtccgcgc
     1441 atgaactgtc cgggaatgcc gtcaagtcgc ttgatcagcg tttcctggtc aaacgacacc
     1501 tgcagcgcgc gcttgacata gcgtgacaga tcgagctgct gctgctgacc accgccggaa
     1561 cacgcctcgc agactttctt gccttccttg cttaacgact cgagaaactt cagcatctgt
     1621 tgctgattct ggcgggcatc cgaaaacttc ttgtcgtcga gattcttctt gatctcttca
     1681 gaggtcttct gaaagtcctc ttgcttcatg cggtcggcga tgttcttcag atcctcttga
     1741 acgcccttct gctgctcgcc ttcagggggc ttgcgctggg ccatttcctg ggccttcttc
     1801 tgcagttcct cgagttcctg ccggatcttt tcctgctgtg ccttcaggtc ttcgagtttg
     1861 gcgcggtcct cggcgctcaa ctggccgtct ttctgggcct gctcttcgag tttctgctgc
     1921 tcggctttga gagcatcgag ctgggcctgt tcgtcagggg tcagagaccc tgattgcttc
     1981 tgcttctctt ccaattgttg acgctgagcc ttgacctgct cgagccgcgt tttctgttcc
     2041 tgtgagagtg gctcgcccgg tttggccttg tcttcgagtt ccttggtcgc cgaagccaga
     2101 cgttcctggc gccggtgcag gtcctgcagg gcttcgccga gcgcgttcat ttccatctcg
     2161 ttcttcagct gatcgagcag gttgatggtg tgatcgaggt tcttcatgta gtcttccatc
     2221 ttgaaggcct gctcgaactt ttcgagatct ttcgggtcga gcttgatctc c
//
LOCUS       DAORHD010000850         2269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58772_length_2269_cov_2.692864, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000850 DAORHD010000000
VERSION     DAORHD010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58772_length_2269_cov_2.692864"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKO06_16220"
     CDS             complement(<1..1104)
                     /locus_tag="PKO06_16220"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV71251.1"
                     /translation="MKPRILVISADFPPHAWPTGIRAGKLCRELARDYEVHVITTAAA
                     SNQIAGVTVHRVLPVSWVSTLVQTLPKWRLQHVGELLLWPLCWPDWTNVWALRAFRRA
                     RSLHQRLGFAAVVGFVLPFAGALAGLLLKQVEKVPLIANLDDSPTDPDFFYQYRTWWH
                     YLAFRRFENDLPAAADAVVYVSQANLERVAKRIPEHRHRLHLIRYGADAAAFVSRSTD
                     SGNAPSMVMKYIGNAGGWEYYRSQPYSTVRALLRKLLHPGLFEYAEHRSETQTPCLMG
                     KAIHEVNRRNPETVGRIQLVAVGSTYAPEIVQEVLDTLEIRDVVTVLPQRSHAEALQE
                     MMSADVLFHAMPITRSVEPGGRISAKTYEYLMTD"
     gene            complement(1101..1997)
                     /locus_tag="PKO06_16225"
     CDS             complement(1101..1997)
                     /locus_tag="PKO06_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71252.1"
                     /translation="MQSHLFTGTQPSSSLVLHYSGYGYARHGAPLELVRMLRHWRRTN
                     ANSPIILVFHELYAFSPTPWCASFWLSALQRYVCKLCARIGDRMLTTTSWNRDKLQTL
                     VNVTHNHHTSPVFSTVGESPVCLEQQRRPIAVVFGLQENRCRLYQKHAEALGRWMNEL
                     SIELIVDIGPGDSHVPDILQGRVKPLGCLSIHEIREWLCRCRVGFLDYPVFCLAKSSI
                     FAAYAAHGLVSVVFSEKYPQTDGLIPGRQYLPGHERSVDGHTELQRMSQALKQWYDGH
                     NLDTTVGLLSEWIRDRSAGERH"
BASE COUNT          556 a          638 c          627 g          448 t
ORIGIN      
        1 gtccgtcatg agatactcgt acgtttttgc cgagatccgc ccaccaggtt caaccgatct
       61 cgtgatcggc atggcgtgaa acagcacatc ggcagacatc atctcctgca gagcttcagc
      121 atgagatcgc tgcggcaaca cggtcacgac atctcgaatt tcaagggtgt cgagaacctc
      181 ttgaacgatt tctggagcat aggttgagcc aaccgcaacc aactgaatac ggccgaccgt
      241 ctctggattt cttcggttca cctcatgtat tgcctttccc atcaggcatg gtgtctgcgt
      301 ttctgagcga tgttcagcat actcgaaaag cccggggtgc agaagctttc gcaggagcgc
      361 acgtacggtc gaatatggct gagaacgata gtattcccat cccccggcgt tgccaatgta
      421 cttcatgacc atcgatggtg cgttccctga atcggtcgag cgagacacga aggccgcggc
      481 atcggctccg tatctgatca gatgcaatcg gtgccgatgt tcaggaatcc gcttcgccac
      541 ccgttcaaga ttcgcctgtg agacatacac gacggcatca gcggcggcag gaagatcgtt
      601 ttcgaatcgg cgaaaggcaa ggtagtgcca ccaggtacgg tattggtaga agaagtccgg
      661 atcggtcggc gaatcgtcga gattcgcgat cagaggaact ttctcaacct gcttcagcag
      721 taaccccgca agagcaccgg caaacggcag aacaaaaccg acgaccgcgg caaaacccag
      781 gcgttggtgg agagaacgtg cccggcgaaa cgcacgcaac gcccagacat tcgtccagtc
      841 tggccaacaa agcggccaca gcagaagctc gccaacatgc tgtaatcgcc atttcggcaa
      901 agtctgcacg agagtcgaca cccagctaac aggaagcacc cgatggacgg ttactccagc
      961 aatctgattg ctggcggcgg cggtcgtgat cacgtgcacc tcgtagtcac gggcaagttc
     1021 gcgacacagc ttgccggcgc gaatgcccgt cggccaggca tgcggcggaa aatcggctga
     1081 aatgaccagt attctgggct tcaatgtcgt tccccggcgg accgatctcg aatccactcg
     1141 ctcaatagtc ctaccgttgt gtcgagatta tgcccatcat accactgttt cagggcttga
     1201 gacatccgtt gaagctcagt gtgaccgtct acggaacgtt catgacccgg caggtactgc
     1261 ctgccgggta tcaaaccatc cgtttgtggg tacttttctg aaaagaccac ggagacgagc
     1321 ccatgagcgg catatgcagc aaaaatgctc gacttggcga ggcagaacac cgggtaatcc
     1381 agaaatccga cccgacaccg gcataaccac tcgcgaatct cgtgaataga aagacaacca
     1441 agaggcttca cccgaccctg gagaatatct ggaacatggg aatcaccagg gccgatgtcg
     1501 acgatcagct cgatggatag ttcgttcatc cagcgcccca gtgcttcagc atgcttctga
     1561 tacagtctgc aacgattttc ctgcagtcca aaaacaacgg caatcggtct gcgttgttgt
     1621 tccaggcaca caggcgattc tccaacggtg gaaaagacag gcgacgtgtg atggttgtgc
     1681 gtcacgttca ccagggtttg cagtttatca cgattccaac tcgttgtggt cagcatgcgg
     1741 tcgccgattc tcgcacacag cttgcacaca taacgttgca gggccgaaag ccagaacgac
     1801 gcacaccagg gagtcggtga aaatgcatag agttcatgaa acaccagaat gatggggcta
     1861 ttcgcgttgg tgcgccgcca atggcgcaac atccgtacga gttcaagcgg agccccgtgt
     1921 cgcgcatacc cgtagccgga gtagtgaaga acaaggctgc ttgaaggctg agttccagtg
     1981 aacagatgag actgcatgcg ctgaagactg ccactggagc cgccatgcct gtagaatggt
     2041 cccgagtcgg cacaaaccgg accgcgtaaa aaaacgtacc gagtcagaag ctgctgttgc
     2101 tcatacaacc gtcggccaag gagccaggca tagtcgccga tcccggacgg tacaggcgga
     2161 aaggccggca gcacctgtac acactcgcca atcgatgggt acaacttttc ttcaactgcg
     2221 ctgggtggtt gctttcacgg ctggcgaggg cgagggcgat gccgaccca
//
LOCUS       DAORHD010000851         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_58995_length_2263_cov_3.585598, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000851 DAORHD010000000
VERSION     DAORHD010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58995_length_2263_cov_3.585598"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PKO06_16230"
     CDS             complement(<1..411)
                     /locus_tag="PKO06_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HNV71253.1"
                     /translation="MKFDVNSGIDARRQPCFSLANRTKRALWNIVQATIFGWSPRPAH
                     AWRVFLLRRFGAVIGRECHVYPTARIWAPWNLVMEDQSCLGDDVICYSMATITLGRRA
                     IVSQGAHLCAGTHDFEDPNFPLLAVPITVGPEVWI"
     gene            complement(401..568)
                     /locus_tag="PKO06_16235"
     CDS             complement(401..568)
                     /locus_tag="PKO06_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71254.1"
                     /translation="MSRVCRVFVQSPGWSPAATRLIGGVKIFVGESDQALARSHGAER
                     AGEGWGSRREV"
     gene            complement(565..>2263)
                     /locus_tag="PKO06_16240"
     CDS             complement(565..>2263)
                     /locus_tag="PKO06_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNV71255.1"
                     /translation="ANCQIEDGADLQNVILGDGCVVKAGARLSDSVLWDRVFVGRKVV
                     IDEALIASGTQIRENADIEKGVVISEECRIGEGAVVRQNVKIWPRKQVEDGSTVYTSL
                     VWSEKWTKNLFSNHVITGLANLEITPELASKVGAAFGGTLPKGSSVTTSRDAHRASRM
                     INRAFITGLISSGVQVADLQVTPAPVARYKLSAFGRAGGVHVACSSSDTNQIEIKIFD
                     SEGRDLATGQQKNIERLFNREDFRRVPMMEVGEISFPARVAERYRDELLGRLDLETIR
                     KKHFKVVVDYAFSGASSIFQQVLGKLNCEVIALNSYQQEVPATRSAQETVTALANVAQ
                     IVKSLGADLGLMLDPNAEHLYFVDDRGHPIYGEKSLMAWVSLVFATNPGTRVCVPVST
                     THVVEQIAAKAHGTIIRTKTSSRALMAAATGDKVTFCGDGFGATIFPDFQPAFDAMMS
                     AAKLIEFLARSEKPLSKIVEDLPRFYSETRQIPCPWEAKGAVMRQAMEYAHNHETILI
                     DGVKVVLAKDEWVMILPDPDKPFVNLTVEAGSDNRCRELLEDVQARINTWKGNGGEE"
BASE COUNT          434 a          685 c          700 g          444 t
ORIGIN      
        1 gatccagact tcagggccca cggtaatcgg aacggcgagc aacgggaagt tcgggtcttc
       61 aaagtcgtgc gtaccagcgc acagatgcgc gccctggctg acgatggctc ttcgacccag
      121 ggtgattgtg gccatggagt agcagatgac atcgtcgccc agacacgatt gatcttccat
      181 gaccaggttc cagggggccc agatgcgggc cgtcggatag acatggcact cgcgaccaat
      241 cacggcaccg aagcgccgca gcagaaacac ccgccaggca tgcgccggtc gcggtgacca
      301 gccgaagatc gtcgcttgca cgatgttcca gagggcacgt ttggtgcgat tggccagaga
      361 gaaacacggc tggcggcgcg cgtcaatgcc tgagttgacg tcaaacttca cgccgactcc
      421 cccacccttc gccggcgcgt tccgcgccgt gagatctggc cagcgcttgg tcactctcgc
      481 cgacgaagat cttcacgccg ccgatcaatc gcgtcgccgc cggggaccag cccggtgact
      541 gcacgaatac ccggcaaaca cggctcattc ctcgccgccg ttgcctttcc aggtgttgat
      601 gcgggcctga acgtcctcga gcagctcgcg gcagcgattg tccgagccag cttcgacggt
      661 gagattgacg aatggcttgt cggggtcggg cagaatcatg acccactcgt ccttggccag
      721 caccactttc accccgtcga tcaggatggt ttcgtggttg tgcgcgtatt ccatcgcctg
      781 gcgcatgacg gctcccttcg cttcccacgg gcagggaatc tgacgggttt cgctgtagaa
      841 gcgcggcaga tcttcgacga tcttcgagag cggcttctcg ctgcgggcga ggaactcgat
      901 cagtttggcc gccgacatca tggcgtcgaa cgccggttga aagtcgggaa agatggtcgc
      961 gccgaagccg tcgccgcaga acgtgacctt gtcaccggtt gccgccgcca tcagagcgcg
     1021 cgagctggtc ttggtgcgaa taatcgtgcc gtgcgccttc gcggcgatct gctcgaccac
     1081 atgcgtcgtg gaaacgggaa cacagacccg ggtgccggga ttggtggcaa acaccagcga
     1141 tacccaggcc atcaggctct tttctccgta gatcgggtgg ccacggtcat cgacaaagta
     1201 caagtgctcg gcattggggt cgagcatcag accgagatct gcgccgagag atttcacgat
     1261 ctgcgcgaca ttggcgagcg cggtgaccgt ttcctgcgcc gaacgcgtgg ccgggacttc
     1321 ctgttgatac gagttgagcg cgatgacttc acagttcaac ttgccgagta cctgttgaaa
     1381 aatgctggaa gcgccgctga atgcatagtc aacaacgact ttgaagtgct tcttacggat
     1441 cgtctcgagg tcgaggcggc cgagcagttc gtcgcgatac cgttcggcaa cgcgagccgg
     1501 gaagctgatt tcgccgactt ccatcatggg tacgcggcgg aagtcctcgc ggttgaaaag
     1561 gcgttcgatg ttcttctgct gacccgtcgc gagatcgcgg ccttcggaat caaagatctt
     1621 gatctcgatc tgattggtgt cactcgatga acaagccaca tggacgccgc cggcgcgacc
     1681 gaaggcactg agtttgtacc gggcgaccgg agccggagtg acctgaagat cagcaacctg
     1741 gacccccgaa gaaatcaagc cggtgataaa cgcgcggttg atcatgcggc tggcgcgatg
     1801 ggcatcgcgt gaggtggtca cgctcgaacc tttgggcagc gtgccgccga aggccgctcc
     1861 gaccttggag gccagttcgg gcgtgatttc gagattggcg aggccggtaa tgacatggtt
     1921 cgagaagagg ttcttcgtcc acttctcgct ccagaccagg ctggtgtaga ccgtcgagcc
     1981 gtcttcgacc tgcttgcgcg gccagatctt gacgttctgg cgcacgaccg ccccttcgcc
     2041 gatgcggcat tcctcgctga tgacgacccc tttttcgatg tcggcatttt cacgaatctg
     2101 cgtcccgctg gcaatcagcg cttcatcgat gacgaccttg cgacccacga agacgcggtc
     2161 ccagagcaca gagtccgaga ggcgggcccc ggctttgacc acgcagccgt cacccagaat
     2221 gacgttctgc agatcggcac cgtcttcgat ctggcagttc gcg
//
LOCUS       DAORHD010000852         2263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59003_length_2263_cov_3.070199, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000852 DAORHD010000000
VERSION     DAORHD010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59003_length_2263_cov_3.070199"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PKO06_16245"
     CDS             <1..436
                     /locus_tag="PKO06_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71256.1"
                     /translation="SQAAQFFDSVAQVREEASAEADAVELPTPESLPAALPDSSATLD
                     SSFPLGYIPLADGSGMEFPARDYKSFLNNFLKYVEQFRKNMMKDFLAGLPRPVAYGKP
                     VESPTSPAKTPGAAPAIVPDTSEPVAVTTDQSAIFPTGEDPA"
     gene            complement(550..870)
                     /gene="trxA"
                     /locus_tag="PKO06_16250"
     CDS             complement(550..870)
                     /gene="trxA"
                     /locus_tag="PKO06_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV71257.1"
                     /translation="MAKELNETMFKAEVVDAKAAAMIDFWAPWCGPCRVMGPLVDNLS
                     KKYDGKLVVAKVNVDDNQNLAGQFNIMSIPTILFFKGGKVVHTHVGSTTEADLENLLK
                     QHLL"
     gene            complement(918..1088)
                     /locus_tag="PKO06_16255"
     CDS             complement(918..1088)
                     /locus_tag="PKO06_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HNV71258.1"
                     /translation="MSTVLAKAIAPATGAVLGFLYYKFIGCATGACPITSNPVISTLY
                     GALIGLALSFGK"
     gene            complement(1085..1321)
                     /locus_tag="PKO06_16260"
     CDS             complement(1085..1321)
                     /locus_tag="PKO06_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNV71259.1"
                     /translation="MVIERIIRIMAGSFVMASVILSQVHHPYWLLFTLFVGANLFQSG
                     ITRWCLMEDILKKLGFRSCCTTDGSVSRKEAHAS"
     gene            complement(1575..2207)
                     /locus_tag="PKO06_16265"
     CDS             complement(1575..2207)
                     /locus_tag="PKO06_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71260.1"
                     /translation="MRVIILEGITGSGKTTLLRMLARLIAERWRGPVWLATEHVTERV
                     LEPLAQATPARARNQLRQHLMHLKRLQAWDAEAPPGDCTETLAVLERFHLSAVLHITG
                     MTARDFSRTENALPVFHPLLVWCELTEAEVLERSVRLTSQMRGERWNQYLRTLGANET
                     EWFRHFTSEQQRLAEWFERSSLRKCRIHVEDATVAMRAAELFDTMIQPTL"
BASE COUNT          516 a          677 c          573 g          497 t
ORIGIN      
        1 aagccaggcg gcccagttct tcgacagcgt cgcccaggtt cgtgaagaag cgtcggccga
       61 agccgatgcc gtcgaactcc ccacacccga aagcctgcct gccgcgctgc ccgacagctc
      121 tgccacactc gactcgagct tcccgctggg atatattccc ctggctgacg gtagcggtat
      181 ggagtttccg gcccgtgact acaagagctt tctgaacaac tttctgaagt atgtcgaaca
      241 gttccgtaag aatatgatga aggatttcct cgccggcctg cctcggcccg tcgcctatgg
      301 gaaacctgtg gaatcgccga cttccccggc gaagactcct ggtgcagccc cggcgattgt
      361 tcccgacacc agcgagccgg tcgcggttac gaccgaccag tctgcgattt ttcccaccgg
      421 agaagatccg gcgtaagaca gcgtttcctc cagaatcgcc cgtcgaaaga ttgatgatga
      481 aaagggcacc cgatcagggt gccctctttt tctgtctggc gaatcaccga ctcaacgtcg
      541 gcgctcacat cacagaagat gctgcttgag caggttctcg agatcggctt cggtcgtcga
      601 accaacgtgg gtatggacga cttttccgcc cttgaagaac agaattgttg gaatggacat
      661 gatgttgaac tgaccagcca gattctggtt gtcatccaca tttaccttgg ccacgacgag
      721 ttttccatca tacttcttgg aaagattgtc aaccaaaggc cccatcaccc ggcacggacc
      781 gcaccaggga gcccagaaat cgatcatggc tgccgctttc gcgtcgacaa cctctgcttt
      841 aaacatcgtt tcgttcaatt ctttcgccat ataacctcct gacaaatgcg ttgatagtgc
      901 tttgactgtt taccgtacta cttgccaaat gacaaggcca aaccgatcag tgccccgtag
      961 agcgtgctga tcaccggatt cgaggtaatc gggcaggcgc cggtcgcgca accgatgaat
     1021 ttgtaataga ggaagcccag tactgccccg gttgccggag cgatcgcttt tgccaacacg
     1081 gtactcatga tgcgtgtgcc tcctttctcg agacggagcc atcggtcgtg cagcaggatc
     1141 tgaagcccag cttcttcaag atgtcctcca tcagacacca tcgagtgatc cctgattgaa
     1201 agaggttcgc gccgacaaac agcgtgaaga gcaaccagta tgggtgatgg acctgcgaca
     1261 agatgacact cgccatgacg aaactccccg ccataatacg aatgatacgt tcgataacca
     1321 tttcgtcctc ctcgattcaa ttccgtgttc ccgggggaac aatgcgttca cgcgtctaat
     1381 cagcaacagg cgtgccaagt aaaagcaaag aacctgtcac aagcctccat acagacaaca
     1441 cgaggcatct ttcacagaac cgactcacgg aacaccacct caatcatcac tgtttacatg
     1501 caagaacttc acgttttcat gcattatttg cgtattcatt acctcaagag aagcgcactt
     1561 cgtctgcgaa agaatcaaag cgttggctgg atcatcgtgt caaaaagttc cgccgcccgc
     1621 atcgccaccg tcgcgtcctc tacgtgtatg cggcatttgc gcagcgagct gcgttcgaac
     1681 cattcggcta aacgctgctg ttcagacgtg aagtgcctga accattccgt ctcattcgct
     1741 cccagggtcc gcagatactg attccagcgt tcgccacgca tctgagacgt caggcgcacg
     1801 ctccgttcca gcacctccgc ctcggtcagt tcacaccaga cgagcagcgg gtgaaacaca
     1861 ggcaacgcgt tttcggtacg ggagaaatcg cgagcggtca ttcccgtgat gtgaagcaca
     1921 gcgctcaggt ggaatcgttc gagcaccgca agagtctccg tgcaatcgcc tggcggggct
     1981 tcggcatccc aggcctgaag tctcttgaga tgcattagat gctgacgcaa ctgattgcgc
     2041 gcccgagccg gtgtcgcctg agccagaggt tcgagcacac gctcggtcac atgttcggtc
     2101 gctagccaga cggggcctcg ccaacgctca gcgatcaatc gggcaagcat acgcagcaag
     2161 gtcgtctttc ccgatccggt gatcccttcg aggatgatga ctctcatgtt ggtcttccct
     2221 tcgcatcaag accggacaag aagcttttct tgcctgatcc att
//
LOCUS       DAORHD010000853         2260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59128_length_2260_cov_4.196825, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000853 DAORHD010000000
VERSION     DAORHD010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59128_length_2260_cov_4.196825"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKO06_16270"
     CDS             <1..714
                     /locus_tag="PKO06_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71261.1"
                     /translation="SKSVSNFSGVSDLYFIWLVAALIQIPVIIRGWKQRAIPNSIPND
                     LVGDEAPLYYALFLFPFLIVPIQTIANVYPDAIAANSALNNPAFIIPYLVLGLFFLDA
                     FCSDQIEEAVGRNNFHFWGLMAIMVLALVAGGNLQFFSDLKLTVWDANKGLIIAGHLL
                     VGIGRGNVMSLGFIGVVVVYHLIGFMKSIAQNVYDQTKDLSLVQWASMLMGASFVFLG
                     LGFWVSVKGAKSRRNKRPE"
     gene            711..1157
                     /gene="ruvX"
                     /locus_tag="PKO06_16275"
     CDS             711..1157
                     /gene="ruvX"
                     /locus_tag="PKO06_16275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNV71262.1"
                     /translation="MKFLALDVGQRRIGVASCDRFEMTVRPLQVIRAGEQAARELRML
                     CEREEAEGIVVGLPLSTDGTERDACERVRVFVDRLKTCTSLPIYLEDERHTTQFAAGR
                     LADEGVRKEKRRDLIDAWAAAEILELFLAGRRKRGETYDQQPEPVT"
     gene            1273..1680
                     /locus_tag="PKO06_16280"
     CDS             1273..1680
                     /locus_tag="PKO06_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71263.1"
                     /translation="MSHPTYRCVLICEDIRHETGGRVSLMGVLGSKLYVQDFPLFFPK
                     LCLFIEWGEITGRVMVTLNIVAPEGVTLPKVQPKAEIRGQAGIVARSMIVLNSFVFPK
                     GGDYVFQFLIDDQIVGQETLRVEKFEQPPSLTN"
     gene            1689..>2260
                     /locus_tag="PKO06_16285"
     CDS             1689..>2260
                     /locus_tag="PKO06_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71264.1"
                     /translation="MPVPASGNASTAFLQVLQQAIAHPQSLRAMIEPYLVQDADGCIP
                     HLIQVFDTTEMAAKRAIADVFLGSLRAKAAEALLPYLGQTNVDRYFWAAEVLGEMLEA
                     RALPFLIKGLAGTHKSVVIASVKGVARFRSEESFSALIKFFNSCRDEVFLSASLRFLV
                     PLKDDLVPRLLPTFSAQDRFRKAWLLKYFA"
BASE COUNT          441 a          609 c          658 g          552 t
ORIGIN      
        1 tcaaagagtg tgtcgaattt ttccggggtc tcagatctgt atttcatctg gctggtcgcg
       61 gcgctgattc agattcctgt catcatccgc ggctggaaac aaagagcaat tccaaactcg
      121 attcccaatg atctggtcgg tgacgaagcg ccgctctact acgcgctctt cctgtttccg
      181 tttctgatcg taccaattca aacgatcgca aatgtttatc cggacgcaat agcagcaaat
      241 tcggctctga acaaccctgc attcatcatt ccctatctgg tactggggct gttctttctc
      301 gatgccttct gcagcgatca gatcgaggaa gcggttgggc gtaacaactt tcacttctgg
      361 ggtctgatgg ccatcatggt tttggctctg gtggcgggcg gcaatctgca gttcttctcc
      421 gatctgaagt tgaccgtctg ggatgcaaac aaggggttga tcatcgccgg acatctgctg
      481 gtcggtatcg gccgcggaaa tgtcatgagc cttggtttta tcggcgtggt cgtcgtgtat
      541 cacctgatcg ggttcatgaa gtcgattgcc cagaacgtct acgatcagac gaaagacctg
      601 agcctggtcc agtgggcatc catgctgatg ggggcgtcat tcgtgtttct cgggctcggc
      661 ttctgggtca gcgtcaaggg agcgaaaagt cgccgcaaca aacgcccgga atgaagtttc
      721 tggctctgga tgtcggtcag cgtcgtatcg gggtcgcttc ctgcgaccga ttcgaaatga
      781 cggtgcggcc gctgcaggtc atccgggctg gagagcaggc cgctcgcgag ctgcgaatgc
      841 tctgcgaacg agaagaagcc gaggggattg tggtcggctt gccgctctcg actgatggca
      901 cagagcgtga cgcctgcgag cgagttcgcg tgttcgtcga tcgcttgaag acctgcacct
      961 cacttcccat ctatctggaa gacgaacgcc acaccacgca gttcgccgcc ggtcgactgg
     1021 ccgacgaagg agtgcgcaag gaaaagcgac gcgatctcat cgatgcgtgg gctgccgcgg
     1081 agattctgga actgtttctg gccggtcgtc ggaaacgggg cgagacctac gatcagcagc
     1141 cggagcctgt cacatgaacg gctctgcggc gttgtcgaga atgcgggaaa ggaagtctta
     1201 cgcgggggta gccgctgccg ggtcgttctg gtacaatgcc tgcgaaatcc gttcacggaa
     1261 aaaaggaatc acatgtcaca tccaacctat cgctgtgttc tgatctgcga agacattcgt
     1321 cacgagacag gtgggcgggt cagcctcatg ggcgtcctgg gctcgaaatt gtacgttcag
     1381 gattttccgc tgtttttccc gaaactctgc ctgttcatcg agtggggtga aatcaccggg
     1441 cgtgtcatgg taacgctgaa tatcgttgcg cccgaggggg tgaccttgcc caaggttcag
     1501 ccgaaagcgg aaattcgcgg gcaggctgga atcgtggcac ggtccatgat tgttctcaac
     1561 agcttcgttt ttcccaaagg tggagactac gtcttccagt ttctcatcga cgaccagatc
     1621 gtgggtcagg aaaccctgcg ggtcgagaaa ttcgaacagc cgccgtcgct gaccaactga
     1681 tttctcctat gcctgttccc gcttccggaa acgcttccac cgcttttctg caggtactcc
     1741 agcaggcgat tgctcatccg caatcgttgc gcgccatgat cgagccgtat cttgtccagg
     1801 atgccgatgg ctgtatccct catttgatac aggtttttga taccacggaa atggccgcga
     1861 agcgggcgat tgcggatgtg tttcttggct cgctgcgcgc caaagcggct gaagccttgc
     1921 tgccctacct cggccagacg aatgtcgatc ggtatttctg ggccgctgaa gtactgggtg
     1981 agatgctcga agcgcgcgct ctgccgtttc tcatcaaagg gttggccggc acgcacaagt
     2041 ccgtggtgat cgcttcggtg aaaggcgttg cccgctttcg ctcggaagag tcattttctg
     2101 ccctgatcaa gtttttcaac agctgtcgcg acgaagtatt tctgtcggcc tctctgcgat
     2161 tcctcgttcc tctcaaggat gacctggtac cgcgtctgct gccaacgttt tctgcacagg
     2221 accgctttcg caaagcctgg ttactgaaat actttgcgga
//
LOCUS       DAORHD010000854         2259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59187_length_2259_cov_3.389292, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000854 DAORHD010000000
VERSION     DAORHD010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59187_length_2259_cov_3.389292"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="PKO06_16290"
     CDS             <1..977
                     /locus_tag="PKO06_16290"
                     /inference="COORDINATES: protein motif:HMM:NF018504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase PrkA"
                     /protein_id="HNV71265.1"
                     /translation="DHFGRIIVSSRIKKDSKAIKDWIDDDDDYEKCCDPDFLLLKMEL
                     YSGRIPDWLTKTDRNNLKADVWKSILAEAETEGFEGFSGRESNQVFDEFYSKYSKKRP
                     INMRHVAEFFNDDKKSYKDKLPRNFIKHLTDLYDFVVLKEVKDSMYDYNEDEISKTIL
                     NYLVAITANIGDVVKNPWNNGEEITVTADFLDIVETHLKDPDASQYAKERYRKEAVDE
                     YASVTLAREIKTEGKKITETKQYLDMFAAFTKSARQRVLEPYIANSNFRLAIKEFGLE
                     DFQKYDTKIRDKVQLLFHNLHIKYQYNQECARAACLYVLDNNLVEKFK"
     gene            1082..1969
                     /locus_tag="PKO06_16295"
     CDS             1082..1969
                     /locus_tag="PKO06_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV71266.1"
                     /translation="MMHREAFEKNRSVMLVIDPTDGAIRDANPAAAAFYGYSREALCN
                     MNITQINTLPHDRVMREMQDALTECQGVFSFQHRLANGETRDVEVHSSPFAVDGQTLL
                     FSIVVDISERRRNEEALILQHRLAAALAQSASLQESLEACLMTALQLSGMDSGGVYLR
                     EERTGEMVLTVHRGLSNEFVTWAARFTPDAPEYRQIGTGTPIYSTYAHSTNPVHRREG
                     LCAVAAIPIKHQGQVTACLNIASHTRAHIDERCWIPLETVVSQMGSFVARALLLEKLR
                     VSEERYRTLFQQMAQGMIR"
BASE COUNT          532 a          674 c          609 g          444 t
ORIGIN      
        1 tcgatcactt cggccgcatt atcgtctcgt cacgcatcaa gaaagactcg aaggccatca
       61 aggactggat cgacgacgac gatgactacg agaaatgctg cgatcctgac tttcttttgc
      121 tgaaaatgga gttgtactcc ggccggattc ccgactggct gaccaagacc gaccgcaaca
      181 atctcaaggc cgacgtctgg aagagcattc tggccgaagc cgaaaccgaa ggcttcgagg
      241 gcttctcggg gcgcgagtca aaccaggtat tcgacgagtt ctattccaag tattcgaaga
      301 agcgaccgat caacatgcgc cacgttgccg aatttttcaa cgacgacaag aagagctaca
      361 aggacaaact gccgcgcaac ttcatcaagc atcttaccga cttgtacgat ttcgtcgtgc
      421 tgaaagaagt gaaagactcg atgtatgact acaacgagga cgagatcagc aagacgattc
      481 tcaattatct cgtggccatt accgccaaca tcggcgacgt ggtgaagaac ccctggaaca
      541 atggcgaaga gatcaccgtc acggccgact tcctcgacat cgtcgaaacg catctgaaag
      601 accccgacgc gtcgcagtat gccaaagagc ggtatcgcaa agaggccgtc gacgagtacg
      661 cgtctgtgac gctggcccgc gagatcaaaa ccgagggcaa gaagatcacc gaaaccaagc
      721 agtatctcga catgttcgcc gccttcacga aatcggctcg tcagcgcgta ctcgaaccct
      781 acatcgccaa ctccaacttc cggctcgcca tcaaggagtt cggcctcgaa gactttcaga
      841 agtacgacac gaagatccgc gacaaggtcc agttgctgtt ccataacctg cacatcaagt
      901 accagtacaa tcaggaatgc gcccgcgccg cctgcctcta cgtcctcgac aacaacctcg
      961 tcgaaaagtt caagtagatc gagcgtggat ccatttcagg agtcgcacga ttcggctcac
     1021 cgccattggc ggtctgcagg gccagagcca cgctgagacg aaatcagcac ggggtttacc
     1081 gatgatgcat cgcgaagcgt ttgagaagaa ccggtctgtc atgctggtga tcgacccgac
     1141 ggatggggcg attcgcgatg ccaaccccgc cgccgccgcg ttttacgggt attcgcgcga
     1201 agcgctctgc aacatgaaca tcacgcagat caacaccctg ccgcacgacc gcgtgatgcg
     1261 tgaaatgcag gatgccctga cggaatgtca gggggttttc tcgtttcaac atcgcctggc
     1321 gaacggagag acccgggatg tcgaggttca ctcgagcccg tttgcggtcg acgggcagac
     1381 gctgcttttc tcgatcgtcg tcgatatctc cgagcgcagg cgcaacgagg aagcgctgat
     1441 tttgcagcac cgtctggcgg ctgctcttgc ccagtcggcc tctctgcagg agagcctcga
     1501 agcctgtctg atgacagccc tgcagctgtc aggcatggac agcggcggtg tctacctgcg
     1561 cgaggaacga accggcgaaa tggtgttgac cgttcaccgg gggctttcca acgagttcgt
     1621 cacctgggcc gcgcgcttca cccccgacgc gccagagtac cgacagatcg gcactggaac
     1681 ccctatctat tcaacgtatg cacactcgac caatcctgtg catcgacgtg aaggtctctg
     1741 cgcggtggct gccattccca tcaaacatca ggggcaggtg acggcctgtc tcaacatcgc
     1801 gtcgcatacc cgtgcacaca tcgacgaacg ctgctggatc ccactcgaaa ccgtcgtttc
     1861 gcagatgggg tcgtttgtcg cccgagctct gctgctcgag aagcttcgcg tcagtgaaga
     1921 acggtaccgc accctctttc agcagatggc tcagggaatg atccggtaac tccccggccc
     1981 cggcacaaga agcctttctc gcccatcatc tcggccgcgc gcttcacggt cgaagcggga
     2041 attcctgatt ggttgttttc tgatgattcc atgttatgat tgtccacttc aggtgtgtga
     2101 caacgcacgt ttgacgtgag caagcatgag aggccagacc aatatgtcgg tcagtgaacg
     2161 acttcaaacc ttacttcacg aactccagaa cgagccgggc aacgatgcct cgtcgccagc
     2221 agctcaggaa cgccgctcga acctgttaat gcgcgcggc
//
LOCUS       DAORHD010000855         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59257_length_2257_cov_4.215259, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000855 DAORHD010000000
VERSION     DAORHD010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59257_length_2257_cov_4.215259"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKO06_16300"
     CDS             complement(<1..633)
                     /locus_tag="PKO06_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71267.1"
                     /translation="MSHVRVIDPPHPRGRLLIIDDDPDFAQVIREMLAPHGYEVEIAT
                     DRSVGRRLASSGDHDLIIIDAILESSIEGIELAHEFRSMASLRHKPLLLISTMTASSQ
                     FPQQFAAMLDREWPFDGYLEKPVDQTRLLRFLDHLIPTDPKRAAQRAATRPLVLVVDD
                     DPDQQTIARETLGPAGLEVACVFNSYEAREWLKTHHAQLILLDVMMSTPTE"
     gene            complement(633..>2257)
                     /locus_tag="PKO06_16305"
     CDS             complement(633..>2257)
                     /locus_tag="PKO06_16305"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNV71268.1"
                     /translation="LIASPCPVVVSLIERHYPHLIKFLMPTVSPLVATGRYLLENDPT
                     PSIKVFIGPCIAKKEEIQDPQLKGVIDHALTYREVKEMFKARAIDPAKLHGMPADPPH
                     PGQGRVFPLVGGILKTAGMGTDILDDRIAIIEGHHETLEFLRTFDENHPPRCDFVDIL
                     FCRGCIDGVEMDSELSHLDKHRKVCEFVSESRHLNPSSLPEVNLKRPYQDRKLRTMEP
                     TAEEITEILRMFGKHKPEDELNCGACGYRTCREKARAVFQGIAEPEMCLPEIIKQLEK
                     TVVDLNISHEELKKAQDELVHSEKLASLGQLSAGVAHELNNPLGGILLFSNMLQEKLA
                     QIGKDTLPEEIDIIVKEATRCRNIVRGLLDFARQSKLQKTRIDIGELLAEISTIERRA
                     APQGVQFVEEYDPALPPTMLDPVQIRQALTNIIHNAVEAMPQGGTLKIVTRHSALTQT
                     AEITIADSGVGIPDNSISKLFTPFFTTKGVGKGTGLGLAICYGIIKMHRGSINVQSSV
                     GKGTTFNISLPLESPPEETVIGGPAPHGSGGR"
BASE COUNT          436 a          601 c          706 g          514 t
ORIGIN      
        1 ttccgtcggg gtgctcatca tgacgtcaag cagaatgagc tgggcgtggt gagttttcag
       61 ccattcgcgt gcctcgtagc tgttgaagac gcaggcgact tcaagaccgg ccggtccgag
      121 cgtttcacgg gcgatcgtct gctgatccgg gtcgtcgtcg accacgagca cgagcgggcg
      181 agtggcggct ctctgggcag cgcgcttcgg gtcggtcgga atcagatggt cgaggaaacg
      241 caacaacctg gtttgatcca ccggtttttc caggtaaccg tcgaacggcc attcgcgatc
      301 gagcatagcc gcgaactgtt ggggaaactg tgatgaggcg gtcatggtgg aaatcaacag
      361 caggggcttg tgtcgcagcg atgccattga gcggaactcg tgagccaatt cgatgccctc
      421 gatcgatgat tccagaatgg cgtcgatgat gatcagatcg tgatcaccgc tggaagccag
      481 tcttcgcccc acgctgcgat cggtcgcgat ttccacttcg tagccgtgag gggcgagcat
      541 ttcgcgtatc acctgcgcga agtccggatc atcatcgatg atcagcagcc tgccacgagg
      601 atgaggtgga tcaatgactc gtacgtgcga cactaccgac ctccgctgcc gtggggtgcc
      661 gggccgccga tcacggtttc ttcaggtggg ctttcgagcg gcaacgaaat attgaaggtc
      721 gttccctttc cgacggaact ctgcacattg attgaaccgc gatgcatctt gatgatgccg
      781 taacagatgg cgagtccgag gccggtgcct ttcccaacgc ctttggtggt gaagaagggc
      841 gtaaacaatt tactgatgct gttgtccgga attccgaccc cactgtcagc gatcgtaatc
      901 tctgccgtct gcgtcagcgc actgtgtctg gtgacgatct tcagcgtgcc gccctgcggc
      961 atggcctcga cagcgttgtg aataatgttg gtgagggcct gacgaatctg caccgggtcg
     1021 agcatggtcg gcggcaatgc gggatcatac tcttcaacaa actgtacgcc ctgcggggcc
     1081 gcccgtcgct cgatggtgga gatttcagcc agcaattcgc cgatgtcgat gcgggttttc
     1141 tgcagcttgc tctggcgagc gaaatcgagc aggccacgca cgatgttccg gcatcgggtg
     1201 gcttccttga cgataatgtc gatctcttca ggcaatgtgt ctttgccgat ctgggcgagt
     1261 ttttcctgca gcatgttcga aaacagcaga atgccgccga gcgggttgtt gagttcgtga
     1321 gcgacgccgg ctgacagctg gccgagagat gcgagtttct ctgagtgtac cagttcgtcc
     1381 tgggctttct tcagctcttc atgagaaatg ttgaggtcga caactgtttt ctcgagctgc
     1441 ttgatgatct cgggcaggca catctcgggt tcggcaattc cctggaatac ggctcgggcc
     1501 ttttctcgac aggtacgata cccgcaggcg ccgcagttca gttcgtcctc gggtttgtgc
     1561 ttgccgaaca tgcgcagaat ctcggtaatc tcttcggccg tcggttccat ggtgcgcagt
     1621 ttgcggtcct gatacggacg cttgagattg acctcaggga gcgaagatgg gttgagatgg
     1681 cgcgattccg agacgaattc gcaaaccttg cgatgtttgt ccaggtgact cagttctgaa
     1741 tccatctcga cgccgtcgat acagccacgg cagaacaaaa tatccacgaa atcgcagcga
     1801 ggcgggtggt tctcgtcgaa ggtgcgcagg aattcgaggg tttcgtggtg gccttcgatg
     1861 atggcaatgc gatcgtcgag aatgtcggtg cccatgccgg ccgttttgag aatgccgcca
     1921 acgagcggaa agacgcgccc ctggcccggg tgcggcgggt cggccggcat gccgtgcagc
     1981 ttcgccggat caatggcacg ggccttgaac atctctttga cttcacgata ggtcagggcg
     2041 tgatcgatga cccctttgag ctggggatcc tggatctctt cctttttcgc gatgcagggg
     2101 ccgataaaca ccttgatcga gggagtcggg tcgttttcga gcagataccg gccggtcgcg
     2161 accaggggcg agacggtggg catcagaaac ttgatcagat gaggataatg ccgctcaatc
     2221 agactcacga cgaccggaca cggactggcg atcagcg
//
LOCUS       DAORHD010000856         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59269_length_2257_cov_3.153951, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000856 DAORHD010000000
VERSION     DAORHD010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59269_length_2257_cov_3.153951"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="PKO06_16310"
     CDS             <1..541
                     /locus_tag="PKO06_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV71269.1"
                     /translation="LLLVRKDSGISSLADLQGKGLIIEKLATGGLPQIWIDVLLHRQG
                     LPAHTRHFGSVKLVDKPSAAAMPVFFGQTTACIVRESGYKTIVEMNPQIDQTLKVLER
                     SPECLRALALYRADYPEEDRKRLNRSAIKLHNSVKGQQILTLFKTEKIIPYDPSFLKT
                     TEALLKEYESILQPRSKGK"
     gene            complement(585..1583)
                     /locus_tag="PKO06_16315"
     CDS             complement(585..1583)
                     /locus_tag="PKO06_16315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV71270.1"
                     /translation="MSHFGPPSDKPDTEKVSLTSVPPLGGAWFVALCGMLRSTDLFFR
                     IGALKALSTLTLIAWEHLVNAVLVLPLAWRNRRYYRQVTRTEMLLFLLIGVGASACGI
                     LFFTAAFRRLNPALVILLQKLQPLITIGLGIAVLKEQPRRHFWLFAGLAIASSYFVTF
                     SLSNPFQVEWWQLASGAGYAVAAAFFWGGGTAWGKLLLGKYPQSFLLANRFLLGAVFT
                     VMLAMLTGRGLRYDLVVTGTEPLLDDIAYMALIPGLLATAAFYHGLNRLPAAITSILE
                     LIFPLSSVIIMWVWFDRPIDGVQIAAAMILFWSMYMVSRDPEMSTSQAVDNSAKRP"
     gene            complement(1708..>2257)
                     /locus_tag="PKO06_16320"
     CDS             complement(1708..>2257)
                     /locus_tag="PKO06_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71271.1"
                     /translation="ADPAAVELTNEEQEAILKIQEMLGLPTARVVLSVPQIEAILACG
                     RALQKELKPIPVKDVILDPGEKAFFEFTADLLEITSGGGVRLHFPPEFYLDVFRGIIT
                     SSPPAQKIETGRLIITSRRVLFVGSNRTLAMSYDLITGMDLFIDLLRINSVGKDRVCV
                     MGVENPPLVALAISYAYRDGTG"
BASE COUNT          567 a          656 c          599 g          435 t
ORIGIN      
        1 actgctcctg gtacggaaag actctgggat ctcctctctc gccgaccttc aaggaaaagg
       61 gttgatcatc gaaaaactgg caaccggtgg gttaccgcaa atctggatcg atgtgctgct
      121 gcatcgccaa ggacttccgg cgcataccag gcattttggc agtgtgaagc tggtcgacaa
      181 accctctgct gccgccatgc cggtcttttt cggccaaacc accgcctgta tcgtgcgcga
      241 gagtgggtac aagacgatcg tcgagatgaa cccccagatc gaccaaaccc tgaaggtgct
      301 cgaacgttca ccggaatgcc ttcgggctct cgcgttgtat cgcgccgatt atccggaaga
      361 ggatcgaaaa cggctgaatc gatctgcgat caagctgcac aactcggtga aggggcaaca
      421 gatcttgacc ctgttcaaaa cagaaaagat cattccctat gatccttcat tcctgaaaac
      481 gactgaagcc ctcttgaaag agtacgagtc gattctccaa cccaggtcca aagggaagta
      541 gtcgctccag ccctgagtcg gacgactggt actgtacgag ttgatcaggg tcgcttcgct
      601 gaattgtcaa cagcctgaga agtcgacatc tccggatctc gcgacaccat atacatgctc
      661 cagaagagaa tcatcgctgc tgctatctgc acgccgtcga ttggacgatc gaaccatacc
      721 cacatgataa tcacggaaga aagcggaaaa atcagttcca gaatcgaggt gatcgccgcc
      781 ggcagacgat tcagaccatg gtagaatgcc gccgtcgcca gaagtccggg aatgagtgcc
      841 atgtaggcaa tatcatccag aagcggttcc gtaccagtca cgacaagatc ataacgcagg
      901 cctctgccgg tcagcatggc tagcattacc gtgaaaactg ctccaagcag aaaccggttc
      961 gccagcagaa atgactgcgg atattttccg agcaacagct ttccccaggc tgtcccacca
     1021 ccccagaaaa acgcggcggc aaccgcatac ccagctcctg aagccaattg ccaccactcg
     1081 acctgaaagg ggttggacaa tgaaaatgtg acaaagtaac tgctggcaat cgccagcccg
     1141 gcgaacagcc agaaatgacg tcgcggctgt tccttgagca cggcgatgcc gagaccgatg
     1201 gtgatcaagg gctgcagttt ctgcaacaga atcaccaaag ccggattgag tcggcgaaaa
     1261 gccgccgtga agaataggat cccgcatgcc gaggcaccaa cgccgatcag aagaaacagc
     1321 agcatttccg tgcgcgtcac ctgacgatag taccgtcggt ttcgccacgc caggggaagc
     1381 accagcaccg cgttcaccag gtgttcccag gcgatgagag tcagtgtcga aagtgctttc
     1441 agagctccga tacgaaaaaa caaatcggta ctgcgcaaca ttccgcaaag agcgacgaac
     1501 cacgctccgc cgagcggcgg cacagaagtg agagaaacct tttccgtgtc cggcttgtcc
     1561 gatggtggtc cgaagtggct catgcgcgtc agagactaca gccgcatgca tctgtgcgtc
     1621 aagacttcca ttgccttcgt tcgatgccac gcaccgtcac gaaggccctg actgttcatt
     1681 caacggagcg tttcgcttct ggtgcgctca acccgtgcca tcgcgatacg cgtaactgat
     1741 cgccagcgcc accagcgggg gattctcgac acccatgacg cacacccggt ctttgcccac
     1801 cgagttgatg cgcagcagat cgataaacag atccatgccg gtgatcagat cgtaactcat
     1861 cgccagagtg cgatttgaac cgacaaacaa cacgcggcgc gaggtgatga tcaggcgacc
     1921 agtctcgatc ttctgtgccg gcggactgga ggtgatgatg ccccgaaaca cgtcgaggta
     1981 gaattccggg ggaaagtgca gccggacccc gcctcctgaa gtgatctcga gcagatcggc
     2041 ggtaaactcg aaaaaggctt tctcgcctgg gtcaagaatc acatccttga ccggaatcgg
     2101 tttcagttcc ttctgcaaag ctcgaccaca cgccaaaatg gcttcgatct gcggcacaga
     2161 cagaaccacc cgtgccgtgg gcagacccag catttcctga atcttcaaga tggcttcctg
     2221 ctcttcgttc gtcagttcga ctgcggcggg gtctgcg
//
LOCUS       DAORHD010000857         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59481_length_2252_cov_3.705963, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000857 DAORHD010000000
VERSION     DAORHD010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59481_length_2252_cov_3.705963"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKO06_16325"
     CDS             <1..409
                     /locus_tag="PKO06_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71272.1"
                     /translation="AEPDLSGDTSDSIRYWRGVQVTKMPDGTVQKSPANLMSCHNHAR
                     NEHYLKDVDTGEIFGRGKSKYHYTYKNGNHHLAINDRSQPSMTQDAVIDFFETEDTLT
                     GKGTSNVELRDPSSGRVTRQSLSFSYQLKRVKN"
     gene            complement(446..2047)
                     /locus_tag="PKO06_16330"
     CDS             complement(446..2047)
                     /locus_tag="PKO06_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71273.1"
                     /translation="MFSFLRFRWKLWIVILGIPYYLIFLTIEPPKAFQISPETTYFTS
                     PLTPSGRVDYGQALNLRYGPVSPADNVVIPLLRLYSPASLPIGTEAAAIASLLGTPFP
                     LPADLSYRPLDEFLASSAVSTPEVVSGTRDAASAAFLAASLTPRPIDAEFPQPPVDIG
                     KVRLKECGCKPWKASEYPDVDSWINANQQSFALLADASQRKRFWWPFLQGDSGDDLLA
                     GKLFGRSHYVQAHRGLLARSMRRLGTGDTDGSLSDLQIALRLSRHVTETDFLVDKLLG
                     LLTLDETLKTCEAWITIASLSPKLCDQLADSLSVLRPIPSMNNALETEHLWQFVLLYN
                     QAYDKNHAVQQYVDFNQLARRFLQLKASHSKILSDPDLKRKLETLTVWSTQPNSFDWL
                     DDKNQLIWRIVKVILFTTRWTKVSAITDLYGDVVLSIAVPNYSELIRHSLTVAVRLDL
                     VHLAALLKKAQITTGTFPPRLEMILPDAASIPIDPFSGKPLIYRYENNGCLMYSYGHN
                     FADDGAASDTSATTRSDDIVVRLAP"
BASE COUNT          570 a          536 c          639 g          507 t
ORIGIN      
        1 tgcagaacct gacttgtcgg gagacacgag cgatagtatc cgatactggc gtggagtgca
       61 ggtaacgaag atgcctgatg gcacggttca gaaatcacca gccaacttga tgagctgtca
      121 taaccatgcc agaaacgaac actatttaaa ggatgtagat acgggcgaaa tcttcggtcg
      181 cggcaaaagc aagtatcact atacgtacaa gaacggaaat catcatctgg cgatcaatga
      241 ccgttctcag ccatctatga ctcaagacgc cgtcattgat ttctttgaga ccgaagatac
      301 tctgaccggc aaagggacct cgaacgtcga actgcgcgac cccagctctg gccgcgtcac
      361 caggcagtcg ctgtccttca gttaccagct caaacgagtg aaaaactagg cgtcagaaaa
      421 cctgtcggat cttcagatag atgtgtcagg gagcgagtct gacgacgatg tcgtctgatc
      481 gcgttgtcgc tgaggtgtcg cttgcggctc cgtcatcggc aaaattgtgc ccatatgagt
      541 acatcaggca tccattgttt tcgtatcgat agatcagcgg tttgcctgag aatggatcga
      601 tcggtatcga agccgcatct ggaaggatca tctccagacg tggcggaaac gttccggtcg
      661 tgatctgtgc ttttttcaga agggcggcga gatggacaag atccaggcgt acggcaaccg
      721 ttaaggaatg tctgatcaat tcagaatagt ttggtacggc gatgctgaga accacatctc
      781 catacagatc ggttatggcc gacacctttg tccaccgcgt ggtgaataga atgactttta
      841 ctattctcca gatgagttga ttcttgtcgt cgagccaatc aaaactgttt ggctgtgtgc
      901 tccagacagt gagagtctca agctttcgtt tcaagtccgg gtccgagaga attttgctgt
      961 gagaagcttt cagttgaagg aatcggcgag cgagttgatt gaaatcaacg tattgttgca
     1021 cggcgtgatt tttgtcatac gcctggttat ataacaagac aaattgccag agatgctctg
     1081 tctccaatgc gttgttcata ctcgggatcg gcctgagtac cgagagcgag tctgccagtt
     1141 ggtcacacag tttcggggag agagacgcaa tggtgatcca tgcttcacaa gtcttcagtg
     1201 tctcgtcgag cgtcaggagt ccgagaagct tatccacgag aaagtcagtt tcggtgacgt
     1261 gacgtgaaag gcgtaaggca atctgcaggt cgctcagact cccgtctgtg tctccagtcc
     1321 ccagacgtcg catagatcga gccagcaggc cgcggtgtgc ctgaacatag tggcttcgac
     1381 caaaaagctt gcctgccaga agatcgtcac cagaatctcc ttgaagaaat ggccaccaga
     1441 aacgtttgcg ttggcttgcg tcggcgagga gtgcgaacga ttgctggttt gcgttgatcc
     1501 aggagtcgac atccgggtat tcgctggcct tccagggttt acagccgcat tctttcagtc
     1561 tcactttccc gatatcgacc ggtggctgag gaaactctgc atcgattggc ctgggtgtca
     1621 acgacgctgc caggaaagct gccgatgctg catcgcgagt gccagagaca acctcgggag
     1681 ttgaaactgc ggaagatgcc agaaactcat ccaatgggcg ataggacaaa tcggcgggaa
     1741 ggggaaaagg cgtgccaagt aagctggcaa tcgccgcggc ttcggttccg attggaagac
     1801 ttgctgggga atagagacgc agcaggggaa tgacgacatt gtcggcagga gagaccgggc
     1861 cgtaacgcag attcagcgcc tgtccataat ccacgcgacc agatggtgtc agcggactcg
     1921 tgaagtaagt ggtttcagga gaaatctgaa aagcctttgg cggctcgatt gtgagaaaaa
     1981 tcagatagta cggaatgccg agaatgacga tccagagttt ccaccggaac cgaagaaaag
     2041 aaaacacgat ctgcacctct cacgaatgaa tgctggcgaa acggtatcga gattccgaag
     2101 aaaggtcagc gaagctgggc atacagcgcc aacgcctcgc gccacggcag ggtttcaatt
     2161 ccgtggttct gctgttttct tgaagcgttc atcgagacta caacgaggcg cacatcggga
     2221 aaacgggtat ggaacttttc ggccgtggcg gg
//
LOCUS       DAORHD010000858         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59503_length_2252_cov_2.548020, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000858 DAORHD010000000
VERSION     DAORHD010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59503_length_2252_cov_2.548020"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2252
                     /locus_tag="PKO06_16335"
     CDS             <1..>2252
                     /locus_tag="PKO06_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV71274.1"
                     /translation="KTAKVEKTPELWWYSGKANDPAIDDEVKAIIRERLDLNLLIEAV
                     PGSGKTYNLVQRMISLILARHPANQIAAITFTRKAAEELRSRFREQVESRIGSSSGEE
                     RTLLEQALLAIEDGYIGTIHGFCSRILREYPIEAQLDPSFIELEEGQDVDLFKQAWIE
                     LIGRRADWVAIFSDHGLDPMDSIIVKGLKRFCEFPDADFSVVDKPPAPDPKPLLQAFE
                     ELWKTTVAYDPRPRMSEDEKRENNELMMGWVKYDRTKQSIQSFLGLQTFSPNDLKALW
                     RALRYMSRTVMRTDDKNANEALKESCGAVVKLADAFAAAYAEYVFPAILPLLNGLRDA
                     EREWRNRRGFVSFLDLLSRTVQLLKNQPVIREAVSRRYPVLLVDEYQDTDPFQAQMVF
                     LLTATDSCEKDWMKCVPKPGSLFLVGDPKQSVYRFRRADIQVYRQTAEHLKRSGGQVL
                     TLKHNRRSLSSLCEWVNRAFAPVFQEQQQVGQAPFAEMFPMRASEGKAAVYAYQIPDS
                     IRGNATRKMCEHDAAAFAGIVASMLGRSFPNVNDGRPLQPDDVLILCRKNDLLVLYAK
                     ELEARGIPTNLSGGGKELASTLQKEFEPLYWLLRALAECTNAAVVWAVLVGAFFGVSD
                     EEMWEYVRSGGRLNCAFPPDGDSSVARALCRLAQYRRWMQDEIPGTAFRRIVHDLGYD
                     AHLQISEHGLMKTTFLGDVERLFDNMLFSDAVDTLGVWITNWPVDRKPDEPGRVRIMT
                     IHKAKGLEAP"
BASE COUNT          502 a          586 c          673 g          491 t
ORIGIN      
        1 gaagacggca aaagtcgaga agactccgga actctggtgg tattcaggaa aagccaatga
       61 tccggccatc gatgatgaag tgaaggcgat cattcgcgaa cgactcgacc tcaaccttct
      121 gattgaggcc gtgccggggt ctggcaagac gtataatctg gtgcagagaa tgatctcatt
      181 gattctcgcc cgtcaccccg cgaatcagat cgcggcgatc accttcacgc gcaaggcggc
      241 cgaggaactg cgaagtcgtt ttcgagaaca ggtagagagc cgtataggaa gctcttcggg
      301 cgaggagaga actcttcttg aacaggctct gctggcgatc gaggatggct acatcggtac
      361 gatacacggg ttctgctcac gaattctcag agaatatccg attgaggcac aactcgaccc
      421 ctcgttcatc gaactggagg aaggacagga cgtcgatctg ttcaagcaag cctggataga
      481 actcatcggt cgccgtgccg attgggtcgc gatcttttcc gatcacggcc tggacccaat
      541 ggattcgatc atcgtcaagg gattgaagcg gttctgcgag tttcctgacg cggacttttc
      601 cgttgtcgac aaaccgcctg cacccgaccc gaaaccgctg ctgcaggctt ttgaggagct
      661 ctggaaaacg actgtcgcct atgacccgag gcctcgcatg tctgaagacg aaaaacgtga
      721 aaacaatgaa ctgatgatgg gctgggtgaa atacgaccgg acgaaacaat caatacagtc
      781 atttctcggt ttgcagacat tttccccgaa tgacctgaaa gcgctgtggc gcgctctgcg
      841 ttacatgtcg cgaaccgtca tgcgtaccga tgacaagaat gcgaatgagg ctctgaaaga
      901 gagttgcgga gccgtcgtga aactcgccga tgcctttgcc gcggcttatg cggaatacgt
      961 gttcccagcg attctgccgc tcctgaacgg cttgcgcgac gccgagcggg aatggcgcaa
     1021 tcgccgcgga ttcgtcagct ttctggacct gctctcgcgg accgttcagt tgctgaaaaa
     1081 ccagccggtc attcgcgagg cggtgtcgag acggtatccg gtgttactcg ttgacgagta
     1141 tcaggatacc gacccctttc aggctcagat ggtttttctt ctgacggcca ccgactcgtg
     1201 cgaaaaggat tggatgaagt gcgtgccgaa gccaggttct ctgtttctgg tgggcgatcc
     1261 gaaacagtcg gtgtaccggt ttcgacgagc cgacattcag gtatacaggc agacggccga
     1321 acacttgaaa cgaagcggcg ggcaggttct caccctgaaa cacaaccgca gatcgttgag
     1381 cagtctgtgc gagtgggtga atcgagcctt cgcgccggtg tttcaagaac aacagcaggt
     1441 cggtcaggcc ccgtttgccg agatgtttcc gatgcgggcc tcagaaggga aagccgcggt
     1501 gtatgcgtat caaattccgg attcgatcag aggaaacgcg acgaggaaaa tgtgcgaaca
     1561 cgatgccgcg gctttcgccg ggatcgtcgc ctcgatgctt ggcaggagtt tcccgaacgt
     1621 taacgatggt cgaccgcttc agccggacga tgttctgatt ctctgccgaa agaacgacct
     1681 acttgttcta tatgcgaaag agcttgaagc gcgggggatt ccgaccaacc tttccggggg
     1741 tggaaaagaa ctcgcctcaa ctctgcagaa ggagttcgaa ccgctgtact ggcttctgcg
     1801 ggctcttgcc gagtgcacaa atgccgccgt ggtctgggct gttcttgtcg gggcgttttt
     1861 cggcgtatcc gatgaggaga tgtgggagta cgttcgctcc ggcgggcgtc tgaactgcgc
     1921 gttcccacca gacggagact cttccgtggc gagggctttg tgtcgtctgg cgcaatacag
     1981 gcgctggatg caggatgaga tcccgggtac agcttttcgc cgtatcgtgc atgacctcgg
     2041 ttatgatgct catctgcaga tttccgaaca cggactcatg aagacgacct tcctcggaga
     2101 tgtcgagcgg cttttcgata acatgctgtt cagcgatgcc gtagacacgc ttggagtctg
     2161 gattacgaac tggccggtcg atcgcaagcc tgatgagcct ggtcgcgtga gaatcatgac
     2221 cattcacaag gcaaaggggc tggaagctcc gg
//
LOCUS       DAORHD010000859         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59522_length_2251_cov_3.464936, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000859 DAORHD010000000
VERSION     DAORHD010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59522_length_2251_cov_3.464936"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1730)
                     /locus_tag="PKO06_16340"
     CDS             complement(<1..1730)
                     /locus_tag="PKO06_16340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71275.1"
                     /translation="MPTDDQGLAVSSAARVARAPMPDKIARACLELILQNISKSSLSA
                     IVVSWVLIYFQKDFFSTRALASWAVVTTLVFAVRWYFSVWVLRHDPTLQRQLVWRRCT
                     FALAMVQALCWGYSAWFFFPLEFSLMQAFHLVIITSIGAASVTVLAPLHEVIHGYLLL
                     SQLPLFLRTLLIHDPQHYMMSVMIVLFLFIVWMNATRMHQVIIDSYQLRFTNEDLIEE
                     LREAKQAADAASQAKTTFLANMSHEIRTPMNAVLGFTALLQADDPLPHQRERLEIIQT
                     SAKALLRILDNILDLSKIETAHMRLEQDSFDPEPVICEPVELASALLGDRPVEVVSDV
                     DALPPMLTGDGFRLRQILMNLLGNAVKFTHTGFVQLTVRVTETSSQLFKLLFAISDTG
                     IGIPQEHLQKIFEPFSQSDDSVSRRFGGTGLGLAISAKLVSLMGGTLRVTSELNQGST
                     FSFELPFRAVSTPAPEVVQPGFAHVLIIDDVSRTSDVIATQMRKNGSTVVQVRSLQEA
                     VLTMANTAFDLICVDLTRPGNDPATCSELLRRQARSETTPRFLGITAINRVGVRQSRD
                     LGFDAFCRKPL"
     gene            1856..>2251
                     /locus_tag="PKO06_16345"
     CDS             1856..>2251
                     /locus_tag="PKO06_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71276.1"
                     /translation="MTSFFSPAPHRMPVRSELVEPLYLRLRWQVLASTFFGYAIFYIV
                     RNNLPPVAKEMGAALEYDKSMMGTILAGTALSYGVGKLVMGAFSDRSNPRRFMPFALV
                     LTALLNFWFGLSRGYSMHLFLWCLNGFVQG"
BASE COUNT          487 a          577 c          681 g          506 t
ORIGIN      
        1 aggggtttgc ggcagaaggc gtcgaaacca agatcgcgac tttgtctgac accgacgcga
       61 ttgatggcgg tgatgccgag gaatcgtggt gtcgtctcgg atcgcgcctg tcgccgaagc
      121 agttcggagc aggtcgccgg gtcgttgcct ggtcgcgtca aatcgacgca gatgagatcg
      181 aaagccgtat tagccatggt caatacagct tcttgcagag aacgaacctg gacgactgta
      241 cttccgttct tgcgcatctg ggtggcgatc acgtctgagg tgcgcgacac gtcgtcgatg
      301 atcaggacgt gggcgaaacc tggctgaacg acttccgggg ccggtgtcga aacggctcgg
      361 aaaggcagtt caaaagagaa ggttgagcct tggttgagct ctgaagtgac gcgcagtgtg
      421 ccgcccatca gggagaccag tttggcgctg atggccaatc cgagcccggt accgccgaat
      481 cgtcgtgata ccgaatcgtc tgactgtgaa aagggctcga agatcttctg caggtgttcc
      541 tgcggaatgc cgattcctgt atccgaaatg gcaaacagca gcttgaacag ctgcgaagaa
      601 gtttcggtga ctcggacagt cagttggacg aatccggtgt gcgtgaattt tacggcgttg
      661 ccgagcaagt tcatcaggat ctgccgcagt ctgaatccgt cgccggtcag catgggcggc
      721 agggcgtcca cgtcgctgac aacttcgacc ggtcggtcgc caagcaacgc agaagctagt
      781 tccactggtt cacagatgac cggctcgggg tcaaacgaat cctgctccag acgcatgtgc
      841 gcggtttcga ttttcgacag atcgagaatg ttgtcgagaa tgcgaagcag ggctttcgcg
      901 cttgtctgaa tgatctcgag tcgctcgcgc tgatgtggga gcggatcgtc ggcctgcagc
      961 agagccgtga acccgagaac ggcgttcatg ggggttctga tctcgtgact catgttggca
     1021 agaaaggtgg tcttggcttg actggccgcg tcggcggcct gcttggcttc gcgcaactct
     1081 tcgatcaggt cttcgttggt gaatcgcagt tgatacgagt cgatgatgac ctgatgcatc
     1141 cgtgtggcgt tcatccagac gatgaacaga aacaacacga tcatgacgct catcatgtaa
     1201 tgctgcgggt cgtggatcag cagtgttcgc aggaacagcg gcagctgact gagcagcaga
     1261 tacccgtgaa tcacctcgtg aaggggagcc agcacggtga ccgaagcggc accgattgat
     1321 gtaatgatga ccaggtgaaa agcctgcatc agagaaaact cgagcggaaa gaaaaaccac
     1381 gcggaataac cccagcagag tgcctgaacc atggcgagag caaaggtgca gcggcgccag
     1441 acaagctgtc gctgcagggt tgggtcgtgt ctcaggaccc agacggaaaa gtaccagcga
     1501 acggcgaaga ccagagtggt gaccacggcc caggaggcca aggctctcgt ggaaaagaag
     1561 tctttctgaa aatagatcag gacccatgaa acgacgatgg cgctgagcga acttttgctg
     1621 atgttctgaa gaatcagctc gagacaggcg cgcgcgattt tgtccggcat gggagcgcga
     1681 gcaacgcggg ctgccgaaga tactgccaga ccctgatcat cggtcggcat ctggatcttt
     1741 cgctctcgag agcattgagg tgtcgtccaa catagacttc aaacccctca tagtatcact
     1801 attttcctct ttgatctgat gtttcgattc cagtatgatg ccctggatac tgcttatgac
     1861 atccttcttt tctccagccc cgcatcgtat gcctgtccgg agcgaactgg tcgagcctct
     1921 ctaccttcgt ctgcgctggc aggttctggc ttcgactttc tttggctacg cgatctttta
     1981 catcgtcaga aacaatcttc cccccgtggc caaggaaatg ggggcagctc tcgaatacga
     2041 caagtcgatg atgggcacga ttctggctgg aacggctctc tcatatgggg tcggcaagct
     2101 ggtgatgggg gctttttccg atcgctcgaa tccacgccgg ttcatgccgt ttgctctggt
     2161 gctgacggcg ttactcaact tctggtttgg cctttcccgt ggctactcca tgcatctgtt
     2221 tttatggtgt ctgaacgggt ttgttcaggg t
//
LOCUS       DAORHD010000860         2249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59589_length_2249_cov_3.278943, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000860 DAORHD010000000
VERSION     DAORHD010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59589_length_2249_cov_3.278943"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2249)
                     /locus_tag="PKO06_16350"
     CDS             complement(<1..>2249)
                     /locus_tag="PKO06_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HNV71277.1"
                     /translation="NRLNFGGTNCVVDAACASSLSALNLAVLELASRRCDLVVTGGVD
                     TFNDIFMYMCFSKTPALSPTGHARPFSADCDGTTLGEGIGMVVLKRLADAQAAGDRVY
                     AIIKGVGTSSDGKGKAIYAPSSQGQMKALRRAYEQAGVTPQQIGLLEAHGTGTKVGDQ
                     IEISALREVYPSPENQRPYCGIGSVKSMIGHTKAAAGVAGLIRVSLALYHKVMPPTLK
                     ISKPAPGVMDNDSPFFLLGERHPWFINGYQPRRAAVSSFGFRGSNYHAVLEEGNSRKL
                     TRDWDSTHEMFAFSGDTPATVREMLGAISGLKNADEIRTQAALSRRRFQATQACRLAL
                     LVELGKTDLAKLVQSVTAKLGEAGVKHFSLTEGAWYGTGPVNGKMAWLFPGQGAQYPH
                     MGRDLAILSPEMFTSIAEASALLGEIEPQNRTLTDCLYPPHWFSPEAAKADEDRLKTT
                     QVAQPAIGAVSLGMAALLQSWGLRPEGAVGHSFGELTALCVAGAFNAESLIRMSRKRG
                     ELMGQGTGDRGGMLAVSAPAAEVAQVLKQAGLDLTLANHNAPDQIVISGRKGLLEKAR
                     ELCQARKWRSTLLQVAAAFHSPLVADAAVPFREALENVSWNAVQIPVYANTTAEPYPT
                     DQQQAKDLLGSQLARPVRFVDSIERMYADGYRVFVEVGPGGRLSGLARSILGDRPVTI
                     LTLDASSGRKPGTGDLGRVLAPLAALGYPVQINGWEDGELWLEKCQNEKHSGLSVKLN
                     GANYRTPRP"
BASE COUNT          429 a          729 c          659 g          432 t
ORIGIN      
        1 ccgggcgtgg ggttcgatag ttcgcgccat tcaattttac ggagagtccg gaatgctttt
       61 cgttctggca cttttcaagc cagagttctc catcttccca gccgttgatc tgaaccggat
      121 acccgagagc tgccagggga gcgagaaccc ggccgaggtc accagtaccg ggcttgcgtc
      181 cactcgaagc atcgagggtg agtatggtga ccggacgatc accgaggatg gaccgagcaa
      241 gccccgacaa tcggccaccc gggccgactt cgacaaacac gcgatacccg tcggcataca
      301 tgcgctcgat cgagtcgacg aaccgcaccg gccgtgccaa ctgcgaacca agcagatctt
      361 tggcctgctg ctgatcggtc ggatacggct cggccgtggt attggcatag accggaatct
      421 gtacggcatt ccacgagaca ttttcgagag cttcacgaaa cggcacggct gcatcggcca
      481 ccagcgggct gtgaaatgcc gcggccacct gcagcagcgt cgagcgccac tttctcgcct
      541 gacagagttc tcgcgccttc tcgagcagac ctttgcgccc cgagatgacg atctgatctg
      601 gcgcgttgtg attcgccagc gtgaggtcga gcccagcctg tttcagaacc tgcgcaactt
      661 cggccgccgg cgcggaaacg gccagcatgc cgccgcggtc tccggtcccc tgccccatca
      721 attctccgcg tttgcgcgac atgcggatca gcgactcggc attgaaagca ccggcgacgc
      781 acagggcggt cagttcaccg aacgagtgcc cgacggcccc ttccgggcgc agcccccaac
      841 tttgcagcag agcggccatg cccagtgaca cggctccgat ggccggttgc gcgacctgag
      901 tggtcttcag acggtcttca tcagctttcg ccgcttcagg cgagaaccag tggggcggat
      961 acaggcagtc cgtcagcgtg cggttctgcg gctcgatttc gccgagaagc gcagacgcct
     1021 cagcgatact ggtaaacatc tcaggcgaca gaatggccag atcgcgcccc atgtgcgggt
     1081 actgcgcccc ctgccccggg aacagccacg ccatcttgcc gttgaccggc ccggttccgt
     1141 accaggcccc ctcggtcagc gaaaagtgtt tgacgccagc ttcgccgagt ttggccgtga
     1201 cactttgcac cagttttgcc aggtcggtct tgccgagttc aaccagcagt gccagccggc
     1261 acgcctgtgt cgcctgaaaa cgtcgtcggg aaagagcggc ctgggttctg atctcgtcgg
     1321 catttttcaa cccactgatc gcgccgagca tctctcgaac cgttgcgggc gtatcacccg
     1381 aaaacgcaaa catctcgtgc gtggaatccc aatcgcgggt cagtttgcgc gaattccctt
     1441 cttcgagaac cgcgtgataa ttgctccccc tgaacccgaa agacgaaacc gccgcgcgac
     1501 gcggctggta cccgttgata aaccacggat gtcgctctcc gagcaggaag aaggggctgt
     1561 cgttgtccat gacaccgggg gccggcttcg agatcttcag cgtcggcggc ataactttat
     1621 ggtagagtgc cagcgacact ctgatcaaac ccgcaacccc agcggctgcc ttggtatgcc
     1681 caatcatcga ttttacggaa ccgatgccgc aatacgggcg ctggttttcc ggtgaagggt
     1741 agacttcgcg cagagccgag atctcgatct gatcgccaac tttcgtgccc gtgccgtgcg
     1801 cttccagcag gccgatctgc tgcggtgtca ccccggcctg ctcatacgcg cggcgtaacg
     1861 ctttcatctg cccctgcgaa ctgggagcat agatcgcttt gcctttaccg tcgctcgacg
     1921 tgccgacgcc cttgatgatg gcatacacgc gatccccggc cgcctgtgca tccgccaggc
     1981 gcttcagcac caccatgccg attccttctc ccaacgtcgt tccatcgcag tcggcgctga
     2041 acgggcgggc atggccggtt ggcgaaagcg cgggcgtctt gctgaaacac atatacatga
     2101 agatgtcgtt gaaggtgtcg acgccgccgg tcaccaccag gtcgcagcgt cgcgaagcca
     2161 gttcgagcac ggcaagattc agcgcgctca gcgagctggc acaggccgca tcgaccacgc
     2221 agttggtgcc accaaaattc aggcggttg
//
LOCUS       DAORHD010000861         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59613_length_2248_cov_4.113087, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000861 DAORHD010000000
VERSION     DAORHD010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59613_length_2248_cov_4.113087"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..907)
                     /locus_tag="PKO06_16355"
     CDS             complement(20..907)
                     /locus_tag="PKO06_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV71278.1"
                     /translation="MASGLTIFLAWTFALSGLPIGAAEGNPLSMLILESKNDEPLKVV
                     TGETSRLDQPESAASTFKIWLTALALEEKILTRSSRYDCCEPHLYHGKKARVGLRQAL
                     ILSSNDFFREVASRLGLKKLNQGLASLHLTPKPQTRSVRSIAAAIHGDRFRVTPRQQH
                     EAMRALVLGSLPVSRETRQTLREALEWPVPENAHWRVYGKTGCYGGAVWFVGWGESRP
                     TLVPSGAIASQPRPEVKIVTVFAPGNLTQRPEVMRQFFRRFGIDWSDTLLQGLGATGK
                     LPDSTTNTGSEPAATGTKP"
     gene            complement(913..>2248)
                     /locus_tag="PKO06_16360"
     CDS             complement(913..>2248)
                     /locus_tag="PKO06_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71279.1"
                     /translation="IGSGIPDFYPQSWSESLFRWIMAQTTDSTILVLSTVDETGLVPR
                     YLEQLGHDRVATLTISERYIADDPAVAARLDAAGGIIVLDRDPGQILNLWKGTRTEAA
                     MQRAFSGGRVLVGMGGGAQALLGLIPDAGTRSTTPGQVVRRPVAATMTCELGLTGSVG
                     EIFVEPFFTRRARLPLLMNVMARRRQQNNAAVILGLGIDEQTGVIWSADDHLTVHGAG
                     AVSFLRFTSESVIRLDPRLPPVITALALDQLTEGFMINLSTAEVVLVPETATTGFSSA
                     SLVISGDQVLEGARIGHPQPGVWEVTQLDRHSYALQRGLLDEVFGEGRFDGIRLITGA
                     FINADHDENRLGGLTWTLANHPGSWGILMDRGSKADLLASGSLVPLPMTDQPGVLVID
                     ATRLGHRDYSRFKTHLDGRGPRQSVALTNLRLHLIATRCDVNLRSGVVTVRP"
BASE COUNT          486 a          715 c          627 g          420 t
ORIGIN      
        1 cccacgaaca acggaatggt cacggttttg taccagtcgc cgcaggctca cttccagtat
       61 tcgtcgtcga atcaggcagc ttacctgttg cgccgagacc ctgcagaagg gtgtcactcc
      121 agtcaatgcc gaagcggcga aagaactgtc gcatgacctc ggggcgttgc gtcagattcc
      181 ccggggcaaa cacggtcacg attttaactt cgggacgcgg ctgcgacgca atcgccccgg
      241 acggcaccag cgtcgggcga gattcacccc acccgacaaa ccacaccgct ccgccatagc
      301 aaccggtctt tccgtatacc cgccagtggg cgttctcagg aaccggccat tcgagcgcct
      361 cgcgcagggt ctgacgtgtc tcgcgtgaca ccggcagaga accgagaacg agggcgcgca
      421 tcgcttcatg ctgttgccgc ggagtcacac gaaagcgatc tccatgaatg gcggcggcta
      481 tcgaacgaac agaacgtgtc tgcggcttcg gtgtcagatg cagcgacgcc agcccctggt
      541 tgagcttttt cagacccagg cgactcgcca cttcacgaaa gaagtcattg ctcgagagaa
      601 tcagcgcttg acgcaatccg acccgggcct tttttccgtg atagagatgc ggttcacaac
      661 aatcgtaccg gctcgatcgt gtcagaatct tctcctcaag cgctaaagcc gtcaaccaga
      721 tcttgaaagt cgaagcagcg ctctccggtt gatccagccg tgaggtctcg ccagtcacca
      781 ccttcagagg ttcatcattc ttcgactcaa gaatgagcat cgacaaaggg ttgccttcag
      841 ccgctccaat cggcagaccg ctcaaagcga aggtccaggc gagaaaaata gtcagcccgc
      901 ttgccatgct cttcatggtc gaacggtaac gacaccgctg cgcagattca cgtcacagcg
      961 agtcgcgatc aggtgcaatc gcagattggt caaagccacc gactgtcgcg ggcctcttcc
     1021 gtcgaggtgt gtcttgaaac gcgaatagtc acgatgtccc aggcgcgtgg catcaatcac
     1081 cagcactcca ggctgatcgg tcatcggcag tggcaccaga cttcccgatg ccagaagatc
     1141 cgccttgctg cctcgatcca tgagaattcc ccaagacccc gggtgattcg ccaaggtcca
     1201 ggttaggcct cccaggcggt tttcgtcatg atccgcattg atgaatgcgc ccgtgatcag
     1261 gcgaatcccg tcaaaacgac cttcgccaaa aacttcatca agcaagccac gttgcaacgc
     1321 gtaggagtgg cggtccagct gcgtgacctc ccagaccccc ggttgcggat ggccgatacg
     1381 ggctccctcg agcacctggt caccgctgat caccagagag gccgacgaga agccggtcgt
     1441 cgcggtctca ggcaccagaa ccacttcggc ggtcgagaga ttgatcatga aaccttcggt
     1501 caactgatca agcgccagtg cagtgatcac cggtggcagc cgtgggtcga gtcgaatcac
     1561 tgattcggag gtaaatcgaa gaaagctgac ggcgcctgcc ccgtgaaccg tcaggtggtc
     1621 gtcggcagac cagatcaccc ctgtctgttc atcgattccc aggccgagga tcaccgcagc
     1681 attgttttgc tgacgtcggc gcgccatcac gttcatcagt aacggaagac gcgcgcggcg
     1741 ggtaaagaag ggttcgacga agatctcgcc cacgcttccc gtcagtccaa gttcacaggt
     1801 catcgtggcg gcaaccggcc tgcgcaccac ctgtccagga gtcgtcgatc gggtgccggc
     1861 atcgggaatg agccccagca aagcctgtgc cccgcccccc atgccgacca gcacccgccc
     1921 tcccgaaaac gctcgctgca tcgctgcttc tgtgcgagtg cccttccaaa ggttgagaat
     1981 ctggccgggg tcgcgatcga gcacgataat gccgccggcg gcatcgaggc gcgctgctac
     2041 cgccggatca tccgcaatgt agcgttccga aatcgtcagg gtcgcaaccc ggtcatgtcc
     2101 cagttgctcg agatatcgcg gcaccagccc cgtttcatcg accgtcgaca gcacgagaat
     2161 cgtcgagtcc gtggtttgag ccatgatcca gcgaaacaga ctctcgctcc acgactgcgg
     2221 atagaagtcc gggatgccgc ttccaatc
//
LOCUS       DAORHD010000862         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59635_length_2248_cov_2.868673, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000862 DAORHD010000000
VERSION     DAORHD010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59635_length_2248_cov_2.868673"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PKO06_16365"
     CDS             complement(<1..294)
                     /locus_tag="PKO06_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007094786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV71280.1"
                     /translation="MIEFLSCRKINDRFREEFTELFQRFLDRGQYILGEQVVLFEEAF
                     AAFCGTRHCVGVGNGLDALELILHAYQELGRLSRGDEILVPANTYIATILSIVR"
     gene            complement(302..805)
                     /locus_tag="PKO06_16370"
     CDS             complement(302..805)
                     /locus_tag="PKO06_16370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV71281.1"
                     /translation="MSHHLTYMIHPAADVQCSTIGDGTRVWQFVIILPGARLGSHCNI
                     NAHCLIENDVQIGDHVTVKCGVYLWDGITLEDHVFVGPNVTFTNDRFPRSQQKPPSFE
                     RTIVRRGASIGAGAVILCGLTIGEQAMIGAGSVVTKDVPPGELWYGNPARPRGRAPRA
                     DLLKRER"
     gene            complement(802..1236)
                     /locus_tag="PKO06_16375"
     CDS             complement(802..1236)
                     /locus_tag="PKO06_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006987736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNV71282.1"
                     /translation="MKTFANSRVFDCPVLALPVVHNPAGNISYANSRENIPFEIKRVY
                     YLYDVPAGETRGGHAHRQLYELLFAAQGSYDVVLDDGNCKSIIHLNRPHLGLLIVPGI
                     WRELINFSSGAISLVLASEVYDESDYIREYDQFIREKKPEQP"
     gene            complement(1233..1631)
                     /locus_tag="PKO06_16380"
     CDS             complement(1233..1631)
                     /locus_tag="PKO06_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HNV71283.1"
                     /translation="MNYLGIYSLPKVEDQRGNLTFLQSPMNVPFAIGRVYWIYGVPGG
                     QTRGSHAFRSAEEVMIALSGSFDVRLDNGRQEMVFHLNRSYMGVYIPRLHWRTLENFS
                     TNSLCLVLSSTAYDATDYIRSYDEFRSLTE"
     gene            complement(1631..>2248)
                     /locus_tag="PKO06_16385"
     CDS             complement(1631..>2248)
                     /locus_tag="PKO06_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71284.1"
                     /translation="FVSHNIAAVKALCDKGLVLQRGRIAYWGDILPAIQFYQNSGTHR
                     TGIDYRARPTEAPGNRNIRLLCFTVRPKAGVLLNITSGVSFSLEFAYLEEKCSLDVTF
                     ELRNAEEVPVFHVACDIKSCQEKGAGVYRISGIIPPYFLNAGIFSFSLIFGRSRKDVL
                     YLCDDALSFEIQEEVIDAWPSSRRGLTFPRLEFHAEYLGSTVKEE"
BASE COUNT          598 a          614 c          533 g          503 t
ORIGIN      
        1 gcgaacaatg gacagaatgg tggcaatata ggtattggca ggtaccagga tctcatcgcc
       61 gcggctcaat cgacccagtt cctggtatgc atgcagaatg agttccagcg catccagacc
      121 atttccgacc ccgacacagt ggcgggttcc gcaaaacgcc gcaaacgctt cttcaaagag
      181 tacgacctgt tctccgagaa tatactgtcc acgatcaaga aaccgttgaa agagttcagt
      241 aaattcctct cgaaaccggt cgttgatttt gcgacaactg agaaattcga tcatgacagg
      301 atcaccgctc ccgttttaaa agatcggcgc gtggtgctct tccccttggt cgagccgggt
      361 tgccatacca cagttcaccc ggcggcacat ctttcgtgac cacgctgccg gcaccgatca
      421 tcgcctgttc accgatcgtc aatccgcaca gaatcactgc cccggctcca atcgacgctc
      481 ctcgtcgaac gatcgttcgc tcgaaactcg gaggtttctg ctgtgaccgg gggaatcgat
      541 cattcgtgaa ggtcacgttg ggaccaacaa agacgtggtc ttcaagcgtg attccatccc
      601 acagatacac cccacacttc acagtcacgt gatcgccaat ctgaacatcg ttttcaatca
      661 gacagtgcgc attgatattg cagtgactgc ccaatctcgc acccggcaga atgatgacaa
      721 actgccagac gcgcgttccg tcaccgattg tcgagcactg aacatcggcg gccggatgaa
      781 tcatataagt cagatgatga ctcatggctg ttccggcttt ttttcccgga taaactgatc
      841 atattctctg atgtagtctg attcatcgta tacttctgaa gccaggacaa gagaaatcgc
      901 tcccgaactg aaattaatca gttcgcgcca gattccaggg acaatcagaa gcccgaggtg
      961 tggacgattc aagtgaatga tcgacttgca attaccgtca tcgagaacga catcgtaact
     1021 cccctgggcc gcaaacagca gttcatacaa ttgtcgatgt gcatgtcctc ctcgagtctc
     1081 tccggccggt acatcgtaca ggtagtatac acgtttaatc tcgaacggaa tgttttctct
     1141 actgttcgcg taggagatgt ttcctgccgg gttatgcacg accggcaatg cgagtaccgg
     1201 gcagtcaaag acccgactgt tcgcaaaggt tttcattcag ttagactcct gaattcatca
     1261 taactccgaa tatagtcagt cgcatcatac gccgtcgatg aaagcaccag acacagggag
     1321 ttcgtcgaaa agttttcgag ggttcgccag tgaagtcggg gaatatagac gcccatgtac
     1381 gaacggttca gatgaaaaac catctcttgt cgaccgttgt cgagacgcac atcaaatgac
     1441 ccggacagcg caatcatcac ctcttcagcc gaacgaaacg catgcgagcc ccgcgtctgg
     1501 ccaccgggaa cgccgtagat ccagtatact cgaccgatcg caaaagggac attcatcggc
     1561 gactgcagaa acgtgagatt gccgcgctga tcctccactt tcggtaacga atagatgcca
     1621 aggtagttca ttactcctcc ttcaccgttg agccaagata ctcggcgtga aactccaatc
     1681 gtggaaacgt caaccctcgc cgcgacgacg gccacgcatc aatgacttct tcctgaatct
     1741 caaatgaaag cgcatcatcg cagagataca gaacatcttt ccgagatctc ccaaaaatca
     1801 atgagaagct gaaaatgccg gcgttcaaaa aatacggggg tatgattccg ctgatgcgat
     1861 agacaccagc ccccttttcc tgacaagatt tgatgtcaca ggccacatga aaaacaggaa
     1921 cttcttcagc atttctcaat tcaaaagtga catccagact gcatttctct tccagatagg
     1981 caaactcgag agagaacgag actcccgagg tgatattgag cagaacacct gccttcgggc
     2041 gaacagtaaa acaaagaaga cgaatgtttc gattgccggg agcttcagtt ggtcgagcgc
     2101 gataatcgat gcctgttcga tgagttccgg agttctgata aaactgaatg gccggcaaaa
     2161 tgtcgcccca gtaggcgatc cgcccgcgtt gaagaaccaa acccttgtcg cagagggcct
     2221 tgactgccgc gatgttgtga ctgacgaa
//
LOCUS       DAORHD010000863         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59756_length_2245_cov_4.260731, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000863 DAORHD010000000
VERSION     DAORHD010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59756_length_2245_cov_4.260731"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="PKO06_16390"
     CDS             <1..1235
                     /locus_tag="PKO06_16390"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNV71285.1"
                     /translation="TQYFLSPGGRNRIRDRNLRAHSRRLLIQYSEHLGRSDKDAFETI
                     GACIQELRGLFLQGSTGYLRGIDLQLHEMDDYIQESKTKRRGGMGYDNRFPILSEKLG
                     GLQREFYLFTGGVGMGKSTFVTQIAWDLVEQNPDLTVVYFSLDLARMDVLAKMVGQAG
                     EVPIDYVRNPYVTSSDFDQRRREGLEKVSNMRERLFIVDESNGRIFIDDIRKLVKRTR
                     LERGGEVAVVIDPVFKIHLKQDRISFTEKCNFLVSELKSIAASENVCLIATAGLPKAI
                     SNRRPVREDLEEIMGLLYDPYVVCFLYCDFLNDFETPFLEWELGKENFMIPITEISIA
                     KNKMGSINNRIFYRYYEMFAKFKECAPAEVENYSAMVENLQKFKDDKTLKNKIRAATG
                     GGAKNGGGNTITIREDEF"
     gene            1235..>2245
                     /locus_tag="PKO06_16395"
     CDS             1235..>2245
                     /locus_tag="PKO06_16395"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71286.1"
                     /translation="MNESAVSSFQQGVEYQERGLFDQAIEAYRQALESEPENMDVLVN
                     LGAAYLQKGLAEKSAQVLQKALNLQPEHPLALFNLGKAYLYREDAEKALGVFQRAQQV
                     LPEDPDVKKSIAQCYLLQNKRYEAVELLWELSRAWPGEGSILLLLGRGLLELERFEEA
                     LDVFRKAVTAVPDSSEALNGIIQAQLRLGHTDKAMTTLKRALMVDPRHAPYHLLMVDL
                     LIESDRLEEAISHLKRALSAEPNQPQLRRKMEELTRRLPILKKKAAGQHIAVPVSRYE
                     TEVYDVLDKLYDGKISVDQAVESLERLRDQDPKDTFILEQLAGVFFKARIYPKAMVAY
                     EEL"
BASE COUNT          522 a          612 c          645 g          466 t
ORIGIN      
        1 tcacccagta ttttctctca cccggcgggc gaaatcgtat tcgcgatcgc aatctgcggg
       61 ctcattcccg tcggctgttg atccagtatt ccgaacacct ggggcgctcg gacaaagatg
      121 ccttcgagac catcggcgct tgtattcagg aactgcgcgg tctgtttctg cagggcagta
      181 ccggttatct gcgcggcatc gatctgcagc tgcatgaaat ggacgactac attcaggaaa
      241 gcaagaccaa gcgacgcggg ggcatgggtt acgacaatcg ttttcccatc ctctcggaaa
      301 agctgggcgg gctgcagcgc gagttttatc tgttcacggg cggcgtcggc atgggaaaat
      361 cgactttcgt gacgcagatc gcctgggatc tcgtcgaaca gaaccccgat ctgaccgtcg
      421 tctatttcag cctcgatctc gcgcgcatgg atgtcctggc gaagatggtc gggcaggccg
      481 gcgaggtgcc gatcgactac gttcgcaatc cctacgtcac cagttccgac tttgatcagc
      541 ggcgccgcga agggctcgag aaagtctcga acatgcgcga gcggctgttc atcgtcgatg
      601 aatcgaacgg gcgaatcttc atcgatgaca ttcgcaagct cgtcaagcgc acgcgtctcg
      661 agcgcggtgg tgaagtcgcg gtcgtcatcg atccggtctt caagattcac ttgaaacagg
      721 atcgcatcag cttcaccgaa aagtgtaact ttctggtgtc agaactgaaa agtattgcgg
      781 cgtcagaaaa cgtctgtctg atcgccacgg ccggattgcc gaaggccatc agcaatcgtc
      841 ggccggttcg cgaagacctc gaagaaatca tgggccttct ctacgatccc tacgtggtct
      901 gttttctcta ctgcgacttc ttgaacgatt tcgagacgcc tttccttgag tgggaactcg
      961 gcaaggagaa tttcatgatt ccgatcaccg agattagcat tgcgaaaaac aagatgggaa
     1021 gcatcaacaa ccgtatcttc taccggtact atgagatgtt tgccaagttc aaggagtgcg
     1081 cgcctgccga ggtcgaaaac tactcggcca tggtcgagaa cctgcagaag ttcaaagacg
     1141 acaagaccct gaaaaacaag attcgcgctg ccaccggtgg cggagccaag aacggcggcg
     1201 gtaacaccat tacgatacgc gaggacgagt tctgatgaac gaaagcgcag tcagctcctt
     1261 tcaacagggc gtcgagtatc aggaacgtgg attgtttgac caggcgatcg aagcctatcg
     1321 tcaggcactc gagagcgagc cggaaaacat ggatgtgctg gtgaacctcg gggcagctta
     1381 tctgcagaag gggctggccg aaaaatcggc tcaggtgttg cagaaagccc tgaatctgca
     1441 gccagaacat ccgcttgcgc tcttcaatct cggaaaagcc tacttgtacc gtgaagatgc
     1501 cgagaaagcg cttggcgtgt ttcagcgcgc tcagcaggtg ctgccggaag accccgacgt
     1561 aaaaaaatcg atcgcgcaat gctatctgct gcagaacaaa cggtatgaag ccgtcgaact
     1621 gttgtgggaa ctttcccgcg cctggcccgg agaaggctcg attctgctgc tgctcggtcg
     1681 cggtctgctc gaactcgaac ggttcgaaga agcgcttgat gtgtttcgca aggccgtgac
     1741 ggcggttccc gattcgtccg aggcgctgaa cggcatcatt caggcacagc tgcgtctggg
     1801 gcataccgac aaggccatga cgacactgaa acgggccctg atggtcgacc cccgtcatgc
     1861 gccgtaccac ctgctcatgg tcgatctgct catcgagagc gatcgactcg aagaggcgat
     1921 cagtcatctc aaacgagcgc tttcggctga gccgaatcag ccgcagctgc gccgcaagat
     1981 ggaagaactg acccggcgac tgccgattct gaaaaagaaa gccgcggggc agcatattgc
     2041 cgtaccggtc agccgttatg aaacggaagt ctacgacgtg ctcgacaagc tctatgatgg
     2101 aaagatctcg gttgatcagg ctgttgagag cctcgaacgc ttgcgcgacc aggatcccaa
     2161 ggacacgttc attctcgaac agctggcggg cgtgttcttc aaggcgcgta tctaccccaa
     2221 ggcgatggtt gcctacgaag aactc
//
LOCUS       DAORHD010000864         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59890_length_2242_cov_3.132602, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000864 DAORHD010000000
VERSION     DAORHD010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59890_length_2242_cov_3.132602"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKO06_16400"
     CDS             complement(<1..749)
                     /locus_tag="PKO06_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71287.1"
                     /translation="MVRSFAGRALLAALLVVVSFAFLGCGGGGGGGGDTGNPLVAIPA
                     TNNLQAPVNQPLTQVQQALGVMQPLANQALAGRFTNGLVGAVRANAVRASVAGGEPTI
                     VPVVGNQALNIKGLMNKFMNQLPSAAVKSVPGVGSVDASFKVEFTATTLDVTFYDDDG
                     TTVVETVKATGVTVTGDADRISKVVMGEGTTIVRDDKVTGDKATIVWKGGMTASVEYK
                     QVEGVWTETAIVTPAADGSAFIFEVTYVDKDG"
     gene            complement(1311..>2242)
                     /locus_tag="PKO06_16405"
     CDS             complement(1311..>2242)
                     /locus_tag="PKO06_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNV71288.1"
                     /translation="GLVACCSAQEYWDDKIKKHELTRADKEEDRLKHVDVTDANTGPV
                     FLTYPAKPEIDALIAEVTKGTPEYDFVTSDGIQHVLWVVKDQNWHKRCIELFGTLPAL
                     YVADGHHRSAAAARIAKVRREKNPNHTGNEEYNFFLAVLFPHNQLYIMDYNRAVKDLN
                     GLTPEQFLKKVEEKFTVEKTGEKKPTTATTFGMYLKGQWYKLTAKAGSYKADDPIESL
                     DVSILQNNLLAPILGIGDPRKDKRIDFIGGIRGMNELERIVNEGKFAVSFSMFATSIE
                     QLMKIADAGKIMPPKSTWFEPKLRCGVVTHTLK"
BASE COUNT          503 a          633 c          579 g          527 t
ORIGIN      
        1 ccatctttat ccacgtaggt cacctcaaag ataaaggcac ttccatcggc tgccggagta
       61 acgatcgcag tctccgtcca gacaccttca acctgtttgt actcgacaga ggcagtcatt
      121 ccacctttcc acacgatcgt cgctttatca cctgtgacct tatcgtctcg gacaatggtc
      181 gtcccctcac ccatgacaac cttcgagatt ctgtcagcgt cacccgtgac ggtgacacca
      241 gttgccttca cggtctctac aacggtcgtg ccgtcatcat catagaaggt tacatcgaga
      301 gtcgttgcag tgaattcaac tttgaaactg gcatcaactg aaccaacgcc tggcaccgat
      361 ttaacggcgg ccgatggcaa ctgattcata aacttgttca tcaaaccctt gatgttcaaa
      421 gcctgattac caacaaccgg aacaattgtt ggctctccac ctgcgacaga cgcgcgcacg
      481 gcatttgccc gcacagctcc aaccaaacca ttggtgaaac gacccgccaa cgcctgatta
      541 gccaatggct gcattacgcc gagcgcttgc tgaacctgtg tcagaggttg gttgacagga
      601 gcctgtagat tgtttgtagc gggtattgct actagagggt taccggtatc gccgccaccg
      661 cctccgccgc ctccacagcc gaggaacgca aaagaaacaa cgaccaacag ggcggcaaga
      721 agcgcccgac cagcaaaaga acgaaccatc tccaactcct ccatacagtt tatattcggt
      781 cacgaaaaga accgcgcgga aatgctatct gtacaaatcg gctctgtaaa gttttttttg
      841 gcgggggaga gttaatgaca ctataggtca ccacttagta tcatttcagg cggtgcgagg
      901 ctcagagtct gcatgtgcca ccgcaagcac acgaatgtac atgtcgccaa cgtcgctgca
      961 gttgccgtac agagatgtcg agtattctac ggaacggata tcgaagcccc ttgtccagtc
     1021 tcgctgtcgg gcaagaggtt acgtgcgcgc ggcgtggcga agagattcgc gctctgtctt
     1081 cagaggattc cagtacaggc gatcaacctc tcgttcggcc ttctcttcaa tttccaactg
     1141 ggactaaact cagtcagcgg agtctgcgtt gcaagccatt tgcgtgacga attgagctca
     1201 ggtcgataga aaaccactgg gccggtctca gagatccaga ccggcccagt ggtgagacaa
     1261 actcgcatcg ggcacgagtg ttcgatacgt ggatattcgt tatcgaagca ctatttcagc
     1321 gtgtgggtga cgacgccgca gcggagtttg ggttcgaacc aggtggattt cgggggcatg
     1381 atcttgcccg cgtcggcgat cttcatcaac tgttcgatcg aggtcgcgaa catcgagaaa
     1441 gagacggcga acttgccttc gttcacgatg cgctcgagct cgttcatacc gcgaatgcca
     1501 ccgatgaagt cgatgcgctt gtccttgcgc ggatcgccga tgccgagaat cggagccagc
     1561 agattgttct gcagaatcga cacatcgagg ctctcgatcg gatcatcggc tttgtacgaa
     1621 ccggcttttg ccgtcaactt gtaccattgg cctttcagat acatgccgaa cgtcgtggct
     1681 gtggtgggct tcttctcgcc ggtcttttcg accgtgaact tctcttcgac ctttttcagg
     1741 aactgctcag gcgtgagacc gttcagatct ttcaccgcgc ggttataatc catgatgtac
     1801 aactgattgt gcggaaacaa taccgccagg aagaagttgt attcctcgtt gccggtatgg
     1861 ttcggattct tctcgcggcg caccttggca atgcgcgcag ccgctgccga ccgatggtga
     1921 ccatcggcca cgtataacgc cggcaatgta ccgaacaatt cgatgcagcg cttgtgccag
     1981 ttctgatctt tgaccaccca cagcacatgc tgaatgccgt cactggtcac gaaatcatac
     2041 tcgggcgtgc ccttggtcac ttccgcaatc agcgcgtcga tctcaggctt ggcggggtag
     2101 gtcagaaaca ccgggccggt attggcatcg gtgacatcga cgtgcttcag tcggtcttct
     2161 tctttgtcgg cgcgggtcag ttcatgcttc ttgatcttat cgtcccagta ttcctgggcg
     2221 gaacagcagg caacaagccc gg
//
LOCUS       DAORHD010000865         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59910_length_2242_cov_2.276177, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000865 DAORHD010000000
VERSION     DAORHD010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59910_length_2242_cov_2.276177"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKO06_16410"
     CDS             <1..314
                     /locus_tag="PKO06_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71289.1"
                     /translation="DNRMFKVAEDLTTTMLQNVTTGDIEYGIGVEGVPGGTTRVYSVM
                     PANNALSRIDCTDTTVTGIWNNDNLGRGDDFDFSNVWIGDVFVDSQGNVYVAYGSTQA
                     G"
     gene            332..1285
                     /locus_tag="PKO06_16415"
     CDS             332..1285
                     /locus_tag="PKO06_16415"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV71290.1"
                     /translation="MKKVVCLVIVFCVVAATSSQASPIRPHQSGSVAVYNSNGVYQKT
                     LGINPLKTDGAFVAPDHVCVDKNGNIYVSDNYAHRIVKLNAQGVFQASYGQPGGVDPG
                     ELQYPRGIVCDAQGEYLYVADASNNRIQKLKLADGTWSIVGGGGAVSTPIGIAMSPAG
                     ELFVACYGSQKMIKWNGTAWKDFKALLERPRGMAIDKDGNFFVAYGGSRAVGKFAPNG
                     DPVVPPGGAVSLLNVNQIAVDKHGNVYVPTYRDDGNTTGYRVWKLKNDLTQVSNNACN
                     TTMGMAGTSQMRSPMGIAVDSAGNLYVADTGNCRVLKFSPQ"
     gene            complement(1358..>2242)
                     /locus_tag="PKO06_16420"
     CDS             complement(1358..>2242)
                     /locus_tag="PKO06_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV71291.1"
                     /translation="PTGELLKLGGKLVKDVTGYNLLGLMIGSEGTLGIFTKVILKLMP
                     LPKAAIDLLVPFKTPEAAIAVVPKIMTSTGIIPTAIEFMDRTSLRAACTYLNESLDIG
                     EAGAMLLITVDGTDVAQVERESELVGELCQKEGAIEVYVADNRTTSERVWKIRRNIAE
                     AFKVQSPHQSLEDIVVPIAAIPQMVAGVQKLSDKWGVAIPCYGHAGDGNLHATPVMNP
                     TWSEKVWHEKLPGILKDLFELTASLGGTLSGEHGIGHKRKSYMPLVCNPATIDAMKAV
                     KRAFDPNLILNPGKIIDA"
BASE COUNT          456 a          630 c          665 g          491 t
ORIGIN      
        1 ccgataatag aatgttcaag gttgccgagg atctcacgac aaccatgctc cagaacgtca
       61 ccaccggcga catcgagtat ggaatcgggg tcgagggtgt ccccggaggc acgacaagag
      121 tatacagcgt catgccggca aacaatgcgc tttcccgcat cgattgcacg gataccacgg
      181 taaccggtat ctggaacaat gacaacctgg gtcgtggcga cgacttcgac ttctcgaatg
      241 tatggatcgg cgacgtgttt gtcgattcgc agggcaatgt ctatgttgcc tatggctcaa
      301 cccaggccgg gtaatgaaag gaaaactcga tatgaagaag gttgtctgtc tggtcatcgt
      361 cttctgcgtg gttgcggcaa cttcatccca agcctctcct attcgtccac accaatcggg
      421 aagtgtggcc gtgtacaatt cgaatggtgt gtatcagaaa acgctcggca taaatccgct
      481 gaaaaccgat ggtgccttcg tcgctcccga tcatgtgtgt gtcgataaga acggcaacat
      541 ctatgtcagc gacaactatg cgcatcgcat tgtcaagctg aatgcgcagg gggtgttcca
      601 ggcgtcatat ggccagcctg gtggtgttga tccgggcgaa cttcagtacc cgcgtgggat
      661 tgtgtgtgac gctcaaggcg agtatctcta cgtggctgac gcgtccaaca accgaattca
      721 gaaattgaag cttgccgacg gcacctggtc gattgtgggg gggggcggtg ctgtctcgac
      781 cccgattgga attgccatga gtccggcggg cgaactcttt gtggcctgtt atgggagcca
      841 gaagatgatc aagtggaacg gaaccgcctg gaaagacttc aaagcacttc tggagcggcc
      901 gcggggaatg gcgatcgata aggatgggaa ttttttcgtc gcttacggcg ggtctcgcgc
      961 cgtcggaaag ttcgctccca atggcgatcc cgtcgttccg cccggaggag cggtgtcact
     1021 gctgaatgtc aaccagatcg ccgtggataa acacggcaac gtctacgtgc caacctatcg
     1081 cgacgatggc aacaccaccg gataccgcgt ctggaagctg aaaaatgacc tgacccaggt
     1141 ttccaacaac gcgtgtaaca ccacgatggg gatggccggc acttcgcaga tgcgctcgcc
     1201 catgggcatc gccgtcgatt cggctggcaa tctctacgtt gccgataccg gcaactgccg
     1261 ggttctcaag ttctcacccc agtaacggct cgataatcaa cgcccgccac aagatcttgt
     1321 ggcgggcgtt gttgctttct gcgaccggta cgatcgatca ggcgtcgatg attttgccgg
     1381 ggttgaggat caggttgggg tcgaaggctc gtttgacggc cttcatcgcg tcgatggtcg
     1441 cggggttgca gaccagcggc atgtacgatt tgcgtttgtg accgatgccg tgttcgcccg
     1501 acagcgtacc acccagcgac gcggtcagct cgaacagatc tttcagaata ccgggcagtt
     1561 tctcgtgcca gaccttctct gaccatgtgg ggttcatcac cggcgtcgcg tgcaggttgc
     1621 cgtcgccggc atgaccgtaa cacggaatcg cgacccccca tttgtctgac agcttctgca
     1681 cgcccgcgac catctgcgga atcgccgcga tcggcacgac gatgtcttcg agactctgat
     1741 gcggactctg caccttgaac gcctcggcga tgttgcggcg gatcttccag acccgttcgc
     1801 tggtcgtgcg attgtcggcg acgtagactt caatcgcacc ctccttctgg cacagctcgc
     1861 cgaccaactc ggactctcgc tcgacctgcg cgacatcggt gccgtcgacg gtgatcaaca
     1921 gcatcgctcc ggcttcgccg atgtcgaggc tttcgttcag ataggtgcag gccgcgcgca
     1981 gacttgtacg atccatgaac tcgatggccg tcggaatgat gcccgtcgag gtcatgattt
     2041 tcggcacgac ggcgatggcc gcttcgggcg tcttgaacgg tacgagcaga tcgatggcgg
     2101 ctttcggcag gggcatcagc ttcagaatga ctttcgtgaa gatgccgagc gttccttcag
     2161 agccgatcat gagaccgagc aggttgtacc cggtgacatc cttgaccagc ttgccgccga
     2221 gcttcagcag ttcgccggtc gg
//
LOCUS       DAORHD010000866         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59979_length_2240_cov_2.654920, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000866 DAORHD010000000
VERSION     DAORHD010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59979_length_2240_cov_2.654920"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="PKO06_16425"
     CDS             complement(<1..844)
                     /locus_tag="PKO06_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71292.1"
                     /translation="MSSIIKRKGFWHIQYIENGRKLHRTTKISVADDPRKLLAKQVQR
                     DFDSANARATAGVADAPTTIADGLRRYAASKRGSVSPSWLEQVKAWSEKWIAFFASKK
                     ITKFEAIRAEHIQEFINFRQTEVSLKTVKEEVMVIRSCAKWLNTTRKVSLINYQSWPT
                     VSKTPAARKERLGAYTSEEVQALLQYFKQPRRQHWHLPLMFLAYVGCRFDEMASAQVG
                     NVRESFVKIESKKTTTSAKNQFRQVELHPKLKKALMPVIKNRKPREPIFPDLGIHHDL
                     DRVLL"
     gene            1171..1806
                     /locus_tag="PKO06_16430"
     CDS             1171..1806
                     /locus_tag="PKO06_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNV71293.1"
                     /translation="MANKTEMVIREIPATLIESRIYTIRGQRVMIDADLAGLYGVETR
                     VLNQAVKRNSERFPLSFMFQLTYEEAQNLKSQTVISSLETGTASTSHGGRRKLPLAFT
                     EHGALMLATVLKSNTAGRMSIRIIEAFVKMRQLISSNADLEKKIRELERKYDGQFRVV
                     FQALHSIINPVSRKKKPYRFHPERLIKCRKKRRPQPSPASCNQRKRRASTH"
BASE COUNT          503 a          588 c          593 g          556 t
ORIGIN      
        1 gaagcaggac acggtcaaga tcgtgatgta tgccgaggtc ggggaagatc ggctcacggg
       61 gtttgcggtt cttgatcacc ggcatcaacg ccttcttcaa cttggggtgc aactctacct
      121 gcctgaattg gttcttcgcg ctggtcgtgg tcttcttcga ttcgatcttg acgaaggact
      181 cacggacgtt gcccacttgc gctgaagcca tttcatcgaa tcgacacccg acgtatgcaa
      241 gaaacatcag gggcaggtgc cagtgctgcc ggcgtggctg tttgaaatac tgcaacagcg
      301 cctgcacctc ttcggaagtg taagccccaa gcctttcctt cctggctgcg ggcgtcttgc
      361 tgaccgtcgg ccaggactgg tagttgatca gcgaaacctt tcgggtggtg ttcagccact
      421 tggcacagct gcgaatcacc atgacctctt ctttgaccgt cttcaggctg acctcagttt
      481 gtcgaaagtt gatgaactct tgaatgtgtt cggcccttat tgcctcgaac ttggtaatct
      541 tcttcgaggc gaaaaaggct atccactttt cagaccatgc tttgacctgt tcaagccagc
      601 tggggctgac gctgcccctc ttcgatgcgg catatcgtcg aagcccgtct gcaatcgtgg
      661 tgggggcgtc tgctacccca gctgttgccc tggcattggc tgaatcgaag tcgcgttgca
      721 cctgtttggc cagcaacttg cgcggatcat cggcaactga tatcttcgtt gtcctatgaa
      781 gctttcgccc gttctcgatg tattgaatgt gccagaaacc cttgcgcttg atgatgctgc
      841 tcatgcgaac ctccgaaaat caggggatgc aaaaagggat gcaaaagtgg cgaacttcta
      901 ccattttatg cctgtttcgg caggtatgca aattgggaaa aagcctgata cagagcgctt
      961 atagccttag ggcccctggt tcgagtccag gtaggcgcat tgtctcattg gcttctgatg
     1021 ctggtgtgcg gggcgttata aagtatggtg ggtgaattgg tgggtgaaag tttgaaagcc
     1081 tcaccactgg cgtgtgatgg agttgggttg cttcaaaaac gctgacgaaa agggcgtgtt
     1141 tagcattggg tgcttcttgg gaaaccaagc atggcgaata aaacagaaat ggtcattcgc
     1201 gaaatcccgg ctacgcttat cgagagccga atatatacca ttcgcgggca gcgggtgatg
     1261 atcgacgctg accttgccgg gctatatggc gttgagacac gggtgctgaa tcaagccgtg
     1321 aagcgcaaca gtgagcgttt cccactttcc ttcatgtttc aattgacgta tgaagaagct
     1381 cagaacttga agtcacaaac tgtgatatca agtctggaga ccggcacagc cagcacctct
     1441 cacgggggta gacggaagct cccactggcc ttcaccgagc atggggcgtt gatgttggcc
     1501 actgtcctga agagcaatac agccggtcgg atgagcatca gaatcattga ggccttcgtg
     1561 aaaatgcggc aactcatctc ctcgaacgcc gatctcgaga agaagatccg cgagctggaa
     1621 cggaagtatg acgggcaatt ccgggtggtc tttcaagcgc ttcactccat catcaacccc
     1681 gtgtcccgca aaaaaaaacc gtatcggttt caccctgaaa gactgatcaa atgccgaaag
     1741 aaaagacgcc cgcaaccaag ccccgcatca tgtaaccagc gaaaacgccg cgcttcgacg
     1801 cactgatcac ggcagttcct tttcttaacc tgcctgcttc tgcttcgccc gatgagctct
     1861 gaaaagtggc ttctgtcttc ctctcccgct ttgatagtga ggccgtaaag cggtttggca
     1921 agatccagac ggtgtcttat gctgggcaac tccgcgaagc cagagacttt cccagaggaa
     1981 gctttcgaat ggtttgttgg tgcgcctgac cgtcggggtg aacccttgct ccattgtgtg
     2041 agaatttttt ggccagcgtt attcttgcct gcatacacag caggttcgaa cccaaccagc
     2101 ttatactcgc attcaatcat cactgataag ccactctacc agcacttctt acacttaggc
     2161 agattcgcgt ttctatcatt tgagtaaacc gcgacagaat tgcctccccc gcaagactga
     2221 atcttgtgaa gcctctcgcc
//
LOCUS       DAORHD010000867         2239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_59998_length_2239_cov_3.419414, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000867 DAORHD010000000
VERSION     DAORHD010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59998_length_2239_cov_3.419414"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2239
                     /locus_tag="PKO06_16435"
     CDS             <1..>2239
                     /locus_tag="PKO06_16435"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71294.1"
                     /translation="LDTLTGKLRERRELLSFVSPQVLESLSGGNLLELARGRSREATV
                     LASDLRDFTTMTETYEPRLVFALVNRHLQAMAEAIRRHGGVIDRFVGDAVFAVFYAEQ
                     GEDHVRAALKAAREMNDAHRAIQADRQANGLFGYRMGIGIETGRLLTGVLGDSETRLD
                     FTVLGDIVTRAAELESASRHGSSSRIVVSEAIREGWCEPGTYQPIPGHEKAWELVEGR
                     SSTNLPLVTHETGMARSPAITTTGTEPSAEERKSAHVGPSGALAVSPRDHSTPMVLAI
                     FLLIALSLSVAYLSWNERLKNGEVARARQVMQSAVTHLTPERILIAETRRALQGVAAD
                     VVAGKLPDEACLKRFRDRTRSLFDAAPKWQTYIGLFPEQEPIPAGSPTYTIVGSRLQC
                     LVSDHRGDQGKLPFFSEPENEFMSALAQAYILDRHAEKSDFMPELGAWKEKTFDTRGF
                     KAKDYSEIFARCLNNISWEVNVNQTVGIGCFPIFRATDEDLAPVSQASVSAELFDPQR
                     VRSRLLQTRRQMKGVLLVYLDQQAASLEHLLPFLVENHRREGYEIEIVEEGGGSLPLC
                     RNQTFQTDPELREVLRQPFAAVVETSSWLVLQEVAIPKKAYRIVLAAPKPGLFNFSAT
                     SGLSALGMAMPRSGALTSDRTNEGQSFHWFSLTFWVMFLAGLLPIYWFIDRAVRRFFD
                     QEFLSLRHHLAGHFLLAVIPVLFLVWQMLERARYESEERLNSNARMFLQDQIQRLERS
                     TGLFDA"
BASE COUNT          438 a          594 c          702 g          505 t
ORIGIN      
        1 gctggatacc ttgacgggaa aactgcgcga acgacgagaa cttctcagtt ttgtttctcc
       61 gcaggtcctc gaaagccttt ccggaggcaa tctgcttgag ttggcacgag ggcgaagtcg
      121 cgaagcgacg gtgctggctt ccgaccttcg cgacttcacg accatgacgg aaacgtacga
      181 gccgcgactg gtgtttgccc tggtcaatcg acaccttcag gccatggcag aagcgattcg
      241 ccgtcacggc ggggtcattg accgctttgt cggtgatgcg gtctttgcgg tcttctacgc
      301 cgaacagggc gaagatcacg tgagagcggc tctgaaagcg gctcgagaaa tgaatgatgc
      361 tcaccgggcc attcaggcag atcggcaggc gaatgggctg ttcggctatc gcatgggtat
      421 tggcatcgag acgggacgtc tgctgaccgg ggttctgggc gattctgaaa cccgactgga
      481 ttttaccgta ctgggtgata tcgtgacgcg ggccgcggag ctcgagagtg cttcgcgaca
      541 cggttcgtcc tcacgaattg tggtgtctga ggccattcgc gaagggtggt gcgaaccggg
      601 cacgtatcaa cccattcccg gacacgagaa ggcctgggag ctggtggaag gacggagttc
      661 cacaaacctt cctttggtca cccatgaaac cggcatggca agatctccgg caataacgac
      721 gacaggtaca gaaccttcag ccgaagaacg gaagtcggcg cacgtcggtc cgagcggagc
      781 tttggcagtc tctccaagag atcattcaac cccgatggtg cttgccatct tcctgctgat
      841 cgccctctcg ctgagtgtgg cgtatctctc gtggaatgaa cgtttgaaaa acggagaagt
      901 ggcgcgggcc aggcaggtga tgcagtctgc cgtgacccat ttgactcccg agcgtattct
      961 gatcgccgaa acgaggcgag cgttgcaggg agttgctgcc gacgtcgttg cgggaaaact
     1021 gccggatgag gcctgtctca agaggttccg tgatcgcacg aggtcgctct ttgatgcggc
     1081 ccccaagtgg cagacgtata tcgggttgtt tccggagcag gagccaatcc ccgccggctc
     1141 gccgacgtat accatcgtgg gcagtcgtct ccagtgcctg gtgagcgacc atcgtggcga
     1201 ccagggaaaa ctgccgtttt tcagtgagcc ggaaaacgag ttcatgagcg ctctggctca
     1261 agcgtacatc ctcgatcgtc atgcggagaa gagcgacttc atgccggaac tcggggcctg
     1321 gaaagagaag accttcgata cgcggggctt caaggcgaag gactatagcg agattttcgc
     1381 ccgatgtctg aacaacatct cctgggaagt caacgtgaac cagacggttg gtatcgggtg
     1441 ctttcccatc tttcgtgcaa ctgacgaaga tctggctccg gtgagccagg cttcggtgtc
     1501 ggcggagttg ttcgatccgc aacgggttcg atcacgcctg ctgcagacga ggcgtcagat
     1561 gaagggtgtg ttgctcgtgt atcttgatca gcaggccgcg tcactcgaac atcttctgcc
     1621 atttctggtc gaaaatcatc ggcgtgaagg atacgaaatc gagatcgtcg aggaaggggg
     1681 cggctccttg ccgctgtgtc gcaaccagac ctttcagacg gatcccgaac tgcgggaggt
     1741 cctgcgtcag ccctttgctg ctgtcgttga aaccagttcg tggctggtgt tgcaggaggt
     1801 tgcgatccct aagaaagcct accgaatcgt cctggccgca ccgaagccgg gtctgttcaa
     1861 tttttcggcg acttctggcc tgtcggctct tgggatggcg atgccgcgat ccggtgcgct
     1921 gaccagcgac cggacgaacg aggggcagag ctttcactgg ttcagtctga ctttctgggt
     1981 aatgtttctg gccggtctgt tgccgatcta ctggtttatc gatcgagccg tgcgacggtt
     2041 tttcgaccag gagttcctct cgcttcgtca tcacctggcg ggtcactttc tgcttgcggt
     2101 gatcccggtt ctcttcctcg tctggcagat gctggagcgg gctcgatacg aaagtgaaga
     2161 gcgcttgaac agtaatgcgc ggatgtttct tcaggatcag attcagcgtt tggagcgctc
     2221 gaccggtttg tttgacgcg
//
LOCUS       DAORHD010000868         2238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60039_length_2238_cov_4.421896, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000868 DAORHD010000000
VERSION     DAORHD010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60039_length_2238_cov_4.421896"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKO06_16440"
     CDS             <1..469
                     /locus_tag="PKO06_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HNV71295.1"
                     /translation="VALPVTTGDTAYRSARLIVADALGLEQKTFKSRLLICIPMFIIG
                     IALCQLDFKVIWKYFAWLNQSLSVFTLWAVTVWLKRQQRPWLFTAVPAVFMSVVVISY
                     IVQDTKAGFGLSMPISVAIGCLCAALFAGGSWRRATTMTPQPGSGEQTAAVSA"
     gene            complement(551..1711)
                     /locus_tag="PKO06_16445"
     CDS             complement(551..1711)
                     /locus_tag="PKO06_16445"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71296.1"
                     /translation="MAKGKVLFTYKVIIPYTESSNPRKQFKIEYVVEAPDRDTALQKA
                     EREFFAYTGYNNAAWVRMPDRTGIRIWKLLPDLPQTSQTIDELVNLLSTEDPDVVYNT
                     LKKLCELEDASAAARVQPFITHANPDLAVIAIESLGRMGDRAYLQPLIELYPHQPDPR
                     IKATILSSISRLAKQTDELHDILAAALGDADGRVRANAVEVIDHLRLASAAKLLVPLL
                     SDEDNRVRANVLKALWATHDRDTLMKSLKAMMEDANRWMRASAAFVLQHIDVEGRLEL
                     LRTLSRDPDPDVFRASWKALLSLNSADCIPYWIDYLHQHKEEDLPVVMEKIENLGSDA
                     AEALLKMIVRRDTLSPLAQRFFDRLEDRAWEEEGWWSWLQLKKKRFFSGQNG"
     gene            complement(1819..2004)
                     /locus_tag="PKO06_16450"
     CDS             complement(1819..2004)
                     /locus_tag="PKO06_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71297.1"
                     /translation="MVTPAGRDLSTSNTASRPRPVKSYASQAERLAEARQTQARTAKT
                     SQPPSPSPLGRNIDIRA"
BASE COUNT          417 a          635 c          675 g          511 t
ORIGIN      
        1 ggtggctctg cccgttacga ccggtgatac cgcctatcgc tcggcccgcc tgatcgtcgc
       61 cgatgcgctg gggctcgaac agaaaacctt caaaagccgc ctgctgatct gcattcccat
      121 gttcatcatc ggcatcgccc tctgccagct cgatttcaag gtgatctgga agtatttcgc
      181 ctggctcaat cagagtcttt cggtgttcac gctgtgggcg gtgacggtct ggctcaagcg
      241 tcagcagcgg ccctggctgt ttaccgccgt tcctgccgtg ttcatgagcg ttgtcgtcat
      301 ttcgtatatt gtccaggata cgaaagccgg ctttggtctt tccatgccga tatcggttgc
      361 catcggatgt ctgtgcgccg cgctgttcgc gggtgggtcc tggcggcggg ccacgacgat
      421 gacgccccag ccggggtcag gggaacagac ggccgctgtc tctgcgtgaa ccgaaaacgc
      481 ggggttgaca acctctccgg cgaaacgtcg aagtgaaagt ccgggtatga ttgcaggccg
      541 atgctgatga tcagccgttc tgaccggaaa agaatcgctt tttcttgagc tgcagccagc
      601 tccaccagcc ttcttcttcc caggcccggt cttcgagtcg gtcgaaaaag cgctgcgcca
      661 ggggcgaaag cgtatcccgg cgaacgatca tcttcaacag cgcttccgcg gcgtcgctgc
      721 ccagattctc gattttttcc atcaccactg gcaggtcttc ttctttgtgc tggtgcagat
      781 agtcgatcca gtagggaata caatcagcgc tgttcaagct cagcagagcc ttccaggacg
      841 cgcgaaacac gtcgggatcg gggtcgcgtg acagcgtgcg cagcagttcg aggcggccct
      901 cgacatcgat gtgctgcagc acaaaggctg cggaagcgcg catccagcga ttggcgtctt
      961 ccatcatcgc tttcagggat ttcatcaggg tgtcgcggtc atgcgtcgcc cagagtgctt
     1021 tgagtacgtt ggcccgaacg cggttgtctt cgtcactcaa caacggaacc agaagttttg
     1081 cggcacttgc cagcctcaga tgatcaataa cttcgacggc attcgctcgg actctgccat
     1141 cggcgtcgcc cagggcggcc gccagaatgt cgtgcagttc atcggtttgt ttcgccaggc
     1201 gcgagatgct tgagagaatg gtggccttga tgcgtgggtc gggctgatga ggatacagct
     1261 cgatcagcgg ctgcagatag gcgcgatcgc ccatgcgccc cagactttcg atcgcgatga
     1321 ccgcgagatc cgggttggcg tgcgtgatga acggctgcac gcgcgccgcg gccgaggcat
     1381 cttcgagttc acacagcttc ttgagcgtgt tgtagacgac gtcgggatct tcagtcgaca
     1441 gcaggttgac gagttcgtcg atggtctgcg aggtttgcgg cagatctggt aacagtttcc
     1501 agatgcggat cccggtgcgg tcgggcatgc gcacccaggc ggcattgttg tagcccgtgt
     1561 aggcgaaaaa ttcccgttct gctttctgca gggcggtatc ccgatcagga gcctcgacca
     1621 catactcgat cttgaactgt ttgcgtgggt tcgaagactc ggtgtacggg ataatgactt
     1681 tgtacgtgaa caggactttc ccctttgcca tgtgtacatg gtagcagaat cacgcgggtt
     1741 cgacaattct gtccccggca tggcatgaga gaacctcgcg ggcacgtggc gcgcgggaaa
     1801 cgaccgtttc gggacagtct aggcgcgaat gtcgatgtta cgaccgagcg gtgaagggga
     1861 gggcggctga ctcgtcttgg ccgtgcgcgc ctgagtctgc cgcgcctcgg ccagcctttc
     1921 ggcctgcgag gcatacgact tcaccggccg aggccgtgaa gcggtattgg atgtgctcaa
     1981 atcgcgacct gcaggagtca ccatggatct gccccctttc cgaaaaaacc gctctttctt
     2041 caaagatact caccgatcgc caaatgatca agtcgggttc tcaacaatca acttcttgga
     2101 agctttagcc gcgtgaagac tgcaactggt cgaaagttta accgccggcc agtttggctt
     2161 taaagccgcg cttttccagt tccagttgca gggtgtcgcg atggtcaccc tgaatctcga
     2221 tcacgttgtc gcgcacgg
//
LOCUS       DAORHD010000869         2235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60187_length_2235_cov_2.835321, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000869 DAORHD010000000
VERSION     DAORHD010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60187_length_2235_cov_2.835321"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..205
                     /locus_tag="PKO06_16455"
                     /pseudo
     CDS             80..205
                     /locus_tag="PKO06_16455"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            233..1345
                     /locus_tag="PKO06_16460"
                     /pseudo
     CDS             233..1345
                     /locus_tag="PKO06_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            1355..1912
                     /locus_tag="PKO06_16465"
     CDS             1355..1912
                     /locus_tag="PKO06_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV71298.1"
                     /translation="MSTELLAASRQHVADRAAGSDVGDTLALYLAFRLVDDVYAIDIL
                     RIREIIEYSVPTGVPMMPRSVRGVINLRGAVVPVIDLAVRFGRTATGVGKRTCIVIVE
                     TEHAGALYTLGLIVDGVNAVMEISAKSIEPAPAFGTRVNADFIAGMARVNGRFVIILD
                     ITRVLSIDDMVSITPPAMIENLKPL"
     gene            1927..>2235
                     /locus_tag="PKO06_16470"
     CDS             1927..>2235
                     /locus_tag="PKO06_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71299.1"
                     /translation="MAREIKVMIVDDSAVVRRVMSTILERDPGIRVIATAHDPIFAIE
                     KMRRDWPDVLTLDIEMPRMDGITFLGQLMAERPTPVVICSSLTTAGAETTLQALAAGA
                     V"
BASE COUNT          463 a          690 c          719 g          363 t
ORIGIN      
        1 gagacggttg cggggagggt gacgggggcg gcgggccggc tggctgcgct cgctggcctt
       61 gctggcggta ttgacggcca tgggttgggc aatctcgcgt tcgatttcgg gtccgctcaa
      121 tacggtcgtc gacgtgtccg gccgcatggc ggcaggcgac ttcgacttca agctcgacac
      181 caccgggcgg gatgaagccg gggcagtctt tcgtgccgtg atgatggtca aggaggcggt
      241 gcacgcgatg atcggcgatg ccggcgcgct gtcgaccgca gcggcgaacg gccggctgga
      301 gacgcgggtc gatgcggcca ggcaccaggg cgaatatcgc aacatcatcg aaggtctgaa
      361 ccgcacgatg gacgcagtgg tagacccgat caacgatgtc aagcgggtca tggcggcggt
      421 tgaacagggc gatctcggcc agagcatcga cgctcactat cagggcgact tcggcgccct
      481 gcagacgagc gtgaataata cgctggccaa attgtcagcg acgattgcgc aggtgaacac
      541 ctcggctggc gagctctcga acgcctcgac gcaggtatcg gccacctcgc agcttctttc
      601 gcaagcgacg tcggaacagg cggcaagcct cgaacagacg accgcagcga tggagcagat
      661 gtcggcgtcg attgcgcaga acaccgacaa cgccaagacc accgactcga tcgcgcgcca
      721 atcggcggca gacgcgctgg ctggcggcga agcggttcgt tcgacggtcg cggcgatgaa
      781 atcgatcgcc ggcaagatct cgatcatcga cgacattgcc taccgcaccg atctgctggc
      841 gctgaatgcg gcgatcgaag cggcacgagc cggcgaacac ggcaagggtt ttgccgtcgt
      901 cgcggccgag gtcaggaaac tcgccgaacg cagtcagatc gccgcacagg agattggcga
      961 actggcgagc agcagcgtcg aaaccgccga acgtgccggc agcctgttcg aaaccatgct
     1021 gccgtcgatt cgcaagaccg ccgatctggt cggcgagatc accgccgcat cggaagaaca
     1081 aacgaccggt gccgaccaga tcagtcaggc gatggcgcag ttgaataccg tgacccagca
     1141 gaacgccgca accgcagagg aactctcggc cacggccgag gagatgaacg cccaggccga
     1201 aaacctcaac gagttgatgg cgcagttcac gcttgccggc aacgacctgg ccatgccgat
     1261 gcgcccggtc agacccgcca gaccgggcaa ggcgaaacgc gattctgcgg caaatcaccc
     1321 gctgaaagat tacgaacgat tctgagggcg cgccatgagc acagaactgt tggcagccag
     1381 ccggcagcac gtggccgaca gggccgccgg ttcggatgtc ggcgacactt tggcgctata
     1441 tctcgcgttc cgcctggttg atgacgtata tgccatcgat atcctgcgca tccgggaaat
     1501 catcgagtac agcgtgccca ccggcgtgcc gatgatgccg cgctcggtcc gtggggtcat
     1561 caacctgcgc ggcgcggtgg tgccggtgat cgaccttgcg gttcgcttcg gtcgcacggc
     1621 taccggcgta ggaaagcgta cctgcatcgt catcgtcgaa accgaacacg cgggggcact
     1681 ctacaccctg ggattgatcg tcgacggcgt caacgcagtc atggagattt cagccaagag
     1741 catcgaaccc gcccccgcct tcggtacacg cgtcaatgcc gacttcatcg ccgggatggc
     1801 gcgcgtcaat ggccgcttcg tgatcattct cgacatcacg cgcgtcctgt cgatcgacga
     1861 catggtgtcg atcaccccgc ctgcgatgat tgaaaaccta aaacccctct gaaacgtgag
     1921 gcagtgatgg cacgagaaat caaggtcatg atcgtcgatg actcggccgt ggtacgccgg
     1981 gtgatgagca ccatcctgga acgcgatccc ggtatccgcg tcatcgcgac ggcccacgat
     2041 ccgatcttcg cgatcgaaaa aatgcgccgt gactggcctg acgtgctgac gctcgacatc
     2101 gaaatgccgc gtatggacgg catcaccttt cttggccaac tgatggcaga acgaccaacg
     2161 ccagtggtga tctgctcatc gctgaccacc gccggggcag aaacgaccct gcaggccctg
     2221 gcagccggag ccgtc
//
LOCUS       DAORHD010000870         2228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60476_length_2228_cov_2.943396, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000870 DAORHD010000000
VERSION     DAORHD010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60476_length_2228_cov_2.943396"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PKO06_16475"
     CDS             <1..348
                     /locus_tag="PKO06_16475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV71300.1"
                     /translation="YPDKQGTAEGRTGPAPEVVRASKLAAEKVHQALVQQLPPDLPDD
                     GLKGDSQTFIGSKQGALTGSIFSEVPVVLVEMAVLSSPQDAKFIGSAEGQERVAKALS
                     DGIISALKQISSR"
     gene            complement(395..1990)
                     /locus_tag="PKO06_16480"
     CDS             complement(395..1990)
                     /locus_tag="PKO06_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV71301.1"
                     /translation="MDKRLDAFLLVNRIGVAMLFIWAVFASMNAAFGSWSATIAAVPT
                     ISQITPVSCVVSWPVYPQYDDSTHYQVRLNGALYGSSTKGTSQKVIAQRPGYTSTIDY
                     VIYHKGKVLGVSSATQVLQCPATPVGVYADTITSTSFRLSWGDVPTAEEYLIIIDGYP
                     SQTVTAPAHQVLLDGFLPGVLLNVRVRARNASGYSYDSKPLTVQLLPPAPLQISVSEI
                     GQTSFRLSWLAATGAASYTVFRDTETVVEVGSSTLTYVVASCTPGASHTVRLQSRNAN
                     GLSDLSTETRVLMIPATPLRPMTLAVSSQSFTISWIPVLGAQGYKIWRDTEWQIGNVS
                     SQTTTATFKKGFDPGNAASITVSAYNQTGDSPQSPGVYVVLTDASGTASLTVWLLPDD
                     MEIAAWKPGSQLTPVLARIEDASAANHESRRPGPMALVVWNPERSAEQGKMLRLCTDL
                     DADVGRVGVKIQPAVSKSVRESQLFLIDQRRVVVAVYAITNAQTARQEILRAFGLQLP
                     PPLIGPELMREYRHFEELHRLGQ"
BASE COUNT          466 a          675 c          658 g          429 t
ORIGIN      
        1 tacccggaca aacaaggcac tgccgaaggg cgcaccgggc cggcccccga agtagtccgc
       61 gccagtaagc tcgccgccga aaaggtacac caggcgcttg tgcagcagct cccgcctgat
      121 ctgcccgacg atgggctgaa gggcgattcc cagaccttca tcggctcaaa acaaggggcg
      181 ttaaccggct cgatcttttc ggaagtaccg gtcgttctcg tcgagatggc ggttctgagc
      241 agcccgcaag atgcgaagtt catcggttcg gccgaaggtc aggaacgtgt ggccaaagcc
      301 ctttctgacg ggatcatctc ggctctgaag cagatttcat cccgctgatc gcgagtgccg
      361 gcggcgttcg ttcggcggtt tcgcgagctg attcttactg tccgaggcgg tgcagttcct
      421 cgaagtgacg atactctcgc atcagctcgg gcccgatcag tggtggcggg agctgcagac
      481 caaaggcgcg cagaatctcc tggcgcgcgg tctgagcgtt ggtgatcgca tacacggcaa
      541 ccacgacccg acgctgatcg atcaaaaaga gttgtgactc gcggacgctc ttcgataccg
      601 ccggttgtat tttcaccccg acccgcccca cgtcggcatc aagatcagta cacaatcgca
      661 gcattttccc ctgttccgcg cttcgctcag ggttccagac taccaaggcc attggtccgg
      721 gtcgtcgact ctcatgattc gctgctgaag catcttcgat gcgtgcgaga acaggggtga
      781 gctggctccc gggtttccac gcggcgatct ccatgtcgtc aggcaacagc cagacagtca
      841 gtgaggcggt tccagaggca tcggtcagca cgacatagac gcccggcgac tgaggggaat
      901 cgcccgtctg gttgtatgcc gaaaccgtga tgctggcagc gttccccgga tcaaaccctt
      961 tcttgaaggt agcggtcgtg gtttgcgacg acacattccc gatctgccac tcggtatctc
     1021 gccagatttt gtacccctga gcccccagga cgggaatcca ggaaatcgtg aacgactgac
     1081 tgctgacggc cagagtcatc ggccgtagcg gagttgccgg aatcatcaat acccgggttt
     1141 cggtcgacag atccgagagc ccgttggcat ttcgcgactg caagcgcacc gtgtggctcg
     1201 ccccgggggt acagctcgcc accacatagg tgagggtact cgaccccact tcgaccacgg
     1261 tttccgtatc acgaaacacg gtgtatgaag cggctcccgt tgccgccaac caggacaaac
     1321 gaaagctcgt ctgcccgatc tcactgaccg agatctgcag gggagccggc ggcaggagtt
     1381 gaactgtgag cggtttcgag tcgtaggaat acccggaggc attccgcgcc cgcacgcgca
     1441 cgttcagcag aactccgggc agaaagccgt cgagcaaaac ctgatgcgcc ggtgcggtta
     1501 ccgtttgcga cgggtaccca tcgatgatga tcagatactc ttctgccgta gggacatctc
     1561 cccaggacaa ccgaaacgac gtcgaggtaa tggtgtcggc atagactcca accggggtcg
     1621 ccgggcactg caacacctgg gtggccgagg aaactccgag cactttgcct ttgtggtaga
     1681 tcacgtaatc gatcgtgctg gtgtaccccg gacgctgagc gatgaccttc tgcgatgtgc
     1741 ctttcgtcga cgacccgtat agtgcgccgt tcagccgcac ctgatagtgc gtggagtcat
     1801 cgtattgcgg atacaccggc catgagacga cgcacgagac gggagtgatc tgcgagatcg
     1861 tggggaccgc ggcaatcgtg gccgaccagc tgccaaaagc ggcgttcata ctggcaaaca
     1921 cagcccagat aaacagcata gccacgccga tccggttcac cagcagaaac gcatccaatc
     1981 gcttgtccat acatctcccc cttgtcatac gtccccggca ctatcggaag atgtcacggt
     2041 ttctttaacg aacgtgctca gtcttcgggt ttgagcagac cacggtcgcg cccgagccag
     2101 taggccagca aatacccgac gggggcgacg acggcaccgt catcggcgtt gtacacaacc
     2161 tttcgcggag gttcctgcca gggaaagatg tgagggcaac gctcgttcat gggaaacacc
     2221 cggtcagc
//
LOCUS       DAORHD010000871         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60590_length_2225_cov_3.812903, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000871 DAORHD010000000
VERSION     DAORHD010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60590_length_2225_cov_3.812903"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2225)
                     /locus_tag="PKO06_16485"
     CDS             complement(<1..>2225)
                     /locus_tag="PKO06_16485"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV71302.1"
                     /translation="FGNDRILLVAFPVQAIDAALVRMLTQTERRLLALPNVQTVLSPV
                     SPLRTQFGIASEAAIDKFLAEKKQLDRYLENLKKTTALEKLIISPAWNVGGMVIRLNQ
                     ETGDLVGPTIQQIHRVLADCMPVGTKLTGVPEITRLILDMTRRDQKLFSPLTILLTAI
                     VLFLLFRGWFGLLTPLLAICSAFIWTKGLLIAQGHAINFVTSILPPMILSIALTYCIH
                     ILTDYFEASRDLDGFDPRVLADSLRHVAYPIMLSTLTTVIGFGSLLYTSIESIGQFGA
                     YAAIGTTVSMVLALTVLAAGIAARRKTGHVMPSTIKSLEASIDRLSRAMIRNPGRVWL
                     VVFLLLVVSGYGLYKLPIETSLIRYLPDDHDIQEANRFVEENLSGIVPVELFLESDGR
                     RFTEPDIVHRVRRFQEAVASVPYLDKSLSYVNLVQDFDRMFSGEPNHVPPTEEEVRDY
                     LTFYAPAPASGALTPDELEEMAEASRTLLLELQAATDTDSRLEMASSADLLAKTATPA
                     TTTLFIAGGMLGELLSPDARTAHVSMRYRDRTSREMFAGFQMIEALAKKEFADSGIRA
                     TITGRAPLWAEVSEIIVWNEITSFGFSLLIITCIISGYFRSLKIGIVSMIPNTLPMIY
                     TYGIMGLTGTTFNTVTGMIASIAIGLAVDDTIHIICQFQHELEKDGNEQEALVRTMVH
                     KGRATVFASVILCTGFSVLALSSFGPTRYFGVFISVGVAAALICELLITPVALYTFKP
                     IP"
BASE COUNT          548 a          625 c          628 g          424 t
ORIGIN      
        1 ggaatcggct tgaacgtgta gagggcgacc ggggtaatca acaactcaca gatcagcgcg
       61 gctgcgactc cgaccgagat gaacacgcca aaataacggg tcggtccgaa actcgagaga
      121 gcgagaaccg aaaatccagt gcagagaatg acgctggcga ataccgttgc ccgccctttg
      181 tgtaccatcg ttcgaaccag cgcttcctgt tcgttaccgt ctttctcgag ttcatgctga
      241 aactgacaga tgatgtgaat ggtgtcgtcg accgcaagac cgatggcgat actggcgatc
      301 atgccggtga cggtgttgaa agtggtaccc gtgagaccca tgatgccgta ggtatagatc
      361 atgggcagcg tgttcggaat catggaaacg atgccgatct tcaacgaccg gaagtacccg
      421 gaaatgatgc aggtgatgat cagcagcgag aaaccaaaac tggtgatttc attccagacg
      481 ataatctccg acacctcagc ccacagcggg gcccgaccgg tgatggttgc gcgaatacca
      541 gaatcggcaa actccttttt cgccagggcc tcgatcatct ggaaccctgc aaacatctca
      601 cgtgaggtgc gatcgcggta acgcatcgag acgtgggccg ttcgggcatc aggcgacagc
      661 agctctccga gcattccccc ggcgatgaac agtgtcgtcg ttgccggagt cgcggttttc
      721 gccaacaaat cagctgaaga cgccatctcc aaacgggaat ccgtatccgt agccgcctgc
      781 agctcaagca gaagagtacg actggcttcc gccatttcct cgagttcgtc gggtgtcagc
      841 gcgcctgaag ccggtgccgg agcataaaag gtgagataat cacgcacttc ttcttccgtg
      901 ggcggaacat ggttgggctc ccccgaaaac atacgatcga aatcctggac gaggttcaca
      961 tacgaaaggc tcttgtccag gtacggcaca ctcgcgaccg cctcctgaaa ccgccgaaca
     1021 cgatgcacaa tatcaggctc ggtgaatcgc ctgccatcgc tctccagaaa tagctcgacc
     1081 ggtacaatgc cactcaggtt ttcctccacg aatcggtttg cttcttggat gtcatggtca
     1141 tcaggcagat atctgatcaa gctcgtttca ataggcaact tgtacaatcc gtagccggaa
     1201 accaccagca gcagaaacac taccagccac accctccccg gattacgaat catggctcga
     1261 gacaggcgat cgatagaggc ctcgagactc ttgatggttg agggcatgac atgcccggtc
     1321 ttgcgccggg cggcgattcc ggcagccaga accgtcagcg cgagcaccat ggaaacggta
     1381 gtgccaattg cggcatacgc accgaactgc ccgatcgact cgatggaggt atagagaaga
     1441 gacccgaagc cgatcacggt tgtcagcgtc gagagcatga tcggatacgc gacatgccgc
     1501 aggctgtctg cgagaacccg ggggtcgaaa ccatcgagat ctcgcgatgc ctcgaagtaa
     1561 tcggtcagaa tgtggatgca gtaggtgagc gcgatcgaaa gaatcatcgg gggcagaatc
     1621 gacgtgacga agttgatcgc gtgcccctgg gcgatcaaca gacctttggt ccagatgaag
     1681 gcgctgcaga tggccagcag aggagtgagc aacccgaacc acccacgaaa cagcagaaac
     1741 agcacgatcg ccgtcaacag aatcgtcaga ggcgaaaaca gcttctggtc gcgtctcgtc
     1801 atgtcgagaa tcaggcgggt gatctccgga actccggtca gtttcgtgcc aaccggcata
     1861 cagtccgcca gcacccggtg aatctgctga atggtcggcc cgacgagatc gcccgtttcc
     1921 tgattcagac gaatcaccat gccgccaaca ttccatgcag gcgaaatgat caacttctcg
     1981 agcgccgttg tctttttcag attttcgaga tagcgatcga gctgcttctt ctcggccaga
     2041 aatttgtcga tcgccgcttc agaggcaata ccgaactggg tgcgcaacgg gctgaccggc
     2101 gaaagaaccg tctgcacgtt cggcagtgcg agcagacggc gctccgtctg agtcagcatg
     2161 cggaccagag cggcgtcaat ggcttgtacc gggaacgcaa ccaggagaat gcgatcattc
     2221 ccgaa
//
LOCUS       DAORHD010000872         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60594_length_2225_cov_3.322581, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000872 DAORHD010000000
VERSION     DAORHD010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60594_length_2225_cov_3.322581"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PKO06_16490"
     CDS             complement(<1..1453)
                     /locus_tag="PKO06_16490"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV71303.1"
                     /translation="MKRWFLPISAVMAILTVIGVLVHRPATTAQEISGGMFRSYLTSE
                     PANLDPARGVDVSEGLVQAKIFDGLVRYDENMKLVGDLAESWEIIEEGRSYVFHLRPG
                     VLFHDGTPLTAADIVFSLTRLLDPAVRSPRAWILEKVAGARDRLDGKVETVSGIAAID
                     EKTVKITLAEPFAPFLSMLSMPAAYILPVAHQAAIADRSFFDKPAGTGPFKLIGRERD
                     SYLRLSANPDYFGDKPGVGTLEMRVIPESMKAEMEFETGNLDILQLHPSNYERFKARG
                     EEAGKVTDIPAMNIFYVGFNNQAAPFNDIRVRKALNYLVDREALIKAVFKGRGVPARG
                     SIPPGILGYSDQVNGYGYDPAEGRRLLKEAGYDAKKPLKFSLYQKSSQAAFEITRLLQ
                     GELKKHGIEVSLKPMEWSALKDAIDKGEAEAFYLSWYGDYPDGENFLYPLFHSKNWGS
                     GGNRARFKDENVDTMLEKALKIQDQAKRAEAYDQ"
     gene            complement(1483..1959)
                     /locus_tag="PKO06_16495"
     CDS             complement(1483..1959)
                     /locus_tag="PKO06_16495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV71304.1"
                     /translation="MKIGLISDTHTHSLDAWILDAFSGVDLILHAGDMISDHVLLELQ
                     AIAPTLGVRGNCDPFFPGLPPIRRLKTKNGEIVMAHRPEHVMGLVTPETFLVVHGHTH
                     EPRFEPEAHFWVANPGSPSSPRGGNRPSVLIVEVAAQGNAPICKGEFKFPPSVADG"
BASE COUNT          471 a          689 c          603 g          462 t
ORIGIN      
        1 tctgatcata cgcctcggcg cgcttggcct gatcttgaat cttcagagct ttttccagca
       61 tggtgtcaac gttttcatcc ttgaaccggg cgcggttgcc gcctgaaccc cagtttttcg
      121 aatgaaacaa cggatacaga aagttctcgc cgtcgggata gtcgccatac cagctgagat
      181 agaaagcctc cgcttctccc ttgtcgatgg catccttgag cgcgctccat tccatgggct
      241 tcaggctcac ttcgataccg tgctttttca attccccctg caacaggcgg gtgatctcaa
      301 aagcggcttg agaagatttc tgatacaggg aaaatttcag cggtttcttc gcgtcatagc
      361 cggcttcttt cagcaggcga cggccttcgg caggatcata gccatacccg ttcacctggt
      421 cggaataccc gagaatgccg gggggaatgc tgccccgggc gggcacgccg cggcctttga
      481 acacggcttt gatcaaagct tcgcgatcaa caagatagtt cagagccttg cgcacgcgga
      541 tgtcgttgaa cggcgccgcc tgattgttga acccgacgta gaagatgttc atggccggaa
      601 tgtcggtcac ttttccggct tcttcgccac gcgccttgaa ccgttcataa ttcgacggat
      661 gcagctggag aatatcgaga ttgccggtct caaattccat ctccgccttc atgctctcag
      721 gaatcacccg catttcgagt gttcccacac cgggtttatc gccaaaataa tcaggattgg
      781 cggacaggcg cagataactg tcacgttcgc gaccgatcag cttgaacggc cctgttcccg
      841 cgggcttgtc gaagaagctg cggtcagcaa tagcggcctg gtgggcgacc ggcagaatgt
      901 acgccgccgg catcgacaac atgctgagga atggtgcgaa aggttcagcc agcgtgattt
      961 tcacggtctt ctcatcgatc gcggcaatac cgctcaccgt ttcaactttg ccgtcgaggc
     1021 ggtcgcgagc accggcaacc ttttcgagaa tccaggcgcg cggagaacgc acggccggat
     1081 cgagcaggcg ggtcagagaa aacacgatat ccgcggccgt cagcggtgtg ccatcatgga
     1141 acagcactcc cgggcgcagg tgaaaaacgt acgatcgccc ctcttcgatg atttcccagc
     1201 tttcggcgag atcgccgacc agcttcatgt tctcatcgta gcgaaccagg ccatcgaaaa
     1261 tcttggcctg aaccagccct tcgctgacat cgacgccgcg agccgggtcg agattcgcgg
     1321 gttcgctggt cagatagctg cgaaacatcc ccccggaaat ttcctgggcc gtagtagccg
     1381 gccggtgcac gagcaccccg atcacggtca gaatggccat taccgctgaa atgggaagaa
     1441 accaacgttt catgtgcacg ccctcgttga tggagttaca tgttatccat cggccacaga
     1501 gggcgggaac ttgaattctc ccttgcagat cggtgcattt ccctgcgccg cgacctcgac
     1561 aatcagcacg gaagggcggt tgccgccgcg aggtgacgag ggactgcccg gattggccac
     1621 ccagaaatga gcttcgggtt caaagcgcgg ctcgtgagta tgaccatgca ccaccagaaa
     1681 cgtttccggc gtcaccaggc ccatcacatg ctcggggcga tgcgccatga cgatttcccc
     1741 gttcttggtt ttcagccgcc tgatcggcgg cagtcctggg aaaaacgggt cacagtttcc
     1801 ccgaaccccg agcgtgggag caatggcctg taactccagc agcacgtggt cactgatcat
     1861 gtcccccgca tgcagaatca gatcgacccc ggaaaaagca tcgagaatcc aggcatcaag
     1921 agagtgggtg tgggtgtccg agatcagacc gattttcatc tctgttgttc ctgtgtcgtc
     1981 aatcttgaca agagcgtacc agatacctac attatatcgc catgactacc ccgacaacag
     2041 gtccaaagcc accctcgttt tccgcttcgc caaaacccgg tgctactcca gcgtccggct
     2101 ctcccgctcc agcgcagggc cccgccaaac caggcactcc gtcgccatcg ccggcccccg
     2161 ccccaaaacc cggaacgcca gtggccgcca cgccacccgc cgctgttcag gggctcgccg
     2221 acccc
//
LOCUS       DAORHD010000873         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60629_length_2224_cov_3.464269, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000873 DAORHD010000000
VERSION     DAORHD010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60629_length_2224_cov_3.464269"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKO06_16500"
     CDS             complement(<1..242)
                     /locus_tag="PKO06_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71305.1"
                     /translation="MFAVADFFKKLRDMPFAEIFETQLDGYDDLTYVWDILARIQKRF
                     IARRVKQDILGTVEPGAWLQGADIFIGKGTRVEAGA"
     gene            563..1009
                     /locus_tag="PKO06_16505"
     CDS             563..1009
                     /locus_tag="PKO06_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71306.1"
                     /translation="MNQDFRDLLAAFNDHHVEFLVVGAHAMAAHGHVRATKDMDVWIR
                     PSSDNAKRAIQALTAFGAPLQGLTAEDLSTPGVVFQIGVPPLRIDILTAIDGVTFEEA
                     WPNRVISTFADQPAAVLSRNDLIRNKRASARKQDLADVEVLEKIGS"
     gene            complement(989..1201)
                     /locus_tag="PKO06_16510"
     CDS             complement(989..1201)
                     /locus_tag="PKO06_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71307.1"
                     /translation="MDRTKVRKLSLHDPEQDLAYMEGTPEERILAVWELTKEAYSLSP
                     EHDVERRLQNTIVTVRRRTSSTADFL"
     gene            complement(1308..2018)
                     /locus_tag="PKO06_16515"
     CDS             complement(1308..2018)
                     /locus_tag="PKO06_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71308.1"
                     /translation="MPHLKSLSRYRRLTNSVLRLSNPRNMHPVAMNLSVRVAESRGHG
                     LMPFFMKSVGEVLRAHPEFAVLNSVLDRGWWRSRVVCFDDLSFSVAVEKPFEGENVVA
                     VYVLHDVMRRGLDEITAEYAALKTLPIEEFPLFRKMRWLLSVPDFFHLPLFKLAALFP
                     RDYDSYGSMGLSNLGRGHIQTFFPSTSKTCIFGLGGLIERDGLRELTVNLVFNHFVVD
                     GALCARFLEALKTRLELS"
BASE COUNT          493 a          640 c          619 g          472 t
ORIGIN      
        1 atcgcgcccg cttcgacgcg cgttcccttg ccgatgaaga tgtccgcgcc ctgcagccag
       61 gcccctggtt cgaccgtgcc gagaatgtcc tgcttcacgc ggcgcgcgat gaagcgcttc
      121 tggatgcggg ccaggatgtc ccagacgtag gtcaggtcgt catacccgtc gagctgagtc
      181 tcgaagatct cggcgaacgg catatcgcgc agtttcttga aaaagtcggc aacggcaaac
      241 atcgtgtcgt gtctcctcgt cgttggttcg cccgtattct acccattgtc gcccaaaaac
      301 acaaaacccc cgaaaccgaa agatcaaaca atgattggtg cccccttcac gtaacgacca
      361 gcaagaatgc gtttcagcaa tactgttgtg atatactgag tgatcatggc aaacacgatc
      421 cgcccttcca tttcctcgtg caagatcatc cggcctggcg cgacgtcgga ccttgatcga
      481 gactggcttg tcatgccaat tccagagaga atcgaggcgg tctggactct cacgcgtgcg
      541 tgcatggcct ggaacaattc cgatgaacca agacttcaga gatctattag ctgcattcaa
      601 cgaccatcac gttgagtttc tcgtggtggg tgcgcatgcc atggcggctc atgggcatgt
      661 tcgagcgaca aaagacatgg acgtctggat ccgcccgtcg agtgacaatg ccaaacgagc
      721 gattcaagcg ctcacggcct tcggcgcacc tctgcagggt ttaaccgcag aggatctgtc
      781 gacccccggc gtcgtttttc agatcggggt tccgccactg cgaatcgaca ttctgacggc
      841 tatcgatggt gtgacgtttg aagaagcctg gccgaaccgt gtgatttcca cctttgctga
      901 tcagccggcg gcggtgcttt cccgcaatga tctgattcgc aacaagcgtg cttcggcgag
      961 aaaacaggac ctggcagacg tcgaggtctt agagaaaatc ggcagttgaa gaggtccgcc
     1021 ggcgaacggt gacgatcgtg ttctgaagtc ttcgttcaac atcgtgttcg gggcttagcg
     1081 agtaggcttc cttggtcagt tcccagacag cgagaatgcg ctcttccggc gtgccttcca
     1141 tataggcgag gtcctgctcg gggtcgtgca atgaaagctt gcgcaccttg gttcgatcca
     1201 tcttcatatc cccatgatat cacgcgtcaa catggcaatt aagcgccttg aacagggctg
     1261 aaaacgcacg tttcaggcgc tgtcggcaga aaccgacggg ggttgcgtca gcttagttcg
     1321 aggcgggttt tcagggcttc gagaaaacgg gcgcagagag cgccgtcgac gacgaaatgg
     1381 ttgaagacca ggttgacggt cagctcacga agtccgtcgc gttcgatcag gccacccagc
     1441 ccgaagatgc acgtcttcga cgtgctcgga aagaaggttt ggatgtgccc ccgaccgaga
     1501 tttgacagcc ccatgctgcc gtacgagtcg taatcgcgcg gaaacagtgc agcgagcttg
     1561 aatagtggca ggtgaaagaa atcgggcacc gacagcagcc accgcatctt gcgaaacagc
     1621 ggaaactcct cgataggcag ggttttcagc gcggcatact cggcggtgat ttcatcgaga
     1681 ccgcgccgca tcacgtcgtg cagaacgtag accgccacaa cgttctcgcc ttcgaacggt
     1741 ttttcgacgg ccaccgaaaa cgacagatcg tcgaaacaga cgactcgact gcgccaccac
     1801 ccgcggtcga gcacactgtt caataccgcg aactccgggt gcgcccgcag aacctcgccg
     1861 accgacttca tgaaaaacgg catcaatccg tgacctcgcg actcggcaac ccggacgctc
     1921 agattcatcg ctaccgggtg catgttccgc ggattcgaga gccgcagcac gctgttcgtc
     1981 agccgccgat accgcgacaa cgacttcagg tgaggcatgt tcgttccccc aattcgttgt
     2041 ttgcctgagc tttcttcata actctgagtg atggtgacgc tgttttcaca gcgcttcctt
     2101 gtctttcgcg ggcatcagca gatgttctag agaacagacg ttgagcaaaa actctccgga
     2161 acgccctttc caggattctt gaatccgggc gaaccagaag gggcgggtaa tcatgtcatc
     2221 ataa
//
LOCUS       DAORHD010000874         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_60959_length_2217_cov_3.087882, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000874 DAORHD010000000
VERSION     DAORHD010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60959_length_2217_cov_3.087882"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /locus_tag="PKO06_16520"
     CDS             complement(<1..1471)
                     /locus_tag="PKO06_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNV71309.1"
                     /translation="MSAPTNPRIEANLKTLRYPERITLDAVLSRKVLEQMQKEVVEPT
                     RQKLTIQVGMGTCGLASGAQKVWDYLTNLKNDGKLDANLRKVGCNGYCATEVLVDIRK
                     PGKPQVTFGSVDVKKMEKIYESYLKGDKIPMEYVVGISENFPGEQNFDVPAEFNGVKK
                     FKDLDFFKKQFRIVLKNCGNIDPESVDEYLARGGYQATAKALKMTPRQVVDELIASGL
                     RGRGGGGFPTGKKWEFAFNQKSDQKYIVMNADEGDPGAFMDRSVLEGDPHRVIEGMII
                     GGYAIGATIGYIYVRAEYPLAIERLNIAIAQCHKYGLLGKNILGSGFDFDLRLKMGAG
                     AFVCGEETALIASIEGRRGMPKPRPPFPAVAGVFGKPTVINNVETFANVAEIIDRGAK
                     WFAAIGTEKSKGTKVFALTGKIAISGLIEVPMGITLREIIFDVGGGIPGGLAFKAAQI
                     GGPSGGCLPTPCLETPIDYDSLIQAGAMMGSGGLVVMDEK"
     gene            complement(1468..1986)
                     /gene="nuoE"
                     /locus_tag="PKO06_16525"
     CDS             complement(1468..1986)
                     /gene="nuoE"
                     /locus_tag="PKO06_16525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV71310.1"
                     /translation="MNTGCQCGGSDQLTTIKLPELQEILTRYHANKREYLIPILQETQ
                     GAYGYLSKPAMVQIAQHVGVPVSKVYGVATFYNQFKLTAPGKHIISICRGTACHVRGS
                     DQILRAFESELSIKAGQTTKDQEFSLDIVACIGSCSIAPVITADGEFHGRLTTKDVVR
                     ILTDIRNGGAKK"
BASE COUNT          441 a          606 c          678 g          492 t
ORIGIN      
        1 tcttttcatc catgaccacg agcccgcccg agcccatcat ggcgccggcc tgaatcaacg
       61 agtcataatc gatgggggtt tcgagacagg gggtgggcag acaaccgccc gacgggccac
      121 cgatctgagc cgctttgaaa gcgagaccgc cgggaatgcc gccaccgacg tcgaagatga
      181 tctcgcgcag cgtgatgccc atcggcactt cgatcaagcc tgagatggcg atcttgccag
      241 tcagggcgaa caccttcgtg cctttcgact tttcagtacc aattgccgcg aaccactttg
      301 cgccccggtc gatgatctcg gccacattgg caaacgtttc gacgttgttg atgacggtcg
      361 gcttgccaaa cacgccggca acggcgggga acggagggcg gggtttgggc atgccgcgcc
      421 tgccttcgat gctggcgatc agagcggttt cttcgccgca gacaaaagcg ccggcaccca
      481 tcttgagccg gagatcgaag tcgaatccgc tgccgaggat gtttttgccg agcaggccat
      541 acttgtggca ctgcgcgatg gcgatattca ggcgttcgat cgcgagcgga tactcggcgc
      601 gcacgtagat gtagccgatg gtggctccga tggcgtaacc gccgatgatc atgccttcga
      661 tgacgcgatg cgggtcacct tcgagaaccg accggtccat gaacgcaccc gggtcgcctt
      721 catccgcatt catgacgatg tacttctgat cagacttctg attgaacgcg aattcccact
      781 tcttgcctgt cgggaacccg ccgccgccgc gaccgcgcag cccggaggca atcagttcgt
      841 cgacgacctg tctcggggtc atcttgagcg ctttcgcggt cgcctgatac ccgccgcgag
      901 ccagatattc gtcgaccgat tcagggtcga tgttgccgca gttcttcagg acgatgcgga
      961 attgcttttt aaaaaagtcc aggtccttga acttcttgac accgttaaat tcagcgggaa
     1021 cgtcgaagtt ctgttcgcca ggaaaattct cagagatgcc gacaacgtat tccatcggta
     1081 tcttgtcgcc cttgaggtag gactcgtaga tcttttccat tttcttgaca tcgacagacc
     1141 cgaaggtgac ctgtggcttg ccgggtttac gaatgtcgac cagcacctca gtggcgcagt
     1201 acccgttgca gccgaccttg cgcaggttgg catccagttt cccatcattc ttcagattcg
     1261 tgaggtagtc ccataccttt tgtgcgcctg atgcgagacc gcaggtgccc atgccgacct
     1321 ggatggtcag cttctgtcgg gtcggctcaa cgacctcttt ctgcatctgc tcgagcactt
     1381 tgcggctgag cacggcgtcg agcgtgatgc gttcgggata gcgcagggtc ttgagattgg
     1441 cttcgatgcg gggattggtg ggagcactca tttcttggcg cctccgttgc ggatatcggt
     1501 gagaatgcgg acgacgtcct tggtggtcag gcggccatgg aactcgccgt cagcggtaat
     1561 gaccggggcg atcgagcacg agccgataca ggccacgatg tcgagcgaaa attcctggtc
     1621 tttggtggtc tggccggctt tgatggaaag ttcggattca aaagcccgca gaatctggtc
     1681 ggagccgcgc acatggcagg cggtaccgcg acagatcgag atgatgtgct tgccgggggc
     1741 ggtgagtttg aactggttgt agaacgtggc gacgccgtac actttgctga cgggaacgcc
     1801 gacgtgctgg gcgatctgga ccatcgccgg tttggaaagg tagccatagg ccccctgggt
     1861 ttcctgcagg atcggaatca ggtattcgcg cttgttggca tgatagcgag tcagaatttc
     1921 ctgcagctcg ggcagtttga tcgtggtgag ctgatcactg ccaccgcatt gacaaccggt
     1981 gttcatggag ttcctccgag aagctcgtgg ggcacgtggc cccgtgatgc cctgactccg
     2041 ggtttggtga acggcaacga aggggtcagg gttcgggcgc aaaggacgtt caattcatat
     2101 tataacgctt tgcgaaaaat atcacaagct aaatgtgaag aaagtgatgt tgctggacga
     2161 taaggggggg ggaaggaacc gccagaattc gccgggccac actcagcaat gttgaca
//
LOCUS       DAORHD010000875         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61005_length_2216_cov_2.838501, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000875 DAORHD010000000
VERSION     DAORHD010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61005_length_2216_cov_2.838501"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PKO06_16530"
     CDS             complement(<1..854)
                     /locus_tag="PKO06_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71311.1"
                     /translation="MANRSKSLNACSCASRPRFCRGMLLVIGFLFAFSRAGVADTRAG
                     DPLVQPCLESLEGVNREIAQGASSRKVLLKLQQLKTTVKRAAPELNAGEKAKYQVLLE
                     DTIEPAIVELAQNGDAPVTARARQNVRRAKSAVVQPAPVQPLISGKIDFETARGKKCS
                     WDLGTLVQSLQRNPSKPSAAATRPATAADIATVRSFITSGKGTWLPNLRQWFRFKHRP
                     MTPETISRRVAGFLEDYVSRPENLPRLGRDLGLKIPMKARIASVVIRTMRWCGLKVTI
                     PGGDPETI"
     gene            complement(1112..1753)
                     /locus_tag="PKO06_16535"
     CDS             complement(1112..1753)
                     /locus_tag="PKO06_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71312.1"
                     /translation="MTLGHLASRKAAQKPLWGEVGTSAQRVKIDFDTPWETGVTQKLQ
                     LAAIVALVIVFAGLVGGPKGGKPHPEMLYLLPIPLAFAGWYWWRRRSVSEYYIADLAS
                     RQLLFCREEHDVRQEKPCCSFEMVFCVGVSAVRHSTKGGHHYEYHVALVDRHARITVM
                     SDPSRTIPEVEQKALMLAELLGCRFEPTQGMTELVAKLNPGSDPTISRRHLAG"
     gene            complement(1852..>2216)
                     /locus_tag="PKO06_16540"
     CDS             complement(1852..>2216)
                     /locus_tag="PKO06_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71313.1"
                     /translation="AKRAREATVSQNDTLASTPKPIVQAAVPTAQPVLPVSALPAQTR
                     TPELATTAPEPIFRGWRWFFLGLWLVTISPMMPWLYWLDLFIIYRLWTNGAWFRALLL
                     IGWAFAFPLAAVILGGIH"
BASE COUNT          454 a          658 c          664 g          440 t
ORIGIN      
        1 tcgatggttt cggggtcccc gccgggaatg gtgaccttga gtccgcacca acgcatggtc
       61 cgaatcacga ccgaggcgat tctggccttc atggggattt tcaaaccgag atccctgccc
      121 aggcgcggaa ggttttcggg tctggacaca tagtcttcca gaaaaccggc gactcgcctg
      181 gagatggtct cgggagtcat cgggcgatgc ttgaaccgga accactgacg gagattgggc
      241 agccatgttc ctttccccga agtgataaag gatcggacgg tcgcgatgtc cgccgccgtc
      301 gcaggccgcg tggctgcagc cgagggtttc gacgggttcc gctggagcga ttgcacgagg
      361 gtcccaagat cccacgagca cttcttcccc cgcgcggtct cgaaatcgat ttttcctgag
      421 atcagaggtt gaacaggggc aggctggacg accgccgact tcgcccgacg gacgttctgt
      481 cgcgcccggg cggtgacagg agcatcgcca ttctgcgcca gttcgacaat ggcaggttcg
      541 atcgtatcct cgagcagaac ctgatacttc gccttctcgc ctgcgttcaa ttccggtgcc
      601 gcgcgtttca cagtggtttt gagctgctgc agtttcagaa ggactttccg ggacgaggcc
      661 ccctgggcga tttcccgatt cacgccctca agactctcga ggcacggctg aaccaaggga
      721 tcaccagcgc gcgtatctgc aactccagct cgcgagaacg cgaacaggaa tccgatgacc
      781 agaagcatcc ccctgcagaa acgcggcctt gatgcgcatg aacaggcgtt caagcttttc
      841 gatcggttcg ccatgagcac tctcctccat tgcatcacat cattctccat cgattaaaca
      901 ggactgtcag cgttctgtca acgaccgtct cgtccgcatt gctctcgttg tgcccacctc
      961 accaccagca agagagcttc cgtttcctga tgtcccaggt gccgtaaact ggtcgccgag
     1021 gcgattcagt gccccttcaa ggaaggcgaa cgcgtcgaga aactgcgtat catgggattg
     1081 taacgtcgca gatcagggca cctgagcact gtcatcccgc caggtgtcgg cgcgagatgg
     1141 tcgggtctga accggggttc agtttggcta ccaattcggt catgccctga gtcggttcga
     1201 agcggcaacc cagcagttcc gcgagcatca gcgctttctg ctccacttcc ggaatcgtgc
     1261 gtgacgggtc gctcatgaca gtgatgcggg catgtcgatc cacgagcgca acgtggtatt
     1321 cgtagtgatg cccacccttc gtggaatggc gaactgctga aacgccgacg caaaagacca
     1381 tctcgaacga acagcaaggc ttctcctgcc gaacgtcatg ctcttcgcgg cagaacagca
     1441 gttgcctgga agcaagatcg gcgatgtagt attcagagac ggatcggcgg cgccaccagt
     1501 accaaccggc gaacgcgaga ggaatcggca agagatagag catctccgga tgcggctttc
     1561 ccccctttgg gccgccgacc aacccggcga agacgatcac gagagccacg atcgcggcaa
     1621 gctgcagttt ctgtgttacg cctgtttccc agggagtgtc gaagtcgatc ttcacacgct
     1681 gtgccgaggt gccgacctca ccccacaagg gtttttgcgc ggcttttcgt gaggccagat
     1741 gcccgagggt cacaggggtg ctcctgcgcg gatcgccggt acgtggagta ttcatgagca
     1801 agtcacgacc atcagcgtgt ctgttgttga aaagcaggtg tgcatcagcc gtcagtgaat
     1861 gccacccagg atgacggcgg caagcggaaa ggcgaaagcc cacccgatca ggagcaacgc
     1921 acgaaaccag gcaccgttcg tccacagacg atagatgatg aacaggtcga gccagtagag
     1981 ccatggcatc atcgggctga tcgtgaccag ccagaggccc aggaagaacc agcgccagcc
     2041 gcggaagatc ggctccggag cggttgttgc caactcaggg gttcgagtct gggccggaag
     2101 cgcactcaca ggcagtactg gttgcgctgt cggcacagcg gcttgtacta ttggtttcgg
     2161 ggttgacgcg agcgtgtcat tctggctcac cgtcgcttcg cgggcccgct ttgccg
//
LOCUS       DAORHD010000876         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61239_length_2210_cov_3.556845, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000876 DAORHD010000000
VERSION     DAORHD010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61239_length_2210_cov_3.556845"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1458)
                     /locus_tag="PKO06_16545"
     CDS             complement(310..1458)
                     /locus_tag="PKO06_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71314.1"
                     /translation="MKIRQLLLVLILSLSPLYGIELKDILVSETPEELEIKQSKFLSI
                     AEKDLLQYEQIVRRLDQAESSGGANVTEYRRLRAEFDAYLTRRLNLIRKIFLAFRKEF
                     TRVVDFIEPVGTDRLPAFSGLIAVLDDSIDEAKMKGSKDKWRDLKDRVNALQTTLNKP
                     GVRQAYLDKLVLSSTEASETVFEAKTRLLHRWLLEGRRTEARPWVLELEKTHAEKALC
                     LALLSHYWLEEYNARRGLPNPQELLEKELAKKFGTGAVTVKVSGADDLQKAFLYYLEA
                     LNKGYPPELEIPGLLPAYWERIRDKFNKIHPYWVFPDHFPGYRDFMKELASACQAVVE
                     NVPFKKYVSPAYRLLSDMYTYWPSKAGPKIIDFQEIMRLNRIAGNKKL"
     gene            complement(1638..>2210)
                     /locus_tag="PKO06_16550"
     CDS             complement(1638..>2210)
                     /locus_tag="PKO06_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HNV71315.1"
                     /translation="LVAPVAIATMVEHIGDVLAISETVKKNYIESPGLHRTLIGDGVA
                     TSLSALFGGPPNTTYSENTGVLALTKVFNPLVMRVAAVFAIILGLVPKLGGLISTIPE
                     SVVGGISIVLFGMIAAIGARSLIENKIDLTQSRNLIIVAVILVLGLGGAVLPVKIATI
                     SFSLEGMALAAIVGILLNKLLPETTDSETV"
BASE COUNT          459 a          596 c          672 g          483 t
ORIGIN      
        1 cagtcctgcg tcgctggatg ctgaattccg gtgacctgaa acgtggagac ggggcatgag
       61 cgagtggttt ctctttgtcc tggtcggctt ctttctcgga gtatggcttc tcggcctcat
      121 ggtggctccg tcgcttgccg gtctattctg ctgatggaat cgatcatgga ccatatgtcc
      181 tacctgatcg aagaaggaaa ccacaccctg ctgtatctac agaagcgttt accttcgaaa
      241 ataacgagct gaagcaaacc gccccggact gcacgagtgc ttgtccgggg cggttggttt
      301 gggtgagggc tagagcttct tgttgccggc gatgcggttg aggcgcatga tttcctgaaa
      361 gtcgatgatc ttggggccgg ctttgctggg ccaatacgtg tacatgtcac tcagtaaccg
      421 gtatgccggg ctgacatatt ttttgaacgg cacgttctcg accacggcct ggcaggcact
      481 ggccagttcc ttcatgaaat cgcgataccc cggaaaatga tcggggaaga cccagtaggg
      541 gtgaatcttg ttgaacttgt cccgaatgcg ctcccagtaa gcgggcagca ggccgggaat
      601 ctcgagttcg ggcggatacc ccttgttcag ggcttcgaga taatagagaa acgccttctg
      661 cagatcatca gcaccagaga ctttgacggt gacggcaccg gtaccgaact tcttggccag
      721 ttccttttcc agcagttcct ggggattggg cagcccgcgc cgggcgttgt attcctcgag
      781 ccagtagtgc gaaagcagag ccagacagag agctttttcc gcatgggtct tttcgagttc
      841 gagcacccag ggccgagctt cggtgcggcg accttcgagc agccagcgat gcagcaggcg
      901 ggtctttgct tcgaacacgg tttccgaggc ttcggtggaa gagagtacga gtttgtcgag
      961 ataggcctga cgcacgcccg gcttgttcag agtcgtttgc agggcgttca cgcggtcctt
     1021 gagatcgcgc cacttgtcct ttgagccttt catcttcgct tcatcgatcg agtcatcaag
     1081 aacggcaatc agaccggaaa aggccggcaa gcgatcggta ccaacgggtt cgatgaaatc
     1141 gacgacgcgc gtaaactcct tgcggaacgc caggaagatt ttgcgaatca gattcagacg
     1201 ccgtgtcaga tacgcgtcaa actcggcgcg cagacggcga tactcggtaa cattcgcgcc
     1261 accgctcgat tctgcctggt cgagccgtcg cacgatctgc tcatactgaa gcaggtcttt
     1321 ctctgcgatg ctcagaaact tgctctgctt gatttcaagc tcttccgggg tttcgctgac
     1381 gagaatgtcc ttgagttcga tgccgtagag cgggctcagg ctgaggatca gaacgagaag
     1441 cagttggcgg atcttcatgc attcctcccg gggatggcgt gatcaccaac cagtatacac
     1501 gatagcgaaa tccgatgggt aggatccgac ggcgagtttt cccgccggaa cctgaaaggt
     1561 gccgttggtt caccccgtct gcgcgatcga tctgaaggtc acgcgaacgc cgggtcaggg
     1621 gggcaggact gtgccgacta cacggtttct gaatcggttg tttcggggag cagtttgttc
     1681 aagaggatcc cgacgatggc ggcgagtgcc atgccttcga ggctgaagct gatcgtggca
     1741 attttcaccg gcagaacggc accgccgaga ccgagcacga gaatcacggc cacgatgatg
     1801 agattccgcg actgcgtcag gtcgatctta ttctcgatca gactgcgagc gccgatggcg
     1861 gcaatcatac cgaacaacac gatcgagatg cccccgacaa cggactcggg aatggtcgag
     1921 atgagcccgc cgagcttcgg aaccagaccc agaatgatgg cgaagacggc cgcgactctc
     1981 atgaccagcg gattgaagac cttggtcagg gcaagcacgc cagtgttttc cgaataggtc
     2041 gtgttgggcg ggccgccgaa cagggccgag agagacgtgg ccactccgtc gccgatcaac
     2101 gtgcgatgca gcccgggcga ctcgatgtag ttcttcttca ctgtttcgct gatcgccaga
     2161 acgtcaccga tgtgctcgac catggttgcg atggcgaccg gagcgacgag
//
LOCUS       DAORHD010000877         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61304_length_2209_cov_2.691736, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000877 DAORHD010000000
VERSION     DAORHD010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61304_length_2209_cov_2.691736"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="PKO06_16555"
     CDS             <1..899
                     /locus_tag="PKO06_16555"
                     /inference="COORDINATES: protein motif:HMM:NF016201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNV71316.1"
                     /translation="MENSPKLRKDENDWMRRIMQIVRDTSLYFQPQIRTKILNEGWAS
                     YWHEVLFMEDPAMRTHEFEWSRINAGVVSLPRVGLNPYAIGLRLFKYLEAKADRGMLT
                     YEFEKVKKVDDRKIFDKKTGAGRDFIFKLRQYGSDANAIREFVDQDFCNEFMLFVVGK
                     RINPERQVWEYFVKSRKAEDFRRQMEDNMYHPPHITVHRENTEKNGGLHLVHHFEGSE
                     LVREYIHNTMLGIEFLWGDKVFLETHEVDESQLEQAHQEGPQQGVSAPAAEPEFRFQK
                     VVYTMENRKLTRQVFDQYLTAR"
     gene            905..2194
                     /locus_tag="PKO06_16560"
     CDS             905..2194
                     /locus_tag="PKO06_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71317.1"
                     /translation="MANNALSRIIDNLTHDHSHKPLALDVFLNNLNRNGELVLRNIYQ
                     LFHDMVLFYLREGKSEPPFDGEAAGFLNYNLDRIFIQETEKPFFADRLFTNRLVNLAK
                     SFRASATRNKIFVFQGPAGSGKSTFLNNLLAKLEEYTRSENGQLYEVLWRLPIERTTG
                     DESVENGRDNRPWLSGQKPGADNVFEVPCPNHDNPILMIPKQYRLELLREVIPEGEFK
                     KKLFKHRQYQWVFKDDPCTICGSLFSALMERYAIKDIFGMITARRSFFNRKQGLGISV
                     YNSGDDLEKNVVRTNESIQAFLGDFFQDSNKVKYLFSGYSCTNQGIRALMDLKARNIQ
                     RFLDLHGIISDEVHKVSDLEERIKSMFIVLMNPGDLEHIDEEYKEKDSSIDLSLKDRI
                     HEIQVPYVLDYATEIKIYVNTFGEQIRLRFMPASWNS"
BASE COUNT          548 a          632 c          576 g          453 t
ORIGIN      
        1 tcatggagaa ctccccgaaa ctgcgcaagg atgagaacga ctggatgcgc cggatcatgc
       61 agatcgtgcg cgataccagc ctgtactttc aaccgcagat tcgcaccaag attctcaacg
      121 agggttgggc cagctactgg cacgaagtgc tgtttatgga agacccggcc atgcgcacgc
      181 acgagttcga gtggagtcgc atcaacgccg gcgtcgtctc gttgccccgt gttggtctga
      241 atccgtatgc gatcgggttg cgactgttca agtatcttga agccaaagcc gaccgcggca
      301 tgctgacgta cgagttcgag aaagtgaaga aagtcgatga tcgcaaaatc ttcgacaaaa
      361 agaccggtgc cggtcgcgat ttcatcttca aactacgaca gtatggcagt gatgccaacg
      421 cgattcgcga gtttgtcgac caggacttct gtaacgaatt catgctgttc gttgtcggca
      481 agcgcatcaa cccggagcgg caggtctggg aatacttcgt aaaaagccgc aaagccgaag
      541 atttcaggcg gcagatggaa gacaacatgt accatccgcc ccacattacc gtgcaccgcg
      601 agaacaccga gaagaacggc gggctgcacc tggtacatca ctttgaaggc agtgaactcg
      661 tccgcgagta tatccacaac accatgctcg gcatcgaatt tctgtggggc gacaaggtgt
      721 ttctcgaaac ccacgaggtc gacgagagcc agctcgaaca agcccaccag gaaggcccgc
      781 agcagggcgt cagtgccccg gcggcagaac ccgagttccg gtttcagaag gtggtctaca
      841 ccatggaaaa ccgcaagctc acccgccagg tcttcgatca gtatctcacc gccaggtaaa
      901 caacatggcc aataacgcgc tgtcccgcat catcgacaat ctcacgcacg atcacagcca
      961 caaaccgctg gctctcgatg tctttctcaa caacctgaat cgcaacggcg aactggtact
     1021 gcgcaacatc tatcagttgt ttcacgacat ggtacttttc tatctgcgtg aagggaaaag
     1081 cgaaccgccc ttcgacggcg aagccgccgg gtttctcaac tacaacctcg accgcatctt
     1141 catccaggag accgagaagc ccttcttcgc tgatcgtctg ttcaccaacc gcctggtcaa
     1201 tctcgccaag tcgtttcgcg ccagcgccac ccggaacaag atcttcgtgt ttcaaggccc
     1261 ggccggcagc ggcaagagca cctttctcaa caaccttctc gccaaactcg aagagtatac
     1321 gcgctctgaa aacggccaac tgtatgaagt gctgtggcga ctgcccatcg agcgaacgac
     1381 gggcgacgaa tcagtggaga acgggcgtga caaccgtccg tggctttcgg ggcagaaacc
     1441 cggtgccgac aacgtgttcg aagtcccctg tccgaatcac gacaacccga tcctgatgat
     1501 tcccaaacag taccgcctgg aactgctccg cgaggtcatt cccgagggcg agttcaagaa
     1561 gaagctgttc aagcatcgtc agtatcaatg ggtcttcaag gatgatccgt gtacgatctg
     1621 cggcagtctg ttttccgctc tcatggagcg ttacgcgatt aaagatatct tcggcatgat
     1681 tacggcccgc cgctcctttt tcaatcgcaa acagggtctg ggcatttccg tgtacaactc
     1741 gggagacgat ctcgagaaga acgtggtgcg cacgaacgag tcgattcagg ctttccttgg
     1801 cgatttcttt caggacagca acaaggtcaa gtatctgttt tcgggctact cgtgcacgaa
     1861 tcagggcatt cgcgccctga tggatctcaa agcccgcaac attcagcgat ttctcgacct
     1921 gcacggcatc atcagtgacg aagtgcacaa ggtcagcgat ctggaagagc gcatcaagtc
     1981 catgttcatc gtcctgatga acccgggcga tctcgagcat atcgacgaag agtacaaaga
     2041 gaaagattcc tctatcgacc tgtcgttgaa agaccgcatt cacgagatcc aggtgcctta
     2101 tgtgctcgat tacgccaccg aaatcaagat ctatgtcaat acgttcggcg aacagatcag
     2161 actgcggttc atgccggcca gctggaactc gtagtcgaag tcggtcacc
//
LOCUS       DAORHD010000878         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61334_length_2208_cov_3.183465, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000878 DAORHD010000000
VERSION     DAORHD010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61334_length_2208_cov_3.183465"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..451)
                     /locus_tag="PKO06_16565"
     CDS             complement(98..451)
                     /locus_tag="PKO06_16565"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV71318.1"
                     /translation="MMIAISAKGDTLDAPLDPRLGRAERFLLIDSENESFTVMTPQTA
                     QMSHGAGIQTAQALLKAGVKVVISGDCGPKAFQVFQSAGVPVYSSSGGTIRDVLNAWK
                     KKELPVIGEPGPGGH"
     gene            complement(448..975)
                     /locus_tag="PKO06_16570"
     CDS             complement(448..975)
                     /locus_tag="PKO06_16570"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNV71319.1"
                     /translation="MTQYNARVLEHFYHPRGVGRLEPFDGIGTLGDPDCGDYLEVTIR
                     LSEDHERLTAVAFRVKGCPAAIATSSMMTELATNATVEQSLALTDQQIIEALDGLPPG
                     KEHCSLLAVRGLQLAVQDAVLRRLLKKAGMVASDEEFDQLKASGGLEHFFHRCDGSCE
                     ALAEPSDSQKGNENP"
     gene            complement(1042..1464)
                     /locus_tag="PKO06_16575"
     CDS             complement(1042..1464)
                     /locus_tag="PKO06_16575"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNV71320.1"
                     /translation="MPGRDGTGPQGQGARTGRGMGPCGTRIEATAEQLTPFPGMRFGR
                     RFGFGAGRGLGRRQRMTTRQRRLLNQETLDMADRLEPTETVTTSDLHRLTDILNALQK
                     DVQMLHDRLDRLQERRTTPQPPADPKVETDESTPTETE"
     gene            complement(1526..2011)
                     /locus_tag="PKO06_16580"
     CDS             complement(1526..2011)
                     /locus_tag="PKO06_16580"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV71321.1"
                     /translation="MTVPNHFDVNRRRCLQDAHAETLGDRFAPPWFDPMSWQFTETGE
                     LHGTLEEGLQTEGYRQLVHGGAIAALIDAAMTHCLFGHGIVALTAELKVHYRKPLLCK
                     RSIQIQTGIEQVIGDQLYRLKARVMQEQETRTEAFGAFFRPLPGDPGSTYVCGTPPDP
                     R"
     gene            complement(1989..>2208)
                     /locus_tag="PKO06_16585"
     CDS             complement(1989..>2208)
                     /locus_tag="PKO06_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71322.1"
                     /translation="PSKRPASVSFPASIRDLPKNWLRPISMGIWSRFLRPALRSRTIR
                     LELDGAALPTGMAGVINIAACHDGTKPL"
BASE COUNT          413 a          622 c          686 g          487 t
ORIGIN      
        1 acgctggtct tgccggtgcc gcccttgcca ctggcgatag ccagccatgg ccggtgtccg
       61 ttcacggaca ggcgcatctg agaagtggcg tggcccatca gtgccctcca ggaccgggtt
      121 cgccgatgac cgggagttcc tttttcttcc aggcgttcaa gacatcacga attgtgccac
      181 ctgaagatga gtagacgggg actccggcgc tctgaaacac ctggaaggct ttgggtccgc
      241 agtctcccga gatcacgacc ttgacgccgg ccttcaacag agcctgtgcc gtctgaatgc
      301 cagctccgtg cgacatctgt gccgtctggg gagtcatgac ggtaaacgac tcattctcac
      361 tgtcgatcaa cagaaaccgc tcagctctgc cgagtcgggg gtcgagaggg gcatcaagcg
      421 tgtcaccctt ggcggaaata gcgatcatca tgggttttcg tttcctttct ggctgtctga
      481 tggttcagcg agagcttcgc aagagccgtc gcaacgatgg aaaaagtgtt caaggccgcc
      541 tgaagctttg agctgatcaa attcttcgtc actcgcaacc attccggcct ttttcagcag
      601 tcggcgcagt accgcatcct ggaccgccag ctgtaaacca cgaacggcca gcagggaaca
      661 atgctctttc ccaggaggca ggccgtcgag ggcctcgatg atctgctggt cggtcagagc
      721 cagcgattgc tctacggtcg cattggttgc cagctcggtc atcatgcttg aggtggcgat
      781 ggcggcgggg catcccttga cccggaaagc gaccgctgtc agcctctcgt gatcttccga
      841 gaggcgaatg gtcacttcga gatagtcgcc gcagtccgga tccccgaggg tgccgattcc
      901 gtcaaaaggc tccaggcgac cgacgccacg tgggtggtag aagtgttcga gaacgcgagc
      961 gttgtattga gtcatgcggt gatgatggtt gtgggaagga agcgcttgca gtcatgcggc
     1021 tgcaagcgcc agtgctgatt gttactcggt ttccgtcgga gtcgattcgt cggtctcgac
     1081 tttggggtcg gcaggcggtt gcggcgtggt cctgcgttcc tgtagccggt ccagtcgatc
     1141 atgaagcatc tgaacatcct tctgcagggc gttcagaatg tcggtcaggc gatgcagatc
     1201 actggtggtg accgtttctg tgggttcgag acggtcagcc atgtcgaggg tttcctgatt
     1261 cagaagtcgc cgttgtcggg tagtcatgcg ctgtcgacgt cccaggccgc gccctgctcc
     1321 gaagccaaac cgacgaccaa atctcattcc ggggaatggc gtcagctgct cagcggtcgc
     1381 ttcgatcctg gtgccgcagg gtcccattcc gcgcccggtt ctggcccctt ggccctgagg
     1441 tccggttcca tctcttcctg gcataagaat tctccttgcg tgatgatgta ggtatgagga
     1501 ggagaagcgc tccggagtcg cttgtctacc tcggatctgg tggtgtcccg cagacgtacg
     1561 tgctgcctgg atcgccgggt aaaggtcgga agaatgcccc gaacgcttcg gttcgggttt
     1621 cctgttcttg catgacgcga gctttcaaac gatagagctg gtcgccaatg acctgctcga
     1681 taccggtctg aatctggatt gaccgcttgc agagcaatgg cttgcgataa tgcactttca
     1741 attcggcggt gagagcaacg atgccgtgtc cgaacaggca gtgcgtcatg gctgcatcga
     1801 tcagagccgc gatcgctccc ccgtgaacca gctgacgata tccctcggtc tgaagcccct
     1861 cttccaatgt gccgtgaagt tcgccagttt cggtgaactg ccaggacatc gggtcaaacc
     1921 agggcggggc aaaacgatct ccgagagtct ctgcatgcgc atcttgcagg cacctgcggc
     1981 gattcacgtc aaagtggttt ggtaccgtca tggcatgccg cgatgttgat gacaccggcc
     2041 atgcccgttg gcaaggccgc gccgtcgagt tcgagtcgga tggtgcgcga gcgcagagca
     2101 gggcgcagga accgagacca gattcccatc gagataggcc tgagccagtt cttcggcaga
     2161 tcgcggatcg atgccggaaa tgacacggat gccggacgct tcgagggt
//
LOCUS       DAORHD010000879         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61341_length_2208_cov_2.963307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000879 DAORHD010000000
VERSION     DAORHD010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61341_length_2208_cov_2.963307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="PKO06_16590"
     CDS             complement(<1..1848)
                     /locus_tag="PKO06_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV71323.1"
                     /translation="MSHTIKLVVIADKTDNLVALHAIVRDALPTTTVFSATTGPTGLD
                     LAVAEDPDVILLDIVLPGMDGFEVCRRLKADDRTKDIPVVFFSALKTDTASRVKALEA
                     GAEGFLDTPPEPIELTALIRAMVKVKAANRSQRREKEQLEALVAERTREVRDSEQKFR
                     FLHESAGLGIGYYTPDGVVISYNQAAARYMNGRPEDFEGRSIHDLFPEEAAQVYLGRI
                     KKAIQSEIPQVYEDMVRLPKTSLCFLSTFARVVDSSGKVIGVQIISDDITERKRVEEA
                     LRESQAVFRALFEKSADAILLIDESRVFVECNQAALNLLKMTREAFLNKPPVMISPEF
                     QPNGRRSDEAAQDMMDQAYEKGLHRFDWTCVDSEGGEFIVEVSLMPIVINGKTMLHTT
                     WRDITARKQLELERQKFFQLAESSSEFIGMCDLAMIPIYVNPAGRRMVGLADMASACR
                     VKVQDYYFPEDQAFIRENFFPRLLKEGQGSIEIRLRHFQTSQPIWMFAHYFVIRDSRD
                     QAVGWAIVSRDITARKQAEEEQRKLQAQLAQAQKMESVGRLAGGVAHDFNNMLGVILG
                     HAELAMESIGPNEPLRSTLEEILKAAERSADLTRQLLAFARKQTVSPRVI"
BASE COUNT          443 a          671 c          627 g          467 t
ORIGIN      
        1 gatcactctc ggcgagaccg tctgcttgcg ggcaaaagcc agcagttggc gcgtcagatc
       61 agcggaacgc tcggccgcct tgaggatctc ttccagggtg gaacgcaggg gctcgttcgg
      121 cccgatgctt tccatggcca gttccgcatg acccagaatg accccgagca tgttgttgaa
      181 atcgtgtgcc acgccacccg ccagccggcc caccgattcc atcttctgag cttgcgcgag
      241 ctgcgcctgc agcttcctct gttcctcctc ggcctgcttg cgagccgtga tgtcgcgact
      301 gacaatggcc cagcccacgg cctgatctct ggaatcgcga atcacaaaat agtgggcgaa
      361 catccagatc ggctgactgg tctggaagtg acgcaatcga atctcgatac tcccctggcc
      421 ctctttgagc aggcgcggga aaaagttttc cctgatgaag gcctgatctt cgggaaagta
      481 gtaatcctgc actttcaccc ggcaggctga ggccatgtcc gccagcccga ccattcgacg
      541 gcccgccgga ttcacataga tcggaatcat cgccaggtcg cacattccga tgaattcact
      601 gctgctttcg gccaactgga agaacttctg ccgctcgagc tcgagttgtt tacgcgccgt
      661 gatatcccgc cacgtcgtgt gcagcatcgt ttttccgttg atgacgatcg gcatcagcga
      721 gacttcaacg atgaactcgc cgccttccga atcgacgcag gtccagtcga agcggtgcag
      781 gcccttctcg taggcctgat ccatcatgtc ctgcgcggct tcgtcggaac gcctgccgtt
      841 cggctggaac tcgggcgaaa tcatgaccgg cggcttgttg agaaacgcct cacgagtcat
      901 cttgagcagg ttgagcgccg cctgattgca ctcgacgaag acgcggctct cgtcaatgag
      961 cagaatcgcg tcagccgatt tctcgaacag ggcgcggaaa actgcctggc tctcccgcag
     1021 cgcctcctcg acccgcttgc gctcggtgat atcatcggaa atgatctgga cgccgatcac
     1081 cttgccgctg gaatcgacca ccctggcaaa ggtgctgaga aaacagagac tcgttttcgg
     1141 cagtcgcacc atgtcttcgt acacctgcgg aatctctgac tggatggcct ttttgatcct
     1201 acccaagtag acctgggcgg cctcttccgg aaaaagatcg tgaatcgagc ggccctcaaa
     1261 atcctccggc ctgccgttca tgtatctggc ggcggcctgg ttgtatgaaa tcacgacacc
     1321 atcgggcgtg taatagccga tgcccagccc ggccgattcg tgcagaaaac gaaacttctg
     1381 ttcgctatcg cgcacctcac gggtgcgctc agccaccagc gcttccagtt gttccttttc
     1441 tctccgctgg ctgcgattgg ccgccttgac cttgaccatc gcccggatca gcgcggtcaa
     1501 ctcgatcggc tccgggggag tgtccagaaa gccctcggct ccggcctcca aggccttgac
     1561 ccgactggcg gtatcggtct tgagcgccga aaagaacacg accggaatat cctttgtccg
     1621 gtcgtcggcc ttgagcctgc ggcagacctc gaagccgtcc atgccgggca ggacgatgtc
     1681 gagcagaatc acatcggggt cttcggcaac agccagatcg agacccgtcg ggccggtggt
     1741 ggcggaaaaa acggtggtcg tgggtaacgc atcgcgcaca atcgcatgga gagcgaccag
     1801 gttgtccgtt ttgtccgcta tgacaaccaa cttgatcgtg tgactcatgt gcgtggaacc
     1861 tctcaatgac acatttcctg tgcgtttccg cggcaatggg gcaacggaga ttgccatgga
     1921 agtcctggac ccgaatggtc acgggggtat tcttcaaggc gctgcacgga atggaacccg
     1981 accgcaccca ccgtttccgt tttccgcctc cacactagca acacgaacgg tcggtgtcaa
     2041 ttgccacctt ttcgcggcaa ggtcatatga ttctgtcctg tattcttctt ggcgtgagcg
     2101 agacgtgtca gccggtcggc aagagacgga atgagcccag gaaaacccac gcgagagatt
     2161 gaaatgacgc agagtcagag aagggggtct tgcaatttgg cgaggggt
//
LOCUS       DAORHD010000880         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61455_length_2205_cov_4.219535, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000880 DAORHD010000000
VERSION     DAORHD010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61455_length_2205_cov_4.219535"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..1605)
                     /locus_tag="PKO06_16595"
     CDS             complement(103..1605)
                     /locus_tag="PKO06_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV71324.1"
                     /translation="MRHHFFTPLFLTVFLFFAQHLNAATQILETDCATASSGSTTSVL
                     AANIGDSVPEPLANATADDIEPAPPLTSETLTLAQAVQEALRANPDLEIVRERLEASR
                     AQIDQARAAFFPRLTASLQYSERESPVSTLGFSGLGGRGGDIYNVQYTDALNLNLPLD
                     VGGKLKAGLKRARVNRDKAGVDLDTTISDVILGTLDACLNHLKAVALLETAREKREAL
                     ESHRAIVLQRLANDLALKTDLLKAEVQLESSRESELMAKNAIENTRSAINQILGRPIV
                     EHLTIASDTSLALDIPPLEELRTRAFTANPALRAVRQQVSVFKASLKAEQAQHRPNLT
                     LTGSTGRASRDFPPRDNAWSIGGTLDFKVFDGGLQSARVHEAAIRLREAEAQEEKIRQ
                     SILVQMQIAYSQFETALSRVEINRKAADLAREELRLSDLQLREGLLTFTDYLDNQAEF
                     TRARVAAIVAEFDLLLARLKLLHLSGGLTRAFADQLQTSQARSAGQLATP"
     gene            complement(1853..>2205)
                     /locus_tag="PKO06_16600"
     CDS             complement(1853..>2205)
                     /locus_tag="PKO06_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71325.1"
                     /translation="DRWYLEVVIRRRDMQSFSNRIDIVLTPDPTVPGRKVEPLILVGD
                     YNDTQKLAEQFASRDADREVLTRRLESIARQLRELYPTLDAGERQTLRTALGGTPALA
                     RDLQSLETFEQLHR"
BASE COUNT          420 a          592 c          741 g          452 t
ORIGIN      
        1 ttctcggcgg ttcctgacgc aaagatgcca gagtcttcat ggcgacgaca ccgctgatcg
       61 tgagcagaat tccaacgatg atggagatga gatgcgtgcg gctcatggtg ttgccagctg
      121 tccggccgag cgagcctgac tggtctggag ttggtcggcg aatgctcgcg tgagcccccc
      181 ggacaaatgg agcagtttga gacgggcgag aagcagatcg aattcggcga cgatcgccgc
      241 gacccgggcg cgggtgaact cggcttgatt gtcgaggtag tcggtgaagg tcagcaggcc
      301 ctcgcggagt tgcaggtccg aaagtcgcag ttcctcgcgg gccaggtctg ctgccttgcg
      361 attgatctcg acgcgcgaga gcgcggtttc aaactgtgag taagcgatct gcatttgtac
      421 gagaatgctc tgtcggatct tctcttcctg ggcttctgct tcacgcaggc gaatcgccgc
      481 ttcgtgaacc cgggcacttt gcaggccacc gtcgaagacc ttgaaatcaa gggtcccgcc
      541 gatgctccag gcgttgtccc gcggtggaaa gtctcgtgag gcgcgacccg tgctgccggt
      601 cagcgtcaaa ttcggtcgat gctgggcctg ctcggctttc agggaggctt tgaaaacgga
      661 gacctgctgg cgcacggccc gcagtgccgg attcgcggtg aaggcccggg tgcgcagttc
      721 ttcgagaggc ggaatatcga gagcgagaga cgtatcagaa gcgatagtca ggtgttcgac
      781 gatcggacga ccgaggatct gattgatggc actgcgagtg ttttctatgg cgttctttgc
      841 catcaattcg ctctcgcgtg acgattcgag ttgtacttcg gccttgagaa gatcggtctt
      901 gagagcgaga tcgttggcca gccgctgcaa aacgatggcg cggtgactct cgagtgcctc
      961 acgcttttcc cgtgcggttt cgagcagggc gacagcctta agatggttga gacaggcgtc
     1021 gagcgtgccg agaatgacat cgctgatcgt ggtgtcgaga tccaccccgg ccttgtcccg
     1081 gttcacgcgc gcgcgtttca agccggcctt caacttcccg ccgacgtcga gagggaggtt
     1141 caggttgagg gcatcggtat actgcacatt gtagatgtcg ccccctcggc cgccgaggcc
     1201 gctgaagccg agcgtcgaca cgggtgactc gcgctcggaa tactgcaacg aagctgtcag
     1261 gcgggggaag aatgcggccc gggcctgatc gatctgcgcc cgcgaggctt cgagacgttc
     1321 gcggacgatt tccagatcag gattcgcccg caatgcttcc tggactgcct gagccagcgt
     1381 cagggtttcc gatgtcaggg gcggggcagg ttcgatgtcg tcagcagttg cattggccag
     1441 tggttccggt acggaatcgc cgatgttcgc ggcaagaacg gaggtcgtcg atccggaaga
     1501 cgcggtcgcg caatcggtct cgagaatctg ggtcgcggcg ttcagatgct gcgcgaagaa
     1561 caggaatacc gtgaggaaca ggggagtgaa gaagtgatga cgcataacaa ccttcctgta
     1621 ggacgaatcc gaaaaggagc cctaattggt actattttct cacgcatgac gtcaatggct
     1681 gcacgaacca gcccgcaggc agactttcat gtgttccaga gagcgtgcga cagtctgacg
     1741 aaaacccgct ctacagagtg tttcccgcgg caaacgggta gcttcgtctg gctggtccga
     1801 aagaaaaagc ccccggctgg tgccgggggc tttttctgaa ccagggaaac aatcagcgat
     1861 gcagttgctc gaaggtttcg aggctctgca ggtcgcgcgc cagggcggga gtgccgccga
     1921 gtgccgtgcg cagggtctgg cgttcgccgg cgtcgagggt cggatagagc tcgcgcagtt
     1981 ggcgggcgat cgattcgaga cggcgggtca gcacttcgcg gtccgcatca cgtgaggcga
     2041 actgttcagc cagtttctgg gtgtcgttgt aatcgccgac caggatgaga ggctcgactt
     2101 tgcggcccgg aaccgtcgga tcgggggtga gcacgatgtc gatgcggttc gagaagctct
     2161 gcatgtcgcg gcgtcggatc acgacttcca gataccagcg gtctt
//
LOCUS       DAORHD010000881         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61516_length_2204_cov_2.724988, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000881 DAORHD010000000
VERSION     DAORHD010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61516_length_2204_cov_2.724988"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1273)
                     /locus_tag="PKO06_16605"
     CDS             complement(251..1273)
                     /locus_tag="PKO06_16605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNV71326.1"
                     /translation="MNLLAAFFISYFFHLGIIWFSKKKRLFLDDQEKRQKVHTRIVPR
                     IGGLGIFLACVLVVDDPFGRKLLIAAVPAFLAGFFEDLLSNISPKIRLAVISLSGVLA
                     IALLNAVVIDFEFAQIPWPLAAAFTLLMISGITNAINIIDGLNGLASGFSILALVFFS
                     LAAYTAGDSSLALLFCKFGLATLAFFVLNFPKPYIFLGDSGAYLLGFTVVISGISLIM
                     RNPAISSYFPFLVLFYPVFEVLFSMHRRYRKGVPMMSPDKLHFHTLLFKRTALSNSAS
                     TALLLAANGILCVIGLLFLRQSWVQIALVLAFIGIYTSAYRSLVRFKTRRVTEGTKRS
                     PSPRVS"
     gene            complement(1281..>2204)
                     /gene="gmd"
                     /locus_tag="PKO06_16610"
     CDS             complement(1281..>2204)
                     /gene="gmd"
                     /locus_tag="PKO06_16610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV71327.1"
                     /translation="HDYRLHYGDMTDSTNLIRIIQEVQPDEIYNLAAQSHVQVSFETP
                     EYTANADGLGVLRLLEAIRILKLEKKTRFYQASTSELYGLVQETPQKETTPFYPRSPY
                     AAAKLYAYWITVNYREAYGLYACNGILFNHESPLRGETFVTRKITRAVCRIKEGLQKK
                     LYLGNLSSKRDWGHARDYVEAMYLMLQQPKPEDFVIATGVTVTVREFVEMAFREVGIN
                     ITWKGQGVDEKGVNAKTGETLVEVDPRYFRPTEVELLLGDATKAKTKLGWKPKTNLAE
                     LVKDMVAKDMKESQRDEYLKTGGFTTFHYFE"
BASE COUNT          518 a          606 c          626 g          454 t
ORIGIN      
        1 ggggaaatgc tcacttttcc tgcgtgtgct cgggcttttc gactcacccg cgtcagaccg
       61 ctccatgccc actgagtgcc ggccaatcgc ttcgcgtgat gacaccgttc gacatccatc
      121 atggacgtcg aacggtgtca tcgactctcg ctcttggggc actcaggccc actccacttc
      181 tcttccttta cgggagccca aacaccctct gcgccaacga atatttccca tttaccctag
      241 atgcggggat ctacgaaacg cggggtgatg gggagcgttt ggtgccttcg gtgacgcgac
      301 gggttttgaa gcgcacaaga gagcgatagg cgctggtata gatgccgatg aaagcgagca
      361 cgagagcaat ctggacccag ctctgtctga ggaacagcag gccgattacg cacaggatgc
      421 cattcgccgc gagcaacagg gcggtcgagg cggagttcga cagcgcggtg cgtttaaaga
      481 gcagggtgtg aaagtgcagt ttgtccgggg acatcatcgg cacacctttt ctgtaccggc
      541 gatgcatcga gaacagcacc tcaaacaccg gatagaagag cacgagaaac ggaaagtacg
      601 acgagatcgc cggattgcgc atgatcaaac tgattccgct gatgaccaca gtaaagccca
      661 gcagatacgc gccgctatcg ccgagaaaga tgtacggttt cggaaagttg agtacgaaga
      721 acgccagcgt ggctaacccg aatttgcaga aaagcagggc cagagagcta tcgccggcgg
      781 tatacgcagc aagcgaaaag aagaccagag cgagaatcga aaagcccgag gccaagccat
      841 tcaggccgtc gatgatgttg atggcattcg tgatgcccga aatcatgagc agcgtgaagg
      901 cggcagccag cggccacgga atctgggcaa actcaaagtc gatcaccacc gcattcagca
      961 acgcaatcgc caggacaccc gagagactga tgaccgccag gcgaatcttg ggtgagatat
     1021 tcgaaagcag gtcttcgaag aacccggcga ggaaggccgg cacggcagca atcagcagct
     1081 tgcggccaaa cggatcgtca accacgagca cgcaggcgag aaaaataccc agcccgccga
     1141 tacgtggaac gatgcgggta tgaaccttct gacgcttttc ctggtcatcg agaaacaggc
     1201 gttttttctt cgagaaccag atgattccga gatgaaagaa gtacgagatg aaaaaggcgg
     1261 ccagcagatt caacgcaagc tcactcgaag tagtggaagg tcgtaaaccc acccgttttc
     1321 aaatactcgt cgcgctggct ttctttcatg tctttggcca ccatatcctt cacaagttcg
     1381 gccaggttgg ttttcggctt ccagcccagt ttcgtcttgg ccttggtcgc atcaccaagc
     1441 aacagctcga cctcggtggg gcggaagtag cgcggatcaa cttcgacgag cgtctcgccc
     1501 gtcttcgcgt tcacgccctt ctcgtcgact ccctggccct tccaggtgat gttgataccg
     1561 acttcacgga aggccatctc gacgaactcg cgcacggtga cggtaacccc tgtcgcgatg
     1621 acgaagtctt caggcttcgg ttgttgcagc atgagataca tggcttcaac gtagtcacgg
     1681 gcatggcccc agtcgcgttt cgatgagagg tttcccagat acagtttctt ctgcaggcct
     1741 tctttgatac ggcaaaccgc acgcgtgatc ttgcgggtca caaacgtctc cccgcgcagg
     1801 gggctctcgt gattgaacag aataccgttg caggcataga gaccgtatgc ttcgcggtag
     1861 ttgacggtga tccagtaggc atacagtttt gccgcagcgt atgggctgcg cggatagaac
     1921 ggtgtcgttt ccttctgtgg cgtttcctgc accagaccgt acaactcgct ggtcgaggcc
     1981 tgatagaagc gggttttctt ctcgagcttg agaatgcgaa tcgcttcgag caagcgcaga
     2041 acgcccaatc catcggcgtt cgccgtgtat tccggcgttt cgaaactgac ctgaacatgg
     2101 ctctgcgcgg ccagattgta gatctcgtcc ggctgaacct cctgaataat acggatcagg
     2161 ttcgtcgaat cggtcatgtc accgtagtga agacggtaat cgtg
//
LOCUS       DAORHD010000882         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61647_length_2201_cov_3.265144, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000882 DAORHD010000000
VERSION     DAORHD010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61647_length_2201_cov_3.265144"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKO06_16615"
     CDS             <1..634
                     /locus_tag="PKO06_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71328.1"
                     /translation="ARLSGQSGAPADLEEHLKRVEMWEFRDRLVMDCSKGMRQRTDIA
                     RLLLRQARLIFLDEPFSGLDPCGQVMLKELLLSLKQQGIGILVNSHAAGILADVCDRV
                     CIMHRGRLLLSDRLESLLRTDRIRVRASFAEPSTAAAFLAGNTGIHAGSVPSTIEATV
                     SGQSQVTELVRRLQNSGGTLQEVTPLSLSLDQLFVQVIGSRNPDAKGGIH"
     gene            636..1409
                     /locus_tag="PKO06_16620"
     CDS             636..1409
                     /locus_tag="PKO06_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV71329.1"
                     /translation="MFTRVRLIMWDTFLDVARHKMLVIHLVFVCIALGLFNIFGHFST
                     TPALEYRMIQDVGLSIISLFGLLIALFIGSTTLRDDIHRRTVYAVLTLPMRRWELYTG
                     KFLGTLLAVIVNVLIMLGILTFLLFLKFNVVWTGFHWVALFMLFEFSVMSGMVLLFSL
                     ADSLLLCFSLSLFTVMLGNMTEHVLHLVEHAEIPLLSFFTTTLYWVIPNFGYFNIKHK
                     ILKDLAISGDFALWATGYACCYLFFLLVAGSWLFEKKDL"
     gene            1411..>2201
                     /locus_tag="PKO06_16625"
     CDS             1411..>2201
                     /locus_tag="PKO06_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71330.1"
                     /translation="MSDARKYWFHFVISLVVTAFSQYQFQHCYRRDMMASSVQQELQI
                     VQKHAAHFGDDTVFAFPDSPPGYTATASASPGTVGHSCPMCSHKMGTDTNILVSPALG
                     SHSEPLHTHERHTCHACTHDHADGCASETSSHACDHDHAAEPGHSHAHHHALDQAPPD
                     PTVSTGLVLMLRQLGFAELAANLLWIQMDADSHRGLWHRVEFALELIPVLDPTFIDAF
                     LLRAFMLDEYQSRHDEAVQILESAVKQNPNRLELWQQLGLQHLNHT"
BASE COUNT          401 a          653 c          588 g          559 t
ORIGIN      
        1 tgcccgtctc agtggacaat caggagctcc agcagacctt gaggaacatc tgaaacgcgt
       61 cgagatgtgg gagttccgcg accggctggt catggattgt tccaagggta tgcgccagcg
      121 aaccgacatc gcgcgtctcc tgttgcgtca ggcccggctg atctttctcg acgagccatt
      181 cagcggcctg gacccttgtg gtcaggtcat gctgaaagag ttgctgctgt cgttgaaaca
      241 gcagggcatc gggattctgg tgaactctca cgcggccggc attctcgcgg atgtctgtga
      301 tcgggtctgc atcatgcatc gcgggcgact gctgctcagc gatcgcctcg agtctctgct
      361 gcgcaccgat cgcatccgcg ttcgcgcctc tttcgccgag ccgtcgacag cggccgcctt
      421 tcttgccggg aacaccggga ttcatgccgg ttcagtacct tcaaccattg aggcgaccgt
      481 gagtggtcag agtcaggtca cggagctggt gcgtcgcctt cagaattcag gcggcactct
      541 gcaggaggtg acgcccctga gtctgtcgct cgaccagttg tttgtccagg ttatcggcag
      601 tcgtaaccca gatgcgaagg gggggataca ctgatatgtt cacccgagtt cgcctgatca
      661 tgtgggatac cttccttgat gtcgcccgtc ataaaatgct ggtcattcat ctggtattcg
      721 tctgtatcgc cctgggcttg ttcaacatct tcggacactt ttccacgacg cctgcgctcg
      781 agtatcgcat gatccaggat gtcggcctgt cgatcatctc gctgtttggt ttgctgatcg
      841 ccctgttcat cggttcgacg actctgcgtg acgacattca ccgccgcacc gtctatgccg
      901 tgctgacgct gcccatgcgt cgctgggagc tgtacaccgg caagttcctg ggcacgctgc
      961 tggcggtcat cgtcaacgtc ctgatcatgc ttggaatcct gacgttcctg ttgtttctca
     1021 aatttaacgt cgtctggacc gggtttcact gggttgccct gttcatgctg ttcgaattca
     1081 gcgtcatgag cggcatggtt ctcctgttct ctctcgccga ttctctgttg ctctgctttt
     1141 ctctctcgct gttcacggtc atgctcggca acatgaccga acatgtactg catctggtcg
     1201 agcacgcgga gattcctttg ctctcgttct tcaccaccac cttgtactgg gtcattccaa
     1261 attttggcta cttcaacatc aaacacaaga ttctcaaaga tctggcgatc tcgggcgact
     1321 tcgccctctg ggctaccggt tatgcctgct gctacctgtt ctttctgctc gtcgccggct
     1381 cctggctgtt tgaaaagaag gatctgtgac atgagcgacg cccgaaagta ctggtttcat
     1441 ttcgtgatat cgctcgttgt tactgcattt tcacagtatc agtttcagca ttgttatcgg
     1501 cgcgacatga tggcctccag cgtccagcag gagttacaga tcgtgcagaa gcatgcggct
     1561 catttcggtg atgacacggt cttcgcgttt cccgattctc caccggggta cacggcaacc
     1621 gcatccgcgt caccggggac cgtcggccat tcctgcccca tgtgctccca caaaatggga
     1681 acagacacga atattctggt ttcaccagcg ttggggtctc actctgagcc tctgcacacc
     1741 catgagcgac acacgtgtca tgcctgtact catgaccatg ccgatggttg tgcatcagaa
     1801 acgtcgtctc acgcctgcga tcatgaccac gcagccgagc caggtcacag ccacgcgcat
     1861 catcatgctc tcgatcaagc ccctcccgac ccgacggttt cgacgggact ggttctcatg
     1921 ctgagacaac ttgggtttgc cgagctggcg gcgaatcttc tctggatcca gatggatgcg
     1981 gactcccaca gaggattgtg gcatcgggtc gagtttgctc ttgaactgat ccccgtgctc
     2041 gaccccacgt ttatcgacgc ctttctcctg cgtgcgttca tgctcgatga gtatcagagt
     2101 cggcacgatg aagcagtgca gattctcgag agcgccgtga agcaaaaccc gaaccgactt
     2161 gaactctggc aacaactcgg gctgcagcac ctgaaccata c
//
LOCUS       DAORHD010000883         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61651_length_2201_cov_3.070829, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000883 DAORHD010000000
VERSION     DAORHD010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61651_length_2201_cov_3.070829"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKO06_16630"
     CDS             complement(<1..219)
                     /locus_tag="PKO06_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71331.1"
                     /translation="MKPLRPFLFSTQELGRSAFETVTGGREFLHLLRGVGATFSTVLL
                     AYAARAGVKAVVSEVSRMLAGIGLNVFQG"
     gene            complement(216..1175)
                     /locus_tag="PKO06_16635"
     CDS             complement(216..1175)
                     /locus_tag="PKO06_16635"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV71332.1"
                     /translation="MADSHSTKHCALGLDLGGTKIAAGLCREGQIIRKTIVPTRSDLA
                     PEEVIDAMAAACREVMSGWDGEPIRGVGVGAAGQIDAATGTVLYAPNLRWSDVPLGSS
                     LSRKLDLPVKVVNDVRAATVAEWKFGAGKGIEHFVNIFLGTGIGSGFVLNGRLLEGVT
                     NSAGEIGHICMDPEGPVCGCGKKGCLEAFSSGRGVENIVKKKLQAGERSLIAELVQGD
                     WDKVRGPIIGQAARNGDPLALEVLLQAGTVLGRALANVHSMLNPKIILLGGGFMALQE
                     FFLPACERALIEHVLPVAKRPDLLGRAEFTTDAGIIGSSALFA"
     gene            complement(1193..2149)
                     /gene="moaA"
                     /locus_tag="PKO06_16640"
     CDS             complement(1193..2149)
                     /gene="moaA"
                     /locus_tag="PKO06_16640"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNV71333.1"
                     /translation="MTSERVPFEYLRVSVTDRCNLRCVYCMPVSGVPKRGHGEILRYE
                     ELYRIVCAAVAEGVFRVRITGGEPLVRKGISGFVHMVAQIAGLRDLAMTTNAIRLAPM
                     AQELKTAGLHRVNISLDSLQPQRYEQITRGGCLSDVLAGIDAALEVGLSPVKINVVLV
                     PGVNDDEVEAFGRLAAAKPVQIRFIERMPFAVGDNHAPFITQEQLQNQLQSLFHLIPS
                     DSIVGGGPANVYRIDGGAGCLGFISPRTRPFCRSCTRLRLTSSGRLMACLDALAGVQV
                     REKSEAEIRQVIQQLGQEKKAGGKSCAGFRQTTCASLSDIGG"
BASE COUNT          456 a          728 c          601 g          416 t
ORIGIN      
        1 cccctgaaac acgttcagtc cgatccctgc gagcattcga ctgacctcgc tgaccacggc
       61 cttgactcct gctcgtgcgg cataggccag caacacggtg gaaaaagtcg cccccactcc
      121 cctcagcaga tgcaaaaact cgcggccgcc ggttaccgtc tcaaaagccg aacgtccgag
      181 ttcctgcgtc gaaaagagga atggtcgaag gggtttcatg cgaacagggc actgctgccg
      241 ataatgccgg catcggtggt gaattcagcc cgcccgagca gatcggggcg tttggctacg
      301 ggcagcacgt gctcgatcaa cgcccgctca caggctggca gaaagaattc ctgcaatgcc
      361 atgaagccgc cgcccaacaa gatgatcttc ggattcagca tggaatgcac gttcgccagg
      421 gcgcgcccga gaacggtgcc cgcctgcagc agcacttcga gggcgagcgg atcgccgttc
      481 cgcgccgcct ggccgataat cgggccacgc actttgtccc agtcgccctg cacgagttcc
      541 gcgatcaaac ttcgttctcc ggcctgcagt ttcttcttca cgatgttctc aacgccccgc
      601 cccgaagaaa atgcttcgag acagcccttc ttgccacaac cgcacaccgg accttccgga
      661 tccatgcaga tgtgaccgat ctcgccggcg ctgttggtca cgccttcgag aagccgtccg
      721 ttgaggacaa agcctgaacc aatgccggta ccgagaaaga tgttgacgaa gtgctcgatg
      781 cccttgcccg ctccgaattt ccattcggcc accgtcgcgg cacgcacatc gttgaccacc
      841 ttcaccggca gatcgagctt ccgcgacagc gagcttccca gcggcacatc agaccagcgg
      901 agattcggcg catacaggac ggttcccgtg gcggcatcga tctgccctgc ggctccgacg
      961 ccgaccccac ggatcggttc cccgtcccat ccggacatca cctcgcgaca ggcagccgcc
     1021 atcgcgtcaa tcacctcttc cggcgccaga tccgatctgg tcggaacaat ggttttgcga
     1081 atgatctgtc cttcgcggca aagaccagcg gcaatcttcg ttccgcccag atcgagcccc
     1141 agcgcacagt gctttgtcga atgcgaatct gccataccca ctccttcgat gtttacccgc
     1201 cgatatcgga aagtgaggcg cacgtggtct gtcgaaaccc ggcacagctc tttcccccag
     1261 cctttttttc ctgacccagt tgttgaatga cctgtcgaat ctcggcttct gatttttcgc
     1321 gcacctgaac cccggccaat gcatcgagac aagccatgag ccggccgctc gacgtcagac
     1381 gaagacgcgt gcaggaccgg cagaagggcc gagttcgtgg actgatgaac cccaggcaac
     1441 cggccccacc gtcgatacgg tagacattcg ccggtccacc gcccacgatc gaatcagacg
     1501 ggatcagatg gaataggctc tgtaactgat tctgcaactg ttcctgggtg atgaacggtg
     1561 cgtgattgtc gccaacagca aagggcatgc gctcgatgaa tcggatctgt accggcttcg
     1621 ccgcggcaag acgcccgaat gcctcgactt catcgtcgtt gactcccggg acaaggacca
     1681 cattgatttt gaccggggaa aggccaacct cgagcgcggc gtctatgcca gccagaacgt
     1741 ctgacaggca ccctccgcga gtgatctgtt cataccgctg cggttgcagc gagtcgagac
     1801 tgatgttcac ccggtgcagt ccggcggtct tcagttcctg ggccatgggt gccagacgaa
     1861 tcgcattcgt ggtcatcgcc aggtcacgca gcccggcaat ctgcgccacc atgtgcacaa
     1921 acccgctgat gcctttccgc accagaggct caccaccggt gatgcgcacc cggaacacgc
     1981 cttcagcgac agcggcgcac acgatccgat agagttcttc gtatcggaga atctctccgt
     2041 gcccccgctt cggaacccca cttacgggca tgcagtagac acagcgcagg ttacatcggt
     2101 cggtcaccga tacgcgcagg tattcaaacg gaacgcgttc gctcgtcatg atcgggcata
     2161 ctagggccgg acaggaatgg tgtcaaggag tgatcgcccc c
//
LOCUS       DAORHD010000884         2198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61772_length_2198_cov_3.292114, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000884 DAORHD010000000
VERSION     DAORHD010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61772_length_2198_cov_3.292114"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PKO06_16645"
     CDS             <1..410
                     /locus_tag="PKO06_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71334.1"
                     /translation="SGRILVVDDEASVRRMSELFLRNIGFEVATAENGREALNLFAAT
                     PGGFALVLLDMLMPEMDGRTCFFELKKLNPQVKIILSSGFSHLQDLQELQAAGLRTFL
                     RKPFHLVELSRAIARELQGSGGTSEPEVSDKLV"
     gene            complement(359..1096)
                     /locus_tag="PKO06_16650"
     CDS             complement(359..1096)
                     /locus_tag="PKO06_16650"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNV71335.1"
                     /translation="MSSTFTALLGSLRRETLCAFLRSLTQPCYESSLLHAAFPDFDII
                     RASPVELYRHHFALFHVLYDLQDQFRDEGSHLHVHFMRTSLVPFPPDGCCRHFEGSLG
                     RFCGVPVTASATICDTHAGLFGEKQLDALSDRYFYADARNFEALAEEQAEAFLQGTWE
                     VLGSHDRLRKAFDTLGLAPTCERKVIRNRFAALARECHPDAGAGSAARFCEINAAYRL
                     VLRLLDAFEPHQTSLSDTSGSDVPPEP"
     gene            1273..>2198
                     /locus_tag="PKO06_16655"
     CDS             1273..>2198
                     /locus_tag="PKO06_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV71336.1"
                     /translation="MKTRNILIPQHGKVFRHICALCLFLLSALVVIPATGETIRFGFN
                     NTILSGVTQADAESALALWADFVAKEEGVEGESKVTGYKTVEDMLVDLRGEALDVAML
                     TAFEYLAIKDRVQLEVISVGVIQEQIGIPVVIAVHRTSPFQSLKDLQGKELLCKSMYV
                     EHIGNYWLDGVLSANGLLPTSRFFSRRRTMDKVSKAVLPVFFNKADACLVRQCALDTM
                     IELNPQLARDLRIIASSPPMLNGLCAIRPGMNATHREILIRRIPTMHQHVKGKQILTL
                     LKLDDATSYKPEMLRAAELIYQESLKLRAGPG"
BASE COUNT          488 a          625 c          644 g          441 t
ORIGIN      
        1 ggagcgggcg cattctcgtc gtcgacgacg aggcttcggt tcggcgaatg tccgagttgt
       61 ttctgcgtaa catcggcttc gaggtggcca ccgccgagaa tggtcgcgaa gctctgaatc
      121 tcttcgcggc aacgcccggc gggttcgccc tggtgttgct cgatatgctc atgccggaaa
      181 tggacggacg cacctgcttt ttcgagttga agaagttgaa cccgcaggta aagatcattc
      241 tttcgtcagg gttctcacac cttcaggatc tgcaggaact gcaagccgcc gggctccgca
      301 ccttcctgcg caaaccgttt cacctcgtcg aactcagtcg cgccatcgct cgcgagcttc
      361 agggttcagg cggaacgtcc gagccggagg tatcagacaa gctggtttga tggggctcaa
      421 aagcatcgag taatcgcagc accaggcgat aagccgcgtt gatttcgcag aatcgagccg
      481 ccgaccctgc ccccgcatcg ggatggcact cgcgcgccag agcggcaaag cgattgcgaa
      541 tcaccttgcg ctcacaggtc ggggccaggc cgagcgtgtc aaatgctttt cgcaggcgat
      601 cgtgcgaccc gagcacttcc caggtgccct gcagaaaagc ctcagcctgc tcttcggcga
      661 gagcctcgaa gttgcgcgca tcggcataaa agtaccggtc ggaaagcgca tcgagctgct
      721 tttcaccgaa taacccggca tgcgtatcac agatcgtggc agatgcggtc accggcacgc
      781 cgcagaatcg tccgagcgag ccttcgaagt ggcggcaaca accgtcgggc ggaaaaggaa
      841 ccagcgacgt gcgcatgaag tgcacgtgca aatggcttcc ctcgtcgcgg aactgatcct
      901 gcaggtcgta caagacgtga aacaacgcaa agtgatgacg atacagttca accggtgagg
      961 ctcgaatgat gtcaaaatca ggaaacgccg catgcagcag gctgctctcg tagcagggct
     1021 gtgtcagcga acgcagaaac gcgcacagcg tttcgcgacg caggctgcca agcagggccg
     1081 taaaggtgct cgacatgcgg ggttccgggc ctcacttcac acgaaatttg cgacttgtgt
     1141 cacgaccacg cgcgatcgat ttgatcgtat cactcgagcc tcatggcggc aacagccact
     1201 ccttgcacgg gttcagcaga aatgggcgct gttcgaaacg tgtgttatac tgaatacggg
     1261 tgggaacttt tgatgaaaac aagaaacatt cttattccgc aacacggaaa ggtattccgg
     1321 cacatttgcg ccctgtgcct gtttctgttg tctgctctgg tggtgattcc agcaaccgga
     1381 gaaaccatcc gattcggttt taacaacacg attctgtcag gagtcactca ggcggacgcg
     1441 gaaagcgctc tggcgctgtg ggctgacttc gtggccaaag aagagggagt tgagggagag
     1501 tcgaaagtca ccgggtacaa gaccgtcgag gacatgctcg tggatctgcg gggcgaagct
     1561 ctggacgtcg ccatgctgac ggctttcgag tatcttgcga tcaaagaccg ggttcagctc
     1621 gaggtcatct cggttggagt gatccaggag cagatcggca ttcccgtcgt gatagccgtt
     1681 catcgcacga gtcccttcca gtcgctcaag gatctgcagg gcaaggaatt gctgtgcaaa
     1741 tcgatgtatg tcgagcacat cggcaactac tggctcgatg gcgtcttgtc ggcgaacggt
     1801 cttctgccga catcgcgatt cttctcgagg cgccgcacga tggacaaagt ttcaaaagcg
     1861 gtcttgccgg tattcttcaa caaagccgac gcctgtctgg tgcgccagtg cgcgctcgac
     1921 accatgattg agttgaaccc gcaactggca cgggatctcc gcatcatcgc ctcgtcaccg
     1981 cccatgctca acggtctctg tgccattcgc cccggcatga atgccacgca tcgagagatc
     2041 ctgattcgcc gcattccgac gatgcatcag cacgtcaagg gaaaacaaat tctgacgctg
     2101 ctcaagctcg acgacgcaac cagctacaag ccagagatgc ttcgtgccgc ggagctgatc
     2161 tatcaggaga gcctgaagct cagagccggg ccaggaga
//
LOCUS       DAORHD010000885         2197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_61832_length_2197_cov_3.295051, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000885 DAORHD010000000
VERSION     DAORHD010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61832_length_2197_cov_3.295051"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKO06_16660"
     CDS             <1..688
                     /locus_tag="PKO06_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV71337.1"
                     /translation="LRTLYKQIPDTRCDNCGSCCHSVSIFSLEYHLIMRDLLTRMNPG
                     QIRQTLNGVLHLDQRLVQKQGESRLRCAFRVSETRACVVHESRPFCCRLFGQRFENTP
                     PECDHVVPLLPGASLTTSFVEDLQVKVMNNSEAHTPYEDLSPITFFPFEFWVFRYALG
                     PEKALQLYRSVLVPASTALSRFWHEERRRHAEGCHPEEHNSGQDHAGRRPRFSPRLDG
                     SEHIPDARKH"
     gene            complement(707..1237)
                     /locus_tag="PKO06_16665"
     CDS             complement(707..1237)
                     /locus_tag="PKO06_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71338.1"
                     /translation="MLTSGLGRQDSRFERRTIWLTRFEWLGVVLILFSLCFTASELDM
                     RPTGFTILRDAGTLAFAESLRDPEQSLKEAGYPREPTDVERARDLFPPDGTVTISFQG
                     RYLPMEESIKAAYRQIGTLVFGNDEPLVFTQCVALSCGWLVLGQHGRPAELGRDRVFN
                     LLIDSRRRLIWLYKTP"
     gene            1409..>2197
                     /locus_tag="PKO06_16670"
     CDS             1409..>2197
                     /locus_tag="PKO06_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV71339.1"
                     /translation="MFRQIRFQLLTLIVGVVLLAAVFIGGFVLYRIHTNLTEDAITAQ
                     RHLSNALKQGLAMKFEEIRLHLQHVAEHPLLLHPDPTRCRSLLSEFLVQNPLFFSALL
                     YDRNGTVLSAIYRNHSPIADRIVGRNLLRAGASYQRLIEAFKTVVATNQPVISDAVSS
                     VRQGPLVVMKFPVRSLDQPNQVTRVLSLGIHLNGAVLQHTLTRYAPPDGFLALIDRKG
                     EIIARAGSGMPTTLSGILLEPWPEESAPQSAWTTLNNRSFLVFAR"
BASE COUNT          451 a          698 c          576 g          472 t
ORIGIN      
        1 gctgcgcacc ctgtacaagc agattccaga tacgaggtgc gacaactgcg gttcctgctg
       61 ccattcggtc tcgatcttct cactcgagta tcatctgatc atgcgcgacc tgctgacacg
      121 catgaaccca ggccagatca ggcagacgct gaatggggtt ctacatctcg atcagcggct
      181 ggtgcagaag cagggggaaa gccgactgcg ctgcgctttt cgcgtatcag agacccgcgc
      241 ctgcgtcgtg catgagtcgc gccccttctg ctgccgtctg ttcggacaac gattcgagaa
      301 cactcccccc gaatgcgatc acgtcgtgcc gctgcttcct ggagcctcgc tgacgacttc
      361 attcgtcgag gaccttcaag tcaaggtgat gaacaattcc gaagcgcaca ccccctatga
      421 agatctcagc ccgatcacgt ttttcccgtt cgagttctgg gtcttccgct atgcgctggg
      481 tcccgagaaa gcgctgcagc tctaccgctc cgttctggtt cccgcgtcaa ccgcgctgag
      541 tcggttctgg catgaagaac gccgccgcca tgccgaaggc tgccaccccg aggaacacaa
      601 ctcaggacaa gaccatgcag gccgcaggcc acggttttct ccgcgcctcg atggttcaga
      661 gcacattcct gacgcacgca aacactgatc ggaagagttc caatggttat ggagttttgt
      721 acaaccaaat cagacgacgg cgcgagtcga tcagcaggtt gaagactcgg tcccgcccca
      781 actccgccgg gcgaccatgt tgtccgagta ccagccatcc gcaggaaaga gccacgcact
      841 gagtgaatac cagcggttcg tcatttccaa acaccaacgt gccgatctga cggtatgcag
      901 ctttgatact ttcttccatg ggcaggtaac gcccctgaaa cgagatcgtc acggttccat
      961 ccggcggaaa caagtcacga gcgcgctcga catctgtcgg ttcacgcgga tacccggcct
     1021 ctttcagaga ctgctccggg tcgcgaagcg attcggcaaa agccagggtt cccgcgtcac
     1081 gcaggatggt gaatccggtc ggcctcatgt ccagttcgct cgcggtaaaa cagagagaga
     1141 agaggatcag caccaccccg agccactcga agcgcgtgag ccagatggtt cttctctcga
     1201 agcgggaatc ctgcctgccc agacccgagg tcaacattca gcactcctgt cgtgcctgat
     1261 tcaaagcggc accagtttga cgagtacctt tttcgtccag cacaaacggt gatcaaaagc
     1321 ccttgtcagc aacagattat cacaccgccc ttcccaaatg cactggtctg cagtagtctg
     1381 tgacatcctc tctccaagga cgaccatcat gttccgccag attcgattcc agcttctcac
     1441 gctgattgtc ggtgtcgtgc tgctggcagc cgtattcatc ggcggcttcg tgctgtatcg
     1501 cattcatacg aacctgaccg aagatgcgat caccgcgcaa cgccacctga gcaacgcgct
     1561 gaaacagggg ttggccatga agttcgagga gattcggctg catctgcagc acgtggccga
     1621 acatcccttg ctgctgcacc cagatcccac gcgttgccgt tcgcttttgt ccgagtttct
     1681 cgtgcaaaat cctctgtttt tcagcgccct tctttatgat cgaaatggta ctgtattgtc
     1741 agccatctat cgtaatcact ctccgatcgc cgaccgcatc gttggtcgaa acctgctgcg
     1801 ggccggcgca agttatcagc ggctgatcga agccttcaag accgtggttg ccacgaatca
     1861 gcccgtgatc agtgatgccg tgtcgtccgt acgacagggg cctctggtgg tcatgaagtt
     1921 tcccgttcgc tcgctggacc aaccgaacca ggtcacccgc gttctcagcc tgggcattca
     1981 tctcaacggc gccgtgctgc aacacacgct cacccgctat gctccgcccg atggttttct
     2041 ggccctgatc gaccggaaag gcgagatcat cgcccgtgcc ggttcgggca tgcccaccac
     2101 cctcagcggt attctgctgg aaccctggcc tgaagagtcg gccccacagt cggcctggac
     2161 caccctgaac aatcggtcat ttctcgtgtt tgcgcgc
//
LOCUS       DAORHD010000886         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62031_length_2192_cov_4.140384, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000886 DAORHD010000000
VERSION     DAORHD010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62031_length_2192_cov_4.140384"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2192
                     /locus_tag="PKO06_16675"
     CDS             <1..>2192
                     /locus_tag="PKO06_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71340.1"
                     /translation="NVVASPTVVADTGGNLYNTRVLSTGDLSGRWLVRIENAAGTVIY
                     MQTTFDVNTPAYLVTELSVPDSLITGQAFSISHTVTNAGQSDAVGVVPTLPAIFGGGT
                     ILSLTGPSPSAWEIPGGESRTFTYNAIAGNAGRFWVRANAQGIIQGSAQTVTSASAGS
                     NISTIRTELLLSELVVASATTVNRGQSDLPLMGVLINSGTADLVVASISPRFLKGASD
                     VSANYSSTLISPSLPATIPGIAFPPWWNTAYGYRQKLRITSDNVTYAASCTAFIKLDL
                     NALIAANKLQTNYNDWRVVRWDDLVATWTELDRDRIDTEQTAFAIRAAILPNGRDESY
                     YVYYGNPAAVAPPSDKRRCYLWYEDFETPVYGAAWNRLANGALAFTGWTGADGGQDGI
                     YATRGVMSRPPGAQSIAFRQIFFNNVATTTANFARFFRVTDTTNYPRLRISFWRWYDD
                     ACDWQNPSPTNFDWARLRFLDNLGIWNLIIFYPSNGPDDDTWRFEEYDLSNMVVGPTT
                     QFSWDGNFSRGAADPNDRIYFDDICLWMRTPTVLAQGEETQPVTTLGATFSIDVGVTA
                     PTGSTTLDLAATATSALASGVYADLTANTPDEWLVATITLTTWADAGGTIPKSEFARG
                     QTVYVRGTGFTPGGGNVTINWIDGYPTGTVRNTTILPEGFGAISGSAIPAAAGDFGTW
                     RLAATQGAVTATGSFRLLERPAFIPFFHLTPATSTVGDILTASLTVHA"
BASE COUNT          432 a          691 c          607 g          462 t
ORIGIN      
        1 caatgtcgtc gcgtctccca ctgtcgtggc cgacaccggc gggaatctgt acaataccag
       61 agtcttgtcg accggcgatc tcagcggaag atggctggtc cgaatcgaga atgccgccgg
      121 cacagtgatc tacatgcaga cgacgttcga cgtgaacaca ccggcgtatc tcgtcactga
      181 gttgtccgtg cccgacagtc tcatcaccgg gcaagctttc tccatttcgc acaccgtaac
      241 caacgccgga caatcggacg cggtcggggt ggtgccgacc ctgccagcca ttttcggcgg
      301 tggcacgatc ttgtcgctga cagggccttc ccccagcgcc tgggagattc ctggcggcga
      361 gagtcgcacc ttcacctaca acgctatcgc cgggaatgct gggcgtttct gggttcgcgc
      421 gaacgcgcag ggcattattc aggggtccgc tcaaaccgtc acctcagctt cggctggttc
      481 aaacatatcg accatcagaa ccgaactgct tctatcggag ctggtcgtcg cttctgccac
      541 gaccgtcaat cgtggccaga gtgaccttcc cctgatggga gtcctgatta atagcggaac
      601 ggcggatctc gtcgtcgctt cgatatcacc acgatttctc aagggagcga gcgacgtcag
      661 tgccaactac tcttccaccc tgatctcgcc gtctctgcct gcgacgattc ccggtattgc
      721 ctttccgccc tggtggaaca ccgcctacgg ctatcggcag aaactgcgca ttaccagcga
      781 caacgtgacc tatgcagcat cctgtactgc gttcatcaag ctcgatctca atgcgctgat
      841 tgccgcaaac aagcttcaga cgaactacaa cgattggcgg gtggtgcgat gggacgacct
      901 cgttgccacc tggaccgaac ttgaccgcga tcgtatcgac acggagcaga cagcgttcgc
      961 catccgagcg gccattcttc cgaatggtcg tgacgagagt tactatgtgt attacggaaa
     1021 tccggcggcg gttgccccgc cctcagacaa acgtcgatgc tatctctggt atgaggattt
     1081 cgagacgccg gtctatggtg ctgcttggaa taggctggca aacggggctc tcgctttcac
     1141 aggatggacg ggagctgatg gtggccagga cggcatctat gccaccaggg gtgtcatgtc
     1201 gcggcctccc ggagcacagt cgattgcatt tcggcaaatc tttttcaaca atgttgccac
     1261 caccacagcg aactttgcac gcttctttcg agtgaccgat accaccaact acccacgtct
     1321 gcgcatttcc ttctggcgct ggtatgatga cgcttgtgac tggcaaaacc cttcaccaac
     1381 aaattttgac tgggcacgtc tgcgatttct cgacaatctc ggtatctgga atctcatcat
     1441 cttctacccg agtaacggcc ctgacgatga tacctggcgt tttgaagagt acgatctttc
     1501 caacatggtc gtcggcccaa caacgcagtt ttcatgggat gggaattttt cccgtggcgc
     1561 tgccgacccc aatgaccgca tctacttcga cgacatttgc ctctggatgc gaacacccac
     1621 ggtgctcgcg caaggagagg aaacccagcc ggtcacgacg ctgggagcga cgttcagtat
     1681 cgacgtcgga gtaaccgcgc cgaccggatc gacgacgctc gatctggcgg ctaccgccac
     1741 cagtgctctg gcgagcggcg tctacgcgga tctgaccgcg aacacacccg atgagtggct
     1801 ggtcgccacc atcaccctga cgacctgggc agacgccggc gggaccatcc cgaagtccga
     1861 gtttgcccgc gggcaaaccg tctacgtacg cggcaccggc ttcaccccag gcggtggcaa
     1921 tgtcaccatc aactggatcg acgggtatcc gacgggcacc gttcgcaaca ccaccattct
     1981 gcctgaagga ttcggggcga tttccggcag tgccattccg gcggccgctg gcgacttcgg
     2041 tacctggcgg ctggcggcca cgcagggggc ggttaccgcc accggctcat tccgcctgct
     2101 cgaacgtccg gcgttcatac ccttcttcca cctgactccg gcgacttcga cggtcgggga
     2161 cattctcacg gcgagtctga ccgttcatgc cg
//
LOCUS       DAORHD010000887         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62036_length_2192_cov_3.822181, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000887 DAORHD010000000
VERSION     DAORHD010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62036_length_2192_cov_3.822181"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1247)
                     /locus_tag="PKO06_16680"
     CDS             complement(150..1247)
                     /locus_tag="PKO06_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71341.1"
                     /translation="MADISPVPEPTIGAAMPAEPAPAPASTTDSADPPAAVPVPPSAP
                     LTPARDPRRLAQALIIWGVAFLAAVVVGPLLPAWPASVAYFLASLALIGLAVNLVIGF
                     QHLVLHRTTYLLLGALGLAATLQIAQPHCERTRGLNLVCGLPGELLTLTGRLSAVPFG
                     QDGINTARNSLFGELSGFVEPAFAPESWWRVLILGLAQLLIAAGVGLWIGFGIDEIGH
                     LIPIALVAGLADIWSVSAGATAVIITSPHIHYFLLRFPMLGGDKPLMPFLIGLTDFLF
                     FAIFYTAAVRFALPQRRTLNLLLASFVVTVALALFFQVGLPVLPFMAVLFVAGNWHHL
                     RVKHDDLVKVGLFLLVCGAVAFGLTILIHGK"
     gene            complement(1307..>2192)
                     /locus_tag="PKO06_16685"
     CDS             complement(1307..>2192)
                     /locus_tag="PKO06_16685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71342.1"
                     /translation="YHETGRSLDALRQYELVLKQEPQHLNALNNLGLVYSNIGERERA
                     IDLFNRVIGLEPGNVKAHINLGNAFLNGKQWAEAEASYKMAIALDDKDLSPKINLGVV
                     QFEQGNFDQARQQWESILKSDPDNLRVLSVMASGYLEKKKFDEAISIFKRMVKLMPNN
                     GGMANTLGYLLAEQNKELAFAKELIERALELDPPNRAAYLDSLAWVLYRKGQFEKARD
                     LQERALKIFRLIHEPITSEVYLHMGKIYERLKKNNEAREAFEEALKANSDPDAVRDAE
                     EGLARLTPKSTGETGKIE"
BASE COUNT          440 a          636 c          702 g          414 t
ORIGIN      
        1 ccgaagcgaa catcacacag cgtgcgcacg atctgcggct gtttgagacg actgaagtcc
       61 tgcatcaatt catcgaaaaa cagaaaatag ttgcccagtc cgtgcatctt gtgaaattca
      121 aacattccct tgctcctcga ttgagatggt tactttccgt gtatcaggat agtcaggccg
      181 aaggcgacgg ctccgcacac caacagaaac agaccgactt tgaccaggtc atcatgcttt
      241 acccgcagat gatgccagtt tccggccacg aacagcacgg ccatgaacgg cagtaccggt
      301 aagcctacct gaaagaacag ggccagcgca acggtgacga cgaatgaggc cagcagcagg
      361 ttgagcgtgc gtcgttgcgg cagggcgaag cgaacggcgg cggtgtaaaa gatcgcgaag
      421 aagagaaagt cggtcagccc gatcagaaat ggcatcagcg gtttgtcgcc cccgagcatg
      481 ggaaaccgga gcaggaaata gtgaatgtgc ggcgaggtga tgatgaccgc ggttgcccct
      541 gccgagacac tccagatatc ggccagaccg gccaccaggg cgatcggaat cagatgcccg
      601 atctcgtcga tgccgaagcc gatccagagc ccgacgccgg cggcgatcaa cagctgcgcc
      661 agacccagta tcagcacgcg ccaccacgat tcgggggcga acgccggttc gacaaaccct
      721 gacagttcgc cgaacagcga attgcgggcc gtattgatgc cgtcctggcc aaagggaacg
      781 gcggaaagcc gaccggtcag ggtgagcagc tcgccgggca ggccgcagac aaggttgaga
      841 cctcgggtgc gctcacagtg cggctgggca atctgcagcg tcgcggccag accgagagcg
      901 cccagcagca gatacgtggt gcgatgcagc accaggtgct gaaagccgat gaccagattc
      961 acggccagac cgatcagggc cagagaggcg agaaagtagg cgaccgaagc gggccaggca
     1021 ggcagcagcg gaccaaccac gacggcggcg aggaaggcaa ccccccagat gatgagcgcc
     1081 tgcgccaacc gccgcgggtc gcgtgccggg gtcaggggcg cagagggcgg gacggggacc
     1141 gcggcaggtg ggtcagcaga atcggtggtc gatgccgggg cgggagccgg ttcggcgggc
     1201 atcgcggcac cgatcgtcgg ttccgggacg ggagaaatgt ctgccatggc tgactccctt
     1261 gataccctgc gcggcgtttc cccgtgggcc gggtggcgcc ggtgttctac tcgatctttc
     1321 ccgtttctcc cgtgcttttc ggcgtcagtc gggcaagtcc ctcttccgcg tcacgcacgg
     1381 cgtccgggtc ggaattggct ttcagggcct cttcaaaagc ttcccgcgcc tcattgttct
     1441 tcttcagtcg ttcgtagatc ttgcccatgt gcaggtagac ctcgctggtg atcggctcat
     1501 ggatcaggcg gaaaatcttg agtgctcgtt cctgcagatc gcgggctttt tcgaactgcc
     1561 ccttgcgata cagcacccag gcgagcgaat cgagatacgc ggcccgattg ggcggatcga
     1621 gctcgagcgc ccgttcgatc agttctttcg cgaaggcgag ctctttgttc tgttcggcga
     1681 gcaggtagcc gagggtgttc gccatgccgc cattgttggg catgagcttg accatgcgct
     1741 tgaagatgct gatcgcttca tcgaacttct tcttctcgag gtagccgctg gccatgaccg
     1801 agagaacccg caggttgtcg ggatccgact tcagaatgct ttcccactgc tggcgggcct
     1861 ggtcgaagtt gccctgctcg aactgcacga ccccgaggtt gatcttcggg ctcagatcct
     1921 tgtcgtcgag cgcgatggcc atcttgtagc tggcttctgc ctcggcccac tgtttgccgt
     1981 tcaagaaggc gttgccaagg ttgatatggg ccttcacgtt gccgggttcc aggccgatca
     2041 cgcggttgaa caggtcgatg gcccgctcgc gctcgccgat attcgaatac accagaccca
     2101 ggttgttgag cgcgttcagg tgctgtggtt cctgcttgag taccagttcg tattggcgca
     2161 gagcatcgag ggaacggcct gtctcgtggt ag
//
LOCUS       DAORHD010000888         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62081_length_2191_cov_4.304775, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000888 DAORHD010000000
VERSION     DAORHD010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62081_length_2191_cov_4.304775"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /locus_tag="PKO06_16690"
     CDS             <1..1453
                     /locus_tag="PKO06_16690"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV71343.1"
                     /translation="VFIYRGMLDFCRSDDELATILAHELAHSERRHSLKQLRGNAAFA
                     LLLQAAVKNKKDQQTWGAVVGALTSLAFSREHEDEADDLGQTRMFQAGFDPSAQVLVW
                     EKFVQKFGKGPGGIMQYLSTHPPSQDRVANARKNLTKFQVPERKDFTLSFNVTAETEE
                     NLIQNGSFETDVGKRGFPDAWEKREGQVAPDGATSYTGKASLSVAATSNVRPARVVSE
                     LIPVNPAGSYELSGFGRAQSAANKVSIGAEIYDKNKRLRGYIWPVLSNGQFGTDWQTF
                     SGTFAPGKEPGKSLPPDTAFIRILLQSGPFSQGNIWFDHLVLKPAGSKPSDNLIPDGN
                     FEFSGADGQLRGVLATPGAVSQDKEFCKVGYASLKMTGNGGETAMMLDPLMVEQFKPG
                     QELRLAMHYCGSAEFKFRAMLELVDAAGKPLARRLVEKELTAKPNLWQAEGIRVRFEL
                     QEAEKGVARALVVKVVGTIPAGSHLWLDGFTLR"
BASE COUNT          474 a          614 c          630 g          473 t
ORIGIN      
        1 agtgttcatc taccggggca tgctcgattt ctgccgctct gatgacgaac tggcgacgat
       61 tctggcccat gagctggcgc actcagaacg ccggcactcg ctgaaacagc tgcgtggcaa
      121 cgcggcattt gccctgttgc tgcaggcggc ggtaaagaac aagaaagacc agcaaacctg
      181 gggcgccgtc gttggcgctc ttaccagtct ggccttttca cgcgaacacg aagacgaagc
      241 tgatgatctg ggccagacgc gcatgttcca ggcggggttc gatccttctg cgcaggtgct
      301 ggtctgggaa aaattcgtgc agaagttcgg caaggggccg ggcggcatca tgcagtatct
      361 cagcacccac ccgccgtctc aagatcgcgt cgcgaacgct cgcaagaacc tgacgaagtt
      421 tcaggtgccc gaacgcaaag atttcaccct ctccttcaac gtgacggcag aaaccgaaga
      481 aaatctgatc cagaacggca gcttcgagac cgatgtcggc aaacgcggct tccccgacgc
      541 ctgggaaaag cgagaaggcc aggtggcccc cgatggcgcc acaagttata ccgggaaggc
      601 ctctctctcc gtcgcggcga cctcgaatgt gcgtcccgcg cgcgtggttt cggaactgat
      661 tccggtgaac cccgcgggtt catacgagct ttccgggttc gggcgggcac agtcggcggc
      721 gaacaaagtg agcatcggcg cagaaatcta cgacaagaac aaacgcctgc gcgggtacat
      781 ctggcccgtt ctgtcgaacg gacagttcgg cactgactgg cagacgtttt ctggcacctt
      841 tgcccccgga aaagagcctg gaaagagctt gccccccgat acggccttta tcagaatcct
      901 gttgcagagc ggtccgttct cacagggcaa catctggttc gatcatctgg tgctgaaacc
      961 ggctggcagc aagccctcgg ataacctgat tccggacggc aacttcgagt tttccggcgc
     1021 tgacggtcag ctgcggggag tattggcgac tccgggcgcg gtctcgcaag acaaggagtt
     1081 ctgcaaagtc ggctatgcct cgctgaagat gaccggaaac ggcggcgaaa ccgccatgat
     1141 gctggatccg ctgatggtgg aacagttcaa accaggtcag gaactgcgtc tggccatgca
     1201 ctactgcggc tcggccgaat tcaaattccg cgccatgctc gagctggtcg atgcagccgg
     1261 aaagcccctg gctcgccgtc tggtcgagaa agaacttacc gccaagccga atctgtggca
     1321 ggccgaaggc atacgggtgc gattcgaact gcaggaggcg gaaaagggtg tggctcgcgc
     1381 ccttgtcgtg aaggtggtcg gaacgattcc ggctggttca cacctctggt tggacggttt
     1441 tacgctgcga taggaagaaa gcgaacacaa gccagacagt cggctggatg tcgattgtct
     1501 ggctgcgttg tctctgctgt actgtcgtgt ggggctcacg aagttggtca cgatcttcac
     1561 gcggaaacta ccgggtcacc gaaaatccag aatcgaatgt cgttccccga gcatctgcgc
     1621 tgattgaaca cgagcctgcc gattgccccc ttcagagcga acgatgaaat cgtcttatca
     1681 ttattctgac gataatcaat ttgtgatacc aaatcgtttc gatatggttt acatatttat
     1741 taattgtatt atcattactc tgaccgcctg ttagtatcat ttgtctagta gcacggcaac
     1801 gtctcttttt attctcctgt aagcaatggc gacgcgagtc gtccgcagaa gaaaacctgt
     1861 cagcgagagg cgactcgtga gcatcggcgg gccgttcgaa ccccgtgctc ctttcggtcg
     1921 cgaggctgaa tcgatgaccg ctgcgagttt cgaacagtga gccctggtgg ctgattccct
     1981 ggtaagtatt gaggccattg aagctgactc ttcctgatag ctcctctgtt gatcctgccg
     2041 ctcatgcgca gcacccagcg ttttctccac atgacggcct ctgtacctgc gctacgggta
     2101 gattactctg tctgaacgcc ctacctgagc gaggttttgc gacaataatg tcgctataaa
     2161 atgcgttcaa cgtgcatctg caagcccagg c
//
LOCUS       DAORHD010000889         2189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62178_length_2189_cov_3.443768, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000889 DAORHD010000000
VERSION     DAORHD010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62178_length_2189_cov_3.443768"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..69)
                     /locus_tag="PKO06_16695"
     tRNA            complement(1..69)
                     /locus_tag="PKO06_16695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Leu,seq:caa)
     gene            complement(633..>2189)
                     /locus_tag="PKO06_16700"
     CDS             complement(633..>2189)
                     /locus_tag="PKO06_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV71344.1"
                     /translation="YPEGHAPLVRFMTVPVFKGDAIVAVVGVANKSSDYDESDVLQLT
                     LLMDSVWKIVDSKKNEEALRESQERHRVILQTAMDGFWFTDIQGKLLEVNDTYCQMSG
                     YSRAELLTMSISDIDAQELPEQTATHLTQVIEQGQHRFESRHRRKDGTMFDVEISVQY
                     RAEGIGGLVAFLRDISDRKRAEQERVKLESQLLQSQKMESVGRLAGGVAHDFNNMLGV
                     ILGYTELALEGIGPDHPLHASLNQIQKAATHSAELTSQLLAFARKQTATPQVLDLNDT
                     VIGMFKMLQRLIGEHIQLVWRPGANLWPIRIDPAQVNQILANLCVNARDAISATGKIS
                     IETANSAFDADSCASHKDLIPGEYVRLSVSDTGIGMDREILSHLFEPFFTTKGVGKGT
                     GLGLATVYGIVKQNGGFINVYSEPGNGSTFTMYFPRHGEKPPRKTRDAVAASEAAQGA
                     TILLVEDELTLLDLAKTILSRQGFTVLAAKTPGEALQVASCRPCVSQTPDIPGERSPC
                     RCAPARSGNS"
BASE COUNT          444 a          630 c          621 g          494 t
ORIGIN      
        1 ggtcggagtc gaaccgacac gcctgtgagg gcgcgggatt ttgagtcccg tgcgtctgcc
       61 agtttcgcca ctccggcaat agagccagtg tagcagattc gggtgggaaa ttcaattcgt
      121 acgtgcagag aatttcgagg gtgtgccaca aaatgtgaca cattttagac gaggtgttca
      181 tggtcatggg ctccagagac acgttgacgc gcggggattt ttttcacttt cggcggcgga
      241 ttccgcggaa gctgcaacag atcttcggtt tgaggaggca ggcggctcaa gcgttttctt
      301 cagaacgaaa gtgctattat tggtggaaag tgttaccgtg tacgaacgaa acgttcataa
      361 cgagttgaac atgcaacgaa catcccgtat cgaactcccc tgctcatcaa agccacgtgg
      421 cacacgtgca gatcgaacac attcattcgt gttacctgct ttcctgcaga aaacggtgcg
      481 gcgacgagac gggtttcgcc actctctgta cgcacttggc ctgttcctgc tgctgtgtct
      541 cgtgtcggct tcggcggcgc aggttcagaa aacggtttcg acgccggatg ttcagcttca
      601 gttgcggcat ttcgaagcac ccaccatgta cgtcatgaat tccccgaccg ggcaggcgcg
      661 catcggcaag ggcttctttc gcccggtata tcgggggttt ggctcacgca aggccgacaa
      721 ctcgccacct ggagcgcctc accgggagtc ttggcggcaa gcaccgtaaa gccctgccga
      781 gacagaatgg tctttgccag gtcgagcagc gtcagttcat cttccaccag caggatcgtg
      841 gcgccctgag cagcttctga tgccgccacg gcatcacgag ttttgcgcgg cggtttctcg
      901 ccatgccgcg gaaagtacat ggtaaaggtc gaaccgttcc cgggttcgct gtagacgttg
      961 ataaacccgc cgttctgttt gacgatgccg tagacagtgg ccagacccag accggtgcct
     1021 ttcccgaccc ccttggtagt gaagaacggc tcgaacaggt gagacaggat ctctcgatcc
     1081 atgccaatgc cggtgtcact gacggagagc cgcacatact ctcccgggat cagatccttg
     1141 tgactggcgc aggaatcggc gtcaaaggcg ctgtttgccg tttcgatact gatctttccg
     1201 gtcgcggaaa tggcatcgcg ggcgttgacg cacaggttcg ccagaatctg gttcacctga
     1261 gccggatcaa tgcgtatcgg ccacaggttc gccccgggcc gccacaccaa ctgaatatgc
     1321 tcgccgatca accgctgcag catcttgaac attccgatga cggtgtcgtt cagatcgagc
     1381 acctgcgggg tcgccgtctg tttgcgggcg aaggccagca gctgcgatgt gagttcagcg
     1441 gaatgggtgg ccgccttctg gatctggttc aggctggcat gaagcggatg atcgggaccg
     1501 attccttcca gtgccagctc cgtatacccc agaatgacgc ccagcatgtt gttaaagtca
     1561 tgcgctacac cgccagcaag gcggcccacg gattccatct tctgggactg tagcagctga
     1621 ctctcgagct tcactcgttc ctgttcggct cgcttgcggt cagagatatc gcggaggaaa
     1681 gcgaccagac caccgattcc ttcggcgcga tactgtacgc tgatttccac atcgaacatc
     1741 gtcccatcct tgcgccgatg ccgtgactcg aaccgatgct gtccctgttc gatcacctgc
     1801 gtcagatggg tggccgtctg ctcaggcaat tcctgggcat cgatgtcgga gatgctcatg
     1861 gtcagcagtt cagcgcgaga gtatccactc atctgacagt aggtgtcatt gacctccagc
     1921 agttttccct ggatatccgt gaaccagaag ccatccatcg cggtctgcag aatgacccga
     1981 tgacgttcct ggctctcgcg caacgcttcc tcgttttttt tgctgtcgac gattttccag
     2041 acggaatcca tgagcaacgt gagctgcagc acatcggact catcataatc gctggatttg
     2101 ttcgccaccc cgaccaccgc gacgatggca tcgcccttga acaccggtac ggtcataaac
     2161 cggacgagag gcgcatgccc ttcgggata
//
LOCUS       DAORHD010000890         2184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62400_length_2184_cov_3.397370, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000890 DAORHD010000000
VERSION     DAORHD010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62400_length_2184_cov_3.397370"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="PKO06_16705"
     CDS             complement(<1..1089)
                     /locus_tag="PKO06_16705"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV71345.1"
                     /translation="MKTRVFPARFAICIVLVLIALLTATASWAADQRPGVEEVFDRMK
                     TGAADPNAQLRFLPENVNAWYARWYILDNAKKSIDLTYFIVEPDIFGMSMLGMLMKKA
                     REGIQIRIMMDARGTKGLVRKFIGQDVLQELAKFKNVQIRVFNPVGQKLIALFDDLRN
                     IMASNHDKIILVDGEYLITGGRNISKNYFVDPRDLATVYRDTDILIKSPIVAAQARRA
                     FEEEFTSGGNFKIDKDLFGNVDDMHRHLDIAYQAMRRYLLGNGLYKLPEDNNLSKDTR
                     KLVEKYTTELAQYKQLPGYASFRLFQGERAYPTRILDKHSRNGNRNEITPNLIALMDA
                     CKEEILIQNPYVVLTPESHAALKRANDRG"
     gene            complement(1245..>2184)
                     /locus_tag="PKO06_16710"
     CDS             complement(1245..>2184)
                     /locus_tag="PKO06_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71346.1"
                     /translation="DTGKQYRSIKLSEVVLKGQKALGVGLERATQSIKQSFSVKNVAM
                     TAGTSIAINLASQVINGEKPSFKKAFQAVASLQFVGNVVGSSLGAAAGHMVAPLIQTF
                     VPIPIVGTLAGALLPTFASIAGGQFGGNLGAGVGFKQAVKNLDWVAISGQAVGSTLGA
                     MLGAMIPVPVLGPMIGGMLGGILGEKVFKGIAKLFGFGKNKAATPVSTPIAPPAANPF
                     AAYQQPAATAAPIQSADRKKGSDPANERLAASIDRIPYEQMHPNLRKVKDAYEKAYRD
                     YVQALGSGNSSSAQSALTTFRAEKDRYHRALGAYLK"
BASE COUNT          446 a          588 c          652 g          498 t
ORIGIN      
        1 cccgcgatcg ttcgcccgct tcagagctgc gtgtgattcg ggagtcaata cgacataggg
       61 gttttgaatc agaatctctt ccttacaggc atccatcagc gcaatcaggt tcggcgtgat
      121 ctcgttccgg ttcccgttgc gggaatgctt gtcgagaatg cgggtcgggt acgcgcgttc
      181 gccctgaaac aggcggaacg aggcataacc aggcagctgc ttatactgcg cgagctcggt
      241 cgtgtatttc tcgaccagct tgcgagtgtc tttggaaagg ttgttgtctt cgggcaactt
      301 gtacaagcca ttgccgagca gataccggcg catggcctga taggcgatat ccagatgccg
      361 gtgcatatcg tccacgtttc cgaacagatc tttgtcgatc ttgaagttgc cgcccgaggt
      421 gaattcttcc tcgaaggcgc gccgggcctg tgccgcgacg atcggactct tgatgagaat
      481 gtcggtgtcg cgatacaccg tcgccaggtc gcgcgggtcg acgaaatagt tcttcgaaat
      541 gttgcggccg ccggtgatca gatactcgcc atcgacgaga atgatcttgt catggttgct
      601 ggccatgatg ttgcgcaggt cgtcgaacag ggcgatcagc ttctggccga ctgggttgaa
      661 aacgcgaatc tggacattct tgaacttggc cagttcctgc agaacatcct ggccgatgaa
      721 cttgcgcacg agacctttgg tgccgcgggc atccatcatg atgcgaatct gaatgccttc
      781 gcgggctttc ttcatcagca tgcccagcat gctcatgccg aagatgtcag gctcgacgat
      841 gaaataggtc aggtcgatcg atttcttggc attatcgaga atgtaccagc gggcatacca
      901 tgcattgacg ttttcaggca ggaagcgaag ttgggcgttg ggatcggcag caccggtctt
      961 catgcgatcg aagacttctt cgacgccggg gcgctggtcg gcggcccagg aggctgttgc
     1021 ggtcagcagt gcgatcagga cgagcacgat acaaatggcg aaccgggctg ggaatacacg
     1081 tgttttcatg tactgtctcc ttgatcctga gaaacttcga tgttccatga tacctcagat
     1141 tcagaggttt gaaaacggaa acctgatacc gtacggaaca gggcctggaa aaaaaacaga
     1201 agcaggaatc tgtttcctgc ttctgtggca atcagtcgag agggctactt cagataggct
     1261 cccagagcgc gatgatagcg atctttttcg gcccggaagg ttgtgagagc cgattgtgcg
     1321 gaagatgagt tgcctgaccc gagggcttgc acatagtcgc gatacgcctt ctcgtaggcg
     1381 tctttgacct tgcgcagatt tggatgcatc tgttcatacg gaatgcgatc gatcgatgcc
     1441 gccaggcgtt cgttggcagg atctgaacct ttcttgcggt cggcggactg aatgggtgcc
     1501 gcggttgccg ccggctgctg atatgcggcg aacgggttcg ctgccggagg ggcaatcggt
     1561 gtcgagacgg gcgtcgctgc tttgtttttc ccaaatccga agagtttggc gatccctttg
     1621 aagaccttct cgccaagaat gccgcccagc atacccccga tcatgggccc cagcactggt
     1681 accggaatca tggctccgag catggccccc agggttgacc cgactgcctg tccggagatg
     1741 gcgacccagt cgaggttctt caccgcctgc ttgaaaccga cacccgcgcc cagattgccg
     1801 ccgaactgac cgccggcgat cgacgcgaag gtcggcaaga gagcgccagc cagcgttccg
     1861 acgatgggaa tcgggacgaa cgtctgaatc aggggggcca ccatatgccc ggcggccgct
     1921 ccgagagatg aaccaaccac atttccgacg aactgcagcg aggcgaccgc ctgaaacgct
     1981 ttcttgaagc ttggtttctc gccgttgatg acctgactgg ccagattgat ggcgatcgag
     2041 gtgccggccg tcatggcgac attcttgaca ctgaatgact gtttgatcga ctgggtggcg
     2101 cgctcgagac cgacacccag agctttctga cccttcaaca ccacttctga cagtttgatg
     2161 ctgcgatact gttttccggt atcg
//
LOCUS       DAORHD010000891         2182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62517_length_2182_cov_2.147626, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000891 DAORHD010000000
VERSION     DAORHD010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62517_length_2182_cov_2.147626"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2182)
                     /locus_tag="PKO06_16715"
     CDS             complement(<1..>2182)
                     /locus_tag="PKO06_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71347.1"
                     /translation="TALMRQLEALKNINQYGEQAGTLINQLTDANNRYDDEVKKINSE
                     THRSEADKRKALKAAADLRDEEIKRIRIATDAALTMADADREMALESDDHKRRRLEIM
                     KLAQTEIQKITDDEAKATALLDSQLKNKTITQDQYETQRTALSKKYADARFGIEAKMQ
                     ADLMTLEELRIAEEAKNEQDRLNALAEQKRKEMELQRNLQEAELEAARKWDDELTGRE
                     EDDGYDLEALRRRWELEFNIKSDLERKLFQIEVEYLDELAKLEAAYANDSEEYKRGLK
                     FLDDIKAKKIKEAKEPVREEYDLEALRRRWETEFNIKSELKRALFQIEVEYIDEAAKL
                     ERAYAKDTAEYKAGLKKLEEIKEKKIEDARRTASMELIDTISGYYSSAMTMVSKDVVG
                     GLQGLMSQVANQTQTAWLGWASFGVGVIKDFFDLFGSKSESALDRMKKQLQSIMDEMD
                     RVSDKTGIGLAEKIFGADSSKYSEEALKSANDAVKVWREANPLLAWMDDEQIYEFRKK
                     LKNADFTKINWGDYTATSQNWETIGPGRMGWKEKNLNIFDTKHIDPRTLTANDVTALV
                     GQIRELFENMYGEYADEMLASFGLTGTDQQIWGKWVGGVKNRLTEVNNMLIESAGKAA
                     EALQLLDDAKVRDALATYKEQLADIDHKLKTGKLTDEEAIKAQLEVYDQMLLQIESLD
                     ESTEKEAARNKLLEERYALEQNLLALQDKEKNGLSEILATEEGRA"
BASE COUNT          356 a          627 c          555 g          644 t
ORIGIN      
        1 tggcccggcc ttcctctgtt gcaagaatct ccgagagtcc gtttttctct ttgtcctgga
       61 gtgccaagag attctgttca agcgcatacc gttcttcgag gagcttgttc cgggcggctt
      121 ccttctcggt tgattcgtcc aggctctcaa tctggaggag catctgatcg tatacctcaa
      181 gctgggcctt gatggcttcc tcgtcggtga gcttcccggt tttcagtttg tggtcaatgt
      241 cggcaagctg ttccttgtac gtggccagtg cgtcccggac ttttgcatca tcgaggagct
      301 ggagcgcttc ggcagccttc ccggcggact cgatcagcat gttgttgacc tcggtgagcc
      361 ggtttttcac accgccgacc catttgcccc agatttgctg gtcagtaccg gtcaagccaa
      421 aacttgccag catttcgtcc gcgtattccc catacatgtt ttcaaaaagt tcacggatct
      481 ggccgacaag ggcagtcacg tcgtttgctg tgagtgttcg tggatcgata tgcttcgtgt
      541 cgaagatatt gaggttcttt tccttccaac ccatccgccc ggggccaata gtttcccagt
      601 tttgggatgt ggctgtgtaa tctccccagt tgatctttgt aaaatcagcg ttcttgagtt
      661 tcttccggaa ctcgtaaatc tgctcgtcgt ccatccacgc aaggagcggg tttgcttcgc
      721 gccacacctt aacggcatca tttgcgcttt tgagtgcttc ctcgctgtac ttggacgagt
      781 cagcgccgaa aatcttttca gccaggccaa tccctgtctt gtcgctcact cggtccattt
      841 cgtccatgat ggactggagc tgctttttca tgcggtcaag cgctgactcc gacttgctcc
      901 caaaaaggtc gaagaaatcc ttgatcaccc cgaccccgaa ggaggcccat ccgagccacg
      961 cggtttgagt ctgattcgca acctggctca tgagcccctg tagaccaccc acaacgtctt
     1021 tcgacaccat agtcatagcc gaggaatagt atcctgaaat cgtgtcaatc aattccatcg
     1081 aggcggtgcg tcttgcatcc tcgatcttct tttccttgat ctcttcgagc tttttcaaac
     1141 cggccttgta ctctgcggtg tctttcgcat aggcccgctc cagctttgca gcttcgtcga
     1201 tgtattccac ttcgatctgg aagagcgcgc gtttcaattc cgatttgatg ttgaactcag
     1261 tctcccaccg acggcggagt gcttcaaggt cgtactcttc ccggactggc tccttggctt
     1321 cttttatctt cttggccttg atgtcatcga ggaacttcag gccgcgcttg tattcctctg
     1381 agtcgtttgc ataggccgct tccagttttg ccagctcgtc gagatactcg acttcgatct
     1441 ggaagagttt tcgttcaaga tcactcttga tattgaactc caactcccac cggcggcgga
     1501 gtgcttcgag gtcatacccg tcgtcctctt cccgcccggt cagctcatca tcccacttgc
     1561 gagcggcttc cagttcggct tcttgcagat ttcgttggag ctccatctct ttgcgcttct
     1621 gctccgcaag agcattgaga cggtcctgct cgttcttggc ttcctcggca atcctcaatt
     1681 cttcgagggt catgaggtcg gcttgcatct ttgcttcaat cccgaatcgc gcgtcggcgt
     1741 acttcttgga aagagctgtc cgttgcgttt cgtattggtc ctgtgtgatc gtcttgtttt
     1801 tgagctggct gtcgaggaga gcggttgcct tcgcttcgtc gtccgtgatt ttctggatct
     1861 cggtctgggc cagcttcatg atctccagcc gacgacgctt gtggtcatcg gattcgagag
     1921 ccatttcccg gtcagcgtcg gccatggtca gagcggcatc ggtcgcaatc cggatccgct
     1981 tgatctcttc atcccggagg tcagccgcgg ccttgagtgc cttccgcttg tcagcctcgg
     2041 accggtgcgt ctccgagttg attttcttca cttcgtcatc gtaccggttg ttggcatcag
     2101 tgagctggtt gatgagagtc ccggcctgtt ccccgtactg gttgatgttc ttcaaggctt
     2161 cgagctgacg catgagggcc gt
//
LOCUS       DAORHD010000892         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62584_length_2180_cov_3.488941, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000892 DAORHD010000000
VERSION     DAORHD010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62584_length_2180_cov_3.488941"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PKO06_16720"
     CDS             complement(<1..1453)
                     /locus_tag="PKO06_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71348.1"
                     /translation="MKRAFTLVLALVFTLSTIVPSFAAGAFESTSSGVRYVVRDANGK
                     IVQSFKPTYDAATGKYIVKDQENRVIAQQSLAPEAGMGQIKIVAPVSGDSHSVTSTDF
                     LGNRVPVLRELPANDPRYVQLKSYIESDAGLRKVVSMQVQARNYKLDTLQQEISQGTR
                     DPKLAAWLVNDLKKPVYLEVGDSGSIYHDPRGFIMAEQAANGQVVNRDNAYANRIVVP
                     PNSEVFTGSMKDSAAASVMAHEMGHMIMDQVYETPNYPRSSYAGPHSKDSITDPGFAY
                     HEGWAEAIETLSNKDRLYSSNNWRVASQRNVAENKYIFKNQGVLEGPNDGLLKSGAQM
                     LSTEGVNATLFYKMLTDNRIQAPYSKVLQVFEQSKPQNYNEFLTKYIEKFPEDRSQVI
                     KGFLESTKYATVDSTATARYKALHDAEIAYKNAGNSAPEVQAQLEQQYKQQLNEYNQW
                     KEQTYKQTVVDGKIDRALDQNAQVAMSYSDDTGK"
     gene            complement(1689..1979)
                     /locus_tag="PKO06_16725"
     CDS             complement(1689..1979)
                     /locus_tag="PKO06_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71349.1"
                     /translation="MELLIIVCSSTMKDAVEEVFDQAGISSYTRVPEVVGSGKGGGTR
                     LNDEIWPGVNTLYLIAPTLEQAKTVKDWVREYRREDVREGLKVFSLAAQEIM"
BASE COUNT          441 a          576 c          618 g          545 t
ORIGIN      
        1 gttttccggt atcgtctgag taggacatgg cgacctgggc gttctgatca agggcgcggt
       61 cgatctttcc gtcaacaacc gtctgtttgt aggtctgctc tttccattga ttgtactcgt
      121 tcagttgttg cttatactgc tgttcgagct gagcctggac ttctggagca ctgtttcctg
      181 cgttcttgta tgcgatctcg gcgtcatgca gggctttgta acgagctgta gctgtggaat
      241 ccacggtcgc atacttggta ctttcaagga aacccttgat gacctgactg cgatcttcgg
      301 gaaatttctc gatgtacttc gtcagaaact cgttgtaatt ctgaggcttg ctctgttcga
      361 acacctgcag aaccttcgaa tagggggcct gaatgcggtt gtcggtcagc atcttgtaga
      421 agagcgttgc attcacgccc tcggtgctga gcatctgcgc tccgctcttc aaaagaccgt
      481 catttggtcc ttcgagtaca ccctgattct tgaaaatgta cttgttctct gcaacattcc
      541 gctgactggc gacacgccag ttgttcgacg agtacagtcg gtccttgttg ctgagggtct
      601 caatggcttc agcccaacct tcgtgatacg cgaatccggg gtcggtgatc gagtctttgc
      661 tgtgaggtcc ggcatagctt gagcgcgggt agttgggcgt ttcgtagacc tgatccatga
      721 tcatgtgacc catctcgtgg gccatgaccg atgccgccgc cgaatctttc atgctaccag
      781 tgaacacttc gctgttcggg ggaacgacga tgcggttggc atacgcgttg tcacgattca
      841 ccacctggcc attggcggcc tgctcggcca tgatgaagcc gcgtggatca tggtagatcg
      901 agcctgagtc tccgacctca agatagacgg gcttcttcag atcgttgacc agccaggcgg
      961 cgagtttggg atcgcgagtt ccctgggaaa tttcctgctg cagggtgtcg agcttgtagt
     1021 tgcgggcttg aacctgcatc gaaaccactt ttcgcaggcc ggcatcgctt tcgatgtagc
     1081 tcttcagctg tacgtaacgc gggtcattgg cggggagttc acgtagcacc ggaacccggt
     1141 tgccgagaaa gtcggttgag gtgacgctgt gggaatcacc actgacgggg gcaacgatct
     1201 tgatctgccc catgccggct tccggagcga gcgattgttg ggcgatcacc cggttttcct
     1261 ggtcttttac gatgtattta ccggtggcgg catcgtaagt gggtttgaac gattgtacga
     1321 tctttccgtt ggcatcccga acgacgtagc gcactccgct ggaggttgat tcgaaggcgc
     1381 ctgccgcgaa tgagggaacg atcgtactga gggtgaaaac cagcgccaga accagggtaa
     1441 atgcccgttt catcagtgta cctccgctcg gaatgccgat tgttccaatt ataccaaatg
     1501 gtcgccacac cccacaagac atggaggccg cgtgtgttaa cggacagtga taatgtcatg
     1561 agttaacggg acagtgaaaa tgtcagggta tggaaacggt aaaaaaatcc cgaggcgatg
     1621 aacggggtgt tccgcctgac gcagaggggc gagggcttcg atgcgatttt tcgaacgctt
     1681 gtgccgtctt acatgatttc ctgtgcggcg agcgagaaca ccttcagccc ctcgcgcacg
     1741 tcttcgcgac gatactcgcg cacccagtct ttcactgttt tcgcctgttc gagtgtgggg
     1801 gcgatgagat acagggtgtt cacacccggc cagatctcat cgttcaggcg agtgccaccc
     1861 ccttttccgg aaccgaccac ttccgggacg cgcgtatacg aagaaatgcc cgcctgatcg
     1921 aagacctctt cgaccgcgtc tttcattgtg ctgctgcaaa cgatgatcaa cagttccata
     1981 ccaactcctc ttttcgccac agccagatca agcctgtctg tgggccatca atgagagtga
     2041 tgcgctcgtt cagaatcagc tgactccgag tgttttgagc aggcgtcgac tgaatagaga
     2101 atacaggacc ggcatcaaca ccagagtcat gatcgtcgaa atgaccaggc cgccgatgac
     2161 gctcagggcg agcggaaccc
//
LOCUS       DAORHD010000893         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62599_length_2180_cov_2.813647, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000893 DAORHD010000000
VERSION     DAORHD010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62599_length_2180_cov_2.813647"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKO06_16730"
     CDS             <1..570
                     /locus_tag="PKO06_16730"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV71350.1"
                     /translation="LAIRSYELAAICYKARRANHRILGYTYLSGAWAARDAREQSKDP
                     ERRKEFLDFELAFLREAVNFLTITNKATSIEDTYMPDGTKIQKENMPQSRIFEVMYIL
                     AGAHRMLEELDDSNKYLEQIIYGGASAQGIILWFVQQARDMRQSDQVGPIEVGDPIEA
                     ELEPGEGEEDEDGESDENEPEEGKKGWFF"
     gene            694..1452
                     /locus_tag="PKO06_16735"
     CDS             694..1452
                     /locus_tag="PKO06_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71351.1"
                     /translation="MTVFEPRDHIDSRNQQRGSALVLVTVICLIVAVAAGGWWYWSRL
                     VQQKKAKGLEDELQTRLNEGNAALEQGKMDEAYKAFLAGRRIFDDPIFLAWRTPSTGP
                     ASPASELTDKSEVDQLLTTISLFKAYDEAFQLKPAAEWAGKAEEHAKSLSGPAADDTR
                     KRVATARSVAQLIELYRQKKYETVLKDLLTVEKNSQSGDRDFFIMEVRLLIACGKGLK
                     QPDIVAQARKLLFFLQFEARLEDSRLDQLWSLLG"
     gene            1611..>2180
                     /locus_tag="PKO06_16740"
     CDS             1611..>2180
                     /locus_tag="PKO06_16740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71352.1"
                     /translation="MLDNDLCVEYELDACLIATASCDLPVCSDEHLESAWLWLADVHD
                     LLAGIRRLPPQHSPSVSPWLIAAHDVPDLLLMRHRPGLALSLLRKAWKQAPADIEASP
                     ELLAVVVGLLEPFAPVLGWSLRQHFGTPLRTLEETRQFLARTVPNLRLIRLQIDRGAR
                     HTVVVHTQQWQQNPLQALAQQEAWAARLIA"
BASE COUNT          482 a          651 c          604 g          443 t
ORIGIN      
        1 ctggcgatcc gatcgtatga gttggccgcc atctgctaca aggcccggcg tgcgaatcac
       61 cgcattctcg gatacaccta tctttccggc gcctgggcgg cacgcgatgc ccgtgaacag
      121 tcaaaagacc ccgagcgacg caaagagttc ctcgattttg agctcgcatt cctgcgcgag
      181 gcggtgaact ttctgaccat caccaacaag gcgacgagta tcgaagatac ctacatgccc
      241 gatggcacca agattcagaa agaaaacatg ccccagtcgc gcattttcga ggtcatgtac
      301 attctggcag gggcgcatcg catgctcgaa gagctcgatg acagcaacaa gtatcttgaa
      361 cagatcattt acggtggggc ttcggctcag ggcatcattc tctggttcgt gcagcaggcc
      421 cgagatatgc gccaatccga tcaggtcggc cccatcgaag tcggtgaccc cattgaagcc
      481 gagctcgaac ccggcgaagg cgaagaagac gaagacggtg aaagcgacga gaatgaaccc
      541 gaggaaggaa agaagggttg gttcttctga tcacccgcga gtgcctatct cggccagccc
      601 ccgtctgctc cggcccgggg gctcgttttt gtaccgggaa gaccacgcca gaacccagtc
      661 tgagtcacga atacagtgga cggaaagaag ctcatgaccg tgtttgaacc tcgagatcat
      721 atcgattcac gaaaccagca gcgcggctct gcgcttgtcc tcgtgaccgt gatctgtctc
      781 attgtcgcag tcgctgctgg aggctggtgg tactggtccc ggctggtaca acagaagaaa
      841 gccaaggggc tcgaagacga attgcagacc cgtctgaacg aaggcaacgc cgcactcgaa
      901 cagggtaaga tggacgaggc ctataaagcc tttctggccg gtcgtcgcat cttcgatgat
      961 cctatttttc tggcgtggcg aaccccttcg acaggcccgg cctctccagc atcggagctg
     1021 accgacaaga gtgaagtcga ccagctgctg acgacgatca gcctgttcaa agcctacgac
     1081 gaagcctttc aactgaagcc tgccgctgag tgggccggga aagcggaaga gcatgccaaa
     1141 tccctgagcg ggccagcggc ggatgacacc cgaaagcggg tcgccacggc ccgcagcgtg
     1201 gcacagctga tcgagctgta ccgtcagaag aagtatgaaa cggtgctcaa agacctgctc
     1261 accgtcgaaa aaaactcgca atcaggcgat cgtgacttct tcatcatgga agtccgacta
     1321 ttgatcgctt gcggcaaagg tctgaagcaa cccgacatcg tcgctcaagc ccgcaaactg
     1381 ttgttttttc tgcagttcga agcacgtctg gaagactcgc gcctggatca gctgtggagc
     1441 ctgctcggat aatcacggag cggtataatc cacaccggct cgttcgtttg taccggcaag
     1501 ctcacactca ccttacgtct caatcaacgt tttccgagtt attgagatct cgtgctcacg
     1561 cactctggaa cgacggaaaa tcgaccacgg ctttcgattg tccttttcca atgcttgaca
     1621 acgatttgtg cgttgaatac gaactggacg cttgtctgat cgctactgcc agttgcgatc
     1681 ttccggtgtg ttctgacgag catctcgaat cagcctggtt gtggctggcc gacgtacatg
     1741 acctgctcgc cggtattcgg cgactgccgc cacagcattc cccctccgtt tctccctggc
     1801 tgatcgctgc ccacgatgtc cccgatctgc tgttgatgcg ccatcgaccc gggctcgccc
     1861 tctccctgct gcgaaaggcc tggaaacagg caccagcgga catcgaagcc agccccgagt
     1921 tactggccgt ggtcgttggt ctcctcgagc cgttcgctcc ggttctgggc tggagtctgc
     1981 gacagcactt cggcactcca cttcgaaccc tggaagagac ccggcagttt ctcgcaagaa
     2041 ccgttcccaa cctgcgactc atcaggctgc agatcgaccg tggcgcgcgc catacggttg
     2101 tggtacatac acagcagtgg cagcaaaatc cgctgcaggc tcttgcccag caggaagcct
     2161 gggcggcacg cctgatcgcc
//
LOCUS       DAORHD010000894         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62631_length_2179_cov_3.036723, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000894 DAORHD010000000
VERSION     DAORHD010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62631_length_2179_cov_3.036723"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKO06_16745"
     CDS             <1..400
                     /locus_tag="PKO06_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71353.1"
                     /translation="YWGTLCKPCGGDGYVAISGEPMACAGCQGKGKDYWGTVCTGCSG
                     TGWANTTTNPAWGGHMTSTACGFCGGKGKDYWGTVCDKCGGDGYVTGPSDHKQCASCA
                     GKGKDYWGSVCAGCAGNGFARFVEMPISGK"
     gene            568..>2179
                     /locus_tag="PKO06_16750"
     CDS             568..>2179
                     /locus_tag="PKO06_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71354.1"
                     /translation="MVSATGQAADRGGGIIRHATVVAWLSIPFLLVAMAYAWYWDFRA
                     RECRGQDEKQLQGMLTAWGSEAVPEYRVRRILEAVDQRIFGPGMTRQRQKRLLQGLHR
                     RFPGWFRFTVLDADGVPSSELCDLPQPKAAWQRVNAAYRQFLAGDPEPLKNIKTTVRS
                     LLGSFVTTGRRMSGTMLRADADRRRRWLYAGLPQADGWFIAHVAAQKDWFSLPLQDRI
                     DRWNERQSPVRLYLLQGVPPRPETPDLASAPIAQLYDRLDPLVWDGDSLWGLQRVGPR
                     LSLVAVRHNTWPRGQRREAATIAWLILAFWLSTIVVGTRPRVTAAALAISIKWRLAAS
                     VGFATALPFAIIACTTLAYVREHGHKLEGDLYFRMEGALRDLDDLAPFLIERQNALLL
                     KQATELSWPVKRPDIWDAHLKKLERIAPWDLAKIYGPGGKTLLTNASHSSLIWSPEDL
                     GIFGQAAKHCLRRMQVQDPDASAEEKTQGLSFLENTMANIAGQTGKMAEYIAGEERLL
                     LMFLPLMEQGRVRSLLLLIWNRWRMERDF"
BASE COUNT          406 a          605 c          697 g          471 t
ORIGIN      
        1 ctattgggga acgctctgca agccgtgcgg tggcgatggg tatgtcgcga tcagcggtga
       61 accgatggcc tgtgccggct gccagggcaa aggcaaggat tactggggaa ccgtctgcac
      121 gggctgttcc ggcaccggct gggctaacac cacaacgaat cctgcctggg gcggtcacat
      181 gacttcgacg gcatgcgggt tctgcggagg caagggcaag gactattggg gtaccgtttg
      241 tgacaaatgc ggcggcgacg gctatgtaac cggtccctct gatcacaaac agtgcgcctc
      301 ctgcgcgggc aaaggcaagg attattgggg ctccgtgtgt gccggttgcg caggcaacgg
      361 tttcgcccgc ttcgtcgaaa tgccgatcag cggcaagtaa acaaaaccga tagagctccc
      421 cggcttgcgc tcagacgccg ccggggagtt tttctgtacg cccggggatc tgttctctgc
      481 tgttccacct cattcaaaca atctcaccgg cggccgactg gttgcatcgt caggttggca
      541 acgctacact gaacggcgag gtgtctcatg gtgagcgcga ccggtcaggc agcggaccgt
      601 ggcgggggaa tcattcgtca cgcaacggtg gtggcgtggt tgtccattcc gtttctgctg
      661 gtggccatgg cctatgcctg gtactgggac tttcgcgcac gcgagtgtcg cggtcaggat
      721 gagaaacagt tgcagggcat gcttaccgcc tggggatctg aggccgtgcc cgagtatcgg
      781 gtgcggcgta ttctcgaggc ggttgaccag cgaattttcg ggcctggcat gaccagacag
      841 cgacagaaac gtctgttgca ggggctgcat cgtcggtttc cggggtggtt ccgcttcacg
      901 gttcttgatg ccgacggggt tcccagttcg gaactgtgcg atctgccgca gccaaaggcg
      961 gcgtggcaac gcgtcaacgc ggcgtatcgc cagtttctgg ccggagaccc ggagccactc
     1021 aagaacatca aaaccacggt gcgctcgttg ctcggttcgt ttgtgacgac cgggcggcgg
     1081 atgagtggga cgatgctgcg cgccgatgca gatcgccgtc gacgctggct ctatgccggt
     1141 ttaccccagg ctgacggttg gttcatcgcc cacgttgccg cacagaaaga ctggttttca
     1201 ttgccgctgc aagaccgtat tgatcgctgg aacgaacgtc agtcaccggt acgactgtat
     1261 ttactgcagg gcgtgccacc tcggccggaa acccctgacc tggcttcagc ccccattgct
     1321 cagctgtatg atcgtctcga tccgcttgtc tgggatgggg attcgctgtg ggggttgcag
     1381 agagtcgggc ctcgattgtc gctggttgct gtacggcaca acacctggcc ccgtgggcag
     1441 aggcgcgaag ccgcaacgat cgcatggctg attcttgcgt tctggctttc gacgatcgtt
     1501 gtcggcaccc gcccacgcgt gacggcggca gcgctggcga tttcgatcaa atggcgtttg
     1561 gcggcttcgg ttggttttgc caccgcactg ccgtttgcca tcattgcatg tacaacgctc
     1621 gcgtatgtgc gtgaacacgg gcacaaactc gaaggtgacc tgtactttcg tatggaaggc
     1681 gcgctgcgtg atctggatga tctggcaccg tttctgatcg aacgtcagaa tgcgctgtta
     1741 ttgaagcagg ctaccgaact ctcctggccg gtcaagcgcc cggacatctg ggacgcacac
     1801 ttgaaaaaac tcgaacgtat tgccccctgg gatcttgcca agatctacgg accgggtgga
     1861 aagacgttgc tgaccaacgc gtcgcactcc tcactgatct ggtcaccgga agacctcggg
     1921 attttcggac aggcggccaa gcattgtctg cggcgcatgc aggttcaaga tccagatgcc
     1981 agcgctgaag agaaaacgca gggtctttcc tttctcgaga acacaatggc gaatatcgcc
     2041 ggccagaccg ggaaaatggc agagtacatc gctggtgagg aacgcctgtt actgatgttc
     2101 ctgcccctga tggaacaggg tcgggttcgt tcactgctgc tgctgatctg gaatcgctgg
     2161 cgcatggaac gggattttc
//
LOCUS       DAORHD010000895         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62738_length_2176_cov_3.847713, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000895 DAORHD010000000
VERSION     DAORHD010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62738_length_2176_cov_3.847713"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="PKO06_16755"
     CDS             <1..1823
                     /locus_tag="PKO06_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015109514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HNV71355.1"
                     /translation="RSIYTLWRQVLEYFDAYLIGLTATPAKHTYGFFEQNLVMSYTHE
                     EAVAEGVNVDFEVYRISTKITEEGGRINRVEDVQIGLRNRQTRKVKWQKPDEDITYTA
                     ADLDRAVVAQDQIRKIVQTFRDRVKTEIFPGRKEVPKTLVFAKDDSHAEDIVNIIRQE
                     FAQGDEFCQKITYKTTGKSAKDLIQEFRNRYQPRIAVTVDMIATGTDIKPIEIVIFMR
                     SVKSRVMFEQMKGRGVRTIKPDELQAVTPDSRSKTHFVIIDCIGVTETPLADTHPLER
                     SKSVSFKSLLEHVAMGGTDPEMVSSLAARLARLDKQCGPKEREKIAEAAPGVSLPKIC
                     ADIVHALDPDRQEEKARETFAIPADQEPTDAQIEAARKALVKEAVKPLATNPKLRTCL
                     EALKTELEQIIDTVTPDEITQAGFAKEAKEKAKSLVQSFEEFLKEHQREIDALQFFYS
                     QPYQRRLRWADIKKLHEAIAAPPRQWTEDKLWAAYELIEQGKVRGARAERRLTDLVSL
                     IRFALHQEQELVPFAERIRERFAAWLKNQESQGRTFTADQHRWLEMIRDHVAQSYEIS
                     IEDFGYTPFDREGGLGKAAQIFGMTTLKPLIDELHEALVA"
     gene            1820..>2176
                     /locus_tag="PKO06_16760"
     CDS             1820..>2176
                     /locus_tag="PKO06_16760"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNV71356.1"
                     /translation="MKSKEIASWLEVKLEDVIEILDHRREPVNSTERESRIGNVPYYG
                     ATGQVGWIDDYLFNEDLILLGEDGAPFLDSKKSVAYQIHGKSWINNHAHVLRAIAGCP
                     NAFFAHQLNTVDYRPFV"
BASE COUNT          535 a          654 c          614 g          373 t
ORIGIN      
        1 accggtcgat ctacacgctc tggcgccagg tgctcgaata cttcgacgcc tatctgatcg
       61 gcctcaccgc tacgcctgcc aagcacacct acgggttctt cgaacagaat ctggtgatga
      121 gctacacgca cgaagaggcg gtcgccgagg gcgtcaacgt cgacttcgaa gtttaccgca
      181 tcagcacgaa gatcaccgaa gagggcggca gaatcaaccg ggtcgaagat gtgcagatcg
      241 gcttgcgcaa ccgccagacc cgcaaggtga agtggcagaa gcccgacgag gatatcacct
      301 acaccgccgc cgacctcgac cgcgccgtcg tggcacagga tcagatccgt aagatcgtcc
      361 agacgttccg cgaccgggtg aagaccgaga tttttccggg tcggaaagag gtgcccaaga
      421 cgctggtctt cgccaaagac gactcgcacg ccgaagatat cgtcaatatc atccgtcagg
      481 agttcgcgca gggcgacgag ttctgtcaga agatcaccta caagaccacc ggaaagagcg
      541 ccaaagacct gattcaggag tttcgcaacc ggtaccagcc gcgcatcgcc gtgacggtcg
      601 acatgatcgc caccggcacc gacatcaagc cgatcgagat cgtgatcttc atgcgctcgg
      661 tgaagagccg cgtgatgttc gagcagatga aggggcgcgg cgtgcgcacg atcaagcccg
      721 acgaactaca ggccgtcacg cccgactcgc ggagcaagac gcatttcgtc atcatcgact
      781 gcatcggcgt caccgagacc ccgctggcgg atacgcaccc gctcgaacgc agtaaatcgg
      841 tctcgttcaa aagcctgctg gaacacgtgg cgatgggcgg caccgacccc gagatggtct
      901 catctctggc cgcgcggctg gcccggctcg acaagcagtg cgggccgaaa gagcgtgaga
      961 agatcgccga ggccgcgccg ggagtctcgt tgccgaagat ctgcgccgac atcgtgcatg
     1021 cgctcgaccc cgaccgacag gaagagaaag cccgcgaaac cttcgcgatc cccgccgacc
     1081 aggaacccac cgacgcgcag atcgaagctg cccggaaagc gctggtcaag gaagcggtca
     1141 agccactggc caccaacccg aagctcagaa cgtgtctcga agcactcaag accgaactcg
     1201 aacagatcat cgacacggta acgcccgatg agatcaccca ggcagggttt gcgaaagaag
     1261 cgaaagagaa ggcaaagtca ctcgtacaat cgttcgaaga gtttctcaag gagcatcagc
     1321 gcgagatcga cgcgttgcag ttcttctact cacagccgta tcagcggcgg ttgcgctggg
     1381 cggatatcaa gaagttgcac gaagccattg ccgcgccgcc gcgacagtgg accgaagata
     1441 agctctgggc cgcctacgaa ctgatcgagc agggcaaggt gcgcggcgca cgcgccgagc
     1501 ggagactgac cgacctggtc tcactgattc ggtttgccct gcaccaggag caggagctgg
     1561 tcccgttcgc cgagcggatt cgcgaacgct tcgccgcgtg gctaaaaaac caggaatcgc
     1621 agggccgaac cttcaccgcc gaccagcacc gctggctcga aatgatccgc gaccacgtcg
     1681 cccaaagcta tgagatcagc atcgaagact tcggatacac cccatttgac cgagaaggtg
     1741 gcctcggcaa agccgcccag atcttcggca tgaccaccct gaaaccatta attgatgaac
     1801 ttcacgaggc cctcgtcgca tgaaaagtaa agagatagcc agctggctcg aggtcaaact
     1861 tgaggatgtg atcgaaatcc ttgatcatag acgggaaccc gtcaattcaa cagagcgtga
     1921 atcacgcatc ggaaacgtac catactatgg cgcaacgggc caagttggct ggatagacga
     1981 ctatttgttc aacgaagact tgattcttct tggcgaagat ggggctccct ttcttgattc
     2041 gaagaaaagc gttgcctacc agatccacgg aaaatcttgg atcaataacc atgcgcatgt
     2101 cttgcgtgct atcgctggtt gtccaaatgc ttttttcgct caccaactca atactgttga
     2161 ctatcgccct tttgtc
//
LOCUS       DAORHD010000896         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62739_length_2176_cov_3.762376, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000896 DAORHD010000000
VERSION     DAORHD010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62739_length_2176_cov_3.762376"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKO06_16765"
     CDS             <1..293
                     /locus_tag="PKO06_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016867483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNV71357.1"
                     /translation="LAGDQLYVDFDLSKDNLPPGTRLQIGTAIIEATPPAHTGCSKFV
                     ARFGHDAQQFIHSPLGRQLQLRGINARVLQSGLVRVGDQIRKLAVETNTAQT"
     gene            complement(329..>2176)
                     /locus_tag="PKO06_16770"
     CDS             complement(329..>2176)
                     /locus_tag="PKO06_16770"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV71358.1"
                     /translation="PPKVIRKEMDINRAHLMLGYRTVPLAKTEDSIGLDVLGVILGFG
                     RSSRLSSELKEKKGLVTSIAAGQTSLTDDGLFLVRAEFDPIDEDAVLDGVKEELRRIV
                     REPVGEEELQKAKDFLENAYLRGVESTTGKAESLGGTLMRTDLEYEFKYLERIRAVTA
                     SQLQMLANKYLAGENYVLVLIGPRPRITSAATTEHPVSGKTTKYKLANGMTIVHREIP
                     GSGLVGLTMGVDAGARREPAGKSGISNLTAEMMLKGTKHRDGAKILWDLESLGAELSA
                     GPEPDLVRINLSSSGRNFAKALEIMADVVRNPVFPESAFTVEKNKLLMRLRAIGDDMF
                     ENTWRLFLANMYKGHPYGRYHLGEMDDVEKLTVGDLKQYHARAYVPNNMVIAVVGDVN
                     ASEAIKLIYTSFGALPPGPVWEEDKTLAEVSGVKEFKDVRDVREKKQAFVCLGWLGPK
                     IGHPDYIKVKILNAILGGGMSARYFMNIRNKQSLAYAVTSLFPSRMFGGALLAAIGTD
                     PGMIDKVRDAVLHESEDIIKNGVSAEELARAIAYETGKFALDHDSCSKNASYLAWFES
                     IGAGYQYDDTYLQEAKTVTAEDVKQMAVKYLGQNRALMAVTGPAVGGKK"
BASE COUNT          440 a          669 c          566 g          501 t
ORIGIN      
        1 cgctcgcagg cgatcagctg tatgtcgatt tcgatttgag caaggacaat ctgccacccg
       61 gtacccgcct gcagataggc acggcgatca tcgaagccac tcccccggcc cacactgggt
      121 gttccaaatt cgtcgcccgg ttcggtcacg acgcgcagca gtttatccat tcgccactcg
      181 gcaggcagct ccagttgcgc ggcatcaacg cacgcgttct tcagtcggga cttgtgcgcg
      241 tcggtgatca gatacgtaaa ctcgcggtcg aaacaaacac cgcccaaacc tgaggtctgg
      301 gcggtgttga ttcggacaaa cgaggattct actttttacc accgaccgcc ggaccggtaa
      361 ccgccatcaa ggctcgattc tggccgagat acttgacggc catctgcttc acgtcttcgg
      421 cagtgaccgt tttcgcctcc tgcagatagg tgtcatcata ctgataacca gcgccgatcg
      481 actcgaacca cgccagatac gacgcgttct tcgaacagga atcgtgatcg agagcgaact
      541 ttcctgtttc atacgcgatg gcacgcgcga gttcctctgc cgagactcca ttcttgatga
      601 tgtcttccga ttcatgcagt acggcgtcgc gcaccttgtc gatcatgcct ggatcggtac
      661 cgatggccgc cagcaaggca ccaccgaaca tacgcgacgg aaacagactg gtcaccgcat
      721 aggccagtga ctgcttgttg cggatattca tgaagtagcg agcggacatc ccaccgccga
      781 gaatggcatt cagaatcttg accttgatat agtccgggtg accaatcttc ggaccgagcc
      841 atccgagaca cacgaaggcc tgcttctttt cgcgcacgtc gcgcacgtcc ttgaattcct
      901 tgacgccgct cacttcagcg agcgttttgt cttcctccca gacggggccg ggcggcagcg
      961 caccaaacga ggtgtagatc aacttgatcg cttcagatgc gttcacatcg ccgaccacgg
     1021 caatgaccat gttgttcggc acgtaagccc tcgcatgata ctgtttgagg tcaccgaccg
     1081 tgagcttttc gacgtcgtcc atctcgccca gatggtatcg tccgtatgga tgccctttat
     1141 acatgttggc cagaaagagg cgccaggtgt tctcgaacat gtcgtcgccg attgcgcgca
     1201 gccgcatcag cagcttgttc ttttcgaccg taaacgccga ctcaggaaac accggattgc
     1261 gcacgacatc cgccatgatt tcgagtgctt tggcgaaatt gcgcccggag gagctgaggt
     1321 tgatacgcac cagatcgggt tcagggccgg ctgacagttc cgcgccgagg ctttccagat
     1381 cccagaggat cttcgctccg tcacggtgtt tcgtaccttt gagcatcatc tctgccgtca
     1441 ggttgctgat tcccgacttg cctgccggct cacggcgagc tccggcatcg acacccatcg
     1501 tcaatcccac caacccactg ccaggaatct cgcgatgaac gattgtcata ccatttgcca
     1561 gcttgtactt cgtcgtcttg cctgaaaccg ggtgttcagt cgtcgcggcg ctggtgattc
     1621 tcggtcgagg tccgatcagc acgagaacat aattttcgcc agccagatat ttgtttgcaa
     1681 gcatctgcag ttgcgatgcc gtcacggcgc ggatacgttc gaggtatttg aactcgtatt
     1741 cgagatccgt gcgcatcaac gttccaccga gtgattccgc tttgccggtc gtactttcca
     1801 cgccacgcag gtaggcgttt tcgagaaaat ccttggcttt ctgcagttct tcctcgccga
     1861 ccggctcgcg cacgatgcgg cgcaactcct ccttcacgcc atcgagcacg gcgtcttcgt
     1921 cgattggatc gaattcggct cgcaccagaa acaggccatc atcggtcaac gaggtctgac
     1981 ccgccgcaat gcttgtcacc aagccttttt tctcttttaa ttcactggac aaacggcttg
     2041 accggccaaa ccccagaatg actcccagca cgtcgagccc gatcgagtct tcggttttcg
     2101 cgagaggcac ggtgcgatag cccaacatca ggtgagcccg gttgatatcc atctctttgc
     2161 ggatcacctt cggcgg
//
LOCUS       DAORHD010000897         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62747_length_2176_cov_2.794437, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000897 DAORHD010000000
VERSION     DAORHD010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62747_length_2176_cov_2.794437"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..>2176
                     /locus_tag="PKO06_16775"
     CDS             154..>2176
                     /locus_tag="PKO06_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV71359.1"
                     /translation="MLIRHLSALAAVLVLLLIGLMVKDQMNTPTKTLTIFYTSNLRGQ
                     LKPFVGDSGSYQNLKVGGFSYIGGLIDDLLRKYRSTREFTLLLDTGDSLFGSAEASLT
                     MGEAPYTLMEKLGYDAMCLGNMEFEFGIDALRRFAERNRVPMLACNFRDVKAPLGNTF
                     LPGKIIERAGSRIGVIGLGLTDLPRNTRQENILQIEITDARSAVEKTAAQLRIQGAEL
                     MVLVAHHPGFDDVDKVAAMFPDVDVVIGDQIPLDATQPGRRPLLCATAFPRGAGLGVV
                     RVPYINGVWEPRRGQSFVTIVDATAVSPHAELAAEVNRMEARVDTLLEEVIAEASTDF
                     RRAFNAESTMGNLLTDIVREHASASVALLNSGAIKAAFAQGAVSLGNLFDILPFENSV
                     VRVTLTGAQLEALIEEGLSGRGSFLQASGIDCTYSSLNPPGFRLIQTMIGEEPLEWDR
                     NYQVAVTDFMVDKQSSWPELGHAEKPQAIGLLREAIKEGIRARGTIHVTTEKRFHDVG
                     DQDETLRQQALSIELASLTRPVTASATLHCEYARLAADVLRTETDSDFAFVPVHLIQA
                     SEALQVVSAARLTQDIREVVPVKVAAVTGAQVREILEYAVRNATDPIAFSGLSIETRD
                     GKISLIHPWQGLFDVTKDYKIVFTADLLTKRSEFSTLEALKLQNFSNDLR"
BASE COUNT          453 a          586 c          646 g          491 t
ORIGIN      
        1 tcttgcgaac ggaaaatggg cgagaattaa atgaccctgc tgagacccgg tgagccttga
       61 caggctctct ctcctctggt actgttcgtc agatctcatt ccggccttct aagttccaaa
      121 actcgtctaa agccggcaaa cgaaagtgag cctatgctga ttcgccattt aagtgccctg
      181 gcagccgttc tggttctgct tctcatcggc ttgatggtga aagaccagat gaacacgccg
      241 accaagacgt tgacaatctt ctatacgtca aacctgcgtg ggcagctcaa accgtttgtg
      301 ggtgactcgg gcagctacca gaatctgaag gtcggtgggt tctcatacat cggtggtctg
      361 attgatgacc tgctgcgcaa gtatcgctcg acccgcgaat tcacgttact cctcgacacc
      421 ggcgactcac tgttcggctc tgccgaagcg tcgttgacca tgggcgaagc cccatacacc
      481 ctcatggaaa aactcggtta tgacgccatg tgtctgggaa acatggaatt tgagttcggg
      541 atcgatgcac tgcgccggtt cgccgagcgt aaccgggtgc cgatgctggc ctgtaacttt
      601 cgtgacgtga aggccccgct gggaaacacg tttctcccgg gcaagatcat cgaacgggcc
      661 ggaagtcgca tcggagtcat cggactcggt cttacggatc tgcccaggaa cacgcgtcag
      721 gaaaacatcc ttcagatcga aatcacggat gcacgatcgg cagtcgagaa aaccgcggcg
      781 caacttcgca ttcagggagc agagctgatg gtgctcgtgg ctcatcatcc gggattcgat
      841 gatgtcgaca aagttgcggc gatgtttcct gatgtcgatg tggtcattgg tgatcagatt
      901 cccctggatg ccacgcagcc aggcaggcgg ccgctgctgt gcgcgacggc gtttccccgg
      961 ggagctggtc ttggggtcgt gcgggtcccc tacatcaacg gtgtctggga accccgtcgt
     1021 ggtcagagtt ttgtcacaat tgttgatgcg actgcggttt ctccgcatgc tgaattggct
     1081 gctgaagtga accgtatgga agcgcgggtc gacacgttgc tggaagaagt catcgcggag
     1141 gcgtcgacag actttcgccg cgccttcaac gccgaatcga ccatgggaaa tcttctgacg
     1201 gatatcgttc gcgagcatgc gagcgccagt gttgctctgc tcaactcggg ggcgatcaag
     1261 gcagcctttg cgcagggagc ggtttcgctg ggcaatctgt tcgatattct gcccttcgag
     1321 aacagcgtcg tgcgggtcac gctgacgggg gcgcaactcg aagcgctgat cgaggaaggg
     1381 ctcagtgggc ggggcagttt tctgcaggct tcgggtatcg attgtaccta ttcgagcctc
     1441 aatccacccg ggtttcgcct gattcagacc atgatcggcg aggaacctct cgaatgggac
     1501 cgcaactatc aggttgccgt caccgacttc atggtcgata agcagagcag ctggcctgag
     1561 ctggggcacg ccgaaaaacc gcaggcgatc ggtttgttgc gcgaggcgat caaggaaggc
     1621 attcgcgctc gcggcaccat tcatgtgacg acggagaaac gatttcacga tgtcggagac
     1681 caggatgaga cgttgcgcca acaggctttg agtattgaat tggccagctt gactcgcccg
     1741 gtgacggctt cggcaacctt gcactgcgaa tatgcgcgtc ttgccgccga tgtgttgcgc
     1801 acagagaccg attccgattt tgctttcgta cccgtgcacc tgatacaggc ttctgaagcc
     1861 ctgcaggtgg tttccgcggc tcgcttgacg caagacatcc gtgaggtggt tcccgtgaag
     1921 gtggccgccg tcacgggagc tcaggtgcgc gaaattctcg aatacgctgt ccgaaatgcg
     1981 actgatccga tcgctttttc cggcctgtca atcgaaacca gagacggcaa gatttcgctg
     2041 attcacccgt ggcaagggtt gttcgatgtc accaaagatt acaagatcgt attcacggcc
     2101 gatctgctga cgaagcgcag cgagttctcg actctggaag cgttgaaatt gcagaatttc
     2161 agcaatgatc tgcggc
//
LOCUS       DAORHD010000898         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62855_length_2173_cov_3.734655, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000898 DAORHD010000000
VERSION     DAORHD010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62855_length_2173_cov_3.734655"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKO06_16780"
     CDS             complement(<1..665)
                     /locus_tag="PKO06_16780"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNV71360.1"
                     /translation="MNRVLSIVSLVVFTVLLAVTPACAQEDGDKPVTIPQPLKEWTFA
                     VFLNADNNLDPFGVEDQVEMSKIGSSNWLNIVTLIDRERGPAQWNYIEKGKVTKVKDM
                     GELDMGDYKQFIDFVKFVKTNFPAKKYMIGIWNHGSGWKAAGKIVRGVSYDDSTGNHI
                     TTNQLVGALGEAKKILGKNIDILNFDCCLMQMAEVVYACKDSCDYIVASEETEPGKGT
                     PYD"
     gene            1149..>2173
                     /locus_tag="PKO06_16785"
     CDS             1149..>2173
                     /locus_tag="PKO06_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71361.1"
                     /translation="MRYRSGRFSLLLWCLMAIVPAVSVWADISNLACTVTTPTDFSKN
                     YNNNQEISLSATWQGDTSPYAATFKANGASIGTSNTDGTTTTFSLSAASLREGDNTFS
                     VSVIETSVPGATSSGDENANGTVTIDRTPPTLTLTVTSGNIVSPNTGTNEVMLQFTSS
                     EGLGEQPQFTISPGAIGAPTPVGTPSPPFNSGQYRITVPNGTSGGTYVVTVTGRDNTE
                     PASGRNVGTAQTSFVVDAAADGVPTINSANPPSPCRSQNVVLSGSLPVETGAQRVEVL
                     EGSAVKATTSVAANTDSWTVTLTDVPEGSHKYSARRIDPLGNVSGTGAELTVIVDRTA
                     PTVPSLAS"
BASE COUNT          486 a          616 c          598 g          473 t
ORIGIN      
        1 tcatcatacg gggtgccctt gccgggttcc gtttcttctg aggcgacgat gtaatcgcag
       61 ctgtctttgc aggcatagac cacttcggcc atctgcatga ggcagcagtc gaagttcaga
      121 atgtcgatgt tcttgccgag aatcttcttg gcttcgccga gagcaccaac caactgattg
      181 gtcgtgatgt ggttgccggt cgaatcgtcg taagaaacgc cgcgcacgat cttgccggcg
      241 gccttccagc ctgagccgtg gttccagatg ccgatcatgt acttcttggc gggaaagttc
      301 gtcttcacga acttcacgaa atcgatgaac tgcttgtaat cgcccatgtc gagttcgccc
      361 atatccttga ccttggtcac tttgcccttc tcaatgtaat tccactgggc gggaccacgt
      421 tcacggtcaa tcagggtgac aatattcagc cagttgctgg agccgatctt cgacatttcg
      481 acctggtctt caacgccaaa cgggtcgagg ttgttgtcgg cattcaggaa aaccgcgaag
      541 gtccattcct tgagcggttg ggggatggtg acgggtttgt ctccgtcttc ctgggcgcaa
      601 gccggggtca cggcaagcaa tacggtgaaa actacgaggg aaacaatact caatactcta
      661 ttcatctcgc cctccttatg agcttattcc cagcttatgg gaactgatga aaaacgcctt
      721 ccccatcggg aagatacaaa ttgattgtaa cagccaccgg ccccaacccg caagggtacg
      781 gcgcaacttt ttttgctcaa gacccctact caaggaaagt ataatcagat aggcacattc
      841 tgaatgatgg ggcacagata catgaaggga atcgcgaatc gtcagaacac cttgttagga
      901 agaaagccga tgccgtttgg cggtggtcgg ccgactccga aaaacaaact tggtgttcgg
      961 gctcgggcgt ggcatccttg tcctcgagat cgcgagagaa cttctgaagc gaaagcccct
     1021 gagttccgaa caatgcacca agaagaccca gtggctctcg tttccccgca acctgagtca
     1081 ggtaaaactg ctgatagtgc tcctcttgac cagattcacg agaaacccga agctgaggag
     1141 gccgaaccat gaggtatcga tccggccgtt tttccttgct gctctggtgc ctgatggcaa
     1201 ttgtcccggc cgtttcggtg tgggccgaca tcagcaatct tgcctgtacg gtcacgacac
     1261 cgacggattt ttccaaaaac tacaacaaca atcaggaaat cagcctgtct gcgacctggc
     1321 agggagacac ctcgccgtat gctgcgactt ttaaagcgaa tggcgcttcc atcggcacgt
     1381 cgaacaccga tggaacgacc acgacctttt ctttgtcggc agccagttta cgcgagggcg
     1441 acaacacgtt ttcggtttcg gtcatcgaaa ccagtgttcc gggggcgacg agttcgggcg
     1501 atgaaaacgc caacggtacc gtgacgattg atcgaacccc accgacctta acgctgacgg
     1561 taacgtcggg caacatcgtc agcccgaata cgggcaccaa tgaagtgatg ctgcagttca
     1621 cctcttcaga agggttgggc gagcagccgc agttcacgat cagccctggc gcaatcggtg
     1681 cgccgacacc ggtcgggact ccgagcccgc cgtttaacag cgggcagtat cgcatcacgg
     1741 tacccaacgg gacctcgggt ggtacgtatg tggtcaccgt cactggccgc gacaacaccg
     1801 aaccggcgag cggtcgaaat gttggcaccg cccagacttc ttttgtggtc gatgccgcag
     1861 ccgatggagt tccgacgatc aactcggcga atccaccgtc accgtgtcgc tcgcagaatg
     1921 tcgtactttc cggttcgctg ccggtggaaa ccggagctca gcgtgttgag gtgctcgaag
     1981 gctcagccgt aaaagcgacc acgagcgtgg cggcgaacac ggattcctgg accgtcactc
     2041 tgaccgacgt tccagagggc tcacacaagt actccgcccg acgtatcgac ccgcttggga
     2101 atgtttccgg cacgggagcc gaactgacgg tgattgtcga tcgaacggca ccgaccgtac
     2161 cgtctctggc gtc
//
LOCUS       DAORHD010000899         2170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_62979_length_2170_cov_4.760284, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000899 DAORHD010000000
VERSION     DAORHD010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62979_length_2170_cov_4.760284"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKO06_16790"
     CDS             <1..533
                     /locus_tag="PKO06_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV71362.1"
                     /translation="FDEGIRGALQTDVDVIMVGELRDPEAVNMTLTAAECGKLVFATL
                     HSNTAMQAIDRFVNLFPQTQQEQILSRLSNCIIGIITQTLLPRKNTKGRVAAFELLLG
                     LPMIRGLIAERRVHLISSYLESGGETGMCTLDQSLAWLVATGKVLPEEAIMRASSQSG
                     LKKLIEQAAEGGGKPV"
     gene            530..1573
                     /locus_tag="PKO06_16795"
     CDS             530..1573
                     /locus_tag="PKO06_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV71363.1"
                     /translation="MMPKIFRYFDQAIEMGASDLNLKPGAPPVFRVNGHLIISEDDTL
                     VPQEMYDLFLPLIDAAKQQNLRNIFEVYAMYRYKRSARIRYYLFQQREGYAGSFRFIN
                     PHVPTIEEIGLPAILDSEAAKPRGLFLVTGPAGSGKSHTMAAMVQSINRKFARHIITM
                     ENPIEFVFKPQQSIFTQIEVGTQIPTYQAALTNALREDPDVMMIGEMRDQKTVEMALV
                     ASETGHYVLSTLPTLGAASTIERIVSFFPPDKHEEVRLQISLNLIGIFSQLLLPKVTH
                     EARASVAYELLIANPAIRTLIRDKKYSQLQSAMLMARREGCVTLKDSLTRLMREESVN
                     QPLIKAMLQEIVE"
     gene            1570..>2170
                     /locus_tag="PKO06_16800"
     CDS             1570..>2170
                     /locus_tag="PKO06_16800"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71364.1"
                     /translation="MNDQVQSKINSLYDKVAARLFPILKADLPPKIKLKTLRAISRTK
                     IPASRQVVFEQFAQFSDEEDAIRAVGELGGEDAAQALIARVRQPHGDNRDVMAEQLGR
                     FKYPFVRECLTELLGDPDRHVRYQAVFSLFSFGGRDAALALCRYISDPDEWISMNILK
                     ILCRMKEHESIPFLADQFAKDQDLRRKAQILDRGASRLSE"
BASE COUNT          475 a          672 c          588 g          435 t
ORIGIN      
        1 ccttcgacga gggcattcgc ggcgctctgc agaccgatgt tgatgtcatc atggtcggcg
       61 agttgcgcga ccccgaagcc gtcaacatga ccctgaccgc cgccgagtgc ggcaaactgg
      121 tcttcgccac cctgcacagc aatacggcca tgcaggccat cgaccgcttc gtgaacctgt
      181 ttccccagac gcaacaggaa cagattctca gccgtctcag caactgcatc atcggcatca
      241 tcacgcagac gctgctcccc cgtaagaaca ccaaggggcg cgtcgctgct ttcgaactgc
      301 tgctcggatt accgatgatc cgcggcctga tcgccgaacg ccgcgtccat ctgatttcgt
      361 cgtatctcga atcgggcggt gaaaccggga tgtgcacgct cgaccagtca ctggcctggc
      421 tcgtcgcgac aggcaaggtg ttgccggaag aggccatcat gcgtgcctcg agccagtcag
      481 ggctcaagaa actcattgaa caggctgcag aaggcggagg aaaacccgta tgatgcccaa
      541 aattttccgc tacttcgatc aggcgatcga aatgggtgcg agcgacctca acctcaaacc
      601 cggtgcaccg ccggtctttc gggtcaacgg gcacctcatc atttccgagg atgacacgct
      661 cgtacctcag gaaatgtatg acctgttcct gccgctcatc gatgctgcca agcagcagaa
      721 cctgcgcaac atctttgaag tctacgccat gtaccggtac aaacgcagcg cgcgcattcg
      781 ctactacctg ttccagcagc gggaaggcta cgccggttcg ttccgtttca ttaacccgca
      841 tgtgcccacg attgaggaaa tcggtctgcc ggcgattctc gattctgaag ctgccaagcc
      901 gcgcggtctg tttctggtca ccggccctgc tggcagcggg aaatcgcaca ccatggccgc
      961 catggtgcaa tcgatcaacc gcaagtttgc gcgccacatc atcacgatgg aaaaccccat
     1021 cgagtttgtc ttcaaaccgc agcagtccat tttcactcag atcgaagtcg ggacgcagat
     1081 tccgacctat caggcagctt tgaccaacgc gctgcgcgaa gaccctgacg tcatgatgat
     1141 cggcgaaatg cgcgatcaga aaaccgtcga aatggctctg gtcgccagtg aaaccggtca
     1201 ctacgttctc tcgacgctgc cgacgcttgg cgccgcgtcc accatcgaac gcatcgtcag
     1261 cttcttcccg cccgacaagc acgaggaagt tcgactgcag atctcgttga acctgatcgg
     1321 catcttttct caactgctgc tgcccaaggt cacacacgag gcgcgtgcca gcgttgcgta
     1381 cgagttgctg attgcgaatc cggctatcag aacgctgatt cgagacaaga aatacagtca
     1441 gttgcagagt gccatgctca tggcacggcg tgaaggctgc gtgacgctga aagactcgct
     1501 gacccgactg atgcgcgagg aaagcgtgaa tcagccgctc atcaaggcca tgttgcagga
     1561 gatcgtcgaa tgaacgacca ggtgcaatcg aaaatcaatt cgctgtatga caaagttgcc
     1621 gctcgtcttt ttcccattct caaggccgat ctcccaccga agatcaaact caagacgctg
     1681 cgggccattt cgcgaaccaa gattccggca agccggcagg ttgtcttcga gcagtttgcc
     1741 cagttttcgg atgaagaaga tgccattcgc gccgtcggcg agctcggtgg agaagatgcc
     1801 gcccaggctc tgatcgcccg ggtgcgacag ccgcacggcg acaaccgcga cgtcatggcc
     1861 gaacagcttg gtcgcttcaa gtacccattc gtgcgcgagt gcctgaccga actgctcggc
     1921 gatcccgatc gacatgttcg ctatcaggcc gtgttcagcc tcttcagttt cggcggccga
     1981 gatgcggctc tggccctctg ccgctacatt tccgacccgg acgaatggat ctcgatgaac
     2041 attctgaaga ttctgtgccg catgaaggag catgaatcca tcccgttcct cgccgatcag
     2101 ttcgccaaag accaggatct tcgccgcaag gctcagatcc tcgaccgagg tgccagcagg
     2161 ctgagcgagg
//
LOCUS       DAORHD010000900         2169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63030_length_2169_cov_5.125828, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000900 DAORHD010000000
VERSION     DAORHD010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63030_length_2169_cov_5.125828"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKO06_16805"
     CDS             complement(<1..337)
                     /locus_tag="PKO06_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71365.1"
                     /translation="MKSSSPLDTYKHIFAELFAKQVTFLLDDEFAEALQDSVIFFHNL
                     EEGETFDFDPEQEFLFLAWFLLDDSDAENHTLLEVFLERFSDELSMQEKQICHAMRDT
                     HLSLLELRSI"
     gene            complement(620..1615)
                     /gene="pseG"
                     /locus_tag="PKO06_16810"
     CDS             complement(620..1615)
                     /gene="pseG"
                     /locus_tag="PKO06_16810"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HNV71366.1"
                     /translation="MSKVLIRADGGATVGMGHLMRCRTLAIALQLRGWKTVFLSEGVP
                     GEGFADDQGRKFEVVDPSRSGTNGAGTAQVAEQTQADLVLIDTYTFGMKDFLALWKSG
                     RPLVVIDDLADRGLPVDAVINPNPLFPDTRYREQGIPHCLIGSDYCLIRPEVLVARQL
                     SVRQERHLLVTLGGGGVPEVLLPLLPIMESSDFHQITVMASTSAPKLEAWVQAHATRW
                     QICRDFSSLPRLVAEADAVVSAGGTTLWEVYCVGRPSLAIVWVDNQRHALRVVDAQRT
                     GTVVDLRRGFDEQVFTRAWQEFLDSRQDQDCIERQQQLIDGRGAERAAEVLLRLG"
     gene            complement(1615..>2169)
                     /locus_tag="PKO06_16815"
     CDS             complement(1615..>2169)
                     /locus_tag="PKO06_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HNV71367.1"
                     /translation="EDVLARFLDTVKLWPTRHVVRLTADCPFHDPGVLDALVDLHLRG
                     DYDYSSNLHPPTYPHGLDAEVVRREVLEMADRESTAPSHREHVTLFLREHADRFRCGN
                     LTFGNDVSAYRLTLDRPEDYRFLQAFMTRVADLDSIPSIYEIVRLLELFPELVTINGS
                     LERYEWKKTIREREARDVNIGRTS"
BASE COUNT          544 a          640 c          561 g          424 t
ORIGIN      
        1 tgatcgatcg caactcgagc agggaaagat gcgtatcgcg catggcatgg cagatctgct
       61 tctcctgcat cgaaagctcg tctgaaaagc gctcgagaaa gacttcgaga agcgtgtgat
      121 tctcggcatc actgtcgtcg agcagaaacc aggccaggaa cagaaattcc tgctccgggt
      181 caaaatcaaa cgtttccccc tcttccagat tatgaaaaaa gatgaccgaa tcctgcaacg
      241 cttcggcaaa ctcgtcatcg agcaaaaacg tgacctgctt ggcgaacagt tcggcaaaaa
      301 tgtgcttgta ggtgtccaga ggggaagacg acttcatgag acggtccttt gactgatact
      361 aggcccgggt gggctcgatt tgaggctcac ggacatggag acgacgctcg cgacggataa
      421 ccctgctgca tccctctctt cctgttgtga gactgaacca ccacgagaga ggaacaatgc
      481 gaatttccga atccatgcgg ggcacgagag tcagacgatc catggctgcc acctttcgat
      541 aaggataaga gcagtccctg tatcggcatg gttctcgaaa ttccccacag acaggatacc
      601 tcggttcgac cgtcagtcgt caacccaaac gcagcagaac ctcggccgct ctctcggcac
      661 cacgaccatc aatgagctgc tgctgtcgct caatgcagtc ttgatcctgg cgcgagtcaa
      721 gaaactcctg ccaggcccgg gtgaaaacct gctcgtcaaa cccgcggcgc aggtcgacaa
      781 ccgtccccgt ccgctgcgcg tcgacgaccc gcaacgcgtg tcgctgattg tccacccaga
      841 ctatggccag actcgggcgg cccacgcagt agacttccca cagagtcgtg ccaccagcgg
      901 aaaccaccgc gtccgcttct gcgaccaatc gaggcagcga cgagaaatcg cgacagatct
      961 gccatcgcgt cgcgtgagcc tgtacccagg cttcgagttt tggagccgat gtcgaagcca
     1021 tgaccgttat ctgatggaag tcactcgatt ccatgatcgg caacagaggc aacaagactt
     1081 cgggcacgcc accgccaccc agggtcacga gcagatgccg ctcctgcctg accgacagct
     1141 gacgagcgac gagcacttcc ggtcgaatca aacaatagtc tgatccaatc agacaatgtg
     1201 ggataccctg ctcgcgataa cgggtatcag gaaaaagtgg attcggattg atcaccgcat
     1261 cgaccggcag gccccgatca gcaagatcgt cgatcaccac gagcggtcga ccgctcttcc
     1321 acaacgcgag aaagtccttc atgccgaacg tgtaggtatc gatgagtaca aggtcagctt
     1381 gtgtctgttc ggcaacttga gctgttccag cgccattcgt acctgatcga gacggatcaa
     1441 cgacctcgaa cttccggccc tggtcatcgg caaaaccttc accgggaacg ccttcagaaa
     1501 gaaagaccgt cttccagcct cgcagctgaa gagcaatcgc cagagtgcga cagcgcatca
     1561 gatgacccat gccgacggtc gcgccgccgt cggcacgtat gagcaccttc gacatcagga
     1621 ggttctgccg atgttcacat cgcgcgcttc tctctcacga atggttttct tccattcgta
     1681 tcgttcaagt gagccgttta tcgtgaccag ctcagggaac aattccagta atcgtacaat
     1741 ttcataaata ctcggaatcg agtcaagatc ggccacacgg gtcatgaatg cctgcaaaaa
     1801 acgatagtct tcgggccgat cgagcgtcag acgataggca gagacatcat taccaaacgt
     1861 caggttccca cagcgaaatc gatcggcatg ctcacgcaga aacaaagtga catgctcccg
     1921 atgcgacgga gccgtacttt cccgatcagc catttcgagc acttcacgcc tcaccacttc
     1981 agcatcgagg ccatgcggat acgtgggcgg atgcagattc gatgaatagt catagtcacc
     2041 gcgaagatgc agatcgacaa gcgcatccag aactccagga tcatggaatg gacaatcggc
     2101 ggtcagtctc accacgtgcc gtgttggcca gagttttacg gtatcaagga atctcgccag
     2161 aacatcttc
//
LOCUS       DAORHD010000901         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63140_length_2167_cov_3.919034, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000901 DAORHD010000000
VERSION     DAORHD010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63140_length_2167_cov_3.919034"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PKO06_16820"
     CDS             <1..668
                     /locus_tag="PKO06_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNV71368.1"
                     /translation="NVLFTATVSPMPFLLRFGALLVLMLASVAANGSALLFFWVPLTV
                     LYRRLACETWNGLGRELVLSAWFALLIGTLALPTGGKPAVVLIEDAMLRLFIFVSSSQ
                     TFSRSLYPREGVWWLSRFLPGAATLTLEMALRFLPLLAHEIQDIVRIQRARGAFTDRE
                     WLVRLRALILPFFVRIFRMSERVEFALSARGIDPERPRCVVEPRLVRELVQTIEKEHA
                     CPR"
     gene            656..1465
                     /locus_tag="PKO06_16825"
     CDS             656..1465
                     /locus_tag="PKO06_16825"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HNV71369.1"
                     /translation="MSTLIQRIKERIPADRLLIKPDLVAIYSFFTAVRSYRLGLSMTL
                     LDEDIFPEGVHYPVRHMGTLEQVPLGELLTWTESPNGLERSIGMAALNSCLPLAELSF
                     HEGNALELAAHWGEGKNVVVIGHFPHLERIRERASSMVILEKRPQPGDLPAEEAPNVI
                     PHADVVALTGVTCLNDTIEGILALKKPGARFIALGPTIPLSSLLFDAGIDVVGGAWVD
                     DETRALPMLAQGATARLLKGTRNVLMAREPNLLPATFTSILPPTEREKEPA"
     gene            1468..2154
                     /locus_tag="PKO06_16830"
     CDS             1468..2154
                     /locus_tag="PKO06_16830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV71370.1"
                     /translation="MDQPVRSLCLESDIAIPSWDGEKAGSFQEFADEVFAPIYPYICR
                     DIETAWGRSLEARRVLEIGGGPGNMALELLNRGVASLTGLDISEAMLERTSRRLRAAG
                     LEGKFRAVPGEASTLPFAERSFDLVFSRGSIQFWPDIPRALREIARVLSIDGFAFVGG
                     GFGLSTPTEVKQSIHAERNRRMAERPDLHPIPKLNHDEVLACALGIGGKAELAHDGPG
                     HWLLWYPAAK"
BASE COUNT          377 a          645 c          665 g          480 t
ORIGIN      
        1 tgaacgtgct atttaccgct actgtttcgc caatgccctt tctgctgcgg ttcggtgccc
       61 tgctggttct catgctggcg tccgtggccg cgaacgggag cgctctgctg ttcttctggg
      121 tgccgctgac cgtgctgtat cgacgcctcg cctgcgaaac ctggaacgga cttggtcggg
      181 aattggttct gtcggcctgg tttgctttgt tgatcggaac gctggctctg ccgaccggag
      241 gcaaaccggc cgtggtgctg atcgaagatg cgatgctgag actgttcatc tttgtctcgt
      301 cttcgcagac cttctcccgc tccctgtacc caagggaagg ggtatggtgg ctgtctcgtt
      361 tcctgcccgg cgcggcgacc ctgacgctcg agatggccct gcgatttctg ccgttgctgg
      421 ctcatgagat acaggatatc gtccgcattc aacgcgctcg aggagccttt acagatcgcg
      481 aatggcttgt acgcctgcgc gccctgatac tgccgttttt tgtccggatc tttcgcatgt
      541 ccgagagggt cgagtttgcc ctttccgccc gaggaattga cccggagcgg cctcgttgcg
      601 tcgtcgagcc gcgactcgta cgagaactcg tacaaaccat cgagaaggag cacgcatgtc
      661 cacgttgatt cagcgcatca aggaacgtat tccggccgac cgattgctca tcaagccgga
      721 tctcgtcgcc atctattcgt ttttcaccgc ggtacgcagc tatcgcctgg gtctcagcat
      781 gaccctgctc gacgaggata tcttcccgga aggtgtccat taccccgtac gccacatggg
      841 gactctcgaa caggtgccgc tcggcgaact gctcacctgg accgaatctc cgaatggtct
      901 cgaacgctcg atcggcatgg ccgcgctcaa ctcgtgtctg ccgctggctg agctgtcgtt
      961 tcacgaagga aatgcgctgg agttggccgc tcactggggc gaagggaaga acgtggtcgt
     1021 gatcggccat ttcccgcatc tcgagcggat tcgcgaacgg gcttcctcga tggtcatcct
     1081 ggaaaaacga ccgcagccgg gagacctgcc cgccgaagag gctccgaacg tgattccgca
     1141 cgctgacgtg gtcgctctca ccggggtgac ctgcctcaac gacacgatcg aaggcattct
     1201 ggcattgaag aagccgggag cgcgtttcat cgcgctcgga ccgacgattc ctctgagctc
     1261 gttgctgttc gatgcgggca tcgacgtggt cggcggcgcg tgggtcgatg acgagacgcg
     1321 ggctttgccc atgctcgctc agggagccac cgcccgcctg ctgaagggta cccggaacgt
     1381 gctcatggct cgcgaaccga atctgttgcc cgcgactttc acttcgattt tacccccgac
     1441 ggaaagagaa aaggagcctg cataatcatg gatcaacctg tacggtcgct ctgccttgaa
     1501 tcggacattg ccattccctc gtgggatggt gaaaaagcgg ggtcgtttca ggagtttgct
     1561 gacgaggttt tcgcgccgat ctatccgtac atctgccgtg acatcgagac ggcctgggga
     1621 cgatcgctcg aggcaagacg agtgctcgag atcggcggcg gtccgggcaa tatggctctc
     1681 gaactgttga accgcggggt ggcctcactg accgggctcg acatttctga agccatgttg
     1741 gaacgtacca gtcgtcgtct gcgcgccgcc ggtctggaag gaaagttcag ggcggttccg
     1801 ggggaagcgt cgacccttcc cttcgccgag cgatcgttcg atctggtgtt cagtcgcggg
     1861 tcgatccagt tctggcctga tattccccgg gctctgcgtg agatcgcccg cgtgctctcg
     1921 attgacggct ttgccttcgt cggcggcggg ttcggtctga gcacgccgac cgaggtgaag
     1981 cagagcattc acgctgaacg gaatcgccgc atggccgaac ggcctgatct gcacccgatt
     2041 ccgaaactga atcacgatga ggtgctcgcc tgcgccctcg gtatcggggg caaggcggaa
     2101 ctcgcacatg acgggcccgg gcactggctt ctctggtatc ccgcggcgaa atgaaccgat
     2161 agaaaaa
//
LOCUS       DAORHD010000902         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63194_length_2166_cov_3.347703, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000902 DAORHD010000000
VERSION     DAORHD010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63194_length_2166_cov_3.347703"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKO06_16835"
     CDS             complement(<1..649)
                     /locus_tag="PKO06_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71371.1"
                     /translation="MTLGLLIRRSLAQHWMSTLAAVLSLALGIGLMTAVLSLRQETHR
                     QFTRVGLGVDAVLGPKGSPLQIVLNAIYNLEEMPGKIAWPLVEELRKHDVVERVIPFC
                     TGHSFGGFRVNAIERDFFSGFEIQPGKTLSFDATAGGQGRAFAAGNVNEAVIGWEAAR
                     RLGVKLGDSFNPVCGVNQNDPEHHQDQLVFVGVLARTGTPYDRVIYIPLERFYTLG"
     gene            complement(646..1335)
                     /locus_tag="PKO06_16840"
     CDS             complement(646..1335)
                     /locus_tag="PKO06_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71372.1"
                     /translation="MSQVLRLHECRYAVSGPQGIIEILHIPAFTVNAGERVVLQGPSG
                     SGKTTLLHLLSGLLCPSSGEVEVAGVRVDQMTEIERDAFRARHIGYIFQSFNLLPGLS
                     AIENIQLGMSLSRGKVDPERAHAMLAEVQLSHRLHHRPDQLSIGEQQRVAVARALACE
                     PAVLLADEPTGSLDDVRAKEVKTLLESATTKFGCTLIAVSHDPSVVQRFPRQIDFATL
                     NCSYRQAGVAA"
     gene            1513..>2166
                     /locus_tag="PKO06_16845"
     CDS             1513..>2166
                     /locus_tag="PKO06_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71373.1"
                     /translation="MERSENPAEETASECVETGPRFALRLALDEFLHALAGLLPGFLT
                     AERPRSMAETFIVRKTGVSELARVRELQLYLGPTAEPAVVGKRQLQLWIRGTWTREQL
                     LAQLQQPGGPVTVDRQEGQEILVFRHLPDFVGWCAADGALVVASRDLIKGSRFQLDVP
                     ILSPEKEHILRRHPLAFCARRGGPWWQACARWVNSRLARDRDDLAQALDEAARHVCGY
                     "
BASE COUNT          439 a          605 c          689 g          433 t
ORIGIN      
        1 caccgagcgt gtagaaccgt tcgagtggaa tgtagatgac acgatcatag ggagtccccg
       61 tgcgggcaag caccccgacg aataccagct ggtcctggtg gtgttccggg tcattctgat
      121 tgacaccgca gaccgggttg aaggagtctc cgagcttcac gcccagccgt ctggcggctt
      181 cccagccgat cacggcttcg ttgacgtttc cggcagcaaa cgctcgcccc tggccgcctg
      241 ccgtcgcatc gaacgagaga gtcttccccg gttggatctc gaaaccggaa aaaaagtctc
      301 gctcaatagc attcactctg aatcccccaa atgagtgacc agtacaaaag ggaatgacgc
      361 gttcgacgac atcgtgtttt cgcagttcct ccacgagcgg ccaggcgatc ttcccgggca
      421 tctcttccag attgtagatg gcattcagga caatctgcag cggcgatcct ttggggccga
      481 gcacggcatc aactcccaag ccgacccggg tgaactgacg gtgggtttcc tggcgaagag
      541 agagaacggc cgtcatcagg ccgatgccga gcgcgaggct gagaacggcg gccagcgtag
      601 acatccaatg ctgagccaga ctgcgtctga tcagtaagcc gagcgtcatg ccgcgactcc
      661 ggcctggcga tacgagcaat tgagtgtcgc gaagtcgatc tgtctgggaa accgctgaac
      721 cacggacgga tcgtgactga cggcaatcag cgtgcaacca aactttgttg tggcgctttc
      781 cagtaaagtc ttgacttcct tcgcgcgcac atcgtccaga ctgccggtcg gttcatcggc
      841 gagcagcacc gcgggttcgc aggccagggc gcgtgccacg gccacgcgct gctgctcccc
      901 aatggaaagc tggtcgggcc gatgatgcag gcgatggctc agttgtacct ccgccagcat
      961 ggcgtgcgct ctttcggggt cgactttccc tcgggaaagg ctcattccca gttgaatgtt
     1021 ctcgatagcg ctgaggccgg gcagcaggtt gaaggattga aagatgtacc caatgtggcg
     1081 agctcgaaac gcgtctcgtt cgatctcagt catctgatca actcgtactc ctgcgacttc
     1141 gacttcgcca ctggaagggc agagcagtcc ggaaagcaga tgcaggaggg tggtcttgcc
     1201 cgagcctgat ggcccttgaa gcacaacgcg ttcgccggca ttgaccgtga acgccggaat
     1261 gtggagaatc tcgatgattc cctggggtcc cgaaacggca taccggcatt catgcaggcg
     1321 tagaacctga gacaatcgct cctccagaag ttactcgtga ctccaacagg aaacatgtcc
     1381 tgaggcaaac tgtcaaattt cctccggcga gcagcagggg gcaaaaagaa tgctgtggta
     1441 tgactgcgca gcgtgtatca tgtagagtca gggtggcagc cgatacgcga aggatgaatg
     1501 gaggtcaggg acatggaacg cagcgaaaat cccgccgaag agacggcgag tgagtgtgtg
     1561 gaaaccgggc cgcggtttgc tctgcgactg gcgctggatg aatttctgca cgcgctggct
     1621 gggctgctcc cggggttttt gaccgcggaa cgcccgcgtt caatggcgga gacctttatt
     1681 gtcaggaaga ccggagtttc tgagttggcg agggttcgag aactgcaatt gtatctcggg
     1741 cccacggccg agccggcggt ggtcggaaag cgtcagctgc agctgtggat ccgtggcacc
     1801 tggactcgcg agcaactgct ggctcagctg cagcagcccg gtggaccggt caccgtcgac
     1861 cggcaggaag ggcaggagat cctcgtgttc cgccatcttc ctgactttgt cggctggtgt
     1921 gccgcggatg gagccttggt ggttgcctcg cgtgatttga tcaagggctc gcgatttcag
     1981 ctcgacgtac cgattttgtc gcccgagaag gaacatattt tacgtcggca tccgctggcg
     2041 ttctgtgcgc ggcgcggtgg cccctggtgg caggcatgtg ctcggtgggt gaactcacgt
     2101 ctggcgagag atcgagacga tctggcgcaa gcactggacg aggcggcgcg ccacgtctgc
     2161 gggtat
//
LOCUS       DAORHD010000903         2161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63425_length_2161_cov_3.662393, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000903 DAORHD010000000
VERSION     DAORHD010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63425_length_2161_cov_3.662393"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="PKO06_16850"
     CDS             complement(<1..1641)
                     /locus_tag="PKO06_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71374.1"
                     /translation="MRWFRNLRRVLLCVSLLCLPLEAAVRINTILCDRTNVSKGQTGI
                     EIYMEVENLDAAPATLVMASLTFSLGSYEVTLNNPSLGSAIPGLGKTTCVFTLSVDPL
                     SPSGVCVIDGSVQTSGGNDNSSDLTHSWSVQQPSELVITSIVGPSEVNRGSADNQAIM
                     DVARTGEADVNLNHVDLTPLVPTNYTNWIRTSPTLPQNFAKAYWWNPRWQYRRQLTIA
                     NRSTSTLPTRYEIAFSFDHAALITAGKSLPSGDDIRIVYDNGTAFQEIPRYLDPMQSA
                     WASSTTTIWFPLQEPLGPAPAATQRYTLYYGASIMDAALPPANPANVFIFSDDCENGA
                     NGWTTNFLAGYPTTRWELGPTGPQPGGDPDGLTHPPNTTIYWPTGAYSPNNCWATDLD
                     GYYRGRNPAAEVVFLYSPTIDLRDTLNPTLTFYNFLDVEDPTTPFDWGTLRIRQESAP
                     RPGPVLRTLLNRHIDETETGWRQESFSLLPELGKQAYLEFALRVDQLVLGYGWAIDDI
                     RVRQIAVPEPNNPLLGVEEIVPSVPTIKAYFDVDVSPTA"
     gene            complement(1644..>2161)
                     /locus_tag="PKO06_16855"
     CDS             complement(1644..>2161)
                     /locus_tag="PKO06_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71375.1"
                     /translation="AEPPLLALSGTFEGTAPASVVPSRTLTPQEKAVAEACLIEASRL
                     IDRGSDAKALDWLRRAHAAAPGEPRYADALRIHENQIAARSSLRVLEDKLRAGKADEA
                     WEEYLSKALHDRFYFLSTASRWSRILDRHGQTASAGSVLRTYLTCKPTDLSAQQRYQR
                     LLIRLGCLSGV"
BASE COUNT          461 a          559 c          673 g          468 t
ORIGIN      
        1 tgccgttggc gagacgtcga cgtcaaaata ggctttgatg gtcggaacgc tggggacgat
       61 ctcctcaacc ccgaggagcg gattgtttgg ttcgggcacg gcaatctgcc gcacgcgaat
      121 atcatcgatt gcccatccgt acccgagaac cagctggtcg actcgcaggg cgaactcgag
      181 atacgcttgt ttccccagtt caggaagcag ggaaaacgat tcctgtctcc accccgtttc
      241 cgtctcgtcg atgtggcgat tcagaagcgt tcgcaatacc ggtcccggac gtggtgcaga
      301 ttcctgacgg atacgcagag ttccccagtc gaacggagtg gtcggatcct cgacatcgag
      361 aaaattgtaa aacgtgagcg ttggattgag cgtgtcacga agatcgatag ttggactgta
      421 caagaacaca acttcagccg cggggtttcg acctcgataa tacccgtcga ggtcggtcgc
      481 ccagcagttg ttcggactgt aagcgccggt gggccagtag atcgttgtgt tgggtgggtg
      541 ggtcagacca tccgggtctc ccccgggctg cgggcctgtc gggccgagtt cccaacgagt
      601 ggttgggtat ccggcgagaa aattggtggt ccagccgttc gctccgtttt cgcaatcgtc
      661 gctgaaaatg aatacattgg ctggattcgc cggtggcagg gcggcatcca tgatcgaagc
      721 accgtaatac agagtgtatc gttgcgttgc tgccggggcc gggccgagag gttcctgcaa
      781 aggaaaccag atggtcgtgg tcgaactggc ccaggcgctc tgcatcggat caaggtagcg
      841 cggaatttct tgaaacgcgg tgccattatc atacacgatg cggatatcat cgccactggg
      901 gagcgacttg ccggcagtga tcaatgccgc atggtcaaag gaaaaggcaa tttcgtatct
      961 ggtcggcagc gtactggtcg accgattggc aatcgtcagt tggcggcgat actgccagcg
     1021 tggattccac cagtaggcct tggcgaagtt ctgcggtaat gtcggcgaag tccgaatcca
     1081 gttcgtgtaa ttcgttggaa cgagcggcgt gaggtcgaca tgattcagat taacgtcggc
     1141 ctcgccggtt cgcgccacat ccatgatggc ctgattatcc gcgctgccgc gattgacctc
     1201 tgatggcccg acaatcgaag tgatcacgag ttccgaaggt tgctgcacgg accaggagtg
     1261 cgtcaggtca gagctgttgt cattgcctcc cgaggtttgc accgatccgt caataacgca
     1321 cacgcccgaa ggggaaagag gatcgacgga cagggtgaag acacaggtcg tttttccgag
     1381 acctggaatt gcgcttccaa ggctggggtt gttgagcgtc acttcgtacg atccgagcga
     1441 aaaggtcaga gaggccatga ccagggttgc cggagctgca tcgaggtttt cgacctccat
     1501 gtaaatttcg atgccggtct ggcccttcga aacgttggtg cgatcgcaga gaatcgtgtt
     1561 gattcgcacg gcggcttcga gtggcagaca gagcagcgaa acacagagaa gcactcgtcg
     1621 aagattgcga aaccatcgca tgattagact cctgacaggc aacccaaccg gatcagcaac
     1681 cgttggtaac gctgttgtgc cgacaggtcg gtgggtttgc aggtgagata cgtgcgcaag
     1741 acggaaccgg cggaagcggt ttgtccgtgc cggtccagta ttcttgacca acgggatgcc
     1801 gtgctgagga aataaaaacg atcatgaagc gctttgctga gatactcttc ccaggcttcg
     1861 tctgcttttc ccgcacgcag tttgtcttcg agcacacgga gagagcttct ggcagcgatc
     1921 tggttttcat gaattctcag agcgtcggca taacggggtt ctccgggagc tgcggcatgt
     1981 gcacgtcgca gccagtcgag tgctttggca tcactgccac gatcgatcag acgcgatgct
     2041 tcaatgaggc atgcttcagc aaccgccttc tcctgcggtg tcagagtgcg tgaaggaacc
     2101 acggatgctg gagccgttcc ttcgaaggtc ccggacaggg ctagcagagg aggctcggcg
     2161 g
//
LOCUS       DAORHD010000904         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63482_length_2160_cov_3.582898, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000904 DAORHD010000000
VERSION     DAORHD010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63482_length_2160_cov_3.582898"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="PKO06_16860"
     CDS             <1..422
                     /locus_tag="PKO06_16860"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV71376.1"
                     /translation="YEGATVITPNTREAEVALGEEFGCEADVLSGGMTLLQRFHTDAV
                     LITRGENGMSLFERGKPPMTIPTRAIDVYDVTGAGDTVIATFSMALAAGCCMQEAAEV
                     ANLAAGVVVGIVGTAVATPEMVLDHYDRIHYKDEVKR"
     gene            419..1480
                     /gene="rfaE2"
                     /locus_tag="PKO06_16865"
     CDS             419..1480
                     /gene="rfaE2"
                     /locus_tag="PKO06_16865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNV71377.1"
                     /translation="MRPDSLFHQDLTTLCRLVASDLDSLNERFSQALAITIDALRAGR
                     KVLAFGNGGSACDAEHLAAELVGRFGYDRASLPGIALTIPSATFSAIANDYGYEQVFA
                     RQIEGLAQSGDVAIGFSTSGKSPNVLRALQTARIRGVKTIVMTGAKDSPCAEAADVCL
                     RAPSASTPRIQEIHAVLVHSLCRGVEEALFPKPDRTSLPSDKVVNSARLSEFALAIKP
                     YDSVFTNGCFDILHPGHVTYLQQARRLGELLIVGVNSDDSVRRLKGPQRPYHRLEDRL
                     MMLSALQCIDYVVAFDEETPQKLIETLTPRVLVKGGDYSRDTVVGADWVEKHGGQVKI
                     VPLVEGHSTTRILEKTRHG"
     gene            1473..>2160
                     /gene="kdsB"
                     /locus_tag="PKO06_16870"
     CDS             1473..>2160
                     /gene="kdsB"
                     /locus_tag="PKO06_16870"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNV71378.1"
                     /translation="MAEQSRPAVQPIIVGVIPARLGSTRLPEKMLADICGEPLIVHTV
                     RAAVNSGVFERVLAATDHPRIVEVVKRAGYDALLTAADHPSGTSRVAEVAARIEGDVF
                     VNVQGDEPLIDAEGLRRLAEAFRQDPDLGMATLCFPLAAADEENPNAVKVVCDVRRNA
                     LYFSRSLIPHPRRREGFAPLKHIGVYGYRRETLMRLVQLPACPLEQIESLEQLRALYH
                     GIPIRLVDAAR"
BASE COUNT          398 a          611 c          702 g          449 t
ORIGIN      
        1 tgtatgaagg ggcaaccgtg attacgccca acacccgcga agccgaggtc gccctgggcg
       61 aggaattcgg ctgcgaagcc gacgtgctga gtggcggtat gactctgctg cagcgcttcc
      121 ataccgacgc cgtgctgatc acgcggggcg aaaacggcat gagcctcttt gaacgaggca
      181 agccccctat gacgattccg acccgtgcta tcgatgtcta cgatgtcacc ggtgccggtg
      241 acacggtgat tgccactttc agtatggctc tggcggctgg ttgctgcatg caggaggcgg
      301 ctgaagtggc gaacctggcc gccggggtgg tcgtcggaat cgtgggaacc gccgtcgcga
      361 ccccggaaat ggtactcgat cattacgatc gtatccatta caaagacgag gtgaagagat
      421 gaggcccgat tccctgttcc atcaggatct gacgacgctg tgccgcctgg tggcctctga
      481 tctcgatagc ttgaatgagc ggttttcgca ggctctggcc atcaccatcg acgcgctacg
      541 agccggccgg aaggttctgg cattcggaaa cggcggttcg gcgtgtgatg cggaacatct
      601 tgcggctgag ctggtcggcc ggttcgggta cgatcgcgcg tcattgccgg gaatcgctct
      661 gaccattccc agcgcgacat tttccgccat cgccaacgat tacggatacg aacaggtttt
      721 tgcccggcag atcgaagggt tggcccagtc gggggatgtc gcgatcggtt tttcgaccag
      781 cggcaagtcg ccgaatgtgc tgcgagctct gcaaaccgcg cgcattcgcg gcgtgaagac
      841 cattgtcatg accggagcca aggactcgcc gtgtgcggag gcggctgacg tctgtctgcg
      901 tgcgccttct gcgtcgacgc cccggattca ggagattcat gccgtgttgg tgcacagtct
      961 gtgccgaggg gtcgaggaag cattgttccc gaagcctgat cggacgagtc tgccatcgga
     1021 caaggtcgtt aatagtgccc gattgtccga gttcgctctg gcgatcaagc cttatgatag
     1081 cgtttttacc aatggctgct ttgacattct tcaccccgga cacgtcacct atctgcagca
     1141 ggcccgccgt ctcggtgagc tgctcatcgt cggtgtgaac tcggacgatt ccgtgcgccg
     1201 actgaaaggc ccccagaggc cgtaccatcg attggaagat cgtctgatga tgctgagcgc
     1261 gttgcagtgc attgactatg tcgtggcctt tgatgaagag acgccgcaga agctgatcga
     1321 aacgctgacc ccccgcgtgt tggtcaaggg aggcgactac tcgcgagaca ccgtcgtggg
     1381 tgctgactgg gtcgagaagc acggcggcca ggtgaaaatc gtccctctgg ttgaggggca
     1441 ttcgaccacg cggattctcg aaaagaccag acatggctga gcaatcacga cctgccgtac
     1501 agccgatcat cgtcggggtg atacccgcac ggctcggttc gacccgtctg ccggaaaaga
     1561 tgctggccga tatctgcggt gaacctctga tcgttcatac cgtgcgcgcc gccgtgaaca
     1621 gcggcgtttt cgagcgcgtg ctggcggcga cggatcaccc ccggatcgtc gaggttgtga
     1681 aacgggcagg atatgatgct ctgttgacgg cggccgatca cccgtcgggt acaagtcgtg
     1741 tggcagaggt tgccgctcgt atcgagggag acgtgtttgt gaatgtgcag ggcgatgagc
     1801 cgctcatcga tgccgagggg ctgcggagac tggccgaggc gttccggcag gatcccgatc
     1861 tggggatggc aaccctgtgt ttcccgctgg cagccgctga tgaagagaat ccgaatgcgg
     1921 tgaaagtggt gtgtgacgtc cgccgcaatg ccctgtactt ttcccgcagt ttgataccgc
     1981 acccgcgacg tcgtgaaggt ttcgcgccgc tgaagcacat cggggtctac ggctaccgcc
     2041 gcgagacgct gatgcgtctg gtgcagttgc cggcgtgccc gctcgagcag atcgagagtc
     2101 tcgagcagct gcgcgctctg tatcacggaa ttcccattcg cctggtggac gctgcccgag
//
LOCUS       DAORHD010000905         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63486_length_2160_cov_3.359145, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000905 DAORHD010000000
VERSION     DAORHD010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63486_length_2160_cov_3.359145"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PKO06_16875"
     CDS             complement(<1..169)
                     /locus_tag="PKO06_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005385975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HNV71379.1"
                     /translation="MFGKDFLLTWEKSTDELKQILAIANLLKGMREQNISARVFDSGL
                     AVSLFRDNSTRT"
     gene            complement(382..>2160)
                     /locus_tag="PKO06_16880"
     CDS             complement(382..>2160)
                     /locus_tag="PKO06_16880"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71380.1"
                     /translation="DLAAKGGRSFFHEGNTRMMMDFPPDTGAERRSADDRRHACRAHS
                     SQREGAACPFGQIARALLEDTDLPFRHRIRGALACLGRFVDAEQVFFWSLDPTAVGLH
                     DAIVWPKTLAGDSQWQHLPDIQAAPYLLERLQARPETIAAMLRGQDDRYREYLLWPVA
                     NRARLVAILGFAKAQNCGSWPQATRDLLQIAGPLFQIEWSRHLAERDKRRSELRYRVL
                     ASHLPNSLILLFDHDFRHLVAEGEAHLCDRLSASRIEGRTMRDIFSESECRLLEPLYR
                     RVLAGETVTQELAFGSEIFEVRFVPVRRVDGSVSSGMAVLQNITERKCIENELRQTQQ
                     ALQDHHEQETVLAAEIQRTLLQGTSVIRNRFIDLEIVSLPSMQVDGDFFDVFSGAEGV
                     YELIFGDVMGKGIPAAMIGAATRLAFLRALQTLPKTDAASVQAGPERILKLINQDITD
                     PLARLNSFITLLTAHLDAPRRLLKLVNCGHPPVIVYHLDGTLRLYASEYPPIGIMPPD
                     SYACREVVLRPGDLIIMVSDGVTEARNTAGEFFGEQRLLELIRASAGQPAAQVLHTIR
                     TTVFRFAGTRGLTDDFSCLVARIAGD"
BASE COUNT          477 a          644 c          614 g          425 t
ORIGIN      
        1 gggtccgagt ggagttgtcg cggaacagcg ataccgccag cccggaatca aacacgcggg
       61 cggaaatgtt ctgttcgcgc atacccttga gcaggttggc aatcgccagg atctgcttca
      121 gttcgtccgt ggatttttcc caggtgagca gaaaatcctt gccgaacatc ttggtcttgt
      181 agctcttgat ctctttcaac aacgcattga ggtttgccaa aggtcgtttc cccttcctgt
      241 ttctggtttt cgcgggctgt caaagtgtgt gtgcgcctga ccgcccgatc attcgccgca
      301 attgtatccg aaaacctgtc tgtccacaac ctgcaccccc ggcctgttac tgaccgctgt
      361 cgcggcgagg ccggaacgcc ttcagtcacc agcaatgcgg gcgaccagac aggaaaaatc
      421 gtcggtcagc ccacgcgtgc ccgcgaaccg aaagaccgtg gtacgaatgg tatgcagcac
      481 ctgcgcggcg ggctgtcctg cagaggcacg gatcaactcc agcaaacgct gttcaccaaa
      541 gaactctccg gccgtgttcc gcgcttccgt gaccccatcg gaaaccatga tgatcagatc
      601 gcccggtcgt agaaccacct cgcggcaggc gtaggaatcg ggcggcatga ttccgatcgg
      661 cggatactca gaagcgtaga gccgcagcgt tccatccaga tggtacacta tcacgggcgg
      721 atgtccacag ttcacgagtt taagcagtcg cctgggtgca tccaggtgag ccgtcagcaa
      781 ggtgatgaag gaattcagac gggccagggg atcggtgatg tcctggttga tcagtttcag
      841 aattcgctcc ggaccggcct ggacgctagc ggcatccgtc tttggcaacg tctgcaacgc
      901 acgcaaaaat gcgagtcggg tcgcggcacc gatcatggcg gccggaatcc ctttgcccat
      961 gacatcgccg aagatcagtt catacacccc ttccgcaccg gaaaacacgt cgaagaagtc
     1021 accatcgacc tgcatcgaag gcagggaaac gatttccaaa tcgatgaaac gattacgaat
     1081 cacggacgtt ccctgcagaa gcgtacgttg aatctcggca gccaggactg tttcctgttc
     1141 gtggtgatcc tgcagagcct gctgcgtctg gcgcagttca ttctcgatgc acttgcgttc
     1201 ggtgatgttc tgcagaaccg ccattcccga tgagacggaa ccatcaaccc gacgcaccgg
     1261 cacgaaacgc acttcgaaaa tctccgaccc gaacgccagt tcctgtgtca cggtctcccc
     1321 cgccaatacc cgtcgataca agggttcgag aagacggcac tcggactctg agaagatgtc
     1381 acgcatggtt cgtccctcga ttcgtgaggc agaaagacgg tcacaaagat gggcctctcc
     1441 ctccgccacg aggtgacgaa agtcgtgatc gaagagcaga atcagactgt tcggcagatg
     1501 gctggccaga acccgatatc gaagctcgct gcgccgtttg tcccgttcag ccagatgacg
     1561 agaccattcg atctggaaaa gtggtccggc gatctggaga agatctcgcg tcgcctgtgg
     1621 ccaactgccg cagttctgcg ctttcgcgaa gccgagaatg gccaccagac gggctcggtt
     1681 ggcaaccggc cagagtagat attcgcggta ccgatcatcc tgtccgcgaa gcatcgcggc
     1741 tatggtttcg ggcctggctt gcagacgttc gagcagatac ggtgccgcct gaatgtccgg
     1801 cagatgctgc cactgactgt caccggcaag cgtcttcggc cagacgatgg cgtcgtgaag
     1861 accgacggcc gtcgggtcga gagaccagaa aaacacctgt tcagcgtcga cgaatcggcc
     1921 cagacaagcc agggctccgc ggatgcgatg tcgaaaaggc agatccgtgt cttcgagcaa
     1981 agcgcgggcg atctgcccaa atgggcaagc agcaccttcg cgctgagagg aatgcgcccg
     2041 acacgcgtgg cgccgatcat cagcagaccg gcgttctgcg cccgtatcgg gcggaaaatc
     2101 catcatcata cgcgtgttcc cctcatgaaa aaagcttctt cctcccttcg cagcaagatc
//
LOCUS       DAORHD010000906         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63515_length_2159_cov_3.836502, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000906 DAORHD010000000
VERSION     DAORHD010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63515_length_2159_cov_3.836502"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PKO06_16885"
     CDS             complement(<1..679)
                     /locus_tag="PKO06_16885"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV71381.1"
                     /translation="MNSTSHIFSRRVACLAMMAVLVLCLSATAHAEDNISLDFRDADI
                     RDILKIIAQKSGTNIIAEKNVRGNVTVRLNDVYYEEAMNLIAKTNGFAVRKIGNTWVL
                     ADEKKLVESFEKGLTITKRLQYAKAADVSKVVQSVIKKDIKVATDDRINAVIVSGGKD
                     ILDEVKKLIETVDTPVHQVMIESKIVEVRTNASRDLGFNWALGANGQTGPLAGSEGRQ
                     PIFVTREY"
     gene            complement(676..1380)
                     /locus_tag="PKO06_16890"
     CDS             complement(676..1380)
                     /locus_tag="PKO06_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71382.1"
                     /translation="MNRKTDGRVRHALFLICVLCLFFMGLASPTQAQEPDPFAIPEGG
                     DKPAAEGGDKPAEGGDKPAGEGGDPMPANPAEDAGAPPAESGDKPSGDSSGGDSAPAE
                     PAKSLDENEQIELIIQNHKFKLENELKDPFKPLIEKKVAVPVVVAPVNKNSGPPKPPP
                     PPPPKPIQLFVQGVCGNENERLAMIVFENKNYTIQKDQVVDGKFKVVDILTDKLIIYS
                     NKEQMRRTFPIGGGKE"
     gene            complement(1427..1981)
                     /gene="pilO"
                     /locus_tag="PKO06_16895"
     CDS             complement(1427..1981)
                     /gene="pilO"
                     /locus_tag="PKO06_16895"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNV71383.1"
                     /translation="MNNFGVAFFLLIVAAACAGGAFYVYYWEPAQVDREKLITEIKGL
                     EARQAEIQNLHDEIAKIKEDIAKKETEKNKLAMESNQLNTVVPKLLDSTEAVAAKFDV
                     KFQDIRISPLVKAEQWSELPVEMSVLGTYSAVANFLHVMEKRKIINLAAGSVNISVSA
                     ETDPKTKSPLLTVTLNGKVYIMGG"
BASE COUNT          428 a          609 c          585 g          537 t
ORIGIN      
        1 agtactcgcg ggtcacgaag atcggctggc ggccttcgga accagcgaga ggaccagtct
       61 ggccattggc gccaagtgcc cagttgaaac ccagatcgcg actggcgttg gtgcgcactt
      121 cgacgatctt cgactcgatc atgacctggt gaaccggggt gtcgacggtt tcgatgagct
      181 tcttcacctc atcaagaata tccttgccac cagacacgat gacagcgttg atgcggtcat
      241 cggtggcgac cttgatatcc ttcttgataa ccgactggac gaccttggac acgtcagcag
      301 cttttgcata ctgcaagcgc ttggtgatag tcagaccttt ttcgaaggat tccacgagtt
      361 tcttttcatc agccagaacc caggtgttgc cgatcttgcg aacggcaaac ccgttggtct
      421 tggcgatcag gttcatggct tcttcgtagt agacgtcatt gaggcgcacc gtgacgttgc
      481 ctcggacgtt cttctcggca atgatgttgg taccggactt ctgagcgata atcttcagaa
      541 tgtcgcggat gtcggcatca cggaagtcaa gagagatatt gtcttccgca tgtgccgtcg
      601 cggacaggca caggaccagg acagccatca tcgccaggca ggcaacgcgg cgcgagaaaa
      661 tgtgactcgt ggagttcatt cttttccacc tccgatcgga aatgtccgac gcatctgttc
      721 cttgttcgaa taaatgatca gtttgtcagt taagatatcg acaactttga actttccatc
      781 cactacctga tctttctgga tcgtgtagtt tttgttctcg aagacaatca tggccaggcg
      841 ctcgttctcg ttcccgcaca caccctgaac gaagagctga atcggcttgg gcggcggcgg
      901 gggcggcggc ttcggaggcc ccgaattctt gttcaccggt gcgacgacga ccggcaccgc
      961 gacctttttc tcgatcagcg gcttgaacgg gtctttcaac tcattctcaa gcttgaattt
     1021 gtgattctgg ataatgagtt cgatctgctc attttcatcg aggctctttg ccggttcggc
     1081 gggcgcgctg tcgccaccgg aactgtcgcc ggacggcttg tcgcctgact cggccggcgg
     1141 tgctcccgca tcctcggccg ggttggccgg catcggatcg ccaccctcgc ctgccggctt
     1201 gtcgccacct tcagcaggct tgtccccgcc ctcggcagca ggcttgtcgc cgccctcagg
     1261 gatcgcgaag ggatcaggct cctgggcttg tgttggtgac gccagcccca tgaagaacag
     1321 gcagagaacg cagatcagga acaaggcatg ccgtacccga ccatcggttt tcctgttcat
     1381 attccacctc cgtgaaatga cgtgtgccat catgttgttc actccctcag ccgcccatga
     1441 tgtagacctt accgttcagg gtcacggtaa gcagggggct cttggtcttc ggatccgttt
     1501 ccgccgaaac agagatgttc acggaaccag ccgccaggtt gatgatcttg cgtttttcca
     1561 tgacatgcag gaagttcgcg accgcagaat acgtgcccag aacggacatt tcgacgggca
     1621 gttcagacca ctgttcggcc ttcaccagcg gcgagatgcg aatatcctga aacttcacat
     1681 cgaacttggc tgccacggct tcagtcgagt cgagcagctt gggcacaacg gtattcagct
     1741 gattcgattc cattgccagt ttgtttttct cggtctcctt cttggcaatg tcctctttga
     1801 tcttggcgat ctcgtcatgc aggttctgaa tttctgcctg acgcgcctcc aggcccttga
     1861 tctccgtgat caatttctcg cgatcaacct gagccggttc ccagtagtag acgtagaagg
     1921 ctccgcccgc gcaggccgca gccacaatta acaggaagaa tgcgacacca aagttgttca
     1981 tgggacctcc tgctcagttc tcgccacggc tgatgtcacg tttcaagcgg cagttgacat
     2041 cgaagcgcca gacgggagac ttttcgtagg tgtttttgtt cgcgttgctc aggaacacat
     2101 cttcgaagta agagtgatcc tgaatctgtt tcaagaggtt ctggataccc tgtgtcagg
//
LOCUS       DAORHD010000907         2159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63534_length_2159_cov_2.978612, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000907 DAORHD010000000
VERSION     DAORHD010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63534_length_2159_cov_2.978612"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKO06_16900"
     CDS             <1..596
                     /locus_tag="PKO06_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methylamine utilization protein"
                     /protein_id="HNV71384.1"
                     /translation="SAEQLQLTVVDTSEKPVADAVVVIRGDGLAPLPATGPALITQHD
                     KTFRPLVTVIRIGTAVEFPNEDDIMHHVYSFSKPKKFDIPLYKDKKPEPVIFEQPGVV
                     TLGCNIHDWMKAFIYVTDAPVFGKTSETGTLAFTVPVGTCAIDVWHPRLKKVQGDPLP
                     RQVTIIRGAPSDLRIVLELKKSSTPSRAPTLDTGGSY"
     gene            676..>2159
                     /locus_tag="PKO06_16905"
     CDS             676..>2159
                     /locus_tag="PKO06_16905"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71385.1"
                     /translation="MTFRFRTFKARLLTIFLSLFVLVGIAAFLLVHRVSEQYAREQAE
                     RSLEVSGRVLSETIRSRGELLRQAVYMLSRDFAFQQAFATDDLATVRSAMLSLKSRLK
                     ADLMLLSHPEEPFATVVETENLVEAGTPFPGSDMIHAALESEKPVSGILELEGRLFIT
                     IVVPLLAPEPIAWFVVGFQIDDTFVRELQTITNTDLSVIVEGSGTARLHASTLSETQR
                     ASLMQVYASFNHELEVSKLQLGGDPWLLRSIRVGHTRMIRLILGKSLSRAMAPFYQLR
                     MLLFSIALIGLVITIIGVFLVARHVTVPLRALVDKTRLIEEGTYDQPIVVTQEDEIGD
                     LARAYNHMSKGLADRDKVRALLGKVVSGAIADELLKSGEVQLGGEEKEVTILFSDIRN
                     FTTLCEGRAPSQILEMLNDYLTRMNQVIEAHGGVVDKFIGDAIMALFGAPVPHPDDVD
                     RALRAALQMKDELAQINVELAKKNLPQVSIGIGINTGVVVAGNMG"
BASE COUNT          442 a          661 c          583 g          473 t
ORIGIN      
        1 tctctgctga acagctccaa ctgaccgtgg tcgacacatc cgaaaaaccc gttgccgatg
       61 cggtggtcgt gatacgcggg gacggactcg ctcccttgcc cgccactggc ccggcgctca
      121 tcacgcaaca tgacaagacg tttcggccgc tcgtcacggt catccgaatc ggcacggccg
      181 ttgagtttcc gaatgaagac gacatcatgc accacgtgta ttctttctcc aaaccgaaga
      241 agttcgacat tcctttgtac aaagacaaga aacccgaacc ggtcatcttt gagcaacctg
      301 gcgtcgtcac tctcggctgc aacattcacg actggatgaa agccttcatc tatgtcaccg
      361 atgcccccgt gttcggcaag acttccgaaa ccggaaccct ggccttcacc gttcccgtcg
      421 gcacctgcgc catcgacgtc tggcaccccc gtctgaagaa ggttcaggga gacccgcttc
      481 cccggcaggt caccatcatt cgcggcgctc cttcagatct tcgcattgtc ctggaactga
      541 aaaagagttc gactccgtca cgtgccccca ctctcgacac cggtggaagc tattaaccga
      601 gcgactccgc gtcaggtcgc ccgtgagcgt ccgggctggg tatggctgat gtcggcacag
      661 gaagaggcat agcgcgtgac gtttcgattt cgtaccttca aagcccgtct gttgactatt
      721 tttctctcgc tgtttgtcct ggtcggcatc gctgcatttc tgctggtaca ccgggtttct
      781 gaacaatatg cccgcgaaca ggccgaacgc agcctggaag tcagcggtcg cgtgctgagc
      841 gagacgattc gaagtcgcgg cgaacttctg cggcaggcag tctacatgct gtcacgcgac
      901 ttcgcctttc agcaggcttt tgccaccgac gatctcgcga ccgtgcgttc ggccatgctg
      961 agcctgaaat cacgtctaaa agcggacctg atgctgttat cccacccgga ggagcctttt
     1021 gccacggtcg tggaaacaga aaacctcgtc gaggcaggta ctccatttcc gggttccgac
     1081 atgatccacg ccgcgctcga gagcgagaag ccggtcagcg gcattctgga actcgaaggt
     1141 cgactgttca ttaccatcgt ggtgccactt ctggctcccg agccgattgc ctggtttgtt
     1201 gtcgggtttc agattgatga cacgttcgtg cgagagttgc agacaattac caatacagat
     1261 ctgtccgtca ttgtcgaagg ctcaggaacc gctcgtctgc acgcgtccac cctttccgaa
     1321 acacagcggg cttctttgat gcaggtctat gccagtttca accatgaact ggaagtttcg
     1381 aaactgcagc tcggcggcga tccgtggctg ttgcgatcga ttcgtgtggg tcacacccgc
     1441 atgattcgac tgatcctggg caagtctctg tccagagcga tggcgccatt ctaccaactg
     1501 cgaatgcttc ttttttcgat cgctctcatc ggcttggtca tcacgatcat tggtgtgttt
     1561 ctcgtcgccc gccatgtcac cgtgccgctg cgcgctctgg tcgacaaaac ccgcctgatc
     1621 gaggaaggca cctatgatca gccgatcgtc gtcacgcagg aagacgagat cggggatctt
     1681 gccagggcct acaaccacat gtcgaaggga ctcgccgatc gtgacaaagt gcgcgctctc
     1741 ctgggcaagg tcgtgtcggg ggccattgcc gatgagctgc tgaaatccgg cgaagtacaa
     1801 ctcggcggcg aagagaagga agtgacgatc ctgttttccg acattcgcaa cttcaccacc
     1861 ctctgcgaag gccgagcccc ctcccagatt ctcgagatgc tcaacgatta cctgacccgc
     1921 atgaatcagg taatcgaagc tcatggcggc gtcgtcgata aattcatcgg cgacgccatc
     1981 atggccctgt tcggtgcccc cgtgccccac cccgatgacg tcgatcgcgc cctgcgtgca
     2041 gccctgcaga tgaaagacga actggcgcag atcaacgtcg aactggcgaa aaagaatctg
     2101 ccgcaggtct ccatcggtat cggtatcaac accggcgtcg tggtggccgg caacatggg
//
LOCUS       DAORHD010000908         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63558_length_2158_cov_3.777461, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000908 DAORHD010000000
VERSION     DAORHD010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63558_length_2158_cov_3.777461"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2158)
                     /locus_tag="PKO06_16910"
     CDS             complement(<1..>2158)
                     /locus_tag="PKO06_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV71386.1"
                     /translation="MNGKRAVGVIVQKQSGANSVTVSNAILEALPSIQSQLPADVKVS
                     VFFDTSEFIRNTIKNLSDSVLIGGIAVFAVIFFFLRDLVASLIVCTTIPTSLIITFLL
                     MYLAGYTINQITLSSLAIAIGMVVDNAIVILDNIKRYLDRRVSSRESAIWGAVEMGAS
                     VTASTLTTIVIFLPIIFTRGITQIFFGQLAMIVIMALTASLFSAMMLTPMLCSRFLKP
                     ASKSPGNTPDRGQRFLLWLEEQYGTFLNRVLNHRLKLVAALVGMLILSIGLVRLVGVD
                     FIPEQDQGRLVIRYEMQTGTRYELTGEVGKRILGIINERVPELRASVLRFGRSADSIG
                     AVITGSKEGSHTGQVELRLTRKDERKRGLKQVIEDIRPEIEKIPGIVVRFDAGDPLAN
                     MMGTGGAGFTLNVYGYDLKIGLEYAQELVKGLSAVRGLKDLEISQQLAQPELQVIVDR
                     EKASNLGLNVTDVGKTVELCFSGDDTVKYREGGDEYDIEVRLREADRISFDDLAQVPL
                     QSPTGQTVRVENVARIRQDFGPTRITRNEQERYIQITGQVYGRGSGDIAQDAEKVINA
                     VPLPPGFSWKFAGNEKDRRESFLLMLGAAALGMVLVYMVMASQFESLLAPFIIFLSIP
                     FGFMGAIQILALTGHRISIISLLGFIILIGIVVNNGIVLVSYINILVQRRYALREALI
                     QAGKSRLRPVLSTTLTTILGMAPMAMSKGEGSEIWVP"
BASE COUNT          499 a          589 c          610 g          460 t
ORIGIN      
        1 cggaacccag atctcggagc cttcgccctt ggacattgcc atcggggcca tgccgagaat
       61 cgtggtcagc gtggtcgaaa gaacggggcg cagacggctt tttccggcct gaatcaaggc
      121 ttcgcgcagg gcatatctgc gctggaccag aatattgata tagctgacca gcacgatgcc
      181 gttgttgacc acgatgccga tcagaatgat gaatccgagc agtgagatga tcgagattcg
      241 atggccggtc agagcgagaa tttgaatggc acccatgaac ccgaacggaa tactcagaaa
      301 gatgatgaac ggtgccagca gagactcgaa ctgcgaggcc atgaccatgt agaccagcac
      361 catgccaagc gcggccgcac ccagcatcag gagaaacgat tcgcggcggt ctttctcgtt
      421 cccggcaaac ttccaggaga atccaggtgg caagggaacg gcgttgatca ccttttcggc
      481 atcctgtgcg atgtcgccgg aacctcgacc gtacacctga cccgtgattt gaatgtatcg
      541 ttcctgttcg ttgcgggtaa tgcgcgtcgg gccgaaatcc tgcctgatcc gggcgacatt
      601 ctcgactcga accgtttgtc cggttgggct ttgcaggggc acttgagcca aatcgtcaaa
      661 gctgatgcga tccgcttccc gcagtcgcac ttcgatgtcg tactcgtctc ccccctcacg
      721 gtatttcacc gtgtcgtcac cactgaagca gagttccacg gtcttgccga catcggtgac
      781 gttcagccca aggtttgaag ctttttctcg atcgacgatg acctgcagct caggttgggc
      841 gagctgttgg ctgatctcaa gatccttcag accgcgcacg gcagaaagtc ccttgaccag
      901 ttcttgtgca tactcgagac cgatcttcag gtcataccca tacacgttga gcgtaaaccc
      961 tgcaccgcct gttcccatca tgttggcaag agggtcgcca gcatcgaaac gcacgacgat
     1021 tccgggaatt ttttcgatct cgggtctgat gtcttcaatg acctgtttga gtccgcgctt
     1081 tcgctcatct ttgcgagtga gtcgtaactc tacctgaccg gtgtgcgacc cttctttcga
     1141 accggtgatg acggccccga tactgtcggc gctgcgcccg aagcgcagta ccgatgccct
     1201 caactcaggc acgcgttcgt tgatgatgcc gagaatccgt ttgccgacct ctccggtgag
     1261 ctcataacga gtgcctgtct gcatctcata ccgaatgacc agacgtccct ggtcctgctc
     1321 cggaatgaaa tccacgccga ccaggcgtac caacccgatg gaaagaatca acataccgac
     1381 gagagcagca accagtttga gtctatggtt cagcacccgg ttgagaaaag tgccgtattg
     1441 ctcttcgagc cagagcagga atcgctgccc gcggtctggt gtgttgccgg gtgacttgga
     1501 ggcgggtttc agaaaacgcg aacacagcat cggtgtgagc atcatcgccg agaaaagcga
     1561 ggcggtcaga gccatgatga cgatcatggc cagttgcccg aagaagatct gcgtaatgcc
     1621 gcgcgtgaag atgatcggca gaaagatgac gatggtggtc agtgttgatg ccgtgaccga
     1681 agctcccatt tcaacggcgc cccagatcgc gctttcccga ctcgacacgc gccggtccag
     1741 atatcgtttg atgttatcga gaatgacgat cgcgttgtcg acgaccatgc cgatggcgat
     1801 cgccagactc gagagcgtga tctggttgat ggtgtagccc gcaagataca tcaggagaaa
     1861 ggtgatgatg agcgaggtcg gaattgtcgt gcagacgatg aggctggcaa ccagatcgcg
     1921 gagaaaaaag aaaatgaccg cgaagacggc aataccgcca atgagaacag aatcagaaag
     1981 gttcttgatg gtattgcgaa tgaactctga cgtgtcgaaa aagaccgaga ccttgacgtc
     2041 agccggcaac tgcgactgaa tagatggcag agcttcgaga atagcgttcg agacggttac
     2101 cgaattggct ccggactgct tctggacaat gactccgacc gcgcgtttcc cattcatg
//
LOCUS       DAORHD010000909         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63569_length_2158_cov_2.682359, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000909 DAORHD010000000
VERSION     DAORHD010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63569_length_2158_cov_2.682359"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..773
                     /locus_tag="PKO06_16915"
     CDS             54..773
                     /locus_tag="PKO06_16915"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HNV71387.1"
                     /translation="MDFSTNRDYLNGSRQETVSRPGGAWTGMSEQAFMTRVLPYFGAG
                     LLITALGGHLSLRLFAQIFSMPLLLGLSVLNLIMYFVLMWQRFTPGLNVTLYYLYTFI
                     NGLLLGPLLMAARISGGNILIVQALGITALAFFAITAWVRFTGKDFSGLRPFLVTGLM
                     VVIGAMIVNMFVGGSGLYLGISILSAFLFMGFIAYDMSNIMLKYREEEFILATIQLYL
                     DFLNLFIAVLRILISLSNRRD"
     gene            896..1096
                     /locus_tag="PKO06_16920"
     CDS             896..1096
                     /locus_tag="PKO06_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71388.1"
                     /translation="MQLETIVVGFVSGIISGILLLAGTWVFLLPKVPTLLRREAVRDM
                     SHYGRDVGKKGVGYLLKLLLHR"
     gene            1214..>2158
                     /locus_tag="PKO06_16925"
     CDS             1214..>2158
                     /locus_tag="PKO06_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71389.1"
                     /translation="MPIEKPRSLEDRILFFLQSKNLPDGLELFYQNFNAEHPPTTEFF
                     GKLIDGLRALRAREEAYEALQRVSSWIPDAQALKKLQGDLQREYYDALVDKGKKQFDQ
                     TTQRSKEFSEHTLTKVDVLAREKLEEENRNTLRLLYQHALDDFKRALDIQPAGLAALN
                     GAFRCYLALGDSVKAQEIESRILELTQLEDKKKQEESKRLEEQIRAKEAAKAPPPDEV
                     IVDLPTLKRWYEQQRHQDVLRGLKKYLAKHPAVIAGYLLGARSHLELHEFHQAEMYLE
                     RALKIDPYFEETVQFKLDYLEKKYLILSRAATEYMNKGI"
BASE COUNT          500 a          597 c          524 g          537 t
ORIGIN      
        1 gtcttccgcg tatgataaga taccatgtga acgacaagat gcaggagggt ttcatggact
       61 tctcaacgaa ccgcgactat ctcaacggat cacgccagga gacggtttct cgtcccggtg
      121 gagcctggac aggcatgtcg gaacaggcat tcatgacgcg ggttctgccc tactttgggg
      181 ccggattgct gatcaccgct ctcggcggac atctttccct acgcttgttt gctcagatct
      241 tcagtatgcc gctccttctc gggctgagtg tattgaatct gatcatgtat ttcgttctca
      301 tgtggcagag attcactccg ggcctcaatg tgaccttata ctatctgtac accttcatca
      361 acgggctgtt actcgggcct ttgctgatgg cagcccgcat ttctggcggg aacattctga
      421 tagtgcaggc tctgggcatt accgcgctcg ccttctttgc cattaccgcg tgggtgcgtt
      481 tcaccgggaa ggacttttcc ggtctgcggc ccttcctggt gactggtctc atggttgtca
      541 ttggcgccat gatcgtgaat atgttcgtcg gcggcagcgg actctatctc ggaatctcga
      601 ttttgagcgc tttcctgttc atggggttca tcgcgtacga tatgtccaac atcatgctga
      661 aatatcgcga agaagaattc attctcgcga cgattcaact ctatcttgat ttcttgaacc
      721 tgttcatcgc agtactccgc attctcatct ccctgtccaa ccgacgcgac tgacccgcac
      781 cttctcgtat caccgcaagg ggcgcacctt tcaaggtgtg cctctttttc attaaccaaa
      841 cctcgattcc tgccgacaat accctcaccg atcgcgttgg aagggagaaa tcgagatgca
      901 acttgaaacg attgtggtgg ggtttgtttc cggaatcatt tcgggaattc ttcttctggc
      961 cggaacgtgg gtctttcttc tgccgaaagt accaactctt ctgcgccggg aagcagttcg
     1021 cgacatgagt cactacggta gagatgtcgg aaaaaaggga gttggttatc ttctgaaact
     1081 gctactccac cgctagcact tccgtttcat cgcgtccgat aacgccggca gcttgctgcc
     1141 ggccttttct ctttccgttg aattcttgat tttctgagcc tttcagacta tagtagcagg
     1201 gtttccacca tttatgccga tagaaaagcc ccgcagtctc gaagatcgca tcctcttttt
     1261 cctccagagt aaaaacctcc ctgacgggct cgagcttttc taccagaatt tcaacgccga
     1321 acatcctcca accactgaat ttttcgggaa actcattgac ggactgcggg ctctgcgagc
     1381 acgagaagaa gcatatgaag cgctgcagcg agtttccagc tggattcccg atgctcaggc
     1441 gttaaagaaa ttgcagggtg acctgcaacg cgaatactat gatgcgctcg tcgacaaagg
     1501 gaagaaacag ttcgatcaaa cgacgcaacg ctccaaggaa ttcagcgagc acaccctgac
     1561 caaagtcgac gtgctcgcac gcgagaaact cgaagaggaa aatcgcaaca ccctacgcct
     1621 tctctatcag catgctctcg acgatttcaa acgtgcgctc gatatccagc cagccggact
     1681 cgcggccctg aacggcgctt tccgctgcta tctggccctc ggagattcgg tcaaagccca
     1741 ggaaatcgaa tcgcgcattc ttgaactcac ccagcttgag gacaagaaaa aacaggaaga
     1801 atcaaaacgt ctcgaggaac agatacgcgc caaggaagcg gcgaaagcac caccgccaga
     1861 cgaagtcatc gtcgaccttc cgacactcaa gcgctggtac gaacaacagc gacatcagga
     1921 tgttctccgc ggtctgaaga aatatctcgc caagcatccg gccgttatcg cgggttatct
     1981 tctcggtgcc cgatctcatc tcgagctgca tgagtttcat caggctgaaa tgtatctcga
     2041 acgagctctc aagatcgatc cctactttga agaaacggtt cagttcaaac ttgattacct
     2101 ggaaaagaag tatctgatct tgagccgcgc cgcgaccgag tatatgaaca agggcatt
//
LOCUS       DAORHD010000910         2158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63571_length_2158_cov_2.490728, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000910 DAORHD010000000
VERSION     DAORHD010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63571_length_2158_cov_2.490728"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PKO06_16930"
     CDS             complement(<1..1235)
                     /locus_tag="PKO06_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71390.1"
                     /translation="MIQLIREALKANRTREKVLLCAGNAYGRLLQRELLKLEPGLVNL
                     SFTSVADYVGRTMAFMLMDKGIRVLKPGHGMLILQELIDRDGYFREAVDMPGFLIALW
                     NCMLDLRRSGLKPEQLTAEIVGNERKAQELQILMKKFEAYLADHHLIDYAGILKLGIF
                     ERRLEQSADLILLPGHLPLEPLERQFLKQAENRLLHLPYHVPNSLAIPDSWHRECGRE
                     SPRIAQASIPADCNISLFAATEPQAEIREIIRRIRASNRPFEEMVIGLAQPAEYLSLL
                     TSHLEIAGIPYVSAIAPEMNDSRPGRLALSLCDWALNGFPATGLIAMLNQGDLRLFRP
                     RRRRGDDGVAKAVISSQKAIHLLREGHVRGGRDGYEKPLLAVGDSANDRGHPEIRRQV
                     RRLSRRIGELMAKFPETGTP"
     gene            1385..1591
                     /locus_tag="PKO06_16935"
     CDS             1385..1591
                     /locus_tag="PKO06_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71391.1"
                     /translation="MKANIRSRTWWDAIYYDGTSPLEDLIDDTDIFPIDDPEEEWSFC
                     VSPSEFAKALGISLKKLNRLFREE"
     gene            complement(1615..>2158)
                     /locus_tag="PKO06_16940"
     CDS             complement(1615..>2158)
                     /locus_tag="PKO06_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71392.1"
                     /translation="REIMREQLMNWDKKTVTTRLPLLVREYESEAPRFSMVFVEFEHD
                     TEKFPVGIYTYGTHKGLAFCSQEEPTGEAGHTARMQFGYLLSFKRLIDKARLDALYDE
                     MLEKFEKKPMPRSTLFGMACKLAVGRDLLVVAAESSLDWYRKAAKKKGLSIATLSKEA
                     MGDRPFKQIQSFNIYYHLYE"
BASE COUNT          458 a          634 c          574 g          492 t
ORIGIN      
        1 ggcgttcccg tttccggaaa cttcgccata agctcgccga tgcgcctgga cagtctgcgc
       61 acctggcgcc gaatctccgg gtgccctcga tcgttcgcgc tgtcgccgac cgcaagaagc
      121 ggcttttcat acccatcccg gccgccgcga acatggcctt ccctgagcag gtgaatggct
      181 ttctgcgaac tgatcaccgc cttcgcaacc ccgtcgtctc cacgacggcg tctcgggcga
      241 aaaagtcgca ggtcaccctg gttgagcatg gcaatcaaac ccgttgccgg aaagccgttc
      301 agagcccagt cacagagact cagcgccagc cgtccagggc gagaatcgtt catctcgggc
      361 gcgatggcgc tgacatacgg aatcccggcg atctcgagat gtgaagtcag caggctgaga
      421 tactccgcag gctgtgccag gccgatgacc atctcttcaa aaggccggtt cgaggcacga
      481 atgcggcgta tgatctctcg tatctcggcc tgtggttccg tcgcggcgaa cagagaaatg
      541 ttgcagtcgg cagggatcga ggcctgagcg atgcgaggtg attctcttcc gcactcacga
      601 tgccagctgt cgggtatcgc gagagagttc ggtacatggt acggcaggtg cagaagcctg
      661 ttttcagctt gtttcaggaa ctgccgttcg agaggctcga gcggcagatg ccccggcaat
      721 agaatcagat cggcgctctg ctcgaggcga cgctcgaaga tgccgagctt caagatgccc
      781 gcgtaatcga tgagatgatg gtcggcgaga tacgcttcaa actttttcat gagtatctga
      841 agctcttgag ccttgcgctc attgccgaca atttctgccg taagctgttc aggcttcagt
      901 ccagatcttc gaagatccag catacagttc cagagcgcga tgaggaaacc cggcatatcg
      961 accgcctcgc ggaaataccc gtcgcggtcg atcagctctt gcagtataag catgccgtgc
     1021 cctggcttga gcactcgtat tcccttatcc atgagcatga aagccatcgt gcggccgacg
     1081 tagtccgcaa ccgacgtgaa agacagattc accaacccag gttcgagctt taacagctca
     1141 cgttgcagaa gtctgccata ggcgttccct gcgcataaaa gaactttctc gcgggtccgg
     1201 ttggctttca gtgcctctcg aatcagttga atcacgacgt ttcccccctc atgtcagttg
     1261 cctgcccatc ttacctcaga cacttccgtt gcgcatcttc actgctttca tgtcgcgcgg
     1321 cctcctttcc ggaatgtgtt gttcggaaag ctctcaaaac ctcgcgaaaa aacaggaggc
     1381 agggatgaaa gcgaatatcc gttcaagaac ctggtgggat gcgatctact acgacggcac
     1441 ttctccactc gaggacctga tcgacgatac agacatcttt cccattgatg accctgaaga
     1501 ggaatggtcg ttctgcgttt cgccttctga gtttgcgaaa gctctcggca tttcgctgaa
     1561 gaagctcaac agactctttc gcgaagagta atgctactga cgattcagcg tcacctactc
     1621 gtacaaatga tagtagatgt tgaacgactg aatttgtttg aatggcctgt ctcccatcgc
     1681 ctccttggac agcgtggcga tcgacaggcc ctttttcttt gccgcttttc tgtaccagtc
     1741 gagagacgac tcggcggcca ccaccagcag gtcgcggccg acggcaagct tgcacgccat
     1801 gccgaacagc gtactgcgcg gcatcggctt cttctcaaac ttttcgagca tctcatcgta
     1861 caacgcatcg agacgggcct tgtcgatgag gcgcttgaat gacagcagat agccgaactg
     1921 cattcgggcc gtgtgccccg cctcacctgt cggctcctct tgagagcaga aagccagccc
     1981 cttatgcgtt ccgtaggtgt aaatgccgac cggaaacttc tcggtatcat gctcaaactc
     2041 gacgaacacc atgctgaacc gcggtgcttc gctttcatac tcgcgcacaa gaagaggcag
     2101 acgtgttgtg actgtctttt tgtcccagtt catcagttgc tctcgcatga tctcgcgc
//
LOCUS       DAORHD010000911         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63731_length_2155_cov_2.094762, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000911 DAORHD010000000
VERSION     DAORHD010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63731_length_2155_cov_2.094762"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2155)
                     /locus_tag="PKO06_16945"
     CDS             complement(<1..>2155)
                     /locus_tag="PKO06_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71393.1"
                     /translation="GMSAWADIEADVVATLAGLIGDVSPLFGTVAGRTVMDRKSTVAA
                     ILRDKVTGRPRGGVLLTIPETRIAPSWVFERARRERRDAAVLRRWAPSSPTRPERTWL
                     PTVGGLGWVDVARHHPEIHLLAYVPVRLLAVPARRYLPWVDLALLLFFLFSGLVLWRW
                     HLQEKPVALRIYGQLNLALTISALLPLIGFTLVAWAYASFHYSFEREQLKKRLEWRLA
                     AIDQRYRSFMERQLGKFRELRDWAGQHFSDPETVRQRLDVLSTQREIQGFFIITTEGK
                     LIRNDTKEDRLLQSIFKGVAFKLFKALRLETPGAKSVADLAEAAMGDDLFKLLMAQSL
                     AEFMVSLDRFFYLATGDREPMTFFIDILYRDLERREVAGVIVLMQSVYNSLPRFIDQE
                     LFRETDPLRTMGVNTLKVVRREGNTLKVRPAGTSLSARELDAHALAADVREAVVRDYF
                     DSSDPWVIGARRISSNQYVVVAGLDGRPLLRSHHVIVWGLYLLVAYALLVLVGVTRFL
                     SGRFAQPLLELRAAADAVSGRDFSVRVALDSKDEFGELGEAFNRMTRGLYEREQMSRF
                     VSGDVLEAVRQDNATEHAERAEVTILFSDIRGFTSLSERFEPEVIVTMLNDYFTVMEA
                     CIVEQGGTIDKYVGDAIMAVFRERQGMPPHWWRAARAALDMRDALLRFNRDRQQQGLF
                     TVANGIGINTGEVISGRVGSRTGRLDFTVIGDAVNL"
BASE COUNT          490 a          678 c          604 g          383 t
ORIGIN      
        1 cagattcacg gcatcgccaa tgacggtgaa gtcgagacgc ccggtgcgtg acccgacacg
       61 accggaaatc acttcaccgg tattgatgcc aatgccgttg gccaccgtaa acagcccctg
      121 ctgctgacgg tctcgattga agcgcagcag tgcgtcgcgc atgtcgagag cagcgcgagc
      181 cgctctccac caatgcggag gcattccctg gcgttcacgg aatacggcca tgatcgcgtc
      241 acctacgtat ttgtcgatcg tcccgccctg ctccacgata caggcttcca tgaccgtaaa
      301 atagtcattg agcatggtaa cgatgacttc aggctcgaaa cgttccgaca agctggtaaa
      361 accgcgaatg tccgagaaga gaatcgtgac ttcggcccgt tccgcgtgct ccgtggcatt
      421 gtcctgccgc acggcctcga gcacgtcccc cgacacaaac cggctcatct gctcgcgttc
      481 gtacaagccc cgtgtcatgc gattgaaggc ttcaccaagt tctccgaact cgtcttttga
      541 gtcaagggcg acgcgaaccg agaaatcgcg gcccgagacg gcatccgcgg cggctcgcaa
      601 ttcgagcaga ggttgagcga acctgccgct cagaaagcgg gtgaccccga caagcaccag
      661 cagggcatac gcgaccagca gatagagacc ccagacgatc acatgatgcg accgcaggag
      721 aggacgcccg tcgagcccgg cgacgacaac atactgattg ctcgagatgc gccgcgcacc
      781 gatgacccag gggtccgagg aatcgaaata gtctcgcacc acggcttccc ggacgtctgc
      841 cgccagagca tgagcatcca gttcgcgcgc ggacagactc gtgcctgcgg gtcggacttt
      901 caaggtgttg ccttcgcgac gcaccacctt aagggtattc actcccatgg tccgcagtgg
      961 atcggtttcc cggaacagct cctgatcgat gaaacgtggc aggctgttat acacgctctg
     1021 catcagcacg atgactccgg ctacctctcg acgctcgaga tctcgataca gaatatcgat
     1081 gaaaaaggtc atcggttcac ggtctccagt cgccaggtaa aaaaaacgat cgagagacac
     1141 catgaactca gccagcgact gggccatcag cagtttgaag agatcatccc ccatggccgc
     1201 ttccgccagg tccgccacgc ttttggcacc gggggtctcg agtctcaggg ccttgaacaa
     1261 cttgaaggcc acccccttga agatcgattg cagaagtcga tcttccttgg tgtcattgcg
     1321 aatcagcttg ccctcggtgg tgataatgaa gaaaccttga atttcacgct gggtggatag
     1381 gacatcgagc ctctgtcgca cggtctccgg gtcggaaaaa tgctgaccgg cccaatcgcg
     1441 cagttcccgg aactttccga gttgccgttc cataaaactg cggtatcgct gatcgatcgc
     1501 ggccagtcgc cactcgagtc gctttttgag ttgttcgcgc tcaaaggagt aatgaaacga
     1561 cgcatacgcc caggcgacca acgtgaagcc gatcaatggc agcagtgcgc tgattgtcaa
     1621 agcaaggttg agctgaccgt agattcgtag cgcaaccggt ttttcctgaa ggtgccagcg
     1681 ccagagtaca agtccactga agagaaagaa tagaagcagg gccagatcca cccacggaag
     1741 atagcgtcgt gcgggaacgg ccaacagacg aaccggaaca taggccagca gatggatttc
     1801 cggatggtga cgggccacat cgacccagcc aaggccccca acggtcggca accaggtacg
     1861 ttcgggacga gtcggcgacg atggcgccca ccgccgcagg accgcggcat cacgtctttc
     1921 gcgacgcgcg cgctcaaaga cccacgaggg cgcgatccgg gtctcgggga tggtcaggag
     1981 cactccaccg cgcggtcgcc cggtcacctt gtcgcgcaga atcgccgcca ccgtcgactt
     2041 gcggtccatg accgtacggc cggccactgt cccaaacagc ggcgacacat ccccgatcaa
     2101 ccccgccagc gtcgccacga catccgcctc aatatccgcc cacgcgctca tgcca
//
LOCUS       DAORHD010000912         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63752_length_2154_cov_3.698428, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000912 DAORHD010000000
VERSION     DAORHD010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63752_length_2154_cov_3.698428"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PKO06_16950"
     CDS             <1..647
                     /locus_tag="PKO06_16950"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71394.1"
                     /translation="RLFYGAQLAAVIGQETFLKGLIERIQKVPLNDELAQLSQATSRL
                     LEDRGYRHLAIKVMESALAQRPDDMDLQFALSEEYFLENRSAEAVKLLEDVVSKDASR
                     VRAYLLLIDSLAACGRVDEAHQRAIDRMDDLSIPDLVRGQIRSRAILLADKISPERKA
                     SFGGSTLAPTPADASPTAASDPTPAWPTPMPAAATESGAVATTTESTDQQSANK"
     gene            657..800
                     /locus_tag="PKO06_16955"
     CDS             657..800
                     /locus_tag="PKO06_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71395.1"
                     /translation="MLIEIFTDGKVVVDGLEVGKGRAETILRDYLLNPQSLMARKQDT
                     KAA"
     gene            complement(868..1722)
                     /locus_tag="PKO06_16960"
     CDS             complement(868..1722)
                     /locus_tag="PKO06_16960"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71396.1"
                     /translation="MFEEELSKLKSKAEADPRNPEPYKEIGNLLFHQEKYEEAIEAYQ
                     KALDLEPKYYKALYNLGNTYYKLEQFEKAVHYWHMAIEAKPDFDHAYFNLGYHYFQKG
                     FLREAIRSLGEAARINPTAADTHNYLGQAFAQANQLPQAIDEFKKAMALAPDDPDIRY
                     NLANTSYDNGDFETAAREWRETLERRPKDAKARNNYCDALLQLNRLDEALEQIEIVLN
                     QEPEYPPALCTKAEILDKVNRREEAVELFRKVATLTEGNTSWELLYNYVRQRLMDVPE
                     MNELPKKH"
     gene            1841..>2154
                     /locus_tag="PKO06_16965"
     CDS             1841..>2154
                     /locus_tag="PKO06_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV71397.1"
                     /translation="MLSQMAASALELELWEYPRWLEEGEKVDRFAWVKKRLKQFEAEN
                     PGITVKLTELTWARGDEKLKIAALGGCAPDLAPGTVPLLYIREELVVPVDEYMTEAEK
                     ADL"
BASE COUNT          427 a          584 c          657 g          486 t
ORIGIN      
        1 gacggttgtt ctatggggcg caattggccg cggtgatcgg ccaggagacc ttcctgaagg
       61 gcttgatcga acgcattcag aaggtgccgc tcaatgatga actggcgcaa ctctctcagg
      121 ctacgagccg cctgctcgaa gatcgtgggt atcgacacct ggcgatcaaa gtcatggaaa
      181 gcgctctggc acagcgtcca gacgacatgg atctgcaatt cgccttgtct gaggagtatt
      241 ttctcgagaa tcggtccgct gaagccgtga agctgctcga agacgtggtc agcaaggacg
      301 ccagccgggt tcgggcgtat ctgctgctga tcgactcact ggcagcctgc ggcagagttg
      361 acgaagccca tcaacgggcc attgatcgca tggacgatct gtccattccc gatctggtgc
      421 gcggccagat ccggtcacgc gccattctgc tggcggacaa gatcagcccg gagcggaaag
      481 ccagcttcgg cggatcaacc ctcgcgccga ctcccgcgga tgcgagcccc acggcggcat
      541 cagacccgac gccggcctgg ccgacgccga tgcctgccgc cgcgacggaa tcgggagcag
      601 tcgctacaac aacggaatca acggatcaac aatccgcaaa caaataagga ggtttgatgc
      661 tcatcgaaat ctttacggac gggaaagtgg tcgtagacgg tcttgaagtc ggcaagggac
      721 gggcggaaac gattctgcgc gactatctgc tgaacccgca atcgctgatg gcgcgcaaac
      781 aggataccaa agccgcctga catgcgctgg aacaacaacg agcgccgggg tctgcccccg
      841 gcgctcgtgt tgttctaggc gatcgactca gtgctttttg ggcagttcgt tcatctccgg
      901 aacgtccatg aggcgctggc gaacgtagtt gtacaggagt tcccaggaag tgttgccctc
      961 ggtgagggtc gcgaccttgc gaaacagttc gaccgcttct tcccggcggt tgaccttgtc
     1021 gagaatctct gccttggtgc agagcgctgg cggatactcg ggttcctggt tgagaacgat
     1081 ttcgatctgc tcgagggctt catcgagacg attgagctgc agcagggcat cgcaatagtt
     1141 gttgcgagcc ttggcatctt tggggcggcg ttcgagcgtt tcgcgccatt cgcgggccgc
     1201 ggtctcgaag tctccgttgt cgtaactggt gttggcgagg ttgtagcgga tatcgggatc
     1261 atcgggggca agcgccatgg ccttcttgaa ctcgtcaatg gcttgcggca gctggttggc
     1321 ctgcgcgaat gcctgaccca gatagttgtg ggtatcggcg gctgtcggat tgatgcgcgc
     1381 tgcttcgccg agtgagcgaa tcgcttcccg caaaaatccc ttttgaaaat agtgataccc
     1441 gaggttaaaa tacgcgtggt cgaaatcggg cttggcttca atggccatat gccagtaatg
     1501 cacggccttt tcgaactgtt cgagcttgta atacgtattc ccgaggttgt acagtgcctt
     1561 gtagtacttc ggttcgagat cgagggcctt ctgataggct tcaatggcct cttcgtactt
     1621 ttcctggtgg aacaggaggt tcccgatctc cttgtacggt tcagggttcc gcgggtcggc
     1681 ctcggctttt gattttaatt tagagagttc ttcttcaaac atggtcttcc ccaaacgaga
     1741 ttttcctgac aatcgcattg taatgcaaag tttctacccc tgcaagcccc atacaaagag
     1801 ctttttccgg gctttggtcc tgctggtgtt cctcttgacc gtgctcagcc agatggcggc
     1861 ttcggcgctc gaactcgagt tgtgggaata tccgcgctgg cttgaggagg gcgagaaggt
     1921 cgatcgcttc gcctgggtca agaaacgcct gaagcagttc gaagccgaga accccggcat
     1981 caccgtgaaa ctgactgagc tgacgtgggc tcgcggtgat gagaagctca agatcgccgc
     2041 cctgggcggt tgcgctcccg atctggctcc gggcaccgta ccgctgctct atattcgcga
     2101 ggaactggtg gttcccgtcg acgagtacat gaccgaggct gagaaggcag atct
//
LOCUS       DAORHD010000913         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63819_length_2153_cov_2.742612, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000913 DAORHD010000000
VERSION     DAORHD010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63819_length_2153_cov_2.742612"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>2153
                     /locus_tag="PKO06_16970"
     CDS             29..>2153
                     /locus_tag="PKO06_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71398.1"
                     /translation="MALAFFALPLSFIGFELIALRHAVADEIDVREKEVLLSSLRALK
                     DHHQLLPLCADWLTASFRTIVRTPEPRRHIAALQQQVQKVFGLTMEFIVFTPSGVIDT
                     DLSTIQIPATVSLYLGSDLARLDSTDPGRENRFNKRLPEYQEFFGPWAYRLAEDRHLV
                     GLHQVRADNRRNTLYVSPQTASGRLLALIDIPQNLETYQLNWVLRQRLSAQPRERGAL
                     LSADWTRSDLERQLGEGGETGWQASLLMGSTCRQAMRVGDWLWAQYPIDQHSRLFLGR
                     PDDSRAQLEQYRDRLILVLCALVLGAAVVFVRLQSATGRWAPRLSIRLFLLLSYTVGL
                     PLLVFGLSARTLQRDLDEVYQRLVHSRHVAIIRELDRRYLDYLGYLEETLRSRFRLTS
                     DGPGAEEELKQRMRQIGSDLRPVSAVYIDPTGTARSEYGHENFSLPPALKKLVFTACA
                     SVIGTQEKATDRVADELTATVIDEYGVNVDMVVSIVTEHPETLIDFGFFSTNIALLLN
                     PVFLNNGATPFAALTAWDTDIVRWQFLQSHLERLQANLADTEIAVQNLTQAGGHLPAD
                     SVLQKAPSDILQSFTETRRPVTVPVTLASQTWLLTGTRGSQLKNVLCIAGSSPERRRG
                     EAGQFHRRVLTIGGLLALFGLLIARILSITFLGPIRALAEGVDALAAVGVDLTVAEGE
                     VQDAAGSVGREVHAVRGALRNRARGT"
BASE COUNT          394 a          628 c          618 g          513 t
ORIGIN      
        1 acgttcaagt ctctggctca gacttttcat ggccctggcg tttttcgctc tgccgctgtc
       61 gtttatcgga ttcgaactga tcgcccttcg tcatgctgtc gccgacgaga tcgatgtgcg
      121 tgagaaagag gtgctgttga gctcgctgcg agccctgaaa gaccatcatc aactcctgcc
      181 gctgtgcgcc gactggctga ccgcgtcatt tcggactatc gttcgcacgc cagagcctcg
      241 gcgacacatt gcggcgttac agcaacaggt acaaaaggtc tttggtctca caatggaatt
      301 catcgttttt accccaagtg gagtcattga taccgatctg tcgaccattc agattccggc
      361 gaccgtgtct ctgtatctgg gctctgatct tgccagactc gactccacgg atcccgggcg
      421 ggagaatcgt ttcaataaac gacttccgga gtatcaggag tttttcggtc cctgggctta
      481 tcgtctcgcc gaagatcgtc atctggtcgg gttgcatcag gttcgggccg acaaccgaag
      541 aaacacgctg tatgtttcgc ctcagacggc ttcaggaagg ttgctggcgc tgattgacat
      601 tcctcagaac cttgaaacgt atcaattgaa ctgggttctg cggcaaaggt tatccgcaca
      661 accacgtgag cgaggagcct tgttgtccgc cgattggaca cgcagcgatc tggaacgaca
      721 acttggcgag ggtggagaaa cggggtggca ggccagcctg ctgatgggtt ccacctgccg
      781 gcaggcgatg cgtgtcggtg actggctctg ggcgcagtat ccgatcgacc agcattcgcg
      841 ccttttttta ggaagaccag atgactctcg cgcgcaactc gaacagtatc gcgacaggtt
      901 gattctcgtt ctctgcgcac tcgtcctcgg ggccgcggtc gtctttgtcc gtcttcagtc
      961 tgccaccgga cgttgggctc cgcgcctgtc gattcggctt ttcttgctgc tgtcctacac
     1021 ggttggtctg ccgttgcttg tctttggtct ctcggctcga acattgcagc gtgatctcga
     1081 tgaggtatat cagcgactgg tacattctcg tcacgttgcc atcattcgcg agctcgatcg
     1141 gcgctatctc gattatctgg gatatctcga ggagaccctg agatcccggt tccgccttac
     1201 ttctgatggc ccgggggctg aagaggaact gaagcagcgg atgcggcaga ttggcagtga
     1261 cctgcggccg gtatcggcgg tgtacatcga tccaaccggc accgcccgat ctgagtatgg
     1321 tcacgaaaac ttttctcttc ccccggcact aaagaaacta gtctttacgg cctgtgccag
     1381 tgtcatcggc actcaggaga aagccacgga ccgagtggcg gacgagctta ccgcgaccgt
     1441 catcgacgaa tacggcgtga acgtggacat ggtggtttcg atcgtgaccg agcacccgga
     1501 aacgctcatc gatttcggtt tcttcagcac caatattgcc ctgcttctga atccggtgtt
     1561 tctcaacaac ggggcaacgc cgttcgcggc tctcacggcc tgggacaccg acatcgtgcg
     1621 gtggcagttt ttgcagtcgc atctggagcg tctgcaggca aaccttgcgg atacagagat
     1681 tgcggtacag aacctgactc aggcgggtgg ccatctccca gcagattcgg tgctccagaa
     1741 ggcaccatcc gacattctgc agagcttcac cgaaacccgc cgacccgtaa ccgtgccggt
     1801 cactctggct tcgcagacct ggctgctgac cggaacccgt ggttcccaac taaagaacgt
     1861 tctttgcatc gccggatctt cacccgaacg ccgtcggggc gaggccggcc agtttcatcg
     1921 tcgcgtgctg acgatcggcg gcttgctggc gctgttcggt ctgctcatcg cccgcattct
     1981 ctcgatcaca tttcttggcc cgattcgcgc attggccgag ggcgtcgatg ccctggccgc
     2041 cgttggtgtt gatctgaccg tcgctgaagg cgaagttcag gatgctgccg gctccgtcgg
     2101 ccgtgaggtt cacgccgtcc gaggtgctct gcgaaatcga gctcgtggca ccg
//
LOCUS       DAORHD010000914         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_63829_length_2153_cov_2.392278, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000914 DAORHD010000000
VERSION     DAORHD010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63829_length_2153_cov_2.392278"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..2060)
                     /locus_tag="PKO06_16975"
     CDS             complement(225..2060)
                     /locus_tag="PKO06_16975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV71399.1"
                     /translation="MWNQLVERCKDTFLKLRWKLFFVFGTLATLGVAVGGLLALFQTT
                     ERLNAEMQANLAGTAQLLSRSLDPARLDAVRSNSDPYYGEVKQFLQAAQEELNLSWIG
                     IYRYNGRYFTAVVDGEQAGNEFCCGFPIFNMPAELRQAWKGEPTPVSFSDDAYGIWLT
                     AYAPVRAENGEVIAVIDVSRDAEVLNRVREQTKFDTLKNIGLIILVTLTICMLFASGL
                     TRQLGRLTEGARMVSYGDLTVTIPVSSKHDEIGELVQTFNTMTDQLRRNKEDLERKIF
                     ELTLLFDISQKINYGSSTDEVLRMILDRFVQAMKAERGSILMFDEDQNHLTVSVAVGR
                     NVERMVKRVTLAPGEGVAGRVFQTMQPVIMNHVSEEDFKPFDDPIEFDVRNIMCLPLV
                     LERRPIGVINLVNKSLGEFSTADHTLGITLASQVALTIEKSRLWELAVTDGLTKLFVH
                     RYFQVNLEAEMKRALRYKKQLSMILMDIDHFKKFNDTWGHQTGDKVLWHVAQILKASL
                     RGPDIPARYGGEEMAVILPETDAAGAMLVAERIRKSVEEFEFPGPDHPLKVTLSLGVA
                     SCPEQATDRLDLIKKADEALYSCKHAGRNCARLYQPDLPSSPKHA"
BASE COUNT          448 a          622 c          612 g          471 t
ORIGIN      
        1 ctgaaccgtc agaatccctt cgatggtata tcgaaccgcc cacggctcgg cctgcaacgt
       61 gccgccaagc atcagaaccg tcaaccagag caccagtagc attcgtttca tcgtcttcat
      121 tcctccgttt tctcgttctc agagcggtaa tttgactatt ttacccgata acctgccatt
      181 tcggaagctc gattttcgcg ccgccggttg tccttggcag tgaatcaggc gtgtttgggc
      241 gaggatggaa gatcaggctg gtacagacgg gcgcagttgc gaccggcgtg tttgcaggaa
      301 tagagcgctt catcggcctt tttgatcagg tcgaggcggt ccgtggcctg ttctgggcac
      361 gaggcaacgc cgagactgag cgtcaccttg agcggatgat cgggaccggg gaactcgaac
      421 tcctcgactg acttgcggat acgctcggcg acaagcatgg cgccggccgc gtcggtttca
      481 ggcagaatca ccgccatctc ttcgccgccg taacgcgcgg gaatatccgg cccgcgcagg
      541 ctggccttga gaatctgcgc cacgtgccag agcaccttat cgccggtctg atggccccac
      601 gtatcattga acttcttgaa atgatcgata tccatgagga tcatcgacaa ctgcttcttg
      661 tagcgcaacg cgcgcttcat ctcggcctcg agattgacct ggaagtagcg atgaacaaac
      721 agtttggtga gaccgtccgt gaccgcgagt tcccagaggc gggatttttc gatggtcagc
      781 gcgacctgcg aggcgagcgt gattcccagc gtatgatcag cggtcgaaaa ctcgccgagg
      841 ctcttgttga ccaggttgat gacgccgatc gggcggcgct cgaggaccag cggcaggcac
      901 atgatgtttc gcacatcgaa ctcgatcggg tcgtcgaagg gcttgaaatc ctcttcggaa
      961 acatgattca tgatcactgg ctgcatcgtc tggaagacgc gccccgccac accctcaccc
     1021 ggtgcgaggg tcacccgttt caccatgcgc tcgacgtttc gacccacggc gaccgagacg
     1081 gtcaggtggt tctggtcttc gtcgaacatc agaatactgc cgcgttcggc cttcatagcc
     1141 tgaacgaacc ggtcgaggat catgcgcagc acttcgtcgg tgctgctgcc gtaattgatc
     1201 ttctggctga tatcgaacag cagagtcagc tcgaagatct tgcgttcgag gtcttctttg
     1261 ttgcggcgca actgatcggt catggtgttg aacgtctgta ccagttcgcc aatctcatca
     1321 tgcttgctgg aaaccggaat tgtgaccgtc agatcgccgt atgaaaccat gcgggcaccc
     1381 tcggtcaacc gtccgagttg ccgggtcagc cccgaggcga agagcatgca gatggtgagc
     1441 gtcaccagaa tgatcaatcc gatgttcttg agggtatcga acttggtctg ctcgcgtacg
     1501 cgattgagca cctcggcatc gcgactcaca tcgatcaccg caatgacttc accattctca
     1561 gctcgtactg gtgcataggc cgtcagccag attccgtagg cgtcatcgga aaacgaaacc
     1621 ggtgtcggtt cgcctttcca ggcctgtcgg agttccgcgg gcatgttgaa aatcggaaag
     1681 ccgcaacaaa actcgtttcc ggcctgctcg ccgtcgacga cggccgtgaa gtatcgacca
     1741 ttgtaccggt agataccgat ccaggagagg ttcagttctt cctgtgccgc ctgtagaaac
     1801 tgcttcacct caccgtagta gggatccgag ttcgaacgca ccgcatccag acgcgcgggg
     1861 tccagggatc ggctgagcag ttgggccgtg ccggccagat tggcctgcat ctcggcattc
     1921 agtcgttcgg tggtctgaaa cagggccagc agcccaccga ccgcgactcc cagggtcgcg
     1981 agcgtgccga acacgaaaaa cagcttccag cgcagtttga gaaacgtatc cttgcacctt
     2041 tcaacgagtt ggttccacat cgattatacc atttcagcga aaattcaaac cggacgaagg
     2101 tcgcgaacgg aacagtcgag tttcgttcgc atcacgacag ggtcgtctgc aga
//
LOCUS       DAORHD010000915         2149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64030_length_2149_cov_2.909265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000915 DAORHD010000000
VERSION     DAORHD010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64030_length_2149_cov_2.909265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /gene="galT"
                     /locus_tag="PKO06_16980"
     CDS             complement(<1..985)
                     /gene="galT"
                     /locus_tag="PKO06_16980"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV71400.1"
                     /translation="MPQLRKDPVLKQWVIISPERGKRPIHYKNENGLPEDNPKKCPFC
                     EGHESMTPEEVMAFRRNGTAANQEGWWVRVIPDREPILVPAGDSGKEGIGMFDAMNSI
                     GIHEVVVEVPRHDQNLQNVPLEQVREVIWAFKQRVLEIKKNPRFKHFMIVKNSGRGVS
                     SFSHSHSHIVATPIIPKRIEEEIDGAREYFHYHDRCIFCDIVRQEREDNTRIVFEDPQ
                     FLAFCPFASRFPFELFIIPKQHQPFFETLDNDQVHGFANALQICLKKLQSLLPDLPYN
                     FILHTSPCSDSYREFYHWHLEIIPKLTQVAGFEWGSGFYINPTPPEDAAQML"
     gene            complement(1017..2015)
                     /gene="galT"
                     /locus_tag="PKO06_16985"
     CDS             complement(1017..2015)
                     /gene="galT"
                     /locus_tag="PKO06_16985"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV71401.1"
                     /translation="MPQLRKDPVTKRWVIIVHEQARKPGDFTWKKDNKQSGKCPFCPG
                     AEANTPPEVMSYREPNTLPNQPGWKVRVIPNKFPALQIEGDLDRTGIGIYDMMNGVGA
                     HEVIIETPEHDQGFDAYSYEQVLKIINAYADRYRDLRRDRRFRYILLFKNKGGSAGAS
                     LDHPHSQLIATPIIPKRVMEEVEGARQYYYYKERCVFCDVIRQELSGGQRIIMENEFF
                     VAFAPFASRFPFETWIAPKDHKASFGDIEDHEMEGFAEILHRTLTRLIKVLGDPPFNY
                     ILHTSPCDEHCAEYFHWHLEIMPRLSKVAGFEWGSGFYVNHVPPESAAKYLKQPGG"
BASE COUNT          443 a          604 c          632 g          470 t
ORIGIN      
        1 gcagcatctg agcggcatct tcggggggag tcggattgat gtaaaaaccc gaaccccact
       61 cgaacccggc cacctgcgtc agtttcggga taatctcgag gtgccagtga taaaactcgc
      121 ggtacgaatc ggaacagggc gaggtatgca gaatgaagtt gtagggaaga tcgggcagca
      181 ggctctgcag cttcttcagg cagatctgga gcgcgttagc aaacccatgc acctgatcat
      241 tgtcgagcgt ttcgaagaac ggctgatgct gtttcggaat gatgaacagc tcaaaaggaa
      301 agcgcgacgc aaagggacag aatgccagaa actgcgggtc ctcgaacacg atgcgcgtgt
      361 tgtcttcgcg ctcctggcga acgatgtcgc agaaaataca gcgatcgtga taatggaaat
      421 actcacgcgc gccgtcgatc tcttcctcga tacgcttggg aatgatcggc gtggcgacga
      481 tgtgtgaatg cgagtgcgaa aagctcgaca cgccgcggcc gctgtttttc acgatcatga
      541 agtgcttgaa tcgcgggttc ttcttgatct cgagcacccg ctgcttgaac gcccagatga
      601 cttcgcgcac ctgttcgagc ggcacattct gcaggttctg atcatggcgc ggcacctcga
      661 ccacgacttc gtgaataccg atcgagttca tcgcgtcgaa catgccgatg ccttccttgc
      721 cgctgtcgcc ggccggcacg agaatcggct cgcgatccgg aatgacgcgc acccaccagc
      781 cttcctggtt cgcggcggtg ccattacggc gaaacgccat cacctcttct ggagtcatgg
      841 attcatggcc ctcacagaag gggcactttt tcggattgtc ctcgggcagg ccgttttcat
      901 ttttatagtg aatcggtcgc ttgccccgct cgggtgaaat gatcacccac tgtttgagaa
      961 ccggatcctt gcgtaactgc ggcatgatgt cgcttccttc ggtgtcagcc ggccgatcag
     1021 ccgccgggct gcttcagata tttcgccgcg ctctcgggcg gaacgtgatt gacgtagaac
     1081 cccgaccccc actcgaaccc cgcaaccttc gacaggcgcg gcataatctc aagatgccag
     1141 tggaaatact cggcgcagtg ctcatcgcag ggcgacgtgt gcagaatgta gttgaatggc
     1201 gggtctccga gcaccttgat cagacgcgtc agcgtgcgat gcagaatctc ggcgaagcct
     1261 tccatttcat ggtcttcgat atcgccgaac gacgccttgt gatctttggg cgcgatccag
     1321 gtttcgaacg ggaaccgcga ggcaaagggc gcgaaggcaa cgaaaaactc gttttccatg
     1381 atgatgcgct ggccgccgct gagctcctgg cgaatgacgt cgcagaatac gcagcgctct
     1441 ttgtagtagt agtactgccg cgccccttcc acttcctcca tgacgcgctt gggaatgatg
     1501 ggcgtggcga tcagctgtga atgcggatga tcaagactgg ccccggccga gccgcccttg
     1561 ttcttgaaca gcagaatgta gcgaaaccgc cgatcgcggc gcaggtcacg gtaccggtcg
     1621 gcatacgcat tgatgatctt cagaacctgc tcgtaggaat acgcatcaaa gccctgatcg
     1681 tgttcgggcg tctcgatgat gacctcgtgc gctccgacac cgttcatcat gtcatagatg
     1741 ccgatgccgg tgcggtcgag atcgccctca atctgcagcg cagggaactt gttggggatg
     1801 acgcgcacct tccagccggg ctggttggga agcgtgttcg gctcgcgata cgacatgact
     1861 tcagggggag tgttcgcttc agcccccgga cagaacgggc attttccgga ctgtttgttg
     1921 tcctttttcc aggtgaagtc gccgggcttg cgtgcctgtt cgtgtacaat gatgacccag
     1981 cgcttggtca cgggatcttt gcgtaattgc ggcatgggga ctcctggagt cgaggacttg
     2041 aacgcgaggg aattatactc cttcgcaccc cgttgcgcaa cgctgcaaaa tcacgattcg
     2101 cagcttggaa gcgacgtgca ttggctcacg acccggggca cgtcattca
//
LOCUS       DAORHD010000916         2148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64066_length_2148_cov_3.451983, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000916 DAORHD010000000
VERSION     DAORHD010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64066_length_2148_cov_3.451983"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKO06_16990"
     CDS             complement(<1..604)
                     /locus_tag="PKO06_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71402.1"
                     /translation="MNTPHALCLATASFRLGRRNQRSNMLFLVVSLVLIILSQSVHAA
                     EQPGSDDDLHDAEAKALASLRLHVPADALSERLLTVLPRALFSGGAPEDDPDLSQYLV
                     FGQGGQLYVLGRRQKTIWSIHPTTGKIEVVYRDVRPGLLDFTDFTLLQNELLALADQP
                     RQAVYLFQQRRFWKGLGLLNDRWLFPSPRAVCTNDDRSLLG"
     gene            complement(601..1899)
                     /locus_tag="PKO06_16995"
     CDS             complement(601..1899)
                     /locus_tag="PKO06_16995"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV71403.1"
                     /translation="MSEPRRKRRCGQSFALWLLVLLCLPISITFGATEVDTTDDEEAV
                     IFGGRPPRSTPSSPPPTTTSGSTRQTPMPVSSTATSVAGTPDLIYAADDLLLGDPAAT
                     PTLSTADAEQALKRLLGKNAWLAPLLDLEKKNQRDLLEKILLLENTVKGIDWKSLRGF
                     RRSLQQQEMTQLWRLMQAFGIRKLSPGDFRNLQKIAGTIDTLKQLATRAGEREQLDRL
                     SYALAQTQELLSRYAPSPEQARQFKQQALQNYRGILKNRSGTTTEKPGQSVETSDVQT
                     RIFSLEQNFGGIQPLVAPSGKRVVLTSDLGMRVHPRTKKRKMHQGIDFAQVGCTGWPV
                     QAIGPGRVVKSGWENGYGYVVIVLHDIDGERLYTRSAHLKQAGRAAVGTVVPKGGILG
                     YCNSSGLVTGPHLHFEVRAQSEFGSVIDPKPIIAKGRTQP"
BASE COUNT          438 a          567 c          670 g          473 t
ORIGIN      
        1 ctcctagaag tgaacgatca tcgttggtgc agacggcgcg gggactggga aacagccagc
       61 ggtcgttgag cagccccagt cctttccaga atcggcgctg ttgaaacaga tatacggctt
      121 gccgcggttg atcagcgaga gcgagaagtt cgttctgcag aagggtgaaa tcggtgaaat
      181 cgagcaggcc ggggcggaca tcacgataga cgacttcgat ctttccggtt gttggatgaa
      241 ttgaccagat ggttttttga cgcctgccca acacgtacag ttgcccaccc tggccaaaga
      301 ccagatactg tgagagatcc gggtcgtctt ccggtgctcc tccactgaac aatgctcgcg
      361 ggagcacggt cagcagtcgc tcagaaagag catcggctgg gacatgcaaa cgaagactgg
      421 cgagtgcttt ggcttcagcg tcatgcagat catcgtcgct tccgggttgt tccgctgcat
      481 gaaccgactg gctcagaatg atcagcacga gcgagacaac cagaaacagc atgttcgaac
      541 gttgattccg tcgcccgagt cggaaagacg cggtggcgag acacagggca tgcggtgtgt
      601 tcacggctgg gtccttcctt tcgcgatgat cggcttggga tcgataactg acccgaactc
      661 tgattgagcg cgaacttcga aatgcagatg cgggccggtt accaaccctg agctgttgca
      721 atagccgagg atgcctccct tgggcaccac ggtgccgacg gcggctcttc cggcttgttt
      781 caggtgcgcc gaacgtgtgt agagtcgctc gccatcaatg tcgtgaagaa cgatgacgac
      841 atacccgtag ccgttttccc agccgctttt cacgacgcga ccggggccga tggcctgcac
      901 cggccagccg gtacagccga cttgggcgaa gtcgatgccc tgatgcatct tccgcttttt
      961 cgttctgggg tgaacgcgca tgccgaggtc agaggtgagg acgacgcgtt tgccagaagg
     1021 agctacgagc ggttggatgc cgccgaagtt ctgctccagg ctgaagatac gggtttgcac
     1081 atctgacgtt tcgaccgact gccccggttt ttctgtcgtg gttcccgagc gattcttcag
     1141 aataccccgg tagttttgca gtgcctgttg tttgaactga cgggcttgtt ccggcgacgg
     1201 ggcgtagcga ctcagaagtt cctgggtttg agccagtgca taagaaagac gatcgagttg
     1261 ttcacgctcc ccggcacggg ttgccagctg cttcagggtg tcgatggtgc cggcgatctt
     1321 ctgcaggttc ctgaagtcac cgggagaaag cttgcgtatg ccgaacgcct gcatcagacg
     1381 ccagagctgg gtcatctcct gctgctgcag tgaacgtcga aagccgcgaa gcgacttcca
     1441 atcgatgcct ttcacggtgt tttcgagcaa cagaatcttt tcgagcaggt cgcgctggtt
     1501 cttcttttcg aggtcgagca aaggtgccag ccaggcgttc tttccgagca gacgtttcag
     1561 ggcctgttcg gcatctgccg tcgaaagggt tggcgttgcg gccgggtcac caagcagaag
     1621 atcatcagct gcatagatca ggtcaggtgt gcccgcgacc gaggtggcag tcgaggagac
     1681 gggcattggt gtctgccggg tgctgcctga ggtggttgtg gggggcggcg aagaaggtgt
     1741 ggacctgggc gggcgtccac caaagatcac ggcttcctcg tcatcagtgg tgtcgacttc
     1801 ggttgcgccg aacgtgatgg aaatgggcag gcacagcagc accaacaacc acagcgcgaa
     1861 gctttgtccg cagcgtctct tgcgtcgtgg ctcactcatg ggaccctcct gtctcttcat
     1921 ggtcattgta tcgcaatcgt ccctggcatg cacccgagag agcttgcagg aaattgtttc
     1981 ccccatgcat tgagtggtgc tagaatgccg gcgaaaagtt cgcacctgga gggaaggaat
     2041 gagtcttcgt atcgacgagc atccgattct gacctttaaa aagggtaagc gggtcacgtt
     2101 cttcttcgaa ggacagaaaa tggaagggtt cgaaggagag cccattgc
//
LOCUS       DAORHD010000917         2147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64121_length_2147_cov_3.271989, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000917 DAORHD010000000
VERSION     DAORHD010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64121_length_2147_cov_3.271989"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKO06_17000"
     CDS             <1..1049
                     /locus_tag="PKO06_17000"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV71404.1"
                     /translation="KKRKCMNSHLNRDGSLLRSCLIGVVAACLVALVCVSIGPKQAVS
                     VFDLTLWQQFWRGEALSTDARILFQIRFPRVLLGFLVGGSLSLAGLLFQSLLQNPLAD
                     PFIIGVSGGSALAAVLAQTLLGLHDPLLIVLIAFGGGLAATVFVHLVALRAGTLHRGV
                     IILTGVVMNAFFSAAISLTLLLAGRDMPRIFAWLMGSLNLPEPTLLLPMFTLALVVMA
                     VVFACAHPLNLLSLGDFQAYHLGLAPERMKWLVLAAASLLTSIAVSLSGMIGFIGMFV
                     PHAVRLTLGFDHRFLIPWSFLAGAMVLAGADTLVRLAPTGEVPVGTVMAFLGAPVFLH
                     ILFHRVSRVSWQES"
     gene            1051..1863
                     /locus_tag="PKO06_17005"
     CDS             1051..1863
                     /locus_tag="PKO06_17005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71405.1"
                     /translation="MTDAPGLAVKGLSFEYERRSVLHDVSLSLARGELVALLGINGCG
                     KTTLLRCLTGYLKRKNGEINWDGEPLDQLDVRRRAQTVFLSETNHVPPFPCTVGTLIM
                     LGRSAFADRWGNLSSDDRERALRAAAALHVDTMLARPYQELSQGERQRVRLAMALAAD
                     TPYLLLDEPTSHLDPSQQALVLRLLSEYVRESRHGVLAVLHDINLARLFDRILVLHQG
                     SIGAADCPGRVIEKKTLDRIFGADLFEIMGPHDAPIGVLRDPRPRGREGMKL"
     gene            complement(1907..>2147)
                     /locus_tag="PKO06_17010"
     CDS             complement(1907..>2147)
                     /locus_tag="PKO06_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71406.1"
                     /translation="PSGEATGWIADAGVTVPGLATVTTAGFAAGLKLLLAIAGARKGT
                     SWVAVAVFVDALFPCAKAADERCSAAQTPQHPEKA"
BASE COUNT          351 a          605 c          673 g          518 t
ORIGIN      
        1 gcaagaaacg gaaatgcatg aattcgcatt tgaatcgtga tggctcactg cttcgctcct
       61 gcctgatcgg ggtcgtggca gcctgcctgg ttgctctggt ctgtgtctcc atcggcccga
      121 aacaggcggt gagcgtcttt gacctgacct tgtggcagca gttctggcgt ggggaagcgc
      181 tctcgaccga tgcgcgcatt ctctttcaaa ttcgtttccc gcgagtgctg ctgggttttc
      241 tggttggcgg cagcctgtcg ctcgccggct tgctctttca gagtctgctg cagaacccac
      301 ttgccgatcc atttatcatt ggggtctctg gcggaagcgc tctcgcggcg gttcttgccc
      361 aaacactgct gggactgcac gatccgctgc tgattgtgtt gattgccttc gggggaggtc
      421 tggcggcgac ggtgttcgtg catctggtgg ctttgcgtgc gggaactctg catcgagggg
      481 tcatcattct gaccggcgtg gtcatgaatg ctttcttctc tgcggcgatc tccttgacgc
      541 tgttgctcgc cgggcgtgat atgccgcgta tcttcgcctg gctgatggga agcctcaatc
      601 tgcccgagcc gacgctgtta cttccgatgt tcactctggc cctggtggtg atggcggtcg
      661 tgtttgcctg cgcgcatcct ctcaaccttc tgtcgctcgg agattttcaa gcgtaccatc
      721 ttggtctggc accagagcgc atgaaatggc tggttctggc cgcggcttcg ctattgacct
      781 cgatcgcggt gtctctgtcg ggaatgatcg ggttcatcgg catgttcgtt ccgcatgcgg
      841 ttcgcctgac cctcgggttc gatcatcggt tcctgatccc ctggtctttt cttgccggag
      901 cgatggtgct ggcgggagcg gatactctgg tgcgactggc accgaccggg gaagtgccgg
      961 tgggtacggt tatggcgttt cttggcgcgc ctgtctttct gcatattctg tttcatcgcg
     1021 tgagtcgcgt gtcatggcag gagtcttgat atgaccgatg ctcccggttt ggcggtgaag
     1081 gggttgtcgt ttgagtatga acgacgcagc gtcctgcacg acgtgtcact gtcgctcgcc
     1141 cgcggagagc tggtcgcttt gctgggaatc aacggttgcg gcaaaacgac attgttgagg
     1201 tgcttgaccg gttatctgaa gcgaaaaaat ggcgagatca actgggacgg agaacccctg
     1261 gatcaactcg acgtgcgtcg ccgggctcaa acggtatttc tctcggaaac caaccatgtg
     1321 ccgccgtttc cctgcacggt tggcactttg atcatgcttg gtcgcagcgc gttcgccgac
     1381 cgatggggca atctttcgtc tgacgatcgc gagcgggctt tgagagcggc agcggcgctt
     1441 cacgtcgaca ccatgctcgc gcgaccctac caggaactca gccagggaga acggcagcgg
     1501 gtcaggctgg cgatggcgct ggcggcggat accccgtatc tgctgctgga cgagccgacc
     1561 tcgcatctcg atccgtcgca acaggcgctg gtattgcgcc tgctgtcaga gtatgtacgt
     1621 gaatcccgac acggggtgct tgccgtgctg cacgacatca acctggctcg actgtttgac
     1681 cgcattctcg ttttgcatca aggcagcatt ggcgctgccg attgtcccgg cagggtgatc
     1741 gagaagaaga cgctcgatcg catcttcggt gccgatctgt tcgagatcat ggggccgcac
     1801 gatgcgccga tcggagtgct gcgtgatccg cggcccaggg gaagggaagg aatgaaacta
     1861 tgaaacgaat accagcctgg tggcgagtcg ctgtatttgc tgcctgctac gctttctcag
     1921 gatgctgcgg tgtctgggcg gctgaacaac gctcgtctgc cgctttggca cacggaaaaa
     1981 gtgcatcaac aaagacagct actgcaaccc aacttgtacc ctttcgcgcc cccgcgatag
     2041 cgagcagcag cttcaaaccg gccgcgaaac ctgcggtggt gacggtcgcc aaaccgggga
     2101 ccgtgactcc ggcatcagcg atccagccgg ttgcctcgcc ggacggt
//
LOCUS       DAORHD010000918         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64260_length_2144_cov_4.790330, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000918 DAORHD010000000
VERSION     DAORHD010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64260_length_2144_cov_4.790330"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKO06_17015"
     CDS             complement(<1..1030)
                     /locus_tag="PKO06_17015"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNV71407.1"
                     /translation="MRTRGLGLFSKILLWFFGTVLIVAAITAVMIVYLQQSIESEHLQ
                     NASSFLQREAQTFIQAFEMDPAARLSPPATFPSRIWLFDASGTMLIQATFGPGFRPPL
                     PYRDGAPHPHSPSLPDCSAPVPPEDSRDTNRSSEQARFLSEIASAAVELLRSNEPQLI
                     FSVSDEDIVGKHLISANGNHYAALLHFKPRPFPFFFRWVTKPGAWLRLLLLTGVVTLL
                     CRALAQYLVWPVLELQNMSRSIAAGNFQKRLPESLTTRSDELGRLACDFNLLAEGIEA
                     SLKGQKQLLRDVSHELRSPLARVRVCLELLRSGQEEAREGLITKMERDVDRLNELIQQ
                     ILDLSRLDQ"
     gene            complement(1027..1776)
                     /locus_tag="PKO06_17020"
     CDS             complement(1027..1776)
                     /locus_tag="PKO06_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV71408.1"
                     /translation="MDKDRSPQRILILDDDIELCELLHTFLTGEGFIITVRHDPQEIL
                     NLSLEAEFDLVILDVMLPHINGLEVLKRIRTHSAVPVIMLTARGEEIDRIVGLELGAD
                     DYLPKPFNPRELSARIRAVIRRGAQKSNDSGTAPQPPHPESVQVGDVRLMLGSRQALC
                     NNEEVPLTGVEFRLLEVLMRSAGTVVKRQDLALQVLERHLSYEDRSLDVHISNLRRKL
                     GKHPSGGERIQTIRGIGYLFSVFENTSEEKL"
     gene            complement(1922..>2144)
                     /locus_tag="PKO06_17025"
     CDS             complement(1922..>2144)
                     /locus_tag="PKO06_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71409.1"
                     /translation="DLKINKSQETDPDADKLQTGTYAIVARGWTQINDFLSKIAPHAV
                     WYIPSDIGPTTMNQQNVWKGDLKIRWKVQ"
BASE COUNT          508 a          521 c          636 g          479 t
ORIGIN      
        1 gctggtccag gcgtgagagg tcgagtatct gctgtatcag ttcgttaagg cgatcaacgt
       61 cacgttccat tttcgtgatc aggccttcgc gagcctcctc ctggccggaa cgcagcagtt
      121 cgaggcagac tcgcacccgg gcgagaggag aacgaagttc gtgtgagaca tcacgcaaca
      181 gttgtttttg ccccttgagt gatgcttcga tgccttcggc aagcagattg aaatcgcagg
      241 ccagacgacc gagttcgtca gagcgggtcg tcaacgactc gggaagacgt ttctggaagt
      301 ttccggcggc gatgctgcga ctcatgttct gcagttcgag aaccggccag acgaggtatt
      361 gagccagcgc tcgacacaga agggtcacga ccccggtcag caacaacaag cgcagccagg
      421 cacccggctt ggtgacccag cgaaagaaga atggaaaagg tcgaggtttg aaatgcagta
      481 aggcagcata gtgattcccg tttgcactga tcagatgttt ccccacgatg tcttcgtcag
      541 agacactgaa aatcagttgc ggttcgttgg atcgcagaag ttcgaccgcc gcagaagcga
      601 tttctgacag gaaacgagcc tgttcagaag accggttggt atcacgggag tcttcgggtg
      661 gaaccggggc tgagcaatct ggaagagagg gggagtgagg atgaggagct ccatcgcgat
      721 aagggagagg cggccgaaag cccgggccaa aagtcgcctg gatcaacatg gttcctgatg
      781 cgtcaaacag ccagatgcgg ctggggaacg ttgctggcgg tgaaagtcgc gccgctgggt
      841 ccatttcaaa agcctgaatg aacgtttgag cttcacgctg cagaaaagaa ctggcgttct
      901 gcagatgctc gctttcgatc gactgctgca ggtagacaat catgaccgcc gtgatagcgg
      961 caacgatgag aaccgtgccg aagaaccaga gaagaatttt cgaaaagagt cccagcccgc
     1021 gagttctcaa agcttctcct ctgatgtgtt ttcaaatact gaaaacaggt atccgattcc
     1081 acgaatggtt tgaatgcgct ctccgcccga aggatgcttg ccgagtttgc ggcggagatt
     1141 actgatgtga acgtcgaggc tgcgatcttc atacgagaga tggcgttcga gcacctgaag
     1201 cgcgagatcc tgccgcttga cgaccgttcc cgcactgcgc atgagtactt ccaacaggcg
     1261 aaactcgact ccggttagag gcacttcttc gttgttgcac aaggcttgac ggctcccgag
     1321 catgagtcgg acatcaccaa cctgtactga ttcagggtgg ggcggttgag gtgccgttcc
     1381 ggagtcgttg ctcttctgtg cgccacgtct gatgacggct cgaatgcggg cggagagttc
     1441 tcgaggattg aagggtttgg gcagataatc gtctgcgccg agttcaagac cgacaatgcg
     1501 gtcaatttcc tcgccgcgtg ctgtcaacat gatcacggga acggccgagt gcgtgcgaat
     1561 gcgcttcagg acctccaaac cattgatgtg aggaagcatc acatcgagga tgaccaggtc
     1621 aaattctgct tccaggctga gattcaggat ttcctgagga tcgtgacgaa ctgtgatgat
     1681 gaatccctca ccggtaagga aagtatgtaa cagctcgcac agttcgatgt cgtcatcaag
     1741 aatcagaatg cgctgtggag atcgatcttt gtccatggaa aaattgtacc cgtattgatg
     1801 agatgacttc aatggaactg gctgttaacg aatgttaaga aatgcaaagc ccagtcatct
     1861 ttcgtgatac agagaaaaac ctccggccga tgtaccggcc ggaggtttca tgcggatcga
     1921 gttactgaac tttccagcga atcttcagat cgcctttcca gacattttgt tgattcatgg
     1981 tggtcggacc gatgtcgctg gggatatacc agacagcatg cggtgcgatc ttgctgagaa
     2041 agtcgttgat ctgggtccag ccacgagcga cgatggcata ggtaccagtc tggagcttgt
     2101 cggcatccgg gtcggtttcc tgcgatttgt tgatcttgag atcg
//
LOCUS       DAORHD010000919         2144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64264_length_2144_cov_3.862135, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000919 DAORHD010000000
VERSION     DAORHD010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64264_length_2144_cov_3.862135"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /gene="nusA"
                     /locus_tag="PKO06_17030"
     CDS             complement(<1..970)
                     /gene="nusA"
                     /locus_tag="PKO06_17030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNV71410.1"
                     /translation="MSSNNLYLALREISSEKNLPMQVLMNAIQEALVIAYRKNFDSEE
                     DVTVTLDEKTGLFRVVARRTVVENDDAVEEVDRQYTLEEAREINPDANVGDTIEEDVT
                     PASFGRIATQTAKQVVSQKIREAERNIIYQEFMDRVGQVLSGKVQRVERGTVYVEFAR
                     AEAVLPPKEQIPGEVFRTNDRIKCYVTEVKTTPRGPQIVLSRSAPKFVEKLFEQAVPE
                     IPERIVEIKSVSREPGLRVKIAVHSKEERVDPVGACVGLKGSRIKTVVDELNGEKIDI
                     VRWSQNLSDFITNALSPAKVLSVEINNELKSALVIVDPTQLSLAIGK"
     gene            complement(984..1475)
                     /gene="rimP"
                     /locus_tag="PKO06_17035"
     CDS             complement(984..1475)
                     /gene="rimP"
                     /locus_tag="PKO06_17035"
                     /inference="COORDINATES: protein motif:HMM:NF036577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNV71411.1"
                     /translation="MTDSSFQSIVVDRVRTFVESTFSGKVDVYDVLFRAGKRRCTLEV
                     KIDSPTGVTVSDCETVSRALSKHLDEVDLIHLPYNLEVSSPGAERRLLRPVDYERQVG
                     RLVRWVLKPQAEGPKEVFQARLQEFNPPTIRVSTEKELREFPLSQVDDARVVLEFPRK
                     KKD"
     gene            1664..>2144
                     /locus_tag="PKO06_17040"
     CDS             1664..>2144
                     /locus_tag="PKO06_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV71412.1"
                     /translation="MLSAIVKLMRSLALFCVVTGLLIFLGCLGAGGGGSLGRSPIDPA
                     DTNLVSGIAYFADRQLFGGIPILARDANTGAVGGLVRTDSRGAFAFTSLPPAIYNLTA
                     TTGNGETTFYSGLQVTGQQAQAIPEASLLGLKDLIIDRVSTASVRITFHSNLEAISQI
                     "
BASE COUNT          389 a          623 c          627 g          505 t
ORIGIN      
        1 ccttgccgat tgcgagcgag agctgcgtcg ggtcgacgat cacgagggct gacttgagtt
       61 cgttgttgat ctcgacgctg agcactttgg ccgggctcaa cgcgttggtg atgaaatcgg
      121 acagattctg gctccaccgc acgatgtcga tcttctcgcc gttcaactca tcgacgacgg
      181 tcttgatgcg gctgcctttc aaaccgacgc aggctccgac cggatcgacc cgctcttcct
      241 tgctgtgcac cgcaatcttg acgcgcagac cgggctcgcg cgatacgctc ttgatttcga
      301 cgatgcgttc cgggatctcg ggaacggcct gttcgaacag tttctcgaca aacttgggag
      361 ccgagcgtga aagcacgatc tggggtccgc gcggggtggt cttcacttcg gtgacgtagc
      421 acttgatacg gtcgttcgtg cggaacactt cgccgggaat ctgctctttc gggggcagaa
      481 cggcttcggc gcgggcgaac tcgacataga cggtgccgcg ttcgacgcgc tgcaccttgc
      541 ctgacagcac ctgcccgact cgatccatga attcctggta gatgatgttg cgctcggctt
      601 cgcgaatctt ctggctgacc acctgtttcg cggtctgggt cgcgatgcgg ccgaacgagg
      661 ccggggtcac gtcttcttcg attgtgtcgc cgacgttggc atcgggattg atctcgcgag
      721 cttcttcgag ggtatactgg cgatcgactt cctcaacggc gtcatcgttt tcgaccacgg
      781 ttcgccgggc gacaacccgg aacagccccg ttttttcatc gagggtgacc gtgacatctt
      841 cttcggagtc gaagttcttg cgataggcga tcaccagggc ttcctggatc gcgttcatca
      901 acacctgcat gggcaggttc ttttcgcttg aaatctctcg caatgccaga tacaggttat
      961 ttgaactcat ggcggcctcc cggtcaatcc ttctttttcc tggggaattc gagcacgact
     1021 cgggcgtcat cgacctgact cagcgggaac tcgcgcagct ccttttcggt gctgacgcga
     1081 atggtcggtg ggttgaattc ctgcaaacgg gcctggaaca cttctttcgg gccttccgcc
     1141 tggggtttga gaacccagcg aacgagccgc ccgacctgac gttcgtaatc gaccgggcgc
     1201 agcaggcgac gttcggcacc cggtgacgac acttcgagat tatacggcaa atggatcaga
     1261 tcgacttcat cgaggtgctt gctcagcgct cgcgagacgg tctcgcagtc cgacaccgtg
     1321 acccccgtcg gcgagtcaat cttgacttcg agcgtgcagc ggcgctttcc ggcgcggaac
     1381 aacacgtcat agacgtcgac ctttccggaa aaggtcgact cgacaaaggt gcgcacccgg
     1441 tcgacgacga tgctctgaaa gctgctgtcg gtcacgtctc catcctttcc gttttcgata
     1501 aaacagtcaa gccgcgcctt ccggggcacg gccgatgaga ggtcaaatta gtgcgcaagt
     1561 ctagcacgaa tgccccccga acgcaacttg tcgatcgacg ccggacggtt gttcaatttt
     1621 ccgtttggaa cggtatcgac aacctggggt cttggaaggt tcggtgcttt ctgctatagt
     1681 gaagctcatg cgatcattgg ctctgttctg tgtcgtgacc ggtctcctta ttttcctcgg
     1741 gtgtctcggg gcgggtgggg gcggtagtct tggtcgcagt ccgattgatc cggcggacac
     1801 caatctcgtc agtggcatcg cgtactttgc agatcgacag ctctttggcg gaatccccat
     1861 tctggcgcgt gatgccaata cgggtgccgt cggggggctg gtcagaacgg attcgcgagg
     1921 ggcttttgcg ttcacgtcgc ttcccccggc catctacaat ctgacggcaa ccaccggcaa
     1981 cggtgagacg accttttatt ccggtctgca ggtgaccggc cagcaggcgc aagcaatacc
     2041 agaagcatcg ctcctcggat tgaaagacct gattatcgac agggtttcta cagcttccgt
     2101 gcgaatcacc tttcactcaa acctggaggc aatctctcag attg
//
LOCUS       DAORHD010000920         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64449_length_2140_cov_3.971223, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000920 DAORHD010000000
VERSION     DAORHD010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64449_length_2140_cov_3.971223"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1628)
                     /locus_tag="PKO06_17045"
     CDS             complement(99..1628)
                     /locus_tag="PKO06_17045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNV71413.1"
                     /translation="MQTLKPLKVANALAAPASDLPEVRISELREWLRNRVNGGANLSA
                     LFACPVAEDLKRLRLVGVMSDPSRGSIELASAVVEEEYPALTPDCPAAHWFEREIAEQ
                     WGLRPLGHPWLKPIRFHRSYRPGFDAWDRSAEEPLLPCVTNFYRVEGDEVHEVAVGPV
                     HAGVIEPGHFRFQCHGENVFHLEISLGYQHRGIERALRGGPTPRTQLLMETLAGDTTI
                     GHGLAYVQLREALGAAKVPARAHVLRGIALELERLANHVGDLGALANDVGFLPTASYC
                     GRLRGDFLNLSAMICGSRFGRNLLTPGGVRFDLDAERLKKFSDRFHEVVIDTENAIQL
                     LWDSSSVLARFEQTGTVPLGMARDLGLVGVAGRASGIEHDVRKDFPRGIFQFHQIPVS
                     AWSTGDVFGRAYVRWLEIQRSEGFIKTMLRSLPATPIVGECQNPEPGRVVVSLTEGWR
                     GAVCHAAMTDAEGRFLWYKVVDPSFHNWMGLAMALRGQQISDFPLCNKSFNLSYCGHD
                     L"
     gene            complement(1632..>2140)
                     /locus_tag="PKO06_17050"
     CDS             complement(1632..>2140)
                     /locus_tag="PKO06_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV71414.1"
                     /translation="GVGGGGVFAAMYHAVNHSLSKGLLFLSAGRILDAFHTKAIAEVH
                     GIKHILPRTALFWIAGFLAITGTPPFGTFVSEFLVAQYILTSGRLLLGVVYVLLLAVI
                     FVGMVGPFIHMSYGTSPERLGKKAHAESILQMVPLTILLLGVLIMGIAPPTRVLAVMQ
                     QAAALFGG"
BASE COUNT          515 a          649 c          597 g          379 t
ORIGIN      
        1 cgcagacggt cgggcagaac cgcgggagcc tcgggatacg ccatggtgcg atatccctgc
       61 ttcagacgcg ctaagatggt ttgcagcata atcggctcct agagatcgtg accgcagtat
      121 gaaaggttaa aactcttgtt acagagtggg aaatccgaga tctgctgtcc ccgtaacgcc
      181 atcgccagcc ccatccagtt atggaatgag ggatcaacga ccttgtacca gagaaagcgt
      241 ccttccgcgt cggtcatggc cgcgtgacag actgctccac gccatccttc ggtgagcgag
      301 acgaccaccc gtcccggttc ggggttttga cactcgccca caatcggggt ggcaggcaga
      361 gatcgcagca tggtcttgat aaatccctcg gaacgttgga tctcaagcca acgcacgtag
      421 gcacgaccga acacatcgcc ggtcgaccag gccgacaccg gaatctgatg aaactggaag
      481 attccacgcg gaaaatcctt gcgcacatca tgttcgattc cactcgcccg cccggccacg
      541 cctaccaggc ccagatctcg cgccattccg agtggtactg ttcccgtctg ttcgaaacga
      601 gctaaaacag aagaggaatc ccacagcagt tgaatggcat tttcggtatc gatcacaacc
      661 tcatggaacc gatcggagaa cttcttcagc cgttcagcgt ccagatcgaa acgcacgcca
      721 cccggggtca gcaggttacg accgaatcgg ctgccacaga tcatggcgga caggttgaga
      781 aagtcaccgc gcagccgccc acaataggaa gcggtcggca gaaagccgac gtcgttggca
      841 agcgcgccca gatcaccgac gtgatttgcc agacgttcga gttcgagcgc aattcctcgc
      901 aagacatggg ctctggccgg gacttttgcc gctcccaggg cttcacgcag ttggacatag
      961 gcaagtccgt gtccgatcgt cgtatccccg gccagtgttt ccatcagaag ctgggtgcgc
     1021 ggtgtcgggc caccccgcaa ggcccgttca ataccgcgat gctgatagcc gagtgagatt
     1081 tcgagatgaa acacgttttc cccgtggcac tgaaaccgaa agtgaccggg ttcgataacc
     1141 ccggcgtgaa cgggaccaac cgccacttcg tgcacctcgt ccccctcgac gcggtagaaa
     1201 ttggtgacgc agggcaggag aggctcttcg gcagagcgat cccaggcatc aaagcccgga
     1261 cgataggaac gatggaaccg gatcggcttc agccagggat gaccgagagg acgaagtccc
     1321 cactgttcgg cgatctcacg ttcaaaccag tgcgccgccg gacaatcggg ggtcagtgcc
     1381 ggatactcct cctcgacgac ggccgaagcc agttcgatcg atccacgcga cgggtccgac
     1441 atcacgccaa cgagtcgcag acgcttcagg tcttcagcca cgggacaggc aaacaaggcc
     1501 gagagattcg caccaccatt gacgcggttc cgcagccatt cacgcagttc actgattcgg
     1561 acttcaggca aatcagaggc aggtgccgca agcgcgttcg ccactttcaa aggtttcaga
     1621 gtctgcatga tttaccctcc gaacaaggct gccgcctgct gcatgacggc aagaacccgt
     1681 gtcggcggcg caattcccat aatcaagact ccgagcaaca gaatggtcaa tggtaccatc
     1741 tgcagaatgg attccgcgtg agcctttttt ccaaggcgct ctggtgacgt gccatacgac
     1801 atatgaatga atggcccgac catgcccaca aaaatgacgg ccaacagcag cacatagacc
     1861 actccgagca gcaatcgccc cgacgtcaga atgtactgag ccacgagaaa ctcactgacg
     1921 aaggtgccga acggaggcgt tccggtaatg gccaggaagc cggcaatcca gaagagagcg
     1981 gtccgcggca ggatgtgttt gatcccatgc acttcagcaa tcgctttggt gtgaaacgca
     2041 tcgagaattc tcccggccga gagaaacagc aaccccttgg aaagggaatg attgaccgcg
     2101 tgatacatgg ccgcgaagac gcctccgccg cccaccccga
//
LOCUS       DAORHD010000921         2139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64521_length_2139_cov_2.694338, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000921 DAORHD010000000
VERSION     DAORHD010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64521_length_2139_cov_2.694338"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PKO06_17055"
     CDS             <1..726
                     /locus_tag="PKO06_17055"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV71415.1"
                     /translation="GPTVASRTTFMSGNALVAAAQDLRSRLLPLAADLLGRQVKEVEL
                     RDGEAFVLADPSGKRISYQHLVQELYKRRMMPSAFGWYIAPDTSFDQSTGQGNAYFSY
                     SFAATIAEVEVDVEIGEVRVTKVTTAHDLGKAINPQQVEGQIEGGTLQGVGYAITEEI
                     RHDAQGKLLNDAFATYILPTTLDAPDVHAIICEHPYREGPYGAKGFGEVPLMGVAPAI
                     ANAVFNATGKRVRHMPMKPEKLV"
     gene            723..>2139
                     /locus_tag="PKO06_17060"
     CDS             723..>2139
                     /locus_tag="PKO06_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71416.1"
                     /translation="MSSNLDPIDRSLPEDHDSTMPLPEWERVFALVATVLVLFFYTRA
                     CPFPAGTYWDLVSARDFDINLGWTVMPEYLSFLVVQSPASLIGLKAIYHVVFFVLLGM
                     MTVWVFRSREVMPGLLLLAIFAFGMQPLLTLRHLLQLLFLAGFFTAFSGVFLKNAFGV
                     MIVPALAAAGAVGLNTWMLLVFVTCHALFRRGFQWTLVLCGLMGLLIFPESTTNAYTG
                     LMPLAGRFMFAEDQYLLLLLGGIFLVPNLLALPMIDEESFPTMIFYALVTIASLLDPG
                     YGPAFVLTGFFLLSRSLHEMDVLSQNVRIVGVILLAILIHLFLLYSPLGFKLNPTVRL
                     ELGAELEPVLHGREATMRVRTSQIGELAWKGMLNPKIEDLAMIQRFSHVIVKSHGGNF
                     EMEVPDWMIPGKKLPEKAGEPSVPVDLPGKAPDAQVEPEAASLPTSVDVSESQNASRE
                     TGLVFEMPTSEELASSAMQRAQ"
BASE COUNT          393 a          596 c          631 g          519 t
ORIGIN      
        1 ggcccgaccg tggccagtcg tacgacgttc atgtccggga atgcgctggt ggcagccgct
       61 caggatcttc gctcacgcct tctgccgctg gccgcagacc tgctggggcg ccaggtgaaa
      121 gaagtcgaac tgcgcgacgg cgaagccttc gtgctggccg atccctcggg caaacgcatt
      181 tcgtatcagc atctggtgca ggaactgtac aaacgacgta tgatgccttc ggcgttcggg
      241 tggtatattg cgcctgatac atcttttgac cagagcaccg gtcagggcaa tgcctatttc
      301 tcgtacagct ttgcggccac cattgctgaa gtcgaggtcg atgtcgaaat cggtgaagtc
      361 cgggtgacga aagtcaccac ggcgcatgac ctggggaaag ccatcaaccc gcaacaggtc
      421 gaaggacaga tcgaaggtgg aacgcttcag ggcgtcggtt atgccatcac cgaagaaata
      481 cgccatgatg cgcagggcaa gctgctgaac gatgcgtttg ccacctatat tctgccgaca
      541 acgctcgatg ctccagatgt tcacgcgatt atctgtgaac atccctaccg cgaaggaccc
      601 tacggcgcca aaggctttgg cgaggtgccg ttgatggggg ttgctcccgc gattgccaat
      661 gccgtcttta atgcgaccgg aaaacgtgtt cgccatatgc ccatgaaacc ggagaaactg
      721 gtgtgagcag caatctcgac cctatcgacc gttccctgcc tgaagatcat gattccacga
      781 tgccgcttcc ggaatgggag cgtgtctttg cgctggtggc gaccgtgctg gtgctgtttt
      841 tctacacgcg cgcctgcccg ttcccggctg gtacctactg ggatctcgtc tctgcccgcg
      901 attttgatat caatctgggc tggacggtca tgccggagta tctctcgttt ctggtcgtgc
      961 agtctccggc atcactgatc gggttgaagg cgatctacca tgtcgtgttc ttcgtcctgc
     1021 tcggcatgat gaccgtctgg gtttttcgca gccgtgaagt gatgcctggt ctactgttgc
     1081 tggcaatctt cgccttcggc atgcaaccgc tgctgacgct gcgtcatctg ctgcaactgc
     1141 tgtttctggc cgggttcttc accgccttct ccggagtgtt tctcaagaat gctttcggcg
     1201 tcatgatcgt tccggccctg gcagccgcgg gagcggtggg cctcaacacc tggatgctgc
     1261 tcgtgttcgt gacctgtcat gccctgtttc gccgtggatt ccaatggacg ctggtgctgt
     1321 gcggcctgat ggggttgttg atctttcctg aaagtacgac gaatgcatac accggcctga
     1381 tgccgctcgc cggccggttc atgtttgccg aagaccagta tcttcttctg ctgctggggg
     1441 gcatctttct ggtgccgaac ctgctggcgt tgcccatgat cgatgaagaa tcctttccga
     1501 ccatgatttt ctatgcgctg gtcactatcg cgtcactgct tgacccaggc tatgggccgg
     1561 cgttcgtgct gacaggtttc ttcctgctct cacgctctct gcacgagatg gatgtcctgt
     1621 cacagaatgt gcgtatcgtc ggcgtcatcc tgttggccat tctcattcat ctgtttctgc
     1681 tctacagccc gcttggtttc aagctcaatc cgactgtgcg actggaactt ggggcagagt
     1741 tggaaccggt tctccacggc cgtgaagcga cgatgagagt ccgaaccagt cagatcggag
     1801 aactggcctg gaagggaatg ttgaacccca agattgaaga tctggcgatg attcagcgtt
     1861 tctctcacgt gattgtgaaa tcgcacggcg ggaacttcga gatggaagtg cccgactgga
     1921 tgatccccgg caagaaacta ccggaaaagg cgggagaacc gtcggtacct gttgatctgc
     1981 cagggaaggc tcccgacgcc caggttgagc cggaagctgc cagcctgcct acttctgttg
     2041 atgtgtcaga gtcgcagaac gcttcgcgtg agaccgggtt ggtgtttgag atgccgacat
     2101 ccgaagagtt ggcgtcctcg gcgatgcaac gggcgcagg
//
LOCUS       DAORHD010000922         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64568_length_2138_cov_3.289486, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000922 DAORHD010000000
VERSION     DAORHD010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64568_length_2138_cov_3.289486"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..631)
                     /locus_tag="PKO06_17065"
     CDS             complement(263..631)
                     /locus_tag="PKO06_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71417.1"
                     /translation="MELLMDPEAVVPEEVAEAVSVLLLLVFRSGDAVPVAVGSVFRVS
                     DALAVVDAAGFIGVLLEAVWAIVLSVLELVAGAVLTATAAVATLGFGDEAILAVLLAW
                     CVESEAVAEAVCAVRLRCGL"
     gene            618..1532
                     /locus_tag="PKO06_17070"
     CDS             618..1532
                     /locus_tag="PKO06_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71418.1"
                     /translation="MSSSTASAPKTLVASAPATSSGTEPIAASTSPEGEGDTSEANAS
                     SSEATAAEDATETASVEGEPAPEGDAPPEGEEGDEGPKEVANLEEIIEEASGHQNSGN
                     WDNLLTAIEKAGDSADARVLALRLDGLLHQKKLNLERIKGTAETLLQTDSRSPIANYA
                     MGYAFAYGRKPDYTKALTHLAKAKAVRKPYPGASSLYWQIYLKKNWWLLLLLVGGGAG
                     GFTQWKKRKKLQLAGTGAVPPAESAASSEESPTADGTAATAGESATDQSAAPATSDSP
                     PENQSDNPTATDTPVEGENDQGSAPGPK"
     gene            complement(1616..1795)
                     /locus_tag="PKO06_17075"
     CDS             complement(1616..1795)
                     /locus_tag="PKO06_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71419.1"
                     /translation="MKNVELTVEGNILTIKVDLTKEFGASSSGKSIIVATTEGNYAIP
                     GREEKIGLNVYRKTK"
BASE COUNT          521 a          687 c          538 g          392 t
ORIGIN      
        1 ggaactggct gttgatctcc gttgctctgt tgtcaggctt ggtggccggt ctcggcccat
       61 tgccgctgtt cgccgcccac accgaccccc tgcaggtctt acgcgatctg caaccaccgg
      121 acgtcgccta tccctttccg ccagacacga atccgacgtc acccaccgcg acttcgtcat
      181 ctcgcctcaa gccagcggtg aatcctcaat tctctgacct ggctccgcct catgcgccga
      241 accaggtccc ggcttcgctc acctacaaac cgcaacgcaa gcgcactgca cagacggctt
      301 cagccacagc ctcgctttcg acgcaccagg cgagcaacac cgccaagatc gcctcgtcac
      361 cgaagccgag cgtcgccacc gctgcggtcg ccgtcaaaac ggcaccggca accagttcga
      421 gcacactcag cacaatcgcc caaacagctt ccagcagtac gccgataaaa cccgcagcat
      481 caacaaccgc caaggcatcc gatacccgga aaacagaacc aaccgcgacg ggaaccgcat
      541 caccagaccg gaaaaccagt agcagcaaga cagaaactgc ctctgccact tcttcaggca
      601 caaccgcttc tggatccatg agcagttcca cggcctccgc tccgaaaact ctggtggcca
      661 gtgcccccgc cacctcctcg ggaacggaac ccatcgcggc ttccacgtca ccagaaggag
      721 agggtgatac ctccgaggcc aatgcgtctt cgtcagaagc cacagctgca gaggacgcga
      781 ctgagaccgc aagtgtcgag ggagaaccag cacccgaagg agatgctccg ccggaaggcg
      841 aggagggcga cgaagggccg aaagaggtcg ccaatcttga agagatcatc gaggaagcga
      901 gtgggcatca aaacagcggc aactgggaca atctgctcac agccatcgaa aaagcaggag
      961 atagtgctga cgctcgtgtt ctcgcactca gactcgatgg cctgctgcac cagaagaaac
     1021 tcaaccttga acgcatcaag ggtactgccg aaaccctgct gcaaacagat tcgcgcagtc
     1081 cgatcgccaa ttatgccatg gggtatgcct tcgcctacgg ccgtaagccg gactacacga
     1141 aagcactcac gcatttagcc aaagcaaaag ccgttcgcaa gccgtatccc ggagcgagca
     1201 gcctgtactg gcaaatctac ctcaagaaga attggtggct tttgctgcta ttggtcggag
     1261 gcggagccgg tggcttcact cagtggaaaa aacgaaagaa gctgcaactg gctggcactg
     1321 gcgccgttcc ccccgcggaa tctgcggcat cttcagaaga atcaccaacg gcggatggta
     1381 ccgccgcgac tgcaggagaa tccgctacag atcagagcgc cgcacccgcg acaagcgatt
     1441 cacccccgga gaaccagagc gataacccga cagccaccga cacaccggtc gaaggggaaa
     1501 acgatcaagg ctcagcaccc ggaccgaaat aacaacgttt tgcgaaaaag cgaaaaccgc
     1561 aggccagtcg gcctgcagat ttcacttctg aactttgcgg ttcgctgcgc tgaccttact
     1621 tggtcttgcg atacacgttc agaccgatct tttcttcgcg acccggaatg gcataattgc
     1681 cttcggtcgt agcaacgatg atcgattttc ctgaggaaga agccccgaat tctttggtca
     1741 gatcgacttt aatcgtcagg atgttgcctt caacggtcaa ttcaacgttc ttcatgtcgg
     1801 aaacccctcc attggcggtt ttactgattg agcgcggcga agataccata atttatcaat
     1861 aaatgctagt aggttttttc tgactcttgt ataatcagcc atgcttcctt gtctgcatca
     1921 gactcagaac agaatctccc tcatcgcact cgtattctcc gttgcgatgg cactcccgat
     1981 gtccgccacc gccgggcatc cgaccattgc cagcgcaagc gcctttatca gcgacctcct
     2041 gtcggcgcga cgggcaaagc agacttttca ggatctggca gcgcgaggtc aggccgccat
     2101 tcccacgatt caaacgctgt tggctcaccg gaatctcc
//
LOCUS       DAORHD010000923         2136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64655_length_2136_cov_4.882749, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000923 DAORHD010000000
VERSION     DAORHD010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64655_length_2136_cov_4.882749"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKO06_17080"
     CDS             complement(<1..256)
                     /locus_tag="PKO06_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV71420.1"
                     /translation="MKGYLIQIEDATRDNQYYRRVLFTAQHSQLVLMSLKPGEEIGEE
                     VHELDQFIRFEEGEGIVVLDGENHAVSDGFAVVIPAGTRHN"
     gene            complement(401..>2136)
                     /locus_tag="PKO06_17085"
     CDS             complement(401..>2136)
                     /locus_tag="PKO06_17085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HNV71421.1"
                     /translation="PGFAVSYNLEPYGAEEIDVLRGPASFLYGQGSPGGLLNYITKRP
                     TRESLHEVQFLTGNFGRHEGRFDFGGKLNDSDVLSFRLTGLFRESGTQLDQVPNDRIY
                     IAPAVTWQAAARTQITFSAHFQKDQLGSSQALPAEGSLRFNSNGRIPVSRFTGHPDIE
                     KQNRTESSLGYEVRHGLAPDWDVVHKLRYSSTELDGLTTYSNSFGTDKRTVSRFAYGS
                     LGKLNALTIDNQVHGKFSTGPFQHQVLGGLDFQRITVDLRQTFGAATDVDIFNRYDYG
                     APFALPPVFLNQRVTQLQTGVYLQDQVKLYDHWLLTLGGRHDWASDETQDRMTGAPSR
                     SQADRKATGRAALTYLFDNGLAPYISYSTFFLPAIGVNSNGQAFTPETGRQYELGIKY
                     QIPRTRTLLTAALFDLTRENFIQTDPSAFLQVQRGKARSRGLELEGVASFDSGLDVIA
                     SFTLLDNEVRETANPLEKGKRLPQTPGQFGSLWVKYTFSGGSLKGFGIGGGARYTGYT
                     YGDEANTFRVPSYVVGDAAIDYTWKHYRFALNFNNIFNQDQFGCFDRSGSFFCTFGER
                     RTVVGSVTYRW"
BASE COUNT          439 a          613 c          618 g          466 t
ORIGIN      
        1 cattgtgcct ggtcccggcc gggatgacga ccgcaaagcc gtcgctcacc gcgtggttct
       61 ctccgtccag gaccacgatt ccctcgccct cttcgaaacg aataaactga tccaactcat
      121 gaacctcttc tccgatttct tcaccaggct tgaggctcat caacaccagc tgggagtgtt
      181 gtgccgtaaa gagcactcga cggtaatact gattgtcgcg tgtggcatct tcgatttgaa
      241 tgagatagcc tttcatggcg atgctccctt cgatcatgaa tcctctgaac aaggggtcca
      301 agaaggacac tgtaccctgg catcccaagg tggacgagtc aagcgtcacg tatcggcgac
      361 cggacgaccg tcgatgaaca ggtagggtct catgctgcgt ttaccatcga taggtgacgc
      421 ttcccaccac agtgcgccgt tcgccgaagg tgcaaaagaa actgccgctc cggtcgaaac
      481 agccgaattg atcttgattg aaaatgttgt tgaagttcag cgcgaaccga taatgcttcc
      541 aggtgtagtc gatggcggcg tcccccacga catagctggg gacgcgaaag gtgttggctt
      601 catcgccgta ggtatacccg gtataccgcg ctccgccgcc gatcccgaac cccttcaggc
      661 tcccgccgga gaacgtgtat ttcacccata atgacccgaa ttgcccgggt gtctgcggca
      721 gccgttttcc cttttcgagc gggttggcgg tctccctgac ttcgttgtcc agtagggtaa
      781 acgaagcgat gacgtccagc ccggagtcga aacttgccac cccttccaac tccagacctc
      841 gcgaacgggc ttttccccgc tgtacctgga gaaaggcgct cgggtcggtc tggatgaagt
      901 tttcgcgcgt taggtcgaac agtgcggcgg tcaagagagt gcgtgtcctg ggaatttgat
      961 acttgatacc cagttcgtac tgacggcccg tttccggcgt gaaggcctgt ccatttgagt
     1021 tcacgccgat cgccggaaga aaaaaggtcg aatagctgat gtagggagcc aggccgttgt
     1081 cgaacagata ggtcagagcg gcgcggccgg tggctttgcg atctgcctga ctcctgctcg
     1141 gcgcccccgt cattcggtct tgcgtctcat cgctcgccca atcgtgccgg ccgccgagcg
     1201 tcaacagcca atggtcatag agcttgacct gatcttgcaa atagacgccg gtttgaagct
     1261 gagtgacccg ctgattcaga aacacgggag gcaacgcaaa aggggcccca taatcgtaac
     1321 gattgaaaat atcgacatcg gtcgccgctc cgaaggtttg ccgcaagtct acggtgatcc
     1381 gctggaagtc cagtcctccc agcacttgat gctgaaacgg accggtggag aatttcccat
     1441 gcacctgatt gtcgatggtc aacgcattca gtttgccgag cgacccgtag gcgaatcgcg
     1501 agacggtgcg tttgtccgtg ccgaaactgt tgctgtaagt ggtcaggccg tcgagctcgg
     1561 tggaactgta gcggagcttg tggaccacat cccagtccgg cgcgaggccg tgtcgcacct
     1621 cataccccaa ggaggattcc gttctatttt gcttttcgat atcgggatgt ccggtgaacc
     1681 gggataccgg gattctgccg ttagaattga agcgcagtga gccttcggcc ggcaaggcct
     1741 gcgaagaccc gagctgatct ttttgaaaat gggccgaaaa cgtgatctgc gttcgtgccg
     1801 ccgcctgcca ggtgacagcg ggcgcaatgt agatgcgatc gttcggcacc tgatcgagct
     1861 gcgtgccgct ttcgcgaaac aggccggtca accggaagga caacacgtcg ctgtcattca
     1921 gcttgccgcc gaagtcgaac cggccttcgt gacggccgaa gtttcccgtc aagaactgca
     1981 cttcgtgcaa cgattcccgg gtcggtcgct tggtgatgta gttcaacaat ccgccggggc
     2041 ttccctgccc atagagaaat gaggccgggc cgcgcagcac gtcgatctct tccgcgccgt
     2101 agggttcaag attgtagctg accgcgaagc cggggt
//
LOCUS       DAORHD010000924         2134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64797_length_2134_cov_2.932179, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000924 DAORHD010000000
VERSION     DAORHD010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64797_length_2134_cov_2.932179"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PKO06_17090"
     CDS             complement(<1..1371)
                     /locus_tag="PKO06_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71422.1"
                     /translation="MFLADCVSIARRFQRSIRIDTDLHEPKALDGYVATKSSIDVLLA
                     MARHVKEQSQGAFTWTGPYGSGKSSLVVVLGSLLSGTAAMRKAAVEAVGRENAEEIWK
                     SFPVNSGGWQIIPIVGRKERPIQILSEVLEDRLPQTASKKGGWSDKQVIEGFMKLADK
                     KQVKSNGVIIFVDEMGKCLEAAGQEGSDIYIFQQLAESASRSGGRLIVVGILHQAFQE
                     YALQLSREMRDEWTKIQGRFVDLPVNAAGEEQIEMLGRAIVTERKQTVAGQLAEQIAE
                     CVAQQKPSVSKYFGVTLENCWPLHPVVACLLGPISRRRFGQNQRSLFAFLNSAEPRGF
                     QDFLKHAREKDLYTPEQLWDYLRLNLEPSILASPDGHRWALAVEAIERCESQSPDDLH
                     LALLKTVALVELFRGSSGLMATQQLLQESFPGYKKSEVRDALKQLQSWSLIIFKKFQN
                     AYAIFAG"
     gene            complement(1383..>2134)
                     /locus_tag="PKO06_17095"
     CDS             complement(1383..>2134)
                     /locus_tag="PKO06_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HNV71423.1"
                     /translation="IRHWSQVTGILADGDAPNQLKTTPLGQKLFGRHGWDPYMENPAT
                     LWLIHWLMSGRAEKTTWYWAFNYYPNSVFEREHLVKGLEKLVTECRWGKISGSTIKRD
                     VECFVRTYVPKPPSPKTTFEEALESPLTELGLIKPVGKRDGFRFVRGPKSSLGRGVFL
                     FALIDFWKNYSTARTLSFETIAHEPGSPGRVFLLDESDLADRLMQLDQDSKGKFRWSE
                     TAGLKQIIRDIDLDLDTAFQFIDEDFQFRHN"
BASE COUNT          489 a          589 c          558 g          498 t
ORIGIN      
        1 cccggcaaag atggcatacg cattctgaaa cttcttgaag atgatcaaag accagctctg
       61 taactgcttg agagcgtctc tgacctcgga ctttttgtaa cctggaaatg attcttgcaa
      121 tagctgctga gtagccatca gacctgaaga cccacggaac agttcaacca aggcgaccgt
      181 cttcaagagc gcgagatgca gatcatctgg gctttggctc tcgcaacgtt caatggcctc
      241 gacggctagt gcccatcgat gaccgtctgg tgatgccaaa atcgaaggtt cgaggttcag
      301 gcgcaggtaa tcccacagtt gctcgggggt gtagagatcc ttttcgcgtg cgtgtttgag
      361 aaagtcctga aaccctcgtg gttcggcgga attgagaaac gcgaataaac tgcgttgatt
      421 ttgcccaaat cggcgacgtg aaataggtcc cagaagacag gccacaaccg ggtgcagagg
      481 ccagcagttt tcgagagtca ctccaaaata cttagacacc gagggcttct gttgcgccac
      541 gcactcggcg atttgctcgg cgagctgacc ggcaacggtt tgcttgcgtt cggtcacgat
      601 ggcccggccg agcatttcga tttgctcttc accggcggca ttgacgggca gatcgacaaa
      661 acggccttga atcttggtcc attcgtctct catttctcgc gagagctgga gagcgtattc
      721 ctgaaatgcc tgatgcagaa tgccgacgac aatcaaccgt ccaccgctac gagaagccga
      781 ttctgctaac tgttgaaaaa tgtaaatatc cgaaccttct tgccctgccg cctccaggca
      841 ctttcccatt tcatcgacga agataatgac accgttgctt ttaacttgtt tcttgtcagc
      901 aagcttcatg aagccttcga tgacttgctt atcagaccaa ccgccctttt tactcgctgt
      961 ctgtggcaag cggtcttcga gcacctcgct gagaatttgg atcggtcgtt ctttgcggcc
     1021 gacgatcgga atgatctgcc atccaccaga gttgacaggg aaagatttcc agatctcttc
     1081 ggcattctcc ctaccgacgg cttcaaccgc ggcttttctc atcgctgccg tgccgctcaa
     1141 gagcgaccca agcaccacga ccagacttga tttcccactg ccatacggac ccgtccaggt
     1201 aaatgcgccc tgagactgtt ctttcacatg ccgagccatg gccaagagga catcgatgga
     1261 tgatttcgtc gcaacatagc cgtcaagggc cttcggttca tgcaggtcgg tatcgatgcg
     1321 gatcgatcgt tgaaatcggc gggcaataga gacgcaatca gcgagaaaca tagcgagacc
     1381 ccttagttgt ggcgaaactg aaaatcttca tcaatgaatt gaaacgcggt gtccaggtcg
     1441 agatcgatat cgcgaatgat ctgcttgagg ccggccgttt cagaccaacg gaacttgccc
     1501 ttcgaatcct gatcgagctg catgaggcga tcagcgaggt cgctctcgtc gagcagaaag
     1561 acgcggccag gcgagcccgg ttcgtgtgca atcgtttcaa aagagagtgt tcgtgctgtc
     1621 gagtagttct tccagaagtc gatcaacgcg aacagaaaga cccctcggcc taaagagctt
     1681 tttgggccac gaacgaagcg gaaaccgtct cgcttgccaa ccggtttgat gagcccgagc
     1741 tcggttagcg gcgactcaag cgcttcttca aaggttgttt tcggtgaggg cggcttcggc
     1801 acatatgtgc gaacgaaaca ctcaacgtcg cgtttgatcg tcgaccccga aatcttcccc
     1861 catcggcact ctgttaccag cttttcgaga cccttgacca agtgctcacg ctcgaacacc
     1921 gagttcggat agtagttgaa ggcccaatac caggtcgttt tttcggcgcg accgctcatc
     1981 aaccaatgaa tgagccacag ggtggccggg ttctccatat acgggtccca gccgtgtctg
     2041 ccgaacagct tctgccccaa gggcgttgtt ttcagctgat tcggcgcatc gccgtctgcg
     2101 agaattcccg tgacttgact ccagtgacgg atcg
//
LOCUS       DAORHD010000925         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64850_length_2133_cov_2.872955, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000925 DAORHD010000000
VERSION     DAORHD010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64850_length_2133_cov_2.872955"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PKO06_17100"
     CDS             complement(<1..1107)
                     /locus_tag="PKO06_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71424.1"
                     /translation="MRRLIPVVLCLLAMILTAHGAVWGADKGPLTVTHQFAEQGDLQP
                     ANWRLTLSFSRQVGLLDLTRQLSYSVKGKNRLLRITSLANNPSVASGSRFTVEPKDDV
                     DTTASVTLTLAATLPAADGPGTLGTPLKLEFSISPFVFISDVRSNYESTRERPRVVHF
                     RSSADLDASEFKQVLKILPPPGKLVFEKSGVEKEYAISGPFITGKKYQLVVPPTQIGN
                     DGPRLQPATFEFVGQGPAPEVSFSSIRSVIELHGRQHLPVQMINTNQVQCRLTRIPPF
                     FAPELASLTTQMKSDLKKGRRAEGDDDAWNVPGTSEQNRAEDQLVDVTQRLEAVKKLS
                     QSGQAGADLLNAFLGDLDQKGEVFFNETLPDKTAR"
     gene            1247..>2133
                     /locus_tag="PKO06_17105"
     CDS             1247..>2133
                     /locus_tag="PKO06_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71425.1"
                     /translation="MTMPSLWRRLSPLSGWVIPVILSLFPVLLGFWDIHTDRQRELEE
                     KRQESLERTNQAAQQLKLCSDLTFWARETSRLLRRDVEDFLNASPDESLTTACSRAMR
                     DPELKGLPPCRIWAVEFPQGDYRQRGQVCIGPGLESGYKPVVQVVLPALARQHVEPHL
                     PLHPRDEMPRLRVMFGDMILSDLFTRDRRGQPFHTILLHSYALATWDLIFREGKPVGG
                     FLLMFPIPADPGRLAQHLTFRNWRRYFRLPQLACAFLPFPHEPGPASGAIVCHPRLRQ
                     SDVFPHLEDLMRRQEIRPNA"
BASE COUNT          420 a          644 c          608 g          461 t
ORIGIN      
        1 gcgtgcggtc ttgtcgggca aggtctcgtt gaagaagact tcgccttttt gatcgagatc
       61 accgagaaaa gcgttcagga gatcggcccc tgcctgtcct gactgcgaga gcttcttcac
      121 cgcttcgagt cgctgcgtca cgtcgacgag ctggtcctcg gcgcgattct gctcagaggt
      181 gccgggtacg ttccaggcat cgtcgtcacc ttcagcgcgg cgccctttct tgaggtcact
      241 cttcatctgc gtggtcaggc tggccagctc aggcgcaaag aacggtggta tgcgcgtcag
      301 gcggcactgc acctgattcg tgttgatcat ctgcaccggc aggtgctgac gaccatgcag
      361 ctcgatgacc gagcgaatgg agctgaagga aacctcggga gcggggccct ggccgacaaa
      421 ctcgaaggtt gccggctgca aacgcgggcc gtcgtttccg atctgagttg gaggaactac
      481 caactggtac tttttaccag taatgaatgg cccagagatg gcgtactctt tttcgacccc
      541 ggacttctcg aagaccagtt ttccgggggg gggcagtatc ttcagaacct gtttgaattc
      601 cgaagcatcg agatcagccg agcttctgaa gtgcaccacc cgaggacgct cacgggtact
      661 ctcatagttc gaacgaacgt cactgatgaa cacgaacggg ctgatggaaa attcgagttt
      721 cagcggcgtg cccaaagttc cagggccgtc ggcggccggc agtgtcgccg ccagagtcag
      781 cgtcacgctt gccgtggtgt cgacgtcgtc cttgggttct accgtaaaac gcgagccaga
      841 ggcgacactg ggattattcg ccaagctggt aatgcgcagt aagcggtttt tccctttcac
      901 cgagtaggac aattggcgtg tcaaatcgag caacccgacc tggcgcgaga aactcagcgt
      961 cagtcgccag tttgccggtt gcaggtcacc ctgttcggcg aactgatgcg tcaccgtcaa
     1021 cggccccttg tccgcgcccc acactgcacc gtgcgcagtc agtatcatcg ccagcaaaca
     1081 cagcaccacc ggaatcaatc gccgcatgtg aacctcctcc acccttcttc attctcatca
     1141 actgaaacga agtatacccc cgtgcagagt cgggggcaag gcaagctgaa actcaagctc
     1201 aggcgttgtg ataccatcga aggaagatga caaaagtgaa tcacccatga ccatgccatc
     1261 gctgtggcgc cgcctttccc cgctgagcgg ctgggtcatt cccgtcattc tgagcctgtt
     1321 tcccgtactg cttgggttct gggacattca caccgatcgc caacgggaac tcgaagaaaa
     1381 gagacaggag tccctggaac gaaccaatca ggcggctcaa cagctgaaat tgtgcagtga
     1441 tctgacgttc tgggcgcgag agacatctcg cctgctgcgc cgcgacgtgg aagattttct
     1501 caacgcgtcg cctgacgaga gtctcacgac ggcctgcagc agggcaatgc gcgatcccga
     1561 actcaaggga ctgccgccct gtcgcatctg ggcggtcgag tttccccagg gcgactatcg
     1621 ccagcgcgga caagtctgta tcggcccggg tctcgaaagc ggttacaagc cggtggttca
     1681 ggtcgtgctt ccggccctcg cacgccagca tgttgagccg catctgccgc ttcatccccg
     1741 agacgagatg ccgcgcctgc gcgtcatgtt cggtgacatg atcctctcgg atctgttcac
     1801 acgtgacagg cgcggtcagc cgtttcacac gattctgctg cactcgtatg ctctggccac
     1861 ctgggatctc attttccgtg agggaaagcc ggtcggcggt tttctgttga tgtttccgat
     1921 tcccgctgat cccggtcgtc tggcgcagca cctgaccttt cgcaactgga gacgctattt
     1981 ccgattgcct caactggcgt gcgcgtttct tccctttccg catgagcctg ggccagcttc
     2041 gggcgctatt gtctgccatc cccgcctacg gcaatccgat gttttccccc acctggaaga
     2101 tctgatgcga cgacaggaaa ttcggccgaa cgc
//
LOCUS       DAORHD010000926         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64879_length_2132_cov_4.954261, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000926 DAORHD010000000
VERSION     DAORHD010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64879_length_2132_cov_4.954261"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2132)
                     /locus_tag="PKO06_17110"
     CDS             complement(<1..>2132)
                     /locus_tag="PKO06_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011123348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNV71426.1"
                     /translation="GQRPIIFYSNGYEHFIWDDTRYPPRPIQGFYKKSELELIIQRRI
                     SRKEPATTPVKSEIVERYYQTRAIRRISEAFSRDRDRKALVVMATGAGKTRTVIALCE
                     LLMRCNWVKRVLFLADRVALVKQAVNAFKTFLPDASPVNLVTEKDGEGRVFVSTYPTM
                     MGLIDETRDEQRRFGVGHFDLVIIDEAHRSVYEKYRAIFEYFDSLLVGLTATPKEDIN
                     RDTYRLFDLEKGVPTDAYPLAEAVKDKFLVPARSVSVPLKFQREGIAYDDLSDEEKEK
                     WDLIEWGDEEERRERVEAAEVNKWLFNADTVDKVLELLMTRGQKVAGGDRLGKTIIFA
                     KNHLHAEYIAERFNAHYPKYCGEFARVIDFQVEYAQSLIDAFSQADKPPHIAISVDML
                     DTGIDVPEVVNLVFFKLVRSKTKFWQMVGRGTRLCPNLFGPGQHKLFFSIFDFCQNLE
                     YFSENPETTDGAAGEPLSKRLFKSRVELIGELDDRILTQGGVPVTEVTELPASFDEPK
                     TDWQVRQSTVLVLQREIASMNLDNFVVRPKRRFVEKFSNPANWMVLSPEARQELAAEV
                     AGLPSGIAPEEEEAKRFDLLMLSLQLANLRAEPAFERLREQVKTIAGLLEEKKVIPLI
                     QAQLPLILDLQTDEWWQDITTPLLEKARRRLRSLVKLIEKGKRVIVYTDFEDESSEGT
                     EIALPEFAGSQGLERFRAKARAFLTTHL"
BASE COUNT          421 a          602 c          608 g          501 t
ORIGIN      
        1 ctcagatgag tggtgagaaa cgcccgcgcc ttggcgcgaa acctttcaag accctgactg
       61 ccggcgaact ccggcagtgc aatctcggtg ccttcactgc tttcgtcctc gaagtcggtg
      121 tagacgatca ctcgtttgcc tttctcgatc agcttcacca gtgaccgcag gcgtcttctc
      181 gctttctcca gcaacggagt cgttatgtcc tgccaccatt catctgtctg cagatcgaga
      241 atcagcggca gctgggcttg aatcagggga atgactttct tctcttccag aagcccggcg
      301 atagtcttca cctgttcccg taaccgctcg aatgctggtt cggcgcgcag gttcgccagt
      361 tgcagagaga gcatcagtag gtcgaatcgc ttggcctctt cttcttcggg ggcgatcccg
      421 gagggaagcc cggctacctc ggcggccagc tcctgtctcg cttccgggga aagtaccatc
      481 cagttcgcgg ggttggaaaa tttctcgacg aaacggcgtt tgggccggac aacgaaattg
      541 tccaggttca ttgaggcgat ttccctctgc aggactaata ctgtactctg tcgaacctgc
      601 cagtcggttt tgggttcatc gaagctggcg gggagttcgg tcacctcggt gacgggtaca
      661 ccgccttgag tgagtattcg gtcatcgagt tcaccgatca gctcgactcg cgatttgaaa
      721 agtctcttgc ttagcggttc cccagcagcg ccatccgtgg tctccgggtt ctcgctgaag
      781 tattcaaggt tttggcagaa gtcaaaaatc gagaagaaca gcttgtgttg tcctggcccg
      841 aacaggttcg ggcacaggcg ggtgccgcgc cccaccatct gccagaactt ggttttcgat
      901 cgaaccaact tgaagaagac caggttgacg acctcgggca cgtcgatgcc ggtatcgagc
      961 atatcgaccg agatcgcgat gtgcggcggc ttatcggcct gtgaaaaggc atcgatgagg
     1021 ctttgcgcat actcgacctg aaagtcgatg acgcgggcga attcgccgca atacttcgga
     1081 taatgggcat tgaagcgttc ggcgatgtac tcggcatgca gatggttctt ggcgaagatg
     1141 atggtcttgc ccagtcgatc gccgcccgcc actttctggc ctcgggtcat cagcagttca
     1201 agcaccttgt cgacggtgtc ggcattaaag agccacttgt ttacctcggc ggcttcgact
     1261 cgttcgcgcc gttcctcctc atcgccccat tcgatcagat cccatttctc tttctcttca
     1321 tcggaaagat cgtcatacgc gatgccctcg cgttgaaact tcaagggtac cgacactgat
     1381 ctggccggaa ccagaaactt gtctttcacc gcttctgcga gcggataggc gtctgtcgga
     1441 acaccttttt cgagatcgaa caggcgatag gtgtcgcggt tgatatcctc tttcggcgtc
     1501 gccgtcaggc ccaccaggag cgagtcgaag tattcgaaaa tcgcccggta cttctcgtag
     1561 accgaccggt gggcttcgtc gatgataacc agatcgaagt ggccgacgcc gaagcggcgc
     1621 tgttcatctc gagtctcgtc gatcagaccc atcatggtcg ggtaggttga aacgaagacg
     1681 cggccttcgc cgtctttctc ggtgaccagg ttgacgggcg acgcgtcagg caggaacgtc
     1741 ttgaacgcgt tgaccgcctg cttgaccagg gcgacgcgat cggcgagaaa caacacccgt
     1801 ttcacccagt tacagcgcat caacagttca cacagcgcga tcacggtgcg ggtcttacca
     1861 gcgcccgtgg ccatgacgac gagagccttg cggtcacgat ctcgggaaaa ggcttcgctg
     1921 atacgtctga tggcgcgggt ctgatagtaa cgttcgacga tctcgctctt caccggagtt
     1981 gtcgccggct ctttgcgcga tatgcggcgc tgaatgatca gttccaactc cgactttttg
     2041 tagaacccct gaatcggtcg gggggggtaa cgggtatcgt cccagatgaa atgttcgtag
     2101 ccgttggaat agaagatgat cggacgttgc cc
//
LOCUS       DAORHD010000927         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_64886_length_2132_cov_3.788637, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000927 DAORHD010000000
VERSION     DAORHD010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64886_length_2132_cov_3.788637"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1209..>2132)
                     /locus_tag="PKO06_17115"
     CDS             complement(1209..>2132)
                     /locus_tag="PKO06_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71427.1"
                     /translation="YHDHDNGDDAMAGGKLNWQRSEKEERLARYGGVKVDDESIIRDE
                     KLSDTKGTTTDKMVQCPHCSWRVQSLLLNEHLLESHGIVGNPFRCFVSAYREKHPRSL
                     ADLFVKDMEERWLARPKDGVLKSMIVELMYRNSNAAKALAAMALTSYLAKQGIDYLVD
                     AESATFCVMSRRPPGRALMSRDFRAVIEWFDTEWEKGRFKKTTAAAIVSPPQRIAEKP
                     VQKPVVTATISRPPVAPPAPKPASNLHREQVAKLMTYQDKPVKGGMKIDIETNANGTF
                     TVRRVVPGFKDLQCANASVEAALAVVERLVR"
BASE COUNT          460 a          632 c          574 g          466 t
ORIGIN      
        1 accccaaccg gcgaatcgag cgtaaacttg tagagcgact tcttgtaaaa aatcgccttt
       61 tcccccatgg agccgctcag ggaaatgtta tgaaacgcct caatcgtcgc gttcggtcta
      121 accgtcgcgc ccgttcgttg cggtattctg acaagtgcta cggtcataga cctgactcgc
      181 agtacgttgt ttgtcgaaga atagaccgca gactgggcaa cagccgatga agtacgcgcc
      241 gttagaatac gagtcaggat ctggcaccgt gaatgacgtt aggatctgaa acacccagga
      301 agcacgcagg aaccgccaga aatcggcagg ctccaccgtc gaagggttta cacgccatga
      361 gaaagtggta tggttgaacg tatcccacga gatctgcatg ccaagtgaat cccggatcgc
      421 ttcttccgcg tcgacttcga gggctacagc ctcttcacgc gtcagatttg ccacccctgg
      481 gccaagatca gtgttgttcg ttgcggcggc cctcaacaaa cgagtcgcaa gtcgaatagt
      541 ctcgaaaaag tgcccctgag cgccctcagc cgcccggacc aactccacct ggtcagcttc
      601 gacaccgcga tccttttcgc gcagaaactc gatgtaatct gtgacaggat ccctcgggac
      661 agtatgctct cgatgttgaa tgtatcgatt cagaaactcg aaaaacttct gcacgtctct
      721 cgtcgcagcc gtaggcacac tacgcctgtc tgggcggggg gcttcacact cagcacgatg
      781 tgagtgtaga acggcaagga tcagctgatc aaacgtcaca ttttctcgac ggcggcgata
      841 gtaatcgagc agagaagctg gtatctgcag ggttacatcc tcaaacgtgt cttcccgata
      901 aatctcgcgg atgacggact gtgagcgcag tctgaccata gttcacctcg tagccggatg
      961 gagtctcgat gcaggctcgg cattctacca cgcaagggcc gaaagcctcg caatccgctc
     1021 acagaaaggg ttatacggcg agatgagccg agcgagtgga agcagtgacg gcgcattctg
     1081 aactcttccc tggcaggaag aagaaaacca cttttccagc gagaagattt acaaagtgct
     1141 tccactgctt ccatccccgc tctgggtaag ggcttgagcc tccacaaaac ggagcccacc
     1201 cgcccccact accgcaccag ccgctccacc acagcgagtg cagcttcgac ggaggcgttc
     1261 gcacactgca gatccttgaa gccgggcaca acccgtcgaa cggtgaaggt tccgttggcg
     1321 ttcgtctcga tgtcgatctt catgccgccc ttcacgggct tatcctgata cgtcatcagc
     1381 ttggcgactt gctcacgatg caggttcgaa gccggtttcg gggccggtgg cgcaacggga
     1441 ggtcggctga tcgttgcggt gacaaccggc ttctgaaccg gtttctcggc tatcctttgt
     1501 ggtggtgaga caatggcggc agcagtggtc ttcttgaacc ggcccttctc ccactccgtg
     1561 tcgaaccatt cgatcacggc gcgaaaatcg cggctcatca gggcacgtcc tggtggtcgt
     1621 cggctcatga cgcagaacgt tgccgactcg gcatcaacca ggtaatcgat gccctgcttc
     1681 gccaggtatg acgtgagagc catggccgcc agcgccttcg ccgcgttgct gttgcgatac
     1741 atgagttcga cgatcatcga cttcagcacg ccgtccttgg gccgtgccag ccagcgctct
     1801 tccatgtcct tcacaaataa atcggcgaga gaccgtgggt gtttctctcg atacgcgctt
     1861 acgaaacagc ggaacgggtt gccgacgatg ccgtgtgatt caagaagatg ctcattcagc
     1921 agcagcgatt gcacccgcca ggaacaatgc gggcactgaa ccatcttgtc cgtcgtggtt
     1981 ccctttgtgt ctgacagctt ctcgtcacga atgatcgact cgtcgtcgac cttgacgcca
     2041 ccgtatctgg ccagccgttc ttccttctca gatctctgcc agttcagctt cccgcctgcc
     2101 atcgcatcgt ctccgttgtc gtggtcatgg ta
//
LOCUS       DAORHD010000928         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65031_length_2129_cov_4.574735, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000928 DAORHD010000000
VERSION     DAORHD010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65031_length_2129_cov_4.574735"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PKO06_17120"
     CDS             <1..437
                     /locus_tag="PKO06_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71428.1"
                     /translation="SSPYLGSSSPDLGSSSPDLKSSSPNLDENRDADGCLIANQLSLP
                     IIDDLSRLSPHLRKELEEFAAEPRTKRKVDRETLRQAILHLCLRHFVTLRALATLVQR
                     KPETLRDEYLTKLVRERKMSLAFPTVPTHSMRAYRTVPGALK"
     gene            434..>2129
                     /locus_tag="PKO06_17125"
     CDS             434..>2129
                     /locus_tag="PKO06_17125"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNV71429.1"
                     /translation="MTNAFTESVVEEAAISWFKELGYAFAHGPELAPGEPASERDSFK
                     DVVLTGRLRDAISKLNPSIPAEAREEALRKILRHESPSLAANNRRFHANLRDGIEVEY
                     RRPDGSIAGDRVHLVDFDNPEENDWLIVNQFTVVEGQHNRRPDLVVFLNGLPLAVIEL
                     KNPADENATIWTAYNQIQTYKNQIGSLLQFNEILVVSDGVQARVGSLTANQEWFKVWR
                     TIEGRQNEPVSALELETLIKGVFQPRRFLDLLQHFIVFEADSDSDRLHKIIAGYHQFH
                     AVNAAVEETVRASGMSEVEVLRDESGAYWAGRMHGGKTGDRRAGVVWHTQGSGKSFSM
                     LFYAARIIRHPVMKNPTLVLLTDRNDLDDQLFGQFQRCQEILHQLPVQAESVEHLRAL
                     LKVASGGVIFTTIQKFMPENKGERVTLLSDRRNIVVIADEAHRSQYDLIDGLARNLRD
                     ALPNASFIGFTGTPIEQTDANTRAIFGDYISIYDIQRAVADGATVPIYYESRIAKLSL
                     NQAELPLIDEEFEEITEGEEDEKKQKLKTKWAALEALVGDPKRIALIAADLVQHYEC"
BASE COUNT          472 a          637 c          597 g          423 t
ORIGIN      
        1 cgagctcccc ttatttgggg tcgagctccc cggatttggg gtcgagctcc ccggatttga
       61 agtcgagctc cccgaatttg gatgaaaata gagatgccga cgggtgtttg atagcaaatc
      121 agctctcact gccgatcatt gatgatctga gccgcttgtc gccccatctg cgtaaagagc
      181 tggaagagtt cgcggctgaa cctcgaacaa agagaaaggt tgatcgtgaa accttgagac
      241 aagccattct tcacctgtgt ttgaggcatt tcgtgaccct gagagcactg gcgactctcg
      301 ttcaacgaaa acccgagacg ttgcgagatg agtacttaac caagcttgtc cgagagagaa
      361 aaatgtctct ggccttccca accgtaccga ctcattctat gcgagcctac cgaaccgtgc
      421 cgggtgcctt gaaatgacca acgctttcac cgagtctgtc gttgaagagg cggccatctc
      481 ctggttcaag gagctggggt atgcgttcgc gcatgggccg gaactggccc ccggggagcc
      541 ggcctcagag cgcgactcgt tcaaggatgt cgttctgacc ggccgactgc gcgatgcgat
      601 cagcaaactg aacccatcga ttccggctga ggcccgcgaa gaagccctgc gcaagattct
      661 gcgccacgag agcccgtcgc tcgcggcgaa caaccgccgg ttccatgcca atctgcgaga
      721 cgggatcgag gtcgagtacc gtcggcccga cggcagtatt gccggtgatc gggttcatct
      781 cgtcgacttc gacaaccccg aagagaacga ctggctcatc gtcaaccagt tcaccgtcgt
      841 cgaaggccag cacaaccgtc gccccgatct ggtcgtcttt ctcaacggtc tgccgctggc
      901 ggtcatcgaa ctcaagaacc ccgccgacga gaacgccacc atctggaccg cctacaacca
      961 gattcagacc tacaaaaacc agatcgggtc gctgttgcag ttcaacgaga ttctcgtcgt
     1021 ctctgacggc gttcaggccc gcgtcggctc tctcaccgca aaccaggaat ggttcaaagt
     1081 ctggcgcacc atcgaaggtc ggcagaacga gccggtttcg gcgctcgaat tggaaacgct
     1141 gatcaaaggt gtgtttcagc ctcgtcgatt cctggatctg cttcaacact tcatcgtctt
     1201 cgaagcagac tcggactcgg accgactgca caagatcatc gccgggtatc accagttcca
     1261 tgccgtgaac gccgccgtcg aagagacggt acgagccagc ggcatgagcg aggtcgaggt
     1321 tctgcgtgac gaatcaggag cgtattgggc gggtcgcatg catggcggca agaccggaga
     1381 ccgacgcgcc ggggtggtct ggcacaccca gggttccggg aagagtttct cgatgttgtt
     1441 ctacgcggcc cgcatcattc gacatccggt tatgaagaac cccactctgg tgctgttgac
     1501 cgaccgcaac gacctggacg atcaattgtt cggacagttc cagcggtgcc aggagattct
     1561 gcatcaactg cccgtacagg ctgaatccgt cgaacacctg cgcgccctgc tgaaagtggc
     1621 cagcggcgga gtgatcttca ccaccattca gaagttcatg cccgaaaaca aaggcgaaag
     1681 agtgactctg ctctctgatc gccggaacat cgtcgtcatc gccgacgaag cgcaccgcag
     1741 tcagtacgat ctcatcgatg gtctggctcg taacctgcgc gacgcccttc ccaacgcttc
     1801 tttcattggc ttcaccggta ctcccatcga gcagaccgac gccaacacgc gcgccatctt
     1861 cggcgattac atcagcatct acgacattca gcgtgccgtc gctgacgggg ccacggtgcc
     1921 gatctattat gaaagccgca ttgccaagct gtcgctcaac caggctgaac tgccgctgat
     1981 cgatgaagag tttgaagaga tcaccgaggg tgaagaggac gagaagaagc agaagctcaa
     2041 gaccaagtgg gccgctctcg aagcgctggt tggcgacccg aaacgcatcg ccctgatcgc
     2101 cgccgatctg gttcagcact atgagtgtc
//
LOCUS       DAORHD010000929         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65055_length_2129_cov_3.357763, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000929 DAORHD010000000
VERSION     DAORHD010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65055_length_2129_cov_3.357763"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..924
                     /locus_tag="PKO06_17130"
     CDS             133..924
                     /locus_tag="PKO06_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71430.1"
                     /translation="MRPLRSTWRWRLCRGLIPALSVLFLPALAPASDLPRTLRESEQW
                     YHTGRLSEARSAALSHHEAFPKDLEALLILAKIDLEAENLIDAKKWLRLADAVDRHHP
                     LVILYRRLLEDLEHRQGPITQMPEPLPTSEKNLTADWFKRNWFWNRPTHHFPKVPIPP
                     RSVPDEFGIATGPTGVLESDSVARQAEDAFRQRQFLRAYLLFSDLVRDHPEDNMYQLG
                     KARAAMALGLYDEARAILAGLQSRYRFTSENPLLSPKVIRFQRQP"
     gene            complement(1122..1964)
                     /locus_tag="PKO06_17135"
     CDS             complement(1122..1964)
                     /locus_tag="PKO06_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71431.1"
                     /translation="MSDTPIGFNMGVPVMDIKKMGLIGSTVLVMASLGMAFSNTPTAA
                     KPATNKAVRVATPVIPATDEALTVVPPAPVAANGQTTPAAESKAVTVSPETKLGLVAP
                     PAVMPKAGKKSGVKAVEAGAVAAKPAMAKPGVKTAAKNGPKAQSAQAGVAKPKNLFSY
                     VRTLSYSPHPQEEEFLREKPLTLNGSEYYISVAYREKWDALDDEDGRIKAVGFDINVY
                     QDGKKVRTLKVPEFVLKPGKMQKGQVIGLAEVAPYKFKIAIYDFTREDGGFSDFTFKF
                     DMSS"
BASE COUNT          403 a          680 c          557 g          489 t
ORIGIN      
        1 cccgacaagc ccgtcgcgtc gctgtctcaa cccgatacgt ccgactggga cagtctgaac
       61 gcttcgggaa ctccgtgaca cgctcgtgac acgaacgggg tcacccgatc acccgaaagc
      121 cggagtcgcc tgatgaggcc tctgcgttcc acctggcgct ggcgtctctg tcgcggcttg
      181 attcccgcat tgtcggtgct cttcttgccg gcgctggctc cggcctccga tctgcctcgc
      241 acattgcgag agtctgaaca gtggtatcac acgggtcggc tctccgaggc gcgctccgcg
      301 gccctttcgc atcatgaggc ttttcccaaa gatctcgagg ctctgctgat cctggccaag
      361 atcgatcttg aagccgagaa cctgattgac gccaaaaagt ggcttcgact ggcagatgcc
      421 gtcgaccgtc accacccgct ggtcatcctg tatcgacgtc tgctggaaga tctcgagcat
      481 cgccagggac cgatcacaca aatgcccgag cccctgccca cctccgaaaa aaacctgacc
      541 gcggactggt ttaaacgcaa ctggttctgg aaccggccaa cgcatcattt tccaaaagta
      601 ccaattcccc cacgcagtgt tcctgatgaa ttcggcattg ccaccggacc aaccggagtt
      661 ctcgaatcag attcggttgc ccgacaggcg gaagatgctt ttcgacagag gcagtttctc
      721 cgtgcgtatc tgcttttctc agatctggtg cgtgatcacc ctgaagacaa catgtaccag
      781 ttgggaaagg cccgtgccgc catggcactt ggcctgtatg atgaagctcg cgccattctc
      841 gccggcctgc agagccgcta tcggttcacg tccgagaatc ctctcttgtc tcccaaggtt
      901 attcgcttcc agcgacaacc ttaaccgaga cccacacgga caatcgcaca taccggcacc
      961 ttcgactccc ctgatcgtca aggcttcgcc ggccgattcc gccatagccg tttgatgttg
     1021 ccgcccgttc tttcggcttc tttcacgacc agaacgcaga cggcgcgggc atcatggcga
     1081 tgctcgcgcc gtctgaacgt ttcgacgtcg tgtcgacgag atcaggagga catgtcaaac
     1141 ttgaaggtga agtctgagaa cccgccatct tcgcgagtga agtcatagat ggcaatcttg
     1201 aacttgtacg gagcgacttc cgccagtccg atcacctgcc ctttctgcat cttgccgggc
     1261 ttcagcacga attcaggcac tttcaacgtg cgaaccttct tgccgtcctg atacacattg
     1321 atatcgaacc cgaccgcttt gatgcgtccg tcctcgtcat ccagagcatc ccacttttcg
     1381 cggtaggcga cggaaatgta atactcgctg ccgttgagcg tcagcggctt ttcacgcaga
     1441 aactcctctt cctgcggatg cgggctgtag ctcagggtgc gcacgtatga aaacagattc
     1501 ttgggcttgg cgacgccggc ctgggcactc tgagctttcg gtccattctt ggctgcagtc
     1561 ttcacgccag gtttggccat cgccggtttc gcggcaaccg cacccgcttc gaccgccttc
     1621 acaccagact ttttccccgc ctttggcatc actgccggag gggcaaccag gccaagtttt
     1681 gtctcgggag acaccgttac cgccttcgat tccgccgcgg gagtcgtttg tccatttgct
     1741 gcgaccggtg ccggtggcac gacggtcaga gcttcatcag tagccggaat aacaggagtg
     1801 gcgacgcgca cggctttgtt tgtggccggc ttcgccgctg tcggcgtgtt cgagaaagcc
     1861 attcccagac ttgccattac cagaactgtc gatccgatca gacccatctt cttgatatcc
     1921 atcactggta ctcccatgtt aaatccaatt ggcgtgtcag acacaccttg ttccggtact
     1981 cgatcagttg ttccgcgctc caccgttgat ccagttggtg atcagcccct gatcatgcgt
     2041 ggccagagcc ggacggctct tgggcatgcg cggcgtctcc acccctttga tgcgacgcac
     2101 cagcttgctg gcttccgcat catacggga
//
LOCUS       DAORHD010000930         2127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65143_length_2127_cov_4.496622, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000930 DAORHD010000000
VERSION     DAORHD010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65143_length_2127_cov_4.496622"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKO06_17140"
     CDS             <1..309
                     /locus_tag="PKO06_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71432.1"
                     /translation="SSYGLDTASLQLASARSASTNAPVRIPEYNSKGEKLYLISNDEA
                     AQIRREAKLSKLVSGSRDQSVRFLEAFNKFLDSYSDEDKASTTTTQEGDNGTSQTTEV
                     "
     gene            623..1534
                     /locus_tag="PKO06_17145"
     CDS             623..1534
                     /locus_tag="PKO06_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71433.1"
                     /translation="MAFLSRWWQFLRERHDPIGISLMVLAFFSANAALTYDPARTPPT
                     PDSLSLFGGFVLIWFLFLHMRLFDEVKDYQFDREHNPERPLARGLISLGEFGTITLLV
                     IISELVLAAHLGWGTFCSFAFTLCFTLLMRQEFFIGDWMRPKLELYAITHTFSASLMG
                     LTIFSIMTGKVPVMIEYPSLLFALSNWFIFNVFEFGRKTFGREEEREGVDSYSARLYP
                     WGAVVLLLINLVAGYGLMMTACQQRFGSFPLSVATPTAIISGIVIFSGLYYSFTPSRF
                     GAKMYRGAVTFFLLAYHVAIGVGVWMR"
     gene            complement(1535..>2127)
                     /locus_tag="PKO06_17150"
     CDS             complement(1535..>2127)
                     /locus_tag="PKO06_17150"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNV71434.1"
                     /translation="PTPQQIKMATHLASEGVDIIFGSHSHVVQPVSWVESPDGARRTL
                     VVWSLGNFISNQRFEFLQRHNTEDGLMVSVRVERVASSSSVQITGIDYQPLWVHRHQK
                     NGRWHYHVLPLLSALRNLDGYGLEQNTNLTRARNSLKRTMKTLAKAPEWSPPTWTENI
                     SDQAVTVKPEKLVTDSSPAPQKTESDDEGSEDEKDE"
BASE COUNT          429 a          639 c          538 g          521 t
ORIGIN      
        1 tccagttacg gccttgacac tgccagcctt cagcttgctt cagctcgctc ggcttccacc
       61 aacgctccgg tacgcatccc cgaatacaat agtaaggggg aaaaactcta tctcatctcc
      121 aatgacgaag cagcgcaaat caggcgcgaa gcgaagctga gcaaactggt gagcggcagc
      181 cgcgatcaat cggttcgctt cctcgaagct ttcaacaagt tcctggactc ctatagtgat
      241 gaagacaaag cttccaccac cacaacccag gaaggcgaca acggaacttc ccagacaact
      301 gaggtctagc cctgctcttg accaggctct gatctacacg tcagctctga cccacacacg
      361 tctcaatcct ccacttccag tcgtttgatt tcgagcacag cagactcctc ctccgaaaac
      421 agaacgtctt tctcacgctg cggggtgtgc gagagactgc gtctcccgag gtcacgatca
      481 atcggaacgc acccgggaaa cggctatgaa cagcgcctga gccagacatg gttcaggcgt
      541 ttttttcgtc ccgaagagga tgtgacagcc ccaaccctga tcgagtagac taccgacacg
      601 aacccaagat caggagcaga gcatggcctt cctttcccgt tggtggcaat ttctgcgtga
      661 gcgtcacgat ccaatcggta tctcattgat ggtgctggca tttttctcgg cgaatgccgc
      721 tctcacctat gatccagccc gaaccccgcc gactcccgac tccctttcgc tgtttggcgg
      781 tttcgttctc atctggtttc tctttctgca catgcgcctg ttcgacgaag tgaaagacta
      841 ccagttcgac cgcgaacaca accccgaacg acctctggcc cgcggcctga tctcactggg
      901 cgagttcggc accatcactc tcctggtcat catctccgag ctggttctcg ccgcgcacct
      961 gggttggggc actttctgtt cattcgcctt cacgctctgc ttcacccttc tcatgcgaca
     1021 ggaatttttc atcggcgact ggatgcgccc caaactcgaa ttgtatgcca tcacccacac
     1081 cttctcagcc agtctgatgg gactcaccat tttcagcatt atgaccggaa aagtaccagt
     1141 gatgatcgag tatccgtctc ttctgttcgc tctcagcaac tggtttattt tcaatgtctt
     1201 cgagttcggc cgcaagacct tcggccgcga ggaagaacgg gaaggtgtcg actcgtattc
     1261 agctcgtctg tatccctggg gagctgtcgt gctcctgctc atcaacctcg tcgccggtta
     1321 cgggctgatg atgaccgcct gccagcagcg gttcggttca tttccgctct ctgtcgcaac
     1381 gcctaccgcg atcatcagcg gcatcgtcat cttttcaggc ttgtactatt catttacgcc
     1441 ctcgcgattt ggagccaaga tgtatcgcgg cgccgtgacg ttctttttgc tggcctatca
     1501 cgtcgccatt ggcgtcgggg tgtggatgcg atagctactc gtctttctca tcttcggagc
     1561 cttcgtcgtc gctctctgtt ttttggggag cgggcgagga atccgtcacc agcttttccg
     1621 gtttcaccgt gaccgcctga tcgctgatgt tttccgtcca ggttggcggc gaccattccg
     1681 gagctttggc aagagttttc atggttcgtt tcagcgagtt gcgagcacgc gtaagattcg
     1741 tgttctgttc gagaccatag ccgtccaggt tgcgaagggc tgaaagcagc ggtagaacat
     1801 gataatgcca gcgaccgttc ttctgatgtc gatgcaccca caaaggctgg tagtcgatac
     1861 ccgttatctg caccgacgat gaggaggcca ctcgttcgac tcgaacactg accatcagac
     1921 cgtcttcggt attgtggcgc tgcagaaact caaatcgctg gttcgagatg aagttgccga
     1981 gcgaccagac gacgagggtt cgacgtgcgc catcgggtga ttcgacccag gataccggtt
     2041 gcacgacatg tgagtgactg ccaaagatga tatcaactcc ctcgctcgcc agatgggtcg
     2101 ccatcttgat ctgctgcgga gtcggct
//
LOCUS       DAORHD010000931         2126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65216_length_2126_cov_3.356832, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000931 DAORHD010000000
VERSION     DAORHD010000931.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65216_length_2126_cov_3.356832"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PKO06_17155"
     CDS             complement(<1..639)
                     /locus_tag="PKO06_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71435.1"
                     /translation="MKRTPPLLRFLALMLLLTSSGVSPLIANEVFSDFSFQTNNDRSV
                     LLFPAHRIPDIMVSRVNSELVFIDIPWFSMDSEPRVMVAPHPKVRFARVSQVSSNPPV
                     VRASVLFYKGGTMNPIVRSGHLYLEDSSTSTAGEISSQDLYSDGMLPNAPLHARASTS
                     TGGSDVLAQRVTLSFDQEDLSTILNALAMKLGLHIFADAGVRGKFTIHANDVP"
     gene            complement(951..1907)
                     /locus_tag="PKO06_17160"
     CDS             complement(951..1907)
                     /locus_tag="PKO06_17160"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HNV71436.1"
                     /translation="MIQVLVLLVVSAIVAYLGDLLGTWVGKRRLTIFGLRPRATAILV
                     AISTGMLITTITLLTAAAISSEVRIALFSVQELMASRQKLTVEVDGLKKEQQKLQTDI
                     STLQERVRSKEQELVVFRRDEPISAMVVNQGTSPASITAELVAFCRTVSRLARQKGLL
                     VKDEETFSLENREQLEKMAVHIASSGEELVVGAIAMQNLTAGEPLGHVNVVVRPNSLI
                     FKNGDSIASLQVDGSLARESIARSLLDFMEEINLEVVRQGMIGNPLTGRFGDLSSESM
                     LSFYDMVQQIRSLNRKLTVVAVVKEDTYAAGPLNVKFKLEQD"
     gene            complement(1921..>2126)
                     /locus_tag="PKO06_17165"
     CDS             complement(1921..>2126)
                     /locus_tag="PKO06_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71437.1"
                     /translation="LQRKGLGLLITDHNVRETLAIVDRAYIMNLGKILVSGDAATVAA
                     DPTARKFYLGERFQLDQIQKDFT"
BASE COUNT          482 a          600 c          624 g          420 t
ORIGIN      
        1 aggcacatcg ttggcgtgaa tcgtgaattt gccgcgcaca ccggcatcgg cgaagatgtg
       61 cagcccgagc ttcattgcca gggcgttcag gatggtcgag aggtcttcct gatcgaatga
      121 gagagtcacc cgctgggcga ggacgtcact gccaccggtg gaagtggagg cacgcgcatg
      181 gagcggagcg ttggggagca tgccgtcgga gtagagatcc tgtgacgaga tttctccagc
      241 cgtcgaggtc gaactgtctt cgagatacag atgaccagag cgaacgatcg gattcatcgt
      301 tccgcccttg tagaacagca cagaagctcg caccaccggc gggttcgagc tgacctgact
      361 gacccgggca aaccggactt tcggatgcgg agcgaccatc acccgaggtt cggaatccat
      421 ggaaaaccag ggaatatcga taaacaccag ttccgagttg acgcgcgaca ccatgatgtc
      481 gggaatgcgg tgcgccggaa acaacagcac actgcgatcg ttgttcgtct ggaaactgaa
      541 gtcgctgaag acctcgttgg cgatcagcgg tgaaacgccc gagctcgtca gcaacagcat
      601 caacgccaaa aaccgcaaga gaggcggcgt tcgcttcata tcttcgctcc tttgaccatc
      661 cggcatcatg gaaccggcaa cagataatga tccaattcct gatatcggca atctgaacaa
      721 agaagttgag tggaaggatt gtcaagggtg atttttcgcg gcaacatgcc gcaagagacc
      781 ggtctcaagg tgcattgccg tgggccggaa tcgccgcgag aaaccatggc ctctctcgaa
      841 acgaggatcc cccaggatca cacattcgag cagcctggaa tcgcagagag agggcaagtg
      901 ctatttctgc actgtgcagc aaccagccac cacggccaat gtcgagaggt tcaatcctgc
      961 tcgagtttaa atttcacatt cagggggccg gcggcgtagg tgtcctcttt gacaacggca
     1021 acgacggtca gcttacgatt cagcgagcga atctgctgca ccatgtcgta aaacgacagc
     1081 atcgactcgg aggacagatc gccgaaacgc ccggtgaggg ggtttccgat catgccctgt
     1141 cggacgactt ccaggttgat ttcttccatg aagtcgagca ggctccgcgc gatgctttca
     1201 cgcgccagcg agccatcgac ctgcaggctg gcaatcgaat cgccattctt gaaaatgagg
     1261 ctgttggggc gaaccacgac gttcacgtga ccgaggggtt cgccagccgt gagattctgc
     1321 atcgcgatag caccgacgac gagctcctct ccggaactgg cgatatgtac ggccatcttt
     1381 tcgagttgtt ctcggttttc gagcgagaag gtttcttcgt ctttgaccag cagccccttc
     1441 tggcgcgcca gacgcgagac ggtgcgacaa aacgcgacca actcggcggt gatcgacgcc
     1501 ggagacgtgc cctgattcac gaccatggcc gagatcggct catcgcggcg gaacaccacc
     1561 agttcctgtt ccttggaacg aacgcgttcc tggagggtgg agatgtcggt ctgcagcttc
     1621 tgctgttcct ttttcagtcc gtcgacttcg acggtcagct tctgtcgcga cgccatgagt
     1681 tcctgcaccg agaacaaggc gatgcgcacc tcgctcgaga ttgccgccgc cgtcagcagc
     1741 gtaatagtgg tgatcagcat tcccgtcgaa atggcaacaa gaatggcggt ggcgcgcggt
     1801 cgcaaaccga agatggtcag tcgccgcttg ccgacccagg ttccgagcag atcgccgagg
     1861 taggcgacga tagcgctgac gacgagaaga acgagaacct ggatcacaag gtcgttcctc
     1921 tcatgtgaag tctttctgaa tctgatcgag ctgaaatcgc tcacccagat agaacttgcg
     1981 cgcagtcggg tcagccgcca ccgtcgccgc gtcacccgaa acgagaatct tgcccaggtt
     2041 catgatgtag gcccggtcaa cgatcgccag cgtttcgcgc acattgtggt cggtgatcaa
     2101 cagtccgaga cctttgcgct gcaact
//
LOCUS       DAORHD010000932         2123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65371_length_2123_cov_2.713733, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000932 DAORHD010000000
VERSION     DAORHD010000932.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65371_length_2123_cov_2.713733"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..>2123
                     /locus_tag="PKO06_17170"
     CDS             273..>2123
                     /locus_tag="PKO06_17170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV71438.1"
                     /translation="MKRREFRELIQHLELLAKQAPTLYRLRLTALALLGYAYLLVILL
                     VLAGVLGLIGILVWAAIVKGKAGAVAIQLLLKLGLPAAIMAYVVLKAFWVRIPAPEGR
                     FLQPHEAPALFRLIEEIRLALDSPPVHAILLTHEFNAGIYQWPRLGVLGWQKNYLILG
                     FPLLACLTPQQFQAVLAHEFGHLSGRHSAFSNWIYRLRSTWGQLLTALVTQENMGGWL
                     FNRFINWFAPYFDAYAFVIGREHEYEADRLSATIVGKETAQQALTAVCAMARFLDEDY
                     WPPLNQLPARQAEPPHNVYSNLLRHLGNGVDQKKAEKWVHEALQLKTDIDDTHPALAD
                     RLAALAGSRLFGLSDLLAPELRLRRPHEPAAGTAYLAEFMETWGQELDAQWFEAVQEE
                     WKKAHEQAQNELKELADLRQKATETALTEDEAWKLVVWTGQHEGDEPARPLLMEFLRK
                     HPDHPWANHTYGRVLLAEKDSRGEGYLTRAMERAHDLVIPGCEALAIYYHQLGRDDQA
                     ELFRERIEAQSEIYQQAQIERNEIAPTDKLLAHGLDTETLTGLAGLVQPHSEIIEGYV
                     ARKSLQHFPDEPCYLVGILVRHPFWTRTFTDTAPKNIELVQQLANDMKLPG"
BASE COUNT          439 a          675 c          607 g          402 t
ORIGIN      
        1 gtcgagcgaa cgaatgccgt agacttccag ctcgcgtacc attcgctgtc gatcttccaa
       61 gcgatcatca ggggcagaca aagcctcgga accggaaatc cgcagagtcg tccacaacag
      121 cagcatgcca gacacaatca ggcttgagcg cagcatcgat cctcctcacc gtatctcatc
      181 tttcatcagt gtatcacagg atggaaggac agggatttac agcgattatc agaacgcgat
      241 actctgcacg gagatcacgc caaggaagcc ccatgaaacg gcgcgagttt cgcgaattga
      301 ttcagcatct tgagctgctg gcgaagcagg ctcccaccct ctatcgtctg cgcctgacag
      361 ccctggccct gctggggtat gcctacctgc tcgtgattct gctggtgctg gccggggtgc
      421 tcgggctgat cggcattctg gtgtgggcgg ccatcgtcaa gggcaaagcc ggtgcagtgg
      481 cgattcagct gctcctgaaa ctcggccttc ccgccgccat tatggcctac gtcgtgctca
      541 aggcattctg ggtgcgcatt cccgcgcccg aaggacgctt tctgcaaccg cacgaagcac
      601 cggcgctctt tcgcctgatc gaagagattc gcctcgccct cgactccccg cctgttcatg
      661 ccattctgct gacccacgaa ttcaacgccg gtatctatca atggcccagg ctgggcgtgc
      721 tcggctggca gaaaaactat ctgatcctgg ggttcccgct gctggcgtgt ctgaccccgc
      781 agcagttcca ggccgtgctg gcccatgaat tcggtcacct ttccggtcgt cacagcgcgt
      841 tcagtaactg gatctaccgc ctgcgatcga cgtggggtca gctgctgacc gctctcgtca
      901 cccaggaaaa catgggcggg tggctgttca accgtttcat caactggttt gcgccctact
      961 tcgacgcgta tgcctttgtc atcggtcgcg agcacgagta cgaggccgat cgtctgtcgg
     1021 cgaccattgt cggcaaggaa acggcacagc aggccttgac tgccgtctgc gccatggccc
     1081 gcttcctcga cgaggattac tggccgcccc tgaaccagct tccggcccgt caggctgaac
     1141 cgccacacaa cgtgtattcc aacctgctgc ggcacctggg caacggggtc gatcagaaaa
     1201 aagccgaaaa gtgggtgcat gaggccctgc agctgaaaac cgacatcgac gacacgcatc
     1261 ccgctctggc cgatcgactc gcggccctgg caggaagccg gttgttcggt ctcagcgatc
     1321 tgctcgcccc cgagctgcgt ctgcgccgtc cgcatgaacc agccgccggc accgcgtatc
     1381 tggcagagtt catggaaacc tggggtcagg agctcgatgc ccagtggttt gaggccgtgc
     1441 aggaagagtg gaaaaaagcc catgagcaag cccagaatga gctcaaggaa ctggctgatc
     1501 tgcgccagaa ggccaccgag actgccctga ccgaggacga agcctggaag ctcgtggtct
     1561 ggaccgggca gcatgaaggc gatgaaccag cccgcccgtt gctcatggag tttctgcgca
     1621 agcatcccga tcacccctgg gccaaccaca catacggacg tgtactgctg gcggaaaagg
     1681 acagtcgcgg cgaaggctat ctcacccgcg ccatggaacg cgcgcacgat ctcgtgattc
     1741 ccggatgtga agcgctggcg atctactatc accagctcgg acgcgacgat caggccgagc
     1801 tctttcgcga acgcatcgag gcccagtcag agatttatca gcaggcccag atcgagcgca
     1861 acgagatcgc tccgaccgac aagctgctgg cacacggcct tgacaccgag accctgacgg
     1921 gactggccgg cctcgtccaa ccgcattcgg aaatcatcga ggggtatgtc gcacgcaagt
     1981 ctctgcagca ttttcccgat gaaccctgct atctcgttgg cattctggtg cgacacccgt
     2041 tctggacccg cacgttcacg gacaccgcgc cgaaaaacat cgaactggta cagcagctgg
     2101 ccaacgacat gaaactgccg ggt
//
LOCUS       DAORHD010000933         2121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65454_length_2121_cov_4.116167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000933 DAORHD010000000
VERSION     DAORHD010000933.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65454_length_2121_cov_4.116167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1099)
                     /locus_tag="PKO06_17175"
     CDS             complement(116..1099)
                     /locus_tag="PKO06_17175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV71439.1"
                     /translation="MEQNLWPMLCKTLESFSSHDTTPRLIQKLFRLMSQHCACHSAAV
                     VFKNPKNDYLEISHSHNIFRDSVHRFQRQVGTRLIGRLFFTDPFVIVTPAQVDDYQEF
                     LIDEPYQLAIGARIATQGRPLGFLVVYFAQPFTVTDPMRSFINSVASLISASIEREEM
                     EDLVRNLRRVDPKTGLLTPNYLLLRLTEEISKARRSKQNLVTALFDIDNFKSIIDRYS
                     LSAAQELYRLVGEELKTCIRGVDVLGLFGTDEYFLYLPNTSVHDADVVIRRFHQSMNT
                     RRFTKDNISTSLSIGITQLAHSDSLETLVQRTQVAFHQARQAGIGQIKILN"
     gene            complement(1238..1516)
                     /locus_tag="PKO06_17180"
     CDS             complement(1238..1516)
                     /locus_tag="PKO06_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71440.1"
                     /translation="MTDNPNPAVPVQEVEQTTNPAPETARKPAVRRKPAPRKSSMLKM
                     VATPEIQARYEAACKRLEGSGEALNREVWEMVKTELESLYEFKTPKAE"
     gene            complement(1513..1941)
                     /locus_tag="PKO06_17185"
     CDS             complement(1513..1941)
                     /locus_tag="PKO06_17185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV71441.1"
                     /translation="MDQARQLGIADRVRHFGWRDDLPTVIGQMDLGLITSTGSEANCR
                     VALEFMASGKPVVATTVGVIPEILVDGEQGFLAAPRAAEPLAQGLARLLNNPALRLRL
                     GRQALEHCRANFGLDLFTHRTEHVYQHALQRRTHILGGSL"
     gene            1940..2065
                     /locus_tag="PKO06_17190"
     CDS             1940..2065
                     /locus_tag="PKO06_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71442.1"
                     /translation="MSQPNDLALASMCHGIELSVCELVGETPRGTPPEVVDYVVT"
BASE COUNT          406 a          591 c          703 g          421 t
ORIGIN      
        1 caagcccgac gaggcgcaga aactgtgtcg cgaagtgctg acgctcaaac ccgatcacgc
       61 gggcgcgaag aaactgctcg agtcgaactg aatcgaccga caccgagctt cacgttcagt
      121 tcagaatctt gatctggccg attccggcct ggcgggcctg atgaaaggcg acctgcgtgc
      181 gctgcaccaa tgtttcgaga gagtccgagt gggcgagttg cgtaataccg atcgatagcg
      241 atgttgagat gttgtctttg gtaaaccggc gggtgttcat gctctggtga aaccgacgaa
      301 ttaccacgtc ggcgtcgtga acgctggtgt tgggcagata cagaaaatac tcgtcggtac
      361 cgaacaaccc gagcacgtcg acgccgcgaa tgcaggtttt gagctcttcc ccgaccaggc
      421 ggtagagttc ctgcgcggca ctcaaactgt accggtcgat gatcgacttg aagttgtcga
      481 tgtcgaacag agccgtgacc aggttctgtt tcgaccgtcg cgccttcgag atctcttcgg
      541 tcagacgcag cagcagatag ttcggcgtga gcagcccggt tttcggatcg acgcgccgca
      601 gattgcgtac caggtcttcc atctcttcgc gctcgattga ggccgaaatg agcgaagcga
      661 ccgagttgat gaagctgcgc atcgggtcgg tgaccgtgaa tggctgcgcg aagtagacga
      721 cgagaaaccc gagcgggcgg ccctgcgtgg cgatgcgggc gccgatggcg agctgatagg
      781 gctcgtcgat gagaaactcc tggtaatcgt caacctgggc gggagtcacg atcacgaacg
      841 gatcggtgaa aaaaagccgt ccgatcagtc gcgtgccgac ctggcgctga aaccgatgca
      901 ccgagtcacg aaaaatgttg tggctgtgcg agatctcgag gtaatcgttc ttcgggttct
      961 tgaagacgac cgcggcggaa tgacaggcgc agtgctgact catcaggcga aacagctttt
     1021 gaatcagccg cggagtggtg tcatgcgagg aaaagctctc gagtgtcttg cagagcatcg
     1081 gccagagatt ctgttccata cgagtctcct ctcgcgcgcg actttttctt ccctctagtt
     1141 tagaaggtag gtcgccgacc gaagaaagtc aacgcgcgtg tgcacctggc gacggcggcg
     1201 atacgccgaa atcatcggcg tgtgcgagtt tgccgggcta ctcggccttc ggggtcttga
     1261 actcgtagag tgattcgagt tcggtcttga ccatctccca gacctcgcgg ttcagggctt
     1321 caccgctgcc ttcgaggcgt ttgcaggcgg cttcgtagcg ggcctgaatc tcgggcgtgg
     1381 ccaccatctt gagcatcgag cttttacggg gggcgggttt gcggcgcacg gccggtttgc
     1441 gcgcggtttc cggagccggg ttcgtggtct gttcgacttc ttgaacggga acggcgggat
     1501 tcgggttgtc ggtcatagcg aacctccgag aatgtgggta cgacgttgca gagcgtgttg
     1561 atacacgtgt tcagtccgat gggtgaacag gtcgagcccg aagttggcgc gacagtgttc
     1621 gagtgcctgt ctgccgagac gcagacgcag cgcggggttg ttcagcaggc gggcgaggcc
     1681 ctgggcgagc ggctcggcgg cccgcggcgc ggcgagaaac ccctgttcgc cgtcgacgag
     1741 aatttccgga atgacgccga ccgtggtggc cacgaccggc ttgcccgacg ccatgaactc
     1801 gagcgcgacg cggcagttgg cttccgaacc ggtcgaggtg atcaggccga gatccatctg
     1861 gccgatgacg gtgggcaggt cgtcgcgcca gccgaaatgc cgcacccggt cagcgatacc
     1921 cagctgacgg gcctggtcca tgagccagcc gaacgacctg gccctggcct cgatgtgcca
     1981 cggcatcgag ctatcggtct gcgagctggt cggcgaaacg ccgcgtggca cgccgccgga
     2041 agtggtcgat tacgtcgtca cctgagccgg ggcgagggca tccggcggtg tgcaacgtat
     2101 aattcgccct tccccgtcag c
//
LOCUS       DAORHD010000934         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65496_length_2120_cov_3.820339, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000934 DAORHD010000000
VERSION     DAORHD010000934.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65496_length_2120_cov_3.820339"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PKO06_17195"
     CDS             complement(<1..752)
                     /locus_tag="PKO06_17195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV71443.1"
                     /translation="MQAPDLRPWLRRLREGGPPEAVVQAFVKILPTCIPGAWLQVQDF
                     PGDARLSELAAFEVETPCGWLFPVAGRWAVSPVDEPWYRSAVQTLLISLEPRAETPDT
                     ANLSESRDHLQALRESEARFRAMVEHFPAGAVLVSGEEILINRAVVAITGYTAAQVPT
                     LSEWFRLLFGDEAVTVRRLWESDRLDGFQRPREVWIKRSDGVRRLVQFHAYGWEDRGV
                     RKDVWLLNDVTERVRAVETLRESENRYRLLAE"
     gene            891..1418
                     /locus_tag="PKO06_17200"
     CDS             891..1418
                     /locus_tag="PKO06_17200"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV71444.1"
                     /translation="MNSPEITPETLLPRLYASIEPFTCRQSCTDCCRGIVSLSIPELQ
                     RIETYFDARGEPLAAQAIGCPFRTPDGCRVYPVRPFMCRLFGYRFLQASLRSQPSCPH
                     LPDERDGEKELQAFTTYQAFCREHGFVLIGESADRLRRSEAVAENQSVLRVFPELTRF
                     RSWLTGGDQLLPAPR"
     gene            complement(1446..>2120)
                     /locus_tag="PKO06_17205"
     CDS             complement(1446..>2120)
                     /locus_tag="PKO06_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV71445.1"
                     /translation="KLSTPFLLDPAVIGAMGSDSGEAGVLSSMFGAVSLTVGGLPGGA
                     AVSRYLTTQFTIGGMGMDTRQVGVITGTIGVVALVFGGIVVGMIIARFGIKRSLWPMV
                     MAMNVPNLLHVWAAYTHPDQSWVALIVGCDQFGYGFGFAAYMVYLMFITQGVKYPTAH
                     YAISTGLMALGAMGAGIVSGYVQKQVGYAHFFLATLAFATPGMLLLPFLPLEKDDLHT
                     APVDVD"
BASE COUNT          461 a          690 c          567 g          402 t
ORIGIN      
        1 ttctctgcca acagtcggta gcggttttca ctttcgcgca acgtctcgac ggcacgcacc
       61 cgctcagtca catcgttgag cagccagaca tccttgcgca cgccgcggtc ttcccagccg
      121 taggcatgaa actgtaccag tcgacgaact ccgtcagatc ttttaatcca gacttcgcga
      181 ggtcgttgaa aaccatccag gcgatcggac tcccagagtc ggcgaacggt gacggcttca
      241 tcgccaaaca gcagccggaa ccactcggac agcgtgggaa cctgcgctgc cgtgtatccg
      301 gtaatcgcca cgacagcccg gttgatcagg atctcttcgc cactgaccag aactgccccc
      361 gccgggaagt gctcgaccat ggcgcgaaat cgtgcttcgc tctcgcgcag ggcttgaaga
      421 tggtctcgcg attcagacag gttcgccgtg tcgggcgtct ctgcgcgtgg ctccagagag
      481 atcagaagcg tctgaacggc actgcgatac catggttcat ccactggcga gaccgcccaa
      541 cgtcccgcga cggggaacag ccacccacaa ggggtttcaa cctcgaaggc ggcaagttcg
      601 gacaaccggg catccccagg gaaatcctgg acctgcagcc atgctccggg gatacaggtc
      661 ggcagaatct tgacaaaagc ctgcacgacc gcctcaggag gaccaccctc tctcagacgg
      721 cgcaaccagg ggcgaagatc aggcgcttgc atcacaccac ccctcagtcc cgttccatga
      781 actgctgaaa ccaagcacaa tctgacacat gcttcactgt atcgtgtttc tccgccccag
      841 acaaccgttt ttctcttgcc cgagcatcag cgaactggta caaagtcttc atgaattcac
      901 ctgagatcac cccggaaaca cttcttccgc ggctgtacgc atccatcgaa cccttcacct
      961 gtcgccaaag ttgcaccgac tgctgccggg gtatcgtctc tctctcgatt cctgaattac
     1021 agcgcatcga gacatacttc gacgcccgcg gcgaaccact cgccgctcag gcgattgggt
     1081 gcccattccg caccccagac gggtgccggg tgtatccggt tcgtccattc atgtgtcgtc
     1141 tgttcggata ccggtttctg caggcttctc tgcggtcgca gccttcgtgc ccacatctgc
     1201 ctgacgagcg cgacggtgag aaggaacttc aggcgttcac aacgtatcag gcattctgtc
     1261 gcgaacacgg gtttgtcctg atcggtgaaa gcgcagacag gctcagaaga agcgaagccg
     1321 tggccgagaa tcagagcgtc ttacgtgtct ttcccgaact gacccgtttc cgctcctggt
     1381 tgaccggcgg cgatcagctg ctgcccgctc cccggtgatc atcaggatcg cgcaaccgtc
     1441 agcgatcagt cgacgtccac gggtgccgtg tgcagatcgt ctttttcgag cggcagaaag
     1501 ggcagcagca gcataccggg tgtcgcaaac gccaaagtcg ccagaaagaa gtgcgcatac
     1561 ccgacctgtt tctgtacata cccactgacg atgcccgccc ccatggctcc gagagccatc
     1621 aatcccgtcg aaatggcata atgggccgtc gggtatttca ccccctgcgt gatgaacatg
     1681 agatacacca tgtaagccgc gaagccgaac ccgtacccga attgatcaca gccaacgatc
     1741 agggcgaccc atgactggtc cgggtgcgta tacgccgccc acacgtgcag cagattcggc
     1801 acgttcatgg ccatgaccat gggccacaac gagcgtttga tgccgaatcg ggcgatgatc
     1861 atcccgacga cgattcctcc gaacaccagg gccaccacgc cgatcgtacc agttatgaca
     1921 ccgacttgtc tggtatccat gcccatgcca ccaatagtaa actgagtggt cagatatcgg
     1981 gacacggccg ctccacccgg gagccccccg acggtcagac tgacggcacc gaacatactg
     2041 ctgagaacgc cggcctcgcc agagtccgag cccatggctc cgatgaccgc agggtccagg
     2101 agaaacgggg tcgataactt
//
LOCUS       DAORHD010000935         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65547_length_2119_cov_3.607074, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000935 DAORHD010000000
VERSION     DAORHD010000935.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65547_length_2119_cov_3.607074"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PKO06_17210"
     CDS             <1..1408
                     /locus_tag="PKO06_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71446.1"
                     /translation="GEWTQFTPDNSRLPSLGVNRIYRIGGRLAVCTNAGLAIIPLSSQ
                     KYDEFKNRDLGRVSAKTIFPFPDIFKALVPINHLVRKNDFWVASEKGLSRFVSASGLF
                     RSMLNDEAGILSKRPDTSGLREEEQPESVDNRSEGDAPIALSGERVWDFFTKENFLGE
                     ELSYPLPSNKITRLAGDESGHAWMIFEESQLSRLNMVRQPGRPDQRGERVDKPEWYHF
                     TQAAPWSGGVKLAGLWFSEGQLFVGTQSEGYYVLTNPGYSGEKANEWDWKHFGELEGL
                     TRKHVIGFAWRRVPQSEPMLAVLHPDGLSLFDGKDFHAIDLGGGSLRHYRSIVGDPLG
                     NLWMASDAGLIRLTPEWKVLTYTRANANFLSDRITAIAVGSEGKANGISVWAACDQDL
                     NGSDQPPNVVTRQENGRTVKIVQELDIDGASLHYFDGLTWDYWKIPGVRCMYHEGDFL
                     WLGTNWRLRRLSIPALMP"
     gene            complement(1595..>2119)
                     /locus_tag="PKO06_17215"
     CDS             complement(1595..>2119)
                     /locus_tag="PKO06_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNV71447.1"
                     /translation="NKSLRGGLETLEGKLANLTDYFDCRPISINRSFRWETLEFTPVQ
                     TVHVVSGMKIQHSYGLLIREDGSERAVFFSTDTQFAPYQMRKILERADLIFHDCETAP
                     FKSGVHAHYDDLKTLPSALKSKIWLYHYQPRPTPNATEDGFLGFVTKGQEFIIEADRR
                     IQPFPPDEGVAKAA"
BASE COUNT          446 a          625 c          607 g          441 t
ORIGIN      
        1 cggcgaatgg acccagttca cccccgacaa cagccgcctg ccgtccctgg gcgtcaaccg
       61 catctaccgc atcggcggcc gcctggctgt ttgtacaaat gccggtcttg ccatcatccc
      121 gctcagttct cagaagtatg atgaattcaa gaatcgcgat ctcggtcgcg tgtcggcgaa
      181 aaccatcttt ccctttcccg acatcttcaa agcgttagta cccatcaatc atctggtacg
      241 gaagaacgat ttctgggtgg cgtccgagaa ggggctgtcc aggttcgtca gcgcttccgg
      301 tctgttccgt tccatgttga acgacgaagc gggcattctc tcgaaacgac ctgacaccag
      361 cggtctgcgc gaagaagaac aaccggagtc ggtggacaac cgcagcgaag gtgacgctcc
      421 catcgctctc agcggcgaac gcgtctggga ctttttcacc aaggagaact tccttggtga
      481 agaactctcc taccctcttc cctcgaacaa gatcacccgg ctggccggtg acgaatccgg
      541 gcatgcctgg atgatcttcg aagaaagtca gttgtcacgc ctcaacatgg tcaggcaacc
      601 cggacgcccg gaccagcgcg gcgagagagt cgacaagccg gagtggtatc acttcacaca
      661 ggccgctccc tggtccgggg gcgtgaaact cgccgggctg tggttttcgg aagggcagtt
      721 gttcgtgggc acgcagtctg aaggatacta tgtgctgacc aaccccggat actcgggcga
      781 aaaggcgaac gagtgggact ggaagcactt cggagagctc gagggcctga cccgtaagca
      841 tgtcatcgga tttgcctggc gccgtgttcc gcaatctgag ccgatgctgg ccgttctcca
      901 cccggacgga ctttctctct tcgatggcaa ggactttcat gcaatcgatc tgggcggcgg
      961 ttcactgcgg cattatcgtt ccatcgtcgg cgatccgctc ggcaatctct ggatggcttc
     1021 agacgccggt ttgatcagac tgactccaga atggaaggtg ctcacctata ccagggcgaa
     1081 cgccaatttc ttgagcgacc gcatcaccgc aattgctgtg ggctctgaag gaaaagccaa
     1141 cggaatctcc gtgtgggcgg catgcgatca ggatctgaac ggttcagacc aaccgccgaa
     1201 tgtcgtcacc agacaggaga acggaagaac cgtgaagatc gtccaggaac tggacatcga
     1261 cggcgcatct ctgcactact tcgacgggct gacgtgggac tactggaaga ttcccggcgt
     1321 gcggtgcatg tatcacgagg gcgactttct ctggcttggc acgaattggc gcctgcgccg
     1381 cctctctatt ccggccctca tgccctgacc gactcggaca atccctcgcg ccgcacctcg
     1441 ggcaagcccg cacccatggg tctctgagcg atcttgactc gcgaacagct gatctcacct
     1501 aggccctgga cttcgccaca ctcttcgcgc aaagacactc ggggaccaga ttttcatctg
     1561 acccccgagg aagaaacacg tcgacggagg aaccctaggc ggccttggcg accccctcgt
     1621 caggcgggaa cggctgaatg cgacggtcgg cttcgatgat gaactcctgg cctttggtca
     1681 caaaacccag aaatccgtct tccgtcgcgt tcggcgtcgg tcgtggctga tagtgataca
     1741 gccagatctt gctcttgaga gcggagggca gagtcttgag gtcgtcgtag tgggcatgaa
     1801 cgcccgactt gaagggagcc gtctcgcagt cgtggaaaat cagatcggct cgttcgagga
     1861 tttttcgcat ttgatacggg gcaaactgag tgtccgttga gaaaaacacc gcacgttctg
     1921 atccgtcttc gcgaatcagc agaccgtaac tatgctgaat cttcatgccg ctgacgacat
     1981 gcaccgtctg caccggcgtg aactcgagcg tttcccaccg gaacgagcga ttgatcgaaa
     2041 tcggacgaca gtcgaagtag tcggtgagat tcgccagttt accttcgagt gtttcgagac
     2101 cgccgcgcag gctcttgtt
//
LOCUS       DAORHD010000936         2113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65844_length_2113_cov_4.186103, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000936 DAORHD010000000
VERSION     DAORHD010000936.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65844_length_2113_cov_4.186103"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PKO06_17220"
     CDS             <1..588
                     /locus_tag="PKO06_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71448.1"
                     /translation="PIGIEQIESQLTTHPSGLKVLLSPPVPENAERIRPELMEHIVET
                     LRGHYEYVVIDTQNQLTELELKFLEMSDQILLMMTMELTFIKNTKLLLDLFQRLKIPR
                     DKVKVVLNRAFKTMGLEPTRVEASLRYAISHFIPSDGEIVIPSVNRGEPFVMQNLEGS
                     PLMMAMEKLCQRMVGEEPDKGTWSMFSMIREVLGI"
     gene            complement(711..1469)
                     /locus_tag="PKO06_17225"
     CDS             complement(711..1469)
                     /locus_tag="PKO06_17225"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNV71449.1"
                     /translation="MEYKAFAVTNVGKVRTNNEDTYTVTERDGFFLVADGMGGHKAGE
                     VAAAMTRDIIAEKLSQTKGATDLENQIRSAFQAANEQVHSKARKGTGCEGMGCTCVSL
                     MLREQNFYMAHVGDSRAYLLRKGELKQMTRDHSYVEELFIRGLISEEEKVDHPYKHQI
                     TRYVGCTSKIEVDITSGPVWNGDLFMLCSDGLSEMVPFNRIQEICSAAQEANEIAEAL
                     VQEALNNGGKDNVTVVVVQVTSKKTSFFRKILGW"
     gene            complement(1484..>2113)
                     /locus_tag="PKO06_17230"
     CDS             complement(1484..>2113)
                     /locus_tag="PKO06_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71450.1"
                     /translation="LIGTSPVQLTVKQGDYKIVCTLPGYRDNAMRLNLAGKNTMVVRR
                     RLNRLKPELLDTLNDLKRSLPPYPTGAKPKDKALERFHVEWKRIEELLDENPIDEDLH
                     DRFVELTKSRGLLNFAETYYRSRWEKEKEYPLLLTMLGRVQFLKNDMQKSLDTLSESW
                     FIDPNSIPLLNYLGEYFLKEGDKVKAKQYFQLSVSLRKDPEIESKILDL"
BASE COUNT          454 a          596 c          548 g          515 t
ORIGIN      
        1 cccatcggca tcgaacagat cgagtcacag ctcacgaccc atccgtccgg gctgaaggta
       61 ctgctctcgc cgccggttcc cgaaaatgcc gaacgcatac ggccggaact tatggaacac
      121 atcgtcgaaa cgctgcgtgg ccattacgag tatgtcgtga tcgatacgca gaaccagctg
      181 accgagctcg aactcaagtt tctggaaatg agcgatcaga ttctgctgat gatgaccatg
      241 gaactcacgt tcatcaagaa taccaaactg ctgcttgacc tgtttcagcg gttgaagatc
      301 ccgcgcgaca aggtcaaggt cgtgctcaat cgcgcattca agaccatggg tctggaaccg
      361 acacgcgtcg aagcctcgct gcgctatgcc atcagtcact tcattcccag tgacggggag
      421 atcgtcattc cctcggtcaa ccgcggcgag ccttttgtca tgcagaacct cgaaggctca
      481 cccctgatga tggccatgga aaagctgtgc caacggatgg ttggcgaaga acccgacaaa
      541 ggcacctgga gcatgttttc gatgatccgc gaagtgctcg ggatctagcg gaagccgaga
      601 ctctcgcagc gccggaacaa gaacactccc cgctcattct agtgagcggg gagtgttttt
      661 gtcctggttc tttctgacga tttactgcat cattcgccag ttacaccgtc ttaccaacca
      721 aggatcttgc gaaagaagct ggtttttttc gaggtcacct gaacaacgac gaccgtgacg
      781 ttgtccttgc cgccattgtt cagcgcttcc tgtaccagcg cttcggcgat ttcattggct
      841 tcctgagccg cactacagat ttcctgaatg cgattgaacg gcaccatttc gctgagtccg
      901 tccgagcaca gcatgaacaa atcgccgttc cagacggggc ctgaggtgat atcgacttcg
      961 atcttgctgg tacacccgac gtatcgggta atctgatgtt tgtaggggtg atcgaccttc
     1021 tcttcttcgg agatgagacc acgaatgaac agttcttcga cgtaggaatg atcccgggtc
     1081 atctgcttca actcgccctt gcgcagcagg taggcgcggc tgtcgccgac atgagccata
     1141 tagaagttct gttcacgcag catgagcgag acacaggtac accccatgcc ttcgcaaccg
     1201 gttcccttgc gggccttgct gtgcacctgc tcgttggcag cctggaatgc tgatcgaatc
     1261 tggttctcca gatcggtcgc gcctttcgtc tgcgacagtt tttcggcgat gatatcgcgc
     1321 gtcatcgcgg cggccacttc tcccgccttg tgaccaccca tgccatccgc aacgaggaag
     1381 aacccatcac gttcggtgac cgtatacgtg tcctcgttgt tggtgcgcac tttcccaaca
     1441 tttgtgactg caaaggcttt gtattccacg tgagttcccc ggtctacaaa tctagaatct
     1501 tgctttcgat ctcagggtct tttcgcaagg acacagacaa ttggaagtac tgtttggctt
     1561 tgaccttgtc gccttctttc aggaagtact cgcccaggta attcagcagc ggaatcgagt
     1621 tcggatcaat gaaccacgac tccgacaagg tatcgagcga tttctgcata tcattcttga
     1681 gaaactgcac tcgacccagc atggtgagca gcagcggata ctccttttcc ttttcccagc
     1741 gcgaccggta ataggtttcg gcgaagttca acagaccgcg agacttggtc aactccacga
     1801 atcgatcgtg cagatcttca tcaatcggat tctcgtccag gagttcttcg attcgcttcc
     1861 attcgacgtg aaacctttcg agggccttgt ccttgggctt cgcgccggtg ggatacggcg
     1921 gcagactgcg tttgaggtcg ttcaaggtgt cgagcagttc cggtttcagc cgattcaacc
     1981 gacgtcgcac gaccatggtg tttttcccgg ccaggttgag acgcatggcg ttgtcacgat
     2041 atcccgggag agtacagacg atcttgtagt caccctgttt gacggtcaac tgtaccgggc
     2101 tggtaccaat caa
//
LOCUS       DAORHD010000937         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65918_length_2112_cov_2.834711, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000937 DAORHD010000000
VERSION     DAORHD010000937.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65918_length_2112_cov_2.834711"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..2087
                     /locus_tag="PKO06_17235"
     CDS             123..2087
                     /locus_tag="PKO06_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71451.1"
                     /translation="MMTKAASKNWRNWRMLLGGFALLALFTLYGCTTGVKKDPGLTDF
                     EAPAIPTNVVAVGKEKAVFLTWSANTEADLVGYRVYRSTSSGGPFTLIATVGKMAAPS
                     ILDNDNQNGLVNDQYYYYKLSAFDTQGKESDLSRTNAIQARAGLPVEERPPRVVNIKA
                     RASTEEVYISWDKVTGISIKGYNIYRGLSTSAGGVQWIAAVPQETPGFVDKSVSKSSA
                     EQYTYVIRTYNEMYTESENSDPVQVTLRSGDDTIPKPPYDVAVSNDTDPIVSWHKPIQ
                     NEDGSNIFDGNNPTLDLDSYLIFRANQTDRLFSLIGIIEDNGTANLTQTFKDLNGSPY
                     NLYAVRAIDRNGNVSGQSSIVTQSSDVDIPAIPTGLTAYSSTLTESGIKLAWNAAKNA
                     NSYNVYFSTVADGGYTKMLTGQPQWTQTSNYVITNYPSNFNQLPEKKGQKLEFGVPYF
                     FKVSAVSASGKESDLSSYAKAYPGGVWVGMLEGEDWNWGFNDRNAPASPIAGRIPIRN
                     SLNYPNIDYFSGAGAVLLVADTVGTTGNGDAMEWGFGNSSANYYNLPTPTSGYYRFNL
                     YAYVYPHTTGGTWRLKVSENGLIFTTTLMEKDINGYSSINKGRSVIPLGQILVQSTVD
                     GVDLELTCVNPGAGGGTSLLLDSIAFVQVQ"
BASE COUNT          489 a          615 c          568 g          440 t
ORIGIN      
        1 caggcgattc gcgaagccga gttcctgagg tgtgtgcatg cgcgctaatc aaattctgat
       61 gacgtgacga taatgagaga tgagaaaaac catttcccca ttggggaacg aaggagtgcc
      121 ttatgatgac caaggccgca tctaaaaact ggcggaactg gcggatgctg ctgggtgggt
      181 tcgccctgct tgcactcttc actctctacg gctgtacgac cggggtgaaa aaggatcccg
      241 gcttgacaga ctttgaagca ccggcgattc cgaccaatgt cgtggccgtc ggcaaagaga
      301 aagccgtgtt tctgacctgg agcgccaaca ccgaagcaga tctggtcggg tatcgtgtct
      361 atcgctcgac cagttcgggc ggaccgttca ccctgattgc cactgtcggc aagatggcag
      421 cgcccagcat tctcgacaac gataatcaga atggcttggt gaacgatcag tactactact
      481 acaagctgtc cgccttcgat acgcagggca aagagtctga cctgtcgcga acgaatgcga
      541 ttcaggctcg cgccggtctg cccgtcgaag aacgaccgcc ccgcgtcgtg aacatcaagg
      601 cccgtgcttc gaccgaagaa gtctacatct cctgggacaa agtcaccggc atcagcatca
      661 agggatacaa catctatcgc ggtctctcga ccagcgctgg tggcgtgcag tggatcgcgg
      721 ccgtgcctca ggaaacgccc ggatttgtcg acaaatcggt gagcaagagt tccgccgagc
      781 agtataccta cgtgattcgc acctacaacg agatgtatac cgagagcgaa aactctgatc
      841 cggtgcaggt aacattgcgc tccggcgacg acacgattcc gaaaccaccc tacgatgttg
      901 cggtcagcaa cgacaccgac ccgatcgtct cctggcacaa accgattcag aacgaagatg
      961 gctcgaacat tttcgatggg aacaacccga cgctggatct cgattcctac ctgatcttcc
     1021 gggcaaatca gaccgaccgc ctgttctccc tgatcggtat catcgaggat aacggtactg
     1081 ccaatctgac ccagaccttc aaagacctca acggctcacc gtacaacttg tacgccgtgc
     1141 gcgccattga tcgcaacggc aatgtcagcg gacagagctc gattgtgacc cagtcttccg
     1201 atgtcgatat tcctgccatt cccaccggtt tgacggcgta ttcctcgact cttacggagt
     1261 ccggtatcaa gctggcctgg aacgcggcaa agaatgccaa ctcgtacaat gtctacttct
     1321 caacggtcgc cgatggcggc tacaccaaga tgctgaccgg tcagccgcag tggacgcaga
     1381 ccagcaacta cgtcatcacc aactatccga gcaacttcaa ccaactgcct gagaagaaag
     1441 gccagaagct ggaattcggc gtgccgtact tcttcaaggt gtcggcggtg agcgcttcgg
     1501 gcaaagagag cgatctcagt tcctatgcca aagcctatcc gggcggcgtc tgggttggta
     1561 tgctcgaagg tgaagattgg aactggggat tcaacgatcg caatgctccg gccagtccga
     1621 tcgcgggtcg tatcccgatt cgcaattcgc tgaactatcc gaacattgat tacttctctg
     1681 gcgctggagc cgtgctgctc gtcgctgata ccgtcggaac cacgggcaac ggggatgcga
     1741 tggaatgggg tttcggtaac tcttcggcca actactacaa cctgccgacc ccgacctccg
     1801 gctactatcg gttcaatctg tatgcctatg tgtatccgca taccaccggt ggcacgtggc
     1861 gtctgaaggt ttccgaaaac gggttgatct ttacgaccac cttgatggaa aaagacatca
     1921 atggctactc ttcgatcaac aaaggtcgct ccgtcattcc gctgggccag attctggtac
     1981 agtcgaccgt cgacggggtc gatctcgaac tcacctgcgt gaacccgggg gccggcggcg
     2041 gcacttcgct gctgctcgac tcgatcgcct tcgttcaagt gcaataacga tctcgcggat
     2101 caaccaccct cg
//
LOCUS       DAORHD010000938         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_65919_length_2112_cov_2.766650, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000938 DAORHD010000000
VERSION     DAORHD010000938.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65919_length_2112_cov_2.766650"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKO06_17240"
     CDS             complement(<1..277)
                     /locus_tag="PKO06_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71452.1"
                     /translation="MTHSPTLLMEVPFLLVIVCMGTTVLASLGCGLIKYVATKRSVLL
                     DVPNERSSHSQPMPRGGGGPMAVAALLAIALIGLVRPELLAKPWIMAL"
     gene            complement(356..1360)
                     /locus_tag="PKO06_17245"
     CDS             complement(356..1360)
                     /locus_tag="PKO06_17245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV71453.1"
                     /translation="MKILITGASGFVGTELLRFWRSSGHEITGTSHVTPFPMADCTRV
                     SVDPRQRHVETGQKESSISWLLVDDLVSTDWDKIVVGHDVVVHLAARVHQMNDGPDAA
                     RLYFQTNTEGTRRLAESAQKAGVKRFVFISTVKAMGEETSDGATWTEDTPCQPLDAYG
                     ESKRAAEQLLLEMHRQSGFPAVILRPPLMYGPGVKGNMASLMHLVESGWPVPVGGIAN
                     RRSFLSVSNLASAIDAVIDHAAAVGQVFLVTDGIPMSTAQLVAVMGDAIGRKPWIIGF
                     PEIIWRLGAKLPFLGSRVRRLTGSLALSSVRISTEIGWKPPFSMLDTLRETFSARQKN
                     "
     gene            complement(1380..>2112)
                     /locus_tag="PKO06_17250"
     CDS             complement(1380..>2112)
                     /locus_tag="PKO06_17250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV71454.1"
                     /translation="HLERLLPTLMPAAERCGATILMVDNRSRDGSSDFVRTQYPRVQV
                     VSNPLRTGYGGNHNINMRRADSDYFVIMNSDMTVQVDTFEKLAQFMDANPDIGICAPK
                     IVNEDGTIQGLNKRIPTVFDLFARKFLPPWIQRFFQRRLDAYEMRDIGYDHQYDSPLL
                     SGAFMFCRTDLLKRLGGFDESYFLYFEDYDLCRRVQKTHRTVYVPVTEVVHFWERSAH
                     KSHKYALWFMEGAARFFWRWGVKLA"
BASE COUNT          460 a          640 c          577 g          435 t
ORIGIN      
        1 acagagccat gatccagggt ttggcaagca attcgggccg caccagcccg atcaacgcga
       61 tcgccagcag cgccgccacc gccatcggcc cgcccccacc ccgcggcatc ggctgcgaat
      121 gcgaactccg ctcattcggc acatcgagca acaccgaccg cttggtcgcc acatatttaa
      181 tcagcccgca cccgaggctg gccagcaccg tcgtgcccat gcagacgatg accagaagga
      241 acggaacttc catgagcagg gtcggagaat gcgtcatggt ctcagtcttt cagtgtcagg
      301 agtcgtgagt gtgaacgaca tgccagtcga gtgtaatcga aaaacgggcg atcagctagt
      361 ttttctgtct cgcggagaac gtctcacgca gcgtgtcgag catcgagaag ggtggcttcc
      421 agccgatttc tgtgctgatt cgaacgctgc tcaaggccaa cgaccctgtc agccgtcgca
      481 cgcgtgaacc gagaaacggc agcttcgctc cgaggcgcca gatgatttcc ggaaatccga
      541 tgatccaagg tttgcgaccg atggcgtcac ccatcacggc aaccaattgc gccgtcgaca
      601 tgggtattcc gtccgtgaca aggaagacct gaccaaccgc agcagcatgg tcgatgacgg
      661 cgtcgatggc gctcgccaga ttgctcacgc ttaagaaact gcgtcgattc gcaatcccac
      721 cgactggtac gggccaaccg ctctcgacca gatgcatcag gcttgccatg ttgcccttca
      781 ctccgggtcc atacatcagg ggcgggcgca gaatgacggc cgggaaaccg ctctgccgat
      841 gcatttccag caggagttgt tccgcggcgc gtttcgactc tccgtaggca tcgagcggct
      901 gacagggggt atcttccgtc caggttgctc catccgacgt ttcttctccc atggctttta
      961 ccgtgctgat gaaaacaaat cgcttcaccc cggctttttg tgcactttcg gccagacgcc
     1021 gagtgccctc ggtgttagtc tggaagtaca gcctggccgc atcaggcccg tcattcatct
     1081 gatgcacccg cgcggcaaga tgaaccacga catcgtgtcc aaccacgatt ttgtcccagt
     1141 cggtggagac gagatcgtcg accagaagcc atgatattga actctctttc tggccagtct
     1201 cgacatgtcg ctgtcgcggg tcgacggata cccgcgtgca gtcagccatc gggaaaggtg
     1261 tcacatgcga cgttcccgtg atctcgtgtc ctgaagatcg ccagaatctg agcaactctg
     1321 tgccgacgaa gcccgacgcg cccgtgatca gaatcttcat ggagttgcta cctcggacct
     1381 catgccaact tcacgcccca tcgccaaaag aaacgtgccg cgccttccat gaaccagagt
     1441 gcatacttgt gactcttgtg cgccgagcgc tcccagaagt gtaccacctc ggtgaccggc
     1501 acatagacgg tgcgatgcgt tttctgcacc cggcgacaga gatcgtagtc ttcgaaatag
     1561 agaaagtacg attcatcaaa cccgcccagc ctctttaaca gatcagttcg gcagaacatg
     1621 aaggctccgc tcagcagagg cgaatcatac tgatggtcgt aaccgatgtc gcgcatctcg
     1681 tatgcatcga gacgccgctg aaagaaacgt tgaatccagg ggggaaggaa cttgcgcgcg
     1741 aacaagtcga acaccgtcgg aatgcgcttg ttgagccctt gaatggttcc gtcctcattc
     1801 acgattttcg gagcgcagat gccgatatcc ggattcgcat ccatgaactg cgccagcttt
     1861 tcgaaggtat caacctgcac ggtcatgtcc gagttcatga tgacgaaata gtccgagtcg
     1921 gcacgacgca tattgatgtt gtgattgccg ccatagccgg tacgaagtgg gttgctcacc
     1981 acctgcaccc tcggatactg cgttctcacg aagtcactgc ttccgtcgcg tgagcggtta
     2041 tcgaccatga gaatcgtggc gccgcatcgt tccgccgccg gcatcagagt cggcaggagt
     2101 cgttccagat ga
//
LOCUS       DAORHD010000939         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66022_length_2110_cov_2.976642, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000939 DAORHD010000000
VERSION     DAORHD010000939.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66022_length_2110_cov_2.976642"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..495
                     /locus_tag="PKO06_17255"
     CDS             199..495
                     /locus_tag="PKO06_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71455.1"
                     /translation="MKLLVQLIVMLGMIGLLVQMPISVVLHRFSSGTVFPGLEAPVHA
                     FPLMVRVAKGSSPLWYRAANVFLLGIDLLVLLGLAAYPAHLLGRRGAGDHRPDR"
     gene            complement(553..1920)
                     /locus_tag="PKO06_17260"
     CDS             complement(553..1920)
                     /locus_tag="PKO06_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71456.1"
                     /translation="MTHALPTPVAQPRSSSPNHTASPAQLQPVSELSSSVSMLADNLP
                     SEEMELEEDELANAEAGEESTASPSIHLLTPRFHWIPALGVEIRSGTESRFSPASTSQ
                     DIAESAHIRFYRRSSLGTTATPAAILLPPNGTPLSFHPGAVFQLSSRRLSVRLGRYHL
                     VLPRVSDFEILFPSGAIFHLNAGEVFFELDAQGEGTIAIPRGSGWLKDVARKTQKLGA
                     GKQITIAATGVLARPREIDSRWKSRTYQGFAPVPERATNKPTSEVPEEVQEALADLEN
                     TDDSSSSSPDNPREDPLQLASSTRDPGASFPASISSEAAVLATQSAHLPDKPKTTDQL
                     PQVSNSLMSYMSPEARALLLANPAAANPRLASESAPSPVRGAPTQDLLQQLTRQVPEL
                     KDLAVPGSSPSSKANDLFRQLQGAAIPIPPITWKYLVFPGFPPIPIPSMALPTGLLPI
                     PKLRR"
BASE COUNT          444 a          545 c          680 g          441 t
ORIGIN      
        1 gcaggtaagg atccagaaca gggtcatgga tgcctccttc gttagctgac agtgtacttc
       61 tctcagttat cggccgaact cccgcaatcg tgtagccaga tactctttca tcacattcag
      121 tggcacggca cattgaagcg cggtatacat gtctgagata aagccagttt ctgaagggga
      181 gccgggtgtc ggcgaactat gaaactcctc gtgcaattga tcgtgatgct ggggatgatc
      241 ggccttctgg tccagatgcc gatcagtgtc gttctgcacc gcttcagcag tggcactgta
      301 ttcccgggtc tggaagctcc ggtgcacgca tttccgctga tggtgcgggt ggcgaaagga
      361 agctctccgc tctggtaccg agcggccaat gtgtttctgc tcggcatcga cctgctggtg
      421 ctgctcggcc tggctgcgta tccggctcac ctgctgggtc gacggggggc gggcgatcac
      481 cgccccgatc gataacgaaa gcgaatgcgc ggtggcattc tccgggcacc cccgatcatc
      541 aagaaccgtg agttacctgc gcagtttggg gatgggcagg agacctgtgg ggagagccat
      601 tgagggaatc gggatcggtg ggaaaccggg gaaaaccaga tatttccagg tgatgggggg
      661 gatgggaatg gctgcaccct gcagctgtcg gaacagatca ttggctttcg acgatggtga
      721 tgatccgggg acagccaggt ctttcagttc cggaacctga cgagtcagct gttgcagcag
      781 atcttgtgtg ggtgctcccc ggacaggact cggtgccgat tccgaggcta ggcgcgggtt
      841 cgctgccgcc ggatttgcca acagcagagc gcgggcctcg gggctcatat acgacatcag
      901 agagtttgag acctgcggca actgatcggt ggtcttgggc ttgtcgggca ggtgcgccga
      961 ctgggtcgcc aggacagccg cttcggagga aatggatgcc gggaatgaag cccccggatc
     1021 tcgagtggac gaagcgagct gcagaggatc ttcgcgtggg ttatccggag agcttgagga
     1081 agaatcgtcc gtattctcca gatccgcgag tgcctcctga acctcttcag ggacttctga
     1141 agtcggtttg ttggtggctc gttcgggtac tggcgcaaaa ccttgatagg ttcggctttt
     1201 ccaccgcgag tcgatttcac gtgggcgtgc cagaactccc gtcgcggcga tggtaatctg
     1261 ttttcccgcc ccgagtttct gggtcttgcg agcgacatct ttcagccaac ccgatccgcg
     1321 tggaatggca attgtgcctt cgccttgagc gtccagttcg aaaaaaacct caccagcgtt
     1381 cagatgaaag attgcgccag atggaaacaa aatctcgaag tcactgacgc gaggcagaac
     1441 gagatgatac cgaccgagtc gaacagacag acggcgcgac gaaagttgaa aaacggcacc
     1501 gggatgaaag gacaacggtg tgccgttggg cgggagcaga atcgcggccg gagtggcggt
     1561 cgttccaagt gatgatcgtc gataaaaccg aatgtgagct gattcagcaa tatcctgact
     1621 tgtactcgcg ggggaaaacc gggactccgt accggaacgg atctcgacgc ccagcgccgg
     1681 aatccagtgg aatcgggggg tgagcaggtg gatggacgga gaggccgttg attcttctcc
     1741 ggcttccgcg ttggccaatt catcctcctc gagttccatc tcctctgatg gcaggttgtc
     1801 agcgagcatg gaaacggaag aagaaagctc agatacaggc tggagttgtg ccggtgaggc
     1861 ggtatggttg ggcgatgacg accgtggctg ggcaaccggt gtcggcagag cgtgcgtcac
     1921 caccactgtc cagaaaaaaa ggacgattcc tgacaacagg ccacgacaga aagacgggcg
     1981 agggagcagg ccgtgggttt gacgggaaat tccgaacctc gaatcagtcg tccttctgat
     2041 caatgtcatc ggcagtacca gactcggatg tcggcaggga ctccccgttc ttcgctgact
     2101 tctccacctg
//
LOCUS       DAORHD010000940         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66317_length_2105_cov_3.152683, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000940 DAORHD010000000
VERSION     DAORHD010000940.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66317_length_2105_cov_3.152683"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="PKO06_17265"
     CDS             <1..1922
                     /locus_tag="PKO06_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNV71457.1"
                     /translation="SKEIYYRPDSTDADELRVCTGAWIDDQTLDAGGFPGRNRLRRMT
                     LQATTQALAGFSRVSIEARRIHVYLGCMDPDESYATWRFSRREIPRIRERLQHDCHVD
                     ESAWETAIRAYVTANHVSSEEYQAAMLTSSVLHQMREVFGVRGEVGLVDAACASSLAA
                     IDHAIHVLHTGTADLVISGGIEANLGPESFALFSVLGALAADHCLPFDRHGTGLSLGE
                     GAVILALERLEDALRLNHPIHGILKACGASSDGRSSSLFAPTIAGQKRAYAQAYAGLD
                     LDDLVYLECHGTGTRIGDETELRSVNEFFRRPQLPIGSAKALIGHTKGAAGAVGVLKC
                     LLSLQHRVIPPSRYFREPCLTSLNCMINTTPLTIHNSDKPALMGISSFGFGGINYHLV
                     IEEYRAHGGVKPTEYPASHRVYISVDRFFPWDELAQLSHLDHLHVPPNSRPQIDRAQL
                     GALNAVAEITRELRLDWSAIPAERIQVISASSLGLDACVDLTTRLRYAQVRTIFPQLP
                     EAALNGILELRQRFSRITSDSSPGVLNNVLAGRVANAFDFHGPNFNVDADNNGLSLAL
                     SIASRRLMTCPGVVIILALREEISSDGAAIDRSGVHCLILSSEEHAWKCGLPLREMVE
                     KVDYDPAVGGVGNHA"
     gene            1915..>2105
                     /locus_tag="PKO06_17270"
     CDS             1915..>2105
                     /locus_tag="PKO06_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71458.1"
                     /translation="MRETRDVNGNVRWDSQGAFGDGYVITRVENPGCDPGRLAIMFSG
                     QGSAKPGMFLDLYQRVQVVR"
BASE COUNT          409 a          573 c          616 g          507 t
ORIGIN      
        1 ggtctaaaga gatttactat cgaccggatt ccaccgacgc ggatgaactc cgtgtctgca
       61 caggagcttg gattgacgat cagacacttg atgccggcgg attcccgggt cgaaatcgct
      121 tgaggcgcat gacgttgcag gcgacgactc aagctctggc cggattctcg cgtgtctcaa
      181 ttgaggctcg tcggatacac gtgtatctcg gttgcatgga tcctgatgaa tcgtacgcaa
      241 cctggcgttt ttcgcggcgc gagattcctc gcattcgcga acgactgcag cacgattgtc
      301 acgtcgacga gtcggcttgg gaaacagcca ttcgagccta tgtgacggca aatcacgtca
      361 gttctgagga atatcaggct gccatgctga catcatctgt gttgcaccag atgcgtgagg
      421 tttttggtgt tcgcggcgag gttggtctgg ttgatgccgc gtgcgcctct tcgctggctg
      481 caatcgacca tgccatccac gtgttgcata ccggaacagc ggatcttgtc atcagtgggg
      541 gcatcgaggc gaacctcggt cccgaatcat ttgccctctt ttcggttctg ggagctcttg
      601 ccgctgacca ttgtctgccc tttgatcggc atggtactgg cctgagtctc ggtgaagggg
      661 cggtaatcct tgcgcttgaa cgtctcgaag atgcgcttcg tctcaaccat ccgattcacg
      721 ggatactgaa agcctgcggg gcatcgagcg acgggcgatc gtccagtctc tttgccccga
      781 ccattgcagg ccagaagcgc gcgtatgccc aggcatacgc cggtcttgat ctcgatgatc
      841 tcgtgtatct cgaatgtcat ggaacaggta cacgaatcgg cgatgaaact gagttacgtt
      901 ccgtgaacga gttttttagg cggccgcaat tgccgattgg atcagcaaaa gccttgattg
      961 gtcataccaa gggcgctgca ggtgccgtgg gtgtgttgaa gtgtctgctg agtttgcagc
     1021 accgtgtgat tccaccgtcc cgctatttcc gtgaaccgtg tctcacgagc ctgaattgca
     1081 tgattaacac gacacccctg actattcaca actcggacaa acctgccctg atgggaatct
     1141 cgagttttgg gttcggtggc atcaactatc atctcgtgat cgaagaatac cgtgcacatg
     1201 gcggcgtgaa gccgacggaa tatccagcct ctcatcgcgt atatatcagc gttgatcgtt
     1261 tctttccctg ggacgaactg gctcagcttt cgcatctgga tcacttgcat gtgccgccga
     1321 acagtcggcc gcagatcgac cgggcgcaac tcggggcgct gaacgcggtg gccgagatta
     1381 cacgcgaact ccgactcgat tggagcgcga ttccggctga gcggatccag gtgatttctg
     1441 cgagttcgct tggactcgat gcctgtgtcg acctgaccac gcgcctgcga tacgcccagg
     1501 ttcgcaccat ttttccgcaa ctccccgagg cggcgttgaa cggcatcctt gagctgcgcc
     1561 agaggttttc ccgcatcacc tcggactcaa gccccggcgt gctgaacaac gttctcgctg
     1621 gacgtgtcgc caatgctttt gactttcatg gccctaactt caatgttgat gctgataaca
     1681 atggcctgtc actggcactg agcattgctt cgcggcgact gatgacgtgt ccgggagtcg
     1741 tcatcattct ggcgctccgt gaagagattt ccagcgatgg ggcagcgatt gaccgctcag
     1801 gcgttcactg cctcatcctg tcttctgaag aacacgcgtg gaaatgtggt ctgcctttgc
     1861 gcgagatggt cgagaaggtt gactacgatc ctgccgttgg cggagtcggg aaccatgcgt
     1921 gagactcgag acgtgaacgg caatgtcagg tgggactcgc aaggtgcctt tggcgacgga
     1981 tacgtgatca cgcgggtcga aaaccccggc tgcgatccag gaagactggc gatcatgttt
     2041 tccggccagg gctcggcgaa gccgggaatg tttcttgatc tgtaccaacg ggttcaggtg
     2101 gttcg
//
LOCUS       DAORHD010000941         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66714_length_2097_cov_3.410872, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000941 DAORHD010000000
VERSION     DAORHD010000941.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66714_length_2097_cov_3.410872"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2097
                     /locus_tag="PKO06_17275"
     CDS             <1..>2097
                     /locus_tag="PKO06_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71459.1"
                     /translation="DLTLSGFLSFLKKHYTFIDDLLVFNSDGVLISASNVGLLSHGDA
                     WVASASAWYQAELQRRSVSSTSVDVSCRVEPRTCNAVIVDIVAWIYENDRKKDLIGAM
                     VLKSPFQIESHCDPNTFSIVLDDTGRPVSYPDFVSTDSLSLIVEACASSAQPVLQSRI
                     ADTEYLIGVARTEQISHHFPSRLTVLALRSTERAFQPLISPLRSSLLYMVLLGLLGLL
                     ALLIFALENSRFYQQLQETTENLQNLIASTPSPIISWNRDSSITLINPAFEQFSGWSA
                     SELIGQRPEFIFAIDDAFLDACQRANCGNNWKSLELAIRHKNGNLLFGLWNSANLYGP
                     EKTIVATIVQGIDITERKKAENVLLETNRQLEQATAQAQAASIAKSEFLANMSHEIRT
                     PMNGVIGMTGLLLETNLSVEQRSYAETVRSSAESLLSLLNDILDFSKIEARRLTLEIL
                     PFDLSSLLDDLADSLAIRAHDKGVELICAIDPAVPDLVQGDPGRLRQIINNLAGNAIK
                     FTRKGEVILRVTVAEDQRDYVILRFSISDTGIGIPREKQSLLFEKFSQVDTSVTRKYG
                     GTGLGLAISKQLSELFNGNIGFNSEEGKGSEFWFTARLQKMQDGAIASIEKTATLQGV
                     SVLIVDDNQTNVDFLSARLQSWGMKTTAVLTGTDALEQLRRAHEAGTPFPLAILHMQM
                     PGMDGKTLGRAILADP"
BASE COUNT          453 a          642 c          546 g          456 t
ORIGIN      
        1 ctgatctgac gctttccggc ttcctgagct tcctgaagaa gcactacacg ttcatcgatg
       61 acctgctggt cttcaactct gatggggtcc tgatttccgc cagtaacgtg gggctgctgt
      121 ctcacggcga tgcctgggtc gcttcggcat cggcctggta ccaggccgag cttcagcgtc
      181 gctctgtctc ctcgacctcg gttgacgtgt cctgccgtgt tgagccccga acctgcaatg
      241 ccgtcatcgt cgacattgtc gcgtggatct atgaaaacga ccgcaagaaa gacctgatcg
      301 gagccatggt gctgaaatcc ccgtttcaga tcgaatcgca ctgcgatccg aataccttct
      361 ccatcgtcct ggacgacacc ggacgaccgg tttcctaccc ggacttcgtc tcgaccgaca
      421 gtctttcgtt gatcgtcgaa gcctgcgcct catccgccca gccggttctg cagagccgca
      481 tcgccgacac cgagtatctg atcggcgtag cccgcaccga gcagatctct catcactttc
      541 cgtcccggct gacggttctg gccctgcgtt caaccgagcg tgcctttcag ccactgatct
      601 cgcccctgcg gtcttcgctg ctgtatatgg ttctgctggg gcttctcggc cttttggctc
      661 tgctgatctt cgcgctcgag aacagtcgtt tctatcagca gttgcaggaa accaccgaga
      721 acctgcagaa tctgatcgcc tcgaccccct cccccatcat ttcctggaac cgcgacagca
      781 gtatcaccct gatcaatccg gcgttcgagc agttttccgg gtggtcggcc agcgagctga
      841 tcggccagcg ccccgaattc atctttgcca tcgatgacgc attcctcgac gcctgccagc
      901 gtgccaactg cggcaacaat tggaagagcc tggaactggc tatccgtcac aagaacggga
      961 atcttctctt cggactgtgg aattccgcga acctgtatgg ccctgaaaaa accatcgtcg
     1021 ccaccatagt gcagggaatt gacatcaccg aacgcaagaa ggcagaaaac gtcctgctcg
     1081 aaaccaatcg tcaactcgaa caagccacgg cgcaagccca ggctgcgagt attgcgaaaa
     1141 gtgagtttct ggcaaacatg agtcacgaga ttcgtacccc gatgaatggc gtcatcggca
     1201 tgaccgggtt actgctggaa accaacctca gcgtggaaca gcgatcatac gccgagaccg
     1261 tacgatccag cgcagaatcg ctgctcagcc tgctcaatga catcctcgac ttttccaaga
     1321 tcgaagcccg ccgactgacg cttgaaatac ttcccttcga tctgtccagc ctgctcgatg
     1381 acctcgccga ctcactggct atcagggcac acgacaaggg tgtcgaactg atctgtgcca
     1441 tcgacccggc cgttcccgat ctcgtccagg gcgaccctgg tcgtctgcgt cagatcatca
     1501 acaatcttgc cggcaacgcc atcaagttca cgcgcaaggg agaggtgatc ctgcgggtga
     1561 cggtcgcgga agaccagaga gactacgtca ttctgcggtt ctcgatcagc gataccggca
     1621 tcggtattcc gcgtgagaag cagagtttat tgttcgaaaa attttcacag gttgatacct
     1681 ccgtgacccg taaatacggg ggaaccggtc tggggttggc catctcgaaa caattgtccg
     1741 aattattcaa cggaaacatc ggcttcaaca gtgaagaagg gaaagggtca gaattctggt
     1801 tcaccgcccg attacagaaa atgcaggatg gtgcgatcgc ttccattgaa aagacagcaa
     1861 ccctgcaggg tgtgtcggta ctgatcgtcg atgacaacca aaccaatgtc gacttcctgt
     1921 ccgcacgcct ccaatcctgg ggcatgaaaa ccaccgcggt tctcacgggc accgacgctc
     1981 tggaacaact ccggcgggcg catgaggccg gcacgccgtt tccgttggcc attctccata
     2041 tgcagatgcc gggcatggat ggcaaaacac ttggacgtgc gattctggct gatccga
//
LOCUS       DAORHD010000942         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66755_length_2096_cov_5.087212, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000942 DAORHD010000000
VERSION     DAORHD010000942.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66755_length_2096_cov_5.087212"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKO06_17280"
     CDS             complement(<1..312)
                     /locus_tag="PKO06_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71460.1"
                     /translation="MTTTPPVRITGLGKMYRLYEHPWDMLRELWHGHGQTKDFWALQD
                     VSFDVNQGEVFGIIGRNGAGKSTLLRILAGTLDHTLGEVQVAGRISAILELGTGFHPD
                     YT"
     gene            complement(309..1097)
                     /locus_tag="PKO06_17285"
     CDS             complement(309..1097)
                     /locus_tag="PKO06_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71461.1"
                     /translation="MFQQAMQHRHLIWELAMREFRDRFVGHLLGWCWVVLQPALFMGI
                     QIFVFACVFRVSLAGFPHLPHTYATYVLAGMIPWYYVQEMLMKGCVAVSGQAGLVKQV
                     VFPVEVLPLKTVVATTLPQIIAIGLYMLYTFVSFHSLPWTYLLLPIALLLQFLLMSGL
                     SCLLSAVGVYVRDIREVIQSLLLAALYLLPVFYLPDMVPGVFRWVLWLNPFSYLVWVY
                     QDVLYFGHIAHPWAWGVLTVESFIFFVLGAQVFRRLKIMFGSYL"
     gene            1178..1915
                     /locus_tag="PKO06_17290"
     CDS             1178..1915
                     /locus_tag="PKO06_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV71462.1"
                     /translation="MQLSLDHPDLTRQRRQIFGTRPVLKAIYTDWYRRLLSQVPAGDG
                     RILEIGSGGGFLEELCPEVIRSDILPLDHLDVQVHATSGLPFRNASLKAVLALNVFHH
                     LARPRVFLTELTRCLRPGGRLCLIEPWNSLWSRFVYTFFHHEAYRPRTRRWEFASTGP
                     LSGANGALPWIIWERDRARFESEFSNLKIQLVQPLSPFSYALSGGFTHGEFLPGSWFA
                     PLMRAEEANLRLRRACGMFALYVVERV"
     gene            1947..>2096
                     /locus_tag="PKO06_17295"
     CDS             1947..>2096
                     /locus_tag="PKO06_17295"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNV71463.1"
                     /translation="MVRGVMMRRIRVIVRGMVQGVGFRYFTVRQAESQGLAGWVENLE
                     DGSVQA"
BASE COUNT          465 a          572 c          647 g          412 t
ORIGIN      
        1 ggtgtagtcg gggtgaaagc cggtgccgag ttcgagaatg gccgaaattc ttcccgcgac
       61 ctgaacctcg ccgagtgtgt ggtcgagagt accggccaga atgcgcagaa gcgtgctttt
      121 acccgcgcca ttgcgaccaa tgattccgaa aacctcgcct tgattgacgt cgaagctgac
      181 atcctggaga gcccagaagt ccttcgtctg tccgtgtccg tgccacagct cccgcaacat
      241 gtcccacggg tgctcgtaga gtcggtacat cttgccgagc cccgtgatgc gtaccggagg
      301 cgtggtggtc atagatagct tccgaacatg atcttgagtc gacgaaagac ctgcgcgccc
      361 agaacgaaga agatgaaaga ctcgacagtc aaaactcccc aggcccaggg gtgcgcgatg
      421 tgaccgaagt agagaacatc ctggtagacc cagacaaggt aactgaacgg gttgagccac
      481 aagacccagc gaaacacgcc gggcaccatg tcaggcagat agaacaccgg caacagatag
      541 agcgcggcga gcaacagcga ctggatgact tcgcggatgt cgcgaacata gacgccgacg
      601 gccgaaagca ggcaggaaag gcccgacatc aacagaaact gaagcaacag ggcgatcggc
      661 agcagcagat acgtccaggg aaggctgtga aacgagacaa aggtgtagag catgtacagg
      721 ccgatggcga tgatctgcgg aagcgtcgtc gccaccacgg ttttcaacgg caaaacctcg
      781 acggggaaaa ccacctgctt cacgaggccg gcctgacccg aaacggcgac acagcctttc
      841 atcagcatct cctgcacgta ataccaggga atcatgccgg caagtacata cgtcgcgtag
      901 gtatgcggca gatggggaaa accggccaat gagacacgga agacgcaggc gaagacgaag
      961 atctgaattc ccatgaacag cgctggttgc agcacgaccc agcaccaccc cagcagatga
     1021 ccgacgaatc ggtcgcggaa ctctcgcatc gcgagttccc agatgagatg gcggtgctgc
     1081 atggcttgtt gaaacatgcg gcctattcta tggcaccttt ctcaccatcg caatgccgtt
     1141 gggaatgttc gagggcgatg cggtatagtg gaggctcatg caactttcac tcgatcaccc
     1201 agacctgacg cgtcagcgcc ggcagatctt cggcaccagg ccggtgctca aagccattta
     1261 caccgactgg tatcgccgcc tgctcagtca ggtgcccgcc ggcgacggcc ggattcttga
     1321 aatcggctcg ggcggcggat ttctggaaga gctgtgtccg gaagtcattc ggtcggacat
     1381 tcttccgctc gatcatctgg atgtgcaggt gcatgcgact tccgggttgc catttcggaa
     1441 tgcctcgctg aaagcggtgc tggccctgaa cgtgtttcat catctggctc gtccccgcgt
     1501 gtttctcacc gagttaacgc gatgcctgcg gccgggcggg agactctgcc tgatcgaacc
     1561 ctggaacagc ctctggtcgc gttttgtcta cacgttcttc catcatgagg cgtatcgacc
     1621 gaggacgcga cggtgggagt tcgcctcgac cggaccgctg tccggagcga acggcgcact
     1681 gccctggatc atctgggaac gtgatcgcgc gagattcgag tccgagtttt cgaatttgaa
     1741 aatccaactg gtgcagccgt tatcgccgtt ttcgtatgcc ttgtcgggtg ggttcactca
     1801 cggtgagttt ctgcccgggt catggtttgc tccgctcatg cgtgccgagg aagcaaatct
     1861 ccggttgcgg cgtgcctgcg ggatgttcgc tctctatgtg gttgaacgcg tctgaagcgg
     1921 ttgccagacg aagataaccg gtacttatgg tgagaggtgt gatgatgcga cggatacggg
     1981 tgatcgtgcg gggaatggtt cagggagtcg gttttcggta tttcaccgtg cggcaggcgg
     2041 aaagccaggg gttggccggt tgggtggaaa acctggaaga tggttccgta caggct
//
LOCUS       DAORHD010000943         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66820_length_2095_cov_3.209314, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000943 DAORHD010000000
VERSION     DAORHD010000943.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66820_length_2095_cov_3.209314"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PKO06_17300"
     CDS             <1..730
                     /locus_tag="PKO06_17300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV71464.1"
                     /translation="ACRALLCETDDGRRLLFETGIGNFFEPKLRERFGVTPDHHQLLH
                     ELKALGIAPESITDVILSHLHFDHSGGVVERSADGALRLAFPKARFYVGETQWQRAIK
                     PHARDKASYIPEINALLESSGRLVRVTDASYSDLKPLVTFSFSDGHTPGLMLSTLHLP
                     KGPLVFVADLIPASPWINAAITMGYDRFPERLIEEKHALLTDLHAKNGALFFTHDPSM
                     PFARITKDDKGRFVPHSITPADLV"
     gene            852..1271
                     /locus_tag="PKO06_17305"
     CDS             852..1271
                     /locus_tag="PKO06_17305"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV71465.1"
                     /translation="MKDLKPTILELIKKHHLAGLATVTEEGKPWVRYVMLNVADDLTL
                     RTATFRNSRKVAHVKKNPEVHVTCGYDGDMMSPYLQIQAKAEVTDDKKERHALWNDHF
                     KAYFKGPDDPNYVIMIIKPYRIELVEDASHQPKVWTK"
     gene            1516..>2095
                     /locus_tag="PKO06_17310"
     CDS             1516..>2095
                     /locus_tag="PKO06_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71466.1"
                     /translation="MATTTSVVLPYAPSFSFSPEAIRRIGSEACRLCTEHLDRIVQVR
                     AHQKTFDNTFLAFENAIDFFNSTVSVPQFLARVHPDKEVRRAAASLASLAQRHLASML
                     SRVDVYKTLQETFRENPPESESDRFLARQILRDFERNGVNLPTNKREEYQGLKVRLGK
                     LESSFLTAYSRWQGCLTVSASSLNGLSDLFIKS"
BASE COUNT          460 a          625 c          550 g          460 t
ORIGIN      
        1 ggcctgccgc gctctgctct gcgagaccga cgacgggcgt cgtctgctgt tcgagaccgg
       61 catcggcaat ttcttcgagc caaagctccg agagcgtttc ggggtcaccc ccgaccatca
      121 ccagttgttg cacgaactga aggcactggg catcgcgccc gagtcgatca cggatgtgat
      181 tctgtcgcac ctgcacttcg accattccgg cggtgtggtt gagcgaagtg ccgacggcgc
      241 gctccggctc gcctttccga aggcgcggtt ctacgtgggt gagacccagt ggcagagggc
      301 gatcaaaccg catgcgcgcg acaaagcctc gtatattccc gagatcaatg ccctgctcga
      361 gtcatccggc aggcttgtac gagttacaga tgcttcatac agtgacctga agccactggt
      421 caccttctcg ttttctgacg gccacacgcc cgggttgatg ctctcgactc tgcaccttcc
      481 caaggggccg ctggttttcg tcgccgatct gattccggcg tcgccctgga tcaacgcggc
      541 gatcaccatg ggctacgatc gctttcccga gcggttgatc gaagagaagc acgccctgct
      601 caccgacctg cacgcgaaga acggcgcgct tttcttcacc catgacccat ccatgccctt
      661 cgcccgcatc acgaaggatg acaaagggcg ttttgtcccc cactccatca cccccgccga
      721 cctggtttga actcaggagc cggttcactc aatccgagca ggagacggag cccgttcgtt
      781 tgacacggtc agcggattcg cggtaattct cacgtgtccg gatggaaatc gtttttatcc
      841 aaggagttcc catgaaggat ctgaaaccga caattctcga gctgatcaag aagcaccatc
      901 tggccgggct ggccaccgtc accgaagaag gcaagccgtg ggttcgctac gtcatgctga
      961 acgtggccga cgatctcacc ctgcggaccg cgacgttcag gaattcgcgc aaggtggctc
     1021 acgtgaagaa gaatcctgaa gtgcatgtta cctgcggcta tgacggcgat atgatgtccc
     1081 cgtatctgca gattcaagcc aaagccgaag tgaccgacga caagaaagag cggcatgcgc
     1141 tctggaacga tcatttcaag gcatacttca aaggcccgga tgacccgaac tacgtgatca
     1201 tgatcatcaa accctaccgc atcgaactcg tcgaagacgc ctctcaccaa ccaaaggtct
     1261 ggacgaagta acaaccgaca tccgaatctt catttgccaa tcttccagcg gatcggaaca
     1321 gtcggtgatc ctgtgataca tcctgcctgg acgaagcagt tcagttccgg atacacgaca
     1381 gggaacacag tgagaaacca aggttatcga gagtcgttcg ttatctgtct ggtatggatc
     1441 ctctgttcta tcagcgtcaa cccgttgtca gcagcggcga atcgcgcacg caggtcgaaa
     1501 cacccagttg taccagtggc gacaacgact tcggtggtcc tgccgtatgc gccatcattt
     1561 tccttttctc ccgaggcaat caggcgcatc ggcagcgaag catgccgtct ttgtactgag
     1621 catctcgatc gtattgtcca ggttcgcgca caccagaaaa ctttcgacaa caccttttta
     1681 gccttcgaga atgcgatcga tttctttaac agtaccgttt ccgtgccaca gtttctcgcg
     1741 cgcgttcacc ctgacaaaga ggttcggcgt gccgctgctt ctcttgccag cctcgcgcaa
     1801 cgtcatttgg caagcatgtt gtcgagagtt gatgtgtaca agacactcca ggagaccttt
     1861 cgcgagaacc cgccagagtc agaatctgac cgctttctcg cccgacagat tctgcgggac
     1921 tttgaacgaa acggggtcaa ccttccgaca aacaaacgcg aagaatacca ggggttgaaa
     1981 gttcgactgg gaaagctcga gtcgtcattt ctcaccgcgt attcgcgctg gcagggttgt
     2041 ctcaccgttt ctgcatcatc gctgaatggg ttgtcagatc tgttcatcaa atctc
//
LOCUS       DAORHD010000944         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66880_length_2094_cov_3.206964, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000944 DAORHD010000000
VERSION     DAORHD010000944.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66880_length_2094_cov_3.206964"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKO06_17315"
     CDS             <1..333
                     /locus_tag="PKO06_17315"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNV71467.1"
                     /translation="NLKGIAAVIDKDYASALLAQRISADVFIISTAVEKIYLNYGKPD
                     QKALEKCTMAELEKYAAEGHFKPGSMLPKVKAVINFLRQGGKHAIVTNPENLARAVKG
                     EAGTHVYP"
     gene            472..1956
                     /locus_tag="PKO06_17320"
     CDS             472..1956
                     /locus_tag="PKO06_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNV71468.1"
                     /translation="MAKYTKRFELKFRPEVIEKTVKRCRDKGIIIPTFAQMKNPALIP
                     DKVKQKLKPLGLWDVHPLNLFRINWHNDVNSGLYGGVNYLELPSSLTGVKARIIGIVG
                     KFFPTGAHKVGAAFGCLVPRLVSGEFDPTTQKAVWPSTGNYCRGGAYDCALLDAPAIA
                     ILPQDMSKERFDWLKEIGTSEIFATPGCESNVKEIYDKCWELRKERGNGIVIFNQFEE
                     FGNCSWHYHVTAAAVQEVFNQIRKPQTRLSGWVSATGSAGTIGAGDYLKKVSPSLRIV
                     ASEALQCPTLLNCGFGGHRIEGIGDKHVPWVHNVRNTDLVTAVDDENCMRIFRLFNEP
                     AGREALKAMNVPADVIEKLPLLGISSISNMLSAIKAAKFYDMDENDVLFMPFTDAANM
                     YASRLPELTHQLGAYDKARAAIDWERYLLGENIQHSMELTYADRKRLHHFKYYTWVEQ
                     QGKTVDEINELWDPDFWEEAYAQVPQWDKLIAEFNDKTGVLKSL"
BASE COUNT          458 a          667 c          576 g          393 t
ORIGIN      
        1 aatctgaagg gcatcgccgc ggtcatcgac aaagactatg cttcggctct gctggcacag
       61 cgcatcagcg ccgacgtctt catcatctcg acggccgtcg agaagatcta tctgaactac
      121 ggcaagcctg atcagaaagc cctcgagaaa tgtaccatgg ccgagctcga gaaatacgcg
      181 gccgaagggc atttcaagcc gggttcgatg ttgcccaagg tcaaggcggt catcaacttc
      241 ctgcgccagg gcggcaagca cgccatcgtc accaaccccg aaaacctcgc tcgggccgtg
      301 aagggcgaag ccggcaccca cgtctatccg taatgagcga accgagcccg gtgccccagg
      361 tgccgggctt ttcctttctg ccccatgtct tgcaggtgat cgctggaagc gaacacaagt
      421 gaagcggtgt gagtggagca agcacctgca tctaaccaag gagattgagt tgtggccaag
      481 tatacgaaac gttttgaact gaagttccgc cccgaagtta tcgagaaaac cgtgaagcgc
      541 tgccgcgaca agggcatcat cattcccacc ttcgcgcaga tgaagaaccc cgcgttgatt
      601 cccgacaagg tcaagcagaa gctcaagccg ctcggcctgt gggatgtgca tccgctgaac
      661 ctcttccgca tcaactggca caacgacgta aactcgggtc tgtacggcgg ggtcaattat
      721 ctcgagcttc cgtccagtct gaccggcgtg aaagcccgca tcatcggcat cgtcggcaag
      781 ttctttccga ccggcgctca caaggtcggc gcggcctttg gttgtctcgt tccgcgcctg
      841 gtcagcggcg aattcgaccc gaccacccag aaggctgtct ggcccagcac cggtaactac
      901 tgccgcggcg gggcctacga ctgcgctctg ctcgacgcgc ccgccatcgc cattctgccg
      961 caggatatga gcaaggaacg cttcgactgg ttgaaagaga tcggaaccag cgagatcttc
     1021 gccactcccg gctgtgaatc gaacgtaaaa gagatctacg acaagtgctg ggaactgcgc
     1081 aaagaacgcg gcaacggcat cgtcatcttc aaccagttcg aggaattcgg taactgcagc
     1141 tggcactatc acgtcaccgc cgccgcggtg caggaagtct tcaaccagat ccgcaagccc
     1201 cagacccgcc tttccggctg ggtttcggcg accggttcgg ccggtacgat cggcgcgggc
     1261 gactacctca aaaaggtgtc cccctctctg cgcatcgtcg cgtccgaggc gctgcagtgc
     1321 cccaccctct tgaactgcgg gttcggcggt catcgcatcg aaggcatcgg cgacaagcac
     1381 gtcccctggg ttcataacgt gcgcaatacc gatctggtca ccgccgtcga tgacgagaac
     1441 tgcatgcgca tcttccgcct gttcaacgag ccggccggtc gggaagcact gaaagccatg
     1501 aatgtaccag ccgacgtgat tgagaaactg cctctgctcg gcatcagctc gatcagcaat
     1561 atgctctcgg ccatcaaggc cgccaagttc tacgacatgg acgagaacga cgttctgttc
     1621 atgcccttca ccgatgcagc caacatgtac gcttcccgct tgcccgagct cactcatcag
     1681 ctcggcgcgt atgacaaggc ccgcgctgcc atcgactggg aacgctacct gctcggcgag
     1741 aacatccagc acagcatgga actcacctac gccgaccgca agcgtctgca ccacttcaag
     1801 tattacacct gggtcgaaca gcagggcaaa accgtcgacg agatcaacga actctgggat
     1861 cccgacttct gggaagaagc ctacgcccag gttccgcagt gggacaaact catcgccgag
     1921 ttcaacgaca agaccggcgt gctcaagagc ctgtaatcga tatcggggcg gtcgccggtc
     1981 ggccgctccg aaagcctctc acaccgggtg tggcctcacg gccccgcccg gttttttctt
     2041 tctatctcgc cgaatcctgc agccagtttt caggtgttct ggccgtatcc ggaa
//
LOCUS       DAORHD010000945         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66885_length_2094_cov_2.913683, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000945 DAORHD010000000
VERSION     DAORHD010000945.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66885_length_2094_cov_2.913683"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="PKO06_17325"
     CDS             <1..477
                     /locus_tag="PKO06_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71469.1"
                     /translation="EMLSGTYKYIIVDTPPHIGEVELALFDRTDLLLLVATLEISSLK
                     NTKLCLKTLSDIKYETTKTKLLLNKDIPNVGISAKEIEEKLAIPMFGVVPMDSETVQL
                     SLNQGEPFVIKSLDSRLTSAVFQITEKMLEHFDDKPVAAPKKVSAFFKIKELIMGS"
     gene            495..1865
                     /locus_tag="PKO06_17330"
     CDS             495..1865
                     /locus_tag="PKO06_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNV71470.1"
                     /translation="MGGLLARLNEEKKAENKSGIMDSALTREITGFERLKDRVHTKLI
                     DDMPQAIMAEQNQEKKRAMLRDRVIQVTNEESPKLNVILSPREVETLTQILLDDMVGF
                     GPIQKLLDEEDISEIMVNGPFQIYYEKKGKLTLSDIKFKDNDHVMRIIERIVAPIGRR
                     IDESVPYVDARLPDGSRVNAIIPPLALNGPTITIRKFKKEKLGIADMVRFGTLTEEMA
                     KFLEACIKVRLNIVVSGGTGSGKTTTLNILSSFIPEDERIITCEDAAELQLRQPHVVR
                     LETRPANIEGKGAVPMRELIKNTLRMRPERVIVGECRGGEALDMLQAMNTGHDGSLTT
                     GHANTPRDMLARLETMVLMAGMDLPVRAIREQIASAVHLIVQQSRLKDGSRKITYLTE
                     IQGMEGDKIVMQDIFKFEQTGVDEKGKILGRLRPTGIRPKFASKFEESGMHLPPTIFT
                     DTSKGW"
     gene            1869..>2094
                     /locus_tag="PKO06_17335"
     CDS             1869..>2094
                     /locus_tag="PKO06_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71471.1"
                     /translation="MTIELAFVIVVGVTAMIFVLFLFSIFGKPPAEEVRNRMEQYLVE
                     AEMAAKQYVPPDEEIEREKTDQAVEEGADAW"
BASE COUNT          512 a          575 c          596 g          411 t
ORIGIN      
        1 gaaatgctgt cgggaacgta caagtacatc atcgtcgata ccccgcccca catcggcgaa
       61 gtcgagctgg cgttgtttga tcgcactgac ctgctgctgc tggtcgccac gctcgaaatc
      121 agctcgctga agaatacgaa actgtgtctc aagaccctct ctgatatcaa gtacgagacg
      181 acaaagacga aactgctgtt gaataaagac attccgaatg tcggtatcag cgccaaggag
      241 atcgaagaga agctggcgat tccgatgttc ggcgtggtgc cgatggattc ggaaacggtg
      301 cagctctctc tgaaccaggg cgagcccttc gtgataaaat ctctcgacag ccggctcaca
      361 tcagcggtgt ttcagatcac cgaaaagatg ctggaacact tcgatgacaa gccggttgcc
      421 gcaccaaaga aggtcagtgc gttcttcaag atcaaagaac tgatcatggg atcatgaact
      481 ggaggtaaac attcatgggt ggattgctgg cacgtttgaa cgaagagaag aaagccgaga
      541 ataaatccgg catcatggat tcggccctga cccgggagat caccgggttc gagcggttga
      601 aagatcgggt tcacaccaag ctgatcgacg acatgccgca ggccatcatg gccgagcaga
      661 accaggaaaa gaagcgggcc atgctgcgtg accgcgtcat tcaggtcacc aatgaagaga
      721 gcccgaagct gaacgtcatc ctctcccccc gtgaagttga gactctgacg cagattctgc
      781 tcgatgacat ggtcgggttc ggcccgattc agaagctgct cgacgaagaa gatatctcgg
      841 aaatcatggt caacggacca tttcagatct actacgaaaa gaagggcaaa ctgacccttt
      901 ctgatatcaa attcaaagac aatgatcacg tcatgcgcat catcgagcgc atcgtcgccc
      961 cgatcggccg ccgcatcgac gaatcggtac cgtacgtcga cgcccgtctg cctgacggtt
     1021 cacgcgtcaa cgcgatcatt ccgccgctcg cgctgaacgg gccaacgatc acgatccgca
     1081 agttcaagaa ggaaaagctc gggatcgctg atatggtgcg tttcggcacc ctgaccgaag
     1141 agatggcgaa gtttctcgaa gcctgtatca aggtgcgtct gaacatcgtc gtgtccggcg
     1201 gtaccggttc tggtaaaacg acgacgctga acattctcag ctcgttcatt cccgaagacg
     1261 aacgcatcat cacttgcgaa gacgccgccg aactgcagct gcgtcagccg catgtggtac
     1321 gcctcgaaac ccgcccggcc aacatcgaag gcaaaggcgc ggtgccgatg cgcgaactga
     1381 tcaagaacac gctgcgcatg cgccccgagc gcgttatcgt cggcgaatgc cgcggtggcg
     1441 aagcactcga catgcttcag gccatgaaca cgggtcacga cggctctctg accacgggtc
     1501 acgccaacac gccgcgcgac atgctcgccc gtcttgaaac gatggtgctg atggccggta
     1561 tggatctgcc ggtgcgcgcc attcgcgaac agatcgcctc ggccgtgcat ctgatcgtgc
     1621 agcagtcgcg tctgaaggac ggttcgcgca agatcaccta cctgaccgag attcagggca
     1681 tggaaggcga caagattgtc atgcaggata tcttcaagtt cgagcagacc ggcgtcgacg
     1741 agaagggcaa gattcttggt cgtctgcgcc ccaccggcat tcgcccgaag tttgcgtcga
     1801 agttcgaaga aagcggcatg cacctgcctc cgaccatttt cacggatact tcgaaggggt
     1861 ggtaattcat gacgattgaa ctggcatttg tgatcgtcgt cggggttacc gcgatgatct
     1921 ttgtgctgtt cctgttttcc atctttggga aaccgcctgc cgaagaggtg cgcaaccgca
     1981 tggaacagta tctggtcgaa gcggagatgg ctgccaagca gtatgtgccg cccgacgagg
     2041 aaatcgagcg tgagaaaacc gatcaggccg ttgaagaagg tgctgacgcc tggg
//
LOCUS       DAORHD010000946         2093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66921_length_2093_cov_3.451914, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000946 DAORHD010000000
VERSION     DAORHD010000946.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66921_length_2093_cov_3.451914"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PKO06_17340"
     CDS             complement(<1..317)
                     /locus_tag="PKO06_17340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71472.1"
                     /translation="MTFWSVIRLALISLGRNKTRSFLTTLGIIIGVGSVITMVGLGLG
                     AYYSVQDEISKMGTSLLMVMPGSPNSRGSHGPPGTMTNMTEDDAYAIAQECPAVRYVC
                     PTVR"
     gene            complement(314..1054)
                     /locus_tag="PKO06_17345"
     CDS             complement(314..1054)
                     /locus_tag="PKO06_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71473.1"
                     /translation="MEGVVVRALRGVSLKIMPGEFVAIMGASGSGKSTLMNILGCLDR
                     PTKGQYLIEGSDVSGFDKNQLAEVRNRKIGFVFQGFNLLSRTSALENVELPMIYAGVS
                     ASERLQRATEALASVGLAGREQHHPNQLSGGQQQRVAIARSLVNRPPLILADEPTGNL
                     DSRSSVEIMGIFQELNRKGITIVLITHEADIAAFAGRQILVRDGLVVSDLHNEHSQNA
                     AAVLAKMPKLVDAEPDDEPSAQETGGRS"
     gene            complement(1136..>2093)
                     /locus_tag="PKO06_17350"
     CDS             complement(1136..>2093)
                     /locus_tag="PKO06_17350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV71474.1"
                     /translation="DAGRNLKRVTELHQRKLVSQADLDSAQTAVDSAAAAVDAAEAQV
                     KATLSKEESVKAQIQAVTAQHEGSLAQIEQMTAQLNVAKINLERTRIYSPVDGVVISR
                     NVDVGQTVAASLQAPTLFTIANDLRQMQINTAVDEADIGKVREGQKVFFTVDAYRGRE
                     FVGTVGQVRLSPTVTSNVVTYSVMVNVENTDLSLKPGMTASVEILVDEKVNVLRIPAR
                     AQFFRMPDEFLAKVASDPAREDKPDVTRIWVLKHHRPEPRDVVIGLSTSQLAEVVGGD
                     LGVGERVIIDTRKPGEAATTNGGNSSGMRSGMRDYRRMSRGR"
BASE COUNT          462 a          625 c          544 g          462 t
ORIGIN      
        1 cgcacggttg ggcagacata acgcaccgcc gggcactcct gcgcaatggc atacgcgtcg
       61 tcttcggtca tgttggtcat cgttcccggc ggaccatgac tgccacgcga gttcggcgaa
      121 ccaggcatga ccatcagcag gctggtaccc attttcgaaa tctcatcctg caccgaatag
      181 taggcgccca agccgagacc aaccatggta atcacagaac caacaccgat aatgatgccc
      241 aacgtggtca aaaacgacct ggttttgttg cgaccaaggc tgatcaatgc aagccgaata
      301 acgctccaga aggtcatgag cgtcctccag tctcttgtgc ggacggttca tcatcgggct
      361 cagcgtcgac caactttggc attttcgcca acacggcggc cgcattttgt gaatgttcgt
      421 tgtgaaggtc agagaccacc aggccatcgc gcaccaggat ctgtcggccg gcaaaagcag
      481 cgatatccgc ttcgtgcgtg ataaggacga tagtgattcc cttccgattc agttcctgaa
      541 agatgcccat gatctccacg ctcgaacgcg aatcgaggtt ccccgtcggc tcgtcggcaa
      601 gaatcaaggg ggggcgattc acaagcgatc gcgcaatcgc cacgcgttgt tgctgcccac
      661 cggaaagctg atttgggtga tgctgttctc gtccggccag gccgaccgaa gccaaagcct
      721 cggttgcccg ctgcaggcgt tccgaagcgg aaacaccagc gtaaatcatc ggcaactcga
      781 cgttctcgag tgcactggtg cgggaaagca ggttgaagcc ctgaaagaca aatccgatct
      841 ttcggttgcg cacttccgcc agttgattct tgtcgaatcc tgagacgtcg ctgccttcga
      901 tgagatactg ccccttcgtc ggccgatcaa gacagccgag aatgttcatc aacgttgatt
      961 ttcctgagcc agaagctccc atgatggcca cgaattcgcc cggcatgatt ttcagcgata
     1021 cacctctcag cgcacgaaca accacacctt ccatgaaata cgtcttctgg acgtgcacga
     1081 cctccagcaa aggagcaaca gccgcttctg aagacattct tacgtccttt gtcgatcagc
     1141 gaccgcgact catgcgccgg taatcgcgca tgccgctgcg catacccgat gagtttccac
     1201 cattggtcgt tgcggcttcg ccaggttttc gggtatcgat aatcacccgt tcgcccaccc
     1261 caagatcacc accaaccacc tcggcaagct gacttgtcga gagcccgatc accacgtcac
     1321 gcggttcggg gcgatggtgc ttgagcaccc agatacgcgt cacatcaggt ttgtcttcgc
     1381 gagcggggtc actcgccacc ttcgcgagaa attcgtcagg catgcggaaa aactgcgctc
     1441 gagctggaat ccgcaggacg ttaaccttct catctaccag aatctctacg ctggcggtca
     1501 tgccaggctt cagtgaaaga tcggtgtttt cgacgttcac catcacggaa taggtcacaa
     1561 cattggaagt cacggttgga gacaggcgta cctggccgac tgtaccaaca aactcacgac
     1621 cacgataggc atcaaccgtg aagaagactt tctgtccttc acgcactttc ccgatatccg
     1681 cctcgtctac cgctgtattg atctgcatct ggcgcagatc gttggcgatt gtaaacaacg
     1741 tcggggcctg cagactggcc gccacggtct ggccgacatc aacattccgg gaaatgacca
     1801 ctccgtcaac cggagaatag atacgcgtac gttcgagatt gattttggcc acattcaatt
     1861 gagccgtcat ctgctcaatc tgtgcgagag atccctcatg ctgggccgtg accgcctgaa
     1921 tctgagcttt gaccgattct tccttgctaa gggtggcttt gacttgcgct tcagctgcat
     1981 caacagcggc tgctgctgaa tcgacagcgg tctgtgcgct gtcgagatcc gcttgcgata
     2041 ccagtttgcg ctggtgcagt tccgtgaccc gtttgagatt gcgtccagcg tct
//
LOCUS       DAORHD010000947         2092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_66971_length_2092_cov_3.588611, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000947 DAORHD010000000
VERSION     DAORHD010000947.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66971_length_2092_cov_3.588611"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="PKO06_17355"
     CDS             <1..1104
                     /locus_tag="PKO06_17355"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71475.1"
                     /translation="PLMTTSSAQLLTHEEVDFGVIGEGELTLHQLIEHTTGDLSGIAG
                     LAWKKNGEIRHNDPRQPIACLDELPFLDYSLVPTQVYLDFQQRLEMPPWLFLSTSRGC
                     PFRCTFCATPALWPGPVRRLSVPRLFKEMAHHRSRFPDAHFGFMDDSFFSDKRWLHQF
                     FDAMANFGGRYCCIGRADHLEAGDVTRLAQTGCHYVALGVETGNQNRQKKIRKYLDLR
                     KVERSVALLIQHGILCKCFFMLGFPDETLEEMVETVNFAVTCARLGMQECNIFPVSAY
                     PGTELAHGLDHSVFRSTIYHGHDHDNRPITEFESSEARSEKRLSIYAAVPDADLNPYV
                     NRQQLLELIRLAYQKVERREDLTLSEIQEIQNR"
     gene            complement(1117..1968)
                     /gene="phnD"
                     /locus_tag="PKO06_17360"
     CDS             complement(1117..1968)
                     /gene="phnD"
                     /locus_tag="PKO06_17360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV71476.1"
                     /translation="MMRLVFRLVLMVILPWIALDSRIQAQEDLQVICLPQPPLRFAVY
                     PFLERLTLRERLQPLLQRLAGELGRPVVPVMASDYSQLERLLIKRQAHLCWYSPRRSS
                     PSLREMGLLAVCRPTLREGQAYRGVIVARRDRAAASLSDLRGCRFAYIDRQSNSGFLY
                     PNRLFEEHGILPLEFFGEIHFAGTHDAALLGVAHGLYDAAAVTDLVLERGPLNESERS
                     RLMVLATTTPILPDVIAVSETLDPALRKQITDFFLGLNTSLASQTALDEVLTPLGFCG
                     FVPVDSN"
BASE COUNT          506 a          600 c          557 g          429 t
ORIGIN      
        1 ccgctgatga cgacttcttc agcccaactg ctcacgcacg aagaagttga cttcggcgtg
       61 atcggggaag gcgaactcac actgcatcag ctcatcgagc acacaacagg cgacctgtca
      121 ggtattgccg gtctggcctg gaaaaagaat ggtgagatca ggcacaatga cccgcgtcag
      181 ccgatcgcct gcctggacga gctaccgttt cttgattatt ccctggtgcc cactcaggtc
      241 tatctcgatt ttcagcagcg tctcgaaatg ccgccgtggc tgttcttgag cacttcccgc
      301 ggatgtccgt ttcgctgcac gttttgcgcc accccagcct tgtggcctgg accggtacgc
      361 cgactttcag tgcctcgcct gttcaaagaa atggcgcatc atcgatcgcg gtttccggat
      421 gcgcattttg ggttcatgga tgacagcttc ttttccgaca agcgatggct gcaccaattt
      481 tttgatgcca tggcaaactt cggaggccgg tactgctgca tcggacgtgc agaccatctg
      541 gaagcaggcg acgtcacgcg tctggcgcaa accggttgtc attacgtggc gctcggcgtc
      601 gaaaccggaa atcagaaccg gcagaaaaag attcgcaagt atctcgacct gcggaaagtc
      661 gaacgcagcg tggcgttgct gattcagcat ggcatactct gcaaatgctt tttcatgctg
      721 ggtttcccag acgaaaccct tgaggagatg gtggagaccg tcaatttcgc cgtaacctgt
      781 gcccggcttg gcatgcagga atgcaacatc tttccggtct cggcctaccc cggaaccgag
      841 ttggcgcatg ggctcgacca ctcggtcttt cgttcgacga tttaccacgg tcatgatcac
      901 gacaatcggc cgatcaccga gttcgagagt tcagaagcac gcagcgagaa gcgtctttcc
      961 atttacgctg cagtaccaga tgccgacctc aatccgtatg tcaatcgtca acaactgctc
     1021 gagctgatcc gtcttgccta tcagaaagtg gagcgccgtg aagatctgac gctctcagaa
     1081 attcaagaaa ttcagaaccg ttgaacagtc gttatctcag ttagaatcta ctgggacaaa
     1141 tccgcaaaat ccaagcgggg tcaacacttc gtcaagtgcc gtctgcgaag ccagcgacgt
     1201 attcagtcca agaaagaaat cggtgatctg ctttctcaga gcgggatcaa gcgtctcaga
     1261 cacggcgatg acatcgggaa gaatcggcgt ggtggtcgcc agcaccatca gacgggaacg
     1321 ctcgctttcg ttgagcggcc ctcgttccaa taccaggtca gtgaccgccg cggcatcgta
     1381 caagccatgc gcaactccaa gaagtgccgc atcatgtgtt ccggcaaaat gaatctcacc
     1441 gaagaattcg aggggaagaa ttccgtgttc ctcgaagagt cggttcggat acagaaaccc
     1501 gctgttggat tgccgatcga tgtaggcaaa gcgacagccc cgcagatcag acaaactggc
     1561 agcagctcga tcgcgtcggg ccacaatcac cccgcggtag gcttgtccct cgcgaagagt
     1621 cggccgacac acggcaagca accccatttc tcgcaaggaa ggggagcttc gtcgcggaga
     1681 gtaccagcaa agatgggcct gacgcttgat cagaagacgc tcaagctggc tgtaatcgga
     1741 agccatgacc ggaacgaccg gtcgtccgag ttctcctgcc agcctctgca acagtggctg
     1801 cagcctttca cgcaacgtca gtcgttcgag aaatggatac actgcaaaac gcaacggagg
     1861 ctgtggcaga catataacct gcagatcctc ctgagcctga atccgactgt cgagagcaat
     1921 ccacggcagt atgaccatca gaaccagtct gaaaacaaga cgcatcatgg aaacatcgtc
     1981 tcctcatcaa caaaggaacg gcaggtctca ggggtccaat cgaccaactc gataccggcc
     2041 cagtcacgac agtgatcact caacgacacc cgctcgggtt cgagcagaac gc
//
LOCUS       DAORHD010000948         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67133_length_2089_cov_3.311701, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000948 DAORHD010000000
VERSION     DAORHD010000948.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67133_length_2089_cov_3.311701"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="PKO06_17365"
     CDS             complement(<1..1182)
                     /locus_tag="PKO06_17365"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV71477.1"
                     /translation="MNHMRKHHVVSVAFIACLALTLLVLSTGCNKAPEKTADGRTILT
                     FWHTYGDQEEAVLKEIIATWEAKNASWSVQALRLPFDGHKPKIRTALTVGQGPDMARV
                     DWSYVCELARKNALADLEQFGFSKIRDDYVKAPLNTNFIDGKYRGFPDQTNCLCLFYN
                     KQLFREAGLDPETPPKTWEKFVEMGKKITNPEKGVYAFAMDNTVWWTLPFFNSFGARL
                     ISEDGKTCLLDAKEAVDAVSFKAGLYTQHKIEAGAWKAGSIPPESGFINKKYAMIFMG
                     PWNLPKFARSDVDFGVTLIPAGPAGSSSNVGGTNVVIFNHGKYQQACYDFLTFFTSAE
                     SQAKWCTKLNQIPVNIKAYDLVKFDNPHLMVFMEQMKTAVANPIVTQFDKLEDIVNPE
                     ME"
     gene            complement(1193..>2089)
                     /locus_tag="PKO06_17370"
     CDS             complement(1193..>2089)
                     /locus_tag="PKO06_17370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV71478.1"
                     /translation="PEHGRDEFIKMLFLYQALSVGVVVVLFPFFWMICTAFKPLGTEL
                     KFDTANPFDIVPTDPTISNFRKVLKLESAAPGAPVDPEESKKNFGRYFWNSMVVSVSA
                     AFLVTLFAAMGAYVFSKKELPFKKYLFQVLLWTMMIPGMMYMVPQFFMVCKMGLFGTK
                     AAMFLPHLASVFGVFMLKQFMDTIPNSLLEACRIDGASEWQIFRIVIVPLAMPIILTL
                     FLLTFLFHWSNFLWQLIVTDPQDQLSYTLPVALALFRGQYTSDLGLIMSASCFSIVPI
                     VVLFLFAQRYFIEGMTAGSVKE"
BASE COUNT          479 a          594 c          578 g          438 t
ORIGIN      
        1 ttccatttcg gggttcacga tgtcttcgag cttgtcgaac tgggtgacga tcggattcgc
       61 cacagccgtc ttcatctgtt ccatgaagac catcaaatgc gggttgtcaa acttcaccag
      121 gtcgtacgct ttgatattga ccggaatctg attcagcttc gtacaccact tcgcctgcga
      181 ctcggccgag gtgaagaagg tcaggaaatc gtagcacgcc tgctggtatt tcccgtggtt
      241 gaagatgacc acgttcgtgc caccgacatt actcgaacta ccggcggggc cggccggaat
      301 cagcgtcacg ccgaagtcga catcgctgcg ggcgaacttc ggcaggttcc agggtcccat
      361 gaagatcatc gcatacttct tgttgatgaa gcccgattcg ggcggaatcg atccggcttt
      421 ccaggccccg gcttcgatct tgtgctgggt gtacaagccg gctttgaaac tgacggcatc
      481 aacggcctct ttcgcatcga gcagacacgt cttgccgtct tcgctgatca ggcgagctcc
      541 gaacgaattg aagaacggca gcgtccacca gaccgtattg tccatggcga aggcatacac
      601 gcccttttcc gggttggtga tcttcttgcc catctcgacg aatttttccc atgtcttcgg
      661 cggcgtttcg gggtcgagac cggcctcacg aaacaactgc ttgttgtaaa acaggcacag
      721 acagttggtc tggtcgggaa agccccggta ctttccatcg atgaagtttg tattcaatgg
      781 tgctttgacg tagtcatcgc ggatcttcga gaagccgaac tgttcgagat cagcgagcgc
      841 atttttccgc gccagttcgc acacgtacga ccagtcgacg cgcgccatat caggcccctg
      901 ccccaccgtc aacgccgtcc gaatcttcgg cttgtggccg tcgaacggca gccgcaatgc
      961 ctgaaccgac caactggcat tcttcgcttc ccaggtggct atgatctctt tcagcaccgc
     1021 ttcctcctgg tcgccgtagg tgtgccagaa ggtcagaatg gtgcgaccgt ccgccgtctt
     1081 ctcgggggcc ttgttgcacc cggtcgaaag taccagcagc gtcaaagcaa gacaggcaat
     1141 gaaagcgaca gaaacaacgt ggtgttttcg catgtggttc atggtcgccc cctcactctt
     1201 tcaccgagcc ggcggtcatg ccctcaatga agtagcgctg agcaaacaga aagagcacga
     1261 cgatcggtac gatcgaaaag cacgatgccg acatgatcag accaagatct gacgtgtact
     1321 gtccgcggaa caacgccagc gccaccggca gggtgtagct caattggtcc tgcggatcgg
     1381 taacaatcag ctgccacaga aagttcgacc agtgaaagag gaacgtcagc aggaacaacg
     1441 tgagaatgat cggcatcgca agcggcacga tcacgattct gaagatctgc cactcggacg
     1501 cgccatcaat gcggcaggct tcgagcagcg agttcgggat cgtatccatg aactgcttga
     1561 gcatgaagac gccgaagacc gaggccagat gtggcagaaa catggcggcc ttcgttccga
     1621 acagccccat tttacagacc atgaagaact gcggcaccat gtacatcatg ccgggaatca
     1681 tcattgtcca gagcagaacc tgaaacagat acttcttgaa cggcagttcc tttttcgaaa
     1741 agacgtaagc ccccatcgcg gcaaacaggg tcacgagaaa ggccgcggag accgagacga
     1801 ccatgctgtt ccagaagtag cgaccgaagt tcttcttcga ctcttcgggg tcgaccggcg
     1861 cgccgggggc cgccgactca agcttgagca ccttgcggaa gttactgatc gtcggatcgg
     1921 ttggcacgat gtcgaatggg ttggcggtat cgaacttcaa ttcggtccca agcggtttga
     1981 aggccgtgca gatcatccag aagaagggaa agagcaccac gaccacgccc accgagagcg
     2041 cctggtacag aaagagcatc ttgatgaact cgtcgcgtcc atgttccgg
//
LOCUS       DAORHD010000949         2087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67247_length_2087_cov_2.934547, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000949 DAORHD010000000
VERSION     DAORHD010000949.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67247_length_2087_cov_2.934547"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKO06_17375"
     CDS             <1..329
                     /locus_tag="PKO06_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71479.1"
                     /translation="AYTVAQETLELVGNIGYPKLRTLVLTNDSVLQKLGYSSFQQVVD
                     IPLAGSDKLAYPEEYKRLERKIEITPSLSTDPPPPLVRVVVTVRVREGFFRRGAIVLG
                     KLVGAE"
     gene            332..985
                     /locus_tag="PKO06_17380"
     CDS             332..985
                     /locus_tag="PKO06_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71480.1"
                     /translation="MIRGSTRSFGVTLVEIMIVLIIGVMLLIPTINMIMSTQRSTFKG
                     YGRLETLTTARIILEKVQRDLKALCYSKSAEAFIVTKTGKITTWTFNAFPTEGFVKTL
                     PGTVNPANLITYRFDEEKKTLVRAVKIHNDLLTGYTGPNPAHEILGKNVVLFQIKDRS
                     LFGSIFYDIDIKCQSDHPMQAKEATHLHSAARSEFEWRLEQNQYQVPNRQSTIDVVK"
     gene            1056..1460
                     /locus_tag="PKO06_17385"
     CDS             1056..1460
                     /locus_tag="PKO06_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71481.1"
                     /translation="MTHVLKRSTFFVCLLAMSLALTGCDIDISKIADFVTKIADVVSK
                     VAEGLKGFASNLKNASSSTSTTDSTSTTAAAPTSTTSPTTTTTTPTTTTTTPTTTTTT
                     PTNVGAGGTDSTTTRPADTSTTGNANTSVTGS"
     gene            complement(1812..>2087)
                     /locus_tag="PKO06_17390"
     CDS             complement(1812..>2087)
                     /locus_tag="PKO06_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71482.1"
                     /translation="VAWLLNKPSSAPRGRMLSLMAELLQAQAVLQHDTTEGMVDYTQN
                     TERNLIQILHENPELLKEAKASPATGGLQNVFRAVETMSTFSTIHHE"
BASE COUNT          486 a          627 c          523 g          451 t
ORIGIN      
        1 tcgcctacac cgtcgcccag gagaccctcg aactggtggg aaacatcgga tatcccaaac
       61 ttcgcacgct ggttctcacc aatgacagcg tgcttcagaa gctcggatat tcctcgtttc
      121 agcaggtggt cgacattccc ctcgccgggt cagacaagct cgcgtatccg gaagagtaca
      181 agcgtcttga acgcaagatc gagatcacgc ccagtttatc gactgatccg ccgccaccac
      241 tggtgcgcgt cgtagttact gtacgagttc gcgaaggctt ttttcggcgc ggagccatcg
      301 ttcttggcaa actggttggt gcagaatgag tgtgatccgc gggtcgactc gctcgttcgg
      361 cgtcactctc gtcgaaatca tgatcgtcct gattatcggg gtcatgttgc tgattccaac
      421 catcaacatg atcatgtcga cgcagcgcag caccttcaag gggtatggac gtctcgaaac
      481 tctgacaacg gctcgcatta ttctcgagaa ggtgcagcgc gatctcaaag cgctttgtta
      541 cagcaagtca gccgaggcgt tcatcgtcac caaaaccgga aagatcacca cctggacatt
      601 caatgccttt cctaccgagg gattcgttaa aactctcccc ggcacagtga accccgccaa
      661 tctcatcacc taccgattcg atgaagagaa aaagacactg gtacgagccg tcaagatcca
      721 caatgatctg cttaccggtt acaccggccc caaccccgcg catgagatcc tggggaaaaa
      781 cgtcgttctc tttcagatca aggaccgcag cctcttcgga tccattttct atgacatcga
      841 tatcaagtgc cagtctgacc atccaatgca ggccaaagag gccacgcatc tgcactcggc
      901 agcccgttcc gaattcgagt ggcgactcga gcagaaccag taccaggtcc ccaatcgcca
      961 atcgaccatc gacgtggtaa aatagcttgt aacattttcg acagcatttc tgtataatca
     1021 ggtacgaaca aaatttcttc cgaggaggag ctcatatgac ccatgttttg aagcgcagta
     1081 cgttctttgt ctgtttgttg gccatgtctc tcgccctgac tggttgtgat atcgacatca
     1141 gcaagattgc cgacttcgtg accaaaatcg ccgacgtggt ttcgaaggtg gctgagggcc
     1201 tgaagggctt tgccagcaac ctgaagaacg cctccagctc aacgagcacg accgacagca
     1261 ccagcaccac cgctgcagcg cccacaagta caacgtcgcc gacaacaacg acgaccactc
     1321 cgaccacaac cacaacgacc ccaacgacaa ccaccaccac tccgaccaat gtcggtgcgg
     1381 gcggaacgga cagcaccacg actcgcccgg cagatacctc cacaacgggg aatgccaaca
     1441 ccagcgtcac cggcagctga tcttcgtctt accagagaac ccccgagcca atggctcggg
     1501 ggttttccat tgtcccgata ccgctcgtcg ggttcgcaag ccttgatttc ttcgggtcga
     1561 ggaacggtct atcgaatcga gacacccact tcgatcatgc caagctgctc atcggctgca
     1621 tctctttgca gatgagcgag agcagttggt acagaaggga tgtcgcgtgt cgtgttccgg
     1681 gttcacctgg cttcaacaac gggtactggg cgttcaatgc tctctacccg tcgggtgcca
     1741 gagctcagcg tcgccaaaga acaaagaaaa aaccgggctt tcgcccggct tgtttcgacc
     1801 tcgttgaacg cttactcgtg gtgaatcgta gagaaggtgc tcattgtctc gactgcccga
     1861 aacacgttct gaaggccacc agtcgccggt gatgccttgg cctctttgag cagctcggga
     1921 ttttcgtgaa ggatctgaat cagatttcgc tcggtgttct gcgtgtagtc gaccatccct
     1981 tcggtcgtat cgtgctgcag caccgcctga gcctgcagca gttccgccat cagactgagc
     2041 attcgaccac ggggggcact actgggcttg ttcagcaacc acgcaac
//
LOCUS       DAORHD010000950         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67287_length_2086_cov_3.587395, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000950 DAORHD010000000
VERSION     DAORHD010000950.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67287_length_2086_cov_3.587395"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1497)
                     /locus_tag="PKO06_17395"
     CDS             complement(<1..1497)
                     /locus_tag="PKO06_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71483.1"
                     /translation="MLSISNSDTLSDVTQKDCAMHNHTVNVHGPRGGFAFAEALVMIM
                     FVGTLMMPMMGSLTNTAARTIDMRHQRLAEAHAQTVQAQLLASAPFRDPPPGATYEFP
                     LDDLSTNTVTVTVRTLVGVSSVSVPPQLLATPTFYHYRIDVVRTSNDAALQSPATMTF
                     LTGLLPAKPVNVNDPPIYICDQVNLKILIVTIDDSSGVPVGTIIDEIPFNKGANCGAM
                     SGDAPSSDYIGHCPGEMAVHPSGKWLFGAGSQFAWIIDVDPASPYYKQEVWHRDPEAN
                     ADGTAIDNLIGLGNSDEGAHLALSPCGKYFHVMTNCSCIYHYTFNSTVDPPTGIFLYK
                     NHDADGSLWSGCNKGVAYSRTGLLAVTRQDSAQHGAGTDNTWYGDPRYIFGGARPAAA
                     TPSRWQKLKIDSGHLDGAAFTFSHDGRFIYRHPDDCDSGWGGYKMDLAFSRIGARLSP
                     SVNSDNWARLALSADGQVMCAPRYEGTNPSFCWYHTNPPDHQLILNSID"
     gene            complement(1503..>2086)
                     /locus_tag="PKO06_17400"
     CDS             complement(1503..>2086)
                     /locus_tag="PKO06_17400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71484.1"
                     /translation="GSNRIKALSAAYAGLNFYQGLLLATTTTFDRTDWNSCRERFHFL
                     ARAPIAQLNNALYPGRQIATSTVGALAAVGDAWYNGFIHVATAQWLFASDPAIIPTDF
                     DVPSSSLFIIKTYVASPAAGIASYVYVKSLGCYREIQNDEIVASYYAQLLARIEISTV
                     TSTLSINRLMQMPLEYPFCATAPFFASMPVPWK"
BASE COUNT          445 a          538 c          690 g          413 t
ORIGIN      
        1 gtcgatcgaa ttcaagatga gctggtgatc aggtggattc gtatgatacc agcagaaact
       61 cgggttcgtg ccttcgtatc gcggggcgca catcacctgt ccgtcagcac tcaacgctaa
      121 tcgcgcccaa ttatccgagt tgaccgaggg cgagagacgc gcgccaatac gagagaacgc
      181 caggtccatt ttgtatccac cccacccgga gtcacaatca tccgggtggc gatagatgaa
      241 gcgtccatcg tgcgaaaagg tgaaggccgc accgtcgagg tgaccggaat cgatcttcaa
      301 cttctgccag cgcgaagggg tcgcggccgc aggacgagcg ccaccgaaaa tgtaacgagg
      361 gtcaccatac caggtgttgt cggttccagc cccgtgctga gccgaatctt gacgggtgac
      421 ggccagcaga ccggtacggc tgtaggcgac gcccttgttg cagccagacc agagagaccc
      481 gtctgcgtcg tggttcttgt agagaaaaat cccggtcgga ggatcgacgg tcgagttgaa
      541 ggtatagtga taaatgcacg aacaattggt catgacgtgg aaatacttgc cgcacggcga
      601 gagcgcgaga tgtgcgcctt catcagagtt gcccaggccg atcaggttgt cgatggctgt
      661 gccgtcggcg ttggcttccg ggtctcgatg ccagacttcc tgtttgtaat acggcgaggc
      721 agggtcgacg tcgatgatcc aggcgaactg actaccggcc ccgaacagcc attttccgga
      781 aggatggaca gccatttcgc cagggcagtg cccgatgtaa tcactgctgg gcgcatcccc
      841 cgacattgcc ccgcagttgg ctcccttgtt gaacggaatc tcgtcaatga tggtaccgac
      901 cggaactccg gagctgtcat cgatggtgac gatgagaatt ttcaggttga cctgatcgca
      961 gatgtagatc ggtggatcgt tgacgttcac tggtttagcg ggaagcaggc cggtgagaaa
     1021 agtcatggtc gcgggcgact gtaatgcggc atcattcgag gtgcgcacga cgtcgatacg
     1081 gtaatgataa aaggtcgggg tggcgagaag ttgtggcgga acactgacac tgctgacccc
     1141 caccaaggtt ctgaccgtga cggtgacggt attggtcgac aggtcgtcga gtgggaactc
     1201 atacgttgcg cccggtggtg ggtcgcggaa gggagccgag gcgagaagtt gagcctgtac
     1261 cgtctgggca tgggcttcgg cgagtcgctg gtggcgcatg tcgatggtgc gcgcggcggt
     1321 gttggtcagt gagcccatca tcggcatcat cagggtgccg acgaacatga tcatcaccag
     1381 cgcttccgcg aacgcgaagc ctccgcgagg gccgtgaacg ttgaccgtgt gattgtgcat
     1441 ggcgcagtcc ttctgtgtga cgtcagacag agtgtccgag ttactgattg acaacacgta
     1501 gatcatttcc agggaacagg catcgaggca aagaacgggg ccgttgcgca gaacgggtac
     1561 tcgagcggca tctgcatgag acggttgatc gacaacgtgc tggtcacggt gctgatctcg
     1621 atgcgggcga gcagttgcgc gtaatacgag gcgacgatct catcgttctg gatctcgcgg
     1681 tagcagccca gcgatttgac gtagacgtat gaagcaatgc cggcggctgg cgaggctacg
     1741 tatgtcttga tgatgaacag tgaggaactg ggtacgtcaa agtccgtggg aatgatcgct
     1801 ggatcgctgg caaacagcca ctgcgcggtc gcgacatgaa tgaagccgtt ataccaggcg
     1861 tcgccgaccg cggcgagcgc tcccacggtc gaggtggcga tctgacgccc cggatagaga
     1921 gcattgttca gttgggcgat cggagcgcgc gccagaaagt gaaaccgctc gcggcacgag
     1981 ttccagtcgg tacggtcaaa ggtggtggtg gtggccagca gcaggccctg gtagaagttc
     2041 aggccggcgt aagccgctga cagagccttg atgcggttgc tgcccg
//
LOCUS       DAORHD010000951         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67303_length_2086_cov_2.887740, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000951 DAORHD010000000
VERSION     DAORHD010000951.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67303_length_2086_cov_2.887740"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..372)
                     /locus_tag="PKO06_17405"
     CDS             complement(220..372)
                     /locus_tag="PKO06_17405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71485.1"
                     /translation="MPYCQRETEVIRDCVLELLICLPTYEGDFFHILSIIDQHQEGDA
                     EQSKPN"
     gene            complement(473..1585)
                     /locus_tag="PKO06_17410"
     CDS             complement(473..1585)
                     /locus_tag="PKO06_17410"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNV71486.1"
                     /translation="MSEARKRILNMVSEGKISVDQAEELLAALETEQKSAPQGESSSD
                     RKKRLGDEIKSFAEQIQREVSQAMKGMNPQTQEIKDNAKKLGVWFQGMVESVTQNFSK
                     TFASSFDGIECDFTLGEPEGFASCKTAEVSNHYGAIQIREGSAFELRVKGRLAKMAFG
                     SQTPQDWFAANGLKVQDGTLIIGIDSFLPTKGGFDFELTIPAHLPLRCRVLSGDLNVT
                     GNLTIERAQSVSGDLAFRQTSLRNCVIETVSGDVRVDEGVVDSDLKTSSGNLQVRRCQ
                     VHKMRAQFISGDIEVLEPKVDDATVIDILTTSGNVCIANPSGGFGKVIANTRTGTLTS
                     GWGGISISEAGNSIRQEGLIGATLLIETISGDIGLR"
     gene            complement(1578..>2086)
                     /locus_tag="PKO06_17415"
     CDS             complement(1578..>2086)
                     /locus_tag="PKO06_17415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNV71487.1"
                     /translation="RSRFSCKHDTLLWYAPHGKPFFNPDPVRQPYLKSSGYARSGIRS
                     ANGKRYLPDPRGTPPDDVWNIPIINPMSSERVGWPTQKPLALLERLILALTEPQALVA
                     DFMCGSGTTLVAAAVHGRRWVGGDRSPEALRCAQTRLAACCKAQIEVLACDTMNDTKI
                     PKEDDENV"
BASE COUNT          454 a          570 c          574 g          488 t
ORIGIN      
        1 cgcggagtga aggggtttct gacatgtggg tgacggctcc ttgaggttgt gaaagagata
       61 acacatctcc cttctcccgg gaaagggcag gtcagaccag acaggaattt tcctggaggt
      121 cttgcggtcg tggtcgctga tcgcggggtg aactggaata cagttactcg tagtgcatga
      181 aacgcggcga ctgaaaagca acaaccagat gatgctcgct taattcggct ttgattgctc
      241 agcatctcct tcctgatgct gatcaataat cgagagaatg tgaaagaagt ccccttcata
      301 ggtcggaagg cagatcagaa gctccaggac gcaatcccga atgacctcgg tttcccgttg
      361 acagtacggc atcgctgaac ctccgggggc cccgctactt tcattgtaaa gagcaaaggc
      421 cgtcttcgta aagaagacgg cccgtttatt ttcgttcaaa aatttttaca gttcagcgca
      481 atccaatatc tccgctgatg gtttcaatga gcagagttgc gccgatcaga ccttcctgcc
      541 gaatgctgtt gccggcttcg ctgatcgaga tgccacccca gccagatgta agagtcccag
      601 ttcgggtgtt tgcaatcacc ttgccaaagc cgcccgacgg gtttgcaatg cagacgttcc
      661 ctgacgtcgt cagaatgtcg atgacggtgg catcatcgac tttcggctca agaacttcaa
      721 tatcgccgga aatgaactgc gcgcgcatct tgtgaacctg acagcggcgc acttgcagat
      781 tgcctgagct ggtcttgagg tcagagtcga caaccccttc atcgactcga acgtcaccgc
      841 tgaccgtctc gatgacgcag ttccgaagtg aggtctggcg aaaggccaga tcaccgctga
      901 cactttgcgc acgctcgatg gtcaggtttc cggtcacgtt cagatctccc gacagaaccc
      961 ggcagcgcag tggtagatgt gccggaatgg tgagttcgaa gtcgaaaccg cctttggtcg
     1021 gaagaaacga atcgatgccg atgatcagtg tgccatcctg aaccttcaga ccgttggcgg
     1081 cgaaccagtc ctggggtgtc tgactgccga aagccatttt ggccagtcgg cctttgactc
     1141 gcaactcgaa cgccgatccc tctcgaatct ggatcgcacc gtaatggttg ctgacttccg
     1201 ccgtcttgca cgatgcaaac ccttctggtt cacccagcgt gaaatcgcac tcgataccgt
     1261 caaaggaact ggcgaacgtc ttcgagaagt tctgggttac cgattcgacc attccctgga
     1321 accagacacc cagcttcttg gcattgtctt tgatttcctg agtctgcggg ttcatcccct
     1381 tcatggcctg gctgacttcg cgctgaattt gttcggcaaa gctcttgatt tcatcaccca
     1441 gccgcttttt gcgatcgctt gaggactcgc cttgtggggc ggatttctgt tctgtttcaa
     1501 gagcagccag aagctcttca gcttgatcga ccgagatctt gccttcggaa accatgttca
     1561 gaatacgttt gcgggcttca gacattctcg tcgtcctcct tggggatttt cgtgtcgttc
     1621 attgtatcac acgcgagaac ctcgatctgc gccttacagc aagctgcgag acgcgtctgt
     1681 gcgcaccgca gagcttctgg cgaacggtct cctccgaccc agcggcggcc atgaactgcc
     1741 gcggcaacca gcgtcgttcc actgccgcac atgaaatcag ccacgagagc ctggggttcg
     1801 gtcagagcca gaatcagtct ctccagcagg gccaagggct tttgggtcgg ccacccgacg
     1861 cgttcggaac tcatggggtt gatgatcgga atattccata cgtcatcagg gggggtgccg
     1921 cgcgggtcag gaagatagcg ttttccattg gctgatctga tgccagaacg cgcatagcca
     1981 gaggatttca gatagggctg acgaaccgga tcaggattga aaaagggctt gccatgcggc
     2041 gcataccaca gcagggtgtc gtgcttgcac gaaaaacgag atcggg
//
LOCUS       DAORHD010000952         2086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67305_length_2086_cov_2.851305, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000952 DAORHD010000000
VERSION     DAORHD010000952.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67305_length_2086_cov_2.851305"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKO06_17420"
     CDS             complement(<1..286)
                     /locus_tag="PKO06_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71488.1"
                     /translation="MRHRFRVGNLWSRLRRVFLLCVFLGMLGVPATGQGLEVVPVCDD
                     DYLPQLEQLIDSASHSLDIVAYEFLSEGGEIRRITRRLVDRLKVNPALRVR"
     gene            complement(341..1198)
                     /locus_tag="PKO06_17425"
     CDS             complement(341..1198)
                     /locus_tag="PKO06_17425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HNV71489.1"
                     /translation="MQEPLPFFTGRGPMLLGMVHVQALPGTPFARLSVPGIVARAVDE
                     ARQFKKEGFDGIILENMHDRPYLKGAVGPEITAAMTAVACRVREAVGPDFPIGIQILA
                     GANREALAVALAAGLNFIRAEGLVFAHVADEGWIEACAADLLRERKRLGADHIQIWTD
                     IQKKHSAHAVTADLSLTEWAHGAEFFGADAVIVTGTHTGAPADEGHLVEVRRATKLPV
                     AIGSGLTAQNAARFATAHAWIIGSSIKKGGKWENPLDPQALREMVRARDDLYRKAKAV
                     LPKPTRRSR"
     gene            1270..>2086
                     /locus_tag="PKO06_17430"
     CDS             1270..>2086
                     /locus_tag="PKO06_17430"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV71490.1"
                     /translation="MKRHFDPEIVVCGGGPAGGTAALYAARIGRRVTLLTGNPYGSLI
                     AANREIDNFPGTPGLDGVRFIEQLHRQCADAGVTLVQQHAVSLELENRVKILRTDAAR
                     TFETPVVIVAIGTRPLRPPIPGLDTFWGRGVSVCAWCDGALHRGRSVAVYGGGNTAYI
                     SVRLLSRLAKDVHLLLPKRAGTAFQAVQSQVAEATNVHVHQEVEVLALEGQTHLASVH
                     LKTAGQPLTLGIDGFFLALGQKPHTDFLRPLVDLDAAGYIKADSNGSTSCPGVY"
BASE COUNT          394 a          666 c          620 g          406 t
ORIGIN      
        1 cgcgcacccg cagagcagga ttcaccttga ggcgatcgac caaccggcgc gtgatgcgcc
       61 tgatctcgcc cccttcgctc agaaactcgt acgcaacgat gtcgagcgaa tgactggctg
      121 aatcgatcag ctgttcgagc tgcggcaggt agtcatcatc gcagaccggc accacttcga
      181 gaccctgtcc cgttgccgga acgcccagca taccaagaaa aacgcacagc agaaagaccc
      241 ggcgcaggcg cgaccacagg ttgccgacac ggaatctgtg ccgcatgaac ggcgcatacc
      301 gctcgcccgc gggtgaatgc aacacgtgcg gcggcgtcac tcagcgtgac cgtcgcgtcg
      361 gttttggcaa cacggccttt gcctttctgt acaagtcatc gcgagcccgc accatctctc
      421 gaagggcctg aggatcaagt ggattttccc atttgccgcc cttcttgatg ctcgagccga
      481 tgatccaggc gtgagcggtc gcgaagcggg cggcgttctg cgccgtgagc cccgagccga
      541 tcgccacagg gagcttcgtc gcccgtcgaa cctcgacgag atgcccttca tctgccggtg
      601 cgccggtgtg cgtgccggtg acgatcaccg cgtccgcgcc gaaaaactcg gctccgtgcg
      661 cccactccgt aaggctgaga tcggccgtca ccgcgtgcgc cgagtgcttt ttctgaatgt
      721 cggtccagat ctgaatgtga tcggcaccca gacgtttgcg ttcgcgcagc aggtcagcag
      781 cgcaggcttc gatccagcct tcatcggcga catgtgcaaa gaccaacccc tcagcgcgaa
      841 tgaaattcag tccggcagcc agcgcgaccg ccagcgcttc gcgattggct ccggccagaa
      901 tctggatgcc gatgggaaaa tccggcccga ccgcctcgcg cacccggcag gccacggcgg
      961 tcatcgcggc cgtgatttca gggccgaccg cccctttcag gtagggtcga tcatgcatgt
     1021 tctcgagaat gatgccgtcg aatccttctt tcttgaactg ccgggcttcg tccacggcac
     1081 gcgccacgat tcctggcacc gagagtcgcg caaaaggcgt tccggggaga gcctgcacat
     1141 ggaccatacc aagcagcatc ggaccgcgtc cggtaaaaaa cggcaagggt tcctgcatcg
     1201 ttctgttcct tcctttttca cggcagatgt cggcagtata cctcagaacc gcatggtata
     1261 atcacttcga tgaaacgaca ttttgacccg gagatcgtcg tgtgcggcgg cggtccggcg
     1321 ggcggtaccg cggctctcta tgcggcgcgc atcggccgac gcgtcacgct gctcaccgga
     1381 aacccgtacg gcagcctgat tgccgccaat cgcgagattg acaactttcc ggggaccccc
     1441 ggtctggacg gcgtacgctt catcgaacaa ctgcatcggc agtgcgccga tgcgggcgtg
     1501 acgctcgttc agcagcatgc ggtctctctg gaattggaaa atcgagtcaa gattctgcgc
     1561 accgatgctg cgcgcacctt cgagactccg gtggtcatcg tcgccatcgg cacccgaccc
     1621 ttgagaccgc cgatccccgg gctcgacacg ttctggggtc gaggtgtttc ggtctgcgcc
     1681 tggtgcgatg gtgcgttgca tcgcggtcgt tccgtcgccg tctacggtgg cggcaacacc
     1741 gcctatatca gcgtgcggtt gttgagtcgt ctggcgaaag acgtgcatct tctgttgccc
     1801 aaacgcgccg gcacggcttt tcaggcggta cagagccagg ttgctgaagc gaccaatgtg
     1861 cacgtgcacc aggaggtgga agtgctcgct ctcgaagggc aaacgcacct ggcttcggtt
     1921 cacctgaaaa cggcaggaca accacttacg ctcggcatcg acggtttttt tctcgccctc
     1981 ggtcagaaac cgcataccga ctttctgaga ccgctcgtcg acctggacgc tgccgggtat
     2041 atcaaggcag attccaacgg aagtacaagt tgtcctggcg tgtatg
//
LOCUS       DAORHD010000953         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67352_length_2085_cov_3.332512, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000953 DAORHD010000000
VERSION     DAORHD010000953.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67352_length_2085_cov_3.332512"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..1839
                     /locus_tag="PKO06_17435"
     CDS             328..1839
                     /locus_tag="PKO06_17435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV71491.1"
                     /translation="MSGRRFLPTSLFARLFLLVSLLSCVLVSIGGYGMYLQALSMIDR
                     EANQRLAAGASTLAAHLWPYINDPADANAVSLFRDVLGPTEGLGWIRTVYWVDMSASA
                     PAFLATLSFPDSAKKQHRPPSLDHLEDALDDGLDLLEAGRPYYPDPRFLHGNQQLKTI
                     FMPIMDHHGFLHSFIGLEADSSLLNLRASLRHTLLLVLGAAVLSALLASLLIAGSVSR
                     HADRLVKLLHHIEKREPVGEEMSGIHEFATLHRGLSALGQSIVDGDQRLHQMYEQKLN
                     ELFLMGGAIAHQLRNPLAAAELHLGLLRRNPSSSESLKELQLVLEQMNHLVTRFLGYS
                     KKVQPVPQLLVAYDWLSDWATRLRSSHPDISISVQAEKHATLFADPDLLQEILANLIR
                     NSIEAASAKVQIDLTATAIPGAARLCFQDHGPGIPTDLMPSLFAPFTSRKAQGTGLGL
                     ALVRKFVEAHHGRIEYQPGPSGGAGFLIDLPSPPSHSPSAFDEIRPVSTEKSP"
     gene            1836..>2085
                     /locus_tag="PKO06_17440"
     CDS             1836..>2085
                     /locus_tag="PKO06_17440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71492.1"
                     /translation="MNILIVDDNESLARGLVSYLKSERHDALMATAVRDGLHLVGTQK
                     FDLVITDLRLPDGSGLDIIKSCRSAEDPPEVILMTAFSS"
BASE COUNT          379 a          662 c          513 g          531 t
ORIGIN      
        1 tcttctcgcg cagacttcca agaccttctt cagcggccgc attcagacct atgtgagggg
       61 tgggttcggc agacaagccg tagtggttct cattgcctca aacctctccg gcgaagagat
      121 cgtttctcaa acctggtatc agtttttcga gcctctttct ctatgagttc tgcgacaagt
      181 tttccttgga gtccaccact ttttccttcc tgagttttct gttgttttca tccaagttca
      241 gtcgaagtct tttgtttcgg gcgcgttctt cgcttgcgat gactcttttc atcgttgtcg
      301 actcggactg acgtggcttc gctttttatg tcggggcgac gtttcctgcc caccagtctg
      361 tttgcgcgct tgtttctatt ggtttccctg ttgagttgtg tcctggtgag catcggcggc
      421 tacggcatgt accttcaggc cctcagcatg atcgatcgcg aggccaatca gcggctagca
      481 gccggagctt ccaccctggc ggcgcatctc tggccgtaca tcaatgaccc agccgacgcc
      541 aacgccgtgt cgctgtttcg tgatgtttta ggtcctaccg aaggtctcgg ctggatcaga
      601 accgtttact gggttgatat gtccgcttcc gccccggcgt tcctcgccac cctttcgttt
      661 cctgactccg cgaaaaagca gcatcgcccc ccttcgctcg atcacctgga agacgctctc
      721 gatgatggtc tcgacttgct cgaagccggc cgaccatact accctgatcc ccgttttctg
      781 cacggtaacc agcaactcaa gaccatcttc atgcccatca tggaccatca tgggtttctg
      841 cattcgttca tcggcctcga agccgacagc tccctgctca accttcgcgc ctctcttcga
      901 cataccctgc tgctggtcct cggagccgcc gtcctgtcgg ccttgctggc atcgcttctg
      961 atcgccggca gcgtctcacg tcatgccgac cgtctggtca aacttctgca tcacatcgag
     1021 aaacgcgaac cggttggtga ggaaatgtcg gggattcacg agtttgccac gctgcatcgt
     1081 ggtctctcgg cactcggaca atcgatcgtc gatggcgacc aacgactgca tcaaatgtat
     1141 gagcagaaac tgaacgaact gtttctgatg ggtggtgcga tcgcgcatca gctccgtaac
     1201 cccctcgccg ccgccgaact ccatttgggt ttactccgtc gcaacccgtc ttcttctgaa
     1261 tctctgaagg aattgcaact cgtacttgag cagatgaatc acctggtcac tcggtttctg
     1321 ggctactcca aaaaagtcca gcctgttcct cagttgctcg ttgcttacga ctggctgagt
     1381 gattgggcca cgcgtctgcg atcgagccac ccggacattt ccatctctgt ccaggcagag
     1441 aaacacgcca ctcttttcgc tgaccctgat ctcttgcagg agattctggc gaacctgatc
     1501 agaaacagta ttgaagcagc ctccgcgaag gttcagatcg atctcaccgc cacggcgatt
     1561 cccggcgcag cccggctctg ctttcaagat catggtcccg gcattcccac cgacttgatg
     1621 cccagccttt ttgctccgtt cacctcccgc aaggcccagg gaaccggtct gggcttagct
     1681 ctggttcgca agtttgtcga agcccatcac ggaagaatcg agtatcagcc cggtccgtca
     1741 ggaggtgccg gtttcctcat cgatcttccc tctcctccct cgcattcacc atccgctttc
     1801 gacgaaatcc gtccggtatc aacggagaaa tccccatgaa cattctgatc gtcgacgaca
     1861 acgaatctct ggcccgtgga ctggtctcct atctgaagag cgaacggcac gacgctctca
     1921 tggcgacagc cgtgcgcgac ggtctgcacc ttgtgggcac acagaagttc gatctggtca
     1981 tcaccgatct gcggctcccc gacggctcag gtctcgacat catcaagtcc tgccgcagcg
     2041 ccgaggatcc gcccgaagtg atcctcatga cggccttcag ctccg
//
LOCUS       DAORHD010000954         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67451_length_2083_cov_4.323471, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000954 DAORHD010000000
VERSION     DAORHD010000954.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67451_length_2083_cov_4.323471"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(648..1565)
                     /locus_tag="PKO06_17445"
     CDS             complement(648..1565)
                     /locus_tag="PKO06_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNV71493.1"
                     /translation="MAEKVYSFLNDFVFKYAFGREKNERLLVCLLNALLRLEGQSRIQ
                     EVKLLNPFNLKEFADTKLSIVDVKAVDGYRRRYNVEVQVNAQSAFIPRSLYYLGKLFT
                     EQLGEGEPFSNLFRATSISILEFTLFPHSPDIQSIYRFRNLVTNEDLTDLLEAHFIEL
                     PKFTQEKPRQLRTPFERWLYILKFGSRYDAKTALPRELQQEEGVDMAIAELQKLNSDR
                     EMRQLIEAREKARHDEATRFAEAELRGKLSKMREIAEKLLAEGMPIAKVADLTGLAEA
                     EVRTLGAERSRHSRVQEPPQPHSHTKRRT"
     gene            1837..>2083
                     /locus_tag="PKO06_17450"
     CDS             1837..>2083
                     /locus_tag="PKO06_17450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71494.1"
                     /translation="MPQAKRKPVASKKFTYADYCTWPEEVRCELIDGVVFDAGQGRPS
                     VGPAPGTARFGRTMTPAPSRLHSSISVNLTTEMSTFFR"
BASE COUNT          446 a          597 c          538 g          502 t
ORIGIN      
        1 ctcgacgacg gttttcgata acatgctctg cgcctcctcg aggtcagtga cctcacttct
       61 tatagctcgc gtcgtggctt ttcttcaccc tcattcgtgg tcaagatcgc cctgtttacg
      121 gggcttgagt tcgacctgaa cagaatcttt ccaccccaac cgaaggtcgt gcgcgaagca
      181 aagccgtacc cgtaccgcac gtaacgcgcg caacttgaac ttcttccagg ttttccgcgg
      241 gttccgaggg cagacgctga ccgatcggtg gagacgaggg agcagatcga gtgtcgatca
      301 tacttcctgc aaggcactca gatcattctc cggcagctcc gtaacgactg ccaccagatg
      361 aatgggccaa cctcgagcaa gcaggtctcg tgcaatcgag agcatcgctt ttcgaagccc
      421 ttcttcgtca gagtcatcat gccagctctg gatgccgatt tttaggccaa gcctgtaacc
      481 ctcgagatat ccggcattcc aacgagcatg agtttccgct cgacgggtgg cgacttcgta
      541 tcgttctttc tctctcgctt cgtcaagctg ctttctttcc tggtcagatt gcacggttca
      601 ccattctcca tctagtcgcg acgatttgag aactcgctcc atgagcctta cgttcgtcgt
      661 ttcgtgtggc tgtgaggctg cgggggttcc tgaactcgcg aatgccgtga tcgttctgca
      721 cccaacgttc gcacctcggc ttccgccagc ccagtcagat cggcgacctt agcaatgggc
      781 attccctctg ccagaagttt ttctgcaatc tcacgcatct tcgacagttt cccgcgcaac
      841 tctgcttcgg cgaaacgcgt ggcttcgtca tggcgggctt tttcgcgcgc ctcgatgagc
      901 tggcgcatct cccgatccga attcagtttc tgcaactcag cgatagccat gtcgacaccc
      961 tcctcttgct gtagttcgcg cggtaaggcc gttttcgcat cataacgact gccgaacttc
     1021 agaatgtaca accaccgctc aaacggcgtc cgcaactggc gaggcttttc ctgagtgaat
     1081 ttgggcagtt caatgaagtg tgcctcgaga agatcggtca agtcctcgtt cgtcacgaga
     1141 ttccgaaatc gatagatgct ctgaatatca ggcgaatggg ggaacagcgt aaactcgaga
     1201 atcgagatgc tggtcgcccg gaaaagattg gaaaacggct ctccttcgcc gagttgttcg
     1261 gtgaacagct tgccaagata gtagagcgaa cgcggaatga acgccgactg agcgttcacc
     1321 tgcacctcga cattgtaccg gcgacgatat ccatccacgg ctttgacatc gacgattgag
     1381 agtttggtat ctgcaaattc cttgagattg aaagggttca ggagctttac ctcctgaatg
     1441 cgactctgcc cttcgagcct cagcagtgca ttcagaagac aaaccagcag acgctcgttc
     1501 ttctcgcgcc cgaacgcata tttgaagaca aagtcattga gaaacgaata cactttttcc
     1561 gccatgaaca acctccggat ccattctatc ataagagcaa gcctgagatt gacagccaag
     1621 tcccagagac tcgaggtgat cggagtgatc ggatcggaga tcggggggat cgggtgacag
     1681 catacccaat tcagcagacc cgcttcacgc ctgaaggaag tgcggtgacc gaattccccg
     1741 ccccatgagc cgttgttcct ttcccatggc tcctgacagt ctttatcttg tgttagaatg
     1801 cttgaacgta ttactctcat ggtcggagaa acaggtatgc cacaggcgaa acgaaaacct
     1861 gtcgcgtcga aaaagtttac ctatgccgat tactgcacct ggccggaaga ggtgcggtgt
     1921 gaattgatcg acggggtcgt gtttgacgct ggccagggac ggcctagtgt cgggccggcg
     1981 ccagggacgg cgcgtttcgg tcggaccatg accccagccc cgtctcgtct tcattcgtcc
     2041 atctctgtca atctcaccac ggagatgagc accttctttc gcg
//
LOCUS       DAORHD010000955         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67578_length_2081_cov_2.959033, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000955 DAORHD010000000
VERSION     DAORHD010000955.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67578_length_2081_cov_2.959033"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="PKO06_17455"
     CDS             complement(<1..204)
                     /locus_tag="PKO06_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71495.1"
                     /translation="MANEITEVRKEVREIQELVRRGMSKHEVAFEMVKLINGGRNYPT
                     FEERRTMLLQLYRDVFLMMTEVEK"
     gene            326..967
                     /locus_tag="PKO06_17460"
     CDS             326..967
                     /locus_tag="PKO06_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71496.1"
                     /translation="MHTKQCRRGFTLMEIIIASIVMVGFFVGVYKVYSAVMKSAKKTN
                     WSLSAQQWARNGLTLIREEMQRASDKIEFDGIGKSTITPMPFKINATSPVPDGGKIAE
                     WYICIPFKNVEGTEPGAVLKSTLTLQGGKVTYERKKESGDNSHDPDITRVIMENVGSF
                     SIDIEPLDVDFSEKGKLVKLGVELKHPSTTEFPDTRVIEETAAKVEVEVTKGL"
     gene            969..1535
                     /locus_tag="PKO06_17465"
     CDS             969..1535
                     /locus_tag="PKO06_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71497.1"
                     /translation="MRIERVRAGITFVELCLAVIILAMALLPIFGLMSASNTATRQQK
                     AEGVAANLAKEEMNRLLYVITRTNCETMQSAEIENRDVEGNVFRITKQFFFYDNTAFN
                     VTYPEVQWHDMRQCTGGIETNMLNTATHIKTREKNLLEVTQESQMMIADVVLKIEWRL
                     PNEADFKPTSFLNLVARRAFLVEKKATP"
     gene            1532..>2081
                     /locus_tag="PKO06_17470"
     CDS             1532..>2081
                     /locus_tag="PKO06_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71498.1"
                     /translation="MIKQRKGMAIAVVLLFVTCTLAFAGAMFYFRKEVKQQNVINFDF
                     LQANFLAQAAIQHVLLKAHILPQEAYDSGVIMRGFCPLQAVISGTTANAGTQKTPYPM
                     QIFYSDCNTASRPWILSFSGVTPSDYEYRVASMVVIGAFTDTDKLEMIQTIQITAHGT
                     IRNLRGGRGDRTEVMTKTLELKR"
BASE COUNT          529 a          541 c          546 g          465 t
ORIGIN      
        1 cttttccacc tcggtcatca tcaggaacac atcgcgatac agctgcagca gcatcgtgcg
       61 gcgttcctcg aacgtcgggt agttgcgccc gccgttgatc agcttgacca tctcgaaagc
      121 cacctcgtgt tttgacatgc cccggcgaac caattcctga atctcgcgca cttcttttcg
      181 aacttcggtg atctcgttgg ccattcttcc ctccagcagt gtcgaacaaa ctctgcgcat
      241 cttttgaacg cgatggatct ttccgtccca aatgtaacat atttgattct caggtggtat
      301 aatagttatg gggagtggaa aggccatgca cacaaaacag tgccgacgcg gtttcacgct
      361 gatggaaatc atcatcgctt cgattgtgat ggtcggtttc ttcgtcggcg tgtacaaggt
      421 ctattcagcg gtcatgaagt cggcaaagaa aaccaactgg tcgctctcgg cgcagcaatg
      481 ggcacgaaac ggtctgacgc tgatacgaga agagatgcag cgggcgtcag ataaaatcga
      541 gtttgatggg atcgggaaaa gtaccattac cccaatgcct ttcaagatca atgcaacctc
      601 gccagtaccc gacggaggca agattgccga gtggtacatc tgcattccat tcaagaacgt
      661 cgaaggaacc gaacccgggg cggtcctcaa gtccacgctt acgcttcagg gtggcaaggt
      721 cacgtatgag cgcaagaaag agtcggggga caactcgcac gaccccgata tcacgcgcgt
      781 tatcatggaa aacgttggtt cgttctcaat tgacatcgaa cctctcgatg ttgatttctc
      841 ggaaaaggga aagcttgtga agcttggagt ggaattgaaa caccctagta ccacggagtt
      901 tccggatacc cgcgtcatcg aagaaacggc agccaaggtc gaagtcgagg ttaccaaagg
      961 gctgtgatat gcgaatagaa agggttcgag ctggtatcac gttcgtcgag ttgtgtctgg
     1021 ccgtcattat tctggccatg gcactgctgc ctatcttcgg gctgatgtct gcctcgaaca
     1081 ccgcgacccg ccagcagaag gcggaagggg ttgccgccaa tctggccaag gaagaaatga
     1141 atcgcctgtt gtatgtcatc acgcgtacaa actgtgagac gatgcaatcg gctgagattg
     1201 agaaccgcga tgtcgaagga aacgtgtttc gcatcaccaa acagtttttc ttctacgaca
     1261 acacggcatt caatgtgacc taccctgaag tccagtggca cgacatgcgg caatgtaccg
     1321 gtggtattga aacaaacatg ttgaacacag caacccacat caagacacgc gaaaagaacc
     1381 tccttgaggt cacccaggaa tcacagatga tgatcgctga tgtcgtgctg aagatcgaat
     1441 ggcgtttgcc gaatgaagcg gatttcaagc cgacgagttt cctgaatctg gttgcccggc
     1501 gggcattcct ggttgaaaag aaggcaaccc catgatcaag caaagaaaag ggatggcgat
     1561 tgcagtcgtg ttgctcttcg ttacctgcac cctggcgttc gccggggcca tgttttactt
     1621 tcgcaaagag gtcaaacaac agaacgtcat caacttcgat tttctgcagg ccaacttcct
     1681 ggcgcaggca gctattcagc acgttctgct caaagcacac attctgccac aagaggccta
     1741 tgattccggc gtcatcatgc gtggcttctg tccgctgcag gccgttatct caggaaccac
     1801 agcaaatgcc ggaacacaga agactccgta tcccatgcag atcttctaca gcgactgtaa
     1861 cacggcaagt cgcccatgga tcctcagctt ctcaggcgtc accccaagcg attatgagta
     1921 tcgagtggca agcatggtcg ttatcggggc gtttacggat accgacaaac tcgaaatgat
     1981 ccagacgatt cagattaccg cccacggaac tattcggaat ctccgtggtg ggcgtgggga
     2041 ccggaccgag gtaatgacca agacgcttga gctgaaacgg a
//
LOCUS       DAORHD010000956         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67599_length_2080_cov_5.009383, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000956 DAORHD010000000
VERSION     DAORHD010000956.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67599_length_2080_cov_5.009383"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..1619)
                     /locus_tag="PKO06_17475"
     CDS             complement(198..1619)
                     /locus_tag="PKO06_17475"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71499.1"
                     /translation="MRKVLYVVLGVWCLAVAPELSAAGKKPATAKTAVATATAKTQLG
                     EEYKEVHAFSRMLGFDPHPMLTEYLQDSPKNILGRPMTFTVETEETWKAEPVSLQEIG
                     FQVKVLSQGQLVQTLRVPRKALKTRDAKPGALLGEVSDGDLAVRFLVAEAQVEGGRLV
                     ELTYDGRLLAREAGVSTSSPVPVTSEVGQPPEPAKVPVLPPVVATPPVVTETSGVLAP
                     APESPETPVGLTTGTDIPSVVTRLLEKAYSLPPEQEKVRLSLLRKALAMLGDVQQPGP
                     EVERLVKDLQNRTAGVDAVPANVPASGSAQIELAPVTASTDGVSDEARTLYDQAVRAF
                     ALGQEPEARDCLRRAVEKAPGYHQAWVLLGKNALANSKYARAQEAFQQAVSLRGDDVE
                     SGVGFFKACYYLGDAEKGLELLKGLVSRNPQALLAREALAEAYYQAGDLESCLIECRQ
                     ILDRDPKNTRIQDLFARTSQRMK"
     gene            complement(1638..>2080)
                     /locus_tag="PKO06_17480"
     CDS             complement(1638..>2080)
                     /locus_tag="PKO06_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71500.1"
                     /translation="EKHRWRDLETLRGEYEKLCELLREQQQGAYLEQTERLVLELCRA
                     RDRAEKIRQLFQEKAAAPGLNTPGDERMMASLSNLRSYRDRYEEQKRETLALFQTLRI
                     KALDSAADLGDLDRLVARVEGIRYLAEHIDGEEPVPLRRPGVTE"
BASE COUNT          401 a          703 c          551 g          425 t
ORIGIN      
        1 ggcggcgcat acagagcctt gtacaaagcc ccgatgacgg cacagactcc cagaaacgaa
       61 atcagggcgg caaaacgagt aatcattgtc gtcctccgag ttcagaaacg tgttttcgcc
      121 gtccgcctgc ggccacggac gggtggttcc atgatgcggc gccaacgccc acctgtcaag
      181 aatttccgcg cggcaggcta cttcatccgc tgactggtgc gggcaaacag atcttgaatc
      241 cgagtatttt tcgggtcacg gtccagaatc tgtcgacact cgatcagaca ggattcgaga
      301 tcacccgcct gatagtaggc ttccgccaga gcttctcgcg cgagcaacgc ctgcgggttc
      361 cgggaaacca agcccttgag cagctcgagg cccttttccg catcgcccag ataatagcag
      421 gccttgaaga aacccacgcc gctctcgaca tcatccccgc gcagcgacac cgcctgttga
      481 aacgcctcct gggctcgcgc atacttggaa ttcgccaggg cattcttccc cagcagcacc
      541 catgcctgat gatacccagg cgctttctcg accgcccgac gcagacagtc gcgcgcctcc
      601 ggctcctgac caagcgcaaa ggccctcacg gcctgatcat acagagttcg cgcctcatct
      661 gacactccgt cggttgacgc ggtgactggc gctaattcaa tctgagccga cccagaagct
      721 gggacattcg cgggcacggc atcgactccc gccgtgcgat tctgcaggtc tttgaccagc
      781 cgttcgacct cggggcccgg ttgctgcacg tctcccagca tggcgagtgc tttccgcaac
      841 agactcagcc gaactttttc ctgttccggg gggagggagt aggccttctc cagaaggcgg
      901 gtgaccaccg agggaatgtc ggttcccgtc gtcagcccca ccggggtttc cggggattcg
      961 ggcgcgggcg ccaggacgcc cgacgtttct gtcaccaccg gcggcgttgc cacgactgga
     1021 ggtagtaccg gcactttcgc cggttcgggc ggttgcccaa cctcagatgt gaccgggaca
     1081 ggagacgaag tggagactcc ggcctcgcgc gccagcaatc gaccgtcgta cgtcagttcg
     1141 accaggcgcc ccccctccac ctgcgcctcc gcgaccagaa atcgtacggc cagatctccg
     1201 tcactcacct cgccaagcag cgcaccaggc ttcgcgtcac gcgttttcag cgcctttctg
     1261 ggaacacgca gcgtttgtac aagttgcccc tggctcagca ccttcacctg aaagccgatc
     1321 tcctgcagtg acaccggctc tgccttccag gtttcttcag tttccaccgt aaaggtcatc
     1381 ggtcttccca gaatgttctt gggcgaatcc tgcagatact cggtcaacat cgggtgtgga
     1441 tcaaagccca gcatgcgact gaacgcatgc acttctttgt actcttcacc caactgcgtc
     1501 ttcgcggtcg ctgtggcgac agccgtcttc gcggtcgcag gcttctttcc ggccgcggac
     1561 aactcgggcg caaccgccag acaccagact cccaaaacga cgtatagaac ctttctcatg
     1621 gccttcctcc tcacgattta ctcggtgact ccgggccgtc gcagcggcac cggttcttca
     1681 ccgtcgatat gctcagccag atatctgatg ccctcgacac gcgccaccag tcgatccaga
     1741 tcacccagat cagcggccga atcgagagcc ttgatgcgca gcgtctgaaa caaggcaagc
     1801 gtttcacgct tctgttcttc gtagcgatca cggtacgagc gcagattgct cagacttgcc
     1861 atcatgcgtt catctcccgg ggtgttcaat ccaggagccg ccgctttttc ctgaaacagc
     1921 tgccgaatct tctcggcacg atcgcgcgcg cgacacaact ccagaaccag acgttccgtc
     1981 tgttcgaggt acgctccctg ttgctgttcg cgcaacagtt cgcagagttt ctcatactct
     2041 ccgcgcaacg tctccagatc acgccaccga tgcttttccg
//
LOCUS       DAORHD010000957         2080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67611_length_2080_cov_3.564444, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000957 DAORHD010000000
VERSION     DAORHD010000957.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67611_length_2080_cov_3.564444"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..1741)
                     /locus_tag="PKO06_17485"
     CDS             complement(356..1741)
                     /locus_tag="PKO06_17485"
                     /inference="COORDINATES: protein motif:HMM:NF024678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNV71501.1"
                     /translation="MTRKLDIALISPRTGLYRFGTGVFPVTLRYAPLNMVTLAAMIPS
                     EMRGNITIYDESVQKVDPAKIKADIVGLTGITGVSLRSYAYAEYFRRQGVTTVMGGPH
                     ATLMPEEAAKHVDAVVVGHGYETWPRLLRDYQAGRLEKFYYPPEHINWSTIPDPHRGM
                     FANKRFITTNSTQAVFGCPHHCEFCVTPIICKGKYERRPIADVVNDVARMPGRYVTFL
                     DPSPVENVEYSIELYKALAPLKKRWGGLATTRIVDQPALMNAMEASGCFGLLIGFESL
                     TDSGNTTIGKGFNDISKYRTLMRELHARGISVMGCFVHGLDSDKPDCFKHTLDFIHDA
                     HVDLPRFTVCTPFPGTPFFNRLKGEGRILTENWTLYDAQHVVFKPQNMSVDELQEGHI
                     KCWESAYRISSIFKRLAGSRTFLEYTILANIGYRFYCHGHRRFPPHRMAHDWKLRVTT
                     PEDIAKIQPVC"
BASE COUNT          447 a          602 c          583 g          448 t
ORIGIN      
        1 gaacgattca ttcccgcccc tgcatctgcg accgccacac ctggtgcagc cgtgagaacc
       61 atgaagaaac acattccgaa aacggttttc caaacccttg acgccatctc gcatcgcaca
      121 ctgcgaaacc accatttcat gaaagatcag cctcgattgt atttgactcc gaaatagagc
      181 actttcactc cttcgaccga tctctcacga tcatgtactg atttctcgtg aacaaatgta
      241 aagcaatgtc atgacgcgag catggaatag tctgacgcaa atacgcccaa tgcccgtccc
      301 gtgcggaaaa aaagcgtttg tgcgcaagga tgcccgcgac gaacggcccc agacgtcaac
      361 agactggctg gattttcgcg atgtcttcgg gggtggtgac gcgcagtttc cagtcgtgag
      421 ccatgcgatg cggcgggaac cggcggtgac cgtggcagta gaagcggtag ccgatattgg
      481 cgagaatcgt gtattcgagg aaggtgcgcg agccagccag tcgcttgaag atgctcgaga
      541 tgcgatacgc gctttcccag catttaatat ggccttcctg gagttcgtcg acgctcatgt
      601 tctggggttt gaacacgaca tgctgcgcgt cgtagagggt ccagttttcg gtgagaatgc
      661 gcccttcgcc cttgagtcga ttgaagaacg gcgtgccggg aaacggcgtg cagacggtga
      721 aacgcggcag gtcgacatgc gcgtcgtgaa tgaagtcgag cgtgtgtttg aagcagtcgg
      781 gcttgtcgct atcgagacca tgcacgaaac agcccatgac cgagatgccg cgggcatgca
      841 gttcgcgcat cagcgtgcga tacttgctga tgtcgttgaa tcctttgccg atcgtcgtat
      901 tgccgctgtc ggtcagcgac tcgaagccga tgagcaggcc gaaacagccg ctggcttcca
      961 tggcattcat cagagcgggt tgatcaacga ttcgcgttgt cgctaatcct ccccatcgct
     1021 tcttcaaagg cgccagcgcc ttgtacaatt caatcgagta ttcaacattc tcaacggggc
     1081 tcgggtcaag aaacgtgaca tatctgcccg gcatgcgcgc cacatcattc acaacgtctg
     1141 cgatcgggcg ccgctcgtac tttcccttgc agatgatcgg cgtgacacag aactcgcagt
     1201 gatgcgggca cccgaagacc gcctgggtac tgttggtcgt gatgaatcgc ttgttcgcga
     1261 acatgcctcg gtgcgggtcg ggaatcgtcg accagttaat gtgttcaggt gggtagtaga
     1321 acttctcgag ccgacccgcc tggtaatccc gcaacaaacg cggccaggtt tcatagccat
     1381 gcccgaccac gacggcgtcg acgtgcttcg ccgcttcttc gggcatcagc gtggcgtgcg
     1441 ggccgcccat gaccgtcgtc accccctgtc gccggaagta ttcggcatag gcatacgaac
     1501 gcaatgacac gccggtgatg ccggtcagac cgacgatgtc ggccttgatc ttcgccggat
     1561 ccaccttctg aacactttca tcatagatgg tgatgttccc gcgcatctca gacggaatca
     1621 tcgccgccag ggtcaccatg ttcagaggcg cataccgcaa ggtcaccgga aagacaccgg
     1681 tgccgaaccg gtacaacccc gtacggggcg aaatcaaggc aatatcaagt tttctggtca
     1741 tggcttctcg ttccttcgcg aaccaggcat ttcggccatt ctagtttttt ccgcccccga
     1801 aagcaaatca gaaccgtttc ccatgagaga cactcccggt ccagcaacga attctgaccg
     1861 cacacttctt cttggcacct gccgcaagcg cgtcgagata gtcagcctgc tgacgactca
     1921 ctgcaatgcg aagccgcatc tgatcggggg ttatcccggc agttgtgaga aggcgtgctg
     1981 ccaggcgcgc tcctgccaga ggcgggcttc ttcccattcg ggggttcggc gccagccgga
     2041 gtgtacaagt cgcacttcgg tatgatcttg atcaacgggg
//
LOCUS       DAORHD010000958         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67829_length_2076_cov_4.355764, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000958 DAORHD010000000
VERSION     DAORHD010000958.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67829_length_2076_cov_4.355764"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="PKO06_17490"
     CDS             complement(<1..2029)
                     /locus_tag="PKO06_17490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71502.1"
                     /translation="MSRERRENGDRLDRVPWPGFARVSNDLMMTADGWVATEFAVPEI
                     GTESVLLARRVSEPRLSWLLPGICVWSLFWLVGGLLLWWRSNWWFQLPGGMKLQLILA
                     FGLALSPALLLSLTNIERRWTGHAVRARDVREEGLERRTRQVEESYLLTEEACGRSLR
                     DIFTLPETGRWLKMIRKARGRERLERMSELMARFGELSRRIGWFCEVQRLISADGQAA
                     SGFEHRGAERRIEGREALLLFVGRTLLKQWRTDDLEGTTDKASQVLIEANAEDALTMF
                     RGFFPPDDAPGMLLLPYSGIRPWNQGESALMMIVTDKERAVAWLFVSLLNQALWRLHF
                     QQWSEVLAADSWHVTGIPSPVPMLRLADPVASVYVSDSRGERLTMQPVFLGQSSTEAW
                     AALLARETGEGIMVEPPEHRCAETVFVKPAQLLEGLLLRVCESSPTGAADELSARTVD
                     LLAPFAILAAGLVLSVLVARTYLRPLLRLQQAAREVIRERFETRLVIEGGNEFAELAR
                     AFNLMAAGIMEGRELRRYVSLSVQQVAGDVLAEHSGVARATEAVILFGAVQGFRLRAE
                     SLDADTIMPRLRDHLAAWAGAVRQAGGEVDKFIGDRILAVFFPDRCGGWNAAVVAACT
                     AVQRMITVPTDPALGDWSGIGVAAGPVLSGILGTPEVRLEYTVVGDTVNTRQ"
BASE COUNT          466 a          707 c          564 g          339 t
ORIGIN      
        1 gctgtctggt attcaccgtg tcgccaacta ccgtgtattc cagccgcact tcgggggttc
       61 cgagaatacc agacagcacc ggtccggcag caacaccgat tccggaccag tcacccagag
      121 cagggtcggt gggaacggtg atcatgcgct gaacggcagt gcacgcagcc acgaccgcgg
      181 cgttccagcc cccgcaacgg tcgggaaaga agaccgccag aatgcgatcg ccgatgaact
      241 tgtcgacttc gccgcccgcc tggcgcacag ccccggccca ggctgccaga tgatctcgca
      301 gtcgcggcat gatcgtgtcc gcatcgagtg actcggcccg caggcgaaat ccctggacgg
      361 ctccgaagag aatcaccgcc tcggtcgcgc gtgccacgcc cgaatgttcg gcgagcacat
      421 cgcccgcgac ctgctgcacc gaaagcgaca cgtagcgacg cagttcacgc ccttccatga
      481 tacctgcagc catgagattg aaggctctcg ccagctcggc gaattcattg ccgccttcga
      541 tgaccagtcg agtctcgaac cgttcgcgaa taacctcacg ggcggcctgc tgcaagcgca
      601 acagaggccg tagataggtc cgagccacca ggacggaaag gaccaggccg gcggccagaa
      661 tcgcaaaggg cgccagaaga tcgaccgtac gagcgctcag ctcatcagca gcacccgtcg
      721 gcgaagattc gcacacccgt aacaggagac cctcgagcag ctgggccggt ttcacgaaca
      781 cggtttcggc acagcgatgt tccggcggct cgaccatgat gccctcacct gtttctctcg
      841 ccagcagcgc ggcccacgct tcggttgaag attgcccgag aaagaccggc tgcatggtga
      901 gcctctcacc gcgtgaatcg ctgacataca ccgacgccac cgggtcggcc agacgcagca
      961 tcggcaccgg tgacggaatt ccggtcacgt gccacgaatc cgcggccagc acctcggacc
     1021 actgttgaaa gtggagtcgc cagagcgcct gattcagcag ggagacgaag agccaggcga
     1081 ccgcccgctc tttgtcagtg acgatcatca tcaaagcaga ttcaccctgg ttccagggcc
     1141 gaatccccga gtagggtaac aacagcatac ccggagcatc atcgggagga aagaagccac
     1201 ggaacatcgt cagcgcatct tcggcattcg cttcaatcaa gacctgcgat gccttgtccg
     1261 tggttccttc gagatcatcc gtgcgccact gtttcagcaa cgtgcgcccg acaaagagaa
     1321 gcaacgcttc acggccttcg atgcgccgtt cggcaccacg atgctcaaag ccactggccg
     1381 cctgaccgtc agccgagatc agacgctgca cctcgcagaa ccagccgata cgccggctca
     1441 actcaccaaa tcgggccatg agttcgctca tgcgctcgag acgttctcgc cctctggctt
     1501 tgcgaatcat cttcagccac cgcccggttt ccggaagcgt gaagatgtcg cgcagactcc
     1561 tgccgcaagc ctcttcggtc aacagataac tttcttctac ctgccgcgtt cttcgttcga
     1621 gaccttcctc acgcacatct ctggctcgga cagcatgtcc ggtccatcgt cgttcgatgt
     1681 tggtcaaact gagcagcagg gcaggcgaca acgccagacc aaaggcgaga atcaactgca
     1741 acttcatgcc cccgggaagc tgaaaccacc agttcgaccg ccaccacaac aatagtcccc
     1801 cgaccagcca gaagagactc cagacacaga ttcccggtag aagccacgag agccgcggct
     1861 cagatacccg ccgagcgaga agaactgact ccgtaccgat ttcaggaacc gcaaactccg
     1921 tcgccaccca gccatcagcg gtcatcatca aatcattgct gacacgcgcg aacccgggcc
     1981 agggcacccg atcgagtcgg tccccgttct cgcgacgctc ccgagacacg tcggcacttt
     2041 cagaaccaat cttcagatgc accccgacag aggcga
//
LOCUS       DAORHD010000959         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67898_length_2075_cov_3.598020, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000959 DAORHD010000000
VERSION     DAORHD010000959.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67898_length_2075_cov_3.598020"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PKO06_17495"
     CDS             complement(<1..350)
                     /locus_tag="PKO06_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71503.1"
                     /translation="MRLKLFLTTALFSCLALQPGFTVPKCYPPELPNHPDGYVFDYEP
                     SDPANNPDSRNPDNSDPANPECPYEADDYTANPDHPSGYNGLPRFPEQGGGNPQPEPE
                     QPPSEGNSSTVPGR"
     gene            complement(567..>2075)
                     /locus_tag="PKO06_17500"
     CDS             complement(567..>2075)
                     /locus_tag="PKO06_17500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNV71504.1"
                     /translation="RGLACLSTLVRQIRERHPRALLLDNGDTLFGSALTHYHQRFTPD
                     TPNPVIQAMNLMGYDALTIGNHEFDADLAVLASAAAQASFPFLAANIRATGKRFFRPW
                     VMFERQGVHIAVVGVTTPGIPHWADPESYAGLTFDDPIVPARLAVNEARKAGADVIIL
                     TAHLGSGVNSRTGQRIRESSWEGNIGFRLVREVAGIDALILGHTHELLATSVAGIPVL
                     QPKALGTHLARVDLHLEHRDGFWQMRDRQGSLVPVASETRPDFPIQASAQVLSNLTRR
                     YLDTPLATSPRHVAAMGCAFVDNPVTDLILQAMLEASGAEVALTALPPGSAQIPSGTV
                     RIRDVHRLYGFDNSIVCASLTGAQLKNILEHSSRFFHGWASQTSQLQLINSEVSFFNS
                     DLAAGIEYTIDLRQPIGTRITHLARSGKPLDPKRLVRVAVSNYRFNGGGEYPSFAAGI
                     DVERLDLEIRSAIIRHLMNRRLTLEADKNWRLLPDEIHELPRTPPESRQGPR"
BASE COUNT          410 a          565 c          699 g          401 t
ORIGIN      
        1 tctcgaccgg ggacagtcga actgttcccc tcgctggggg gctgctctgg ctcaggttgc
       61 gggttgccgc cgccctgctc cggaaaacgt ggcagaccgt tatagcctga aggatgatca
      121 ggattggcag tgtaatcatc agcttcatac ggacactcgg ggtttgccgg atcgctgttg
      181 tcaggatttc tgctgtctgg attattggcg ggatccgagg gttcgtagtc gaaaacgtaa
      241 ccatcaggat ggtttggaag ctccggtgga taacactttg ggacagtgaa cccgggttgc
      301 agggcaagac acgagaaaag agccgtcgtc aaaaagagtt tcagacgcac gcaaaatacc
      361 tccgcaggtg ttgaataact tggtttgaag gtcctctggg ccagatccgt ggccagctgt
      421 aagcgttgca cgatagcagg caggaaggag gatgtcaaga gccgcaggag ttggaacgtc
      481 gccgcgaaac cgcctgctga tgcagggtgt gcggtctcgg cgatgttgga aacgctcacg
      541 ccggcgaggt cgcgccggga tggtcgtcag cgcggcccct ggcgtgactc tggcggggtg
      601 cggggaagct catggatctc gtcggggagc agtcgccagt ttttgtccgc ttcgagcgtg
      661 agacggcgat tcatcagatg gcgaatgatc gccgagcgga tctcgagatc gagtctctcg
      721 acatcgatgc cggcagcgaa ggacggatac tcgccgccgc cgttgaaacg gtagttgctc
      781 accgcgactc gtaccagtcg cttgggatcg agaggctttc ctgatcgtgc gagatgggta
      841 atgcgtgttc cgatgggctg acgcaggtcg atcgtgtact cgatcccggc ggccagatcg
      901 ctgttgaaaa acgacacctc agaattgatc agctgcagtt gtgacgtctg gctggcccag
      961 ccatgaaaga aacgcgacga gtgctcgagt atgtttttga gttgtgctcc cgtcagtgac
     1021 gcgcagacga tcgagttgtc gaatccgtac aggcgatgca cgtcgcgaat ccgcacggtt
     1081 cctgaaggaa tctgcgcgct gccgggggga agcgccgtaa gcgcgacttc tgccccggag
     1141 gcttcgagca tggcctgcag gatcagatcg gttacgggat tgtcgacgaa cgcgcagccc
     1201 atggcggcga catgacgcgg gctcgtggcc agcggtgtgt cgagataccg gcgagtcagg
     1261 ttgctcagca cctgtgcgct cgcctggatc ggaaaatccg gccgggtttc ggaagcgacc
     1321 ggaaccagac tgccctggcg gtcgcgcatc tgccagaaac catcgcgatg ctcgagatgc
     1381 aggtcgaccc gggcgagatg cgtgcccagc gctttcggtt gcagaacagg aataccagcc
     1441 actgaggtgg cgagcagttc gtgagtgtgt ccgagaatga gagcatcgat gcccgcaact
     1501 tcgcgtacca ggcgaaatcc gatgttgcct tcccaggacg attcgcgaat ccgctgcccg
     1561 gtgcgactgt tcacacccga accgagatgc gcggtcagaa tgatgacatc ggcaccagcc
     1621 ttgcgtgctt cgttcacggc caggcgagct ggtacaatcg gatcgtcgaa ggtcagtccg
     1681 gcgtaggatt ccgggtcggc ccagtgagga attcccggcg tggtcacccc gacgacggcg
     1741 atgtgaaccc cctgccgttc gaacatgacc caggggcgga agaaccgttt cccggtggcc
     1801 cggatattgg ctgccagaaa cggaaaactg gcctgcgcgg ctgccgaagc cagcacggcg
     1861 aggtcggcgt cgaactcgtg gttgccgatg gtcagcgcgt cgtagcccat cagattcatg
     1921 gcctgaatca ccgggttggg agtatcgggg gtgaaccgct ggtggtagtg cgtgagtgcg
     1981 ctgccgaaca gggtatcgcc gttatcgagc agcagcgcgc gcgggtgtcg ctcgcgaatc
     2041 tggcgcacca gtgtcgacag acaggccaga ccccg
//
LOCUS       DAORHD010000960         2074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_67938_length_2074_cov_3.983655, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000960 DAORHD010000000
VERSION     DAORHD010000960.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67938_length_2074_cov_3.983655"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="PKO06_17505"
     CDS             <1..1392
                     /locus_tag="PKO06_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71505.1"
                     /translation="QKLARTQADCTGLERGKASSDAEMAALKLKLHERSKTPAGSADV
                     PASDRLEWQRRVKELEELVSRRDKAAQRYEEELAQVRRELDGVQEAGRRDDRRRADQD
                     DAQRQLQRWQNARLARLYHRWQEGDRGSPHLVWEVLDEGFEITPCTPADCAARTEGEF
                     VLLFAATDAVNGDVTHTWHPLEKPREDGMIGVMQPGENGAALVTLEAEYPIQTALPPI
                     EPGTPMAGRWLSAFGERPAGVRALRALRVGPDSSPETKVSFTALAEFLQLDQASPEAL
                     RRHLEQLNGAWQVFPDHVEFGRDARLGASGLRLCLPIVVVCDRPSCRQAKSAHDLVRH
                     ARAGELCHVCHEEVGPAEVEQVRETWNFHDARVLLIGGDEVGSTFETIAADHRLQITW
                     LSGFGHLEPHAAGLETYDLVAVVVRQVSHTLVRELTSAVRRRHARLAFLKRRGVTGVL
                     RELAGLLKASREG"
     gene            1469..>2074
                     /locus_tag="PKO06_17510"
     CDS             1469..>2074
                     /locus_tag="PKO06_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV71506.1"
                     /translation="MAHSNTRRLNPSDEIIKQYFRELKRFPPMSRKEELQLLTRIRNG
                     DVRARRRLVMANLRFVVAVAKKYKFINDVPFEDLIAVGNLGLMRAMKRFDVDHNVRFI
                     SYAVWWIRQAIIQTISLYSNAVRLPVSRVHQGLKIKKTEEQLSKRLNRKPTLNELAQE
                     CGMNPDDLRKFFTDTPVVVSMDGTRVENEDEDLMLSDAISDN"
BASE COUNT          411 a          589 c          669 g          405 t
ORIGIN      
        1 cagaaactcg cccgaacgca agctgattgt acgggtctcg aaagaggaaa ggccagttcc
       61 gacgcggaaa tggctgcgtt gaagttgaaa ctgcacgaac gctcgaagac tccggcgggt
      121 tctgcggacg ttcccgcgag cgatcgcctg gaatggcagc gtcgggtgaa ggaactggaa
      181 gaactggttt ctcgccgtga caaggcggcg caacgctacg aagaagaact ggcccaggtg
      241 cgccgcgagc tggatggcgt tcaggaagcg ggacggcgtg atgaccgccg acgggcggac
      301 caggacgacg cccagcgaca gctgcagcgt tggcagaacg cgcggttggc ccgcctgtat
      361 catcgctggc aggaagggga tcgtggctct ccgcatctgg tctgggaagt ccttgacgag
      421 ggattcgaga ttacaccctg cacgccggca gactgtgccg ctcgcaccga gggtgagttc
      481 gtgcttctgt ttgcggcgac cgatgccgtg aacggtgacg tgacgcacac ctggcatccg
      541 ctggagaagc cccgcgagga cggcatgatc ggcgtgatgc agccgggcga gaacggcgcg
      601 gcgctggtga cgctggaggc ggaatacccc attcaaacag cactgccacc gatcgaaccg
      661 ggcactccaa tggccggccg gtggctgagt gcatttggcg aaaggccggc tggagtgcgc
      721 gcgctgcgcg ctttacgtgt cggccccgat tccagccctg aaacgaaagt ctcgtttacc
      781 gctctggctg aatttctgca gctcgatcag gcgagtcccg aggcactccg tcgacatctc
      841 gaacagttga acggagcctg gcaggtgttt cccgatcatg tcgagttcgg tcgtgatgcc
      901 cggctgggtg cttccggatt gcgtctctgc ctgccgattg tggtggtctg cgatcgtccg
      961 agttgccgtc aggcgaaatc tgctcacgat ctggtgcgac atgctcgtgc tggagagctt
     1021 tgtcatgtct gccatgaaga ggttggcccc gctgaagtgg aacaggtccg cgagacctgg
     1081 aactttcacg atgcccgcgt gctattgatc ggaggcgatg aggtcggttc caccttcgag
     1141 accatcgcgg ccgaccatcg gctgcaaatc acctggctga gcggctttgg ccatctcgaa
     1201 ccgcatgccg ccgggctcga gacctacgat ctggtggcgg tcgtcgtgcg tcaggtatca
     1261 cacaccttgg tgcgtgagtt gacctccgcg gtccggcgcc gacatgcccg actggcgttt
     1321 ctgaaacggc gcggggtgac cggggtgctg cgcgagttgg ccggcttgct gaaggcttcc
     1381 cgcgaagggt aaacaaaccc tcccgttcag ccgataggga aggggggatc gcctgcctga
     1441 aaccgggcgg tggttttccg ttgaacctat ggcacattcc aacacgcgac gtctgaatcc
     1501 atccgatgag atcatcaagc agtattttcg cgaactgaag cggtttccgc cgatgtcgcg
     1561 caaagaagaa ctgcagctgc tcacgcggat tcgcaacggt gatgtgcggg cccggcggcg
     1621 tctggtcatg gccaatttgc gctttgtcgt cgccgtcgcc aaaaaataca agttcatcaa
     1681 cgacgtgccg ttcgaggatc tgatcgccgt cggaaacctt ggtctgatgc gggcgatgaa
     1741 acgattcgat gtcgatcata atgtgcggtt catctcatat gccgtctggt ggatccgcca
     1801 ggccatcatt cagaccattt ccctctactc gaatgcggtg cggctgccgg tcagtcgcgt
     1861 gcatcagggc ctgaaaatca agaagaccga agaacagctc agtaagcggc tcaaccgtaa
     1921 gccgaccctg aatgaactgg cccaggaatg tggcatgaac cccgatgacc tgcggaagtt
     1981 ctttaccgat accccggtgg tcgtgtcgat ggacggcacg cgcgtcgaga acgaagacga
     2041 agatctgatg ctgagcgatg ccatttccga caac
//
LOCUS       DAORHD010000961         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68007_length_2073_cov_3.656591, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000961 DAORHD010000000
VERSION     DAORHD010000961.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68007_length_2073_cov_3.656591"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PKO06_17515"
     CDS             <1..579
                     /locus_tag="PKO06_17515"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HNV71507.1"
                     /translation="AALLTGGGIDHIRITMDEGGLCVTYGGWRPLVLSAGYLGSLLWG
                     TVLLIAAARTRFDREIVATLGGIVILVTLVYIRNLSGFAFGALFGVGLLALAKYAGEW
                     TCDQFLKYLGLTSSLYVVFDIKSDLIDRNIPISDAYRLAEMVYLPGWVVGIVWLVIAV
                     AILWQAMKFALHPGSSPAMPFPLPVERKEIES"
     gene            576..1226
                     /locus_tag="PKO06_17520"
     CDS             576..1226
                     /locus_tag="PKO06_17520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV71508.1"
                     /translation="MTCTCKTVLWDWNGTLFNDAHLCVEIMNRQLSHRRLPTLTLDRY
                     RDLFDFPVRIYYDRLGFDFERESFEVVGTEFIRLYESRKYEAGLRSDTRQVLEALRQK
                     GIGQAVLSAYKHDTLVELIEHFQLGGYFTHLSGLDNHYAHSKVDNGLELIKQLGSDPQ
                     HTVMIGDTIHDYEVAQAMGTGCILVEGGHHPRYKLDSCKVPVVPTLEAALKQIIGR"
     gene            complement(1290..2009)
                     /locus_tag="PKO06_17525"
     CDS             complement(1290..2009)
                     /locus_tag="PKO06_17525"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HNV71509.1"
                     /translation="MLRPPDFKPGRKYPAILEIHGGPHAQYGCTFFHEFQLLAAQGYI
                     VFYSNPRGSKGYGEKHCTSIKGDWGNLDWLDLQAVISFMFGMPEVDTKRLGVMGGSYG
                     GYMTNWIISHTDVFKAAITDRCVSNLVSMSGSSDYPNIPDSYWPGNGWSEIESIWNQS
                     PIRYFDEVQTPTLIIHSEGDLRCNIEQAEQVFTALKIRGVPTRFVRYPASTSHGLSRN
                     GPADLRLHRLNQILEWWKKYL"
BASE COUNT          418 a          557 c          629 g          469 t
ORIGIN      
        1 gcggctctgt tgaccggagg cggcatcgat catatccgca tcaccatgga tgaaggcggg
       61 ctctgcgtca cctacggagg ctggcggccg ctggtactct cggccggtta tctcggcagt
      121 ctcctctggg gaaccgtgct gctgatcgcc gcggcgcgca cgcgttttga tcgtgagatc
      181 gttgccacgc tcggcggcat cgtcattctg gtcacgcttg tctacattcg taacctgtca
      241 ggattcgcat tcggtgcatt gtttggcgtt ggtctgctgg cactggcgaa gtatgcgggg
      301 gaatggacgt gcgatcagtt tctgaagtat ctggggttga cctcgagtct gtatgtggtt
      361 ttcgatatca agtctgacct gatcgatcgc aacatcccta tatctgatgc ctacagactg
      421 gcggaaatgg tctacctgcc cggctgggtg gtcggcatcg tctggctggt gatcgcggta
      481 gcgattctct ggcaggcaat gaagttcgct ctgcacccgg gttcgagccc cgccatgccg
      541 ttcccgctcc ccgttgaacg aaaggaaatc gaatcgtgac ctgtacctgc aagaccgtcc
      601 tctgggactg gaacggaaca ctcttcaacg atgcgcatct atgtgttgag atcatgaacc
      661 gccagttgtc gcatcgccgg ctgccgacac tgacactcga ccggtaccgc gacctgttcg
      721 attttcccgt gcgcatttac tacgatcgcc tggggtttga tttcgaacgc gagtcgtttg
      781 aggtggtcgg cacggagttt attcgtttgt acgagtcgcg aaagtacgag gcgggtttga
      841 gaagcgatac ccgacaggtt cttgaagccc tgagacagaa agggatcggg caggctgtgc
      901 tttcagccta caaacacgat acgctggtcg aactgatcga gcactttcag cttggcggct
      961 acttcaccca cctttccggt ctcgacaatc attatgcgca cagcaaggtc gacaacggtc
     1021 tcgagctgat aaaacagttg gggagtgacc cgcagcatac ggtgatgatc ggcgatacca
     1081 ttcatgacta tgaggtggcg caggccatgg gtaccggctg cattctggtt gaaggcggtc
     1141 atcatccacg gtataagctt gattcctgca aagtaccggt ggtgccaact cttgaagcag
     1201 cgctgaagca gattatcggt cgctgagaaa cccttcaaaa aaccaaccgc cctgccatgg
     1261 cagggcggtt ggtttgagcg ggaacaagct tacagatact ttttccacca ctcgagaatc
     1321 tggttgaggc ggtgaaggcg cagatcggca gggccgttgc ggctcaggcc gtgactggtc
     1381 gaggccggat accgcacgaa gcgggtgggg accccgcgaa tcttgagagc ggtaaagacc
     1441 tgctcggcct gctcgatgtt gcagcgcagg tcgccttcgc tgtgaatgat caacgtcggc
     1501 gtctgcactt cgtcgaaata ccgaatcggg ctctggttcc agattgattc gatctcgctc
     1561 cagccattgc ccggccagta cgaatcggga atattcgggt agtcactcga tcctgacata
     1621 ctgaccagat tactgacgca gcggtcggtg atggcggctt tgaaaacatc ggtgtgactg
     1681 atgatccagt tggtcatgta gccgccgtaa ctgccgccca tgacgccgag acgcttggta
     1741 tcgacttcag gcatgccgaa catgaacgag atgacggcct gcagatcgag ccagtcgagg
     1801 ttgccccagt cgcccttgat cgaggtgcag tgcttttcgc catacccctt gctgccgcga
     1861 gggttgctgt agaagacgat gtatccctgg gcggcgagca gctgaaactc atgaaagaac
     1921 gtgcagccat actgcgcatg cgggccgcca tgaatctcga gaatggcggg gtatttgcgc
     1981 ccgggcttga agtcgggtgg gcgcagcacc cagacctgtg tctcagactc gtcattggcc
     2041 ggaacccagt aggattaggg ggcagaaagg cgc
//
LOCUS       DAORHD010000962         2073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68009_length_2073_cov_3.542616, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000962 DAORHD010000000
VERSION     DAORHD010000962.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68009_length_2073_cov_3.542616"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1499)
                     /locus_tag="PKO06_17530"
     CDS             complement(<1..1499)
                     /locus_tag="PKO06_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71510.1"
                     /translation="MKRAWISNLSLCSLFLSVLLILGCEVPKTSTPSGSTPSATRTDA
                     PAKTETAPASAKPFALQLTSLYRQSIPASNTQMVIEHEGKVRVTVPADAFGKPVEIVI
                     SKPETLPPPTPGCKDLAAYDISVGDKHVFDQLMTIDLSFSPQQIDSDLPPEKALLVSF
                     WNEQTQTWHIAPSLVDVQNGRVTAYTNHLSLWKLTYIARGYRIKESDHFRLIYDPNFR
                     PTLTRPHPKKPTMSITTQEDPDDFAQRVLAALQESHRKYVVAGFTEPDTKIDLGLGEL
                     LKKTVISTVKPSQWVFGFPTTWVFIVPSVLESYRNGLTGSVYISRNFDSPADVSHTCA
                     HEYFHVLQNLYANIYSLGIRKWWVEATAEYAAGQVAFKMGPDVMGDQINYFYPELPLT
                     HKDDVHEYATSHFLHWLVKNGADFRQMWEATMSMKAVLSVIIPLDTYLTKSTHKGLAT
                     HYQEFLAWLLFDPTGPIPDPKNEFATQKHFAKSSLAIGPSESANWDTQVP"
     gene            1686..>2073
                     /locus_tag="PKO06_17535"
     CDS             1686..>2073
                     /locus_tag="PKO06_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71511.1"
                     /translation="MRVLHLLMMFLALLPTMSAAQQAQQLQQLQNPHTQLMNHQVYCA
                     VGASPAALTLSSTFLIEKASVPDAVGLPDGDYLYYVNAEPGKHGIYVARVTTTAIEVL
                     GPVLIDGVYNGNAVDPDVVRLADGRIR"
BASE COUNT          456 a          545 c          614 g          458 t
ORIGIN      
        1 ggaacctgag tgtcccagtt ggcgctttca gagggcccga tagccaggga agatttcgcg
       61 aagtgtttct gtgtggcaaa ctcattcttc ggatcgggta tcggtccggt cgggtcgaac
      121 aacagccagg ccagaaactc ctgataatgt gtggccaaac ctttgtgcgt ggatttggtc
      181 agatacgtgt cgagcggaat gatgaccgac agcacggcct tcatgctcat cgtcgcttcc
      241 cacatctgac ggaaatcagc gccgttcttc accagccagt gtagaaagtg cgaggtggca
      301 tactcgtgaa cgtcgtcttt atgcgtgaga gggagctcag gatagaaata gttgatctga
      361 tctcccatca cgtcggggcc catcttgaag gcaacctgtc cggcggcgta ttcggcggtc
      421 gcctcaaccc accacttgcg gattccaagc gagtagatgt tggcatacag attctgaagc
      481 acgtggaagt attcatgagc gcaggtatgc gacacatcgg cgggagagtc gaaatttcgg
      541 ctgatgtaaa cggatcccgt gagaccgtta cggtacgatt ccaaaacact gggaacgatg
      601 aacacccacg tcgtcggaaa cccgaacacc cattgtgaag gcttcaccgt gctgatcacc
      661 gtctttttca gcagttctcc caggcccaga tcgattttcg tgtcgggctc ggtaaaaccc
      721 gccaccacgt atttccgatg cgactcctgc agggcggcga gcacacgctg ggcgaaatcg
      781 tccgggtctt cctgtgtggt gatggacatc gtcggtttct tgggatgagg gcgcgtgagg
      841 gttggtcgga agttcggatc gtagatgagg cggaagtgat cgctctcttt gatgcggtag
      901 cctcgggcga tgtaggtgag cttccagagt gaaagatggt tggtgtaggc agtgacccgc
      961 ccgttctgaa cgtcgaccaa cgagggagca atgtgccagg tctgcgtctg ctcgttccag
     1021 aacgagacga gaagcgcttt ttccggggga agatcgctgt cgatctgctg cggagaaaac
     1081 gaaagatcga tggtcatcaa ctgatcgaac acgtgcttgt cgccgacact gatatcgtaa
     1141 gccgcgagat ctttacaccc aggggttggc ggcggaagtg tctcgggctt cgagataact
     1201 atctcaacag gtttcccgaa ggcatcagcc ggtacagtca cgcgcacctt tccctcatgc
     1261 tcgatcacca tctgcgtgtt cgaagcggga atagactgtc gatacaacga tgtcaactga
     1321 agcgcgaacg gctttgcaga cgcgggtgcc gtctcggtct ttgcgggcgc gtctgtcctc
     1381 gttgccgacg gggtgctccc ggatggcgtc gacgttttcg gcacctcaca acccagaatc
     1441 aacagaaccg acaggaacag agagcatagc gacaggttac tgatccaggc gcgcttcatc
     1501 tttatacccc cacacctcag cgaaaaatcg atatcaagaa gatggtaggt gttttatctc
     1561 gaaacggcaa gaacgaactc ggaatgtctc ttgaggagaa agcgtttcgt tgccggagat
     1621 ctgggatgtg gacgggggga agcggacgta ataccatgga ggtgaattat gatgaggaga
     1681 atgtgatgag agtgcttcac ctgttgatga tgtttctggc tcttttaccc acgatgtctg
     1741 ccgcccagca ggcccagcaa ctgcagcaac tgcaaaatcc acatacgcag ttgatgaacc
     1801 accaggtgta ctgcgccgtg ggggccagcc ccgccgcgct cactctgtcg tcgacatttc
     1861 tgatcgagaa agcctctgtt cctgatgccg tggggcttcc cgatggcgac tacctgtact
     1921 atgtcaacgc cgaacccggc aaacatggca tttatgtcgc tcgcgtgaca acgacggcga
     1981 tcgaggttct cggcccggtg ctgatcgacg gcgtgtataa cggcaacgcg gtcgaccccg
     2041 acgttgtgcg cctcgcggac ggccgtatcc ggc
//
LOCUS       DAORHD010000963         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68069_length_2072_cov_3.331681, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000963 DAORHD010000000
VERSION     DAORHD010000963.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68069_length_2072_cov_3.331681"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..920)
                     /locus_tag="PKO06_17540"
     CDS             complement(3..920)
                     /locus_tag="PKO06_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71512.1"
                     /translation="MLFLKRTVPLIITFFTGIIVILAFFSGPAMPVKGLDEKASEWAQ
                     VVMTFTMVLGAVSLFQINLFKISAKREGWQFNVVLLAGFCIMAILSFLHGIQTTIVKG
                     GTYYQVNTDKQYTVVDFKLDDKGQQVAALVKSEGDTVPVEMATTNLRGSFDNLIDKWW
                     KFFFDGVFKAANATMFSLLAFFVASASFRAFKVKSREAALLMVAAFVVMLGNVPVGFS
                     VSSFLSWLLWPVLAVITKVIQAVGGLFRIPVTTEVLTVDLAAIKEWIMKVPSSAAQSA
                     ILIGATLGYISAALKVILGVERSYLGGEK"
     gene            complement(930..2018)
                     /locus_tag="PKO06_17545"
     CDS             complement(930..2018)
                     /locus_tag="PKO06_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71513.1"
                     /translation="MAASPNVTAPAVAPANASPAVSAASTPAAEAPVAAPMASAAGSA
                     VAPSVAPAPVSTPVLSTATASGAAAASATPALTEEEKAEAAQKEAEKNNLLNHKRINV
                     AVIILILFALVTYYTQKASAGKEMFIRRISGLAAIDDAVGRATEMGRGVLFIPGIMDM
                     DDIQTIAGVTILGHVAKKTAEYDTALLAPMTRAFTMSVAQEVVYQSYVEKGRKDAYRK
                     DRINYLTDDQFGFVAGVGGIMMREKPAACFYLGTFYAESLILAETGNSVGAIQIAGTA
                     EPSQIPFFVAACDYTLIGEELFAASAYLSRNPEEVGSLKGQDASKLMIILMIVIGVFL
                     VTVSRNLPGDTTTAKSAANFYLDLLKTE"
BASE COUNT          460 a          603 c          608 g          401 t
ORIGIN      
        1 cctcactttt ccccgccgag gtaggaacgc tcgacgccga gaatgacctt gagcgcggcg
       61 ctgatatacc ccagcgttgc accgatcaga atggcactct gagcggctga actgggaacc
      121 ttcatgatcc attccttaat cgcagccaga tcgacggtca acacttcggt cgtcaccgga
      181 atgcggaaca gaccaccgac cgcctgaatc actttcgtga tgaccgcaag cacaggccac
      241 aacagccagc tcaggaacga gctgacggaa aagccaaccg gcacgttgcc caacatgacc
      301 acgaacgcgg caaccatgag cagagccgct tctcgtgatt tgaccttaaa agcgcggaac
      361 gaggccgaag ccacgaagaa agcgagaagc gaaaacatcg tggcgttcgc ggctttgaac
      421 acgccgtcaa agaagaactt ccaccatttg tcgatcaggt tgtcgaatga cccgcgcaga
      481 ttcgtggtag ccatttccac tggtacggta tcgccttcgc tcttcacgag agccgccacc
      541 tgctggccct tatcgtcgag cttgaagtcg accaccgtat actgcttgtc ggtgtttacc
      601 tgatagtagg tgccaccttt gacaatcgtg gtctgaatgc catgcaggaa gctgagaatc
      661 gccatgatgc agaagccggc cagcaacacc acgttaaact gccagccctc gcgcttggcg
      721 ctgattttga acaggttgat ctgaaacagg ctgacagcgc cgagaaccat ggtaaaggtc
      781 atgacgacct gtgcccattc cgacgccttc tcgtcgagcc ccttcaccgg catcgccggg
      841 ccgctgaaga acgcgagaat gacgatgatg cccgtgaaga aggtaatgat cagtggtacg
      901 gttcgcttga gaaatagcat aatcgatcgt tactccgttt tcagaaggtc gaggtagaag
      961 ttcgccgcag acttcgcggt ggtcgtgtca ccgggcaggt tgcgtgaaac ggtcaccagg
     1021 aagacgccga tgacgatcat gagaatgatc atcagcttgc tggcatcctg acccttgagt
     1081 gaaccgactt cttccgggtt gcgcgagaga tacgccgagg cagcgaagag ttcttcgccg
     1141 atcagagtgt agtcgcaggc agcgacaaag aacggaatct gagaaggctc ggcggtgccg
     1201 gcaatctgaa tcgcgccgac cgagttccct gtttcggcaa gaatcagcga ttcagcgtag
     1261 aaggtgccga gatagaaaca ggccgcgggt ttttcacgca tcatgatgcc gccgacaccg
     1321 gcgacaaaac cgaactggtc gtcagtcagg tagttgatgc ggtctttacg atacgcatcc
     1381 ttgcggcctt tctcgacata cgactggtac acgacttcct gcgccaccga catggtgaac
     1441 gcacgagtca tgggggccag cagcgcagtg tcgtattccg ccgtcttctt ggcgacgtgg
     1501 ccgagaatcg tgacaccggc gatcgtctga atgtcgtcca tgtccatgat gccgggaatg
     1561 aagagcacgc cgcgacccat ttcggtggcg cggcccacgg catcgtcaat cgcggccaga
     1621 ccgctgatgc ggcgaatgaa catctctttt ccagcgctgg ctttctgcgt gtagtaggtg
     1681 accaacgcaa acagaatcag aatgatcacc gcgacgttga tgcgtttatg gttcagcagg
     1741 ttgttcttct ctgcttcctt ctgcgccgct tctgccttct cttcttcggt cagagcggga
     1801 gtcgcagagg cagctgccgc tcccgaagct gtcgccgtgc tcaacaccgg cgtcgacacc
     1861 ggagcaggcg cgacggaagg agccacagca gacccggcgg cagaagccat cggtgcggcg
     1921 accggtgctt cagcagcggg agtggacgcc gcagacacag caggcgacgc attcgctgga
     1981 gccaccgcag gcgctgtcac gttcggtgag gcggccacgg ggtcgatcgg cggcgcggct
     2041 accggtgcgg ctggctgagc cgtctccgga gc
//
LOCUS       DAORHD010000964         2071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68133_length_2071_cov_2.687004, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000964 DAORHD010000000
VERSION     DAORHD010000964.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68133_length_2071_cov_2.687004"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1204
                     /locus_tag="PKO06_17550"
     CDS             104..1204
                     /locus_tag="PKO06_17550"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNV71514.1"
                     /translation="MIRLLYAHHPELLAQGLAQRLEREPDAERDLLHPFEIVVPNRNL
                     KVWLTYELTRRLGAVGHLRFRFLSEFLTGAVSVETPVPPLMNQDTLMLRLLELVDSPE
                     VLANPLLQPVSRYVHPPEADERERELRRLQFVETMAGLFLEYEFSRPRMCEAWSRHEL
                     TTRQPLHRALETWQAELWRLLNQPRHPAPGVELPPLKPLSHRFRHLSAERVTSRSPVH
                     LFAFSYMARGYVECLHQLSEFRPIFLYTINPCREYWEDVETPKEQRRRRSFSVPRVGG
                     SLLEEPSEDPFGLRVDMENPLLQMWGKPGRENIRFLNEAVDCDFESVYPDDCDSPEPC
                     SAACRKTSFGVWHLHPRRMSHRPSQRTAACSC"
     gene            1138..>2071
                     /locus_tag="PKO06_17555"
     CDS             1138..>2071
                     /locus_tag="PKO06_17555"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNV71515.1"
                     /translation="MAPASPQDVSPPVSADGSLQLLTAPSVRREAEVIADEIRRLVYQ
                     ARHEAHPLRFSDIAIIVPPGQRERSFAHLRAVFQETDDIPHNLPHTPEHRQSLEAAAL
                     IFQLPFGEWRRPELLRIMQHRAIVSRRPHLDNESIQGWCDECSVFRGRSFSDQKHTFI
                     NASMYNWDQARTRVALGAFMSPGSSSAPHLVEVGETRLLPASVTAEQWPAAASFLDLL
                     AQLEQLHQDYSAGPRPLANWADTFLHWFRRLLGPYRDEPPGEQRLREQAERAILGMSD
                     LEGARVVQVSPFIAVQMLLSRLEALEGESPRYLSR"
BASE COUNT          392 a          646 c          594 g          439 t
ORIGIN      
        1 ggcgaccgcc tggggagcag cgttgctgct ggtcattctg tcgccgtggc tcaagcgctg
       61 gatgcatggg gtcgagtagc cagcagaccg acgtgacgag ttcatgatac ggctcctgta
      121 tgctcatcat cccgaactgc tggcgcaggg cctggcacag cgacttgaac gagaaccgga
      181 cgccgaacgc gacctgctgc accccttcga gatcgtcgtt cccaaccgaa atctcaaggt
      241 ctggctgacc tacgaactga cacgccgcct gggagccgtc ggacatctgc gatttcgctt
      301 tctcagcgag tttctcaccg gcgcggtctc ggtcgagacc ccggtaccgc cgctgatgaa
      361 ccaggacacg ctgatgctgc gcctgctcga gttggtcgat tcgcccgagg tactcgcgaa
      421 ccctctgtta cagccggtct caaggtatgt gcatccgcct gaagcggacg aacgagaacg
      481 tgagttacgg cgcctgcagt ttgtcgagac catggccggt ctgtttctcg aatacgagtt
      541 ttcgcgacca cgaatgtgtg aagcctggag ccgccatgag ctgaccacgc ggcaaccact
      601 tcatcgcgcc cttgaaacct ggcaggcgga gttgtggcgc ctgcttaacc agccacggca
      661 cccggcacca ggggttgagc tcccaccgct gaaaccactg agtcatcgtt ttcgccatct
      721 ttcagccgag cgcgtcacgt cgcgatctcc agtccatctc ttcgcttttt cgtatatggc
      781 cagaggctat gtcgaatgtc tgcatcaact ctccgagttt cgccccatct ttttgtacac
      841 catcaatccg tgccgggagt attgggaaga tgtcgaaact ccgaaggaac aacgtcgtcg
      901 acgctctttt tccgtgccac gcgttggcgg aagcctgctc gaagagccgt ctgaagatcc
      961 atttggtctg cgcgtcgaca tggaaaaccc gctgctgcag atgtggggaa aaccgggtcg
     1021 agaaaacatc cgctttctga atgaggcggt agactgcgat ttcgagtccg tctacccgga
     1081 cgattgcgac tccccagaac cctgctccgc cgcctgcagg aagacatctt tcggcgtctg
     1141 gcacctgcat ccccgcagga tgtctcaccg cccgtctcag cggacggcag cctgcagctg
     1201 ctgaccgctc cgtcggttcg tcgcgaagca gaggtgatcg ccgacgaaat tcggcgactg
     1261 gtgtatcagg cacggcatga ggcccatcca ctgcgctttt ccgatattgc catcatcgtg
     1321 ccaccggggc agcgggaacg ctcctttgcg catctgcgtg ccgtctttca ggagaccgac
     1381 gatatccccc ataatctgcc gcataccccc gagcatcggc agtcactgga agccgcagcg
     1441 ctgatttttc aacttccgtt tggcgaatgg cgacggccgg aattgctgcg catcatgcag
     1501 catcgcgcca tcgtgagccg acgaccgcac ctggacaacg agagtattca agggtggtgt
     1561 gacgagtgct ccgtatttcg tggccgctct ttttctgatc agaagcacac atttataaac
     1621 gccagcatgt acaactggga tcaggcacgc actcgcgtcg cgcttggcgc tttcatgagc
     1681 cccggttcga gttcagcgcc ccatctggtg gaagtcgggg agactcgatt gttgccggca
     1741 agcgtcaccg ccgaacaatg gcctgccgcc gcgtcatttc tcgaccttct ggcacaactg
     1801 gaacagctgc atcaggacta cagcgccgga ccgcgaccac ttgccaactg ggcggatacg
     1861 ttcctgcact ggtttcgtcg tctgcttggc ccgtaccgcg acgaaccacc gggtgagcag
     1921 cgcctgcgtg aacaagccga gagagccatt ctcggcatga gcgatctcga gggagcccgg
     1981 gtggttcagg tgtcgccttt catcgcggtt cagatgctgt tgtctcggct tgaagctctc
     2041 gaaggcgagt ccccgcgcta tctctcgcga g
//
LOCUS       DAORHD010000965         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68220_length_2069_cov_3.374379, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000965 DAORHD010000000
VERSION     DAORHD010000965.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68220_length_2069_cov_3.374379"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKO06_17560"
     CDS             complement(<1..335)
                     /locus_tag="PKO06_17560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV71516.1"
                     /translation="MRAYIDTDILIWHLRGEEKATKFLKKVSRSSEYELWVGAIQRAE
                     IVFFMRPKEEAATELFLSQFRTAPIDQKIVDTAGEYFRKWHPSHGLEINDALLAATAA
                     ETGGHIFTL"
     gene            complement(660..881)
                     /locus_tag="PKO06_17565"
     CDS             complement(660..881)
                     /locus_tag="PKO06_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006197472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HNV71517.1"
                     /translation="MKAKELDTRFDNGENVSAYLDLSKARRPDLEQKRVNVDFPVWMI
                     RQLDREAERLGVPRQSIIKLWLAERLAKV"
     gene            complement(1073..1483)
                     /locus_tag="PKO06_17570"
     CDS             complement(1073..1483)
                     /locus_tag="PKO06_17570"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71518.1"
                     /translation="MKSFRVSVVAPAHPQIDVEAESVSVPAQTGSLGVRYGHLPLLTI
                     LKVGLVHIRDAEGKDRWLGITGGFFEMMGDHATLLADDLISEDEAYEQNEAYDSRTTL
                     MFPAEFPSPLARYDFARALLRRRLEQSGRRPSPT"
     gene            complement(1485..1742)
                     /locus_tag="PKO06_17575"
     CDS             complement(1485..1742)
                     /locus_tag="PKO06_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNV71519.1"
                     /translation="MIDLPYSLVIEATKDPCFFGFYSPDLAGFSGIGHSIEDCIYKAR
                     TGMREHVDLLREEGMPIPPTTLRPQIVIQNEAPRRKKKLAA"
     gene            complement(1754..1942)
                     /locus_tag="PKO06_17580"
     CDS             complement(1754..1942)
                     /locus_tag="PKO06_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNV71520.1"
                     /translation="MKFSELVRLLEKHGFRLLKEKGSIRYYRKENWPSLVRVDFHGPK
                     EVPVGTCVKILKDAGIKV"
BASE COUNT          453 a          558 c          544 g          514 t
ORIGIN      
        1 ttgagggtga agatgtggcc gccggtttcg gctgcggttg cggcaaggag ggcatcattg
       61 atctccagtc cgtggctggg atgccacttg cgaaaatact caccggccgt gtcgacgatc
      121 ttctggtcaa tgggagcggt tcgaaactgt gacagaaaga gctccgttgc ggcctcctct
      181 ttcggtctca taaaaaacac gatttctgcc ctctggatcg ccccaaccca cagctcatat
      241 tcactggagc gactgacttt tttcagaaac tttgttgctt tttcctcgcc ccgaagatgc
      301 cagatcagaa tgtctgtgtc gatgtaagcc ctcatcgacg gttcctctgc atcgactccc
      361 ggaacctgcc acgaacctga gcgaccgtct catccagtgt ttcggcacgt tcccagctcc
      421 cacacgtttc gtcgaaaacg ttggtttctg tttcccgctc ataatgctgc cccagcaaac
      481 gtatggcctg ttcaatgatc gcttttttgg tcgtgtgcat cttcacagac aaatcattga
      541 tcttctggaa gaccgccgga tctatgcgcg cagaaaatat tctatccata gaaattctcc
      601 tccttacgta agtatactta cgtaagaaac cggagtcaac gtgaaaaacg agtttaccct
      661 cagactttgg cgaggcgctc ggcgagccag agtttgatga tcgactggcg cggtacgccc
      721 agacgttcgg cttcgcgatc aagctgacga atcatccaga cgggaaagtc gacattgacg
      781 cgtttttgct cgaggtcggg gcgtctggcc ttcgagagat cgagatatgc cgagacattt
      841 tcaccgttat caaaccgagt atcaagctct ttcgctttca gacaaattga cctcctcatt
      901 tcacgaccgt cgaaccgaga tgattcgttt ttgatcggcc ctgtaagtga tgatggcgga
      961 ccagaacttc tcgacaactt ttccgacaat catcagacgc acttcgtcga tcgttttcac
     1021 gtgaacttcg attcgatgag agtccagctt caatcgtctc tgcgcaatct atctaagttg
     1081 gcgatgggcg gcggccggat tgttcgaggc ggcggcgcag gagggcgcgg gcgaagtcgt
     1141 agcgggcaag cggagaggga aactcggccg ggaacatcag ggttgtgcga ctgtcgtagg
     1201 cttcgttctg ctcgtaggct tcatcttcgc tgatcagatc atcggcgagc agggtggcgt
     1261 ggtcgcccat catttcaaaa aagccgccgg tgatgccgag ccagcggtct ttgccttccg
     1321 cgtcgcgaat atgtaccagg ccgactttaa gaatcgtcag cagcggcagg tgcccgtagc
     1381 gaaccccgag cgagccggtc tgcgccggaa cgctgaccga ttccgcctcg acatcgatct
     1441 gcggatgagc gggtgcaacc accgaaaccc gaaatgactt catgctatgc cgccagtttt
     1501 ttcttgcgtc gaggggcttc gttttgtatg acgatctgcg gcctgagtgt cgttggcggg
     1561 atcggcatcc cttcctcgcg gagaagatct acatgctcgc gcatgcctgt gcgggctttg
     1621 tagatgcagt cctcgattga atgcccaatt cctgaaaatc ctgccaaatc gggtgaatag
     1681 aagccaaaaa agcaagggtc ctttgttgcc tcgatgacaa gagagtaagg aagatcaatc
     1741 atgacaatgt ccttcagact ttgattccag catctttgag aattttaaca caggtgccga
     1801 caggaacttc cttcggacca tgaaaatcaa ctcgaacaag actcggccag ttctcttttc
     1861 gataataacg tatcgacccc ttttctttca gcaggcgaaa accgtgcttc tcgagcaggc
     1921 gaaccagctc actgaacttc atgcttcgcc ttttttcttg agttcttcgg cgcggagctc
     1981 gacgtcgcgg atgctgccgg ccatgtagaa ggcctgttca gggaagtcat catacttgcc
     2041 ttcgatgatc gccttgaatg aggcgaccg
//
LOCUS       DAORHD010000966         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68238_length_2069_cov_2.530288, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000966 DAORHD010000000
VERSION     DAORHD010000966.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68238_length_2069_cov_2.530288"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1916
                     /locus_tag="PKO06_17585"
     CDS             <1..1916
                     /locus_tag="PKO06_17585"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HNV71521.1"
                     /translation="LVVAVLRPQLVLPQSLAQIGCLGFAASGILEWSGGAPSIVALSH
                     FATGIMILQVFSMHDVRNASLALLLSCMLVLTAGAMNVNFFYPLAFFPFVVLFCLSLQ
                     SLARAQQQADASSSFGRVVVASQASLLQWALFFVLWLGFFYLVPRGDAIAIGPSVVNQ
                     RIKGFSQTLRLGEVGSVLENTMVVMRIRPIADFPITGPTLRHLRGLLLRGRSYSRYTG
                     GAWTREGYRTYSYPAYTQRGFVSVDKKLEYGRIKLVLDVLQENTDPPVLFVPVSASAI
                     TTRRPFLNADSDGSLTFDRRNAGIESYQAHIEVGTPDLSEIPYPDWKTVPVDLAPFFD
                     IEDISPLVEDVARDVASGARTIPERIERTIKYLQETCQYSLEEPESQIRDPIAKFLFI
                     TRAGTCEHFASAMTLLLRSMGIPARPVSGYMMNEWNEYGGFFTVRQSDAHAWVEVYWP
                     ELGWIPFDPTPPQTDTLLYSRIDRIIGFRELWNQFEAHWFNLVYRFDRRAQEAGFRKI
                     WASLHANAGGTLILLVLAILVSVLRRWYVRRTLRKPDSDNHRWIPEWFRVWEQTRPNP
                     RRANETPREYLYRLRADVSCTGCQVEDIDMVVSEIRGTLLDGSLSSPCSEPCRQALHR
                     LGQAMKRKAEESL"
BASE COUNT          398 a          625 c          545 g          501 t
ORIGIN      
        1 cactcgttgt ggccgttctg cggccgcaac tcgttcttcc ccaatccctg gctcagatcg
       61 ggtgtcttgg attcgccgcc agcgggattc tggaatggtc aggtggagca ccctcgatcg
      121 tggcgctttc gcattttgca accggcatca tgattctgca ggttttttcg atgcatgacg
      181 tcagaaacgc ttcactcgcg cttctgcttt cgtgcatgct ggtactgacc gccggcgcca
      241 tgaatgtaaa cttcttttac cctctcgctt tttttccatt tgtcgtcctc ttctgcctga
      301 gtctgcagtc actcgctcgt gcccaacagc aggctgacgc ctcatcgtcg tttggtcgtg
      361 tggttgtcgc ttcgcaggcg agcctgcttc agtgggctct cttctttgtt ctgtggctgg
      421 gcttcttcta tctcgtgcct cgtggcgatg ccatcgcgat cgggccatcg gtcgtcaatc
      481 agcgcatcaa ggggttctcg caaactctgc gtctgggcga agtcggcagc gtgcttgaga
      541 acaccatggt cgtcatgcga atacgtccga ttgccgactt cccgataacg ggtccgaccc
      601 tgagacacct gcgcggtctg cttctgcgcg ggcgaagcta cagccggtac acggggggcg
      661 cctggacccg cgaaggatat cgcacctact cctacccggc gtatacccag cgaggattcg
      721 tctccgtgga caagaagctg gagtatggca gaatcaaact ggtgctcgat gttctacagg
      781 aaaacacgga tccccccgtg ctgtttgtcc cagtcagcgc ttctgccatc accacccggc
      841 gcccctttct caacgccgat tccgacggat cactgacatt cgaccgacgg aatgccggaa
      901 tcgaaagcta tcaggctcac atcgaagtgg gcactcccga tctttccgaa atcccctacc
      961 ccgactggaa aaccgttccg gtcgatctcg cccccttctt tgacatcgaa gacatctcgc
     1021 cgctggtaga agacgtggca agagatgtgg cttccggtgc tcgcaccatc cctgagcgca
     1081 ttgagcgaac catcaaatat ttacaggaga cctgtcagta ttcgctcgaa gaacccgaat
     1141 cgcagattcg cgacccgatc gcgaagtttc tcttcatcac gcgcgccggc acctgtgaac
     1201 actttgcctc agccatgact ctattgctgc gatccatggg aattcccgcg cgcccggtca
     1261 gcgggtatat gatgaatgaa tggaacgagt acggcggatt cttcaccgtt cgtcagagcg
     1321 acgcccacgc atgggtggaa gtgtattggc ctgagctcgg ctggattccg tttgatccca
     1381 ccccacccca gaccgatacg cttctgtatt cgcgcatcga tcgcatcatc ggctttcgag
     1441 aactctggaa ccagttcgaa gcccactggt tcaatctcgt ctatcgcttt gaccgccggg
     1501 cccaggaagc gggttttcgc aaaatctggg caagcctgca cgcgaatgct ggcggaacgc
     1561 tgatcctgct ggttctcgcc atcctcgtat ctgttcttcg cagatggtat gttcgccgca
     1621 ctctccggaa acccgactca gacaatcatc gctggattcc cgaatggttc agggtgtggg
     1681 agcaaacacg cccgaatcct cgccgtgcca atgagactcc tcgagagtat ttgtaccggc
     1741 tacgggcaga tgtttcatgc acaggatgcc aagtcgaaga catcgatatg gtggtttcag
     1801 aaatccgcgg caccctgctc gatggcagtc tatcttcccc ctgttccgag ccttgtcggc
     1861 aggccttgca tcgacttggt caggcaatga agagaaaagc ggaagaaagc ttgtagctct
     1921 cttccgcttt tttcactgtg gccgacgttc gcgtcggaaa gatcaggtta cttgccagtc
     1981 tgctgtttca ccagcggcaa ctgcccacca gctttaatca tctgaacatc gaggtcactg
     2041 agggtatgag tcaccgggat cttgcgacc
//
LOCUS       DAORHD010000967         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68262_length_2068_cov_3.679583, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000967 DAORHD010000000
VERSION     DAORHD010000967.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68262_length_2068_cov_3.679583"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..1410)
                     /locus_tag="PKO06_17590"
     CDS             complement(343..1410)
                     /locus_tag="PKO06_17590"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNV71522.1"
                     /translation="MSKIKVLVVDDSATIRMLLSKGLNLDPELEVVGTAPDPYVARNK
                     IVELSPDVITLDVEMPRMDGIEFLRRLMAAYPLPVVMVSALTEKGKRITLEALDAGAF
                     DFVHKPSGSHGAQGLQDMLTDLRQKIKSAARANLTTLRKSLNRVTAPIARPLAPVRPT
                     ALAETTDKVVAIGASTGGTTAIRDMISMLPADFPGTVLVQHITPGFTTMFAERLNEIG
                     TVQVKEAQHGDRIITGRVLVAPSGHQMTVRRSGGFYYVDCVPGPPICGHIPSVEALFQ
                     SVAKQVGANALGVILTGMGGDGADGMAAMRKAGARTLGQDEASCVVFGMPKVAWEKGG
                     VEKLLPLEEIVPNLLRLLRAG"
     gene            complement(1426..>2068)
                     /locus_tag="PKO06_17595"
     CDS             complement(1426..>2068)
                     /locus_tag="PKO06_17595"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNV71523.1"
                     /translation="TSGNEIVFLLDAISTNVTSFYRESHHFRLMEKILAGWVHEGQKR
                     FRVWCAASSTGEEPYTLAMTMAEICQSPAIDWKILATDISTKVLELAHAGVYRSEKLD
                     VVPAQLKQKYFDCSRDAAGDKVFHARQILRDHLMFRRLNLRETPFPMKGPMDFIFCRN
                     VMIYFDNAVRQPLIAEFHRLLKRGGILCIGSSESLTGLKTDMQNLGPSVYVKP"
BASE COUNT          423 a          602 c          611 g          432 t
ORIGIN      
        1 tgagggtatc acgcggatcc cggaaaagtc aaaaactccg cggtttctcg ccgggtcatg
       61 tgattctccg gcttgggttt tccccctggc atcgctcaac gattcgccgt cgcggcaggg
      121 agtgcgccca tcatctcgaa acgcaggttg aatccttctc gacttctcat ccctgtgaag
      181 accgagaagg ccgaatcgcc tcctgcggcc cggcagcctg gttccgtact cagtccgcct
      241 cttgccgaaa actcacaagt tttgctcaca ccaagaggag aaccggacag aatcacttga
      301 ccctgagctt gtagaggaag gagggtaggc tgcggtgaaa gtttatccgg cacgcaacag
      361 gcgcagcaga ttgggaacga tttcttcgag gggcagaagt ttttcgaccc cccctttttc
      421 ccaggcgact ttgggcatcc cgaaaaccac gcaggacgcc tcatcctgcc ccagggtgcg
      481 cgcgcccgcc ttgcgcatcg ccgccattcc atccgcgccg tctcctccca tgccggtgag
      541 aatgacgcca agggcgttag cgccgacctg cttggccacc gactggaaca gggcttcgac
      601 cgaagggatg tgtccgcaga tcggtggtcc gggcacgcag tcaacgtagt aaaatccccc
      661 cgatcgccgc acggtcatct ggtgcccgct gggggcgacg agaacccgcc cggtgatgat
      721 gcgatcaccg tgttgcgctt ccttgacctg gacagtgccg atctcgttga gtcgctcggc
      781 aaacatcgtc gtgaacccgg gggtgatgtg ttgaaccagg acggtaccgg gaaaatccgc
      841 cggcagcatg gagatcatgt cgcgaatggc ggtggttccg ccggtcgagg ccccgattgc
      901 caccaccttg tccgtggtct cggcgagagc ggtgggtcga actggtgcca gtggccgggc
      961 gattggcgcg gtgacacgat tcagcgattt gcgcagcgtg gtcagattgg cgcgagccgc
     1021 cgacttgatc ttctgtcgca gatcggtcag catgtcctgc aagccctgag caccgtggct
     1081 gcccgagggt ttgtgcacga agtcaaacgc tccggcgtcg agtgcttcca gcgtgatccg
     1141 ttttcccttc tcagtcagag cgctgaccat gacgaccggc aacgggtatg ccgccatcag
     1201 tcgtcgcagg aactcgatac catccatgcg tggcatttcg acatcgagcg tgatcacgtc
     1261 tggagacagt tccacgatct tgttgcgggc gacgtaggga tcaggggcgg tgccgaccac
     1321 ctcgagctcc ggatcgagat tcaatccctt ggataacagc atccgaatgg tggcagaatc
     1381 atcaacaacc aggactttta tctttgacat gccagcatca cctgatcatg gcttaacata
     1441 gacggatggc ccgagattct gcatgtcggt cttgagaccg gtgaggcttt ctgatgagcc
     1501 aatgcagaga attccgccgc gtttgagcag ccggtgaaac tccgcgatca acggttgtcg
     1561 cacggcgtta tcaaaataga tcatgacatt gcgacagaag atgaaatcca tgggcccctt
     1621 catgggaaaa ggagtttctc gcaggttcag acggcgaaac atcagatggt cgcgcagaat
     1681 ctgcctggca tgaaagacct tgtcacccgc ggcatcgcga ctgcagtcga aatacttctg
     1741 tttgagctgc gccggcacga catcgagttt ttcacttcgg tacaccccgg cgtgggccag
     1801 ttcgagaacc ttggtcgaga tgtccgttgc caggatcttc cagtcgatgg cgggggactg
     1861 gcagatctcg gccattgtca tcgccagcgt gtagggttct tctccggtcg acgaggccgc
     1921 gcaccagacg cgaaaccgtt tctgcccctc atgtacccag ccggcgagga tcttttccat
     1981 caaccgaaag tgatgagatt cgcggtagaa ggaagtgacg ttcgtggaaa tcgcatcgag
     2041 caggaacacg atttcattgc ccgaagtg
//
LOCUS       DAORHD010000968         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68269_length_2068_cov_3.189270, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000968 DAORHD010000000
VERSION     DAORHD010000968.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68269_length_2068_cov_3.189270"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PKO06_17600"
     CDS             complement(<1..422)
                     /locus_tag="PKO06_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV71524.1"
                     /translation="MGKNKDGDKVFSPVPGERVQLPPLPQMLSYLQAGLTAADIARIH
                     GCASSSVYGAIRRYGIDITALRTFKGRRADTLAHIQMLVTNAMPGKVENTSLRDLATT
                     FNILHNAERLERGQSTANVALGDVSGQLSELDATIAKL"
     gene            complement(400..>2068)
                     /locus_tag="PKO06_17605"
     CDS             complement(400..>2068)
                     /locus_tag="PKO06_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71525.1"
                     /translation="PGRTADVSGSDIAPVNIIKNAVRQVGEDLNTLSSHIAAWGGKDP
                     VNMGGVWNNVDDLSKEVLTSIAPPNPHYASTIGRIVEKRKAYSRAGKQFTIADQLQMS
                     KELREAIDGLDPRALREKRNLVLLRNQLKDEVRKNIDSAANAVNDPQTAKSLRDMWDG
                     LNLEYANGTRAAEAFEAASEGGLINPQKLMNEMGARASEMDQIMGIGAMDAATRLSTN
                     PAANLYNKLPAGGETTALYRALKEGNPAAWELIRGQQGGEEWAIRSFLDDSMKSKKTT
                     TGTAQVTERRYDLSKMVEENRDFFDNLSPNGKGLLQKWIENPAMLEPANLEAVRKGIA
                     ATQATPNPVKAVSDLLTSPAVPTDLLQTKLMKDAFKHEAGPMVLNEILDPTGKGTDLA
                     RLEQFVRGAETPGYTGVGTMSREKRMALFTPEQNAAIDRMGTLGKHLMNVLNEWPEES
                     HGKIAQIIREGLSAETAASTAAGQIGLRGVPKTGGAVMGAFVLTAVPRILKYFATTPN
                     SPQKQRALAALTTFLRQAGTRTSQGASAVVAPKSVIGGQKNGQEQRR"
BASE COUNT          369 a          582 c          594 g          523 t
ORIGIN      
        1 tccagcttgg caatggttgc gtcgagttcg gacaactgac ctgagacgtc cccaagggca
       61 acattcgccg tgctttggcc tctttccagc ctttcggcat tgtggaggat gttgaaggtc
      121 gtcgcgaggt cgcggaggct ggtattctcg accttccccg gcatggcgtt ggtgacgagc
      181 atctggatgt gcgccagcgt gtcggcccgg cgacccttga acgtgcggag agccgtgatg
      241 tcgatgccat atcggcggat cgccccgtaa acgctcgacg aggcgcaccc gtggatacgg
      301 gcgatgtcgg cggccgtcag ccccgcttgc agatatgaga gcatctgcgg aagcggggga
      361 agctggaccc tttccccggg gaccggggag aatactttgt caccgtcttt gttcttgccc
      421 atttttttgt ccccctatta ccgatttagg agccacaacc gcactcgcac cctgactcgt
      481 acgagttcca gcctgacgta agaaggtcgt tagggccgcc aaagctcgct gtttctgagg
      541 gctgttcggc gtggtggcga aatacttgag tatccgagga actgccgtga gaacgaaggc
      601 acccatgacg gcacccccgg tcttgggaac gcctctgagg ccgatctgtc ctgcggccgt
      661 cgatgcggct gtttcggcac tcagtccttc gcggatgatc tgagcaatct tgccgtggga
      721 ctcctcgggc cactcattca ggacattcat cagatgcttt ccaagggtgc ccatacggtc
      781 gatggcggcg ttctgttcag gagtgaacag agccattctt ttctctcttg acatcgttcc
      841 gacaccagta tatccaggtg tttcagcccc ccgtacaaac tgttccagac gggcaagatc
      901 agttcctttc ccagtcgggt ctagtatttc attgagcacc atcgggccag cttcgtgttt
      961 gaaggcgtcc ttcatgagtt ttgtctggag aagatcggtc ggaacagcgg gggaagtcaa
     1021 tagatcggag acagccttca cgggattcgg tgtggcttgt gtggccgcta tgccctttcg
     1081 cacggcttcg aggtttgccg gttcaagcat cgcggggttt tcgatccact tctgaagcag
     1141 gcccttgccg ttggggctga ggttgtcgaa gaagtcccgg ttctcttcga ccatcttcga
     1201 caagtcatat cggcgttcag tgacctgagc tgttccggtc gtggttttct tggatttcat
     1261 cgagtcatcg aggaaacttc tgatagccca ttcttcgccg ccctgctggc ccctgatgag
     1321 ttcccatgcg gccgggttgc cctctttgag agccctatac aacgccgtag tttcgccacc
     1381 tgcgggaagt ttgttgtaca gatttgccgc cggattggta gacagcctcg tggccgcatc
     1441 catcgcgccg atacccatga tctgatccat ttcagaggct cgggcaccca tttcattcat
     1501 cagtttttga ggattgatga gcccgccttc agatgccgcc tcgaacgctt cggccgcacg
     1561 ggtgccattg gcgtattcga ggttcagacc gtcccacata tcccgaaggg acttggccgt
     1621 ctgggggtca ttgaccgcat tggcggcgga gtcgatgttc tttcgcacct catctttcaa
     1681 ctggttacga agaagtacaa gatttctctt ctcccgcaat gcgcgggggt cgagcccgtc
     1741 gatggcttcc cgaagctcct tcgacatttg aagctgatct gcaattgtga attgtttgcc
     1801 tgcccgagag taggctttcc gtttttcaac tatgcggccg atagtgctgg cataatgcgg
     1861 attcggggga gcaattgacg tgagaacttc tttactcagg tcgtccacgt tgttccatac
     1921 tccacccatg ttgacggggt ccttgccgcc ccacgccgcg atatgcgacg aaagggtatt
     1981 caggtcttca cccacctgtc gtacagcgtt tttgatgatg ttgacgggag ctatatcgct
     2041 gccagaaaca tccgccgtcc ggccgggg
//
LOCUS       DAORHD010000969         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68440_length_2065_cov_3.271642, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000969 DAORHD010000000
VERSION     DAORHD010000969.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68440_length_2065_cov_3.271642"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKO06_17610"
     CDS             complement(<1..1104)
                     /locus_tag="PKO06_17610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV71526.1"
                     /translation="MRCLFCLLILSLSCILPSGASAEELRWPLDIEISPSSSFMEYRG
                     LRFHGGVDLRTRRAVGFPVFAIADGFVSRLKIQHRGFGYALYVDHPALKLRSVFGHLD
                     DYAEPMAAWAAKKLQKMGKRYGIDDQFGPETFPVKKGQLIAYTGETGLGPPHLHFELR
                     TLNDEPLSPTALGYVIPDRIDPDCTGVWIEPLSHGTMINGGFLPCRIPLTKSEGNFWS
                     WDSPIRIQGRAGLAVGVLDRAEGDNRMSAAEIRLSLDGKEVFSRLFHRYSYDENRQAA
                     YVFDAVRSAAPGLGYVVWMFKWPFENMMLSREHSAWAGVLDESGAGKRLSISIKDFSG
                     REAQVEGPVDVTGIPRRTTPTSDRFLAHDATTSF"
     gene            1389..>2065
                     /locus_tag="PKO06_17615"
     CDS             1389..>2065
                     /locus_tag="PKO06_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV71527.1"
                     /translation="MRHTSLRSVLKLTLWSLFLAAFAFALPGCSIFGGDSDGGTFAGT
                     VDTRTVTGRVLAPDDAPQNITADSIRAAFAGRAGIKVALEELPNISDDTDENGVYVLE
                     NVPVGGPYHVVAWLEPGGQPYRVRSVTITADGDSSVAITVADLQLAKANRVVNGKLVN
                     STGAALPANTKMWLWGKEFFLGDKGLFTSPALPETATQAQIIVDETATTRRYTFTAPF
                     VATAAAT"
BASE COUNT          461 a          603 c          577 g          424 t
ORIGIN      
        1 gaatgaagtc gttgcatcgt gggccaggaa gcggtcgctg gtcggcgtcg ttcgccgtgg
       61 aattccggta acatcaacgg ggccttctac ctgagcttct cgaccactga agtctttgat
      121 gctgatcgaa aggcgtttcc ctgcgccgct ttcatcgagc acaccagccc aggcactgtg
      181 ttccctcgac aacatcatgt tctcgaaggg ccatttgaac atccagacga catagccaag
      241 cccgggtgcg gcggaacgca cggcatcgaa cacgtaagcg gcctgccgat tctcatcata
      301 ggaataccgg tgaaacaacc gggaaaagac ttctttgccg tcgagagaaa gccggatctc
      361 ggcggcactc atgcgattat cgccctccgc acgatcaagg actccaacag caagtccagc
      421 ccggccttga atacgaatcg gtgagtccca gctccagaag ttgccttcgc tcttcgtcag
      481 aggaatacgg cagggaagaa atcctccatt aatcatcgtc ccatgcgata atggctcaat
      541 ccagactccg gtgcaatcag ggtcgatacg atcgggaatg acgtaaccga gcgcagtagg
      601 cgacagaggt tcgtcgttca gcgtgcgcag ctcgaaatgc aggtgtggcg gtccaagccc
      661 ggtttcgccg gtataggcga tcaactgccc cttcttgacc gggaaagtct cgggtccgaa
      721 ctggtcatcg atgccatacc gtttccccat cttctgcagt tttttcgcgg cccaggctgc
      781 catgggctcg gcgtagtcgt cgagatggcc gaaaaccgag cggagcttga gggcgggatg
      841 gtcgacgtac agcgcgtatc cgaacccgcg gtgctgaatc ttcaggcgcg acacgaaacc
      901 gtcggcgatc gcaaagaccg ggaacccgac cgcgcgacga gtacgaagat cgaccccgcc
      961 gtgaaagcgc agaccacggt attccatgaa actcgaactc ggactaatct cgatatcgag
     1021 aggccaacgc agttcttctg ccgacgcacc actgggtaga atacacgaga gagacaaaat
     1081 cagaaggcag aacagacaac gcatacgcgc atcctttcac cgagcgtgat gagatgtgac
     1141 aggtatacct gaaaccgtgg cgtggtacaa gaggttcatg cacttttcga ggtcagcaag
     1201 gtctctgggc tcggctgtct gttgtctggc gcgtcaaagc tgagaaacaa aaatcttgaa
     1261 atgagatgac tgatcgagac ggcatcacgc gaaaagtttg acagtgtttc cggttgttgc
     1321 tacccttgcc ggagtcaatt gcaggtcggt acacccaccg actcggattt atcgggagga
     1381 tacggtccat gcgtcatacg tctttgcgct cggttctcaa gctcaccctg tggtctttgt
     1441 tccttgcggc gttcgctttc gcgctgcccg gttgcagcat cttcggcggc gatagcgacg
     1501 gcggaacgtt cgccggcacc gtcgacacac gcacggtcac cggtcgcgtg ctcgctccgg
     1561 atgatgcgcc acagaacatc accgctgaca gcatccgcgc cgctttcgct ggccgtgccg
     1621 gaatcaaggt cgcgcttgag gagcttccga acatttctga tgacacggac gagaacggtg
     1681 tctatgtcct cgaaaatgtc ccagtcggcg gaccctatca cgttgtcgcc tggcttgaac
     1741 caggtggtca gccctatcgt gtgcgctcgg taaccatcac cgccgatggc gattcttcgg
     1801 ttgctattac ggttgcagat ctgcaactcg ccaaagccaa ccgagtggtc aacggcaagc
     1861 tcgtgaactc aaccggtgct gcacttcccg cgaacacgaa gatgtggctt tggggcaagg
     1921 aattttttct cggggacaag ggactcttca cttccccggc acttcccgag acggcaacgc
     1981 aagctcaaat catcgttgat gaaacggcaa ccacgcgccg gtacacattt accgcaccct
     2041 tcgtggccac tgccgcagcg accat
//
LOCUS       DAORHD010000970         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68487_length_2064_cov_4.929318, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000970 DAORHD010000000
VERSION     DAORHD010000970.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68487_length_2064_cov_4.929318"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKO06_17620"
     CDS             <1..803
                     /locus_tag="PKO06_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV71528.1"
                     /translation="GQKNEGLSVWGFSNYVNTTGTAVSFTIDDGTGANVVVNAYTTVS
                     TAAAADLYKFDVWQASVNRALVTGGAQVRVDQVGSTIAFTALRVGQENPDGAAAIRSL
                     VSLTGDAEFNSLFDIQNGTAMGSGDKNFRVHIVNNSPNFQIGGDPGQKMQVNFANMNA
                     DALGVQALDLTTVSAAAKSIAKINSAIDRVSAERSKLGAFQNRLEYAISNIRNTYTNL
                     SAAESRIRDADVAQEMIEFTRNQIVNQSGTAMLAQANLLPQGVLQLLR"
     gene            1001..>2064
                     /locus_tag="PKO06_17625"
     CDS             1001..>2064
                     /locus_tag="PKO06_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71529.1"
                     /translation="MSLRINQNVLSIRTQGQLAQTSNRLEKSVEKLSSGLRINRAADD
                     AAGLAISEKLRRQIRGLQRATLNAQDGISMIQTAEGALNETQSILQRMRELAIQASND
                     TLTSNDRLEIQKEVNQLRDDINRISRNTEFNTKKLLDGSQTALVSGSTDAVRGIVVGE
                     ASAAAGDYEVSITLVQAGVSEMQRSQTFLLNDGSGLIAKGNTELQSIAQLYDANGVFT
                     LASPQTLTINGNGKSTTITVDGQMTLDKLAASIQNAIVGSSGLELLNTKVGVVNTAQT
                     GLAGLGGYVQITSGFIGDVGKIAFSGDQPLMDALGLSVNRQSAENQVNVTLRDSLGNT
                     KTSLTTDSRVSGLLNGIDLA"
BASE COUNT          478 a          539 c          625 g          422 t
ORIGIN      
        1 aggggcagaa aaatgagggg ctgtcagtct ggggtttctc gaactacgtg aacacgacgg
       61 gaacggctgt ttccttcacc atcgatgacg gaaccggagc gaacgtggtg gtgaatgcct
      121 atacgactgt cagcacagcg gctgccgcag acctgtataa gttcgatgtc tggcaggcct
      181 cggtgaatcg agccttggtc acaggtgggg cacaggtcag agttgatcaa gtcggttcga
      241 cgattgcctt taccgctctg cgtgtcggtc aggagaatcc ggatggagct gcagcgattc
      301 gcagtttggt atcgctgacc ggcgatgccg aattcaacag cctgttcgac attcagaatg
      361 gaaccgcgat ggggagcggc gacaagaact tccgggttca tatcgtgaac aactcgccga
      421 atttccagat cggcggtgat ccaggtcaga agatgcaggt gaacttcgcg aacatgaacg
      481 cggatgctct gggggtgcag gcacttgatc tgaccaccgt ctccgcggcg gcgaagtcga
      541 ttgccaagat caactcggcg attgaccggg tctcggccga acgttcgaaa ctgggtgcgt
      601 tccagaaccg tctggaatac gcgatcagca acattcgcaa cacctacacg aacctgtcgg
      661 ctgccgaaag ccgcattcgt gatgcagatg tagcgcagga aatgatcgaa ttcacccgca
      721 atcagatcgt caatcagtct ggcacggcga tgctggcgca ggcaaatctg cttccgcagg
      781 gagtcctgca actgttgagg taaaaaacat caggcccccc gattttcggg gggccgccca
      841 tttcatctct gatgcctggt tcgtgggccg ggaacctatg agcaacaggc catctgaatc
      901 tggaggtgtc gcagaactat gagctgatgt tgtgatcgca acaatttatc atcgctacat
      961 ccggcatgga ggccgggaat gagttcaagg aggaattcat atgtcgctac gcatcaatca
     1021 aaacgttctt tccattcgca ctcagggcca gttggctcag acgtcgaacc gtctggaaaa
     1081 gtcggtcgag aagctgagtt caggtctgcg catcaatcgc gcggccgatg acgcggcggg
     1141 tctggcgatc agcgaaaagc tgcgccgcca gattcgtggt ctgcagcggg ccacgctgaa
     1201 cgcccaagac ggcatctcga tgattcagac ggctgaaggc gctctgaacg agacgcagag
     1261 cattctgcag cgcatgcgtg aactggcgat tcaggccagt aacgatacgc tgacctcgaa
     1321 tgatcgactc gagattcaga aagaagtgaa tcagctgcgc gacgatatca accgcatctc
     1381 gcgcaatacg gaattcaaca ccaagaaact gctcgatgga tcgcagactg cgctggtcag
     1441 tggtagtaca gacgcagttc gcggcatcgt ggtcggcgaa gcttctgctg cggccggcga
     1501 ctacgaagta agtattactc tggtacaggc cggcgtttcc gaaatgcagc gttcgcagac
     1561 ctttctgctg aatgacggca gtggtctgat cgccaagggt aatacggaac tgcagagtat
     1621 tgctcagctg tatgatgcca acggggtgtt tacgctggcc agtccgcaga cgttgacgat
     1681 caatggcaac ggaaaatcaa ccaccatcac ggttgacggt cagatgaccc tggacaaact
     1741 ggcagcgtca attcagaacg cgatcgtcgg gagctcgggt ctcgaactgc tcaacaccaa
     1801 ggtcggcgtg gtgaacacgg cgcagactgg tttggccggc ctgggcgggt atgtccagat
     1861 cacttcgggc ttcattggcg atgtcggcaa gatcgcgttc tcaggcgatc agccgttgat
     1921 ggacgctctt ggtctgtcgg tgaaccgcca gtcggcggaa aaccaggtga acgtgacgct
     1981 gcgcgacagt ctcggcaaca cgaagacctc gctgacgact gacagtcgcg ttagtggcct
     2041 gttgaacggc atcgatctgg cgtt
//
LOCUS       DAORHD010000971         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68554_length_2063_cov_3.897410, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000971 DAORHD010000000
VERSION     DAORHD010000971.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68554_length_2063_cov_3.897410"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKO06_17630"
     CDS             <1..742
                     /locus_tag="PKO06_17630"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNV71530.1"
                     /translation="LGIYELTTLGVRVACPVGERLRLTAGLFEGTPGTAEQNPHGWNW
                     RLGKHEGVFRIAEMQWQPRMGKKGCEPLIKIGNWYHSGDFADLRQSGNVDANDDDAPA
                     EPIGHRHNWGTYVTYDQQIFRGSTDPDTGLGVFCQKGWMTPNDRSSVESSWGCGFTWT
                     GPFRERTSDALSVGLGRTTYSRSARAFEEENQGAFFVTEEVVEIGYLYQLAPGLTLRP
                     SLQFIKNVEGDPNLSRARYFSLRCTAVF"
     gene            824..1327
                     /locus_tag="PKO06_17635"
     CDS             824..1327
                     /locus_tag="PKO06_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71531.1"
                     /translation="MTERHPESASEMISCPFCGEQIAAAAVLCRFCRSALVDPQGRRL
                     TQDDARRLIQSPRQTAETSVSLGSALAANLFCPGLGSWKLGSRLRGAVVGVVTLLCIT
                     FAVSAYANAFQSEFGKAMRTGRTKALEAKLNEAQDNLWAQAAFWLYVYSFVDVGLIWA
                     GSQKRSE"
     gene            complement(1367..1933)
                     /locus_tag="PKO06_17640"
     CDS             complement(1367..1933)
                     /locus_tag="PKO06_17640"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HNV71532.1"
                     /translation="MRKPLFSRVKQYLIAVLVGGLLGLTACGGGESAKPGEPGKPATG
                     VQKAETPAKPAEAPLLTVGQPGRNGAFEITLTGVEKPTKWINAPKAGRQYIVVKLKIT
                     NVSDQTKSIGAGSFGCVDDANGNRGSYERSTGIQTTPDTFGATEIKPGESFEGSIIFS
                     IPVDMNTTELHYTVGYALKPNLRFEVKL"
BASE COUNT          411 a          562 c          626 g          464 t
ORIGIN      
        1 gctgggaatc tatgagctga ccactctcgg cgtgcgcgtg gcctgccccg tgggtgaacg
       61 tcttcgactg acagcgggcc tgttcgaagg aacgccggga acggctgagc agaatccaca
      121 cggctggaac tggcggctcg gaaagcatga aggagtgttt cgcatcgccg agatgcaatg
      181 gcaaccaagg atgggaaaga aaggatgtga gcctctgata aagatcggca actggtacca
      241 tagtggcgac tttgccgacc tgcggcagtc aggaaacgtg gatgcgaacg atgatgatgc
      301 gccggccgag ccaatcgggc atcggcataa ctggggaacc tatgtcacct atgatcagca
      361 gatctttcga ggctcgacag acccggacac tggcctggga gtcttctgcc agaaaggctg
      421 gatgacgccg aacgatcgct cgtcggtcga gtcttcctgg gggtgtggtt tcacctggac
      481 tggtccattt cgtgaacgga cgtccgacgc gctttcggtc ggtctcggac ggaccaccta
      541 cagtcgctcg gcgcgggctt ttgaagaaga aaaccagggc gcgtttttcg tgacggaaga
      601 agtcgtggag atcggctatt tgtaccagct ggccccgggg ctaacgctca ggccgagcct
      661 gcagttcatc aagaacgtcg agggagaccc gaacctgtca cgagcgcgct acttcagcct
      721 gagatgtacc gcggttttct gatctaccgt ttccccgcct gcgttcatac cggacttcat
      781 cgcggacgtc gattttcaac ggggccaggt gtatagttgc ggcatgaccg aacgacaccc
      841 cgagtcagca agcgaaatga tcagttgccc gttctgtggc gaacagattg ccgcggcagc
      901 ggttttgtgc cgcttctgcc gatcagcgct ggtggatcca cagggtcgac gcctgacgca
      961 ggacgatgcc cgacgattaa tacaatcacc gcgtcagacg gccgagacgt cggtttccct
     1021 gggcagtgca ctggcggcca acctcttctg tcctgggctc ggcagttgga agctggggtc
     1081 aagactgcgg ggcgccgtcg ttggggtcgt tacgctgctc tgcatcacgt tcgcggtatc
     1141 ggcgtatgcg aacgcattcc agagcgagtt cggcaaagcg atgcgcacgg ggcgcaccaa
     1201 ggctctcgaa gcgaaactca acgaggccca ggacaatctg tgggctcaag cggccttttg
     1261 gctctacgtc tattcgttcg tcgacgtcgg gctgatttgg gccgggagcc aaaaacggtc
     1321 tgaatagcga ttcctgctcg tattcagacc tggcggctcg ccccaatcag agcttgactt
     1381 caaaacgcag gttcggcttc agggcgtacc cgacggtgta atgcagttcc gtggtgttca
     1441 tgtcaacggg aatcgaaaag ataatcgatc cttcgaatga ttcgccgggt ttgatctccg
     1501 ttgcgccgaa ggtgtccgga gtggtttgga ttccggtcga tcgctcatac gatccgcggt
     1561 ttccattggc atcgtcgaca cagccgaaac ttccggcacc gatgctcttt gtctggtctg
     1621 aaacattcgt gatcttgagt tttaccacga tatactggcg acctgctttg ggcgcgttga
     1681 tccacttcgt tggcttttca actccggtca aagtgatttc aaatgcgccg tttcgtcccg
     1741 gttgccccac agtcagcagg ggggcttcgg ctggtttcgc gggggtctca gctttctgaa
     1801 cgccggtagc cggtttgcct ggctctccag gcttcgcgga ctcgccgcct ccgcaggccg
     1861 tcagtccgag aagcccgccc acgagaacgg caatcagata ctgtttcaca cgagagaaaa
     1921 gaggtttacg cacgatgaac tccttgggtt aaaggttgat tacttgtact ctttgaacgc
     1981 ggcgagaatc ttttccagat caggatgctc cgtcagtttg aatttctgct tgatctctcc
     2041 gttatcgatg atggaggcgg aat
//
LOCUS       DAORHD010000972         2063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68555_length_2063_cov_3.888944, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000972 DAORHD010000000
VERSION     DAORHD010000972.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68555_length_2063_cov_3.888944"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2051)
                     /locus_tag="PKO06_17645"
     CDS             complement(<1..2051)
                     /locus_tag="PKO06_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71533.1"
                     /translation="MRCESGPVSEQILTFYADTRAVADVQHPMYGDALDHVLEAIVAP
                     RLGIDTLLHDLKTKALRLDSDRLDQLHGLLAAIVSDLRTPVVRELAQLPIWPDRDGSL
                     GCLSGPDSVFIPADPDIAALFPKTRFLHARLLNHPLLEQVKVRRFGAAAVCQAFSETS
                     EDEFRLKATPANVERVQAYLLSHADEVRLLKPERLLKQPLFRSQRGDFAPAEELKIPS
                     DPAILEFARFLPRCRMLDLEDRGYELLKVFGLEKQIAPYQVDHLVEDVVGLWQSAKKK
                     EEWARSFPPLYTLEGIRRFFTLVMASYRSIPQLMLGRLLNLPIFPDEEGVCGILRDKS
                     GWHPRAVFPCVAELRPIFRTLKLRLLDEQIQNLAWPVLQGIPGWAYLDVLVEHPELRK
                     PYTGEGGRKPHQEEAFLCSVIQYLVEHRTEVLREFPPTPRKNLPPANSSLNACCIWPT
                     RAGKVLSAGELFEGETLDLLIDPDSPEDVWCRERMLIDAVRPAFRALQPGFAPADVAE
                     VIDELVGEFAKADEPLANQNPFLSTVQRVAIVFQYLDDRYSDDVNRRDTLPVVDAGGR
                     LVFRALFTADESTLDLVRGLGGEGVIMDPAFKACLPKSVFRQQRFPAYPTSDVILHVA
                     VQPITKIHGGEPPAAGAESGTVPAALEPCRGKFIDWLMEHESDVFQDLVACDLLRRAK
                     I"
BASE COUNT          467 a          636 c          571 g          389 t
ORIGIN      
        1 aagatcttcg ctcgccgaag cagatcacac gccacgagat cctgaaacac gtcactttcg
       61 tgttccatca gccagtcaat gaacttgccg cggcagggct cgagcgctgc cggtaccgtg
      121 ccggattctg cccctgccgc cgggggctcg ccgccgtgaa tcttcgtgat cggttgcacc
      181 gccacatgca gtatgacgtc ggacgtggga tacgcgggaa agcgctgctg acgaaagaca
      241 ctcttgggca gacaggcttt gaaagccgga tccatgatca ctccctcacc cccgagcccg
      301 cgcaccaggt caagcgtcga ctcatctgcg gtgaagagag cgcggaacac cagccgtccg
      361 cccgcgtcaa ccaccggcag ggtgtcgcgc cggttcacgt catcagagta ccggtcatcg
      421 agatactgaa agacgattgc aactcgttga acggttgaga gaaacggatt ctgattggcc
      481 agaggttcat cggcctttgc gaactcgccg accagttcat cgatcacttc cgccacatcc
      541 gccggcgcga aaccgggctg caaagctcga aacgctggcc gaaccgcgtc aatgagcatt
      601 cgttcccggc accagacatc ttccggcgaa tcgggatcga tcaacaggtc gagcgtctcg
      661 ccctcgaaca gttctcccgc cgacagcact ttcccggccc gtgttggcca gatgcagcag
      721 gcattgagcg aactgttggc cggcggcaga tttttgcgcg gggtcggcgg aaactctcgg
      781 aggacctcag tgcggtgctc gaccagatat tgaatgaccg aacagagaaa agcctcttcc
      841 tgatgcggct ttctcccccc ttcgcccgta tacggctttc tcaattccgg gtgttcgacc
      901 agaacgtcca ggtaagccca acctggaata ccctgcagga cgggccaggc aaggttctga
      961 atctgctcgt ccagcaggcg cagcttcagc gtgcgaaaga tcgggcgtag ttcagcaaca
     1021 cagggaaaca ccgctctggg atgccacccc gatttgtcac gcagaatgcc gcagacccct
     1081 tcctcgtcag gaaaaatcgg cagattcagc aggcgaccga gcatcagttg tggaatcgaa
     1141 cgatacgagg ccatgaccaa cgtaaaaaaa cgcctgatcc cttcgagggt gtacaggggc
     1201 ggaaacgatc tggcccattc ttccttcttc ttggccgact gccagagacc cacgacatcc
     1261 tcaaccagat gatcgacctg atagggggca atctgctttt ccaagccgaa caccttcagc
     1321 agctcgtagc cacggtcttc cagatccagc attcgacagc gcggcagaaa gcgggcgaac
     1381 tccagaatcg ccggatcact cggaatcttc agttcctcag cgggagcaaa gtcgcctctc
     1441 tgcgagcgaa acaggggttg cttcaacaag cgttcgggtt tcagcaatcg gacttcatcc
     1501 gcatgactga ggagatacgc ctgcactcgc tccacgttcg ccggagtggc tttgagccga
     1561 aactcatcct cgctggtttc tgaaaaggcc tgacagactg cagccgcacc aaaccgtcgc
     1621 actttcacct gttccagcag gggatggttc agcaaccgtg cgtgcagaaa ccgggtcttc
     1681 ggaaacagtg ctgcaatgtc cgggtcagct ggaatgaaaa cagagtcagg tccactgaga
     1741 caacccagag aaccatcacg atcaggccag attggcaact gggcgagttc tcgaaccacc
     1801 ggtgtgcgca ggtcagatac aatggccgcc agcaacccgt gcaattgatc aagccgatcg
     1861 gagtcgagcc gcagcgcttt cgttttcaga tcgtgcagca gggtatcgat ccccagacgc
     1921 ggtgccacga tcgcttccag cacgtgatcg agagcgtcgc cgtacatcgg atgctgcaca
     1981 tcagcgacgg cacgcgtatc ggcatagaag gtcagtatct gttcactgac cggtcccgac
     2041 tcgcatcgca ccaacttttg cgg
//
LOCUS       DAORHD010000973         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68720_length_2060_cov_2.815461, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000973 DAORHD010000000
VERSION     DAORHD010000973.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68720_length_2060_cov_2.815461"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKO06_17650"
     CDS             <1..468
                     /locus_tag="PKO06_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71534.1"
                     /translation="DSCLKRLGRTLETLPWSELNTLDYILVEYVGENDLGGLTLGDLE
                     KLRRLCRVPLILAHPNIERLTESLPLNLIVEVLRRHHVALEIPAGTRNEWFWNRIDPA
                     PLRRIALTIGTDTHHDLAEVADIERTLAFLTRTGLDKNLATPDTLASASGGAL"
     gene            465..749
                     /locus_tag="PKO06_17655"
     CDS             465..749
                     /locus_tag="PKO06_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV71535.1"
                     /translation="MNKPRDRVVITAMGANRPGVLAGITRVLAEANVNIDDVSQKIMQ
                     EFFALIMVTDFSGANGSFEDIQGRLQKLADELKVKVFVQHESVFTFMHRL"
     gene            763..>2060
                     /locus_tag="PKO06_17660"
     CDS             763..>2060
                     /locus_tag="PKO06_17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HNV71536.1"
                     /translation="MVFTLEEVVETVAAVGVETFDIRTITCGISLHSCADEDVQKCAG
                     KIYDKVMHVAGRLVPTARDIEKRYGIPIVNKRVSVTPIAQIGAACRGDTLVPLAQALD
                     RAADELGIDFIGGFTANVQKGFMRGDQLLINSIPDALCATKRVCSSVNVAGSHAGINM
                     DAVLRMAEIIKEAAERTRDRDGLACAKLVVFANQPEDNPFMAGACHGAGEAEAIVHVG
                     ISGPGVVHAALKQAGPLPFNELSELIKKTSFKITRVGELVGREASRMMGIPFGVIDLS
                     LAPTPAEGDSIADILEAMGLERTGCHGTTAALALLNDAVKKGGVMACTYAGGLSGAFI
                     PVTEDASMCRAAAEGALTIEKLEAMTSVCSVGLDMVAIPGDTSVDQIAGIIADEMAIG
                     VINHKTTACRLIPVPGKKAGEEVDFGGLLGKGPIMAVNRFS"
BASE COUNT          430 a          620 c          585 g          425 t
ORIGIN      
        1 gacagttgcc tgaagagact tgggcgcaca ctcgaaaccc tgccctggtc tgaactgaac
       61 acgctcgact atattctagt cgagtacgtc ggtgaaaacg acctcggcgg cctgaccctc
      121 ggtgacctgg agaaacttcg ccgactctgt cgtgtgccgt tgattctggc acatccaaac
      181 atcgaacggc tcacggaatc gcttcctctg aacctgatcg tcgaagtatt gagacgccac
      241 cacgtagcgc ttgaaatacc ggccggaaca cgcaacgaat ggttctggaa ccgcattgat
      301 cccgctcctc ttcggcgcat cgccttaacc atcgggaccg acacgcatca tgaccttgcc
      361 gaggttgccg acatcgagcg cacgctcgcc tttctcaccc gcactggtct cgacaaaaat
      421 ctcgcgaccc ctgacactct cgcatccgca tctggaggtg ccctgtgaac aaaccccgcg
      481 atcgcgttgt tatcactgcc atgggagcca accgccccgg cgttctcgcc gggattacgc
      541 gcgttctggc cgaagccaac gtcaacatcg atgacgtctc gcagaagatc atgcaggagt
      601 ttttcgccct gatcatggtc acggatttct ccggggccaa tgggtccttc gaagacatcc
      661 agggacggct gcaaaaactc gccgacgagt tgaaggtgaa ggtattcgtc cagcacgaga
      721 gcgttttcac gttcatgcat cgcctctgat cggagttctc acatggtctt cactctcgag
      781 gaagttgtcg aaaccgttgc tgccgtaggc gttgaaacct ttgacattcg cacgatcacc
      841 tgcggtatct cgctgcatag ttgcgccgac gaggatgttc agaaatgcgc cggcaagatc
      901 tatgataaag tcatgcacgt cgcagggagg ctcgtaccca ccgctcgcga cattgaaaaa
      961 cggtatggta ttccaatcgt caacaaacga gtctcggtaa cgccgattgc tcagattgga
     1021 gctgcatgcc gcggcgacac gctcgtgccg ctggctcaag cgctagatcg agcagctgat
     1081 gaactgggaa tcgacttcat cggcggcttt accgccaacg tgcagaaggg cttcatgcgc
     1141 ggcgatcaac tgctgatcaa ctccattccc gatgcgctgt gcgccaccaa gcgggtctgc
     1201 tcgagcgtca acgtggccgg atcacacgcc ggcatcaata tggacgcggt attgcgcatg
     1261 gccgagatca tcaaggaggc cgccgagcgc actcgcgacc gtgacggact cgcctgcgcc
     1321 aagctggtgg tgtttgccaa tcaacccgaa gacaacccct tcatggcagg cgcctgtcac
     1381 ggtgctgggg aggcggaagc catcgttcac gtgggcattt cgggtccggg ggttgtgcat
     1441 gcggcactga agcaggccgg gccgttgcct ttcaacgaac tttccgaact gatcaagaag
     1501 accagcttca aaatcacccg cgttggcgaa ctcgtcggtc gtgaagcctc ccgcatgatg
     1561 ggcattccct ttggcgtcat cgacctgtct ctggcgccaa ccccggccga aggcgattcg
     1621 atcgccgaca ttctcgaagc catggggctc gagcgtactg gttgccacgg caccaccgcc
     1681 gctctcgccc tgttgaatga cgccgtaaaa aagggagggg tcatggcttg cacctatgcc
     1741 ggcggcctct cgggtgcttt cattccggtc acggaagacg ccagtatgtg tcgagccgct
     1801 gccgaaggag cgttgaccat cgagaaactc gaagccatga cctcggtgtg ttccgtcggg
     1861 ctcgatatgg tcgccattcc gggtgacact tccgtggatc agattgccgg gatcattgct
     1921 gacgaaatgg ccatcggcgt gatcaatcac aagacaaccg cctgccgcct gattccggtt
     1981 cccggaaaaa aggccggtga ggaagtcgat ttcggcgggt tgctcgggaa ggggccgatc
     2041 atggccgtga atcggttctc
//
LOCUS       DAORHD010000974         2056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68913_length_2056_cov_3.450275, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000974 DAORHD010000000
VERSION     DAORHD010000974.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68913_length_2056_cov_3.450275"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PKO06_17665"
     CDS             complement(<1..377)
                     /locus_tag="PKO06_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71537.1"
                     /translation="MIGRFSVVAALACLLCAPLWAQSTGSATGSPTPDLPLLSQQAPG
                     DVPPALMPPPPPPPPPSQLPVPAPVQPAPSQLPPGPAPAKTQAEAAPTPAALTLSPSA
                     PVLEPPPPPSGFVLPASAQMTNST"
     gene            511..583
                     /locus_tag="PKO06_17670"
     tRNA            511..583
                     /locus_tag="PKO06_17670"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:544..546,aa:Ala,seq:cgc)
     gene            770..1348
                     /locus_tag="PKO06_17675"
     CDS             770..1348
                     /locus_tag="PKO06_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71538.1"
                     /translation="MEKLILYPLGFVLLLVGIVVAGIGMVFVAPFAGLIRFSLWLLDS
                     WHEPEKLPAEAPSAEQTKSVTGETQPDLFDTYSRFYTTHNEACLTIDERKQVLEVYRR
                     LKRAQESLAEDLTGECRSDLNEACETITITTPFMADLQHLIQEISRHRHATDLRASKT
                     LARLIRQLQYLSAIQESTTLVRQDDRPAHQLC"
     gene            complement(1345..1794)
                     /locus_tag="PKO06_17680"
     CDS             complement(1345..1794)
                     /locus_tag="PKO06_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71539.1"
                     /translation="MAVKIWVPRTLDQLLIKLSDHFHESKTRCLRQILFKYAYGQVQL
                     EKFRSVAIQGAGDPEADPDRPLYSRTSASDPYATPELGKCGEDIKLWIPQKLRDDLQI
                     LADRASVPLSQFIREILVSELLGHVYLPERRSLVEGEGPTEKNPPSA"
BASE COUNT          426 a          600 c          586 g          444 t
ORIGIN      
        1 gtcgagttgg tcatctgcgc cgacgcgggc agaacgaagc cgctgggggg cggcgggggc
       61 tcgagaactg gcgcggatgg cgacagcgtc agggccgcgg gagtcggtgc ggcttcagcc
      121 tgggtcttgg ctggcgccgg accgggcggc agctgactcg gcgccggttg cacgggagcc
      181 ggcacgggaa gctgacttgg cggcggcgga ggcggtggag gtggcatcaa tgcgggcggc
      241 acatcgcccg gcgcctgctg gctcagcaga ggcagatcag gagttggcga ccccgtcgcc
      301 gaccccgtag actgcgccca gagaggagcg cacaacaaac aggccagcgc ggccacaaca
      361 gagaatcgtc cgatcatgaa acctccttgt cacgaacgca gcaaaatgaa cccactgccg
      421 caaagcttac cggtaccgcc cagcagatgc aaatcattcg acaacctgct tgaaagttcc
      481 gtggtcttgc gctataatgg ctcttgttac ggggctgtag ctcagctggg agagcgcgac
      541 aatcgcactg tcgaggccgt cggttcgatc ccgatcagct ccaccgtcac acgaggcaac
      601 gagacatcgt tgcctcgtgt gctttttcac ccgttctatt acacagccaa gttatcacct
      661 atcgacctgt ccagtattgc ccggccagct ctgaaaaggt gcagtaagaa ataggggaat
      721 tttctcacct cgccattgtt ctgcgtatct tcaggaacag gaggtgatta tggaaaaact
      781 catcctgtat ccgttggggt ttgtgctcct gctcgttggc attgtcgtgg cgggcatcgg
      841 tatggtcttt gtggccccgt tcgccggatt gattcgcttt tcgctctggc ttctcgacag
      901 ctggcatgaa ccggagaagt taccggcgga agcgccttct gccgaacaaa cgaaatcggt
      961 gacaggcgag acccagccag acctgtttga tacgtattcc cggttttaca cgacccacaa
     1021 cgaagcgtgt ctcacgatcg acgagaggaa acaggtgctc gaggtgtatc ggcgtctgaa
     1081 acgggctcag gaaagtctgg cggaggacct gaccggtgaa tgccgcagcg atctcaacga
     1141 ggcatgcgag actatcacga tcaccacgcc tttcatggcc gacctccagc atctcattca
     1201 ggagatcagc cgtcatcgcc atgccaccga tcttcgcgcc tcgaaaaccc ttgcccgtct
     1261 gatccgtcag ctgcagtatc tttctgctat ccaggaatca acgactctcg tccggcagga
     1321 cgatcgaccc gcccatcagc tctgttaagc gcttgggggg ttcttttccg tcgggccttc
     1381 gccttcgacc aacgatctcc gttccggcag atacacgtga ccaagcagtt ctgaaaccag
     1441 gatttcccgg atgaactggg acaacggaac actggctcgg tcggcgagga tttgcagatc
     1501 atcacgcagc ttttgcggta tccagagctt gatgtcctct ccgcatttcc ccagctcggg
     1561 tgttgcatac gggtcgctcg cagaggtacg ggaatacagc gggcgatcag gatctgcctc
     1621 cggatcgccc gctccctgaa tggctacgct tctgaatttt tccaattgta cttgtccgta
     1681 cgcgtatttg aacagtattt gtcgcaaaca gcgggtcttg ctttcatgga agtggtcgga
     1741 caatttgatg agcagctgat cgagtgttcg gggcacccag attttgactg ccaccgagtc
     1801 ctgctcgaac tcgctaaagt cggctgccgt tccaccgaac gtctggaaac actcaccttg
     1861 cttgcccggc gcgggtgacc tgaaccagtt cgcaaaaaaa ttcctgaaca tattctctcg
     1921 cctttcgggt gaacgcttgg gtgcccgtga aaccgctttt taccgcacac tctcacaaac
     1981 agacgccggg aatcaactgt tttccgatac agatcacttt cagccgacaa gttcgttgcc
     2041 gcaattcgga cactcg
//
LOCUS       DAORHD010000975         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_68971_length_2055_cov_2.857500, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000975 DAORHD010000000
VERSION     DAORHD010000975.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68971_length_2055_cov_2.857500"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PKO06_17685"
     CDS             complement(<1..1488)
                     /locus_tag="PKO06_17685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71540.1"
                     /translation="MSLPQIPTREAVLRLLHSADETDRKQGIIELIRSHDALSRELLE
                     HIAAEDPSVEIRYFARRALASLDTAAGEVSEAPPPVADHDLTLLERLQSTDHRIRLRT
                     LRECQRLPLSDTLPALCRSLVREGDPRWQARFLTALGRLRTPDAVECILPYLQNRNTR
                     VRANAVEALGSICTRDALKPLVPLLSDDDNRVKANVIQALHRLGSRDLLELLRQLAQD
                     SQIWMRDSAIFALSRFDQPEAREILETISRTDPSDRLRQKALALLQPPSATTSESPAG
                     PAQGLVSESQQPVSSTPSSVSSATLLTEASTPIVPVQTAQPLPPLRHTLWEEPAAPLR
                     ALHLWQRLAAGEDISDLGVLQTGLRRETDELCLTLLISLLRQRPAGELFAELVPHLDS
                     PRTRVRAAAIETLSRLDQARAMTHAYALLEDVNNRVRANAVLVLIAEPLFPALTHLRA
                     MARDPRETWRLSVLYVLHQKPRPELLPLLGELLDDASERVRDAALQ"
     gene            complement(1485..>2055)
                     /locus_tag="PKO06_17690"
     CDS             complement(1485..>2055)
                     /locus_tag="PKO06_17690"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNV71541.1"
                     /translation="GVNLPASPAEPVRKGKGVMGSAPKTTEDRNRVKKALSVTLESYL
                     EEICALQERFGAVRITDLAERMGCRLPTATSAVRRLAKLELINYESYRPVTLTEKGQA
                     TVRELTSRHKVIADFLQNVLAFPGPVSQQEACRLEHRIGSRILRRLRLYMLFLRDRPV
                     AVEALQKRTRKFSSFVRQRARKSGSGRPT"
BASE COUNT          413 a          579 c          679 g          384 t
ORIGIN      
        1 ctgcagcgcg gcatcgcgca cccgctcaga ggcgtcatcg agcagttcac cgagcagagg
       61 cagcagctct ggtcgcggtt tctggtgcag cacgtagagc acgctcaaac gccaggtttc
      121 acgcggatcg cgcgccatgg cgcgcaggtg cgtcagagcg ggaaacaatg gctcggcaat
      181 caggacaaga acagcgttgg cgcgcacccg gttgttgaca tcttcgagca gggcgtatgc
      241 atgtgtcatg gctcgagcct gatcgagcct cgagagggtc tcaatggctg cggcacgaac
      301 ccgcgtacgc ggcgaatcga gatgagggac gagttcggcg aacagttcgc cggccggtcg
      361 ttggcgcagc aacgagatca acagcgtgag acacaactca tcggtttcgc gccgcagacc
      421 agtctgcaga acgccgaggt ccgagatgtc ttctcccgcc gccagacgtt gccatagatg
      481 cagggcgcgc aggggcgccg cgggttcttc ccacagggtg tggcgcaacg gtggcagtgg
      541 ctgcgctgtc tgcacgggaa cgatcggagt cgacgcctcg gtgagaagtg ttgccgatga
      601 aactgagctt ggagtcgacg aaaccggttg ttgtgactcg gacacgagac cttgcgccgg
      661 gccggcgggg ctctcggatg ttgtggcaga tggcggctgc aacagcgcca gcgctttctg
      721 tctcaggcgg tcagagggat ccgtgcggga gatcgtttcg agaatctcgc gagcttcggg
      781 ttgatcaaac cgagacaagg caaaaatcgc ggaatcccgc atccagatct ggctgtcctg
      841 ggcgagttgc cgcaacagct cgagcagatc gcgactgccg agacgatgca gagcttgaat
      901 gacgttggcc ttgactcggt tgtcgtcgtc actcaagaga gggactaacg gtttcaaggc
      961 atcgcgagtg cagattgagc ccagagcttc gactgcattg gctcggacac gcgtgttgcg
     1021 attctgcaga tagggcagaa tgcactcgac ggcatcaggc gtgcgcagtc ggccgagtgc
     1081 cgtgagaaaa cgggcctgcc agcgcgggtc tccttccctg accaggctac gacagagcgc
     1141 cggcaatgtg tcagacagcg gcaggcgttg gcactcgcgc agggtgcgca gtcgaatgcg
     1201 atggtcggtc gattgcagtc gttcgagcag tgtcaggtca tggtcagcga cagggggcgg
     1261 cgcttctgaa acctcgccgg cggcggtgtc gagcgaggca agcgcccggc gtgcgaaata
     1321 acgaatttca acactgggat cttcagcggc aatgtgctcg agcagttcgc gcgaaagtgc
     1381 atcatgagaa cgaatcagct cgatgatgcc ctgcttgcga tctgtttcgt ctgccgaatg
     1441 cagcagccgc aagacagctt cgcgagtggg aatctggggc aggctcaagt cggtcttccc
     1501 gaaccggatt tcctcgcgcg ctgtcgcacg aaagagctga acttgcgggt gcgcttctgc
     1561 aaggcttcga ctgccacggg gcggtcgcga agaaacagca tatacaggcg caggcgccgc
     1621 agaatgcgcg atccgatgcg atgctcgaga cgacaggctt cctgctgaga gacggggccg
     1681 ggaaaggcga gtacattctg cagaaagtcc gcgataacct tgtggcgtga agtcagctca
     1741 cgtacggttg cctggccctt ctccgtcaac gtgaccgggc gatatgattc gtagttgatc
     1801 agctcgagct tcgccagacg ccgcaccgcg ctcgtcgcgg tcggcagccg acaacccatg
     1861 cgctcggcga ggtcagtgat gcgtaccgca ccgaaccgtt cctgaagggc acagatttcc
     1921 tcaagatagg actcgagggt gactgacagc gccttcttga cacggttcct gtcttcagtt
     1981 gtttttggtg cagaccccat gacacccttg ccttttcgga caggttcggc aggacttgcc
     2041 ggtaaattga cccca
//
LOCUS       DAORHD010000976         2054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69010_length_2054_cov_3.637319, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000976 DAORHD010000000
VERSION     DAORHD010000976.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69010_length_2054_cov_3.637319"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKO06_17695"
     CDS             complement(<1..206)
                     /locus_tag="PKO06_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71542.1"
                     /translation="MDSWQNFVLNLAEESRLDERPQRSPARFAAADAEPFETGAIQIL
                     HLTIKGRQRDDFGAVLNDGCQLLQL"
     gene            192..>2054
                     /locus_tag="PKO06_17700"
     CDS             192..>2054
                     /locus_tag="PKO06_17700"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71543.1"
                     /translation="MPGIHETGSWEGELQYCNPVQENVLEMHTLCFIVRDPNTGEPQY
                     LAHVGLDITARKHQERLRAVREQRVVRLNEALVALARNKLLFRGDLEKSFRLLTETAG
                     LAMDVDRLGVWLLQPNRNALECLDLYDRKAGKHEKGMVLDESQYPEYLRTVNLDSLVF
                     ADDVLRDPRWAHLVATYLKPLGIRSLMFAPIQVGGRTIGLVSHANQRESHSWSAEEQS
                     FAMAVAGLVGTIVDANERRVMESALRLARDELERRVEQRTEQLARANEQLREAQVELE
                     HRVEARTEELSHTNESLKLLLAEQTVNVELANRMLAMIHPPLKRYSTLAGGAQLFVTG
                     RYVPCHAAGGDHYFMRRVMRGSEQRPFSHISLKDQSGHEVGCVLRCTMTDLIHLALLQ
                     DQTLSGVEQVMSRLNDEVCSSGFFGEDGFFTSINLDIDHRTLETRFVSTGHPPFLLIR
                     GSEVLRIPDSQQPGANLPAGMLPGQRYSAGTMRLEPGDRVLLYSDGLFELPRLQGNPA
                     SGSTELQQLVVSMLRDSPEMTVSDLIAAVFERFGLFEREGHPFPVLTDDVALVGLEVE
                     PASAWIETVLQPSDHDHFSRLSEELYETVAREWQEHGFVMPEFRLRLALEESLLN"
BASE COUNT          404 a          559 c          638 g          453 t
ORIGIN      
        1 agttgcagaa gctggcagcc gtcgttcaga actgctccga agtcatcgcg ttggcgaccc
       61 ttgatggtca ggtggagtat ctgaatcgct ccggtctcga aaggctcggc gtcagcagcg
      121 gcgaagcgcg ccggcgatcg ttgtggtcgt tcgtccaacc ggctttcctc ggcaagattc
      181 aaaacgaagt tttgccagga atccacgaaa ccggctcctg ggaaggcgaa ctgcagtact
      241 gtaacccggt tcaggaaaac gtgctcgaga tgcatacgct ctgcttcatc gttcgtgacc
      301 cgaataccgg tgaaccgcag tatctggcgc atgtcggcct cgacattacc gcgcgcaagc
      361 atcaggagcg cttacgtgcc gttcgagaac aacgggtggt gcggttgaat gaagcgctgg
      421 ttgctctggc acgtaacaag ctgctctttc gcggtgatct cgaaaagagt tttcgactct
      481 tgacggaaac cgccggtctg gccatggatg tggaccggct cggcgtgtgg ctgctgcagc
      541 cgaatcgaaa tgccctcgag tgtctcgacc tttacgaccg caaggccggc aagcacgaaa
      601 agggaatggt cctcgatgaa agtcagtatc cggagtattt gagaaccgtg aatctcgatt
      661 cgctggtgtt cgccgacgat gtgctccgtg acccgcgatg ggcccatctc gtcgcgacct
      721 atttgaaacc gcttggcata cgttctctca tgttcgcgcc gattcaggtc gggggtcgca
      781 cgatcgggtt ggtttcgcat gccaaccaac gagaaagtca ttcctggagc gcggaagaac
      841 agagttttgc catggctgta gccgggttgg tcggcacgat cgttgatgcc aacgaacgtc
      901 gagtcatgga atccgccttg cgtctggcgc gcgacgaact cgagcggcgg gtcgaacagc
      961 gtaccgagca gttggccagg gcgaacgagc agttacggga agcgcaggtc gagcttgaac
     1021 accgagtcga ggctcgcacc gaagaattgt cgcataccaa tgagtctctg aagctgctgc
     1081 tggcggaaca gacggtgaat gtcgaactgg ccaaccggat gctcgccatg attcacccgc
     1141 ctctgaagcg atattccaca cttgccggtg gggcacaatt gttcgtcacg ggccggtatg
     1201 tcccttgcca tgcagccgga ggtgatcact atttcatgag gcgagtgatg cgtgggtcgg
     1261 aacaacgacc gttcagccat atcagcctga aagaccagtc ggggcatgag gtcgggtgtg
     1321 tgctgcgctg taccatgact gatctgatcc atctcgcctt gctacaggac cagaccttgt
     1381 ctggggtaga acaggtcatg agccgcctca acgatgaagt ctgttcttcc ggatttttcg
     1441 gcgaagacgg cttcttcacg tcgatcaatc tcgatatcga tcatcgcacc ctcgagacgc
     1501 gctttgtctc gaccggtcat ccgccctttc tgctgatccg tggttccgag gtgttgcgga
     1561 tccctgacag tcagcaacct ggcgcgaacc tgccggccgg gatgctgccg gggcagcgct
     1621 attccgcggg aacgatgcgt cttgagcccg gggatcgcgt tcttctctat tctgatggcc
     1681 tgttcgagtt gccgagattg cagggaaacc cggcctctgg ttccacggag ttgcagcagc
     1741 tggtggtgtc gatgctgcgg gattctcccg aaatgaccgt gtcagatctg atcgcggcag
     1801 tattcgagcg tttcggtctc ttcgaacgcg aggggcaccc tttcccggtg ctgaccgatg
     1861 atgtggcgtt ggtcgggctc gaggtcgagc cggcgtcagc ctggatcgag acggttctgc
     1921 agccgtcgga ccacgaccat ttcagccgat tgagcgagga actgtacgaa accgtggcca
     1981 gggaatggca ggaacatggt tttgtcatgc ctgagttccg attgcgactg gcgctggagg
     2041 agagtctgct gaat
//
LOCUS       DAORHD010000977         2053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69076_length_2053_cov_3.006507, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000977 DAORHD010000000
VERSION     DAORHD010000977.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69076_length_2053_cov_3.006507"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PKO06_17705"
     CDS             <1..223
                     /locus_tag="PKO06_17705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71544.1"
                     /translation="GEEKAFKAAPAAEKQPAASPAATAAKGKPGKDEAAPAGPVIPTR
                     AKVTLAIWAIAISLWLIILTIIAMRFFTG"
     gene            471..>2053
                     /locus_tag="PKO06_17710"
     CDS             471..>2053
                     /locus_tag="PKO06_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNV71545.1"
                     /translation="MTENASRERQESFGSRVLSSRLFLIYTLITFAGLGLLVALTVRA
                     LTRQAEIETDISRHGAMQRASKTGLAFARSLIDELPRTPALLLKAPKLPPTPTVFSRL
                     EQSAVFLRLAAADHSERLNLILEEAVSASESEKPFWWLLAVLHQVDLDPSVADLWAQR
                     LLAGGRDWSITGQPSAFTLASLHLARSFARRGSLGSASAWLQRTAQGAPLPHKFDLRH
                     FWGTAPVPAGWADLFALADLLHRSPRPYEPYRGWHLSKEEPKWPSGPRAQEQYLLDFV
                     GSHTAWIVVESDSRTYAVEARHRFSDLYKQILADGDPGFVKTLSWSWDGTQESAALAG
                     DPGLFLTLRKLPSDALHTSRRYALLVAGVALTLGGLLSMLLLFTRREQQAEMRATRED
                     LYRQAAHDLKTPLTTLRALAETLALRRTRDAEQQERYLQALVREVDQAERVVDGMLLA
                     ARLEAGSVEPRLEAVALEPLVADLLKSFSPRLEGWKIETHFATVPVLFADVSLMKRVL
                     ANLIENVIRHAYQGRELRI"
BASE COUNT          371 a          690 c          600 g          392 t
ORIGIN      
        1 cggcgaggaa aaagccttca aggcagcgcc agccgccgag aaacagcccg ccgcctcacc
       61 cgccgccacg gccgcgaaag gcaagccggg caaagacgag gccgctcccg ccggcccggt
      121 cattcccacc cgcgccaagg tcacgctggc catctgggcg atcgccattt cgctctggct
      181 gatcattctc accatcatcg ccatgcgttt cttcaccggg tgatcccatc gggttttcct
      241 ttctgcattc atgaaatgtc acttgcggag tgtcctagac aggaacgagt ccccggaggt
      301 tctccgcgtc agcggcaggg ccatggtttt cggtggcctc ggttttcaag ccgggcggct
      361 tgagtccggc ggtcaaggtc cgagtccggt ctttccaacc aggggcatcg accgcagtgg
      421 tttcgagaga atccacacgc gtctttgatg acgaaccggg acacacccac gtgacagaaa
      481 acgcctcccg cgaacggcaa gagagcttcg gatcacgggt tctttcatct cgactgtttc
      541 tgatctacac gctgatcacc ttcgccggtc tcgggttgct ggtcgcgctg accgtgcgcg
      601 cgctcacccg ccaggccgaa atcgaaaccg atatcagtcg acatggggcc atgcaacgcg
      661 cctcgaagac agggctcgcg tttgcccgct cgctgattga cgaactgccg cgaacccccg
      721 cccttctgct caaagcgccc aagctaccgc caaccccgac cgtgttttcc cggctcgaac
      781 agtctgcggt attcctgcgc ctcgcggccg ccgatcactc ggaacgcctc aacctgatcc
      841 tggaggaagc ggtcagcgcc tctgaaagcg agaaaccctt ctggtggctt ctggcggtat
      901 tgcatcaagt cgatctcgac ccgtcagtcg ccgatttatg ggcgcaacgc ctgctcgccg
      961 gcggccgcga ctggtcaatc accggccagc cgagcgcatt cactctggca tccctgcacc
     1021 tcgcccgcag ttttgcccga cgcggaagtc tgggcagcgc ctctgcctgg cttcaacgga
     1081 ccgcacaagg cgctcctctg ccgcacaagt tcgatttgag acacttctgg ggaacagctc
     1141 ccgtccccgc cgggtgggcg gatttgtttg ccctggccga tctgctgcac cgaagcccga
     1201 ggccgtatga accctatcgc gggtggcacc tgagcaagga agagcccaag tggccaagcg
     1261 gcccacgagc gcaggagcaa taccttctcg acttcgtcgg ttcgcacacc gcctggatcg
     1321 tcgtcgaatc cgacagccgc acctatgccg ttgaagctcg ccacaggttt tccgacttgt
     1381 acaaacaaat tctcgcagac ggcgaccccg ggtttgtgaa aaccctgtcg tggtcatggg
     1441 acgggacgca ggagagcgct gctctggccg gggaccccgg actgtttctc actctgcgca
     1501 aactcccgtc tgacgctctg cataccagcc gtcgctacgc attactcgtt gccggtgtgg
     1561 ctttgactct tggtggactc ctttccatgt tgctgctgtt cacgcggcgc gagcagcagg
     1621 ccgaaatgcg cgccactcgc gaagacctgt atcggcaagc cgcgcacgac ctgaagaccc
     1681 cgctgaccac tctgcgagcg ctggccgaga cgctggccct gcgtcgcaca cgcgacgccg
     1741 aacaacagga gcgctatctg caggcgctgg tgcgcgaggt cgatcaggcc gagcgtgtcg
     1801 tcgacgggat gttgctggct gcccgcctcg aagcgggttc ggtcgagcca cgcctcgagg
     1861 cggtcgcgct cgaaccgctg gtcgccgatc ttctgaagtc gttcagtccc cgactcgaag
     1921 gctggaagat cgagacccac ttcgcgaccg taccagtgct cttcgcggat gtctcgctca
     1981 tgaagagggt tctggcgaat ctcatcgaga acgtgattcg acatgcgtat caggggcgcg
     2041 aactgcgcat cga
//
LOCUS       DAORHD010000978         2049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69241_length_2049_cov_4.163992, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000978 DAORHD010000000
VERSION     DAORHD010000978.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69241_length_2049_cov_4.163992"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1398)
                     /locus_tag="PKO06_17715"
     CDS             complement(100..1398)
                     /locus_tag="PKO06_17715"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV71546.1"
                     /translation="MTTKSTPVAVVREQLKNPVTDNVCACLFEEDTHHIRFSNNDINR
                     ENLRKALTTLNVKVGHQGRLGNFQTNDLSEPGLKQAFARAHENATLLPEDPEYMPAVT
                     KDEASLAPLYKLEAKPGTNQDRYALLARIFKELDRHGLTSAGSLVSSTVRATTLNNLG
                     VELAHEEHSVALNVTAMSKSSSFKSTFLDKDLANFDEAAFLRSLVEKTKLSESPQSQE
                     PGRYTVVLSPQAVGELMMFLMWYNFDRKAIDEKRTAMFPRLGKTIADERIHIHVDPRH
                     PACPAFPFSPTDGRPAGKFDMIRHGKFVDGWYTRFYEKKMNLTEKGRWPSNIVFEGTN
                     LSEDELVKNVKDGLFVNGFWYIRLVDLMDGIFTGMTRDGLFRIQNGKLTGSVTNFRFN
                     QNVFEMLMNTVSIGKAFRTDYGVFPFLTVKDFNFISRTEF"
     gene            complement(1398..>2049)
                     /locus_tag="PKO06_17720"
     CDS             complement(1398..>2049)
                     /locus_tag="PKO06_17720"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV71547.1"
                     /translation="SCAGRSFATLEKYQKFRYGSDLVNFVADNTLERGLATYGYDDEG
                     VECQKWHIVKDGIFVGYGVNREVAGMIGLKRANGTTRATRYCDVPIVRIPNLYLAPGT
                     RPLSKQELIADTKKGIYIEGMGSFSIDQMRLNMQFGGDAFWEIKDGKIVGMLKNVTYN
                     AISYEFWRSCDAICDEREFKRHGFLTCGKGDPMQLAQMTHGASPARFRNIMVRRAK"
BASE COUNT          385 a          534 c          663 g          467 t
ORIGIN      
        1 gtcgcggaaa agagcgcgta gcgctgattc gcaagaagtc ccctgccctg ccgctgaatg
       61 ttcgatgaat ccggaggcgg ggcaatgaac cggcgggagt tagaactcgg tgcggctgat
      121 gaagttgaag tccttgacgg tgagaaacgg gaagacgccg tagtcggtcc ggaaggcctt
      181 gccgatcgag acggtgttca tcagcatctc gaagacgttc tggttgaagc ggaagttcgt
      241 cacactgccg gtcagctttc cgttctggat gcggaacagg ccgtcacggg tcatgccggt
      301 gaagataccg tccatgaggt cgacgaggcg aatgtaccag aagccgttga cgaagagacc
      361 gtctttgaca ttctttacga gttcgtcttc ggaaagattc gtgccttcga agacgatgtt
      421 cgaaggccat cgacctttct cggtcaggtt catcttcttc tcgtagaagc gggtgtacca
      481 gccatcgacg aacttgccat gtcggatcat gtcgaacttg ccggccgggc gtccgtccgt
      541 gggcgagaag ggaaaggcgg ggcaggccgg gtggcgcggg tcgacgtgga tgtgaatgcg
      601 ctcgtcggcg atcgtcttgc cgagacgggg aaacatggcg gtacgtttct cgtcaatcgc
      661 tttgcgatcg aagttatacc acatcaggaa catcatcagt tcgccgacgg cctgcggtga
      721 gagtactacc gtgtaacggc cgggttcctg tgattgtggg ctttccgaga gcttcgtttt
      781 ctcgacgagc gaacgcagga acgccgcttc gtcaaagttc gccagatctt tgtcgaggaa
      841 ggtgctcttg aacgaagatg atttgctcat cgcggtgacg ttcagagcga cggagtgttc
      901 ctcgtgggcc agctccacac ccagattgtt gagcgtcgtg gctcggacgg ttgaagacac
      961 cagggatccg gcggaggtga ggccgtgacg gtcgagttcc ttgaagatgc gggcgagaag
     1021 ggcgtagcgg tcctggttgg tgccgggctt cgcttcgagc ttgtacagcg gagcgagcga
     1081 ggcctcgtcc tttgtgaccg cgggcatgta ttcagggtct tcgggcagaa gcgtcgcgtt
     1141 ctcatgggcc cgtgcaaatg cctgtttcag gccgggttca gaaaggtcgt tggtctgaaa
     1201 gttgccgagg cgcccctgat gcccgacctt gacgttcaag gtcgtgagcg ctttccgcag
     1261 attttcgcga ttgatgtcgt tgttcgaaaa cctgatgtga tgcgtatctt cttcgaacag
     1321 gcacgcacac acgttgtcgg tgacggggtt cttgagttgt tcgcgaacaa ccgcgaccgg
     1381 agtggatttc gtggtcatta cttggccctc ctcaccatga tgttgcggaa acgggccggg
     1441 gaggcaccgt gagtcatctg cgccaattgc atcgggtcgc ccttgccgca ggtgagaaag
     1501 ccgtggcgct tgaattcccg ctcgtcgcag atcgcatcgc agctgcgcca gaactcgtag
     1561 gaaatcgcat tgtaggtcac gtttttcagc atacccacaa ttttgccgtc cttgatttcc
     1621 cagaaggcgt cgccgccgaa ctgcatgttg agccgcatct gatcgatgct gaacgagccc
     1681 atgccctcga tgtagatacc cttcttcgtg tcggcgatca gttcctgctt tgacagcggt
     1741 cgggtgccgg gtgcgagata gagattggga atgcgcacga tcggcacgtc gcagtagcgc
     1801 gtggcgcggg tggtgccgtt ggcgcgcttg aggccgatca tgccggcgac ttcgcgattc
     1861 acaccgtagc cgacgaagat gccgtctttg acgatgtgcc acttctggca ttcgactcct
     1921 tcgtcgtcgt agccgtaggt ggcgaggccg cgttcgagcg tgttgtcggc gacgaagttg
     1981 acgagatctg aaccgtagcg gaacttctga tacttctcga gggtcgcgaa actgcgcccg
     2041 gcacagctt
//
LOCUS       DAORHD010000979         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69281_length_2048_cov_3.916708, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000979 DAORHD010000000
VERSION     DAORHD010000979.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69281_length_2048_cov_3.916708"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..714)
                     /locus_tag="PKO06_17725"
     CDS             complement(136..714)
                     /locus_tag="PKO06_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNV71548.1"
                     /translation="MMTTNILLCGVGGQGTLLASNVLAETAMEAGFDVKKSEVHGMAQ
                     RGGSVVSHVRFGKQVQSPLIRKGECQILLAFEQLEALRWSEYLQPNGRILMNVQKIMP
                     MTVSAGSDKYPEEIKPQLEKLPAQVTAVDALAEAQKLGNAKCVNVVLLGLLAKSMPDL
                     AKDLWLSVLQRRLPPKLVELNLKAFDAGYALG"
     gene            complement(954..>1982)
                     /locus_tag="PKO06_17730"
     CDS             complement(954..>1982)
                     /locus_tag="PKO06_17730"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV71549.1"
                     /translation="KKKNAAFLKLGLPHPFPRELVKKFAGMVKTLIVVEENDPFMEEQ
                     VRALGLACVGKDRIPICGELNPDILSQSLKDFGAAGAPVRVTMPETAVPMRPPVLCPG
                     CPHRGVFFLLRKLNVDVTGDIGCYSLGALAPHNAMHTIVCMGASITNSLGIRKAMGDK
                     AKGKLVAVLGESTFMHSGITGLLDTVYNQGAHLTMVLDNSITAMTGHQPNPASGLTLK
                     GKPTKAVNLADICRSVGVDFVREVDPFDLVTLEKTMKEALALEEPTVIITKRPCILLK
                     NYKPKPRQLELNRDKCVKCGQCWKMGCPAIERDPADGKTRINFTLCMRCGLCAAVCRP
                     GALTMKDE"
BASE COUNT          394 a          620 c          545 g          423 t
ORIGIN      
        1 gggcgcgcca ggccagcacc gccttgcctt cttccggagc agatgcgggc gaatctgcct
       61 gcagcagggc cacaccccag aaagcgagca ggcagatcac ccacgcgagt cggctcaacc
      121 catctcgccg ccttctcatc cgagggcgta tccagcgtcg aacgctttca gattcagttc
      181 gacgagtttc ggcggcagac gccgctgcaa cacgctcaac cagagatctt tcgccagatc
      241 cggcatcgac ttcgccagca gaccgagcag aaccacgttc acgcactttg cgtttcccag
      301 cttctgagcc tcggccaggg catcgacggc cgtcacctgc gccggcagtt tctcgagttg
      361 cggcttgatc tcttcgggat atttgtccga gccggcggaa accgtcatcg gcatgatttt
      421 ctgcacattc atgagaatgc gaccgttcgg ctgcagatac tcggaccagc gcagggcttc
      481 aagctgttcg aacgccagca gaatttgaca ctcgcctttg cggatcagag gcgattgaac
      541 ctgttttcca aatcggacat ggctgaccac gctgccaccg cgctgggcca tgccgtgcac
      601 ctcggacttt ttcacatcaa acccggcctc catggccgtc tccgccagca cgttgctggc
      661 aaggagcgtt ccctgcccac caacgccgca caacagaata ttcgtcgtca tgatcttgct
      721 cctgtcacaa gagttcaacc gcaagcggca ccgcgccaga ccgtgtggtt ttgcgccgtg
      781 aagctccgct tccggttttg acccgcccgc cacctgtcgt cttgctcgtg tcaactcgat
      841 gagtcacctc gttcgactct ggcgtcgttg tacaactacg cagagatttg aacacgcttt
      901 ctatctgact gtcaggtcta tcaataacgg aagtgcgggt cgttcccatg gaattactcg
      961 tccttcatgg tcagcgcccc ggggcggcag accgcggcac acaacccgca acgcatgcac
     1021 agggtgaagt tgatgcgggt cttgccgtca gcagggtcgc gttcgatcgc cgggcagccc
     1081 atcttccagc attgaccgca cttcacgcac ttgtcacgat tgagttccag ctggcgcggt
     1141 ttcggcttat agtttttcag cagaatgcag gggcgcttgg taatgatgac ggtcggctcc
     1201 tcgagggcca gagcttcctt catcgtcttc tcgagcgtga ccaggtcgaa cggatcgacc
     1261 tcgcggacaa aatcgactcc gaccgaccga cagatgtcgg ccaggttgac cgccttggtc
     1321 ggcttgccct tgagggtcag ccccgacgcc gggttcggct gatgcccggt catggcggtg
     1381 atcgagttgt cgagcaccat cgtcagatgc gccccttgat tgtagacggt atcaagcaga
     1441 ccggtgatgc cggaatgcat gaaagtcgac tctcccagca cggcgaccag tttgcccttc
     1501 gccttgtctc ccatcgcctt gcgaatgccc agcgagttgg tgatcgaagc gcccatgcag
     1561 acaatcgtat gcatggcatt gtgcggtgcc agcgcgccaa ggctgtagca gccaatatca
     1621 ccagtgacat cgacattcag tttgcgcagc agaaagaaca cgccgcgatg aggacagcca
     1681 ggacaaagca ccggcggcct catgggaacg gcggtttccg gcatagtaac tcgtactggc
     1741 gcaccagcgg ccccgaaatc cttgagtgac tggcttaaaa tgtccgggtt gagttcaccg
     1801 cagatcggta tacgatcttt gccgacgcag gctaaaccga gagccctcac ctgttcttcc
     1861 atgaaggggt cattttcctc gaccacgatc agggtcttga ccattcccgc gaacttcttg
     1921 accagctcgc gcggaaacgg atgcgggagc cccagtttca aaaaggcggc attttttttt
     1981 tt
//
LOCUS       DAORHD010000980         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69521_length_2044_cov_3.349422, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000980 DAORHD010000000
VERSION     DAORHD010000980.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69521_length_2044_cov_3.349422"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="PKO06_17735"
     CDS             complement(<1..1246)
                     /locus_tag="PKO06_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71550.1"
                     /translation="MVTEPERRETDSTTAPLVLDIESSAERLTVLGMLLSGSGAAIWI
                     GGAVSGNADIMPGWPHALGVWLGLFFVSAFIRGCLNDRYVLDPGRQVLEWHTQRFVRQ
                     REWIASFSDIVGVCIMRSYNWDNDDSNTIDAALYIYLRGRGFVRVSSWMLSDFSTQRS
                     LSFETLDRLAVQVADLIDCPYWDRDLKHVLACQTVGEAWKACQEAPRNTGYPVPESAF
                     WEWAMKNRGLNPDGDGSVTFSELRDVAVLFMAPGMVSLSLLGVTFFGYGTMVTLARIG
                     MAATLFSFCVAGFVCWLIFLDRFLVPVLPIRTRFDLQRGLISQGSSIFGYELNQRSQP
                     LGRGLALDLRQGWKHKGTRQWHMVLVPEKPDWIPFTVFHCRHDDQVAPRKKVDHLAFL
                     LGVSAIARETNYPETNLPSSTSL"
     gene            complement(1349..>2044)
                     /locus_tag="PKO06_17740"
     CDS             complement(1349..>2044)
                     /locus_tag="PKO06_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71551.1"
                     /translation="WEHRIDLPENLMSQGLLFVRRTLSLSGKLEIEREELKGNSYYVR
                     VWLPKTFWGTKGALTVNLEALNQAPCTTPPAAPTELKIARNSDPMAPTFTWKGLGKFS
                     AVTLYDVNTNQTVWERIAVNALQSEVSGSLKIGHHYKWAVMQSDECAKYSTETQAGFR
                     LETRWETCITCRGTGWVTCNACHGAGGTYSGGANGQPPVYHPCSFCHGTGRERCNICL
                     GSGRVERPIIIVE"
BASE COUNT          464 a          592 c          586 g          402 t
ORIGIN      
        1 tcagcgacgt cgaggacggc aggttcgttt ccgggtagtt ggtttcacgt gctatcgccg
       61 agaccccgag aagaaaggcc agatggtcaa ccttttttcg tggcgcgact tgatcatcgt
      121 ggcgacaatg aaacactgta aagggtatcc agtcgggctt ttccggaacc aggaccatgt
      181 gccactgtcg ggtccccttg tgtttccagc cctgtctgag atcgagcgcc agaccccgcc
      241 ctaacggctg actccgctgg ttgagctcgt aaccgaatat cgagcttccc tgcgaaatca
      301 agcctctctg cagatcgaat cttgtccgaa tcggaagcac gggcaccaag aaacgatcga
      361 gaaagatgag ccagcacacg aagcccgcaa cacagaagga aaacagagtc gcggccatac
      421 caatccttgc cagggtcacc atcgttccat atccgaagaa cgtgaccccc agtaaggata
      481 atgacaccat gccaggagcc ataaacagca cagccacgtc tcgcaattca gaaaatgtga
      541 cggaaccatc cccatccggg ttcagccccc ggtttttcat ggcccattcc cagaaggccg
      601 attcaggaac cggatacccg gtattacgcg gagcttcctg acaggctttc caggcctcgc
      661 caaccgtctg gcaggcaaga acatgcttga gatcacgatc ccaataggga caatcaatca
      721 gatccgcgac ctgcaccgcc agacgatcga gcgtttcgaa cgagagcgat cgctgggtgg
      781 agaagtcgga gagcatccag gacgagacgc gtacgaaccc ccgaccccga agatagatgt
      841 acaaagcggc atctattgtg ttactgtcgt cattgtccca gttatatgaa cgcatgatgc
      901 agacgccgac gatatcagag aatgatgcga tccactcgcg ctgccggacg aatcgttgtg
      961 tgtgccattc gagcacctgc cggccgggat ccaggacata gcgatcgttg agacaacccc
     1021 gaatgaaggc cgagacgaaa aacagcccga gccacacgcc cagtgcatga ggccatccgg
     1081 gcatgatatc ggcattcccg ctgacggctc ccccgatcca gatagcagcg ccggagccgc
     1141 tcagcaacat gccaagcacg gtcagtcgct ccgccgaact ctcgatgtcg agaaccagag
     1201 gtgccgtcgt cgaatcagtc tctcgtcgtt cgggttcagt caccatcgcg aaacagtgta
     1261 gcagctgacg gggtcacgcg aaagttggaa caaagaaaaa agcgctctgc ctgagtctct
     1321 cgggcagagc gcttttcgct gttgatcgtt attcgacgat gatgatcgga cgctcgacgc
     1381 gaccgctgcc gaggcagatg ttgcagcgtt cgcggccggt gccgtgacag aagctgcacg
     1441 gatgatagac cggaggctga ccgttcgccc cgcccgagta agttccgccg gcaccgtgac
     1501 aggcgttaca ggtcacccaa ccggtgcccc ggcaagtgat gcaggtttcc cagcgggttt
     1561 cgagccggaa cccggcctgt gtttcggtgg agtatttggc gcattcgtca ctctgcatca
     1621 cagcccattt gtagtggtgg ccgattttca acgagccgga aacctcgctc tggagagcgt
     1681 tgaccgcgat gcgttcccag actgtctgat tggtattgac gtcatacagg gtaaccgcgg
     1741 aaaactttcc gaggcctttc caggtgaaag tcggggccat gggatcgcta ttgcgggcga
     1801 ttttcagctc ggtcggtgcg gcgggcggag tcgtgcacgg ggcctgattg agtgcttcga
     1861 ggttgaccgt cagagcacct ttggtgcccc agaaggtctt cggcagccac acgcgaacgt
     1921 agtaggagtt ccctttcagt tcttcgcgct cgatttcgag ttttccggaa agcgacaggg
     1981 tgcggcggac aaagagcagc ccctgactca tcaggttttc cggcagatcg atgcggtgtt
     2041 ccca
//
LOCUS       DAORHD010000981         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69537_length_2044_cov_2.523379, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000981 DAORHD010000000
VERSION     DAORHD010000981.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69537_length_2044_cov_2.523379"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..1453)
                     /locus_tag="PKO06_17745"
     CDS             complement(29..1453)
                     /locus_tag="PKO06_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNV71552.1"
                     /translation="MNIPNRHVFKEYDIRGVVDRDLPAPFVQAIGRAFGTFVRRHTGK
                     SQPHLALGQDVRLSSPSLHDAIVKGLLETGVNVTELGPCPTPVVYFSLFHLAVDGAVQ
                     ITGSHNPSEFNGFKLCLGKTTIFGADIQELRRLIEKDEYAYGTGTVDSYDILQAYREH
                     QQKRHAVLKESIRPVKVVIDGGNGTAGLVFPQLMRDLGCEVEELYCTVDGRFPNHHPD
                     PTIPENLAELVRRVKSSGADLGLAFDGDSDRLGLVDETGRILWGDEIMILLARDIVRR
                     HPGAAIIGEVKCSQRMYDAIEQAGARGIMWKTGHSLIKNKMKEEHALLAGEMSGHLFF
                     AEEYFGFDDAMHAALELTRILADHLRQGGRGLSELLSDIPPAIATPEIRTDCADERKA
                     QVVADVRAALEAHLHTGAAPKVRHLITIDGVRAVFEQGWGLVRSSNTQPILVSRFEAV
                     SAEQLAHYRQFMEAIIHDAVRRNA"
     gene            complement(1450..>2044)
                     /locus_tag="PKO06_17750"
     CDS             complement(1450..>2044)
                     /locus_tag="PKO06_17750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV71553.1"
                     /translation="TEAGVPSLIFVITDMIGKPGYLNAEEIRSLASEGFGIGLHGKRH
                     YEVTKILAEGGNLTDELVQARQVLQQVSGKQVTWYAYPFGEYSDAAKLAVASAGLSLA
                     FTIDALEHKRDQDPFKLPRLMYLKGAKEAGEPTVDDWLPPPNIKAGYLHLAMAALVLF
                     FGIQILVRSLRFALYLRQLKLADSSSSTPPTVQGTPT"
BASE COUNT          435 a          574 c          598 g          437 t
ORIGIN      
        1 tcactgggtt cagatgtccg gtgcgggttc atgcgtttct cctgaccgcg tcgtgaatga
       61 tcgcctccat gaattgtcgg tagtgagcaa gttgctctgc cgagaccgcc tcgaagcgtg
      121 agacgagaat cggttgcgtg ttcgaagaac gcacaagacc ccacccctgc tcgaagactg
      181 cgcgaacgcc gtcgatcgta atcagatggc gaaccttcgg ggcagcgccg gtgtgaaggt
      241 gtgcttcaag tgccgctcgc acgtcggcca ccacctgggc ctttcgctca tcggcacagt
      301 ccgtgcgaat ctcgggagtg gcaatcgcag ggggtatgtc gctcagaagt tcagagaggc
      361 cgcgcccgcc ctgacgcaga tggtcggcca gaatccgtgt cagttccagg gcggcatgca
      421 tggcgtcatc aaagccgaaa tactcttcgg cgaaaaacag atgaccgctc atctcacctg
      481 ccagcagtgc gtgttcctct ttcatcttgt tcttgatcag tgaatgaccg gtcttccaca
      541 tgataccgcg ggcgccggcc tgctcgatgg cgtcatacat gcgctggctg catttcacct
      601 cgccgatgat ggctgcaccc ggatgtcggc gcacaatgtc gcgcgccagc agaatcatga
      661 tctcgtcacc ccagagaatg cgaccggttt cgtcaaccag accaagacga tctgaatcgc
      721 cgtcgaatgc gagacccaga tccgcgccag aggactttac ccgccgtacc agttctgcca
      781 ggttctccgg aatcgtgggg tcagggtgat gattgggaaa acgcccatcg accgtgcagt
      841 acaattcctc gacctcacaa cccagatccc gcatcagttg aggaaacacg aggccggcgg
      901 tgccgttgcc cccatcgatg acgaccttca cgggacgaat gctttccttc aagaccgcat
      961 gtcgtttctg ctggtgttct cggtaagcct gaagaatgtc gtacgaatcg acagtcccgg
     1021 ttccataggc atactcatct ttttcgatca ggcgtctgag ctcctgaatg tctgcgccga
     1081 agatggtcgt cttgccaagg cagagtttga acccgttgaa ctcgctcgga ttgtggctgc
     1141 cggtaatctg aacggcgccg tcaacggcaa ggtggaagag agaaaagtag acgaccgggg
     1201 tggggcaggg tccgagttcg gtaacgttga caccggtttc cagaaggcct ttgacgatgg
     1261 catcatgcaa gctggggctc gaaaggcgaa catcctgacc cagggcgaga tgaggctgag
     1321 actttcccgt gtgtcgtctg acaaaagtgc cgaacgcgcg gccgatggcc tgcacaaaag
     1381 gtgccggcaa gtcccggtcg accacgccgc gaatatcgta ttccttgaaa acgtgacgat
     1441 tcggaatgtt catgtgggag ttccttgtac cgttggggga gtggatgaag aggagtcggc
     1501 gagcttcaat tgtctcaaat agagggcgaa tcgcagtgaa cgtacaagta tctgaatgcc
     1561 aaagaaaaga accagagccg ccatggccag gtgaagatat ccggccttga tgttcggcgg
     1621 tggcagccag tcgtcgacgg tcggttcacc ggcttccttt gctcccttca gatacatcag
     1681 acgcggcagc ttgaacgggt cctgatcgcg cttgtgttca agggcatcaa tcgtgaatgc
     1741 caggctgagc ccggccgaag cgacggcgag tttcgccgcg tcgctgtact cgccgaacgg
     1801 atacgcgtac cacgtgacct gtttgcctga gacctgctgc agcacctggc gcgcttgtac
     1861 gagttcgtcg gtcagattgc caccctcagc aagaatcttg gtgacctcat aatgacgctt
     1921 gccgtgaaga ccgataccga atccttctga tgccagagat cgaatctctt cggcgttcag
     1981 ataccctggc ttgccgatca tatcggtgat tacgaagata agcgatggta cgcccgcttc
     2041 ggtt
//
LOCUS       DAORHD010000982         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69703_length_2041_cov_2.630916, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000982 DAORHD010000000
VERSION     DAORHD010000982.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69703_length_2041_cov_2.630916"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..756)
                     /locus_tag="PKO06_17755"
     CDS             complement(235..756)
                     /locus_tag="PKO06_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71554.1"
                     /translation="MLTWMTCLGRFDLNRLWASRPTRESERFAFTLVEVLIAILLISI
                     LFSLGVLFTSSNSTTRKMRNFEYAIALAQQAVEVLRASPFDTLDSLDAQKKSLEEDFN
                     QDDPEPDLYRRTTRIGEIVYERTVTVEEVRNADEKKGINPRLKYVLVTVAWTPPDGER
                     LTYKLTTTIADLN"
     gene            970..1632
                     /locus_tag="PKO06_17760"
     CDS             970..1632
                     /locus_tag="PKO06_17760"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV71555.1"
                     /translation="MRRPILTLATLFVVISLVFLTGCGGGGSPAASQAIGRSPEAALR
                     ESLSGWQTGGGPLLPLVSSPVPAVSGSGSYGSITFRDLSNGTWTFDITNIIYFGDDLA
                     EIDTVFTTTYAATDTAKINFTMVRENGEWVIENIEVYLPPAGISTSGGTLQGVVTDAL
                     YGSSSPTPVSGALVYISGTSLQAVTDSNGYYEINGIPPGTYQIVITRDGYAPQTFTRT
                     IS"
     gene            1672..>2041
                     /locus_tag="PKO06_17765"
     CDS             1672..>2041
                     /locus_tag="PKO06_17765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71556.1"
                     /translation="MNTRTNATWNVLGLFLGIAVTVSLILGCGGGGGGGGNPVAPANQ
                     QLADLRGTVTFNGAPVADAAVYLVKPENALNAGMNNAGATGNIIASPTVSPLIRANTA
                     FGDYDTTTNQNGEYTFSQVPV"
BASE COUNT          470 a          569 c          556 g          446 t
ORIGIN      
        1 gcaccgactc gatagactgt tgaaccatgc gggtctgcga gaaaaacacc cggtacgtgg
       61 caaacagaat aatggtaaac agcaaaacca cgaccagtaa ttcaggcatg gtcaaagcac
      121 ttgtgttgag agatttggcg ctgaacaacc cggacagccg cctcatggat cgcaaggtca
      181 tgccacgcgg ccaactcgaa ggaagggttt tcgataaatg cttcatgagt tgtttcagtt
      241 caggtcagca atcgtggtag tcaacttgta ggtcaggcgc tcgccatccg ggggcgtcca
      301 ggcgacggtg acgagtacat atttcagacg agggttgatt ccctttttct catcggcatt
      361 gcgcacttcc tcgacggtaa ccgtgcgctc atagacgatc tcaccaatgc gggtggttcg
      421 tcggtagaga tccggctccg ggtcgtcctg gttgaagtct tcttccaggc ttttcttctg
      481 ggcatcgagt gaatcaaggg tatcaaacgg cgacgcccgc aatacttcaa ctgcctgttg
      541 cgccagagcg atggcatact cgaaatttcg cattttgcga gtcgtggagt tgctcgacgt
      601 gaacaaaaca cccagtgaaa acagaatcga gatcagaaga atggcaatca agacctccac
      661 cagggtgaag gcaaaccgct cagattcacg ggttggtcgt gatgcccaca atcgattcag
      721 atcgaacctc ccaagacatg tcatccatgt cagcatacca taacccgaat ctcctagaca
      781 caagacggtg gaatgttccg tatgtaacga ttgatgaaca aaaatggaat gtgcagtata
      841 cgctgaacac gaacaattgt gagttttctg cgctgattct gttctgtttc ggatgcgggt
      901 tgtgctatac tgctaccgtt ttcattccgg tcaccagaca agtggttccg gaatcgagag
      961 gaggaccctg tgcgacgccc tatcctgacc ctcgcaacac tgttcgtcgt catttcgctc
     1021 gtgtttctga ccgggtgcgg cggaggaggc agtcctgcgg cgtcacaagc catcggtcgg
     1081 tctccagaag cggctctgcg ggagagtctt tccggctggc aaacgggcgg tgggcctcta
     1141 ttaccgctgg tatcctcgcc ggtaccggct gtcagtggtt cgggcagcta cggcagcatc
     1201 acctttcgcg acctttcgaa tggtacctgg accttcgaca tcaccaacat catctacttc
     1261 ggtgacgacc tggccgagat cgacaccgtg tttaccacga cctatgctgc gaccgacacg
     1321 gcgaagatca actttacgat ggtacgagag aacggcgagt gggtcatcga aaacatcgaa
     1381 gtgtatctgc caccggccgg catcagcacg agcggtggaa cactgcaggg cgtggtcaca
     1441 gatgccctgt atggttcgag cagccccacg ccagtttctg gagctctggt ctacatttcg
     1501 ggtacctcct tgcaggccgt gaccgacagc aacgggtact acgagatcaa cggcatcccg
     1561 cccggcacgt atcagatcgt gattacccgt gacggatatg ccccgcagac ctttaccaga
     1621 acaatcagtt aaccatttct tgatttgtac aagcacacag ggagcgaagc aatgaataca
     1681 cgcaccaatg ccacatggaa cgtcctcggt ttgtttttgg gaatcgcggt taccgtcagc
     1741 ctgattctcg ggtgcggcgg tggtggcggg ggcggtggca atccggttgc ccccgcgaat
     1801 cagcaactcg ctgatctccg cgggacggtc accttcaacg gggctcccgt cgccgatgcc
     1861 gccgtctatc tggtcaagcc ggaaaacgct ctcaatgccg gcatgaacaa cgccggggct
     1921 acgggcaaca tcatcgcctc tccgaccgtt tcgccgctca tcagagcaaa caccgcgttt
     1981 ggtgattacg acaccacgac gaaccagaat ggcgaataca ccttcagtca ggttcccgtc
     2041 g
//
LOCUS       DAORHD010000983         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69704_length_2041_cov_2.624874, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000983 DAORHD010000000
VERSION     DAORHD010000983.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69704_length_2041_cov_2.624874"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKO06_17770"
     CDS             <1..297
                     /locus_tag="PKO06_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71557.1"
                     /translation="YLQITFSRVVLPDGNASDSTNYSLTGTNTLNLAFNWAGMQVASD
                     GKMFTLKLVPGAVNAFVPGSDTLSIDPAGPGLYDLAQPSANRCLTEQKVIVQPQ"
     gene            328..1035
                     /locus_tag="PKO06_17775"
     CDS             328..1035
                     /locus_tag="PKO06_17775"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71558.1"
                     /translation="MRRGFTLIELLVVITILAVLAGAALPYVQSYVEESKVAKAKTDL
                     EEIARALAVYETREGEYVKNTVEDLTGRYLNKAPIDPWGKSYVVATDSGTVYSSGPDR
                     TASTKEDNVTVPYQPPLALVQVKWVDKNQTGAVDIKNTPDQVQLIFSRRIQNTVANIS
                     TNFNTYFGTSSGSANFMFDDSTITSLASKTLVIDVLADNRFTPGSDTIKIANTHALTD
                     MAGSLALSDQSVIIVPQ"
     gene            1062..1778
                     /locus_tag="PKO06_17780"
     CDS             1062..1778
                     /locus_tag="PKO06_17780"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71559.1"
                     /translation="MLRKGFTLIELLVVITILAVLAGAALPYVQSYVEESKIAKAKTD
                     LEEIARALAVYETREGEYLKATVEDLTGRYLNKAPIDPWGKSYVVATDSGTVFSSGPD
                     RTASTKEDNVVVPYQPPLALVQVKWVDKNQTGAVDIKNTPDQVQLIFSRRIQNSISDI
                     ATNFTTYFSTSSGAANLMLDPTAGNMVSLASKTIVMNVVADNRFTPGSDTLNIATTHN
                     LKDMAGNAALPDQKVRILPQ"
     gene            1839..>2041
                     /locus_tag="PKO06_17785"
     CDS             1839..>2041
                     /locus_tag="PKO06_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020161723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71560.1"
                     /translation="MLGRKGVTLIELLIVVTILSILAGAAIPYVQDYVEDSRIGRARA
                     DIDEIRNALARYEVERGQAYTINT"
BASE COUNT          526 a          490 c          511 g          514 t
ORIGIN      
        1 tatctccaga taacattttc acgagttgtt ctccctgatg gaaatgcgag tgattcgaca
       61 aactattcct tgactggtac gaatacattg aatcttgcct tcaactgggc tggaatgcag
      121 gtcgcctcag atggaaaaat gttcacgctg aaacttgttc ccggagcggt gaatgctttt
      181 gttcctggca gtgacacgct ttcgatcgat cccgcaggac caggtcttta cgatcttgct
      241 cagccaagtg ccaatcggtg tttaactgaa cagaaagtca ttgttcaacc acaataggaa
      301 agagtcttcg gaaaggagaa tggtgttatg agacggggct ttacattgat cgagctgctg
      361 gtggtaatca caattctagc tgttcttgct ggcgcagcgc ttccttacgt gcaatcgtat
      421 gtggaagaat ctaaagttgc gaaagcgaaa acggatctgg aggagatcgc tcgcgcattg
      481 gcagtatacg aaacccgcga gggtgaatat gtgaaaaata ctgtcgaaga tttgactggt
      541 cgttatctca acaaagctcc aatcgatcct tggggcaagt cttacgtcgt tgcgacagac
      601 tctggtactg tgtattcttc aggccctgac cggactgcct cgacgaagga agacaacgtg
      661 actgttcctt atcagccgcc tctggcactc gtccaggtaa aatgggttga caagaatcag
      721 accggtgcgg tcgacatcaa gaacacccct gaccaggttc aattgatctt cagccgtcga
      781 attcaaaaca ctgtggccaa tatatcaacc aacttcaaca cctattttgg cacatcgagt
      841 ggttctgcaa acttcatgtt tgacgattcc acgatcacgt ctctcgcttc gaagaccttg
      901 gtcattgatg ttcttgcgga caacaggttc acacccggtt ccgacacgat taagatagcc
      961 aatacgcatg ctctcacaga tatggctggg agccttgcgc tgtccgatca gagcgtcatc
     1021 atcgttccac aataagatta ggcatacatg gaggtggcat catgttaaga aaaggcttta
     1081 ccctcatcga actattggtc gttattacca tcctggcagt tcttgctggg gctgcgcttc
     1141 cctatgtcca gtcatatgtt gaagagtcga agatcgccaa agcaaagacg gacttagagg
     1201 aaatcgcccg agctctggct gtctatgaga cgcgagaagg agaatacctg aaagcgaccg
     1261 ttgaagattt gactggccga tatttaaaca aggctccgat tgacccgtgg ggaaagtctt
     1321 atgttgtcgc aactgattct ggaacggtgt tttcgtcggg acctgacaga actgcttcta
     1381 caaaagaaga caacgttgtc gtaccgtatc agccgccctt ggcgctcgtg caggtaaaat
     1441 gggtcgacaa gaaccagact ggagcggttg acatcaagaa tacgccagac caggttcagt
     1501 tgatttttag ccgccgaata cagaattcga tcagtgatat tgcgacgaac ttcacgacgt
     1561 acttttcaac atcgagtggt gccgcgaacc ttatgctgga tcccactgca gggaacatgg
     1621 tttcgcttgc ttcaaaaact atcgtcatga atgtggtcgc ggacaatcgt tttaccccgg
     1681 ggtctgacac gctcaatatc gctacaacac ataacctcaa agatatggcc gggaatgcgg
     1741 cccttcccga ccagaaagtc cggattctgc cgcagtaagt ggtacggttt ctgctagttt
     1801 gtttcattgc tggatacaaa aacatggagg gcagtcatat gttagggaga aaaggtgtta
     1861 cgctgatcga gttgctgatt gtcgtcacga ttctttcaat tctcgccggt gcagccattc
     1921 cgtatgtgca ggactacgtc gaagactccc gtatcggccg agccagggca gacattgatg
     1981 agatccgaaa cgccctcgcc cgctacgaag tcgagcgtgg tcaggcgtat actatcaaca
     2041 c
//
LOCUS       DAORHD010000984         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_69848_length_2038_cov_3.788200, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000984 DAORHD010000000
VERSION     DAORHD010000984.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69848_length_2038_cov_3.788200"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKO06_17790"
     CDS             <1..820
                     /locus_tag="PKO06_17790"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71561.1"
                     /translation="LLEGEVQFGVILPGTASSSEQASGTPTLTLIHPTGLSAFMVERL
                     KGDFEREIRKIFHLPEPRLPIAVELSPVGGSKDGERSFSSDFFPMLILMAMGMVGFLG
                     MPISLVEEKERRTLHALFLTPIRPNELIIGKSLFGLALILFTVVTMVFINNKWLGDQF
                     YFWLIVLMSSLFCLFLGLLISLLASSQASVNALGTSLFMIFQLVPNLSQTSEVLRRFA
                     WLVPSTYVSRALKKALFLDLSKVEIWQDLAVIGGCAMVTYTVVFCMLRWRQYKI"
     gene            complement(817..>2038)
                     /locus_tag="PKO06_17795"
     CDS             complement(817..>2038)
                     /locus_tag="PKO06_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71562.1"
                     /translation="LADDPRQHVKRRRGYYDGILLLPADPVSLSANRLFTREAFREYA
                     SALRSNGLFSFSLTGTENYLGDEMEQTLLSAHRTARAIFPGVFAIPGDPVTFWCSPNA
                     AALATDPERLATRFADRSIVTSSFSTHSFRNLLLPFRVDELHRWLQRSIPVSENSDLK
                     PTAFTQQLRLWNVYTDSGIGPLLTRALALTTGHFALGLTGLLLLLLIGQRLLAATGRE
                     TLTIHACAGISGSVGILLELALIFLYQVRCGALFHLLALFFGLYMCGLALGAGLAGRC
                     RALTGWELPVTKIGQVLAVFLGGLAVLSPAEFTGWCTGSAIFLFALLAGFEFAILDRL
                     LRERGTQAGRSAGALLLSDNGAAVCAAVLSGPILLPTAGIFGTLQIALVALLGNLLLV
                     LFGLSPTNQQTSKA"
BASE COUNT          431 a          560 c          652 g          395 t
ORIGIN      
        1 gctgctcgaa ggcgaggtgc agttcggcgt catccttccc ggtaccgcct cgtcgagcga
       61 acaggcaagc ggtacgccca cgttgaccct gattcatccg accggccttt ccgcgttcat
      121 ggtcgaacga ctgaaggggg atttcgagcg cgagatccgc aagatctttc atctgcctga
      181 accgcggtta cccatcgcag tcgagctgtc acccgtcggg ggctcgaagg acggggaacg
      241 gtcgttctcg agcgactttt ttccgatgct cattctgatg gccatgggaa tggtcggatt
      301 tctcggcatg ccgatctcgc tggtcgaaga gaaagagcgg cgcacgctgc atgccttgtt
      361 tctgaccccc attcgcccga acgagctgat catcggcaag agtctgtttg gtctggcctt
      421 gattctgttt actgtcgtca ccatggtctt catcaacaac aagtggcttg gcgatcagtt
      481 ctatttctgg ctgatcgtct tgatgagctc gctgttctgt ctgtttctcg ggttgctgat
      541 atcgctgctg gcttcaagtc aggcatcggt caacgctctg ggaacctcac tgttcatgat
      601 ctttcaactg gtacccaacc tgagtcagac cagcgaagtg ctgcggcggt ttgcctggct
      661 ggtgccgtcg acgtatgtgt cgcgtgcgct caagaaagcg ttgtttctcg atctgagcaa
      721 ggtcgagatc tggcaggacc tcgcggttat cgggggctgc gcgatggtga cctacaccgt
      781 ggttttctgc atgctgcgct ggcggcaata caagatttaa gctttcgaag tctgctggtt
      841 cgtgggtgaa agcccaaaga gtacgagcag tagattgccg agaagagcga ctagagctat
      901 ctgtaacgtg ccgaaaatac cggcggtggg cagaagaatc ggaccgctca acacggcggc
      961 acagacggcc gcgccattgt cgctgagcag cagcgctccg gccgagcgac cggcctgcgt
     1021 gccacgctca cgcagcaggc gatcgagaat agcgaactca aaaccggcga gcagagcgaa
     1081 cagaaagatt gcgctgccgg tacaccagcc ggtgaattcc gccggactga gcacggccag
     1141 gccgcccaga aagacggcca gaacctgacc gatcttcgtc acggggagtt cccagccggt
     1201 cagggcgcgg catcgaccgg ccagaccggc accaagtgcc agtccgcaca tgtagagtcc
     1261 aaagaacagg gccagcaggt gaaacagagc gccgcaccgc acctgataca gaaaaatcag
     1321 agccagttcg agcaggattc cgaccgaacc tgagatgcct gcgcaggcgt gaatggtcag
     1381 ggtttcccgc ccggtggcgg cgagcaggcg ttggccaatc agcagaagca gcagcagtcc
     1441 ggtcagtccg agggcgaagt gaccggtcgt gagagcgaga gcgcgggtaa gcagggggcc
     1501 gatgccagaa tcggtgtaga cgttccagag gcgcaactgt tgcgtgaaag cggtcggttt
     1561 cagatcggag ttttctgaga cgggaatcga gcgttgcagc caccgatgca gttcgtcaac
     1621 cctgaacgga agaaggagat ttcgaaagct gtgcgtcgaa aacgagctcg tgacaatgct
     1681 ccggtcggcg aaacgtgtgg ccagcctttc agggtcagtc gccagcgcgg cggcattcgg
     1741 cgaacaccag aaggtgaccg ggtcaccggg aatggcgaag acgcccggaa aaatcgcgcg
     1801 tgcggttcgg tgtgccgaga gcagggtttg ttccatctcg tctccgaggt agttctcggt
     1861 accggtcagc gagaacgaga agaggccatt tgagcgcaaa gcagaggcgt attctcgaaa
     1921 agcctcgcgg gtgaacaggc ggttggccga taggctcacc ggatcggcgg gcaacagcag
     1981 gatgccgtcg tagtagcccc tgcggcgttt cacatgctga cgcgggtcgt ccgcaaga
//
LOCUS       DAORHD010000985         2035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70011_length_2035_cov_4.288384, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000985 DAORHD010000000
VERSION     DAORHD010000985.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70011_length_2035_cov_4.288384"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKO06_17800"
     CDS             complement(<1..183)
                     /locus_tag="PKO06_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71563.1"
                     /translation="MALALIAVLIVVSLNAQEETHSHREEDHSMLRRKTVALIIAPEN
                     FRDEELFVPKQEFEQAG"
     gene            complement(227..1873)
                     /locus_tag="PKO06_17805"
     CDS             complement(227..1873)
                     /locus_tag="PKO06_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71564.1"
                     /translation="MGAKPSLDQLKQVLFQPNPALKKRALAIIFNEAMCEARPLLEEY
                     VGQENNPELQALALKVLNKLETPQQKPQALSPEQLMAALPSATPAKKIELLRALSQCR
                     SPRLAVTIQQLFSQENDPDALLAIAEIFRNNPHIGNLGILMRLSAHQNEKLRLAALES
                     ILNVMFGCMYPFILKALLDPSVPLKMKAYQLISTISRANLLEALGYMLENENPQFSRL
                     AGQLLPSFMGPDLIELLKKHLKHPDQETATHCLRTVTLLAQKGFGEAIVLLETLGETR
                     AEAAHRRAAPTIPASLQTMLNGLPSWFSEPLARKPDDIRQLLNGIREVYERCRDFLIL
                     SFVCTYITMGKRNMTLDQTVFKVMTQGVSRTDPVALLPALAISFPEAREQGDIFPLVL
                     ANRIVYEPDDTLVEDLISLQEGLKLVTDHPEELAKVIEPALQGLEKFLFSLSSMKANR
                     LLVKFNDTEGMKVGDFFQRELATVDPRTVTHLELPLNRPLVVSQTGAYGLAVWPFVVF
                     NPYQKKLLRLDPTEQDLWELLVQHNMLDGFMTHLKQKPAS"
BASE COUNT          419 a          501 c          647 g          468 t
ORIGIN      
        1 tccggcttgc tcgaattcct gcttgggaac gaatagttcc tcatcgcgga aattctctgg
       61 tgcgatgatc agtgcgacgg ttttgcggcg caacatggaa tgatcctcct cacgatgact
      121 atgtgtctct tcttgtgcgt tcagcgaaac aacgatcagg acagcgatca gggccagggc
      181 catgcgaaca gtcgaacgga atggcatgaa gacgggaaag gacgggtcag gacgcgggct
      241 tctgcttcaa atgggtcatg aagccgtcaa gcatattgtg ttgtaccagg agttcccaga
      301 ggtcttgctc cgtggggtcg agtcgcagga gttttttctg atacggattg aagaccacga
      361 acggccagac cgcgagcccg taggcacccg tttggctgac gacgagcggc cggttgagcg
      421 gaagctcgag atgagtgacg gttcgtgggt cgaccgtggc aagctcacgt tgaaagaagt
      481 cgcctacttt cattccctcg gtgtcgttga acttcaccag caggcggttt gctttcatcg
      541 atgagagtga gaacagaaac ttctcgagcc cctgaagcgc cggttcgatc accttggcca
      601 gttcttcggg atgatcggtg acgagcttca agccttcctg aagtgagatg aggtcttcga
      661 ccagcgtgtc gtcgggttca tagacgatgc ggttggcgag aaccagtggg aagatgtcac
      721 cctgttcacg tgcttccggg aacgagatcg ccagggcagg taacaatgca accgggtcgg
      781 ttctcgacac gccttgtgtc atgactttga acacggtctg gtcgagcgtc atgttgcgct
      841 tgcccatagt gatgtaggta caaacgaacg acaggatcag gaagtcgcgg cagcgctcgt
      901 acacttcgcg aatgccattc agcagctggc gtatgtcgtc gggtttgcgg gcgaggggtt
      961 cggaaaacca gctgggaagg ccattgagca tcgtctgcag actcgcggga atcgtggggg
     1021 cagccctgcg gtgtgcggct tcggctctgg tttctccgag ggtttcgagc aggacgatcg
     1081 cttcgccgaa ccctttctga gccagcaagg taaccgttcg cagacagtgc gtcgcggttt
     1141 cctgatcggg gtgtttcaga tgcttcttga ggagctcgat gagatccggg cccatgaatg
     1201 aggggagcag ttgtccggcc agccgcgaaa actgcggatt ctcattttcg agcatatatc
     1261 caagtgcttc aagaagattg gcgcggctga tcgtgctgat caactggtac gctttcattt
     1321 tgaggggcac ggaaggatcg agcagggcct tgagaatgaa cgggtacatg cacccgaaca
     1381 tgacgttcag tatgctctcg agtgccgcga ggcgcagctt ctcgttctgg tgcgccgaga
     1441 ggcgcatcag aatgccaagg ttgccgatat gggggttgtt gcggaagatc tcagcgatgg
     1501 cgagcagcgc atccggatcg ttttcttgag agaagagctg ttgaatcgtg accgcgaggc
     1561 gaggcgatcg gcattgcgac agggcgcgca gcagttcgat cttctttgcg ggagtggcgg
     1621 agggaagagc agccatcagt tgctcgggag acaacgcctg cggtttctgc tgcggggttt
     1681 cgagcttgtt gaggaccttg agggcgagcg cctggagctc gggattgttt tcctggccga
     1741 cgtattcttc gagtaacggc cgggcttcac acatggcctc attgaaaatg atggccaacg
     1801 cacgcttttt cagtgccggg tttggctgaa acagaacctg tttaagttga tccagagatg
     1861 gtttagcgcc catgtttcct cccactcgca gaagagtcta cccgaaaagt gtcgttcgtg
     1921 caacggtcgt tctgaggctg aacatttgac gggatcatga gtctatgcta aatttcgccc
     1981 gtcgcagcgg ataccgcctg tgtttccttc cgaacgggcg accagatccg aagag
//
LOCUS       DAORHD010000986         2033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70172_length_2033_cov_2.136502, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000986 DAORHD010000000
VERSION     DAORHD010000986.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70172_length_2033_cov_2.136502"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..670
                     /locus_tag="PKO06_17810"
     CDS             95..670
                     /locus_tag="PKO06_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HNV71565.1"
                     /translation="MKRPTLQFYFDFLCPYSYIALELMRRTLDQKQLEVEYICPGLRT
                     PGVDVDERSFWQPDRWGRIAKAAEKLGLTIRPPAPTANSVLVRRGLRKYAGIGLAEYI
                     GSVFKGVFTAGVDISKERLLIEFLQSDGADIKPLEEALNDSGTALAAAEDSNLWVTQR
                     LRLLPTFSLSNERYAGMIDARGLENFLLRYL"
     gene            680..1333
                     /locus_tag="PKO06_17815"
     CDS             680..1333
                     /locus_tag="PKO06_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV71566.1"
                     /translation="MSFTYEYPRPMGTADAALVSPERERPEILLIRRGQAPYAGCFAL
                     PGGFMDLDETPQRTAERELQEETNLSGIALRPLFTCAQPGRDPRGRSLTMVYGALVQA
                     SSLTPRGGDDAAEARWFCLDELPEMAFDHARVVAQIAAHLRWQASTSVIGRDLLPADF
                     SAEALLALHERVLGRRCTESPLDRGIRLGLIQRAANGCWRFVPPADSGYPDWEPLVW"
     gene            1356..>2033
                     /locus_tag="PKO06_17820"
     CDS             1356..>2033
                     /locus_tag="PKO06_17820"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71567.1"
                     /translation="MNLLRPTIIAFSRGFTKRDPKLLITLVIAALIIAVGIYFGLYKF
                     LSHVGRAPMLGDYLGPLVGSLLVSKLLEMIFLALFFMIVFSSIIAAFSVFYLDEEIRL
                     LMATPVRISSIFWSRFALMLVESSWMALVFFIPIFYAFITAADAPITVYFIFPVLIGL
                     YLLAPNVVGALLALILGRLFPVRQMRKVFQFLSVLVLASMVFFFRYLEPEKLLNPNHF
                     GSVSAYIL"
BASE COUNT          365 a          559 c          582 g          527 t
ORIGIN      
        1 cgggactgag cgaatgagaa tgaagaaccg atgggaatgg attgtgtcgg catgcttccc
       61 ctgatataat cactgtgcgg aaaagaggtg ccctgtgaaa cgtccaacgc tgcagtttta
      121 ctttgatttt ctctgcccct attcctatat tgcgcttgag ctgatgcgga gaacactcga
      181 ccagaagcaa ctggaagtcg agtatatttg tccgggtctg cgaactcccg gtgtcgatgt
      241 tgatgaacgc tcgttctggc agcctgaccg gtggggtcgc atcgccaaag ccgcagaaaa
      301 actcgggctg accattcgtc cgccggctcc cacagccaat tctgtgcttg tgcgccgggg
      361 gctgcgcaaa tatgccggaa tcggactggc ggaatacatc ggcagtgtgt tcaagggcgt
      421 gttcacggct ggagttgata tcagcaaaga acgtttgttg atcgagtttc tgcagagcga
      481 tggagctgac atcaagccgc tggaggaagc gctcaatgat tctggcaccg ctctggcagc
      541 agctgaagac agcaatctct gggtcacgca acggttgcga ctgttgccga cgttttctct
      601 cagcaacgag cgttatgcag gcatgattga tgcccggggc ctcgaaaact ttcttctgcg
      661 ctatctctga gctgtcgtca tgagtttcac ctatgagtat cccagaccga tgggtacggc
      721 cgacgcggct cttgtctctc ccgagcggga acgacctgag atcctgttga tccgtcgtgg
      781 acaagccccc tatgccggtt gctttgcctt gcccggaggc ttcatggatc tggacgagac
      841 accacagcga accgcggaac gcgaactgca ggaagaaacc aatctctcag gaatagcgtt
      901 gcgaccgctg ttcacctgcg cgcaaccagg acgcgacccg cgcggtcgta gtctgaccat
      961 ggtgtatggt gctctggttc aagcctcgtc tttgactccc cggggagggg acgatgccgc
     1021 ggaagctcgc tggttctgcc tcgatgaact tcccgagatg gccttcgatc atgcccgcgt
     1081 cgtggcccag atcgccgcac atctgcgctg gcaggcgtcg acgtcggtta tcggccgtga
     1141 tctgctgccg gccgactttt ctgccgaagc attgctggct ctgcatgaaa gagtgctcgg
     1201 ccgtcggtgc actgagtccc ccctggatcg cgggattcgc cttggtctga tccagcgagc
     1261 tgcaaacggg tgctggcgtt ttgtcccacc tgctgactcc ggatatcctg attgggagcc
     1321 tctggtgtgg tgagtctccc cgtactggca ccgttgtgaa tctgcttcgt ccgaccatca
     1381 tcgccttctc gcgtgggttt accaaacgcg atccgaaact cctgatcaca ctggtgatcg
     1441 cggctctgat tatcgccgtt ggaatctact tcggcttgta caaattcctc agtcatgtcg
     1501 gtcgcgcgcc gatgctggga gactacctcg gaccgttggt cggaagtctg ctggtctcga
     1561 aacttttaga gatgattttt ctggcactct tcttcatgat cgtcttcagc agcatcattg
     1621 ccgcgttttc cgtgttttac ctcgacgagg agattcgatt gctcatggca acgccggtgc
     1681 gcatctcgag catcttctgg tcgaggtttg cgctgatgct ggtcgagagc tcgtggatgg
     1741 cgctggtgtt ctttatcccg attttttatg cgttcattac cgcggctgat gccccgatta
     1801 cggtgtattt catctttccc gtgttgatcg gactgtatct cctggccccc aatgtcgtgg
     1861 gagcattact ggcgctgatt ctggggcggt tgtttccggt gcgtcagatg cgcaaggtgt
     1921 ttcagtttct ttcggtactg gtgctggctt cgatggtgtt cttctttcgc tatctcgagc
     1981 ctgaaaagct gctcaatccc aaccatttcg gcagcgtatc cgcgtacatt ctc
//
LOCUS       DAORHD010000987         2031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70263_length_2031_cov_3.224696, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000987 DAORHD010000000
VERSION     DAORHD010000987.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70263_length_2031_cov_3.224696"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..1346
                     /locus_tag="PKO06_17825"
     CDS             318..1346
                     /locus_tag="PKO06_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71568.1"
                     /translation="MNRQLRSMIVLWCGVIWLFAYGSSFGAGMETAATSASGLEFFPL
                     EKGNMWVYEGPVTWTPAGGKSTTATLTVTMEVLETIERDHVFAALVSGYPGDTAMYDP
                     GEAREPSLIVRVGPVWYYLISGRDRISSITKRLRDQDDVLFDLVQPGDLWLMSPLIPG
                     CLYGEADQITRTDRSYCWQVEAVEEANLETYLPQTKTSGRKYRLANRVSSSYEQLDFV
                     PGVGVVEYAYVHHGTPFESHLRLLRCRVRSRDRETGAPSSSAVTPPVSEPVSLERALQ
                     LALEHVKQKGVDLTAQHLGSVTRQYDAAKKQPYWHVQWQWNTPKMGGEYGLAVFMDGT
                     ISDRRCGP"
     gene            1397..>2031
                     /locus_tag="PKO06_17830"
     CDS             1397..>2031
                     /locus_tag="PKO06_17830"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNV71569.1"
                     /translation="MATLGQINSLKVVKSLDFGVYLDGGELGEILLPRRQVPDECQIE
                     DTLDVFLYHDTENRLIATTHRPVAVLDQVGFMQVKSVNDVGAFLDWGIPKDLFVPFRE
                     QSHPMRVGQSYLVYVYFDKVSERITGSTKLRKFLNKPWFSVTNGQEVDLIIAEKTDAG
                     YKAIINHAAWGLIFNSDVFQSLTVGQSVKGFVKAVRDDGKYDLVLQKPGFE"
BASE COUNT          434 a          521 c          604 g          472 t
ORIGIN      
        1 agataccgaa gctggacctc gttgtccgcg ccgcgttccc ctgccccgtg cagagaaagc
       61 aacacgggcc agccgtcggc aggggtcggc cgccggggaa cccacgtctg caccggaaac
      121 atctccccgt cgacttcagt gatgctgcga atccagacgc tttccatgtt ccgtcctcca
      181 tgaggttcag gtgctttcag ccgggtggtt cattggttcg ggtatcctac atcaccggct
      241 ccgcaaacgc gatggtttgt caggccggga tcggcatgat atgctcgcat cagaacagga
      301 aagcgggagg aggattcatg aaccggcagt tgcgatcaat gattgtcttg tggtgcgggg
      361 tgatctggtt gttcgcgtat ggctcgtcat tcggggcagg aatggaaaca gcggcaacgt
      421 ctgcatcagg tcttgaattc tttcctcttg agaagggcaa tatgtgggtg tatgaaggtc
      481 cggtaacctg gacccccgca ggcggcaaat ccacgacggc aacgctgacg gtgaccatgg
      541 aagtgcttga aaccatcgaa cgcgatcatg tgtttgccgc gctggtcagt ggatatccag
      601 gcgatacggc aatgtatgac cccggtgagg cgcgcgaacc gtcgcttatc gttcgagtgg
      661 ggccggtctg gtactatctg atcagcggtc gtgaccggat cagttcaatt accaagcgcc
      721 tgcgtgatca ggacgacgtg ctctttgatc ttgtccagcc tggtgatctg tggttgatgt
      781 cgcccctgat acccggttgt ctctatgggg aagccgacca gatcactcgc accgatcggt
      841 cttattgctg gcaggtcgag gccgttgagg aggcgaatct cgagacgtat ctccctcaga
      901 cgaaaacttc agggcgaaaa tacaggctgg ccaatcgcgt cagcagttca tatgaacagc
      961 ttgattttgt tccgggagtt ggcgtcgtcg agtatgcgta tgttcaccac ggaacccctt
     1021 tcgagagtca tctgcgcttg ctgcgctgtc gcgtgcgttc tcgtgaccga gagaccggtg
     1081 ctccgtcgag ttccgcggtc accccgccag tgtccgagcc ggtttctctg gagcgtgcac
     1141 tgcaactggc actggaacat gtgaagcaga agggtgtcga tcttacggca cagcatcttg
     1201 gctcggtaac ccggcagtat gatgccgcaa agaaacagcc gtattggcac gttcagtggc
     1261 agtggaatac ccccaaaatg ggcggtgaat atgggctggc tgttttcatg gatggtacaa
     1321 tcagcgatcg tcggtgtggg ccgtagagtc actgagcaat tccatcgaga taaggtcatt
     1381 ttcgcaggag aaatatatgg cgacactcgg acagatcaat tcgctgaagg tagtcaagag
     1441 tctggatttc ggagtatatc tcgacggcgg cgaactgggc gaaatcctcc tgcctcgacg
     1501 acaggttccc gacgagtgtc agatcgagga cacccttgac gttttcctgt atcacgatac
     1561 cgagaaccga ctgatcgcca ccacgcatcg acccgttgcc gtgcttgacc aggtgggttt
     1621 catgcaagtg aagtcggtga acgacgtcgg ggcctttctg gattggggaa tcccgaagga
     1681 ccttttcgtt ccctttcgag agcagagtca cccgatgcgg gtcggacaat cgtatctggt
     1741 ttatgtgtac ttcgacaagg tcagtgagcg tatcaccggt tcgacaaagt tgcgaaagtt
     1801 tctcaacaaa ccctggtttt cggtaacgaa cggtcaggaa gtcgatctga tcattgcgga
     1861 gaaaactgac gccgggtaca aggccattat caaccatgcc gcctggggcc tgatcttcaa
     1921 cagtgatgtg tttcagtcgc ttacagtggg tcagtctgtc aaaggatttg tcaaagccgt
     1981 tcgtgatgac ggcaagtatg atctcgtgtt gcagaagcca ggcttcgaaa a
//
LOCUS       DAORHD010000988         2029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70356_length_2029_cov_2.917933, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000988 DAORHD010000000
VERSION     DAORHD010000988.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70356_length_2029_cov_2.917933"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..1159)
                     /locus_tag="PKO06_17835"
     CDS             complement(119..1159)
                     /locus_tag="PKO06_17835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV71570.1"
                     /translation="MRYHFTALSLAILFVLAPLALAAQTSFPGIPGLCYHQVQPASGG
                     KYSLSKEGFRGQLLLIRQLGYTPINSERLLEILRSPEPPREKHVVITFDDGYRSVFDH
                     AFPIMREFGFVGVVCVYPEFIGTAGGMSWEQLRTLQKAGWSVDCHSSSHKDLGKPPTD
                     AAARAAFYEREIVRPKQIIEQKLGTPVLFMAWPYGIYTEETLAMAKKAGYAGAYTVDG
                     GGNYPGLDPFQVKRQVVYDTDTKDKFTIRLEMGSLKVEQHQPKPGAVLAGLNHVSCTL
                     PELADYTPDQYVLTAKITGGSLTPSFDTQTRTLRATTNSAPGKGTRFIDIYLRDKRTG
                     LTRQHGWFVIIK"
     gene            complement(1547..1759)
                     /gene="hfq"
                     /locus_tag="PKO06_17840"
     CDS             complement(1547..1759)
                     /gene="hfq"
                     /locus_tag="PKO06_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNV71571.1"
                     /translation="MPAINIQDTILNVLKAKAVPVKIFLMKGFQIQGIVLDFDTFVIL
                     LESEGKKQLIYKHAVSTIQLPDDFVS"
     gene            complement(1775..>2029)
                     /locus_tag="PKO06_17845"
     CDS             complement(1775..>2029)
                     /locus_tag="PKO06_17845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71572.1"
                     /translation="FGYFPCPVKAADRCAFSRQRLEMLGRQGSQQKNGDGLLKQLHID
                     YNIPPCPHGGVYVLDADGKVSCSLHSRSQPSQPVPAPQEK"
BASE COUNT          466 a          511 c          601 g          451 t
ORIGIN      
        1 gacccgttga tcccgtttcc gtctcggctt gcagacccca tgggagccga tgtggatccg
       61 cgcagcgacg aacgagcgcc gaagtaggtt cgccggctat agaggttgag ggggcgacct
      121 acttgatgat cacgaaccaa ccgtgttgtc gcgtcagacc ggtgcgcttg tcacggagat
      181 agatgtcgat gaatctggtt cccttgccag gcgctgaatt ggtcgtggct cgcagcgtgc
      241 gagtctgagt gtcgaaggaa ggtgtcaggc ttcccccggt gatcttcgcc gtgagcacat
      301 actggtccgg cgtgtaatca gcgagttcgg gaagagtgca gctgacgtgg ttcaatccgg
      361 cgagtactgc accgggcttc ggttgatgtt gctcgacctt cagcgaaccc atctcgagtc
      421 ggatcgtgaa tttgtccttg gtatccgtgt catagacgac ctgccgtttc acctgaaagg
      481 gatcaagccc gggatagttg ccgccaccat cgacggtata cgcgcccgca tatccggctt
      541 tcttcgccat ggcgagcgtt tcttcggtgt aaatcccata gggccaggcc atgaacaaca
      601 ctggtgttcc cagtttctgc tcgatgatct gctttgggcg tacaatctcg cgctcgtaaa
      661 aagcggctcg agcagccgca tctgtcggcg gctttccaag gtccttgtgc gagctggaat
      721 gacaatcgac gctccatccg gctttctgca gcgtgcgcag ctgttcccag gacatgcccc
      781 cggcggtgcc gatgaattct gggtagacgc agacgacccc cacgaatccg aactcgcgca
      841 tgatcggaaa ggcatgatca aacacggagc ggtagccgtc gtcaaaggtg atgaccacgt
      901 gtttctcccg gggtggttcg ggtgagcgca aaatctcgag cagacgttcg ctgttgatgg
      961 gggtgtaacc gagttgcctg atcagcagaa gctggccgcg aaacccttct ttggacaggc
     1021 tgtattttcc ccctgaggcc ggttgtacct gatggtagca gaggccggga attcccggga
     1081 atgaagtctg ggccgccagg gcaagaggag caagaacgaa cagaatggcc agtgacagtg
     1141 cggtgaaatg gtaacgcatg cggttcctcc gtgacagggt gaacaaatga aatgacctaa
     1201 aaaaaaagag actgcatgtg tccatgcagc caaatcaagg aaaggaatga ttgaaagcaa
     1261 ctgatagaga gatcgatcag ttgtgaactt ccatatgcaa atgccatgcc aggttgcgga
     1321 ggggcaaact gggcaaattg tgaacaaact gagggttttg gttcggaaaa ggctgcaaaa
     1381 aaagtgtaca ttacccggtt ccgtgtgtaa taactaggca ttggtggctg attgaggtgc
     1441 atacagtcga gcggagagta ccatgctttc agagaaagaa aaaaggcttt ccgtcaggaa
     1501 agcccgttga gtcacgatcg tgcaggaagc cggctggcct ccaggtttag gaaacgaaat
     1561 cgtcaggaag ctgaatcgtc gaaaccgcgt gcttgtaaat cagctgcttc ttcccctcag
     1621 attcgaggag aatgacgaag gtgtcgaaat cgagcacaat accctgaatc tggaacccct
     1681 tcatcaggaa gatcttgacg ggaactgctt tcgccttcag tacgttgaga atggtgtcct
     1741 ggatgttgat tgccggcata ttgtttcttc ctttctattt ttcttgcggt gctggcacgg
     1801 gctgagacgg ttggcttcgg ctatggagcg agcacgaaac cttgccgtct gcatcgagca
     1861 catacacccc cccatgcggg cacgggggta tattgtaatc gatatggagc tgttttagca
     1921 aaccgtcacc gttcttctgc tgagacccct gacggcccag catttcgagc cgctgccggg
     1981 aaaaggcaca gcgatcagcc gctttgaccg ggcagggaaa atacccgaa
//
LOCUS       DAORHD010000989         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70447_length_2027_cov_3.040568, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000989 DAORHD010000000
VERSION     DAORHD010000989.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70447_length_2027_cov_3.040568"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..379
                     /locus_tag="PKO06_17850"
     CDS             101..379
                     /locus_tag="PKO06_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71573.1"
                     /translation="MLERIDRLVFYFNVDCANCHNLMKVSHVTVEEGQGNIVCTLCGK
                     KVVVPNYEVLVKASKDLNLYLADSMNHKRIKLGLNELFKVEEAQLAAH"
     gene            complement(407..>2027)
                     /locus_tag="PKO06_17855"
     CDS             complement(407..>2027)
                     /locus_tag="PKO06_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71574.1"
                     /translation="LEKIERALQHDEDNDSLRTWYASITTHLEKIDKARQEAKARELN
                     RKQAELEAEKKNAVLLKAANEAERKRSFEAALINYEKLQAIDPKPAIAAKIEELKKTI
                     AEVKEKQKKLGNDLANATTLFSQEKYDEAYTVLKEVAEDPDAPAEELIVKLVQCCFRL
                     NRLEDAQKYTLKLESISSASTYLPYFNGSLAYRRGDYFAALDLLAPVYSQNPTIGTDL
                     GQMVWTARLFKYKWFFIVFGLMIGYSLLGPIRSWLADLKAAGLQHRINSAVGSANWAA
                     AIPLLQKRLEADLPLSERKAVMVQLADCCLKAGQPHEAKAQALEVLGKDSKHAQATRI
                     LGEALYQLRDNTPESIDRIMAAFRLDENRKDLLAFLVENFKSQQADSKTATDILLKHI
                     TVAHEDRDTLIYLAKMFFKRSEYTASSLKVFEKATKVAPDAPEYVHGLVQCLTATGRA
                     DDAAKWRDMGRQKWPDSSYFSGSVEQSFTAGAAIPGTAKIGLKRETPAPAAAEPKGKS
                     AGKGGEVLCKSCGAGNPAREYYCTSCGKPLK"
BASE COUNT          376 a          547 c          598 g          506 t
ORIGIN      
        1 cttgccggcg gtttttttcg tctgggtgac cttgacatcc cgagccgggg agaggacact
       61 gagatcgttt ttcaaacact ttttccggac aggagaagct atgctggaac gaatcgaccg
      121 cctcgttttc tacttcaatg tcgactgcgc caactgccac aacctgatga aggtcagtca
      181 cgtgactgtc gaagaaggac aggggaacat cgtctgcacg ttgtgtggca agaaagtcgt
      241 ggtgccgaac tatgaagttc tggtcaaggc gtcaaaagat ctcaacctgt atctggctga
      301 ttcgatgaat cacaagcgca tcaagctcgg tctgaacgaa ctgttcaagg tcgaagaagc
      361 gcagttggcg gctcactgag ccaacggatc ggggcggcgc gcgcgttcac ttgagcggct
      421 taccgcagct ggtacaatag tattcgcgcg ccgggttccc ggcgccgcag cttttgcaga
      481 gcacctcgcc acccttgccg gcagacttgc ccttcggttc ggccgcggcc ggggctgggg
      541 tttcgcgctt cagtccgatt ttggcagttc cgggtatggc ggctccggct gtaaacgatt
      601 gttcgacgga cccggagaag tagctgctgt cgggccattt ctgtcgtccc atgtcgcgcc
      661 acttggcggc atcgtccgct cttccggtgg ctgtgaggca ttgtacaagt ccgtggacgt
      721 attcgggggc gtcaggagcc actttggtgg ccttttcgaa gaccttcaga gaactcgcgg
      781 tatattctga ccgtttgaaa aacatcttcg ccagatagat cagcgtgtcc cggtcttcat
      841 gcgcgaccgt gatatgcttg agcaggatgt ccgtcgctgt cttgctgtca gcctgctgac
      901 tcttgaagtt ttcgacaaga aatgccagca gatctttgcg gttctcatcg agacggaatg
      961 ccgccatgat tcggtcgatg ctttccgggg tgttgtcgcg cagctgatac agcgcttcac
     1021 cgagaattcg tgtcgcctgc gcgtgcttgc tatccttgcc cagcacctcg agtgcctgcg
     1081 ccttggcttc gtggggttgc cccgctttca ggcagcagtc ggcaagctgc accatgacag
     1141 ctttacgttc cgacagtggc agatcggctt cgagtcgttt ctgcaacagg ggaatggccg
     1201 cggcccaatt ggcggaaccg acggcggagt tgatacggtg ctggagcccg gcggccttga
     1261 gatcggccaa ccatgagcga atggggccga gcaggctgta gccgatcatc aggccgaaga
     1321 cgatgaaaaa ccatttgtat ttgaacagac gagcggtcca gaccatctgg cccaaatcgg
     1381 taccgatggt cgggttctgg ctgtacacag gagcgagaag gtcgagcgcg gcaaagtagt
     1441 caccgcgtcg gtaggcgagt gagccattga aatagggcag atacgttgaa gcgctgctga
     1501 tggactcaag cttgagggtg tacttctggg catcctcgag tcggttcagt cgaaaacaac
     1561 actgaacgag cttcacaatc agttcttccg cgggggcatc cgggtcctcg gccacttcct
     1621 tgagaacggt gtacgcttcg tcgtatttct cttgcgaaaa aagagtcgtg gcattcgcca
     1681 gatcgttccc cagtttcttc tgcttctcct tgacttccgc tatggttttt ttcagttctt
     1741 cgatcttcgc ggcaatcgct ggtttggggt caatcgcctg aagcttttca tagttgatga
     1801 gcgcggcttc gaacgaccgt ttccgctcgg cttcgttggc ggctttcagc agcacggcat
     1861 tcttcttctc ggcttcgagc tcggcctgct tgcggttgag ttccctggct ttcgcttcct
     1921 gccgggcttt gtcgatcttc tcgagatgcg tcgtgatcga ggcataccag gtgcgcaatg
     1981 aatcgttatc ttcgtcatgc tgcagtgctc gctcgatttt ctcgaga
//
LOCUS       DAORHD010000990         2026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70505_length_2026_cov_2.990868, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000990 DAORHD010000000
VERSION     DAORHD010000990.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70505_length_2026_cov_2.990868"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /gene="pilM"
                     /locus_tag="PKO06_17860"
     CDS             complement(<1..1040)
                     /gene="pilM"
                     /locus_tag="PKO06_17860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HNV71575.1"
                     /translation="MGLFTFGASNTAVGVDIGTSSIKVVQLKKAPTGPELAGYAMGPI
                     PHGAVEDGQIKDAPTVAAVLKDMLKVSKIRPDRSFASISGQNVIMRFTKLPTMTPDEL
                     DQTVRIEAEQYVPYAIDEVSITHAVLQEVAEEEGGTKLSILLVVAQKELVNTYLDCMK
                     QGGVTPEIIDVDTLATINSLESSIMATASSQEGGEVVAIIDTGARTTNISVLKSGILM
                     FTRNIPIAGNNITTMIQNVMKQDTFDSAEQMKVSEAEVSISDSAGNEISEVVKTTVEE
                     LVSEIRRSFDYFKAQSREPLIHRIILSGGGSNLRNFDNYLSNEIGVDVARGNPLEGIN
                     VTVSDTDALFANL"
     gene            complement(1316..>2026)
                     /locus_tag="PKO06_17865"
     CDS             complement(1316..>2026)
                     /locus_tag="PKO06_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015205997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71576.1"
                     /translation="KDGTNYRWVRRLAVEFLRRRLATEKMPFTALKRVEVSMVKDRDR
                     ADRVRFDTDRGSYSMKATVFRRIFNLPSTYFWLQTAPVETPRPIEVPMKPVLAVAPPT
                     MPTVLPEELLTLLSGRRDGGPKQLVIQTAGGLRRASRPGEGWLILSARARPESLLVPT
                     KPVSEPATRGRLVGAPQPGVITVAKGGIREIALQGRGYGHQVGMCQAGAIAMGRLGWN
                     YRQILGYYFSRVALRRLP"
BASE COUNT          420 a          603 c          583 g          420 t
ORIGIN      
        1 aggttcgcaa acagggcatc cgtgtccgag accgtcacgt taatgccttc gagcgggttg
       61 ccgcgcgcca cgtcgacgcc gatttcgtta ctgagatagt tatcaaagtt ccgcagattc
      121 gagccaccgc ccgacaggat gatgcgatgg atcaagggtt cgcgggattg agccttgaag
      181 tagtcgaacg agcggcgaat ttcgctgacc agctcttcga ccgtggtctt gaccacttcc
      241 gagatctcgt taccagccga atcgctgatg ctcacttccg cttcagaaac cttcatctgt
      301 tcggcagaat caaacgtatc ctgcttcatg acgttctgaa tcatggtcgt gatgttgttg
      361 cctgcaatgg gaatattgcg ggtgaacatc agaatgccgc tcttgagcac ggaaatgttg
      421 gtcgtgcgcg cgccagtatc gatgatggcc acgacctcgc caccttcctg gctgctggcg
      481 gttgccatga tcgacgactc gagcgaattg atcgtcgcca gcgtgtcgac gtcgatgatt
      541 tccggggtga cgccgccctg cttcatgcag tcgaggtagg tgttcaccag ttctttctga
      601 gcgaccacca gaagaatgga cagcttggtg ccgccctcct cttcggccac ttcctgaaga
      661 acagcatgag taatgctgac ctcgtcgatg gcatagggaa catactgttc cgcttcgatg
      721 cgcaccgtct ggtcgagttc gtcgggggtc atcgtcggca gcttggtaaa gcgcatgatg
      781 acgttctgac ccgagatcga agcaaagctg cgatcgggac gaatcttcga gaccttgagc
      841 atgtccttca ggacagccgc gacggtgggc gcatccttga tctgcccgtc ttccacggcg
      901 ccatgtggaa tggggcccat ggcgtagccg gccagctcag ggccggtggg cgcctttttc
      961 agttggacca ccttgatgga gctggtgccg atatcgacac caaccgccgt gttactcgcg
     1021 ccaaatgtga acaatcccat atgttcgtgc ctccaggatc agttccgcgg accaggaccg
     1081 ttgggcagac ctgtgttgcg gcagcaacgt gagaggacct caccacccta tcggcacgat
     1141 gaaccggttt ttatagagat ctgcagaaaa aaaccggaat catgcacaaa ccggacagta
     1201 gcctgttccc atctctgaag ggtgtgaaga gccccgcccg ctgtcaattg gaacgaagcg
     1261 attctcggat ggatttaggc cgaaccaaag agcgagagac gccgacgggc atcggtcagg
     1321 gtagccgtcg caacgccacc ctactgaaat aatacccgag aatctgtcga taattccagc
     1381 ccagacgacc catggcaatg gcaccggcct gacacattcc cacctggtgt ccgtagccgc
     1441 ggccctgcag cgcgatctca cggattccgc ccttcgcgac tgtgataacg cctggttggg
     1501 gtgctccgac caggcgtcca cgggttgccg gctcgctgac gggcttggtg ggtacaagca
     1561 gcgattccgg acgggcacgg gccgagagaa tcagccaccc ctcacccgga cgcgatgcac
     1621 gtcgcaaccc gcccgcggtc tgaatcacca gttgcttcgg cccaccgtca cggcgcccgg
     1681 aaagcagggt cagcaattct tcgggcaaga cggtcggcat cgtcggcggg gccaccgcca
     1741 gtaccggttt catcggtacc tcgatcggac gtggagtttc gactggagcc gtctgcagcc
     1801 agaaataggt actcggcaga ttgaagatgc gtcggaacac agtcgctttc atggaatacg
     1861 atccgcggtc agtatcgaat ctgacccggt cggcccgatc gcggtctttc accatcgaca
     1921 cttcaacccg tttcaacgcg gtaaaaggca tcttctcggt cgccagtcga cggcgcagga
     1981 actcgaccgc cagacggcgc acccaccgat agttcgtgcc atcctt
//
LOCUS       DAORHD010000991         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70597_length_2024_cov_4.280345, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000991 DAORHD010000000
VERSION     DAORHD010000991.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70597_length_2024_cov_4.280345"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2024
                     /locus_tag="PKO06_17870"
     CDS             <1..>2024
                     /locus_tag="PKO06_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71577.1"
                     /translation="VGYLANQSYLEQQQIEQQRLEARQKCSDLIRRLQGQTTLTYLVE
                     DAVERLLRESYPSVTTARTPAELTFGAERFLTGLASIGFRVHRSALYATHRQPLGSGS
                     HNFSVCFRTGYAPEAFDGLDLLIAYATMEGSPFRQRVYEKIGGMNNSLGFPATPDQFY
                     YSYHGRVRPVLGSDGPAWFFWQKLSHSPMASSGARLGMPLSRQFVRPPGQPPSDGILL
                     TVFERGNEGQFFAPLLIAAHRDPEIALAVLPTDQKVPLYSSSFPDELKHLIGSDSRQT
                     IPSDWIVMQGELAQPYAARIITAVNVPVRRRWATLTLECLLLISFLGAACLVWYAALQ
                     HEAALGRSLSAQIWWGCLIGVLIPLTLAATLGTRFMAGEEGNERERRRQHLRHTLDTL
                     DERLIWHQNQLIASMTAWTSNPDFQRGVMNRKRDDLYHYYQQVNHLSQTTWPLFDLQE
                     ILCIYPHQESLTITSPGKPNLDAVFSRVLGLCYRDVIHVMNPTSVASATDQGTVQAAR
                     DEIVARAAIDIMRSIFGPDVYFQMLFGAGNPMLFAAGLGAIVVHHFLIPSPKSPVALA
                     AWFFDSRQTYAKVGYRLRGLVDDTTLQGACLTGGYGMQSLSFEHRPIPDLVLALRRTI
                     AAQRSQSLTISSGDEPLLMETRLGNHAADTIGWAVSPAAVVNRHIAQLRR"
BASE COUNT          391 a          622 c          541 g          470 t
ORIGIN      
        1 gttggttatc tcgccaatca gtcgtatctg gagcaacagc agatcgaaca acagcgattg
       61 gaagctcgac agaaatgctc ggatctgatt cgccggcttc aagggcagac caccctgacc
      121 tacctggtcg aagatgccgt cgaacgactg ctgcgtgagt cgtatccttc ggtcacgacg
      181 gcgagaacgc cagcagaact gactttcgga gccgagcgat ttctcaccgg cctggcttcc
      241 atcggctttc gcgttcaccg ctcggccctg tatgcgacac atcgacagcc tcttgggtct
      301 ggttcacaca atttttctgt ctgctttcgc acgggatacg cgccagaggc gtttgacgga
      361 ctcgatctgc tgatcgccta tgcgaccatg gaaggcagcc cgttcagaca gcgcgtctat
      421 gagaagatcg gcggcatgaa caacagcctg ggctttccgg caacgccaga tcagttctat
      481 tacagctatc acggacgtgt tcgaccggta ctgggttcag acggacccgc ctggtttttc
      541 tggcaaaaat tgtctcactc tccgatggcc tcgtccgggg cgcgtctggg gatgcctctg
      601 tcgcggcagt ttgttcgccc tccgggccag ccgccgtccg atggcattct gttgaccgtc
      661 tttgagcgcg ggaatgaagg acagttcttc gccccactgc tcattgccgc ccatcgtgat
      721 ccagagatag ctctggcggt tttgcccacc gaccagaaag taccattgta ttcttcctct
      781 tttccagacg aactcaaaca tctgatcggt tcagattccc gtcagacgat cccttccgac
      841 tggatcgtca tgcagggaga gttggcgcaa ccttatgcgg cacgcatcat cacagccgtg
      901 aatgtaccgg tgcgtcgtcg ttgggctacg ctgaccctgg aatgtcttct tctcatcagc
      961 tttctcgggg cagcctgtct cgtctggtat gctgctcttc agcacgaagc tgctctgggt
     1021 cgatctctgt cagcacagat ctggtgggga tgtctgatcg gggttctgat tcccctgacg
     1081 ctcgccgcca ctctcggtac gcgattcatg gccggagaag aaggaaatga gcgagaacga
     1141 cgacgacaac atctgcgtca caccctcgat acgctggacg aacgcctgat ctggcatcag
     1201 aaccaactca tcgcgtccat gaccgcctgg acttcgaatc cggattttca gcgtggcgtt
     1261 atgaatcgca aacgtgacga cttgtaccat tattatcagc aggtgaatca tctctcacaa
     1321 accacctggc ccctcttcga cctccaagag attctctgca tttatcctca tcaggagtct
     1381 ctcacaatca cgagtccggg aaaaccaaac ctggacgctg ttttctcgcg cgttctcggc
     1441 ctctgttacc gcgatgtgat tcacgtcatg aaccccacct ctgtggcatc ggctaccgat
     1501 cagggcaccg tgcaggcggc tcgcgacgaa atcgtcgcgc gtgccgccat cgatatcatg
     1561 cgatcaatct tcggcccgga tgtctatttc cagatgctgt tcggcgccgg caaccccatg
     1621 ctcttcgcag cggggttagg agcgatcgtc gtgcatcact ttctgattcc gtcaccgaaa
     1681 tcgccggttg cgctggcggc ctggtttttt gacagccgcc agacctacgc caaagtcggg
     1741 tatcgccttc gtggccttgt cgatgatacg accctgcagg gagcgtgcct gaccggtgga
     1801 tacggcatgc agagtctgtc gttcgaacat cggccgatcc ccgatctggt tctggccctg
     1861 cgccgcacca tcgccgcaca gcgttctcag agcctgacca tttcatcggg agacgagccg
     1921 ctgctcatgg aaacacgcct cggcaaccat gctgcggata caatcggctg ggcggtttca
     1981 cctgcggccg tcgtgaaccg ccatatcgcc cagctgcgtc gaca
//
LOCUS       DAORHD010000992         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70614_length_2024_cov_3.126968, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000992 DAORHD010000000
VERSION     DAORHD010000992.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70614_length_2024_cov_3.126968"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKO06_17875"
     CDS             complement(<1..561)
                     /locus_tag="PKO06_17875"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNV71578.1"
                     /translation="MAAKRLLDWYDQKGRDLPWRETSDAYRVLVSEIMAQQTQIARVL
                     PFFEHWLIRFPDWRRLAKAEVAEVIRAWSGLGYNRRALTLQNIAKQVVERGLPASETE
                     WLELKGVGPYTAAALTVFALGQRAVPIDTNIRRVTGRWLLGVPLPELSHDRHLRTVLA
                     PMVGRLKRFADFFQALFDLGATVCVKK"
     gene            666..1244
                     /locus_tag="PKO06_17880"
     CDS             666..1244
                     /locus_tag="PKO06_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV71579.1"
                     /translation="MPRLLLFNKPFNVLCQFTGEGTKKTLADFIDVPKVYAAGRLDYD
                     SEGLLLLTDVGFLNHLITDPEHKLPKTYLVQVEGTPTDDAISKLCNGVLLKDGMTRPA
                     QARVLTAEPPLWPRQPPIRERRHIPTTWLELTISEGRNRQVRRMTAAVGLPTLRLVRT
                     TIGPWRLDDLPPGTWRDSPCPRTPTEYSTFVR"
     gene            complement(1830..>2024)
                     /locus_tag="PKO06_17885"
     CDS             complement(1830..>2024)
                     /locus_tag="PKO06_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71580.1"
                     /translation="RVKAILTTSENLGVTTGRWCQRPTIDVFVILGHDEVPDQALVDK
                     LRPGKHLRDRQVSGKPVCGG"
BASE COUNT          437 a          667 c          530 g          390 t
ORIGIN      
        1 tttcttcaca caaacggtcg cgccaagatc gaagagggcc tgaaagaagt cggcgaaccg
       61 tttcaaccgg ccgaccatcg gggccagaac tgtgcgaagg tgtcgatcgt gtgacagttc
      121 gggcagcggg actccaagca accaccgccc ggtgacccgg cgaatgttgg tgtcgatcgg
      181 cacagccctc tgccccagcg cgaagaccgt cagtgccgcc gccgtgtatg gaccaactcc
      241 cttgagttcg agccattcgg tttcgctcgc gggaagaccg cgttcaacca cctgtttcgc
      301 aatgttctgc agggtcaacg cccggcggtt ataccccagg cccgaccagg cccgtatgac
      361 ctcggccact tctgctttgg cgagacgtcg ccagtcggga aaacgaatca gccagtgttc
      421 gaagaagggc aatacgcggg caatctgcgt ctgctgcgcc ataatctcgc tgaccagcac
      481 ccgataggca tcagaagttt cgcgccacgg aagatcgcga cccttctggt cataccagtc
      541 cagtaagcgc ttcgcagcca cctcggcctg tcttccggac ggaacttcag cgttacccac
      601 gggtgatgcc gttggtctgg tgttcctctt catgcgagaa gaatggtatc atacccgtca
      661 actctatgcc gcgactgctt ctcttcaaca aacccttcaa cgtgctatgc cagttcaccg
      721 gcgaaggcac aaagaaaacg ctggccgatt ttattgatgt accgaaggtc tacgctgccg
      781 gcagactgga ctatgacagc gaaggtctgc tgctgctgac ggacgtcgga tttctcaacc
      841 acctgatcac cgacccggag cacaaactgc ccaagaccta tctcgtacaa gtcgagggaa
      901 cgccaaccga tgacgctatc agcaaactgt gcaacggggt tttgctgaaa gacggcatga
      961 cccggcccgc gcaggcacga gtgctcaccg cagagccgcc tctctggccg cgccagccgc
     1021 cgatccgtga acgccggcac atcccgacca cctggctcga actcacaatc tcggaaggac
     1081 gcaaccgaca ggtgcggcgc atgaccgcgg cagtgggcct gccaaccttg cgactggtac
     1141 gtaccaccat cggcccctgg cggctcgacg acctgccacc cggcacctgg cgcgactccc
     1201 cctgcccccg cacccccact gaatacagta ctttcgtgcg ataagcctgg aacgaggcag
     1261 cggttgcctg gaaacacagt tcttactcgg cgggctattt tacttctccc ctgaagtacg
     1321 atcaggtcat tccaccgtgc catgcaacga gcaagaaatg cggcccgtcg tgtcaatgtc
     1381 cgaaccttcg tatgtccccc ccggcaccag agaccgaaag gtcaggggca gccgaaggtt
     1441 gtccgcaaac cgttccatgc aggtctggac gaaccgattg gaaaaacgcg gagccatttc
     1501 tcccggacac tcaatgggca tcttcaaata ccctcgcgct cggagaattg cgatattacg
     1561 tgatgagaca gtcttcatca tatcggtatg atccatattg tacatttcga aggccccgag
     1621 caacacgcga atgttcgcat aacaccctcc ggtatacgcc ggtcccgatc ctttcctgaa
     1681 gcccccgacc acgccccgta tcggaaccag gaacagaagc agcagggcga tgattcccag
     1741 gcctgctaag atcggtccag ctgtctgcgg cctcatccac cacacctccg ccattctcga
     1801 attgtccgcg cccacgctgc gcaaccccat cagcctccgc agaccggctt cccggaaacc
     1861 tgcctgtctc tcagatgctt ccctggcctg agtttatcta cgagtgcctg atccgggact
     1921 tcatcatgcc cgagaatcac gaacacgtca atggtgggcc gctggcacca ccgacctgtc
     1981 gtgaccccga ggttttctga agtcgtgagg attgctttga ccct
//
LOCUS       DAORHD010000993         2021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70786_length_2021_cov_2.672431, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000993 DAORHD010000000
VERSION     DAORHD010000993.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70786_length_2021_cov_2.672431"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PKO06_17890"
     CDS             <1..745
                     /locus_tag="PKO06_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNV71581.1"
                     /translation="KVLVWVSVLGSLMALFLGMAQGLVNLEKGMQAWVNGARSMLLAM
                     CVLVLAWAINGVCKDLHTADFIVALVRNILTPWLVPAIIFLVSCAISFATGTSWGTMA
                     IMLPISIPLAYHLNLDAFRANPAAYAGWTELAYVKTMMLVSIGAVLEGSIFGDHCSPI
                     SDTTIMSSMASGSDHIDHVRTQAPYAVAVAVVSLLVCYIPAGCGFNPFVLIPCAMVAL
                     VIVLNVLGSSVMDAQHPASQPTVVPTSKA"
     gene            756..1337
                     /locus_tag="PKO06_17895"
     CDS             756..1337
                     /locus_tag="PKO06_17895"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNV71582.1"
                     /translation="MSVLVHACCGPCLGGAAPELRRQHESLLLFWENPNIHPYLEYRA
                     RLESFHAMAEKLQLPVMHGTVDYGLGLFLRALNSEFGPARCAICYRIRLERTARRAHE
                     LGCEGFTTTLTISPYQNHEVICRVGAEVAAECSVPFVYHDLRPFFRGTHQAARENNLY
                     KQKYCGCLFSEYDRYQHDPKFALPECLDDPHES"
     gene            1339..>2021
                     /locus_tag="PKO06_17900"
     CDS             1339..>2021
                     /locus_tag="PKO06_17900"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HNV71583.1"
                     /translation="MLRFLRFLSVVLLLFLGLSERATAEIEMKVRVADQVRRLEVKLL
                     AGGDLFDERNRRVKTLRPNESFVWEVQAETKREEPKKPADKGKKDRQTRRPPARRKAD
                     PWLGKTIRFVSRRGQVTVNRLSYHGYLQVTFRSSGALVVNQLKLDDYIRGVVGREMGS
                     LSPLESLKAQAVMARTWAVANRGKHGKDGFDVCNAEHCQVYGGMAAERATTTAAVAAT
                     RGVIMTAEG"
BASE COUNT          379 a          579 c          617 g          446 t
ORIGIN      
        1 caaggtgctc gtctgggtct cggtgctggg atcattgatg gcactgtttc ttggcatggc
       61 tcaggggctg gtgaatctgg aaaaggggat gcaggcgtgg gtgaacggcg cgcgctcgat
      121 gttgctggcc atgtgcgtgc tggtgctggc ctgggccatc aatggggtct gcaaagattt
      181 gcataccgcc gacttcattg tcgctctggt acgcaatatt ctgactccat ggctggtacc
      241 cgcgatcatt ttcctcgtct cctgcgcgat ctcgtttgcg accggaacca gttggggcac
      301 catggcgatc atgttaccca tctccattcc gctggcgtac catctgaatc tcgatgcatt
      361 ccgcgccaac ccagctgcct atgctggctg gaccgagctc gcttacgtga agacgatgat
      421 gctcgtgtcg atcggcgcgg tgcttgaagg ttcgatcttc ggcgatcact gcagcccgat
      481 ctctgacacc acgatcatgt cgtcgatggc atcgggcagc gatcatatcg atcacgtgcg
      541 cacgcaggcc ccatatgcgg ttgccgtcgc ggtcgtctcc ttgctggtct gttacattcc
      601 ggccgggtgt ggattcaatc cctttgtgct gattccctgc gccatggtgg ccctggtgat
      661 tgtgctgaat gttctgggct cctcggtcat ggatgcgcag cacccagcgt ctcagccgac
      721 cgtggtcccg acttcgaaag cctgagcgtg gaaacatgag cgttctggtg catgcgtgct
      781 gtggtccgtg tcttgggggg gccgctcccg agctgcggcg gcagcacgaa tccctgctcc
      841 tgttctggga aaaccccaac attcatccgt atctcgagta tcgggcccgc cttgagtcgt
      901 ttcatgccat ggccgagaag ctgcaattgc cagtaatgca cggaacggtc gactacggac
      961 tcggactctt cctgcgggca ctgaattccg aattcggtcc ggcgcgttgt gcgatctgct
     1021 accgcatacg tctcgagcgc accgcccggc gcgcgcatga actggggtgc gaagggttca
     1081 ccaccacact gacgatcagc ccgtatcaga accacgaagt catttgccgc gtcggcgccg
     1141 aagtggccgc cgagtgctcg gtaccgtttg tgtatcatga cctgcgcccg ttctttcgcg
     1201 gcacccatca ggcggcgcgc gaaaataact tgtacaaaca gaaatactgt ggctgcctct
     1261 tcagtgaata tgaccgctat cagcatgatc cgaaatttgc tctgccggag tgcctcgatg
     1321 accctcatga atcatagcat gctccgcttt ttgcgctttc tgtccgtcgt tcttctcctg
     1381 ttcctcgggc tgagcgagcg cgcgaccgcc gagatcgaaa tgaaagtacg agttgctgat
     1441 caagtgcggc gcctggaagt caaacttctg gcgggtggcg atctctttga cgagcgcaac
     1501 cggcgagtca agacgttgcg acccaacgag agcttcgtct gggaagtcca ggctgaaaca
     1561 aagcgagaag agccgaagaa gccggcagac aagggaaaga aagaccgcca gacgcgtcgc
     1621 ccgccggcgc gcaggaaggc tgatccgtgg ctggggaaga cgattcggtt cgtctcgcgt
     1681 cgcggtcaag tcacggtgaa tcgtttgtcc taccacggat accttcaggt gaccttcaga
     1741 agttcaggag cgctcgtcgt gaaccaactg aaactggatg actacatccg cggcgtcgtt
     1801 gggcgagaaa tgggctcgct gtcaccgctc gaatcactca aggcgcaggc tgtcatggcg
     1861 cgcacctggg cggtggccaa ccgtgggaaa cacggcaagg atggtttcga tgtctgtaat
     1921 gccgaacact gccaggttta cggagggatg gccgcggaac gggcaacgac gaccgccgcc
     1981 gtggcggcga ctcgtggagt gatcatgacc gccgaaggtt t
//
LOCUS       DAORHD010000994         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70939_length_2018_cov_3.684666, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000994 DAORHD010000000
VERSION     DAORHD010000994.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70939_length_2018_cov_3.684666"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PKO06_17905"
     CDS             <1..1485
                     /locus_tag="PKO06_17905"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV71584.1"
                     /translation="LLGRLLEVGCAADRPNNFGMTPLHLACKKGESRMVARLLEAGSN
                     AMARDRYHDTPLHDAVQNDEGEIVRLLLEHLETPDVADKFGTTPLMRAAEMGNVRVLE
                     ALLRGGADASRSDKFGVTALHKAVAGKNTAAIRLLARTRLAVNQPEAKGNTPLHLVAR
                     SGDVEIARILLAEGADPDIPDAQNMTPIHCAASCGHTELQRLLARAPAVSDSPQKPER
                     EDLPPLPPIPDSPADADVSFEKIIHLEGRQLKRLLQSVDQQDLVKALHQESEEVRQRF
                     CKLLGGPAAEAFRGTLKLEIAYSESDIQESRRRISQKLRSLVEKGSIKLRFERHGAME
                     KLARAIEKPPSAPSSAGLFDSILGLFGSGEAALIEELHTAVKGGSFYRTEKLLNEHPQ
                     LLNAPDLAFYQETPLHKAARYGHLDIAQLLVQRGADVRVQNDMGITPLHKAAFWGHET
                     VAELLLDVGADPNARDQIGETPLKKAVEGKHPRIVELLKNRGGH"
     gene            1529..>2018
                     /locus_tag="PKO06_17910"
     CDS             1529..>2018
                     /locus_tag="PKO06_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71585.1"
                     /translation="MGCLKRFLLCGCAGTLALVFLAGGILGLLAQSLRQNSLQARSAP
                     VKTLFEALNSDGPAWVRVRLEPASASEVLRCGETPAVWVSFQGRELVTQEIRRGGAWR
                     MRSVWKKPAVEARTVPVLLKTDAAQFLLTDWLGVEPWPDLFQRRRITDYLNAENGPRL
                     AEE"
BASE COUNT          397 a          570 c          637 g          414 t
ORIGIN      
        1 ctgctggggc ggctcctcga agttgggtgt gccgctgacc gcccgaacaa cttcggtatg
       61 actccgctgc atctggcctg caagaagggc gaaagccgca tggtggctcg tctgctcgag
      121 gccggatcaa atgcgatggc tcgcgatcgc tatcatgaca ctcccttgca cgatgcggtg
      181 caaaacgatg agggagagat tgtgcggctg ctgctcgaac atctcgagac gcccgatgtc
      241 gccgacaagt ttggtaccac gccgctgatg cgagctgccg agatgggaaa tgtccgagtt
      301 cttgaagcac ttctgagggg cggagccgat gcgagccggt ctgacaaatt cggcgtgacg
      361 gcgctgcaca aagcggttgc cgggaaaaac acggcggcga tcagacttct ggcgcgaacg
      421 cgtctggccg tgaaccaacc cgaggccaag gggaacacgc ctctgcatct cgtggcgcgc
      481 agcggagatg tcgagattgc ccgcattctg ctcgctgagg gagcagaccc ggatatacca
      541 gatgcccaga acatgacgcc gattcattgt gccgcgtcgt gtggacacac cgaactgcag
      601 agactgctgg ccagggctcc cgcggtcagt gattcgccac agaagccgga gcgtgaagat
      661 ctcccgccgc tgcctcccat tcctgattcc cctgctgatg ccgatgtctc cttcgagaag
      721 atcatccacc tcgaggggcg gcagctcaag cgtttgttgc agagcgtaga ccagcaagac
      781 ctcgtgaaag ctttgcacca ggagtcggag gaggtgcggc agcgtttctg caagctgctt
      841 ggcggcccgg ctgccgaggc ttttcgtgga acactgaagc tcgagatcgc gtattccgag
      901 tcggacattc aggagtcgcg acgacgcatc agtcagaagc tgcggtcgct ggttgagaag
      961 gggtcgatca aattgcgctt tgaacgtcat ggggccatgg aaaaactggc ccgggcgatc
     1021 gagaagccgc cttctgcgcc gtcgtcggcc ggcttgtttg attccattct tggtctgttt
     1081 gggtcgggcg aagccgctct gatcgaagaa ctgcatacgg cggtcaaggg tggttctttt
     1141 tatcgcaccg agaaactcct gaacgagcat ccgcaactgt tgaacgcccc ggatctcgct
     1201 ttttatcagg aaacaccgct acacaaggct gcccgctacg ggcatctcga tatcgcacaa
     1261 cttctggtgc agcgcggagc cgatgtgcgt gtacagaatg atatgggcat taccccgctg
     1321 cacaaagcgg ctttctgggg gcatgaaacg gtggcagaac tgctgctcga tgtgggtgcc
     1381 gacccgaatg cccgcgacca gatcggcgag acccccctca agaaagcagt cgaaggcaag
     1441 catccgcgca ttgtcgaact gctgaagaac cggggcggac actgatggtg aatcggtcga
     1501 tgacatctct gctggccggg tttccgacat gggttgcctg aaacggttct tgctgtgcgg
     1561 ttgcgcggga accctggctc tggtgtttct ggctggcgga attctcggtc tgctggcaca
     1621 aagtttacgc cagaacagtt tgcaggcgcg ctcggcccct gtgaagactc tgtttgaagc
     1681 actgaactcc gatggtccgg cctgggttcg tgtgcgtctt gaacccgcct cagcgagcga
     1741 agtgttacgc tgcggcgaga cgccggccgt gtgggtgtct tttcagggac gtgaactggt
     1801 aacccaggag attcgccgtg gaggcgcctg gcgcatgcgg tcggtctgga aaaagcctgc
     1861 cgttgaagca cgaactgtcc ccgttctgtt gaagaccgat gccgctcagt ttctgttgac
     1921 cgactggctc ggagtcgaac cctggcctga tctctttcag cgtcgccgta tcaccgacta
     1981 tctcaatgcc gaaaatggcc cgcgcctggc ggaagagt
//
LOCUS       DAORHD010000995         2018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_70940_length_2018_cov_3.423841, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000995 DAORHD010000000
VERSION     DAORHD010000995.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70940_length_2018_cov_3.423841"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKO06_17915"
     CDS             complement(<1..463)
                     /locus_tag="PKO06_17915"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNV71586.1"
                     /translation="MTHVSRSTLLAFLEETFKTSVTPDVSYNGLQFEGSQNIASIVTG
                     VDASCEFFRQAARRKADFAIVHHGLFWKGGEWKRITRFEKEKMELLLKGNFSLYALHL
                     PLDSHPDLGNNAVMAQLLGLENLEPFSEYQGQKIGIKGRFPRPISRAQLQTR"
     gene            complement(460..1260)
                     /locus_tag="PKO06_17920"
     CDS             complement(460..1260)
                     /locus_tag="PKO06_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71587.1"
                     /translation="MFSLFRVCLALCLLLFTQSSLYGFEIHLGPWNDRLLDLSSGTLL
                     LVDPDDVSTKQLQQLHSRGVRPLAWLNLGYREPGRSFTAHVEDRSLSLSGKKPRQSRY
                     PVRFFSALWKRLLQQRIQEITNLGFRGLFLTGLQAATQITDHPIAVEEMMNLCRWIRH
                     LHVGLVDHPFLVIDMPLNFRVPATSLSFTDYICLQGIWYTSPRRSIRPWERQPHLDRW
                     FRQQQIPLLTIDNAPAAKQPDLRAEVAALGADAGFATLPLYPIRSRLP"
     gene            complement(1314..>2018)
                     /locus_tag="PKO06_17925"
     CDS             complement(1314..>2018)
                     /locus_tag="PKO06_17925"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNV71588.1"
                     /translation="PSPARASAPDADERLTFVLPEEANAATVFLRLGYDIQLTPWLVS
                     RGQFLKERAPDLDSLQRMPGLSLDDPLLEQLHLALKGVPGNGLQNLYLAVSRGLSYKE
                     NFEDHPVSWALENPHLCDCTEYSYLLAALCLKRSIPARVVTGFLIKPDLLDRETNVGH
                     AWVETYFPDKGWVPVDPTLAANMRWAYFGNLLSDQILFERPTVDAKARVSVDVTSTRN
                     DLSLKISSSFLCSLIN"
BASE COUNT          462 a          509 c          637 g          410 t
ORIGIN      
        1 tgcgggtctg cagttgagct cgactgatcg gtcggggaaa ccgtcctttg atgccgatct
       61 tctgcccctg atactctgaa aacggctcca ggttctcgag acccagcagc tgtgccatga
      121 ccgcgttgtt tccgagatcc ggatgactgt cgaggggcag gtgcagggca tacagactga
      181 agttgccctt gagcagcagc tccattttct ctttttcaaa gcgggtgatg cgcttccact
      241 cgccgccctt ccagaaaagt ccatgatgga cgatggcaaa gtctgccttg cgacgtgcgg
      301 cttgccgaaa gaactcgcag ctggcatcga cgccggtgac gatgctggcg atgttctgac
      361 tgccttcaaa ctgcagcccg ttgtaggaaa cgtcgggtgt gacactggtc ttgaatgttt
      421 cttccagaaa cgccagcagg gtggatcgac tcacatgggt catggtaaac ggctcctgat
      481 cggataaagt gggagagtgg cgaaaccggc gtcggctcca agagcggcaa cctccgctcg
      541 caggtccggt tgctttgccg ccggagcatt gtcgatcgtg agaagaggaa tctgttgctg
      601 acggaaccag cggtcgaggt ggggttgacg ctcccaggga cgaattgatc gtcgaggact
      661 ggtataccag atcccttgca ggcagatgta atcagtaaat gataacgatg tggctgggac
      721 tcgaaaattc agcggcatgt cgataacgag aaacgggtga tcaaccagac cgacgtgcag
      781 atgacgaatc catcggcaca ggttcatcat ctcttcgacc gctatgggat gatccgtaat
      841 ctgcgtggct gcctgcagac cagtgagaaa cagtccgcga aatcccaggt tggtgatctc
      901 ctgaatccgt tgctgcagca ggcgtttcca gagcgcggaa aagaatcgga ccgggtagcg
      961 agactgtcgt ggcttcttcc cgctcaggct caatgaccgg tcttccacgt gagcggtgaa
     1021 actgcgcccg ggttctcgat aaccgaggtt gagccaggcg agcggtcgaa cgcctctcga
     1081 gtgtaactgc tgcaattgct ttgtcgaaac gtcgtcggga tcgacgagca gcagcgtgcc
     1141 cgaagagagg tcgaggagac ggtcgttcca ggggccgaga tgaatttcga aaccgtacag
     1201 ggaactctgc gtgaagagaa gcaaacagag cgcaagacag acccgaaaga gtgaaaacat
     1261 cgaacgaagc gtggccgagg ggagagagtg agtcatagac aatcgcttgt gtcctagttg
     1321 atgagactgc agagaaaact ggaagaaatc ttcaaggaca ggtcgttccg tgtcgaggtg
     1381 acatcgacgc tgacccgggc tttggcgtcg accgtggggc gttcgaacag gatctgatca
     1441 gacaggaggt tgccgaaata ggcccagcgc atgtttgcgg ccagggtcgg atccaccggg
     1501 acccatccct tgtccggaaa atatgtctcg acccaggcgt gaccgacatt ggtttcgcga
     1561 tcgagcaggt cgggcttgat gagaaagccg gtaacgacac gggcgggaat acttcgcttc
     1621 agacacaggg ccgccagaag gtatgaatat tcggtgcagt cacacagatg cgggttctcc
     1681 agtgcccacg aaaccgggtg atcctcgaag ttttccttgt atgaaagccc gcgactgacg
     1741 gcgagataga gattctgcaa accgttgcct ggtactccct tcaatgccag atgcagttgc
     1801 tccagaagcg ggtcgtcgag tgaaaggccg ggcatgcgct gcagtgaatc gagatcgggt
     1861 gctcgttctt tcagaaactg acctcgactg acgagccagg gagtcagttg tatgtcgtat
     1921 cccaggcgga gaaagacggt ggcggcattc gcttcttcgg ggagaacgaa ggtcaggcgt
     1981 tcgtcagcgt cgggcgcaga agcgcgtgcc ggagaggg
//
LOCUS       DAORHD010000996         2017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71019_length_2017_cov_2.851172, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000996 DAORHD010000000
VERSION     DAORHD010000996.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71019_length_2017_cov_2.851172"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKO06_17930"
     CDS             <1..453
                     /locus_tag="PKO06_17930"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanoyl-CoA acyltransferase"
                     /protein_id="HNV71589.1"
                     /translation="HSAEYLPLSKRPNKHTLEAGRNAVEKAYKEAGIKAKNVDIAEVH
                     DCFTINQILTTEALGLSDHGKGGADYVAGRFTADDDQVCVNLSGGLKAKGHPVGATGA
                     SMHALVYKQLIGEPIGAKPTKKTPEVGVTFNVGGSAVTNCVTVLRRIK"
     gene            complement(527..>2017)
                     /locus_tag="PKO06_17935"
     CDS             complement(527..>2017)
                     /locus_tag="PKO06_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71590.1"
                     /translation="PTTPQPTTPATSAAPTPSSANPKLDELVRKIDTLIENLNPPTDP
                     APALSLVLQLAMEFQRSDLAAEKRNKLFDRYFGQAQSLMSKDRDRAAAVLRQCRAIAP
                     ERPEPAFALLDLQESGAVRTMSTDASTATAASPAAAKARAAVEKLLKDGKPKKLAEIS
                     KHLADLEKAGDAAGADVLRKLAIGNLRDRISSSTSVGEMQSLIPVCRSLIPEGTDERK
                     TFDTESETAVRDRKTKSIEETKKLVDNLEPPGNPKALAARIRGLKEIGEAELAKTLTK
                     SVKKSFMASIATAQADSPAKALSLVRSLQLFDGMKQDAEVTKLANALAVKVPEKATGT
                     NTGTTSGDDDAATTDTATTEDVALGADDDGEPVMTAPLGKTKKGKKAASGKPAKAASD
                     GEGEDTADGGSDDALIAELEQLISPDKIEDSIDAMLAAIKKLEKAGRKSEASEYRQRG
                     AVVLQEIADENAANGDKDRARVAYNKALKLSPGNKSVLAALEFVKE"
BASE COUNT          361 a          590 c          583 g          483 t
ORIGIN      
        1 cactcggccg agtatctgcc cctctcgaag cgtccgaaca aacacacgct cgaagccggt
       61 cgcaatgcgg ttgagaaggc atacaaagaa gcgggaatca aggccaagaa cgttgatatc
      121 gccgaagtgc atgactgctt caccatcaat cagattctga ccaccgaagc gctcggtctg
      181 tctgatcacg gaaaaggcgg cgctgactat gtagccggcc gcttcactgc tgatgacgat
      241 caggtgtgcg ttaacctgtc gggtgggttg aaagccaagg gtcacccggt cggcgcgacc
      301 ggagcgtcaa tgcacgccct ggtctacaag cagttgatcg gtgaacccat cggcgcgaag
      361 ccgaccaaga aaacccccga agtgggcgtg accttcaacg tcggcggctc agccgtcacc
      421 aactgcgtca cggttctacg ccgtatcaag taacctctcg atttatagta caagttcaac
      481 tcgctgattc tcggcttcaa ctccgagaat cagcgagttg tatttatcac tctttgacga
      541 actcgagggc cgcgagcacg gatttgttgc cggggctgag tttcagcgct ttgttgtagg
      601 cgactcgggc gcggtctttg tctccgttgg cggcattctc gtccgcgatc tcctgtaaga
      661 cgaccgctcc gcgttgtcga tactcactag cctcgctctt acgaccggcc ttctcgagct
      721 tcttgatcgc tgccagcatt gcgtcaatgc tgtcttcgat cttgtcgggc gagatcaatt
      781 gttcgagttc ggcgatgaga gcatcgtcgc tgccgccatc cgccgtgtct tccccttcac
      841 cgtcactcgc ggctttggct ggcttgcccg aagcggcttt cttgcctttc ttcgtcttgc
      901 ccagtggcgc agtcatgacg ggctcaccat catcgtcagc gccgagtgct acgtcttcag
      961 tcgttgcggt atcggtcgtc gccgcgtcgt cgtctccact ggtagttccg gtattcgttc
     1021 cagtcgcttt ttctggcact ttgaccgcaa gagcatttgc caacttggtt acctcggcat
     1081 cctgcttcat gccgtcaaag agttgcagcg accgcaccag ggaaagcgct ttcgccgggc
     1141 tgtcagcctg cgccgtggca atcgaagcca tgaaactctt tttcacgctc ttggtcagtg
     1201 tctttgccag ttcagcctcg ccgatttcct tcagaccacg gatgcgagcg gccagggctt
     1261 tcggattccc cggaggttcg agattgtcga ccagcttctt cgtctcttcg atcgacttgg
     1321 ttttacgatc gcgcaccgcg gtttccgatt cggtatcgaa ggtcttgcgc tcgtcggtgc
     1381 cttccggaat caggcttcga cacacaggga tgaggctctg catttctccg acagaggtcg
     1441 aggaactgat gcgatcgcgc agattgccaa tcgccagctt gcgcaggaca tcggcacccg
     1501 cggcatcgcc ggctttttcg agatcggcga ggtgcttcga aatctcggcc agcttcttgg
     1561 gttttccgtc tttcaacagc ttttcgacgg ccgcccttgc ctttgcggca gcaggtgatg
     1621 ccgctgtcgc cgtggaagca tctgtcgaca tggtgcgcac ggcacccgat tcctgaagat
     1681 cgagcagagc gaaagccggc tcaggtcgtt cgggggcaat ggcacgacac tggcgcaata
     1741 ccgcggcagc gcggtcgcgg tctttcgaca tcaggctctg ggcctgaccg aagtaccggt
     1801 cgaacagctt gttccgcttc tcggcggcga gatcgctgcg ctgaaactcc atggccagct
     1861 gcagtaccag tgacaaagcc ggtgccgggt cagtgggggg attcagattt tcgatcaggg
     1921 tatcgatctt acgtacaagt tcatcgagtt tcggattcgc tgatgaagga gttggagcag
     1981 ctgacgttgc cggcgtggtt ggttgcgggg tggtcgg
//
LOCUS       DAORHD010000997         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71117_length_2015_cov_3.897959, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000997 DAORHD010000000
VERSION     DAORHD010000997.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71117_length_2015_cov_3.897959"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..>2015)
                     /locus_tag="PKO06_17940"
     CDS             complement(43..>2015)
                     /locus_tag="PKO06_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71591.1"
                     /translation="TPSTTMPPALTTVPATQATPAPTPVAAPLVTPVAPAATAQTPSP
                     TGSTSGLSIERRFPNGGGYADPYKPVEVHFSGDLPADTVPEKVVRVTTLNPAGQQVAV
                     DGRWLKVGARAIHFLPTRYQPGAVYHVRVSAPPAVTEEVFRFAVLPDVKMTVDDSGAE
                     LQIFLTWNELRDLYPAADGQVTRLEFAEISISSGGKPCWNLKLDDSLPPVGEVNDAIF
                     QGEPFRFAVRVPKARLTTADGVFEVALDAAFAGRDKLLRFRKSTLKTIDSAIGSAKPE
                     PLTASQMPQPPVASTPSAIAPVAQPAVPFTEPAAAKPGTENPAETASATKRPGTLAPL
                     GPIPLDNTTEPDLAAAKPEPVTPPVTPVVATLKPVTPIADPGPQAIPVPVRDISLVEK
                     DSTSHSWPKGMRWAADGALWVVDSQGRRVMRFSEDGALLGQFGVRGEKPTKAGGLRNP
                     VDVVVSAAGVFVSDTSAHCVHLFHPDGRLIRAIGTWGTKRGQMDLPHGIDFNQEQLWI
                     ADRGNSQIMRFGSDGTFQGAFGAKSDLPGGLNNPISVCVTADGCWVLEGENGRVQKFD
                     RTGRLKFHFLSGAKDPTYLAVDPWGYLWLADGEDHQVKRFDNRGKAIVSIEPRLGTRP
                     WVPTSVAVRADGLIAVGDAENRLIRLFRLQKP"
BASE COUNT          409 a          618 c          589 g          399 t
ORIGIN      
        1 cgctgttcgc aaagctgccc gcgatcatca tgtttcgagg gatcagggtt tctgcagtcg
       61 gaaaagacgg atcagtcgat tttccgcgtc gccaacggcg atcaggccat cggctcggac
      121 tgccaccgat gtcggcaccc agggtcgggt tccgagtcgt ggctcaatgc tgacgatcgc
      181 ttttccgcgg ttgtcaaagc gtttcacctg atggtcttct ccatccgcca gccagagata
      241 cccccatggg tccaccgcga gatacgtcgg atctttggct ccgctcagga aatgaaactt
      301 caaccggccg gttcgatcaa acttctgcac ccggccgttt tcaccttcga gaacccagca
      361 accatctgct gtgacacaga cgctgatcgg attgttcaat ccaccgggca gatcgctctt
      421 ggcaccgaaa gctccctgga acgtgccatc tgaaccgaat cgcatgatct ggctgttccc
      481 gcgatcggca atccagagtt gttcttgatt gaagtcgatt ccgtgcggca gatccatctg
      541 gcctcgtttc gtgccccagg taccgatagc ccgaatcagt cgaccatcag ggtggaacag
      601 atgcacacaa tgagccgagg tatcagaaac aaacacgccg gccgcactga caacgacatc
      661 gactggattc cgcaagccac ccgccttcgt tggtttttca cctcgtacgc cgaactgtcc
      721 aagcaacgct ccgtcttctg agaagcgcat cacgcgacgc ccctgactgt cgactaccca
      781 caaagctcca tcagcggccc agcgcatccc tttcggccat gaatgagaag tggaatcctt
      841 ctcgaccagg gaaatgtcac gtaccggtac ggggatcgcc tgcgggcctg gatcagcgat
      901 cggagtcacg ggtttcagcg tcgccaccac aggagtcacc ggcggcgtca cgggttcggg
      961 ctttgcggcc gcgagatccg gttcagtggt gttgtcaagc gggatcggcc caagcggggc
     1021 cagagtacca ggccgtttcg tagctgaagc cgtctcagcg ggattttccg tgccaggctt
     1081 tgccgcggca ggttcggtga acggaaccgc gggctgggca acgggcgcga tagcagacgg
     1141 agtcgacgca accggaggct gaggcatctg gctcgcggtc aacggttcgg gtttcgcgct
     1201 gccaatagcg ctatcgatcg tcttcaatgt gcttttgcga aaacgcagca gtttgtcacg
     1261 cccagcaaag gcagcatcaa gcgcaacctc aaacacgcca tcggcggtcg tcaggcgggc
     1321 tttaggaacc cgcacggcaa acctgaaggg ctcgccctga aagatggcgt cgttgacttc
     1381 accgaccggc ggcagcgaat catccagttt gagattccag cagggctttc caccgctact
     1441 gatactgatt tcagcgaatt ccaacctggt cacttgcccg tccgcggctg gatacagatc
     1501 gcgcagctcg ttccatgtca ggaaaatctg caactcagcg cctgaatcat cgacggtcat
     1561 cttgacgtct ggcagaacgg caaatcgaaa cacttcttcc gttacggctg gcggagccga
     1621 gacgcgcaca tggtagaccg ctcccggctg gtaccgggtg ggcaggaagt gaatcgcccg
     1681 cgccccgact ttcagccagc gtccatcgac tgcgacctgc tggcccgcag ggttgagcgt
     1741 ggtcacacgc accacttttt ccggaaccgt gtccgccgga agatcaccgg aaaagtgcac
     1801 ttcgacaggt ttgtacggat cggcataccc gccaccattc ggaaaccgtc gttcgatcga
     1861 cagaccggat gtcgatcctg tcggtgacgg agtctgggct gttgccgcgg gagccaccgg
     1921 agtgacgagc ggagcggcaa ccggagtcgg agcaggagtc gcctgcgtgg ccggcactgt
     1981 tgtcagtgcc ggcggcatcg tcgtcgaagg cgtct
//
LOCUS       DAORHD010000998         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71242_length_2013_cov_4.070991, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000998 DAORHD010000000
VERSION     DAORHD010000998.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71242_length_2013_cov_4.070991"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PKO06_17945"
     CDS             complement(<1..649)
                     /locus_tag="PKO06_17945"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71592.1"
                     /translation="MKINTLVFENLNSLKGRFQIDFNDPQLAGSGIFAISGPTGSGKT
                     TILDAITLALFASTARLKRAINQSSNELMTRGTAHCRAEVEFEATVPDGRRLTFRSYW
                     EQRRARNRPDGNLQPARMGLMQVSDKCPGDTFDCQQLTKVPGVVEQITGLDFNRFTRT
                     CLLAQGQFAAFLEADDRDRAEILEQITGTEVYARISQAVFDRDKLEREKITLLEAQ"
     gene            complement(646..1872)
                     /locus_tag="PKO06_17950"
     CDS             complement(646..1872)
                     /locus_tag="PKO06_17950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HNV71593.1"
                     /translation="MKILHTSDWHLGQTLIDVKRDEEHRAFVAWLLDIVREHRIEVVL
                     VAGDIFDNGMPGNQTLEIYYSFLAQAASAGCRQVIVVGGNHDSPSTLHAPRAILQRLN
                     VTVVGAVSTECPDALIVPLHDDAGTLSAVVCAVPFLRDRDVYLPKLGENDEERAKGIV
                     TGIAHYYEQVAERADNLRRQAGKPDAPIIATGHLFARGGITGEKERPIYIGNLGAFPA
                     ERFPKTFSYVALGHLHRPQLVENHARIRYSGSPLPLAFDEIGYDKQVIVFDTLAPADP
                     QPISVKTFRKLYTIAGDLPQIERQVREIASHTSSDEIKPLVQASYTGTELLPDLAEQV
                     SEFCENLPLRCFTLNRSPSKTVATEQQYTDIRQLTPHQVFERRLESETLSEADRKQVT
                     QAFDEIIAQLTTGEPS"
BASE COUNT          386 a          571 c          632 g          424 t
ORIGIN      
        1 gttgggcttc gagcagggtg atcttctctc gttccagctt gtcgcgatcg aagacggcct
       61 gggaaatgcg ggcgtagact tcggtaccgg tgatctgttc aagaatctcg gcgcggtcgc
      121 ggtcgtcggc ttcgagaaag gccgcgaact gtccctgcgc cagcagacag gtgcgggtga
      181 agcggttgaa atcgagaccg gtgatctgtt ccacaacgcc cggcactttc gtgagctgtt
      241 ggcaatcgaa ggtgtcgccg gggcatttgt cagaaacctg catcagaccc atgcgcgccg
      301 gttgcagatt gccgtcgggg cgattgcgcg cgcggcgctg ttcccaataa gagcgaaagg
      361 tcaggcggcg accgtcaggc accgtggcct cgaattcgac ttccgctcga cagtgcgccg
      421 tgccgcgcgt catcagctcg ttggagctct gattgatcgc acgtttgagt cgcgccgtgc
      481 tcgcgaagag cgcaagcgtg atcgcatcga gaatcgtcgt cttgcccgag ccggtcgggc
      541 ccgagatggc aaagataccg gagccggcca gctgagggtc gttgaaatcg atctgaaacc
      601 tgcccttgag cgaattgaga ttttcaaaga cgagggtatt gatcttcatg acggttctcc
      661 ggtggtcagc tgagcgatga tctcgtcgaa ggcctgcgtg acctgtttcc tgtctgcttc
      721 agacagcgtc tcgctctcaa ggcggcgctc gaagacctga tgcggggtca gctggcgaat
      781 atcggtgtac tgttgttcgg tggcgacggt cttcgacggc gatcggttca gggtaaagca
      841 ccgcagaggc agattctcgc agaactcgga cacctgttcg gccagatcgg gcagaagttc
      901 cgtaccggta taggaagcct gaaccagcgg cttgatctcg tctgacgagg tgtgagaagc
      961 aatttcgcgc acctgtcgct cgatctgcgg cagatcaccg gcgatcgtgt acagtttccg
     1021 gaaggtcttg accgagatcg gttgcgggtc ggccggcgcc agagtatcga acacgatcac
     1081 ctgcttgtca tacccgattt cgtcgaaggc caacggcaga ggggaaccgc tgtagcggat
     1141 ccgcgcgtgg ttctcgacca actgcggccg gtgcaggtgc ccgagagcga cgtaagaaaa
     1201 cgttttcgga aaccgctcgg cggggaaggc cccgaggttg ccgatgtaga tcggacgttc
     1261 tttttcgccg gtgatgccgc cgcgcgcgaa cagatgcccg gtggcgatga tcggcgcatc
     1321 gggttttccc gcttgccgcc gcaggttgtc ggcccgctca gcgacctgtt catagtagtg
     1381 cgcgatgccg gtaacgatgc cttttgcccg ttcctcgtcg ttttcaccca gtttcggcag
     1441 atacacgtcg cggtcgcgta gaaacggtac ggcgcagacg acggcggaaa gcgttccggc
     1501 gtcatcatgc agtgggacga tcagagcgtc aggacactcc gtcgagacgg ccccgacgac
     1561 ggtcacgttc aaccgctgaa gaatggcccg cggcgcgtgc agggtcgagg gggaatcgtg
     1621 gttgccgccg acgacgatca cctggcggca tccggcagag gcggcctgag ccagaaacga
     1681 gtagtagatc tcgagtgtct gattgccggg catgccgttg tcgaagatgt ctccggcgac
     1741 aagcaccacc tcgatccggt gctcgcgcac gatgtcgaga agccaggcca caaaggcccg
     1801 gtgttcttcg tcacgtttga cgtcgatcaa cgtttgtccg agatgccagt ctgaggtatg
     1861 cagaatcttc acccaagctc ctccttggct acacgggatg ccaccacgca caaccatatt
     1921 ccggaaattt ccagaatgca attccgcatc ctcgattaca gggtcattta attctcctcc
     1981 ttacttattt tccgttcaca tcgctcaacg att
//
LOCUS       DAORHD010000999         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71304_length_2012_cov_3.108840, whole genome
            shotgun sequence.
ACCESSION   DAORHD010000999 DAORHD010000000
VERSION     DAORHD010000999.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71304_length_2012_cov_3.108840"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..>2012
                     /locus_tag="PKO06_17955"
     CDS             24..>2012
                     /locus_tag="PKO06_17955"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNV71594.1"
                     /translation="MSRSYFENSDASDFRHHELFIEHFYKNERKKCLLRYQGEINDFI
                     FHDFDIPTLLEQIESITWACRDNNVMYRTFANRDAGFLLHSIYVDEILGNRASEFKIP
                     AFASSRASFCDERGLIKRHLVIDYDDDAQTFEIRCINVAHKTLLTNLWETIVLHNLNV
                     DFVEVWRKLHETEKVNSFHVVRIHSADRIEAEEVQSLTSDFERYIQKYTIPMSIFDLV
                     GPAMVGPSSSHTAGANRIGQIARNVILARVERGETIQSLEIKLMGSFRDTGLGHKTPL
                     ALAGGLQGLTADDETMLSHGEPEYLRTNGFVFGAGKTRFIGFTRGLPEDDLRYAGEKC
                     NNVAEIIFTTESGTHFITGFSIGAGNVEIRYVDTLLETPITGKRDFYLDGDTIVATQN
                     AAGTLPVIRGIFDRNEPETGYVMRFNTLEELIGYCRRERKRLVDVVIDVESGMYAMTR
                     EDIFQKMRIYWRAMENSVKTGIAGRKMSMLGLSGDSGFKLNGYAAGHPLFDNIYGRAV
                     AYATAVNEVNARSGVIVACPTAGSCGILPGVLRAYSELANPPEERILESLLVSGFLGM
                     LLFTDVSMAGADHGCQAEVGSAAAMAAAALCYQEGGDVEQVIAAFTLAIKNSLGLICD
                     PIAGLVEVPCVKRNGVYSSLAISAAMMALSGIKSYVSPD"
BASE COUNT          458 a          533 c          558 g          463 t
ORIGIN      
        1 atcccgagca gttttgcgcc gttatgtcca gatcgtactt tgaaaactct gatgccagtg
       61 atttcaggca tcacgaactc ttcattgaac acttttacaa aaatgaacgt aagaagtgtc
      121 tgctgcggta ccagggggaa atcaacgact tcatttttca cgacttcgat attccgacgt
      181 tactggagca gatcgagagc atcacctggg cctgccgcga caacaacgtt atgtatcgca
      241 cgttcgcaaa ccgggacgcg ggctttctgc ttcactcgat ctatgtagac gaaatcctcg
      301 gaaacagagc ctcggaattc aagattcccg cattcgcttc atccagggct tctttttgcg
      361 atgagcgggg cctgatcaaa cggcatctgg tcatcgacta tgacgatgat gcgcagacct
      421 tcgagattcg atgcatcaac gtcgcccaca agacgttgct caccaatctc tgggagacga
      481 tcgtgttgca taatctgaac gtcgattttg ttgaagtctg gcgaaaactg catgaaaccg
      541 agaaggtgaa ttccttccat gtcgtcagaa tccacagcgc tgaccgcatc gaagcagagg
      601 aagtgcaatc gctgacatcg gatttcgaac gctatattca gaagtatacc attccgatga
      661 gtatcttcga cctggtaggc cccgccatgg ttgggccgtc cagctcccat acagccggtg
      721 cgaaccgcat cggacagatt gcccggaatg tcattctggc cagggttgaa cggggcgaga
      781 cgattcagag cctcgaaatc aagctgatgg gctcattcag agataccgga ttgggacaca
      841 aaacgccatt ggcgcttgcc ggcggacttc aaggtctgac ggctgatgat gaaaccatgc
      901 tcagccatgg cgaaccggag tatctccgga caaacggttt tgtgttcggt gccggcaaga
      961 ccaggtttat cggttttacc cgcggcctcc cggaagacga ccttcgctat gccggcgaaa
     1021 aatgcaacaa tgttgcggaa atcattttca cgactgagtc cggcactcat ttcatcaccg
     1081 gcttttccat cggggccggc aacgtcgaga tcaggtatgt cgacacgttg ctcgagacgc
     1141 cgatcacggg aaagcgcgac ttctaccttg acggcgacac gattgtggca actcaaaacg
     1201 ccgcaggcac gttgccggtg attcgtggta ttttcgatcg caacgagccg gagaccggct
     1261 acgtcatgcg attcaacacg ctcgaagagc tgatcggtta ttgtcggcgg gaacgtaaaa
     1321 gactggtcga tgtcgtgatt gacgtggaat cgggcatgta tgcgatgacc cgcgaagaca
     1381 tcttccaaaa gatgcggatc tactggagag ccatggaaaa ttccgtgaaa accggcattg
     1441 ccggtcggaa aatgtcgatg ttgggtctct cgggtgactc gggattcaaa ctcaacggct
     1501 atgccgccgg gcatcccctg ttcgacaata tttacggaag agcggttgca tacgccaccg
     1561 cggtgaacga agtgaacgcc agaagcggcg tcatcgtcgc ctgccctacc gccggatcct
     1621 gcggaattct tccgggtgtg ttacgggcct actccgaatt ggcgaatcct cctgaggagc
     1681 ggattctgga atcgcttctg gtttcgggtt ttctgggaat gcttctcttc accgacgtgt
     1741 cgatggccgg agccgaccat ggctgtcagg ctgaggtcgg ctcggctgcc gcgatggccg
     1801 ccgccgccct ttgttatcag gagggcggag acgtcgagca ggtgatcgct gcttttacgc
     1861 tggcgatcaa gaacagtctc gggctgatct gcgacccgat tgcgggattg gtcgaagttc
     1921 cttgcgtcaa acgcaacggt gtctattcgt cactcgccat aagtgccgcg atgatggcgc
     1981 tgtccggcat aaaatcctac gtatctcctg at
//
LOCUS       DAORHD010001000         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71352_length_2011_cov_4.212679, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001000 DAORHD010000000
VERSION     DAORHD010001000.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71352_length_2011_cov_4.212679"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1237
                     /gene="buk"
                     /locus_tag="PKO06_17960"
     CDS             149..1237
                     /gene="buk"
                     /locus_tag="PKO06_17960"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNV71595.1"
                     /translation="MKGPFKILVVNPGSTSTKLALFEDEKLLAERKAQHPASEIEKFP
                     RILDQFPMRRQVMLDFLRDQGLEPGQLSAVVARGGLMKPVPSGTFHVTSKMVEDLTEA
                     RYGEHACNIAAILAQGFQWDYDIPAYVVDPVVVDEMNDVARLSGLKEIPRRSIWHALN
                     IMAVTRLLCSREHWNFRQESFVVAHLGGGITVAAIQRGRCIDVSNGLEEGPFTPERSG
                     ELPVLELIKMCFSGKYTHDEMKKMAVGRGGLVNYLGTNDLFEVEKRLAQGDDYAKLVV
                     RAMAYQVAKTIGSYHVVLRGKAKGIILTGGGARFPEFVAQIKEAVEYLCPVYLMPGED
                     EMSALAMGGLRVLRGEEKSLDYDSPPSN"
     gene            1280..>2011
                     /locus_tag="PKO06_17965"
     CDS             1280..>2011
                     /locus_tag="PKO06_17965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV71596.1"
                     /translation="MEFDPRDQELQKQWQKIATDVVAPVAATIDATGEIPESLLRHLD
                     ELKLLTPFLPTELGGEDRGLQSLSILLEEIARHSPVTALLCAQQVVVGIRANLRCFNL
                     GDRSEWGKKASQFKAIFTLAASENEAGSDLTSIQTTCRRTADGEFVLNGEKAYVNWAA
                     RAAWIIALARTQEQEGRGFSLFAVPGSAVGLEFDASHPTMGLQGIAAAPIRFRDVKLT
                     AERVAGVYGFGFDPYDQMLNEMRICV"
BASE COUNT          419 a          541 c          617 g          434 t
ORIGIN      
        1 ctcgaagagt cgaaaattcg gaagatcaac ccgtttgtcc tggctcgcca acacgcacaa
       61 gagcgtatgg cgcgcacgca gagcctgaga aaaatccatc gtccgtcgcg gcgataaccg
      121 caatcaagga aagaaactgg tgaatgacat gaaaggcccg ttcaagattc tggtcgtgaa
      181 ccctggttcg accagtacca aactcgcctt gttcgaagat gagaagttgc tggctgagcg
      241 caaggctcag catccggctt ctgagatcga aaaatttccc cgtatcctcg accagtttcc
      301 catgcgtcga caggtgatgc tcgactttct gcgcgatcag gggctcgaac cagggcagct
      361 gtcggcggtc gtggcccgtg ggggcctgat gaagcccgta cccagcggta cttttcacgt
      421 cacctcgaaa atggtcgaag atctgacgga agcccgttac ggtgaacacg cctgcaatat
      481 tgccgccatt ctggcccagg gcttccagtg ggattacgac attccggcct acgtggttga
      541 cccagttgtc gtcgatgaga tgaatgatgt ggcacgactc tcaggcctga aggaaattcc
      601 ccgtcgcagt atctggcacg cgctcaatat catggcggtc acgcgattgc tgtgttcccg
      661 tgaacactgg aacttccgtc aggagagttt cgtggtggcg cacctcggtg gcggcattac
      721 cgtggccgcg attcaacgcg gtcgctgcat cgatgtcagc aacgggctcg aggaaggccc
      781 cttcaccccg gagcgttccg gtgaactgcc ggtgctggaa ctgatcaaaa tgtgtttttc
      841 cggaaaatac acgcacgatg aaatgaagaa gatggccgtc ggccgcggtg ggctggtgaa
      901 ctatctcggg accaatgacc tgttcgaagt cgagaaacgc ctggcacagg gtgatgatta
      961 cgcgaaactc gtggtgcgag ccatggcgta tcaggtggcc aagaccatcg gttcctatca
     1021 cgtggtactt cgcggcaaag ccaaagggat catcctcacc ggcggcggcg cgcggtttcc
     1081 cgagttcgtg gcgcagatca aggaagctgt cgagtatctg tgtccggtct acctgatgcc
     1141 cggagaagac gaaatgtcgg ccctggcgat gggtggtctg cgcgtgctgc gcggcgaaga
     1201 aaagtctctc gattatgaca gcccgccctc gaactgaggt cgggttgagc ctcttcacga
     1261 aaggacctga gccgtgaaca tggaatttga tccccgggat caggagttac agaaacagtg
     1321 gcagaagatc gcgaccgatg tggttgcgcc tgtcgcagcc accattgatg ccaccggtga
     1381 gattccggag tcgttgctgc gacatcttga tgaactgaag ctcctgacgc cttttctgcc
     1441 cacagagctt ggtggtgaag atcgcggtct gcagagcctg agtattcttc tcgaggagat
     1501 cgctcgccat tctccggtca cggcactgct gtgcgcgcag caggtggtgg tgggcattcg
     1561 cgcgaatctg cgctgtttca acctcggtga ccgcagtgag tggggtaaga aagcgtccca
     1621 gttcaaggcc atctttacgc tggcagcatc agagaatgag gccggtagtg atctaacttc
     1681 catccagacc acctgccgca gaacggccga tggcgaattc gttctgaatg gagaaaaagc
     1741 ctatgtgaac tgggcggctc gtgccgcctg gatcattgct ctggctcgca cgcaggagca
     1801 ggaaggccgc ggtttttcgc tgttcgcggt tccggggtca gccgttgggc tcgagtttga
     1861 tgcgtcgcat ccgaccatgg ggctgcaggg cattgccgca gcgccgattc gctttcgtga
     1921 tgtgaagctg accgccgagc gtgtggctgg cgtctatggt ttcgggtttg acccctatga
     1981 tcagatgctc aatgagatgc gcatctgtgt c
//
LOCUS       DAORHD010001001         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71464_length_2009_cov_4.403787, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001001 DAORHD010000000
VERSION     DAORHD010001001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71464_length_2009_cov_4.403787"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PKO06_17970"
     CDS             <1..381
                     /locus_tag="PKO06_17970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71597.1"
                     /translation="EFPEQLPTLVGDPAQLKQVFLNILTNAIQASRPEGTLTVQILIE
                     SQAVRASIIDTGVGIPKDKIKAIFDPFMTTKEDGTGLGLPLALRIVEEHNGHIEVEST
                     EGSGSRFSILLPLPTDTSGVSGHD"
     gene            374..1060
                     /gene="coaE"
                     /locus_tag="PKO06_17975"
     CDS             374..1060
                     /gene="coaE"
                     /locus_tag="PKO06_17975"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNV71598.1"
                     /translation="MIDQSSFTDSPLNLTTGSSIVLGITGQIAAGKSTLAGILCEFGG
                     VNLEVDEVGHRMLANPEVIQKLTEAFGSDILDSSGSVSRKILGRRAFASPATNRQLND
                     IMHPPMILEVSRFVQVEAEKKTALIIVNAALLYTMGLDRLCDAIVYVRAPANQRLNRM
                     ITARGMPMEAAKARLFAQDPEPLQGPRVFFCDNQGPFLDLCQWVSEFFLPQLEGVFPC
                     HLLHQPPRQP"
     gene            1151..1969
                     /locus_tag="PKO06_17980"
     CDS             1151..1969
                     /locus_tag="PKO06_17980"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HNV71599.1"
                     /translation="MTAQNLRPHCPISNPELEKAILALPSIDEIPHCFLQNLHRAVFL
                     SPIANANGDACSDIRADSRYTFVPSSEGGKSYLALFTSVEQLGHWRSEQVHAHIHLSL
                     LEAVNAARNHKYEGLILNPGSTRHMRLSHQAFDKVLAISPRHQQQKSETTESELAIEQ
                     GDEMIVSTPDPAPPEVFLHELSRVLRTIDGLRDAFLFELAWKDTSGDLVLGLQLSTPP
                     RPGQIEAALDQISSEAREAAAGFDHFDLMLLDDPALIEVVSSTVPSLLASGPAN"
BASE COUNT          459 a          630 c          495 g          425 t
ORIGIN      
        1 gaattccctg aacaactgcc caccctggtc ggcgacccgg ctcaactcaa acaggtcttt
       61 ctcaacattc tgaccaatgc cattcaagcg tctcgtcctg aaggaactct gacggtgcag
      121 atcctcattg aaagtcaagc cgtgcgtgcc tcaatcattg acaccggagt aggcattccg
      181 aaagacaaga tcaaggcgat cttcgacccg ttcatgacca caaaagagga cggaaccgga
      241 ctcggcctgc cacttgccct ccgcatcgtc gaagaacaca acggtcacat cgaagtcgaa
      301 agcactgaag gaagcggttc acggttttcc atccttcttc cccttccgac cgatacttcc
      361 ggagtgagcg gacatgattg atcaaagcag cttcacggac agcccgttga acctcaccac
      421 cggttcatcg atcgtcctgg gaatcaccgg gcagattgcc gcgggtaaat ccacattggc
      481 cggtatcctc tgcgagtttg gcggcgtcaa tctcgaagtc gacgaggtcg gacaccgcat
      541 gctggcaaat cccgaggtca tacagaaact caccgaagcc ttcggcagcg atattcttga
      601 ttcatccggc tccgtcagtc gaaagattct cggaagaaga gcatttgcct ctccggcaac
      661 caatagacaa ctcaatgaca tcatgcaccc acccatgatc cttgaggtta gtcgatttgt
      721 ccaagttgag gcagaaaaga aaacagcact gatcatcgtc aacgcagctc ttctctacac
      781 catggggctc gaccgactct gcgacgccat tgtctatgtc cgcgcccccg cgaaccagag
      841 actgaaccgc atgatcaccg cgcgcggcat gcccatggaa gccgcgaaag cgcgtctgtt
      901 cgcccaggac cccgaaccgc tgcaaggtcc acgggtgttt ttctgcgaca accagggacc
      961 gtttctcgat ctctgtcagt gggtttctga gttttttctg ccgcagctcg agggcgtgtt
     1021 cccctgccac ctgctgcatc agcctccccg gcagccttga gaatctggta tcatggagtc
     1081 gcgtgcgtca ttgttctctg ctgctggggt gatcgaatca ccccgtaccg cctgacgagg
     1141 agtgcgaccc atgactgccc agaacctgcg acctcactgt cccatctcca accctgaact
     1201 ggagaaagct attctcgctc tcccgagcat tgatgagatt ccccactgtt ttctgcagaa
     1261 tctgcatcga gcagtttttc tcagcccgat cgccaatgcc aatggagacg cctgctcaga
     1321 cattcgtgct gattcgcggt acacctttgt gcccagttcc gaaggcggaa aatcgtatct
     1381 agcactgttc acatctgtcg aacaactagg acactggcgg tctgagcagg tccacgcgca
     1441 tatccatctt tcgcttctcg aagccgtcaa tgcagcacgc aatcacaagt atgaagggtt
     1501 gattctcaat cccgggtcaa ccagacatat gcgactgtca caccaagcgt ttgacaaagt
     1561 actggctatc tctccgcgtc atcagcagca gaagagcgaa acaaccgaaa gcgaactggc
     1621 catcgaacag ggtgacgaaa tgatcgtctc gacacccgac ccggctccgc ccgaagtctt
     1681 tcttcacgaa ctgtcacgtg tattgcgaac catcgacggc ctgcgcgatg cctttctgtt
     1741 cgaactcgcc tggaaagata cctcaggcga tctggtactg ggtctgcaac taagcacacc
     1801 accgcgacca gggcagatcg aggcggccct cgaccagatc tcgtccgagg cccgcgaagc
     1861 cgctgccggg ttcgatcact tcgacctcat gctgctcgac gacccggccc tcatcgaggt
     1921 tgtcagctcg actgttccct cgttgctggc cagtgggcca gccaactgac catgggtcat
     1981 cacgagcacg aggcggaagc tcctcaatc
//
LOCUS       DAORHD010001002         1990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71478_length_2009_cov_3.528147, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001002 DAORHD010000000
VERSION     DAORHD010001002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71478_length_2009_cov_3.528147"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKO06_17985"
     CDS             complement(<1..692)
                     /locus_tag="PKO06_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNV71600.1"
                     /translation="MKRLAILFALCLLVVPVVADVLYLNEGEEHVGRLLEIAGGKIRF
                     QEPGGAEKAFNASEVTHVLLSQIREGDEISQVASLTEPMLVQLLASAPQATDFPDSDF
                     ITLYRLRDFTFHPDGRVSFMRRRIVKVLKEPGLDEANHSLYYFRDREKLDLDFAHTYN
                     PDGRIYHLTDDALSEETLYPNLPEYDFLNKVKFAMKKVDLGSVVDIKHRVETRDVGPL
                     NPYIIDTVFGER"
     gene            complement(689..1918)
                     /locus_tag="PKO06_17990"
     CDS             complement(689..1918)
                     /locus_tag="PKO06_17990"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HNV71601.1"
                     /translation="MPPMRDVLPIVVFSKQKDWKYMNDRMRPMFEKRFQLTDVVKKKV
                     DEIIAGAKTIEDKIARLYLFCQKEIRYISIKGNLASNQVGHPAEETLKNRYGDCTDKG
                     MLLATMLKHIGVEAYPVGVLTNDAGRAFRELPIFDSNHCITEVNLNGRRFYLDSTATD
                     YRYPYFRSDDHDTIAENNMRGSIDPIPVPPPEDNAIVGRRQLKIERDGTMRIDLENTQ
                     NGSWEASMRGMVRNFKPEEYEKQVRASISGLTASYTLHVATFSNPLDFSGPFTTRSGY
                     TLHQYVPRSGRYLVLEIPFFGLRFPEVALAERTYDIVYTTSNLRDDQIEIQLPPGAKV
                     KYLPPNVDVKTPALEYSCVYKVEGEKILVQRRLAVKQRVVPVATYTTYKAELEKIARS
                     TDERIFLEDTEGKEESR"
BASE COUNT          429 a          555 c          545 g          461 t
ORIGIN      
        1 tctcgttcac caaaaaccgt atcgatgatg tacgggttga gcggaccgac atcgcgcgtc
       61 tcgacccggt gtttgatgtc cacgaccgag ccgagatcca ctttcttcat ggcaaatttg
      121 actttgttga ggaagtcata ctcgggcaga ttaggataca gcgtctcttc cgagagggcg
      181 tcatcggtaa gatgatagat gcgcccgtcg gggttgtagg tatgcgcgaa gtccagatca
      241 agcttttcac gatcgcggaa gtagtacagc gaatggttgg cttcgtccag accaggttct
      301 ttgagcactt tcacgatgcg ccggcgcatg aagctgacac ggccatccgg gtgaaaggtg
      361 aaatcacgca gccgatacag cgtgatgaaa tccgagtccg gaaagtccgt ggcctgcggc
      421 gcggatgcca gtaattgtac cagcatcggc tcggtcagac tcgcaacctg actgatttca
      481 tcaccttctc gtatttgaga cagcagcaca tgggtgactt ccgaggcatt aaaagccttt
      541 tccgcccccc cgggttcctg gaaccggatc ttccctccgg cgatctccag caggcgaccg
      601 acgtgttctt ccccttcatt cagatacaga acgtcggcaa cgaccggcac aaccagcagg
      661 cagagagcaa acaggatggc gaggcgtttc atcgggactc ctcctttcct tccgtgtctt
      721 ccaggaagat gcgttcatcg gtcgaacggg caattttctc gagttcggcc ttgtacgtgg
      781 tataggtcgc caccggcaca acacgctgtt tcaccgccaa tcggcgctga accaggatct
      841 tctctccctc taccttgtac acacacgaat actcgagcgc gggagtcttg acatcgacgt
      901 tcggtggcag atacttcacc ttcgccccgg gcggcagttg aatctcgatc tgatcatcgc
      961 gcagattcga ggttgtatag acgatgtcat aggttctctc cgccagcgcc acttccggaa
     1021 aacgcagacc gaagaacgga atctcgagta ccagataccg gccggagcgc ggcacatatt
     1081 gatgcagcgt gtatcccgat cgggtggtga aagggccact gaaatcgagc gggttcgaga
     1141 aggtcgccac atgcagcgta tacgaggcgg tgagccccga aatcgaagca cgcacctgtt
     1201 tctcgtattc ctccggtttg aagttgcgca ccatgccgcg catagaggct tcccaggagc
     1261 cgttctgcgt attctcgaga tcgatacgca tcgtcccatc gcgctcgatc ttcaactgtc
     1321 gacggcccac aatggcattg tcttcgggcg gcggtacggg gatgggatcg atcgagccac
     1381 gcatgttatt ctcggcgatc gtatcgtggt catctgaacg aaagtaagga taccgatagt
     1441 cggtggcggt ggagtcaaga tagaaccgcc gaccgttcag gttcacttcg gtaatgcagt
     1501 ggtttgaatc aaagatgggc agttcgcgaa aggcccgccc ggcatcattg gtcagcacac
     1561 cgacgggata agcttcgacc ccgatgtgtt tcagcatggt cgccagcagc atgcctttgt
     1621 cggtgcaatc gccataccgg tttttcagcg tttcctcagc cggatgcccc acctgattgc
     1681 ttgccaggtt gcctttaatg gaaatatagc ggatctcctt ctgacaaaac aggtacaaac
     1741 gcgcgatctt atcctcgatc gttttcgcgc cggcgatgat ttcatcgacc tttttcttca
     1801 caacgtcggt cagctgaaaa cgtttttcga acatggggcg catgcgatcg ttcatgtact
     1861 tccagtcttt ctgtttgctg aacacgacga tgggcagcac atcccgcatg ggcggcatat
     1921 aggcttccga gatgatcggc ggcacattct cggaagccta tatgccgccc atgcgggatg
     1981 tgctgcccat
//
LOCUS       DAORHD010001003         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71702_length_2005_cov_3.215897, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001003 DAORHD010000000
VERSION     DAORHD010001003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71702_length_2005_cov_3.215897"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PKO06_17995"
     CDS             <1..1141
                     /locus_tag="PKO06_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71602.1"
                     /translation="VIDFERAPDAGMAPTRIGLSAQYQGTIGQMFLLPGRSYPSGQPA
                     FSVKRAAQPRRAPQTLKRLVSLRRHTADTVESYNLAHRLDRYAIHQMNSAEATATVHG
                     AEVWLSREAELVTALQGLPERLTPERLRRELAGFVPQGPTSTQAMVNRQLLKRLGNFI
                     PPSLAESSAPLSCFVFPEAPWRTLRPDIAESLELLSAMQDRDCLIEAETLLSTVATAP
                     IFHGLPTFLAQRRGLSGASFNVDQDHDSFCRTLAEAPGTAIRVSLPLNPAGGVPPSAI
                     ANQLVNSLDHFYFSGHDRYYQIPRPEETAIASVSMPYWLRYHARVTPGAGTSAPFLMR
                     VSDLLSFFMKESVDPAPEIPARETRITAPPSNGPATGSCITGIMR"
     gene            complement(1330..>2005)
                     /locus_tag="PKO06_18000"
     CDS             complement(1330..>2005)
                     /locus_tag="PKO06_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71603.1"
                     /translation="KMRVRSDVRRCEDLYVEMSREPKVLQRAVEKLAEVSTRDLDILD
                     KLIESRSKAYPRPLMVPKQVVSEFTMLRALRRVAVNNALHLILTAKSPAFQQMAGTPD
                     KDTMKKLEKLAAVVGASPVFPRGAAPEAGEPAVAGGQTLTTADQTSTKNLGQLIIPEG
                     MTLEKAQKEYQAALQAYTQIAQSGDESKSREAYDVYQVKYAVYQAMLEKFNEEEQKKE
                     EALTGR"
BASE COUNT          380 a          667 c          527 g          431 t
ORIGIN      
        1 tgtcattgac tttgaacgag ctcccgacgc aggtatggca ccaacgcgaa taggactttc
       61 ggcacagtat caaggcacca tcggtcagat gttcctgtta ccggggcgat cgtatccgag
      121 cgggcaaccg gccttttccg tcaaacgagc ggcacaaccc agacgggcac ctcagacgtt
      181 gaaacgcctt gtcagcctgc gccgccacac cgcagacact gttgaatcct acaatcttgc
      241 ccaccggctc gatcgctatg ccattcatca gatgaactcc gccgaagcaa cggcgaccgt
      301 tcatggcgcg gaggtgtggc tctcgcgcga agccgagctc gtcacggcgc tgcaaggtct
      361 tcccgagcgc ctgactccgg agcgtcttcg cagagaacta gccggtttcg ttccccaggg
      421 tcccacatcc acccaggcga tggtcaatcg gcagctgctc aagcgcctgg gcaacttcat
      481 tcccccgagc ctggcagagt catctgcgcc cctgtcctgt ttcgtgtttc ccgaagcgcc
      541 gtggcgaact cttcgcccgg atatcgccga gtctctcgag ttgctcagcg ccatgcagga
      601 tcgtgattgc ctgatcgagg cggaaaccct gctgagcacg gtggcaaccg ctccgatctt
      661 tcacggtctg ccaacgtttc ttgctcagcg ccgtggactt tccggcgctt ctttcaacgt
      721 tgatcaggat cacgattcct tctgccggac acttgcagag gcgcctggaa cggcaattcg
      781 cgtatcgctc cctctcaacc cggctggtgg ggttcctccg tctgcgatcg ccaatcaact
      841 cgtcaatagc ctggatcact tctatttctc cgggcacgac cggtactatc agattccccg
      901 acctgaggaa acagcgattg ccagcgtctc tatgccgtat tggcttcgct accacgcaag
      961 agtcacaccg ggagcaggga ccagcgcacc gtttctcatg cgtgtctcag acctgctctc
     1021 cttcttcatg aaagagtccg ttgacccggc tcccgagatc ccggctcgcg aaacaagaat
     1081 caccgccccc ccttcgaacg gcccggccac gggttcatgc atcaccggca tcatgcgctg
     1141 acctgaagca gccgacagag agctgttcat gcgcccgcca tgccagtgcg gttcgaacgc
     1201 acgtcaccgg tgcagattcc ccggcacctt cgctacgaac ggctccgctt ggcgaagaac
     1261 aagaccgcgg aggccggaaa gtccgacccc cgcggtttga cgacaaacgc ttgcgaaagc
     1321 gacgctgact cagcgtcccg tcagcgcttc ttccttcttc tgttcttctt cattgaactt
     1381 ctcgagcatg gcctgataca ccgcgtattt cacctggtag acatcatacg cttcgcggct
     1441 cttgctttcg tcgcccgatt gcgcaatctg cgtgtaggcc tgcagggccg cctggtattc
     1501 cttctgcgct ttctccaggg tcatcccctc aggaatgatc agttgcccca gattcttggt
     1561 cgaagtctga tcggcggtcg tcagggtctg acctccggca acagcaggct ctccggcttc
     1621 aggtgcggct ccacgaggga acacgggtga agccccaaca accgcagcca gcttctcgag
     1681 cttcttcatc gtgtccttgt ccggagtgcc cgccatctgc tgaaacgccg gggacttcgc
     1741 ggtcaggatc aggtgcaacg cattattcac cgcgacacgg cgcaacgccc gcaacatggt
     1801 gaactcgctg acgacctgct tcggtaccat caacgggcga ggataggctt ttgaacgtga
     1861 ctcgatcagt ttgtccagaa tatcgagatc tcgggtcgag acttcagcca gcttctcaac
     1921 cgcacgttgc agcactttcg gctctcggct catctcgacg tacaagtctt cacaccggcg
     1981 cacatcgctg cgaacgcgca tcttc
//
LOCUS       DAORHD010001004         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71713_length_2005_cov_2.569744, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001004 DAORHD010000000
VERSION     DAORHD010001004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71713_length_2005_cov_2.569744"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PKO06_18005"
     CDS             <1..858
                     /locus_tag="PKO06_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71604.1"
                     /translation="YSGKPVLANVSFTVPKGQVVALLGRNGVGKTTLMRLMNGLTAAS
                     QGRIEILGHDPATDVVELRQRLGYLGEECHIYPWMTGEDARYFMAPLYRTWDNAYFHE
                     LVKQLQVPLNQKLQDLSKGSRRKLHLALTLAHRPEVILLDEPLSGLDPVVRDQVISTL
                     IHTLTEEGRTILLSTHEIADIETICDRVLLLKEQRLALDMERDAIAASVRRVTIRLEN
                     PAAIVPKHPDILAVTARGCEMELIVRKYSDTALSEMLANLKVVDRQVSGTSLKQLFIT
                     LTEQQEEVV"
     gene            855..>2005
                     /locus_tag="PKO06_18010"
     CDS             855..>2005
                     /locus_tag="PKO06_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71605.1"
                     /translation="MNILFWKQYIELRSSLLVMVLFGIAMVPILSYFNYYETSTLVFV
                     LFCLVGPILSLQIGIMVFRPEFVENTIQFLDQLPVERHRVWLVKVVSGLIMVLVLYVS
                     YLIISYFGFNDHVSLNNPLGNGLRVPVPLLVLTPLFFFSYGVINALLPVWASTILLLL
                     LVWLAFIVLASHLLFFVNWLLAIPALVVFMLTISRYAFIRGEVDDATARLWRVCKAGL
                     MGLVVLFLLTICLDQVAQARIGSRMTSANSRPIATMADNSLIVSFNCESALYDPISSS
                     HMVQYVRIEPTTGEKTLIGPRLSSDLVLGQEGKVAAFSTAYHWPGIVGLGFTATNRNI
                     IPYNAMMLYDPAYSSVPVKIDTFAVPIGFSKAQSLWYYRYQNSVTELVEA"
BASE COUNT          384 a          560 c          551 g          510 t
ORIGIN      
        1 tattccggca aaccggtcct ggcaaacgta tccttcaccg tgcccaaagg ccaggtcgtg
       61 gctctgctcg gacgcaacgg cgtcggcaag accacgttga tgcgtctgat gaacggcctg
      121 accgccgcct cacagggccg catcgaaatt ctgggtcacg accccgcaac cgatgtggtc
      181 gaactgcgtc agcgccttgg ctatctcggc gaggaatgtc acatctatcc gtggatgacc
      241 ggtgaagacg cgcgctattt catggctccg ctgtatcgca cctgggacaa cgcctatttc
      301 catgagttgg tgaaacagtt gcaggtgccg ctgaaccaga aactgcagga tctcagcaag
      361 ggctcgcgcc gcaaactgca tctcgcgctc accctggcgc atcggcccga agtgattctg
      421 ctcgatgagc cgctttccgg gctggacccg gtggtgcgtg atcaggtgat ctcgacgctg
      481 attcatacgt tgaccgagga gggccggacg attctgctct cgacccatga gatcgccgac
      541 atcgagacca tctgtgatcg ggttctcttg ctgaaagaac agcgtctcgc tctggacatg
      601 gagcgcgacg cgatcgctgc ttccgtgcgc cgggtgacga ttcgactcga gaaccccgcc
      661 gcgatcgtgc cgaaacatcc ggatattctg gcggtaaccg ccagagggtg cgagatggag
      721 ttgatcgtcc gcaagtattc ggataccgct ttgtccgaga tgctcgcaaa tctgaaggtc
      781 gttgatcgcc aggtttcggg cacctcgttg aaacaactat tcattactct cacggaacaa
      841 caggaggaag tcgtatgaac atccttttct ggaaacaata catcgagttg cgttcttctc
      901 tgctggtcat ggttctgttc ggtattgcca tggtgccgat tctgagctat ttcaactact
      961 acgaaaccag tacactcgtc ttcgtgttgt tctgcctggt cgggccgatt ctgtcactgc
     1021 agatcggaat catggtgttc agacccgagt ttgttgagaa cacgattcaa tttcttgacc
     1081 agttgcccgt agaacggcac cgggtctggc tggtcaaggt cgtcagcggg ctgatcatgg
     1141 tgctcgtcct gtatgtgtcc tatctgatca tcagttactt cggcttcaat gaccatgtct
     1201 cactgaataa cccccttggc aacggacttc gggttcccgt acccctgttg gttctcacgc
     1261 cgctcttctt cttcagctat ggagtcatca acgcgcttct gccggtctgg gcgtcgacga
     1321 ttcttttgct cctgttggtc tggctcgcgt tcatcgtcct tgcttcccac ttgttattct
     1381 ttgtgaactg gttgttggcc attccggcgt tggtcgtctt catgctgacc atctcgcggt
     1441 atgcgttcat tcgcggcgaa gtggatgacg cgacggcccg actgtggcgg gtttgtaaag
     1501 ccggcctgat gggactcgtc gtgctctttc tgctgacgat ctgtctggat caagttgctc
     1561 aggcacggat cggctcgagg atgacttccg cgaactctcg accgattgcc acgatggctg
     1621 ataacagtct gattgtcagc tttaactgcg aatctgcctt gtacgatccg atcagttcct
     1681 cgcatatggt gcagtatgtt cgcattgagc cgacaaccgg cgaaaagacg ctgatcggcc
     1741 cgcggttgtc gagtgatctg gtgctcggtc aggagggaaa ggtcgctgcg ttctcgacgg
     1801 cataccactg gcctggtatc gtcggtctgg gctttaccgc gacaaaccgc aatatcattc
     1861 cttacaatgc gatgatgctc tacgatccgg catattcttc cgtaccggtc aaaatcgata
     1921 cattcgcagt tccgatcgga ttctcaaagg cgcagagtct ctggtactat cgctaccaaa
     1981 actcggtgac cgagctggtt gaagc
//
LOCUS       DAORHD010001005         2001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_71926_length_2001_cov_3.511305, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001005 DAORHD010000000
VERSION     DAORHD010001005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71926_length_2001_cov_3.511305"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKO06_18015"
     CDS             complement(<1..863)
                     /locus_tag="PKO06_18015"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71606.1"
                     /translation="MNLSLIFERRPQASYSFTILVLFCSLATGCIAAALLFLLRGVSP
                     WLALQKIFVGSFGSWYGFQETVAKAIPLALTGIGLTVAFRARFWNIGAEGQILAGAVG
                     ATAVALYGPQGGSGVVTITLLFLAGAFCGALWAFIPALLRLRWQVNEVISTLMLNYIA
                     IELVQYLIYGPWKGKSQMGFPYSDDFPSAATLPTWGYTRIHLPTLILTLLAAAALWIF
                     LQRLTYGYEIRVSGENPDAAAYAGIPSGRVLVLAMMISGALAGCAGVGEVAGIHRHLT
                     YPWAISSGYGYT"
     gene            complement(860..>2001)
                     /locus_tag="PKO06_18020"
     CDS             complement(860..>2001)
                     /locus_tag="PKO06_18020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71607.1"
                     /translation="QRVEILKALISGCRILFLDEPTSVLTPPETRHLLEVLRRFAASG
                     GAVIFITHKLEEVLSVASRLTVLRHGRVTGTLAASGTTPADLARLMIGRALSFDRIPR
                     SRPIPENILLEVANLSVQDDRGRLRVSELSFNLREGEILGIAGVSGNGQRELVEALTG
                     MRRPISGTARYHEADLVQLDARSIIDAGVAHIPEDRLRHGVAGTLSLLENAVLSSFHR
                     PPFSRASFLQSPQIMESTNRIIDEFDVAVPQLESRRQTASTTAHSGRNLQASAKNLSG
                     GNLQKFIVGRELGRQPSLIIASHPTYGVDVGAAETIHQRLLGCRDAGSSVLLVSEDLT
                     ELFRLSDRIAVMYGGRFAGIVPPDPTNLDLIGQLMTGSRPPESHP"
BASE COUNT          439 a          559 c          663 g          340 t
ORIGIN      
        1 gtgtagccat accctgaaga gatggcccac ggatacgtga gatgccgatg gattcccgcg
       61 acttcgccca caccggcgca cccggccagt gctccgctga tcatcatggc cagcaccagc
      121 acgcgcccgc tggggatgcc ggcgtaggcc gctgcatccg ggttttcccc actgacgcga
      181 atctcgtagc cgtaggtcag tcgctgcaga aagatccaca gtgcggcggc tgccagcagg
      241 gtgagaatca gtgtgggcag atgaatgcgg gtatagcccc aggtcggcag ggtggccgcg
      301 gacggaaagt cgtcggaata gggaaacccc atctgtgatt tccctttcca gggaccgtag
      361 atgagatatt gtaccagctc gatggcgatg tagttcagca tcagggtcga aatcacctcg
      421 ttcacctgcc agcgcaggcg aagcagagcg gggatgaatg cccagagcgc tccgcagaag
      481 gctccggcca ggaacagcag cgtgatcgtg acgacccctg aaccgccctg cgggccatac
      541 agcgcgacag ccgtcgcacc gacggcaccg gccaggatct gcccctcggc gccgatgttc
      601 cagaaacggg cacgaaaggc cacggtcaga ccgatgccgg tcagcgccag cggaatggct
      661 ttcgcgacgg tctcttgaaa gccgtaccag ctgccgaaag agccgacaaa gatcttctgc
      721 agggcaagcc agggcgagac cccccgaagc agaaagagca gtgcggcggc gatgcagccg
      781 gtcgcgagag aacagaagag tacgagtatg gtgaacgaat acgaggcctg aggtcttcgt
      841 tcgaagatga gagaaagatt catgggtggg attccggagg gcggctgccg gtcatcaact
      901 gaccgatgag atcaagattc gtcgggtcgg gtggcacgat gccggcaaat cgaccgccat
      961 acatgacggc gatgcggtca gacaagcgga acagttcggt cagatcttcg gacaccagca
     1021 gaacggagct cccggcgtcg cggcacccaa gcaggcgctg gtggatggtc tcggcggcac
     1081 cgacgtcaac cccatacgtc gggtgcgaag cgataatcag actgggttgg cggcccaatt
     1141 cacggccgac gatgaacttc tgcagattgc cgcccgagag atttttggcc gaagcctgca
     1201 ggtttcggcc agaatgagcg gttgtcgatg cagtctgtcg gcggctctcg agctgtggaa
     1261 ccgccacatc gaactcatcg atgatgcggt tggtggactc catgatctgt ggtgactgca
     1321 gaaacgaggc tcgtgaaaac ggtgggcgat ggaacgatga caggaccgcg ttttcgagca
     1381 gtgatagagt tccggcgacc ccatggcgca gacggtcttc cggaatgtga gccactccgg
     1441 catcgatgat ggagcgggca tcgagttgca ccagatcagc ctcgtgatat cgggctgtgc
     1501 cgctgatggg ccgtcgcatg ccggtcagag cttcgacgag ttcgcgctgt ccgttgcctg
     1561 agaccccggc gatgccgagt atttctcctt cacgcaggtt gaaagagagc tcgctgaccc
     1621 gaagtcgacc acgatcatcc tggacagaca gattggcgac ttccagcaga atgttttcgg
     1681 ggatcggcct ggatcgcgga atgcggtcga atgacagagc tcggccgatc atcaatcgcg
     1741 ccagatcggc gggagtggtg ccagaagccg caagagtgcc ggtgacgcgg ccgtgtcgca
     1801 gcacggtgag acgtgaggca accgaaagaa cttcttcaag tttgtgggta atgaaaatca
     1861 ccgcgccgcc agaggcggcg aatcgccgca gaacttcaag caggtggcgg gtttcggggg
     1921 gggtgagcac cgaggtgggc tcatcgagaa agaggattcg gcaacccgag atcagggcct
     1981 tgaggatctc cacgcgctgc t
//
LOCUS       DAORHD010001006         1998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72113_length_1998_cov_3.538343, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001006 DAORHD010000000
VERSION     DAORHD010001006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72113_length_1998_cov_3.538343"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..>1998)
                     /locus_tag="PKO06_18025"
     CDS             complement(205..>1998)
                     /locus_tag="PKO06_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71608.1"
                     /translation="DLKNPVNLGQAEASGQMVELLTRVQALPEAGADAAIDKVIAELF
                     VMMDKKLRLTAMGAADQEKARIKAVLLKVRGMSPIDFELQNADLAKEAKPRFVDQLHH
                     EAALFQEKMLKVIGGVSKIGKQLLHPQVIPMLRARLKAPAAVCDLTPADLKTRTELLA
                     TLNREGKPAPAVDKVAAFLGLNEQTKATFVEAIVDFQKRAFKILMTQNDRGVSPFLTM
                     MRAKASGLPPQESAALLHQAFVETFPGKTHSYFEELINEKLKTYSDIRRIMSVREFAR
                     LSELDVDLMDVRTGYEMGRGSMGEGEGGENAGELGEEPNLVAIVRHLRLDRAQGEKIH
                     AILTEHQKSIGLLFRQGEPGKTPFDALVKVFQSQGDKECVAAFLTTLQNRVASGTVTY
                     FEIMLDRNRETWRRLEKALPDDAYAALAHLGLDLMKVKTGYNPFTGDGCSWEDLEYQE
                     KGERTRKKHGIEKKATVTALFTLMSVQDETVKDKVVKALQEGQKKAFALMSQPGENGN
                     APIDQLKLTGGAEGMKVFFQALMGKVKGKETTFMKELETIKRDTLVKLKDILPNAPYQ
                     LFRSLNLDLLDIDAGYNIGFELMKRRVSSWW"
BASE COUNT          380 a          622 c          508 g          488 t
ORIGIN      
        1 ccccattgag caggaggttc acgttcaaca tctgactgtt cttcgccttc tcccctgccc
       61 aggaaacaga ccacagaccg acgatgcaca gcagcgccat cgccatgatg agcttgcttc
      121 ttcgtttcat tctccactcc tctcgatttc gctcggccga tgccggcaac ccttctctcc
      181 atcacacgcc cacggaaccg tcactcacca ccagctcgag acgcggcgct tcatgagttc
      241 aaaaccaatg ttgtaaccgg cgtcgatgtc gagcaggtcg aggttaagcg agcgaaacaa
      301 ctggtacgga gcattcggga gaatgtcctt cagcttgacg agtgtgtcgc gtttgatggt
      361 ttcgagttct ttcatgaacg tcgtctcttt gcccttcacc ttgcccatca acgcctgaaa
      421 gaagactttc attccctcgg caccgcctgt cagtttgagt tggtcgatcg gcgcattccc
      481 gttttcaccc ggttgtgaca tcagggcgaa tgcttttttc tgaccttcct gcagagcctt
      541 cacgactttg tctttgaccg tttcgtcctg gacactcatc agggtaaaca acgcggtgac
      601 ggtcgctttc ttttcgatac cgtgcttctt tcgcgtccgc tcccctttct cctgatactc
      661 caggtcttcc caggaacacc catcgccggt aaagggatta tacccggtct tgaccttcat
      721 gagatcgagg ccgagatgcg cgagagccgc ataggcatcg tcgggcagcg ctttctcgag
      781 tcgccgccag gtctcgcgat ttcggtcgag catgatctcg aaatacgtga cagtgccgct
      841 ggcgacccgg ttctgcaacg tcgtcagaaa tgcggccaca cattccttgt caccttgact
      901 ctggaatacc tttaccagcg catcgaatgg agttttcccg ggctcgccct gccggaacag
      961 gaggccgatg ctcttctgat gctcggtgag aatcgcatga atcttctcgc cctgtgcgcg
     1021 gtcgagtcga agatgccgga caatggcgac cagattgggc tcttcgccaa gctcgcccgc
     1081 gttctctccg ccctcccctt cccccatcga tccgcgcccc atttcgtagc cggtgcgcac
     1141 atccatgaga tccacatcca gctcgctgag gcgggcgaat tcacgcacgc tcatgatgcg
     1201 acgaatgtcc gagtacgttt tcagtttctc attgatcagt tcttcgaaat acgagtgcgt
     1261 ttttccagga aaagtttcga caaaagcctg atgcagaagc gcggcgcttt cctgcggcgg
     1321 caggccactg gccttcgcac gcatcatggt cagaaacggc gagacgcccc gatcgttctg
     1381 cgtcatgaga attttgaacg cccgcttctg aaagtcgacg atcgcctcga caaacgtcgc
     1441 tttcgtctgt tcgttgagtc cgaggaatgc cgcgacctta tcgactgccg gggcgggttt
     1501 tccttcgcga ttcaatgtcg caagcagttc ggttctggtc ttcagatcag ccggagtcag
     1561 atcgcagact gctgccggag ctttcaggcg agcccgcagc atcgggatca cctgcggatg
     1621 caggagctgc ttgccgattt tcgatacgcc cccgatcacc ttcagcatct tctcctggaa
     1681 cagagcagct tcgtggtgca gctgatcgac aaaccggggt ttcgcttctt tggccagatc
     1741 ggcattctgc aattcgaagt caatcggcga catgccgcgc accttcaata aaaccgcctt
     1801 gatacgggct ttttcctgat cggcggcccc catggcggtc aggcgcagct ttttgtccat
     1861 catcacgaac agctcggcaa tcaccttgtc gatggcagca tcagccccgg cctcgggtaa
     1921 tgcctgcacg cgtgtcagca gttcgaccat ctggccactc gcctcagctt gtccgagatt
     1981 caccggattc ttgagatc
//
LOCUS       DAORHD010001007         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72244_length_1996_cov_3.194745, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001007 DAORHD010000000
VERSION     DAORHD010001007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72244_length_1996_cov_3.194745"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PKO06_18030"
     CDS             complement(<1..540)
                     /locus_tag="PKO06_18030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71609.1"
                     /translation="MRQLYRFLLIVCLIISVIGAPNLLAAREMTVYTSPNGGFSPMNN
                     QRTVTFPDGTIASASLTNQALSMIRNVPNGGEIKIAMYSFSVEEVQFELIKAAGERDI
                     RVKVLLDNSAEWTQDTVELFIHRVKMAAEKYKNFDYQIKVTTKNSFVKTKRTRKLSDG
                     TMIYGTMHEKFLIFYDKKDG"
     gene            complement(621..1829)
                     /locus_tag="PKO06_18035"
     CDS             complement(621..1829)
                     /locus_tag="PKO06_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005037293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV71610.1"
                     /translation="MAKVQGNVLIAQGGGPTAVINQSLVGAVLEARKFPEVDRVYGAL
                     HGVRGIVDENFIDLTQATTHNLEKVACTPSSALLSTRDKPDEEYCKKIFQICMKHNVR
                     YFFYNGGNDSADAVRIVNNNAKAAGYELRCIHIPKTIDNDLRVTDHCPGYGSAALFVT
                     LAFAGANLDNRSLPGVYIGVVMGRHAGYLTAGSVFARKYDDDGPHLIYLPERTFKKEQ
                     FLKDVDAVYKKHGRCIIAVSEGIVGEDGKPVITQLTGNQERDAHGNVQLSGTGALGDL
                     LAETIKKDLKIKRVRADTLGYLQRSFVGIVSPVDQAEAREVGEKAAQFALWDNVDGSV
                     AIRRTGDYSVEYFLTPLETVAKETKHMPDSFISAAGNDVTTEFKDYARPLLGPLPEWE
                     RLMAPRVPKK"
BASE COUNT          390 a          547 c          603 g          456 t
ORIGIN      
        1 gccgtctttc ttgtcgtaga agatgaggaa cttttcgtgc atcgtgccat agatcatggt
       61 tccatcgctc aatttgcggg tacgtttggt tttgacaaag gaattcttcg tcgtgacctt
      121 gatctggtaa tcgaagttct tgtacttttc cgccgccatc ttcacgcggt gaatgaacag
      181 ttcgaccgta tcctgcgtcc attcggccga gttgtcgagc agcaccttga cgcgaatgtc
      241 gcgttcgcct gcggccttga tcaattcgaa ctgcacttcc tcgacactga agctgtacat
      301 ggcaatcttg atctcgccgc cgttgggaac attgcggatc atgctcagcg cttggttggt
      361 cagactggcc gacgcgatcg tgccatctgg aaaggtcacc gttcgctgat tgttcatcgg
      421 tgaaaaaccg ccattgggcg atgtgtacac cgtcatctcg cgtgccgcga gcagattggg
      481 ggcaccaata acgctgataa taaggcagac gatcaagagg aaccggtaca actgtctcat
      541 gcgatactcc ttacagcaaa ataagaaaaa agcccgaagg cgcgttcgcc ttcaggcctg
      601 gaaaaccaac atgaaggacg tcacttcttc ggaacacgcg gtgccatgag gcgttcccac
      661 tcgggcagag gccccagcag cggtcgggcg tagtccttga attcggtcgt gacgtcgttg
      721 ccagccgcgg aaatgaagct gtcgggcata tgcttggttt ctttggcgac cgtctcgagc
      781 ggggtcagga aatattcgac ggaataatcg ccggtgcggc gaatcgcgac ggagccgtcg
      841 acgttgtccc acagagcgaa ctgcgcggct ttctcgccga cttcgcgggc ttccgcctgg
      901 tcgaccggtg agacgatccc gacgaaactg cgctgcaggt agccgagggt gtcggcgcgt
      961 acgcgcttga tcttcaggtc tttcttgatg gtctcggcga gcaggtcgcc cagtgcgccg
     1021 gtgccggaaa gctgcacatt gccgtgcgcg tcgcgttcct gattgccggt cagctgagtg
     1081 atcacgggct tgccgtcttc tccgacgatg ccctcggaca ccgcgatgat gcagcgtccg
     1141 tgcttcttgt agacggcgtc gacgtctttc aggaactgtt ccttcttgaa cgtgcgttca
     1201 ggcaggtaga tcaggtgcgg gccgtcgtca tcatacttgc gggcaaagac gctgcctgcc
     1261 gtgagatagc cggcatggcg acccatgacg acgccgatgt agacgccggg cagcgagcga
     1321 ttgtccaggt tggcaccggc gaacgcgagc gtcacgaaca acgcggctga gccgtaaccg
     1381 gggcagtggt ccgtgacacg cagatcgttg tcgatggtct tgggaatgtg aatgcagcgc
     1441 agttcgtagc cggccgcctt ggcgttattg ttcacgatgc gcacggcatc ggccgagtca
     1501 tttccgccgt tatagaagaa ataccgcaca ttgtgcttca tgcagatctg aaagatcttc
     1561 ttgcagtatt cctcgtccgg cttgtcgcgt gtggaaagca gggccgagct gggagtacaa
     1621 gcgacctttt ccaggttgtg cgtcgtagcc tgggtgagat cgatgaagtt ttcgtcgacg
     1681 atgccgcgaa ccccgtgcag cgcgccgtac acgcgatcga cctcggggaa cttgcgggct
     1741 tcgagaaccg ccccgaccag actctggttg atgacagcgg tcggaccgcc gccctgggcg
     1801 atgagaacgt tgccttgaac tttcgccatg aactgtctcc gttggtgaga ttaatggaaa
     1861 gccgaatggt tggtgtcagt atgtaccgac gatcgtcatc ggtcaagcca ttccctgcgg
     1921 atcgacagga caatggccag gcatcgcttg tttcactcgc cttctggctc acacaccgaa
     1981 gaagggggtg tgtttg
//
LOCUS       DAORHD010001008         1994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72359_length_1994_cov_3.875709, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001008 DAORHD010000000
VERSION     DAORHD010001008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72359_length_1994_cov_3.875709"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..>1994)
                     /locus_tag="PKO06_18040"
     CDS             complement(2..>1994)
                     /locus_tag="PKO06_18040"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV71611.1"
                     /translation="SQGFSFRTLDSGAPIITQVTARVTSNSSAVVTWTTDKNTSAMVE
                     YGTISGTYVSQVVSTLVWSTSQSVALTGLQAGRTYYYRVRSSDSSGNTTVSAEYSFQT
                     SGSSAPLISTIQVVTPTDTTASITWLTDQPASTQIEYAVAGQNFVQTTLDPTLATTHT
                     VGLSNLTPYTTYRFHVRSRNAAGLESVSQDQIFMTPDQTAPRIYDLFVQNLMATAATL
                     VWKTDESSDTQVEYGTLGYDQITGLNASMLKNHSVTLTGLTENTAYMFRVRSKDQFNQ
                     VGSSTGVFTTYDRTAPVLENVNVSNITSTSARITWTTVEAGTSQVEYGVSTGYGSTEP
                     VLPFPLSYNTNHQVDLVNLSPQTTYHFRIRCVDASGNQATLRDFTFTTSDTVAPYLSG
                     INVTNITATSATVVWITNEAADSTVNYGPLIPYAATATNTSRTSAHAITLTGLLENLT
                     YNFQVSSTDAAGNTTVSDNYSFTTLDRTPPDVTNVAVATVLESGVTITWDTDEEATSK
                     VEWGLTTTYGSSVEVPGTRTMSHSVAISGLAPFTTYHFRVRSKDALGNERISGDYTFT
                     TLDTLLIQNVTVTSLSRTSARLTWTTNAPASCVITYGDTNPPGNGPQPEDTLPWGLTH
                     NVTISLPGAVSGNDYYFIIDARTGSGVTATTGTRYVTIP"
BASE COUNT          401 a          560 c          598 g          435 t
ORIGIN      
        1 cttacggaat cgtcacatag cgagtgccgg tggtggctgt caccccgctg cccgtgcgtg
       61 catcgatgat gaagtagtaa tcattgccgc tcacggcacc gggtaacgag atggtcacat
      121 tgtgcgtcag cccccaaggc agcgtgtctt caggctgcgg gccgttgccc ggcgggttcg
      181 tatcgccata ggtgatgacg cacgaggccg gcgcgttcgt tgtccaggtc agccgggccg
      241 acgtgcgcga cagagacgtc accgtcacat tctgaatcag cagcgtatcg agagtggtga
      301 aggtgtagtc gccagagatc ctctcgtttc caagagcgtc tttcgagcga acccggaaat
      361 ggtaggtcgt gaacggtgcc agcccgctga tcgccacgct gtggctcatc gtacgagttc
      421 cgggcacttc aacactgctg ccataggtcg tcgtcagacc ccattccact ttcgacgtcg
      481 cctcttcgtc cgtgtcccag gtaatcgtca ctccactctc gagcaccgtc gccaccgcca
      541 cattcgtgac atcgggcgga gtgcggtcga gcgtcgtgaa cgagtagttg tccgacaccg
      601 tggtattgcc cgcggcatcc gtcgacgaca cctggaagtt gtaggtcaga ttttccagca
      661 gaccggtcaa cgtgatcgca tgcgcggagg ttcgtgacgt attggtggcg gtggccgcat
      721 acggaatcaa gggcccatag ttcacggtcg aatccgcggc ttcgttggtg atccagacga
      781 ccgttgccga agttgccgtg atgttcgtga cgttgatgcc gctgagatac ggcgccaccg
      841 tatcgctggt cgtgaaggtg aagtcacgca gtgtcgcctg attccccgac gcgtcgacac
      901 atctgattcg gaagtgatag gtcgtctgcg ggctgagatt gaccaggtca acctgatgat
      961 tcgtattgta cgagagcgga aacggcagaa ccggctcggt agagccgtat ccggtcgaca
     1021 ccccatactc tacctgcgaa gttccggctt caaccgtggt ccaggtaatg cgggcactgg
     1081 tcgaggtaat gttgctcaca ttgacgttct cgagaacggg ggcagtacga tcgtacgtcg
     1141 tgaatacgcc cgtggatgac ccgacctgat tgaactgatc tttcgaacga acgcggaaca
     1201 tgtaggccgt gttttcggtg agaccggtca acgtcacgga atgattcttc agcatgctgg
     1261 cgttcaagcc tgtgatctga tcatacccca gagttccata ttccacctgt gtgtcgctgc
     1321 tctcatcggt tttccacacc agggtggcag cagtcgccat cagattctgc acgaacagat
     1381 catagatacg cggcgcggtc tgatccggag tcatgaatat ctgatcctgt gataccgatt
     1441 cgaggccagc ggcattgcgc gatcgaacat gaaaccgata cgtcgtatac ggtgtcaggt
     1501 tgctcagccc aaccgtgtga gtggtcgcca gggtcgggtc gagtgtcgtc tgcacgaagt
     1561 tctggccggc aaccgcatac tcgatctgag ttgaagccgg ctggtcggtc agccacgtaa
     1621 tgctggccgt ggtgtcggtg ggggtcacca cctgtatcgt cgaaatcagc ggcgcactcg
     1681 agcccgaggt ctgaaatgaa tactcggccg aaaccgtggt atttcctgac gaatcgctcg
     1741 agcgcacacg atagtagtag gtgcgaccag cctgcaaacc ggtcagcgca accgactgac
     1801 tggtactcca gaccagggtc gacaccacct gtgacacgta ggttcccgag atcgtgccat
     1861 actcgaccat cgccgaggta ttcttgtcgg ttgtccaggt aacgaccgcc gaagaattcg
     1921 aagtaacgcg ggctgtcacc tgtgtgatga tcggtgctcc cgaatcgagg gttctgaagc
     1981 tgaacccctg cgag
//
LOCUS       DAORHD010001009         1992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72494_length_1992_cov_3.070728, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001009 DAORHD010000000
VERSION     DAORHD010001009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72494_length_1992_cov_3.070728"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKO06_18045"
     CDS             <1..479
                     /locus_tag="PKO06_18045"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV71612.1"
                     /translation="SPESLIEQINAVVFRFLKKKKHMTGVFALLTPETGEVQLISTGH
                     IPSYLVRVTGDIEEVGKPGFPLGMRQNLKFTEQRMVMNPGDTLVLYTDGIVEAVNWNN
                     DQLGFDRWRLALRDLLTTAFDHDIFDRAVQLIQRHTGQRPFQDDVTLLFLHRRPQS"
     gene            502..1137
                     /locus_tag="PKO06_18050"
     CDS             502..1137
                     /locus_tag="PKO06_18050"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV71613.1"
                     /translation="MHSRFPRVVFSAAMLVLLAIALCQITGCGSQTTTAQVSVKGPVM
                     PVQAKLNSSGTFQDAREGDKVEAGGAIRTGENGRSSLLYPDGTEIAIQADTYFEVKAE
                     GFLGRQTQGSAIYKVKPQKAGTKVETPHGVTAVLGTTFLVKVASSGTAVWVEEGTVEF
                     ATAAGEKKAIKAGENLSYSGTGALGTPAPTNPVEQQGLFNPGAPEPALNQR"
     gene            complement(1195..>1992)
                     /locus_tag="PKO06_18055"
     CDS             complement(1195..>1992)
                     /locus_tag="PKO06_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV71614.1"
                     /translation="WRRTCAACGSQSIPQAAFCGGCGRGFSWHDRLKLKWRKMVSLTS
                     QRRLHGLGSGLAFGTLLAVFAFGTMGMSTFKPVEPVWEKAQTSGVAPAEVGRRILTAW
                     NRSSENLDLERMVTKDDLVRLGNLLLETCSHAIPADQPAGHVPIADSERYLQEFARVE
                     DGILSTEISRSDAVVFLFRIVNDLFEITPAEETTYKYSDIPRYHYLNLPVETLENLGL
                     KLSRNELEFGGDDQVSLAWLSEVTKSLVKVLDQRLKDKRSVSLDVQH"
BASE COUNT          447 a          583 c          568 g          394 t
ORIGIN      
        1 tgtcgccgga atcactgatc gaacagatca acgccgtcgt gttccgtttt ttgaagaaga
       61 agaaacacat gacaggagtc tttgccctgc tcacgcccga gaccggcgag gtgcagctga
      121 tcagtaccgg gcatattccc agctatctcg tgcgtgtcac cggcgacatc gaagaagtcg
      181 gcaaacccgg gtttccactg ggaatgcgcc agaacctgaa attcaccgaa cagcgcatgg
      241 tcatgaaccc gggagacact ctggtactct ataccgatgg tattgtcgag gccgtcaact
      301 ggaacaacga tcagctcgga ttcgaccgtt ggcgcctggc tctgcgagac ctgttgacca
      361 ccgccttcga tcacgatata tttgatcgag cggtgcaact gattcagcgc cacacagggc
      421 agcgaccgtt tcaagatgat gtgacgctgt tgttcctgca tcgacgacca cagtcctagt
      481 gtcagacaag ggaggatcct catgcattca cgttttcccc gcgtcgtttt ttcggcggcc
      541 atgctggtcc tgctggctat tgcgctctgt cagatcactg ggtgcggcag tcagacgacc
      601 acagctcagg tttcagtaaa gggaccggtg atgccagtcc aggcgaagtt gaactcctct
      661 ggaacctttc aggatgcccg ggaaggcgac aaggtggagg ccggtggcgc cattcgaacc
      721 ggcgaaaacg gacgatcgtc gctgctgtat cctgatggca ccgagatcgc tattcaggcc
      781 gacacctatt tcgaagtcaa ggccgaaggt tttctgggtc ggcagacgca gggctcagcc
      841 atctacaagg tgaaacctca gaaggccgga accaaggtcg agactccgca cggcgtcacg
      901 gcggttctgg gcacgacgtt tctggtcaaa gtcgcgagca gcggtaccgc agtctgggtc
      961 gaggaaggca ctgttgaatt tgcgacggcc gcaggcgaaa agaaagccat caaagctggc
     1021 gaaaacctga gttactcagg aaccggagct ctcggcacgc ccgcaccgac caacccggtc
     1081 gagcagcagg ggctcttcaa ccccggagca ccagaacccg cattgaatca acgatagcgc
     1141 gcccaagaga agcgcccccg ccggccattg ccagcggggg cgcttgtttt tgcgtcagtg
     1201 ctgaacgtct agagacacgc ttcgtttgtc tttcaacctc tgatcaagca ccttgaccag
     1261 gctcttcgtc acttccgaga gccaagccag cgatacctgg tcgtcgcccc cgaattccag
     1321 ttcattgcga ctcagcttca gccccaggtt ttcgagagtc tccaccggca gattcagata
     1381 atgataccgg ggaatgtcgc tgtatttgta ggtggtttct tctgccgggg tgatctcgaa
     1441 cagatcgttc acgatgcgaa acagaaagac cacggcgtcc gatcgggaaa tttcggtcga
     1501 gaggatgcca tcctcgacac gcgcaaactc ctgaagatag cgttctgagt cggcaatcgg
     1561 cacatgtccg gcgggctgat cggcaggaat ggcgtgtgag caggtttcga gcagaagatt
     1621 ccccaggcgt accaggtcat ccttcgtaac catgcgctct agatcaaggt tttcgctcga
     1681 tcgattccag gcagtgagaa tgcgtcgccc aacttcggcc ggtgcgacgc ccgacgtttg
     1741 cgctttttcc cacaccggct cgaccggctt gaacgtgctc atgcccatgg ttccaaaggc
     1801 aaaaaccgcc agcagggttc cgaaggccaa tcccgagccg agcccgtgca ggcgacgctg
     1861 ggaagtcaac gagaccatct tgcgccattt caatttcagg cggtcatgcc aggaaaaacc
     1921 gcgaccgcaa ccaccacaga aggccgcctg aggaatactc tgcgaaccgc aggccgcgca
     1981 ggtgcggcgc ca
//
LOCUS       DAORHD010001010         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72710_length_1988_cov_2.909467, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001010 DAORHD010000000
VERSION     DAORHD010001010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72710_length_1988_cov_2.909467"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PKO06_18060"
     CDS             complement(<1..731)
                     /locus_tag="PKO06_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71615.1"
                     /translation="MKHRLVVVLVLLVMVAGASIPAFAARNPNRLAVEARTLIAKAIG
                     LSESDPVTSGNLFRKAFENAVTLTQPKMGQFWQETGLALASRCLHPDLFPEVRIAAET
                     YREKFPAGRRVREVLVSLALLEAAEGNDRQALALLADAAGRAQGNERLKTEALGLDAA
                     LGAGYYRSAEQLLTELNRDRKVRRFKRDQKRFDNGTALLNAALKEVVEGRVAGEAAVR
                     TLRSALEAGYFAEEAPAAELLALGE"
     gene            complement(760..1917)
                     /gene="yihA"
                     /locus_tag="PKO06_18065"
     CDS             complement(760..1917)
                     /gene="yihA"
                     /locus_tag="PKO06_18065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNV71616.1"
                     /translation="MSLKITSARFTACVTSRAGIPDDGLPMIALVGRSNVGKSSMINT
                     LSGRREMAKTSATPGKTLTCNFYLINESFYLVDLPGYGYAKVSRVTRERIQTMVDEFF
                     TDNTRLKGIIQIMDLRHPPTNMDVSMFNWIRGGGSRYLPLVTKADKLGQGEMHRMFKL
                     YRQKMDAPGMIVFSAKTGQGKDDLLDAVRAMINDEEFAAPRMRMPDRGAQKRGRGGRD
                     RQDGRRDNRDNRDNRRPAPKDPRPSAGNESLPGSTPQPQAASGEMEPRSPRDPRPQAQ
                     SSDQRPRPPQDRRPQTGGRDGQGGRPGRGDRPQGSSHPRPQNQSGDAAGRSTGQGDQA
                     PERPAQGQGGGRRHRRPHHRRPDRDQSPRDGGSRPGPGSDSGPKSGSDSAT"
BASE COUNT          357 a          646 c          565 g          420 t
ORIGIN      
        1 tcttcgccca gcgccagcaa ctcggctgct ggcgcctctt ccgcgaagta gccggcttcg
       61 agcgccgaac gcaacgtgcg aaccgcggct tcacccgcga cccggccttc caccacttct
      121 ttcagggcgg cattcagcag cgctgttccg ttgtcgaacc gcttctggtc gcgcttgaag
      181 cgccgcactt tgcgatcccg gttcagttcg gtcagcagct gttcggcgga gcgataatat
      241 ccggcgccca gtgcggcatc gagcccaagc gcttcagtct tgagacgctc gttgccctgt
      301 gcccgacctg ccgcatcagc caacagcgcc agtgcctgtc gatcattgcc ttcagcggct
      361 tcgagcagag ccagcgaaac cagcacctca cgcacccggc gacccgcggg aaacttctcg
      421 cgataggttt ccgccgcaat ccgcacttcg ggaaacagat cagggtgcag gcagcgagag
      481 gccaaagcca gtcccgtttc ctgccagaac tgccccatct tgggctgcgt cagggtgacg
      541 gcattctcaa aggcttttcg gaagagattt ccggaggtga ccgggtcgga ctcactcaga
      601 ccgatcgcct tggcaatcaa cgtgcgcgct tcgaccgcca ggcggttcgg attccgcgcc
      661 gcaaaggccg gaatcgaagc accggcaacc atcaccaaaa gaaccaggac cactaccaat
      721 ctgtgtttca tagcaacctc tcgtgtaaag gtttcttcgt cacgtcgctg aatctgaacc
      781 cgacttcggt ccggaatcgc ttccgggtcc cggtcgcgaa ccgccatcac gtggggactg
      841 atcgcggtcc ggtcgacgat ggtgcggacg tcgatgacgc cgccccccac cctgtccctg
      901 agccggtcgt tccggtgcct ggtcgccctg gccggttgag cgcccggccg catcaccact
      961 ctggttctgc ggtcgcggat gcgaagaccc ctggggtcga tctccacgac cgggtcgtcc
     1021 accctgccca tctcgcccac cggtctgtgg gcgacgatcc tgcggcggac gcggtctctg
     1081 atctgatgac tgcgcctgcg ggcgtgggtc gcgcggtgat cgaggttcca tctcgccact
     1141 cgcagcctgt ggctggggag tagagcccgg tagggattcg ttgcccgctg aaggacgggg
     1201 atctttgggc gccggtcgtc gattatcccg attatcccga ttgtcgcggc ggccatcctg
     1261 acgatcgcga ccgcctcggc cgcgcttctg cgctccgcgg tccggcattc gcatgcgcgg
     1321 agccgcgaac tcttcgtcat tgatcatcgc ccgcacggca tcgagcagat cgtccttccc
     1381 ctgaccggtt ttggcggaaa acacgatcat gcccggcgca tccatcttct ggcgatagag
     1441 cttgaacatg cgatgcattt cgccctgtcc cagtttgtcg gcctttgtca ccagtggtag
     1501 atagcgtgag cccccaccac gaatccagtt gaacatactg acatccatat tggttggggg
     1561 atgacgcaga tccatgatct gaataatgcc cttcagccgc gtgttgtcag taaaaaactc
     1621 gtcaaccatg gtctgaatgc gttcacgggt cactcgcgaa accttggcat acccgtagcc
     1681 cggcagatcg acgagataga agctctcatt gatgagatag aaattgcagg tcagtgtttt
     1741 cccaggtgtc gcgctggtct tggccatctc acgacgacct gacagcgtat tgatcatcga
     1801 ggatttcccg acgttactac gccccacgag cgcaatcatc ggcaacccat cgtcgggaat
     1861 accggctctg ctcgtgacac aggctgtaaa tcgagccgaa gtgattttca gcgacatgtc
     1921 tctacctggc ccccgcggag tctgatctgc gtttgctatt tgcagatttt ctccgtctcg
     1981 gttcgatg
//
LOCUS       DAORHD010001011         1988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_72716_length_1988_cov_2.758407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001011 DAORHD010000000
VERSION     DAORHD010001011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72716_length_1988_cov_2.758407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1988)
                     /locus_tag="PKO06_18070"
     CDS             complement(<1..>1988)
                     /locus_tag="PKO06_18070"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV71617.1"
                     /translation="DPNNLDLVLTSTTLQKATIDGETIVSANEPVIQSVTPAAGSTGV
                     ATNTTITATFNTPMSLSALQNNNFKVVTPGVGIGGGGGLEVPVSGTITMSGNTAVFTP
                     TAPFAYGTLVTVTIATGTIDQTGKALLTQGKTWSFTTGSPGTPANFAKGLDQTVLEDA
                     AAQNITGWATSISVPTGNPVFTVTNNNNALFRTQPTISPTGVLSFWPAADANGQALLT
                     ITLNSDAAGVTPSTPQLATITVTPVNDPPSFVKGPDVYKINQAGQVTIPNWMTGITAG
                     PADEGSQAVTISVSANSALAFTTQPTIGPTGTLSFEPNVTLISGQGLFNNTMTITCAD
                     NGGTANGGSNTSAAQAAKIVFTDPVISLLHSNPDVNGNSKIGTDTIITLTFDQPIDLT
                     TLVNQLSITYTVGQPEKFTLADYSQTGSTVYLTVPGGLQVVGPAMLDIAAGVNGVSGA
                     TLTAAISINFEICSTDNNIVTPTITAPYDNETMIGTKPSIGATNIIGYTLYSSEWEIY
                     ESSTISTANRIFYGYENSSNPLINTSDGSGLYLNSHSDLNELKPGTDYWVRVRYIAFS
                     TTPPPYYVSEWSPLSKFTTSFPPLAPTIIVPISPTGLNPTLDSTAFSDPDSSDSLQYS
                     EWQIFEDADMSLASNQIWNSSLGGDTAVNVDQSDGYFM"
BASE COUNT          405 a          471 c          636 g          476 t
ORIGIN      
        1 catgaagtaa ccgtcagatt ggtcaacgtt cactgccgtg tctcctccga gagacgagtt
       61 ccagatctga ttgctggcta agctcatatc cgcgtcttcg aagatctgcc actcggaata
      121 ctgcagcgaa tctgatgaat ccggatccga gaacgcggtc gaatcgagag tcggattcag
      181 acccgtcggg gagattggaa cgatgatcgt gggcgcaaga ggagggaatg aggtggtgaa
      241 cttgctgagc ggcgaccact cgctgacgta atacggcgga ggagtggttg aaaacgcgat
      301 gtatcgcacg cgaacccaat agtctgttcc gggtttcagt tcattcagat cactatgact
      361 gttaaggtat aagccgctcc cgtcagaggt gttaatcaaa gggttgctac tgttttcgta
      421 accgtaaaag atgcgattgg cggtagaaat ggtcgagctt tcgtagatct cccattcaga
      481 gctgtagaga gtatagccga tgatgttggt tgcgccgata cttggctttg ttccgatcat
      541 tgtctcgttg tcatagggag cggtaatggt gggagtgaca atgttgttat cggtcgagca
      601 gatctcaaag ttgatggaaa tggctgcggt cagcgttgcg cccgagaccc cgttgactcc
      661 ggctgcgata tcgagcatgg caggaccaac gacctgcaac ccgccgggaa cggtcagata
      721 gacagtggag cctgtctggg aatagtctgc aagtgtgaat ttttcgggtt gtccaacggt
      781 ataggtgata cttaactgat taaccagcgt agtcagatcg atcggttggt cgaaggtgag
      841 cgtgatgatc gtgtcggtac caatcttgga attaccgttg acatctgggt tgctatgaag
      901 cagagagatg acaggatcag taaacacgat tttggctgcc tgggcggcag acgtgtttga
      961 gcctccattc gcagttccgc cattgtctgc acaggtgatg gtcatggtgt tgttgaagag
     1021 accctgaccc gaaatcaaag tgacatttgg ctcgaagctc agagtgcccg ttggaccgat
     1081 tgtcggttgc gtcgtgaagg cgagagcact gttcgctgaa acactgattg tcacggcttg
     1141 gctgccctca tccgccggtc cggcagtgat ccccgtcatc cagttcggga tggtcacttg
     1201 tccggcttga ttgatcttgt agacatcggg acctttgacg aagctgggtg gatcgttgac
     1261 aggggtgacc gtgatcgtcg ccagttgcgg ggttgaggga gtgacaccgg cggcatcgga
     1321 gttgagcgtg atggtcagca gggcctggcc gttggcgtct gcggccggcc agaagctgag
     1381 aacaccggtg ggggagatcg tgggctgagt tcggaaaaga gcgttgttat tgttcgtgac
     1441 cgtgaaaacc gggttcccgg ttggtacaga aatcgaggtc gcccagccgg taatgttctg
     1501 agccgcggcg tcttcaagaa ccgtttgatc caggcctttg gcaaagtttg cgggagtccc
     1561 cggcgagccg gtcgtgaagc tccaggtctt gccctgggtc aacagcgctt tgccggtctg
     1621 atcgatggta ccggtggcaa tggttacagt gaccagcgtc ccgtaggcaa agggggcggt
     1681 gggtgtaaag acggcggtgt taccggacat cgtgatggtg ccgctcaccg gcacttcgag
     1741 cccgcctccg cctccgattc cgaccccggg cgtgacaacc ttgaagttgt tgttctgcag
     1801 cgcggacaaa ctcatcggcg tgttgaaggt ggcggtaatc gtcgtgttgg tggcaacgcc
     1861 cgtgctgccg gccgccgggg tcacgctctg tatcaccggt tcgttggccg aaacgatggt
     1921 ttctccgtca atcgtcgctt tctgcaacgt cgtgctggtc agcaccagat cgaggttgtt
     1981 ggggtcat
//
LOCUS       DAORHD010001012         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73046_length_1982_cov_2.675662, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001012 DAORHD010000000
VERSION     DAORHD010001012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73046_length_1982_cov_2.675662"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="PKO06_18075"
     CDS             <1..578
                     /locus_tag="PKO06_18075"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNV71618.1"
                     /translation="GGQGHAPCDEFRHLLPGRKARVPWERLLRMFAGQALAKDDYSLA
                     RPNKRYLDQDIVVPGLFSESLSLVVVSLDTSGSMDDETIASGLAEIAKLHDLAPEILL
                     IVGDDDVHETIETQNLPAYVKDLRVKGGGGTSHLPVFRYIERNRLTPDLFIGITDLES
                     CFPSQPPRYPVLWLVPTEHDKAPWGRVIEIE"
     gene            602..859
                     /locus_tag="PKO06_18080"
     CDS             602..859
                     /locus_tag="PKO06_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71619.1"
                     /translation="MSNPVINIKCMLHYSDDPCDIQRCLDDLYAYLRTQPCMSDFPRL
                     NDFSGIDNSYEFDQLLADLYQYCDAHSITVDWKRVDYFLTK"
     gene            1283..1510
                     /locus_tag="PKO06_18085"
     CDS             1283..1510
                     /locus_tag="PKO06_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71620.1"
                     /translation="MSNPVINIKYMMHDSDDPCDIGRCLDDLHAYLSAQPCMADFARL
                     NDFCGIDNSYEFDQLLSDLFQYCDAHGIVID"
     gene            1542..1961
                     /locus_tag="PKO06_18090"
     CDS             1542..1961
                     /locus_tag="PKO06_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71621.1"
                     /translation="MKRRTKSNKQVREPQLKIMDYACPTDDEVVTFIGGPVEVMHVRD
                     GEHDHLILYHRDGFRSGAPLNKFASWVANQSIYGNAILLVDDGQWTDFILYAQQTRSE
                     KRGCFHVKTFHQFRRTENSQGSTAEGEKLSATDRKEP"
BASE COUNT          474 a          554 c          490 g          464 t
ORIGIN      
        1 tgggtggtca ggggcatgcg ccgtgcgatg agtttcgtca ccttctgccg ggtcgcaaag
       61 cccgcgtgcc gtgggagcgg cttctcagaa tgttcgccgg acaggcgttg gcgaaagacg
      121 actattcact ggcgcgaccg aacaagcgat atctcgacca ggatatcgtc gttccggggt
      181 tgttttccga atcgctcagc ctcgtcgtag tctcactcga cacctcgggc agtatggacg
      241 acgagaccat tgccagcggc ctggccgaga tcgccaaact gcatgacctg gcccccgaga
      301 ttctgctgat cgtcggtgat gacgacgttc acgaaaccat tgagacgcag aacctgccgg
      361 cctacgtgaa agacctgcgc gtcaagggcg gcggcggcac cagccatctg cccgtgtttc
      421 gctacatcga acggaatcgt ctcaccccgg atctgttcat cggtatcacc gatctcgaat
      481 catgctttcc gtctcagccg ccgcgttacc ccgtgctgtg gctggtacca acggaacacg
      541 acaaagcccc ctggggccgc gtcatcgaga tcgagtgaca ggggaaacaa agggggacgg
      601 tatgtctaac cccgtgatca acatcaagtg tatgcttcat tacagcgacg atccctgcga
      661 tattcagcgt tgtcttgacg atctctacgc atatctccgc actcaaccct gcatgtcaga
      721 ctttccgcgt ctcaacgact tcagcggcat cgacaactcg tacgaatttg accaactgct
      781 cgccgacctc taccagtact gcgatgccca cagcatcacc gtcgactgga agcgcgttga
      841 ctatttttta accaaatgaa caaaagtcac gactttcttt caactgggaa aaattaagtc
      901 gtcacctgag tccgctcgac ctggaaggta tcatcgttct cttcgggaaa aagcatgagc
      961 ggaggaagaa ttctctcccc ccgctctgta tagccagtga gggcaacaag ctgtttggag
     1021 atattcggtg taatccccag ttcatttgcc ctcagaactc ccgcttcacg tcttccgccg
     1081 ttccttcgac caccggtcac tcatacctat tccggaaaaa acacagtaca cttgaaattt
     1141 cgttttgatt cccccttttt gattgtccct tgcggctggc cgcccgaccg ctgttcgtga
     1201 tcattgaaac gtacaaccgt tgttttccgg aatgtcatgg atagcggcga cccaatatca
     1261 taagcgaggc aaggaggaag gtatgtccaa tcccgtgatc aacatcaagt acatgatgca
     1321 tgacagtgac gacccctgcg atatcggtcg atgtctcgac gatctgcacg cttatctcag
     1381 tgctcagccc tgcatggcag actttgctcg tctcaacgat ttctgcggca tcgacaactc
     1441 gtacgaattc gatcaactgc tctcagatct cttccagtac tgcgacgctc acggtatcgt
     1501 catcgactga tgtctcggta tttctatcag aggggcacat catgaaaagg cgaacaaaaa
     1561 gcaacaagca ggttcgtgaa ccccagctca aaatcatgga ctacgcctgc ccaacggacg
     1621 atgaggtggt tacattcatc ggcggtccgg tcgaggtcat gcacgtgaga gatggcgagc
     1681 atgatcattt gattctctat catcgtgatg ggtttcgcag tggcgctcca ctcaacaagt
     1741 ttgcttcctg ggtggcgaat caatcgattt acggaaatgc tattcttctc gttgacgacg
     1801 gacagtggac ggattttatc ctgtatgcgc aacagactcg ttccgaaaaa aggggatgtt
     1861 tccatgtcaa gacctttcat caatttcgaa ggacagaaaa ttcacaggga agcacagcag
     1921 aaggtgaaaa gttgtctgca acggatcgca aagaacctta aatcctacaa cacattggca
     1981 aa
//
LOCUS       DAORHD010001013         1981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73086_length_1981_cov_3.403946, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001013 DAORHD010000000
VERSION     DAORHD010001013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73086_length_1981_cov_3.403946"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1981
                     /locus_tag="PKO06_18095"
     CDS             <1..>1981
                     /locus_tag="PKO06_18095"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV71622.1"
                     /translation="TDCRSFRPCSPDQQAELARFGSHNLQNTPGADFIGGIEEPLACG
                     QTLNTGTLSLPVFPFLDTLELFTGFSPRKMTDELKRDLVFILVGAHTDIRVYHTAFTL
                     RHDYQFPHVVVCPHLVGSPNHLGHEQYLQANYSDALIRVVSDLRDVYTIAGFEAAAPI
                     AGAHQGCRFQPADRFASLSPDYQEVLKRLFLFQAVVELKQLGGGYSGSLLVIATGTDH
                     HGARLAPVIVKIDRHDKIQREIEGYHRVNHLIGNHVPHFHTPVSHGEATGIRMDVATM
                     SGLPQTFQRRFETAADQAAADRFLRLLRGALTGLIDRVIANRRHERRIYPYRELGLHT
                     RQQLLWLTDNLSQIIPTYSPSAKVLSLVDKTVIANPYPLYEELVGHADRLEVPVSLCH
                     GDLNLANLITDDSDNWWIIDWSYAAEGPVERDLAKLENDLKFVLMREVVESDYPRLLC
                     FETFLQSTFALPALEHLPQSLRWLSESTRWQLLYQSLREVREAAIRAGGPQRVSEMYD
                     LFLVKFAAHTLSFDQRRVNPHTGQPRGECSVPMMTWAAISLSLLLGKLKASPWHTRVL
                     RDRLAGYPERFPVPIEKRSWSVSFPEYRPPWHVIPAVLANDGTKVPSGWADPEDHLRI
                     PDLRQRQSLSGTLNFDTEGRPLNPSGRTGIAGRGF"
BASE COUNT          378 a          633 c          548 g          422 t
ORIGIN      
        1 ccacagattg tcgatcgttt cgtccttgtt cgccggatca acaggcggaa ctggccagat
       61 ttgggtcgca caacctgcag aacacccccg gagccgattt catcggtggc atcgaggaac
      121 cgttggcctg cggccagaca ctgaataccg gaactctcag tctacccgtc ttcccattcc
      181 tcgatacgct cgaactgttc accggattct ctccacgcaa gatgaccgat gagctcaagc
      241 gtgatctggt cttcattctc gtcggagctc acacggacat tcgcgtctac cacaccgcgt
      301 tcaccctgcg gcatgactat cagtttcccc atgtcgtggt ctgcccgcat ctggttggaa
      361 gccccaacca cctgggtcac gagcagtatc tgcaggccaa ctactcggac gctttgattc
      421 gcgtggtgag cgatctgcgc gatgtctaca ccatcgccgg attcgaagct gcggctccga
      481 ttgccggggc gcatcagggc tgtcggtttc agcctgccga ccggtttgcg agtctctcgc
      541 ccgactatca ggaagtgctg aagcgtttgt tcctgtttca ggccgtggtc gagttgaaac
      601 agctcggcgg cggatacagc ggcagtctgc tcgtcatcgc caccggcacc gaccatcacg
      661 gcgcacgact ggcccccgtc atcgtcaaga ttgatcgtca cgacaagatc cagcgggaga
      721 tcgagggata ccatcgcgtc aaccacctga tcgggaacca tgtcccccac tttcatacgc
      781 ctgtctcgca cggagaggcc accgggatcc gcatggatgt cgccacgatg tcgggcctgc
      841 cgcagacgtt tcaacggcgt tttgaaacgg cggcggacca ggcggcggcc gatcgcttcc
      901 tgcgcttgtt acgcggggca ttaaccggat tgattgaccg cgtgattgcc aaccgccgtc
      961 acgagcgccg catctatccg tatcgcgaac tcggcttgca cacgcgacag caactgctct
     1021 ggctcacgga caatttgtcc cagatcatac caacgtattc gccctcagca aaggttcttt
     1081 cgcttgttga caagaccgtc atcgccaacc cgtacccact ctacgaagaa ctcgtcggtc
     1141 atgccgaccg tctcgaagtg cctgtctctc tctgtcacgg cgatctgaac ctcgccaacc
     1201 tcatcacgga cgacagcgac aactggtgga tcatcgactg gtcgtatgcg gcagaagggc
     1261 cggttgagcg cgatctcgcc aagctcgaaa atgatctcaa attcgtcctg atgcgagagg
     1321 tcgtcgagtc cgactatccg cgtctactct gtttcgagac atttctgcag tcaaccttcg
     1381 ctctgccggc tctcgagcac ctgccacagt ctctgcgttg gctgtccgag tcgacacgat
     1441 ggcaacttct gtatcaaagc ctgcgagaag ttcgagaagc ggcgatcaga gccggcggcc
     1501 cacaacgggt gtcagagatg tatgacctct ttctggtcaa attcgccgct catacgctca
     1561 gcttcgacca gcgccgcgtc aatccgcata ccgggcagcc ccggggggag tgttccgtgc
     1621 ccatgatgac ctgggcggcc atctcgttga gtctgctttt gggcaagctg aaagccagcc
     1681 cctggcacac gcgggtgctg cgcgatcgtc tggcgggcta tcccgagcga ttccccgtcc
     1741 ccatcgaaaa gcgttcctgg agcgtgtcgt ttcccgagta tcgccccccg tggcatgtca
     1801 tccccgcggt gttggccaac gatggcacca aggttccgtc aggatgggcg gatcccgaag
     1861 atcacttgcg cattcccgat ctgcggcagc gacagagtct gtcgggtacg ctgaacttcg
     1921 ataccgaagg acggccgttg aacccaagtg gtcgcaccgg catcgccggt cgtggattct
     1981 g
//
LOCUS       DAORHD010001014         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73266_length_1978_cov_3.549662, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001014 DAORHD010000000
VERSION     DAORHD010001014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73266_length_1978_cov_3.549662"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="PKO06_18100"
     CDS             complement(<1..604)
                     /locus_tag="PKO06_18100"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNV71623.1"
                     /translation="MTPINPNVNQTVTFQHGQVAELLRRLERFLGIPLSLRSNTGEVV
                     VKTDYFFGPCSIIRGTEVGRLRCQKTYRNIEDHLLYRKVPYVNICYAGFLIFAVPLEF
                     QGEMIGTLFGAQILPIRLARKTEILEQFQESATAVGIRDLEEFCRSFDRVRNLEPDFQ
                     RISFLEFLQKIGENFISMALAGKSWPQFYREIRDELKILER"
     gene            complement(640..1728)
                     /locus_tag="PKO06_18105"
     CDS             complement(640..1728)
                     /locus_tag="PKO06_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNV71624.1"
                     /translation="MNLLDRMSEHDYEQVLFALDDPSGLKAIIAIHDTTLGPAMGGVR
                     FKAYATEEEALKDVLQLSRAMTLKASAAGLNLGGGQAVILADSAAGKSEALLRAFGRQ
                     IQALSGRLFVAEDLGTTVEDMETIQQETEYVAGLRKSRGGSGEPSLKAAYGVIRGIGA
                     CLLHVHGDPGLRGRVLAIQGVGAVGREIARQASEEGARVIVADKDPRRIDDLKKHFEV
                     QVVSPDHILETECDVFCPCALGGVLSETTVPRLRAKIVAGAANNQLVDENAGKLLFER
                     GIIYAPDFVISAGGLINVAEELTGYDENLATLRVGKLYQRIEAILKVSEERKIPTHLA
                     AAQMALERLELMRKVRRTYVPNASSLDR"
     gene            complement(1731..1952)
                     /locus_tag="PKO06_18110"
     CDS             complement(1731..1952)
                     /locus_tag="PKO06_18110"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNV71625.1"
                     /translation="MFVTPRNETGNVLYKSLQYYARTPMGGLVFGAKCPVVLTSRADD
                     NETKFASLLLGVLLWQSAQTGASTKAMEA"
BASE COUNT          422 a          616 c          532 g          408 t
ORIGIN      
        1 atcgttcgag aattttcagt tcgtcgcgaa tctcgcgata gaactggggc caggacttgc
       61 cggccagcgc catcgagatg aaattttcgc cgatcttctg cagaaactcg agaaacgaaa
      121 tgcgctggaa gtcgggttcc aggttgcgca ctcgatcgaa cgaacggcag aactcttcca
      181 ggtcacgaat gcccaccgct gtcgccgact cctgaaactg ttccagaatc tccgttttgc
      241 gtgccagtct gatcggcagg atctgcgcgc cgaacagggt gccgatcatc tctccctgaa
      301 actcgagggg aaccgcaaag atgaggaatc cggcgtaaca gatattgacg tagggaacct
      361 tgcgatacaa cagatggtct tctatattgc gataggtctt ctgacagcgc agccggccga
      421 cctcggtgcc gcgaatgata ctgcagggcc cgaaaaagta atcggtcttg accacaacct
      481 cgcccgtatt cgatcgcagc gacaagggaa ttccaagaaa ccgctcgaga cgacgcagca
      541 gctcggccac ctgtccatgc tgaaaagtca ccgtctgatt gacattcggg ttgatcgggg
      601 tcatgagtcc tctcctttca tgcgctctcg aacgtgtcct taccggtcca ggctactggc
      661 gttgggaaca tacgtgcggc gaaccttccg catcaattcc agccgttcga gcgccatctg
      721 agctgccgcc agatgagtag gaatttttct ttcttcggaa actttcaaaa tcgcttcgat
      781 gcgctgatac aacttgccga cccgcaatgt cgccaggttc tcgtcatagc cggtcagctc
      841 ctctgcaaca ttgatcagac cgccggcact gatgacaaaa tcgggggcgt agatgattcc
      901 ccgctcgaac aacagcttcc ccgcgttctc gtcgacgagc tggttgtttg ccgcacctgc
      961 cacgatcttc gcgcgcaggc gaggaaccgt ggtttcgctg agcacgccac cgagcgcaca
     1021 ggggcagaag acatcacatt ccgtttcgag aatgtggtca ggcgacacga cctgcacctc
     1081 gaaatgtttc ttgagatcgt cgatgcgacg tggatctttg tcggccacga tcacccgtgc
     1141 gccttcttcg ctcgcctgcc gcgcaatttc acgccctacc gcgcccactc cctgaattgc
     1201 cagcacgcga ccgcgcagcc ccgggtcccc gtgaacgtgc agcagacagg ccccgatccc
     1261 gcggatgacc ccataggccg ccttcagcga cggctcacca ctgccgcctc ggctcttgcg
     1321 cagaccggcg acgtactcgg tctcctgctg gatcgtctcc atgtcctcga cggtcgtgcc
     1381 gagatcttcg gcaacgaaca agcgcccgga caacgcctgg atctgccggc caaaggcgcg
     1441 cagcagtgct tccgacttgc ctgccgcgga atcggccaga atgaccgcct gaccaccacc
     1501 cagattgagc cccgcggccg aggctttcag agtcatggcg cgactcagct gtaaaacatc
     1561 tttgagagct tcttcctcag tggcataggc cttgaatcgc acgccaccca tggccggtcc
     1621 gagcgtcgtg tcatgaatag cgatgatcgc tttcagaccg gaaggatcat cgagcgcgaa
     1681 gagcacctgc tcgtaatcat gttccgacat tcggtccagc agattcatgg tcacgcctcc
     1741 atggctttcg tggaagcacc cgtttgagcg ctctgccaga gcagcacacc gagcagcagc
     1801 gacgcgaatt tcgtttcatt atcgtcagca cgcgaagtca agacgacggg acacttcgcc
     1861 ccaaacacca gcccgcccat gggggtccgg gcgtaatact gcagtgattt gtacaagacg
     1921 ttgccggttt cgttgcgggg agtgacgaag atgtcaacgt cttgtacaaa tcactgca
//
LOCUS       DAORHD010001015         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73320_length_1977_cov_3.784079, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001015 DAORHD010000000
VERSION     DAORHD010001015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73320_length_1977_cov_3.784079"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /gene="recA"
                     /locus_tag="PKO06_18115"
     CDS             complement(<1..814)
                     /gene="recA"
                     /locus_tag="PKO06_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNV71626.1"
                     /translation="MSDPKAAVSRGLPERNSEKLKALETAVSQIERQFGKGAVMRLGD
                     SRHLQVEVIPTGSLSLDIALGVGGMPRGRIVEIYGPESSGKTTLALQVVAEAQKAGGQ
                     AAFIDVEHALDPSYAKLIGVDVENLLIAQPSSGEEALEIADMLVRSNAIDVIVVDSVA
                     ALTSKAELEGDMGDASVGMQARLMSQAMRKLTPSVSRSRGCCIFINQIREKIGVMFGN
                     PETTPGGRALKFFASVRLDIRRKEQLKEGEEPIGYITEVKVVKNKVAPPFRKA"
     gene            complement(933..>1977)
                     /locus_tag="PKO06_18120"
     CDS             complement(933..>1977)
                     /locus_tag="PKO06_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71627.1"
                     /translation="SQGGHHPQPPTSLQRIRLLRLPTRRTFLLQELAGDTSATPTAAL
                     AISPAVAPPPASTPTPSRAGRILIVEDNPVNTLVASRILEKAGYAVATAENGRIAVDT
                     VRTSAFDLIFMDGQMPEMDGFMATSQIRRMTDIKQPVIIGLTALASKADRDRCLAVGM
                     DDYLAKPITAKLLLERAEHFLGATPPTGPAPAAVPASSGGSPSSAIPAATTPAPVPTI
                     STTAATEAPVDTSAEPPLFEPQKVLENLGGDTVAFQYLAGVFRQTTNQHLTRLAEQVA
                     ARSSEASRTAHSIKGSALEMGASKLAAAALQLEKLCREERFVEAEAAISELGALWQAI
                     DTALQAHLKTLIH"
BASE COUNT          371 a          535 c          675 g          396 t
ORIGIN      
        1 gggccttgcg gaatggcgga gccaccttgt tcttgaccac tttgacttcg gtaatgtagc
       61 cgatgggttc ttcgccttcc ttgagctgtt ccttgcggcg aatgtcgagt cgaaccgagg
      121 caaagaactt gagcgcgcga ccgcccggcg tggtttcggg gttgccgaac atgacgccga
      181 tcttctcgcg aatctggttg atgaagatac agcagccgcg agagcggctg acagaggggg
      241 tcagtttgcg catcgcctgg gacatcaggc gcgcctgcat accgactgac gcgtcgccca
      301 tgtctccttc gagttcggct ttgctggtca aagccgcgac ggagtcgacg acgatgacgt
      361 caatggcatt gctgcgtacc agcatgtcgg cgatttcgag cgcttcttca ccgctgctgg
      421 gctgagcgat cagcaggttc tcgacatcga cgccgatcag cttggcgtaa ctggggtcga
      481 gggcatgttc gacgtcgata aacgcagcct ggccgcccgc tttctgggct tcagcgacta
      541 cctgcagggc cagggtcgtt ttccccgagg attcaggacc gtagatctcg acaatacgac
      601 cgcggggcat gccgccgacg ccgagagcga tgtcgagcga gagagagccg gtcggaatga
      661 cttcgacctg cagatggcga gaatcgccga gacgcatgac cgcccctttg ccgaactggc
      721 gttcgatctg agagacggcg gtttcgagag ccttgagttt ttcagagtta cgttcgggca
      781 gaccgcgtga cacagcagct ttcggatcag acatgagttt gcctccaata atgaatctct
      841 gaggcgagag tagcacaaat ggcgggggtg ggtcaaaaag cgggtaccct gctgaatcgc
      901 ctgtgagccc gatcgagaga aggatggaaa agtcagtgaa tcagggtttt cagatgcgcc
      961 tgcagggcgg tgtcgatggc ttgccagagc gcgcctaact cggaaatggc ggcttcggct
     1021 tcgacaaaac gttcttcgcg gcacaacttc tcgagttgca gagccgctgc cgccagtttt
     1081 gacgcgccca tttcgagtgc gcttcccttg atggagtgag cggtgcgcga ggcctcggaa
     1141 ctgcgggcgg ccacctgctc cgccagccgt gtcaggtgct gattggtcgt ctgtctgaag
     1201 actccggcga gatactgaaa agccaccgtg tccccaccga ggttttcgag caccttctgc
     1261 ggctcaaaca acggtggttc ggccgatgtg tccacgggag cttcggttgc cgcggtggtc
     1321 gaaatcgtcg ggacgggagc tggcgtggtg gctgcgggaa tcgctgacga gggggagccg
     1381 cccgaagaag cgggaacggc ggctggagcc gggccggttg gcggcgttgc gccgagaaag
     1441 tgttcggcac gctcgagcag gagttttgcg gtgatgggtt tggccagata atcatccatg
     1501 ccgacggcca gacagcggtc gcggtcggct ttcgatgcca gcgcggtcaa cccgatgatc
     1561 accggttgct tgatatcggt catccggcga atctgactcg tggccatgaa gccatccatt
     1621 tcaggcatct gcccatccat gaagatcaga tcgaatgcgg aggtgcgaac cgtgtcgacg
     1681 gcaattcgcc cgttctcggc ggtagcgacg gcatagccgg ccttctccag aatgcggctg
     1741 gcgaccagcg tgttgacggg gttatcttcg acaatcagaa ttcggccggc gcgagacgga
     1801 gtcggcgtgc ttgccggcgg gggagcaacc gcgggtgaaa tggcgagagc ggcggtgggc
     1861 gtcgccgaag tgtcaccggc gagttcctga agcagaaatg tccgacgggt gggcagtcgc
     1921 aagaggcgaa tgcgttgcag agatgtgggt ggctgcgggt ggtggccccc ttgagag
//
LOCUS       DAORHD010001016         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73449_length_1975_cov_3.931771, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001016 DAORHD010000000
VERSION     DAORHD010001016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73449_length_1975_cov_3.931771"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PKO06_18125"
     CDS             complement(<1..1076)
                     /locus_tag="PKO06_18125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71628.1"
                     /translation="MMQIRSFPLMALIVVSSLVLFPFSVYAETDGQGAVILDGDLTFR
                     GDHYDVSGDTGNAPYPFTGTHRYLDFNLELHRDASDYESWHLRLGGVANDSRYRSADR
                     GAELERLRFAWEKGDAGTPFRFEAGEIYGNFTNRTLQRALRGFLLDLQPVTHGRIRRS
                     IQLLWGLARGDWKDPFADRDQSLGLSLLEERREDVRLSLNWVGNRREATVRRPELYQQ
                     VFSIAFEKPLDVHRRHSLESEYAWFRGDHEGDGTPGSGSDKKDSGLFLQLRGQMNPFG
                     YTWRFEEYGRDFRPAGANITQNRRALEGYLSWQLEKIRTLSLRLQRYRNSWESPNPLD
                     SDIIGIGLNGQVSPHDKLYMSLNA"
     gene            complement(1101..1646)
                     /locus_tag="PKO06_18130"
     CDS             complement(1101..1646)
                     /locus_tag="PKO06_18130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71629.1"
                     /translation="MNFARTLVSSSIVLLLASTFSDLVAAEFRTINPIHVPERASVET
                     ASNTDPALLQARKRLESRTVDLARAALTRLLEAWQTGGQLNDLLSEKFYDRQRFLDVR
                     DRVSPADAKVRLLAIESIQVLARSFDPHPQKTGSWLLNSRVSIRARTQLEYSHPKGGY
                     QKLEGVNEFVVHVAQPLLPKL"
     gene            complement(1660..>1975)
                     /locus_tag="PKO06_18135"
     CDS             complement(1660..>1975)
                     /locus_tag="PKO06_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71630.1"
                     /translation="LHVTPTYNIGDGDPWGHFDRAQANSGSTNWKLSVAPEATNALFY
                     NTAQNISVFNVNTRVTLFVEQNVVGGGGAKIKIELTVTRNGNGTAASDYQLVVHSAEY
                     IN"
BASE COUNT          460 a          587 c          509 g          419 t
ORIGIN      
        1 tacgcattca gactcatata gagcttgtca tgtggcgaaa cctgcccatt gaggcctatt
       61 ccaatgatgt cggaatcgag cggattggga ctctcccagg agttccgata ccgttgcagg
      121 cgcagcgaca gggtacgtat cttttcgagc tgccaggaaa gatagccctc gagtgcccga
      181 cggttctggg taatgttcgc cccagccggt cggaaatccc ttccgtattc ttcgaagcgc
      241 caggtgtacc cgaacggatt catctggccc cgcaactgca gaaacaatcc gctatccttc
      301 ttatcagagc cgctaccagg agttccgtcg ccctcgtgat cgccgcgaaa ccaggcatac
      361 tcgctttcca gagagtggcg ccgatgcacg tccaggggct tctcgaaggc aatactgaat
      421 acctgctggt acaattccgg acgtcgaacc gtcgcctctc ggcggtttcc cacccagttc
      481 aacgagagcc gcacgtcctc ccgacgctcc tccagcaacg acaggccaag gctttggtca
      541 cgatcggcaa aagggtcttt ccagtccccg cgcgccaacc cccagaggag ctgaattgac
      601 ctgcgtattc ggccgtgcgt gaccggttgc agatcgagca agaacccacg caatgcccgc
      661 tgaagcgttc gattggtgaa attcccgtag atctcacccg cttcgaatct gaacggcgtc
      721 ccagcgtctc ccttctccca ggcgaaacgc aaacgctcca gttcagcgcc gcgatcggca
      781 gaccggtagc gcgaatcgtt ggcgacacca cccaagcgca ggtgccagct ttcataatca
      841 ctggcatcac gatgtaattc gagattgaag tcaagataac ggtgagtacc agtaaaagga
      901 tacggcgcat tgcccgtatc accagagaca tcgtaatggt caccgcgaaa cgtcaagtct
      961 ccgtccagga tgaccgcgcc ctgaccatcc gtttccgcat acacggaaaa tgggaacaac
     1021 accagactgc tcacgacaat caacgccatg agaggaaaac ttcgtatctg catcatcgtt
     1081 gtcacctcgt tcggagaccg ttacagcttg ggaagaaggg gttgcgcaac atgcaccaca
     1141 aactcattca caccctccag cttctgataa ccacccttgg gatgcgaata ctcaagttgc
     1201 gtgcgggcac gaatcgatac ccggctattg agcagccacg acccggtttt ttgaggatgc
     1261 ggatcgaagg atcgtgcaag cacctgaatt gactcgatcg ccaacagtcg cactttggca
     1321 tcagccggac tgacccgatc gcgcacgtcg agaaagcgct gtcgatcgta gaacttctcg
     1381 gacagcaagt cgttcaattg ccccccggtc tgccaggcct cgagtagacg cgtcaacgcg
     1441 gctcgagcga gatcaaccgt acgagactcc aggcgttttc gcgcctggag caacgctgga
     1501 tccgtgttcg aagcagtctc gacggatgca cgctccggca catgaatcgg attaatggtg
     1561 cgaaactccg ctgcgaccag gtcggaaaaa gtactagcca gaagaagaac gatacttgag
     1621 gagacaagcg ttcgagcgaa attcatcttc gttctctcct tagttgatat actcagcaga
     1681 atggacaacg agttgatagt cactcgccgc cgtaccattg ccgtttctgg ttaccgttaa
     1741 ctcgatcttg attttcgccc cgcccccccc cacgacattc tgttcaacga atagtgtgac
     1801 tcgggtattg acgttgaaga cgctgatgtt ctgtgccgtg ttgtagaaca aggcattcgt
     1861 ggcctcgggt gccacactca gcttccagtt tgtacttccg gaatttgcct gagcacggtc
     1921 aaagtgcccc cacggatctc cgtcaccaat gttgtaggtg ggtgtcacat gtaag
//
LOCUS       DAORHD010001017         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73456_length_1975_cov_3.454167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001017 DAORHD010000000
VERSION     DAORHD010001017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73456_length_1975_cov_3.454167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="PKO06_18140"
     CDS             complement(<1..697)
                     /locus_tag="PKO06_18140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV71631.1"
                     /translation="MANGTASGRSHREMSDDTRQTAVRLERTKAARPVHLAIVAPCFN
                     EEQILADSANELMAVVERLKKSGKITPESGIYFVDDGSTDQTWSIIERLHRESPAIRG
                     IRLSRNFGHQGALLAGMMTLREKVDAVVTIDVDLQDEIGVLEQFVDLYRSGHDIVYGV
                     RRKRDTDSLFKRQTALTFYTLLEWMGVEVVYNAGDYRLIGSRPLAQLSEYSEVNLFLR
                     GIFPTMGFKAAQVE"
     gene            946..>1975
                     /locus_tag="PKO06_18145"
     CDS             946..>1975
                     /locus_tag="PKO06_18145"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HNV71632.1"
                     /translation="MSTLRTSVFCLLSLLAFSAIGSAADVHAPFAHLTGAGSGFVCTI
                     NAQTQGNQLNPGQALIFDGRADHAGYLNIVSLSQTGQLVRLFPNTFDPLGKLDGDKSF
                     RIPGDDRYAFRMGNIEETDQIKVFFTRQPLNAFPVTLWKKAGHFQLAKNTAEALQLLK
                     VNLDAAADQAVVISFTVRTRPGSASPDHISSLPASSGLQVSPTVSVSRIPESALQTES
                     ATPTASTSSSHASATTDAETEIEEDRIDPALFAVLSQPEPATPPIELPDVDLSVTATP
                     SHLPLAQTFTLDLNKPCERAVWSMRGVPWKTDPKDLQELTGAEEEAANPEPDYASFSF
                     DQSVYLRLV"
BASE COUNT          423 a          602 c          520 g          430 t
ORIGIN      
        1 attcgacctg cgccgccttg aaccccatgg ttgggaagat gccgcgcagg aagaggttca
       61 cttccgagta ttcggacaac tgcgccagcg gccgcgaacc gataagtcga taatcgccgg
      121 cgttatagac gacctcgaca cccatccatt ccagcagggt atagaaagtc agagccgtct
      181 ggcgtttgaa aagactatcg gtatcgcgtt ttcggcgtac gccgtagacg atgtcgtgac
      241 ccgaccggta caaatcgaca aactgttcca gcacgccgat ttcatcctgc agatcgacat
      301 caatagtcac gacggcatca actttctctc gcagggtcat catacccgcg agaagcgctc
      361 cctggtgacc gaagttgcgg gacaggcgaa ttccgcgtat ggccggtgac tcacggtgca
      421 agcgctcaat gatcgaccac gtctgatctg tgctgccatc gtcaacaaag tagattccgc
      481 tctctggagt gatcttcccg gacttcttca gtcgttccac cacagccatc aactcgttcg
      541 cactgtcagc cagaatctgt tcttcgttga aacagggggc aacgatcgcg aggtgcaccg
      601 gacgcgcagc cttggttcgc tccagccgga cggcagtttg acgcgtatca tccgacatct
      661 cgcggtggct tctccccgag gcggtaccat tggccatctt ccgctctcct ttcgttgcct
      721 gcgaggctca tcagagccgg tgtcgccgca ctcaccgagg ttcttccctg acgcacgcac
      781 gggtgagcac gtctcgggaa aagggctctc aaacgggagc catcatagta gtttgcctcg
      841 tctaaggtca acccaggttc ggggcaaatg cttctctcgt atgttggtcg gtcgggcata
      901 tggtaaagtg cagaaaactg gatcttcagg aggatgtgcg cgccaatgtc gactcttcgc
      961 acctcggtgt tctgtctcct ctctctcctg gctttcagcg caatcggctc tgccgctgat
     1021 gttcatgctc catttgccca cctgacaggt gcggggtcgg gttttgtctg cacgatcaat
     1081 gcgcagacac aaggcaatca actcaacccc ggacaggcac tcattttcga cggacgcgcc
     1141 gatcacgcgg ggtatctcaa cattgtgtca ctgtctcaaa ccggtcagct ggtacgattg
     1201 tttcccaata ccttcgaccc cctggggaaa ctcgacggcg acaagtcttt tcgcatccca
     1261 ggcgatgatc gctatgcgtt tcggatgggg aacatcgaag aaacagacca gataaaggtt
     1321 ttctttaccc gacaaccact caatgctttt ccggtgacgc tgtggaaaaa ggcaggtcat
     1381 ttccagttgg cgaagaacac ggcagaagca ctgcagttac tcaaggtcaa cctggacgca
     1441 gccgctgacc aggctgtggt catttctttc accgtcagaa cgcgtcctgg ctcggcttcg
     1501 cctgatcata tcagcagtct gccagcctcg agtgggctgc aggtcagccc gacggtttcc
     1561 gtgagccgaa ttccggaatc ggcactacag accgaatctg cgaccccgac agcatccacc
     1621 tcgtcatctc acgcatctgc aaccactgat gcggaaaccg agatcgaaga agatcgtatc
     1681 gacccggcct tgttcgcggt tctctcacag ccagagccgg cgacgccgcc aatcgaactg
     1741 cccgatgttg atctctccgt gacggcaacc ccctcgcacc ttccgcttgc ccagacgttc
     1801 acactcgacc tgaataaacc gtgcgaacgt gccgtgtgga gcatgagagg cgtgccgtgg
     1861 aaaaccgacc cgaaagacct gcaggaactt accggggccg aagaagaagc ggcaaacccc
     1921 gagcctgact atgccagctt ttcctttgat cagtcggttt acctgcgtct cgtgc
//
LOCUS       DAORHD010001018         1975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73491_length_1975_cov_2.033854, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001018 DAORHD010000000
VERSION     DAORHD010001018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73491_length_1975_cov_2.033854"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKO06_18150"
     CDS             <1..473
                     /locus_tag="PKO06_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71633.1"
                     /translation="YNPGHGGSNGGYNPGHGGSNGGYTPGHGGNNGGYNPGPGGHHPG
                     PGHDQISFALREAHRDAKRLFSFVINSFHPGMGEMEKRQTARYMRDLSRALITVAEEA
                     PRPFRLELREVYQMVEDARFILMTEDSARVARGKLERAERMYMEAVRRFMGWNA"
     gene            complement(523..1110)
                     /locus_tag="PKO06_18155"
     CDS             complement(523..1110)
                     /locus_tag="PKO06_18155"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV71634.1"
                     /translation="MEREDEYDDSAIHQELSTSHHDPLIRILHHAIRVCIKLLAVLMV
                     FVIFLGVLVWAGYTILLKRSLQTHTALEATGYSLILGAVLFLPIGVPAMASQDFHRIS
                     LAAWGSVAYLSLLTSVLAYFVWSWALSHVEAGRIAIVSNLQPVVATALAWYFLQEPIT
                     AQFLVGTSLTITGVILTQLHPLASSIPTAEAHHQT"
     gene            complement(1223..1690)
                     /locus_tag="PKO06_18160"
     CDS             complement(1223..1690)
                     /locus_tag="PKO06_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008294858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNV71635.1"
                     /translation="MSEIDATTPVIDPAEIRLAAMNLLARREHSVWELRRKLRTRFPD
                     EALLDEQLSRLAEQNLQSDMRFAESYIRQRIYRGYGPVRLREELRERGISRTDIAQLM
                     DELAIDWRAHALQVMQNKFGGLPPRDLKEEARRARFMQHRGFTAEHYKHSRED"
     gene            complement(1813..>1975)
                     /locus_tag="PKO06_18165"
     CDS             complement(1813..>1975)
                     /locus_tag="PKO06_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNV71636.1"
                     /translation="KIGQGKANAAKFLGSNPALSNEIEGQIRAQMLNIDAPIKVPAAA
                     QDEESLSEE"
BASE COUNT          438 a          525 c          597 g          415 t
ORIGIN      
        1 gctacaaccc tggtcacggc ggcagcaacg gtggctacaa ccctggtcac ggcggcagca
       61 acggtggcta cacccccggt cacggcggca acaacggtgg ctataacccc ggtcctggtg
      121 gacatcatcc tggtcccggg cacgatcaga tcagtttcgc gcttcgtgaa gcgcatcgcg
      181 atgccaaacg tctcttctct ttcgtcatca actcatttca ccctggaatg ggcgagatgg
      241 aaaagcgtca gacggctcgc tacatgcgtg atctgagtcg ggcactcatc acggtggctg
      301 aagaggctcc gcgccccttc cgtctggaac ttcgcgaagt gtatcagatg gtcgaagacg
      361 cccgtttcat cctgatgacg gaagacagcg cccgcgttgc tcgcggcaag ctcgaacggg
      421 ctgagcgcat gtatatggaa gccgtgcgac ggttcatggg ttggaacgcc tgatcagcaa
      481 agcaatttga gaaagcggcg ccggtacatt ccggcgccgt ttttatgtct ggtgatgagc
      541 ttcggctgtc ggaatactgc tggcgagtgg atgcagctgg gtgagaatga ccccggtaat
      601 ggtgagactg gtgccgacca gaaactgagc ggtgatcggt tcttgcagaa aataccaggc
      661 cagcgcggtt gcgactacgg gttgcagatt tgagacgatg gcaatacgac cggcttcgac
      721 gtgactgagg gcccacgacc agacgaagta cgcgagcacg gaagtcagaa gcgaaaggta
      781 ggctactgaa ccccaggcgg cgagagagat acggtgaaag tcctgtgaag ccattgcggg
      841 aacgccgatg ggaagaaaga gaacagcccc aagaatgaga gaataccccg tggcttcgag
      901 tgcggtgtgg gtttgcaacg atcgtttgag gagaatggta taaccagccc agaccagcac
      961 gccaagaaaa atcacaaaga ccatcaacac cgccagtagc ttgatgcaaa ccctgatggc
     1021 gtgatgcaga atgcgaatga gtggatcgtg gtggctcgtc gataattcct ggtgaatcgc
     1081 agaatcgtcg tactcgtctt cccgttccat gcttcgaatt ccccgaattg cgccggcgtc
     1141 tgccgccagg ttgaatcacg gaaaatactg ttatttcaac gctcgagcaa ggccccgtga
     1201 cgaattagca gtgcaggtgg aattagtcct ccctcgaatg cttgtagtgc tcggccgtga
     1261 atccgcggtg ctgcataaat cgggcgcgac gcgcttcttc cttgagatcg cgtgggggta
     1321 aaccgccgaa cttattctgc atcacctgca gcgcatgcgc gcgccaatca atcgcaagct
     1381 cgtccatgag ctgcgcgatg tctgtgcggg agattccgcg ttcgcgcaat tcctcgcgca
     1441 agcgcacagg cccgtaacca cgatagatac gctggcggat gtaactctcg gcgaagcgca
     1501 tgtcgctctg gagattctgc tcggcgagtc gggatagttg ttcatccagc agggcttcat
     1561 cgggaaaacg ggttctgagt ttcctgcgca attcccacac ggagtgttct cgacgcgcaa
     1621 gcaggttcat cgcggcaagg cgtatctcgg ccgggtcgat gacaggggtt gtcgcgtcta
     1681 tctcggacat gaactcaggc tcggacatga acccagacag gagaaaaaaa ggcgtcgggt
     1741 acgtgaatgg ggtacgcggg gtcccgacgc ctgtggaaat ggttcatcga acgtattgtc
     1801 tggcggagtg tattactcct cggacagact ctcctcatcc tgagcagctg cgggaacctt
     1861 gatcggcgcg tcgatgttta acatttgcgc gcggatttgc ccttcaattt cattggataa
     1921 cgcgggattg cttcccagga acttggcggc attggcttta ccctggccta ttttg
//
LOCUS       DAORHD010001019         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73524_length_1974_cov_3.158937, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001019 DAORHD010000000
VERSION     DAORHD010001019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73524_length_1974_cov_3.158937"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PKO06_18170"
     CDS             <1..854
                     /locus_tag="PKO06_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71637.1"
                     /translation="QITLLIFALVLCGNLVFAAGSFLETVSEADKAGVGGESRLSDTD
                     RATSAARNRLNTWREQQSYHLDLTKLMNSVYTAVTKPTVSWTTAVQLPPTPASLATAS
                     LQLLAQTIFKYFGISLTSTDATQWYSSTAALANWTQQQALRLYELLYKLPKFFTQATK
                     KLARVVSYKGTTNVLGYVYSFIPTAHICNSAVRSVALFQETIVHEMTHCFQFSNMTVM
                     NQWASSFWKNNKPIKSSPTSYGNTNAYEDFAESVRLYVAYGKQLKSYDSTRYEFIKKY
                     VMQGKEF"
     gene            1131..>1974
                     /locus_tag="PKO06_18175"
     CDS             1131..>1974
                     /locus_tag="PKO06_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71638.1"
                     /translation="MRQKKSLWFRLSSILLALMATQFLTGCAGGGGSGVNTPVGPSPD
                     GKTAIDGYVSYNAASPLVRAKVAGAGFQPLKGAVVELVAFDETGAEHVLGKTSTDANG
                     YYRLAEVVNPYGWRNLIIRALAAGFELEGVLPQFLPGSIQHAQTIDATTGVGARIVRQ
                     AAKLGQVTKVNLGELYSLLPESSLRRLSDTALGTIVAGVVAREQVRKQKLGSLADQLM
                     AYAFDLHTQLQAMIEAGDMTAAEAWEAFARKMALKADELGVTPEQMHALDDADQALIY
                     EPGLV"
BASE COUNT          411 a          557 c          570 g          436 t
ORIGIN      
        1 ggcagattac actactgatc ttcgctctgg tgctctgtgg caatctggtg tttgcggccg
       61 gcagcttcct cgagacggtt tctgaagcgg acaaggcggg cgtggggggc gaatcgcggc
      121 tttctgatac cgatcgcgcg acgagtgcag cacgaaaccg gttgaatacc tggcgtgagc
      181 aacagtcgta tcacctcgat ctgaccaagc tcatgaactc agtgtatacg gccgtgacga
      241 agccgaccgt gagctggacg actgccgtac aactgccgcc gacccctgct tccctggcga
      301 ccgcttcgct gcagctgctg gcgcagacga tcttcaaata cttcggcatt tcgctgacca
      361 gcaccgatgc aacccagtgg tacagctcga ccgcagccct cgccaattgg actcagcagc
      421 aggcactgcg attgtacgaa ctgctgtaca aactgccgaa atttttcacg caggcgacca
      481 aaaaactggc acgtgttgtt tcctataagg gaaccacgaa cgtgctcggg tacgtgtact
      541 cgtttattcc gaccgcccat atctgcaaca gcgcggttcg ctccgttgcc ctgttccagg
      601 aaacgatcgt gcacgagatg acccattgct ttcagttcag caacatgacc gtcatgaacc
      661 agtgggcctc cagcttctgg aagaacaaca agccgatcaa aagctcgccg accagttacg
      721 gcaataccaa cgcctacgaa gacttcgccg aatcggtgcg tctctacgtt gcctacggca
      781 aacaactcaa gtcctacgac agcacccgct acgagttcat caagaaatac gtcatgcagg
      841 gaaaagagtt ctaagcctcg ctgttcgtcg ttcgttccgg cctgaggaaa acccaagccc
      901 ggttgtgagt cgtgagagag ctcctctcga cccgctccgg gcttcttcat ttcttcggtc
      961 ggcacccagg ttgatcattc ggtttccggt cgaaagatct tgtcttgcct tctcagcctg
     1021 catctcgaag agatttggag cattccgccc ctggtcgtga tctgtatctt cagagtcaga
     1081 gggataaatg ccgatatgag gttggatagg ttttatctga ggaggcaggc atgagacaaa
     1141 aaaagagttt gtggtttcgg ttgagttcga ttctgctggc cctgatggcg acccagtttt
     1201 taaccgggtg tgcgggtggt ggtggctccg gtgtgaatac gccggttggc ccctcgcctg
     1261 acgggaagac ggccatcgat ggctatgtca gctataatgc cgcgtcaccg ctggtgcgcg
     1321 cgaaagtggc tggagccgga tttcagcctt tgaagggcgc ggtggtcgaa ctggtggctt
     1381 tcgacgagac tggtgccgag catgtgctgg gcaagacctc gaccgacgcg aatggctact
     1441 accggctcgc cgaagtggtg aatccgtacg gctggcgaaa cctgatcatt cgggctctgg
     1501 ccgccggttt cgaactcgag ggggttctgc cgcagttcct gcccggttcg attcagcatg
     1561 cccagaccat cgacgccacc acgggtgtgg gtgcgcgcat tgttcgacag gcagcaaagc
     1621 ttggacaggt cacaaaagta aatctcggcg agttgtactc tttgttgccc gagtcgtctt
     1681 tgcgtcgact ttctgatacc gctcttggca cgatcgtggc tggagttgtg gcacgcgaac
     1741 aggtacgcaa gcagaaactc ggctcgcttg ccgaccagtt gatggcctat gcctttgatc
     1801 tgcacacgca gctgcaagcc atgatcgagg ccggtgacat gaccgccgcc gaagcctggg
     1861 aagcattcgc gcgcaagatg gcgctgaaag ccgacgagct tggggttacc cccgaacaga
     1921 tgcatgcact cgatgatgct gatcaggcgc tgatctacga gcccggcctc gtcg
//
LOCUS       DAORHD010001020         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73768_length_1970_cov_3.107572, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001020 DAORHD010000000
VERSION     DAORHD010001020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73768_length_1970_cov_3.107572"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            756..>1970
                     /locus_tag="PKO06_18180"
     CDS             756..>1970
                     /locus_tag="PKO06_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71639.1"
                     /translation="MFRNSLVLCQARRLSLYLRPFAGRLLLAAATMLLVTGLHLLRPL
                     ILRELIDRALPQGNTTLALHLGLAFVGALAVGALALYGRAVLLAQVGTGCVARLKADL
                     LERLLSREMAFFHRHAPGKLISRVENDIEQMRGLVSGASAQCLANVLLLVAISSLMIW
                     QEPKLVGWLALAFLGGGSAVFLYARHVRDLYREVRERDSELSARLTEFLQGVSLLRLY
                     GREEAAVQRISDATRHRCEIERRAGFYDTVLFYGGFTLIAEIGSLAVLLWYGSTEVFA
                     GRMTVGTLVMFLELMRRFFGPLRELAEVLIQMQSGLAATGRVLTLLEQVGETTSSEVS
                     AVEAPVRGGPGLERVPAALVLALDPGGLLPDCCWVAGISDTTMPRVDPVSHWQEGGEK
                     GLEALPGTGFHEV"
BASE COUNT          347 a          549 c          645 g          429 t
ORIGIN      
        1 tgcttttcgg cccgcgagac ccagaaaccg acgagcggtg aaagatcgga aaggtcggcc
       61 gccttgggca gagggtcggc aaactctggg aacgtgcagc ccgaaaggca gaagagcata
      121 gtcaacgcca ggcaatacct gagcgggaca cgcgagagaa cgcacaacat ccatgacttc
      181 ataccagaga gaataccatg gttctgttcc ccctgctcga gagtggtcga gccagtgctc
      241 ttctataaca acgcgaaatg agtcctagtt gtgaaccaaa atatcagtga aatagcgctt
      301 gacagccaac gtggatttga gtatattcgt cacacctgat caacaggctc attttcaccg
      361 caaaggagga ccgcacatga taacgatttc ccgtggcaag aacccgtgcg ccgtatcctc
      421 cttgaggcac tcctgatcag gtttttctcg tctccctttt gaaccactga cagggccgcc
      481 gaaaggcggc cctgttgctt ggcgcggcga aatcgcgccc gattcgtgtt tttcccctga
      541 ccctcattca acgtcacggc atcaagccgg aaggagaacc ttcatgtact cacctcgact
      601 gtttctgcgc ccccgctcgc ggggctgtgc aaagtggctc gcattctcgg ccgggagaaa
      661 agtcctggtc gctggatggc tggagtggcc agataagctg atttccgcga tggagtattc
      721 catctctggt tctggactac gtaaaggaag aaggtatgtt ccgcaactct cttgttctgt
      781 gccaggcgcg cagattgtct ctgtatctgc ggccgtttgc cggccgcctg ttgctcgccg
      841 cggcgacgat gctgctggtg accggtctgc atctgctgcg gccgctgatt ctgcgtgaac
      901 tgatcgatcg ggctctgccg cagggcaaca cgactctggc cctgcatctg gggcttgctt
      961 tcgttggggc tctggctgtc ggagcgctgg ctttgtacgg gcgagcggtt ctgctcgccc
     1021 aggtgggaac gggttgtgtg gcccgtctga aagcagatct gctggagcgt ctgctctcgc
     1081 gagagatggc tttctttcac cgccatgcgc cgggcaagct gatcagtcgc gtcgagaacg
     1141 acatcgagca gatgcgcggg ttggtctcgg gagcgagcgc gcaatgtctg gcgaacgtgc
     1201 tgctgctggt ggcgatctct tcgctgatga tttggcagga accgaagctg gtcggctggc
     1261 tggctctggc ttttctcggc ggcggaagcg cagtgttcct gtatgcccgc catgttcgcg
     1321 atctctatcg cgaggtgcgt gagcgtgatt ccgaactgtc ggcccgtttg accgagtttc
     1381 tgcagggcgt gtcgctgctg cgtctctatg ggcgcgagga agcggcggtg cagcgcatca
     1441 gtgacgccac gcgtcatcga tgcgagatcg agcgccgggc gggtttctat gacactgtgc
     1501 tgttctatgg cggcttcacg ctgatcgccg aaatcggcag tctggccgtg ctgctctggt
     1561 atggcagcac cgaagtgttt gccggtcgga tgacggtcgg cacgttggtg atgtttctcg
     1621 aactgatgcg gcgcttcttc gggccgttgc gagaactggc cgaagtgctg attcagatgc
     1681 agtcggggct tgccgccacg gggcgggtgc tgacgctgct cgaacaggtc ggagagacga
     1741 cgtcgtcgga ggtcagcgcc gtcgaggcgc cggtgagagg cgggcccggg ctcgaacgtg
     1801 tgccggccgc tctggttctg gcgctagacc cgggtggcct gctgccagac tgctgctggg
     1861 ttgccggaat cagcgacacc acgatgcccc gcgtcgatcc ggtgagccac tggcaggagg
     1921 gcggcgagaa gggcctagaa gcgctgccgg ggactgggtt tcacgaagtc
//
LOCUS       DAORHD010001021         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73835_length_1969_cov_3.056426, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001021 DAORHD010000000
VERSION     DAORHD010001021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73835_length_1969_cov_3.056426"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PKO06_18185"
     CDS             complement(<1..658)
                     /locus_tag="PKO06_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71640.1"
                     /translation="MNWRRWVGSVLVAAGLLLPVPSPAWDCNTTHPSINELAAKLFIV
                     KSQSETFGEKYRSAPIEFRTVFKGTALKKGTFSENASVKETITANFIDWVARGGHDAD
                     VPGFVMAFRHFYDPVYEPRHLTWFRRWLNKPDKEDFASEEAFFNSYKYKANEWNINKP
                     EGDKPAKLLFQPKVIKPRIDGITWAVSHPDNDHSWHNGLRAYKAALENDQTALGKLNR
                     E"
     gene            862..1692
                     /locus_tag="PKO06_18190"
     CDS             862..1692
                     /locus_tag="PKO06_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV71641.1"
                     /translation="MLDFSIDQGRCTGCGACVHDCPARVLELVEGKAGMVAGKAEECI
                     KCQHCLAICPVEAVTIGGVHASDCLKLDGQFPTPEQLELLMRGRRSVRAYKRENLPPE
                     LIQRLVEVAWQAPTGVNDRQVLFTVIDDLQTMDRMRQELMGELAKVVRGGTLPERLAM
                     YAKFVELWETKKIDILFRGAPHLIIASAPKDSPCPHEDCLIALSYFELYAHCQNVGTV
                     WDGLAKWAFEALIPGFPHRLGVPKDHKLGYVMAFGRPAVRYPRTGKHAPVLLNRVRTL
                     "
     gene            complement(1702..>1969)
                     /locus_tag="PKO06_18195"
     CDS             complement(1702..>1969)
                     /locus_tag="PKO06_18195"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV71642.1"
                     /translation="QQEAVITSFFALFPSILLSGLIFPISNMPYPVQIITYAIPLRYF
                     LEITRSIFLKGVGLEYLWPQTLVLIVMGWAIITLAVNRFSKRLS"
BASE COUNT          414 a          510 c          613 g          432 t
ORIGIN      
        1 gttcgcgatt cagctttccg agtgccgtct gatcgttttc aagggcggct ttgtaggctc
       61 tgagcccgtt gtgccacgag tgatcgttgt cggggtgact gacggcccag gtaatgccgt
      121 cgatgcgagg cttgatcact ttaggctgga acagcagttt cgcgggtttg tcgccctcgg
      181 gcttgttgat attccactcg ttggctttgt atttgtacga gttgaaaaaa gcttcttcac
      241 tggcgaaatc ttctttgtcg ggcttgttca gccagcgtcg aaaccaggtc aggtgacgag
      301 gttcatagac cggatcgtaa aagtgccgaa aggccatgac gaacccggga acatcggcgt
      361 cgtgaccgcc gcgcgcgacc cagtcgatga agttggcggt gatcgtttcc ttgacactgg
      421 cgttctcaga gaaggtgccc ttcttcaggg cggtgccctt gaagacggtg cgaaactcaa
      481 tcggagccga gcgatacttc tcgccaaacg tctcggactg actcttcacg atgaacaact
      541 tggccgccag ttcattgatg gaaggatgcg ttgtattgca gtcccatgca ggcgatggca
      601 ccggcagcag taaacccgcc gccaccagca cggaaccgac ccagcgtcgc caattcatgc
      661 tcgatacccc cgtcgcagca ccagaaaacc gctgccgttt ctctcattat acgaatgaaa
      721 cccacaaccg acaagacttg taatgaaact ggatggtatc atatatggct gaagccgtcg
      781 gcttgattgg cggcggagaa cgcgctacag tgccgaacag gcagtactgg ctggcagaat
      841 atcgttgaaa ggggacacgg aatgttggac ttttccattg atcaggggcg gtgtaccggg
      901 tgcggcgcgt gcgttcatga ttgtccggcg cgggtgctgg agctggttga aggcaaggcc
      961 ggcatggtcg ccgggaaggc ggaagagtgc atcaaatgtc agcactgtct ggctatctgt
     1021 ccggtcgagg ctgtcacgat cgggggtgtt catgccagcg actgtctgaa gctcgatggc
     1081 cagttcccaa cgccggaaca actggagctg ctgatgcgag gacgccgttc cgtgcgggcc
     1141 tacaagcgcg aaaatcttcc gcctgaactg attcagcgtt tggtggaggt tgcctggcag
     1201 gcaccgacag gggtgaatga ccgtcaggtg ttgtttaccg tgatcgacga cctgcagacc
     1261 atggatcgca tgcgccagga gttgatggga gagctggcca aggtggtacg tggtggcacg
     1321 cttccggaac ggctggcgat gtatgcgaag ttcgtcgagc tgtgggaaac caagaagatc
     1381 gatattctgt tccgcggcgc gccgcatctg atcatcgctt ctgcgccgaa agacagcccc
     1441 tgtccacacg aagactgtct gatcgcgctg tcatatttcg aattgtatgc gcattgtcag
     1501 aacgtcggca cggtctggga tggcctggca aagtgggcat tcgaagcctt gattccgggg
     1561 tttccgcacc gcctgggcgt gccgaaggat cacaaactcg gctatgtgat ggcgttcggg
     1621 cggcccgcgg ttcggtatcc gcgtaccgga aaacatgcgc cggttctgct gaatcgcgtg
     1681 cgcaccctct gacggcgcgg gttaactcaa ccttttactg aagcggttta ccgccagagt
     1741 gatgatggcc caccccatga cgatcagcac gagcgtctga ggccacagat actcgagtcc
     1801 gacccctttc agaaaaatcg atcgggtgat ttcgagaaag taccggagcg gaatcgcgta
     1861 ggtgatgatt tggacggggt atggcatgtt gctgatcgga aagatcagcc ctgacagcag
     1921 aatcgaggga aacagggcga agaagctggt aatgacggcc tcctgctgt
//
LOCUS       DAORHD010001022         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73950_length_1967_cov_2.789226, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001022 DAORHD010000000
VERSION     DAORHD010001022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73950_length_1967_cov_2.789226"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1073)
                     /locus_tag="PKO06_18200"
     CDS             complement(63..1073)
                     /locus_tag="PKO06_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013751434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV71643.1"
                     /translation="MGVPVLVTGGAGFIGSHVARHLIANGCEVVVLDDCSGGFRENVP
                     EGARFVVGDAGDPDLIETLFRAHAFQQIFHLAAYAAEGLSHFIRRYNYTNNLLVSTTL
                     LNAAVRHKVKTFVFTSSIAVYGRNQLPMTEVLEPRPEDPYGIAKYAVELDLRAAHEMF
                     GLDWIVFRPHNVYGTNQNLGDPYRNVIGIFMNQIMQGKPLTIFGDGSQTRAFSHIDDV
                     APVIARSIDVPGARNQVFNIGADAPVSVNDLARAVMKAMGREVAIDHLPARNEVIHAF
                     ADHGKVRQVFGLTNAPISVEDGLHEMADWAWKAGPRQGKPFTGIEIEDGLPPSWKKLT
                     IR"
     gene            complement(1240..1893)
                     /locus_tag="PKO06_18205"
     CDS             complement(1240..1893)
                     /locus_tag="PKO06_18205"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71644.1"
                     /translation="MKVHVWMLSVLVGMLLCFGPVWAQPVGPEGPDAEIDGPPMGPGG
                     QMGPGGPGMGPGGPGMGHGGPGMGQGGPGMRGQHGKPSPEQLAKMKKMQAMRSTAEAY
                     KNLAELYKEQGKIDEAVAQLKKILALANDAEAKDDPRVKNQLGRVYLEIAELYVSKDR
                     LADAEAIINEGVEKTKGDNPEVSSRMLLTLGNIYRKKGKTAEAEKVFQRVIELNTGK"
BASE COUNT          398 a          592 c          550 g          427 t
ORIGIN      
        1 gccgatgatc aagcgtcact cgcgtttccg gtcgctgatt tcttgcgatc gagcccgaag
       61 gttcaccgaa tggtcagttt tttccacgac ggcggcagac catcttcgat ctcgatcccg
      121 gtaaagggtt ttccctgccg gggtccggct ttccaggccc agtcggccat ttcgtgcagc
      181 ccgtcttcaa cggagatcgg agcgttcgtc agaccgaaca cctggcggac ttttccgtgg
      241 tcggcaaagg cgtggataac ttcgttacgg gcgggaagat gatcgatcgc cacttcacgg
      301 cccatggctt tcatgactgc gcgcgccaga tcattgaccg aaacgggcgc atcggctccg
      361 atgttgaaga cctgattgcg agccccgggc acatcgatcg aacgcgcgat cacgggggcg
      421 acatcatcaa tgtgactgaa ggcacgcgtc tgactgccgt ctccgaagat ggtgagcggc
      481 tttccctgca tgatctgatt catgaagatg ccgatcacgt tgcgatatgg atcgccgagg
      541 ttctgattgg ttccgtagac attatggggg cgaaacacga tccagtcgag gccgaacatc
      601 tcatgcgcgg cccgcagatc gagttcgacc gcatatttgg cgatgccata ggggtcttcc
      661 gggcgaggct cgagcacttc ggtcattggt agctggttgc ggccgtacac ggcgatcgaa
      721 ctggtgaaga cgaaggtctt caccttgtga cgcacggcgg cgttcagcag ggtggtgctc
      781 accagaaggt tgttggtgta attgtaccgg cgaataaagt gcgaaagccc ttcagcagca
      841 tatgcggcaa gatggaagat ctgttggaac gcatgggcgc gaaacagggt ctcgatgaga
      901 tcgggatcac ccgcatcacc caccacgaaa cgagcacctt cgggaacatt ctcgcgaaag
      961 cccccggagc agtcgtcgag cacgacgact tcgcacccgt tcgcaatcag atgccgtgcc
     1021 acgtgtgagc cgatgaatcc agcaccgcct gtaaccagaa ccggtactcc catacaacct
     1081 cgcttttctg agccgattga tccacggccg aagatttcta ccgggaaata ctagcatggg
     1141 cagcgaagaa agaaaacgag cccgggggaa aagaaaacgg acccggcggc gggggccgac
     1201 gagtccgagt tatgaacaac cgcgaacgtc tgatgatgat tactttccgg tgttcagttc
     1261 gatgacacgc tgaaacacct tctctgcttc ggcggtcttg cccttcttgc gatagatgtt
     1321 gcccagggtc aacaacatgc gtgaagacac ttccggattg tcgcccttgg tcttttcgac
     1381 cccttcgtta atgatcgctt cggcatcggc caggcgatct ttcgaaacgt acaactcggc
     1441 gatctcgaga tacacgcgtc caagctgatt cttgacccgt ggatcatctt tggcttcggc
     1501 atcatttgcc agcgccagaa tcttcttgag ctgcgccacg gcctcgtcga tctttccctg
     1561 ctccttgtac aactcggcca ggttcttgta ggcttcggct gtcgagcgca tcgcctgcat
     1621 ctttttcatc ttcgccagct gttccggtga cggctttcca tgctggccgc gcatccccgg
     1681 tccgccctgc cccatcccag gaccgccatg gcccatcccc gggccgccgg gtcccattcc
     1741 tggaccaccg ggacccatct gtccgccagg gcccatcggg gggccgtcga tctcggcatc
     1801 ggggccttcg gggcccaccg gctgggccca caccggcccg aaacacagca acatgccaac
     1861 caacacagac agcatccata cgtgaacttt catgactgtt cctcctcgag aaatgtatag
     1921 gttgtttcgt cgtcgaggaa ttcaaactcc ccgacttgtt cccgttg
//
LOCUS       DAORHD010001023         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_73956_length_1967_cov_2.716004, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001023 DAORHD010000000
VERSION     DAORHD010001023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73956_length_1967_cov_2.716004"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKO06_18210"
     CDS             <1..724
                     /locus_tag="PKO06_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71645.1"
                     /translation="ECFAATLGLDVQRILAWSFAQAVLAAIWEIEDHGSLRHGIHWIM
                     LANTVLPMLDRQISTDSSVASTAGFRVWNIAIGGKSKEDLLSLLHKSDIQLNEYARVL
                     FSSDLFLTSTKRETRRIVELRVSDLQISGPATLRAIIQRARERDLEPCPLEVGPCFRL
                     QFTNQEEEREASSHKAPQGSITIISDLFELEDENFPRGFYLRKIQNKFWLRGYVCSED
                     YVWNNEDRIAFQICHRRKEPIG"
     gene            721..975
                     /locus_tag="PKO06_18215"
     CDS             721..975
                     /locus_tag="PKO06_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71646.1"
                     /translation="MKTFFPCPKCASEKIIPEIAIHDRDDCGGDTLGIRIDSSPEAWL
                     FKNPHIVELRATICCDCGYTEFFSSGDLNRLWNLHQQQHR"
     gene            980..1330
                     /locus_tag="PKO06_18220"
     CDS             980..1330
                     /locus_tag="PKO06_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71647.1"
                     /translation="MTDRTHPRMTFMQPDARADDTRQKCPFCGCGQPVSQRYPEYACW
                     DCSEKAVDENQRPLVFYNTSFGGGFEARFRDTDEVRAGNICYINGIECWADEARFGGI
                     VIQPRRKPDSDNHP"
     gene            complement(1565..1822)
                     /locus_tag="PKO06_18225"
                     /pseudo
     CDS             complement(1565..1822)
                     /locus_tag="PKO06_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          445 a          550 c          501 g          471 t
ORIGIN      
        1 cgaatgcttc gctgccacgc ttggcctcga tgtccagcgg atacttgcct ggtctttcgc
       61 ccaggccgtt cttgccgcca tctgggaaat cgaggaccac ggctctctca ggcacggtat
      121 tcattggatc atgctggcga acacggttct gcccatgctc gacagacaaa tttcaaccga
      181 ttcttccgtg gcttccaccg caggattccg cgtctggaac atcgccatcg gtggcaagtc
      241 gaaagaggac cttctcagtc tcctgcacaa atcagacatt cagttgaacg agtatgccag
      301 agtacttttt tccagcgacc tctttctcac aagcacgaaa agagaaactc ggcgcatcgt
      361 cgaactacgt gtctctgatc tacagatttc cggaccggca acactcaggg cgattattca
      421 gcgtgcacgc gaacgtgatc tcgaaccctg tcctcttgaa gttggtccgt gtttcaggct
      481 gcagttcacc aatcaagagg aagagcgcga agcgtcttca cacaaggctc cacaagggtc
      541 gatcaccatc atttcagatc ttttcgaact tgaggatgag aactttccca gagggtttta
      601 tctgcgaaag atacagaaca agttctggct tcgaggatac gtctgctctg aagattatgt
      661 ctggaacaac gaagaccgca tcgcgttcca gatctgtcac agaagaaagg aaccgatagg
      721 atgaagacgt ttttcccgtg tccgaagtgt gcatcagaga aaatcatacc ggaaatcgcc
      781 attcatgatc gcgatgactg tgggggcgat actctgggta ttcgcatcga ttcttcgccc
      841 gaagcctggc tgttcaagaa cccgcacatt gtcgaattac gcgcgaccat ctgctgcgac
      901 tgcggttaca cggaattttt cagttctggt gacctgaatc gtctctggaa tctgcatcag
      961 cagcagcacc gctgacgcca tgaccgaccg gactcaccca cgcatgacct tcatgcagcc
     1021 ggatgcgcgc gccgatgaca ccaggcagaa atgtccgttt tgcggatgcg gacaaccggt
     1081 cagccagagg tatccagagt atgcctgctg ggactgcagc gaaaaggccg tcgatgagaa
     1141 ccagcgccca ctggtgtttt acaacacctc attcggaggt ggattcgagg cacgatttcg
     1201 ggataccgat gaggtgcgtg ccggcaacat ctgttacatc aacggaatcg aatgctgggc
     1261 cgacgaagcc cgtttcggcg gcatcgtcat tcaaccacgc cgcaaacccg actccgacaa
     1321 tcatccttag cctctcaact gggacatcgg cgtggaatgc gtcgaaatga aaaacctgcc
     1381 cggcataaga gctcgggcag gtatgagatc ttgcgataga actcgtcact cgcgtgacga
     1441 aaggtacaag ttactttttc gctttgcctt ttgcgggtgc cttcttcgcg gcgggtttct
     1501 tcgcagtcga tgcttttgcc gctggcttgg ctttgggcgc tttctgttcg ctatcgatca
     1561 cctcgaggca gaccagttcg gcatgaacgc cgtgaaccag gttcagcttg cgggcgattt
     1621 cgaggtgctt cctgttgtcg ccgacgagct cgaggatcag cagaccagca tcggaacaat
     1681 gatcgagcgt gccttcgtgc agacccaggc gggtcttgat gaagcaaccc cagccggtca
     1741 gaatcttctg caccatgacg gcagattcct tgcggctgcc gatgagaatc atcagaactg
     1801 ttcgttcagt cttcttcgcc attctttcac attcctccac gtaagatggt tgttggtaca
     1861 ttcactgtag catagatcgt ctcgaagtca acacaccaac cagggtcatt tcattctcct
     1921 ccttacttgt tttccgttca catcgctcaa cgattcaccg ttacggc
//
LOCUS       DAORHD010001024         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74219_length_1962_cov_3.488726, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001024 DAORHD010000000
VERSION     DAORHD010001024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74219_length_1962_cov_3.488726"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /locus_tag="PKO06_18230"
     CDS             complement(<1..1822)
                     /locus_tag="PKO06_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV71648.1"
                     /translation="MATRAPAVTRILFIDVVGFSRKPMAIQKQVIQALTEMVQQTTAF
                     RRLDDSQRVSLPTGDGMAIAFFGAPEIPVACAIELQKAMRRHNAAHGPELELLVRMGI
                     NSGEVFTVLDINEHRNVVGDGINNAQRVMDFGDAWHILASEAVVHDLVADNPLYESCF
                     HDAGWFADKHGKRHHVYNLHAGDFGNPELPTRHRVETGPVAQASSPSPQPSVPAPPCA
                     PSEPPAPVPDNAVPVSAPTPSDPHAAGEDRAATIMLNGPAVNPQVQVTLVVEQGPEQG
                     RTFTFSEPEVFIVGRSKQAHFALSADDPAVSRKHFMLEIVPPRAYIQDFGSLNGTFVN
                     DRRVSQQELSDRDLIKVGRTSLRVSLQTQSSAPSCKCTKCGCAYLPSEAGKDGDNSVC
                     RACLRELELERAGRQPKTVTRVPATCCECGCDLSADANADGRAADLMPVASYLCDRCV
                     SASAATPELRSLDEYQLLRKLGEGGMGEVHLVRHSRTRRLGALKQVIQTGASEQVTLR
                     FKREMSIMEHLVHPRVVRLFAQDVLRHGNYFVSEFLDGGNASRLILQDYQGPVPAAIA
                     CRVLIDALSGLEFVHASGHVHRDLKPANILLAGKRSAEPGR"
BASE COUNT          415 a          602 c          572 g          373 t
ORIGIN      
        1 cccgaccggg ttccgccgag cgttttccgg caagcagaat gttcgccggc ttgaggtcgc
       61 gatggacatg cccggaagcg tggacaaact cgaggccgga cagtgcatcg atgaggaccc
      121 gacatgcaat cgccgccgga accggccctt gatagtcctg gagaatgagg cgtgaagcgt
      181 ttcctccatc caggaactcg gacacgaagt agttcccatg acgcagcaca tcctgcgcaa
      241 agagcctgac caccctcgga tgcacgagat gctccatgat cgacatctct cgtttgaaac
      301 gcagggtcac ctgttcgctc gcgccggtct gaatcacctg tttgagcgcc ccaagcctgc
      361 gggtgcgcga atggcgaacc agatgcacct ctcccatgcc accttccccg agcttgcgca
      421 acagctggta ttcatcgaga gagcgaagtt cgggggtcgc ggcagaagcg ctgacgcaac
      481 ggtcacagag gtagctggca accggcatca gatcagccgc gcgaccgtca gcattcgcgt
      541 cggcggacag atcgcaccca cactcgcaac aggtcgcggg aacccgggta accgtcttgg
      601 gttgtcgacc cgcgcgctcg agctcgagtt cgcgaagaca ggcccggcac acagaattat
      661 caccatcttt tccggcttcg gaaggcagat acgcacaccc acactttgta catttacaag
      721 acggagccga actctgcgtc tgcaggctca ctcgcagtga cgtgcgcccg accttgatca
      781 gatcacgatc gctgagttcc tgctgactca cgcgccggtc gttgacaaac gtcccattca
      841 gagatccgaa atcctgaata taggcgcgcg gcggcacgat ctcgagcatg aaatgcttgc
      901 gcgacaccgc cggatcatcg gcactgagag caaagtgcgc ctgtttggag cgaccgacga
      961 tgaacacctc cggttcggag aaggtgaagg tgcgcccctg ctcgggcccc tgttcgacca
     1021 ccagcgtcac ctgaacctgc gggttgaccg ccggcccatt cagcatgatc gtcgctgccc
     1081 gatcttcgcc ggccgcgtga gggtcgctcg gggtcggggc agaaacggga accgcattgt
     1141 ccggcacggg agcaggcggc tccgacggtg cgcacggggg ggcagggacg gaaggctgtg
     1201 gagatggcga cgatgcctgg gcaacagggc ccgtttcgac gcgatggcgc gtgggcaact
     1261 cggggtttcc gaagtcgccg gcgtgcagat tgtagacatg gtgacgtttt ccgtgtttat
     1321 cggcaaacca tccggcatca tgaaaacacg attcatacag ggggttatcg gctaccagat
     1381 catggaccac ggcttcagac gccagaatgt gccaggcatc gccaaaatcc atgacgcgct
     1441 gggcattgtt gataccgtct ccaaccacat tccgatgctc gttgatatcc agaaccgtaa
     1501 acacttcacc ggaattgatt cccatgcgca ccagcagttc gagttccggt ccatgagccg
     1561 cattgtgtcg acgcatggcc ttctgcaact cgatagcgca ggccaccgga atttcgggtg
     1621 cgccaaagaa ggcaatggcc ataccgtctc cggtcggaag tgagacccgc tgtgagtcat
     1681 cgaggcggcg aaacgccgtc gtctgctgaa ccatttcggt cagcgcctga atgacctgct
     1741 tctgaatggc catcggtttt cggctgaaac cgacaacatc gatgaagaga atcctcgtca
     1801 ccgccggtgc tctcgtcgcc acgcaacgcc tccatttcct gttttcggta ccctatgctt
     1861 ttcggcaggc acctgtcaaa ccacagaatc ccgcggactt cacagattca gatcgtgtgg
     1921 tgccgccggg tacggtatac tcactcaagt tcatcacatg gg
//
LOCUS       DAORHD010001025         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74312_length_1961_cov_2.689927, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001025 DAORHD010000000
VERSION     DAORHD010001025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74312_length_1961_cov_2.689927"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="PKO06_18235"
     CDS             <1..1111
                     /locus_tag="PKO06_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71649.1"
                     /translation="KTAARRSFLALEPRNTEQSSLIRCMGTAISPSPTLVFGKVYRSY
                     ILERGIRRKPPAPAGPEVPASAAALPFIPQDRFNDPQWPGTAEEAQQILKPACDNDWS
                     SYGQCMSTLIVAPCNEGLAYAFDTGGQNPRNRSLKPTFQTSELTKLGRSLKYSPQNPK
                     DLILDGPIELYRGSQQVYAGEVQQALANLGPLSLARCGREVNSTKDLLALFQELKRKG
                     ISTTGYYHVKGPFTWTADFNTLALGGVGIVADGDLRVSESIMPSTPATDPDLQVHPIV
                     LVSLKGNLIIGTNQPLHASLCAPLGELRLEGSRGLSLSGTLAVWKLNADVLNSAAEKT
                     IRYDPLYDWADQNAHVKTSRVMLEHQPLVFQAKLE"
     gene            1143..>1961
                     /locus_tag="PKO06_18240"
     CDS             1143..>1961
                     /locus_tag="PKO06_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71650.1"
                     /translation="MIKHLSLGLLLGLGMLIVPVASGQSNGLIVDLNLGKAGSASQLI
                     HAPLVGQPTRDSHWVYDRFPDGSKNPAFPPPAQAPQLPVQVGFPAVLVDVGQIFRPFP
                     DQVLVHPVGSDTDHTSQEVLLPGVTLQVDATLPWVKTGFVVKSGQKFYIQASGTWSNH
                     FDQAWCGPEGHLGMPTSDKSGHPPQPCSTANHGALVARIGTGGTGFAVGTSGEFTATR
                     DGEVQLMCNDTLEGPEGYTPGPTCILCENMGFSNNSHLGNNQGALTVTLSLFPFP"
BASE COUNT          415 a          585 c          541 g          420 t
ORIGIN      
        1 gaagaccgcc gcgcgtcgat cgtttctcgc tcttgagccg cgcaataccg agcagtccag
       61 cttgatccgc tgcatgggta cagcgatttc gccatcaccc accctggtgt tcggaaaggt
      121 ctatcgcagc tacattcttg aaagaggcat tcgtcgcaag ccgcctgccc ctgctgggcc
      181 cgaggtcccg gccagcgcag ccgccttgcc gttcatccct caggaccgtt tcaacgaccc
      241 tcagtggccg ggaaccgcgg aagaggcaca gcagatcttg aaacccgcct gcgacaatga
      301 ctggagttcg tatggacagt gcatgtccac attgatcgtg gctccctgca atgaagggct
      361 ggcctatgca tttgacacgg gtgggcagaa cccgcggaat cgctcgctaa aaccgacttt
      421 tcaaacatcg gagctgacaa agctgggtcg atcattgaaa tactcgccgc agaatcccaa
      481 agacctgatt ctggacggac caatcgagct gtatcgcggc tcacagcagg tctatgccgg
      541 tgaagttcaa caggcgttgg cgaatctggg gccactgtcg ctggcccgat gcggacggga
      601 agtgaattcc accaaagatc ttctggctct ctttcaagaa ctgaaaagga aagggatctc
      661 aacgaccggg tactatcatg tcaagggtcc gtttacctgg accgccgatt tcaacaccct
      721 ggcactcggt ggtgtcggaa tcgtcgccga tggggatctc cgagtttctg aaagcatcat
      781 gccatcgaca cccgcgactg accctgatct tcaggtgcac cccatcgttc tggtcagcct
      841 gaaggggaac ctgatcatcg gcaccaatca gcccctgcac gcgagtcttt gcgctcctct
      901 tggggaactc cgcctcgaag gctcgcgtgg cctcagtctg tctgggacgc tcgccgtctg
      961 gaaactgaac gccgacgtgc tgaattcggc tgcggaaaaa acgatccgtt atgatccact
     1021 gtatgactgg gctgatcaga acgctcatgt aaagacctct cgcgtgatgc ttgagcacca
     1081 acccctggtg tttcaggcaa aacttgaata acccgatcag aattcaggag ttgcccccta
     1141 ccatgatcaa gcatctctct ctcggtctgc tcctcgggct tggcatgctg attgtaccgg
     1201 tcgcctcagg ccagtcgaac ggtctgatcg tcgatctcaa cctcgggaag gccggaagcg
     1261 ccagtcaatt gatacacgcg cctctggtag ggcaacccac ccgcgactcc cattgggtct
     1321 atgatcgctt tcccgacggc tcgaagaacc cggcctttcc gccaccggcg caggctccac
     1381 aacttccggt tcaggtcggc tttccggctg tcctcgtgga tgtcggtcag atttttcgcc
     1441 cgtttcccga tcaggtgttg gtgcatcctg tcggcagcga taccgatcac acgtcccagg
     1501 aagtcctgct gccgggggtt acccttcagg ttgatgcgac tcttccatgg gtcaaaaccg
     1561 ggttcgtggt caagtcagga cagaaatttt acatacaagc cagcggaacc tggtcgaatc
     1621 attttgacca ggcctggtgc ggaccggaag gacacctggg catgccgacc agtgataaat
     1681 ccggtcatcc gccgcagccg tgttcgacgg caaatcacgg tgctctggtg gcgcggatcg
     1741 gaaccggggg aaccggattc gccgtgggta ccagcggcga gttcaccgca actcgcgatg
     1801 gagaggttca gctgatgtgc aacgacacat tagaaggacc ggaaggctat acccccgggc
     1861 ctacctgtat tctttgtgaa aacatggggt tcagcaataa cagtcacctg ggaaacaatc
     1921 agggagcgct gaccgtgacg ctctctctgt ttccttttcc a
//
LOCUS       DAORHD010001026         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74317_length_1961_cov_2.583945, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001026 DAORHD010000000
VERSION     DAORHD010001026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74317_length_1961_cov_2.583945"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PKO06_18245"
     CDS             complement(<1..416)
                     /locus_tag="PKO06_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HNV71651.1"
                     /translation="MHIPDGFLNIPTASVCGVLAVAGIGTALRRIDRDLPDRRVPLLG
                     LSAAFVFAAQMLNFPVAAGTSGHLIGATLCAILLGSEAAMISMSAVLILQCLIFGDGG
                     ITALGANLFNMALVAPLVGYSVWRFASRLFGSSTTA"
     gene            complement(457..921)
                     /gene="nikR"
                     /locus_tag="PKO06_18250"
     CDS             complement(457..921)
                     /gene="nikR"
                     /locus_tag="PKO06_18250"
                     /inference="COORDINATES: protein motif:HMM:NF002169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNV71652.1"
                     /translation="MSNTADTQLRRFSVCMPAELVTEVDNLVATHCFANRSHALSELV
                     RSHLTDRHGTEANSEVAATITLVYDHHKRDLQALLTDIQHDHQSLIIASMHVHLDHHR
                     CLEILTVRGNARLLRELSDRLLAVRGVSHGRMTVNALLSRKPPQQRTQRHRS"
     gene            1305..1868
                     /locus_tag="PKO06_18255"
     CDS             1305..1868
                     /locus_tag="PKO06_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71653.1"
                     /translation="MIKKYGAVPAPILHPPASIPPKPTLGTRLRTLLFSITGTRHSTS
                     GARFKACYANQRVILGAVEMYNLDHSATPKTRFSHADAATPAGDLVAGSYLKTPVTLV
                     DVACRYDVYGNPSDDGLVYCRHHGAFDDDQQQALRMAAGYVEGKTDGDQSLEVLVAVL
                     MLLTTLSCMGAWLYLKRQGAGTAEAAE"
BASE COUNT          441 a          502 c          612 g          406 t
ORIGIN      
        1 tgtgcggtgg tgctggatcc gaacagacgt gacgcgaacc gccagacact gtaacccacc
       61 agcggggcga ccagcgccat gttgaacaga ttggcgccca gcgcggtaat tccgccatca
      121 ccgaagatca ggcattgaag aatcaaaacc gcgctcatgg agatcatcgc ggcttcagat
      181 ccgagcagga tggcgcagag agtcgctccg atcagatgac cggacgttcc ggcggcgacc
      241 gggaagttga gcatctgggc cgcgaagaca aaagcggcgg agagccccag gagcggcaca
      301 cggcgatcgg gaagatcgcg atcgatgcgt cgaagcgccg tgccgatacc cgcgacagcg
      361 agaacgccgc agacggatgc ggtcggaatg ttcaggaaac catcgggaat atgcataata
      421 agatctttct ttggtgtaat gactcggacg ctgttgctat gaacgatgac gctgcgtgcg
      481 ttgctgcggc ggtttccggc tcagaagcgc attcacggtc atacggccgt gggagacgcc
      541 gcgcaccgcc agcagacgat cagacagctc tcgcagcagc ctggcgttcc cgcgcacggt
      601 gaggatttca agacaccgat ggtgatcgag atgaacatgc atcgaagcga tgatcagact
      661 ctgatgatcg tgttgaatgt cggtcaagag tgcctgcagg tcacgcttgt gatgatcata
      721 aaccagggtg atggtcgcgg cgacctcgct gtttgcctct gtgccgtgtc gatcggtcag
      781 gtgcgagcgt acgagctcag acagggcgtg cgaccggttg gcaaagcagt gtgtggcaac
      841 cagattatcg acttcggtga ccagttctgc gggcatgcac acgctgaagc gtcgcagctg
      901 ggtgtctgcg gtgttactca tgataacatt cgtaacacca acggcgagaa cagtcaagaa
      961 ttctgtgccg catcaggcag gattgaagat gtgtcacagt cattgccagg atttcggcct
     1021 gtgagaaatc gagagcccgg tggtcgcgtg attggtgata ccatggtggc gttcgggcag
     1081 ctgaaggttg gcagcagggc tgtcgttcac gtggcgatcg aggcgtgagt cagttcaaac
     1141 ggagaagaac gcgaatgttc gagaacaggc agagaacaag gatgagaaag gccggtgtca
     1201 ctctccgtgt agtctttttg gggtgtttgt ttctgttgtt gcctggattg gtcaggcagc
     1261 ctctgaatgc gcaagagaaa gcactgtcga aatgggcaga ggacatgatc aaaaagtacg
     1321 gggcagtgcc ggcaccgatt ctccatccgc cggccagcat tccgccaaaa ccgacgcttg
     1381 ggacgagact gcgaacactg ttgttctcga tcacgggaac caggcattcc acttccggag
     1441 cacgtttcaa agcctgttat gcgaatcaga gagtgatact cggcgcggtt gaaatgtaca
     1501 acctcgatca ttcggcgact ccgaagacac gcttttctca cgccgatgct gccacgccgg
     1561 ccggagatct cgtggcgggc tcttatctga aaaccccggt tactctggtc gatgttgcct
     1621 gtcggtatga tgtctatggc aacccttctg acgatggtct cgtctattgt cgccatcatg
     1681 gagccttcga cgatgatcaa caacaggcct tgagaatggc tgcaggatac gtcgaaggca
     1741 aaaccgatgg cgaccagtca ctcgaggttc tggttgctgt tctgatgctg ctgaccacgc
     1801 tctcgtgcat gggagcctgg ctgtatctga agcgacaagg cgcaggtaca gcagaggcgg
     1861 ccgaatgact acatctttcg agcggcttcg atgtagctct gtatcatctt gagcgtttcc
     1921 tgtgcggcct caacggggga ataggcagtg tcggaaatgc c
//
LOCUS       DAORHD010001027         1956 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74572_length_1956_cov_4.248816, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001027 DAORHD010000000
VERSION     DAORHD010001027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74572_length_1956_cov_4.248816"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1956
                     /locus_tag="PKO06_18260"
     CDS             <1..>1956
                     /locus_tag="PKO06_18260"
                     /inference="COORDINATES: protein motif:HMM:NF019311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HNV71654.1"
                     /translation="IERLIVGASDGTDHKAFVLDMGVQKLYKTIELASSPELIAADPS
                     GCWALAGPEGVAPSATSFHNFCLRVIDGVLATSPIGFTAGALTSISGGQQMIITSSYT
                     VQIRSPFSGGPILHPFIQSDLGPLNLAQGFFISAAMRPILTATPTAVAGLAGLYFGDD
                     VHSSATQARANDATVNFSWPGASPAGAAPYSVRWTGVLKPTVTTTYQLRVNSDDGARV
                     WLDGQLIIDDYYPWHAAQNADSAVISLTAGTVHPIAVEMYNPWSPGNVVLSWKVGAAA
                     FAVIPSANLGTTMPAQPAAFALTSPDGVKPYGISYFNGSDTHVFPYFPYAFDFPASPG
                     SQIAISPDDGVLAVMVPNVGATADGRRIDLYDINHDITPAKFGLRKFEFNHSAVVPSF
                     RRGAPTGTPTTIRTSPIKDGALLSVCGVCPDNSCTFEFWDDGEDHMSDAGFANKTWHF
                     FGKVFYKAPTIYSIEWRASADDGPRVDIDESNILTDWTTHFVRSEIGTETFSAPTADA
                     NSQREVRLRRFSMEAWNGGGGGNTIWADYMELKPGNHWGYKTAANVGNIYFPDVGDNV
                     QKFFHGYNPPRFIRSIQMKFGAAIPAGTGAPTFHASDTMIMRMNPNGESLVVIDRQFG
                     YLGEFNLYDNGSWSTHLPAGWTAESDVA"
BASE COUNT          409 a          574 c          545 g          428 t
ORIGIN      
        1 atcgaacgtt tgatcgtcgg agcgtcggac ggcacggacc acaaagcctt cgttctcgac
       61 atgggcgtac agaaactgta caagaccatc gaactggcca gttccccgga actgatcgct
      121 gccgacccga gcggctgctg ggcacttgcc ggcccggaag gggtggcacc aagtgcgacc
      181 tcatttcata acttttgtct gcgggtgatc gatggggtgc tggcaacaag cccgatcggg
      241 ttcactgcgg gggcgttgac ctcgatcagc ggtgggcagc agatgatcat aacgtcgtcc
      301 tacaccgttc agatccgatc ccccttttcc ggaggtccga ttctgcaccc gttcattcag
      361 tctgacctgg ggccactcaa tctggcacag ggcttcttca tcagtgcggc gatgaggccg
      421 attctcacgg cgaccccaac agcagttgcc gggcttgccg gcctttactt cggtgatgac
      481 gttcatagca gcgccactca ggcccgcgcg aacgatgcca ccgtgaactt ttcgtggccg
      541 ggtgcgtcac ctgcgggggc cgctccatac agcgttcgct ggaccggggt cctgaaaccc
      601 acggtgacga ccacgtatca gttgcgcgtg aactctgatg acggcgcgcg agtgtggctc
      661 gacggccaac tgattatcga cgactattac ccgtggcacg ccgctcagaa cgccgattcg
      721 gccgtgattt cactcaccgc cggcacggtt cacccgattg cggtcgagat gtacaatccg
      781 tggtctcccg gaaacgtggt tctgtcctgg aaagtcggcg cggccgcgtt cgccgtcatt
      841 ccatcagcca acctcggcac cactatgcct gcccagccgg ccgcgtttgc gcttacgtcg
      901 ccagacggtg tgaaaccgta tggaatctcg tatttcaatg gcagcgacac gcatgttttt
      961 ccctattttc cgtatgcgtt cgactttccc gcatcaccgg gctcgcaaat cgccatctcg
     1021 ccggacgacg gcgttctcgc cgtgatggtg ccaaacgtcg gcgctaccgc cgacgggcga
     1081 cgcatcgact tgtacgacat caaccacgac atcaccccgg ccaagtttgg attgcgcaag
     1141 ttcgaattca accactccgc cgtcgttccc agctttcggc gcggagcccc aaccggaaca
     1201 ccgacaacta tcagaacttc cccgataaag gatggcgcac tcctcagtgt ttgtggggtt
     1261 tgtccagaca acagctgtac atttgagttc tgggatgatg gcgaagacca catgtctgac
     1321 gctggtttcg ctaacaaaac ctggcacttt ttcgggaagg ttttttataa agcgcccacc
     1381 atttactcga tagagtggcg cgcttcagcg gacgacggac ctcgggtcga tatcgacgaa
     1441 tcgaatattt tgaccgactg gacaacacat tttgtgaggt cagagatcgg gactgagact
     1501 ttttctgcgc caacagccga cgcaaattct cagagagaag tccgtctccg aaggttttcg
     1561 atggaagctt ggaatggtgg cggtggtggc aacacgatct gggcagacta catggaactg
     1621 aagcctggaa accactgggg ttacaagact gctgcaaacg tcggcaacat ctactttcca
     1681 gacgtgggtg ataacgttca gaagttcttt cacgggtaca atccgccgag gttcattcgg
     1741 tcaattcaga tgaaattcgg cgcggccata cccgccggaa ctggggcacc tactttccat
     1801 gcaagtgata ccatgattat gcgaatgaac ccgaacggag aatcgctcgt ggttatcgac
     1861 cgacagttcg gctatctcgg cgagtttaac ctgtacgaca atggctcgtg gtcaacacac
     1921 ttgccggcag gttggaccgc cgagtcagac gtggct
//
LOCUS       DAORHD010001028         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74790_length_1953_cov_2.187039, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001028 DAORHD010000000
VERSION     DAORHD010001028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74790_length_1953_cov_2.187039"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKO06_18265"
     CDS             <1..771
                     /locus_tag="PKO06_18265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71655.1"
                     /translation="APATTPAGSLRGEIHARDLCFSYRGGPTVLQSVSFHFPAGVRVG
                     ICGPSGAGKTTLLCLLLRFYPPGSGTLLFDGRSINDYRAHDLRRRLGYVPQVPLLISG
                     TIAENVRFGNSQTTEAEIIALARELGVDDWVRILPQGYATPVSEAGANFSEGQKQRLG
                     LIRALVRDPDILILDEPGAMLDGIASQHILTYLSSAGRRRTTLIVTHDPLWLEHTDTI
                     LWFPEPGKVVQDTHQNLVATSSPYRNLLGITRAIESAG"
     gene            886..>1953
                     /locus_tag="PKO06_18270"
     CDS             886..>1953
                     /locus_tag="PKO06_18270"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV71656.1"
                     /translation="MTPEDRLLSLAALHNAASNHANELQDLIRGAINWDTIHQKAAKH
                     RIGCQVFQTLSIVAFDRVPELYRRHWQETAERSVRHNLTLVGELIRVIDLLDRHGILC
                     LSFKGPVLAALLHGTVALRPFADLDLIVATPEEARIAKELIVAEGYRVHESLTPRQEQ
                     LLLQTHCEYHLLHPEKQIYIDLHWNVFYDYFSYSFDLPRAFRESITVPVAHRALRSFR
                     PEDLLLLLSAHHARHAWETMLMIVDIADLLQTHPDLDWQYIENQAERVGGRRLLHLAL
                     FLAERVCTSHLPSYISSQARSDITVRRLAQLVQCRWFSSESGTAGMLDELLFHLRCRE
                     RLFHQLRFLLRGLFTPGPHDFA"
BASE COUNT          382 a          606 c          517 g          448 t
ORIGIN      
        1 gctcccgcaa ccactccggc cgggtcgctc aggggagaga ttcatgctcg cgatctgtgt
       61 ttctcgtatc gaggtggtcc gacggttctg cagtctgtct cttttcattt cccagccggg
      121 gttcgggtcg gcatctgcgg ccccagtggc gcaggcaaga ccaccctgct ctgcctgctg
      181 ctccgttttt atccgccagg ctccggcacc ctgctgtttg acggccgatc catcaacgac
      241 tatcgtgccc acgatctgcg gcgacggctg ggatatgtgc cgcaggttcc cctgctgatc
      301 agcgggacga ttgccgagaa tgtgcgattc gggaacagtc agacgaccga agctgagatc
      361 atcgctctgg cgcgagagtt aggagtcgac gactgggttc gcatcctccc ccagggatat
      421 gcaacccccg tcagcgaggc aggcgcaaac ttctcggaag ggcagaagca gcgcctcggt
      481 ctgattcgcg ccctggttcg cgaccccgac attctgattc tcgacgaacc gggtgccatg
      541 ctggatggaa tcgcatcaca gcacattctg acctatcttt cgtccgccgg acgtcgacgc
      601 accacactga tcgtcacgca tgatccgctc tggctcgaac acaccgacac gattctgtgg
      661 ttccctgaac ccggcaaggt ggtgcaggac acccaccaga accttgtggc cacaagttcc
      721 ccgtatagaa acctccttgg cattaccaga gccatcgaat cagcaggctg aaccgagtcc
      781 tatttcatga aactcttgca ccgcttctca tcacattttg ccaaccatcg atccccggag
      841 actcttcacg gcggagggac cctgagacgc atcacgaggg gtggcatgac cccggaagat
      901 cgcctgctgt cacttgccgc actccacaat gccgcttcga accacgcaaa cgagcttcaa
      961 gatcttatcc gcggggcaat caactgggac acgatacatc agaaggcggc taaacatcgt
     1021 atcggttgtc aggtgtttca gaccctctcg atagtagctt tcgaccgagt gccggagctc
     1081 taccgaaggc actggcagga gactgccgaa agaagcgttc gccacaatct caccctggtc
     1141 ggagaattga ttcgcgtcat cgatctgctt gaccgacacg ggatcctctg tctttcgttc
     1201 aagggcccgg tcctggccgc tctgttgcac ggcaccgtcg ccctgcgtcc gttcgctgat
     1261 cttgacttga ttgtcgccac accggaagag gcacgcatcg ccaaggagtt gatcgttgcg
     1321 gaaggatacc gggttcacga atcgctgacc ccgcgacagg aacaactgct tctgcagaca
     1381 cactgcgagt accatctgtt gcaccctgag aaacagatct atattgactt acactggaat
     1441 gtattctacg actatttttc gtattcattc gacctgccgc gtgcttttcg agagagcatc
     1501 acagttccgg tcgcccatcg cgcgcttcgg tcgtttcgcc ctgaagacct gctgctcctg
     1561 ttaagcgcgc atcatgcgcg acacgcttgg gaaacaatgt tgatgatcgt tgacatcgct
     1621 gatctgctgc agactcaccc cgacctcgac tggcagtata tcgagaatca ggccgaacgg
     1681 gtgggcggtc gtcgtttgct gcatctggcg ttgtttctcg ccgaacgcgt ttgtacgagt
     1741 cacctgccgt cttacatttc ttctcaggct cgttcagaca tcaccgtacg ccgtctggct
     1801 cagctggttc aatgccgctg gttttcctct gaatcaggaa cggctggaat gctcgatgaa
     1861 ctgctgtttc atctcagatg ccgggaacgc ctgttccatc agcttcgttt tctgctgcga
     1921 ggacttttca ccccgggccc gcacgacttc gcc
//
LOCUS       DAORHD010001029         1952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74846_length_1952_cov_3.124934, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001029 DAORHD010000000
VERSION     DAORHD010001029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74846_length_1952_cov_3.124934"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="grpE"
                     /locus_tag="PKO06_18275"
     CDS             <1..835
                     /gene="grpE"
                     /locus_tag="PKO06_18275"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV71657.1"
                     /translation="LKIDDKSPAAQKIVEDLKKQNREHEQTRAALPEQITQVLKDKSR
                     LEQQVIDLEAMIRRFTSEKLKRDSENLVNPPNPLAAEENIFFFELLTQIVLQVPDTVD
                     YRELRTKAEEGIAILERHRMIQLIPTVGHAFNDRQHKVVRSFQTGMLEDGTIIYEVNR
                     GYSFGDQIVQRALVWIAKSRFKCHSCGAQARGPDNFCPKCGLELCTPDGISKRKLTPL
                     PPTTEICLPLVDYYLEQRHFAKVEELLDYLPQEKQLLPATVKRRNRLVELKNTPVKEK
                     T"
     gene            832..1161
                     /locus_tag="PKO06_18280"
     CDS             832..1161
                     /locus_tag="PKO06_18280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HNV71658.1"
                     /translation="MSTQTNPGTCPQCSARLPEGVLFCTRCGKALETTPTADTEELLV
                     EETSEAPSGSTEPSTLHPTVQSAPESSPSPFSLLQKEIEFLDQQYPWNLISLACVWAF
                     LFLTYFV"
     gene            complement(1177..1791)
                     /locus_tag="PKO06_18285"
     CDS             complement(1177..1791)
                     /locus_tag="PKO06_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV71659.1"
                     /translation="MDRQTRDYYSRSAKKLAAEYRAASVEHLHQTLKELANTATTALD
                     LGCGSGREMAALATLGTRVWGADSSMEMLHEIQVSYPEFRDQLIQCCLPTRLPFRTAS
                     FELLLSVSVLMHLDIYDIPFVFSECRRVLKNQGKFLFSVPLTRTDIRNGRDPHGRQFT
                     MSASETWEKMVLDSGFSVEQRLREADGLGRSGTSWITFVCACRE"
BASE COUNT          464 a          535 c          534 g          419 t
ORIGIN      
        1 actgaagatc gacgacaaat ctcccgccgc tcaaaagatc gtcgaagatc tgaagaagca
       61 gaatcgtgaa catgagcaga cgcgtgccgc tttgccagag cagatcacgc aggttctaaa
      121 agacaagtcc agacttgagc agcaggtgat tgatctcgag gcgatgattc gccggttcac
      181 ttctgaaaag ctgaaacgcg actcagagaa tctggtgaat ccgcccaatc cgttggccgc
      241 cgaagaaaac atcttctttt tcgagttgct cacccagatc gtgctgcagg ttccggacac
      301 ggtcgattat cgcgaactgc gaaccaaggc cgaagaagga attgccatcc tcgagcggca
      361 ccgcatgatt caactcattc cgacggtcgg gcatgcattc aatgatcgac agcacaaagt
      421 ggtgcgttca tttcaaaccg ggatgctcga agatggaact atcatctacg aagtgaatcg
      481 cgggtattcg tttggggatc agatcgttca gcgcgccctc gtctggatcg cgaaatcacg
      541 cttcaagtgt cattcctgtg gagctcaggc gcgtgggccg gacaatttct gccccaagtg
      601 cggtctcgaa ctctgcaccc ctgatggcat cagcaaacgc aaactgactc cgttgccgcc
      661 aacgactgag atctgtctgc cattggttga ctactatctc gaacaacgcc attttgccaa
      721 ggtcgaagaa cttctcgact atctgccgca ggaaaagcag ttgctgccgg caacggtgaa
      781 gcgacgcaac cgcctggtgg aactcaagaa tactcccgtg aaggaaaaga catgagcact
      841 cagacaaacc ctggaacctg cccccaatgc tctgcccgat tacccgaggg cgttcttttc
      901 tgtacccgtt gcgggaaagc gctcgagaca acgccgactg cggatactga agagttgctt
      961 gtcgaagaga cttctgaggc tccatctggt tcaaccgaac ctagtacgct tcacccgacg
     1021 gttcagagcg caccagaatc ctccccttcc ccattttctc tcctgcagaa agagatcgag
     1081 tttctcgatc aacagtaccc ctggaatctg atctcgctgg cgtgtgtgtg ggcgtttctg
     1141 tttctgacgt atttcgtctg atacttcgtc agcagactac tctcggcaag cgcagacaaa
     1201 cgttatccaa cttgtaccgg aacgtccgag accatcggct tcacgaagtc gttgctcgac
     1261 gctgaagccg gaatcaagaa ccatcttttc ccaggtttcg ctcgcggaca tcgtgaactg
     1321 tcggccgtga gggtcgcggc cgttcctgat gtctgtccgc gtgaggggaa cggagaagag
     1381 gaacttcccc tggtttttca gcacgcgccg gcactcggaa aacacgaacg ggatgtcgta
     1441 aatgtccagg tgcatgagta cggaaacaga caaaagaagc tcgaaagaag cggtgcgaaa
     1501 cggaagtcgg gtgggcagac agcattggat cagttgatcg cggaattcgg gatatgaaac
     1561 ctgaatctcg tgcagcattt ccatcgagga atcagcgccc cagacgcgag tgcccagcgt
     1621 agcgagcgct gccatttcgc gacccgatcc acacccgaga tcgagagccg tggtcgcggt
     1681 gttggctagt tctttgagcg tctggtggag atgttcaacc gaggctgctc gatactctgc
     1741 agccagtttt ttcgcgcttc gagagtaata gtcgcgagtc tgccggtcca tgtctcacgg
     1801 acgttcatcg aggaaccacg cttgcgcggc tctcgacaga ctggaaaaac gaatcgtcag
     1861 attcggcgta ccgtctccgg gtttgcggta gtagagacaa ccgtgtttct gcacgatgat
     1921 cggcgcaggt gccgccggaa ctggggcgaa cg
//
LOCUS       DAORHD010001030         1951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_74907_length_1951_cov_2.975738, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001030 DAORHD010000000
VERSION     DAORHD010001030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74907_length_1951_cov_2.975738"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="PKO06_18290"
     CDS             <1..778
                     /locus_tag="PKO06_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNV71660.1"
                     /translation="LAKAEMLELMPESGFAVLPGDDPFLPTLKSRSRAQVVTFGFGTG
                     CDLRGVDVRMGPDGIDLVVEQRLRRFPLHLQLLGKHNALNALAALALFASCGHDLEEG
                     VAAMAEFRPVAARMESHEIEGMRIILDCYNANPSSMKLALEYLAICRGRRVAVLGDMR
                     ELGSVSEDLHREIGRHVCRQKLDVLVAVGDQAQFIAEAAIQEGMDAPCVHCCRDTDEA
                     AAALRLVLRQEDTVLLKASRGMYFEKIVKQLWPSLHIDLH"
     gene            789..1883
                     /gene="mraY"
                     /locus_tag="PKO06_18295"
     CDS             789..1883
                     /gene="mraY"
                     /locus_tag="PKO06_18295"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNV71661.1"
                     /translation="MFTGIVSALLIRILFALLTAFFMALIIGPRVIENLKARQIGQVI
                     REEGVQAHKKKAGTPTMGGIIILVPLIVTVLFWCRWNFSVGLVMAVTLFMGGIGLLDD
                     LTKVIKVRSLGLTPRQKLAGHISCGVVVALAMLYWPGLQALLQAPAGTPIAQPLVQAR
                     SVTMLPYWGLVDFGWLYLPLVVFVIVGATNAVNLTDGLDGLAAGTAAIALVPFLVVAY
                     LMGSVLISGALGTLHLPGVEELAVLAAALIGGCLGFLWFNGYPAEVFMGDTGSLALGG
                     ALASMAVVLRSEAFLAVIGGLFVVEALSVIIQVSYFKWSGGQRIFRMSPIHHHFELCG
                     WPETKVVLRFWMVGLILAVAGLALYLARVV"
BASE COUNT          338 a          487 c          634 g          492 t
ORIGIN      
        1 gctggccaaa gcggaaatgc ttgaactgat gccggaaagc ggtttcgcgg ttctgccggg
       61 agacgatccg tttctaccga ccctcaagag ccggtcgcgg gcgcaggttg tgacgttcgg
      121 cttcggtacc gggtgtgatt tgcgtggagt cgatgtgcgc atgggtccgg acggcatcga
      181 tctggtggtc gaacaacgct tgcgccgatt cccgcttcac ctgcagctgt tggggaagca
      241 caatgccctg aatgcccttg cggctctggc gttgtttgcg agttgcggac atgacctgga
      301 agagggtgtc gccgcgatgg cggagtttcg gccggtcgcc gcccgcatgg aatctcacga
      361 gatcgagggg atgcgcatca tccttgattg ttacaacgcg aacccttctt cgatgaagct
      421 ggcactggaa taccttgcga tctgtcgtgg ccgtcgtgtt gccgttcttg gggacatgcg
      481 cgaacttggg tctgtttcgg aagacctgca tcgtgagatt ggtcgccacg tgtgccgtca
      541 gaagctcgat gtcctggttg cagtcggtga tcaggcccag ttcatcgcag aagcagccat
      601 acaggaaggc atggatgctc catgcgtgca ttgctgcaga gatacggatg aggcagcggc
      661 cgcgctgcgg ctcgtcttga gacaggaaga tacggttttg ctcaaggcca gccgaggaat
      721 gtacttcgag aagatcgtga aacagctgtg gccaagcctg cacatcgatt tacactgagg
      781 atcctgacat gtttaccggt attgtgagtg cgctactgat tcgcatcctg tttgctctgt
      841 tgactgcgtt tttcatggca ctgatcattg gcccgcgggt tatcgagaat ctcaaggccc
      901 gtcagatcgg acaagtcatt cgtgaagaag gcgtgcaggc ccataagaag aaggcgggaa
      961 cgccgaccat ggggggcatc atcattctgg ttccattgat cgtgacggtg ttgttctggt
     1021 gtcgctggaa cttttccgtg ggtctggtga tggccgtgac gctcttcatg gggggaattg
     1081 gcttgctgga cgatctgacc aaggtcatca aagttcgatc tctcggattg accccacgac
     1141 agaaactggc tggccatatc agctgtggcg ttgtggtcgc tctggcaatg ctgtactggc
     1201 ctggcctgca agcgctgctg caggctccag ccggtacgcc catagcgcag ccgcttgtgc
     1261 aggcgcgatc ggtgacgatg ctgccatact ggggtctcgt agattttggc tggctgtatc
     1321 tgcctttggt cgtctttgtc atcgtcggag cgacaaatgc ggtgaatctg acggatggac
     1381 ttgatggact ggctgcagga accgccgcga ttgcgctggt cccctttctg gtggtcgcgt
     1441 atctgatggg aagcgtgttg atcagcggag cgctcgggac tcttcatctg ccgggcgtcg
     1501 aagagctggc ggtgctggca gccgctctga ttggcggttg tcttgggttt ctctggttca
     1561 atgggtatcc agccgaggtt ttcatgggcg acacggggtc gctggctctg ggaggcgcac
     1621 ttgcttccat ggcggtggtg ttgcgttctg aggcctttct ggctgtcatc ggaggcctct
     1681 ttgtcgtcga ggcgttgtcg gtcatcattc aggtcagtta tttcaaatgg tcaggtggcc
     1741 agcggatttt tcgaatgagc cccattcatc accatttcga actgtgcggg tggccggaaa
     1801 ccaaagtcgt gctgcgtttc tggatggtcg gattgattct ggcggttgct ggcctggccc
     1861 tttatctggc ccgcgttgtc tgagtgcccg ggcaaacggc cctaaaaaga aattccccag
     1921 cttgcgctgg ggaaaacagt ttggggtgtt a
//
LOCUS       DAORHD010001031         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75032_length_1949_cov_3.785639, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001031 DAORHD010000000
VERSION     DAORHD010001031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75032_length_1949_cov_3.785639"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PKO06_18300"
     CDS             <1..363
                     /locus_tag="PKO06_18300"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HNV71662.1"
                     /translation="RSAPGSVLSVHTSDGTYQLEVAVEDDPPFTLKGNQIETTINLNL
                     AQAMLGSKVKMRDPSGEEIILTIPAGSQPGDKLRLRGLGFSGADLIVKLDVQLPKNLP
                     EAQKKELADLFDRLGLRY"
     gene            513..>1949
                     /locus_tag="PKO06_18305"
     CDS             513..>1949
                     /locus_tag="PKO06_18305"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV71663.1"
                     /translation="MKAQKLSLVFLVLSLVLLAGAAAEARLSSHYQPLAGYVIMLDPG
                     HGGADPGAIGPTGLKESVTNLRVARYLRQLLQDDGAEVVMTREGDETVSLSDRVEKAR
                     QLKPDLFVSIHHNASLRPGAENKGEIYFNALDRGIPLQVADSMNEQLGQSNLASRTQL
                     IPGGFYVLRNNPAPAILTEAAYISVPESERVLRSGKGLTEEAQRFRLAIRQTFGTSGP
                     LRIKIISSMPAKTVSPFLNLLIESNKNIGKVEVRMTGPGNSSFGFERLPAIGNMYSLF
                     NLQPLPTGDYELTFICRGVDGSLSQKEILPVQVRLPVTEAVLLPVAPFIPEGLAGEFP
                     IRVLLKDANGKVNRQAIPFSVEYGSENLKAMTRPDGQGFFQITLQGTETDQVRLKATF
                     ETGQEQTLEIPVRKNEKTFVLGRVVSMPEKEGMEQVRISYGRDRVTTTAPGGYFFCEY
                     SKVFRNLKLHLQPPLGYASAAVMINSNGA"
BASE COUNT          399 a          573 c          577 g          400 t
ORIGIN      
        1 cgcagtgccc cgggcagcgt gttatcggtc cacacgtccg atggcacata ccagctcgaa
       61 gtggcggtcg aagatgaccc gcccttcact ctgaaaggca accagatcga aacgacgatc
      121 aatctcaatc tggcccaggc catgctcggc agcaaggtga agatgcgaga tccgtcgggc
      181 gaagagatca ttctgaccat tcccgcgggt agtcagcccg gcgacaaact gcgcctgcgc
      241 ggcctgggtt tctccggggc cgatctgatc gtgaaactcg atgtgcagct gccgaaaaac
      301 ctgccagagg cacagaaaaa agagctggct gacctgttcg atcgtctggg tctgcgctac
      361 tgaacttttc ggctcgccat ccgctttccc gccaacgagt cgaggccgcg ttccggccgt
      421 tgccactcgg tctgttcgca gatcgaaaac gcctgatttc ctgaatgacc tgagggtacg
      481 tgccgtcgcg gttttgtgtt accattccgg gtatgaaggc acaaaaactt tctttggtgt
      541 ttctggtgtt gtcgttggtt ctgttggcgg gggctgccgc cgaggcacgc ctctcttctc
      601 actaccagcc attggccggg tatgtgatca tgctcgatcc cgggcatggc ggggctgacc
      661 cgggggcgat cggcccaacc gggctcaagg agtcggtcac caatctgcgg gtggcgcggt
      721 atctgcgtca gttgctgcag gatgacggag ctgaggtcgt catgacccgc gaaggcgacg
      781 aaaccgtgtc attgagtgac cgggtggaaa aagctcgcca actcaagccc gatctattcg
      841 tttccattca ccacaatgcc tcgctgcggc ccggcgccga aaacaagggc gagatctatt
      901 tcaatgcgct cgatcgcggc attccccttc aggtcgctga ttccatgaat gaacaactcg
      961 gacagagcaa cctggcgtca cgcacgcagc tgattccggg cgggttctac gtgctgcgca
     1021 acaatcccgc gcccgcgatt ctcaccgaag ccgcgtatat ctccgttccc gagagcgaac
     1081 gcgtgctgcg gtcaggcaaa ggtctcaccg aagaggcaca gcgttttcgt ctggccattc
     1141 gccagacctt tggtaccagc ggcccgctga gaatcaagat catctcttcg atgccggcca
     1201 agaccgtcag cccgtttctg aacctgttga tcgagtcgaa caagaacatc ggcaaggtcg
     1261 aagtgcgcat gaccggaccg gggaactctt cctttggatt cgagcggttg ccggccatcg
     1321 ggaacatgta ctctctgttc aatctgcagc cgcttccaac cggagactat gaactgacct
     1381 tcatctgccg cggtgtcgac gggtcattgt cgcagaagga gattctgccg gtgcaggtgc
     1441 gcctgcccgt gaccgaagcc gtactgctgc ccgtggctcc cttcattccc gaaggattgg
     1501 ccggtgagtt cccgattcgc gtgctgttga aagatgcgaa cggcaaagtc aatcgccagg
     1561 ccattccgtt ttcggtcgag tatggttcgg aaaatctgaa ggccatgacg cgaccggacg
     1621 gacaagggtt ctttcagatc acgctgcagg gcacagagac agatcaggtc aggctgaagg
     1681 cgaccttcga aacgggccag gaacaaaccc tcgagattcc cgtgcggaag aacgagaaga
     1741 cgttcgtgct cgggcgcgtc gtatcgatgc cggaaaagga aggcatggag caggtacgca
     1801 tctcctacgg ccgagaccgc gttaccacga cggccccggg gggatacttc ttttgtgagt
     1861 attcgaaggt gttccgcaat ctgaagctgc atctgcagcc ccccctggga tacgcttccg
     1921 ccgccgtcat gatcaactcg aacggggcg
//
LOCUS       DAORHD010001032         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75035_length_1949_cov_3.586061, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001032 DAORHD010000000
VERSION     DAORHD010001032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75035_length_1949_cov_3.586061"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PKO06_18310"
     CDS             complement(<1..513)
                     /locus_tag="PKO06_18310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV71664.1"
                     /translation="MTLIELLVVSTILVLIASLTLPVSQIIRQRDKESRLREILRQVR
                     DQGIGLGTQSGGYANYVNYYLNTAIDPNLPNDASRSHAIASGLQLGLSYPRTPTLLEN
                     PIGEVLHFPTDNSGGSVTVTIPRRFIRKIPPHPFADWYPGVRWEFRIISSDTGAGLTS
                     EWYASETGDPW"
     gene            complement(589..>1949)
                     /locus_tag="PKO06_18315"
     CDS             complement(589..>1949)
                     /locus_tag="PKO06_18315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV71665.1"
                     /translation="FVATLAVALLVLLSIPGILPATSTNITYFKSLDLIRKVIEIIKS
                     DYVEENIEEQKLIYGAIEGMLKTLEDPYTRFMEPKSFKEMQTETQGEFGGLGIVISIK
                     NRLLTVVSPIEDTPAWRAGIRSGDMILRIDNKDVTDIPLHDAVRLLRGPEGSKVTISI
                     VRENEKEPRDVTLVREIIKIPSIKYWIIKPNIGYIRLTQFIQTSSEDLEKAMLALEKG
                     GAKALILDVRNNPGGLLTAAVEVCRKFIPKGDIVSIKGRDGEKNTYGSFFQSHPLIPM
                     VLLINEGSASASEIVAGALKDNRRAVIIGKKSFGKGSVQTVISLTDGSAIALTTALYY
                     SPAGVNIHKKGIEPDIEVELPRLNEKEREDAAKSMKAEEDILRQRDADRRDGKVSSET
                     VELSGWAEPPDSLSRGSLQPYVVNKYDTQLTAAVKVLNFEDAFGQMLKRLENPDSSGS
                     AK"
BASE COUNT          399 a          574 c          527 g          449 t
ORIGIN      
        1 ccaggggtct ccggtttcag aagcgtacca ctctgaagtc aggcctgccc cggtgtccga
       61 cgagatgatt cgaaactccc acctcactcc cgggtaccag tcagcaaacg ggtgaggcgg
      121 aatcttccgg ataaaccggc gcggaatggt cacggtgaca ctgcccccgc tgttgtccgt
      181 cggaaagtgc agcacctccc cgatcgggtt ctcgagcaac gtgggggttc ttggatacga
      241 gagtccgagt tgcaaacctg aagcaatcgc atggcttctg gaagcatcgt tcgggagatt
      301 gggatcaatt gcggtgttga gataatagtt cacatagttc gcgtagccgc cgctctgcgt
      361 cccaagaccg attccctgat cgcgcacctg acgcagaatc tcgcgcaggc ggctttcctt
      421 gtcacgctgg cgaatgatct gcgatacggg cagggtcagc gaggcgatca acaccagaat
      481 cgtcgaaacc acgagcagct cgatcagcgt cacccctgat cgagttgatt cgcggcttcg
      541 attacagcct gccctcacct ctcccccctc aatcgcttgt gccggcactc acttcgccga
      601 acccgaactg tccgggttct caagacgttt cagcatctgg ccgaaagcgt cttcgaagtt
      661 cagaaccttc accgcggctg tcagctgcgt gtcgtactta ttgaccacat acggctgcag
      721 actgccgcgt gacaggctgt ccggcggctc agcccaccct gacaactcga ccgtctcgct
      781 ggaaaccttg ccatcgcgac gatccgcatc tcgctgacgc agaatgtctt cttccgcttt
      841 catgctcttg gcggcatctt cgcgctcttt ctcgttgagt ctcggcagtt cgacctcgat
      901 atcaggctcg atgcccttct tgtgaatgtt gacgccagcc gggctgtaat acagcgcggt
      961 ggtcagtgcg atggccgaac cgtcggtcag cgaaatgacg gtctgtaccg aacccttgcc
     1021 gaacgacttc ttaccgatga tgacagcacg ccgattgtct ttcagagcgc cggcgacgat
     1081 ttcagacgcg gaagcggaac cttcgttgat cagcagcacc atcggaatca gtggatgcga
     1141 ctggaagaac gagccatacg tgtttttctc gccatctcga cccttgatcg agacgatgtc
     1201 gcccttggga atgaacttgc gacacacttc gaccgcggcc gtcagcagac caccgggatt
     1261 gttgcgcacg tcgagaatca gcgctttggc cccgcctttt tcgagcgcca gcatggcctt
     1321 ttcgaggtct tcagacgagg tctggatgaa ctgggtcaac cgaatgtagc caatgttcgg
     1381 tttgatgatc cagtatttga tgctcggaat cttgatgatc tcgcgaacca gcgtcacatc
     1441 acgaggttct ttctcattct ctcggacaat cgaaatcgtc accttgctgc cttcgggccc
     1501 gcgcagcagc cgtactgcat catgcagcgg aatatccgtg acatctttat tgtcgatgcg
     1561 cagaatcatg tcgccggacc gaatgcccgc acgccaggca ggcgtgtctt cgataggaga
     1621 aacgaccgtc agcagccggt tcttgatcga aatgacgata cccaggccgc cgaactcccc
     1681 ttgagtttcg gtctgcatct ctttgaacga cttcggctcc atgaagcgtg tatacgggtc
     1741 ttcgagtgtt ttgagcatgc cctcgatggc accgtagatc agcttctgtt cttcaatgtt
     1801 ttcttcgacg taatcactct tgatgatctc gatgaccttg cgaatcagat cgagtgactt
     1861 gaagtacgta atgttcgtcg acgtcgccgg gagaatgccg ggaatcgaca gcaggaccag
     1921 cagcgcgacg gcaagtgttg cgacgaaag
//
LOCUS       DAORHD010001033         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75042_length_1949_cov_3.324710, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001033 DAORHD010000000
VERSION     DAORHD010001033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75042_length_1949_cov_3.324710"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKO06_18320"
     CDS             complement(<1..660)
                     /locus_tag="PKO06_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71666.1"
                     /translation="MKRSVGILLAVLALTVSPVWSASQEELKYLGYSDEITNFSEEQL
                     RSTHKGETEAPPAVAQPLPQVRGDRAYEEATAVQEQLTELGKARSLTAETPDRSQEVK
                     DRIGTYWQGLNNDETAKVQVLSEEFKNELKVKLESALQEAGYAIRRLDLVDLPPNLGT
                     PQVRAVIRVVKPLKTNNGYQEIQKNLGQIKDICNVVGNVNGINYLGELSTFVAENPRN
                     QY"
     gene            complement(732..1262)
                     /locus_tag="PKO06_18325"
     CDS             complement(732..1262)
                     /locus_tag="PKO06_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNV71667.1"
                     /translation="MAFRRHIFVPNKRDYIRGRRRPTVECILCSILKGDPQVESLLVW
                     HDENFAVSVNLYPYNAGHLLVFPARHVFDLRELHPHEVLGMFELTRHFLKLLDHVYEP
                     AGYNIGCNMGDASGASIEHFHQHIVPRYPKELGFVDIVAGAKILIEDPQVTMEKLRHA
                     VREDPVRLPPIPENQP"
     gene            complement(1329..>1949)
                     /locus_tag="PKO06_18330"
     CDS             complement(1329..>1949)
                     /locus_tag="PKO06_18330"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNV71668.1"
                     /translation="IYVVTRGKDSEDLSRFNYPDIANTSVHEAYPGHHLQQTGQNLHP
                     SRIRCLVSGNETVEGWAHYCEEEMKRLGFENGDENVFIQTVDESWRAARVLIDVNLHF
                     KSWNAEQAAQFLRQNTTMDQESAESEIKWYVQSPGYPLSYLAGKHLIYRLKETLRRQF
                     GSDFRDRAFHDAVIYEGGIPLSLAGEFYPAFLKEELRQSPSAIVLR"
BASE COUNT          400 a          543 c          559 g          447 t
ORIGIN      
        1 gtactggttg cgcggattct cggcgacaaa tgtggacagt tcaccgagat agttgatgcc
       61 gttcacgttt ccgacgacat tgcagatatc cttgatctga cccaggttct tctgaatctc
      121 ctgataaccg ttgttcgtct tgaggggttt cacgacgcgg ataaccgcgc gcacctgcgg
      181 agttcccagg ttgggaggca gatcaacgag atccaggcgg cgaatggcgt agccggcttc
      241 ctgcagagcg ctttccagtt ttaccttcag ttcattcttg aattcttctg aaagcacctg
      301 aacctttgcg gtttcgtcgt tgttcaagcc ctgccagtag gttccgatgc gatctttgac
      361 ttcctgcgat cgatcaggag tttcggcggt cagtgaacgg gctttcccca gttcggtcaa
      421 ctgttcctgc acggccgtcg cttcttcata cgcgcggtcg ccacgcacct gcggcaaagg
      481 ctgagccacg gctggtggag cttcggtctc gcctttatgg gtcgagcgca gctgttcctc
      541 cgagaagttg gtgatttcgt cagagtaccc gagatatttc aactcttcct ggctggcgga
      601 ccagacgggt gacacggtca aagcgagtac ggcgagcaga atccctacag atctcttcat
      661 gacatattcc tccggggaat ttttgtttcg gcgggaagta taccacaaac cccggagaat
      721 gccagagatg atcatggctg gttttccggg atggggggca gccgcaccgg gtcctcgcga
      781 acagcgtggc gcagcttttc catggtgact tgcgggtcct cgatgagaat cttggccccg
      841 gcgacgatat cgacgaatcc cagttctttc gggtatctgg gtacgatgtg ctgatggaaa
      901 tgttcgatac tggcccccga agcatcgccc atgttgcagc cgatgttgta accggccggc
      961 tcgtagacgt gatcgagcag cttcagaaag tgccgcgtca gctcgaacat gcccagcact
     1021 tcgtgtggat gcagctcacg caggtcgaag acatgccgcg ccgggaacac cagcagatgc
     1081 cccgcattgt aagggtacaa gttcactgac acggcaaagt tctcatcatg ccagacaaga
     1141 agactctcaa cctgagggtc gcccttcaga atcgagcaga gaatgcactc gaccgtcggg
     1201 cgacgacgcc cgcggatgta atcccgcttg ttgggaacga agatatgccg tcgaaatgcc
     1261 atgcgtgcag aatattcagt ccgtgcggcc ctgtcaagaa gcgaaagccg cgccgttctg
     1321 cgagaatctc agcgcagcac gatcgccgat ggtgactgac gcagttcttc cttcaggaat
     1381 gcggggtaga attcccctgc cagcgagaga gggataccac cctcgtagat gaccgcatca
     1441 tggaaggcac gatcgcggaa atcactgcca aactgacgac gcagggtttc tttcaggcgg
     1501 tagatcagat gtttccccgc cagataactg agaggatatc cgggcgattg cacataccac
     1561 ttgatctcgc tctcggccga ctcctggtcc atcgtggtgt tctggcgcag aaactgggcg
     1621 gcctgctccg cgttccagga tttgaagtgc aggttgacat cgatgagcac acgcgccgcg
     1681 cgccaggatt catcaacggt ctgaatgaac acattctcgt ccccgttttc gaagccgagg
     1741 cgtttcattt cctcttcgca gtagtgcgcc catccttcca cggtctcgtt tcccgacacc
     1801 aggcagcgaa tgcggctcgg atgcagattc tggccggtct gctgcagatg atgtccggga
     1861 taggcttcgt gtacgctggt attcgcaatg tccgggtagt tgaagcgcga gaggtcctcg
     1921 gaatccttgc cacgggtcac gacatagat
//
LOCUS       DAORHD010001034         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75064_length_1949_cov_2.462513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001034 DAORHD010000000
VERSION     DAORHD010001034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75064_length_1949_cov_2.462513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..550)
                     /locus_tag="PKO06_18335"
     CDS             complement(11..550)
                     /locus_tag="PKO06_18335"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNV71669.1"
                     /translation="MRNFLLLLVLTVLTVPVFAQVGPRIMLDDSFKGEKIELRQVSIE
                     TRLAGHVAETRMTMAFHNPGSRNLAGDLVFPLPEGAAVSGYALDVNGVLVDGVAVEKE
                     KARVVFEKEMRKGVDPGLVEQVGGNTFKTRVFPIPPRGTRTIRVSYVSEPSNSGTKSQ
                     FLIPVRFGKIDGPRNDPVA"
     gene            complement(634..1236)
                     /locus_tag="PKO06_18340"
     CDS             complement(634..1236)
                     /locus_tag="PKO06_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71670.1"
                     /translation="MMSGWYNTGASYQVLAWVAIGLALLFTVMVGKLVVWPVTRRWLR
                     KDESTAQEAKGVILALCLLLVGPWWIGVLWFWSGIVWLQVQPDGGWNLRNAYGWPIHV
                     VMPSTERRLSLDERVRSYTTYTTEQVRQDSRGIGVDARLEMRTGQKIALDIEEYLDPR
                     LATGSAFFLRLGYTPEHVSVSSGTMIFDWHTYTASGPVFP"
     gene            1733..>1949
                     /locus_tag="PKO06_18345"
     CDS             1733..>1949
                     /locus_tag="PKO06_18345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71671.1"
                     /translation="MNLSFRAKIIGLFLIIGLLPICLISFLSYQSARQIIFDMVAGEV
                     LARAHAQRSDLDAQISERVSDSILWANL"
BASE COUNT          418 a          635 c          493 g          403 t
ORIGIN      
        1 gcgtggaccg ctacgcaacg gggtcgttcc gggggccgtc gatcttcccg aaacgcaccg
       61 gaatcagaaa ctgactcttc gtcccgctgt tcgagggctc gctcacatag ctgacacgaa
      121 tggtgcgggt gccacgcggt ggaatcggaa acacccgggt cttgaacgta ttgccgccga
      181 cctgttcgac caaccccggg tcgactcctt tgcgcatctc cttctcgaac acgacgcgag
      241 ctttctcttt ttcgaccgca acaccatcga ccagaacgcc gttcacatcg agggcatacc
      301 cgctgaccgc cgccccttcc ggcaacggaa acaccagatc acccgcgagg ttacgagagc
      361 cgggattgtg aaaagccatg gtcatgcgcg tttcggccac atgaccggcc aggcgcgtct
      421 cgatcgagac ctggcgcaac tcgatcttct cgcccttgaa cgagtcgtcg agcatgatcc
      481 ggggcccgac ctgggcaaac accggcaccg tcagcacggt caacaccagc aacaagagaa
      541 agtttcgcat cctgcaaacc ctccttactc gggtcttatc tcatcagaat ctatcacagc
      601 cggcgaaaga aacgcagcat caggaatgcg gattcacgga aaaaccggtc cgctggccgt
      661 gtaggtatgc caatcgaaaa tcatcgttcc cgagctcaca gacacgtgtt ccggcgtgta
      721 ccccagacgc aaaaagaacg ccgaacccgt ggccagacga gggtcgagat actcttcgat
      781 gtcgagtgcg atcttctgac cggtacgcat ctcgagccgc gcatccacac cgatccctcg
      841 tgagtcctgc cgcacctgtt ccgtcgtgta cgtcgtgtag gatctcacgc gttcatccag
      901 gctgagtcga cgttccgtcg acggcatgac cacatgaatc ggccagccat acgcattgcg
      961 caggttccag ccaccatcgg gctgcacctg cagccagacg attccgctcc agaaccagag
     1021 aacgccgatc caccaggggc ccaccagcag cagacacaga gcgagaatca ctcccttcgc
     1081 ctcctgagcg gtcgactcgt ctttccgcag ccaccgtcgc gtcaccggcc acacgaccag
     1141 cttcccgacc atcaccgtaa aaagaagggc caggccaatc gccacccagg ccagtacctg
     1201 atacgatgct ccggtattgt accatccact catcatcttg tttcccttct ggatttcatt
     1261 ccgaggatat ccttttcttc gcgccccgtc gagagttttg tccagtctcg gacccacgcc
     1321 tcacctggca agccccgtcc tctgagcaaa atgctgtcgc tgtcactgac gatttcccgc
     1381 gcattctttc cgcccgtata gctggaggca tcagatagtg ttcgccagac gtattctgag
     1441 atcctggcct aaagtcatcc tgtacaactc cgatcgggac aaaatcccct tccttatcct
     1501 ctttgacgac acgcaggggt tctgttaatc tggcagagca gcgggcccat gtcagcccct
     1561 tcatgagatc tgtaccacta cggatgtaag gaatgagtca gcagttcttc tgggccagtg
     1621 agaaggatcg agacgcggtc agtccggtgt tatcggtgat acactgtcag catacgaacg
     1681 gcgcgttcat gcacctcagc gagtcacgcc gctcagggag cgcccagctc gtatgaatct
     1741 gagttttcgc gcgaaaatca tcggactgtt cctgatcatc gggctgctcc ccatttgtct
     1801 gatcagcttc ttgtcgtatc agtccgcccg tcagatcatt ttcgacatgg tggcaggcga
     1861 agttcttgcc cgtgcccacg cacaacgttc cgatctcgac gcgcagatca gcgaacgagt
     1921 gtctgacagc attctctggg caaacctcg
//
LOCUS       DAORHD010001035         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75155_length_1947_cov_3.448732, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001035 DAORHD010000000
VERSION     DAORHD010001035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75155_length_1947_cov_3.448732"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="PKO06_18350"
     CDS             <1..1543
                     /locus_tag="PKO06_18350"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HNV71672.1"
                     /translation="FVILVIGGFIGLAYPSHSTPAMIAAVVGMLVAIAAMAVTPGMMR
                     DFFTSRSTWLWVNDVILVLSIIGIAVLLSYIGFRRHIRYDFTRDRLFSISDQTIAVLR
                     NLKQEVHITAFFPTGQMEGKMIEDLVKECQRWNEKLSFKMVDPYRDPLTVKNMAVKTD
                     RSVIVACENNRKDIFYDEMFVVPNPMMRGREPPKFQGEQAIMSAILNVTQGKKRRIVF
                     SNTNGEAQIRGFTAEGLAGIAQLLDKENFEVGEVDLAAGVPTDTTILAIVGPTKPFHP
                     QALQNVRDFVQKHKGMLLLALNYDFRDESLERMLSEIFGTRANPELLINPQKLFNDLT
                     IVAPNYEEHRIVRDLKAANIAVLMQMCRGLSVDPKPEWDTKQFLRTRSDQIYAKRALD
                     KVLAGNVDFDPSSDVRGPFTLGIAYEGREAASGTRAIVLGNASFFTNQFLRFQGNGDL
                     FLNTVNWLVGQEELISIRPKTVEFGTINLTAADINTVKYLSIFAGPLLIMLIGGTVWW
                     TRRRV"
     gene            1547..>1947
                     /locus_tag="PKO06_18355"
     CDS             1547..>1947
                     /locus_tag="PKO06_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71673.1"
                     /translation="MKKRFIPTVVALVVFLLLFLYANLYEVHEIAEPGKDLPVSLAKI
                     AATDVKKLTWKTAGAPDVVVECASGATMPKYRLVQPLALRAETPEVEGVMRHFEDLKS
                     ERTIMAAATDTAAFGISSQSPAIALETGSGTT"
BASE COUNT          397 a          554 c          593 g          403 t
ORIGIN      
        1 gttcgtcatt ctggtcattg gcggattcat cgggctcgcg tatccgagtc actcgactcc
       61 ggcaatgatt gctgcggtcg tcggcatgct cgtcgcgatc gcggcaatgg ccgtgacccc
      121 aggcatgatg cgcgacttct ttacctcacg cagcacctgg ctctgggtca acgatgtcat
      181 cctggtgctg tcgatcatcg gtatcgccgt gctgctctcg tacatcggct tccgtcgcca
      241 cattcgctac gatttcactc gagaccgcct gttctcgatt tctgatcaga ccatcgccgt
      301 gctgcgcaat ctgaagcagg aagttcacat taccgccttc ttcccgaccg gacagatgga
      361 aggcaagatg atcgaggacc tggtgaagga gtgccagcgc tggaacgaaa agctctcgtt
      421 caagatggtc gacccgtatc gcgacccgct gaccgtgaag aacatggcgg tcaaaacgga
      481 tcgttccgtg atcgtggcct gcgaaaacaa tcgcaaagac atcttctacg acgagatgtt
      541 cgtggtgccg aacccgatga tgcgtggccg tgagccgccg aagtttcagg gggaacaggc
      601 catcatgtcg gcgattctga acgtgacgca gggcaagaag cgccgcatcg tgttcagcaa
      661 caccaacgga gaagcccaga ttcgcgggtt caccgccgag gggttggccg gcattgcgca
      721 actgctcgac aaggagaact ttgaggtcgg cgaggttgat ctggcagccg gcgtgcccac
      781 cgatacgacg attctcgcga tcgtggggcc gaccaaaccg ttccacccgc aggcgctgca
      841 gaacgtgcgt gacttcgtcc agaagcacaa gggcatgctg ctgctggctc tgaactacga
      901 cttccgcgac gagtcgctcg agagaatgct gtctgagatc tttggcacgc gggcgaaccc
      961 cgaactgctg attaacccgc agaagctgtt caacgatctg accattgtcg cgccgaacta
     1021 tgaggagcat cgcatcgtgc gcgatctgaa ggcggcgaac atcgccgtgc tgatgcagat
     1081 gtgccggggt ctctcggtcg atcccaagcc ggaatgggac accaaacagt ttttgcgcac
     1141 ccgcagtgac cagatctatg ccaaacgcgc gctggacaag gttctggcgg ggaacgtcga
     1201 tttcgatccg tcgagcgacg tgcgcggccc gttcaccctg ggtatcgcgt atgagggccg
     1261 tgaagcggct tccggaacgc gtgcgatcgt gctgggcaat gcctcgttct tcaccaacca
     1321 gtttctgcgg tttcagggga acggggacct gttcctgaat acggtcaact ggctggttgg
     1381 gcaggaggag ctgatctcga tccgtccgaa gacggtcgag ttcggcacga tcaacctgac
     1441 cgcggccgac atcaatacgg tcaaatactt gtcgatcttc gccggcccgc tgctgatcat
     1501 gcttatcggc ggaacggtct ggtggacgcg caggagggtg taagccatga agaaacgatt
     1561 cattccaacc gttgttgcgc tcgtcgtctt cttgctgctg tttctctacg cgaatctgta
     1621 cgaggtgcat gagattgccg aaccagggaa agatctgccg gtgagcctgg ccaagattgc
     1681 ggcgaccgat gtcaagaaac tgacctggaa aaccgccggc gctcctgatg tcgtcgtcga
     1741 gtgcgcttcg ggtgcgacta tgccgaagta tcgactggta caaccgctgg cgttacgtgc
     1801 cgagactcct gaggttgagg gagtgatgcg ccactttgaa gacctcaagt cggaacggac
     1861 gatcatggct gccgcgacgg atacggccgc cttcggtatc agcagtcagt ctccggcaat
     1921 cgctctcgag acggggtcgg gaacgac
//
LOCUS       DAORHD010001036         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75163_length_1947_cov_3.018499, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001036 DAORHD010000000
VERSION     DAORHD010001036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75163_length_1947_cov_3.018499"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..755)
                     /locus_tag="PKO06_18360"
     CDS             complement(129..755)
                     /locus_tag="PKO06_18360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71674.1"
                     /translation="MKMKWLIATVVVVFLGATLEAGPTKEQLKKLYLEYKAAKEKNQQ
                     NENPADGSSGTANIASPTPPTSPAQTTIHRTGVHISPQVLSPDQQQRLAQLLETADHP
                     STKNQPAKKRGDDCMVHQLIELGKTAAREQQFDYAVAYLKKAVELDPDNIEARYCLSI
                     AYMHTERKNLAVQEYEEIKRIVAAHEALAERLLKYIQPVKEKPQNMFK"
     gene            complement(778..1848)
                     /locus_tag="PKO06_18365"
     CDS             complement(778..1848)
                     /locus_tag="PKO06_18365"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71675.1"
                     /translation="MTTKNPNNSDNGRQDISELLSAAEQGDTDAQVELAFCFAKGIGC
                     QPDEKQAFRWYQEAARRDNLQAQFELANCHEFGRGTECNSTKAARWYRKAADQGHAEA
                     QCMLGNLTMGGYGVQKDLEEAARLFRLAIANGSEEARKRLVICEYKIRIEAEFRARRT
                     FLPCPRDPGAKGLEDGFGYYSPRDYGKPRSPEWVENHPTPWTGQKPDNEQLGPLEDGG
                     AAKMTIDGDLWTRVPIEVTWSLAESDANAYVTCYKSTPQPSPRCIHTEFTFQGHTIHI
                     DYQRRVSFGPLSGFEEFKFFLDGKFYSRGGGFVCYGVWPHAWPAALRTPEFAIICNRF
                     FGSGILPEFKHLTAWLITRKKP"
BASE COUNT          379 a          567 c          490 g          511 t
ORIGIN      
        1 tgttcttctg cagtgcaaag accgcattat tacgttgaat ctccttttcg ctgatcgttt
       61 tccgacacat tccggaaaac cagcgacacc gaaccggcac gagaaagctt tcaggacgcg
      121 cgaaggtttc acttgaacat gttctgtggc ttttccttca cgggctgaat gtatttcaac
      181 agtcgttcgg ccagcgcttc gtgagccgcc acgattcttt tgatttcctc atattcctgc
      241 acggccaggt tcttcctttc cgtgtgcatg tacgcaatac tcaggcaata acgcgcttcg
      301 atattgtccg gatcaagttc gaccgccttc ttcagatacg ccaccgcata gtcgaactgc
      361 tgttctcttg ccgcggtttt gcccagctct atcagctggt gaaccatgca gtcatctccg
      421 cgcttttttg cgggttggtt tttggtcgag ggatggtcgg cagtctcgag cagttgtgcc
      481 agacgctgtt gctgatccgg actgagcacc tgaggggaaa tgtgcactcc agtcctgtga
      541 attgtcgtct gagccggtga ggtcggcggt gtcggcgatg caatattcgc cgttccggaa
      601 gatccgtcgg cggggttctc gttctgctga ttcttttctt tggccgcctt gtattccaaa
      661 tacagctttt ttaattgttc tttggtcgga cccgcttcca gggtcgcccc cagaaacacc
      721 accacaacag tagcaatcaa ccacttcatc ttcacgtttt tccctccttc agcaggttca
      781 aggctttttt cgagtaatga gccacgccgt cagatgcttg aactctggca aaatgccaga
      841 cccgaaaaat cgattgcaga tgatcgcgaa ctcgggagtt ctcagagcgg ctggccaggc
      901 gtgcggccaa acaccatagc agacaaaccc cccacccctc gaatagaact tcccgtcgag
      961 gaagaacttg aactcttcga aaccactcag gggaccaaat gatacacgac gttgataatc
     1021 gatatgaatg gtatggcctt gaaatgtgaa ttctgtgtga atgcacctgg gactcggctg
     1081 tggtgtcgac ttgtagcaag tgacataagc gttggcatcg ctttctgcca gtgaccaggt
     1141 cacctcgatc ggcacgcggg tccacaaatc gccgtcaatg gtcatcttcg ccgctccacc
     1201 gtcttcgagc ggaccaagtt gttcattgtc aggtttctga cccgtccacg gcgtcgggtg
     1261 gttttcgacc cattctggcg atcggggctt tccataatcc cgaggggagt agtatccaaa
     1321 gccgtcctcc aaccctttcg ctccggggtc tctcggacac ggcagaaagg tgcgccgcgc
     1381 gcgaaactct gcttcgatgc gaatcttgta ctcgcagatc accaggcgtt ttcgtgcttc
     1441 ctcagagccg ttcgcgatcg ccaaccgaaa caatcgtgcg gcttcttcaa ggtctttctg
     1501 gacgccatac ccgcccatgg tcagatttcc gagcatgcac tgggcttcgg catgcccctg
     1561 atcggcggcc ttgcgatacc agcgcgccgc ctttgtgctg ttacattccg tgcctctgcc
     1621 gaattcgtga cagttggcaa gctcgaactg tgcctgcagg ttgtcccgtc tggccgcctc
     1681 ctgataccag cgaaaagctt gcttctcatc cggttgacag ccgattccct tcgcaaagca
     1741 gaaggccagc tccacctggg catcggtgtc accctgttcc gccgccgaga gcagttccga
     1801 gatatcttgt cggccgttgt ccgagttgtt cgggtttttg gtcgtcatcc tctgctcctt
     1861 tccgagtcgt tcgattcgaa atcctttgca tttcgccagg aacctacata caacacattc
     1921 cggaaatttt cctcgatgcg taggccg
//
LOCUS       DAORHD010001037         1943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75438_length_1943_cov_3.635593, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001037 DAORHD010000000
VERSION     DAORHD010001037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75438_length_1943_cov_3.635593"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="PKO06_18370"
     CDS             complement(<1..748)
                     /locus_tag="PKO06_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNV71676.1"
                     /translation="MTPEQEARQRIDRQFDEAGWKVQNRDEANLAAGRGVAIREFKLK
                     SGHGFADYLLFVDGHAVGVLEAKPTDFTLTGVEVQAAKYAQGLPDELDAPIRPLPFCY
                     LSTGAVTRFSNRLDPLPRSRTIFQVHRPETLAEWLSATPLADWLGSAATSASPSTATP
                     TDTSTASATPATANRPSTLRGRLRALPPLVPGSLYANQIRAVTGLEKSLYEDRPRALI
                     QMATGSGKTIAAITAVYRLIKFGGARRVLFL"
     gene            complement(922..1191)
                     /locus_tag="PKO06_18375"
     CDS             complement(922..1191)
                     /locus_tag="PKO06_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV71677.1"
                     /translation="MSKYKPYINLGPGDHLKAEMEEKGWNLKDLADVLNVSEDQANRL
                     LMSDAQLTINVAKSLSQAFGQSPEFWINLATISQDREQEALKKAT"
     gene            complement(1263..>1943)
                     /locus_tag="PKO06_18380"
     CDS             complement(1263..>1943)
                     /locus_tag="PKO06_18380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV71678.1"
                     /translation="ACDRLYSNSDPRDFRTMILFTDGRDETPVTRQQMSIRSLDEALN
                     FARKKQVRIIAVGMGDQIDQNILKKLSSETGGWYMYAPKAQALFAVYERISTLMKAEK
                     HFQLAYQSPNQDRNGEKREIRVTVQTPSGPHERQLDYTAPLPLKTKPVSTIGIAMPGD
                     PFGTTTPPDASAGAQVSQPAQPVTPVEPPKPAFEIKIREGQQTTGLDLPPFPDAPPAS
                     DTPTGQFR"
BASE COUNT          328 a          559 c          631 g          425 t
ORIGIN      
        1 cgagaaacag cacgcgccgt gcgccgccga acttgatcag ccggtagacg gcggtaatgg
       61 ccgcgatcgt cttgcccgag ccggtcgcca tctgaatcag cgcgcggggc cggtcttcat
      121 acagagactt ttcgagaccg gtcacggccc ggatctgatt ggcatacaaa ctacccggca
      181 cgagcggcgg cagggcgcgt aatcgccctc gcaacgtgga cggccggttt gcggttgctg
      241 gtgtcgcgga agcggtcgaa gtatcggttg gggtggcggt cgaaggcgaa gccgaagtcg
      301 cggcactgcc gagccagtcg gcaagcggcg tagcgctcag ccattcggcg agcgtttcgg
      361 ggcgatgcac ctgaaagatc gtgcgactgc gcggcagagg gtcgagacga ttggagaacc
      421 gcgtgacagc gccggtcgag agatagcaga acggcagggg ccgaatcggg gcgtcgagct
      481 cgtcgggcag accctgcgca tacttcgcgg cctgcacttc gacgccggtc agcgtgaagt
      541 cggtcggctt ggcttcgagc acgccgaccg cgtgcccgtc gacgaacagc aggtagtcgg
      601 cgaacccgtg gccgctcttc agcttgaact cgcgaatcgc gaccccgcgg cccgccgcca
      661 gattcgcctc gtcgcggttc tgcaccttcc acccggcctc atcaaactgc cggtcgatgc
      721 gttgccgtgc ttcctgttcc ggtgtcatgc cattccttcg cgctctggtt ctctctcggt
      781 gccgtcgaaa aacgacaccc ccgattctac tcttcagcgc cttctgacac aacgcccagg
      841 atcaccccgt ttcgctcttt ccagcgccgg ttgtcagccc tacccaccgg aggatatctg
      901 cccctcgtgc gagagcgcgc ttcaggtcgc ctttttcaac gcttcctgct ctcggtcttg
      961 gctgattgtc gccaggttga tccagaactc tgggctttgg ccgaaagcct gactcaggct
     1021 tttcgcgaca ttgatggtca gctgagcatc gctcatcaga agccggttgg cctgatcttc
     1081 gctcacgttg agaacatcag cgagatcttt gaggttccag cctttttctt ccatctcggc
     1141 tttcagatga tcaccggggc cgagattgat gtacggtttg tatttcgaca tcgtaaatga
     1201 tcttctcttc gaattggttc gttgcattca gttcttccgt tagccgcacg cagaatgcgt
     1261 cgtcagcgga attggccggt gggggtgtcg gaggcggggg gcgcgtcggg aaaggggggc
     1321 aggtcgagac cggtggtttg ctgaccttcg cggatcttga tttcgaaggc cggctttggt
     1381 ggttcgacgg gtgttacggg ctgagccggc tgcgacactt gagcgccggc tgaagcgtca
     1441 ggcggggtgg tggttccgaa gggatcgccg ggcatggcga tgccgatcgt ggaaaccggt
     1501 ttggttttca gaggcaaggg ggcggtgtaa tcgagctggc gctcgtgcgg gcctgaaggg
     1561 gtctgcaccg tgacgcggat ctctcgcttc tcgccgttgc ggtcctggtt tggagactga
     1621 taggcgagct gaaaatgctt ttcggccttc atcagcgtgg agatgcgctc atacacggcg
     1681 aacagcgcct gtgctttagg tgcgtacatg taccagccgc ctgtttctga agagagcttc
     1741 ttgagaatat tttgatcgat ctgatctccc atgccgacgg cgatgatccg cacctgcttc
     1801 ttgcgcgcga agttcagagc ttcgtcgagg ctgcgaatcg acatctgctg gcgggtgacc
     1861 ggcgtctcgt cgcggccgtc ggtaaacagg atcatggtgc gaaagtcacg cgggtctgag
     1921 ttggaataca accgatcgca ggc
//
LOCUS       DAORHD010001038         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75585_length_1941_cov_2.943796, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001038 DAORHD010000000
VERSION     DAORHD010001038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75585_length_1941_cov_2.943796"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKO06_18385"
     CDS             complement(<1..664)
                     /locus_tag="PKO06_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71679.1"
                     /translation="MKPRHLRRLATAWVLLVVFSTQIVFAEVVRSDGQPKATSIPISK
                     EVVRTDGQPRATVPAVTKEVVRTDGQPRITVPGISKEVVRTDGQPRVTTPAVTKEVVR
                     TDGQPRSTTPAVTKEVVRTDGQPRTTGTPAINPFVGEIAVGVSLLVTYGPQIIALAAT
                     VATLAAAVHTIGAAGKSSLDVVKSIGSKLKSLLQLLLGPLGISTGMTKTGVEDVSAHL
                     LDV"
     gene            complement(844..1380)
                     /locus_tag="PKO06_18390"
     CDS             complement(844..1380)
                     /locus_tag="PKO06_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71680.1"
                     /translation="MNIRVFLIISLFFIGMAVHGESTAPEVAPATTDTAIVRESMDAD
                     TSFGLAGFNTTSGKFDRNADGKIDLIVVDRNADGRGDYWATDRNFDGLIDDYQYDRNF
                     DGRIDQWEYDTFHNGVPEKIYVDSNGSGRPDMYAVLNPYTRTYTWYGNLKALSGSGFS
                     SGIRPRRGLNRGRGGDSE"
     gene            complement(1463..>1941)
                     /locus_tag="PKO06_18395"
     CDS             complement(1463..>1941)
                     /locus_tag="PKO06_18395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV71681.1"
                     /translation="FTDFGLRPGTGTAAPEALIVVVGVTLWLFLSVNDSTDLLTSITN
                     PGISQYLLLEQKWWVLVLCLTAGGFFLPLVEETFYRGMCLAALESAGWNRFFSLCLSA
                     GIMALVQPASYMTAAFFQSLLCAWWYQRSGSVIPGLLLRGGLTVILMMHYCLFSLK"
BASE COUNT          442 a          516 c          574 g          409 t
ORIGIN      
        1 tgacatcgag caggtgagcc gaaacgtctt caacgcctgt cttcgtcatg ccggtgctga
       61 tgccaagagg cccgagcagc agttgcagca ggctcttaag cttgctgccg atgcttttca
      121 caacgtcgag tgagctcttt ccggcagcgc caatagtatg taccgccgcg gccagagtgg
      181 cgacggtggc tgccaatgcg atgatctgag gtccgtaggt gaccagcagc gaaacgccga
      241 cggcaatctc accgacaaac gggttgatgg cgggggttcc agtggtacgc ggttgaccgt
      301 cggtgcgtac aacttccttg gtgacggccg gtgtggttga acgcggttgc ccgtcggttc
      361 gaaccacttc ttttgttacg gcaggcgtgg tcacacgtgg ctgtccatcc gtgcgaacca
      421 cttctttgct gatgccggga acggttatac ggggctgtcc gtcagtgcgc acgacttcct
      481 tggtgacggc gggtacggtg gcacgtggct gaccatcggt tcgtaccact tctttggaga
      541 tcggaatgct ggtcgctttc ggttgaccgt cagaacgtac cacttcggcg aagacgatct
      601 gggtcgagaa gacgacgagc agaacccatg cggtggcgag acgacggaga tgtcggggtt
      661 tcatggcggc ctccttagca gacagaaagg taacggtatc ttttcattat acggaaatac
      721 cgggttttcg tgtcaaatga atcggcgaaa aaagaaaagc cccccggctg caaatgaatg
      781 agcccgcggg agctgaattt ccccggttgt ccgggggagt atctttcagg ggtattctga
      841 acgctactct gagtcaccgc cacggccgcg gttcaagccg cgccggggtc gaatcccgga
      901 tgagaatcct gacccagaga gagccttcag gtttccatac caggtatacg tacgagtgta
      961 cgggttcaac acggcataca tgtcgggtcg tcccgaacca ttggaatcaa catagatctt
     1021 ttcgggaact ccgttatgga aggtgtcgta ttcccattga tcgatgcggc catcgaagtt
     1081 tcggtcatac tggtagtcat cgatcagacc gtcgaagttg cggtcggttg cccagtagtc
     1141 gcctcgcccg tccgcattgc gatcgaccac aatcaggtcg attttgccgt cggcattgcg
     1201 atcgaacttt ccggaagtcg tgttgaagcc ggcgaggccg aatgatgtgt cagcatccat
     1261 ggactcacgc acaatcgcgg tatcggtcgt tgcaggtgcg acctcgggag cggtcgattc
     1321 accatgaacg gccattccga tgaaaaagag tgaaatgatg aggaagaccc gaatattcat
     1381 gcgttgcacc tccgttgtgt cgacaagtcg aagaaagtat tcgggtttcg acgttatgca
     1441 aggaaaattt ttcgaaacca cttcacttca atgaaaacag gcagtagtgc atcatgagaa
     1501 tgaccgtcag accgccacgc agcaacaggc ccgggatcac cgaccctgag cgctgatacc
     1561 accaggcaca cagcaggctt tggaaaaagg ccgcggtcat atacgacgct ggttgtacca
     1621 gggccatgat tccggcgctg agacaaaggc tgaaaaatcg gttccagccc gctgactcga
     1681 gtgccgccag acacatgcct cgatagaacg tctcttcgac cagcgggagg aaaaagccgc
     1741 ccgcggtcag gcaaagaacc agaacccacc atttctgctc aagcaggaga tactgtgaaa
     1801 ttcctggatt cgtgatggag gtgagcagat cggtcgaatc attgaccgac aggaacagcc
     1861 acagtgtcac tccgaccacg acaatgaggg cttccggggc cgcggttccg gttcctggcc
     1921 gcaggccgaa atcggtgaaa c
//
LOCUS       DAORHD010001039         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75902_length_1936_cov_3.583200, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001039 DAORHD010000000
VERSION     DAORHD010001039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75902_length_1936_cov_3.583200"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1936)
                     /locus_tag="PKO06_18400"
     CDS             complement(<1..>1936)
                     /locus_tag="PKO06_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71682.1"
                     /translation="GQKAQAAGKIEEAVEAFEDAYEAFPGNVQALLLWGDMLCEIGMY
                     GRAFEVLNKIPMASLLPNGQAQVNLLKGKVALASGSLPIAAAFLNDSLKSSPGFGVAR
                     VRLALLQLCLGNSRQARQVADDLNSLEGLNYRERAIALLLDLALDNVARALDNAREMS
                     GSMAKIGGEDGEILLLSLWQIPVVLVLSWFPLAGSHALFGLYFLILLCGLAFLASRLC
                     GSSPLWHAGVFVGLAIVHMLVSRVLLQSELYVSILREGCDFSDPFWVMPRVVHAMHGL
                     TLALFVVFPAFLLLPPSARPRQHELYSIWFFSFWFLVTVAVFQARISLASQLLLMAGC
                     ALPAIGAAFLMPLGKFLLFVVSSKIGMGEKFEEVATSTSGGGVGFTECKILETKSAPL
                     LEKEQFEEVVSMARKAFAAQPRRSFVALWVAQIKALIELEEMYDAEKSLAEFQRAFPP
                     QPSGEVGQLLNAYLKMNAADHAGALNLLNSVSEQQAKRFTPEQAAWSLLVLGRCYMHL
                     EDPVQAHIELSKALEVGRSPLSKVYALYDLMELELRMDRLEWAQKWAIQAASLKGGPK
                     TTSLSKVIQSMLASAQGNLPEALQLATAACTALGRHGRAWRWLAHLHLLNGAPRDAES
                     ILENKLTAGTADALFLMQEIT"
BASE COUNT          485 a          590 c          505 g          356 t
ORIGIN      
        1 gtgatttcct gcatcagaaa aagagcatcg gctgtgccgg cagtcagttt gttttcgaga
       61 atactttccg cgtctcgcgg agcaccgttc aacaggtgca ggtgtgccag ccatctccag
      121 gcacgcccgt ggcgtcccag tgcggtacat gccgcagtgg ccagttgcag tgcctcgggc
      181 aggttccctt gagccgaggc aagcatcgac tgaatcacct tgctgaggct ggtggttttc
      241 ggcccaccct tcagagaggc ggcttgaatc gcccacttct gcgcccactc caggcgatcc
      301 atgcgcagtt ccagttccat caaatcgtac aacgcataca ctttggacaa gggagatcgg
      361 cccacttcaa gcgctttcga caattcgatg tgcgcctgca ccggatcttc aagatgcata
      421 tagcagcgcc cgagaaccag cagcgaccag gccgcctgtt ccggcgtaaa acgctttgcc
      481 tgctgttcgg aaacgctgtt caacagattc agcgctccgg catgatcggc cgcgttcatc
      541 ttcagatagg cattgagaag ctgtcccact tcacccgaag gctggggcgg aaatgcgcgt
      601 tggaactccg ccagactctt ttcggcatcg tacatttctt caagctcgat cagcgccttg
      661 atctgagcca cccataaagc gacgaagctg cgtcgcggtt gagcggcaaa ggcttttcgc
      721 gccatgctga ccacttcctc gaactgttcc ttttcgagaa ggggcgcgga cttggtctca
      781 agaattttgc attcggtgaa accgactccc ccaccagaag tactcgtggc aacttcctca
      841 aacttttctc ccatgccgat cttcgaactc acaacaaaga gcagaaactt tccgagtggc
      901 atcagaaagg ccgctccgat cgccggcaga gcgcatcccg ccatgagcaa gagctgcgac
      961 gcgagcgaaa tccgggcctg aaacacggca actgtcacga gaaaccagaa actgaaaaac
     1021 cagatgctgt acaactcatg ctggcgtggt ctggcagagg gaggaagcag gagaaatgcg
     1081 ggaaaaacaa cgaagagagc cagagtcagc ccatgcatgg catgcacgac acgcggcatg
     1141 acccaaaacg gatcagaaaa atcgcaccct tcacgtaaaa tggaaacgta caactcgctc
     1201 tgaagtaaaa cccggctcac cagcatatga acgatcgcca atccgacaaa cacccccgcg
     1261 tgccagagtg gtgacgaacc acacagacga gacgccagga acgcgagccc acacagcagg
     1321 atcagaaagt acaggccgaa cagggcgtga ctacccgcca acgggaacca ggaaaggacc
     1381 aagacgacgg gaatctgcca cagagacagg agaagaatct cgccatcttc tccgccgatt
     1441 ttggccatcg agccagacat ctcacgcgcg ttatcgagag ctcgcgccac gttgtcgaga
     1501 gcaagatcga gcagcaaagc gatcgctcgt tctcgatagt tcaatccctc cagactgttc
     1561 agatcatcag cgacctggcg ggcctggcga ctgttcccca ggcagagctg caacagagca
     1621 agtcgcacac gcgccactcc aaagccgggg ctggacttga gcgagtcatt cagaaacgca
     1681 gccgcaatgg gcaacgagcc tgatgccaga gccacctttc ccttcagcag attcacctgg
     1741 gcttgcccgt tgggcagcaa cgaggccatg ggaatcttgt tgaggacctc aaacgcacga
     1801 ccgtacatgc cgatttcgca caacatgtcc ccccacagca gaagcgcttg aacattcccc
     1861 ggaaaagctt cataggcatc ttcaaaggcc tcgacggcct cttcaatttt tccagcggcc
     1921 tgagccttct gaccct
//
LOCUS       DAORHD010001040         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_75934_length_1936_cov_2.395003, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001040 DAORHD010000000
VERSION     DAORHD010001040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75934_length_1936_cov_2.395003"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PKO06_18405"
     CDS             complement(<1..718)
                     /locus_tag="PKO06_18405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71683.1"
                     /translation="MKLLHEFEPVFNPTASGADAEFPASNAIETEYDPGVPWIADSYI
                     ADAWLMVETTNIIDSVFLNRCNFPQARIQWNATDEWSAPTVDLEVALVRDDAKNRKGW
                     FPITGVTGPGYLKVFIPAGQALDDSGTVPQIGNLICGQAVTIPIVAEFDPRLVQDINS
                     FKTFYGGYHEEPIGRAQHLISMKIGDDLDPVRNMPKTWALGVLFADLGNVGEAWLVYP
                     ARDWSRPIRSLEDAGLSYAFR"
     gene            complement(720..1736)
                     /locus_tag="PKO06_18410"
     CDS             complement(720..1736)
                     /locus_tag="PKO06_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71684.1"
                     /translation="MQALKDMQFSYTSVVGHKSGNGIWSKKTTTYEAIGVAEAQASLA
                     KFTELSEKATTEAAARQFEIALKQITSPLEALNMTISDLRDAVSRATDEQKQQLDYEL
                     KQALAQKEQMLGQYQANWFATRLSTAAASAGTISAPPMESLAGISVPNGIRANLIAGQ
                     PNHLVQLGLLEKQAKLDYSLAKKGLTANDSEAALEAYLKERLEMNSRALKTYSALMEK
                     ARTDAFAAKENTAAAEQALQEFVQAQNAYYAADLEGLQIKQQLQAEIKAKQEAQKNDQ
                     ILDALTWIGEQTKIGGVEVLVLAPGQASTEDRLVKIRNNADPAGAKVINEIIEANRTV
                     TRRR"
BASE COUNT          343 a          562 c          566 g          465 t
ORIGIN      
        1 cccggaaagc gtagctcaac ccggcgtctt caagcgatcg aatgggccgc gaccagtctc
       61 tggccgggta aacgagccag gcttctccga cgttcccgag gtcagcaaac agcacgccca
      121 gagcccacgt tttcggcatg ttccgtaccg ggtccaggtc gtcgccgatc ttcatcgaga
      181 tcagatgctg tgcacgcccg atcggttctt cgtgataccc gccgtagaac gtcttgaagc
      241 tgttgatgtc ctgcaccaga cgcgggtcga actcggcaac gatcggaatt gtcactgcct
      301 ggccgcagat caggttgccg atttggggaa cggtcccgga atcgtccagg gcttgaccgg
      361 cgggaatgaa caccttgaga tagccagggc cggtcacacc cgtgatcggg aaccagcctt
      421 tgcggttttt ggcgtcgtcg cgcaccaggg caacttcgag atcgacggtg ggcgcgctcc
      481 actcatcggt tgcgttccac tgaatacgag cctgcggaaa gttgcatcga ttcaggaaaa
      541 cagagtcgat gatgttcgtg gtttcgacca tcagccacgc atcagcgata taactgtcag
      601 cgatccaggg tacaccgggg tcgtattcgg tctcaatggc gttcgatgcg gggaactccg
      661 cgtcagcgcc ggatgccgtc gggttgaaca ccggttcgaa ttcgtgcagc agtttcattt
      721 catcgtctcc gggtcacggt gcggtttgct tcgatgattt cgttgatcac cttcgcgccc
      781 gccgggtcgg cattgttgcg aatcttcacc aggcggtcct cggtcgaagc ctggccgggt
      841 gccagaacca gcacttcgac accgccgatc ttcgtctgtt cgccgatcca ggtcagggca
      901 tcgagaatct ggtcgttctt ctgggcttcc tgcttggctt tgatctcggc ctgcagctgc
      961 tgtttgatct gcaacccttc gagatcggcg gcatagtagg cgttctgagc ctgcacgaac
     1021 tcctgcagag cctgttcggc ggctgcggtg ttttctttgg ccgcgaacgc gtcggtgcga
     1081 gctttttcca tcagcgccga atacgtcttg agggcgcgag aattcatctc cagccgttct
     1141 ttcagatacg cttcgagagc cgcttcgctg tcgtttgcgg tcaggccctt cttcgccagg
     1201 ctgtaatcga gcttggcttg tttttccaag agtccgagtt gcacaaggtg attcggctga
     1261 ccggcaatga ggtttgccct gatgccattc ggaaccgaga taccggccag ggattccatg
     1321 ggcggggcgc tgatcgtgcc tgccgaggct gctgctgtgc tgagtcgcgt cgcgaaccag
     1381 tttgcctggt attggccgag catctgttct ttctgtgcca gcgcctgttt cagctcgtaa
     1441 tcgagctgct gcttctgctc gtcggtcgcc cgcgacaccg cgtctcgaag gtctgagatc
     1501 gtcatgttca gcgcttcgag cgggctggtg atctgcttca acgcaatctc aaactgtctg
     1561 gcagctgctt cagtcgtcgc tttttcggaa agctcggtga acttcgccag gctggcctgg
     1621 gcttcggcga cgccgatcgc ttcatacgtg gtcgttttct ttgaccagat gccgtttccg
     1681 ctcttgtggc cgaccaccga ggtatacgaa aactgcatgt ccttgagggc ctgcatcgtc
     1741 gatgccgaga cgccgaccag cgacgagatc tacacaagtc ctcacatcat ccgttccccc
     1801 gtagaaggac tgaaccttgc tgatcaagac tttagccagc aaggttcagt ccttctacgg
     1861 gggaacggat gatgtgtaca aggtggcagg cttcagagcc ttactcaaca agggttattc
     1921 tctagaggaa gccacc
//
LOCUS       DAORHD010001041         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76097_length_1933_cov_3.209265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001041 DAORHD010000000
VERSION     DAORHD010001041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76097_length_1933_cov_3.209265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1933)
                     /locus_tag="PKO06_18415"
     CDS             complement(<1..>1933)
                     /locus_tag="PKO06_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNV71685.1"
                     /translation="WQRIRSLALAHSGRWGEANPSREPFTSRESQGFWGSPAEDFGVT
                     SAFPLESTTALAFQLTPTFQELLRIWQLLECGQADTAEAAWQSACQNQDWIPLESIRV
                     EAALLCCRGDYLGAYELISGTTGFRPLYPLCRLAIWLGREPAIAEYVEKRFADLTNPS
                     DRFWATELLLGAGRKEPVRTWLNSTTASTRTWRSDDWWQVWALETNPEESESRLSPTS
                     RHTIAACLRERQDMKRRARLQRWCRNTWLHSRASSWSRQVASAIADLFQFPREERLAA
                     GSPPDPVKQQHLEKGFPQELLSKDLRAAITASRSADTALEAFDQLVHDAGLPVLLAVM
                     GEFNAGKSTLINALLGTNALPRGNLPTTPLPCTLRYGDYPLAVLIANDGQRQYLPIEQ
                     LPGLFGHSSVPVAGSESPLVDPRGQLPFALRQVREIEIVLPIELLKHFWIIDTPGLNS
                     GNTSHQRATEALVRRADAVLWVFDANQAGRASELNALHRLVPPHALRLIAVNRLDEID
                     PDETEDVLADVRRALGDERLPLFAVSAKQALLPHSEANRSGIDELRRHLLERIGPDER
                     SFKERSVQARIRLLLEQVREKEAQEREAVQDYPNRLKHELETLCTRSQRIVSDWFARH
                     SDEIRRELEPFCRAWSTVA"
BASE COUNT          362 a          569 c          609 g          393 t
ORIGIN      
        1 ttggccaccg tggaccaggc gcgacagaac ggttcgagtt cgcggcggat ttcatcggaa
       61 tgacgtgcga accaatcgga aacgattctc tgtgagcggg tacacagagt ttccaactcg
      121 tgtttcaggc gattcggata gtcctggacc gcttctcgtt cctgtgcttc tttttcgcgc
      181 acctgctcca gaagcaggcg tattctggct tgtacgctcc gttccttgaa agatcgctcg
      241 tctggtccga tgcgttcgag cagatgccgg cgcagctcgt ctatgcccga tctgtttgcc
      301 tcactgtgtg gcagcagtgc ctgttttgcc gaaaccgcaa acagtggcag ccgctcgtcg
      361 ccgagtgcgc ggcgcacgtc tgccagcacg tcttccgttt cgtcagggtc gatctcgtcg
      421 aggcgattga ccgcaatcag acgcaaggca tgagggggga cgagtcgatg caacgcgttc
      481 aactctgacg ctcttcccgc ctgattggcg tcgaacaccc agagaacggc gtccgcacga
      541 cgcacgaggg cttcggtcgc ccgttgatgc gaggtgtttc ccgagttgag ccctggcgta
      601 tcgatgatcc agaagtgttt cagcagctcg atcggcagca cgatctcgat ctcgcgcacc
      661 tgtcgcaagg cgaagggaag ctgcccgcgc gggtcgacaa gaggactttc gctgccggca
      721 actgggacgg atgagtgtcc gaacagcccc ggcaactgct cgatcggcag atactgtcgt
      781 tgcccgtcgt tggcaatcag cacggcaagc gggtaatcgc cgtaacgcag ggtgcagggc
      841 aggggagtgg tcggcagatt tccccgtggc agcgcgtttg ttccgagcag ggcgttgatc
      901 aaggtcgatt tgccggcatt gaactcgccc atcacggcca gcaacaccgg tagacccgca
      961 tcatgaacca gctggtcgaa tgcctcgaga gcggtgtcgg cagaacgaga agcggtgatg
     1021 gcggctcgca ggtcttttga caacagttcc tgcgggaatc ccttttccag gtgctgttgc
     1081 ttaaccgggt caggtggcga cccggctgcg agtcgttctt cgcgcgggaa ctgaaagagg
     1141 tcagcgatgg cagaggcgac ctggcgcgac cacgacgagg ctcgggagtg cagccaggtg
     1201 tttcgacacc agcgttgcag gcgcgctcgt cgcttcatgt cctgccgttc ccggaggcag
     1261 gcggcgatcg tgtgtcgact tgttgggctg agacgactct ctgattcttc ggggttggtc
     1321 tcgagcgccc acacctgcca ccagtcgtca cttcgccatg tgcgagtcga ggcggtggtt
     1381 gagttcagcc acgtgcgtac tggttctttt ctgccggcac ccagcaacaa ctcggtcgcc
     1441 cagaaacggt ctgaagggtt cgtcagatca gcgaaacgtt tttccacata ttcggcgatg
     1501 gctggctcac gcccgagcca gatggccaga cgacagagag gatacaaagg tctgaagccc
     1561 gttgtgccgc tgatcagctc gtacgcccca agataatctc cccggcagca gagcagggcg
     1621 gcttccacac gtatcgattc gaggggaatc cagtcctggt tttggcacgc cgattgccag
     1681 gcggcttctg ccgtatcggc ctgcccgcat tcgagaagct gccagatccg caacagttcc
     1741 tggaacgtcg gggtcagctg aaaggcgagc gctgtcgttg actcgagtgg aaaggcagac
     1801 gtgacgccga aatcctcggc cggggagccc cagaacccct gactttcacg tgacgtgaag
     1861 ggttcccgcg acgggttcgc ttcaccccac cgaccggaat gagccagcgc cagagaccga
     1921 atgcgttgcc acg
//
LOCUS       DAORHD010001042         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76301_length_1930_cov_3.638400, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001042 DAORHD010000000
VERSION     DAORHD010001042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76301_length_1930_cov_3.638400"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..>1930
                     /locus_tag="PKO06_18420"
     CDS             117..>1930
                     /locus_tag="PKO06_18420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71686.1"
                     /translation="MIPFSRWLTLVLLIWGIPAFLFVTGIGVYQTATDQRKIIWQHQQ
                     VEKRLLEFSQRCDFSSFFELRCHDLFARLASQTTAELRFREVEAFERQYPPGLVRCFL
                     FGRDTMLEWPHQHHAPEAAQQFFTLAKQSWKKHLVIDAEMSDRLQRFLANPEGTLRIL
                     QGRPGRMLSIGTPEASFGWYQFLPETATRSFSGMLILFNGRALPDGFLPRHFAAELSD
                     SIVQIGFRSNKGSTVIPSSISSELFHKLLRRFDLVPEDRFHVGSIIGGIQRFDGSTLI
                     IGVAPRLAATHRLLLVLLIAYAFLSFWLLSRSYRVLVLQEELRLSLPRKTFLFMLLGL
                     GFPLLISGMLSSAFLEEKRDTILQDLRDETVQELRQIDQGFDLFLTRRLQYYRKLSSW
                     LHHHIDQPALMQETLSRIYRQNDIDALYMVSSNSQHLGFFRGLPIEIRRILSEPEEVR
                     QQMFESAVERQYMPTFGESVAIAKGRFTASKANDYEQMLLRLISHAAKTAIDHYNEQH
                     GFETGPTTSLQQLAMDGLMDDEGQQYVEAVRSQLSGLIPMSSENDFGLNYMEVIPDQT
                     GRGGYFLILFHNGNALEHDYLRSLQKHSRRLAETFAVSL"
BASE COUNT          385 a          582 c          499 g          464 t
ORIGIN      
        1 cgcgggcgcg atcagccgac gaccgagatc gggccctggc tgtttcaacg tgcggatcgc
       61 cactttgcct cgatcgccct tcccgatcag acgattctgg tggtggatac ccctctatga
      121 ttcccttttc cagatggctc acactcgtac tcctcatctg gggaattccc gcatttctgt
      181 tcgtcaccgg aattggtgtg taccagaccg caacagacca gaggaagatc atatggcagc
      241 atcagcaggt agagaagaga cttctggagt tttcacagcg atgcgatttc tcgagtttct
      301 tcgagctgcg ctgtcacgat ctgttcgccc ggctcgcctc gcagaccact gccgaactgc
      361 gattccggga agtcgaggcc ttcgagcgcc agtacccgcc cggcctggtc agatgctttc
      421 tgttcgggcg cgacaccatg ctcgaatggc cgcaccagca tcacgctccc gaggctgcgc
      481 agcaattctt caccctggca aaacagtcat ggaaaaagca tctggtcatc gatgccgaga
      541 tgtcggatcg cctgcagcgg tttctggcaa atcccgaggg aacgctgcgc attcttcagg
      601 ggcgtccggg gcgaatgtta tccatcggca ctcccgaagc ctctttcggg tggtatcaat
      661 tcctgcccga aaccgcgacc cgctcatttt caggaatgct gattctgttc aacggacggg
      721 cccttcccga tgggtttctg ccgaggcact tcgccgccga actttctgac tcgatcgtac
      781 agattggatt tcgatcaaac aaaggatcga cagtcattcc gagcagtatc tcttctgagc
      841 tctttcacaa actgctacgc cgtttcgatc tcgtgcccga agatcgcttt cacgtcggca
      901 gcatcatcgg gggtattcaa cgtttcgacg gttcgactct gatcatcggc gtcgcacctc
      961 gcctcgccgc cacgcatcgt cttcttctgg ttcttctcat cgcctatgcg ttcctcagct
     1021 tctggcttct ctcacgcagt tatcgcgttc tggttctgca ggaagaacta cgtctgtcgc
     1081 ttccgcgcaa aacattcctc ttcatgctgc tcgggctcgg atttcctttg ttgatctccg
     1141 gcatgctctc ttctgcattt ctcgaagaga agcgggatac cattcttcag gatcttcgcg
     1201 atgaaacagt tcaggagctc cgccagatcg atcagggctt cgatctcttc ctcacgcggc
     1261 gactgcagta ttaccggaaa ctgagttcgt ggctgcacca tcatatcgat cagccagccc
     1321 tgatgcagga aacgttgtcg cggatctacc ggcagaatga cattgacgca ttgtatatgg
     1381 tctcttcgaa ttcacagcat ctcggctttt tccggggatt gcccatcgaa atcaggcgca
     1441 ttctgagcga gcccgaggag gtgcggcagc agatgttcga atccgcagtc gagcgtcagt
     1501 acatgcccac cttcggcgaa tcggtcgcta tcgccaaggg tcgtttcacc gcctcgaagg
     1561 cgaatgacta cgaacaaatg ctgctgcgcc tgatctctca tgccgcgaaa accgcgatcg
     1621 accactacaa cgagcaacat ggattcgaga ccggcccgac cacctcgttg cagcaactgg
     1681 ccatggacgg cctgatggac gatgaggggc aacaatacgt tgaagccgtg cgcagccagc
     1741 tttcagggct cattccgatg agctcggaaa acgacttcgg gcttaactac atggaggtta
     1801 ttcccgacca gaccggccgg ggcggctatt ttctcatcct gtttcacaat ggcaacgctc
     1861 tggagcacga ctacctgcgg tctttgcaga aacattcccg tcgtcttgcg gagacgttcg
     1921 ccgttagtct
//
LOCUS       DAORHD010001043         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76517_length_1927_cov_2.733974, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001043 DAORHD010000000
VERSION     DAORHD010001043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76517_length_1927_cov_2.733974"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..542
                     /locus_tag="PKO06_18425"
     CDS             120..542
                     /locus_tag="PKO06_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV71687.1"
                     /translation="MSTRAFFLGLLALLVLFTATVHADQDVRKNGSLWARIEDDGTLR
                     LNGSLVGKFESDGTVRKNGSLEGKVESDGTIRHNGSLVGRVEEDGTLRRNGSIIGRIE
                     SDGTIRRNGSIWGRAEHAASFDQKRAIAALLFFFTDEF"
     gene            542..1408
                     /locus_tag="PKO06_18430"
     CDS             542..1408
                     /locus_tag="PKO06_18430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV71688.1"
                     /translation="MRATGRALRQTFCVLICFCLFFDLAAIVLARGNEDAVCRVEINK
                     ANPNAPEYFRVTNKTAGDLTINFLLPTANNVTATPTLPRTWVLQPRASESLFSIHPAN
                     SRRAWRYEYRFFWRNGRPNARHDDSQRYRLPFPSGQRFRVIQGNNGSFSHFGEYQYAV
                     DFDMPEGSIICAARGGVVVRAEDSFSGRGLTDEYRNRTNVILIQHDDGTIGEYAHLRK
                     GGKLVHMGDTVSTGQKLGYSGDVGYSDGAHLHFGVWKPIDGKTIESIRVTFTDGVNMY
                     NELREQVTYTAH"
     gene            1437..>1927
                     /locus_tag="PKO06_18435"
     CDS             1437..>1927
                     /locus_tag="PKO06_18435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71689.1"
                     /translation="MNWIRRHPFITLFGLLGLLTLFLGFELLSTLEDLKLMMDVMNGK
                     VSLPSLTTARGKSRPPRYSLKLLATFSIPLSLADEAGTKAGGTWDHSRPLFPSNSSAG
                     DVYAKIASETPLAIKIAEHLTQSLNHLPTCASLPALLDFGASTANYREVRTHARFLVF
                     LAR"
BASE COUNT          399 a          576 c          524 g          428 t
ORIGIN      
        1 caatccctga aagatcatgg tttcttcatc ccccatcggc aaatctgata acgggcatgc
       61 cactcgaacc gatgatatca tactggtata cgcatgcatt tttcccattg gaggaaccga
      121 tgagcacacg cgcatttttt ctggggttgc tggcacttct cgtcctgttc accgcgaccg
      181 ttcatgccga tcaggacgtg cgcaaaaacg gcagtctgtg ggcccgcatc gaggatgacg
      241 gcacgctgcg tctcaacggc agcctggtcg gcaaattcga gagcgatggc accgtgcgca
      301 agaacggcag cctcgagggg aaagtcgaga gcgacggcac gattcgccac aacggctcgc
      361 tggtcggtcg cgtggaagaa gatggcacgt tgcggcgcaa cggcagcatc atcggccgca
      421 tcgagtctga cggaacgatt cgacgtaatg gctcgatctg gggtcgggca gagcatgcgg
      481 catcgttcga tcaaaaacgt gccatcgcgg cgttgctgtt cttcttcacg gacgagttct
      541 gatgagagcc actggtcgcg ccctgcgcca gacgttctgc gtcctcatct gcttctgcct
      601 gttcttcgac cttgcggcga tcgtcctggc tcgtggcaac gaagatgcgg tctgccgcgt
      661 cgagatcaat aaggcgaatc ctaacgcacc cgaatatttt cgggtgacca acaaaaccgc
      721 gggcgatctg accatcaact ttctgctgcc aaccgcgaac aatgtcaccg ccacccccac
      781 tctgccgcgc acctgggtgc tccaaccgcg cgcctctgaa tcgctctttt ccattcatcc
      841 ggcaaattca cgccgtgcgt ggcgttacga gtatcgcttc ttctggcgca atggtcgacc
      901 gaatgcgcgt cacgacgatt cacagcgcta ccgactgccc tttccgtcag gccagcggtt
      961 tcgcgtcatt cagggaaaca acggctcgtt ttcgcatttc ggcgagtatc agtatgctgt
     1021 cgacttcgac atgcccgaag ggtcgatcat ctgcgcggct cgtggcggag tcgtcgtgcg
     1081 cgccgaagat tcgttcagcg gtcgcggtct cacggatgag tatcgcaatc ggaccaacgt
     1141 cattctgatt caacacgatg acggaacgat cggcgagtat gcgcacctgc gcaagggcgg
     1201 caagctggta cacatgggtg ataccgtcag taccggccag aaactcggct attccggtga
     1261 tgttggctat tctgacgggg ctcatctgca ttttggagtc tggaaaccaa tcgatggcaa
     1321 aaccatcgaa tcaattcgcg tcaccttcac ggacggcgtc aatatgtaca atgaattacg
     1381 ggagcaggtg acgtataccg ctcactgaat caggcttccc gctgaaagga accactatga
     1441 actggattcg ccggcatccg tttattaccc tgtttggcct tctgggactg ctcactctgt
     1501 ttctcggttt cgagcttctg agcactctcg aagatttgaa gctgatgatg gacgtcatga
     1561 atggaaaagt gtcgctgcca tcactgacga ctgctcgagg caagagtcga ccgccgcggt
     1621 attctctcaa actccttgcc accttcagta tccccctgtc tctcgctgat gaagcgggca
     1681 ccaaagcggg gggcacctgg gaccacagcc gaccactctt cccgagcaac agttcggccg
     1741 gcgacgtgta tgcgaaaatc gccagcgaaa cccctttggc gatcaagatc gcggagcatc
     1801 tgacccagtc actgaatcat cttcccacct gtgccagtct gccggccctg ctggacttcg
     1861 gcgcctcaac ggcgaactat cgtgaagtcc gcactcatgc tcgctttctg gtttttctcg
     1921 cgcgcca
//
LOCUS       DAORHD010001044         1923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76724_length_1923_cov_3.375268, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001044 DAORHD010000000
VERSION     DAORHD010001044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76724_length_1923_cov_3.375268"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PKO06_18440"
     CDS             complement(<1..1312)
                     /locus_tag="PKO06_18440"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV71690.1"
                     /translation="MIVFRSMKMKVFLLVLALLCLFPVLAFAEGMEYVEIVGNDGQTR
                     RFSRLVMGTDHLIQAGWVEDGQPEPKKEQVFAVLDEAARLGINLFDTAPIYVGGVENA
                     LGEWRASRSAQIASDGFYVQPSLNPDRKLYALSKGGFPYDLYWLKELPAGCHSAETIA
                     ELKSRGILASDAVDWRNRKWPLKDVPPGTYLSHLYCEQKLMIERIAAELVHTCANLKC
                     EIDVYLMHRDDGDAIGFEPVKREQTPVRRILEAVSAPEISKNFKVLGWSNWKTPRVEE
                     SLRLAAKEPKLTPPAINSPYFSLFEMSDRTIHALGVQVTHAEMMNPDFQKGIRIMPYS
                     PLGGFSILDKPAPAWENAKKAALKKHQDGDAYWRNVYPAIFTPENEARWHRAVDFLDR
                     FNKRHTTTYTIDQLLNAYVLAHPRTDLLAIGAITVEQVRRTAKAL"
     gene            complement(1383..1586)
                     /locus_tag="PKO06_18445"
     CDS             complement(1383..1586)
                     /locus_tag="PKO06_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71691.1"
                     /translation="MKPYIGVGGVIVFLPSLVCLFLFGKSRGMWEAFQIGFNVTFAVL
                     GIAIVTFGVSVAVKTVSALRSKE"
     gene            complement(1695..>1923)
                     /locus_tag="PKO06_18450"
     CDS             complement(1695..>1923)
                     /locus_tag="PKO06_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71692.1"
                     /translation="VEWLRSLSYLKAAVLCPDTQFSDPHKRFIKARLDTLAANLGKHE
                     ISSWLLPLSEYRGQHMSTTGRISCPHHGTVE"
BASE COUNT          412 a          579 c          539 g          393 t
ORIGIN      
        1 cgagggcttt ggcggtacgg cgcacctgtt cgaccgttat cgcaccgatc gccagcaggt
       61 cggtgcgcgg gtgcgcgagc acataggcgt tcaacagctg atcgatggta taggtggtgg
      121 tgtgccgctt gttgaaccgg tcgaggaaat ccaccgcgcg gtgccagcgc gcctcgtttt
      181 cgggggtgaa gatcgccggg tagacgtttc gccagtaggc atcgccgtcc tgatgtttct
      241 tcaacgccgc cttcttcgcg ttttcccacg ccggagccgg tttgtccaga atgctgaagc
      301 ccccaagcgg ggaatagggc atgatcctga tgcctttctg aaaatccggg ttcatcatct
      361 cggcatgggt cacctgaacg ccgagcgcgt gaatggtacg atcactcatc tcgaacagag
      421 aaaagtacgg gctgttgatc gccggaggtg tgagtttggg ctcttttgcc gcgaggcgaa
      481 gcgactcttc gacgcgcgga gtcttccagt tcgaccaacc caacaccttg aagttctttg
      541 aaatctcggg cgcgctgacg gcttcgagaa tccgacggac gggcgtctgt tcgcgtttga
      601 cgggctcgaa cccgatcgcg tctccgtcgt cgcggtgcat cagatagaca tcgatctcac
      661 acttcagatt cgcgcaggtg tgaaccagct cggccgcgat gcgctcgatc atcagtttct
      721 gttcgcagta caaatgactc aggtaggttc ccggcggtac gtccttgagg ggccatttcc
      781 gattgcgcca gtcgacggcg tcagacgcga gaattccccg actcttgagt tcggcaatcg
      841 tttcggccga atggcagccg gccggcaact ctttcaacca gtacaaatca tacggaaacc
      901 cgcccttgga gagcgcgtac agctttcggt cggggttcag cgaaggctga acgtagaacc
      961 cgtcgctcgc aatctgcgcc gagcggctcg cccgccactc gccgagggcg ttttcgacgc
     1021 caccgacata gatcggcgcg gtatcgaaga ggttgatgcc caggcgggcg gcttcatcga
     1081 gcaccgcgaa cacctgttcc ttcttcggct ccggttgtcc gtcctcgacc caccccgctt
     1141 gaatcaggtg atcggtgccc atcaccagcc gcgaaaaccg tctggtctgg ccatcgtttc
     1201 cgacaatctc gacgtactcc atgccctcgg cgaaagccag aaccggaaac aggcacagca
     1261 gcgcgagaac cagcagaaaa accttcatct tcatcgatct gaacacgatc atggcatcac
     1321 tctcctgtta ttcgtactcc ttcatcgctc gcgaacacgt gatgaacaag ggttcaagcc
     1381 catcactctt tgcttctcaa ggcagagacc gttttgacgg caacagacac tccgaaggtc
     1441 acaatcgcta ttcccagcac agcgaaggtc acgttgaaac cgatctgaaa ggcttcccac
     1501 atgccccgac tcttgccgaa caagaagaga cacaccagcg acggcagaaa aacgatgacg
     1561 cccccgaccc caatataggg cttcatgaac atggcaggca cctcccggat tcaacactga
     1621 aaaatcaacc acgaactgct gtttctctgg tctggccacc ccaaccggtc gcgaccgatc
     1681 gcgcgcggtc tcgatcattc gacggtgcca tgatgcggac aggagattct gccggtcgtt
     1741 gacatgtgct ggccgcgata ctccgagagc ggaagcaacc agctggaaat ctcatgcttg
     1801 ccgagattcg ctgcgagggt atccagacgc gccttgataa aacgcttgtg cggatctgag
     1861 aattgcgtat cggggcacag aaccgcggct ttgagataag agagagaacg cagccactcg
     1921 acg
//
LOCUS       DAORHD010001045         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76784_length_1922_cov_3.775040, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001045 DAORHD010000000
VERSION     DAORHD010001045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76784_length_1922_cov_3.775040"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PKO06_18455"
     CDS             complement(<1..1615)
                     /locus_tag="PKO06_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71693.1"
                     /translation="MSGSYFQEDEIVGKIYDARLIRRIFGYLGPYRWRAALAVLFVIC
                     SMSLFIVNPWLLGKIVDQGIKKADLLLIRNLSLFYLGIEILVYLSTCAGSYLLQWVGQ
                     NVMYDLRSQLFSHLQRLPIAFFDRNPVGRLVTRTTNDVAAMSELFSSGIVVVVGDVVL
                     IVGIALALLWQHLFLGVMTLITIPLLIVAAWYFQSRIRDAYRQVRVRIARVNASLSEG
                     ISGVKVVQIFNREALQIERFETLNVSHRESQLQSLFYHAVFTPVVTVINAITIVIILL
                     IGGSMVQRGEVSLGTLVSFLAYAQHFFFPIRDISEKVSIFQSAMASAERVFGLLEEAE
                     EPVTTVSGTMTASPQATADTEGSALPAIKGGITFENVSFSYVPGEPVLKDLSFAIEPC
                     QSVALVGHTGAGKTTIASLLNRFYDIQQGRILIDGLDIRSFDRRNLRRQIALIQQDVF
                     IFSGNVLENIRLWNPTYDEAWVAQVAAEVHAHDFISRLPDGYKSEIYERGTNLSAGQR
                     QLLSFARALCTRPRILILDEATSSVDSDTE"
     gene            complement(1612..>1922)
                     /locus_tag="PKO06_18460"
     CDS             complement(1612..>1922)
                     /locus_tag="PKO06_18460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV71694.1"
                     /translation="TLARAIARDPRVLLLDDCVSAVDTDTEDRILAELRQVMRDRTSI
                     IVSHRLVSVQQADLILFFDQGRVVERGTHAELLAAGGRYARMWEKQRIRSELESGVGA
                     "
BASE COUNT          431 a          534 c          596 g          361 t
ORIGIN      
        1 tctcggtgtc gctgtcgacg gacgacgtcg cttcgtcgag aatgagaatg cgcgggcggg
       61 tgcagagcgc ccgggcgaat gacagaagtt gtcgttgtcc ggccgagaga ttggtgccgc
      121 gttcgtagat ttctgacttg tacccatcgg gaaggcgtga gatgaagtcg tgggcatgca
      181 cctcggcggc gacctgcgct acccaggctt catcataggt cgggttccag aggcggatgt
      241 tctcgagcac gttgccggaa aatatgaaga catcctgctg aatcagggcg atctgccgtc
      301 tcagattccg gcgatcgaac gaccgaatat ccaggccgtc aatgagaatg cgaccctgct
      361 ggatgtcata gaatcggttg agcagcgatg cgatggtggt cttgcccgcc ccggtgtgtc
      421 cgaccagggc cacgctctgg cacggttcaa tggcgaatga gaggtctttc agcaccggtt
      481 cgcccggaac atacgagaac gacacgtttt caaacgtgat gccgcccttg atggccggca
      541 gtgccgagcc ctccgtgtcg gccgtcgcct gcggtgaggc ggtcatcgtt ccggatacgg
      601 tagtgacggg ctcttccgct tcttccagca gtccgaatac gcgctcggct gaagccatcg
      661 cactctggaa gatgctgacc ttttcgctga tgtcgcgaat gggaaagaag aagtgctggg
      721 cataggccag gaaactgacc agagtgccga gtgagacttc gccgcgctgc accatactgc
      781 cgccgatcaa tagaatgatg acaatggtga tggcattgat gacggtcacg acgggagtga
      841 acaccgcatg atagaacaga gactggagct gggattcccg gtgcgacacg ttgagtgttt
      901 caaaacgctc gatctgaagc gcttcgcgat tgaagatctg caccaccttc accccggaga
      961 tgccctctga gagcgaggca ttgacgcggg cgatgcgaac gcggacctgc cggtaggcgt
     1021 cgcgaatgcg gctctgaaaa taccaggcgg ctacgatcag cagcggaatc gtgatcaagg
     1081 tcatgacgcc gagaaacaga tgctgccaga gcagggccag cgcaatgccg acgatcagca
     1141 cgacatcgcc cacgacgacc acgattccgg aagagaacaa ctcggacatg gcggccacgt
     1201 cgttggtggt tctggtgacc aggcgtccga ccgggttccg gtcaaagaac gcgatcggca
     1261 gtcgctgcag atgagaaaac aactgcgaac gcaggtcata catgacgttc tgaccgaccc
     1321 actgcagcag atacgaaccg gcgcaggtcg agaggtagac cagaatctcg atgccgagat
     1381 agaacaggga cagatttcga atcagcaaca gatctgcttt cttgatgccc tggtcgacga
     1441 ttttgcccag cagccacgga ttgacgatga acagactcat gctgcagatg acgaacagca
     1501 cggcaagcgc ggcgcgccag cgataggggc cgagatagcc gaaaatgcgg cgaatgagac
     1561 gggcgtcgta gattttgccg acaatctcgt cttcttgaaa gtacgagccg ctcatgcgcc
     1621 tacccctgat tcaagctcac ttctgatccg ttgcttttcc cacatacggg cgtaacgacc
     1681 gccggcggcg agcagctcgg cgtgggtgcc ccgttcgacg acgcgcccct gatcgaaaaa
     1741 cagaatcagg tctgcctgct gtacgctgac caatcggtgc gaaacgatga tgctggtgcg
     1801 atcgcgcatg acctggcgca gctcggccag aatacgatct tcggtgtcgg tgtcgacggc
     1861 actgacgcag tcgtcgagca gcagcacccg aggatcacgt gcgattgctc gtgccagcgt
     1921 ca
//
LOCUS       DAORHD010001046         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76846_length_1921_cov_4.490890, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001046 DAORHD010000000
VERSION     DAORHD010001046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76846_length_1921_cov_4.490890"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKO06_18465"
     CDS             complement(<1..188)
                     /locus_tag="PKO06_18465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71695.1"
                     /translation="MGIPSPGASSPLLLPHFPELVILLKPQGPVIDQLPPWLAEIVAP
                     PTAVSSPEFFQILAQRLPG"
     gene            412..972
                     /locus_tag="PKO06_18470"
     CDS             412..972
                     /locus_tag="PKO06_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71696.1"
                     /translation="MKRKNPETYQRNLQFLSGERLETILSTLQKFFPADKELIQTLQR
                     KDLDMMLMRIMRSEVVHLQGEGERLVQKGRNVLMQFGFIPPVAFFFAFNGAELFDAMI
                     ERIHQDLRKRGRPQGLSDIWKRLSAPLMAGNETKTVFTKDLLFKIRQRLKNYLEKDRR
                     FVIDAKSPKKLYLRLTEWGDKPVDLD"
     gene            988..>1921
                     /locus_tag="PKO06_18475"
     CDS             988..>1921
                     /locus_tag="PKO06_18475"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV71697.1"
                     /translation="MRTLSPLRPRTGQVFVLGNWRWIVFFLIWGLCLGVVISGRPDSP
                     LWYLAILYLGAALFVTARKFYRTRAELLFLREFHHLSQTLDDIRALEVGNPFDPILAL
                     VTRLVGFDRAVLLLPERTDGQLRPVRSYGVPEATLALLPAVAPAEALVWRSLQRGEIL
                     LIDPTDPALAADPPLRELAAGSLTICLPIQRRQQNLGVLLLFHGDAEVPLSDDVLLQL
                     QILIDQIGISLHNLTLHQDLREKAQELELRDARMNKELELARLVQDGVLPWTSPTWPG
                     MTISAFFRPARFIGGDYYSYLDGCSHNRRDCVSKS"
BASE COUNT          394 a          523 c          566 g          438 t
ORIGIN      
        1 cccggaagtc gctgcgccag gatttggaag aactctgggg aagagaccgc ggttggtggg
       61 gcgacgattt ctgccagcca tggtggcagt tggtcgatga ccggcccctg gggtttaagc
      121 agaatgacca gttcgggaaa atgggggagc aacagcggcg acgaggcacc gggtgatgga
      181 atccccatga tttgatctcc gtcttgtgtt ggctgcgatg gtcagcggca ctccgcgtct
      241 gcttcagcat accgccggat gaaattctta tcaaccggca gagaaaaatg cttgaatcag
      301 cgaaggtttc cgattaggat aaataaaaat ttatctctca acggatcggc tcacatggcg
      361 agtgcctcaa aattcgaatt tattctgagc tttgagaagg tgctcgggga aatgaagcgc
      421 aagaaccccg aaacctatca gaggaacctg cagtttctca gcggtgagcg tctggaaacg
      481 attctttcga ctctgcagaa gtttttcccc gccgacaaag agctgattca gactctgcag
      541 cgcaaagacc tcgacatgat gttgatgcgt atcatgcgct cggaagttgt acatctgcag
      601 ggagaaggag aacgactggt acagaaaggg cggaatgtcc tgatgcagtt cgggtttatt
      661 ccgccggtcg ccttcttctt tgcgttcaac ggtgccgagt tgttcgatgc catgatcgag
      721 cgcatccacc aggacctgcg caagcgcggc cggccccagg gtctctcaga catctggaaa
      781 cgtctgtcag cgcctcttat ggccgggaat gaaacgaaaa ccgtgttcac caaagatctg
      841 ctgttcaaga ttcgccagcg tctgaagaac tacctcgaaa aagatcgtcg attcgtcatc
      901 gacgcgaaaa gtcccaagaa gctgtatctg cgtctgacag agtggggtga caagcccgtc
      961 gatctcgact gacggcgcac gaatcacatg cgtaccctgt caccgctgcg accacggaca
     1021 gggcaggtgt ttgtcctggg aaactggcgc tggatcgtct tctttctcat ctgggggttg
     1081 tgtctgggcg tggtcatcag tggtcgcccg gattcgccgc tctggtatct cgcgattctg
     1141 tatctgggcg cggcgttgtt cgtcaccgct cgcaaattct atcgcacacg tgccgaactg
     1201 ctgtttcttc gcgagtttca ccatctgtca cagacgctcg atgatattcg tgcgctcgag
     1261 gtgggcaacc cgtttgatcc gattctcgcc ctggtcacgc gtctcgtcgg ctttgatcgc
     1321 gcggttctgc tgttaccaga acggacggat ggtcagttgc gcccggtacg cagttatggc
     1381 gtgcccgagg cgacgctggc attgttgcca gccgtggccc cggccgaagc gctggtctgg
     1441 cgttcattgc agcgcgggga gattctgctg attgatccga cagacccggc cctggcggcc
     1501 gacccgccgt tgcgcgagct ggcggccggc agtctcacga tctgtctgcc gattcagcgt
     1561 cggcaacaga accttggagt gctgctgctg tttcacggcg atgccgaagt gcccctctct
     1621 gatgacgtgc tgctgcagct gcagattctc attgatcaga tcggcatcag tctgcacaac
     1681 ctgaccctgc accaggatct gcgggaaaaa gctcaggaac tcgagctacg ggacgcgcgc
     1741 atgaacaagg aattggagtt agcgcgactt gtacaagacg gggttctgcc ctggacttcc
     1801 ccaacctggc ctggaatgac catctcggcg ttcttccgtc cggcgcggtt catcggcgga
     1861 gactactaca gctatctcga tggctgctca cataatcgtc gcgattgtgt cagcaagtcg
     1921 t
//
LOCUS       DAORHD010001047         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76869_length_1921_cov_2.984459, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001047 DAORHD010000000
VERSION     DAORHD010001047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76869_length_1921_cov_2.984459"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKO06_18480"
     CDS             <1..452
                     /locus_tag="PKO06_18480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71698.1"
                     /translation="KLAASSAGKVATEHLDGAEDLTMRIHEALTACIAGDFALGLQLA
                     QQILAEHPEDLQARLIRGQAYAGLGQQSDAIAEFMGIAHSHPEEPDAFFHLGTLFLSL
                     NELEKALKYFQSVYRLDPTYSLVKENIANIERKILESKPHKPGAKGS"
     gene            449..>1921
                     /locus_tag="PKO06_18485"
     CDS             449..>1921
                     /locus_tag="PKO06_18485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV71699.1"
                     /translation="MTKTSLRFGIRFKLILIICAMGLLPLTFLGYQAYNEQRRVLMEE
                     ISRGHKELSNMLSHGIFENLEHTRQLLNAIVELDVIKQLNPVVAQDFFKSLLRHYPIF
                     KLLYLVDGNRQIIASTDPGAALPEDWLFSHAVKRSYQGALSEVAQTSEGNPYMTLETV
                     IKSPEKGVIGVLVSEVDLLYIRTLLINALKRSKSQGLVLDETGTVIARSSPEVKTFTV
                     SAAEVIDQDINDVRDVSGTRYLVTAVSLKKFNFYQAPNWTIILQIPEKVAFQAADELK
                     QRVQTLGALTGIVALILAFLLANHLVGPLINLITGARFISRGAFDHEIHATSKDEIGE
                     LARTFNEMRVNLKMTKADLDYRIIQLSTLYEVGKAINSELDFVKLQNMILETVARVLR
                     AEKASLMLLDETEKILTIGTAFGLSEEVAKSTRLEVGEAVAGWVLESGKALFVQDVES
                     DHAFLALKKSNVSRGTLMSVPLRAKDKILGVLNVSRSEPNS"
BASE COUNT          432 a          580 c          518 g          391 t
ORIGIN      
        1 acaaactggc ggcctcgagt gccggaaagg tggcaaccga gcatctggac ggggctgaag
       61 acctgacgat gcgcattcac gaggcactca ccgcctgcat tgccggcgac ttcgctctcg
      121 ggctgcagct cgcgcagcag attctggccg aacacccgga agacctgcag gccaggctga
      181 ttcgcgggca agcctacgcc gggctcggcc agcagtccga tgccatcgct gaattcatgg
      241 gcattgcgca cagccacccg gaagaacccg atgccttctt ccatctcggc acgctgtttc
      301 tcagtctgaa cgagctcgaa aaagcgctga agtatttcca gagcgtctat cgtctcgacc
      361 cgacctactc gctggtaaaa gaaaacattg ccaacatcga gcggaaaatt ctcgaaagca
      421 agcctcataa accaggcgcc aaaggctcat gaccaaaaca tccctgaggt tcggtatccg
      481 tttcaagctg attctcatca tctgcgcgat gggtctgctt cccctgacgt ttcttggcta
      541 ccaggcatac aacgagcagc ggcgcgtcct catggaggag atctcccgcg ggcacaaaga
      601 gttgtcgaac atgctctcgc acggaatctt cgagaatctc gaacacactc gccaactgct
      661 gaacgcgatc gtcgaactcg atgtcatcaa gcagctcaac ccagtggtcg cgcaggattt
      721 cttcaagtct ctgctgcggc actatccgat cttcaagctg ctctatctcg tggatggcaa
      781 tcgccagatc attgcttcga ccgacccggg cgcggctctg cccgaggact ggctcttctc
      841 gcacgctgtc aagcgcagct accagggggc gttgtccgag gtcgcccaga cttctgaagg
      901 caatccgtat atgactctcg aaaccgtgat caagagcccg gaaaagggag tcatcggagt
      961 cctggtctcg gaagtcgatc tgctctatat ccgcacactg ctgatcaacg cgctcaagcg
     1021 ttcgaagtcg caggggctgg ttctcgatga aaccggtacc gtgattgccc gttcttctcc
     1081 tgaagtgaag acgttcaccg tctcggccgc ggaagtgatc gatcaggata tcaatgacgt
     1141 ccgcgacgtc agcggcacac gctatctcgt cacggcggtc tcgctcaaga agttcaattt
     1201 ctatcaggcc ccgaactgga caatcattct tcagattccc gagaaggtgg cttttcaggc
     1261 cgccgacgag ctaaagcaac gagtacaaac actcggcgca ctcaccggca tcgtcgccct
     1321 gatactcgcc tttctgctgg ccaaccatct tgtcggcccg ctgatcaacc tgatcaccgg
     1381 cgcccggttc atcagccgcg gcgcgttcga ccacgagatt cacgcgactt cgaaggacga
     1441 gatcggcgaa ctcgcgcgca cgttcaacga gatgcgggtc aacctcaaga tgaccaaagc
     1501 tgatctcgat taccgcatca tccagctctc gactctgtat gaggtcggca aggccatcaa
     1561 ttcggaactc gatttcgtca aactgcagaa catgattctc gagaccgtcg cccgggtgtt
     1621 gcgtgccgag aaggcatcgc tgatgctgct cgatgagacc gaaaagattc tgacgatcgg
     1681 gaccgccttc gggctgtcgg aagaggtcgc caagtcgacc cgtctcgaag tcggcgaagc
     1741 cgtcgccggg tgggtgctcg aatcgggtaa agccctgttt gtacaagatg tcgaaagcga
     1801 tcacgctttt ctcgcgctga aaaagagcaa tgtgagtcgt ggcacgctga tgtccgtacc
     1861 tctgcgggcg aaagacaaga ttctcggcgt gctcaatgtc agccgaagcg aaccaaattc
     1921 c
//
LOCUS       DAORHD010001048         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_76972_length_1919_cov_4.631974, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001048 DAORHD010000000
VERSION     DAORHD010001048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76972_length_1919_cov_4.631974"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1919)
                     /locus_tag="PKO06_18490"
     CDS             complement(<1..>1919)
                     /locus_tag="PKO06_18490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71700.1"
                     /translation="RVYATIDTDRSVICAIRSLSQNAHVLVFEYQPPHSTAPFLTPGV
                     ISALSITFLLLLLAWARAFTLMPSWNIPLQAKFRRIIFVILLFPFGGMLFMYANTEQQ
                     KRESHLSQVESTLEERLESVETRAELSVYQRLFDITKGINSYDWRVRPSQKEMTELFN
                     RHEAQGLEELFVLHHSSPHLLSQKAQTAGRHGHIRRMVSLIFRQFQDYTGLYVNQDST
                     PSNSADELQSRFLMEGMQDLFGGESLYQLALHQNHLLSFSLFEEMTWICIQTLRDSSH
                     RPLAGFLLTLSRKDLQRQILGEMVLDATSFHSLPSLTFAHTLERSGLHFMPEAAASHP
                     DLHHLLTLVARTGITHRATIQAQGRTYLALARRLRFFDYAAVAIEPLPEVSAFSGNTV
                     LFLFLSAIPLLGFLLGNLRFRHRYLEPILQTEQAVHELTHGNFSVSFPSEAHDEIGEL
                     GRAFTNMSASLKEKEFLQRFLSDFTMDLVRNPTQIKASRLQATILFADIRGFTTLSES
                     CPPEELVDTLNEYFSRMEAVIEAHGGSIDKFIGDAIMAVFLPRLDAEPSAVRAAKAGL
                     EMQREVSSLSEARQALNRFPIRIGVGLATGEVLLGLIGSDSGRRDFAVTGPPVSLAAT
                     MEKRSKDGISSRVVV"
BASE COUNT          431 a          500 c          592 g          396 t
ORIGIN      
        1 cacgacgacc cgactcgaga ttccgtcctt tgagcgtttt tccatggtgg cggccagact
       61 gacaggcggc ccggtgaccg cgaaatcgcg tcgcccggag tcgctgccaa taagcccgag
      121 cagcacttct cccgttgcca gaccgacgcc gatccgaatg ggaaagcgat tcagagcctg
      181 tcgagcttcg gagaggctcg agacttcacg ctgcatctcg agacctgctt tggcagcccg
      241 aaccgcactg ggttccgcgt cgaggcgggg aagaaacact gccatgatcg catcgccgat
      301 gaacttgtcg atacttccgc cgtgcgcttc gatcaccgct tccatgcgag agaaatactc
      361 gttcagggtg tcgaccaact cctcgggtgg acaggattcc gagagggtcg tgaatccgcg
      421 aatgtccgcg aaaagaatgg ttgcctgcag acgcgaagcc ttgatctgtg tcgggttgcg
      481 aaccagatcc atcgtgaagt cagacaggaa ccgctgcagg aattcctttt ccttgagact
      541 ggcggacatg ttggtgaatg cgcggccgag ctcgccgatt tcgtcgtgcg cctcagaggg
      601 gaacgaaacc gagaagttcc cgtgcgtcag ttcgtgcacg gcttgctccg tttgcagaat
      661 cggttccagg tatctgtgcc ggaaacggag gtttcccaac agaaatccga gcaggggaat
      721 cgcgctgaga aacaggaaca acaccgtgtt cccggagaat gccgatactt caggcagcgg
      781 ctcgatggcc acagctgcgt agtcaaagaa acgcagtctg cgagccagag ccagataggt
      841 tcttccctga gcttgaatgg tggcacgatg cgtgattccc gttcgcgcga cgagggtcag
      901 caggtgatgc agatcagggt ggctggctgc cgcctctggc atgaagtgga gaccggaccg
      961 ttcgagagtg tgagcaaagg tcagcgacgg cagagaatgg aacgaggtcg catcaagaac
     1021 catctctccg agaatctgtc gttgcagatc cttgcgactc agcgtcagca ggaatccggc
     1081 gaggggtcga tgagaactgt ctctgagagt ttgaatgcag atccacgtca tttcttcgaa
     1141 gagggaaaac gagagcagat gattctggtg cagagccagc tggtacaggc tttcaccacc
     1201 gaagagatcc tgcatgcctt ccatgagaaa gcggctctgt aactcgtcgg cggagttgct
     1261 cggggttgag tcctgattga catacaaccc cgtgtaatcc tgaaactggc gaaagatcag
     1321 gctcaccatg cggcgaatgt gtccgtgacg tcccgcggtc tgggcttttt gagaaagcag
     1381 gtgcggtgag ctgtgatgca ggacgaacag ttcttcgaga ccctgtgcct catgacggtt
     1441 gaacagttcg gtcatctcct tctgtgaagg tcgaacgcgc caatcgtacg agttgatgcc
     1501 tttcgtgatg tcgaacaggc gctgatacac gcttagttcc gcgcgcgttt ccacgctctc
     1561 aagtctctcc tccagagttg attcgacctg actgagatgg ctctcgcgtt tctgctgctc
     1621 ggtgttggca tacatgaaca gcatgccgcc gaacggaaaa agcaggatca cgaagatgat
     1681 tcggcggaac tttgcctgaa gtggaatgtt ccaggagggc atgagagtaa acgcgcgagc
     1741 ccaggccagc agaagcagca gaaaggtgat gctgagtgcg ctgattactc ctggtgtcag
     1801 gaatggcgcc gtggaatgcg gcggttgata ctcgaaaacg aggacgtgag cattctggct
     1861 cagcgagcga atggcgcaga tgacggaacg gtcagtatcg atggtggcat agacccgtt
//
LOCUS       DAORHD010001049         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77240_length_1915_cov_3.538710, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001049 DAORHD010000000
VERSION     DAORHD010001049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77240_length_1915_cov_3.538710"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKO06_18495"
     CDS             complement(<1..240)
                     /locus_tag="PKO06_18495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV71701.1"
                     /translation="MPSVYPVGMAELRVGQEPDILAAYGVGSCVIVIMFDPRSKKGGL
                     AHVVLPDSSGISQERLNPRKFADTAVPLLYQTLTHA"
     gene            complement(243..860)
                     /locus_tag="PKO06_18500"
     CDS             complement(243..860)
                     /locus_tag="PKO06_18500"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HNV71702.1"
                     /translation="MSDFDVIRRDQLDALREIGNIGSGNAATALAQFLNRKIDMDVPQ
                     AKILPINQIPGLFGGPEARVLGIYLKVLGSITGRFMLMIPEVTAIRMLNILIPGCEIT
                     SGQQMGDMERSCMREIGNILAGSFLNALSGLTNVPVLCSLPTLAFDMAGALLDTVVGE
                     MIAYSDDVLMIETSFFDDNQDLRMHIFLMPDAESIQKLFELLGIK"
     gene            complement(951..1568)
                     /gene="pth"
                     /locus_tag="PKO06_18505"
     CDS             complement(951..1568)
                     /gene="pth"
                     /locus_tag="PKO06_18505"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV71703.1"
                     /translation="MIIAGLGNPGPEYDRTRHNLGFIAVDALTRHFRTQLSGHRCRAR
                     VGEFSFKGSRHLLVKPQTYMNLSGESLGPLLQEERATPAELIVIIDDVCLPVGKVRIK
                     PNGSAGGHNGLKSIIGSIGEGFWRIRIGVGSPDGNLVAHVLGPISQQEHDVLLDVVKD
                     IPQMVCLLMAGMGSRAMSVFNGKSYLPEPEPALVPSPPHPRAPLD"
     gene            1715..>1915
                     /locus_tag="PKO06_18510"
     CDS             1715..>1915
                     /locus_tag="PKO06_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71704.1"
                     /translation="MKQRIGFFVLVSLFVIACTAFAALEQQMNTLSPLREGDVLRIKV
                     MNNRSLVEIDQDVTVNANLDIDV"
BASE COUNT          438 a          510 c          565 g          402 t
ORIGIN      
        1 ggcgtgcgtc agcgtttgat acagcagcgg aacggccgtg tcagcaaact tgcgcggatt
       61 cagacgttcc tgagaaatgc cactggaatc gggcagcacc acgtgagcca gccccccctt
      121 ttttgaacgc ggatcaaaca taatgacgat cacacaggaa ccaaccccgt aagctgcgag
      181 gatatccggc tcctgtccga cccggagttc ggccattccg acgggataca cagagggcat
      241 gtttacttga ttccgaggag ttcgaaaagt ttctgaatgg attcggcgtc gggcatcagg
      301 aagatatgca tccgcagatc ttggttatca tcaaaaaacg aggtttcaat catcagcaca
      361 tcatcgctat aggcgatcat ttcgccaacg acggtgtcaa gcagcgctcc agccatgtcg
      421 aaggccagag tcggcaatga acacagaacg ggcacgttcg ttaagccgga aagagcgttc
      481 aggaaagaac cggcgagaat attgccgatc tcgcgcatac acgagcgctc catgtctccc
      541 atctgttgcc cggatgtaat ctcgcagcca gggatcagga tattgagcat gcgaatcgcg
      601 gtgacttcag gaatcatgag catgaaacga ccggtgatgc tgccgagcac tttcaggtag
      661 atgccgagca cgcgggcttc tgggccgcca aacaggccgg gaatctggtt gatcggcaga
      721 atcttcgctt gcggcacatc catgtcgatt ttgcggttga gaaactgcgc caacgcggtg
      781 gcggcgttcc ctgagccgat gttgccgatt tcccgcagcg catcgagctg gtcacggcgg
      841 atgacgtcaa agtcactcat ggcaagccag tccttctttt acaagtaaag gcattatacc
      901 gaacgcctgt gcgaattgca atgctcgaaa cgcgtgagaa acgcggccgg tcaatcgagt
      961 ggcgcgcggg gatgaggcgg cgatggaacc agtgcgggtt ctggttcggg cagataggat
     1021 ttgccgttga agaccgacat ggcgcgtgag cccatgcctg ccatcaacag acacaccatc
     1081 tgaggaatat ctttcacaac gtcgagcagg acatcgtgtt cctgctgcga aatggggccg
     1141 agaacgtgcg caaccaggtt gccatccggg ctgccgacgc cgatacgaat gcgccagaat
     1201 ccttctccga tgctgccgat gatggatttc agtccgttgt gcccgccggc actgccgttc
     1261 ggcttgatgc gcactttacc cacgggcagg cagacatcat cgatgatgac gatcagttcg
     1321 gccggagtgg ctcgctcctc ctgcaacagt ggccccaggg attcaccgga cagattcatg
     1381 tacgtctgcg gcttcaccag caggtgacga ctgcccttga acgagaactc gccgacgcgg
     1441 gcgcgacacc ggtgaccgga aagctgggtg cggaaatgac gcgtcaaggc atcgaccgca
     1501 atgaacccca gattgtggcg ggtgcggtca tattctggcc caggatttcc gagaccggcg
     1561 atgatcatgg ttggatgggt tattgtatgg tgcgggcgag aggctgtcaa acctcaccgg
     1621 ggaaaaacct tgacaggcct ttgtctccaa tggtaccctg acggggcaaa atacggatgt
     1681 attcagcgtt ttaacccact caggagttgc tctgatgaag cagcggattg gattttttgt
     1741 gctggtgagc ctgttcgtga tcgcgtgcac tgcgtttgcc gcgctcgaac aacagatgaa
     1801 cactctgtct cctcttcgcg agggcgatgt tctgcgtatc aaggtcatga acaatcgcag
     1861 tctggttgag atcgaccagg atgtgacggt gaacgcgaat ctcgatatcg atgtg
//
LOCUS       DAORHD010001050         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77477_length_1912_cov_2.256866, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001050 DAORHD010000000
VERSION     DAORHD010001050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77477_length_1912_cov_2.256866"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKO06_18515"
     CDS             complement(<1..838)
                     /locus_tag="PKO06_18515"
                     /inference="COORDINATES: protein motif:HMM:NF013358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HNV71705.1"
                     /translation="MIKRIDIAPPHNEVFGNPAPLGLIGLAVACAAITPVAFKFTLTA
                     EALKTAAIYCVLFGGACQFITGIMEFANKNVYGGTIFTAFAFNWIITGWTFYALAQGF
                     MPDHHVLLAVEITLFVIFVILTYGFGFFSKLLFFFLVDIDLLYVCKIAKALTHHPMLD
                     FPIALLTVILGFMGFWLAMAALINPLARQEIFKTTGPMFVPQRRKPFDWSLRRAIFDI
                     LYHQWRQRAFEEMTIDEFRKQLKAKVGERNPLPDIWYLKEYGCLELTVDPSDKNKILS
                     MRL"
     gene            complement(1106..>1912)
                     /locus_tag="PKO06_18520"
     CDS             complement(1106..>1912)
                     /locus_tag="PKO06_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71706.1"
                     /translation="IRTLMKRESDPNFKVNIVAHSKGNVVTRIYCSDAPQIFPKKTFL
                     SPFRNDVDTYVGIGPAFRGIDTPFRYYGYNLQIVTQKNSNGPVACDSMLLYGMWKSLK
                     DMSLYKNSSNFFPGQCQLLYNLVRDANVPLGPESMTPDMVTPYTLYFGGISALMTSRG
                     IDKAIDEGERLIYRLEEQGFNPNVKIGVIAGSSPFITYKDAHTLTPMPWEFFTPAGDG
                     LVYLASALFTDNAVKRGAKLIGKTTVDLNHVDIGRKKEIVRIIDDWLQAN"
BASE COUNT          446 a          456 c          594 g          416 t
ORIGIN      
        1 tcagacgcat gctgagaatc ttgttcttgt cggagggatc aactgtgagc tcaaggcaac
       61 cgtattcctt gagataccag atatccggca gcgggttgcg ctcgccgacc ttggctttga
      121 gttgcttgcg gaactcatcg attgtcattt cctcgaaagc gcgttgtcgc cactgatgat
      181 agagaatgtc gaaaatggcg cggcgcaggg accagtcgaa cggtttccgg cgttgcggca
      241 cgaacatcgg gccggtggtc ttgaagattt cctgtcgtgc cagcgggttg atgagcgcgg
      301 ccatcgccag ccagaaaccc atgaaaccca gaatcaccgt caacagggca atcgggaagt
      361 cgagcatcgg atggtgcgtg agcgctttgg caattttgca gacatacagc agatcgatgt
      421 cgacgagaaa gaagaacagc agtttactga agaacccgaa gccgtaggtg agaatcacga
      481 aaatgacaaa aagggtgatt tcgacggcca gcaggacatg atgatcaggc atgaaacctt
      541 gtgccagagc atagaaagtc catccggtga tgatccagtt gaacgcgaag gcggtgaaga
      601 tggtgccgcc gtagacgttc ttgttggcga attccatgat gccggtgatg aactggcagg
      661 cgccaccgaa caacacgcaa tagatggccg ctgtcttcag ggcttcggcg gtcagggtga
      721 acttgaaagc cacgggggtg atcgcggcgc aggcgaccgc gaggccaatg agacccagcg
      781 gggccgggtt cccaaatact tcgttgtgtg gcggggcaat gtcaatgcgt ttgatcatga
      841 gggcgtcctc ccggattttg ttgggcagca tgatagcatg gatgagttcc tcttgttcaa
      901 cattgaatat gcaaagtctg aaatgatggt atggacaaca gaatgcgctg agtctgcttg
      961 tactgttgcg gcgctcgtgg gtgtgaaagt cgtcgtgatt cgacgtcgta tgcgtgacag
     1021 tctcgcgggg agagaccgaa ggaagctgca gaaccgagaa agaagaaccg cccggcgaat
     1081 gccgggcggt gtgcgtgcga tcagatcagt tggcctgcag ccaatcgtcg atgatgcgaa
     1141 cgatctcttt cttgcggccg atgtcgacgt ggttcaagtc gacggtggtt ttgccaatca
     1201 gcttggcacc acgttttacg gcgttgtcgg tgaacagggc cgaagccaga tagaccagcc
     1261 cgtctcccgc gggggtgaag aattcccaag gcatcggggt cagagtgtgc gcatctttgt
     1321 aggtgatgaa cgggcttgat ccggcaatga cgccgatctt cacgttggga ttgaacccct
     1381 gttcctcgag gcgataaatc agcctttcgc cctcgtcgat tgctttatcg atgccgcgtg
     1441 aggtcatgag cgcggagatg ccgccaaaat agagggtata cggggtaacc atgtcgggag
     1501 tcatactctc ggggccaagc gggacgttgg catcacgcac caggttgtaa agcaactggc
     1561 actgcccggg gaaaaaattc gaagagttct tatacagaga catatccttc aaacttttcc
     1621 acattccgta aagcagcatg ctgtcgcagg cgacgggccc gttggagttc ttctgcgtga
     1681 cgatctgcag gttgtacccg tagtagcgaa atggagtatc gataccgcga aaggccggcc
     1741 caatgcccac atacgtatcg acgtcattgc ggaacgggga gagaaacgtc tttttgggaa
     1801 agatctgcgg agcatcggag cagtagatgc gggtgacgac attgcctttc gagtgagcga
     1861 cgatgttcac cttgaaattc gggtcacttt cgcgcttcat cagggtgcgg at
//
LOCUS       DAORHD010001051         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77604_length_1910_cov_3.064151, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001051 DAORHD010000000
VERSION     DAORHD010001051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77604_length_1910_cov_3.064151"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1910)
                     /locus_tag="PKO06_18525"
     CDS             complement(<1..>1910)
                     /locus_tag="PKO06_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71707.1"
                     /translation="TDISASAAVASGADTLVISTLSGPEYSEEEPGLGVASISADVQA
                     VDNVTASGPNIASISMNAFFDYWRPMASFGLPLEGRKYCKNQLQLQVEAKDRGSDSEL
                     QITRCEYKTDVSSWTSVVCDPPLPADSASTTFWIDITSIPEGEFGLSVRSFDRAGNHS
                     DNDDPTTTEPKPTDVSITIDRTPPVAPTLILPLDGTASRLRGYNFKWSKVTDGQMYLF
                     QIADDSAFNNILNNTAQVLPATYGTIIGELTPMTQAAFSAPKDGVYYWRVAALELCQD
                     GYNISKWSTTWRLVVDTVKPRVLEVQPTPSTGNKVSSGLVTFTIRFSENMDVTVMPTV
                     TLTSAGGEQMKIEQQTYRDNTWTGTTIIPKNSSALYDGNAVISISGAKDLAGNEMQTD
                     STNQIIVNTGPAFETKIFSNPAHEWEIVVVTRSTEALQAPPTCAVTQSGNRVPVTMNF
                     LKEKYYAGAYRINPDQPGKAYIDVYGTDLFGMVGQGSVEFIVANLNSNIKAKLSTSDN
                     LATLDIDAGTVAKKTNLYILPRSVVEGSTTGSANGSKAMSGALKANRAYSSAGTRPTS
                     GDLLELETLEEVGPRSVSLLKPMKFTASIKNEFGGHDLKKVGLYRHDGSRWIYCGPVS
                     GGNKVSASITGTG"
BASE COUNT          369 a          560 c          547 g          434 t
ORIGIN      
        1 ccggttccgg tgatcgacgc cgagaccttg ttcccgcccg agaccgggcc gcagtagatc
       61 cagcgtgaac catcgtgtcg atagagcccg accttcttca ggtcatgacc accaaactcg
      121 ttcttgatcg acgccgtaaa cttcatcggc ttcaagagac tgaccgagcg cggtccgact
      181 tcttcgagtg tctccagttc gaggagatcg ccgctggtcg ggcgggtccc tgccgagctg
      241 taggctcgat tcgctttcag cgctccgctc atggccttcg acccgttcgc ggagcctgtc
      301 gtgctgcctt cgaccactga acgcggcaga atgtacaagt tcgtcttctt cgccaccgtt
      361 ccggcatcaa tgtcgagagt cgccagattg tcagaggtcg acagcttggc cttgatgttc
      421 gaattgaggt tggcaacgat gaactcgacg gaaccctgac cgaccatgcc gaacagatcg
      481 gtgccataca catcgatgta ggcttttccc ggctgatcgg gattgatgcg ataggccccg
      541 gcgtaatact tttccttcag gaagttcatg gtcaccggca cgcggttgcc gctctgggtc
      601 accgcacagg tcggcggagc ctgcagggct tcggttgatc gagtgaccac gacaatctcc
      661 cattcatgcg ccggattcga gaagatcttg gtctcgaagg ccggaccggt attcacgata
      721 atctgattcg tggaatcagt ctgcatctcg tttccggcca ggtctttggc tccagagatc
      781 gagatcaccg cattcccatc atagagagcg ctgctgttct tcggaataat cgtcgttccg
      841 gtccaggtgt tgtcccgata ggtctgctgt tcaatcttca tctgctcgcc accggctgag
      901 gtcaaagtca cggtgggcat gacggtcaca tccatgttct cactgaaccg aatcgtgaaa
      961 gtcacgagac cagacgacac cttgtttccg gtcgaaggag tgggctgcac ttcgagcacg
     1021 cgaggtttca cggtatcgac caccagtcgc caggtcgtcg accatttgct gatgttgtac
     1081 ccatcctggc agagctcgag ggccgccaca cgccagtagt agacaccatc cttcggagcg
     1141 gagaacgccg cctgcgtcat cggagtgagt tcgccgatga tcgttccgta cgtcgccggc
     1201 agcacctgcg cggtgttgtt gaggatgtta ttgaacgcgg aatcgtcagc gatctggaac
     1261 agatacatct gtccgtccgt cactttgctc cacttgaagt tgtagccacg gagacgactc
     1321 gcggtgccat ccagcggcag aatcaacgtc ggagccacgg gaggcgtgcg atcgatcgta
     1381 attgagacat ctgtcggttt cggttcggtg gtggtcgggt catcgttgtc cgagtggttg
     1441 cccgcgcggt cgaagctgcg caccgataaa ccgaattccc cttcaggaat actggtgatg
     1501 tcgatccaga aggtcgtcga cgcagaatcc gctggcagcg gcggatcgca cacaacggaa
     1561 gtccagctcg acacatccgt tttgtattca cagcgagtga tctgcagttc gctgtcactg
     1621 ccgcgatcct tcgcttccac ctgcaactgc agctgattct tgcagtattt ccttccctcg
     1681 agcggcaggc cgaacgaggc catcgggcgc cagtaatcga agaacgcgtt catggagata
     1741 ctggcaatgt ttggcccgct cgcggtcacg ttgtcgactg cctgaacgtc agccgagatc
     1801 gaggcaacac ccagtccagg ctcttcttca ctgtattcgg ggcccgacag cgtgctgatg
     1861 accagggtat ctgctccgct ggcgactgcg gccgaggcac tgatgtcagt
//
LOCUS       DAORHD010001052         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77736_length_1908_cov_3.170534, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001052 DAORHD010000000
VERSION     DAORHD010001052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77736_length_1908_cov_3.170534"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKO06_18530"
     CDS             complement(<1..906)
                     /locus_tag="PKO06_18530"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71708.1"
                     /translation="MVKRLLLSACCLLLLYAPVASAQTRYQKAMAAFASGKLEVGVKE
                     YYASILLEDPYLSPSCKQRDLEGAEKHFQLKLDQASSRDWPAFYLALVKRLKGDLQTG
                     HGLVDEVRGRHPKSRILTFVKGEFLLAAEQYEEANRAFAHLATDRKSVSLVKLARHLQ
                     NRYGQATASSESRRRLLEQSYRFFDLGDYERAEKAMREFQTRFPDDMEGTGFLLEILL
                     AQDRATEAGTLHDLWKKQYGRSPLPPMREANLAYLLARFDRANELLIPMLAAQPDNDH
                     ARSLLAESLFQQGGYASASVHFAQLL"
     gene            complement(926..>1908)
                     /locus_tag="PKO06_18535"
     CDS             complement(926..>1908)
                     /locus_tag="PKO06_18535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV71709.1"
                     /translation="IVPNGIILFQARSRMYFKKRGEKFEIVTPTAVIGIRGTLFDLTV
                     EGRKTQITLLDGQVTAAAKKAPGDVTTLTAGNVCEVTESGCQVRPIAAPDVLDWYTEI
                     QMIPESSDQHRFDRLFLTGMTTLRQGRATLADNGQEGALTTGQKIPIGGTVRTNDEER
                     AEIRFACGSSLRLAPATELQVRDFGVALIRGAILVRHTRQNFPLKVIGPGTILVDSDS
                     VGDVQASGDSLFVRVQKGTIRLTPGNQSFSAGETVRVSANEVQHQGVIMPAHNWPLQP
                     QTSDAIVADELATAAGSLFEDAVVTDETQTTTTSDQDQSHDSDLKDILKP"
BASE COUNT          385 a          545 c          548 g          430 t
ORIGIN      
        1 caggagctga gcgaaatgga cggaagcgga ggcatagccc ccctgctgaa agaggctttc
       61 cgccagcagc gagcgagcgt ggtcattgtc cggctgtgcg gcaagcatcg gtatcagcag
      121 ttcgttggcg cgatcgaacc gtgccagcag ataggccaga ttggcttcgc gcatcggtgg
      181 cagcggcgat cgaccgtact gcttcttcca gagatcgtgc agggttccgg cttccgtagc
      241 ccgatcctgg gccaggagga tttcgagcag aaaaccggtg ccttccatgt catccggaaa
      301 ccgggtctga aactcgcgca tcgctttctc tgcgcgttcg taatcgccga gatcaaagaa
      361 tcgatacgac tgttccagca gacgcctgcg cgactcgctt gatgccgtcg cttgtccata
      421 gcggttttga aggtgtctgg ccagtttcac caaagaaacc gacttgcgat cggtcgccag
      481 atgagcaaac gcacggtttg cctcttcata ctgttcagcc gcgagcagaa actctccttt
      541 gacgaaggtg agaatgcgcg acttcgggtg acgaccacgg acctcatcga ccagaccatg
      601 accggtctgc agatctccct tcagtcgttt caccagggca agataaaacg ccggccagtc
      661 gcgggatgag gcctgatcaa gtttgagctg aaagtgtttc tccgcccctt caagatctcg
      721 ttgtttgcat gaagggctga gatacgggtc ttccagaaga atcgaagcgt agtattcttt
      781 gactcccact tccagcttcc cgctcgcaaa ggccgccatc gctttctgat atcgggtctg
      841 ggctgacgcg acaggggcgt agagcaggag cagacagcag gctgacaaga gaaggcgttt
      901 caccatcagt tccttgcgcg aattgtcagg gtttcagaat gtctttcagg tccgaatcgt
      961 gcgactgatc ctgatctgat gtggttgtcg tctgagtctc gtcagtgaca accgcatctt
     1021 caaacagcga accggcagct gtggccagct catcggccac aatcgcatcc gaggtctgtg
     1081 gctgcagcgg ccagttgtgg gcaggcatga tcacgccctg atgctgcact tcgtttgcgc
     1141 ttacccggac agtttctcct gcagagaacg attggttacc cggtgtcagt cgaattgttc
     1201 ccttctgcac gcgaacgaac agggaatctc cagatgcttg aacatcgccg acggaatcgc
     1261 tgtctaccag gatcgttccc ggtccgatga ctttgagcgg aaagttctgg cgggtatgac
     1321 gtaccaggat agcaccgcgg atcagggcga ctccgaagtc cctgacctgc aactcggtcg
     1381 caggtgccaa tctcagactt gagccgcaag cgaagcgaat ctcggctcgt tcctcgtcat
     1441 tcgttctcac ggttcccccg atcggaatct tctgtccagt cgtgagtgcg ccttcctgcc
     1501 cgttgtcagc cagcgtggcg cgaccttgtc gcagcgtggt catgccggtg aggaacaggc
     1561 gatcaaaacg gtgctggtcg ctggattcgg ggatcatctg tatttcagtg taccagtcga
     1621 gaacatcggg ggccgcgatc ggacgcactt gacagcctga ttccgtgact tcgcagacat
     1681 ttcctgcagt caaggtggtg acatctcctg gcgctttctt cgcggccgcc gtcacctgcc
     1741 cgtcgagcag cgtgatctga gttttccgac cctcgaccgt cagatcgaac agagtcccgc
     1801 gaatgccaat gacggcggtt ggcgtgacaa tctcgaactt ctctccccgt ttcttgaagt
     1861 acatccgtga cctggcctga aagaggatga tgccgttcgg gacgatct
//
LOCUS       DAORHD010001053         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77746_length_1908_cov_2.877496, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001053 DAORHD010000000
VERSION     DAORHD010001053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77746_length_1908_cov_2.877496"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..587)
                     /locus_tag="PKO06_18540"
     CDS             complement(147..587)
                     /locus_tag="PKO06_18540"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV71710.1"
                     /translation="MSSEACPMTPKAEFERQLQMLRQVKRIAVIGMSPNTDRPSHEVG
                     VYLHEQGYEVVPVHPAAKTIAGLTVQPTLTAAATTGAIDLVDLFVAGERTLPLVREAH
                     SLGIKRVWFQPGAENDEAEKVARELSLEVVSHACTMAVLKRANG"
     gene            complement(732..1304)
                     /locus_tag="PKO06_18545"
     CDS             complement(732..1304)
                     /locus_tag="PKO06_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV71711.1"
                     /translation="MPGQPDYTACMVREAPLHHYRICPRCKKDVSSSQRFCPSCYLWL
                     ARARQEPLRPLQLETDGWRDWVDRVWFPTRVFQFFLHHVILGIVVGIILATTLVVYIL
                     TLLPNWNIFGPRTHQRACYANMRVIQTSIEQYVMEQRFSPALASDPVKVLYEVGILSN
                     KPQCPIAGNVYKIPRGSSLQCVGSAGHGLP"
     gene            1454..>1908
                     /locus_tag="PKO06_18550"
     CDS             1454..>1908
                     /locus_tag="PKO06_18550"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV71712.1"
                     /translation="MKVVISGGSDIGYLCARELAPHHDLFVIEMNAALLERLEQLDVQ
                     IVSGNPTSLEVLREAQVSEADFFIAAAHSDEVNIISSLAVKQLSKAKTFCFVNKAHYF
                     ETFLGELGEHLIIDHLVWPEKLLAEDISRIITVPGATDIKVFEKDDLKLL"
BASE COUNT          406 a          570 c          537 g          395 t
ORIGIN      
        1 gttgcaacgc atactcgagg agcggcccgc attcaccgca gtagccccac gccgtgccat
       61 ggcgcacacg acagtacaac ttgaccatgg cttcgatggt ctgttgttcg cggtgcaggc
      121 gtgacataac ggggagagtc ggaggctcag ccgttggcgc gtttcaatac ggccatggtg
      181 caggcatggc tgaccacctc gagactaagt tcacgggcaa ccttctcagc ttcgtcgttc
      241 tcggcccccg gctggaacca gactcgcttg atgccgagtg aatgcgcttc tcgcacgagc
      301 ggcagcgtgc gctcgcccgc gacgaacaga tcgaccagat cgatcgcgcc ggtcgtggcc
      361 gcggctgtca gcgttggttg aacggtcaac ccggcaatgg ttttggccgc ggggtgaacc
      421 ggaacaacct cgtagccctg ctcgtgcaga taaacgccaa cctcatgact cggacgatcg
      481 gtgttgggcg acatgccgat caccgcaatg cgtttcacct ggcgcagcat ctgcagctgg
      541 cgttcgaact cggctttcgg ggtcatcggg caggcctcag aactcatgtg tcgtcgcctc
      601 cgctgtggaa tgaccgcatt ctacgccatg caaccggcac tggcaagacg tgttctcgct
      661 gggccagggt catttcgttc ttcagaccgc gttttccccc tggcatcgct caacgattca
      721 ccagcgcagc gttagggtaa cccgtgtccc gcggaaccca cgcactgcag acttgaaccg
      781 cgagggatct tgtagacatt acccgcaatc gggcattgcg gcttgttcga caggattccc
      841 acctcgtaca aaaccttcac cggatcgctg gccaaggctg gtgaaaaccg ttgttccatc
      901 acatactgtt cgatactggt ttgaatgacg cgcatgttgg catagcaggc acgctggtgc
      961 gtgcgcgggc caaagatatt ccagttcggt agaagcgtca gaatatagac gacgagcgtc
     1021 gtcgcgagaa tgatgccgac gacgatgcca agaatgacgt ggtgcagaaa gaactggaag
     1081 acacgggtgg gaaaccagac ccgatcgacc cagtcacgcc agccatccgt ctcgagctgc
     1141 agcgggcgca gcggctcctg cctggcgcga gccagccaca gataacaact cgggcagaat
     1201 ctctgactgc tgctgacatc tttcttgcag cgcggacaga tgcggtaatg atgcaggggt
     1261 gcttcgcgaa ccatacaggc agtatagtcc ggttggcccg gcattgccac ctcatacgca
     1321 aacccttttc tccatcgtcg ttgacgtccg actcgccatc caggcagtcc tcatgtataa
     1381 tcccagccca accggactca cgttctcgtg tcggtcatcc ggtgaaatgc tttgatcaca
     1441 ggcggtgaca tccgtgaaag tcgtcatctc gggcggcagc gacatcgggt atctgtgcgc
     1501 acgcgaactg gctccgcatc acgatctgtt cgtcatcgag atgaacgccg cgctactgga
     1561 acggctggaa cagctcgatg tccagatcgt ctcagggaat ccgacgagtc tcgaagtgct
     1621 ccgggaagcc caggtgtcag aagcggactt cttcattgcc gccgcccatt ctgatgaagt
     1681 caacatcatc tcttcgctgg cggtgaaaca gctcagcaag gcgaaaacct tctgcttcgt
     1741 caacaaggcc cactatttcg aaacctttct cggcgagttg ggagagcacc tgatcattga
     1801 tcatctcgtc tggcccgaga aacttctggc cgaagatatc tctcgcatca tcacggtacc
     1861 gggcgcaacc gatatcaaag tctttgaaaa agatgatctg aagctgct
//
LOCUS       DAORHD010001054         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_77863_length_1906_cov_3.092923, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001054 DAORHD010000000
VERSION     DAORHD010001054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77863_length_1906_cov_3.092923"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1906
                     /locus_tag="PKO06_18555"
     CDS             <1..>1906
                     /locus_tag="PKO06_18555"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV71713.1"
                     /translation="TVQDVLDLINNHNGGMTVQAKFDMVHHRLELTDLTGGKGDFSVE
                     SKQNQLFLKDLPAHAAADLGLLKDVGAASTILSTFDSALESEATPLSFLNGGLGVERG
                     FIRITGHDGVATDVNLSAATTVQEVIDAINAQTGGNQTASYDTTTGTFRIDDNTVGTN
                     AFHIDEVNGAAPVTVREPATIAKRLGLLKSSQGNNLLGDSLQPGGLTTASALSSIVPP
                     PEKGIMVLRGADGNPVEVDLARAGTIQDVLSAIDATQKFSATWDVPAQRFIITDVSGE
                     PGSEGISAEERTNTARDLGFLTGAINYQQDTLTGRPISVKNLPTLVGSIDLNPAVSGS
                     TTIDSLNAGRTFNQGAQLGRIRITDKAGHFLAIDLRGSKTIQDVLNKINDPANGIYVE
                     AKINADRNGIEIIDKNRGATGKLEIIDVDSTAAYDLGISTPPGGTVDKRWVGRDIDPA
                     VTDETSISDLRVNEGGIPLGKVYVQSGEYSGEIDLTGARTVKELLDKLSLSDMNANLT
                     AWISEDGKRLNLSNTKGQAYIKVRDIGTEPEQRTASGLGLGGSRGIFETLMDLRDNLL
                     RNDSKAISEVSIKDIQTDITRVLKYHTEVGAKTNRATGAKEKLENFNLNLKKVLSDVE
                     DVDMTEAITRM"
BASE COUNT          460 a          508 c          555 g          383 t
ORIGIN      
        1 aaccgtgcag gatgtgctcg atctgatcaa caaccataac ggtggcatga cggtgcaggc
       61 taaattcgac atggtgcatc atcgccttga actcaccgat ctgacggggg gaaaagggga
      121 cttctcggtc gaatcgaaac agaaccagct gtttctgaaa gatctgccgg cgcatgcggc
      181 tgctgatctc gggttgctga aagatgtcgg ggccgccagt acgattctct cgacttttga
      241 ttcggctctg gagtcggaag ccacgcctct gtcatttctg aacggtggcc tgggcgttga
      301 acgtggcttc attcgcatta ccggccacga tggagtggcg acggatgtca atctgagtgc
      361 cgctacgacg gtgcaggaag tcattgatgc catcaatgcc cagacggggg gcaatcagac
      421 cgcgagttac gacacaacaa ccggtacatt tcgcatcgat gacaatacgg tcggcacgaa
      481 cgcgtttcac atcgacgaag tgaatggagc tgccccggta accgtgcgcg aaccggcaac
      541 cattgccaaa cgactcggac tgttgaagtc gtctcagggc aacaacctgc tgggtgattc
      601 gttgcagccc ggtgggctca cgaccgcttc cgctctgtct tccatcgtgc caccgcccga
      661 aaagggaatc atggttctgc gcggagctga cggcaacccg gtcgaagtgg acctggcacg
      721 agcgggtacc attcaggatg tcctgagtgc catcgatgcg acgcagaagt tctccgcgac
      781 gtgggatgtt ccagcacagc ggttcatcat caccgacgtt tcgggtgagc caggctcaga
      841 gggtatttcg gccgaagagc gaacgaacac cgctcgcgac ctcggttttc tgaccggggc
      901 gatcaattac cagcaggata cgttgaccgg tcgtcccatt tcggtcaaga atctcccgac
      961 gctggtcggc tccattgatt tgaaccctgc tgtgagtggc tcaactacta ttgatagcct
     1021 aaatgcgggt aggactttca atcagggtgc gcaactcggg cgcattcgca ttactgacaa
     1081 ggccggccac tttctggcca tcgatctgcg cggttccaaa acgattcagg atgtgctcaa
     1141 caagatcaac gacccggcga atggcatcta cgttgaggca aaaatcaatg ccgatcgcaa
     1201 tggcattgag atcatcgaca agaatcgtgg cgcgaccgga aaactcgaga tcatcgatgt
     1261 cgactcgacc gcggcatatg atttgggcat cagcacgccc cctggtggga ccgtcgacaa
     1321 gcgctgggtg ggtcgcgata ttgacccggc ggtcacggat gaaaccagca tcagcgacct
     1381 gcgtgtgaac gagggtggca ttccactggg taaggtctat gtgcagagtg gcgaatactc
     1441 gggcgaaatc gacctgaccg gcgcacgcac ggtcaaggaa ctgctcgata agctgtcgct
     1501 gagtgatatg aacgcgaacc tgacggcgtg gatttccgag gacggaaagc ggttgaacct
     1561 ctcgaacacc aagggacagg cctacatcaa ggtgcgcgat atcggcacgg aaccggaaca
     1621 gcgaaccgcg tcagggcttg gtctaggggg gtctcgcggt atcttcgaaa ccctcatgga
     1681 tctgcgtgac aacctgctgc gcaatgacag caaagccatc tcagaagtca gcatcaagga
     1741 cattcagacg gatatcaccc gtgttctgaa gtatcacacg gaagtcggtg ccaagaccaa
     1801 tcgggcgact ggtgccaaag aaaaactcga aaatttcaat ttgaacctaa agaaagtgct
     1861 cagtgatgtc gaagatgtag acatgacaga agccattact cgcatg
//
LOCUS       DAORHD010001055         1902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78105_length_1902_cov_2.972388, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001055 DAORHD010000000
VERSION     DAORHD010001055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78105_length_1902_cov_2.972388"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..826)
                     /locus_tag="PKO06_18560"
     CDS             complement(161..826)
                     /locus_tag="PKO06_18560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71714.1"
                     /translation="MKRISSIVLFLFLVTFSFSAGALDFPVTCTIDLEKDSIDNKNPL
                     AIQVHDRFSDHMAVVRITLDPQKTEMRAARFEIEYGEETTGWTVNIGDSVSNNGHGGD
                     SANQTHDSEVQILDGDLTLIGSDLAPQPPEKVVKSIKRFAQPKSTVKLEVSNEKLAWV
                     TADGSQGEIRSPFIYALNGQRDSEGQSNFDIYAAFNRVVSDTSRNGRGVKRVYITLLP
                     DKI"
     gene            complement(889..1572)
                     /locus_tag="PKO06_18565"
     CDS             complement(889..1572)
                     /locus_tag="PKO06_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71715.1"
                     /translation="MRRGDIIWGTLLVGISGLLVFPATHALFVDTTRAHPYLMGFLKV
                     SILASMGELLARRITRHNWDFPVGMPWRVVVWGGIGMSFVAVFELFASGTTALINKSL
                     LPTLTGETGNRLAQAFWTSTLMNILFAPGFMAFHRLTDGFIEEGHGTWAGIRSATLHA
                     VMQKQDWSGFVGFVLLRTIPLFWIPAHTITFLLAPEYRVLMASYLSIALGGILAFARL
                     RTAPAQTSA"
     gene            1721..>1902
                     /locus_tag="PKO06_18570"
     CDS             1721..>1902
                     /locus_tag="PKO06_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71716.1"
                     /translation="MRSISRGMMSLRRRMLGILVAGLATLWLSGCTSESGNKIAVQTV
                     GTGSPQQEWGTEYPIDA"
BASE COUNT          447 a          504 c          546 g          405 t
ORIGIN      
        1 tgcttgagct ttctgtgcga ctttaggggc gacgacttca tacaatttga ttgccggcgc
       61 cagtggtttg acctgcaaca gcttatacac gaccgacctc caatcattcg attctgagat
      121 ttttgcgcta tcttaccggg ttgaggcgtt cgatgcaaga tcagatcttg tcgggcagca
      181 gggtgatgta gacccgtttc accccccggc cgtttctcga ggtatcgctg acaacacggt
      241 tgaaagcggc gtagatgtcg aaattggact ggccttccga gtcacgctgt ccgttcaggg
      301 catagataaa tggtgagcga atctctccct gacttccgtc ggcagtgacc caggccaact
      361 tctcgttgct gacttccagt ttgaccgtcg acttgggttg agcgaatctc ttgatgcttt
      421 tcacgacttt ttcaggtggt tgaggagcaa gatcactgcc gatcagggtg aggtcgccgt
      481 cgaggatctg aacttcgctg tcgtgcgtct gattcgcact gtcgccaccg tgaccattgt
      541 tgctgactga atccccgatg ttcaccgtcc agccggtggt ttcctctcca tactcgatct
      601 cgaaacgagc cgcccgcatt tcggttttct gtgggtcaag cgtgattcgg accacggcca
      661 tgtgatccga aaaacgatcg tgaacctgta tggccagcgg attcttgttg tcgatgctgt
      721 ctttttcgag atcaatagta caggtgactg gaaagtccaa tgcgcctgca gagaacgaaa
      781 aagtgaccag gaaaagaaac aggacaatcg aactgatacg tttcataacg ccctcctgaa
      841 aattggatca gggtcagagg aacagaaccg ggcaaagatc gaaacaaatc acgccgaagt
      901 ctgagccggc gcggtccgca agcgagcaaa cgccagaatc ccgccgagcg cgatcgagag
      961 ataggatgcc atgaggacgc gatattccgg agccagcaga aaggtgatgg tatgcgcggg
     1021 aatccagaac agcggaatcg tacgcaggag gacgaagccg acaaagccgc tccaatcctg
     1081 cttttgcatg accgcatgca gggtggctga gcggatcccc gcccaggtac catgcccctc
     1141 ttcgatgaat ccgtctgtca gacgatggaa tgccatgaat ccgggagcga agaggatatt
     1201 cattaaggta ctggtccaga aagcctgggc caggcggttt ccggtttcgc cggtcagtgt
     1261 cggtaacagg gacttgttga tcagggccgt cgtaccagac gcgaacagtt cgaagaccgc
     1321 cacgaacgac atgccgatgc ccccccacac cacaacgcgc catggcattc caacggggaa
     1381 gtcccagttg tgacgggtga tgcggcgcgc cagaagctca cccatcgatg ccagaatcga
     1441 caccttgaga aaacccatca gatagggatg cgcgcgcgtc gtgtcgacga acagtgcgtg
     1501 agtggccggg aagaccagaa gaccggaaat gccgaccagt aaagtgcccc aaatgatatc
     1561 gccgcgccgc atgcgtgact ccttgtgtcg tgttaaagcg gtgatgggct catcctaacg
     1621 caatcagtcg gaaaaatgca aaaatgccct tcggtttcga gcgcgtcatg ccgatgtacc
     1681 gataaaggga tgattttgtc cgcttgaagg gagtcgggtc atgcgatcga tctctcgagg
     1741 catgatgagt ctgagaagac gaatgctggg cattctggtc gcaggcctcg ccaccctctg
     1801 gctttccggg tgcacgtcag aatccgggaa caaaattgcg gttcagacgg ttggcacagg
     1861 cagcccgcaa caggagtggg gaaccgagta tcccatcgat gc
//
LOCUS       DAORHD010001056         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78150_length_1901_cov_4.496208, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001056 DAORHD010000000
VERSION     DAORHD010001056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78150_length_1901_cov_4.496208"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PKO06_18575"
     CDS             complement(<1..858)
                     /locus_tag="PKO06_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71717.1"
                     /translation="MNTLILSSTGAATGVKPLKGLGDALKAMTEGLYRSAYFFDSSES
                     PESVQQAISEEAIALSGPMVAVHAIRRASDLLLEECGYDGMDLYLEVEGKRVPELPGR
                     DDLLELFTKDYTHIVVDGKPYRPNQEVFGFQAVYDDTTRFVEQLKSLGIGSDALYMLA
                     TPDEIAVEIHPGVFGGGSHRQLGRMYRALMGRIAGIKRGERRLMKTTDRTIVPELALA
                     TAAILVPGSIHPGLHRPKVGVSQSHFAYGPAAFSDYCGKKRTLDECLKEVRTWIKFLE
                     STKEPIQKLK"
     gene            complement(869..>1901)
                     /locus_tag="PKO06_18580"
     CDS             complement(869..>1901)
                     /locus_tag="PKO06_18580"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNV71718.1"
                     /translation="GNQLITIMDLARNKGMKTGIISDTRLTHATPAAFAGHVAHRDDE
                     EKLAEQIVFAGIDVLMSGGRAWFQSVDQGGKQAKGRSLLADAEQQGYRMVQSRQDLVQ
                     ALTDKAEKVLALFADSYMPFSFEPDFKTTPDLPEMTASALRLLSTHPKGFLLVVEAGQ
                     IDALAHAHDAAELVEQIREMDATVRVLQEFVRSRPDTLLVMVPDHSTGGIHMAERFQE
                     DAFRKSAVSTKMLARRIGDAPETLAAELKRAFPALSFSEAELGALKPAPNDEEFEMKL
                     GDLVWGKLGIYFLDTVQSLKQMSTNGHGCEDMFLHAMGAHQNLFQGVMNLTDIPRRIA
                     AALQLGELR"
BASE COUNT          411 a          591 c          501 g          398 t
ORIGIN      
        1 tttcaacttc tgaatcggct ctttcgtcga ttcgagaaac ttgatccagg ttcgcacctc
       61 tttcagacat tcgtccaggg tgcgtttttt tccgcagtaa tccgaaaacg ccgcgggacc
      121 ataagcgaag tgcgactgac tcacaccgac tttggggcga tgcaaccccg gatgaatgct
      181 tccgggaacg agaatcgccg cggtcgcgag cgcgagctca ggtacaatcg tacgatccgt
      241 ggttttcatc aggcgtcgtt ccccgcgctt gatccccgcg atgcgcccca tcaacgcgcg
      301 atacatgcgc ccgagctggc gatgactgcc accgccgaac acgcccgggt gaatctcgac
      361 agcaatctcg tctggtgtgg ccagcatata caaagcgtca ctgccaatgc ccaacgattt
      421 cagctgttca acaaatcggg tggtgtcatc gtagaccgct tgaaagccaa agacttcctg
      481 attggggcgg taaggctttc cgtcgacgac gatatgcgtg taatctttgg taaacagctc
      541 caacaggtcg tcacgacccg ggagttccgg tactcttttt ccctcgacct cgagatacag
      601 atccatgccg tcgtaaccgc actcctcgag cagcagatct gaagcgcgac gaatggcatg
      661 aaccgccacc atcgggccag acaacgcgat tgcctcttca ctgatcgcct gctggacgga
      721 ctccgggctc tcagacgaat cgaagaaata ggccgaccgg tacaaacctt cggtcattgc
      781 cttcaaggca tcgcccagac ctttcagcgg tttgactccc gtggcagcac ccgtcgatga
      841 cagaatcagc gtattcatgt cccacctctc agcgtaattc tcccaattgc agcgcggctg
      901 caatgcggcg tggaatatca gtcaggttca tgaccccctg aaacaggttc tgatgagccc
      961 ccatcgcatg caggaacatg tcttcacagc cgtgaccgtt cgtcgacatc tgtttcagag
     1021 actgtacggt atcgagaaag tagatcccga gctttcccca gacgaggtca ccgagcttca
     1081 tctcgaactc ttcatcattc ggcgcgggtt tcagggcacc aagttcggcc tccgaaaacg
     1141 agagcgccgg aaaagcccgc ttcagttccg ccgccagagt ctctggagca tcaccgatac
     1201 ggcgcgccag cattttcgtc gagaccgccg atttgcggaa cgcatcttcc tggaaacgtt
     1261 cggccatgtg aatgccaccg gtcgaatggt cgggaaccat gacgagcagc gtatccgggc
     1321 gagagcgaac aaactcctgc aataccctca ccgtggcatc catctcacga atctgttcca
     1381 ccagctccgc ggcgtcatgc gcgtgcgcca gcgcatcgat ctgtcctgcc tcgaccacca
     1441 gcagaaaccc cttggggtgc gtggagagaa gacgcagggc cgaagccgtc atttcgggta
     1501 agtcgggcgt ggtcttgaaa tcaggctcaa aggagaacgg catgtaggaa tcggcaaaca
     1561 gggccagaac cttttccgcc ttgtcggtga gagcctgcac cagatcctgg cgtgactgca
     1621 ccatgcgata gccctgctgt tccgcgtccg caagcaggct acgtcccttg gcctgtttcc
     1681 cgccctgatc gacactctga aaccaggcgc gcccaccgct catcagcacg tcgattcccg
     1741 cgaatacgat ctgctcagcc agtttttcct cgtcgtcacg atgcgccacg tgcccggcaa
     1801 aagctgccgg agttgcgtgc gtcaagcggg tatcagagat aatgccggtc ttcatgccct
     1861 tgttccgcgc aagatccatg atggtgatca actgattgcc c
//
LOCUS       DAORHD010001057         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78151_length_1901_cov_4.468581, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001057 DAORHD010000000
VERSION     DAORHD010001057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78151_length_1901_cov_4.468581"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1901)
                     /locus_tag="PKO06_18585"
     CDS             complement(<1..>1901)
                     /locus_tag="PKO06_18585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71719.1"
                     /translation="DLVRSLLRQGEILLGRDDYATAIMLFREAERLSEEYRDQKQLHR
                     SWWLQALVHRRQGNFDEALKLLEQLRLVADEQSDPYFCEGILGETAGILYIQSQYTNA
                     QELHQKREVLCRRLGNMVGLAYALQGTAQVLRSRGDLKKAMQFLGEAESVCRRLGYQE
                     GLQSILTDQGIIAHARGELEEALRLFRSSELITRQQSIQVGLQRALGAQASVLRSRGD
                     LDGSFSLFREQELICRKLGDKEGLQIALGGQAVIRRERGQLNEAMRLARESEKLCREL
                     GYREGLLRVLSTQAMILRDRGDLDGAVGMLREGEAIGRELGLKSGLQRILESLGLVLK
                     ARGDMDGALALFREMERICREIGSLVGLQVALGHQAQILYFRGEVETAMSLFQEQERI
                     CRELGLKDRLQHSLGGQAVILRSRGHLDQALAMLKDQEGICRDLGLRAGLQISLYNQG
                     IIGLMRGDLNGALALFKQQEEICRDSGYKEGLQACIGSQAVILRNRGDLDGALRLFRE
                     QEAICREAGYKEGLQVSLCGQSAIHQARGEFERAAALLGEGERICRELNHREGLQRIL
                     SSKSVILQQKGDQAGAMETLKEAEKIARSLDHKSGLQRILEYQAGILKKNGDLASAMV
                     TTYERPDSV"
BASE COUNT          359 a          611 c          549 g          382 t
ORIGIN      
        1 gcacgctgtc gggccgctcg tacgtggtga ccatggcgct cgccaggtcg ccgttctttt
       61 tcagaatccc ggcttgatac tcgagaattc gttgcagacc cgacttgtga tcgagactgc
      121 gcgcgatctt ctcggcctct ttgagggttt ccatcgcacc ggcctgatca cccttctgtt
      181 gcagaatgac cgatttgctc gacagaatgc gttgcagccc ttctcggtgg ttcaactccc
      241 ggcagatgcg ctctccttca ccaagcaggg cggcggcgcg ttcgaactcg ccgcgggcct
      301 ggtgaatggc cgattgtccg cagagcgaca cctgtagccc ttccttgtag ccggcttccc
      361 gacagatcgc ttcctgttcg cggaacagac gcagcgcgcc gtcgagatcg ccgcggttgc
      421 gcagaatgac ggcctggctg ccgatacagg cctgcaggcc ttctttgtag ccagagtcgc
      481 ggcagatttc ttcctgttgt ttgaacagcg cgagagcgcc gttcaggtcg ccgcgcatca
      541 gcccgatgat gccctgattg tacagtgaga tctgcaggcc ggctctcagc ccaaggtcgc
      601 gacagatgcc ttcctgatct ttgagcatgg ccagagcctg atcgagatgc ccgcgtgagc
      661 gcagaatgac cgcttgcccc cccagcgagt gctgcagccg gtccttcaag cccagctctc
      721 gacagatccg ctcctgctcc tgaaacagag acatcgcggt ttcgacttcg ccacggaaat
      781 acagaatctg tgcctggtga cctagcgcga cctgcagacc gaccaggctg ccgatctcgc
      841 gacagatgcg ctccatttcg cggaacagag cgagcgcgcc atccatgtca ccgcgagctt
      901 taagtaccag tccgagactt tcgagaatgc gctgcagacc agacttgagg ccgagttctc
      961 tgccgatcgc ctcgccttcc cgcagcatgc cgaccgcgcc gtcgagatcg ccgcgatcgc
     1021 gcagaatcat ggcctgcgtc gataacacgc gcagcaagcc ttcacgatag ccgagttcgc
     1081 ggcagagctt ctcggactct cgcgccagcc gcatggcttc gttcagctgg ccccgctcac
     1141 gacgaatgac ggcctgaccg ccgagggcga tctgcaatcc ttccttgtca ccgagtttgc
     1201 ggcagatcaa ttcctgctca cgaaacagag aaaaagaccc atcgaggtcg ccgcgtgaac
     1261 gcaggaccga ggcctgtgcg ccaagggcgc gctgcagacc gacctgaatg ctctgttgtc
     1321 gggtgatgag ttcactggat ctgaacaggc gcagcgcttc ttcgagttcg ccgcgcgcat
     1381 gggcgatgat gccctgatcg gtgagaatgc tctgcaaacc ctcctgatat cccaggcggc
     1441 ggcagaccga ctcggcttcg ccgagaaact gcatggcctt cttcagatct ccgcgagagc
     1501 gaagaacctg ggctgttccc tgaagggcgt acgcgagccc caccatgttc ccgaggcgcc
     1561 ggcagagcac ttcacgcttc tgatgcaact cctgggcgtt ggtatactga ctctgaatgt
     1621 acaggatacc ggcggtttct cccagaattc cttcgcagaa atacgggtca gattgctcgt
     1681 cggcaaccag ccgcaactgc tcgagcagtt tcaaggcctc gtcgaaattg ccctgacggc
     1741 gatggacgag cgcttgcagc caccaggagc gatgcagctg cttctggtct cggtactctt
     1801 ccgagagacg ttctgcctcg cggaacagca tgatcgccgt cgcgtagtcg tcgcgaccga
     1861 gaagaatctc gccctggcgc agcagcgaac gcacgagatc g
//
LOCUS       DAORHD010001058         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78343_length_1899_cov_2.341106, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001058 DAORHD010000000
VERSION     DAORHD010001058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78343_length_1899_cov_2.341106"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="PKO06_18590"
     CDS             <1..1738
                     /locus_tag="PKO06_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71720.1"
                     /translation="LSALGIIIAGVLRYSGSELKLAQRLIREKRAEYLAYAGISWTLE
                     ELRKKRWYQPPGKPPLKVQRPNYHMEVLEPFGAGNGKVTVICDEYGKLGVDVSKSDLY
                     GNLDKADLLDHIKVFSIGEDGDAQTRAMVYGKFIMSPDPFLNSDSTDAPTMGSVAGKG
                     MMDILIPQAWSQDGAVGKTFIVHEILVTVGTRVDPNTVVARLRQGPDWPYPTTMTHDV
                     KSPYFGTLTTINVKTGQQVSQDDVFGTIKEESGSSVPSETLKRMVQVKRVDLRPFFGK
                     DLTDISTIREIGKVMGDESRLFVKNQVLNQPLSRSLAENLTKTPLPDKTDAATIMNRL
                     GGLGMGSSAGYDQDGNAFILDLFKHWHPRGYQLKDDEIPAFLAGCRFNLGTRPSEPRA
                     EIKQVLTYSGHKDVLDKLYDTAARKNPEYYVLKGIEKYLSMLKHDPNSVTEKMIHDLS
                     FLQNARKRISVKLDGGTPWEDDIYKDKVAKGENMAGYWWWPERAGWYGPIKISGPFPD
                     EVPYTLENDTVDPPLKIRVDYLLNYHRKHYDEGSLQPFPSGWRLPADQNDTPQRPGPP
                     DSTGSQYSGSAS"
BASE COUNT          453 a          532 c          523 g          391 t
ORIGIN      
        1 gctttccgcg cttggcatca tcattgccgg ggtccttcgc tattccggct cagaactgaa
       61 actggctcag cgactgatac gcgaaaaacg cgccgagtat ctggcctatg ccggcatcag
      121 ctggaccctc gaagagctac ggaaaaagcg atggtatcag cctcctggca agccaccgct
      181 caaggtacaa cgccccaact atcacatgga agtgctcgag cctttcggcg ccggaaacgg
      241 gaaagtcacg gtcatctgcg acgagtatgg caagcttggt gtcgacgtat cgaagtctga
      301 tttgtacggc aatctggaca aggccgatct gctcgatcac atcaaggtgt tcagcatcgg
      361 agaagatggt gacgcgcaaa ccagggccat ggtctacggc aagttcatca tgagtcctga
      421 tccgtttctc aactcagata gtactgatgc ccccaccatg ggatcagtcg ccggaaaagg
      481 gatgatggac attctgattc cgcaggcctg gtcgcaggat ggagcggttg gcaaaacctt
      541 cattgtccat gaaatcctcg tcacggtcgg cacacgagtt gatccgaaca ctgttgtcgc
      601 ccggctgcgg caggggcccg actggccata tccgacgacg atgacgcacg acgtgaagtc
      661 accctatttt ggtacgctca cgaccatcaa tgtgaagact ggtcagcagg tcagtcagga
      721 cgacgtcttc ggaaccatca aagaggagag cggctcttcg gtgccttcag aaactctgaa
      781 acgcatggtg caggtgaaga gagtcgatct gcggcccttc ttcggaaaag acctgaccga
      841 catctcgaca attcgtgaga tcggaaaagt catgggcgac gagagccgcc tgttcgtgaa
      901 gaatcaggtc ctgaatcaac cactcagtcg tagcctggca gagaatctca cgaaaacccc
      961 tctgcccgac aaaaccgatg ccgcaaccat catgaaccgt ctcggcgggc ttggaatggg
     1021 cagcagcgct gggtatgacc aggacggaaa cgccttcatt ctcgatctct tcaagcactg
     1081 gcatcctcgt ggctatcagt tgaaagacga cgagattcct gcgtttctgg ctggctgtcg
     1141 attcaatctc ggtacccgcc cgagtgagcc gcgcgctgag atcaagcagg ttttgaccta
     1201 ctcggggcac aaagatgtcc ttgacaagct ctatgacacg gctgcccgga aaaatccaga
     1261 gtactatgtg ctcaagggca tcgagaagta tctgagcatg ttgaagcatg atccgaacag
     1321 tgtgaccgaa aaaatgattc acgatctgag cttcctgcag aatgcccgaa aacgaatatc
     1381 ggtcaaactt gacggcggca ccccctggga agacgatatt tacaaggaca aagtcgccaa
     1441 gggagaaaac atggcgggtt attggtggtg gcctgaacga gccggctggt acggtcccat
     1501 caagatttcc ggcccgtttc ccgacgaagt gccttatacc ctcgaaaatg atacggtcga
     1561 cccaccgctg aaaatccggg ttgattatct gctcaactac catcgcaagc attatgacga
     1621 agggagtctg cagccgttcc ccagtggctg gcgactgccg gccgatcaga acgacacccc
     1681 ccagcgtccg ggtccccctg attcgacagg gtcgcagtac tcggggagtg ccagctaatg
     1741 cggtcttatc tgtgccgcgg aaaggaaacc atgatgcgga gtttgctcgt ccgacacccc
     1801 tttcgtctct ttgcctgtct ggtgttcagc ctgtcttttg ccgccgtgct tcaagccggg
     1861 gtactgctcg agggcgacaa aacaacgccc gccgctccg
//
LOCUS       DAORHD010001059         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78438_length_1897_cov_4.318132, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001059 DAORHD010000000
VERSION     DAORHD010001059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78438_length_1897_cov_4.318132"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1687
                     /locus_tag="PKO06_18595"
     CDS             <1..1687
                     /locus_tag="PKO06_18595"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNV71721.1"
                     /translation="KKDITLFLDAHVDVLMAFAKNYAQERINNVLSYISSQTEDITIR
                     LRSIEGEIKQLQESQKLISIPEEMATALSNEKKARSDLEQLEFETNNTKLAIDNLRGK
                     LGGTKVGVKSSISGDVVILLRKELTSLELESMSLSMVYKEDHPKILEVKDKIAFIRKK
                     IEEESLKGFSSVSDENLEVIKVSVGELVRLENQLIFLEARKAMIKQQIDKAEKGFIDL
                     SNNKQKYTDLVREQKVLEQSLASNIDRKNQALIEKQGVRSRLSVLARPWEPNFPVKPN
                     YKMYMLFTLVLAFLTGLGVVSLAEQIDNSVKSTQEIQEMLNLPIRGLIQDLYKCQKYV
                     PQTKDPVDPLLCTHLFPASKEAESFRTLRTNILHQHKENPFQTMMVVSATECVGKSTV
                     TSNLGIIIAQAGYRVIIVDTDLRHPTIHKKFRLENNKGLTNLLTGMPFDEVIQPTCIP
                     RLSILAAGGLPPNPAEILQNDGFQEVINFLKTQADFVLFDNPPLNLVTDSLILATKIQ
                     RVFYLVGIGHTDKNEIRTSFELLKGLNCNVAGLICNRIPPNEMGGYYYYYVKS"
BASE COUNT          481 a          492 c          501 g          423 t
ORIGIN      
        1 caagaaagac atcaccctgt ttctcgacgc gcatgtcgat gtgctgatgg cttttgccaa
       61 gaactatgcg caggaacgga tcaacaacgt tctttcgtat atcagttcgc agactgaaga
      121 catcacgatt cgtctgcgat cgatcgaagg cgagatcaaa cagctgcagg aatcacagaa
      181 actgatctcg atcccggaag aaatggcgac ggcgctgagc aacgagaaga aagcacgcag
      241 tgacctcgaa cagctggaat tcgaaaccaa caacaccaaa ctggccatcg acaatcttcg
      301 tggcaagctg ggtggcacga aagttggggt caagtcgagc atttccggag atgtggtcat
      361 actgctgcgc aaagagttga ccagtctcga actcgagtcg atgagcctgt cgatggtcta
      421 caaagaagat catccgaaaa ttctcgaggt gaaagacaag atcgcgttca ttcgcaagaa
      481 gatcgaagaa gagagtctga aagggtttag ttcggtcagc gatgaaaacc ttgaagtgat
      541 caaggtgagc gtgggtgagc tggtgcggct cgaaaaccag ctgatctttc tcgaagctcg
      601 caaggcgatg atcaagcaac agatcgataa ggccgaaaaa gggtttatcg acctgtcgaa
      661 caacaaacag aaatataccg atctcgtgcg tgaacagaag gttctcgagc agagtctcgc
      721 cagcaatatc gatcgcaaga atcaggcact gatcgagaaa cagggcgttc gcagccgact
      781 ttccgtgctg gcccgcccgt gggaacccaa ctttccggtc aagcccaatt ataaaatgta
      841 catgttgttc actctggttc tggccttcct gaccggtttg ggtgtcgtgt cattggcaga
      901 acagattgat aactcggtga agagcacgca ggagattcag gaaatgctga atctgccgat
      961 tcgtggcttg attcaggact tgtacaaatg tcagaaatac gtgcctcaga ccaaagaccc
     1021 ggtagatccg ctgctgtgca cgcatctgtt cccggcatca aaagaagccg aatccttccg
     1081 cacgctgcgc accaatatcc tgcatcagca caaggaaaat cctttccaga ccatgatggt
     1141 tgtctcggcg actgaatgcg tcgggaagtc gaccgtcacg tcgaacctgg gtattatcat
     1201 cgctcaggcc ggatatcgag tcatcatcgt cgataccgac ttgcgccatc cgacgattca
     1261 caagaagttc cggctcgaga acaacaaggg gttgaccaac ctgctgaccg gcatgccgtt
     1321 cgatgaagtt attcagccga cctgcattcc ccgtttgagc attctcgccg cgggcggtct
     1381 gccgccgaat cctgctgaaa ttctgcagaa cgacggtttc caggaagtca tcaactttct
     1441 gaagacgcag gcggatttcg ttctcttcga taacccgcca ctgaacctgg ttaccgactc
     1501 cctgattctg gccaccaaga ttcagcgtgt gttctatctc gtcggcatcg gtcataccga
     1561 caagaatgag attcgtacca gcttcgagtt gctgaagggt ctgaactgta atgtggcagg
     1621 cctgatctgc aaccggatcc cgccgaacga gatgggtggc tactactact attatgtgaa
     1681 gagctgacct caaccaggct tgcaaagccc ctcgaaaccg gttagtttga accgtttcga
     1741 ggggtttttt cttgttgcac cactctgatc ataatgtctg gaaatcggca ggcagcctgc
     1801 tgcggtctct catcgccctg gccctgctgc tgctgtcgtg cgggccgatt gccgcatcgc
     1861 cggtctgggt ggtgagtcgc gccgaactga atccctg
//
LOCUS       DAORHD010001060         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78563_length_1896_cov_2.287887, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001060 DAORHD010000000
VERSION     DAORHD010001060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78563_length_1896_cov_2.287887"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKO06_18600"
     CDS             <1..636
                     /locus_tag="PKO06_18600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71722.1"
                     /translation="NLVVRDKDGGVVSINPSPIMEKQERFYRDDLSSHPLYISKLLYK
                     LLFLKVAFPTYTEGYWSGAAGDLPVYSSFFVHQELEQRFRQHVDFFQANLRELIDCLR
                     QEGLDSHRILFYCNPHLEMLQTPPLACWKSSIDQVVQSSVERENCQYLRPDESSFTEI
                     RANPTRFYLRGDMHFSPQGLMKFAQSLYPELSHQLITHFQDNTNHDAVNQR"
     gene            1050..1403
                     /locus_tag="PKO06_18605"
     CDS             1050..1403
                     /locus_tag="PKO06_18605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV71723.1"
                     /translation="MRSPKTVDRVRAELGSRRADLLFIDGDHSFEGVTSDLRMYADFV
                     APRGFIVFHDIIGNPAYPEYGVHVLWARIRQEFPQAIEIVYPGPCEGFGIGILQKPDE
                     TCFSLFLDRLQSSTC"
BASE COUNT          444 a          482 c          437 g          533 t
ORIGIN      
        1 aatctggtgg tacgagataa agacggcggt gtggtatcga tcaaccccag tccgatcatg
       61 gaaaaacagg aacgtttcta tcgagatgac ttgtcatcgc acccgctgta catcagcaag
      121 cttttgtaca agttgctgtt tctgaaagtg gcttttccga cctatactga aggttattgg
      181 tcgggcgccg ccggtgattt gcctgtgtac tcgagtttct ttgtccacca ggaattagag
      241 cagagattcc gccagcacgt cgactttttt caggcgaatc tgcgagaact catcgattgc
      301 ttgcggcaag agggacttga ctcccaccgg attctttttt actgcaaccc acatcttgaa
      361 atgctgcaaa ctcctccgct tgcttgctgg aagagttcga ttgatcaagt tgtgcagtcg
      421 tccgttgagc gtgaaaactg tcaatatctg agacccgatg agagcagctt cacggaaatc
      481 agagcgaatc ccacacgatt ctatttacga ggtgatatgc acttttctcc acaaggcctg
      541 atgaagtttg cccagagtct gtatccagag ttatctcacc agttgataac tcattttcaa
      601 gacaacacca atcacgatgc cgttaatcag cgttgacctc aggcaactct ttcaccttgt
      661 ctgggtcaag gtgaaggatg ttgctgaccc tcttttctgg gctttgtctt tcagcgcctt
      721 caaaaagcat gcgcttgcat tgtctcgaca aatcgatagc atcccagaag tctgcagaaa
      781 gtatcgtggg ttcggctggt atcgaagaat tccctttttc cagaatagaa aagagttttc
      841 agatctcatc cgtttctgct cgcagttaaa ccccgatacc acgattgaga tcgggacagc
      901 attcggcgct actctgctgc tctgggcacg ggtgacccaa tcccttgtca tttccattga
      961 tgacagtgat attccgcgca agcgacatcg gcttttccag agttttccgt ggccgaattc
     1021 acagccgcga atctgtccac taccgatgaa ttcgctcccc caagacagtt gatcgagtcc
     1081 gcgcggaact gggttctcgc cgcgcagatc ttctcttcat tgatggtgac cactcattcg
     1141 agggcgtcac gtcagacctc agaatgtacg ctgatttcgt cgcgcctcgt ggtttcatcg
     1201 tgttccatga catcatcggc aatccggctt atcctgagta tggtgtacac gtattgtggg
     1261 ccagaatccg acaggagttt ccacaggcaa tcgagatcgt ttaccctggt ccatgtgagg
     1321 gattcggcat tggaattctt caaaaacctg atgagacctg tttttccttg tttcttgatc
     1381 gactgcaatc gtcaacctgt tgagatattt ggagagatat gcacggaagc actcttgatc
     1441 gatgcgcaag actactattc tctttcttca gtaaaccacg ctatctattg ccgtggcttt
     1501 caaaaagtgt tttgagtcgg aaaagtccgc tcgatctgag attgccctgg ttttcgtttt
     1561 ctgcaattga ttactttcaa catcggaatc tcaagaacga aatcatcttc gagtatggtt
     1621 ctgggggctc aactgtcttt tttgcccaac gttgtcggca cgtcgtttcc gttgaatccg
     1681 atcaatactg ggcccgcctc gttcgttcga aatgccaatc gatgggtctg aacaacgtcg
     1741 aagtgcgttt cgaaactggc tacttcgacc atctcgagaa acatcaccag tccggctttc
     1801 tcgcggccat taaagattat tcaccgacca tcgtcgtcat agatcattat gagacagacc
     1861 tgcaaacgcg accgaccacg tttcaatacg tacaag
//
LOCUS       DAORHD010001061         1895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78586_length_1895_cov_4.158696, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001061 DAORHD010000000
VERSION     DAORHD010001061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78586_length_1895_cov_4.158696"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PKO06_18610"
     CDS             complement(<1..810)
                     /locus_tag="PKO06_18610"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71724.1"
                     /translation="MKNQELFGVYSRFERFVSGYVVAVIRLLPEGYPSVESRLLRVIR
                     GQAFPLAPEAFAKHLEKNSELDSPRDKVIGAMSELLHLLVETVTYLGKEAGRDLAHRP
                     LLFMSEYGEADERAFALLTLARLGHATGREQLPHLLGEWSRTIRWAVLSLLEREGDWH
                     WMSLFLSCLEDGEPEIVRVAVSAIGKSGTVSAAPRVLELLHGSGEAVTIAAALALGRL
                     REPAALLPLMELGANTRSQKVRASVVSALGEFGEPRVVPFLARNLEQRDARV"
     gene            complement(839..>1895)
                     /locus_tag="PKO06_18615"
     CDS             complement(839..>1895)
                     /locus_tag="PKO06_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71725.1"
                     /translation="MKLAQLAESKGDAVRAVGYLDTLHRSLKPAAENLTLVLKIGDLL
                     EDKVKNSELIMLYWDMILRDYAKVPNVRELARGKIRVQTQLRLAAAAKPTGPRKAKRV
                     YTEEDEELLEQIDDIKKRLIDDLEDFVKAERELLILWEENPESLVTYDIMKLLVELNE
                     NQLRDPQKAAEYYDRWLNENPTDSGYDDVVLKLYDLYMEKVRDGQKALRLLENFINTR
                     PNSPMILEVQLKLGKANELLVRNFSEALRVYQRIIDTRRNEPVVHEAFFRTAFVLREG
                     YASYDEAIALWTEMNDLFYNNQFAAEAQFEIGAIYEFYRRDFTKAREAYEKLLNLYPN
                     SPLQNKVREALLRLGVK"
BASE COUNT          401 a          579 c          498 g          417 t
ORIGIN      
        1 cacacgggca tcgcgctgtt ccagattacg ggccaaaaat ggcaccaccc gcggctctcc
       61 gaactcgccg agtgccgaaa ccaccgacgc gcgaactttc tggctgcggg tattcgcacc
      121 cagctccatc agtggcagca gcgctgcggg ttcgcgcaga cggccgaggg caagagccgc
      181 cgcgatggta accgcctcgc ccgacccgtg caacagttca agcacacggg gagccgcact
      241 gaccgtgccg ctcttgccga tggcactgac tgccacgcga acgatttcgg gttctccatc
      301 ttcgagacag gaaaggaaca gactcatcca gtgccagtct ccctcgcgtt caagcaatga
      361 caggaccgcc cagcgaatgg ttcgcgacca ctcgccaagc aggtgcggca gttgctctcg
      421 tccggtcgca tggcccagtc gcgccagcgt taacaaagca aaagcccgtt catcagcttc
      481 gccatactcg ctcatgaaca agagcggacg atgagcgaga tcgcgaccgg cttctttgcc
      541 gagatacgtc accgtctcta cgagcaggtg cagtagttcg gacatggcac cgatcacctt
      601 atcacgcgga gagtccagtt ccgaattttt ctcgagatgc ttggcaaaag cctcaggagc
      661 caatggaaat gcctgcccac gaatcacgcg cagcagacgt gattcgaccg acggatatcc
      721 ttctggcagc agacggatca ccgccacgac gtatccggaa acgaaacgct cgaatcgcga
      781 gtagaccccg aacagttcct ggtttttcat gcttgttcct cccaccggag aactctcatc
      841 acttcacacc gaggcgcagg agagcttctc gcaccttgtt ctgcagcgga gaattcggat
      901 acaggttcaa cagtttttca taggcttcac gggctttggt gaaatcccgg cgatagaatt
      961 catagatcgc cccgatctcg aactgcgcct cagccgcaaa ctgattgttg tagaacaggt
     1021 cattcatttc cgtccacaag gcaatggcct catcgtaact ggcatacccc tcacgcagaa
     1081 caaaggcggt acggaagaac gcctcatgga ccaccggctc gttgcggcgg gtatcgatga
     1141 tacgttgata cacccgtaat gcctcgctga agttgcgcac cagcagttcg tttgccttgc
     1201 ccagtttcag ctgcacttcg agaatcatcg gagaattggg acgtgtatta atgaagttct
     1261 cgagcagacg caacgctttc tgaccgtcac gtactttttc catgtacagg tcgtagagtt
     1321 tgagcacgac atcgtcgtag ccggagtccg tcggattctc gttcagccag cgatcgtagt
     1381 attccgccgc tttctgcggg tcgcgcaact ggttctcgtt cagctcgacc agcagtttca
     1441 tgatgtcgta ggtgaccagg gattcggggt tctcttccca caggatcagc aattcacgtt
     1501 ctgccttgac gaaatcttcg aggtcgtcga tcagacgctt cttgatatcg tcgatctgct
     1561 cgagcagttc ctcgtcttct tccgtataga cacgtttcgc cttgcgcggt cccgtcggct
     1621 tcgccgcagc cgccagccgc aactgggtct gcacgcgaat ctttccacgg gccagttcgc
     1681 gcacattggg aaccttggcg taatcacgca ggatcatatc ccagtacagc atgatcaatt
     1741 cggaattctt gaccttgtct tccagcagat cgccgatctt cagtaccagc gtgagatttt
     1801 ctgccgccgg tttcagactg cgatgcaggg tatcgaggta tccgacggcg cgcacggcgt
     1861 cgcccttgct ttcggccagc tgcgccagtt tcatc
//
LOCUS       DAORHD010001062         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78664_length_1894_cov_3.542686, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001062 DAORHD010000000
VERSION     DAORHD010001062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78664_length_1894_cov_3.542686"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>1894
                     /locus_tag="PKO06_18620"
     CDS             92..>1894
                     /locus_tag="PKO06_18620"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNV71726.1"
                     /translation="MRIILPIKQVPETAAVKMDEKTGTMIRDGVEAIINPLDLYAIEA
                     AIRLRDKHGGEIIAVTMGPPKAMSALREAIAMGVDKAVLLSDKAFAGADTWATSYVIA
                     EAIKKLGIGDLIICGERATDGDTGQVGPGIAAWLDLPLITYVGHIEAKTNGSGLTCEV
                     RREVEDGVEKLTMQLPGVLTVIKEVGDPRLPTLRGKQKARAADVPVWGRAELHLDPKN
                     LGLEGSPTRVVKIFRPKVTRTCEKIAVSDSANAGPAIEKLLSFLSSRRGSGATPTGTD
                     TTGKSRPVSSAAKPTGCGKAITGGIWVLGEQANGRVQRITHELLTRGRALADARGVPL
                     TAVILGHTFDETDLREVGERGADRVLTLEHPDFAEFTCEPFTAGLTWLVEQERPEIII
                     AGATTTGRTVMPCLAIKANTGLTADCTGLEIEPESGNLLQTRPAIGGNIMATIKTPKH
                     RPQMATVRPRSTPPAPRQAGRACQIERRAFPQTKLEKRVGRIGFDARSEEQGLSEAET
                     VVCVGRGIKRGENLPLARRLANGLNGALGATRDVVDRGWLDYPHQIGLSGKTVTPKLY
                     VSIGVSGAIQHLAGMQTAETIIAIDQDPEAQIFKV"
BASE COUNT          403 a          589 c          610 g          292 t
ORIGIN      
        1 caccacatcg tcttcatgca ctggagcata gcagaaagcg gacacctgcg acaagttgac
       61 gagggggaac agaggaagat acagtgacag catgcgtatc atacttccca tcaaacaggt
      121 tcccgagaca gccgcggtca agatggacga gaaaaccgga acgatgatcc gggatggtgt
      181 tgaagccatc atcaatcccc tcgatctgta tgccatcgaa gccgccattc gcctgcgcga
      241 caagcacggc ggcgagatca tcgccgtgac catgggcccg ccgaaagcca tgtcggccct
      301 gcgcgaggcg atcgccatgg gcgtcgacaa ggcggttctg ctcagcgaca aggcgttcgc
      361 cggtgccgac acctgggcca cctcgtatgt tattgccgag gcgatcaaga agctgggaat
      421 cggcgacctg atcatctgtg gcgagcgggc gaccgacggc gataccggcc aggtcggccc
      481 aggcattgcc gcgtggctcg acctgccgtt gatcacgtat gtcggacaca tcgaggcaaa
      541 aaccaacggt tcgggtttga cctgcgaggt gcgccgcgaa gtcgaagacg gcgtggagaa
      601 gctcaccatg cagttgcccg gcgtgctcac cgtcatcaag gaagtcggcg atccgcgtct
      661 gccgacgttg cgcggcaagc aaaaagcccg ggccgccgac gtgcccgtgt ggggcagggc
      721 tgaactccac ctcgacccga agaacctggg gctcgaaggc tcgccaaccc gcgtggtcaa
      781 gatctttcgt cccaaagtca cgcgaacctg cgaaaaaatc gctgtcagcg actctgcgaa
      841 tgccggaccg gcgatcgaaa agctcctctc gtttttgagc tcgcgcagag gttccggagc
      901 cacacccact ggtactgaca cgacaggaaa aagtcgtccg gtatcctccg cggcgaaacc
      961 gaccggatgc ggcaaggcaa tcaccggggg gatctgggta ctgggcgagc aggcaaacgg
     1021 gcgcgtgcag cgcatcacgc acgaactact gactcgcggc cgggctctgg ccgatgcccg
     1081 cggcgtgccg ttaaccgctg tgatccttgg tcacaccttc gatgagacgg atcttcgtga
     1141 agtcggagag cgtggtgctg accgggtatt gacgctcgag cacccggact tcgccgagtt
     1201 cacctgcgag ccgttcaccg ccggcctcac ctggctggtc gagcaggaga gacccgagat
     1261 catcattgct ggcgcgacca cgacaggtcg cacggtaatg ccgtgtctgg ccatcaaagc
     1321 caataccggc ctgacggccg actgtaccgg gctcgagatc gaacctgaga gcggcaacct
     1381 gctgcagacg cgcccggcga tcggcgggaa catcatggca acgatcaaaa cgccgaagca
     1441 tcggccacag atggctacgg tgcgaccacg cagcactccg cccgcgccgc gccaggcggg
     1501 acgcgcctgt cagatcgagc ggcgcgcgtt tccgcagaca aagctggaga agcgggtcgg
     1561 acggatcggg ttcgacgccc gctcggaaga gcagggcctg tcggaagccg agaccgtggt
     1621 ctgcgtcggg cgcggcatca aacgcggcga gaacctgccg ctggcacgcc gactggcgaa
     1681 tggcctgaac ggtgccctgg gtgcgacccg cgacgtggtc gaccgaggct ggctcgatta
     1741 tccacaccag atcggacttt ccggcaagac ggtcacgcca aagctctacg tgagcatcgg
     1801 ggtatcgggg gccattcagc atctggccgg catgcaaacg gccgagacga tcatcgcgat
     1861 cgatcaggac ccggaggcgc agatcttcaa ggtc
//
LOCUS       DAORHD010001063         1893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78723_length_1893_cov_3.513058, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001063 DAORHD010000000
VERSION     DAORHD010001063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78723_length_1893_cov_3.513058"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1893)
                     /locus_tag="PKO06_18625"
     CDS             complement(<1..>1893)
                     /locus_tag="PKO06_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71727.1"
                     /translation="VNPGPINSFLASATTPQPASTSFRLYMEALDQYLNRKTDYSGLM
                     SFFDTHTGGTAAYQPKQLAPASWSTGLATLTPGVSFTKAETVKVAVQGDNRTGQSNDI
                     VIHGATATSLLLAVSTNQPEAGVPFGLTLTSLDTFGNVAINYGGTVGFSCTDIASGAK
                     VPATYTFVPADQGIRSWLASTTLMTPGPVTITVKDTVIASMTKSYPVTVLPGPAARFE
                     ISCSPIVTAGVPFNIVITAYDAYGNKKTNFTETVNLVTTFTSILPSSVSGFSNGELLV
                     PSTEVTHSGALPATGLIRVTFGAGASGSIPITLYPPSSSFSRFDLEAIPASPTAGDAF
                     KLVIKAVGPDGAVYTNYAGVGVTLDASNTSGLEVMPPMLPESATGFTIGVKEVYARIW
                     EAGTITIWAHDQTIPGKVGSLTLTVLPTNLSYYTIVPGTSTVEPYPNPYYQVVNGSFP
                     LYLSAFDSIGNLKTNYTGTVSITENGTGTLTATQATFVGGQATISATIYDQPGKMQIT
                     IHDTILDKLGTSSKISFFGPIASLAISCGTTQTDEAAFLTQVNAYDIYGQLKLNTLAS
                     LSSSAPYWSLAPGVSMNLTPPSLNPVSWVDGVAYPILTANRGDAGADVGTLTFGLRAT
                     STASVQ"
BASE COUNT          396 a          498 c          585 g          414 t
ORIGIN      
        1 tgttgcacgg aagccgtgga tgttgctcgg agaccaaagg tcagggtacc gacatccgcg
       61 ccggcatctc ctcgatttgc cgtcaggatc ggatacgcga cgccgtcaac ccacgaaacc
      121 gggttcagcg agggcggggt cagattcatg ctgacccccg gtgccaggct ccagtagggt
      181 gccgagctgc tgagcgaggc cagagtgttg agtttgagct gaccgtagat atcgtaggcg
      241 ttgacctggg tcagaaaagc ggcttcgtca gtctgcgtcg ttccgcacga aatggccagc
      301 gaggcaatgg gtccgaagaa cgagatcttg ctcgaagtgc cgagtttgtc gagaatagtg
      361 tcgtgaatcg tgatctgcat cttgcccggc tggtcgtaga tcgttgctga gatcgtggcc
      421 tgacccccga caaaggttgc ttgtgttgcg gtaagcgtac cagttccatt ttctgtgatg
      481 cttacggtac ctgtatagtt ggtcttgagg ttcccaatcg agtcgaatgc ggagagatac
      541 agcggaaacg atccattcac cacctggtaa tacgggttcg gataaggttc gacggtcgag
      601 gtgcctggga caatcgtgta atacgacaga ttcgttggca gcaccgtcag cgtcagcgaa
      661 ccgactttcc cgggaatggt ctggtcgtga gcccagatcg tgatcgtccc tgcttcccag
      721 atgcgcgcat acacttcctt gacgccgatc gtaaagccgg tcgccgactc gggcagcatt
      781 ggcggcatga cctcgagacc agaggtattg ctggcgtcga gggtgacacc gacaccggca
      841 taattcgtgt acaccgcgcc gtctggtccg acggctttga tcacgagttt gaacgcatcc
      901 cccgccgtcg gcgaagcggg aatggcttca agatcgaagc ggctgaaaga agacgatgga
      961 ggatacagag tgatcggaat agaacccgat gccccggccc cgaacgtgac tctgatcagc
     1021 cccgtcgcgg gcagtgcgcc gctgtgcgtt acttcagtcg acgggacgag aagttcgccg
     1081 ttcgagaacc cgctcaccga agaaggaaga atcgacgtga aggttgtcac gagattgacc
     1141 gtctcggtga agtttgtttt cttgttgccg taggcatcgt aagcggtgat cacgatgttg
     1201 aaaggcactc cggcggtaac gattggcgag cacgagatct cgaagcgtgc cgctgggccg
     1261 ggcagtacgg tgaccgggta actctttgtc atgctcgcga tgacggtgtc ttttacggta
     1321 atggtgactg gtcccggggt catcaaggtt gtgctcgcca accagcttct gatgccctga
     1381 tcagccggaa cgaacgtgta cgtggctgga acttttgccc cggacgcgat gtcggtgcaa
     1441 ctgaacccaa cagttccacc ataattgatg gccacgttgc cgaaggtatc aagagaggtc
     1501 agggtcagac cgaatgggac tccggcttct ggttgattgg tactgactgc cagcagcaga
     1561 ctggtcgccg tcgctccgtg aatgacgatg tcgttcgatt gtccggtgcg attatcgccc
     1621 tgcacagcga ccttgaccgt ctcggctttg gtgaacgaga cgccaggtgt caaggtagcc
     1681 agacctgtgc tccacgaggc tggcgccaac tgttttggct ggtacgccgc cgtaccgccg
     1741 gtatgcgtgt cgaaaaaact catcaatcca gagtaatctg tcttacgatt caaatactga
     1801 tccagagcct ccatatacag tcgaaaactc gtactggccg gttgcggggt cgtggccgat
     1861 gccagaaagc tgttgatcgg gccaggattg acg
//
LOCUS       DAORHD010001064         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_78810_length_1892_cov_2.848122, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001064 DAORHD010000000
VERSION     DAORHD010001064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78810_length_1892_cov_2.848122"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1892
                     /locus_tag="PKO06_18630"
     CDS             <1..>1892
                     /locus_tag="PKO06_18630"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV71728.1"
                     /translation="LTGSGLASSKGRILLDLLTRFLTTSRLTPAEKSALDSRTSGIFG
                     EHMPFEFLAYQRRGRATPGTFEGNRVFILWDYVKHRERVVGAYLVVIPWQVLHDVRPL
                     HQAIAPDPARKKSPLVPFLIPSSAAPKHLRLSAPPGHEISPAQRDFFRSMTAHLSRDE
                     LVPLGQLVQMRGFLVYRDYLSISLPYEIWVLCPSHVFSKSAPNQLLIVVSILLVNIWF
                     IISAHHLVTGRPLYLPLRAWFPAFIALIGAVPLWLFYEAGSMQIDTSEVRQIQERVDE
                     TSQKLSEVDAGNAAITGFFKNTCRQLLGDLAWYARILSDQSAPFKTAVDQAMQHFSQA
                     GLPLEILLSFPLQGPEKGFTPQGPMPANDSSVRLFTAFMNHCLKVIEVPVPRKADQMS
                     EWMALRAFTGHQGPKGMEMVFGGNNEASMYDSGGRKLLYFQDLLSVKGIPKAFLIFRG
                     LAADGYQHRLQKILATLNSREEPFRNYVLGQAVPGGLEIDVAADQQRFWASTAGRNLE
                     EFMQLAHLNKTRQMYQGHRSFFIVHPCRHADGFFIGAHIPLQDIRLNARANRTFLNCL
                     TILILSFILIIGLVASRRLISPLSYFETALVKVAQGNVTIRLGLDRDDELGLMSRSFD
                     NMIIGL"
BASE COUNT          376 a          556 c          489 g          471 t
ORIGIN      
        1 tctcacgggc tccggtctgg cctcctcaaa aggacggatc ctgctcgacc ttctgacgcg
       61 gtttctgacg accagccgac ttacgcccgc cgagaaaagc gctcttgaca gtcgcaccag
      121 cggcatcttc ggagagcaca tgccttttga gtttctcgca tatcagaggc gcgggcgagc
      181 cactcccggc acctttgaag gaaaccgggt tttcatcctg tgggactacg tcaagcatcg
      241 ggaacgagtt gttggtgcgt atctcgtcgt gattccgtgg caggttctgc acgacgttcg
      301 cccgctccat caagccatcg ccccggaccc ggcaaggaag aaaagtccac tggttccttt
      361 cctcattccc tcgagcgcgg ctccgaaaca cctccgcctg tccgctccgc cagggcatga
      421 gatctctcca gcccaacgag actttttccg ttccatgacc gctcacctat cgagagatga
      481 gcttgtgccg ttgggacagc tcgtacagat gcgtggattt ctcgtctatc gcgactacct
      541 ttctatcagt ctgccctatg agatttgggt tctctgtccg tcacatgtgt tttccaaatc
      601 tgctccgaat caactcctga tcgttgtcag catcctgctg gtcaatatct ggttcatcat
      661 ttccgctcat catcttgtca ccggtcgacc tctctatctg ccgctacggg cctggtttcc
      721 cgctttcatc gctctcatcg gcgctgttcc tctctggctg ttttacgaag ccggttcgat
      781 gcagatcgac acgtcggaag tccgccagat tcaggaacga gtcgacgaga caagtcagaa
      841 gctctcagag gttgatgccg gcaatgccgc catcaccggt tttttcaaga acacgtgccg
      901 ccagttactg ggagatctcg catggtatgc ccgcatcctt tcggaccaga gtgctccgtt
      961 caaaacggcc gttgatcagg cgatgcagca tttctcccaa gctggtctgc cgctggaaat
     1021 tcttctcagt tttcctctgc aaggccctga gaaagggttt actccccaag gtcccatgcc
     1081 tgccaatgac tcgtccgttc gactttttac cgcattcatg aaccactgcc tgaaggtcat
     1141 cgaggttcct gtaccccgca aggctgatca gatgagcgaa tggatggcac tgcgtgcatt
     1201 caccggacat cagggtccga aggggatgga aatggtgttc ggcggcaaca atgaagccag
     1261 catgtatgat tcaggcggga ggaagctgct ctatttccag gatctgcttt cggtgaaggg
     1321 gatacccaaa gcgtttttga ttttccgcgg gttggcggcg gacgggtatc agcatcgact
     1381 tcagaaaatt ctggcgacgc tcaactcgcg cgaggaacca ttccgcaact atgtccttgg
     1441 acaagcggtt cccggcggat tggagatcga tgtcgcggcc gatcagcaga gattctgggc
     1501 gtcaaccgcc ggcaggaacc tggaagagtt catgcagctc gctcacctca ataaaacacg
     1561 ccagatgtac caggggcacc ggtcgttttt catcgttcac ccgtgccggc atgcagatgg
     1621 ttttttcatt ggcgcccata ttccgctgca ggacatcaga ctcaacgcac gcgccaatcg
     1681 cacgtttctg aactgtctga ccattttaat tctgtctttc atcctgatta tcggtctggt
     1741 cgcatcgcgc cgtttgattt ctcccttgtc ctatttcgaa acggcactgg tgaaagtggc
     1801 acaaggcaac gtcacgatcc gcctcggcct cgatcgcgat gacgagctgg ggctcatgag
     1861 tcgttcgttc gacaacatga tcattggtct ga
//
LOCUS       DAORHD010001065         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79038_length_1888_cov_3.321331, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001065 DAORHD010000000
VERSION     DAORHD010001065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79038_length_1888_cov_3.321331"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKO06_18635"
     CDS             <1..775
                     /locus_tag="PKO06_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNV71729.1"
                     /translation="DFTADAEAVRKVINTWVAEQTRDRIKNLLPEKSLDSLTRLVLVN
                     AVYFKGVWAQAFDKAETKPQAFFVDGKESVELPLMFRKMNSGYLKGDDYQALELPYQG
                     NRLGMVVFLPDAKDGLAAFEKKMTGEGLSKLLGMLQHEKDIQVYLPKFTFSWGGGMKE
                     HLQALGMKVPFEDNADFSGMTGKPDLKIDQVYHQAFVEVNEEGTEAAAATAVVMTEKG
                     ISFEIEPKLFRADHPFLFLIRDRETGTVLFMGRVTDPRK"
     gene            842..1540
                     /locus_tag="PKO06_18640"
     CDS             842..1540
                     /locus_tag="PKO06_18640"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNV71730.1"
                     /translation="MKVVERFQIEPEICAYLGDRPSVIEYALVSELTAAEYEDLMNTG
                     WRKFGPLLFHPICRECRECRPIRVAVESFKPSRSQQRNRRDNADLDVRVVLPEATEER
                     VELYNSYHRWRQRSRGWPETSMGLMEYQALAVNNPIRGGEILLRHRGKLIGVMQLDFT
                     THVVSDVYHFYDPAYASRGLGTFLILQTLELARRQQKPWVYLGYHVVGSRSMEYKTRY
                     RPYEILDQEGVWRG"
     gene            complement(1555..>1888)
                     /locus_tag="PKO06_18645"
     CDS             complement(1555..>1888)
                     /locus_tag="PKO06_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71731.1"
                     /translation="PTPRMRPGHHRPWCCRRESERSPAAPGTTNEKAAPRIRCPKCGW
                     QPQAEDRWLCCHRIGPYDACCGHVWNTFDTRGKCPNCHYQWTVTACLSCHQWSAHEDW
                     YESPTTED"
BASE COUNT          389 a          502 c          591 g          406 t
ORIGIN      
        1 cgatttcact gccgatgccg aggccgtgcg taaggtcatc aacacctggg tcgccgaaca
       61 gacccgggac cgcatcaaga atctgctgcc ggaaaagtcg ttggattcgt tgacccgact
      121 ggttctggtc aatgccgtct acttcaaagg cgtgtgggcg caggcgtttg acaaggcaga
      181 gaccaaaccg caagcgtttt tcgtggacgg caaagagagt gttgaattgc cactgatgtt
      241 tcgcaagatg aattccggtt atctgaaagg cgacgattat caggcgctcg aactgccgta
      301 tcaggggaat cggctcggca tggtggtgtt tctgcccgat gccaaggacg gattggcagc
      361 gttcgaaaag aaaatgaccg gagaggggct gtcgaagctt ctcggcatgc tgcagcacga
      421 aaaggacatt caggtctacc ttcccaaatt cacgttttcg tggggcgggg gaatgaaaga
      481 gcacctgcag gcgcttggca tgaaagttcc gttcgaagac aacgcggact tttccggcat
      541 gaccggcaag ccagatctga agatcgacca ggtgtaccat caggcattcg tggaagtgaa
      601 cgaagaggga accgaagccg cggctgccac ggcggtcgtc atgaccgaga aaggcatcag
      661 cttcgagatc gaaccgaaac tgtttcgcgc cgaccacccg ttcctgtttt tgattcgcga
      721 tcgggagacc ggaaccgttc tgttcatggg tcgcgtcact gatccgcgaa agtaagcgat
      781 accgccgccc cgaggttcga ctcgagcctg cggggcggct tctttttcca ctgaacgtgt
      841 catgaaggtc gtcgagcgtt ttcagatcga accggaaatc tgtgcctacc tgggcgatcg
      901 cccttcggtg atcgaatacg ccctggtcag cgagctcacg gcggctgagt atgaagatct
      961 gatgaacacc ggatggcgca agttcggccc tctgttgttt cacccgatct gtcgcgaatg
     1021 tcgggaatgc cggccgatcc gtgttgccgt cgagtcattc aaaccatctc gcagtcagca
     1081 acgaaatcgc cgggacaatg ctgatctcga tgttcgtgtc gtgcttcctg aggccacgga
     1141 agaacgcgtc gaattgtaca attcttatca tcgctggcga caacgaagtc gcggttggcc
     1201 tgaaaccagc atgggactga tggagtatca ggcactggcc gtcaacaacc ccattcgcgg
     1261 tggggagatt ctgctgcgac accggggcaa gctgattggc gtgatgcagc tcgatttcac
     1321 cacgcacgtg gtctcggatg tctatcactt ttatgacccg gcgtatgcct cgcgggggct
     1381 tggcacgttt ctgattctgc agacgcttga gctggccagg cgtcagcaga aaccctgggt
     1441 gtatcttgga taccatgtcg tgggttcgcg cagcatggaa tacaaaacgc gctatcggcc
     1501 gtatgaaatt cttgatcagg aaggggtctg gcggggctga cgcggcgccg ccggtcagtc
     1561 ttccgtggtt gggctttcgt accagtcttc gtgcgcggac cactgatggc aggagaggca
     1621 ggccgtgacc gtccactggt aatgacagtt cggacacttg ccgcgcgtgt cgaaggtgtt
     1681 ccagacgtga ccgcagcagg cgtcgtacgg accgatgcga tggcagcaca gccagcggtc
     1741 ttctgcctgc ggctgccagc cgcacttggg acagcgaatg cggggtgcgg ctttttcgtt
     1801 tgtggtgccg ggagcagccg gtgatctctc ggactctcgc cgacaacacc agggtcgatg
     1861 atgaccgggt ctcatgcgtg gcgtgggg
//
LOCUS       DAORHD010001066         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79385_length_1883_cov_3.603939, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001066 DAORHD010000000
VERSION     DAORHD010001066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79385_length_1883_cov_3.603939"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="PKO06_18650"
     CDS             complement(<1..1732)
                     /locus_tag="PKO06_18650"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV71732.1"
                     /translation="MPRRARADHSTGMPRPAPLATDILAAVPDSLLIMTGAAAAARGA
                     YEAGIGLAVTYPGSPITETFEVLAAAPAASAPVCHLVINEHVAFHKAMGYALSGGRAL
                     VTMKHVGFNVAADPAHYIGYTGVRGGMVILVGTDPGATCSTGEYDFRFYAPHTHLPLL
                     EPRSGQEILDALRQAFEWSEEDELPYVVAVPAGACYALEGVRVGAVQQPKADLRFHNS
                     PDLTNVGRRAVLHHQKLLARERRVRQRAGGRGLVQVDGHGSEAAIITSGYHVSRVREA
                     LLRLGLSERVRVLHSLLTSPFPTDSLARELDGIDRLVFVEDLGGFLEQTLSRALLDLN
                     RTVRVSGKEVFPATGALDLQTVEQGLGQIFGVSREPIALPTPLPEVPEREGTFCPGCP
                     DRAFFFALTEQLGPHDVLGGDIGCSSLPPHFSSWLTCMNSGTAIAAGVTLALNGRDLV
                     VSLIGDSTLLHSGLQTIIEAVATDSDQICFVLDNHWTAMTGHQRTPATPVDAAGQPCS
                     HLDIEQLLRACGVQRLSRADPFHLDAFRALLKRLRQERGFRVVLIQRECGLQERRRPE
                     KPGWKQQFVLD"
BASE COUNT          405 a          610 c          558 g          310 t
ORIGIN      
        1 catcgagaac gaactgctgt ttccacccgg gtttctccgg acgccgacgt tcctgcaagc
       61 cacattcgcg ttgaatcagc accacccgaa atcctcgttc ctgacgtaat cgcttcaata
      121 acgcccgaaa ggcatcgaga tgaaacggat cagcacgcga caagcgctgc accccgcacg
      181 ctcgcagtaa ctgttcaata tcgagatgcg aacacggttg ccccgcggca tcgaccgggg
      241 tcgccggagt gcgctgatgg cccgtcattg cggtccagtg gttatcgagc acgaaacaga
      301 tctgatcaga gtcggtggcc accgcttcga tgatcgtctg caatccgcta tgcagcaggg
      361 tcgagtcacc gatcagcgac acgaccagat cacgaccatt caacgccaga gtcaccccgg
      421 cagcgatcgc cgtgccactg ttcatgcagg tcaaccacga ggaaaagtgc ggtggcaggc
      481 tcgaacagcc gatatctcca cccagcacgt catgcgggcc cagctgttcg gtcagggcaa
      541 aaaagaacgc gcgatccgga caccccgggc agaacgttcc ctcccgttcc gggacttccg
      601 ggagtggtgt cggcaacgca atgggctcgc gagacacgcc aaagatttgt ccgagtccct
      661 gttcgacagt ctgcagatcc aaggctcctg ttgcagggaa cacctctttg ccagagactc
      721 gcaccgttcg gttcaggtcg agcaaagccc gactcaacgt ctgctcgaga aagccaccca
      781 ggtcttcaac gaagaccagt cggtcgatgc catcgagctc tcgcgccaga gaatccgtgg
      841 gaaaggggga ggtcagcagc gaatgcagca cgcgcacccg ttcgctcagt ccgaggcgca
      901 gcaaggcttc gcgcacacga ctgacgtgat accccgaagt gatgatcgcc gcttcgctgc
      961 cgtgcccgtc gacttgtacc agcccccgac ctccggcacg ctggcgcacc cgacgctctc
     1021 gtgccagcag tttctgatga tgcagaaccg cacgacgtcc gacgttggtc aaatcgggcg
     1081 agttgtgaaa acgcagatcg gctttcggtt gctgaacagc accgacccgc acgccttcga
     1141 gggcataaca ggcaccggct ggtacggcga cgacatacgg cagttcatct tcttcagacc
     1201 actcgaaggc ctgacgcagg gcgtcgagaa tctcctgccc tgagcgcggt tcgagcaaag
     1261 gcagatgcgt atgcggcgcg tagaaacgaa aatcatactc cccggtggaa caggtcgctc
     1321 cgggatcggt gccgaccaga atcaccatgc cgccacgaac gccggtgtat ccgatgtagt
     1381 gcgccggatc agccgccacg ttgaaaccga cgtgcttcat cgtcaccagc gcccggcccc
     1441 cgctcaaggc gtatcccatg gccttgtgaa acgccacgtg ctcgttgatg accagatgac
     1501 acaccggcgc cgacgcggca ggagcggccg ccaacacctc gaaggtttcc gtgatggggg
     1561 accccgggta cgtcaccgcc agcccgatac cggcttcgta agcaccccgc gcggcggccg
     1621 cggctcccgt catgatcaac agcgaatcag gcacggccgc cagaatgtcg gtggcaagcg
     1681 gagcgggacg aggcatgccg gtggaatggt cagcgcgcgc gcgacgaggc atgcgtccct
     1741 ttctgacctg cacgagtcga cgtctcgctc tctcgaggag accatgccgc aagtcggcgg
     1801 cgcaacgtct cggggaacgt ggccggtaca ggatcgaggt caaaaagagc cggtgcggcg
     1861 acagcagagc tgtcgctttc aga
//
LOCUS       DAORHD010001067         1882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79439_length_1882_cov_4.846743, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001067 DAORHD010000000
VERSION     DAORHD010001067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79439_length_1882_cov_4.846743"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PKO06_18655"
     CDS             complement(<1..1081)
                     /locus_tag="PKO06_18655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71733.1"
                     /translation="MFHRISVRRFWCLLLSLLLTFEPLILAAEQASGEPQEEEFAVDK
                     ALSPGDYLKNIKELRGENREEIEKRSWADWATLMANVYLLMDPNAIDAPSFENALGET
                     LTDIESVSGFGAALPGRVEKVANILTWGSTHFGKVSPRLAGFSTKLLSGWGHVATKVM
                     PKLDKFTGFCEFMHAPGNAGEAEAFQQWLKRKPPTTEGLAPLSKAQGAAMVVGTVIQI
                     LQGIAEIKSFFDAKSNDPGAASFATVSHAITGTVLIACAIMALIPPPGVWMVVAAVVS
                     VIWDTLKSVFDKVGEKIAKWHKAYEDSLTFLKETDREFSAFLSANDEASVLRTGQEKS
                     AALNLADKMKRALDENPGEGEVEERQ"
     gene            complement(1090..1710)
                     /locus_tag="PKO06_18660"
     CDS             complement(1090..1710)
                     /locus_tag="PKO06_18660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71734.1"
                     /translation="MRLRLIGVACLLLCGVSLLSAGPLSREEQKKLAESLYAEMTKAD
                     EWDLDLFIKNHRRVIDECPETTLAAESCWRLSNLYLTARAKPDRQAAIELMEHLMRQY
                     PNCPLREMAASRYVNTCQAAGEFQKMLQFCQERLAEPKLPKDQVVSYTLWAGEASESL
                     GQMPQAAQYYRVVLQHDPQQTGMLAQAAQLRLSRVESQDQTAPAGK"
BASE COUNT          359 a          591 c          524 g          408 t
ORIGIN      
        1 tctgtcgttc ctcgacttct ccctcgccgg gattttcatc gagcgctcgc ttcatcttgt
       61 ccgccaggtt gagggccgcg gacttttcct gaccggttcg cagcacgctt gcttcgtcgt
      121 tcgccgagag aaaggcggaa aactcgcggt cggtctcttt caggaaggtc agcgaatctt
      181 cgtaggcctt gtgccacttg gcgatcttct cgccaacttt gtcgaagacg ctcttcagcg
      241 tatcccagat cacgctcacg acggcagcca cgaccatcca gacccccgga ggcggaatca
      301 gcgccatgat cgcgcaggcg atcagcaccg taccagtaat cgcgtgcgag acggtggcaa
      361 aactcgccgc gcccggatca ttggacttgg catcgaaaaa cgacttgatc tcagcgatcc
      421 cctgcagaat ctggatgacc gtcccaacga ccatcgccgc tccctgggct ttgctcagag
      481 gagccagccc ttcggtggtc ggaggtttgc gtttgagcca ctgttgaaag gcttcagcct
      541 caccggcatt gccgggcgcg tgcatgaatt cacagaagcc cgtgaatttg tccagtttcg
      601 gcatgacctt cgtggccacg tggccccacc ctgagagcag tttggtggaa aatccggcca
      661 gcctgggcga cactttcccg aagtgggtgc tgccccaggt cagaatgttc gccaccttct
      721 cgacccgccc gggaagcgcg gctccaaagc cactcaccga ctcgatatcc gtgagggttt
      781 cgcccagggc gttttcaaaa ctcggggcat cgatggcatt cggatccatc agcagataca
      841 cgttggccat cagggtggcc cagtcggccc aggatcgttt ctcgatctcc tcgcggtttt
      901 cgccccgcag ttccttgatg ttcttcaggt aatcacccgg cgagagagcc ttgtcgaccg
      961 cgaactcctc ttcctgtggc tcgccgctcg cctgttccgc cgcgagaatc agcggttcga
     1021 aggtcagcag caggctcaac agcaggcacc agaaccgacg aacagagatg cgatgaaaca
     1081 tgcgagtcct cactttccgg ccggagccgt ctgatcttgg gattcgacgc gactcagccg
     1141 cagctgcgcc gcctgtgcca gcatgccggt ctgctgcggg tcatgttgca gcaccacgcg
     1201 atagtattgc gcggcctgcg gcatttgtcc gagtgactct gaagcctcac cagcccagag
     1261 cgtgtatgag acgacctgat ctttcggaag tttcggttcg gccaatcgtt cctgacagaa
     1321 ctgcagcatc ttctggaact cgcccgcggc ctggcacgtg ttgacataac gactggcagc
     1381 catctcgcgc agggggcagt tcggatactg gcgcatcagg tgttccatca gctcgattgc
     1441 ggcctggcga tcgggtttgg cgcgggccgt gagatagaga ttgctgagcc gccagcagga
     1501 ttcggccgcc agcgtcgttt cggggcactc gtcaatcaca cgccgatggt tcttgatgaa
     1561 cagatcaaga tcccactcgt ccgctttcgt catttcggca tacaggctct cagccagctt
     1621 cttttgctct tcacgcgaca agggccccgc gctcagtaac gagacgccgc acagcagcag
     1681 acacgcgaca ccgatcagtc tcagcctcat ggtttccccc ctgaaccgga tttggcaccg
     1741 ctttcaggcg gagccggatt ttgtacaagt tcagcccgaa tcttttcttt aattctggcg
     1801 agtttcttca ggtcagattc aatcacgaac tgctcgagag ccagagccgc aacccgaaag
     1861 cccgggtgat gaaaacgcac tt
//
LOCUS       DAORHD010001068         1877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79771_length_1877_cov_3.258507, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001068 DAORHD010000000
VERSION     DAORHD010001068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79771_length_1877_cov_3.258507"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PKO06_18665"
     CDS             complement(<1..314)
                     /locus_tag="PKO06_18665"
                     /inference="COORDINATES: protein motif:HMM:NF037360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71735.1"
                     /translation="MPKIWLANVEEVFLPRHPDDDWTLIPKTDAMAPRFLFYAEPGDV
                     MLLSCEVPRSFVRYVAQMNGFHHEPLRVMAPACPVSPYSLVESVLDDHQLMDALRKLI
                     CIG"
     gene            complement(474..1424)
                     /locus_tag="PKO06_18670"
     CDS             complement(474..1424)
                     /locus_tag="PKO06_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71736.1"
                     /translation="MTISSAGVELLTPGCLGTCWYGKYHYLEPWVGCEHDCDYCYARF
                     RTPVIEAVTARGTSFSRPALTLPLADLQVQLAAELVRQQVEIVKISRFTDFFSASTLA
                     QGWARAVLEVLVASPVRRVIITTKGAPDAACQEIMSRNPDRFSFNVVAKPAASLGFEE
                     RIPSLDARLDAACRLQRAGVLTTIHMDPLIPGFEDQSPMLEEFVDRLAAQGLKRVMFS
                     LLLLNDSMIALMRERRGATVTDRILEQFEPPSTRPYLPGQEETVYRQPKPEVRASCIH
                     RVSQVLSERGFAFVLCSLKSGRGQEGAAAGCPLCDGKFYA"
     gene            complement(1421..>1877)
                     /locus_tag="PKO06_18675"
     CDS             complement(1421..>1877)
                     /locus_tag="PKO06_18675"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV71737.1"
                     /translation="VTLARRNLSDCPNVEIVHTSFLDVRLPANSYCRGWARKALHHLT
                     DPEKDRFFRSVGPAFQPDSLFLLEDGMFDFARERLEDEWPRVLREAEHYYGDRWAAIR
                     ADVEHTLRHEFPTGAERWAEALRCGGFVPVSVTQRTCFYGAILARKERE"
BASE COUNT          441 a          584 c          530 g          322 t
ORIGIN      
        1 ccgatgcaga tcagtttgcg cagggcatcc atcaactgat gatcgtcgag aaccgactcc
       61 accagtgaat acgggctgac cgggcacgcc ggcgccatca cccgcagcgg ctcgtgatgg
      121 aacccgttca tctgcgccac ataacgcacg aagctgcgtg gcacctcaca ggacagcagc
      181 atgacatcgc ccggttctgc gtaaaacaga aaacgcggcg ccatggcatc ggtctttgga
      241 atgagcgtcc agtcatcgtc ggggtgacgt ggcagaaaca cctcttcgac gttggccaac
      301 cagatcttag gcatacgaaa cccacacggc aacacgtttt cccagcataa gacagccgat
      361 tccttcaagc cacacaggaa ctgtggtcat agtaagatga actcccgaat cgagagcaac
      421 agaaaacaga aatcacgaaa aaatgcaagt cctctctcct ctactctacc attttatgcg
      481 tagaattttc catcacagag cggacaacct gccgccgccc cttcctgccc gcgtcccgac
      541 ttgagcgaac acagcacgaa ggcgaacccc cgctcggaga gaacctgact gacgcgatgg
      601 atgcaggatg cgcgcacctc gggtttcggc tggcgataga ccgtttcctc ctggccggga
      661 agatacgggc gcgtcgacgg cggctcaaac tgctcgagga tacggtcggt cacggtcgct
      721 ccccgacgtt cacgcatcag agcaatcatg gagtcgttca acagcagcag cgagaacatc
      781 acccgtttca ggccctgagc cgccagccgg tcgacaaatt cttcgagcat cggtgactga
      841 tcttcaaagc cgggaatcag gggatccatg tggatggtgg tcagcacccc cgctcgctgc
      901 aggcgacagg cggcgtccag gcgcgcatca agcgacggaa tgcgttcctc gaagccgaga
      961 ctcgcagccg gcttggcgac gacattgaag gagaaccggt cggggttccg gctcatgatc
     1021 tcctggcagg cagcatctgg tgcgcccttg gtggtgatga tcactcgacg caccggagaa
     1081 gcgaccagca cctcgagcac cgcccgcgcc cagccctgcg ccagggtcga agcactgaag
     1141 aaatccgtaa aacgcgaaat cttcacgatc tcgacctgct gccgcaccag ttcggcagcc
     1201 aattgaacct gcagatcagc cagcggcagt gtcaacgcag gccgagagaa ggacgtgccc
     1261 cgagccgtga ccgcctcgat gacgggagtg cgaaaccggg catagcagta atcgcagtca
     1321 tgctcgcacc caacccaggg ctcaagataa tgatattttc cgtaccagca ggttccgagg
     1381 catcccggcg ttaaaagctc aactccagcg gaagaaatgg tcattccctt tcctttcgcg
     1441 ccaaaatcgc tccgtagaaa caggtccgct gcgtgaccga gaccgggaca aatccgccac
     1501 agcgaagcgc ttccgcccag cgctctgccc cggtgggaaa ctcgtgtcgc agagtatgct
     1561 caacatcggc gcgaatggcg gcccagcggt caccataata gtgttcggct tctcgcagca
     1621 cgcgtggcca ctcgtcttcg agccgctcgc gggcaaagtc aaacatgcca tcttcgagca
     1681 ggaacagact gtcaggctgg aaagccgggc ccacggagcg aaagaatcta tccttttcgg
     1741 gatcggtgag gtgatgcagc gcctttctcg cccagccccg acagtaggaa ttggcgggga
     1801 gacgcacatc gagaaatgag gtgtgcacga tctcgacatt ggggcagtcg ctgagattcc
     1861 ggcgtgccag cgtcacc
//
LOCUS       DAORHD010001069         1875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79889_length_1875_cov_2.978022, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001069 DAORHD010000000
VERSION     DAORHD010001069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79889_length_1875_cov_2.978022"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..>1875
                     /locus_tag="PKO06_18680"
     CDS             304..>1875
                     /locus_tag="PKO06_18680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71738.1"
                     /translation="MQFVTRRWFALFLALACLIPALEARQLPTAISTDNDALHDETIA
                     VCTPVTKGKGNFFTNVLGKALNLPKTVKIEDLGFQFMALSMEAFQATPADPKSPVVVK
                     NLPYLKASKEIQDSMKLRRTARQIRAQLMAELLKKADQLNDEQKKALNSFLTQSVGAP
                     VIILDKSPLPSFMPTPGPCMIPIGIERVEAGTEKRIGLETSAICLLDIPGVFSVSEAQ
                     ANDTLDIVLAHENAHAIQFDMMGKGFQLINRPSTNGHDAPIISDLGLAYTEGWAEAFE
                     AVYGPANPKFKEKDRKKFNISEFLYARQDPIRRERYVWSKVGGKQTGLAKNGLQLMST
                     EGVIAGLFYDILTSRKITAPFEKCVSVMLMQQPKNFPEFVLSYLRMFKDDRAVVLRIV
                     LENTHYSVCSNEARKAYQAWYLARTAYQQKKGSQEDASKAEKAYRTLKEELFAKAYKS
                     DAEIFTAVGPDLWFSGEVKLEKKISALDVKAQIMKKLGQDPDVFKFNLNLNTINRGML
                     KFIGVAEADADMIVTK"
BASE COUNT          460 a          560 c          483 g          372 t
ORIGIN      
        1 gccccgcaag ggcaaaaagc tgtaccagca gttgcccaag ctcatcgacg cgctcaagac
       61 cgagtatcat gccgtgctca agaaacaggc gaaaggcaag aaatagcaga acgaccgctt
      121 tcggacggcc cggcgagctt ctcgtccggg ccgttttttt cgaccacttt gccctcctgt
      181 tcttcgccac cccgattcga ttttcatcag agaaacggct gtagaagcga cacatacccc
      241 ttgcgggtac actgtttcgc cagtatgatt aaaaaatatc cctgccggca aaggagaatt
      301 tcaatgcaat ttgtaacacg ccgctggttt gctctcttcc tcgccctggc ttgtctgatt
      361 cctgcgctcg aagcgcgtca gctgcccacg gcgatctcca ccgataacga tgcgttgcat
      421 gacgagacca tcgccgtctg cactccggtc accaaaggga agggcaattt tttcacgaac
      481 gttttgggga aagctctcaa cctgcccaag accgtaaaaa tcgaagatct cggcttccag
      541 ttcatggctc tctcgatgga agcgttccag gcaactccgg ctgacccgaa gagcccggtc
      601 gtggtcaaga accttcccta tctgaaggcc agcaaagaaa ttcaagattc gatgaagctg
      661 cgccgcaccg cccggcagat tcgcgcacag ctgatggcag aactgctgaa gaaggctgac
      721 cagctgaacg atgagcagaa gaaggccctg aactcgttcc tgacacaatc ggtcggcgct
      781 ccggtcatca ttctcgacaa gagcccgctc cccagcttca tgcccacacc cggcccctgc
      841 atgattccaa tcgggatcga acgcgtcgaa gccggcacgg aaaagcgcat cggccttgaa
      901 acttccgcca tctgccttct cgacattccc ggcgtcttct cagtgagcga agctcaagcc
      961 aacgacaccc tcgacatcgt tctggcgcac gaaaacgcac atgccatcca attcgatatg
     1021 atgggcaagg ggttccaatt gatcaaccgg ccgtcgacaa acggccacga cgcgccgatc
     1081 atcagcgatc tgggcctcgc ctataccgaa ggctgggcag aagcgtttga agccgtgtat
     1141 ggccccgcga accccaagtt caaggaaaaa gaccgcaaga agttcaacat ctccgaattt
     1201 ctgtatgccc gccaggatcc gatccgtcgc gaacgctacg tctggtcgaa agtcggcggc
     1261 aagcagaccg gtctggccaa gaacggcctg cagctgatga gcacggaagg cgtcattgcc
     1321 ggtctgttct acgatattct caccagccgc aagatcaccg ctcccttcga gaagtgcgtt
     1381 tccgtcatgt tgatgcagca gcccaagaac ttccccgagt ttgtcctgag ttacctgcgc
     1441 atgttcaagg atgatcgcgc cgtcgtgttg cgcatcgtgc tcgaaaacac gcattactcc
     1501 gtgtgcagca atgaagcccg caaggcgtat caagcgtggt atctcgcgcg caccgcgtat
     1561 cagcagaaga aagggtcaca ggaagacgcc agcaaggccg agaaagccta tcgcacgctg
     1621 aaagaagaac tgtttgccaa ggcgtacaag agcgatgccg aaattttcac ggctgtcggc
     1681 cctgacctgt ggttctctgg tgaagtcaag ctggaaaaga agatcagtgc tcttgatgtg
     1741 aaggcccaga tcatgaaaaa gctcggccag gacccggacg ttttcaagtt caacctgaac
     1801 ttgaacacaa tcaatcgtgg catgctcaag ttcatcggcg tcgccgaagc tgatgccgac
     1861 atgatcgtca ccaag
//
LOCUS       DAORHD010001070         1874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_79966_length_1874_cov_2.997251, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001070 DAORHD010000000
VERSION     DAORHD010001070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79966_length_1874_cov_2.997251"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PKO06_18685"
     CDS             complement(<1..823)
                     /locus_tag="PKO06_18685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71739.1"
                     /translation="MLKRIALLTAVLFVFMTTASFAGDLMKLIPADSAFVLHLNLTKI
                     LANENVKKSIDENMAKQSPEQKKAYDEFIQKTGIDPLKSITELMVFVATTGDKGKPDA
                     AVLFNGTFDKNKIMEAIKADKKAAEETIIEKFEGFDAIKSKKADEGTGVFLDDTTAVV
                     GTANPIKTVIDVKNGKAKDLGTNEKFVNLMKKIDTAANLWGAGVIPEELKAQAKNNPQ
                     AAPLANVNTVCFSFNFDKDITFNFMGEVDKKENMDQVMTALNGFLAMIKMVAGQTP"
     gene            1011..>1874
                     /locus_tag="PKO06_18690"
     CDS             1011..>1874
                     /locus_tag="PKO06_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV71740.1"
                     /translation="MPQNPPTDIMTRRSNPRPEDIRGPYLRDQTAIIHSLPFRRLKHK
                     TQVFFAPENDHICTRIEHVMHVATIAATIARGLNRSGWKLDEDLAFAIGLGHDLGHAP
                     FGHSGEAILDDLLKPFGQRFVHEVNGLRVVDALANDGEGLNLTFAVRDGIICHNGEKF
                     EPALQPAERANVPELICDRNCRPSTFEGCIMRFADKIAYLGRDIEDGIDAGLIKESDI
                     PDRIRQRMGSRNGEIIHTLVIDVIETSRKADAIRLSDETFSLMRELKTFNYQSIYLHP
                     SLKEYESYCRNI"
BASE COUNT          400 a          511 c          514 g          449 t
ORIGIN      
        1 ccggagtctg gccagcgacc atcttgatca tggccaggaa accgttgagg gcggtcatga
       61 cctgatccat gttttccttc ttgtcgactt cacccatgaa gttgaaagtg atgtctttgt
      121 cgaagttgaa ggagaagcac acggtgttga cattggccag aggagcggcc tgcggattgt
      181 tcttggcctg ggccttcagc tcttcaggaa taacgccagc gccccagagg ttggcggcgg
      241 tgtcgatctt cttcatcagg ttaacgaact tttcgttggt gcccagatct ttggctttgc
      301 cgttcttgac atcgatgacg gtcttgatcg ggttcgccgt gccgacgacg gcagtggtgt
      361 catcgaggaa cacgccggtg ccttcatcag ccttcttgga cttgatggca tcgaaacctt
      421 cgaacttctc gataatggtt tcttcggcgg ctttcttgtc ggccttgatg gcttccatga
      481 tcttgttctt gtcaaaggtt ccattgaaca gcacggcggc gtcgggtttc cccttgtcgc
      541 cggtggtggc cacgaacacc atgagttcgg tgatgctctt cagaggatcg atgccggtct
      601 tctgaatgaa ttcgtcatag gctttcttct gttccggact ctgcttggcc atgttctcgt
      661 cgatggactt cttcacgttt tcgttggcca gaatcttggt cagattcagg tgcagaacaa
      721 aagcggaatc ggcgggaatc agcttcatga gatcgccggc aaaagaagcg gtcgtcatga
      781 acacaaacag aacagcggtc agcagggcaa tgcgtttcaa catagggatc cattctcctt
      841 atttttagcg ggttagcagg aaggatctcc ctgctgattt cgccgaatca tacccggggt
      901 gtaccgcgaa gtcaagaaaa atgcagaaag cgcgaaatca ctcgccgcct gcgggccccc
      961 tatggtacga tcacacaaca gtcaactctc gcattcaagg cggtctcagt atgccacaga
     1021 atccacccac cgatatcatg acccgacgaa gcaacccccg tccggaagat attcgcggac
     1081 cctacctgcg tgaccagaca gccatcattc actcgctgcc gtttcggcgc ctcaaacaca
     1141 agacacaggt attcttcgct cccgagaatg atcacatctg cacccgcatc gagcatgtca
     1201 tgcatgtcgc gaccattgcc gcgaccatcg cccgagggtt gaaccgctcg ggctggaagc
     1261 tggacgagga tctcgcgttt gccatcggtc ttgggcacga tctcggacat gcgccgttcg
     1321 gccattccgg cgaagccatt ttagacgatc tgctcaagcc ctttggccaa cggttcgttc
     1381 acgaggtgaa cggtctgcgt gtcgtcgacg ccctggcgaa cgacggcgag gggctgaatc
     1441 tcacgttcgc cgtacgcgac ggcatcatct gtcataacgg cgagaagttt gaaccagctc
     1501 tgcaaccggc tgaacgggcc aacgtgccgg aattgatctg cgaccgcaac tgccgcccct
     1561 cgacgttcga aggctgtatc atgcggtttg ccgacaagat tgcctatttg gggcgtgata
     1621 tcgaagatgg gatcgatgcc gggctgatca aggaatcgga cattcccgat cgcattcgcc
     1681 agcgcatggg cagtcggaac ggcgagatca ttcatacgct ggtgatcgac gtgatcgaaa
     1741 cttcgcgtaa agccgacgcg atcaggttgt ctgacgagac gttctcgttg atgcgtgaac
     1801 tgaagacctt caattatcag tcgatttatc tgcacccgag cttgaaggaa tatgaatcgt
     1861 actgtcggaa catt
//
LOCUS       DAORHD010001071         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_80391_length_1868_cov_3.257584, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001071 DAORHD010000000
VERSION     DAORHD010001071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80391_length_1868_cov_3.257584"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PKO06_18695"
     CDS             <1..827
                     /locus_tag="PKO06_18695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71741.1"
                     /translation="IRFLQVRVHPLLGGKGILGKVVVLDDFTMLKTIERRMFEGEKWV
                     SLGKLAASVAHEIRNPLVAIRGLVEMIGEDLSGENARHVRVVIGEVNRLNRVVEELLQ
                     LTRPEKAELKQTSLAELLDELLLLVRHEAERHRVVIRKEWSDGPFHVTIDPEKVKQAF
                     INVMLNAIQAMPQGGTLTISLKQTPWPGLPEEDDRHAVAIEFADTGVGIQRELVDKVF
                     DPFFTTRPQGTGLGLSITRKVVDLHQGQIKLHSEPGHGTRIQILLPNHPDTQGGLP"
     gene            824..>1868
                     /locus_tag="PKO06_18700"
     CDS             824..>1868
                     /locus_tag="PKO06_18700"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV71742.1"
                     /translation="MNPPAVPTAARILLVDDEESVLYTLEAVLSREGYALTRAKCVTE
                     ALSRLDEASFDLVISDVCMPGESGLDLLDEVRRRDPEGLVLLITAFGSETLAVDAMKR
                     GAYDYLPKPFANDDLKITVRRALERRRLRVENLQLRERLQEREGLHAIIGSSEGMQRV
                     YDLIEKVAPNDVTVLITGESGTGKELVATAIHALSPRKNAPFVRVNCAALPENLIESE
                     LFGYERGAFSGAVNRRLGKFELADKGTIFLDEIGDMSLTTQTKILRILQEREFERLGG
                     QSSVKVDVRVIAATNRDLSKAMKENQFREDLFYRLNVVSIQIPPLRDRRSDVPALVEH
                     FAGGFRKKFCKPDW"
BASE COUNT          398 a          491 c          576 g          403 t
ORIGIN      
        1 atatccgttt cctgcaggta cgggtgcatc cgctgcttgg cggcaagggt attctgggaa
       61 aagtcgtggt gctcgatgat ttcaccatgc tcaaaaccat cgaacggcgc atgtttgagg
      121 gagaaaagtg ggtgtcgctg ggcaaacttg cggcttcggt cgcccatgag attcgcaatc
      181 ctctggtcgc cattcggggc ctggtggaga tgattggcga agatctgtct ggtgagaatg
      241 cccggcatgt ccgagtggtc atcggtgaag tcaatcgact caaccgggtg gtggaagaat
      301 tgctgcagtt gacccgccct gagaaggcgg agctgaaaca aacctctctg gcggaactgc
      361 tcgatgaact cctgctgttg gtcaggcatg aggcggaacg tcatcgggtg gtgatccgca
      421 aagagtggtc tgatgggccg tttcatgtta caatcgatcc cgaaaaagtg aagcaggctt
      481 tcatcaacgt gatgctcaat gccattcaag ccatgccgca gggcggaaca ctgaccattt
      541 ccctgaagca gaccccctgg ccgggtctcc ccgaagaaga cgatcgccac gcggtggcga
      601 ttgagttcgc ggatacggga gtcggcattc agcgtgaact ggtggacaag gtgttcgacc
      661 cgttcttcac caccagaccc cagggaaccg gactcggact ttccattacc cgcaaagtcg
      721 ttgatctgca tcaggggcag atcaaactgc actcggaacc cggtcacgga acccgcatcc
      781 agatcctgtt gccgaatcac ccggataccc agggaggtct gccatgaatc ctccggccgt
      841 tccaaccgcg gctcgcattc tgctcgtcga tgacgaagag tcggtactat acacgcttga
      901 ggccgtattg agtcgtgagg ggtatgcgtt aacccgggcg aaatgcgtga ccgaagcgtt
      961 gtcgcgactc gacgaggcgt cgttcgatct cgtgatctcg gatgtatgca tgccgggtga
     1021 atcagggctc gatctgcttg acgaagtgcg gcgtcgtgat ccggaaggcc tggtacttct
     1081 gattaccgcc ttcggcagcg agacgctggc cgtcgatgcc atgaaacgtg gggcctacga
     1141 ctatcttccc aagcctttcg ccaatgatga tctcaaaatc acggttcggc gggccctcga
     1201 gcgccggcgt ctgcgggtcg agaacctgca gttgcgcgag cgtctgcagg agcgcgaagg
     1261 attgcatgcg atcatcgggt cgagcgaagg catgcagcgg gtctacgacc tgatcgaaaa
     1321 agtggccccc aacgacgtga ccgtgctgat cactggtgag tctggtaccg gaaaggaact
     1381 ggtggcgacg gctattcatg cgctcagtcc gcgaaagaat gcgccttttg tgcgcgtcaa
     1441 ctgtgccgcg ttgcccgaaa atctgatcga gagcgagttg ttcggctatg agcgcggcgc
     1501 tttcagcggt gcggtcaacc gtcgtctggg caaattcgaa cttgccgaca agggaacgat
     1561 ttttcttgat gagatcggcg acatgtcgct gaccacgcag accaagattc tgcgaattct
     1621 gcaggaacgt gagttcgagc gtctgggtgg gcaatcgagc gtgaaagtcg atgttcgagt
     1681 gattgccgcg accaatcgtg acctgtccaa ggcgatgaag gaaaaccagt ttcgggaaga
     1741 cctcttctat cggttgaatg tcgtcagcat ccagattccg cctttgcgcg atcggcgctc
     1801 agacgtgccg gctctggtgg aacattttgc cggaggattt cggaaaaagt tctgcaaacc
     1861 tgactgga
//
LOCUS       DAORHD010001072         1867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_80450_length_1867_cov_3.437086, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001072 DAORHD010000000
VERSION     DAORHD010001072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80450_length_1867_cov_3.437086"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..1138)
                     /gene="pheS"
                     /locus_tag="PKO06_18705"
     CDS             complement(125..1138)
                     /gene="pheS"
                     /locus_tag="PKO06_18705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNV71743.1"
                     /translation="MSESIETLWREFEAALPESQNSKDLENLRIKYLGKKGPMAQLMK
                     ELGQLAPVDRPAMGSKINALKERITQKLEEAIEKIGGAEQQAKIAAERVDLTLPGVVQ
                     QPGAMHPVARVAQEVASIFLSMGFFLADGPEVEQEYYNFEALNIPKYHPARDMQDTFY
                     VENGKVLRTQTSPVQIRTMEQIKPPLKMIALGKVFRRDSDITHSPMFHQMEGLVVGEN
                     VRMSDLKGTLIMFAERLFGKRPFRFRPSYFPFTEPSAEMDIHCVFCGGKGCKVCKMSG
                     WMEVLGCGMVNPRVFQAVNYDAEKYTGFAFGMGIERLAMQRYGIDDIRLLFGSDIRFL
                     QQF"
     gene            complement(1203..>1867)
                     /locus_tag="PKO06_18710"
     CDS             complement(1203..>1867)
                     /locus_tag="PKO06_18710"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV71744.1"
                     /translation="IREARASGLELETLLTERPAHWRQPEWADRLCELKSFLMKRLSR
                     LETPPPDIGLFRTRRLPPLAQALRAARTVLVLDRVQDPGNLGTIIRSMEALAADLLIL
                     LPGCCSPVNDKVLRAAMGSAFRLPVAVVATDDEALKTLAEQGFTLVATAMNGTSITGC
                     SFPEKIALILGAEGRGVRPEILAQAALQLTIPIREPVESLNVAMAATICLYERMRQRN
                     GK"
BASE COUNT          358 a          579 c          516 g          414 t
ORIGIN      
        1 ggctgcgata tcgcctcgac ctcgatcccg accatcagca gcgcgtcggc aatgcgctcc
       61 ggcgtttctt gaatgtcaat atagtcacga agccagctca tcagaatttt catggtgtgt
      121 ctccttagaa ctgctgcagg aagcggatat cggatccgaa cagcagccgg atatcgtcga
      181 ttccgtagcg ttgcatggcc agacgctcaa tccccatccc gaatgcaaac ccggtatatt
      241 tctcggcgtc gtagttcacc gcctgaaaca cgcgcgggtt caccatgcca cagcccagaa
      301 cctccatcca gcctgacatc ttgcacacct tgcagccttt tccgccgcag aatacgcagt
      361 gaatgtccat ttccgccgac ggttccgtga acggaaagta gctggggcgg aatcggaacg
      421 ggcgcttgcc gaacagacgc tcggcgaaca tgatcagcgt gcccttcagg tcgctcatgc
      481 gcacgttttc accgacaacc agaccttcca tctggtggaa catcggcgaa tgcgtgatgt
      541 cgctgtcacg gcgaaagact ttcccgagag cgatcatctt cagcggcggc ttgatctgtt
      601 ccatcgtgcg aatctgcacc ggcgaggtct gcgtgcgcag caccttgccg ttttcgacat
      661 agaaggtgtc ctgcatgtcg cgcgccgggt gatacttggg aatgttcagc gcttcgaagt
      721 tatagtattc ctgctcgacc tcggggccat cggccagaaa gaagcccatc gacagaaaga
      781 tcgaagccac ttcctgcgcc acccgggcga ccggatgcat ggctccgggt tgctgcacga
      841 cgccgggcaa cgtcagatcg acacgctccg cggcaatttt cgcctgctgc tccgcgccac
      901 cgattttctc gatcgcttct tcgagctttt gcgtgatgcg ctctttgagc gcattgatct
      961 tggaacccat ggccggtcga tccaccggtg ccaactgtcc gagttctttc attaactgcg
     1021 ccatcggacc tttcttgcca agatacttga tgcgcaggtt ctccagatct ttcgagtttt
     1081 gcgactcggg cagagccgcc tcgaactccc gccacaacgt ttcgattgat tcgctcatgt
     1141 attcagcctt tcacgggaaa gcccgtcaat gttttttctt ttctaactac ctgttattct
     1201 ctctatttgc catttctctg tctcatgcgc tcgtacagac agatggtcgc ggccatggcc
     1261 acgttcaacg attcgaccgg ttcccgaatc ggaatcgtga gttgcagggc ggcttgcgcc
     1321 agaatctccg ggcgcacgcc acgtccttcg gccccgagaa tcagcgcgat cttttcagga
     1381 aatgagcagc cggtgatcga cgtgccgttc atggcggtcg ccaccagcgt gaacccctgc
     1441 tctgccagtg ttttcagtgc ctcgtcatcg gtcgcgacca ccgcgacggg cagtcggaat
     1501 gcggacccca tggcggcacg cagcaccttg tcgttgaccg gtgaacagca acccggcaga
     1561 aggatcagca gatccgccgc cagggcctcc atgctgcgga tgatggttcc caggttcccg
     1621 gggtcctgaa cgcgatcgag cacgagaacc gttcgcgccg cgcgcagcgc ttgcgccaac
     1681 gggggcaggc gacgggtgcg aaagaggccg atatccggcg gaggtgtttc cagacgcgag
     1741 agtcgcttca tcagaaacga tttcagttcg cagagtcggt ccgcccattc gggttgtcgc
     1801 cagtgtgccg ggcgttcagt cagcagcgtt tcgagttcga gccccgaagc tcgcgcctcg
     1861 cgaatca
//
LOCUS       DAORHD010001073         1863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_80761_length_1863_cov_2.373341, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001073 DAORHD010000000
VERSION     DAORHD010001073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80761_length_1863_cov_2.373341"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..995)
                     /locus_tag="PKO06_18715"
     CDS             complement(276..995)
                     /locus_tag="PKO06_18715"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HNV71745.1"
                     /translation="MAYISLYFFAAFLVGSIPVGELICRLTGKGSLFVPGEWGPLRIG
                     GVFEKLGRPLGLVVTLIDALKGWFVVWPLITIVFNDPNYHEYWYIVSGGGLAVVIGHC
                     HSLFLGFRGGRGAATTSGVMFALMWQPTLAAMVVWGVCAYWALSTRPGTLISAGALPI
                     FCLIWIWVFRPEDSLYLYVVAALAIWTVFEHRITLLRYLGLLPEQQLPIVLTPRATDS
                     KGAASATQTSEPNPETEQKNS"
     gene            complement(1025..>1863)
                     /locus_tag="PKO06_18720"
     CDS             complement(1025..>1863)
                     /locus_tag="PKO06_18720"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNV71746.1"
                     /translation="GAILAFAAQKFAVDEDPRIGEVTALLPGANCGGCGFPGCAGLAK
                     AIVVDSVEPNLCPVASAEAREKIAKHLGRAAVASKPKVALVKCNGLPAEEFKKFEYLG
                     IQDCAAAVLMLNGPWQCPHRCIGLGTCKAVCPFGAITMGANELPVIDAEKCTACGKCV
                     IACPRMIITLVEKGKKVHVKCNSPEKGPDVRKVCKVGCIGCRLCEKACKDGAIIIENN
                     LARINYEKCTECGACVLKCPQKTITMVGGVPVAAGASQPVVPPPAVSAPTSTGSQPSK
                     PA"
BASE COUNT          444 a          585 c          482 g          352 t
ORIGIN      
        1 ggtgctcatt actccacgag ccccgtttac cggcgtcgaa acccaggcac gcagttttct
       61 cctgcagctg tttttgcagg gagccgacct gtcacagttc gcgacggagt ttgcccccgg
      121 actcatgcgc attaagttcg tcggagcaaa accggccacc ccctgaaatc gcacgcgggc
      181 aaaagatggc tgcctgtttt cctgttcaca aaaagaaaac gtcccggatc aaccaggacg
      241 ctctcgaatc aacaattcga agaccaatca aactgtcagg aattcttttg ctctgtttcc
      301 ggattcggtt cgctcgtttg agtggcagac gccgctcctt tcgagtccgt ggcacgcggg
      361 gtaagaacaa tcggcagttg ctgttccggc aacaacccca ggtatcgcag caacgtaatg
      421 cgatgttcga acacggtcca gatcgccagt gccgcaacga catacaggta cagactgtct
      481 tcggggcgaa acacccagat ccagatcaga cagaagatgg gcagcgcacc ggcggaaata
      541 agcgttcccg gccgggttga gagagcccaa taggcgcaca ctccccagac caccatcgcc
      601 gccagcgtcg gttgccacat caaagcaaac atgactccgc tggtcgtggc agcaccacgg
      661 cccccgcgaa accccagaaa cagcgaatga caatggccga taacgaccgc caagccgcca
      721 ccactgacga tataccagta ttcatgatag ttcggatcat tgaacacgat tgtgatgagc
      781 ggccagacaa cgaaccagcc tttgagcgcg tcgatcagag ttaccacgag accgagtggt
      841 cgcccgagtt tctcgaagac accgccaatg cgcagtggtc cccattctcc cgggacaaaa
      901 agactgccct tgcccgtgag tcggcagatc agttcaccga ccggaattga accgaccaga
      961 aaagccgcaa aaaagtacaa actgatatag gccacagaaa tctcgctttg gattcaatga
     1021 gttctcaggc cggcttcgat ggctgagaac cggtcgaagt cggggcggaa accgcggggg
     1081 gaggaaccac cggctgcgaa gcaccggcag cgaccggaac cccgcccacc atggtgatgg
     1141 tcttctgagg gcacttcaac acgcaggctc cgcactcagt gcacttttcg tagttaatgc
     1201 gcgccagatt gttctcgatg atgatcgcac catccttgca cgctttctcg caaagacggc
     1261 acccgataca cccgactttg cagacttttc gaacgtccgg gcccttttcg ggtgaattgc
     1321 acttcacatg cacttttttg cccttttcga ccagcgtgat gatcatgcgc ggacaggcga
     1381 tgacgcattt tccacatgcg gtgcactttt ccgcatcaat gacgggcagt tcattcgcac
     1441 ccatggtgat ggccccgaac ggacagaccg ccttgcacgt accaagaccg atacagcgat
     1501 gcgggcactg ccacggacca ttcaacatca gaaccgccgc cgcacaatct tgaatgccaa
     1561 gatattcgaa cttcttgaac tcttccgccg gcaggccgtt gcacttcacg agagccactt
     1621 tcggcttgct cgcgacagcc gcacgaccca gatgcttggc aatcttctct cgtgcctccg
     1681 cagaggcgac ggggcacagg ttcggctcca cactgtcgac cacgatcgcc ttggcaaggc
     1741 cagcgcaacc gggaaagccg cacccaccgc aatttgcgcc cggcagcaac gcggtcactt
     1801 cgccaatgcg cgggtcttca tcaacggcaa acttctgcgc ggcaaacgcg agaattgcac
     1861 cga
//
LOCUS       DAORHD010001074         1858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81055_length_1858_cov_3.341098, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001074 DAORHD010000000
VERSION     DAORHD010001074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81055_length_1858_cov_3.341098"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PKO06_18725"
     CDS             <1..376
                     /locus_tag="PKO06_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011135341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNV71747.1"
                     /translation="DQNTGEILDRNALNDELEKIEKPAGISNPKDFRNEVVNFVLRAR
                     AKNNGKNPSWTSYEKLRAVIEKKMFSNTEDLLPVISFNAKASGDDQKKHQNFVNRMIE
                     KGYTERQVHLLCEWYLRVRKSS"
     gene            413..1678
                     /locus_tag="PKO06_18730"
     CDS             413..1678
                     /locus_tag="PKO06_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNV71748.1"
                     /translation="MVRIIDRRFDSKNKSTVNRQRFMRRFKKQIREAVADAISGRSIK
                     DLDNGESVSIPAKDLSEPQFHHGHGGVWETVFTGNDQFSTGDQINRPQGGGGGTGKGK
                     ASKDGEGEDDFVFNLSREEFLDVFFDDLALPDLVKTQLARIEEYKSQRAGFTNDGTPA
                     NINVVRSLRGALGRRLALASPWQGKLRELQRQLDELLETRPDNDPDVTRLKDEIGALR
                     ARIEAIPFIDTFDLRYNNRIKVPKPTTQAVMFCIMDVSGSMDEEKKSTAKRFFMLLYL
                     FLMRTYEHIDLVFIRHHTVAKEVNEEDFFHSRESGGTVVSSAIDLMREILHSRYASGA
                     WNIYGAQASDGDNWENDSPHCRELLAETILPFVQHFAYIEITRGEPQNLWREYEKLPG
                     AHPNFAMQHIEGLPDIYPVFRELFRKKLA"
     gene            1675..>1858
                     /locus_tag="PKO06_18735"
     CDS             1675..>1858
                     /locus_tag="PKO06_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNV71749.1"
                     /translation="MSSPIRAKRKKPLPAPGEWSFELIETYHAEIDRVAREYKLDTYP
                     IQVELITSEQMMDAYSS"
BASE COUNT          461 a          575 c          494 g          328 t
ORIGIN      
        1 cgaccagaac accggcgaaa tccttgatcg caacgcgctc aacgacgaac tggagaagat
       61 cgaaaaacct gctggaatca gcaatccgaa agatttccgc aatgaagtgg tgaacttcgt
      121 gctgcgtgcc cgcgccaaaa acaacggcaa gaacccgagt tggaccagct acgaaaaact
      181 gcgtgcggtg atcgaaaaga agatgttctc caataccgag gatctgctgc cggtgatcag
      241 cttcaatgcc aaagccagtg gcgacgacca gaagaaacat cagaacttcg tcaaccgcat
      301 gatcgaaaag ggttacaccg agcggcaggt tcatctgctg tgcgagtggt acctgcgcgt
      361 ccgcaagtcg tcctgactca gcggcagcac gccaacagtt cgggaggctt gaatggtccg
      421 gatcatcgac cgccggttcg acagcaagaa caagagcact gtcaaccggc aacggtttat
      481 gcgtcgcttc aagaagcaga tccgcgaagc cgtggccgac gcaatttcgg gccgctcgat
      541 caaggatctg gataatggcg agagtgtttc cattcccgcc aaggatctgt ccgagcccca
      601 gtttcaccac ggccacggcg gcgtttggga aaccgtattc accggtaacg accagttttc
      661 caccggtgac cagatcaacc gcccccaagg cgggggcggt ggcacgggca aaggcaaggc
      721 cagtaaggac ggtgaaggcg aggatgactt cgtgttcaac ctgtcccgcg aagaatttct
      781 tgacgtcttc ttcgacgacc tcgccctgcc cgaccttgta aaaacgcagc tcgcccgcat
      841 cgaggaatac aaaagccagc gtgccggctt taccaacgac ggcacaccgg ccaacatcaa
      901 cgttgtgcgc tcgttacggg gcgccctcgg ccgcagactg gccctcgctt cgccctggca
      961 aggcaagctg cgggagcttc agcggcaact ggacgaactc ctcgaaaccc ggcccgacaa
     1021 cgacccggac gtcacccgac tgaaggacga gatcggtgcg ctgcgcgccc ggatcgaagc
     1081 gatccccttc atcgacacct tcgacctgcg ctacaacaac cggatcaagg tgcccaaacc
     1141 aacgacccag gcggtgatgt tctgcatcat ggacgtatct ggctcgatgg atgaagaaaa
     1201 gaagtcgact gcaaaacgct tcttcatgct gctctatctt ttcctcatgc gcacctacga
     1261 gcacatcgat ctggtcttca tccgccacca tacggttgcc aaggaagtaa acgaagaaga
     1321 cttctttcac tcgcgagaat ccggcggcac cgtggtttcg agcgcaatag atctgatgcg
     1381 tgaaattctc cactcccgct acgcgagcgg cgcctggaac atctatggcg cccaggcatc
     1441 cgacggcgac aactgggaga acgactcgcc ccattgccgc gaattgcttg ccgagaccat
     1501 cctgcccttc gtacagcact tcgcctacat cgaaatcact cgcggcgaac cccagaatct
     1561 gtggcgtgaa tacgaaaaac tgcccggtgc ccatccgaac ttcgcgatgc agcacatcga
     1621 aggcttgccg gatatctacc cggtgttccg tgaactcttc aggaagaaac tggcatgagc
     1681 tcaccgatcc gagcgaaacg aaaaaaacct ctcccagccc ccggcgaatg gagcttcgaa
     1741 ctcatcgaga cctaccacgc cgagattgac cgggtcgccc gcgaatacaa gctcgacacc
     1801 tatccgatcc aggtcgagct gatcacatcc gagcagatga tggacgccta ctcgtcgg
//
LOCUS       DAORHD010001075         1857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81114_length_1857_cov_3.801332, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001075 DAORHD010000000
VERSION     DAORHD010001075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81114_length_1857_cov_3.801332"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..366)
                     /locus_tag="PKO06_18740"
     CDS             complement(88..366)
                     /locus_tag="PKO06_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV71750.1"
                     /translation="MPVGSALICRAGCGACCIAPSISSPLPLHPQGKPAGMICRHLTV
                     DRHCELFGRPERPTVCASLKPSSEMCGTSHEQAMAHLNQLEFLTRPDR"
     gene            complement(366..743)
                     /locus_tag="PKO06_18745"
     CDS             complement(366..743)
                     /locus_tag="PKO06_18745"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HNV71751.1"
                     /translation="MIISTTDCIEGHPAEQYLGVVTGEAVFSVGMFSELKAGLKADIS
                     GGRVDSFTKELEAAKDQALVEMVKEAKILAAHAVVGVDIDYLEFRDKYLLVIMSGTAV
                     RLKDFKEAEFPENYMMPTEKAEK"
     gene            complement(827..1138)
                     /locus_tag="PKO06_18750"
     CDS             complement(827..1138)
                     /locus_tag="PKO06_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71752.1"
                     /translation="MGQLIPLFGRTKKTDALPPQPPVSGDASSRLKLVINSLEAHKKE
                     EQALQNEIMKKCHAEIDRLNELDRKIRDLEQEASHLCRNLGQPIPGFGETARKRPDGL
                     L"
     gene            1236..1415
                     /locus_tag="PKO06_18755"
     CDS             1236..1415
                     /locus_tag="PKO06_18755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HNV71753.1"
                     /translation="MNQDQTHVSTSTTNGMLRQSTLWFLVLGIIFTLIGIYVGEPRSI
                     FDKATQICLQCIGIG"
     gene            1412..>1857
                     /locus_tag="PKO06_18760"
     CDS             1412..>1857
                     /locus_tag="PKO06_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004064701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV71754.1"
                     /translation="MSSVTNGISRLWVQAVATLLTNGYLKGFVYGKIFVGPTKHVCVP
                     TLNCYSCPGALFSCPIGAMQVVFASGGGLDVSLYSAFWQRLLTIVSGFPFFVVGLLTI
                     IGSLVGRAACGWICPFGFLQDLLHRIPSRKWRGPAWLSYGKYVVLLV"
BASE COUNT          369 a          497 c          531 g          460 t
ORIGIN      
        1 gcgacgccgc aagctcgaac gctggtttgc gcagtttgtt gaagagatcc atcccgacct
       61 gtagttgttg cgtcctggtg agaacgctca acgatccggt cgggtcaaga attccagctg
      121 attgagatgt gccattgcct gctcatgcga tgtgccacac atttcggaac tcggtttcag
      181 gcttgcacag acggtggggc gctcaggtcg gccgaacagt tcgcagtgcc ggtcgacggt
      241 gagatgtcga cagatcatgc cggccggctt tccctgcgga tggagtggca gtgggctgga
      301 gatagaagga gcgatgcagc aggctccgca tccggcgcga cagatcaagg ctgaaccaac
      361 cgggattact tctcggcctt ctcggtgggc atcatgtagt tttcgggaaa ctcggcttcc
      421 ttgaagtctt tcagacgaac agccgtgccg ctcatgatga ccagcagata cttgtcgcga
      481 aactcaagat agtcgatatc gacacccacg acggcatgag ccgccagaat cttggcttct
      541 ttgaccattt cgaccagagc ttgatccttg gcagcttcca gttccttggt gaagctgtcg
      601 acgcgaccgc cggaaatgtc ggcttttaaa ccggctttca gttcagaaaa catgccgacg
      661 ctgaaaacgg cttcaccggt cacaacaccg agatactgct cggcgggatg tccttcaata
      721 cagtccgtgg tggaaatgat catgtctgcc tctccttcac gtgaacgaat agctgcgtcg
      781 aatcagtgta acatcgtctc aatcagggtg acaagaaacg tttcgatcag agcagaccat
      841 caggtcgctt gcgcgcggtt tcgccgaaac cgggaatggg ttgcccaaga ttgcgacaga
      901 gatgagaggc ttcctgttcc agatcgcgaa tcttgcgatc aagctcgttg agtcgatcaa
      961 tctccgcgtg gcatttcttc atgatctcgt tctgcagagc ctgctcttcc tttttgtggg
     1021 cttcgagcga gttgatgacg agcttgagtc ggcttgatgc atcaccggaa acggggggct
     1081 gaggcggaag agcgtcggtc ttttttgtgc gcccaaagag aggaatgagt tgtcccatcg
     1141 cgtttgccat cctttcgtct caaatctttc cctatgtatc ggcaactttg gtctgcattg
     1201 ccaacccgat tggcgctgga gtatcatgcg ccatcatgaa tcaggatcaa acccacgttt
     1261 caacctcgac cacgaacgga atgctgcggc aatcgaccct ctggttcctg gtgctgggga
     1321 tcatcttcac gttgattggc atctacgttg gcgaaccgcg cagcatcttt gataaagcca
     1381 cacagatctg cctgcagtgc atcggaatcg gatgagcagc gtcacgaacg gcatttcgag
     1441 actctgggtt caggccgtgg cgaccctgtt gaccaacggc tatctgaaag ggttcgtata
     1501 cggaaaaatc ttcgtcggac cgacgaagca tgtgtgcgta ccaaccttga actgctacag
     1561 ttgcccggga gcgttgttct cgtgcccgat cggggccatg caggtcgtgt ttgcttcggg
     1621 cggcgggctt gatgtcagcc tgtattccgc tttctggcag cgactattga ccatcgtctc
     1681 cggttttccc tttttcgtcg ttggtctgct gacgattatc gggtctctcg ttgggcgtgc
     1741 cgcctgtggc tggatctgcc cattcggctt cctgcaggat ctgctgcacc gcattccgtc
     1801 acggaaatgg cgcggtccgg cctggttgag ctacggcaag tatgtggtgc tgctggt
//
LOCUS       DAORHD010001076         1856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81195_length_1856_cov_3.642421, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001076 DAORHD010000000
VERSION     DAORHD010001076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81195_length_1856_cov_3.642421"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKO06_18765"
     CDS             complement(<1..188)
                     /locus_tag="PKO06_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HNV71755.1"
                     /translation="MSLRINQNVLAIRTQGQLAQTSNRLEKSVEKLSSGLRINRASDD
                     ADGLAISEKLRRQIRGLSR"
     gene            complement(601..>1856)
                     /locus_tag="PKO06_18770"
     CDS             complement(601..>1856)
                     /locus_tag="PKO06_18770"
                     /inference="COORDINATES: protein motif:HMM:NF012903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV71756.1"
                     /translation="GSAAFVSISNLALLPNPLGVANGRYTGNSGYAGSTKGTNIITDF
                     DFGATPVTFTITDFHSATTATTINLPSSYAAGGMTSLVDAINTQIVANNLLVRAFNNN
                     GTLELRSLESGVESNFTVVDNLNTLARLFINTQTTNSGYDGVEARQDFAYDQSSAKWG
                     YNIGDSTASAGLDHMSFYIADLDGRGMVITITEGVAALGQSFRTIQSIANIINQEAST
                     AGVKVAAEIITANMSMKIYSTVPGKTGRVTFAELASPDSVNTLNSIFGITPKTYDNGV
                     GQYSYTMHLKDAFIQLQIGPNEGHSAKANIVRTDIVALGIRDLDLTTVRSSQEAIGLV
                     DKALQYISSERAKLGAIENRLTYTTNSLRVGLENMSASESRIRDVDVAREVVELTRFQ
                     ILQQSSNAMLAQANTSAQRVLDLLR"
BASE COUNT          417 a          486 c          495 g          458 t
ORIGIN      
        1 cgactcaatc ctcgaatctg acggcggagt ttttcactga tggccaaccc gtctgcatcg
       61 tcggaggccc tgttgatgcg aagtccgctg ctgagttttt cgacggactt ctcaagtctg
      121 ttagaggtct gggctagctg gccctgcgta cgtatagcga ggacgttttg attgatgcgt
      181 agcgacataa tcgattcctc cctgaatcga aacggaattg gagcttccct gctccatgcg
      241 tgacgcctgc tgtatgaaaa tgttagttct ctaggcaacg ccctcctgat cagatttcag
      301 aaccgttagc gccgaacaaa cagaatcccc cgattcttcc actatataca tcggcaaaaa
      361 gtggcgagaa tcgggggacg catttggccg gttgtacgcc agcccaaacg ggtgacgttg
      421 taccggagga ctatttgatt gtagtataac taactttcgt gatttttcgt gcgttttcag
      481 ggcgaggcaa aggtccttga acagtttttt gctctattgt ctcttagatg acctatcgac
      541 gaaaaaagtt gcgcgcttga gaagtatttt ttcaactttt ttcaagagcc ctcaaaaccg
      601 ctaccggagc agatccagca ctcgctgcgc cgaagtattc gcctgggcca gcatggcatt
      661 gctcgactgc tgcagaatct ggaaccgggt cagttcgacc acttcgcgcg ccacatcgac
      721 atcgcgaatg cgcgattcag aagcactcat gttctcgaga ccgacgcgca ggctgttggt
      781 ggtgtaggtc agacgattct cgatcgcacc aagttttgca cgttcagaag agatgtattg
      841 cagcgccttg tcgaccagac caatggcttc ctgtgaggaa cgaaccgttg tcaggtccag
      901 atcgcgaatg cccagagcta cgatatccgt gcggacaatg ttcgctttgg cactgtgccc
      961 ctcattcggt ccgatctgca actgaatgaa cgcatctttt agatgcatgg tataggaata
     1021 ctgacccaca ccattgtcat aggtctttgg agtaatccca aagatagagt tcagtgtgtt
     1081 caccgagtca ggactcgcca actcggcgaa ggtgacacgc cctgtcttgc caggaactgt
     1141 tgagtagatc ttcatcgaca tgttcgccgt gatgatttca gcggcaacct tgaccccagc
     1201 cgtgctcgct tcctggttga tgatgttggc gatgctctga atggttcgga aactctgtcc
     1261 gagagcggca accccttcgg tgatggtaat gaccatgcca cggccatcga gatcggcgat
     1321 atagaacgac atgtggtcga gacccgccga tgccgtgctg tcgccaatgt tgtagcccca
     1381 cttggctgaa gattggtcgt aggcaaagtc ctggcgagct tcgacaccgt cataacccga
     1441 gtttgtcgtc tgtgtgttaa tgaacagccg cgccagcgtg ttgaggttgt caacaacggt
     1501 aaagttcgac tccacaccgc tttccagcga acgtaactcg agagttccgt tgttattgaa
     1561 agcgcgcacc aacagattat tggccacaat ctgcgtgttg atggcatcta ccagagaggt
     1621 catgccacca gccgcatatg aactgggcag gttaatcgtg gtcgccgtcg tcgcactgtg
     1681 gaaatccgtg atcgtgaacg tgaccggcgt tgccccaaag tcgaagtcgg tgatgatatt
     1741 ggtgcccttc gtcgagcccg catagccgga atttcctgtg taccgaccat ttgccacacc
     1801 cagcggattt ggcagcagtg ccaggttgga aatcgagaca aacgcggcag agcccg
//
LOCUS       DAORHD010001077         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81262_length_1855_cov_3.902222, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001077 DAORHD010000000
VERSION     DAORHD010001077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81262_length_1855_cov_3.902222"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1855
                     /locus_tag="PKO06_18775"
     CDS             <1..>1855
                     /locus_tag="PKO06_18775"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HNV71757.1"
                     /translation="VGLKTGWGLGVAITACILSYAIWNMMRSVVPQIATTQMSILENN
                     CMQSTASSAGYSTGGTMTSAIAAYLLVTGVHMSWPVLAAWTFCLAALGVFMAIPMKRQ
                     MINIEQLRFPSGVAAAETLKSLHAEGGDAVDKARSLTFAGIVGAIIAWFRDAGKPFSI
                     PGMFEIPGSIMGLPLLRWTIGFEMSAIMIAAGAIMGWKIAWSLLLGGVLNYAVVAPWI
                     VEKGAIEAVNLHPDVLLAPFFGFGLSFPLSFDVIRDTAGQVAAAFWVPFSDLASILEL
                     EKCGLRTWVNNICIPLLRENGGLGALKLGYKTIVSWSTWTGASIMVSAGLLAFAFQWK
                     TVIRAFGGLFDVFSAKSTNKDDPLAAIEVPSSWFLGGIALSGLGCLLVLHFAFETSIF
                     MGIVAVLATFFLSVVACRATGESDITPVGAMGKITQLVFGVLAPSNITTNLMTASVTA
                     GAAGSAADLLTDLKSGYLLGANPRKQFIAQFMGIFAGTLVVVPAFYLLVPSADVLGGD
                     KWPAPSAQVWAAVAKLLANGLGALHETARWGILIGVGIGIALTLLEMFLPKSLRPFVP
                     SAMGIGLAMVIPFFNSLSMFLGAVLSIAIMHWYPKQGEKYIVPVSSGVIAGE"
BASE COUNT          291 a          502 c          606 g          456 t
ORIGIN      
        1 cgttggcttg aagacgggtt gggggcttgg agtcgccatt accgcctgca ttctttcgta
       61 tgccatctgg aacatgatgc gatcagtggt gccgcagatt gcgaccactc agatgtcgat
      121 tctcgaaaac aactgcatgc aatcgacggc gtcttcggcg gggtactcaa cgggcggaac
      181 gatgacttcg gcgatagcgg cctatctgct ggtcaccggg gtacatatgt cgtggccggt
      241 gctggccgcc tggaccttct gcctcgcggc tctcggggtg ttcatggcga ttccgatgaa
      301 gcgacagatg atcaacatcg agcaattgcg ctttccgagt ggggtagcgg ctgccgagac
      361 gctgaagagt ctgcacgcgg aggggggcga tgccgtcgac aaagcacgtt ccctgacctt
      421 tgccggaatc gtgggggcga tcattgcctg gtttcgcgat gccggaaagc cgttttccat
      481 ccccggtatg tttgagattc ccgggtcgat catggggctg ccgctgctgc gttggaccat
      541 cgggttcgag atgagcgcga tcatgattgc cgccggtgcc attatggggt ggaagattgc
      601 ctggtcgctg ctgctgggtg gagtgctcaa ttacgcggtg gttgctccct ggattgtcga
      661 gaaaggcgcc atcgaggcgg tcaatcttca tccggacgtg ctgcttgccc cgtttttcgg
      721 gtttggcctg agttttcccc tgtcgtttga cgtgatccgc gataccgccg gacaagtcgc
      781 cgcggctttc tgggtgccgt tttccgatct tgcgtccatt ctcgaactgg agaagtgcgg
      841 gctcagaacc tgggtgaaca acatctgcat cccgttgttg cgcgagaacg gcggactggg
      901 agctctgaag ctcggttaca agacgatcgt gagctggagc acctggaccg gcgcttcgat
      961 catggtttcg gcggggttgc tggcgtttgc ctttcagtgg aagaccgtga ttcgcgcctt
     1021 tggcggcttg ttcgatgtgt tttccgcgaa atccactaac aaggatgatc ctctcgcggc
     1081 gatcgaggtg ccctcgagct ggttcctggg tggaattgcg ctgtcggggc tcggctgtct
     1141 gctggtgctg cactttgctt ttgagacatc gatcttcatg ggaattgtcg cggttctggc
     1201 gacgttcttc ctgtcggtcg tggcctgtcg cgcgaccgga gagtcggata tcacccccgt
     1261 tggcgctatg ggaaagatca cgcagctggt gttcggggtt ctggctccgt cgaatatcac
     1321 tacgaacctg atgacggcaa gcgtcacggc aggagctgcc ggatcagcag ccgacctgct
     1381 gaccgacctg aagagcgggt atctgctggg cgcgaacccg cgaaagcagt tcattgccca
     1441 gttcatgggc atcttcgctg gtacgctcgt ggtggttccg gcgttctatc tgctggtgcc
     1501 gtcggcggat gtgctcggcg gtgacaaatg gccggcgccg tccgcgcagg tgtgggccgc
     1561 ggtggcgaag cttctcgcca acggcttggg ggctctgcac gaaaccgcgc gttggggcat
     1621 tctcatcggc gtcgggatcg gtatcgccct gactcttctc gagatgttcc tgccgaagtc
     1681 tctgcgcccc ttcgtgcctt ctgccatggg catcggtctg gcgatggtca ttccgttttt
     1741 caactccctc tcaatgtttc ttggagcggt gctgtcgatc gccatcatgc actggtatcc
     1801 gaagcagggg gaaaaataca tcgtccctgt cagttcgggc gttatcgcgg gtgag
//
LOCUS       DAORHD010001078         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81563_length_1851_cov_3.495546, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001078 DAORHD010000000
VERSION     DAORHD010001078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81563_length_1851_cov_3.495546"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PKO06_18780"
     CDS             <1..910
                     /locus_tag="PKO06_18780"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV71758.1"
                     /translation="LRARQQNPVRRFLLLVFSAFLLFPCLALAETEKVKCGFSSTTLS
                     NIDARDAEVALRVWLEQILKNEGVTWEFTTRVYDTTAELYDAYNEGNIDVAAVSTMEY
                     LHLAEKKQLEGEFTTEISEKSMETMQMLVRGDSGYQKLEQLQGKRLIIDTRLAGENPV
                     LWLCLELKKRGLPKADAFFRNVTVVKDPSKSALPVFFKTADACIIREQGLQTLAELNP
                     QIGKQLAPILTSPPYIRGVIAFRRGFDPDKRETLRRSALKLHQSARGQQVLTLFKADR
                     VIPLKPEHLLTTIQLRDECRAMKIEP"
     gene            993..>1851
                     /locus_tag="PKO06_18785"
     CDS             993..>1851
                     /locus_tag="PKO06_18785"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV71759.1"
                     /translation="MTDTTPREQTHRQGRGGLFFRTVALTFLVVVLAVGLFLALVTPL
                     LRETLKKSLQSHGEVLASSIAQVTITSIVSEDFSTVVDHCVKVLSDQPPIRFIVISRK
                     DGFSLIHHPDGWSQENSMAWKAIGMDRPIGMIESNRLGTATERVFRYSAPLVYSGIEW
                     GWIHLGISLDSYSRDLAGINQRLLLLCLFSVSAGLLFAWYFADKLVRPIRGLEETARK
                     IGQGELTVRADIHTGDELQSLAETFNLMAETLGRSLDDLRSAKNQAESASRAKSEFLA
                     NMSHEIRTPL"
BASE COUNT          392 a          507 c          545 g          407 t
ORIGIN      
        1 gcttcgtgct cgtcagcaga acccggtcag acgtttcctg ctgctggtct tctcggcttt
       61 cctgctgttt ccgtgtctgg cgcttgccga gacggagaag gtgaagtgcg ggttctcgtc
      121 gacgacgctt tcgaatatcg atgcccgaga tgccgaagtg gctctgcgcg tgtggcttga
      181 gcagatcttg aagaacgagg gcgtcacgtg ggaattcacc acccgggtgt acgatacgac
      241 cgcggaattg tacgatgcct ataacgaggg aaacattgat gtggccgcgg tgtcgaccat
      301 ggagtatctt cacctggcgg agaagaagca actggaaggt gagttcacga cggaaatcag
      361 tgaaaagtcc atggagacga tgcagatgct cgtgcgcggt gattcgggct accagaaact
      421 cgagcagttg cagggaaaac gattgatcat cgatacgcgc ctggccggcg agaatccggt
      481 gctgtggctg tgcctcgaac tgaaaaaacg cgggttgccg aaagccgacg ccttctttcg
      541 caacgtgacg gtcgtgaaag atccttccaa atcggcgttg ccggtctttt ttaaaaccgc
      601 ggatgcctgc attattcgcg aacagggctt acagactctg gccgagttga atcctcagat
      661 cgggaagcaa ctcgctccga ttttgacttc tccgccctat attcgcggtg tcatcgcgtt
      721 tcggcgtggc ttcgacccgg acaaacgtga aacgttgaga cgttctgcgc tgaaactgca
      781 tcaatcggcg cgtgggcagc aggtactgac actgttcaaa gccgaccgcg tcattcccct
      841 gaagccggaa cacctgctca cgacgattca gctgcgtgac gaatgccgtg ccatgaaaat
      901 tgagccgtga gttgtctgcc cgacttcgag aatgtggtgt atcgagagcg gttgtgagag
      961 aatagaagaa ataggggagg atggcacgga ggatgactga cacgacgcca cgcgaacaaa
     1021 cacatcgaca gggccgggga ggattgttct ttcgaaccgt cgccctgacg tttctggtcg
     1081 tcgtgctggc ggtcggtttg tttctcgctc tcgtcacgcc cctcttgcgt gagacgctca
     1141 aaaagagcct gcagtctcac ggcgaagtgc tcgcgagttc gatcgcgcag gtcaccatca
     1201 cttcgatcgt ctccgaagac ttcagcacgg tcgtcgacca ctgtgtcaag gtgctctcag
     1261 atcagcctcc gatccgcttt atcgtgattt cccgcaagga cggattttcg ctgattcatc
     1321 acccggatgg ctggagccag gaaaactcga tggcgtggaa agcgatcggc atggatcggc
     1381 cgatcggcat gatcgaaagt aatcgactcg gcaccgccac cgaacgcgtc tttcgttact
     1441 cagcacctct ggtgtattcc ggtatcgaat ggggctggat ccacctgggc atctcgctcg
     1501 attcgtactc tcgcgatctt gccggtatca accagcgcct gctgttgctt tgcctcttca
     1561 gcgtctctgc cggtttgctg ttcgcctggt acttcgccga caagctggtc aggccgattc
     1621 gcggtctcga agagacagcc cgcaagatcg ggcaggggga actgacggtg cgcgctgaca
     1681 ttcacaccgg tgatgaactg cagtccctgg cagagacgtt caatctgatg gcagaaacgc
     1741 ttggtcgcag cctggacgat ctgcgttccg cgaaaaacca ggcagaatcc gcaagccggg
     1801 cgaaaagcga gtttctcgcc aacatgagcc acgagattcg caccccgctc a
//
LOCUS       DAORHD010001079         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81701_length_1849_cov_3.689521, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001079 DAORHD010000000
VERSION     DAORHD010001079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81701_length_1849_cov_3.689521"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PKO06_18790"
     CDS             <1..1379
                     /locus_tag="PKO06_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71760.1"
                     /translation="SVPRVARIVDQEYRMFDLMASAQEFQSAESQNPVVSNLLQQANE
                     ETRRLRFPDEHRRQKLVSRLLWALFIALIVANLLAFLPWERILPARGPVNASENGMDD
                     GTGRSENSEQPGNSGKNDEGESDTGSDGTQGENGSAGDAKNGEKKDGKCQDGTNGKPG
                     ENGSEGKNGNRGKSGEGTQNGKDGKPGESGNGKSGNGGQSKSSGNQGTQKALNAFGKG
                     LQQNQTTQKLGQALAQNDFQKGAREAEKLAQQPDGKGDAPAQQRQSLAQAAQQALERE
                     GQGADQATQQMLRQLANAGASEARRKEALRQMSQMLKKLAEQQKADANGGQQGTPPKT
                     GAETQAQAKPGQSSGQSPQQQQGVGGTKAGGQLDEDPLQRMQKNRPSQPGDDRLVDPN
                     EQRQVAASAAQRVGAFQGRPELTPEQQQQRQRVAEKAAQSRKVDEFAQYHRIPDEFRT
                     YLKDFFTR"
     gene            1384..>1849
                     /locus_tag="PKO06_18795"
     CDS             1384..>1849
                     /locus_tag="PKO06_18795"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV71761.1"
                     /translation="MLFDLKFLRSLRLLHIMTRRHFRGEKIGHRKSLDRGVSVEFAEY
                     KDYHPGDDLRFIDWNLYGRLEKLYVKKFHSEEDLQVSIFLDTSASMGFGSPSKFDHAR
                     RLAAGIAFIAFQNQDAARLQVFADGLNTATESGYRPGHIHRLMGFLEKLQPSG"
BASE COUNT          484 a          469 c          570 g          326 t
ORIGIN      
        1 tgagtgtgcc cagggtcgca cgcatcgtcg accaggaata ccgcatgttt gatctcatgg
       61 cgtcagcaca ggagttccag agcgctgaat cgcagaatcc ggtcgtgtca aacctcctgc
      121 aacaagccaa tgaagaaacg cgccgcttgc ggtttcccga cgagcatcga cgacagaaac
      181 ttgtttctcg tctgctctgg gccctgttca tcgctctgat cgtcgccaac ctgctcgcgt
      241 ttcttccgtg ggaacgaatt ctgccggcgc gagggccggt gaacgcttct gaaaacggca
      301 tggacgacgg caccggtcgt tcggagaact cggaacaacc cggaaactcg ggcaagaacg
      361 atgaagggga aagcgatacc ggatctgatg gcacgcaggg tgaaaatggt tcggccggtg
      421 atgcaaagaa tggtgagaag aaggatggca agtgccagga tggaacaaat gggaagcctg
      481 gcgagaacgg atctgaaggg aaaaacggaa accgtggaaa gagcggcgaa ggcacccaaa
      541 acggaaaaga tgggaaaccc ggggagagcg ggaacggaaa atccgggaac ggtggtcagt
      601 cgaaaagttc cggcaatcag gggacccaga aggctttgaa tgcgtttggc aaaggcttgc
      661 agcagaatca gacaactcag aagcttgggc aagcgctcgc tcaaaatgat tttcagaaag
      721 gggcccgtga agcggaaaaa ctggctcaac agcctgacgg gaagggtgat gccccggcgc
      781 agcagcgcca gagccttgct caggccgctc agcaagcgct cgaacgcgag ggacagggtg
      841 ccgatcaggc aacgcagcaa atgttgagac aactggcaaa tgccggggcg agtgaagcca
      901 gacgaaaaga ggcgctgcgc cagatgtccc agatgctgaa gaagcttgca gagcagcaga
      961 aagctgatgc taatggcggg caacagggga ccccaccaaa aacaggggct gagactcagg
     1021 cccaggcaaa acccgggcaa tcgtccggcc agagtcctca gcaacagcaa ggtgtcgggg
     1081 gtacgaaggc aggtgggcaa cttgatgaag atccgctgca aagaatgcag aagaaccgcc
     1141 catcgcagcc cggagatgat cgcctggtcg atccgaacga gcaaagacag gtcgccgctt
     1201 cagccgccca gagggttggt gcctttcagg ggcgtccgga actcacaccg gaacaacagc
     1261 agcaacggca acgagttgcg gaaaaagcgg cgcaaagccg aaaagtggat gagtttgccc
     1321 agtatcatcg cattcctgac gagttccgca cgtatctgaa agattttttc acgcggtgag
     1381 ttcatgctgt ttgatttgaa gtttcttcgc agtctgcggt tgctgcacat catgaccaga
     1441 aggcattttc gcggcgaaaa gatcggtcat cgcaagagcc tcgatcgcgg tgtttccgtg
     1501 gagtttgccg aatacaaaga ctaccacccc ggggatgatc tgcgtttcat cgactggaac
     1561 ctctacggac gtctcgagaa gctctacgtg aaaaagttcc acagcgaaga agatctgcag
     1621 gtgagcatct ttctcgatac ctcggcatcg atggggtttg gctcgccaag caaattcgat
     1681 catgccagac gtctggcagc gggaatcgcg ttcattgcgt ttcagaatca ggatgcggct
     1741 cgtctgcagg tgtttgccga cggcttgaat actgccaccg aaagtggata tcgccctgga
     1801 cacattcatc ggctcatggg gtttctcgag aaacttcagc cgagtggtc
//
LOCUS       DAORHD010001080         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81714_length_1849_cov_3.143813, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001080 DAORHD010000000
VERSION     DAORHD010001080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81714_length_1849_cov_3.143813"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..491
                     /locus_tag="PKO06_18800"
     CDS             33..491
                     /locus_tag="PKO06_18800"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HNV71762.1"
                     /translation="MAYCTREDIEKDLPKADLIQLTDDEGAVTGEDDSDETQVELRVS
                     EAIAKGDAVIDGYLRSRNVTLPLTSVPKLINTLSVDLAIWGLYKRRISQEMPQTIADR
                     YKNAINLLQQIQAGKLGWGDGTTAPAVPAEYRTDMQKRDRLFPKRVLDKF"
     gene            501..941
                     /locus_tag="PKO06_18805"
     CDS             501..941
                     /locus_tag="PKO06_18805"
                     /inference="COORDINATES: protein motif:HMM:NF017736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HNV71763.1"
                     /translation="MIDMRGPYMIAEDWKRVRHFTPDEAWGDRRRVHRNLVFLLDAFR
                     AFIGRPVVIHNAYAQNGHTDESYHYRGMAADLHVEGLNVFDQFIEATRFEFGGIGVYP
                     FWNNPGLHLDIRPRSGAFAPEARWMRTKAGVYVPLTWQNLKEAA"
     gene            938..1303
                     /locus_tag="PKO06_18810"
     CDS             938..1303
                     /locus_tag="PKO06_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71764.1"
                     /translation="MNTATVLILIVLVIQVYKLARPMLKGTETGSKIVNTVDTAWAFV
                     YDFSPAIYTIVEALEKKGVIKKEAKASEFLKLLQEEAKKQGVTLAPANEAAAVLQAKT
                     MAAQDHIAPVPFVQAPPVQ"
     gene            1300..1500
                     /locus_tag="PKO06_18815"
     CDS             1300..1500
                     /locus_tag="PKO06_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71765.1"
                     /translation="MTATVGRDESTIGVALGNALLRYRAARGEKGSGSVSYQDPRTGV
                     IVTGTVANGKPGLQVEIPLLKR"
     gene            1502..>1849
                     /locus_tag="PKO06_18820"
     CDS             1502..>1849
                     /locus_tag="PKO06_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71766.1"
                     /translation="MTSTISQIEDELLEQLGTLDLFRIIDSIGRRDTPPPLNFPAAYV
                     YFLFDRQMQSSPRPVYERRFDLVIAAKNLKSEKEAAQDAYALVEAARDALIGKDLGLT
                     GVGPFECLSINIAL"
BASE COUNT          394 a          540 c          562 g          353 t
ORIGIN      
        1 cgacgacctg cgatcggctg gagtattgat ccgtggccta ctgcactcgg gaagacatcg
       61 aaaaggatct gccgaaagcc gatctgattc agctgacgga cgatgaaggc gcggtcaccg
      121 gcgaggatga cagcgacgaa acgcaggtcg aactgcgtgt ctcggaagcc attgccaagg
      181 gagacgccgt catcgacggg tatttacgct ctcgcaacgt gactctgccg ctgacatccg
      241 tgcccaagct gatcaacacg ctgtcggtcg atctggcgat ctgggggctc tacaagcgcc
      301 ggatcagcca ggagatgccg cagacgatcg ccgaccgtta caagaacgcg atcaatctgc
      361 tgcagcagat tcaggccgga aaactcggct ggggggacgg gacgaccgcc cccgccgtgc
      421 cggcggaata tcgcaccgac atgcaaaaac gcgatcgact cttcccgaag cgcgttctgg
      481 acaagtttta ggaggttcac atgatcgaca tgcgtggccc atacatgatc gcggaagact
      541 ggaagcgcgt tcgccacttc acgccggatg aagcctgggg tgacaggcgg agggtgcatc
      601 gtaacctggt gtttcttctc gatgcatttc gcgcgttcat cggtcggccc gtggtgattc
      661 acaacgcgta tgcccagaac gggcataccg acgagtcgta tcactatcgc ggcatggctg
      721 ctgatctgca tgtggaaggg ttgaacgtgt tcgatcagtt catcgaagca acccgcttcg
      781 agttcggtgg aatcggggtc tatccgttct ggaacaaccc cggactgcat ctcgacattc
      841 gtccgcgttc gggggcattc gcccccgaag cgcggtggat gcgcaccaag gcaggcgtct
      901 atgtgcccct gacctggcaa aatctcaagg aggccgcatg aatacggcaa cggttctgat
      961 tctcatcgtt cttgtcattc aggtctacaa actcgcccgt ccgatgctga aaggcacgga
     1021 gaccggttcg aagatcgtca acaccgtcga taccgcctgg gcgttcgtct acgacttctc
     1081 tcccgcgatc tacacgatcg tcgaagcgct cgaaaagaag ggcgtgatca agaaagaagc
     1141 caaggcgagc gagttcctga aacttctgca ggaagaggcg aaaaagcagg gcgttacgct
     1201 cgctccggcc aatgaggcgg cggcagtgct gcaagccaag accatggccg cgcaggatca
     1261 cattgctccc gtccctttcg tgcaagcccc gccggttcag tgacggccac ggtggggcgt
     1321 gatgaaagca cgatcggcgt cgctctgggc aatgcgctgc tgcggtatcg ggccgctcgc
     1381 ggcgagaaag gttcgggcag cgtctcttac caggacccgc gtaccggcgt catcgtcacg
     1441 ggcaccgtgg cgaacggcaa acccgggttg caggtcgaga ttcccctgct gaagcgctga
     1501 catgacttcc accatcagcc agatcgaaga cgagctgctt gaacagctcg gaacgctcga
     1561 tctgttcagg atcatcgatt cgatcgggcg acgggatacg ccccctccgc tcaacttccc
     1621 ggcagcctat gtctactttc tcttcgatcg gcagatgcaa tcgagcccgc gcccggtcta
     1681 cgaacgccgg tttgatctgg tgatcgcggc aaagaatctg aaaagtgaaa aggaagccgc
     1741 tcaggatgcg tatgcgctgg tggaagcggc ccgcgatgcg ctcatcggca aggatctcgg
     1801 gctgaccggc gtcggcccgt tcgagtgtct gagtatcaac atcgctctt
//
LOCUS       DAORHD010001081         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_81717_length_1849_cov_3.076923, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001081 DAORHD010000000
VERSION     DAORHD010001081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81717_length_1849_cov_3.076923"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1227
                     /locus_tag="PKO06_18825"
                     /pseudo
     CDS             1..1227
                     /locus_tag="PKO06_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
     gene            1302..1817
                     /locus_tag="PKO06_18830"
     CDS             1302..1817
                     /locus_tag="PKO06_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71767.1"
                     /translation="MDSLARVWRSRWRWCALLLCCFVACAVAASASREQARPKACAAN
                     MRVLAGAIEMYNMDHSAPMRDVNPTTLAELVKASYLKCPLTPPSPGCSYSGRNLDKTT
                     DMTQEIRCAEHGTVAELNVYFERLQHTQKMKQIIVVGTVAMALLIILGTIFWGSTSRR
                     KPDEQTDGNAA"
BASE COUNT          411 a          540 c          573 g          325 t
ORIGIN      
        1 gtcgaccatt acgcccgacg agggccgtaa cgtgccgtgg cactacaaca acattcgcgg
       61 catcgaaacc gccaagcaga cgctgaccgc cctcgacggt atcaagcatc tgatcaagct
      121 cgatcgcgac ggtccgctcg gcgaaatggt gcgcgaactg aaggaactgt cgctctgctt
      181 cctggaagaa ctgatcgaag ccggtggcta cttcgccgcc gtcgagcagg gcttcttcgt
      241 cgattccgcc aaatacccgc agcgcaacgg cgacggcatc gcgcgcgatg gcaagggtgg
      301 ggtgggggcc ggcacgttcg tgaaacgcgc cgccgactac ctcgcacccg tctgctcgca
      361 cttcggaaac aacaacctgt cgaaaggcgt taaaaaaccg tgtgagccga tcggagcttg
      421 tactctgtgc gacggttcga aaatcgtctt catcaacgag ctcgacgaga atgattccgc
      481 cgacaaacgc ctcgggcgac cctgccgttc cgcaagggca acgagatcaa gcccgaagtg
      541 gaatgggccg gcgacggcac cgtgttgatc cagctcttcc tgcccgaaga gatcgaactg
      601 gcgcgcgtgg cggccatgga agtcgcgaag aagatgggtt tgaacgaccc tgaagtgatc
      661 aacgctcagg tcatgcaccc gtccgagggc accttcctcg aagtgaaggg caaggtcggc
      721 ttctcgatcg atcggcgcaa tttgaaagtg ccgccgaaag aagtgatcct gcccgaagaa
      781 gagattcgcg ccgagatcaa gaagatgggg ctcaaggtcg tggccgcgac cgtcggcgaa
      841 gacgagcatt ccgtcggtct gcgcgaaatt ctcgacatca aacatggcgg tatcgagaag
      901 tacggcgtga agtatcacta cctcggcacg tcggtgccgc tgggcaagct gatcgacgcg
      961 gccgtcgaga ccggcgcgca ggcgattctg atcagcacca tcatctcgca caacgacgtg
     1021 catcgcacca acatgcgcaa gctcgacgag ctctgccgcg agaagggcct gcgcgacaag
     1081 ctgctgctca tctcgggcgg cacgcaggtc acccgtgaga tcgcgcgcga ggccggcatg
     1141 gatgacggct tcggccgcgg caccaaggga attcacgtct gcaactcgat ggtgaagatt
     1201 gcccgtgcca aaggctggct gaagtaaagg ttgagtgcca ttcagtgccc gatggttcgg
     1261 gcactgaatg gcgagatcat tgattgagag ctggtacgac tatggattca ctcgcgcggg
     1321 tgtggcgaag tcggtggcga tggtgcgcgc tgttgctgtg ctgtttcgtg gcttgcgcag
     1381 tcgctgcatc ggcctcgcgt gagcaggcgc gtccaaaggc gtgtgccgcc aacatgcggg
     1441 ttctggccgg agccatcgag atgtacaaca tggaccattc ggcaccgatg cgcgatgtca
     1501 atccgacgac tctggcagaa ctggtcaaag catcgtatct gaagtgcccg ctgacgcccc
     1561 catcgcccgg gtgctcgtat tcgggccgaa acctcgataa gacaacggac atgacgcagg
     1621 agatccggtg cgcggaacat ggaaccgtgg ccgaactcaa tgtctatttc gaacggctgc
     1681 agcataccca gaaaatgaag cagatcatcg tcgtcggcac ggtagccatg gcactgctca
     1741 tcattctcgg cacgatcttc tggggaagca cttctcgccg caaaccggat gaacagaccg
     1801 acgggaacgc cgcgtgagcg ccacaaagca gatgcagaag ctgtttacc
//
LOCUS       DAORHD010001082         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82053_length_1845_cov_2.720112, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001082 DAORHD010000000
VERSION     DAORHD010001082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82053_length_1845_cov_2.720112"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PKO06_18835"
     CDS             <1..940
                     /locus_tag="PKO06_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71768.1"
                     /translation="ADVVSDCEYPLDESVGAELSADCRTRSPELGNLISDRRTFREFA
                     RARGLLPLEFWTVETFEQLSAVILKRERFPLCLKSRRNLSGGVGTYRIEGFRELSRFW
                     ERLQKVAPGPAIIEDWVNAQVLLEVTLVPDGSMLAVQAGLEQSLTCRTAWRMFPVVIP
                     ERWKAGMEKIVAAVKSVGDGWNQPLRLHLGLMPDGQTIPLALNGGLNRPEYLPRWSGG
                     GWGGRALVSRWLGALTTAPLSKGQVVRLLQLRRPSRLEAFPDRLPDSVRSQPSVRDYA
                     AVGRHALVMLIGADPKKLAAEGRAVATLLAAQEDSD"
     gene            complement(968..>1845)
                     /locus_tag="PKO06_18840"
     CDS             complement(968..>1845)
                     /locus_tag="PKO06_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71769.1"
                     /translation="STAAAAPAAGTAAGTTAIPDPKQGIEYVRVKSGDSLSKICQNYY
                     GNSGMWVHIVKYQIPTIAANPNLIFPGQLIALPRRLGTSEPADHSTGPYTPPTTSSGS
                     TPPTASPGDESWQERFQKDYVVSDHTLTDSKSMTVAQIQAFLDKKGSCLAKPYRGSTP
                     AQMIYDACQKHGISPKVILTRLQCEQGMISKKTASQKDLDWALGVGCYDSGNWNQSFK
                     GFDKQIEGAAQTYRRHYDAAKAKLDRGEAVTMTIDGETVRIKNAATYAFYKYCPHFHG
                     NKLFYDVWNGYKKVW"
BASE COUNT          338 a          487 c          581 g          439 t
ORIGIN      
        1 tgcggacgtc gtctccgatt gtgagtatcc tctcgatgag tcagtaggag cagaattgtc
       61 tgccgactgc cgcacacgtt caccagaact gggcaatctg atatccgatc gccgcacttt
      121 tcgagaattc gcacgcgcgc gcggtttgtt gcccttggag ttctggacgg tcgagacgtt
      181 tgagcaactg agtgccgtca tactcaagcg cgaacggttc ccgctgtgcc tgaaaagcag
      241 acgtaatctg tctggtgggg ttggaaccta tcgtatcgaa ggtttccgtg aactgtcgcg
      301 tttctgggag cggctgcaga aagtggcacc tgggccggct atcatcgaag actgggtgaa
      361 tgcgcaggtt ctactggaag tcactctggt tcctgatggc tcgatgctgg cggtgcaggc
      421 ggggctcgag cagtcgctca cgtgccgcac ggcctggaga atgtttccgg tggtgattcc
      481 tgaacggtgg aaagcgggga tggagaagat cgtcgccgca gtgaaatcgg ttggagacgg
      541 ctggaatcaa ccactcagac tgcatctggg cctgatgccg gatggacaaa cgataccact
      601 ggcattgaat ggaggcctga accgcccgga atatctgcct cgctggtcag ggggtggctg
      661 gggtgggcgt gcactcgttt cccgctggtt gggagctctg acgacggcac cattgtcgaa
      721 ggggcaggtg gtgagattgc tgcagcttcg ccgcccaagt cgattggaag cgtttcccga
      781 taggctccct gattccgtgc gctcgcagcc ttccgttcgc gattatgctg cggtcggtcg
      841 tcatgcactg gtgatgttga ttggcgctga ccccaagaag ctggccgccg aaggacgcgc
      901 ggtggcgaca cttctggcgg cccaggaaga cagcgactga cgaaggtcac cggttcagaa
      961 ccgggtttca ccacaccttt ttgtatccat tccagacatc gtagaacagt ttgttgccat
     1021 ggaaatgggg gcagtacttg taaaacgcgt aggttgccgc gttcttgatg cgcacggttt
     1081 cgccatcaat cgtcatcgtt accgcttcac cgcggtccag cttggctttg gcggcatcat
     1141 aatggcggcg ataggtttgt gccgcgcctt cgatctgctt gtcgaagccc ttgaagctct
     1201 ggttccagtt gcccgaatcg tagcagccaa cgcccagcgc ccaatcgaga tctttctgtg
     1261 aggcggtctt cttcgagatc atgccctgtt cgcactgcaa ccgggtgaga attactttcg
     1321 gactgatgcc gtgtttctga caggcatcgt aaatcatctg agctggtgtt gagccgcgat
     1381 agggcttggc cagacacgaa cctttcttgt cgaggaaagc ctggatctgc gccactgtca
     1441 tcgacttcga atcggtcaga gtgtgatcag agacgacata gtctttctgg aaacgttcct
     1501 gccacgattc gtctccgggc gaggctgtcg ggggcgtgct gccgctgctg gttgtcggcg
     1561 gagtatatgg tcctgtggaa tggtctgccg gctcactcgt tccaaggcgg cgcggcaacg
     1621 cgatcagttg tccgggaaag atcaggttcg gattcgctgc gatcgtggga atctggtatt
     1681 tcacgatatg cacccacatc cccgagttgc cgtagtagtt ctggcagatt ttcgacagac
     1741 tgtcacccga tttgacacgc acgtattcga tcccctgctt gggatcgggt attgctgttg
     1801 tgccggcggc ggttccggcg gccggagcgg ctgccgccgt agagg
//
LOCUS       DAORHD010001083         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82281_length_1842_cov_1.982093, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001083 DAORHD010000000
VERSION     DAORHD010001083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82281_length_1842_cov_1.982093"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKO06_18845"
     CDS             <1..255
                     /locus_tag="PKO06_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71770.1"
                     /translation="GIPLRWYRIGVAFDYQHDDLVASHCWLLLYNMSAASQPVWFDGV
                     QLERAVVPGQTRPTAYAPHKKIVSPGRSIDLPGKKHYYEW"
     gene            245..847
                     /locus_tag="PKO06_18850"
     CDS             245..847
                     /locus_tag="PKO06_18850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71771.1"
                     /translation="MSGNRIKEQKRWANRLGTRGSAMLVMLVAVMVAGIIGSQLATRT
                     EVRTTRDREEQLRHTLARIRNAFDLKFRLDAAYDPDLSTPALIQQSLQQLVQDRLLGR
                     EELRDPLVTPSLWGMHAEHYWKSVGNVSVNSSFESDAAPDLTKSEVVASWSAGDINTT
                     AAPDADFTPSRFSGEFDDYLQQNKLGRPMTASGTSLRVDN"
     gene            complement(864..1736)
                     /locus_tag="PKO06_18855"
     CDS             complement(864..1736)
                     /locus_tag="PKO06_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71772.1"
                     /translation="MRVGFYLLSAGRLDLERSDLPLDFYLWLIHTGSDTPQFEFLNSR
                     DARVVRQNTQEAGMPGLPGAQTTVFRVTGHFEQDLDMSRYPFENHEIKIAFEDSEADL
                     TRRRYLIDGTGITRDAGFQIVGWNVDSCKVETATHSYSSNFGVVGAPLQEYAQVLVSM
                     QISRPRGLMFLKTFVPVLLFVTIAMMGLFFPVDNLNQKVGLAVGGLFSSVAYHVNLTR
                     NMPPVGYLTVVDRLLIGQYALLFLTLLTAIITFIACKAEFEREARLISTLGRLLIPTA
                     AVVLYLLQLSDILF"
BASE COUNT          426 a          480 c          558 g          378 t
ORIGIN      
        1 ggaattccgc tgcgttggta ccggattggg gttgcgttcg actatcagca cgatgacctg
       61 gtggcgtcgc attgctggct tctgctgtac aatatgtccg cggcgtcgca gccggtgtgg
      121 ttcgatggag tgcagctcga acgcgcggtc gttccgggtc agacaagacc gacggcctat
      181 gcgccgcata agaagattgt cagtccgggc cggtcaatcg atctaccggg aaagaagcat
      241 tactatgagt ggtaatcgaa tcaaggaaca gaagcgttgg gcaaaccgtc tgggcacacg
      301 gggaagtgcg atgctggtga tgcttgtcgc agtgatggtc gccgggatca ttggctcgca
      361 gctggcgacc agaaccgaag tgcgcactac gcgtgatcgc gaagaacaac tgcggcatac
      421 cctggctcgg atccgtaacg cattcgatct gaagtttcgt ctcgatgccg cgtatgaccc
      481 cgatctttcg accccggctc tgatccagca gagtctgcag cagttggtcc aggaccgatt
      541 gctgggacgc gaagaactcc gtgatccgct tgtcacgcct tcattgtggg gtatgcacgc
      601 cgagcactat tggaaaagcg tcgggaacgt ttccgtgaat tcaagcttcg agtcggatgc
      661 agcgcccgat ctgaccaaga gcgaagtggt cgcttcgtgg tcggcggggg acatcaacac
      721 gactgctgct cctgatgccg acttcactcc ttcccgtttc agcggagagt ttgacgacta
      781 tttgcagcag aacaagctgg ggcggccgat gaccgcatcc ggaacctcat tgcgcgttga
      841 caactgaacg tttgtcaggc gcttcagaag agaatatctg agagttgcag caggtacaaa
      901 acgaccgcag ccgtcgggat caggagtcgg ccgagggtgg agatcagacg agcctctcgc
      961 tcgaactctg ctttgcaagc aataaacgtg atgatagctg tcagcagggt gagaaacagc
     1021 agcgcatact gaccgatgag caggcgatca acaacggtga gatacccgac ggggggcatg
     1081 tttcgggtca agttgacgtg ataagcaacc gaactgaaga ggccaccgac cgccagaccg
     1141 actttctgat tcagattgtc gaccggaaaa aagagcccca tcatggcgat ggttacgaac
     1201 agcagcacgg gaacgaaggt ttttaggaac atcagaccgc gtggtcgtga gatctgcatg
     1261 gaaaccagaa cctgagcata ctcctgcaga ggagcgccca cgacgccgaa gttggatgag
     1321 tatgagtgtg ttgctgtctc aactttgcag gaatcgacgt tccaaccgac aatttgaaaa
     1381 ccggcatcac gcgtgattcc ggttccgtcg atcaggtagc gtctccgggt cagatctgct
     1441 tcagaatctt caaatgcgat tttgatttcg tggttctcga acggatatcg actcatatcg
     1501 agatcctgct cgaagtgccc ggtcacccga aagactgtcg tctgagcgcc cggcagccct
     1561 ggcatgccgg cttcctgagt gttctgccgg acgacccggg catcgcgact gttgagaaac
     1621 tcgaactggg gtgtgtcaga gccggtgtga atcaaccaga gatagaagtc gagcgggaga
     1681 tctgaacgct cgagatcgag ccgaccggct gacagcagat agaatccgac ccgcacggtg
     1741 tctgcatctg aatctggcgt tgtcgccaat acctgggccc aggatgtggc catgcagagc
     1801 atgagaccca ggtattggcg acaacgccag attcagatgc ag
//
LOCUS       DAORHD010001084         1840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82409_length_1840_cov_2.672829, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001084 DAORHD010000000
VERSION     DAORHD010001084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82409_length_1840_cov_2.672829"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKO06_18860"
     CDS             complement(<1..311)
                     /locus_tag="PKO06_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNV71773.1"
                     /translation="MSTYQYVGKSVERVDSLEKVTGAAQYVDDINFGPDLLHAEIVES
                     PHAHALIKSIDTSAAEKVPGVVKVVTGKDFPVKFGMYMKDRYIFAIDRVRFIGEQVAA
                     VV"
     gene            complement(314..790)
                     /locus_tag="PKO06_18865"
     CDS             complement(314..790)
                     /locus_tag="PKO06_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV71774.1"
                     /translation="MKTHAISFTLNGEPWTLEVEPSDTLLEAIRDKMGIKSPKVACDR
                     GDCGACTVLLDGKAVRSCLILAVEIDGHEVVTVEGLSKQSPTPLQQSFAHNNAFQCGF
                     CAPGMVLAAHELLQRNPHPTREEAQEAISGNLCRCTGYEKILECITDPHLKLPEDK"
     gene            complement(800..1690)
                     /locus_tag="PKO06_18870"
     CDS             complement(800..1690)
                     /locus_tag="PKO06_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HNV71775.1"
                     /translation="MTIAHPFEYHRPTTLADAVRLLADYQGKASVLAGGTDLINWLKE
                     DFVTPPAVVDIKAIADLKSIGWVNNTLAIGATTTFSELMHSPQVQELFPLIVEQSHVV
                     ACHGVRNRATMVGNICSGVPCCDSGPILLVYDATVQVVGPKGARAIPISQWFVGPKKT
                     SRDPQEIVTRVDVPLPAKKSAGVYVKLGRYRGEDLAQASVAMLAMEDGEIRVAFGSVA
                     PTPIRSRKIEDLLRGQVVTDALLQKLDTLVEAEIAPISDLRATKEYRLHTTKIMTRRA
                     VQAVHQRLAGRGPAYGTSLL"
BASE COUNT          355 a          554 c          549 g          382 t
ORIGIN      
        1 accacggccg ccacctgttc tccgatgaaa cgaacccggt caatggcgaa aatgtagcgg
       61 tccttcatat acatgccgaa cttgaccgga aagtccttgc cggttacgac cttgaccacg
      121 ccgggcacct tctcggccgc cgaggtgtcg atcgatttga tcagcgcgtg cgcatgcgga
      181 ctctcgacga tctccgcgtg cagcagatcg gggccgaaat tgatgtcgtc gacgtactgg
      241 gccgctcccg tgactttctc gaggctgtcg acccgttcga ccgattttcc gacatactga
      301 taggttgaca tgattacttg tcctccggca gcttcaaatg ggggtcggtg atgcactcga
      361 gaatcttctc ataccccgtg caacggcaca ggttcccgga aatggcttcc tgagcctctt
      421 cgcgggtcgg gtgcggattg cgctgcagca gttcgtgggc cgcgagtacc atgccgggag
      481 cgcagaaacc gcattgaaat gcgttgttat gagcaaacga ttgctgcagc ggggtggggg
      541 actgcttcga aagcccttca accgtcacga cctcgtggcc atcgatctcg accgccagaa
      601 tcagacagct gcgaaccgcc ttgccgtcga gcagcacggt gcaggcaccg cagtcgccgc
      661 ggtcacaggc gactttgggg ctcttgatgc ccatcttgtc gcgaatcgct tcgagcaggg
      721 tatcgctggg ctcgacctcg agggtccagg gttctccgtt cagggtgaag gaaatggcat
      781 gcgttttcat gggttctcct tacaacaggc tggtcccata agcggggcca cggccggcga
      841 gacgctgatg cactgcctgc actgcgcgtc gggtcatgat cttcgtcgtg tgcaaccggt
      901 actctttggt cgcacgaaga tcgctgatcg gagcgatctc cgcttccacc agggtgtcga
      961 gtttctgtag cagagcgtcg gtcaccacct gtccgcgcag cagatcctcg atcttgcgac
     1021 tgcgaatcgg tgtcggagcg accgatccga atgcgacccg gatctctccg tcttccatgg
     1081 ccagcatggc cacgctggcc tgtgccagat cctcgccccg atagcggccg agtttcacgt
     1141 aaacgcccgc gctctttttc gctggtagcg gcacgtcgac tcgcgtcacg atttcctgcg
     1201 ggtcgcgtga ggttttcttc ggcccgacga accactgcga aatcgggatc gcccgagcac
     1261 ccttcggccc cacaacctgg acagttgcat catagaccag cagaatcggc ccgctgtcac
     1321 agcacggcac gcctgaacag atgttgccga ccatggtggc gcggttgcgt acgccgtgac
     1381 aggccaccac atgactctgc tcgacgatca gcggaaagag ttcctgtact tgcggcgagt
     1441 gcatcaactc gctgaaggtc gtcgtggcgc cgatggcgag cgtgttgtta acccagccga
     1501 tcgacttcag gtcggcaatg gccttgatgt cgacgaccgc cgggggagtg acgaaatctt
     1561 ccttgagcca gttgatcagg tccgtgccac cagccaggac gctcgctttg ccctggtagt
     1621 ccgcgagaag ccgcacggca tccgccagcg tggtcgggcg gtgatactcg aacgggtgtg
     1681 cgatggtcat gattcctcca gaaacgacaa tgatcgttgc gaaagccgca acaggacgga
     1741 aaacctctcc ttgtgcatct cgtatgccgt cttctgctgg aaaaaaaagt gttcggcagg
     1801 cgatgaacgg acatcgaagt gtagcgcacg aaagaaacgc
//
LOCUS       DAORHD010001085         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82603_length_1837_cov_3.888889, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001085 DAORHD010000000
VERSION     DAORHD010001085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82603_length_1837_cov_3.888889"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKO06_18875"
     CDS             <1..729
                     /locus_tag="PKO06_18875"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNV71776.1"
                     /translation="PDGTRRVPSTAILLTVPPSTKGQPALLTHGDLEMLLHEFGHAFH
                     HLFSQATYARLAGTGVSLDFAEVPSTLLEELAWDPTVLKSISRHHPSGRSLSDDEIGR
                     LIRGRPNQSPLAFQNRLFKCLFDLHLHTGSITNTTDLYSRLQEQLFLIPMTPGTAPHA
                     AFAHLMGGYDASTYAYPLATVLTADLLSAFSRKGLTIPAPWRRFRQVVLAPGRVPDEE
                     ALLNTFLGRPWSVNSFLQRFTTRH"
     gene            940..1518
                     /locus_tag="PKO06_18880"
     CDS             940..1518
                     /locus_tag="PKO06_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71777.1"
                     /translation="MFVLIPVLFVVFFAVSGYAQDTTEASDASFQGLITGVPGPHQVE
                     ASSEVGGQVDAEPGPRKMPLPRGFDFWQKERDAKAIRGWEYAFDYSGEFLHVSKNHDG
                     KTKQVYLDNLNLKLGVDLEKAQLGAKGRFFLYVLGNSGGNITGDHLDASGIGRVGDLQ
                     TLSNIETNETFRLYEAVLRVAGREVDDPGRNS"
     gene            1496..>1837
                     /locus_tag="PKO06_18885"
     CDS             1496..>1837
                     /locus_tag="PKO06_18885"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HNV71778.1"
                     /translation="MILAGIHDLNADFDNSEYAAFFSNSSFGIAPDISKNAPVSIFNV
                     TSPALRLKYEPNAHWTFLAGIWDGYPGTPDENERGFGLRITPREGYMNIVEVQHHHHL
                     FGSHLPGTIKVG"
BASE COUNT          370 a          563 c          478 g          426 t
ORIGIN      
        1 ccggatggga ctcggcgcgt tccttcgaca gccatcctcc tgactgttcc accatcgacc
       61 aaagggcaac cggctctgct cacgcacggc gacctcgaga tgcttctgca tgagttcggt
      121 cacgcctttc accacctgtt ctcgcaggcg acgtatgcgc gactggcggg aaccggcgtc
      181 tcgctcgact ttgccgaagt tccttcaact ctcctggaag agctcgcctg ggatccgacg
      241 gttctgaaga gtatcagtcg acaccatccg agcggtcggt cgctctcgga cgatgaaatt
      301 ggccgcctga tccgcgggcg gccgaaccag tcacccctcg ctttccagaa tcgacttttc
      361 aagtgtctgt tcgatctaca tcttcacact ggctcgatca ccaacaccac cgacttgtac
      421 tctcgacttc aagagcagct gttcctgatc cccatgaccc cgggaacagc ccctcatgcc
      481 gcatttgcac atctgatggg cgggtatgat gcgtccacct acgcatatcc cctggccacc
      541 gttctgacag cggatctttt gtctgccttc agccgaaaag ggctcacgat tcccgctccc
      601 tggagacgtt ttcgccaggt cgtgctcgct cccggtcgcg tccccgacga ggaagcgctt
      661 ctcaacacgt ttctgggtcg tccgtggagc gtcaactcct tcctgcagcg cttcaccacg
      721 cgccactgac ggacctcatc cgcgcgggga tttcatcgcg gattccgtcg ttctcgttcg
      781 tccacccgat ttcgatcatc gcatcgatct cagttcccgc cattcctgag gaattggtgg
      841 cgagcgaaca cgacgggcgt atggaaattc ttcagagaaa gttgcacgtt tcaggtattc
      901 tgatacactt ttcctcatat gaaatctcta cgaggatcct tgttcgttct cattccagtt
      961 cttttcgtgg ttttcttcgc ggtttccggc tatgcccagg atacgacaga agcgtcagat
     1021 gctagttttc aaggcctgat cactggggta cctggccctc accaggtgga agccagcagc
     1081 gaagtgggcg gtcaggttga tgcagagccc gggccgcgga aaatgcctct tccccgtggg
     1141 tttgacttct ggcagaaaga gcgcgacgcg aaagccatac gcggctggga gtatgcgttc
     1201 gactactcag gcgagtttct gcatgtttcc aagaaccatg acggaaaaac caagcaggtc
     1261 tatctcgaca atctcaatct gaaactcggt gtcgatctcg agaaagccca gttgggcgcc
     1321 aagggccgct tcttcctgta cgtgctcggg aacagcggag gcaacatcac cggcgatcac
     1381 cttgacgcat ctggcatcgg cagggtcggc gatctgcaga cgctgtcgaa cattgagacc
     1441 aatgagactt ttcggctgta tgaagccgta cttcgagtgg ccggtcggga agtggatgat
     1501 cctggccgga attcatgatc tcaatgcgga tttcgacaac agcgagtacg ccgccttctt
     1561 ctcgaacagc tctttcggta ttgctccgga catttccaag aatgccccgg tcagtatctt
     1621 caacgtcacc tctcccgcac tacgactcaa atatgaaccg aatgcccact ggacgtttct
     1681 cgccggcatc tgggacggat atcccggcac gcccgatgaa aacgagcgcg ggtttggtct
     1741 ccgcatcact ccgcgagaag ggtacatgaa catcgtcgaa gttcagcatc atcatcacct
     1801 gttcggcagt caccttcccg gaactatcaa agtcggg
//
LOCUS       DAORHD010001086         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82612_length_1837_cov_3.170595, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001086 DAORHD010000000
VERSION     DAORHD010001086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82612_length_1837_cov_3.170595"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /gene="gltA"
                     /locus_tag="PKO06_18890"
     CDS             complement(<1..1075)
                     /gene="gltA"
                     /locus_tag="PKO06_18890"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNV71779.1"
                     /translation="MAKKIIEKKHPMPHLDPKERIRGFEEVATGYTRETAVAEAERCL
                     QCKNPFCVQGCPVEVPIPEFIGMLAKGDFDGAICKIKEKNALPAICGRVCPQESQCEK
                     LCVLGKKFQPVAIGRLERFVADWEIENKKQEDAPTAHPTGAKVAVVGAGPAGLTCAAD
                     LARYGYQVTVFEALHEAGGVLVYGIPQFRLPKEIVRREVDNIRKLGVDFQFNMVIGRI
                     HTVDELFAEGYKAVFLGTGAGLPYFMNVPGEHYNGVYSANEWLTRTNLMRAYKFPEYR
                     TPIKFGKKVAVVGGGNVAMDAVRTALRLGAEKAYIIYRRTENEMPARLEEIEHAHEEG
                     IEFLFLTNPVEIKGDEKGNVRAVV"
     gene            complement(1079..>1837)
                     /locus_tag="PKO06_18895"
     CDS             complement(1079..>1837)
                     /locus_tag="PKO06_18895"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNV71780.1"
                     /translation="GQFVIVRKDEKAERIPLTIADYDRTNGTITLIVQEVGKSTKEMG
                     ELKAGDAFLDFVGPLGMPSELHKFGTVVTVGGGLGIAPVYPIAKELKKMGNRMINIIG
                     ARNKDMLFWVDEMKAISDDLLIATDDGSLGEKGFVTTLLDKVIKREKVDFVMAIGPMV
                     MMRACCEVTRGPAVPTKVSLNPVMVDGTGMCGGCRVLVDKKVKFACVDGPEFDGHLVD
                     WQAAIARSKMFWDLEQKAHAEDNHECRLNKATGA"
BASE COUNT          318 a          550 c          550 g          419 t
ORIGIN      
        1 gcacgacggc gcgcacgttg cccttctcgt cgcccttgat ctcgaccggg ttggtcagaa
       61 acaggaactc gatgccctct tcgtgggcgt gctcgatctc ttcgagtcgg gcgggcattt
      121 cgttctcggt gcggcgatag atgatatacg ccttttcggc gccgaggcgc agtgcggtgc
      181 gcacggcatc catggcgaca ttgccgccac cgaccacggc gaccttcttt ccaaatttga
      241 tcggggttcg gtattcaggg aacttgtacg ctcgcatcag gttggtgcgg gtcagccact
      301 cgttggccga gtagacgccg ttgtaatgct cgcccggcac attcatgaag tagggcaggc
      361 cggcgccggt gccgagaaac acggctttgt acccctcggc gaacagttcg tcgacggtgt
      421 gaatgcggcc gatgaccatg ttgaactgaa aatcgacccc gagtttgcga atgttatcga
      481 cttcgcggcg cacgatttct ttgggcagtc ggaactgcgg aatgccgtag accagcacgc
      541 cgcctgcttc gtgcagggcc tcgaacaccg tcacctggta gccatagcgg gcaagatcgg
      601 cggcacaggt cagaccggcc gggccggcac caaccaccgc gaccttcgct ccggtcgggt
      661 gagcggtggg agcgtcttcc tgtttcttgt tctcgatctc ccagtcggcg acgaaccgtt
      721 cgagacggcc gatcgcgacg ggttgaaact tcttgccgag cacgcagagt ttttcgcact
      781 ggctttcctg cgggcaaacg cgaccgcaga tcgcgggcaa ggcattcttc tctttgattt
      841 tgcagatcgc gccgtcgaaa tcgcccttgg caagcatccc gatgaattcc ggaatgggta
      901 cttcgaccgg gcacccctgt acgcagaacg gattcttgca ctggagacag cgctcggctt
      961 cggcgacggc ggtttcgcgg gtgtaaccgg tggcgacctc ctcgaagccg cgaatgcgct
     1021 ccttggggtc gaggtggggc atgggatgtt tcttttcgat gatcttcttg gccatgactt
     1081 atgctcctgt cgccttgttc agccggcatt cgtgattatc ttcggcgtgc gctttctgtt
     1141 cgagatccca gaacatcttt gagcgggcga tggccgcctg ccagtcgacc aggtggccgt
     1201 cgaactcggg gccgtcgacg caggcgaact tgaccttctt gtcgaccagc acgcgacaac
     1261 cgccgcacat gccggttccg tcgaccatga ccgggttcag actgaccttg gttggtactg
     1321 ccggtccacg ggtgacttcg caacaggcgc gcatcatcac catcggaccg atcgccatga
     1381 cgaaatcgac tttttcccgt ttgatcactt tgtccaacag ggtcgtgacg aaacccttct
     1441 cgccaagaga gccgtcatcg gtcgcgatca gcagatcgtc gctgatggct ttcatctcat
     1501 cgacccagaa cagcatgtcc ttgttgcggg ccccgatgat gttgatcatg cggtttccca
     1561 tcttcttgag ctctttagcg atcgggtaga cgggcgcgat gccgagccct ccgccgacgg
     1621 tgacaacggt tccgaatttg tgcagttcag agggcatgcc cagcggcccc acgaaatcga
     1681 gaaaggcgtc gccggccttg agttcgccca tttccttcgt tgatttcccg acttcctgca
     1741 cgatcagggt gatcgtgccg ttcgtgcggt cataatcggc gatggtgagc ggaatgcgct
     1801 cggctttctc gtctttgcgt acgatgacga actggcc
//
LOCUS       DAORHD010001087         1836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82709_length_1836_cov_2.503650, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001087 DAORHD010000000
VERSION     DAORHD010001087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82709_length_1836_cov_2.503650"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PKO06_18900"
     CDS             <1..700
                     /locus_tag="PKO06_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009417427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNV71781.1"
                     /translation="EMAFYDIKDNMDLAEAFLKYLTSWALKHCRDDLEFLNKLFLEEE
                     KTRKQEERSEMNLIEKLQFIVDNPFHRMSYTEAFEILRDSNPNKKKKFAYLVDKWGID
                     LQSEHERYLVEKHFKKPVILTDYPKEIKAFYMKLNDDGKTVRGMDVLVPGIGEVIGGS
                     QREERFDVLKQKIAEFKIPEESMWWYLESRKFGTAVHSGFGMGFERFLMFVTGMTNIR
                     DVIAFPRTPKSAEF"
     gene            736..969
                     /locus_tag="PKO06_18905"
     CDS             736..969
                     /locus_tag="PKO06_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71782.1"
                     /translation="MKGSTIAISVLLICVLSYFLFPFAFIIPLVLVFGKSPPEQVRQA
                     FMVFIAPVKYLNDKFEPYRQYLQLQERLLNRFL"
     gene            1048..1491
                     /locus_tag="PKO06_18910"
     CDS             1048..1491
                     /locus_tag="PKO06_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71783.1"
                     /translation="MPTHISFDTLLTRVQRAFALTHDLVASLSDQDLGKKIGDLPSNT
                     IRQQLWCIVGARESYAKAIDNGGWAGFSCSLTQTDARDAILQALKKSELDFVHIVSKS
                     ELTGMQIEYFLALHEHEIQHHGQLIRYVYANRLDFPQSWNKRYTV"
     gene            1624..>1836
                     /locus_tag="PKO06_18915"
     CDS             1624..>1836
                     /locus_tag="PKO06_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71784.1"
                     /translation="MVSSEQKLTLLERYAHANSLLRSFNPFGFDAGDQVMKPGGIAVV
                     FLIICVVCYFAFPIAFVIPLMLVFGRV"
BASE COUNT          423 a          526 c          470 g          417 t
ORIGIN      
        1 ggagatggcc ttctacgaca tcaaggacaa catggatctc gccgaagcat ttctcaaata
       61 tctcacgagc tgggcactga agcactgccg cgatgatctc gagttcctga acaaactgtt
      121 cctcgaagaa gagaagacac gcaagcagga agaacgcagc gagatgaacc tgatcgagaa
      181 gcttcagttc atcgtcgaca acccgttcca ccgcatgagt tacaccgagg cgttcgagat
      241 tctgcgcgac tcgaacccga acaagaagaa gaagttcgcc tacctggtcg acaagtgggg
      301 catagatctg cagtctgaac atgagcgcta tctggtcgag aaacacttca agaagcccgt
      361 cattctcacc gactatccga aggagatcaa agccttctac atgaagctga acgacgatgg
      421 aaagacggtt cgcggcatgg acgtgctggt acccggcatc ggcgaagtca tcggcggttc
      481 gcagcgcgaa gagcgcttcg acgtgctcaa acagaagatc gccgagttca agattcctga
      541 agaaagcatg tggtggtatc tcgaaagccg caagttcggc acagccgtgc acagcggttt
      601 cggcatgggt ttcgagcgct ttctcatgtt cgtcacgggc atgaccaaca tccgtgatgt
      661 cattgccttt cctcgcacgc cgaagagcgc tgagttctag aagggtcaca ggagaaggcc
      721 gagccggcga gcaccatgaa aggttctacc atcgccatct cggtcctttt gatctgcgtc
      781 ctttcctact ttctctttcc attcgcgttc atcattccat tggttctcgt gttcggaaag
      841 tcgccgcccg agcaagtgcg ccaggcgttc atggttttca tcgctccggt gaagtatctg
      901 aatgacaagt tcgagccgta tcgccagtat ctccagcttc aagagcgttt actgaaccgc
      961 tttctctagc aatctccgaa tcttccgttg tcggcggcaa gcgccccgaa actccgcaga
     1021 cacctttcca gggagggatt cagcaacatg cctacgcaca tctcgtttga caccctgctc
     1081 acccgcgtac agcgagcctt tgctctgact cacgatcttg tcgcgagcct gtcagaccag
     1141 gacctcggca aaaaaatcgg cgatctgcct tcgaatacca tcaggcaaca actgtggtgc
     1201 atcgtcggtg cgcgcgagag ctacgcgaag gcaatcgaca acggcggctg ggctggtttc
     1261 tcctgttctc tcacccagac cgacgcgcgc gatgctatcc tgcaggcgtt gaaaaagtcc
     1321 gagctcgatt tcgtgcacat cgtatccaaa tctgagctta caggaatgca aatcgagtat
     1381 tttctcgcct tacacgagca tgagattcaa catcacggcc agctgattcg atatgtctat
     1441 gcgaaccggc tcgatttccc tcagagttgg aacaaacggt acaccgtctg aaacaagctg
     1501 cggtcctgaa tcacgcgaga aacgcttctg gcgcgcaacg agcatttgtc ccctgatcgt
     1561 cagcgggtta acctcgtgtg cccctgacac tcgcagagcc ggaatgtcgt agcagttggt
     1621 ttcatggtat cttcagaaca gaaactcaca ttgctggagc gatatgcgca tgcaaactct
     1681 cttctgcgct cattcaaccc cttcggattc gacgcggggg accaggtcat gaaacccgga
     1741 ggcatcgccg tcgtcttcct gatcatctgc gtcgtgtgtt acttcgcctt cccgatcgcc
     1801 tttgtcatcc ccctgatgct ggttttcggt cgcgtg
//
LOCUS       DAORHD010001088         1835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82738_length_1835_cov_4.515730, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001088 DAORHD010000000
VERSION     DAORHD010001088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82738_length_1835_cov_4.515730"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..646)
                     /locus_tag="PKO06_18920"
     CDS             complement(80..646)
                     /locus_tag="PKO06_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71785.1"
                     /translation="MLEKTLKVVLGLMLMVLYLQRVVMPGIDDLRRERQQLAALQASV
                     SRFRGGEQRERARMGMQQAMMRDHMTQLGRLIPGFDETRTSLQAKFEAIKTAVPGIWS
                     VKPAPSFTDDAAVVRWPFKVVFDGELPAALKAISLIENSGQLARISNLQFSAQPRGRV
                     RVEADLELLYQRQAAVSLAAAPSTEGRP"
     gene            complement(655..>1835)
                     /locus_tag="PKO06_18925"
     CDS             complement(655..>1835)
                     /locus_tag="PKO06_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71786.1"
                     /translation="EMVFALRPLVGNDPKNREYLVSYVNRIFVNRLQSLFESLGFTLN
                     RVTTGIEALIGAWSRQPKARTERQVCLISLGYTSVNMAILRDGQVTAVRTSLSGALKE
                     VELRLMTSLKLKQNEVDDLLTGVAAQADQHSIEIIQQNQRELLARITPFFAYIRAKEK
                     EQGEQTIYLSMPYLEVTGIKPLLEETFKMPVQVLTAGAKTDGAPAPGLADGAWLAGAL
                     EKGLISLVEGRPPFLRFTMTPRLAMMFMVFFLIAPLAFVRVNRSAIYHQTEALKRENE
                     QFRPIFEQARLNRQQFERLAALGEVAQREMTGQVLLAPIIADVAKSLSGEIRLDKVQM
                     SAANRSLMLNGFSIDTETALRFWDSLQKLTALQDVKISFSETSSQGATGFNVTATLKK
                     "
BASE COUNT          364 a          567 c          556 g          348 t
ORIGIN      
        1 accggagcgc tgaccatcat gccgaatgcg aacagacacg cgaagactgc accaatcacc
       61 agaatcttga gctcacgact catgggcgtc cctccgttga cggggcggca gccagagata
      121 ccgcggcctg tcgctggtac aacagctcga gatcggcttc gacccgcacc cgaccacgcg
      181 gctgcgcgct gaactgcagg ttgctgatgc gagccagctg cccggaattt tcgatcaggg
      241 aaatcgcctt caacgccgcc ggcagctcgc cgtcaaacac gaccttgaag ggccagcgca
      301 ccaccgcggc gtcatcggtg aaactggggg ccggtttcac gctccagatg ccgggaaccg
      361 cggtcttgat cgcctcgaac ttggcctgca gactggtgcg ggtttcgtcg aacccgggaa
      421 tcagacggcc gagctgggtc atgtggtcgc gcatcatcgc ctgctgcatg cccatgcggg
      481 cacgttcgcg ctgctcgccg ccgcgaaaac gactgacgga agcctgcaag gcggcaagct
      541 gttgccgctc gcgacgcagg tcgtcaatgc cgggcatgac cacccgctgc agatagagta
      601 ccatcagcat caggccgaga acgactttga gtgttttttc cagcatggtc gtttctactt
      661 tttcagggtc gccgtgacat tgaagccagt ggcgccctgc gaagaggttt ccgagaagct
      721 gatcttgaca tcctgcagcg cggtcagctt ctgcagggaa tcccagaagc gcagcgccgt
      781 ctcggtatcg atcgaaaaac cgttcaacat caacgagcga ttggccgcgc tcatctgcac
      841 cttgtcgagc ctgatctcgc cgctgagact cttcgcgacg tcggcgatga tgggcgccag
      901 cagcacctgt ccggtcattt cgcgctgcgc gacctcgccg agagccgcca gacgctcgaa
      961 ctgctgccgg ttcaggcgag cctgttcgaa gatggggcga aactgttcat tctctcgttt
     1021 caaggcctcg gtctgatgat agatcgccga gcgattcacc cggacaaacg ccagcggcgc
     1081 aatcaggaag aagaccatga acatcatcgc cagacgcggg gtcatggtaa accgcaagaa
     1141 cggcggccga ccttcgacga gactgatcaa ccccttttcg agcgctccgg cgagccaggc
     1201 accgtctgcc aggcccggag cgggcgcgcc gtcagtcttg gccccggccg tcagcacctg
     1261 cacgggcatc ttgaaggtct cttcaagcag cggcttgata ccggtgactt cgagatacgg
     1321 catgctcaga tagatcgtct gctcgccctg ctctttctct ttggcgcgaa tgtaggcgaa
     1381 gaacggcgtg atgcgggcca gcagctcgcg ctgattctgc tgaatgatct cgatcgagtg
     1441 ctggtctgcc tgcgcggcaa cgccggtgag caggtcatcg acctcgttct gcttcaactt
     1501 gaggctggtc atcaaccgca gctcgacctc tttgagcgcg ccggaaagac tggtgcgcac
     1561 ggcggtcacc tgcccgtcac gcagaatcgc catgttgacg ctggtgtagc caagcgagat
     1621 gagacagact tgccgttccg tacgagcttt cggctgccgg gaccaggcgc cgatgagggc
     1681 ttcgatgccc gtggtcaccc ggttgagcgt gaaaccaaga gattcgaaga ggctctgcag
     1741 gcggtttacg aagatgcgat tgacgtagga aacaagatac tcgcggttct tcggatcatt
     1801 gccgaccagc ggtctcagcg cgaagaccat ctcgg
//
LOCUS       DAORHD010001089         1833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_82919_length_1833_cov_2.799775, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001089 DAORHD010000000
VERSION     DAORHD010001089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82919_length_1833_cov_2.799775"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /gene="eno"
                     /locus_tag="PKO06_18930"
     CDS             complement(<1..1029)
                     /gene="eno"
                     /locus_tag="PKO06_18930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNV71787.1"
                     /translation="MSMIESVHARQILDSRGNPTVEVEVVLEDGSFGRAAVPSGASTG
                     AFEALELRDGEAAYGGKGVLKAVKNVNETIAPEIEGLDATDQVEIDELMIKLDGTPDK
                     SNLGANAILGVSLAVAKAAAETCGLPLYQYLGGPSARVLPVPMMNILNGGKHAVDSTD
                     LQEFMVMPVGAPTFSEALRWCAETYQALKKVLSAKKYNVSIGDEGGFAPSLKSNEEAI
                     EVILQAIEKAGYKPGDDIMIALDPAASEIFEDGKYVLAKEGRTLSAPEMVDFYERWVA
                     KYPIISIEDGMAEDDWEGWALLTERLTDKIQIVGDDLFVTNTERLQKGIDEEVANSIL
                     IKLNQIGSL"
     gene            complement(1181..>1833)
                     /locus_tag="PKO06_18935"
     CDS             complement(1181..>1833)
                     /locus_tag="PKO06_18935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV71788.1"
                     /translation="LRDAWPPDSLVVRCPDKLTSQVLLGFLSARNIRGIDTARVVFVG
                     FGQGGVIAALAAERSAGRVHALGLVNTPARSVDRTLLSGTFRDSVNLRRLERFFDDIW
                     TDKYNDSDFVMNGRVECWRSWVVLSGEMTDRMARLSIPTLAVQGTADSLLPLLDIERF
                     RRAIGRRQLSQVHTAVGLRHDLRDEIPDPGQDKKAISPRFVTIVLEWLKLVAPPAG"
BASE COUNT          398 a          565 c          472 g          398 t
ORIGIN      
        1 aagcgacccg atctgattga gcttgatcag gatggaattc gcgacctctt cgtcaatccc
       61 cttctgcaac ctctcagtgt tcgtaacgaa aagatcgtcc ccgacaatct ggatcttgtc
      121 ggtcaaacgt tcggtgagaa gtgcccaacc ttcccagtca tcctcagcca tcccgtcctc
      181 tatcgagatg atggggtatt ttgcaaccca gcgctcgtag aagtccacca tttccggtgc
      241 agaaagtgtc ctgccctctt tcgcgagaac gtatttgccg tcttcaaaga tctcactggc
      301 agcggggtca agggcgatca tgatgtcatc gccaggcttg taacctgctt tttcgattgc
      361 ctgcagaatc acttcgatcg cttcttcgtt cgacttcaac gaaggggcaa aaccgccctc
      421 atcgcctatg gacacgttgt acttcttcgc gctgagcacc tttttgagag cctgatacgt
      481 ctcagcacac catcggagag cttcactgaa cgtgggagca cccaccggca tcaccataaa
      541 ctcctgaaga tccgtcgagt cgacggcatg tttcccgccg ttgaggatgt tcatcatggg
      601 cacaggaaga actcgagccg atggtccacc gaggtactgg tagagcggaa ggccgcaggt
      661 ttccgccgca gcttttgcca cggcgagaga aacgccaaga attgcgttcg ctcccagatt
      721 gctcttatcc ggggtaccat cgagtttgat catgagttca tcgatttcca cctggtcggt
      781 ggcgtcaagc ccctcgattt caggtgcaat cgtctcgttg acatttttga ccgccttcaa
      841 aacgcccttg ccgccgtagg cagcctcgcc gtcgcgcagt tccaaagcct caaacgctcc
      901 cgtggaagcg cccgaaggca ctgccgcacg gccgaacgaa ccgtcctcaa gcaccacttc
      961 aacttcgacg gtcgggttgc cgcgcgagtc cagaatctgg cgggcatgaa cactctcgat
     1021 catgctcatg gcgtatcacc tttctcgtgt atcgatggag acgaacgctg ttcccagaag
     1081 cacagttgtt tctgtgaacc agctgctgat tgtgctgcaa agtgaataaa gtgagcccgg
     1141 ccggatcccg gacaggtctt tactcagtag agatgagatc tcacccggca ggcggcgcca
     1201 caagcttcaa ccattcaaga acgatggtca caaatcgtgg gctgatggcc tttttgtctt
     1261 gacccggatc cggaatttcg tcgcgcagat cgtgtcgaag tcctacagcc gtgtggacct
     1321 gcgaaagttg ccgtctcccg atcgctcgtc gaaaccgttc aatgtccagc aggggcaaca
     1381 ggctgtccgc cgtgccctgc acggccagcg tcggaatact gagcctcgcc atgcggtcgg
     1441 tcatttctcc cgaaagcaca acccaggatc gccagcactc cacacggccg ttcatcacga
     1501 aatccgaatc gttgtacttg tccgtccaga tatcatcgaa aaaccgctcc aaccggcgga
     1561 ggttcaccga atcgcggaac gttccggaga ggagtgtcct gtccaccgat cgagccggcg
     1621 tgtttaccag tccgagtgcg tgcacacggc ctgcggaacg ctctgcggcc agtgccgcga
     1681 taaccccacc ctgaccgaac ccgacgaata ccacccgggc cgtatcgatt ccccggatat
     1741 ttcgcgcact caaaaaccct agcaatacct gcgaggtcag tttgtcagga caccgaacaa
     1801 cgaggctgtc aggaggccac gcgtcccgca gag
//
LOCUS       DAORHD010001090         1831 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83058_length_1831_cov_3.088401, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001090 DAORHD010000000
VERSION     DAORHD010001090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83058_length_1831_cov_3.088401"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..>1831)
                     /locus_tag="PKO06_18940"
     CDS             complement(25..>1831)
                     /locus_tag="PKO06_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71789.1"
                     /translation="MAIGGVLFRLARWKKPPALMLRGKMIIGGLMLTAPPVLAMLIFG
                     YAQSRLMNEAALLASESRLDQRLQEIETAFVDAMFGLQTSLRQLTREPFMIRGDDESQ
                     IKAYLQKLIPWMGFRYYFYHRSGRHFSFAPNTDDPARNEHLRYISTLLKSAKFDFPLD
                     KISHAGAGALTNIMSGFEMPESKWEDVVGRLAPSEVGDSDWFAFPAFVYDHAGTKAAF
                     LFFSLQRRLMRDDFLRRQAERPITDVECLIDGHSQLGPAHRLLLDRATSSGRTQFGEF
                     RIQGERWQLLARPLRGLGVSAVAAVKIEKAALIDPYHGLQILGLAAMLVVSLGVMLFL
                     RWSMYEPVTRIRESISRIEAGEYGSLPEAHTADELEELAQAVGDLGRGLEQKARLQSF
                     VRPDLVASLTESRSAVARVSVAVLFAGLRDFSHLETALTPEQALETMGQFLSRVETAV
                     DRNGGQIDKFIGDTAMALFLPAQDHEAGEVRVRRAAVQIDAAMNLWNEERQSVGLPRL
                     CFGVGLASGETLTGSIGSRRRRLDFTAIGDVVNLSARLEKLAGRANGPSIMGTGVVCQ
                     QTEPGWFWVSTGIQEVRGRTGKTPVFGLIREDRCS"
BASE COUNT          415 a          578 c          502 g          336 t
ORIGIN      
        1 tactcgccgc acgcatcgta tacctcatga gcagcggtcc tctcgaatca gaccaaagac
       61 gggtgtctta ccggtccggc cgcgaacttc ctggataccg gtgcttaccc agaaccaccc
      121 cggctcggtc tgctgacaga cgactcctgt acccatgatc gacggcccat ttgctcgtcc
      181 agcaagcttt tccagtcgcg ccgacagatt cacgacgtcg ccgatcgccg tgaaatcaag
      241 ccggcgccgg cgcgaaccga tgctgccggt cagcgtttct ccggatgcca gaccgacgcc
      301 gaaacacagc ctcggcaacc ccacactctg ccgctcctcg ttccagagat tcatggcagc
      361 atcgatctga acagccgcgc gacgaacgcg cacttctccc gcctcgtggt cctgggcggg
      421 cagaaagagc gccatcgcgg tatcgccaat gaatttgtcg atctggcctc cgtttcggtc
      481 gaccgccgtc tcgacccgac tcaggaactg tcccatcgtc tcgagcgctt gttccggtgt
      541 cagagcggtt tccagatgcg agaaatctcg aagtccggca aacagcaccg cgaccgagac
      601 tcgcgcgacg gcggagcgac tctcggtgag agaagccacc agatcaggcc gaacaaacga
      661 ctgcaaccgg gctttctgct cgagcccccg gccgagatca cccaccgcct gagccagttc
      721 ctcgagttcg tcggcggtgt gcgcttcggg cagagaccca tattcccctg cctcgatccg
      781 tgagatggac tcgcggatac gcgtcacggg ctcatacatg gaccaacgca ggaacaacat
      841 gactccgagc gaaaccacaa gcatggccgc cagtccgagt atctgcagac cgtgatacgg
      901 atcgatcagc gcagctttct cgatcttcac cgccgcaacc gcggacactc ccaggcctct
      961 cagagggcgt gccagcaact gccatctctc accctgaatc cgaaactctc cgaactgggt
     1021 acgccccgac gaggtggctc gatcgagaag cagacgatgc gcaggtccga gctgagagtg
     1081 accgtcgatc aagcattcga cgtcagtgat cgggcgttcc gcctgtcgac ggagaaaatc
     1141 atcacgcatc aaacggcgct gtagactgaa aaagagaaaa gccgcttttg taccggcatg
     1201 gtcgtagacg aaggccggaa atgcgaacca gtcggaatca ccgacctcag aaggcgcgag
     1261 acgaccaacc acgtcttccc atttgctttc gggcatttcg aacccgctca tgatgtttgt
     1321 caaagccccg gcaccagcat gggagatctt gtcgagcgga aaatcgaact tggcactctt
     1381 gagcaatgtc gaaatgtagc ggagatgttc atttcgagcc ggatcatcgg tattgggagc
     1441 gaacgaaaag tggcgaccac tgcggtgata gaagtagtac cgaaaaccca tccacggtat
     1501 gagcttctga agataggcct tgatctggct ttcatcgtca ccccggatca tgaagggttc
     1561 gcgcgtcaac tgtcgcaatg atgtctgcaa tccgaacatg gcgtcgacaa aggctgtctc
     1621 gatctcctgc aggcgctgat cgaggcgcga ctcactggcc agcagagcgg cttcgttcat
     1681 gagtcgagac tgagcatagc cgaagatcaa catcgccagc actggaggag ccgtgagcat
     1741 gagaccaccg atgatcatct ttccgcgcag catcaaggcc ggaggtttct tccagcgagc
     1801 cagccggaac agcacccctc cgatagccat g
//
LOCUS       DAORHD010001091         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83134_length_1830_cov_3.241690, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001091 DAORHD010000000
VERSION     DAORHD010001091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83134_length_1830_cov_3.241690"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>1830
                     /locus_tag="PKO06_18945"
     CDS             297..>1830
                     /locus_tag="PKO06_18945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNV71790.1"
                     /translation="MPWFSYPALRASGRTDRRGHVGDFDVVLVSGDAYVDHPSFPTAV
                     IARLLEHAGLTVAVIAQPDWQSEEDIRAFGRPRLFFGVTAGSMDSMVANYTSMRFPRK
                     EDRLSPAGRAGLRPRRAVMVYTQLVRRCFPGTPIVLGGIEASLRRFMHFDYWENRLRD
                     PMLVDAPADLLIYGTAEAPLLRLVRELQGGRSFSEAARAGLPQTCLRVPFGQGPELAG
                     SRAQLLPSAAECRTDPRAFLRLSLALDTAVRPNGPVLVQPHPKGDIVCFPPTLEMHVE
                     EIRLLSALKFNRRAHPLYTEPIPGLIPVQFSIQSHRGCAGACTFCALAIHQGRRIRSR
                     SIDDLVREAKAFPTHPDFRGTIPDLGGPSVNMYGWDCVNDGCDSGVCTHPKRCPNLRG
                     GLKPLVEALQAVAAIPGVRHVFLGSGLRYDLIRENDWDDFAIILRHHISGQLKVAPEH
                     VSPAVLRLMRKGVGANFPQFVDRFRELTARLRLPLFLVPYFMTAFPGSVDEDSLLSQL
                     VRE"
BASE COUNT          268 a          568 c          562 g          432 t
ORIGIN      
        1 tgttctgaac gggcagtatc tggtcgctcc gctctacctg ctcgataaag tgcgctactc
       61 ggctctcttc gtcatgccgt ctgccgttcc gcccgagggc gcgatcgatc cccccgctca
      121 gccttgagtg gttctgagct ctcccggcga gcgaggcaaa gtgtttcgag agacatgtct
      181 ggcatgatca tttcgcttgg ctgttttgcg gtctgctgtg gtgggttctg gggcaacagg
      241 ctcatggtgg gggtgcgggg gcgaagctcc tgtgtccctc ttgaaagaac cacctgatgc
      301 cctggttttc gtatcccgcc ctgcgtgcct ccggtcgtac tgatcgtcgc ggtcacgtcg
      361 gagatttcga tgtcgtcctg gtcagtggtg acgcctacgt cgatcatccc tcgtttccga
      421 ctgctgtcat tgctcgtctg ctcgaacatg ccggtctgac ggtggcggtg attgcccagc
      481 ctgattggca gtcagaagag gacattcgtg ccttcggccg cccgcgcctg tttttcggcg
      541 tgaccgccgg gtcgatggac agcatggttg ccaactacac ctcgatgcgc tttccgcgca
      601 aggaagatcg cctgtcaccc gctggccgtg cgggtcttcg acctcggcgg gccgtcatgg
      661 tctatacgca gctggtgcgc cgctgcttcc cgggcacacc gattgttctg ggcggcatcg
      721 aggcttccct gcgccgattc atgcatttcg actactggga aaaccgcttg cgcgacccga
      781 tgctcgtcga tgctcccgcc gacctgctga tctacggcac ggctgaagcc ccgttgttgc
      841 gcttggtgcg cgaactgcag ggcggtcgat cgttctctga ggccgcccgc gccggtctgc
      901 cgcagacgtg cctgcgcgtg ccgttcggcc agggccctga gctcgccgga tcgcgggcgc
      961 aattactgcc gtcggctgcc gagtgccgca ccgatccgcg cgcgttcctg cgcttgtcgc
     1021 tggctctcga tacggcggta cgtccgaacg gaccggtact ggtgcagccg catcccaagg
     1081 gggatatcgt ctgctttcct ccgacgctcg agatgcatgt cgaagagatt cgtctgttgt
     1141 cggcattgaa gttcaatcgg cgcgcgcatc ccttgtatac cgaaccgatt cccggtctga
     1201 tcccagttca gttctcgata cagagccatc gtggttgtgc cggagcttgt acattctgtg
     1261 cgctggccat tcatcagggc aggcggattc gctcgcgatc gatcgacgac ctggtgcgcg
     1321 aggcgaaggc gttccccacg catccagatt tccgcggcac gatccctgat ctgggcgggc
     1381 cctcggtcaa tatgtacggg tgggattgcg tgaacgatgg ctgcgatagc ggggtctgca
     1441 cgcatccgaa acgttgtccg aatctgcggg gcggtctgaa accgctggta gaggccctgc
     1501 aggcagtcgc cgccattccc ggggttcgac atgtgttcct tggttccggt cttcgctacg
     1561 atctcatccg cgaaaacgac tgggacgact ttgccatcat cctgcgacac cacatctccg
     1621 ggcagttgaa agtggcaccc gaacacgtgt caccggccgt gttgcgtctg atgcgcaaag
     1681 gggtgggggc gaacttcccg caattcgtcg accggtttcg tgagctgacg gctcgccttc
     1741 gactgccgct gtttctcgtg ccttacttta tgaccgcctt tcccggctct gttgatgaag
     1801 attcgcttct ctcgcaactg gtacgcgaac
//
LOCUS       DAORHD010001092         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83284_length_1828_cov_3.357022, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001092 DAORHD010000000
VERSION     DAORHD010001092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83284_length_1828_cov_3.357022"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="PKO06_18950"
     CDS             <1..829
                     /locus_tag="PKO06_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71791.1"
                     /translation="QGLLTHEKWMQLNPPAGKYWVIKDGAIETADKPNTPITEGNTTP
                     AGNTSAPAGTTPANSGTTTPSTPKPEPKPEVKPEPAKPVDEYIKLFQADLKNMGIKDI
                     SGMKDSAYYNALNKARGLVAGSWWQSNPMDKFLMNDYKNIWSLQKQLLDAHKTYQEVW
                     EKRRTSKILGIFGNDIESAGKRVDQLKARLQEAWNEFLPVYDKAKAAAEEAKKNVSAK
                     QEEIKKLEADFAKLGRDPSKAAEVDRIATAIENARDQIKDDEAKVQQFEPMKALFGR"
     gene            932..1771
                     /locus_tag="PKO06_18955"
     CDS             932..1771
                     /locus_tag="PKO06_18955"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HNV71792.1"
                     /translation="MLLSAMLGAALVACSFSMAMAAPDEAPTEGRILFVDTSEKDQTY
                     LATMNPDGSGKTRLTPGYSNIVFPRYCETSGWMGFTNKLPDMKSEVYLLARDGKKIKK
                     ILDGAALEGFSPDGKFLLYTTCDQAAGLYSYSLDSKTATKLSEDMQVTAADWSATGNW
                     IAVSALTSKGTSDIWLISTKAQGIVRITDTPTISESFPVFSKDEKYLAVITDRHGQSE
                     LEYIDLDKREFQRPLLMGLYPSLSPSNRWITFETGDEVAVARADGINIKMLSKGRTPV
                     WIK"
BASE COUNT          461 a          535 c          492 g          340 t
ORIGIN      
        1 tcagggcctg ctcacccatg agaagtggat gcagctgaat ccgcccgccg gcaaatactg
       61 ggtgatcaaa gacggagcca tcgagacggc agacaagccg aacaccccga ttaccgaagg
      121 caacaccacc ccagccggga acacctccgc tcccgccgga accacacctg caaattctgg
      181 gaccacgact ccgagcactc cgaagccgga acccaagcct gaagtcaagc ccgagccggc
      241 caagcccgtt gacgagtata tcaagctctt ccaggccgac ctgaaaaaca tggggatcaa
      301 agacatctcc ggcatgaagg acagcgccta ctacaacgcc ctcaacaagg cccgcggtct
      361 ggttgccggc agctggtggc agtccaaccc catggacaag ttcctgatga acgactacaa
      421 gaacatctgg tcactccaga agcagctgct cgacgcgcac aaaacctacc aggaagtctg
      481 ggaaaagcgc cgcaccagca agattctggg catcttcggc aacgatatcg aatccgccgg
      541 caagcgcgtg gatcagctga aggctcgcct gcaggaagcc tggaacgagt tcctgccggt
      601 gtatgacaag gccaaggccg cggcagaaga agccaagaag aacgtttcgg ccaaacagga
      661 agagatcaag aagctcgaag ccgacttcgc caagctcggt cgcgatcctt ccaaagccgc
      721 cgaagttgac cgcatcgcaa ccgcgatcga aaacgctcgc gatcagatca aggacgatga
      781 agccaaggtt caacagttcg aaccgatgaa ggcattgttc ggccggtaaa atccggttgg
      841 cataaaccag aacgttggtt tatacttcgg gcggggatct cccgcccgat tttcttttct
      901 acagaaaggc aggtatgaag gcgtgaaacg aatgcttttg tctgcaatgc ttggtgctgc
      961 tttggtggcg tgttccttct cgatggcaat ggccgccccg gacgaggctc cgaccgaagg
     1021 ccggattctt ttcgtcgaca cgtcagagaa agaccagacc tatctggcca cgatgaaccc
     1081 tgacggttcg ggcaagactc gcctgactcc cggctattcc aatatcgtgt ttccccgcta
     1141 ctgcgaaact tcagggtgga tgggcttcac gaacaaactg ccggacatga aatcagaagt
     1201 gtatctgctc gcccgtgacg ggaagaagat caagaagatt ctggatggcg cagcactcga
     1261 agggttttcg ccagacggca agtttctgct gtacaccacc tgtgatcagg ctgccgggct
     1321 gtattcctat tcgctcgatt cgaagacggc caccaagctg tctgaagata tgcaggtcac
     1381 cgccgccgac tggtcagcga ccggcaactg gattgccgtt tccgctctga ccagcaaggg
     1441 aaccagcgac atctggctga tttccaccaa agctcagggc atcgtgcgca tcaccgatac
     1501 gccgacgatt tcggaatcgt ttccggtctt ttcgaaagac gagaagtatc ttgccgtcat
     1561 caccgatcgt catggacaga gcgaattgga gtatatcgac ctggacaaac gcgaatttca
     1621 acgccccctg ctgatgggat tgtatccatc gctgtcgccc tcgaatcgct ggatcacctt
     1681 cgaaaccggc gatgaagtgg ccgtggctcg cgccgacggc atcaacatca agatgctgag
     1741 caaaggccgc acccctgtct ggatcaagta accagcgaaa ggaccccgca tgatacagct
     1801 gaagaagatt ctggttcccg ttgatttt
//
LOCUS       DAORHD010001093         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83285_length_1828_cov_3.324873, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001093 DAORHD010000000
VERSION     DAORHD010001093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83285_length_1828_cov_3.324873"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKO06_18960"
     CDS             <1..289
                     /locus_tag="PKO06_18960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HNV71793.1"
                     /translation="MVGLGLENEVRHVVETYGWQAPGLRSIGYAEWREYFEGERSRAE
                     VTQLIQQHTRQFAKRQLTWFRHQITGTIVQPDDSASTERFFEQVQEFYLAS"
     gene            608..>1828
                     /locus_tag="PKO06_18965"
     CDS             608..>1828
                     /locus_tag="PKO06_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71794.1"
                     /translation="MPEVTDLQINTGVRRELSGRRVDLSKLKVTVSGGVVTLAGEWAF
                     VGLTKTQEESSVEMKFLESCLRNLRGVKDLVFQFDNWVKNDTGKWEPKTGSGSAGSAA
                     SGAGGEGMNCPSCKTVIKFCPCCGEPLVPGLGEAHSRVAVAGRPVLKGLPPRKQETTR
                     TLLKPLPPVKLGEPRMKPEGGPPDAPAERTGSGAFTPIRPSTPTPESKIKPLPVPPKL
                     APVTPPIQTSTPLSPLKPLPPVQPHLPPKIPVAEPKAESPLKPVPGVVSPLVKPVVPV
                     AKPVTSLVKPVAEPVTPVPSPITKDEEPTEDLLLSLEPPAHSELSLDPLGAKKALPAS
                     APSGETPRSPELVPASEALPLPPKPGHSPAVSETKSDDAEALKSTPAVPGSARGETLI
                     PLKKPGMVAKQPSIP"
BASE COUNT          415 a          517 c          536 g          360 t
ORIGIN      
        1 gatggtcggc ctgggtctgg aaaacgaagt tcggcatgtc gttgagacct acggttggca
       61 ggcccctggg ttgcgaagca tcggctatgc ggagtggcgg gagtattttg agggagagcg
      121 aagccgtgca gaagtaactc agttgattca gcaacatacc aggcagttcg ccaagcggca
      181 gctgacctgg tttcgtcacc agataaccgg tacgattgta cagccggatg actctgcatc
      241 aaccgaacgc tttttcgagc aggtgcagga attctatctc gccagttgac ggaaaattgc
      301 tcgaaccggt tgggttgccg ggagatcgtg ccatggtctg atgcctggaa gctcaagcct
      361 ccgtcaccgc gagaagggtt ctcgtatgcc cgtaaagagc cgctatcggg ctggtttccc
      421 ggaaatatca tggtatactc tcaagtgttt cgaaaattgt gccgacaata gacgttggaa
      481 aggtaggact gtcttccttt cccggcacgc acaaggaaag tgatgttgtt ccggggtgcc
      541 ggttcgaaac gcccctgccg gatctgtgac aatctggggc acagacggct gattgcggag
      601 gtacaccttg cccgaagtaa ccgatttgca gatcaacacc ggtgttcgac gtgaactttc
      661 cggacgcaga gtcgacctga gtaagctgaa ggtcaccgtc tccgggggcg tggtgacgct
      721 tgcgggtgag tgggcgtttg tcggattgac gaagacccag gaagagtcat ccgtggagat
      781 gaagttcctg gaatcctgcc tgcgcaacct tcgcggcgtg aaagacctgg tattccagtt
      841 tgacaattgg gtgaagaatg acacgggcaa gtgggagcca aagacaggga gcggatcagc
      901 aggttccgcg gcgtcaggtg ccggcggtga gggcatgaat tgcccatcct gcaagaccgt
      961 tatcaaattc tgcccctgct gtggagaacc attggttccc gggctcggtg aagcgcattc
     1021 acgtgttgcg gttgccggtc gtcccgtgct caaaggactc ccgccccgca aacaggaaac
     1081 gactcgaaca ctgttgaagc ccttgccacc ggtgaagctc ggtgaacccc gcatgaagcc
     1141 agaaggcggg ccaccggatg ctccggccga gcgcacggga agcggagcct tcacaccgat
     1201 tcgaccatcg acaccaacgc cagagagcaa gatcaaaccg ctgccggtgc cgccgaagct
     1261 tgctccggtc acacctccga ttcagacatc gactccgtta agtccgctga aaccgctgcc
     1321 accggtacag ccgcatctgc caccgaaaat cccggttgct gaaccaaaag cggaaagtcc
     1381 gctcaagcct gttccaggcg ttgtttctcc gctcgtgaaa cccgttgtac cggttgcaaa
     1441 acctgtgacg tctttggtga agcctgttgc cgagccggta actcccgtcc cctcgccgat
     1501 cacaaaagac gaggagccga cagaggatct gctgttgtcg ctcgagccac ccgcacactc
     1561 agaactatca ctcgatccat tgggggcgaa gaaagcgttg ccagcgagcg cgccgagcgg
     1621 tgaaacacca cgatcacccg aacttgttcc cgcttctgaa gctcttcctt taccgccaaa
     1681 accaggacat tcgccggcgg tatctgaaac caaaagtgat gacgccgaag cgttgaagag
     1741 cactccagca gtcccgggtt ccgcccgagg cgaaacgctc attccgctca agaaacccgg
     1801 gatggttgcg aaacagccca gcatacct
//
LOCUS       DAORHD010001094         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83331_length_1827_cov_3.972348, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001094 DAORHD010000000
VERSION     DAORHD010001094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83331_length_1827_cov_3.972348"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="PKO06_18970"
     CDS             <1..1514
                     /locus_tag="PKO06_18970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71795.1"
                     /translation="TGAPWQVGDAIVLPCEMAVIEEDSNVGSDIPSQTLKRMVMVLQI
                     PNSMFPQSPLRIGDIDTYRVLNRIGSYTRSVAREYVLRAADKDVFEAQVPKVFQNAFP
                     SVGSAGTKVGDDQIIRALDSIGPLQTVDYNKAGNAFIVDMLRKWRPRGFDLTPDEMEN
                     ISGMASFALGVKECDPRETCRDMFTICQQFSEWKLVDSIPPPDRGTKAWNIFSVWVPK
                     YPGPDGFPTQYPLKPTWDYFEKTLGREIRQLLYPNVPVGANQTPPYVFNQSRFQNPTD
                     EFLDFRNFKGPYAPFAKRSDGGYDSYKDNVSPEEFVRRMSVLKNAAKRLTFTFLRWIP
                     WQDWDYQDMLTSYLNGNPLTHENVVGFDAANYWPWWGDQRQIVADRSKFNWPKAKDVY
                     GSDTFGAKGWPDQLRQPIGDPNGPHINPSSIKPADLVSEVDVPYTYELKFKNGTGIRT
                     RLDYLLDYFRKYFDEPELNPDGKKIRGANEQTDTPQVPGPPDLLGAAYTGLSS"
     gene            1526..>1827
                     /locus_tag="PKO06_18975"
     CDS             1526..>1827
                     /locus_tag="PKO06_18975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71796.1"
                     /translation="MRTSRDAFSLIEVLMAIMVFSIACLPLYQLYFKTGISQQRMIRD
                     FIAVTNVAEKIVNRIDHQLEKKPKLLSPMQKDVTPQILVGMEDEEDWNFLGKAFAD"
BASE COUNT          404 a          544 c          508 g          371 t
ORIGIN      
        1 ataccggagc cccctggcag gtgggtgacg ccattgtgct gccctgtgaa atggcggtca
       61 tcgaagaaga ctcgaacgtc ggctcggaca ttcccagcca gacgctgaaa cggatggtca
      121 tggtgttgca gattcccaac tcgatgttcc cgcagagccc tcttcgcatc ggagatatcg
      181 atacctaccg cgttttgaat cgtatcggct cgtacacccg cagcgtggcg cgtgagtatg
      241 tgttgcgggc ggccgacaag gatgtgttcg aggcgcaggt gccaaaagtc tttcagaatg
      301 cctttccgtc ggtcggctcc gccggcacca aagtcggtga cgatcagatc attcgtgctc
      361 tcgacagcat cggaccgctg cagacggtcg attacaacaa ggccggaaat gccttcatcg
      421 tcgacatgct gcgcaagtgg cgaccacgtg gcttcgacct cactcccgac gaaatggaaa
      481 acatctcggg catggcgtcg tttgccctcg gcgtgaaaga gtgcgatccg cgcgaaacct
      541 gccgcgacat gttcacgatc tgccagcagt tttccgagtg gaagctcgtc gattcgattc
      601 cgccgcctga tcgcgggaca aaagcctgga acatcttttc cgtctgggtc ccgaaatacc
      661 ccggccctga tggctttccc acgcagtatc cgctgaaacc gacctgggac tatttcgaga
      721 agaccctcgg tcgtgagatc cgacagttgc tgtatccgaa cgtcccggtc ggcgcgaacc
      781 agaccccgcc atatgtcttc aatcagtcgc gtttccagaa tccgaccgat gagtttctcg
      841 attttcgcaa tttcaagggt ccgtatgctc ctttcgccaa gcgctcagac ggcggatatg
      901 actcgtacaa agacaacgtg tcccccgagg agttcgtgcg gcgcatgtcg gtgctcaaaa
      961 atgccgcgaa acgcctgacc ttcaccttcc tgcgctggat cccctggcag gactgggatt
     1021 accaggacat gctcacctcg tatctgaacg ggaatccgct gacgcacgaa aacgtcgtcg
     1081 gcttcgatgc cgccaactac tggccgtggt ggggcgacca gcgccagatc gtcgctgacc
     1141 ggtcaaagtt caactggcca aaagcgaaag atgtgtatgg aagcgacacg tttggcgcga
     1201 aaggctggcc tgatcaactg cggcagccga tcggcgatcc caacggacca catatcaatc
     1261 cgtcgtcgat caagccggcc gacctcgtgt ccgaggtcga tgttccctat acgtatgaac
     1321 tcaagttcaa gaacggcacc ggcatacgca cccgactcga ctatctgctc gactacttca
     1381 ggaaatactt cgatgagccg gagctcaacc ccgacggcaa gaagattcgc ggcgcgaatg
     1441 aacagaccga taccccgcag gtgccgggcc cgcccgatct gctgggcgcg gcctacacgg
     1501 ggttaagctc atgaagcggc agatcatgcg cacgtcccgt gatgccttca gtctgatcga
     1561 agtgttgatg gccatcatgg tgttttcgat cgcctgtctg ccgctctatc aactgtattt
     1621 caagacgggc atcagtcagc agcgcatgat ccgtgacttc atcgccgtga ccaacgtggc
     1681 cgagaagatc gtcaaccgca tcgatcacca gctggagaaa aaaccgaaac tgttgagtcc
     1741 gatgcagaag gacgtcacgc cgcagattct cgtcggcatg gaagatgaag aagactggaa
     1801 cttcctgggc aaggcctttg ccgacga
//
LOCUS       DAORHD010001095         1825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83507_length_1825_cov_3.166102, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001095 DAORHD010000000
VERSION     DAORHD010001095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83507_length_1825_cov_3.166102"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="PKO06_18980"
     CDS             <1..1257
                     /locus_tag="PKO06_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71797.1"
                     /translation="APPAPPTPITIPGAPPPAPLSTIPRTTEPSVSEAPTRKPVAAPS
                     QPTDRILSQIERLRPQLPDMLAKWKGQEKILLIKSLARMGRREDAQLLQPCLSDGDPA
                     CLASTIEALSLLDPDILFPFLPRLIQHAADEVRVVAIRAFSLFDKRQALALVEKMLFA
                     VQPRQRNLAIFCAAELDFPSVRHLLLKAIQREQDPDNFKQICAILHNNPDEELYEQLF
                     ELAGELDEFKRDTLKQLLSEMADHLVRTHETSHRSVSELEKSVRQRIAEEKEKRTKAQ
                     PSYSLQNIQKIRQQQTSAAQGLFSDPGLVRFAASAFSTGALLTIILWFFVVAPLAEEP
                     TRIVIKRSTGKDANKTPNIKGQVTGVAAGGLALYLQIEGETVPYYVQFRGGSPKVFKP
                     GDPFQGQIRLMKTDGQPKVGEVVFSY"
     gene            1349..1696
                     /locus_tag="PKO06_18985"
     CDS             1349..1696
                     /locus_tag="PKO06_18985"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV71798.1"
                     /translation="MATFICSSEEVKDVFVVRCNGYLDDKGGSQVRNTVENSFAKGFQ
                     KVVLNFSDSPVINSQGISHIIEIAELVVEDRQGKLAFVGLSELANSVFKTVGLLRMAE
                     AFPDEDEALRVLL"
BASE COUNT          410 a          554 c          474 g          387 t
ORIGIN      
        1 gcgccgcccg cccctccgac gcccatcaca attcctggtg cccccccccc tgctccactc
       61 agtaccatcc ccaggacaac cgagccatcc gtttccgagg cgccgacacg caaacccgtc
      121 gcggcgccat cacagccgac agatcgcatt ctgtctcaga tcgaacgttt gcggcctcaa
      181 ctgccggaca tgctggccaa gtggaaaggg caggaaaaga tcctgctgat caagtcactt
      241 gcccgaatgg ggcgtcgcga agacgcgcag ctgctgcagc cctgtctcag cgatggcgac
      301 cctgcctgtc tggcgagtac catcgaagcc ctcagtctgc tcgatccaga catcctgttt
      361 ccgtttctgc cgcggctgat tcagcatgct gctgacgagg tgcgggtcgt ggccatacgg
      421 gccttctcgc tcttcgacaa acggcaagcg ctggcacttg tcgaaaaaat gctgttcgcc
      481 gtacagcctc gtcagcggaa tctggccatt ttctgcgccg ccgaacttga ctttccatct
      541 gtccgacatc tgcttttgaa agccattcag cgcgagcagg acccggacaa tttcaagcag
      601 atatgcgcca ttcttcacaa caacccagat gaagaattgt atgagcagct gtttgagctt
      661 gccggcgaac tggacgaatt caaacgcgac actctcaaac aactcctgtc tgagatggca
      721 gaccacctgg ttcgcacgca tgagaccagc caccggtccg tctccgaact tgaaaagagt
      781 gttcggcagc gcattgccga ggagaaagaa aagcgcacca aggcacaacc cagttactca
      841 ctgcagaaca ttcaaaaaat ccgacaacaa cagacatcag ccgcccaagg gctcttttcc
      901 gatcccgggc ttgtacgttt tgccgcatca gcgttcagta ccggagctct tctgacgatc
      961 atcctgtggt tcttcgttgt cgcccccctc gccgaggaac cgacccgaat cgtcatcaag
     1021 cgatcgaccg ggaaagacgc gaacaaaacg ccgaacatca agggtcaggt gaccggcgtc
     1081 gccgctggag gactcgcgtt gtatctgcag atcgagggcg aaaccgttcc ctactatgtt
     1141 cagttccgag gcggatcacc caaggttttc aaacccggcg atccgtttca ggggcagatt
     1201 cgtctgatga aaaccgacgg acaacccaaa gtgggcgaag ttgttttttc ctactgaggc
     1261 tcctcatcac gcgtgtgaag caatcccact ctctcgtcgt tggtgcccgg agtttgactt
     1321 catccgcatc aatgagtaag atgccctcat ggccaccttt atctgttcaa gcgaagaagt
     1381 gaaggatgtt ttcgtcgtac gctgcaatgg gtatctcgac gacaagggtg gcagccaggt
     1441 tcggaacacg gtcgagaact cgtttgccaa agggttccag aaagtcgttc tgaacttcag
     1501 cgattctccg gtgatcaaca gtcagggaat ttcgcatatc atcgagattg cggaactcgt
     1561 ggtcgaagac cgtcagggga aactggcttt cgtcgggctc agcgaattgg ccaacagcgt
     1621 cttcaagacc gtcggcctcc tgcgcatggc cgaagcattc cctgatgagg atgaagccct
     1681 tcgcgttctt ctctgactcc actcgctcgc gtggctctca cgcagaaacg taggcaatga
     1741 caaaagccgg cctgcaacgg ccggcttctg atttcgggtt cattcaggta aacaggtcgc
     1801 ttcgcggcgt atcgacggta attcc
//
LOCUS       DAORHD010001096         1824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83567_length_1824_cov_3.826456, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001096 DAORHD010000000
VERSION     DAORHD010001096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83567_length_1824_cov_3.826456"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKO06_18990"
     CDS             complement(<1..246)
                     /locus_tag="PKO06_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71799.1"
                     /translation="MLHHVEIYVSDLSRSRAFWSCFLGELGYQLYQEWPQGFSMLAGS
                     TYLVFVQVEDRFKTPDFHRCRVGLNHLAFHARDRQQVD"
     gene            complement(401..781)
                     /locus_tag="PKO06_18995"
     CDS             complement(401..781)
                     /locus_tag="PKO06_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71800.1"
                     /translation="MRKTLVLLAFILLVVGSIPAFALDEKQMRDDMYSFAAITAYLDV
                     VMHPGVPSSAKDYAMIGKQLDELDGIKQRLYKQIMSLEAGSEFEKARTVITEFKAMHG
                     FEKEVGHFVGLWLTEREKFLSIQN"
     gene            complement(930..>1824)
                     /locus_tag="PKO06_19000"
     CDS             complement(930..>1824)
                     /locus_tag="PKO06_19000"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV71801.1"
                     /translation="GLIAISPPFFSCFSALAVSRLLRTRYILDIQDLYPETAVHLGVL
                     QNRFLIKVFEAAERYLYRQSSGVVGISNGFADHFKQAGVAAARTCVIPNWVDLQAFPY
                     QTCRHDAPTAQHPLRLGFFGNHGLAQGLETLIEGMALLRDEPRVHLTLIGDGVAKPDL
                     IALARNRHLDNINFESPCAHEQVADKLRSIDVGIVHLRHNPLYRITVPCKTYEYMALG
                     KPILIGVDGEARRVVEDHRAGLFFEPERPEQFARVVRELIPFSSRLPVFSKCGRNAVE
                     QQYSREILGHRYLQFLTERFS"
BASE COUNT          454 a          455 c          548 g          367 t
ORIGIN      
        1 gtcgacctgt tgtcgatcgc gcgcatggaa cgccagatga ttcaagccca cccggcaacg
       61 atggaaatca ggggttttga agcggtcttc gacttgtaca aacaccagat aggtcgaacc
      121 cgccaacatg ctgaatccct gtggccactc ctggtagagc tgatagccaa gctcgccgag
      181 aaaacaggac cagaacgcgc ggctgcggga aagatcggaa acatagattt cgacatgatg
      241 gagcaggcca ggagatgggc ggggtgagat gtttgaattg gtcatggaat ggctctcatt
      301 tcagcgggaa tgggaaacag cctgctggtg caggctgttt cgtgggggag ggcaaagcgg
      361 tctgagtgaa aagagggagg gctctatcac tctgttgatg ctagttctga atgctgagga
      421 acttctcgcg ctctgtgagc cagagaccga cgaagtggcc gacttctttc tcgaagccat
      481 gcatggcctt gaattcagtg atgaccgttc gggctttctc gaattctgag ccagcctcga
      541 ggctcatgat ctgtttgtac aaacgctgtt tgatgccgtc gagctcgtcg agttgcttgc
      601 cgatcatggc gtagtccttc gcgctggagg gcactccggg gtgcatgacg acatcgagat
      661 aggccgtgat tgctgcaaaa ctgtacatgt cgtcgcgcat ctgcttctca tcgagtgcaa
      721 aagccggtat cgaaccgacc accagaagaa tgaatgccaa cagaaccaga gtttttcgca
      781 tggtttgctc ccatctcgag gaatttgaaa aaagggacaa cgcaaggccg atgccaacat
      841 caaaaatggc tgtggtcgct gacgtatgcg atatcgcaca ggaagaagac ggttttctgg
      901 cagatcgacg tcaggaaacc ggcacgtggt caggaaaacc gctccgtgag gaactggaga
      961 taacgatggc cgagaatctc gcgtgaatac tgctgctcga cagcattgcg gccgcatttc
     1021 gaaaagaccg gcagccgcga ggaaaacgga atcagttcgc gaacgacccg ggcaaactgc
     1081 tctggtcgct cgggttcgaa aaagaggccg gcccgatgat cttcaaccac acgccgcgcc
     1141 tcaccgtcga caccgatgag aatcggtttc cccagagcca tgtactcata ggttttgcag
     1201 ggaaccgtga tgcgatacag gggattgtgc ctgagatgga cgatgccgac gtcgattgag
     1261 cgcagtttgt ctgcgacctg ctcatgagcg cagggtgatt cgaagttgat attgtccagg
     1321 tgacgattgc gtgccaacgc gatgagatca ggttttgcca caccatcacc aatcagggtg
     1381 agatggacac gcggttcgtc acgcagcaga gccattcctt cgatcagggt ttcgagaccc
     1441 tgtgccagac cgtgattacc gaagaacccg agtcgcagag gatgttgggc tgtcggcgca
     1501 tcatggcgac acgtctggta ggggaacgcc tgcagatcga cccagttcgg aatgacgcac
     1561 gtgcgagccg cggcaacgcc cgcttgtttg aagtggtccg caaacccatt cgagataccg
     1621 acaacaccgg aagactgccg gtacaagtat ctctcggcgg cctcaaagac tttgatgaga
     1681 aatcgattct gcagaacccc aagatgaacg gccgtttctg gatagagatc ctgtatatcc
     1741 agaatgtaac gggtgcgcaa cagacgagag acggcgagag ccgaaaaaca cgaaaagaat
     1801 gggggggaga tggcgatgag accg
//
LOCUS       DAORHD010001097         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83667_length_1823_cov_2.755090, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001097 DAORHD010000000
VERSION     DAORHD010001097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83667_length_1823_cov_2.755090"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..1186
                     /locus_tag="PKO06_19005"
     CDS             191..1186
                     /locus_tag="PKO06_19005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNV71802.1"
                     /translation="MIEINKPKIKYTEGPELNHGVLVMEPLERGYGTTLGNAFRRVLL
                     ASLPGSAVSSVKIDGVLHEFCSIPGVVEDVTEIILNLKKLIVSLKQDEPLTIRLEVKG
                     PCQVTAADLGSHPTLQIVDPSVHLCHVTGDINFGMDITFKRGRGYVLAEGHKESGQPL
                     GVILVDSQFSPVTKVNFNVEDARVGQEINFDKLTLQVWTNGSMKPHESVELAAKFLRT
                     HVDLFANLQEDEESVDEEVVTKSEDLVMVSQQKKEPTQKSYTDVLIKDLEFSVRSRNC
                     LKKAGIHNLADLVNKKASELLEIKNFGKKSLKEVREKLVQFNLTLPGDENGGGDV"
     gene            1183..1722
                     /locus_tag="PKO06_19010"
     CDS             1183..1722
                     /locus_tag="PKO06_19010"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HNV71803.1"
                     /translation="MKHQKIGRKFGRESHQRVALIRSLCTSLVKYERIETTLHKAKDL
                     RRVIERAVTMAKKDNAQKNPELANFFHAYGDKEIIGRDDIKRYLSNLKKEVREKVEKY
                     LADPAKAEKPEFVLEYLASTGDRAKGPRILRLEGVLTKLVKRIAPRFKDTNGGYTRIY
                     KLGCRRGDSAEMAVIEFTK"
BASE COUNT          451 a          526 c          499 g          347 t
ORIGIN      
        1 ctctgcgcaa gaccttcaag gatatgttcg aagcgggtgg ccgtgagaag agcccctggg
       61 tcgaagtcga tcccgaagcc ctcaaaggca agctcgttgc tctgcctgag cgcacgcaac
      121 tcgatccgaa gatccaggaa aacctgatcg tcgaatacta ctctcggtaa accacacgga
      181 ggcatacccg gtgatcgaaa tcaacaaacc caagatcaaa tacactgaag gccctgaact
      241 gaaccacggg gttctggtca tggaaccact ggaacgcggt tacggcacca cgctgggcaa
      301 tgcgttccgc cgcgtgctcc tggcctcgct tcccggttca gcggtttctt ccgtgaagat
      361 cgacggggtt ctgcatgaat tctgcagtat tcccggtgtg gtcgaagatg tgaccgagat
      421 catcctcaac ctcaagaagc tgatcgtaag cctgaaacag gatgaacccc tgaccatccg
      481 cctcgaagtc aagggtccct gccaggtgac cgctgctgac ctcggctcgc acccgacgct
      541 ccagatcgtt gatccgtccg tgcatctctg tcatgtgacg ggcgacatca acttcggcat
      601 ggacatcacg ttcaagcgtg gccgcggcta cgtgctggca gaaggccaca aggaaagcgg
      661 gcagcctctg ggtgtgattc tggtcgattc gcagttttct cccgtgacga aggtgaactt
      721 caacgtggaa gacgcccgcg tcggtcagga aatcaacttc gacaagctga cccttcaggt
      781 ctggacaaac ggcagcatga aaccgcacga atcggtcgag ctggccgcga agttcctgcg
      841 cacccacgtc gatctcttcg ccaacctgca ggaagatgaa gagtcggtcg acgaggaagt
      901 cgtgaccaag tctgaagatc tggtcatggt gtcgcagcag aagaaagaac ccacgcagaa
      961 gagctacact gacgttctca tcaaggacct cgagttctcg gtgcgctcac gcaactgtct
     1021 caagaaggcc ggcatccaca acctggctga cctggtcaac aaaaaggctt cagaactcct
     1081 cgagatcaag aacttcggca agaagtcgct caaggaagtt cgtgagaagc tggtacaatt
     1141 caatcttacc ctgcccggtg acgaaaacgg cggaggagac gtatgaaaca ccagaaaatc
     1201 ggacggaaat tcggacgtga gtcccatcag cgcgttgctc tgattcgctc tctgtgcacc
     1261 tctttggtga agtatgagcg catcgagacc acgttgcaca aagccaaaga cctgcgccgc
     1321 gtgatcgagc gcgccgtgac tatggcgaag aaagacaacg ctcagaagaa tcctgagctg
     1381 gcgaacttct tccatgccta cggcgataaa gaaatcatcg gccgcgacga catcaagcgc
     1441 tatctctcga acctcaagaa agaagtgcgc gagaaggtcg aaaagtatct cgccgacccg
     1501 gcaaaagccg agaaacccga gtttgtgctc gagtatctcg cttcgaccgg tgaccgcgcc
     1561 aagggtcctc gcatcctgcg cctggaaggc gtgctgacca aactggtcaa gcgcatcgct
     1621 ccccggttca aggataccaa cggtgggtat acccgcatct acaagctggg ttgccgccgc
     1681 ggcgacagcg cggagatggc ggtcatcgaa ttcaccaagt gaaatcgatg agatgatccg
     1741 cttcaaccgg gtcaccttcc ggtacggaaa ccatcaggga acctcctcga tcctcgagga
     1801 ggtttctttt tctctggcat ccg
//
LOCUS       DAORHD010001098         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83822_length_1821_cov_2.655153, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001098 DAORHD010000000
VERSION     DAORHD010001098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83822_length_1821_cov_2.655153"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1583)
                     /locus_tag="PKO06_19015"
     CDS             complement(60..1583)
                     /locus_tag="PKO06_19015"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV71804.1"
                     /translation="MNRHRSVLTRLALSLLLLSLATIAMAMQPLPPNPELKKVFDSIK
                     VPSADINTRVRYLWKNDEAWYTRWAMVESARKTIDTTYFIVENDVFGWSYLGLLRKKA
                     REGVKIRLMIDGRINRFTHKLGDSDELEELARFPNVEIKLYNSPAKALLPMLTDLKKM
                     FASNHDKIIIIDGKLTIIGGRNIGKDYFVGKGEHKIVYRDSDILMEGEHVARQCKVAF
                     DEEFGNLKNWLIKPDRFNFKDQTTKLDVAYRVMNRYLQGRGTWNPEQMGDLSDDLKKH
                     VKLFSDEIVKYKNLSSYAGHQIWRGERSYPVKILDQHSCVGVRNDVAANLIRLIDAAK
                     HEVLMQNSYVVLSDALTEALKRASARGVKIILHTNSGASTDAIFPQAFFMNDWMQLLK
                     DCPTMRLIVAKDPSQRLHSKCFVFDGQYTIVGTYNLDPLSDNVNSELVALVEGSAFGT
                     QVRNRIYTDFAPGLEYKIQVERDGKVTSLMGPEHHIDEKLLKKMNMLRKIQWLRPLI"
BASE COUNT          379 a          487 c          527 g          428 t
ORIGIN      
        1 gtcagcgatt gctggccggg gcggtcaact gtccgaggac aaacgagctc agagttcgat
       61 cagataagag gtctgagcca ctgaatcttg cggagcatgt tcattttctt cagcagcttc
      121 tcgtcgatgt gatgctcggg gcccatcaac gacgtgacct tgccgtcacg ctcgacctga
      181 atcttgtact cgaggccggg agcgaagtcg gtgtagatgc gattgcgtac ctgcgtgccg
      241 aaggctgatc cttcgaccag agcgacgagt tcggaattga cgttgtcgct cagcgggtcg
      301 aggttgtagg ttccgacgat agtgtactgt ccgtcgaaga caaagcactt gctgtgcaga
      361 cgctggctgg gatccttggc gacgatcagg cgcatcgtcg gacaatcttt cagcagctgc
      421 atccagtcgt tcatgaagaa cgcctgcggg aaaatggcgt cggttgacgc gccgctgttg
      481 gtgtgcagaa tgatcttcac cccccgagcg ctggcgcgct tgagcgcttc ggtcagcgca
      541 tccgaaagga caacgtacga gttctgcatg agcacttcgt gcttcgcggc atcgatcagg
      601 cgaatcaggt tcgccgcgac atcgttgcgc acgccgacgc aggaatgctg gtcgaggatt
      661 ttgaccgggt aggaacgttc gccgcgccag atctgatgac ctgcatagct gctgagattc
      721 ttgtatttga cgatctcgtc ggaaaacagc ttgacatgct tcttcagatc gtccgaaagg
      781 tcgcccatct gttcgggatt ccaggtgccg cggccctgca gataacggtt catcacccga
      841 taggcgacat cgagcttggt cgtctgatcc ttgaagttga aacgatcggg cttgatcagc
      901 cagtttttca ggttaccgaa ctcctcatca aaagccactt tgcactgtct ggcaacgtgt
      961 tcaccttcca tcagaatatc gctgtcgcga tagacgatct tgtgttcgcc tttcccgacg
     1021 aagtaatctt tgccgatatt gcgaccgccg atgatggtca gcttgccgtc gatgatgatg
     1081 atcttgtcat gattcgacgc aaacatcttc ttaaggtccg tgagcatggg cagaagggct
     1141 ttggccggcg aattgtacaa cttgatctcg acgttgggaa accgggccag ttcttcgagt
     1201 tcatcgctgt ccccaagctt gtgggtgaag cggttgatgc ggccgtcgat catgaggcga
     1261 atcttgacgc cttcgcgggc tttcttgcgc agcaggccaa ggtaggacca gccgaacaca
     1321 tcgttctcga cgatgaaata cgtggtgtcg atcgtcttgc gggcagattc gaccatggcc
     1381 cagcgggtat accaggcttc gtcgttcttc cagaggtagc gaacccgggt attgatgtcc
     1441 gcggacggaa ccttgatgct gtcgaagact ttcttcagct cagggttcgg gggaagcggc
     1501 tgcatggcca tggcgatcgt cgccagagaa agcagcagca gagagagagc gagacgcgtg
     1561 aggactgatc gatgtcgatt cataccttcc tccgtcactt ctaagtgtac tgccaagtct
     1621 attctcaccc tcgtcatgtg tcaacagaca ctcgcggtac gacggaggca tccacttctc
     1681 tctcgatagc caattttcat cgacatcact cagcgattcg ccgttacggt acagagtgcg
     1741 cccctcgccg gcgatttgtt gaacgatgac aggggaaaaa acaagccgga gctcgacggc
     1801 cccctgcatg atcacgatgg g
//
LOCUS       DAORHD010001099         1819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_83947_length_1819_cov_3.456349, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001099 DAORHD010000000
VERSION     DAORHD010001099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83947_length_1819_cov_3.456349"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKO06_19020"
     CDS             complement(<1..1108)
                     /locus_tag="PKO06_19020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNV71805.1"
                     /translation="MHTVSRVVLLSIGTEILFGEITDTNAPFLAGELTRRGAEVIRTI
                     AVPDDRNRLAHSIGQALLEADLVLTTGGLGPTDDDPTREAVCDVLGEQPEVDAKALEH
                     LEAFFQSRGRPMPASNQKQAWKVPSVDVLENPIGTAPGWFARVGSKAVVSMPGPPREM
                     KEMWAKQVLPRLTFGSPGFWEQTLHTCGIGESMAAERLGALTRSVNPYVGTYARRAGV
                     DIRVSARAGTSTEAQRLAAPVVDQVRLLMGDNLYGCDDDTLASAVGANLRARGQTIAT
                     LESVTGGLVADLLTDVSGSSAWVVGGAVGYTIAAKVRFGVSESLIREQGVVSEPVAQA
                     MAVAAKEWFRADWGVATTGVAGPDAHGGCPPGHTC"
     gene            1265..1780
                     /locus_tag="PKO06_19025"
                     /pseudo
     CDS             1265..1780
                     /locus_tag="PKO06_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
BASE COUNT          381 a          571 c          528 g          339 t
ORIGIN      
        1 tacacgtgtg ccccggaggg cagccgccgt gagcgtcggg accggcaact ccggttgtcg
       61 ccacacccca gtccgcgcga aaccactctt ttgcagcaac tgccattgcc tgagcgaccg
      121 gctcagagac gacgccctgt tcacgaatca gcgactcgga cactccgaat ctgaccttgg
      181 cagcaatcgt gtaaccgacg gcaccgccaa ccacccaggc actcgaacca gagacatcgg
      241 tcagcaaatc agccactaag ccgccggtga ccgactcgag ggtcgcgatg gtctgtccgc
      301 gagcgcgcag gttggcgcca acggctgagg cgagagtgtc gtcgtcgcaa ccgtacaggt
      361 tgtcacccat caacaacctc acctgatcga caacaggagc cgcaagtctc tgcgcctcgg
      421 tactcgtgcc ggcacgagcg gagacacgaa tgtcgacgcc tgctcgacgg gcataggttc
      481 ccacataggg gttcaccgag cgggtgagcg ctcccagacg ctcggccgcc atactttcgc
      541 caataccgca ggtatgaagc gtctgttccc agaaacccgg ggaaccgaac gtgagacgcg
      601 gcaaaacctg cttggcccac atctctttca tctcgcgcgg tggccccggc atactgacaa
      661 ccgctttgga tccaacgcga gcaaaccagc ccggtgcagt accaatcggg ttctcaagaa
      721 cgtcgacact cggaactttc caggcctgtt tttgattact ggccggcatg gggcggccgc
      781 gagactggaa aaacgcctcg aggtgttcga gtgctttcgc gtcgacttca ggctgttcac
      841 ccaacacgtc gcagacagct tcacgggtcg ggtcgtcgtc ggtcggccct aatccacccg
      901 tggtcagcac gagatcagcc tcaagcagcg cctgcccaat gctgtgcgcc agtcgattcc
      961 gatcgtcggg cacggcgatc gtacgaatta cttcggcacc gcgccgagtc agttcgccgg
     1021 ccagaaaagg cgcattcgtg tcggtgattt cgccgaagag aatttccgta ccaatgctga
     1081 gaagaacaac gcggctgacg gtatgcatat tcgcgttata cccgaaagac gtttcgtggt
     1141 ccagtgtaga aggggcagaa agaaggtggg aacctgttgc cgattctggc agtctgataa
     1201 gatgaacgct gaacattccc ctgggttgag ttacgtgttc ccgggtaccg aaaggactgc
     1261 tgatatgttg caaacaaccc tgctgctcgg tcttctgacc gccatgtttg tcgtcgccgg
     1321 aggcgctatc ggagggcagg aaggtatgac cattgctctt atcatggccg gggtcatgaa
     1381 tgttgtgagc tactggttct cagacaaaat cgtcctcagc atgtatggag cccgccaggt
     1441 cacggagcag gaagcgccgg atctggtcgg gatcgtccgt gaactgaccc aggctgccgg
     1501 aatgcccatg cccaaggtct acatcattcc atcggaatcg cccaacgcct tcgcgaccgg
     1561 ccgcaacccg gaaaacgcgg cggtcgccgc gaccgtcggc attctgcgtc tgctgccgcg
     1621 cgatgagttg cgcggtgtca tggcgcacga actggcacac gtgaacaacc gcgacattct
     1681 gatcagtacg gttgccgcga cactcgccgg ggcgatcacg tatctcgccc agatggccca
     1741 ctatgccgct ctgttcggcg gcagacaccg cgatgatgac acccgccaat gcgcctatcg
     1801 gtaacaggct gcccagccc
//
LOCUS       DAORHD010001100         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84113_length_1817_cov_3.047106, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001100 DAORHD010000000
VERSION     DAORHD010001100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84113_length_1817_cov_3.047106"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1056
                     /gene="whiG"
                     /locus_tag="PKO06_19030"
     CDS             247..1056
                     /gene="whiG"
                     /locus_tag="PKO06_19030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HNV71806.1"
                     /translation="MTAGTPPVHVPSEADTEDQKLWMQYKHTRDQRIKDTLIMKFASF
                     VKYVAGRIAVNLPSNVEFDDLVSYGILGLIDAIEKYDPERNVKFKTYAKTRIRGAIFD
                     ELRVLDWTPRSIRQKARKLEKAYAKLEGKLGRDATDDEVAEYLKIDISELHKLYDETK
                     KSLLLSLDEIFYDDEEGSSRFDFIENQKSDNPQRKIEEEEAKKLLADAIAKLSDRERL
                     VITLYYYEDLTSKEIGKILGVSDSRVSQLHTKAILRLRGRLSRMRDSLVTD"
     gene            complement(1166..1768)
                     /locus_tag="PKO06_19035"
     CDS             complement(1166..1768)
                     /locus_tag="PKO06_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV71807.1"
                     /translation="MTRKNYFISKGMQSKFAGTILLLVFLIAVISACNIYVLASFFMS
                     NQATISQEQTVEKMMSSFLVDFWPRLLLLVMVNVIIIFIVSIMYSHQIAGPAYKLEKS
                     LQRIAAGDLTFEIHLRRHDNLKEVASAINLMLAEFRETLASAKMLSEDVATKIKAFDK
                     DERLAPLAKSAAALQERIAKFKIEKTPEELEGAGSHANHG"
BASE COUNT          441 a          515 c          466 g          395 t
ORIGIN      
        1 tcggggacct gctgcaaccg ccgccgaacc tcgaccctta tgccgatgct cctcgccagg
       61 cggacgacgc actgaagccc gcagtgtttc cccgtttgac ggtagaaaac ggcaaagtcg
      121 tttcccgtgg cgactcgacc gccgtatcat aggcctcatt gagggaggga atcactcgtg
      181 accagagaaa gcatttctga aaaggcagag aaaccgacaa ccggcacccc cggtgcggaa
      241 ggctctctga cggcgggaac cccgccggta cacgttccca gcgaagctga taccgaagac
      301 cagaaactct ggatgcagta taagcatacg cgtgatcagc gcatcaaaga tacgttgatc
      361 atgaagtttg catcctttgt caaatacgtt gccggacgca tcgccgtgaa cctcccctcc
      421 aatgtggaat tcgacgatct ggtcagctac gggattctcg gcttgatcga cgccatcgaa
      481 aagtatgacc ccgagcgcaa cgtcaagttc aagacctacg ccaagacccg cattcgcggc
      541 gcgatttttg atgaactgcg ggttctggac tggacacccc gctcaattcg tcagaaagcc
      601 cgcaaactcg agaaagccta cgccaagctc gagggcaaac tcgggcgcga tgccaccgac
      661 gatgaggtcg cagagtatct caagatcgac atctccgagt tgcataagct gtacgatgaa
      721 accaagaaat cattgttgct gtccctcgat gagatcttct atgacgacga agaagggtct
      781 tcgcgattcg atttcatcga aaaccagaaa agcgataatc cccagcgcaa gatcgaagag
      841 gaagaagcca agaaactatt ggccgatgcc attgcgaagc tctcagaccg cgaaagactc
      901 gtcatcacct tgtactatta cgaagatctg acttcgaagg aaatcggcaa gatcctcggc
      961 gtttcggatt cccgcgtctc gcagctgcac acgaaagcta ttttgagact gcgcgggcga
     1021 ctgtcgcgca tgcgcgactc tctcgttact gactgattgt tcagtactat cccgtaaaac
     1081 acatcggccg ctctggttca ccagagcggc cgatgtgttt tacactttaa gggtacaccc
     1141 aactttgccg tgggtgctcc gtcagtcatc catggttcgc gtgactgcca gccccttcaa
     1201 gctcctcggg ggtcttttcg atcttgaact tggcgatgcg ttcctgcaga gccgccgcac
     1261 tttttgccaa cggtgcgagc cgttcatcct tgtcgaacgc cttgatcttg gtggcgacat
     1321 cttcactcag cattttcgcg ctcgccagag tttcgcgaaa ctcggccagc atgagattga
     1381 tcgctgaagc cacttctttc aggttgtcgt ggcggcgcag gtgaatctcg aaggtcaggt
     1441 cgccagcggc aattcgctgc agactcttct ccagtttata ggccggcccg gctatctgat
     1501 gcgagtacat gatgctgaca atgaaaatga tgatgacatt caccatgacc aacagcaaca
     1561 accgcggcca gaaatcgacc aggaagctgc tcatcatctt ctcgaccgtc tgttcctggc
     1621 tgatggtcgc ctggttcgac atgaagaaac tggccagcac atagatgttg caagccgaga
     1681 tgacggcaat cagaaacacc agcagcagaa tggttccggc gaacttgctc tgcatgccct
     1741 tcgaaatgaa atagttcttg cgcgtcaaac gtgaacctcc tctgagagga tgaatggact
     1801 tatcctatcg tatgacc
//
LOCUS       DAORHD010001101         1817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84135_length_1817_cov_2.395573, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001101 DAORHD010000000
VERSION     DAORHD010001101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84135_length_1817_cov_2.395573"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..>1817)
                     /locus_tag="PKO06_19040"
     CDS             complement(62..>1817)
                     /locus_tag="PKO06_19040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNV71808.1"
                     /translation="AGEHWTPRDAVKLMADLIFLPIADRLESGSYLLYDGACGTGGML
                     TVAEERLFELGKARGKKIVPHLYGQEINPETFAICKADMLLKGEGENAEHIRGGAEWS
                     TLSHDAFPAQTFDFMLSNPPYGKSWKKDLEHMGGKDGMRDPRFKVMHREVELSLVPRS
                     SDGQMLFLANMAHKMSTKTALGSRIAEVHNGSSLFTGDAGQGESNIRRWLIENDWLEA
                     IVALPLNMFYNTGIATYIWVLCNRKPEHRQGKVQLIDATQWFKPLRKNLGKKNCELSE
                     EDIQRICDVYLAFKETEQSKIFPNEGLGYWKVTLERPLRLHSRLSDEAIDGLRFASGD
                     EELRRELFAEFGQALYIDFATGKPQVDAFLEAKGAGGDEESDEGAENKGLPEKVVKRL
                     LDPKTWAHDGQLAAAAQVVQKALGKRLFEDHNEFRALVNDALDQERIKLSGPDKKALL
                     KAVSWREESAPPVIARVHKPGKVRPDPLRGLYQVTIDGKPAIAEYETDTDLRDTEQIP
                     LLEDGGIEAFLKREVLPYTPDAWLDDSKTQIGYEISFTRHFYKPQPLRTLDEIRADIR
                     ALEQETDGLIAEIIGTSK"
BASE COUNT          318 a          537 c          536 g          426 t
ORIGIN      
        1 ggcgagacca aagtgaactg aatgttgctg agcggagaag cagagaggta atgaaacatt
       61 ctcatttgct tgttccgatg atttcggcga tgaggccgtc ggtttcctgt tcgagggcgc
      121 ggatgtcggc gcggatctcg tcgagcgtgc gcaggggttg gggcttgtaa aagtgtctgg
      181 tgaagctgat ctcgtagccg atctgggttt tgctgtcgtc gagccaggcg tcgggcgtgt
      241 agggcagcac ctcgcgtttg agaaacgcct cgataccacc atcttcgagc agcggaatct
      301 gctcggtgtc gcgcaggtcg gtgtcggtct catactcagc aatggcgggc tttccgtcga
      361 tggtcacctg gtacaatccg cgcaacgggt cgggcctcac ctttcccggt ttgtgcacgc
      421 gcgctatcac tggcggcgct gattcctctc gccagctgac ggctttgagc agagcttttt
      481 tgtcaggccc tgacaacttg atgcgctcct ggtccagcgc atcgttcacc agcgcgcgaa
      541 actcgttgtg atcctcgaac aaccgcttgc caagagcttt ctgaacaacc tgagccgcgg
      601 cagccagctg tccgtcatgc gcccaggttt tcgggtcgag cagtctcttc acgaccttct
      661 cgggcagtcc tttgttctcg gccccctcgt cgctttcctc gtctccgcct gcccccttcg
      721 cttcgagaaa ggcgtctacc tgcggtttcc cggtggcgaa atcaatatac agagcttgcc
      781 cgaactcggc gaacagctcg cggcgcagct cctcgtcgcc cgaggcaaag cggaggccgt
      841 cgatggcttc atctgacaga cgactgtgca ggcgcagagg gcgttccagc gtgactttcc
      901 agtatccgag cccctcgttc gggaaaatct tcgactgctc ggtctctttg aaggccagat
      961 agacgtcgca gatgcgctgg atgtcttcct cagacaactc gcagttcttc ttgccgaggt
     1021 tctttcgaag cggcttgaac cactgagtcg cgtcgatgag ttgtactttc ccctgtcgat
     1081 gttccggctt gcggttgcag agaacccaga tataggtggc aatgccggtg ttgtagaaca
     1141 tgttcagcgg cagggcgacg atggcttcga gccagtcgtt ttcgatgagc cagcggcgaa
     1201 tgttgctctc gccctgcccg gcatcgccgg tgaatagaga cgagccgttg tgtacctcgg
     1261 cgatgcggct gccgagcgcc gtcttggtgc tcattttgtg cgccatgttc gcgagaaaca
     1321 gcatctggcc gtcgctcgaa cgcggcacca gtgacaactc gacttcgcga tgcatcacct
     1381 tgaatcgcgg gtcgcgcatg ccatctttgc cacccatgtg ttcgagatcc ttcttccaac
     1441 tcttgccgta agggggattc gagagcatga aatcaaaggt ctgcgcgggg aacgcatcat
     1501 gtgacagcgt gctccactca gcgccgcccc tgatgtgctc ggcgttctcg ccttcgcctt
     1561 tcaacagcat gtcggcttta cagatggcga aggtctcggg gttgatctcc tgcccgtaga
     1621 ggtgggggac gatcttcttg ccccgggctt ttcccagttc aaagagcctt tcttctgcaa
     1681 ccgtcaacat gccgcccgta ccgcaagcgc cgtcatagag cagatacgag cccgattcga
     1741 gccggtcggc gatcggcaaa aagatcagat cggccatcag tttcaccgcg tcgcgtgggg
     1801 tccagtgttc gcccgcc
//
LOCUS       DAORHD010001102         1816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84163_length_1816_cov_3.866553, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001102 DAORHD010000000
VERSION     DAORHD010001102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84163_length_1816_cov_3.866553"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..541
                     /locus_tag="PKO06_19045"
     CDS             134..541
                     /locus_tag="PKO06_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71809.1"
                     /translation="MTVLEVLLFCSYINLLAMLPLFSSRRLAEHMSGNLDQWYSWFIS
                     SPGWMALAVILATSGIAWKLTGTGEQQSTYQRAIAVNSLLMIVCWVLPSACGASDWLQ
                     MVGMEIWAIASGLFLLFASFLVPVKALRRALKG"
     gene            600..>1816
                     /locus_tag="PKO06_19050"
     CDS             600..>1816
                     /locus_tag="PKO06_19050"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71810.1"
                     /translation="MTDDLFTTSVEKLKTGTEEERKEAVLALFKAKDRRAIDVFAKIC
                     EMDRSSEVRTQARKAYYLLRDIHPNNLSEPFLELPDGISFDDLEKLLVDENPRIRGEA
                     IKLCSKLDPASTAPPLHIAIACEKNARLQAGILRGLGRAGLKYDIPTIAEFLTKGDSD
                     LQIAAIEGLAMIGGADALEFVVPYLRDPDSSVRAAATKALPPLEGGEMLNILRGMAIS
                     TNQAWREAVIHQIQRFKVPLASKILAHIAAVDPVIALRDRARAGLEAMARSDENARKE
                     LEELNTPEEPEDPNAPATVPLDGIWETAPDTPLPAQGTPGPAAGASTPDSATPPTSQT
                     TPAGTAAAASSGVGAAPAKPRVLVVPGVTKKLVKSICLGDQAQRQDGLRQLAGLLKPE
                     HLPFLLLQLDREK"
BASE COUNT          369 a          557 c          542 g          348 t
ORIGIN      
        1 gagatcccgc aaggtcgggg aacgcggtcg acagtgggtt gagaaaaaga tcaatcccga
       61 gcgcgaggcc gcggcctatg ctgaaatgta ccggcagctg gtatgagaat gcagtgtctg
      121 aagcgataaa ccgatgaccg ttctcgaggt tctgctgttc tgttcataca tcaacctgct
      181 ggccatgctg ccccttttca gttcgcgccg gttggccgaa cacatgtcgg gcaatctcga
      241 ccagtggtac agctggttca tctcttctcc cggctggatg gcactggccg taatccttgc
      301 caccagcgga atcgcctgga agctgaccgg taccggcgaa cagcaatcga cgtatcaacg
      361 ggccattgcc gtgaattcgc tgctcatgat cgtctgctgg gtcctgcctt ctgcctgcgg
      421 agcttcggac tggctgcaga tggttggtat ggaaatctgg gccatcgcat caggcctgtt
      481 tctgctcttc gcgagttttc tcgttccggt caaggccttg cggcgcgccc tgaaaggttg
      541 agggaaccgg gcgaaaggtg taagataacc gtctgaaacc cgtgaccgag ggggtgttcg
      601 tgaccgacga tctgtttacc acatccgtcg agaagctcaa gacggggacg gaagaagagc
      661 gaaaggaagc ggttctcgct ctgttcaagg cgaaggatcg gcgcgcgatc gatgtgttcg
      721 cgaagatctg cgaaatggat cggtcgtccg aagtgcgaac tcaagcgcgc aaagcctact
      781 acctgctgcg cgacattcac cccaacaatc tctccgagcc ttttctcgag cttccggacg
      841 ggatcagttt cgatgacctc gaaaaactgc tggtcgacga gaacccgcgc attcgcggcg
      901 aagcaatcaa gttgtgcagc aagctggacc ccgcctcgac cgcaccaccg ctgcacatcg
      961 cgattgcctg cgaaaagaac gcgcgtttgc aggccggtat tctgcgcggc ctgggtcgtg
     1021 ccggtctgaa atacgacatt ccaaccatcg ccgagttcct gaccaaaggc gattccgatt
     1081 tgcagatcgc ggcgatcgaa ggcttggcta tgattggcgg tgcagacgcg cttgaattcg
     1141 tggttcccta cttgcgcgac ccggacagtt cagttcgcgc tgcggcgacg aaggctctcc
     1201 cccctctcga gggcggcgag atgctcaaca tccttcgcgg gatggcgatc tcgaccaacc
     1261 aggcctggcg tgaagcggtc atccatcaga ttcaacggtt caaggtgccg ctggcctcga
     1321 agattctggc acacatcgct gccgttgatc cggtgatcgc tctgcgtgat cgtgcgcgcg
     1381 ccggtctcga agccatggcc cgcagcgatg agaatgcgcg taaagaactc gaagaactga
     1441 acacgcccga agaacccgaa gacccgaacg ccccggccac ggttccgctc gacggcatct
     1501 gggaaaccgc tcccgacaca ccactacccg ctcaaggcac tcccgggccg gcggccggcg
     1561 cgtcgactcc tgacagcgcc accccgccca cgagtcagac aacacccgcc ggaacagccg
     1621 ccgcggccag ttcaggtgtg ggtgcggccc ccgcgaaacc tcgggtgctg gtggttcccg
     1681 gggtcaccaa gaagctggtc aagtcgatct gtctcggaga tcaggcccag cgccaggatg
     1741 gcctgcgcca gttggccggt ctgctcaagc ccgaacatct gccgttcctg ctgctgcaac
     1801 tcgaccgcga aaaaga
//
LOCUS       DAORHD010001103         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84321_length_1814_cov_4.465605, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001103 DAORHD010000000
VERSION     DAORHD010001103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84321_length_1814_cov_4.465605"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKO06_19055"
     CDS             <1..546
                     /locus_tag="PKO06_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV71811.1"
                     /translation="ARKSGITLLEIMFAVIILATAMIPIASMIGFGFRGTMKDYRNIV
                     ALQLLESTLNQVQSAAYDTIPEGTHTTTLSLVATDTNARIPLGTIASGGYTFNVSLNV
                     TGENVTFSYQSVQYNLAGFDEDNPATWQFSGVQTVSYNQASGLFARRVRRVVAIVDWT
                     EPNAAPRTVRAVTFKVRMRQS"
     gene            543..1052
                     /locus_tag="PKO06_19060"
     CDS             543..1052
                     /locus_tag="PKO06_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71812.1"
                     /translation="MSHHQQLRKRGTALVIVLVFATVLLTFGLFYLRTVSQSVPINPL
                     QLRRIQADFLAEGLANIAMLKYQELPSMFHYSYIAGRIATNGANIGPLNTYLGDSYLN
                     GNSTINTALADPNAPALPITFTTDYRILSHKKYDRDSIQIICTVTMGSVMEREVRRTF
                     YAERRRSVP"
     gene            1049..>1814
                     /locus_tag="PKO06_19065"
     CDS             1049..>1814
                     /locus_tag="PKO06_19065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV71813.1"
                     /translation="MKRRGMTLTEIIIAAAIFSGFMVAVFSIYRSGTESFITGSWRVN
                     AQKQARAFLTQLRDDLESANNPAVMATDTTTFPASTPIFLNAGGFADHASTTQPLLSL
                     SGTTWTPACFMVISRPCQRGTIFQPAAQGQWVGVSVWGQSRKLRYVRSGVWGTFSGSP
                     AAFPTGVTYSAFQTACVTSGDFAAATQKALNQTFLEDADRISFRRKWRTLGGQQEMVL
                     EIVLRMVRMRNGIPLNDTFVEERIDAKIQPGTLVNTF"
BASE COUNT          361 a          582 c          488 g          383 t
ORIGIN      
        1 gcacgcaaga gtggaatcac ccttctcgag atcatgttcg cggtgatcat tctggcaacc
       61 gcgatgattc cgatcgcctc gatgatcggc ttcgggttcc ggggtacgat gaaggattac
      121 cggaacatcg tagcgctgca actgctcgag agcacgctca atcaggttca gtcagcagcc
      181 tatgacacca ttcccgaagg cacgcataca acaacgcttt ctctcgtggc aaccgacacg
      241 aatgcccgca ttccgctcgg gaccatcgct tcaggcggct atacgttcaa tgtttcactg
      301 aacgtcaccg gtgagaatgt cacgttttca tatcaatcgg tgcagtacaa cctggccggg
      361 ttcgatgaag acaatcccgc cacctggcag ttttcaggag ttcagacggt gagctacaac
      421 caggcgagcg gcctgtttgc ccgtcgggtg cgacgcgtgg tggcaatcgt ggactggacg
      481 gagccaaacg ccgcgccccg caccgttcgc gcggtcacgt tcaaggtcag gatgcgacag
      541 tcatgagtca ccaccaacag ctccgcaagc gcggcaccgc gctggtcatc gtgctggtct
      601 tcgccacggt gctgcttacc ttcggcctgt tctatctgcg gacagtttct cagagcgtac
      661 caatcaatcc tttgcagttg cgccgcattc aagctgattt tctggccgag ggattggcga
      721 atatcgccat gctcaagtat caggagcttc cctcgatgtt ccactactcc tacattgcgg
      781 gccgcatcgc cacgaacgga gccaacattg gtccgctcaa cacgtatctc ggcgattcgt
      841 atctgaacgg caactcgacg atcaacaccg cgctggctga cccgaatgcg ccggcactgc
      901 cgattacctt tacgaccgat taccgcattc tgtctcacaa gaaatacgac cgcgactcga
      961 tccagatcat ctgcacggtc acgatgggtt ccgtgatgga gcgtgaagtc cggcgaacct
     1021 tttacgccga gcggcgcagg agcgtgccat gaaacgacgc ggcatgaccc tcacggaaat
     1081 catcattgcg gccgcgatct tttcgggctt catggttgcc gtgttctcga tctaccgatc
     1141 gggcaccgag agcttcatca ccggctcgtg gcgtgtgaac gcgcagaagc aggcccgggc
     1201 ctttctcacc cagttgcgcg acgatctcga atcagccaac aatccggccg tcatggccac
     1261 cgacaccacc acgtttccgg cctcaacgcc gatttttctc aacgccggcg gctttgccga
     1321 tcacgcgtcg acgactcaac ccctgttgag cctttccggc accacctgga ctccggcctg
     1381 tttcatggtc atctcccgcc cgtgccagcg tggcacgatc tttcagccag cggctcaggg
     1441 gcagtgggtc ggcgtcagcg tctggggtca gagtcgcaaa ttgcgctacg tgcgtagcgg
     1501 ggtctggggc accttttccg gctctccggc cgcttttcca accggcgtga cctactccgc
     1561 ctttcagacc gcctgtgtca cgtcgggcga tttcgcggcc gccacccaga aagctctgaa
     1621 tcagacgttt ctcgaagacg ccgatcgtat ctcgtttcgt cgcaaatggc gaaccctcgg
     1681 cggccagcag gagatggtac tcgagatcgt gctgcgaatg gtccgcatgc gcaacggcat
     1741 tcccctcaac gacaccttcg tcgaagagcg catcgacgcc aaaattcaac ccggaaccct
     1801 cgtgaacacc ttct
//
LOCUS       DAORHD010001104         1814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84360_length_1814_cov_2.580443, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001104 DAORHD010000000
VERSION     DAORHD010001104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84360_length_1814_cov_2.580443"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKO06_19070"
     CDS             <1..358
                     /locus_tag="PKO06_19070"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HNV71814.1"
                     /translation="ELLGYVSELAMTAGALDVALTPILMKKGRPGTRFSVLCRPGEAE
                     TLETLLYRELPTLGIRRQLIQRSTLQREECRQETAAGSLAAKRIQEWDGSVSETLEFE
                     SRKLLAAHTGKPVRKL"
     gene            406..1491
                     /locus_tag="PKO06_19075"
     CDS             406..1491
                     /locus_tag="PKO06_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HNV71815.1"
                     /translation="MTKKNSTNTRSRDLLKKLTLAHGAPGFEGPVAQVIEHELKSFGT
                     ISRDRLGSILVEKTGKSGGPRIMLAAHMDEVAFMVKAIPESGLIKFVPLGGWWPHVLP
                     SQRVSILTPKGEVPGVIGAAPPHHLSPADREKVMAIKSMVIDIGATSAKEARAMGVEL
                     GQAIVPAVTFQEMANPEIISSKAFDDRVGCALMIEVMHRLGKNHPNVVIGAGTAQEEV
                     GVRGAKTAVTLANPDVAIVLEGPPADDLPGAADFVQCGMGKGPQIRAYDPTMVTNPRL
                     VDLFRTRAEALKLPFQMAVRDSGGTDASVIHVYSRGVPSMVIGVPVRYAHSHLGLMHL
                     QDFENTVKLVIDVIQHLDAKTVQALHG"
     gene            1611..>1814
                     /locus_tag="PKO06_19080"
     CDS             1611..>1814
                     /locus_tag="PKO06_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71816.1"
                     /translation="MFRNRFRIRLLSLATVLVFTLSTFGAYAQEVITGPQAQPPFNWA
                     ALLGASPEVGVLNSNLHTVENSLK"
BASE COUNT          388 a          530 c          548 g          348 t
ORIGIN      
        1 cgaactgctg gggtatgtct cagaactcgc gatgaccgcc ggagcgctcg atgttgctct
       61 gaccccgata ttgatgaaaa aggggcgacc ggggacccgt ttctcggtgt tgtgtcgacc
      121 aggcgaggcg gaaactctgg aaacgctgtt gtatcgcgag ctgccgacgc tcggcatcag
      181 aaggcagttg attcagcgtt cgacgctgca acgcgaagaa tgccgtcagg aaacggcggc
      241 cggctctctg gcggccaaac gcattcagga atgggatggc agcgtgagcg agacgctcga
      301 attcgagtct cgaaaactcc tggcggcgca caccggaaaa ccggtccgca agctctaatt
      361 cacgattctc tacccagaca tcacggaaag gacacgtcac aaaccatgac caagaagaat
      421 tccaccaaca cccgctctcg cgacctgttg aagaaattga cactggcgca cggcgctccc
      481 gggttcgaag gtccggtggc tcaggtgatc gagcatgaac tcaagagctt cggcacgatc
      541 agtcgcgacc ggctgggcag catcctcgtc gagaaaaccg gaaaatccgg cggaccgcgt
      601 atcatgttgg ccgcgcacat ggacgaagtc gcgttcatgg tcaaagccat tccggaatcg
      661 ggactgatca agttcgtgcc gctcggaggc tggtggccgc atgtgctgcc ttctcaacgc
      721 gtgtcgattc tgacgccgaa gggcgaggtt cccggcgtca ttggtgccgc accgccgcat
      781 catctgagcc ccgccgatcg cgagaaagtc atggcgatca agagcatggt gatcgacatc
      841 ggtgccacga gcgccaaaga agctcgcgca atgggcgtcg aactgggtca ggccattgtt
      901 cctgcggtca cgtttcagga gatggccaac ccggagatca tttcgtcgaa ggcgttcgac
      961 gaccgtgtcg gctgcgcgct gatgatcgag gtcatgcatc ggctcgggaa gaatcacccg
     1021 aacgtggtga tcggtgcggg cacggcccag gaagaagtgg gcgtacgggg cgcgaaaaca
     1081 gccgtcacgc tggcaaatcc tgatgtagcc atcgtcctcg aaggtcctcc ggcggacgac
     1141 ctgcccggtg ccgccgattt cgtgcaatgc ggcatgggaa aagggcctca gatcagagcc
     1201 tatgacccga ccatggtcac caatccgagg ctggtcgact tgttccgcac gcgcgccgag
     1261 gccttgaaac tgcctttcca gatggccgtc cgcgacagcg gcggcacgga tgcctcggtg
     1321 attcacgtgt attcacgcgg agttccgtcg atggtcatcg gggtgccggt gcggtatgcc
     1381 cacagtcatc tgggtctgat gcatctgcag gacttcgaga acaccgtcaa actggtgatc
     1441 gacgtcattc aacacctgga tgccaaaacg gtacaagccc ttcacggtta aaccggcgat
     1501 gcggtgggcg tcgaaacatt gacgcccccc ggcatcattc ttataattga agaaaatctc
     1561 tgcgtttcgg gcttgatgac gggtttcaaa cgcaaacgga ggtactgacg atgtttcgca
     1621 atcgattccg cattcgtctg ctctcactgg ccacggttct ggtgttcacg ctctcgacct
     1681 tcggcgccta cgcccaggag gtcattaccg gtccccaggc acaaccgcca ttcaactggg
     1741 cagccctgct gggtgcgtct cccgaagtcg gcgtcctgaa cagcaatctg catacggtcg
     1801 agaactcgct gaag
//
LOCUS       DAORHD010001105         1813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84421_length_1813_cov_2.890216, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001105 DAORHD010000000
VERSION     DAORHD010001105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84421_length_1813_cov_2.890216"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKO06_19085"
     CDS             <1..427
                     /locus_tag="PKO06_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71817.1"
                     /translation="IDMTGNTPHSEKLQVPADRMSGTSKTAVLKDVASHEIVLQPVTD
                     EPETATPCGTDLTACSLEIAREPVVETGSLNEAHDPIDESKKTVPSWRVTKPMQLGQI
                     PAWLQGPDSASAKPTRTRREHKRKTLLEMFSTPLWEDNL"
     gene            430..>1813
                     /locus_tag="PKO06_19090"
     CDS             430..>1813
                     /locus_tag="PKO06_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71818.1"
                     /translation="MSHRTRVNGATLLLLGCLMLIEVALQAQPAPPLQAQVYSYLTFN
                     RIRWWHPSPSAGVTSYLIYRSTSPDASVSGAQIASVAATLGVTPQVYDDNTAVYAQEY
                     YYTVIAVAGGLNSGPSEEVKRFLPRWGYGRIPQQVSHRRAYQYLNWDPYPGQWNHVYL
                     RFDTNPNVTAAVTPGQVVNNNHAVRWSRTDTLTNGTNYYLRLAMGRPDWVSNPNAVFY
                     TSYSTSLPLLVDDNDTFADLASSTYFNNSLSYVGCFPAPAGGVQLQTFTGSGGGFWRY
                     RQGLSVRERGITSRVNAPVRHTVSAIPVTQPLAAGRSAIEVRVSDEFGNEMPCWVASE
                     TTSAGLVTAFTVHFLAEGIWNNDTRYYWVYWGNPAAVSPGFAWAQNATRTSQWDVTPW
                     FSRKVLKAGLDTYTSTNGNRVIPSPPNPADDDNALFTLPWGASWFYELSTSTWYVSAN
                     GYMSTASYSTG"
BASE COUNT          399 a          496 c          522 g          396 t
ORIGIN      
        1 aatcgacatg acaggcaata ccccgcactc cgagaagttg caggttcctg ccgaccgaat
       61 gagtggcacc tcgaaaacgg cagtcttaaa agatgttgca tcacatgaga tagtactcca
      121 gccggtcaca gacgaaccag aaacggcgac gccttgtggc accgatctca ccgcgtgctc
      181 gctcgaaatc gccagagagc cagtggtcga aaccggaagt ctgaatgaag cacacgatcc
      241 aatcgacgag agcaagaaga ccgtgccatc gtggcgtgtc accaagccca tgcagctggg
      301 gcagataccg gcatggctgc aggggcctga tagcgcttcg gcgaagccaa cgcgaacgcg
      361 tcgggaacac aagcggaaga cgcttctcga gatgttcagt acgcctctct gggaggacaa
      421 cctctaaaca tgagccatcg tacacgggtg aatggcgcga cacttcttct gcttgggtgt
      481 ctgatgctga tcgaggtggc gttgcaggca cagcccgctc cgccgttgca ggcgcaggtg
      541 tattcgtatt tgacgtttaa tcgcattcgt tggtggcacc cgtcaccctc ggcaggtgtg
      601 accagttatc tcatctatcg cagtaccagc ccggatgcca gtgtcagcgg tgcgcagatt
      661 gcttcggtgg ccgcgacact gggggtgacc ccgcaggtgt atgacgacaa caccgctgtc
      721 tatgcgcagg aatactatta caccgtcatc gctgttgctg gcggtttgaa ttcaggacca
      781 tcggaggagg tgaagcgatt cctgcctcga tggggatacg gccgtattcc gcaacaggtc
      841 agtcatcgtc gggcatacca atacctgaac tgggacccgt acccgggcca gtggaatcat
      901 gtgtatcttc ggttcgacac caacccgaat gtcactgcgg ctgtaacccc tggccaggtg
      961 gtcaataaca atcatgcggt tcgatggagt agaacagata cgctgaccaa tggaaccaat
     1021 tattatctgc ggttggcgat gggtcgtccc gattgggttt ccaatccgaa cgccgttttt
     1081 tacacgtcct acagcacgtc gttaccgctt cttgtcgatg acaacgatac ctttgccgac
     1141 ttagcgtcgt cgacgtactt caacaatagt ttgagctacg tcgggtgttt tcccgcacct
     1201 gccggaggag tccaactgca gacgttcacc ggttcgggcg gcggattctg gcgttaccgg
     1261 caaggactgt ccgtaagaga gcgtggcatt acttctcgag tgaatgcacc cgttcgtcat
     1321 acggtgtcgg caatcccggt cactcagcca cttgcggcag gtcgcagcgc gatcgaagtg
     1381 cgagtatcag acgagttcgg caatgaaatg ccttgctggg tggcgagtga aaccacttcg
     1441 gcagggttgg taacggcgtt tacggtgcac tttctcgccg aagggatctg gaacaatgat
     1501 acacgctact attgggtcta ttggggaaat ccggcagcgg tgagccctgg gtttgcctgg
     1561 gcacagaacg ccactcgaac ctcgcagtgg gacgtcactc cctggttctc gcgcaaggtg
     1621 ctaaaagctg gtctggatac gtataccagc acgaacggca atcgggtgat tccttcgcct
     1681 ccgaacccgg cagatgatga caatgctctg ttcacgttgc cctggggtgc atcgtggttt
     1741 tatgagctat cgacgtcgac ctggtatgta tcagccaacg gctacatgtc gacagcatcc
     1801 tattcaacgg gtt
//
LOCUS       DAORHD010001106         1812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84505_length_1812_cov_2.524758, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001106 DAORHD010000000
VERSION     DAORHD010001106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84505_length_1812_cov_2.524758"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1812)
                     /locus_tag="PKO06_19095"
     CDS             complement(<1..>1812)
                     /locus_tag="PKO06_19095"
                     /inference="COORDINATES: protein motif:HMM:NF023676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin TcdB middle/C-terminal domain-containing
                     protein"
                     /protein_id="HNV71819.1"
                     /translation="LFIDRERILSHYRDEYFPRWFEDLRPNETNVLGTFRENILPDSD
                     IEAADLTGKEWREAMRACKGMPLRQEIYELDVDELEKGGQIPVKLFSTAYHNCHIHRL
                     QPRTENPHAVFHVTESEAITYQYELDLRPAEVTPDPRVLHNLNLTIDELGNVLQVVTV
                     VYPRRLSHDDETLSVGDRAAIDRVQKERHVTYTETRFTNDFGEKPEDTLLARDHYRLR
                     QPCEVTTYELKGLNPETDDVYFNLDELRAYRLSERYQSSSDMVEPLPYHHMWKEGDPR
                     SKRLVEQVRTLYFDANLTDPLRFGDLNHQGIVYEQYKLALTDDLLNAILADKLTPEVK
                     GAVANKQISGHLSGTTLVGWFQGENTAGQYWICSGTAGFESDAPQHFYLPECYTDPFG
                     NVTTLVYDDRDLFIESSRDAKGNVTQVDDFDFRVLAPFRMKDVNENLSEVRFDVLGTP
                     TALALMGKGTEADNLNGFDDALTNPDATLVRNFFTGDFNESTARQLLGNATARHLYYF
                     GETIKDGKLIWGSHPACAAGILRERHVASLQAGEVSSIQAAFEYSDGMGTVLVKKGQA
                     EPKIGETKLQWIASGKTILNNKGKPVKQYEPYFSLTEH"
BASE COUNT          373 a          490 c          485 g          464 t
ORIGIN      
        1 atgctcagtt aggctgaagt agggctcgta ttgtttgacg ggtttccctt tattgttcag
       61 gatggttttt ccgctcgcga tccattgcag cttggtctcc cctatcttcg gttccgcctg
      121 ccctttcttc accagcaccg tgcccatgcc atcggaatat tcaaaggccg cctgaatcga
      181 gctgacttcg cctgcttgca atgatgcgac atgccgttcg cgaagaatcc cagcggcaca
      241 ggctggatgg cttccccaaa tgagcttgcc atccttgatg gtttcaccga agtagtacag
      301 atgtcgcgct gtcgcattgc caagcagttg acgcgcggtg ctttcgttga agtcccccgt
      361 gaagaagttt ctgacgaggg tggcatcagg attcgtgagt gcgtcgtcga atccattgag
      421 attatccgcc tccgtgccct ttcccatgag ggccagcgcc gtcggcgttc ccagcacgtc
      481 gaagcgaact tcagacagat tttcattgac atctttcatc ctgaacggcg ccagcacccg
      541 gaagtcaaaa tcatcaacct gagtgacatt tcccttcgca tccctgctgg actcgatgaa
      601 caaatcccgg tcgtcataga ccaaggtggt tacgttgccg aaaggatctg tatagcattc
      661 cggcaaatag aagtgttgtg gagcatcaga ctcaaaccct gctgtccctg aacagatcca
      721 atattgcccg gctgtgttct ccccttgaaa ccaaccaacc agcgtggtcc cactgagatg
      781 gccgctgatc tgtttattgg cgactgcgcc cttcacctcc ggtgtcagct tgtcggcaag
      841 aatcgcgttc aagaggtcat ctgtgagagc cagcttgtac tgttcgtaca cgattccctg
      901 gtgattcagg tcaccgaatc gcaagggatc ggtgagattg gcgtcgaagt acagggtcct
      961 gacctgctcc acaagccgtt tactacgagg gtcgccctcc ttccacatat gatggtatgg
     1021 cagaggctca accatgtcgc tggacgattg atagcgctca ctcaagcgat aggcgcgcag
     1081 ttcatcgaga ttaaagtaca cgtcatcagt ttctggattg agtcccttca gttcataggt
     1141 ggtcacctcg cagggctgac gaaggcggta gtggtcgcgg gcaaggagtg tgtcctctgg
     1201 cttctctccg aaatcatttg taaagcgggt ttccgtatag gtgacatgtc gttctttttg
     1261 gactcggtcg atggccgcgc gatcgcccac ggataacgtc tcatcatcat gactgagccg
     1321 ccgtggatag acgaccgtga ccacctgcaa gacattgccg agttcgtcga tggtgaggtt
     1381 caggttatga agaacccgag gatccggtgt gacctctgcc ggtcgaaggt ctagttcgta
     1441 ctggtacgtg atggcttcac tttccgtgac atgaaagacc gcatgtggat tctcagtccg
     1501 tggttgcagc cgatgaatgt ggcaattgtg gtacgccgtc gagaagagct tgactgggat
     1561 ctgcccgccc ttttcgagct catcgacgtc aagctcataa atttcctgcc gcagcggcat
     1621 gcctttgcac gcccgcatgg cttcccgcca ctctttaccc gtgagatctg ctgcctcgat
     1681 gtctgaatct ggtaggatat tttctcggaa tgtgcccagc acattggttt cgtttggtcg
     1741 caggtcttcg aaccaacgag ggaaatattc atcgcgatag tgggagagaa tccgttcccg
     1801 atcgatgaag ag
//
LOCUS       DAORHD010001107         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84547_length_1811_cov_3.454442, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001107 DAORHD010000000
VERSION     DAORHD010001107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84547_length_1811_cov_3.454442"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1794)
                     /locus_tag="PKO06_19100"
     CDS             complement(<1..1794)
                     /locus_tag="PKO06_19100"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71820.1"
                     /translation="MHVKQQTTLRQEDYYLVLHALLDQDSEIRMTARMASSPYAKEPY
                     FVDPKGLSLDELTKKVEERFPGFTTDRPQMVEYKSETGYPQMSKELSNRQGRAERDGE
                     WDGPFPKAIGLLNALREDTQGMILNSLTQNETVDRAYIGFYIDAFKPFRDGKRSLDSH
                     TTVAIVNLNHATPVLEQYGEIETMIKQLERPVYLLVVLTNRRLILFLRDHVQSSRAVI
                     RPVSFGQMSRITSIQAQQTMALDIETHHDQVKIPPLLLADGLELDKLLKERSINAIQA
                     DESFIDRDFDKEISRLDLLYKARTIPSSEYMFRKMRLQKMELEKFSDKNIELLLAKRF
                     SDGTAGKKLDEQLLKKFTFEKTIMFTDIVGYSAKAAEKELLDTMTMLAVHDRTLMPII
                     AKHQGQLIKKIGDALMVKYDDPLEACYAAQEMQQALIEFNRSSDEKILIRIGINTGTV
                     FMKNNDVFGDAVNVAARMESMAQPGRIFITQGTEQKVREKISVQDCGSRRVKGQKHPL
                     RVFAIIDVTNADQEMLASAEAMKASLGLPSETESAVEESVDHVVSSSEAASTTSEPVR
                     SEPQTPEEAIEAMKSSIADAVQLYKKAVHLGY"
BASE COUNT          393 a          515 c          432 g          471 t
ORIGIN      
        1 atacccgaga tgaactgcct tcttgtacaa ctgcaccgcg tcagcaatcg aactcttcat
       61 cgcctcgatc gcttcttcag gagtctgcgg ttctgagcgt actggttcgc tggtcgtaga
      121 agcagcttcc gaagatgata ctacgtgatc aacactttct tcgaccgcgc tctctgtttc
      181 tgacggcaaa cccagactcg ccttcatcgc ttcggcactg gcaagcattt cctgatcagc
      241 attcgtaaca tcgatgatcg cgaacacccg caacggatgt ttctgcccct tcactctccg
      301 ggagccacaa tcctgcaccg agatcttctc acgcactttt tgttccgttc cttgcgtgat
      361 gaaaatgcga ccaggttgag ccatggattc cattcgggcg gcgacattga cggcatcacc
      421 gaacacgtcg ttgttcttca tgaaaaccgt tccggtgttg atgccaatgc gaatcaggat
      481 cttctcatca ctcgaccggt tgaactcgat cagagcctgc tgcatttcct gggcggcata
      541 acaggcttcc agcgggtcat cgtatttcac catcaacgca tcgccgatct ttttgatcaa
      601 ctgcccctgg tgtttcgcga tgatcggcat cagcgtgcga tcgtgcacgg ccagcatcgt
      661 catggtatcg agaagctctt tttcagcagc cttggccgag tacccgacaa tatccgtgaa
      721 catgatcgtt ttctcgaacg tgaatttttt gagcaactgt tcgtcaagct tcttgccggc
      781 cgtaccatct gaaaaccgtt tggccagaag caactcgatg ttcttgtccg aaaacttttc
      841 cagttccatt ttctgcagac gcattttgcg gaacatgtat tcggaactcg gaatcgttct
      901 cgctttgtac agcaggtcga gtcgactgat ctccttatcg aagtctcggt cgatgaaaga
      961 ttcatccgcc tgaatcgcgt tgatactgcg ctctttcaac aacttgtcga gctcgagtcc
     1021 gtctgccagc aataatggcg gaatcttcac ctggtcatga tgggtttcga tatcgagcgc
     1081 catggtctgc tgcgcctgaa tgctggtaat acgcgacatc tgcccaaacg aaaccggacg
     1141 aatcaccgcc cggctcgact gcacatgatc acgcaaaaag agaatcaacc ggcgattggt
     1201 cagcaccacc aacaggtaga ccggccgttc cagctgtttg atcatggttt cgatttcgcc
     1261 atactgttcc agcaccggag tggcatggtt gagattcaca atcgccaccg tcgtatgact
     1321 gtcgagagat cgcttgccat cgcgaaatgg tttgaaggca tcgatataaa aaccgatata
     1381 ggctcgatcc acggtttcat tctgcgtcag cgaattcaga atcatcccct gcgtatcttc
     1441 acgtaacgcg ttgagcaacc caatggcttt gggaaacggt ccatcccatt caccatcacg
     1501 ttcggcccgt ccctgacggt ttgagagctc tttcgacatc tgcggatacc cggtctcgct
     1561 cttgtattcg accatctgtg gccgatccgt cgtgaaaccg ggaaagcgct cttcgacctt
     1621 cttggttaac tcgtccagcg agagcccttt cggatcaaca aaatagggtt ccttcgcata
     1681 cggggaagac gccattcgtg ccgtcatgcg tatttctgag tcctgatcga gcagagcgtg
     1741 aagcaccaga tagtaatcct cctggcgcag ggtggtctgc tgtttgacat gcatgagcgc
     1801 cgccagcttc t
//
LOCUS       DAORHD010001108         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84606_length_1811_cov_1.818337, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001108 DAORHD010000000
VERSION     DAORHD010001108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84606_length_1811_cov_1.818337"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="PKO06_19105"
     CDS             complement(<1..1043)
                     /locus_tag="PKO06_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71821.1"
                     /translation="MSESLDSDIGSDSLEEWPRELAWENVRAVPSYHWEPWFAQQVRL
                     QVDAFQPDLIAVEIPDLFENAVHEAVACLPEMTAVWTGSRHLIPISPEDSIVEALRLG
                     KERGIPTACIDLETVVGRDSEGVAGVRGLADPVLLEAMSLQKFIDLNSEIIRADTPTP
                     ACRKREQNMAYHLRLLSKKYQRILFICGMIHWERIRALLSSEKPLERLRHEGGHALGL
                     VHVPPPMYFKITAGLGIPARALAWEHSRGGRPFTHLGWLSQVFLGAARELDRSMTPLD
                     LKAFLQYSRHRGIVSTNFRRAGMIVDAAQSIMDEEFGDEVRRQLLTYPWHPDPGKFPF
                     TVGYGMTECAPLI"
     gene            complement(1055..>1811)
                     /locus_tag="PKO06_19110"
     CDS             complement(1055..>1811)
                     /locus_tag="PKO06_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV71822.1"
                     /translation="MRVALEQKLEAAWLRVDETDVPMNPVVVGPPGCGKTSLIAAMAQ
                     KRNQPLYIYQCTMDTRPEDLIITPVLEGDQKIRYQASSLVSAMIAGGICILDEGNRMR
                     DKAWASLASLLDTRRYVESIIAGIKIPAHRDFRGASTMNEDTSTFQLPDYIRSRLRPR
                     IEVPEPDEATLEQVIRSNHPYVTEPVVSAVTKYLAKRRQENAPTVSTRQAVQLAAYTF
                     KRQSLGETGSLPELIHDGFQIIVDTETRSNRKA"
BASE COUNT          367 a          552 c          508 g          384 t
ORIGIN      
        1 catatcagcg gagcgcactc ggtcatgccg taacctaccg tgaaggggaa ctttcccgga
       61 tcgggatgcc agggataggt caacagttgc ctccgaacct cgtcaccaaa ctcttcatcc
      121 atgatcgatt gcgccgcatc aacgatcatc ccggctctcc gaaagtttgt gctgacaatc
      181 ccacgatgtc ggctgtattg cagaaaggct ttcagatcga gcggggtcat cgatcggtca
      241 agctctcgag cggccccgag aaagacctgc gacagccatc cgagatgggt gaacggtctt
      301 cccccgcggc tgtgttccca ggccagtgcc cgcgccggaa tgccgagccc ggcggttatc
      361 ttgaaataca tcggcggcgg cacatgcaca agccccaggg catgcccgcc ctcatgtctg
      421 agcctttcca gaggtttttc agaactcagc aaggctctga tcctttccca atggatcatg
      481 ccgcagatga agagaattcg ttgatacttt ttggataaca ggcgcagatg ataggccatg
      541 ttctgctcgc gcttgcgaca cgcgggcgtc ggggtatcag cccggatgat ctcactgttc
      601 aggtcgatga acttctgcaa actcattgct tcaagcagaa ccgggtcagc gagaccccgc
      661 acaccggcaa ccccctcgga atcacgaccg accacggttt cgaggtcgat gcaggcggtt
      721 ggaatgcccc gctctttgcc caatctcaat gcttcaacaa tcgaatcttc cggagaaatc
      781 ggaatgaggt ggcgactgcc ggtccagacc gcggtcatct cgggcaggca ggcaacggct
      841 tcatgaacgg cgttctcaaa cagatcgggt atctcgaccg cgatcagatc cggttggaag
      901 gcatcgacct gcagacgcac ctgctgagca aaccatggct cccagtggta gctcggcacg
      961 gcccgcacgt tttcccaggc aagttcccga ggccactctt ccagggaatc agagccgata
     1021 tcactgtcca atgactcgga catcttgacg ttcctcaggc ttttcgattc gatcgggttt
     1081 ccgtatcgac gatgatctgg aatccatcat gaatcaattc cggaagactg ccggtttcgc
     1141 ccagagactg ccttttgaag gtatacgccg ccagttgtac ggcctggcgg gtcgaaacgg
     1201 tcggcgcatt ttcctgacgc cgtttcgcga gatactttgt cacggccgag accaccggct
     1261 cggtcacata cggatgatta ctgcgaatca cctgttcaag cgtggcttcg tcgggctccg
     1321 gaacctcgat gcgcggccgc agacgcgagc gaatgtagtc gggcagttgg aacgtgctgg
     1381 tgtcttcgtt catggtcgaa gcgccgcgaa agtcccggtg cgccggaatc ttgatgcccg
     1441 cgatgatgct ctcgacatac cgacgcgtat cgagcaatga agccagcgac gcccacgcct
     1501 tgtcgcgcat gcggtttccc tcgtcgagaa tacagatgcc gccggcgatc atggccgata
     1561 ccagcgaact tgcctggtag cggatcttct gatcgccttc cagcaccggc gtgatgatca
     1621 ggtcttccgg gcgcgtatcc atcgtgcact ggtagatata cagcggctgg ttgcgcttct
     1681 gggccatggc ggcgatcagc gacgtcttgc cgcaccctgg cgggccgaca accaccgggt
     1741 tcatcggcac atccgtctca tcgactctca gccatgcggc ctcgagcttc tgttcgagcg
     1801 ctactcgcat c
//
LOCUS       DAORHD010001109         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84627_length_1810_cov_3.815385, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001109 DAORHD010000000
VERSION     DAORHD010001109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84627_length_1810_cov_3.815385"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..878
                     /locus_tag="PKO06_19115"
     CDS             84..878
                     /locus_tag="PKO06_19115"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV71823.1"
                     /translation="MFFPLHDEHNSRIAIVPVVSYGLVILISLVFACQVMLESGGGVG
                     SARGFVLRHGLVPEPFLTGNPEYSCTVAVKIVEDGEVEHDLRTIDVRNSKLGLILMPF
                     SYIFLHGSLLHLISNLWFFWIFADNVEERMRSIPFAIFFLLTGAAGGLLHVLLHTDSD
                     TPLIGASGAVAGVMGAYIVLFPGNRVTSYFCPVWFFIRRIDVPAWMVLGFFLLIQSLA
                     LMNADTLMSRVAFDAHLGGFVAGMLSALMFRASPHASPDAAGKTSK"
     gene            complement(820..>1810)
                     /locus_tag="PKO06_19120"
     CDS             complement(820..>1810)
                     /locus_tag="PKO06_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71824.1"
                     /translation="LSSQATAELTTQAKRIETLEEHLPSAQTTVLPATDAPVSEIFDL
                     TMRLYQKHVIALSLLSVAHCAVFMLLLFLGMFGLHSTGLRPSDLRDLKEVFDSPHAGA
                     AVGFLLWAALSAHCTFASLKAHLIIFLREYVHAGKFAYTALIGEAISWIPSTAWIQGR
                     KYLYLIGWLFLAGLAGRTCEVVLAVGPSASVLRRAFSDLAAMLAAFTVFAYGFLSYTM
                     AEPLQILQSESESVDALTRSRRMTGPWIFKLFLLCAFSMLLVQFITGATQELLLLGTF
                     FSASERFETLIRRATQLVAFLSQICLSPLLIANLVIYSFSLLHQAKHAEKHGT"
BASE COUNT          400 a          481 c          533 g          396 t
ORIGIN      
        1 ggcgagagaa cgaaatgccc cttcgattgt cgggagaggc ttgccacctg atcgatggtc
       61 gcctatactg agcgcggtta cccatgtttt ttccgcttca cgacgaacat aactcccgca
      121 tcgccatcgt tccggttgtc tcctatgggc tggtgatcct gatttcgctc gtttttgcct
      181 gtcaggtcat gctggaatca ggtggaggcg ttggctcggc acgtggtttt gtgctgcggc
      241 acggactggt gcccgaaccc tttctgaccg gaaatcctga gtattcctgc actgtggcgg
      301 tgaaaatcgt cgaagacggc gaagtggaac acgatcttcg cacgatcgac gtgcgcaact
      361 cgaagctggg gctgattctg atgccgtttt cctacatctt tctgcacggc agtctgctgc
      421 atctgatcag caatctgtgg ttcttctgga tcttcgccga caatgtcgaa gaacgcatgc
      481 gctcgattcc gttcgcgatc ttcttcctgc ttacgggcgc tgccggggga ttactgcatg
      541 tcttactgca taccgactcg gacacgccgc tgatcggggc gagcggcgcg gtggccggag
      601 tcatgggcgc gtatatcgtc ttgttccctg gaaaccgcgt gacgagctac ttctgcccgg
      661 tctggttctt tattcggcgt atcgatgttc cggcctggat ggtgctcggg ttctttctgt
      721 tgattcaatc tctggccctg atgaatgcgg acacattgat gtcaagagtg gcattcgatg
      781 cgcatctggg cggatttgtc gccgggatgc tctcggcact tatgttccgt gcttctccgc
      841 atgcttcgcc tgatgcagca gggaaaacga gtaaataacg agattggcga tcagcagagg
      901 ggagaggcag atctgagaga gaaacgcgac cagctgcgtc gcacgacgaa tcagcgtttc
      961 aaaacgttca gaggcggaaa agaacgtgcc gagcaacagc agttcctgag tcgccccggt
     1021 gataaactgc accagcagca tcgaaaacgc gcacagcaga aacagtttga agatccatgg
     1081 cccggtcatt cgtctcgagc gggtgagagc atcaaccgac tcggactctg attgcagaat
     1141 ctgtaaaggt tccgccatcg tgtagcttaa gaagccgtag gcgaatacgg tgaaagccgc
     1201 cagcatggcc gccagatcgc tgaaagcccg ccgcaaaaca cttgccgatg gcccgacggc
     1261 caaaacaact tcacaggttc gacccgcgag tccggcaaga aacaaccagc cgatcagata
     1321 cagatacttt cgtccttgaa tccaggcggt tgatggaatc cacgagatcg cttcgccgat
     1381 cagagcagta taggcaaact ttccggcgtg gacatattcg cgcaaaaaga tgatgagatg
     1441 tgccttgagg gaggcaaaag tgcaatgagc gctgagagct gcccacagca gaaaaccgac
     1501 agccgcgccg gcgtgcggac tgtcgaatac ctctttcaga tcgcgaagat cactggggcg
     1561 caaccctgtg ctgtggagcc cgaacatgcc gagaaacagc aacagcatga agacggcaca
     1621 atgcgcgacc gataacaggg aaagagcgat gacatgcttc tgatacagtc gcatggtgag
     1681 atcgaagatc tctgaaaccg gagcgtcggt ggccggcagc accgtggtct gggccgaggg
     1741 cagatgctct tcgagtgtct cgatgcgttt cgcctgagtg gtcagttccg cggtggcttg
     1801 cgaactgagt
//
LOCUS       DAORHD010001110         1810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84629_length_1810_cov_3.753846, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001110 DAORHD010000000
VERSION     DAORHD010001110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84629_length_1810_cov_3.753846"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PKO06_19125"
     CDS             <1..197
                     /locus_tag="PKO06_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71825.1"
                     /translation="GILLAKRLRELKPELVLVAMTGFSEELTPETIAESGFSRLVYKP
                     IIGAKLADAIRSALDEHQRK"
     gene            203..610
                     /locus_tag="PKO06_19130"
     CDS             203..610
                     /locus_tag="PKO06_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71826.1"
                     /translation="MPKILIVDDDPQVRSLLTERFQKAGFEAFSAENGRQGMKMLSTS
                     LPDVVITDLIMPEQEGIETIMQLRRDYPQIKIIAISGGIRGGTVNFLSAARELGASKV
                     YKKPFQFQELLAGVHELLGTTPAPRSDSEKPSE"
     gene            733..1314
                     /locus_tag="PKO06_19135"
     CDS             733..1314
                     /locus_tag="PKO06_19135"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNV71827.1"
                     /translation="MIKTEPIPVDELLEQIPAGAPLGIIGCGDCAAALHTGGTRQVDA
                     LREACAGRNPIAFATVATAPCDQRVLRRLIHLIQGFEQAQTIVLLACPAGAQSLATLL
                     QHEKISARLVLGLKTTGLGWIEKNGKTHPGCTFCASCTYDPLQGNCPTARCPLRRQDG
                     PCQNRLADDGCPVTPDLRCVWLDLPNSEGTSHE"
     gene            1307..>1810
                     /locus_tag="PKO06_19140"
     CDS             1307..>1810
                     /locus_tag="PKO06_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71828.1"
                     /translation="MNERYPPRWALCDCPSQMSEWESVTAFAVRHRVSLLLHAGRGLN
                     PFVLLQRSIPEIPELGLWVHLRDKTMPALESDLRTAVLLGVKTIFVGDPLPLAAVTND
                     SSPAGTMHVPAFLARAVQITGSSVRYGTVCRGSHPADVRLLQQLIAAGCAIVALPPQA
                     WEPSARQG"
BASE COUNT          348 a          540 c          543 g          379 t
ORIGIN      
        1 ccggtatact gctcgccaag cgactgcgtg aactcaagcc agagctggta ctggtggcga
       61 tgacgggttt cagtgaagaa ctgactccgg aaaccattgc ggagtctggt ttctcgagac
      121 tggtctacaa gccgattatc ggagccaagc ttgccgacgc cattcgttcg gcgctcgatg
      181 aacatcagag gaagtaacac tcgtgcccaa aattttaatc gtcgatgatg atccccaagt
      241 tcgttccctg ttgaccgagc gttttcagaa agccgggttt gaagccttca gtgcggaaaa
      301 tggccgccag ggcatgaaga tgctcagcac gtctctgcct gatgtggtca tcacggacct
      361 catcatgccc gagcaggaag gcatcgagac aattatgcag ctgcgtcggg actacccgca
      421 gatcaagatt attgccatat ccggtggtat tcgaggtggt acggtcaatt ttctttcggc
      481 ggctcgcgag ctgggggcct cgaaggtgta taaaaaaccg tttcagtttc aagagctgtt
      541 agccggagtt catgaacttc tcggcacgac acccgctccc cgctctgact ccgaaaaacc
      601 atccgagtaa ccgcgccggg cagacaatga cggtggtctg atctgtcgca ggtgctcgaa
      661 gtgcgccagg tatctgtggc attgcccgat tttttggggt agactgccga agcgcgccaa
      721 gcgtcattcg ttatgatcaa aaccgaaccc attccagtcg acgaactcct ggagcagatc
      781 ccggcagggg cccctctggg catcattggc tgtggtgact gtgcggccgc gctgcatacg
      841 ggcgggaccc gccaggttga tgcgttgcgc gaggcgtgcg ccggtcgcaa tcctattgcc
      901 ttcgccaccg tcgcgacggc accgtgtgat caacgggtat tgcgccgcct gattcacctg
      961 atacaggggt ttgagcaggc gcagacgatc gtcctcctcg cctgcccggc gggtgcgcag
     1021 agcctggcga ccctgctgca acacgaaaag atctccgccc gactggtgct gggtctgaaa
     1081 acgaccggcc tcggctggat cgaaaaaaac ggcaagaccc acccgggctg cacgttctgt
     1141 gcctcctgta cctacgaccc attgcagggc aactgcccca cggcgcgctg tccgttacga
     1201 cgacaggatg gcccgtgtca gaatcgcctc gcggacgacg gctgcccggt aacccccgac
     1261 ctccggtgcg tctggctgga tctgccgaac tctgaaggta caagtcatga atgagcgata
     1321 tccgccccgg tgggcgttgt gcgactgccc gtcacagatg tctgaatggg agtccgtaac
     1381 ggcgttcgcg gtccggcatc gcgtgagtct tctgctgcat gccggtcgcg ggttaaaccc
     1441 cttcgttctg ctgcagcgct cgattcctga gattcctgaa ctggggctct gggtacatct
     1501 gcgagacaag accatgccgg cactcgaatc agatctgcgc actgccgttc tgctgggcgt
     1561 caaaaccatt ttcgtcggcg atcctctgcc gctcgcagcc gttacgaacg actcaagccc
     1621 cgcgggcacc atgcatgtgc cggcctttct cgctcgcgcg gtgcagatca ccggctcgtc
     1681 ggtgagatac ggaacggtgt gtcgcggcag tcatccggcg gatgtgcgtc tgttgcagca
     1741 gctgattgcg gctggctgtg ccatcgtggc tctgccgccg caggcctggg aaccatcagc
     1801 caggcaggga
//
LOCUS       DAORHD010001111         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84738_length_1809_cov_3.073546, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001111 DAORHD010000000
VERSION     DAORHD010001111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84738_length_1809_cov_3.073546"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1809
                     /locus_tag="PKO06_19145"
     CDS             <1..>1809
                     /locus_tag="PKO06_19145"
                     /inference="COORDINATES: protein motif:HMM:NF015148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HNV71829.1"
                     /translation="GFLYKVLMSGFKVWSSEPGYKLEWYKGTHVSGELAPELLGVGYI
                     IGPRTAGIMMGGGVLSTLVLIPLIKMFGEHLKEPLFPGTTLISAMETHDIWKSYVLYI
                     GAGAVAAGGIISLFSSLPLIFSAFRAGLKDFSIGGSPTQAQTPRTDRDLPLTVVVGGT
                     LLLVALVWLSPMLNVTLPGAVLIVMFGFLFVTVSSRLVGEIGSSSNPISGMTVSTLLI
                     TCLIFLFMGWTGAEYRLTALSIAAVVCIAASNGGTCSQDLKTGFLVGATPKWQQISIL
                     IGALTSAVVIGGTLLALNSASTVYVRPQAGLKFRADVSKLTKKESVRGPEAVRDTRVY
                     SVLYLNEANDELPAGKYLINDDGSIAWIIDPGINGTAKFHECNRQFQKIEDPAAQAFA
                     LPAEKLAELEVVNAEVASSGGLPLDQTYRTLLLDKKTDGVEAGKYLVDGTGKLRFKVD
                     QGPEVTKYDSPKAKLMSLIIDGILTQKLPWILVIIGAFIAVLLELCQVSSLPFAVGVY
                     LPFSASVPIFIGGMVRWLVDATSKKLSDAESESSPGVLVSSGLIAGGSIAGIALALMA
                     LYENALAKLDCSKLLTEATSSLVSAVLFVLMALFLYA"
BASE COUNT          321 a          490 c          556 g          442 t
ORIGIN      
        1 cgggtttctg tacaaggtgc tcatgagcgg attcaaggtc tggagctcgg agccgggata
       61 taaactcgag tggtataaag ggacccacgt gtcgggcgag cttgccccgg aactgctcgg
      121 cgttggttac atcatcggcc cccgaaccgc cggcatcatg atgggtggcg gtgtgttgtc
      181 gaccctggtg ttgattccgc tgatcaagat gttcggtgaa catctgaaag agccgctgtt
      241 ccccggaacc acgctgattt ccgccatgga gacgcacgac atctggaagt cttacgtact
      301 ctacatcggg gcgggagcgg ttgccgccgg tggcattatc agtctgtttt cctcgttacc
      361 gttgattttc agcgcctttc gcgccggtct gaaagacttc tcgatcggcg ggagcccgac
      421 gcaggcgcag actcctcgca ctgatcgcga tctccctctg acggtcgttg tcggtggcac
      481 gctgttgctg gtcgcgctcg tgtggctgtc gcccatgctg aatgtgacct tgcccggtgc
      541 ggtgctgatc gtcatgttcg gttttctctt cgtcaccgtc agttcgcggc ttgtcggcga
      601 gattggttcc tcctcgaacc cgatctcggg aatgaccgtt tcgacgctgc tgatcacctg
      661 tctgatcttt ctgttcatgg gttggactgg cgctgagtat cgtctgacgg cgctgtcgat
      721 cgcggcggtg gtgtgcatcg cggcctcgaa tggtggcacc tgctctcagg atctcaagac
      781 cgggtttctc gttggggcga ccccaaaatg gcagcagatc agcattctga tcggtgcgct
      841 gacctcggct gtggtgatcg gtggaaccct gctcgccctt aactctgctt ctacggtcta
      901 tgtgcgaccg caggctggtc tcaaattccg cgccgatgtg agcaagttga ccaaaaagga
      961 atcggtacga ggccccgaag cggtgcggga tacgcgagtc tattccgtgc tgtatctgaa
     1021 cgaggcgaat gacgagttgc cggcggggaa atacctgatc aacgacgatg ggtcgattgc
     1081 ctggatcatc gatccgggta tcaacggcac ggcgaagttc cacgaatgca accgccagtt
     1141 tcagaaaatc gaggaccctg cggcgcaagc gtttgcgctg cctgccgaga aactcgcaga
     1201 actcgaggtt gtaaacgcgg aagtcgcctc ttccggcgga ctgcccctcg atcagaccta
     1261 tcgcacgctg ttgctcgaca agaagaccga cggggtcgag gcgggaaagt atctggtcga
     1321 tggcacagga aaactgcgct tcaaggttga tcagggtccg gaagtcacga agtatgattc
     1381 tccgaaagcg aagctgatgt cgctgatcat cgatggcatt ctgacgcaga agctgccctg
     1441 gatcctcgtc atcatcggcg ctttcattgc cgtgttgctt gagctgtgtc aggtctcgtc
     1501 gctgccattc gcggtcggtg tgtatctgcc gttctcagct tcagttccca tcttcattgg
     1561 cggcatggtg cgctggctgg ttgatgccac ctcgaaaaaa ctgtccgatg ccgaaagtga
     1621 atcaagcccc ggagtgcttg tgagttcggg cctcatcgcg ggtggatcga ttgccggtat
     1681 cgcgctggcc ctgatggcgt tgtatgagaa tgcgctggcg aagctcgatt gcagcaagtt
     1741 gctcactgag gctacttcaa gtctggtgtc ggctgtcctg tttgtgctga tggccctgtt
     1801 tctgtatgc
//
LOCUS       DAORHD010001112         1808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_84797_length_1808_cov_4.482601, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001112 DAORHD010000000
VERSION     DAORHD010001112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84797_length_1808_cov_4.482601"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="PKO06_19150"
     CDS             complement(<1..214)
                     /locus_tag="PKO06_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV71830.1"
                     /translation="MISELLQANFIDLDLECGSKQDAIDRLSNLLTSSGKVTDRTHFV
                     KAVMERERLGSTAIGAGIAIPHARANT"
     gene            complement(357..1766)
                     /locus_tag="PKO06_19155"
     CDS             complement(357..1766)
                     /locus_tag="PKO06_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV71831.1"
                     /translation="MSIEILIVDDEDMIRFTLRESLERQGYRVLDFANGEDFLAHFQQ
                     KGGDIVMLDIRLPGMSGLDVLKELKHLDASTPVIMMTAYGDVETAVTAMKLGATDYIS
                     KPYSLAEVHLILRNLADHSILKRQLSHYQEKIDGNYTQLLGDNVKMKTIREHISMAAQ
                     SDKTTVLIRGESGTGKELVARQIHNQSARRNKPFIDVNAAAVTGTLLESELFGHEKGA
                     FTDAQKQKKGVFELADGGSLFLDEIGDLSPSLQAKLLRVLQEKRFRRVGGSVDISVDV
                     RIIAATNADLEKQIEAGTWRRDLFYRLNVFTIQLPPLRDRRDDVLLLARFFLDQYNRE
                     FSKKITGFAPETTEILMSYPFPGNVRELKNLIERATLLETSALLKPGSLPEEMRSIRP
                     RVSVPVAETAAGGTLPAQPWLQAGFSFKDYIDGIEKQVIEQALTATQGKKGDAARLLG
                     MTRFAFRHQLKKHNIADDE"
BASE COUNT          384 a          483 c          519 g          422 t
ORIGIN      
        1 cggtattcgc gcgggcgtgc ggaatggcaa tgccagcgcc aatggcggtg cttcccaggc
       61 gttcacgctc catgaccgcc ttcacgaaat gggtacggtc cgtgacctta cctgaggaag
      121 tgagcagatt ggacaagcgg tcgatggcat cctgcttcga gccacactcg agatccaggt
      181 cgatgaagtt cgcttgaaga agttctgaaa tcataatccg ctccttttcg atgtctcaca
      241 tggaattagc aggtttcgtg ccaagcggaa aagcgagtgt acaagccaaa acctcaaaaa
      301 agagagtgaa aaaaatgcac ctgcaagggt gggtgtgaaa aaagcacacc cccgcactac
      361 tcatcgtcag caatgttgtg tttttttaac tgatgtctga aggcgaaacg cgtcattccc
      421 aaaagccgtg cggcgtcgcc ctttttccct tgggttgcgg tgagagcttg ctcgataacc
      481 tgcttttcga taccgtcgat gtagtccttg aaagaaaatc cggcttgcag ccagggctgt
      541 gcaggcaggg ttccgccggc ggcggtctcg gcaaccggaa cactgacgcg cggtctgatg
      601 ctgcgcatct cttcgggcag gctgccgggc ttcagcaggg cagaggtttc aagcagtgtc
      661 gcccgctcaa tgagattctt cagttcccgc acattgccag gaaatggata gctcatgaga
      721 atctctgtgg tctcaggcgc aaagccggtg atcttttttg agaactctcg attgtactgg
      781 tcgaggaaga agcgggccag cagcagcacg tcgtcacgac ggtcacgtaa tggtggcagt
      841 tgaatggtga agacattcag acgatagaac aggtcgcgtc gccacgtgcc ggcttcgatc
      901 tgtttctcga gatctgcgtt ggtggctgcg atgatgcgca catcgacgga gatgtcaacg
      961 gaaccaccga cccggcgaaa ccgcttttcc tgcaggacgc gtaacagttt agcctgcagg
     1021 ctggggctga gatcaccgat ctcatcgagg aagagagagc caccgtcagc gagttcgaaa
     1081 acgcccttct tctgcttctg cgcatctgtg aaggcgccct tttcgtgacc gaacaactcg
     1141 gactcaagca gcgtgccggt tactgccgcc gcgttgacat cgatgaacgg cttgttgcgt
     1201 cgtgcgctct ggttgtgaat ctggcgggca acgagttctt tgcccgtacc tgattcgcca
     1261 cgaatcagta cggttgtctt gtcgctctgt gctgccatcg agatgtgctc tcgaatggtc
     1321 ttcatcttca cgttgtcacc gagcagctgg gtgtagttcc cgtcgatctt ctcctggtag
     1381 tgtgacaact ggcgcttcag gatcgagtgg tccgcgaggt tgcgcagaat cagatgcacc
     1441 tcggcgagac tgtagggttt tgagatatag tcggtcgcgc cgagtttcat tgccgtcacg
     1501 gcggtttcaa cgtccccgta tgccgtcatc atgatgaccg gcgtcgaggc gtcgaggtgt
     1561 ttcaactctt tcagcacgtc gagcccgctc atcccgggaa ggcgaatgtc gagcatgacg
     1621 atgtcgccgc ccttctgctg gaaatgcgcg aggaagtcct cgccattcgc gaaatccaga
     1681 acccggtatc cttgcctctc gagcgattca cgtagagtga atcgaatcat gtcctcgtca
     1741 tcgacgatca gtatttctat tgacataaat taacgtgcag agcgagccct cctcatcagg
     1801 ggtagcag
//
LOCUS       DAORHD010001113         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85042_length_1805_cov_3.373143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001113 DAORHD010000000
VERSION     DAORHD010001113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85042_length_1805_cov_3.373143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="PKO06_19160"
     CDS             <1..1226
                     /locus_tag="PKO06_19160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71832.1"
                     /translation="VAGAFKPHGIKAFVIAGGPHPTFFPEFVETEGIDAINIGEGELS
                     MLELADAVSRKQPVDEIRNLVIKTSEGIRRNPLRPQVDINAMPLPDRSIYLAYDYFKG
                     KDSQDFVVSRGCPYSCSFCFFHKWNEMYKDTSGFTPFRLKTIDRCMQEIQDMRSQARL
                     PFVSYVDSTFNLDRAWTLEFLKRYRETVEIPFTMNLRPNLVDEEIISAVARTGKCRII
                     RMGIEVGNESYRMNVLRKHVTNDQIRTAGELLRKYSIKLMTYNMYCLPGQTLEQALET
                     VEINQFLKPAAISSMIFHPYPGLDLTKYALEKGFLKLEDISKLEIREYKWFRSVMSQP
                     ELREVQNLCLLSRVGVRYPKTVPLLKHLVRYPSNKAYDWVALAGLIEMAREYYDISTW
                     RVVQQHLIGDKSKMN"
     gene            1302..1517
                     /locus_tag="PKO06_19165"
     CDS             1302..1517
                     /locus_tag="PKO06_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71833.1"
                     /translation="MPDLKTHLAITYEPTGPYGAKSVSEIGINGPLPALANAIYDAVG
                     VRLVKPPFTAEKILAGIKGLKKSGQWK"
BASE COUNT          435 a          473 c          490 g          407 t
ORIGIN      
        1 aggttgccgg ggcgttcaaa cctcatggaa tcaaagcgtt cgtcatcgcg ggggggccgc
       61 accctacatt ctttccggag ttcgtcgaaa ccgagggaat tgacgcgatc aacatcggcg
      121 agggggagtt gtccatgctg gagttggcgg acgccgtttc tcggaaacag ccggttgatg
      181 aaatccggaa cctggtcatc aagacctctg aaggaatcag acgaaacccg ctgcgaccgc
      241 aggtagacat caatgccatg ccgcttccgg accgatccat ctatcttgcc tacgattatt
      301 tcaagggcaa ggactcgcag gatttcgtcg tgtctcgcgg ttgtccctat tcctgttcct
      361 tctgcttctt tcataaatgg aacgaaatgt acaaggacac aagcggattc acgccgtttc
      421 gtctgaagac gattgaccgg tgtatgcagg agattcagga catgcgttcg caggctcgat
      481 taccattcgt cagttacgtg gacagcacgt tcaacctaga ccgtgcctgg acacttgaat
      541 tcctgaaacg ctatcgggaa accgtcgaga ttccgtttac gatgaacctg cgccccaacc
      601 ttgtggatga agagatcatt tctgccgtcg cgcgcaccgg gaagtgtcgg attattcgaa
      661 tgggtattga agtcgggaac gaatcgtatc gcatgaacgt gttacggaaa catgtgacca
      721 acgatcagat tcgcacggcc ggggagttgt tgcgcaagta cagcatcaaa ttgatgacct
      781 acaatatgta ctgtttgcca gggcagacac tcgagcaggc actggaaacg gttgaaatca
      841 atcagttcct gaaaccggcc gcgatttcct cgatgatttt tcacccgtat ccaggactcg
      901 atctcaccaa gtatgctctg gaaaagggtt ttctcaaact cgaagatatc tcgaagctcg
      961 aaatccgaga atacaagtgg tttcgaagcg tcatgtcaca gccggagctc cgagaagtgc
     1021 agaatctttg tctgctctcg cgggttggtg tgcgctatcc gaaaacagtg ccgctgttga
     1081 aacatctggt ccggtatccc tcgaacaaag cctatgactg ggttgccttg gctggcttga
     1141 tcgaaatggc gcgagaatac tacgacatca gcacctggcg ggtggtacaa caacacctga
     1201 tcggcgacaa gtccaaaatg aactgagcga ctcctgtgcg ttgcattcac tgatcagaaa
     1261 tcacggttcc tacgtatcga gccgccggta acgtgaacga tatgcccgac ctgaagacgc
     1321 atctggccat cacctacgag ccgaccggcc cgtacggagc caagagcgtc agcgaaatcg
     1381 gcatcaacgg cccattgccc gcgctcgcca acgcgatcta cgatgcggtc ggcgtgcggc
     1441 tggtgaagcc cccgttcacc gctgagaaga ttctcgccgg catcaagggc ctcaagaaat
     1501 ccggtcagtg gaagtaatct agactcgctc gcaatgcggg cggtgggccg acaggctcac
     1561 cgcccgcttc ttttgtcgcg ggtttcaaca cggcatctca tgttttgtgt gaaaagacgc
     1621 ggagactggg gggcggtgag gaagcactcg atgagttttt gccgacgatt aaaaaccgaa
     1681 aatcattctg cagatccctg actaagttcc aagctgccgc tcggcagaaa tgaccggcca
     1741 gaatcctctt tcaggaaaag ggtcagattt ctcttttcaa tgtgtcggcg atgaatttgt
     1801 tcagg
//
LOCUS       DAORHD010001114         1805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85070_length_1805_cov_2.749143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001114 DAORHD010000000
VERSION     DAORHD010001114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85070_length_1805_cov_2.749143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKO06_19170"
     CDS             complement(<1..339)
                     /locus_tag="PKO06_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV71834.1"
                     /translation="MPTFTYKARNWDGKIVTAEMEGESKETCIAKLREKGYFVTSINE
                     KKGGGGISLNFFERGISSTEVCIFARQFATMIGSGVPLVRCLTILQQQSENPTFKRVI
                     SQIRADVEGGS"
     gene            611..>1805
                     /locus_tag="PKO06_19175"
     CDS             611..>1805
                     /locus_tag="PKO06_19175"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNV71835.1"
                     /translation="MKHLVFAEKPSVARDIARVLGARQRANGYFEGEHWIVTWGVGHL
                     ISLAEPGEQNPSWSKWNLQALPMLPDTWKLKVLEQTAAQFAIVKALMGRPDVADIVNA
                     ADAGREGELIFRYVYEAAAAAKPTQRLWISSMTDEAIKQGFKSLKPGKDFDNLGQAAR
                     CRSIADWLIGMNGTRGYTKKFNTMLTVGRVQTPTLAMVVKRQLEIEAFKPEPYWEITV
                     RLDDFQAQWFDPKHPEKENAARLLDRKKAEAIVALLKGLPAVVTRTRSETKKIPPPLL
                     YDLTTLQREANRFFGLTAAQTLQIVQTLYEQRKVVTYPRTDSQHLSDDLHATIPDRLK
                     ALPSEYQLFTRELSQQPIPKNKRVFDNAKVSDHHAIIPTEKPAQDIRSWKPDEQKIYD
                     LIARRF"
BASE COUNT          425 a          515 c          494 g          371 t
ORIGIN      
        1 tgaaccgcct tcgacgtcgg cacgaatctg gctgatgaca cgtttgaagg tcgggttctc
       61 tgactgttgc tgcaaaatcg tcagacagcg aaccagcggc acgcctgatc cgatcatggt
      121 ggcgaactgg cgggcaaaga tacacacctc agtcgacgag ataccacgtt cgaagaagtt
      181 caggctgatt ccgccgccgc ccttcttctc gttgatggaa gtgacaaagt accctttctc
      241 gcgcagtttc gcgatacagg tttctttgct ttcgccttcc atttcggccg tgacgatctt
      301 gccgtcccag ttgcgggctt tgtaagtgaa agtaggcata agcagccctc ctttatttca
      361 cacgcgaagc gtgttgcaca gtctggtcat ccatcaccag tcgttgcaga aaacatgcca
      421 gagttgaagc aggaattcaa gcccctgaag cggtttttcc gacaacagca tgtgcggatt
      481 tccgcacaca gaggtgtgcg aaaccctgag tacagtatcg gcatgtcagg ctttttcgcc
      541 gagagaaata ccgatctgcg cccttcccgg tgtatggaca attttttccg aagcgtctac
      601 aatcggcttc atgaagcatc tcgtcttcgc ggaaaagcct tcggtcgcgc gagatattgc
      661 gcgagttctg ggggcacgac aacgagccaa cggatatttt gaaggggaac actggatcgt
      721 cacctggggt gtaggtcacc tgatctctct ggctgaacct ggagaacaga accccagctg
      781 gtcaaagtgg aacctgcagg cattgccgat gcttcccgac acctggaaac tgaaagtgct
      841 cgagcagacc gcggctcagt ttgccatcgt caaagcactg atggggcgac cggatgtcgc
      901 tgatatcgtg aatgccgcag acgcggggcg agagggcgaa ttgatctttc gttacgtgta
      961 cgaagcggcg gcggcggcga aacctaccca gcgtctgtgg atctcgagta tgaccgacga
     1021 agccatcaag caaggcttca agagcctgaa accggggaaa gatttcgata acctcggcca
     1081 ggccgcccga tgccgcagta tcgccgattg gttgatcggc atgaacggaa cccgcggcta
     1141 caccaagaaa ttcaatacca tgctgaccgt cggccgcgtg cagaccccca ccctggcgat
     1201 ggtcgtcaaa cgccagctcg agattgaagc cttcaaaccg gaaccctact gggagatcac
     1261 ggttcggctc gacgactttc aagcacagtg gttcgatccg aagcaccccg aaaaggaaaa
     1321 tgcggctcgg ctgctcgacc gtaaaaaggc ggaagcgatt gtcgccctgt tgaagggctt
     1381 gccggcggtc gtaaccagga ctcgttccga aaccaaaaaa atccccccgc cgctgctgta
     1441 tgatctcacg accctgcagc gcgaggccaa tcgcttcttt ggtctgacgg cagcgcagac
     1501 tctgcagatc gtgcagaccc tgtatgaaca gcgcaaggtg gtgacgtatc cgcgcacgga
     1561 cagtcagcat ctgtctgacg acctgcatgc cacgatcccc gatcgactca aggcccttcc
     1621 gtcagagtac cagttgttta cccgcgagtt gagccagcag ccaataccca agaacaagag
     1681 ggttttcgac aacgcgaaag tcagtgacca tcatgccatc atccccaccg agaaaccggc
     1741 gcaggacatt cgctcgtgga agcctgatga gcagaagatc tatgatctga tcgcccgacg
     1801 ctttc
//
LOCUS       DAORHD010001115         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85132_length_1804_cov_3.267010, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001115 DAORHD010000000
VERSION     DAORHD010001115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85132_length_1804_cov_3.267010"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PKO06_19180"
     CDS             <1..688
                     /locus_tag="PKO06_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNV71836.1"
                     /translation="RKGDRAPRYVPGFKARDQEDFELEPGMERFRIEIGHRHHVLPGN
                     IVGAIAGESGLNSKFIGRIKIYQEYSTVDLPEGMPDHVLELLKRAWISGRQLNISRVT
                     PGDDRAEDQASARTPDADRPPRNAEGWTDAVPKDQKPRRRSAAEDTRETDRRPRSRRD
                     EHETKRAPRQSPGAREPLMGRSHSDRPPRFTREEKRPEGSRTRPSERPSSRRDQRAGT
                     GNKPPRRPTR"
     gene            complement(741..1586)
                     /locus_tag="PKO06_19185"
     CDS             complement(741..1586)
                     /locus_tag="PKO06_19185"
                     /inference="COORDINATES: protein motif:HMM:NF037817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5610 domain-containing protein"
                     /protein_id="HNV71837.1"
                     /translation="MVDKVSVLNPANAGTIGSSHRASAADLLQVANQSQRSDPFKGLN
                     LDQLTLSDDARQKIVWARTQFELSYQVIRSLNTSQGERITEESFSFSGSIEFLQRVSG
                     RDPVVLADPTAQPEAGASEDLNSTTSADALSALQEYFSPEKTAERILDVALSFFGVSE
                     TQAASGNTPASRQTFADFIGKAIEEGFTQARSLLGQLPEAVETGVSKTHSLVFDGLAD
                     FVQNGINPEKLAPGGVMEKIARYRREATENMDQIRKTTSAGGYNARGEPAEPSDKTPN
                     VSRLG"
BASE COUNT          390 a          510 c          556 g          348 t
ORIGIN      
        1 gagaaagggc gaccgggctc cgcggtacgt gccggggttc aaagcgcgcg atcaggagga
       61 ttttgagctg gaacccggca tggagcgctt tcgtattgaa attggacacc ggcatcacgt
      121 gctgccggga aacatcgtgg gggctattgc tggtgagtcc gggctgaaca gcaagttcat
      181 cggccgtatc aagatctatc aggaatacag taccgtagac ctgccggaag gcatgcccga
      241 tcacgtgctg gaactgctga aacgagcctg gatctcaggt cgtcagctca atatctcacg
      301 cgtgaccccg ggtgacgatc gagcagaaga tcaggcttcg gcgcgaactc cggatgccga
      361 ccgaccaccg cggaacgccg aaggctggac ggatgccgtt cccaaggacc agaaaccccg
      421 gcgacggtca gccgctgagg acacgaggga gacggacagg cggccgcgaa gccggcgaga
      481 cgagcacgaa accaaacgag caccgaggca atcgcctggt gcccgcgaac ctttgatggg
      541 gcgctctcat tccgatcgac cgcctcgttt cacccgcgag gagaagcgac ctgagggaag
      601 ccggacgcga ccgagtgaac gaccgtcgtc tcgccgtgat cagcgcgctg gcaccggaaa
      661 caagcccccg agaagaccga cgcgataggg cgtgcgattt cgggggcgta tcccgcagga
      721 tgcgaggaat gccgggccgg tcagcccaac ctggaaacgt tcggggtttt gtcagagggc
      781 tcggcgggct cgccccgcgc attgtaccca ccggcactgg ttgttttccg tatctgatcc
      841 atgttttcgg ttgcctcgcg ccggtaccgg gcaattttct ccatgacccc gccgggagcg
      901 agtttctcgg ggttgatccc gttttggaca aaatcagcga ggccgtcaaa caccagggaa
      961 tgggttttcg acacccccgt ttcgacggct tccgggagtt gtccgagcag agagcgggcc
     1021 tgagtgaatc cttcctcgat cgccttgccg atgaagtcgg cgaatgtctg acgtgacgcc
     1081 ggggtgttcc ctgacgcggc ctgagtctcg ctgacaccga aaaagctgag agcgacgtcg
     1141 agaattcgct cagcggtttt ttctggcgag aaatactctt gcagagctga taacgcatca
     1201 gcagaggtag tactattgag atcttcagag gcgcccgcct ctggctgtgc cgtggggtcg
     1261 gcaagaacga caggatctct gcccgaaact ctctgaagaa actcgatgct ccccgaaaag
     1321 gaaaacgact cttccgtgat tcgttctccc tgcgaggtat tcagggaacg aatgacctga
     1381 tagctcagtt cgaactgtgt gcgggcccag acgatcttct ggcgtgcgtc atcggaaagc
     1441 gtcagttgat caagattcag tcccttgaac ggatcactcc gttgactctg gttggccacc
     1501 tgaagaagat cagctgccga ggcgcgatgg gaagagccga ttgtgccggc attcgcagga
     1561 ttgagcacgg ataccttgtc taccatggtt tctcctcatt gatgaactac agaacatgca
     1621 gcacaatccg tgccagtcag acggtgtgag tgaccgactc gaaagcctgc cccacgggag
     1681 aagttgctac tcccagcgtt gccactcggg cttgccggcg tagacttctt cgaagtagga
     1741 cgtacgactc aacttcgatg cacctgccac gtcgagaatg atgatggcct gttcgtgaaa
     1801 ctga
//
LOCUS       DAORHD010001116         1803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85191_length_1803_cov_4.085240, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001116 DAORHD010000000
VERSION     DAORHD010001116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85191_length_1803_cov_4.085240"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /gene="hutI"
                     /locus_tag="PKO06_19190"
     CDS             complement(<1..870)
                     /gene="hutI"
                     /locus_tag="PKO06_19190"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNV71838.1"
                     /translation="METRKVAADILIHSLASAVLFPRPYRGKGTPVCWDIEERRDVSI
                     AGFRGKIAWIGPSAEICEHVTLLPGAELINAQGLTAIPGFVDAHTHLVFSGDRSNEFE
                     MRVQGRPYLDILAAGGGILKTVESVRQRSEDELFQEALERAWKVMAWGVTTLEIKSGY
                     GLDLDSECKTLRVVRRLKETLPISIVATFMGAHAVPKEAKSNPDAFVDRLCDEWIPVI
                     AREELARFNDVFTENKAFSVEQSRRILLAGQKHGLQPKIHADEINVLGGVDLAVEIGA
                     VSADHLLKTGLEGI"
     gene            1050..>1803
                     /locus_tag="PKO06_19195"
     CDS             1050..>1803
                     /locus_tag="PKO06_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71839.1"
                     /translation="MSIYPRRISFLNTILLITLSVILLQTEAIAGRTLVVHRPLVSFA
                     HFLYGTSGVFPPAAAFNRDLLAWKSVLDTSTAQALDRVALWFRSSIAKFETEHAFETA
                     YYQAAAAADDLDSLIRSLRTRGIVVHPPLREDFDRLSVLFQTHFAADQSLIRQELRRL
                     NALPETRHFRRILANMDRFYRPDLRQATPLGNIEIVAIPLRLTPVEMQDLRSKGKNSI
                     FGRNFGHLQIVELVLIPEVPDLARKMLSHLLGV"
BASE COUNT          369 a          538 c          474 g          422 t
ORIGIN      
        1 aatgccttcg agacccgtct tgagcagatg atcggcgctg acggcaccga tctcgacggc
       61 cagatcgacg ccgccgagta cattgatctc atcggcatgg atcttcggtt gcaggccgtg
      121 cttctgacct gccaggagaa tgcggcggga ctgctcgacg gaaaaggctt tgttctcggt
      181 gaagacatca ttgaaccggg ccaattcttc ccgggcgatg acgggaatcc actcgtcgca
      241 caaccgatcg acaaaagcgt ccgggttgct tttggcctct ttcggcacgg cgtgagcacc
      301 catgaatgtg gcaacgatcg aaatgggcag agtttccttc aggcgacgta cgacacggag
      361 tgtcttgcac tcactgtcga gatcaagacc atagccgctc ttgatctcaa gcgtcgtcac
      421 accccacgcc atgactttcc acgcccgctc cagggcttcc tgaaacagtt cgtcttcgga
      481 tcgctgacga accgactcaa cggtcttgag aattccgccg cccgcggcaa gaatgtcgag
      541 atagggccgc ccctgcacgc gcatctcgaa ttcgttgctg cgatcgcctg agaaaacaag
      601 atgcgtatgg gcatcgacaa aaccgggaat cgcggttaac ccctgcgcgt tgatcagttc
      661 cgctcctggc agcagagtca cgtgctcgca gatttcagct gagggtccga tccaggcgat
      721 cttgccgcga aagcccgcaa tcgacacgtc tctccgctct tcgatatccc agcatacggg
      781 ggttcccttg ccacgatatg ggcgcgggaa gaggaccgcc gaagcgagcg aatggatgag
      841 aatgtcagcg gcgacttttc tggtttccat gttcttgccc cctgtcaggt gttgttcgtc
      901 accttaacac aaacccaggg tcatgtgatt ctccggcttg ggtttccgcc ttggcatcgc
      961 tcaacgattc gccgtcgctg gatattcgct ggggaatggc ataaggtatc actggcctcc
     1021 aattcgtacc attctcggag atatcgagta tgtccattta cccgcgtcgc atctcgtttc
     1081 tgaacacgat tctgttgatc accctgtctg tcattctgct ccagactgag gcgatcgcag
     1141 gtcgaaccct cgtcgtgcac cgtcctctgg tcagcttcgc ccattttctc tacggcacgt
     1201 caggggtgtt tccaccagct gccgcattca accgtgatct tctggcctgg aagtccgttc
     1261 tcgacacttc gacggcgcaa gcgctcgatc gcgttgccct gtggtttcgc tcttcgattg
     1321 cgaagttcga aacggaacac gccttcgaaa ccgcctacta tcaggcagcc gcggcagcag
     1381 acgatctgga cagcctgatc aggtcactgc gtacacgtgg tattgtcgta cacccgcctt
     1441 tgcgagaaga tttcgatcgt ctctcagtgc tttttcaaac gcacttcgcc gcggatcagt
     1501 cactgatacg acaagaactg cggcgactga atgccttgcc agaaaccaga catttcaggc
     1561 gcattctcgc gaacatggat cgtttctacc gtccagattt acgtcaggcg actccgttgg
     1621 gcaacatcga aatcgtcgcc attcccttga gactgactcc cgttgaaatg caggatctgc
     1681 ggagcaaggg gaagaactcc atcttcggcc gcaattttgg acatctgcag atcgtcgaac
     1741 tggtactgat tccggaggtt cccgatcttg cccgaaaaat gctctctcat cttctcggag
     1801 tcg
//
LOCUS       DAORHD010001117         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85341_length_1801_cov_4.432990, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001117 DAORHD010000000
VERSION     DAORHD010001117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85341_length_1801_cov_4.432990"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKO06_19200"
     CDS             <1..464
                     /locus_tag="PKO06_19200"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV71840.1"
                     /translation="IGQRPQPSSLALTLAILQALMVKIGNEIYAIPLGSVQETMDLEP
                     QDISIVQHQEVTLLRGETLLPIIRLRQVLGVPTQDAEKEKNEVRSVVVCASGEKQAGF
                     MVDELLGQQEIVIKSMGNLISGLPGIMGATMRGDGSIALILDIPAFLQSKT"
     gene            508..>1801
                     /locus_tag="PKO06_19205"
     CDS             508..>1801
                     /locus_tag="PKO06_19205"
                     /inference="COORDINATES: protein motif:HMM:NF018851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNV71841.1"
                     /translation="MDLDLSQYMGPYIDGSRENLDTMDRQLLVLEQNPNSLEAVEEIF
                     RAAHTLKGMSATMGFEKVAHVTHEMENVLDKLRTKQMPVTPAVIDVVFETFDILRILV
                     NDSISATDSQVDLGGISQKLATIISSGGATSGGSAPAGQEEALSAAPEPGTTSAEPAA
                     GGSAGDEGGAPDLSEFGLSEFEMSGLADAQQSGNIPYLLVVTLVADCLLKGPRIFMVM
                     RSLDNLRCEVIKSVPDTKDLEAERFDRSFKMIITMKSTAQELKDSIESISEIERADVI
                     PAFGGGGAAVSVPPQAAAPLPVAVAAPVPAPVVATPPTPAAAPVTAPAKAAVTAPARA
                     AAAPMAPAAPPPVAAAGPDEPPAGAVPPGGKPPESDGKTQEAKEFLELTQLVSFRMAG
                     ETYALDIFLVEGIINLVPITRVPKAPPHIEGVINMRGEI"
BASE COUNT          394 a          550 c          516 g          341 t
ORIGIN      
        1 gcatcggtca gcgtccgcag ccgagctctc tcgctctcac gctcgcgatt ctgcaggctc
       61 tcatggtcaa gatcggcaat gagatttacg cgattcctct cggttccgta caagagacga
      121 tggatctcga gccgcaagac atcagcattg tacagcatca ggaagtcaca ctgctgcgcg
      181 gcgagaccct gcttccgatt atccgtctgc gccaggttct cggtgtgccg acgcaggatg
      241 ccgaaaagga aaagaatgag gtgcgcagcg tcgtcgtgtg cgcttcgggt gaaaagcagg
      301 caggattcat ggtcgatgag ttgctcggcc agcaggaaat cgttatcaag tcgatgggca
      361 atctgatcag cggattgccg ggaatcatgg gcgcgaccat gcgtggtgac ggcagtattg
      421 cgctcattct cgacatcccg gcattcctgc aatcaaagac ctgatgcagg ttatgatggg
      481 atacactcga agaaaggagg tggtggcatg gatctagatc tgtcccaata catgggcccg
      541 tacatcgacg gctcacgcga aaacctcgat accatggacc gccagctcct ggtgcttgaa
      601 cagaatccca acagccttga ggccgtcgaa gagatcttcc gcgccgcaca tacactcaag
      661 ggcatgtcgg ccacgatggg attcgagaaa gtcgcgcacg tgacgcacga gatggaaaat
      721 gtgctcgaca aactgcgcac caagcagatg cccgtcaccc ctgccgtcat cgatgtcgtt
      781 ttcgaaacct tcgatattct gcgcattctc gtcaatgaca gcatctcagc caccgactct
      841 caggtcgacc tcggcggaat ctcgcagaaa cttgccacga tcatctcttc gggtggtgca
      901 acgtctggcg gctcagcgcc agccggacag gaggaagcgc tctccgcggc cccggaaccc
      961 ggaacaacct cggcagaacc agcggccggt ggcagtgccg gcgatgaggg aggcgcacca
     1021 gatctgagcg aattcggact gagcgagttc gagatgagcg gtctggccga cgcacaacag
     1081 agcggcaaca tcccctatct cctggttgtc accctcgtag ccgactgtct tttaaaaggc
     1141 ccgcgcattt tcatggtcat gcgcagtctc gacaacctgc gctgcgaagt catcaagtcg
     1201 gtccccgata cgaaagacct tgaggcggaa cgatttgatc ggtcattcaa gatgatcatt
     1261 accatgaaga gcacggcgca agagttgaag gattcgatcg aatccatctc cgaaatcgag
     1321 cgcgccgacg tcattccagc cttcggaggc ggtggcgctg ctgtttcagt gccaccgcag
     1381 gctgccgcac cgctcccggt tgcggtcgcg gcaccagttc cggctccagt tgtggcaaca
     1441 ccccccactc cagccgcggc tccggtgact gcgcctgcga aagccgccgt gacggcaccc
     1501 gcacgcgcag cggctgctcc gatggctcct gccgccccac cgccggttgc cgcggcagga
     1561 ccggatgaac cacctgctgg ggccgtcccg cccggtggga aaccgccaga aagcgacggc
     1621 aagacgcagg aagccaagga atttcttgag ctgacccaac tcgtcagttt ccggatggcc
     1681 ggtgaaacct acgcactcga cattttcctg gtcgaaggca tcatcaacct ggtgcccatc
     1741 acccgtgttc ccaaagctcc gccccacatt gaaggggtca tcaacatgcg tggtgaaatt
     1801 g
//
LOCUS       DAORHD010001118         1801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85359_length_1801_cov_3.387171, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001118 DAORHD010000000
VERSION     DAORHD010001118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85359_length_1801_cov_3.387171"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1003
                     /locus_tag="PKO06_19210"
     CDS             <1..1003
                     /locus_tag="PKO06_19210"
                     /inference="COORDINATES: protein motif:HMM:NF019158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HNV71842.1"
                     /translation="SWEEFQLFFGPGRGGRDKRFFDMKAELCRQVWIPMAHRHLEFAE
                     LDSDDPDIELSFDRFFPTRLPGANVLDFFAEHMKKDFDCDITLPEIPWELSKTRINTV
                     IANVMETILRLFAEVIAQFDCDTLILGGKPSSLPIIREILLKLMPVAPDRIIGLKGYP
                     VGSWYPFSQKGGGISDPKTACVVGAAVWLFAEQLRNLDGLTLSTDGELIRKRECFIGT
                     FSPEVMTIDQQLFPTPEQQPASLEISKNALLGMRCIDASICPVNPIWELALNRDFVKS
                     AGALKVRLTQDPNQRECLTVAGIEDERGSKVDPRAVVLKLRSMVSDQYWLDTGCFDL"
     gene            1019..>1801
                     /locus_tag="PKO06_19215"
     CDS             1019..>1801
                     /locus_tag="PKO06_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71843.1"
                     /translation="MSDPKELFPFPVVGDDDAGANPSTNPSGSEALPGFPAAATAAEP
                     ASADALTAEEVKDLRRLLQEYRSGMLKPGSKKEEPAPLPLPVMPTISVPEKVPAAKQA
                     AELPLREQVLELLRQFDVTLPSEATVRQELPALLTEIAEPTALLTVWSAVCGVFPLRG
                     CLRHGTPDQRLFALIALGDRFCEHLTGIRWPGRQSLIKLMGRFLSEVSEGCSFLSMEQ
                     EPFQPTYHERVPGAASGNSLIRETRGYLVVRRQNNQVLRLGRV"
BASE COUNT          338 a          530 c          552 g          381 t
ORIGIN      
        1 ctcgtgggag gagtttcagc tgtttttcgg gccgggccgg ggtggccgcg acaagcggtt
       61 cttcgacatg aaagccgagt tgtgtcgaca ggtctggatc ccgatggcgc atcggcacct
      121 cgagttcgcc gaactcgact cggacgatcc cgacatcgaa ctgagcttcg atcgcttctt
      181 cccgactcgt ctgcctggcg cgaacgtgct cgacttcttt gccgaacaca tgaagaagga
      241 tttcgactgc gatatcacgc tgcccgagat tccgtgggaa ctctcgaaga cccgcataaa
      301 caccgtgatc gccaacgtca tggaaacgat tctgcgcctg tttgccgaag tgattgcgca
      361 gttcgattgt gacacgctga ttctcggcgg caagccgtcg tcgctgccga tcattcgcga
      421 aatcctcctg aaactcatgc cggtagcccc tgatcgcatc atcggcctga agggctaccc
      481 ggtcggcagc tggtatcctt tctcgcagaa agggggcggc atctccgacc cgaaaacggc
      541 ttgtgtggtc ggagccgcgg tctggctctt tgccgaacag cttcgcaatc tggacggact
      601 gacgttgagc accgacggcg agttgatccg caaacgcgag tgcttcatcg gcacgttctc
      661 gcctgaggtt atgaccatcg atcaacagct gtttcccact ccggagcagc agccggcgag
      721 cctcgagatt tcaaaaaacg ccctgctggg catgcgctgt atcgatgcct cgatctgtcc
      781 ggtgaatccg atctgggaac tggctctgaa tcgagacttt gtcaaatccg ccggagcgct
      841 gaaggtgcgc ctgacccagg acccgaatca gcgcgagtgt ctgaccgtgg cgggcatcga
      901 agacgagcgc ggctcgaaag tcgacccgcg agcggtcgtt ctcaagctgc gctcgatggt
      961 cagcgaccag tactggctcg ataccggctg cttcgatctg taaccaaggg agtcacgcgt
     1021 gagcgatccg aaagagttgt ttccgtttcc ggtcgtcggg gacgatgatg ccggggcgaa
     1081 tccgtcgacg aatccttccg gttccgaggc tctgccgggc ttcccggccg cggccacggc
     1141 tgccgaacct gcttcagccg atgcgcttac cgccgaagaa gtaaaagatc tgcgccgcct
     1201 gttacaggag taccggtcag gaatgctgaa accgggatca aaaaaggaag agccggctcc
     1261 gttgccgtta cccgtgatgc cgacgatcag cgtcccggag aaggtgccag ctgcgaaaca
     1321 ggcggcagag cttcccctgc gagagcaggt gctcgaattg ttgcgtcagt tcgatgtcac
     1381 cttgccctcg gaagcgaccg ttcgccagga gctgcctgcg ctgctgaccg aaattgccga
     1441 accgaccgcg ttgcttaccg tgtggagcgc tgtctgcggc gtgttccccc tgcggggatg
     1501 tctgcgccac ggtacacctg atcagcgact gttcgcgctg atcgctctgg gcgatcgttt
     1561 ctgtgaacat ctgaccggca tccgttggcc tggtcgtcag tccttgatta agctcatggg
     1621 gcggtttctg tccgaagtgt ccgaggggtg ttcatttctc agcatggaac aggagccgtt
     1681 tcaaccgacc tatcatgaac gggtgcccgg tgcggcatcc ggcaacagtc tgattcgtga
     1741 gacacgcggg tatctcgtag tgcggcggca gaacaatcag gttctgcggc tggggcgtgt
     1801 c
//
LOCUS       DAORHD010001119         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85640_length_1798_cov_2.755020, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001119 DAORHD010000000
VERSION     DAORHD010001119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85640_length_1798_cov_2.755020"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="PKO06_19220"
     CDS             <1..673
                     /locus_tag="PKO06_19220"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71844.1"
                     /translation="EAGSPQTLNLFSTTLKKEPNDFGLFCIEGLGNIGSPEAAQIIGT
                     ELKNPEWFIRKRLVVALGRCNCPGAVPFLIDALADESVQVRASAIEGLSQVGCHDIPL
                     MIRELEKTSSRERRIGLIRALGTMKDRAVLAPLIKTLADRTTLFVSLDVLGDLGFPEA
                     AESLIPFLRDSEWFNRLNAIEALGKLPLPHLKQMVEPCREDANDMVRHAVARILSRSE
                     EHPAA"
     gene            727..1395
                     /locus_tag="PKO06_19225"
     CDS             727..1395
                     /locus_tag="PKO06_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71845.1"
                     /translation="MKTRFHIVWVLAVFVGANLCLWADNPYRDNIEVQRVNTAIDNTL
                     NKAQATVSAVEGTVRQNATALDVRLQNGMNSLGKGLEDLAKAIDALTGGLKTAGNSVA
                     NSANGIVAGDNGCMVVPGSSGSTGAQSPSGTQNSGKELGQSVKDFFGSLGQLAKSFWG
                     WVKAAWQEITGPSKEEAPLTNAVNDIKTDWEKVKMEQAAKKVNQSAGKMFQAIGSFFK
                     TTFN"
     gene            complement(1441..>1798)
                     /locus_tag="PKO06_19230"
     CDS             complement(1441..>1798)
                     /locus_tag="PKO06_19230"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV71846.1"
                     /translation="VPVINLRRRLRLEERARVGDEQIIILHFEHEKVKVGFLVDSVHE
                     VLRLQQSAIEPSSRVSDVVDIEYLNGVGKIPIEKDKTAKQEKSEKQDNSEMKIIILLN
                     ASRIVFKGHELDIVDK"
BASE COUNT          406 a          462 c          528 g          402 t
ORIGIN      
        1 ggaagcaggc tcgccgcaga cactgaatct gttttcgacg actctgaaga aagaaccaaa
       61 cgatttcggt ctgttctgca tcgagggttt gggcaacatc ggttcgccag aggcggctca
      121 gatcattggc accgagttga agaatcccga atggttcatt cgcaaacgat tggtggtcgc
      181 tcttggccgc tgtaattgcc cgggggcggt tccctttctg attgatgctc tcgctgatga
      241 atccgtacag gtccgtgcga gtgccatcga gggcttgtcg caggtggggt gtcatgacat
      301 tcctctgatg atccgggaac tcgagaagac cagcagtcgc gaacgtcgca tcggcttgat
      361 tcgtgccctg ggaaccatga aagatcgtgc ggtactggca ccactgatca agaccctggc
      421 agaccgaacc acgctgttcg tatcactgga tgttctcggc gatctcgggt ttcctgaagc
      481 cgctgaatca ctgattccgt tcctgcgcga ttcagagtgg ttcaatcgct tgaacgccat
      541 cgaagcgctg ggaaaactgc cactgcctca tctgaagcag atggtggaac cctgccggga
      601 agacgccaac gacatggtgc gccatgccgt tgcccgcatt ttgtcgcgtt cggaagaaca
      661 cccggccgcc tgaaactttt tttcgggcgg cacgtagtac aagagaagaa aagaaggagg
      721 gcctgtatga aaacacgttt ccacattgtc tgggtgctgg ccgtgttcgt tggggcaaat
      781 ctttgcctct gggcggacaa tccctatcgg gacaacatcg aggtgcagag ggtcaacact
      841 gcgatcgaca atacgctcaa caaggctcag gcgacggttt cggcggtaga ggggacggtg
      901 cggcagaatg ccacggcact cgacgtgcgg ttgcagaacg gaatgaactc gctcggcaag
      961 gggctcgaag acctggccaa ggcgatcgat gccttgaccg gaggcctgaa aacggctggg
     1021 aactcggttg ccaactcggc gaatggcatt gttgccggag acaatggatg tatggtggtt
     1081 cccggaagtt cgggctctac cggtgcacaa tcaccatcag gaacgcaaaa cagcggcaaa
     1141 gaactgggac aatcggtcaa agactttttt ggctcgctcg ggcagctggc gaagtcgttc
     1201 tggggctggg tcaaggcggc ctggcaggag attaccggcc cttcgaagga agaggctccg
     1261 ctgaccaatg ccgtcaacga catcaagacc gactgggaaa aggtgaagat ggagcaggcg
     1321 gcgaaaaagg tgaatcagtc agctggcaag atgttccagg ccatcgggag ctttttcaaa
     1381 accacgttta actgagactc aaaacgctga ccggccggaa ctcatgctct ggccggtttt
     1441 ttatttatcc acgatatcga gttcgtgacc cttgaagaca atgcgcgagg cgttgaggag
     1501 gataatgatc ttcatctccg aattgtcttg tttttctgac ttttcctgtt tcgccgtctt
     1561 gtctttttcg atgggaattt tcccgactcc attcagatac tcgatgtcga ccacgtcgct
     1621 gacgcgtgag gatggttcaa tcgcactttg ctgcagacga agcacctcgt gcacgctgtc
     1681 aaccagaaag ccgaccttaa ccttttcgtg ttcgaagtgc aggatgatga tctgttcatc
     1741 gccgacacgg gcgcgttctt cgagacgcag acggcgcctc agatttatca cgggaaca
//
LOCUS       DAORHD010001120         1796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85785_length_1796_cov_2.874210, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001120 DAORHD010000000
VERSION     DAORHD010001120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85785_length_1796_cov_2.874210"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PKO06_19235"
     CDS             complement(<1..548)
                     /locus_tag="PKO06_19235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71847.1"
                     /translation="MKRLCQIAGILFLMSLSVVLEAGTADRLRYLGSSCFAMPTGYVR
                     NEGGYFSDDNHSMGWFSYPVLGKFLEFSGLRHMQGDAKDKNVLNLKVNFLEEDEYLPN
                     LVWGAADVREELGSKIFYVAGSKTFETYGLTIHGGVIKDPITTEKRGYIAAEKMIMPL
                     VTLAGERFDGKTVLGLKLRPYPG"
     gene            complement(545..1621)
                     /gene="lpxD"
                     /locus_tag="PKO06_19240"
     CDS             complement(545..1621)
                     /gene="lpxD"
                     /locus_tag="PKO06_19240"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNV71848.1"
                     /translation="MKQAHARAKPHMMTLGALAEHLGGRLEGGNPDLSISGIAPITEA
                     LEGMVTFLTDAKKVAEHREELSASKAVAVIAAEAAGPLPLPVIRVPHAYAGLVKALQL
                     FDPSRQPLPGLHPTSFVHEDAQVHPTASVGPLAVIEAGAVVGAKTRIDAQVNLGPNVR
                     VGERCHLHARVVVRERCIIGDRCILQAGCVVGSDGFGYARTPEGQIKIPHIGIVQVDD
                     DVELGANVTIDRGTMGVTHIGAGTKIDNLVHVAHNCVIGRQCTIVAQVGLSGSTILED
                     QVTLAGQVGTVGHVRVGAGTTVAARGVVTQDVEPQSFVSGFPLKPHQEERRIMVALRR
                     LPELVRLMNKLKKQHPELSEEEKS"
BASE COUNT          405 a          562 c          449 g          380 t
ORIGIN      
        1 cctggatatg gtctgagttt taacccgaga accgtcttgc cgtcaaaccg ttctccagcc
       61 agcgtgacaa gcggcatgat catcttttcc gccgcaatgt atccacgctt ctctgtggta
      121 atggggtctt tgatcacccc tccatgaatc gtcagaccat aggtttcaaa agtttttgac
      181 ccggcgacat agaaaatctt cgaccccagt tcttcgcgca catccgcagc gccccagaca
      241 agattcggca gatactcgtc ttcttcaaga aaattcactt tcagattcag aacgttttta
      301 tctttcgcgt caccctgcat atgtcgcaaa ccggaaaact ccagaaattt tccgagaacg
      361 ggatagctga accaccccat ggaatgattg tcatcactga aatagccccc ttcattgcgc
      421 acatacccgg tcggcatggc aaaacaggat gagcccagat agcgcaggcg atccgcagtc
      481 cccgcttcca acaccaccga caaactcata aggaacagga ttcctgcaat ctggcagagc
      541 cgtttcatga cttctcctcc tcggacaatt ccgggtgttg tttcttcagt ttgttcatca
      601 gccgtaccag ttcgggcagt ctgcgcaacg cgaccatgat ccgccgttcc tcttgatggg
      661 gcttcagcgg gaatccggaa acaaagcttt gcggttcaac atcctgggtg acgacgccgc
      721 gagcggcaac cgtcgtgcca gctccgacac gtacgtgccc caccgtaccg acctggcctg
      781 cgagagtgac ctgatcctca agaatggtac tgcccgacag cccgacctgc gccacaatcg
      841 tgcactgacg gccgataaca cagttgtggg cgacatgaac caggttatcg atcttcgttc
      901 cggcaccaat gtgcgtcact cccatggttc cccggtcaat ggtcacgttc gctccaagtt
      961 cgacatcatc gtcgacttgt acaattccga tatgcggaat cttgatctgt ccttccggag
     1021 tccgggcgta tccaaacccg tcgctaccca caacacaacc ggcctgcaga atgcaacggt
     1081 cgccgatgat acatcgttcg cgcacgacga cgcgggcatg cagatgacag cgctcgccaa
     1141 cccggacatt cggcccaaga ttcacctggg catcaatgcg tgtcttcgct ccaacaacag
     1201 cgccggcctc gatcaccgcc agcggcccga cagaagcggt gggatgaacc tgagcgtctt
     1261 catggacaaa agaggtcgga tgaaggcccg gcagaggctg acgactcggg tcaaagagtt
     1321 gcagggcctt caccaacccg gcataggcat ggggcacccg aatgaccggc aagggcaacg
     1381 gcccggccgc ttcagcggca atcaccgcca cggctttgct cgccgacaat tcctcacgat
     1441 gttcggccac cttcttggcg tcggtcagaa acgtgaccat tccctcgagt gcttccgtaa
     1501 tcggagcaat gccggaaatc gacagatcag gattgccgcc ctcaagccgt cccccgaggt
     1561 gttccgcgag ggctccgaga gtcatcatgt gcggtttcgc ccgagcgtgc gcctgtttca
     1621 tgcagtgact aatcctcgtt ggcagatcag attcagaaag cctcatgaga aacgcgtttt
     1681 gcaccagttc tcatcaggag cggcattcct cgcggtgaaa aacagggggc acggaaaatc
     1741 cgcgccccct gctgatgtag caaatcctcc gacttacttg ccaccctttt tggccg
//
LOCUS       DAORHD010001121         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85824_length_1795_cov_4.969540, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001121 DAORHD010000000
VERSION     DAORHD010001121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85824_length_1795_cov_4.969540"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PKO06_19245"
     CDS             complement(<1..324)
                     /locus_tag="PKO06_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71849.1"
                     /translation="MTDQLASSIAVRDSGPGLLERVILCLLFLLPATGLDFLLRTWQT
                     STLAESQNEVREQAVELMTNFDRHLQPERICSNTLQRFFQRRQHLELTPVVAQGLHES
                     FARHFP"
     gene            complement(382..>1795)
                     /locus_tag="PKO06_19250"
     CDS             complement(382..>1795)
                     /locus_tag="PKO06_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71850.1"
                     /translation="TRFPEYADRHSQNNQAVEMIRLKIRDLKLLVRGIGVGFLILSLI
                     LFLLLRVIYGSGESRFQFNRWLYRLFNKDNACRQVQTLVHSFTPIQRKVFEMSFAASK
                     GNPDGSFDLDILKWYKMVFPTDDEVSERKRANIAIAFYELCEEFFFFQMTRANQPVFR
                     TYCPIIPFEDQVTKEFVRDLSGREVEVYKAGRFRFSPPLYQDFLRHRYPRLPHPVTYA
                     IDDKKHPYAFRLYILLWELWYDQNQREDKRGMTSISINLNDLLNRVCVEMVTSPETNL
                     LEQLHLDIAHLKDFGAIRTCVIEEHGEKVAFTWPKEAELFVYDATINQYRQNSHLLVN
                     KKYSFEMVPWDSLEKLKPTKRRCAYLDEFDTPCTRPAIQGSPYCKRHAKVLEHDAPRL
                     FYGKVKFLPPGNADTKTETESSNQDSDSKSVSEAEIVSAPEASTPAQKPTQEKPPEVI
                     ITPPPAAPDADPPMSSSPKP"
BASE COUNT          402 a          421 c          554 g          418 t
ORIGIN      
        1 gggaaagtgt cgcgcaaagc tttcgtgaag cccttgtgcc actacaggag tgagttcgag
       61 gtgttggcga cgttggaaga atcgctggag tgtattcgag cagatccgct cgggctgcag
      121 atgtcgatcg aagttcgtca tcaactccac cgcttgttcg cgcacctcat tctgagactc
      181 ggccagggtt gaggtctgcc aggtgcgcaa caggaagtcg agtccggtgg ccggcagtaa
      241 aaacagcagg cacagaatga ctcgctcgag aagcccggga ccggagtctc gaacggcgat
      301 cgatgaggcg agctgatcag tcatggaatg tcgctgtcgg gttctgattc agcgattgga
      361 ggacaaaaac aatgatgatt gtcagggctt gggagacgac gacattggcg ggtcagcgtc
      421 tggtgcggca ggtggcggag tgatgatgac ctcggggggc ttttcctgtg tcggcttctg
      481 ggcgggtgtc gacgcttcag gggcagaaac gatttcggcc tcgctcactg atttgctgtc
      541 tgagtcctgg ttggatgatt cggtctccgt ttttgtgtcg gcattcccgg gggggagaaa
      601 tttgaccttg ccgtagaaca gtcgcggggc atcgtgttcg agaaccttcg cgtgtcgttt
      661 gcagtacgga ctgccctgaa tggcggggcg agtgcaggga gtatcgaatt cgtcgaggta
      721 ggcgcatcgc cgtttcgtgg gcttcagctt ttcgagggaa tcccagggga ccatttcaaa
      781 agaatacttc ttgttgacca gcaggtgaga gttctgacgg tactggttga tggtggcatc
      841 atacacgaac agttccgctt ctttgggcca ggtaaaggcg actttctcac cgtgctcttc
      901 gatcacacag gtgcgaatcg ccccaaaatc cttgagatgc gcaatgtcga gatgcaactg
      961 ctcgagcaga ttggtctcgg gtgacgtgac catctcgacg cagacacgat tgagcagatc
     1021 attgaggttg atcgagatcg aggtcatgcc acgcttgtct tcgcgttgat tctgatcgta
     1081 ccagagctcc cagagcagaa tgtagagccg aaaagcatac gggtgcttct tgtcatcgat
     1141 cgcgtaggtc accgggtgcg gcaaacgcgg ataccgatgt cgcagaaagt cctggtagag
     1201 tggtggcgaa aaccggaaac gaccagcttt gtagacttca acttctcggc ctgacagatc
     1261 gcgaacgaac tcttttgtca cctgatcttc gaacggaatg atgggacaat aggtgcgaaa
     1321 gaccggttgg ttggcccgtg tcatctggaa gaagaagaat tcctcgcaga gttcgtaaaa
     1381 tgcgatggcg atgttggcgc gttttcgctc cgagacctcg tcgtcggtcg gaaagaccat
     1441 tttgtaccac ttgagaatgt cgaggtcgaa gctaccgtcg gggttccctt ttgaagcggc
     1501 aaagctcatc tcgaagacct tgcgttgaat gggggtaaag gaatgaacca gcgtctgcac
     1561 ttgtcggcac gcgttgtcct tgttgaacaa tcggtacaac caccgattga attgaaaacg
     1621 actttcccca ctgccgtaga tgacgcgcag aaggagaaaa aggatcagtg aaaggataag
     1681 gaatccgacg ccgatccccc gaacgagcag tttgagatcg cgtatcttga gtcggatcat
     1741 ctcgaccgct tgattgttct gcgaatgccg gtctgcatat tcgggaaaac gagta
//
LOCUS       DAORHD010001122         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85986_length_1793_cov_3.574799, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001122 DAORHD010000000
VERSION     DAORHD010001122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85986_length_1793_cov_3.574799"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PKO06_19255"
     CDS             <1..789
                     /locus_tag="PKO06_19255"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV71851.1"
                     /translation="QELLQAEGATLYLVDSIEKLMISQVILSDRVEEIVLNIDNTSIA
                     GFTALNRQSLNIPDAYGDLSSIHPQLKFNRAIDQAHNFRTRNILTFPIIIKEDLIGVF
                     QIINRKDGTFGDNDKLLLKNFAVLSGIAILNARLVERVVDEQEISRGIIEYTSDEVYI
                     QHRDGRLLHCNRKAQEKIPEGQSLQSIKNKPLLEVFPALSGLQREVQKVIENNLDKAF
                     SGGKMPYVILSAKNSRGMVEKVIIIIKVHSEEHETGIPMDSQRY"
     gene            816..>1793
                     /gene="speY"
                     /locus_tag="PKO06_19260"
     CDS             816..>1793
                     /gene="speY"
                     /locus_tag="PKO06_19260"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HNV71852.1"
                     /translation="MAATKQHKSRYLSGTKINPKPVKKGLSVDALIDGAFMSYNGGRL
                     REACRLFSEKMLKPDVTIGMSLAGALTPAGLGQSVIVPLIKAGFVDWITSTGANLYHD
                     THFGIGEELYAGSPFYDDIALRKDGVVRIYDVVFDYSVLLNTDSFYREIMTSSVFQKP
                     MGTAEFHHHVGKFIFEREKQLGIKNTSLLSAAYQAGVPVFTSSPGDSSIGMNIAALAL
                     TGSKLVLDPNRDVNETAAIVLNAKRTGGKSAVFICGGGSPKNFMLQTEPQIQEVLGIK
                     EEGHDYFLQLTDARPDTGGLSGATPAEAVSWGKVRPDSLPDTVVCYVDST"
BASE COUNT          437 a          512 c          478 g          366 t
ORIGIN      
        1 caggagctcc tgcaggcgga aggagcaacg ctgtatctcg tcgactcgat cgagaaactg
       61 atgatttcgc aggtcattct cagcgatcgc gtcgaagaga tcgtgctcaa catcgataac
      121 acgtcaatcg ccgggttcac tgctctgaat cgccagagcc tgaacatacc cgacgcctac
      181 ggtgatctgt cctcgattca tccccagttg aaattcaacc gtgccatcga tcaggcgcac
      241 aacttccgca cccgaaacat tctgaccttt ccgatcatca ttaaagagga tctgatcggc
      301 gtgtttcaga tcatcaatcg caaagacggc acgtttggcg acaacgacaa actgcttttg
      361 aagaacttcg ccgtgctttc cggcatcgcg attttgaacg cccgtctggt cgagcgtgtc
      421 gtcgatgaac aggagatctc gcgcggcatc atcgaataca cttccgacga agtgtacatt
      481 cagcatcgag acgggcgcct gctgcactgc aatcgcaaag cccaggagaa aatccccgag
      541 ggccaatcgc tgcagagtat caagaacaag ccactgctcg aggttttccc ggccctgtcc
      601 ggattacaga gagaagtgca gaaggtcatc gaaaacaatc tcgacaaggc tttttcgggc
      661 ggcaaaatgc cctatgtgat tttatcggcc aagaattctc gcggcatggt cgagaaggtc
      721 atcatcatca tcaaggtcca cagcgaagag catgaaaccg gaatccccat ggattctcaa
      781 cgttactgaa gacgtcatac tcaggagtac aagtcatggc agcaacaaaa cagcataaaa
      841 gccgatactt aagcggaacg aaaataaacc ccaaacccgt caaaaaagga ttgtcggtcg
      901 acgctctgat cgacggcgct ttcatgtctt acaacggtgg gcgcttacgc gaagcctgcc
      961 ggctcttttc cgaaaagatg ctcaagccgg acgtcacgat cggcatgagt ctggccgggg
     1021 ctttgacgcc agccggcctc ggacaatcgg tgatcgtccc attgatcaag gcgggattcg
     1081 ttgactggat cacctcaacc ggcgccaatc tctatcacga tacccacttc ggcatcggtg
     1141 aagagctgta tgccgggtcg ccattctacg atgacatcgc actgcgcaaa gacggtgtcg
     1201 tgcgtatcta cgacgtggtc ttcgattaca gcgtgctgct gaataccgac agtttctatc
     1261 gtgaaatcat gaccagttcg gtgtttcaga agcccatggg aacggcggag tttcaccacc
     1321 acgtcggtaa gttcatcttt gagcgcgaga agcagctggg aatcaagaac accagcctgc
     1381 tcagtgctgc ctaccaggcc ggcgtgccgg tcttcacttc gagccccggc gacagttcga
     1441 tcggcatgaa catcgccgcc ctcgccctga ccggttcgaa gctggtgctc gacccgaacc
     1501 gtgacgtgaa tgaaacggcc gccatcgtgc tcaacgccaa gcgtaccggc ggcaagagcg
     1561 cggtcttcat ctgcggcggc ggttccccga aaaacttcat gctgcagacc gaaccgcaga
     1621 ttcaagaggt gctcggaatc aaggaagagg ggcatgacta ctttctgcaa ttgaccgacg
     1681 cacgtccgga tacgggtggc ctgtccgggg caacgccagc tgaagccgtc agctggggca
     1741 aggtgcggcc tgacagtctg cccgacacgg tggtctgtta cgtcgattcg acc
//
LOCUS       DAORHD010001123         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_85999_length_1793_cov_3.286536, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001123 DAORHD010000000
VERSION     DAORHD010001123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85999_length_1793_cov_3.286536"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..709)
                     /locus_tag="PKO06_19265"
     CDS             complement(158..709)
                     /locus_tag="PKO06_19265"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HNV71853.1"
                     /translation="MATDSFKITDRRSAYTDKDEPNEAPQKPLAPTPPEPPPQQPNPT
                     ISPPPEADEEFGDSSEAMDERFDEGPLPDELPDDQLDEPVEPRQARGSPGRATPFSMR
                     PIELTHSLLGLLVQKAYVSLGLMPNPETGRMTRDLDDAGESIELLEVLVKHFSGKWQV
                     PGMEAELQNQLSNLRLQYARLKG"
     gene            960..>1793
                     /locus_tag="PKO06_19270"
     CDS             960..>1793
                     /locus_tag="PKO06_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71854.1"
                     /translation="MVRSADEKRLHRLRPFFLMVVGLLICLAGVVHAAPPPTIASVAS
                     IPVVIGGTTYASTSGNITFVGTADPSAFVRLYNGTTEVGASVVDALGSWSIVVPGVAA
                     GFRNYRAYSISYPTLVQQSATAAMCFDVAVPTGVTFNDSVLANNDPNYDFTVDNKIYY
                     NVAAADALSGVASIHLQVATNSGFLTPVFDGLIATTSPYCFPGATASNTYFARIRAFD
                     RAGNVSGWTAAATMTRVVQESMVLPSVPMLTTVGGKPANSGATLYFSAATLSVKGNSD
                     PF"
BASE COUNT          353 a          476 c          535 g          429 t
ORIGIN      
        1 cagatcctgt tcgtcgacca gatacagaaa agttccgatg atcatttccc gccgatcggc
       61 cggaatgtgc gaaaaattca catagcgcag aaagcggttg atgtacttca gcagagtttc
      121 ttcgacatgc agttgaaaat tcgattttcc catgagctta tcctttcagg cgggcatact
      181 gcaatcgcag attcgagagc tggttctgca gttccgcttc catgccggga acctgccatt
      241 tgccggaaaa atgcttgacc agcacttcga gcaactcgat tgattcgccg gcgtcgtcaa
      301 ggtcgcgcgt cattcggccg gtctcggggt ttggcatcag ccccagactc acatacgctt
      361 tctggaccag aagcccgagc agcgaatggg tgagctcgat cggacgcatc gagaacgggg
      421 tggctcgacc ggggctaccg cgtgcctgac gtggctcgac cggttcatcc agctggtcgt
      481 cagggagctc gtcgggcagt ggaccttcat cgaagcgctc gtccatcgcc tctgacgaat
      541 cgccgaactc ttcgtctgcc tcgggtgggg gcgagatcgt cgggttgggc tgttgtggcg
      601 ggggttccgg cggagttggc gctaacggct tttggggagc ttcgttcggt tcgtctttgt
      661 cggtgtatgc cgaccgtcgg tcagtgatct tgaaagaatc tgttgccatg cacatgcctc
      721 tcgtggtgtt cagcttactc tagcatggat tttcatgcgg cgacaagcag ccgaatcagc
      781 gtggcgataa gggtgctttc cagcatcagc ggcaactggt ccgcgagtcc ggggcatatt
      841 gagcgaagac gtgggatgac gaagaaactg gccgacgagt gaccccctga ttctcgagag
      901 gtttttctgt tcctcaaatg ggattcgtac aggtatgatg gagaaggagg agagtgttca
      961 tggttcggtc agctgacgaa aagcgacttc acagacttcg accatttttc ctgatggttg
     1021 tcgggctgtt gatctgcctt gctggcgttg tgcatgcagc gccaccacca accattgcgt
     1081 ctgtggcgag tattccggtg gtcatcggtg gaacgaccta tgcctcgacg agcggaaaca
     1141 tcaccttcgt gggaacggct gatccatctg catttgtcag gctctacaac ggaaccaccg
     1201 aagtcggagc gagcgttgtt gatgctctcg ggagctggag tatcgtggtt cccggtgtag
     1261 ctgcgggctt tcgtaactat cgggcctatt ccatttccta tccgacgctg gttcagcaga
     1321 gtgccacggc tgcgatgtgt ttcgatgtcg cggttccgac cggtgtgacg ttcaacgaca
     1381 gcgtgctcgc gaacaatgat cccaactatg atttcacggt cgacaacaag atctattaca
     1441 acgtggcggc tgcagatgcg ctgagtggcg ttgcctccat tcacctgcag gtagcgacca
     1501 actcaggatt tttaacgccg gtattcgacg ggctcatcgc gaccacgtcg ccgtactgct
     1561 tccccggggc aactgccagt aacacctact ttgcccgcat tcgcgccttc gatcgtgctg
     1621 gaaacgtgag tggctggact gccgccgcaa ctatgactcg ggttgtacaa gaatcgatgg
     1681 tgctgcccag cgtgccgatg ctgacgacgg tgggtggaaa accagcaaac tcgggggcga
     1741 cgctttactt cagtgcggca actctttcgg tgaagggaaa cagtgatcct ttc
//
LOCUS       DAORHD010001124         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86070_length_1792_cov_3.063903, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001124 DAORHD010000000
VERSION     DAORHD010001124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86070_length_1792_cov_3.063903"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKO06_19275"
     CDS             <1..337
                     /locus_tag="PKO06_19275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV71855.1"
                     /translation="AVVGPARVQAFARGFFIEQGVLRAMVKPGVVPFTGRTPHGEIIV
                     LGTIFVCRVGEHQTEVQVEQGKVRIVSMRGPERVLVAGQKASMLYSAHAGTPGEIREN
                     PAVESDPSQ"
     gene            276..>1792
                     /locus_tag="PKO06_19280"
     CDS             276..>1792
                     /locus_tag="PKO06_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71856.1"
                     /translation="MPAHPEKSAKTLRSRAILPNKLNRHAVTGGILLPLLMGFAVIVG
                     LLALLLSRSASQAQYYTFRHDQDRWLRRWAEMAVEEAFIRCEAALKKPDHPARRCLLD
                     DHLTEYLLELPLTTAEMARRSGLGVGKVQATAALRPLDSRKSDSNGYRFFAGELVGTF
                     EIQATISFSDERRQGLSLRRHFPYRVTSLAHPRTGLESREGYYGAFLQDYVLFVRDGL
                     AEFRQTRGSSLHAPESAQRRIRVELASLPEEQRGKIFFGGTRDPEAYSSAKPGETPLG
                     NYVFINVPASWSFLLPEAGKVLEIPLTLSDCLSLVPRFAPFRDGLSGLQGTVQIRPIP
                     VVGSETASEKMAYNPFPGLEGAERNLDPPCLVFDSAILRDPRMAEQVFEGAIRQRFLC
                     ESRLTLDFSRLGTPLPASIDGISGQEGRLVFEFGRHGAQGARQPERELWQRLMAWNQD
                     RLGPLGSAGNAEFLLCAGQRWHDRQPWKAFHKPRLYNSRGELIDARESGGGGLRP"
BASE COUNT          335 a          490 c          564 g          403 t
ORIGIN      
        1 ggccgttgtc ggcccggccc gcgttcaggc gtttgcgcgt ggttttttca tcgagcaggg
       61 cgtgttgcgg gccatggtca aacccggggt ggtgccgttc acggggcgaa ctcctcacgg
      121 cgagatcatc gtgctgggca cgatcttcgt ctgccgagtc ggtgagcatc agaccgaggt
      181 gcaggtcgaa cagggaaaag tgagaattgt atcgatgcgc ggcccggagc gcgttctggt
      241 tgccggtcag aaggcgagca tgctatattc cgcgcatgcc ggcacacccg gagaaatccg
      301 cgaaaaccct gcggtcgaga gcgatccttc ccaataagct caaccgtcac gccgtcaccg
      361 gcggaattct cttgccgctg ctgatgggtt tcgcggtcat tgtcgggctc ctggcgcttc
      421 tcctgtcacg ctccgcctct caggcacagt attacacgtt tcgacacgat caggaccggt
      481 ggttgcggcg ctgggcggag atggccgtcg aagaggcatt catccgctgc gaagccgcgc
      541 tgaaaaaacc tgaccacccg gctcgtcgct gtctgctcga tgatcacctg accgagtatc
      601 tgcttgaatt gccgttgacg acggcggaaa tggcgcgacg aagcggattg ggtgttggaa
      661 aagtacaagc tactgcagcg ttacgtccgc ttgattcaag aaagtctgac agtaacggat
      721 atcgtttctt tgccggcgag ttggttggaa cctttgaaat tcaagcgact atctcgtttt
      781 ctgacgagcg acggcaggga ctctcattgc gaaggcactt tccctatcgt gtcacgtctc
      841 tggcgcaccc tcgcacgggg cttgaatcgc gagaagggta ttacggagcg tttctgcagg
      901 attatgtcct gtttgtccgc gatggtctgg cagagtttcg gcagacgcgc ggatcgagtc
      961 tgcatgctcc agaatcagca cagaggcgta ttcgggtcga gttggcctct ctaccggagg
     1021 agcagcgcgg taagatcttt tttggtggaa cccgagatcc agaggcttac tcctcggcca
     1081 agcccggcga aacaccgctg ggaaactatg tgtttatcaa tgttcccgcc tcctggagct
     1141 ttctcctgcc agaggcgggg aaggtgttgg agattccgct caccttgagt gactgcctgt
     1201 cactcgtgcc gcgctttgcc ccctttcgcg acggattgag cggtctgcag ggaacggtgc
     1261 agatacgtcc gattcccgtt gtcggctcgg aaaccgcatc ggaaaagatg gcctacaacc
     1321 cgtttcccgg gctcgagggc gcggaacgga atcttgatcc gccgtgtctg gtgtttgact
     1381 ccgcgattct gcgggaccca cggatggcgg aacaggtctt cgaaggggcg attcgacagc
     1441 gatttttatg tgagtcgcgc ctcacgctgg atttttcgcg tcttggaacg ccgctgccag
     1501 catcaattga cgggatctct ggccaagagg gtcgcctggt attcgagttc ggacgtcacg
     1561 gagcacaagg tgccagacag cctgaacgtg aactctggca acgcctgatg gcctggaatc
     1621 aggatcgcct ggggcctttg ggaagcgccg gaaatgccga gtttctgctg tgtgccggtc
     1681 agcgttggca tgatcgacag ccctggaagg cttttcacaa accacgactg tacaactcgc
     1741 gcggtgaact gattgatgcg cgcgaaagtg gtggtggggg gcttcggccc tt
//
LOCUS       DAORHD010001125         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86074_length_1792_cov_2.926885, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001125 DAORHD010000000
VERSION     DAORHD010001125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86074_length_1792_cov_2.926885"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PKO06_19285"
     CDS             <1..1262
                     /locus_tag="PKO06_19285"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNV71857.1"
                     /translation="TQLYKAAPIDVASDDPRELLREALGKGLKALAGRSVKTVAVLLP
                     ALDLTILAGLFEVPVLMSFNPAPYKTKTDDAKPFPITEVFFCTATAGTDKKLNALCRE
                     AQILAEAVNLARKLANEPANLLTPKQFVQEAGLLQKLGIEVRTLSPALLKKMGGVQAV
                     GAGSENSPQFLIMELNPGKQKPIVLIGKGVTFDSGGISIKPADGMAEMKNDMGGGAAV
                     VGAMAALARLGYKKHVVGLVPLVENMPDGRALKPSDVITMFDGQTVEVLNTDAEGRLI
                     LADAVAYAATLNPEFTVDIATLTGAVIIALGNRFTGTFTTDEALYRRLETASQASYDL
                     IWRLPLHKTFTEEMKSTIADWRNLGGSRKAGASLGAAFIGAFAKGPWAHLDIGGSVMV
                     EKANAYMPAGPSGAGVRLLTRLVLSGR"
     gene            1332..>1792
                     /locus_tag="PKO06_19290"
     CDS             1332..>1792
                     /locus_tag="PKO06_19290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71858.1"
                     /translation="MAFAWKKLFLFALFVAGLVSILPGCTQTNSLLNGTNPLGTDDEQ
                     LLATDTLVTGRNLAPLVNLSVTNTAVGFGGEITLTAEAIDPDGDALTFSWQASEGTLV
                     TPLQNRATWKAPSRMGAITITCSADDRRGGVTTASTTVSVVGGRKYLLDLSL"
BASE COUNT          380 a          587 c          482 g          343 t
ORIGIN      
        1 aaactcagct ctacaaagcg gcccccattg atgtcgcatc cgatgatccg cgcgagttgc
       61 tgcgcgaagc gctggggaaa ggtctgaagg cactggcggg acgttcggtc aagaccgtag
      121 ccgtgctcct gcccgcgctc gatctgacaa ttcttgccgg tctcttcgaa gttcccgtgc
      181 tgatgagctt caatcctgca ccgtacaaga ccaaaaccga cgacgccaag ccgttcccca
      241 tcaccgaggt gtttttctgc accgcgaccg ctggcaccga caagaagctg aacgccctgt
      301 gccgtgaagc gcagatactc gccgaggcag tgaacctcgc ccggaaactg gctaatgaac
      361 ccgcaaacct gctgaccccg aaacagtttg tccaggaagc gggccttttg cagaagctcg
      421 gcattgaagt tcgcacgctt tcccccgcgt tgctgaagaa gatgggcgga gtgcaggccg
      481 tcggcgcggg gagcgaaaac tccccgcagt ttctgatcat ggagctgaac ccaggcaagc
      541 agaaaccgat cgttctcatc ggcaaggggg tcaccttcga ttccggcggg atcagcatca
      601 aaccggcaga cggcatggcc gagatgaaga acgacatggg cggtggcgcc gccgtcgtcg
      661 gcgctatggc agcattagcg cgcctcgggt ataaaaagca cgtcgtcggg ctcgtaccgt
      721 tggtcgaaaa catgccggac ggtcgcgcgc tcaaacccag tgacgtcatc accatgttcg
      781 acgggcagac tgtcgaagtg ctgaacacag acgccgaagg ccgtttgatt ctcgccgacg
      841 cggtcgccta tgccgccact ctcaaccccg aattcaccgt cgacatcgcc accctcaccg
      901 gcgccgtcat catcgcactt ggcaaccggt tcacgggaac cttcaccacg gacgaagccc
      961 tgtatcgtcg tcttgaaacc gcttctcagg cttcatacga cctcatctgg cgattgccgt
     1021 tgcacaagac ctttaccgaa gagatgaaaa gcaccatcgc cgactggcgc aacctcggcg
     1081 gcagccgcaa agccggcgcc agtctcggag ccgccttcat cggcgctttt gccaaaggac
     1141 cctgggctca tctcgacatt gggggctcgg tcatggtcga aaaagccaac gcctacatgc
     1201 cagctggtcc ttccggcgca ggtgtacgcc tgctcacccg gcttgtgctc tccgggcgct
     1261 gattcgacca cgactcaact ttccaggcga atcctccgat aacttcctga gacacctcag
     1321 aggagtcacg tatggcgttt gcctggaaaa aactcttcct gttcgctctg ttcgttgccg
     1381 gtctggtcag cattctcccc ggatgcacac agaccaattc acttctgaac ggaaccaacc
     1441 cgctcggcac cgatgacgaa caactgctcg cgactgacac cctcgttacc ggtcgcaatc
     1501 tcgctccatt ggtcaatctt tccgtcacca acaccgcagt cgggtttggt ggagagatca
     1561 ccctgacggc tgaagccatc gatccggacg gcgacgctct gacattctcc tggcaggcat
     1621 cagaaggcac tctcgtaact ccactccaaa atcgggccac ctggaaagcc ccttcgcgaa
     1681 tgggtgccat caccatcacc tgctccgccg atgatcggcg aggcggcgtc accaccgcga
     1741 gcaccaccgt cagcgtcgtc ggtgggcgta aatacctact ggatctatct ct
//
LOCUS       DAORHD010001126         1792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86099_length_1792_cov_2.069085, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001126 DAORHD010000000
VERSION     DAORHD010001126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86099_length_1792_cov_2.069085"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="PKO06_19295"
     CDS             <1..126
                     /locus_tag="PKO06_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020160895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNV71859.1"
                     /translation="LRTWKIAHAIPKKWLVSDLNANESSVVIETMELTYRYFTVQ"
     gene            215..394
                     /locus_tag="PKO06_19300"
     CDS             215..394
                     /locus_tag="PKO06_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HNV71860.1"
                     /translation="MPIEIKELHISFAVFCLNKTAGQTTPVVDAQGVGGADREALVAE
                     CVEQVLQILRNKSER"
     gene            394..1098
                     /locus_tag="PKO06_19305"
     CDS             394..1098
                     /locus_tag="PKO06_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV71861.1"
                     /translation="MAESGKLEKMLILAFSDSRKAESGGLAEADDSFEALINPESYTQ
                     EYKLKFSESGQGQGTSGKQLKYEYTQPEEMTFEFLFDNTGIIDGKPRDSVADDLKLFK
                     QVLIEYKGDSHEPRHFKLVWGQNSIFKGRVTEVSITYKMFKSDGTPIRAVAKAKFRSS
                     IEEQKRAARENKSSPDLTHVRRVKRGDTLPQLCYRIYGDPRYYIQVAEFNALENFRVL
                     RPGSDLTFPPLEKATR"
     gene            1100..>1792
                     /locus_tag="PKO06_19310"
     CDS             1100..>1792
                     /locus_tag="PKO06_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HNV71862.1"
                     /translation="MSNESTIPTPATPDVCTLTVLVEGQEISSEFHVLSLSISRELNR
                     IPAATLQIQDGDAAKGTFAVSDTDSFVPGKGIEIQLGYRSNNETVFKGIVVKQAIRIR
                     KGGNVLTVECRHDAVRMTSGARSRYFVDQKDSDVMEEVIGFYGLQKSVESTLPDLKEV
                     VQYDTSDWDFLLCRAEANGQVVLVEDDKVSVGKPLTAATAVLKIAYGATVLELDAEID
                     ARWQSKGITASSW"
BASE COUNT          448 a          442 c          548 g          354 t
ORIGIN      
        1 ttgcggacgt ggaagattgc ccatgcgatc cccaagaagt ggctggtgag tgacctgaat
       61 gccaacgaaa gcagcgtcgt gattgaaacg atggaactga cctaccgcta tttcactgtc
      121 caatgagtcc atccgcttgt ggcgaggttg acgcgcggcg taatggctta ctcaaccgac
      181 ctgttctgcc cattttttgg aaagccggga gctgatgccc atcgaaataa aggagttgca
      241 tatctcgttt gccgtcttct gcttgaacaa aaccgcaggg cagaccacgc cggtagttga
      301 tgcacaaggc gtcggcgggg ccgaccggga ggccctggtg gccgagtgtg tggaacaggt
      361 gttgcagatc ctcaggaaca agagcgaaag ataatggcgg aatccggcaa acttgaaaag
      421 atgctcattc tggcgttttc cgattcccgg aaggcggaga gcggcggtct ggccgaagcg
      481 gacgattctt tcgaagcgct gatcaatccc gagagttaca cgcaggagta caaactcaaa
      541 ttctccgaat ccgggcaagg ccagggtacc agcggcaagc agttgaagta cgagtatacc
      601 cagcccgagg agatgacttt cgagtttctc ttcgacaaca ccggaatcat tgacggaaaa
      661 ccacgcgatt cggttgccga cgatttgaag ctgttcaagc aggtgttgat agaatacaag
      721 ggagattcgc acgaaccacg ccacttcaaa ctggtgtggg gacaaaactc cattttcaag
      781 gggcgtgtca ccgaagtcag catcacctac aagatgttca agagtgacgg aacgcccatt
      841 cgtgcggttg ccaaagcgaa gttcaggagc agtatcgagg agcaaaagcg tgcggccagg
      901 gagaacaaga gttcaccgga cctgacacac gttcgccggg tcaagagagg ggatacactg
      961 ccgcaactgt gttatcgcat ctacggtgat cccagatatt acatccaggt ggcggaattc
     1021 aatgcactcg agaattttcg cgtactcagg ccgggcagtg acctgacctt tccaccgctg
     1081 gaaaaggcga cgcgttgagg tgagcaacga atccaccatt cccacgccgg cgacgcctga
     1141 tgtctgcacg ttgacggtcc tggttgaggg gcaagagatc agcagtgaat ttcacgtctt
     1201 gtcgctttcg atcagccgcg aattgaaccg gattccggcc gcaacgctgc agatacagga
     1261 tggcgatgcg gccaagggga cgttcgcggt cagcgatacg gacagcttcg tgccggggaa
     1321 aggaattgaa atccagttgg ggtatcgctc gaacaacgaa acggtattca agggaatcgt
     1381 cgtcaaacag gcgatcagga tacgcaaggg tggcaatgtt ctgactgtcg aatgccggca
     1441 tgatgcggtg aggatgacca gcggggccag gagccgttat tttgtcgacc agaaggacag
     1501 cgatgtcatg gaggaagtga tcggctttta cgggttgcag aaatccgtcg agtcgaccct
     1561 ccccgacctg aaggaagtgg ttcagtatga caccagcgac tgggatttcc tgctctgtcg
     1621 ggccgaggcg aatggccagg tcgtgctggt ggaagatgac aaggtcagtg tcggcaaacc
     1681 gctgacggca gccacggccg tgctgaagat tgcttatggc gcgacagtac ttgaactgga
     1741 cgcggaaatc gacgccagat ggcaaagcaa aggcattacc gcgagtagct gg
//
LOCUS       DAORHD010001127         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86523_length_1786_cov_4.393414, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001127 DAORHD010000000
VERSION     DAORHD010001127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86523_length_1786_cov_4.393414"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..733)
                     /locus_tag="PKO06_19315"
     CDS             complement(56..733)
                     /locus_tag="PKO06_19315"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNV71863.1"
                     /translation="MELQCPISLVVFDYGNVLARVDHARFFARLTGAPVGVARELVRR
                     FYGQHSIGADYESGTLTFDQFHERLAEGIAEFSRGHSLGSVDQLRLGPWEKPAFLSAF
                     CDMFDNLEETWQFLRELKPYYRIGLLSNTNAVHFEHVISKIPVFELFDQITMSWQVGC
                     MKPDRRIFEDVVRKFGGSPTEILYLDDIARYVEAARQSGFQALVFDPERRNELLASLR
                     RQLLPRP"
     gene            808..>1786
                     /locus_tag="PKO06_19320"
     CDS             808..>1786
                     /locus_tag="PKO06_19320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HNV71864.1"
                     /translation="MNEITSGKKIFVNRTLNMNFIKLVGFDMDYTLVTYNVPAFENLV
                     HQLVVDKLIHEKGYPNALRKLRFDPQFIVRGLVLDTELGNLLKVNCFGYVKKAAHGTR
                     FMTFEEQKKAYNAPAIDLSDSRYYIVTTLFSLAEAHVFAQLVTLLEETGKPLSYRQIF
                     HDIREMVDIVHQEGILKSTVLKHPDQYLYREPKIIEALQRLKANGKKLALITNSDYEY
                     SNGAMNYCFNPYLKHPWQDLFDIIVVASSKPGFFQQSNKFLRVNRDDGHLRNFYEPLQ
                     WGGIYQGGNARTFERDLGLNPSEILYCGDHIFGDVVTLKEAIGWRTGLIL"
BASE COUNT          446 a          503 c          466 g          371 t
ORIGIN      
        1 tccctgaacc cgttcttcgc actctcgacg ggcagcgaga tcgctgcccg ttttttcatg
       61 ggcgcggcaa cagctgtcga cgcagtgacg caagcagttc attcctgcgt tccgggtcga
      121 acaccagagc ctgaaaccct gactggcggg cagcttcgac atagcgggcg atatcatcga
      181 gatacagaat ctctgtgggc gagccgccaa actttctcac cacatcttcg aagattcgtc
      241 ggtcgggctt catgcagccg acctgccacg acatggtgat ctgatcaaac agttcaaaca
      301 ccggaatctt cgagatcacg tgttcgaagt gcacggcatt cgtgtttgac agcaacccga
      361 tgcggtagta cggcttgagt tcacgcagga actgccaggt ttcctcgagg ttgtcgaaca
      421 tatcgcagaa ggccgacaga aaagcgggct tctcccaggg tccgagccgc agttgatcga
      481 cagagcccag agaatgccca cgggagaact cagcaatacc ttcggccaga cgttcgtgaa
      541 actggtcgaa ggtcagggtt ccggattcat agtcagcccc gatggagtgc tgaccataga
      601 accgcctcac cagctcgcgg gcgacgccga caggcgcgcc ggtcagtcgg gcaaagaagc
      661 gggcatgatc gacgcgggcg aggacgttgc cgtaatcgaa gacgacgagc gagatgggac
      721 actgaagttc catgacggtc tctacttgcc agaatcagcg tcttgcggta caaatgaacc
      781 cgaaccatac acaaaggaac gcaagatatg aacgagatca catcaggaaa aaagatcttt
      841 gtcaaccgca ccctcaacat gaatttcatc aagctggtcg gcttcgacat ggattacacg
      901 ttggtcacct ataacgttcc ggcattcgaa aatctcgtgc atcagctggt cgtcgacaaa
      961 ctgatccacg aaaaggggta tccgaatgcg ctgcgcaaac tgaggtttga tcctcagttc
     1021 atcgttcgcg gactggtact cgacacggaa ctcgggaatc ttctcaaggt caactgcttc
     1081 gggtatgtca agaaagcggc gcatggcacg cgtttcatga cgtttgaaga gcagaaaaag
     1141 gcctacaatg ccccagccat cgatctgagc gacagtcgtt attacatcgt cacgacgctt
     1201 ttttcactgg ccgaagccca cgtcttcgcc cagctggtca ccttgctcga agaaaccggc
     1261 aaaccgcttt cgtatcgcca gatctttcac gacatccgtg aaatggtcga catcgtgcac
     1321 caggaaggga tcctgaaaag caccgttctc aaacaccccg accagtattt gtaccgggaa
     1381 ccgaaaatca tcgaggcctt gcagcgcctg aaagccaacg gaaagaaact cgctctgatt
     1441 accaattccg attacgagta cagtaacgga gccatgaact actgcttcaa tccctatctg
     1501 aaacacccct ggcaggatct gttcgacatc atcgtcgtgg catcgagcaa gccgggcttc
     1561 tttcagcaaa gcaacaagtt cctgcgcgta aaccgcgatg acgggcatct gcgcaacttc
     1621 tacgaacctc ttcagtgggg tggcatctat cagggtggga atgcccgaac tttcgaacgc
     1681 gatctcggtc tgaacccctc cgaaattctg tactgcggcg accacatctt cggcgatgtc
     1741 gtgactttga aagaggcaat tggctggcgt accggtctga ttctgc
//
LOCUS       DAORHD010001128         1786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86532_length_1786_cov_3.794339, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001128 DAORHD010000000
VERSION     DAORHD010001128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86532_length_1786_cov_3.794339"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1786)
                     /locus_tag="PKO06_19325"
     CDS             complement(<1..>1786)
                     /locus_tag="PKO06_19325"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV71865.1"
                     /translation="TLPPSDSVFGVSYHDVLIFAGVVLVALVVPFLYSRMTTENFLTP
                     KEFVARISLAFIGSVFCLRFLLEDRLRLSRSSVDLPLALFFGFSVMSLAWNYNVPSAI
                     RDLRGTFLILLLVPLIMNSIRKQWQFDTLLWAMLFTGLATGTLGIMEAYNLYFRWDPS
                     HGWVFARDEIFNGQIDYQATYLPLFPQLASPNYAMESIVSTFGNRNYLGTFTMFTAFV
                     PLAFYFYYERTWMKVASLGLFGYTIMGLYVTRCRAALLGLIVGFAFMTLLLLIFDRQW
                     RFVKRNKNFFLAVALIGASLFLFAVTSVRTSSFMQKIQNTFTMDRSVSNTYERVWVWY
                     ATYMSFAQNPVKWLIGSGFGSYKHFFPLQEAETFDDDNKETFTPVTFRQAHNDWLQLV
                     SELGLIGMALFLFLCWRFFGAIYSILKREISDVPDGSFSGRHVVLIALGAAMVAQMMA
                     AVPDFPFHRIETALYAVLMLAMVPLLAESNFFTRPLPETLVKMPYETRVGFVVLALAT
                     GMLAIHFEMRCWQADELVRKSEALLQARQTPQMVEQAKRDLMKAISIDPLPGDPYLKM
                     ASILELEGKGQEAVEWANRAWKNINFNAR"
BASE COUNT          481 a          474 c          499 g          332 t
ORIGIN      
        1 ccgagcgttg aaattaatgt tcttccaggc acgattcgcc cactccacgg cctcctgccc
       61 cttgccttcg agttccagaa tcgaggccat tttcagatag gggtcaccag gcagcgggtc
      121 gatggagatc gctttcatca gatcgcgttt ggcctgttcg accatctgcg gcgtctgacg
      181 cgcctgcaac agggcttccg atttgcgtac cagttcatct gcctgccaac agcgcatctc
      241 gaagtgaatg gcgagcatgc cggtcgcgag cgccagaact acgaaaccga cacgcgtttc
      301 gtagggcatc ttgaccaggg tttcgggcaa cggccgggtg aaaaaatttg actcggccag
      361 cagcggcacc atcgccagca tgagaaccgc atacagcgcc gtttcgatgc gatggaacgg
      421 gaagtccgga acagctgcca tcatctgtgc caccatggcg gctccgagcg cgatcaacac
      481 gacatgacga ccgctgaagc tcccgtctgg cacatcactg atttcgcgtt tcagaatgct
      541 gtagatcgct ccgaagaagc gccagcagag aaacaggaag agagccatgc cgataagtcc
      601 gagttcagaa accagctgaa gccagtcgtt atgggcctga cgaaaggtga ccggcgtgaa
      661 ggtttccttg ttgtcgtcgt caaaggtctc ggcttcctgt aacggaaaga agtgtttgta
      721 cgagccaaag ccactgccaa taagccattt taccgggttc tgggcaaaag acatataggt
      781 tgcataccag acccagacgc gttcatacgt attgctgacc gatcgatcca tggtgaaggt
      841 attctgaatc ttctgcatga acgacgaggt gcgcaccgag gtgacggcaa acaggaacag
      901 gctggcaccg atgagcgcga cagccagaaa gaaattcttg ttacgtttca cgaatcgcca
      961 ctgtcgatcg aagatcagca gcagcagcgt catgaaggcg aagccgacga tcaatccgag
     1021 caacgcggca cgacagcggg tgacgtagag tcccatgatc gtatagccaa aaagaccaag
     1081 agaagcgact ttcatccagg tacgttcata gtagaaatag aacgccagtg gaacgaacgc
     1141 cgtgaacatg gtaaacgttc ccaggtagtt gcggttccca aacgtcgaga caatggattc
     1201 catggcatag ttcgggctcg ccagctgcgg gaacaacggc agataggtgg cctgataatc
     1261 gatctgacca ttgaagattt cgtcacgggc aaaaacccat ccgtgcgagg gatcccagcg
     1321 aaagtacaag ttgtaggctt ccatgatgcc gagcgttcca gtggccaagc cggtaaagag
     1381 catggcccag agcagcgtgt caaactgcca ctgttttctg atcgagttca tgatcaaagg
     1441 taccagcagc agaatcagga aagtgcctcg tagatcgcga atcgccgatg gcacgttgta
     1501 attccaggcc agcgacatga cggaaaagcc aaagaacaac gcaagtggca gatcgacact
     1561 cgatcggctc aggcgcaggc gatcctcgag cagaaatcgc aggcagaaca cggagccgat
     1621 gaacgccagc gaaatgcgcg cgacgaactc tttcggtgta agaaagtttt cggtggtcat
     1681 gcgcgaatag agaaagggca ccacaagcgc cacaagaacc acgccggcaa aaatcagaac
     1741 atcgtgatac gaaaccccga acacgctgtc tgaaggagga agagtt
//
LOCUS       DAORHD010001129         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86702_length_1784_cov_2.980914, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001129 DAORHD010000000
VERSION     DAORHD010001129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86702_length_1784_cov_2.980914"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PKO06_19330"
     CDS             <1..587
                     /locus_tag="PKO06_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV71866.1"
                     /translation="AQAIEAGADPLDFGIVRDDAVIARSRLLEAVDAADVVVTCGGVS
                     MGKKDPFIEAFRELQVEIQVHGISIRPGKPFFFGFLRGKPIFGLPGNQASCSVTFELF
                     VRPFLTRWLKRTDPDRLELTLPLAEPMKNHSGRDHFFRARVLSQGGRQQAAPLATQDS
                     HLLTSMLGAQLLVCHPAAEPEWKAGREVCCRLID"
     gene            603..1211
                     /locus_tag="PKO06_19335"
     CDS             603..1211
                     /locus_tag="PKO06_19335"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HNV71867.1"
                     /translation="MGENWIEQEKQVLTFHKKEAPGILVAACMVELAMEKLGEHVELK
                     GSLAGISETEWCLPDAIQVMTGCTTGNRLLKVEKQLGRYALTMYDQKTGHGIRVAVRV
                     DKIDAAKYPELAQFFHRTRDPRLTTDAKLRKESGKRIREEFAKVGRAILAWEHVVVKA
                     VGKPPIPPVAICASCGESFEQHVPDSKNCRCCSGDAYFTRKP"
     gene            1215..>1784
                     /locus_tag="PKO06_19340"
     CDS             1215..>1784
                     /locus_tag="PKO06_19340"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HNV71868.1"
                     /translation="MKLHPLLKNLPPALLMDIGTGTKDVLCFTPDDTLENNIKFVVPT
                     PASLMAEQLEHVQGDLAVSGYTMGGGYLAKVLRHHREKGFRVCMEQMPAFTVRNNLQE
                     IKDSGIEVVERIESPTHVFDEIELPLYFDLMRRFGFNPDLVKVIGVSVQDHGFHTHDE
                     SSRKNRFKYFLEHLEKSAAARSLVFTPGDL"
BASE COUNT          395 a          455 c          536 g          398 t
ORIGIN      
        1 tggctcaggc gatcgaagcc ggagcggacc cactcgactt cggcattgtt cgtgatgatg
       61 cggtcattgc gcgctctcgc ctgcttgaag ccgtcgatgc ggctgacgtc gtcgtgacct
      121 gtggtggggt ctcgatgggc aagaaagatc cgttcattga ggcctttcga gaattgcagg
      181 tcgagatcca ggtgcacgga atcagcattc gaccgggaaa gccgttcttt ttcggttttt
      241 tgcggggcaa gccgattttc ggcttaccag gaaaccaggc gagttgctcc gtgacctttg
      301 agctttttgt cagaccgttt ctgacgcgct ggctcaaacg cacggacccg gacaggctgg
      361 aactgacgct gccgctggcg gaaccaatga aaaaccactc ggggcgcgac cattttttcc
      421 gagcccgagt gctcagtcag ggcggacgtc aacaagctgc gccgctggca acacaggatt
      481 cgcatctgct cacttcgatg ctgggtgccc agttgctggt ctgccatccc gccgctgagc
      541 ccgagtggaa agccggacgc gaggtctgtt gtcgacttat cgattaacca agggggggga
      601 ctatgggcga aaactggatt gagcaggaaa aacaggtact gacttttcac aagaaagagg
      661 ctcctggcat tctcgtggcc gcgtgcatgg ttgaactcgc catggaaaag ctgggtgagc
      721 atgtcgagtt gaaggggagt ctcgccggta tcagtgaaac ggagtggtgt cttcccgatg
      781 ccattcaggt tatgaccggc tgcacaaccg ggaatcgtct gctgaaagtc gaaaaacaac
      841 tcggacgcta tgccctgacc atgtatgacc agaaaaccgg acatggcatt cgcgttgccg
      901 ttcgcgtcga caagatcgat gccgcgaagt atcccgagtt agcacagttt ttccatcgca
      961 cccgtgaccc gcgtctgacg accgatgcga aactgcgcaa agagagcgga aaacgcatca
     1021 gagaagaatt tgcgaaagtc gggagagcca ttctggcctg ggagcacgtg gtggtcaagg
     1081 cagtgggaaa acctccgatt cctccggtgg ccatctgcgc gagctgtggt gaatcttttg
     1141 aacagcatgt gcccgacagc aagaactgcc gttgctgtag tggcgatgcg tatttcacac
     1201 ggaaaccctg attcatgaag ctgcatccac tgttgaagaa tctgccacct gccctgttga
     1261 tggatatcgg gacgggaacc aaggatgttc tgtgtttcac ccctgacgac acgctggaaa
     1321 acaatatcaa gttcgtggta ccgacacccg cctcgctgat ggccgagcaa ctcgaacatg
     1381 ttcagggcga tctggcggtt tcgggataca cgatgggcgg cggatacctg gcgaaagtgc
     1441 tgcgccacca tcgcgaaaaa ggttttcgcg tatgcatgga gcagatgccg gccttcacgg
     1501 ttcggaacaa tctgcaggaa atcaaggatt cagggattga ggtcgtcgag cgcatcgagt
     1561 cgccgaccca tgtgttcgat gaaattgagt taccgctgta ttttgatctg atgcgacgat
     1621 tcggttttaa tccggatctg gtgaaggtca tcggggtttc ggtgcaggat catggttttc
     1681 acacgcatga cgagtcgtcg cgcaagaacc gattcaagta ttttctggaa catctcgaaa
     1741 agtctgccgc ggcgcgatcg ctggttttca ctccgggtga tctg
//
LOCUS       DAORHD010001130         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86709_length_1784_cov_2.640254, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001130 DAORHD010000000
VERSION     DAORHD010001130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86709_length_1784_cov_2.640254"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PKO06_19345"
     CDS             complement(<1..1320)
                     /locus_tag="PKO06_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV71869.1"
                     /translation="MNQATFEALCPRLVKQIPTGAATVIEAHAGTGKTFTIEHLVIDL
                     LLRPELSDLTLEQILVVTFTDKATVELKTRIREKLEFLVSLSSSQTIPDGEATWTIGR
                     EERQRLQQALFSFDLASIFTIHGFCSRILSDQAFDGNRLFRSELGESRERFAEAFHRT
                     LRETPRLPAFLLPLLATYLSRHSPDDLGAFLWALYASGARFPAPDLAPEVPFAAAPET
                     LLRTLCDGRQALPSPIDWSKLRRSRPQAKTIDKLNRWLETVLQGLSEAAAQGNLEPLY
                     TDDFTDAIGGLRADAGNVIPVETTYQAVQNWLYAFPDLPALLAGQLFPLVQAHLERDK
                     RSHGWYDFDDLLLNTWRALTVSDDRSGFRHQIQNRFRVALIDEFQDTDPVQWNIFRRL
                     FFESDQHHTLVLIGDPKQAIYGFRGADVETYLRARDEVRDRGGRVLHL"
     gene            complement(1317..>1784)
                     /locus_tag="PKO06_19350"
     CDS             complement(1317..>1784)
                     /locus_tag="PKO06_19350"
                     /inference="COORDINATES: protein motif:HMM:NF037683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71870.1"
                     /translation="CSSGKPKPEHFLALHLHHLFLCASKRCIEQEVAYVPLALGSGSA
                     REARWHPGLPETRAREQLQTLLTDLTAGFQPYLYPFPLVETVLAPENQALTMEQWETR
                     VRSLYEATGNARPWFADQPLRTPTEYPVLPLATAREQRQRWFAEFFRAVRRSA"
BASE COUNT          381 a          480 c          571 g          352 t
ORIGIN      
        1 gagatgcagc acgcggcccc cgcggtcgcg cacttcgtca cgtgctcgca gataggtttc
       61 gacatccgcg ccgcgaaagc cgtagatcgc ctgtttggga tcgccgatca gaaccagcgt
      121 gtggtgctgg tcgctctcga aaaagaggcg gcgaaagatg ttccattgta ccgggtctgt
      181 gtcctgaaac tcatcgatga gagccactct gaaacgattc tgaatctgat gccgaaaccc
      241 ggaacgatcg tccgacacgg tgagtgctct ccaggtattg agcagcaggt cgtcaaaatc
      301 gtaccagccg tgagaacgct tatctctttc caggtgagcc tggacgagag ggaaaagttg
      361 gccggcgagg agggcaggaa gatcgggaaa agcatacaac cagttctgca ctgcctgata
      421 cgttgtctca acaggaatga cattgcctgc gtcggcacgc agcccgccga tcgcgtcggt
      481 gaagtcgtcg gtatacaaag gttccaggtt tccctgagct gccgcttcgg agagcccctg
      541 caacacggtt tcgagccagc gattgagttt gtcgatggtt ttggcctgcg gtctcgatcg
      601 ccggagctta ctccaatcga tgggtgacgg aagcgcctga cgtccatcgc agagtgtgcg
      661 tagcaacgtc tctggtgccg cggcaaaggg aacctcagga gccaggtcgg gcgcgggaaa
      721 acgtgccccg gaggcataca aggcccagag aaaggcgccg agatcgtccg ggctgtgacg
      781 actcaaatac gtggcgagaa gcggcagaag aaaggcagga agccgcgggg tttcgcgcag
      841 cgtgcgatga aaggcttccg cgaagcgctc acggctttcg ccgagttcgg accggaacag
      901 gcgattgccg tcgaaggcct gatctgacaa tatccggctg cagaacccgt gaatggtgaa
      961 gatcgacgcg agatcaaagc tgaacaacgc ttgctgcagt cgttgacgtt cctcgcgtcc
     1021 tatcgtccag gttgcctcac catcaggaat agtttgcgag gatgaaagcg aaacaagaaa
     1081 ctcgagtttt tcgcgaatgc gggtcttgag ttcgactgtg gctttgtcgg tgaaggtgac
     1141 gacgagaatc tgctcgagcg tcaggtctga gagttccgga cgcaggagga ggtcgatgac
     1201 cagatgttcg atggtgaagg ttttgccggt tccggcatgc gcctcgataa ccgtggcggc
     1261 accggtggga atctgtttga ccagtcgcgg gcacagagct tcgaaggttg cctggttcat
     1321 gcgctcctcc ggaccgcccg aaagaactcc gcgaaccacc gctggcgctg ttctcgtgct
     1381 gtcgcgagag gcaaaaccgg atactcggtg ggtgttcgca gcggttggtc ggcaaaccac
     1441 ggtcgggcgt tgccggttgc ttcatacaac gatcgcacac gggtctccca ctgttccatt
     1501 gtgagcgcct gattctcagg tgcgaggacc gtttcgacca gcggaaacgg gtacaaatat
     1561 ggttggaacc cggctgtcag atcagtcaga agtgtctgta actgctcgcg ggcacgcgtt
     1621 tccggcagac cagggtgcca cctcgcttcg cgggcagaac cgctgcccag ggccagcggc
     1681 acataagcca cctcctgctc gatgcagcgc ttactggcgc agagaaacag atgatgcagg
     1741 tgcagggcga gaaagtgttc cggcttgggt tttccggagc tgca
//
LOCUS       DAORHD010001131         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86930_length_1781_cov_2.709154, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001131 DAORHD010000000
VERSION     DAORHD010001131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86930_length_1781_cov_2.709154"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKO06_19355"
     CDS             complement(<1..207)
                     /locus_tag="PKO06_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020465131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71871.1"
                     /translation="MSELIHAERISKSVGWWKKEIIVDQVSFTLNAGEIMGLLGPNGA
                     GKTTTMKMLLGLTSISSGTARVLGT"
     gene            complement(293..1129)
                     /locus_tag="PKO06_19360"
     CDS             complement(293..1129)
                     /locus_tag="PKO06_19360"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV71872.1"
                     /translation="MTSQPMHLVMYLSPAGSTEKVGLAIRDRLIEQARPTITWNLSEP
                     GLPKSVSDSRQPLCVWLGTPVYIDRALPIVCEALSYLPADRTRWGAGFAVYGAVTSGL
                     ALRDLQETFTRRGILPLGQMKVVAHHSQFPPDPGTPGAGRPSFDDLKTVHAYVDAVLI
                     KLDTGSASPLDLSAHDYQTPKMRQFASNKSLDILRQAGGYPPTDQSKCVRCGMCVTVC
                     PVKAIELNPFPIRLESCIFCEKCIQICPRQAYPFDHEAPRQMIRGLETMSDEEHVTRF
                     FT"
     gene            complement(1145..>1781)
                     /locus_tag="PKO06_19365"
     CDS             complement(1145..>1781)
                     /locus_tag="PKO06_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV71873.1"
                     /translation="LHQVLPRAPHAIVYGHQGIVQSRFSIRPPLTKPIEMPESSRQAL
                     HCLPNERWVQVEEPIDIDAHCHLTGPIPRLTEYEDTGGPFFLDAEGRNPDLIDDDQAL
                     WFRTAQGLVIVVGCCHSGLINTVRRVVTLSGESCIDTIIGGFHLLTADSRRLRETMTA
                     LRSFRPRLLVPCHCTGEQVMDMLLQEFSGGAEIGQTGRRYQWHDGDEPDHF"
BASE COUNT          369 a          506 c          540 g          366 t
ORIGIN      
        1 ggttccgagc acccgggcag taccggaact gatgctggtc agccctagga gcattttcat
       61 cgttgtcgtc ttgccggcgc cgttgggccc gagcaagccc atgatttctc cggcattcag
      121 cgtgaatgac acctggtcga caatgatctc tttcttccac catccgacgc tcttgctgat
      181 gcgctccgcg tgaatcagtt cgctcataag tggctgtcct ccatcacgaa gaatctcggg
      241 gtgttagacc ggttttccgg gtcctgggtt ccgtcattcg agtgttttct catcacgtga
      301 aaaacctcgt gacgtgttcc tcgtcgctca tggtctcgag cccgcgaatc atctgacgtg
      361 gggcctcgtg atcgaacggg taggcttggc gcgggcagat ctggatgcac ttttcgcaga
      421 aaatacagga ctcgagccgt atgggaaagg ggttcagttc tatggctttc accgggcaca
      481 cggtgacaca catgccgcat ctcacgcact tgctctgatc ggtgggagga tacccgcccg
      541 cctgccgcag aatatcgaga ctcttgttgc tcgcgaactg ccgcatcttc ggtgtctggt
      601 agtcgtgagc cgagagatcg aggggcgagg cagaccccgt gtcgagcttg atcaggacag
      661 cgtcgacgta ggcgtgaacc gtcttcagat catcgaacga tggccggcct gctccggggg
      721 tgccagggtc tgggggaaac tgagaatggt gtgccacgac cttcatctgc cccagcggca
      781 gaatgccgcg acgggtgaag gtttcctgca gatcgcgcag ggccagaccc gaggtgaccg
      841 cgccataaac cgcaaaccct gctccccagc gtgtacgatc ggccggaaga taggacagtg
      901 cctcgcaaac gattggcagg gctcggtcga tgtataccgg ggtgcccagc cagacacaga
      961 gcggctggcg tgagtcggaa accgacttgg gcagacctgg ttctgacagg ttccaggtga
     1021 tcgtcggtcg agcctgttcg atcagacgat cgcgtatggc caatccgacc ttttccgtgg
     1081 atcctgccgg cgaaagatac atgacgagat gcatgggctg agatgtcata atggctcctg
     1141 ttcgctagaa atgatcgggc tcgtcgccgt catgccactg ataacgtcgg cctgtctgcc
     1201 cgatttcagc cccgccagaa aactcttgta gaagcatgtc catgacctgc tcacccgtac
     1261 aatggcacgg aacgagaaga cgaggccgaa acgatcgcag ggcggtcatc gtctcgcgta
     1321 aacgacggga gtcagccgtc agcagatgaa agccgccgat aatcgtgtcg atgcacgatt
     1381 caccgctgag cgtgacaacg cgccgaacgg tattaatcag accggaatga caacacccga
     1441 cgacaatgac caggccctga gcggtgcgaa accagagcgc ttgatcatcg tcgatcaaat
     1501 cggggtttcg cccttcggcg tccagaaaga acggcccgcc ggtgtcttcg tattccgtca
     1561 gacgaggaat cggaccggtg agatggcagt gggcgtcgat gtctatcggt tcctccacct
     1621 ggacccatcg ctcgttcggc aggcagtgca atgcctgccg agacgattca ggcatctcga
     1681 tcggcttcgt cagaggcggt cgaatactga aacgagactg gacgatgccc tgatgtccgt
     1741 agacgatggc gtgtggcgct cgcgggagaa cctgatgcag a
//
LOCUS       DAORHD010001132         1780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_86982_length_1780_cov_3.513043, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001132 DAORHD010000000
VERSION     DAORHD010001132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86982_length_1780_cov_3.513043"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1780
                     /locus_tag="PKO06_19370"
     CDS             129..>1780
                     /locus_tag="PKO06_19370"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV71874.1"
                     /translation="MRSFAVRAVFITILTITLFSSTIFFVTIPALESHIMDQKRELIR
                     ELTTSAWNILAKFENDERTGIVTREEAQKQAILQIRNLHYGQQMKDYFWINDMTPRMV
                     VHPYRSDLDGKDLTTFADPDGKRLFVEVVRTVKEHGAGYVSYRWQWKDDSARVVPKLS
                     YVKGFEPWNWVIGTGVYVEDVQNEIRSAKQQLLLISLAILGIASLFLFYLLWDSLRTE
                     RKRRLAEQALKNSEEKYRLLVESAGECIVMAIGNERLFANQSMLQLLGYTPEAFAPLR
                     IDEIIGLTEDERASGHSYPVRLMRGEAVPSRYETFLRTSQGERREVTLSLSRITIQEK
                     TGFIAVATEITRRRQREIEQERLLGELNETVLFLNRSVKNICRRDLVTVKAGSTLGDV
                     CRKMVEENLPAVLVAEEAGGVRGLLSREGAFRRLADSSRAWSEPIGAAAIEQSAGIPQ
                     QAMVAEAVLALEKHEFHPLLVMAEDGSVAGVLTEREISLLNRYSPTVLLREIAAAESS
                     EALIRANRRLPELVRVLVRNGIRTTHINRIITQNSDAVLERAV"
BASE COUNT          379 a          487 c          537 g          377 t
ORIGIN      
        1 gccctcggaa acctccgcgg agcctctgcc gatcaagtga gtgggtcgac aggaacaacg
       61 aacccggggg gaaaccttcc cctcgggttg ttcttttgcc ctgccacaca acgtgatacc
      121 ctgaagctat gagatcattc gctgtacgtg ctgttttcat tacgattctg accatcacgc
      181 tcttctcgag tacgatcttc tttgtcacga ttccggctct tgaaagccat atcatggatc
      241 agaaacgcga attgattcgt gaactgacca cttctgcctg gaacatcctc gccaagttcg
      301 agaatgatga gcgtaccgga atcgtgacgc gggaggaagc tcagaagcag gccattctgc
      361 agattcgcaa cctgcactac ggccagcaga tgaaagatta cttctggatc aacgacatga
      421 cgccccgcat ggtcgtccat ccgtatcgtt cagatcttga tggcaaggat cttacgacat
      481 ttgccgaccc ggacggaaaa cgtctcttcg tcgaggtggt ccggacggtg aaagaacatg
      541 gggccggata cgtcagttat cggtggcagt ggaaggatga ctcggcgcgc gtggttccaa
      601 agctctctta cgtgaagggg ttcgaaccct ggaactgggt catcgggacc ggggtatacg
      661 tcgaagatgt gcagaatgag atcagatcag cgaagcagca actgctgctc atctcgctgg
      721 ccattctggg cattgcttcg ctcttcctgt tctacctgct ctgggacagc ctgcgcaccg
      781 agcgaaaacg acgtctggca gagcaggcgc tcaagaattc ggaagagaaa taccgtctgc
      841 tggtcgagtc tgccggcgag tgcattgtca tggctatcgg gaacgaacgc ttgtttgcca
      901 atcagagcat gctgcagctg ctgggataca cgccggaagc cttcgcgccg ttgcgtatcg
      961 acgagatcat cggcctgacc gaagacgagc gggcttcggg gcacagttac cccgtccgac
     1021 tgatgcgcgg tgaggcggtt ccgtcacgtt atgagacctt tctgcgaacc agccagggcg
     1081 aacgtcgtga ggtgaccctg tcgttgtcgc gcatcacgat tcaggagaaa actggtttca
     1141 tcgcggttgc gacggagatc acccgccggc gacagcgcga gatcgagcag gagagactgc
     1201 tcggcgagct gaatgagacc gtgttgtttc tgaatcgaag cgtcaagaac atctgccggc
     1261 gcgacctggt cacggtcaag gcgggcagca cactgggcga cgtctgccga aaaatggtcg
     1321 aagagaacct gccggcagtg ctcgtcgcgg aagaagccgg cggagttcgt gggctgcttt
     1381 cccgagaagg cgcgttccgg cgtctggccg attcgtcgcg agcctggtcc gagccgattg
     1441 gggccgctgc gatcgagcag tccgcgggaa ttccccagca ggcaatggtc gctgaggccg
     1501 tgcttgcgct cgaaaagcac gagtttcatc cgcttctggt gatggctgaa gatggatcgg
     1561 tcgcaggggt tctcaccgag cgcgaaattt cgctgctgaa ccggtactct ccaacggtgc
     1621 ttctgcgcga aattgctgca gccgagtcgt cagaagcatt gattcgagcg aatcgtcgtc
     1681 ttccggaact ggtacgcgtg ctggttcgta acggtattcg cacgacccac atcaatcgaa
     1741 ttatcacgca gaactcagat gcggtcctcg agcgtgccgt
//
LOCUS       DAORHD010001133         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87079_length_1779_cov_3.367749, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001133 DAORHD010000000
VERSION     DAORHD010001133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87079_length_1779_cov_3.367749"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1779
                     /locus_tag="PKO06_19375"
     CDS             <1..>1779
                     /locus_tag="PKO06_19375"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71875.1"
                     /translation="EFNRVVTLDAKHLSAQMSLATIYMNRDLLDKALNSLNAVVQIEP
                     DHFEAHMQLGRIYTLKQAQDRAIQVYKGAISLNPQSAQAHLRLADVYLQLEKTSDALK
                     EFSNTLNIDPNQIDALTALGEIYYNLEQFDSAANCYRRLVELEPQNHTVRYALAESYN
                     HLGEPEKALDEYLKVVEINEERIDALIRLGQLYSAKEKWQEAAKYYKKVFAYDPQNSR
                     IHFELGKVFDRLNLLAEALNEFEAALKDDQKNAQIVTWMGLILKKQGNLDAAIDRFLK
                     AIDLDQSNPMAHQELGKAYLHKGRVDKAIQEFQDALRLAPNNLELNLELAKAYMSQGI
                     LDEAIDAYKNVIRLDPENASAHFELGMINSNQGNLDDALKAFRSVIQLNPSHSRAHQE
                     LGRLLREKGQTQEAISALSRALELDPNNAVLNYELGDVFYRDGQFPNAVKVFNRALEL
                     QPDYVDVYQKLGAIHSSQEEWAKAKELYQKAIELEADNYTVYHDLGEACEKLGDTDGA
                     INALERALHFKPGNVSIYYRLGSKYKQKGDFAKMLELFTKAVELSPRNPLFHFEMGEA
                     LKNLGQTDQAMQKYEEALGLNADLLEC"
BASE COUNT          457 a          508 c          476 g          338 t
ORIGIN      
        1 gagttcaatc gcgtggtcac tcttgatgcc aagcatcttt ccgcgcagat gagtctggca
       61 accatttaca tgaaccgcga cctgctcgac aaagcgttga attcactcaa tgccgtggta
      121 cagatcgaac ccgaccactt cgaagcgcac atgcagctcg gacgcatcta cacgctgaag
      181 caggctcagg atcgcgccat tcaggtgtac aagggtgcga tctccctgaa cccgcagtcg
      241 gcgcaggctc atctgcgact ggccgatgtc tatctgcagc tcgaaaaaac ctctgacgcg
      301 ctgaaagagt tctcgaacac gctgaacatc gaccccaacc agatcgacgc tctgacggcg
      361 ctcggagaga tttactacaa cctcgaacag ttcgattccg cagcgaactg ctatcggcgc
      421 ctggtcgaac tcgagccgca gaaccacacg gtgcgctatg cgctggcaga atcttacaac
      481 catctgggcg aaccggaaaa agcactcgac gaatatctga aggtcgttga aatcaatgaa
      541 gaacgcattg acgcgctgat tcgactcgga caattgtatt cggccaagga aaagtggcag
      601 gaagcggcca agtattacaa gaaagtgttt gcctatgatc cccagaattc gcgcatccac
      661 tttgaactgg gcaaggtatt cgaccgcctg aatctgttgg ccgaggctct gaacgagttc
      721 gaagccgcac tcaaagacga tcagaagaac gctcagatcg tcacctggat gggcctgatt
      781 ctcaagaagc aagggaatct cgatgccgcg atcgatcggt tcctcaaagc aatcgatctc
      841 gatcagagca acccgatggc gcatcaggaa ctggggaagg cctacctcca caaggggcgg
      901 gtcgacaaag ccattcagga gttccaggat gcgctgcgtc tcgcgccaaa caacctcgaa
      961 ctcaatctcg aactggcaaa agcctacatg agccagggga ttctcgatga agccatcgat
     1021 gcctacaaga atgtcatcag gctcgacccg gagaatgctt ctgctcactt cgagcttggc
     1081 atgatcaatt ccaatcaagg caacctggac gatgctctca aagcatttcg cagcgtcatt
     1141 cagctgaatc cgtcgcactc ccgcgctcac caggagctgg ggcggttgct gcgggaaaag
     1201 ggacagacgc aggaagccat cagcgctctg tcacgcgccc tcgaactcga tccgaacaac
     1261 gcggttctga actatgaact gggtgatgtc ttctatcgcg atggccagtt cccgaatgcc
     1321 gtgaaggtct tcaaccgggc gctcgagttg cagcctgact acgtcgatgt ctaccagaag
     1381 ctgggggcga tacactcgag tcaggaagag tgggcgaaag ccaaggagct gtaccagaag
     1441 gccatcgaac ttgaggccga caactacacg gtctatcacg atctcggcga agcctgtgaa
     1501 aaactgggcg atacggacgg ggcgatcaac gcgctcgagc gcgccctgca cttcaagccg
     1561 ggcaacgtca gcatctacta tcgtctgggt tcgaaataca aacagaaggg cgacttcgcc
     1621 aagatgcttg agttgttcac caaggcggtc gaactttccc cacgcaaccc gctgttccac
     1681 ttcgaaatgg gcgaggctct gaagaacctc gggcagaccg atcaggccat gcagaaatac
     1741 gaagaagctc ttggcctcaa tgccgacctg ctcgagtgc
//
LOCUS       DAORHD010001134         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87166_length_1778_cov_3.349391, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001134 DAORHD010000000
VERSION     DAORHD010001134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87166_length_1778_cov_3.349391"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKO06_19380"
     CDS             complement(<1..756)
                     /locus_tag="PKO06_19380"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HNV71876.1"
                     /translation="MKRIRVVQWGLGAMGSGMVRLMLEKEGLQVVGAIDSRKEYVGKD
                     LGDVVEAGKKLNLKVVDNPKAILKKSDVDMVMMATNSFTREVFDDLHQVVEHGIDCLT
                     IAEEMAFPDAGEYDLSQSLDVLAKQKKCRVLGTGINPGFVLDALIIMMSGVCHNVTKI
                     EASRINDLSPFGPTVMRTQGVGTTPEEFKQGLAQGTIVGHIGFRESIFMISEALGLGV
                     DRVEEVREPIVSTVDRETKYIKIFLHGDVFDAED"
     gene            complement(946..>1778)
                     /locus_tag="PKO06_19385"
     CDS             complement(946..>1778)
                     /locus_tag="PKO06_19385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV71877.1"
                     /translation="QFAPDLPLIDGDPTQIRQVLMNLVINASEAIGDKSGIISVSTGV
                     MHCVREYLAESYLDDNLTEGPYVFLEIADTGCGMTREIQQRIFEPFFTTKFQGRGLGM
                     AAVLGIVRGHHGAIKIYSEAGKGTTFKVLFPAASNHLDRRMQRQMPSDWRGSGRVLFV
                     DDEESVRAVGQLMLEKLGFTVVTAENGQDALDKYRADPESFQLIILDLIMPKMGGEEA
                     FREFRRLHPAVRVLLSSGYNESEVLPLFQGKGLAGFIQKPYNLTRLSDVLKMLFPTAK
                     "
BASE COUNT          391 a          514 c          471 g          402 t
ORIGIN      
        1 atcttcagca tcaaacacat caccatgtaa aaagatcttg atgtatttgg tttcacggtc
       61 gacggtgctg acgatcggct cacgcacttc ttcgacccga tcgacgccga gcccgagggc
      121 ttcggaaatc atgaagatcg attcgcggaa cccgatgtga ccgacgatgg tcccttgagc
      181 cagaccctgt ttgaactctt cgggggtcgt gccaacgccc tgggtgcgca tgaccgtcgg
      241 gccgaacggg gacaaatcat tgatacggct ggcttcgatc ttcgtgacgt tgtggcagac
      301 accactcatc atgatgatca gcgcatcgag cacgaaacct ggattgatgc ctgttccaag
      361 cacgcggcac ttcttctgct tggcgagcac atcgagagac tggctcagat cgtattcgcc
      421 cgcatcgggg aacgccattt cttcggcgat cgtgagacag tcgatcccgt gttcgacgac
      481 ctggtgcaga tcatcgaaaa cctcacgggt aaaggaattc gtggccatca tgaccatatc
      541 cacgtcagat ttcttcagga tggctttggg gttgtcgacc accttgaggt tcagcttttt
      601 accggcttcg accacgtcac cgagatcttt gccgacgtat tctttgcggg aatcgatcgc
      661 cccaaccacc tgcaggcctt ctttttcgag catcagacgc accatgccgc tgcccatggc
      721 tccaaggccc cactgtacta cgcggatccg tttcattgcc gatgaattcc ttccgttttt
      781 atcttctctg atgaagagta tcgcttcggg aaaacgcccg aagccatcag gagtatagca
      841 caggccgcag acgtgtcaac acacgagcac cgagaaacag gggcatttga ttgtcacgaa
      901 gatctgcgac tatcaggtat tgctcagcaa ctcatacctg gcgagttact ttgccgtcgg
      961 aaagagcatc ttgagaacgt cagacagccg ggtgagatta tacggtttct gaatgaagcc
     1021 ggccagcccc ttgccttgaa agagcggcag cacttcgctc tcgttgtatc cgctcgacaa
     1081 cagcacgcgc accgccgggt gcaggcggcg gaactcacga aaggcctctt ccccgcccat
     1141 tttcggcatg atcaaatcga gaatgatgag ctgaaaggat tctgggtcgg cgcgatactt
     1201 gtcgagggcg tcctgaccat tttcggccgt gacgacggta aatccaagtt tttcgagcat
     1261 caattgtccg acggcgcgca cgctttcttc atcgtcgacg aacagcaccc ggccgctccc
     1321 gcgccagtcg ctcggcatct ggcgttgcat gcgacggtcg aggtgattcg aagctgcggg
     1381 gaacagcact ttgaaggttg ttcctttgcc ggcctcgctg taaatcttga tggccccgtg
     1441 gtggccacgc acgattccca gcaccgctgc cataccgagc ccacgtcctt gaaacttggt
     1501 ggtaaagaag ggttcgaaaa tgcgctgctg aatctctcga gtcatgccgc acccggtatc
     1561 cgcgatctcg agaaagacgt acggaccctc ggtaagattg tcatcaagat aactttcggc
     1621 cagatactcg cgcacgcaat gcatgacccc ggtactgacg ctgatgatgc cactcttgtc
     1681 gccaatcgcc tcggacgcat tgatcaccag attcataagc acctgtctga tctgcgtcgg
     1741 gtcgccatcg atcagaggca gatcaggagc aaactgga
//
LOCUS       DAORHD010001135         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87205_length_1778_cov_2.337783, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001135 DAORHD010000000
VERSION     DAORHD010001135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87205_length_1778_cov_2.337783"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1557
                     /locus_tag="PKO06_19390"
     CDS             82..1557
                     /locus_tag="PKO06_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV71878.1"
                     /translation="MSIGVFPLKYASIKDVESALRMITPGAAAAPGGSGGLPGASPAL
                     AASQSAASVETPALLGGVRILPIERINSIIAVTSKAARLEDVKRWIERLDQPTNSSET
                     QLFVYPVQNGSAQHLASVLNGIFGGDSRTTSTPNSSTGVSPGLSSVTAGQGTQQGSSF
                     GYSSSGLTNAQSTSRQSGSNAANSQTVTSVTLAQGTRLMADDLNNAILIYGSRSEYTK
                     IEETLRKLDVPPTQVLIEASIIEVTLNDDLQYGVQWLFNDTSGSGKGTGVLSTVKGGV
                     LGGPLAGFSYTLSNSAGDVRGVLNALAEKSLVKVISSPSLMVLDNHTATIAVGNQQPI
                     RSGETVSTGGFSTTSIQYKDTGVSLAVTPSVNAGNIVTMQINQGVTDVGQVDTATGQR
                     TFLQRRIDSKVAIRSGETLVLGGLIRDNTTTGKSGIPLLQDIPVVGTLFGSNNTNTTR
                     TELLVVITPRVVRSDQEIRNVSGEMRSRMRSFSTPGETQLP"
BASE COUNT          381 a          554 c          507 g          336 t
ORIGIN      
        1 ctggtgctcg tcggtaccag aaaccaggcc gaaggctggc tcgatattgt tgcgaccttc
       61 gacatcgacc tgctcaaggg aatgtcgatt ggtgtctttc cattgaagta cgcatccatc
      121 aaggacgtcg agtctgcatt gcgcatgatc accccgggcg ctgccgcagc acccggtgga
      181 tcgggaggcc ttcctggcgc gtcccctgcg ctggccgcct cacagtccgc cgcctcggtc
      241 gagactcctg ccttgctcgg cggcgtccgg atactgccga tcgaacgcat caacagcatc
      301 atcgccgtca cctccaaggc ggcgcgactc gaagacgtga agcgctggat cgagcgactg
      361 gatcagccga ccaacagctc cgagacgcag ctcttcgtgt acccggtgca gaacgggtct
      421 gcacaacatc tcgcttcggt attgaatggc atcttcgggg gcgactcccg caccacgtcc
      481 acgcccaatt catccaccgg cgtgtctcca gggctttcat ccgtcacggc tggacagggc
      541 acgcaacagg gttcgagttt cggttactcg agctcggggc tgacgaacgc gcagtccaca
      601 tcgcgtcaga gcggcagcaa cgccgcgaat tcacagactg tgacctcggt gacactcgcc
      661 caaggcacgc ggctgatggc cgacgacctg aacaatgcca tcctgatcta tggcagccgc
      721 agcgagtaca cgaagatcga ggaaacgctt cgcaagctgg atgtgccgcc cacgcaggtt
      781 ctgatcgaag ccagcatcat cgaagtcacg ctgaacgatg acctccagta cggagttcag
      841 tggttgttca acgacacgtc cggcagcggc aagggcaccg gggtgctcag cactgtcaaa
      901 ggaggtgtcc ttggcgggcc cctggctggc ttctcgtaca ccctcagcaa ctctgctgga
      961 gacgtcaggg gcgtgctgaa tgcgctcgcc gagaagtcgc tcgtgaaggt catctccagc
     1021 ccttcgttga tggttctcga caaccacacg gcgaccattg ccgtgggcaa tcagcagccc
     1081 atacggtcgg gagagaccgt ctccaccggc ggcttctcga ccacttcgat ccagtacaag
     1141 gacaccggcg tgtccctcgc tgtcacgcct tcagtcaacg caggcaacat cgtgacgatg
     1201 cagatcaacc agggcgtgac cgatgtcggc caggtagaca cggccacggg ccagcgcacg
     1261 ttccttcaga gacggatcga cagcaaggtc gcgatccgct ctggcgaaac cctggtgctg
     1321 ggtggcttga ttcgcgacaa caccaccacg ggcaagagcg ggatcccact gttgcaggac
     1381 atccctgtcg ttggaactct ttttggaagc aataacacga acaccaccag aaccgagttg
     1441 ctcgtcgtga tcacgccgcg agtggttcgc tctgatcagg aaatcaggaa cgtcagcggc
     1501 gaaatgcgct ctcgcatgag gagcttttcg acacccggag aaacgcaact cccctgagca
     1561 ctgaagcagc cgtcaacggg tgcatacgca tccacggcga aaaaccagta ccgacattcg
     1621 catctcattt tttcgcaaga cttttcgact aggactctcg tgaaacatcc acacagaacg
     1681 aaaacatcga tctatggggc ggctgtattg ctgctttcgc tgaccgcttg tggcggtatg
     1741 attaaaaagt cgcctgaaga agcggttcgc gatcgtgc
//
LOCUS       DAORHD010001136         1778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87210_length_1778_cov_2.204295, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001136 DAORHD010000000
VERSION     DAORHD010001136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87210_length_1778_cov_2.204295"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..567
                     /locus_tag="PKO06_19395"
     CDS             103..567
                     /locus_tag="PKO06_19395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71879.1"
                     /translation="MSTQLQVRDVLNRFVEVHQQRREVFRLLRTKVRTERFRVLLDGL
                     IRQQERILQGLQAQIDGMSTDLLNTWFKQPLSPPAEEWNLEQIPFTDDEFETVFVPNL
                     IQRYNENAMAFLSEARTRAQIPELTELFASLMQVTEQELLKQWEMTQAVREL"
     gene            958..>1778
                     /locus_tag="PKO06_19400"
     CDS             958..>1778
                     /locus_tag="PKO06_19400"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV71880.1"
                     /translation="MIDPIPLRLRFKAMYAELVVLATLPVYLGAVGLYGLKVLAVLAL
                     SVCTGIVFDVMARRLGDAVYTRSTWPIWFVVPLAFPPGAPLWLVPLAVGFALLFGCLL
                     FGGYGRSLFPVAAMAIVFLSLGYPAEVNILTKPFADHLAGFSTYASQVPVSNTVFSDL
                     NRFADVSPKAFFHGLMPGNIGESYAGLLIGIGLLFLLVGILDPRFVFSAVSTTVILSF
                     YGHTAYPQQVLSPVFQMLAGGFLVYLVFFVSSDPFALPRTPEGRWIGGFLFGLFT"
BASE COUNT          335 a          490 c          513 g          440 t
ORIGIN      
        1 aagggggtgt gtttgaaccc caaaaagcct tgtggtagag tgaacgacag agagttcgag
       61 agagagccgc aggcgagagc tacgtcaatg tgagggggat acatgtcaac tcagcttcaa
      121 gtgcgagacg tgctgaatcg cttcgtcgaa gtgcatcagc agcggcgcga ggtgttccgt
      181 ctgctgcgaa ccaaggtgcg caccgaacgg tttcgagtgc ttctcgacgg cctgatccgc
      241 cagcaggaaa gaattcttca agggctgcag gcgcagatcg acggcatgtc gaccgacctg
      301 ttgaacacct ggttcaaaca accgctctca ccgcccgccg aagaatggaa cctcgagcag
      361 atccccttca cggacgacga gttcgagacc gtttttgtcc cgaacctgat tcagcggtac
      421 aacgaaaacg ccatggcatt tctctccgag gcacgaacgc gggcgcagat accggaactc
      481 accgagttat tcgcttcatt gatgcaggtt accgagcaag aacttctgaa gcaatgggaa
      541 atgacccaag ctgtgcgcga actgtaacgt gtcgtgctgc catgtgataa tctggtattc
      601 aaatctcgga atctcgtaaa acaggcttgt tgacagggtt tgagaggggt ggcataattc
      661 ccgggtttct tgtcggtcag ggtacccgaa aacaggaata cagggagttc ggacagtgaa
      721 acaggtcgcg tcggtgtgca tcaattgtct cgagtgcgtt catgcatgcc cggaacgatt
      781 actgccacat tatctatatc atttcgtgca ggccggtcgt gccgaatccc tcgaagccta
      841 tggtattcag aagtgtgccg cgtgcgggtg ttgcgatgaa gtgtgcccgg cgaatctccc
      901 cttgcagtcc gtgttggttg agggcaaacg actcatgacg acttccggag agaagtcatg
      961 atcgatccga tcccgctgcg acttcgcttc aaggcgatgt acgctgagct ggtagtgctg
     1021 gcgacacttc cggtgtacct tggcgcggtc ggtctgtacg gcttgaaggt tctcgcggtc
     1081 ctggctctca gtgtctgcac cggcattgtc tttgacgtga tggcccggcg actcggcgac
     1141 gccgtataca cgcgcagcac ctggccgatc tggttcgtgg tgccactggc tttcccgcct
     1201 ggcgctccgc tttggctggt gccgctggcg gtcggattcg ccctgctgtt tgggtgtcta
     1261 ttgttcgggg gctacggtcg atcgcttttt ccggtcgcgg ccatggcgat cgtttttctc
     1321 agtctcgggt atccggccga agtgaacatt ctgacgaaac cgtttgccga tcacctggcc
     1381 ggcttctcca cttatgcgtc acaggttccg gtcagtaaca cagtgttttc agacctcaat
     1441 cgatttgccg acgtgtcgcc gaaagccttc tttcacggcc tgatgcccgg aaacatcggc
     1501 gaatcctatg ccgggctgct gatcggcatc ggtctgttgt tcctgctggt cggcatcctc
     1561 gacccacgct ttgtcttttc ggccgtatcg acaaccgtca ttctctcttt ctacggtcat
     1621 accgcgtatc cgcaacaggt actgtcgccc gtgtttcaga tgctcgcggg tgggtttctc
     1681 gtctatctgg tcttctttgt ctcatctgac ccgtttgcgt tgccgcgtac cccagaaggc
     1741 cgctggatcg gcggctttct cttcggactc tttacctg
//
LOCUS       DAORHD010001137         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87248_length_1777_cov_3.595819, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001137 DAORHD010000000
VERSION     DAORHD010001137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87248_length_1777_cov_3.595819"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PKO06_19405"
     CDS             complement(<1..574)
                     /locus_tag="PKO06_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71881.1"
                     /translation="METTCVRDEKRRESRPVAGLVRQMLLVLLLFVGTGADLWAAPAQ
                     YRFQAGKSYVFEVKIKINSEIQAYEFREAKSDERVATLTIRALSYARGIWVLDLDQDG
                     RRCRRYMRDNGQIIMSPGALPWELPFFITLPAGEPVPNKVFRSDAALPLEKGTVPARW
                     ELACRSYDQTKGRITYALAGTVTLPSDQMVK"
     gene            complement(592..>1777)
                     /locus_tag="PKO06_19410"
     CDS             complement(592..>1777)
                     /locus_tag="PKO06_19410"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNV71882.1"
                     /translation="IGKRLAPPGGGIIFRTAAEGIEERELQADFALLAKLWKRAETKS
                     RHCPPRTIVHRDMPLTLKLVREFFNDDVDRFIIDSSEEYKGIIEDCEFLSPFQRAAME
                     LYEEKTPLFEKFGVENEIRRALDFKVTLPSGAYLFIERTEALNSIDVNSGRFSGGADL
                     QETVFKVNLEAAREIARQIRLRNLSGMIIIDFIDLDDPRHRKEVVKVLKDCLKGDRNR
                     PNIYDMSELGLVQMTRRRASASLHEVLKTPCRCCAGRGRVLSPLTLANRIRTEVLGTA
                     QQFECERIRVRAHPDVVEFFNREQRTRAIELEQRAKRPLDLVPEADRVVETYTIEAVL
                     ANGSVIDRTPEDAKRPSSSERSEGDRSRPSGSRGGRSRKPLPNRTRRQSSQRRSPRKK
                     HS"
BASE COUNT          379 a          537 c          482 g          379 t
ORIGIN      
        1 gtttcaccat ctgatcggaa ggcagcgtca ccgttccagc cagcgcgtag gtgattcggc
       61 ctttcgtctg gtcataactg cgacaggcga gttcccagcg cgcaggcacc gtcccttttt
      121 cgagcggaag cgcggcgtcg ctgcgaaata ccttgttagg cacgggttct cccgccggca
      181 gagtgataaa aaagggcagc tcccacggga gtgctcctgg tgacatgatg atctgaccat
      241 tgtcacgcat gtaccggcga caacggcgtc catcctgatc cagatcgaga acccagatgc
      301 cgcgtgcgta ggaaagggcg cgtatcgtca gcgtcgccac tcgttcgtcc gacttggctt
      361 cacgaaactc atacgcttgt atctcgctgt tgattttgat cttcacttca aaaacatagg
      421 actttccagc ctgaaatcga tactgtgccg gtgcagccca aagatcggca cccgtgccga
      481 cgaacaacag cagaacgagc agcatctgtc gcacgagccc cgcgaccggt cgagattcac
      541 ggcgcttctc atcgcgcacg catgttgtct ccatctccac cttcctccag ttcaggaatg
      601 cttttttcgt ggtgaacggc gttgcgagct ctgacgacgg gtacgattcg gcaacggctt
      661 gcgcgagcgc ccgccccgac tgccggaggg tcgcgaccga tcaccctcag aacgttcaga
      721 cgaggaagga cgtttcgcgt cttcgggtgt acgatcgatg accgagccgt tcgccaacac
      781 ggcttcgatg gtgtaggttt ccacgacgcg gtccgcctcg ggtaccaaat ccagcggacg
      841 tttcgcccgc tgctccagtt cgatcgcccg cgttcgttgt tcgcgattga agaactcgac
      901 cacatcgggg tgcgcgcgca cgcgaatgcg ctcacactcg aactgttgcg cggttcccag
      961 aacttcggta cgaattcgat tcgccagagt caacggcgaa agcacgcgac cgcgcccggc
     1021 acaacaccga caaggggttt tcaatacttc atgcaagctt gcagaagctc ttcgacgcgt
     1081 catctgcacc aggcccaact cggacatatc ataaatattc ggccggttgc gatcaccctt
     1141 caggcaatct ttcagcacct tcaccacttc cttgcgatgc ctcgggtcat cgagatcgat
     1201 gaagtcgatg atgatcattc ccgacaggtt gcgcaggcga atctgacggg caatctcacg
     1261 agccgcttcg agattgacct tgaagacggt ttcctgcaga tcagccccgc cgctgaaacg
     1321 accggagtta acatcgatcg agttgagggc ttcggtgcgc tcgatgaaca gatacgctcc
     1381 cgaaggcagc gtcaccttga aatcgagtgc ccgacgaatc tcgttttcca ccccgaactt
     1441 ctcaaagagg ggcgtctttt cctcatacaa ttccatagcg gcacgctgaa atggtgacag
     1501 gaactcacag tcttcgataa tacccttgta ctcttcggat gaatcgatga tgaagcggtc
     1561 gacatcatcg ttgaaaaact cacgcaccag tttgagggtc agtggcatgt cacggtgcac
     1621 gatcgtccga ggcgggcaat gccgagactt cgtctctgcg cgcttccaga gttttgccaa
     1681 cagggcaaag tcagcctgaa gctcgcgttc ctcgatccct tcggcggcgg tacgaaaaat
     1741 gatgccgccc ccgggagggg ccagacgttt gccgatc
//
LOCUS       DAORHD010001138         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87285_length_1777_cov_2.525552, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001138 DAORHD010000000
VERSION     DAORHD010001138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87285_length_1777_cov_2.525552"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKO06_19415"
     CDS             complement(<1..261)
                     /locus_tag="PKO06_19415"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HNV71883.1"
                     /translation="MIETTSQTGGTPRIAAFDHVRAIAVLAMIVSHVTVMFGTANACM
                     TPIGRFMNEFCGTAPAAPVFMILMGIFFIYPHDKSFSQKMARG"
     gene            complement(360..1298)
                     /locus_tag="PKO06_19420"
     CDS             complement(360..1298)
                     /locus_tag="PKO06_19420"
                     /inference="COORDINATES: protein motif:HMM:NF025879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV71884.1"
                     /translation="MSVTGEKTRAEYVARVNGVVDLIQKNLDKQLSLDAVAEEAGFSP
                     FHFHRVFAGVVGEPLHAYVRRARLERAALELERMPDRSITEIALDYGFSSSANFSRAF
                     KEAFGASAREFREARTHYPELARDILKRIEDGIRRSAEQTRRQLEEAARQAGRQPPPA
                     SLRSFPATSIAYLRHYGPYEEKHKTALFRRIRSWAEQHLPGSQHRYVGICYGDRGLLS
                     ADRRRYDAGVEIPENCVVEAPAARLEIAGGQVAVFSLQTASLMVCDAWTRMHRDWLPA
                     SGFVPDDRPCVELFPDGFPQTGTAFTLEVCQAIRPR"
BASE COUNT          383 a          548 c          490 g          356 t
ORIGIN      
        1 cccgcgcgcc atcttctgcg aaaagctctt gtcatgcggg tagataaaga agatccccat
       61 gaggatcatg aacaccggag ccgccggggc cgtaccgcaa aactcgttca tgaatcgccc
      121 gatcggtgtc atacaggcat tcgcggttcc gaacatgacc gtgacatgtg agacgatcat
      181 ggccagaacg gcaatggccc ggacgtggtc aaaggcggcg atgcgcgggg ttccccccgt
      241 ctgcgacgtg gtttcgatca tggcaggtcc tctctgcgac atcggtggat tgtctctcgg
      301 aagctcggaa cgaacaccgc gcgaaactac ctcggctaca ggctcacgtc aagtcaagtt
      361 caacgcgggc gtatggcctg acagacctcg agagtgaacg ccgtgccggt ctgcggaaaa
      421 ccatcaggaa aaagctcgac gcacggtcgg tcatcgggca caaagccgct cgccggcaac
      481 cagtctcggt gcatgcgggt ccaggcatca caaaccatca gcgacgccgt ctgcaggctg
      541 aataccgcca cctgaccgcc ggcaatttcg agacgagccg ccggggcttc taccacacag
      601 ttctccggga tttcgacccc ggcatcgtac cggcgacgat cggctgatag cagcccccga
      661 tcaccataac agataccgac gtatcgatgc tgcgaccccg gaagatgctg ttccgcccat
      721 gacctgatgc gacgaaacag agccgttttg tgtttttcct cgtacgggcc gtaatgcctc
      781 agatacgcga tgcttgtcgc gggaaacgat cggagcgagg ccggcggagg ttgccgtccc
      841 gcctgacgtg cggcttcctc gagctgtcta cgggtctgtt ccgccgagcg tcgtattccg
      901 tcctcgattc gcttcagaat gtcgcgggcg agttccgggt agtgggttcg agcctcgcga
      961 aactcccgtg cactcgctcc gaaggcttct ttgaacgccc gcgagaagtt ggcagaagaa
     1021 gaaaaaccat agtcgagcgc aatttccgtg atcgaccgat ccggcatgcg ctcgagctcc
     1081 agtgccgccc gctccaggcg tgcccgacga acgtaggcat gcaggggctc gccgacaacg
     1141 ccagcaaaca cccggtgaaa atgaaacgga gaaaatccgg cttcttcggc gaccgcatca
     1201 agtgagagtt gcttgtcgag gttcttctga atcaggtcaa ccactccgtt cacacgggca
     1261 acatattccg ctctcgtctt ttcaccggtt acagacattt tcacacctca ttgatctggg
     1321 tgacttcatc ataccgccgt gcatctccag cgacaaccgc accgatgccg catgaatccg
     1381 ccagcatgaa ccgactcacc gaaagcgacg cggtcaggct gtcgcactca cccgcacgat
     1441 gcgaaatcgg gtgcgaaaac cgggcatctg ttactgatgc ccggtttcgt ttctgcgccg
     1501 gtgattccct tacgaccgac caaatcttcg tgtttctcct cagtgatcgg tagaaatagg
     1561 cggctgcatc gactccacct gacctttccc tttgcaggtc ggacacggat acggatagta
     1621 gcctggacca ccatgaagtg taccggttcc atgacagtgc gaacagggta ccagacttcc
     1681 gcgattctgc atcagtccgg ctttatccgt gtcttggtgc agaccactga agacctgttg
     1741 ctttacgagc cactgctcga aaagcaacag gtcttca
//
LOCUS       DAORHD010001139         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87402_length_1775_cov_4.476163, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001139 DAORHD010000000
VERSION     DAORHD010001139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87402_length_1775_cov_4.476163"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKO06_19425"
     CDS             <1..803
                     /locus_tag="PKO06_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV71885.1"
                     /translation="IPLELMQNGFMQRAVLGLLALAPLCALIGCLVVQFRMAFFADAV
                     SHSAFTGIALGLLIGIDPWFSLLGFGVLTGMLTMRLRRAGDLSMDTTLGVVFSTLVAL
                     GLAIISARKGLAKSLPLFLYGDILSMTDLDVLLAFALLLATLGFMAAFFNQLLMIGLH
                     EDLARVRGVPVDRLELLFAALVALVVGFSIRAVGILLVTAFLVIPAATARNLATTIRG
                     HVRWSVGLALLSALGGLALAIHFDIAAGAGMVLCAALLFACSVPLRRR"
     gene            complement(814..>1775)
                     /locus_tag="PKO06_19430"
     CDS             complement(814..>1775)
                     /locus_tag="PKO06_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71886.1"
                     /translation="LDIGEVTRLAPEWDRTLRELLHGTTLTVDDAIRSLGRSFHIGVV
                     DGEMRFDFDHSVMGRELPGFRRNPFESLVFGFCLSALNLPIYAAIEVRDEETVLRFVR
                     EWLTFHTDENLRKQDLWNRSFFDFKTYETQAGEGRPTIHGLDLLLFILRYRFFFAVHQ
                     GHLLIATQRELLNELLDANLPRFRTRANIEMRLRYEAFDRVALTTKLNWQAQTRQACQ
                     NNLGLLYQLARYRRLPPGQWSSESLRVNGFIPFCPEGGTYQDEIERNCISCSIHGDHI
                     APRQPWKANANQPLNRFLDSLKELGGSLSFTPEGIMTRVFIRR"
BASE COUNT          285 a          517 c          569 g          404 t
ORIGIN      
        1 tgattcctct cgagctcatg cagaatggct tcatgcagcg ggccgtgctg gggctgctcg
       61 cgttggcccc attgtgcgct ctgatcggtt gtctggttgt ccagtttcgc atggccttct
      121 ttgccgatgc cgtctcgcat tccgcgttta ccggcatcgc gctgggtctg ttgatcggca
      181 tcgacccgtg gttcagtctg ctgggtttcg gcgtgctgac cggcatgttg accatgcggc
      241 tgcgccgggc cggtgacctg tcgatggata ccacgctcgg cgtcgtgttt tccaccctgg
      301 tggccctcgg actggcgatc atcagtgcgc gtaaagggct ggccaagagt cttcccctgt
      361 tcctctacgg ggacattctc tcgatgaccg acctcgacgt gctgcttgcc tttgcgttgt
      421 tgctggcgac gctgggtttc atggctgcct tcttcaacca gctgctgatg attggtctgc
      481 atgaagatct cgccagggtg cgcggcgttc cggtcgatcg gctggaactg ctgtttgccg
      541 ctctggtggc cctggtcgtc ggcttctcga ttcgcgcggt gggaattctg ctggtcacgg
      601 cctttctcgt gattccggcg gcgacggccc gcaatctggc gacgacgatt cgcggccatg
      661 tgcgctggtc ggtcgggctg gcgctgcttt cggctctcgg cggactggcc ctggcgatcc
      721 acttcgatat tgccgcgggc gcgggaatgg tcctgtgcgc cgcgctcctg tttgcctgct
      781 ccgtgccgtt gcgtcgccgc tgaccaggtt ctgtcagcgt ctgatgaaga cgcgggtcat
      841 gatgccttcc ggcgtgaagc tgagcgagcc gccgagctct ttcaggctgt cgagaaaccg
      901 gttgagcggt tggttcgcat tggccttcca gggttgtcgg ggcgcaatat gatcgccgtg
      961 aatgctgcag ctgatgcagt tgcgctcgat ctcatcctgg tacgttccgc cttcgggaca
     1021 gaaggggatg aagccgttga ctcggagtga ctcggagctc cactggccgg ggggaaggcg
     1081 acgataccgg gccaactggt acaagaggcc aaggttgttc tgacaggcct gacgggtctg
     1141 tgcctgccag ttcaacttcg tggtcagagc cacacgatcg aaggcctcgt atcgcaggcg
     1201 catctcgata ttcgcgcgcg tgcgaaaacg cggaaggttc gcgtcgagaa gctcgttcaa
     1261 cagttcgcgc tgtgtggcga tcaacaggtg tccctgatga accgcgaaga agaagcgata
     1321 gcgcagaatg aacaacagca gatcgagacc gtggatcgtc gggcgccctt cacccgcctg
     1381 ggtttcatac gtcttgaagt cgaagaagga ccggttccag agatcctgct tgcgcaggtt
     1441 ttcatcggtg tgaaaagtca gccactcccg gacaaatcgc agaacggtct cttcgtcccg
     1501 cacctcgatt gccgcgtaga ttggcagatt cagagccgac aggcagaacc cgaacaccag
     1561 tgattcgaag ggattgcgcc ggaaccccgg gagctcccga cccatgacgg agtggtcgaa
     1621 gtcgaagcgc atctcgccgt caaccacacc gatgtgaaag ctgcgcccga gactgcgaat
     1681 ggcatcatca acggtcaggg tcgtgccgtg cagcagctcg cgcagtgtgc gatcccactc
     1741 tggtgcgagt cgcgtgacct ctccgatgtc gagtt
//
LOCUS       DAORHD010001140         1775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87411_length_1775_cov_3.355233, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001140 DAORHD010000000
VERSION     DAORHD010001140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87411_length_1775_cov_3.355233"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1775
                     /locus_tag="PKO06_19435"
     CDS             <1..>1775
                     /locus_tag="PKO06_19435"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV71887.1"
                     /translation="GKPFYRLTPAELAQYHMDFVTRCGVQIVGGCCGTTPEHLKAVAD
                     TVRSLTPGTRHLESLPSVASLYQSVNLTQEPAPCMVGERTNANGSKAFRDLLLNNDWD
                     GMTAMGREQGQGGAHLLDLSVAFVGRQEAQDMQKLVPLFAQQVKLPLVIDSTEPPVIA
                     AALERHPGRCIINSVNFEDGGKRLREVAAVAREFGSALICLTIDEEGMAKTADRKLAI
                     AHRLFDTLTGEFGFRPHDLVFDPLTFTVGSGEASLRDAAVQTLEAIRRIKTELPGCFT
                     ILGVSNVSFGLAPEAREVLNSVFLHEAVQAGLSLAIVNPRGIIPHFKIPEEIRHLALA
                     LLTQPDPAGASLAAFMAAFSGGIRKPEQATATTTPAERLRLKILDGDRSGLEAILDQL
                     RSTIAPIDIINLHLIPAMKEVGELFGRGEMQLPFVLQSAEVMKTAVTLLERHMEKRPP
                     DPRKKIVLATVRGDVHDIGKNLVDILLSNNGFAVTNLGIKVDIDTMIKAAQEEQAPVI
                     GMSGLLVKSTVIMKENLEELNRRQFTPTVLLGGAALTRSYVEETLQSMYHGRVLYARD
                     AMDGLAIMQKLSESEPDSLTTRTIA"
BASE COUNT          370 a          557 c          500 g          348 t
ORIGIN      
        1 ggcaagccgt tctaccgtct cacacctgcc gaactcgcgc agtatcatat ggacttcgtc
       61 actcgctgcg gcgttcagat cgtgggcggc tgctgcggca ccacccccga gcacctgaag
      121 gccgtcgccg acaccgttcg ctcgctaacc cccggaacac ggcatttgga gtctcttccc
      181 tcagtcgcca gcctgtacca atctgtcaac ctcactcagg aacccgcccc ctgcatggtt
      241 ggggaacgaa ccaatgcgaa tggttcaaag gcctttcgcg acctgctgct gaacaacgac
      301 tgggacggca tgacggcgat gggtcgcgag caagggcagg gcggcgctca tcttctcgat
      361 ctttccgtcg cctttgtggg gaggcaagag gctcaggaca tgcagaagct ggtaccgttg
      421 tttgcccagc aggtgaagct gccgctcgtc atcgattcga cggaaccgcc cgtaatcgcc
      481 gcagctctcg agcgccaccc cgggcgctgc atcatcaact cggtaaactt cgaggatggc
      541 ggcaagcgtc tgcgcgaggt agcggccgtg gctcgcgaat tcggcagcgc cctgatctgc
      601 ctgaccatcg atgaagaggg aatggcgaaa accgccgacc gcaaactcgc gatcgcgcac
      661 cgactttttg acaccctgac aggcgagttc gggttccgcc cccatgacct tgtgtttgac
      721 ccgctgacct tcaccgtcgg cagtggcgaa gcctcgctgc gtgatgccgc ggttcagacg
      781 ctcgaggcta tcaggcgcat caagactgaa ctccccgggt gcttcaccat tctgggtgtc
      841 agtaatgtat cgttcggatt ggccccggaa gcacgtgaag tgctcaacag cgtgtttctg
      901 cacgaagcgg ttcaggctgg cctgtcgctg gcaatcgtga atccgcgcgg catcattccc
      961 catttcaaga tacccgaaga gatccgccat ctggctctgg cgctgctcac ccagcccgat
     1021 ccggcggggg cttctctggc cgccttcatg gcggcatttt cgggtggtat tcgtaaacct
     1081 gaacaggcga cagccaccac gacccctgcc gagcgtctgc gactgaaaat tctcgacggc
     1141 gatcgctcag ggctcgaagc cattctcgat caactgcgct caaccatcgc tcccatcgac
     1201 atcatcaact tgcatctgat ccccgcgatg aaagaggtgg gcgaactgtt cggccgcggc
     1261 gaaatgcagc tgccgttcgt cctgcagtct gccgaggtca tgaaaaccgc tgtgactctt
     1321 ctcgagagac acatggaaaa acgccccccg gatccgcgaa aaaagattgt ccttgccacc
     1381 gttcgcggag atgtgcatga catcggaaaa aatctggtgg acattctgtt gtcgaacaac
     1441 ggtttcgccg tcaccaacct gggaatcaag gtcgatatcg acaccatgat caaggccgcc
     1501 caggaggaac aggcccctgt catcggcatg agtggcctac tcgtcaagtc cacggtcatc
     1561 atgaaggaaa acctggaaga gctgaaccgg cggcagttca cgccgaccgt actgctgggt
     1621 ggtgccgcac tgactcgcag ctacgttgaa gaaacgcttc agtcgatgta tcacggacgc
     1681 gtgctctatg cccgcgacgc catggacggc ttggcaatta tgcagaagct gtctgaaagc
     1741 gagccagatt cactcaccac caggacaatc gccag
//
LOCUS       DAORHD010001141         1771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87746_length_1771_cov_3.515152, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001141 DAORHD010000000
VERSION     DAORHD010001141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87746_length_1771_cov_3.515152"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..933
                     /locus_tag="PKO06_19440"
     CDS             160..933
                     /locus_tag="PKO06_19440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNV71888.1"
                     /translation="MKLGYPTHPRRSLLTEIDWIGRNGFDFVDLFLEPETGALGAFDA
                     VAVREALRSWNLPAVGHLAWYLPIGSPVKELRRAAIEVAASYFPALATIGISRVTIHA
                     HWASSSLFSVEESLHWQGETLAEMIRLAAPHGLQIMYEPVGMAAESTENLDKLLQCLP
                     DLGFHLDIGHFHLNQRQPADFIRHFASRLVHVHLHDNDGSRDQHLPPGIGNIDWDATL
                     TALKECYDGTVTLEVFTPEKDYVLLARDWVRRRWAGIVL"
     gene            944..1582
                     /locus_tag="PKO06_19445"
     CDS             944..1582
                     /locus_tag="PKO06_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71889.1"
                     /translation="MARRPAENLATGAAGFAGLPLAEQIASLQRELKTGREDELLQQC
                     AALTDTDPLAWLCRALAERGYLEDTRARDQVAAVLIDTPDTGVRKVLRALTVEGTPLD
                     PPRLAEVGLLLRRLLERLPFSRLTAMCLIDRTFKQMFAEQRGEYLEMVWSEIRKLLRS
                     SRVDSILLDRLVQIPAVRLEQLRQVARETGAAVDLAFAGLAFYIKKKQRHSL"
     gene            complement(1574..>1771)
                     /locus_tag="PKO06_19450"
     CDS             complement(1574..>1771)
                     /locus_tag="PKO06_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71890.1"
                     /translation="PGIVAALPAAGRPFDQLAAVAQAPPDGPIQVVCANIFCEKIIKA
                     AASARPVKSLEFRMEIFPFLK"
BASE COUNT          341 a          504 c          524 g          402 t
ORIGIN      
        1 cggaccgcga actctcgatc agactgtgag gtgcgttcgg tgaatccctt gatgtcagta
       61 aagacgatcg ataagaccct tgaagccatg ttctgcctcc tccgtttgaa taccacggga
      121 agagtatcat gttttcccgg tttcgagtag aatagcgcca tgaaacttgg atacccgact
      181 cacccacgtc gttcgcttct caccgagatc gattggatcg gccgcaacgg tttcgatttt
      241 gtcgatctgt ttctcgagcc tgaaaccggc gctctggggg cctttgatgc cgtggcggtg
      301 cgcgaggctc tgcggagctg gaacctgccg gcggtcggtc acctggcgtg gtatctgccg
      361 ataggttctc ccgtgaagga actgcgtcgc gcggcgatcg aggtggcggc ttcgtatttt
      421 ccagctctgg ccactatcgg aatttcgcga gtgacgatcc atgcccattg ggcctcgagt
      481 tcgctgtttt cggtcgagga atccctccat tggcagggcg agaccctcgc cgagatgatc
      541 agactcgctg cgcctcatgg acttcagatc atgtatgaac cggtcggcat ggccgctgaa
      601 tcgaccgaga acctggacaa actcctgcag tgcctgccgg atctcggctt tcatctcgat
      661 atcggtcact ttcatctgaa tcagcggcaa ccggcggact tcattcggca cttcgcgtcg
      721 cgtctggtgc atgtgcacct gcatgacaat gatggctcgc gagatcagca tctgccgccg
      781 ggaatcggta acatcgactg ggacgcaacg ctgacggccc tgaaagagtg ctatgacgga
      841 accgtgacgc ttgaagtgtt caccccggag aaggactacg ttctgctggc tcgtgactgg
      901 gttcgccgcc gctgggcggg cattgtgctg tagcgtgtga gtcatggcgc gacgtcccgc
      961 tgagaacctc gctaccggtg ctgccgggtt tgccggtctg cctcttgccg agcagatcgc
     1021 gagtctgcaa cgtgagctca agaccggccg cgaagacgag cttctgcagc aatgtgccgc
     1081 gctgaccgac acggacccgc tcgcctggtt gtgtcgagca ctggccgagc gagggtatct
     1141 cgaagacact cgtgcccgag accaggtggc cgccgttctg atcgacacgc ccgacacggg
     1201 agtgcgaaag gtactgcgtg cgttgacggt tgagggaacg cccctcgatc cgccgcgtct
     1261 ggcggaagtc ggactcttgt tgcgccgttt gctcgaacgt ctgcctttct cgcgtctgac
     1321 cgccatgtgt ctgatcgatc gcaccttcaa acagatgttc gcggaacagc gtggtgagta
     1381 tctcgagatg gtgtggtcag agatacgaaa actcctgcga tcttcacggg tcgacagcat
     1441 tcttcttgat cgactggtac aaataccagc agtcaggctg gagcagttgc gacaggtcgc
     1501 ccgtgaaacc ggggccgcgg ttgatctggc ttttgccgga ctagcttttt atattaaaaa
     1561 aaaacaaagg cattcacttt aaaaaaggaa aaatttccat gcgaaactcc agacttttta
     1621 ccggcctcgc gctcgcggcg gccttgatga ttttttcaca aaaaatattt gcgcagacca
     1681 cttggatcgg cccatcgggc ggtgcttggg ctacggcggc caactggtcg aacggccttc
     1741 ccgccgctgg caacgcggct acgattcccg g
//
LOCUS       DAORHD010001142         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87851_length_1770_cov_2.881050, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001142 DAORHD010000000
VERSION     DAORHD010001142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87851_length_1770_cov_2.881050"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            256..1032
                     /locus_tag="PKO06_19455"
     CDS             256..1032
                     /locus_tag="PKO06_19455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71891.1"
                     /translation="MTNLSARVTLIRKMVFSLVAAVFAVVLAGCGGGGGGGGGIVTPP
                     VDSVHTTFEVNPARIRSLGAAYNVSVIVRETRGDAIDISRMRTYRTWETNLGQTGDYY
                     AGTPRYVEGWMFTTPSGSNNIRDAVVYDTSSAYSRDLHRLQAYESRTISTQVMVGNPY
                     QSRPSGFYGIFITNSDYRTMLQSHRGMKFWVRLEGYDQRLGEEYNIMMPLEIWFDYNP
                     TDPYGGGSGSDTDPYDDPYNPYGGSTGGSSTDPYANNVKN"
     gene            1046..>1770
                     /locus_tag="PKO06_19460"
     CDS             1046..>1770
                     /locus_tag="PKO06_19460"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71892.1"
                     /translation="MNLRLVCLVCVLCLVSPVLFAAVDPFADRSNAIEPLPWRVDSWP
                     GQPSHPGNGTYPGHGTHPGHGGGSPQPTYPNTPPGYSYQSPEQMLSWAMQAYRQGDYR
                     TAADRYRQVVDRYPYDSRAGEAAFMCGESLRAAQQWYDAIQMYRRCTSSYSSYQRVDE
                     AAYYTGFCLVKVSDSRGAINEFRDFVNRYRYSALVDNAWYVLGRTHEVLGERENAIQA
                     YRTVVQSFPNSDLITEARTRLQAL"
BASE COUNT          349 a          523 c          503 g          395 t
ORIGIN      
        1 gaagagtaag gcacaaggcg gtggttctca tgtgccgaga ataacacagg cctccagacc
       61 ggtcaatccg accgggcagc accccaggaa actcgaccgg agcgagtggc gcatttccgg
      121 cacttgatgc cagatttcta tcttgacacc tgccggattt cgtgaatgtc gcttcaaatc
      181 aggttctacg cactggcatg ctgattgcta acttctcctt ccggttcacg ttcagatttt
      241 tgggagtagc gagtaatgac aaacttgtct gcacgcgtca cgttgattcg gaaaatggtg
      301 ttttctctcg tcgcggccgt ttttgccgtg gttctggccg gctgtggcgg cggaggtggg
      361 ggcggtggcg gcatcgtgac gccgcccgtc gattcggtgc ataccacgtt cgaagtgaac
      421 ccggcgcgca ttcgatcact cggagcggcc tacaacgtgt cggtcatcgt gcgtgaaacc
      481 cgcggcgatg ccattgacat ttcccgcatg cgtacctatc gcacctggga aaccaatctg
      541 ggacagacag gcgactacta tgccggaacg ccgcgctatg tcgaaggatg gatgtttacc
      601 accccaagcg gtagtaacaa catccgcgac gcggtggtct atgacaccag ttccgcttac
      661 tcccgcgatc tccatcgcct gcaggcatat gagtcgcgca ccatctcgac ccaggttatg
      721 gtcggcaacc cctatcagag ccgtccttcc ggcttctacg gcattttcat caccaactcc
      781 gactatcgga cgatgctgca gagtcatcgc ggaatgaagt tctgggtgcg tctcgaaggc
      841 tacgatcagc gtctcggcga ggaatacaac atcatgatgc cgcttgagat ctggttcgac
      901 tacaatccga ccgatcctta tggcggtgga agtggttccg acaccgatcc ctatgatgat
      961 ccgtacaacc cctatggtgg ctcgaccggc ggtagttcaa cagatccgta tgccaacaac
     1021 gtcaagaact gagaggaaca accacatgaa cctgcgtctt gtgtgccttg tctgcgtgct
     1081 gtgcctcgtg tcgccggtcc tgttcgcggc tgtcgatccg tttgccgatc gatcgaatgc
     1141 gatcgaaccg ctcccctggc gtgttgactc ctggcccgga caaccgtcgc atcccggaaa
     1201 cggaacctat cctggccacg gaacccatcc cggtcacggt ggtggatccc cgcagccaac
     1261 ctatccgaac accccgccgg ggtattccta ccagtcgccc gagcagatgc tttcgtgggc
     1321 catgcaggct taccgccagg gagactaccg cacggcggct gaccggtacc ggcaggtggt
     1381 tgaccgctat ccgtatgaca gtcgcgccgg tgaagcggct ttcatgtgtg gggaaagcct
     1441 gcgggcggcg cagcagtggt atgacgccat tcagatgtat cgccgttgta cgagttcgta
     1501 ttcttcgtat cagcgtgtgg atgaggccgc ctattatacg ggattctgtc tcgtgaaagt
     1561 gagcgactct cgtggcgcga tcaatgagtt tcgtgacttc gtgaatcgct atcgctacag
     1621 cgctttggtc gacaacgcct ggtacgttct cggtcgcacc catgaggttc tgggtgagcg
     1681 cgagaacgcg atacaggcgt accgcaccgt cgtgcaatcc ttccccaact ccgatctgat
     1741 cacggaagct cgcactcgtc tgcaggcgct
//
LOCUS       DAORHD010001143         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87905_length_1769_cov_3.837806, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001143 DAORHD010000000
VERSION     DAORHD010001143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87905_length_1769_cov_3.837806"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PKO06_19465"
     CDS             <1..1288
                     /locus_tag="PKO06_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71893.1"
                     /translation="PVQSPTGLHWGSTVAAVVSVVATDPVPVTPPPPDGKSPPSVESK
                     DDVGKTRVASASDTDATRTVSSLVAPYAWVCQSEGTARVLAWLWPGGLWLLLLAIRRW
                     TYPAEPVYWAHECIVVLSFTYAACVSVWLHGAFGTLRADLRAADTRVRDLLEQQYLEM
                     QGWGQLIVMPLVVSGIAILIRYVCSSDIFEPFTDRNLDYLFSAWLFTGFCAFMHALVP
                     VARFSRIVRVLGDAASDGRLSRSEIRRVAAFYLQVSMLATLLFILGGMVIFDMHLVYT
                     YHGNPWPGMYVFSGIQARTLSAYLAAVSSHDSFIEIVVVLLLYGGISLGSLIYFLVPQ
                     WNVHELLAHRKQKAFERAEKSLENAEKRMMSEPGEETMERYARQTMVVQAVENMPEWP
                     FDGRGFIGFLAVILLPALLVLFKEIILETIVSALTK"
     gene            complement(1305..>1769)
                     /locus_tag="PKO06_19470"
     CDS             complement(1305..>1769)
                     /locus_tag="PKO06_19470"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV71894.1"
                     /translation="MFEQSGCLDKLVGDCVIGLFGPPFFAGEPSLLALNALKSAVAIR
                     DFTTRFFQLPENADIQQSPFFKDFGVAIGVNFCSADVGMIGPNKDLTAFSSGMNNTAR
                     LQGLAHAGEILVTPSVKESADRHEPGKWQFSGPHAAKVKNVAEPLAYFKLES"
BASE COUNT          314 a          497 c          546 g          412 t
ORIGIN      
        1 accggtgcag agccccacgg gcttgcactg ggggtcgacg gtggcggcag tggtgtcggt
       61 tgttgccacc gatcctgtgc cagtcactcc accaccaccg gacgggaagt cacctccttc
      121 tgtggagtca aaagacgatg tcgggaaaac ccgagttgcg tcagcatctg atactgacgc
      181 aactcggact gtttcgagtc tggtagcgcc ctatgcgtgg gtttgtcaat cggaggggac
      241 ggcccgagtg cttgcctggc tctggcctgg cggtctgtgg ttgctgctgc tggcgattcg
      301 ccgttggact taccccgcgg aacccgtcta ttgggcgcac gagtgtattg tcgtgctttc
      361 gtttacctat gcggcgtgtg tctctgtctg gctgcacgga gcctttggca cactgcgagc
      421 cgatctgcgt gccgccgaca cccgggtacg tgacctgctc gaacagcagt atctcgaaat
      481 gcagggctgg gggcagctca tcgtcatgcc gctggtcgtc agcggcatcg ccatcttgat
      541 tcgctatgtc tgcagcagcg atatcttcga gccttttacc gatcgtaacc tggactatct
      601 gttttcggcc tggttgttta ccggcttctg tgccttcatg catgccctgg ttcccgtagc
      661 tcgtttcagc cgaatcgtac gcgttctggg tgatgcggcg agcgatggcc gtctcagtcg
      721 ttcggagatc cgccgtgttg ccgccttcta tctgcaggtg tcgatgctgg ccacgctgct
      781 gttcattctg ggcggcatgg tcatcttcga catgcatctg gtgtacacgt atcatggcaa
      841 cccctggccg ggaatgtacg tgttttcagg cattcaagcc cgcactcttt ccgcgtatct
      901 cgcggctgtg tcgagtcacg attcgttcat tgaaatagtg gtcgtgctgc tgctctacgg
      961 cggtatcagt ctcggttcgc tgatctactt cctggtaccg cagtggaacg tgcacgagtt
     1021 gctggcgcac cgcaaacaga aggcattcga acgggccgag aagagtctgg agaatgcgga
     1081 aaagcgcatg atgagcgagc ccggcgaaga gaccatggaa cgctatgcgc ggcagacgat
     1141 ggtggtgcag gcggtcgaaa acatgcccga gtggccgttt gacgggcgcg gcttcatcgg
     1201 ctttctcgct gtgattctgc tgccggcgct gcttgtcctg ttcaaggaaa tcatccttga
     1261 gacgatcgtc agcgccttaa ccaagtgacc acgcggatcg cggttcagga ttcgagtttg
     1321 aagtaggcca gcggctcggc gacattcttc actttcgcgg cgtgagggcc gctgaactgc
     1381 cacttcccag gctcatgtcg atcggcgctt tccttgaccg acggggtgac cagaatctcg
     1441 ccggcatgtg caagcccctg cagacgagcg gtgttgttca tgccggaact gaacgcggtc
     1501 agatctttgt tcggaccgat catgcccacg tcggctgaac agaagttcac accgatggcg
     1561 acgccgaaat ccttgaagaa gggcgactgc tggatatcgg cgttctctgg gagctgaaag
     1621 aatcgcgtcg tgaaatcacg aatggcgacc gcgctcttca gtgcgttcag ggcgagcaga
     1681 gatggttctc cggcgaagaa gggggggccg aacaagccga tcacacagtc gccaaccagt
     1741 ttgtccaggc agccgctctg ctcgaacat
//
LOCUS       DAORHD010001144         1769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87949_length_1769_cov_2.171529, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001144 DAORHD010000000
VERSION     DAORHD010001144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87949_length_1769_cov_2.171529"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..161)
                     /locus_tag="PKO06_19475"
     tRNA            complement(77..161)
                     /locus_tag="PKO06_19475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(125..127),aa:Leu,seq:cag)
     gene            complement(321..812)
                     /locus_tag="PKO06_19480"
     CDS             complement(321..812)
                     /locus_tag="PKO06_19480"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV71895.1"
                     /translation="MGVTGINEAISPDIQMKMKLMGTITFFEGFTSNDLLYTAQIADW
                     LKMGPGDVIFAEGDLGSAFWIVLKGTVRVVKRMPDIPDQVLAFIPTGECFGEMSIISG
                     QPRTAHCIASEEVFLFRIDGQKLSRTTDALQLKFYKRFAVVLAARLARTSQNLMNRPP
                     AAV"
     gene            1198..>1769
                     /locus_tag="PKO06_19485"
     CDS             1198..>1769
                     /locus_tag="PKO06_19485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71896.1"
                     /translation="MNRTTLAMLACCLVSAIILAGCGGGGGGGGGGEAQPPAQATYNY
                     LRAVSIPADIAETGAVAATLSRSELNVNAKISRNSIRMSIAAATQVRKSNDNVYTITS
                     TNYPTDQDYDPTDGIGEQTVIVKYISKTNVNQTKPDANTARIELEASGNTDYEVLKYE
                     NATAKYILSNPATTGDWLSVANYLLKLTETA"
BASE COUNT          415 a          479 c          462 g          413 t
ORIGIN      
        1 agattcagga tcttatgcct actctggctt gcgggttcaa gtcccgccct ggtaacgcgt
       61 gtctccggtg tgtcggtgcc cagggcggga cttgaacccg caagccagta gtaggcataa
      121 gatcctgaat cttacgtgtt tgccaatttc accacccggg cagggttctt tgagtttacc
      181 agaaccgtgt accgaaatca agtgatttgt tgtcgaaaca cccgcggggc atctctcggt
      241 tcaggcacca gcgcgacccg cgacatgccc tggccggaac cccgaaggcg gtcacgattc
      301 gtcgatcgat cgaaacatcg ctagacggcc gcgggcgggc ggttcatcag attctggctg
      361 gtacgcgcga gacgcgccgc gagcacgacc gcgaaccgct tgtagaactt gagctgcagc
      421 gcgtcggtgg tacgactcag tttctgcccg tcgattctga agaggaacac ctcttcactc
      481 gcgatgcagt gcgcggttcg aggctgtccg gagatgatgg acatctcgcc gaaacattcg
      541 ccggtcggaa tgaatgccaa gacctgatcg ggaatatccg gcattcgctt cacgactcga
      601 accgtccctt tcaggacgat ccagaaagcg cttccgaggt cgccctccgc gaagatgacg
      661 tcgcccggcc ccattttcag ccagtcggcg atctgcgcgg tatagagcag atcgttcgag
      721 gtgaatccct caaagaacgt gatcgtcccc atcagcttca ttttcatctg aatatcgggg
      781 gaaatcgctt cgttgatccc ggtcacgccc acgctttttt tggctcctgg tctgtctctt
      841 caggcctgag tcatgaaaaa acggctctcg gaatcaccca agagccgctt tcggtgccgg
      901 aggtcggagt cgaattgagg gctgtcgctc cgcgacaacc cagcatgcct caattgagct
      961 tttgatcgac ctgcggtcgc tttgagtttc ccagctttca tacaaaatca accttcacca
     1021 gttcggatag tcttcttgtt tgtaccctga cactgtgaag agataccatt taaatccgct
     1081 ccaggcaaag gtttgggtag tcgtgcagaa tcaagcactt ttttctttgt cccccacctt
     1141 atcgggggta aaatgtgaac cacttggact ctattttcga aggagaggaa tctatctatg
     1201 aatcgtacga ccttggcgat gttggcctgt tgtttggtgt ccgcaatcat cctcgctggt
     1261 tgcggtggcg gcggtggtgg cggtggcggt ggcgaggcgc aacctccagc tcaggcgaca
     1321 tacaactact tgagagcagt gtcaatccca gctgacattg cagagacagg tgcggttgcg
     1381 gcaacgctat caagatccga actcaatgtg aatgccaaga tttccagaaa cagtatccgc
     1441 atgagtattg cggctgcgac acaagtaaga aagagtaatg acaacgttta cacaattact
     1501 tccaccaact atcccaccga ccaagattac gatccgaccg atggtattgg tgaacagaca
     1561 gtaattgtga agtatattag caagaccaac gtaaatcaga caaagccaga tgcaaatact
     1621 gctcgtattg agctcgaagc ttcaggtaat accgattatg aggtgctcaa gtatgagaat
     1681 gccactgcaa agtacattct ctcgaaccca gccacaactg gggattggct ctcagtagcg
     1741 aactaccttc tcaaactcac tgaaacagc
//
LOCUS       DAORHD010001145         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87996_length_1768_cov_3.268535, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001145 DAORHD010000000
VERSION     DAORHD010001145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87996_length_1768_cov_3.268535"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="PKO06_19490"
     CDS             <1..1444
                     /locus_tag="PKO06_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HNV71897.1"
                     /translation="ALFEKVRDVCARFLNVPPESITPQKRLVEFLQLDSLGLVALLAH
                     FQEVFRVSLRDVDLATLRDFSAVCHLIMRKGNWEELCDLKTAGLTSDAMPPLLDYSED
                     GIKRRRKFLKNRHGIDPSVFGQVANADDLNGNIEGFVGYSMVPIGIAGPLKVNGEYAR
                     GDFFLPLATTEGALVSSIARGTQIITMSGGARTRVLADSLVRAPVFLFSTLDELASFK
                     TWIDANLDLIREAAEKTTRHGKLLDIENYPMGTTMCLRFVYSTADASGQNMTTIATHA
                     AMDYIKKAFPGKMVDCFLEANMSGDKKVNGLNFTRNRGKRVLAEVLIPRDVVEKHLHT
                     TPERIARLSQLAMVSSLHAHSFGSQAHYANVLAAVFIATGNDAACVAESAVGVTTFTV
                     EGNDLQATVTLPGLMIGTVGGGTRLPTQQTCLEIMDCAGPRKAKKFAEILAAGVLAGE
                     ISLTAAMAADEFATAHARYGRKLPGTEPKR"
     gene            1441..>1768
                     /locus_tag="PKO06_19495"
     CDS             1441..>1768
                     /locus_tag="PKO06_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71898.1"
                     /translation="MSLNVVLVTSRVTYVRDNYRAALERVLRINSSDQDIRIKALVLL
                     QIPATVLLKNIVGLPLIGAPQMAFALSRNWAESVLNDPRVQAADRAGVPVFRCASMNA
                     PEALTWL"
BASE COUNT          336 a          513 c          536 g          383 t
ORIGIN      
        1 tgcgctcttt gaaaaagtcc gcgatgtgtg tgcacgtttc ttgaatgttc cacccgagtc
       61 tattacgcct cagaagcgtc tggtagagtt tctgcaactg gactctctcg gcctcgttgc
      121 tctgctggct cactttcagg aagtctttcg ggtcagcctg cgcgatgtcg atctggccac
      181 tctgcgcgat ttttccgcgg tctgccatct catcatgcgc aagggaaact gggaggagtt
      241 gtgcgatctg aagacggccg gactgacgag cgatgccatg ccgccgctgc tcgactatag
      301 cgaagacggc atcaagcgtc gtcgcaagtt tctgaagaat cgccacggga tcgatccatc
      361 cgtctttggt caggtggcca atgccgatga cctcaacggg aacatcgagg gctttgtcgg
      421 atacagcatg gtgccgatcg gcatcgccgg cccgctcaag gtgaacggcg agtatgcccg
      481 cggagacttc ttcctgcccc tggccacgac cgagggtgct ctggtcagtt cgattgctcg
      541 cggtacgcag atcatcacta tgtctggtgg agcccggacg cgagtgctgg ccgattctct
      601 ggtgcgagcg ccggtctttc tgttcagcac gctcgacgag ctggcgtcgt tcaaaacctg
      661 gatcgatgcc aatctcgact tgattcgtga ggcggctgag aagaccacgc gccatggcaa
      721 gctgctcgat atcgagaact acccgatggg cacgaccatg tgtctgcgtt tcgtgtattc
      781 aaccgctgac gcgtccggtc agaacatgac cacgattgcg actcacgcgg cgatggatta
      841 catcaagaaa gcgtttccgg gcaagatggt cgactgcttc cttgaagcca acatgtccgg
      901 tgacaagaaa gtgaacggct tgaacttcac gcgcaatcgc ggcaagcggg ttctcgccga
      961 ggtgctgata ccgcgagatg tcgtcgagaa acacctgcat acgacacccg agcgcatcgc
     1021 gcgcctgtct cagctggcga tggtcagctc actgcacgcg cattcctttg gctcgcaggc
     1081 acattacgcg aatgtgctgg cggccgtctt tatcgcgacg ggcaacgacg cggcctgtgt
     1141 cgccgagtcg gctgtcgggg tgaccacgtt caccgtagaa gggaatgacc tgcaggcaac
     1201 ggtgaccctg cccggcctga tgatcggcac cgtcgggggt ggtacgcgcc tgcccacgca
     1261 gcagacctgc ctcgagatca tggattgcgc aggtccccgc aaggcgaaga agtttgccga
     1321 aattctcgcc gccggagtgc tggcggggga gatctctctg accgcggcta tggcagccga
     1381 tgagttcgcg accgcgcatg cccgctacgg ccgcaagctg cccggcacgg agccgaaacg
     1441 gtgagcctga acgtcgttct ggtcacttcc cgcgtaactt atgtgcgcga taactatcgc
     1501 gccgctcttg aacgggtatt acgtattaac tcatctgatc aggatattcg cattaaggct
     1561 cttgtactgt tgcagatacc ggcaacggtc ttgctgaaga acatcgttgg cttgcctctg
     1621 attggtgctc cacagatggc gtttgcactg agccgcaact gggcagagtc ggtgctgaat
     1681 gatccgcgcg tgcaggccgc cgaccgtgcc ggtgtgccgg tgttccggtg tgcgagcatg
     1741 aatgctccag aagcgctcac ctggctca
//
LOCUS       DAORHD010001146         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_87997_length_1768_cov_3.253357, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001146 DAORHD010000000
VERSION     DAORHD010001146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87997_length_1768_cov_3.253357"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKO06_19500"
     CDS             <1..320
                     /locus_tag="PKO06_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV71899.1"
                     /translation="VAEELDLRGMRVEEALHRTEEYLKNAEAMKLPKVHLIHGKGTGA
                     LQRSIHEFLKRSPWKNKFRFGRYGEGDLGVTVVVFDPASDPAPTADNQPTFGSRARKG
                     TKR"
     gene            403..>1768
                     /gene="rpoN"
                     /locus_tag="PKO06_19505"
     CDS             403..>1768
                     /gene="rpoN"
                     /locus_tag="PKO06_19505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNV71900.1"
                     /translation="MDNSLKLGLSQKLTQTLKMTPEMQLAIKILQLNSLELQNQIQEI
                     IETNPVLELDDKEPSSTEVSLEATAPITGEPVPPSKEFTDETRQDFGIDWQRYLQDSE
                     NTEFRVSAGSYSDDEETNYENFTTKKENLHEYLERQLHEASLNATEKKIGEYLIGLID
                     RNGYLRQTREQIADLLKIEITIVDKVIKAIQSMDPPGVGAYDLRECLLIQAREEGYKD
                     DVLTTVIDRHLPDLATNKIAQIAKEHGYEIEDVMAAAETIKQFNPKPGAAFPGSGSEI
                     YVTPDVFIEKEDGEYKVTLNERNLPRLRISPLYRDTMKNRNLSDKAAYEFIKTKLEAA
                     RSFLRYVEKRKDTIMNVTKAIIEVQKDFLDFGILHLKPLTLQDVAQVTGVHESTVSRA
                     TSGKYAQTPRGLFELKFFFSGGARTQTGEDVSTASVKQRIDNLVRSEDPRNTLSDSDI
                     VNRLK"
BASE COUNT          487 a          497 c          454 g          330 t
ORIGIN      
        1 ccgtcgccga agaactcgat ctacgcggga tgcgcgtcga agaagcattg catcgtaccg
       61 aagagtattt gaagaatgcc gaagcgatga agcttcccaa agtgcatttg attcacggga
      121 agggaacggg cgcactgcaa cggtcgattc acgagtttct caaacgctcg ccgtggaaga
      181 acaagttccg cttcggccgc tacggcgaag gggatctcgg cgtaaccgtc gtcgtcttcg
      241 atccggcttc agatccggcc ccaaccgctg acaatcagcc gacgttcggc tcacgggccc
      301 ggaaaggaac gaaacgatga tcgcaaattc ccctgctgaa ttctgacacg aactagtgta
      361 gaatagccgg tggatacatc ttcgatcatc taggccaggg gtatggacaa ctcactcaaa
      421 cttgggctca gccaaaagct gacgcaaacc ctgaaaatga cgccggaaat gcagctggcg
      481 atcaagattc tccagctcaa ttcgctcgaa ctgcagaacc agattcagga aatcatcgaa
      541 accaaccctg ttctcgaact cgatgataaa gagccgagtt cgaccgaggt ctcacttgag
      601 gcaacggcgc ccatcactgg cgaaccggtg cccccatcga aagagttcac cgatgaaacc
      661 cggcaggatt tcggcatcga ctggcaacgc tatctccagg attccgaaaa caccgagttt
      721 cgcgtctctg caggcagcta ctctgatgat gaagaaacga actacgagaa cttcaccacc
      781 aaaaaagaga atctgcatga gtatctcgag cggcaactgc acgaagccag tctgaacgcc
      841 accgagaaaa aaatcgggga atatctgatc gggctcatcg accgcaacgg gtatctgcgt
      901 caaacgcgtg agcagatcgc cgatctgctg aagatcgaaa tcaccatcgt cgacaaagtg
      961 atcaaggcta ttcagagcat ggatccccct ggcgttggtg cgtatgacct gcgcgagtgt
     1021 ctgctgatcc aagcccgaga agagggttac aaagacgacg ttctgacaac cgtgattgat
     1081 cgtcaccttc ccgacctggc cacgaataaa atcgcccaga tcgccaagga acacggctat
     1141 gaaatcgaag acgtcatggc ggcagccgag acgatcaagc aattcaatcc caagccgggt
     1201 gcggcttttc ccggctcagg gtccgaaatc tacgtcacac cagacgtctt catcgaaaaa
     1261 gaagacggcg aatacaaagt aaccttgaat gaacggaacc tgccgcgtct gcgcatcagc
     1321 cctctctata gagacaccat gaagaaccgg aacctctcgg acaaagccgc ctacgagttc
     1381 atcaagacca agctcgaagc cgctcgctcc tttctgcgct acgtcgagaa gcgcaaagac
     1441 acgattatga acgtgacgaa agccattatc gaggtgcaga aagactttct cgatttcggc
     1501 attctgcact tgaaacccct tactcttcag gatgtcgcgc aggtgacggg agtgcacgag
     1561 tcgaccgtca gtcgtgcaac aagcgggaag tatgcccaga caccgcgggg gctgtttgaa
     1621 ctgaagttct tcttctcggg cggggcacga acacagacag gtgaagacgt ttccactgcg
     1681 tcggtcaagc aacgcatcga taatctcgtg cgctcagaag accctcgaaa tacgctctca
     1741 gacagcgaca tcgtcaatcg cctcaaag
//
LOCUS       DAORHD010001147         1765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_88251_length_1765_cov_3.388889, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001147 DAORHD010000000
VERSION     DAORHD010001147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88251_length_1765_cov_3.388889"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PKO06_19510"
     CDS             complement(<1..580)
                     /locus_tag="PKO06_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71901.1"
                     /translation="MRLPFRRIRDRTLALVFVASLLPVILLGIFVGARLKNELTVQAL
                     RTQERVAEAIRQGIFIQLRAYSRQLLRLASEPDVQSLDVDRLRNTIFRFLDQNSSFFS
                     VFVYKPDGTVQTLAYRNRFAGDDSLIGSSVLDPKQERLRAVSDAFRKVVATREIVVNE
                     SISFSGQRTELLVLLPVRSFHDPQGLAGVISCG"
     gene            740..>1765
                     /locus_tag="PKO06_19515"
     CDS             740..>1765
                     /locus_tag="PKO06_19515"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV71902.1"
                     /translation="MTEKYEFQALQAKWHPIWNEKNLYRTSEDKGKPKFYCLDFFPYP
                     SGNGLSVGHLRNYVPTDVVVRMKRMQGFNVLHPMGWDAFGLPAENFAIQQGVHPSITT
                     AKNAANYRRQLTLVECSYDWSREVNSTDPKYYKWTQWLFLLLHKRGLAYQSLGAQWWC
                     PSCKTILANEQVEQGTCWRCESEVTKKDLKQWYFKITDYADRLLEDLQTIDWPDKIKK
                     MQENWIGKRVGTEVTFKAEHPTQAGKTFDFSIFTTRVDTIFGVTFMTLAPEHPLVADL
                     THPDRKAEVERYVNESRKKSEIDRLATDKEKTGCALGSFAINPFNGERVPVFVGDYVL
                     VSYGTGAV"
BASE COUNT          451 a          505 c          489 g          320 t
ORIGIN      
        1 ggccgcaact gatcactcct gccaacccct gcggatcgtg gaaggagcga accggtaaca
       61 aaaccagcag ttccgtgcgc tgacccgaaa atgaaatgct ctcgttgacc acgatctcac
      121 gggtggccac caccttgcga aaagcgtcgg aaaccgcccg caggcgttcc tgcttcggat
      181 cgaggacgga agatccaatc aatgaatcgt caccggcgaa ccggttgcga taggcaagcg
      241 tctgaacagt accatctggt ttgtatacaa aaacgctaaa gaagctggag ttctgatcaa
      301 ggaagcgaaa tatcgtgttg cgcagtcgat caacatccag agattgaaca tcgggttcgg
      361 aggccagacg gaggagctgt cgcgaatagg ctcgcaattg aatgaagata ccctgtcgaa
      421 tcgcctcggc cactcgctcc tgcgtgcgca gcgcttgaac cgtcaactca ttcttcagac
      481 gggcaccgac gaagataccg agcagaatga cgggcaacag gctcgcgaca aagacgagcg
      541 ccagtgttcg gtcacggatt ctgcgaaacg gtagcctcat gcccggcact atacacgatc
      601 aggcaacggc atcgcaaaac ctgagcggcc acacgtgata caagtgccgc ctgcgcctgc
      661 accagcatcc gacgttgaca atctccgggg gtggtactat gatgttcgca ctcatcctgt
      721 caggattctg gagaccctca tgaccgaaaa atacgaattt caggcactgc aagccaagtg
      781 gcacccgatc tggaacgaga agaacttgta ccgcaccagc gaagacaaag ggaaacccaa
      841 gttctactgt ctcgactttt ttccgtatcc gtccgggaac ggcctctccg tcggtcatct
      901 gagaaactac gtgcccaccg atgtcgtcgt gcggatgaaa cgcatgcagg ggttcaatgt
      961 cctgcacccc atgggctggg acgccttcgg tctcccggca gaaaactttg ccatccagca
     1021 gggagtgcat cccagcatca ccaccgcgaa gaacgccgcc aactaccggc ggcagctgac
     1081 gctggtggag tgctcctacg actggagccg ggaggtcaac tcgaccgacc cgaagtatta
     1141 caaatggacg cagtggcttt tcctgctgct gcacaaacgt ggtcttgcgt accagtcact
     1201 gggagctcag tggtggtgcc caagctgcaa gacgattctg gcgaatgaac aggttgaaca
     1261 gggaacctgc tggcgttgcg aatcggaggt caccaagaaa gatctcaaac agtggtattt
     1321 caagatcacg gactacgccg atcgcctgct tgaagatctg caaaccatcg attggcccga
     1381 caagatcaag aagatgcagg aaaactggat cggcaaacgc gttggcacgg aagtaacgtt
     1441 caaagccgaa catccgaccc aggccggaaa gaccttcgat ttcagcatct tcaccacccg
     1501 cgtcgatacg attttcgggg taacgttcat gaccctggca ccggaacacc cattggtggc
     1561 cgacctgacg caccccgacc gcaaggccga ggtcgaacgc tatgtcaacg aatcgcgcaa
     1621 aaagagcgag atcgaccgac tggcgaccga caaagagaag actgggtgcg cgctgggaag
     1681 cttcgcgatc aatcccttca acggcgaacg agtccctgtc tttgtgggcg attacgtgct
     1741 ggtgagctac ggcaccggag cggta
//
LOCUS       DAORHD010001148         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_88407_length_1763_cov_3.461358, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001148 DAORHD010000000
VERSION     DAORHD010001148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88407_length_1763_cov_3.461358"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKO06_19520"
     CDS             complement(<1..532)
                     /locus_tag="PKO06_19520"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV71903.1"
                     /translation="MSGPGHRREIADLILVLLVGLISGIAARSLGQPLFLGYLFAGVV
                     VGPHTGGVTVSDIPQIEQLADIGVALLLFSLGLEFTLKDLQPIRRVAIWGSCLQVALT
                     FVWAYAIGRLVQWPSEASLWFGTACVSSSTAVILKTLSDRGHLKTLSGRVMLSMSVVQ
                     DLLVIPIMILVGSLLKS"
     gene            complement(493..1044)
                     /locus_tag="PKO06_19525"
     CDS             complement(493..1044)
                     /locus_tag="PKO06_19525"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HNV71904.1"
                     /translation="MNEVRRTTGGIVQVVIIVVALMISMVACAEETASMAAPLSRLPA
                     TDTGEVRLSLGQTVYVPIYSQIYHGDLPSEFLLTGVLSIRNTDSRHAIQILSATYYDS
                     AGRVVREYLPRPIRLAPLSATQCLVPESDSAGGVGAAFLVRWKSQVAVNQPIMESVMI
                     STRSQQGLSFISRGQVIAERLPT"
     gene            complement(1193..>1763)
                     /locus_tag="PKO06_19530"
     CDS             complement(1193..>1763)
                     /locus_tag="PKO06_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71905.1"
                     /translation="KLAKRFGILTPYTSYLVLEDNAAIPGPRLRQALGPMDDMGGRFE
                     EERSRFSPQSLKSESTGANAFDSAVEMKAMRESAAPAPSMSAGGFSGRIRGSTESDKV
                     LSKLVTRDVEDRTFYLIDGRWEDSRIKESKADVTVKAYTAAYFELIKRYPEVRKFLAI
                     GEKVLVAIGDKVVEISPDEGVSDAGALKL"
BASE COUNT          418 a          561 c          450 g          334 t
ORIGIN      
        1 cgctcttcaa cagactgccg acgagaatca tgatcggaat gaccagcaga tcctgaacca
       61 ccgacatgct caacatcacc cgaccggaaa gcgttttcag atgtccgcga tcagacagtg
      121 tcttcaggat gactgccgtg ctcgacgaca cgcaggccgt gccgaaccac aatgacgctt
      181 ccgaaggcca ctgcaccaga cgaccgatgg catacgccca gacgaaggtg agcgctacct
      241 gcagacaact cccccagatg gccacgcgtc gtatcggctg cagatccttg agcgtgaact
      301 ccaacccgag cgagaacaac agcaacgcca ctccaatatc cgccagttgc tcgatctgag
      361 gaatgtcgga aacagtcacg ccgcccgtgt gcggacccac caccaccccg gcaaacagat
      421 agccgagaaa cagcggttgt cccaggcttc gggccgcaat accgctgatc agcccgacca
      481 gcagaaccag aatcaggtcg gcaatctctc ggcgatgacc tggccccgag atatgaacga
      541 caacccctgc tgactgcggg tgctgatcat caccgactcc atgatcggtt gattgaccgc
      601 cacctgcgac ttccaacgca ccagaaatgc cgcgcctacc ccgccggcgg aatccgattc
      661 cggaaccaga cactgcgtcg ccgacagcgg tgcgagacga atcggtcggg gaagatactc
      721 gcgaacgact ctgccggcag agtcgtaata ggtggccgac agaatctgaa tcgcatgacg
      781 cgagtcggtg ttgcgaatgc tgagaacgcc ggtcaacagg aattcagacg gcagatcacc
      841 atgatagatc tgcgagtaga tcggcacata tactgtctgc ccgagcgaca accgcacctc
      901 gccagtgtct gtcgccggca accgagagag cggagcagcc atcgaggccg tttcttccgc
      961 acatgccacc atcgagatca taagagcgac aacaataatt acgacttgta ctattccgcc
     1021 ggtcgtccgt cgcacttcgt tcatcacacg ctcctttttc tcaccagtat cggtcgtttt
     1081 tgtctgggga gggttctcac ataagatacc gaaaaaccat ctccgtgtac agcacctccg
     1141 gaactgaaaa aaggcggggt tgcccccgcc ttttcacctg tcgaggaacc gactacagtt
     1201 tcagagctcc agcatcactg acaccttcgt cagggctgat ttcaacgacc ttgtcgccga
     1261 tagcaaccag gactttttca cctattgcca ggaacttgcg cacttcggga taccgcttga
     1321 tcaactcgaa ataggcggct gtgtaagctt tgaccgtcac gtcagccttg ctctccttga
     1381 tccggctatc ttcccagcgg ccatcgatca ggtagaaggt acgatcttcg acatcgcgcg
     1441 tcaccagctt gctcagaacc ttgtccgatt cggtcgaacc gcgaatgcgc cctgagaaac
     1501 cacctgcact catcgaaggg gccggagccg ctgattcgcg catggccttc atctcgacgg
     1561 cagaatcaaa ggcattcgct cccgtcgact cgctcttcaa actctggggc gaaaagcgtg
     1621 atcgttcctc ttcgaaacgc cctcccatgt catccatggg gccgagagcc tgacgcagac
     1681 gaggtccggg aatcgcggca ttgtcttcca gcaccagata cgacgtatac ggcgtcagaa
     1741 tgccgaaccg cttggccagc ttg
//
LOCUS       DAORHD010001149         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_88705_length_1759_cov_3.553991, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001149 DAORHD010000000
VERSION     DAORHD010001149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88705_length_1759_cov_3.553991"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKO06_19535"
     CDS             <1..402
                     /locus_tag="PKO06_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71906.1"
                     /translation="KLFVYPEPMLSTDMLYVPKVREGLLRAKNLLPIVPAAVTPEQAE
                     TIWREMARLVAKAGNDVGMTARMVGYWDFPAWKLRFAVKYFQVAAKVAVDSEWEKTGG
                     DAQVWQKACEKLGERVNQLKTEIDEFLATLK"
     gene            complement(468..1562)
                     /locus_tag="PKO06_19540"
     CDS             complement(468..1562)
                     /locus_tag="PKO06_19540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV71907.1"
                     /translation="MKVALHLTHDCNLRCTYCYVGEKYGVRMSRETAEAAVRFAHQQS
                     RTYIDINFFGGEPLLEKDLLWHVIDYAHQYRLEQTETRPIRFFTTTNGVAIDEAFMKR
                     SVDHGLVLSLSLDGHAQGHDLTRTLPDGRGSFETIAANFPLILSHMPQIQVLMTQTPR
                     NVAWLAEGVEKLYHCGFRCFVLGPNFEEPWNVQAAEEWNKQIERIADFVIDRFRHGEF
                     ISISNFDAKIRSHTQTRAEVCSCCDKNDGEIAVAPSGNLYPCLRFVKEDRDHELLIGS
                     LRDGLDVRKRARIMAAASREWSDCLECGYRGRCFHYCSAVNFKLNGAFNQPPAFLCHT
                     EQAVIRQADRLAEILWQERNPEFTRRYYRG"
     gene            complement(1570..>1759)
                     /locus_tag="PKO06_19545"
     CDS             complement(1570..>1759)
                     /locus_tag="PKO06_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71908.1"
                     /translation="TANERGEMFALPLIGDCSKIAIGSRVVVQGTVTRRDGGPGEGKL
                     PQTYFVVRIEHIAPDNLR"
BASE COUNT          382 a          467 c          525 g          385 t
ORIGIN      
        1 aagctcttcg tctatcccga gccgatgttg tcaaccgaca tgttgtacgt gccgaaggtg
       61 cgcgaagggt tgctgcgtgc gaaaaacctg ttgccgatcg ttcctgcagc cgtgactccc
      121 gaacaggcgg aaaccatctg gcgtgagatg gctcgattgg ttgccaaagc tggaaatgac
      181 gttggcatga cggctcgcat ggttggctac tgggactttc ccgcctggaa gctgcgcttt
      241 gccgtgaaat actttcaggt tgccgccaag gtggcggttg acagtgaatg ggaaaaaacg
      301 ggtggagatg cccaggtttg gcagaaagcc tgtgagaagc tgggcgagcg ggtgaatcag
      361 ctcaagaccg agatcgatga gttcctggcg actctgaaat agaagctcgc acaaccaaca
      421 agcggaagac taatctttcg cttgttggtt tttttattgc tcgaaaatca accgcgatag
      481 taccggcgcg tgaactccgg attccgctcc tgccagagaa tttcagccag gcgatctgcc
      541 tggcgaatca ctgcctgctc ggtgtgacag agaaacgcgg gtggctggtt gaacgccccg
      601 ttcagcttga agttgaccgc gctgcagtag tgaaagcagc gaccgcgata cccgcattcc
      661 agacaatctg accattcgcg actggcagcc gccatgatgc gggcgcgctt gcgcacatcg
      721 agcccgtcac gtagagagcc gatcagcagt tcgtgatcac gatcctcctt cacgaaacgc
      781 agacagggat acagattccc cgacggggcc acggcgatct cgccatcgtt cttgtcacag
      841 cacgaacaga cttcggcgcg ggtttgtgtg tgcgagcgaa tcttggcatc gaagttcgag
      901 atcgaaatga actctccgtg gcgaaaacga tcaatgacga agtctgcaat tctctctatt
      961 tgcttgttcc actcttcggc agcctggaca ttccagggtt cttcgaagtt cggcccgagg
     1021 acgaagcagc gaaagccgca gtgatacagc ttctcgactc cttcggcgag ccaggccacg
     1081 ttgcgcggtg tctgggtcat gagcacctga atctgtggca tgtgactgag aatgagagga
     1141 aagttcgcgg cgatggtctc gaacgaaccg cgaccgtccg gaagcgtgcg cgtcagatca
     1201 tgtccctgcg cgtgtccgtc gagactcagt gagagtacca gcccgtgatc gacggaacgc
     1261 ttcatgaatg cctcatcgat ggcgacacca tttgtggtgg tgaaaaaacg gattggacga
     1321 gtctctgtct gctcgagacg atactggtgt gcatagtcga tcacatgcca gagcagatct
     1381 ttttcgagca gaggttcgcc cccaaaaaag ttgatgtcga tgtaggtgcg actctgttga
     1441 tgcgcgaaac gaacggcggc ttcggctgtt tcgcgtgaca tgcgtacacc gtatttttcg
     1501 cccacgtaac agtaggtgca acgcaggttg cagtcatgcg tgagatgcag agcgactttc
     1561 atcgccggtt cagcgcaggt tgtccggggc gatgtgctcg atgcgaacca cgaagtaggt
     1621 ttgtggcagc ttgccttcgc cgggcccacc gtcacgtcgg gtgacggttc cctggaccac
     1681 gacgcgcgat ccgattgcga tcttggaaca gtctccgatc agaggcagag cgaacatttc
     1741 gccacgctca tttgcggtc
//
LOCUS       DAORHD010001150         1759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_88726_length_1759_cov_2.668427, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001150 DAORHD010000000
VERSION     DAORHD010001150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88726_length_1759_cov_2.668427"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKO06_19550"
     CDS             complement(<1..254)
                     /locus_tag="PKO06_19550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71909.1"
                     /translation="MSDGFWVAVVAVFWIILMLIGNPKVKAWFQAELDRLRGIEPTKP
                     RAPHVPKPKPPDPIVEEHKTHIEEWPELPYPWVKHPELIDA"
     gene            513..956
                     /locus_tag="PKO06_19555"
     CDS             513..956
                     /locus_tag="PKO06_19555"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV71910.1"
                     /translation="MNLVKFFKDMEALQDQLSSLVSREFRRGPRMAFLPGESITDYPY
                     MNIAENADNVYVEALAPGVEPDSLKINVLRNTLTITGEKIPSKIPLEDHHRAERAPGK
                     FVRTVELPIEINSERVSAEYKNGLLFITLPKAEAAKPRQIEIKVN"
     gene            997..1401
                     /locus_tag="PKO06_19560"
     CDS             997..1401
                     /locus_tag="PKO06_19560"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV71911.1"
                     /translation="MSDKNTNTAVQADNTPNREATRNPEHYISPLVDIYETKDELCVV
                     AEMPGVDREGLNIRVENGILTIEGRMKTHELKDCTVRIREFRALNFFRQFELPEAIDQ
                     EKIRAELKHGVLSLTLPKQEKQKPRQIPISVG"
BASE COUNT          426 a          508 c          451 g          374 t
ORIGIN      
        1 gcatcgatca gttccggatg ttttacccat ggatacggca gttccggcca ctcctcgata
       61 tgcgtcttgt gttcttcgac gatggggtca ggcggcttgg gctttggcac atgtggtgca
      121 cgcggttttg tgggttcgat acctctgagt cgatcgagct cagcctgaaa ccacgccttc
      181 accttcgggt tcccgatcaa catcagaatg atccagaaaa ccgcaacgac tgccacccag
      241 aatccatctg acactgcata ccgcctttcg tgacctcgaa tccagctcaa cgcgaaacat
      301 gtcatcgtcg tcctatcgcc gtgcgttcat gatagaggaa gtagcgacgt cgcggcaacg
      361 tcgcgcgacg gtcgctcacg aaagccacaa agggttgttc cggccacaaa aaatcatata
      421 ccagcttcca gagcgggttt tcacaagttg gcacgcccat tgctaaccat gttgtcgttt
      481 gaagctcaac caaaatttcc aggaggtcca ttatgaatct ggtgaaattt ttcaaggata
      541 tggaagcact gcaggatcag ctgagcagtc tggtgtcgcg cgaattccgc cgcggcccac
      601 gcatggcgtt tctgcctggt gaatcgatca ccgactatcc ctacatgaac atcgccgaga
      661 acgccgacaa tgtctacgtt gaagcgctgg cgcccggggt cgaacccgac tcgctgaaga
      721 tcaacgttct gcgcaatacg ctcaccatca ccggcgaaaa gattccgtcg aagatccctc
      781 tcgaagatca tcatcgcgcc gaacgcgccc ccggcaaatt tgtccgcacc gtcgaactgc
      841 cgatcgagat caactctgaa cgtgtctcgg cggagtacaa gaacggcctg ctgttcatca
      901 ccctgccgaa ggctgaagcg gccaagcccc gtcagattga aatcaaagtg aactgacgcc
      961 atgaaccacc atcaccacca gaaggaggaa tcaaccatgt ctgacaagaa caccaacact
     1021 gctgtccagg ctgacaatac cccgaatcgt gaagcaacac gcaatcccga gcattacatt
     1081 tctccgctcg tcgacatcta tgaaaccaag gacgagttgt gcgtcgtggc cgaaatgccc
     1141 ggtgtcgatc gagaaggtct caacattcgt gtcgagaatg gcattctgac gatcgaaggt
     1201 cgtatgaaaa cgcacgaatt gaaggattgc accgtgcgca tccgtgagtt ccgcgctctc
     1261 aacttcttcc gtcagttcga actgcctgaa gcgatcgatc aggaaaagat tcgcgccgaa
     1321 ctcaagcacg gcgtgctgtc cctgaccctg ccgaaacagg aaaagcagaa gccccgccag
     1381 atcccgattt cggttggcta acccctcttc tctcccgggc ggttgccgac agatgccgcc
     1441 gcccgttccc gatacaagag aatcccccgg tcacgcgggg gattctgtct ttaagccaga
     1501 aatggtctcg gagtgatcct ccccctcaaa aatacttttc gaccagccag atgaacgcta
     1561 taatgagagt tgtctggggt tcacctgact ttccacagat tggggcgaag agaggaagag
     1621 cgaggtcatg agtatgtgga gcgcgattcg tcggtatgca gcaccgacgg tgctctgcgt
     1681 gctgattctg gtcatgattc gtctgagcag ttttgccgaa gaagccgaac gcggactgtt
     1741 gaacaacggc agctactat
//
LOCUS       DAORHD010001151         1757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_88875_length_1757_cov_3.616921, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001151 DAORHD010000000
VERSION     DAORHD010001151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88875_length_1757_cov_3.616921"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PKO06_19565"
     CDS             complement(<1..399)
                     /locus_tag="PKO06_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71912.1"
                     /translation="MKIPAYLPLERWDQRFGSKSDWYQTFYRQKEVVLSDALIESRLL
                     PRLLAFEQVKGGAIFGYAEQVRLVLEIGSRGRKGEHLVCLQPANIGWSRERLELSWKC
                     EVYNPQGKRLPAQEDEAIAGPLRDAVSALSL"
     gene            complement(502..1233)
                     /locus_tag="PKO06_19570"
     CDS             complement(502..1233)
                     /locus_tag="PKO06_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71913.1"
                     /translation="MTRHRVLWITLFLALAVWLTMPWLRMALNLLEQRMTGPKTIEDR
                     LIQYGTVVEQRLAPAFARVGLTFPPERVLLLGLKRERRLEVLVATASGPWHVLCAYPI
                     LAASGVSGPKLREGDRQVPEGLYRIESLNPNSRFHLALRVNYPNEFDRAQALRAGRDN
                     LGGDIMIHGNQVSIGCLAMGDEAAEDLFVLAARTGLPNIRVLLAPCDLRTEGLPPPAD
                     GDPAWLSELYKELQAEMKILPEMAR"
     gene            complement(1262..1576)
                     /locus_tag="PKO06_19575"
     CDS             complement(1262..1576)
                     /locus_tag="PKO06_19575"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV71914.1"
                     /translation="MKYTLDQAQTVLETPTARGRKLATEFDLEAVHLELSPGGAIPDH
                     ALDVPVAFYVVSGRGRLAVDGIESEAECGSWLTVPPKARRRWNNHGSELLTLLVVKQL
                     RS"
BASE COUNT          358 a          564 c          481 g          354 t
ORIGIN      
        1 cagagacaac gcgctcaccg cgtcgcggag cgggccggca atggcttcat cttcctgtgc
       61 cggcaggcgt tttccctggg gattgtagac ttcgcatttc cagctcaact cgagccgttc
      121 ccgactccac ccgatgttcg cgggttgcag acacaccagg tgctctccct tgcgaccgcg
      181 agagccgatt tcgagcacca gacgcacctg ctccgcatag ccgaagatcg cccccccttt
      241 cacctgctca aaggccaaca ggcgtggcag cagtcgactc tcgatcaacg cgtctgagag
      301 cacgacttcc ttctgacgat agaaggtctg gtaccagtcg ctcttcgagc caaaacgctg
      361 gtcccaacgt tccaggggaa gataggcggg aatcttcatc gtgactgcct ccgtttcaac
      421 gggtcttgcc aatatatgag tcgcttctag tatctcgcac tttcgtcatc cggaaaagtc
      481 tttcgccggc gttttctttt ctcaacgcgc catctcaggc agaatcttca tttctgcctg
      541 aagctctttg tacaattcac tcaaccaggc aggatctccg tcggcgggcg gcggcagtcc
      601 ttccgtgcgc aggtcacagg gggccagcag aacgcgaatg ttgggcaagc ccgtgcgcgc
      661 tgccagaacg aacaggtctt ccgctgcttc gtcacccatg gcgagacacc cgatcgacac
      721 ctgattgccg tgaatcatga tgtcaccgcc caggttgtca cgaccggcgc gcagagcctg
      781 ggcgcgatcg aactcgttcg gatagttcac ccgcaaagcc agatgaaatc gactgttcgg
      841 gttcagactc tcgatacggt agagcccctc gggcacctgc cggtcgcctt cacgaagctt
      901 cgggccgctg acaccgctcg cggcaagaat cggatacgca cacagaacgt gccaggggcc
      961 actcgctgtt gccacaagca cctcgagacg ccgttcacgt ttcagtccaa gcaacagcac
     1021 gcgttcaggg ggaaacgtca gccccactcg tgcaaatgcc ggagccaagc gctgttcaac
     1081 aacagtccca tactggatca gccgatcttc aatggttttc ggtccggtca tccgttgctc
     1141 caaaaggttc aaggccatgc gcagccaggg cattgtcagc cagacggcca gcgccaggaa
     1201 gagggtgatc cagagcactc gatgccgcgt catgcacgtg tgggccttgt cggttgcgcg
     1261 ctcatgagcg cagctgcttc accaccagaa gcgtcagcag ctcagagccg tgattgttcc
     1321 agcgacgtct ggcctttggc ggcaccgtca gccacgaacc gcactcagcc tctgattcaa
     1381 tgccgtcgac cgcgagacga ccacgaccac tcacgacgta gaatgcgacc ggaacgtcga
     1441 gcgcatgatc cggaatcgcc ccaccgggcg agagttccag atgaaccgct tcgagatcga
     1501 actcggtggc gagcttccga cctcgtgccg tgggagtctc aagcaccgtc tgcgcctgat
     1561 ccagggtgta tttcatcaac gacttccttt catcgcaatc gaaaacatgg aagacacgga
     1621 ttcctgtgct ataataccgc tctgagccgt atgcttccag ccattgagaa gatctttcca
     1681 tgcctgctcg acctgcttgc cggggacgac tacgcgttgc gccgggcggc gttgatcgcc
     1741 ctgagtcgcc tgcaggg
//
LOCUS       DAORHD010001152         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89209_length_1753_cov_3.353946, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001152 DAORHD010000000
VERSION     DAORHD010001152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89209_length_1753_cov_3.353946"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKO06_19580"
     CDS             <1..760
                     /locus_tag="PKO06_19580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV71915.1"
                     /translation="IAPADMEKTLCTIGTETIKIGAFLPELERVPPMFRAQLIDDFVG
                     QFCDRELVRRHVQQNIESMTKQYPEAVKAARRRVGVRLLLEENISKKVQVSDEDIKDF
                     YTKNLTQFAKPEEVRAHHILVEKEERAKELLATIEKKEKTFEDVAQADSKCPSGKRGG
                     DLGFFSKGQMVKEFDEAAQSAEIGKVVGPVKTEFGYHLIRVDERKASGTAALDEVKDF
                     IKQQLLPDRQRKALEEYVAELKKAYPVSEHPEKL"
     gene            936..>1753
                     /locus_tag="PKO06_19585"
     CDS             936..>1753
                     /locus_tag="PKO06_19585"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNV71916.1"
                     /translation="MEVIYLIMLAVGAAVGAAIGYFYQKNADRQAAEGILSSAEAQKR
                     QMIEKAQTEAQAKVEEAEKRAREIKEKESVIRKNTEREAKKRKHELDKLEAQLLSKIE
                     KNEARSDLLEKRADAMKAKEDAIEKLKQEKEKVLEEQKRKLEQVAGLSPEQAREQLLK
                     RVEEELQYEIAVKIKEAENRVKDAADKKSRDILATSIQRCSTDHSIDPIVTVVELPSE
                     EMKGRIIGREGRNIRSFEAITGVDVIIDDTPEAVVLSSFDPIKRAIARTTLDMLT"
BASE COUNT          480 a          437 c          527 g          309 t
ORIGIN      
        1 aatcgctcct gccgatatgg aaaaaaccct gtgcacgatc ggaacggaaa cgatcaagat
       61 cggcgcgttc ctgcccgaac tcgaacgtgt gccgccgatg ttccgggcgc agctgatcga
      121 tgactttgtc ggtcagttct gtgatcgtga actggtccgc cggcatgtgc agcagaatat
      181 cgagtcgatg accaaacagt atcccgaggc ggtaaaagcg gctcgtcgac gggtgggcgt
      241 gcgtctgctg ctcgaggaga acatctcgaa gaaagtgcag gtgtctgacg aagacatcaa
      301 ggatttttac accaagaatc tgacccagtt tgcgaaaccc gaagaagtgc gggcgcatca
      361 catcctggtg gaaaaggaag agcgcgcgaa agagctgctg gccaccatcg agaagaagga
      421 aaagacgttc gaagacgtgg cacaggctga ttccaagtgc ccctccggca aacgcggtgg
      481 tgacctcggg ttcttctcga aaggccagat ggtgaaggaa ttcgatgaag cggcacagtc
      541 ggccgagatc ggcaaggttg ttggaccggt gaagaccgaa ttcggatacc atctgattcg
      601 cgtcgatgaa cggaaagcct ctggaaccgc tgctctcgat gaagtcaaag acttcatcaa
      661 acagcagttg ttgcctgatc gacagcgcaa agcgctcgag gaatacgtcg ccgaactgaa
      721 gaaagcgtat ccggtttctg agcacccgga aaagctgtaa gagagtctgc ggctcggcgc
      781 gtcagcgccg agccgccatt caaggaaagg aaatcgctcc cggtcttttg agactgtgag
      841 ctgcactcac tgaacgcatg tcagccaaca aaccatcgtc gcctctcggg gcagatgatt
      901 caatcgatat atcaggaggt tcgcaaggtt cggtcatgga agtcatttat ctcatcatgt
      961 tagcggttgg agccgcggtt ggagccgcga ttgggtattt ctatcagaaa aacgcagacc
     1021 gccaggcggc ggaaggcatt ctgagcagcg cagaggcgca gaagcgccag atgatcgaaa
     1081 aggcgcagac tgaggcccag gccaaggtcg aagaggctga aaagagagcg cgcgagatca
     1141 aggaaaaaga gtcggtgatt cgcaagaaca ccgagcgtga agcgaagaag cgcaagcacg
     1201 agctcgacaa actcgaagct cagctgttgt cgaaaatcga aaaaaacgaa gcccgttctg
     1261 atctgctcga aaagcgggct gatgccatga aagccaagga agacgcgatc gagaagctga
     1321 aacaggaaaa agaaaaggtg ctcgaagagc agaagcgcaa actcgagcag gttgccggac
     1381 tgagccccga acaggcacgc gagcagttgc tcaagcgagt cgaagaagaa ctgcagtatg
     1441 agatcgcggt caagatcaaa gaagccgaaa accgggtcaa ggatgcggcg gacaaaaagt
     1501 cacgcgatat tcttgcaacc tcgattcagc gctgttccac cgaccattcc atcgatccga
     1561 tcgtgaccgt ggtcgagttg cccagcgagg aaatgaaggg acgcatcatc gggcgtgaag
     1621 gtcggaacat tcgctccttt gaagcgatta ccggtgtcga cgtcatcatc gatgatacgc
     1681 ccgaagcggt cgttctctcg tcgtttgacc cgatcaaacg ggcgatcgcc cggacgacgc
     1741 tggatatgct gac
//
LOCUS       DAORHD010001153         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89222_length_1753_cov_2.928740, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001153 DAORHD010000000
VERSION     DAORHD010001153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89222_length_1753_cov_2.928740"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1753)
                     /locus_tag="PKO06_19590"
     CDS             complement(<1..>1753)
                     /locus_tag="PKO06_19590"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71917.1"
                     /translation="LSRLQGQAAADIILERYESDNLEDYLTVALSLIDPEKAAKALIA
                     ALSSPHIEARISAAEALIANPHEETIFALTSAVETYLNQSTGQQSGVSLVTEDALASA
                     VRALGAIKSPMSVPLLRKVITKEGSTRLRATAVASLSNHMVDSLVPMIQALLKDPDAR
                     VRANAIEALQCIDKPAIIGMLQPYLYDSHQRVRANAAKAIWKYGDYDVTSTLKEMLGH
                     KEKAQRVSGIYAIGEIKADTFVKNLLAYLKDPDADVRRNAVIALKKFGKRDFSMDLIA
                     LIDDPVPDIRVQAAAALAEVFQGSAQAQLLKRFSVETESAVRCALIGHLGHIGTTNAL
                     PSLMQALYDQDERVVITAIETIGRLQNERPATEMVQKLMPFLAVKSRQIRARAIRVLW
                     QWGCQEVLTTILDMLGESSVDNRLSALDILGELFAEISRNQLELRQECEACLQAAADH
                     LRQDLKKKTNSELETQIEGLWKESSTHIQNSDFDQARSILEHLLTLEPNHLQALIQLG
                     EIYLRDKDDFQAESCFTKALELNSNTVKAHYCLGQIFHGQQDWERASKSLQTAIRLYP
                     KLPQAYLWLADALEGISQ"
BASE COUNT          349 a          477 c          540 g          387 t
ORIGIN      
        1 gctggctgat gccctcgagg gcatcagcca gccacaaata ggcctgcgga agtttcggat
       61 acaagcgaat cgccgtctgc agcgacttgg acgctcgttc ccaatcctgc tggccgtgaa
      121 agatttgtcc caggcagtag tgggctttaa ccgtattgga attgagttcg agtgccttgg
      181 taaaacacga ctcggcctgg aaatcatcct tgtcgcgcag atagatctcg ccgagctgga
      241 tcaacgcctg cagatgattg ggttcaagtg tcagcaggtg ttcgagaatc gaacgagcct
      301 gatcgaagtc actgttctga atgtgcgttg aggactcttt ccaaagccct tcaatctgag
      361 tctcgagttc cgagttggtt tttttcttga gatcctggcg cagatgatcg gcggccgcct
      421 gcaggcatgc ttcgcactcc tgccgcagtt cgagctgatt gcgcgagatt tcggcaaaca
      481 gttcgccgag aatgtcgagc gcggaaagac ggttgtcgac gctgctttcg cccagcatgt
      541 cgagaatcgt ggtcaggact tcctgacatc cccactgcca cagcacgcga atcgcccgcg
      601 cccgaatctg tcggctcttg acggccagaa acggcatgag tttctgaacc atctcggtgg
      661 ctgggcgctc gttctgcaga cggccgatgg tttcgatagc cgtgatgacg actctctcat
      721 cctggtcgta cagggcctgc atgagagacg ggagcgcgtt cgtcgtgccg atgtgcccaa
      781 ggtgaccgat aagcgcgcag cgaacggccg attcggtctc gaccgaaaaa cgcttcaaca
      841 gctgagcctg cgccgagccc tgaaagactt cggccagggc ggcggcggcc tgaacgcgga
      901 tatccggaac cgggtcatca atcagcgcga tcagatccat ggaaaagtcg cgttttccga
      961 actttttcag agcgatgacg gcgttccgac gcacgtctgc atccgggtct ttgagatagg
     1021 cgagcaggtt cttgacaaag gtgtcggcct tgatttcacc tatggcgtaa atgcccgaga
     1081 cgcgctgggc cttttccttg tggccgagca tctctttcag ggtcgaagtg acatcatagt
     1141 cgccgtactt ccaaatggct ttggccgcat tggcgcgcac ccgttggtgc gaatcgtaca
     1201 gatacggctg caacatgcca ataatggcgg gtttgtcgat gcactgcagt gcttcgatgg
     1261 cattggcacg cactcgcgca tccgggtctt tcaacagggc ctgaatcatc ggcacgagag
     1321 aatcgaccat gtggttactg agagatgcga cagcggtggc acgcaggcgg gtgctgcctt
     1381 ccttggtgat gaccttgcgc aacagaggca cggacatggg gctcttgatg gcaccaagag
     1441 cgcgcaccgc gctggccaga gcgtcttcgg tcaccaggga aacgccgctt tgctgcccgg
     1501 tggactggtt gagataggtt tcgacggcgc tggtaagggc gaagatcgtt tcttcgtgcg
     1561 ggttggcgat cagagcctcg gccgcgctga tgcgcgcttc gatgtgcggc gaactgagcg
     1621 cggcgatgag ggctttggcg gccttctcag gatcgataag cgaaagagcg accgtgagat
     1681 aatcttcgag gttatcgctc tcgtagcgtt cgagaataat atcggccgcg gcttgcccct
     1741 gcaggcgact cag
//
LOCUS       DAORHD010001154         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89353_length_1751_cov_4.656250, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001154 DAORHD010000000
VERSION     DAORHD010001154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89353_length_1751_cov_4.656250"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKO06_19595"
     CDS             <1..528
                     /locus_tag="PKO06_19595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV71918.1"
                     /translation="RRQHVAATLLALTGLGLLAGEAGSADSDHRALLGCLLVLLPQFA
                     WAMYGVIGKSLIERISWPYLCRDTFALGSLMLLPFALVEGWWLGWGAWNFASVATLLY
                     LAIANSVCTYGLWNSALQDIPVSTASFILYVQPVSGALLSWWLFNEALGMRGACGSAL
                     IFVAMALVLFPSHKH"
     gene            663..1733
                     /locus_tag="PKO06_19600"
     CDS             663..1733
                     /locus_tag="PKO06_19600"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV71919.1"
                     /translation="MILSSSRGLHVLFLTFACLFMLCSRSAAQDYSTNQDFPTYNQDP
                     SAYASDLEETPNTSDYSNYGVDDGDDLSLPTEPPAAPGPAPVVPRPAINLAEKTILIG
                     RIPYLSLKDMMQQVRPLLAHLRKETGAKEVRLVTARNYAGVLEALSRDKIDFAWVGPT
                     AYVLARSRDQLMAVARAKHQSAVSYRGVFITRKNSGIQGLDDIKGKTIGFVDPESASG
                     YLYPLYVLHRLKINPHKSCRSVQFLKRHDVVLKAVLDGKIDVGVCLDATLTALADKTQ
                     LSQLLILGRTDEVPSDVICCRQDCPTNLREQVQAALLKISPTPSVATGSFPLPTFETA
                     TDDEYDYVQTVLQAVAPMAQHP"
BASE COUNT          327 a          552 c          460 g          412 t
ORIGIN      
        1 cgacgccagc atgtcgcggc gaccctgctg gcactgaccg gcctcggatt actcgccggc
       61 gaggccggca gtgccgactc agaccaccgg gcattgctgg gatgtctgct ggttcttctg
      121 ccgcaattcg cctgggcgat gtacggggtc atcggcaagt ctctgatcga gcgcatctca
      181 tggccgtatc tgtgtcgtga cacgttcgct ctgggttccc tgatgctgct tccgttcgca
      241 ctggtcgaag gctggtggct gggctgggga gcctggaatt tcgcctctgt cgcgacactg
      301 ctctatctgg ccatcgccaa ttccgtctgt acgtatggat tgtggaacag cgcactccag
      361 gatattcccg tatcaaccgc cagtttcatt ctctacgtcc aacctgtgtc cggcgctctc
      421 ctctcgtggt ggctgttcaa cgaagctctt ggcatgcgtg gggcatgcgg ctctgccctg
      481 atttttgtgg ccatggcctt ggttctattt ccttctcaca agcactaatt cacattttct
      541 gctgcactga ccccgtactt ttgtctgatt tgcatcgttg ttccaacctg accctcgatt
      601 ctctttccaa accgggagat tttgtttacc atgaggttca gcttagagac ggagcttcca
      661 ccatgatcct ttcatcatct cgcggcctgc acgtcctgtt tctgaccttc gcgtgtctat
      721 tcatgctgtg ttcacggagt gcggcacagg actattccac caaccaggat ttcccgacct
      781 acaaccagga tccgagcgct tatgcctctg acctggagga aaccccgaac acgtccgact
      841 acagcaacta cggggtcgac gacggagacg acctgagtct gccgaccgaa ccgcctgccg
      901 ctcccgggcc ggcgcccgtg gtaccacggc cagccatcaa tctggctgag aagaccattc
      961 tcatcggccg cattccgtat ctcagtctga aagacatgat gcagcaggta cgaccgctgc
     1021 tggcccatct gcgcaaggaa accggcgcga aggaagtgcg tctggtcacc gcccgcaact
     1081 atgcgggggt tctggaagcg ctttcacgcg acaaaatcga tttcgcgtgg gtcggtccga
     1141 ccgcgtatgt tctcgcccgg tcacgcgatc agctcatggc cgtcgcccgc gccaaacatc
     1201 agagcgccgt gtcctatcgc ggtgtcttta ttacccgcaa aaacagcggt attcaggggc
     1261 tcgacgatat caagggaaag accatcggtt tcgtcgatcc ggaatccgcc agcggctacc
     1321 tgtatcctct ctacgtgctg catcgcctca agatcaaccc gcacaagtcc tgccgaagtg
     1381 tacagtttct gaaacgacat gatgttgttc tgaaggcagt actcgacggc aagatcgatg
     1441 tcggcgtctg tctcgacgct accctgaccg ctctcgcgga caagacacaa ctcagtcagt
     1501 tgctgattct cggtcgcacg gatgaagttc cttccgatgt catctgctgc cggcaggatt
     1561 gcccgacgaa cctccgcgaa caggtgcagg ctgcgttact gaaaatctcc cccaccccgt
     1621 cggtcgccac cggctccttt cctctgccga cattcgaaac ggcgacagac gatgagtatg
     1681 actatgtgca gactgtcctg caggccgttg cacccatggc tcagcaccca tgagttcccg
     1741 tcacgactca t
//
LOCUS       DAORHD010001155         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89600_length_1748_cov_3.217956, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001155 DAORHD010000000
VERSION     DAORHD010001155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89600_length_1748_cov_3.217956"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKO06_19605"
     CDS             complement(<1..371)
                     /locus_tag="PKO06_19605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV71920.1"
                     /translation="MNVQHDTDGLNSPVIREALFADLDTIVEFNLRIARETEERELDR
                     RTVTRGVETLLREPARGRFWLAIVDGRPAGQIMVTYEWSDWLAADFWWIQSVYVHPDF
                     RRRGVYRSLHQHVEQEARRNGA"
     gene            complement(409..>1748)
                     /locus_tag="PKO06_19610"
     CDS             complement(409..>1748)
                     /locus_tag="PKO06_19610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71921.1"
                     /translation="MSIQESLPDRDGKPLYLVHAGFNHSQFERNFCLKHVNDLRRSET
                     LPIDLAMVATSKVVGGVYPHDLDRDYAAYLANLSTSNRSTVHEYRTEAGQNYVWIAMP
                     ARKMSYFFLVGRVPLHGVERKIQGMWHQLIVFAVLLLITSIFLGFMVARQFLRPINDI
                     STGLQAIARRDFRHRVPVRSADELGEVSSLINRVTEGMKDLEVARVVQESLFPTEPLE
                     VPPVRLFGRSFAMADIGGDYFDYFVLDDRNVVGLVGDVSGHGVSAALIMGMAKGAFAM
                     HAKPGLSLIAFFESFNHLLISTAKKKKMMTLFAFAIDVKTLKMEFLNAGHNYPMLYDA
                     ATGKVTELQAVGYPLGMRNKLASSVQVLQLHQGDGVCMYTDGYVESRNPAGEEIGYLR
                     LGDWFGVGIRRSTHVPSLVQYLFDEFHQWCGDEAVQDDVSLVCLKIDDGGIDP"
BASE COUNT          409 a          507 c          470 g          362 t
ORIGIN      
        1 gcaccgttgc gacgggcttc ttgttcgaca tgctgatgca gagagcgata gacaccccgg
       61 cggcggaagt ccgggtggac atacacgctc tggatccacc agaagtccgc ggccagccaa
      121 tcgctccatt cgtaggtgac catgatctgg ccggccgggc ggccgtcgac gatggcgagc
      181 cagaagcgtc cgcgcgcggg ctcgcgaagc agggtctcga ccccgcgcgt gaccgtccgc
      241 cgatcgagct cgcgttcttc ggtctcgcgg gcgatgcgca ggttaaattc gacgatcgtg
      301 tccaggtcag caaacaaggc ctcacgtatg acaggcgagt ttaaaccatc cgtatcgtgc
      361 tgaacgttca tatgttttcc tttcctggat ctcctgccgc cttcgcgatt acggatcaat
      421 acctccgtca tcgatcttca ggcacaccag cgacacatca tcctgcacag cctcatctcc
      481 gcaccactgg tgaaactcgt caaagaggta ttggaccaga gagggcacat gtgtcgatcg
      541 tcggatgccc acaccaaacc aatcgcccaa tctcaggtat ccgatttcct ccccggctgg
      601 gttccgtgac tccacatatc catccgtata catgcagact ccatcgccct gatgcagctg
      661 gagaacctgt acggaagagg caagcttgtt gcgcattccg agtgggtatc caaccgcctg
      721 caattccgtg acttttccgg tagcggcatc atagagcatg gggtagttgt ggccagcgtt
      781 gaggaattcc atcttcagag tcttgacatc gatggcaaaa gcaaacagag tcatcatctt
      841 cttctttttg gcggtgctga tgagcaggtg attgaatgac tcgaaaaacg cgatcagcga
      901 caacccaggc ttcgcatgca tggcaaacgc ccccttcgcc atgcccataa tcaacgcggc
      961 tgaaacgcca tgccccgaaa catcgcccac cagcccaacc acgttacgat catccaggac
     1021 aaagtaatca aagtaatcgc cgccaatgtc ggccatggcg aaactgcggc cgaacagccg
     1081 aaccggcggc acttcaagtg gttccgtcgg gaaaaggctt tcctgaacga cgcgggccac
     1141 ctcgagatcc ttcatgccct cggtcacgcg attgatcaga gaactgactt ctcccagctc
     1201 atcagcagat cgtactggta ctcgatggcg gaagtcgcgt ctggcaatgg cctgtaggcc
     1261 ggtagaaatg tcattgatcg ggcgcaggaa ttgtcgggcc accatgaaac cgagaaagat
     1321 gctggtaatg agcaacaaga cggcgaaaac gatcaactgg tgccacattc cctggatttt
     1381 tcgttcgacg ccatgcaaag gcactctgcc gaccagaaag aagtaggaca tctttctcgc
     1441 gggcatggcg atccagacgt agttttggcc cgcttcggtg cgatactcat gtaccgtgga
     1501 gcgattgctt gtggaaagat tcgccagata ggcggcataa tcacgatcca aatcgtgagg
     1561 ataaaccccg ccaacgactt tgctggtggc aaccatcgcc agatcaatcg gcagtgtttc
     1621 tgagcggcgc aggtcattga catgcttgag gcagaagttg cgctcaaact gcgaatggtt
     1681 gaatccggca tgcacaagat acagaggctt tccgtctcga tcaggcagcg actcctgaat
     1741 gctcatgg
//
LOCUS       DAORHD010001156         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89679_length_1747_cov_3.291962, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001156 DAORHD010000000
VERSION     DAORHD010001156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89679_length_1747_cov_3.291962"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1747)
                     /locus_tag="PKO06_19615"
     CDS             complement(<1..>1747)
                     /locus_tag="PKO06_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71922.1"
                     /translation="CLSLERDKPNPERENKLVHILETIPNLASFDQADWEYLSRQGPR
                     YLLLEVLTAMGRRPPYEALSVLEECLRHPDAGIRSAAVLPALFSNDMAVVSHSLALLN
                     DPCDQVAEEVFKQLRTCTGTELALLFDRGLRVQDQGILQSFAAFLPLIVREELRPLCL
                     RLQQHADPSLQQAASQALEALDLRNQDQQPITESSGESTPSVIPELEESPSLPTETTF
                     EPFAAIESVASLALTLPTMEIPRPELDPLIMAKPIAVPDFTALFRSLPPAPPPPPPPP
                     PPPAFLLASAIPERPPEKSAIDLIPDPPVPSTPAAKRTSHTPASAAPVKNVKTPETEH
                     AVATKPAVKPAALPSGSIKTEPWTPTREPVSAAQQPVATRPPAKERVIKLKGRLKSAD
                     TEPSATSEAPSQPPAVSAATAESLPPGRQTHATTTPPPPPPSDPGPQQSIPRQSVPSG
                     QVFAHPTPPEEDISFSIVEEASQANLPATPPSTELGTVSAKPEASSKTAEIQPASAPV
                     LTPAAAPATPAMPSQPATSPDPAAPGPLSAKPPANEPASIAPATGKAVSPKSSAPAGA
                     AAGTPPPSGTPAQIQD"
BASE COUNT          319 a          447 c          627 g          354 t
ORIGIN      
        1 tgtcctgaat ctgagcgggg gtgcccgaag gtggaggagt gccagcggca gcaccggcgg
       61 gtgcgctcga tttcggggaa accgctttgc cggttgctgg ggcgatcgaa gcaggctcgt
      121 tggccggggg cttcgcagat aacgggcctg gagcagcagg gtcaggggaa gttgcgggct
      181 gtgacggcat tgccggagtt gctggcgcgg cggcgggtgt gagcacgggg gcagaggcag
      241 gctgaatctc cgccgtcttc gacgaagcct ctggtttcgc cgagactgtg cccagctctg
      301 tggagggcgg cgtagccggc agattcgctt gcgaagcttc ttctacaatg gaaaacgaga
      361 tgtcctcctc gggcggagtc gggtgcgcaa acacctgccc gctgggcacg gattgtcggg
      421 gaatcgactg ttgcggacca ggatcagatg gcggtggtgg cggcggagtc gtggtggcat
      481 gcgtttgccg ccctggcgga agcgattcgg cagttgccgc agatactgca ggtggttggc
      541 tcggagcctc tgaggtggct gatggttccg tgtcagcgga cttcaaacga cctttcagct
      601 tgatgacccg ctctttggca ggtggtcggg tcgcgacggg ttgctgcgct gccgatacgg
      661 gttcgcgggt tggcgtccat ggctcagtct taatactgcc agagggtagg gcagcgggtt
      721 tgaccgcggg cttcgtcgcg accgcgtgtt ccgtttctgg cgttttgaca ttctttacgg
      781 gagctgctga tgctggcgta tgacttgtac gtttcgcggc aggtgtcgac ggtaccggcg
      841 ggtcgggaat gagatcgatc gccgatttct ccggtgggcg ttcaggaatg gcggaagcca
      901 ggagaaacgc gggcggtggt ggtggtggtg gtggcggtgg cggtgctggc ggcagactgc
      961 ggaacaacgc ggtaaaatcc ggcactgcga taggcttggc catgatgagc gggtcgagtt
     1021 ccggtcgtgg aatttccatg gtgggcaggg tgagagcgag agatgcgacc gactcgatcg
     1081 ctgcaaaagg ctcgaaggtt gtctccgttg ggagtgatgg tgactcctca agctccggga
     1141 tgacgctggg cgtggactcg cccgacgatt cggtgatcgg ctgctgatcc tgatttctga
     1201 gatcgagagc ttcgagtgcc tgcgacgctg cctgctgtaa agacgggtca gcatgctgct
     1261 gaagcctcag acagagcggg cgcagttctt cacggacaat caagggcaga aaagcggcga
     1321 aggactgcag aatgccttga tcctgaaccc gcaacccacg gtcgaaaagt aacgcgagct
     1381 cggtgccagt gcaggtgcgc aactgcttga aaacctcctc cgcgacctga tcacagggat
     1441 cattcaagag cgcgagagag tgcgagacaa ccgccatgtc attcgaaaac aacgcgggca
     1501 gaaccgccgc gctccgaatg ccggcgtcgg ggtgtcgtag acactcttcg agaaccgaca
     1561 acgcttcata cggaggccgt cgacccatgg ccgtcaggac ctcgagcaga agatagcgcg
     1621 gaccctgtcg actcaggtat tcccagtcgg cttgatcgaa acttgcgaga ttgggaatgg
     1681 tttcaagaat atggaccagc ttgttctcgc gctccgggtt cggcttgtcc ctttcgagac
     1741 tcagaca
//
LOCUS       DAORHD010001157         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89680_length_1747_cov_3.153073, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001157 DAORHD010000000
VERSION     DAORHD010001157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89680_length_1747_cov_3.153073"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1007)
                     /gene="dusB"
                     /locus_tag="PKO06_19620"
     CDS             complement(3..1007)
                     /gene="dusB"
                     /locus_tag="PKO06_19620"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNV71923.1"
                     /translation="MSSTRSLIHPFAIGGVPLTSNLILAPMAGVTDGPFRFLCQGLGV
                     GLTVSELASSRAILRGNRKTIEMLAFQAEIRPFAAQIFGADPETMAEAARRVEALGVA
                     DMIDINMGCPVAKVVKTGAGAALSKTPELASRIIRAVRNAVRLPVTVKFRLGWTDNTI
                     NVREFTRMAVDSGAAAICVHARTRQAMYSGAAQWHLLEGLQSLCGDIPFIANGDLTTG
                     DDLARVHEISGCSAFMIGRGALGRPWIFSELVQNKPSPGSDERYQIFREHLIEMLMEH
                     GHKGVPLFRVHLFSYLKGGANAAHLRKHLAEERDPGRVLELGRAFFQGQPLPVPSEAA
                     "
     gene            complement(1021..>1747)
                     /locus_tag="PKO06_19625"
     CDS             complement(1021..>1747)
                     /locus_tag="PKO06_19625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV71924.1"
                     /translation="FKSYDSTAHYEETIRTMICVPLKVRDRATGVINVVNKSGTDQFT
                     ADDQHLLEALAQQAAMAVEHARLYELAITDGLTKLFIHRYFQARLEEEILRARRYHTT
                     CSLLLFDIDHFKKFNDTYGHQQGDIVLMEVAKLIKQTVRDTIDIPARYGGEEFAIILP
                     ETDTAGAQLVAERMRKVVEMYDFPGQGKPLKVTISLGIATFPDHASTRPTLIKKSDIA
                     LYACKKRGRNCSIVYNESLDSLS"
BASE COUNT          372 a          483 c          538 g          354 t
ORIGIN      
        1 catcaggccg cctccgaagg aacgggaaga ggctgcccct gaaaaaaggc tcgaccgagt
       61 tccaggaccc gcccagggtc gcgttcttcg gccagatgct tgcgcagatg cgcggcgttg
      121 gcaccaccct tgagatagct gaagagatgc actcgaaaga gaggaacccc tttgtgtccg
      181 tgttccatca gcatttcgat aagatgttcg cgaaagatct gatagcgttc atcgctcccg
      241 ggagaaggtt tgttttgtac gagttccgaa aatatccaag ggcgtcccag ggctcctctt
      301 ccgatcatga aagcggagca gccgctgatt tcgtgcacgc gggcaaggtc gtcgccggtg
      361 gtcaggtctc cgttggcaat gaagggaatg tcgccgcaca gactctgcag gccttcgagc
      421 agatgccact gtgcggcgcc ggaatacatg gcctggcgag tgcgcgcatg cacgcagatc
      481 gctgccgccc cggaatcgac agccatgcgc gtgaactctc gaacgttgat ggtgttgtcg
      541 gtccatccca ggcgaaactt gacggtgacc ggaaggcgca cggcgttccg aacggctcgg
      601 ataattcgcg aagcaagttc cggagtcttc gagagagcgg caccggcacc ggtcttgaca
      661 accttggcga ccgggcaacc catgttgatg tcgatcatgt cagccacgcc cagcgcttcc
      721 acgcgtcggg cggcttcggc catggtctcc ggatcggcac caaagatctg cgcggcaaag
      781 ggacgaatct cggcttgaaa ggccagcatt tcgattgtct tgcggttgcc tcgcagaatc
      841 gcgcgcgaac tggccagttc agaaaccgtc agcccaacac ccaacccctg gcacagaaaa
      901 cgaaacggcc catcggtgac tccggccatg ggagcgagaa tcagattcga cgtgagtgga
      961 actcccccga tggcgaaggg gtgaatcagg gaacgcgttg aagacatgcg gaaacgccgc
     1021 tcacgaaagc gagtcaagcg actcgttata cacgatcgaa cagttgcgcc cccgcttttt
     1081 acaggcatac agagcaatgt cggacttctt gatcagggtg gggcgggtac tggcgtgatc
     1141 ggggaacgtg gcgatgccga gggagatcgt cactttgagc ggtttgccct gacccgggaa
     1201 atcgtacatc tcgacaacct tgcgcatgcg ctcggcaacc agttgtgcgc ctgccgtatc
     1261 agtttcaggc aggatgatgg caaactcttc gccgccgtag cgagccggga tgtcgatagt
     1321 gtcgcgcacg gtttgcttga tcagtttggc gacttccatg agcacgatgt cgccctgctg
     1381 gtgaccgtac gtatcgttga acttcttgaa gtgatcaata tcaaacagga gcagcgagca
     1441 ggtggtgtga tagcgtcggg cgcgcagaat ctcttcttcc agacgggcct gaaagtaacg
     1501 atggatgaac agtttggtca gaccatcggt gatggccagt tcatacaggc gagcgtgttc
     1561 aaccgccatg gctgcctgct gggcgagcgc ttcgagcaga tgttgatcat cggcggtgaa
     1621 ttgatcggtg ccactcttgt tcacgacgtt gatgaccccc gtggcgcggt cacgcacctt
     1681 gaggggcacg cagatcatcg tgcgaatcgt ctcttcgtaa tgagcggtgc tgtcgtagct
     1741 cttgaac
//
LOCUS       DAORHD010001158         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89933_length_1744_cov_3.786856, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001158 DAORHD010000000
VERSION     DAORHD010001158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89933_length_1744_cov_3.786856"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKO06_19630"
     CDS             complement(<1..562)
                     /locus_tag="PKO06_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71925.1"
                     /translation="MSQETTPVPESYLQISRAALWGMALGLVLFTGFAMTLCMKIDFP
                     LYFAEREVALKVGGTIIDLPTFRQLKDAAELDREKLSDQGFASELLDLLLLSEAGRKA
                     GIDRDAGFQKRIRDYDEAMAIASTTSVIQRTLFLFEELAEQTRQHLVGGAVASASATS
                     VPAMPASQPQRLHVREIVVVPATDSDK"
     gene            complement(562..1290)
                     /locus_tag="PKO06_19635"
     CDS             complement(562..1290)
                     /locus_tag="PKO06_19635"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV71926.1"
                     /translation="MPLLKNSDHSPGNLVHCTCLPTVLFRSDGVVLLVLFVFFALAQV
                     ATAAPEPFLAPGSHAPTTNIEDLTGTKHNVPATGSWSIVFFWSLFCHTCLDEIPHLIE
                     ETAHLASPSCTPFFISLDGSRMRKGLQNFQKKRRLEATLLVDEVASAAYLTADKWGVR
                     TTPSVFLVDPGGVIRYSREGPFDLQEILALMKGPTPASSNLSLSLPSSASDTQETLPA
                     STSLSAPASASEKVLSPSEPARGP"
     gene            complement(1268..>1744)
                     /locus_tag="PKO06_19640"
     CDS             complement(1268..>1744)
                     /locus_tag="PKO06_19640"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV71927.1"
                     /translation="ILTLPLLWLLGPDTPPSSLGSLEGVTLSTVNGEKFKLSGLFSDK
                     KILFVFWSITCATCIEEIPFIVHLHENWKDRLTIIGIHPPGFPLPKVQKFLRKFPTKI
                     PYQIAIDDESALMKAFQVTILPKVVLSDRRGQILYSHLGYDQSMETEIENAIAEKL"
BASE COUNT          412 a          434 c          543 g          355 t
ORIGIN      
        1 ctttgtcaga gtcagtggct ggtacaacca cgatttctcg cacatgcagc cgttgcggct
       61 gagaggcggg catggcaggc actgatgtcg cgctcgccga ggcaactgcc ccaccgacca
      121 ggtgttggcg cgtctgctcg gcgagctcct cgaacagaaa cagcgtgcgc tgaatgaccg
      181 aagtcgtaga cgcgatcgcc atggcctcgt cgtagtcgcg aatgcgtttc tgaaacccgg
      241 cgtcacgatc gatgccggct ttgcgccccg cttcagagag cagcagcaga tcgagcagtt
      301 cgctggcgaa tccctgatct gacagtttct cacgatccaa ctcggcagcg tctttcagct
      361 gacgaaaggt cggcaggtcg atgatggttc cgccgacctt cagggcgact tcacgttcgg
      421 cgaaatacag gggaaaatcg attttcatgc acagggtcat ggcgaagccg gtaaacagga
      481 cgagtcccag cgccatgccc cacagagcgg cgcgtgagat ctgcagatac gattcgggaa
      541 caggtgtggt ttcctgggac attatgggcc tcttgcgggt tcggaaggtg aaagaacctt
      601 ttctgatgca cttgcaggcg cggacagact cgtactcgcc ggcaacgttt cctgggtgtc
      661 ggaagcagag gagggcaagg agagtgacag gttgctcgag gccggcgtcg gccccttcat
      721 gagggcaagg atctcctgga gatcgaacgg tccttcgcgt gagtagcgaa tgacaccacc
      781 cggatcaacg aggaagacgg acggagtcgt gcgcactccc cacttatctg cggttagata
      841 tgcggctgag gccacttcgt cgaccagcag agtggcttcg agtcgacgct ttttctgaaa
      901 attctgcagc cccttgcgca tgcgactgcc gtcgagagag atgaaaaaag gcgtacagga
      961 aggggaggcg agatgagcag tttcctcgat caggtgcgga atttcatcga gacaggtatg
     1021 acagaatagc gaccagaaga aaacgattga ccagcttccc gtggccggaa cattgtgttt
     1081 tgtaccggtg agatcttcga tgtttgttgt tggagcgtgg cttccggggg cgagaaatgg
     1141 ttctggtgcc gcggttgcaa cctgggccag cgcgaaaaaa acgaacaaga cgagcagaac
     1201 aaccccatct gaacggaaca acaccgtcgg taaacaagta caatggacga ggttgccggg
     1261 agagtgatca gagtttttca gcaatggcat tctcgatctc cgtttccatg ctttgatcgt
     1321 atccaaggtg ggaatacaga atctgcccgc gacgatccga caagaccact ttggggagga
     1381 tcgtgacctg aaacgctttc atcagggcgg attcgtcatc gatggcaatc tgatagggaa
     1441 tctttgtggg aaacttgcgc agaaacttct ggacctttgg caacgggaac ccgggaggat
     1501 ggatgccgat gatggtgagc cggtctttcc agttttcgtg cagatgaacg atgaagggaa
     1561 tctcctcgat acaggtggcg caggtgatag accagaagac gaagagaatt ttcttgtcag
     1621 aaaacagccc cgaaagcttg aatttttcgc cattcacggt agacagggtg acgccctcga
     1681 gagagccaag gcttgagggt ggggtgtccg gccccagcaa ccagagaagg ggcagggtaa
     1741 gaat
//
LOCUS       DAORHD010001159         1744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_89977_length_1744_cov_2.239787, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001159 DAORHD010000000
VERSION     DAORHD010001159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1744
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89977_length_1744_cov_2.239787"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="PKO06_19645"
     CDS             complement(<1..1154)
                     /locus_tag="PKO06_19645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV71928.1"
                     /translation="MFQNGQIIGRRFRILRPLGAGGMGHVYKAHDENLGREVAIKLLA
                     DHAVQDQTVIDRFFHEGRVLAAIKHPAVIDIYSFETDEQLGIPFFVMEYVDGSLFSEL
                     KPTLLHDRTRLASYLLQLCEGIQACHERGVIHRDLKPTNILVDQRGRLKLFDFGIAKT
                     TLQQTQVGQTLGTPYYMSPEQCMGSRELSPKSDMYSLGVIFWEFLFGQVPFDAAPHAG
                     AEVAIAVQHIQATLPQVAIDPHDPLLPLVPLVRRMLDKDPASRPEVAEIITALQGFLG
                     TKAGVIRNASVSVPAAQASGSTLSTGEKLAASAFALFVLFTMCFGLYDRYNPSADTPA
                     ETLTASGTPPLSLSQTASDATTTAIAPPLASSALVSTTASATVPITVTETP"
     gene            1280..>1744
                     /locus_tag="PKO06_19650"
     CDS             1280..>1744
                     /locus_tag="PKO06_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71929.1"
                     /translation="MDELQQRQLESLKREIDRRHEEHRAGASGKRRWRRLFRLFLLGA
                     AALFLVLAVVFFVNFLRLNQELVEVQIGQRLLPRLLGGKAAFSFARRSGNILSKPVLE
                     QVVIRNPAFSSGGALLFIGRAELSYSLLDVFWGKATLEKILLDDVVVTLSHNE"
BASE COUNT          360 a          467 c          554 g          363 t
ORIGIN      
        1 ggagtctcgg tcaccgttat cggaacagta gcggatgccg ttgtggacac gagagcggag
       61 ctggccaacg gtggagcgat tgccgttgtg gtcgcatcag aagccgtttg cgaaagggag
      121 agtggtggcg ttccggatgc ggtcagagtt tctgcaggcg tgtcggcaga ggggttgtag
      181 cgatcgtaca agccgaagca catcgtaaac agcacaaaca gagcgaaagc gctggctgcg
      241 agtttctctc cggtcgagag ggtggaccct gacgcttgag ccgctgggac tgaaacagag
      301 gcgtttcgga tgacacccgc cttcgtcccg agaaagcctt ggagcgccgt gatgatctct
      361 gccacttccg ggcgacttgc cgggtccttg tcgagcatgc gacgaaccag tggaaccaaa
      421 ggcagaagcg ggtcatgcgg atcgatggcc acctgaggaa gagtggcctg gatatgctga
      481 accgcgatgg ccacttctgc accagcgtgg ggtgcggcat cgaatggtac ctggccgaac
      541 aggaactccc agaaaatgac gcccaacgaa tacatgtccg atttggggct caactctcgg
      601 gatcccatgc attgttcggg cgacatgtag tacggcgttc cgagggtctg cccgacctgg
      661 gtttgctgca gggtggtctt ggcgatgccg aaatcgaaga gcttgagccg accgcgctga
      721 tcgaccagaa tgttggtcgg tttcaggtct cggtgaatga ccccccgttc atggcaggcc
      781 tgaatgcctt cgcacagctg cagcagatac gaggccagac gggtgcggtc atgcaacagg
      841 gtgggcttta gctctgagaa caatgagccg tccacgtact ccatgacaaa gaatgggatg
      901 ccaagctgct cgtctgtctc gaacgaataa atatcaatga cggcagggtg cttgatcgcg
      961 gcgagaacgc gcccttcgtg aaaaaagcgg tcgatgaccg tctggtcctg caccgcgtga
     1021 tccgccagaa gcttgatcgc cacctcgcgt ccgaggttct cgtcgtgcgc tttgtacacg
     1081 tgacccatgc cgccagcgcc cagtggacgc aggatgcgaa agcggcgacc gatgatctgg
     1141 ccgttctgaa acatggcggg tactgtacgc ccggccgacg cgttcgtcaa gaaaaccgga
     1201 acgacacgcg caggcgagat tcaaacctga ccggtttccg gtgttggggg gaccagtcga
     1261 cttatgctac agtacaagaa tggatgagct gcaacagcgg caactcgagt cgctgaaacg
     1321 tgagatcgat cgacgccatg aggaacatcg ggcgggtgct tccgggaaac gtcgctggcg
     1381 aagactgttc cgtctgtttc ttctgggagc ggcggctctc ttccttgtgc tggcggtcgt
     1441 ctttttcgtc aactttttgc gcctgaatca ggaactcgtg gaggtgcaga tcggacaacg
     1501 tctgctccca cggctgctcg gcggaaaagc ggccttcagt ttcgcacggc gttcaggcaa
     1561 catcctcagt aagccggttc tcgagcaggt ggttatccgc aaccccgcat tctcgagcgg
     1621 cggtgccctg ttgttcatcg gtcgggccga actgtcctat tcgcttctcg atgttttctg
     1681 gggcaaagcg actctcgaga agattctgct cgatgatgtt gtcgtgacct tgagtcacaa
     1741 tgag
//
LOCUS       DAORHD010001160         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90092_length_1742_cov_4.071132, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001160 DAORHD010000000
VERSION     DAORHD010001160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90092_length_1742_cov_4.071132"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1742
                     /locus_tag="PKO06_19655"
     CDS             <1..>1742
                     /locus_tag="PKO06_19655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV71930.1"
                     /translation="QIHATLLETRGRTLATTLASGVPDALLQLADVPATQSIQIIPFE
                     TPQGDFFIALRRKLDLPSPTHLMLLLTTTATDRHLQHLLPFFLFIVVSFLLLAALLSA
                     QIAASISSQVDELTATLIDSEQRQRTRAEQLEKAIAHFQKLLEAADQGDSLSVRFENP
                     HLATCWEVKQCRHTDCPAHGRVAQRCWQIVGRYGCLSATGQQKACNDCEIFRHCTRDQ
                     ILQIGEIFNNLMARLQKKHQSEAEALERSQRLAFQAENANRAKSAFLATMSHEMRTPL
                     SGITGTADLLCATPLTTEQRLYAETVRASADHLLSVINDVLDISRIEAGKLELEIQNF
                     HLLDVVEETCSVLASQLQDKPVILSFYPCNTLPEMVAGDHKRFRQVLMNLVGNAIKFT
                     ATGSIDLQAEYVQESELHYLIRFSVRDTGSGISPEQIPRLFNLYEQGDSSFSRRYGGT
                     GLGLAISKRLIQMMGGDVKVESVPGAGSLFTFTLLFQKAAERPVPLAVPANLRDRSVL
                     AILRSRSVERMLDWQLAACGLCVEHGWTEQASSPSRVACLLVEIPPNDQDIWTMLEAI
                     RQDQVLGSLPLIGV"
BASE COUNT          349 a          574 c          460 g          359 t
ORIGIN      
        1 cccagatcca tgccacactg ctcgaaacgc gcggtcgcac gctggccacg acgctcgcgt
       61 caggggtgcc cgatgcattg ctgcagttgg cagatgtacc agccactcaa agcattcaga
      121 tcattccttt cgaaactccg cagggggact ttttcatcgc tctgcgccga aaactcgatc
      181 ttccctcgcc cactcatctg atgcttctgt tgacgacaac cgcaaccgat cgtcaccttc
      241 agcatctgct ccccttcttc cttttcatcg tcgtctcgtt tctgctgctc gccgccctac
      301 tttccgcgca gattgccgcc agtatcagct cacaggtcga cgaactgacc gccacgctca
      361 tcgactcgga acagcgccag cggacgcgcg ccgagcaact cgaaaaagcg attgctcatt
      421 tccagaaact gcttgaggcg gcggatcagg gagactctct ctccgtccgt ttcgaaaacc
      481 cgcatctggc gacctgctgg gaagtcaagc agtgccgaca taccgattgc ccggcgcacg
      541 gtcgcgtcgc gcagcgctgc tggcagatcg tcggtcgcta tgggtgtctt tcagcgactg
      601 gacagcagaa ggcctgcaac gactgtgaga tctttcgcca ctgcacccgc gaccagattc
      661 tgcagatcgg agagattttc aacaacctga tggcccgcct gcagaagaaa catcagtccg
      721 aggcagaagc gctggaacgg tctcagcgac tggcttttca ggcggagaat gccaatcggg
      781 ccaagagcgc gtttctggcg accatgtcgc atgagatgcg cacccctctc agcgggatca
      841 ccggaaccgc cgatctgctg tgcgccaccc ccctcacgac ggaacagcgc ctctatgctg
      901 aaaccgttcg tgcatcagcc gaccatctgc tttccgtcat caatgacgtg ctcgacatct
      961 cacgcatcga agccggcaaa ctggaactcg agatacaaaa cttccatctg ctcgatgtcg
     1021 tcgaggaaac ctgttcggtg ctcgcgtccc aactccagga caaaccggta atcctctcgt
     1081 tctatccctg caatacgctc cccgagatgg ttgccggaga ccacaaacgt ttccggcagg
     1141 tcctcatgaa tctggtcggc aatgccatca aattcacggc cacgggcagt atcgacctcc
     1201 aggccgagta tgtgcaggaa agcgagcttc actatctcat tcgtttcagc gtacgcgaca
     1261 ccggctcagg aatctccccc gagcagattc cccgactctt caacttgtac gaacaaggtg
     1321 actcgagttt ttcgcgtcgt tatggcggca ccggtctcgg gctggccatc tcgaagcgac
     1381 tgattcagat gatgggagga gacgtgaaag tagagagcgt gccgggagcc ggcagcctct
     1441 tcaccttcac gctcctgttc caaaaagcgg ctgagcgccc cgtgccgctt gccgttccgg
     1501 cgaatttgcg cgatcgctcc gtgctggcga tcctacgatc tcgctcggtc gaacgcatgc
     1561 ttgactggca gctggccgcc tgtggcctgt gcgttgagca cggctggacg gagcaagcca
     1621 gctcaccgtc gcgggtcgcc tgtctcctcg tcgagatccc ccccaacgac caggacatct
     1681 ggaccatgct cgaagccatc cgccaggacc aggttctcgg ttcgctgccc ctcatcggcg
     1741 tg
//
LOCUS       DAORHD010001161         1740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90266_length_1740_cov_4.199407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001161 DAORHD010000000
VERSION     DAORHD010001161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90266_length_1740_cov_4.199407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1465)
                     /locus_tag="PKO06_19660"
     CDS             complement(<1..1465)
                     /locus_tag="PKO06_19660"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNV71931.1"
                     /translation="MNPSQQMRDMLETSRRLWGRPSDADLPALEHRLQLLAEREATQT
                     DGLLVGLVGGTNVGKSTLINALAGQEIAPTSARASYSLRPAVYVHRNREAMARTRLAG
                     LLQPEDRFLVHAVEPLERLILVDSPDFDGINEENRDVFRQVLARLDLALCVVTTQKYD
                     SLKLYEILGREMGYRRIAFVFNRIDEGFPFTPVIREDLLSKIAPFSLRPPQGDTLPVF
                     AISALNYYLQRCGQTAGPAGDVPALLSLLQERLSARVVREINEEHFRLALEETRAALW
                     KAGEWEKARRTIENMLQMAIEGLTHLYNSLKTSAVESQALLDSEYRFRHASRLASGFD
                     GPFGGYLRLWMAFRGLLEGLPAGESSFLPACQDFLRRAEPTLLSESLLISRRLADEAD
                     RCGFLAQAQRRIPLAEKTLPPHPGDLAVFLRQQMAEQKIAWWENLALNLIPTTIILLL
                     ARYFVVCLVEVRDPAAGMFLGGFLIFWLVCQFQAIFWY"
     gene            complement(1462..>1740)
                     /locus_tag="PKO06_19665"
     CDS             complement(1462..>1740)
                     /locus_tag="PKO06_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71932.1"
                     /translation="IAIPVFHALERALPERVLELIDRLSREPFLTNTRDRFIQTRREI
                     FAETGRLIAEPVHQLFTGPEVTALDIHTQLDHLQNLWQSYSREVVKTP"
BASE COUNT          387 a          479 c          541 g          333 t
ORIGIN      
        1 cataccagaa gatggcttga aactggcaga cgagccagaa gatcagaaaa ccgccgagaa
       61 acatgcccgc ggccgggtcg cgtacttcca cgaggcagac gacaaaatag cgggccagaa
      121 gcagaatgat cgttgtcggg atcagattca gagccaggtt ttcccaccag gcaattttct
      181 gctcggccat ctgctggcgc agaaacacgg cgagatcgcc cggatgtggc ggcagcgttt
      241 tctcggcgag tggtatccgt cgttgcgctt gcgcgagaaa gccgcatcga tcagcctcgt
      301 cagccaatcg tcgcgagatc aagagagact cagaaagcag ggttggttcc gctcgtctga
      361 gaaagtcctg gcaggctggc agaaaagaac tctctccggc cggtagacct tcgagcagtc
      421 cgcgaaaagc catccagagg cgaagatacc cgccaaatgg gccgtcgaag cccgaagcga
      481 gacgcgacgc atgcctgaag cgatactcgg aatcgagcaa tgcctgtgat tcgaccgccg
      541 aagtcttgag ggaattgtac aagtgcgtta ggccttcgat ggccatttgt agcatattct
      601 cgatcgtgcg ccgcgctttt tcccattctc cggctttcca gagcgcggcg cgggtttctt
      661 ccagggccag gcgaaagtgc tcttcattga tctcgcgcac cacccgcgca ctcagccgtt
      721 cctgcaagag tgaaagcaga gcgggaacat cgccggccgg ccccgcggtc tggccgcagc
      781 gctgcagata gtagttcagg gcagagatgg cgaagacggg gagcgtgtct ccctggggtg
      841 gacgcagtga gaacggcgca atcttactca acagatcttc gcgaatgacc ggagtgaacg
      901 ggaacccttc gtcgattcga ttgaagacga aagcgatgcg ccgatagccc atctcgcgtc
      961 cgaggatctc gtacaatttc aaagaatcat acttctgcgt ggtgacgacg cagagagcga
     1021 gatcgagtcg cgccagaacc tgacgaaaga cgtcgcgatt ttcctcgtta atgccatcga
     1081 aatcggggct gtctaccaga atcagtcgtt cgagaggttc gaccgcatgc accagaaaac
     1141 gatcttcggg ttgcaacaac ccggccagac gggtgcgcgc catcgcctcg cgatttcgat
     1201 gcacatagac cgcgggtcgc agtgaataag aagcgcgtgc cgaggtcggg gcgatttcct
     1261 gcccggcaag tgcgttgatg agcgtcgact tcccgacatt ggtgccgccg accagaccca
     1321 ccagcagtcc gtccgtctgc gtggcttcgc gttcggcgag caactgcagg cgatgctcga
     1381 gggcgggtag atcggcatcg ctcgggcgac cccagagtcg gcgggaggtt tcgagcatgt
     1441 cacgcatctg ttgcgaggga ttcatggggt tttgacgacc tcccgggaat agctttgcca
     1501 gaggttctgc agatgatcaa gttgggtgtg aatatcgaga gcggttactt ccgggcccgt
     1561 gaacagctgg tgaacgggct cggcaatcaa cctgcccgtt tcggcaaaaa tctcgcggcg
     1621 ggtctggata aaccggtctc gtgtgttggt gagaaatggt tcacggctca gacgatcgat
     1681 gagttccagc acccgctctg gcaaggcgcg ttcgagcgca tgaaagacgg gaatggcgat
//
LOCUS       DAORHD010001162         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90626_length_1736_cov_3.498513, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001162 DAORHD010000000
VERSION     DAORHD010001162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90626_length_1736_cov_3.498513"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="PKO06_19670"
     CDS             <1..557
                     /locus_tag="PKO06_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71933.1"
                     /translation="NLPADWSHTHTFEMMPEWGTNPLHRTWIIRLPTHLEEKDRYLFH
                     YYFQVHYHNGTERVSHTFTQLIMPRPFEFLDHAGDFLYVRLHWSVGAWTYPQDTEMEV
                     EGIDWGSEFSVSNVPYRGTDRLFHNGRVLVMKRLVPPRRFRGYIWAPKGSEIRYCFQM
                     ARYKGDGFDIVWDNNFGRDYILTI"
     gene            595..1161
                     /locus_tag="PKO06_19675"
     CDS             595..1161
                     /locus_tag="PKO06_19675"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNV71934.1"
                     /translation="MRHPDILTREGAVLLICDVQKKCIKPIYRKEPMINNIKTLVSFA
                     GMVGLPILLTELAPQNFGGTIDEIKKLVPRLDPIKRSRYTCFGNEDLTLRLEAMNTKT
                     LVVAGMESHISVCQTVLDALTRGFRVHVVSDATSSRRRVDRRVALEKMRRAGAVVTTM
                     EMVMHEMVERVDVPQFEKFQELVGNIGR"
     gene            1164..>1736
                     /locus_tag="PKO06_19680"
     CDS             1164..>1736
                     /locus_tag="PKO06_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71935.1"
                     /translation="MNDILAPTPTLTFLIGGLGAGKTELALNLALSYAEVHGSGRVHL
                     VDLDVVNPYFRVRKVREELSRSGVHVVTPDAKVREGDLPALPAAAWGAFEAPQEWTVV
                     DIGGGELGLRPLGRIREIARCRPGSVYFVVNPYRPGYRTLEEMEKNFRHLQALSALTV
                     THLVANPHLVHETTGDLFQRGLTQVQSLSER"
BASE COUNT          363 a          484 c          514 g          375 t
ORIGIN      
        1 cgaatctgcc ggcggactgg tcacacacgc acaccttcga gatgatgccc gaatggggaa
       61 ccaatccact gcatcgcacc tggatcatcc gcctgccgac gcatctcgaa gaaaaagacc
      121 gctacctgtt tcactactat tttcaggtgc attaccacaa cggtaccgag cgtgtcagtc
      181 atacgttcac gcagttgatc atgccacgcc ccttcgagtt tctcgatcac gcgggcgatt
      241 tcctgtatgt ccgactgcac tggagcgtcg gggcatggac atacccgcag gataccgaaa
      301 tggaagtcga aggcatcgac tggggcagtg agttctcggt cagcaatgtt ccctaccgcg
      361 ggaccgatcg tctgtttcac aacggtcggg ttctggtgat gaagcgcctg gttccgcccc
      421 gtcgctttcg cggctacatc tgggcgccca aaggctctga aatccgctac tgtttccaga
      481 tggcccggta caagggagac ggctttgaca tcgtctggga caacaacttc ggacgcgact
      541 acattttgac tatttgattt ttgtacaaac caccggcgag gagataaccg tatcatgaga
      601 caccctgaca ttcttacccg cgaaggtgcc gttctgctga tctgcgacgt gcagaagaag
      661 tgcatcaagc cgatttatcg caaagagccg atgatcaaca acatcaagac tctggtgtcc
      721 ttcgccggca tggttgggtt gcccatcctg ctgaccgagc tggcaccgca gaacttcggc
      781 gggacgatcg atgagatcaa gaagctggtt cccaggctcg atcccatcaa acgaagccgg
      841 tacacctgct ttggcaacga agacctgacg ctgcgcctcg aagcgatgaa taccaagaca
      901 ctcgtggtgg ctggtatgga aagccacatc tcggtgtgtc agacggtact cgatgccctg
      961 acccgcgggt tccgggtgca tgtggtttct gacgccacga gctcgcgccg ccgtgtcgat
     1021 cgccgcgtgg ctctggagaa gatgcgccgc gccggagcgg tcgtgacaac catggagatg
     1081 gtcatgcacg agatggtcga gcgggtcgac gtgccccagt ttgagaaatt ccaggaactg
     1141 gttgggaaca tcggtcgctg accatgaacg acattcttgc cccgaccccg acactgacct
     1201 ttctgatcgg cgggctggga gcgggaaaga cagagttggc gctcaacctc gccctgagtt
     1261 atgccgaagt gcatggctca gggcgagttc atctcgtcga tctcgatgtc gtcaacccgt
     1321 actttcgtgt tcgtaaagtg cgagaagaac tttctcgcag cggagttcac gtggtgactc
     1381 ctgatgccaa agtacgggaa ggtgacctgc cggcgttgcc ggcggctgcc tggggggcat
     1441 ttgaggcgcc gcaggagtgg acggtggtcg atatcggcgg tggcgagctg ggattacgtc
     1501 cgctgggccg catccgggaa atcgcccgct gtcgtcccgg cagtgtctat ttcgtggtca
     1561 atccgtatcg tccggggtat cgcacactgg aagagatgga gaagaatttt cgacatctgc
     1621 aggcgctatc ggcattaacg gtcacacatc tcgtggcgaa tccacatttg gtgcatgaaa
     1681 cgaccggtga tctgtttcag cgtggtctga ctcaggtgca gtcgctcagt gagcgc
//
LOCUS       DAORHD010001163         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90724_length_1735_cov_3.488690, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001163 DAORHD010000000
VERSION     DAORHD010001163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90724_length_1735_cov_3.488690"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PKO06_19685"
     CDS             <1..756
                     /locus_tag="PKO06_19685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HNV71936.1"
                     /translation="LKLILQELRTGQHQVDSLRCLVSSSALLDPSLKAELLEHLPCDF
                     HECYGTSEIAIASNLDRAAGLRKTASVGVAAPGVDIRILDDQQKPLPANQPGEIACRT
                     PMRFAGYYQRPDLTDAAMWGEYFRTGDIGKLDDEGFLFFLGRKKEIIITGGVNVYPQD
                     VESALLTHPQVSECAVVPMPDNRLGEVVCAVIVARDRSQLNIRDLQRISARQLADYQQ
                     PHHYIILDELPKNRMGKLMKQELLKLLQERKYQ"
     gene            841..1704
                     /locus_tag="PKO06_19690"
     CDS             841..1704
                     /locus_tag="PKO06_19690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNV71937.1"
                     /translation="MTAKPRAIYGASLFGRVLFEELQKLGEEIDFFIDEYTPQATYLG
                     KPVRRLKEVDDKDQITVMNSTAYPLEQQLREKGFGQVLSLREIVNTYPQTLKNLNETM
                     WALKSHRLVDEAEFAWLAQRLQDNDSKALLETITKFRQNPVPENLYFSDGDPFYFPSG
                     FDSFRGIDRLRFVDCGAFTGDTVQSVFNRFPRAIDWIAALEPDPHNLEKLRNQRFNRP
                     NPHHANADVFVFPVGVFDREQLCHFQMTSEGSSSAITSDATGFSIPVARLDVLFAHAA
                     PNYIKMDVEGV"
BASE COUNT          404 a          502 c          442 g          387 t
ORIGIN      
        1 ctgaaactga ttttgcagga actccgaacc ggtcagcatc aggtcgacag tctgcgctgc
       61 ctggtctcat cttctgccct gctcgacccc agtctcaagg cagaactgct cgaacatctt
      121 ccctgcgact ttcacgagtg ctatggcacc tcggagattg ccatcgccag caatctcgat
      181 cgcgccgcgg gcctgcgcaa gaccgcctcc gtcggggtcg ccgcacccgg agtcgatatt
      241 cgcatactcg atgaccagca gaagccgctg ccggccaatc agcccggcga aattgcctgc
      301 cgcaccccca tgagatttgc cggctactat cagcgccctg atctcaccga cgccgccatg
      361 tggggcgagt atttccgcac cggcgacatc ggcaaactcg acgatgaagg cttcctgttc
      421 tttcttggtc ggaaaaagga gatcatcatt actggtggcg tcaacgtcta tccgcaggac
      481 gtcgaaagcg ctctgctcac tcatccgcaa gtgtccgagt gcgcagtcgt gccaatgccc
      541 gacaatcgcc tgggagaggt cgtctgcgcc gtcatcgtgg cccgtgatcg ttcccaactg
      601 aatattcgtg atctgcagcg catttcggca cgacaacttg cggactacca gcaacctcat
      661 cactacatca ttctggacga gctacccaag aaccgcatgg ggaaactgat gaagcaggaa
      721 ctcttgaagc tgttgcaaga acgaaagtat caatgagcga agagtgcgac caattaccga
      781 ttcgcggagt ccgagtcaag tctggctgat gtaaatgtca cccctgagga gaattgtcac
      841 atgacagcaa aaccacgtgc gatctacgga gcaagtctgt ttggtcgggt gttgttcgag
      901 gaactgcaga agcttggcga agagatagac tttttcatcg atgagtatac gccgcaagca
      961 acctatctcg gcaaacccgt gcgccgtcta aaagaagtcg acgacaaaga ccagataacc
     1021 gtcatgaatt ccaccgcgta tcctctggaa cagcaattgc gagaaaaggg gttcggacaa
     1081 gtcctcagcc ttcgggagat cgtgaacacc tatccacaga ccctgaaaaa tctgaatgaa
     1141 accatgtggg cactcaagtc acaccgcctg gtggacgagg cagagtttgc ctggttggca
     1201 cagcgactgc aggacaatga cagcaaagct ctgctggaga caatcacgaa gtttcgtcaa
     1261 aaccccgtgc ctgagaacct ctatttttcc gacggtgatc cattttactt tccgtcgggc
     1321 tttgactcat ttcgcggtat cgaccgcctg cgatttgtcg actgcggggc ttttaccgga
     1381 gacaccgtac aatccgtgtt caaccgattc ccgcgtgcca tagattggat cgccgctctg
     1441 gaaccggatc ctcataatct cgagaaactg cggaaccagc gtttcaaccg tcctaacccg
     1501 catcatgcaa atgccgacgt ctttgttttt ccggtcggcg tttttgatcg cgaacaactc
     1561 tgtcactttc agatgacttc cgaaggctcg agttcggcga ttaccagcga tgccaccggt
     1621 ttttcgattc ccgtcgcccg tctcgacgtg ctctttgctc atgccgctcc aaattacatc
     1681 aaaatggatg ttgagggtgt ctaacctgaa gcattggtgg gcgcccgaga gatta
//
LOCUS       DAORHD010001164         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90801_length_1734_cov_3.413341, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001164 DAORHD010000000
VERSION     DAORHD010001164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90801_length_1734_cov_3.413341"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PKO06_19695"
     CDS             <1..1351
                     /locus_tag="PKO06_19695"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV71938.1"
                     /translation="PLDGLEEEPLPRGRFARLSPPPEITVTRLGNGLAIVLHPFPSPS
                     IGLSFLVRCGPVFEEAHNNGISHFLEHMLFRGTPEYPSGSQLNMALDRVGAEANAATF
                     SDMTVVSMRLLPEALSDGLELFRRMLSVPVCEGITAERRIIIEECLEDFDEEGRLIAI
                     DQLSSQLLFGKHPYSLPITGEPKVLKRLTRHDLHEHLQRHYRPDAGVICLSGAIETAT
                     VMDAVRERFGNWRNPAGPARLSSTLPEPIFAGPQLVRVDSPRSQVNARISFRTVSFAD
                     PDYYLLKALIRILDAPAGSPLRQKVQDENGFCYNFSAGLDAYERAGAVHIDMTVQPDR
                     LYDALEVSLNVLADLREQGVPPEIVEHMIAQYIKGKRFAATDHWDFCSRYAFRELFPS
                     PHDFATEFHLTQKIVSADLQRLIHQVFRRRHLGLTLVGPISRALAKRIESLLAAFPE"
     gene            complement(1426..1623)
                     /locus_tag="PKO06_19700"
     CDS             complement(1426..1623)
                     /locus_tag="PKO06_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71939.1"
                     /translation="MVDRVSGARPSSVQPAESRAPEGPRGADSSQNAVDLEKTRRASQ
                     AQTRTVKAQDQNLGTIIDIKA"
BASE COUNT          331 a          523 c          477 g          403 t
ORIGIN      
        1 ccctctcgac ggactcgagg aggaacctct gcctcggggc cggttcgctc gcctttcccc
       61 gccccccgag attacggtca cccgcctcgg gaacggtctc gccatcgttc tgcacccatt
      121 tccgtcgccc tcgatcggct tgtcgtttct ggtgcgatgc ggcccggtct ttgaagaggc
      181 gcataacaac ggcatctcgc attttctcga gcacatgctg tttcgcggca caccagaata
      241 cccctcgggg tcacagctga acatggcact cgatcgtgtg ggcgctgaag ccaacgcggc
      301 taccttttcc gacatgaccg tcgtttccat gagactgctt ccggaagccc tttccgatgg
      361 gctggagttg tttcgccgca tgctgtccgt ccccgtctgt gagggaatca ccgccgagcg
      421 acgcattatc attgaggaat gccttgagga cttcgatgaa gagggaaggc tgatcgccat
      481 cgatcaactg tcatcgcagc tgctgttcgg caagcacccg tattctctgc cgatcaccgg
      541 cgagccgaaa gtgctgaaac ggctgactcg ccacgacctg catgaacatc tgcaacgtca
      601 ttatcggcct gacgctggcg tcatctgcct ttcaggggca atcgaaacgg caacggtcat
      661 ggatgccgtg cgcgaacgtt ttgggaactg gcgaaacccc gcggggccag cccggttgag
      721 cagcaccctt cccgaaccga tttttgccgg gccacagctc gtacgagttg attcgccgcg
      781 aagtcaggtt aacgctcgca tctcgtttcg tacggtttct ttcgccgacc cagattacta
      841 tctgctgaaa gcattgatca gaattcttga tgcgccggca ggctcgccac tgcgtcagaa
      901 agtgcaagac gagaatggat tctgctacaa cttcagtgcc ggccttgacg cctatgagag
      961 agccggtgcc gtgcacattg atatgaccgt acaacctgat cgactgtatg atgcgcttga
     1021 ggtaagcctg aacgtgcttg ccgatcttcg cgagcagggt gtgccacccg agatcgtcga
     1081 gcacatgatc gcgcagtata tcaagggaaa gcgtttcgcc gccaccgatc actgggactt
     1141 ctgcagccgt tacgccttcc gcgagctttt tccctcgccc catgatttcg cgaccgaatt
     1201 tcacctgacg cagaaaatag tttcagcaga tctgcaacga ttgattcatc aggtttttcg
     1261 acgtcgtcac ctcggactca cactcgtcgg cccgatttca agggcactcg caaagcgaat
     1321 cgaatcgctc ctggcggcgt ttcccgagta gcgtttcggc caacacgaat aaaaaaaagc
     1381 gcccgcgatt tcgggggcac ttttcttact ccgcgtgtga cagggtcagg ctttgatatc
     1441 gatgatggtt ccgaggttct gatcctgggc tttgacggtt ctggtctggg cctgcgacgc
     1501 acggcgggtt ttctcgagat cgaccgcgtt ctggctggaa tcggctcccc tggggccttc
     1561 tggggcgcgc gactccgcgg gctgaacact cgacgggcgg gcaccggaca ctcgatcaac
     1621 cataccgcac ctccatgagg gtcttcttta aatatacggt ttccgtatcg aaaggcaacg
     1681 ccattccacg ttctatgggc gctgttcttc ctttgcctgt gaagttgccg ggct
//
LOCUS       DAORHD010001165         1734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90807_length_1734_cov_3.120310, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001165 DAORHD010000000
VERSION     DAORHD010001165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90807_length_1734_cov_3.120310"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1734
                     /locus_tag="PKO06_19705"
     CDS             <1..>1734
                     /locus_tag="PKO06_19705"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNV71940.1"
                     /translation="VLMGLFVAIAEEEGMLFLIFSLLFLMLAPFGALRLSGYLQSQMS
                     MQTISTDEEVPILTPAPSVPFESIREWLVKQAWLIGLFLGLIPLATTTNAGYELCLLP
                     SLLIAARLLYMLISTFVNQYGEQAPNSVAAIRRLSTIALTFLPPLLWLRSLGSFPEFL
                     FLGEFTLKILLVLPAYALFRHREELLSTINRVLPTIPSSTDQLLGWVYRACTALLVPG
                     LLLAIYGYQNLALWFLLKLCAIIGVLALLSIGRPVADRLAEHWFLVRPESGSGEAPIS
                     FLHILTRKLLRFLIWVIALAALIKIFGLSRDFPLLSPLTTWIAANGSWVSGFLVKLGL
                     ILGMVVLSLDFCATIGESILAYVRQTEHLPNTESERRASTLVQIFRTTTRVLIFGIAG
                     IMVLQEIGVDIKPLLTGAGILGVAIGFGSQSLIKDFFSGFFILVENQFRVGDVIDVNG
                     KAGVVEKINLKTTVLRAVDGSIFIIPNGEITSVKNMTFAWSRAVLEIGVSYDADLDKT
                     MEVLQRIGDELRFHPSTKGNIIERPEVTGVERLEDSSVVLRLLVKTKPLSQWEIARVF
                     RKRILEVFRQE"
BASE COUNT          313 a          525 c          481 g          415 t
ORIGIN      
        1 cggtgctcat gggattgttc gtcgccatcg ccgaagaaga aggaatgctg tttctcatct
       61 tcagcctgct ctttctcatg ctggccccgt tcggagccct gcggttgtcc gggtatctgc
      121 agtcacagat gtcgatgcag acgatatcga ccgatgaaga agttccgatt ctcacccctg
      181 caccatcggt gcccttcgag tcaatacgcg agtggctggt caagcaggcc tggctgatcg
      241 gtctgtttct gggactcatt ccacttgcga cgacaacgaa cgcaggctat gagctttgcc
      301 tgctgccgag ccttctcatt gccgcccgac tgctttacat gctcatttct acctttgtga
      361 accagtacgg tgaacaggcc ccgaattcag ttgccgccat tcgacgtctg tcgacgatcg
      421 cactcacgtt tcttcccccg ctgctctggc tcaggtctct gggcagcttt cccgagttcc
      481 tgtttctggg cgaattcacc ctcaagatac tgctggtgct gccggcatat gcgctgttcc
      541 gacatcgcga agagctgctt tccaccatca atcgcgtgct gccgacgatt ccgtcgtcga
      601 ccgatcagct gctgggatgg gtatatcggg cgtgtaccgc gctgctcgtg cccggcctgc
      661 tgctggctat ctacggctat cagaatctgg ccctgtggtt tctgctgaaa ctgtgcgcca
      721 tcatcggagt tctggctctc ctttccatcg gacgtccggt cgccgaccgt ctggccgagc
      781 actggttcct ggtgcgcccg gagagcggct cgggtgaagc accaatctcg tttctgcaca
      841 ttctgacgcg caagctgctg cgctttctca tctgggtcat cgccctcgcc gccctgatca
      901 agatcttcgg cctttcccgt gactttccgc tgctgtcgcc gctcaccacc tggatcgccg
      961 caaacggttc ctgggtttcg ggttttctgg tcaaactggg tctgattctc ggcatggtcg
     1021 tgctgtcact cgacttctgc gccaccatcg gcgaaagcat tctcgcgtat gtacgccaga
     1081 ccgaacatct gccgaacacc gaaagcgagc gacgggcgtc aaccctggtc cagatcttcc
     1141 gcaccacgac tcgcgttctg atcttcggga tcgccggaat catggtgctg caggagatcg
     1201 gtgtcgacat caagccgctg ctgaccggtg ccggaattct cggcgtcgcg atcggcttcg
     1261 ggagccaaag tctgatcaaa gacttcttct cgggcttttt cattctggtg gaaaaccagt
     1321 tccgggtcgg ggatgtgatc gatgtcaacg gcaaagccgg cgtggtcgag aagatcaatc
     1381 tgaagacgac ggttctcaga gccgtcgacg ggagtatctt catcatcccc aatggagaga
     1441 tcacctcggt gaagaacatg accttcgcct ggtcacgcgc cgtgctggaa atcggggtca
     1501 gctatgatgc cgatctcgac aagaccatgg aagtgctgca gcggatcggc gatgaactcc
     1561 gttttcaccc gtcgaccaag gggaacatca tcgaacgccc cgaagtgacc ggtgtcgaac
     1621 ggctcgaaga ctcgagcgtg gtgctgcgtc tgctggtcaa gaccaaacct ctgtcacaat
     1681 gggaaatcgc ccgggtcttc cggaagcgca ttctcgaagt ctttcgccag gaag
//
LOCUS       DAORHD010001166         1733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90886_length_1733_cov_3.660310, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001166 DAORHD010000000
VERSION     DAORHD010001166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90886_length_1733_cov_3.660310"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PKO06_19710"
     CDS             complement(<1..206)
                     /locus_tag="PKO06_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71941.1"
                     /translation="MRDVCLVGLCLLFLNSLLLVDVLAAPTTERDPRSSRDAISRTKD
                     RKAKDKKVDKKQDSEETEQDESLFL"
     gene            complement(215..667)
                     /locus_tag="PKO06_19715"
     CDS             complement(215..667)
                     /locus_tag="PKO06_19715"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV71942.1"
                     /translation="MQLAELCKENMVFLDFAAENKDDALNRMVKAVEGAGGVDDINGL
                     RQALIEREKLGTTGVGGGVAIPHARSATIKDLSIAFFRSKAGIQFNAIDNKPVNLVFL
                     LLAPLSAGGPYLKLLAKISRLLRNDDFRSSLQDAGSAADVIKTLKEHD"
     gene            complement(762..>1733)
                     /locus_tag="PKO06_19720"
     CDS             complement(762..>1733)
                     /locus_tag="PKO06_19720"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV71943.1"
                     /translation="SDLAVLKAELPQGTANIPLGNSDSLRVGEWVVAVGNPFGLGITV
                     TTGVVSALGRELSVDRERTFRNLIQTDASINPGNSGGALVNTRGELIGINTAILPYGQ
                     GIGFAIPVSSARRIIDDLMQFGAVRKTYVGLAVQEINEQLAEYLGIPREGILITDVAS
                     GSPSDKAGLVPGDVLLEIDGKPMRNTGDFKERIDRHGVGDTVRLKISRKGKTGECVLV
                     LQEKNAMRGVAGNRLGIHVSAITPALQQEFRLETDFGVVITGVSRGSLAERLGLEPGD
                     VIQSVNQFLIRSAEDFQTLMEKLGRQRRVGMTVVRGALSQMLMFSLP"
BASE COUNT          383 a          525 c          455 g          370 t
ORIGIN      
        1 agaaagagag actcgtcctg ttccgtctcc tcggaatcct gcttcttgtc gaccttcttg
       61 tcctttgcct tgcgatcttt ggtgcgcgaa atggcatcgc gtgaacttcg cggatcgcgc
      121 tcggtggtcg gtgcggcgag gacgtcaacc agcagcagtg aattcaggaa cagcaggcat
      181 aagcccacca gacatacgtc acgcatgacg tgcctcagtc gtgttccttc aacgttttaa
      241 tcacatcagc cgcgcttccg gcatcctgca gcgaggaacg aaaatcgtcg ttgcgcagca
      301 gacgcgaaat cttggccaac aacttcagat acgggccacc agccgaaagc ggtgccaata
      361 acaggaacac gaggttcacc ggtttgttgt cgatggcgtt gaactgaatg cctgctttgg
      421 agcgaaaaaa ggcaatgctc aagtctttga tggtcgccga acgggcatgc ggaatcgcga
      481 caccgccacc gacgccagtg gtacccagct tttcacgttc gatcaacgcc tgacgcaacc
      541 cgttgatatc gtctactcca cccgcgcctt ccaccgcctt gaccattcgg ttgagggcat
      601 cgtctttgtt ttcagcggcg aaatcaagaa aaaccatgtt ttctttgcag agttctgcga
      661 gttgcatgac tacggcctcc gagaaattgg ggtaccagaa cagagggatt atatcacaag
      721 aatcggcgtt tgcaagaaaa gcgcaacgag gaaatttcgt cttacggaag cgaaaacatc
      781 agcatctgcg agagtgctcc gcgcacgacc gtcatgccca ctcggcgttg tcggccaagt
      841 ttctccatga gggtctgaaa atcctctgcc gatcgtatca agaactgatt caccgactgg
      901 atgacatccc cgggttcgag gccgagacgc tcggcgagag aaccccggct gacccccgtg
      961 atgaccacgc cgaaatcggt ctcaagccga aactcctgtt gcagcgccgg cgtaatggca
     1021 ctgacgtgaa tacccaggcg gtttcccgcc actccgcgca tcgcgttttt ttcctgcagt
     1081 accagcacgc actcgccggt ctttcctttg cgactgattt tcaagcgtac ggtatcaccc
     1141 actccgtgcc ggtcgattcg ttccttgaag tctcccgtat tgcgcatcgg cttgccatcg
     1201 atttccagca ggacatcgcc gggaacgagc ccagccttgt ctgaagggga tccgctagca
     1261 acgtcggtga tcagaatccc ttcgcgcgga atgccgagat actccgccag ttgctcgttg
     1321 atctcctgca cagccagacc aacataggtc tttcgtacgg ctccaaactg catcaggtcg
     1381 tcgatgattc gccgagcgga actgacggga atggcaaagc cgatgccctg cccatacggg
     1441 agaatcgccg tgttgatacc aatcagttcg ccacgcgtgt taaccagcgc ccccccagaa
     1501 ttacccgggt taatcgaggc gtcggtctgt atcagattgc gaaacgtgcg ttctcgatcg
     1561 accgacagct cgcgccccag cgctgaaacc acacccgttg tgaccgtgat gcccaggccg
     1621 aatgggtttc cgacggcaac cacccactca cccacacgaa gagagtccga gttgcccaac
     1681 gggatattcg ccgtcccttg cgggagttcg gccttgagaa ccgccaggtc act
//
LOCUS       DAORHD010001167         1732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_90976_length_1732_cov_4.419201, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001167 DAORHD010000000
VERSION     DAORHD010001167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90976_length_1732_cov_4.419201"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PKO06_19725"
     CDS             complement(<1..606)
                     /locus_tag="PKO06_19725"
                     /inference="COORDINATES: protein motif:HMM:NF025027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HNV71944.1"
                     /translation="MTVSQFQFVAGAWPELFEAASKAESLAISDPRTSCFYARRTLEL
                     AVAWLYKHDAALKLPYQEHLSALIHEPTFRNLVGPAVFAKARILKELGNFAVHNQKPI
                     LEFDAVTAVKELHHVLFWLARTYLRDFQPNAVAAFDPQLLPKTAPIPVQTREELKSLD
                     AKLKERDNHLAMLLADKTKLDAELQRLRTEIVAIKAANASQT"
     gene            complement(603..1172)
                     /locus_tag="PKO06_19730"
     CDS             complement(603..1172)
                     /locus_tag="PKO06_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71945.1"
                     /translation="MRSSVPEKILLIADQIEEDGSVNITKLTIVKKWFAKPQRRKAFA
                     LWMARHAAGKPGKVDANAKRILNDAKKLLGSAPRHGTLSTEIDREKIGALCDRARAFN
                     DEYVPGKWGSVRIIQCQALLLIEKSLAILLVRESLPAEAYELLATWVYSFDSRYSDTL
                     NGPSSGRLREFVRFMFTVEALEHDAEANA"
     gene            complement(1178..>1732)
                     /locus_tag="PKO06_19735"
     CDS             complement(1178..>1732)
                     /locus_tag="PKO06_19735"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNV71946.1"
                     /translation="QLGRQRAPKYQTGKHTHPYVRVANVFEDRIDVSDLLCMDYDQRD
                     FPAYRLEWGDILLNEGQSTELVGRPAMWRNEIADCCFQNTLIRFQANSDIVLPEFALA
                     VFLHYFRTGAFASLSSKTSSVAHLGAGRFSSMPFPLPPLDLQHIFVKRITVVKKTAEA
                     CQLSSAELDALFASLQHRAFRGEL"
BASE COUNT          391 a          451 c          483 g          407 t
ORIGIN      
        1 ggtttgcgag gcgttggcag ccttgatcgc gacaatctcg gtacgcagac gctgaagttc
       61 agcgtccagt ttggttttgt cggccagaag catggccaga tgattgtcgc gctctttgag
      121 ttttgcatcg aggcttttca gctcttcgcg agtttgcacc ggaatcggag cggtcttcgg
      181 caggagctgc ggatcaaagg cggcgactgc gttcggctga aagtcgcgca gatacgtgcg
      241 agccagccag aagagcacat gatgcagttc tttgacggca gtaacggcat cgaattcgag
      301 aatgggcttt tgattgtgaa ccgcgaaatt gcccagttct ttgagaatgc gggctttggc
      361 gaacacagcc gggccgacga gatttctgaa cgtcggctcg tgaatcaggg cgctgagatg
      421 ttcttgatac ggcagcttca gcgccgcgtc gtgtttgtac aaccaggcga cggcaagttc
      481 cagcgttcgg cgcgcgtaga aacacgaggt tcgcgggtca gagatggcca gcgattcagc
      541 ctttgatgcc gcctcgaata gttcgggcca tgcgccggcg acgaactgaa actgagacac
      601 ggtcatgcgt tcgcctctgc atcgtgttca agcgcttcaa cggtgaacat gaatcggaca
      661 aactcgcgca gtcgcccgct actggggccg ttcagcgtgt cggaatagcg tgagtcaaat
      721 gaatataccc aggtcgccag aagttcatac gcctctgccg gcagggattc gcgtaccaat
      781 aatatcgcca ggcttttctc gatcagaagt agggcttggc attgaatgat tcggacggaa
      841 ccccacttgc ccggaacata ctcgtcgttg aaagcacgag cacggtcgca caacgcaccg
      901 attttctccc gatcgatttc ggtagacagc gtgccgtgac gtggcgctga gccgaggagt
      961 ttcttcgcgt cgttgagaat tcgcttggcg ttcgcgtcga cttttccggg tttgcctgcc
     1021 gcgtggcgtg ccatccacaa cgcgaaggct ttgcggcgtt ggggtttcgc gaaccacttt
     1081 ttcacgatcg tcagcttcgt gatgttcacc gaaccgtctt cttcgatctg atcggcaatc
     1141 agcagaatct tctctggtac actgcttcgc atgatgccta caactccccg cgaaacgccc
     1201 gatgctgcaa cgacgcaaac aacgcatcaa gctctgctga ggagagctga caagcctctg
     1261 cagttttctt tacgaccgtg attctcttaa cgaagatgtg ctgtaaatcc agaggtggta
     1321 acggaaatgg catcgaggaa aatctaccgg cacctaaatg cgcaacactc gatgtcttgc
     1381 tgctgaggct ggcgaaggct cctgtgcgaa agtaatgcag aaaaacagca agggcgaact
     1441 caggtaagac gatgtccgaa ttcgcctgaa atcgtatcaa agtattctga aagcaacagt
     1501 cagcaatttc gttgcgccac attgctgggc ggccaacaag ctcggtactt tggccttcat
     1561 tgagcaggat atcaccccat tcaagacgat atgccggaaa gtcccgttgg tcataatcca
     1621 tgcaaagcaa atcagacaca tcgatccgat cttcaaaaac atttgcaact ctgacgtaag
     1681 ggtgagtgtg tttgcctgtt tgatactttg gggctctctg tcttcccagc tg
//
LOCUS       DAORHD010001168         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91134_length_1730_cov_3.717612, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001168 DAORHD010000000
VERSION     DAORHD010001168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91134_length_1730_cov_3.717612"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..>1730
                     /locus_tag="PKO06_19740"
     CDS             335..>1730
                     /locus_tag="PKO06_19740"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71947.1"
                     /translation="MNTRIFVTIALCLGLSVAVCAQDAAAVLYQQGMNLYAQKKYLAA
                     VDYLGQVCDMAPANLQARFYLVYSYIGIGKHPEALKHARVLAEKDPGNMSYQTLKAEL
                     EKHIGVVQAQAAQSVRPPKEVEFGGYETSEPAVKPGEPKEAPKPKPQPPKEPKEKADP
                     MVEKALEALDNQNYEEAARILEEYLGKKPKDFQALHARGMVAVQAAEYEKARTWLEKA
                     LKEKPDAFDTLFLLGEACQRSDKLKEAEEAFAKAVAKKQDQFALINLGEVKKRLGKLT
                     DAVDTLKKALAIDANLVDAKLSLADAYLEQGKTEDAVNLANEVLTSEPGNSTAHFVKG
                     KILYRSDLIDDAITELKAAMIESRENEIIGAYLGRVLLSQQKFAEALDAANEVLKINP
                     NSWEARLILAEALMLSGDNENARDQIVEAEKLYSGPKVMLARARLTRREGDIDKATEM
                     FQAYVTAEGSTAQSL"
BASE COUNT          450 a          448 c          528 g          304 t
ORIGIN      
        1 acccgaagaa agacaagcgc aatacccccg agtgccctag gaacgagagt cgctgatatc
       61 gttcgacgtc gactgcttca cgtcgaccgc ttcacgtcga ccgcttcacg cggtcagagt
      121 tcctgacagt cgcttcgaga gggtatcgtt tccgcaaaga cgaatctgag cgggaagggg
      181 aacggccgaa actctcgaac tgaacatacg cctgagaagc gcgagcggga aagggttcaa
      241 agtttctggt tgcacggctt gatgcacggt tgctgaatcg ggtagaatcc gtcgattgtc
      301 ccggatcaag aggaggcaaa acatgagtag ttcgatgaat acgcgaatct tcgttacgat
      361 cgcgctctgc ctgggtttga gcgtggctgt ctgcgcgcag gatgcagcgg cagtcctgta
      421 tcagcagggt atgaacctgt atgcgcagaa aaagtatctg gcggcggttg actatctggg
      481 gcaggtctgt gacatggcgc cggcgaatct gcaggctcga ttttacctcg tctattccta
      541 catcggcatt ggcaagcacc cggaagccct gaagcatgcc cgcgtgctcg ccgaaaaaga
      601 tccgggcaac atgagctatc agacgctcaa agcagaactc gaaaaacaca tcggggtggt
      661 gcaggcgcaa gcggcccagt cagtgcgtcc gccgaaagag gtcgaattcg gcggctatga
      721 aacgtctgaa ccagccgtga agcccggtga gccaaaagaa gcgccgaagc ccaaaccaca
      781 gccaccgaaa gaaccgaagg aaaaggctga tcccatggtc gagaaggctc tcgaggccct
      841 ggacaatcag aactacgaag aggccgccag aattctggaa gaatatctgg ggaagaaacc
      901 caaagacttt caggcactgc atgcgcgcgg catggtagca gtacaagcgg ctgagtatga
      961 aaaagcgcgt acctggctgg aaaaagccct gaaagaaaaa ccagatgcct ttgatacgct
     1021 gttcctgctg ggagaggcgt gtcagcgctc ggacaaactg aaggaagctg aagaggcctt
     1081 tgccaaggct gtggccaaga agcaggacca gttcgccctg atcaatctcg gcgaagtgaa
     1141 gaaacgtctc gggaagctga ccgatgcggt tgatacgctg aagaaggcgc tggcgatcga
     1201 tgccaatctg gtcgatgcca agctgagtct cgccgatgcg tatctcgaac agggcaagac
     1261 ggaagatgcc gtgaacctgg cgaacgaggt cctcacctca gaaccaggga actcgacggc
     1321 tcacttcgtg aaaggcaaga ttctataccg cagcgatctg atcgatgacg cgatcaccga
     1381 attaaaagcc gccatgatcg agagccgcga gaatgagatc atcggagcgt atctgggccg
     1441 ggtgctgttg agtcagcaga agttcgccga agcgctcgat gccgccaacg aagtgctcaa
     1501 gatcaatccg aacagctggg aggcacggtt gattctggct gaagccctga tgctgtcggg
     1561 tgataacgag aatgcccgcg atcagattgt cgaagccgag aagctctaca gcggaccgaa
     1621 ggtgatgctg gcccgtgcca gactgacccg gcgcgaggga gacattgaca aggcgacgga
     1681 gatgtttcag gcctacgtca cggccgaagg atcgaccgct caatcgctgc
//
LOCUS       DAORHD010001169         1730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91153_length_1730_cov_3.078209, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001169 DAORHD010000000
VERSION     DAORHD010001169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91153_length_1730_cov_3.078209"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PKO06_19745"
     CDS             complement(<1..687)
                     /locus_tag="PKO06_19745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV71948.1"
                     /translation="MEAAELILKKDKEFGLRRGHYWVYREAVKRIPESLDTGSEVRVV
                     SHKGDDLGRGYVDIDSPILVRRIDGDPELSLDKLIRTRVIDAVRLRRSCFDEKQTTGY
                     RLINGEGDGIPGLVVDRYASALSVQLYSLGLEPWFSAICETLRREISGIRWIYRRNQV
                     RRAKAGGDGLVFGRDFPGSVTFLENGMSFRVDLASGQKTGFFLDQRDNRALIRTVARG
                     KTVANVCGYTG"
     gene            complement(687..1535)
                     /locus_tag="PKO06_19750"
     CDS             complement(687..1535)
                     /locus_tag="PKO06_19750"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNV71949.1"
                     /translation="MSTSHLLFVIALFGAFCLAAPVSAAETPVTIPTATETPAVNPTT
                     PGSTPTPADPAKDATTASSTANASDTTVASGPVGVRVNVFAVVSPDIEEQVKVVGTIL
                     KDQLGMETFAQKGFQIHCTLYMTHFLPEKTERLTQEVASLAATLKTFDVQTTGLMLTK
                     DNWLFLNLEKNKNLNDLMEKVVKGLYNLRSDNIPVPEWLKNYPEKMSSFKVYGSPNVF
                     EDFEPHLTLVAKGDFEKLAKFIVLAKTSDVLMKTVNGRIVGIGVGLADDDGQVQKAQI
                     IFPLKP"
BASE COUNT          369 a          478 c          513 g          370 t
ORIGIN      
        1 accggtatag ccgcagacat tcgcgacagt tttgccccgg gcgacggtgc gaatcaacgc
       61 ccgattgtcg cgctggtcga gaaagaaacc cgtcttctgc cctgaagcga gatcgacccg
      121 gaacgacatg ccgttctcga gaaaggtgac cgaaccggga aagtcgcgcc cgaacaccag
      181 accgtcgccg ccggccttgg ctcgtcgcac ctgattgcgt cgatagatcc agcgaatacc
      241 cgagatctcg cgccgcagcg tctcgcagat ggcggaaaac cagggttcga gcccaaggct
      301 gtacagctgc accgaaagag ccgaggcgta gcggtcaacc accagaccgg gaataccatc
      361 gccctcgcca ttgatcaggc gataccccgt ggtctgcttc tcgtcgaaac acgagcgccg
      421 cagacgcacc gcgtcgatca cgcgggttct gatgagttta tcgagagaca actcggggtc
      481 accatcgata cggcgcacca gaatcggcga atcgatatcg acgtatcccc gccccagatc
      541 gtcaccttta tgggacacaa cccggacttc tgagcccgta tcgagacttt cgggaatacg
      601 cttcaccgct tcgcggtaga cccagtagtg tccgcgacgc aggccgaact ccttatcctt
      661 tttcaggata agttctgcgg cttccattac ggtttaaggg ggaaaatgat ctgggctttc
      721 tgcacctgcc cgtcgtcgtc cgccaggcca acgccgatgc cgacaatgcg gccattcacc
      781 gtcttcatca ggacatcgct ggtcttggcc agcacgatga acttcgccag tttctcgaaa
      841 tcgcctttgg caaccagcgt cagatgcggt tcgaaatctt cgaagacgtt cgggcttccg
      901 tagaccttga agctcgacat cttttccggg tagttcttga gccactctgg aaccgggatg
      961 ttgtcagatc tcaggttgta caaacccttg acgacctttt ccatcagatc gttcaggttc
     1021 ttgtttttct cgagattcag gaacagccag ttgtccttgg tcagcatcag accagtggtc
     1081 tgaacgtcga atgtcttgag cgtggccgcg agagaggcga cctcctgagt gagacgttcg
     1141 gttttttccg gaaggaagtg tgtcatgtac agggtgcaat gaatctgaaa ccctttctgg
     1201 gcgaaggttt ccatgccgag ctgatccttc aggatggtgc ccacgacctt gacctgttct
     1261 tcgatgtcgg gcgaaacgac ggcaaagacg ttgacacgca cgccaactgg gcctgaggcg
     1321 acggtggtat cggaagcatt ggccgtcgaa gaagcggtgg tcgcatcttt ggcggggtcg
     1381 gccggagtgg gagtcgagcc gggagtcgtt gggttcacgg ccggagtctc ggtcgcggtt
     1441 ggaatggtga ccggggtttc tgcggctgag acgggtgcgg ccagacaaaa agcgccgaac
     1501 agggcgatga cgaacaacag gtgtgacgtg gacatgggtt atttctcctt gcgagacacg
     1561 acgtatgcgg gaactatagg acattttcgt tctgatgtcg actgaaaaga gccgcatcgc
     1621 cgagaatctg ccagcggccg cggttcttga cctgtcgtga cgggaagggt aatgtacaac
     1681 gataccgacg tgatgaacgg agagatgtcg tgatgatttc ccgtgttcga
//
LOCUS       DAORHD010001170         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91261_length_1729_cov_2.723417, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001170 DAORHD010000000
VERSION     DAORHD010001170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91261_length_1729_cov_2.723417"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..1695
                     /locus_tag="PKO06_19755"
     CDS             280..1695
                     /locus_tag="PKO06_19755"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV71950.1"
                     /translation="MRNQFLCFRRISVVLAVLMSLLGVAQGLEAREGKSRTRRVTQRS
                     AVMSSSVLTPPTPVNQRYPFLWGVSTAGYQYEGGNDTSVWKRFEETGAVPMKCGRAAN
                     GFELFEADLDRARAMGLNAFRTSIEWGRIEPRQGEIDPAGVAFYHRLFQGMRKRGLTP
                     VVTLVHFSWPQWLEDDLGGWQNPHSLRAFSRYVDFVSKEYGAEVDWWLTFNEPTVIIL
                     GGFATGQLAPGLKKPLLAAKVARHWVEAHKAAYDIIHCNDTQAMVGFNNYTGTYNVLG
                     HDLIHFNIDSRRVSLSTSTLDKPWLELADPMRGKHLDYVGIDYYCRWRLPQAFTPAYT
                     WEPAPEGFYQVIRNYHRVFRVPVLIAENGMATRNLQPRTDGWTREAFLVQHVRQMQRA
                     MREGVPVLGYIHWSITDNYEWGSYEPRFGLYSVECAAEDFRRLPTSAVAVYREIATSG
                     GVSDALAARFPAPVGRSKTGD"
BASE COUNT          358 a          439 c          554 g          378 t
ORIGIN      
        1 cccgagatga aggagaactt caagctgcaa gacacccaca tcggaatcat cgtgcagaac
       61 ggaaattcgg tgcaggctat gagcaattcg agcagtcagc gcatgccgcg cctgcattcg
      121 gcaacctatg ctccggtttc tcgcgtgctg cgcaaagtct gatacgacag agcctctgaa
      181 ccggcgcatc gctgaacatc ggcgatgcgc cggttctgtt tttgtgagat aatgaggcgg
      241 taacacagac cacgggccgg aaacaggcag gaggttggca tgcgaaacca gttcttatgc
      301 ttccgccgca tcagcgtcgt attggctgtg ctgatgagcc ttttgggggt ggcgcagggg
      361 ctcgaggctc gcgagggcaa aagtcgaacg cgtcgcgtga cacagcgttc tgccgtgatg
      421 tcttcgtcag ttctgacacc gccaaccccg gtgaaccaga gatacccatt tctgtggggc
      481 gtgagcactg ccgggtatca gtatgagggc ggcaatgaca ccagcgtctg gaaaagattc
      541 gaagaaactg gtgccgttcc catgaagtgt ggtcgagcgg cgaatggttt cgagttgttc
      601 gaagctgatc tcgatcgagc gcgagccatg ggattgaatg cgtttcgcac cagtattgaa
      661 tggggacgta ttgaaccccg gcaaggagaa atcgatccgg ctggggtggc tttctatcac
      721 cggttgtttc agggtatgcg aaaacgcggt ctgacgccgg tcgtgacgtt ggtgcacttt
      781 tcgtggccgc agtggctcga agatgacttg ggtggctggc agaacccgca cagtttgcgg
      841 gcgttttccc gctatgtcga tttcgtttcg aaagaatatg gcgctgaagt tgactggtgg
      901 ctgacgttca acgaaccgac cgtgatcata cttggggggt tcgcgacggg tcagctggca
      961 ccagggttga agaaaccgct gctggcagcg aaggtggcaa gacactgggt tgaggcgcat
     1021 aaggccgcct acgacatcat tcactgcaac gacacccagg cgatggtcgg gttcaacaac
     1081 tacacgggca cttacaatgt actcggacac gatctcattc acttcaacat cgatagtcgg
     1141 cgcgtgagtc tctcgacgag tactcttgat aaaccgtggc ttgaattggc tgatccgatg
     1201 cggggaaagc acctcgatta tgtcggtatt gactactact gtcgctggcg tctgccccag
     1261 gccttcaccc cggcctatac ctgggaacct gctccggaag gcttttatca ggttatccgg
     1321 aactaccatc gcgtctttcg ggtgccggtg ttgattgcgg aaaacggcat ggcgacgagg
     1381 aatctgcagc cgcgaaccga cggctggacg cgagaggcgt ttctggtgca gcatgtgcga
     1441 cagatgcagc gcgcgatgcg tgaaggggtg ccggtgctgg gatacattca ctggtcgatc
     1501 accgataact acgaatgggg ttcgtatgag ccgcgatttg ggttgtactc ggttgagtgt
     1561 gccgccgaag actttcggcg tctaccgacg tcggctgtgg ccgtgtatcg cgagattgcg
     1621 accagcggcg gtgtcagcga tgcgcttgcc gccagattcc ccgctccggt ggggcggtcg
     1681 aagactgggg actaggggca gggtatggtg gaaccgcatg atctgagtc
//
LOCUS       DAORHD010001171         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91502_length_1726_cov_3.153800, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001171 DAORHD010000000
VERSION     DAORHD010001171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91502_length_1726_cov_3.153800"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..739
                     /locus_tag="PKO06_19760"
     CDS             29..739
                     /locus_tag="PKO06_19760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV71951.1"
                     /translation="MSEDLVVIRELAKQFDQIKVLNKISFSIRRGEIFGLLGLNGAGK
                     TTLFKCILQFVSQDSGEIHYQGFPLTPPTIHRSIGYLPEFFLPPAELSARELLRLLAL
                     GVSGRPADIDALLVKTGLNPDKRIREYSRGMIQRLGLAMALLKNPDFVVLDEPTLGLD
                     PLVRSRLLDWLQEMNQDGKTILLSSHDFSQVEKLCHRVAILHQGVISYIGDIQGFLQK
                     RATNSLETAFLKEVEPHA"
     gene            732..1493
                     /locus_tag="PKO06_19765"
     CDS             732..1493
                     /locus_tag="PKO06_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71952.1"
                     /translation="MRKPLSIAGVTWLQGLRNQTFWIAAFLFAAFLLVAFFLRVLAIG
                     HKAMMLRSFGLASMELSALLLIVYGFVFSMHRERENRIQSLYLTYVSPWQYLLGKLLG
                     NCLLIFAYLSIAAFLCAALLFHEGAFSGAFFFGTYSIFLKLCIICSFCLLFSCLLSSP
                     VFASLMTFFTYCAGELSAYPLMIMKSSTNQMIVTFYRVLYHLLPNMDKVDLKYLAIWE
                     LPIEPMHVLTITVYAFFYSMMLFVPALWAFSRREH"
     gene            1497..>1726
                     /locus_tag="PKO06_19770"
     CDS             1497..>1726
                     /locus_tag="PKO06_19770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71953.1"
                     /translation="MSRIFLALFAVSCIIQLGLSFHLQYVPPNQSLKLHFVPDPQYLK
                     SISGTFRPLLAQAFFMKGALELARNASQKVDYL"
BASE COUNT          363 a          447 c          390 g          526 t
ORIGIN      
        1 gatctgtatt catacaccag aggaaacaat gtctgaagat ttggtagtca ttcgtgaact
       61 tgcgaaacag ttcgatcaaa taaaggtttt aaacaaaatt tcgttcagca ttcgacgcgg
      121 agaaattttc gggttactcg gtctgaatgg tgccggaaaa accacgttgt tcaagtgcat
      181 tcttcagttc gtaagtcaag actcaggcga gattcattac cagggctttc ctcttacccc
      241 gccgaccatt caccgctcca tcgggtatct gccagagttt ttcctgccac cagccgaatt
      301 gagcgcgcgc gaacttctgc gcctgctggc tctcggcgtt tcgggcagac ccgcagacat
      361 tgacgccctg cttgtgaaga ctggcttgaa tccagacaag cgcattcgcg agtattcacg
      421 tggtatgatc caacgcctcg gtctggccat ggcccttctg aaaaatcccg atttcgtcgt
      481 actcgacgaa ccgaccctcg gtcttgatcc acttgttcgc tcacgtcttc tcgattggct
      541 tcaggaaatg aatcaggatg gaaagaccat tctgctcagt tcacatgatt tcagccaagt
      601 ggaaaagctg tgtcatcgtg tcgctatcct tcaccagggc gtgatttcgt atattggtga
      661 tatccagggg tttctgcaaa aacgcgcgac gaactctctc gagactgcgt ttctgaagga
      721 ggtggaaccg catgcgtaaa ccactttcca tcgctggcgt gacgtggttg cagggtctgc
      781 gtaaccaaac tttctggatc gcggcatttc tctttgccgc ctttttgctc gtggcatttt
      841 ttcttcgggt tctggccatc gggcacaaag ccatgatgtt gcgcagcttc ggtcttgcct
      901 ccatggaact cagcgcgctg ttactcatcg tctacggttt tgttttcagc atgcatcgcg
      961 aacgtgaaaa tcgcattcaa tcgttgtatt tgacctatgt atctccatgg caatatctct
     1021 tgggcaagtt gctgggaaac tgtctgctga tcttcgcgta tttgtcgatt gcggcatttc
     1081 tctgtgcagc tctactcttt cacgagggtg cattttctgg tgcctttttt ttcggcacgt
     1141 attccatttt cttgaaactg tgtatcatct gttcgttctg tctgctcttc tcttgcctct
     1201 tgtcatcacc tgtattcgcc tctctcatga ctttcttcac atactgtgcc ggtgagctat
     1261 cggcatatcc gttgatgatc atgaaatcat ccacgaatca gatgattgtc acattttacc
     1321 gtgttctgta tcatctgctg cccaacatgg acaaagtaga tctgaagtat ctggcgattt
     1381 gggaacttcc gatcgagccg atgcatgtgc tcacaatcac cgtgtatgcg ttcttctatt
     1441 caatgatgct gtttgttcca gcgctctggg cattttctcg tcgagaacac tgaaacatga
     1501 gtcgtatttt tctcgctcta ttcgcggttt catgcatcat tcagctcgga ctatcgtttc
     1561 atctgcaata tgttccaccc aaccagtcct tgaagcttca tttcgtgccg gacccacaat
     1621 atctcaaaag tatcagtggg acgttccgcc cactcctcgc acaggctttt ttcatgaaag
     1681 gagctttgga actggctcgc aacgcgtcac aaaaggttga ctatct
//
LOCUS       DAORHD010001172         1725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91625_length_1725_cov_2.616168, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001172 DAORHD010000000
VERSION     DAORHD010001172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91625_length_1725_cov_2.616168"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PKO06_19775"
     CDS             <1..1270
                     /locus_tag="PKO06_19775"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HNV71954.1"
                     /translation="GRRLPREIIRALALIKKAAAMANETVGELVPEKARLIVTAAEEV
                     ADGRWDEHFIVDPLQGGAGTAANMNANEVIANRANELLGNPPGSYNPVHPLEHVNLGQ
                     STNDVFPTAVKLAAIWDLRDLVAEVAHLQEVMQKKEAEFAAVVKIGRTQLQDAVPITL
                     GAEFSAYAEALSRDRWRLYRLEERLRVVNLGGTAVGTGINAGRTFQFELLKTLRDLSG
                     VGLARADNLVEATQNADVFCETSGLLKPLAVTLSKIASDLRLMASGPHSAIGEILLPA
                     VQEGSSIMPGKVNPVMCEMLNQIAFKVYGNDLVITHAAEMGQLELNAFVPVIMDSLLD
                     SFMLLRRGIRLFADRCATGIQANVERCRENLKGSLGILTVLEKKLGHQMTSDIYRRHL
                     ETGQPIVQLLIESGLVTPADIDAAIDAYHL"
     gene            1523..>1725
                     /locus_tag="PKO06_19780"
     CDS             1523..>1725
                     /locus_tag="PKO06_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71955.1"
                     /translation="MDQPTQSQNGTPVTIGTSPGTTVESDLGAQLTRIEEKVGLIEAL
                     QASKRRRVWFFSLAILCCAIWFI"
BASE COUNT          363 a          475 c          531 g          356 t
ORIGIN      
        1 gggacgacgt ctgccgcgcg agatcattcg cgcacttgct ctgatcaaga aagccgccgc
       61 catggcgaac gaaaccgttg gcgaactggt acctgagaag gcgcgcctca tcgtgaccgc
      121 cgccgaagag gtcgccgatg gtcgatggga tgagcacttc attgtcgatc cactgcaggg
      181 cggcgcgggc actgccgcga acatgaacgc caacgaagtc attgcgaacc gagccaacga
      241 gcttctggga aatccccccg gctcgtacaa tcccgttcat ccgctcgaac acgtgaacct
      301 gggtcagagc accaatgacg tgtttccgac cgccgtgaaa ctggctgcca tctgggatct
      361 gcgggatctc gtcgccgagg tggctcacct gcaggaagtg atgcagaaga aggaagctga
      421 gttcgcggcg gtcgtgaaaa tcgggcgcac gcaactgcag gatgcggtgc cgatcactct
      481 gggtgcggag ttttccgcct atgccgaggc actgagccgt gaccgctggc gcctgtatcg
      541 actcgaggaa cgcctgcgcg tcgtgaacct cggcggcacg gcggttggaa cggggatcaa
      601 tgccggtcgc acgtttcagt tcgaactgct caagacgtta cgggatcttt ccggagtcgg
      661 gctggcccgg gccgacaatc tggttgaggc gacacagaat gccgatgtgt tttgcgaaac
      721 ctccggcttg ctcaaaccgc tcgcggtcac cctgtcgaag attgccagcg atctgcgcct
      781 gatggcctcg gggccgcatt cggcgatcgg cgagattcta ctgccggccg tgcaggaggg
      841 gagttcgatc atgccgggaa aagtgaaccc ggtgatgtgc gaaatgctga atcagattgc
      901 gttcaaggtc tatggcaacg atctggtgat cacccacgcc gccgagatgg gacaactcga
      961 gctgaacgcc tttgtaccag tgatcatgga ctctttgctc gactcattca tgcttttgcg
     1021 acgcggaatc agactgtttg ccgaccgctg cgcaaccggc attcaggcca atgtcgagcg
     1081 ctgccgcgaa aatctgaagg gatcgctggg cattctgacc gtgctggaga agaaactggg
     1141 gcaccagatg accagcgata tctatcgacg acaccttgaa accggtcagc ccattgtcca
     1201 attgctcatc gaatcgggtc tggtgacccc ggctgatatc gatgcggcga ttgatgccta
     1261 tcatctgtga gattcaactg ccgatttctg gcgtaagggc gtgcggtgaa gaagcgggtt
     1321 tcgtcgaggg ttatgccctg acagggcctg accacgtttt ctgtctccga agtttcccgg
     1381 aatcgcccgt cgtagtgcga tgcctcggcg aaatcacggc gcggcaggaa atactcctct
     1441 gaaacctgac gatgggagtt gccatggaaa cggcgattcg tgtacagttt gtgcgaggat
     1501 ggaatctgtc agaggagtgg atatggatca accaacacaa tcgcagaatg gaacacctgt
     1561 caccatcggg acttctccgg gcacaaccgt cgagagtgat cttggcgcgc aactgacccg
     1621 aattgaggag aaggtcggac tcattgaggc gttgcaggcc tcaaaacgcc gacgcgtctg
     1681 gttcttcagt ctggcaattc tgtgttgcgc tatctggttc atctg
//
LOCUS       DAORHD010001173         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_91756_length_1723_cov_3.367506, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001173 DAORHD010000000
VERSION     DAORHD010001173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91756_length_1723_cov_3.367506"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="coaD"
                     /locus_tag="PKO06_19785"
     CDS             complement(<1..388)
                     /gene="coaD"
                     /locus_tag="PKO06_19785"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV71956.1"
                     /translation="MARKAVYAGSFDPVTHGHLWMIEQGARMFDELIVAIGVNPDKKY
                     TFSLEERLRVLRASVSGYERVTVDAFENQYLVNYARRIGAAFILRGIRDAHDFEFERG
                     MRHINQDLQPEISTVFLMPPRAIAEVS"
     gene            complement(393..>1723)
                     /locus_tag="PKO06_19790"
     CDS             complement(393..>1723)
                     /locus_tag="PKO06_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNV71957.1"
                     /translation="YSGKPAASAASAVPGAPVADGAKNLQDFAKKIQGFGAGSKDGSV
                     VDLSSLSANDKQSIIQLVNLIIMRALKERASDIHIEPDEKMLRCRFRIDGMLQEVLAL
                     PKALEPACISRCKVMAELDIAEKRVPQDGRIKLQHEGREIDLRVSTYPTLRGEKVVMR
                     ILDKSRVLFGFEELGFADDVLAKFLDLIERPTGIILVTGPTGSGKTSTLYASLQRIKD
                     PTINIVTIEDPVEYMLPGINQGQINPKAGFTFADGLRSILRQDPDVIMVGEIRDYDTA
                     EIAIRAALTGHLVFSTLHTNDAAGAVTRLIDMGVEPFLVASSIIGVLAQRLVRTICDQ
                     CKEEFVPSAGMVKHSKLLYQAGKTKVFRGAGCENCNRTGYRGRTVVTELLLPNEKIKE
                     LIVEKSPSSLIKNESMRMGMRTLRQDGLGKVLRGFTTLEEVIRVSADDEI"
BASE COUNT          359 a          516 c          479 g          369 t
ORIGIN      
        1 aactgacttc ggcaatcgct cgcggcggca tcagaaacac ggtcgagatc tccggctgca
       61 gatcctgatt gatatggcgc atgccacgct cgaactcgaa atcatgcgcg tcacgaatgc
      121 cgcgcagaat gaacgccgct ccgatacggc gcgcatagtt caccagatac tggttctcga
      181 aggcatcaac ggtgacccgc tcatacccgg aaacggaggc acgcaacacg cgcaatcgtt
      241 cttcgagtga gaaggtgtat tttttgtccg ggttcacgcc aatggcaacg atgagttcat
      301 caaacattcg ggcaccctgt tcgatcatcc agagatgccc gtgggtcacc gggtcgaaac
      361 ttcccgcata gaccgctttg cgcgccatgg cctcagatct cgtcgtcggc cgagacgcga
      421 atgacttctt cgagagtcgt gaagcctcgc agcaccttgc ccaggccgtc ctgtcgcagc
      481 gtgcgcatac ccatgcgcat gctttcattc ttgatcagac tcgagggcga cttttcgacg
      541 atcagttcct tgatcttttc attcggaagc agcagctcgg tgaccacggt tcgcccgcga
      601 tatccggtac ggttgcagtt ctcgcaaccg gcgccacgaa agactttcgt ctttccggcc
      661 tggtacagca gcttgctgtg tttgaccatt ccagccgagg gaacaaactc ttccttgcac
      721 tgatcgcaga tcgtacgaac cagtcgctga gccagcacgc cgatgatcga actcgccacc
      781 agaaagggtt cgacgcccat atcaatcaga cgggtgaccg caccggcggc atcgttggtg
      841 tgcagtgtcg aaaacacaag gtgcccggtg agcgccgcgc gaatcgcgat ctcggcagtg
      901 tcgtagtcgc gaatctcgcc gaccatgatg acatctgggt cctggcgcag aatcgagcgc
      961 aagccgtcag cgaaggtgaa cccggctttc gggttgatct gcccctggtt gatgcctgga
     1021 agcatgtatt caaccggatc ttcaatggtc acaatgttga tcgtcggatc cttgatacgc
     1081 tgcagagacg catacagggt cgaggtcttg ccgcttccgg tgggaccggt gacgagaatg
     1141 atgccggtgg ggcgctcgat caggtcgaga aacttcgcca gcacgtcatc ggcaaaaccc
     1201 agttcttcaa agccgaacaa cacccggctc ttgtcgagaa tgcgcatgac caccttttcc
     1261 ccgcgcagtg tcgggtaggt ggaaacgcgc aggtcgatct cgcgcccttc atgctgcagc
     1321 ttgatgcgac catcctgcgg cacgcgcttt tcggcgatat cgagttcggc catgaccttg
     1381 cagcggctga tacaggcggg ttcaagcgct ttcggcagcg ccaggacttc ctgcaacatg
     1441 ccatcgatac gaaaacgaca gcgcagcatc ttctcgtcgg gttcgatatg aatgtccgag
     1501 gcgcgttctt tcaaagcccg catgataatc aggttgacga gctgaatgat gctctgttta
     1561 tcgttcgcgc tcaggctcga aagatcaaca acactgccgt ctttgctgcc cgcgccgaac
     1621 ccctgaatct tcttggcaaa atcctgcagg ttcttggcac catcagcgac cggggctccc
     1681 ggaactgcgc tggcagcgct cgccgccggc ttgcctgagt agt
//
LOCUS       DAORHD010001174         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92107_length_1719_cov_3.362981, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001174 DAORHD010000000
VERSION     DAORHD010001174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92107_length_1719_cov_3.362981"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKO06_19795"
     CDS             <1..1213
                     /locus_tag="PKO06_19795"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV71958.1"
                     /translation="TFAVSLYPTARNFPRFDSYRPYFELLKRARRVRDNVLFHRDSEM
                     AADSELAFLRCSHLKHYTLIKAVPHAQVPMRLSTPRSQVFGFMLGTLLFGVGLFHIFI
                     QRLLVPVREITAAIEAMGRKNFEFRVPVHSGDEMGKLCQALNHALAHLGQMEVASAIQ
                     TALLPRAPYQAGCVFVAGRNQMTQAVGGDYFDLLPLADQRCAVILGDVSGHGVSAAMV
                     AAMAKAGFSLLCPLHPEHPEVVLEKLNHHFLKILDRTKMMTCLIGVVHGASRKLIFSN
                     AGQSYPLLIDSNGNTELYSFASTPLGVRGKAKFVARELDLTKVDIVFYSDGLVEAQTP
                     QGRMVDYEGFARAAQQAYPGDVTAFLERLFLTIRAMTDPVPWSDDASSVLISTRHNSP
                     PGNNRLPEVPD"
     gene            1349..>1719
                     /locus_tag="PKO06_19800"
     CDS             1349..>1719
                     /locus_tag="PKO06_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71959.1"
                     /translation="MMLDSLIAENPVRFLSLFLMGFGLWLFGLLLRTIPVTGKQHHAW
                     HFTAWGCLSLSGAYFFELLRHPNLDVSRFLIPLFELLSQALFLEAGRLALHQRDEDDA
                     PASLTLTPILWWFFLLLIPGV"
BASE COUNT          330 a          526 c          443 g          420 t
ORIGIN      
        1 gacgttcgcc gttagtctgt atccgaccgc gcgcaatttt ccccgattcg attcctatcg
       61 tccgtatttc gaacttctga aacgggctcg ccgagtgcgt gacaacgttc tctttcatcg
      121 agattccgaa atggccgcag actctgaact ggcttttctg cgttgttccc atctgaaaca
      181 ttacacgctc atcaaggcgg ttccgcacgc acaggtgcca atgcgcctgt ccacgccccg
      241 ctcccaggtc ttcggattca tgctcgggac ccttctcttc ggggtcggtc tgtttcacat
      301 tttcattcaa cggctgctcg ttcccgtgcg cgaaatcacc gcggcaatcg aagcgatggg
      361 acgcaagaac ttcgagtttc gcgtgccggt tcattcaggt gatgagatgg gaaaactctg
      421 tcaggctctc aatcatgcac ttgcacacct cggacaaatg gaagtggcgt cagcgattca
      481 gaccgccctt ctcccccgtg cgccgtatca ggcaggctgc gtgttcgtcg ccggacgcaa
      541 ccaaatgacc caggcagtcg gtggcgacta cttcgatctc cttcccctgg ctgaccagcg
      601 ctgtgccgtc attctgggtg atgtttccgg tcatggcgtt tccgccgcca tggtggctgc
      661 catggccaaa gcaggattct cgctgctctg tccgcttcat cctgaacatc cggaggttgt
      721 actcgagaag ctcaatcacc acttcctgaa gatactcgac cgaacaaaaa tgatgacgtg
      781 tctcatcggg gtagtccatg gcgccagccg caaactcatt ttttcaaatg ccgggcaaag
      841 ctacccgctt ttgatcgaca gcaacggtaa taccgagttg tactcttttg cctccactcc
      901 gctcggggtc agaggcaaag ccaagttcgt ggcccgcgaa ctcgacctca cgaaagttga
      961 tattgtcttt tattcagacg gcctcgtaga agcacagaca ccgcaagggc ggatggtgga
     1021 ttatgagggg tttgctcgcg cagcacagca agcgtatccc ggagacgtca ccgcgtttct
     1081 cgaacgactc tttctgacca tccgggccat gaccgacccc gttccatggt ctgacgatgc
     1141 ttcttcggtg ttgatcagca cccgccacaa ttccccgccc ggaaacaatc gcttgcctga
     1201 agtccctgat taagaagata ataccgagga gtgagaagag gcagaaccca cgcatgacgt
     1261 atgaccgcac gacagctgca gccgaagtat ttcgccccct gtgccgggtg gtctctcgcc
     1321 gctctgcggc aggggccgga ctccagccat gatgcttgat tccctgatcg cggaaaaccc
     1381 ggtgcgcttt ctgtcgctct ttctgatggg atttggcctt tggctgtttg gcctgctcct
     1441 gcgcacgatt ccagtcaccg ggaaacagca tcatgcatgg catttcacgg catggggctg
     1501 tctcagcctg agtggcgctt atttttttga actgctccgc catcccaatc tggacgtcag
     1561 ccgcttcctt atcccgctgt tcgaactgct ttcccaagcc ctgtttctgg aagccggccg
     1621 actggcactg caccaacgtg acgaggatga tgctcctgcc tctctcacgc tcacaccgat
     1681 actctggtgg tttttcctgc tgctgattcc gggagtctg
//
LOCUS       DAORHD010001175         1718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92206_length_1718_cov_3.012628, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001175 DAORHD010000000
VERSION     DAORHD010001175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92206_length_1718_cov_3.012628"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="csm5"
                     /locus_tag="PKO06_19805"
     CDS             <1..858
                     /gene="csm5"
                     /locus_tag="PKO06_19805"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HNV71960.1"
                     /translation="VDVAAGLVETYRRVLSMNAFDKRAVINNFMLNRVSFVPGSNEPY
                     VPGTSLKGAIRTALLSKIARDAKPPVVNWRDRAGQLEDKLVEREGRNHFESDPFRLVK
                     VSDFHAVGKVATRIVYAINRKKAPTNRPAREQTGPQQIFEVIQPGAIFEGSLAIHPLV
                     PGAGIHHPLDRATLFESLNTYFNIKLEQEIKTMKGIGCNPLVINIINRDFKGKLTKTA
                     FLTRMGRHSGAECVTIDGNRKIRIMQGPGRPPRYDQQATTIWLSADQPRPNDVKTSEP
                     FGWCVLEVV"
     gene            complement(938..>1718)
                     /locus_tag="PKO06_19810"
     CDS             complement(938..>1718)
                     /locus_tag="PKO06_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71961.1"
                     /translation="EGIRLIAQAAGKRLTFDFKGADHLLTQALGSFKTVSQEQLLRQW
                     LSQLAVLSGRNTQDMSRARTMLMRELVANLIIKVRCEEWVDFLGRWYRLIEEAMKQFI
                     AQYLGLALIFDDRQTQYSDYIHALESRTDVHEFFSVRKASGRTLNWREINTKTGEAWL
                     EFIDLHSPTTSVDLAERARKFLVMLKRYSGLISLRNKCILAHGNQGVGRNEILDALNG
                     TSIELFQKELCQVVLGLDDPDAILSDFQAMSELILKTLKTT"
BASE COUNT          401 a          434 c          492 g          391 t
ORIGIN      
        1 gtcgatgtgg cggctggtct ggttgaaacc tatcgacggg tactcagcat gaatgcgttc
       61 gataagcgcg ccgtgattaa caacttcatg ttgaatcgcg tctcgtttgt cccgggaagc
      121 aacgaaccgt atgttcccgg gacatcgttg aagggcgcga ttcgaaccgc cctgctgagt
      181 aaaatcgctc gcgatgccaa accgcccgtt gtgaactggc gtgatcgggc cggtcagctt
      241 gaagataaac tggtggagcg tgaaggccgc aatcactttg agagtgaccc ctttcgactg
      301 gtgaaagtgt ctgactttca tgcggtcgga aaagtggcga cgcgcatcgt ctacgcgatc
      361 aatcgcaaga aggcgccgac aaatcgaccc gcccgcgagc agacgggtcc tcaacagatc
      421 tttgaagtga ttcagccggg ggcgatcttc gagggcagtc tggcgattca ccctctggtg
      481 ccaggcgcag gcattcatca tccgctggat cgtgccaccc ttttcgagtc gctgaatacc
      541 tacttcaata tcaagcttga gcaggaaatc aagacgatga agggcattgg ttgcaacccg
      601 ctggttataa acatcatcaa tcgcgacttc aagggcaagc tcacgaagac cgcgtttctg
      661 acccgtatgg ggcggcattc cggtgctgaa tgcgtcacta ttgacggcaa tcgcaaaatc
      721 aggatcatgc aggggcccgg acgaccgcca cggtatgatc agcaggccac cacgatctgg
      781 ctgagcgctg atcagccgcg accgaatgat gtcaaaacct cagagccttt cgggtggtgt
      841 gtgttggaag tcgtttgaat gtccgagtat tggtgaatga aaacgcccgt cggaagatcg
      901 aatccggcgg gcgttttgat gagaaaaaag aagcgactca ggtggtcttg agggtcttga
      961 gaatcagctc gctcattgct tggaaatcag agagaatggc gtccgggtcg tcgagtccga
     1021 gaacgacctg acaaagctct ttttgaaaga gttcaatgct ggttccgttg agggcatcga
     1081 ggatctcatt acgacccact ccctgattgc cgtgtgccag aatgcacttg ttacgcagtg
     1141 agatcagacc gctgtatcgt ttgagcatca ccagaaactt tctggctcgc tctgcgagat
     1201 cgacagaagt tgtcggcgaa tgcagatcta tgaactccag ccaggcttca ccggttttcg
     1261 tattgatctc gcgccagttg agcgttcgac cggaggcttt tcttacggaa aagaactcgt
     1321 gcacgtcggt gcgggattcg agggcgtgaa tatagtctga atactgcgtt tgacgatcgt
     1381 cgaagatcaa tgccagacca agatactgag cgatgaactg tttcatcgct tcttcgatga
     1441 gtcgatacca tcgcccaagg aagtcaaccc attcttcaca ccggaccttg ataatgagat
     1501 tggcaaccag ttcgcgcatg agcatggtcc gtgctctact catatcctga gtgttacgac
     1561 cggagagaac agcaagctgt gagagccact gtcgaaggag ctgttcctgg gaaacggttt
     1621 taaaactgcc gagtgcctgc gtcagcagat ggtcggcacc cttgaagtcg aaggtcaggc
     1681 gcttgccggc cgcctgggcg atgagcctga tgccttct
//
LOCUS       DAORHD010001176         1716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92361_length_1716_cov_3.953642, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001176 DAORHD010000000
VERSION     DAORHD010001176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92361_length_1716_cov_3.953642"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PKO06_19815"
     CDS             <1..212
                     /locus_tag="PKO06_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNV71962.1"
                     /translation="VAVLQHELDQGLDGVSEKEVHSRVARIEQILNTYQRGKKFYAHL
                     YDDVLSLKYMYQRYTNYLRWLLTRS"
     gene            212..1219
                     /locus_tag="PKO06_19820"
     CDS             212..1219
                     /locus_tag="PKO06_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71963.1"
                     /translation="MFAPLLGFLQDRRDSLNETFFRLRQQYPGLEQTVGYELVRLVID
                     PLWQSVDGKESEGALLQAAQIWYELGLELAGRGFGPSGHTSAFWPAWLRLGRAYPALV
                     IRQPRPFLIALANALWQLQSTGQPAVERWLGTLENLTDLGNDLSTWMQAGTLTAWRCG
                     LATQRKRALAEARKLSPALGLACLGLSRDLDSAQWLALVERLTIDPWYDPEFDSSWKQ
                     RNPTLIGTVGRFRGLGGEFARPPRVRIWNDVVLAGVEQEWFVVHADVYGTCLQRVTVD
                     LPAETTTLVEPWKLAGGGVVTRQGQQSVIPELGHCAGVAATRDLLVAALHHSHRLFLV
                     V"
     gene            1225..>1716
                     /locus_tag="PKO06_19825"
     CDS             1225..>1716
                     /locus_tag="PKO06_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71964.1"
                     /translation="MTTVAMGRTQNGGPISEPTESAAPVASSFEDLRAAWLAVWPEAM
                     EIWSRFTRLREPIWCWTPREEKDQGLTDSFAMIRLTDHLVVISLRQVQQQKLEAFGRE
                     VLAHEIGHHMMCPANLTDHGRLIARIRLGLPKIEHLAPMVANLYADLLINHHLQKEAG
                     LNIA"
BASE COUNT          321 a          487 c          529 g          379 t
ORIGIN      
        1 cggtcgccgt gctgcagcac gaactggacc agggcctcga tggcgtttcc gaaaaggaag
       61 tgcattcgcg tgttgcccgt atcgaacaga ttctgaacac ctatcaacgt gggaaaaagt
      121 tctatgcgca tctctacgac gatgttctga gtctgaaata catgtaccag cgttacacca
      181 actacctgcg ctggttgctg accagaagct gatgtttgcg ccactgctcg gttttcttca
      241 ggatcgtcgt gattcgttga acgagacgtt tttccgcctg cgtcagcagt atcccggcct
      301 ggaacaaact gttggctacg agctggttcg tctggtcatc gacccgctct ggcagtccgt
      361 tgatggaaaa gaatccgaag gagccttact gcaggcggct cagatctggt atgaactggg
      421 actcgaactc gcgggtcgcg gatttggtcc gtctggccac acctcggcgt tctggccggc
      481 ctggctccgg ctcggtcggg cgtatccggc gctggtgatt cgccagccgc gaccgttttt
      541 aatcgctctt gccaatgcgc tctggcagct gcaaagcact gggcaacccg cggttgaacg
      601 ctggcttggc acgctcgaaa accttaccga cctcggcaat gatctctcca cctggatgca
      661 ggccggcact ctgactgcct ggcgctgtgg gctggcgacg cagcgtaagc gtgccctggc
      721 ggaggctcgc aagctgtctc cggctctcgg gctggcctgt cttgggttgt cacgtgacct
      781 tgactcggct cagtggctcg cgctggtcga gcgattgacg atcgatccct ggtatgatcc
      841 ggagttcgac agctcatgga aacaacgaaa cccgacgctg atcggaaccg ttggtcgctt
      901 tcgtggtctg ggtggagagt tcgctcgtcc gccacgggta cgaatctgga acgatgtcgt
      961 gctggccggg gtcgagcagg aatggttcgt agtgcatgcg gatgtctatg gaacttgtct
     1021 acagcgcgtg acggttgatc ttcccgcgga gacgacaacg ctggttgaac cgtggaagtt
     1081 ggcaggcggg ggtgtggtca cccggcaggg acaacaatcc gtgattcccg aactgggaca
     1141 ctgcgccggt gttgccgcca cgcgtgatct gctggtcgcg gcattgcatc actcacatcg
     1201 cctgtttctc gtggtttgaa acccatgacg actgtcgcga tgggaagaac gcagaacgga
     1261 ggtccgatct cggagccgac cgaatccgcg gctcccgtgg cttcgagttt cgaggacctg
     1321 agagccgcct ggctggcggt gtggcccgaa gccatggaaa tctggagtcg gttcacccgt
     1381 ctgcgcgaac cgatctggtg ctggactcct cgcgaagaga aagatcaggg tctgaccgac
     1441 agcttcgcca tgattcgtct gaccgatcat ctggtggtga tcagtctgcg acaggtgcaa
     1501 cagcagaaac tcgaagcctt cgggcgcgag gtgctggccc acgagatcgg tcatcatatg
     1561 atgtgtcccg ccaatctcac cgatcacgga cgtctcatcg cgcgcattcg tctcggtctg
     1621 ccgaagatcg agcacctggc gccgatggtt gcgaatctct atgccgacct gctgatcaat
     1681 catcatctgc agaaagaggc gggactgaat atcgcc
//
LOCUS       DAORHD010001177         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92660_length_1713_cov_3.442702, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001177 DAORHD010000000
VERSION     DAORHD010001177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92660_length_1713_cov_3.442702"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PKO06_19830"
     CDS             complement(<1..420)
                     /locus_tag="PKO06_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71965.1"
                     /translation="MTPPAHVLFRIEAGNTIPEEVWIQAGIAGLVMMGLIFILYSLFL
                     KNRLFPWWFGGPRQLILRELDQNSWEAIFEGAGLGAGRSTSFEYVLEVGSAATHTKYS
                     PKHTVTTTIGENRRFYRLLPTATPTTSFLVRVPIPPES"
     gene            complement(514..>1713)
                     /gene="ftsY"
                     /locus_tag="PKO06_19835"
     CDS             complement(514..>1713)
                     /gene="ftsY"
                     /locus_tag="PKO06_19835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNV71966.1"
                     /translation="PESLAEPVQREALQEKPAGQVIILPPVPEPLTPVSPVPPTEPAR
                     PIPPVEEKPPAKPIETPAAPPPVAEKKVEPKPAEDDDAQLSWYDRLRRGLSKTRNSFV
                     GKLKKLVFGTTKIDEDMLEELEETLFEADVGPKTTTELLEIIHKEVEKKEVKDPSFVY
                     QTLRDELKKRLNRFVSQVKWNPDGLTVFLIIGVNGVGKTTTIAKLAHRFKKEGKNVVL
                     AAGDTFRAAAIDQLGIWADRAGVEVIKGQEGGDPGALVFDAIHAARKRNANLLIVDTA
                     GRMHVKANLMDELRKIRKIVEKEAAGPHETLLVLDANTGQNAIQQAKLFNEALPISGL
                     VMTKLDGTAKGGVLFAVEEQLNVPVKFIGVGEKMNDLMDFNPDQFLEALFADDTDKSK
                     QSDYNVL"
BASE COUNT          332 a          460 c          494 g          427 t
ORIGIN      
        1 tgactctggc ggaattggga ctcgcacgag aaaggaggtc gtcggtgtcg ctgtcgggag
       61 gaggcgatag aatcgtcgat tttcgccaat ggtggtcgtg accgtatgtt tcggagaata
      121 ctttgtatgg gtggcggctg aaccgacttc gagaacatac tcaaagcttg tgctgcgtcc
      181 ggctcctagt cccgctccct caaagattgc ttcccaggag ttctggtcga gctctcgcag
      241 gataagctgg cgtggcccgc cgaaccacca gggaaagagt cggtttttca aaaacaggga
      301 gtacaggatg aagatcagac ccatcatcac caaaccggca ataccagcct gaatccagac
      361 ttcttccggg atcgtgttgc ctgcttcgat gcggaaaagt acatgtgcgg gaggcgtcat
      421 acaagcttct cctctaagaa aaggcggtga tgaaaaacgg gcttacccac atactgtgct
      481 tggtaagccc gtttcatgcg gcggttattg tctctagaga acgttataat cgctctgttt
      541 tgacttgtcg gtgtcgtcgg cgaagagcgc ttcgaggaat tggtcgggat tgaagtccat
      601 cagatcgttc atcttttcgc caacgccgat gaacttgact gggacgttca actgttcttc
      661 gaccgcgaac agcacgccac ccttggccgt tccgtccagt ttggtcatga ccagaccact
      721 gatcggaaga gcctcattaa agagcttcgc ctgctgaatc gcattttgcc ccgtattggc
      781 atcgaggacc agcagtgtct cgtgcgggcc agcagcctct ttctcgacga tcttgcgaat
      841 cttgcgcagc tcgtccatca ggttggcttt cacgtgcatg cgcccggcgg tgtcgacgat
      901 taacagattc gcgttgcgtt tgcgggccgc gtgaatggca tcgaagacca gagctccggg
      961 gtcaccgccc tcctgcccct tgatgacctc gaccccggcg cgatcagccc agatcccaag
     1021 ctggtcaatg gccgcggcac ggaacgtgtc gcccgcggcg agaacgacgt tcttcccttc
     1081 cttcttgaag cgatgggcaa gtttggcgat ggtggtcgtc ttgccgacgc cgttgacacc
     1141 gatgatcaga aacacggtca ggccatccgg gttccatttg acctggctga caaaacgatt
     1201 aagtcgcttc ttgagctcat cacgcagggt ttgatagacg aaagacgggt ctttgacctc
     1261 tttcttttcc acttccttat ggatgatctc gagcagctcc gtcgtggtct tcgggcctac
     1321 atcagcttca aacagcgttt cttcgagctc ttcgagcatg tcctcgtcaa ttttcgtggt
     1381 gccgaacacc agttttttca gtttgccgac aaagctgttg cgcgtcttcg ataacccgcg
     1441 tcgcaaacgg tcataccagg aaagttgcgc atcgtcgtct tcggcgggct ttggttcgac
     1501 cttcttttcg gcgaccggag gaggtgcggc aggggtctcg atcggtttcg cgggcggttt
     1561 ctcttcgaca ggggggatcg gtctggctgg ttcggtcggc ggaaccggag aaaccggcgt
     1621 gagaggttcc ggtactggtg gtagtatgat gacttgtcct gcaggttttt cctgcagcgc
     1681 ttctcgttga actggttccg cgagagactc tgg
//
LOCUS       DAORHD010001178         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92670_length_1713_cov_3.083233, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001178 DAORHD010000000
VERSION     DAORHD010001178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92670_length_1713_cov_3.083233"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PKO06_19840"
     CDS             <1..696
                     /locus_tag="PKO06_19840"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV71967.1"
                     /translation="LVLSSAVTFGILTFIVPRFKDIYKRFGSELPWLTQTTISLSDLM
                     VSHFLLFFGGTVATLFLLAALKRTESGKRVFDRLLLAVPVIGHMYHLYEIASFSKSFS
                     ILLHSGITVTTALDIIVPGIDLQPVRERIQAANSEIRNGRPMGESFALQEPLLPDLLN
                     RMVLVGERTGNLTEMLDHVAEFYEEEFYNQVERLASLIEPMLIVFLGVIIGGIVISLY
                     LPIFGMAKLIARR"
     gene            700..>1713
                     /locus_tag="PKO06_19845"
     CDS             700..>1713
                     /locus_tag="PKO06_19845"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71968.1"
                     /translation="MEARDLIRLLSGDNPKLQLYALKEWENSHRHDPEPLLTALDVSE
                     TEVVHRALTLATRYHPTACAAKVIRLTEAADARFRRMAVEHLAPAMGQPAQQALTSLL
                     AKERDPFVLASAVTTAARLRLAASLLKPFLTHQEIRLRSNTIRALGALGSPEIRPLVE
                     PALKDPALRVQGEALIALAPLISPADLLAHIRRRLESPDSMVRAATIIITAQLPLTQR
                     LVLILERLQDPELRVVHCALRALAQLQDPTAIQHLIECYFSIDHPDTRKLLLQSLATM
                     PSRLTLAQFVQIAPAAQVDSTRLSRILTLIGRLPDWEPFLPWLLNALERSDDGLRLQG
                     LELV"
BASE COUNT          327 a          559 c          448 g          379 t
ORIGIN      
        1 ctcgttcttt catcggcggt caccttcggc attctcacct ttatcgttcc ccgcttcaag
       61 gatatctaca aacgtttcgg cagcgaactt ccctggctca cccagacaac gatttctctc
      121 agcgacctta tggtaagcca ctttctgctg ttcttcggtg gtacggtcgc caccctgttc
      181 ctgcttgccg cgttgaaacg caccgagtca ggaaagcgcg tctttgatcg actgctcctc
      241 gctgttccgg tcattggcca catgtatcac ctctacgaga tcgcctcatt ttccaagagc
      301 ttttcgattc tgctgcacag tgggatcacc gtgaccaccg ccctcgacat catcgttccc
      361 ggcatcgatc tgcaaccggt tcgagaacgc attcaggcag ccaacagcga gattcggaac
      421 ggacgaccga tgggcgaaag cttcgcgctg caggagccac tgctgcccga cctgctcaat
      481 cgcatggtcc tggtcggaga acgcactggc aacctgaccg agatgcttga tcatgtggcc
      541 gagttttatg aagaggagtt ctacaatcag gtcgaacggc tcgcctcttt gattgaaccg
      601 atgttgatcg tgtttctggg agtcatcatt ggcggcatcg tgatctctct ctatctgccg
      661 atcttcggca tggccaaact gattgcccgg cgctgagaca tggaagcacg tgatctgata
      721 cgcctgctgt ccggcgacaa ccccaagctg cagctctacg cgctgaaaga gtgggaaaac
      781 tcgcatcgac acgatccgga accccttctg accgccctgg atgtgagtga aaccgaagtg
      841 gttcatcgag ccctgacgct tgccacccgg tatcacccga ccgcctgcgc cgccaaggtc
      901 attcggctca ccgaagcggc agatgcacgt ttcaggcgca tggcggtcga gcatctcgct
      961 ccggcgatgg ggcagccggc gcaacaggcc ctgacttcgc tcctggcaaa agaacgggat
     1021 cccttcgtgc tcgcctccgc cgtcacgact gccgcccgtc tgcgcctcgc ggccagtctc
     1081 ttgaaaccct ttctgacaca tcaggagatt cgcctgcggt cgaatacgat tcgcgccctc
     1141 ggtgccctgg gcagcccgga aattcggccg ctggtcgagc ccgcgctgaa agatccggct
     1201 ctgcgcgtgc agggagaagc tctgatcgca ctcgcaccgc tcatctcacc cgccgatctt
     1261 ctggcccaca ttcgccgccg actcgagagc ccggactcga tggtacgagc cgctacgatc
     1321 atcattacgg ctcaactgcc gctgacgcag cggctggttt tgattctcga acgactgcag
     1381 gatccggaac tgcgcgtcgt ccactgcgcc ctgcgagctc tggcgcagtt gcaggacccc
     1441 acagccattc agcacctgat cgaatgttac ttcagcatcg accaccccga caccagaaaa
     1501 cttctgttac agagtctggc cacgatgccc agtcgtctga cactcgctca atttgtccag
     1561 attgcccccg cggcacaagt cgatagcacg cgtctttcac gcattctcac gctcatcggc
     1621 agactccccg actgggaacc gtttcttccc tggctactga atgctctgga gcgttctgac
     1681 gatgggcttc gactgcaggg gttggaactg gtt
//
LOCUS       DAORHD010001179         1713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92715_length_1713_cov_2.103136, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001179 DAORHD010000000
VERSION     DAORHD010001179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92715_length_1713_cov_2.103136"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..404)
                     /locus_tag="PKO06_19850"
     CDS             complement(102..404)
                     /locus_tag="PKO06_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71969.1"
                     /translation="MMCSTLGALYKLNKQVLYVYEDEAEKQPQPNIVVAPIDAPQNTL
                     SVKRAGPMPSFAFTPVDEPDLVQQFKQGDERRRGLKELAQLASAANAIVPVKEEKK"
     gene            complement(448..1473)
                     /locus_tag="PKO06_19855"
     CDS             complement(448..1473)
                     /locus_tag="PKO06_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71970.1"
                     /translation="MRLFKVAFFEEFTKNLTLFNWTFMISPPSIPLIYRREERKPFTF
                     LNRQISGNLRQGQVGRILQSDDPENYLMSRAVCGIPANRLLDQPVAVRDPDGGAAPNF
                     LTQYNPLSEGPFPDPQQIPGPKGKRAGERFFPHIDYSTFSHNYKTPQEFLNERVTLEN
                     GEPVLYIDGRMYIAEGDLDLVAGWHGRPVKKYKGRGFLFLSKGNVTLTSFRKGGSPSS
                     DDSLRIFLQDGDFIVGGSDAPTNIEASLIAVTYLYDAAKDPKELTNQGQLLMRGKSVV
                     VTGNLVVDYLALELGNDGLPMNGTLTIVHDPEIFEPANGEDNPYRFSIGEVRTFYSMN
                     SGDPTLN"
BASE COUNT          387 a          477 c          440 g          409 t
ORIGIN      
        1 cgtccgcgtc gaggtcacca gatagaacag cggcacagcg agcacggctg caaccagaat
       61 cgcaaccatg atttcggcga atgtaaatcc agctcttcga ctcacttttt ttcttccttg
      121 actggtacta tcgcattggc cgcagatgcc agctgcgcca gttccttgag cccgcggcgc
      181 ctctcgtctc cctgcttgaa ctgttgcacc agatcaggtt cgtcaaccgg cgtgaacgcg
      241 aaagacggca tcgggccggc tcgcttgacc gacagtgtat tctgcggtgc atcgatcgga
      301 gccacgacga tgttcggctg tggctgcttc tctgcttcgt cttcgtacac gtagagcacc
      361 tgcttgttca atttgtacaa ggcacccaga gtgctgcaca tcatcagcac tacaactccc
      421 acgataatcc agcgtgcggc atccatatca gttaagcgtg gggtctcctg aattcatgct
      481 gtagaacgtg cgaacctcgc caatgctgaa gcgatacgga ttatcctcgc cattggcagg
      541 ttcgaagatt tccgggtcgt gaacgatggt cagcgtccca ttcatgggca ggccatcatt
      601 gcccagttcc agagcgagat aatccacgac aagattaccg gtgaccacga cgctcttgcc
      661 gcgcatgagc agctggccct gattcgtcag ttccttcgga tctttcgccg catcatacag
      721 gtacgtcacg gcaatcagcg aagcctcgat attcgttggc gcatcggaac cgccgacgat
      781 gaagtcccca tcctgcagga agatgcgcag cgaatcatcc gatgacggag acccaccctt
      841 gcggaaactc gtcagtgtga cattcccctt actcaggaac aggaacccgc gacccttgta
      901 tttcttgacc ggacggccat gccaaccggc aaccagatca agatcgcctt cggcgatgta
      961 catgcgcccg tcgatgtaga gtaccggttc tccgttctca agagtcactc gctcgttgag
     1021 aaattcctgg ggtgttttgt aattatgcga aaacgtcgaa taatcgatgt gcggaaagaa
     1081 ccgctctccg gcacgtttcc ccttcgggcc gggaatctgc tgaggatcgg gaaatggtcc
     1141 ctcactcagt gggttgtact gtgtcagaaa attcggtgcg gcccctccgt ccgggtcacg
     1201 cacggctaca ggttggtcca ggagcctgtt tgcgggaatt ccacagacag cccggctcat
     1261 gagataattc tctgggtcat cactctgaag aatccgtccg acttgcccct gtctgaggtt
     1321 ccctgaaatc tggcgattga ggaatgtgaa gggtttgcgt tcttcgcgtc tatagatgag
     1381 aggaatactg ggtggcgaaa tcatgaatgt ccagttgaaa agcgtaaggt ttttcgtgaa
     1441 ctcctcgaaa aacgcaactt tgaatagtcg caagaatact tttccttcaa taaatacagg
     1501 cttgtcgcct gcaactccat agagggatgc catgcgaccg acgaacattt tctcaaggtt
     1561 ccaaggctgc cctgtttgag cctcttcgct ttctgcaaat gagttttcgt aggcgtaacg
     1621 actcaatttg ttcatctcta aactggaagt cgaaagccca caataacggt tcacgaggcc
     1681 ttctgccgta cgatcaggta gaaccaagtc agg
//
LOCUS       DAORHD010001180         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_92902_length_1711_cov_2.684179, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001180 DAORHD010000000
VERSION     DAORHD010001180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92902_length_1711_cov_2.684179"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PKO06_19860"
     CDS             <1..345
                     /locus_tag="PKO06_19860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71971.1"
                     /translation="AVHVTMGKDRNELLDYFFKWGVADELLAFQVKGSKKEKHLTPFE
                     LADRFKPQLWVFGHHHQWFEVQRGLTRFQGLPECWHGYAVWSEKQGFVVVENFIKRHK
                     GFWQNLFPSLFG"
     gene            complement(293..715)
                     /locus_tag="PKO06_19865"
     CDS             complement(293..715)
                     /locus_tag="PKO06_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HNV71972.1"
                     /translation="MTQTRGKLEARISEAVIQFEREFMGRGPLEAKTYLIDDMVIVRL
                     KGVLTPAEHQLAKTVPESDGRALIKKMRETLIEKERLLYQMRLGATGSCLVVVMGQSD
                     RNRISGLQLLHGNHQRRSRRSLTRTSLGRGSARSPCGV"
     gene            complement(791..1276)
                     /locus_tag="PKO06_19870"
     CDS             complement(791..1276)
                     /locus_tag="PKO06_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011964049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HNV71973.1"
                     /translation="MSEAAFTIGANPATTGAVSPRDFFTPEQVRELEKAIRGAESRTS
                     GEIRIHLESACADPIVRATEVFEQLGMSKTRHNTGVLIYVAIVSQQFTIIADSGISRK
                     VPGDFWDNIRDIMEAEFRQGRFLNGLRFGIEVAGIELAKFFPRPDDDTNELPDTLSYG
                     N"
     gene            complement(1273..>1711)
                     /locus_tag="PKO06_19875"
     CDS             complement(1273..>1711)
                     /locus_tag="PKO06_19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNV71974.1"
                     /translation="EIRHGAYIRGHVIIGPGCIVGHCSEINRSLMLPEAKAPHFNYVG
                     DAILGSRVNLGAGTKISNLKNDGTPVKVSVGGTIYNTGLRKFGAILGDGCMLGCNSVT
                     NPGTVMAPNGRAYPNATISGYHPPGTIVKLRQQLETVLVEAKP"
BASE COUNT          328 a          481 c          518 g          384 t
ORIGIN      
        1 gcggtgcacg tcacgatggg caaggatcgc aacgaattac ttgactattt tttcaagtgg
       61 ggcgttgctg atgagctgct ggcgtttcag gtcaaaggct caaaaaaaga aaaacacctg
      121 accccgttcg aactggctga ccggttcaag ccgcagttgt gggtgttcgg tcaccaccat
      181 caatggttcg aggtgcaacg tggtttgacc cgatttcagg gcttgcccga atgctggcat
      241 ggctatgcgg tatggtctga aaaacagggg tttgtcgtcg tcgagaactt catcaaacgc
      301 cacaagggct tctggcagaa cctcttccca agcttgttcg ggtgagcgac cgacggcttc
      361 tccgctgatg attgccgtgt agtagctgta ggccagaaat ccgattccga tcagactggc
      421 ccataaccac cacgagacag gagccggtcg ctccgagccg catttgatac agaagccgct
      481 ctttttcgat cagggtctcg cgcatcttct tgatcagggc ccggccgtcg gattccggca
      541 ccgtcttggc cagctgatgc tcggcggggg tcagcacccc cttgaggcgc acgatcacca
      601 tgtcgtcgat cagatacgtt ttggcctcaa gcgggccgcg acccatgaac tcgcgttcga
      661 actgaatgac agcttcgctg atgcgcgctt ccagctttcc cctggtttgt gtcatgtggg
      721 tttcagtcgt atccgtcggg tctgtcggcg tcgacgtgac tgtcccagga acagaaagac
      781 aacacgtgca tcaattgccg tagctcaggg tatcgggaag ttcgttggtg tcgtcgtcgg
      841 ggcgggggaa gaacttggcc agctcaatgc cggccacttc gatgccgaag cgcaaaccgt
      901 tcagaaaacg gccctgccga aactccgctt ccatgatgtc gcgaatgttg tcccagaaat
      961 caccgggcac tttgcggctg atgcccgagt cggcgatgat ggtgaactgc tgtgacacga
     1021 tcgcgacgta gatcaatacg ccagtgttgt gacgcgtctt gctcatgccg agctgttcaa
     1081 acacttcagt cgctcgtaca atcgggtcgg cgcaggctga ttcgaggtga atgcgaatct
     1141 cgcccgatgt gcgcgattcc gcgccccgaa tggctttttc gagttcgcga acctgctcgg
     1201 gggtgaagaa atcgcgcgga ctgaccgctc cggtcgtcgc ggggttcgct ccgatggtga
     1261 aagcggcttc gctcatggtt tcgcctcgac cagcacggtt tcgagctgct gccgcagttt
     1321 gacgatcgtg cccggcgggt gatacccgct gatggtggcg ttcggatacg cgcggccgtt
     1381 cggggccatg accgtgccgg ggttggtgac cgagttgcag cccagcatgc agccgtcgcc
     1441 cagaatcgca ccgaacttgc gcagcccggt gttgtagatg gtgccgccaa cgctgacttt
     1501 gaccggtgtg ccgtcattct tcagattgga aatttttgta ccggctccga ggttcacgcg
     1561 cgagcccaga atcgcatctc cgacgtagtt gaaatgcgga gctttggcct cgggcagcat
     1621 cagtgaacga ttgatctcgc tgcagtgacc gacgatgcac cccggcccga tgatgacatg
     1681 gccgcgaatg taggccccgt gacgaatttc g
//
LOCUS       DAORHD010001181         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93156_length_1708_cov_3.750151, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001181 DAORHD010000000
VERSION     DAORHD010001181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93156_length_1708_cov_3.750151"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="PKO06_19880"
     CDS             complement(<1..1258)
                     /locus_tag="PKO06_19880"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HNV71975.1"
                     /translation="MSTTSSIVTSRSVPTLTGDLWGGLASMLVALPASIAFGVLIYSS
                     LGANFVAHGAMAGILGAAAIGIVAPLVGRNGVLISAPCAPAAAVLAAVTAELLSAGTL
                     NITAATIPLLLGLIVLGAGLLQILYGLLGCGRLIKFIPYPVVTGYLSAVGLLIILGQL
                     AKLTGLPRGTPLGEGLITPSLWKWPSVLVGLTAMLVMVHGQRLTKRIPAPVLGLGAGI
                     LAYFVLALFDRTLLQTEGNSLIVGPIQLSGSFLDLMTGRLRSLGDLRFDLLRLVIVPA
                     TTLSILLSIDTLKTCVVMDALSGARHNSDRELIAQGTGNVAAFAAGGMAGAGTMGPTL
                     VNLSSGGQSWRSAAIEGAAVVAILAFVPGLVTWVPIGALAGILLVVAWRMIDRSMFRL
                     LATPAGRQDFAVIATVVVTALGVDLIA"
     gene            complement(1430..>1708)
                     /locus_tag="PKO06_19885"
     CDS             complement(1430..>1708)
                     /locus_tag="PKO06_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71976.1"
                     /translation="SGALHLGRLAFMDRVLVYDRQKLSLYSCSRGIVFPREWELPSGQ
                     VEPLTRVDAEFLDVCRQMFASSRLPEKVIAAWKTALAPSTPPSLPQSA"
BASE COUNT          386 a          515 c          551 g          256 t
ORIGIN      
        1 cggcgatcag atcgacgccc agcgcggtca caacgaccgt ggcgatcacg gcgaaatcct
       61 gtcgaccggc cggtgtcgcc agcaggcgaa acatgctgcg gtcgatcatc cgccaggcaa
      121 cgaccagcag aattccggcc agtgcaccga tggggaccca ggtaaccagg ccgggcacga
      181 acgccagaat tgcgacgacg gcagcccctt cgatggcggc agaccgccag ctctgacccc
      241 cgctggacag gttcacgagg gttggaccca tggtgccggc gcctgccatg cccccggctg
      301 cgaaggcggc gacattgccg gttccctgag cgatcagctc gcggtccgaa ttgtgccgcg
      361 ctcccgatag ggcatccatg acaacgcagg ttttgagagt gtcgatggaa agcagaatcg
      421 agagtgtcgt cgctggtacg atcacgaggc gcagcagatc gaaacgcaga tcgcccaggg
      481 accgcaggcg accggtcatc aggtcgagaa atgatccgct cagctgaatg ggtccgacga
      541 tcagagagtt cccttccgtc tgcagcagtg tccggtcgaa gagagccagc acgaaatacg
      601 ccagaatacc ggcaccaagc ccgagaaccg gtgcgggaat acgtttggtc aggcgctgac
      661 catggaccat gaccagcatc gcggtgaggc caaccagcac gctgggccat ttccagagac
      721 tgggggtgat gagcccttcg cccagcgggg ttcccctggg caaaccggtc agtttggcga
      781 gttgtccgag aatgatcagc aggccgaccg ccgaaagata cccggtcacg accggatacg
      841 gaatgaactt gatcaggcgc ccgcaaccga gcaagccgta aagtatttgc agcagcccgg
      901 cccccaggac gatcaaaccg agcagcaacg gaatggttgc cgcggtgatg ttcagagtgc
      961 cggccgaaag cagttcagcg gtgacggccg ccagaacggc cgctgccggc gcgcagggag
     1021 ctgaaatgag cacgccgttg cgacccacga ggggggcgac gatgccgatg gctgccgccc
     1081 caaggatgcc tgccatggca ccgtgagcca cgaagttcgc gcccagactg ctgtagatca
     1141 agaccccgaa ggcgatcgaa gccggcagcg cgaccagcat tgatgccaac ccaccccaga
     1201 ggtcacctgt cagagtggga acagaacgcg aagtcacgat tgatgaagtg gtactcacat
     1261 cctctcctgg ttcgatcaag gctgcaccga cagttcttct gatacggtga cacgacacga
     1321 taccagaatt ctgacagccg cgcacggcag ctcaccaggg cagcagcaaa gcagaataag
     1381 gcaggttttt gccctgacat tgccgccagg aggtggcgcg ggtgacggat taggcggact
     1441 gcggcaggct cggaggcgtc gacggggcga gggcggtttt ccaggcggcg ataaccttct
     1501 cgggcaggcg ggaagaggcg aacatctgtc gacagacgtc gagaaactcg gcgtccactc
     1561 gcgtcaacgg ctcgacctga ccggatggca gttcccattc gcgcggaaac acgatgccgc
     1621 gtgagcaact gtacaagctg agcttctgtc tgtcgtagac aagaacgcgg tccatgaagg
     1681 caagtctgcc gaggtgaagt gcgccgct
//
LOCUS       DAORHD010001182         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93160_length_1708_cov_3.437992, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001182 DAORHD010000000
VERSION     DAORHD010001182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93160_length_1708_cov_3.437992"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PKO06_19890"
     CDS             complement(<1..792)
                     /locus_tag="PKO06_19890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71977.1"
                     /translation="MNLCHTRFSRTFCWVLLLGTLTALWPAVSSAQSVSKMRDYLHLR
                     DALAERKSRIQTELTRAPEVGPVDARLDIRKVQQELVQVEDWLKKLEDNEEGLTGYYR
                     DRIARLISEIRYLLPALEKREASLAALQTTGTVIAVTPAPAPAQVAPIQASPTTSLAS
                     SSLAIASDAISELAPARVPPPPPELSKAERKKRRKAYFASMGVRLLRNATLKKPVAQP
                     TVTPIVAVTATATVPVAVKPTPATAPAPLQVPAVPTPVVAAGIDLT"
     gene            complement(807..1508)
                     /locus_tag="PKO06_19895"
     CDS             complement(807..1508)
                     /locus_tag="PKO06_19895"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HNV71978.1"
                     /translation="MTDKTRLESWWLGVVKALTKPLEALRNAFITGVIVAVPIAITVY
                     IAFLLFLFADGILGDAVADFMGYRIPGVGLILTILLFVLTGLVAQNVIGNRLVRWLDA
                     SLEMLPFVRPLYVGVKQVSDILFKKQQWDFQRVVMVEYPKSNSFALGFITGDFSRDLV
                     ERSPHQDSLVCVFVPTTPNPTSGFMLLVPKDKIYETRLSIEEAMKLIISGGLVKPGFE
                     HPPANTIFEDFTIPH"
     gene            complement(1518..>1708)
                     /locus_tag="PKO06_19900"
     CDS             complement(1518..>1708)
                     /locus_tag="PKO06_19900"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNV71979.1"
                     /translation="EDSETVGGFVLEKLGHIPRKDERVDEDQASFVVSEIKGNRILRV
                     QVTRREPLQATGLQAIEE"
BASE COUNT          353 a          478 c          533 g          344 t
ORIGIN      
        1 ggtgagatcg atgccggcgg cgaccaccgg tgtcggaacc gcgggaacct gcagcggcgc
       61 cggcgcggtt gccggagttg gtttcacggc caccggcacg gttgccgtcg ccgtcaccgc
      121 cacgattggg gtcacggtcg gctgagctac cggcttcttc agggttgcgt tgcggagcag
      181 cctgacaccc atcgatgcga aataggcttt gcgccgcttc ttgcgttcgg ctttgctcag
      241 ttcgggtggc ggcggcggaa cacgtgctgg agccagttcg ctgatcgcat cgctggcgat
      301 ggccagagag gaagacgcaa ggctggtcgt cggagaagcc tgaattggtg ccacctgcgc
      361 gggtgccggg gcgggagtca cggcgataac agtaccggtt gtctgcagcg ccgcaaggct
      421 tgcttcgcgt ttttccaatg cgggaagcag gtagcggatc tcgctgatca atcgcgcgat
      481 gcggtctcga tagtacccgg tcagcccttc ctcgttgtct tcaagcttct tcagccagtc
      541 ttcgacctga accagttcct gctgcacctt gcgaatgtcg agtctcgcat cgaccggtcc
      601 gacctcgggt gcacgggtca gctcggtctg aatgcgactc ttgcgttcgg ccagggcatc
      661 acgcagatgc aggtaatcgc gcattttcga gacgctctga gcgcttgata cggcaggcca
      721 cagagcggtg agagttccaa ggagcaaaac ccagcagaac gtgcgagaga agcgagtgtg
      781 acaaagattc atcggcagtt ccttgatcag tgaggaatcg tgaagtcctc gaaaatggtg
      841 ttggcgggcg gatgttcaaa gcctggcttg acgagaccac cagagatgat cagcttcatg
      901 gcctcctcga tgctcagccg ggtttcgtag atcttgtctt tgggtaccag gagcataaac
      961 ccggaagtag gattgggagt ggttgggaca aagacgcaga ctaaggaatc ctggtgtggg
     1021 ctgcgctcga ccagatcgcg ggaaaagtct ccggtaatga accccagcgc aaagctgttg
     1081 ctcttcggat actccaccat gacgacccgc tgaaagtccc attgctgctt cttgaagagg
     1141 atgtccgaga cctgtttgac gccgacatac agcggacgca cgaagggcag catttcgagc
     1201 gaggcgtcga gccagcgaac cagacggttc ccgatcacgt tctgggcgac caggccggtc
     1261 aggacaaaca gaaggatggt gagaatcaga ccaactcccg gaatgcgata gcccatgaag
     1321 tcggcgaccg cgtctccgag aatgccatcg gcaaacagaa acagcagaaa ggcgatgtag
     1381 acggtgatgg caatcggtac ggcaacgatg acgccggtga tgaaagcgtt ccgcagtgcc
     1441 tccagcggct tggtcagcgc tttgacgact cccagccacc aggattcgag acgggttttg
     1501 tcggtcatct tctgcgacta ctcctcaatc gcttgcaggc ctgtcgcctg caggggttca
     1561 cgtcgtgtga cctgcacccg cagaatacgg tttcccttga tctcagagac cacaaacgag
     1621 gcctgatcct cgtcgacacg ctcatccttg cggggaatgt gtccgagctt ctccaggaca
     1681 aacccaccga cggtttcaga gtcttcgt
//
LOCUS       DAORHD010001183         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93193_length_1708_cov_2.646098, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001183 DAORHD010000000
VERSION     DAORHD010001183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93193_length_1708_cov_2.646098"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..306
                     /locus_tag="PKO06_19905"
     CDS             55..306
                     /locus_tag="PKO06_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71980.1"
                     /translation="MNALWPGKQGEWTLKMILLIVIEGILLLTDWILPTEVPILPRIQ
                     AGFWEGLVIGFVVWVLWRLLCGFETSVETGKEPPAASGT"
     gene            451..>1708
                     /gene="pckA"
                     /locus_tag="PKO06_19910"
     CDS             451..>1708
                     /gene="pckA"
                     /locus_tag="PKO06_19910"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNV71981.1"
                     /translation="MKVKEWLSDKGFFSLDTIFQNLPSALLVEQALKRGEGVLADNGA
                     LVVRTGLRRGRSPNDKFVVETPDTKDVWWGSVNRPISVDVWNRIMNMGRSYLQNRDVF
                     VFDGYAGADPEYRLRVRVVSEKAWHALFSSTLFIRPPSSEQPNGDPDFTVINLCGMLI
                     PEFKSMGLSSGVFILVNFAERIILIGGTHYAGEIKKSIFSVMNYLLPKKGVFPMHCSA
                     NMGKNGDTALFFGLSGTGKTTLSADPERRLIGDDEHGWSEKGVFNFEGGCYAKCIKLS
                     RENEPQIYNAIRFGSVLENVVIDERRQPNYDDESITENTRATYPVTFIDNCIQEGVGG
                     HPKHVFFLAADAFGVLPPIARLTPEQAMYHFLSGYTAKLAGTEAGVMEPTATFSACFG
                     APFLVLHPFHYAEMLGQAMRKHGTTLW"
BASE COUNT          353 a          436 c          521 g          398 t
ORIGIN      
        1 tgggcggcgc ctggctggcc gccatgacgg ctgacctttc tggtggccgc ttcgttgaat
       61 gctctgtggc cgggaaaaca gggcgaatgg accctgaaga tgatcttgct gattgtgatc
      121 gagggaatcc tgctgctgac cgactggatt ctccccacgg aagtgccgat tctgcctcgt
      181 atccaagccg gtttctggga agggctggtg atcggtttcg tggtgtgggt gctgtggaga
      241 ctgctgtgtg gcttcgaaac atccgttgaa acgggaaaag aaccgcctgc ggcgtcgggg
      301 acctgatcag acgaacatca gcatacaggt ttcgcaatga cgaagagagg gaatgacctt
      361 ccttctcttt ttttcggggg tttgttcaac cgtcgattta ggagtaaagt aggagctaca
      421 gagaatggca tcaaggaaag gagtcgtatc gtgaaagtca aagaatggtt aagtgacaaa
      481 ggcttttttt cgctggatac catctttcag aacctgccct cggcgttgct ggttgagcag
      541 gctctgaagc gaggcgaggg tgttctggca gacaatggag cgctggtggt gcgcaccggc
      601 ttgcggcgtg gccgctcgcc gaatgacaag ttcgtggtcg aaacccccga cacaaaagac
      661 gtctggtggg gcagcgtcaa ccgtccgatc tcggttgatg tctggaatcg catcatgaat
      721 atggggcgca gttacctgca gaatcgtgac gtgttcgtgt ttgacgggta tgcgggcgct
      781 gatccggaat accggctgcg tgtgcgggtg gtcagtgaga aggcatggca tgctctgttt
      841 tcctcgaccc tgttcattcg cccaccaagt tccgaacagc cgaacggaga tccggatttc
      901 acggtcatca acctgtgcgg catgctgatt cctgagttca agtcgatggg cttgtcttca
      961 ggtgtcttca ttctcgtgaa ttttgccgag cgcatcattc tgattggtgg tacgcactat
     1021 gccggggaga tcaagaagag catcttctcg gtcatgaact acctgctgcc gaagaagggc
     1081 gtgtttccga tgcactgttc ggccaacatg ggcaagaatg gcgatacggc cctgtttttc
     1141 ggcctttccg gcacgggaaa aacgacgctt tccgcagacc ccgagcgccg gctgatcggc
     1201 gatgacgaac acggctggag cgaaaagggc gttttcaatt tcgagggtgg ctgttatgcg
     1261 aaatgcatca agctgagtcg tgaaaacgag ccgcagatct acaatgcgat tcgcttcggc
     1321 tccgtgctcg agaatgtggt catcgatgaa cgccgtcaac cgaactatga tgacgagagc
     1381 attaccgaga acacccgggc gacgtacccg gtgactttca ttgacaactg catccaggaa
     1441 ggggttggcg gacacccgaa acacgtcttt ttcctggcgg ctgacgcatt tggcgtgtta
     1501 ccgccgattg cccgtctgac ccccgaacag gcgatgtatc acttcctgtc agggtatacc
     1561 gcgaagctgg ctggtactga agccggagtc atggaaccga ctgccacctt ctccgcctgc
     1621 tttggcgctc cgttcctggt gctgcatccg ttccactatg ctgagatgct gggccaggcc
     1681 atgcgcaaac acggcacgac actctggc
//
LOCUS       DAORHD010001184         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93310_length_1706_cov_4.210781, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001184 DAORHD010000000
VERSION     DAORHD010001184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93310_length_1706_cov_4.210781"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="PKO06_19915"
     CDS             complement(<1..1669)
                     /locus_tag="PKO06_19915"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV71982.1"
                     /translation="MFSQLGVAISASLLASLLLAGTLAPLMAARLATARAIEKESRIH
                     HGYRRLLSSALRHPRLVMLACAFLFGATILLILPRLGIEFLPVPDDGMIGSPFRLPTG
                     MSLDESNQVIRPLAAYLGSDSRVLSCFHRVGFPAGAEEAGAVIGLSDHNEGEFFLRLK
                     PKSQRDVSTKDFIEEIRAVTRRIPGLEINLRQSGEQLFNADRAAITIQVFGPDLPTLK
                     TQLERVRESIRTVSGIRDLDFDLQGGRPELQIHIDRHKAMQAGLSLPDIAFALRTAVQ
                     GRRVSLFRQDGDEMYIRLRGPLQLEHDPEAVLDIPLEGAPATRSEHTASEGNNTRGVA
                     TLRSFAHLESGSGEEKLYRERQMRRGMVFANYAGRSLGEVVADIQQRLADLELPEGYF
                     LHIGGMYEDTVEAGRQLLYVFVLGVILVYMIMASQFESFKDPFVVFFSVPLAAIGMLL
                     GLYVFRQTINISSALGLVILLGIAVNNAIVLIDFIRQLRLQGVSLEQALVDGPTARLR
                     PVAMTTLTTVLGLLPLGFGYGDGAELQQPLAISLICGLSFSTLLTLFV"
BASE COUNT          399 a          478 c          535 g          294 t
ORIGIN      
        1 cgacaaacag cgtcagcagg gtcgaaaaag acagcccgca gatcagtgaa atggccagtg
       61 gctgttgcag ttcagcgccg tcaccgtatc cgaagcccaa ggggagcaag cccaggacag
      121 tcgtcagggt ggtcatggcc accggacgca gacgcgcggt tggaccgtcg acgagagcct
      181 gttcgagtga aacgccttgc agtcgcagtt gtcgtatgaa atcgatcagg acaatcgcat
      241 tgttcaccgc gatacctaac aggatgacga ggcccagagc ggaactgata ttgatcgtct
      301 ggcgaaacac atacaacccg agaagcatgc cgatcgcggc gagtggcaca gagaaaaaga
      361 cgacaaatgg atccttgaag gactcgaact gcgaggccat aatcatgtag acgagaatca
      421 cgcccagcac aaaaacgtag agcagttgac ggccggcctc gaccgtgtct tcgtacatgc
      481 cgccgatgtg cagaaagtag ccttcgggta gctcgagatc ggccaaacgc tgttgaatgt
      541 cggccaccac ttcgcccaga gatctcccgg catagttggc gaaaaccatg ccccggcgca
      601 tctgacgctc acgatacagt ttttcctcgc cgcttcccga ttcgagatgg gcgaatgaac
      661 gcagagtggc aacgcctcgc gtgttgtttc cctcggaggc ggtgtgctcc gaacgtgtgg
      721 caggcgctcc ttcgagcgga atgtcgagga cggcttcggg gtcgtgttca agctgcagcg
      781 gcccgcgcag gcggatatac atctcgtccc catcttgccg aaacaagctc acgcgccgcc
      841 cctgaacggc cgtgcgcaga gcgaaggcaa tgtcaggcag gctcaagccg gcttgcatgg
      901 ctttgtgacg atcgatgtgg atctgcaact ccgggcgccc gccctgcaga tcgaagtcga
      961 gatctcgaat gccgctcacc gtgcgaatag actcacggac tcgttcgagc tgagtcttga
     1021 gcgtcggcag atcagggccg aagacctgaa tggtaatggc cgcccgatct gcgttgaaga
     1081 gttgttcgcc actctgacgc agattgatct cgagcccggg aatgcggcgg gtgaccgcgc
     1141 ggatttcctc aatgaagtct ttggtggaaa cgtcgcgttg agacttgggt ttgaggcgca
     1201 gaaaaaattc gccttcattg tggtccgaaa gcccgatgac ggccccggct tcttcggctc
     1261 cggccggaaa accgacgcga tgaaaacagg aaagtactcg tgagtcactg ccgagataag
     1321 cagcgagagg gcgtatgacc tggttgcttt catcgaggga catgccggtc ggcaaacgga
     1381 aaggactgcc gatcatcccg tcatcgggca cgggcagaaa ctcgatgccg agtcgcggga
     1441 gaatcaggag aatcgtggca ccgaacagaa acgcgcaggc gagcatgacc agtcgtgggt
     1501 gccgcagggc ggaactcaga aggcgccggt acccatggtg gatgcgtgac tccttctcga
     1561 ttgcccgggc ggtcgccagg cgcgcggcca tcaacggagc cagggtcccg gcaagcagga
     1621 gcgaagccag cagtgaagcc gagatcgcta ccccgagctg ggaaaacatt ctggcggcga
     1681 tccccaccga aaacaggaag ggaatg
//
LOCUS       DAORHD010001185         1706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93316_length_1706_cov_3.875833, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001185 DAORHD010000000
VERSION     DAORHD010001185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93316_length_1706_cov_3.875833"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="PKO06_19920"
     CDS             <1..662
                     /locus_tag="PKO06_19920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71983.1"
                     /translation="AMVWDILKTPPTRTSVIMSPPSYASSGEAEATEIPLKQWVEGLE
                     PMLGKPPVQIQSVEVGTMNLAAAWANMEPSKREALLAGIVKTHALVVVATASASMGNV
                     SIMVLRDPAKAPVFIETLEEMAQNNLKQLQGTQSSMQVSDISVSDFPTELASPSRRFS
                     FTIGTTGESTKQKQVIYRLSIGSLLVEIFDGNVGIPDKDVVEITRRIRQAYEKHLGTR
                     K"
     gene            664..1191
                     /locus_tag="PKO06_19925"
     CDS             664..1191
                     /locus_tag="PKO06_19925"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV71984.1"
                     /translation="MIRLRDACVDDAMGIATVQVTGWQTTYQGIVPDSHLSKLTAEGR
                     ADMWRRGMSEPRPDQHWIVAETEAGRIIGFIASGRATEPVTDHAGEFYAIYLLPEHRG
                     QGVGTRLLSDSAQRLIRDGLSAALVWVLAANPWRRFYETRGGHYLCEKTITIGGTHLQ
                     EVAYGFRLSSLVGVA"
     gene            1188..>1706
                     /locus_tag="PKO06_19930"
     CDS             1188..>1706
                     /locus_tag="PKO06_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV71985.1"
                     /translation="MKPANKTAKQDPDSVLTPQDIIDRLGLKPHPEGGYYAETWRSSE
                     FIAQQHLAPRYSGNRRFGTAIYYFLTSDTFSALHRIESDEIFHFYAGYTVELFQLTET
                     GVGAVHRLGNDLCAGALPQLVVPRGIWQGCRLVQSGSWALLGCTVAPGFEYDDFCLAD
                     RHELLERFPQWRD"
BASE COUNT          356 a          513 c          492 g          345 t
ORIGIN      
        1 ccgccatggt ctgggatatt ctgaaaaccc ctcccacgcg caccagcgtc atcatgagcc
       61 ctccgtccta tgcatcgagc ggcgaagccg aggcaaccga gattccgctc aaacagtggg
      121 ttgagggact tgagccgatg ctgggcaaac ctccagtaca gatccagagc gtcgaggtcg
      181 gcacgatgaa tcttgcggcg gcctgggcca acatggaacc gtcgaaacga gaggcactgc
      241 tggccggcat cgtcaagacg cacgctctcg tcgtcgtcgc cacggcctca gccagcatgg
      301 ggaacgtttc gatcatggtg ctccgcgatc cggccaaggc tcccgtgttt atcgagactc
      361 tcgaggaaat ggcgcagaac aacctgaagc aactgcaggg aactcagtcc tccatgcagg
      421 tttccgacat ctcggtctcg gactttccaa ctgagctcgc aagcccgagt cgccgtttct
      481 cgttcaccat cgggaccaca ggcgaaagta cgaaacagaa acaggtcatc tatcgtctct
      541 cgatcggcag tctgctcgtc gaaatcttcg atggcaacgt cggcattccg gacaaggacg
      601 tcgtcgagat cacccgacgc attcgacagg cttatgaaaa gcatctcggc accagaaagt
      661 agcgtgatcc gcctgcgcga cgcgtgcgtc gacgacgcga tgggtatcgc gacagtacaa
      721 gtgaccggct ggcaaaccac ctaccaggga attgttccag attcgcatct ttcgaaactc
      781 accgcggagg ggcgtgcaga catgtggcga cgcggaatgt ccgagccgcg tccggatcag
      841 cactggatcg tcgccgaaac cgaggccggt cggatcatcg gattcatcgc ctcggggcgg
      901 gcaacggaac cggtcaccga tcatgcggga gagttctacg ccatctatct gctccccgag
      961 caccgcggac agggcgttgg cacccgactt ctctctgatt ccgcacagcg tctgattcgc
     1021 gatggcttgt cggcggcgct ggtgtgggtg ctggccgcga acccctggcg acggttctac
     1081 gagacccgcg gcggacacta tttgtgtgag aagaccatca cgatcggtgg aacccatctt
     1141 caggaagtcg cctacggctt tcgtctttcc tctctggtgg gggtggcatg aaaccagcga
     1201 acaaaaccgc caaacaagac ccagacagcg tgctcacccc gcaggacatt attgatcggc
     1261 tcggattgaa accgcatccc gaggggggat actacgcgga aacctggcga tcgtccgagt
     1321 tcatcgcaca gcaacatctg gcgccaagat attctgggaa caggcgattt ggtaccgcta
     1381 tctattactt tctcacttcc gacacttttt ccgcgctgca tcggatcgaa tcagatgaga
     1441 tctttcattt ctacgccggt tataccgtcg agctgtttca actgactgaa acgggagttg
     1501 gcgcagtgca ccggctgggc aacgacctgt gcgctggcgc tttgccccag ttggttgttc
     1561 cccgcggtat ctggcagggc tgccgcttag tccagagcgg ttcctgggca ttgctcggtt
     1621 gtaccgttgc tcccgggttc gagtatgacg acttttgtct ggcagaccga cacgaactgc
     1681 tcgaacgctt cccgcagtgg cgagac
//
LOCUS       DAORHD010001186         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93414_length_1705_cov_3.074545, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001186 DAORHD010000000
VERSION     DAORHD010001186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93414_length_1705_cov_3.074545"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKO06_19935"
     CDS             <1..571
                     /locus_tag="PKO06_19935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71986.1"
                     /translation="PDGGWNCDEAVYVKSAPKSSIVSTVPVLEAMLYCLRDGPNATEW
                     AAIDRGADYLVRRRLYRRESDPARVIDQNWLQVRFPRFYEYDFLRGAAWLADWARLRG
                     RTLPADLLAEVQERLLACTDRSTNVPVVGCCDLLRNSNYAPDSEGVWKVVKPPTSFSL
                     LEAVSRPGTSIPALHASGERVGAACAGSR"
     gene            complement(607..1125)
                     /locus_tag="PKO06_19940"
     CDS             complement(607..1125)
                     /locus_tag="PKO06_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71987.1"
                     /translation="MLQRFIFSWLIMCGLLSAPILEAAPIIIQQGTLIRIKLEQTIST
                     KTNRTGDMISFATSENLAIGKKVVVKKGAFVQAQLEKVRGPGRFGKNGSLKIRYLFVK
                     AANGRQVPITLGERSAKTNESMGFAAGASAAGYMILGPVGLLGGAFVRGQHITIPAGT
                     ELMVEVARDTVY"
     gene            complement(1130..>1705)
                     /locus_tag="PKO06_19945"
     CDS             complement(1130..>1705)
                     /locus_tag="PKO06_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71988.1"
                     /translation="EPIIEVKKAEIQEWNSGRDFRVSYLVKCGWLTSEAQDQLMVWIH
                     QKEDAYRHVALPRDTWFTMADLKLAVSKRVFNTQLGRVDAKAKPFFASLDEAREFIKK
                     KYGLVSITRDEVRYYVPGKLPRTDGAPYLCWWATIDDAANRGRQGHLNLITGTDESYE
                     NAIRHYAEPDNDASPVPTRATPTAIPHRKGE"
BASE COUNT          324 a          496 c          504 g          381 t
ORIGIN      
        1 gcctgatggc ggctggaact gcgatgaagc cgtctatgtc aaatcggctc ccaaaagctc
       61 gattgtctcg acagtaccgg tactcgaggc aatgctgtac tgcctgcgcg acggcccgaa
      121 cgcgaccgag tgggcggcga tcgaccgcgg ggccgattat ctggtgcgtc gtcgtctgta
      181 tcgccgagag tctgaccctg cgcgtgtcat cgaccaaaac tggttgcagg tgcggtttcc
      241 gagattctat gagtatgact ttctgcgtgg cgctgcctgg ttggcagact gggcgcggct
      301 tcgtggccga acgctccctg ccgatctgtt ggccgaagtg caggaacgac tgctcgcttg
      361 cacggaccgt tcgacaaatg taccggtagt tgggtgctgt gacttgcttc ggaacagtaa
      421 ctatgcgcca gactctgaag gagtctggaa agtcgtcaag ccgccaacga gtttttcgct
      481 cctcgaagcc gtcagtcgac cgggaacttc aataccggct ttgcacgcgt cgggcgagcg
      541 agtcggtgcc gcctgcgcgg gctcacgctg actgtgaagt cggccagcga gagctggcga
      601 tgactctcag taaaccgtgt cgcgcgctac ctcgaccatc agttcggtgc cggccggaat
      661 cgtgatgtgc tggccgcgca caaaggctcc gccgagcaga ccgaccggtc cgagaatcat
      721 gtaacccgcg gcagaggctc ccgcggcgaa gcccatcgac tcattcgttt tggctgagcg
      781 ttcaccgaga gtgattggaa cctgtctgcc gttcgccgcc ttcacgaaga gatagcgaat
      841 cttcaggctg ccgttcttgc caaaccgccc cgggccacgt actttttcga gctgagcctg
      901 gacaaacgcg cctttcttga cgacaacctt tttgccgatc gccaggtttt cactcgtcgc
      961 gaacgagatc atgtcgcctg tgcgattggt cttggtggaa atcgtctgtt cgagtttgat
     1021 gcgaatcaac gttccctgtt gaatgatgat cggcgccgct tccagaatcg gggcggacaa
     1081 caagccgcac atgatgagcc aactgaagat gaatctctga agcatgttct tactctccct
     1141 tccgatgcgg tatcgcggtc ggagtcgccc gggtgggaac cggtgaggca tcgttgtccg
     1201 gctcagcata atggcgaatc gcattctcat agctttcatc ggtgccggtg atcaggttca
     1261 gatgcccttg acgaccacga tttgccgcgt catcgatggt ggcccaccag cacagatacg
     1321 gagcgccgtc ggtgcgggga agcttcccgg gtacgtagta acgcacctcg tcacgagtga
     1381 tgctcaccag accatacttc ttcttgatga attcacgggc ttcgtcgaga ctggcgaaga
     1441 agggtttggc tttcgcatcg acgcgcccca gttgcgtgtt gaagacccgc ttgctgaccg
     1501 cgagcttcag gtcagccatg gtaaaccagg tatcgcgcgg cagggccacg tggcgatacg
     1561 cgtcttcctt ctggtgaatc cagaccatga gctgatcttg cgcttcgctg gtcagccagc
     1621 cgcatttaac cagatacgac acgcggaagt cgcgacccga gttccattcc tgaatctcgg
     1681 ccttcttcac ctcaatgatc ggttc
//
LOCUS       DAORHD010001187         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93432_length_1705_cov_2.720000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001187 DAORHD010000000
VERSION     DAORHD010001187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93432_length_1705_cov_2.720000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PKO06_19950"
     CDS             <1..183
                     /locus_tag="PKO06_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007312692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNV71989.1"
                     /translation="DILVEFAPQHTPGFLTLTALENELARVFKRRVDLRTPQEISRYF
                     RSEVLSGAATQYVAEV"
     gene            184..375
                     /locus_tag="PKO06_19955"
     CDS             184..375
                     /locus_tag="PKO06_19955"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HNV71990.1"
                     /translation="MSKAFTQARMPQIQWGLIIGMRNRLIHAYFDIDHQVVWQTVTID
                     LPPLIEHLQQFIADIEPDV"
     gene            complement(426..1217)
                     /locus_tag="PKO06_19960"
     CDS             complement(426..1217)
                     /locus_tag="PKO06_19960"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNV71991.1"
                     /translation="MSSSESYRQPPVVLHLPHSAVEIPPDVREKILLTDNELNKEILC
                     MTDLYTDEIFALPEQQAVSIRYPVSRLVLDPERFLDDAHEEMAARGMGLVYTRTSRGE
                     NLRYPPGASEREELIRRFYHPHHDALRHAVERALSDHLNCLLIDCHSFPSRPLPYEAD
                     QRPDRPQICIGTDPFHTPYDLEQSAKKQFQACGWEVAINRPFAGALVPTPFFRQDYSV
                     RAIMVEVNRGLYMNEATGAKLATFSHVAATIQQTIHLLIAEFPFT"
     gene            complement(1344..>1705)
                     /locus_tag="PKO06_19965"
     CDS             complement(1344..>1705)
                     /locus_tag="PKO06_19965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71992.1"
                     /translation="KGDSTMWLITTIGFFSVVQKPSSDVLTVRARVKKDLDTLREKYL
                     PNLSTTITNTGSDYPYRATVRHVDFAEALSAIAKDIDYGNFKSHVAQAMGLRRAHLYH
                     KIWRDLKELDHLEQLST"
BASE COUNT          380 a          463 c          474 g          388 t
ORIGIN      
        1 gatatcctgg tggaattcgc cccccagcat actcccggct tcttaaccct gacagcactc
       61 gaaaacgagc ttgctcgcgt cttcaagcgt cgtgtcgatc tccgcacacc ccaggagatc
      121 agtcgctact tccgcagtga agtcttgagc ggcgctgcaa cgcaatatgt cgcagaagtc
      181 tgactgagta aggcttttac acaggcgaga atgccgcaaa tccagtgggg attgatcatc
      241 ggcatgagaa atcgactcat tcacgcctat ttcgacatcg atcatcaggt ggtctggcaa
      301 accgtcacga tcgacctgcc gccgctgatt gagcatctcc agcagttcat tgcagatatc
      361 gaacccgatg tgtaacccag cttcgtattt caccacttcg aagcacgatt ccgaaactcg
      421 gggaatcagg taaacgggaa ctcggcgatc aggaggtgaa tcgtctgttg aatggtggca
      481 gcgacatgtg aaaacgtcgc gagttttgcg cctgtggctt cgttcatgta aaggccgcgg
      541 ttcacctcga ccatgatggc gcgaaccgag tagtcctggc ggaaaaacgg tgtcggcacc
      601 aaggccccag cgaatggccg attgatcgcg acctcccagc cgcaggcctg aaattgtttt
      661 ttggccgact gttcaagatc atacggcgtg tgaaaggggt cggtgccaat gcagatctgg
      721 ggtcgatcag gtcgctgatc agcttcatac ggcagtgggc gcgaggggaa actatgacag
      781 tcgatcagca ggcagttcag atgatctgag agggctcgtt caaccgcgtg ccgcaacgca
      841 tcgtggtgcg gatgatagaa gcgtctgatc agttcctccc gctctgatgc tccaggggga
      901 tatcgcagat tctcgcccct cgaagttcgt gtgtacacaa gccccatgcc tcgcgcggcc
      961 atttcctcgt gagcgtcgtc gagaaaccgc tcggggtcga gcaccagccg gctgaccgga
     1021 taccggatcg acaccgcctg ctgttcaggc agagcaaaga tctcgtctgt gtacaagtcg
     1081 gtcatgcaaa gaatctcttt gttgagctcg ttatcggtaa gcagaatttt ctctcgcaca
     1141 tccggcggaa tctcgactgc actgtgcggc aagtgaagca cgaccggcgg ctgtctgtaa
     1201 gattcacttg aagacatcgt cgtcctcctt ttcagattct gcgacatcgc ttcgttccta
     1261 catattccgg ataaatgttc gatgcgcgga aaaccgaccg atatgacaac cgcgtcatcg
     1321 taatcgagcg aaagcacagg attctatgtc gacaattgtt cgaggtggtc gagctctttc
     1381 aggtcgcgcc agatcttgtg gtagagatgg gcgcgacgaa gccccatcgc ttgcgccacg
     1441 tggctcttga aattgccgta gtcgatgtct ttggcgatgg cagacagcgc ctcagcaaaa
     1501 tcaacgtgcc tgaccgtggc gcgataaggg tagtcagacc cagtgttggt aatcgtcgtc
     1561 gagagattgg gcaggtactt ttcgcgcagg gtgtcgagat ctttcttgac gcgcgcccgc
     1621 accgtcagca catctgacga aggcttttgc accacgctga agaaaccgat cgtcgtgata
     1681 agccacatgg ttgaatctcc ttttc
//
LOCUS       DAORHD010001188         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93439_length_1705_cov_2.638788, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001188 DAORHD010000000
VERSION     DAORHD010001188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93439_length_1705_cov_2.638788"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /gene="pheT"
                     /locus_tag="PKO06_19970"
     CDS             complement(<1..1698)
                     /gene="pheT"
                     /locus_tag="PKO06_19970"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNV71993.1"
                     /translation="MLSHLGVARELSAIFQIPLKVSALDDAASDGETGKVTSVTMDAP
                     ALCPRYGAKVIEGVQVGPSPRWLKERLEKIGIRSINNVVDVTNYILMDIGHPMHAFDF
                     DTLEGRRIVVRSARAGEKFRTLDNVERTLDPSMLVIADADRPVALAGVMGGLETEVTE
                     KTRTLLLEAAYFDPVCIRRTAKALGMMSESSYRFERGTNIENIPIALNLATKHILAVA
                     GGKARSGIVDGYPEPRPQRTILVRRRRVEQILGISLTTAQIEALLNRLLMDVKRNGED
                     VLVAVPPFRHDLEQEADIIEEIARIHGYQHIPTTLPAISGVVMPPDPVTDLGDKIRDY
                     LIGQGFCQALNYSFVPAKVPELFHEGLPIRLVNPLSEDQAVLRTSLVWGMFDAIVRNV
                     NQDEYDIKMFEIGRVFHRDGGEGTDERVRLCLGICGRWNPGDWRHINEMVDTFHLKGV
                     LEALGRKLGRGFEFKASQREYYHPTDQMEIRCQNQLMGHGGRVHPKLLDNPKIPRQMY
                     LVEIDLTALARIPASMHTMKPIPGFPAIRRDLALVLPPEVTAGQVKTVIKEECGTLLE
                     "
BASE COUNT          342 a          537 c          470 g          356 t
ORIGIN      
        1 ctcgagcagt gtgccgcact cttccttgat caccgtcttc acctgaccgg ccgtcacctc
       61 gggcggcagg accaacgcca gatcacgtcg aatggcgggg aatcccggaa tcggcttcat
      121 cgtatgcatc gaagccggaa tgcgcgccag cgccgtcaga tcgatttcga ccagatacat
      181 ctgacgggga atcttcggat tatcgagcag tttcgggtgc acccgtccac catgtcccat
      241 cagctggttc tggcatctga tctccatctg atcagtcggg tggtagtact ctcgctgact
      301 cgccttgaac tcgaaacctc ggcccagttt gcggcccagt gcttccagaa cgcccttcag
      361 atggaaggtg tcgaccattt cgttgatgtg tcgccagtct cccgggttcc agcgaccgca
      421 gatacccagg cacagacgca cgcgctcatc ggttccctca ccgccgtccc ggtgaaagac
      481 gcgaccgatc tcgaacatct tgatgtcata ctcgtcctga ttcacgttgc ggacgatggc
      541 gtcgaacatg ccccagacca ggctggtacg caaaaccgcc tgatcttcgg agagcgggtt
      601 gactagacga attggcagcc cctcgtggaa gagctcggga actttggccg gaacgaagct
      661 gtagttgagc gcctgacaga acccctgccc gatcaggtag tcgcgaatct tgtcgcccag
      721 atcggtcacc ggatcaggcg gcatcacgac gccggaaata gccggcagcg tcgtgggaat
      781 atgttgatac ccatgaatgc gagcgatttc ctcgatgata tccgcttcct gttccaggtc
      841 gtgtcggaac ggcggcaccg cgaccagcac atcttcgccg tttcgtttca catccatcag
      901 caatcggttc agcagagctt cgatctgcgc cgttgtcagc gagatgccga gaatctgctc
      961 gaccctgcgg cgccgaacca gaatggtgcg ctgtgggcgc ggttcgggat atccgtcaac
     1021 gataccactg cgtgccttgc cgcccgctac cgcgagaatg tgcttggttg ccagattcag
     1081 cgcaatcgga atgttctcga tattcgtccc gcgctcgaac cggtatgaag attcggacat
     1141 catgcccagc gccttcgcgg ttcggcgaat gcacaccgga tcaaagtaag cggcttcaag
     1201 caacaaggtg cgggtttttt ccgtaacctc ggtctccaga ccgcccatga ctccggccag
     1261 cgccacgggg cgatcggcat cggcaatcac cagcatgctc ggatcgagag tcctttccac
     1321 attgtcgagt gttcggaact tctcgcccgc tcgagcgctg cgcacgacga tgcgacgccc
     1381 ctcgagcgta tcgaagtcga aggcatgcat cggatgcccg atatccatca gaatgtagtt
     1441 ggtcacgtcg accacgttat tgatcgatcg aattccgatc ttctccaggc gctccttgag
     1501 ccagcgaggg ctcggaccaa cctgaactcc ttcaataacc ttcgcgccgt atcgtgggca
     1561 aagagccggg gcatccatgg tcaccgaggt gacctttcct gtttccccgt ctgacgccgc
     1621 gtcatccagt gccgagacct tgagcggaat ctgaaagatc gccgacagtt cgcgcgccac
     1681 gcccagatgc gacagcacgt caccc
//
LOCUS       DAORHD010001189         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93607_length_1703_cov_3.346481, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001189 DAORHD010000000
VERSION     DAORHD010001189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93607_length_1703_cov_3.346481"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PKO06_19975"
     CDS             <1..1076
                     /locus_tag="PKO06_19975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV71994.1"
                     /translation="RIAGETSAAQIQTQPSGIVVFFTHPQRPEAFMLAIRAAESLNQA
                     FAKRTEMPFSPRLMLHCQETLHGALDDENQTIGYFAETAFDHKAIAQVQDSDEIIFSE
                     EMAYLMQDVTELEEIEVGTAEARLRAFIFRGFKVVETLATAFPAATPWSRMLSLKVLK
                     NHGDLAIADRLIQWYESADSSIRYHILDTLDRLRPPGICDFVVKIVQDESDPKVLSKA
                     IAVLGKVGNEQHVALLADKLRTSDRRVKANVVEALESIGGKKVYEFLNLVVDEPDNRV
                     KANILIALGKYGDMKVFDLLNRMIKDSDKNMRASAAYALGKLGMVQGVDPLIAALSDK
                     DITVRRQVVASLTSLRADLDIDA"
     gene            1250..>1703
                     /locus_tag="PKO06_19980"
     CDS             1250..>1703
                     /locus_tag="PKO06_19980"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71995.1"
                     /translation="MTFDEAYKKGNEALKARRFDEAKACFEEALRAKPNDIYTINKLA
                     MISKEKGDFNEALNLLEQSLKIKEKDLYTNYLIGNAYLEQGNVDKAIETLENTVKIDP
                     KDKYARNSLALAYRMQGNYEKCVETLNQALRYSPDDLYNKLSLATVRFE"
BASE COUNT          408 a          527 c          447 g          321 t
ORIGIN      
        1 gccgtatcgc cggcgagacc agcgccgccc agattcagac tcaaccctcc ggaatcgtcg
       61 tcttcttcac gcacccgcag cgccccgaag ccttcatgtt ggccattcgg gcggctgaat
      121 ccctgaatca ggcgttcgcc aagcgcaccg aaatgccgtt ctcgccgcgc ctcatgctgc
      181 actgccagga aacactgcac ggcgcgctcg atgatgaaaa ccagaccatc ggctacttcg
      241 ccgaaaccgc gttcgaccac aaggctatcg cgcaggtgca ggacagcgac gaaatcatct
      301 tcagcgagga aatggcctac ttgatgcagg acgtgaccga actcgaggag atcgaggtcg
      361 gtaccgccga agcgcggctg cgggccttca ttttccgcgg gttcaaagtt gtcgaaaccc
      421 tcgccaccgc gtttcctgct gccaccccct ggagccggat gttgtcgctc aaggtgctga
      481 agaatcatgg cgatctcgcc atcgctgatc gcctgattca gtggtatgag agcgcagaca
      541 gcagcattcg ctatcacatc ctcgacacgc tcgatcggtt gcgcccccct ggcatctgtg
      601 atttcgtcgt caagatcgtc caggatgaat ccgacccgaa ggtgctctcc aaggccatcg
      661 ccgtactcgg aaaagtcgga aacgaacagc acgtcgccct gttggccgac aagcttcgta
      721 ccagcgaccg acgtgtgaaa gccaatgttg ttgaggctct tgagtctatc ggcggcaaaa
      781 aggtgtacga attcctcaac ctggtggtcg acgaacccga caaccgggtc aaggcgaaca
      841 ttctcatcgc gctcggcaaa tacggcgaca tgaaggtctt cgacctcttg aaccgcatga
      901 tcaaagattc cgacaagaat atgcgcgctt cggccgccta tgcgcttggc aagcttggca
      961 tggtccaggg cgtcgatccg ctgatcgccg ccctgtcgga caaagacatc accgtgcgac
     1021 ggcaggtcgt cgcctcactg acctcgctgc gcgctgatct cgatatcgac gcctgaccct
     1081 caccggtttc gacctcgacc cttcggtctc gtcaccgcgc tcgtttccga caaaaaatcc
     1141 tgcgcgagac gcttgatgca gcaggcgaaa aagcgtaatc tgatgagagt acagtgccat
     1201 gccggcccgc attccagagc ccggcatatt gagacttggg agtcccatca tgacatttga
     1261 tgaagcctac aagaagggca acgaagccct caaggcccgg cgattcgacg aggccaaagc
     1321 gtgctttgaa gaagccctgc gcgcgaaacc gaacgacatt tacacgatca acaagcttgc
     1381 catgatctcg aaagagaagg gcgatttcaa cgaagcgctc aatctgctcg agcagtcgct
     1441 gaaaatcaag gaaaaagacc tctacacaaa ctacctcatc ggcaacgcct atcttgaaca
     1501 gggcaacgtg gacaaagcca tcgagacgct cgaaaacacc gtcaagatcg accccaaaga
     1561 caagtatgcc cgcaactcgc tcgcgctcgc ctatcgcatg caagggaact atgagaagtg
     1621 tgttgagacc ctgaatcagg cgttgcgata ctcccctgac gatttgtaca acaagctcag
     1681 cctggcaacc gtgcgtttcg aac
//
LOCUS       DAORHD010001190         1703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93654_length_1703_cov_2.081311, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001190 DAORHD010000000
VERSION     DAORHD010001190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93654_length_1703_cov_2.081311"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..>1703)
                     /locus_tag="PKO06_19985"
     CDS             complement(57..>1703)
                     /locus_tag="PKO06_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71996.1"
                     /translation="ASLVIESSFAANIKEDKFEQILATVKAIGTHCYVFVENGKTVSD
                     KAIENVQKTFDEKIYPTDTTYFGSEWKPGIDGDERITLLLLDIRDGFDPQSNPGYVAG
                     YFFAGDEFLQSQIPADVPVKSNEREMFYLDIHPSNPEEENYMGVVAHEFQHMIHFAQD
                     PQEFTWVNEGCSMMAMQFCGYRDSVLNHIAMFMKTPDNSMPSWSDEQTMACYGEVYLW
                     NYYLMHRYLGEDEATRQKFFRELVENKKQGGASYDEALKPFKTDFTTAFQDFCVTNFI
                     NSPKMEQGQFAYDKSLGRLRLPASTQIKAVPGKAQEKVFPWSGEAIKVDLTGAKTTVK
                     VEFGGPNVKVTEKDTLKFTAFAVFSDSRDAQVPMLKKIETSVSPDKKLQVGAVEVSTS
                     GKYDTLNVIVTLTCTTAIPDQAFKQVKDGIPYIVQVSDSGIMTASGNTRRIPIRPMID
                     QYVPAAATLVTAEGAASEQALTTLEGLTHDITREVKGQLEAGSLSAVKELIEAASDAQ
                     KREALRPLAKKVADQVSASKVQSQSQNVELESMIKQLRDF"
BASE COUNT          337 a          474 c          475 g          417 t
ORIGIN      
        1 aaaacaacag ggggcaccta ggttcggtgc cccctgctga aagcagatgg tttcgcttag
       61 aagtctcgca gctgcttgat catactctcg agttcgacgt tctgactctg gctctgcacc
      121 ttgctcgcgg agacctgatc ggccactttc ttcgccagcg ggcgcagtgc ttcacgcttt
      181 tgagcatcag aagcggcttc gatcaattct ttcacggcac tcagcgagcc ggcttcgagc
      241 tgccccttca cttcgcgggt aatgtcgtgg gtcagccctt cgagggtcgt cagggcctgt
      301 tcggaagcgg caccctcggc ggtgaccagc gtcgcggcgg ccggaacata ctggtcgatc
      361 atcgggcgaa tcgggatccg gcgagtgttt ccagaggcag tcatgatgcc gctgtcgctg
      421 acttgtacga tgtagggaat accatctttg acctgtttga acgcctgatc aggaatcgcg
      481 gtcgtgcagg tgagcgtcac gatcacgttc agggtgtcat atttcccgct ggtcgatact
      541 tcgacggcac caacctgcag ctttttgtcg ggtgagacac tggtttcgat cttcttcagc
      601 atcggcacct gagcatcgcg agagtcagag aacaccgcga aggccgtaaa cttcagcgta
      661 tccttttccg tgaccttcac gttcgggccg ccgaattcga ctttcaccgt ggtcttggca
      721 ccggtcaggt caaccttgat cgcttcgccg ctccagggaa acaccttttc ctgggctttg
      781 ccgggcacgg ctttgatctg ggtcgaggca ggcagacgca ggcgacccag agatttgtcg
      841 taggcaaact gcccctgttc catcttcggg ctattgatga agttggtgac gcagaaatcc
      901 tggaacgcgg tggtgaaatc ggtcttgaac ggcttcagcg cttcgtcgta ggaagcgccg
      961 ccctgcttct tgttttccac cagctcgcgg aagaacttct gccgggtcgc ttcgtcttcg
     1021 cccagatagc gatgcatgag atagtaattc cagaggtaaa cttcgccgta gcaggccatc
     1081 gtctgttcat cagaccatga aggcatgctg ttgtcggggg tcttcatgaa catggcaatg
     1141 tgattcagta ccgaatcacg atacccgcag aactgcatag ccatcatcga gcagccttcg
     1201 ttcacccagg tgaattcctg cgggtcctgc gcgaaatgaa tcatgtgctg aaactcgtga
     1261 gcgacgacgc ccatgtagtt ctcttcttcg ggattcgacg gatgaatgtc gagatagaac
     1321 atttcgcgct cattggactt gactggaaca tcggccggaa tctggctctg caggaactcg
     1381 tcacccgcga agaaataccc ggcgacatag ccagggttac tctgcggatc aaatccgtcg
     1441 cgaatgtcga gcagcagcag ggtgatgcgt tcgtcaccgt cgataccggg tttccattcg
     1501 ctaccgaaat aggtcgtatc ggtggggtag atcttctcat cgaaggtctt ctgcacattc
     1561 tcgatcgcct tgtcagagac cgtcttgcca ttctcgacaa atacgtagca gtgggttccg
     1621 atggccttca ccgtggctag aatctgttcg aacttgtctt ctttgatgtt cgccgcgaag
     1681 ctcgactcga taaccaggct cgc
//
LOCUS       DAORHD010001191         1702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93689_length_1702_cov_3.333333, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001191 DAORHD010000000
VERSION     DAORHD010001191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93689_length_1702_cov_3.333333"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKO06_19990"
     CDS             <1..216
                     /locus_tag="PKO06_19990"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV71997.1"
                     /translation="ALWYKKAIETDPDYDLAHLNLAVSLIELGELETARIHAEKAAQL
                     DPGTHLAQVDELMKKIQVAKIKKDEEM"
     gene            complement(243..770)
                     /locus_tag="PKO06_19995"
     CDS             complement(243..770)
                     /locus_tag="PKO06_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71998.1"
                     /translation="MEKRRYSIQDVQDITKLSPTALQEFITRFRKYFSIETEESPDGP
                     KVYLDQISLERLMFIKNLETNQSQSKEEALCQIRTSKGRSGVPERKSLAGILVNFLDS
                     LTVEIKNIENNMNQILHRYVQVLKDLNQCRSENRQMRKEMDQLKKRQEKILLQLREQD
                     DEAILEQKPRRDQIN"
     gene            929..>1702
                     /locus_tag="PKO06_20000"
     CDS             929..>1702
                     /locus_tag="PKO06_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV71999.1"
                     /translation="MSGHSTIDDILRNLTVFLKGQIPSLQAKHTVFEISQQVQSIIMN
                     LRTSITQLEGAGHFDPRATPEYKAMARELDTMKAAHQKLEESLFLEMNKSERIHQERS
                     KDSGVVQEARERMAKADEQIHALETEVVELRAQLARAQSETAKPRPPSGIEPGIKAAY
                     DRKVAEVERQRDQLLQTLHEKDAEIMRLNSSLHDMDVSLREGAARADDLRREIKHLTD
                     QREGLVARLTTLEGGLNTERSDFMRIRQGLEQQIELAGKA"
BASE COUNT          402 a          458 c          462 g          380 t
ORIGIN      
        1 gccctctggt acaagaaagc catcgaaacc gatcccgatt acgatctggc gcaccttaac
       61 ctggcggtca gtctgattga actcggcgag ctcgaaacgg cacgtattca cgccgagaag
      121 gccgcgcaac tcgatccggg cacgcatctc gcccaggtag acgaattgat gaaaaagatt
      181 caggtggcca agatcaagaa ggacgaggaa atgtaaaaag gcgcccgaag gcgccttttg
      241 tcctagttaa tttggtctct tcttggcttt tgttcaagta ttgcttcatc atcctgctcg
      301 cgtaactgca gcaggatttt ttcttgtcgc ttcttgagct ggtccatttc cttgcgcatc
      361 tgtcgatttt cggaccggca ttgattgaga tccttgagca cctgcacata gcgatgcaga
      421 atctggttca tgttgttctc gatgttcttg atctcgaccg taagagaatc gaggaaattg
      481 acgagaatgc cggccagact cttccgctca ggaacaccag accgaccttt cgaagtacga
      541 atctggcaca gcgcttcttc tttcgactga ctctggttgg tctcgagatt cttgatgaac
      601 atcaggcgct cgagtgagat ctgatccaga tacactttcg gaccatcggg actttcttcc
      661 gtttcgatcg agaagtattt gcggaatcgg gtgatgaatt cctgcagtgc ggtgggagaa
      721 agcttggtga tgtcctgaac gtcctggatc gaatagcgcc gcttttccat gaggatctcc
      781 ttgagaaagt gaacagataa cctatcgata ggcggcacga gttcgttgag cttttttttc
      841 tcgcgatttc gaagccctga ggcttgaaat acgtgcgctc tcagttgtat cttcatggca
      901 cactacactt cgccgcagga agaggaccgt gtccggacat tccacgatcg atgatattct
      961 tcgcaatctg accgtgtttt tgaagggtca gataccttct ctgcaggcca aacataccgt
     1021 cttcgagatc tcacagcagg tacaatcgat catcatgaac ctgcgtacgt cgatcacgca
     1081 gctcgagggt gcggggcact ttgacccacg ggccaccccc gaatacaaag ccatggcccg
     1141 cgagctcgac accatgaagg cggcgcacca gaagctcgaa gagtctctct tcctcgagat
     1201 gaacaagagc gaacggattc accaggaacg cagcaaggat tcgggcgtcg tgcaggaagc
     1261 ccgtgagcgc atggcgaagg cggacgaaca gatacacgcc ctggaaacgg aagtcgtcga
     1321 gttgcgagca cagctcgccc gagcgcagtc cgagaccgca aaaccccgcc caccatccgg
     1381 aatcgaaccc ggcatcaagg ccgcctatga tcgcaaggtg gcggaagtcg aacggcaacg
     1441 cgaccagctg ctgcagactc tccacgagaa agatgccgag atcatgcggt tgaattccag
     1501 tcttcatgac atggatgtct ctctgcgaga gggagctgcg cgggcagacg atctgcgccg
     1561 tgaaatcaag catcttaccg atcagcgaga gggtctggtt gcgcgattga ccaccctcga
     1621 aggcggtctc aacactgagc gttctgactt catgcggatt cgtcaggggc tcgaacagca
     1681 gatcgagctg gccggcaagg cc
//
LOCUS       DAORHD010001192         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93792_length_1701_cov_3.023086, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001192 DAORHD010000000
VERSION     DAORHD010001192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93792_length_1701_cov_3.023086"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="ftsZ"
                     /locus_tag="PKO06_20005"
     CDS             complement(<1..679)
                     /gene="ftsZ"
                     /locus_tag="PKO06_20005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNV72000.1"
                     /translation="MFDFNQAVVNGAEIKVIGVGGGGTNAVNRMIASNVVGVEFISAN
                     TDAQALQSSNAKVKIQIGDKLTKGLGAGANYEIGKKAAEEDRERLVEALDGADMVFIT
                     AGMGGGTGTGAAPVVAEVAREVGALTVAVVTRPFKFEGKRRAAAAELGIKNLRERVDA
                     IIVIPNDKLLEVSKPNVTMMEAFQYADDVLRQGVQGISDIIVREGMINTDFADVRTVM
                     TQSGSALM"
     gene            complement(734..>1701)
                     /gene="ftsA"
                     /locus_tag="PKO06_20010"
     CDS             complement(734..>1701)
                     /gene="ftsA"
                     /locus_tag="PKO06_20010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNV72001.1"
                     /translation="QGIVAIKGNSREITDEDVERAVENAKTAVSIPTHQQIIHTLPRE
                     FVVDEQKEISNPVGMVGSRLEAQVHIISGAITAVQNIMKSCEMANLKIHEVVLQPYAS
                     SLAVLSEDEMKLGVVLIDIGGGTSDLMIFQNGHVVHSFCAPVGGHYVTNDIAVGLKTG
                     TQNAEALKIKYGVALTANADSNQQIEVSCPGGEKTKKPVTQKDLAEIIEPRMEEMFNE
                     LKEQIIKHVSPEMIPAGVVLTGGTALMNGCPELAEEILGLSVRRGYPQNVTGLKEKIN
                     SPVYSTAAGLIKFGMKYYPTHVPEAGILGGLGDRFRGLLRKFFGT"
BASE COUNT          361 a          508 c          437 g          395 t
ORIGIN      
        1 ccatcaaggc agatccgctc tgggtcatga cggtgcgcac atcggcgaag tcggtgttaa
       61 tcatgccttc gcgcacgatg atgtcggaga tcccctgcac gccctgacgc aacacgtcat
      121 cggcatactg gaacgcttcc atcatggtga cattcggctt ggacacttcg agcagcttgt
      181 cattgggaat aacgatgatg gcatcgaccc gttcgcgcag gttcttgata ccgagctcgg
      241 cagcggctgc acggcgcttg ccttcaaatt tgaacggacg agtgacgacg gcaacggtga
      301 gtgcgcccac ttcgcgagcg acttccgcga caaccggagc ggcaccagta ccagtgccac
      361 cacccatacc cgcggtgatg aaaaccatgt ccgcgccatc gagtgcttca acgaggcgct
      421 cacgatcttc ttcagcggct ttcttgccaa tctcataatt ggcgccggcg cccaagccct
      481 tggtcagctt gtcgccaatc tgaatcttca ctttggcatt ggaactctgc aatgcctgag
      541 catcggtatt ggccgaaatg aactcgaccc ccaccacgtt cgatgcgatc atgcggttga
      601 ccgcgttggt gccaccaccg ccgacgccga taaccttgat ctcagcccca ttcaccactg
      661 cctgattaaa atcgaacacc taaggcctcc ttttccttac tgcccactcc gggaaagaaa
      721 ttaaaactct gtgttacgta ccaaagaact tgcggagcaa cccgcggaac cgatcgccca
      781 gaccgccaag aatgccggct tcgggaacat gagtcgggta gtacttcatg ccgaacttga
      841 tcaacccggc tgcggtcgaa tagacaggac tattgatctt ctctttcaac cccgtcacat
      901 tttgtggata cccgcgccgg accgaaagac cgaggatctc ctctgccagt tcggggcacc
      961 cgttcatcag ggcggtgccg cccgtcagaa ccacgccggc cgggatcatt tccggggata
     1021 cgtgtttgat gatctgttct tttaactcgt taaacatctc ctccatgcga ggttcgatga
     1081 tctccgcgag atctttctgg gtcacgggct tcttggtttt ttcgccgccc ggacacgaaa
     1141 cttcaatctg ttgattggaa tcagcgttcg cggtcagggc aacgccgtat ttgatcttca
     1201 atgcttcagc attctgcgtg ccggttttca ggccgaccgc gatatcattc gtgacgtagt
     1261 gtccgccgac cggtgcacag aacgagtgaa cgacgtggcc attctggaaa atcatgagat
     1321 ccgaagtgcc cccgccaatg tcaatcagca cgacaccgag tttcatctcg tcttcggaga
     1381 gcactgccag actcgaagca tagggctgca gtacaacttc atgaatcttg agattcgcca
     1441 tctcacagga tttcatgatg ttctgaaccg cggtaatggc accggaaatg atgtgcacct
     1501 gcgcttcgag gcgactaccc accatgccga ccggattgct gatctctttc tgttcgtcga
     1561 cgacgaactc gcggggaagc gtatgaatga tctgctgatg cgtcggaatc gatacggcgg
     1621 ttttcgcatt ctccacggcg cgttcgacgt cttcgtcggt aatctcgcgc gagtttccct
     1681 tgattgcgac gataccctgg c
//
LOCUS       DAORHD010001193         1701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93819_length_1701_cov_2.589915, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001193 DAORHD010000000
VERSION     DAORHD010001193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93819_length_1701_cov_2.589915"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKO06_20015"
     CDS             complement(<1..285)
                     /locus_tag="PKO06_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72002.1"
                     /translation="MTCFVGHHRGLGLLFLLFAVCLIASPWLLATETLDLFAHQGRLT
                     FFLGSLSASGIILLLVFGRFRHLRLPLLSPALLLGSIIGVTTVLSPFLVSL"
     gene            complement(282..779)
                     /locus_tag="PKO06_20020"
     CDS             complement(282..779)
                     /locus_tag="PKO06_20020"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV72003.1"
                     /translation="MFLSAFFLLLSLPFSAVAPSPPPFSPTHAGQRLPHHEARLGTHP
                     LRIMLARSLEEKMQGLMYFTHLDQDEGMLFVYPQTQRMAFWMKNTLIPLDLVFFNERL
                     QVTEWIEGMQPGGQKPDHALPRYTSRGPARYALELSEGSVRRLGIQSGQQLDIPLVLL
                     FSGEP"
     gene            complement(811..>1701)
                     /locus_tag="PKO06_20025"
     CDS             complement(811..>1701)
                     /locus_tag="PKO06_20025"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV72004.1"
                     /translation="KVLTNKADGIGLYRTEFTYLIRRRFPTEDELIEIYTSVMSRLGN
                     SEVVIRTIDLGGDKISHLVNQPAEKNPELGWRAVRLSLDRLDIFRTQLRAILRTAATI
                     GRQQAKILFPMISNLGELRRSQAFLHEVCDELKKEGVAIPTRILIGAMIEVPSAAILA
                     DKIAREVDFLSIGSNDLVQYTLAVDRTNSKVAHLYQPANPAVLRLLHQVIQAAEKNGK
                     PLSICGELAGDSRYTLMLLGLGLRELSMNSVFIPRVKQVVRLISIEEARKAVLPLLEL
                     DTAEEIERELAQLNSRLGIN"
BASE COUNT          387 a          466 c          531 g          317 t
ORIGIN      
        1 gagactgacc agaaaaggcg agaggacggt ggtgacgccg atgatgctgc cgagcagcag
       61 agccggcgag agcaggggca ggcgcaggtg gcgaaagcgt ccgaatacca gcagcaggat
      121 gatgccggat gcgctcagcg agccgaggaa aaaggtcagt cgcccctgat gtgcgaacag
      181 gtcaagcgtc tctgttgcca gaagccaggg cgaggcgatc aggcagacgg cgaacagaag
      241 aaacaacaga ccgagaccgc gatgatggcc gacaaaacac gtcatggctc accggaaaag
      301 agcagcacca gggggatatc gagctgctgg ccgctctgaa tcccgagccg tctgacactc
      361 ccctcgctga gctcgagagc atatctggcc ggacctcgac tggtatacct cggcaatgcg
      421 tgatcgggtt tctggccgcc tggctgcatg ccctcgatcc attcagtgac ctgcagacgt
      481 tcgttgaaga ataccaggtc gagcggaatc agggtgtttt tcatccagaa agccatacgc
      541 tgggtctgcg gatacacgaa cagcatcccc tcatcctggt ccagatgggt gaaatacatc
      601 aggccctgca tcttctcttc caggctgcgg gcgagcatga tgcgcagggg atgtgttccc
      661 agacgagcct cgtggtgggg taaccgctgc ccggcgtgtg tcggtgaaaa ggggggaggg
      721 gagggggcga ccgcggagaa gggcagagaa agcaacagaa agaaggccga caaaaacatg
      781 cttactccgc gaaccggccg ggcaccctca tcagttgatg cccagacgtg aattgagctg
      841 tgccagctcc ctctcgatct cttcggcggt atccagttcg agaagcggca gcaccgcttt
      901 gcgtgcttcc tctatggaga tcaggcgcac gacctgcttg acgcgcggaa tgaagacgga
      961 attcatcgac agctcgcgca gtccgagacc cagaagcatc aaggtatatc ggctgtcgcc
     1021 ggccagttcg ccgcagatgc tgagaggttt tccgttcttc tcggcggcct ggatcacctg
     1081 atgcaacaat cgcagaaccg cgggattggc tggttgatac agatgcgcga ctttggaatt
     1141 ggtacgatcg acggcaaggg tgtactgcac gaggtcgtta ctgccgatcg agagaaagtc
     1201 gacttcgcgc gcgatcttat cggccagaat ggcggcagat ggtacctcga tcatggcacc
     1261 gatgagaatc cgcgtgggaa tggcgacacc ctctttcttg agttcgtcgc acacctcatg
     1321 cagaaaagcc tgcgagcgtc ggagttcgcc cagattcgag atcatgggaa acagaatctt
     1381 cgcctgctgc cgcccgatcg tcgcggcggt gcgcaggatc gcccgcagct gggtacggaa
     1441 gatgtcgaga cgatcaagag acagacgcac ggcccgccag ccgagctcgg ggttcttctc
     1501 ggccggttga ttgaccagat gcgagatctt gtcacccccc agatcgatgg ttctgatcac
     1561 tacttcagaa tttcctaacc tggacatgac ggaggtatag atctcaatca gttcgtcttc
     1621 cgtgggaaag cgtcgtcgaa tcagataagt gaactcggtg cggtacaggc cgatgccatc
     1681 tgccttgttg gtcagcacct t
//
LOCUS       DAORHD010001194         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_93873_length_1700_cov_3.474772, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001194 DAORHD010000000
VERSION     DAORHD010001194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93873_length_1700_cov_3.474772"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..>1700)
                     /locus_tag="PKO06_20030"
     CDS             complement(162..>1700)
                     /locus_tag="PKO06_20030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72005.1"
                     /translation="PDDNMNRTPVLTNTLPASISLPLLLVMVLLCVKPAMASVFKFDG
                     KYHAKSTIDSTRKDYAEVLYPDYTGVGVTSPLAYDKTFVAYVSDMQLKLKGDLTPTQY
                     LDFAETLHYQFYEPQDYKAYSLDSYKYRYVDHYFNMTYGIMLGTADAFKFDFINNIYR
                     IPIDNYWDFTSNLGKVRFNHKVNVNTSLAFEAQYEERDYPNDASSDYQEGAVLVDFST
                     FLPEVLRYRPVGNSTRGEREPFQKTPTGMTTRKAVDYYTTWTKKPGQSEPEAKYLADV
                     VRGDLALTLIGDLRSRNRTTLNNAYYQPSGILRGVYDASDKLKINFENTYYNRSYDRE
                     SDAYFLYDYSSNRFYLSGTHRPDTRFTYIMSWTNEFYDHDRVKEQDYHLNIFQLETYY
                     TYGRSNASLVLKDTLTRYKYPRLYYADADLVQIIFGYDYPITKRFLFHFKDEWVDNDQ
                     KNNEDLLYSSYVRNTWRVALEKILSQVYSLEVGYQNKRERHEVFTANNITEKTLFFSL
                     LSKY"
BASE COUNT          389 a          405 c          464 g          442 t
ORIGIN      
        1 tgccccaaca acagatcgcc gccggcgagc aacagatcgg gtttttcgac tgccgacacc
       61 ggcgtggtca gctgagtcag ttcgcgcaga ctcagttcat gcaggattct gccgaggcaa
      121 agaattttca tgcggatgtt ttccctcaga tgttggtctg ttcagtattt cgacaacaga
      181 ctaaaaaaca gggttttctc ggtaatgttg ttggcggtga aaacctcgtg ccgttcacgc
      241 ttgttctgat agccgacctc gagactgtaa acctgactca gaatcttttc gagcgcgacc
      301 cgccaggtat tgcgcacata tgaggaatac agcagatctt cgttgttttt ctggtcatta
      361 tccacccact cgtccttgaa atggaagaga aagcgcttgg taatcggata atcataccca
      421 aaaatgatct gaaccaggtc ggcatcagca taatagaggc gcggatactt gtatcgtgtc
      481 aacgtatcct tcaacaccag gctggcattt gatctgccgt aggtgtagta ggtttccaat
      541 tgaaagatgt tcagatgata atcctgctct tttacccgat catggtcata gaactcgttg
      601 gtccacgaca tgatgtaggt aaagcgcgtg tccgggcgat gcgtgcccga aagatagaat
      661 cgatttgatg agtagtcata caggaagtag gcatcggact ctcgatcgta gctgcggttg
      721 taatacgtat tctcaaagtt gattttcagc ttgtcggaag cgtcgtagac tccacgcagg
      781 attcccgagg gctgatagta ggcgttgttc agagtagtgc gattgcgact gcgcaggtcg
      841 ccgatcagcg tcagagccag atctcctcgc accacgtcgg ccaggtattt tgcttcaggc
      901 tcactctggc cgggtttctt tgtccaggtt gtataatagt caaccgcttt tctcgtcgtc
      961 attcccgtgg gtgttttctg aaagggttcg cgttcgccac gcgtcgagtt gcccaccggg
     1021 cgataccgga gcacctcggg caggaaggtt gaaaagtcaa ccagcaccgc cccttcctga
     1081 tagtcggaag aggcgtcgtt gggataatcg cgctcttcat actgggcctc gaaggcaaga
     1141 ctcgtgttga cgttcacctt gtgattgaaa cgtaccttgc cgagattcga cgtgaagtcc
     1201 cagtagttgt cgattggtat gcgatagatg ttgttgatga aatcgaactt gaacgcgtct
     1261 gcggtgccca gcatgatgcc gtaggtcatg ttgaagtagt gatcaacgta acgatatttg
     1321 tacgagtcga gagagtatgc tttgtagtcc tgaggctcat aaaactgata gtgaagtgtc
     1381 tcggcaaaat cgagatactg tgtcggtgtc agatcgccct tgagtttgag ctgcatgtcg
     1441 cttacatagg cgacaaacgt cttgtcatag gccagcgggg aggtgacgcc cacgcccgtg
     1501 taatccggat acagaacctc agcatagtct tttctggtgc tgtcgatggt cgatttggcg
     1561 tggtacttgc catcgaactt gaacaccgag gccatcgccg gcttgacgca cagaagcacc
     1621 atgaccagca gcagcggcag gctgatactg gccggaagag tgttcgtcaa taccggtgtt
     1681 ctgttcatgt tgtcatcagg
//
LOCUS       DAORHD010001195         1698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94083_length_1698_cov_3.041388, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001195 DAORHD010000000
VERSION     DAORHD010001195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94083_length_1698_cov_3.041388"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKO06_20035"
     CDS             <1..887
                     /locus_tag="PKO06_20035"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72006.1"
                     /translation="LGRIYEERLNDYPRAAEMYGKIVQNHPTFLSIDKVVYGLANCQE
                     KTGNVEDAVKWYQEIVVKYPYSSFFKTAQSRMKSLAAGTQHAKGALEAQEGVIENART
                     DAQAAKATMDLAAMLTSQGEYKKAVEEYRKIANDPSNPELARQAYTRMASLLDEKEKD
                     YKGAAAVLEEMVSKFPNEQGTDKSLYKLGRIHEENLQTLRTREQGDGSTLYKKSNENV
                     RKAIEYYDRLTESFPDADVSADAYLRKGELYEGRIKDSDEALRQYKEYLRRFPDHDQA
                     ETIRKKVKKLESGESDDE"
     gene            859..1584
                     /locus_tag="PKO06_20040"
     CDS             859..1584
                     /locus_tag="PKO06_20040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72007.1"
                     /translation="MNPEKATTSSTQVRHTLRIWGLVFTLLCGTFSAAVAADATVIGL
                     SGTMLVPGLEVMAPGTARGAFHFVGNEVEHEASLKGVFAYNDDTEIAVMKRFVTGRGK
                     DQYDPVFAAKFKVRPNLALAGVIDTTVGYKDSVMLLTGVPGNRVVLGVGANIAMEATE
                     RYAHFGRYEDPKSEVDPLFFIMGADIHVDDDTDVTLDYTGNDFVLGLRHRFNEVMAAD
                     FGYFTPDRLREESRYCLGLNFGF"
BASE COUNT          390 a          467 c          503 g          338 t
ORIGIN      
        1 atcttggccg catctacgag gagcgactca acgattatcc gcgcgcggcg gagatgtacg
       61 gaaagattgt tcagaatcac ccgaccttcc tttccatcga caaggtcgtc tacggcctgg
      121 cgaattgtca ggaaaaaact ggcaatgtgg aagatgcggt caagtggtat caggaaatcg
      181 tcgtgaagta tccgtacagc tcctttttca agactgctca gtctcgcatg aagagtctgg
      241 ctgccggtac gcaacatgcc aagggagcgc tcgaggcaca ggaaggggtg atcgaaaatg
      301 cccgtaccga cgcccaggcc gccaaagcga ccatggatct cgcggccatg ttgacctctc
      361 agggggaata caagaaggcg gtcgaagaat accgcaagat tgccaatgac ccgtcgaatc
      421 cggaactcgc tcgccaggcc tatacccgca tggcatcact gctcgatgaa aaggaaaagg
      481 actacaaggg tgccgcggcg gtgctcgagg aaatggtgag caagtttccc aacgagcagg
      541 gtaccgacaa gagtctctac aagctcggcc ggattcatga agagaatctg cagacgctgc
      601 gaacccgcga gcagggagac gggtcgacgc tgtacaagaa gtcgaacgag aacgtgcgca
      661 aagccatcga atactacgac aggctcacgg aaagtttccc ggatgccgat gtatcagctg
      721 acgcctacct cagaaaaggg gagctgtacg aaggtcgcat caaggattcc gatgaggccc
      781 tcaggcagta caaggaatac cttcgccggt ttcccgatca tgatcaggcg gaaaccattc
      841 gcaagaaggt gaagaaactt gaatccggag aaagcgacga cgagtagcac tcaggttcga
      901 cacacgctgc ggatctgggg tctggttttc actctgctct gtggcacgtt ctctgccgca
      961 gtggccgccg atgccacggt catcggtctt tccggaacca tgctggtgcc cggactcgag
     1021 gtcatggcac ctggtacggc gcgtggggcc tttcactttg tcggcaatga agtcgagcac
     1081 gaggcctcgc tcaaaggtgt gttcgcctac aacgacgaca ccgaaatcgc tgtcatgaag
     1141 cgctttgtca ccggtcgcgg caaggaccag tacgacccgg tgtttgcggc aaagttcaag
     1201 gtgcgcccga atctggcgct ggccggggtc atcgacacca cggtcggata caaggattcc
     1261 gtcatgctcc tgaccggcgt gcccggcaat cgggttgtcc ttggtgtcgg ggcgaacata
     1321 gcgatggaag cgaccgagcg gtatgcgcac ttcggacgct acgaggatcc gaagtccgag
     1381 gtcgatccgt tgttcttcat catgggggcc gacattcacg tcgatgacga caccgacgtc
     1441 actctcgatt atacgggcaa cgactttgtt ctggggctgc gtcatcgatt caacgaagtc
     1501 atggccgccg acttcggcta tttcacgccc gaccgtctgc gcgaagagag ccgctactgc
     1561 cttgggctga acttcggttt ctgagccggc tatggggctc ttcctcgtca atctgctgct
     1621 ggtcggaata ctggtagctc tcgggtatgc gtatctcgat ctgcaggaac gttacacgcg
     1681 tcatgcaact cccgatct
//
LOCUS       DAORHD010001196         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94178_length_1697_cov_3.020097, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001196 DAORHD010000000
VERSION     DAORHD010001196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94178_length_1697_cov_3.020097"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /gene="gpmI"
                     /locus_tag="PKO06_20045"
     CDS             complement(<1..1538)
                     /gene="gpmI"
                     /locus_tag="PKO06_20045"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015405397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNV72008.1"
                     /translation="MSDRTLKPLSGMPTPKGPVVLVIMDGVGIGKKDESDGVHMAYTP
                     TLDQLMAEPIMTTLKAHGTAVGLPSDEDMGNSEVGHNALGAGRVFAQGAKLINEAIAS
                     GKIFTSRAWNSVKEKVAAGGTLHLIGMVSDGNVHSHIDQLYAILDRSVTEGFKKVRVH
                     GMLDGRDVGEKSGLEYFEPLEKKLASLSQGGRSYRIASGGGRMVTTMDRYNANWAVVE
                     KGWKAHVLGQGRGFASASEAISTYYRENPEITDQYMDSFVIVENGKPVGTIEDGDAVV
                     FFNFRGDRAIQISRAFEEQNFTEFNRQRHPKVFYAGMMQYDGDANIPANYLVEPPAID
                     GTLGQYLCASGVTSFAVSETQKYGHVTYFWNGNNSGYINEKLEKYVEIPSDRITFDLR
                     PWMKAAEITDTVIEAIQSGKYKFIRLNLANGDMVGHTGIPLSIRIAVETVDLSLKRLL
                     PVVQAAHGTLVVTADHGNADCMWTEKKGVRSPMVAHTLNPVPFIIKDFSGTNRYALSG
                     VEKPG"
BASE COUNT          318 a          497 c          482 g          400 t
ORIGIN      
        1 cccggtttct cgaccccact caacgcgtac cggttggtac cggaaaagtc tttgatgatg
       61 aacggaaccg ggttcaacgt gtgggcgacc atgggtgagc gcacgccctt cttctcagtc
      121 cacatgcagt cggcgtttcc gtgatctgcc gtgacaacca gtgtgccgtg tgccgcctgc
      181 acgactggca gcaggcgctt gagactgaga tcgaccgttt caaccgcaat gcgaatcgac
      241 agaggaatgc cggtgtgacc aaccatatcg ccgttggcga gattcaggcg aatgaacttg
      301 tacttgccac tctgaatggc ctcgatgacg gtgtcggtga tttccgctgc tttcatccag
      361 gggcggagat cgaaggtgat gcgatcagaa ggaatctcaa cgtatttttc gagcttctcg
      421 ttgatgtagc ccgagttgtt gccgttccag aaataggtga catggccgta cttctgcgtt
      481 tcggaaacgg cgaacgaagt cacgccgctg gcgcagagat actggccgag cgtgccgtca
      541 atggcaggcg gttcgaccag gtagttcgcg ggaatattgg catcgccgtc atactgcatc
      601 atcccggcgt agaacacctt gggatgccgc tggcgattga attcggtgaa attctgctct
      661 tcaaaggcgc gcgagatctg aatggcccga tcgcctcgaa aattgaaaaa cacgacggca
      721 tcaccgtctt cgatggttcc gaccggtttg ccgttttcaa cgatcacgaa actgtccatg
      781 tactgatcgg tgatctcggg gttttcgcgg taatacgtgc tgatcgcttc gctggccgac
      841 gcgaacccgc gcccctgtcc caatacgtgc gccttccagc ccttttcgac cacggcccag
      901 ttggcgttgt accggtccat ggtcgtgacc atgcggccgc cacccgaggc gatgcggtag
      961 ctgcgcccgc cctgcgacag gcttgccagc ttcttttcaa gtggttcgaa gtattcgaga
     1021 ccgctcttct cgccgacgtc gcgcccgtca agcatgccat gaactcgtac ttttttgaat
     1081 ccttcggtga ccgatcggtc gagaatggca tacagctggt cgatatggct gtgcacgttt
     1141 ccgtcggaaa ccatgccgat cagatgcagc gttccgccgg cggccacctt ctctttcacg
     1201 gagttccagg cgcggctggt gaagatcttc cccgacgcga tggcttcgtt gatcaacttg
     1261 gcgccttgag cgaagacgcg tccggcaccc agagcattat gtccgacttc cgagttgccc
     1321 atatcttcgt ctgacggcaa tccgacggct gttccgtgtg ctttcagagt ggtcatgatc
     1381 ggttccgcca tcagctgatc gagggttggc gtgtaagcca tgtgaacgcc gtcgctctcg
     1441 tctttcttgc cgatgccgac gccatccatg atcaccagaa cgacaggccc tttcggcgtg
     1501 ggcatgccgc tgaggggttt caaggttcta tcactcatgg gattcctccc ggtttttcag
     1561 agttttgcgc cgcgtattcc agcgccttcc ctgtatacca ccccatgccg cggcccgaca
     1621 agatactcgc gcagaacttc atggcgcgtc gccgagtgaa cgaggtgcgt tcactcgagt
     1681 gacgggtacg tgactcc
//
LOCUS       DAORHD010001197         1695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94399_length_1695_cov_2.719512, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001197 DAORHD010000000
VERSION     DAORHD010001197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94399_length_1695_cov_2.719512"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKO06_20050"
     CDS             <1..660
                     /locus_tag="PKO06_20050"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNV72009.1"
                     /translation="YAYSNFGYCLLGQVIEAVSGQTYEAYVRKHVLQPAGLSGFEVGK
                     TLAPAPREVAYYSAVGVGKAASVFDARPGETEWPYGGWCLENMDAHGGWTATAVDVLR
                     FVLALDGQKGHQRILSADSIKTMLARPPAPLFSGEPYWYAMGLLVRPMDRGVNWWHLG
                     SLDGTTTIMVRTFHGLAWVGLFNNRPQNYEEFNAALDVAFWKAVEGVTAWPTHNLFLG
                     Q"
     gene            763..1617
                     /locus_tag="PKO06_20055"
     CDS             763..1617
                     /locus_tag="PKO06_20055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV72010.1"
                     /translation="MMSTVKLLVALLVVLGVVIGCVWWYMAASMAPETAIEKRDLAWL
                     KQILTDTPERAHYRDLYGYTLMHTAAVVDFPEAIALLAEAGVSVNASCSLSDAELESL
                     KDLARDEGRRPDNVVWSYQETEGMTPLHFAVIFHSHGALRELMRLRADVNALTPRRES
                     PLFWAGRSWNAEAVPILVASGAEVNVQTLIGHKTPLHAVFDAFPEKPLLGLGDGFLWD
                     FAQKYYPLKSQATDSRRKVVKALLQAGVDHAKPNLDGKTAAELCPASEVTECFGSILS
                     AERPPDNE"
BASE COUNT          326 a          425 c          581 g          363 t
ORIGIN      
        1 tacgcgtatt ccaacttcgg gtattgtctt ttagggcagg tcattgaggc ggtctcgggg
       61 cagacctacg aggcgtacgt tcgaaaacac gttctgcagc ccgcgggatt gtccggtttc
      121 gaagttggaa aaacactcgc tccggcgccg agagaagtcg cgtattactc ggcagtgggc
      181 gtcggaaagg cggcgtcggt gtttgatgcc aggccgggag agaccgagtg gccgtatggg
      241 ggctggtgcc tggagaacat ggatgcccac ggcggatgga cggcgacggc cgtcgatgtg
      301 ctgcgttttg tcctggcgct cgatggacag aagggtcatc agagaattct cagcgcggac
      361 tcgatcaaga cgatgctggc tcgaccgccc gcgccgctgt tttccggcga accgtactgg
      421 tacgccatgg ggttgctggt gcggccgatg gaccgggggg tgaactggtg gcacctgggt
      481 tcgctcgatg gcacgacgac gatcatggta cgcacctttc atggtctggc ctgggtcggc
      541 ctgttcaata accgtccgca gaactatgag gagttcaatg cggcactgga tgtggctttc
      601 tggaaagcgg tggagggagt gaccgcctgg ccgacccaca acctgttcct cggacagtga
      661 ggttcctgtt tctgctcgaa gccctgaagc gcttgtgtgg gagcgaaatt ccccgatcat
      721 cgttgcggtg cgagttaccc gcggatggca tggcgaggaa agatgatgtc gacggttaaa
      781 cttctcgtgg ctcttctggt tgttctcggc gtggtgatag ggtgtgtctg gtggtacatg
      841 gcagcgtcca tggctcctga gacggccatc gagaaacgcg atcttgcctg gttgaagcag
      901 atactgaccg atacgccgga gcgggcgcac tatcgcgact tgtacggata cactctgatg
      961 cacacggccg cggtggtgga ttttccggaa gcgattgcgc tacttgcaga ggccggggtg
     1021 agcgtgaatg ccagctgctc gttgagcgac gcggagctgg agagtctcaa ggatctggcg
     1081 agagacgagg gacgccggcc ggataacgta gtgtggtcct atcaggagac agaaggaatg
     1141 acgcccctgc attttgccgt catctttcac tcgcacggtg ccttgcgaga actgatgcgg
     1201 ctgcgcgccg acgtaaacgc gctgacgccg cggcgcgaat cgccgctgtt ctgggcaggt
     1261 cgcagttgga acgccgaagc ggtgccgatt ctggtagctt cgggggcgga ggtcaacgtt
     1321 cagaccctca tcggtcacaa gacgccgttg cacgcggtct ttgacgcttt tccggaaaaa
     1381 ccgttgcttg gattgggtga tgggtttctc tgggacttcg cgcaaaagta ctatccgttg
     1441 aaatcgcagg caacggactc gagacgaaaa gtggtgaaag cacttctaca ggccggtgtc
     1501 gaccatgcca aaccgaatct cgatgggaag actgccgctg aactgtgtcc ggcaagcgaa
     1561 gtgacggagt gcttcggatc aatactttcc gcggagcgac cccctgacaa cgagtgagcg
     1621 tctgacgaaa gctgctgatg ggcgagggtt ggcggggaag caatttgtac taggtggtgc
     1681 tgggggattg tggtt
//
LOCUS       DAORHD010001198         1694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94453_length_1694_cov_3.915192, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001198 DAORHD010000000
VERSION     DAORHD010001198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94453_length_1694_cov_3.915192"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PKO06_20060"
     CDS             <1..1243
                     /locus_tag="PKO06_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72011.1"
                     /translation="YGDAEDKYTPGPDIPYLPGTLLSDEEYEKLHHRELWEHRNSLPH
                     ANEEGYYYNEEADAWVSSLQGHLDFFSRLDFGQSTADESDSDSEDNENSSNEEEGEQP
                     GDTPEYEVTQGQPPESPGNEEKPEDGQTQQGQKPNKPNKPGETTVTKPDPDKDFNEML
                     KKRQELLQKRDQLLALDKQLNKMLDGLRDQARAAADLAVAFSSKSYDDFKDFVKDYTS
                     GKAADFLKEKMIDKLGKYGKLVEKGLELKEKVEGAIETGERVNDLATGDTEAKLNAAK
                     DFLKDACPIGEEIDGILTGAFNVLHGYELTRHAEELGNMLNEEYKKKAWILEDAGKLH
                     GELGRLDEAIKNDPRYGGLSDDTKKQMNAEIEDSKFFRHGETANEYLQRGQRPSDEKV
                     GDRNPDAPAALKDLPAYIWTS"
     gene            1271..>1694
                     /locus_tag="PKO06_20065"
     CDS             1271..>1694
                     /locus_tag="PKO06_20065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72012.1"
                     /translation="MNKLLTFVVGILLLTTSLAMAAGSESTPPRDLETWRAASKEIYA
                     LLADGTVLNMQKKFVEATPLLRQALDKALESGIPSQIDRIRLELARALVETDQGAEAA
                     TLAETVYRRTPDSSMAALRYGQALLSVGRTHDAIGILKQ"
BASE COUNT          463 a          484 c          477 g          270 t
ORIGIN      
        1 ctatggggac gctgaagata agtatacccc gggtccagac attccctacc tgcccggaac
       61 cctcttgagc gacgaggagt acgaaaaact tcatcacagg gaactctggg aacatcgcaa
      121 tagtctgccg catgcgaacg aagagggcta ctactataac gaagaagctg acgcctgggt
      181 ttcaagcctt cagggccacc tggacttttt ctcgcgactc gacttcggac agtcaaccgc
      241 agatgaatct gattctgatt ccgaagacaa cgagaattcc tcgaacgaag aagagggcga
      301 gcagcctggc gacactccgg aatatgaagt gacccaggga cagcccccgg agtcaccggg
      361 gaacgaagag aagccagaag acggtcagac ccagcagggg cagaaaccca acaagccaaa
      421 caagccaggt gagacgaccg tcaccaagcc tgacccggac aaagacttca atgaaatgct
      481 gaagaagcgt caggagctgc tgcagaagcg tgatcagctg ctcgctctcg acaagcagtt
      541 gaacaagatg ctcgacggtc tgcgcgatca ggcgcgcgct gccgccgatc tggccgtagc
      601 gttttccagc aaaagttacg acgatttcaa agacttcgtg aaagactaca ccagcggcaa
      661 agcagcagat ttcctcaagg aaaagatgat cgacaagctc ggcaagtatg gcaagctcgt
      721 cgaaaaaggc ctggaactga aagagaaagt ggagggcgct atcgaaaccg gcgagcgcgt
      781 caacgatctg gcgacgggcg acaccgaagc gaagttgaac gccgccaagg actttctcaa
      841 agacgcgtgc ccgatcgggg aagagattga cggcattctc acgggggcgt tcaacgtgct
      901 gcacgggtat gagctcaccc gccatgccga agaactcggg aacatgttga acgaagaata
      961 caagaagaaa gcctggatcc tggaggatgc cggcaaactt catggcgaac tcggtcgact
     1021 cgacgaagcg atcaagaatg atccccgcta cggtgggctc tcagatgaca cgaagaaaca
     1081 aatgaatgcc gagatcgaag acagcaagtt cttccgccat ggtgagaccg ccaacgagta
     1141 tctgcaacga ggccagaggc cgagcgacga gaaagtcggt gaccgaaacc cggacgctcc
     1201 cgccgccctc aaagatctgc ccgcttacat ctggaccagc tgacgaagaa ccaacgaagg
     1261 aacagcaacc atgaacaaac ttctcacttt cgtcgtagga attctacttc tcaccacgag
     1321 tctggcgatg gcagcaggca gcgaatcgac gccgccgcga gacctcgaaa cctggcgcgc
     1381 ggcttccaaa gagatttatg cccttcttgc cgatggcacc gttctcaaca tgcagaagaa
     1441 gttcgtcgaa gcgactccgc tcctgcgtca ggcgctcgac aaggctctcg aaagcggcat
     1501 tccgtcacag atcgaccgca ttcgcctcga gttggcacgc gccctcgtcg agacagacca
     1561 gggcgctgaa gcggcaacgc tcgccgaaac cgtctaccgg cgaactcccg acagcagcat
     1621 ggccgcgctt cgctatggtc aggctctgtt gtcggtgggg cgaacccatg acgccatcgg
     1681 catcctgaag cagg
//
LOCUS       DAORHD010001199         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94577_length_1693_cov_3.308303, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001199 DAORHD010000000
VERSION     DAORHD010001199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94577_length_1693_cov_3.308303"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PKO06_20070"
     CDS             <1..1002
                     /locus_tag="PKO06_20070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72013.1"
                     /translation="VFRRFVLLSFFVVPSCAYGEGEEMIYNDNPGFRLTAKQIRGSQD
                     FDPPAGTTYRDYMSRILSGDPARQSEGYSSLMQPYNLRAGGAMAAPLSANDYQGCDGL
                     RIKAGAHFDQYREQMLNVRPVTDATTPVIGLQERISRYFPDSESFVLQARGVRTLTES
                     SRRFWVGGTVVVDQGLDLSAGIHEEDIRGGIIIVNGPLQLGEVIPAKVAAQLSVDHLT
                     DAAFSSKLAESFTKQTPEEMLTFVVLGGHPITLTGRKYAGIHLVSLSDGATPINLPAE
                     PFFFAGSLAVSRPEISRLTRQLARGSAFFFHPLFASERPPLTVAMETSMSDYDFSIQ"
     gene            1046..1501
                     /locus_tag="PKO06_20075"
     CDS             1046..1501
                     /locus_tag="PKO06_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72014.1"
                     /translation="MEILIGVLILGLAIIPIFGISSSSTRSAFLVGRHMMATQIAQSL
                     LEWFIAQSYQDARQEAQTLAGQVIPVTEDLVFRHILQAATPESRDQALGEFRRTFRQM
                     QYTVEHEERDEPQQGKVLLLKVTLYYRLAEDQSNTRRFMISTLKFPEDV"
     gene            1498..>1693
                     /locus_tag="PKO06_20080"
     CDS             1498..>1693
                     /locus_tag="PKO06_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72015.1"
                     /translation="MIEVFRPTRPIRDTRPCTLGGVRGEHRACLTRQRSGVTLTEVLI
                     AAFLGVTLMGLVILLMSRTNI"
BASE COUNT          333 a          493 c          489 g          378 t
ORIGIN      
        1 gtgtttcgcc ggttcgtgct gctgtcattc tttgtcgttc cgagctgcgc gtatggcgaa
       61 ggcgaggaaa tgatctataa cgacaacccc gggtttcgcc tgaccgcgaa acagattcgg
      121 ggctcgcagg atttcgatcc accggctgga acaacctatc gcgactatat gtcccgcatt
      181 ctctcgggtg atccggcgcg acagagcgag ggctattcga gtctgatgca accctacaat
      241 ctgcgtgccg gaggggcgat ggcggcgcct ctttccgcca acgattatca gggctgtgat
      301 gggctcagaa tcaaagccgg cgcacacttc gaccagtatc gcgagcagat gctgaacgtt
      361 cgacctgtca ccgatgccac gacgccggtg attggcttgc aggaacgtat cagccgctat
      421 tttcccgaca gcgaatcgtt cgtgctgcag gcgcgggggg tgagaaccct gaccgaaagc
      481 tcgcgacgat tctgggtggg cggtaccgtt gtcgtcgatc agggtcttga tctttctgcc
      541 ggcattcacg aggaagacat tcgcggggga atcatcatcg tgaatggtcc actgcagctg
      601 ggcgaggtga tacccgccaa ggtcgccgca cagctcagtg tggatcatct gacggatgcg
      661 gcgttttcca gcaagctggc cgagtcgttc acgaaacaga ctcccgaaga gatgctgacc
      721 ttcgtcgtgc tgggcggtca cccgatcacg ctcacgggcc gcaagtatgc cggcattcac
      781 ctggtcagtc tcagcgacgg agcaaccccg atcaatctgc cggctgaacc gtttttcttt
      841 gccggatcac tggcagtcag tcgccccgag atcagccggc tgacccgaca actggcccgc
      901 ggcagcgcat ttttctttca tcctctcttt gcctccgaac gaccaccgtt gaccgtagcc
      961 atggaaacct cgatgagtga ttatgatttc tcgattcaat aaccaacgta tgagagtacg
     1021 agtctcgcac actggcttct cggttttgga aatacttatc ggcgttctca ttctcggtct
     1081 ggcgatcatt ccgattttcg gcatttcgtc ttcgtcgacc cgatcggcct ttctcgtcgg
     1141 acggcacatg atggcaaccc agatcgccca gagtctgctc gaatggttca ttgcccagtc
     1201 gtatcaggat gcgcggcaag aggcgcaaac cctcgcgggc caggtgatac cggtcacgga
     1261 agatctggtc tttcgtcaca ttctgcaggc ggcaaccccc gagagccgcg atcaggcact
     1321 cggcgagttt cgtcgcacct ttcggcagat gcagtatacg gttgaacacg aagaacgcga
     1381 cgaaccgcag cagggcaagg tcctgttgtt gaaagttacc ttgtactacc ggctggcgga
     1441 ggatcagtcg aacacgcgcc ggtttatgat ctcgacattg aagtttcccg aggacgtatg
     1501 atcgaggtat tccgacccac tcgcccaatt cgtgacacac ggccctgcac tctgggtggc
     1561 gttcggggtg agcatcgggc gtgcctgacc cggcaacgat ccggcgtgac gctcaccgag
     1621 gtgctgatag cggccttcct gggggttacc ctgatgggcc tggtgattct actcatgtcg
     1681 cgcaccaata tca
//
LOCUS       DAORHD010001200         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94592_length_1693_cov_2.711233, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001200 DAORHD010000000
VERSION     DAORHD010001200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94592_length_1693_cov_2.711233"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKO06_20085"
     CDS             complement(<1..406)
                     /locus_tag="PKO06_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNV72016.1"
                     /translation="MHEHQLLLAFGLTLFAGLSTGIGSALAFFARRTNTRFLSLSLGF
                     SAGVMLYVSLIEIFPKARAALVQPFGEKQAYLYTTLAFFGGVILIALIDQLVPSIENP
                     HEMRMLEELTPTEEEVVKKSAQESDLLRMGMFS"
     gene            506..1669
                     /locus_tag="PKO06_20090"
     CDS             506..1669
                     /locus_tag="PKO06_20090"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNV72017.1"
                     /translation="MSVMLKTSGKLRILGMMSGTSGDAIDGTLVTFDERDSYKLDWYD
                     SYEFAAPVRQRLQSLMVGITGPDLARAEAYVAELYAEAVRSFRCRHREQIDAIAVHGQ
                     TLVHVPTPVEWDGMKVSGTIQALSPSWLAHRCGLPVVSDFRRRDMAAGGQGAPLVPYG
                     DARFFGHLSGNTVALNIGGIANITVLSGGPGAQVVSAFDTGPGNMLMDAAMVWATNGE
                     QCYDRDGAFAASAPYSEALLQELLQTPYLRTAPPKTTGREDFGCTRWEEIKKAWEKQM
                     SAAELVSTFCEFTARSIAEAIVTFVAPTWPPDRVVVAGGGAYNPELCRRIRERLPPCC
                     AFERSDVYGVPVMAREAMAFAALGEAFLRGRPANVPAATGARQPVVLGSYTPA"
BASE COUNT          343 a          449 c          556 g          345 t
ORIGIN      
        1 ctgagaacat gcccatccgt aacagatcgc tttcctgggc cgacttcttc accacttcct
       61 cttcggtcgg tgtcagttcc tcgagcatgc gcatttcgtg gggattctca atcgagggta
      121 ccagttggtc gatgagcgca atcaggatga ctcccccgaa gaacgccagg gttgtataca
      181 gatacgcctg cttttcaccg aacggctgca ccagcgcggc tcgtgctttg ggaaagattt
      241 cgatcagcga gacatacaac atgacgccgg cggaaaagcc gagggagagg gaaaggaacc
      301 gggtgttggt gcggcgggcg aagaaagcca gcgcactgcc gatgccggtc gagagtccgg
      361 cgaagagagt cagaccaaag gcgagaagaa gctgatgttc gtgcatatcg atcatatata
      421 ctggtttcgt gatgatacac aagacctgag gacaatctgg tagaatgccg cgcgacggca
      481 gtgtgcaacg gaaaggatgg gacagatgag cgtaatgctg aagacgtcgg ggaaacttcg
      541 gattctcggg atgatgagtg gaacctcggg agatgctatc gatggcacct tggtaacctt
      601 tgacgagcgg gactcataca aacttgactg gtacgatagt tacgagtttg ccgctccggt
      661 gaggcaacgt ctgcagtcgt tgatggtcgg cattaccggt cctgacctgg caagagctga
      721 agcctacgtg gcggaactgt atgcggaagc ggtccgatca ttccggtgtc gccatcgaga
      781 acagattgat gccatcgccg tgcatgggca gacactcgtt catgtgccca cgcccgtgga
      841 gtgggatgga atgaaagtct ccggtaccat tcaagcgttg agcccctcct ggttggcgca
      901 tcgctgcggg cttccggtcg tcagcgattt tcgtcgccgt gacatggcgg ccggtggaca
      961 gggtgcgccg ctggttccct acggtgacgc caggtttttc ggccatctgt cgggaaacac
     1021 ggtggcgttg aacattggtg gcattgcgaa catcacggtt ctgtcgggag gaccgggcgc
     1081 gcaggtggtg tcggcttttg acacggggcc cggaaacatg ctgatggatg cggccatggt
     1141 ctgggccacg aacggagagc agtgctacga cagggatgga gcgttcgcgg cgtcggcgcc
     1201 gtattccgag gctctgctgc aggaactgct gcagaccccc tatctgcgaa cggccccacc
     1261 gaaaacgacg ggccgcgaag actttggatg tacaagatgg gaagagataa aaaaagcctg
     1321 ggaaaagcaa atgagcgccg ctgaactggt cagcacgttc tgcgagttta cggctcgatc
     1381 gattgcggaa gccatcgtga ccttcgtggc gccgacctgg ccgccggatc gagtggtcgt
     1441 cgccggcggt ggagcttaca acccggagct ctgccgtcgc attcgtgagc gattgccgcc
     1501 gtgctgcgct tttgagcgat ccgatgtcta tggggttccg gtgatggccc gcgaggcgat
     1561 ggcctttgcc gcgctcggtg aggcttttct ccgcggccgg cccgccaacg ttccggctgc
     1621 taccggggcg cggcagccgg tcgttctcgg cagttacact ccggcttgac cgtcgggaga
     1681 ctactcgacg act
//
LOCUS       DAORHD010001201         1693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_94594_length_1693_cov_2.671551, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001201 DAORHD010000000
VERSION     DAORHD010001201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94594_length_1693_cov_2.671551"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="PKO06_20095"
     CDS             complement(<1..1643)
                     /locus_tag="PKO06_20095"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72018.1"
                     /translation="MIQTAEGALGESHSILQRMRELAIQASNDTLTSNDRLEIQKEVN
                     QLRDDLNRISRNTEFNTKKLLDGSQTALVSASTNSARGLVAGGGIDIGDFEVSIALVK
                     GGVSQMQRSQTFLVQGNGTLAKGNTELQSVAQMYDANGVFILQSPQTLTINGNGKSST
                     VTVDGQMTLDKLAAAIQNAIVGSSGLDIANTQVGMVNTAQSGVAGVGGYIELTSGIIG
                     EDGKIAFAADQRLIDAIGISQTRTAVNNQVEITARDANGNLRSTLTESNRAAGLLNGV
                     DLAFDSQAAQRAGTRGLEEGLTLDNDEFFTISVGNTSVTVTVVAGDWSLEGIARTINQ
                     QISDQGGADGFKAQVVDGQIRLSFEPMFASIASTIRITNAPTNSVLGVVEGTYSGFVQ
                     GQKDEGKKVWGFSQYISTVTYGVVTGSSLAYSVSDGISATTIVIISASLGSSSLTSDL
                     KRFAIFQASVNQTLAANGVDVRVDQVGAALAFTALRVGDDNTNAGVIQSLVSVKSSAG
                     STFLSQKFGMADGTSRGSGDTNFRVHVVDNTPQFQIGGDP"
BASE COUNT          364 a          503 c          437 g          389 t
ORIGIN      
        1 gggtctccgc cgatctgaaa ctgcggtgtg ttgtcaacga cgtggacgcg aaagttcgta
       61 tcgccactgc cgcgggaagt accatctgcc atgccgaact tctggctcag gaacgtgctt
      121 ccagcacttg atttgacgct cacgagactt tgaatgacac cggcgttcgt attgtcgtcg
      181 ccgacccgca gagccgtgaa agcgagagcc gctcccacct gatcgacgcg cacgtcaact
      241 ccattcgccg cgagcgtttg attgacggat gcctggaaga tcgcgaaacg tttgaggtca
      301 gaggtcagag aggaagaacc aagtgatgca ctgatgatga caatcgtcgt cgccgaaatg
      361 ccgtcactga cggaatacgc cagactggag ccggtcacca ccccataagt caccgtactg
      421 atgtactgcg aaaatcccca cacctttttg ccctcgtcct tctgaccctg aacaaagccg
      481 ctatatgtcc cttcaacgac gcccaggaca ctgttcgtgg gagcgttggt aatacgaatg
      541 gtcgaagcga tactggcaaa catcggttcg aaactcagac gaatctggcc atcgaccacc
      601 tgagccttga agccatcagc tcctccctga tctgatatct gctgattgat ggtgcgggca
      661 atgccttcga gagaccaatc ccctgccaca acggtcacgg taaccgatgt attccccacg
      721 gaaatggtga agaactcgtc gttgtccagc gtcagtccct cttcgagccc tctggttccg
      781 gctctctgcg ctgcctgcga gtcaaaggcc aaatcgacac cgttcagcaa acccgcggca
      841 cgattgcttt ccgtcaaggt actgcgcaga ttcccgttgg cgtcccgagc tgtgatttcg
      901 acctgattgt tcacggcagt cctggtctgc gagatgccga tcgcatcgat caggcgctga
      961 tcggcagcga aagcgatttt gccgtcttcg ccgataatcc cactggtcag ttcaatgtat
     1021 ccccccaccc cggccacacc actttgagcg gtattcacca ttcccacctg agtgttcgcg
     1081 atatccaagc cgctggaacc aacaatggcg ttctggatag ccgcagccag cttgtcgagg
     1141 gtcatctgac catcaaccgt cacggtgctc gatttcccgt ttccgttgat ggtgagtgtc
     1201 tgggggctct gaagaatgaa gacgccgttc gcatcataca tctgagccac actctgcagt
     1261 tccgtgttgc ccttcgcgag agttccgtta ccctgtacaa gaaaggtctg ggaacgctgc
     1321 atctgggaaa caccgccctt cacgagagcg atgctcactt cgaagtctcc aatgtcgatt
     1381 ccaccgccgg ccaccaggcc acgtgccgag tttgtactgg cactgaccag agcagtctgc
     1441 gatccatcaa gcagtttctt ggtattgaat tccgtattgc gggaaatgcg gttcagatcg
     1501 tcccgcagct gattcacttc tttctggatc tcgagacgat cgttactggt cagcgtatca
     1561 ttactggctt gaatggccag ttcacgcatg cgctgcagaa tactgtgaga ttcgccgaga
     1621 gctccctcgg cagtctggat catggagatg ccgtcctggg cgtttaaaat ggcccgactc
     1681 aatcctcgaa tct
//
LOCUS       DAORHD010001202         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95044_length_1688_cov_3.129822, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001202 DAORHD010000000
VERSION     DAORHD010001202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95044_length_1688_cov_3.129822"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="PKO06_20100"
     CDS             <1..1359
                     /locus_tag="PKO06_20100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72019.1"
                     /translation="YYLGMILAREGSISKAEGHLRQAAMLIPDSPDIMYQLSDVLSQM
                     NSEEAAQESEELLKKVLELNPAHAGALARKGLQAAQTGDHDQAIEALIKARDADPRNR
                     ELQLELGRALLAKGRAPEALEPLKIATQLNPADIEGQQLLGKCYEALNRPNEAAAAFA
                     LAKGKGKTAAAGKELVGYDLARNLAITGRAQEAIAEYQKMIKNASDPATGWAELGQLY
                     QDLKERDKAISAYKKWFEIGGASEAAYRVAQIERESGNLEKAHEALSLITRQKDEWGE
                     RAKEELADIERELQEKERETLLEQADVGDEKNREKAFLKLLEMDKKDRDALEGLLELS
                     KVRGDLEQAKYYTNELKKAGYLSKSEAKQAAEGLEAKQDAGDDIAAWEARLEDCKRDD
                     DYDGAIEELLKLKAYSKSQLDAMKGSGDPSVKTTLRSRIQDYNRELKELRAKKRANRK
                     KK"
     gene            1388..>1688
                     /locus_tag="PKO06_20105"
     CDS             1388..>1688
                     /locus_tag="PKO06_20105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72020.1"
                     /translation="MMRKGFSLIELLVVVVIIALLIGVAAPYYADYVKESKLSKAKAD
                     LDVFKQAVTLYNSREDLPYMGVIATQSPYLPILGENDFVGLQGQYLTNIPLDPWSK"
BASE COUNT          469 a          387 c          515 g          317 t
ORIGIN      
        1 tattacctgg gcatgattct ggcgcgtgag ggatcgatca gcaaggctga gggacattta
       61 cgtcaggctg caatgctgat tccggattcc cctgatatca tgtaccagtt gtcggatgtg
      121 ttgagccaga tgaacagcga agaggcagct caggaatcgg aagagttgct gaaaaaggtt
      181 ttagagctga acccggcgca tgccggagca ttagcgagaa agggactgca ggcggcgcag
      241 acgggtgatc acgatcaggc cattgaagca ctgatcaaag ctcgtgacgc ggacccgcga
      301 aatcgagaac tgcaactgga attgggccgc gccctgttgg ccaaggggcg ggctcccgaa
      361 gcactcgaac cgttgaaaat cgccacacag ctgaatcctg cagatatcga aggtcagcag
      421 ttgctgggga agtgttatga agcgttgaat cgccccaatg aagcagcggc cgcttttgct
      481 ctcgcaaagg gcaaagggaa aaccgcggcg gcgggaaaag aactggtcgg gtatgatctg
      541 gcacgcaatc tggcgatcac cggtcgggcg caggaggcga tcgccgaata tcagaagatg
      601 ataaaaaatg cttctgatcc ggctacgggc tgggctgaac tcggacagtt gtatcaggac
      661 ctcaaagaac gcgacaaggc aatcagtgcc tacaaaaaat ggtttgaaat cggtggcgct
      721 tcagaagcgg cctatcgtgt ggcgcagatc gagcgcgagt cgggaaatct ggaaaaagct
      781 catgaagctc tgtctctcat cacgcgccag aaagatgaat ggggcgagcg ggccaaggaa
      841 gaacttgccg atatcgaacg agagctacag gaaaaagaac gcgagacgct tctggagcag
      901 gcggacgtcg gggacgaaaa aaaccgtgag aaagcttttc tcaagctgct ggaaatggac
      961 aagaaagacc gtgatgctct cgaagggctc ctcgaactgt cgaaggttcg cggggatctc
     1021 gaacaggcca aatactatac gaacgaattg aagaaagccg ggtatctgag taaatctgaa
     1081 gccaaacagg cggccgaagg tctcgaggca aaacaggatg ccggggatga tatagccgcc
     1141 tgggaagccc gtctggaaga ctgtaaacgc gatgatgatt atgatggagc gatcgaagag
     1201 ttgttgaaac tcaaggccta ttccaagtcg cagctggatg ccatgaaggg ttcgggtgac
     1261 ccgagcgtga aaacgacact ccgatcacgc attcaggatt acaaccggga actgaaggaa
     1321 ctgcgagcga agaagcgtgc gaatcgcaag aagaagtaag ctcaaaggct gagcaaggag
     1381 tctcgatatg atgagaaaag gtttctcgct gattgaactg ctcgtcgtgg tggtcatcat
     1441 tgccctgttg atcggggtcg cggcacccta ctatgccgac tacgtgaaag agtcgaagct
     1501 gagcaaagca aaagccgacc tggacgtatt caagcaggcc gtgactttgt acaattctcg
     1561 cgaagatctg ccttatatgg gagtgatcgc gacacagtcg ccttatctgc ctattctggg
     1621 cgaaaatgac ttcgtcggct tgcaggggca gtatctgacc aacatacctc tcgatccgtg
     1681 gagcaaaa
//
LOCUS       DAORHD010001203         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95065_length_1688_cov_2.705450, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001203 DAORHD010000000
VERSION     DAORHD010001203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95065_length_1688_cov_2.705450"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="PKO06_20110"
     CDS             complement(<1..1387)
                     /locus_tag="PKO06_20110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72021.1"
                     /translation="MKTILINPPFVKDFCRTQRWAARTRGRVLRAPDWLANATAVLEQ
                     AQIPARLYDFPALSWDKQQLSQLVAREQPDVVVLDTTTPSIYSDLECARLIKSQNNAK
                     VILVGTHVSALPDETLRLANGAVDVACIGEYDETVRDLILADFAPTVPGTVSWSEGKA
                     VHGPARCLIDDLDRLPFPAWHHLDLFTYFDGGKLYPYLDIFSGRGCPNACSFCLWPHV
                     MHGTRYRFRAPVRVVDEIEHDLRLCPEVQRTGEFFFEDDAFLVRRDRAAEICEEILRR
                     NLRVTFSVNVRVDQADAEVFRLMKRAGCRELLVGFESGHQPLLNSMNKRTNLDQAFSF
                     MSLAHHAGLEVHGCFVIGLPGETLESGEKTIDFALQLGLDTVQFSGAAPFPGTPFYDE
                     VKKNGWLTAHDWHEWLRDGEQTALVSYPQLSASDIDTLVNRGLKRFYFRPSFMLRFLW
                     NTRNFKDLKFLV"
     gene            complement(1398..>1688)
                     /locus_tag="PKO06_20115"
     CDS             complement(1398..>1688)
                     /locus_tag="PKO06_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72022.1"
                     /translation="LDPRPQPNILWLERTLDVNSGLYIGVGFLATGGLIFLTILLTWI
                     SRSFGDLQAIRPAILGLTFVFLGLQIGFSSFLWGLFQIQRLPPSGDPPSHSS"
BASE COUNT          442 a          437 c          480 g          329 t
ORIGIN      
        1 acaccagaaa cttcaagtcc ttgaagtttc tggtgttcca gagaaagcgc agcatgaagg
       61 acggacgaaa gtagaaccgt ttcaaaccgc gattcaccag agtatcaata tctgaggctg
      121 aaagctgagg atacgatacc agggctgtct gttcaccgtc acgcaaccat tcatgccaat
      181 cgtgcgccgt gagccacccg ttcttcttta cctcatcgta aaaaggtgtt cccgggaaag
      241 gggcggctcc ggaaaactgt acggtatcga gcccgagttg cagagcgaag tcgatcgtct
      301 tctcgccaga ctcgagagtt tcaccgggga gaccgatgac aaaacagcca tgaacctcca
      361 ggccagcatg atgggcaaga ctcatgaagg aaaatgcctg gtccaggttc gtgcgtttgt
      421 tcatgctgtt gagaagcggc tgatgacccg attcaaaccc gacaagaagc tcacggcagc
      481 cggcgcgttt catcagacga aagacttcgg cgtctgcctg atcgacgcga acattcacgg
      541 aaaacgtgac ccgcagattt cttctgagga tttcctcgca gatctctgcc gcacgatcac
      601 ggcgcacgag aaaagcatca tcttcaaaga aaaactcgcc ggttctctgc acctctgggc
      661 agagcctgag atcatgctct atctcgtcga caactcgtac tggagcgcga aaccgataac
      721 gtgttccatg catcacatgc ggccagagac agaaactgca tgcattcgga caaccacgtc
      781 ccgaaaagat gtcgagatac gggtacaact tgccgccgtc aaaatacgtg aaaagatcca
      841 gatgatgcca ggcgggaaat ggcaatcggt ctaggtcgtc gatgagacag cgggcgggtc
      901 cgtgaacggc ttttccttcg ctccacgaca ccgtgcctgg aacggttggg gcaaagtcgg
      961 caaggatcag gtcgcgtacg gtctcgtcat attctccgat acaagccaca tcgacagcac
     1021 cgttggccag acgtaacgtt tcatccggta aagccgaaac atgtgttccg accagaataa
     1081 ccttggcgtt gttctgtgac ttgatgaggc gggcgcattc gagatctgag tatatcgagg
     1141 gggtggtcgt atcgaggaca acgacgtcgg gctgctcacg agcgacgagt tgagagagtt
     1201 gctgtttgtc ccaactcagg gcaggaaaat catacaacct ggccggaatc tgggcttgct
     1261 caagcaccgc tgtggcattg gcaagccaat caggagctcg aagaactcga ccgcgcgtgc
     1321 gtgccgccca ccgttgagtt cggcagaagt ctttgacgaa cggtgggttg atgagaatgg
     1381 tcttcatgca cagcgattta cgaagaatgt gacgggggat cgccgcttgg cggcaaccgt
     1441 tgaatctgga acagacccca cagaaaggaa gagaaaccga tctgcagacc aaggaagacg
     1501 aacgtgaggc ccagaatcgc cgggcgaatg gcctgaagat cgccaaatga gcgagagatc
     1561 cacgtcagaa gtattgtcag gaagatcaaa ccacctgtgg ccaggaatcc gactccaatg
     1621 tagaggccgg agttcacgtc gagggtacgt tccaaccaga gaatattcgg ctgggggcgg
     1681 ggatcaag
//
LOCUS       DAORHD010001204         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95333_length_1685_cov_2.968712, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001204 DAORHD010000000
VERSION     DAORHD010001204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95333_length_1685_cov_2.968712"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1475
                     /locus_tag="PKO06_20120"
     CDS             306..1475
                     /locus_tag="PKO06_20120"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72023.1"
                     /translation="MGAARRRSLESSIREDLTRKFVFLSGPRQVGKTTLARTIIESER
                     GKYLNFDDPDDREDILLRRYTGYSLVCLDEFHKYPRWKAHIKGVYDKYSQTLRLILTG
                     SARLDVYQKSGDSLLGRYYLHHLHPLTLGELHRAVMPQLPATEALRQPCSAGQPGFDE
                     LMRFGGFPEPFQRQSDTEHRRWSNMRTSLLVQQDLRDLSEVKLLSLAEQLIALLPERV
                     GSLFSARSLSENVQVSTPTITHWMDIFSRLFIVYKLMPYSQGLGRALHRQPKYYFWDW
                     SQVKDEGARFENAVAGHLFKAAQYWTDHGVANVEVQFIRDREGREVDFLMVQNGRPWF
                     LVECKIAETSFTHALAFYSGKLGIPGIQLLQKSGVARNQGNLLLISADRWLGHFP"
BASE COUNT          385 a          432 c          454 g          414 t
ORIGIN      
        1 cacccgcgag tatgtgcccc tggtcatgta tcaaaaaggg aaaaccggtc gtgatctcgg
       61 tacccgcaac ggcttctggg acatcgccaa aaccgtcgcg gttcatctcg gtatcgacta
      121 tccgcatgga aggtcctgcc tgggctgaca gaccggaact cctgagagct gttcggcgac
      181 aggcgcggat tcccgcttcc gacgaagatt cgccggccag gttcggcatt tgtaaagcct
      241 attttacaaa tggcatgcca aatgataagt tgtagtgaca tttgccgctg gaggattttt
      301 gttacatggg tgccgctcgt cgtcgttccc tggaatcatc gatccgggaa gatctgacaa
      361 gaaagttcgt ctttctttca gggccgcggc aagtcggcaa aacgacactt gcccgcacca
      421 tcattgaatc agagcgagga aagtatctta attttgacga tccggacgat cgtgaagaca
      481 tcttactgag aagatacacg ggatactccc tggtttgtct cgacgagttt cacaagtatc
      541 ctcggtggaa agcacatatc aagggcgttt acgacaagta ttctcaaacg ctgcgcttga
      601 ttctgaccgg tagcgcacgc ctggatgtct accaaaagtc cggcgacagc cttctagggc
      661 ggtattatct tcatcactta caccctctga cactcggcga attgcatcgc gcggtcatgc
      721 ctcaactccc ggcgactgaa gctcttcgac agccctgctc ggccggacag ccaggctttg
      781 atgagctcat gcgcttcggc ggttttcctg aaccatttca gcgacagtct gacactgagc
      841 atcgacggtg gtccaacatg agaacttcct tgcttgtaca acaggacctt cgtgatctat
      901 cggaagtgaa gctgctgtcg ctggctgagc agttgattgc acttctgccg gaaagggtcg
      961 gctcactgtt cagtgcgaga tcgttatctg agaatgttca agtttcgacc ccgacgatca
     1021 cgcactggat ggatatcttc tcgcgcctgt ttattgtgta caagctcatg ccgtattcgc
     1081 aagggttagg aagagcacta catcggcaac caaagtacta tttctgggat tggtcacaag
     1141 tgaaggatga gggagcccgc tttgaaaacg ccgtagcagg acatttgttc aaggctgccc
     1201 aatattggac cgatcacggt gttgccaatg tcgaggttca gttcattcga gaccgggaag
     1261 gtcgcgaagt tgattttctg atggtgcaga atggacgacc ctggttcctg gtcgagtgca
     1321 agattgctga aacatctttc actcacgcac ttgcgtttta ctcgggcaaa ctcgggattc
     1381 ccgggattca actgcttcaa aagtcgggcg ttgcgcgaaa ccagggaaat ctattgttga
     1441 tcagtgctga tcgatggctg ggacactttc cgtaagatct tctctcttca tcacgctaaa
     1501 atgaaaccgc ccccccgaag cctgggcttc aggggggcgg ttttgtccga gttgatttcg
     1561 gcttagggcc gcacagcatc aacagagatg aactgaccaa ctccagggct cggttcgggg
     1621 ttgatcatgg tcgtgcccga gatgttgaac gaacccgacg cttctgaagg tgaaccagga
     1681 attgg
//
LOCUS       DAORHD010001205         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95395_length_1684_cov_3.799263, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001205 DAORHD010000000
VERSION     DAORHD010001205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95395_length_1684_cov_3.799263"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKO06_20125"
     CDS             complement(<1..919)
                     /locus_tag="PKO06_20125"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV72024.1"
                     /translation="MKRTILLLKLLIVLLISVAMSAAHRSGKLNVFEGYWSDIQRSIT
                     WTIESRVLDVASAPLRVFAFPIDGASIRDFAKEGVSWPFPRSLYAQAVKRLESLGVRA
                     VAFDVFFSQHDTAPGLDEFVDTLASSSVKVVLAATLEDAGASLQSVKTVEDDDDDEEY
                     GFQKTRRIITLPHSRLREKAWGIGLINSELNINYDRIFRRQPPAYFHEGRWFPNLGVA
                     LVQATRDKTGAIELATGQMRLLDGKTVPLEPVVASQSYIGAEWAAEPRWRNHGGSIDQ
                     GCGRYFFSNFYNLVKGVNLWSTEKLKDAVV"
     gene            complement(929..1624)
                     /locus_tag="PKO06_20130"
     CDS             complement(929..1624)
                     /locus_tag="PKO06_20130"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNV72025.1"
                     /translation="MAVEKPDLLLAGGDLLLGHAGSLRRLAEEWPDSGLQYLILSDSP
                     LARPTIRDLLPSQPAWVRPANLPPGAMGPRFCLWHAGNERLGIVSLVTDSDRSVDDVF
                     STLANLLQVDLRGVPTLVFAHGADLNLKQALSWRFSSHETPVHVLGMGLGVATADEQI
                     VSGRAWISDIGGVGRAGGIGGSDPDVWWKRHREKIPLEAAPPVGRLMADAVCLELGLS
                     GKCSAIRRQRIGG"
BASE COUNT          383 a          539 c          443 g          319 t
ORIGIN      
        1 taaccacggc atccttcagc ttctctgtcg accagaggtt gacacccttc accagattgt
       61 aaaaattgct gaagaagtaa cggccacaac cttgatcaat actgccgcca tggttccgcc
      121 accgcggttc agccgcccac tcagcgccta tgtagctctg cgacgcgacc accggttcaa
      181 gcggaaccgt ctttccatca agaagtcgca tctgccccgt tgccagctct atggctcccg
      241 tcttgtcgcg agtggcctgg acgagtgcca ccccaagatt cggaaaccat cgcccctcat
      301 gaaagtaagc cggcggttgg cggcgaaaga tccgatcgta gttgatgttc aattcagagt
      361 tgatcaaccc gattccccaa gctttttcgc gcaaccgaga gtggggcaga gtgattattc
      421 gacgagtttt ctgaaatccg tattcctcgt cgtcgtcatc gtcttcgacg gttttcaccg
      481 actgcagcga tgctcctgca tcctcgagag tggctgccag caccaccttc acggaactgg
      541 aagccagagt atcgacaaac tcatccagac ccggcgccgt atcatgctgg gagaagaaga
      601 catcaaaggc caccgctctg acacccagtg attccaggcg tttcaccgcc tgggcataca
      661 gactgcgtgg aaacggccac gagacccctt ctttggcaaa atcccgaatc gaagctccgt
      721 cgatcggaaa cgcgaacact ctcagcggtg ccgacgcaac atcaagaact cgagattcaa
      781 ttgtccaggt gatcgaacgc tgtatatcag accagtagcc ttcgaagaca ttcagttttc
      841 cggaccggtg ggcggcgctc atggcaacac tgatcagcag gacgatcagc agtttcagca
      901 gcagaatcgt tcgtttcatc gaccagactc acccaccgat ccgctgtcgt ctgatcgccg
      961 aacactttcc cgaaagtccg agttcgagac acactgcatc ggccatgaga cggccaacag
     1021 gaggtgcagc ttcaagagga attttctcac gatgccgttt ccaccagaca tccggatcac
     1081 tcccgccgat tcccccggcc cgcccgacgc cgccaatgtc gctgatccag gcacgcccag
     1141 acacgatctg ttcatcggcg gtcgcgaccc ccagccccat gcccagcaca tgcaccggtg
     1201 tctcgtgtga agagaatctc caggacagcg cctgcttgag gttgagatcc gctccatgcg
     1261 cgaagaccag cgtgggaaca ccgcgcagat cgacctgcag caggttcgca agcgttgaaa
     1321 acacgtcatc gacgctgcgg tccgagtcgg tcaccagtga aacgatgccg agacgctcat
     1381 tcccggcgtg ccacaaacaa aaccgcggcc ccatcgcccc gggcggcaga ttggcgggtc
     1441 tcacccacgc aggttgagaa ggcagcagat cgcgaatggt tgggcgggcg agcggactgt
     1501 cgctcagaat cagatactgc agtcccgagt ccggccactc ctctgccaga cgccgaagag
     1561 agccggcatg ccccaacaac agatcgccgc cggcgagcaa cagatcgggt ttttcgactg
     1621 ccacaccggc gtgggcagct gagtccgttc gcgcagactc agttcatgca ggattctgcc
     1681 gagg
//
LOCUS       DAORHD010001206         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95524_length_1683_cov_3.264742, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001206 DAORHD010000000
VERSION     DAORHD010001206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95524_length_1683_cov_3.264742"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="PKO06_20135"
     CDS             complement(<1..573)
                     /locus_tag="PKO06_20135"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNV72026.1"
                     /translation="MSHLSHLEGYRISSGRLHFSGCDTVALVEQFGTPLYVISEDIAR
                     EKCRRYVQAFKSQQVQATVAYAGKAFLTTGFCGLLKNEAMGLDVCSGGELYTARAAGF
                     PMDRVLFHGNNKTIEEIRLALEFGVARFVVDNLHELHLLNDLAAQRGIVQSIFFRATP
                     GINTHTHSHIQTGHLDTKFGFGIENGQAEEA"
     gene            complement(709..>1683)
                     /locus_tag="PKO06_20140"
     CDS             complement(709..>1683)
                     /locus_tag="PKO06_20140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV72027.1"
                     /translation="YRDRTDTYVTQVTDLTADLDKIHQKLFELSADGGGDGPESVNAA
                     LHDAVNKISWSPKGEKVYRVVFLVGDAPPHMDYQDDVKYLDTCKAAVLKDIVINTIRC
                     GSDSQTETTWKEIASRAEGRYTSIAQSGGVQVVSTPEDARLAELSGKLMTSGVYYGSK
                     EEREMVQASLNRAESESKKAKESMDSSALAMEADKAEFRGKMAGAAAAPSASAEMSGM
                     GMGSHVGRADLLALYQKEGDAGLAKIKDTDWPDDMKKLSATERVDFIKRKIAERQELE
                     KQIEDASKKRSEFLREQQKKSGSGDAGFDAVVLDMLREQGAKRGIEYK"
BASE COUNT          310 a          516 c          457 g          400 t
ORIGIN      
        1 cgcttcttcc gcctgtccgt tctcgatgcc gaacccgaac ttggtgtcga ggtggcccgt
       61 ttgaatatgt gagtgggtgt gcgtattgat gccaggcgtc gcacggaaaa agatagactg
      121 gacgattccc cgctgtgccg ccaggtcatt cagcagatgc agttcgtgca ggttgtcgac
      181 cacgaagcgt gcgacgccga actcgagggc cagacgtatc tcttcgatgg tcttgttgtt
      241 gccatgaaac agcacgcgat ccatggggaa gccggccgcg cgcgccgtgt acagttcgcc
      301 ccccgagcag acatcgagcc ccatcgcttc gttcttgagc aggccgcaga acccggtggt
      361 cagaaaggct ttcccggcgt atgccaccgt ggcttgtacc tgctggcttt tgaacgcctg
      421 cacataccgt cggcatttct ctcgggcaat atcttcgctg atcacataca gaggcgtacc
      481 gaactgctcg acgagagcga cggtatcaca gccggaaaaa tgcagccgac cactactgat
      541 ccggtatcct tcaagatgcg agagatgtga cataagactg ctccgatatg ttggtcattc
      601 ctgcttcggt cgttcgtatc aggggcgcgc atctcatacc atcatctgcc ccggaaacac
      661 aagaggaccg aaagtctaca ctttcggtcc cccgtgtaaa ctggcgaatt acttgtattc
      721 gattccccgt ttggcaccct gctcacgcag catgtccaag acgaccgcat cgaacccggc
      781 atctccgctg cccgacttct tctgctgctc gcgcaggaat tcagagcgct tcttgctcgc
      841 gtcttcgatc tgcttctcga gttcctgtcg ttcggcgatc ttgcgtttga tgaagtcgac
      901 gcgttccgtc gcgctcagct tcttcatatc gtccggccaa tcagtatcct tgatcttggc
      961 caggccggca tcgccctctt tctgatacag ggccagcagg tcggcacgtc cgacgtggct
     1021 gcccatgccc ataccggaca tctcagccga ggctgacgga gccgcggctg ctcctgccat
     1081 tttgccccgg aactccgctt tgtcagcttc catcgccaga gctgaagaat ccatcgactc
     1141 tttggctttc ttcgactcgg actctgcccg attgagcgaa gcctgcacca tttcacgctc
     1201 ttctttcgag ccgtagtaca cgccgctggt catcagtttc cctgagagtt cggcgaggcg
     1261 cgcgtcttcc ggagtcgaca caacctgtac gccaccgctc tgcgcgatcg acgtatagcg
     1321 accttcggct cggctggcga tctctttcca ggtggtttct gtttgagaat cgctgccaca
     1381 gcgaatcgtg ttgatgacga tgtctttcaa caccgcggct ttgcaggtgt cgagatactt
     1441 tacgtcgtcc tggtaatcca tgtgcggggg ggcgtcgccg acgagaaaca cgacgcggta
     1501 gaccttctca cccttgggtg accacgagat cttgttcacc gcatcatgca gagcggcatt
     1561 cacgctctca gggccgtcac caccgccatc cgcgctgagt tcgaacagct tctgatgaat
     1621 cttgtcgaga tcggccgtca gatcggtgac ctgggtgaca taggtgtcgg tgcggtcgcg
     1681 ata
//
LOCUS       DAORHD010001207         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95634_length_1682_cov_3.167179, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001207 DAORHD010000000
VERSION     DAORHD010001207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95634_length_1682_cov_3.167179"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKO06_20145"
     CDS             complement(<1..248)
                     /locus_tag="PKO06_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72028.1"
                     /translation="MNRISVSTSSLLLVMLFAFAGLDTCWAAIGCTLNNPAQDLKFLF
                     PEVTSFREEVRDFPRLAEGKKLYAALQERLGSDLDPVY"
     gene            complement(259..1503)
                     /locus_tag="PKO06_20150"
     CDS             complement(259..1503)
                     /locus_tag="PKO06_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72029.1"
                     /translation="MTRSSLVTGLVFCAFWAFQMGVPGNSAFGQAADPFADLTLTIAS
                     SPSFTASRPAASRTRTFLKKELISQFTWEDGSRTPYSRQSIGGELLQRFSDDRRTWGS
                     FNLQGRLVHRNAFRPVLNDMEGESRSNWFAEYHNVYFDLFNALDPFLSPEARRDNLGR
                     YHFRIGRFYLPSGINLQTDTHGTVLQLSNDRNFGFERDWYAGFWGGLSPHVSYDLYAM
                     AGSGYDLRLEGQKGLLGLRLSLSSRSLFERGLEGGLSLLTGERLSSHALMRSPSVAAQ
                     ADRGRFIETRRYGADIRQTRPVSGGTLISTGEWSTGRDESDNVFTQLYQFEFLRRDRK
                     LGWALQYRRFFQDIGSDPMMPEMTVPGKTDASLAGEVTWYFRNDVGNSNLHWIKLNIE
                     RQLERQSGRAAVLTTLNYYRYW"
BASE COUNT          373 a          458 c          515 g          336 t
ORIGIN      
        1 tcataaaccg ggtccaggtc gcttcccagc cgctcctgga gagccgcata caactttttc
       61 ccttcggcga gccgggggaa gtcccggacc tcttcgcgaa aactcgtcac ttccgggaag
      121 agaaatttga gatcctgggc cggattgttc aacgtacagc cgattgctgc ccagcaggtg
      181 tccaggccgg caaaagcgaa taacatcacc agaagcaggg aagaagttga gactgatatg
      241 cgattcatgc gaaactcctt accagtagcg gtagtagttg agagtggtca ggaccgccgc
      301 ccgtccgctt tgccgctcga gttgtctctc gatgttgagc ttgatccaat gcaggttgga
      361 gttgcccaca tcgttgcgga agtaccaggt gacttcaccg gcgagcgacg cgtcggtctt
      421 tccgggaacg gtcatctccg gcatcatcgg gtccgaaccg atgtcctgga aaaaacgtcg
      481 atactgaagc gcccacccga gtttccgatc gcgtcgcaga aattcgaact ggtacaattg
      541 cgtgaacacg ttgtccgatt cgtcacggcc ggtcgaccat tcgccggttg agatcagggt
      601 tccaccggat accgggcggg tttgccggat gtcggcgccg tatcttcggg tttcgatgaa
      661 ccgcccgcga tcggcctgcg cggctacgga cgggctgcgc atcagagcgt gcgatgacaa
      721 ccgttcaccg gtcagcagag aaagccctcc ttcaagtccg cgctcgaaca gagaacggct
      781 ggaaaggctg agccgtagac ccagcaagcc tttctgccct tccagtcgca ggtcgtagcc
      841 tgatccggcc atagcgtaca gatcgtagga gacgtgtgga ctcagcccgc cccagaaacc
      901 ggcataccag tctcgttcga atccgaagtt ccggtcgttg gaaagctgaa gcacggtgcc
      961 gtgagtgtcg gtctgcaggt tgattcccga cgggagatag aatctgccga tgcggaaatg
     1021 gtaccggccc agattatcgc gacgcgcttc cggagagaga aaggggtcga gagcattgaa
     1081 caggtcgaag tagacattgt gatattcggc gaaccagttg ctgcgcgact ctccctccat
     1141 gtcgttcaga acagggcgaa aagcgttgcg atgcaccagt cgtccctgca gattgaaact
     1201 gccccaggtt cgtcgatcgt cggaaaaccg ttggagaagc tcgccgccta tggattggcg
     1261 agagtaggga gtccgtgatc cgtcttccca ggtaaactgc gaaatcagtt cctttttgag
     1321 aaaggtgcgg gtacgagaag cggccggtcg agatgccgta aaggaggggg aagaggcgat
     1381 cgtgagagtc aggtcggcga acggatcggc agcctgcccg aaggcggaat tgcctgggac
     1441 tcccatctgg aaagcccaga acgcgcagaa taccagaccg gtgacgagcg aggaacgtgt
     1501 catgttgttc tcctgggcag agtcgctggc aggttctgcc tttgattact gagttgcaat
     1561 aacgatgcca agaaagccgg tgacagctcc atcgcgacga gaatgcgagg caacccggct
     1621 ttcacaggga tttagttcga gcgaatcacc ctcgcgcggg tgtcgggatg ccaagctttc
     1681 tc
//
LOCUS       DAORHD010001208         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95691_length_1681_cov_3.788438, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001208 DAORHD010000000
VERSION     DAORHD010001208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95691_length_1681_cov_3.788438"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..742
                     /locus_tag="PKO06_20155"
     CDS             41..742
                     /locus_tag="PKO06_20155"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HNV72030.1"
                     /translation="MGSAQSGWQASRQWSVLHSDSPDRGRQNREENPEAGGSAVTVQN
                     PEHAKARLFAQAMSRSEPGATRTFRARGSSMYPLIRDGDTIELSKSDPARARLGDILV
                     TDHETQVLCHRLIRKQRRGNTWWYITQGDTLLFEDAPIPADRVLGLVRSLRTPSSTFH
                     DLDSPSGRAAKRLFLMFPLLKRCVTNVCAAVGVQLDAPDTSRSFQRFLQSVYLLCCLP
                     VYAFHQLSRVTHPLR"
     gene            complement(768..>1681)
                     /locus_tag="PKO06_20160"
     CDS             complement(768..>1681)
                     /locus_tag="PKO06_20160"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HNV72031.1"
                     /translation="VAVRVISEPIEPVNPNRPTYSRYMIFSILIGLLAGGLLAYLQEN
                     FDNSLKSTDDAQQCLGLVNLGSLPDLKHLLNVPTDPKVEIAPALVTFHHRSSPEAEAF
                     RTLRTTLLYKAQQHPFKTLILTSWGRNMGKSTVTANIAMTYAQSGMKVILVDCDMHKP
                     TLHHLFFLQNHRGLTDLIMGDHLEKVLKPTAQPNLFVIPTGPLPPNPSELLGSQSMDV
                     AIRLLSASADLVIFDMPPLMAVADSAVLAGKVEAVLFLVPIGKESRRYALKGLDLLRL
                     VKAPIVGMVCNFLKRHEFEGYRYHYHV"
BASE COUNT          362 a          466 c          491 g          362 t
ORIGIN      
        1 tcgcctcgct ctccggaggc acctgggaaa gtcgtgtcac gtgggatctg cgcaatcagg
       61 atggcaagcg agtcgccaat ggagtgtact tcattcagat tcacctgacc gcgggcggca
      121 aaaccgagaa gaaaaccctg aagctggcgg ttctgcagtg acggtgcaaa accccgaaca
      181 cgccaaggca cgtctgttcg cgcaggccat gtcacgaagt gagccgggag caacccgtac
      241 gtttcgcgcg cgtggcagca gcatgtatcc gctgattcgc gatggcgata cgattgaatt
      301 gtccaagtca gatccggcgc gggcacgatt gggagatatt ctcgtgaccg atcatgaaac
      361 ccaggtgttg tgccatcggt tgattcgtaa acagcggcgc gggaacacct ggtggtacat
      421 cactcagggc gatacgctcc tcttcgaaga cgcgcccatt cccgccgatc gcgtcctggg
      481 cctggtccga tctctgcgca cgcccagctc gaccttccat gatcttgatt cgccgtctgg
      541 tcgtgcagcc aaacgcctgt tccttatgtt tcctcttctg aagagatgtg tgaccaatgt
      601 ctgcgcggca gtcggcgttc aactcgacgc gcccgataca tctcgctcgt ttcagcggtt
      661 cctgcagagc gtgtatcttc tctgttgcct gcccgtgtac gcctttcatc agctgagtcg
      721 tgtgacacac ccattgaggt gacgtcgaca acgacgccgg cggcgactca gacgtggtag
      781 tggtagcggt agccctcaaa ctcgtgacgt ttcaggaaat tacacaccat acccacgatc
      841 ggggccttga ccagccgtaa caggtcgagc cccttcaatg cataccgtcg gctttccttg
      901 ccgatcggca cgagaaagag cacggcttcg accttgcccg ccagaaccgc tgagtccgcg
      961 accgccatga ggggcggcat atcgaagatg acgaggtcgg ccgaagcaga gagcagtcga
     1021 atggcgacat ccatgctttg cgaacccagc aactcggacg gattcggcgg cagaggtccg
     1081 gtgggtatga cgaacagatt cggttgtgcc gtcggcttca gaaccttctc gagatggtcc
     1141 cccataatca gatcggtgag gccacggtga ttctgcagaa agaacagatg gtgcaaggtt
     1201 ggtttgtgca tgtcgcagtc aacgagaatg accttcattc ctgactgtgc gtaggtcatg
     1261 gcaatatttg ccgtgaccgt cgactttccc atattgcgac cccacgacgt cagaatcagc
     1321 gttttgaagg gatgttgctg cgctttgtac aagagagtgg tgcgcaaggt gcggaaggct
     1381 tcggcctctg gagatgatcg atgatgaaag gtcaccaggg ctggagcaat ctcaactttg
     1441 ggatctgtcg gtacgttcag caggtgcttg agatcgggaa gactgccgag attcaccagt
     1501 ccgagacact gctgcgcgtc gtcagtagat ttcagactgt tgtcgaagtt ttcctgcaaa
     1561 taggccagta acccacccgc cagcaagccg atgagaatgc tgaagatcat gtaccgcgaa
     1621 taggtgggac ggttcggatt caccggttca atcggttcac tgatcacgcg aacggcgacg
     1681 c
//
LOCUS       DAORHD010001209         1679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95866_length_1679_cov_4.035099, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001209 DAORHD010000000
VERSION     DAORHD010001209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95866_length_1679_cov_4.035099"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PKO06_20165"
     CDS             <1..191
                     /locus_tag="PKO06_20165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72032.1"
                     /translation="LVSFFGVGFFASLASQLWPWALSRATFDSVFGIAIGSVYFLLRW
                     FLIFRLGPPLRPARRRKD"
     gene            436..1017
                     /locus_tag="PKO06_20170"
     CDS             436..1017
                     /locus_tag="PKO06_20170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72033.1"
                     /translation="MFESVRMAVLAWARDRWWLPRAALMLYLAFISLELLSDSTTWTW
                     FTPVNLGIHETGHVVFRLTGWRFIEIAGGTIAQLLAPLFLIVSFFRQFDLFAPAFGAF
                     WLGTNLHNISVYMRDARARALPLVTVGGGNHIIHDWHYLLGQLGLLSWDTLLGSLVWL
                     LSFMIMGSACAYGLWVCRLIFEANQQTPLAENH"
BASE COUNT          317 a          499 c          451 g          412 t
ORIGIN      
        1 tgttggtcag tttttttggg gtcggcttct tcgcgtctct ggcctcacag ctctggccgt
       61 gggctctgtc acgcgccacc ttcgacagcg tgttcggcat cgccattggc tccgtgtatt
      121 tcctgttgcg ctggttcctg atcttccgtc tcggtccgcc gctccggccc gcacggcggc
      181 gaaaagactg agcgccactc cagaggagca ccttgcctcg aagcgattcg ccggcgcgtt
      241 gagcgcatga gacgatccgg cgaacccaag ccaccccagc gcgtaaccag gccgccaact
      301 gtcttgcaaa ggttccgctc attcatgcgc acccgatcat gctagaatgc gaaccgttgc
      361 cgttgcgccg atgaggcgcg gcatgagcct ctcattctgc gaggcgcacg aagagcggcg
      421 gtgatcagga acctcatgtt tgaatcagtg agaatggcgg tgctcgcctg ggcgcgggat
      481 cgatggtggc tgccacgcgc ggcgctgatg ctttatctcg ctttcatcag cctggaactt
      541 ctttcagata gcacgacctg gacgtggttc acccccgtga acctcggtat tcacgagacc
      601 ggtcacgtgg tgttccgcct caccggatgg cgtttcatcg agatcgccgg cggcacaatc
      661 gctcagctgt tggcgccact ctttctgatc gtttcgttct tccgccagtt cgatcttttc
      721 gctccggcct ttggcgcctt ctggctcgga accaacctgc acaacatctc ggtgtatatg
      781 cgcgatgccc gagccagagc cctgcctctg gtcaccgtcg gcggcggaaa ccacatcatt
      841 catgactggc actatctgct cggtcagctc ggcctgcttt catgggatac cctgcttggc
      901 agcctggtct ggctgctctc gttcatgatc atggggagcg cctgcgccta cggtctctgg
      961 gtctgccggc tgatcttcga agccaaccag caaacgcccc tggcagagaa ccactgacgg
     1021 gcacttccgc ggggattcgt gacctgccac cagtgaactt cccggacact tctgtgcgaa
     1081 tgtccgagtc gctaaagcac aaaacccgct ttatcagcgg gttttctcga gactacaata
     1141 gccccagcgg ctatcgaatt agatgcctgt cacaagcttc aacacaagtt ttctttcttg
     1201 tcgttttttc attctactca caaatctact cacaatattt tttgttgccc ctgattttcg
     1261 atactttacg tcgtctgagt tatcggattt gaaatccgat tggttttccc cgcctgatac
     1321 ccatggttca cttgacccgg tgcaacggtt gcactatatt gaaaacatga gaacgattca
     1381 ccgtcaggca gggtggaggg tcgccatcgc ggcgagagac cacggaatac cacactttca
     1441 cgtcatattc agcgacggaa ccagttgtgc cgtttcgata gacacgctca aggtgcttgc
     1501 cggagccgtt cttccccgga aggttgcccc ggcgattgag tgggccgaag atcagaaagc
     1561 cactttgctg aaggtttgga aggagatgaa cccatgaagg aaatgcgaat taccgccgtt
     1621 gaggttgttt ctctcggtgt cttgcggata acttggaacg acacagaaac ctatcaggt
//
LOCUS       DAORHD010001210         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95957_length_1678_cov_4.027110, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001210 DAORHD010000000
VERSION     DAORHD010001210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95957_length_1678_cov_4.027110"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKO06_20175"
     CDS             complement(<1..238)
                     /locus_tag="PKO06_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72034.1"
                     /translation="MKRLFISTLLLSTLSFPVSAGPSPDPFLKQVKELMQAKNYDRAI
                     TLINTELRAKPQEYQLWLAMGYCYEGLQKSKEALD"
     gene            complement(297..>1678)
                     /locus_tag="PKO06_20180"
     CDS             complement(297..>1678)
                     /locus_tag="PKO06_20180"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72035.1"
                     /translation="EVVSMLNEYLDAMTEVIRRHHGVVDKFIGDAVMAVFYPLETRDD
                     EKRAVLCGLEMMSELKRFNRRREEVGLFPVGMGVGISSGLVVAGKIGSKNGRIDYTVI
                     GDTVNVAARLEGVSKIGKHTRVVVSDATWQAVKEMFEAEPMGIDRVKGKARQVQMFEI
                     IGPRHVDQFLQDLNSDEEPRRLAAVQALGLSGQAQWIEQLIPLLRDANPRVRSSTAQA
                     LKNLISHDQRIVFHLKEALAGETDSFVIASFILDIGLLGSDSDRLQLVQFLDHDDNRI
                     RANTVEALSYMHDRRRLQSLLRPKLSDSNNRTRANAAMLLYQTGELDGLRTLLDMCTE
                     KSSALMRASGAYGIGEVTSRERAREILQSIDHPETIVSVDRLQLLSQARLQLEALLQD
                     HDQMVRKNAARALGRMGHEGSVPALVRCFRSLSAEDAARPTVLSAVTEISGSHVMKVL
                     ENELTTPSS"
BASE COUNT          355 a          482 c          462 g          379 t
ORIGIN      
        1 catcgagtgc ttcctttgat ttctgcagac cttcgtagca ataccccatc gccagccaca
       61 gttgatactc ttgcggtttg gcacgtaact cggtattgat caaggtgatt gcccgatcat
      121 agttcttggc ttgcatcagt tctttcactt gtttgagaaa cgggtcggga gaggggcccg
      181 cagacactgg aaagctgaga gtgctcagaa gcagagtgct gatgaaaagg cgtttcatgg
      241 ttatcctcct gacagacaga cttctggcgg tagtgtatca caccacgacg aaaccttcag
      301 gagctcggtg ttgtcagctc gttttcgagc actttcatca catggcttcc agagatctcg
      361 gtaaccgcag aaagaaccgt gggccgcgcg gcgtcttctg cgctcaacga acgaaagcat
      421 ctgacgagtg ccggaaccga gccttcgtgc cccatacgac cgagcgcgcg tgccgcattt
      481 ttccgcacca tctggtcgtg atcctgcagc agagcttcga gttgcagacg agcctgtgaa
      541 agcagttgca ggcgatcaac cgagacgatg gtctcgggat gatcgatgct ctgcagaatc
      601 tcccgggcac gctcgcgcga cgtcacttct ccgattccat atgcgccaga agcgcgcatg
      661 agcgccgaac tcttctcggt acacatgtcg agcagagtcc gcaggccgtc cagttcgccg
      721 gtttggtaga gcagcatggc ggcattcgcc cgggtacgat tgtttgaatc tgacagtttc
      781 ggacgcagca ggctctgcaa tcgccggcgg tcgtgcatgt agctgagcgc ttcaacggtg
      841 ttcgcacgga tgcggttgtc gtcatggtcg agaaactgta ccagctgtaa acggtcagaa
      901 tcgctgccca agaggccgat atcaagaatg aaagacgcaa tgacaaagct gtccgtttct
      961 ccggcaagtg cctccttcag gtgaaacacg atgcgctggt catgactgat caggtttttc
     1021 agagcctggg ccgtcgagct cctgacgcgc gggttcgcgt cacgcagcaa cggaatcagt
     1081 tgctcgatcc attgagcctg acctgagagg ccgagagctt gtacggcggc gagacgacgt
     1141 ggttcctcgt ctgagttcag atcctgcagg aattggtcga catgccgtgg accgatgatc
     1201 tcgaacattt gtacctgtcg agccttgccc ttcacccgat caattcccat gggttcggcc
     1261 tcgaacatct ccttcactgc ctgccacgtg gcatctgaga caacgacgcg ggtatgcttg
     1321 ccgatcttgc tgacaccttc gaggcgcgcg gcaacgttca ccgtatcacc aatgaccgtg
     1381 taatcgatgc gtccgttctt ggaaccgatc tttcctgcga caaccaggcc gctgctgatg
     1441 ccgacaccca ttccgaccgg aaacagaccg acttcttcgc gtcgtcgatt gaagcgcttg
     1501 agctcactca tcatctcgag gccgcacagc acggcgcgct tctcgtcatc gcgggtctcg
     1561 agtggataga aaaccgccat gaccgcgtca ccgataaact tgtcgacgac accatgatga
     1621 cgtcgtatga cctcggtcat ggcatcgaga tactcgttga gcatcgagac aacttcct
//
LOCUS       DAORHD010001211         1678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_95965_length_1678_cov_3.639556, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001211 DAORHD010000000
VERSION     DAORHD010001211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95965_length_1678_cov_3.639556"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            304..855
                     /locus_tag="PKO06_20185"
     CDS             304..855
                     /locus_tag="PKO06_20185"
                     /inference="COORDINATES: protein motif:HMM:NF020590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HNV72036.1"
                     /translation="MTLNPPVVSIDNRKRPSYNDSILTEIRPEVIMNQAHFESLLYQE
                     GKISLSKAALLCSMIGNEFDDELVAFLAQKQIRSVITRAGGSGDNLKSKILRNAFGAA
                     EKSGLLMPTPRNRYGITLLVEELMRAFDSPLLDVAGGGLKIGLAVQKHDLAMGMSGKL
                     GIPGMDVDYEVSMARTLYHYLED"
     gene            858..1271
                     /locus_tag="PKO06_20190"
     CDS             858..1271
                     /locus_tag="PKO06_20190"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV72037.1"
                     /translation="MIGIVVITHGKLSEELKETARLIMGDQNDFHTVTFTARESLDAL
                     RAKAREAIDRFRDSGCLVLTDVLGGSATNVCVELLQEDWIRVITGVNLPMVMEALQNR
                     QTLDLPNLSHKVRDGAARAIIDLKEFYQQRMKKKA"
     gene            1268..>1678
                     /locus_tag="PKO06_20195"
     CDS             1268..>1678
                     /locus_tag="PKO06_20195"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HNV72038.1"
                     /translation="MTRPIRFLRIDDRLVHGQVIVGWLPHLQVSHLLVINDKTAADPM
                     RQDMMSLSVPGSVKLLFAAPTAMAQAPEFPVQTLAVVGSPRDAWAAIQSGLAPEVLNV
                     GGLHSRAGREEIREALHLDADDRDHLQKFIDAGIM"
BASE COUNT          366 a          488 c          473 g          351 t
ORIGIN      
        1 ggtggcgaat gcgattcgtc agcggaatgt gcgggcgaag atcctgctgc gcagtggcta
       61 tagtcgcgag ttggtcgatc agacgttcct gcagccaggt accatcgagt ttctcgaaaa
      121 accgtcaacc tctgacatcc ttctcgagcg cattcgcaca ctcctcgatt cgcgatgacc
      181 ctccgttcgc gtgagcgccg tcaatcaggt ttcagcctgc gtttcgagat gaagggcgca
      241 ctccgtgcca tgacggcgaa tcgttgagcg ataccaaggg gaaaacccaa gcccgagaat
      301 caaatgaccc tgaacccgcc tgtcgtttcc attgacaacc ggaaacgccc ttcctataat
      361 gattcgattc tgacggagat cagaccagag gtcatcatga accaggcgca ttttgaaagc
      421 cttctctacc aggagggcaa aatctcgttg tcgaaggcgg cactgctgtg cagcatgatc
      481 gggaacgagt tcgacgatga actggtggca tttctggccc agaaacagat ccgctccgtc
      541 attacccgcg ccgggggcag cggcgacaac ctgaagagca agattctacg caatgccttc
      601 ggagccgccg aaaaaagtgg cctgctgatg ccgaccccac gcaatcgcta cggtatcacc
      661 ctgctggtcg aagaactgat gcgggcgttc gatagtccac tgctcgatgt cgcgggcgga
      721 gggctcaaga tcggcctggc ggttcagaaa catgatctgg ccatgggaat gtcgggaaaa
      781 ctcggcattc cgggcatgga cgtcgattat gaggtttcca tggcccgcac gctctatcac
      841 tatctcgagg attagtcatg atcggcatag tcgtcatcac ccacggaaaa ctctctgaag
      901 aattgaaaga aacggctcgc ctgatcatgg gcgatcaaaa cgatttccat accgtgacct
      961 tcaccgcgcg cgaatctctc gatgccttgc gtgccaaggc gcgcgaagcg atcgatcgct
     1021 ttcgtgactc ggggtgcctg gttctcaccg atgtcctcgg cggcagcgcg accaacgtct
     1081 gtgtcgaact cctccaggaa gactggatcc gcgtcatcac cggagtgaat ctgccgatgg
     1141 tcatggaagc gttgcagaac cggcagacgc tcgatctgcc caatctttca cacaaggtgc
     1201 gcgatggggc ggcccgcgcg atcatcgacc tgaaagaatt ctatcagcag aggatgaaaa
     1261 agaaagcatg acccgtccga ttcgctttct gcgcattgat gaccgtctgg tacatggtca
     1321 ggtcatcgtt ggctggttac cacatctgca ggtttcacat ctgctggtga tcaacgacaa
     1381 gacggccgct gatcccatgc gccaggatat gatgagcctc tccgttccgg gttcggtcaa
     1441 actgctgttt gccgctccca cggcgatggc tcaggcccct gagtttcccg tccagaccct
     1501 ggcggtcgtg ggctccccgc gcgatgcctg ggccgcgatt cagtccggac tggctcccga
     1561 agtactcaat gtcggcggtc tccattcccg ggccgggcgc gaagaaatcc gcgaagccct
     1621 gcatctcgat gcggatgatc gcgaccattt gcagaaattc atcgacgcgg gcataatg
//
LOCUS       DAORHD010001212         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_96186_length_1676_cov_2.865515, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001212 DAORHD010000000
VERSION     DAORHD010001212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96186_length_1676_cov_2.865515"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKO06_20200"
     CDS             complement(<1..500)
                     /locus_tag="PKO06_20200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72039.1"
                     /translation="MTCERLSDWLAGDSPACRELPSDLRQHVDVCPECRAVWQGVQNL
                     RREAETLALSGPERVSLWNDLQRRRQAAMPQTAPQGSPASLQSRPSSQIQPGAPSLNV
                     LPSADWSSWLSNWLAPALAFGVVLLVGAWFGLRDSGPLVMPPTDRPHTVTAPTVRQPP
                     AWAMLQG"
     gene            complement(478..1026)
                     /locus_tag="PKO06_20205"
     CDS             complement(478..1026)
                     /locus_tag="PKO06_20205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV72040.1"
                     /translation="MNPTDDVLMERWQKGDEQAFHDLYDRYLPLVVRYIMTLCGNWEE
                     AKDLAQETFMRVCERKESFQSRSRFKSWLLTIARNKVIDATRKRSVKTVVSEELLARA
                     PAPEEGFPCETTLDTEQMPGFKALTAGQREVVLLRFVEDLSFPEIAAVTSMREDNIRK
                     ILSRALQRLRKEWQTHDVRTAE"
     gene            complement(1288..>1676)
                     /locus_tag="PKO06_20210"
     CDS             complement(1288..>1676)
                     /locus_tag="PKO06_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72041.1"
                     /translation="AKPEPNKRGLYYQPTRVDALVEHLKKGNFGDVALKNISAKTVFG
                     EWMHDISIGSRTFSPFEFYPDGLTASDAVKLAQAKEKGSYPTGFEGHDLKAVVFANFL
                     FIYDPRDEHSALWEKVTEFCKTFSVP"
BASE COUNT          351 a          516 c          455 g          354 t
ORIGIN      
        1 ccctgaagca tagcccacgc cgggggctgg cgcacggttg gggcggtcac cgtgtggggg
       61 cgatccgtcg gtggcatgac gagagggcct gaatctcgca gcccaaacca ggcgcccacc
      121 agcaggacaa ccccgaacgc cagagccgga gccagccagt tcgacaacca ggaactccag
      181 tcagcgctcg gaagcacgtt caaagaaggc gcgcccggct gtatctgcga cgaaggccga
      241 gattgaagcg aggcaggaga cccctgcggt gcggtctgcg gcatcgcggc ctgccgccgt
      301 cgctgcaggt cgttccacag tgaaacccgc tcgggtcctg acagagccaa ggtttcggct
      361 tctcgccgca ggttctgaac cccctgccag accgctcggc attccgggca gacgtcaacg
      421 tgctgccgca gatcagaggg caactcacgg caggcgggcg aatccccggc aagccagtca
      481 ctcagccgtt cgcacgtcat gggtctgcca ctcctttcgc agtctctgca gagcacgaga
      541 gaggattttg cgaatattgt cttctcgcat cgatgtcacc gcggcaattt ccggaaatga
      601 aaggtcttcg acgaatcgca acagaacgac ttcgcgctgt ccggctgtca aagccttgaa
      661 cccgggcatc tgctcggtgt cgagcgtcgt ctcgcacggg aacccctctt caggagccgg
      721 tgcccgcgcc aacagctcct cagacaccac cgttttcacg ctgcgtttgc gggtcgcatc
      781 gatgaccttg ttgcgcgcaa tggtcagcag ccagcttttg aaacggctgc gactctgaaa
      841 ggattctttt cgttcacaga cgcgcatgaa ggtctcctgg gccaggtctt tggcttcttc
      901 ccagttcccg cacaaggtca tgatgtatcg caccaccagt ggcagatacc gatcatagag
      961 gtcgtggaac gcctgttcat cgcctttctg ccagcgttcc atcaagacat cgtcagtcgg
     1021 attcatgctt cctgatgtaa ggttcaaaca gcggtttccc tgggtttcat tctctataac
     1081 ggaagaccct ctcgatttgt gacatcttct tcccggtatc gggttccctg tggcgagcga
     1141 ttccgcaaca cctctattct acccgcccga acacgaacaa acaacggaag agtgccgcac
     1201 atcgcaggct tttcaatctc cgaagatgac acgtggcctg aagacgaaat gcttcaggcc
     1261 acgcatgcgg tctcacggtt cgtttgatca gggaacgctg aacgttttgc agaactcggt
     1321 caccttctcc cacagcgcgc tatgttcgtc tcgcgggtca tagatgaaca ggaaattggc
     1381 aaacaccaca gctttcaggt catgtccctc aaaccccgta ggataactcc ccttttcctt
     1441 tgcttgagct agtttcacgg catccgaggc ggtcaaacca tcgggataga attcgaaagg
     1501 cgagaaagtg cgggagccaa tcgaaatatc gtgcatccac tctccaaaga ctgtcttggc
     1561 gctgatgttc ttcagcgcga cgtctccgaa gttccccttc ttcaaatgct caaccagcgc
     1621 atcaacgcgt gtcggctgat agtacaagcc ccgcttgttc ggttcgggtt tggcca
//
LOCUS       DAORHD010001213         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_96343_length_1674_cov_4.222977, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001213 DAORHD010000000
VERSION     DAORHD010001213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96343_length_1674_cov_4.222977"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="PKO06_20215"
     CDS             complement(<1..1016)
                     /locus_tag="PKO06_20215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72042.1"
                     /translation="MSRSRRPSLPSSQTGHGQRESPRQVAPKRPTEDRGISGQLLSGF
                     CRLVDHAPIMVWVMDHLGNTVFLNRLFLEFTGLAANVSPIPSWDVSVHDEDAPALRRT
                     FSVAAAAGLPLAFECRLRRHDGIWRHVQVHARPFPGESGQTTGYLGFCFDVHERFETR
                     EALVQSDARFRSLVEQIPAVLYMAPVENVARLEFVSPQVEQLLGYTAADFLHQPDLWR
                     SCIHPDDLDRIEFRPKLTGMANRAFVAEYRMVSRDGTVRWIHDEAVMVHRPDGRPVCY
                     QGIMLDVTERRQLEETLRIYAEAIEGLDDLVAAIDRRQRFIMVNRSWLEYQGKTREEV
                     MGKHL"
     gene            complement(1029..>1674)
                     /locus_tag="PKO06_20220"
     CDS             complement(1029..>1674)
                     /locus_tag="PKO06_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNV72043.1"
                     /translation="TDRERKRRAGLIVPSYVAYVLEGYGRTLAQFPELNSYLRVYPFT
                     RLAVYSGVDISLTVEREWEGRRIVLLAILREAQNKSAEEIHEFLRARRECPLDELEEF
                     ARYKKLLKVPAFCRWHLFQLCIKPFPGLMRDLVGTTAFTSIGKFGSTFTTPLSPRTVT
                     LSLGRVEPRPRVVGNDVRPALSAWVTVTYDHRVSDGAEVARFGDELRRYLERPL"
BASE COUNT          359 a          540 c          467 g          308 t
ORIGIN      
        1 aggtgtttcc ccatgacctc ttctcgagtc ttgccctgat actcgagcca cgatcggttc
       61 accatgatga atcgttgacg acggtcaatg gccgccacga gatcgtccag gccctcgatc
      121 gcctcggcat agatacgaag cgtttcttcg agctgccgtc tctctgtgac gtcgagcatg
      181 atcccctgat aacacaccgg acggccatcg gggcgatgca ccatgaccgc ctcatcatga
      241 atccagcgaa cggtgccatc acgcgacacc atgcgatact cggcgacgaa ggctcgattc
      301 gccattcccg tcagtttcgg ccggaactcg atacgatcga gatcatccgg atggatgcaa
      361 ctgcgccaca gatcaggctg atgcagaaag tccgcggcgg tatatcccag cagttgttcc
      421 acctgcgggc tgacaaattc cagacgagcc acattttcca ccggcgccat gtacaatacc
      481 gccggaatct gctctaccag cgagcggaac cgggcatctg actggacaag tgcctcacgg
      541 gtttcaaatc tctcgtgcac gtcaaagcag aatcccagat agcctgtcgt ctgccccgat
      601 tcaccgggaa acggacgcgc atgaacttgt acatgtcgcc agatcccgtc atgccgtcgc
      661 agtcggcact cgaacgccag cggcaggccg gccgcagcgg ccacactgaa cgtgcggcgc
      721 aacgccggag catcttcatc atggaccgac acatcccagg acgggatggg tgacacgttc
      781 gcggccaatc cggtgaattc gagaaacagc cgattgagaa agacggtgtt ccccaggtgg
      841 tccatgaccc acaccatgat cggcgcatgg tcaaccagcc ggcagaaccc cgacagcaat
      901 tggccggaga tcccgcgatc ctctgtcggc ctcttcggcg ctacctgccg cggagattca
      961 cgctgaccat gccccgtctg ggacgaaggc aaagaggggc gccgacttcg actcatgaag
     1021 atctcgcgtt acaacggtcg ttcgagatag cgccgcagtt cgtcgccgaa ccgggcgact
     1081 tcggctccat cgctgactcg atgatcatac gtgaccgtca cccacgccga caacgccggt
     1141 cgcacatcgt tgccgacgac cctcggtcgc ggctcgacgc gacccagcga cagcgtcacc
     1201 gtacgtggcg ataacggcgt ggtgaacgta ctgccaaact tgccgatgct cgtgaaggcg
     1261 gtcgttccga ccaggtcacg catcagtccg gggaaaggct tgatgcaaag ctgaaacaga
     1321 tgccagcggc agaacgcggg gaccttcagg agtttcttgt atcgggcgaa ttcctcgagt
     1381 tcgtcaagcg ggcactcacg ccgcgcccgc aggaattcgt gaatctcttc ggcactcttg
     1441 ttctgcgctt cgcgtaaaat ggccagcagc acgatgcggc ggccctccca ttcccgttcg
     1501 acggtcagcg agatgtcgac gcccgagtac acggccagcc tggtaaacgg gtagacgcgc
     1561 agataactgt tcagttcggg aaactgcgcc agcgttcttc catacccctc gagaacgtat
     1621 gccacatagg aaggcacgat caaccccgca cgccgcttgc gttcgcgatc ggtc
//
LOCUS       DAORHD010001214         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_96361_length_1674_cov_3.126004, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001214 DAORHD010000000
VERSION     DAORHD010001214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96361_length_1674_cov_3.126004"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKO06_20225"
     CDS             <1..895
                     /locus_tag="PKO06_20225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV72044.1"
                     /translation="ISILIQGESGTGKELVAEAIQRQSSRASGPFIKINCAALPENLI
                     ESELFGYEKGAFTGAAGRKRGKFEVANGGTLFLDEIGDMSLQTQAKVLRAIQEREIER
                     LGGTDPIKIDVRLITATHKDLKSLISKGQFREDLFYRINVVTIQLAPLRERVEDLVAL
                     IEHFVGVVSQKLGRSIKGVSPRALTALKNFKWPGNIRQLQNIIEGAAVFAKGEYIDIE
                     NLPEEIRLGFAPESAVSPVDWVAGEIEKGKTFDEVIGQIEKEIIMRTLAATAGNQSQA
                     AAKLGMKRGTLQYKIKQYEIV"
     gene            complement(991..>1674)
                     /locus_tag="PKO06_20230"
     CDS             complement(991..>1674)
                     /locus_tag="PKO06_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72045.1"
                     /translation="IMAHELQHMMYFNQKGITGSTWIDEGLSVWAQQLVGEGFPQGRT
                     TPVQQVGAYLKAPNTVSLNHWADNSLANYGMSYLFVQYLFERCGQFSAIQKLERNTGP
                     TGFNDINNVLVDATSTTQLKPFLQDDFGLAMYCDGLDLPTALTGYVPAVHQFSGFQLR
                     KGISGINGLSRISLNENPVTNQVFEMLGYGFDVVEYARGNGGDVEWTLVSSPSADFHV
                     WVLYYQTDD"
BASE COUNT          386 a          441 c          500 g          347 t
ORIGIN      
        1 gatcagcatt ctcattcaag gtgaatcagg taccggcaag gaactggtgg cggaagccat
       61 tcagcgacag agcagtcgtg cttctgggcc gttcatcaag attaactgcg ccgctttgcc
      121 cgagaatctc atcgaaagcg agctgttcgg ttacgaaaaa ggcgcattca cgggggcggc
      181 cggccgcaaa cggggcaagt tcgaggtggc gaacggtggt acgttgttcc tcgatgaaat
      241 cggcgacatg agtctgcaga cccaggccaa ggttctgcgc gccattcagg aacgcgagat
      301 cgaacgcctg ggcggcaccg acccgatcaa gatcgacgtg cgcctgatca ccgccaccca
      361 caaagatctc aaatcgctga tctccaaggg acagtttcgc gaagatctgt tctatcgcat
      421 caatgtggtc acgattcaac tggccccgtt gcgggaacgg gtcgaagatc tggtcgccct
      481 gatcgaacat tttgtcggcg tggtgtcgca gaagctcgga cgctccatca agggggtctc
      541 tccgcgagcc ttgacggcgt tgaaaaattt caagtggccg ggaaacattc gtcagctgca
      601 gaacatcatc gaaggggccg cggtcttcgc caaaggagaa tacatcgata tcgagaacct
      661 gccggaggag atcagactcg gcttcgcgcc cgagtcggcg gtttcaccgg tagactgggt
      721 tgctggagag atcgagaagg gcaagacctt cgatgaggtg atcgggcaga tcgagaaaga
      781 gatcatcatg cgcacgctgg cggcgacggc cggcaatcag tcacaggccg ctgcaaagct
      841 gggtatgaag cgcggcacgc ttcagtacaa gattaaacag tacgagattg tttgacgaag
      901 cctcgtataa ggctcaataa gcgctgagtc gcctcgagat cgtgcgaggt gggttcgccc
      961 tgttggactc gaatactggt cgatgaaagt tcaatcgtcg gtctggtaat agagaaccca
     1021 gacatgaaaa tcggccgaag ggctgctgac gagcgtccac tcaacgtcac cgccatttcc
     1081 gcgagcatac tcgacgacgt cgaaaccgta tcccagcatc tcgaacacct ggttggtgac
     1141 gggattctcg ttcagcgata tccgcgaaag cccattgatg cctgatatcc ccttgcgcag
     1201 ttgaaagccg ctgaactggt gaacggcagg gacgtaaccc gtgagagcgg ttggcagatc
     1261 aagtccgtcg caatacatgg ccagtccgaa atcatcctgc aggaagggct tcagttgcgt
     1321 cgttgatgtt gcatcaacca ggacattgtt gatgtcattg aagccggtcg ggccggtgtt
     1381 cctctcgagt ttctgaatgg cgctgaactg gccgcagcgc tcgaacagat actgcacgaa
     1441 gaggtaggac attccgtagt tggcgagact gttgtcagcc cagtggttca aggagacggt
     1501 gttcggcgct ttcagatagg ctcccacctg ctgaaccggg gtcgttcgac cctgtggaaa
     1561 tccttcgccc accagttgct gggcccagac cgagagtccc tcgtcgatcc aggtcgagcc
     1621 ggtaatgccc ttctgattga aatacatcat gtgctgtagt tcgtgtgcca tgat
//
LOCUS       DAORHD010001215         1669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_96877_length_1669_cov_2.703841, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001215 DAORHD010000000
VERSION     DAORHD010001215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96877_length_1669_cov_2.703841"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="PKO06_20235"
     CDS             complement(<1..672)
                     /locus_tag="PKO06_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72046.1"
                     /translation="MIRQVCLVFLFCLLIFYPAHGYAQALDEAAAAPAASASESTPAA
                     PEPTSAPVAKGGGGWLQNWVTGSAEFVRVLEGILEQLARLFGIPVPFPAPQAEPGVPT
                     ADTGSSSPVAQPGTSDSGPTSGTTAPSDTSGVASSATGPITNPGVTSGGDGGTIAIPP
                     RPADARGGRAFIESTNRMTRPQREQAVFDEISRGNVPEFVRTWKTIEATFTGRDGQTH
                     TVRYRV"
     gene            complement(719..>1669)
                     /locus_tag="PKO06_20240"
     CDS             complement(719..>1669)
                     /locus_tag="PKO06_20240"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72047.1"
                     /translation="GPLLMCAVPGRVLTKGVFCGRKVLSAVRPRLAEQERKFYVAMLI
                     SIFLATLAALVGSQFVTSPLSAILSAIQQINREEYEARIRFPERGDEFASLGRAFNDM
                     AQGLQEGRILARYVSTTVQEAVSDSTLNERATEGEMTEVTVLFTGLEGFDDYRRLHTP
                     DEVFALLAINLESANAAVSACGGEIDKVMGEKILILFRHDRFLSGAQAMHAALKVIRH
                     MRDDLQKRGIHPLFGLNSGTVVAGFVGAQNVRMDYTVIGDPVNLAARLVALAQSLGGS
                     GVITSGLAVRLMSPEIVAEKLDITRVKGKTQEVEAYRLQL"
BASE COUNT          350 a          484 c          512 g          323 t
ORIGIN      
        1 gacacgataa cgcacggtat gggtctgacc gtcgcgaccg gtgaacgtgg cctctatcgt
       61 cttccaggtg cgtacaaatt cggggacatt gccacgggag atctcgtcaa aaaccgcttg
      121 ctctcgctgg ggccgcgtca ttcgattggt actttcgata aaggcccgac ccccgcgtgc
      181 atcggcaggc cgaggaggaa tcgcaatcgt tccaccatcg ccgccgctgg tgaccccggg
      241 attcgtgatt ggccctgtgg cggaactggc cactccagag gtgtcggaag gggcggtcgt
      301 ccctgacgtg gggccggaat ctgatgttcc cggttgtgcg acaggagacg aagagcccgt
      361 gtcagccgtg ggaacgccgg gctcggcctg tggcgctgga aacggaactg ggatgccaaa
      421 gagacgggcg agttgttcga ggatgccctc gagcaccctg acgaactcgg cggagcctgt
      481 gacccagttc tgtaaccaac ctccaccgcc tttggcgacc ggggcagacg tgggttcagg
      541 agcggcgggt gtgctttctg atgctgaggc cgcgggagcg gcggcagctt catcaagggc
      601 ctgcgcatac ccgtgagcag gatagaaaat cagcagacag aagaggaaaa cgagacagac
      661 ctgtcggatc atgacgaacc tccttggtga acgtgaatct ctggtgtgta caagcgaatc
      721 agagctgcaa tcggtaggct tccacctcct gggttttccc tttgacccgg gtgatatcca
      781 gcttctcagc cacgatctcg ggagacatca aacgtacggc cagccctgag gtgatcactc
      841 ccgaacctcc cagactctgg gccagagcga ccagacgtgc cgccagattc acgggatctc
      901 cgatcaccgt gtaatccatg cgcacgttct gggcgccgac gaatccggcg acgaccgttc
      961 cggagttgag cccgaacagg gggtggatac cccgcttctg gaggtcgtca cgcatgtggc
     1021 gaatgacctt gagagcggca tgcattgcct gtgcgccact gagaaaccga tcgtgtcgaa
     1081 acagaatgag gatcttttcc cccatgacct tgtcgatctc tcctccgcag gcactgacgg
     1141 cggcattcgc ggattcgagg ttgatcgcca gaagagcgaa gacttcatcg ggggtatgca
     1201 gacgacggta gtcatcgaat ccctcgagac cggtaaacaa cacggtcact tctgtcatct
     1261 caccctcggt ggctcgctcg ttgagcgtgc tgtcgctgac agcctcctgg acggtcgtgg
     1321 aaacataccg cgcgagaatg cgtccttcct gcagaccctg tgccatatcg ttgaacgcgc
     1381 gtccgagtga ggcaaactcg tcacctcgtt ctggaaagcg aatgcgggcc tcatactctt
     1441 cgcgattgat ctgttgaatg gcgctgagaa tggccgacag ggggctggta acgaattgac
     1501 tgccgaccag cgctgccagg gtggccagaa aaatcgagat cagcatggcg acatagaact
     1561 tgcgttcctg ctcggcaaga cgcggtctta ctgcgctcaa gactttccgt ccacagaata
     1621 cccctttggt cagaacacgg ccagggacgg cacacatcag cagagggcc
//
LOCUS       DAORHD010001216         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_96974_length_1668_cov_2.719157, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001216 DAORHD010000000
VERSION     DAORHD010001216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96974_length_1668_cov_2.719157"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            332..832
                     /locus_tag="PKO06_20245"
     CDS             332..832
                     /locus_tag="PKO06_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72048.1"
                     /translation="MGRSSMRGTTFLEILISMALLFLLAMTITRYFSETSLPQQMMIR
                     QFPVAMHLAEKTLNVLANEISGGKTPLPPEGETDLTASMLEHPQFLEELRVLWPGLNL
                     NRSEISVPLRAALQVRYLAGLPMADVSGTTLPGVDGRIVNLTLHLSWGHEPSHHLYHC
                     LSVPQP"
     gene            829..1335
                     /locus_tag="PKO06_20250"
     CDS             829..1335
                     /locus_tag="PKO06_20250"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72049.1"
                     /translation="MRWFFSKRPPGGFTLVELLIGTGLFLVFLGVAFGTYTNYRTWFQ
                     ASTTRLDVGHRLRLSGQRLDEMLTDCRKILYPPRGAASATRLLYLDSRNQLQAVSLDV
                     TGLLTQIDVLQAIKGEPESARVLGGPLSLFQVEHVTLRQLSYRMTATPGKGKSETLQQ
                     LLQLWGAE"
     gene            1335..>1668
                     /locus_tag="PKO06_20255"
     CDS             1335..>1668
                     /locus_tag="PKO06_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72050.1"
                     /translation="MSNRHGFSLSLLVVIGGVLFILLLALSHYSSQEIRMVKKLIARR
                     KAEAAAISGMNWVVARLKQPQAGRWYQPPRTRTGSGGAGTTWLWEHRVTPALFPAESG
                     MSVEISLDE"
BASE COUNT          359 a          467 c          463 g          379 t
ORIGIN      
        1 atgcattcag aaaacgatag taagcttcga gcacttcccc tctgatcaca ttgtcttcga
       61 gcttgttgtc gatacgatcg attcttgcgt accgaccaaa aaagagtgtg atgcagatca
      121 ccagagcgag aaccgcccat gcccaacgtg ggcgattcag cagttccgcc agcgtcacgc
      181 gcgtggtttc ctttcttcag aaggtgatca ggagagggtg tcgcagtata ccacagtcgg
      241 tgcacgaagg ccgtcagcag agttctcctg agggcaaatc ggtctcgggc acgcctgtga
      301 tacaatcgac agaagcgggg aattgaccga tatggggcgt tcatcgatgc gcggaaccac
      361 ttttctcgag attctcattt ccatggccct gctctttcta ctcgccatga ccatcacccg
      421 gtatttctcc gagaccagtc tgccgcaaca gatgatgatc agacaattcc ccgtggccat
      481 gcatctggcc gagaaaacac tcaacgttct tgccaatgag atctctggcg ggaaaacacc
      541 gctcccgcca gagggggaaa ccgatctgac ggcatcaatg ctggagcacc cacagtttct
      601 ggaagagctt cgtgtgctgt ggcccggcct caatctcaac agatcggaga tctctgttcc
      661 cctgcgggcg gcccttcagg tacgttatct ggcagggctc ccgatggcag atgtttcggg
      721 aacaaccctg ccaggggttg atggtcgcat cgttaacctg accctgcatt tgtcctgggg
      781 tcacgagccc tctcatcatc tctatcactg tctttcggta cctcagccat gagatggttc
      841 ttctcgaaac gaccacccgg tggcttcacg cttgttgaac tgctgatcgg cactggtctg
      901 tttctcgtgt ttctgggagt ggcctttgga acgtacacca actaccgaac gtggtttcaa
      961 gcctcaacga cgcgcctgga tgtcggacac cgtctgcgat tgagcggtca gcgtctcgat
     1021 gagatgttga cggattgccg gaagatcctg tatccaccgc gaggtgcagc ctccgcaaca
     1081 cgtctgctct atctcgactc aagaaatcaa ctgcaggccg tgtctcttga tgttaccgga
     1141 ctcttgaccc agatcgatgt gctccaggca atcaaaggtg aacccgagag cgccagggtc
     1201 cttggtggcc ccctgtctct gtttcaagtt gaacacgtca cgttgcgaca gctttcatac
     1261 cgaatgaccg ctacgcccgg aaaagggaag agcgaaacgt tgcagcagct gttgcagcta
     1321 tggggtgcgg agtgatgagc aacagacacg gcttttcctt gtccctgctg gtcgtgatcg
     1381 ggggcgtgct gtttattctt cttctggcat tgtctcacta ttcgagccag gaaatccgga
     1441 tggtgaagaa gctcatcgcc cgaagaaagg cagaagcagc ggcaatctcc ggcatgaact
     1501 gggtggtcgc aagactcaaa caaccacagg cagggcgttg gtaccagccg ccacgcaccc
     1561 gcacagggtc tggcggggca ggaacgacgt ggctgtggga gcatcgagtc acgccagcgc
     1621 tgtttccggc agagtccgga atgagtgtcg agatctctct tgatgagc
//
LOCUS       DAORHD010001217         1667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_97051_length_1667_cov_3.083747, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001217 DAORHD010000000
VERSION     DAORHD010001217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97051_length_1667_cov_3.083747"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1605)
                     /locus_tag="PKO06_20260"
     CDS             complement(7..1605)
                     /locus_tag="PKO06_20260"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HNV72051.1"
                     /translation="MQRNPPFRPTSAIAILILGLFLSTAVFGQPSTTPYNDHSVTAPS
                     VSGSSSVTASGTPTDDVILTDEERELITMKLLPRYYPMLKQAMEKPFGSLERAKAKAR
                     SLYRYPNAVYSPRFGDPVHMLEWARRYRGDAAMVKFCASTVRGDIILTGHRTEEGQKN
                     DMIAILTGGKYHHAVTVIDGPPCVFIEAVGLTGTKSDPTNNRVRLASWHEQLASWAGM
                     RLLRPTAGLPTAQANRYIEGAVQYLVDQLGKPYDYGFTNNDVNRAFYCSELAWKSYYE
                     GGGMKSFKPVKSSERDRMVVALNAVVDGLRPKDRVALANRVMTFTGEYLAQKPPDLKK
                     LNNFIVDEIVPGCGLCSDAFPTPEAREKLRTVLEKVRNNQAFTSYLKAREDYLAAEKA
                     GKFKTGWGIGAARKLAAEMKIAGSIAADINAMVKEAGAGYAKTVKLVSGLIGPIYKHL
                     GTYTDFMTGMNKEGKMQLPDGAKTVLSITDWLAKKREQVKNWPIGSSLANLLPGYGDD
                     KVQTSFTSPTDLANTSPNFHLDYP"
BASE COUNT          319 a          477 c          475 g          396 t
ORIGIN      
        1 gctgtctcaa ggataatcga gatggaagtt cggtgaggtg ttggccagat cggtcgggct
       61 ggtaaatgag gtctgcacct tgtcatcacc gtatccgggt aacagattgg ccagactcga
      121 accgatcggc cagttcttga cctgttcgcg cttcttcgcc agccagtcgg tgatcgaaag
      181 caccgttttg gcaccatcag gcagctgcat ctttccttcc ttgttcatgc cggtcatgaa
      241 gtcggtgtag gtgcccagat gtttatagat cggcccgatc agaccgctca ccagtttgac
      301 ggttttggca tagcctgcac cggcttcttt caccatcgcg ttgatgtcgg cggcaatcga
      361 cccggcgatt ttcatctcgg cggccagttt gcgggcggcc ccaatacccc atccggtttt
      421 gaacttcccg gccttttccg cggccagata gtcttcgcgg gctttcagat acgaggtgaa
      481 cgcctgattg ttgcgcactt tctcgagcac ggttctcagc ttctcgcggg cctcaggcgt
      541 cggaaaggcg tcagaacaga gcccgcaccc cggaacgatc tcgtcgacga tgaagttatt
      601 gagtttcttc agatcgggcg gtttctgagc gagatactcg ccggtgaacg tcatgacccg
      661 attggcaagc gcgacccgat ccttgggacg taatccatca accacggcat tcaacgcgac
      721 caccatgcgg tcgcgctcgg acgatttcac cggcttgaaa ctcttcatgc cgccgccttc
      781 gtagtagctc ttccaggcca gttcactgca gtagaaggct cggttcacgt cgttattggt
      841 gaagccataa tcgtatggtt tgccgagttg atcgacgaga tactgcaccg caccctcaat
      901 gtagcgattg gcttgcgcgg tcggcagccc ggcggtagga cgcagcaggc gcatgccggc
      961 ccacgatgcc agctgttcat gccagcttgc cagacgcacg cgattgttcg tcggatctga
     1021 cttggtaccg gtgagaccaa cggcttcaat gaacacacac ggagggccgt cgatcaccgt
     1081 taccgcatga tggtatttgc caccggtcag aatagcgatc atgtcattct tctggccctc
     1141 ttccgtgcga tggccggtga gaatgatatc gccgcgcacg gtcgaagcgc agaacttgac
     1201 catggcagca tcgccgcggt atcgccgggc ccattccagc atgtgaaccg ggtctccgaa
     1261 acgaggcgag tagaccgcat tcgggtagcg atacaggctg cgcgctttcg ctttcgcccg
     1321 ttcgaggctg ccgaagggtt tttccatggc ctgtttcagc atgggataat acctgggcag
     1381 cagtttcatc gtgatcaatt ctcgttcttc gtcagtcagt atcacgtcgt cagtcggagt
     1441 gcccgaagct gtcacgctgc tggagccact caccgacgga gccgtcacgg agtgatcgtt
     1501 gtaaggggtc gttgagggct gcccaaagac cgcggtgctc aggaacagcc ccaggatcag
     1561 tatggcaatc gccgaagtgg gacggaaagg agggtttcgt tgcatgactg gtctccgttt
     1621 cgatgatttc atcttcatca taccactctt tctccgctca ggttttc
//
LOCUS       DAORHD010001218         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_97161_length_1666_cov_3.612042, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001218 DAORHD010000000
VERSION     DAORHD010001218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97161_length_1666_cov_3.612042"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="PKO06_20265"
     CDS             complement(<1..894)
                     /locus_tag="PKO06_20265"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72052.1"
                     /translation="MTLRMAMEKKRLGDILLGCNLISQEQLGQALAYQKSKGIKLGQA
                     LIEQGIVTEDDIIWALGNQLNISFIHLNPQIINREALKLISAEYARDHKLIPLYQTGT
                     QLSICMVDPLDFDAVETLAAKTQCTVNVSICTSFDFEQTFSAVYGPLEVQENKVTEDI
                     AEKPALERGIPKGMEGPEKVINYLLGQAIINRVDRVHFEPSDKGVVIRFRTNSILSKK
                     LEIPFKMHQEVINKLKTLSQIALGTGASTKTAVGHFRVTISGRQVNVQSLFYPTVNGE
                     MVILKLTDFGENAAEVVQNSKE"
     gene            complement(989..1273)
                     /locus_tag="PKO06_20270"
     CDS             complement(989..1273)
                     /locus_tag="PKO06_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV72053.1"
                     /translation="MKKIELVKEIARNTNLSQNQAAQALEATIDIIKTALRKKEEIRL
                     VGFGSFKVTTRQSRMGVNPQTKKAIKIPATNVAKFVPGKELKDMVNHNRK"
     gene            complement(1378..>1666)
                     /locus_tag="PKO06_20275"
     CDS             complement(1378..>1666)
                     /locus_tag="PKO06_20275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72054.1"
                     /translation="LHSEIEQVRQKLAQAASIARTQATTSRLTTTQPGTLLPTTPTAP
                     TSPTGTTPTSSGTTTTVLQERLRALEERMRVLRDQQQKLLIRLNDLRNRLH"
BASE COUNT          327 a          415 c          502 g          422 t
ORIGIN      
        1 ctctttgctg ttctgaacaa cctcggctgc attctcgccg aaatcggtga gcttgagaat
       61 gaccatttcg ccattcacgg tcgggtagaa cagcgactgt acgtttacct gtcgacctga
      121 gatcgtcacg cggaaatgac cgacggcggt cttggtcgaa gcaccggtgc cgagagcgat
      181 ctgcgacaag gtcttcagct tgttgatgac ttcctggtgc atcttgaagg ggatctcaag
      241 cttctttgac agaatcgagt tcgtacggaa gcgaatgacg acccctttgt cggatggttc
      301 gaaatgcacc cgatcaacgc gattgatgat ggcctggccg agcagataat tgatgacttt
      361 ctcggggcct tccatgcctt tgggaatgcc acgctcgagg gccggtttct cggcgatgtc
      421 ttcggtgacc ttgttttcct ggacttccag ggggccgtaa acggcggaaa acgtctgctc
      481 gaaatcaaaa ctggtacaaa tagaaacgtt gaccgtacat tgggttttgg cggccagcgt
      541 ttcgacggcg tcgaaatcga gcgggtcgac catgcagatg ctcagctggg tgccggtttg
      601 gtacagagga atcagcttgt ggtcacgggc atactcggcc gagatgagtt tcagggcctc
      661 tcgattgatg atctgagggt tcaggtgaat gaacgaaatg ttcaactggt tcccgagagc
      721 ccagatgatg tcgtcttcgg tgacgatgcc ttgttcgatc agcgcctgac cgagtttgat
      781 ccccttgctt ttctggtacg ccagcgcttg acccaactgt tcctgagaaa tgaggttaca
      841 gccgaggaga atgtctccga gtctcttttt ttccatagcc atacggaggg tcatggtacc
      901 tcatctcaag aacgggggca agcggaaccg cacaaaaaca aaaccgccgg aggcatctgc
      961 cccccggcgg tttgtgaatc gctcttgatt acttgcggtt atggttaacc atgtccttca
     1021 gttctttgcc aggcacgaac ttggcgacgt tggtggcggg aatcttgatc gctttcttcg
     1081 tctggggatt gacacccatg cggctctggc gggtggtcac cttgaaggag ccaaaaccga
     1141 cgaggcggat ttcttccttc ttgcgcaaag cggtcttgat gatgtcgatg gtggcttcga
     1201 gagcctgagc ggcctggttc tgggacaggt tggtgttgcg ggcgatttcc ttcacgagtt
     1261 cgatcttttt cacgggatcc tcctgttgga gatagagttt ttaacggact tgacgcatcg
     1321 taccaaaggg gagcgcagcc tgtcaagccc atccggaaaa tatttcaaaa atagttctca
     1381 atggaggcgg tttcgcagat cattcaggcg tatcagcagc ttctgttgct gatcacgcag
     1441 cacccgcatc cgttcttcca gtgcccgcag gcgttcttgc aacacggtcg tggtcgtgcc
     1501 cgatgaagtc ggtgttgtcc cggtcggcga ggtcggggcc gtgggagtgg tcggcagcag
     1561 tgtgccgggc tgggtcgtgg tcagacgact ggttgtggcc tgcgtgcgtg cgatcgaggc
     1621 ggcttgcgcc aatttttggc gaacctgttc gatctcggag tgcaga
//
LOCUS       DAORHD010001219         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_97458_length_1663_cov_3.822761, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001219 DAORHD010000000
VERSION     DAORHD010001219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97458_length_1663_cov_3.822761"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1653)
                     /locus_tag="PKO06_20280"
     CDS             complement(<1..1653)
                     /locus_tag="PKO06_20280"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNV72055.1"
                     /translation="MAGKPLPRIKVDYTNIDKVHFRLVKADWKQLLNEERWSTPEDVR
                     WDKWERLMSYSAAKEWAVTLPPTPDYLARTEFTPFPEMKKGFYHLIASWKDSFAKDNN
                     TLFLTTVFVSDLAMITRNRWGNCETLVVHNESGTVAHKAEVRVYRQDNEGHWRDRGTV
                     KPDANGWCSVKGENGFLLTASQDDDFIAMPNSLSTWQPSEESMQTQVFFFTDRAIYRP
                     GQMIQFKGLLVRIDRQQNQYEVRPNQNVNVVFFDVNRQQIVRQTFTTNEAGSFSGTFT
                     APTDRLPGQMQIVAEAFGGSAVIRVEEYKRPKFKVTLDIPSEGGKLGEKLTVKGNAMA
                     FTGAAIDGAKVKYRVVREVVMPWWCWWRVVRTPSQEIAHGVVTTDIQGKFEIPFVPAP
                     NRTIPESDQPVFTFRISADVTDSTGETRSGQQAIRLGYTALELSLSAGQWLEADQEFK
                     LGVQSQTLDGKGVPANGTLQVFSLKQPVKTVRKAFYDAEGADASDYRRWEQDKVVHES
                     PFNTSVDGTFALPVKLPVGAYRAVASARDRFGKTVKTQFDLLI"
BASE COUNT          334 a          485 c          468 g          376 t
ORIGIN      
        1 gatcagcagg tcgaactgag ttttgaccgt cttgccgaac cgatcgcgcg ccgaagcgac
       61 cgcgcgatac gctccgaccg gcagttttac cggcagggcg aaggtgccgt cgaccgacgt
      121 gttgaacgga gattcatgaa cgaccttgtc ctgctcccag cggcgatagt ctgaagcatc
      181 agcgccctcg gcatcataaa acgcctttct caccgtcttt accggctgct tgagcgaaaa
      241 cacctgcagc gtgccattgg ccggcacccc tttgccatcg agggtctgtg attgtactcc
      301 gagtttgaac tcctggtctg cctcgagcca ctgtcccgcg ctcagactca gctcaagagc
      361 ggtataaccg aggcgaatgg cctgctgacc actgcgggtt tccccggtag aatcggtgac
      421 atcggcggag atgcggaaag tgaagacggg ctgatcggat tccggaatcg tccgatttgg
      481 cgctggaaca aacggaattt cgaacttgcc ctggatatcg gtggtcacga ctccgtgggc
      541 gatttcctgc gacggcgtgc gtaccacgcg ccaccagcac caccacggca tgaccacctc
      601 gcgaacgacg cgatacttta ccttggcgcc atcgatggcc gccccggtga aagccatggc
      661 gtttcccttg acggtgagtt tctcaccgag tttgccacct tcagagggaa tatcgagcgt
      721 gactttgaac ttcggtcgct tgtactcttc aacccgaatg acggccgagc caccgaatgc
      781 ctcggcgacg atctgcatct ggcccggaag tcgatcggtc ggcgcggtaa aagttccgct
      841 gaatgaccct gcttcgttcg tcgtgaaagt ctggcgcacg atctgctgtc gattcacatc
      901 gaagaagacc acattgacat tctgattcgg gcgcacttca tactggttct gctgtcgatc
      961 gatacgcacc agcagaccct tgaactgaat catctgacca ggccgataga tcgcgcggtc
     1021 cgtgaagaag aagacctgtg tttgcatcga ttcttcagag ggctgccagg tgctgagcga
     1081 gttgggcatg gcgatgaaat catcatcctg agaggcggtc agaagaaagc cgttctcgcc
     1141 cttcaccgaa caccagccgt tggcatctgg tttgaccgtg ccgcgatcgc gccagtgacc
     1201 ttcgttgtcc tgacggtaca ctcggacttc tgccttgtgt gccaccgtac ctgattcgtt
     1261 gtgaacgacc agcgtttcac agttgcccca ccggttgcgc gtgatcatcg ccagatcaga
     1321 gacaaagacc gtggtcagaa acaacgtgtt gttgtctttc gcaaacgagt ctttccacga
     1381 ggcaatcaga tgatagaacc cctttttcat ctcggggaac ggcgtgaatt ctgtgcgggc
     1441 gagataatcc ggggttggtg gcaaagtgac ggcccattct ttggccgcgg aatacgacat
     1501 gagtcgttcc catttgtccc agcgaacatc ttccggggtg ctccagcgct cctcgttcaa
     1561 cagctgtttc cagtctgcct tcaccagccg gaagtgcacc ttgtcgatgt tggtgtagtc
     1621 gaccttgatg cggggaagtg gtttgccggc cacgcgctcc gtc
//
LOCUS       DAORHD010001220         1658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_97953_length_1658_cov_3.127261, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001220 DAORHD010000000
VERSION     DAORHD010001220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97953_length_1658_cov_3.127261"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1658)
                     /locus_tag="PKO06_20285"
     CDS             complement(<1..>1658)
                     /locus_tag="PKO06_20285"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV72056.1"
                     /translation="RIRVIESPSHPIRSEIEDTRAVVSFSQGSAVMDRDFVLSLELAE
                     TMPLIASQANFNNRDHLLLEFRTDLPEDEQPLPRLVDFVIDCSGSMGGESIAEARRAV
                     ELCIRSLNEGDLFQIVCFGSSFRFVFDSYLPFNQQTLEQAVKAIAAIDADMGGTELLA
                     PLQAVLGEKSPGLTGLLRSTAAALLGKTTSRPATLENRRRTMIIMTDGEVSNDDAILD
                     YVRLNAGNTRIFSFGIGAGASHYLVKGLARQSRGAAEFIFPGERIEPKVLRQFGRIDT
                     PLIEEVAIDWGGLSVEMAPQPLPTIFSGEPVLIAARLSTSEAGKGGLSLVLSGKVNGK
                     PHSWPVTTRPAAGSRNVALTWARLAIRDLEEGTGTAKGSNQKRGQTNRALKIAKEYGL
                     TCRDASWVAIEQRSAADKTQSPAELRRVPVMVTSGWHDTSGGKRGVVGGGILACLTNT
                     VCAQPMSVYAAMPTGPKKSRARVVSEAADVDDGFACAPLNRAADVLSTKSDPKLWYLD
                     LLQQQKADGSFTCTPLLESLSNVPLTELKAWASGLDCDDAARLD"
BASE COUNT          334 a          508 c          495 g          321 t
ORIGIN      
        1 catcgagacg cgccgcatca tcgcaatcga gaccgctcgc ccaggctttc aattcagtca
       61 agggcacgtt ggaaaggctc tcgagcagtg gcgtacacgt aaacgatcca tccgccttct
      121 gttgctgaag caggtcgaga taccacagct tcggatcgct cttcgtcgag agcacatctg
      181 ccgcgcgatt caacggggcg caggcaaaac catcatcgac atctgcggcc tcggacacca
      241 cgcgcgcgcg gctctttttc gggccagtcg gcattgcggc gtacacgctc atcggctgag
      301 cgcagacagt gttcgtcagg caggccagta tgccaccgcc gacgactccc ctcttgccac
      361 ccgacgtgtc gtgccagccg ctcgtcacca tgaccggaac acgccgcagt tcggcggggg
      421 actgggtctt gtcagcggcc gaccgttgtt cgatcgccac ccacgaagca tcgcggcatg
      481 tcaggccata ctccttcgcg attttcagcg cccgatttgt ctggccacgt ttctggttcg
      541 agcctttcgc ggtgccggtt ccctcttcga ggtcacgaat cgccaggcgg gcccaggtga
      601 gcgctacgtt gcgcgacccc gcggcaggcc gcgtcgtcac cggccaggaa tgtggtttcc
      661 cgttcacttt tcccgaaagt actagtgaaa gacctccttt gccggcttcg gatgtgctga
      721 gacgggcggc aatcaataca ggttctcccg agaagatcgt cggcaggggc tggggagcca
      781 tttcgaccga caggccgccc caatcgatgg cgacttcctc gatcaatggt gtgtcgatac
      841 gcccgaactg ccgcagcact ttgggctcga tgcgctcgcc gggaaaaatg aactcagcgg
      901 ctccgcgact ctgacgagcc agacctttga ccagataatg acttgccccc gcaccgatgc
      961 cgaaggaaaa gatgcgggtg ttgcctgcat tgagccgcac gtagtcgaga atcgcatcgt
     1021 cgttcgacac ttcgccatcg gtcatgatga tcatggttcg acgccggttt tcgagcgttg
     1081 caggtcggct cgttgtcttg ccgagcaggg cggcggccgt cgagcgcagc agcccggtca
     1141 ggcctggaga cttttcgccg agaacggcct gcaatggagc gagcagctca gtgccgccca
     1201 tgtcggcatc gattgcagcg atggccttca cagcctgttc aagggtctgc tggttgaagg
     1261 gcagatacga gtcgaagacg aaccggaatg atgacccgaa acagacgatc tgaaacagat
     1321 caccttcgtt caacgagcga atacacagtt cgacggcccg acgcgcctcg gcgatcgact
     1381 ctccgcccat ggatccggaa caatcaatga cgaagtcgac cagacgcggc aggggctgct
     1441 cgtcttcggg aagatcggtt cggaactcaa gcaggaggtg gtctcggttg ttgaagtttg
     1501 cctgcgaggc gatgagcggc atggtttcag ccagctcgag cgacagcaca aagtcgcgat
     1561 ccatgacggc ggaaccctgg ctgaacgaca caaccgcacg cgtatcttca atctcggagc
     1621 gaatcggatg tgaaggagac tcgatcaccc gaattctg
//
LOCUS       DAORHD010001221         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_98586_length_1651_cov_3.413534, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001221 DAORHD010000000
VERSION     DAORHD010001221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98586_length_1651_cov_3.413534"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKO06_20290"
     CDS             complement(<1..825)
                     /locus_tag="PKO06_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72057.1"
                     /translation="MTTTINRNQILRLTRETIDNTLRFFHTVNENLFTLVNRLPHAEV
                     RPMNLDEITRICNDFQRNHETRVTQLTTIWNELHTHLRNQVPTTPINLFDTNEIIRLN
                     REMIDHTMRFFTTTNEALLHGRLPETPEVLPRSVIETITNAVQNFQRTQNEVIHTFEQ
                     TFHKVINTIHPTTTERPTETQVHNTPINVVRDEIFTLNRDIIENTIRFFMTLNEHVVA
                     IVENHDQKHVTTHTEQINHIHQNFLNTHRTIIDRLNTCIRRTAESLVRLLLAPARRL"
     gene            1015..>1651
                     /locus_tag="PKO06_20295"
     CDS             1015..>1651
                     /locus_tag="PKO06_20295"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV72058.1"
                     /translation="MVQFAGFFSWKLFGGLFLGWGIGANDSANIFGTAVATNSVRYKT
                     AVWLIAIFVVLGASCQGWKLYKNYEFGKKKQSQSETGSTASPTTATVSDSAFTDALLT
                     TLAAAIVVLLATVAAIPASTSQAAIGACLGLAIAAGGGIDAVDWGAFGKMLFCWVLNP
                     FGAALVTVVLVKVFTAVLRRRYGKNLSQLNQVYTILLLVAGCYGAYELGANN"
BASE COUNT          307 a          408 c          526 g          410 t
ORIGIN      
        1 cagccgtcgc gccggcgcca gcagcagccg caccagcgac tcggcggtgc ggcgaatgca
       61 ggtgttcagc cgatcgatga tcgtgcgatg cgtgttcagg aagttctgat gaatgtggtt
      121 gatctgttcg gtgtgcgtcg tgacgtgctt ctgatcatgg ttttcgacga tcgccaccac
      181 atgttcgttc agggtcatga agaagcggat ggtgttctcg atgatgtcgc gattgagcgt
      241 gaagatctcg tcgcgcacca cgttgatcgg ggtgttatgc acctgagttt cggtggggcg
      301 ttcggtggtc gtcggatgga tggtgttgat caccttgtgg aacgtctgtt cgaaggtgtg
      361 aatgacctcg ttctgcgtgc gctggaagtt ctggaccgcg ttggtgatgg tttcgatcac
      421 gctgcggggc aacacttcgg gcgtctcggg caggcgacca tgcagcaggg cttcgttggt
      481 cgtggtgaag aaacgcatgg tgtgatcgat catttcacga ttgaggcgaa tgatctcgtt
      541 ggtgtcgaac aggttgatcg gggtggtcgg aacctggttg cgcagatggg tgtgcagttc
      601 gttccagatg gtggtgagct gggtcacgcg cgtctcgtga ttgcgctgga agtcattgca
      661 aatgcgggta atctcgtcga gattcatcgg gcgaacttcg gcgtgcggca ggcggttgac
      721 cagcgtaaaa aggttctcat tgaccgtatg gaaaaagcgc agcgtgttgt caatggtttc
      781 gcgggtaagc cgaaggatct ggttccgatt gatcgtcgtg gtcatggtct cgagactccc
      841 ttatgatggt tattcggtcg gctgaaacgc cgttccggtt attcactgac ttctgcggcg
      901 gccctgaaag cggtcgattg aaccaccggt catgaaagta tacgaatcaa acgcccaacc
      961 cggaagaccc tggtgaaaaa aacatgatat gatcgcttca gggagtattc gagtatggtt
     1021 caattcgccg gtttcttttc ctggaaactg tttggtggtc tgtttcttgg ctggggcatc
     1081 ggggccaacg attcggccaa tatcttcggc actgccgtcg ccaccaactc ggtgcggtac
     1141 aagacggccg tctggctcat tgccattttt gtcgtattgg gcgcatcctg tcagggctgg
     1201 aagctgtaca aaaactacga gttcggcaag aagaagcaga gccaatcaga gactggcagc
     1261 accgcaagtc cgaccacggc aactgtgtct gattcggctt tcaccgatgc tctgctgacc
     1321 acactcgcgg cggccatcgt ggtcttgctg gccaccgtcg cggcgattcc ggcgtctacg
     1381 tctcaggccg cgatcggtgc ctgtctgggc ctggctatcg cggcaggagg aggaatcgat
     1441 gccgtcgact ggggagcctt cggtaaaatg ctgttctgct gggtcctcaa tcccttcgga
     1501 gccgctctgg tcaccgtagt cctggtcaag gtcttcaccg ccgttctccg acgccggtac
     1561 ggcaagaatc tgtcacagct gaatcaggtc tataccattc tgctgctggt cgccggatgc
     1621 tatggagcct atgaactggg tgcgaacaat g
//
LOCUS       DAORHD010001222         1651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_98622_length_1651_cov_2.503759, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001222 DAORHD010000000
VERSION     DAORHD010001222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98622_length_1651_cov_2.503759"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PKO06_20300"
     CDS             <1..699
                     /locus_tag="PKO06_20300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72059.1"
                     /translation="REPYNPGGMEWGSEPTTSKVTFWSYVHDLYGLKSVDLQYAFVTN
                     GGAAIGADANWKSLPLQAREIPAQTNPKPLIKAAEYSATLDGAAGKTVAYRVRALDNK
                     GNASVSPTQYVFIGTGAPTQPDKPCWSPAEPTMADTIVIRSDRPGFVHWGINGWRQPP
                     EALWPAGTKPWPDGKSVETPLVSVPGTDGYEARLGGFSQGTIPLSSIEFVFHHTDGSW
                     GKDHRITVKSSGEK"
     gene            696..1469
                     /locus_tag="PKO06_20305"
     CDS             696..1469
                     /locus_tag="PKO06_20305"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV72060.1"
                     /translation="MMDSFFNSLRFPVMIILTIFVTINFFEERESQRRQQMNAVKQQG
                     KSLPAEATIEVSKYDLYKTYPANRNALHQAALTGNVEVAEMSIQQGIPLFSADANGCT
                     PLHLAATYGHVDVVKLLIKHKAPLDVTESLGRTPLYVAAHEKQFKVVDILIEAGADIK
                     GKDTFGISLIHVAAQSGNVKLAEHLLLNGVPVLETNFRGWTPLHYAAINGHTEIAKFL
                     LARGGYNRQRIDYGWTAEDLARGHNHVEIVKLLENRAEQ"
BASE COUNT          396 a          469 c          460 g          326 t
ORIGIN      
        1 cgcgaaccgt acaaccccgg gggcatggaa tggggctctg aaccaacgac gtcaaaggtt
       61 acgttctgga gctacgtgca tgatctgtat ggcctgaaat cagtcgacct gcagtacgcg
      121 ttcgtaacga acggtggagc cgccatcggc gcagatgcca actggaagtc cctgccgctg
      181 caggcgcgcg aaattccggc gcagaccaac cccaaaccct tgatcaaagc cgctgagtat
      241 tccgccacac tcgatggggc ggccggaaag acggtggcct accgggtgcg agctctcgat
      301 aacaagggga acgcctcggt cagtccgaca cagtatgtct tcatcggaac cggagcaccg
      361 acccaaccgg acaaaccctg ctggagcccg gccgagccga ccatggctga taccatcgtc
      421 attcggtccg atcgccccgg gttcgttcac tggggaatca acggctggag gcagcccccg
      481 gaagccttgt ggccggctgg taccaagccc tggccggacg gcaaatcggt cgagactcct
      541 ttggtttctg tcccaggaac ggatggctat gaggcccgac tgggagggtt ttcacagggc
      601 acgatcccgt tatcgagtat cgagtttgtt tttcaccaca ccgatggttc gtgggggaag
      661 gatcatcgta tcaccgtcaa gagttcggga gaaaaatgat ggactcgttc ttcaacagcc
      721 ttcgctttcc cgtcatgatc attctgacaa tcttcgtgac gatcaacttt ttcgaagagc
      781 gtgaaagtca gcggcgacag cagatgaacg ccgtcaagca gcaggggaag tccctgccgg
      841 ccgaagcgac cattgaagta tcgaagtatg atctgtacaa aacctatccc gctaaccgaa
      901 acgccttgca tcaggccgct cttaccggga atgtcgaagt tgccgagatg tcgattcaac
      961 agggaatccc cctgttcagc gccgatgcga acggatgcac gccgctgcat ctggccgcga
     1021 cctacgggca cgttgatgtc gtcaaactgt tgatcaagca caaggctccc cttgatgtga
     1081 ccgaatcact gggtcggacg cccttgtatg ttgcggcaca cgaaaagcag ttcaaggtgg
     1141 tcgacattct gatcgaagcg ggcgcggaca tcaaaggcaa ggataccttc ggtatttccc
     1201 tgattcatgt ggctgcccag agcggaaacg tcaaactcgc tgaacacctt cttctgaatg
     1261 gcgttcccgt tctcgaaacg aatttccggg gttggacgcc gctgcattat gcggccatca
     1321 acggacacac cgaaatcgcc aagtttctgc tcgcacgcgg cgggtacaat cgtcaacgta
     1381 tcgactacgg ctggaccgcc gaagacctgg ctcgcggtca caaccacgtc gaaatcgtca
     1441 agttgttaga gaaccgtgcc gagcagtaac attctctgac caataaacga gaaacaaacc
     1501 aaaaacaaag gaaagataac gacatgaatc aagcagatgt cctgtttacc aatgcctggg
     1561 tcctcaccat ggatgaggat ttccggcagt attgtcccgg tgcggtcgcc gtgtcaggcg
     1621 acaagatcgt cgccgtgggt gcaaaaagcc a
//
LOCUS       DAORHD010001223         1650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_98732_length_1650_cov_2.495298, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001223 DAORHD010000000
VERSION     DAORHD010001223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98732_length_1650_cov_2.495298"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="PKO06_20310"
     CDS             <1..1344
                     /locus_tag="PKO06_20310"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNV72061.1"
                     /translation="ARVWLITPQVASETPMRQIKVELTEDHDKLAWVRVYHLVGGKRA
                     GQKAETTLMEKGESQLVTVAPNDLLCVVALNIGMREPRRASVRVVDASIELSVDPPRF
                     DLTDQHQVLQLTAMANNLPPNVKRAGFEVQFGNQPPQALKVHDDTSGTLFCQLTDKFG
                     CFGDTTIAIRLYDRTTGLKKPLLAEVKVPVIVKMDPPILMLREDVKADVGKPTAIVAS
                     AQNISAGSTYEWDYGDGTPPEKTDGAQTTHVFAKDGKFRVHVRLLDSGGKELTTSWCF
                     ATALVGKTIEIRKTYHPNGQLKSEEPIYWGYRVPSMPETKYAVIHGLSREWDDKGILI
                     YESTYVDGKRTGKYKSYHRHNGEIREEGQYENSQPCGLWKEYDDIGQVMAEGRYEKGQ
                     KQGVWRERYYHASHVRDQRLVKIYTGSDNTYLDGRRIDSKGWGKYEEKVGPIYKP"
     gene            1421..>1650
                     /locus_tag="PKO06_20315"
     CDS             1421..>1650
                     /locus_tag="PKO06_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72062.1"
                     /translation="MRWKDFLLGLVFVLFPIALAMLGNSLLFDDPVRIVQEQLEERLA
                     VAMDEVESRKDYAEQFRRVFRAVENRVFRAADP"
BASE COUNT          400 a          437 c          503 g          310 t
ORIGIN      
        1 gcccgcgtgt ggctcatcac accgcaggtc gcgagcgaga caccgatgcg acagattaag
       61 gtcgaactca ccgaagatca cgacaaactc gcctgggtgc gagtctacca tctcgtcggc
      121 ggcaaacgcg ccgggcagaa ggccgagacg acgctcatgg aaaaaggcga gtcacagctg
      181 gtcaccgtcg cgccgaacga tctgctctgc gtggtcgcgt tgaacatcgg tatgcgcgaa
      241 ccgcgtcggg cttcggtacg agtcgtcgat gcttcgattg agctttccgt cgacccgccg
      301 cgattcgatc ttaccgatca gcatcaggtg cttcagctta ccgccatggc gaacaatctg
      361 ccgccgaacg tgaagcgggc cgggttcgaa gtgcagttcg gcaaccagcc gccacaggct
      421 ctcaaagtcc atgacgatac ctccggcacg ctgttctgcc agctcaccga caagttcggc
      481 tgtttcggcg acacgaccat tgcgattcgc ctctacgatc gcacgacggg cttgaagaag
      541 ccgctgttgg ccgaagtaaa agtaccggtc atcgtcaaga tggacccgcc catattgatg
      601 ttgagagaag atgtgaaagc ggatgtcggc aagccgaccg cgatcgtcgc ttcggcgcag
      661 aacatttccg cgggcagtac ctacgagtgg gattacggcg acggcacacc ccccgagaag
      721 acggatggcg cgcagacaac acatgtgttt gcgaaagacg gaaagttccg cgtccatgtc
      781 cgtctgctcg acagcggcgg caaggagctc acgacaagct ggtgtttcgc gacggcgctg
      841 gtgggcaaaa ccatcgagat tcgcaagacc tatcacccga acggccaact gaaatctgaa
      901 gaaccgatct actggggtta tcgcgttccc agcatgcccg agacgaagta cgccgtcata
      961 cacggattga gcagggagtg ggatgacaag gggatcttga tctatgaaag tacgtatgtc
     1021 gatggcaagc gcacgggtaa gtacaagtcg tatcatcggc acaatggcga aatacgagaa
     1081 gaaggacagt atgagaacag tcagccgtgc gggctatgga aagagtatga cgatatcggc
     1141 caggtgatgg ccgaaggcag gtatgagaaa gggcagaagc agggcgtgtg gcgtgaacgc
     1201 tattatcatg cctcgcatgt gcgcgaccaa cgcttggtca agatatacac cggcagtgac
     1261 aacacctatc tcgatggaag gcgcatcgac tcgaagggct ggggaaagta cgaagaaaaa
     1321 gtcggcccga tctacaaacc ctgaagaact ctacagagag tgcgagagtc ctgtatcatg
     1381 aggtcggtct gaaagatcag gcgtcaggat acgttgaccg atgaggtgga aagactttct
     1441 tctgggtctc gtcttcgtgc tgttcccgat cgcgttggcg atgctgggaa acagcctgct
     1501 gtttgacgac cccgtcagaa tcgttcagga acagcttgaa gagcgtctgg ctgtcgccat
     1561 ggacgaggtg gaatccagaa aagactatgc agagcagttc cgccgggtgt ttcgtgccgt
     1621 cgaaaaccgg gtgtttcgtg ccgctgatcc
//
LOCUS       DAORHD010001224         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_98805_length_1649_cov_3.224592, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001224 DAORHD010000000
VERSION     DAORHD010001224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98805_length_1649_cov_3.224592"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..770
                     /locus_tag="PKO06_20320"
     CDS             156..770
                     /locus_tag="PKO06_20320"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV72063.1"
                     /translation="MTDSLIGFLQAGGIMMVPLTLIGILIFASGTYQLAWLAMWFWSP
                     ERYSCSGAPFWAARAHALARTRTGWQGLSLLESIELCLAKTEHCLTRRVPTMRFLAQI
                     STLFGFLGTVTGMVQVFQTVAARGVVTPKDLAGGIYEALFTTVYGLVLALIAYALAHF
                     IESLARRHLSLLEMQILKELDSDGANGVSSSPPSAVVSVPGAEV"
     gene            767..1222
                     /locus_tag="PKO06_20325"
     CDS             767..1222
                     /locus_tag="PKO06_20325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV72064.1"
                     /translation="MSLRRRAQKSEPFLDMTPCSDIMFTLLLFYILTQSFVTQMPVQL
                     PRLATGESVVDARQIRLQIERDGQVVCQSAFSSVPPSTARRSASECRLEPPWERSLPR
                     VLPASDPAVLVVVHREAPAGIAVELLDALRRLGVPSISFAGLPRTESIE"
     gene            1219..>1649
                     /locus_tag="PKO06_20330"
     CDS             1219..>1649
                     /locus_tag="PKO06_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72065.1"
                     /translation="MNFRTNERPITLLTLVVCVGAGVVLQAGVVHALAAWCSSCKTLP
                     ENLAIVRLQLATIRPPELVLPPKIVEPAPIPPIPRPENPPSPAHEEPSQVIEPAKPPD
                     VVSPPMAPKPPEELKPVLRPPTRRPRPEKKPVVVPREAQVPT"
BASE COUNT          289 a          501 c          514 g          345 t
ORIGIN      
        1 tgcgacccat gagccggagg tgttttccgg gtggcctgcg cagacgctca gtctgtaccc
       61 gcggcgagac caggatcagg catcgatttc ggcatcgccc gccgaggcgg gcaagagcgt
      121 gatcgccgcg tgaacgcctt tgaggggagt tgagcatgac tgattctctg atcggtttcc
      181 tgcaggccgg cggcatcatg atggtgccgc tgacgcttat cggcatcctg atctttgcca
      241 gtggtacgta ccagctggca tggttggcga tgtggttctg gagcccggaa cgatattcgt
      301 gcagcggtgc tccgttctgg gcggcccggg cgcacgcctt ggctcgaact cgaaccggat
      361 ggcagggact gtcgctgctc gaatcaatcg aactgtgtct ggcgaagacg gaacactgtc
      421 tcacccgacg cgtaccgacg atgcgattcc tggcgcagat cagcacgctg ttcggcttcc
      481 tcggcacggt gaccggcatg gtgcaggtat ttcagaccgt cgccgcgcgt ggcgtcgtca
      541 ccccaaagga tctggccggc ggcatctatg aagctttgtt taccacggtg tacggtctgg
      601 tgcttgccct catcgcgtat gcgctggcgc atttcattga gtcactggca cgacggcatc
      661 tgagccttct tgaaatgcag atattgaagg aactcgactc ggacggggcg aatggcgtgt
      721 cgtcgtcgcc accatcagca gtggtatcag taccgggagc agaagtatga gtctcagacg
      781 tcgagcgcag aagagcgagc cgtttctgga catgacgccc tgttctgaca tcatgttcac
      841 gctgttgctc ttctacattc tcacgcagag ctttgtgacg cagatgcccg tgcagttgcc
      901 gcgactggcg acgggtgagt cggtggtgga tgcccggcag attcggctgc agatcgaacg
      961 tgatggtcag gtcgtttgtc agtcggcgtt ttccagcgtt ccaccgtcga cagcacggcg
     1021 aagcgccagc gaatgccggc tcgaaccgcc gtgggaacgc agtctgccgc gggtgctgcc
     1081 cgcgtctgat cccgcggtgc tggtggtcgt gcatcgtgaa gcccccgcgg gtatcgccgt
     1141 ggaactgctc gatgcgctgc ggcgtctcgg cgtgccgtcc atttccttcg cgggcctgcc
     1201 acgcacggag agcattgaat gaacttccgg acgaatgaac gaccaatcac gctcctcacc
     1261 ctggtggtgt gtgtcggtgc gggggtcgtt ctgcaggccg gtgtggttca cgcccttgcc
     1321 gcctggtgtt cttcgtgcaa gactcttcct gagaaccttg cgattgtccg acttcagttg
     1381 gcgacgatac ggcctccgga gctagtattg ccaccaaaga tcgtcgaacc cgcgccgata
     1441 ccacccatac cacgaccgga gaatccaccg tcgcccgcac acgaagaacc atctcaggtc
     1501 atcgagcctg cgaaaccgcc agatgtcgtt tctcctccga tggcaccgaa accacctgag
     1561 gaactgaaac cggttctgcg tcccccgacc cgcaggcccc gtccggagaa aaagccggtc
     1621 gtcgtccccc gcgaggccca ggttccgac
//
LOCUS       DAORHD010001225         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99043_length_1647_cov_2.836055, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001225 DAORHD010000000
VERSION     DAORHD010001225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99043_length_1647_cov_2.836055"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1647)
                     /locus_tag="PKO06_20335"
     CDS             complement(<1..>1647)
                     /locus_tag="PKO06_20335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72066.1"
                     /translation="GLNITTDFEKRGNIVVEVTVSYTNYIKKCTIKKPFFVVRLLPAP
                     FWKFTLFASRGASVAQSDVNRLAEVADDGKTTGAPPLILFNRLLNDKSANQADLDFFN
                     VFVDPNTILPNSQALEESGWVYLGGFGKSNDNWKDSKNTGDPNSLNNLTLNVLSGTNG
                     PFDQGYYGEGFHYFFTNESFGWLCADDYSAAINQLNGADKSVSVAYNDFGFFKGQRTA
                     KFGPSGQEQDLFKPLYDALKTKFPQEVFDHGSALHLFGTPNLCTPTLVFGKVKRRFLR
                     TYGFLFNAPGFNRVYPLRVVDKQAWDNGVIAAEVAAWLTSKGLSAAATTAIDNAMANI
                     WPTPIGQGPLDGAAYTTGFPPNFPPLQPGLKDDEPYMMGLKNIKSPRGPEKPWQTVFS
                     GFKYIDKGPEALLDSSYSFTADPELDYSGSISSIKPPQNYLRDKVSYEISDSSLPKPM
                     QLSQIPFLTTGSDAILDITQTPHIMSLHQVIAVDGDLVIDKPIVVAQGGIILVNGEIT
                     IKAPIINPFIHNFPNPPTSPHMFGYLTLVSEKGIRVELEA"
BASE COUNT          360 a          436 c          468 g          383 t
ORIGIN      
        1 gcgcttcgag ttcgacgcga atgccttttt cggaaaccag tgtcagatac ccaaacatgt
       61 gaggactggt cggcggattc ggaaagttgt gtatgaacgg attgatgatc ggcgctttga
      121 tggtaatctc gccattcacc agaatgattc cgccttgagc gaccacgatc ggtttgtcaa
      181 tcacgagatc gccgtcaacc gcaatgacct ggtgcagtga catgatgtgc ggcgtctgtg
      241 tgatatcgag aatggcgtca ctgcccgtgg tcaggaatgg aatctgcgac agctgcatcg
      301 gcttcggaag cgacgagtca gaaatctcgt aactgacttt atcgcgcaga taattctgtg
      361 gtggcttgat cgagctgatg ctgcccgagt aatcgagttc cgggtcggcg gtaaagctgt
      421 agctggagtc gagcagagct tcaggcccct tgtcgatata tttgaacccg ctgaaaaccg
      481 tctgccaggg cttttctggc ccgcgtgggc tcttgatgtt cttcagcccc atcatgtagg
      541 gctcatcatc tttcaggccc ggctgcaaag gtggaaaatt cggcggaaag cccgtcgtgt
      601 aggccgcacc gtcaagcgga ccctgcccaa tcggagtcgg ccagatgttg gccatcgcgt
      661 tatcgattgc agttgtagcg gcggcgctga gtcccttgga ggttaaccag gcagcgacct
      721 ctgctgcgat cacgccgttg tcccacgcct gtttgtcgac aacacggagc ggatacacgc
      781 gattgaagcc aggtgcatta aacaggaacc cgtacgttcg cagaaaacgg cgtttcacct
      841 tgccgaagac cagagtcgga gtacagagat tcggtgtgcc gaatagatgc agggccgagc
      901 catgatcaaa cacttcctgc ggaaattttg tcttcagggc gtcataaaga ggcttaaaaa
      961 gatcctgttc ctggccagac ggcccgaact tcgcggttcg ctgtcccttg aaaaagccga
     1021 aatcgttata ggcaaccgaa accgatttat cggccccatt caattgattg atcgcggctg
     1081 agtagtcgtc cgcgcacagc cagccaaatg actcattggt gaaaaagtaa tgaaagcctt
     1141 cgccgtagta tccctggtcg aagggtccgt tcgtacccga gagcacgttc agcgtcagat
     1201 tgttgagcga attcggatcc cccgtgttct tcgaatcctt ccagttgtcg ttcgacttgc
     1261 caaaaccgcc gagatagacc catccgctct cttcgagagc ttgcgagttc ggcaggatcg
     1321 tgttcgggtc gacgaagaca ttgaagaaat cgagatccgc ctgattcgcc gacttgtcgt
     1381 tcagcaggcg gttgaagagg atcaagggcg gcgccccggt ggtcttccca tcgtctgcga
     1441 cttcggccag gcggtttaca tcagactgtg ccacactggc tccccgcgag gcgaaaagcg
     1501 tgaacttcca gaatggggcc ggcaacagtc gcaccacgaa gaacggcttc ttgatcgtgc
     1561 atttcttgat gtagttggtg tagctcacgg tgacttcgac cacgatgttg ccgcgcttct
     1621 cgaagtccgt tgtgatgttc aatcccg
//
LOCUS       DAORHD010001226         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99051_length_1647_cov_2.687186, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001226 DAORHD010000000
VERSION     DAORHD010001226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99051_length_1647_cov_2.687186"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKO06_20340"
     CDS             complement(<1..724)
                     /locus_tag="PKO06_20340"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNV72067.1"
                     /translation="MALPLVIDAAFTPGEVQNLSRKVCIVVDVIRSSSTLAVIMSRNP
                     HQVILTPTVQKALKYASQQKVHPVLCGERGGLPPEGFDHGNSPREYLNVDLTGKAVLF
                     TSSNGTRAIADIAMAPHVLMGSFLNSSAVVEEALRLAQGNGLDIIVVCAGREEKFALD
                     DAYCAGHLIARLAGSLGMDQPFVLADGGQAALAIWGYYRDPERLLRQSGSGQEIEKIG
                     LGEDLNFLTQLDTFSCVPSLINR"
     gene            complement(724..849)
                     /locus_tag="PKO06_20345"
     CDS             complement(724..849)
                     /locus_tag="PKO06_20345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72068.1"
                     /translation="MMYGLALIIAIGCFTTLVAEPERWLARLANFLRDFKLHQGF"
     gene            complement(931..>1647)
                     /locus_tag="PKO06_20350"
     CDS             complement(931..>1647)
                     /locus_tag="PKO06_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72069.1"
                     /translation="VQLSFDDVVKRLFLMSFDPYHSVELRWGATGNELSTATDSPMKM
                     KFYRDEKRLRHQLERLYNCQTGLDLGPASPPDVDVRQWLARYLQGQGSTMVLAQLPTA
                     MPVSPMPAPVIPDQSQGMAEGKPALPAAPVLPERRAEPRLVFTSQAATSPVPGSTELP
                     QPVVVESIRVETATVPVIDPVARQKKPAQVATSREYYQFAGRVLNDVDQVLAGMFRAT
                     DGALAKNAGTSAEGDAEFTP"
BASE COUNT          377 a          504 c          438 g          328 t
ORIGIN      
        1 cccgattgat cagactcggc acgcaggaaa aggtgtcgag ttgtgttagg aagttcaggt
       61 cttcacccag accgatcttc tctatctcct gaccggaacc actctgtctc agaaggcgtt
      121 cggggtcacg gtagtagccc cagattgcca aggccgcctg tcccccatcg gccagcacaa
      181 agggctgatc catgcccagg cttccagcca gcctggcgat caggtgtccg gcacaatagg
      241 cgtcgtcaag ggcaaacttc tcttctcgac ccgcgcacac aacgatgata tcaagtccgt
      301 tcccttgcgc cagacgcagc gcttcctcaa ccacggcaga cgaattaaga aagcttccca
      361 ttaaaacatg gggagccata gcgatatccg caatagctcg agttccgtta ctggacgtga
      421 aaagaaccgc cttgcccgtc agatcaacat tcagatactc tcgcgggctg ttcccatgat
      481 cgaatccctc cggcggcaat cctccgcgtt cgccgcacaa cacgggatgc actttctgct
      541 gcgaggcata cttcaaggcc ttctgaaccg tcggggtcag aatcacctga tgtggattcc
      601 gcgacatgat cacggcgagc gttgatgaag agcggatgac atcgaccaca atgcacacct
      661 tccgggagag gttctgtacc tctcccgggg tgaaggcggc atcgatgacc agaggcagag
      721 ccatcaaaat ccctgatgca gtttgaagtc ccgcagaaag ttggcgagac gggccagcca
      781 tcgctcgggt tcagcaacca gagtggtgaa gcagccaatc gctatgatca gtgcgaggcc
      841 atacatcatg ttcatcctcc ttgatactcg ttactcgcta cgtgccttac ctgtaccatc
      901 ggcttttcca cttccaaatc tgacttcatt tcagggagta aattcggcat ctccctcagc
      961 gcttgtgccg gcgtttttcg ccagcgcgcc gtccgtcgcc ctgaacatcc ctgccagcac
     1021 ctggtcaaca tcgttcaaga cccggccagc aaactggtaa tattcacggg aagtagcaac
     1081 ctgagcgggt ttcttctggc gagcaaccgg atcaatcacc ggaaccgttg ccgtctcgac
     1141 acgaattgat tcaacaacca ccggttgcgg caattcggtc gaaccaggaa ctggggacgt
     1201 cgcggcctga ctcgtaaaca ccagacgcgg ctcagcgcgc cgttcaggta acacaggggc
     1261 cgcgggcagc gcgggcttgc cttcagccat cccctgagat tgatccggaa tcacgggagc
     1321 cggcatcgga gacaccggca tagccgttgg cagctgcgcg agaaccatcg tcgaaccctg
     1381 gccctgcaga tacctggcaa gccactggcg aacatcgaca tccggaggac tcgcagggcc
     1441 gagatcgagc ccggtctggc agttatagag tcgctcgagc tggtggcgca agcgcttctc
     1501 gtcgcgatag aacttcattt tcatggggct gtcagtggca gtcgacaact cgttccccgt
     1561 agcaccccat cgaagctcaa cagaatgata ggggtcgaac gacatgagga acaatcgctt
     1621 gaccacgtcg tcaaaactca gctgaac
//
LOCUS       DAORHD010001227         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99137_length_1646_cov_2.949717, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001227 DAORHD010000000
VERSION     DAORHD010001227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99137_length_1646_cov_2.949717"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PKO06_20355"
     CDS             complement(<1..869)
                     /locus_tag="PKO06_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72070.1"
                     /translation="MTAPLVRELRRWLLILLFTGLPLYLIWLVFERQEHDREQEAVAR
                     SLDGFEVFAKAHWGDHGLTSQITLYLRWIYEESLNQKDALAAFRERFRELQQHIPGFF
                     EALVLRADGTLAEGFDSIPELSPIMLTFGREYQRFRQGDPKPLQRRFVEYRSLFGPFV
                     SGSLEYATFTGIYLRSFSQIRSFFWHSGLRFDERSPFYPLVFISTREGFELLGLRVLR
                     LLLQRWLPTSRATIIDLERPLARWRLPAGISASQIRQSLLERRDHDRHPWFEQDHAWL
                     QIPCNQRYRLLVSL"
     gene            complement(866..>1646)
                     /locus_tag="PKO06_20360"
     CDS             complement(866..>1646)
                     /locus_tag="PKO06_20360"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV72071.1"
                     /translation="AARLLDQTSEDLQDLEVARTLQETFYPSSGVSVGEWRIWGRSRF
                     AGSVGGDYVDFFKVSEQQAILIIGDVSGHGIGAALVVAMARAAFAHPAVQGNPQRMME
                     SLNTLFVRTLKRRKMMSCCLAFVDAEQRTVTMVNAGHQWPLLVRNQRAEFLPTQGFPV
                     GAARNWSAGSRTLEVASDDVLFWYSDGLVEALDRQGISLGYKRLQDDLPSLCGNTPEE
                     TEGNVWRWFARLSPADPPEDDVSVLILQHQVVVGPRPGEVA"
BASE COUNT          369 a          520 c          470 g          287 t
ORIGIN      
        1 agactcacca gcaggcggta ccgttggtta cagggaatct gcagccaggc atgatcctgc
       61 tcaaaccacg gatgccgatc atgatcgcgg cgttcgagca ggctctggcg gatctgcgag
      121 gccgagatcc ctgccggcag tcgccaccgg gccagcggtc gttccaggtc gatgatggtc
      181 gcgcgtgacg tcggcagcca gcgttgcagg agaagccgca gcactcgcag ccccagcagt
      241 tcaaagcctt cgcgggtgga aatgaacacc agggggtaaa agggcgagcg ttcgtcgaac
      301 ctcagacccg aatgccagaa aaacgatcgt atctgagaaa aggatctcag gtagatgcca
      361 gtaaaggttg cgtattccag gcttccagag acgaaaggac caaacaatga ccggtactcc
      421 acaaagcgac gctgcagggg cttcgggtca ccctgccgaa accgctggta ctctcgcccg
      481 aaagtcagca tgatcggcga cagttccgga atggaatcaa agccctccgc caaggtccca
      541 tccgcccgga gaaccagggc ctcgaagaac ccgggaatgt gctgctgcaa ctctcgaaac
      601 cgctcgcgga atgccgccag cgcgtccttc tggttgaggc tctcctcgta gatccaccgc
      661 agatagagcg tgatctgact ggtgagcccg tggtcacccc agtgggcttt ggcaaacacc
      721 tcgaaaccgt cgagcgatcg cgcgacggct tcctgttcac ggtcatgctc ctgacgctcg
      781 aagaccagcc agatcagata cagcggcaga ccggtgaaca gcagaatcaa cagccatcgt
      841 cgcagttcac gtaccagggg agcagtcatg cgacctcgcc tggtcgcggc ccgaccacga
      901 cctgatgttg cagaatcagc acgctcacat catcctcagg cggatccgcc ggtgacaatc
      961 gcgcgaacca ccgccagaca ttgccctctg tttcttcagg cgtgtttccg cagagagagg
     1021 gcagatcgtc ctgcagtcgc ttgtacccaa gcgaaatccc ctgacgatca agtgcttcga
     1081 cgagaccgtc cgaataccag aagagcacgt cgtcactcgc gacttcgagc gttcgacttc
     1141 ctgccgacca gttacgggcg gcaccaacag gaaatccctg ggtcggcaga aactccgctc
     1201 tctggttcct caccagtagc ggccattgat gaccggcatt caccatcgtg acggttcgtt
     1261 gctcggcatc cacgaacgcg aggcaacagc tcatcatttt gcggcgcttg agagtgcgca
     1321 cgaacagggt attgagactt tccatcatcc gctgcggatt cccctgcacg gcagggtgag
     1381 caaacgcggc tcgagccatg gccaccacca gagcggcacc gatgccatgc ccggaaacat
     1441 ccccgatgat caggatggcc tgctgctctg aaaccttgaa gaagtcgaca tagtcgccac
     1501 ccacgcttcc ggcgaagcga cttctgcccc agatgcgcca ttcgccgacc gagaccccgg
     1561 acgacggata gaacgtttcc tgcagggtgc gcgccacttc gagatcctgc agatcttctg
     1621 aggtctggtc cagaagtcgg gcggcc
//
LOCUS       DAORHD010001228         1646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99164_length_1646_cov_2.462602, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001228 DAORHD010000000
VERSION     DAORHD010001228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99164_length_1646_cov_2.462602"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PKO06_20365"
     CDS             complement(<1..395)
                     /locus_tag="PKO06_20365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72072.1"
                     /translation="MRTRRAFTLIEIAIGVLIGSIVILAATKLLSGGLRSSTKGSSHL
                     SIIQATSILMCQLDEDLKRTVVIDFPALNAKDPTARLEILERTPSGILATSTITYQAG
                     PSGTGIVRRRDPTGGTASEHEFCRGFPVVP"
     gene            complement(392..826)
                     /locus_tag="PKO06_20370"
     CDS             complement(392..826)
                     /locus_tag="PKO06_20370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72073.1"
                     /translation="MMPRRAFTLTEVLMATIVLGLAMLPLFMVFSKGSAGTVQTRDEV
                     MGTGYVTELLALAQSLSYDDAFLDPKTKSPCQSLPLPSVAGETLGKPIDSRFKRYFTV
                     KEMTAGADVPYTYKVLIAEVDWLSNGISRSLQMTGMVYKGKP"
     gene            complement(823..>1646)
                     /locus_tag="PKO06_20375"
     CDS             complement(823..>1646)
                     /locus_tag="PKO06_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72074.1"
                     /translation="FPEYDGVQPAGITDYQKKWASGIGYMPFNSSLGFMFTNNRDAAP
                     WHHFGSDPILGFVKNEPADPDPALVNTIPDPFKAIDGVTALTSMQMLIEGGCLLKAGA
                     VGDRAAWVIDTAQESDVMKALGARGLLKNGKLDLNGWVVVRGNGQIKIDQNLELASNG
                     GLYLENGNITISRAINGSGKTLQLVAANGDIVIDFSGQVDAALVAKGSVLTQNAKASI
                     KGAVASGKFPLSNCQQEVRVEFDRKLAAVPNAAGSDPTEEELLGIAFEPQPILIQ"
BASE COUNT          363 a          501 c          443 g          339 t
ORIGIN      
        1 ggcacgaccg gaaacccgcg acagaactcg tgttcactcg ccgtaccacc cgtgggatca
       61 cgacgtctga cgatgccggt acccgacggc ccggcctgat aggtaatggt cgaagtggca
      121 aggattccgg agggggtgcg ttcgagaatc tcgagtcgcg ccgtcgggtc tttggcgttc
      181 agtgccggaa agtcgatgac gaccgtgcgt ttcaggtctt catcgagctg gcacatcaga
      241 atcgaagtag cctggatgat gctcagatgc gaactgccct tggtcgaact gcgcaggcca
      301 cccgacagca gcttggttgc tgccagaatg acgatcgacc cgatcagcac cccaatcgca
      361 atttcgatga gcgtaaaggc gcgtcgcgtt ctcatggttt gcctttatag accatgccgg
      421 tcatctgcag actgcgcgag atgccattgc tgagccagtc aacttcggca atcagcacct
      481 tataggtata gggaacatca gcgcccgccg tcatttcctt cacggtaaag taccgtttaa
      541 atcgcgagtc aattggcttg ccaagcgttt caccagcaac agaaggcaag ggaagactct
      601 gacacggcga cttcgtcttg ggatcaagaa acgcatcatc ataggacaaa ctctgcgcca
      661 acgccaacaa ctcggttacg tatccggtac ccatcacctc atctcgagtc tgtaccgtac
      721 ccgctgaccc cttggaaaat accatgaaca gaggcagcat ggccagacca aggacaatgg
      781 tcgccatcag cacttccgtg agggtaaagg cacgacgagg catcattgaa tcagtatcgg
      841 ctgcggctcg aaggcaatgc cgagcagctc ttcctcagta ggatctgacc cagcggcatt
      901 cggcaccgcg gccagttttc ggtcaaactc aacccggact tcctgctgac agttcgacag
      961 cggaaacttg ccggaagcca ccgcaccttt gatcgatgcc ttggcatttt gcgtcagcac
     1021 tgaacccttg gccaccagcg cggcatcgac ctggccgctg aagtcaatga cgatatcgcc
     1081 attggccgcc accaactgca gggttttgcc gctgccattg atcgcccgcg agatcgtgat
     1141 gttcccgttc tcgagataca ggccgccgtt actggccagt tcgaggttct gatcgatctt
     1201 gatctgaccg ttgccgcgca ccacgaccca cccgttcagg tcgagcttgc cgttcttcag
     1261 caaaccacga gcacccagag ccttcatgac gtctgactct tgtgccgtat cgatgaccca
     1321 ggcggcacga tcgccgacag ctccggcttt gagcaggcac ccgccctcga tcaacatctg
     1381 catcgaggtc agcgccgtca ccccatctat cgccttaaac gggtcaggaa tcgtgttcac
     1441 cagggccggg tccggatcag caggctcgtt tttgacgaac ccgagaatgg ggtcgctgcc
     1501 aaaatggtgc cagggtgccg catcgcggtt gttcgtaaac atgaagccca gcgatgaatt
     1561 gaacggcatg tatccgatcc cgctcgccca cttcttctga taatccgtga tgccggccgg
     1621 ctgaacgccg tcgtattcag gaaaca
//
LOCUS       DAORHD010001229         1644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99324_length_1644_cov_3.154185, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001229 DAORHD010000000
VERSION     DAORHD010001229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99324_length_1644_cov_3.154185"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1644
                     /gene="lon"
                     /locus_tag="PKO06_20380"
     CDS             <1..>1644
                     /gene="lon"
                     /locus_tag="PKO06_20380"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV72075.1"
                     /translation="LFVGRKKSIRAIEEAMIRDRKIILVSQKQAKTDDPAVEELNLTG
                     TVAEVLQLLKLPDGILKILVEGTSRVRIKNFVKNDEFFEVEAEDVVSSDEKTIEVQAL
                     MRNVINLFEQYVKLNKKIPLEVIMVVNNVEEPGRLADIITAHLSLKVEEKQEILEAFE
                     SRARLEKIAGILNRELEIMMVEKKIRGQVRKQMEQIQKEYYLREQMKAIQKELGEGDE
                     RTEELDELKEAIAKAKMPDDARQKAEKELSKLAKMPPGSAEATVSRNYIDWLVALPWA
                     KSSKDQIDLEQSEKILDEDHFGLKKVKERILEYLAVCKLKKSIKGPILCLIGPPGVGK
                     TSLGRSIARSMNRKFARISLGGMRDEAEIRGHRRTYIGAMPGRIIQALRDCGTRNPVI
                     LLDEIDKLGMDFRGDPSSALLEVLDPEQNYSFSDHYVSTPFDVSKVLFLTTANVPHTI
                     PGPLRDRLEIVFLPGYTEEEKLEIAKRYLMPKQREANGLEKFKVEFEEETVRQIIRKY
                     TRESGVRSLEREIASVCRKVARKMVQIKRKAEDGSEADK"
BASE COUNT          429 a          418 c          477 g          320 t
ORIGIN      
        1 ccttgtttgt cggccgcaaa aagtcgattc gggccatcga ggaggccatg attcgtgatc
       61 gcaagatcat tcttgtttca cagaagcagg ccaagacaga cgatccggcc gttgaagaac
      121 tcaacctgac aggtactgtt gccgaagtgc tgcagctgct gaagttgccc gacgggatcc
      181 tcaagattct ggtcgagggc accagtcgcg tgcgcatcaa gaacttcgtc aaaaatgacg
      241 aatttttcga agtcgaagcg gaagacgtgg tcagctctga tgagaagacc atcgaagtgc
      301 aggcgctgat gcgcaatgtg atcaatctct tcgaacagta cgtgaaattg aacaagaaga
      361 ttccgctcga agtgatcatg gtcgtgaaca acgtcgagga accagggcgc ctggctgaca
      421 tcatcacggc tcacctcagt ctgaaagtcg aagaaaagca ggagattctc gaagcgttcg
      481 agtctcgggc ccgactcgag aagattgccg gcattctgaa tcgcgaactc gagatcatga
      541 tggtggaaaa aaagattcgc ggccaggttc gcaagcagat ggagcagatt cagaaagagt
      601 actacctgcg cgaacagatg aaggctattc agaaagaact tggcgagggc gatgaacgca
      661 ccgaagaact cgatgagctg aaggaagcca ttgccaaggc caagatgcca gacgatgccc
      721 gccagaaagc ggaaaaggaa ctgagcaagc tcgcgaagat gccgcccgga tcggctgaag
      781 cgaccgtatc gcgcaactat atcgattggc tggttgctct gccgtgggcg aagtcctcga
      841 aagatcagat tgatctcgaa cagtctgaaa agattcttga cgaagaccat tttggcctga
      901 agaaggtgaa agaacgcatt ctcgagtatc tcgcggtctg caaactgaaa aaatcgatca
      961 aaggcccgat tctgtgcctg atcgggcctc ccggggtggg caagacctcg ctgggccgct
     1021 cgattgcccg ttcgatgaac cgcaagttcg cccgcatttc actgggcggc atgcgggatg
     1081 aagcggaaat tcgcggccac cggcggacct atatcggtgc catgccgggg cgcattattc
     1141 aagcgctgcg cgactgtggt acgcgcaacc cggtgattct gctcgatgaa atcgacaagc
     1201 tgggcatgga tttccgtggt gaccccagtt cggcattgct cgaagtgctt gatccagagc
     1261 agaactactc gttttctgac cattacgttt cgaccccctt cgatgtttcc aaggtgctgt
     1321 tcctgacgac tgccaacgtc ccacacacga ttccgggacc actgcgcgat cgtctcgaga
     1381 tcgtctttct gcccggatac accgaagaag agaagctcga aattgccaag cgctatctga
     1441 tgccgaaaca gcgtgaagcg aacggcctcg agaagttcaa ggtggagttc gaggaagaaa
     1501 ccgtgcggca gatcattcgc aaatacaccc gcgagtccgg ggtacgcagc ctcgaacgtg
     1561 agattgcgtc cgtgtgtcga aaagtggcgc gcaagatggt gcagatcaag cgcaaagccg
     1621 aagacggcag tgaggccgac aaga
//
LOCUS       DAORHD010001230         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99507_length_1642_cov_3.459357, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001230 DAORHD010000000
VERSION     DAORHD010001230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99507_length_1642_cov_3.459357"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="PKO06_20385"
     CDS             <1..1441
                     /locus_tag="PKO06_20385"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV72076.1"
                     /translation="FKRLPITHAHEFPRLMQAAGVSPLPADRVKLIESQFQTVVPEGT
                     SVRRDSCSLAHVAFEFERPKVFSVPFAEKDCPVTGTLLNFELANGPNWFALQKDNGTF
                     VGSLCSILENRLPQPPEVDLRTKALRRRLQLLAAAFERDQARPLRERVNDLRLIPAVI
                     GGGTRYDQAVRISRRAKALGERLQIAVSEIAIDQTAFFTFCGNTLHLSRLLPSRVVAI
                     VALLASLQDVPAEVRDALQDLGQCGLGTDTLPSTSVGCLIAAGWLLDQILVLPESATP
                     ERERRSGELLRLALGRKWNLDLLALTIEPKDAVLPREFWEARLAQSLESVGFAFERHG
                     RLLKNVCLDPTGLAETIKGWPDGGGKEMDLVTVLARKVVARCGTRAQSLPVSLPGSAQ
                     EESLDVKLKEAEGIWPGRAKALTDLLWAARADVEACMMDLPPVLEEQDTAWNSRLALL
                     MRFVRLAARLPGVSGLESSKLEVQKHGET"
BASE COUNT          304 a          463 c          515 g          360 t
ORIGIN      
        1 gttcaagcgg ctgcccatca cgcatgcaca tgaatttccg cgtttgatgc aggccgcggg
       61 tgtctcgcca ctgccggcag atcgtgtgaa actgattgaa tcgcagtttc agacagttgt
      121 tcccgaggga accagtgtgc gacgggattc gtgcagtctc gctcatgtgg cgttcgagtt
      181 tgagcgcccg aaagtctttt cggtgccatt tgccgagaaa gactgcccgg tgacgggcac
      241 gcttctgaac tttgaattgg cgaacggacc gaactggttc gccctgcaga aggacaacgg
      301 aaccttcgtc ggatcgctct gcagcattct cgagaatcgc ttaccgcaac cgccggaagt
      361 cgatctgcgc accaaggctc tgcgtcggcg tttgcaactc ctggctgcgg cttttgagcg
      421 cgatcaggcg cgaccgctgc gcgagcgggt gaatgatctg cgattgattc cggcggtaat
      481 cggtggcggg actcgatatg atcaggcagt gcgcatttct cgacgagcca aggctctcgg
      541 agagcgactg cagatcgcgg tcagcgagat cgccatcgat cagaccgcct ttttcacgtt
      601 ctgcggcaac acgctgcacc tcagccgcct gctgccttca cgggtcgtgg cgatcgtggc
      661 tttgttggct tcactgcagg atgttcctgc ggaagtgcgt gacgcgctgc aggatcttgg
      721 tcagtgcggt cttggcaccg atacgttgcc ctccacctcg gtggggtgtc ttattgccgc
      781 cggctggttg ctcgatcaga ttctggtgtt gccagagagc gcgacgccgg aacgagagcg
      841 acggagcgga gaactgcttc gtctggccct gggacgcaaa tggaatctcg acctgcttgc
      901 cctcacgatc gaacccaagg atgccgtgct tccccgtgag ttctgggaag ctcgactggc
      961 ccagtctctc gagtctgtcg gcttcgcatt cgagcgtcat gggcgtctgc tcaagaatgt
     1021 ctgccttgat ccgacaggat tggccgagac gattaaggga tggcctgacg gtggcggcaa
     1081 ggagatggat ctggtgacgg ttctggcgcg caaagtggtc gctcggtgcg ggacgcgggc
     1141 acagagcctg ccggtttcgc tgcccgggtc ggcgcaggaa gaatctctcg atgtgaagct
     1201 caaagaagcg gaaggaatct ggccggggcg cgcgaaagcg ctgaccgatc tgctgtgggc
     1261 ggctcgagct gacgtcgagg cgtgcatgat ggatctgccg cccgtgctgg aagaacagga
     1321 cacggcctgg aacagccgtc tggcgctgct catgcgattc gttcgactgg ctgctcgact
     1381 cccgggtgtt tccggccttg aatcctcgaa gctcgaagtt cagaagcacg gagagacgtg
     1441 atatttccct ttttagacaa gaatatggta aaaccctgtc gaatgcctgt accgtacgcg
     1501 cacaggtatg tgcgaccgta cggttaatcc tgaaggagga aacccctttc ccatgatcgc
     1561 tgcagaagat aaatccaccc ctggcggaac cctcccccct tctcgcacgc tttttccgtt
     1621 gctgccacta cgcgatgtgg tc
//
LOCUS       DAORHD010001231         1639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99822_length_1639_cov_3.072601, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001231 DAORHD010000000
VERSION     DAORHD010001231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99822_length_1639_cov_3.072601"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PKO06_20390"
     CDS             <1..205
                     /locus_tag="PKO06_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72077.1"
                     /translation="TYLRTFSDHAAVNAVSGFDTTNLPFFRQDAEVVLAFFTIFFIAG
                     TRTSAVPYYRISPSKPSGRRRYS"
     gene            304..999
                     /locus_tag="PKO06_20395"
     CDS             304..999
                     /locus_tag="PKO06_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV72078.1"
                     /translation="MLKNATEQRRRLSFLPLLAVLAIALAGCTDTAGKSSDVNPLAAY
                     QASGTTANGPTNGQTQGVGVIDQPIVSVPGPNDSTNGHSNAPLLDNWHPGWQQSDCFS
                     CHTDQSRIPDHNYPDTANCYLCHGTNGLPGFGDNTPPVIKGIVPTPTKNSVTISWSTD
                     ELAISKLILRTKDGDRMDFPVSTDYVTSHRYSVPGLLPNTTYTFEIQCTDKAHNTGTT
                     ATIGMFQFTTLAS"
     gene            1081..>1639
                     /locus_tag="PKO06_20400"
     CDS             1081..>1639
                     /locus_tag="PKO06_20400"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HNV72079.1"
                     /translation="MGWFSRLTGGDKKTSLPADLWKKCPRCSEVLINKELPRNLFICP
                     KCDHHLRMPIWERIALLTDDTSFVELDRTLESLDPLNFVDSTPYPDRLRKAIEATDYF
                     EAVTCGQGRIEDLPIMLGVMNFDFMGGSMGSVVGEKITRLFETAIEKRLPVVVISASG
                     GARMQEGIFSLMQMAKTSQATARLAE"
BASE COUNT          341 a          528 c          422 g          348 t
ORIGIN      
        1 aacctacttg cgaactttct cagaccacgc cgccgtcaac gccgtttccg gcttcgacac
       61 gacgaatctt ccgttctttc ggcaggatgc ggaagttgtt ttagctttct tcacaatttt
      121 cttcatcgcc ggtacgcgca cgagtgccgt tccctattat cgcatttccc cttcgaaacc
      181 ttctggaaga cgccgatatt catagagatt acgccaggtg tcagactcag tcgcaggtca
      241 tcaccgctct gcatcagaat ctgaggccgg tctggcggaa ccgtttcatc gggaggcagt
      301 atcatgttga agaatgcaac cgagcagcgc cgacggctct ctttcctgcc acttctcgcc
      361 gtcctggcaa ttgcgctggc aggctgtacc gacaccgccg gcaagtcgtc tgacgttaac
      421 cctctggcgg cctatcaggc ctcgggcacg accgcgaacg ggcccaccaa cggccagacg
      481 cagggcgtcg gtgtgatcga tcagcccatc gtctcggtgc cgggtcccaa tgacagcacc
      541 aacgggcaca gcaacgcccc ccttctggac aactggcacc cggggtggca gcagtccgat
      601 tgtttcagct gtcacacaga ccagagccgc attccggatc acaactaccc ggacaccgcg
      661 aactgctacc tctgccacgg cacgaatggt ctgcccggct tcggtgacaa tacccccccg
      721 gttatcaagg gcattgttcc gacgcccacg aagaactccg tgactatttc ctggtccacg
      781 gacgaactgg cgatctcgaa gctgattctg cgtaccaaag acggcgatcg catggatttc
      841 ccggtctcga ctgactatgt gaccagccac cgatactcgg ttcccggtct gctgcccaac
      901 actacctata cttttgagat tcaatgtacc gacaaggctc ataacacagg gacgacggcc
      961 accatcggga tgttccaatt cacgacgctc gcctcgtaac cgccctgcgc ccggattgaa
     1021 aaccctccgg aattctgcta taacaagcac caatcgattc cggggggttc tttcttttcg
     1081 atgggttggt tttcacgtct gacgggtggc gacaagaaaa cgtcgctgcc ggccgatctc
     1141 tggaaaaagt gcccgcgctg ctctgaagtt cttatcaata aagagcttcc ccgcaatctg
     1201 ttcatctgcc ccaagtgcga tcaccatctg cgcatgccga tctgggaacg gatcgcgctg
     1261 ctcactgatg acacctcgtt tgtcgagctc gatcgcacgc tcgagtcgct cgaccccctg
     1321 aattttgtcg attccacccc ctaccccgac cgcctgcgca aagcgatcga ggccaccgac
     1381 tacttcgaag cggtgacctg cggacaggga cgcatcgaag atctgcccat catgctcggc
     1441 gtcatgaact tcgatttcat gggcggcagc atgggttcgg tcgtcggcga aaagattacc
     1501 cgcctcttcg agacagctat cgaaaagcgg ctgccggtgg tcgtcatctc ggcctcaggg
     1561 ggcgcacgca tgcaggaagg catcttctcg ctgatgcaga tggccaaaac ctcgcaggcc
     1621 acggccagac tcgcagaag
//
LOCUS       DAORHD010001232         1638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_99942_length_1638_cov_3.038534, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001232 DAORHD010000000
VERSION     DAORHD010001232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99942_length_1638_cov_3.038534"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PKO06_20405"
     CDS             complement(<1..875)
                     /locus_tag="PKO06_20405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72080.1"
                     /translation="MTRQLLAFARKQTVTPRVLDLNDTVEGMLKMLRRIIGEDITLTW
                     RPGKNLWPVCMDPVQIDQILANLCVNSRDAISGTGRVTIETGMTTLDQASATVSTGIT
                     PGDFVYLAISDDGCGMDAATVSHLFEPFFTTKELGKGTGLGLATVYGIVRQNNGGINV
                     YSEPGRGTTFKVYLPRHRGVFEERSLKTSPEKMKTGNELVLLVEDEAAILKLTERMLK
                     QLGYRVLAATTPSAAFRLVDEQQGAVQLLVTDVIMPEMNGRELREQLNQKYPKLRTLF
                     MSGYTSDHVQIQEIL"
     gene            complement(882..>1638)
                     /locus_tag="PKO06_20410"
     CDS             complement(882..>1638)
                     /locus_tag="PKO06_20410"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV72081.1"
                     /translation="LAAVEAHLSGKTSTFDIVFRFRTKNDDWMWIRGRGRIIERDAGG
                     KPIRMIGTHTDCTKQMRAEMALRKSEERFQKMLGDVPDMVSIHDCDMNVLYSNWQGYA
                     SVPADRRVCGDKCYRIYRGRDTQCPDCLAQKVFDTGKPVQTEAHLPDGRWVDLRVIPL
                     LDDQGRVELFVEWVRDITDQKKALEEREKLQSQLAQSQKMESIGRLAGGVAHDFNNML
                     GVILGHTDLILSRFPPDDQSHADLQEISKAAER"
BASE COUNT          338 a          491 c          415 g          394 t
ORIGIN      
        1 ttgagtattt cctggatctg gacatgatcg gacgtgtatc cggacataaa cagcgtacgc
       61 agcttgggat acttctggtt caattgctct ctcaattcac gaccgttcat ctcaggcatg
      121 atgacgtcgg tcaccagcag ctgcaccgcc ccctgctgct cgtcgaccag tcgaaatgct
      181 gcactgggcg ttgtggcagc cagaacccga tagcccagtt gcttcaacat cctctccgtc
      241 agcttgagaa tcgccgcctc atcttcaacc agaagtacaa gttcatttcc ggttttcatc
      301 ttttctggcg atgttttcaa agatcgttct tcaaaaacac cacggtgccg cggcaggtat
      361 accttaaagg tcgtgccccg accgggctca ctgtagacgt tgatcccgcc gttgttctgc
      421 ctgacgatgc cgtacacggt cgccaaccca agcccggttc ccttgcccag ttccttggtg
      481 gtgaagaacg gttcgaacag gtgactgacc gttgcagcat ccattccgca gccatcatca
      541 ctgatcgcca gatagacaaa atcgccgggg gtgatccctg ttgagaccgt tgcactagcc
      601 tgatcaagcg ttgtcatgcc ggtttcaata gtgacccgac cggtacccga aatggcatct
      661 cgggaattga cacacaaatt ggccagaatc tggtcgatct gcaccggatc catgcacacc
      721 ggccagaggt tctttcccgg tcgccaggtg agcgtgatgt cttcgccgat gatgcgacgt
      781 aacatcttca gcattccctc aaccgtgtcg ttcagatcga gaacccgggg agtcacggtc
      841 tgtttgcggg caaaggccag caactgcctt gtcaatgctg cttaccgctc cgccgccttg
      901 ctgatttcct gaagatcggc atgcgattga tcatctggag gaaatctcga taggatcagg
      961 tcagtatgtc caaggatgac tcccagcatg ttattgaaat catgcgccac gccgccggct
     1021 aaacgaccga tcgattccat tttctgcgac tgagccagct ggctctgcaa cttttcccgt
     1081 tcctcgagcg cttttttctg gtcggtgata tctcgcaccc actcgacgaa caactcgacc
     1141 cgcccctgat cgtcaagcag ggggatgacc cgcagatcga cccagcggcc atccgggaga
     1201 tgggcctcgg tttgcaccgg tttgccggta tcaaaaacct tttgtgccag gcaatccggg
     1261 cactgggtat cacgaccacg atagatccga taacacttgt cgccgcagac gcgtcgatct
     1321 gcggggaccg aggcatagcc ctgccagttg ctgtacagca cgttcatgtc gcaatcgtgt
     1381 atcgacacca tatcagggac gtccccaagc atcttctgaa atcgctcttc gctctttctt
     1441 agagccattt ccgctcgcat ctgcttggta caatcggtgt gtgttccgat catacgtatc
     1501 ggctttcccc cggcgtcgcg ttcaatgatg cgacctcggc cgcgaatcca catccagtca
     1561 tcgttctttg ttctgaagcg gaagacgatg tcgaaggtcg acgtcttgcc ggacagatgc
     1621 gcctcgaccg ctgcgagg
//
LOCUS       DAORHD010001233         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100019_length_1637_cov_3.478508, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001233 DAORHD010000000
VERSION     DAORHD010001233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100019_length_1637_cov_3.478508"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1637)
                     /locus_tag="PKO06_20415"
     CDS             complement(<1..>1637)
                     /locus_tag="PKO06_20415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV72082.1"
                     /translation="WYSMLGYQPNEFPASFEAWRELLHPEEQESIPQKVMAGASTAPF
                     SFTLEFRLRCRNGKHLWIRAKGKAVEFDTTGKAVRLIGTHTDIQTEKEHEQESHLLFS
                     ALEAAPINFVITDADGVIEYVNAEFVRITGYSRPEALGQNPRIIKSGKHDALFYRTMW
                     QRLTNGEVWRGLLINKRKDGTLYEEDKTIAPVCDKSGRPKFYISASRDVTRERLVEKQ
                     LQQAQKMQALGTLAGGIAHDFNNILMAIQGYAELALEEGGDNPQIKKNIDQVITGASR
                     ASKLIEQILTFSRQRETDLQSIDPVPIFKETIKFLRGSLPSIIQMRQELEPDCGPILA
                     DPTQIHQIMTNLCSNAHHAMREKGGRLTISLKRVDRVPEHPCHVQHSLECPEGFACLA
                     VEDTGCGIPPELQARVFEPFFTTKPQGEGTGMGLSTVQGIVYAAGGHIILTSELGKGT
                     RVEVYLPRLQARSLSENREDTRIVGGHERILIVDDEEHVVDLLKSLLEKWGYSVTTCY
                     NGQEALLRFEVDQKAYDLVLTDQSMPGMTGIQLAKRL"
BASE COUNT          325 a          476 c          439 g          397 t
ORIGIN      
        1 gcagtcgctt ggcgagctgt ataccggtca tgccaggcat cgactggtcg gtcagcacca
       61 gatcatacgc cttctggtcc acctcgaatc gcagcaaagc ttcctgccca ttataacagg
      121 ttgtcaccga atagccccac ttttcgagca aacttttcag cagatccacg acatgctctt
      181 cgtcatcgac aatcagaatt cgttcatggc ccccgacaat gcgcgtgtct tcacgattct
      241 cgctcaggct gcgcgcctgc aggcgtggca gatacacttc cactcgcgtt cccttcccca
      301 gttcactggt cagaatgatg tgcccgcctg ccgcgtagac gatgccctga accgtcgaca
      361 atcccatgcc tgttccctct ccctgcggtt tcgtggtgaa gaatggctca aacacgcggg
      421 cctgcagttc ggggggaatc ccacacccgg tgtcttcgac cgccaggcac gcgaatccct
      481 cggggcattc gagcgaatgc tgaacatgac aggggtgttc gggaacacga tcaacgcgct
      541 tgaggctgat ggtcaggcgt ccgcccttct cgcgcatggc atgatgtgca ttggaacaaa
      601 ggttcgtcat gatctgatga atctgggtcg gatcggcaag gatcggaccg cagtccggtt
      661 caagctcctg tcgcatctga atgatcgacg gcaggcttcc gcgcagaaac ttgatggtct
      721 ctttaaagat cgggacagga tcgatcgact gcagatcggt ctcacgctgt ctcgagaacg
      781 tcagaatctg ttcgatcagc ttgctggcac gagaggcgcc ggtgatgacc tgatcgatgt
      841 tctttttgat ctgggggttg tcaccccctt cctcgagcgc gagttcggca tagccctgaa
      901 tcgccatgag aatattgttg aaatcatgcg cgatgccgcc ggcgagcgtc ccgagtgcct
      961 gcatcttctg ggcctgctgc agctgctttt cgaccagacg ttcacgcgtg acatcgcgag
     1021 aagcgctgat atagaacttg ggtcgccctg acttgtcgca gacaggggcg atcgttttat
     1081 cttcttcgta caaggtgcca tctttacgtt tgttgatcag cagtcctctc cagacctcac
     1141 cattcgtcag tcgctgccac atagtgcgat agaacagcgc atcatgcttg ccgcttttaa
     1201 tgattctcgg gttttgtccc agtgcctcgg ggcgagaata cccggtaatt ctgacaaatt
     1261 cggcattcac atactcgatc acgccgtcgg cgtccgtgat gacaaagttg atcggcgcag
     1321 cttcgagggc gctgaataac aggtgacttt cctgctcgtg ttccttttct gtctgaatgt
     1381 cggtgtgcgt cccgatcagg cgtacagcct ttccggtcgt atcaaactcc accgcttttc
     1441 ctttggctct gatccagaga tgtttcccat tgcggcagcg caggcggaac tcgagggtaa
     1501 agctgaacgg cgcggtcgaa gcccccgcca taaccttctg cggaatcgac tcttgttctt
     1561 cgggatggag cagttctctc caggcttcga acgaagccgg aaactcattc ggctgatagc
     1621 cgagcatcga ataccag
//
LOCUS       DAORHD010001234         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100439_length_1633_cov_3.667300, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001234 DAORHD010000000
VERSION     DAORHD010001234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100439_length_1633_cov_3.667300"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKO06_20420"
     CDS             complement(<1..531)
                     /locus_tag="PKO06_20420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72083.1"
                     /translation="MDVTAALSLLPGALLGGIGAALATAWLVSRHFHRRLAEEARTSD
                     LERFHCQKTAEAFVEREKHFREFVEFLPQAVIETDLDGKFIYINQKAGELLRFTPVDL
                     ATGVSWVSICHSSHLDRLWRYLKDGTDGGTGDEYLLVKKDGTTFPALLYCHRVLLSGA
                     GVGFRFVLVDISARRHI"
     gene            850..1221
                     /locus_tag="PKO06_20425"
     CDS             850..1221
                     /locus_tag="PKO06_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72084.1"
                     /translation="MKNRLDIDTWEEAFTRLCHEKLDEEPLYEYEHLYKKGLSPEQAF
                     MTYLKENPDYLEKLQEILEEQKRQEAKKLPPEELARREAEQQRRDEAKKRLSQYCPNC
                     ARLMAGKKQCKCGYKRGKDEQ"
     gene            1218..>1633
                     /locus_tag="PKO06_20430"
     CDS             1218..>1633
                     /locus_tag="PKO06_20430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72085.1"
                     /translation="MTARTRTRKTLSPSSQGTPSKRTTRLITADDLRRFHLIGNQQIS
                     PDATQILFVKRHVEEPNKQISNLWLVDTKSGKSRQFTTGNKDAMGFWSPCGRKVAFVS
                     GREKGKAQIYLISAEGGEARALTKFPQGSIAGFEWAP"
BASE COUNT          402 a          494 c          448 g          289 t
ORIGIN      
        1 gatgtgtcga cgagcactga tgtcgacgag cacaaagcga aaacccactc ccgcgccact
       61 cagaaggacg cggtgacagt agagcaacgc ggggaacgtc gtaccgtctt ttttcaccag
      121 aaggtattca tctccggtcc cgccgtcggt cccatccttc aggtagcgcc acaggcgatc
      181 gagatgggat gaatggcaga tgctcaccca gctcacgccc gtcgccagat cgacaggcgt
      241 gaaccgcagc aactcaccgg ccttctgatt gatgtatatg aactttccgt caagatcggt
      301 ctcaatgacc gcctgaggca gaaactcaac aaactcgcga aaatgctttt cgcgctcgac
      361 gaacgcttct gccgtcttct ggcagtgaaa ccgctcgagg tcagaggtgc gcgcctcttc
      421 cgccagacgt cgatgaaagt ggcgactcac cagccaggcc gtggcaagcg ctgcgccgat
      481 cccgcccaac agcgcgcctg gtaacaggga cagcgcggca gtcacatcca taccgccccc
      541 caatctaagg tgtcattcca cattgtagcc tctcagatca tcattggcaa aaaggttttc
      601 ctctggggca attcgttctc accccgagtc gaggggcgca ctctctgccg caacggcgaa
      661 tcgttgagcg atggcaacga agaacagtct tgggagagca tggaatggtc cgtcggctct
      721 ggtgtcgact ttacaacaag aggcgtggaa tgctagtctc atgcgatcat tccccgccgc
      781 ggggcgacgg acatctctca accgcgccgt tcacggcaca cgggaacctc gcacaagaga
      841 ggatcgtgta tgaagaatcg tctcgatatc gacacctggg aagaagcatt cacccgtctg
      901 tgtcacgaaa agcttgatga agagcctctg tatgagtatg agcacttgta caaaaaggga
      961 ctgtcgccgg agcaggcctt catgacctat ctcaaggaaa atccggacta cctggaaaaa
     1021 ctgcaggaga tcctggagga gcagaagcgg caggaagcca agaaactgcc gcccgaggaa
     1081 ctcgctcgac gtgaggccga acagcaacgt cgcgacgagg caaagaaacg tctcagtcag
     1141 tattgtccca attgcgcccg gctcatggcg ggcaagaagc agtgcaaatg cggatacaaa
     1201 cgcggaaagg atgaacaatg accgccagga cccgaacccg caaaacgctt tcccccagct
     1261 ctcagggcac tccctcgaag cgcaccacgc gcctgatcac cgccgacgat ctgcgacgct
     1321 ttcatctcat cggcaatcag cagatctcac ccgatgccac ccagatcctg tttgtcaaac
     1381 gtcatgtcga agagccaaac aagcagattt ccaacctctg gctcgtcgac acgaagtccg
     1441 ggaaatcgcg ccagttcacc acggggaaca aagatgccat gggcttctgg tctccctgcg
     1501 gacgcaaggt ggccttcgtc agcgggcgcg agaaaggcaa agcccagatc tacctgatca
     1561 gtgcggaagg cggcgaagcc cgagctctca ccaagtttcc ccaggggtcg attgccgggt
     1621 tcgagtgggc ccc
//
LOCUS       DAORHD010001235         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100561_length_1632_cov_2.887127, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001235 DAORHD010000000
VERSION     DAORHD010001235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100561_length_1632_cov_2.887127"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="PKO06_20435"
     CDS             complement(<1..1420)
                     /locus_tag="PKO06_20435"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV72086.1"
                     /translation="MTFNRYLIGIVLVILFMVMTFASALYHTFFVYVPADKMLIIISK
                     SGEELPAGQVIASRPEQKGIWKDPLGEGRHFVMPYLYETQLKDVVTIKPGQVGVVTSR
                     VGKTASPGAILVNDDEIGIWRRVLTPGKYRLNPYAYKVDIHPAVAIQPGFVGFVTSLV
                     GREADGMFAGDGEKGIRKNVLHPGLYFINPYEFKVTEVEVGVNQESFLDENQITFPSK
                     DAFSIAVEATVEWELLPEHVAQVMSEFGGPKAVVEKIIVPQSKSIGRIQGSSYSAKEF
                     LLGVEREKFQKTFTLELERICEAKNITIHSAFIRNLSIPDSLLTPIREAFVAVEQEKT
                     AKVWEDTRKSAMELEREQARITQRRQQVIAETQAAIRTIEAETAQEVGKIEASTRLEV
                     ANVEQQIALIEAEKTKVLGEAQATVAQLEGEAKSKGLESKIRAFGDNPSAYVNYSFAR
                     RLPDDLKLQLIYSGEGTLWTDLQ"
     gene            complement(1422..>1632)
                     /locus_tag="PKO06_20440"
     CDS             complement(1422..>1632)
                     /locus_tag="PKO06_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72087.1"
                     /translation="ADVALMQANAEANALKKSIEAFKSPEHFASYEFLIRVGPSIQSI
                     FANTEGMFGRIFSGLIPAEAQKGGK"
BASE COUNT          318 a          468 c          474 g          372 t
ORIGIN      
        1 tctgcaggtc ggtccagagc gttccttcgc ccgagtagat cagttgcagc ttgagatcgt
       61 ctggcagacg gcgtgcgaac gagtagttca cgtaggccga ggggttgtcg ccaaacgccc
      121 gaatcttcga ttccagaccc ttcgatttcg cctcaccttc gagctgagcg accgtcgctt
      181 gcgcttcgcc gagcaccttg gtcttctcgg cctcgatcag cgcgatctgt tgttcgacgt
      241 tcgcgacttc gaggcgggtc gaggcttcga ttttgccgac ttcctgcgcg gtctcagcct
      301 cgatggtgcg aatcgcagcc tgcgtctcgg cgatgacctg ctgccgacgc tgggtgatgc
      361 gcgcctgttc ccgctcaagt tccatcgcgg acttgcgtgt gtcttcccag accttggctg
      421 tcttctcctg ctcgacggcg acaaacgctt cgcggatcgg cgtcaacaga ctgtccggaa
      481 tcgacaggtt ccggatgaac gcgctgtgaa tcgtgatgtt cttcgcttcg cagatgcgct
      541 cgagctcgag agtaaatgtc ttctgaaatt tctcgcgctc gacgccgagc agaaattcct
      601 tggcgctgta ggaagaacct tgaatgcgtc cgatcgactt gctctgcggc acgatgatct
      661 tctcgacaac ggccttgggc ccgccgaact cactcatgac ctgcgcgacg tgctccggca
      721 gcagttccca ttcgacggtc gcttcgacgg caatgctgaa cgcgtctttc gaagggaagg
      781 tgatctgatt ctcatcgagg aaactctctt gattgacgcc gacctcgact tcggtcactt
      841 tgaattcgta cgggttgatg aagtacaaac cgggatgcag aacgttcttc cgaataccct
      901 tttctccatc accggcaaac atgccgtcgg cttcgcgccc gacgagtgag gtgacgaatc
      961 cgacaaaccc gggttgaatg gccacggccg ggtggatgtc gaccttgtag gcatacggat
     1021 tcagtcgata tttgccagga gtcagcacgc gtcgccagat gccgatctca tcgtcgttga
     1081 ccagaatggc tccgggcgag gcggtcttgc cgacgcgaga ggtgacgacg ccgacctggc
     1141 cgggcttgat ggtgacgaca tccttgagct gagtctcata caggtagggc atcacgaaat
     1201 gccggccttc acccagcgga tccttccaga tacctttctg ctcaggtcgg ctggcgatga
     1261 cctgtccggc gggaagctcc tcgccggact tcgagatgat gatcaacatc ttgtcggcgg
     1321 gaacatagac gaagaacgtg tgatacagcg cggaagcgaa agtcatgacc atgaacagaa
     1381 tgacgagaac aatgccgatc agatagcggt taaaagtcat ctcacttgcc tcccttctgg
     1441 gcttcagccg ggatcaggcc gctgaagatg cgcccgaaca tgccttcggt gttggcaaag
     1501 atgctctgaa tcgatggccc gactcgaatc agaaactcgt atgaggcgaa gtgttccggg
     1561 ctcttgaacg cctcgatgga cttcttgagg gcattggctt cggcatttgc ctgcatgagt
     1621 gcgacatcgg cg
//
LOCUS       DAORHD010001236         1631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100664_length_1631_cov_2.921954, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001236 DAORHD010000000
VERSION     DAORHD010001236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100664_length_1631_cov_2.921954"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1631)
                     /locus_tag="PKO06_20445"
     CDS             complement(<1..>1631)
                     /locus_tag="PKO06_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72088.1"
                     /translation="ALCRTRDGVILAGTPEGLVRVRFEDEVFISEPVALGQAPSTNVL
                     LLKPVEDRVLIGTDRGAGVLEAATVTWYKPEPGSIITGVTAGAEMSGAVWFGGSVGLM
                     KVQGDKLVLEEREAQQKHGWVQALTGLEKSHLIGGSKGLSQRSATEDQVLMSDIWVNS
                     LYLTAGLMNIPDAVPDRRPELDQIDKVAQVLAEMGERGGWSDELREYNEMTTWFQNPA
                     NFNHPDWERRGMEMFNRLSQIPEVAMQIKPIRLPLLKGLWIGTQENGLLLWSNDGKQR
                     HFTLDNSPLPSNRITAIDGLENGETWIGTQDAGLARYRSQTVNPSQAAQTVYEGPATV
                     LKILGDRLMLGTEREGLRVYDPVSLELLARYDSSAVAGFHWRVTGVELDPKGALWVSG
                     DRGVWRFDGAKWQTFDARHGLATDVVRLLEVDSDGRVFAVCGTEGMPSEQLYQFDGTK
                     FVSYGKKTVRTILGRQGQERTTGLAFLGLQKTYLRNFDLNNASVSLGLYDQEKSSDLI
                     AGLLATPYFLLLGHANGTLSIFDGEGFKPVSENGP"
BASE COUNT          322 a          577 c          403 g          329 t
ORIGIN      
        1 cggtccgttt tccgacaccg gcttgaatcc ctccccgtcg aagatcgaca gcgtcccgtt
       61 cgcgtggccc agcagcagaa aatagggtgt cgccagcaaa cccgcgatca gatccgagga
      121 cttctcctga tcgtacaacc ccagagagac tgaagcattg ttcagatcaa agttgcgcag
      181 atacgttttc tgaagcccga gaaacgcgag gccggtggta cgttcctgcc cttgccgacc
      241 cagaatcgtg cgcaccgtct tcttcccgta actgacgaac ttcgtgccat cgaattgata
      301 caactgctcg ctcggcatgc cctcggtgcc gcacacggca aacactctgc catccgaatc
      361 gacttccagc aggcgaacga catccgtggc gaggccgtgc cgggcatcga acgtctgcca
      421 cttcgcacca tcaaatcgcc acacccctcg atcgccgctg acccacaacg cgcccttcgg
      481 gtcaagctcc accccggtca cccgccagtg gaacccggct acggcactgg aatcataccg
      541 tgccagaagt tcgaggctga ccggatcata gacgcgcagt ccttcccgtt ccgttcccag
      601 catcaggcga tcgccaagaa tcttcaggac agtcgctggc ccctcataca ccgtctgtgc
      661 cgcctggctc ggattcacgg tctgagatcg atatcgcgcc aaccccgcat cctgcgtgcc
      721 gatccaggtt tccccgttct cgagtccgtc gatcgccgtt atgcggttgc tgggcagcgg
      781 actgttgtcc agcgtgaagt gccgctgctt tccatcattc gaccacaaca gcagaccgtt
      841 ttcctgcgtt ccgatccaca gccccttcag cagaggtaac cgaatcggct tgatctgcat
      901 cgccacttcc ggaatctggc tcaggcggtt aaacatctcc atgccgcggc gttcccagtc
      961 cgggtgattg aagttggcag ggttctgaaa ccatgtggtc atttcgttgt attcccgcag
     1021 ctcgtccgac cacccgcccc gctcccccat ctcggccaga acctgcgcca ccttgtcgat
     1081 ctgatccagt tccggacgtc tatcgggaac cgcatcggga atgttcatca gtccagcggt
     1141 cagatacaag ctgttgaccc agatatcact catcaacacc tggtcttcag tagcgcttcg
     1201 ctgcgacaac cccttcgaac caccaatcag atgactcttc tcgaggccgg tcagagcctg
     1261 tacccaaccg tgtttctgct gggcttcgcg ctcctcgaga acgagtttgt ctccctgaac
     1321 tttcatcaga ccgacgctgc caccaaacca caccgcccca ctcatctcgg ccccagccgt
     1381 cactccggtt atgatcgagc ccggctccgg tttgtaccag gtcacggtcg cggcttccag
     1441 gacgcccgcg ccccggtcgg taccgatcag cacccgatcc tcgaccggct tgagcagcag
     1501 aacgttcgtc gatggcgcct gcccaagcgc aaccggttcc gaaatgaaca cctcatcctc
     1561 gaaacgcacc cgcaccagtc cttccggcgt gcccgccaga atcacaccat cgcgggtacg
     1621 gcagagagcg t
//
LOCUS       DAORHD010001237         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100845_length_1629_cov_2.995553, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001237 DAORHD010000000
VERSION     DAORHD010001237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100845_length_1629_cov_2.995553"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKO06_20450"
     CDS             complement(<1..576)
                     /locus_tag="PKO06_20450"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72089.1"
                     /translation="MKQPGQTITGGMGTRDEWKGMLLAILLLFCCGRADASLFEGLSH
                     PAWEAEKASQALEAGRLEDAEAALQRARQLQPDLAELSLNLGIVKYRKRQYDQAAREF
                     DAAARNGDQDLKAEAFYNQGNCFYRLQNWELAVRSYTNSLALREDPRTQHNLEQARKK
                     LLEQMQQQQQQQNQNGQQNQQGQQNQQGQQNS"
     gene            complement(573..>1629)
                     /locus_tag="PKO06_20455"
     CDS             complement(573..>1629)
                     /locus_tag="PKO06_20455"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV72090.1"
                     /translation="LVPEGVLMFHSPENLFLLLVAGCWLALLLRTGVRDLKRRREAFA
                     DRTILERITGHTPTRRPLLKWALLLFAFVLLCLALARPIGGLKEEAVTGMGLDLVVAL
                     DISQSMQVQDIERYARLAIAKALLQRLMQGLMHDRVGLIVFAGDTMVQCPLTLDRGTF
                     LTFLERVDSSLLTNQGTNLAGAIETSLDRFDYTASQSRAILLISDGEDHDAGRVKKAV
                     AEAKRKGVPVFTVGIGSREGGPIPVSRDVWGDVQFKVHKGRRVISRLDDEMLKMIARE
                     TGAKNWRATDGASAQEVARALMGMKRVAMVAGTRTVAQEWFELPLLIAFLLLAVEWMI
                     SERIAYTREKDHWLKRL"
BASE COUNT          333 a          510 c          434 g          352 t
ORIGIN      
        1 ggagttctgc tgtccctgct ggttctgctg cccttgctgg ttctgctggc cgttttgatt
       61 ctgttgttgc tgttgctgct gcatctgctc gagcagcttc tttcttgcct gttcgagatt
      121 gtgctgcgta cgcggatcct cgcgcaaggc cagcgagttc gtgtagcttc tcacggccag
      181 ttcccagttc tgcaggcggt agaaacaatt tccctggttg tagaaagcct cggctttcag
      241 atcctggtcg ccgtttctgg cggcagcatc gaactcgcgt gcggcctggt catactgccg
      301 ttttcgatac ttcacaatac ccagattcag tgagagttcc gcaagatcgg gctgcaactg
      361 ccgcgcacgc tgcagagccg cttccgcgtc ttccagtcgg ccggcttcca atgcctgact
      421 cgctttttca gcctcccagg caggatgcga caacccctcg aacaaggatg cgtcggctct
      481 gccgcaacag aacagcagca gaatggcaag caacatgcct ttccactcgt cacgagtgcc
      541 catgccacct gtaatcgttt gtccaggttg cttcatagac gtttcagcca atgatccttt
      601 tcgcgggtat acgcaatgcg ttcagaaatc atccattcga ccgccagcag cagaaacgcg
      661 atcagaagcg gcaattcaaa ccactcctgc gctaccgtgc gggtgcccgc gaccatcgcg
      721 acgcgtttca tgcccatcaa cgcgcgcgcg acttcctgcg cactggcacc atccgtcgcc
      781 cgccagtttt tcgctccggt ctcgcgagca atcatcttga gcatctcgtc atcgagtcga
      841 ctgattaccc gtcggccttt atgcactttg aactgcacgt ctccccagac gtcacgactg
      901 acgggaatcg gaccgccttc acgactgccg atgccgacgg tgaacaccgg gacccctttc
      961 cgtttggctt cagcaacggc tttcttcacc cttccggcgt cgtggtcttc accatcggag
     1021 atcaggagaa tggcccggct ttgtgaggcg gtgtaatcga agcgatcgag gctggtttca
     1081 atggcccccg cgaggttggt tccctgattc gtcagcagag acgaatcgac tctttccaga
     1141 aacgtcagaa aggtgccgcg atcgagcgtc agggggcact gcaccatggt atcgccggca
     1201 aagacgatca gtcctacccg atcgtgcatc aagccctgca tcaatcgttg taacagggcc
     1261 ttggcaatcg ccaggcgcgc atagcgctcg atatcctgca cctgcatcga ctgggaaatg
     1321 tcgagcgcga caaccagatc gagccccatg ccagtcacgg cctcttcttt caacccaccg
     1381 atcggtcgag ccagagccag acagagcagc acgaaggcga acagcagcag cgcccacttg
     1441 agcagggggc ggcgggtcgg ggtatgcccg gtaatgcgtt cgagaatcgt tcgatcagca
     1501 aaggcttcac gtcgccgctt cagatcacga acaccggtac gcagcagcaa tgccagccaa
     1561 cagccggcga ccagcagaag aaatagattt tcaggcgaat gaaacatcag aacaccctca
     1621 ggaaccagc
//
LOCUS       DAORHD010001238         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_100928_length_1628_cov_3.950413, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001238 DAORHD010000000
VERSION     DAORHD010001238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100928_length_1628_cov_3.950413"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PKO06_20460"
     CDS             complement(<1..265)
                     /locus_tag="PKO06_20460"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNV72091.1"
                     /translation="MINRLNDYAFKRIFGDPANKDILIDFLNTIILTDVAGPLIDLEL
                     DDREIDPEYLGDKASRLDLLATAHNGTRINIEVQIVNLGGTERR"
     gene            556..1524
                     /locus_tag="PKO06_20465"
     CDS             556..1524
                     /locus_tag="PKO06_20465"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV72092.1"
                     /translation="MSELQITWTAEAALKGERLDVALVARFAERSRAFLQNLIKEGRV
                     TVNASAVKPGYKLKAGDLVAVNFPDDTPQPSAVAATAMTLPILYEDDDLIAVNKPVGL
                     VVHPGAGREADTVVSQILGYTRLSPIGEPHRPGVVHRLDKGTSGVLVLAKTEWMHHRL
                     VKAFAGRAMTKEYLAIVRGVPEPKSGRIEVSIERDRVNRKRMTATDPDRGKPAISIYE
                     VTEIFRGAALVRVRILTGRTHQIRVHLSCIGHPLLGDSTYGGGKHEFGAGHFLHSQRL
                     SFEHPRTKQPINLEAPLPSPFESALAVLRSRPSEAQKTRKTKKRPV"
BASE COUNT          319 a          475 c          498 g          336 t
ORIGIN      
        1 tacgtcgttc cgtgcctcca aggttgacga tctgaacctc gatgttgata cgtgtgccgt
       61 tgtgcgctgt ggcgagaaga tcaagacgcg aggctttatc gccgaggtat tccgggtcga
      121 tttctcggtc atccagctca agatctatca aagggccagc gacgtcggtc agaataatcg
      181 tgttcagaaa gtcgatgagg atatccttgt tcgccgggtc gccgaagata cgcttgaacg
      241 cataatcgtt gagacgattg atcatgatgt gccgcctcta accgtttatc tctctcaggt
      301 tgttttaccg agctcatcat accattgatg ggctcacact cgctactcgc tcattcagtc
      361 cggcgctgaa ccactgaacc gaggcgagtg tcgcccatcg ctggttcaat agcgggtttg
      421 ggcttcgttg ggcgagggtg tgttcgtctg ctgtggtata ctgcgccatg gctccatgtc
      481 ttctcatcac acggcagagg caatgtggcc aggtgactga aaataacgaa tgcgtcgttt
      541 ctccgctgag tataaatgtc tgaattgcag ataacctgga cagccgaagc cgctctgaag
      601 ggcgagcggc tcgacgtcgc gctcgtggcg cgctttgccg aacgctcgcg ggcgtttctg
      661 cagaatctga tcaaagaagg ccgggtgacc gtgaacgcga gtgccgtcaa gccggggtac
      721 aaactgaaag cgggcgatct cgtggcggtg aacttccctg atgacacccc ccagccgtcg
      781 gcggtcgcgg ccacggcgat gaccctgccg attctctatg aagacgacga cctgatcgcg
      841 gtgaacaagc ccgtggggct ggttgtgcac cccggcgccg ggcgcgaggc cgacaccgtg
      901 gtgtcgcaga ttctcggcta cacgcggctc agcccgattg gcgaaccgca tcgaccgggt
      961 gtcgtgcatc ggctcgacaa gggcaccagc ggcgtactgg tgctggcgaa aaccgaatgg
     1021 atgcatcacc gactggtgaa ggcctttgcc ggtcgggcca tgaccaagga atacctcgcg
     1081 atcgtccgag gtgttcctga gccgaagtca ggtcgcatcg aggtttcgat cgaacgtgat
     1141 cgtgtcaacc gcaaacgcat gaccgcgacc gaccccgatc gcggcaaacc ggcgatcagc
     1201 atctacgaag ttaccgaaat ctttcgcgga gcggcgctgg tgcgggtgcg cattctcacc
     1261 ggccgcacgc atcagattcg cgttcacctg agctgcatcg gccaccctct gcttggcgac
     1321 tcgacctacg gtggcgggaa acacgagttc ggcgcgggcc atttcctgca cagccagcgg
     1381 ctgagtttcg agcaccctcg caccaaacag ccgataaatc ttgaagctcc cttgccgtcc
     1441 ccgtttgaga gcgcgctcgc ggtcttgcgc tcgaggccgt cagaggctca gaagactcgc
     1501 aagacgaaga agagaccagt ctgacggtgg cgggggcgag tccatcgtca cgcgccgtct
     1561 ctgctgttcc tgcggagtga cggtcacagc ccacccgggt gtgtcgcatg cgagcgtgtg
     1621 ccgcgata
//
LOCUS       DAORHD010001239         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101045_length_1627_cov_3.564885, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001239 DAORHD010000000
VERSION     DAORHD010001239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101045_length_1627_cov_3.564885"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="PKO06_20470"
     CDS             complement(<1..1290)
                     /locus_tag="PKO06_20470"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV72093.1"
                     /translation="MLEILVVDDDVKQAELLSEAVRRLGHQAAFLTSPEEALEQLEKR
                     EPDLVITDLRMPKLGGLEFLSRLKKDAPDLEVLLVTGYATVQTAIEAMRRGALDYLEK
                     PVDQRILAAKLEMVAEKVALRRENRRLRDQLERSHPEVRPLGNHPRFRAVVEQLEKAA
                     ISEASVLLQGETGTGKEVLARYLHSQSPRASGPFVAVNCSAIPETLLESEFFGHVRGA
                     FTGADRTRPGRVEEAAGGTLFLDEIGDLPLSLQPKLLRVIQNQEYCRVGDDRTRRARV
                     RWVAATHRDLAALVREGRFREDLFYRLAVLPLTIPPLRERPEDILLFLNDALERKSRQ
                     YRSAHRSFSPEALEALCSWRWPGNVRELENTVERLVLLSPSPTIDLGDLPEEFGKIVE
                     PAEPASGPTGTGLNEQVEALERRLMVQALEAARGNQSE"
     gene            complement(1296..>1627)
                     /locus_tag="PKO06_20475"
     CDS             complement(1296..>1627)
                     /locus_tag="PKO06_20475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72094.1"
                     /translation="GRLLRGLALNLLTNAVQAGAGKIEILCGFDSTHLRLELIDDGPG
                     IPDSVRRNLFMPGNTTRADGSGLGLYNAWRTAAALGGQLELAETGPGKTRFVISIPID
                     TEGEGRT"
BASE COUNT          305 a          487 c          501 g          334 t
ORIGIN      
        1 ctcagactgg ttgccgcgtg ccgcctctag tgcctggacc atgagacggc gctccagggc
       61 ttcgacctgc tcgttcaacc cggttcccgt tggtcccgaa gccggttcgg cgggttcgac
      121 aattttgccg aactcctcgg gaagatcgcc gagatcaatg gtcggtgagg gactgagaag
      181 aaccagacgt tcgacggtgt tctcgagttc gcgtacgttt cccggccacc tccaggaaca
      241 cagagcctcc agggcttccg gcgaaaaaga acggtgagcg gaccgatact gtcgagactt
      301 gcgttccagg gcatcgttca ggaacagcag tatgtcttcg ggacgctcgc gcagaggcgg
      361 aatcgtcagg ggcaggacag caaggcgata gaacagatcc tcccggaatc gcccttcccg
      421 gaccagtgcg gccagatcgc gatgtgtggc tgctacccat cgaacacggg ctcgccgggt
      481 ccgatcatct ccaacgcggc agtattcctg gttttgaatc acccgcagga gtttcggctg
      541 gagagaaagg gggagatcgc cgatttcatc gaggaacagt gtgccccccg cggcttcttc
      601 cacccgtccg gggcgcgttc gatctgctcc ggtgaaagca ccgcgaacat gcccgaagaa
      661 ttcgctttcc aacagggttt cgggaatggc gctacaattg acggcgacga atgggccgga
      721 tgcccgtgga ctctgcgagt gcagataccg ggccagaact tctttgccgg tccccgtctc
      781 tccttgcagt agaacggacg cttcgctgat ggccgccttc tccagttgtt ccacgacagc
      841 ccggaaacga ggatgatttc ccagtgggcg gacctcggga tgcgatcgct cgagctggtc
      901 gcgcaggcgg cggttttccc ggcgcagcgc gactttttcc gccaccatct cgagtttggc
      961 ggccagaatt cgttgatcta ccggcttttc gaggtagtcg agagctccgc ggcgcatggc
     1021 ttcgatcgcc gtttgaacgg ttgcatagcc ggtgaccagg agaacctcaa gatcgggggc
     1081 atcttttttc aggcgactca ggaattccag tccaccgagt ttcggcatgc gcagatcggt
     1141 gatgaccaga tccggttcgc gcttttccag ttgttcaagc gcttcttccg gtgaggtaag
     1201 aaaagcggcc tgatgaccca ggcggcgcac ggcctcggac aacagttctg cctgcttcac
     1261 gtcgtcatcg acgaccagga tttccagcat ggtcttcaag tgcgaccttc cccctccgtg
     1321 tcgatcggaa tcgagatgac gaaacgcgtt ttccctgggc cggtttcggc gagctcaagt
     1381 tgtcctccca gggctgcggc ggttcgccag gcgttgtaca ggccgagacc ggaaccatca
     1441 gcccgcgtgg tgttcccggg catgaacaga ttgcggcgaa ccgaatcggg tattccgggc
     1501 ccgtcgtcga tcagctccag tcggagatgt gtcgagtcaa acccgcagag gatctcgatc
     1561 ttcccagcgc cggcctggac ggcattcgtg agcagattca gggccagtcc gcgaagaagc
     1621 ctgccgt
//
LOCUS       DAORHD010001240         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101059_length_1627_cov_3.138041, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001240 DAORHD010000000
VERSION     DAORHD010001240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101059_length_1627_cov_3.138041"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PKO06_20480"
     CDS             <1..594
                     /locus_tag="PKO06_20480"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNV72095.1"
                     /translation="NNITGNCYRVTVAEQISIALEPLTGKLSRLGAVYKSGELTSEIA
                     LDEARKLAAQQGFELISFDARTPSDLAAAADLFKQHQVQAVFLSSDSVIAASDEIALR
                     PIVTAFPTVCALESTIRRGGLVGRVADWNELCRHGAQIGIEILEGAQPSTIPIGRTPA
                     ARTILNTETAGRLGIKVPNGFAEQASMVDQGSSRSKK"
     gene            complement(673..>1627)
                     /locus_tag="PKO06_20485"
     CDS             complement(673..>1627)
                     /locus_tag="PKO06_20485"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNV72096.1"
                     /translation="VQENVPTVRTEDGRVFMLVLDLDKAREMESKTVRIEGKAHKADD
                     VELIKVQKINVIPDHEFPAPKVEHADYQKPASILSQTADQIVIKDVRWGIQQDPATST
                     LKAKHEWETVTIKPDLVENGYFVLKPFAPKFIAAHSLFVFTFKPGGMVSKTGKESKAI
                     ALTIEAYKKVGQTYGLIKTMKKVFDIVWNLTTWENYMTLTTKFNTGDTELFVYPMTLT
                     REQTKALLVETIKQSCVNRQGEYYHTIFNNCTNNLVILANRVLPKEKRIRLWAIPNMI
                     YNFKATMPLSLAKKLKKLDLIGDPLPKVTAKNMTVDLDAAR"
BASE COUNT          325 a          456 c          467 g          379 t
ORIGIN      
        1 aacaacatta ccggaaactg ctatcgcgtc acagttgcgg aacagatctc gatcgctctc
       61 gaaccgctga caggaaagct ttcacgcctc ggagccgtgt acaaatccgg cgaactgacg
      121 tcggagatcg cgctcgacga ggcccgcaaa ctggcggctc aacaagggtt cgagttgatc
      181 agcttcgacg cccgcacccc gagcgatctg gccgccgccg ccgacctgtt caagcagcat
      241 caggtgcagg cggtgttcct gtcgtccgac agcgtcatcg ccgcgtcaga tgagatcgcc
      301 ctgcgcccga tcgtcactgc gtttcctacc gtctgcgcgc tcgaatcgac gattcgacgg
      361 ggcggtctgg tcgggcgtgt ggccgactgg aacgagctct gccgccatgg cgcgcagatc
      421 ggcatcgaga ttctcgaagg cgcgcaaccc tcgaccatac ccatcggtcg aactccggca
      481 gctcggacaa ttctgaacac cgaaaccgcc ggaaggctcg gaatcaaggt tccgaacggg
      541 ttcgcagagc aggcgagcat ggtcgatcag gggtcatcgc ggtcgaagaa atagtttcaa
      601 agtcggtgta tgcagaatcg ccccggcatc tgccggggcg attctgtttc tacctgtgtt
      661 cgaacgatcg tttcagcgag cggcgtcgag atcgacggtc atgttcttcg ccgtcacttt
      721 gggcagcgga tcgccaatca gatcgagctt cttcagcttc ttggccagcg agagcggcat
      781 cgtcgccttg aagttgtaga tcatgttcgg aatggcccag agccggatcc gcttttcctt
      841 gggtaggact cgattggcca gaatgaccag gttgttggta cagttgttga aaatggtgtg
      901 gtagtattcg ccctggcgat tcacgcatga ctgcttgatg gtctcgacca gcagcgcttt
      961 cgtctgttcg cgggtcaggg tcatcggata cacgaacagc tcggtgtcac cggtgttgaa
     1021 ctttgtcgtc agcgtcatgt agttttccca ggtcgtcagg ttccagacga tgtcgaacac
     1081 cttcttcatg gtcttgatca gaccgtaggt ctggccaacc ttcttataag cttcgatggt
     1141 gagcgcgatg gctttcgatt ccttgccggt ctttgaaacc atgccacccg gcttgaaggt
     1201 gaacacaaaa agactatgag cggcgatgaa cttgggcgca aacggcttga gtacaaagta
     1261 gccattctcg accagatcag gcttgatggt cacggtttcc cattcgtgtt tggccttcag
     1321 ggtgctcgtc gccggatcct gctgaatgcc ccagcggacg tccttgatca cgatctgatc
     1381 agccgtctgt gaaagaatcg acgcgggctt ctgatagtca gcatgttcga ccttgggagc
     1441 ggggaattca tgatcgggga tgacgttaat cttctgaacc ttgatcaact cgacatcatc
     1501 ggccttatgc gctttgcctt cgatgcgtac ggtcttgctt tccatctcac gggctttgtc
     1561 gagatcgagc accagcatga agacgcgacc gtcttcagtg cgcaccgtcg gaacgttttc
     1621 ctgaacc
//
LOCUS       DAORHD010001241         1627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101097_length_1627_cov_2.512087, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001241 DAORHD010000000
VERSION     DAORHD010001241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101097_length_1627_cov_2.512087"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1609
                     /locus_tag="PKO06_20490"
     CDS             380..1609
                     /locus_tag="PKO06_20490"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV72097.1"
                     /translation="MKHRFPSMVRALGYRNYALFFSGQGLSIIGTWMQQLAMTWLVYR
                     LTNSTTLLGVISFASMAPFFLFSPLAGVFADRHDRYRIIVLTNVLAMLQAFLAAFLTL
                     AGLITVGQIFALSAFLGTLNSFEMPARHSLVIDIVERREDLSNAIALNSTIFNLARLL
                     GPTLAGMTIALIGEAPCFFLNTLSFLPILYALHHIRPAEGDRRKSRLPIIQGIKDGWK
                     YALQSPPIRTILTELSLISLVGMPYVVLIPVVAKQVLGGEASTFGFLMGALGLGSLAG
                     SLFIASRERVRDLAQLIPSHFLLFGIGLALVALSERLVLSLPLMALTGFAMIVGWSSS
                     NTVLQSVVDDDKRGRIMSLYTMSFMGTTPLGGLAAGLLADRYGAPTTLFGCGVICACA
                     SLILGPRLRSIEMSRSE"
BASE COUNT          270 a          514 c          469 g          374 t
ORIGIN      
        1 accccgatac cgataccgac cccgatgaag acaggaaaga acttggcctc gcacggggag
       61 aggggcctgg ttttcagggt tttgggggaa ccgaacagcc ctgccccacc gtgggtgcgg
      121 agcgttcagc tttcctcccg gccggggatc agaccctctc cttgtctctt tgcctcacaa
      181 cgccatgcct gccgtcattt ccgatgcatt ccatgaaatg aagcatcagc cccggcatac
      241 ggcaagtcag ccctgcccgc cccgggggcg gggccttcga gatcggcgga tctcatccta
      301 tcatgcattg tatgcggcgg cctgcatggg cgcagaaggc ccatgcggcc cccggggaag
      361 gtatggggac atgcctggaa tgaaacaccg gtttccatcc atggtgcggg cattggggta
      421 ccggaactac gccctcttct tcagcgggca gggtttgtcc atcatcggca cctggatgca
      481 gcagctggcc atgacgtggc tcgtgtaccg cctcacgaac tccacgacgc tgctgggtgt
      541 catctcgttt gcgagcatgg cgcccttctt cctcttctcc cccctggcag gcgtgttcgc
      601 cgaccgccac gaccggtacc gcatcattgt cctgacgaac gtgctggcca tgctccaggc
      661 gtttctggcc gccttcctca ccttggccgg gctgatcacc gtcgggcaga tcttcgcact
      721 gagcgccttc ctgggcacgc tcaactcctt cgagatgcct gcccgccact ccctggtcat
      781 cgacatcgtg gagaggcggg aagacctgag caacgccatc gctctcaact cgacgatctt
      841 caacctggcg cgactcctcg gcccgacatt ggcgggaatg accattgcgc tcatcggtga
      901 agcaccgtgt ttctttctga acaccttgag tttcctgcct atcctctacg cgcttcacca
      961 tatccgtccg gctgaaggtg accgacgaaa atcacgtctt ccgattattc agggcattaa
     1021 agatggctgg aaatacgctc ttcagtcgcc cccaattcgt acgattctca cggagctgtc
     1081 gttgatcagt ctggtcggca tgccgtatgt ggttctgatt cccgtggttg cgaagcaggt
     1141 gctgggggga gaagcgtcga cgttcggctt tctgatgggc gcgctgggcc tgggatcact
     1201 tgccggctct ctgttcatcg ccagccgcga acgggtgcgc gaccttgcgc aactgattcc
     1261 gagtcacttt ttactcttcg gcatcgggct ggctctggtc gccctttccg aacgactggt
     1321 actctctctg ccgctcatgg ccctgaccgg ctttgccatg atcgttggct ggtcgtcttc
     1381 taacacggtg ctgcagtcag tcgtcgatga tgacaagcgc ggacgcatca tgagcttgta
     1441 tacgatgagc ttcatgggaa ccactccgct cggcgggttg gcggccggcc ttctggctga
     1501 tcgctatggc gcgccgacca cactgttcgg ttgcggagtc atctgcgcct gtgcgtcgct
     1561 gattctcgga ccgcgactgc gatcgattga gatgtcgcgt tcagagtaag atgacgagcc
     1621 cctcttc
//
LOCUS       DAORHD010001242         1626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101177_length_1626_cov_3.129217, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001242 DAORHD010000000
VERSION     DAORHD010001242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101177_length_1626_cov_3.129217"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PKO06_20495"
     CDS             complement(<1..765)
                     /locus_tag="PKO06_20495"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV72098.1"
                     /translation="MIDFKLTPEQELIRKSATEFAAQVIAPVAGELDEKQEFSYDVTR
                     KLAQQGFLGIIIPKEYGGAGADALTYCLVIEEVSKACAAQAVTMSLTNSLVAFPIMTY
                     GSEEVKRKYLPAMANGEKLSCFALTEPNAGSDSANQETVAVADGDSWVINGSKIFISN
                     GGVADFAVIIATTNKAAKIRGLTAFIIDKGTPGFTVGVKENKLGIRASNTAELVFNNC
                     RIPKENILGSPGRGFRIAMDTLDGGRVGVGAQASGIA"
     gene            complement(762..>1626)
                     /locus_tag="PKO06_20500"
     CDS             complement(762..>1626)
                     /locus_tag="PKO06_20500"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNV72099.1"
                     /translation="ERHAATLIAHGADVVYIVEDPQLKEYRTAPFARVLTSVIRDVQP
                     EIVLIGASITGRDLAPRVANRLRTGLTADCTGLAIEEGTGLLLQTRPAFGGNVMATII
                     CPNHRPQMSTVRPGVMKALAADPSRKGDVRKMKIGLKDIDLKVLVKEIVKETTKRVDL
                     SEAEIIVAGGRGVGSKAQFSVIEDLAAALGGEIGASRASVEAGWVGHDHQVGQTGKSV
                     SPKLYIACGISGAVQHLAGISGADFVIAVNRDAEAPIMKSADIAIVGDLHQVVPLLAA
                     EIRRVRKEASA"
BASE COUNT          312 a          528 c          451 g          335 t
ORIGIN      
        1 cgcaatgccg ctggcctgcg ccccgacgcc cacgcgaccg ccgtcgaggg tatccatggc
       61 gatacgaaac cctcgacccg ggctgccgag aatgttttct ttcggaatgc ggcaattgtt
      121 gaacaccagc tcggccgtgt tcgaagccct gatgcccagc ttgttctctt tgacgccaac
      181 cgtgaaccca ggcgtccctt tatcgatgat gaatgccgtc agcccgcgga tcttcgccgc
      241 cttgttggtg gtcgcgatga tcaccgcgaa atcagccaca ccgccgttgc tgatgaagat
      301 cttgctgccg ttgatcaccc aggagtcacc atccgcgacg gccaccgtct cctgattcgc
      361 cgaatcggac ccggcattcg gttcggtcag cgcgaaacag gacagtttct cgccattggc
      421 catggccggc aggtactttc gcttcacttc ttccgagcca taggtcatga tcggaaacgc
      481 gaccagcgag ttggtcagcg acatcgtcac ggcctgcgcc gcgcacgcct tcgagacttc
      541 ctcgatcacc agacagtagg tcaacgcatc ggctccggcg ccgccatact ccttgggaat
      601 gatgatgccg aggaagccct gctgcgccaa cttgcgcgtc acatcgtagg aaaactcctg
      661 tttttcatcg agttccccag caaccggagc gatgacctga gcggcgaact ccgttgccga
      721 tttgcgaatc agttcctgtt caggggtgag tttgaagtcg atcatgccga ggcctcctta
      781 cgaacgcggc gaatctcggc agcgagcaaa ggcaccacct gatgcagatc gcccacgatg
      841 gcaatatcag cggacttcat gatcggcgct tcggcatcgc gattaaccgc gatcacaaag
      901 tcggcgccgc tgatgcccgc cagatgctgc actgcgccac tgattccgca ggcaatgtac
      961 aacttcggac tgacggattt gcccgtctgg ccaacctgat ggtcgtgtcc aacccagccg
     1021 gcttcgaccg atgcgcgcga cgccccgatc tcgcctccca gcgctgccgc cagatcttcg
     1081 ataacgctga actgtgcttt gctgcctacc ccgcgtccac ccgcgacaat gatctcggcc
     1141 tcagacagat cgacacgctt ggtcgtctct ttcacgatct ccttgaccag caccttgaga
     1201 tcaatgtcct taagtccgat tttcattttg cgcacatcgc ctttgcgaga ggggtcagcg
     1261 gccagagctt tcatgactcc cgggcgaacc gtcgacatct gcggccggtg attgggacaa
     1321 atgatggtgg ccatgacgtt cccgccgaag gctggtcgcg tctgcagcag cagacccgtt
     1381 ccctcttcga tggccaggcc ggtacagtca gcggtcaaac cggttcgcag gcgattcgcg
     1441 actcgcggtg ccagatcgcg accggtaatc gaagccccga tcagcacgat ctcgggctgc
     1501 acatcacgaa tgaccgaagt cagcacgcgg gcgaacggag cggtgcgata ctctttgagt
     1561 tgcggatctt cgacgatgta gaccacatcg gctccgtgcg cgatcagcgt ggccgcgtgc
     1621 cgttcg
//
LOCUS       DAORHD010001243         1625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101272_length_1625_cov_3.769427, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001243 DAORHD010000000
VERSION     DAORHD010001243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101272_length_1625_cov_3.769427"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1625
                     /locus_tag="PKO06_20505"
     CDS             <1..>1625
                     /locus_tag="PKO06_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72100.1"
                     /translation="GGAPITWMDVAELPAEVSSRAVRPILLAFRYHQAPYEHQVQIQA
                     KWHRDYALLNATAHRMTVSTLLESDGNSFTSMELQLKANGKQFLEVTLPDGVDLESAG
                     LDGAPTTPTMKTKSTRLISLTGSKAVRDPSQPRSVNLVFRDKRESLKNAQRVPLSLPF
                     LDIPISSVQWTLYGPEHYVFLPLPSRMMPVPRPVRLEFFNFCLAMAPLFIGALIFGIL
                     AYKLWFFVKSVHAAATAGEAQPWTWGGVLRVVVPVLIFMAVLASVSGPMIGSITDQKV
                     HHTFGALQNRLDRNEDARQVAPSSPPSESEVYDALSEKKRDYAGKPGMVMSDEGGALA
                     TPMPKDKVGGKAQVSFRVRSARDAGALPVEIAIPTTANSITMQRDLVLALETIDMRVI
                     VLWKPLEGLLTLLLGFTGLAVFLLLAFLALSNRFIPAFLLFGFYLLSSLILEQYWSQS
                     QWPGQAIMLLALCVLTAGRLLFALRSGKAVSVAVLMLACGLVSPVWAAELDPRVEQVI
                     DVYVPFKQLEERLPKDSSLVFLKLEEYTYLRDLG"
BASE COUNT          299 a          446 c          486 g          394 t
ORIGIN      
        1 ggcggtgctc ccatcacctg gatggacgtg gcggagttgc cggccgaggt cagttcgcgg
       61 gctgtacgac ccattctcct ggcgttccgg tatcatcagg ccccgtatga acatcaggtg
      121 cagattcagg ccaagtggca tcgtgattat gcactgctga acgcgacagc gcatcgcatg
      181 accgtctcca cactgctcga aagtgacggg aactcgttca cctcaatgga gctgcagctg
      241 aaggcaaacg gcaagcagtt tctcgaagtg acacttccag atggggttga tctggaatcc
      301 gcggggctcg acggcgcgcc gacgaccccc acgatgaaga ccaagtcgac tcggctcatt
      361 tcattaacgg gttcgaaagc cgtgcgcgat cccagtcagc cacgttcggt gaacctggtt
      421 tttcgcgata agcgtgagag cctgaaaaat gcgcagcgcg tgcctctgtc gcttccgttt
      481 ctcgatattc cgatttcatc ggtgcaatgg acgctgtatg ggccggagca ctatgtgttt
      541 ctgccgttac cttcgcgcat gatgcccgtg ccgcgacctg tgcgtctgga gtttttcaat
      601 ttctgccttg ccatggcccc cctgttcatc ggagcgctga tcttcggcat cctggcgtac
      661 aagttgtggt tctttgtgaa gtccgtacat gccgcggcta cggcaggcga agctcagccg
      721 tggacctggg gcggggtgtt gcgcgtggtc gttcccgtgt tgattttcat ggctgttctg
      781 gcaagtgtct ctggcccgat gatcggttcg atcacggatc agaaagttca tcacaccttc
      841 ggcgctcttc agaatcgcct cgatcggaat gaggatgcca gacaggttgc gccatcaagt
      901 cccccgagtg aaagtgaagt gtatgacgcc ctcagcgaaa agaagcgcga ttatgccggc
      961 aaaccaggca tggtgatgag tgatgaaggt ggcgccctgg cgacaccgat gccgaaggac
     1021 aaggtcggcg gcaaagcaca ggtcagtttc cgcgttcgtt cggctcgaga cgcgggcgcg
     1081 ctgccggtgg aaatagcgat tccgaccacg gccaactcga tcaccatgca gcgcgatctt
     1141 gtgttggcgc tggagaccat cgacatgcgg gttatcgtgc tctggaaacc tctcgaaggc
     1201 ttgttgacac tgctcttagg gttcactggg ctggcagtgt tcctgctgtt ggcgttcctg
     1261 gccttgagca atcgattcat tcccgctttt ctgttgtttg gtttctacct gttgtccagt
     1321 ttgattctcg aacaatattg gtcacaatca cagtggccag ggcaggcaat catgctgttg
     1381 gctctctgtg ttctgactgc gggtcgactg ttgttcgccc tgcgcagcgg caaggctgtt
     1441 tcagtggccg ttctgatgct ggcctgcggg ttggtgtccc cggtctgggc ggctgaactc
     1501 gacccccggg ttgagcaggt gatcgatgtc tatgtgccgt tcaaacagct cgaggagcga
     1561 cttcccaaag acagcagcct ggtctttctc aaactcgagg aatacaccta cctgcgtgat
     1621 cttgg
//
LOCUS       DAORHD010001244         1624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101359_length_1624_cov_3.814532, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001244 DAORHD010000000
VERSION     DAORHD010001244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101359_length_1624_cov_3.814532"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKO06_20510"
     CDS             complement(<1..876)
                     /locus_tag="PKO06_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72101.1"
                     /translation="MSRIIAASRVACHVAFLFLLFISLPIIPLHASSFDQVWQQITRP
                     FTSDLPVFTLTLRAQADAISAPALNEPRVGDTASGPASTPPSLQASELLLSRTGSDSW
                     FLQLRSPWTNVEVRRTATETLLIVSDHLIVFSGRDLADCTNILNPTGALRRVFNQETS
                     LQGLPFLLSLGSLEALNRLPGCDKLFVPIASDVWSIDGQATLTAVLSSATAAIDIRVQ
                     PGHRKLAALSHLSLSVSTQAVVPGPIPVGLHDQPVSRVELETMILHAVRRFCSIRFPS
                     VELQQPPAVASTTGGV"
     gene            complement(993..1448)
                     /locus_tag="PKO06_20515"
     CDS             complement(993..1448)
                     /locus_tag="PKO06_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72102.1"
                     /translation="MKRLVSLLVVAALVLPLFPLTAQDAPAAPAAANLGFGIDEATFK
                     TVDAMIEKALTAYNAQDPKAFYADFAKMMAAICTDQAFETLYKTTYMTTYGKYKSRTY
                     KADASTLAEGTGLFTYEAEFEKGKALIQVNITKEENALKIMQLAFSPAQ"
BASE COUNT          352 a          407 c          534 g          331 t
ORIGIN      
        1 caccccgccg gtggttgagg ccactgccgg cggttgctgc agctcgactg acggaaagcg
       61 aatggagcaa aagcgtcgaa cggcatgaag aatcatggtt tcgagttcga cgcgtgaaac
      121 cggctgatca tgcagaccca caggtattgg ccctggtacg acggcctgtg tcgacaccga
      181 tagcgaaagg tgactcagag ccgccagctt gcgatgaccg ggctgcacgc gaatgtcgat
      241 agccgcggtc gcactgctca gcactgccgt gagcgtggcc tgtccgtcaa tcgaccagac
      301 atctgaagcg atgggaacga acagcttgtc gcacccggga aggcggttca gggcctcgag
      361 cgaaccaagg gagaggagaa acggcaggcc ttgcagggaa gtctcttggt tgaaaactct
      421 tctcaaagct cctgtcgggt tcagtatatt cgtacaatcg gccagatctc gcccggaaaa
      481 tacgatcaga tggtctgaga cgatcagaag ggtttcggtc gccgtgcggc ggacctcgac
      541 gttggtccag ggtgagcgca gttgaagaaa ccaggagtcg gagccggtgc gactgagcag
      601 gagttcagag gcttgcaggg atggcggagt ggaagcaggg cctgaagctg tatcgccgac
      661 gcgcggctca ttgagtgccg gggccgagat tgcgtctgcc tgggctcgta acgtcaaggt
      721 gaagaccggc aggtcgctgg tgaagggccg ggtgatctgc tgccagacct gatcaaacga
      781 tgaagcgtga agagggataa tcggcagaga gatgaagagc agaaacagaa aggcaacgtg
      841 acaggcgact ctgctggcgg cgataatccg actcatggct tcctctcttt ttatgggtag
      901 tttgcgatct ttcgaccagg acgatcgaga cagattgcct gggaacagac gcgggggaag
      961 gcttggcctc cccccgcgcg gtcagacgaa ggttactggg cgggcgagaa cgccagctgc
     1021 atgatcttca gggcattttc ttccttggtg atgttgacct gaatcagcgc tttgcctttt
     1081 tcgaactcgg cttcgtaggt gaagagaccg gttccttcag ccagcgtgct ggcgtcagct
     1141 ttgtaggtgc ggctcttgta cttgccgtag gtggtcatat aggtggtttt atacagggtt
     1201 tcaaaagcct gatcagtgca gatggctgcc atcatcttgg cgaagtcggc atagaaggct
     1261 ttggggtcct gggcgttata cgccgtcagg gctttctcaa tcatggcgtc aacggtcttg
     1321 aaggtcgctt catcaatgcc aaaaccaaga ttggcggcgg cgggcgcggc gggtgcatcc
     1381 tgcgccgtca gcgggaaaag agggagaacc agagcggcga caaccaacaa agagaccagg
     1441 cgtttcatga cttcctccga aagataaaat gcggggacaa gaggtacccc tcggtgatgt
     1501 acgacgagcc gccgtttttg tcaaacttcg gccggtggtc ggtacacggc gattgcctgc
     1561 gttgaccgcg cggaacggtc gtgctatgtt gcccgcatgc gtcaggccga gtttttccgt
     1621 gatt
//
LOCUS       DAORHD010001245         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101791_length_1620_cov_2.640256, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001245 DAORHD010000000
VERSION     DAORHD010001245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101791_length_1620_cov_2.640256"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PKO06_20520"
     CDS             complement(<1..393)
                     /locus_tag="PKO06_20520"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNV72103.1"
                     /translation="MKSSNQRRAITKSGNRSPIRTASTLMKTRHEPSVSAPPPLEGFL
                     RVAATVPALSLGNIEANLREHLEICRHAAAEEARLLVFPELSLTGYSLGDLLLQPEVH
                     ETAWSALLRLAHETRNLQLTIVVGLPLAL"
     gene            977..>1620
                     /locus_tag="PKO06_20525"
     CDS             977..>1620
                     /locus_tag="PKO06_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72104.1"
                     /translation="MIDLSREVLHAAIVSLIFGATFGIAEFLRLRFTTPVEVTRKFVH
                     LVGGLVSLSFGCLFASHWTLLVLCVLFVALLHVTRSHGLLASVHKVDRVSGGALYHPM
                     AIYGTFCAAQLLGRPEFYLISTMVLSVSDTIAALVGSAYGFKLYKVQEERKSFEGSIM
                     FFVSTFLIAHLGLLLLTPIGRFESVLCGILIAMCVTLFEAISLGGADNLFIPWGT"
BASE COUNT          285 a          447 c          468 g          420 t
ORIGIN      
        1 gagggcgagc ggcagaccga ccacgatcgt cagttgcaga tttctggttt cgtgcgcaag
       61 tcgcaaaagc gcactccagg ctgtttcgtg aacttctggc tgcagaagca gatcgcccag
      121 gctgtagccg gtgaggctga gctcaggaaa caccagcaac cgtgcctctt cggcggcggc
      181 atggcgacag atctcgagat gctcgcgaag attggcttcg atgttcccga gcgataaagc
      241 gggcacggta gccgccactc tcagaaaacc ctcgagcggg ggaggggctg acacgcttgg
      301 ctcgtgacgc gtcttcatca acgtcgacgc agtccgaatg ggactgcggt tcccagactt
      361 ggtgatggct cggcgttggt tgctggactt catggcttcc tcccgtgctg aatacgctga
      421 ctgcatcata accgcacccc gtcgaaaaac caacttcact gattgttttc cggtttctga
      481 agcgctatac tgcgtctggg agcagcctat gccatttgtc agacgtacac cgaagctttc
      541 atctcgcctt ttcgtctggg gtacctgggt gtttttcgcc gccatcctgt acctcttgtt
      601 ttctgtcgtc ctttctctcg ccggaaaaga gatgcctggg gtgttctcgc ccagccacga
      661 cacgatggcc gtgcaggcga cggtgccggc tccgccgccg ccaggttcgg ttctctcacg
      721 ccccgcgtcc attctcgatc agagtgttac gagaaacgac gaaggtcgtc aacaggtcca
      781 gaggctgtta caggaaagca gcgggcagta acgacccggt gtcgccaggc gttctgtccg
      841 agttgaaaca ttgataaagg ttgatcggta cggccgatgc cagcctctga atcacaaatg
      901 tatcccctga atgagacgat ccgtatgaac ggccccgtga cctcggagtt gccgaatctg
      961 aacgattgag gcggagatga tcgatctgtc gcgtgaagtt ctgcatgctg ccattgtctc
     1021 gctcatcttt ggcgcgacgt ttggcattgc cgagtttctg cggctgcgtt tcacgactcc
     1081 cgtggaagtc actcggaaat tcgtgcatct ggtgggcggg ctcgtttcgc tttcattcgg
     1141 ttgcctgttc gcctctcact ggacattact ggttctctgt gtcctgtttg tcgctcttct
     1201 gcacgtcact cgctcccatg gtcttcttgc ctcggttcac aaggtcgacc gtgtttccgg
     1261 gggggcgctc tatcacccca tggctattta tggcacgttc tgtgccgcgc agctgttggg
     1321 tcgacctgag ttctacctga tctccacaat ggtcctttcc gtctcggaca cgattgccgc
     1381 cctggtgggc agtgcctacg ggtttaaatt gtacaaggtg caggaggagc gaaagagctt
     1441 cgaaggctcg atcatgttct tcgtttccac tttcttgatc gcgcatctgg ggttgttgtt
     1501 gctgacgccg ataggcagat ttgagagtgt gttgtgtgga atactgattg cgatgtgtgt
     1561 cactctcttc gaggccatct cgcttggcgg tgcggataat ttgttcattc cgtggggtac
//
LOCUS       DAORHD010001246         1619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101905_length_1619_cov_2.323529, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001246 DAORHD010000000
VERSION     DAORHD010001246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101905_length_1619_cov_2.323529"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKO06_20530"
     CDS             <1..196
                     /locus_tag="PKO06_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNV72105.1"
                     /translation="AGGIFLAAVSLVPTILVKMMNVQFYFGGTALLIVVGVAMDTMRQ
                     LESHLVTRHYEGFMKKKGLR"
     gene            215..871
                     /locus_tag="PKO06_20535"
     CDS             215..871
                     /locus_tag="PKO06_20535"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNV72106.1"
                     /translation="MNLILLGPPGAGKGTQAKMLVDRYHIPQVSTGDLLRAEIANQSD
                     LGKKAQTYVQSGGLVPDELVMSMLLQRIAKPDCQQGFILDGFPRTVKQAENLEKSLSD
                     KGEKIAGVLCVQVPDEPLLERLTGRRICRGCSASFHVTFSPPKKDAVCDHCQGELYQR
                     KDDSREVIANRLKVYHEQTSPLIQFYQGRKLLHAIDGLGKIEMIFSKMCDILDVLKSH
                     "
     gene            868..>1619
                     /gene="map"
                     /locus_tag="PKO06_20540"
     CDS             868..>1619
                     /gene="map"
                     /locus_tag="PKO06_20540"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNV72107.1"
                     /translation="MIPLKTKTELDIMRENGLLLSGILDGVKRMIRPGVTTLELDQYL
                     ESEIRKGGAVPAFKGYRGYRASLCASVNEEVVHGIPSSRRRLKDGDIISLDVGLKKQG
                     LFSDMAVTVPVGKVSRDVQSFLRISEESLWEAIKNVKPGNRVGDLSHAVQAYVERHGY
                     SVVRDYVGHGVGRQLHEEPVIPNFGPPHVGPRLEVGMVLAIEPMVNVGGYEVEVLDDG
                     WTVVTADGSLSAHFEHSVAVTEDGPLVLTLPV"
BASE COUNT          349 a          409 c          501 g          360 t
ORIGIN      
        1 cgccggcggt atctttctcg cggcggtttc tctggtgccg acgatcctcg tgaagatgat
       61 gaatgtccag ttctacttcg gtggaacggc gcttctgatc gtcgtcggcg tggccatgga
      121 taccatgcgg cagctcgaaa gccacctggt gacccgccat tacgaaggct tcatgaagaa
      181 gaagggcctg cgctaaacca tgggtggaac gaaactgaat ttgatccttc tcggcccccc
      241 gggggccggg aagggtaccc aggccaaaat gctggttgat cggtatcata ttccccaggt
      301 ttcgaccggt gatcttctgc gcgcggaaat cgccaatcag agtgatctgg gcaagaaggc
      361 tcagacgtat gtgcagtccg gtggactggt tcccgacgaa cttgtcatgt cgatgctgct
      421 gcaacgtatt gccaagcctg attgtcaaca gggattcatt ctcgatgggt ttccacgcac
      481 ggtgaagcag gccgagaatc tggaaaaatc gctcagcgac aaaggggaga aaatcgccgg
      541 agtgctgtgc gtgcaggttc ccgatgaacc gttactcgag cgtctgaccg gccgccgcat
      601 ctgccgcggc tgcagcgcca gttttcatgt caccttttcg ccgccgaaga aagacgcggt
      661 ctgcgaccac tgtcagggtg aactgtatca acgcaaggac gacagccgcg aggtcatcgc
      721 gaaccggctg aaggtctatc atgaacagac cagtccgttg attcagtttt atcagggtcg
      781 caagctgctg catgccatcg acggtcttgg aaaaatcgag atgatttttt ccaagatgtg
      841 tgatatacta gacgttctaa aatcgcattg atcccgttga aaaccaaaac tgagcttgac
      901 atcatgcggg agaacggcct tctcctcagt ggaatccttg atggcgtcaa acgcatgatt
      961 cgtccgggag tcacgactct cgagctggac cagtatctcg agtcagagat tcgcaagggc
     1021 ggcgccgttc cggcgttcaa ggggtatcgc gggtatcgcg ccagtctgtg cgcgagtgtg
     1081 aatgaagagg tggtgcatgg catcccatct tccaggcgtc gcctgaaaga tggagacatc
     1141 atcagtctcg atgttggttt gaaaaaacaa ggtttgtttt cggacatggc ggtcacagtg
     1201 ccggtcggga aggtgtcgcg cgacgttcag agtttcctcc ggatctcgga agagtccctg
     1261 tgggaagcga tcaagaacgt gaagcccgga aatcgggtag gcgatctttc tcatgcggtg
     1321 caggcctacg ttgaacgcca tgggtactcc gtagtgcgtg attacgtcgg ccacggcgtc
     1381 ggaaggcagt tgcacgaaga gccggtcatt ccgaacttcg gtcctccaca tgtagggccg
     1441 cggctggaag tcggaatggt tctcgcgatc gagcccatgg tcaacgtggg tggctacgaa
     1501 gtggaagtgc tggatgacgg gtggacggtg gtaacggccg atggtagttt atcggcgcat
     1561 ttcgaacatt cggtggcggt gacagaagac gggcctctgg tcctgactct gccggtcga
//
LOCUS       DAORHD010001247         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101966_length_1618_cov_3.319258, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001247 DAORHD010000000
VERSION     DAORHD010001247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101966_length_1618_cov_3.319258"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKO06_20545"
     CDS             complement(<1..345)
                     /locus_tag="PKO06_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72108.1"
                     /translation="MTGDPNEFSLLHPDSVLMLTPSDVLEYLFCPRFPYFMHCLGISQ
                     YEERRYKIRVGRELHRRRARQNPTYKRKRLGCKKRVTDVYLACPTLHMRGIVDEVLEL
                     EDGSLAPLDYKHT"
     gene            complement(342..740)
                     /locus_tag="PKO06_20550"
     CDS             complement(342..740)
                     /locus_tag="PKO06_20550"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNV72109.1"
                     /translation="MTDRETLLAYRWQQAAETLADARSMLRAGVSPRSIINRAYYAAF
                     YGVLALFLSERVTVKSGKHVGIIGAFNEVFIRTGIIPKEYSRTLQRLFERRQRGDYME
                     LEQLTEDDAAQQVALSESFVAMVRNKLGIS"
     gene            complement(737..1057)
                     /locus_tag="PKO06_20555"
     CDS             complement(737..1057)
                     /locus_tag="PKO06_20555"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV72110.1"
                     /translation="MLCEQDRGLAQTLKARLRQHIPILDFRVFGSRARGNASSDSDLD
                     VFIVIPERNQAHETAISDISWEVGFDHRVIILPLVVSQVELRETALRSAPIVENIMRE
                     GISV"
     gene            complement(1109..>1618)
                     /locus_tag="PKO06_20560"
     CDS             complement(1109..>1618)
                     /locus_tag="PKO06_20560"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HNV72111.1"
                     /translation="RWQGKQGVIMGFHAGAELLVRLPLLGRSLQLERLETTVTDVEMQ
                     FRRQTVELCASPNVYRFQSPWLPFNQENYAKYQGMSIDEKRLECERIATGNLLTAFRG
                     LGVHISDRLSVQFEKQRQVRCRYKNQVFIGFYGALRVNARLPDGIAIGKAVSHGFGWL
                     SPAPDHRQS"
BASE COUNT          379 a          440 c          437 g          362 t
ORIGIN      
        1 cgtatgcttg taatccagag gggccagcga gccgtcctcc agctcgagca cctcgtcaac
       61 aattccacgc atgtgcaaag tcgggcatgc gagatacacg tcggttactc gcttcttaca
      121 ccctagtcgc tttcgtttgt acgttgggtt ctggcgtgct cttcgtcggt gcagttctcg
      181 acccacgcgg atcttgtagc gccgctcctc atactgggaa atgccgaggc agtgcatgaa
      241 atagggaaac cgcgggcaga acagatactc gagaacatcg ctcggggtca gcatcaacac
      301 cgagtctgga tgcaaaaggg aaaactcgtt cgggtccccg gtcatgagat cccgagcttg
      361 ttccggacca tagcgacgaa agattcgctg agagccactt gttgtgcggc gtcatcttcc
      421 gtcagctgtt cgagttccat gtaatcgcct cgctgtcggc gctcaaagag cctctgcaga
      481 gtgcgtgagt attcctttgg aatgattcct gtccggataa atacttcgtt gaatgcgcca
      541 atgattccca cgtgtttgcc tgacttcacc gtaacgcgct cagagagaaa gagagcaaga
      601 acgccgtaaa aagccgcgta gtaagcccga ttgatgatgg accggggtga tactccggcg
      661 cggagcatgc ttcttgcgtc cgcaagcgtt tctgcggcct gctgccagcg gtaagccagc
      721 aatgtctcac gatcggtcac acgctgatcc cctcgcgcat gatgttttca acaatcgggg
      781 cagatctcaa ggcggtttct ctcaattcca cctgcgaaac aacaagcgga agaatgatga
      841 cgcgatggtc gaagccgact tcccacgaaa tgtcggaaat ggcggtttca tgggcttggt
      901 ttcgttccgg aatcacgata aacacatcaa gatctgaatc cgatgaggca ttgccacggg
      961 cccgtgagcc gaagacgcga aaatccagaa ttggaatgtg ttgtctcagt cttgccttga
     1021 gggtctgggc aagccctcga tcctgctcgc aaagcatgag ttgttgactc ctgggcgaaa
     1081 agcgttcaga ggtctttctt tcattgtatc acgattgccg gtggtcgggt gccggagaga
     1141 gccaaccgaa accatgactg acagccttcc cgatggcgat accgtcaggc aggcgggcgt
     1201 tcacccggag agcaccgtag aaaccgataa agacttgatt cttgtaccga cagcgcacct
     1261 gcctctgttt ctcgaactga accgacagtc gatctgaaat gtgaaccccc agtccacgaa
     1321 aagcggtaag caagttgccg gtggcgattc tctcgcattc caggcgcttc tcatcaatac
     1381 tcataccttg atacttcgca tagttttcct gattgaacgg aagccagggc gactggaacc
     1441 gataaacatt gggcgatgcg cacaactcaa ccgtctgtcg acgaaattgc atttcgacat
     1501 cggtgacggt ggtctcaaga cgttcaagtt gcagagagcg acccaggaga ggaagacgaa
     1561 ccaggagttc cgcgcctgca tggaaaccca tgataacacc ttgcttccct tgccatcg
//
LOCUS       DAORHD010001248         1618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_101969_length_1618_cov_3.228407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001248 DAORHD010000000
VERSION     DAORHD010001248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101969_length_1618_cov_3.228407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1618)
                     /locus_tag="PKO06_20565"
     CDS             complement(<1..>1618)
                     /locus_tag="PKO06_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72112.1"
                     /translation="LLNIASQESQSLDSDARRILLAEQNDLLQALEPAEFLQSVHDEL
                     DREDGFWNDREGSVRQAEKGVDPRFVTEAALPRRLKHLRERYALQPLYLYFADTDIRT
                     IWWQAFSSLAEDYSANDLEWFGRGHAQFSAAPSRAPTWLPDSRPLMPADRAINERGLV
                     PNKVYEQLAERMFGVYWNRIRLPHDIVQGYSERFSSTIFLCSRFLRSSASYHASTFGG
                     YGAVFSLAQLPRTTILLRALSSTRQRAVAHRSLHFVLPGTPMGFFRCGDHLALQTLLP
                     TEFHHGLPSPAPGLQPALVVATDHAARRNIWRHAVLLFDVCVLACLLVTTASGWYWCR
                     QTTRLSVSSRWKLTAIVTCAGIIPVAVLGFLLLRLLWLSERDYSRRVLALTQSRVRSL
                     ASTLEEQLKKGALRLQQLKREVESFSRGDANEQQPAFSQAFWLKDLMKLAFLYRRDGS
                     SLFWPDRDADHQLHEVLQPTSRLFFDELGVIQKTERERTRDGALADTFDFFWGVNRAD
                     LAFAQEGLETRSVVSFQEDQRMQFWILASDT"
BASE COUNT          370 a          442 c          511 g          295 t
ORIGIN      
        1 gagtgtcgga tgcgaggatc cagaactgca tgcgctggtc ttcctgaaac gagacgactg
       61 atcgtgtttc aaggccttcc tgggcaaacg ccagatcggc gcggttaaca ccccagaaaa
      121 agtcgaacgt atctgccaga gcgccatctc tcgtgcgttc gcgctcggtc ttttgtatga
      181 ctccgagctc gtcgaaaaag agacgactgg ttggctggag cacttcatgt aactggtggt
      241 ctgcatctcg atcaggccaa aacagggaag agccgtcgcg tcggtacaag aaggccagtt
      301 tcatcagatc tttcaaccag aaagcctgcg aaaaggccgg ttgctgctcg ttggcgtccc
      361 cgcgggagaa ggactcaacc tcacgcttca gctgctgcag ccgcagcgcg cctttcttca
      421 attgctcttc caacgttgag gcgagtgagc gcacgcgact ttgcgtcagc gcgagcacac
      481 gtcgcgaata atctcgttcg ctcaaccaca acaggcgaag aaggagaaag ccgagcaccg
      541 ctacgggaat gatgccggcg cacgtgacaa tcgcggtaag cttccagcgt gaagaaaccg
      601 acaggcgggt cgtctgtctg caccagtacc agccactcgc ggttgtcacc agcaaacagg
      661 cgagcacaca gacatcgaac agcaaaacgg cgtggcgcca gatgtttcgt ctggcggcgt
      721 gatcggtggc gacgacgagc gccggttgca gaccgggtgc gggtgagggc agaccatggt
      781 gaaactccgt gggcagcagg gtctgcagag cgagatggtc gccgcagcgg aaaaaaccca
      841 tcggggttcc tggaagcaca aagtgcagcg agcggtgggc gacggctctc tgtcgggtcg
      901 agctcagcgc gcgcaggaga atggtcgttc gaggcagttg cgccagcgag aatacggctc
      961 catagccgcc gaaggtactg gcgtgatagg acgcggatga tctgagaaag cgtgagcaga
     1021 gaaagatcgt ggagctgaac ctctcggaat acccctgcac gatgtcgtgt ggaagccgga
     1081 ttcgattcca gtacacaccg aacatgcgtt ccgcgagttg ttcgtatacc ttgttgggca
     1141 ccaagcctcg ttcattgatc gcacgatcag cgggcatcag ggggcgggag tcgggaagcc
     1201 aggttggcgc acgactcgga gccgcgctga actgcgcgtg tccgcggccg aaccactcga
     1261 gatcattggc cgaataatct tcggcaagag aggaaaaggc ctgccaccag atggtacgga
     1321 tgtcagtatc ggcaaagtag agatagagag gttgcagcgc ataccgttcg cgcaagtgct
     1381 tgagccgtcg gggcaatgcg gcctcggtca caaagcgggg gtcgacaccc ttttccgcct
     1441 gacgaaccga gccttcgcga tcgttccaga acccgtcttc gcggtcgagc tcatcatgga
     1501 ccgactgcag aaactcagcg ggttcgagtg cctgcaacag gtcgttctgt tccgcgagta
     1561 agatgcgacg ggcatcggaa tcaagcgatt gtgactcctg agaagcgatg ttcaacag
//
LOCUS       DAORHD010001249         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102170_length_1616_cov_3.385010, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001249 DAORHD010000000
VERSION     DAORHD010001249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102170_length_1616_cov_3.385010"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PKO06_20570"
     CDS             complement(<1..479)
                     /locus_tag="PKO06_20570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNV72113.1"
                     /translation="MAKYRIAWLPGDGVGVDVMDAARKVLDFLKVDAEYIHGDIGWEF
                     WCKEGNPLPDRTIDVLKKTDVALFGAITSKNKEDAVKELAPELQGKGLVYRSPIVVMR
                     QMFDLYSCVRPCKAFKKNPLNFKENIDITVFRENTEGLYAGVEFCPIPEDVRAAIEK"
     gene            complement(525..1019)
                     /locus_tag="PKO06_20575"
     CDS             complement(525..1019)
                     /locus_tag="PKO06_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNV72114.1"
                     /translation="MKFTGKVFKYGDDVNTDVIFPGRYLNVFEPAEMAKHAMEDLDKD
                     FVKNVKPGDIVVAGKYFGCGSSREQAATCLKAAGVSVVIAESFARIFYRNAINLGLAI
                     MVCPGIGQIVENGDQLEVEPTSGAVKNLTRKGETKAKPLPPFILGILDDGGLIPHLQK
                     SLKK"
     gene            complement(1016..>1616)
                     /locus_tag="PKO06_20580"
     CDS             complement(1016..>1616)
                     /locus_tag="PKO06_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008441644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HNV72115.1"
                     /translation="GAKAAMIAPDQKTIDFVKSVTDTPFEPIFPDPHAVYARTIEIDA
                     AKLTPQVACPHMVDNVKPVSEIAGLPIHQALLGSCTNGRLEDLEIGAQLLKGRKVSPD
                     VRLIVIPASWQVYREAMDKGYLRTYIDAGAVIVNPGCGPCLGAHQGLLAPGERCISST
                     NRNFKGRMGSTEAEVYLASPATVVASAIAGQITRPEVKA"
BASE COUNT          304 a          468 c          488 g          356 t
ORIGIN      
        1 ttcttctcga tcgcggcacg cacgtcttcg ggaatcgggc agaattcgac cccggcgtac
       61 aaaccttcgg tattttcccg aaacacggtg atgtcgatgt tctctttgaa gttgagcggg
      121 ttcttcttga acgccttgca cgggcgcacg caggagtaca agtcgaacat ctggcgcatg
      181 acgacgatgg gcgagcgata gaccagacct ttgccctgca gctcgggggc cagttctttc
      241 accgcgtctt ccttgttctt cgaggtgatt gcaccgaaca gtgcgacgtc ggtcttcttg
      301 agcacgtcga tggtgcggtc aggcagcggg ttgccttcct tgcaccagaa ttcccagccg
      361 atatcgccgt gaatgtattc cgcatcgact ttcagaaaat cgagaacctt gcgagcggca
      421 tccatgacgt cgacgccgac gccgtctccg ggcaaccagg caatacgata cttggccatg
      481 atgtcagaat tctccttccc ttttcaggta aatgtgtacg agttttactt tttcagactc
      541 ttctgcagat gcggaatcaa gccaccgtca tccagaatac caagaatgaa gggcggcagc
      601 ggcttggctt tggtttcgcc cttgcgggtg aggttcttga ccgctccgct ggtgggctcg
      661 acctcgagct ggtcgccatt ctcgacgatc tgaccgatgc cggggcagac catgatcgcc
      721 agacccaggt tgatggcatt gcgatagaag atgcgggcga agctctcggc aatgaccact
      781 gatacgccgg cggctttcag acaggtggca gcctgttcgc gcgaactgcc gcagccgaag
      841 tatttgccgg ccacgacgat gtcgccgggt ttcacgttct tgacgaaatc cttgtcgagg
      901 tcttccatgg cgtgcttggc catttcggcc ggttcgaaca cgttcaggta gcgaccgggg
      961 aagatgacat cggtgttgac gtcgtcgccg tacttgaaga cttttccggt gaatttcatg
     1021 ctttcacctc cgggcgggtg atctgacccg cgatcgccga agcgaccacg gtcgcggggg
     1081 aagccagata gacctcagct tcggtgctgc ccatgcgacc cttgaagttg cgattggtcg
     1141 agctgataca gcgctcgccg ggagccagca gcccctgatg tgcgccgaga cacggaccgc
     1201 aaccgggatt cacgatgacc gcgccggcat cgatgtaggt gcgcaggtag cctttgtcca
     1261 tcgcttcgcg atagacctgc cacgaggccg ggatgacgat cagacggaca tcggggctga
     1321 ctttgcgccc cttgagcagc tgtgcgccaa tctcgaggtc ttcgagacgg ccgttggtgc
     1381 acgaacccag cagagcctga tgaatcggca gaccggcaat ctcgctgacc ggcttgacgt
     1441 tgtcgaccat atgcgggcag gccacctgcg gcgtcaactt cgccgcgtcg atctcgatcg
     1501 tgcgggcata cacggcatgc gggtccggga agatcggctc gaacggcgtg tcggtcaccg
     1561 acttcacgaa gtcgatggtc ttctggtcgg gggcgatcat ggcggctttg gcaccc
//
LOCUS       DAORHD010001250         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102183_length_1616_cov_3.071108, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001250 DAORHD010000000
VERSION     DAORHD010001250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102183_length_1616_cov_3.071108"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1432
                     /locus_tag="PKO06_20585"
     CDS             149..1432
                     /locus_tag="PKO06_20585"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNV72116.1"
                     /translation="MKMRSLTLWCRLLCLGAVLCAGMLAAMTPPPEPQAAPAAPGVST
                     AVKDIGNGCNDFALKMLTELSKSAATENVFFSPFSVHVALAMTFAGAGSDTLQEMQKV
                     LGYPLESGSLAQTYKAYLESLRPPTGAEAPYELAVANAIWAQGKYPFGASYLKKIEDW
                     FASKIHLVDFAANANAIRVEINTWVEKITHDRIKDLIPSGMLSADTRMVLVNAVYFKG
                     KWQDEFLKSDTRDEPFTTGDGSPKTVPMMSRTANMLYAETEQYQALRLPYKGKDLAML
                     VFLPREKNGIDAMIAGLDADKIAETGRQFTGREVQLFLPKFTTDYGCTLNDPLQQLGM
                     KAAFTESADFSGMFDGGKSPTGAFYISAIVHKAFVAVDEEGTEAAAATAVAMTQECVM
                     ERVPPVVFRADHPFLFAIMHLPSETMLFVGRVMKP"
BASE COUNT          332 a          416 c          503 g          365 t
ORIGIN      
        1 cggggtgttg aaccactggt cggctgattc gctgtatctt ccgttcgctc gtgctcgggc
       61 accgtgagag agttctgacc cgctggactg tgttacactg cgcttgaaga ggatgcctga
      121 tctgttgcga aatcatgtgg aggatgtcat gaaaatgcga agcctgaccc tgtggtgtcg
      181 tctgctttgt cttggtgccg ttttgtgtgc cggtatgctt gccgcgatga cgccgccgcc
      241 cgaacctcag gccgctcccg cggctcccgg agtgtcgacg gcggtgaaag acatcggaaa
      301 cgggtgtaac gattttgcgc tgaagatgct gacggaattg tccaagtcgg ctgcgacgga
      361 aaacgtgttc ttctctccgt tcagcgttca tgtcgctctg gcgatgacct ttgccggggc
      421 gggcagtgac acgcttcagg aaatgcagaa agttctgggc tatccgctcg aatcaggttc
      481 tctggcgcag acctacaaag catatctcga atctcttcgc ccgccgaccg gggctgaagc
      541 gccgtatgaa ctggccgttg ccaacgccat ctgggcccag ggaaaatacc cgtttggtgc
      601 gtcctacctg aaaaagattg aagactggtt tgcatcaaag attcatctgg tcgattttgc
      661 agccaatgcc aatgcgattc gcgttgagat caatacctgg gttgagaaga tcacccatga
      721 ccgcatcaag gatctgattc cgtccgggat gctcagcgcg gacacgcgca tggtgctggt
      781 caatgcggtg tatttcaaag gcaagtggca ggatgagttt ctgaagtcgg acactcggga
      841 tgaaccgttc acgaccggtg atggttcacc caaaacagtt ccgatgatgt cgcgaacggc
      901 gaacatgctc tatgctgaaa ccgagcagta tcaggcgctt cgacttccct acaagggaaa
      961 ggacctggcg atgctggtgt tcctgccgcg tgagaagaat ggcatcgatg ccatgattgc
     1021 gggtctcgat gcggacaaga tcgccgaaac cggacgacaa ttcaccgggc gtgaagtgca
     1081 gttgttcctg ccgaagttca ccacggacta tggctgcacg ttgaacgatc cgctgcagca
     1141 gctgggtatg aaggctgcct ttacggaaag tgcggacttc agcggcatgt ttgatggcgg
     1201 caagagcccg acgggagctt tttatatctc agctatcgtc cataaagcct ttgtcgcggt
     1261 cgatgaagag ggaacagagg ccgcagcggc gacggcagtg gcgatgaccc aggagtgtgt
     1321 catggagcgt gtgccgccgg tcgtgtttcg ggctgaccac ccgttcttgt tcgccatcat
     1381 gcacctgcca agcgagacca tgctgttcgt cggtcgggtg atgaagccat agggctcgaa
     1441 cgcatcatcg ccgatgggtc gtttccgcag gggaatgtgc gagttgacgc gtcaagcgat
     1501 cttgttacgg cgctgatgtc ggactcgtcg aaagaaaaac cggacccgtg gcggaccagg
     1561 tgtgcggtgg ggtggagaag cgttcctcag cgtcctttac cggctggaaa tcctgg
//
LOCUS       DAORHD010001251         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102301_length_1615_cov_2.907692, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001251 DAORHD010000000
VERSION     DAORHD010001251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102301_length_1615_cov_2.907692"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1615
                     /locus_tag="PKO06_20590"
     CDS             <1..>1615
                     /locus_tag="PKO06_20590"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNV72117.1"
                     /translation="TSLSLGCLKSYWQRRSGRPNVEVLDWDMNLGNESLINRLILARP
                     AVLGFATYIWSLAPVVAVAGAVKAALPETRIVLGGPEVLFEGETLMNQYPWIDVIVRG
                     EGEQTWEELLERHLSGSSLAGCAGVLWRGDSGVVRETERGYLQNLDDIPSPFRMGLYG
                     TGRGFTYYEASRGCPFRCAYCLSSVLGPVRHFSLARVIDDLSWFMESEFTQVRFADRT
                     FNFELERAETIVRHILDHNRRQVSFHFELKADLLSDGLIDLLGQAPVDGFHLEIGVQT
                     THKPALDAVYRRSDLEVLAEKIVRLRERTRAHVHLDLLAGLPEETFPLFQKTLNDAFS
                     WNPSTIQVGLVKVLKGTALQQRVASGDLVCAPLPLYPVVRSRWLSPEEVVLIQDMGKL
                     VEGIHNPGRFSHTLSFLISQAFAEDAARFYEHLARYYRRTGRLFYQLGPEAVGQSLTA
                     FTEEFPEAKRLKPVVEALTLHELRLTQKVPAGKLGPSPLGAQAIGRQKHGWLRFKPGL
                     RAFWYDVDLQPLIGQQASKSVLCEALPSPI"
BASE COUNT          290 a          459 c          494 g          372 t
ORIGIN      
        1 caccagcctg tcgcttggct gtctcaaatc ctattggcag cgccgttctg gccgtcccaa
       61 cgtcgaagtg ctcgactggg acatgaacct cggaaatgag tctctgatca accgtttgat
      121 cctggcacgg ccggccgtgc tcggctttgc gacttacatc tggagtctgg cgccggtcgt
      181 cgcggttgcg ggggcggtga aagccgcact gcccgagacg cggatcgttc tcggcggacc
      241 cgaagtgctc ttcgaggggg agaccctcat gaatcagtac ccctggatcg acgtcatcgt
      301 ccggggagaa ggcgaacaaa cctgggaaga acttcttgaa cggcatctct ccgggtcttc
      361 gctcgcgggg tgtgccgggg tcctgtggcg cggtgacagt ggcgtggttc gggaaactga
      421 acgtgggtat ctgcagaatc tcgatgacat cccatctccg tttcgaatgg ggttgtacgg
      481 aaccggacgc gggttcactt actacgaggc ttcgcgcggg tgcccgtttc gctgtgccta
      541 ctgcctgagc tcggttctcg gtccggtgcg tcacttctct ctggcacggg tgatcgacga
      601 tctctcctgg ttcatggaat cagagttcac gcaggttcgg tttgccgatc gcacgttcaa
      661 tttcgaactg gaacgggccg agaccatcgt gcggcacatt ctcgatcaca atcgtcgcca
      721 ggtctcgttt cattttgagc tgaaggcgga tctactctcc gatggcctga tcgacctgct
      781 gggccaagcg ccggtcgatg gattccatct cgagatcggc gtgcagacga cccacaaacc
      841 ggctcttgat gccgtgtatc gtcgatcaga tctggaggtc ctggcggaaa aaattgttcg
      901 tttgcgtgag cgaaccagag cacatgtgca tcttgacctg ctggcggggc tgcccgaaga
      961 aacctttccc ctgtttcaga aaacgctgaa cgatgccttt tcctggaacc cgtcgacgat
     1021 tcaggttggt ctggtcaaag tcttgaaagg aacggcgtta cagcagcggg tggcctctgg
     1081 cgatctggtc tgcgcgcctc tgcctctgta tccggttgtc cgatcgcgct ggctttctcc
     1141 tgaagaggtg gtgctgattc aggacatggg caaactcgta gaaggtattc acaacccagg
     1201 tcgttttagc cacacgctgt catttctgat ttcacaggct tttgcggaag atgcggcgcg
     1261 gttttacgag catctggccc ggtactatcg tcgcaccggt cgtttgttct atcagcttgg
     1321 tccggaagcg gtcggccagt ctctgaccgc tttcaccgag gagttcccgg aggcaaagcg
     1381 gttgaaaccc gtggtcgaag ccctgaccct gcatgaactg cggctcaccc agaaagtgcc
     1441 cgcaggcaag ctgggcccgt cgccgctggg agcacaggcg atcggtcggc agaaacatgg
     1501 ctggttgcgg ttcaagccgg gcctgcgggc attctggtac gacgtcgacc tgcagcccct
     1561 gatcggtcaa caggcgtcga aatcagttct gtgtgaagcg ttgcccagcc cgatc
//
LOCUS       DAORHD010001252         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102584_length_1612_cov_3.998715, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001252 DAORHD010000000
VERSION     DAORHD010001252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102584_length_1612_cov_3.998715"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PKO06_20595"
     CDS             <1..371
                     /locus_tag="PKO06_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72118.1"
                     /translation="SVSTTGLETSVPVNIVHYVFDPEARTLTRKVRTHPSLATSGSDT
                     ESQVIGQNIASFSICPREIFSVRFFDIELSCMPRNPKANDTRIVLRSAVCSDFETRLK
                     RHPYHSPNRRSRLSFPPEAP"
     gene            complement(426..>1612)
                     /locus_tag="PKO06_20600"
     CDS             complement(426..>1612)
                     /locus_tag="PKO06_20600"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HNV72119.1"
                     /translation="KMLVTTLDYNDYVGRIAIGRVFSGELKNKMDVYRVRPNNPTVRG
                     KIAHLYTFQGVKRTEVDSAYAGDIVALSGVEGVSIGDTLSEIESEPLYVIPIDQPVIS
                     MMFAPNTSPFSGRDGKFLTARQIQARLLKELETNVAMRVEPTDRPDTFMVSGRGELHL
                     GILIETMRREGYEMQVSKPAAILHTDKDGNVTEPIEELTVDIPESYMGAVMEKLGPRR
                     GLLQSSKKLPDNYIRLIFTVPARGLLGFRSLFLTLTNGYGTMNQAFFDYEPYRGDIPQ
                     ERNGVMISMESGWSTAYAIKNLAERGVLFYAPMEEIYEGLVVGERPYPVDMYVNITKK
                     KHLTNVRSSTSEELERLTPPRRMSLDQALEYVADDEWVEVTPKACRIRKREINFKKRT
                     GG"
BASE COUNT          336 a          474 c          437 g          365 t
ORIGIN      
        1 acagcgtctc gacaaccggt ctcgaaacat ccgttcccgt gaatatcgtt cactacgtat
       61 ttgatccgga agcacgcacg ctcaccagaa aggttcgcac gcatccaagc ctggctacgt
      121 ctggatccga taccgaatcg caggtcatcg gtcagaacat cgcctcattt tcgatctgcc
      181 cgcgcgagat tttctcggtg cgttttttcg acatcgaact gagctgcatg ccacggaacc
      241 cgaaggccaa cgatacacgc atcgtcttgc gatcagcggt ttgctccgat ttcgagacga
      301 gactgaagcg acatccgtac cattcgccaa accgtcgatc gcgcctcagt tttccgccag
      361 aggctccgtg aacccgagag ttcggaagaa ttcgaacctg aaccgaaccg tgaggattct
      421 ggcgatcagc cgcccgtgcg ctttttgaag ttgatctcgc gtttgcggat acgacaggct
      481 ttgggtgtca cctcgaccca ctcgtcatcg gcaacatact ccagtgcctg gtcaagggac
      541 atgcgccgcg gtggagtgag tcgctcaagt tcttcgctgg tcgaggagcg cacattggtg
      601 aggtgcttct tcttggtgat gttcacatac atgtcaacgg ggtaggggcg ctcgcccacc
      661 acgagacctt cgtagatctc ttccatcggc gcataaaaca gaacgccgcg ttcggccagg
      721 ttcttgatcg cgtacgccgt cgaccagcct gattccatcg agatcatcac gccattgcgt
      781 tcctgcggaa tatccccacg atatggctcg tagtcaaaaa acgcctggtt catggttccg
      841 tacccgttgg tcagggtgag aaacagcgat ctgaagccga gcaagccccg ggccggcacg
      901 gtgaaaatca agcgaatgta gttgtcgggc agcttctttg acgactgcag cagcccacgc
      961 cgggggccga gtttttccat gaccgcgccc atatacgact cggggatatc gacggtcagt
     1021 tcttcgatgg gctctgttac gttgccatct ttatcggtgt gcagaatcgc cgcgggtttg
     1081 ctgacctgca tctcgtagcc ttcacggcgc atcgtttcaa tcagaatgcc cagatgtagt
     1141 tcgccgcgac cgctgaccat gaaggtgtcc gggcgatcag ttggctcgac acgcatcgcc
     1201 acgttggttt ccagttcttt caacagacgg gcctgaatct gtcgtgcggt caggaactta
     1261 ccatctcggc ccgaaaacgg gctggtgttc ggtgcaaaca tcatcgagat gaccggctga
     1321 tcgatgggaa tgacatacag cggctcagat tcgatctctg acagagtgtc gccgatcgag
     1381 acgccctcga cgccgctcag agcaacgata tcgccggcat acgccgaatc gacttccgta
     1441 cgcttcacgc cctgaaacgt gtacaggtgc gcgatctttc cgcgtaccgt cggattgttc
     1501 ggccgcacac ggtagacatc catcttgttc ttcagttcgc cggaaaacac acgcccgatg
     1561 gcgatccgac ccacgtagtc gttgtagtcg agcgtggtga cgagcatttt ca
//
LOCUS       DAORHD010001253         1611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102699_length_1611_cov_4.023136, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001253 DAORHD010000000
VERSION     DAORHD010001253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102699_length_1611_cov_4.023136"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1611)
                     /locus_tag="PKO06_20605"
     CDS             complement(<1..>1611)
                     /locus_tag="PKO06_20605"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNV72120.1"
                     /translation="RRLSREEGVPVPQLLRKVSGIGISSVGIAEDTLDSLASEGRIVL
                     WNGYELDRALERHASLSAELIDFVPRQEPIPGSLWAYSRDLSLLDRVERHIAEKAGRL
                     AVQRAGAHLLLIHRSGADFRGRFGLGFSTQALEQAYEADLGVVIRPYNVPRLGSGTIH
                     RLFQEFPHHERTSALLCADEEVLGHRGSLPTVAAELRRMPYRLGVVEFLDQSGMSELL
                     ALVGRSAPIVRVHSIGRRELDENYTPDRAVARWRRAVRERRLKMLYIRCFFENRKHLT
                     GDLLSSNLRYLENLVAGLKAEGFSIARTRLERQAEPRRDMPALSGFAGLALGQSLLLG
                     LTLLLALTRHQPFSPFLGLATIAASTLLFALLPKHLFLAVTGVAGAVGYATLGCIWAV
                     TALDDRLTFRAGLRALDFLRFVFRLALPAFVGGILIAGLYSDSDYLLKFEQFRGIKFS
                     LLLPVLWVGFWAFRRYGTGCVSLVFRHPTWLEMGIGALVLLAVSLYVVRSGNVTLLKP
                     SATEDLIRTWFEDAFIARPRNKEFLIGWP"
BASE COUNT          372 a          472 c          525 g          242 t
ORIGIN      
        1 cggccagccg atgagaaact ccttgttgcg cggacgcgcg atgaaggcgt cttcaaacca
       61 ggtgcgaatc agatcttccg tggccgatgg tttcagcagg gtgacatttc ccgagcgaac
      121 cacatacaga ctgacggcga gcaaaaccag ggcaccgatg cccatctcga gccaggtggg
      181 gtgacgaaac accagcgaga cgcaccccgt gccatagcgg cgaaacgccc agaacccgac
      241 ccacagcacg gggagcagga ggctgaactt gatgccacgg aactgctcga atttgagcag
      301 gtagtccgag tcactgtaca ggccggcgat cagaatgccg ccgacgaagg ccggcagggc
      361 cagacggaaa acaaaacgca gaaagtcgag cgcccgaaga ccggcgcgga aggtcagacg
      421 atcgtcgagt gcggtgacgg cccagatgca gcccagcgtc gcatagccga cggccccggc
      481 cacaccggtc acggcgagaa acaggtgttt cggcagcagc gcgaacagca gggtggaggc
      541 cgcaatggtt gccaacccga ggaatggaga aaatggttgg tggcgggtca acgccaagag
      601 cagggtcagg cccaaaagga gagactgtcc gagggcgaga ccggcaaatc cgctcagggc
      661 tggcatgtca cggcgcggct ctgcctgtcg ttcgaggcgg gtgcgggcga tgctgaagcc
      721 ttcggctttg agtccggcga ccagattctc gagatagcgc agattgctgg aaagcagatc
      781 gcctgtgaga tgcttgcggt tctcgaagaa acagcgaata tagagcatct tgagacgccg
      841 ctcgcgcacg gcacgtcgcc agcgggctac ggctcgatcc ggtgtgtagt tctcgtcgag
      901 ttcgcgacga ccaatgctgt gcacgcgaac aatcggagca gagcgcccca cgagtgccag
      961 caactcggac attccggatt gatcgagaaa ctcgacaacc cccagacgat acggcatgcg
     1021 tcgcagttca gctgcgaccg tcggaagcga gccgcggtgc ccgaggacct cttcatccgc
     1081 gcacagcagt gcggaggtac gctcgtgatg cggaaactcc tgaaacaggc gatgaatcgt
     1141 gccgctgccc agtcggggaa cattgtaggg acgaatcacg acgccgagat cagcttcgta
     1201 cgcttgttcc agcgcctgag tcgagaagcc gagtccgaag cgtccgcgaa agtcagctcc
     1261 tgaacggtga atcagcagca ggtgcgcacc ggctcgttgc acggcgagcc gtccggcctt
     1321 ttccgcgatg tggcgttcga cacggtcgag cagactcaga tcacgggaat acgcccagag
     1381 agaaccggga atcggctcct ggcgcggcac gaaatcgatc agctcggcgg acagggacgc
     1441 gtgacgttcg agggctcgat caagttcata gccgttccag agaacgatgc gcccttccga
     1501 ggccagggaa tcgagcgtgt cttcggcgat gcccacagaa ctgatgccga tgccggaaac
     1561 cttgcgcagt aactgaggga cggggactcc ctcttcgcga ctcaaacgcc g
//
LOCUS       DAORHD010001254         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_102889_length_1610_cov_2.384566, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001254 DAORHD010000000
VERSION     DAORHD010001254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102889_length_1610_cov_2.384566"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="rplC"
                     /locus_tag="PKO06_20610"
     CDS             complement(<1..546)
                     /gene="rplC"
                     /locus_tag="PKO06_20610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNV72121.1"
                     /translation="MAVKAILGTKIGMTQIFSETGDVIPVTVISAGPCTVIRKGEKAV
                     QVGYREINKDSVSRLLNKPLRLMFEKAGVKPFRIVRNLPADDTSAVETNTEITVSMFK
                     AGDMIDVIGTSKGKGFAGAMKRHNFGGGPVTHGQSDRTRCTGSTGSGQNASRTWKGKR
                     LPGQMGNVRKTIKGLRVVKVDE"
     gene            complement(564..875)
                     /gene="rpsJ"
                     /locus_tag="PKO06_20615"
     CDS             complement(564..875)
                     /gene="rpsJ"
                     /locus_tag="PKO06_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNV72122.1"
                     /translation="MAKQKMRLTLKAFDHRLLDQSVVRICDTVKRTGTRISGPVPLPT
                     EIRKYTVLRSPFVNKDSREQFEMRIHKRNIDLYSPSTQTVDSLMNMDLPSGVNIEIKM
                     L"
     gene            complement(953..>1610)
                     /gene="fusA"
                     /locus_tag="PKO06_20620"
     CDS             complement(953..>1610)
                     /gene="fusA"
                     /locus_tag="PKO06_20620"
                     /inference="COORDINATES: protein motif:HMM:NF015706.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV72123.1"
                     /translation="IRRLARAEVRHVKQLGGHGQYGHVIIEVEPLPRGGGFSFTDDTK
                     GDDVPRQFVKAIEQGCRDAMNDGALAGFTVVDVKVRLLGGSSHPVDSSDIAFKIAGFE
                     AMKKALATAAPVLLEPVMKVEIETPDQYMGDVIGNVNARRGKIVNVMMRDDVRVIDCT
                     APLADMFGYSTQLRSLTQGRATYSMELLHYEEVPDDVAQRMLGAWGYRPQAVQQAAAG
                     "
BASE COUNT          324 a          492 c          441 g          353 t
ORIGIN      
        1 ctcatcaacc ttgacaaccc gcagtccctt gatggtcttg cgaacattgc ccatttgccc
       61 gggaaggcgc ttgcccttcc aggtgcggct ggcgttctgc ccggaaccgg tggaaccggt
      121 acagcgggtt cggtctgact gtccgtgagt cacgggaccg ccaccgaagt tgtgtcgctt
      181 catggcaccg gcaaaacctt tgcccttgct cgtgccaatg acgtcgatca tatcgccggc
      241 cttgaacatc gaaaccgtga tttcggtgtt ggtttcaacc gccgaagtat cgtcagcggg
      301 aaggttccga acaatgcgga acggcttcac gccggccttc tcgaacatca ggcgcagagg
      361 cttgttcagc agacgggaga cggagtcctt gttgatctcg cggtacccga cctgaacagc
      421 cttttcgccc ttgcggatga cggtgcaggg gccggcgctg atcacggtga ccgggatcac
      481 atcaccggtc tcggaaaaaa tctgagtcat gccaattttc gttcccaaaa tcgccttaac
      541 ggccatggtg tcctcctgtt cccttacagc atcttgatct caatgttcac gcctgagggc
      601 aggtccatgt tcatgagaga atcaacagtc tgcgttgagg gactgtacaa atcgatgttt
      661 cgcttgtgaa tccgcatttc gaactgttcg cgggagtcct tgttcacgaa cggtgaccga
      721 agcacggtgt acttgcgaat ctcggtcggc aaaggaaccg gaccggaaat acgcgtgccc
      781 gtgcgcttga ccgtgtcgca aatgcgaacc acggactgat cgagcagccg gtggtcgaac
      841 gccttcagcg tcaaccgcat cttctgtttc gccatactaa ccacttcctc cttcggagtt
      901 cttaaaccgt gttatagcca cccgccgcct gggttcgcgg gtgaccccgc catcaccctg
      961 cggccgcctg ttggacggcc tgtgggcgat atccccaggc tcccagcatg cgctgggcaa
     1021 catcgtcggg aacttcctcg taatgaagca gttccatcga gtaggtggct cttccctggg
     1081 tgagcgaacg gagctgggtg gaatacccaa acatgtccgc caggggagcc gtgcagtcga
     1141 tgacccgcac atcatcgcgc atcatgacat tgacaatttt gccgcgccgg gcgttcacgt
     1201 tgccaatcac gtcacccatg tattgatcag gggtttcgat ctcaaccttc atgaccggct
     1261 caagcagcac cggagcggcc gtagcgagtg ccttcttcat ggcctcgaac ccggcaatct
     1321 tgaatgcgat gtcagagctg tctaccgggt ggctggaacc gcccaacaga cgcactttga
     1381 catcgacaac ggtgaaaccc gccagcgcgc cgtcattcat cgcgtcgcga cagccttgct
     1441 caatcgcctt cacaaactgt ctgggcacgt cgtcgccttt ggtatcatca gtgaacgaga
     1501 agccgccacc acgaggcaac ggctcgactt caatgatgac gtgtccgtac tggccatgcc
     1561 caccaagctg cttgacatgc cgcacttctg cgcgagccag tcgtcgaatg
//
LOCUS       DAORHD010001255         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_103150_length_1607_cov_3.841495, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001255 DAORHD010000000
VERSION     DAORHD010001255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103150_length_1607_cov_3.841495"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PKO06_20625"
     CDS             <1..1057
                     /locus_tag="PKO06_20625"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72124.1"
                     /translation="SKNPEEISLGIDALGKTGDERFWVNLRTFLGSEQPELRLGAMRS
                     MNRMVRIGEIHEHLGILQNLLRDPVRETRTLAIQIISRVRTPHSISLLVDALSDSSPR
                     NRRLAMEGLSFFGGEIVSPLMLLLDDPRASIPAQEAAVRLLNLSQDPDVHERLQRFAF
                     DRIKAMYEYKLDEQILRTESQLEDTDYLATVLDEKARMLLKLVLAHVAPDQRSSEARQ
                     VFKNLYSANQEMMSNAIEVLQSMGERTLIFHILPILEGLPLDQIASYGTRIFRLESRN
                     VQMVIGRYLLSLDPHLKEASVYTIGRLRLTELEPAVRKIQNSEKLSPELVHLIDWAID
                     QMTVRTRTWNAAGEKKP"
     gene            1054..>1607
                     /locus_tag="PKO06_20630"
     CDS             1054..>1607
                     /locus_tag="PKO06_20630"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV72125.1"
                     /translation="MITVEKILFLKRIPIFKSLNTTELRMIAEVTHEEEFAVGEVIFS
                     EGQQGDCMYFVFEGRINIFAGSPPKIQVLRVFETGDFFGEMGLFDDKPRAASAMAIDQ
                     ARVLVLRKGDFCELISEYPEVALSIMKELNQRIRATNQKLTTVEARLMDKTSQVYSRE
                     YFIECMFNIFLKAKKEEVSLAFLCL"
BASE COUNT          350 a          469 c          454 g          334 t
ORIGIN      
        1 cagcaagaac ccggaagaga tctcgctggg catcgacgca ctcgggaaga ccggcgacga
       61 aaggttctgg gtcaatctgc gaacctttct tggttctgaa cagccagaac ttcgtctggg
      121 agccatgcgc tccatgaacc gcatggtccg catcggtgag attcacgaac atctcggcat
      181 tctgcagaac ctcctgcgcg atcccgtgcg cgagacccgc acgctggcga ttcagatcat
      241 cagtcgcgtg cgtacgccgc acagtatcag tctgctggtc gatgcccttt ccgattccag
      301 cccgcgcaat cgtcgtctgg ccatggaagg cctgtcgttc ttcggcggtg agatcgtcag
      361 cccgctgatg ctgctgctcg atgatccgcg tgccagtatt ccggcgcagg aggccgcggt
      421 gcgcctgctg aatctctcgc aggaccccga tgtgcatgaa cgactgcagc gctttgcctt
      481 tgaccgcatc aaagcgatgt atgagtacaa gctcgatgaa cagattctgc gcaccgaaag
      541 ccagctggaa gataccgatt atcttgcgac ggtgctcgat gagaaggcgc gcatgctgct
      601 caagctggtg ctggcccacg tcgcacccga tcagcgcagt tccgaagcgc gacaggtctt
      661 caagaacctg tactcggcaa accaggagat gatgagcaat gccatcgagg tgctgcaatc
      721 gatgggtgag cgtaccctga tctttcacat tctgcccatt ctcgaagggt tgccgctcga
      781 ccagatcgcc tcgtatggca cccgcatctt ccgtctcgag agtcgcaacg tgcagatggt
      841 catcggtcgc tatctgctgt cgctcgaccc gcatctcaag gaagcctcgg tctacacgat
      901 cggtcgcctg cgcctgaccg agctcgaacc cgccgtacgc aagattcaga acagcgaaaa
      961 actttccccc gaactggtgc acctgatcga ttgggcgatc gatcagatga ccgtgcgcac
     1021 ccgtacgtgg aacgccgccg gagagaaaaa accatgatca ctgtcgagaa aattctcttt
     1081 ctcaaacgta ttccgatctt caagagcctg aatacgaccg agcttcgcat gattgccgag
     1141 gtgacccacg aagaggagtt tgccgtcggc gaggtcatct tctcagaagg gcagcagggc
     1201 gactgcatgt acttcgtatt cgagggacgc atcaacattt tcgccggcag tcccccgaag
     1261 attcaggtgc tgcgcgtgtt tgaaaccggg gacttcttcg gcgagatggg gttgttcgat
     1321 gacaaaccgc gggcggcatc ggccatggcg attgaccagg cgcgagtgct ggtgctgcgc
     1381 aagggcgatt tctgcgaact gatctcggaa tacccggaag tcgcgctcag catcatgaaa
     1441 gagctgaacc agcgcattcg tgccacaaac cagaagctga ccaccgtcga ggcgcgattg
     1501 atggacaaga ccagccaggt ctattcgcgc gagtatttca tcgagtgcat gttcaacatc
     1561 tttctcaagg ccaagaaaga ggaagtctcg ctggcctttc tctgtct
//
LOCUS       DAORHD010001256         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_103270_length_1606_cov_3.688588, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001256 DAORHD010000000
VERSION     DAORHD010001256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103270_length_1606_cov_3.688588"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PKO06_20635"
     CDS             <1..760
                     /locus_tag="PKO06_20635"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNV72126.1"
                     /translation="LASGTTLRVDLLNPAELGCPHHHTLTPGDLLKVQGGGVIAAIGV
                     GYEPYLERIRAAVPQVKIMEIASGAPLLPAVDVESEPFGNPHLFTCPSGLAHLTRALA
                     KRMREYAVAEQSIISHNESEVVKQLGQMEVEWQGIADSIVSMPVIVTHDSLDYLCRDL
                     GLGIVARLEESEGEGLSVARLLALKRDVSQKGVRAILVDSQHETKAAATIARETGVPV
                     LAVDTLTQGPTDGADLYLRLRRTAAQIRALSGLR"
     gene            771..>1606
                     /locus_tag="PKO06_20640"
     CDS             771..>1606
                     /locus_tag="PKO06_20640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNV72127.1"
                     /translation="MVHPLLTLENVGVRRGGEEVLTDVNCQIHRGMVTAIIGPNGAGK
                     TTLLQAILGLLPSTGRMRYFDGQGVERSGRPKIGYVPQRLGFDRALPITVLEFLALML
                     QRRPIWISIKERTRERAQEALQKLHASHLLTRPLEGLSGGEMQRVLLAAALMDEPELL
                     LLDEPVSAVDIAGGHLFCDVLEEAREKHRLTVVMVSHDLSVVSQHAQKVICLNRTVSC
                     QGDAPEVLSAEQLFAIYGVHSALYQHRPSHTQTACESCHECPQCPRSSGESGNARCDH
                     EH"
BASE COUNT          310 a          440 c          516 g          340 t
ORIGIN      
        1 cctggccagt ggcaccactc tgcgagtcga tctgctcaat cccgccgaac tcgggtgccc
       61 gcaccaccat acgctgactc cgggtgacct gttgaaagtg cagggtgggg gcgttattgc
      121 cgccatcggt gtcgggtatg agccgtatct cgaacgaatc agagcggcag tgccccaggt
      181 gaagatcatg gagattgcct caggtgcccc gctgctgccg gcggtcgacg tggaaagtga
      241 gccgttcgga aacccgcacc tgtttacctg cccatcgggg ctggcccatc tgacacgggc
      301 gctggcaaag cgaatgcgag agtatgctgt tgctgaacaa tcaataatct ctcataatga
      361 gagtgaagtt gtgaaacaac tgggacaaat ggaagtcgaa tggcaaggca ttgccgactc
      421 gatcgtttcg atgcccgtca tcgtcacgca tgattccctg gattacctgt gccgggatct
      481 tggtctgggt attgtggcac ggctcgaaga aagtgaaggt gaggggttgt ccgtggcacg
      541 acttctcgcc cttaaacggg atgtatcgca gaagggtgtc cgagcgattc tggttgactc
      601 acagcatgaa accaaggcgg cggcgaccat cgcccgcgag accggagttc ccgtgctggc
      661 ggttgacacc ctgacgcagg ggccaacgga cggagcggac ctttatctgc gcctgcgtcg
      721 aacggcggct cagattcggg cactctccgg tctgcgatga tatgagtgat gtggttcatc
      781 ccctgctgac actggagaac gtcggcgtgc gacgcggcgg tgaagaagtg ctgaccgacg
      841 tgaactgcca gattcatcgc ggcatggtca cggcgattat cggcccgaac ggggcgggga
      901 aaaccactct tcttcaggcc atacttgggt tgttgccctc taccggtcgc atgcggtact
      961 tcgatgggca gggcgtggaa cgttccgggc ggccgaagat cgggtatgtg cctcagaggc
     1021 tgggtttcga tcgagcgtta ccgatcacgg tactggagtt tctggcgttg atgctgcagc
     1081 gccgccccat ctggatcagc atcaaggaac gcacccgcga gcgcgcccag gaagcgcttc
     1141 agaagctgca tgccagtcac ctgctgactc ggccgctcga aggtctttcg ggcggcgaaa
     1201 tgcagcgtgt tctgctggcg gccgccctga tggacgaacc ggagctgttg ctgcttgatg
     1261 agccggtatc ggcggtggac attgcgggtg gtcatctgtt ttgtgacgtc ctggaagagg
     1321 cgcgtgaaaa acatcgtctg accgtggtca tggtcagcca cgatctctca gtcgtttcac
     1381 agcatgccca gaaagtgatc tgtctgaacc gcaccgtgtc gtgccagggc gatgcgcccg
     1441 aagtgctttc ggccgagcag ttattcgcta tttacggagt gcattcggca ttgtaccagc
     1501 atcgtccgtc gcatacgcag acggcatgcg aatcctgtca tgagtgtcct caatgtccgc
     1561 gctcgtcggg cgagagcggg aacgcccggt gtgatcatga gcattg
//
LOCUS       DAORHD010001257         1605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_103365_length_1605_cov_3.651613, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001257 DAORHD010000000
VERSION     DAORHD010001257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103365_length_1605_cov_3.651613"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PKO06_20645"
     CDS             complement(<1..1377)
                     /locus_tag="PKO06_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNV72128.1"
                     /translation="MNARLAVSPWGAGLDQVAAIRCLQVLDPRIFWLKPRRIETNAWE
                     LIRRADWFRPIEPGSVQWAALAEVHLVGVTQGRHIPDLLPLLGAVGGEIITYGHEDPR
                     LPAPSRHVRPRAFSLTAHFAAELARRNVPLGREDALLLAAAIWERTGGGLVSRTTDLD
                     WQVMDWLFRQRLPLLKIGNLVCPGWREGQRGLFNDLLRHMEDLEIRDWPVTLALVRSS
                     GHVQEVTPVVDALWSSTEPAMLVAGVCDAGRARIWVRSQTRFEELKKIFHDWSPVEQG
                     RWLTFRLPEGDPERVKECLRLCLEREYKPEMTAAEIMTSSPRCVEWHTVVADTYDMML
                     TFNIMGMPVLRDGLYAGFVTRRDVDRAIQMDLWDAPIGPHLSAEVPGISPDTPLRVIR
                     RLMVEHNQTKLAVVAEGMVVGLVTARELLRGLPDPVPLPRRFLPLVRAGEVPGPEQME
                     VLLKRVFSV"
     gene            complement(1374..>1605)
                     /locus_tag="PKO06_20650"
     CDS             complement(1374..>1605)
                     /locus_tag="PKO06_20650"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV72129.1"
                     /translation="GTGYPLGLKGDKIPLGARILSVADTFDSMIADRPYRKAFPFDYV
                     VREMKKVAGTQLDPHLVDVFLAILRRERKGKE"
BASE COUNT          352 a          525 c          452 g          276 t
ORIGIN      
        1 gaccgagaag acccgcttga gcagcacttc catctgttcg ggtcctggca cttcgccggc
       61 gcgaaccagg ggcagaaaac gacgcggcag aggcaccggg tctggcagcc cacgcagcaa
      121 ctcgcgcgcc gtcaccaggc cgacgaccat accttccgcg acgaccgcca gcttggtctg
      181 gttgtgctct accatcagac ggcgaatcac cctcaagggg gtatcggggc tgattcccgg
      241 aacctcggcc gaaagatgcg gaccgatcgg cgcatcccac agatccatct ggatggcgcg
      301 gtcgacgtct cgccgcgtga cgaaaccggc atacaggcca tcacgcagca ccggcattcc
      361 catgatattg aaggtgagca tcatgtcata ggtatcggcc acgaccgtat gccactcaac
      421 gcaccgcggt gatgacgtca tgatctcagc cgcggtcatt tctggcttgt actctcgctc
      481 aagacaaagt cgcaggcatt ctttcacccg ctccgggtcg ccttcaggca gacggaacgt
      541 cagccaacgc ccctgttcga ccggcgacca gtcgtgaaag atcttcttca actcctcaaa
      601 gcgcgtttgc gaacgaaccc agatgcgcgc ccgaccggca tcacagactc ctgccaccag
      661 catggccggc tcggttgacg accagagtgc atcgacgacc ggagtgactt cctgcacatg
      721 cccactgctg cgcaccaagg cgagggtcac gggccaatcg cggatttcca ggtcttccat
      781 atgtcgcagc aggtcattga acaatccacg ctgaccctcg cgccacccgg gacagaccag
      841 attaccaatc ttcaacaacg gcaagcgctg gcggaacaac caatccataa cctgccaatc
      901 aagatctgtc gtgcgtgaga caagtccacc gcccgtacgc tcccagatgg cggccgccag
      961 gagaagagca tcttctcgcc ccagagggac gtttcgccgc gccaattcgg cggcgaagtg
     1021 cgccgtcagt gaaaaggcac gggggcgcac atgccgtgat ggcgccggca gtcgagggtc
     1081 ttcatgacca taggtgatga tttcgccgcc gaccgcaccc aacagtggga gcaggtcagg
     1141 aatatgtctt ccctgggtta ctccgacaag atggacttct gcgagggcag cccactggac
     1201 acttcccggc tcgatcggac gaaaccagtc agcccggcga atcagttccc aggcgttggt
     1261 ttcgatgcga cgcggtttca gccagaaaat tcgcggatcg agcacctgca ggcagcgaat
     1321 ggccgcgacc tgatcgagcc ccgcccccca cggactgacc gcgagacgcg cgttcactcc
     1381 ttgcccttgc gttcccggcg tagaatggcc aggaatacat cgaccagatg cggatcgagc
     1441 tgggtgccgg caaccttctt catttcacgt acgacatagt cgaatggaaa tgccttgcga
     1501 tacgggcgat cggcgatcat cgagtcaaac gtgtcagcaa ccgacagaat acgggcgccc
     1561 agagggatct tgtcaccctt cagcccaagc ggatacccgg tacca
//
LOCUS       DAORHD010001258         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_103663_length_1602_cov_3.627666, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001258 DAORHD010000000
VERSION     DAORHD010001258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103663_length_1602_cov_3.627666"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="PKO06_20655"
     CDS             <1..607
                     /locus_tag="PKO06_20655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72130.1"
                     /translation="KHFTAALSSSADKLSISKNLGVIFMDKGLLDQAESEFKRAISIK
                     SDDPFTLNNMGKLLVLKNRREEGIEFFKRALLAQPDYGYAHYNLGKTYREIKDYPHAI
                     YHLLFCLKYEPEDIYAMNSLGRTFYEAGLHENAIEIFQRVIERHDEDDAYAVWGLAQC
                     HEAMGNLESALSHYQALQKLAEDNKALQEIARARVTALRGR"
     gene            607..1572
                     /locus_tag="PKO06_20660"
     CDS             607..1572
                     /locus_tag="PKO06_20660"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNV72131.1"
                     /translation="MLRRFDITVTDRWEEPLLGLAERLGVQCFSSPCLETRSGKGGEE
                     EIRLTGKTIVSFLASTVEQPIDQLEHSIRLWLQRNIGKAWGLSTKDYRDNEDWMQSFR
                     AYFQPIQISPHLWIRPPWHEPLTLGSECLQLQIDPGMAFGTGTHETTRLCLQLLGSLS
                     VTAGSFLDIGAGSGILAFYLLKSGARRVEAIEIDGPAIENLRKNAALNGIRKGLSIVQ
                     GDIYVYEPPWPIDGLVANISSPVLLDTFSRLEQWLPEKGWAVFSGVNSTNAPAVRQGL
                     KKAGLAIDREVVEGEWHGFLTSRATDVRTSSKRPRSPRRSRSPSS"
BASE COUNT          364 a          479 c          445 g          314 t
ORIGIN      
        1 gaaacacttt acggcagcgc tgtcttccag cgccgacaag ctgtcgatct cgaagaacct
       61 cggtgtcatc ttcatggaca agggcctgct cgatcaggcc gagtccgagt tcaaacgcgc
      121 catttcaatc aagagcgatg acccgttcac cctcaacaac atgggaaagt tgctggtact
      181 caagaaccgg cgcgaagaag ggatcgagtt cttcaaacga gcgctgctgg cgcaaccgga
      241 ttacgggtat gcccactaca atctcggaaa gacctaccgc gagatcaaag attatccgca
      301 tgcgatctat cacctgctct tctgtctcaa gtacgaaccc gaggatatct acgccatgaa
      361 cagcctcgga cgcaccttct acgaggccgg gctgcatgag aacgccatcg agatctttca
      421 gcgggtcatc gagcgtcacg acgaggatga cgcctacgcc gtgtggggcc tggcccaatg
      481 ccatgaagcc atgggcaatc tggaatccgc actctcgcat tatcaggcgc tgcagaaact
      541 tgccgaagac aacaaggccc tgcaggaaat tgccagggcc cgcgtcaccg cactgcgcgg
      601 ccgctgatgc tgcgccgatt cgacatcacc gtcacggatc ggtgggaaga gccgctgctc
      661 gggctcgcgg aacgcctcgg ggtccagtgt ttctccagcc cttgccttga gacacgctcc
      721 ggcaaaggag gcgaagaaga gattcgcctg acgggaaaaa ccatcgtcag ttttctggct
      781 tcgaccgtcg aacagcccat tgaccagctc gagcacagca ttcgcctgtg gctgcagagg
      841 aacatcggca aggcctgggg gctcagcacg aaagattacc gtgacaatga agactggatg
      901 cagtcgttcc gcgcctattt ccagccgatt cagatcagcc cgcacctctg gattcgacct
      961 ccctggcacg aaccgctcac actcggttcc gagtgtctgc agctgcagat cgacccagga
     1021 atggcctttg gcaccggtac gcacgaaaca actcggctct gcctgcagtt gctcggctct
     1081 ctctcagtca ctgccggctc ttttcttgac atcggtgccg gcagcgggat cctcgcattc
     1141 tatctgctga aatccggcgc gcgtcgggtc gaagccatcg agatcgatgg gccggccatc
     1201 gagaatctgc gcaagaatgc tgcgctcaac ggcattcgga aagggttgtc gattgttcaa
     1261 ggcgacattt atgtgtacga accgccgtgg ccaatcgacg ggttggtggc aaacatttct
     1321 tctccggtcc tgcttgacac attttcccgc ctggagcaat ggctgcccga aaaagggtgg
     1381 gcagtctttt ccggcgtcaa ctcgaccaac gccccggctg tgcgtcaggg tctgaagaaa
     1441 gccggtttgg cgatcgatcg tgaagtcgtc gagggagaat ggcatggctt tctgaccagt
     1501 cgggccacag acgtgcgtac aagttcaaaa cgtccgagaa gcccacgccg aagtcgatca
     1561 ccatcgtcgt aaacgacgaa attcaaaacc gccagaaacg ac
//
LOCUS       DAORHD010001259         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_103767_length_1601_cov_3.150712, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001259 DAORHD010000000
VERSION     DAORHD010001259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103767_length_1601_cov_3.150712"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>1601
                     /locus_tag="PKO06_20665"
     CDS             131..>1601
                     /locus_tag="PKO06_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72132.1"
                     /translation="MKHRTDGRIIAVFGFGEFIPVVTIKELHVVTRERGSDGTVVDAL
                     PFFLPGDVKHHQRPFALRLDLDSGDRLVLPLSSVAQPRPGTLVFAIDSANGSVVFNMA
                     SRGRTGWDELVYRITAEQASRMHVNVADGLAEARLRLSINRLELWKRGTGARMTVFGP
                     ERDRMEGYSVQWKTTSGEVTTSFRREQGEVVCDSPDDVALVEAVLINPRGVECGAIRA
                     DGAGKSLELYDPETKPTISIEAPPPMKAGAVVSLQAQGRGMPPTGPLSLNEAEYDTVW
                     KAGSPHAKVRTGEGKLIPQGRDRFVAIGHIELLRTVPPGGADVPVEMELFRRTSWREA
                     KPQNKLVGSARVIVKALHNPSLPAFTGQIQMVQTPGDPAGVGGPQDWDEGPPEVSLER
                     MTPIYGNNPFNTQNPIVNTTINGVTFTSTETVPVTSLPEPPKPATETAALQPPSPPQP
                     GTPSMTTSAIDRNDGDRVLPAAGGTIVDTVTYGNLIPGTA"
BASE COUNT          343 a          438 c          504 g          316 t
ORIGIN      
        1 tttctccact cgcggaacag cgccggagag tcttctgagt ctcacgttaa cgcgcgactg
       61 cctcgatctg gcatcggcag caaaaaaggg gacctggttc gatcggacac ccgttcgagt
      121 cgcggctcag atgaagcatc gcacggatgg tcggatcatt gccgtgttcg gctttggtga
      181 attcataccg gtcgtcacta tcaaagagtt gcacgtggtc acgcgcgaaa gaggatctga
      241 tggaaccgtt gtcgatgcct tgccgttctt cctgcctggt gacgtaaaac atcatcagag
      301 accatttgcg ctgcgcctcg acctggactc aggcgatcga ctggtgctgc ctttgagttc
      361 ggttgcgcaa ccacggccgg ggacgttggt gtttgccatc gacagcgcga atggctcggt
      421 agtcttcaat atggcctcac gcgggcgaac agggtgggat gaactggtgt atcgcattac
      481 cgcggagcaa gcctcgcgta tgcatgtgaa tgttgccgac ggcttggcgg aggcaagatt
      541 acggctgtcg atcaatcgac tcgaactctg gaaacgaggt acgggagcgc ggatgacggt
      601 gttcggcccg gagcgggacc gcatggaagg ctactcggtg cagtggaaaa ccacgtctgg
      661 agaagtgacg acctcatttc gccgcgagca gggagaagtg gtctgcgaca gcccggacga
      721 cgtagcgctg gtcgaagcgg ttctgatcaa tccgcgtggg gtggagtgtg gggcgattcg
      781 tgcagacggc gcgggaaaga gtcttgaact gtacgatccc gagacgaagc cgacgatctc
      841 gatcgaagct ccgccgccga tgaaggcagg tgcggttgtc agtctgcagg ctcaaggaag
      901 gggaatgccc ccgaccggcc cgctctcgct caacgaagca gagtatgaca cggtttggaa
      961 ggctggcagt ccgcacgcga aagtccgaac cggtgaggga aagctgattc cgcaaggtcg
     1021 cgatcgtttc gtggcgatcg gtcacatcga attgttacgc acggttccgc ctgggggcgc
     1081 ggatgttccg gtcgagatgg agctgtttcg aagaacctcg tggcgtgagg cgaagccgca
     1141 gaacaaactg gtcggttctg cccgggtcat cgtaaaagca ctacataatc cttcattgcc
     1201 ggcttttact ggtcagattc aaatggtaca aacaccgggt gatccggccg gggtgggcgg
     1261 ccctcaagac tgggacgagg gcccgcccga ggtctcgttg gagcggatga cgcccattta
     1321 tgggaacaac ccgttcaata cgcagaatcc gatcgtcaac acgacgatca atggagtgac
     1381 cttcacgtcg accgagaccg tacccgtaac ctcgttacca gaaccgccaa agcctgcaac
     1441 ggaaacggct gctctgcaac caccgtctcc gccgcaacct ggcaccccct ctatgaccac
     1501 ctctgccatt gatagaaacg atggcgaccg agttttgcca gcggccggcg gaacgatcgt
     1561 ggataccgtg acgtatggca atctgattcc gggaactgcc t
//
LOCUS       DAORHD010001260         1599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104004_length_1599_cov_2.600389, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001260 DAORHD010000000
VERSION     DAORHD010001260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104004_length_1599_cov_2.600389"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKO06_20670"
     CDS             complement(<1..185)
                     /locus_tag="PKO06_20670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72133.1"
                     /translation="MKHWKHLLVGAGIAFLSVLPPVNALEVGQYQSILRQKNPPTQTR
                     ASGSLLPPSPEPRRPNAP"
     gene            334..>1599
                     /locus_tag="PKO06_20675"
     CDS             334..>1599
                     /locus_tag="PKO06_20675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV72134.1"
                     /translation="MKLVRHLHRVDLILLGCWTLFLLVIGWQDWQNHQRTTFHNAEIE
                     AAAHLALNVAYRTTISNMGGLYADTRRVEPNPYLRVSHRDLQISATETLTLVNPAYMN
                     RLVFRDMASAGLEIVNKITSLKPINPDNAPDSWETATLRSFETGASQAVEVAPLRGRM
                     FLRLMKPFPVVESCLRCHADQGYKVGDIRGGISISIPTDAYQVVEQRATRMTFLNLGM
                     IWVLGCSAMIWAFWKRRLQDLSLLEKERQFRLLSESAQDWELWLTPEGRHLFVSPSVK
                     RLTGYDPEEFFRDPDLLNKLVHGDSQSVWQKHRADCNDSRESQEIELSIVMRDGRQRR
                     FLHLCAPIISDGVCLGRRITHRDITERRQLEDRLRHAQKMEAVGQLAGGIAHDFNNIM
                     AAVMGYANLIEMELVDRPDIMARVRKIVES"
BASE COUNT          331 a          447 c          470 g          351 t
ORIGIN      
        1 ggagcgtttg gacggcgagg ttcgggcgac ggtggcagaa gcgaaccgct cgccctggtc
       61 tgcgtcggcg gattcttctg gcggagaata ctctgatact ggccgacctc gagagcgttc
      121 acgggcggca gaacgctcag aaacgcgatc cccgcgccga ccagcagatg tttccagtgt
      181 ttcatgatag tgtctccccg cctcgttatc gtcacgttcc gtctgttttc ttagtcttca
      241 tgcacgagtt tcaggcgtac agtgaaaaac gatacgcgac tgttcgtgat tggggtataa
      301 tgaataaaag tggatctttc gggagcgctg ccagtgaaac tcgtccgaca tcttcaccgc
      361 gtggatctga tcctgcttgg atgctggacg ctctttctgc tcgttatcgg ctggcaggat
      421 tggcagaacc accagcggac gacgtttcac aatgccgaaa tcgaggctgc ggcgcatctg
      481 gcattgaatg tcgcctatcg cacgacgatc tcgaacatgg gcgggttgta cgctgatacc
      541 cgtagggtgg aaccgaaccc gtatcttcgg gtctcgcacc gtgatcttca gatctcggcc
      601 accgagaccc tgacgctggt gaacccggct tacatgaatc ggttggtgtt ccgcgatatg
      661 gcatctgccg gtctcgagat cgtcaacaag atcaccagtc tgaaaccgat aaatccggac
      721 aatgccccag actcatggga aacagccacg cttcgaagtt tcgagaccgg agccagtcag
      781 gccgtcgagg tggctcctct gcgtggtcgt atgttcttga gactgatgaa accgttcccg
      841 gtggttgagt cctgcctgcg ctgccatgcc gatcagggat ataaggttgg cgatattcgc
      901 ggcggcatca gcatctccat tccgaccgat gcataccagg ttgttgagca acgcgcgacg
      961 cgcatgacct tcttgaacct cggcatgatc tgggtgctcg gatgcagcgc catgatctgg
     1021 gccttctgga aacgtcgcct gcaggacctc tccctcctcg aaaaggagcg ccagtttcgc
     1081 ctgctgtcag aatccgcgca ggactgggaa ctgtggctca ctccggaggg gcgtcacctt
     1141 ttcgtatcgc cctcggtgaa acgactcacc ggatatgacc cggaggaatt ctttcgtgac
     1201 cccgacctgt tgaacaaact tgttcatggc gattcccagt ccgtgtggca gaaacatcgc
     1261 gccgactgca acgacagccg ggaatcacag gaaatcgagc tgagtatcgt gatgcgggac
     1321 gggcgacagc gtcggttctt gcatctctgc gccccgatca tctccgacgg agtctgtttg
     1381 ggtcggcgta tcacgcatcg agacattacc gaacgtcggc aactcgaaga tcgcctgagg
     1441 catgcacaga aaatggaagc ggtcggtcag cttgcgggcg ggatcgcgca cgatttcaac
     1501 aacatcatgg cggcagtcat ggggtatgcg aacctgatcg agatggaact cgtcgacaga
     1561 cccgatatca tggcccgggt gcgcaagatc gtcgagtcg
//
LOCUS       DAORHD010001261         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104206_length_1597_cov_2.875486, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001261 DAORHD010000000
VERSION     DAORHD010001261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104206_length_1597_cov_2.875486"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1597)
                     /gene="eutB"
                     /locus_tag="PKO06_20680"
     CDS             complement(<1..>1597)
                     /gene="eutB"
                     /locus_tag="PKO06_20680"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HNV72135.1"
                     /translation="NSPTDNPEDIVWQVFSGWSYGTGDVVLGTNPVSGLIEDLRTVEN
                     SLKDLIDTFKLAKVLPWCVLAHIDKQAEVEKTFPGTTALWFQSLASCDDANKTFDVSV
                     DKMRTYAAQRKGPYGMYFETGQGADFTNGAGHGFDMVIHESRKYGFARALNHEMAKVV
                     PDGKVWTITNDVAGFIGPEVFRSREQLVRCCLEDIVMGKLHGLCIGLDICSTLHMPIS
                     LDDLDWCMDQIMPANPGYLMALPTKNDPMLSYLTTAFQDHVRLREKFGYRVNDPMVEF
                     FKKIGILNQEGKYTEHFGDPLWVYYQYKLVKGDKRSQQDIYAEGEQAIQRCEARGVPI
                     ARGYGRERWNIHPPLAKKIINLYNDAKECIWDEFSPEFIARFPRALVISTQSKNREDY
                     IAHPSSGEKLSPEAVTAVEQLRDSWKGKIPDVQIVISDGLNSRSITDDGHLEPYLTTV
                     RKQLTEAGLTVADQPIIVKSGRVRAGYAIGNLLFAKADPQTPRILLHIIGERPGTEHR
                     NFSVYLAGPKAGIWASGKVDHDIA"
BASE COUNT          325 a          427 c          468 g          377 t
ORIGIN      
        1 gagcgatgtc gtggtcgact ttgcctgatg cccagatccc tgccttcgga cctgcgagat
       61 agacagaaaa gttgcggtgt tcggtgcccg gtcgctcgcc gatgatgtgc agcaggatcc
      121 gaggtgtctg aggatctgct ttggcgaaca gcaggttgcc aatggcatac ccggcccgaa
      181 ctcgacctga cttgacgatg atcggttggt ccgcaacggt cagcccggcc tccgtcagtt
      241 gtttccgaac ggttgtcaga tagggctcca gatggccgtc atcggtgatc gaacgactgt
      301 tgagaccatc tgaaatgacg atctgtacat ctggaatttt ccctttccag gaatcgcgca
      361 gctgttcgac ggcggtgacg gcttcagggc tcagtttttc cccgctggaa ggatgggcga
      421 tgtagtcctc gcgattcttc gactgtgtcg agatcaccag agcccgcggg aatctggcga
      481 tgaactcagg tgaaaactcg tcccagatgc actcttttgc gtcattgtac aagttgatga
      541 ttttcttggc caggggtgga tgaatgttcc agcgttcgcg cccgtaaccg cgcgcgatag
      601 gaacgccgcg ggcctcacat cgttgaatcg cctgctcgcc ttccgcgtag atatcttgct
      661 gagagcgctt gtcgcctttg acgagcttgt actgatagta gacccagagg gggtcgccga
      721 aatgctcggt gtattttccc tcctgattca ggatgccgat tttcttgaaa aactcgacca
      781 tgggatcgtt cacgcggtaa ccgaacttct ctcgtaagcg cacgtgatcc tgaaaagcgg
      841 ttgtcaggta actcagcatc ggatcgttct tggtgggaag agccatcaga tagccggggt
      901 tggcgggcat gatctgatcc atgcaccagt cgagatcgtc gagcgagatc ggcatatgca
      961 gagttgaaca gatatccaga ccgatgcaga gaccatgcag tttgcccatg acgatgtctt
     1021 cgagacagca gcgtaccagt tgctcgcgag acctgaagac ttccggtccg atgaagcctg
     1081 ccacgtcatt ggtgatcgtc cagactttgc cgtcgggaac gactttggcc atttcgtggt
     1141 tcaaagcccg ggcaaagccg tacttgcgtg attcgtgaat caccatgtcg aatccgtggc
     1201 cggcgccatt cgtgaaatca gcgccctgcc cggtttcgaa atacatgcca tacggcccct
     1261 tgcgttgggc cgcataggtg cgcatcttgt cgacgctgac atcgaaggtt ttgttggcgt
     1321 catcgcaact ggcgagactc tgaaaccata acgccgtggt tcccggaaac gtcttttcga
     1381 cctcggcctg cttgtcgatg tgtgccagca cgcaccaggg aaggactttg gccagcttga
     1441 aggtgtcgat cagatccttg agggagtttt ccacggtgcg aagatcctcg atcaacccgg
     1501 agacggggtt ggtgccgaga acgacgtcgc cggtcccgta actccagccg gaaaagacct
     1561 gccagacgat gtcttcagga ttgtcagtcg gagagtt
//
LOCUS       DAORHD010001262         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104215_length_1597_cov_2.734760, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001262 DAORHD010000000
VERSION     DAORHD010001262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104215_length_1597_cov_2.734760"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1597)
                     /locus_tag="PKO06_20685"
     CDS             complement(<1..>1597)
                     /locus_tag="PKO06_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72136.1"
                     /translation="LGEDPPNQAIIGMHEERRVVSINPLGRRSYFYGYTDHRGTLIAH
                     VNSGAVPEWYTMRAFAERARRKLPQCAFGVWILGEELSSPSMKRNEKQTIMRALAELE
                     LSMTPWIERDGLLIRSHSLDASYRFWGVRRLNLELQGLTLRARVELYSLLFFLMLAGF
                     TFSWLVQGWRLRVSIRWKLMLLFGIASGLPLILLLVTGYDYLVRTEQVLKFETLRKLQ
                     DSLKMVDSRFPRFLHDCRKRLMGVTAAAKALQPVDLQRFVDSLDEMREELGFFTPVVV
                     KSDGTVYQGHKSGNEKSGDRLMLALAFEMMRRYNRNLGYPEDVGGKAVKSVEMALLND
                     VQIGGAVEGLFRGLGKFTRIHFGKNSRLVLLDFFQGENGRADALMMLAFATDRVFYRF
                     LMKYQARYQKAFGFGTLYSFHERKQFMVPEDAFKAEWLDNFRLRMLTRREVISEEQTI
                     QGVPHLIVGMPGKEMESFMLVQTIPTAAVTGVKEELKTRLTWLGMICLALALASGLLL
                     AGQFLQPIASLSEGVQAIQAGKFR"
BASE COUNT          383 a          503 c          408 g          303 t
ORIGIN      
        1 cggaactttc cggcctgaat cgcctgaacg ccttcggaca acgaggcaat cggctgcaga
       61 aactgaccgg cgagcaacaa cccagaagcc agagcaagcg cgagacagat cattcccaac
      121 caggtgagcc tcgtcttcag ttcttccttg acccctgtca cggctgccgt cggaatcgtc
      181 tgcaccagca taaagctttc catttccttg ccgggcatgc cgacgatgag gtgcggaacc
      241 ccctgaatcg tctgttcttc ggaaatgacc tcacggcggg tcagcatccg caatcggaag
      301 ttatcaagcc actcggcttt gaaggcatct tccggcacca tgaactgctt gcgctcgtga
      361 aaagagtaca aggtgccaaa accgaaagcc ttctgataac gggcctgata cttcatcagg
      421 aatcgataga acacccgatc ggtggcgaaa gccagcatca tcaacgcatc agcccgaccg
      481 ttttctccct ggaagaagtc gagcagcacc agacggctgt tcttgccgaa gtgaatgcgg
      541 gtaaacttgc ccagaccgcg aaacagccct tcgaccgcac cgccgatctg gacgtcgttg
      601 agcagtgcca tctcgaccga tttcaccgcc ttgccgccca catcctccgg atacccgagg
      661 ttgcggttgt aacgacgcat catctcaaac gccagggcca gcatcagccg gtcacccgat
      721 ttctcgttac cgctcttgtg cccctgatac actgttccgt cagatttgac caccacggga
      781 gtgaagaacc caagctcttc acgcatctcg tcaaggctgt cgacgaaacg ttgcagatcg
      841 accggctgca gggccttggc cgccgccgtg acccccatga gacgttttcg acagtcatgc
      901 agaaaccgcg gaaagcgcga gtcgaccatc ttcagtgaat cctgcaactt gcgcagcgtc
      961 tcaaacttga gcacctgttc cgtgcgaacg agatagtcgt atccggtcac caacagcaga
     1021 atcaacggca accctgaagc aatgccgaac agcagcatca gcttccagcg aatcgacaca
     1081 cgcagtctcc atccttgtac cagccatgag aacgtaaaac cagccagcat caggaaaaac
     1141 aggagtgaat acagttcaac acgggctcga agcgtcagcc cctgcaactc cagattcaga
     1201 cgccggacac cccagaatcg gtaggaagcg tccaggctgt gagaacgtat cagcagccca
     1261 tcgcgctcga tccagggggt catggaaagc tccagttcgg cgagcgccct catgatcgtc
     1321 tgtttctcgt ttcgcttcat gctgggagaa ctcagttcct caccgaggat ccagaccccg
     1381 aaggcacact gtggcaactt gcggcgcgct cgttcggcaa acgcgcgcat cgtgtaccat
     1441 tcaggaaccg cgccactatt gacgtgagcg atcaacgtcc cccgatgatc ggtgtagcca
     1501 taaaaatagc tccggcgccc gagagggttg atcgagacca cgcgccgctc ttcgtgcatg
     1561 ccaatgatgg cctgattcgg cggatcttcg ccgagaa
//
LOCUS       DAORHD010001263         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104284_length_1596_cov_4.141467, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001263 DAORHD010000000
VERSION     DAORHD010001263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104284_length_1596_cov_4.141467"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKO06_20690"
     CDS             <1..233
                     /locus_tag="PKO06_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72137.1"
                     /translation="ESYDSGEDRKTVDRLKPLLHEPRLFLRTFVLQGLVIDCPSREVR
                     LEYLTPWDPEHTRLAVLDWSFQIKRYGLSCEL"
     gene            352..885
                     /locus_tag="PKO06_20695"
     CDS             352..885
                     /locus_tag="PKO06_20695"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNV72138.1"
                     /translation="MPINEKVAKLMNEQIMKEWYSAYLYMSMAGYLEVQNLPGMAHWM
                     QIQVLEELSHGRIFQKYLSEDMGIQPVLEPIAAPPKTFSSPQDVFEKAYEHELTVTAS
                     IHNIMDHAIKAGDHASRHMLEWFVAEQVEEEKSASEIVAMFKRMPKGHEAILFQLDAK
                     LAARTFTMPPQLAKGAA"
     gene            1006..>1596
                     /locus_tag="PKO06_20700"
     CDS             1006..>1596
                     /locus_tag="PKO06_20700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNV72139.1"
                     /translation="MLYRTMPRSPEPLSILGFGCMRFPEKNGKIDEEAAERQIRRAIE
                     RGINYFDTAAVYHMGASEPFLGRVLKKDGLRDKVFLATKLPHWNAQSRADMDRLLDMQ
                     LQKLQTNRIDYYLVHNLTAPTWQRMHELGILDFLESAKRDGRIRYAGFSYHGDRDTFP
                     RIVDAWNWDFCQIQYNFLDEQTQAGRAGLEYAARKNL"
BASE COUNT          362 a          476 c          428 g          330 t
ORIGIN      
        1 ccgaatccta cgactctggc gaagaccgaa agaccgtcga tcgcctgaaa ccgttgcttc
       61 acgagccgcg actgtttctg cgtaccttcg tgcttcaggg tctggtgatc gactgtccgt
      121 cacgggaagt gcgactcgag tatctcaccc cctgggatcc ggaacacacg cgactggccg
      181 tgctcgattg gtcttttcag atcaagcggt acggtctgag ctgcgaactg taaggcaaag
      241 tccggcacac tggccctttt atgctccttt ctcaggagca atttgacatc ccgtgacgat
      301 tcaggtaagg tgaagaagtt cgcagatctc atcaccacaa ggagagagac gatgccgatc
      361 aatgagaaag tcgccaagct catgaatgag cagatcatga aggaatggta ttccgcgtat
      421 ttgtacatgt cgatggccgg gtatctcgaa gtgcagaacc tgccgggtat ggcgcactgg
      481 atgcagattc aggtgctcga agaactctcg cacggccgca tcttccagaa gtatctgtcg
      541 gaagacatgg gcattcagcc ggtgctcgaa ccgatcgccg cgccccccaa gacgttcagt
      601 tccccacaag acgtcttcga gaaggcctat gagcatgaac tgaccgtgac cgcgtcgatt
      661 cacaacatca tggatcacgc catcaaggcg ggtgaccacg cttcgcgcca tatgctcgag
      721 tggttcgtcg ccgagcaggt cgaagaggaa aagagtgctt ctgagatcgt agccatgttc
      781 aagcgcatgc cgaaagggca cgaagccatt ctctttcagc ttgatgccaa actggcagcc
      841 cgcacgttca cgatgccccc gcagctggcc aagggcgctg cctgagccga ttccgctcac
      901 acgttcactc acgaacgcga ccctctctcg ggtcgcgttt ttctttgtgc tatgctgtca
      961 ccatcttccc aaccaccgcg ccacgctcaa caaggagaag cactcatgct gtatcgtacc
     1021 atgccacgct cgccagaacc cctttcaatt ctcggttttg gctgcatgcg tttcccagag
     1081 aagaacggca agatcgacga ggaagcggcc gagcgccaga ttcgccgagc gatcgagcgc
     1141 ggcatcaact acttcgacac ggcagccgtc tatcacatgg gtgccagcga gccctttctc
     1201 gggcgcgtgc tgaagaagga cggtctgcgc gacaaggtct ttctcgccac gaaactgccg
     1261 cattggaacg cgcaatcgcg ggccgatatg gatcgcctgc tcgacatgca gctgcagaaa
     1321 ctgcaaacca atcgcatcga ctattatttg gttcataatc tgaccgcccc cacgtggcaa
     1381 cgcatgcacg agctcggcat tctcgacttt ctcgagtcag ccaaacgcga cggtcgcatt
     1441 cgctacgccg ggttttcgta tcacggcgat cgcgacacct ttcccaggat cgttgacgcc
     1501 tggaactggg atttctgcca gatacagtac aactttctcg acgagcagac acaggcggga
     1561 agagccggtt tggaatacgc cgcgcggaaa aacctc
//
LOCUS       DAORHD010001264         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104318_length_1596_cov_2.979234, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001264 DAORHD010000000
VERSION     DAORHD010001264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104318_length_1596_cov_2.979234"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKO06_20705"
     CDS             <1..385
                     /locus_tag="PKO06_20705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72140.1"
                     /translation="KGSIQMPTTEWYLVEELELLPKKKWEDMADDAEDEDEEEEELDD
                     EEELDDEEEEGDEDLDDEEFDDEDEDFEDFDDDDEELEDEEEEDEDFDEEDEDFDDDD
                     DFEDDEEEELDDDFEDDEEEEEDEE"
     gene            complement(661..1269)
                     /locus_tag="PKO06_20710"
     CDS             complement(661..1269)
                     /locus_tag="PKO06_20710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72141.1"
                     /translation="MVRKRTCELPFCHGLVEGTRNKRRCDKDRNIHLHQIISKNVKKI
                     MRYIESHREFTANDVARDLFKNKLDKNEKGSMYRLFQAMVRENYLTEHGKLDGLKVYC
                     FAGADDIYAPLSPEPAAAQLGAASYPQTPAPVEVPLTPIELLTAKLREFIFLKNGEYH
                     HEDWLWLINRPDIRELSLKLNMDEGQLGILLQGEKDRYWARR"
BASE COUNT          400 a          400 c          451 g          345 t
ORIGIN      
        1 aaaggggtcc atccagatgc ctaccacaga atggtacctt gttgaagaac tcgaacttct
       61 gccgaaaaag aagtgggaag atatggccga cgacgccgag gacgaagacg aagaagaaga
      121 ggaactcgac gacgaagaag aactcgacga cgaggaagaa gaaggcgacg aggacctcga
      181 tgacgaggaa ttcgacgacg aagatgaaga cttcgaagac ttcgacgacg atgacgaaga
      241 gctcgaagac gaagaagaag aagatgaaga cttcgacgaa gaagacgaag acttcgatga
      301 cgatgacgat ttcgaagacg acgaagaaga agaactcgat gacgacttcg aagacgacga
      361 agaagaagag gaagacgaag aatagtctgc tcgcacagat ctgacgcgta atgaaaacgc
      421 cccacctttg tggggcgttt ttcgtcgaga agtttttccc aggcatctcg agtttgtcat
      481 gggtgtcgtg cgcgcatcgt gtgtgttggt ctcttcagac tcgtgatccc ttgtcctcac
      541 tggcgatcgc aggggcgcca ccgaactctt gtcgctgaca acggctccgg gaaaagaaaa
      601 cccgccacct cgaaaagcac ggtgtggcgg gcgtgagaga cgaacgatcg aaagggtcga
      661 ttaacggcgg gcccagtagc ggtccttttc gccctgcagc agaatgccga gctggccttc
      721 atccatgttc agcttcagag acagctcgcg aatgtctggc cggttgatga gccagagcca
      781 gtcttcgtgg tgatactcgc cgtttttcaa aaaaatgaac tcgcgcagtt tggccgtcag
      841 aagctcgatc ggagtcaatg gaacttcgac cggtgccggg gtttgtggat acgatgccgc
      901 cccgagttgg gccgcagccg gttctggaga aagcggggca tagatgtcgt cagcgccggc
      961 gaagcagtag accttcagcc cgtcgagctt gccatgttcg gtcagatagt tttcgcgaac
     1021 catggcctga aagagtcgat acatcgaccc cttctcgttc ttatcgagct tgttcttgaa
     1081 gaggtctcgg gcgacatcgt tcgccgtaaa ctcacggtgc gattcgatgt agcgcatgat
     1141 cttcttcacg ttcttggaaa tgatctggtg caggtggatg ttgcgatctt tgtcgcagcg
     1201 ccgcttattg cgggtaccct caaccagacc atgacaaaaa ggaagttcac acgtacgttt
     1261 tctaaccatg gtctccctca actgcttctg tttctcttgc gtggggcacg gtatcccgta
     1321 ccttctcgag ccgagcgtag tttactgaag gtcttttact gttgtcaaac ttttttctgc
     1381 actgtaggaa cgacgctttc agtatgaatg acgtgcccca tgtcgcgggc gaatgcacga
     1441 cgcctcaggc tcgcaaccca tgattttacg gcgttgcgca acgtgatgtg aaagagtata
     1501 attccctcgc gttcaagtcc tcgactccag gagtccccat gccgcaacta cgcaaagatc
     1561 ccgtgaccaa gcgctgggtc atcattgtac acgaac
//
LOCUS       DAORHD010001265         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104319_length_1596_cov_2.907203, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001265 DAORHD010000000
VERSION     DAORHD010001265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104319_length_1596_cov_2.907203"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKO06_20715"
     CDS             <1..199
                     /locus_tag="PKO06_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72142.1"
                     /translation="GLLNLEWLYPLAGEQGKRRKYLLIGFLIVIASVIGLHTIFSFST
                     RVFHATSGVGGPPGLWKFLGE"
     gene            complement(272..>562)
                     /locus_tag="PKO06_20720"
     CDS             complement(272..>562)
                     /locus_tag="PKO06_20720"
                     /inference="COORDINATES: protein motif:HMM:NF023945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72143.1"
                     /translation="PLIVAGGHDPLLEWALREMGRRCADRLIQIDDTVRKRIIDRFKK
                     ENRKRGFIAPLQEVHHLEPEELTEFLGEALPSGFIWARDCDDVTPEASATGG"
     assembly_gap    563..572
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<573..>1596)
                     /locus_tag="PKO06_20725"
     CDS             complement(<573..>1596)
                     /locus_tag="PKO06_20725"
                     /inference="COORDINATES: protein motif:HMM:NF023945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72144.1"
                     /translation="AFYLYYSGNPPRGEGAGDVVAYQSRRHRPLPPLRLLTAGTKGSA
                     SAGRVEEVQIAAKLRETGFLQILCRHCADGREEELAFDLSQSSEKEKRVSSSEKTAKA
                     TPGKRQVGMLKKYIEAMFDEKHPVPFSQVFKKLEEMLGQPRQEWDLPLLRSLFDLLID
                     DERFQRSSEGWQAWFRLCGFALRPGYGFSGDDERVARLWQMHEPPFATKESAFWAEWW
                     ILWKRLAPGLGADRQAVLTQMAEALLFGKVTGKGGRVVEQHERNQLWRLLGQLERLPA
                     TEKERLGSWILKAPTTFSRDAMALMALGRLGARVLAYADHTHLVPRTTAQSWVESLLP
                     RTIAGSA"
BASE COUNT          293 a          517 c          459 g          317 t
ORIGIN      
        1 gggattgctc aacctggagt ggctctaccc gctcgccgga gagcagggca aacgtcgtaa
       61 gtacctgttg atcggctttc ttatcgtgat tgccagtgtc attggcctgc atacgatttt
      121 cagtttttcg accagggttt ttcacgccac cagcggcgtc ggcggtcccc ccgggctctg
      181 gaaatttctg ggagagtaac cagaatcgcg gctcctgccg gtcggggaga tctgaagcgc
      241 acgtggggtt gttccgggaa agcgtgggga gttacccgcc ggtcgcgctc gcttcgggcg
      301 tcacgtcgtc gcaatcacgg gcccagatga accctgacgg caaggcctcg cccaggaact
      361 cggtcagttc ctctggctcc agatggtgga cttcctgtaa cggggcgatg aagccgcgtt
      421 tgcggttttc tttcttgaac cgatcaatga tgcgtttgcg caccgtgtcg tcgatctgaa
      481 tcagccgatc cgcgcagcgg cgacccattt cgcgcagggc ccattccagc agggggtcat
      541 ggccgcccgc cacgatgagg ggnnnnnnnn nnaagccgac ccggcaatcg tccgcggcag
      601 caggctttcg acccagctct gcgcggtcgt gcgcggcacc agatgcgtgt gatcggcata
      661 agccagaacc cgcgccccca gccgcccgag agccattaac gccatggcat cacgggaaaa
      721 cgtggtcgga gccttgagaa tccaggagcc gaggcgttcc ttctctgttg ccggcagacg
      781 ctcgagctgt cccagcaaac gccatagctg gttccgctcg tgctgttcca cgacgcgccc
      841 tcccttcccg gtcacctttc cgaagagcag cgcttcggcc atctgcgtca gcacggcctg
      901 gcgatccgct cccaggccgg gggccagtcg cttccagaga atccaccatt ccgcccaaaa
      961 tgcgctttct ttcgtggcaa atggcggctc atgcatctgc cacaaccggg cgactcgctc
     1021 gtcgtccccc gagaacccat atcccgggcg cagcgcaaaa ccgcacaacc gaaaccacgc
     1081 ctgccacccc tcgctcgagc gctgaaaccg ctcgtcgtca atcagcaggt cgaacagact
     1141 ccgcaatagc ggcagatccc actcttgccg gggctgcccc agcatctctt cgagtttctt
     1201 gaaaacttga gaaaaaggga cagggtgctt ctcatcgaac attgcttcga tgtacttttt
     1261 cagcatgccg acctgccgtt tgccgggagt ggctttcgca gtcttctccg agctgctgac
     1321 acgcttttcc ttctcagacg actgggacaa atcgaacgcg agttcttcct cgcgaccatc
     1381 ggcgcagtgc cggcacagaa tctgcaagaa accggtttca cgcagtttgg ccgcgatctg
     1441 cacctcttcg acgcgcccag ccgaagcgct cccttttgtt ccggcggtca gcagccgcag
     1501 gggcggcaga ggtcgatgac gccgcgactg ataggcgacg acatctcctg cgccttcgcc
     1561 gcgcggcgga ttgccggagt agtacaaata gaaagc
//
LOCUS       DAORHD010001266         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104396_length_1595_cov_4.291558, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001266 DAORHD010000000
VERSION     DAORHD010001266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104396_length_1595_cov_4.291558"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..850
                     /locus_tag="PKO06_20730"
     CDS             176..850
                     /locus_tag="PKO06_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72145.1"
                     /translation="MRDTWNRPSLWGVMALVVLITAALGETAPRVGWSLRLDIGGRGG
                     WPEPVWVASDLEMLVRRLERDREAKIAEADRQGVFYRPPPLGTFPSWTRCQELALQHR
                     PEMILIRVNMAGLKAARRLLKGGYGDSDEDSLGRNLPSWFSAERWGEEILRDFRLSGR
                     SREKISRLLSQQLDGVTALFTEWRDRLNHEAAQASHSLERARLKGSEDAVRLACWKLW
                     ITMGCP"
     gene            1099..>1595
                     /locus_tag="PKO06_20735"
     CDS             1099..>1595
                     /locus_tag="PKO06_20735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72146.1"
                     /translation="MSRFIETLRLSHLLPLIEQEFRKVPDLREAEASALCHSMSDTLM
                     AGLAMMFVQDPSMLAFQARLQEKQRGNNLRTIFGIEKVPKASQLGRILDCADPQTLQR
                     AFTPVLQCLQKTRLWSNFRVLGGYYAVLIDGFEYFRSEKRGCKHCQEYHHRDGRVEHA
                     HQVLAA"
BASE COUNT          324 a          404 c          476 g          391 t
ORIGIN      
        1 ctagtatcag caccgttttc ccttcgcctt cgctcaacgc ttcgccgttg cgctcaggcc
       61 atgacagacg tattgtgagt tgaggggttc gcgaacttct gttatcagcc agagcggggg
      121 ttgtgatcca ggacaagaat tgggcaagag aattggtcga gagaggagag tgttgatgcg
      181 agatacgtgg aaccgtccga gcctgtgggg cgtcatggca ctggtggtgc tcatcacggc
      241 cgcacttgga gaaaccgccc cccgggtcgg gtggtcactg cgactcgaca tcggtggtcg
      301 tggaggatgg ccggagccgg tctgggtggc atctgatctt gaaatgctgg tgagacgcct
      361 ggaacgtgac cgtgaggcga agattgctga agcggaccgt cagggggtgt tttatcgacc
      421 accgccactc ggaacatttc cctcgtggac gcgatgtcag gaactggcgc tgcagcatcg
      481 accggaaatg attctgatcc gggtgaatat ggctggtctc aaagcggcac gccggttgtt
      541 gaagggtggg tatggcgaca gcgacgaaga cagtctgggg cggaatctgc cttcgtggtt
      601 ttcggccgaa cggtggggag aagagattct gcgcgacttt cgtttgtcgg gacgttcgcg
      661 cgagaaaatc tcccggttgc tttctcagca actggacggt gtgaccgctc tctttaccga
      721 gtggcgcgat cgactcaatc acgaggccgc tcaggcaagc cattctctgg agcgggctcg
      781 cctcaaaggc tccgaggacg ctgtgcgtct cgcatgctgg aagctctgga tcaccatggg
      841 ctgcccgtag gcgggataca cgagagtgcc cacgggatcc tgatgcacga acgaacggtc
      901 ctgatccgcc tgttctcgag tcaacccaaa cgccctgttc gtcctgttgt ccttctccga
      961 ttgccctgag attcagtgat tccccatttg cgtaccggag cctgaaaccg agggatttcg
     1021 tttgaatcaa cgtgagtcga gatgacaccc ttttcttgga agattgtact tttcagccaa
     1081 ttgtgatttg ctctttgcat gagcagattc atcgaaactc ttcgcttgtc ccaccttctt
     1141 cctcttattg aacaggagtt ccgcaaggtt cctgaccttc gcgaggctga agcaagtgct
     1201 ttgtgccact ccatgtctga tacattgatg gcaggcttgg ccatgatgtt tgtgcaagac
     1261 ccttcaatgc tggcttttca agcccgtctt caagagaaac aaagagggaa caatcttcgc
     1321 acgatctttg ggatcgagaa agttcccaaa gcttcgcaac tcggtcggat tctggactgt
     1381 gccgatccgc aaacgttgca gagggctttt acgccggttc ttcagtgcct tcagaaaact
     1441 cgcttatggt cgaactttcg ggtgttgggg ggatactatg cggtccttat cgatgggttt
     1501 gagtattttc gtagcgagaa aagaggctgt aagcattgtc aggaatacca ccatcgtgac
     1561 ggtcgagttg agcatgccca tcaagtgctc gcagc
//
LOCUS       DAORHD010001267         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104406_length_1595_cov_3.765584, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001267 DAORHD010000000
VERSION     DAORHD010001267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104406_length_1595_cov_3.765584"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKO06_20740"
     CDS             <1..214
                     /locus_tag="PKO06_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNV72147.1"
                     /translation="EDAHVKARRMLVEMTHPVAGMIKVPGVPIKFSETAAEAVHPAPL
                     LGQHNGEIWGQRLKRQVEQLKKDGVI"
     gene            222..>1595
                     /locus_tag="PKO06_20745"
     CDS             222..>1595
                     /locus_tag="PKO06_20745"
                     /inference="COORDINATES: protein motif:HMM:NF023767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72148.1"
                     /translation="MPHPAIRFLYHGLSRHPGVIIGIIVGAFLLSFGAFTHIEIDDDF
                     EVGITDPQAQAARALYRQHFQHEQAILVALPIAGLTRENLLSLWDLTNELQQLPEVNR
                     VMSILSLLRPCRKREEFERYLRDFRLRNLEELIRRDDLFRGFLVNGDLTALQILVVPD
                     VQVDNYRHKLYKGLNGILSRHAKPGAAHVFGFPYIQERFFEFIVENNQRFLTIGFIGC
                     AILAWFLFPDPVILLLILFAIAAPTVFTFAVYFMNGNKINLFTSPIIPFTLIISLNEI
                     IYIISFFVAERKRDDQTYSLLHEQNFHRLIRPCLINTATTLIGFLSLSQSPSQSIRLF
                     SLYTSLACLFSYLVTFSLIFAFLRLYKPRFSLSSHQRPKFPWLERLQKRLVFRYPIAI
                     LCITAVSIALAVPLLHSYERRNSLSDNFATDDPLLSSHAFIRDRFCGPSHLQVLVSHP
                     NLLQTKQL"
BASE COUNT          324 a          499 c          367 g          405 t
ORIGIN      
        1 cgaagacgcc cacgtgaaag cgcgccgcat gctggtcgag atgacacatc cggttgccgg
       61 tatgatcaag gttccaggtg ttcccatcaa attctctgaa actgctgctg aagccgttca
      121 tcccgcgcca ctcctcggac agcacaacgg ggagatctgg ggtcagcgcc tgaaacgtca
      181 ggtggagcaa ctcaaaaaag acggggtgat ttgagtccgg aatgccgcac ccggcgattc
      241 gcttcctgta ccatggtctt tcgcgacacc caggcgtcat catcggaatc attgtcggcg
      301 cgttcctgct gagtttcggc gcatttaccc acatcgagat cgatgatgac ttcgaggtcg
      361 ggattaccga tccacaggct caggcggcac gagcactcta ccgccagcat ttccaacacg
      421 aacaggcgat tctcgttgct ctcccgattg ccggtctcac ccgtgaaaac ctgctttctc
      481 tctgggatct cacgaatgaa cttcaacaac tgcccgaagt caaccgggtc atgtccatac
      541 tcagtcttct gcgcccttgt cgcaaacgag aggaattcga gcgctatctg cgcgacttcc
      601 gcttgcgcaa cctggaggaa ttgatccggc gcgacgatct gtttcgcgga tttctggtca
      661 acggcgatct cacggcgctg cagatacttg tcgtcccgga tgtacaagtc gacaattacc
      721 gtcacaagct ctacaaaggt ctgaacggta ttctctcgcg gcacgcaaaa ccgggagcag
      781 cgcatgtatt cgggttccca tacatccagg aacgcttctt tgagttcatc gtcgagaaca
      841 accaacgttt tctgactatt ggattcatcg gttgcgccat tctcgcctgg tttctgttcc
      901 cggacccggt cattctcttg ttgattctct tcgcgatcgc cgcgccgacg gtgttcacat
      961 tcgcggtgta tttcatgaac ggaaacaaga tcaacctctt cacgtcacca atcattccct
     1021 ttaccctgat catttctctc aacgagatca tctacatcat cagttttttc gtcgctgaac
     1081 ggaaacgaga tgatcagacc tactccctgc tgcacgagca gaattttcac cgactgattc
     1141 gtccctgtct gatcaacacc gccactacgc tgatcggctt tctctccttg tctcaaagcc
     1201 catctcagag tatccggctc ttctcgctct acacctcact tgcgtgtctg ttctcttacc
     1261 tcgtgacgtt cagcctgatc ttcgcgtttc tccgtttgta caagccgcgt ttcagtctga
     1321 gcagccacca gagaccaaaa tttccctggc tcgaacgcct gcagaagcgc ctggtttttc
     1381 ggtatccgat cgccattctc tgtatcaccg cggtatcgat cgctctggca gtgccgctat
     1441 tgcactccta cgagcgtcgc aactcgctca gcgacaactt cgccaccgat gatccactcc
     1501 tctcctctca tgccttcatt cgcgaccggt tctgcggccc gtctcacctt caggttctgg
     1561 tttcgcaccc gaatctgctg cagacgaagc agctt
//
LOCUS       DAORHD010001268         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104436_length_1595_cov_2.787662, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001268 DAORHD010000000
VERSION     DAORHD010001268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104436_length_1595_cov_2.787662"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKO06_20750"
     CDS             complement(<1..313)
                     /locus_tag="PKO06_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV72149.1"
                     /translation="MPILHVLVLTATLLVALLAPGCGSTPGTAPSAKNVTLTLWNTMG
                     PIETEGLNEVLRLYHEKKPNITVQVENVSFLGGRSRFETAMKAGAGPDIFRADRFWIP
                     PL"
     gene            complement(382..1500)
                     /gene="ugpC"
                     /locus_tag="PKO06_20755"
     CDS             complement(382..1500)
                     /gene="ugpC"
                     /locus_tag="PKO06_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNV72150.1"
                     /translation="MASITLTKVVKQYEGATAATVRGIDLQMRDKEFVVLVGPSGCGK
                     STTLRMIAGLEDITAGEIRIGDVVVNNLPPKDRDIAMVFQNYALYPHMNVRENLSFGL
                     KLRHFEQKEIDKRVEEAARILGLQEYLDRLPKALSGGQRQRVALGRAIVREPKVFLMD
                     EPLSNLDAKLRVQMRAEIKKLHQRLQTTFVYVTHDQVEAMTMADRICLLHNGIIQQYD
                     EPNVIYDRPANVFVAGFIGSPPMNFLKVSRTASGLTLPPANSASPQTWEINLKQHFKN
                     VEGEDNLVLGIRPEHLLLEAKSGTPTLPIPCRVDLTEPMGAETYLYLVTMGGHTLVAR
                     VDPTYRVEVNQSVTLHIPVNKVHLFRDSDTVRIPRTEE"
BASE COUNT          309 a          454 c          487 g          345 t
ORIGIN      
        1 ccagaggtgg tatccagaag cggtccgcgc gaaagatgtc cgggccggct cctgctttca
       61 tggcggtttc gaagcgcgat cgccccccga gaaacgagac attttcgacc tgcaccgtga
      121 tattcggctt cttctcgtgg tagagccgca acacctcgtt gagcccctcg gtctcgatcg
      181 ggcccatggt gttccagagc gtcagcgtga cattcttggc acttggcgcc gtgccaggcg
      241 tgctgccaca cccgggcgcc agcagggcga ccagcagcgt ggccgtcaga accagcacgt
      301 gcagtatggg catggtcgaa ctcctgaccg gtcggcagta ccgtcgcttc ggggcctgcg
      361 gtccgccagc gtgccggccg cttactcctc ggtacgggga atgcgaaccg tatcgctatc
      421 gcggaacaaa tgaaccttgt tgaccggaat gtgcagcgtg accgactggt tcacttcgac
      481 gcgatacgtc ggatcaacgc gggcgaccag cgtgtgacca cccatggtaa cgagatacag
      541 gtaagtttcg gcccccatcg gttcggtcag atcgacgcga cacggaatgg gcagcgtcgg
      601 ggttccagac ttcgcttcga gcagcagatg ttcggggcga atgcccagca cgaggttgtc
      661 ttcgccctcg acattcttga aatgctgttt gagattgatt tcccaggtct gcggcgaagc
      721 ggagttcgcg gggggcagcg tcaacccgga agccgtgcga ctgactttca gaaagttcat
      781 aggcggtgac ccgatgaaac cggcaacgaa gacatttgcc gggcggtcgt agatgacatt
      841 cggctcatcg tattgctgaa tgatgccgtt gtgcagcaga cagatgcggt cggccatggt
      901 catggcttcg acctggtcgt gcgtgacata gacaaaggtc gtctgcaggc gctgatgcag
      961 tttcttgatt tcggcgcgca tctgcacgcg cagcttggca tccaggtttg acagcggttc
     1021 atccatgagg aagaccttgg gttcgcgcac gatcgcgcgg cccagagcca cgcgctgtcg
     1081 ctgaccacca gacaacgcct tgggcagacg atcgagatat tcctgcagcc cgagaatgcg
     1141 cgcggcttcc tcgacacgct tgtcgatctc tttctgctcg aagtggcgca gcttcagacc
     1201 gaacgacagg ttttcgcgca cgttcatatg agggtacaag gcgtaattct gaaaaaccat
     1261 ggcgatatca cgatctttcg ggggcagatt attgaccacg acgtcgccga tgcgaatctc
     1321 gccggccgtg atgtcttcga gaccggcaat catgcgcagc gtcgtgctct tgccacagcc
     1381 cgacggtccg acgaggacga cgaattcctt gtcgcgcatc tgcagatcga tgccgcgaac
     1441 agtggcagcc gtcgcgcctt cgtattgttt gaccaccttg gttaatgtga tggatgccat
     1501 ggttaccctc gtgattgtaa gatttcgctc atgggttcag gttcgttctg cggagtcggt
     1561 tgaaacacgg gcattatgcc cgcgtcgatg aattt
//
LOCUS       DAORHD010001269         1595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104451_length_1595_cov_2.503896, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001269 DAORHD010000000
VERSION     DAORHD010001269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104451_length_1595_cov_2.503896"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..393
                     /locus_tag="PKO06_20760"
     CDS             193..393
                     /locus_tag="PKO06_20760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV72151.1"
                     /translation="MVEQEVPVTSKEVLDITLNAKCSGYDAEFVALATKLQVPLVTCD
                     KKLAREFPKRVFELKDFLKRLS"
     gene            complement(423..980)
                     /locus_tag="PKO06_20765"
     CDS             complement(423..980)
                     /locus_tag="PKO06_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72152.1"
                     /translation="MKASHQARVELFAVLFSVTLLVSPLNSSLFAQDTAATEPSTDLQ
                     NSVSATSPDSSSSASSEMSESDNADMLAQKHPFALENQTKDPFKPLIEKPKPVIVQPD
                     PKPNPTPDPRPQPQPIKPLSLLVQGICGNEGSRWALITYENQVRVVTQDMNVDGTFKV
                     VAIEPDRLIVYSNKHQSRQTFNISK"
BASE COUNT          377 a          355 c          478 g          385 t
ORIGIN      
        1 ctggatcaac gaagccgccc gcgcatttga aacccaggct aaaaaattga cctcaaaaat
       61 gagacccaaa gccgcatact gaacagtctg gttgcacgag aagggcggat gggtctcgat
      121 gttttcaacg agggaatcga cctatgactg cgagagatgg tctattccat tgccttgcat
      181 gcgggagggc aaatggtgga acaagaggtg ccggttactt cgaaagaagt gttagacatc
      241 accttgaatg ccaaatgctc tgggtatgat gccgagtttg tcgctctggc gaccaaactt
      301 caggttccgc tggtcacgtg cgataagaag ctggcacgcg agtttccgaa acgagttttc
      361 gaattgaaag attttctgaa gcggttatcc tgaggatgcg aggggcgaca agatcctgcg
      421 agttacttcg agatgttgaa ggtctggcgg ctttggtgct tgtttgagta gacgatcagg
      481 cggtcgggtt cgatggccac gactttgaac gtaccgtcga cattcatatc ctgggtgacg
      541 acacgcacct gattttcata ggtgatcagt gcccagcgac tgccttcgtt gccgcagatt
      601 ccctgaacca gcaggctgag cggcttgatc ggttgcggct gtgggcgcgg atccggtgtg
      661 gggttcggtt tcgggtcagg ctgcacgatg accggcttgg gtttctcgat caacggcttg
      721 aatggatctt ttgtctggtt ctcgagggcg aagggatgct tctgagcgag catgtcggcg
      781 ttatccgact cggacatttc acttgaagca ctcgaagaac tgtcaggcga agttgctgaa
      841 acgctgttct gcagatcggt cgatggctcg gtggcggcgg tatcctgggc gaagaggctc
      901 gaattcaggg gcgaaaccaa caaggtcacg gaaaacaaca ccgcgaacag ttcaacacga
      961 gcctggtgag aagctttcat aacgtacctc cgtttcgtcg tttgttgtct tttacaacgc
     1021 gtagtggaga aaccggtttg cctgattgtg aaaaaagaaa aactaagacc ggcgagagat
     1081 gcccctgagg tctcctgctg acgatggctg cgggcgtgat tcccgtgcct gacgacccgc
     1141 tcgaatcagc caagcatgcg tggaaatcat gatgcagccg cctgcccgtg accggatgcc
     1201 ttcagcgtct cgaacttcgg gttatgaggg acatcgagtg gctattggca tgtaactatt
     1261 cgtacgagcg tttttcgctt gcaggaattt taaatgtata ctgtcgccga aaataagagt
     1321 ggagaacggg tgccgggatt ttgtcggctg ggtgcttgaa aactcacagt gtgtgatttc
     1381 aggatgagag aacgagatac ttacgattgg gagagggaaa aggagcatct ctcaaacggc
     1441 gactttttgg cgacgctcta atgaacgaga tctcatgcat gtctggagat ctcacaaccg
     1501 aggatataag catacagtct gttctgtatc tagggttaac tgggggctta cgggagggta
     1561 cccctcgttt ggtagaatga aaatgtcaat cgaag
//
LOCUS       DAORHD010001270         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104521_length_1594_cov_3.470435, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001270 DAORHD010000000
VERSION     DAORHD010001270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104521_length_1594_cov_3.470435"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKO06_20770"
     CDS             complement(<1..283)
                     /locus_tag="PKO06_20770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV72153.1"
                     /translation="MANPRIRVLIADDMVELRSNVRRMLSSQEGIMVVGESGDGEETL
                     KACKESQPHVVLMDINMPKLDGLKVTETLSKEHPEIQVVMMSIQSEQEYF"
     gene            complement(328..>1594)
                     /locus_tag="PKO06_20775"
     CDS             complement(328..>1594)
                     /locus_tag="PKO06_20775"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HNV72154.1"
                     /translation="KVFNLLKVSGIIAGPEQKIAEAIKIPTVKVTVIRPLSDTMGIAA
                     QPIDPATGQPVQAQAQFATAPAKLRVVLEGTVKTQADYQHLVEVVRGFVEDEQVSNLV
                     VIEDPIQVTFQAYVLQVNRNNVKDLGVDWGGAQSLSEGLTQGTLRFVENISNAFRGDS
                     QANGAPIERTPNPFAMNNINRFDIIAAQVRAWESKNKVKVLSNPKLTVYANAASKKLA
                     KAGWFDEKDSASNDTEITNDSGLAFVNVGQDVFYISSRDTAGNVSWAKAEAALRLTIR
                     DLYVHENELKFTVFAKQDEPSFSRGTEAPPDILKRSVMTTVKIGNGETVVLGGLIQNS
                     KSVTERGLPVLSKLPYVGRLFRNKSTQTQENELVILLTPEIKGRETNFDRTKKYEVVP
                     VPHRSERLEQLHQMFQQIKGSHFPPETAK"
BASE COUNT          275 a          450 c          497 g          372 t
ORIGIN      
        1 gaaagtactc ctgctcactc tgtatcgaca tcatcacgac ctgtatctcc gggtgttctt
       61 tcgagagagt ttcggtgacc ttcaacccgt cgagcttggg catgttgatg tccatcagca
      121 cgacgtgcgg ctgtgattcc ttgcacgcct tcagggtttc ctcgccgtcg cccgactcgc
      181 ccaccaccat gatgccttcc tgcgaagaca gcatgcggcg aacattcgat cgcagctcga
      241 ccatgtcgtc ggcgatcagg accctgatgc gtgggttagc cacgttgaag aaggctctcc
      301 tccctgttga tttcccgagc ggggtgctta cttcgccgtt tcgggcggaa agtgcgaacc
      361 cttgatctgc tggaacatct gatgcagctg ttccaggcgt tcagagcggt gcggaaccgg
      421 aacgacctcg tacttcttgg tgcggtcgaa gttggtttcg cgacccttga tttcaggggt
      481 caacagaatg accagttcat tttcctgcgt ctgggtcgac ttgttgcgga acaggcgacc
      541 gacgtagggc agcttggaaa gcacgggcag accgcgttcg gtgaccgact tggagttctg
      601 gatcagaccg ccgagaacga cggtttcgcc gttgccgatc ttgaccgtgg tcatgacgct
      661 gcgcttgaga atgtcgggcg gggcttcggt gccgcgagag aagctgggtt catcctgctt
      721 ggcgaagacg gtgaacttga gttcgttttc gtgaacgtac aagtcgcgaa tcgtcaaacg
      781 cagggctgct tcggctttcg cccatgatac gttgccggcg gtgtcgcggg agctgatgta
      841 gaagacatcc tggccgacgt tcacgaaggc cagacccgag tcgttggtga tttccgtgtc
      901 gttcgaggcc gagtctttct cgtcgaacca accggccttg gccagcttct tggaagcggc
      961 gttggcatac acggtgagct tcgggttcga gagcaccttc accttgttct tcgattccca
     1021 cgcacgcacc tgggcggcga tgatgtcgaa gcggttgata ttgttcatgg cgaacgggtt
     1081 cggggtgcgt tcgatcggag caccgttggc ctgtgagtcg ccgcggaacg cattgctgat
     1141 gttctcgacg aagcgtaacg ttccctgcgt cagaccttcg ctgagcgact gtgcgccgcc
     1201 ccagtccacg ccgaggtctt tcacgttgtt gcggttcacc tgcagcacgt aggcctgaaa
     1261 ggtcacctga atcggatctt cgatgaccac gaggttgctg acctgctcgt cttcgacgaa
     1321 gccgcgcacc acttcgacga ggtgctggta gtccgcctgg gtcttcacgg tgccttcgag
     1381 cacgacgcga agcttggcag gagcggtcgc gaactgggcc tgcgcctgca ccggctgacc
     1441 ggtcgcgggg tcgatcggct gggctgcgat gcccatggtg tcagacagcg ggcgaatgac
     1501 cgtgactttg acggtcggaa tcttgatcgc ttccgcgatc ttctgctcgg gcccggcgat
     1561 gatgccggaa accttgagca gattgaagac cttg
//
LOCUS       DAORHD010001271         1594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104523_length_1594_cov_3.439246, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001271 DAORHD010000000
VERSION     DAORHD010001271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104523_length_1594_cov_3.439246"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PKO06_20780"
     CDS             complement(<1..598)
                     /locus_tag="PKO06_20780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72155.1"
                     /translation="MASVRPTGLLLALLIIGTVQGLVSACDKWEHRWRTVQESWQAEF
                     QPEAVAALALVASESLVGVTQVLAPVTEQIHFPEFPVTITPWNSQDPMPDPADLSSIK
                     GSELVTAVTPVLQQAHLLLAVDESLPILSLVKIENLYRTTIRWVKLDRQGTPLTLDET
                     SAAHGVTLTVSVMPRWWPILRVERVLGDPTPERVASHSW"
     gene            complement(598..1551)
                     /locus_tag="PKO06_20785"
     CDS             complement(598..1551)
                     /locus_tag="PKO06_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72156.1"
                     /translation="MTAFLRRHLRTIVVSLALVAALTTLHAGGIASVDYSVLLAFHPQ
                     MARFDFTVQRFFQPDLNLSDSRQMESVRQKMAEKAAQAHTERQNLERERNRIQREIAR
                     IEETSNQTISAMLRDNKDVTSFQNTYRTKLQALNAQLSELDRKFLAIQEAGLDVIYQS
                     REKSQALFLQVQNEIDQHLAAVATERGGVSIIDRSFLAPPASTFAPPVPVSNIDPVTA
                     DLYSQLLNFDFTVPAVEGHGPEGHLDRVKEGVESRFRDQLQRYVSQTPALRPVIANVR
                     GRLLVAGGEDLTALVLGRILDAHRTRPEVKNRLLQLLSSLP"
BASE COUNT          352 a          408 c          511 g          323 t
ORIGIN      
        1 tccacgaatg agaagcgact cgttccgggg tggggtcacc caagacgcgc tcaactcgca
       61 gaatgggcca ccatcgcggc atcacagaaa ctgtcagagt cactccgtgg gcagcgctcg
      121 tttcatcgag agtcagagga gttccctgac gatcgagctt gacccaccga atcgtggttc
      181 gatacaggtt ctcgatcttt accagggaaa ggatcggaag actttcatct acggccagca
      241 gaaggtgggc ctgctgcaga actggggtga ccgccgtgac cagttcggaa cccttgatgg
      301 aggaaagatc cgcagggtcc ggcatcgggt cttgagagtt ccagggggtg atggtgaccg
      361 ggaactcagg gaaatgtatc tgctcagtta cgggcgcgag gacctgggtt actccgacaa
      421 gcgactcaga agccactaac gccagagcag ccacagcttc tggttgaaac tctgcctgcc
      481 agctttcctg aacggttcgc cagcggtgtt cccatttgtc gcaagcgctg accagcccct
      541 gaacggtgcc gatgatcagg agtgcgagaa gaaggccggt gggacgaacc gaagccatca
      601 gggaaggctt gagagcagct gcagcagtcg attcttcact tccgggcgcg tgcgatgtgc
      661 gtcaaggatg cgacccaaga caagcgcggt cagatcttcg ccaccggcga cgagcaggcg
      721 tccgcggaca ttggcaataa cgggacggag cgcgggagtc tggctgacat accgttgcag
      781 ttgatcgcga aagcgcgact cgacaccctc tttgacgcgg tccaggtgcc cttctgggcc
      841 atgcccctca acggctggaa ccgtgaagtc gaaattcaat aactgagagt acaagtcggc
      901 ggttacggga tctatgttag agacggggac tggaggagca aaggttgacg cgggaggggc
      961 gagaaaggat cgatcgatga tggacacccc cccgcgttcg gtggcgacgg cggcgagatg
     1021 ttggtcgatc tcgttctgaa cctgcagaaa gagcgcttgg ctcttctctc gtgactggta
     1081 gatgacgtcg agtccggctt cctgaattgc cagaaatttg cggtccagtt ctgataactg
     1141 ggcgttgagc gcctgcagct tggttcgata cgtgttttga aaagaggtga catccttgtt
     1201 gtcgcgcaac atggcagaga tagtctggtt cgatgtttct tcgatgcggg cgatttcgcg
     1261 ctgaatgcgg ttgcgctcac gttcaagatt ctgccgttcg gtatgagcct gagcggcttt
     1321 ttccgccatc ttctgacgca ctgattccat ctggcgggag tcactgaggt tgaggtcggg
     1381 ctggaaaaat cgctggacgg taaaatcgaa gcgcgccatc tggggatgga acgcaagcaa
     1441 caccgagtaa tcgaccgagg caatgccgcc ggcatgcagg gttgtgaggg cagcgacgag
     1501 cgcgagactg acaacgatag tgcgaaggtg tctacgcagg aaagcggtca tggaatcctc
     1561 ctcttagcgg gtaccgaacg agagattttt cagc
//
LOCUS       DAORHD010001272         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104804_length_1592_cov_3.134027, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001272 DAORHD010000000
VERSION     DAORHD010001272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104804_length_1592_cov_3.134027"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1543)
                     /locus_tag="PKO06_20790"
     CDS             complement(<1..1543)
                     /locus_tag="PKO06_20790"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV72157.1"
                     /translation="MTWNRWRILFFVAFYAAFAAHVALWHYWGQTSVGHLGFGEFFGT
                     LQHGVITAGTIFSLVIFGHALLFGGWFCGWLCHWGMVQDAAIGLMRRFGIHPKMVRLD
                     SRLVPWVWFLILIGQVVLVWLTQGPPATVSWNMSHTPVWSGVPRSILLICITTIISGF
                     FLIYLFGERAFCRTICTFRLWFSWFDRFAPLRIRQTAACTSCAQECTTCCPMGIDVDG
                     EIRAYGEIRDSACIKCNLCIDACPVRSLAPRFSRVMPTAADSSYTPPQPDLQPEAVRL
                     ASLFSLLVLILGAVKIGGNVSMSLGFIIGMVYLRLSAQQRTSIFEISVFAALTLAMIY
                     RVDFHDLTSLGKGLGLIFIFLLLARLIPHPGWQEFVRERAQQRASSLSIAVLAFGIAI
                     LGGRELYNSWNLAQQQASLKSGDYQRLSCMLESFTNFSDNPAATLAELGKTRRRLNDL
                     AGAEKAFRQSLSEKPDPIIFCHLIEVQRLLGSHEEAIQTLEKALGMFAESPDLLLQAA
                     LVALKQ"
BASE COUNT          412 a          421 c          475 g          284 t
ORIGIN      
        1 cctgtttgag ggcgacgaga gcggcctgca gaagaaggtc cggcgactcg gcaaacatgc
       61 ccagggcttt ttccagagtc tgaatggctt cctcatggct gccaagcagt cgctgaacct
      121 cgatcaggtg acagaaaatg atcggatcag gcttttcaga caagctctgg cgaaaggctt
      181 tctccgctcc cgcgagatca ttcaaacgtc tcctggtttt tccgagttcc gccagagtcg
      241 cggccggatt gtcactgaag ttcgtgaacg actccagcat gcaactcagt cgttgatagt
      301 cgcctgattt cagacttgcc tgctgttgtg ccaggttcca ggaattgtac aactcgcgcc
      361 cgccgagaat tgcgatgcca aaagcgagaa cggctattga aagagatgat gcgcgctgct
      421 gagcacgttc gcgaacgaat tcctgccacc cggggtgtgg aataagccgg gccaacaaca
      481 ggaagatgaa gatcagacct aggccctttc ccagagaggt caggtcgtga aagtcaacgc
      541 gatagatcat ggccagggtc agtgccgcga atactgagat ctcgaagatg cttgtccgtt
      601 gctgcgcgct gagacgcaga tagaccatcc cgatgatgaa gcccagagac atactgacat
      661 ttccgccgat tttcacggct ccgagaatca ggaccagaag agaaaacagg ctggccagtc
      721 tgacggcttc gggttggaga tcgggttgcg gaggggtata cgatgaatcg gcggctgtcg
      781 gcatgacgcg cgaaaaccgt ggtgccagac ttctgaccgg acacgcatcg atgcacagat
      841 tgcacttgat gcaggcagaa tcacggattt caccatacgc gcgaatttcg ccgtcgacgt
      901 ctattcccat gggacagcag gtcgtacatt cctgagcgca actggtacag gcggcggtct
      961 ggcgaatacg cagaggtgcg aaacgatcga accagctgaa ccagaggcga aaggtgcaga
     1021 ttgtgcggca gaatgcccgt tcgccgaata gatagatcag gaagaacccg ctgataatcg
     1081 tcgtaatgca gatgagcaga atcgaacggg gaactccgct ccaaacggga gtatgcgaca
     1141 tattccagct gacggtggct ggaggaccct gggtcaacca gaccagaacg acctgcccga
     1201 tgagaatcag aaaccacacc cagggcacca gacgggaatc gagtcgcacc atctttgggt
     1261 gaatgccgaa acgccgcatc agaccgatcg cggcatcctg caccattccc cagtgacata
     1321 gccatccgca gaaccagccg ccaaagagca gggcatggcc gaagatgacg agcgagaaga
     1381 ttgttccagc agtgataacg ccgtgctgca gggtgccgaa gaattcgccg aagccaagat
     1441 ggccgaccga ggtttgaccc cagtaatgcc acagggcgac atgggcggcg aatgccgcgt
     1501 aaaaagcaac gaagaagagg atccgccagc gattccaggt cataactgtc tccgaagggg
     1561 aaagcgggtg agagagtcgg gaagacacga gg
//
LOCUS       DAORHD010001273         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_104832_length_1592_cov_2.569941, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001273 DAORHD010000000
VERSION     DAORHD010001273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104832_length_1592_cov_2.569941"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..779
                     /locus_tag="PKO06_20795"
     CDS             108..779
                     /locus_tag="PKO06_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72158.1"
                     /translation="MKRFTLLFVALCCVSLSSTLAFASEQTLPGNTIFTALSDIVAAP
                     LKFDGRIVSVQATFQGWKNAPGKPPVTRSDWVAQDAAGTAVYCTGAFPEDMRPGDPTA
                     QGRRISILGTVHLDDTGRPFIVVSTALPEAQVETMVSVSQLLFDPVNSRGKLVGLLGV
                     LSKGYGPRGQRMYLLADPTGAITLGRMPKLYPKGTILRLRGIVSADENGLPQITDVEI
                     LSAKP"
     gene            989..>1592
                     /locus_tag="PKO06_20800"
     CDS             989..>1592
                     /locus_tag="PKO06_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72159.1"
                     /translation="MKRRLAQVLCLFVCVGLLLFTGCSGSSGGKPFVYPFAIPTTLTI
                     SGNINLGNVAVHPDLGGITPAIRTPVRASLLDHSPFRIEVEDDPTGTTTVTSSGTCQL
                     PELSIRDQIVVRARNRTHPGFTLEWMGVGAPALLGPTRIEITVPSTARSTIVRTLRNR
                     YGRLMLPEALSDVQILPVAEAMYDVLEAHPEKLSSSVSLAE"
BASE COUNT          295 a          512 c          424 g          361 t
ORIGIN      
        1 attccctcaa ttgaagtcat tcggcgaaaa ggcaaagcgc aggtggatct ccccgagctt
       61 tgccagaaaa ttcgcggaaa atatgcccaa cttggaaagg tggaagcatg aaacggttca
      121 cactgctttt cgtcgccctc tgctgcgtgt ctctctcgtc gacgctcgcg ttcgcttctg
      181 agcaaacctt accgggaaac acgatcttta ccgctctcag cgatattgtc gccgctccac
      241 tcaagttcga cggccgaatc gtctcggttc aggcaacctt tcagggttgg aaaaatgccc
      301 ccggcaagcc ccccgtgacc cgttcagact gggttgctca ggatgccgct ggtaccgccg
      361 tgtactgcac gggcgcgttc cctgaggaca tgcgacccgg agaccccacg gctcaggggc
      421 gtcgcatcag cattctcgga accgttcacc tggacgatac cggccgcccg ttcatcgtcg
      481 tctcaacggc tctgcccgaa gcccaggtcg aaacgatggt ttcggtgtca cagctgctgt
      541 ttgatcccgt caactctcgc ggcaagctgg tcggcttgct cggcgttctc tcgaagggtt
      601 acggccccag gggacagcgg atgtatctcc tcgctgaccc aaccggcgcg atcacccttg
      661 ggcgcatgcc gaagctgtat ccgaagggca ctattctgcg tctgcgcggc attgtctcgg
      721 ccgacgaaaa cggcttgccg cagattaccg acgtcgaaat tctctccgct aaaccctgat
      781 tacgtccgag ttcacaagtg ggtgcatctt gcatgcgccc acttttcctg taccgacatt
      841 cagaatctga tcgtccaaag gaatcgcgac cgtgatgtgt ctgccactga agagccgtgt
      901 ttgttcacac ctgtctcact ccttgcatca gcattcagtt atctgcctgc cgggacattc
      961 acgtttctac ccgacgatgc cattggtcgt gaagcgtcga ctcgcacagg ttctttgcct
     1021 ctttgtctgt gtcggtctgc tgctgttcac cggttgttca ggctcttccg gtggaaaacc
     1081 atttgtctac ccgttcgcta ttccaaccac gctcactatt tcggggaaca tcaacctcgg
     1141 caatgtggcc gtgcaccccg atctcggggg catcaccccg gcgatccgca ccccggtgcg
     1201 tgcatcgttg ctcgaccata gtcccttccg catcgaagtc gaagacgatc cgacaggaac
     1261 gaccaccgtc acctcgagcg gcacatgtca gcttccggag ttgtcgattc gcgatcagat
     1321 cgtggtgcgc gcccgcaacc gcacgcatcc agggttcacg ctggagtgga tgggggttgg
     1381 cgcgccggcg ctgctcggcc caacacgtat cgagatcacc gtgccttcaa cggcccgttc
     1441 gaccatcgta cgcacgttgc gcaaccggta cggacgcctc atgcttcccg aggctttatc
     1501 cgacgtccag attctcccgg tcgcggaagc catgtatgac gtgctcgaag cccacccgga
     1561 gaagctctca tcgtcggttt ctcttgccga gg
//
LOCUS       DAORHD010001274         1588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105219_length_1588_cov_3.537508, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001274 DAORHD010000000
VERSION     DAORHD010001274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105219_length_1588_cov_3.537508"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PKO06_20805"
     CDS             <1..658
                     /locus_tag="PKO06_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72160.1"
                     /translation="KFSNGDDLTASENGTPVAPRADDLDEYLTDPQNVQAVNQDKPLA
                     NAEPLPMDAYRDRATHVIEGDLTLSGQVEIWGLWYVTGRVTILDCAISGMGAIVAQNG
                     FLAQGHLVHQNPGHDFLSLITDQTLSVAPLKNEFVGLHAAVYADRGVSGKPTDKVKIY
                     GNLVTRALPLPDHRPRIAVVFDTTIVNKPRHALAWFLPWNKVIAVLSERHFNRRVYAR
                     "
     gene            648..>1588
                     /locus_tag="PKO06_20810"
     CDS             648..>1588
                     /locus_tag="PKO06_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72161.1"
                     /translation="MLDNHPPSPPEARPGSSRLLQAAAVLFLIWCVVSLLLSLRPGGH
                     DLFRLVPLDMTAVEHAAGVTGVASSAPINSVFDAQGFPVSASPDPVVADDQLAGRQQI
                     LAYLQAKAGAGRPDEILKSFEGKFGRYWKDPRREGFLEESRARSLKLNEMANRNRQLV
                     QSVNRFLQSDAGKAVSAALKGSLSDAIESPARNTLHAAFSAWKNGDLAQAYQLLKQAE
                     SLDSRSAFIQIMAHQAMAFLNQERHGPLEETLRQYRDLADETHQYLSRFRPVLAKAGF
                     DPVKLYGRDPFDARGLPTSEQFEKAMNQSRYVQFERA"
BASE COUNT          329 a          493 c          448 g          318 t
ORIGIN      
        1 caagttcagc aacggtgacg atctcacggc ttccgaaaac ggaacaccgg ttgccccccg
       61 cgctgacgat ctcgacgagt atctgaccga cccgcagaat gtccaggccg tgaatcaaga
      121 caagccgctg gccaacgccg aaccactccc catggatgcc tatcgcgatc gagccacgca
      181 tgtcatcgag ggcgatctca cgctttccgg acaggtcgag atctggggtt tgtggtatgt
      241 caccgggcgc gtgaccattc tcgactgcgc gatctcaggc atgggcgcga tcgtcgctca
      301 gaatggattt cttgcccaag gccatctggt acatcagaat cccgggcacg attttctctc
      361 gctgatcacc gaccagacgc tgtccgtcgc tcccctgaag aacgagttcg tcgggctgca
      421 cgcggctgtc tatgccgatc gtggagtttc cggaaagccc accgacaagg tcaaaatcta
      481 cgggaacctg gtcacacggg cgctgccgct tcctgatcat cggccacgca tcgctgtcgt
      541 cttcgatacc acgatcgtga acaaaccccg gcatgctctc gcctggtttc tgccctggaa
      601 caaagtcatc gcggttctca gcgaacgtca cttcaatcgg agagtctatg ctcgataatc
      661 accccccctc tccgccggaa gcccgaccgg gttccagtcg actgctgcaa gccgccgccg
      721 tgctcttcct catctggtgt gtggtgtcgt tgcttctcag tctgcgaccg ggtggtcacg
      781 acctgttccg tctggtaccg ctcgacatga ccgcggtcga acatgcagca ggggtcacgg
      841 gtgtagcctc gagtgccccg atcaactctg tgttcgatgc gcaggggttt ccggtcagcg
      901 caagccccga ccccgtcgtc gcagacgacc agctcgcggg ccgccagcaa attctcgcgt
      961 atctgcaggc aaaagccggg gcaggtcgcc cggacgagat actgaagagc ttcgagggca
     1021 agttcggtcg ctactggaag gatccccgcc gggaaggatt tctcgaagag tcacgagccc
     1081 ggagcctgaa gctcaacgag atggcaaacc ggaatcgaca gctggtacaa tcggtgaacc
     1141 gatttctgca gagcgacgcc ggaaaagcgg tctccgcggc actgaaagga agcttgtcag
     1201 atgccattga atctccggca cgcaacactc tgcatgcggc tttttcggca tggaaaaacg
     1261 gagatctcgc tcaagcgtat cagcttctga aacaggccga gagcctcgat tcccgttccg
     1321 cctttattca aatcatggcg catcaggcca tggcgtttct gaaccaggag cgccatggac
     1381 cactcgagga aacgctgcgg cagtatcgtg atcttgccga cgaaacacat cagtatctct
     1441 cgcgctttcg ccccgtcttg gcgaaggccg gtttcgaccc ggtgaagctg tatgggcgcg
     1501 atccttttga tgcccgcggt ctgccgacca gtgaacagtt tgaaaaggcc atgaaccaga
     1561 gccgatacgt gcaattcgag cgggccta
//
LOCUS       DAORHD010001275         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105366_length_1587_cov_3.012402, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001275 DAORHD010000000
VERSION     DAORHD010001275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105366_length_1587_cov_3.012402"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKO06_20815"
     CDS             complement(<1..797)
                     /locus_tag="PKO06_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72162.1"
                     /translation="MELAYRLILYILIANTGFMLYSLARRGWRANAGYLIQLGLILVF
                     MIVMLIWGTSGFWAVIVSAGCIIVSVVIPFFLQRRLETLLAEQRFDEIENVAWWKAAL
                     AWSEVNSHLLAIGRIAANMNESPAAVESELRHLLGKGEPFDGITRLFMAMLHFHQRRF
                     AEIVDDLASPDRREEDLSFEELLYLARGYLETRRFADACRIQRQLEQKAAHETSPHTA
                     GNLLVSRLIFFALMGWKEEFHRLLEQHPDLRASLPEILREFWNGVTV"
     gene            complement(919..>1587)
                     /locus_tag="PKO06_20820"
     CDS             complement(919..>1587)
                     /locus_tag="PKO06_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72163.1"
                     /translation="TTSASAHVVASSGEFSMQQTEGQIELSFQLEHSETRFDMRQVHL
                     QGEELTRSVQKIDPLILDLDGDGFDLTQAGSGGSFDIDADGTLDQTAWVRGDDALLFV
                     DRNGNERPDNGKELLGDQNGATNGFAELARYDDNHDRVINKHDSVFRALRLYRDLNGD
                     GQVQLGESTTAEQAGVSAINLEFFRDGRTINGNDLLLRGSFTTSDGKTNTIADFLLGY
                     RSGA"
BASE COUNT          353 a          448 c          451 g          335 t
ORIGIN      
        1 tagacagtca ccccgttcca gaattcccgg agaatctcag gcagggaggc tcgaagatcg
       61 ggatgctgtt cgagcaggcg gtgaaattct tccttccatc ccatcagggc gaaaaagatc
      121 agacgactga ccagaaggtt cccggcggtg tgaggagaag tctcatgcgc tgctttctgc
      181 tcaagttgtc gctgaatgcg gcaagcgtct gcgaaacgcc gggtttcgag gtagccgcgt
      241 gcaagataga gcagttcttc gaagctgagg tcttcttcac ggcggtcggg tgaagcaaga
      301 tcgtcgacaa tctcggcaaa acgccgctga tgaaaatgga gcatagccat gaacagccgc
      361 gtgatgccat cgaaaggctc tccctttccg agcagatgtc gaagttctga ttcaaccgcg
      421 gccgggcttt cgttcatgtt ggcagcgatt ctgccgatgg ccagcagatg cgaattgact
      481 tcgctccagg cgagggccgc cttccaccag gccacgtttt cgatttcgtc gaagcgttgc
      541 tcagcaagaa gagtctcgag gcgtcgctgc agaaaaaagg gaatgacgac cgacacgatg
      601 atgcatcctg ccgatacgat cacggcccag aacccactgg taccccagat cagcatcacg
      661 atcatgaaca ccagaatcag gccgagctga atcagatagc cggcattggc ccgccatcca
      721 cgacgggcta acgaatacag catgaatccg gtattcgcga tgaggatgta gagtatcagc
      781 cggtaggcca gttccatcgc cacgaagtat acacgagttt tcctgtggcg acgatgaaaa
      841 ccacgagggt ttccgtggca tcatccgacc acgcactccg gtgggtacct tctttcgtca
      901 tcccccagtc cggtccgatc aggctccgga acggtagccc agcagaaagt cggcgatggt
      961 gttggtcttg ccatcgctcg tggtgaacga accgcgcagt aagagatcgt ttccgttgat
     1021 cgtacgaccg tcgcggaaga actccaggtt gatggcgctg actcccgcct gctcggcggt
     1081 cgtcgactct cccaattgta cctgtccgtc accatttaaa tctcgatata gacgcaatgc
     1141 gcgaaagacg gaatcgtgtt tgttgattac tcggtcgtga ttgtcatcat accgtgccag
     1201 ttcggcaaag ccgttcgtgg ccccattctg atcgccgaga agttccttgc cgttgtcagg
     1261 acgctcattg ccgttgcggt cgacaaagag cagggcatcg tcaccccgca cccacgcggt
     1321 ctgatccaga gttccgtcgg cgtcaatgtc gaacgaaccg cctgaccctg cctgcgtgag
     1381 atcgaagccg tctccgtcga ggtcgagaat cagcgggtca atcttctgaa ccgaacgggt
     1441 gagctcctcg ccttgcagat gaacctgtcg catgtcgaag cgggtttcag aatgctcgag
     1501 ctgaaacgag agttcgatct gcccttcggt ctgctgcatc gagaattcac ccgatgaggc
     1561 cacgacatga gcgctggccg aggtggt
//
LOCUS       DAORHD010001276         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105392_length_1587_cov_2.595300, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001276 DAORHD010000000
VERSION     DAORHD010001276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105392_length_1587_cov_2.595300"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1587
                     /locus_tag="PKO06_20825"
     CDS             <1..>1587
                     /locus_tag="PKO06_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72164.1"
                     /translation="RRRFPGMLSVVVVDANGELIDALTDGVISRTVARRLFYTLLREK
                     RGELKPEEAKSAWPVLQSFVGPHAVMTDIISSRPSLRLASHADRKTFFFPALDEGGAL
                     FAHVNRSSDWRLLALKDQLRRSRSRRRTSDLEVGLIESGSTASGPQELRAAFDSFEVN
                     LQTHQKVDDRWYAFNRLSDGSTLWASLPDPFMERYHLLRLRLALLVVIGFSLYLVQAA
                     RWTFGPDPLTLSIRRRLILLFGFCAGLPLCVLWITGWDYLDHLERVRTRETYDEVERQ
                     LWAFDTRFRQMRHHLERRLSKAMEQCSFRTPAEREASFDIIRTLAAEFETTDIMLFDH
                     RGKLQWRYPERFHLQKEAASKLLGEVTGRLCNILNGDEEGGKNVATGLLLDTLQEFGG
                     GLDIIKQLASTLGTITEFSSGAEKAWVYLARFADRPGHIGHMVMFNWRKTDLEKYYLE
                     RSILSAEREWQGLRLLAYNAQGPVRSFTPSFHPFIQPALPVMLEQRQRETTVFRPIAV
                     DGNTWLACAMKPRELGDYYL"
BASE COUNT          311 a          459 c          455 g          362 t
ORIGIN      
        1 gcgtcgtcgc tttcccggca tgctgagcgt ggttgtggtg gatgccaatg gcgaacttat
       61 cgatgcactc accgacggcg tgatctcacg gacggtggcc cggcggttgt tttacacgct
      121 cctgcgtgaa aaacgtggcg agttgaagcc cgaagaggcg aaatccgcct ggccggtgtt
      181 acagagtttt gttgggccac atgctgtgat gaccgacatc atttcgagtc gcccgtcact
      241 acgactggcc agccatgccg atcgcaagac cttcttcttt cctgccctcg acgagggtgg
      301 cgcgctgttc gcgcacgtga atcgttcgtc tgactggcga ttactcgccc tcaaagatca
      361 gctgcgccgc tcgcgcagcc ggagacgaac gtccgatctt gaagttggtc tgatcgaatc
      421 gggatcgacc gcttcgggtc cgcaagagtt gcgtgctgcg tttgattcct tcgaagtcaa
      481 tctgcagaca catcagaaag tcgacgaccg atggtacgcg ttcaaccgcc tgtctgatgg
      541 ctcaaccctc tgggcgtcgc ttcccgaccc cttcatggaa cggtatcatc tgctccgttt
      601 gagactggcg ctgctggtgg tcatcggatt ctctctgtat cttgttcagg cagcacgctg
      661 gacctttgga cctgacccgc tgaccctgtc gatacgccgg cgactgattc tcttgtttgg
      721 cttttgtgcc ggtcttccgc tgtgtgttct ctggatcacc ggctgggact atctggacca
      781 tctcgagcgt gtacgcaccc gtgaaaccta cgacgaggtc gagcggcagc tctgggcgtt
      841 tgatacccga tttcgccaga tgcgccatca cctcgaacgc cggctgtcaa aggccatgga
      901 gcagtgctca tttcgtactc ccgccgaacg tgaggcgagt tttgacatca tcagaacgtt
      961 agcggccgag tttgaaacga ccgacatcat gctctttgac catcgcggca agctgcagtg
     1021 gcgatacccc gagcgcttcc atctgcagaa ggaagctgcc tcaaagctgc tcggagaagt
     1081 gacggggcga ctctgcaaca ttctgaatgg cgacgaagag ggcggaaaga acgtcgcgac
     1141 tggcctgttg ctcgacaccc tgcaggagtt cggcggcgga ctcgacatta tcaaacaact
     1201 ggcgagcacg ctcggaacca tcaccgagtt ttcctcaggc gctgaaaaag cctgggtgta
     1261 tctcgctcga tttgctgatc gcccgggtca catcgggcac atggtcatgt tcaactggcg
     1321 caaaaccgac ctcgaaaagt actacctcga acgctcgatt ctgagtgctg aaagagaatg
     1381 gcagggtctg cgactgctgg cttacaatgc ccagggtcct gtgcgaagct tcactccgtc
     1441 atttcatccg ttcattcagc cggcgttacc ggtaatgctc gaacagcgac agcgtgagac
     1501 caccgtgttt cgcccgatag cggtagatgg caacacctgg ctggcatgtg ccatgaaacc
     1561 gcgcgagctg ggcgattact atctgtg
//
LOCUS       DAORHD010001277         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105474_length_1586_cov_3.252776, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001277 DAORHD010000000
VERSION     DAORHD010001277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105474_length_1586_cov_3.252776"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..726)
                     /locus_tag="PKO06_20830"
     CDS             complement(46..726)
                     /locus_tag="PKO06_20830"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV72165.1"
                     /translation="MIDLKTFSFFNTLPTRETEKIKKICRIQKYISGTTILDAGEELK
                     EFILVIEGQVSVLKVHNGKKKTLYSLDPGDTYGEVEILNGTSSLATLIGYQDFQLMFI
                     PKDAVLRLIGLYPGFAKEIRDNFFRRAQILLEQGSSKTPFGQVITFFNVKGGAGKSVM
                     SANTAVMLTTQWKKRVVLIDGNLSFGDQAILLSLPQDKNSYELSKIRPPIGIEQIESQ
                     LTTHPSGL"
     gene            924..>1586
                     /locus_tag="PKO06_20835"
     CDS             924..>1586
                     /locus_tag="PKO06_20835"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV72166.1"
                     /translation="MSTRMKVLFVCTVVLVFGALSPALAGNPYGDENVSGVEGTNVAQ
                     GGATAQAPVTAVQIPDQFTGTVFTGDANLNVRSGPWGDIIGSLSNGTKIKVVGKHFDW
                     YKIEHNGKTAYVHSAWVLAPGETAKPFPRAGWVNAPTGLNVRRVPNGDVIGTLKDQVH
                     VEILGVAGDFYKIKYGNNEAFVSRKYIDTNQPGKPSGEQVTAANFTGYVTASSLNVRE
                     APW"
BASE COUNT          369 a          418 c          446 g          353 t
ORIGIN      
        1 actcgatccg gccgcccatc ggcatcgaac agatcgagtc acagctcaca gcccggacgg
       61 atgggtcgtg agctgtgact cgatctgttc gatgccgatg ggcggccgga tcttcgacaa
      121 ctcataggaa ttcttgtcct gagggagtga cagcagaatc gcctgatcac cgaacgacag
      181 attgccgtcg atcaacacga ccctcttttt ccattgcgtg gtcagcatga ccgccgtatt
      241 ggctgacatg accgacttgc cggctcctcc cttgacgttg aaaaaggtga tgacctggcc
      301 aaagggggtt ttgctggaac cctgctcgag gagaatctga gctcgtctga agaaattgtc
      361 gcgaatttct ttggcaaacc ccgggtacag gccgatcagc cgcagcacgg cgtctttggg
      421 aatgaacatc agctgaaagt cttgatagcc gatgagcgtg gccagagaag aggttccgtt
      481 gaggatctcg acttcaccgt aggtgtcacc gggatccagc gagtacaggg tcttcttctt
      541 cccgttatgg actttcagaa ccgagacctg cccctcgatg acgagaatga actctttcag
      601 ctcttcacct gcgtcgagaa tagtcgtacc cgagatgtac ttctgaatcc ggcagatctt
      661 tttgatcttc tcggtctcgc gcgtgggcag cgtattgaaa aaggaaaatg tttttaaatc
      721 aatcatggtg tttcccctcg tgaataagaa cgtgcaaata tactccgtgc cggtggaaga
      781 aacaactgtc acgttcggag cgtttcgcac gtttactgaa ccgacggaaa gattctcttc
      841 catagggaaa agattcaggg gccagtaaac ttttcgatcg gaattctgta ttatatggta
      901 atcccgtaga aggagcaggg cgtatgtcta ctcgaatgaa agttctcttc gtatgcaccg
      961 tggtgctggt tttcggggca ttgtcaccgg ctctggctgg taatccctat ggcgacgaaa
     1021 atgtcagcgg cgttgaagga acgaatgtcg cgcagggagg cgccacggct caggctccgg
     1081 tgaccgcggt gcagatcccg gatcagttca cgggaacggt ctttacgggc gatgcgaacc
     1141 tgaacgttcg ttccggccca tggggagaca tcatcggatc actttccaac ggcacgaaaa
     1201 tcaaggttgt tggcaagcac tttgactggt acaaaatcga acacaacggc aagaccgcct
     1261 atgtgcattc cgcctgggta ctggctcccg gagaaaccgc caagcctttt ccccgcgctg
     1321 gctgggtgaa cgcgccgacg gggctgaatg tgcgccgcgt gccgaacggc gatgtcatcg
     1381 gaacgctcaa agaccaggtg cacgtcgaga ttctgggggt tgccggcgac ttctacaaga
     1441 tcaaatacgg caacaacgaa gcgtttgtca gccgcaaata catcgatacc aaccaacccg
     1501 gaaagccttc gggcgaacag gtgacggcgg ccaactttac cgggtatgtc acggccagct
     1561 cgctgaatgt tcgcgaagcc ccatgg
//
LOCUS       DAORHD010001278         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105703_length_1584_cov_2.986920, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001278 DAORHD010000000
VERSION     DAORHD010001278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105703_length_1584_cov_2.986920"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="PKO06_20840"
     CDS             <1..777
                     /locus_tag="PKO06_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HNV72167.1"
                     /translation="KAMLEDIAILTNGKKISEEIGMKLDKTTVEHLGQAKKVVVSKEK
                     TTIIEGAGKPKDIQARIHTIKAEIDQSTSSYDKEKLQERLAKLSGGVAVIKVGAATET
                     EMKERKTRVEDALSATRAAVEEGVVAGGGVTYINALPVLDGVKWENEDEKIGIDIIRK
                     ALYAPMRFILSNAGLEASVIIEKVRENKKANQGYDVAKNEFADMFKNGVIDPAKVTRS
                     ALQNASSIASILLTTEVLVAEVPEKKNNAPAGNPGMDMDY"
     gene            874..>1584
                     /locus_tag="PKO06_20845"
     CDS             874..>1584
                     /locus_tag="PKO06_20845"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72168.1"
                     /translation="MTDQTIRIQAAVGGESFPSLNGRQKQVLKEVCESFISTGIPVGS
                     RTISRFGGLACSPATIRNEMADLESLGYLFSPHTSAGRVPTEMGFRFYVNSLIEYGRL
                     CQIEEKLVTRLIEKFEAERAHQKAVLQSAVRFASEVTHLTGVLLTPQKASRKLRSIQV
                     VRLFEDQVLLVTVDDCGRISEQIFGIPANTSDESLRELTRLLNTEICNRELFDTQNRV
                     LREMQQILSQHNQLLQTLL"
BASE COUNT          376 a          444 c          445 g          319 t
ORIGIN      
        1 aaggccatgc tcgaagatat cgccattctg accaacggga agaagatcag tgaagagatc
       61 ggcatgaagc tcgacaagac caccgtcgag cacctcggcc aggccaagaa ggtcgtcgtt
      121 tcgaaagaaa agaccaccat catcgaaggc gcgggcaagc cgaaagacat tcaggctcgc
      181 attcacacca tcaaggctga aatcgaccag agcaccagca gctacgacaa agagaagctt
      241 caggaacgcc tggcgaagct ttccggcggc gtggcggtga tcaaggtcgg agccgcgacc
      301 gagaccgaaa tgaaagaacg caagacccgt gtcgaagatg ctctttccgc gacccgcgcg
      361 gccgttgaag aaggcgtcgt cgccggcggc ggcgtgacct atatcaatgc tttgcccgtt
      421 ctggacggcg tgaagtggga aaatgaagat gaaaagatcg gcatcgacat tatccgcaag
      481 gctctgtatg ctccgatgcg cttcatcctc agcaacgccg gtctcgaagc ctccgtcatc
      541 atcgagaagg tgcgcgagaa caagaaggcg aaccagggct acgatgtggc caagaacgaa
      601 ttcgccgaca tgttcaagaa cggcgtcatc gatccggcca aggtcacccg ctcggctctg
      661 cagaacgctt cctcgatcgc ttcgattcta ctgaccaccg aagtgctggt cgccgaagtg
      721 cccgagaaga agaacaacgc tccggccggc aaccccggca tggacatgga ctactgagtg
      781 aaccctgact gaccgtcgac ggctctccgg tttcgaccgg agagccgttt tcgacgctca
      841 atccttgact tggtttctta acgagtttta cccatgacag accagaccat tcgcattcag
      901 gccgctgtgg gcggagagag ttttccatcc ctgaatggtc gacagaagca ggttctgaaa
      961 gaagtctgcg aatcgtttat atccaccggc attccggtgg gatcacgcac catatcacgg
     1021 tttggtggac ttgcttgttc gcccgcgacg attcgcaacg aaatggccga cctcgagtcg
     1081 ctgggctatc ttttctcgcc gcatacctcg gcggggcggg tccccacgga gatgggattt
     1141 cgtttctacg tcaactcgct gatcgagtat ggtcggctgt gccagatcga ggagaagctg
     1201 gtcacacgcc tcatcgaaaa attcgaagcc gagcgggcgc atcaaaaggc ggttttgcag
     1261 tcggccgttc gctttgcgtc tgaagtgacg catcttaccg gggtgttgtt gaccccgcag
     1321 aaagcttcac gcaaattgcg atccatccag gtggttcgcc tgttcgaaga tcaggtgctg
     1381 ctggtcaccg tcgatgattg cggtcggatt tccgaacaga ttttcggcat tcctgccaac
     1441 acttcggacg aatcattgcg tgaattgacc cgcctgctga ataccgaaat ctgcaatcga
     1501 gaattgtttg acacgcaaaa ccgcgtcctg cgtgagatgc agcagattct gtcgcagcac
     1561 aatcagctgc tgcagacgct cctg
//
LOCUS       DAORHD010001279         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105742_length_1584_cov_2.159581, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001279 DAORHD010000000
VERSION     DAORHD010001279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105742_length_1584_cov_2.159581"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="PKO06_20850"
     CDS             complement(<1..1548)
                     /locus_tag="PKO06_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72169.1"
                     /translation="MTNGTDWLDVAQLTMTPAVINGFPRAAIVYPSITLDVPVAQPYG
                     LYQGLLIAYGDIDNDGIRDSGEPWCYMTLRVEVGDKQVTIVAPAAVNLSGDPATFTNV
                     VSVSAKNTGYLTLTKVKAIGTDFAGPDTLASDVFLFAPSNNLGYLLLNQTKSFNVWCS
                     LPWGQTDGVYTGSLWVWDDSNNDGVIQSAEASNSVPISLAVGLKKVLNTTPPTLDLGI
                     WTRGDVASAGFLAQNVGNVALIDARWLLAALDGGGPTIPTGNISITPAVAGAMAKPPI
                     GLPVSVSSTCTVTIPAGTPDGLYQADMTFFEDEITPAANNYDVGEPFWSMNVRITVAT
                     PFVTAVPPNLNWSSDPGMQTGTQTMTISNTGPMSLNRLRWSFTNLVSGGNTIASSAIS
                     FFPTTAPILAPGASFVSSFSVLIASPTQAPGVYTGTFLVYEDRNNNATFDATEANDTV
                     ALSLTVNAVQKVDVAPVLVDFGKIARGTASSWLTFQLQNTGNVALNGMGWIEVPLLGA
                     SGTIPAAS"
BASE COUNT          361 a          455 c          451 g          317 t
ORIGIN      
        1 gctcgcggcc ggaatggtgc cgctggctcc cagaagcggt acttcgatcc agcccattcc
       61 gttgagcgcc acgttgcccg tgttctgcaa ctggaatgtt aaccacgaag acgcggtgcc
      121 acgcgcaatc ttgccaaaat caaccaggac aggagccacg tctaccttct gaacggcatt
      181 cacggtcaga gagagagcca cggtgtcgtt ggcctcagtc gcatcaaatg tggcgttgtt
      241 gtttcgatct tcgtagacca gaaatgtccc ggtgtagacg cctggtgcct gggtcggcga
      301 cgcgatgagt accgagaacg atgagacaaa tgaagctcca ggagccaata tcggggcggt
      361 tgtcgggaag aaactgatgg cacttgaggc gatggtatta cccccgctga cgaggttggt
      421 aaacgaccag cggagtcgat tcaggctcat gggcccggtg ttactgatcg tcatcgtctg
      481 cgtcccagtt tgcatacccg gatcactgga ccagttcagg ttcggcggta cagcggttac
      541 aaacggggtg gccaccgtga tgcgaacgtt catggaccag aatggttccc cgacatcgta
      601 gttgttggcc gcaggggtga tctcatcttc aaagaacgtc atatcagcct ggtagaggcc
      661 atcaggtgtc ccagcgggaa tcgtcaccgt acaggtcgac gacacgctca cgggcaaccc
      721 aataggaggc ttggccatgg cacccgcaac cgccggggta atgctgatgt ttcctgttgg
      781 aatcgtcggg ccgccaccgt cgagagccgc cagcagccag cgcgcatcga tgagagcgac
      841 attgccgaca ttctgtgcca gaaatcccgc actcgcgaca tcgccgcggg tccagatgcc
      901 aagatcgaga gtcggtggcg tggtattcaa caccttcttc aagccaaccg ccagcgagat
      961 cggcacgctg tttgaggcct ctgccgactg aatcacccca tcgttgttgc tgtcatccca
     1021 cacccatagc gatccggtat acacgccatc ggtctggccc cagggcagcg agcaccagac
     1081 gttgaaactc ttggtctgat tcagcagcag atatcccaga ttgttggaag gagcgaaaag
     1141 aaacacatct gaagccagcg tatctggacc ggcgaaatcc gtgccgatag ccttcacctt
     1201 ggtcagagtc agatacccgg tattcttggc actcaccgag accacgttgg taaaagtggc
     1261 tggatcgccc gaaagattca ccgccgcggg tgccacaatg gtcacctgtt tgtcacccac
     1321 ctcaacccgc aacgtcatgt aacaccaggg ttcaccgctg tcgcgtatcc catcgttatc
     1381 gatatcgccg taagcgatga gaagaccttg atacaagcca tacggctgtg ccaccggaac
     1441 atcgagggtg atggacggat agacaatggc agctctcggg aaaccattga tgaccgctgg
     1501 tgtcatcgtc aactgcgcga cgtcgagcca atccgtaccg ttggtcattg aagcggtctc
     1561 gaagcgaacc cacggaatat cgag
//
LOCUS       DAORHD010001280         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105809_length_1583_cov_3.594241, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001280 DAORHD010000000
VERSION     DAORHD010001280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105809_length_1583_cov_3.594241"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="PKO06_20855"
     CDS             <1..776
                     /locus_tag="PKO06_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72170.1"
                     /translation="WLRHLCRRGWIIIFPQYQGSGEKPDYFTTNAALGIRKAMRYLND
                     GGGVTPDRDRCATIGHECGAVVGANLAAAARYYKIPVPQAIMNLMPSRGTGLEFYDLS
                     TIPGGTLYLVVVGEDDHANGVTTARQMFYAADSVLTRDKSFITLLSDIHGTPALMADR
                     FAPLAPLEPPYEHEIDRRRWEFLHAAFHKGPHTRPVRGRGIDAMDYNGTWRLFDAMCQ
                     VIWNQGDRQEVFDNTEQQRNMGFWSDGIPIRGLLSSDRP"
     gene            833..>1583
                     /locus_tag="PKO06_20860"
     CDS             833..>1583
                     /locus_tag="PKO06_20860"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV72171.1"
                     /translation="MSAWQAVDSWLDAAVNRMVEFQRELVAIPAIAPESGGDGEYLKA
                     RHLELLLQKMCWKPILDFPAPDDRVSHRQRPNLVYRRPGAQHDYTVWIMAHTDVVPEG
                     DRTKWQSEPFTMVQKQDLLIGRGVEDNHQGLTAGVFALQALSECAVVSNHDVGMIFVA
                     DEECGSQYGIQYLLRAHPDLFGPRDVVLVPDSGNSDGTMIEVAEKSILWLKFTTTGKQ
                     CHASRPYNGKNAFAAAAELVVSLGRLPEVWPQ"
BASE COUNT          325 a          498 c          448 g          312 t
ORIGIN      
        1 ggtggttgcg ccatctctgc cgccgcggct ggatcatcat ctttccacag tatcagggaa
       61 gcggcgagaa gcccgactac ttcaccacga acgccgccct cggcattcgt aaagccatgc
      121 gctacctcaa cgatggcggc ggagtaacgc ccgatcgcga ccgttgcgca acgattggtc
      181 atgagtgcgg tgcggtcgtc ggcgccaatc ttgccgccgc agcccgttac tacaaaatcc
      241 ccgttcccca ggccatcatg aacctcatgc cgtctcgcgg caccgggctc gagttttacg
      301 atctctcaac gattccgggg ggtaccctct atctcgtcgt cgtcggtgaa gatgaccatg
      361 ccaacggcgt caccacggcc cggcagatgt tttacgccgc tgattcggtg ctgacacgcg
      421 acaagagctt catcacgctg ctctcagaca ttcacggcac gccggcactc atggccgacc
      481 gctttgcccc attggctccc ctcgagcccc cctacgaaca tgagatcgat cggcgccgtt
      541 gggagtttct gcacgccgca tttcacaaag gtccgcacac acgcccagtc cgaggtcgcg
      601 gaatcgacgc catggattac aacggcacct ggcggctgtt cgatgccatg tgccaggtca
      661 tctggaacca gggggatcgt caggaagtct tcgacaacac ggagcagcag cgaaacatgg
      721 gattctggag tgacggaata ccgattcgcg ggctgctctc ttctgatcgt ccctgacgcg
      781 tgaacgggct tctccgtatt acacggtttg agacaccgac aaggagactg caatgagtgc
      841 ctggcaagcc gtcgattcct ggctggacgc tgctgtcaat cgcatggtcg agtttcagcg
      901 cgaactggtc gccattcccg ccatcgcccc ggaaagcggc ggggacggag agtatctgaa
      961 agcccgtcac ctggaactgc tcctgcagaa aatgtgttgg aagccgattc tggactttcc
     1021 tgccccggac gatcgcgtat cgcaccgcca acgtccgaat ctcgtctatc ggcgcccggg
     1081 ggcacagcac gattacacag tctggatcat ggcccacacc gatgtcgtac cagagggcga
     1141 tcggacaaaa tggcaatcag agccatttac tatggtacag aaacaagatc ttctgatcgg
     1201 ccggggcgtc gaggacaatc accagggcct gacggccggc gtcttcgctc tgcaggcact
     1261 cagcgagtgc gccgttgtct cgaaccatga tgtgggaatg atcttcgtcg ccgacgagga
     1321 gtgtggatca cagtatggga ttcagtacct gctgcgagcc catcccgacc tcttcggtcc
     1381 acgagatgtc gtcctcgtgc ccgattcggg gaacagcgac ggcaccatga tcgaggttgc
     1441 cgagaaatcg attctctggt tgaagttcac caccaccgga aagcagtgcc acgccagtcg
     1501 cccctacaac ggaaaaaacg ctttcgctgc cgccgctgaa ctggtggtgt ctctcggccg
     1561 actccccgaa gtctggccac aga
//
LOCUS       DAORHD010001281         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_105931_length_1582_cov_3.088409, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001281 DAORHD010000000
VERSION     DAORHD010001281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105931_length_1582_cov_3.088409"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1582)
                     /locus_tag="PKO06_20865"
     CDS             complement(<1..>1582)
                     /locus_tag="PKO06_20865"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72172.1"
                     /translation="SIVREPGLHEHLTTLETATPSVRTQKLNAYFDRLVRKMQQNNLD
                     VRAILLYGPGEGAGYYSPQAKYSPATVNMINEFHLAFSLELLRRRFPGFVREEKRLGI
                     VRKASINAFISHGIGGTNQELFEALDTLSPFRSEGRQSLRYHNLILPESATDSRQIAP
                     YLMVILWDQNPGFQRYLSETTIEANRLQQAQTTGGAHIQTMALHQTPDGFKRISHDVP
                     HPEFASLLRKARYGGFLNTSGTWLHAGFPSTRMPGFFLAACSSLEFIDDRVRTETRRL
                     AGAFLLLILFLLTAGQALSIWLGSPIVRMTDGLRRVAADDLSVQVAERRQDELGRAGA
                     YLDEMIRSLKERQVMSRFVAPQVLDVVGQGNFHEAFQPQTREVALLVSDVRSFTTMSE
                     SHSPAEIFALINRHLQVMTRIIQKHGGAIDRFIGDAIQAVFYAGPGEHMVRRALKAAD
                     EMMTAHHTLIAERQRAGLFPYGIGVGVEIGEVVTGVLGDRDVRLDFTVMGEPFKHAAD
                     PEGASKKGKARLIICSDRV"
BASE COUNT          348 a          426 c          477 g          331 t
ORIGIN      
        1 acccgatctg agcagatgat gagtcgggcc tttccctttt tggaagcacc ctcaggatcg
       61 gcggcgtgct taaagggctc gcccatgacc gtgaaatcaa gacgcacgtc gcggtcaccc
      121 aggacaccgg tgacaacttc accgatctct acgccgacgc cgataccata gggaaagagg
      181 cccgcgcgct gccgctcggc aatgagggtg tgatgcgcgg tcatcatttc atcagcagct
      241 ttcagagccc gtcgcaccat gtgctctcct ggaccggcgt aaaaaacggc ttgaatggca
      301 tcgccgatga agcggtcaat ggcacccccg tgtttctgaa tgatgcgggt catgacctgc
      361 aggtggcgat tgatcagggc gaaaatttct gctggcgaat gggattcgga catggtggta
      421 aagctgcgga cgtcagaaac cagcagggca acttcgcgtg tctgcggttg aaaggcctcg
      481 tgaaagtttc cctgaccgac aacatcaagg acctgcggcg cgacaaaacg gctcatgacc
      541 tgtcgttcct tgagcgatcg aatcatctca tcgagatacg ctccagctct gcccagttcg
      601 tcctgtcgtc gttctgcgac ttgtactgac aagtcgtctg ccgcgactcg tctcagacca
      661 tcggtcatgc gcacgattgg ggagcccagc cagattgaca gcgcctgacc cgccgtcagc
      721 agaaagagga tcagcagcag aaacgctcct gccagtcgcc tggtttcggt gcgcacccgg
      781 tcgtcgatga actcgagtga tgagcaggcg gcgagaaaga atccgggcat gcgcgtactg
      841 ggaaagccgg catgtaacca ggttccggaa gtgttgagga acccgccgta tcgggctttg
      901 cgtaacagag acgcgaattc cgggtgcggg acatcgtgag agatgcgctt gaacccatca
      961 ggggtctggt gtagagccat ggtctgaatg tgagcgccgc cggtcgtctg tgcctgttgc
     1021 agccggttcg cttcgatcgt ggtttccgag agatagcgct gaaaacccgg gttctgatcc
     1081 cacagaataa ccatcaggta gggggcgatc tgccgagagt ccgttgctga ctcggggaga
     1141 atgaggttgt gataccgtaa cgactgccgc ccctcactgc gaaagggcga tagggtgtca
     1201 agagcctcaa acaactcctg gttggtcccg ccgattccgt gagaaatgaa ggcattgatg
     1261 gaagccttgc ggacgattcc tagacgtttt tcttctcgga caaatccagg aaatcgtctg
     1321 cgcaggagct cgagagaaaa ggcgagatga aactcgttga tcatgttgac ggtcgcaggg
     1381 ctatacttcg cttgtggcga gtaatacccg gcaccttcac ccggaccata cagaagtatg
     1441 gctcgaacgt ccaggttatt ctgttgcatc ttcctcacca gacgatcgaa ataggcattg
     1501 agcttctggg tgcgaacgga tggcgtcgcg gtttcgaggg tggtcagatg ctcatgcagc
     1561 ccaggttcgc gtacgatcga ac
//
LOCUS       DAORHD010001282         1581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106039_length_1581_cov_3.151376, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001282 DAORHD010000000
VERSION     DAORHD010001282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106039_length_1581_cov_3.151376"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKO06_20870"
     CDS             <1..218
                     /locus_tag="PKO06_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72173.1"
                     /translation="TDYLLNLSIWMSFLLVGLGVFVLWHPGRAYDETALAKEIRERLQ
                     QLEKDAGTGTPASTPAQTRSEPAKTES"
     gene            284..>1581
                     /locus_tag="PKO06_20875"
     CDS             284..>1581
                     /locus_tag="PKO06_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72174.1"
                     /translation="MSRNFVILTLIGFFCAGLFPVEGAVRKPNPSTNIVWQHSFGGTS
                     DDGAHCLRLTGDGGWVTAGFTQSTDGDIKEFAGKSDALVIKYDSAGKVAWSRVYGEGE
                     RDVAYSIALTSDGGYVFTGSTEHCTDEDAHYCGSNDLWVVRLTAGGEVLWQKCFGGNH
                     DEVGTGIQQTSDGGFIVVGTTCSSDGEEVGTTLGETDALVLKLDVNGELVWSKRLGGI
                     GDDVGYGVQQTTDSGYVVTGRIDVGPETLGALHGLGDVMIVRLDASGEVVWQQHYGGT
                     DEDIGYGVTQTTDGGFAVIGCTHSSDGDVRGNHGGNDVWALKLAASGTLEFQHCYGGV
                     DEEVGFAIHPLSDGDFIVAGQTHSVNGDLTTRNYRGADLWLLRLDGILNLRWQIPFGS
                     RKTDVGHDVQAMPDGTFLVAGSGSPRDEIDVAAGRTGSDV"
BASE COUNT          316 a          379 c          521 g          365 t
ORIGIN      
        1 tcacggatta tctgctgaat ctgtcgatct ggatgtcttt tctgctggtt ggtctcgggg
       61 tgttcgttct gtggcatcct gggcgggcct atgatgaaac cgcgcttgcc aaggaaatac
      121 gcgaacgctt gcagcagctg gaaaaggatg ccggcacggg cactcccgca tcgactccgg
      181 ctcagacgag atctgagccg gcgaaaaccg aatcctgaaa acctgtgcaa tcttgcggtt
      241 tgtgccgata aggctgacga attccttgtc gtgggagtct ctcatgagcc gcaatttcgt
      301 cattctgacc ctgatcggtt tcttctgtgc tggcctgttt cctgttgagg gtgcggtccg
      361 caagccgaat ccgtcgacaa atatcgtctg gcagcacagt ttcggaggta cctccgatga
      421 tggcgcccac tgtctgcggc tcacgggcga tggaggctgg gttacggccg gatttaccca
      481 gtcgacggac ggagacatca aggagttcgc cggcaagtca gatgctctgg taatcaagta
      541 tgactctgcc ggaaaagttg cctggagccg ggtttacggt gaaggcgagc gagatgtcgc
      601 ctacagtatt gcactgacgt ctgacggtgg ctatgtgttc accggcagca cggagcactg
      661 tacggatgaa gacgcccatt actgcggaag caacgacctc tgggtcgttc gcttgactgc
      721 cggtggtgag gtgttgtggc agaaatgttt cggtggaaac cacgatgagg tgggaaccgg
      781 cattcagcag acctcggacg gtgggttcat tgttgtcggt accacctgtt cgagtgacgg
      841 ggaagaggtg ggaacgaccc ttggggagac ggatgctttg gtcttgaaac tggatgtgaa
      901 tggtgagctt gtctggtcaa aacgcctggg cggtattggc gacgatgtcg gatacggtgt
      961 gcagcagacc acagatagtg gctatgtggt gaccggtcgc attgatgtgg gtccggaaac
     1021 acttggagct ctgcatggat tgggtgatgt gatgattgtc cgacttgatg cgagcggaga
     1081 agtggtctgg caacagcatt acggcggaac ggatgaggat ataggatacg gtgtcacgca
     1141 aaccaccgac ggagggtttg ccgtcatcgg ctgcacgcat tcttcagacg gcgatgtgcg
     1201 tggcaatcac ggagggaatg atgtctgggc gctcaaactg gctgcctcag gaacgctgga
     1261 gtttcaacac tgttatgggg gagtcgacga ggaagtgggg tttgcgatac atcctctcag
     1321 cgatggagac tttatcgtcg ccgggcagac gcattcggtg aatggtgacc tgacaacgcg
     1381 caactaccgc ggggccgatc tgtggctgtt gcgcctcgat ggcatcctga acctgcgctg
     1441 gcagattccc ttcggcagtc gcaagaccga tgtcggacac gatgttcagg ccatgccgga
     1501 tggaaccttt ctcgttgctg gcagtgggtc acctcgtgac gaaatcgatg tcgccgcggg
     1561 gcgtacgggt tctgacgtct g
//
LOCUS       DAORHD010001283         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106134_length_1580_cov_3.418361, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001283 DAORHD010000000
VERSION     DAORHD010001283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106134_length_1580_cov_3.418361"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PKO06_20880"
     CDS             <1..633
                     /locus_tag="PKO06_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72175.1"
                     /translation="GLLLTHFHNDRLEGAHAVLEHCRVDRVWENGSDIESPAFQDYRA
                     ICARLRVPRVPVKAGDTLDFGAEVFAAVLRPADGWLKAPKIEQHDGSIMLLLRYGKTS
                     FLFPGDVETDAQDKLAVLGDQLKAVGMLLPISGSAQAFSESFFRVVQPDFACISVGAD
                     NLAGFPETPVLNWFQKEKIEIYRTDSQGTITIRLGGRSTKDFQVRVDRSL"
     gene            709..>1580
                     /locus_tag="PKO06_20885"
     CDS             709..>1580
                     /locus_tag="PKO06_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72176.1"
                     /translation="MLESVLLKNPPPSPESHKTQWQKHINDHAGLKAIIETINAPKGK
                     ALIFSHDDPDGITSGLILKRTLTAKGWNAVLKLPEGFMLSPQQFEQALQEHPDTKAVF
                     ISDKGTLAPYNPYGEKLPVYIIDHHPSPQAPDSCKVYNPALASYIPASTSLLAHGIAT
                     LCGTREAFDDFLCLVGLKGDWTVEPIRFGVNGEFAKPFMVEYGMAFKKLFEAVNERPT
                     MFDAEQREGTCLLSRVAEFVHAVGGGGFSYFYHDRHESLKGVDHAACIASALEKMADK
                     SHDLVKLKSLEDFV"
BASE COUNT          351 a          448 c          419 g          362 t
ORIGIN      
        1 ggtttgctgc tgacacactt tcacaacgat cgccttgagg gcgctcacgc ggttctcgaa
       61 cactgtcgcg tcgaccgcgt ctgggaaaac ggaagcgata tcgagagccc ggccttccaa
      121 gattaccggg ccatctgtgc gcgcttgcgg gtgccacgcg tgccggtcaa agccggagac
      181 acactcgact tcggtgccga agtctttgct gcggttttgc gcccagctga tggttggctc
      241 aaagctccaa agatcgaaca gcacgacggg tcaatcatgt tgctgctgag atacggaaaa
      301 acctcgtttc tttttcccgg tgacgttgag accgatgccc aggacaaact ggccgttctc
      361 ggcgatcagt tgaaggcggt tggtatgctc ctccctatct ctggatcagc acaagcgttc
      421 tctgaatcat tttttcgcgt ggtccagcct gactttgcct gtatctcggt cggtgcggac
      481 aacctcgcgg ggtttcccga gacgcccgtc ttgaactggt ttcaaaaaga aaagattgag
      541 atctatcgca cggattcaca ggggacgatt accattcggc ttggcgggcg cagtacgaaa
      601 gactttcagg ttcgggttga ccgttcgctc tgatcttcct atactgatcc ggttacatac
      661 tgcaattggc cccgtccatt ctgcaaaccc acgagaaagg taatctacat gctcgaatca
      721 gtgctcctga agaaccctcc cccgtcacct gaatctcaca agacgcagtg gcagaaacac
      781 atcaacgatc atgccggtct caaggcgatt atcgaaacca tcaacgcgcc aaaagggaaa
      841 gcactgatct ttagtcatga cgaccccgac ggaatcacca gcggtctcat tctcaagcga
      901 acgctgacgg ccaaaggctg gaatgctgta ttaaagcttc cggaaggatt catgctctcg
      961 ccgcagcagt ttgaacaggc ccttcaggaa cacccggata cgaaggccgt gttcatctcc
     1021 gataagggga cgctcgctcc gtacaatccc tatggtgaaa aactgccggt ctacatcatt
     1081 gatcatcacc cgagtcctca ggctcccgat tcctgcaagg tgtacaaccc ggccctggca
     1141 agttacattc ctgcctcgac ctcactgctg gcacatggta tcgccactct gtgcggaacc
     1201 cgtgaagcct tcgatgattt cctgtgcctc gtcggtctca agggcgactg gaccgtcgaa
     1261 ccgattcgct tcggagtgaa cggcgagttc gccaagccat tcatggtcga atacggtatg
     1321 gcgttcaaga agctgttcga ggctgtgaac gagcgtccca ccatgttcga tgctgaacag
     1381 cgcgagggaa cctgtcttct gtcgcgggta gccgagtttg tccacgcggt tggcggcggt
     1441 ggattctcgt atttctacca tgatcgtcac gaatctctga aaggtgttga tcatgcagcc
     1501 tgcatcgcca gcgctctcga gaagatggcc gacaaatcgc atgatctggt gaagctgaag
     1561 tcgctcgaag acttcgtgaa
//
LOCUS       DAORHD010001284         1580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106163_length_1580_cov_2.843934, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001284 DAORHD010000000
VERSION     DAORHD010001284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106163_length_1580_cov_2.843934"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKO06_20890"
     CDS             <1..552
                     /locus_tag="PKO06_20890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72177.1"
                     /translation="LQTLTSKGMLRVDTGTAVRSSSTAADCQVLVGEGLVRSALMRDF
                     LKKELPKEILELNLRFDDSSIGINGRLDGPLFINPRFEARIEFAFVKTNSYRMNIRGL
                     KVMGFELKVFSSMLKKYVDDALKRVFIKGCTTRATDGADGSVAIDVTINPDGFVPGIG
                     QKAVLSGLELSGRMLRFSFTLTK"
     gene            complement(651..>1580)
                     /gene="cysK"
                     /locus_tag="PKO06_20895"
     CDS             complement(651..>1580)
                     /gene="cysK"
                     /locus_tag="PKO06_20895"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNV72178.1"
                     /translation="GRTPLVRINNLTRGLKATVLAKIEGRNPAYSVKCRIGAAMIWAA
                     ERDGHLTRGRTDVTIVEPTSGNTGIALAFTCAARGYPLILTMPDTMSIERRKMLAAFG
                     AQLVLTEGAKGMKGAIVRAEELVASDPKRYFMPQQFKNPANPEIHVKTTGPEIYEDTE
                     GKIDIFIAGVGTGGTITGVSRYLKQQRHLAVKTIAVEPALSPVLTQIRKGEEPKPAPH
                     KIQGIGAGFKPDILDMSLVDEIATVTNEEAIEYARRLHREEGITCGISCGAAMAVACR
                     VAADPANEGKTIVTILPDAGERYLTTALFEGFV"
BASE COUNT          310 a          436 c          471 g          363 t
ORIGIN      
        1 ctgcagaccc tgaccagcaa aggaatgctg cgtgtcgata ccggtactgc cgtacggtcg
       61 tcgtcgacgg cggctgactg tcaggtgctg gtcggcgaag ggttggtgcg cagtgctctg
      121 atgcgcgatt tcctgaaaaa ggaactgccg aaagagattc tggaactgaa tctgcggttc
      181 gatgattcat cgatcggcat caatgggcgt ctggatggcc cactcttcat caaccctcga
      241 tttgaggctc gcatcgagtt cgcgttcgtc aagacgaatt cgtatcgcat gaacattcgc
      301 gggctcaagg tgatgggatt cgaactcaag gtcttttcga gcatgctcaa aaagtatgtc
      361 gatgatgcac tgaaacgggt gttcatcaag ggttgtacca cgcgggcgac agatggcgct
      421 gacggatcgg tcgcgatcga tgtcacgatc aacccggatg gatttgtccc gggtatcggt
      481 cagaaggcag tgctgtcagg cctcgaattg agcggccgaa tgttgcggtt ctccttcacg
      541 ctgacaaaat agcctgacgc gcacggtcac gcagaagctg cgacaagaga aatcgggggc
      601 ggtgtttcaa aagacaccgc ccccgttgtt ctatcgcgtt ggtgaccgcc tcagacgaaa
      661 ccctcgaaga gagctgtggt caggtatcgt tcgccggcgt caggcagaat ggttacgatt
      721 gttttcccct cgtttgccgg gtctgcggct acccggcagg cgaccgccat ggcagcgccg
      781 cacgaaatgc cgcacgtgat gccttcttcg cgatgtagtc gccgggcata ttcgatggct
      841 tcttcattgg tgaccgtggc gatctcgtcc accaggctca tgtcgagaat atcaggtttg
      901 aaacccgcgc cgatgccctg aatcttgtgc ggagcgggtt tcggttcttc ccctttgcga
      961 atctgtgtca gcacaggtga gagagcaggt tcgaccgcaa tcgtcttgac ggccagatgg
     1021 cgctgctgct tgagataccg actgaccccg gtgatggttc cgccggttcc gacaccggca
     1081 ataaagatat cgatcttccc ctcggtatcc tcgtagatct cggggccagt ggtcttgaca
     1141 tgaatctcag ggttagcagg gttcttgaac tgttgtggca tgaagtagcg cttcggatca
     1201 gaggcgacga gttcctctgc ccgtacaatc gcgcccttca ttcccttcgc cccttcggtc
     1261 agtaccagtt gtgctccgaa tgcggccagc attttgcgcc gttcgatcga catcgtatca
     1321 ggcatggtga gaatgagcgg gtaacccctc gcagcgcagg taaacgcgag tgcgataccg
     1381 gtgttcccac tcgtgggctc gacgatcgtg acatccgtgc gaccgcgggt caaatgcccg
     1441 tcacgctcgg cggcccagat catcgccgcg ccgatacggc atttcaccga gtatgccggg
     1501 tttctcccct cgattttcgc gagcacggtg gccttgagac cacgggtaag attgttgatg
     1561 cgaaccagcg gtgttcgacc
//
LOCUS       DAORHD010001285         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106506_length_1577_cov_3.118922, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001285 DAORHD010000000
VERSION     DAORHD010001285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106506_length_1577_cov_3.118922"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKO06_20900"
     CDS             <1..736
                     /locus_tag="PKO06_20900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72179.1"
                     /translation="NPTNQVVAQILCRRLGIMLDLAQNGREAVEAVRTREYDLVLMDC
                     QMPECDGYEATREIRLLPGPKAQIPIIAMTAHTMETDRQRCLAAGMNAFVPKPVTLES
                     LSGVLKDWLSKQAPEAEVIPRSSGEIAKTESSEIWNPQPFLDTLQGDRETAAMALEVF
                     LAEFPKQLTDLRKALDDKDLTRVRRSAHRLKGSALELQIVRLRELALQIEKGPDDLVG
                     LENLYAQLEKSFQTFRDQAAQHSFFS"
     gene            783..>1577
                     /locus_tag="PKO06_20905"
     CDS             783..>1577
                     /locus_tag="PKO06_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72180.1"
                     /translation="MMRRSLAVQLFFLALFFALHGVTSVGAGSTTRDVWKKLTDCLQA
                     GKSEREQEAARLGVTLPSQMPSAYDVAPAKPQPSVEPAVEPAVEPTPRFQEWTKHEAD
                     AEVQRQAGASSDSSSSPQERTPTTREIILGYTESETPSPESAPPAAPAASPESAVSPE
                     PTPVPAVIQQGPQTFPAGEPKASDRMEVKPQPVAVSPEHEKAVATAKALGVPENRELG
                     EFLAYFPAVEDEPVQSQSAVSATEKAIQVPQVTLGTVVEVTESVPTA"
BASE COUNT          354 a          457 c          458 g          308 t
ORIGIN      
        1 caatccgacg aaccaggtgg tggcgcagat tctctgtcga cgacttggta tcatgcttga
       61 cctggcacag aacggccgcg aagcggtcga ggctgtgaga acgcgggaat acgacctcgt
      121 gttgatggat tgtcagatgc ccgaatgtga cggatatgaa gcgacccgcg agatacgcct
      181 gcttccgggc ccgaaagcac agatcccgat catcgcgatg acggcacaca ccatggaaac
      241 agaccggcaa cgctgtcttg ccgccggcat gaatgccttc gtgcccaaac cggtaacgct
      301 cgagagtctc tcgggagtcc tgaaagattg gctttccaag caggcgcctg aggcagaggt
      361 gattcctcgg tcgtcaggag agatcgccaa gacggagtct tccgagatct ggaacccgca
      421 gccgtttctc gacacccttc agggcgatcg cgaaactgcg gcaatggctc tcgaggtctt
      481 tctggcggag tttcccaaac aactgactga tctccggaag gcgctggatg acaaggatct
      541 cacccgggtg cgccgcagtg cccatcgttt gaaggggagc gctctcgaac tgcagattgt
      601 ccgtttgcgc gagctggccc tgcagattga gaagggccca gacgatctgg tcgggctcga
      661 gaatctctac gcacaactgg aaaagtcctt tcagaccttt cgtgaccagg cggcgcaaca
      721 ttcgtttttt tcgtgacgaa aaaattgcat tcgtctgccg aaagaatgat aatagaggta
      781 ctatgatgcg cagatcactt gcggtacaat tgttctttct ggctcttttc ttcgctcttc
      841 acggcgtgac aagtgtcgga gcaggttcca cgacacgcga tgtctggaaa aagctgactg
      901 attgtctgca ggccgggaag tcggaacgag aacaggaagc ggcccggttg ggcgtcacgc
      961 ttcccagtca gatgccatca gcctatgatg tcgctcctgc caaaccgcaa ccatcggtcg
     1021 aaccggcagt cgagccggca gtcgagccga ccccccgttt ccaggaatgg accaaacacg
     1081 aggctgacgc agaagtgcag cggcaagccg gcgcatcgag cgattcatca tcttcacctc
     1141 aagagcgcac acccacaact cgtgagatca tattgggata cacagaatca gagactccct
     1201 ctccggaatc cgccccgccg gcagctccag cggcttctcc tgaatcagca gtttcaccag
     1261 agccgacgcc ggttccggcg gtgatccagc agggaccgca gacgttcccg gctggtgagc
     1321 cgaaagcctc tgatcgcatg gaagtgaagc ctcaaccggt tgcggtttct cccgaacacg
     1381 aaaaagctgt cgccactgcg aaggctctgg gagtgccgga aaatcgggag ttgggcgagt
     1441 tcctcgcata cttccccgcg gttgaagatg aaccggttca gagccagagt gccgtttccg
     1501 caactgagaa ggcgatccag gttccccagg tcaccctcgg aacggtcgtg gaagtcactg
     1561 agtcggttcc gaccgcc
//
LOCUS       DAORHD010001286         1577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106531_length_1577_cov_2.595926, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001286 DAORHD010000000
VERSION     DAORHD010001286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106531_length_1577_cov_2.595926"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKO06_20910"
     CDS             complement(<1..276)
                     /locus_tag="PKO06_20910"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV72181.1"
                     /translation="MRSSSRWFVSSLIGLALSATAWAGAPTTPESFPPDFTWGTVLSA
                     HSVEGGHQNNNWAAWEATPGHVPGNAVSGAAANHWELFREDIRWMKEL"
     gene            428..>1577
                     /locus_tag="PKO06_20915"
     CDS             428..>1577
                     /locus_tag="PKO06_20915"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV72182.1"
                     /translation="MLLKRIWFGLILAIIIFINPWTRDLERWSVDERFQWRGVRSPLA
                     DLIIVKIDAPTLQWANTPFWGFKPLYEEFLTAMCSVQARAVFLDLIFDVSPDPIIQQL
                     VGKFCEANQIAFPPHLLNRLGFDLPFRQALVKARQAGVKVILGYNKEQQGMAATHDLI
                     HLTTPEDQKGFLNMLTDSDHVIRRALVTAVETQTERWYPSVARVMAWQIASECVLPPS
                     PEILINYAGPAGTYQTESFMNVLNTFRQKPNSLERFRGKTVLLGLGDITDLKAVPWKG
                     FMNGVEIHANIVENLVRANWLRRAPPWVSPVFMLLVFPLQILLFQRRGWIGGLVTLVI
                     SVVWVASAQVLFAQQVACIRQRETGQRVGRRKGQYCRLLAGGDDQAQPL"
BASE COUNT          310 a          439 c          464 g          364 t
ORIGIN      
        1 gagttctttc atccagcgaa tgtcttcgcg gaagagttcc cagtggttgg ccgcggctcc
       61 gctgacggcg ttgccgggaa catggcccgg agtggcttcc caggccgccc agttgttgtt
      121 ctggtgcccg ccttcgaccg aatgcgctga cagcacggtt ccccacgtga agtcgggggg
      181 gaaggattcc ggtgtcgttg gtgccccggc ccaagccgtc gctgaaagag cgagaccgat
      241 aagtgatgat acgaaccaac gggacgaaga acgcatgtgc gacctcccgg tcagaatctt
      301 gtactaattc tattcatctt aacaaacgcc ggcgacaacc gtcaaaggtc gctcgagaat
      361 tgagaatgtg ttactctggc ccaggtaccg tattgctctc attctatctg tcagggaacg
      421 gcgacccatg cttctgaagc gaatctggtt tggcctcata ctggcaatca ttatcttcat
      481 caacccctgg acgcgagacc tcgagcgttg gagcgtcgat gagcggtttc aatggcgtgg
      541 ggtccggtct cccctcgctg atctgatcat tgtgaagatt gatgctccga cgttgcagtg
      601 ggccaacacg cctttctggg gcttcaaacc cttgtatgaa gagtttttga cggccatgtg
      661 ctcggtacag gcgcgagcgg tctttctcga cctgatcttc gatgtcagcc ctgatccgat
      721 cattcagcag ttggtcggca aattctgcga agccaatcag attgcctttc ccccgcactt
      781 gctgaaccgt ctcggcttcg atctgccctt ccgacaagcc ttggtcaagg cgcgccaggc
      841 gggggtaaaa gtcattctcg ggtacaacaa agagcagcag ggaatggcgg cgacgcatga
      901 cctgattcac ctgacgaccc ccgaagacca gaaggggttt ctgaatatgc tcaccgattc
      961 agatcacgtg attcgtcgtg ctctcgtgac cgcggtcgag acccagaccg aacgctggta
     1021 tccatcggtg gcgcgcgtga tggcctggca gattgcgtcg gaatgcgttt tgccaccatc
     1081 tcctgagatc ctgatcaact atgctggccc ggccggaacg tatcagacgg aatcattcat
     1141 gaacgttctc aacacctttc ggcagaaacc gaactctctc gagcgatttc ggggaaagac
     1201 ggtgcttctg ggactcggcg acattaccga tctgaaggca gttccctgga aaggattcat
     1261 gaacggggtc gagattcacg ccaacatcgt cgaaaacctg gtgcgagcca actggctgcg
     1321 acgagcgccg ccctgggtat cccctgtctt catgctgctg gtgttcccgt tgcagattct
     1381 gctgtttcag cgtcgtggct ggattggtgg tctggtcacg cttgttatct ccgtggtctg
     1441 ggtggcaagc gcccaggttc tgtttgcgca gcaggtcgcg tgtatccgcc aacgagagac
     1501 cggtcaacgc gtgggccggc gcaagggcca gtactgtcgg cttttggcgg gaggtgatga
     1561 tcaagcgcag ccgttgg
//
LOCUS       DAORHD010001287         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106593_length_1576_cov_4.247863, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001287 DAORHD010000000
VERSION     DAORHD010001287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106593_length_1576_cov_4.247863"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="PKO06_20920"
     CDS             <1..687
                     /locus_tag="PKO06_20920"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNV72183.1"
                     /translation="FCLGGDTSFGADASFSCTALGRVPAGRKPTLRLGARAGDLLYTT
                     GPLGSGNLLAISAQVNPDAWSKLEKVYRPVARLREARALSSLVHCSIDTSDALLQALA
                     IIASLNQVGIEFEQRDELYDPDLRALSAAIKLPLWLVNVFGLGEYELVWSVDASREEA
                     FLQKAQAERIPVNRIGRVSAAPGIRLQESNRVFTLDVPYLLNLFGTCPSIPDYLKTLI
                     TYDATLRSQK"
     gene            684..1493
                     /locus_tag="PKO06_20925"
     CDS             684..1493
                     /locus_tag="PKO06_20925"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV72184.1"
                     /translation="MSFAGRRILIVGGSSGIGLALAKALDRQGSHLILYSRHVDRLRA
                     DTHGTFADRHTLVTGDLRHPEDLNRLDAAIANAGGLLDGIIFSAGVSRPDDIDALDLE
                     RALDTIRLNLEAPFRVFYRYLPQLLKRPGSFLAGFTSMAGDRGMPKGHAYSAAKAGFD
                     RLLESLRIDLLDRGITVYTIVPGYVDTPMSAQNRFPMPGIWPPERAAEHILQAMSRGK
                     QVIRFPWYHSLGMRLLCALPDRLYWWLMHRQRSQIKIDPHPDEPLRWPSEP"
BASE COUNT          281 a          528 c          439 g          328 t
ORIGIN      
        1 ttctgcctcg gcggtgacac gagtttcggg gcagacgcca gcttttcctg cactgctctg
       61 gggcgggttc ccgccggacg caagcccacc ctgcgactcg gcgctcgggc gggcgacctg
      121 ctgtatacca ccggccccct tggcagcggc aatcttctgg ccatctcggc gcaggtcaat
      181 cctgacgcgt ggagcaaact cgaaaaagtc taccggccgg tcgctcgtct gcgagaagct
      241 cgcgctctga gttccctggt acactgcagc atcgacacga gcgacgctct actgcaagca
      301 ctcgccatca tcgcttcgct gaatcaggtt ggcatcgagt ttgagcaacg cgacgaattg
      361 tacgatcccg atctgcgcgc tctgtcagca gcaatcaagc tgccgctctg gctggtcaac
      421 gtcttcggac tcggcgagta tgaactggtc tggtcggtcg acgccagccg cgaagaagct
      481 tttctgcaga aagcccaggc cgagcgcatt cctgtcaacc gtatcggtcg ggtctccgcg
      541 gctccaggaa tccgcctgca ggagagcaac cgggtgttca cgctcgatgt tccgtatctg
      601 ctgaacctct tcggtacgtg cccatcgata cccgactatc tgaagaccct catcacgtat
      661 gatgccaccc tgcggagtca gaagtgagtt ttgccggcag gcgcattctc atcgtcggcg
      721 gttcgagcgg catcggcctg gctctggcca aagccctcga tcggcagggc agtcatctga
      781 ttctgtattc acgacacgtc gaccgcctgc gagccgacac acacggaacg ttcgccgacc
      841 gacacactct ggtcaccggc gacctgcgac accccgagga tctgaaccgt ctcgatgcgg
      901 ccatcgccaa tgccggcggc cttctcgatg gcatcatctt ttcggcgggg gtctcgcgcc
      961 ccgatgatat cgatgcgctc gatctcgaac gtgcactcga tacgattcgc ctcaatctcg
     1021 aagccccatt tcgcgtcttc taccgatatc tgccacagct gctcaaacgc cctggcagct
     1081 ttctcgccgg cttcaccagc atggcgggcg accgcggtat gccgaaaggg catgcctact
     1141 ccgcggcgaa agccggtttc gatcgtctgc tcgagtcact gcgcatcgat ctgctcgatc
     1201 gcgggatcac cgtctatacg atcgttcccg gctacgtcga tacgccgatg tcagcccaga
     1261 accgttttcc gatgcccggc atctggcccc cggaacgcgc cgccgaacac attctgcagg
     1321 ccatgtcacg cgggaaacag gttatccggt ttccctggta tcactcgctt ggcatgcgct
     1381 tgttgtgcgc tttgcccgat cgtctctact ggtggctgat gcaccggcag agatcccaga
     1441 tcaagatcga tccgcatccg gacgaacctc tacgatggcc ctccgaaccc tgatcattgg
     1501 cgtcaacggc ttcgtgggca gtgccctcgc ccgtcacctg accgcggcag gcatttccgt
     1561 gacgggaacc atgctg
//
LOCUS       DAORHD010001288         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106630_length_1576_cov_2.996055, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001288 DAORHD010000000
VERSION     DAORHD010001288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106630_length_1576_cov_2.996055"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKO06_20930"
     CDS             complement(<1..403)
                     /locus_tag="PKO06_20930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72185.1"
                     /translation="MDLGQLDKMTRDFADARATLEGRVTELNDAIDTIKRKYLPLIKH
                     AVTTALDRKSKLEGEIEKNPQLFVKPRMLILHGIKIGLQKTRDGLAWEDDDQVVKLIK
                     KHFSDQVETLVKVKETPVKQALAQLSVADLRK"
     gene            complement(454..921)
                     /locus_tag="PKO06_20935"
     CDS             complement(454..921)
                     /locus_tag="PKO06_20935"
                     /inference="COORDINATES: protein motif:HMM:NF018011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HNV72186.1"
                     /translation="MASREQIRIIHSLKSALKLTEDEYRSLLSGYEVESSKDLSFETA
                     ADVIRSMMKNAQSAGVWVQKPGRSDRSERYEHLDGRARNMASPAQLRMIEGLWNDVSY
                     VRDPDKQRKALRKFLERFDVSALEFVQASLVPKILRALEYMKHKPSKAPKKAA"
     gene            complement(931..>1576)
                     /locus_tag="PKO06_20940"
     CDS             complement(931..>1576)
                     /locus_tag="PKO06_20940"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72187.1"
                     /translation="IFEVAKLCQIDGDIGVAIGEPGIGKTVSVEAFAAQNSGVILLEA
                     DPGMPVKACFAKMQRQFGGDGYGTAYTLFEDVLVRLNNSGRLIVIDEAEHLPHGALEL
                     LRRLHDKAGVGILLVGSPVLLGKLRGCRGQYTQLYSRVGVLASLKPINAADAEAIAEA
                     ALPGVGHARTLHEKTLGNARKLNKLAKRSSRISELNALPIDATVIEAAMTTLLV"
BASE COUNT          271 a          475 c          467 g          363 t
ORIGIN      
        1 gcttgcgcag atcggccacc gagagctgag ccagcgcctg tttcaccggg gtttccttca
       61 ccttgaccag cgtctcgacc tgatcggaaa aatgcttctt gatcaatttc accacctggt
      121 cgtcgtcttc ccaggcgagg ccgtcgcggg tcttctgcag gccgatcttg atgccgtgca
      181 gaatcagcat gcgcggcttc acgaagagct gcgggttctt ctcgatctcg ccttcgagct
      241 tcgacttgcg gtcgagagcg gtggtcaccg catgcttgat cagcggcagg tatttgcgct
      301 tgatcgtgtc gatcgcgtcg ttcagttcgg tcacgcggcc ttcgagcgtg gcgcgggcat
      361 cggcgaaatc gcgggtcatc ttgtcgagtt gtccgaggtc catggtcatt ttctccagtg
      421 ttgttgaggt tttaacgccg cgataaaccg gcgttatgcg gccttcttcg gggctttgct
      481 cggtttgtgc ttcatgtatt caagtgcccg cagaatcttg ggcaccaggc tggcctgcac
      541 gaattcgagc gccgagacgt cgaaccgttc gagaaacttg cgcagggctt tgcgctgttt
      601 gtccgggtcg cgcacatacg agacgtcgtt ccagagacct tcgatcatgc gcagttgcgc
      661 gggtgacgcc atgttgcggg cgcgtccgtc gagatgctcg tagcgttccg accggtcaga
      721 acgtccgggc ttctgaaccc acacaccagc gctctgagcg ttcttcatca tgcttctgat
      781 gacgtccgcc gccgtctcga acgacagatc tttcgagctt tcgacctcat accctgacag
      841 caggcttcgg tattcgtcct ccgtcagctt cagagcgctc ttgagcgagt gaatgatgcg
      901 gatctgttct cggcttgcca tggtgcctcc tcaaaccagc agggtcgtca tcgccgcttc
      961 gatcacggtt gcgtcgatcg gcaaggcgtt caactcggaa atccggctcg atcgcttcgc
     1021 gagcttgttg agctttcggg cattcccgag agttttctcg tgcagcgtgc gggcatgacc
     1081 gaccccggga agcgctgctt cggcaatcgc ttcggcatca gcggcgttga tcggcttcag
     1141 actggccagc acgccgacac ggctgtacag ctgcgtgtat tgtccgcgac agccgcgcaa
     1201 cttcccgagc agcacagggg agccgacgag aagaatgccg acaccggctt tgtcatgtag
     1261 gcgacgcaga agctccagtg cgccgtgcgg caggtgttcg gcctcgtcga tcacgatcaa
     1321 acgcccggag ttgttcaggc gcaccaggac atcttcaaag agggtatacg cggttccgta
     1381 tccatcgccg ccgaactgcc tctgcatctt ggcaaagcat gccttgaccg gcattcccgg
     1441 gtcagcctcc agcagaatca cgccggagtt ctgtgcggca aaagcctcga cagatacggt
     1501 tttgccgatg ccaggttcgc cgatggccac gccgatgtcg ccgtcgatct ggcagagctt
     1561 ggcaacctcg aagatc
//
LOCUS       DAORHD010001289         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106856_length_1574_cov_3.013167, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001289 DAORHD010000000
VERSION     DAORHD010001289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106856_length_1574_cov_3.013167"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1574)
                     /locus_tag="PKO06_20945"
     CDS             complement(<1..>1574)
                     /locus_tag="PKO06_20945"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HNV72188.1"
                     /translation="LQSMMQEFYRLTHMGIGIIDIEGNVLVGVGWQDVCTKFFRAHPE
                     TCKCCLESDCTLSRGIPAGTYKAYHCKNHLWDLATPIEVDGQHLGNVFLGQFFYDDEV
                     PDEDLFRRMAHQYGFDETAFLAAVASVPRWSRETVDATMTFYTKLAGMISTLSYHKVR
                     LSQVLVEKSATLSELKESRKRLYAILSSIHSGILVVQEEGTLEFVNQAFCELYAISDD
                     PDSLRGMSSADFIRKIEQGFADPVRAVTRIREILQRNELIIGEEVALRGGHIHLRDCV
                     PIVINGKRCGRIWYARDITALKQAEAEAKRSEAALQRQLGLFKSLMENLPVGVFMVEA
                     PSGKPLLANEAALRLLGRGVLPDASRTNLAEVYKASKLSTHEPYPLDELPILRGMNGT
                     SSYVDDMLVERPDGTKTILEVFGSPVRNEQGQVWASLVSFNDISRRRQDEETIRRNED
                     RLRGLVNILQYRMSTIQEFLDMALNEVIRLTDSKIGYIYFYHEDRRQFVLNTWSKEVM
                     QQCSVVNPQTTYDLDQ"
BASE COUNT          344 a          443 c          436 g          351 t
ORIGIN      
        1 tctgatcgag gtcgtaggtc gtttgcggat tcaccaccga acactgctgc atcacctctt
       61 tcgaccaggt gttgagaacg aactgccggc gatcttcgtg atagaagtag atatatccga
      121 ttttgctatc ggtaagtcgt atgacctcgt tcagagccat atccaggaat tcctgaatag
      181 tactcatgcg atactgcaga atgttgacca atccccgcag gcggtcttcg tttcggcgaa
      241 tcgtctcttc gtcctgacga cgtcggctga tgtcattgaa actgaccaga ctcgcccaga
      301 cctgcccctg ttcgttgcgt accggagacc cgaagacctc cagaatggtc ttcgtgccgt
      361 cggggcgctc gaccagcatg tcatcgacat aggaacttgt cccattcata ccacgcagaa
      421 tgggcagttc atcgagcgga tacggctcat gagtgctcag tttcgaggcc ttgtagactt
      481 ccgcgagatt ggtacgcgaa gcatccggaa gaaccccccg acccagcagc cgcagggcgg
      541 cttcattcgc caggaggggc ttgccgctcg gcgcttcgac catgaagact ccgacgggaa
      601 gattttccat cagggatttg aacagaccta actggcgctg cagagcggcc tcgctccgtt
      661 ttgcctcagc ttcggcctgc ttgagcgcgg tgatatcgcg agcgtaccag atacgcccgc
      721 atcttttgcc gttgatgaca atgggaacac aatcgcgaag gtgaatgtgt cccccgcgaa
      781 gagcaacttc ttcgccgatg atgagctcgt tccgctgcag aatttctctg atacgggtga
      841 ccgcccggac aggatcggcg aagccctgct cgattttccg gataaagtcg gccgaggaca
      901 ttccccgcaa gctgtccggg tcatcggaga tcgcgtaaag ctcgcaaaac gcctgattga
      961 cgaactcgag cgtgccttcc tcctgaacca ccaggatccc ggaatggatg gaagaaagga
     1021 tcgcatacaa tcgctttcta ctctctttaa gttcgctcag agtagcgctt ttctcgacaa
     1081 gaacctggga caaacgcacc ttgtggtaac tcagagtcga gatcatgccg gcgagtttcg
     1141 tatagaacgt catggtggca tcgacggttt cgcggctcca gcgagggacg ctggcgacgg
     1201 ccgcgagaaa cgccgtttca tcgaacccgt actggtgcgc catgcgtctg aacaggtctt
     1261 catcaggaac ttcatcgtcg tagaagaact gcccgagaaa gacattccca agatgctgtc
     1321 cgtcgacttc gatgggggtc gcgagatccc agaggtggtt cttgcagtgg taggccttgt
     1381 aggtaccggc cggaataccg cgactgaggg tgcaatcgct ctcgaggcag catttgcacg
     1441 tctcagggtg cgcgcggaaa aatttcgtac agacgtcctg ccagccgaca ccgacgagaa
     1501 cgtttccttc gatatcgatg atgccgatac ccatgtgggt cagccggtag aactcctgca
     1561 tcatgctttg caag
//
LOCUS       DAORHD010001290         1574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106857_length_1574_cov_3.003950, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001290 DAORHD010000000
VERSION     DAORHD010001290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106857_length_1574_cov_3.003950"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PKO06_20950"
     CDS             complement(<1..624)
                     /locus_tag="PKO06_20950"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV72189.1"
                     /translation="MYKTIDKYILEELLQPFLFGLAFFVTIWLIDLMMELINLIFSKG
                     VPASVTAFLFLYNLPPTLVISFPMAILLANLIAFGRMSSDSEIIALKAGGYSFRRIVT
                     PAIAFGIFITGLTFLFNEKVVPVSNDKFAKLFRREVTLKRPLPKISANRFFEAGPGRK
                     FFVQRYDEPERMMLGVVMYEGQPKNFPRIIEAKRARLEEGRCLFWDGR"
     gene            924..>1574
                     /locus_tag="PKO06_20955"
     CDS             924..>1574
                     /locus_tag="PKO06_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNV72190.1"
                     /translation="MMDGNTAVAYTAHATNEVICIYPITPSSNMGEAADEKSAAGEKN
                     IWGSVPVVVEMQSEGGAAGAVHGSLTSGALTTTFTASQGLLLMIPNMYKIAGELTPTV
                     FHIAARSLACQGLSIFGDHSDVMACRQTGWAMLASNSVQEAMDFALISQTASLESRVP
                     FLHYFDGFRTSHQVDKVELINFDVMRDMLPDALIQAHRDRGLNPDRPMMRGTAQNPD"
BASE COUNT          365 a          428 c          457 g          324 t
ORIGIN      
        1 gcgaccgtcc cagaaaagac atcgaccttc ctcgagtcgc gcccgcttgg cttcgatgat
       61 gcgaggaaaa ttcttcggct ggccttcgta catgacgacg ccgagcatca tgcgctctgg
      121 ttcatcatag cgctggacga aaaactttcg tccgggaccg gcttcgaaaa accgattggc
      181 cgaaatcttg ggcagaggcc gtttcagcgt gacttcacgg cgaaagagct tggcgaactt
      241 gtcgtttgac acggggacga ccttctcatt gaaaagaaac gtcagtcccg tgatgaagat
      301 accgaaggcg attgccgggg tgacgatgcg tcgaaaactg tagccgcctg ctttcagagc
      361 gatgatttcg gaatcagaag acatgcgccc gaaggcgatg agattggcca gcaggatcgc
      421 catgggaaag ctgatgacga gggtcggcgg caggttgtac aaaaacagga acgcggtcac
      481 tgaggccggg acccctttgg aaaagatcag attgatcagt tccatcatga ggtcgatcag
      541 ccagattgtg acgaagaacg ccagtccgaa gaggaagggc tgcagcagtt cttccaggat
      601 atacttgtcg atggtcttgt acaatgaggg aatttcccag cacggtctga agtggtgaac
      661 actgtaccgt aatccgttcg gtcgtgcaag atgacagaaa accccgccgg aaacactcag
      721 gaacggaaag tttgagattt ttttcccaaa tcaggaaacc ttgggctgat cagggtttgt
      781 ggggtttgag ggtcgaggaa aaccttgaca tcgcgtacca atgggactat catggcccac
      841 cgtaaagccg gccgagaatt gggcggccga aaaatctcac agcctggagg aataccgtgg
      901 cttcagcgaa tcagaagttt gccatgatgg atggcaacac cgcagtggcg tatacggccc
      961 atgcgaccaa cgaagtgatt tgtatttatc cgatcactcc cagctcgaac atgggtgagg
     1021 ccgccgacga gaagagcgcc gccggcgaaa agaacatttg gggtagcgtt ccggtcgtgg
     1081 tcgaaatgca gtccgaaggc ggcgccgccg gtgccgttca cgggtcactc accagcggag
     1141 ccttgactac cacgttcacc gcctctcagg gtctgctgct catgattccg aacatgtaca
     1201 aaatcgcggg cgaactgacc ccgaccgtgt tccatattgc cgcgcgttca ctggcctgcc
     1261 agggtctttc gatcttcggc gaccattccg atgtcatggc ctgccgccag accggttggg
     1321 cgatgctggc ttccaacagc gttcaggaag ccatggactt cgcgctcatt tcacagaccg
     1381 cgtcgctcga gagccgcgtg cccttcctgc actattttga cgggttccgc acctcgcacc
     1441 aggtcgacaa ggttgagctg atcaacttcg atgtcatgcg cgacatgctt cccgatgcgc
     1501 tgattcaggc ccatcgcgat cgcggtctca accccgatcg gcccatgatg cgcggtacgg
     1561 cgcagaatcc cgat
//
LOCUS       DAORHD010001291         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106928_length_1573_cov_5.258893, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001291 DAORHD010000000
VERSION     DAORHD010001291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106928_length_1573_cov_5.258893"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PKO06_20960"
     CDS             <1..618
                     /locus_tag="PKO06_20960"
                     /inference="COORDINATES: protein motif:HMM:NF025623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HNV72191.1"
                     /translation="EENRENPKAKAFLASIESATPEKAETVLAPQLDPGTVREYLASK
                     DAFPALQPYLDKAKNEAILRFREHELPKLQEEYFAAKYSEKHPPQTEAEKQLAEITRK
                     FAEMEREKKLADIKAKALQAAAGKKLPAEIIDVLHLDDEEPTLARIEKLAELMSNQTN
                     AMVEERMKGVKPEPRTAGTVGKVGADAPIELQIQSALESFNQKGA"
     gene            622..>1573
                     /locus_tag="PKO06_20965"
     CDS             622..>1573
                     /locus_tag="PKO06_20965"
                     /inference="COORDINATES: protein motif:HMM:NF036552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HNV72192.1"
                     /translation="MPILAPGIITSYDVPENARDISPLFYGLTLPETVLFNRLGVGTR
                     AINKTLNWYDDELMPTSTTLGAAYTANQGTMTLADANFFPVGTIIRCEGSVYRVTDVN
                     LNTKKITIATLSGDANHESGKTVEIIGNGQLEGKAFGNTASVKRVERTNYCQIFEQTA
                     EVSGTEGATQLVNGTTGKMSEEIAKKMEYLYRLMGRAIWNGIALFASDNTIPRLMSGV
                     KTYIEANGYAPSAASFNQANFDAFLDEISKRGGSLIDIWCSPTVARKFSALKDDNVVA
                     ERTDGTVGRWTRSYLSGNGATLTVNSDPACLDTDAIYVFPT"
BASE COUNT          407 a          453 c          420 g          293 t
ORIGIN      
        1 gaagagaacc gcgagaatcc gaaagcaaag gctttcctcg cctccatcga gtcggcgaca
       61 ccggaaaagg cagagaccgt cctcgcacca cagctcgatc ctgggacagt gcgcgagtac
      121 cttgccagca aagacgcgtt cccggcattg cagccgtatc tcgacaaagc gaagaacgaa
      181 gcgatcctgc gttttcgcga gcatgagctc ccgaaacttc aggaggagta ttttgccgcg
      241 aagtattcag agaagcatcc acctcagact gaggcggaga agcagctcgc agagatcacg
      301 cgcaagtttg cagagatgga acgcgagaaa aaactcgcag acatcaaggc gaaggcgctg
      361 caggccgctg caggcaagaa acttcctgcc gaaatcatcg acgttttgca cctggacgac
      421 gaagagccaa cactcgctcg cattgaaaag ctcgcagagc tcatgagcaa ccagacgaac
      481 gccatggtcg aggagcgcat gaagggcgta aagcccgaac cgagaaccgc cgggactgtc
      541 ggcaaagtcg gggcagatgc tccgatcgag ctgcagatcc agtccgctct cgaatcattc
      601 aaccaaaaag gagcataaca aatgcctatt cttgcacccg gtatcatcac gtcctatgac
      661 gtgcctgaga acgcgagaga catttcgccc ttgttttatg ggctgacgct tcctgagacg
      721 gtccttttca accgcctcgg agtcggcact cgcgcaatca acaagaccct caactggtat
      781 gacgatgagc tcatgccgac cagcaccaca ctcggtgctg cctacactgc taatcaaggg
      841 acaatgactc tcgcagacgc gaacttcttc cctgttggca cgatcatccg ctgcgaaggc
      901 tctgtgtatc gcgtgactga tgtcaacctg aacacgaaaa agatcactat cgccactctc
      961 tctggtgatg caaaccacga atccggaaag actgtcgaga tcatcggaaa tggccagctc
     1021 gaaggcaagg cgttcgggaa tactgccagt gtcaagcgtg tcgaacgcac gaactactgc
     1081 cagattttcg agcagacagc cgaagtctca ggcactgaag gcgcgacgca gcttgtaaac
     1141 ggaacgacag gcaagatgtc tgaagagatc gccaaaaaga tggagtacct gtaccgtctc
     1201 atgggccggg ccatctggaa cggcattgct ctctttgcca gcgacaacac catcccccgc
     1261 ttgatgtcag gagtgaaaac atacatcgaa gcgaacgggt acgccccgtc tgctgcatcg
     1321 ttcaaccagg caaactttga cgcgttcctt gatgagatca gcaagcgtgg tggctcgctc
     1381 atcgacatct ggtgcagccc gactgtggcc cgcaagttct cagccctgaa ggacgataac
     1441 gttgtcgcag agcgcactga cggaactgtc ggtcgctgga ctcggtcgta cctgtctggc
     1501 aacggcgcta cgttgactgt caactctgac ccggcatgct tggacactga tgcaatctac
     1561 gttttcccga cat
//
LOCUS       DAORHD010001292         1573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_106930_length_1573_cov_4.654809, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001292 DAORHD010000000
VERSION     DAORHD010001292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106930_length_1573_cov_4.654809"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1573
                     /locus_tag="PKO06_20970"
     CDS             <1..>1573
                     /locus_tag="PKO06_20970"
                     /inference="COORDINATES: protein motif:HMM:NF019158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HNV72193.1"
                     /translation="LQSGRGAISLQSGSEAISNATAPDQEVLTDIGGLVVRFHDVLRT
                     CIGKWLPLPYHRKGAAAGSPCRSLDWVRVMVQRPPLQTDDRLYRVVLAVDTRLQPAGR
                     ADVSGFANEDVGFPFELNPGSIVFWQNPGLLSWVKHLFQMTPVDGSDKAPPFAAAMAS
                     YMALMDGFQQAEILPEITFLHPEGEVVDVSLVLDLGNSRACGILAEQVPGTPVSLDEC
                     CKLEIRDLRDPSQVYTEPFDTSFKFQPPLFQEPDNAIPQAGGGFSWPSLVRLGQEAAK
                     LEPCDVGDTGMSSPKRYLWDENQRHFPWYLNLADAGLGKKISAGFLKFLDENGVFRGD
                     KAQPPFEPCYPPSSLMTFLMMEILNHAFAQINSYSFRKSRGHRLACRVLKNVVITTPC
                     GMSKPEREKYRQRVQSAIDTVFHVMQLPAERKPKLFLDFDEATAIQLTYLFGEVKHRF
                     LGDARDAIATLGAERRTSSGAREPVLRIASIDIGGGTSDLMIAEYAQASSEASGVRQR
                     MLFSEGFSVAGDEIAK"
BASE COUNT          289 a          474 c          489 g          321 t
ORIGIN      
        1 ctgcagtcgg ggcggggagc catcagcctg cagtcaggca gcgaggcgat cagtaacgcc
       61 acggctcccg atcaggaagt cctcaccgat atcggcgggc tcgtggtccg gtttcacgac
      121 gtgctgcgca cctgcattgg caagtggctg ccgctgccct atcaccgcaa gggtgccgcc
      181 gcgggttcgc cctgtcgcag tctcgattgg gtccgggtca tggtgcaacg cccaccgctg
      241 cagaccgacg atcgcctgta ccgtgtggtt ctcgccgtcg acacccgtct gcaaccggca
      301 ggccgtgctg acgtgtccgg gtttgccaac gaggatgtgg gcttcccatt tgagttgaac
      361 cctggttcga ttgtcttctg gcagaacccc gggctgttga gctgggtgaa acatctgttt
      421 cagatgacgc cggtggacgg cagcgacaag gctccgccat ttgcggcggc catggcttcc
      481 tatatggccc tgatggatgg cttccagcag gcggagattc ttcccgagat cacgtttctg
      541 caccccgagg gtgaagtcgt cgacgtcagt ctggtgctcg atctgggaaa cagccgcgcc
      601 tgcggcattc tcgccgagca ggttcccggc acgccggtga gtctcgacga gtgctgcaag
      661 ctcgagatcc gcgatctgcg cgatccgagt caggtctaca ccgaaccgtt cgataccagt
      721 ttcaagttcc agccacccct ctttcaggaa ccggacaacg cgattcctca ggcaggtggc
      781 gggttcagct ggccgagtct cgtgcgactg ggtcaggaag ccgccaagct cgagccctgc
      841 gatgtcggcg acaccggtat gtcaagcccg aagcgctacc tgtgggacga aaaccagcgg
      901 cacttcccgt ggtatctgaa cctcgccgat gccggtctgg ggaagaagat cagcgcgggg
      961 tttctgaagt ttctcgatga gaacggcgtg tttcgcggtg acaaggcgca gccgcccttc
     1021 gagccctgct acccgccttc ctcgttgatg acctttctga tgatggagat tctcaaccac
     1081 gcgttcgcgc agatcaactc gtacagtttt cgaaaatccc gtgggcaccg gcttgcctgc
     1141 cgggtactga agaatgttgt catcaccacc ccttgcggca tgtcgaaacc cgagcgcgag
     1201 aaataccggc agagagtcca gtcggcgatc gacacggtgt tccatgtcat gcagcttccc
     1261 gcggaacgaa aaccgaaact gtttctggac tttgatgaag cgaccgcgat tcagctgacc
     1321 tatctgttcg gggaggtcaa gcatcgtttt ctcggcgacg cgcgagatgc catcgccacg
     1381 ctcggagccg aacgtcggac aagttccggc gcgcgcgagc ctgtactgcg catcgcgagc
     1441 atcgatatcg gtggtgggac gagtgacctg atgatcgccg agtatgcgca agcttcgagt
     1501 gaggcatccg gagtccgcca acgcatgctg ttttccgaag ggttctcggt ggcgggcgat
     1561 gagatcgcca agc
//
LOCUS       DAORHD010001293         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107052_length_1572_cov_3.674357, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001293 DAORHD010000000
VERSION     DAORHD010001293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107052_length_1572_cov_3.674357"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKO06_20975"
     CDS             complement(<1..677)
                     /locus_tag="PKO06_20975"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNV72194.1"
                     /translation="MNAAMVLERSRALGAGPYERTDQRQGYANGFKPRSFQTRMGDLQ
                     LQIPQVRGISFYPGCLEKGQRSEQALKVAVAEMYLNGVSTRKVTKITEQLCGFEISSS
                     QVSRVAQALDKEVGTFLTRPLGEYSVVYLDARYEKVRHAGHLKNLAVIWAVGIDELGK
                     RQVLGVSCKLSEAEIHWRDFLTDLVTRGLRGVHLIVSDAHQGLRKAREAMFPTVPWQR
                     CMVHLMR"
BASE COUNT          336 a          437 c          387 g          412 t
ORIGIN      
        1 tttcgcatca agtgaaccat gcacctttgc cacggcaccg tcggaaacat ggcctctctt
       61 gcttttcgaa ggccctggtg agcatcactg acaatcagat gaacaccacg aaggccacgg
      121 gttaccagat cagtcagaaa gtctcgccaa tggatctccg cttcgctcaa tttgcaggac
      181 accccgagaa cttgtctctt tcccagttcg tcgatcccaa cggcccagat caccgccagg
      241 tttttgaggt ggcctgcatg tcgaactttt tcataccggg catccaagta aaccaccgaa
      301 tactcaccca gaggccgagt cagaaaggtg ccaacttcct tgtccagagc ttgagcaact
      361 cgcgagacct ggctcgagga aatttcaaaa ccacacaatt gctcggtgat cttcgtcacc
      421 tttcgggtag aaaccccgtt cagatacatc tcagcgacag cgactttcaa agcttgttcg
      481 cttcgctgtc ctttctcaag gcatccgggg taaaacgaga tgccacggac ctgagggatt
      541 tgaagctgaa gatcccccat ccgcgtttgg aacgaacgtg gcttgaaacc gttggcatac
      601 ccttgccgct ggtctgtccg ctcataagga ccggcaccaa gcgccctgga tcgttcaagc
      661 accatggccg cattcatcag aatctccaac actttgggca ttccgtcaaa tccatctcgg
      721 ctgagctgtt cacaaacttc ttcaatcagg ttatccttag cgtcacgggc cattcttctc
      781 ctcctcggtt tttgtcagca ctcaaaccat cggagaaggt ggcccgctct tgcaggtcac
      841 gaatttacag acagaaggtt acacaaccct gtggttcgca ttttttgcag acataactgg
      901 attggaagga tgttgagaaa agtagggtat gctttcgcga tcttcgatgt tggtggcagg
      961 ggtaattcag gtcgttgacc tgttcctcgt ttggactacc tttcagggta tctggggatt
     1021 gagcggtaca ggtttcttac cttctattcc cgactttcgg ggagctgagt gggtgggtcc
     1081 cacgtttctt ctcatctatt atttctcgtt tcggattttc ggattgtacg agttcaacag
     1141 ggttctgtcc tcatctgacc caagccatcg agtattcaga acatcgttgt tgggagggat
     1201 cgtctgtgcc acggtcctgt ttttctcacg gacactgatt tcacgcacat tcatcattac
     1261 tttcatcgtt tcttcagcat tgattctgaa atgtggtcgt ctggttcttt ttggactcct
     1321 tcggaatttg catgtaagtg ctcgcgctcg cagacacatt ctcatcgtcg ggccacgggt
     1381 acgcgcggtg cagttcgtgc aggcgattca acagaaccag ggctggggct acgagatcgt
     1441 cggaatcgtc gataccgagc cggatgccgt gaacacccgc atctcggacg tgccgattct
     1501 cggaacgctc gagaacctcg accacctgct gcgagccgca ccgaccgatg aagtgctggt
     1561 ctgtctgccc cg
//
LOCUS       DAORHD010001294         1572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107079_length_1572_cov_2.812129, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001294 DAORHD010000000
VERSION     DAORHD010001294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107079_length_1572_cov_2.812129"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1222
                     /locus_tag="PKO06_20980"
     CDS             179..1222
                     /locus_tag="PKO06_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72195.1"
                     /translation="MPTVALFTLVFLSFIPWMQTTGNGRYFMPYLLLIGPLCIALINL
                     LQSTANMKVFLTIAVLGLQGFALYQNTPWRPVDSWTWIEWKDAPYLPLEFAKEAIDPE
                     AIYVTIAVPTYSLAAPLLPASSRWINISTFGSSDKDKQSALYAPVKKALLSGKPLNLF
                     MVSAPRSMKDGSVQPDQNAINQITPYLEAHDLRLKSPTNCQLIKSKSMAPTGFIVTEE
                     SPAARERVIEQSGFWICPIEYAVSPRQSNALTAEQLGAKALFEKMEQICPRFFNPGQQ
                     GVSYHRSGFQRRYSVSDSFLIATGDGHLYFKYDRTLNPQLIGTAEDVLSPGFSFDCTK
                     FKGRAGLPWEREI"
     gene            1219..>1572
                     /locus_tag="PKO06_20985"
     CDS             1219..>1572
                     /locus_tag="PKO06_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72196.1"
                     /translation="MSLAGRLFMDVSYTRTQHGNVGITRTVRRLLDELETIAGCKPVV
                     FHRTGFRLLRPAHRSPTASGVTAVDKAAGRMFRWLNSASVRKLVSLIPEAVLHRAWRL
                     NNDLTFDSLSRDEEPL"
BASE COUNT          321 a          461 c          416 g          374 t
ORIGIN      
        1 tcgattcctg gccgacacct ggcgcgacgc cattctgcgg cccgtggcta tggccgcacc
       61 caacagttcg gtttacatcg agatcgccgc acccgatctc cggtttgcta ttgcacttgt
      121 tctggcagtg ttggtcttga tttccacccg atagaccaag ctccagccgc agggctggtt
      181 gccgacagtg gctcttttta ctctggtatt cctgtcgttc ataccctgga tgcagacgac
      241 aggcaatggc cggtatttca tgccgtatct gctattgatc gggccgttgt gcattgcgct
      301 catcaacttg ctgcaaagta ccgcgaacat gaaagtgttc ctgacgattg cggttctcgg
      361 attgcaaggc ttcgcgctgt accagaacac gccttggcga cccgtggact cctggacatg
      421 gattgagtgg aaagatgcgc cttatttgcc cctcgaattc gcgaaagaag ccattgaccc
      481 ggaagccata tacgtgacca tcgcggttcc gacctattct ttggccgcgc cgctgctccc
      541 cgcctcttcg cgctggatca acatctcgac atttggctcg tcggacaagg acaagcagtc
      601 tgccctttat gccccggtca aaaaagcgct tctgagcggc aagcccctga atctgttcat
      661 ggtcagcgcg ccgcgatcca tgaaggatgg ctccgttcag cccgatcaga acgccatcaa
      721 tcaaatcact ccgtaccttg aagcccatga tcttcgcttg aagtcaccca ccaattgcca
      781 gttgatcaaa tcaaagtcga tggcgccaac cggttttatc gtgactgaag agagccccgc
      841 cgcgagagag cgggttattg aacaaagcgg gttctggatt tgtccgatcg aatacgccgt
      901 ttctccccgg cagtcgaatg cattgactgc cgagcagctc ggtgccaagg cactgtttga
      961 gaagatggag cagatctgtc cgcgcttttt caaccccggg caacagggtg tcagttatca
     1021 ccgttcgggt tttcaaaggc gttatagcgt cagtgattcc ttcctgatcg caacgggcga
     1081 cggccatctc tatttcaaat acgatcgaac gctgaacccc caattgatcg ggacggccga
     1141 agatgttctt tctccgggct tcagctttga ctgcaccaaa ttcaaaggcc gcgccggcct
     1201 tccgtgggaa agagagatat gagtcttgcg ggccggttgt tcatggatgt cagttacacc
     1261 cgaacccagc acggcaatgt cggcatcacc cgcaccgttc gccggttgct ggacgaactt
     1321 gagaccatcg ccggctgcaa gcccgttgtt tttcatcgca ccgggtttcg gcttctccga
     1381 ccggcccatc gttcgcccac ggcatccggt gtgacggcgg ttgacaaggc cgccggccgc
     1441 atgttcaggt ggttgaactc cgcatctgtt cgaaaacttg tttcactgat tccagaggct
     1501 gtcttgcaca gagcctggag actgaacaac gacctgacgt tcgattcact gagcagggat
     1561 gaagaaccct tg
//
LOCUS       DAORHD010001295         1569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107377_length_1569_cov_2.794584, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001295 DAORHD010000000
VERSION     DAORHD010001295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107377_length_1569_cov_2.794584"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKO06_20990"
     CDS             <1..210
                     /locus_tag="PKO06_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72197.1"
                     /translation="VLVLTYLGGVEDSLAASIDGEVNTGICDVVVTVPTHQQQAFASV
                     SQVFRYGFERLAQQFPTFIIIERIS"
     gene            227..496
                     /gene="rpmA"
                     /locus_tag="PKO06_20995"
     CDS             227..496
                     /gene="rpmA"
                     /locus_tag="PKO06_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNV72198.1"
                     /translation="MATKKGGGSSVNGRDSNPKHLGPKKFDGEVVRAGNVLVRQRGTP
                     IHAGRNVGMGKDDTLFALIDGKVKFATRKNRHWVDVLPLTTETAK"
     gene            636..788
                     /locus_tag="PKO06_21000"
     CDS             636..788
                     /locus_tag="PKO06_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72199.1"
                     /translation="MSVQELLALYRCQDHPTCPICRKIVLRYERSLNLSDADRLHMQK
                     CILQNP"
     gene            complement(861..1394)
                     /locus_tag="PKO06_21005"
     CDS             complement(861..1394)
                     /locus_tag="PKO06_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72200.1"
                     /translation="MRKLLTVVGLAVALVAGCPMGVQAAKADKEAKKAVVAKESVEKT
                     GVIEVVKADAAKKEKYDTVLLKVGEESFKLIPGKNKKVFKMLEVMAGKNVTVKGTLMP
                     ANPPKYPLAAIKVDEYTESTAAPSAAPAPATPAPAADAPAPAADAPAPAPAADTAAPA
                     TDAPAADAPAPAPTEGN"
BASE COUNT          336 a          435 c          420 g          378 t
ORIGIN      
        1 gttctcgtcc tgacgtatct gggtggagtt gaagactcgc ttgccgccag tatcgacggc
       61 gaggtgaaca ccggaatctg cgatgttgtt gtcacagttc cgacgcacca gcagcaggct
      121 ttcgccagtg tatcgcaggt ttttcggtac gggtttgaac ggttggcgca acagtttcca
      181 acctttatca taatcgaacg tatttcataa gaaggagtcg ggcttcatgg ctaccaaaaa
      241 aggtggtggg tcatcagtaa acggtcggga tagcaacccg aaacaccttg gccccaagaa
      301 atttgatggt gaagtggttc gggccggcaa cgtcctggtt cgccagcgcg gcacgcccat
      361 tcacgccgga cgcaacgtcg gcatgggaaa agacgacacg ctgttcgctc tgatcgatgg
      421 caaagtcaaa ttcgccacgc gcaagaatcg tcattgggtc gatgttctgc cgctgacgac
      481 cgaaaccgcg aagtaacgtt ttttcggtga atcaacccct gctgcggcag gggtttttgc
      541 ttgtacccca atcttttatg gcgacctctc agccaacggg agcaaaatgc cgatagtatt
      601 cctagaggtt cattccacgg aaggaggcat ttgtcatgtc agttcaggaa ctgctggctc
      661 tgtatcgatg tcaggatcac cccacctgcc ccatctgtcg aaagatcgtc ctccgctatg
      721 aacgatctct caacctgagt gacgctgatc gcctccacat gcagaagtgc attctccaga
      781 acccgtgatc tgagtaaaca gccgatcgta cagaaaaaac caggccccaa agggcctggt
      841 tttttctttg cgttttttgt ttaattgcct tcggtcgggg caggggccgg agcatccgct
      901 gcgggagcat cggtggcagg agctgccgtg tcggctgccg gagctggagc cggagcgtca
      961 gcggcgggtg ctggggcatc agcggcagga gccggggtag cgggcgcagg agcggcagaa
     1021 ggagctgccg ttgactcggt gtactcgtca actttgatcg cagccagagg atattttggc
     1081 ggattggcgg gcatcaaggt tcccttcacg gtcacatttt ttccggccat gacttcgagc
     1141 attttgaaca cttttttgtt cttgccagga atcagcttga atgattcttc tccaaccttg
     1201 agcagaaccg tgtcatactt ttctttcttc gcggcatcag ccttgaccac ttcaatgaca
     1261 cctgtcttct cgacgctctc tttggctacg acagccttct tggcctcttt gtccgctttc
     1321 gcggcctgta cgcccatggg acaaccagca accaaagcca ctgccagacc gacaaccgtc
     1381 agaagtttcc gcataacgac ctccgtcttg cacctgatga atgtacccgc atcaacgcgg
     1441 gtagagaagc atatactcct gctgtgcgca cctgtcaatt tatttccgct gcggcaacgc
     1501 ctgtctagtc tgcaaaagca cacgaacgtt gtcgataaac cctcttccca cggccgtttt
     1561 gacccacga
//
LOCUS       DAORHD010001296         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107458_length_1568_cov_3.753470, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001296 DAORHD010000000
VERSION     DAORHD010001296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107458_length_1568_cov_3.753470"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="PKO06_21010"
     CDS             <1..809
                     /locus_tag="PKO06_21010"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV72201.1"
                     /translation="VSIELAAQTVLGAAKTVQTSRTRSSLNTTRHQMLNHRVDIESVL
                     ACVGTTTLDISIPATPTFELASMTLAAATDLMLARDFDLALIANDAAIFRKGIHAARQ
                     ANLPTLDINSNYTYGHKVGTMKDTRDYTVTFGLSFPVYDGGDIRNEVRKAEKTLAELE
                     LAHEDLVNRKRALLTTYFNDYHEAVERIAVAAKAMEQSRENMRMVMTRYEHGAASIVE
                     LVDAQLTLSSTSEEAVDARIDERLRLADILLAIHDLEALCTLDKVPGDTP"
     gene            806..>1568
                     /locus_tag="PKO06_21015"
     CDS             806..>1568
                     /locus_tag="PKO06_21015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV72202.1"
                     /translation="MKHSLIAVCLVLLMASSLSAAVTVVTQAVTTGDICERLAFSGEV
                     KPFQEAIVSPDVSGMVEKVLVENGQPVKARQPLILLDRSRAVIALHQAEIQVKKAEQQ
                     VKESRSDFERNRILREKQVLNERAFEISESQFVNANNSLREASAGVELARLNLDRAAI
                     TAPFAGFFVNRQVFPGQWLTTGQPLGRVIDLTQAYIEARIPETHINRLRVGQECQIAG
                     NTPGKVSFIDLYADSSRGFLIKILTGNPELRFKANM"
BASE COUNT          343 a          493 c          419 g          313 t
ORIGIN      
        1 aggtctctat tgagctagca gcgcagactg tgctcggcgc ggctaagaca gtgcagacct
       61 cacgcacgcg cagttctctg aacacgacca gacaccagat gctcaaccat cgcgtcgata
      121 tcgaaagtgt cctggcctgt gtcggcacga ccactcttga catcagcatt ccggcaaccc
      181 cgactttcga actcgcgtcg atgaccctcg cggcagccac ggatctgatg ctggctcgcg
      241 acttcgacct ggctctcatc gccaacgatg ccgccatttt tcgtaaaggt attcacgcgg
      301 cgcgacaggc aaacctgccc accctggaca tcaacagcaa ctatacgtac gggcacaagg
      361 ttggaaccat gaaggatacg cgtgactata ccgtgacctt cggtctctcg ttcccggtgt
      421 atgacggtgg cgacattcgc aacgaggttc gcaaagccga aaagaccctg gccgagctcg
      481 aactggcaca cgaggacctg gtcaatcgca aacgcgctct gctgaccacc tatttcaacg
      541 actatcatga ggcggtcgag cgcatcgccg ttgccgcgaa agccatggag caaagtcgcg
      601 agaacatgcg catggtcatg acccgctacg agcatggcgc ggcctccatt gtcgagcttg
      661 tcgatgccca gctcaccctg tcgtcgacct cggaagaagc agtcgatgcc cgcattgatg
      721 aacgtctgcg tctcgctgac attctgctgg ccattcacga cctcgaagcc ctgtgcacat
      781 tggacaaggt accaggagat accccgtgaa acactctctc attgccgtct gtctcgttct
      841 tctcatggca tcctcgctct ctgctgccgt caccgtcgtc acgcaggccg tcaccacggg
      901 ggacatctgt gagcgcttgg ccttttccgg tgaagtcaaa cccttccagg aagcaatcgt
      961 ttctccggat gtttcgggaa tggtggagaa agtcctggtc gaaaacggac agcccgtcaa
     1021 agcccgacag cccctgattc tgcttgatcg ttcgcgcgcc gtgatcgctc tgcatcaggc
     1081 cgagattcag gtcaagaagg cagaacagca ggtcaaagag tcacgcagcg atttcgagcg
     1141 caatcgcatt cttcgcgaaa aacaggtgct caacgagcgc gccttcgaga tctcggaaag
     1201 ccagttcgtg aatgccaata acagcctgcg cgaggcatca gccggcgtgg aactggcacg
     1261 tttgaatctc gaccgcgcag cgatcaccgc cccctttgcc gggttcttcg tcaatcgcca
     1321 ggtttttccg gggcagtggc tgacgactgg ccaacccctc ggacgagtca tcgatctgac
     1381 gcaggcttac atcgaagccc gcattccgga aacccacatc aaccgtctgc gcgtcggtca
     1441 ggaatgccag atcgccggca acacgccggg caaggtctcg ttcatcgacc tgtatgccga
     1501 ctcctcgcgg ggatttctga tcaagatcct gaccgggaac cccgaactgc gattcaaggc
     1561 gaacatgt
//
LOCUS       DAORHD010001297         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107470_length_1568_cov_3.405155, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001297 DAORHD010000000
VERSION     DAORHD010001297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107470_length_1568_cov_3.405155"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..>1568
                     /locus_tag="PKO06_21020"
     CDS             79..>1568
                     /locus_tag="PKO06_21020"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNV72203.1"
                     /translation="MKRFAAFGRLARMMRFSASIPNRVRRLILLTLDTLSLTFALLAA
                     IWLRFEQLELRHASYFLTIWVAIIPLSLLVFSFMGLYRRSVRFTSISDLVAIFIAVTT
                     TTTLKLGTLYFHHGLGYSRSIVIIDWFLSLIFIGFSRLLPRLLLSFAEYETVRAIVFG
                     KARNPAKRVLIFGAGHAGENVVREILRNENFPYRIVGFLDDSPRKLGSVIHGIEVLGT
                     RQDLNRVVRERGVNEIIIAIPSASGDAIREIVHLCRDTKVKFLTLPGMQEILEGKLLS
                     MQLRDISVEDLLRRAPADINLAEIAAYITGKTVLVTGAGGSIGSEICRQILPFQPKIL
                     MVLGKGENSIYQIHQELLESRQLGQITVLPVIADVRDEDRLKKLFHEHRPQIIFHAAA
                     HKHVPLMEANPDEAAKNNVFGTANLVRLAHEFRAERFVMISTDKAVNPTSVMGATKRA
                     AEMILKMHAKKSATRFCAVRFGNVLGSRGSVIPLFKKQISRGGPIT"
BASE COUNT          313 a          471 c          418 g          366 t
ORIGIN      
        1 caacttcgaa accttcgccc gctccgtcgt tcgcgcaatg atgaaataat cggacgtgcg
       61 tgatataatc gcccctcgat gaaacgtttt gcggctttcg ggcgtctggc ccgcatgatg
      121 cgtttttcgg cgtcgatccc caaccgggtt cggcgcttga ttcttttaac gctcgatacc
      181 ctgagcctga cctttgctct actggccgcg atctggctga ggttcgaaca actcgaactg
      241 cgtcacgcca gctactttct aaccatctgg gtcgcgatca tccccctgtc gttgctggtg
      301 ttcagcttca tgggtcttta ccggcgttcg gtgcgcttca cctcgatctc cgacctggtc
      361 gcgatcttca ttgccgtcac gacgacgaca acgctgaaac tgggcaccct gtatttccat
      421 cacggtctcg ggtattcgcg atcgatcgtc atcatcgact ggttcctgag tctgatcttc
      481 atcgggttct cgcgcctctt gccgcgatta ctcctgagtt ttgctgaata cgagacggta
      541 cgcgccatcg tcttcggcaa ggcccgcaac ccggcaaaac gggtgctgat cttcggcgcc
      601 ggccacgcgg gggaaaacgt ggttcgcgag attctgcgca atgaaaactt cccgtatcgc
      661 atcgtcgggt tcctcgatga ttctccacgg aagctgggca gtgtcatcca tggcatcgaa
      721 gttctgggca cccggcaaga tctgaatcgc gtcgtcaggg aacgcggtgt gaacgagatc
      781 atcattgcta ttccttcggc gtcgggtgat gccattcgcg aaatcgtgca tctctgccgc
      841 gataccaaag tcaagtttct caccctgccc ggcatgcagg aaattctcga aggcaagcta
      901 ctcagcatgc agctgcgcga catctcggtc gaagacctct tgcgccgcgc tccggctgat
      961 atcaacctgg ccgagattgc cgcgtatatt accgggaaga cggttctcgt gaccggcgcg
     1021 ggtggcagta tcggcagtga gatctgtcgc cagattcttc cgtttcagcc caagattctg
     1081 atggtgctcg gcaaaggtga aaacagcatc tatcagatcc atcaggaact gctcgagtcg
     1141 cggcaactcg gacaaatcac cgttcttccg gtcattgccg atgtgcgtga cgaagaccgg
     1201 ctcaagaagc tgtttcatga gcatcgcccg cagatcatct tccatgccgc cgcccacaaa
     1261 catgtgccac tcatggaagc caaccccgac gaagccgcca agaacaacgt tttcggcacc
     1321 gccaacctgg tacgattggc tcatgagttc agagccgaac ggttcgtcat gatttcgacc
     1381 gataaggccg tgaatccgac ttcggtcatg ggtgccacca agcgtgccgc cgagatgatt
     1441 ctcaagatgc acgccaagaa gagcgcgacg cgtttctgcg ctgtgcgttt cggcaacgtc
     1501 ctcggcagtc gcggcagtgt catcccgctg tttaaaaagc agatatcccg tggcggcccg
     1561 attaccat
//
LOCUS       DAORHD010001298         1568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107513_length_1568_cov_2.707204, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001298 DAORHD010000000
VERSION     DAORHD010001298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107513_length_1568_cov_2.707204"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PKO06_21025"
     CDS             complement(<1..1136)
                     /locus_tag="PKO06_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72204.1"
                     /translation="MLRWSLAVVLVCCTSTAYADLPAFISNNTTDTNLNSIAIIGETI
                     TYQCTTLATPAPLLGEVQVFPGGPVLNTAAFANLGSNIYRYQITFGPITEGLQNGVQS
                     PAQFFITNASGTLTCAPVSLVTLDNRPPARAGPFDMTVNGLPYTGQILHRGDVLGFSQ
                     LMDGTAFQAGETATLNMSAIGLGNVTLTTANPHTGNYTISAIVDAPPGNTTGDLTLQM
                     VDPYGNATSATVLTGVTIDSRAPVISSTNVIVTPAGLARPGSQVTIQVNIAPNPYDND
                     TVVASCTYLGGTSLPLPWNGIRFEGTFTVASSPPASAIVGSTFAEITMTDNGFNVATA
                     TTPTFPIDNRLPLLDPQPNAQIIQSNGVLADAIAIVGDRLTIAAT"
BASE COUNT          384 a          391 c          447 g          346 t
ORIGIN      
        1 gtagcggcga tcgtcagacg gtcaccgaca atggcaatgg cgtcggcaag tactccgttg
       61 ctctgaatga tctgcgcatt cggctgtgga tcaagtaacg gcaatcggtt gtcgatgggg
      121 aatgtcggcg tcgtggcggt tgccacatta aagccgttgt ctgtcatcgt aatttcagca
      181 aaagttgaac ctacgatagc tgatgccggg ggactcgagg caaccgtgaa agttccctcg
      241 aatctaatcc cattccatgg gagaggaaga ctggtaccac caaggtaggt gcaggaagcc
      301 acaaccgtat cattgtcata aggattcggg gcaatattga cctgaatcgt aacttggctt
      361 cccggtctcg ccaggccagc aggagtaaca atcacattcg tcgaggagat gactggtgct
      421 ctcgaatcaa tcgtcactcc agtcagaacc gtcgccgacg tagcatttcc atacggatca
      481 accatttgta gagtcaggtc gccggtagtg tttccaggag gagcatcaac aattgccgaa
      541 atcgtatagt ttcccgtgtg gggattcgcg gttgtcaaag tgacattgcc caaccctatc
      601 gcactcatat tcaaggttgc ggtttcgccg gcctgaaagg ctgttccatc catcagttgg
      661 ctgaatccaa gaacatcgcc acgatgcaag atttgtccgg tgtagggaag cccgttcact
      721 gtcatgtcga atggtccagc tcgagcggga ggtcgattgt caagagtaac gagcgaaacc
      781 ggagcacacg tgagcgttcc gctcgcattt gtaataaaaa actgagctgg actctggact
      841 ccattctgca atccctcagt gatcggacca aacgtgatct ggtagcgata aatattcgac
      901 ccaaggtttg caaatgccgc agtgttcaat accgggccac cgggaaagac ctggacttct
      961 cctagaaggg gagcgggtgt ggccagcgtg gtgcactggt aggtgatggt ctcgccaatg
     1021 atagcaatgc tgttgaggtt ggtatccgtc gtattgttcg aaatgaacgc tggaagatcg
     1081 gcatacgcgg tcgaggtgca acagaccagc accacagcaa gcgaccagcg cagcatggtg
     1141 gcgacgagct tccagggcgc gaatggagag ggcgaagcct tgcggactcg aggatatcta
     1201 tacatcacag acgatttccc ccaaatagcg ctatcggtag acgacggtct attcatcgtt
     1261 catcggcatt ctggggtcag aaggtgagca gttgttgact cttcaagtca gagaacgggg
     1321 ttcgcgatcg tctcccgcgg ggatacagag aaacgggaac tgctacggtg aaatcacacc
     1381 atcgggtgtg gcaaccggtt caacctctct gcagccaggg aaaaccaggc acagccaaga
     1441 gtcgaacgca atcaggcgct ggtcgagacc tcggcaggct tttgccggtc ttcagcgctg
     1501 actttcttgc gcatgatcac gcgtgtgctc ttgccaggat gaaagacgaa atagacttca
     1561 tccatgta
//
LOCUS       DAORHD010001299         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107604_length_1567_cov_3.907407, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001299 DAORHD010000000
VERSION     DAORHD010001299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107604_length_1567_cov_3.907407"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKO06_21030"
     CDS             <1..920
                     /locus_tag="PKO06_21030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose PTS transporter subunit EIIB"
                     /protein_id="HNV72205.1"
                     /translation="LTGITEPIEFSFMFVAPALYALHAVLASVAFPVCILLGIKHGTS
                     FSHGLIDYIVLFPKSTHALWFLVIGPLWGVLYFFLFRFVINAWDLKTPGREEEEGPVV
                     EGGSPVAAEFAHQLVLGFGGRSNIRSLDACITRLRVEVADMSKTNPDKLKALGASGVV
                     VVGNGLQAIFGTRSENLKTDMEEYLATAGAEAELSDSDLVASHAVSAPAGVPSKQRDP
                     QAAEKARAIVAALGGAANISRCEACAETRLRIEMKQSQKASTETLREVGVAAVMPQPN
                     GAWHLLVGLNADQYAIEVQALIVQSAKVV"
     gene            1013..>1567
                     /locus_tag="PKO06_21035"
     CDS             1013..>1567
                     /locus_tag="PKO06_21035"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HNV72206.1"
                     /translation="MWKKLVISVVVLCVGTSLLCCGYVWNYRRQVEAYARAERRSLQF
                     GPLTRTYELFVPKNVVGSSPVPLLLVLHGGGGTGRGMQYLTLGRFNELAEREGFVVAY
                     PDGLKKNWNDGRDVPASEAHASKLDDVGFLKALIQKLQTELSIDPSRIYACGISNGAF
                     MSARLAIEAPDVFAAVALVTGSLPT"
BASE COUNT          286 a          419 c          513 g          349 t
ORIGIN      
        1 tcctgaccgg tatcaccgag cctatcgagt tttctttcat gtttgtcgct cccgccctct
       61 atgcccttca tgccgtgctg gcttcggtgg ctttcccggt gtgcattctg ctggggatca
      121 agcatggcac gagcttctcg catgggttga tcgattacat cgtcctcttt cccaagtcca
      181 ctcacgccct gtggtttctg gtgatcggcc cgctctgggg cgtgctctac ttctttctgt
      241 ttcggttcgt tatcaacgcc tgggatctca aaaccccggg ccgagaggag gaagaagggc
      301 cggtagtcga ggggggcagt ccagtggcgg ccgagttcgc acaccagctg gtacttggtt
      361 tcggcgggcg cagcaacatt cgcagtctcg atgcctgcat cacgcgcttg cgggtcgagg
      421 tcgccgatat gagtaaaacc aacccggaca aactgaaagc cctgggtgcg agtggagtcg
      481 tcgtggtcgg caacggtctg caggccatct tcggtacccg ctccgaaaac ctgaaaaccg
      541 acatggaaga gtatctcgcg acggcaggtg cggaagcgga attgtccgat tcggacttgg
      601 tcgctagtca tgcggtcagc gcacccgcgg gtgtgccgag caaacagcgt gacccgcagg
      661 cggcggaaaa ggcgcgggcg attgtcgctg cgctcggagg tgctgcgaat atcagccggt
      721 gtgaggcgtg tgctgagact cgtctgcgta tcgagatgaa gcagtcgcag aaagcctcga
      781 ccgaaaccct gcgtgaggtt ggagtggctg ctgtcatgcc gcaaccgaac ggtgcctggc
      841 atctgctggt cggactcaat gccgatcaat atgccattga ggttcaggcc ctgattgtcc
      901 agtctgcgaa agtcgtatga gagcatcgat ggcggatcgg gcgacctgac agattgctcg
      961 caggttggct gtcgatggga agtcgctggg tgtgacctga gaggtggacc gcatgtggaa
     1021 aaagctggtt atcagtgtcg ttgtgctctg tgtcgggacg agcctgctgt gttgcgggta
     1081 cgtttggaac taccgcagac aggttgaagc ctatgcccgg gcggaacggc gctccctgca
     1141 atttgggccg ttgacgcgaa cgtacgagtt gtttgtcccc aagaatgtcg tgggaagctc
     1201 gcctgtgccg ctgttactgg ttctgcacgg cggtggggga accggccgcg gcatgcagta
     1261 tctgaccctc ggacggttca acgaactggc agagcgtgaa ggctttgtgg tcgcctaccc
     1321 ggatgggctg aagaagaact ggaatgacgg ccgtgatgtt cccgcctcag aagcacatgc
     1381 ctcgaaactc gacgatgtgg gattcctgaa ggcgctgatt cagaagctgc agacggaact
     1441 ctcgattgat ccttcccgaa tctatgcctg tgggatctca aacggggcgt tcatgtcggc
     1501 tcgcctggcg atcgaagcgc ctgatgtctt cgcggcggtg gcactggtga ccgggtcgtt
     1561 gccgacg
//
LOCUS       DAORHD010001300         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107608_length_1567_cov_3.736111, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001300 DAORHD010000000
VERSION     DAORHD010001300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107608_length_1567_cov_3.736111"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKO06_21040"
     CDS             complement(<1..279)
                     /locus_tag="PKO06_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72207.1"
                     /translation="MLIRSKNRLYHFDRHISDSLLRLCMFALFLFCLVSGYLCLSRTE
                     PLIDSTFWPRLQDEIADIAGKDVSKLGVSEKSLLTTALHEMERDKVGIT"
     gene            321..863
                     /locus_tag="PKO06_21045"
     CDS             321..863
                     /locus_tag="PKO06_21045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72208.1"
                     /translation="MQAEKDHKTAIRAANGIRFEVAAGAGLGSRTDHLIPTDVTDNRS
                     FCPVKMTSAIDAAQQIESICQIQSSFYRNVRDEKYQGKNEGEWQQKQAKNESNIYFLA
                     KQKVEIRVHIPVVQFDVILNDSRQQDQGNEREGNPNGKSGSGPSGGMNGAGRNDQLEE
                     KTKIVADGRELEILKGLPFE"
BASE COUNT          413 a          393 c          400 g          361 t
ORIGIN      
        1 agtaatgccg actttgtcgc gctccatctc atgcagagcc gtcgtcagca ggctcttttc
       61 cgaaacaccg agcttgctga catccttccc cgcaatgtct gcaatttcat cctggaggcg
      121 tggccagaag gtcgaatcaa tcagtggttc ggtacgggag agacagaggt aaccggagac
      181 cagacagaac aggaacaaag caaacatgca caatcgcaag agcgaatcgg agatatgccg
      241 atcgaagtga tacagtctat tctttgatct gatcagcatg agccgtctcg cttcccggcc
      301 ttcgttgact ggtgctctcc gtgcaggccg agaaagacca caaaaccgca atacgggcag
      361 cgaacggcat tcggtttgaa gtggcagccg gggcaggtct gggtagccga accgaccacc
      421 ttatccccac agatgtgaca gataaccgtt ccttctgccc agtgaagatg acttccgcaa
      481 ttgacgcagc gcagcaaatc gagtcgatat gccagatcca aagcagtttt taccgcaatg
      541 ttcgtgacga aaaatatcaa ggaaaaaacg agggcgaatg gcaacagaag caggcgaaaa
      601 atgagtcgaa tatctacttt ctcgcgaaac agaaagtaga aatccgagtt cacataccag
      661 ttgtacagtt tgatgtaatc ctcaacgact cgcggcaaca ggatcaggga aatgaacgcg
      721 aaggcaaccc caatggcaag agcggcagcg gcccaagcgg cggcatgaat ggcgccggaa
      781 gaaatgatca gcttgaagag aaaaccaaaa tagtagccga tggacgagaa ctcgagatac
      841 tcaagggttt gccattcgag taatctaccc atccggtttc tcctgaaaca gcaagtcagc
      901 aggaaacaga aagattgcgg tcagaccaaa tcggacgagg gctatcaatt gctgacacct
      961 tcccctcttc atgcttagtg tgaaagtcta gtgtgaatgc gagaaaagtc aaggcaaaaa
     1021 ggaagttcat gtcagagcgg gcgctgtcca gccgattctg ctgtttcagt tggattcacg
     1081 gaaaagtgaa ggcgatgcgc tgacagggcg tggaactcat ctcgcctttt ctcgatggtg
     1141 tttttacgct acactgcctt ccatgaaacc gaaacccttt gccaggacga ggatagcccg
     1201 aagagttttt gtggcctttc ttctggcctc ctttgccctg tcacagatcc atctatttct
     1261 cggcagagac gatggtttct tcgtcgttca tcgtgaatcc catgttgaac tgacggttcc
     1321 catacaggat cctgcgtcct tcctgcttgc gttttttctg ctgcatgttc cttcaagccc
     1381 tggtctcgaa gaggtttttc atgacgagag tctggtctgt gacaggagat tgtctcctct
     1441 tcgagtgacg gttcaaagcc cacacgtttt gcctgaagtc aggattctgg ctatggaatc
     1501 gctcagggta tcccgctttg atgatgccag taaaagtgat ccacctcaaa agcgtcgcat
     1561 ttttctc
//
LOCUS       DAORHD010001301         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107621_length_1567_cov_3.235450, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001301 DAORHD010000000
VERSION     DAORHD010001301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107621_length_1567_cov_3.235450"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PKO06_21050"
     CDS             <1..826
                     /locus_tag="PKO06_21050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72209.1"
                     /translation="GNLGKMIGQIESAEDLLAALEATRTYGSGDAAKAYTSGVAVKML
                     KERAAFLKVELPADQSVDSALTQITLETTPQVNDVRTLLTNSQRALIMVQTPVAEAVI
                     SFEDGKEQRRLNAFKAMIDRHECRVISSAVDKSGEQPRYNFYLSGKKFVLEAMIRHYG
                     GQEVNSNLKAVLVLTGGGFWGQKTVEVTVNPTSQEKGTLAWYQAVLEKDPYKYVAETQ
                     YETIAQLGKVETLAGENKLRIKNAKMELWVMPANGTRRDVIYYNSAEYGDIYVSAR"
     gene            853..>1567
                     /locus_tag="PKO06_21055"
     CDS             853..>1567
                     /locus_tag="PKO06_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72210.1"
                     /translation="MLNQIKKYISVSAVALALLMPVSTQAATEAEIEQLEKQLIQKES
                     QLLEPELKLDKLVVERAKLDGMGGWFQGSKKKELDRQIAEAESSINRTYSEMKGLQKQ
                     VQDTVFAVAQTYEGKGQYLKAIEYYLKIENQDDRIRFRIATCYKQAQDYPSAIQWFLK
                     LSRTDQNLLEVVDCYKLAGNLKDTFYWLFQILEPIDLNAAEVAALELIEKYDYPAKKI
                     DYPNFDQRLSDVYLSKAVRE"
BASE COUNT          403 a          462 c          432 g          270 t
ORIGIN      
        1 gggcaacctg ggcaagatga tcggtcagat cgaatcggcc gaagatctgc tggccgctct
       61 cgaagccacc cgcacttatg gttccggtga cgcagcgaaa gcgtatacgt cgggcgtagc
      121 cgtgaagatg ctgaaagagc gcgcggcctt cctgaaggtc gaactgcccg ccgaccagag
      181 cgtcgactca gcgctcaccc agatcaccct cgaaaccacc ccccaggtca atgacgtgcg
      241 cacactgctg accaactctc agcgcgccct gatcatggtt cagactccgg tcgccgaagc
      301 agtcatctct ttcgaagacg gaaaggaaca gcgccgtctg aatgcgttca aggccatgat
      361 cgaccgtcac gaatgccggg tgatttccag tgccgtcgat aagtctggtg aacagccccg
      421 ctacaatttc tacctcagtg gcaagaagtt cgtgctcgaa gccatgattc gccactacgg
      481 cggccaggaa gtgaacagca acctgaaggc ggtactcgtg ctcacaggcg gcggcttctg
      541 gggccagaag accgttgaag tgaccgtgaa ccccaccagc caggaaaagg gaacgctggc
      601 ctggtatcag gcggttctcg aaaaagaccc gtacaagtac gtggcggaaa cccagtatga
      661 aacgatcgcc cagctcggca aggtcgaaac cctggccggc gagaacaagc tgcgcatcaa
      721 gaacgcgaag atggaactgt gggtcatgcc ggccaacggc acccgccgcg atgtgatcta
      781 ttacaacagc gcggaatacg gtgacatcta cgtgagcgcc cggtaagcga agagagaaag
      841 gagcacaaaa cgatgttgaa tcagatcaag aaatatatct ccgtcagcgc cgtcgccctg
      901 gctcttctga tgccggtctc cacacaggcc gccaccgaag ccgagatcga acagctcgaa
      961 aaacagctga ttcagaaaga atcgcagctg ctcgagccgg aactgaaact ggacaaactg
     1021 gtggtcgaac gcgccaagct cgacggtatg ggtggctggt tccagggttc aaagaagaag
     1081 gaactcgacc gccagatcgc cgaagccgag tcctcgatca atcgcactta cagcgagatg
     1141 aaaggcctgc agaaacaggt tcaggatacg gtcttcgccg tggcccagac gtacgaaggc
     1201 aaaggccagt atctcaaggc catcgagtac tatctcaaga tcgagaacca ggatgatcgc
     1261 atccgcttcc gcatcgccac ctgctacaag caggcgcagg actacccttc cgcgattcag
     1321 tggttcctga aactgtctcg caccgatcag aacctgctcg aagtggtcga ctgctacaaa
     1381 ctggccggca acctgaaaga caccttctac tggctgttcc agattctcga acccatcgat
     1441 ctgaacgccg ccgaagtggc cgccctcgaa ctgatcgaga agtatgacta ccccgccaag
     1501 aagatcgatt atccgaactt cgatcaacgc ctttccgatg tgtatctgag caaggccgtg
     1561 cgcgagt
//
LOCUS       DAORHD010001302         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107862_length_1565_cov_3.217219, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001302 DAORHD010000000
VERSION     DAORHD010001302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107862_length_1565_cov_3.217219"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKO06_21060"
     CDS             <1..242
                     /locus_tag="PKO06_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72211.1"
                     /translation="KSERTSILTVPYEMIPLPKVGDIVIGLDRSGKPVCEVKVLRVGG
                     CKKHDRTSAITFELPREFDMDVRAIRVKSGKGAGK"
     gene            239..580
                     /locus_tag="PKO06_21065"
     CDS             239..580
                     /locus_tag="PKO06_21065"
                     /inference="COORDINATES: protein motif:HMM:NF037640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNV72212.1"
                     /translation="MKIVCRCQDITEEEVLSAIRQGATTLDEIKRLVRAGMGPCQGRT
                     CRRLVQQIISRELKKPMAEIHPSTFRPPNLPVPFNLVMAEYEKIEKEKERKTVRKPSA
                     KGKGKAKEGDA"
     gene            577..>1565
                     /locus_tag="PKO06_21070"
     CDS             577..>1565
                     /locus_tag="PKO06_21070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV72213.1"
                     /translation="MKTSYDAIVIGGGILGVSIAYELARAGMKNICVLEKHYLASGST
                     GRCGGGFRQQWSTEHNTQLAMDSVKRLEGLEQELEYNCEMFQGGYLILAHTDADVAQF
                     EKNVAMQRRLGLDVNMVDPAEAKKIVPHLDTSLIKKATWCPKDGHINPFLLTQGYANA
                     AKRLGVDVHLWTDVTSIMKKSNVFFVMTNTGFTYQAPIVFNCAGGFAREIGKMLNVDI
                     PVDPYRHEILVTEPVERLWNPMVISFTVGLYARQEANGGVVMGCGDPNEPVGTYVGSS
                     MHFMYDMSKRFTTLFPKLGNLRIVRQWAGLYEMTADAQPILGPVDEVENFYQA"
BASE COUNT          354 a          411 c          482 g          318 t
ORIGIN      
        1 ccaagtccga gcgcacatcg attttgacgg tcccctacga gatgattccg ttgcccaagg
       61 tcggcgacat cgtcatcggg cttgatcgca gcggcaaacc agtctgcgag gtgaaggtgc
      121 tgcgggttgg tggttgcaag aagcacgacc gcacctcggc gatcacgttc gaactgccgc
      181 gtgaattcga catggatgtt cgggcgatac gcgtcaagtc ggggaaagga gcgggaaaat
      241 gaagatcgtc tgccgttgtc aggatatcac cgaagaagaa gttctgagtg ccattcgcca
      301 gggggcgacc actctcgatg aaatcaaacg tctcgtgcgc gcaggaatgg gaccgtgtca
      361 gggtcgcacc tgccgacgac ttgtacaaca gattatctcg cgcgaactga agaagccgat
      421 ggccgagatt cacccgtcga cctttcgccc gccgaatctg ccggttccct tcaacctggt
      481 catggctgag tacgagaaga tcgagaagga gaaagagcgc aagaccgtgc gcaagccttc
      541 ggcaaagggg aaaggaaagg ccaaggaggg tgacgcatga agaccagtta tgatgcgatc
      601 gtgattggcg gtggtattct cggggtttcc atcgcgtatg aactggcccg tgccggcatg
      661 aagaacatct gcgttctgga aaagcactac cttgcgagcg gctcgaccgg acgttgcggc
      721 ggcggtttcc gccagcagtg gagcaccgag cacaatacac agctggccat ggattccgtg
      781 aagcggctcg agggtctgga gcaggaactc gagtacaact gtgagatgtt tcagggcgga
      841 tatctgattc tcgcgcacac cgacgcggat gtggcccagt tcgaaaagaa cgtggcgatg
      901 cagcggcgac tcgggcttga cgtgaatatg gtcgatccgg ccgaggcgaa aaagatcgtg
      961 ccccatctcg acacttccct gatcaagaaa gcgacctggt gtccgaaaga cgggcatatc
     1021 aacccgtttc tgctgaccca gggatacgcc aatgccgcca aacggctggg tgtcgacgtt
     1081 cacctgtgga ccgatgtgac cagtatcatg aagaaaagta acgtcttctt cgtcatgacc
     1141 aacaccgggt ttacctatca ggcccccatc gtcttcaact gtgccggcgg gtttgcccgc
     1201 gaaatcggca agatgctcaa cgtcgacatt ccggttgatc cgtatcgtca cgagattctg
     1261 gtgactgagc cggtcgaacg tctctggaac cccatggtca tcagcttcac cgtcggtctg
     1321 tatgcacgcc aggaagcgaa cggtggcgtg gtgatggggt gcggcgatcc gaatgagccg
     1381 gttggcacgt atgtcggcag ctcgatgcac ttcatgtatg atatgtcgaa gcgttttacc
     1441 acgctgtttc cgaaactggg gaatctgcgt atcgtgcgtc agtgggccgg attgtacgag
     1501 atgactgctg acgctcagcc gatcctgggc ccggttgatg aagtggaaaa cttctatcag
     1561 gccag
//
LOCUS       DAORHD010001303         1564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_107994_length_1564_cov_2.719682, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001303 DAORHD010000000
VERSION     DAORHD010001303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107994_length_1564_cov_2.719682"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="PKO06_21075"
     CDS             <1..1497
                     /locus_tag="PKO06_21075"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72214.1"
                     /translation="RKPAAQRELVPVLTWIRPKTSAEDCAMPIPGVPGEFTADQILGL
                     VKRLWDNRESARPEVRQQVEEFLPRVVPSLMKVAESDLIPESVRYDAVLLLGNVRTEE
                     ALPLLNRLVTDKLPAVRYASVTALAGYAPDEAVTPLLTALRDKHSLVRASAAQALGKL
                     RPSPALPALVKRMADTSRRVRLSAGDAIRQYPGEELATRLPFILPRASEAAMRRTLNL
                     CARLEDGLRHEVLRDLLKSRHAGILSHAVRVAMKAADPHLADELADAARYLEQLVPPA
                     PPVVAENTARKPKSATSSRQKPNADLPRETGASVSMQTSEAGRSPASTLPSFDEVLPP
                     VISHDESGSSTIAKTSIESVTPDCVRVVEPTPVSRSCFDMLSYQETFKCLTAFEQVRE
                     LRNLLEQQPLALEDLTKLYAGAAGAFVRSFVIDALKVHGPKAEPFLLLSLKEPEEETV
                     LAAIKTLADVGSDLALDSLAMLKRHASQHVRFTTEIAIEKIRSRKGKA"
BASE COUNT          324 a          445 c          466 g          329 t
ORIGIN      
        1 cgaaaacctg cggctcagcg cgagctggtt ccggttctga cgtggatccg gccgaagact
       61 tcggcggaag actgtgccat gccgattccg ggggtgcccg gcgagtttac tgccgatcag
      121 attctgggtc tggtcaaacg actgtgggat aaccgggaat ccgcccgacc ggaagtgcgg
      181 cagcaggtcg aagagtttct gccgcgggtg gtgccttcgc tgatgaaagt tgccgaaagc
      241 gacctgatac cggagtcggt tcgctacgat gcggtgttgc tgctcggcaa tgtccgaaca
      301 gaagaagcct tgccgctgct caaccggctg gtgacggata aacttcccgc cgttcggtat
      361 gcctcggtga ccgctctggc gggctatgcg cccgatgaag ccgtaacgcc gctgctgacg
      421 gctctgcgtg ataaacattc gctggtgcgc gcttcggcgg ctcaggcgct gggtaagctg
      481 cgaccatcac cggcacttcc ggcactggtc aagagaatgg ctgacacctc gcgccgggtg
      541 cgtctgagcg ccggagatgc gattcgccag tatccgggcg aggaattggc aaccagactg
      601 ccgttcattc ttcctcgtgc atcagaagcg gccatgcgtc gcactctgaa tctctgtgcc
      661 cgccttgaag atggtctgcg gcacgaggtg ctgcgcgatc tgctgaaatc acgccatgca
      721 ggaattctct cacatgccgt tcgcgtggcc atgaaagcgg ctgatccgca tctggccgat
      781 gaactggctg atgccgccag atatctggaa caactggtcc cgcccgctcc cccggtcgtt
      841 gccgaaaaca cagcgagaaa accgaaatct gccacttcat cgcggcagaa gccaaacgct
      901 gatctaccca gggaaacagg tgcttccgtg agtatgcaga cgtctgaggc cggcaggtcg
      961 cctgcttcga cgcttccttc cttcgatgag gtgcttccgc cagttatttc ccatgacgag
     1021 tccggttctt cgacgatcgc aaagacctcg attgagtctg tcactcccga ctgtgtgcga
     1081 gtggtcgaac cgacacctgt atccagatcc tgcttcgata tgctatcata tcaggagaca
     1141 tttaaatgtc tgacggcatt cgaacaggtt cgcgagttgc gaaatcttct tgagcagcag
     1201 ccgctcgcgc tcgaagacct gacgaagctg tatgccggtg cagccggggc gttcgtcaga
     1261 tcgtttgtca ttgatgcatt aaaagttcac ggtcccaagg ctgagccgtt cctcttgctg
     1321 tctctcaagg aaccagagga ggaaacggtg ttggcggcga tcaagacgct ggcagatgtc
     1381 ggcagcgatc tggctcttga ctctctggcc atgctcaaac gtcatgccag tcaacatgtt
     1441 cgattcacga cggagatcgc aattgaaaaa atccgctcca gaaaaggaaa agcgtaaacc
     1501 aagaccggag tatgaaccgg taccgagcgc ggtcgacccg agagccggtg atggtgatgg
     1561 tgcc
//
LOCUS       DAORHD010001304         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108196_length_1562_cov_4.520902, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001304 DAORHD010000000
VERSION     DAORHD010001304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108196_length_1562_cov_4.520902"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PKO06_21080"
     CDS             complement(<1..1530)
                     /locus_tag="PKO06_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72215.1"
                     /translation="MLPYRSGLDEKRSPKALYERAVQIDPCVLITLRSMEHIEFELTA
                     AAGGPKRRAFHETLTRGDRWFQSTLTLEPEGETNQYHVSDLSYDFAGVRESGKSSRTG
                     IMVGVRVDADGNPQPLPREASTIYSFLPTSEVSGLHFFVQGHFDLLVDRERIAKNSTW
                     NQRLFATVPEGLRLLAEHFLSAGTDDATTPELLAKKGMGFLDVLPLEDDLPDGFYRAT
                     LPQLSRLFRSYACVPGSEGSLIAPERLLIVDPGLAGFLGNRPVPWRSGEDRESGARYY
                     AQSPHLTDRQCLVAVSLGARFFGVSELLEMLHDSCTAQTKDTAAYSDLPFMQQPSAEQ
                     VLRLYGVLCAVIEREKDGPEGAAGKQLLEKIAGIPLLYDEKCVWRKFATDRYLKPARG
                     ARRYRELFDGLLTFVHPDTDLAFEGGRSGQPVWQRSAMLFDWLGIPVVDEEWVLTFLE
                     SATSPGNPQGRKLLDRLLEDPQRVKRLMDILVDASPAQQKRAGALPVFPRLGGGFNAV
                     AS"
BASE COUNT          327 a          510 c          434 g          291 t
ORIGIN      
        1 ggacgccacc gcgttgaatc cgccacccag gcgcgggaac accggaagcg ctccggcacg
       61 cttctgttgc gccgggctcg catccaccag aatgtccatc agtcgcttca cgcgctgcgg
      121 gtcttcgagc agccgatcca gcaacttcct tccctgtggg ttgccaggcg atgtcgctga
      181 ctcgagaaac gtcaggaccc actcttcgtc cactacggga atgccgagcc agtcgaacag
      241 catggccgat cgctgccaga ccggttgccc ggaacgccct ccctcaaacg ccagatcggt
      301 gtcgggatgc acgaaggtga gaagcccgtc gaacaactca cgataccgtc tcgcgccgcg
      361 ggcaggtttc aggtatcgat cagtcgcgaa tttacgccat acgcacttct catcgtacaa
      421 gagaggaatg ccggcaatct tttccaatag ctgttttccg gcagcacctt ccggtccgtc
      481 cttctctcgc tcaatcacgg cacacagaac gccgtacaga cgcaatacct gttccgcgct
      541 cggttgctgc ataaacggca ggtcgctgta ggcggccgta tccttcgtct gggcggtgca
      601 ggaatcgtgc agcatctcca gcaactcgct gacaccgaag aaacgcgctc cgagagaaac
      661 cgctaccagg cattgtcgat cggtcagatg gggtgactgg gcatagtatc gggctcctga
      721 ttcgcggtct tccccgcttc gccagggcac cggtcggttc cccagaaaac ccgcgagacc
      781 cggatccacg atcaacagac gttctggcgc aatcaacgat ccttccgaac cggggacaca
      841 cgcatacgag cgaaacaacc gcgataactg cggcagggtc gcgcgataga acccatccgg
      901 caggtcgtcc tcgaggggca gcacatcgag aaagcccatt cccttcttcg ccagcagctc
      961 tggcgtcgtc gcgtcatccg ttcccgcgct caggaagtgc tccgccagaa gacgaagccc
     1021 ctccggaacg gtcgcaaaga gacgctggtt ccaggtggaa ttcttcgcaa tccgctcccg
     1081 atcgaccagc agatcgaaat gcccctgcac gaagaagtgc agtccgctca cctccgaggt
     1141 tggcagaaaa ctatagatcg tcgacgcttc acgcggcaga ggctgagggt ttccatctgc
     1201 gtcgactcgg actccgacca tgatcccggt gcgactcgat tttccagact cgcgaacccc
     1261 cgcaaaatcg taggacaggt cagaaacgtg atattgattg gtctcgcctt cgggttcgag
     1321 cgtcaaggtt gactgaaacc agcggtcacc gcgggtcaga gtctcgtgaa aagcccgccg
     1381 cttggggccg cccgccgccg ccgtcaactc gaactcgatg tgttccatgc tgcgcagcgt
     1441 gatcaacacg cacgggtcga tctgaaccgc tctctcatag agagctttcg gagagcgctt
     1501 ttcgtcaagt cctgaccggt acggaagcac gacaaccgtc ccttcctccg ggatgtccgg
     1561 gg
//
LOCUS       DAORHD010001305         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108204_length_1562_cov_3.793630, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001305 DAORHD010000000
VERSION     DAORHD010001305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108204_length_1562_cov_3.793630"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKO06_21085"
     CDS             <1..735
                     /locus_tag="PKO06_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72216.1"
                     /translation="EAHRRLATRAMTKPVALDLNPFRRWLDSPFSRDQLDYLFFWREA
                     GFELEPVSQRCLFLQAVGEVIHCLSGFDGLNDLRPMPPDELMGYFLKQQQSRIFPRKG
                     SVRIFDSVERATGNLDAFCALVLPLVTAETPPKGYLADEYFQAWLNGDGDLERARAEL
                     LRLREPITIDWQGTMNVERLVPLAKGCCQTIVAWSGEHLPPHWYEEHLVKPLKAAFAA
                     VYTHFDWYVKGVDRQEEAYDFLLVMK"
     gene            785..>1562
                     /locus_tag="PKO06_21090"
     CDS             785..>1562
                     /locus_tag="PKO06_21090"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HNV72217.1"
                     /translation="MLRLLLLCFALAVPTPVSAGLPIYLMGEDAHGPDKLVLTVQGVQ
                     RKGFTGSLVSQKQQDVVEIAVSFFNQGRQAQTVDPATQLAYELNDRYTPVDAKTGTSV
                     LFRSFTVHPGTVSRGNLYFYVRSDDQRSQPRLIFESTSQRLEIVCNAELARLYEKRAG
                     AQLEVEEAERLGRFLIDAERMDEAAVLVRTAMERFPSSGLLRMQYAAILKWKNRPDEA
                     GEIIAQIVPDALGREDALAFARQAYDLGQFAMCRRVLEPFA"
BASE COUNT          305 a          435 c          477 g          345 t
ORIGIN      
        1 gaggcacacc gacggttggc gacgcgcgcc atgaccaaac cggtggcgct tgatctgaat
       61 ccttttcggc gctggttgga cagcccgttc agccgtgacc agctggatta tctgttcttt
      121 tggcgcgaag cgggttttga gctcgaaccg gtctcgcaac gctgtctctt tctgcaggcg
      181 gtgggtgaag taattcactg tctcagcggg ttcgatggtc tgaacgacct gcgcccgatg
      241 ccaccggacg aattgatggg ctactttctc aaacagcagc agagtcgtat cttccctcgc
      301 aagggcagcg ttcgcatctt cgactcggta gaacgggcca ctggaaacct tgacgcgttc
      361 tgcgcactgg tactgccgct ggtcactgcc gagactccgc ccaaaggcta cctcgccgat
      421 gagtatttcc aggcctggtt gaacggagac ggcgatctcg aaagggcaag agccgaactg
      481 ctgcggctgc gcgaaccgat caccatcgat tggcagggaa ccatgaatgt cgagcgtctg
      541 gtgccgctcg ccaaagggtg ttgccagacg atcgtggcct ggtcaggaga gcatctgccc
      601 ccgcactggt atgaagaaca tctggtcaaa ccgctgaagg cggcgtttgc cgcggtgtac
      661 acgcatttcg actggtatgt gaaaggcgtc gatcgtcagg aagaagctta cgattttctg
      721 cttgtcatga agtgaccccg ggcttttcgg cgagtatcgg ctgtgatacc atgcgggcat
      781 ggcgatgtta cgactgctgc tgctgtgttt tgctctggcc gttcccacac cggtctctgc
      841 cggtcttccc atctacctca tgggagaaga cgcccatggc ccggacaagc tggtactgac
      901 ggttcaaggg gttcagcgca agggtttcac cggtagtctg gtttcccaga aacaacaaga
      961 tgtagtcgag atcgcggttt cttttttcaa tcagggccga caggcgcaga ccgtcgatcc
     1021 ggcgacacag ctggcatacg agttgaatga ccggtacacg ccggtcgatg caaaaaccgg
     1081 aaccagtgtt ctctttcgct ctttcaccgt tcatccgggc acggtcagtc gcggcaactt
     1141 gtacttttac gtgcgttcag acgaccagcg ctcgcagccc agactcatct tcgagtcgac
     1201 gtcacagcgg ctcgagattg tctgtaatgc cgagttggca aggttgtatg agaaacgagc
     1261 cggcgcgcag ctcgaggtcg aggaagccga acgactggga cgctttctca tcgatgccga
     1321 acgcatggat gaagccgcgg tgcttgtgcg cacggccatg gagcgttttc cgagcagcgg
     1381 gcttctgcgc atgcagtatg cggccattct gaagtggaag aaccggccgg atgaagccgg
     1441 tgaaatcatc gcgcaaatcg ttcccgatgc gttgggtcgt gaagatgcgc ttgcgttcgc
     1501 gcgacaggcg tatgacctgg ggcagtttgc catgtgccgc cgcgtgctcg aaccgttcgc
     1561 cc
//
LOCUS       DAORHD010001306         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108421_length_1560_cov_5.439203, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001306 DAORHD010000000
VERSION     DAORHD010001306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108421_length_1560_cov_5.439203"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="PKO06_21095"
     CDS             <1..671
                     /locus_tag="PKO06_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNV72218.1"
                     /translation="QWGMRFSYAVQPKPEGLAQAFLIGRSFVAGEPSCLILGDNIFHG
                     AGLTATLTESASITKGARVFGYWVRDPERYGVVEFDEQGKAISLEEKPAVPKSHYAVP
                     GLYFYDGRVCDLAATLKPSKRGELEITDLNRRYLEAGELQVIRFQRGMAWLDTGTHES
                     LLQASNYIQTIEERQGLKVGCIEEIAFRQKFIDRSQLQRLAEPLAKNEYGRYLIDLAE
                     NHQP"
     gene            668..1219
                     /gene="rfbC"
                     /locus_tag="PKO06_21100"
     CDS             668..1219
                     /gene="rfbC"
                     /locus_tag="PKO06_21100"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNV72219.1"
                     /translation="MKFSPTVVPDVILIEPDVFNDPRGFFMETFHQGKFAAAGIRDHF
                     IQHNHARSAKGILRGLHFQHPKAQGKLIRVIAGEVFDVAVDIRPESQTFGQWVAETLS
                     ADNRRLLYIPPGFAHGYCVTSEFAEVVYCCTELYAPEFEGGLSWNDPDLKISWPVQNP
                     ILSGKDQKQPRLRDLFPLKFDRP"
     gene            complement(1298..>1560)
                     /locus_tag="PKO06_21105"
     CDS             complement(1298..>1560)
                     /locus_tag="PKO06_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72220.1"
                     /translation="SDSEDIRFIQPLGFDSAQNLILLYDTARERVLQRIRPDGTQEKN
                     ARFPLFDPGIELTTPWWLNPDGTISWVEMTPRQATIRRISIP"
BASE COUNT          350 a          450 c          434 g          326 t
ORIGIN      
        1 gccagtgggg catgcggttt tcctatgccg tgcaaccgaa acccgaaggt ctggcccagg
       61 catttctgat cggtcgcagc tttgtcgccg gcgaaccgag ctgtctcatt ctcggcgaca
      121 acatctttca cggcgccggt ctgaccgcga cgctgaccga gtctgcgagt atcaccaaag
      181 gagcgcgagt gttcggctac tgggtgcgtg acccggaacg gtacggggtc gtcgagttcg
      241 acgaacaggg caaagcgatc agtctcgaag aaaaaccggc agtaccaaag tcgcattacg
      301 ctgttcccgg gttgtacttt tacgatgggc gcgtctgcga tctcgccgcc acgctgaagc
      361 cctcgaaacg cggcgaactc gagatcaccg acctgaaccg ccggtatctc gaagcgggcg
      421 aactgcaggt aatccgcttt cagcgcggca tggcctggct cgacaccggt acacacgagt
      481 cgctgctgca agcgtcgaac tatattcaga cgatcgaaga gcgacagggg ttgaaagtcg
      541 gatgtatcga ggaaatcgcg ttcaggcaga agttcatcga tcgatcgcag ttgcagcgtc
      601 tcgcggaacc cctggccaag aacgagtatg gtcgctatct gatcgacctg gcagagaatc
      661 accagccatg aaattcagcc caaccgtcgt tcccgatgtc attctgatcg agcccgacgt
      721 cttcaacgat ccacgcggtt ttttcatgga aacctttcat cagggcaagt tcgcggccgc
      781 cggaattcgc gaccacttca ttcagcacaa tcatgcccgg tcggcaaaag gcattctgcg
      841 cggactgcac tttcagcacc cgaaagccca gggcaagctg atccgcgtca tcgcaggcga
      901 agtctttgac gtggccgtcg acattcgacc tgaatcacag acctttggtc agtgggtggc
      961 tgaaaccctg agcgctgaca atcggcggct cctgtatatt ccgcccgggt tcgcgcatgg
     1021 gtactgtgtg acttcagaat tcgccgaagt ggtctactgc tgcaccgagc tttatgcccc
     1081 cgaattcgag ggcggtcttt cctggaatga tcctgatctc aagatttcat ggccggtaca
     1141 gaatccgatt ctgtcaggga aagatcagaa gcaacccaga ctgcgcgacc tctttccact
     1201 caagttcgat cgtccgtaac cgcccagacc aaaagtacaa gtcagagaac cgaaccatca
     1261 gcttcgaaag agcagatggt tcggttggtc tttacctcta cggaatggaa atgcggcgaa
     1321 tggtcgcctg acgcggggtc atttcgaccc aggagatggt gccatccggg ttcagccacc
     1381 agggcgtggt taactcgatc cccggatcaa acagcgggaa tcgagcattt ttctcctgcg
     1441 tgccatccgg tcgaatgcgc tgcaacacac gctctcgcgc cgtgtcatac agcagaatca
     1501 gattctgcgc cgagtcgaag cccagcggtt gtataaatcg aatgtcttca gaatcgctga
//
LOCUS       DAORHD010001307         1560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108445_length_1560_cov_3.373422, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001307 DAORHD010000000
VERSION     DAORHD010001307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108445_length_1560_cov_3.373422"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="PKO06_21110"
     CDS             complement(<1..1112)
                     /locus_tag="PKO06_21110"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72221.1"
                     /translation="MRVSRSVMLMFTLFSLVSSALLAGERSASEVLLEGKQLEKAGKT
                     AEAVEVFRKAIREHPSEALYSACGSLLGKLSKLNDAATLLNEGLKQFPDSPSLLNVRG
                     LVLLRQGKPEQAAQDWKKVLAREPNNAFARDWLKKAEKPGAAAARKPATPGVEPGSTP
                     EPEPTPDVPAKASGLNDQTLTIKPLAQPGSPEEQAKKAKAIYTQMAGMSNNDLDALEA
                     LHLQVIQECPDTDFARESIFMLSNMYQFATANPLIEKQSALLDYLLEKYPGSDPAVLL
                     MNRIVNTYRAAGKPEKLVGVYSKALTNPNIDDRSFIIYGLDYAKALQETGKKDEARNM
                     LTAVIARDGTNNSFEAGVAKNLLTQWKPGWDALRAL"
     gene            complement(1118..>1560)
                     /locus_tag="PKO06_21115"
     CDS             complement(1118..>1560)
                     /locus_tag="PKO06_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72222.1"
                     /translation="FTKTTDMINAQVLLESIVERLRSDARCMITKIEQNESGIRFKTR
                     RAGRETTIVYRHDPTRKAIVREEFGPDGTQVSQRDFQSQGRISLLHFQFGRMERPGVR
                     DAVDHIDLVLHISISERPDTPATNLSIICQVFSTCVQLPNPYRR"
BASE COUNT          286 a          406 c          492 g          376 t
ORIGIN      
        1 ttgagggcgc gcagcgcgtc ccagcccggc ttccactggg tgagcaggtt cttggcgact
       61 ccggcttcga agctgttgtt cgtgccgtcg cgagcaatga ccgcggtgag catgttgcgg
      121 gcttcgtctt tcttgccggt ttcctgcagg gctttggcat agtcgagacc gtagatgatg
      181 aacgagcggt catcgatgtt cggattcgtc agggctttgc tgtagacccc gacgagcttc
      241 tcgggtttgc cggcggcgcg gtaggtgttg acgatgcggt tcatcagcaa cacggccgga
      301 tcagaaccag gatacttttc gagcagataa tcgagtagcg ctgactgctt ttcgatcaat
      361 ggattcgccg tggcaaactg gtacatattc gacaacatga aaatggattc ccgggcaaag
      421 tccgtgtcgg ggcattcctg aatgacctgc agatgcaagg cttcgagggc atcaaggtcg
      481 ttgttggaca ttcccgccat ctgggtgtag atcgctttgg ctttcttcgc ttgttcttcg
      541 gggctgcccg gctgggcgag cggcttgatc gttagcgtct gatcgttcaa ccctgaggcc
      601 ttggccggaa cgtcgggtgt cggctccggt tccggggtgc tgccgggttc gacgcctggc
      661 gtggcgggtt tcctggctgc tgccgcacct ggtttctctg ctttcttcag ccaatcacgc
      721 gcaaatgcgt tgttgggttc tctggcaagt actttcttcc aatcctgggc tgcctgttcc
      781 ggcttgccct gtcgcagcag gactaacccg cgaacattga gcagtgaggg actgtcagga
      841 aactgtttca acccctcgtt gagcagggtg gcggcgtcgt tgagtttcga cagtttgcca
      901 agcagagatc cgcatgcgga atacagggcc tcggaggggt gttcgcgaat ggccttgcga
      961 aagacttcaa cggcttcagc ggtcttgccg gctttttcca gttgcttgcc ttcgagcagc
     1021 acttcgctgg ctgaacgctc gccggccagg agagctgacg aaacgagact gaagagggtg
     1081 aacatgagca tcacggagcg ggacacgcgc atgaggtcta cctcctgtag gggttcggaa
     1141 gctgaacgca ggtcgagaac acctggcaga tgatcgagag attcgtggcg ggggtatcag
     1201 gacgttcact gatggagata tgcaggacca ggtcgatgtg gtcgacggcg tctcggacgc
     1261 ccggacgctc cattctacca aactggaagt gaagaaggga aatgcgaccc tgactctgga
     1321 agtcacgttg cgagacctgt gtgccatccg ggccgaactc ctcgcgaacg atggctttcc
     1381 tggttggatc atgacgatag acgatcgtgg tttcgcgacc cgcacgcctg gttttgaagc
     1441 gaatgccgct ttcgttctgt tcgatcttcg tgatcatgca acgtgcgtcc gagcgaagac
     1501 gttcaacaat gctttcgaga agcacctgag cgttgatcat atccgttgtt ttcgtgaacg
//
LOCUS       DAORHD010001308         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108693_length_1558_cov_3.269461, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001308 DAORHD010000000
VERSION     DAORHD010001308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108693_length_1558_cov_3.269461"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PKO06_21120"
     CDS             complement(<1..852)
                     /locus_tag="PKO06_21120"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HNV72223.1"
                     /translation="MRIYSITPDDEFKEYAEIEFKDEHQESILESWLENNPEKIIENG
                     ALLIIGRQVATNLGGFIDLLGIDRQGDLVVIELKRDRTPRETLAQSLEYASFAEKLGY
                     EQLDEIFRDYSGDEALNLAQYHREYFNLISDEAVVFNNDQRIVILGRRVTPEIRQTSA
                     FLRKKGVRVTCLEFTFFRADGGLRLFSTNIVVGNEPEGITRVSSGSLPVISEREFLAS
                     LDDNGRIVFGKLLAWAKTNSFPIHWGTKGFSLNVDVSGTHVAFCFGYQPKSVFRQSIY
                     SALVGRGG"
     gene            complement(1023..>1558)
                     /locus_tag="PKO06_21125"
     CDS             complement(1023..>1558)
                     /locus_tag="PKO06_21125"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV72224.1"
                     /translation="RLLHCRAGFRSVAGRAEATTLAAGSVDFIVAGQAFHWFDQDRAR
                     AEFLRILRPSGWVMLVWNERDTRRTPFLMYYERVLRRHAVDYEQVGRRRVQARRLAGF
                     YGESGFWTREFHHRQTLDHDGLVGRVLSASYAPEPGHPAYLPMLAELERLFQNHQVDG
                     AVAFDYTTTMYCGRLDS"
BASE COUNT          382 a          478 c          374 g          324 t
ORIGIN      
        1 accgccgcgc ccgacgagtg ctgaataaat agactgccta aagacggatt ttggctgata
       61 gccaaagcag aaagccacat gagttccaga tacatcaacg ttaagagaga agccttttgt
      121 gccccaatgt atcggaaacg aatttgtttt tgcccacgcc aacaacttcc cgaaaacaat
      181 ccgtccattg tcgtccagtg aggcaagaaa ctctctttca gagatgacag gcaatgaacc
      241 tgacgatacc ctggtaatgc cttcgggttc attaccgaca acgatgttcg ttgaaaacag
      301 gcgcaaacca ccatcggccc tgaagaaggt gaactccaaa caagtgactc ttacgccctt
      361 ctttctcaag aatgccgatg tctgacgaat ctccggcgtg accctccggc ctagaatgac
      421 gattctttgg tcattattga atacaaccgc ttcatcggaa atgagattga agtactctcg
      481 atggtactga gccagattca gagcctcatc tcccgagtaa tcacgaaaaa tctcgtccaa
      541 ttgctcataa ccgagtttct ccgcgaagga agcgtattca agagactgcg cgagagtttc
      601 gcggggagtg cggtctctct tcagctcgat gacaaccaga tcgccctgtc tatcaatccc
      661 cagtagatcg atgaagccac cgagattcgt ggcgacctgc cgaccgatga tcagcaaagc
      721 cccgttttca atgatttttt caggattatt ctcgagccag ctttccagga tcgactcctg
      781 gtgctcgtcc ttgaattcga tttcagcata ttccttgaat tcgtcatccg gcgtgatact
      841 gtaaattctc attgtgtttt cctcatgcta gtttcacgat ttccatgctg acaacgccca
      901 tcttgctcga aggcgaacac tcccgccctt ggagaccacc ctgaaacccc aacagcccgg
      961 actcaacgcc tccagcccgt gcgagcgaag cgtttcggct gcatgccgcg gccgggccgg
     1021 cattacgagt cgagcctgcc gcaatacatg gtcgtcgtat agtcgaaagc caccgcccca
     1081 tcgacctgat gattctgaaa aagccgctcc agttccgcca gcatgggcag ataagcggga
     1141 tgccccggct ccggcgcgta tgaagccgac aacacgcgcc ccaccagacc gtcatgatcg
     1201 agtgtctgcc ggtgatgaaa ctccctggtc caaaaaccgc tctcaccata aaagccggcg
     1261 agccgccggg cctgaacgcg ccgacggccg acctgctcgt aatcgacggc atggcgccgg
     1321 agaacacgct cgtaatacat gagaaaaggc gtccgtcgag tgtcccgttc attccagaca
     1381 agcatcaccc agccagacgg tcgcagaatc cgcagaaact cggcccgggc ccgatcctga
     1441 tcgaaccagt ggaacgcctg tcccgcgact atgaaatcca cactccccgc agccagagtc
     1501 gtcgcctcgg cccggccggc cacgctcctg aaacccgccc ggcagtgcag aaggcgtt
//
LOCUS       DAORHD010001309         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108804_length_1557_cov_3.296272, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001309 DAORHD010000000
VERSION     DAORHD010001309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108804_length_1557_cov_3.296272"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1132
                     /locus_tag="PKO06_21130"
     CDS             311..1132
                     /locus_tag="PKO06_21130"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNV72225.1"
                     /translation="MGSLEFSHHTDVGKKRTHNEDSLVAIPPWRDPAFSCQSCFFGVA
                     DGMGGHAAGEVASRLAIEGILYWMAHNQITSLSQSHIENAFGEASSRIWSYAQEHQEK
                     KGMGTTLTGILIHGSQALLGHIGDSRCYLLREGKLRQISRDHTLVAEQIRQGKLTPED
                     AKKHPMRHILSRAMGVREFAEVDTMLLDLAPGDLFLFCTDGVYNWMPDERLEQILGKA
                     EFPQVTRQLVEEAVQVGGDDNATAVMVRIRELPVDIPGRFSLGRVKGLWYDWCNF"
     gene            1220..>1557
                     /locus_tag="PKO06_21135"
     CDS             1220..>1557
                     /locus_tag="PKO06_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72226.1"
                     /translation="MDMFTIVIIVVVLALLAIVAVLLMSNKSGETQPAAAEAPAGEEK
                     KENPTAKRFKQVLSGNDLQSEMKKEEQELLTKYTAGTGEGIAEVEKLLVSDPNNVDLL
                     DWLAFMYYSN"
BASE COUNT          366 a          419 c          454 g          318 t
ORIGIN      
        1 cctaggacat agatcggcat gaccgagaac gacatgctga acaggcgaat agccagaaag
       61 gcaaacgcca gcaggatagg aatcgcggcc cagcgcaggg cgacgagact ctgtgcaatc
      121 tgcagttcac gcaagccgga gcgtccgttg tgttccatag tgcttctcca gaaacggcga
      181 caataatcca cttcctgata tcggcactga caggcaattc cttgaccggg gttttgaacc
      241 ccggtataat tcgaacatca gcggcgggca atttctcgtt tcgcgccgga agtacatcac
      301 gtgggggatt atgggctcac tggaattctc gcatcacacc gatgtcggga aaaagcgcac
      361 gcacaacgag gattccctgg tggccattcc accctggcga gaccccgcct tctcgtgtca
      421 atcctgtttc ttcggcgtcg ccgatgggat gggaggacac gccgccggcg aagttgcttc
      481 tcgtctggcc atcgaaggca tcctctactg gatggcgcac aaccagatca cctcactgag
      541 ccaatcccat atcgaaaatg cctttggtga agcgagtagc cgcatctgga gctacgcgca
      601 ggaacaccag gaaaagaagg ggatgggcac aacgctcacc ggtatcctga ttcacggttc
      661 gcaggctctc ctcgggcaca tcggcgattc gcgctgttac ctgttgcgag agggcaagtt
      721 gcgtcaaatc tcccgtgatc acaccttggt tgccgagcag atccgtcagg gaaaactgac
      781 accggaagac gccaagaaac atcccatgcg tcacatcctc agccgcgcca tgggtgtgcg
      841 tgagttcgcc gaggttgata ccatgctgct cgacctcgcc ccgggagatc tgtttctctt
      901 ctgcacggac ggcgtctaca actggatgcc ggacgaacgg ctcgaacaga ttctgggcaa
      961 agccgagttt ccgcaggtga ctcgtcagct ggtcgaagaa gcggttcagg tcggtggtga
     1021 cgacaatgcc accgccgtga tggttcgcat tcgagaactc ccggtggata ttcccggcag
     1081 attctctctt ggaagagtga aaggcttgtg gtacgattgg tgcaatttct gattctgatg
     1141 gacgcacgtt gttttccgca atcgacacct ggtggatcgt ggaagcacgt ggtttgaagc
     1201 caacgaggag gttcggggta tggatatgtt tacgatcgtc atcatcgttg tcgtgctggc
     1261 gctgctcgct atcgtggcag tgttgctgat gtcaaacaag tcgggggaaa cccagccggc
     1321 tgccgcagaa gccccggcag gcgaagaaaa gaaagaaaat ccgaccgcga agcggttcaa
     1381 acaggtgctc tcgggcaacg acctgcagag tgagatgaag aaagaagaac aggagctgct
     1441 gacgaagtat acggccggca ccggcgaggg cattgccgaa gtcgaaaaac ttctggtttc
     1501 tgacccgaat aacgtcgatc tgctcgactg gctggcattc atgtattaca gcaacaa
//
LOCUS       DAORHD010001310         1557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_108817_length_1557_cov_3.025300, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001310 DAORHD010000000
VERSION     DAORHD010001310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108817_length_1557_cov_3.025300"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKO06_21140"
     CDS             complement(<1..1144)
                     /locus_tag="PKO06_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72227.1"
                     /translation="MNRQVWARLLALLVLVVVQLVPAFGLDGASAISSRSVACGYVAW
                     GTLYDRLIEYLQGLGDNPSAEKLQVVELARTALQPLAAARPGDAPTKACLSNLRVLLG
                     AVEMYNMDHKEMQHSLDVGVLVQKTYLKLAPACPSGGDYSSKGDLAESGVIVCSKHGT
                     VDEPIQDEQDKRPTPFPETAQALAAEFLEFHARAEFRPSGAIWISLDNDLRPTIVCQA
                     TIKPRELLESVARRFIGLPKPKSVDENRVQFEFYPSQWGKQPLVIEILRDGITVRVDP
                     AARDQAGVGSGDSWKEFLTQAAGPEVISFFELDGERLREEMQRTLSTERRDWCLANLD
                     LLAAAVTSYNQTVKEPMDRLDLQKLIGSRILRCEPRCPDQGAYSSAG"
     gene            complement(1165..1419)
                     /locus_tag="PKO06_21145"
     CDS             complement(1165..1419)
                     /locus_tag="PKO06_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014610083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72228.1"
                     /translation="MSEFVLRHIKIIELIGVLMRIFSFSLVSWLGPASPFLFVWVFNT
                     IDAVMLSYCSVLKKDLAYSILNVFWILVGVVGITRAMGII"
BASE COUNT          340 a          509 c          426 g          282 t
ORIGIN      
        1 cgcccgccga cgagtacgcg ccctgatcag ggcaacgcgg ctcacagcgc agaatcctgc
       61 tgccgatgag cttctgtaga tccaggcggt ccatcggctc tttcaccgtc tggttatacg
      121 aggtcacggc ggcggcaaga agatcgaggt tggccagaca ccagtcgcgc ctctccgtgg
      181 aaagggtccg ctgcatttcc tctctgagcc tctcgccatc gagctcaaag aagctgatca
      241 cttcggggcc ggctgcctga gtgagaaact ccttccagga atcgcccgaa ccgacaccag
      301 cctgatctcg agctgccggg tcgacacgca ccgtgatgcc gtcgcgcaga atctcgatca
      361 ccagcggttg ttttccccac tgcgacgggt aaaactcgaa ctgcacccga ttctcatcga
      421 cactcttggg cttcggcaga ccgatgaaac gtcgtgccac ggactccagc agttcacgtg
      481 gtttgatcgt cgcctgacag acgatggtcg ggcgcaggtc gttatcgagc gagatccaga
      541 tcgcacccga aggacgaaac tccgcccgcg catgaaactc cagaaactcg gcggcaagcg
      601 cctgcgccgt ctcgggaaag ggtgtcggcc gcttgtcctg ctcgtcctga atcggctcat
      661 cgacggtgcc atgtttcgaa cagacgatca ctccactttc cgcgagatcg cctttcgaag
      721 aatagtctcc ccccgacggg caagccggcg ccagcttcag atacgtcttc tggaccagca
      781 cgccgacatc gagcgaatgc tgcatctcct tgtggtccat gttgtacatc tcgaccgcgc
      841 cgagcagcac ccgcaggttc gagaggcagg ctttcgtcgg ggcgtcgccc ggtcgggcag
      901 ccgccagtgg ttgcagggca gtgcgtgcca gctcgaccac ctgcagcttc tcagccgacg
      961 gattgtctcc gagcccctgc agatactcga tcagtcgatc gtacaatgtg ccccaggcca
     1021 catacccgca tgcaacggac ctcgagctga tagcggaagc accatcgagc ccgaacgccg
     1081 gaaccagttg aacgaccacc aggaccagaa gcgcgagaag ccgcgcccac acctgccgat
     1141 tcatgcgatc ctcccagata ttgtctagat gatccccatc gcccgggtga tcccgaccac
     1201 gcccaccaga atccagaata cattgagaat agagtaggcc agatctttct tcaataccga
     1261 gcagtatgaa agcatcacgg catcgatggt gttgaagacc cagacgaaga gaaacggact
     1321 tgccggcccc agccagctga ccagggaaaa gctgaaaatg cgcatcagga caccgatcaa
     1381 ctcgatgatc ttgatgtgcc tgagtacaaa ttcactcatc ggccactcct ctctgatagc
     1441 ggcttcgggg ccgtcgaagt tcaaccctgc aaaccaacgc cggcatgcgg gaacctcgtc
     1501 agtcgccgaa agtcttccag accgttcacc tcgggccgac cgggaacgcg cgtcagc
//
LOCUS       DAORHD010001311         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_109049_length_1555_cov_3.094000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001311 DAORHD010000000
VERSION     DAORHD010001311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109049_length_1555_cov_3.094000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1363
                     /locus_tag="PKO06_21150"
     CDS             212..1363
                     /locus_tag="PKO06_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72229.1"
                     /translation="MSKNKRSNERLCGHLSTRLGFAIPTAILAVFVAAFFLATLSTMN
                     RGYKAGLQHLDARQHMFNVAFSAYSSVLARLSEKAWNDRFFKGAPFAQSGVNHDDATY
                     DLYVTDTAGKQYQADLYIRVKVGKASQLFFWRFQYVDDILDTSGRAYPLVFLDAEADY
                     YPGGSKSLDAKIADILQQRKQNRPKAMGKTKLAQTLTSVNDVAQVVTARPQGTLIPES
                     AVGTQNPSAPAIPGFYDDSNDRGQLGDKTPPPAPSNPPEYTGPQEFGDSGSGVRVATK
                     GGTVSVNVCCINEKGTRVMVAVNGNPVLSWKSKEDHSYLVNKGNWIKKVAGMDPVEKE
                     RSKQEFNKFVRKSFKKTFTISPGDKVTTSYVGKKSPTGHKAWFQLTGPF"
     gene            1369..>1555
                     /locus_tag="PKO06_21155"
     CDS             1369..>1555
                     /locus_tag="PKO06_21155"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72230.1"
                     /translation="MNQRSREYKMSRAGWAMKRGKRAFTLVELLVAGAIFILIMTLAY
                     RMNVASTQKTLTGKLVLQ"
BASE COUNT          390 a          420 c          429 g          316 t
ORIGIN      
        1 ggggtacctc gatggccttg agccaacatc gggtgaggtc tgctacttct cgctcgtctc
       61 gattgatggt cgacactcaa ccaatcttat cgaagggcgt tggccgtgat cttcgggtat
      121 cgagctcgtt cagttccact ttcttctctt cgctgctaca atgaagaagg aagaggagag
      181 ccttacgtgc agggaaccaa gaggtatgag catgagtaag aacaaacgca gcaacgagcg
      241 gttgtgtggc cacttgtcaa ctcgtcttgg gttcgccatt ccaacagcga ttctcgcggt
      301 gtttgttgcc gcgttttttc tggccacgtt gagcaccatg aatcgcggat acaaggccgg
      361 gcttcagcat ctcgacgcac ggcagcatat gtttaacgtg gcgttttcgg cctattcgag
      421 cgttctggct cgactgagcg aaaaagcctg gaacgatcga ttctttaagg gcgctccttt
      481 tgcccagagc ggcgtcaatc atgacgacgc aacctatgat ctgtacgtca ccgacaccgc
      541 gggaaaacag tatcaggctg acttgtatat ccgcgtgaaa gtcggaaaag ccagtcagct
      601 ctttttctgg cggtttcaat acgttgacga cattctggat acctcgggac gcgcgtatcc
      661 actggtcttt ctcgatgccg aagccgacta ctatcccggt ggcagcaaga gcctggacgc
      721 caagatcgcg gacattctgc agcagcgcaa acagaaccgg cccaaggcca tgggcaagac
      781 caaactcgct cagaccctga cctccgtgaa tgatgtcgcg caggtcgtga ccgcgcgccc
      841 ccaggggacc ctgattcccg agtcagccgt cggaactcag aatccctcgg ctcctgccat
      901 tcccggcttt tatgacgaca gcaatgatcg cggacaactg ggagacaaaa ccccgccacc
      961 ggcacccagc aacccaccgg agtacaccgg accacaggag ttcggggaca gcggcagtgg
     1021 cgtacgggtt gccacgaaag gtggcacggt ttcggtgaat gtctgctgca tcaacgagaa
     1081 gggcacgaga gtcatggtag ccgtcaacgg gaacccggtt ctgagctgga aaagcaaaga
     1141 agatcacagt tatctggtga acaagggaaa ctggatcaag aaggtcgccg gcatggatcc
     1201 ggtcgagaaa gagagatcca aacaagagtt caacaaattc gtgcgcaaga gtttcaagaa
     1261 aacctttacc atcagccctg gtgacaaagt cacgaccagt tacgtcggga agaaatcacc
     1321 gaccggccac aaagcatggt ttcaactgac cgggccgttc tagaaaccat gaatcaaaga
     1381 agccgagagt acaagatgtc gcgagccggg tgggccatga aaagagggaa aagagccttt
     1441 actctcgtag agcttctggt cgccggggca atcttcattc tcatcatgac tctggcttac
     1501 cgcatgaatg ttgcctcaac gcagaaaacc ctgacgggca aactggtact gcaga
//
LOCUS       DAORHD010001312         1555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_109051_length_1555_cov_3.030000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001312 DAORHD010000000
VERSION     DAORHD010001312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109051_length_1555_cov_3.030000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKO06_21160"
     CDS             <1..845
                     /locus_tag="PKO06_21160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72231.1"
                     /translation="AKVVRDYDTALSVVADDSRLGQVFLNLIVNAAQAIPEGDVDHNE
                     IRISARLLDGNIVVEVSDTGQGISTSLLEKLFQPFFTTKEQGKGTGLGLATVFGIVSQ
                     NQGQIKVSSTPGRGTTFSLYFPRSTASLPAATPTPTAETRPVSTATRSLTILLVEDEP
                     SLLEISQLMLERCGHTVLTAHSPLTALKIEQEHSGALDLLMTDIMLPEMNGRELRDRL
                     IARRPSLQTLFISGYTADVIARQGLIESGVHFLQKPFNRQKLLDKLAEILPLIPEAKL
                     TPTS"
     gene            992..>1555
                     /locus_tag="PKO06_21165"
     CDS             992..>1555
                     /locus_tag="PKO06_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72232.1"
                     /translation="MHTRQDQPVLPPELRQGVSSVALVSLAMVLLFSSLTIIGGIWFV
                     REMSGSIRQQKLLELQAIAELKNMQVADWRRQMISGAQLFASGWARRLVDQLQETHDA
                     AALREPLIQRLTAFREVFGYQDFFLTTVTGNVLVTTSSHTHELPDISRQHVQQAVTSG
                     GPFMSEPFVCPHCSLTHIEVTVPLLSSS"
BASE COUNT          310 a          502 c          423 g          320 t
ORIGIN      
        1 gagcgaaagt ggtccgtgac tacgacaccg cgctgtcggt cgtcgccgac gactcacgcc
       61 tcgggcaggt ctttctgaac ctcatcgtca acgcggccca ggccattccg gaaggcgacg
      121 tggaccacaa cgagatccgc atttctgcgc ggctcctcga tggaaatatc gtcgtcgagg
      181 tctccgatac gggccaaggt atttctacca gcctcctcga gaaactcttt cagccgttct
      241 tcaccaccaa agagcagggg aagggcaccg gtctgggcct ggcgaccgtc ttcgggattg
      301 tcagccagaa ccaagggcag atcaaagtct ccagcactcc cggccgtggc acgacctttt
      361 ccctgtattt tccgcgcagc acggcttccc tgccggcagc aaccccgaca cccacagcag
      421 aaacgcgccc tgtcagtacc gccacccgtt cactgaccat tctgctcgtc gaagacgagc
      481 caagcctgct ggagatctca caactcatgc tcgagcgctg cggacacacg gtgctcaccg
      541 ctcactctcc tctcacggca ctgaagatcg agcaggagca cagtggagcg ctcgatctgt
      601 tgatgaccga catcatgctc cccgagatga acgggcgcga actgcgcgat cgcctgatcg
      661 cccggcggcc aagcctgcag acgctgttca tctccggcta cacagccgat gtcatcgccc
      721 gacaggggct gatcgaatcc ggggtgcact ttctgcagaa accgttcaat cgtcagaagc
      781 tgctcgacaa gctggctgag attcttcccc tgattcctga agcgaaactg acgccgacct
      841 cctgagttct ccgcgggggg tgtggctgca tgtctgaggt tccttcagaa accggcacgc
      901 gtatcacgct gtaacgcttg atttgtgcta tgctgcagag agaactgctg aagtgtcgga
      961 actctttccg aactttccac atgtgagccg tatgcacacc agacaggatc aacccgttct
     1021 accgcctgaa ctccgccagg gcgtctcctc agtagccctc gtgtccctgg cgatggttct
     1081 cttgttcagc agcctgacga tcatcggggg tatctggttt gtccgagaga tgtcgggctc
     1141 gattcggcaa cagaagctgc tcgagctgca ggcgattgca gaactcaaaa acatgcaggt
     1201 tgccgactgg cgtcgacaga tgatctccgg tgctcaacta ttcgcttccg gctgggctcg
     1261 ccggctggtc gaccagctgc aggaaaccca cgacgccgcg gccctacggg agccgctgat
     1321 tcagagactg accgcttttc gcgaggtctt cggctatcag gatttctttc tgaccacggt
     1381 caccggcaac gttctcgtga ccacaagttc gcatacccat gagcttccag acatcagtcg
     1441 gcagcatgtg cagcaagccg tcacgtcggg cggcccgttc atgagcgaac cgttcgtctg
     1501 cccccactgt tcgctcacgc acattgaagt cacggttccc ctgctgtcga gcagt
//
LOCUS       DAORHD010001313         1553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_109248_length_1553_cov_4.037383, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001313 DAORHD010000000
VERSION     DAORHD010001313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109248_length_1553_cov_4.037383"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..948)
                     /locus_tag="PKO06_21170"
     CDS             complement(172..948)
                     /locus_tag="PKO06_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin hydroxylase family protein"
                     /protein_id="HNV72233.1"
                     /translation="MDDHSHFLAERRQRVAEYPKNSSLQHASESFLVESIKNHYSYNF
                     DWMGLPIIQYPQDLIALQEIIWETRPDCIIETGVARGGSLVFYASMLELLGGDGFVVG
                     IDIDIRAHNRRRLMEHPLMRRLRLIDGSSVSTDIVQVVESLLSDRKRVMVCLDSNHTH
                     DHVFQELQKYAPLVTPGCYCVVFDTIVEDMPAGFFTDRPWDKGNNPRTAVSAYLQHLQ
                     TTSPIDRHGCAVQFVVDHAIDARLLVSAARGGWLRRRPRD"
     gene            complement(1339..1476)
                     /locus_tag="PKO06_21175"
                     /pseudo
     CDS             complement(1339..1476)
                     /locus_tag="PKO06_21175"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          376 a          394 c          465 g          318 t
ORIGIN      
        1 tgtgccgcac tggatgagcg tcttttcccc tgatatggtg aggtcatcga acaaagaata
       61 cgtaaaacga cccaggacag aaccgatccg ctgctggcag atgggacagg cgcgagttgt
      121 ttcgactctg tccatcatga cgtgcgtgtg ttcttcatgg gggatgtgcc ttcagtcgcg
      181 cggtcggcgt cgcagccagc caccacgcgc tgcgcttacc aacaaccgcg cgtcgatggc
      241 gtgatcgacg acaaactgca cggcgcagcc gtgacgatcg atgggcgagg ttgtctgcag
      301 atgctgcaga tacgcagaga cagccgttcg cggattgttg cctttgtccc agggccgatc
      361 ggtgaaaaag ccggcgggca tgtcttcaac gatcgtgtcg aaaacgacgc agtaacagcc
      421 cggtgtgacg agaggtgcgt atttctggag ctcctgaaaa acatgatcgt gtgtgtgatt
      481 ggaatcgagg cagaccataa ccctctttcg atcagagagt aagctctcga caacctggac
      541 aatatcggtg ctgacgctcg atccgtcgat caaacggaga cgccgcatca gcggatgttc
      601 catgagtcgg cgacgattgt gagcgcgaat gtcgatatcg ataccgacaa caaagccgtc
      661 acctcccagc aactcgagca tcgacgcgta gaatacgagt gaaccgcccc gagcgacgcc
      721 tgtttcgata atgcaatcgg ggcgtgtttc ccagatgatt tcctgcaggg caatgagatc
      781 ctggggatac tggatgatcg ggagtcccat ccaatcaaaa ttgtacgaat agtgattctt
      841 gatgctttcg acgagaaagg actcggaggc atgttgcagc gacgagttct tgggatactc
      901 ggctacacgt tggcgccgtt cggcgaggaa atgggaatgg tcgtccatga tagtctcctg
      961 ggatggcgtt ggcatgccga ctccacaaag ccggcacagc cgttcgagat ccgcatcagg
     1021 cgggagaagg cgaaagtagt gtgccggtgc accccgggtg gcataccgaa aagacgccgg
     1081 aatggaaaac ccctcctgac cgcgtgtcag cagattcaaa gcgatatctt gctcggaatg
     1141 accggtacag gaagaaagaa aaagatggaa cggagttccc gctcgaacgt gctcgatcgg
     1201 ttcaagaatc agcgtgcgag ctttctttgt acaagacagc aattcagaaa agagtttatg
     1261 aaagagcttg acgctgatat caatatggca ctcgttcatg acgaaacgat gtcctgtatc
     1321 ggcgagtgcc tgacgatagg ctggatccgt cgccagtcga tggagggctt ccgaaaactc
     1381 gccaggggtg ttggcgacaa tgccagtttc accatgtcgg atcagcgcct gctccggcgg
     1441 gttggcaaaa acgacgggga cggctccata ggccatggcc tcaatgagta cctgttctcc
     1501 ggtgccataa tgctgcggct caagcggata agcaaacgcg tgaagtcgta gga
//
LOCUS       DAORHD010001314         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_109935_length_1547_cov_3.394102, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001314 DAORHD010000000
VERSION     DAORHD010001314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109935_length_1547_cov_3.394102"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="PKO06_21180"
     CDS             complement(<1..620)
                     /locus_tag="PKO06_21180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV72234.1"
                     /translation="MTLRLHHLTFTHANSSTPLFEDLSLHVSAGWTGLIGPNGSGKTS
                     LLELAAGVLKADSGSVEREGFPVFCAQRTDAPPDDQRSFAEAVDAEASRLRRLLHIES
                     DWHERWESLSHGERKRLQIAVALWKNPDILLIDEPTNHLDRDSLDFLHQALHGFTGTG
                     ILVTHDRHLLDSLCQVCIFLEPGGIRRYPGSYTQAREQKQRDEVSV"
     gene            921..1304
                     /locus_tag="PKO06_21185"
     CDS             921..1304
                     /locus_tag="PKO06_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72235.1"
                     /translation="MKGIVTGCQKTQSGCLCRLQGPCGNITGLWRGDRPPEIGTECRV
                     EITLALNGSSPRESDVTAQTECDDRSGMNQLSGLVEQVSEDGVGLLRMAADAVVMLPG
                     VPARLDKTRVTIQVPFDQTELYPES"
     gene            complement(1305..>1547)
                     /locus_tag="PKO06_21190"
     CDS             complement(1305..>1547)
                     /locus_tag="PKO06_21190"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012864223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNV72236.1"
                     /translation="REIPYGETRTYAQIAHIAAQRLGRRSMSARAVGQAIGRNPISIL
                     IPCHRVIGSDGQITGYAGGVDRKTALLKLEREHRRA"
BASE COUNT          319 a          418 c          480 g          330 t
ORIGIN      
        1 tttacactca cttcgtcgcg ctgtttctgt tcacgtgcct gagtatagga cccgggataa
       61 cggcggatcc cgccaggttc cagaaagatg cagacctgac agagagaatc gagcaggtgt
      121 cgatcgtgag tgaccagtat ccccgttccg gtgaagccgt gaagggcttg gtgcagaaaa
      181 tccagcgaat cgcgatccag atgattggtt ggctcgtcga tgagcagaat gtccgggttc
      241 ttccagagcg caacggctat ttgaagccgt ttacgctcgc cgtggctcag agactcccag
      301 cgttcgtgcc agtcggattc gatgtgcagc aggcggcgta aacgagaggc ctccgcgtcg
      361 accgcttcgg caaacgaccg ttgatcgtcc gggggggcgt ccgtgcgctg agcgcagaag
      421 accggaaagc cctcgcgctc gacgcttccc gagtcggctt tcagaacgcc ggcagccagt
      481 tccagcaggc ttgtctttcc tgagccgttc ggaccgatca acccggtcca tcctgcggaa
      541 acatgcagtg aaagatcttc aaagagcgga gttgagctgt ttgcgtgtgt aaacgtaaga
      601 tggtgcagtc gtaacgtcat aattcttcct tcgacggtga aacacaggcc gacgaagacg
      661 cccgaaccac agcaacggaa tcgaacacga aggcaagcac gcagtcaggc gcttctgtcg
      721 gcggaaaatg ggccaacaga cgagatcgct ccaactgtta cttcttcaac gtcgtgctcc
      781 tcaagatgat gttctcagtt tacgcgtttc ctgttccttc tgcaagtgac cgcacgtcgc
      841 ggtttgcttt tgccgggggt gccggatatc ctgaattcag gagccgtggt tggcttgatc
      901 tcagctcagg gagtgaccga gtgaagggta tcgtgaccgg ttgtcagaag acgcagagcg
      961 gctgtttgtg tcgcctgcag ggcccatgcg gcaacatcac cggtctctgg cgaggcgatc
     1021 gaccgccgga gatcggcacg gaatgccgtg ttgagatcac gcttgcactg aacggttcat
     1081 caccccggga gagcgatgtg acagcgcaga cagagtgcga cgacaggagc gggatgaatc
     1141 agctgtccgg ccttgtcgaa caggtgagcg aggatggggt tggcttgctt cgtatggcgg
     1201 cagatgctgt agtgatgctg ccaggagtgc ctgcaagact ggacaagacg cgggtgacga
     1261 tccaggttcc cttcgatcag accgaactgt atcccgagtc gtagctacgc gcgccgatgt
     1321 tctctttcca gcttcagcag cgctgttttg cgatcgactc cgccggcata cccggtgatc
     1381 tgaccgtctg aaccgatgac tcgatgacac ggaatgagga tcgaaatcgg gttgcgtccg
     1441 attgcctgtc cgacggcgcg ggctgacatc gaccgacgac cgagacgctg ggctgcgatg
     1501 tgagctattt gtgcatatgt cctggtttct ccgtagggaa tctcgcg
//
LOCUS       DAORHD010001315         1547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_109948_length_1547_cov_2.973190, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001315 DAORHD010000000
VERSION     DAORHD010001315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109948_length_1547_cov_2.973190"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..875
                     /locus_tag="PKO06_21195"
     CDS             105..875
                     /locus_tag="PKO06_21195"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HNV72237.1"
                     /translation="MDVRVIRIQRRGRSGFSLIELLVVLLIMGLLLVFAIEEYVKHIE
                     QARITKAKADLEMLAKAVRLYGMKEEKTFEVATFTKLYLGNFIGTYLEKDPPNDPWER
                     PYFHRPELGILYSFGEDGVDGQSGRISTDDVVFHYLPSGFYITKAEYIDQNRNNQIDI
                     GDLLEITLSRPGKFLAPTVFDFVASMPTQALGSARVLAASQGQTLQIEFAPPVAPRLE
                     IGKTTIAPREFIDSIVDFSRPPERLASSPAAVIQRRKM"
     gene            1098..>1547
                     /locus_tag="PKO06_21200"
     CDS             1098..>1547
                     /locus_tag="PKO06_21200"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72238.1"
                     /translation="MTTRMFRTGFTLIELLVVITILAVLAGAALPYVQSYVQESRISK
                     AKADLEEIGRALAVYETRERDYNASDVSQLTGRYLNKSPIDPWGRSYVVATSSGIVLS
                     CGPDRVPFNQDDLVYGYQPPLSLVKVKWVDANQTGAVDTQNTPDYLQI"
BASE COUNT          368 a          429 c          404 g          346 t
ORIGIN      
        1 tgaccccgca cctggtgaat ctcgacccgg aaaccccgca gcagagcagt aatctcgttg
       61 tcgggcgtga gggcgaaccg gttctctata agtaggaaaa tggtatggat gtgcgtgtga
      121 tcaggattca acgacggggt cgatcagggt tctctctgat cgagctgctc gtcgtactgc
      181 tgatcatggg cctgctgctg gtgttcgcga ttgaagagta cgtgaagcat attgagcagg
      241 cgcgcatcac caaagcgaaa gccgatcttg aaatgcttgc caaagcagtg cgcctgtatg
      301 gcatgaaaga agaaaaaacc ttcgaggtgg cgacctttac caagctgtat ctgggcaact
      361 tcatcggaac ctatcttgaa aaagatccgc cgaatgatcc ctgggaacgg ccctacttcc
      421 atcgtcccga gcttggcatc ttgtactcgt ttggtgagga cggcgtggat ggtcagtctg
      481 ggcgcatctc gaccgacgat gtcgtctttc attacctgcc ttcgggcttc tacatcacca
      541 aagcggaata catcgatcag aaccgcaaca accagatcga tatcggcgat cttctcgaaa
      601 tcacgctctc tcgccccgga aagttcctgg ctccgacagt cttcgacttc gttgcgagca
      661 tgccgacgca agccctcggt tcggcaagag tcctggctgc gtcacaggga cagactctcc
      721 agatcgagtt tgcgcctcct gtcgctcctc gactcgagat cggaaaaacg accatcgccc
      781 ccagagaatt cattgattcc atcgtggatt tcagccggcc gcccgagcgg ctggcatcga
      841 gtccggcagc ggtgattcag cggcggaaaa tgtaaaacgg gagaagaaaa taaaaatttc
      901 tatatttccc tagggaaata ttggatatcg aggtagaatt acctcgattt ttgtgcgtaa
      961 tgaagcactt tttccgtctt gaaacgggta caagagttgc tgaaataaag atgtatttat
     1021 ttcaccgcag ctcagtcgaa cgaaaaatcg agtaaacagc ggggagaact ggtatgatgg
     1081 gtgtcaggat gaaaaggttg accacaagga tgttccgcac aggctttacc ctcatcgagc
     1141 tgctggtcgt gatcacgatt ctcgcggtcc tcgccggcgc ggctctgccc tacgtccagt
     1201 cctacgtcca ggaaagccgc atcagtaaag cgaaagccga cctcgaagag atcggccgcg
     1261 ccctcgccgt ctacgaaacc cgcgagcgcg actacaacgc ctccgacgtc tcacagctca
     1321 ccggccgcta cctcaacaag tcccccatcg acccctgggg ccgcagctac gtcgtcgcca
     1381 cctcatccgg cattgttctc tcatgtgggc ctgatcgagt cccattcaat caagatgacc
     1441 ttgtctacgg ttatcaaccg cctctatcgc tagtgaaggt gaagtgggtc gatgccaacc
     1501 agacgggtgc tgttgacact cagaatactc ctgattatct ccagata
//
LOCUS       DAORHD010001316         1546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_110083_length_1546_cov_2.606975, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001316 DAORHD010000000
VERSION     DAORHD010001316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110083_length_1546_cov_2.606975"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1546)
                     /locus_tag="PKO06_21205"
     CDS             complement(<1..>1546)
                     /locus_tag="PKO06_21205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72239.1"
                     /translation="TAFQQYQNRQYDAAATAFAQVCQAVPTHHQARQYLAWSLLMAGK
                     TDLARPHVEYLYKLDARNAEYVKLYAAVNTPAASAVQSAGAVQWPVWLEKTARLIETI
                     SEPGDRSMARFDVLLGLNAHPADSSVAERWFVETLKILEGEKSLTREMRYAVYTVGSR
                     LLSSRKWSQAERLASFLLARMESADEDERRRCREFAVESLCLNGSIAQAEQLATTSGL
                     AGALAAARVKVARDAGRLQEAAQLAQAIPPGQQSITLLIDICQRLCRTGQFALVSQLV
                     TGFRKDQRESLAFYVLSELAKLNQTPPEQAAIEKLITTPMWQCRLLCLKARQAHLAQN
                     QSARESFLNKALQLAKENKLTKAPQPVGVLLIECGRASEAMALLPQVDVTDKLWTLLA
                     WMSPLIERGETVWLQKILRESADLFMLERSTLLEEMLRALPPDLLLALQKDLVSAVSP
                     EMRFQIDRHVAAALVLKGRVSEALPALLKSLETIRKDSPEVQAKVVTDFLSATGTRDL
                     ANTSEVL"
BASE COUNT          323 a          480 c          439 g          304 t
ORIGIN      
        1 caacacttcg gaagtgttcg ccaggtcgcg agttccggtc gccgacagga aatccgtcac
       61 gaccttcgcc tgcacctcgg gcgagtcttt cctgatggtc tccagactct tcaggagtgc
      121 gggcaaagcc tcgctcaccc ggcccttcag gaccagggcc gccgcgacgt ggcggtcaat
      181 ctgaaaccgc atctcgggtg aaacggcact gaccagatcc ttttgcagcg ccagcagcag
      241 gtcagggggc aaggctctca gcatttcctc gagcagggtg ctgcgttcga gcatgaaaag
      301 atctgctgac tcacgcagaa tcttctgcag ccaaacggtc tccccacgct cgatcagtgg
      361 cgacatccag gccagcaggg tccagagttt gtccgtcacg tccacctgcg gtaacagcgc
      421 catcgcctca gatgccctgc cgcattcaat gagcagaacc cccactggtt gtggggcctt
      481 ggttaacttg ttctccttcg ccagctgcag agccttattc agaaaggact cgcgcgcgga
      541 ttggttctga gccaggtgcg cctgcctggc tttcaggcac agaagccggc attgccacat
      601 cggggtggtg atcagcttct cgatggccgc ctgctccggc ggcgtctgat tcagtttggc
      661 gagctcagac aaaacataga aggccagtga ctcgcgctga tccttacgaa aaccggtcac
      721 gagctgggac acaagcgcaa actgtccggt acggcacagt cgctggcaga tatcgatcaa
      781 gagagtgata ctctgctgac ccggcggaat ggcctgcgcc agttgcgcgg cctcctgcaa
      841 gcggccggca tcacgggcca ctttcacgcg ggcggcggcc agtgcacctg cgagaccgga
      901 tgtcgtggcg agttgttccg cctgcgcgat gcttccgttc aggcaaagtg attcaacggc
      961 aaactctcgg cagcggcgtc tttcgtcttc atcagcgctc tccatacgcg cgagcagaaa
     1021 ggaggcaagg cgttcggcct gcgaccattt tcggctgctc agcaatcgcg acccaacggt
     1081 atagacggca taccgcatct cacgagtcag tgacttttcc ccctcgagga ttttcaacgt
     1141 ttcaacgaac caacgttccg caacggatga atccgcggga tgcgcattca aacccaacaa
     1201 cacatcgaac cgggccatcg atcgatctcc tggttccgaa atcgtttcga tgagtcgagc
     1261 cgttttctcg agccagacgg gccactgaac ggcgccagcg gactgaaccg cggaggcagc
     1321 gggggtattc acagccgcat agagcttcac atactctgca ttacgcgcat cgagcttgta
     1381 caaatactcg acatgcggcc gagccagatc ggtctttccg gccatcaaca aagaccacgc
     1441 cagatattga cgggcctgat gatgggtcgg aaccgcctga cagacctggg cgaacgccgt
     1501 tgccgccgcg tcatactggc gattctgata ctgctgaaaa gccgtt
//
LOCUS       DAORHD010001317         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_110293_length_1544_cov_4.920752, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001317 DAORHD010000000
VERSION     DAORHD010001317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110293_length_1544_cov_4.920752"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PKO06_21210"
     CDS             complement(<1..437)
                     /locus_tag="PKO06_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72240.1"
                     /translation="MFLCSLVTPVSAARRPLTRLELVELCVSKLQNRFIDEVEAIRCG
                     LLAPYPDGRMHLDWPATRGVAAGVLARLTLTLGLPARPAPFSDVDTTSPLAPHLAQVG
                     ALFPALEEGRFRAEQLAYREDLEQMIESLRTFPEAASPTPRTFR"
     gene            complement(592..>1544)
                     /locus_tag="PKO06_21215"
     CDS             complement(592..>1544)
                     /locus_tag="PKO06_21215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV72241.1"
                     /translation="YRYVYLTDYLMHCFGSPQILAVVAHELGHFRLGHVATYLLFTIV
                     LALATVATKLLVFLHLPDLVMSGWFVSSIIDVGVFLVAFLLVFTALARASERQADRFA
                     SALVGGNLFAETLLQLKEYLVNPDRRLPWWLETHPDFQERIDLARSFTGTTDQMIRQA
                     RVHRTVLAIVGILVALVLIEPVTTIVAFSRASEAIQQGKQPAALQTLTHLETRWGSHP
                     GIIELKGKYAVLQGRWFHALTAAAGLTFGGREHLLPRSEILHHAIAPEVALYFDFMQF
                     LLKTLDLGRVHGVPLLDEVLNLFETVLGHQGILVDLRHDI"
BASE COUNT          382 a          430 c          475 g          257 t
ORIGIN      
        1 cgaaaagtgc gaggagtggg cgaagccgct tccggaaacg ttcgcagaga ctcgatcatc
       61 tgttcgagat cttcccgata ggcgagttgc tccgcacgaa accttccctc ttcgagggcg
      121 ggaaagagcg ctcccacctg agcgagatgc ggagccagcg gcgacgttgt atcgacgtcg
      181 gaaaacggag ccggacgagc cggcagcccg agtgttagag tcaggcgagc cagaaccccg
      241 gcagccaccc cgcgggtagc gggccagtcg agatgcatgc gaccgtcagg atacggtgcc
      301 agcaacccac agcgtatcgc ttcgacttca tcgataaacc ggttctgcag ctttgaaacg
      361 cagagctcga ccagttcgag gcgggtcagg ggacgtcgtg cggccgacac cggcgtgacg
      421 agagagcaca gaaacacgag aagcgctaat gaaatgagcg aactgcgccg atgtatggga
      481 cgacctgaac cacaggatgg aggtttcgcc cccatggccg aactacgagt caagagtcgc
      541 gaccgggacc gtgaagatcc ggatctgcgg attcccaaca atgaagacga atcatatgtc
      601 atggcgcaaa tccacaagta ttccctgatg accgaggaca gtctcgaaga gattgagaac
      661 ctcatcaaga aggggtaccc catgaactcg cccgaggtca agcgtcttca gcagaaactg
      721 catgaaatcg aagtaaagag caacttctgg cgcaatcgca tgatgaagta tctctgaccg
      781 gggaagcaga tgctcccggc ctccgaaggt gaggccggcc gccgctgtca gggcgtgaaa
      841 ccaacgtccc tgaagaacgg cgtatttccc ttttagttcg atgattccgg ggtgagaacc
      901 ccagcgtgtt tcgaggtgcg tcagagtttg cagagccgcc ggctgcttcc cctgctgaat
      961 cgcctctgac gcacgtgaaa aagccacgat ggtggtgacc ggctcgatca gcacgagggc
     1021 gaccaggatt ccgacgatcg ccagaaccgt acgatgtacc cgcgcttgtc gaatcatctg
     1081 atcggtagtg cctgtaaacg accgtgccag atcgatgcgc tcctgaaagt ccgggtgggt
     1141 ttccagccac cagggcagtc ggcgatcggg attgaccagg tactccttca attgcagcag
     1201 ggtctcggcg aaaagatttc ccccaaccag cgctgaggcg aatcgatcgg cctgacgctc
     1261 gctggcacgg gccagtgccg tgaacaccag aagaaaggcg acgagaaaca ccccgacgtc
     1321 gatgatcgag ctgacgaacc agccactcat cacgagatcg gggagatgga gaaacacgag
     1381 cagcttggtg gctaccgtgg ccagagccag cacgatcgtg aagagtagat aggtggcgac
     1441 atgacccaga cggaagtgtc cgagttcatg ggcgacgacc gcgagaatct gcggcgatcc
     1501 gaagcagtgc atcaggtagt cggtgagata cacgtaccga tatc
//
LOCUS       DAORHD010001318         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_110332_length_1544_cov_2.948287, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001318 DAORHD010000000
VERSION     DAORHD010001318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110332_length_1544_cov_2.948287"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PKO06_21220"
     CDS             <1..1001
                     /locus_tag="PKO06_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72242.1"
                     /translation="SHFFDGYKPFFTNTDELFETMCRIADTLGTRDFFVLDENFLDQR
                     ERVERLAQLMEERQRFFHLDIFSSLRTVTAYDPLFLIRLGVQFVWIGIESKHELYDKV
                     KGIDASSVIRRLRDHGISVLVSTMLFLEHHDEHGLREDVDYAIGLEPDFLQFMEYSAL
                     PGTELYQRMKAENRLLTHIPYQEWHGQDRIWFKHPHFSPDQTKQRLDWAFARDFEILG
                     PSLLRIADTRIRPVMMRPPSHDVYLARRILELRDAAREMYPLLPSLVRMAPNAFVRHR
                     AERVAALYDQRLGPMSLAEKVAAGLISLFARMEERRVRLGETTHQPPTFRETYRQR"
     gene            1005..>1544
                     /locus_tag="PKO06_21225"
     CDS             1005..>1544
                     /locus_tag="PKO06_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72243.1"
                     /translation="MQTTRTTLECTRFLALPVAPPADLLTCVAENGWGVLRDLHAPTM
                     DDVRQAARECSGEAKVWLRLFEPTADVVPMLANLDHRPWRGVYLSRGNWDLQEIEALK
                     NAGIPCGLIIQERRDLDLARKHGTLPLLARGNEAGGFNGPLATLVLLQLLCRDAGEPF
                     ILEGGLGPKAAVAAVRAGAA"
BASE COUNT          297 a          422 c          457 g          368 t
ORIGIN      
        1 ccagtcactt tttcgatggg tacaagccgt tcttcaccaa taccgacgag ctgttcgaga
       61 cgatgtgccg tattgccgac accctcggca ctcgcgactt tttcgtgctc gacgagaact
      121 ttctcgatca gcgtgaacgc gtcgaacgcc tggcacaact catggaggaa cggcaacgtt
      181 tcttccatct cgacattttt tcttcgttgc gcaccgtgac cgcgtatgac ccgttgtttt
      241 tgattcgtct tggcgtgcag ttcgtatgga taggcattga aagcaaacac gagttgtacg
      301 ataaggtgaa aggtatcgac gcgtcgagcg tcattcgtcg cctgcgcgat cacggaatct
      361 cggttctcgt ttcgaccatg ctctttcttg aacatcatga cgagcatggg ttgagggaag
      421 acgtcgatta tgccatcggc ctcgagcctg acttcctgca gttcatggaa tattcggcac
      481 tgccgggaac cgaactgtac cagcgcatga aggcggaaaa ccgcctgttg actcacatac
      541 cgtatcagga atggcatggt caagaccgca tctggtttaa acatccgcat ttctcgcccg
      601 accagacgaa gcaacgactc gactgggcat tcgcccgtga ttttgagatt ctgggcccga
      661 gtctgctgcg tatcgcggat acgcgtatca gacctgtcat gatgcggcct ccgagccacg
      721 atgtctatct ggcccggcga attcttgaat tgcgtgatgc cgctcgagaa atgtatccgc
      781 ttcttccttc actcgtacga atggctccga acgcgtttgt gcgtcaccgc gcggaacggg
      841 tagccgccct gtatgaccag cgtcttggtc cgatgtcgct tgccgagaag gtggctgccg
      901 gcctcatttc gcttttcgcc agaatggagg agcgacgtgt tcgccttggc gagacgacgc
      961 atcaaccgcc gacctttcga gagacctatc gtcagaggta accgatgcag acaacacgaa
     1021 cgaccttgga gtgtactcgt ttcctggcgt tgcctgtggc tccgccagca gatcttctga
     1081 catgtgtcgc cgagaatgga tggggggtgc ttcgtgacct gcatgcgcca accatggacg
     1141 atgtccggca ggcggctcgc gaatgctctg gcgaggcgaa ggtctggctg cgtttgttcg
     1201 aaccgactgc tgatgtggtt ccgatgttgg cgaatctcga tcatcgtccg tggcgcgggg
     1261 tctacctctc gcgtgggaac tgggatctgc aggagatcga ggccctgaaa aacgcgggca
     1321 tcccgtgtgg tctgatcatt caggagcggc gagaccttga cctggcgcga aagcatggaa
     1381 cgttgccgct tctggcgcgt ggaaacgaag cgggaggatt caacggtcct ttggcgactc
     1441 tcgtgctttt gcagctgctg tgtcgtgacg cgggtgaacc attcattctc gaaggtgggt
     1501 tgggtccgaa agccgctgtt gcagcggtgc gcgcgggtgc agcc
//
LOCUS       DAORHD010001319         1541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_110682_length_1541_cov_3.763795, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001319 DAORHD010000000
VERSION     DAORHD010001319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110682_length_1541_cov_3.763795"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PKO06_21230"
     CDS             complement(<1..1505)
                     /locus_tag="PKO06_21230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72244.1"
                     /translation="MRQRSESHQHEVERDLVNEAWYFQTEVHPNQALPRLLSGLHEEF
                     GLHPDVPDEQQQRLEAFSRADFERFCRRVMAMTGCRPVCVLFDTVSRTYAMGSGAYLS
                     PQELQSLASILRVLARQFWALWPRDPGARLERQLAERGKAILGHDIDLTKLSERWGGF
                     TSTYRSFGEAFCAVMHGPAVSRRAVYPRLASAFFLFHEFDLNPAFILRQAKTPVHKGM
                     RRSLGWDEPAAVPRFERHDGAQLYVEALRINATQRALVRRYGSHHPGKIPVIRARLAG
                     SRAEPEEAKLVPWLECALGAWLLAGTWVACWLGHRGVPETLSVRRRLAWGFVLALFPA
                     LVGAVLFLGGWLFMRDRLQPLRAMSAVDQQFDRLDAEIAHQIDEKSRWAQSLRWVLPS
                     LDTCSPSEGQAKLTGWLGTSTFDLAFLQFRSGREYLWFPARLRGDPSVIKLFQILMRD
                     FFQTRAGEGESRPDQLLTKAITEQFVDTKNLARWLERGESVQRSPAGSLNQV"
BASE COUNT          356 a          498 c          425 g          262 t
ORIGIN      
        1 acctggttca ggcttcccgc cggtgagcgc tgcaccgatt cacctcgttc aagccagcgg
       61 gcgaggtttt tcgtatcaac gaactgttcg gtaatcgctt tcgtcagcaa ctgatcggga
      121 cgcgactctc cctcaccggc acgtgtctgg aagaagtcac gcatcagaat ctggaacagc
      181 ttaatgacgc tgggatcgcc ccgaagtctg gccggaaacc acagatattc gcgacctgaa
      241 cgaaactgca gaaaagccag atcaaaggtg gaggttccca accagccggt aagtttcgcc
      301 tgtccttctg atggcgaaca ggtatccaga ctcggcagaa cccagcgcag gctctgtgcc
      361 caacgcgatt tttcgtcgat ctgatgcgcg atttccgcat cgagtcgatc aaactgctga
      421 tcgaccgccg acatcgctcg caacggttgc agacgatcgc gcataaacag ccagccaccg
      481 agaaacagga cagctccgac cagagcggga aacagagcca gaacgaatcc ccacgccaaa
      541 cggcgacgaa ccgagagcgt ttcgggcact ccgcggtgtc ccagccagca cgccacccag
      601 gtgcctgcca gcagccaggc acccaaggcg cattcgagcc agggaacgag cttcgcttct
      661 tcaggttctg cgcgcgatcc ggccagtcgc gcgcggatca cgggaatctt tcccgggtgg
      721 tggctcccat agcgccgtac cagggcccgc tgcgtggcat tgatccgcag ggcttcgaca
      781 tacagctgcg cgccatcgtg acgctcgaat cgcggcactg cggcaggttc gtcccacccc
      841 agggaacgcc gcattccctt gtgaacagga gttttcgcct gacggagaat aaacgcagga
      901 ttcaggtcga actcgtgaaa caggaaaaag gcgctggcca gacgcggata caccgctcgt
      961 cgactcactg ccggcccgtg catcaccgcg cagaatgctt caccaaagct ccgatacgtc
     1021 gaggtgaagc caccccagcg ttcagaaagc ttggtcaggt cgatgtcgtg cccaagaatg
     1081 gccttgccgc gttccgcaag ctgccgttca agacgagcgc caggatcacg cggccagagc
     1141 gcccagaact gtcgagccag cacgcgcaga atcgacgcca ggctctgcag ctcttggggc
     1201 gagagataag caccggagcc catcgcatac gtccgagaga cggtatcaaa caacacacag
     1261 accggtcgac atccggtcat ggccatcacg cgccggcaga accgctcaaa atctgcacgc
     1321 gagaacgcct ccaaacgctg ctgctgctcg tccggcacat ccgggtgcaa gccgaactcc
     1381 tcatgaagtc ccgacaacag acgcggtaac gcctggttcg gatgtacctc ggtttgaaag
     1441 taccaggctt cattcaccag atcacgctcc acttcatgct gatgtgactc cgatcgttgc
     1501 cgcatgaact gtcgccacat ctcgtgcaca acaagcagcg a
//
LOCUS       DAORHD010001320         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_110823_length_1540_cov_3.688889, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001320 DAORHD010000000
VERSION     DAORHD010001320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110823_length_1540_cov_3.688889"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..612)
                     /locus_tag="PKO06_21235"
     CDS             complement(64..612)
                     /locus_tag="PKO06_21235"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV72245.1"
                     /translation="MSEDPSRGFDWGPPDAGRFESLKEWGVYALLVLLIGAGIWFLVD
                     AASPGIQPGTLADAFPRATIRPPLETSSAKNTDASQLGHSVSLDGVVTPAGPASLAVP
                     LPAAKPVFLIQLGAFGEEQAALDAQTRLRKKGYVASLTVPDAEYELYRVLLGPFSDEK
                     QADDTARKLNEMDFPCFVIESL"
     gene            complement(605..>1540)
                     /gene="amrS"
                     /locus_tag="PKO06_21240"
     CDS             complement(605..>1540)
                     /gene="amrS"
                     /locus_tag="PKO06_21240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV72246.1"
                     /translation="RCRIAEGRRGRCLGRGREGGTLRLYNYAVVAAANSDPIEKKPLY
                     HFFPGSSILSVGTFGCNLHCRFCQNCDLSQEIQPGEEVLPERLVAVASSHRGNIGVAF
                     TYNEPGIWYEYVVDTAPLLRARGHQVVLVTNGYLEPEPWERLCLVADAMNIDLKAYTD
                     EFYRDVCRGALSPVLANIATAVNKGVHVELTHLVITGLNDRPDDFVRLVEWVAQLDER
                     IPLHLSRYFPRFHETAPATDPALLEEFRRIAARRLRYVYLGNVDTEEGNDTRCPNCGK
                     VWIQRSGYDVSVKHEGPVCVCGQQKHLRMGKSRHV"
BASE COUNT          346 a          478 c          432 g          284 t
ORIGIN      
        1 catggaaacc atggtctcat cagaatagcg cggcttgctc acagacccct ccacgcagcg
       61 aaattacagc gattctatca caaaacaggg aaaatccatc tcgttgagtt tgcgggccgt
      121 gtcgtcggcc tgtttctcat cagagaacgg gcccagcaac acgcgataga gttcgtattc
      181 ggcatctgga accgtcagcg acgccacata ccctttcttt ctcagccgcg tttgcgcatc
      241 gagtgccgcc tgctcttcgc cgaacgctcc gagctgaatc aggaacacgg gcttcgccgc
      301 aggcagcgga acggcgaggc ttgccggtcc ggcgggtgtg accaccccat ccaagctgac
      361 cgagtgtccg agttggcttg catcagtatt ctttgctgat gatgtttcca gtggtggtcg
      421 aatcgtcgcg cggggaaaag catcagcaag agtaccgggc tgtattcccg gcgaggcggc
      481 atcaaccaga aaccagatac ccgcgccgat gagcaacacc agcagcgcat agacgcccca
      541 ttccttaagg gattcaaagc gcccggcatc cggtgggccc cagtcgaaac cgcgagatgg
      601 atcttcagac atgcctgctc ttccccatgc gaagatgttt ctgctgccca cacacgcaga
      661 ccggcccctc atgcttcacc gagacatcat acccgctccg ctgaatccag actttgccac
      721 agttcggaca acgcgtgtcg ttgccttcct cggtgtcgac attgccgaga tacacatagc
      781 gcaagcgccg ggcggcgata cgccgaaact cttcgagaag ggccgggtcg gttgccggag
      841 ccgtttcgtg aaatcgagga aagtaccggg acagatgcaa tggaatccgc tcatcaagct
      901 gagctaccca ctccaccagg cgcacgaagt cgtcgggacg atcattgagc ccggtgatga
      961 ccagatgggt caactcgaca tgcaccccct tgttcacggc tgtggcaata ttcgccagca
     1021 cgggcgacag ggcccctcga cacacgtcgc gataaaactc gtcggtataa gccttgagat
     1081 caatgttcat cgcatctgcc acgagacaga ggcgctccca gggctctggc tcgagatagc
     1141 cgttggtgac cagaacgacc tgatgaccac gtgcccgcaa cagcggtgcg gtgtcgacca
     1201 catactcata ccagatcccc ggctcgttgt aggtgaaagc gacgccgatg ttgccgcgat
     1261 gcgacgaagc cacggccacc agtcgctcag gaagtacctc ttcaccaggc tgaatctctt
     1321 gtgaaagatc acaattctgg cagaagcgac agtgcagatt gcacccaaac gtaccgacgg
     1381 aaaggatgct cgagcccggg aagaagtgat acagcggctt tttctcgatc gggtccgagt
     1441 tggccgcggc aacgacggcg taattgtaca ggcgcagggt gccgccctcg cgaccgcgcc
     1501 cgagacagcg cccacgacgc ccttcggcga tgcggcaacg
//
LOCUS       DAORHD010001321         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111147_length_1537_cov_3.952092, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001321 DAORHD010000000
VERSION     DAORHD010001321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111147_length_1537_cov_3.952092"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1537)
                     /locus_tag="PKO06_21245"
     CDS             complement(<1..>1537)
                     /locus_tag="PKO06_21245"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV72247.1"
                     /translation="PEYHLGVFVPRSYAQHPARSYLTLLNILLVGVFLLTLFFLRELT
                     RWQEEMPLRIFGQLNLALSLSALLPLVGFLLVAWSYSSFHYAQERRMTMRRVEERMQF
                     IDADIRGYREKQIQAYRAARDTIQDQYLDRLSEVKNLLAPVVKNRQASDYILHLADGR
                     VLSTFNGNNPLIDFARAIPKRILMALPIAQPETGTSGAEMRESLKDEIIMKAIEENVP
                     GLMTSMDKQFLLSVSAKENTTIYLDYVTDPRREQPIVGVLAFFLATDIFMQNFFGKLF
                     FSKPMNQATVGLDRIRVVKIDNDANYVFPVGERLTPREQEIFEFCQNTRKSVVWDDTD
                     TDRPWFYGAQFIANEPFVAVVGFDGQKMVQNRRILNAGIAFGVLYALVIFFLTTRFLT
                     MRFAEPLLKLRAAAESVEQQNYTVRVEIAGRDEFNELAGVFNRMVKGLHERQQMSRFV
                     SGDVMEAVTRGDDATQEATRTTVSVLFSDIRGFTELSEKVDAAEIVRMLNAYFTAMES
                     CIVD"
BASE COUNT          377 a          451 c          403 g          306 t
ORIGIN      
        1 gatcgacaat acacgattcc atcgccgtga aatacgcatt gagcatgcgc acgatctccg
       61 cggcatccac cttctccgac aattcggtga agccgcgaat atccgaaaac aacaccgata
      121 ccgtcgtccg ggtcgcctct tgcgtggcat catcgccccg ggtcactgct tccatgacgt
      181 cgcccgagac aaaacgactc atctgctgac gctcatgcaa ccctttcacc atgcggttga
      241 acacgccggc caactcgttg aactcgtcgc gccccgcgat ttcgacccga accgtgtagt
      301 tctgctgttc gaccgattct gccgcagcac gcagtttcaa cagcggctcg gcaaaacgca
      361 tggtcaagaa tcgcgtggtc aaaaagaaga tcaccagagc atacagaacg ccaaacgcaa
      421 tgccggcatt cagaatgcgg cggttctgca ccatcttctg cccatcgaaa cccaccaccg
      481 caacgaaggg ctcattggca ataaactggg ctccgtagaa ccagggacga tcagtatcgg
      541 tgtcatccca gacgaccgac ttgcgggtat tctggcaaaa ctcgaatatc tcctgttcac
      601 gcggcgtgag acgttcgccg acagggaaca cgtagttggc gtcgttatcg atcttcacca
      661 ctcgaatgcg atcaaggcca accgtcgcct ggttcatggg tttactgaag aagagttttc
      721 cgaaaaaatt ctgcatgaaa atgtcggtcg ccagaaagaa cgccagcacg ccgacgatcg
      781 gctgttcacg ccgtggatcg gtcacatagt cgagatagat ggtcgtgttc tctttggcgc
      841 tgaccgacag cagaaactgc ttgtccatgg acgtcatgag gcccggaaca ttctcttcga
      901 tggccttcat gatgatctcg tccttgagcg actcacgcat ttccgctcct gacgtgcccg
      961 tctcgggctg ggcaatcggc agagccatca gaatgcgctt gggaatggcg cgggcaaagt
     1021 cgatcagcgg gttgttcccg ttgaacgtgc tcaagacccg cccgtcggcc aggtgcagaa
     1081 tgtagtctga agcctgacgg tttttcacaa ccggtgccag caggttcttc acctcagaca
     1141 aacgatcgag atactgatcc tgaatggtat ctcgtgccgc tcgataggct tgaatctgct
     1201 tttcgcgata tccgcggata tccgcatcaa tgaactgcat gcgttcttcg acacgccgca
     1261 tggtcatgcg gcgctcctga gcataatgaa agctcgagta cgaccaggca accaacaaga
     1321 agcccaccag aggcaaaaga gcgctgagag aaagagccag gttcagttgt ccaaagatgc
     1381 gcagaggcat ctcctcctgc cagcgcgtca gctcacgcag aaagaacagg gtcagcagga
     1441 aaacccccac cagcagaata ttcagcagag tcagatacga tcgggccgga tgctgcgcat
     1501 aggagcgcgg cacaaagaca ccgagatggt actcggg
//
LOCUS       DAORHD010001322         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111285_length_1536_cov_3.183660, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001322 DAORHD010000000
VERSION     DAORHD010001322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111285_length_1536_cov_3.183660"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PKO06_21250"
     CDS             complement(<1..323)
                     /locus_tag="PKO06_21250"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNV72248.1"
                     /translation="MDEIAKLPPHNLEAETSLLASMMLSREAIEKATENVSSDDFYKA
                     AHREIFSAILQLDQTGTPVDNVTLCEALRQRDQLTQVGGAAYLAQMLNSVPTAANAEY
                     YAKIV"
     gene            complement(470..928)
                     /gene="rplI"
                     /locus_tag="PKO06_21255"
     CDS             complement(470..928)
                     /gene="rplI"
                     /locus_tag="PKO06_21255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNV72249.1"
                     /translation="MQVLLMGDVPRVGRKGETKKVSEGYARNFLFPRKLAVPLTEGTM
                     KNLKLVQVSWAKQAAKEKEAQQELAKKIDGVTLQIKRKAGDKGRLFGSVTSAEISELL
                     HQHSKVEIDKREIITDHIKELGEHEVHIRFSHDAKATLKVVVLPEEDEKK"
     gene            complement(961..>1536)
                     /locus_tag="PKO06_21260"
     CDS             complement(961..>1536)
                     /locus_tag="PKO06_21260"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNV72250.1"
                     /translation="SQLRTVYDKQQVEPATADFLLEQFRGWIDYVALLIPMTFIFGWH
                     SLTLLLLFWAAYRYGGQWGMEVQAPVAFSQWRIDWNFIWVFLAGWLLFNSADAFAPLG
                     LETLSRVAGANFLAISKTIYFVLGFSLLFYYFEVYQVSPPNRVGLSIIATFFYQVPMW
                     LGIADVWLDLRAPKEPQPATRREDDDEDDLF"
BASE COUNT          326 a          453 c          430 g          327 t
ORIGIN      
        1 ttgacgatct tggcgtagta ttcggcattg gccgccgtcg gcaccgagtt cagcatctgc
       61 gccagatagg ctgccccacc cacctgggtc agctgatcac gctgccgcaa cgcttcgcag
      121 agcgtgacat tatcgaccgg ggttccagtc tgatcgagct gcaaaatggc actgaagatc
      181 tcgcggtgcg ccgctttgta aaagtcgtcg ctggaaacat tctcggtcgc tttctcgatg
      241 gcctcgcgac tgagcatcat cgaagccagc agactggttt cggcttcgag attgtgcggc
      301 ggaagcttgg cgatttcgtc catggggtgg ccctctgcgg tgaaaatcaa tcaccccgga
      361 acacgatgcg ccccgacaaa gccggggcgc atcgctgcgt aaccggagtc acagttcaga
      421 acttcggccc ggtacgcggc gtcagccgcg cacaggcacc aaccgttcgt tacttctttt
      481 cgtcttcttc gggtagcacg accactttca acgtggcctt ggcatcgtga gagaagcgaa
      541 tatgcacttc atgctcaccg agttccttga tgtggtcggt gatgatttcg cgcttgtcga
      601 tttcgacctt ggaatgctgg tgcagcagtt cggagatctc ggcgctggtc acggagccga
      661 acagtcggcc cttgtcaccg gctttgcgct tgatctgcag cgtcacgccg tcgatcttct
      721 tcgccagttc ctgctgcgct tccttttctt tcgccgcctg cttggcccag gaaacctgaa
      781 ccagcttgag gttcttcatc gtgccttcgg tgagaggcac cgcgagtttg cggggaaaca
      841 ggaaattgcg ggcatagcct tcactgacct ttttggtctc gcccttgcga ccgacgcggg
      901 gaacatcacc catgagtaga acttgcattg ttatctcctc gaagattggt ttttcgtgat
      961 tcagaaaagg tcgtcttcat cgtcgtcttc acgtcgggtt gccggctgcg gctctttggg
     1021 cgcacggaga tcgagccaga catccgcgat tcccagccac atcggtacct ggtagaagaa
     1081 cgttgcgatg atcgacaggc caacccgatt cgggggactg acctgataga cctcgaagta
     1141 atagaacaac aaagagaacc cgagaacaaa atagatggtc ttgctgatcg ccagaaagtt
     1201 cgccccggcc acgcgcgaca gggtctcaag gccaagcggg gcaaacgcat cggcactatt
     1261 gaacagcagc caacccgcca gaaagaccca gatgaagttc cagtcgatac gccactggga
     1321 aaaggcaacc ggagcctgga cttccatgcc ccactgcccg ccgtagcgat acgcggccca
     1381 gaacaacagc aacagcgtca gactgtgcca gccgaagatg aacgtcatcg gaatcagcag
     1441 cgcgacataa tcgatccagc cacgaaactg ctcgagcaga aagtcggccg tcgccggctc
     1501 gacctgctgc ttgtcgtaaa ccgtgcgcag ctgtga
//
LOCUS       DAORHD010001323         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111305_length_1536_cov_2.728562, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001323 DAORHD010000000
VERSION     DAORHD010001323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111305_length_1536_cov_2.728562"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PKO06_21265"
     CDS             <1..834
                     /locus_tag="PKO06_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72251.1"
                     /translation="VSGVLIPYSPVIGKFVGSAVGAGLFSSVINTAFELRMQKFREQK
                     KSGRDIARTVFKTAMTDAMLAPVTLGLGGGGAAAAGKGLGEMAKEVFKTASVQFLGNA
                     IMTVAGKAMGQQYTAGADALAADAAEATTADGVPPEAFGGQFTGGTIPIMSGPGLTDG
                     ENTVESGTEDPEGSGASSVSIANASRNRDGARGASASEDVQAGTSDDQMRSGSGAEGN
                     GGQDETDPSAGFSREVEHAAWQTLLASRVGVMAALVPRSIPGGVAMHWFVAYAFTGTN
                     R"
     gene            complement(878..1315)
                     /locus_tag="PKO06_21270"
     CDS             complement(878..1315)
                     /locus_tag="PKO06_21270"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HNV72252.1"
                     /translation="MMFRSLKVLFLTVFLFSLPAYSETGEPVQKHPVDQQIEKLEAED
                     PSTHGLIRAYTTGLELWDKELNRVYQALIKALGKDDLAIKALKESQREWIKFRDLEIH
                     WVSLLYGKKDGSMFRPMSAYSIMDITRQRALKLADRLRILSEM"
     gene            complement(1382..>1536)
                     /locus_tag="PKO06_21275"
     CDS             complement(1382..>1536)
                     /locus_tag="PKO06_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HNV72253.1"
                     /translation="NFGSIGIQLGGIGGLAPERRGDLAKLGLRALLAGTFASFMTANI
                     AGVLVD"
BASE COUNT          323 a          389 c          501 g          323 t
ORIGIN      
        1 gtgtcagggg tactcattcc gtattcgccc gtgattggca agttcgtggg gtcggctgtg
       61 ggggcgggtc tgttttccag cgtcatcaat actgctttcg aactgcgcat gcagaagttc
      121 cgcgagcaga aaaagtccgg gcgagatatt gcccgaaccg tgtttaaaac ggccatgact
      181 gatgccatgc ttgcgccggt gactctcggt ctggggggcg gtggtgcggc tgccgccggc
      241 aaaggcttgg gggaaatggc caaggaagtc ttcaagacgg ccagcgtgca gttccttggg
      301 aacgccatca tgaccgtggc gggaaaggcg atgggacagc agtataccgc aggtgctgat
      361 gctctggcgg cagacgcggc tgaagccaca accgccgacg gggttccccc agaggccttt
      421 ggcgggcagt ttaccggagg aacgattccc attatgtcgg gtccggggct gaccgatggc
      481 gagaataccg tcgaaagcgg cacggaagac ccggaagggt caggagcttc ctcggtgtcg
      541 atagccaatg ccagtagaaa tcgtgatgga gcccgagggg cgagtgccag tgaagacgtt
      601 caggccggta ccagtgacga tcagatgaga tcgggatcag gtgcagaagg gaatggtgga
      661 caggatgaga cggacccatc tgccgggttc tcgcgtgagg tcgaacatgc cgcgtggcag
      721 accctgctgg cgagccgggt cggggtcatg gcggcgctcg tgccccgatc gattcccggt
      781 ggcgtagcca tgcactggtt cgtggcgtat gcctttaccg gaacgaatcg ttagaatcct
      841 gacggtaagc tgatcgcaga atctttggct cggacgctca catctccgac aaaatgcgca
      901 gtcgatcggc gagcttcagg gcgcgttgac gggtaatatc catgatgctg taggcggaca
      961 ttgggcggaa catgctgccg tctttcttgc catagagcag cgacacccaa tgaatttcga
     1021 gatcacgaaa cttgatccac tctcgctgtg actctttgag cgctttgatc gcgaggtcat
     1081 ctttgccgag agccttgatg agagcctggt agactcgatt gagttctttg tcccacagtt
     1141 cgagtcccgt cgtataggcc cgtatcaacc cgtgtgtcga ggggtcttcg gcttcgagtt
     1201 tttcgatctg ttggtcgacc gggtgctttt gtactggttc tccagtttca gaatatgctg
     1261 gtagagagaa aagaaaaaca gtgagaaaga ggactttgag actgcgaaac atcatcactt
     1321 ctcctggtga aaaatggaaa gccggtcgaa tcacgagatc cgaccggctt tgcgtgagag
     1381 atcaatcgac gaggacgccg gcaatgttcg cggtcatgaa gctggcaaac gttccggcca
     1441 gcagggcgcg gagtccgagt ttggccaggt cgccacggcg ttccggagcg agtccgccga
     1501 tgccgcccaa ctgaatgccg atcgaaccga agttcg
//
LOCUS       DAORHD010001324         1533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111620_length_1533_cov_3.502706, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001324 DAORHD010000000
VERSION     DAORHD010001324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111620_length_1533_cov_3.502706"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1161
                     /locus_tag="PKO06_21280"
     CDS             373..1161
                     /locus_tag="PKO06_21280"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV72254.1"
                     /translation="MMVTRTGTLFSANQINEAQNRESKALSRIATGKQILAAADDPAA
                     LSVTMTLEAMTRGLSMQINNRQDEISLIQTAEGALGTLSEMTQRIRELSVQASNGTLT
                     DSDRQNIQFEIDQLNQQADQIANGTEFNTKKLLDGSLNLQLQNGNSLAIPPFDAANLG
                     LTQVDVRTQSGASSSIGYADQALDRVTTQRSSLGAIQNGIAAEVRNLTDEFLNTIAAN
                     SRISDADIAREIVNLTNSQVQGQAATSAFRMDNAARTRVLQLLG"
     gene            complement(1243..>1533)
                     /locus_tag="PKO06_21285"
     CDS             complement(1243..>1533)
                     /locus_tag="PKO06_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase"
                     /protein_id="HNV72255.1"
                     /translation="EQIRYAADGRVLTGVNAYKVPDITFVPPHFDITLLPNAANPFAV
                     CNSKAIGEPPFVHGIGAYLALLEALRSVRRDKEPPSLPLTPEKVFMYLHSQS"
BASE COUNT          319 a          446 c          432 g          336 t
ORIGIN      
        1 agaaggcgat gtgccggctg tttccgaaga gcccgtcgcc tctgctgaag ccgcggtcct
       61 ggtgccggtg gcgtctgaat cgggctcagc ctcagtgccg gcttcgctcg ccggcccggc
      121 taccgtatca gagtcagttg tcactcactg agcctggtgc cgcgtcaccc gacgccccct
      181 gggcgaccac gagacatcga attgtaacct gataccagga tacggtatcg atccgagcct
      241 tcctgtttgt caaaaaaacg tttgttggtt tttgttgtgt tctgtgtgtg aatgtttgtt
      301 tacgtccatg ctgttctttc tacgtggact ttcttgcctc tgataggtat attctgaaat
      361 aggagggccg atatgatggt cacacgaaca ggcaccctat tcagcgccaa tcagattaac
      421 gaggctcaga accgcgagag caaggctctg tcgcgcattg ccaccggcaa gcagattctg
      481 gcggcggccg atgatccggc ggctctctcg gtgaccatga cgctcgaagc catgactcgt
      541 ggcctcagca tgcagatcaa caaccggcag gatgagattt ccctgattca gaccgccgaa
      601 ggagccctcg gaaccctcag cgagatgacc cagcgcattc gagaactttc agttcaggct
      661 tcgaatggca ccctgaccga cagcgatcga cagaacattc agttcgagat cgatcagctg
      721 aatcagcagg ccgatcagat cgcgaatggc accgaattca acacgaaaaa actgctcgat
      781 ggttcgttga atctgcagct tcagaatggc aattcattgg ccattccccc gttcgacgcg
      841 gccaacctcg ggctgaccca ggtcgatgtg cgcacgcaga gcggggcttc aagctccatc
      901 ggctatgctg accaggctct cgatcgcgtc acgacccagc gctcgagtct gggagcgatt
      961 cagaacggca ttgctgccga ggtgcgcaat ttgaccgatg agttcctgaa cacgattgcg
     1021 gcgaacagtc gcatctcgga tgcggatatc gcccgcgaga tcgtgaacct gaccaacagc
     1081 caggtacagg gccaggcggc aaccagcgcc tttcgcatgg ataatgccgc gcgaacgcgt
     1141 gtgctgcagc tgctcggcta gacgtattcc gcctctcgtt cgctttcgat gaaaaagggc
     1201 cccgttcggg gccctttttc atcgattcgg cttttgcctt cgtcagcttt ggctgtgcag
     1261 atacatgaac actttctcgg gtgtcagtgg cagcgaaggc ggttctttat cccggcgaac
     1321 gctcctcaaa gcttcgagca atgccagata ggccccgatt ccatggacga aagggggctc
     1381 gccgatcgcc ttactgttgc agaccgcgaa tggattcgcg gcgttgggca gcagggtgat
     1441 atcgaaatgg ggcggcacga acgtgatatc cggaaccttg tacgcattca cccccgtcag
     1501 cacccgaccg tcagcggcat atctgatctg ctc
//
LOCUS       DAORHD010001325         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111738_length_1532_cov_4.304672, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001325 DAORHD010000000
VERSION     DAORHD010001325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111738_length_1532_cov_4.304672"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PKO06_21290"
     CDS             complement(<1..1114)
                     /locus_tag="PKO06_21290"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV72256.1"
                     /translation="MSDRDSPTDFLIVRGVSEHYLKHFSVRVPHDRLTVVTGISGSGK
                     SSLVFDTICREGQRRFLQTLSTHARQMLGTMKPPVCESIEGLRPTLAVAQQGRSSHAR
                     STVGTMTDLHDALRLLFARLGVAYCPSCHLPYISSTAESLLDIIINTTVGKPVRLLAP
                     VILGHRGSFRRELEDLRHQGLLAARIDGVLTPLMPTPKLTAKMLHGIEAVVADPLPSD
                     PSEALALVQKALQIGKDVLLYQIETETPQRLSRRHACSQCGGSPPEIGPRLFSFNSAY
                     GACPACQGLGLEDAIDPDLLIGDDTRTVREGALVLTTPKGYIIYSQVTMDVLDQVCRA
                     HGFTVDVPWRDLTDEQRRVILYGSDRLKIPFGKRPLE"
     gene            complement(1231..>1532)
                     /locus_tag="PKO06_21295"
     CDS             complement(1231..>1532)
                     /locus_tag="PKO06_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72257.1"
                     /translation="IVDQKTITAVLEKMVLFMNKDAVNAASGLQDLKYYQNPKAAMKL
                     YANKLRPELFLLEWKGLGYTREERLRVPPGWADMKDVTLEESEDFINNLDLSPRK"
BASE COUNT          319 a          412 c          491 g          310 t
ORIGIN      
        1 actcgagcgg gcgcttgccg aacgggatct tgaggcgatc tgagccgtac aagatgaccc
       61 ggcgctgctc gtcggtgagg tctcgccacg gaacgtcgac ggtgaagcca tgagcgcggc
      121 agacctggtc gagaacgtcc atcgtgacct gagaatagat gatataccct ttcggtgtcg
      181 tgagaacgag agcgccctct cgtacggttc gcgtgtcgtc accgatcagg aggtccgggt
      241 cgatcgcgtc ttcgagtccg agtccctgac aggccggaca cgctccgtag gcactgttga
      301 acgagaagag acggggaccg atctcgggcg gactgccgcc gcattgtgag caggcatggc
      361 gacggctgag gcgctgaggt gtttcggtct cgatctgata caacagaacg tctttgccga
      421 tctgcagggc tttttgtacc aaggccaacg cctctgacgg gtcggacggc agaggatcgg
      481 caacaaccgc ttcgataccg tgaagcattt tggcggtgag cttcggcgtg ggcatcagcg
      541 gcgtcagtac cccgtcgatg cgtgcggcca gaagaccctg atggcgcaga tcctccagtt
      601 cccggcggaa actgccgcga tgtcccagaa tgacgggtgc cagcaggcgt accggtttgc
      661 cgaccgtggt gttgatgatg atatcgagca gagattccgc ggttgaagag atgtatggaa
      721 gatgacaact cggacaatat gcgacgccga gccgagcgaa caaaaggcga agagcatcgt
      781 gaaggtcggt catggtgccg accgtcgaac gggcgtgtga acttcggccc tgctgggcga
      841 ccgccagcgt cggtcgcagt ccttcgatac tctcgcaaac gggcggtttc atcgttccga
      901 gcatctgacg ggcatgagtc gaaagcgtct gcagaaaccg ccgctgacct tcgcgacaga
      961 tggtatcgaa caccagagac gatttgccgg aaccggaaat gcccgtcacg accgtcagac
     1021 gatcgtgtgg aacccggacg ctgaagtgtt ttagatagtg ctcggatacc ccgcgcacga
     1081 tcagaaaatc agttggtgaa tctcgatctg acatgcggca agggtagcct gtttcggccg
     1141 gtgagacaat cccccgagcg tgggcacaaa cccgcgtaaa ggcgaaaccc ctcggcgatg
     1201 ccgaggggtt tcgtgatgaa cggcggacgg ctacttgcgg ggagagagat cgagattgtt
     1261 gatgaaatct tcagattctt cgagagtgac gtccttcatg tccgcccagc cggggggcac
     1321 tctgagacgc tcttcgcggg tatagccgag acccttccat tcgagcagga agagttccgg
     1381 gcgcaacttg ttggcgtaca gtttcatcgc cgctttgggg ttttgatagt atttgagatc
     1441 ctgcaggccg ctggctgcgt tgaccgcgtc tttgttcatg aacagaacca tcttctcgag
     1501 gacggcggta atcgtcttct ggtcgacaat ct
//
LOCUS       DAORHD010001326         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111750_length_1532_cov_3.602573, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001326 DAORHD010000000
VERSION     DAORHD010001326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111750_length_1532_cov_3.602573"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKO06_21300"
     CDS             complement(<1..621)
                     /locus_tag="PKO06_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72258.1"
                     /translation="MTGPIHGDLLNRLFQVQRDILSAAKDTDALLAILSQGCRTIIGG
                     AAFGLLFYDSGTKSSRVRFNDGFANSRLDEFVKRFDDARTEGETFQALTIPVDGGFIE
                     HLLPLTNSKGESYGVMLFKLPKDAPVLAEYSEICSDALSRTMEMEINRLQMAELATLL
                     EVGKAVSSTLDLTELLKKIMAMATKVMRCETATVYLVDKKTRELYFN"
     gene            complement(631..>1532)
                     /locus_tag="PKO06_21305"
     CDS             complement(631..>1532)
                     /locus_tag="PKO06_21305"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72259.1"
                     /translation="QGSWRYLPEALLMLLSLWTLWAQSLPSVWKGVGVALFGYGGLFT
                     LSVVLFTAGYSMSCALWGLGFPLILAAVYIVGYFSEGREKAKIRELFGKQVSPGVVQE
                     LLRDPERVLAPKRHEISICFVDVCGFTSFSEKHSADRVLVQLNFYFSHLVRIVHQYGG
                     TLDKFIGDAMMITTGVPLFDANHALNMIKLALEIRRQVGQINTNLPAGLEPFQVSCGI
                     NSGEAILGNVGAAERMEFTVIGDTVNVASRLQGKSGPMEIVVGSRTHDLVKDYAIMEP
                     LEPVTVKGKSTPIQAFRLVGLKE"
BASE COUNT          392 a          440 c          374 g          326 t
ORIGIN      
        1 gttgaaatag agttcacggg tcttcttgtc gacgaggtaa accgtagccg tctcacagcg
       61 catcaccttg gtggccatgg ccatgatctt cttcagcaac tcggtcaaat caagggttga
      121 gctgactgcc ttgcccactt cgagcaaagt cgcgagctca gccatctgca gacggttgat
      181 ctccatttcc atggtgcgag ataacgcatc ggagcagatc tcggaatatt cagccaggac
      241 cggcgcatct ttcggcagct tgaacagcat gaccccgtaa gattcgcctt tgctgttcgt
      301 cagcggaaga agatgttcga tgaacccacc gtcaaccggt atcgtcagtg cctgaaaggt
      361 ttcgccttca gtacgggcat catcaaagcg tttgacgaat tcgtcgagtc gggaattggc
      421 aaaaccgtca ttgaagcgaa cgcgggaaga cttggtgcct gaatcgtaaa aaagcagacc
      481 gaatgccgca ccgccaatga tcgtgcgaca gccctgactg agaatggcca gcaaggcatc
      541 ggtatctttg gcggcagaga gaatatcacg ctgcacctga aacaggcggt tcagcagatc
      601 tccgtgaatt ggcccggtca cttcggcttc ttactccttc aaacccacca ggcgaaacgc
      661 ctggatcggt gtgcttttcc ccttgaccgt caccggctcg agaggctcca tgatcgcgta
      721 gtccttgacc agatcatgcg ttcgcgaacc aacgacaatc tccatcggtc ctgacttccc
      781 ttgcaaacga cttgctacat taaccgtgtc accgatcacg gtaaactcca tgcgctcagc
      841 agcgccgacg tttccgagaa tggcttcacc actgttaatg ccacaactga cctgaaaggg
      901 ttcaagacca gccggaagat ttgtattaat ttgccctacc tgacgtcgaa tttcaagcgc
      961 taattttatc atgttcaggg catgattggc atcaaaaagt ggaaccccgg tcgtaatcat
     1021 cattgcgtca ccgataaact tgtccagagt cccgccatac tgatgtacaa tccgaaccag
     1081 atgggaaaaa tagaagttga gttgaaccag cactcggtca gcgctgtgtt tttccgaaaa
     1141 cgaggtaaac ccgcacacat cgacgaaaca gatggaaatc tcgtggcgtt tgggcgcaag
     1201 aacccgctca gggtctcgca acaactcctg tacaaccccc ggcgacacct gttttccgaa
     1261 cagttcgcga atcttggcct tctctcgacc ttccgaaaag taccccacga tatatactgc
     1321 cgccagaatc agtggaaacc caagacccca gagggcacag ctcatcgagt atccggcagt
     1381 gaacagaacg acactcaggg tgaacagacc gccatatccg aacagcgcca cgcccacgcc
     1441 cttccacacc gaaggaaggc tctgcgccca taaggtccag agggataaga gcatcagcag
     1501 tgcctcggga aggtatctcc atgacccctg at
//
LOCUS       DAORHD010001327         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111879_length_1531_cov_4.388889, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001327 DAORHD010000000
VERSION     DAORHD010001327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111879_length_1531_cov_4.388889"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PKO06_21310"
     CDS             complement(<1..233)
                     /locus_tag="PKO06_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72260.1"
                     /translation="MDHQLDARDPSFAERKDLRFFNLLQGVAADALESLWLFNTADEE
                     FEVANETISGLRGGTEDDSLERGGVFSGKCQNN"
     gene            264..>1531
                     /locus_tag="PKO06_21315"
     CDS             264..>1531
                     /locus_tag="PKO06_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72261.1"
                     /translation="MAEIEALGSSSEKLLFDLKLVQLWQRSQSVPPATPSQDLPNHAQ
                     GAYAQQPHHDSSGWIEGYQEAGFAPVFVHAAATETRAITVFKRIGSGTLAVDMQNFGH
                     RDQAQMRWKFFNLCDLPHPLLKTINGTCAGGMGAPERQLVPLPAGRYLLQITAADGQG
                     SPDSYLLTARFPVASQPLPLVLCGTMPDEGMSGIHPECPIRLTFNQPVAESSFRPDQI
                     TITGEQSGVLPWQHQFLTDEHAILLRLRSPAHFADGEQVSLRVGSQAITSRDGTLLGT
                     DTSVNFRIGRTIRTDARLTLQRRNDGAMGIGRHEFTVTSSHPLRQAPALTCITADGRK
                     SVMGMSPSGALTWQGSLDITAETCQGKARITATAVAENGDPLPAPLPLMVFIDTQPPV
                     APIAAPIARAVAGGQVEVSWAPPPDRQDITG"
BASE COUNT          310 a          494 c          427 g          300 t
ORIGIN      
        1 aaattgttct ggcacttccc actgaaaaca cccccccgct ccagcgagtc gtcctccgtt
       61 ccgccgcgca gaccgctgat cgtctcattc gcgacttcga actcttcatc agcagtgttg
      121 aacaaccaga ggctttcgag cgcatcggca gctacaccct gcagcagatt gaagaaccgc
      181 aggtcttttc gttcagcgaa cgacgggtcg cgcgcatcaa gttggtgatc cattccaact
      241 ggggttcacg ggatgaaacg cagatggctg aaatcgaagc cctgggatcc tcttcagaaa
      301 agctgttatt cgatcttaag ctcgtacaac tgtggcaacg cagtcagagc gttcctcctg
      361 ccacgccttc acaggatctg ccaaatcacg cgcagggagc gtatgcccaa cagccgcatc
      421 acgattccag cggttggatc gaggggtatc aggaggcggg cttcgctccc gtcttcgtgc
      481 atgcagctgc caccgaaact cgcgccatta ccgtgttcaa acggatcggg tcaggcactc
      541 tcgccgtcga catgcagaac ttcggccatc gggatcaggc gcagatgcgc tggaaatttt
      601 tcaacctctg cgacctgcca cacccgctgc tgaaaaccat caacgggacc tgtgccggcg
      661 gcatgggagc tccggagcgc caactcgtcc cactgccggc tggtcgttat ctgctgcaga
      721 tcacagccgc cgatgggcaa gggtcgcccg acagttatct tctcaccgcg cgcttcccgg
      781 tcgcatcaca accactgccg ctggtgctgt gtggaacgat gcccgatgaa ggcatgtccg
      841 ggatccatcc cgagtgtccg attcgcctga cattcaacca accagtcgct gagtcatcgt
      901 tccgtcctga ccagatcaca atcaccggtg agcagagcgg cgttcttccc tggcagcatc
      961 agttcctgac tgacgaacat gcgattcttc tgcgactgcg ttcgccagcg cattttgctg
     1021 acggagaaca ggtctcgctt cgcgtcggat ctcaggcgat caccagccgg gatggcacct
     1081 tgctgggaac ggatacatcg gtgaactttc gcatcggccg aacgattcgc acggacgcgc
     1141 ggctgacatt gcaacgtcga aacgacggtg ccatgggcat cggacggcat gagttcacgg
     1201 tgaccagttc acaccccttg cgtcaggccc cagctctgac gtgcatcacc gccgatggtc
     1261 gcaaatcagt catgggaatg tcgccctcag gggcgctgac gtggcaaggg agcctggaca
     1321 tcacggcgga aacgtgccag ggtaaagccc gcatcacggc caccgccgtg gcagaaaacg
     1381 gtgacccgtt gccggcacca ctgcctctga tggtcttcat cgacacccag cctcccgttg
     1441 cgccgatcgc ggctccgatc gctcgagccg tcgcgggtgg acaggtcgaa gtctcctggg
     1501 ctccgccacc ggatcgccag gacattaccg g
//
LOCUS       DAORHD010001328         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111912_length_1531_cov_3.004743, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001328 DAORHD010000000
VERSION     DAORHD010001328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111912_length_1531_cov_3.004743"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKO06_21320"
     CDS             complement(<1..257)
                     /locus_tag="PKO06_21320"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72262.1"
                     /translation="MPVSIDDRDLANQHFDRGLSFDIRGKLDDAINEMEKAVSCDPEF
                     AEAYNKLGDFYQKKGWIPKAVDMYKKSSQLKPEVENSHFDLG"
     gene            complement(418..1497)
                     /locus_tag="PKO06_21325"
     CDS             complement(418..1497)
                     /locus_tag="PKO06_21325"
                     /inference="COORDINATES: protein motif:HMM:NF003676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNV72263.1"
                     /translation="MRRALSVVVLVVVALVSCSLRADAESQVRLKDISRLSGVREYQL
                     LGYGLVVGLDGTGDKTPMSIEMVRGMLQNMGMEIDKTSIQSKNVAAVVVSAMLPAFGR
                     AGESFDVTVSSIGDAKSLQGGVLLPVLLKGGDGNVYAVGQGHVSVGTIAGGGGAGAGA
                     TARGGHLTVARVPNGAILEREVGDSFAHDGRFALLLNQKDPALARRVKEAVDRKYGDG
                     WARTTDPGSVEIVIPSSFKEDPVSFAATIMGLSIAAEESNAVVINERTGTVIVGNRVR
                     ISKVTISQGGLKIAVEPPAGQKPKTGAAAKDEPQPSMISLQGETTVDDLVKALNAVGA
                     TPKDLVSIFQAIQAAGALHGDLKVM"
BASE COUNT          336 a          492 c          361 g          342 t
ORIGIN      
        1 cctaagtcga aatgggaatt ctcaacctca ggcttcagct gactggattt cttgtacata
       61 tcgacggctt ttgggatcca gccttttttc tgatagaaat cgccaagctt gttgtaggct
      121 tccgcaaatt cggggtcaca actgacggct ttctccattt cgttgatcgc gtcgtcaagc
      181 ttgccacgaa tatcgaagga aaggccccgg tcgaaatgct gattggctaa atcccgatcg
      241 tcaatgctta cgggcatggt gctttctcct gagggtaatc gaggcgcttg tgaggggggc
      301 ccacaaccgc atctgcgaag cgtgggcaga cagagaattc tgaatcaccc tatcacataa
      361 gttgtcacag atcaagtatt tccataccgg aaaatatcgg gaactgaccc ggcagactta
      421 catgaccttc agatctccat ggagagcccc ggcagcctga atcgcctgaa agatagaaac
      481 cagatctttt ggggtcgctc cgaccgcatt caaagctttc accagatcgt ccaccgtggt
      541 ctcgccctgc agactgatca tgctcggttg cggctcgtcc ttcgccgcgg ctcccgtttt
      601 cggcttctgc ccggccgggg gctctaccgc gattttcaag ccgccctgag agatcgtcac
      661 cttgctgatg cgcacccgat tcccgacgat gacggttcct gtgcgctcat taatgaccac
      721 cgcgttcgat tcttcggcgg caatactcaa ccccataatc gtcgcggcaa agctcaccgg
      781 atcttctttg aaactgcttg gaatcacgat ttcaacggaa cccggatcgg ttgtcctggc
      841 ccatccgtcg ccgtacttcc gatctacggc ctcttttacc cgacgcgcca atgccgggtc
      901 tttctgattc agcaacagcg caaagcgacc gtcgtgcgca aacgaatccc cgacttcgcg
      961 ctccagaatc gccccgttgg gaacccgcgc gaccgtcaaa tgccctccgc gagccgttgc
     1021 gccggcaccc gcaccaccgc cacccgcaat ggttccgaca ctgacgtgac cctgacccac
     1081 ggcatacaca ttgccatcgc cgcccttcag taagaccggc agcagcactc caccctggag
     1141 gcttttagcg tcaccgatcg aactgacggt cacgtcaaag ctttcgccag cacgcccgaa
     1201 agccggcaac atcgcactca cgacaaccgc cgccacgttc ttgctctgaa tcgaggtctt
     1261 gtcgatctcc atgcccatat tctgcagcat gccgcgcacc atttcgatgc tcatcggcgt
     1321 cttgtcgccc gtcccgtcga gaccgacaac aagtccatac cccaagagct ggtattcacg
     1381 cactccactg agacgggaaa tgtctttcag ccgcacctgc gattcggcgt cagcccgtaa
     1441 cgagcaagaa accagagcga caaccaccaa cacgacaaca gagagtgccc gcctcattta
     1501 ttcaccattt gataatattg ttattatttt a
//
LOCUS       DAORHD010001329         1531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_111922_length_1531_cov_2.915989, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001329 DAORHD010000000
VERSION     DAORHD010001329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111922_length_1531_cov_2.915989"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKO06_21330"
     CDS             complement(<1..600)
                     /locus_tag="PKO06_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV72264.1"
                     /translation="MVESNHLLSDDRAKRVPHLSPEAALLKHWGYSTFRPLQREAVSA
                     ALTGRDALVVLPTGGGKSLCYQLPAACGVGLVVVISPLIALMDDQVMAACESGLRAGA
                     LHSAASEATRRRVWSALQRGDLDLLYVSPERLVVSELLESVRARLALLAVDEAHCVSH
                     WGHEFRPEYRQLGSFFSAFPAVPRLALTATATPAVQKDIC"
     gene            complement(741..>1531)
                     /locus_tag="PKO06_21335"
     CDS             complement(741..>1531)
                     /locus_tag="PKO06_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72265.1"
                     /translation="DAMRLRATPFSDEVHDFIERHDVIYVIEQNRDAQMRSILINELE
                     IDPFKLVPILNFDGMPITAEAIVKQVIEMGVSPESAESVVSSMYDEIVKQAREELFTP
                     DSLVPAILGGLAVAVVGGGIWAGVVVATNYEIGYLAWGLGLAAGWGVVKASGGKKGLI
                     PQLIAVASGLGGILFGKYFIFYHFFKQALVKQLADQPGGAEAIKEFTLLSGQFIKVFF
                     QNLSAMATGHDALWIILAVVTAWSMAKAVNLPTAVLHAPPRERF"
BASE COUNT          359 a          494 c          415 g          263 t
ORIGIN      
        1 gcagatatct ttctgcacgg cgggggtcgc ggtcgccgtc agagccagac ggggaacggc
       61 gggaaaggcc gagaagaagc ttcccagctg ccgatactca ggacggaact cgtggcccca
      121 atgggacacg caatgcgctt catcgacggc cagcagcgcc agacgcgcgc gaacagactc
      181 gagaagttca ctcacgacca gtctctccgg gctgacatac aggagatcga ggtcaccccg
      241 ttgcaacgcg ctccacacac gacgacgcgt ggcttcagag gccgcggaat gcagagctcc
      301 agctcgcaag cctgattcac aggcggccat cacctgatcg tccatcagag caatcagcgg
      361 cgagatgacc acgaccagac cgacaccgca cgcggccggc agctgatagc agagactttt
      421 tcccccgccc gtcggcagca ccacgagcgc atctcgacca gtcagagccg ccgacaccgc
      481 ttctcgctgc aacggacgaa aggtcgaata cccccagtgc ttcagcaggg ccgcttcggg
      541 cgacaggtgc ggcacccgtt tcgcacggtc gtcagacagc agatgattcg actcaaccat
      601 gctccgactg tatcacagcc tgatgccgca cggaagatcg gacaggcaag caggaacgcg
      661 atgccccacg attctccgga ctaacccgct ccaatcatcc tgagcggtga ctcatcgcag
      721 caggagacga aacaggctat ctagaaacgc tctcgtggcg gcgcatgcag taccgccgtg
      781 ggcagattca ccgccttggc catgctccag gcggtcacga cggccagaat gatccagagc
      841 gcatcatgcc ccgtggccat cgcgctcagg ttctggaaga agactttgat aaactgcccc
      901 gaaagcagcg taaactcctt gatcgcctcc gccccgccgg gttgatcggc cagctgctta
      961 accaatgcct gcttgaagaa gtggtaaaag atgaaatact ttccgaacag aattccccca
     1021 agaccggaag cgacggcgat gagctgagga atcaacccct tcttgccgcc agacgctttc
     1081 acgactcccc agccggcagc cagaccaagc ccccaggcga gatacccgat ttcatagttc
     1141 gtggcgacca ccacgccagc ccagattccg ccacccacga cggctaccgc caggcccccg
     1201 agaatcgctg gtacgagtga gtcaggagta aaaagctctt cacgagcctg tttcacgatc
     1261 tcatcataca tcgaactgac gacggactca gcgctctccg gcgacacccc catctcgatc
     1321 acctgtttca caatcgcttc ggcggtgatg ggcattccgt caaaattcag aataggtacc
     1381 agtttgaacg gatcgatttc cagttcgttg atcagaatgg aacgcatttg tgcgtcgcgg
     1441 ttctgttcga tgacgtaaat cacatcgtgg cgttcgataa agtcgtgcac ttcgtcggaa
     1501 aacggtgttg cccgcaggcg catggcatcc a
//
LOCUS       DAORHD010001330         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_112277_length_1528_cov_2.985743, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001330 DAORHD010000000
VERSION     DAORHD010001330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112277_length_1528_cov_2.985743"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PKO06_21340"
     CDS             <1..650
                     /locus_tag="PKO06_21340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72266.1"
                     /translation="RVRTVIVAAIVISGVVRFADSQLLEGMDLLRLVAIAGSAILLAV
                     LFPCSLLLWLWYRKTPEVPGYRSAARGFKGCVFFITCILMPSILDVPDHMRVFYHIGW
                     CEAYVNKLEEFRAREGRYPISKQEAREKISIVPTLRVKLASTSYSILIPLRQKTQRLS
                     LDGPPAHWEYADSMNSDRPAMSIPVGMMKGFPEHLATYGPSMRPFSSRNDQEDYE"
     gene            678..1223
                     /locus_tag="PKO06_21345"
     CDS             678..1223
                     /locus_tag="PKO06_21345"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV72267.1"
                     /translation="MSAPQTPAPPVPGLTGLSAFTMLVFGFIFTLIGGYVFSLSWEGW
                     ITYFRAGNWRSVSARVESAKLAVHSNGTRAKFYSLHTVYHYTWQGQDMVASRSLPTYG
                     PSGMSGSGEFYREKLNYLNVQMQASQPVDILVNPDNPTESLLIREVCPGMFFSIIFGG
                     IMFTFGFFGLLGLLFPSLRLL"
     gene            1269..>1528
                     /locus_tag="PKO06_21350"
     CDS             1269..>1528
                     /locus_tag="PKO06_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72268.1"
                     /translation="MRASEFESIDHVPPVDWKDAPQEVRDALNALKEGRDAPDVFFLK
                     SDLTQTQKERRITIAWACTIFVAVFGLGVCAPLALKQSIQGGL"
BASE COUNT          313 a          413 c          417 g          385 t
ORIGIN      
        1 tgagggtcag aactgtcatt gtcgctgcga ttgtcatcag cggcgtggtg cgtttcgcgg
       61 acagccagct tctggaaggc atggatctgc tgcgactggt tgcgattgcc ggttccgcga
      121 tccttctggc ggtgctcttt ccctgttcac tgcttctctg gctctggtac cgcaaaacgc
      181 cggaagttcc tgggtatcgg tcagccgcca gagggttcaa agggtgtgta tttttcatca
      241 cctgtatcct catgcccagc attcttgatg ttcccgacca catgcgcgtc ttctatcaca
      301 tcggttggtg tgaagcctac gtcaataagc tcgaggagtt tcgtgcgcgt gagggtcgct
      361 acccgatttc caagcaggaa gcccgcgaga aaatctcgat cgtaccaact ctgcgcgtca
      421 aactcgcttc cacctcatac tcgatactga ttcctttgcg gcagaaaacg cagcgtctgt
      481 cgctcgacgg tcctccggcg cattgggaat atgctgactc gatgaattct gatcggcctg
      541 cgatgagcat tcccgtgggc atgatgaaag gcttcccgga gcatctcgcc acgtacggcc
      601 caagcatgag accgttcagt tctcgaaacg accaggagga ctatgagtag tcctgtcgtt
      661 ggcatgaggt catctccatg agtgctcccc agactcctgc cccgcccgtt cccggtctga
      721 ccgggctcag cgcttttacc atgctggtgt tcggattcat cttcaccttg atcggaggat
      781 acgttttttc tctttcatgg gaaggttgga tcacctactt tcgcgcggga aactggcgct
      841 cggtttcagc gcgggtagag agcgcgaaac tcgccgttca ttcgaatgga acaagagcaa
      901 agttctacag cttgcacacg gtctatcatt acacctggca aggtcaggac atggtggcca
      961 gccggtcact cccaacgtat ggaccatccg gaatgtcagg atcgggtgag ttttatcgtg
     1021 agaaactcaa ttatctcaat gtccagatgc aggccagtca gcccgtcgac atcctggtca
     1081 acccggacaa tccgaccgaa agtctcttga tcagggaagt ctgcccaggt atgtttttct
     1141 cgatcatctt cgggggaatt atgttcacgt ttggcttttt cggcctgctc gggctgctgt
     1201 ttccttctct tcgcctgctg tagatattct gaaggaattc gacagaattg atgactcagg
     1261 agaaacgcat gcgcgcgagt gaattcgaga gtatcgatca tgttccaccg gtagactgga
     1321 aagatgctcc tcaggaggtg cgcgacgcgc tcaacgccct gaaagaagga cgggatgcgc
     1381 ccgatgtctt cttcctgaaa agcgacctga cgcagacgca gaaagaaaga cggataacga
     1441 tcgcctgggc gtgtacgatc ttcgtggcag tctttggtct gggcgtctgt gcgccactcg
     1501 ccttgaaaca atctattcaa ggtggtct
//
LOCUS       DAORHD010001331         1528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_112292_length_1528_cov_2.564834, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001331 DAORHD010000000
VERSION     DAORHD010001331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112292_length_1528_cov_2.564834"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="PKO06_21355"
     CDS             complement(<1..842)
                     /locus_tag="PKO06_21355"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNV72269.1"
                     /translation="MTGAYGGELLYGLLSGMGATLGLGMIWRVCQNVFAHPADEQQSI
                     RAILLQAVRIPVYLCIVVFGLRVMLAEGPDFASRASWLVAADLFLSFFACIGVGEILF
                     GLGIDYYLRVSRGTEVPTIFKQLLKAIIYLIVGLSFLSTTYRIDITPLLTTSAIFSMV
                     IGLALQDVLSNFFAGLSVHISPPFKIGDWVKVGGFFGRVTESNWRATTLQLSSGESVT
                     LPNNDISKKDILNCSARLGCIMQEFNVGLGYDTSPETARRCLYAALEQAPRILKTPEP
                     KVYM"
     gene            1067..1438
                     /locus_tag="PKO06_21360"
     CDS             1067..1438
                     /locus_tag="PKO06_21360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72270.1"
                     /translation="MNKNIVITTVLASALTIGTAFAGNPGQSGQAGQAGQCDQGGCHK
                     PGFFKKMGESFKKGFKAGKKAGTKVYDSVQNKVMDSGTAVKKAVTGKKCKTFVKGHYN
                     NDGTHVKGHWRKLDCCSKKSQ"
BASE COUNT          411 a          416 c          401 g          300 t
ORIGIN      
        1 tccatgtaca ccttgggttc gggtgttttc agaattcgtg gagcctgttc caaagcggca
       61 tagaggcaac gccgagcggt ttcgggggac gtgtcgtacc caagcccgac gttgaattcc
      121 tgcataatgc acccgagtct cgcggaacag ttcaagatgt cctttttcga aatatcgttg
      181 ttcgggagag tcaccgattc tccacttgac aactgcaggg ttgtggctcg ccagttcgat
      241 tccgtgaccc gtccgaagaa ccccccgact ttgacccagt ctccaatctt gaacggaggg
      301 gaaatgtgta ctgacagacc ggcgaagaag ttgctcaaca catcctgtaa cgcgagaccg
      361 atcaccatac tgaagatcgc cgaggtcgtt aacaggggcg tgatgtcgat tctatacgtt
      421 gtcgacagga aggagaggcc gacaatcaga taaataatgg ctttcagcag ctgcttgaag
      481 atggtcggaa cttcggtccc gcggctgacc cgcaggtagt agtcgattcc gagtccgaag
      541 agtatttcgc cgactccgat acaggcaaag aaactcagaa agaggtcagc agcgaccagc
      601 caggaagcgc gactggcgaa gtcgggccct tcagccagca tgacacgcag cccgaatacg
      661 acaatgcaga gatacacggg aatccgaacg gcctgcagca gaatcgctcg aatggattgc
      721 tgctcatccg caggatgggc aaagacattc tggcagactc tccagatcat accgagtccg
      781 agagtcgccc ccattcctga caacagtccg tacagcagtt cgccaccata tgcgcctgtc
      841 atgaccgccg gctcccttca tcaatgcgct gcaaaaatcg tgcatttttg acgaaatttc
      901 agcgaggaaa atgccgaaaa atcatcatgg aaaactccag atcagaaaga atatcggttt
      961 gaagcgggtt ttcacgaagc agaatctggc atcgaagttg ctcaatgtct cgcatcacaa
     1021 cgcgagtcat aaaacacaag tttataagca tcaggaggaa ggttccatga ataagaacat
     1081 cgttatcacc accgtgttgg ccagtgccct caccatcggt accgctttcg ccggcaaccc
     1141 cggacagtca ggccaggctg gacaggcagg tcagtgcgat cagggcggat gccacaaacc
     1201 cgggttcttc aagaaaatgg gtgaatcttt caagaagggc ttcaaagccg gcaaaaaagc
     1261 gggcacgaaa gtctacgaca gcgtccagaa caaggtcatg gattccggca ccgcggtcaa
     1321 gaaagccgtc accggcaaga agtgcaagac cttcgtcaag ggtcactaca acaacgacgg
     1381 gacccacgtg aagggtcact ggcgcaagct cgattgctgc agcaagaaga gccagtaaca
     1441 ttcgatcgat cgaaacggcc ctcatcagag ggccgttttc ttttccgtct cattcagcca
     1501 ccctatctcc cgcatcagga gccaacag
//
LOCUS       DAORHD010001332         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_112369_length_1527_cov_3.477582, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001332 DAORHD010000000
VERSION     DAORHD010001332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112369_length_1527_cov_3.477582"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            563..>1527
                     /locus_tag="PKO06_21365"
     CDS             563..>1527
                     /locus_tag="PKO06_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72271.1"
                     /translation="MIKREMEAELKLLARQFPVVTVTGPRQSGKTTLCRKVFPKKTYV
                     SLENHDLREFAVQDPRGFLRSVSDGAILDEIQRAPQLTSYIQTLVDESPTPGRFILPG
                     SQQFEVSQTVSQSLAGRTALLKLLPFSQAELGARRIPDDLDHLLLTGFYPRIHDRGLE
                     PTKALADYFETYVERDLRQLIQLRDLHLFEKFVKLCAGRVGQLLNLNSLAADTGISHT
                     TARAWISLLEASYIVFLLPPWFRNVSRRLVKSPKLYFIDVGLAAMLLGIETTDQMSRD
                     PLRGHLFENFVIMEALKHRYNRGQRSNLSFFRDATGNEVDLILEQ"
BASE COUNT          331 a          455 c          429 g          312 t
ORIGIN      
        1 ttcgagttct atcagaagtg tggatacttc atcgtaggca ttgttcccaa cgccaacggc
       61 gagggaaaac ccgacatctg gatgtggaag aacctctcat ccgattcccc ttgtcctacg
      121 gatagatcag cagcctgcca aaccgcccga gatccgtaag tccctcgctc ctctagccgt
      181 ctcgctgatt cgccttgccg ctcaggctga cgagggcgag ttccgggcca ttcgccacct
      241 gtggcgaaac tgagcgttgg cggtcgagcc agattgatga tctcacaaac gcgaggtgac
      301 gaggtcgagg ttttccgacc cgggaccggc gagattgcgc cggccacatt caatacgtca
      361 ggtagccaga agcggcaacc gcgttccatg gatggccccc cgggaagaag ggtcagcgcc
      421 tcggcacaca aacaactcgc tgagaaaaac acgagtggtt cgcagagact ctgtcaagcg
      481 ctgaccaggc acctcgattc acattatttg catattgaaa gttgaacttg caatatgcaa
      541 ataacagaag tacctttgtg gtatgatcaa acgggagatg gaagcggagt tgaaactgct
      601 ggcgcggcag tttccggtgg tgacggtgac cggaccgcgg cagagcggga agacgacgtt
      661 gtgccgcaag gtgttcccga agaagacgta tgtcagcctc gagaaccatg acctgcgtga
      721 gtttgccgtt caggacccgc gggggtttct gcgatcggtt tcggatggcg caatcctcga
      781 tgagatccag cgcgcgcccc aactcacctc gtacattcag acactcgtgg atgagtcccc
      841 taccccgggc cggttcattc tgcccggcag tcagcagttc gaagtcagcc agacggtcag
      901 tcagtcgctg gcggggcgca cggcactgtt aaaactactg ccgttcagtc aagccgaact
      961 gggcgcgcgg cgcatcccgg acgacctcga tcatttgctg ctcacaggct tctatccgcg
     1021 catccacgac cgtggcctcg agccgaccaa agccttggcc gactacttcg aaacctatgt
     1081 cgaacgcgat ctgcgccagc tgattcaact gcgcgacctg cacctgttcg agaagttcgt
     1141 gaaactctgc gccgggcggg tcgggcaact gctcaacctg aacagcctcg ccgccgacac
     1201 cggcatctcg cacacgacgg ctcgcgcctg gatcagcctt cttgaggcga gttacattgt
     1261 ctttctgttg ccgccgtggt tcagaaatgt ttcgcgccga ctggtgaaat cgccgaagct
     1321 ctatttcatc gacgtcggtc tcgcggccat gcttctggga atcgaaacca ccgatcagat
     1381 gtcgagagac cctctgcgcg ggcacctgtt cgaaaacttc gtgatcatgg aagcgctcaa
     1441 acaccgctac aatcgcggcc aacgatcgaa tctttccttc tttcgcgacg ctacgggcaa
     1501 cgaagtcgat ctgattctgg agcagga
//
LOCUS       DAORHD010001333         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_112370_length_1527_cov_3.445652, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001333 DAORHD010000000
VERSION     DAORHD010001333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112370_length_1527_cov_3.445652"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="PKO06_21370"
     CDS             complement(<1..423)
                     /locus_tag="PKO06_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72272.1"
                     /translation="MSDSARFRLMIALFLIMIGSSAVFMYLQQSAIQAVIDDASDAAP
                     NEERAREEADKIEKQWQAATAAMKARDYRKAREHLGQAAPFDYQVNQLRETELRPSLP
                     FSTWWRQQTAAFSKATRSEFAQVARDALAKPAQSHQFVD"
     gene            579..1193
                     /locus_tag="PKO06_21375"
     CDS             579..1193
                     /locus_tag="PKO06_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72273.1"
                     /translation="MNQPQPLEVRLSRLCTAVLGAVGLLFFLVGLDLAYFRFLFDFTI
                     GEDKKLIFYAFLFFANICGGLIFVQMVWYFLLPPLALRVDEKGITFATGFRYTPCFIE
                     GKHVKSVGRGVTVDLTNKVDFLDVVVITFEPSPDIPSWKATPPGIQYFQHELSLSWFY
                     RDTSSFRIIDAVNGYLNAHIWSNKGLFFNAVASTPTKNTTSLPG"
     gene            complement(1182..>1527)
                     /locus_tag="PKO06_21380"
     CDS             complement(1182..>1527)
                     /locus_tag="PKO06_21380"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNV72274.1"
                     /translation="RELHPTRLITVNHLRGMVNLASAGVGVALVPAYCAAAEVRSGRL
                     RILFPKSQILEDWFSLYGRQGQRQLPKVSAFVAFMKSWKPTEFEAANEGQPSGAVRKE
                     AARRKPRENLSR"
BASE COUNT          302 a          468 c          415 g          342 t
ORIGIN      
        1 gtcgacgaac tggtgtgatt gtgccggttt ggcgagggca tcccgcgcga cctgggcaaa
       61 ctcgctcctg gtcgccttgg aaaaggcggc agtctgctgt ctccaccagg tcgagaaggg
      121 aagcgagggt ctcaactcgg tctcccgcag ctggtttacc tggtaatcga acggcgctgc
      181 ctgacccaga tgctcgcggg ccttgcggta atcgcgcgct ttcatggccg ccgtcgccgc
      241 ctgccactgc ttttcaatct tgtccgcctc ctcgcgcgcc cgttcctcat tgggcgcagc
      301 atcagaggca tcatcgatca ccgcctgtat ggctgactgc tgcagataca tgaacacggc
      361 cgaactgccg atcatgatca ggaacagagc aatcatcaac ctgaaacggg cagaatcact
      421 catgcatgcc acctctccga tgacatgata ccgcaaacgc gaatcggtgc tgaaacaaga
      481 ttctgaggtg catatcccgc ggccggtctg tataatctgc ctgaacgcgc acgaaccgtg
      541 atcgtcttcg agaccatcag tgcctggagg gcctgaccat gaaccaaccg cagcctctgg
      601 aagtacgtct gtcgcgactg tgcacggctg ttctaggagc cgtcgggttg ctgttctttc
      661 tcgtcggact cgatctggcg tatttccggt ttctcttcga cttcaccatc ggcgaagaca
      721 agaaactgat cttctacgca ttcctctttt tcgcgaacat ctgtggtggg ctgatcttcg
      781 ttcagatggt ctggtacttt cttctgcccc cgctggcttt gcgcgtcgac gaaaagggca
      841 tcacctttgc caccggcttt cgctacaccc cgtgcttcat tgaagggaag cacgtcaaat
      901 ccgtcgggcg cggagtcacg gtcgatctga cgaacaaggt cgactttctg gatgtggtcg
      961 tgatcacctt cgaaccgtct cctgacattc cctcgtggaa agccaccccg cctggcattc
     1021 agtatttcca gcatgaactg tccctgtcgt ggttttaccg cgacacctcg agtttcagga
     1081 tcatcgacgc ggtgaacggc tatctcaacg cccacatctg gagcaacaaa ggcctgttct
     1141 tcaacgcggt cgcgagcaca ccgacgaaaa atacgacctc gctaccggga tagattctcg
     1201 cgcggctttc gacgagcggc ttcttttcgc accgccccag acggctggcc ttcgtttgca
     1261 gcttcaaact cggtgggttt ccagcttttc atgaaggcga cgaaagcgct cactttgggg
     1321 agctggcgct gcccctgccg gccatagagt gagaaccagt cttccagaat ctgacttttc
     1381 gggaacagaa tgcgcaggcg accggaacga acttcggcgg cggcacagta agccggaacc
     1441 agcgcaacgc caaccccggc agatgccagg ttcaccatgc cccgcaggtg attgaccgtg
     1501 atcaggcgcg tcggatgcag ttccctt
//
LOCUS       DAORHD010001334         1527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_112420_length_1527_cov_2.230299, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001334 DAORHD010000000
VERSION     DAORHD010001334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112420_length_1527_cov_2.230299"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1071)
                     /locus_tag="PKO06_21385"
     CDS             complement(1..1071)
                     /locus_tag="PKO06_21385"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV72275.1"
                     /translation="MLGAIIGEICGSTGEARHNKDLRSGFCPPGSEITRPALETILLA
                     AACVNGDLEAEIEHLSSEGILKLTPLAWAFPTLEITIAKTELVLRLLKADAQTRKEGI
                     AFVTSLFLARTGRDKPSINAYLRDWLGVPMPVSATEVYTLVNSAVSGYTPFMRAFAFF
                     FQGNELGEILLRSLWYEDSGESVPGLATALAEAWYGGVPEYYSRTAWTLMPSVCCEGV
                     AQFHSIFKVPGPIHGMDSARRYLGATISCVNLDEHSDYEPPSYPRRTEAAYIGSLLAG
                     GVGDALGQHVEEQARRTFQEEPSLIKLDEFPPVRGFTPHASDDTQMTLFCAEGLLQAW
                     YRGTHRGICNPPVRRWAMRTTG"
     gene            1324..1497
                     /locus_tag="PKO06_21390"
     CDS             1324..1497
                     /locus_tag="PKO06_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72276.1"
                     /translation="MDNLDDIKKRLTIGLEVTPQETSSLAVKLSLLWDDKPFSDCVLN
                     LNFVQGQVSITVT"
BASE COUNT          321 a          464 c          418 g          324 t
ORIGIN      
        1 tcatccggtg gtgcgcatcg cccatcgcct gacgggcggg ttgcagattc cgcgatgcgt
       61 gccgcgatac catgcctgca acagaccttc cgcgcaaaac agcgtcatct gcgtatcatc
      121 ggaggcgtgc ggtgtgaacc cgcgcacggg cgggaattca tcaagtttga tcagcgaggg
      181 ttcttcctga aacgttcggc gggcctgttc ttcgacatgc tgtccgagcg cgtcacccac
      241 cccaccggcc agaagcgacc cgatgtatgc cgcttccgtt ctgcgcgggt aactcggagg
      301 ctcgtaatcc gaatgttcat cgaggttaac gcacgagatg gttgcgccga gatagcgtct
      361 ggcagaatcc attccatgga tcggccccgg caccttgaag atggaatgaa actgcgcaac
      421 accttcacaa caaacgctgg gcatcagtgt ccaggccgtt cgcgagtagt actccggcac
      481 gccaccatac caggcttcgg caagcgcagt ggccaggccg ggcacgctct caccgctgtc
      541 ctcataccac aacgaacgga gcagaatctc gccgagttca ttgccttgaa agaaaaaggc
      601 aaaggcacgc atgaatgggg tgtatccgga gacggcggaa ttcaccagcg tgtagacttc
      661 cgtggccgac accggcatgg gtacccccag ccagtctcgc agataggcat tgattgatgg
      721 cttgtcacga ccggtacgcg ccagaaacaa cgaagtgacg aaggcgatcc cttccttgcg
      781 tgtctgcgca tcggccttca gtagtcgcag aaccaactcg gtttttgcga tcgtgatttc
      841 cagcgtcgga aacgcccacg ccagcggcgt cagtttgaga attccctccg acgacagatg
      901 ctcgatctcg gcctccaggt cgccattcac gcaagccgcc gccaacagga tcgtttcgag
      961 ggcaggtctt gtgatctctg aaccgggcgg gcagaacccg gaccgcagat ctttgttgtg
     1021 tctggcctcc cccgtcgaac cgcagatctc cccgataatg gcaccaagca tacgcgtccc
     1081 tcccttcatc gtgtgcattc cggaaattcc ccggtgaccg cctgatacct ttactctggc
     1141 cgcgactctg gccgagtctg ccggcaagca cccagtgagg cttcgccatc ttcatccatt
     1201 ggctcggcgt tgagtgcggt atactttgtc tgtcttggca actcggacaa atgacaggct
     1261 ctgtgaaact gcgagctgtc acgtcggtct catgttctca agcgtcatca aaggagaaaa
     1321 tgtgtggaca atctcgatga catcaagaaa cggcttacca tcggcctgga ggttactcct
     1381 caggaaacca gctccctcgc cgtcaagttg tctttgctct gggacgacaa accgttttcc
     1441 gactgtgttc tgaacctgaa ctttgtccag ggccaggtct ctatcaccgt cacgtgagta
     1501 tgtttcaaga cgatcgatgg cccgaga
//
LOCUS       DAORHD010001335         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113084_length_1521_cov_3.342428, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001335 DAORHD010000000
VERSION     DAORHD010001335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113084_length_1521_cov_3.342428"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..578
                     /locus_tag="PKO06_21395"
     CDS             414..578
                     /locus_tag="PKO06_21395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72277.1"
                     /translation="MPRKSLDKMIRSYDDEDFSEVMIPENEVEYTVDIKKIRYFADGN
                     PEKKKNADPE"
     gene            575..739
                     /locus_tag="PKO06_21400"
     CDS             575..739
                     /locus_tag="PKO06_21400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72278.1"
                     /translation="MRYRFYINPETNQPHVLDHSVFEQEVIEAFENGFEDRHAVNKAR
                     SRIGQTYAGR"
     gene            841..1098
                     /locus_tag="PKO06_21405"
     CDS             841..1098
                     /locus_tag="PKO06_21405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72279.1"
                     /translation="MKRKRYIPAGTTEAEIKAIADHYDHQTPDEAVLEDERMLAIEVG
                     ARIIPLRPSLSQHLHELAREKKTTIDRLINSLLRKSMTKAA"
     gene            1241..1462
                     /locus_tag="PKO06_21410"
     CDS             1241..1462
                     /locus_tag="PKO06_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72280.1"
                     /translation="MTTKQLALKAIKTMPDEATLEEIMERLHFIGQVNAGIRDADEGR
                     TISHEEVKKRVRLWRIPPPRSTAASSAES"
BASE COUNT          414 a          398 c          422 g          287 t
ORIGIN      
        1 ggccccaaat cacaacgccg ccacgcaaca ggacatcgat tgcccgcaag tcatcggtca
       61 ggtgaacaag tggttgcagt gccgacgaag gtcgcacagt atcactgaag ctgtgcaagg
      121 accgcctacc ccccccaagt cggatgatca gtacagattg tttcgctcgc aaacgaatca
      181 cagagcaagc ttgagccagt ggcgtgagga cgaagcccct gcctttcagc gggcgtcttc
      241 ggtgccgaaa gcccgtcact cccaaaacca cacctcgaag gctggctccc ccttccgtga
      301 tggggcgcag gttgccaaag accttaccct gctgtaacct gaatttagac agtgatgttg
      361 tccggactgt attcacatga atccgggaag gtgatcagga aacaggaaac gatatgccgc
      421 gaaaaagcct tgataaaatg attcgctcat atgacgacga ggacttcagc gaagtgatga
      481 tcccggaaaa cgaggttgag tatacggttg atatcaaaaa gattcgctac ttcgcagatg
      541 gtaatccgga gaagaagaaa aacgcagatc cagaatgaga taccgattct acatcaaccc
      601 tgagacgaac caaccgcatg tgctggatca ctcggtgttc gagcaggaag tgatcgaagc
      661 ttttgagaac ggatttgaag atcgccacgc agtgaacaaa gcgcgatcgc gcatcggcca
      721 gacgtatgca ggaagataac tgagaatcat ctaccgtgtg tttcctggcg aactgttcat
      781 catcactgcg tatgatttga ctggaaaacc gctgaaggcg ttcagaagga ggcagaaata
      841 gtgaaaagaa aacgctacat tcccgccgga accaccgaag cagagatcaa agccatcgct
      901 gatcactacg accaccagac acccgatgag gcggtgctcg aagacgagcg catgttggcc
      961 atcgaggtcg gtgctcgcat catcccgttg cgcccgtctc tatcacagca tctgcatgaa
     1021 ttggcgcgag agaagaaaac caccattgac cgcctcatta attcgctcct gcgcaagagt
     1081 atgacgaaag ccgcgtagtc agtcagcttg gctaccgttg tctcgaaggc tggctccccc
     1141 ttccgtgatg ggggccgcag gttgccaaag atggtgacca gcggtaaagt aaacgaagaa
     1201 agcgattccg cgtatctgct tccatgaagg agccgacaaa gtgacgacga aacaactggc
     1261 gttgaaagcg atcaagacga tgccggatga ggcgacgctc gaagagatca tggagcgttt
     1321 gcatttcatc ggtcaggtga atgccggtat tcgagatgcc gacgagggcc gcaccatttc
     1381 tcatgaagaa gtgaagaagc gggttcgctt gtggcgtatt cccccaccgc gatcgacggc
     1441 cgcatcatcg gcggaatctt gatcaggcgc gggcgcagcg aacccaggaa gaagcagttc
     1501 acgtggccga acgtgggcga c
//
LOCUS       DAORHD010001336         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113100_length_1521_cov_2.950205, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001336 DAORHD010000000
VERSION     DAORHD010001336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113100_length_1521_cov_2.950205"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PKO06_21415"
     CDS             <1..1152
                     /locus_tag="PKO06_21415"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72281.1"
                     /translation="GFKTFVTSQNKLKLFLNAAKNKGNVRVLSSPHILAANHRKAIFK
                     IGEKLPLISSVRPSDEGPIKTFDVKEVGLELEVTPHINRSGQIDIEIRQTINAVDQYD
                     QKEGTARMTNREAQTNVVVTNGETLVLGGFIENKKDEKERKIPILSQVPILGKAFRSK
                     TKLHRKTELMVFLTPRIIDTIEDAQNVTELQKRRLSERKDVNRVLKQLQRTHVPLTAS
                     TSVIIDRYSDDWQYDFDRPDLDDLVWNSPATVKPDELTLSKLGRCPFGFGPSRRLKPA
                     LVRTWTKPSEGVVLRKTFQIEDPDVFRFLTLKVASDNAATVYLNGQKLDEDPMMKMKD
                     GHDFDYWNRVREKLSGKLLVEGVNTIVVLLGSDKDTDDGYFDMMLIGHR"
     gene            1162..>1521
                     /locus_tag="PKO06_21420"
     CDS             1162..>1521
                     /locus_tag="PKO06_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72282.1"
                     /translation="MRRFFPLLALPILVGATGLAEASAPSLSAQMPGMVIKEIKVSDK
                     NQEWSCHAIQSLSNPNDWYCTLSQPQLMTTQFEEKIRPDLTFYLFQKPDPRKPDRLIN
                     QGVLTCRIRTSPPSDILE"
BASE COUNT          391 a          430 c          386 g          314 t
ORIGIN      
        1 ggtttcaaga cgttcgtcac ctcgcagaac aaactgaaac tgtttctcaa tgccgcgaaa
       61 aacaaaggga acgtacgcgt tctgtcttcg ccgcatattc tcgccgccaa ccatcgcaag
      121 gccattttca agatcggcga gaaactgccg ctcatcagtt ccgtgagacc atctgatgaa
      181 ggtccgatca agactttcga cgtgaaggaa gtgggactcg agctcgaggt caccccgcac
      241 atcaatcgca gcgggcagat cgacatcgag attcgtcaga cgatcaacgc ggtcgatcag
      301 tacgatcaga aagaaggcac cgcccgcatg acgaaccgcg aagcgcagac caatgtcgtc
      361 gtcaccaacg gggaaaccct tgttctgggt ggattcatcg aaaacaagaa ggatgagaag
      421 gaacggaaga ttccgattct gtcccaggtg ccaatcctgg gaaaggcgtt ccgatcaaag
      481 accaagcttc accgtaaaac cgaactgatg gtatttctga ccccgcgcat cattgacacg
      541 atcgaagacg cccagaatgt caccgaactg cagaaacggc gtctcagcga aagaaaggat
      601 gtgaaccgcg tcctgaagca acttcagcgc actcacgttc ctctgacggc atcgacctcg
      661 gtcattatcg accggtattc ggacgactgg cagtatgatt ttgatcgccc cgacctggac
      721 gatctggtct ggaattctcc ggcgacggtc aagccggatg aactcacctt gtcgaaactc
      781 ggtcgctgtc catttggctt tggtccctct cgccgtctga aaccggctct ggttcgcacc
      841 tggacaaaac cctccgaagg agtcgttctg cgcaagacct tccagatcga agatccagat
      901 gtttttcgct ttctgactct gaaagtcgcc agcgataacg cggcgacagt ctatctgaac
      961 ggccagaagc tcgatgaaga cccgatgatg aaaatgaagg acggacatga tttcgactac
     1021 tggaatcggg tacgggaaaa gctgtcggga aaactgctgg tggaaggagt caacacgatt
     1081 gtcgtacttc tcggcagcga caaggataca gatgatggct actttgacat gatgctcatc
     1141 ggtcatcggt aagcggaatc catgcgtcgg ttcttcccgc ttctcgcgct accgattctc
     1201 gtcggagcca caggactggc cgaggcaagt gcgccttctc tgtcagcgca aatgccgggc
     1261 atggtgatca aggaaatcaa agtgtccgac aagaatcagg agtggtcatg ccacgctata
     1321 cagagcctct ccaatccgaa cgactggtac tgtactctca gccagccaca actcatgacc
     1381 acccagttcg aggagaaaat ccgacccgat ctcacgttct atcttttcca gaaacctgat
     1441 ccccgcaagc ctgaccgact catcaatcag ggagtgctga cctgtcggat cagaacctcc
     1501 cctccttccg acattctcga g
//
LOCUS       DAORHD010001337         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113466_length_1518_cov_3.382092, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001337 DAORHD010000000
VERSION     DAORHD010001337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113466_length_1518_cov_3.382092"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKO06_21425"
     CDS             <1..501
                     /locus_tag="PKO06_21425"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HNV72283.1"
                     /translation="PHVFQSGEDLTPEAVIQRWNDLKVVEKREKARLSHRMEEASGFG
                     SPVQAALQIQKEAARVGFDFPNRREVVSKLVEESREMLAACEAKSRDDLDHELGDMLF
                     SLVNVARLHEIDLEQSLRNTNAKFIRRFRLVEDEVEKRGGFNGKSLTELDDIWNVVKA
                     QEKTLK"
     gene            complement(516..>1518)
                     /locus_tag="PKO06_21430"
     CDS             complement(516..>1518)
                     /locus_tag="PKO06_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72284.1"
                     /translation="PDTPPASSTPELSPSVKPAPPAARPATRTPPPTSGTLAKPPVAP
                     APPPLTSGESSRLPSAAQPTTEEVVRATEPRETSPSAPAAPEPPKRLAWLDAIGCPQE
                     NFAAWFQGLSQRERVDALTRMEGLATDASLVQSLGIILANETDTFVLSKTVKVYGKLA
                     GAGSLARLELFLDHADARVISNALEALGISGGPKELQKLTQFLDHPDSRIRGTAAKHL
                     WSSHPDKAMAVVERMRRSAIVWEREAARHALKSCPHPQGQELLKQLETKPAQTTSQKV
                     AVAEPLPVWRVWMDKPVEIGLDKPLRLWEFLIFVSGLIAFGNFIALPLVDWLFPLTN"
BASE COUNT          316 a          381 c          504 g          317 t
ORIGIN      
        1 ccgcatgtct ttcagagcgg cgaagatctg acacccgagg cggtgattca acgctggaac
       61 gatctgaagg tggttgaaaa acgagaaaaa gccaggctgt cacacaggat ggaagaggct
      121 tccggattcg gatcaccggt acaagccgct ctgcagattc agaaagaggc ggcgcgcgtg
      181 ggtttcgact ttccgaaccg gcgcgaggtc gtgtcgaagc ttgtcgaaga gagccgtgag
      241 atgcttgccg cctgtgaggc gaagagtcgc gacgatctcg atcacgagct cggagacatg
      301 ctgttttccc tggtcaatgt cgcacgtttg catgagatcg accttgaaca gtcgctgcgc
      361 aacacgaatg ccaagttcat tcgccggttt cgcctcgtgg aagatgaggt cgaaaaacga
      421 ggtggattca acgggaagtc gttaacggaa ctggacgata tctggaacgt ggtcaaggct
      481 caggaaaaaa cgctgaagta gcatgttccc gatcttcagt tggtgagggg aaagagccag
      541 tcgacaagtg gcagggcaat gaagttgccg aaagcgatca ggccgctgac gaagatcagg
      601 aactcccaca gtcgcaacgg tttgtccagg ccgatttcaa cgggtttgtc catccagact
      661 cgccagacgg gaagaggctc tgcaaccgcc actttctgag acgtggtctg cgctggtttt
      721 gtctcgagct gtttcagaag ttcctggccc tgtggatgtg gacagctttt cagggcgtgt
      781 cgagccgctt ctctttccca gacgatggcg gaacgcctca tgcgttcaac gacggccatc
      841 gctttgtccg ggtgcgagct ccagagatgt ttggccgcgg taccgcggat gcgagagtcc
      901 gggtgatcga gaaactgtgt cagcttctga agctctttgg ggccaccaga aataccgagt
      961 gcttcgagtg cgttagaaat gacgcgagca tctgcatggt cgagaaacag ttcgagtcgc
     1021 gcgagactcc cggcgccagc aagttttcca tagaccttca cggtcttcga gagcacgaag
     1081 gtatcggttt cgttcgcgag tattatcccc aatgattgaa cgagactcgc atcggtggcg
     1141 agtccctcca tgcgagtgag ggcgtcgacg cgctcgcgtt gactcaagcc ctgaaaccag
     1201 gccgcaaagt tttcctgagg acagccgatc gcgtcaagcc acgccagtcg tttcggcggc
     1261 tcgggcgccg caggggctga aggtgaggtc tctcgaggct cggtggctcg gacaacctct
     1321 tcggttgtcg gttgagcagc cgaaggaagt cttgaggatt ctccggaggt gagcggtggg
     1381 ggcgccggtg ccaccggggg tttggcgagt gttccgctcg tcgggggggg agttcgtgtg
     1441 gcaggtctgg cggcgggtgg cgcgggtttg acagaaggcg aaagttcagg cgtggaactc
     1501 gctggcgggg tatctggc
//
LOCUS       DAORHD010001338         1518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113484_length_1518_cov_2.888585, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001338 DAORHD010000000
VERSION     DAORHD010001338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113484_length_1518_cov_2.888585"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PKO06_21435"
     CDS             <1..427
                     /locus_tag="PKO06_21435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72285.1"
                     /translation="GGSEGGATPPAGAVTGELSTVPYRPEYGQATIDTSSDERAIVSA
                     ATWVKEKHPDLFPEGDDRDKAYVMMTHVIGILRAKGYDVHRVVNHPSRPVGHALRYGS
                     DALVLKGKIYDCYGALGDPNRSTPQAMYVGPYAAGRLKE"
     gene            complement(512..724)
                     /locus_tag="PKO06_21440"
     CDS             complement(512..724)
                     /locus_tag="PKO06_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV72286.1"
                     /translation="MKISARNVLKGKVKKIEAGAVNTEITLELPGGQEIVSIITKHAA
                     DSLGLKVGKDAYAIIKASSVMMGADH"
     gene            complement(781..1263)
                     /gene="tsaA"
                     /locus_tag="PKO06_21445"
     CDS             complement(781..1263)
                     /gene="tsaA"
                     /locus_tag="PKO06_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNV72287.1"
                     /translation="MDGICLRPIGIIRTPHSQVSGMPIQPCGADRVTGTIEIHAEFVA
                     GLADLEGFSHVWLIYHLHQNQRPCLRVKPFLDDAEHGIFATRSPSRPNPVGLSLVTLV
                     AVNGNRLMVENIDVLDGTPLLDIKPFVPAFDSVSDPRIGWYEGKLEKAVTTRSDRRFV
                     "
BASE COUNT          351 a          432 c          405 g          330 t
ORIGIN      
        1 tggtggatca gaaggcggag caaccccgcc ggccggcgca gtcacaggag aattgtcgac
       61 cgttccctat cgcccggagt atgggcaagc gaccattgac accagcagcg acgagcgcgc
      121 catcgtcagt gccgcaacct gggtgaagga aaagcacccg gatctgtttc cggaaggcga
      181 cgaccgcgac aaagcctacg tcatgatgac acacgtcatc ggcattctca gagccaaagg
      241 ctacgatgtt caccgcgtgg tcaatcaccc ctcgcgtccc gtcggtcacg ctctgcgtta
      301 cggctctgac gcactggtac tcaaaggaaa gatctacgat tgctacggtg cgttgggcga
      361 cccgaaccgc tctactcccc aggcgatgta tgtcggcccc tatgccgcag ggcgtttgaa
      421 agagtagtta tcgacagccc gagagtacaa cgtcgattct ttcgggctgt ctcagagaga
      481 ctgctccaga ggtcgtgaaa cgttcagtcg ctcagtggtc ggctcccatc atgacactcg
      541 aagctttgat gatggcatag gcgtccttgc cgactttcag accgagcgaa tccgctgcgt
      601 gcttggtgat gatcgagaca atttcctgac cacccggcag ctcgagcgta atctcggtgt
      661 tcaccgcgcc cgcctcgatc ttcttcactt ttcccttgag aacgtttctg gcactgattt
      721 tcatacaact ctccttcgtg ttgatccggg gaaaacccgg ggaactgaca gctcacgctg
      781 tcacacaaac cgacgatcag agcgcgtcgt caccgccttt tccagtttgc cttcatacca
      841 gccgatgcgt ggatcggaga cggagtcgaa tgcaggcacg aacggcttga tatcgagcag
      901 aggggttcca tcgagcacat cgatgttttc aaccatgagg cgattgccat tcacggccac
      961 cagcgtcacc agcgacaacc cgacaggatt cggcctggac ggagaacgcg tggcgaagat
     1021 cccgtgttcg gcatcatcga gaaacggttt cacgcgcagg catggccgtt gattctgatg
     1081 cagatggtag atcagccaca catgagaaaa gccttccagg tctgccaatc cagcgacgaa
     1141 ctcggcgtga atttcaatag taccagttac tcgatctgct ccacaaggct gtataggcat
     1201 tccagatact tgagaatgcg gtgtgcgaat gataccaatc ggtcgcaggc agattccatc
     1261 cacagtgctc tccacgtctt tcgctctctc acatgacagt ttgacttata gtagcgcaga
     1321 tggggagtgt tgaaaagccc tgcatgtgtt ctttccccat gagtccccgg atgccgcacg
     1381 aaaaactcct tttcttcgcc aaaaaaaagg tctacacttg atagcattca gtatcattcg
     1441 gaggcgaaca tgtgtgatcc ccagaaactg ttgacgatca aagatgcggc ggatctgctg
     1501 agcgtgaacc aggaggtt
//
LOCUS       DAORHD010001339         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113607_length_1517_cov_2.729822, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001339 DAORHD010000000
VERSION     DAORHD010001339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113607_length_1517_cov_2.729822"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..717)
                     /locus_tag="PKO06_21450"
     CDS             complement(55..717)
                     /locus_tag="PKO06_21450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72288.1"
                     /translation="MSVIDEQIVAKTFPRAMIREYRAIWRTLVLMRCRLGRLFVKRVQ
                     ARILTLGLWACLLGAMAHAQLNATEPAVPPVPVRVVAVADVEDLRHGRYAVREFLDVY
                     NDDYARTLEVTRVTADTWVGQKRDLEQENWKSDPESIFVPPATVARVAERQRIVAGLP
                     LRNWWVRWLRFTVVTDRGTFQSNFIASPLKPPPRIESIITQPQLDSYRSPSLPVPPPR
                     EP"
     gene            complement(799..>1517)
                     /locus_tag="PKO06_21455"
     CDS             complement(799..>1517)
                     /locus_tag="PKO06_21455"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNV72289.1"
                     /translation="ILFSNDAFGQHYASSQRFADECDPCELWQEAAKYYANILTPYSA
                     RVTAKVKEFAALNWPIEMICPSHGVLWRKDPMQIVNQYLDWASGKADNSVVVAFDTIW
                     HGTEKMARAICRGLEAEGVVYRLYNVGGSDFNDIMTEILKSRGVLIGGPTLNNNLMPT
                     LTPVLESIRGLRFINKIGAAFGTYGWSGEGVKRIEEFLEAGGIKIVQVGYRVNYAPTD
                     EDLAQCEQFGRQFAKQVKAL"
BASE COUNT          332 a          479 c          410 g          296 t
ORIGIN      
        1 acgcgctcgt gtacctcgag cgcacgacgc cgtcgcgacc gttccgcgcg acgctcaggg
       61 ctctcgcgga gggggtaccg ggagactcgg tgaccggtac gagtcaagct gtggttgcgt
      121 gatgatcgac tcgatgcgcg gtggcggttt cagcggcgac gcaatgaagt ttgactgaaa
      181 ggtgccgcga tcggtgacca ccgtgaacct caaccagcgc acccaccagt tccgcagcgg
      241 caaacccgcg acaatccgct gtcgttcagc gacacgcgcc accgtggccg ggggaacgaa
      301 aatcgactcc gggtcactct tccaattttc ctgttcaaga tcacgcttct gaccaaccca
      361 ggtatctgcc gtcacacgtg tgacttcaag cgtccgggcg taatcgtcat tgtagacatc
      421 aagaaactcc cgcaccgcgt atcgtccgtg tctcaggtcc tcgacatcgg caaccgcaac
      481 cacccgaact ggtactggcg ggacagccgg ctcagtggca ttcagctggg catgagccat
      541 cgcccccaac aggcaagccc acagccccag ggtcagaatt cgtgcctgca cgcgcttcac
      601 gaaaagccga ccgagacgac agcgcatcag caccaacgtt cgccagatgg cacggtattc
      661 tctgatcatg gcccgcggaa aagttttcgc aacgatctgt tcatcaatga cagacaccct
      721 cgacatcctg aaatggtctg cctgtacagg aaagaggtgc agggaaccgt accggcttcc
      781 tgcacctcac atctacaatc agagcgcttt tacctgctta gcaaactgac gaccgaactg
      841 ctcacactgc gcgagatctt cgtcggttgg ggcgtagttc acgcggtaac ccacctggac
      901 aatcttgatt ccgccggctt caaggaattc ctcgatgcgc ttgacgcctt ctccgctcca
      961 gccatacgtg ccaaacgcgg cgccgatttt gttgatgaaa cgcaacccgc gaatgctctc
     1021 gagaaccgga gtcagggtcg gcatcagatt gttgttcagc gtcggaccgc cgatgagcac
     1081 cccccgcgat ttgagaatct cggtcatgat gtcgttgaaa tcggagccac cgacattata
     1141 aagacgatag accacgcctt cagcttccag accacggcag atggctcgcg ccatcttctc
     1201 ggtaccgtgc cagatcgtgt cgaaggccac aaccaccgag ttgtccgcct tccccgaagc
     1261 ccagtcgaga tactggttca cgatctgcat ggggtcctta cgccacagca cgccgtggct
     1321 ggggcagatc atctcgatcg gccagttcag cgcggcaaac tccttgacct tggcagtgac
     1381 gcgggctgaa tacggagtca gaatgttcgc ataatacttc gccgcttcct gccacaattc
     1441 gcaggggtcg cactcgtcgg cgaaacgctg tgaagaagca taatgctgcc cgaacgcgtc
     1501 gttcgagaac agtatct
//
LOCUS       DAORHD010001340         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113846_length_1515_cov_3.698630, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001340 DAORHD010000000
VERSION     DAORHD010001340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113846_length_1515_cov_3.698630"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..603)
                     /locus_tag="PKO06_21460"
     CDS             complement(10..603)
                     /locus_tag="PKO06_21460"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72290.1"
                     /translation="MPRSYQARRGVTLLELVIAAAIMTIMAGALYGVLSHFSYTRSIA
                     NARGTAKQEVELALRQIERDIAGARAGTFDDSTSNKIAMTTTQKIDTVAGESGFEELT
                     VEYTWGSDGALKRKETKSGTSREKVLMQNLKTLEVGREAASGSVHLKLVAEVKPEGWP
                     TALQIHSQDVLATIREEAAGAGSDTRWVRPRDLKNQF"
     gene            complement(617..1054)
                     /locus_tag="PKO06_21465"
     CDS             complement(617..1054)
                     /locus_tag="PKO06_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72291.1"
                     /translation="MRNSKFGVSLLEILVAGFILCLGIIPLYRALSGQSAAEIETTKF
                     AMAKEILTSVRQEILAHTFAEMKNLVPTGTGGGILTGQPYPIALTKVLNAQTKFKDFE
                     LEVRGTFTDSTQTMIQYTAEIKFTSQGGKIQKEVLPFMQISPN"
     gene            complement(1041..>1515)
                     /locus_tag="PKO06_21470"
     CDS             complement(1041..>1515)
                     /locus_tag="PKO06_21470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72292.1"
                     /translation="PNRTSGGAFDCTGVTFVKTGEVEISFPKVKGKGLIVANWHMAVK
                     SDIEREGALGETSLGLIARLGRMTFHAPVKHVEAACFSNFTPINQTSGVLKIDGNMIV
                     NHFDRREFNNIHVFYNGPGCRTSLLSVARDVGKYEPKRYHVALGNQWSRFEYEKQ"
BASE COUNT          301 a          415 c          440 g          359 t
ORIGIN      
        1 ccgcgagcgt tagaactggt tcttgagatc gcgtgggcgc acccagcggg tatcgctgcc
       61 ggcgccggcg gcttcttcac gaatggtggc gagtacgtcc tgcgagtgaa tctgcagggc
      121 ggtgggccac ccttcgggtt tgacctcggc caccagcttc aggtgcactg aacccgaagc
      181 cgcttcgcgc ccgacctcga gagtcttcag attctgcatc agaaccttct ctcgagaggt
      241 gccgctcttg gtttctttgc gtttcagcgc gccatctgac ccccaggtgt attcgacggt
      301 cagttcctcg aatccgctct cgcctgcaac cgtatcgatt ttctgcgtgg tggtcatggc
      361 aatcttgttt gaggttgagt cgtcaaatgt cccagctcga gctccggcaa tgtcacgttc
      421 aatctgcctg agagcgagtt cgacttcctg cttggctgtg ccgcgtgcat tggcgatcga
      481 gcgggtataa ctgaagtggc tgagcacacc gtacagtgct cccgccatga tggtcatgat
      541 cgcagcagcg atgaccagtt cgagaagagt gacgcctctg cgcgcctggt aagaacgagg
      601 caagcaacaa gacatatcag ttcggtgaaa tctgcatgaa tggcagcact tctttctgaa
      661 tcttgccgcc ctggctggta aacttgattt cggcggtgta ctgaatcatg gtctgggtcg
      721 agtcggtgaa tgtgcctcgc acttcgagct caaagtcttt gaacttggtt tgtgcattca
      781 gcacttttgt cagagcgatg gggtagggtt gcccggtgag aatgccgccg cccgtgcctg
      841 tgggcaccag gtttttcatc tcggcaaacg tgtgcgcgag aatctcctgc cggaccgagg
      901 tgagaatctc tttggccatg gcaaacttgg tggtttcaat ctctgccgcg ctttgccccg
      961 agagggcgcg gtacagggga atgataccca gacacagaat aaagccggca acgagaatct
     1021 cgagcagcga gacaccgaac ttactgtttc tcatattcga atcgtgacca ttggtttcca
     1081 agggccacat gatacctctt cggttcgtat ttgccaacat ctcgcgccac cgaaagtaaa
     1141 gaagtgcggc agccggggcc attgtagaac acgtgaatgt tgttgaactc gcgccgatcg
     1201 aaatgattga cgatcatgtt gccgtcgatc ttgagcactc cgctggtctg attgatcggc
     1261 gtgaagttcg agaagcaggc agcttcgaca tgtttgaccg gcgcatgaaa ggtcatgcgt
     1321 ccgagtcggg cgatcaggcc gagcgaggtt tcgcccagtg cgccctcacg ctcgatatca
     1381 gacttgacgg ccatgtgcca gttcgcgacg atcaaaccct tgcccttgac cttggggaac
     1441 gagatctcaa cctcgccggt cttcacgaac gtgacgccgg tgcagtcaaa tgcgccgcct
     1501 gaggttcgat tcgga
//
LOCUS       DAORHD010001341         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_113851_length_1515_cov_3.486986, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001341 DAORHD010000000
VERSION     DAORHD010001341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113851_length_1515_cov_3.486986"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKO06_21475"
     CDS             <1..404
                     /locus_tag="PKO06_21475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72293.1"
                     /translation="DKFIGDSVVAIFYDRPGESGVIQAIRAALEMNRQHRLIQQARML
                     QGDFSYRMGVGIDCGALMVGTIRSQGRHEHSIIGDARHNADVLESLSRYGQHTRIVLS
                     ADACQAFPDGEYLALSQGPGFELIGLKESAA"
     gene            401..>1515
                     /locus_tag="PKO06_21480"
     CDS             401..>1515
                     /locus_tag="PKO06_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72294.1"
                     /translation="MKTRLTLISALLFLMMPLILALSERKWAEKLEEERNIDEIHSRA
                     TAQLRQMSESTNQTVQLQNLLNVFFHTIVRSYLRTATLNPEWVKAWYRRMLWFILPDH
                     DLVILETSASGTRVIFDNGGPLGFSGQTGKRFLPLMSRGMSIATISFPLDELWRALHF
                     PLLLRDQKDYYHGRIQYFHGIEGARGFFWKSAAHPDARRQLILGITIDTQKLDPLFDL
                     KAMVKGHRSKEAGIGFINLAQGRNFLSSFFSAYPPLRQRLLRARPGAKGFPRFERHGP
                     LLLFSLPPSPGKSHQPLVAFRLPHARGSTRTTTLQFLILLSILVVIGVGIVTERLLFH
                     RGLQLRVGRILLAAFLLSAFIPIIGATVITRQNLLET"
BASE COUNT          295 a          463 c          411 g          346 t
ORIGIN      
        1 tcgacaagtt catcggcgac tctgtcgtcg ccatcttcta cgatcgtccg ggtgagagcg
       61 gagtcataca ggccattaga gcggctctcg agatgaatcg ccagcaccgt ctcattcagc
      121 aggcccgaat gctgcaggga gatttttcgt atcgcatggg ggttggcatc gattgcggcg
      181 ccctcatggt cggcaccatt cgttcgcagg gacggcatga gcactccatc atcggtgacg
      241 cccgccacaa tgctgatgtc ctcgaaagcc tctcgcgcta tggccaacac acgcgcatcg
      301 tcctttcggc ggacgcctgt caggcattcc cggacgggga gtatctcgcg ctttcccagg
      361 ggccaggctt cgagttgatc gggctcaagg agtccgccgc atgaaaaccc gcctgaccct
      421 catcagcgcg ctccttttcc tgatgatgcc tctgatcctg gctttgagtg agcgcaaatg
      481 ggccgaaaaa ctggaagaag aacgcaatat cgatgaaatt cattcacggg cgaccgcgca
      541 gttgcggcag atgagcgagt cgacgaacca gaccgttcag ttgcagaacc tgctcaacgt
      601 ctttttccac accattgttc ggtcgtatct gcgcaccgcg acactgaacc ccgagtgggt
      661 caaagcctgg tatcgacgaa tgctctggtt cattctccct gaccacgacc tggtgattct
      721 ggaaacgtcg gcgagtggta cccgggtgat cttcgacaac ggtggaccgc tgggtttctc
      781 aggacagacg ggcaaacggt ttctgccctt gatgagccgc gggatgtcaa tcgcaacgat
      841 ctcctttcct ctggatgaac tctggcgagc ccttcatttc ccgttactgc tccgtgacca
      901 gaaagactac tatcatggtc gcattcaata ctttcacggg atcgaaggag cgcgcggatt
      961 cttctggaaa tcggccgctc acccggacgc acgacgacaa ctcatcttgg gtatcacaat
     1021 cgacactcag aaactcgatc ccctgttcga cctgaaggcc atggtcaagg gacatcgctc
     1081 gaaggaggca ggcattggtt tcatcaatct ggcgcagggg cgaaactttc tgtcgtcttt
     1141 cttcagtgcg tacccaccgc tgcgccagcg actgttgcgg gcaagacctg gtgccaaagg
     1201 gttcccccgc ttcgagcgac acggacccct gctcctgttt tcgcttccgc cgtctcccgg
     1261 aaagtcgcat caaccactcg tcgcttttcg cctcccacac gcgcgtggct cgacgcgtac
     1321 gactactctc cagtttctca ttctgctgag catactcgtc gtcattggtg tgggtatcgt
     1381 cacggagcgt ctgctgtttc atcgcggact tcagctgcgg gttggccgca tcctgctcgc
     1441 ggcgtttctg ttgtcggcct tcattcccat catcggggca acggtcatca cccgacagaa
     1501 cctgctggaa acgga
//
LOCUS       DAORHD010001342         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_114070_length_1513_cov_3.464335, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001342 DAORHD010000000
VERSION     DAORHD010001342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114070_length_1513_cov_3.464335"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1105
                     /gene="cas1"
                     /locus_tag="PKO06_21485"
     CDS             101..1105
                     /gene="cas1"
                     /locus_tag="PKO06_21485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNV72295.1"
                     /translation="MQDAVLYVSQPGARLTRTGERLIVRAIDGKIVSDVPMFRLDQVI
                     CFGTVEPSGSALLALSRRGIDLVFLSQDGRFKARIAPSRPRAVQCRVKQYQRSLDPEF
                     RLTMARTLVGGKLNLSRVWLLRQNRNRDSRLTSQILGISGCIDSLREACSVDQLMGIE
                     GTAARYHFEALKLILKQDLGFNGRVRRPPKDPVNALLSFGYTLLFNRVVSAVEQVGLD
                     PMISNLHGLEDRRPSLALDLMEEFRTLIVDSVVVGLINRMEICATDFDDLGEKGVQMK
                     ERTLARFVKAFQDRLNDTMPHPREDRSFTLRDLIVQQAYQYKSVILGEASEYQPMTTR
                     "
     gene            1102..1479
                     /gene="cas2"
                     /locus_tag="PKO06_21490"
     CDS             1102..1479
                     /gene="cas2"
                     /locus_tag="PKO06_21490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNV72296.1"
                     /translation="MRGQFYIICYDTPSNKRRRKLHAYLKNYAVAVQKSVFETFLDGP
                     TFDRFLSKLEKFIDPAVDSVRIYGMTREAQKEMKVYGFPGLLEDPDHFFIGKLDNPVP
                     LTTEVTAEKLAEEEDLDDVADAL"
BASE COUNT          319 a          446 c          424 g          324 t
ORIGIN      
        1 caccatcacc acggcgatcg gagagtaccg ccgcctgcct tctctgccga gccccgatcg
       61 agggttcgac gagacagaat cctaaacatc ggggaacatc atgcaggatg ccgtactgta
      121 tgtttcgcaa ccgggcgcac gcctgacacg caccggagag cggctgatcg ttcgagcgat
      181 cgacggaaaa atcgtttcgg atgtccccat gttcaggctc gaccaggtca tctgcttcgg
      241 aaccgtcgaa cccagcggtt ccgcgcttct cgcgctttct cgtcgcggca tcgacctcgt
      301 tttcctcagt caggacgggc gtttcaaggc tcgcatcgcc ccttcccgcc cacgtgccgt
      361 tcaatgccgg gtcaagcagt atcagcgttc gctcgatccg gagtttcgcc tgaccatggc
      421 gcgaacgctg gtcggaggca aactgaacct cagccgcgtc tggcttctgc ggcagaatcg
      481 caatcgcgac agccgcctga cttcgcagat tctcggcatc tccggctgta tcgattccct
      541 gcgagaggct tgttccgtcg atcagctgat gggcatcgag ggaacggccg cgcggtacca
      601 ctttgaggca ctcaagctca ttctcaaaca ggatctcggc ttcaacggac gcgtgcgccg
      661 cccgcccaag gacccggtga acgccctgtt gagtttcggc tacacgcttc tctttaaccg
      721 cgtcgtatcg gcggtcgagc aggtcgggct cgatccgatg atctcgaatc tgcacgggct
      781 tgaagaccgc cgcccctctc tcgcgctcga tctgatggag gagttccgca ccctgatcgt
      841 cgactcggtc gtcgtcggcc tgatcaaccg catggagatc tgcgcgaccg atttcgacga
      901 cctgggcgag aagggggtcc aaatgaagga acggacactg gcgaggtttg tgaaggcgtt
      961 tcaagaccgg ttgaacgaca cgatgccgca tccgcgcgaa gatcgctcat ttacgcttcg
     1021 cgacctgatt gttcagcagg cctaccagta caaatccgtc attcttggag aggcatcgga
     1081 gtatcagccg atgacgacgc gatgagaggc cagttttaca ttatctgcta cgatacgcct
     1141 tccaacaaac ggcggcggaa actgcacgcg tacttgaaga attacgccgt agcggttcaa
     1201 aagagtgtct tcgaaacctt tctcgacggt cccacgttcg atcggtttct ttcgaagctc
     1261 gagaagttca tcgatccggc cgtcgatagc gtccgcatct acggaatgac gcgagaggcc
     1321 cagaaagaga tgaaagtcta cgggttcccg gggttgttgg aagaccccga tcactttttc
     1381 attggaaaac tcgacaatcc cgtgccgtta acaacagaag ttactgctga aaaactggca
     1441 gaggaagagg atctcgacga cgtggccgac gccttgtagc ccgaatagta tttatggtat
     1501 actatttgtg gta
//
LOCUS       DAORHD010001343         1513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_114104_length_1513_cov_2.834705, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001343 DAORHD010000000
VERSION     DAORHD010001343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114104_length_1513_cov_2.834705"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKO06_21495"
     CDS             <1..318
                     /locus_tag="PKO06_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72297.1"
                     /translation="EREFNQAREGMGQFTVRTDVGLHFGPVISGKVGSRCGMIDFTVI
                     GDTVNTAARIQAQAARDHAPAIFLSRNVLDNLEPKPRLETLPDITLKGKSTRLSLFRL
                     LDA"
     gene            complement(383..1477)
                     /locus_tag="PKO06_21500"
     CDS             complement(383..1477)
                     /locus_tag="PKO06_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HNV72298.1"
                     /translation="MGGFPPATIDWQKLIPLIGPASAAVARYDGSLSAIPNVSVLLSP
                     LTTQEAVLSSRIEGIQATMGEVLEYEAEGDREFSADRQADILEVLNYRSAMHIAETAL
                     EKLPLCMRVIRDAHRELLKGVRGQGNSPGELRTGPNWIGPKGCTIEQARFVPISADKL
                     PAALSSWEKYLHSDTPDKLVQLALLHAEFEALHPFLDGNGRLGRMLIPLFLWQTGLIQ
                     KPVFYISAFFEQNREEYYERLLTVSRDGNWSDWCLFFLKAVQTQAEENQKKAKAILNL
                     YESLKLRIAAWTHSQYAIHAQDWIFARPIFRSSDFVASAKIPAPTARRVLAILVENNV
                     LKRVVEGRGRRGAVFSFPELLNIAEGRAVF"
BASE COUNT          361 a          414 c          402 g          336 t
ORIGIN      
        1 gagcgtgagt tcaatcaagc gcgggaaggc atgggccagt tcaccgtgcg taccgacgtg
       61 gggctccact tcggccctgt gatttcggga aaggtgggct ctcgatgcgg aatgatcgat
      121 ttcaccgtga tcggtgacac cgtgaacacc gccgcccgca tccaggctca ggccgcccgt
      181 gatcacgctc ccgccatttt cctgagccgg aacgtcctgg ataacctcga gccaaagccg
      241 cggctggaga ctctcccgga catcaccctc aaaggaaaat ccactcgcct gtccctgttc
      301 cgcctgctcg acgcctgatt ccttgccgat cataaaacgc gaaaaatgag cgacaaatga
      361 gtttgtcgct caccgatgat cgtcaaaaca cagctctccc ttcggcgatg ttcaaaagct
      421 caggaaacga aaacaccgct cctcttcgcc cacgcccctc gaccaccctt ttcagcacgt
      481 tattttcgac gagaatggcg aggacacgac gagccgttgg ggcagggatc ttggcagacg
      541 cgacaaaatc agaactccga aaaatgggcc gggcgaaaat ccagtcttgt gcatgaatcg
      601 cgtattgtga atgggtccag gccgcgatcc gtagcttcag agactcatac agattgagaa
      661 tggcttttgc ctttttctga ttctcttcgg cctgggtttg aaccgccttc aaaaagaaaa
      721 ggcaccagtc gctccagttg ccatctcgcg atacagtcag gagacgctcg taatactctt
      781 cgcgattctg ctcgaaaaat gcgctgatgt agaaaaccgg tttttggatc aggccggtct
      841 gccatagaaa caatggaata agcattctac ccaaccggcc gttgccatca agaaacgggt
      901 gcaacgcctc gaattcggca tgaagcaagg caagctgaac caatttgtcg ggggtgtcag
      961 agtgcagata cttctcccat gatgacagag cggcggggag tttgtcggca gaaatcggaa
     1021 caaatctggc ttgttcaatg gtacagccct ttggaccgat ccagtttggt ccagttcgca
     1081 gttcaccggg actattcccc tggcctctga cacctttaag cagttcacga tgagcatcgc
     1141 gaatcactcg catgcagagc ggtaacttct cgagagcagt ctctgcaata tgcatggcag
     1201 aacggtagtt gagaacttca agaatgtcgg cctgacgatc agctgaaaat tcgcggtcac
     1261 cctcggcctc gtactcaagt acttcgccca tggtggcttg aatcccctca atgcgtgatg
     1321 agagtacggc ttcctgggtc gtaagcggac tgaggagcac agaaacattg ggtattgcgg
     1381 aaagggaacc gtcatagcgg gcgacagctg ctgatgccgg tccaatgagt ggtatcagtt
     1441 tctgccaatc aattgttgct ggcggaaatc cacccaaatg ataccttgtt ggtctggcgt
     1501 gatcgtcgcg ttt
//
LOCUS       DAORHD010001344         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_114619_length_1509_cov_2.938102, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001344 DAORHD010000000
VERSION     DAORHD010001344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114619_length_1509_cov_2.938102"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..508)
                     /locus_tag="PKO06_21505"
     CDS             complement(17..508)
                     /locus_tag="PKO06_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72299.1"
                     /translation="MKRFLLLIALFSSLSAFAVEIPPDYLDEVNILQHRAFNANAVRA
                     FVYNVYFLFDKHVPVEEFQQHLVDKDLNMKFPEAHLKSHVDFQNWYKGVGDSIQSNTH
                     TVESIAVKMNEDGTFGVHVIVLWQAINKKGEYLKFKADQNWVLVEDNGRLKIRDYLVK
                     EAK"
     gene            complement(731..1135)
                     /locus_tag="PKO06_21510"
     CDS             complement(731..1135)
                     /locus_tag="PKO06_21510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72300.1"
                     /translation="MELADPGFQIQGIPENLVIVYDSGHTHAMYGRTRYVVTADGKGK
                     KYEIRGVMSSDTEPTSTFSISPEGLKRIVDTINRTRFFKVAPNHENPNISGGSSHYIS
                     VQAGSVSHMVVARMTSVPAIDEISKAIFAEMR"
     gene            complement(1246..1398)
                     /locus_tag="PKO06_21515"
     CDS             complement(1246..1398)
                     /locus_tag="PKO06_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72301.1"
                     /translation="MSHSATMCNNRVVRWVWLMVAILLVPAFAHGAESDVESVKSLVS
                     ELYEIV"
BASE COUNT          346 a          422 c          386 g          355 t
ORIGIN      
        1 cggtctcgcg gtcagcctat ttggcttctt tcacgagata gtctcgaatc ttgagccggc
       61 cgttgtcttc gaccagaacc cagttctgat cggccttgaa cttcaggtat tcgcccttct
      121 tgttgatcgc ctgccagaga acgatgacgt gaaccccaaa ggtgccgtct tcgttcatct
      181 ttaccgcgat cgactcgacg gtgtgagtgt tgctctgaat tgagtcgccc actcctttgt
      241 accagttctg gaagtcgaca tgggacttca gatgagcctc agggaacttc atgttgagat
      301 ctttgtcgac gaggtgttgc tgaaactctt cgacaggcac atgtttatcg aacaggaagt
      361 acacgttgta gacgaaggcc cggaccgcgt tcgcattgaa ggcgcggtgt tgcagaatgt
      421 tgacctcatc gaggtagtcc gggggaatct ccaccgcaaa agcgctaaga gagctaaaca
      481 gagcaatcaa gagcagaaag cgtttcatgg gaactccttg ggttggtgaa cgatcggtta
      541 accacaatca atgaacgacg gcggatacga gccgcgaatc atgtcagcgg aggacgcctg
      601 gcagactgac gacaacgctc aaaaccatag cacaaaactc ccctctcggc tgaagtgctg
      661 ggaaccaagt aggacgtcag tcaacgcggg cggcgggccg atcggctcgc cgcccgcgtt
      721 tttttacagg tcagcgcatc tccgcaaaaa tggctttgga aatctcgtct atagccggaa
      781 cggaggtcat ccgggctaca accatgtgcg acacggatcc ggcttgaaca gaaatgtaat
      841 gagaactgcc cccgctgata ttcgggttct catgattggg cgcgactttg aaaaagcggg
      901 tgcgattgat ggtgtcaacg attctcttca acccttcggg cgaaatggag aacgtgctcg
      961 tcggctctgt gtcagaactc ataacaccac gtatttcgta ctttttccct ttgccatcgg
     1021 ccgtgaccac ataacgggtt cgaccataca tggcatgagt atgtccacta tcgtagacaa
     1081 tcaccagatt ttccggaatt ccctgaatct ggaagcctgg gtcggcaagc tccattcctc
     1141 cgtagatcaa gacgccgatg agcagaacca ccagcaatcc ccggattttc ttttccagct
     1201 ttccctcctc tctcgcgctt ctccgtcgcg acatcacatc tcttgtcaga cgatctcgta
     1261 cagttcactg accaggctct tcacgctttc cacgtcagat tcagcaccgt gagcgaaagc
     1321 cggaaccagc aagatcgcca ccatgagcca cacccatcga acaacccggt tgttgcacat
     1381 ggtggcagag tggctcaccg gccgatctca tcttcggcga tgacgatgat gcgatcccac
     1441 tgggagaagg tcactttctc gctcttctcg gggttgatca ccacgccgta gtttttctcg
     1501 gcatcgaac
//
LOCUS       DAORHD010001345         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_114813_length_1507_cov_5.362259, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001345 DAORHD010000000
VERSION     DAORHD010001345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114813_length_1507_cov_5.362259"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>1507)
                     /locus_tag="PKO06_21520"
     CDS             complement(49..>1507)
                     /locus_tag="PKO06_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72302.1"
                     /translation="NGNPLGIKTKSGVILYRGTCSLLVRDKTPPAVISLNAGALNSFS
                     LKGATGDPLSATGGNPANLTIIVRDNNPFANLNETCPISGIGFHNRQLRQTATVVHET
                     NGSAKTACPITTLASFTSTAGVTMAQFHTALTTNAAAAPTANVTIGSRVITAAQMRQI
                     ASYSSPTLAQQSDALVSNNYRLKRTLRTVPAGSTFSEIEYQLPITDLQHFQGMTATQS
                     SSVAYHANLPVNFANNQTGYNNPGSGLWVNTAAHDGYGVNFVACDSSGNEMGSAAGLT
                     FIDVVDNRHPVLCLSITDLKTNTTHMVPHNIEPNLTNHDFFVKALTQAHTWEPGTDGV
                     FLNCFNPPFGTKIYGLPKPALAALTGLDGLFPTVISRGIEAGVEVDFNLLTNDNIQVA
                     GTPAPYFRIVGPDASSMYYAAGSTSETVPGSSVNQFRHVFRQPGVYDVTLYAEDTALD
                     YNKTAKRNTRTVRFGFVIVPTTLDIRVIDRETNRR"
BASE COUNT          264 a          394 c          520 g          329 t
ORIGIN      
        1 aagaaaaccg gcggccttgt ctggccgccg gtttattctc agagcttctc agcggcggtt
       61 cgtttcgcgg tcgatgacgc ggatatcgag cgtcgtcggc acgatcacga agccgaaacg
      121 aacggttcgc gtgttgcgct tggcggtctt gttgtagtcg agcgcggtat cttcggcata
      181 cagggtcaca tcatagacgc cgggttgtcg gaagacatgg cggaactgat tgacactgga
      241 tcccggcact gtctctgagg tcgagcccgc cgcgtaatac atgcttgatg catcaggacc
      301 cacgatgcgg aagtacggag ccggtgtccc tgcaacctgg atgttgtcgt tcgtgagcag
      361 gttgaagtcg acttcgacgc cggcctcgat accgcggctg atcaccgtcg ggaacaggcc
      421 atcgagaccg gtgagcgcgg caagagccgg tttgggcaga ccgtagatct tggtgccgaa
      481 cggcgggttg aagcagttca ggaacacacc gtccgtgccc ggttcccagg tgtgggcctg
      541 cgtgagcgct ttcacgaaga agtcgtgatt cgtcagattg ggctcgatgt tgtgcggaac
      601 catgtgtgtc gtgttggtct tcagatcggt gatcgacaga cacagaaccg ggtgacgatt
      661 gtcgacgacg tcgatgaacg tgagacccgc ggcactgccc atttcattgc ccgaactgtc
      721 gcaggccacg aagttcacgc cgtagccgtc gtgagctgcc gtattcaccc acaggccgct
      781 gccggggttg ttatacccgg tctgattgtt cgcgaagttc accggcaggt tggcatgata
      841 cgcgacagac gaggactgcg tagccgtcat gccctggaag tgctgcagat ccgtgatggg
      901 cagctgatac tcgatctcgc tgaaggtcga tccggctgga actgtgcgca gtgtgcgttt
      961 gagacgatag ttgttcgaga cgagcgcgtc agactgctgg gccagcgtgg gcgacgagta
     1021 actggcgatc tggcgcatct gggcggcggt gatgacgcgt gacccgatcg tcacgttggc
     1081 tgtgggcgcg gctgcggcat tggtcgtcag cgcggtatgg aactgggcca tggttacccc
     1141 ggccgtcgag gtgaagctgg cgagcgtggt gatggggcag gcagttttgg ctgacccgtt
     1201 cgtttcgtgc acgacggtgg ccgtctgccg cagttgtcgg ttgtggaagc cgataccgga
     1261 gatcggacag gtttcgttca ggtttgcgaa cgggttgttg tcccggacaa tgatggtgag
     1321 gttcgccggg ttcccgccgg ttgcgctgag cgggtcaccg gtggctccct tgagcgagaa
     1381 gctgttcaga gcaccagcat tcaggctgat gacggctggc ggagtcttgt cgcgcaccag
     1441 cagactgcag gtgcctcggt acaagatgac gccggacttg gttttgatgc ccagcgggtt
     1501 tccgttg
//
LOCUS       DAORHD010001346         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_114872_length_1507_cov_2.686639, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001346 DAORHD010000000
VERSION     DAORHD010001346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114872_length_1507_cov_2.686639"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PKO06_21525"
     CDS             <1..392
                     /locus_tag="PKO06_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HNV72303.1"
                     /translation="TLTKRAPTAEEMLDIKYGYEIAKKIADLDIGQTVVVKNRAILAV
                     EAIEGTDEAILRGGKLGNGEVTVVKVARPKQDMRFDLPVVGLDTIHTLMQVKARCLAF
                     EEKKTLLLDRNELIALADSADLAIVVI"
     gene            409..1353
                     /locus_tag="PKO06_21530"
     CDS             409..1353
                     /locus_tag="PKO06_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV72304.1"
                     /translation="MEQSPIRIGVIGIGHLGRHHARILNELPGAKLVGVVDTNEKIAQ
                     ETGEKYGVPWYTDYRKLENQVDAISIVVPTTVHYEVAKFFLDRGVHSFVEKPITKTLD
                     EANDLLLISNRKQLVLQVGHVERFNAAIIKLQSLTDQPRFIECQRMGPFPKRASDVSV
                     VLDLMIHDIDIVLSLVKSKIERIDAVGIPVLTENEDIANAHICFANGCIANLTASRVS
                     EEVIRKLRIFEMDKYLSLDYANQAITLRRKMDQEQIATEELEVHKAEPLRLELAHFIQ
                     CVCERRKPLVSIEDGKNALEVALEILSKIKAATPRQGS"
BASE COUNT          331 a          457 c          419 g          300 t
ORIGIN      
        1 gaacgctgac caagcgcgcc ccgaccgccg aagagatgct cgatatcaag tatggttatg
       61 agatcgccaa gaagatcgcc gatctggaca tcgggcagac ggtggtggtc aagaaccgcg
      121 ccattctggc cgtcgaggcc atcgaaggca cggatgaagc cattctgcgc ggcggcaagc
      181 tcgggaacgg agaagtcacc gtggtcaaag tggcgcgtcc gaagcaggac atgcgcttcg
      241 atcttcccgt cgtcggcctc gacacgattc acaccctgat gcaggtcaag gcccgttgcc
      301 tggccttcga agagaagaaa acgctgctgc tggaccgcaa tgaactgatc gcgctggccg
      361 actcggctga tctggccatc gtcgtgattt aacccgggag aggaacccat ggaacagtca
      421 cccattcgca tcggagtcat cggtatcggc catcttggtc ggcatcatgc ccgcattctg
      481 aacgagttac ccggcgccaa actggtcggc gtggtcgaca cgaacgagaa gatcgcccag
      541 gaaaccggcg agaagtatgg tgttccctgg tataccgatt atcgcaagct cgagaaccag
      601 gtcgacgcca tttcgatcgt cgtaccgacg acggtgcatt atgaagtagc caaattcttt
      661 ctggaccgcg gcgttcactc gttcgtcgag aagccgatca cgaaaaccct cgacgaagca
      721 aacgatctgc tgctgatttc gaaccgcaag caactggtac tgcaggtcgg acacgtcgaa
      781 cgattcaacg cggccatcat caaactgcag tctctcaccg atcagccgcg attcatcgag
      841 tgtcagcgca tggggccttt tccgaagcgc gcgtcggatg tcagcgtcgt gctcgatctg
      901 atgattcacg atatcgacat tgttctgtct ctggtcaaat cgaagattga acgcattgac
      961 gccgtcggca tcccggtact gaccgagaac gaggacatcg ccaacgccca catctgcttt
     1021 gccaatggct gtatcgccaa tctgaccgct tcccgcgttt ccgaggaagt gattcgcaaa
     1081 ctgcgcatct tcgagatgga caagtatctg tcgctcgatt acgcgaacca ggccatcacc
     1141 ctgcgccgca aaatggacca ggaacagatc gccaccgagg aactggaagt gcacaaagcc
     1201 gagccgttgc gtctcgaact ggcccacttc attcagtgcg tgtgtgaacg ccgcaagccg
     1261 ctggtctcga tcgaagacgg caagaacgcc ctggaagtgg cactcgagat tctctcgaag
     1321 atcaaggccg cgacaccccg gcagggttcc tgaacgtttc gctctccgtg acttcttccg
     1381 ccacactcgc cccaaacgcc cattcgctgg gggcgtggtt ctttcggtac cgggatctgc
     1441 tcccgattcc gctcgttctc ggtctgtgcc gcacagctcg cccacgtgcc gtcacctggc
     1501 tgttcgg
//
LOCUS       DAORHD010001347         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_115085_length_1505_cov_3.821379, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001347 DAORHD010000000
VERSION     DAORHD010001347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115085_length_1505_cov_3.821379"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="PKO06_21535"
     CDS             <1..>1505
                     /locus_tag="PKO06_21535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72305.1"
                     /translation="HLPGVTDFPRLSKHLVSLSDSLSVLPGTINVVDGERIKSSEFVN
                     LLKICRKYFDYIVIDTESRIEDIFLNALDVSDRILFLVDPTDIFTIKCASRYFAGIRK
                     LNYADERMAIVATKVDESFVPDQLEKMLKLPVFGALPKMAGYTPEYGVTPFQKLPNSS
                     YVQFVRKVSTVLLNEASAGTKTPSGFLSRLFFGSTANSEKGVAKAEESETCGLDFPNI
                     TPENLRVLMKYVRVTYLNGKFEEARRQTQHILTYCPKSALVYQVLGEIFQAEGSISDA
                     IECYRKAMDLDPQNHYATGQLGILGNDEKLFNRAVQLVNTKIQEHPRYPDLHKDLGVL
                     YLNRRMIDPAIAAFKKALEVNPNYGEARVHMADALGHLGRYPEAIDSLSAVKQKTIRV
                     YYLLGNYLHTLGRFHEAIDAYRMVLKINPSYEDTARRLDKLESYFEKVQSLIEMHEDI
                     IRTRPSFPDIHMKLGMMYAVVGRRSEAEKAFRKALELNPQYEDARRYLDQL"
BASE COUNT          354 a          436 c          391 g          324 t
ORIGIN      
        1 catctccccg gtgtcacaga cttccctcgc ctgtcgaaac atctggtcag cctgagtgat
       61 tctctcagcg tcctacctgg caccatcaac gtcgttgacg gagagcgcat caagtcaagt
      121 gagttcgtta atcttctgaa gatctgtcgc aagtatttcg actacatcgt gatcgatacg
      181 gaaagtcgga tcgaagacat attcctcaat gcgctcgatg tctcggaccg catcctcttc
      241 ctggtcgatc caaccgacat cttcacgatc aagtgcgcgt cgcgctattt tgcgggcatt
      301 cgcaagttga attatgctga cgagcgcatg gctattgttg ccaccaaggt cgatgaatct
      361 ttcgtccccg accagctcga gaaaatgctg aaactgccgg tgttcggggc tctgccaaag
      421 atggccgggt acacgcctga gtatggggtg acaccgttcc agaagctgcc caactcgtca
      481 tatgtgcagt tcgtgcggaa ggtgtcgacg gttctgctca acgaggccag cgccggaacc
      541 aagactccga gtggttttct cagtcgtctg ttcttcggca gcaccgcgaa ttccgagaag
      601 ggggtcgcca aggccgaaga atcagagacc tgtggtctcg acttccccaa catcaccccc
      661 gaaaacctgc gtgtgctgat gaagtatgtg cgcgtgacct atctgaacgg aaaattcgaa
      721 gaagcccggc gacagacaca gcacattctc acgtattgtc cgaagtcggc actcgtgtac
      781 caagttctgg gagaaatttt ccaggctgaa ggaagtatct cggacgccat cgagtgttat
      841 cgcaaggcga tggatctcga tcctcagaac cactacgcca cggggcagct cggtattctc
      901 ggtaacgacg agaagttgtt caatcgggcc gtacagctgg tgaacaccaa gattcaggaa
      961 catccgcgtt accctgacct gcacaaagat ctgggggtcc tgtatctcaa tcgtcgcatg
     1021 atcgatcccg cgattgccgc cttcaagaaa gctcttgaag tgaatccgaa ctacggcgaa
     1081 gcccgtgttc atatggcaga cgctctgggt cacctcggac gatacccgga agccatcgac
     1141 tctctctcgg ccgtcaagca gaaaaccatt cgggtgtact atctgctcgg caactacctg
     1201 cacacgctgg gccgattcca cgaggccatc gatgcctacc gcatggtcct gaagatcaat
     1261 ccgtcctacg aagatacggc gagacgtctc gacaaactcg aaagctactt cgaaaaggtg
     1321 cagtcgctca tcgagatgca cgaagacatc attcgcaccc ggccgtcgtt ccccgatatt
     1381 cacatgaagc tcggcatgat gtatgccgtg gtgggtcggc gctcagaagc ggagaaagcg
     1441 ttccgcaagg ccctcgaact caacccccag tatgaagacg cccgacgcta cctcgaccag
     1501 ctcag
//
LOCUS       DAORHD010001348         1503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_115384_length_1503_cov_3.267956, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001348 DAORHD010000000
VERSION     DAORHD010001348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115384_length_1503_cov_3.267956"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="PKO06_21540"
     CDS             complement(<1..1111)
                     /locus_tag="PKO06_21540"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNV72306.1"
                     /translation="MVEEVRGVPVEEVKDTPSIDEERELDELLDLDRHKLSHIPKKTP
                     TASERWMKYLGFPVGLLLFLWIYFSPVPAGLTGAGQAVIASFTLALVWWVCEPIPTYV
                     TSLVLMLLLVFLDAWDQKQVLAVLGLDVIWLNVMAFILSSILVKTNLAKRVALALIVR
                     FGTSSGRILLAFVGLQLLLAPMIPATAARTVMTLPLMLVVAAIFGATSDTPNNFGRNL
                     FLQNLLGINIFSSGFMTGSAANLIALMFLDSMAGQKVYYSDWMLGSLPIVVTAMLIAW
                     YVGPRWLFPLAPSDQTPHIKGGIPALQRQLERMGPVSFAEKKAAAIFGLVIFLWVTDR
                     FHLGWFGFEIDPAMAAMLGAIICFLRSVRKSSGICV"
BASE COUNT          380 a          434 c          421 g          268 t
ORIGIN      
        1 ctacacaaat gcccgacgac ttccggacgc ttcgcagaaa gcagatgatc gcccccagca
       61 tagccgccat tgccgggtcg atttcgaagc caaaccaccc cagatgaaag cggtctgtca
      121 cccagagaaa gatcaccaga ccgaaaatgg ccgcggcttt cttttccgca aacgacaccg
      181 gacccatgcg ctcgagctgt cgttgcagcg ccggaatacc ccctttgatg tgaggggtct
      241 ggtcgctcgg cgccagcgga aacaaccagc gcggaccgac ataccaggca atgagcatgg
      301 ctgtcacgac gatcggaaga ctgccgagca tccaatccga gtagtacact ttctggccag
      361 ccatggaatc aagaaacatc agtgcgatca ggtttgcggc acttccggtc atgaaaccgg
      421 aagagaagat gttgatgccg aggagattct gtagaaacag attgcgacca aagttgttcg
      481 gcgtgtcact ggtcgcgccg aaaatggccg cgacgaccag cattaacggc agagtcatga
      541 ctgtacgagc cgccgttgcc ggaatcatag gcgccaaaag cagttgcagt ccaacgaatg
      601 cgagaagaat tcttcctgaa cttgtcccga aacgcacgat gagtgccaac gccacgcgct
      661 tcgccaggtt cgtcttgacc agaatcgaac tgagaatgaa agccatcacg ttcaaccaga
      721 tgacatccaa tccgagaacc gccagaacct gcttctggtc ccaggcgtcg agaaacacca
      781 gcagcagcat gagaaccagc gacgtcacat aagtgggaat cggttcacag acccaccaga
      841 ccaacgccag agtgaaggaa gcgatgaccg cctgtccggc acctgtcagc ccggccggga
      901 ccggcgagaa gtagatccag aggaagagga gcagcccgac cggaaagccc aggtatttca
      961 tccagcgctc gctcgcggtg ggggtctttt tcggaatgtg agacagttta tgtcgatcga
     1021 ggtcgagcag ttcgtccagt tctcgctctt cgtcgatgga cggcgtgtcc ttcacttctt
     1081 ccacgggcac tccacggacc tcttcgacca ttgaattccc ccccgtgaca acctcgaccc
     1141 ggcttctgcg tcggctcatt gtaggtacaa ttctcgcgac atgcaaccgc aattccagcc
     1201 ccgttcacag accgtgaaaa acggtttccg cgaaatccgc tgaagagcgt agcatctgag
     1261 ttcggactta gagtagaatc acgcgatgac gcaggaatca cgagacgaca ggagggggta
     1321 acgccatggg cgacaagcag aagaacggca acgtgatcgg gttcgatctc ggcggcacga
     1381 agatgatggc ttccgtgctt gatacggaat tcaagatcgc gggggccaag cgagagaagc
     1441 tgcgcgatcg cggtgggcag aaactcgggg tcgagcagat cattcaactg gcccgttccg
     1501 ctc
//
LOCUS       DAORHD010001349         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_115777_length_1500_cov_3.035294, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001349 DAORHD010000000
VERSION     DAORHD010001349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115777_length_1500_cov_3.035294"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="PKO06_21545"
     CDS             complement(<1..380)
                     /locus_tag="PKO06_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72307.1"
                     /translation="MNRLSWRIGSWLFLLGAMLVLWPQTADARRGGAVFDPNESRPIS
                     SGNAYNQPVPVATHVPALNRQKPRQMSGLEQGIWTAVKSLGSATGSTLGASVGMVAVP
                     AAVTAIVGALGISTSGAGFVSGVL"
     gene            complement(446..>1500)
                     /locus_tag="PKO06_21550"
     CDS             complement(446..>1500)
                     /locus_tag="PKO06_21550"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72308.1"
                     /translation="YLAQKRRIRSAQEESSIWDGVEQYAYVTPVTFLASFLVATTEDL
                     GHVEIALKKGYARLYAGMGVMTLLAIMLGIILSGGLVRPITNLDQAFGEVRDGNLAVT
                     LPDMGSDEIGRLGATFNTMVAQLRERERMRAYVSGSVLEKVRDEHVETGQTAQFRPAA
                     ILFSDIRGFTSLSEKYPPAQIFEMLNGFLGGVEAIIREHQGDVDKFIGDAVMAVFLGD
                     EKQIACSAVEAALDMRRFLAQFNRERQAAGSFPIEIGIGINVGNVLWGDIGSTRRKDS
                     TVIGDEVNLASRLETASKLGRHTHIVVSESTSVLVADIVELEEMSVREVKGKEQSVRM
                     FEVVACRPAPRHSSSG"
BASE COUNT          315 a          459 c          411 g          315 t
ORIGIN      
        1 atgagtaccc ctgacacgaa gccagcaccc gaggtcgaaa tacccagtgc gccgactatg
       61 gcggtcacgg ctgccggcac ggccaccatg ccgaccgagg ctcccagcgt cgaaccggtg
      121 gctgagccga gcgacttcac cgcggtccag attccctgtt ccaatccgct catttgtcgt
      181 ggcttttgtc gattgagagc cggtacatgc gtggcgaccg gaacgggttg attgtaggcg
      241 ttgccactgc tgatggggcg cgactcgttc ggatcgaaca ccgccccgcc acgacgggcg
      301 tcggccgtct gtggccacaa cacgagcatg gcaccgagca agaacaacca agaaccgatt
      361 cgccacgaca gtctattcat gggtcaactc cagggaaggc gtttccctca ttgtatccgt
      421 ttttccgtga tcagagaagg gagcgtcatc cggaactcga atggcggggt gctggccggc
      481 aggcgacaac ctcgaacatt ctgacgctct gttctttccc tttcacttcg cgaacgctca
      541 tctcttctag ttctacaatg tcggcaacca ggacagaagt gctctcggag acgacgatgt
      601 gcgtgtgtct tccgagcttg gaggcggtct cgagacgtga agccaggttc acttcatcgc
      661 cgatgacggt cgaatccttc cggcgcgtgg acccgatgtc gccccagaga acattgccaa
      721 cgttgatgcc gataccgatc tcgatgggga aagagccagc agcctgacgt tcacgattga
      781 attgcgccag gaaccgtctc atgtcaaggg cggcctcaac agcggaacac gcgatctgtt
      841 tttcgtcacc gagaaacacg gccatgacgg catcgcctat aaatttatca acatcgccct
      901 gatgttctcg tatgatggct tcgaccccgc caagaaaccc gttcagcatt tcgaaaatct
      961 gcgccggcgg atatttttcc gacaacgatg taaagccgcg aatgtccgag aagagaatgg
     1021 ctgccggtct gaattgagcc gtctgtccgg tctcgacatg ttcatcgcga actttctcga
     1081 ggacgctgcc tgacacatac gcccgcatgc gttctcgctc gcgcagctgt gccaccatgg
     1141 tattgaacgt cgcgcccagt cgcccgatct cgtcacttcc catgtccggc aaggtaacgg
     1201 cgagattgcc gtctcgaact tctccgaacg cctgatcgag attcgtgatc ggtcgcacca
     1261 gaccgccgga aaggatgatc cccagcatga tcgccagcag cgtcatcact cccatcccgg
     1321 catagaggcg tgcgtatcct tttttgaggg cgatctcgac atgccccagg tcttccgtcg
     1381 tagccacgag aaacgaagcg agaaaggtca ccggtgtgac gtaagcatac tgttccacgc
     1441 catcccagat cgacgactct tcctgggcac tgcggatccg tcttttctgc gccaggtagg
//
LOCUS       DAORHD010001350         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_115797_length_1500_cov_2.823529, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001350 DAORHD010000000
VERSION     DAORHD010001350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115797_length_1500_cov_2.823529"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1500
                     /locus_tag="PKO06_21555"
     CDS             <1..>1500
                     /locus_tag="PKO06_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72309.1"
                     /translation="FPILVLQVAFRFAEQQEVATSITRRHQAHERHLQRLLRLDQDSE
                     LVCRSLRRGFRKLVQLPSQDRHRFLQYLARLFPGNFEAYYFDQSGHRIDALSASAFPR
                     RASEMAYAAIGKKSRSEAITPSEEKLLATMFEVPSLSQFTNSQGKTVPLLSRDRMGAI
                     TWDLTPASASLEIGGFFAIIHPGTIEPDRAVRSALRSLNKRSGAVQFGVLNLQDDHFA
                     AYPPELNQKEDLRLRVLSALSVYERAFRGPGYHGLLAPRSRGGYVVSLSPLPRLFPLW
                     FWAAFNLICVIWLVVVLVRVVRGQDVFTGKITARMIGLFLFAVGSPSLVLLFGGYYAL
                     RDHSSILMQNLEVQVRDKLTQLDQRLPSEVKRIEKHLTRLMTKARGETDLTRRIELFR
                     TLEQEPWCEQAYLVNQKGESVYSRRDMNDPVFRDRSRFPLMLCREILRRYNKLYAIDA
                     GTLMVETTTGILDQLMGSKDKVNIDQIIAQAGQFTYMSMFDESAFFFIDS"
BASE COUNT          283 a          445 c          419 g          353 t
ORIGIN      
        1 ctttccgatt ctggtgctgc aggtggcctt tcgctttgcc gagcagcagg aagtcgccac
       61 ctcgatcacc cgtcgccatc aggcacatga acgtcatctg cagcggttgc tgcgactcga
      121 tcaggacagc gaactggtct gcagaagcct gagacgcggg tttcgcaaac tggttcaatt
      181 gccttcgcaa gaccgacacc gtttcctgca gtatctggcc cggctgttcc ccggcaactt
      241 cgaagcctat tattttgatc agtcaggtca tcgcatcgat gcgctttccg cctccgcgtt
      301 cccccgccga gcgtccgaga tggcttatgc cgcaatcggc aagaaatcgc gatctgaggc
      361 tatcaccccc agcgaagaaa aacttctggc gaccatgttc gaagtaccct ccctgtctca
      421 gttcaccaac tcccagggga aaaccgttcc gttgttgtcg cgagaccgca tgggagcgat
      481 cacctgggat ctgacgccgg caagtgcaag cctcgagatc gggggattct tcgcgatcat
      541 acatccgggc acgatcgaac ccgatcgcgc cgttcgcagt gcgctcagat cgctcaataa
      601 gcgttctggt gccgtgcagt ttggggtgct caacctgcag gatgaccatt tcgccgcgta
      661 ccccccggag ctgaatcaga aggaagatct gcgcttgcgc gttctgagcg ctctcagcgt
      721 ctacgaacga gcttttcgcg ggccggggta tcacggtctt ctggctccgc gctcgcgtgg
      781 cgggtatgtg gtttccctgt ccccacttcc ccgtcttttt ccgctctggt tctgggcggc
      841 gttcaacctg atctgtgtca tctggctggt cgtggttctg gtgcgtgtcg tgcgcggaca
      901 ggatgtcttc accggcaaga tcacggcccg catgatcggc ttgtttctct ttgccgtcgg
      961 gtctccctcg ctggtgctgt tgttcggtgg gtattacgct cttcgcgatc acagcagcat
     1021 tctgatgcag aacctggaag tccaggttcg tgacaagctg acacagctcg atcagcggct
     1081 tccgtcagag gtcaaacgga tcgagaagca tctgaccaga ctgatgacca aggctcgcgg
     1141 tgaaaccgat ctcacccggc gtatcgagct ctttcgcacc ctcgaacagg aaccctggtg
     1201 tgagcaggcc tatctggtca accagaaggg ggaaagcgtc tattcgcggc gagatatgaa
     1261 cgaccccgtt tttcgcgatc gatcacggtt tccgctgatg ctgtgccgcg aaattctgcg
     1321 tcggtataac aagctctacg cgatcgatgc tggaacgctg atggtcgaga caaccaccgg
     1381 gattctcgac cagctcatgg gttcgaagga caaggtcaac atcgaccaga tcatcgcgca
     1441 ggcgggtcag ttcacctata tgtcgatgtt cgatgaatcg gcctttttct tcattgactc
//
LOCUS       DAORHD010001351         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_116157_length_1497_cov_2.820388, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001351 DAORHD010000000
VERSION     DAORHD010001351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116157_length_1497_cov_2.820388"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKO06_21560"
     CDS             <1..299
                     /locus_tag="PKO06_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72310.1"
                     /translation="PPPSRGTLTPKQGRDKERRHSDGFLSGHLCGFVGEAFFDVRCGS
                     EGYKAIISKASVSGQDVGDAFAFHEHEARAIYQVDVATISELEAAENLLWSFSS"
     gene            381..>1497
                     /locus_tag="PKO06_21565"
     CDS             381..>1497
                     /locus_tag="PKO06_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72311.1"
                     /translation="MIQKLQKRVWAILRANTTTVVLCVLVLLFHLWAETAGAADEGWS
                     GMVIVDSSIPLHNEGSREAIPLPYPLVLYVDPTSEAYKKGFRAGDFIIGPPERELLSI
                     STLMETLKSSPEALRIRNKDGRKVLRFVPSPVTAEGKILIQMQEELLAAGKNPPDARK
                     MVLLGAQAPECAAAAFLVGYTAMRNSDEKAAELWFNKTTEKNTDPFLGSLAVRIAPSG
                     MRSSLQGALSKLQVESDDSARGDAQKDAGGKTGTAPAPDPVASDQSGIKTVNMNLAMT
                     GTTVPETIETLREAHQKKPNDQTIREKLMFALLRKVNGNQSSPMEELEIYLREADDMV
                     PNSFYVNHLWGSLYMSRENFEPAAAKLESAVAAQPEHL"
BASE COUNT          355 a          377 c          453 g          312 t
ORIGIN      
        1 tgccaccgcc ctcccgtgga actttgacac cgaagcaggg acgtgacaaa gaacggcggc
       61 attcggatgg ttttttgtca gggcatcttt gcgggtttgt cggtgaggcg ttttttgacg
      121 tccggtgcgg ttccgagggt tacaaagcca tcatcagcaa agcgtctgtg tcaggtcagg
      181 acgtgggtga tgccttcgct ttccatgaac acgaagctcg cgcaatctac caggttgatg
      241 tcgcgacgat ttcggagctc gaggcggcgg aaaaccttct ctggagtttc tcaagctgag
      301 gaataaaaag ccatcgggat gaggggcttt gcgtgcgaag cgggaatcgc gtatcctgaa
      361 gagagaatgg agtggttgcc atgatacaga aacttcagaa gcgagtttgg gcgattctgc
      421 gagcgaacac gacgacagtc gtgctctgcg tgctggttct gctgtttcac ctgtgggcag
      481 agactgccgg agccgctgat gaaggctgga gcggaatggt gatcgtcgat tcttccattc
      541 ccctgcataa cgaaggcagt cgcgaagcga ttcccctgcc ctatccactg gtactctatg
      601 tcgatcccac ttctgaagcc tataagaaag gttttcgggc gggtgacttt attatcggtc
      661 caccggaacg tgaacttctg agcatctcaa ccctgatgga gacgctcaaa tcaagccctg
      721 aagcgttgcg catcaggaac aaagatggcc ggaaggtgct tcggttcgtt ccctcccccg
      781 tcacggccga aggcaagatc ctgatacaga tgcaagaaga gttgctggcg gcagggaaaa
      841 acccaccgga tgcccggaaa atggttctgc tgggagcaca ggctccggaa tgcgcggctg
      901 cagcgttttt ggtcgggtat accgccatgc ggaatagtga tgagaaggcc gccgagttgt
      961 ggttcaacaa gacgaccgaa aagaacaccg acccctttct cggaagtctc gcggtgcgca
     1021 ttgctccctc tggcatgcgt tcaagtcttc agggggcact ctcgaagctg caggtcgaga
     1081 gcgatgactc agcacgcgga gacgcgcaga aggacgcagg cggaaaaact ggaactgcgc
     1141 cagcgcctga tccggttgct tcagatcaga gcggaatcaa gacggtcaat atgaacctgg
     1201 caatgacggg aacgactgtg cccgagacca ttgaaacgct gcgagaggcg catcagaaga
     1261 aaccgaatga tcagacgatc agagaaaaac tgatgtttgc tttgctgcgc aaggtgaacg
     1321 gcaatcagag cagtcccatg gaagaactgg aaatctacct gcgtgaagcc gacgatatgg
     1381 tgccgaacag cttctacgtg aatcatctgt ggggctcgct ctatatgagt cgcgagaact
     1441 ttgaaccggc cgcggccaaa ctcgagagtg ccgttgcggc tcagcctgag catcttg
//
LOCUS       DAORHD010001352         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_116405_length_1495_cov_3.499306, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001352 DAORHD010000000
VERSION     DAORHD010001352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116405_length_1495_cov_3.499306"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PKO06_21570"
     CDS             complement(<1..1090)
                     /locus_tag="PKO06_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV72312.1"
                     /translation="MRLWLAQRQISHVRAHREAVPESFSGHIALGAHQKAADYAVARA
                     RFGRVGLAIEVAVLLGLTFGGGLQSLNDFWSPRLDGLPYGVALVFSVIALSAVVDLPL
                     TLYHQFVIEEKFGFNRMRLTLFLGDLVKHAALGIVIGTPVLLAVLWLMESMGELWWLY
                     VWLFWCGFNLLLLFIYPTWIAPLFNKFAPLEDDALRTRVEALLARCGFTTSGLFVMDG
                     SKRSGHGNAYFTGFGKTKRIVFFDTLLGRLQAVEVEAVLAHELGHFRHRHIVKRIGML
                     FAMSLLFLAILGQLINADWFFNGLGLDARNTALGLVLFFLAGPVFTFFLTPLLSLLSR
                     RDEFEADSYAAGHASAGNLVSALVKLYED"
     gene            1188..>1495
                     /gene="orn"
                     /locus_tag="PKO06_21575"
     CDS             1188..>1495
                     /gene="orn"
                     /locus_tag="PKO06_21575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNV72313.1"
                     /translation="MTQVANHLVWLDMEMTGLEPERDRIIELAMIVTDSQLVTIAESP
                     AWVVSQDAARLDAMDDWNKGTHGRSGLIERVKSSMLDEATVEQKMIDFLADHVPARVS
                     P"
BASE COUNT          371 a          404 c          463 g          257 t
ORIGIN      
        1 tatcttcata cagtttcacg agtgcgctca ccaggttgcc tgccgatgca tgcccggccg
       61 catagctgtc ggcttcaaac tcgtcgcggc gggacagcag gctcaacagc ggggtcagga
      121 agaaagtgaa aaccggtccg gcgaggaaaa atagcaccag tcccagcgcc gtattcctgg
      181 catcaagccc caggccattg aaaaaccagt cggcattgat cagttgaccg agaatggcga
      241 gaaaaagcag cgacatggca aacagcatgc cgatccgctt gacaatgtgc cggtgcctga
      301 agtgcccaag ttcgtgcgcg agtacggctt cgacctcgac ggcttgcagg cgaccgagca
      361 gcgtgtcgaa aaagacgatg cgcttggtct tgccgaaacc ggtgaaatag gcgttgccgt
      421 ggcccgatcg ctttgagcca tccatcacga aaaggccaga agtggtgaag ccgcagcgtg
      481 caagcagggc ttcgacgcgc gttctgagag cgtcgtcttc gagtggcgcg aacttgttga
      541 acaagggtgc gatccaggtc gggtagatga acaggagcag cagattgaag ccgcaccaga
      601 acagccacac gtagagccac cacagctctc ccatgctctc catcagccag aggacggcaa
      661 gcagcaccgg tgtaccgatg acgatgccca gggccgcgtg tttcacgagg tcgccgagga
      721 agagcgtgag gcgcatgcgg ttgaaaccga acttttcttc gatcacgaac tggtgataga
      781 gcgtgagcgg cagatcaacg acggcagaaa gcgcgatcac gctgaatacg agtgcgaccc
      841 cgtaagggag accatcgagg cgaggcgacc agaagtcatt cagtgactgc aggccgccgc
      901 cgaacgtgag gccgagtaga acggcgactt cgatggcgag accgactctg ccgaaacggg
      961 cacgtgccac cgcatagtca gcggctttct gatgggcgcc aagagcgata tgtccagaga
     1021 agctctcggg taccgcttct cgatgcgcgc gtacatgaga aatctgacgc tgcgcgagcc
     1081 atagacgcat gacacacatc aacagcaagg agacaagaaa cagcagtgag aaagtattga
     1141 gcatgggcct gtgacacaat agggctttta cagttcaggt ggattatatg acacaggttg
     1201 ccaatcacct cgtttggctg gacatggaaa tgaccggtct ggaacctgaa cgcgaccgca
     1261 tcattgaact tgcgatgatt gtgaccgaca gccagttggt gaccatcgcc gaatcgccgg
     1321 cctgggtggt tagtcaggat gcggctcgcc tcgatgcgat ggatgactgg aacaagggaa
     1381 cccacggacg ctccggcctg atcgagcggg tgaagtcgtc gatgctcgac gaggcaacag
     1441 tcgagcagaa gatgatcgac ttcctggcag atcacgttcc ggcgagggtc tcgcc
//
LOCUS       DAORHD010001353         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117179_length_1489_cov_3.229428, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001353 DAORHD010000000
VERSION     DAORHD010001353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117179_length_1489_cov_3.229428"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKO06_21580"
     CDS             <1..901
                     /locus_tag="PKO06_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV72314.1"
                     /translation="RERLFAERQKRRRPHRDEKILTDWNGLMIAALAKASWVLAKPDY
                     ADAAAVAARQLLATCQTDDGGLWHRTADGDTGIRAHLDDYAFVIWGLLELYEACFDVF
                     WIQKARQLTDYVITHFLDRDHGGFYFTSDLAEPLLVRSKEIYDGATPSGNSVMAANLL
                     RLGRLTGDPSLEAVTEQLFKTFSQKVSSVPMVHAFLLTALDFAIGQSCEVVIVGNPSE
                     PTTRSLLDVARREYHPNRVLVLKNPDDSTPLENVAPYTRPLSMLDNRPTAYVCHQYQC
                     STPTTDPTLLAEQLRRHHSSSVT"
     gene            917..>1489
                     /locus_tag="PKO06_21585"
     CDS             917..>1489
                     /locus_tag="PKO06_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72315.1"
                     /translation="MTVSLRSIAPRSHRHTLALSVCSFIAFLTLAPALCGQVVIVDDR
                     DPDCQLSGVWADNRSNGFSAAGTYKDGYHYTSSHAPFKRTGKEKAVYSPNLPKAGSYK
                     VEVSFRASSSNRSSKVTYQVMHKGGKTSKVVNQKDGNDMTWAALGTFEFDAGQAPCVA
                     MVSDGGQSASVDAARFTFTAGGGNTGSLGDL"
BASE COUNT          294 a          469 c          403 g          323 t
ORIGIN      
        1 ccgggagagg ctctttgccg agcgccagaa gcggcggcgc cctcatcgcg acgaaaagat
       61 cctgaccgac tggaacggcc tgatgattgc cgccctggcc aaagcctcat gggtcctcgc
      121 gaaacctgac tatgccgacg ccgcggcagt ggcagcccga cagctgctgg ccacctgtca
      181 gactgacgac ggcggtctct ggcatcgcac cgcggatggt gacactggca ttcgtgctca
      241 tctcgacgat tacgccttcg tcatctgggg gctgctggaa ttgtacgaag cctgtttcga
      301 cgttttctgg attcagaaag ccagacagct caccgattac gtcatcaccc acttcctgga
      361 ccgcgaccat ggggggttct atttcacgtc cgacctcgcc gagccgctgc tggtgcgttc
      421 aaaagagatc tatgacggag ccacgccgtc agggaactcg gtcatggccg ccaatctgtt
      481 gcgcctcgga cgtctgactg gggatccatc actcgaagca gtgaccgagc agttgttcaa
      541 gacattttcg cagaaggtca gttccgtgcc gatggtgcat gcctttctgc tcaccgcgct
      601 cgattttgcc atcgggcaat cctgcgaagt ggtgatcgtg ggtaatccct ctgaaccgac
      661 gacacgttcg cttctcgacg ttgcacgtcg tgagtatcac ccgaaccgtg ttctggtgtt
      721 gaagaacccg gacgattcga cacctctcga gaatgtggca ccctataccc gtccactctc
      781 catgctcgac aatcgtccca ccgcgtatgt ctgccatcaa tatcagtgtt ccacgccgac
      841 caccgaccca acccttctgg ctgagcaact acgtcggcac cattcctcgt ccgtcacctg
      901 aattcggagg tgcatcatga ccgtatctct tcgttccatc gctcctcgat ctcaccgaca
      961 cacccttgct ctctcagtgt gcagtttcat cgcgtttctg actcttgctc cggctttatg
     1021 tggtcaggtg gtcattgtcg atgatcgcga cccggactgt cagttgtctg gcgtctgggc
     1081 cgacaatcgt tccaatgggt tctccgccgc cggaacatac aaggacggat accactacac
     1141 cagctcgcac gctccgttta aaagaaccgg aaaggaaaaa gcggtctaca gcccgaacct
     1201 gcccaaggcc ggctcgtaca aagttgaggt ctccttccgg gcatcttcga gcaatcgttc
     1261 gtcgaaagtc acctaccagg ttatgcataa gggtgggaaa accagcaagg tcgtgaacca
     1321 gaaagatggg aacgacatga cctgggccgc gctcggaacc ttcgagttcg acgccggcca
     1381 agcgccctgc gtcgccatgg tttcagacgg cggccagtct gcttcggttg acgccgcccg
     1441 ttttacgttc accgcggggg gcgggaacac cggcagtctc ggcgacctc
//
LOCUS       DAORHD010001354         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117192_length_1489_cov_2.861227, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001354 DAORHD010000000
VERSION     DAORHD010001354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117192_length_1489_cov_2.861227"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKO06_21590"
     CDS             complement(<1..261)
                     /locus_tag="PKO06_21590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72316.1"
                     /translation="MKHDDSLLYDLLNATHACGVPIRGETRKRRQATFIEMRKTEWCK
                     KNCRWGDEKFPGVEPTNDALSGTVSEGVHEGSGLNFGVDALFD"
     gene            253..>1489
                     /locus_tag="PKO06_21595"
     CDS             253..>1489
                     /locus_tag="PKO06_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72317.1"
                     /translation="MFHRHIFIRFVACWFCLLAGCNTLFGLASEYERNGEVYVLVGDG
                     AKIGVYRLNDPAGEGVPTGWLYNPLQSYGISVNLSRKVYTFSETATSTFSLLTGNISV
                     LMKANGANIMDGFHYDHRGNWGELGTPMYTTPGTGKWYVAGWGRENNYHGAGTFSAVT
                     PQCTISSVNVIVPDGAGYGSIPNAQWYQSSDNDTYGRPGKAYRDRVEGAPHDYHLLEW
                     YQGIAANTPADKGVKATSYEKKVQRALLGSCIDPCGTNVASLSMGAATQTVSLALSSF
                     GRTYCYTRTEAMATDGKVTLDGTNYAGPVIGTINNLTTKWIGVSLRNTSADYVYVLGT
                     DVIKSWLTQLGFTIPTGFNVTAVSVSDQWYLKGGIVFAYNNSNGIAYQFTRADNADGT
                     ASGTSYFTSVNLGSGIDDIK"
BASE COUNT          356 a          403 c          380 g          350 t
ORIGIN      
        1 gtcgaagagc gcatcgacgc caaaattcaa cccggaaccc tcgtgaacac cttctgaaac
       61 ggttcccgag agagcatcat tcgtcggttc caccccggga aatttttcat ctccccaccg
      121 acagtttttt ttacaccatt cagtttttcg catctcgata aaggtcgcct ggcgcctctt
      181 tcttgtctct ccccgaattg gcactccgca tgcgtgagta gcatttaata gatcatagag
      241 gagggagtcg tcatgtttca tcgccatatc ttcatcaggt tcgttgcctg ttggttttgt
      301 ctgctcgccg ggtgcaatac cctgttcggg cttgcctcgg aatacgaacg aaatggggaa
      361 gtctatgtcc tggtcggaga tggggcgaaa atcggcgtct atcgcctgaa tgatcctgcc
      421 ggagaagggg tccctaccgg gtggttgtac aaccctcttc agtcttacgg tatcagtgta
      481 aacctcagtc gcaaagtcta caccttttct gaaacggcaa cttcaacgtt cagtttgttg
      541 accggcaaca tcagcgttct gatgaaagcc aacggtgcca atattatgga cggctttcac
      601 tatgaccacc gtggaaactg gggcgagctg ggaacaccga tgtataccac gcccggaacc
      661 ggtaaatggt atgtcgctgg atggggccgt gaaaacaatt atcacggagc cggcactttc
      721 tcggccgtca ccccgcaatg cacgatctct tccgtcaatg tcatcgtacc cgatggtgcc
      781 ggatatggaa gcataccaaa cgcacagtgg taccagtcca gcgacaatga cacctatggt
      841 cggcccggaa aggcttatcg cgaccgtgtg gagggagctc cccatgacta tcacctgctc
      901 gagtggtacc aggggatcgc ggccaatacc ccagctgata agggagtcaa agcaacatcg
      961 tatgagaaaa aagtgcaacg agctctgctt ggttcctgta tcgacccctg cggaacgaac
     1021 gttgccagtc tctcgatggg ggctgccaca caaaccgtca gtctggcttt gtcttcattc
     1081 ggtcgtacgt attgctacac ccgtacagaa gcgatggcca ccgacgggaa agtgaccttg
     1141 gatgggacaa actatgccgg accagtcatc ggaaccatta acaacctgac gacaaagtgg
     1201 atcggcgtct cgctgcgcaa tacctcggcg gattacgtct atgttctcgg caccgatgtc
     1261 atcaagagct ggcttaccca actcggtttt acgattccca caggattcaa cgtcacggcg
     1321 gtatcggtat ctgatcaatg gtatttgaag ggtggcatcg tcttcgccta caacaactca
     1381 aacggtattg cgtaccagtt cacacgcgcc gacaatgctg atggaacagc atctggaaca
     1441 agttacttta caagcgtcaa tctgggtagc ggaatcgatg acatcaagg
//
LOCUS       DAORHD010001355         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117216_length_1489_cov_2.470014, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001355 DAORHD010000000
VERSION     DAORHD010001355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117216_length_1489_cov_2.470014"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKO06_21600"
     CDS             <1..845
                     /locus_tag="PKO06_21600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72318.1"
                     /translation="AFIRARRDALRLILDRYVSGDAVFEPLYAHFVERHQQGWRPVLA
                     GLFMGAFFLIFRWSISWDLLPYRTGGTRLFITLMGALACGLFVRVLWQLLGFGMLVSG
                     MSRELEERGARLFSLELLERAGAGYARTAFGASFLSLGLFWLAMASRSLIMDRPGALA
                     ETLPSVFLVLLLALIVPLAYLILPIWRLHRIMAQRKNDIRRLFAEEHAATERRFLEQP
                     NKEMAQAYLQGRQVIEEINNLPEWPFRFETLAKLVTLVAVPAALFIFKEIVVDVAVEL
                     LKHK"
     gene            complement(862..>1489)
                     /locus_tag="PKO06_21605"
     CDS             complement(862..>1489)
                     /locus_tag="PKO06_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72319.1"
                     /translation="QRLKKGVSEEVWLNEAMSGYAEHVCGFKISTNNESKCGQVKDYL
                     EQTRIVPLVQTQGWGNTSPYINAQYGQAYLFGTWLAQHYGTLVGNRYSVASLLSSTSV
                     GGAAVTNFCGEGYDMVFARFITALYVNDASNGSKYGWKDIDLKGDYPGGPLSGVKSIT
                     EPNVTQGFSSGNITLVPYSSAYIVLSGGNGNTLSINIPTTLTVLELTK"
BASE COUNT          305 a          443 c          385 g          356 t
ORIGIN      
        1 ccgccttcat tcgcgcccgc cgcgatgccc tgcgactgat tctcgatcgc tacgtgtcag
       61 gtgatgccgt ttttgagccg ttgtatgccc attttgtcga acgacatcag caggggtggc
      121 gcccggttct ggcgggtctc ttcatgggag cgttctttct gatctttcgc tggtccatca
      181 gctgggacct tctgccgtat cgtaccggcg gcaccagact cttcatcacc ctgatgggtg
      241 cgcttgcctg cgggttgttt gtccgagttc tctggcagct actcggcttt ggcatgctgg
      301 tcagcggaat gagtcgcgaa ttggaagagc gcggcgcacg gttgttctcg ctcgagctgc
      361 tcgagcgcgc cggggcgggt tatgcccgca ccgcgttcgg cgcgtcattt ctttctctgg
      421 gtctgttctg gctggccatg gcgtcgcgca gcctgatcat ggatcgtcca ggagctctgg
      481 cagagactct tccctcggtc tttctcgtac tattactagc actgatcgta ccactggcgt
      541 atctgatcct gccgatctgg cgtttgcacc gcatcatggc gcaacgcaag aatgacattc
      601 gtcgcctgtt cgccgaagag cacgcggcaa ccgagcgccg ctttctcgag caaccgaaca
      661 aagagatggc gcaggcctat ctgcagggtc gtcaggtcat cgaagaaatc aacaacctgc
      721 ccgagtggcc gtttcggttc gaaacactgg ccaaactcgt caccctcgtc gccgtccccg
      781 ccgcgctctt catcttcaag gagatcgtcg tcgacgtcgc cgtcgaactc ctcaagcaca
      841 agtagcaggt gggccgaacc gctacttcgt cagctcaagc accgtcaaag ttgtcgggat
      901 attgatactg agcgtattcc cattcccacc ggataggaca atatacgcag acgagtaggg
      961 cacaagcgtg atgttgccag aagaaaatcc ctgagtgaca ttcggttccg tgatactttt
     1021 cacaccgctg agaggaccgc caggatagtc gcccttcaaa tcaatatctt tccaaccgta
     1081 tttcgatcca ttggaagcgt cgtttacata cagcgcagtg atgaaacgcg caaacaccat
     1141 gtcatagcct tctccacaga agttcgtcac ggcagcacca ccgacagacg tgctggacag
     1201 aaggctggcg acagaatacc gattgccaac aagggtgcca taatgctgtg ccagccaggt
     1261 cccaaacagg tatgcctgcc catactgcgc gttaatatat ggagacgtat taccccagcc
     1321 ttgtgtctgg acaagcggaa caatgcgtgt ttgctccaga tagtctttta cctgtccaca
     1381 cttcgactca ttattcgtac tgatcttgaa accgcaaaca tgctcggcat agccagacat
     1441 tgcctcattg agccatacct cttcactcac acctttcttc aagcgctga
//
LOCUS       DAORHD010001356         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117281_length_1488_cov_3.995115, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001356 DAORHD010000000
VERSION     DAORHD010001356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117281_length_1488_cov_3.995115"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(263..607)
                     /locus_tag="PKO06_21610"
     CDS             complement(263..607)
                     /locus_tag="PKO06_21610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV72320.1"
                     /translation="MELKIDVKPRGDGVTIVVLRGEIGTETVNQFKDRIDQLVAEGQT
                     KLVMDFQEVNYLNSMGLGVVAATLKKVKKSKGDLKLIRLSPAVQELFELTRLTKVFEI
                     FESEEEALKSFA"
     gene            complement(725..>1488)
                     /locus_tag="PKO06_21615"
     CDS             complement(725..>1488)
                     /locus_tag="PKO06_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72321.1"
                     /translation="MHANLTLDRKYNFFNYAHTLKNSKHHEVVALSHVRFGVDGIPET
                     REALFLAGTDPNGPNQAAGMPATDRDMSYTPGRNVYIFSYFDDTETATFGTYARKIGK
                     QWFGGVNLKSLRQDLFTNSADSWGLDLGFLYTPNQKSAIGLSIRDIAEQLKWDTASGH
                     SDTVPVTTTVGASYKTRPNMTVAVDVSKTQDLDAKFRVGTEYWFKDIAALRLGSQAGD
                     LTLGASFKLQDWRFDYAYADETLGEAHRISASKQF"
BASE COUNT          325 a          442 c          376 g          345 t
ORIGIN      
        1 tggtaacatt cactcccacg acccctgatt tttccaggcc gttcatcacg gttgcgagat
       61 cgtctcccgc caccggaaaa ggtacgtaga ccgcgtccaa tcccacttcc cgaaaggccg
      121 cgttccaaaa tcgcggactg agggaatgat gcaaagtgcg accgagaatg ccgaaaaccc
      181 gggtcgttcc ccggatctct gcaacagaga ccggacaacg gcccgggttc ttgcgacgtg
      241 tgttcaccct cgtgggaggg gctcaggcaa aactcttgag cgcttcctct tccgattcga
      301 aaatctcaaa cactttggtc agacgagtaa gctcgaacag ctcttgaaca gccggcgaga
      361 gacggatcag cttgagatcg cccttgctct tcttcacctt cttcagcgtt gcagcgacaa
      421 caccaaggcc catactgttc aggtaattca cttcctggaa gtccatcacc agtttggtct
      481 ggccttcggc caccagctga tcgatgcggt ctttaaactg gttaaccgtt tcggtaccaa
      541 tttcaccacg cagcacaacg atcgtgaccc catctcctcg cggtttgaca tcaattttca
      601 gttccataca tcctccacac caattctcga atacggtgag tgaagtctac tttactccaa
      661 actcacagga gatgcaacct gctcgtaaaa aacgccggga acgtctgtcc cggcgttttc
      721 acgttcagaa ctgtttcgag gcagagattc gatgagcctc gcccagcgtc tcatccgcat
      781 acgcgtaatc gaaacgccag tcctgcagct tgaaactggc acccagggtc aggtcgcccg
      841 cctggctgcc cagacgtaac gccgcgatat ccttgaacca gtattcggta ccgacgcgga
      901 acttggcatc gagatcctgg gtcttgctga catcgacggc gaccgtcatg ttcggacggg
      961 tcttgtacga tgccccgacg gtggtcgtga ccggcaccgt atcactgtga ccagaggccg
     1021 tatcccattt cagctgctcg gcgatgtcgc gaatcgacag accgatagca gacttctggt
     1081 tcggcgtata caggaagccg agatcgagac cccagctgtc agccgagttc gtgaacagat
     1141 cctgccgcag tgacttgagg ttgacaccgc cgaaccattg cttgccgatc ttgcgggcat
     1201 aggtgccgaa cgtcgccgtt tccgtatcat caaagtagct gaagatgtag acgttccttc
     1261 ctggcgtgta actcatgtct ctgtctgtag ccggcatacc tgctgcctgg tttgggccat
     1321 tcggatccgt tcctgctaag aacaatgctt ccctggtttc cggaatccca tcgacaccga
     1381 accgcacatg cgacagcgcc acgacttcgt ggtgtttgct gttcttcaac gtgtgcgcgt
     1441 agttgaagaa attgtacttg cggtcaagtg tcagattggc atgcatga
//
LOCUS       DAORHD010001357         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117297_length_1488_cov_3.397069, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001357 DAORHD010000000
VERSION     DAORHD010001357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117297_length_1488_cov_3.397069"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="PKO06_21620"
     CDS             <1..1031
                     /locus_tag="PKO06_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72322.1"
                     /translation="PATGKAVSPKSSAPAGADAGTPPASATPAQIQDKPTSPPVKTTV
                     PAAAKPSAGAPSAVSAAGTDGPQPAAPAPQSAPPPASQTGPVAENASAITVPKTKLSE
                     TILIRYPSFLTQPLSRLYQPASVEDHLKSLSAVIDNLVAYFLLCFLQSYLFFADRKPK
                     VDQAVKETLKIHLRGPDALRYLNILALALREQERDSFFSFVLAKVFTEPSDQNPMFAL
                     KDLVQFLQEPPPDAAEQLNGAVDTCVNALAGVKTILSNKLVFKTPPGGREPFFDLTGP
                     TPGPLASGSRPTLDLPNGEIIVLSRDRSEALGLFPYFRFDGKQVEFGQPSPQDFTMLL
                     ERLELRLD"
     gene            1024..>1488
                     /locus_tag="PKO06_21625"
     CDS             1024..>1488
                     /locus_tag="PKO06_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72323.1"
                     /translation="MTEISLSRSRPARYPRYAAVAALFLSLAFCPAFGLDPRLARFLP
                     ATSPATPTPVLSVPAIPAFSPQSVSVEAHNQGVDLNNQGLDRLRDNEVAQAIKLLYQA
                     TESDPTEKGFWNNLLVALKRDPRRGQEALAVSYKLIALEPKSAKGYQAAGSVL"
BASE COUNT          293 a          513 c          363 g          319 t
ORIGIN      
        1 ccccagcaac cggcaaagcg gtttccccga aatcgagcgc acccgccggt gctgacgctg
       61 gcactcctcc agcttcggcc acccccgctc agattcagga caagccgacg agcccccctg
      121 tcaagacgac cgttcccgcc gccgcgaaac cttcagcagg agcgccgtct gcggtttctg
      181 ccgctggcac agacgggccg caacctgctg cccccgcgcc acagtcggca ccaccgccgg
      241 cttcccagac aggcccggtc gccgagaacg cttcagccat tacggttccc aagaccaaac
      301 tcagtgaaac aattctgatc cggtatcctt cgtttcttac ccaaccgctt tcccgtttgt
      361 accagccagc ctcggtcgaa gatcatctca agagcctgag tgcggttatc gacaatctgg
      421 ttgcctactt tctcctctgt ttcctgcaat cctatctgtt tttcgccgat cggaaaccca
      481 aagtcgacca ggcggtcaaa gaaacgctca aaattcatct tcgcggtccc gatgccctga
      541 gatacctcaa catcctcgct ctcgcccttc gcgagcagga acgggacagc ttcttctcgt
      601 tcgttctcgc caaagtcttt acagaaccca gtgatcagaa cccgatgttt gccttgaagg
      661 atctggtaca atttcttcag gagccgcccc ccgatgccgc cgagcaactg aatggggccg
      721 tcgacacctg cgtgaatgcc ctcgccggcg tcaaaaccat tctcagcaac aaacttgtct
      781 tcaaaacgcc gccgggcggc cgcgaaccct tcttcgatct cacgggcccg acccccgggc
      841 cactggcgtc tggttcccgc ccgacactcg acctgccgaa cggtgaaatc atcgtgctgt
      901 cacgagaccg gtctgaggct ctcggactct ttccctattt ccgttttgac gggaaacaag
      961 tagaattcgg acaaccctca cctcaggatt tcaccatgct gctcgagcgc ctggaacttc
     1021 gccttgactg agatttctct ttctcgcagt cgaccggcgc gatacccccg ttacgccgca
     1081 gtggccgctc tttttctgtc gctggctttc tgtccggctt tcgggctcga cccccgcctg
     1141 gccagatttc ttccggcgac ttcgcccgct acgccgaccc ccgtcctttc cgttccggcc
     1201 attccggcgt tttcgcctca gtccgtctct gtcgaagctc acaatcaggg tgtcgatctg
     1261 aacaaccagg gtcttgaccg tttgcgcgat aacgaagtcg ctcaggccat caaactgctg
     1321 tatcaagcca ccgagagcga tccaaccgaa aagggattct ggaacaatct gctggtagcg
     1381 ctgaaacgcg acccgcgccg ggggcaggaa gcattggcgg tatcgtacaa gctgattgca
     1441 cttgaaccca agtcagcaaa agggtatcaa gctgccggat cggttctg
//
LOCUS       DAORHD010001358         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117444_length_1487_cov_3.963687, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001358 DAORHD010000000
VERSION     DAORHD010001358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117444_length_1487_cov_3.963687"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..984
                     /locus_tag="PKO06_21630"
     CDS             <1..984
                     /locus_tag="PKO06_21630"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNV72324.1"
                     /translation="QVRRAIMRNHTATHLLHKALQETLGTHVKQAGSLVSADRLRFDF
                     THFEGVSREKLLEIEKKVNAVILEALPVNTIATSYDDAVKRGAMALFGEKYGDRVRMV
                     EVGGYSRELCGGTHLKNSAEAGFFTILSEASVAAGVRRIEAVTGEGAFQRFEAVRSEL
                     QHLGSLLETDPKNLPDKINRLQQEMDALKQEIQSMRKKELKEKLQQARTQTRTLGMLK
                     TIILRTDGLSVDEMKEVADQLVEGQAGTVVLLAAASDKANFVLKGTKDLVEAGFHAGK
                     LVKEIAKEAGGSGGGKPELATAGGKEPEKIDQALTVAEKLFTGWTKGTTAR"
     gene            1074..>1487
                     /locus_tag="PKO06_21635"
     CDS             1074..>1487
                     /locus_tag="PKO06_21635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72325.1"
                     /translation="MARSEKNKELSGQENVELKQEVENTTPDASRDVEPAVEEAGFLD
                     WLIDNNPLYLLSVIFMFGGLLLVSKASYQVPGQAGPDASLSTVVAFFGVQNVYEIIMV
                     GMAIYLIANKVNARHGKILLALELLFLADLTYYQVR"
BASE COUNT          354 a          377 c          459 g          297 t
ORIGIN      
        1 caggtccgca gggcgatcat gcgcaatcac acggcaaccc atttgctgca caaagctctg
       61 caggaaacac ttggcacgca tgtgaaacag gccggatcgc tggtttccgc tgaccgactg
      121 cgattcgatt tcacacattt cgagggtgtt tcacgggaga agcttctcga gattgagaag
      181 aaagtcaacg cggtgattct cgaagctctg ccggtgaaca cgattgcgac gtcgtatgat
      241 gatgccgtca agcgtggcgc catggcgttg ttcggcgaaa aatacggaga tcgggtgcgt
      301 atggttgagg ttgggggcta ctctcgcgaa ctctgcggtg gtacgcatct gaagaactcc
      361 gctgaggccg gattctttac gattctcagc gaagcctcgg ttgcggcggg agtgcgtcgc
      421 atcgaggccg tcacggggga aggggcattt cagcggtttg aagcggtgcg gtcagaactg
      481 cagcatctgg gttcgctgct cgaaaccgac ccgaaaaatc ttcctgacaa gatcaaccga
      541 ttgcagcagg aaatggacgc cctgaaacag gaaattcagt ccatgcggaa aaaggaactg
      601 aaggaaaaat tgcagcaggc ccgcacccag acacgtactc tcggaatgct caaaacgatc
      661 atcctgcgca cggatggact ctcggtcgat gaaatgaagg aagtcgctga tcagttggtc
      721 gaaggccagg ctggcaccgt ggtgttactc gccgcggcct cggacaaggc gaactttgtg
      781 ctcaaaggca cgaaagatct cgttgaggcc ggcttccatg ccggaaaact ggtcaaggaa
      841 atcgccaaag aagcgggtgg cagtggtggt ggcaagccgg aactggcaac ggccgggggt
      901 aaagaaccgg agaagattga ccaggctctg acggtcgccg agaaactgtt caccggttgg
      961 accaaaggaa ctaccgcccg atgagggggc ggacacgttt ggcctgaaac caaggtgcgg
     1021 ctctgaagat cagggccgca ccgctgtttt accgcaaaca aggggggcac tggatggcac
     1081 gctcagaaaa gaacaaggaa ctttcggggc aagagaatgt tgaattgaag caggaagtgg
     1141 aaaataccac cccggacgct tcgcgtgacg ttgaaccggc agtggaagaa gcgggttttc
     1201 tcgactggct gatcgacaac aatcccctgt atctgctgag cgtgattttc atgttcggcg
     1261 gcttgctgct ggtgagcaag gcgagttacc aggttcccgg gcaggctggt cctgatgcct
     1321 ctctctcgac ggtcgtcgct tttttcggcg tccagaacgt ctacgagatc atcatggtgg
     1381 gaatggccat ctatctgatc gccaacaagg tcaatgcccg acacggcaag attctgctgg
     1441 cgctcgaact gctctttctc gccgatctga cctactatca ggtgcgc
//
LOCUS       DAORHD010001359         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117449_length_1487_cov_3.796788, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001359 DAORHD010000000
VERSION     DAORHD010001359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117449_length_1487_cov_3.796788"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..366)
                     /locus_tag="PKO06_21640"
     CDS             complement(169..366)
                     /locus_tag="PKO06_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72326.1"
                     /translation="MSPMMPNRKLSEDDVVKLRSEKTALNRHSRRMTGMTQVCIGKVE
                     GQLECETTEGQCPDYSECHKK"
     gene            complement(563..1234)
                     /locus_tag="PKO06_21645"
     CDS             complement(563..1234)
                     /locus_tag="PKO06_21645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72327.1"
                     /translation="MKTLAIVLFGILFLLPGMAGAANVKADILAGLEEYGAKVKDLET
                     RFQELKKSGALYEPPEGGNRIHGTQWDVLLQEAERAVIHANGYFDRATRTGDASFDDP
                     KVKAMLAEFEAYKERLRKSIDWFSPLQYAEKPIPELTDLLDAVRNMRRAYVNAKAGVS
                     PEETQESVRVAFYYFDQMLEKNKAGGPDATPDLEKHPHFIAAKERMTFYQKEIDSLSA
                     PSTSK"
     gene            complement(1259..>1487)
                     /locus_tag="PKO06_21650"
     CDS             complement(1259..>1487)
                     /locus_tag="PKO06_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72328.1"
                     /translation="LARKNPALEVSVPDLLAEAEKTLADLVRAVKVADAGDPKVTPDL
                     EKHPAFLTAKTEVDRLVTEAAKLLAKSAAPK"
BASE COUNT          300 a          428 c          406 g          353 t
ORIGIN      
        1 tgcagcccct ggaccgacga cttcgtgaac ccctgggtcc ccctgttgtc cttcgatctt
       61 ctcgatgtcg tcactccgcc tgttgccgaa aatcgagacc ctgcctatga acaggcaggg
      121 tctcgataag agccgcaggt atcgcgcggc agaagtcaac tgaaccgctt atttcttatg
      181 acactcgctg tagtcggggc actgtccttc ggtggtttcg cactcgagct gtccttcgac
      241 cttgccgatg cagacctgcg tcatgccggt catacggcgg gaatgacgat tcagagcggt
      301 tttttctgag cgcagcttga cgacatcgtc ttcagacaat tttcggttag gcatcatcgg
      361 actcatatct cacactcctt ctgagcagta tgcggaaagt gccgtcacta tacccgaacc
      421 gcccgcaacc ggcaagcact gctggttcaa accacagggc aaacgggaac gggtcgatga
      481 cacagagggc atgcgcgctc ccgcgaagac agagcacgaa agcgaaccca agttcatccg
      541 gaccatgaat cgatgatgtc gcttacttgc tggttgaagg cgcgctcagc gagtcgatct
      601 ctttctgata gaaggtcatt cgttccttcg cggcaatgaa atgcgggtgt ttttccagat
      661 cgggagtcgc atccgggcca ccggccttgt tcttttcgag catctgatcg aagtagtaga
      721 aagccacccg aacgctttcc tgcgtctctt cgggggaaac cccggctttg gcgttgacgt
      781 aggcgcgtcg catgtttctg accgcgtcga ggagatccgt gagctcggga atcggtttct
      841 cggcgtactg caacggagaa aaccagtcga tgctctttcg caaacgctct ttgtatgcct
      901 caaactccgc aagcatggct ttcacctttg ggtcgtcgaa actggcatca ccggttcgag
      961 ttgcccgatc aaaatacccg ttggcgtgaa tgaccgcccg ttcggcttct tgaagaagta
     1021 catcccactg cgtgccatga atacggtttc caccctccgg tggctcgtac aaagccccgg
     1081 atttttttag ttcctgaaag cgggtttcca agtccttgac cttggctcca tactcctcga
     1141 ggccggcaag aatgtcggcc ttgacattcg ctgcaccagc cattccagga aggagaaaga
     1201 ggattccgaa cagaacgatg gcaagcgttt tcataaaggg tcgctccttt gcgtgcttct
     1261 atttcggggc tgcgctcttg gcaagcagct tggctgcttc cgttaccaga cgatcgacct
     1321 cggttttggc cgtcaggaag gcagggtgtt tttccagatc gggggtcacc ttcggatcgc
     1381 cggcatcagc aaccttcacg gcccgcacga gatcggcgag cgtcttttcg gcctcagcca
     1441 ggagatccgg cacggatact tcaagcgccg ggttctttcg ggcaagg
//
LOCUS       DAORHD010001360         1487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117469_length_1487_cov_3.141061, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001360 DAORHD010000000
VERSION     DAORHD010001360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117469_length_1487_cov_3.141061"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..>1487
                     /gene="murC"
                     /locus_tag="PKO06_21655"
     CDS             514..>1487
                     /gene="murC"
                     /locus_tag="PKO06_21655"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNV72329.1"
                     /translation="MNHRQGVHFIGIGGVGMSGLARILVEGGTPVSGSDLGPNAQTAL
                     LSSFGARIFYKQCAGNIPENTGKVVVSTAIDHKNPELLEARARGIPIVHRSDLLAEMF
                     LDAHNGIAVAGCHGKTTVSSMISHVLVHGDVDPTCVVGGTVAALGGNARTGKSGVFVA
                     EADESDATFLKYFPQVAIITNIDNDHMDHYQSLDAIVKAFEVFASHVQPNGTLYVCQD
                     DPIARNIALPEQRRIVSYGIDTPADIMASNVQLFPFGSSFDVTIAGIKRGRFTLSIPG
                     KHNVLNALPVISIGFDLGLDADKIAKSLKTFSGAGRRFEVKGNYDGITV"
BASE COUNT          318 a          417 c          422 g          330 t
ORIGIN      
        1 tttttgctca gcgaggcata gtcgccgaga ccggcaatct gcgagccttt gatgagaatg
       61 gaggtatcgg tcagcacatc agcattgcca ccgggccaga catacgacgc attcttgata
      121 atcagattca tgtcgttctc catcatggaa gtgaaatacc gcgtcagagt agcacgcccg
      181 gctttttctc gtaaagcacg ctcattccgg catcagggtc atttgattct cccctgtcct
      241 tcatcgagcg atgcgaatgg aaacacggtg ttctgaaact tattgaatga cccacagagt
      301 gagatcgttt ggttgcacag ggggaagagg tgtggtatgt tacccggcgt tttaccgaat
      361 ggcttttgac acaatcgttc gtttacaacg tgctttggct ggttctgacc tgcccggaaa
      421 aattgttctc aggtttcggg agatatccgt cgatggttta cgagaaacag aaacgtccgt
      481 gaccaggcca agcaaggagg cagatacagc accatgaacc atcgacaggg agtccacttc
      541 atcggcatcg gcggcgtcgg tatgagcggc ctggcccgca ttctcgtcga gggcggcacg
      601 ccggtttccg gctccgatct cggcccgaac gcacaaaccg ccctgctgag cagcttcggc
      661 gctcgcatct tctacaagca gtgcgccggc aatatccctg aaaacactgg caaggtcgtg
      721 gtttcgaccg ccattgatca caagaatcct gagctgttgg aagcacgggc acgcggcatt
      781 cccattgtgc accgctctga tctgctggcc gaaatgtttc tcgatgccca caacggcatc
      841 gccgtcgccg gttgtcacgg caaaacgacg gtcagttcga tgatttcgca tgtgctcgtg
      901 cacggtgatg tcgacccgac ctgcgtggtc ggcggcacag tcgcggcact cggtggaaac
      961 gcccgcaccg ggaaatccgg tgtgttcgtg gccgaagccg acgaaagcga cgcgaccttc
     1021 ctgaagtatt ttccacaggt tgcgatcatc acaaacattg ataacgacca catggatcac
     1081 taccagagcc tcgatgcgat cgtgaaagcg ttcgaggtgt ttgccagcca tgtgcagccc
     1141 aacggtaccc tgtatgtctg ccaggacgac ccgatcgcgc gcaacatcgc gttgccggaa
     1201 cagcgtcgca ttgtgtccta cggcattgat acgccggccg acatcatggc ttcgaacgtg
     1261 cagctgtttc catttggttc atcgttcgac gtgacgatcg cagggatcaa acgcggtcgg
     1321 ttcacgctgt cgattccggg caagcacaat gtgctgaatg cgttgccggt catctcgatc
     1381 ggcttcgatc tgggcctcga cgccgacaag atcgcgaagt cgctgaagac gttctcgggt
     1441 gcggggcgcc gcttcgaggt gaagggcaac tacgacggta tcaccgt
//
LOCUS       DAORHD010001361         1486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117624_length_1486_cov_2.533892, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001361 DAORHD010000000
VERSION     DAORHD010001361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117624_length_1486_cov_2.533892"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1486)
                     /locus_tag="PKO06_21660"
     CDS             complement(<1..>1486)
                     /locus_tag="PKO06_21660"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV72330.1"
                     /translation="EIVALPEGVFLKKPLERQDTGCLLLAIPMPFPLNQPRAFRWGLE
                     GLFLLMFAWLARHLTLILLGRRRVVIDLAVQIALFFVSAGLIPLFTLAFLGASRALET
                     EAIERVRWEKEMIDLLVRADHQFKNWTENLQITCLDLARKLTLDRVTNLSPDLIPAEL
                     KRANSVIFVLAREVDRASTMYSSLRPDLERDLGNQAASLQKVLLDLFRQMLIERGERQ
                     TSASGEKPKMVLTVQDILGEGSPVLEIVKHLGVMTTSEFNESLLLSFCHLLENRQGEA
                     QSFMMVMMDLALEVRQAFPRIKGMLAQRDSPLRLTTIYELSETAAEREFDVSFIRETV
                     QRVQSEQSQETARITSLRGEDFLFSARHMSFLQDFMPCCLVRYSVITYGKRLQVVVIV
                     TMFLLGIGQIILVSSILRRAILVPVAYLRKGVEAVAGGDYQHRIPIEAANELGDLSGA
                     FNDMISGLQERERMRRFVSASTVEDIRRTRDGQGGDGNGRLVTVA"
BASE COUNT          363 a          407 c          426 g          290 t
ORIGIN      
        1 ggcgacggtg acgagacggc cgttcccatc gccgccctga ccgtcacgag tgcgcctgat
       61 atcctcaacc gtcgatgcgg aaacgaagcg tcgcatgcgc tcccgttcct gcaatccgct
      121 gatcatgtcg ttgaaagcac ccgagagatc gccgagctca ttcgcggctt caatgggaat
      181 acgatgttgg taatccccgc cggcgacggc ttcgaccccc tttctcaggt aagctactgg
      241 taccagaatg gcccggcgca ggatggatga aaccagaatg atctgaccga tgccgagaag
      301 gaacatggtg acaatcacca cgacttgaag gcgtttgcca taggtaatga ccgagtagcg
      361 tacgagacag catggcatga aatcttgaag aaaactcatg tgacgggcag agaaaaggaa
      421 atcctcaccc cgtaacgagg tgattcgagc agtctcctgg ctttgctcag actggacccg
      481 ttgaacggtc tcgcgaatga aggatacgtc aaattccctt tcagccgcgg tttccgacaa
      541 ttcatagatc gtcgtcagcc gaagcggcga gtctctctgc gccagcattc ccttgatccg
      601 gggaaatgcc tgtcgcactt ccagagccag atccatcatg accatcatga agctctgggc
      661 ttctccctgg cgattttcga gcagatgaca gaaagaaaga agcagactct cgttgaattc
      721 tgacgtggtc ataactccaa ggtgcttgac gatctccagg acgggggaac cttcgccaag
      781 gatatcctgc accgtcagga ccattttggg cttttctccg gatgcggacg tctgacgctc
      841 tccgcgttcg atcagcatct gtcggaaaag gtcgagcaga accttctgca gggatgcggc
      901 ctgatttccc agatcccgct ccagatcggg gcgaaggctc gaatacatgg tcgaagcacg
      961 atcgacctcc cgggcgagga caaagatcac ggaattggcc cgtttcagtt ccgcggggat
     1021 gaggtcggga gaaaggttcg tcacccgatc gagcgtgagc ttacgggcaa gatcgagaca
     1081 ggtgatctga aggttctccg tccagttctt gaattggtga tccgccctga ccagaaggtc
     1141 gatcatttcc ttttcccagc ggactctttc gatggcctcg gtttcgagag cacgcgaagc
     1201 cccgagaaag gccagggtaa acaggggaat gaggccggcg gaaacaaaga acaacgcgat
     1261 ctgtacggca aggtcgatga cgacacgtcg tcgcccgaga agaatcaagg tcaggtggcg
     1321 cgcgagccac gcaaacatga gaagaaacag gccttcgaga ccccagcgaa aagcccgtgg
     1381 ctgattgagc gggaacggca tgggaatggc caacagaaga caacctgtat cctggcgttc
     1441 caagggtttc ttgaggaaga ccccttcggg cagagcaaca atctcc
//
LOCUS       DAORHD010001362         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117834_length_1484_cov_3.847446, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001362 DAORHD010000000
VERSION     DAORHD010001362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117834_length_1484_cov_3.847446"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1014
                     /gene="atpG"
                     /locus_tag="PKO06_21665"
     CDS             142..1014
                     /gene="atpG"
                     /locus_tag="PKO06_21665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNV72331.1"
                     /translation="MPNPRDIRKKISGVKNTQQITRAMKMVSTAKLRKAMERLNSTKF
                     YLTKLEDLMSDLQGVLGDRSVLQQYAARPVNCAGIVVVTGDRGLCGSFNSDIIKAALR
                     HIQQLPAQEKRLITFGRKGYDFFRRRQWPIISSYSEMNGNLDSDGIRAAARDAVALFL
                     QDCFDELWIVFADFVTVLERKVIIKRLLPLDPITTTRKRQHVLTHELMFDPSPEVIFD
                     ALIPRFVEGLFLRAVLESATSEQAARMIAMGAATDKADEIISELTLFFNRTRQAIITK
                     ELSEVVAGADALSG"
     gene            1054..>1484
                     /locus_tag="PKO06_21670"
     CDS             1054..>1484
                     /locus_tag="PKO06_21670"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011248685.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNV72332.1"
                     /translation="MNEGHITQVIGPAVDVKFPPESLPNIYNALEVENPATKLKLVLE
                     VAVHLGNDVVRAVALGPSEGLIRGLQVKDTGGPISVPVGPRVLGRLFNLLGETLDGRE
                     PVAETERWPIHRPAPQLAAQGKTTEIFETGLKVIDLLLPYPR"
BASE COUNT          323 a          474 c          414 g          273 t
ORIGIN      
        1 tcgatccgca gctcaaggtc cttacccgcg atcacatcaa cagcctgctg aaggaattcg
       61 tcgaactgta caaggcagaa acgaagcaac ttcccttgaa caaggcaact ccggcggaag
      121 aaaaggcggt ttcctgagcc catgccgaat ccgcgtgaca tacgcaaaaa gatctcgggg
      181 gtgaagaaca cccagcagat cacgcgcgcc atgaagatgg tctcgaccgc caaactgcgc
      241 aaggcgatgg aacgcctcaa ctcgacgaaa ttctatctga ccaagctcga ggatctgatg
      301 tccgatctgc agggcgttct cggtgaccgc agcgtgctgc agcagtatgc ggcacgaccg
      361 gtcaactgcg cgggtatcgt cgtcgtcacc ggcgaccgcg ggctctgcgg cagcttcaac
      421 tccgatatca tcaaagccgc gttgcgccac attcagcagt tgccggctca agagaagcgc
      481 ttgatcacgt tcggacgcaa ggggtacgac ttctttcgcc gccggcagtg gccgatcatc
      541 agttcatact cggagatgaa cggaaacctc gattctgacg gcatcagggc ggccgcgcgc
      601 gacgccgtgg cgttgttcct gcaggactgc ttcgacgaac tctggatcgt tttcgccgac
      661 ttcgtgaccg tgctcgaacg caaggtcatc atcaagcggc tgctgccact cgacccgatc
      721 accacaaccc gcaaacgaca gcacgtactc acgcatgagc tgatgttcga cccttcgccc
      781 gaggtcatct tcgacgcact gattccgcgc ttcgtcgagg ggctgttcct gcgcgccgtt
      841 ctcgaatcag ccacttcaga acaggcggca cgcatgatag ccatgggcgc agccactgac
      901 aaagccgacg agatcatcag cgaactgact ctgttcttca accgtacccg ccaggccatc
      961 atcaccaagg aactcagcga ggtggtggcg ggtgccgacg ctctttccgg ctgaagtccg
     1021 gaatcgcacc acgatagccg caggagactg cttatgaatg aaggtcatat cacccaggtc
     1081 atcggaccgg ccgtcgacgt taagttcccg cccgagagcc tgccgaacat ctacaacgcc
     1141 ctcgaagtcg agaacccagc cacgaaactg aaactcgtgc tggaagtcgc cgtgcatctt
     1201 ggcaacgacg tggtgcgcgc cgtcgcgctc gggccatcgg aaggtctgat tcgcggcctg
     1261 caggtaaaag acaccggtgg cccgatttcc gtaccagttg gtcctcgcgt gctcgggcgt
     1321 ctgttcaatc ttctgggtga aacgctcgac ggtcgcgagc cggtcgccga aactgagcgc
     1381 tggccgattc atcgcccggc ccctcagctg gccgcgcagg gcaaaaccac cgagatcttc
     1441 gaaaccggtc tcaaggtcat cgacctgctg ctcccgtacc ctcg
//
LOCUS       DAORHD010001363         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117869_length_1484_cov_2.937719, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001363 DAORHD010000000
VERSION     DAORHD010001363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117869_length_1484_cov_2.937719"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKO06_21675"
     CDS             <1..1078
                     /locus_tag="PKO06_21675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72333.1"
                     /translation="LCLKALQNDLSQLREFQAADPRTFRSERRHLFEVSPIRLSFATT
                     RAELVTYAFEPTAWSLTRTVAPLDERELSVRELIMKKVRERVEKTFAAVTDEVRAQVQ
                     EKVNELQEAFTLGDPRQALEDPVALTQRCTVVFDKLQYYTGTGNGEPPFVADLKTLVT
                     TRAQKMAADIGYGIEADAEEIMGTVLTRLFQLEELAPPRLNRVTTSSRTFRLKGSYRL
                     VFADGTEPAPSSAALSDGRLRGFRFFLESTNPVSALEVFYQKQGVLIAPNALAQESTA
                     TLAHWNQNPNPRVKLKVMDLITTQELTGRYFGEAIIPDANILYKTWVEIPEFWFLDIF
                     NTEAEVDKLRKGLINPDDAEPKQL"
     gene            1149..>1484
                     /locus_tag="PKO06_21680"
     CDS             1149..>1484
                     /locus_tag="PKO06_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72334.1"
                     /translation="MIEVTLTILLFALLIPALFDLQSGSERAVVKAGEFMVASALLQN
                     TSARYRTIPFGDIEPLTENHDETGTLISTGRPAFQVNVQVESVVMPNRWSKYKRVNIE
                     VLKPGTFFGT"
BASE COUNT          335 a          451 c          407 g          291 t
ORIGIN      
        1 tctctgtctc aaagcacttc aaaacgacct ctcccagctt cgcgagtttc aggcggctga
       61 cccccgaacc tttcgttccg aacgcagaca cctcttcgag gtctcgccga tcagactgtc
      121 attcgcgacc accagggcgg aactcgtgac ctatgcgttt gagccgacgg cgtggtctct
      181 gacacgcacc gtcgctccgc tcgatgagcg tgaactgtcc gtgcgcgagc tgatcatgaa
      241 gaaggttcgt gagcgtgtcg agaaaacatt tgccgcggtc accgacgaag tcagagccca
      301 ggtgcaggaa aaggtgaatg aactgcagga ggccttcacg ctcggtgacc ctcgccaggc
      361 gctggaagac ccggtcgcgc tgacccagag atgcaccgtc gttttcgaca agcttcaata
      421 ctacacggga accggaaacg gcgagccgcc cttcgtcgcc gatctcaaga ccctcgtgac
      481 gactcgagca cagaagatgg cggccgacat cggttacggc atcgaagccg atgccgaaga
      541 gatcatggga acagttctta cccgattgtt ccagctcgaa gaactggctc cgccgcgatt
      601 gaaccgagtg accaccagct cacgcacttt ccggctcaaa ggttcgtatc gtctggtctt
      661 cgccgacggt accgagccgg cgccatcatc agcagccttg tccgatggac ggttgcgggg
      721 atttcgattc tttctggaga gcaccaaccc ggtgtccgca ctcgaggtgt tttaccagaa
      781 acagggtgtc ctgatcgccc ccaatgcgct cgcgcaggaa tcgacggcaa cgctcgccca
      841 ctggaatcag aacccgaatc cgagggtgaa actgaaggtc atggatctga tcacgactca
      901 ggaactgacc ggccggtact ttggcgaagc catcattcca gacgccaaca tcttgtacaa
      961 gacctgggtc gaaattccgg aattctggtt tctcgacatt ttcaataccg aagcggaagt
     1021 cgacaaattg cgcaaaggac tgatcaatcc ggatgacgcc gaaccgaaac agctctgaca
     1081 gtctgccacg tttctccggc ataccgatcg ccgggcaacg gcgcccgcca cgcaaacggg
     1141 gggtcggcgt gatcgaggtc acgctcacga tcctgctctt tgccctgctg ataccggctc
     1201 tgttcgatct gcagagcggc tctgagcgcg cagtggtcaa ggcaggtgaa ttcatggtcg
     1261 ccagcgcgtt gctccagaac acctcagcca ggtatcgcac gataccgttt ggcgacatcg
     1321 aacccctgac ggaaaatcat gatgagaccg gaacgctgat cagcaccggt cgcccggctt
     1381 ttcaagtcaa cgttcaggtc gagtccgtcg ttatgcccaa ccgatggtca aagtacaagc
     1441 gcgtcaacat tgaagtcctc aaaccaggca cgtttttcgg aacg
//
LOCUS       DAORHD010001364         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117973_length_1483_cov_4.273109, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001364 DAORHD010000000
VERSION     DAORHD010001364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117973_length_1483_cov_4.273109"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1483
                     /locus_tag="PKO06_21685"
     CDS             <1..>1483
                     /locus_tag="PKO06_21685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72335.1"
                     /translation="GLYRHLLAQVRVDSPGILALKSLLDPDIFTDLHRFSAVSESLES
                     SFACTLDPTGSAQLAWDSFRHPHSVDSSAAWVMLLESDLLIPSTVRSWLSDQTAEILS
                     WNPDAVGISLISDSQLIFGLTLIGLLRRAAPRLRLIVGGDAISYRRSLLSRIPQLHSF
                     ADHVVGGDAEPFWSHLLASREESVYSNQVGLSDTPSELAQRFCRCDFSVSALPAWHLL
                     PLNDYLTPSIVIPIETARGCPWGRCAFCIHPGRSPQGNPGYRLKPLDLLQRELESAIS
                     RGFHRFFFVDEALSEHRFREICALSASLSRPISWIAYARCDAPQQADFWKQVRRAGCR
                     KLFFGLETGSDRLLQQYHKGTCATTIERTLRDVAEADIAFHVFLMTGFPGETAADRQA
                     TCNLLRRVLPNVDAFGFSYDLFCLQAELETPLFLHPESYACHLDTHAPHRDLAYQYPC
                     HPLPATSELRQYADNMHSLIEASLAHRPGLRQVRLSQDSHHLLL"
BASE COUNT          256 a          483 c          360 g          384 t
ORIGIN      
        1 cggtctgtat cggcatctcc tcgctcaagt acgagttgat tctccgggta ttctggcatt
       61 gaagagcctt ctcgatccag atatctttac agaccttcat cggttctcgg cggtctccga
      121 gtcgctcgag tcgtcattcg cgtgcacgct cgatcccacc gggtctgcac aacttgcctg
      181 ggactcgttc agacaccctc actccgtcga ctcatctgcc gcgtgggtga tgcttctcga
      241 atcagacctt ctcatccctt cgaccgttcg ttcctggttg tcagatcaga ccgccgaaat
      301 cctctcctgg aacccagatg cagtcggcat cagcctgatc tctgattccc agctgatttt
      361 cgggctgacc ctgatcggtc ttctgcggcg agcggcgcct cgccttcgtc tcatcgtggg
      421 tggcgatgcc atttcttatc gacgatctct gctctcgcgc atcccccaac tccactcgtt
      481 cgcggatcat gttgtcggag gtgacgctga gccattttgg tctcatctct tagccagtcg
      541 tgaagaatct gtctactcga atcaggtagg actctcagat actccatcgg aactggcaca
      601 gcgattctgt cgttgcgact tctcggtttc tgcgctgcca gcctggcacc tgctgccttt
      661 gaacgactat ttaaccccgt ccatcgtgat ccccattgag accgcccgcg ggtgcccatg
      721 gggtcggtgc gccttctgta ttcaccctgg ccgatcgcct caagggaacc ccggctatcg
      781 actcaagccg ctcgatctgc ttcagcgcga actcgagtcc gccatttccc ggggatttca
      841 ccgttttttc tttgtcgacg aagccctctc tgagcatcgc tttcgcgaaa tctgcgcttt
      901 gtcagcgtct ctctcgcgtc ctatctcctg gatcgcctac gccagatgtg atgcgccgca
      961 acaggctgac ttctggaaac aggttcgtcg cgccggttgc cgcaagctgt ttttcggtct
     1021 cgagaccgga tcagacagac tgttacagca gtatcacaaa ggcacctgcg ccaccacaat
     1081 cgagcggaca cttcgagatg tcgctgaggc cgatatcgcg ttccatgtgt ttctcatgac
     1141 tggtttcccg ggtgagaccg cggccgaccg ccaggcaacc tgcaatcttc tccggcgagt
     1201 ccttcccaac gtcgacgcat tcggattcag ttacgatctg ttctgtctgc aggccgaact
     1261 cgaaacaccg ctgtttctgc atccggagag ctatgcctgt caccttgaca cgcacgcccc
     1321 gcatcgtgat ctggcctatc agtatccctg ccatccactg ccggcaactt cggaacttcg
     1381 tcagtatgcc gacaatatgc actcactcat cgaggcctct ctggcacatc gtcccggtct
     1441 tcgacaagta cgtctgagtc aggattctca tcatctcctg ctg
//
LOCUS       DAORHD010001365         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_117983_length_1483_cov_3.643557, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001365 DAORHD010000000
VERSION     DAORHD010001365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117983_length_1483_cov_3.643557"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKO06_21690"
     CDS             complement(<1..256)
                     /locus_tag="PKO06_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72336.1"
                     /translation="MIIRLSMTQRYKIGAAFAVFVVVFLGLTSVYQLHLLTQHEESLV
                     QDRIRGISALNSLLQIQERFQRLEIPTGYVLRPELNQELQA"
     gene            complement(253..957)
                     /locus_tag="PKO06_21695"
     CDS             complement(253..957)
                     /locus_tag="PKO06_21695"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNV72337.1"
                     /translation="MQYAVIGLGRFGSKLATTLFALGGEVLAIDTNREAVERIKDHVS
                     HVAIADCTDELALRDLGVQDMDVVIIAIGEKVETSILTTAILRRLGCPRIMSRSVSRM
                     QGQILLDVGAHDVFSLEEQMGEQMARRLAAPQILDSITLSSGHSMVDFAVPVEFVGKS
                     LKELNIRARFGINIIAVKRRIPAINERGENIVKVTLNDLPSPDEKITADDILVAVGSD
                     AKLEAMTSSFTREVAE"
     gene            complement(973..>1483)
                     /locus_tag="PKO06_21700"
     CDS             complement(973..>1483)
                     /locus_tag="PKO06_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNV72338.1"
                     /translation="TAGFNTVDMGLLSTPTIFIMIVLMFIGASPGSTGGGIKTTTFAI
                     MVLTLISQIRGESEVNLRERNVPLDIVLKAFLIAAISGGLIALYTLLLLITEAKSLSS
                     LLFEVVSAFATVGLSLNFTPQLTSWGRVIIISLMFIGRVGPLTLALSFHHPPRRGDVR
                     YPATRIMVG"
BASE COUNT          342 a          399 c          445 g          297 t
ORIGIN      
        1 gcgcctgaag ttcctggttc aactcagggc gcaggacata tccggtgggg atttcaagcc
       61 gctgaaatcg ttcctgtatc tgaagaagcg agttcagggc cgagattccc cgaatacgat
      121 cctggaccag cgattcttcg tgctgggtca gcagatgcag ctgatacacg gaggtcaaac
      181 cgagaaacac cacgacaaac acggcgaagg cggcaccgat tttgtaccgc tgggtcatcg
      241 agagacggat gatcactcgg ccacctcccg cgtgaagctc gaggtcatgg cttcgagttt
      301 cgcgtcagaa ccgaccgcga ccagaatgtc gtctgccgtg attttttcgt caggcgacgg
      361 aagatcgttc agcgtcacct tgacgatgtt ctcaccccgt tcgttgattg ccggaatccg
      421 gcgtttcacg gcaataatgt tgataccgaa acgagctcgt atattcagct ccttcagcga
      481 ctttccgacg aattcaactg gtacggcaaa gtcgaccatc gaatgaccgg agctgagggt
      541 tatgctgtcg agaatctgtg gcgctgcgag tcggcgtgcc atctgttcac ccatctgttc
      601 ctcgagggaa aagacgtcgt gagcgccgac gtcaagaaga atctgcccct gcattcggct
      661 gacgctgcgc gacatgatgc gcgggcaacc caggcggcgc aggatggcag tggtcagaat
      721 gctggtctcg actttttcac cgatggcgat gatgacgaca tccatgtcct ggacgccgag
      781 atcgcgcaga gccaactcat cggtgcaatc tgcgatggct acgtgtgaga cgtgatcctt
      841 gatgcgttcg acggcttcac ggttggtgtc gatggcgagc acttcgcctc cgagcgcaaa
      901 caatgtggtc gccagtttcg agccgaaacg gccaagaccg atgacagcat actgcatgca
      961 atgtctcctg aatcacccga ccatgattcg cgtggccgga taccggacat ccccccggcg
     1021 gggaggatga tgaaaggaca gcgccagggt caacggaccg accctgccga tgaacatgag
     1081 cgagatgatg atgactctgc cccaggatgt caactgcggg gtgaagttca gagaaaggcc
     1141 gacagtggca aaggcggaaa cgacctcgaa caggagcgat gacagggact ttgcttccgt
     1201 gatcagtaac agcagcgtgt agagtgcgat gaggccaccc gaaatcgcgg caatgagaaa
     1261 ggccttcaga acgatgtcga gcgggacgtt gcgctcgcgg agattcactt cggattcacc
     1321 tctaatctgc gagatcagcg tcagcaccat gatcgcgaag gtggtggttt tgatgccgcc
     1381 acccgtcgag cctggagaag cgccgatgaa catgaggacg atcatgatga agatcgtcgg
     1441 cgtgctcagt aaccccatgt cgacggtgtt gaatccagcg gtg
//
LOCUS       DAORHD010001366         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118006_length_1483_cov_3.047619, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001366 DAORHD010000000
VERSION     DAORHD010001366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118006_length_1483_cov_3.047619"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /gene="upp"
                     /locus_tag="PKO06_21705"
     CDS             complement(<1..365)
                     /gene="upp"
                     /locus_tag="PKO06_21705"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNV72339.1"
                     /translation="MSKVTVVEHPLVKHKLTFLRSKFTDVHMFRELMKELSMLLAYEA
                     TRDIPVEETTCETPMMKTRSHLVSGQLIALVPILRAGLGMVDGLLSFMPMAKVGHVGL
                     YRDHDTLKPVEYYCKLPNNL"
     gene            complement(429..890)
                     /gene="rpiB"
                     /locus_tag="PKO06_21710"
     CDS             complement(429..890)
                     /gene="rpiB"
                     /locus_tag="PKO06_21710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNV72340.1"
                     /translation="MRKLFLGEGVKIFIGSDHAGFGLKNKLMAYLQQAGHEVSDLGTH
                     AETSCDYADFAHPVCKGVLAQVPSFGILICGSGIGMSMAANRHHGIRAALCHNSLEAR
                     LTREHNDANVLVLGARIIGEDLAKDILDTFLRGTFAGGRHQARVAKIESCT"
     gene            1118..>1483
                     /locus_tag="PKO06_21715"
     CDS             1118..>1483
                     /locus_tag="PKO06_21715"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNV72341.1"
                     /translation="MNRVLWHGDCLQTAALMERGSVDLVYFDPPFMTGKERHGTRQEF
                     VFDDQWQGDLASFLPWLQKRIAAVIPLVSRHGSLIVHLDHRTIHYVKVWLDQHLGVKT
                     FVNEIIWHYTGGGRSRSRFS"
BASE COUNT          331 a          438 c          382 g          332 t
ORIGIN      
        1 agattgttcg gcagcttgca gtaatactcg accggcttca aggtatcgtg gtcacgatac
       61 agacccacgt gcccgacctt ggccatcggc atgaagctca acaatccgtc gaccatgccg
      121 agacccgcgc gcagaatcgg cacgagcgca atcaactgcc ccgatacgag atgacttctt
      181 gttttcatca tcggggtttc acacgtcgtt tcctcgaccg gaatatctcg cgtggcctca
      241 taggccaaca gcatcgaaag ctctttcatc agctctcgaa acatgtgaac atccgtgaat
      301 ttggaacgga gaaacgtcag tttgtgcttc accagcgggt gttcaaccac cgtcaccttg
      361 ctcatacttc ctcctcgcct agaatcggaa tatagcgttc gagatggcgg ccagaccgtg
      421 tcgagagttc acgtgcaaga ctcgatcttc gccacccgtg cctgatgtcg accgccagca
      481 aacgtgccgc gcaaaaacgt gtcgagaatg tctttcgcca gatcttcgcc aatgatgcgc
      541 gccccgagca ccagaacatt cgcatcgttg tgttcccggg tcaaccgggc ctcgagcgag
      601 ttgtggcaca aggccgccct gatgccatga tgtcggttgg ccgccatcga cataccgatt
      661 cccgacccac agatcaggat accaaacgat gggacctgcg ccagaacgcc tttacagacc
      721 gggtgggcaa aatccgcgta gtcgcaggag gtttccgcat gcgtacccag atcgctgact
      781 tcgtgccctg cctgctgcag ataggccatc aacttgttct tgagaccgaa accggcatga
      841 tctgaaccaa tgaagatttt cacgccttcc cccaggaaaa gtttcctcat gatacacacg
      901 ctctcaggca gaattcaagc ccatttcgac gagaaaatcc gcagcacttt ccgcgttggt
      961 tctgcacgcg atcaccgcac ggttacaggg gatgagcatc tgagccatgc cgccgttatt
     1021 gtcgtggtaa gaatgatgac aaagcaggta tactggcatc gaggcgcgtt ttcaccgcat
     1081 cataccttga cagggatcga acacgtcgtt ggctcaagtg aatcgagtgc tgtggcacgg
     1141 agattgtctg cagaccgccg cgctgatgga gcgtggttcc gtcgatcttg tctatttcga
     1201 ccctccgttc atgaccggga aggaacgcca cggcacacgc caggagttcg ttttcgacga
     1261 ccaatggcag ggagacctcg ccagttttct tccgtggctg cagaaaagaa tcgccgccgt
     1321 cattccgctc gtttcccgcc acgggagtct gatcgtgcat ctcgaccatc gaacgattca
     1381 ctatgtcaaa gtctggctcg atcagcatct tggtgtgaag actttcgtca atgagataat
     1441 ctggcactac accggtggcg ggcgctcccg atctcgtttt tcg
//
LOCUS       DAORHD010001367         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118232_length_1481_cov_3.949509, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001367 DAORHD010000000
VERSION     DAORHD010001367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118232_length_1481_cov_3.949509"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..784
                     /locus_tag="PKO06_21720"
     CDS             14..784
                     /locus_tag="PKO06_21720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV72342.1"
                     /translation="MERREQDERHCLAAALDSAQWADEIVVVDCASTDGTPDVVAAYT
                     KARLFHRPNNPNLNVNKSFGFEQLTTDWVFYLDPDERIPAELAREIRAVIADTPHNAF
                     RLPRRNIFFGRFLAHGGQYPDTQLRLFRRGKARFANKHVHESLDVDGTTGRLSEPFDH
                     LPYPDLESYLRKMIFYTGFQANFWAESGMKPGMINTLRYFCLRPAGRFFRRYLLKRGF
                     QDGWQGFVAAVGDAFQTIISYARFLERQAGTPPANPAA"
     gene            795..>1481
                     /locus_tag="PKO06_21725"
     CDS             795..>1481
                     /locus_tag="PKO06_21725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV72343.1"
                     /translation="MRIIHILNSPNWSGASHYCVTLCDRLGRKGHDVLLLTEPGKPLE
                     RAKKLGIPFDDTIRLNHRNPGLYLHGIKRMEYLFKRFKPDIITSHINEGAWMAGMVAR
                     ESARQAAVVRTRTDIDPPKSHFINRFVHHRWTDHLVVSSQLHKKLCCQLLDFPTDGID
                     VVYGAVDTDTFTPRPALDPIFRREIGATPDTVVIGMVARLDPVKGHEYALQALQMLRA
                     NPIPHLLVVLG"
BASE COUNT          292 a          513 c          406 g          270 t
ORIGIN      
        1 aagttagcag tatgtggaaa ggagagagca agatgagcgg cattgtctcg ccgcggcgct
       61 tgattcagcg cagtgggccg atgagatcgt cgtagtggac tgtgcatcga ccgacggtac
      121 acccgacgtg gtggcggcat acaccaaggc acggcttttt caccggccca acaaccccaa
      181 tctcaacgtc aacaaatcgt tcgggttcga acagctgacc accgactggg tcttctatct
      241 cgaccccgac gaacggattc cggcagaact ggcccgcgag attcgcgcgg tcatcgccga
      301 cacgccccac aacgcctttc gcctgccccg ccggaatatc ttcttcggcc ggtttctggc
      361 gcacggcggc cagtaccccg atacccagct gcgcctcttc agacgcggta aagcgcggtt
      421 cgccaacaaa cacgtgcacg agagcctcga cgttgacggc accaccggtc ggctcagcga
      481 gccgttcgat cacctgccct accccgacct cgaatcgtat ctgcgcaaga tgatcttcta
      541 taccggtttt caagccaact tctgggccga gtcaggaatg aaacccggga tgatcaacac
      601 gctgcgctac ttctgcctgc gcccggcggg ccgtttcttt cgccgctatt tgctcaagcg
      661 cggctttcag gacggctggc agggcttcgt ggctgccgtc ggtgacgcct ttcagacgat
      721 catcagctac gcccggttcc tcgaacggca ggccggaaca ccgccggcca accccgccgc
      781 ctgagcccga ccacatgcgc atcatccata ttctcaattc cccgaactgg agcggcgctt
      841 cgcactactg cgtcaccctc tgtgaccgcc tggggcgcaa gggtcacgac gtactgctgc
      901 tcaccgagcc gggcaagccg ctcgaacgcg cgaagaagct cggcattccg ttcgacgaca
      961 ccattcggct gaaccatcgc aaccccggcc tctacctgca cggcatcaag cgtatggaat
     1021 acctgttcaa gcgcttcaaa cccgacatca tcaccagcca catcaacgaa ggagcctgga
     1081 tggcgggcat ggtcgcccgg gaatcggcac ggcaggcggc tgtagtacgc acccgcaccg
     1141 acatcgaccc gccgaagtct catttcatca accggttcgt gcaccaccgc tggaccgacc
     1201 acctggtggt gtcgtcacag ctgcacaaga agctgtgctg ccagcttctc gacttcccca
     1261 ccgacggcat cgatgtagtc tatggcgcgg tcgataccga cacgttcact ccccgtcccg
     1321 cgctcgaccc cattttcagg cgcgagatcg gcgcgacacc cgacacggtg gtgatcggca
     1381 tggtcgctcg actcgacccg gtcaagggcc atgaatatgc gctgcaggcg ctgcagatgc
     1441 tgcgcgccaa ccccataccg catctgctgg tcgtgctcgg c
//
LOCUS       DAORHD010001368         1480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118346_length_1480_cov_4.324912, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001368 DAORHD010000000
VERSION     DAORHD010001368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118346_length_1480_cov_4.324912"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="PKO06_21730"
     CDS             <1..987
                     /locus_tag="PKO06_21730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72344.1"
                     /translation="AHMQRGLILVLILTWLVPTSMCCAQVQIGYGTEAGQVAFINQVS
                     HPGWEEPLPLGPLAFRATAADLWVADSCGGRVYQFGVDGKLLKELRIATSPDQSLWED
                     IALHRSADGKVSGFSLFNGSTQEIWNCDLNGKVLRKIGGRGEAKGKFLQGTLLEVGPN
                     GTLFVADKARRVISVLAADGTCKREVEWQWSGFFIDAKESLYRLHWNDQEKKTHLLVQ
                     DGEGKQTLDLPLDLPADHFNPELWMVTSQGNLFVTFIDSQGFDGRLKTAEINRQGKVV
                     VSGELTPPLVMNRLLCSSTVPGKYWLANADFNKAPEGQFKVDLVSLPGSEAR"
     gene            complement(1059..>1480)
                     /locus_tag="PKO06_21735"
     CDS             complement(1059..>1480)
                     /locus_tag="PKO06_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72345.1"
                     /translation="LSIWGGEIANVGANIMSLPPMVDAPGDGDADTSETAIITGTSGD
                     FVLPPPPPPPPFDPGMKVIDGATEAKPPVSITPDSGDTEGFSLPAPPPLPGMEPASGK
                     APGKKSKVDPAPAAATPASSGEFMLPPPPSLPGFEKK"
BASE COUNT          295 a          393 c          475 g          317 t
ORIGIN      
        1 gctcatatgc aacgaggatt gattctggtt ctgattctga cgtggttggt accgacctcg
       61 atgtgctgcg cgcaggtgca gatcggatac ggcacggaag ccgggcaggt ggcctttatc
      121 aatcaggtaa gccaccccgg ctgggaagaa ccccttcctc tcggaccgtt ggcttttcgc
      181 gcgacagccg cggatctctg ggttgctgac agttgtggcg gccgggtcta ccagtttggc
      241 gtagacggca agctgctgaa ggaactgcgc atcgccacct ctcccgatca atctctctgg
      301 gaagacatcg ctctgcatcg ctccgccgac ggcaaggtca gcggtttttc tctcttcaac
      361 ggttcgacgc aggagatctg gaattgtgat ctgaacggga aagtgttgcg aaagatcggt
      421 ggacgcggcg aagcgaaagg aaagtttctg caggggacgc ttctcgaggt ggggccgaac
      481 ggaacgctgt tcgtcgccga caaagcccgt cgcgtgatct cggtgctcgc tgccgacggg
      541 acctgtaagc gggaagtcga gtggcagtgg tctgggttct tcatcgacgc gaaagaaagt
      601 ttgtatcgct tgcactggaa tgatcaggaa aagaagactc atctgctggt acaagacggc
      661 gagggcaagc agacgctcga tttgcctctc gatctcccgg cggatcattt caacccggaa
      721 ctctggatgg tcacctcaca gggaaatctg ttcgtgactt tcattgattc ccagggattc
      781 gatggtcgtc tcaagacggc ggaaatcaac cgtcagggga aagttgtggt ttcaggagaa
      841 ctgacgccgc ctctggtcat gaatcgcctg ctttgctcat cgacggtacc cggaaaatac
      901 tggctcgcga acgctgactt caacaaagcc ccggaagggc agttcaaggt cgatctggta
      961 tctctgccgg ggagtgaagc caggtaaaat ctgattgaac aggaaaaccg cccgactcga
     1021 aagtcgggcg gtttttcatc tctggttgtg gaaaggcgtt acttcttctc gaacccgggg
     1081 agcgaaggag gagggggcag catgaattcg ccgctcgatg ccggggtcgc tgcagcaggt
     1141 gctggatcga ctttggattt cttccctgga gctttcccag aagcgggttc catgccgggt
     1201 aacggtggtg gggccgggag agaaaacccc tccgtgtccc ccgagtcggg ggtgatcgat
     1261 accggcggct tcgcctcggt tgccccgtcg atgaccttca tccccgggtc gaacggggga
     1321 ggcggaggcg gtggtggcag aacgaagtcg ccgctggttc cggtgatgat ggcggtttcc
     1381 gaggtgtcgg catccccatc ccccggcgcg tcgaccatcg gcggcagact catgatgttg
     1441 gcaccaacgt tggcgatttc gccgccccag atcgataacg
//
LOCUS       DAORHD010001369         1479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118478_length_1479_cov_4.061798, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001369 DAORHD010000000
VERSION     DAORHD010001369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118478_length_1479_cov_4.061798"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..614
                     /locus_tag="PKO06_21740"
     CDS             201..614
                     /locus_tag="PKO06_21740"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNV72346.1"
                     /translation="MKHWIMLFTPETYELVKKHSTIGVRSNVWKSFSENMKVGDRFIG
                     YVSRQMIFDATGTITGDATFEETMLFHDQKWFPCRRRVKFEKTGLNKPSSDLFHGVAP
                     FNEVNTGPGNYLMCKGGFVEVSKKDFDWLCGKLGL"
     gene            640..>1479
                     /locus_tag="PKO06_21745"
     CDS             640..>1479
                     /locus_tag="PKO06_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV72347.1"
                     /translation="MVKLEEIKNSAFLNGIEPGQAVRVVTTEPVGDNALTVYYKTVDG
                     QTRDRMLFRSDEAGLSLAQAGRPWAFDAPGADFKLVTEAWRINTAYLFDPMMAVHTSN
                     VEPLPHQITAVYESLLPRQPLRYVLADDPGAGKTIMAGLYIRELILRADARRVLIVAP
                     GGLVEQWQDEMEEKFGLSFSLFTREMQDQSRSGNPFDDHDLLIARLDQLSRSEEIQEK
                     LRHTQYDAIIVDEAHKLSATWFGSKVNETKRFKLGRLLGSITRHFLLMTATPHNGKEE
                     DFQL"
BASE COUNT          347 a          429 c          400 g          303 t
ORIGIN      
        1 cctgcggtcc cggtgtgcca ccagaaaatc gaggcatacg agacaaccac cgaaattcta
       61 ccaaagcctg cgaaagaatc aacggaaatc gagccgaggt ggtcccggaa tccgttgatt
      121 accccggtat cacgtgataa cctgatgctc acatttccgg agaggcaaca gagcatcgaa
      181 aactctttga ggagattcac gtgaagcact ggatcatgtt gttcacaccc gaaacgtatg
      241 aactggtcaa gaaacattcg accatcggcg tgcgttccaa cgtctggaag agcttttccg
      301 agaatatgaa ggtcggtgat cggtttatcg gctacgtctc gcgccagatg atcttcgacg
      361 ccaccggcac gattaccggt gatgcgacgt tcgaagaaac catgcttttc catgatcaga
      421 agtggtttcc gtgtcgtcgt cgagtgaagt tcgaaaagac cgggctgaac aagccgtcaa
      481 gtgacctgtt tcacggcgtt gccccgttca acgaggtcaa taccggtcca ggcaactacc
      541 tgatgtgcaa gggcgggttt gtcgaggtgt cgaagaaaga cttcgactgg ctgtgcggca
      601 aactgggtct ctgacctcat ttccggctat gcagacgcca tggtaaaact cgaagagatc
      661 aagaacagcg cttttctgaa cggcattgag ccaggtcagg cagttcgcgt cgtcaccacc
      721 gaaccggttg gcgacaacgc cctcacggtc tactacaaga ccgtcgacgg gcaaacccgt
      781 gatcgaatgc tgttccgctc tgacgaagcc ggtctcagcc tggcgcaagc cgggcggccc
      841 tgggccttcg atgcccccgg agctgatttc aaactcgtca ccgaagcgtg gcgtatcaat
      901 acggcgtatc tttttgaccc gatgatggcg gtacacacct cgaacgtcga gcctctgccg
      961 catcagatca ctgcagtcta cgagtccctt ctcccccgcc agccattgcg atacgttctc
     1021 gccgacgacc ccggtgcagg caagaccatc atggcaggtc tctacatccg cgagctgatt
     1081 ctgcgtgccg atgccaggcg cgtgctgatc gttgcccccg gtggtctggt cgagcagtgg
     1141 caagacgaaa tggaagagaa gttcggcctc tcattcagtc tgttcacgcg agagatgcag
     1201 gaccagagtc gttcagggaa cccttttgac gaccatgacc tgctcattgc acggctggat
     1261 cagctctccc gtagcgaaga aatccaggag aaactgcgcc acacccagta tgatgccatt
     1321 atcgtcgacg aagcccacaa gctctcggca acctggtttg ggagcaaggt caacgagacc
     1381 aagcgcttca agctgggccg tctgctcggc tcgatcaccc gccattttct gctgatgacc
     1441 gcaaccccgc acaacggcaa ggaagaagac tttcagctc
//
LOCUS       DAORHD010001370         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118622_length_1478_cov_3.903725, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001370 DAORHD010000000
VERSION     DAORHD010001370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118622_length_1478_cov_3.903725"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="PKO06_21750"
     CDS             complement(<1..931)
                     /locus_tag="PKO06_21750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNV72348.1"
                     /translation="MDEQLAEILALCKKLDASDVHLRVGFPPVYRVAGQIQITSGLPL
                     SVETLRKFFESTAPAVTQKMLEVRKDADYSVSLPGVSRFRVNCFQQRGTLAFVFRQIP
                     FTLPTIDSMKIPRIMVDAVQRNSGLFLLCGPTGSGKSTTIAALLQFINTSYNVRIVTL
                     EDPIEFLFRDEKAEFLQRELRRDFTSFPEALRGTLRQDPDVIVVGEMRETETIELALT
                     AAETGHMVISTLHASSSASAVARILGVFPTDAREFIRDQLAAVLVGIVAQALIPSKKD
                     PNRRVAAREILLNNPAIANLIREAAKGPSPFSEC"
     gene            complement(1236..>1478)
                     /locus_tag="PKO06_21755"
     CDS             complement(1236..>1478)
                     /locus_tag="PKO06_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72349.1"
                     /translation="NGYRLLDGELRARVNGREETLMRRPDRLVYKTDDVTAELDIYSL
                     AVREARFKHGDVAAVSFEHAVRMAVLLKSLQNASFR"
BASE COUNT          318 a          399 c          445 g          316 t
ORIGIN      
        1 ggcattccga gaagggactt gggccttttg cggcttcgcg gatcaggttc gcgatggccg
       61 ggttgttcaa gagaatttcg cgcgcggcga cccggcggtt cgggtctttc ttcgagggaa
      121 tgagcgcctg ggcaacaata ccgaccagga cggcggccag ctgatcgcga atgaactcgc
      181 gggcgtcagt cggaaacacc cccagaatgc gggcgacggc cgaagccgaa ctgctggcat
      241 gcagggtcga gatgaccata tgtcccgttt cagcggcggt gagcgctagt tcaatggttt
      301 ctgtctcacg catctcgccg acgacgatga cgtcagggtc ctggcgcagg gtgccgcgca
      361 gcgcctcggg aaagctggtg aagtcgcgac gcagttcgcg ctgcagaaac tcggcttttt
      421 cgtcacggaa gagaaactcg atcggatctt cgagcgtgac gatgcgtacg ttgtagctgg
      481 tattgataaa ctgcaacaga gcggcaatcg tcgtgctttt ccctgaaccg gtgggaccgc
      541 agagcagaaa cagaccggaa ttgcgttgca cggcatcgac catgatgcgc ggaatcttca
      601 tggagtcgat cgtgggcaga gtgaagggga tctgacgaaa aacgaaggcc agggttccgc
      661 gttgttgaaa acaattgacg cgaaatcgac tgacgccggg cagagacacg gagtagtccg
      721 cgtcttttct gacttccagc attttttgcg tgacggccgg cgcggtgctc tcaaagaatt
      781 ttctgagcgt ttccacggag aggggaagcc cggacgtaat ctgaatctga ccggcgacgc
      841 ggtacaccgg aggaaacccg acccgaaggt ggacatcgga agcgtcgagc ttcttacaga
      901 gagcgaggat ctccgccagt tgttcatcca tgcaggaatc ccttcatgtc gagcagtgcg
      961 tacctaaacc gtcaaaccgt catacgtaat gatacgaggt gaacgttcct ttttcatagt
     1021 atacctcacg gcatttcctc caacaacttg tatgtgcccc agagttatca gattcgccaa
     1081 aggttatgtt ttccctctcg cactgctcaa cgcaaaatct ttcaagggag aagcgacccg
     1141 ttctgcctca aggaaaaggt gtttcgcttg tctcagagcc ttcgaccggt tgctgagacg
     1201 cttccagtcg aaggcatgac tcttttcttt ctttatcagc ggaagctggc attctgcaga
     1261 gacttgagca acacggccat gcgcaccgca tgctcgaacg agacggcggc gacatcgcca
     1321 tgcttgaatc gggcttcacg cacggcaagg ctgtagatat cgagctcggc cgtcacgtcg
     1381 tcggttttgt acaccagacg gtcggggcga cgcatgagcg tctcttcgcg gccgtttacc
     1441 cgggcgcgca gttcgccgtc gagcaggcgg tacccgtt
//
LOCUS       DAORHD010001371         1478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_118676_length_1478_cov_2.708363, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001371 DAORHD010000000
VERSION     DAORHD010001371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118676_length_1478_cov_2.708363"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKO06_21760"
     CDS             complement(<1..259)
                     /locus_tag="PKO06_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72350.1"
                     /translation="MNGNLGRTLALVLFFSLAALGCLQAQDSATPGKSRVEVVFCLDT
                     TGSMGGLIAGAQQKIWSLVNGIATGKPVPEGSALPPNPLNQY"
     gene            complement(355..933)
                     /locus_tag="PKO06_21765"
     CDS             complement(355..933)
                     /locus_tag="PKO06_21765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72351.1"
                     /translation="MCAQSLGTDTKASTIVVREYAQGTLKHPARIIEARYKSSHQKPP
                     SVTFNQGTIGPIQYASPEEVVGLEILPHTPYAFSTQKGVTLSFVYEPIPDAAETSTIP
                     PEQNALEIEADNSDQTFEQKFKLDSFVQAGGFNTDAPSLVITVDGNILPENMFQPASA
                     ALGAFIVEPGHTYRFKAPVGSRLILRFMPVSP"
     gene            1188..>1478
                     /locus_tag="PKO06_21770"
     CDS             1188..>1478
                     /locus_tag="PKO06_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72352.1"
                     /translation="MSLSERIGDIGRDQSDRGTISITPEFRVPTLLGTTLRIVLAVAF
                     IALYFYGLHEYYGVLNRKRLLYACWGLFVYAFFKFSWSIRQWVKQAERDAGGG"
BASE COUNT          314 a          403 c          410 g          351 t
ORIGIN      
        1 tgtactggtt cagcgggttg gggggcagcg cgctgccctc gggcaccggc ttcccggtgg
       61 cgatcccgtt gacgaggctc cagatcttct gttgcgcgcc ggcaatcagc ccgcccatgc
      121 ttcccgtcgt atcgagacaa aagacgactt cgacccgcga ctttccgggt gttgcgctgt
      181 cctgcgcctg cagacaacct agggcggcaa gactgaaaaa gagcacgaga gccagcgttc
      241 gaccaagatt tccgttcatg cgatttcctc cccgaagttt gtgtggctaa cgtttcacca
      301 aacgccctcg cgagcattcg ggtttagagc aaggtttctt gcgagagatg ctgatcatgg
      361 cgaaaccggc atgaaccgaa ggatgagcct cgaaccaacc ggtgctttga aacggtaggt
      421 atgacctggt tcgacaataa atgcgccaag agccgctgac gctggctgaa acatattctc
      481 cggcaatatg tttccatcga cggtaatgac gagacttgga gcatcggtgt taaaccctcc
      541 tgcctgcacg aacgaatcaa gcttgaactt ctgctcgaaa gtctggtctg aattgtcggc
      601 ctcgatctcg agagcgttct gttctggcgg gattgtcgaa gtttcggcag catcagggat
      661 cggctcatag acgaaactga gcgttacgcc cttctgagtg gaaaaagcat acggagtatg
      721 gggcagaatc tcgagcccga caacctcttc cggtgaagca tactgaatcg ggccgatggt
      781 tccctggttg aaggtcacgg atggcggttt ttgatggctg gacttgtagc gcgcctcaat
      841 gattctcgct gggtgcttca gagtgccctg ggcatactcg cgaaccacga tcgtactcgc
      901 tttggtgtcg gtaccgagag attgtgcgca caccaaaggt acaaccccgc atacggtcat
      961 agcaattatg agtagcactc tcagctcaaa atgaacggca tttctcatga acctcttatc
     1021 tcccctgtaa agatcgctgt cgccaacgga ctatatcaca aatccggcgt tcattgcgcc
     1081 ctgcgtttca ctcattgccg gttgtggttt tgaatagata ctatgtatca ttctgtcgcc
     1141 ggcttcgcga gagacagcac gggaaatcat ggctcgaggt gcttgtcatg agcttgtctg
     1201 aacgtatcgg agacatcggg agagatcaga gcgaccgcgg aactatctcg attaccccgg
     1261 aattccgtgt ccccaccctg ctgggaacaa ctcttcgcat cgttctggcc gtcgccttca
     1321 tcgccctcta tttctatggc ttgcacgaat actacggcgt tctgaaccgc aagagactgt
     1381 tgtatgcctg ctgggggctg ttcgtctacg ccttcttcaa gttctcctgg tcgatccggc
     1441 agtgggtcaa acaagccgag cgcgatgcgg gcggcggg
//
LOCUS       DAORHD010001372         1475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119074_length_1475_cov_3.262676, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001372 DAORHD010000000
VERSION     DAORHD010001372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119074_length_1475_cov_3.262676"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKO06_21775"
     CDS             <1..611
                     /locus_tag="PKO06_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009108722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72353.1"
                     /translation="SIGLVQWKSGKRSSWRSASARMLDRRLVKPEHPCLSDIGRLDHC
                     ATRVRAQERDAAILEFLNLTNYHFDFAVYVDSLNSITNVSRLGVNDFALVFTSGISPF
                     VDEATSGPTEKFAHTHGPRVHHLAFDCRNIENVVSELEKHGQRYLEKLVGSREEGLKQ
                     IFTVASKHTLLVNEYIQRYDGFDGFFTKSNVTKLTRATLQQD"
     gene            611..>1475
                     /locus_tag="PKO06_21780"
     CDS             611..>1475
                     /locus_tag="PKO06_21780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HNV72354.1"
                     /translation="MKSLKELFRIGNGPSSSHTMGPKKAAEIFLSRHAEAASFTVTLY
                     GSLAATGKGHFTDTAILSVLEPHAKTEIVWQPKVVLPRHPNGMNFKAFDSAANELDSW
                     TVFSVGGGALADEDGKPVGMEEGQGVEIYEMNTMTELLSWCEKGGRSYWEYVRESEGS
                     DIWDYLEEVWLTMKSAVQKGLQEEGSLPGPLNLPRKAVSYFVRASGYTQTLKSRGLVF
                     SYALAVSEENASGGKIVTAPTCGSSGVVPAVLYHLQTTRDFSTARILRALATAGLIGN
                     IVKHNASISGAE"
BASE COUNT          323 a          422 c          437 g          293 t
ORIGIN      
        1 actcgatcgg actggtacaa tggaaatcag gaaaacgatc gagctggcgt tccgcgtcgg
       61 cgcggatgtt ggaccggcgt ctggtcaaac ccgagcatcc ctgtctgagc gatatcgggc
      121 gtcttgacca ttgcgcgacc cgcgtgcgcg cacaggagcg tgatgcggcg attctcgagt
      181 ttctcaacct gaccaactat cactttgact ttgccgtcta cgtcgattcg ttgaactcga
      241 tcacgaacgt ctcgcgactg ggcgtgaacg acttcgcgct cgtgtttacc tcgggcatct
      301 caccgttcgt tgatgaggct acgtcagggc cgacggagaa gttcgcgcat acccacggtc
      361 cacgcgtgca tcatctggcg ttcgactgcc gcaacatcga gaacgtggtt tccgagctcg
      421 aaaagcatgg gcaacgctat ctcgaaaaac tcgtcggcag ccgcgaggag ggcctcaagc
      481 agatcttcac cgttgcctcg aaacacacgc tgctggtgaa cgagtacatt caacgctacg
      541 acggcttcga tggtttcttc accaagagca acgtgaccaa gctcacccgc gccaccctgc
      601 aacaagactg atgaagtcac tgaaagagct ttttcgcatc gggaacgggc cttcgagcag
      661 ccacaccatg gggccgaaaa aggccgccga gatttttctc agtcggcatg cagaggcggc
      721 ttccttcacg gtgaccttgt acggaagtct cgcggctacg ggcaagggtc acttcacgga
      781 taccgccatt ctctcggttc tcgagccgca cgcgaagacg gagatcgtct ggcagccgaa
      841 ggtggttctg ccccggcatc cgaatggcat gaatttcaag gccttcgatt ccgccgcgaa
      901 cgaactcgac agttggacgg tgttcagcgt cggtggcggc gcactggccg acgaggacgg
      961 aaaaccggtc ggcatggagg aaggacaggg cgtcgagatc tacgaaatga acacgatgac
     1021 cgaacttctt tcctggtgtg aaaaaggggg aagaagctac tgggagtatg tgcgagagtc
     1081 cgaagggtcg gacatctggg actacctcga ggaagtctgg ctgaccatga aatcggccgt
     1141 gcaaaaaggt ttgcaggaag aggggtctct ccccggccca ctgaacctgc ccaggaaggc
     1201 ggtgtcttac tttgtccgag ccagcggcta tacgcagacg ctcaagtcgc gagggttggt
     1261 cttttcgtat gcgctcgccg tcagcgagga aaacgcctcc ggtggaaaaa tcgtcacagc
     1321 gcccacctgc ggctccagcg gggtagttcc ggctgtcttg tatcatctgc agaccacgag
     1381 ggacttcagc actgcccgga ttctgcgtgc cctggcaact gccggtctca tcggcaacat
     1441 cgtcaagcat aatgcctcca tatcaggagc cgaag
//
LOCUS       DAORHD010001373         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119193_length_1474_cov_3.818182, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001373 DAORHD010000000
VERSION     DAORHD010001373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119193_length_1474_cov_3.818182"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKO06_21785"
     CDS             <1..255
                     /locus_tag="PKO06_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72355.1"
                     /translation="KPIIAAVPPGENRDFLAGWPGVWIVRPDSLAELADAVEEVWNLR
                     RKDSALRFDRSARTREFEYHVLGKKLRTLIDNVCANGRTR"
     gene            252..1085
                     /locus_tag="PKO06_21790"
     CDS             252..1085
                     /locus_tag="PKO06_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV72356.1"
                     /translation="MTDEKTSDCIEIRAGWAERDYVSDLWRFRELFFFLAWRDIQARY
                     KQTAIGVAWSLIKPIVTMIVFSVIFGRLARLPAGELPYPVLVFAALLPWQFFANALAA
                     TSESLLGNAQLITKVYFPRLIIPVSSVIVGLVDFLLSFLVFLCLLAWYAYLPPLRVCF
                     MPLFLLLGFVNALGFGVWFAALNVRYRDFRHLIPFVIQIGLYISPVGFSSAVIPEEWR
                     LLYCLNPMTGVIDGFRWCLQPQATLYWPGLCLATVAGLLVLVSGMTNFRRLERSFADE
                     I"
     gene            1089..>1474
                     /locus_tag="PKO06_21795"
     CDS             1089..>1474
                     /locus_tag="PKO06_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV72357.1"
                     /translation="MTASRNLPVIEVQGLGKRFYLEQWKKNPDSRSLQFQFGQWLSSM
                     FSPSADEQGWGATEKEEFWALREIEFSVQAGERVAIIGRNGAGKSTLLKILSRITEPT
                     EGLARLRGRVASLLEIGTGFHFELSGR"
BASE COUNT          267 a          373 c          433 g          401 t
ORIGIN      
        1 aagcctatca ttgctgcggt cccgcccggt gaaaatcgtg attttctggc cggctggccg
       61 ggagtctgga tcgttcggcc cgattcattg gctgagttgg ctgacgcggt ggaagaggta
      121 tggaatctgc gacggaaaga ttcagcactg cgttttgatc gttctgcgcg cacgagggaa
      181 ttcgagtatc atgttctcgg caagaaactg cgcacgttga ttgacaacgt gtgcgcgaat
      241 ggacgaacca ggtgactgac gagaaaacca gcgattgtat cgaaattcgt gcgggctggg
      301 ctgagcgtga ctacgtctcg gatctctggc gttttcgtga actgttcttc tttctcgcct
      361 ggcgtgacat tcaggcacgt tacaagcaga cggcgatcgg tgtggcctgg agcttgatca
      421 agccgattgt caccatgatc gtgttttccg tgatctttgg tcgcctggcg cgactgcccg
      481 cgggtgagct tccatacccg gttctggtgt ttgcagccct gttgccatgg cagttcttcg
      541 ccaatgcgct tgccgccacc agcgaatcgc tgctgggaaa cgcccaattg atcacaaagg
      601 tgtattttcc tcgcttgatt attccggtca gttcggtgat cgttggtctg gttgattttc
      661 tgctttcatt tttggtcttt ctctgcctgc tggcatggta tgcctatctg cccccactcc
      721 gcgtctgttt catgccgctg tttctgctac ttgggttcgt gaacgcgctg ggcttcggtg
      781 tctggtttgc cgctctcaat gttcgatatc gcgattttcg gcatttgatc ccttttgtaa
      841 ttcaaattgg cttgtacatt tctccggtcg gattctcgag cgcagtcatt ccggaggaat
      901 ggagactgtt gtactgtctg aacccaatga cgggagtcat tgacgggttc cgttggtgtc
      961 ttcaaccgca ggcaaccctc tactggccgg ggctttgcct cgccacggtc gctggtctgt
     1021 tggtgctggt ctccggaatg accaacttcc gtcgacttga acgatccttt gcagacgaga
     1081 tctgaccgat gacagcgagc agaaatctac cggtcatcga ggttcagggg ttagggaaac
     1141 gtttctatct cgaacaatgg aaaaagaatc ccgacagtcg cagtctccag ttccagttcg
     1201 gacagtggct ttccagcatg ttttctccct cggctgatga gcaggggtgg ggagctacgg
     1261 aaaaggaaga gttctgggct ctgcgcgaga tcgagttctc ggttcaggcc ggcgagcgag
     1321 ttgcgattat tggccgcaat ggtgccggca agtcgacgtt gctgaagatt ctgagtcgta
     1381 tcacagaacc gaccgaaggg ctcgcgcgat tacgcggcag agtggcgagt cttctcgaaa
     1441 tcggcaccgg gttccatttc gaactttcgg gccg
//
LOCUS       DAORHD010001374         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119210_length_1474_cov_3.098661, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001374 DAORHD010000000
VERSION     DAORHD010001374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119210_length_1474_cov_3.098661"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKO06_21800"
     CDS             complement(<1..684)
                     /locus_tag="PKO06_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNV72358.1"
                     /translation="MGEPLDKYHPPYQITPKIVHLIAEISEMIGGFAALAEPNLTPRL
                     RRDSRIRTIQASLAIENNTLSLEQVTAVIEGKRVLGHPREIQEVRNAFAAYEAMDTWN
                     VHSERDLRAAHAVLMRGLVDDAGRYRTSGVGIYRGKQLVHMAPPANRVSRLMVNLFDW
                     LSKTDEHPLVASCAFHYEFEFIHPFADGNGRMGRLWQTLMLRSWKPLLSYLPIETVIR
                     DRQKEYYRLL"
     gene            complement(1013..>1474)
                     /locus_tag="PKO06_21805"
     CDS             complement(1013..>1474)
                     /locus_tag="PKO06_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72359.1"
                     /translation="ILVTVVTRNRVKQLDKALTGRIIAFFDWRFENAEPLELQEFTFW
                     LEAECLESEWRLKSYSKILDVGRGEDVSLSLQVRALNKLLPNHLALVVECFAKITDAM
                     DQSTQLYIAADESKPILKAGLVAEDSKVRENAERARENLLRRGCFDYLDVV"
BASE COUNT          371 a          415 c          336 g          352 t
ORIGIN      
        1 caggagacgg tagtattctt tctgccgatc tcgaatcact gtctctatcg gcagataaga
       61 taacaacggc ttccagctcc gcaacatcag agtttgccat aatcgcccca ttcgaccgtt
      121 gccgtctgca aaagggtgaa tgaattcaaa ttcgtaatga aaagcgcaac tcgccaccaa
      181 cggatgttca tccgtcttac tcagccagtc aaacaggttg accatcaaac gcgaaacccg
      241 gttcgccggc ggagccatat gtaccagttg cttccctcga tagatgccga ctccactggt
      301 tcgataccgc ccggcatcgt cgaccaaccc acgcatgaga acggcatgag ccgcccgcaa
      361 atcacgttca gaatgaacat tccatgtgtc catcgcttca taggcggcaa acgcgttccg
      421 gacttcctgt atttcgcggg ggtgccccaa tacccgcttg ccctcaatga ccgcggtcac
      481 ctgttcaagc gagagcgtgt tgttctctat cgccaatgac gcctgaatcg tgcgaatacg
      541 actgtcacgg cgtagacgtg gcgtcaggtt cggttcagcg agtgcagcaa agccgccaat
      601 catctcactg atttcggcaa tcagatggac gatcttcgga gtgatctgat agggcggatg
      661 atatttgtcc aaaggctctc ccatgaaaat gcccttcgcc aaaggcgaag ggcattcatc
      721 gaatggaggc ggcgtctaca gaactaccac cagcaatact tcttccaaca agacttttgc
      781 gctttcacaa agtctccgtt accccacatt gttaccccaa attaacgcaa gcgcattagt
      841 tttcgcccga gcactcggtt catttactat tataccatcg aaacaacgaa aatcttgtct
      901 tgattgtggt aagaactttt tatctcggaa atagaagatc tattagccgg aaatcgagat
      961 ccacttgttg tctgctcgat actctgctgc aattgtcata aggccggatc cctcagacaa
     1021 cgtccagata atcaaagcaa ccgcgccgca acagattttc ccgtgcacgt tcagcatttt
     1081 cacgcacctt agagtcttca gcaaccaggc cagccttcaa gatcggcttg gactcgtcgg
     1141 ctgctatgta cagctgtgtg ctttggtcca tggcatccgt gattttggca aagcattcta
     1201 caaccaatgc caggtgattc gggagaagtt tgttcagggc tctcacctga agcgagaggc
     1261 tgacatcttc tccacggccc acatcgagaa tcttcgagta agatttcaaa cgccattcag
     1321 actcgaggca ctcagcttcc agccaaaagg tgaactcctg aagctccagc ggttcagcgt
     1381 tttcaaaccg ccaatcgaaa aaagcgatta tgcggccggt cagcgcttta tccagttgct
     1441 tcacgcggtt gcgcgtcacg acggtgacta aaat
//
LOCUS       DAORHD010001375         1474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119243_length_1474_cov_2.622269, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001375 DAORHD010000000
VERSION     DAORHD010001375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119243_length_1474_cov_2.622269"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKO06_21810"
     CDS             <1..463
                     /locus_tag="PKO06_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72360.1"
                     /translation="LISISVILCLAITACLHYDLFSRRTVYPADPTLSFEEFASIHWF
                     GRKRPVLLFLLAFAKDTDLRLKYIGYAGSRLLIFIGIVAVMSWFGLANSSLFERFYSA
                     WFPWFPYGLVVIGAVTCLYLFLRREYSIYRRLRVPSGPDDSVVTSRETPRA"
     gene            538..>1474
                     /locus_tag="PKO06_21815"
     CDS             538..>1474
                     /locus_tag="PKO06_21815"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV72361.1"
                     /translation="MLSALIVLGVTVSSVSASSIDYERLQALCASDSFDVVVVGGDPE
                     GIAAAIGAARAGARTLLVDTRSIPGGLLTRGWLNTIDLNLDRRGRPLNGGLFGELFSR
                     LDDHSFDVATMQELLEKMIENETNLSYYPGALSVLPIVGSDSRRLYQPGQTLCPDDRQ
                     LSPDWFTEVPPQNPPASVPLSIQGVLIGQDRSQTVRVRAHTVIDATQDADLAVAAGAS
                     FIRYGEDVWGSPRNMAATLVFRMSGVSTADWERMCEDLRRKPGAAGLIGGTHRSVWGY
                     GDVVQEYKPSSERIRLRALNMGRQNDGSILVNALLV"
BASE COUNT          260 a          423 c          416 g          375 t
ORIGIN      
        1 tctgatttcg atttcagtga ttctgtgcct ggcaatcact gcctgcctgc attacgatct
       61 cttttcgcgc cgaaccgtct atcccgcaga tcccacgtta tcattcgagg aattcgcctc
      121 tattcactgg tttggccgca agcgaccggt acttctgttt cttttggcat tcgcaaaaga
      181 caccgatctg cgcctgaaat acatcggcta cgccggctct cgtctgctga ttttcatcgg
      241 tattgtcgcg gtcatgtcct ggtttggcct ggccaacagc tcgctctttg aacgctttta
      301 cagcgcctgg tttccctggt ttccctacgg tctggtcgtc attggtgccg tcacctgcct
      361 gtatctcttt ctccggcgcg agtattcgat ctatcgccgt ctcagggttc catccggtcc
      421 tgatgactcg gtggtcacca gcagagagac accacgcgca tgatgtcaat ccattggctg
      481 gaacagtggt ctttcagatt cttcaggtca gcgcctggtt tcttgacagg acgattcgtg
      541 ttgtcagctc tgatcgttct tggcgtcacc gtttcatctg tatcagcgtc atccattgat
      601 tatgagagac ttcaagctct ttgtgcctcg gactcttttg acgtcgtggt tgtcggcggc
      661 gatcccgaag gaattgccgc ggccattggt gcggcgcgag ccggtgctcg taccctgctg
      721 gtcgacaccc gctcgattcc cgggggactt ctgacacgcg ggtggttgaa tacgatcgat
      781 ctcaatctcg atcgccgagg tcgaccgttg aacggtggcc tgttcggcga attgttcagc
      841 cggctggatg atcattcatt cgacgtcgca accatgcagg agttactcga aaagatgatc
      901 gaaaacgaaa ccaatctttc gtattatccc ggagctctgt ccgtgctccc gatcgtcgga
      961 agtgactcca gacgactgta tcagcccgga cagactctgt gtccggacga tcgtcagctg
     1021 tcgccggact ggttcaccga ggtgccaccg cagaacccgc cggcgtcggt cccgttgtcg
     1081 atacaggggg tgttgatcgg tcaggatcga tctcagacgg ttcgggtcag ggcacacacg
     1141 gtcatcgacg ccacgcagga tgccgatctg gccgttgccg ccggagcgtc tttcatccgg
     1201 tacggcgaag acgtctgggg cagcccgcgc aacatggccg ccactctcgt gtttcgcatg
     1261 tctggcgtgt cgaccgctga ctgggaacgg atgtgtgaag atcttcgccg caaacctgga
     1321 gctgccggct tgatcggcgg cacacatcgc agcgtctggg gatacggtga tgtggtgcag
     1381 gagtacaagc catcatcaga gcgaatacgc ttgcgagcct tgaatatggg ccggcagaac
     1441 gacggcagca ttctggtgaa cgccctgctg gtct
//
LOCUS       DAORHD010001376         1471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119604_length_1471_cov_3.318503, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001376 DAORHD010000000
VERSION     DAORHD010001376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119604_length_1471_cov_3.318503"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKO06_21820"
     CDS             <1..583
                     /locus_tag="PKO06_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV72362.1"
                     /translation="GKKPTNIVFMGMGEPFFNREAVFGAIDTLTRPEGLALATRRITV
                     STVGVPAGIRAFSERPGEVNLAVSLHAADDTTRSRLVPLNKKYPLKKLREAIEEYLAA
                     TNRRVTFEVALLQGVNDGLDNVLNLVQFCEGLLCHVNLIAFNPFPGCGYKPAPEDAVR
                     EFKKQLKKAGVPVTLRRSKGADIMAACGQLAGK"
     gene            587..>1471
                     /locus_tag="PKO06_21825"
     CDS             587..>1471
                     /locus_tag="PKO06_21825"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNV72363.1"
                     /translation="MAKQESCLLILVKMILMVTLLLAGLIAGLFFLRDKLNEYFNRGE
                     NIPVPDFRGKHLVEVFKEKPADLIIEKRDEKFDPLVPKDHVIAQYPEPGIRVKPNKKI
                     LLTISMGTKQVNVPDLLEKNQREVTLALINNQLREGNRAFISSSRFPREKVIMQSPLP
                     HSPHQIQGGVDLLISLGGTSARAPLPNFLGKGVVEAKASLQALGLTIGKTITKKDPSR
                     TKDTVIMTRPGPYDPVTENATVDLLVSSGLDAGTATAEDLKRFETVEGPLPSNTPPAP
                     PTIKIAASATSVVKLPPQT"
BASE COUNT          340 a          442 c          395 g          294 t
ORIGIN      
        1 cgggaaaaag ccgacgaaca tcgtcttcat gggcatgggc gagccgttct tcaatcgtga
       61 ggcagttttt ggcgccatcg acaccctgac ccgccccgaa ggtctggctc tggcgacgcg
      121 ccgcatcacc gtctcaaccg tgggagttcc cgctggcatt cgcgccttca gcgagcgtcc
      181 gggtgaggtc aatcttgccg tctctctgca tgcggctgat gacacgactc gttcacggct
      241 ggtgcctctc aacaaaaagt acccgctgaa aaagctgcga gaagcgattg aagagtatct
      301 ggcggcgacg aatcgacggg tcacctttga agtcgctctg cttcagggag tcaacgatgg
      361 gctcgacaat gtcctgaacc tggttcaatt ctgcgaaggg ttgctctgtc atgtcaacct
      421 gatcgctttt aatccgttcc cgggttgcgg ttacaaacca gcaccagaag acgccgtgcg
      481 ggaattcaaa aaacagctga aaaaagccgg ggttccggtg accctgcgac gatcaaaagg
      541 agccgacatc atggctgcct gcgggcagct tgccggaaag taggatatgg ccaaacaaga
      601 atcctgtctg ctgatcctgg tcaagatgat cctgatggtg acgctgctgc ttgccgggct
      661 catcgccggt ctgttcttcc tgcgcgacaa actcaacgag tatttcaatc gcggtgagaa
      721 cattccggtt ccggactttc gggggaagca tctggtcgag gtattcaagg aaaagccagc
      781 ggacctgatc atcgaaaaac gcgatgagaa gttcgatccg cttgtgccga aagaccatgt
      841 catcgcccag tatcccgagc cgggaatccg ggtcaagccg aacaagaaga ttcttttgac
      901 catctccatg ggcaccaagc aggtgaacgt tccagatctg ctcgaaaaga atcagcgtga
      961 agtcacgctc gccctgatca acaaccagct tcgcgaagga aaccgcgcat tcatttcgtc
     1021 gtcacggttt ccccgtgaga aagtcattat gcagtctcca ctcccgcact cgccacatca
     1081 gatccaggga ggcgtcgatc tgctcatcag tctcggtggc acctcggccc gggctcctct
     1141 gcccaacttc ctcggaaagg gcgtggtcga agccaaagcg tcactgcagg cccttggcct
     1201 cacgatcgga aaaaccatca ccaagaagga cccatcacgc acgaaagaca cggtgatcat
     1261 gactcgaccg ggcccctacg acccggtcac agaaaatgcc accgtcgatc tcctggtttc
     1321 gtccggtctt gatgccggaa cagcgacggc tgaggacctg aagcgttttg aaaccgtcga
     1381 aggaccactg ccgtccaata cgcctccagc ccctcccacg atcaagatcg cggcatcggc
     1441 gacttcggtc gtcaaactgc cccctcagac c
//
LOCUS       DAORHD010001377         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_119979_length_1468_cov_4.902335, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001377 DAORHD010000000
VERSION     DAORHD010001377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119979_length_1468_cov_4.902335"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PKO06_21830"
     CDS             complement(<1..315)
                     /locus_tag="PKO06_21830"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNV72364.1"
                     /translation="MSQKRMIVVGAGNLGRRLAQALATSGFEVLVIDESEELNKQAQQ
                     QIEHSIDGEIARWGLTSGDKRAILARLQFSTKLSEAPDGGAVFEAISENLELKIALFK
                     QLD"
     gene            522..>1468
                     /locus_tag="PKO06_21835"
     CDS             522..>1468
                     /locus_tag="PKO06_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72365.1"
                     /translation="MRKEDLQLLLITKIPTNEKTQNEILETFESVCESRRMDEYLVLL
                     RAAYMNVPEIVTRRAFYVSLGEKLRKHLQQNPQLASGPTGLYLNFLAEDFQKTKESDL
                     IKVGRELETAMRLAANPDLRPVEGRRLADLAAVPMTPEDAFREPQTWIDKLAACELLF
                     KDEGIAPLLRELVAGNRKRARENANLLVLIGSHFEMASQPVWARACYRAAAGLDDTLE
                     LPWAFLAGLEPKLQDALACGNRAIRIHPRSLPGLRNRARVLARLNRPFEAFRDFAEVA
                     RCDSENPRALLDVAQSALDAGYALIAMRVYQFLSRRYPTL"
BASE COUNT          300 a          409 c          435 g          324 t
ORIGIN      
        1 atcgagctgc ttgaagaggg cgatcttgag ttcgaggttt tcactgatgg cctcgaaaac
       61 agcgcctcca tccggcgctt cgctcagttt ggtcgagaac tgcagacgcg cgagaatggc
      121 tcgcttatcg ccagaagtca ggccccaacg ggcaatttct ccgtcgatcg agtgttcgat
      181 ctgttgctgc gcctgcttgt tgagttcctc ggactcgtcg atgaccagga cttcgaagcc
      241 tgaagtggcc agggcttgcg ccagacggcg gcccagattc ccggcaccca cgacgatcat
      301 tcgtttttga ctcatggaat gtctcccgac cagtggtggt tgaatcagga atcgcgggtg
      361 taccccggaa attcccgtgc cgtattctac cgggcgacac tgcctcccgc aaggaaaatg
      421 tcgtgtgttg ccgttgtctg gaactctgag aggcaaacag gtagaatacc tgacagaaac
      481 tgatactcga atgctggctg aatacctgag gagggaccgc aatgcgcaag gaagatctgc
      541 agctgctgtt gatcacgaag atcccgacga atgaaaagac gcagaacgag attctcgaga
      601 cctttgaaag cgtctgtgag agccggcgta tggatgagta tctggttctg ttgcgtgccg
      661 cgtacatgaa tgtgcctgag attgtgacgc gtcgggcgtt ttacgtgtcc ctgggggaaa
      721 aactgcgaaa gcatctgcag cagaatcctc aattggccag tggcccaacc ggcttgtatc
      781 tcaactttct tgccgaggat tttcagaaaa ccaaagaatc ggatctgatc aaggtcgggc
      841 gagaactcga gaccgcgatg cggctggccg ccaaccctga tctgcgacca gtcgagggac
      901 gtcgtctggc agatctggcc gcggtcccca tgactcccga agacgccttt cgtgagccgc
      961 agacctggat cgacaaattg gctgcctgtg agctgctgtt caaagatgaa ggaatcgcgc
     1021 cgcttctgcg agaactggtt gccggcaacc gcaaacgggc gcgggaaaac gcgaacttgc
     1081 tggtgctgat cgggtctcac ttcgaaatgg cttcgcaacc ggtctgggcg cgtgcctgtt
     1141 atcgggcggc cgctggcctc gatgacaccc tggaactgcc gtgggctttt ctggccgggc
     1201 tcgagccgaa gttgcaggat gccctcgctt gtggcaaccg cgccatacgt atacatccgc
     1261 gcagtctgcc cgggttacgc aaccgcgctc gagttttggc gcgcttgaat cgtccctttg
     1321 aggcgtttcg cgacttcgcc gaagtggcac gctgcgattc ggaaaacccg cgagccctgc
     1381 tggatgtggc ccaatccgcc cttgatgccg gatatgctct gattgccatg cgagtgtacc
     1441 agttcctttc acgtcgatat cccacgct
//
LOCUS       DAORHD010001378         1468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_120006_length_1468_cov_3.150035, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001378 DAORHD010000000
VERSION     DAORHD010001378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120006_length_1468_cov_3.150035"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1468)
                     /locus_tag="PKO06_21840"
     CDS             complement(<1..>1468)
                     /locus_tag="PKO06_21840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV72366.1"
                     /translation="RTFQPLTVSVTTRLRFSLLHTNDLHSLVEGLGPDRCLDFSPGSA
                     DPVRGHYARLATLIRTSRRERESRSSSVFLVDAGDAEFGSLFHLIGPNPATEDMPEYR
                     FFHDLEYDLLGMGNHFFESGAHGLENAWAKVERGGWRLPFLVSNATFTDQAGLVARRF
                     VAQPLTQPTTGFHRLAIREVVADGQTLRIGFLGLVGPYAAKCSLANRSDLRFIGFDDD
                     SGRECPQELHAQARALVRELRDVHRVEVVVAIFHGGHPEDHALAKAVPEIDVIVAGHT
                     HAVYAETVGRTIVAQAGYGGTTLGCLDFVWQDGHLTWENPDASLLPVTSAVPADPDIL
                     ARVEAGRREVNRLLNGSSFAYETPVFALDHDLPRRRFPDNAAGVFAVSGILDELNRRI
                     IPAADLFVSSYGLVRSEFLCVAGRATVFQYSDIFKFFPLGFGRDLRAGTPVHVFSLTR
                     ADLKSLLEAMAALCLLFPVFEPVVSSSLSFRIAWWGIPF"
BASE COUNT          359 a          441 c          427 g          241 t
ORIGIN      
        1 agaacggaat tccccaccag gcgattcgaa atgacaacga cgaggagacg accggctcga
       61 acacgggaaa cagcaggcag agcgcggcca tcgcttcgag caggcttttc aggtccgctc
      121 gggtcaggga aaacacatgc accggggttc cggctcgcag atctctgccg aaccccagcg
      181 gaaagaactt gaagatgtcg gagtactgaa acacggtcgc acgaccggcg acgcagagaa
      241 actctgaacg tacaagtcca tagcttgaga caaatagatc tgctgcgggt atgatacgtc
      301 gattcagttc gtcgagaatg ccgctgaccg cgaatacacc ggccgcattg tccgggaaac
      361 gacgacgcgg cagatcgtga tcgagcgcaa acacgggagt ctcatatgcg aacgaagaac
      421 cgttcaacaa gcggttcact tctctccttc cggcctcgac ccgagccaga atgtccgggt
      481 cagcggggac cgcgctcgtt accggcagaa gcgaagcgtc ggggttttcc caggtcagat
      541 ggccgtcctg ccagacaaag tcgagacagc caagcgtcgt gccaccatac ccggcctgag
      601 ccacgatcgt gcggccgacg gtttcagcat acacggcatg ggtatgaccg gccacgatca
      661 catcgatttc cggaacagcc ttcgccagag cgtgatcttc ggggtgaccg ccatgaaaga
      721 tcgccaccac cacctcgacc cgatgcacgt cgcgcagctc acgtaccagt gctcgtgcct
      781 gcgcatgcag ttcctgagga cattcgcgac cagaatcatc atcaaacccg atgaatcgaa
      841 gatcagagcg gttcgccagc gaacacttcg ccgcgtaggg gccgacaagg ccgagaaaac
      901 cgatgcgcag agtctgaccg tccgcgacga cctcacgaat cgccagacga tgaaaccccg
      961 tggtcggttg ggtcaggggt tgagccacga aacgtctggc caccaaaccg gcctgatcgg
     1021 tgaacgtggc attcgacacc agaaatggca ggcgccaacc acctcgttcg accttggccc
     1081 aggcattctc gagcccgtgg gctcccgact cgaaaaagtg atttcccatt cccagcagat
     1141 cgtactcaag atcgtgaaag aatcgatact cgggcatgtc ttcggtagcg gggttcgggc
     1201 cgatcaaatg aaacaacgac ccgaattcgg catcaccggc gtcgaccaaa aacacgcttg
     1261 agctgcggga ttcgcgttcg cggcgactgg tacgtatcaa tgtcgccaat cgagcataat
     1321 gcccgcgaac cggatctgcg gagccgggag aaaaatccag acagcgatcg ggccccagac
     1381 cttcgaccag cgagtgcaga tcgttggtgt gcagcaggct gaatcgcagc cgggtggtga
     1441 cgctcacggt cagcggttga aaagtccg
//
LOCUS       DAORHD010001379         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_120396_length_1465_cov_3.427660, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001379 DAORHD010000000
VERSION     DAORHD010001379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120396_length_1465_cov_3.427660"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="PKO06_21845"
     CDS             complement(<1..1346)
                     /locus_tag="PKO06_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV72367.1"
                     /translation="MTRLASGWKARRWAVILAGGFVLFYQQSLTGWIVRSTYSWMHLE
                     YQVSFILIGLFVWTSPSWKRHLTILGWSAVPMVAAPLFIRLQAGEALFADRFAFATMH
                     PNLLASGFFLGFMALSIGLERTSDDRVWRLAGMTVLACGILLTQSRLQIVMLILGALY
                     SCNMLRPSRYRLLMLIMIGMCGIYFAGIGLHLVPDRFDYRNVPSERLFIWQAGMYRIL
                     NAPWGEGFLSFGHGLQPLTAAGHESVWDWFYPHTHSLYLETFLALGIPMGLAVMVFLL
                     VILLRRSSSEGGTILLGSQMVMGFAEFVWYTPFHLVMLIAAMAGEEEQASAETSDTRE
                     SPAPVPFLLLAFVALSLVGIQQERVERAAVLQQRGWQEMSAGHAAQALDAMQAAGDLD
                     PKRLEYPLYEALMSWQCGETENDRASAALRRLQRLLPDWDINWMAQAFQQVIQGND"
BASE COUNT          392 a          438 c          378 g          257 t
ORIGIN      
        1 tgatcattcc cctggatgac ctgttgaaag gcttgcgcca tccagttgat atcccaatcc
       61 ggcagaaggc gctgcaggcg acgcagcgcg gcgctggcac gatcattctc agtttcacca
      121 cactgccagc tcatcaatgc ctcgtagagt ggatactcca gacgctttgg atcgagatct
      181 ccggcagcct gcattgcatc gagcgcttgc gcggcatgcc cagcactcat ctcttgccag
      241 cccctctgct gcagaaccgc cgctcgttcg acgcgttcct gctgaatgcc gacaagagaa
      301 agcgcaacga acgccagaag aagaaagggc actggagctg gactttcacg cgtgtcactc
      361 gtctcagctg aagcctgttc ttcttcgccc gccattgcgg cgatcagcat caccagatga
      421 aacggcgtgt accagacgaa ctcggcgaat cccatgacca tctgtgagcc cagcagaatc
      481 gttccccctt cggaagaaga acgacgcaga aggatcacga gaagaaacac cataactgca
      541 agtcccatcg ggattcccaa ggcaagaaac gtttctagat aaagactgtg ggtgtgcggg
      601 taaaaccagt cccagacaga ttcatgacca gcagcggtta acggttgcag gccgtgtcca
      661 aagctgagaa aaccttcgcc ccacggcgca ttcaggatac gatacatccc cgcctgccag
      721 atgaataacc tctcgctggg aacattccga tagtcgaacc ggtcgggcac cagatgaagg
      781 ccgattccgg caaaatagat cccgcacatg ccgatcatga tgagcatcag aagtcgatac
      841 cgcgaaggcc tgagcatatt gcacgagtac agggcgccaa gaatcaacat cacgatctga
      901 agacgacttt gagtgaggag aatgccgcac gcaagaaccg tcattcctgc cagtcgccac
      961 acacgatcat cagaagtgcg ttcgagcccg atggacaagg ccatgaatcc gagaaagaat
     1021 cccgaagcta gcaggtttgg atgcatcgtg gcaaaggcaa aacgatcggc aaacagcgcc
     1081 tctcccgcct gcagacgaat gaacagtgga gcagcgacca tgggaacagc tgaccacccc
     1141 aagatggtca gatgccgttt ccagctcgga gaagtccaga caaagaggcc gatgagaatg
     1201 aagctgacct gatactccag atgcatccag gaatacgtcg aacgcacgat ccacccggtg
     1261 agactctgct ggtagaaaag aacaaaacct cccgcgagaa tcaccgccca acgccgcgct
     1321 ttccacccgg atgcgagcct cgtcatgact ctttccgcca gcaagagatt gaggattccg
     1381 gtttgccaac caaaactcat gccttgtccc agcgaaaccg cctgccaggg tatcatgcac
     1441 atgccgagtg cccagacagt ccagg
//
LOCUS       DAORHD010001380         1465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_120458_length_1465_cov_2.309929, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001380 DAORHD010000000
VERSION     DAORHD010001380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120458_length_1465_cov_2.309929"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKO06_21850"
     CDS             <1..729
                     /locus_tag="PKO06_21850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72368.1"
                     /translation="ASRLHGVSNHVRRAWLALAAKFRADPLTILLDLAIGITLLVLVF
                     LASSSFFSRPPDLSSLAKEWGFRTPGAVMAAQQLVEHSRHAPFPFPYERAYRALENPS
                     QIATDAGEGGLLFLFWINTQQQTAIEDRKAAAVLFLSRLTLHPELPTCGFAVLNSIKE
                     HWSSETIQSLRPHFRSFFLALHQTLSRSDSADKLHQFEALTNPNGDWQMIRPPDTPSP
                     SNPALEADSPEPASSSPETTERFE"
     gene            726..>1465
                     /locus_tag="PKO06_21855"
     CDS             726..>1465
                     /locus_tag="PKO06_21855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72369.1"
                     /translation="MRFSSKLLLFAILSLLLPAATQGASFLVTTQADFLAAGSSLSDV
                     LASTTEGLNADYLRIINTNPGLDDGFPAKKFTNGGTRVWNKKRAIQLEIPVDGTARAN
                     RYNEKVMVEISRIGGAKSDWADLRLTDFAGNPIDFGLVDFETPVDNTDSIVKLLFQAN
                     ATGTFASATYWIYYGNQDATNVATTSISRFYLTNHDFEDGMNGWTDLPAPLNAPVAAA
                     VAPVPASPLNRDTRLYTNIENDLSLVPPG"
BASE COUNT          323 a          467 c          359 g          316 t
ORIGIN      
        1 gccagccgac ttcacggcgt atcgaaccac gtcagaaggg catggctcgc cctcgccgca
       61 aagtttcgcg ccgaccccct caccatcctt ctcgatctgg cgatcggaat caccttgttg
      121 gtcctggttt ttctcgccag ttcatcattt ttctcacgcc cgccagactt gtcctctctg
      181 gcaaaggaat ggggatttcg cacaccgggt gcagtaatgg ctgctcaaca actcgtggag
      241 cactcgagac atgcaccctt ccccttcccg tatgaacggg cttatcgagc gctcgagaat
      301 ccgagccaga tagcaaccga tgcgggagaa ggaggcctgc tttttctgtt ctggatcaac
      361 acccaacaac agacggccat cgaagaccgt aaagcggcag cagtgttgtt tctttcgcga
      421 ctgacactgc accctgagct gccgacctgt ggcttcgccg tattgaattc gatcaaggaa
      481 cactggtcgt ctgaaaccat tcagtcactg cgtccacact ttcgctcatt ctttctcgcg
      541 ttgcatcaga ctctgtcacg aagcgacagc gctgacaagc tgcaccaatt cgaagccttg
      601 accaatccga acggggattg gcagatgata cgcccccccg acaccccgag cccgtcaaat
      661 ccggcactcg aggcagattc ccccgaaccc gcctcttcgt ccccagaaac cacggagcgt
      721 tttgaatgag attttcctcc aagcttcttc ttttcgcgat cctgtccctg ctgctgccag
      781 ctgccacaca aggcgcttcg tttctggtga caacgcaggc cgattttctt gccgccggct
      841 cctctctcag tgatgttctc gcgtcaacca ctgaaggact gaatgccgat tatctgcgaa
      901 tcattaacac caacccgggt ctcgatgatg gctttccggc aaagaagttc acgaacggtg
      961 gcaccagggt ctggaacaag aagcgcgcca tccagctcga aattcccgtt gacggcaccg
     1021 cacgcgccaa ccggtataac gagaaggtca tggttgaaat ctcgcgtatc ggaggggcga
     1081 aaagcgactg ggctgacctg cgattgaccg actttgccgg caacccgatc gatttcggcc
     1141 tggtggattt cgaaacgccg gtcgacaaca ccgattcgat cgtcaaactt ctgtttcaag
     1201 caaacgcgac tgggactttt gcctctgcca cctactggat ctactatgga aatcaagacg
     1261 ccacaaacgt cgcgacgacc agcatttcgc ggttctatct gaccaaccac gatttcgaag
     1321 acggaatgaa cggatggacc gatctgcctg ccccactcaa cgcccctgtc gccgcggcag
     1381 tcgcacctgt gccggcaagc cccctgaatc gggatacgcg cctgtatacg aacatcgaaa
     1441 acgacctgtc gctcgtgcca ccagg
//
LOCUS       DAORHD010001381         1463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_120655_length_1463_cov_3.654119, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001381 DAORHD010000000
VERSION     DAORHD010001381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120655_length_1463_cov_3.654119"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKO06_21860"
     CDS             <1..702
                     /locus_tag="PKO06_21860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV72370.1"
                     /translation="CHTHLAETRDEEEYCIKEYGCRPFELMEKLGWMDGNSFFAHSIH
                     LSPAEVKRMGKAKGGVAHCPSSNMRLGSGIAPIVEMKKAGVPVSLAVDGSASNDCSNM
                     MLEVRNCLLLQRVLKGASVITARDALTFGTLGGAQVIGRDDVGLIKVGYEADLAGFRL
                     DGLRQAGSSTDPLAALVFCAVDTTDLVVCHGELLRHQGQFTRFSRDEIAELTRRQNER
                     SRQIYASAKKPGRKK"
     gene            826..>1463
                     /locus_tag="PKO06_21865"
     CDS             826..>1463
                     /locus_tag="PKO06_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HNV72371.1"
                     /translation="MSQEFSLISLEHLLRWVLREEETGEIFGIPRELFFKPSLDDPFR
                     VERYGQTLESPIGVAAGPHTQMAQNIIAAWLCGGRYIELKTVQTLDELKVSKPCIDLG
                     DEGYNCEWSQELRLKDSFNEYLHAWIILHILRHKWGWANDASPGFLFNLSVGYNMEGI
                     LKPNVQEFFRKARSCKELLAEKIESIKHLYPDVTRLAIPDRISDNITLSTMHG"
BASE COUNT          318 a          480 c          397 g          268 t
ORIGIN      
        1 tgccataccc atctggccga aacccgtgac gaagaagagt attgcatcaa ggagtacggc
       61 tgccgcccct tcgaactcat ggaaaaactg ggctggatgg acggcaactc gttcttcgcc
      121 cactcgattc acctgtcgcc cgccgaggtg aaacgcatgg gcaaggccaa gggcggcgtc
      181 gcccactgcc cgagcagcaa catgcggctc ggctcaggca tcgccccgat cgtcgaaatg
      241 aaaaaagccg gtgtaccggt cagtctcgcc gtcgacggtt ctgccagcaa tgactgctcg
      301 aacatgatgc tcgaagtgcg caactgtctg ctgctgcagc gggtgctcaa aggcgcttcc
      361 gtcatcaccg cccgagatgc gctgacgttc ggcaccctcg gcggcgctca ggtgatcgga
      421 cgcgacgatg tcggtctgat caaagtcgga tacgaagccg acctcgccgg gttccgcctg
      481 gatggccttc gccaggccgg cagttcgacc gacccgctgg ctgcgctggt cttctgcgcc
      541 gtcgacacca ccgacctcgt ggtctgccac ggtgaactcc tgcgccacca gggccagttc
      601 acccgcttca gccgcgatga gattgccgag ctgacccgcc gccagaacga acgttcacgc
      661 cagatctacg cctcggcgaa aaagcccggt cgcaaaaagt aacccccgcg ctccgtcgcc
      721 gcgacgacac gacttttgtc cgagttgtcg cggtgtacag agcacatttt atcaagcaaa
      781 gagaccgcga cgtacattgt cgtcgcacga tcaaggagaa acggaatgtc ccaggaattt
      841 tccctcattt ccctcgaaca cctgctgcgc tgggttttgc gcgaagaaga aacgggcgag
      901 atcttcggta ttccccgtga actgttcttc aagccatccc tcgatgaccc cttccgcgtc
      961 gaacgctacg gccagacgct cgaaagcccc atcggcgtcg cggccggccc gcacacacag
     1021 atggcgcaga acatcatcgc cgcctggctc tgcggtggcc ggtatatcga actgaagacc
     1081 gtgcaaacgc ttgatgaact caaagtctcg aagccctgca tcgatctcgg cgacgaaggc
     1141 tacaactgcg aatggtcgca ggaactgcgc ctgaaagact cattcaatga gtatctgcac
     1201 gcctggatca tcctgcacat tctgcgtcac aaatggggct gggccaacga tgccagcccc
     1261 ggcttcctct tcaacctcag cgtcgggtac aacatggaag gcattctcaa gcccaacgtg
     1321 caggaattct ttcgcaaagc ccgttcctgc aaagagctgc tggccgagaa gatcgaatcg
     1381 atcaagcacc tgtaccccga tgtgaccagg ctcgccattc ccgaccgcat ctccgacaac
     1441 atcacgctgt cgaccatgca cgg
//
LOCUS       DAORHD010001382         1460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121047_length_1460_cov_3.305338, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001382 DAORHD010000000
VERSION     DAORHD010001382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121047_length_1460_cov_3.305338"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKO06_21870"
     CDS             <1..948
                     /locus_tag="PKO06_21870"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNV72372.1"
                     /translation="CRLEGLVSPDWHKLDNFIRRSIPLGMTSRRDALVFRQKSILAAE
                     SDRWLELCQVMERQFFAEYRIGRHTSPELQLATLGGGNHFIEVDRGTNGDLYLLIHSG
                     SRNFGLRVAEHYQKRARDYTSRRQLAVPHGTEFLPIDGGGDEYLHWAQQAQLYARYNR
                     RAMMMLILDFLGVPLRESNVFESVHNYISPIDQIVRKGAISAREDERVVIPLNMADGT
                     IIGRGRSNAAYNFSAPHGAGRVHGRQEMKRMLASGTLTMQQYHASMSHVYSTSVSRQT
                     IDESKFAYKPFDLIRDHLEATVQIEDHLTPVYNLKASED"
     gene            complement(1186..>1460)
                     /locus_tag="PKO06_21875"
     CDS             complement(1186..>1460)
                     /locus_tag="PKO06_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72373.1"
                     /translation="PPRPATAADRASTTTTNPTKSSKGYLKVTDSKKTKAKPATKTTS
                     PKKTTTSKSDQKPAGTDKTATPNPAPAEPPTSPDLPAIGDAGRFEM"
BASE COUNT          294 a          399 c          433 g          334 t
ORIGIN      
        1 tgtcgtctcg aggggctggt atcacccgac tggcacaagc tcgacaactt catccggcgg
       61 tcgattccac tgggaatgac gtcgcgacgc gatgctctcg tctttcgtca gaagtcgata
      121 ttggcggcag aatccgatcg ctggctcgaa ctgtgccagg tcatggagcg tcaattcttc
      181 gccgagtatc gcatcggccg tcacacatct ccggaattgc agctggcgac gctgggcggt
      241 ggcaatcact tcatcgaggt tgatcgtggc acgaatggag atttgtacct gttgattcat
      301 tccggatcac gcaattttgg cctgcgagta gccgaacact atcagaaacg ggcgcgagac
      361 tataccagtc gtcgccagct cgccgttccg catggcaccg aatttctgcc aatagacggt
      421 ggaggagacg aatacctgca ttgggcacag caggcgcagt tgtatgcccg gtacaatcgc
      481 cgggccatga tgatgctgat actcgacttt ctcggggtgc cgcttcgtga aagcaacgtc
      541 ttcgagagcg ttcacaacta tatttcaccg atcgatcaga ttgtacgaaa gggggccatc
      601 tcggcccgcg aggacgagag ggtcgttatt cccctgaaca tggctgatgg caccatcatc
      661 gggcgcggtc gttcgaacgc cgcctacaac ttttccgccc ctcatggagc cggtcgcgtg
      721 cacgggcgcc aggaaatgaa gcgcatgctt gcatccggga cgctcacaat gcagcagtat
      781 catgcttcga tgagtcacgt ctattccacc tcggtttccc gtcagaccat tgatgagagc
      841 aagtttgcct acaaaccttt cgacctgatc cgcgaccacc tcgaagccac cgtccagatc
      901 gaagaccatc tcaccccggt ctacaacctg aaagcatcag aagattgatc tttgtgccgc
      961 gagctgtcgg tgctcaaagc cggggagcgg tctgaggcga gcgagtcgaa acgcccgagc
     1021 ccacgcagca aaaagagact tttgccctcg tcgcgaaagg cattgacgcg atcacgatgg
     1081 cgagtcacag tatcctcttt ctgaagtcac gacatggtca gaaaagagaa ccgggtcggt
     1141 tccgcctgag cggagccgac ccgggaaacg gaccggagac tgtttttaca tctcgaagcg
     1201 acccgcgtca ccgatagccg gcagatcggg agaggttggc ggttctgcag gagcgggatt
     1261 cggggttgct gttttgtctg taccagctgg cttttgatct gacttgctgg tggtggtctt
     1321 tttgggtgag gtcgttttgg ttgctggctt tgctttcgtc ttcttggagt cggtgacctt
     1381 caggtatccc tttgaggact tggttggatt tgtcgtggtg gtcgacgcgc gatcggccgc
     1441 cgtggcaggt cgcggaggct
//
LOCUS       DAORHD010001383         1459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121220_length_1459_cov_2.664530, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001383 DAORHD010000000
VERSION     DAORHD010001383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121220_length_1459_cov_2.664530"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKO06_21880"
     CDS             complement(<1..185)
                     /locus_tag="PKO06_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72374.1"
                     /translation="MNRKLISLILLAVIFVASGGCGGGGGGSGRYTLPGEIPTPGDDG
                     GGGGGGGTGTTDPNALLT"
     gene            complement(213..>1459)
                     /locus_tag="PKO06_21885"
     CDS             complement(213..>1459)
                     /locus_tag="PKO06_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72375.1"
                     /translation="QAVVLSAGPTTRLYLGGAHLDKNFPGVATIKVTAKDVSGNSGVT
                     TVSFSTAFINASVRAEVRSADQKFAAIFPAGSLKQNSLITVVPTGLTKIPAAANRKAA
                     VRPSILANVSGGKSASLVAALGEGIAGDELVPVAQAYMVNIPTGRLQGNLEVAMQAGG
                     SLPTGTALFRQVDGKWVFASRTVSDSQLRAVVDRAGLFALLRDEKAPRANLLTKVDAD
                     KPLTASRPTFEWAVAEDGAGLDLPGCKARLNGVEMPVTYDATTGKVLFVPENGLISGQ
                     YRLALEVRDLAGNVNVLPEIRFNLDPALQIFEITQYPNPASRRATIRISTNRQDIDAS
                     HIDVSIYDINGDRVASGRSLFVNSTNFGQNGRVVHDVQWDLVNDNGRRVANGIYFAKI
                     TLHDPDNWEKKSRVTHKIAVLR"
BASE COUNT          306 a          459 c          392 g          302 t
ORIGIN      
        1 gtcagcagtg cattcgggtc cgtcgttccc gttccacccc cgccaccgcc accgccatca
       61 tcaccagggg ttggaatctc accgggcaga gtgtagcggc cactgccacc accaccgcca
      121 ccacagccgc ctgaggcaac gaagatcact gcgagcagaa tcagactgat cagtttccgg
      181 ttcatagtgt gttccttttt gtaccggttg tgttaccgca ataccgcgat cttatgggta
      241 acccgagact tcttctccca gttgtccggg tcatgcaacg tgattttcgc gaagtagatg
      301 ccgttcgcca cccggcgccc gttgtcattc acgagatccc actgcacgtc atgaaccaca
      361 cgcccgttct gtccgaagtt cgtgctgttc acaaacagac tgcgaccgct ggcaacgcga
      421 tcaccattga tgtcgtagat cgagacatca atgtgcgagg catcgatatc ctgacgattc
      481 gtcgagattc gaatggtcgc gcgacgcgaa gccgggttcg gatactgggt aatctcaaag
      541 atctgtaatg ccgggtcaag attgaagcga atttccggca gaacattcac gtttccagcc
      601 agatcgcgca cttcgagcgc gagacgatac tgaccgctga tcaagccgtt ctcgggcaca
      661 aagagcactt ttcccgtcgt ggcgtcatac gttaccggca tctcgacccc attcagtcga
      721 gccttgcagc ccggcaaatc aagaccagca ccgtcttcgg ctacggccca ttcgaacgtc
      781 ggccgcgagg ccgtcaacgg tttgtccgca tcgactttgg tcaggaggtt cgcccgaggc
      841 gccttttcgt cacgcagcag cgcaaacaga ccggcccggt cgacaacggc ccgcaactga
      901 ctgtcgctca ccgtgcggct ggcgaagacc cacttgccat caacctgacg gaacaaggcc
      961 gttcctgtgg gcaaagaacc acccgcctgc atggctacct caagatttcc ctgcaagcgc
     1021 ccggtcggaa tgttaaccat gtatgcctga gcgactggta ccagttcgtc accggcaata
     1081 ccttcaccca gtgccgcaac caatgatgcg gattttccgc cgctgacgtt ggccagaata
     1141 ctgggtctaa cagcagcctt gcgattggcg gcggctggta tcttggtcaa tccggtcgga
     1201 accacggtga tcagcgagtt ctgtttcagt gaaccagcgg ggaagatcgc cgcgaacttc
     1261 tggtccgccg agcgcacttc tgcccgcact gaggcattga tgaacgcggt cgagaagctg
     1321 accgtcgtaa cacctgagtt tccagacaca tccttggcag tcaccttgat ggtcgccacg
     1381 ccagggaagt tcttatcgag atgtgcgcca cccaggtaga gtcgcgtcgt cgggccggca
     1441 ctcagcacca cggcctgag
//
LOCUS       DAORHD010001384         1457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121498_length_1457_cov_2.672611, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001384 DAORHD010000000
VERSION     DAORHD010001384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121498_length_1457_cov_2.672611"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKO06_21890"
     CDS             <1..569
                     /locus_tag="PKO06_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72376.1"
                     /translation="QARIAERVAAGKLPLFLLGGKAATSVSTATPMKAATSEPSGKTT
                     ILYSEHDNVSLAAANKTPENHFRPDEVDILTKELGSDLPYIETALGSLSTTGHDSLQL
                     DSPQVAGDAELLQTGIQEPADALGRLSESTEPATTRELETGSLVKRSPNNSDLELLNI
                     DVPSSSHETGDLLPMLRLDDSSLMPDRD"
     gene            complement(640..996)
                     /locus_tag="PKO06_21895"
     CDS             complement(640..996)
                     /locus_tag="PKO06_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72377.1"
                     /translation="MKVELAKAVYQLSPGTSKQTVEQAVARSAQEVNRVESARLKTDN
                     ASTKVEDAYLSVRNKRSSLHSATYQYRVQETRKATEQISGAVASTQRDIVKVLSTIIS
                     PNVAPENLGRFIDRFS"
     gene            1157..>1457
                     /locus_tag="PKO06_21900"
     CDS             1157..>1457
                     /locus_tag="PKO06_21900"
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNV72378.1"
                     /translation="MGFPVHRPRRLRQSPAMRNLVRETCFQAKHLVLPLFIEEGLSNP
                     EPIASMPGQFRWPWQEVGSIVDQATNAGIHAFLLFGSPAEKDMTGSSSRDADGVIP"
BASE COUNT          310 a          444 c          379 g          324 t
ORIGIN      
        1 ctcaggcacg gatcgccgag cgggttgccg cgggcaaact cccgttgttt cttctcggcg
       61 gcaaggcggc aacctctgtc tctacggcca cacccatgaa ggcggcaacc tctgagccgt
      121 caggcaaaac gactattctg tattcggaac acgacaatgt cagcctagct gccgccaaca
      181 aaactcctga aaatcatttc cgccccgacg aagtcgatat tctcaccaaa gaactgggga
      241 gcgatctgcc ttacatcgaa accgcgctcg gcagtctctc cacgactggt cacgactcgc
      301 tgcagctcga ctccccgcag gtagccggtg atgctgagct gcttcagacc ggtattcagg
      361 aaccagccga cgcactcggt cgtttgagcg agtcaaccga acccgcgaca acgcgagaac
      421 tcgaaaccgg gtctctcgtg aaacgatcgc ccaacaactc tgatctcgag ctgttgaaca
      481 tcgatgtccc ctccagcagt cacgaaaccg gcgatctgct ccccatgctg cggcttgacg
      541 attcatcact catgcccgat cgcgactgag aggcccgcaa agtggcctga aaacacagaa
      601 cccagcctga acggctgggt tctgtgtttt tcggaaccat caggaaaagc gatcgatgaa
      661 tctgccgagg ttttcggggg caacgttcgg ggaaatgatg gtgctgagca ctttgacgat
      721 atcgcgttga gttgatgcca cagcgcctga gatctgttcc gtggccttcc gcgtttcctg
      781 cacccgatac tgataggtcg cggaatgaag tgatgaacgt ttgtttctga ccgacagata
      841 ggcgtcttcg accttcgtac ttgcgttatc ggtcttcaga cgcgcggatt caacgcggtt
      901 gacctcctgg gccgaacgcg caacagcctg ctccacggtt tgctttgacg tcccgggaga
      961 aagctgatag accgctttag cgagttcaac cttcatttgt ttgctccgga cacacgaccc
     1021 ctaatctaac tcgttgtact ctaaatatac gcaacagctg ctctgtgtca aacttctggt
     1081 gttccgctgg ttgcgggtgt gcaggcatag tgttacactt cctcctgctt tcatcgtcac
     1141 aaccagcgga ggtcgcatgg gattccccgt tcatcgccct cgacgcctga gacaatcgcc
     1201 ggccatgcgc aatctggtcc gggaaacctg ctttcaagcc aagcatctgg tacttccgct
     1261 gttcatcgaa gaggggttgt cgaaccccga gccgatcgct tccatgcccg gtcagtttcg
     1321 ctggccctgg caagaagtcg gcagcatcgt cgatcaggca accaacgcag gaatccatgc
     1381 tttcctgctc ttcggttctc ccgccgaaaa ggatatgacg ggctcatcat cacgcgatgc
     1441 tgacggcgtc attccgc
//
LOCUS       DAORHD010001385         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121606_length_1456_cov_3.602427, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001385 DAORHD010000000
VERSION     DAORHD010001385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121606_length_1456_cov_3.602427"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKO06_21905"
     CDS             <1..390
                     /locus_tag="PKO06_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HNV72379.1"
                     /translation="LIAFAKANPGKLNYASGGSGTSAHLGAELFKSMAGVFITHVFLG
                     QAHIERGVKNPTRFVTKESVQFRCQIDGNGRMQDRRGWCHIKHDPVDQLAPHLFRPGA
                     VIGVGKLFRRGRFSFCLWHTDFSVRKG"
     gene            696..>1456
                     /locus_tag="PKO06_21910"
     CDS             696..>1456
                     /locus_tag="PKO06_21910"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNV72380.1"
                     /translation="MKSHDASYKKFFSFPRMVEDLLRGFVHEEWVKKLDYSTLERVNG
                     SYVADGFKQRHDDVVWRVRWGDDWLYVYILIEFQSDVDPFMALRIMTYLGLLYQDLVN
                     QKLLYQGRLLPPVLPIVLYNGNPRWNASVDVQELIVPMKGGLERYCPRLSYLLIDEGA
                     YAAGELSELQNLVAALFRLEKSRDLETVRHAIKNLIEWLKMPEQAGLRRAFTRWIYHV
                     FIKTRTPQGAIPEVTDLEEAQSMLSKTVVEWTKQW"
BASE COUNT          317 a          391 c          409 g          339 t
ORIGIN      
        1 ctgatcgcct ttgccaaggc caatccgggc aagctgaact acgcctcggg aggatcgggc
       61 acatcggcgc acctgggggc cgagctgttc aagtcgatgg ccggtgtctt catcacgcac
      121 gtttttcttg ggcaagcgca catcgaacgg ggcgtcaaaa acccgacacg ttttgtcacg
      181 aaagaaagtg ttcaattccg ttgccagatt gatggaaatg gtcgcatgca ggacagacgg
      241 ggctggtgtc atatcaaaca cgaccccgtc gatcagctcg caccgcacct cttccggcca
      301 ggtgcagtaa tcggcgtagg taaacttttt cgacgcggca ggttttcgtt ttgcctgtgg
      361 cataccgatt tctccgtcag aaaagggtga cgacctcggc cattctaaca caaggccagg
      421 acgggaagaa gtcatgggaa agagttcccg tgtgccagag tcgagggttg tatccccgcc
      481 attccttatc ggcgtgatgc aggtctgccg aattacgtat actgttcccc gagctccccg
      541 atctcttaca atctctgatc ttgctccaat ctgatcgccc gatctgacac atacggtctt
      601 cccgttcccc gggcctctcc ttcccccaac ggtgcgatct gtcgttgaga cagctgtctt
      661 gcctgcattc gggaacccgg ggtagaatga gcgtgatgaa aagccacgac gcgagctaca
      721 agaagttctt ctcgtttcct cgaatggtgg aagatctcct cagagggttc gtccacgagg
      781 agtgggtgaa aaaactcgac tattccactc tcgaacgagt gaacggaagt tacgttgccg
      841 acggtttcaa acaacgtcat gacgacgtag tctggcgcgt gcgctggggc gatgactggc
      901 tgtatgtcta tattctgatc gagtttcagt cagacgtcga tccattcatg gccctgcgca
      961 tcatgacgta tcttggcctg ctctatcagg atctggtcaa ccagaagctt ctgtatcaag
     1021 gtcgactcct gccaccggtt ctgccgatcg ttctttacaa cgggaacccg cgctggaacg
     1081 cgtccgtcga tgtgcaggag ttgattgtac caatgaaggg cggcttggag cgctattgtc
     1141 ccaggctttc gtatctgttg attgacgagg gagcgtatgc ggcaggggaa ctgtccgagt
     1201 tgcagaatct ggttgccgcc ttgtttcggc ttgagaagtc acgcgatctc gaaaccgtca
     1261 ggcacgcgat taaaaatctg atcgagtggt tgaagatgcc cgagcaggcg ggcttgcgta
     1321 gagcctttac ccgctggatc tatcacgtat tcatcaagac ccggaccccg cagggggcaa
     1381 tacctgaggt cactgacctc gaggaggcgc agagcatgtt atcgaaaacc gtcgtcgagt
     1441 ggaccaaaca atggaa
//
LOCUS       DAORHD010001386         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121902_length_1454_cov_3.486061, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001386 DAORHD010000000
VERSION     DAORHD010001386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121902_length_1454_cov_3.486061"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PKO06_21915"
     CDS             <1..1067
                     /locus_tag="PKO06_21915"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HNV72381.1"
                     /translation="ASTFKFPLTDLRAPFVLSEGVLAINGFKARSFDGALEGSGKIFL
                     KETPLRFAFDTKATQLMVQNFLALNTSLKNALTGGLDFQLKMQGNTTGLDSVQGESDI
                     HMKNGKYQAPPVAAQIFEVLQAPQLASGDITGAQGHFVIKDGRMNSNDLLFRSRYGSL
                     GYAGTVGLDTSLDGTAKIELTQEACQMSTTLRQLAGSKKSLTLPASVKGSLLSPKVGL
                     KIEDLLKEAAKQKLQDTLLDAVMKGGKKEPSASGSSTIASESASPATPQDLLMQQLGK
                     KIGIKLPGQTTTAPAPTPAPPAPAATSTTPVSVASGAVSIPPASGTAVPAPETKPTKV
                     KDVGNEIKKIGKDLKKIFKW"
     gene            1093..1452
                     /locus_tag="PKO06_21920"
     CDS             1093..1452
                     /locus_tag="PKO06_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72382.1"
                     /translation="MTDKKDFDVRFELSGKVAKIFVQGPLKEEAGLDLRSIVERKIEE
                     GFTRFVFDFGNSRAMSSPAVATVLDLAEMIVDSKSGKLIISGLTDLNMKVFEMVGIFM
                     YAEACATHMEAEVRALM"
BASE COUNT          350 a          374 c          432 g          298 t
ORIGIN      
        1 gtgcatcgac attcaagttt cctctgactg atctgcgcgc gccgtttgtg ctgtcggaag
       61 gcgtgctggc gatcaacggc ttcaaagctc gcagttttga cggggctctc gaagggtccg
      121 gtaagatctt tctgaaagag acaccgttgc gcttcgcgtt cgacaccaag gccacacagc
      181 tgatggtgca aaactttctg gcgctcaata ccagcctgaa gaatgcgttg accggcggtc
      241 ttgattttca gttgaagatg cagggcaaca cgactggtct cgattccgtg cagggcgagt
      301 cggacattca catgaaaaac ggcaagtatc aggctcctcc ggtcgcggcg cagatcttcg
      361 aggtgttgca ggcgccgcaa ttggcttccg gcgacattac cggggctcag gggcatttcg
      421 tgatcaagga cggcaggatg aactcgaacg acctgctgtt ccgcagtcgg tatggaagcc
      481 tcgggtatgc aggaaccgtg ggtctcgaca cgtccctcga cggaacagcg aagatcgaac
      541 ttacgcagga agcgtgtcag atgagcacga ctttgcgaca gctggcgggg agcaagaaga
      601 gtctcacgct gcctgcgtcg gtcaaaggca gcctgctgtc tccgaaagtt ggattgaaaa
      661 tcgaggatct gctcaaggaa gccgcgaagc agaaactgca ggataccttg ctggatgccg
      721 tgatgaaggg cgggaagaag gaaccgtcgg catcgggttc ctcgacgatc gcctccgaga
      781 gcgcgtctcc ggccacgccg caagacctgc tgatgcagca gctgggaaag aaaattggta
      841 tcaagttgcc cgggcagacg acgactgcgc cggcaccgac acctgctcca cccgcacccg
      901 cggcgacgag tacgacaccc gtttccgttg cctctggtgc tgtctcgata cccccggcca
      961 gcggaacggc tgttcctgca cctgaaacga aaccgacgaa ggtgaaggat gtggggaacg
     1021 agatcaagaa aataggaaaa gatctgaaaa aaatattcaa atggtgagca tatctcgtag
     1081 tagactaacg atatgacaga caagaaagat ttcgacgtac gatttgagct ttctggaaag
     1141 gttgccaaga tcttcgttca aggccctctc aaagaggaag ccggtctcga tctgcgttcc
     1201 atcgtcgaac gaaaaatcga agaagggttc actcgatttg tctttgactt cggcaattcg
     1261 cgcgccatga gcagccctgc ggtggcgacg gtcctcgatc ttgccgagat gatcgttgat
     1321 tcaaagagtg gcaagttgat catttccgga ctgaccgacc tcaacatgaa ggtcttcgaa
     1381 atggttggca tcttcatgta tgccgaagcg tgcgcaaccc acatggaggc ggaagtcagg
     1441 gccttgatgt aggg
//
LOCUS       DAORHD010001387         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121910_length_1454_cov_3.335954, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001387 DAORHD010000000
VERSION     DAORHD010001387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121910_length_1454_cov_3.335954"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1334)
                     /locus_tag="PKO06_21925"
     CDS             complement(18..1334)
                     /locus_tag="PKO06_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72383.1"
                     /translation="MIKTNRCFVGLLGLVSMLISACVVTAAPAYRAERRPLAGFIKAF
                     QDISEETLGIVRNCNTDFETAEASPDEVTARVAAGKAFYEACRERFEKLYAEADKILA
                     SPPQKIPQDRMPQTSLDTPTFDHRFNRELARCFVLTALYHSGAGGAEQAVKTLLLMNW
                     FGHAISRGMGEVPTLIDLMIGIAIRKMTCGEVLWEALAKCGFSGERLDAWTKALNSLD
                     ASQPSYVDSLECELMGTLNATRKALQGTSNNDQMVRLMQHLPAGNREKILGPAMEYLQ
                     AVNERLLNIASVYRASPRKMREATIAFVETLESAMVMRLSDYLNPGAKIGQILASIMI
                     PNFARAYEQYLLARYRERGTVLLMQYRARLGKGTALPKTAADFEAVTKSRIPADVFVE
                     ARTACRYVLNEKWMMVYSLGPDQKDDQGHPVNDGLLFKVPVRLLRP"
BASE COUNT          280 a          452 c          404 g          318 t
ORIGIN      
        1 gccgacgggg agtaaattca gggtcgcagc aggcggaccg ggaccttgaa gagcaagccg
       61 tcgttcaccg gatggccctg atcgtctttc tgatcggggc caagtgagta aaccatcatc
      121 catttctcgt tgagcacata tcgacaggcg gttcgggctt cgacgaacac gtctgccgga
      181 atccggcttt tggtcacggc ttcgaagtcc gccgcggtct tcggcagagc ggttcccttg
      241 cccagacggg cgcgatactg catcaacagc acggttcccc gttcacgata gcgggccagc
      301 agatactgtt cgtacgccct ggcgaagttg ggaatcatga tcgatgccag aatctggccg
      361 atcttcgcgc ccggattcag gtaatcagac agacgcatga ccatggcact ttcgagcgtt
      421 tcgacaaagg caatggtcgc ttcgcgcatt ttgcgcgggc tcgcgcgata caccgaagcg
      481 atgttcagca gtcgctcgtt gactgcctgc aaatactcca tcgccggtcc gagaatcttc
      541 tcgcgattcc ccgccggcag gtgctgcatc aaccgcacca tctgatcatt gttgctggtc
      601 ccctgcaaag ccttccgggt ggcattgagc gttcccatca actcgcactc gagggaatcg
      661 acatacgagg gctgtgacgc atccaggctg ttgagagctt tggtccacgc atccagacgc
      721 tcgccgctga aaccgcactt tgccagggcc tcccagagca cctcaccaca cgtcatcttg
      781 cgaatggcga tcccgatcat cagatcgatc agcgtcggca cctcccccat gccgcgagat
      841 atcgcgtgcc cgaaccagtt catcagaagc agcgtcttca cggcctgctc ggcaccgccg
      901 gcgccgctgt ggtacaaggc cgtgagtacg aaacatcggg ccagttctcg attgaaacga
      961 tgatcgaatg tcggagtgtc gagactggtc tgcggcatgc gatcctgagg aatcttctgg
     1021 ggcgggctcg cgagaatctt gtcggcttct gcgtagagtt tttcgaaacg ctcgcggcag
     1081 gcttcgtaga aggccttccc ggccgccacc cgcgccgtga cttcgtcagg actggcttcc
     1141 gccgtctcga aatccgtgtt gcagtttctc acgatcccga gcgtttcttc cgaaatatcc
     1201 tgaaacgctt tgataaagcc ggcgagcggt cgacgttccg cccgataggc cggggccgcc
     1261 gtcacgacgc aggcgctgat cagcatcgac accaggccca gaagtcccac gaagcatcgg
     1321 tttgtcttta tcattgccga gttccttttc ctgtagcctc tctccccatg tttctcccgg
     1381 tacttttcac ggcagcctcc cgtgtcggga acagcgaatg aaggcattgt caccagttcc
     1441 ttccatggtg tact
//
LOCUS       DAORHD010001388         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121914_length_1454_cov_3.257327, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001388 DAORHD010000000
VERSION     DAORHD010001388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121914_length_1454_cov_3.257327"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PKO06_21930"
     CDS             <1..618
                     /locus_tag="PKO06_21930"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV72384.1"
                     /translation="IRETLQKAQDYLEQKNSKKGLRDRSLQMEALCGLLEKKVPMWAH
                     ADRLEDIMSAIRIAEEFDLRLVIEHGTEAHLAAKLLAEKKIPVVLSNTVMHPIRFDVV
                     GHAYEHAVELLDAGVEVAFASDAPHLPADNLRLTAAMAIQYGLDEKRAIQCITEFPAK
                     ILGVANRIGQVKKGYDGDVALFSGHPLDMRSRMEILVLDGEVFRF"
     gene            complement(686..1054)
                     /gene="queD"
                     /locus_tag="PKO06_21935"
     CDS             complement(686..1054)
                     /gene="queD"
                     /locus_tag="PKO06_21935"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNV72385.1"
                     /translation="MYRITIEKSISSAHCLRQYEGKCRELHGHNWNFQVTIEADQLDS
                     LGMVLDFGVVKRHLADLLEPFDHKLLNDVPPFDRLNPTAENISSFVFDELSKRLNTDR
                     VKVMSVKTWETPTNVAEYHR"
     gene            complement(1056..1397)
                     /locus_tag="PKO06_21940"
     CDS             complement(1056..1397)
                     /locus_tag="PKO06_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72386.1"
                     /translation="MTGIEFLRDLKDYFGPRSMRSLSFQIEQMKRSLVFLGRGHGHGI
                     GLCQWGSDGMAKSGQTYREILEFYYKGAAVNRIHQWQENTRIGTTANVPFTVPPGSAS
                     RLIASPSSEVR"
BASE COUNT          332 a          372 c          408 g          342 t
ORIGIN      
        1 attcgcgaaa ccctgcagaa agctcaggat tacctcgaac agaagaactc gaaaaagggg
       61 ttgcgtgacc gaagcctgca aatggaagcg ctctgcggcc ttctcgaaaa gaaggttccg
      121 atgtgggcac acgccgatcg tctcgaagac atcatgtcgg cgatccgtat cgcggaagaa
      181 ttcgatctga gacttgtcat cgaacatgga actgaagcgc atcttgcggc aaaactgctc
      241 gcagagaaaa agataccggt cgttctcagc aacacggtca tgcacccgat tcgtttcgat
      301 gtggtcggac atgcctacga acatgctgtc gagctcctcg atgcaggagt ggaagtcgcg
      361 tttgccagtg atgctccgca tctgccggca gataatctgc gcctgacggc ggcgatggcg
      421 attcagtatg gtcttgatga gaaacgcgca attcagtgca ttacggagtt tccggcgaag
      481 atcctgggtg tcgccaaccg tatcggacaa gtgaaaaaag ggtatgacgg agatgtcgcg
      541 ctcttctcag gtcacccgct cgacatgcgg tctcggatgg aaatcctcgt tctcgacgga
      601 gaagtttttc gcttctaaga gtccatcagt taaagaacca cccaagcccc ggcctgggtg
      661 gttcttttct ttcaagcgaa tggcactaac ggtggtattc ggctacgttg gtcggcgtct
      721 cccaggtctt gaccgacatt acttttaccc ggtcggtgtt gaggcgtttt gataactcgt
      781 caaaaacgaa tgaactgatg ttttcggctg tcgggttcag gcggtcaaac gggggaacat
      841 cattcaaaag cttgtgatcg aacggttcga gcaggtctgc gaggtggcgt tttaccacgc
      901 cgaagtcgag gaccattccc aaactgtcga gttgatcggc ttcgatcgtg acctggaagt
      961 tccagttgtg gccatgcagt tcgcgacatt ttccttcata ctggcgcaga caatgtgctg
     1021 acgagatgct cttctcgatt gtgattcggt acatttcaac gcacctcaga actcgggctt
     1081 gcgatcagcc tggaggcaga gccaggggga accgtgaaag ggacgtttgc cgtggttccg
     1141 atgcgcgtgt tttcctgcca ttgatggatg cggttgaccg ccgctccctt gtaatagaat
     1201 tcgagaatct cgcgatacgt ctgtccagac ttggccatgc cgtcagaacc ccattgacag
     1261 aggccgattc catggccgtg ccctcggccg agaaagacca gagagcgctt catctgttcg
     1321 atctgaaagc tcaggctgcg catggagcga ggaccgaagt aatccttgag gtctcgaagg
     1381 aactcgatac ccgtcacgac cttttcacga cgtcaactta attttacgct gagaatgcga
     1441 ccgttcgcgt cgtg
//
LOCUS       DAORHD010001389         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_121933_length_1454_cov_2.995711, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001389 DAORHD010000000
VERSION     DAORHD010001389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121933_length_1454_cov_2.995711"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PKO06_21945"
     CDS             complement(<1..571)
                     /locus_tag="PKO06_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72387.1"
                     /translation="MSGPMRSFLVLNGAVLVFLAIVWWADGQIPFDRIKASADHAASL
                     DKLHASLASSTTFVEAHDVASRSTVLAERLNRRLVSLADLLLRTRELIGIQWWLMVWV
                     LFSNAILVSGLQEGRSASLENEPQAEREFQLRLVPITIFGWVCFMLFGLVTGEEVAVS
                     NWTWPVFGFIWTSVVMAIITRYMKIAVSPV"
     gene            complement(587..>1454)
                     /locus_tag="PKO06_21950"
     CDS             complement(587..>1454)
                     /locus_tag="PKO06_21950"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HNV72388.1"
                     /translation="AEAISKATALIGPCTSFPLDELTMHVQYHDLQVLNRELAGRKVL
                     DLNADSDKGFIVTRVRRGPTLISITTDTTLEYGDIVRIVSYVGRNEQIERLFGNSVRD
                     LTETDYLSVSLGVVAGLLIGMIPFPIPGNGAAGQGVFRLGLAGGPLLAGLILGWYGRL
                     GPIIWTLPPNASLTLRQLGLHLFLAGVGLKAGGHVMPVLQTHGFDLLAAGTVITLSSA
                     LSAMIIGYRWLKIEPTELLGVVSGIHTQPAVLAFSCDLSGSTVPNSSYAAVQPVCLMS
                     KIILAQVLALWA"
BASE COUNT          341 a          473 c          390 g          250 t
ORIGIN      
        1 ctaccgggct gaccgcgatc ttcatgtaac gggtaatgat cgccatgacc accgaagtcc
       61 agatgaagcc aaagaccggc cacgtccagt tcgacacggc cacctcctca cccgtcacga
      121 ggccgaagag catgaagcag acccagccaa agatggtgat cggaaccaga cgcagctgaa
      181 actcgcgctc tgcctgaggc tcattttcca gactcgctga tcgcccctcc tgtaaccccg
      241 acaccagaat tgcgttcgag aacaacaccc aaaccatcag ccaccactgg atgccgatca
      301 gctctcgcgt gcgcagcagc agatccgcga gcgagaccag ccggcgattc agacgttccg
      361 ccagtaccgt cgagcgactg gcgacatcgt gcgcttcaac aaaggtagtc gacgaagcca
      421 gggaagcgtg cagcttgtcg agcgatgccg cgtgatcagc cgaggctttg atgcgatcga
      481 acggaatttg cccgtcggcc caccagacga tagccaggaa caccaggacc gcgccgttca
      541 gcaccaggaa gcttctcatc ggaccagaca tgtcatgttc ctttcttcac gcccagaggg
      601 ccaaaacctg cgccagaatg atcttcgaca tgagacagac gggttgcacg gcggcgtatg
      661 agctgttcgg cacggtcgac ccgctcagat cacaggaaaa ggccaggact gccggttggg
      721 tgtggatccc cgacaccact ccgagcagct cggtgggctc gatcttcaac catcgatacc
      781 caataatcat ggctgacaga gcactggaaa gcgtgatcac cgtcccggct gccagcagat
      841 cgaacccatg cgtctgcagc accggcatca catgaccgcc ggccttcaac cccacgccgg
      901 ccagaaacag atgcagtccg agttgcctca gagtcagact cgcatttggc gggagtgtcc
      961 agataatcgg tcccaggcgg ccataccatc cgagaatcag tccggccagc agtgggccac
     1021 cagccagccc gagacgaaag accccctgcc ccgcggctcc attccctgga atcggaaagg
     1081 gaatcatacc aatcagcaac cccgcgacca ctcccaacga cacggaaaga tagtcggttt
     1141 cggtcagatc acgcaccgag ttcccgaaca gacgttcgat ctgctcattg cgcccgacgt
     1201 aactgacgat ccgaacgatg tctccgtatt cgagggtcgt atcagtcgtg atcgatatca
     1261 gtgtcggtcc gcgccgcacc cgcgtgacga tgaacccttt gtccgagtcg gcattcaggt
     1321 cgagcacctt tcgaccagcg agttcacggt tcaacacctg caggtcgtga tactgcacat
     1381 gcatcgtcaa ttcatcgagc gggaaactgg tacacggtcc gatcagggcc gtcgctttgc
     1441 tgatcgcctc ggcg
//
LOCUS       DAORHD010001390         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_122198_length_1452_cov_3.815319, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001390 DAORHD010000000
VERSION     DAORHD010001390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122198_length_1452_cov_3.815319"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1452
                     /locus_tag="PKO06_21955"
     CDS             <1..>1452
                     /locus_tag="PKO06_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72389.1"
                     /translation="LELLRSQDPMRFRETISAVIGGASPEVRYHALRLLRSVDMDAAL
                     ECLRECVRDAEPVSRLKAVRELTLIPFEHTEVLLLDVMGCESRTLILVAASICVAMNP
                     SPELPVKLYDMFALARGEKQQILKMLLHLCVNTLKESGLLKVGVQEYLAHLQQQIQVR
                     KHELQKRFMMRDLSDVDPDTRLTAMRWLQDYIEDPEVLSACRERQSVETDPDCRLLLK
                     TMLDSMAPSVAVPGETTVPPSPPTPQQELQEMQQRLKGGGPMGQERNRLRDLFEREEQ
                     KSLQIQVVRLSARYGETEDVPWLRALLGHQNPAVVAEAIRGVGRLDLDTLLHTMNRWL
                     THADPRIRKAGLEVYLQHDKEQALGYIRHMIDSPQPVGRKAAIALLMMLDYATAEPFL
                     VRMYAQEPRSGLRDQVAAILSTNPSMHGLLALFDGTHDSGGELLDEEEGVWATALANA
                     EKVMKRPAVALEQECARRHTQQREAWRAATAKAV"
BASE COUNT          286 a          409 c          459 g          298 t
ORIGIN      
        1 ctcgaactgt tgcgatcaca ggatccgatg cgctttcgcg agaccatttc ggcggtgatc
       61 ggaggggcat cccctgaagt gagataccat gccctgcgcc tgctgcgatc ggtcgacatg
      121 gatgccgcgc tcgaatgtct gcgtgagtgc gtgcgcgatg cggaaccggt gtcgcgattg
      181 aaagccgtgc gtgaactgac cttgattccg ttcgagcata ccgaggttct gctgctcgat
      241 gtcatgggct gcgaaagccg cacgctgatt ctggtggccg cgagtatctg cgtcgccatg
      301 aatccgtctc cggaactgcc ggtgaaactc tacgacatgt ttgcgctggc tcgcggcgag
      361 aaacagcaga ttctcaagat gctgctgcat ctctgcgtga acaccttgaa ggaatccggt
      421 ttgctgaagg tcggagtgca ggagtatctg gcgcatctgc agcaacagat tcaagttcga
      481 aaacacgagt tgcagaaacg tttcatgatg cgagatctgt cggatgttga cccggacact
      541 cggttgacgg caatgcggtg gctgcaggac tatatcgaag atcccgaggt gctttctgcc
      601 tgtcgcgagc gacagagcgt cgaaactgac ccggactgtc gtctgctgct gaagaccatg
      661 cttgactcga tggcgccgtc cgttgccgtt ccgggcgaga cgacggttcc tccctctcct
      721 ccgacccccc aacaggaact gcaggagatg cagcagcggc tgaagggcgg cggtccgatg
      781 ggccaggaac ggaatcgttt acgcgacctg ttcgaacgcg aagaacagaa gtcgctgcag
      841 atccaggttg ttcgtttgtc tgcccggtac ggagagaccg aagatgtacc gtggcttcga
      901 gcgttgcttg gtcaccagaa tcctgccgtg gtggcggaag ccattcgcgg ggtcggtcgt
      961 ctcgatctgg acacgctgct gcacaccatg aaccgctggc tgacacatgc cgacccgcgg
     1021 attcgcaaag ccggtctcga agtctatctg caacatgaca aggagcaggc cctcggctat
     1081 atcaggcaca tgatcgattc gccgcaaccc gtgggtcgaa aagcggcgat cgctctgctg
     1141 atgatgctgg actatgccac ggctgaaccc tttctggtgc gtatgtatgc tcaggagccc
     1201 cgttccggat tgcgtgatca ggtcgccgcg atcctgtcga cgaatccttc catgcacggg
     1261 ttgctggcgc tgttcgacgg aacgcatgac tcggggggtg agcttctgga cgaggaggaa
     1321 ggggtctggg ccaccgccct tgccaatgcc gagaaagtga tgaagcgccc tgcagtggca
     1381 ctcgagcagg agtgcgcccg tcgtcatacg cagcagcgcg aggcctggcg agccgccacc
     1441 gcgaaagccg tt
//
LOCUS       DAORHD010001391         1452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_122236_length_1452_cov_2.959914, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001391 DAORHD010000000
VERSION     DAORHD010001391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122236_length_1452_cov_2.959914"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKO06_21960"
     CDS             <1..350
                     /locus_tag="PKO06_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72390.1"
                     /translation="DILQHRVHLGGYPISGLDYLYKDFFPRTHRLLHYQPRKSMLVDS
                     TYQQVSTEIMISGLFSTWKAYVQRHYTPTEEPLMITYGEDLTGIHLFPGVSDYHRHDL
                     PDLGARELCFAQL"
     gene            365..1036
                     /locus_tag="PKO06_21965"
     CDS             365..1036
                     /locus_tag="PKO06_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72391.1"
                     /translation="MSRKTELLMETFELIRDLSDNLPADRDFGGKSRREWTFRKTRLI
                     YLLILNWMINHGKQAYELFITSRITLANVLVRELVQLSATLFHLLETIEAAVKERRVD
                     RLDADLRRFMIQDQQDRKVPLAQEMLKFIEKVNRRFSGFFTAFNELQLAIAPDYLNAL
                     ATAETEQGQPIDPERFLEGSQAAEVGAKNFHSALIVCAFCRNRMVDLLPMLERLCEPP
                     PGMHS"
     gene            complement(1216..>1452)
                     /locus_tag="PKO06_21970"
     CDS             complement(1216..>1452)
                     /locus_tag="PKO06_21970"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72392.1"
                     /translation="PIQVTARFSQTVVEVSIEDPGPGFEYEQIKLKSGKDLEKMLARG
                     VNKGKGWGLAIMQSVSQGLFWNHRGNRITLLFQK"
BASE COUNT          326 a          441 c          384 g          301 t
ORIGIN      
        1 tcgacattct tcagcaccgg gttcacctcg gcggttaccc gatatccggg ctcgactact
       61 tgtacaaaga ttttttcccg cgcacccaca ggctcctgca ttatcaaccg agaaagagca
      121 tgctcgtcga ctcgacgtac cagcaagtca gcaccgaaat catgatcagc ggcctgtttt
      181 cgacctggaa agcgtacgtg caacggcact acacacccac cgaggaaccg ctgatgatta
      241 cctatggcga ggacctgacc gggatccatc tgtttcccgg ggtatcggat tatcatcgcc
      301 acgacctccc agacctcggc gcccgggagc tgtgttttgc ccagctctag aaagagagga
      361 tcaaatgagc cgaaaaactg agcttctgat ggaaacgttc gagttgatcc gcgatctcag
      421 cgacaacctg cctgccgacc gtgatttcgg cggcaaatcc cgtcgagagt ggacctttcg
      481 caagactcgc ctgatttacc tgttgatcct caactggatg atcaaccacg gaaaacaggc
      541 ctacgagtta ttcatcactt cgagaatcac gctggcgaac gtgctggtac gcgaactggt
      601 acagctttcc gcgaccctgt ttcatctgct cgaaaccatc gaagccgcgg tcaaggagcg
      661 ccgcgtggat cgtctggatg cggacctgcg gcgcttcatg atccaggatc agcaggatcg
      721 gaaagtgccg ctggctcaag agatgctcaa attcatcgaa aaagtgaatc gccgcttttc
      781 gggattcttt accgcgttca atgagttgca gctggcgatc gcccccgatt atctcaacgc
      841 tctggccacc gccgaaacag aacaaggaca accgatcgac ccagagagat ttctggaagg
      901 gagccaggcc gccgaggtgg gcgcaaagaa tttccattca gcgctgatcg tctgcgcctt
      961 ctgtcgtaat cgcatggtcg atctgctccc gatgctcgaa cgactctgcg aaccaccccc
     1021 gggcatgcat tcctgatctc acgtcaacga cgccaccgat acgcgccgga tcccgtgccg
     1081 gcatcaaccc cggcggaatg acgcctcagg cgttcgtggt gtgagcacga tcagcgcccg
     1141 caaaagcgag tcagcctgcc gacatgaaag cgagggagag agaccgggcc gcggtctagg
     1201 cgcggtcgcg ccacactact tctgaaacag cagggtgatg cggttgccgc gatgattcca
     1261 gaacaggccc tgagaaaccg attgcatgat cgctaatccc caacccttgc ctttgttgac
     1321 gcctctcgcc agcattttct cgaggtcttt accgcttttg agcttgatct gttcgtattc
     1381 aaaacccggg cccgggtctt cgatactgac ctcgaccacc gtctgactga accgggcggt
     1441 gacctgaatc gg
//
LOCUS       DAORHD010001392         1449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_122681_length_1449_cov_2.977762, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001392 DAORHD010000000
VERSION     DAORHD010001392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122681_length_1449_cov_2.977762"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKO06_21975"
     CDS             <1..790
                     /locus_tag="PKO06_21975"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72393.1"
                     /translation="GNFSVRMSTTGAHEFVELAKAFNQMARGVAEGKLLSRFVSEAVR
                     TAAGDDARGRAAEKGEFIEVSVLFAGLADFKYLLEHRSPHEVVVLMNRFLEAMSQEIR
                     AHGGDIDKFIGEKILAVFHPTRLGGAPAAATAAVKAAEAMRRRARQLAPDLTKPLGIG
                     IVSGPVLAGIMGTEEVRLEYTVIGDTVNLASRLGDLAMKISPSRDVLKSTEASVGGVV
                     LESRTFTLLQAGPEPRTTERFQQLHLPPIKGKTRSVDAYLVTDA"
     gene            869..>1449
                     /locus_tag="PKO06_21980"
     CDS             869..>1449
                     /locus_tag="PKO06_21980"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72394.1"
                     /translation="MAYRRSIIAGASEDRLNKLIEERLKAAPDTAEIDNRIWDLFGEE
                     WTVMFTDLSGFSRNVAAFGIIHFLQTIYESERILVPIIEQHDGILLKVEGDSMLVIFR
                     NPCKAIHAALSMQSETQRYNRDRRGEDHVLVCIGLGHGRVLRIGDSDVFGQEVNAASK
                     LGEDTAKAGQIMVTDAVRERAKDMSGVKFAPID"
BASE COUNT          307 a          412 c          431 g          299 t
ORIGIN      
        1 gggcaacttc tcggtgcgca tgtcgaccac cggagcccac gagtttgttg aactggcaaa
       61 ggcattcaac cagatggccc gtggagtggc tgaaggaaag ctgcttagtc gctttgtttc
      121 tgaggctgtg cgcaccgccg ccggagacga tgcgcgtgga cgggcggctg aaaaaggcga
      181 attcatcgaa gtttcggtgt tgtttgcggg gctcgctgac ttcaagtatc tgctcgagca
      241 tcggtctcct cacgaggtgg ttgttctcat gaatcgcttt ctcgaagcga tgtcacagga
      301 gattcgcgcc cacggcggag atatcgacaa gttcatcggc gagaagattc tggcggtctt
      361 ccatcccaca cgactgggcg gcgcaccggc agcggcaacg gcggccgtga aagcggcgga
      421 agccatgcgg cggcgggccc gacagctggc cccggatctg accaaacctc tgggcatcgg
      481 catcgtcagc ggtcccgtgc tggcagggat catgggcacc gaggaagtcc gcctcgaata
      541 cacggtcatc ggtgacacgg tgaacctcgc ttcgcgcctg ggagatctgg cgatgaagat
      601 ctcaccctcc cgtgacgtgt tgaaaagtac tgaagcttca gtcggcggcg tcgtactgga
      661 gtctcgtacc ttcaccctcc tgcaggctgg cccggagcct cgaaccaccg agcgttttca
      721 gcaactgcac ctgcctccca tcaaagggaa aacacgttct gtcgacgcct atcttgttac
      781 tgatgcgtga ttttcggttg ctgaatcggt actgaaacgc tatgctgtta tcgattcgtt
      841 attgaacacg gacgggagga actgcttcat ggcctatcga cgatcaatca tcgccggcgc
      901 ttccgaggat cgcctcaaca agctcatcga ggaacgcttg aaagccgctc cagacaccgc
      961 tgagatcgac aaccggatct gggacctgtt cggcgaggaa tggaccgtca tgttcacgga
     1021 tctttcgggg ttttcgcgaa acgtcgccgc attcggaatc attcactttc tgcagacgat
     1081 ctacgaatca gaacgcatcc tggttccgat catcgagcag cacgacggca ttcttctcaa
     1141 ggtcgagggc gacagcatgc tggtgatctt ccgcaatccg tgcaaggcta tccatgccgc
     1201 tctcagcatg cagagtgaga cccagcgcta taaccgtgac cgacgaggcg aagatcacgt
     1261 actcgtgtgc atcggtctcg gacacggccg ggtgctgcgc atcggtgatt cggacgtctt
     1321 cggtcaggaa gtcaacgccg ccagcaaact cggagaagac acggccaagg ccgggcaaat
     1381 catggtaacc gatgccgtgc gcgaacgggc gaaagatatg tctggcgtga aattcgcgcc
     1441 catcgatta
//
LOCUS       DAORHD010001393         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_122868_length_1448_cov_2.449390, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001393 DAORHD010000000
VERSION     DAORHD010001393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122868_length_1448_cov_2.449390"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..711)
                     /locus_tag="PKO06_21985"
     CDS             complement(154..711)
                     /locus_tag="PKO06_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72395.1"
                     /translation="MTRGNVSLGLVVACLVLAVGVVVIPNLETTVHSRPAEQCRVSRM
                     LFAQAIELAMIEASATQPLEVTLTNFHDIVQRLEKTVGKTASCSVCPVTQQPLLTGSD
                     PKRPSQVFVCCSIHKDDRPEYADVVLPAFPPIDQETLRLREAEFHQKVLQSRHPFGQP
                     QSLWLCTVVLAACAGFIAHRLSGKQ"
     gene            1148..>1448
                     /locus_tag="PKO06_21990"
     CDS             1148..>1448
                     /locus_tag="PKO06_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72396.1"
                     /translation="MKNYRWGHPMKRQNLELKVLFFAILFVVGAVGFQGCKENSTNIP
                     LNSIGSVTEIDGVSISTMGKLSGLVVQDSGTTGIPNISVRLLIASGSIPVETTLTT"
BASE COUNT          370 a          398 c          355 g          325 t
ORIGIN      
        1 ggcccagctc tcccagaaac tcaaaggccg ctgacttcga ccggccaagc caggttctgt
       61 tcaagcgctc aacggtacag aatggctcag gcatcgccag aaagcgaccc attggtcgtt
      121 gccagatgtc aggcgcacgg ttgcgggttt cgcttactgt ttcccggaaa ggcgatgtgc
      181 gatgaaaccg gcgcaggcag ccagaacaac ggtacagagc cacagggact gcggctgtcc
      241 aaacggatgt cggctctgca gcactttctg atggaattcc gcttctcgca gtctgagggt
      301 ttcctgatcg atcggcggaa acgcgggaag aacgacgtcg gcatactcag gccggtcatc
      361 tttatgaatg ctgcaacaga caaacacctg actcggacgt ttcggatctg agccagtgag
      421 aagcggctgc tgcgtcaccg ggcagacgga acaggatgcc gtcttgccaa ccgttttttc
      481 gaggcgctga acgatgtcgt gaaagttcgt caacgtgacc tcgagcggct gggttgccga
      541 agcctcgatc atcgccagtt cgatcgcctg cgcgaacaac atgcgtgaga cccgacactg
      601 ctccgcgggc cggctgtgca ccgtagtctc cagattcgga atcaccacca ctcccacagc
      661 caggaccaga caggccacca ctagccccaa agaaacgttg cctcttgtca cacatttctc
      721 tccttgcgtc gttccctgaa caaaaacttt gcatgctgca gggtatcgcg tcacattcta
      781 taacagcgat actactccca acgatacttt ttcgcatccg gaacaacgca cagtatgccc
      841 catgaaaaag gcctccggaa gttattcttc tccagaggcc tctcggacaa tcgcgctggt
      901 tctaccgatg catctctcta cgacgagaca tatgatccta tattaggatt tttatcgctg
      961 tctccctctt ttccagaatt atgttgtcca cgaaaaagtt gtgactcatc gtcaaaagaa
     1021 gtttacagaa tggaccttgg gcaacatgca atgaacctaa cagtttaccc tccgtcgggc
     1081 acttctgcaa aagtgaactc agcaaaaaat cggatcatac atcgtctaaa ccatgccgat
     1141 agtagacgtg aaaaactatc gctgggggca cccaatgaaa cgacagaacc tggaactgaa
     1201 agtcctattc ttcgccatac tctttgttgt tggagcagtc ggatttcagg ggtgcaaaga
     1261 gaatagtacg aacattccgt taaactccat tggttcggta acggaaatcg atggcgtatc
     1321 aatatcaacg atggggaagt tatcgggcct cgttgtccag gattcaggca ctactggaat
     1381 tccaaacatc tcagtgagat tgctgatagc ctctggcagc attcctgtcg aaacaacatt
     1441 aacaacag
//
LOCUS       DAORHD010001394         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123015_length_1447_cov_2.459052, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001394 DAORHD010000000
VERSION     DAORHD010001394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123015_length_1447_cov_2.459052"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(455..>1447)
                     /locus_tag="PKO06_21995"
     CDS             complement(455..>1447)
                     /locus_tag="PKO06_21995"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HNV72397.1"
                     /translation="HLLVLLSGGTSALVAAPPSTGDSVSRLSRHGRGSSRAKFTESVK
                     EELHRVLVKAGLSIVDTNLIRRHCSGIKGGAALRAAMNAGKNVSVLTISDVYGDVPAD
                     IGSGPFAPDPTSFAAALQKAERVSGIPAPVMDHLRAAVMGQIPENLRPEELSPDRWRF
                     EILARPGDALTIACQALEELEETVVPCPMFIDQTWFWGDFPFVRWVKRENLPIGTWVV
                     AIGEAEVAIPATVRPGKGGRASHLVVSLACQFLQTLRPFELAVVATDGVDGTSDLGGG
                     FLQTSDIEPFTIDHLQGTLTAFDSANFLQNIGRSFPGARSGTNFGDILLFRWLG"
BASE COUNT          350 a          431 c          382 g          284 t
ORIGIN      
        1 tgctcaaaga gtcgagcaga tcatcgggct gatcctccat ccaaccgaca aaacgatcat
       61 ccatcatcag ctccgtgatt ccgaccatcc ctgggtgcac cattgtccga ctccttgtta
      121 cttgctactc gctacgaggt ttccttcgtg acatgttttt catcggcaaa agtgcaggga
      181 atttacagac tcaatttttt cgaaacgggt gcacacgttt attccgggct ctgaaagcgg
      241 gtttcgaagc catctttttt tgttcagaac aacaagcgaa caagcgtgca gagtgtaggg
      301 agtcgagtag cgcgtgcaaa agagaaccac ctgaacgacc gacggaaaaa ctgccggtga
      361 tgctgccgaa gtgacaggca cccgccagaa gcgaattcag aaacgggtca gttcaaacac
      421 cgacactcgt cggtggttca agaatcagtg ggcttcatcc cagccagcga aacagcagaa
      481 tgtccccaaa gttcgtgcca cttcgtgccc ccggaaaact gcgaccgatg ttctgcagga
      541 aattggcaga atcaaaggct gtcagtgttc cctgcaggtg atcgatcgta aagggctcga
      601 tatctgacgt ttgcagaaaa ccacccccga gatccgaggt tccatccact ccatctgtag
      661 caacgaccgc cagttcgaac gggcgcaagg tctgaaggaa ctgacatgcc agcgagacga
      721 ccaggtgcga ggcgcgcccc cctttcccgg gtcgcaccgt ggctggaatg gcaacttccg
      781 cttcacctat ggcgacaacc caggtgccga tcggcagatt ttcacgcttc acccagcgca
      841 caaacggaaa atcgccccag aaccaggtct ggtcgatgaa catcggacat gggacaaccg
      901 tttcctcgag ttcttcgaga gcctgacagg caatcgtgag cgcatctccc ggccgtgcca
      961 gaatttcgaa ccgccagcga tccggtgaca gctcttccgg gcgcaggttt tcaggaatct
     1021 gacccatgac ggcggcgcga agatgatcca tgaccggtgc cggtattccg gaaacgcgct
     1081 cggccttctg taacgcagcg gcaaaagatg tcgggtccgg ggcgaatggc ccggaaccaa
     1141 tatctgcggg aacatcaccg tacacgtcgg aaatcgtgag cacactgacg ttctttcctg
     1201 cattcatagc tgctctcaag gctgccccac ccttgatccc cgagcagtga cgacgaatga
     1261 ggttggtatc cacaatcgac aagccggctt tcaccagcac ccgatgcagt tcttctttca
     1321 cagactcggt gaacttcgct cgcgacgagc cccgcccatg gcgagaaagt ctcgagaccg
     1381 agtctcccgt cgacggcgga gctgccacca gagcagacgt tccgcccgaa agcagcacca
     1441 gaaggtg
//
LOCUS       DAORHD010001395         1446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123119_length_1446_cov_2.971963, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001395 DAORHD010000000
VERSION     DAORHD010001395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123119_length_1446_cov_2.971963"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PKO06_22000"
     CDS             complement(<1..800)
                     /locus_tag="PKO06_22000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72398.1"
                     /translation="MAKKRHTTSSPSSDPVPAPVTSSRSRTPLQDRVYERIQQLRADN
                     SSEYAFRLIERLLCRQSPEPEWFYLKGCCSMDLGWFALGAESFAAASFFCPEQVDYRL
                     ALANSLLLTGQEAKGLALIRDVLKKDAEHFQAHLLLGQHHEQREEWRSALSHYDRIKS
                     QTDQPGYPQYLCHLALCQMHMGLERQALEHLEEAWRLDGDDPEILFAKGVANGQIGEY
                     ETALIDMEEVIKRQPADPEALAYSAMFLYMLGQKPQAVARLEKAYELAP"
     gene            934..>1446
                     /locus_tag="PKO06_22005"
     CDS             934..>1446
                     /locus_tag="PKO06_22005"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV72399.1"
                     /translation="MADEAKKTVFRRVVVTEEQKKKYLVWNTPPYLGKPVELGKEKFT
                     IGREEGRDLVIPSEMVSRMHAEIFEKDGAYYIRDTNSSNGSFLNNAQLEPEQAVQLKH
                     TDILKFDQYEFIFVDSARADLWETLKPLNRAGAITVAFYSPKGGTGLTSVVVNLANAL
                     SEGGKRVAIAD"
BASE COUNT          332 a          374 c          439 g          301 t
ORIGIN      
        1 ggagccagtt cataggcctt ctcgagccgc gcgaccgcct gaggtttctg accgagcata
       61 tagagaaaca tggctgagta agcgagggct tcagggtcag ccggctggcg tttgatgacc
      121 tcttccatgt cgatcagtgc cgtttcatac tcgccgatct gaccgttggc gacacctttg
      181 gcaaagagga tttccgggtc atcaccgtcg aggcgccacg cttcttcgag atgttccagg
      241 gcctgacgtt caagccccat atgcatctga cagagcgcca gatggcacag atactgcgga
      301 tacccgggtt gatcggtctg tgatttgatg cgatcgtagt gcgacagcgc tgaacgccac
      361 tcttcccgtt gctcgtggtg ttgtccgagg agcaggtgtg cctgaaagtg ttcggcgtct
      421 ttcttgagca cgtcgcggat cagggcgagc cctttggcct cctggccggt gagcagcagc
      481 gagttggcga gagccagacg gtaatcgacc tgttccgggc agaagaagct ggctgcggcg
      541 aatgactcgg caccaagcgc aaaccagccg agatccatgg aacagcagcc tttgagatag
      601 aaccattcgg gctctgggct ctgacgacag agcaagcgct cgatcaaacg aaacgcatac
      661 tcggaactgt tgtcggcacg cagctgctga atccgctcgt aaacgcgatc ctgcagggga
      721 gtgcgcgagc gactcgaggt gaccggcgcg ggaactgggt cggaagatgg agatgaagtt
      781 gtgtgacgtt tttttgccat acgggaaccc gtccaaggat acctgaggtg accgctgtaa
      841 agcaagacct gcattcatca gggttatcag aaagttgaac cgctgggagg ggtgtgctat
      901 actcgcatga ctttgtctga gggagagtgg atcatggctg atgaagcaaa gaaaacagta
      961 tttcggcgag tggtggtcac ggaagaacag aagaagaagt atctcgtctg gaatacccct
     1021 ccgtatctcg gcaagccggt agagctcggc aaagagaaat tcaccattgg tcgtgaagag
     1081 ggtcgcgatc tggtgattcc cagcgaaatg gtgtcgcgca tgcacgccga gatcttcgag
     1141 aaagacggcg cctactacat cagagacacc aactcatcga acgggtcgtt tctgaacaat
     1201 gcgcagctcg aaccagaaca ggccgttcag ctgaaacata ccgacattct gaaattcgat
     1261 cagtacgagt tcattttcgt cgattccgct cgggcagatc tctgggagac cctgaaacct
     1321 ctgaatcgtg ccggagccat caccgtggcc ttctacagcc cgaaaggcgg caccggtctg
     1381 accagtgtgg tcgtgaacct cgcgaatgct ctttccgaag gtggcaagcg agtggcgatt
     1441 gcggac
//
LOCUS       DAORHD010001396         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123226_length_1445_cov_3.238129, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001396 DAORHD010000000
VERSION     DAORHD010001396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123226_length_1445_cov_3.238129"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="PKO06_22010"
     CDS             complement(<1..112)
                     /locus_tag="PKO06_22010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV72400.1"
                     /translation="MKKFLVTGGAGFIGSNLVDALLEKGFEVRVLDDFSSG"
     gene            409..>1445
                     /locus_tag="PKO06_22015"
     CDS             409..>1445
                     /locus_tag="PKO06_22015"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HNV72401.1"
                     /translation="MRRNPQYRHTGYRQLEERYVSETLRRNPVAATWMGEHAYDDLLP
                     DTGAEAVEREIAGMKEMRTLWEHLPERELSLDERLDRRLVLYQLQNDLFLQEDARRWR
                     LGSDLARIIGDSLFPLFVRDFAPWHQRVERMVSRLKAAPAFLMAGRSLFQAVPPLFMK
                     VYLESAQRLPDLVDAIAGSIAGRVPEFLIHEFRKASLEAKKALHGHVHWLEQAIAPKA
                     AGDWALGPAAFQGLLTVRQLGLTPGDLRELGESTLQKCRSRMQALAKKMVGEPNRDAA
                     LRRIQQNAPKSFAQALDVYRDATARCRAFIDLNGFATLPGEETLEIMETPSYMAHLIP
                     FAAYISPERSA"
BASE COUNT          287 a          436 c          417 g          305 t
ORIGIN      
        1 gaccgctcga gaaatcatcg agcactcgga cttcgaaccc cttttcgagc agagcatcga
       61 cgagattgga gccgatgaaa cctgctccgc cggtcaccag aaatttcttc atgtcacgga
      121 cctctcttcc ttgtcgggtg tcgtgcgttg tcatcatact gccacatcgc ctgtccggca
      181 tcaagcgacc accgcacgat gcactccctg ccgcgacggc gaatcttaga gcgatgccac
      241 ggggcaaacc caacccggag aatcacatga ctctgcacat gactgccaga aactcacggg
      301 tctcttgttc gtggaacagg ttcgcgcaag cgtcgaatgc cgggcggcca cagggtcggc
      361 tggccgtggt atactgcggc aacaccacca tgtcgggagg gactgattat gcgacggaat
      421 ccacagtatc gtcatacggg atatcggcaa ctcgaagaac gctatgtgtc agaaacgctg
      481 cgccggaacc cggtcgccgc cacctggatg ggtgagcatg cctatgatga tcttctgcct
      541 gacaccggag ctgaagccgt tgagcgcgag attgccggca tgaaggaaat gcgcacgctg
      601 tgggagcatc tgcccgaacg cgagctcagt cttgacgaac gtctggatcg tcgcctggtt
      661 ctctaccagc tgcagaatga cctgttcctg caggaagacg cgcgccgctg gcgcctgggt
      721 agcgatctgg cccgcatcat cggtgattca ctttttccac tctttgtgcg tgattttgct
      781 ccatggcacc agcgcgtcga acgcatggtc tcgagactca aggccgcgcc ggcttttctc
      841 atggccggtc gctcattatt ccaggcggtg ccgccgcttt tcatgaaggt ctatctggaa
      901 tccgcccagc ggctgcctga tctagtcgat gccattgccg gttcgatcgc gggtcgggtt
      961 cccgagtttc tcattcacga gttccgcaaa gcctcacttg aggcgaagaa agcattgcat
     1021 ggacatgtac attggctgga acaggcgata gctcccaagg cggctggtga ctgggctctt
     1081 ggcccggcag cttttcaagg acttctgacg gtgagacagc tcggactgac tcccggcgat
     1141 ctgcgtgaac tcggtgagtc aaccttgcag aagtgccgct cacgcatgca ggcactcgcg
     1201 aagaagatgg tcggcgaacc gaatcgcgat gccgctctcc gccgcattca gcagaatgcg
     1261 ccgaaatcct ttgcccaggc gctcgatgtg tatcgtgacg cgaccgcccg gtgccgggca
     1321 ttcatcgacc tcaacggttt tgccacgttg ccgggggaag agacgctcga gatcatggag
     1381 acgccttctt acatggcgca tctcattccc tttgcggcct acatctcgcc cgagcggtcg
     1441 gcaag
//
LOCUS       DAORHD010001397         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123229_length_1445_cov_3.179137, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001397 DAORHD010000000
VERSION     DAORHD010001397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123229_length_1445_cov_3.179137"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PKO06_22020"
     CDS             <1..945
                     /locus_tag="PKO06_22020"
                     /inference="COORDINATES: protein motif:HMM:NF012366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72402.1"
                     /translation="SVGGNSFGVEFDSWHQNAEDLNSSHIAVIKNSTLIPHYRRVDTT
                     VAPLSTHFRTASIFYIRVEYIGPSRNLRVFANVGAAIPAGATPIINHIFSSSLTAMLG
                     GLTLANFGFTASTGAAWEAHNLRSWKLETGAPPATVQALSNKFKVYYPHAYDPASSKY
                     GETLDAFPLPMGLCDGAAVNYYSRKDKKFYLYYIGGGTGEGNTVDPLLYKYDFDNPAV
                     WSPLDPGDDNWTQKTGVAACAWGDEIFIFGGASGTVKDTGIAYNPDTNRYRTLTNLPA
                     TRTYQSAVPVGPYIYLLGGATAVSGTGNGITTIYRYKP"
     gene            963..>1445
                     /locus_tag="PKO06_22025"
     CDS             963..>1445
                     /locus_tag="PKO06_22025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72403.1"
                     /translation="MKFSARQQNILLSCRCLTGRWPGGASRSGYTLMEMITVILVVAA
                     VASVAMPAADGFRSQQQVNAQASVLVGDIRYARSWAMSEQGFCRLVFSNDKASWVVQE
                     HCVAGDPVLGEPTTTWAGEPATTYTANPIYWRSIIEEPAREVEGAIDVQLTPDVNPTA
                     I"
BASE COUNT          323 a          384 c          419 g          319 t
ORIGIN      
        1 tcagtagggg ggaactcttt tggagtggag ttcgactcgt ggcaccagaa cgccgaggac
       61 ttgaacagca gccacattgc tgtgatcaag aactccaccc tcatcccaca ttatcgacgg
      121 gtcgatacca ccgttgcgcc tctttcaaca cacttcagaa ctgcctccat cttttacatt
      181 cgagttgaat atattggccc atcaagaaat ctcagagtct ttgccaatgt cggtgccgcg
      241 attccggcag gggcaacccc catcatcaat cacatctttt ccagttcttt gacggccatg
      301 cttggtggct tgactctcgc caatttcggg ttcactgctt cgaccggagc cgcatgggaa
      361 gcccacaatc tccgaagctg gaagctggag accggagccc ctcccgcgac tgtgcaggca
      421 ctttccaaca aattcaaagt gtactacccg catgcctacg acccggcgtc aagcaagtat
      481 ggcgagacgc ttgatgcatt tcctttaccg atggggttgt gtgacggggc ggcagtgaat
      541 tactacagtc gaaaagataa gaaattctat ttgtactaca tcggtggggg cactggagag
      601 gggaataccg tcgatcccct cttgtacaaa tacgatttcg acaacccggc ggtgtggtct
      661 ccacttgacc cgggagacga taactggacg cagaaaacgg gtgtggcggc atgcgcctgg
      721 ggggatgaga ttttcatctt cggcggcgcg agtggtacgg tgaaagatac gggtattgcg
      781 tacaacccgg acaccaatcg ataccgcact ctgacgaacc ttccggcgac gcgaacctat
      841 cagtcggcgg tgccggtcgg gccatacatc tatctgctgg gtggagcaac cgccgtatcc
      901 ggtaccggaa acggcatcac gaccatctac cggtacaagc catgaagatt gagaaaaggg
      961 gcgtgaaatt ctcggcaaga cagcagaata ttctgttgtc ttgtcggtgc ctgacgggac
     1021 ggtggcctgg tggagcctcg cgcagcgggt acacgctgat ggaaatgatc acggtgatcc
     1081 tggtggtcgc agcggttgct tcggtcgcca tgccagcggc tgatggtttc aggtcacagc
     1141 agcaggtgaa tgctcaggct tccgtactag ttggcgacat tcgctatgcg cgaagttggg
     1201 ccatgtccga gcagggattc tgtcgcctgg tgttcagtaa tgacaaggct tcatgggtgg
     1261 tccaggagca ttgcgtcgcg ggcgatcctg ttctcgggga accgaccacg acctgggctg
     1321 gagaaccggc taccacgtat actgcgaacc cgatttattg gcggagtatc attgaagaac
     1381 cggctcgtga agttgaggga gcaattgacg tacagttgac tccggacgtg aaccccacgg
     1441 cgatc
//
LOCUS       DAORHD010001398         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123349_length_1444_cov_4.246940, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001398 DAORHD010000000
VERSION     DAORHD010001398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123349_length_1444_cov_4.246940"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKO06_22030"
     CDS             complement(<1..410)
                     /locus_tag="PKO06_22030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV72404.1"
                     /translation="MANPLTVANRTWTYADLLKLPEEQRCEIIDGEYFDMTPAPNRLH
                     QEILGNLFRQIINQLDGKPCKVYFAPFDVRLPDSDDQPDGEIRNVVQPDLVVICDEKK
                     LDDRGCRGAPDLAVEILSPSTAGKDQVKKRRLYER"
     gene            complement(434..1066)
                     /locus_tag="PKO06_22035"
     CDS             complement(434..1066)
                     /locus_tag="PKO06_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017089868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72405.1"
                     /translation="MNQRIKILSGLPFVFVSKSVHLFKREKSMMPGNAPERRGFTLME
                     LLLVIAVLAVVAAVGAPQFFRSSVALADDARLNGLKSNYALVMQAVKARLEHERANPA
                     VASPLKETGGNGDYADSGSNIRLLVDNGFLPKTATTWETRLGKSGNFRVIGVSPSNKL
                     PPFLQPFSRFQVEAVNPDAVAVNITDELETKTAAGTPVPDAWAEIWSALH"
     gene            complement(1063..>1444)
                     /locus_tag="PKO06_22040"
     CDS             complement(1063..>1444)
                     /locus_tag="PKO06_22040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72406.1"
                     /translation="GGMWLLFLQQGAYTMTTQRNDAVTKLTSTYQLMFKEIHETSRQA
                     IGDFTPESTEPLPHDRVATGAALPYLFQYDSTNAEILCKGNPVLNDVLATFTWDDLLK
                     VVKVELQNRTTGDRLNFLVAPRNP"
BASE COUNT          286 a          414 c          441 g          303 t
ORIGIN      
        1 cgttcataga ggcgacgttt cttgacctga tctttgccgg cagtcgacgg agagagaatt
       61 tcaactgcca ggtcaggggc accacgacaa ccgcgatcgt ccagtttttt ctcgtcacag
      121 atgaccacca gatcaggctg aacgacatta cgaatctcgc cgtcgggctg atcgtctgag
      181 tccggcaacc tcacgtcaaa cggggcgaaa tacactttgc agggcttgcc atcgagttgg
      241 ttgatgatct gtcgaaacag gttgccgaga atttcctgat gcaggcggtt gggggccggc
      301 gtcatgtcga agtattcgcc atcgatgatc tcgcagcgct gttcttcagg aagcttcagc
      361 agatcggcat aggtccaggt tcggttcgcg accgtcaacg ggttcgccat agtttgtctc
      421 ccttctctgt cggtcagtgg agggcggacc agatctcggc ccaggcatcg ggcaccggcg
      481 ttccggcggc agtcttggtc tcaagctcgt cagtgatatt caccgcgacg gcatcggggt
      541 tgaccgcttc gacctgaaac cgggaaaacg gttgcaggaa cggcgggagc ttattcgagg
      601 gcgaaacacc gatcacgcga aagttgccgg atttgccaag gcgagtttcc caggtcgtcg
      661 ccgtcttggg caggaaaccg ttatcgacca gcaggcgaat gttcgagccg gaatccgcgt
      721 agtcaccgtt cccgcccgtt tctttcagtg gcgaagcgac ggcagggttc gcccgctcgt
      781 gttcgagacg agccttgacc gcctgcatga ccagcgcgta gttggacttg aggccgttga
      841 gccgcgcatc gtcggccaga gcgaccgacg agcgaaagaa ctgcggggcg ccgaccgcgg
      901 ccaccaccgc cagcacggca atgaccagca gcagctccat cagcgtaaac ccacggcgct
      961 caggtgcgtt tcctggcatc atgctctttt ctcgtttgaa caggtgtaca gacttcgaca
     1021 cgaagacgaa gggcaaaccg ctcagaatct tgattctctg gttcatgggt ttcttggggc
     1081 gacgaggaag ttgaggcggt cgccggtggt gcggttctgc agctcgacct tgacgacctt
     1141 gaggagatcg tcccaggtga aggtggccag aacatcgttc aacacggggt tccccttaca
     1201 caagatctcc gcgttggtgg aatcatactg aaagagatac ggcagcgccg cgccggtcgc
     1261 cacccgatcg tgcggcaacg gctcggtcga ctcaggcgtg aaatccccga tcgcctgccg
     1321 actggtttcg tgaatctcct tgaacatcag ctggtaggtg ctcgtgagtt tcgtgacggc
     1381 atcgttgcgc tgcgtggtca tggtgtacgc gccctgctgc agaaagagca gccacatccc
     1441 gccc
//
LOCUS       DAORHD010001399         1444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123370_length_1444_cov_3.357091, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001399 DAORHD010000000
VERSION     DAORHD010001399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123370_length_1444_cov_3.357091"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PKO06_22045"
     CDS             complement(<1..1144)
                     /locus_tag="PKO06_22045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72407.1"
                     /translation="MENLFNSLNSLEPHVIIGVSLPLVFVAIWFVRRRRVQTLATVED
                     HWAKTQATILEHSKRPIPEGETRNQLVLTLRSLQSQPERVLVDAGYAAAATGLLLRLN
                     QPVEAEKLLKQAQQKYPGSPAWQDLEIDLLVAKRAADAKSVDRLCDACKRRPDQVEWH
                     KRLVAWHVAGGQPNPKTLDVLQAWGINTKDTRALQIVCEHHDRAKAYTKRLLPLFEAA
                     CQVERDKVRWLYALARCRHHAGDQEGAREALSQVLRLEPRHQAALEFQSLLIGPPPTA
                     TVSTISPSPAPTTTATVVSAGEPFTLPERYTDVVEIGRGGMGTVYRAFDQILSRPVAI
                     KSLHESLAQQEGLRERFLAESRTLAALDHPCIPKVFDVSVNAPSFIA"
     gene            complement(1202..>1444)
                     /locus_tag="PKO06_22050"
     CDS             complement(1202..>1444)
                     /locus_tag="PKO06_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72408.1"
                     /translation="GRHGPKRDPQKALAAFAQASRFEGAPGYIIRLVAVSLAALERRE
                     EAIQILEQMLKSPTRDPGQKEQDAVMLNRIRSGEKF"
BASE COUNT          275 a          422 c          428 g          319 t
ORIGIN      
        1 acgcgatgaa ggaaggggca ttcaccgaaa catcaaaaac cttgggaata cagggatgat
       61 caagtgccgc cagagtccgt gattcggcga gaaaccgctc ccgcaatcct tcctgctgcg
      121 ccagactctc gtggagcgac ttgatggcca cgggacgcga aaggatctgg tcgaatgcgc
      181 gatagaccgt gcccatgccg cctctgccga tttccaccac atcggtgtag cgctcgggca
      241 gggtgaaggg ttcgccggca gagaccaccg ttgcggttgt tgttggggca ggggatggcg
      301 aaatcgtgga cacggtcgcg gtcggtgggg gcccgatcag cagactctga aactcgagag
      361 cagcctgatg acgtggttcc agacgaagaa cctgactcaa cgcctcccgc gctccttcct
      421 gatcacccgc gtgatgacga cagcgcgcca gagcatagag ccaccgcact ttgtcgcgct
      481 cgacctggca tgccgcttca aagagaggca gcaggcgttt cgtgtaggct ttcgcccgat
      541 cgtggtgttc gcagacgatt tgcagcgctc gggtgtcctt ggtgttgatt ccccaggctt
      601 gcagcacgtc aagcgtcttg ggattgggct gtcctccggc cacgtgccac gccacaagtc
      661 gcttatgcca ctcgacctga tccggtcgac gcttgcaggc atcacataat cgatcgacgg
      721 attttgcgtc tgccgctcgt ttggcgacca gaagatcgat ctccaggtct tgccatgcag
      781 ggcttcccgg gtatttctgc tgcgcctgtt tcagaagttt ttctgcctcg acaggttggt
      841 tcaatctgag gagaaggccg gttgctgccg cggcataccc cgcgtcgacc agcactcgtt
      901 ccggctgcga ctgtagactg cgtaatgtca gtacaagttg gtttctcgtt tcgccctcag
      961 gtatcggtcg cttggagtgc tcgagaatgg tggcctgggt tttggcccag tggtcttcga
     1021 cggtcgccaa ggtttgaact cgacgtcgcc ggacaaacca gatcgccacg aacaccagag
     1081 gcagagaaac gccaatgatg acgtgcggtt cgaggctatt caggctgttg aacagattct
     1141 ccacgagtgc tgcttttctg tgaagctcca gactcgtcag attatatcat gttcctgccc
     1201 gttagaactt ttctccggaa cgtatccggt tgagcatcac cgcgtcctgc tccttctgcc
     1261 cggggtcgcg cgtgggactt ttcagcatct gctctagaat ctgaatggcc tcttcacgac
     1321 gttccagcgc ggcgagactg acggcgacca ggcggatgat atatcccggc gctccctcga
     1381 agcgactcgc ctgggcaaaa gcagccagcg ctttctgggg atcacgcttc ggaccatgcc
     1441 gacc
//
LOCUS       DAORHD010001400         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123532_length_1443_cov_3.262248, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001400 DAORHD010000000
VERSION     DAORHD010001400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123532_length_1443_cov_3.262248"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKO06_22055"
     CDS             <1..409
                     /locus_tag="PKO06_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72409.1"
                     /translation="NTCLLSLQQRLQPSGPEQQETWMKRWWFFRLDLHGDQATVYTMR
                     HPNETERNLDIFGTGFFGSMTYTPNPLEAVWAFHKHPNDILAFLKTPKAEPLWRQWLT
                     FTRAFLWSDLKPAEPNRYLPNQLPETSENPAFR"
     gene            640..1221
                     /locus_tag="PKO06_22060"
     CDS             640..1221
                     /locus_tag="PKO06_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72410.1"
                     /translation="MFRSFSLVCIVLFLSLICVVPAAALDRTISRVEVTQVANPVSFE
                     NSTSYEILYFATVEGIEVEVFPTRVEKEQDFDLILASIVRHYIENEYEANGKFLDETN
                     VEKSTPQEICHLVSKNFVSAAWSKNAQTELIRFLQQNSNSLSLYRLDAYLDYGTDKGD
                     YVSGLDTNPVLFRFGTGVNRDRVTFIQVNQTTD"
BASE COUNT          335 a          466 c          323 g          319 t
ORIGIN      
        1 caacacctgc ctgctcagcc tgcaacaacg actgcagccg tccgggccag aacagcagga
       61 aacctggatg aaacgctggt ggttctttcg cctcgacctg cacggcgacc aggcaacggt
      121 ctacaccatg cggcacccaa acgaaaccga gcgaaacctt gacattttcg gcaccggctt
      181 tttcggcagc atgacttaca ctccgaatcc cctggaagca gtctgggcct ttcacaaaca
      241 cccaaacgac atcctcgctt ttctcaagac ccccaaagcc gaaccactct ggcgccaatg
      301 gctgaccttc acccgcgcat tcctctggtc agatctgaag cctgccgaac ccaaccgcta
      361 cctcccgaac cagctccccg aaacttcgga aaacccggcc ttcaggtaaa tcccgaacaa
      421 acactgcgca aaaataacgt cggcggagat cgtcgagaga tcagttttta cacaccccaa
      481 accgacgtcc atgctcactt ttcacgcacc ccttgtgtgg atgcggtcct ctgccgataa
      541 gaaacctggt acacaatttg ctttattcgg cacctttccg gagtcaactc gttagagcaa
      601 accgtccgga tttctctcag aggtattgtg aggttcgtta tgttccgttc tttttctctg
      661 gtttgtatcg tattgtttct ttctctcatc tgcgtggttc ccgccgccgc tctcgaccga
      721 acgatcagcc gggtcgaagt gactcaggtg gccaaccccg tctctttcga gaatagtacg
      781 agttatgaaa ttctctactt cgcaaccgtc gaaggaattg aagtcgaagt attcccgacc
      841 cgcgtcgaaa aagagcagga tttcgacctg atcctggcct cgatcgttcg tcactacatc
      901 gaaaacgagt atgaagccaa cggcaagttc ctcgacgaaa ccaatgtcga gaaatccacc
      961 ccgcaggaaa tctgccatct ggtcagcaag aacttcgtct ccgccgcctg gagcaagaac
     1021 gcccagaccg aactgattcg cttcctgcag cagaacagta attccctgag cctgtatcgt
     1081 ctcgacgcgt atctcgacta cggcaccgat aaaggtgact acgtcagcgg tctcgacacg
     1141 aatcccgtgt tgttccgctt cggcaccggg gtcaatcgcg accgcgtgac gttcattcag
     1201 gtgaaccaga ccaccgactg atcgtcggct cttcgcacaa cagacggcgc tgctcgaatc
     1261 gggcagcgcc gttctctttt ctcccgtcta cgacctcaat ctcactcgtg cacatgccac
     1321 gacacttgct tcaacagtgt cttttctctg tcggcaaccc ggacatcacc acgttcgtct
     1381 ctcacccgac aaccgagaaa aagggccgca gacgaagttc gtcttcgtct gcggcccatc
     1441 atg
//
LOCUS       DAORHD010001401         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123558_length_1443_cov_2.770173, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001401 DAORHD010000000
VERSION     DAORHD010001401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123558_length_1443_cov_2.770173"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..>1443
                     /locus_tag="PKO06_22065"
     CDS             196..>1443
                     /locus_tag="PKO06_22065"
                     /inference="COORDINATES: protein motif:HMM:NF013604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HNV72411.1"
                     /translation="MRKFVLCCVMALAVSVPTAFAFDVSPTQTPGQKPAKMTPVQIAP
                     EALDALLENSSVAEGASMVTFSEDGKSFSSARGTLSEPLSGDLSEAGLNYILKNATLF
                     NVPVNKGKDLLKVVRNEEAAGASHVSYAMVLNGVPVYGALIELHIDKNRQVQLANGAL
                     PTIKEVTNQITIGRIEAIAAAKRAVGISKIRSVARAELAVKAESDNGRMVYVVKIAAA
                     EPLGDWEVLIDAETGKEISRLNQMMFADPVTGRGDVYPFNPLVSDVSEQDLPHLTTNT
                     LKGLYCNVVNDDGPTAVNTDNIHKYDPNDTHFDEANMYYYINQIHDFHKKLGATKMDK
                     PLKATVHYGDKYDNAYFSPWEDAMAFGDGNRLNDLAKEETVAYHEYSHATINTIVSLT
                     YSKESGAINEGQADYFACSLSNDT"
BASE COUNT          350 a          420 c          385 g          288 t
ORIGIN      
        1 gcgattcggc cccgtatcgt cttcggataa agggcatgct ccctgccgcg acggcgaatc
       61 gttgagcgat accaagggga agactcgtat gcggagaata cagtgacctt gattttgcat
      121 tccctggtct tgcgtacaca ggtcgatggt gataatctga gagggtaccg aacaatttcg
      181 taaggagtat ggaacatgag gaaattcgtt ctttgttgtg tgatggcgct tgcggtcagc
      241 gttccgacgg cttttgcctt cgacgtctcg ccgactcaga ctcccggcca gaagccggcg
      301 aaaatgaccc cggtgcagat cgcccccgaa gcgcttgatg ctcttctcga aaattcaagc
      361 gttgccgaag gcgccagcat ggtcactttc agcgaagacg gcaagagctt cagcagcgcc
      421 cgtggcaccc tctccgagcc cctttccggc gacctcagcg aagccggcct gaactatatt
      481 ctgaagaatg cgaccttgtt caatgtaccg gtgaacaagg gcaaagatct gctcaaagtg
      541 gttcgcaacg aagaagccgc cggtgccagc catgtctcct atgccatggt actgaacggc
      601 gttccggtgt atggcgccct gatcgaactg catatcgaca agaaccgtca ggtgcagctg
      661 gccaacggcg ctctgccaac cattaaggaa gtcacgaacc agatcaccat cggtcgcatc
      721 gaagccattg ccgcggccaa gcgcgctgtt ggcatctcga agattcgcag cgttgcccgt
      781 gccgaactcg cggtcaaagc cgagagtgat aatggtcgca tggtgtacgt ggtgaagatt
      841 gccgccgccg aacctctggg cgattgggaa gtcctgatcg acgccgaaac cggcaaagaa
      901 atctctcgtc tgaaccagat gatgttcgcc gacccggtca ccggccgcgg cgatgtctat
      961 ccgttcaacc cgctcgtcag cgatgtttcc gaacaggatc tgccgcacct gaccacgaac
     1021 accctgaagg ggctctattg caacgtggtg aatgacgacg gtccgaccgc cgtgaatacc
     1081 gacaacattc acaagtatga cccgaacgac acgcacttcg atgaagcgaa catgtactat
     1141 tacatcaacc agattcatga ctttcacaag aaactgggcg ccaccaagat ggacaaacct
     1201 ctcaaggcta cggttcatta cggcgacaaa tacgataacg cctacttcag cccatgggaa
     1261 gacgcgatgg ctttcggtga tggcaaccgc ctgaacgatc tggccaagga agaaaccgtt
     1321 gcgtatcacg aatactctca cgccacgatc aacaccatcg tcagcctgac ctactccaag
     1381 gaatcaggcg cgatcaacga aggtcaggcc gactacttcg cctgctcgct gtcgaatgac
     1441 acc
//
LOCUS       DAORHD010001402         1443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123593_length_1443_cov_2.360231, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001402 DAORHD010000000
VERSION     DAORHD010001402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123593_length_1443_cov_2.360231"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..318
                     /locus_tag="PKO06_22070"
     CDS             64..318
                     /locus_tag="PKO06_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017302020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HNV72412.1"
                     /translation="MKTVELQFPETVFSALRKDPQEFARELRLTAAVKWYEMGQISQE
                     KAAEIAGLSRFDFLAALVRFNVSPFQFNANELDEEVKNAL"
     gene            902..>1443
                     /locus_tag="PKO06_22075"
     CDS             902..>1443
                     /locus_tag="PKO06_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72413.1"
                     /translation="MQWSPEQVREIADQALQLVEARRRFSEAAQSSETARWNSPQYVW
                     VCLKNAVRDQVWENAPEDLRELHEAMATVLRRSNGFKRHEHLIGLGIWPNGMPPNTLD
                     LNEILHRLRTSFKAEYGFGRLGLKRGYLVRLVRQILTTVGGPISRPLLYLSVREFVQL
                     PRVTVGLPPQIEVGGSAHRG"
BASE COUNT          309 a          390 c          407 g          337 t
ORIGIN      
        1 gattctgagc cttcgtgcta ggatgatcac ggactacatc gaaccatcga ggagcgtttt
       61 ttcatgaaaa cggtcgagtt acagtttccc gagaccgtgt tctccgctct gcgcaaagat
      121 ccgcaggaat ttgcccgcga actacggctc actgccgcag tcaagtggta tgagatgggt
      181 cagatctcac aggagaaggc ggcggaaatc gccggactgt ctcgttttga ttttctcgcc
      241 gccctggttc gcttcaatgt ctcaccgttc cagttcaatg cgaacgagct tgacgaagaa
      301 gtgaaaaatg ccctttgaaa atgttgttgt caacgcctca cccctgatct gtctctgcag
      361 catctcgcac gagtggctat tacccagcct cttcaaacag gtgctggttc cgtccgcagt
      421 gcatgatgaa gtgacacgct ccgccgacaa acattcgtta gagccggcac tttctcggct
      481 agagcgatta actatcgttc cagaatgtgt cgtagcagat tccattcgaa actgggattt
      541 aggacgtggt gaaactcaag tgctgggctg tgcctacgga aaacccgacc tttgtgtcgc
      601 gctcgatgat gccgcagctc ggagatgcgc caagactcat tcgattccgg tcatcggaac
      661 cggtggcatt ctcattgcgt cgaaaaaacg gggactgata cgatctgtca gcgcatgtct
      721 cttttccttg aaaaccgctg gaatgtggct ttatgacgct cagttcaaaa tgttgctcga
      781 gatcgccgga gagtaaccac aatcgcagac aaccatgtct tctttagata gaactctttt
      841 ccggaagcgg ttggagcggt ttttgctacg ggcgtttccg gcgcgggagt tttgcggaag
      901 tgtgcaatgg tcgccggagc aggtgcgcga gatcgccgat caggctctgc agttggtcga
      961 ggccaggcga cgattctcgg aagcggcaca gtcttctgaa acggcgcgct ggaattcacc
     1021 gcaatatgtc tgggtatgcc tgaagaacgc ggtgcgcgac caggtctggg aaaacgcccc
     1081 ggaagatctt cgggaactgc acgaagccat ggcgaccgtc ttgcgcagga gcaacggctt
     1141 caaacgacac gagcatctga tcgggctcgg catctggccc aacggcatgc cccccaatac
     1201 cctcgatctc aacgagattt tgcatcggct gagaacctct tttaaagcgg aatacggttt
     1261 tggccgactc ggactcaagc gcggctacct ggtgcgcctg gtgcggcaga ttctgacgac
     1321 cgtcggtggt ccgatctctc gtcccctgct gtatctgagc gttcgcgagt tcgtgcaact
     1381 gccacgagta accgttgggt tgccgccgca gatcgaggtc ggcggctctg ctcacagagg
     1441 cat
//
LOCUS       DAORHD010001403         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123859_length_1441_cov_2.626984, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001403 DAORHD010000000
VERSION     DAORHD010001403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123859_length_1441_cov_2.626984"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKO06_22080"
     CDS             complement(<1..312)
                     /locus_tag="PKO06_22080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72414.1"
                     /translation="MQPRLKLFAGVMPGANTLFSALILSGMLLLGQGPIPSFGQLTGT
                     PFPGSPTPQPASGRPVASPAPAVPASGTPATPQTPSASGSMAHFDPQMDNHLSAPLRP
                     IG"
     gene            494..>1441
                     /locus_tag="PKO06_22085"
     CDS             494..>1441
                     /locus_tag="PKO06_22085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI/YhbO family deglycase/protease"
                     /protein_id="HNV72415.1"
                     /translation="MDLKNKMIAILIAPKYQDDEGVAPAKYLRELGADVRYVGIQKGS
                     CPGKGQAMVQVETTVNEVQASNFDGLVIPGGAAPETLRLHKDALAFVKGFFDEAKPVA
                     AICHGPQVLMSAGLARGRTMTCYQGIRDDLMNAGAQYLDQEVVIDGNLVTSRVPQDLL
                     AFNKAFAELLVKYDREKTPWAHASPAQVLEFAIGNEIKAYDVYDSLAKCAKDKVAKAK
                     FKFLAETERGHREKLEELYMKLYGGRKPEPREMGERGGEGHLDVDASDDLLKILTLAV
                     NAEEAAHSMYAQIAEKTRHAEAKKLFQTLAEEELEHRRLL"
BASE COUNT          334 a          372 c          460 g          275 t
ORIGIN      
        1 cccaattgga cgcaggggcg cggagagatg attgtccatc tgcgggtcga aatgcgccat
       61 ggaaccgctc gcagagggcg tttgtggcgt tgccggggtt ccggaagccg gcacggcagg
      121 tgccggactc gctaccggcc tgccgctcgc tggttgcgga gtaggactcc cagggaacgg
      181 ggttccggtc aactgcccga acgaggggat ggggccttgc cccagcagga gcatcccgga
      241 cagtatcaga gcggagaaca aggtgttcgc tccaggcatc acgccggcga acagcttgag
      301 acggggttgc atgatggtat catagcagaa tgttcccgtt cgtacaagac agaaagagct
      361 cgtgcctgaa gaagcgcaga acgaaatgac ccttgatcgc cgctggcgat tttgacacgg
      421 tttcaaggca cgtgctagat tgactgtgtt cgcataccat cgaaggaggg aagcggtttt
      481 ccgcgactaa cccatggatc ttaaaaacaa gatgatcgcg attctcattg ccccgaagta
      541 tcaggatgac gaaggggtgg caccggcgaa gtatctgcgc gaactcggtg cagatgtccg
      601 gtatgtcgga atccagaagg ggtcgtgccc aggaaagggg caggcgatgg tgcaggtcga
      661 aacgacggtg aacgaggtgc aagcatcaaa ttttgatggg ctcgtgattc ccggtggggc
      721 tgctcccgag actctgcggt tgcacaagga cgcgcttgct ttcgtgaaag gctttttcga
      781 cgaagccaaa cccgtggcgg ctatctgtca tgggccacag gtcctgatgt cagcaggtct
      841 cgcgcgcggg cgaaccatga cctgctatca gggaatccgg gacgacctca tgaatgcggg
      901 tgcgcaatac cttgatcagg aagttgtcat cgacggaaat ctcgtcacct cgcgggtacc
      961 gcaggatctc ctcgccttca acaaggcttt tgccgagttg ctggtgaagt atgatcgtga
     1021 gaagactccc tgggcgcatg ccagcccggc ccaggtgctg gagttcgcca tcggcaatga
     1081 aatcaaggca tacgatgtct atgattcgct ggccaagtgc gcaaaagaca aagttgccaa
     1141 agcgaaattc aagtttctgg ccgagaccga gcgtgggcat cgtgagaaac tcgaagagct
     1201 gtacatgaag ctgtacggcg gtcgtaaacc cgaaccgcgc gaaatgggcg agcgcggggg
     1261 cgaagggcat ctcgacgtcg atgcttcaga cgatctgttg aagattctga ccctggcggt
     1321 caatgccgaa gaagccgctc attccatgta tgcgcagatt gccgaaaaga ctcgccacgc
     1381 cgaagccaag aagctgtttc agacgctggc cgaagaagaa ctggaacatc gccgactgct
     1441 g
//
LOCUS       DAORHD010001404         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_123986_length_1440_cov_3.427437, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001404 DAORHD010000000
VERSION     DAORHD010001404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123986_length_1440_cov_3.427437"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1352
                     /locus_tag="PKO06_22090"
     CDS             69..1352
                     /locus_tag="PKO06_22090"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HNV72416.1"
                     /translation="MPQQRTLARQVQCEGVGLHTGNRSKLSAHPAPAGHGVVFRRTDI
                     AGSPEIPARMEFVHEVSRGTTLQKDGVKVHTVEHVVAALFGLQIDNVLIELHGDEPPA
                     TDGSALPFVKLFQEAGIQELDAEAQYCSVRSTFSVADGQKTISYIPADNFQVSFTLAY
                     PEPVPISQHLHVCVSPDSFAQEIAFSRTFGFEYEFDMLKERRLALGGSLENAVVIRRD
                     GGIMNPEGLRSETEFVRHKILDLIGDLALIGRRVKGHVVACRTGHAYNVRFARKLCEL
                     HHREQERTAGPMMNVEAIKEILPHRYPFLLVDRILSIEAGKSAVGYKNVTGNEEFFNG
                     HFPQRPVMPGVLICEAMAQVAGVLFLSQPEHKGKLPFFVGIDKIRFRKPVTPGDRLEM
                     YVQTLKVRGNTGRVAVEAKVDGELVCDGELMFTIL"
BASE COUNT          314 a          369 c          436 g          321 t
ORIGIN      
        1 catcgcgaag gcaaaggaat cacgaaacag gccatctatc gcctggtata cgtgaaaggg
       61 ttctaggtat gccgcaacaa cgaacgttgg cgcgtcaggt gcagtgtgaa ggcgtggggc
      121 ttcacacggg caatcggtcg aagttgtccg cgcatccggc tccggcgggc cacggcgtgg
      181 tttttcgccg caccgacatc gcgggcagtc ccgagattcc ggctcgcatg gagtttgtgc
      241 atgaggtctc tcgcgggacg acgttgcaga aagatggagt caaggtgcat accgtcgagc
      301 atgtggttgc cgcgctgttt ggtctgcaga tcgacaatgt cttgatcgaa ctgcatgggg
      361 atgaaccccc cgcgacggat ggcagtgccc tgccatttgt gaaactgttt caggaagcgg
      421 ggattcagga actggacgcg gaagcccagt attgttccgt gagatcgacg ttttctgtcg
      481 ccgatggcca aaagacgatt tcctacattc cggcagacaa ttttcaggtc tcttttacgc
      541 tggcctaccc tgaaccggtg ccgatttcgc agcacctgca cgtgtgcgtg tctcccgatt
      601 cgtttgcaca ggagattgcc ttttctcgaa ccttcgggtt tgagtatgag tttgacatgt
      661 tgaaagagcg ccggttggcg ttaggaggat cactcgaaaa cgcggtggtg atccgcagag
      721 atggcggaat catgaatccc gagggacttc gttccgagac ggagtttgtt cggcacaaga
      781 tcctggatct gattggggac ctcgctctca tcggtcgccg agtgaaagga catgtggttg
      841 catgccgcac cggccacgcg tataatgtcc gcttcgccag aaagttgtgc gagttacacc
      901 accgggaaca ggaaaggaca gccggaccca tgatgaacgt cgaagccatc aaggaaattc
      961 tgccgcatcg ctacccattt ctgctcgttg accgcattct ctcgatcgag gcgggcaagt
     1021 cggcagtcgg atacaagaac gtgacgggga atgaagagtt tttcaacggt cattttccgc
     1081 aacgaccggt gatgccgggc gttctgatct gcgaggcgat ggctcaagtt gctggtgtgc
     1141 tttttctctc gcagcctgaa cacaagggaa aattgccgtt ctttgtgggc attgataaaa
     1201 ttcgctttcg caagcccgtc actccgggag atcgccttga gatgtacgtc cagacgctga
     1261 aagtgcgtgg gaacaccggt cgcgtggccg tagaagccaa agtggacggc gagctggtgt
     1321 gtgacggtga gctgatgttc acgattttgt aaggcgacag ggatagaccc atgaaaattc
     1381 atcagacagc catcgtttcc cccgaagcca agatcgaccc gaccaagccc gacggcacgc
//
LOCUS       DAORHD010001405         1440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124013_length_1440_cov_2.892419, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001405 DAORHD010000000
VERSION     DAORHD010001405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124013_length_1440_cov_2.892419"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKO06_22095"
     CDS             <1..583
                     /locus_tag="PKO06_22095"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV72417.1"
                     /translation="NLSYLVEAKRKRRSPLPELWFHFIINRYNEGEMEEYVNLVDRLT
                     SPVKHLFPPIIFWTNLLAFDEVADLVVAIPEQRREGILQKCQERGIYAIWNENVSHDK
                     SMTQCTKWTEPFILVSGHLQPCCALNEANQRRYQEEHAFINVFEEDFHQFWDGDRLKN
                     FVRTLQSGGINEVCRYCHIFNHPGIWRGLRRKM"
     gene            600..>1440
                     /locus_tag="PKO06_22100"
     CDS             600..>1440
                     /locus_tag="PKO06_22100"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV72418.1"
                     /translation="MRVQLLTPPKSSTAYVDFSVSPLGLMYLAASVRQGFPACEVKIT
                     DGQFHDESSMLRDITMFAPNIIGISSLTSVVSAGYRLAREIKKINPTTLVVFGGVHAS
                     CLPEEVISSPEVDVVVVGEGETAFAQIVHKVARGDHDFSDIDGIWYRTSAGVRKTASR
                     KAINSIDVIPFPARDLVDLKKYTYGPFSAAQGVVDGILMSRGCPCQCLFCANRVWRMA
                     RPRFRLRSPRNIADELEEMKNRYGVTRFENQSDVFGIPERWSIEVCEEMVRRNLNLPW
                     ITQV"
BASE COUNT          344 a          333 c          406 g          357 t
ORIGIN      
        1 aaatctgtcc tatcttgtcg aggcgaaacg taagcgtcga tcacctcttc ccgagttgtg
       61 gtttcatttc atcatcaacc gatataacga gggcgagatg gaagagtatg tgaatctcgt
      121 tgatcgcttg accagcccgg tcaagcacct ctttccgccg atcatcttct ggacaaacct
      181 gctggcgttt gatgaagtcg cagatctggt cgtggcgatc cccgagcaac gaagagaagg
      241 cattcttcag aagtgccagg aacgcggtat ttatgcgatc tggaatgaaa atgtgtctca
      301 tgacaagagc atgacgcagt gcaccaaatg gacagaaccg ttcattctcg tctcaggaca
      361 tcttcagcct tgttgtgcgc tcaatgaagc gaatcagcgc agataccagg aagagcatgc
      421 attcatcaat gtgttcgagg aagacttcca tcagttctgg gatggagatc gactgaaaaa
      481 cttcgtgcga acgctgcaga gcggcggtat caatgaggtc tgccgctatt gtcacatttt
      541 caaccaccca gggatttggc gtgggttacg gcgaaagatg taggggtggt tcaagccaga
      601 tgcgtgttca acttctgacg ccgccaaaga gttcgacagc gtatgtcgac ttttccgtgt
      661 cgccgcttgg attgatgtat ctggctgcga gtgtcagaca gggcttccct gcatgtgagg
      721 tgaaaatcac tgacggtcag tttcacgacg aaagcagcat gctgcgtgac attacgatgt
      781 ttgcaccgaa tatcattggt atttcgtcgc tgacttcagt ggtctcggcg ggctaccgtc
      841 tggcccgtga gataaaaaaa atcaacccga ccacactggt ggtctttggc ggtgtgcatg
      901 cgtcttgcct gcctgaggaa gtcatcagtt ctccggaagt tgatgtcgtt gttgttggag
      961 agggtgagac ggctttcgcg cagatagtac acaaagttgc gcgaggtgac catgatttct
     1021 cggatatcga cggcatttgg tatcgtacga gtgcaggcgt tcgaaaaacg gcttccagaa
     1081 aagcgattaa cagtattgat gtgattcctt ttcccgcccg cgatctggtc gatctcaaga
     1141 aatacacgta cggaccgttc agcgcagcgc agggtgtcgt tgatggcatt ctgatgtcgc
     1201 gagggtgtcc gtgccagtgc ctgttttgtg ccaacagagt ctggagaatg gcccgcccgc
     1261 gattccgttt acgcagcccg cgaaacattg ctgacgaact cgaagaaatg aagaatagat
     1321 acggtgttac cagattcgag aatcagagcg atgttttcgg aatcccggag agatggtcga
     1381 ttgaagtctg cgaggaaatg gttcgacgga atctgaatct gccgtggatc acgcaggtca
//
LOCUS       DAORHD010001406         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124281_length_1438_cov_4.478670, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001406 DAORHD010000000
VERSION     DAORHD010001406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124281_length_1438_cov_4.478670"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PKO06_22105"
     CDS             <1..290
                     /locus_tag="PKO06_22105"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72419.1"
                     /translation="RLLKDLFARGQNDKIITFTLGNIADKRNDLQTAIEWWTRTMSQD
                     SQHYEALFNIGVAYYKLSERERAVQCWKRVLDLNPDLETRQIVLEALKQVE"
     gene            332..1312
                     /gene="trpS"
                     /locus_tag="PKO06_22110"
     CDS             332..1312
                     /gene="trpS"
                     /locus_tag="PKO06_22110"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV72420.1"
                     /translation="MKKRLVSGMRTTGSLHLGHYEGTLKNWVRLQEQYECVFFAAVWH
                     AFTDRLKIDDLPRVIHDMVIDWLSVGIDPKKSIIVRQSAMPQHAELCTILGMFSPLGW
                     LERCPTFKEEIRGEDSRENLSLGKLMYPVLQTADIAVYRGEAVPVGKDQLPHLELARE
                     IIRRFNFHYGEVLPEPKEVLSEVPMLLGIDNRKMSKSYNNSIELRETPESLKQKVHLM
                     ITDPARVRRNDPGNPDICTVYSYHRLYTNDRLEEIGSGCRSAGIGCRDCKTIIYERLN
                     TLLEGFRAKRAELEKTPGLVKDILADGEARGRVLADATIDEIRRRMGFVG"
     gene            1316..>1438
                     /locus_tag="PKO06_22115"
     CDS             1316..>1438
                     /locus_tag="PKO06_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNV72421.1"
                     /translation="MGAYQVRLPVFEGPFDLLFHLIEDQKVNIFDIPIAQIAGQY"
BASE COUNT          327 a          408 c          403 g          300 t
ORIGIN      
        1 agcgcctgct gaaagacctg ttcgcccgcg gtcagaacga caagatcatc accttcacgc
       61 tgggcaatat tgccgacaag cggaacgatc tgcagactgc catcgagtgg tggacgagaa
      121 ccatgagcca ggattcccag cattacgaag cactgttcaa catcggcgtg gcctattaca
      181 agctgagtga acgtgaacga gcggtccaat gctggaaacg ggttctcgat ctcaaccccg
      241 atctcgaaac ccgacagatc gtgcttgaag ctctgaagca ggtagagtag aattctccct
      301 cccaattctt ccaggagacc tcagaccaat catgaagaaa cgacttgtca gcggtatgcg
      361 caccaccgga tccctgcacc tgggccacta tgaaggcacg ttgaaaaact gggtgcgact
      421 gcaggaacag tacgaatgtg tgttctttgc agcggtgtgg cacgctttca cggaccgcct
      481 gaagatcgac gacctgcctc gggtcattca tgacatggtc attgactggc tttcggtcgg
      541 cattgatccg aaaaagtcga tcatcgttcg ccagtcggcg atgccgcagc atgccgaact
      601 ctgcacgatt ctgggcatgt tttcaccact cggctggctc gaacgctgcc ccaccttcaa
      661 ggaagaaatt cgtggtgaag acagtcgcga gaatctttcg ctgggcaagc tgatgtatcc
      721 ggtgctgcag actgccgata tcgccgtcta tcgcggtgag gctgtccccg ttggcaaaga
      781 ccagctgccg catctcgagc tggcgcgcga gatcattcgt cgcttcaact ttcactacgg
      841 cgaagtgctg cccgaaccga aagaagtgtt gtcagaggtg ccgatgctgc ttggcatcga
      901 taatcgcaag atgagcaagt catacaataa ctcgatcgag ctgcgcgaaa ctcccgagtc
      961 gctgaagcag aaggtgcatc tgatgatcac cgacccggcc cgcgtgcgcc ggaacgatcc
     1021 gggcaacccg gacatctgca ccgtctattc ctatcatcgc ctctacacca acgatcgtct
     1081 cgaggagatc ggctcagggt gccggtcagc tggtatcggc tgtcgcgact gcaagacgat
     1141 catctatgaa cggttgaaca cgttgctcga ggggttccgg gccaagcgag ccgagctcga
     1201 gaagaccccc ggtctggtca aagatattct ggccgacggt gaggcgcgcg gtcgcgtgct
     1261 cgccgacgcc acgatcgacg aaattcgccg ccgcatgggg tttgtagggt aaaacatggg
     1321 tgcgtatcag gttcgtctgc cggttttcga aggtccgttc gatctgctgt ttcacctgat
     1381 cgaagaccag aaggtcaaca tcttcgatat tccgatcgcc cagatcgccg gtcagtac
//
LOCUS       DAORHD010001407         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124290_length_1438_cov_3.872017, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001407 DAORHD010000000
VERSION     DAORHD010001407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124290_length_1438_cov_3.872017"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="nagB"
                     /locus_tag="PKO06_22120"
     CDS             complement(<1..642)
                     /gene="nagB"
                     /locus_tag="PKO06_22120"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HNV72422.1"
                     /translation="MEVIVKPTQEAACKLAARIVARFMRQKPNLVLGCATGSSPLPLY
                     AELVRMHREEQLSFRSVTSFNLDEYVGLDPRHPASFRACMEEHLFRHVDFAPGATHLP
                     DGCATDLAAACESYEQAIRRAGGIDLQILGIGGDGHLAFNEPTSSLRSRTRLKTLTAH
                     TRQANASAFGSPDKVPGHVLTMGLGTIMEARCCLLLGFGASKAAAIRDMIEGPV"
     gene            722..>1438
                     /locus_tag="PKO06_22125"
     CDS             722..>1438
                     /locus_tag="PKO06_22125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV72423.1"
                     /translation="MNQKTSGNPWLFVPALFFLEGIPYFIVSTISVTMFKKLGVANDQ
                     IGLWTSLITWPWIVKMLWGPLVESTSTKRKWILGTQIAAIVTMALVSLTITSENFLYP
                     TLVVLTVLAFLSATHDIAADGFYLLALAPEDQAYFVGIRSTAYRLANVFCNGGLVYLA
                     GVFENTPGVAVAEAWRNIILIGAGVYLLFVVLGYSAMPKPRQDKDRVESFPFREAFVE
                     YFSQSKLAVILGFILTYRFGE"
BASE COUNT          277 a          419 c          426 g          316 t
ORIGIN      
        1 gaccggccct tcgatcatgt cgcgaatcgc cgccgctttg ctcgcaccga agccaagcag
       61 cagacagcag cgggcttcca tgatggttcc gaggcccatg gtcaggacgt gcccgggcac
      121 tttgtccggg ctgccgaagg cggaagcatt tgcctgacgc gtgtgtgcgg tgagcgtttt
      181 cagccgcgtg cgtgaacgaa gcgacgaggt gggctcattg aacgccagat gcccgtcacc
      241 gccgatcccg agaatctgca ggtcgatgcc gccggcccgg cggatcgcct gttcatagct
      301 ctcgcaggcg gctgccagat cggtggcgca gccgtcgggc aggtgcgtgg cgccgggggc
      361 aaaatcgaca tgacgaaaca gatgctcttc catgcaggcg cgaaacgacg caggatgcct
      421 gggatctagc ccgacatact catcgagatt gaacgaggta accgagcgaa agctgagttg
      481 ttcttcgcga tgcatgcgta ccagttccgc atacagcggc agaggtgatg agccggtcgc
      541 gcagccgaga accaggttag gtttctgacg catgaaccgg gcgacaatgc gcgcggccag
      601 cttgcatgcg gcttcctgag tgggttttac aatgacttcc atgacttttc tccgtatggg
      661 gtcgccaaga tgacgcggat attctatcat caggcatcgc gcgaaaggaa aacaaccttc
      721 tgtgaaccag aaaacgtccg gcaatccctg gctgttcgtg cccgccctgt tctttctcga
      781 gggcattccc tacttcattg tcagtaccat ttcggtcacc atgttcaaga aactgggagt
      841 ggccaacgat cagatcggcc tctggaccag cctcatcacc tggccctgga tcgtgaaaat
      901 gctgtggggt cccctggtcg aatcgacctc caccaagcga aaatggattc tcggcacaca
      961 gatcgcggcg attgtcacca tggccctggt ttcattgact atcaccagcg agaactttct
     1021 ctatccgaca ctcgtcgtgt tgaccgtgct ggcgtttctg tcggcgaccc atgacattgc
     1081 cgcagacggg ttttaccttc tggccctggc tccggaagat caggcctact tcgtgggcat
     1141 tcgcagcacg gcctatcgcc tggcgaacgt cttctgcaat ggcggtctgg tctatcttgc
     1201 cggggtcttc gagaacaccc cgggagtggc cgtggcagag gcctggcgta acatcattct
     1261 gatcggcgcc ggagtctacc ttttgttcgt ggtactcggg tattcagcga tgccaaaacc
     1321 gcgtcaggac aaggatcggg tcgagtcctt tcccttccgc gaagcgttcg tcgagtattt
     1381 ttcacagtcg aagctggccg tcattctcgg gttcatcctg acgtatcggt tcggcgag
//
LOCUS       DAORHD010001408         1438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124299_length_1438_cov_3.561822, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001408 DAORHD010000000
VERSION     DAORHD010001408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124299_length_1438_cov_3.561822"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PKO06_22130"
     CDS             complement(<1..847)
                     /locus_tag="PKO06_22130"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV72424.1"
                     /translation="MTQPWFNAWVERKQKYAQTDELFLPCDRFASLCVGNGLPPVVQK
                     STFSFRNVKDGASRFLGKSKSGEKPYARIYTRLGNPTTEYLEKVLFHLDCQHIIDAAL
                     AADERKPTIGSLVFASGMGAIASTTFALVKSGDEIISGSVYGCTDSFFRSLEKFGITA
                     RFVDTSSLKAVEQALAESPRAAMVFLESPDNPTLRVSDIAAISRLTQKRGIPLVVDNT
                     FATPYLQQPFRLGADIAIQSLTKYVNGHSTSVAGVVIGPWQFVAEHLFPVAKDHGPTP
                     SPFDSW"
     gene            1067..>1438
                     /locus_tag="PKO06_22135"
     CDS             1067..>1438
                     /locus_tag="PKO06_22135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72425.1"
                     /translation="MRSRYMLWGVAGLMTLGAWWLASPARAVTDSPMRQILEKAFFSE
                     RLIKDSLYPKSTKDVYAIPKRQFVHGLPRINKPFIYEPDRYTWELAEEEHNKKLLAQA
                     PAQNLLPGTVQPLPMAPTPLPP"
BASE COUNT          316 a          436 c          389 g          297 t
ORIGIN      
        1 accaggagtc aaaggggctc ggcgtcggac cgtggtcttt cgccaccgga aacaggtgtt
       61 cggcgacaaa ctgccacggg ccaatgacca ccccggccac tgaagtcgag tggccattca
      121 catacttggt caggctctga atggcgatat ccgcccccag gcggaacggc tgctgcagat
      181 acggcgtggc aaacgtgttg tcgaccacca gcggaatgcc gcgcttctgc gtcagacggc
      241 tgatggcggc gatatcggaa acccgcagcg tcggattgtc gggcgattcg agaaatacca
      301 tggcagcgcg gggcgattcg gccaacgcct gttcgacggc cttcaacgaa gaggtatcca
      361 caaaacgcgc cgtgataccg aatttctcaa gcgaacgaaa gaaggaatcg gtgcagccgt
      421 agacgctgcc gctgatgatc tcgtctcctg atttcaccag ggcaaatgtc gtcgaggcga
      481 tcgcccccat tcctgaagcg aacaccagcg agccgatcgt cggtttccgt tcgtcagcgg
      541 caagagcggc atcaatgatg tgctgacaat cgaggtggaa gagaaccttc tcgagatact
      601 cggtcgtcgg attccccaag cgggtgtaga tgcgcgcgta gggtttctca ccactcttcg
      661 acttgccaag aaagcgactg gccccgtctt tcacgttccg aaagctgaac gtcgacttct
      721 gcacgaccgg cggaagccca tttccaacac agagcgaggc aaatcggtca cagggcagaa
      781 agagctcatc ggtctgagca tacttctgtt tgcgctccac ccaggcgttg aaccacggtt
      841 gcgtcacaat cggtctcctt gaggttttct cgtgcgccgt ttcgcgcaga tcaaaacggc
      901 cgaaccccgg ccatgtggtc aggatatcgt catcttctga tgttaccatt acccctgtca
      961 catcacgaag cgttttttgt acacttttcc cttgacactt ctcgcgcagg tgaacacatt
     1021 ctcgcgccat cctgcactta ctttgttcgg tcaaggagga cgtgtcatga gatcgcgcta
     1081 catgctctgg ggagttgccg gcttgatgac cctgggtgcc tggtggctgg cttcgcctgc
     1141 gcgggctgtc accgattcac ccatgcgtca gattctggaa aaagccttct tcagcgagag
     1201 gctgatcaag gattcgctct atcccaaatc cacaaaggat gtctacgcca tcccgaaacg
     1261 tcagttcgtg cacggcctcc cacggatcaa caaacccttc atttacgagc cagaccggta
     1321 cacgtgggaa ctcgcggaag aagaacacaa taagaaactg ctggctcagg cacctgctca
     1381 gaacctctta cccggcacgg tccaaccgct gccaatggct cccactccgc tgcccccc
//
LOCUS       DAORHD010001409         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124734_length_1435_cov_4.124638, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001409 DAORHD010000000
VERSION     DAORHD010001409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124734_length_1435_cov_4.124638"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PKO06_22140"
     CDS             complement(<1..394)
                     /locus_tag="PKO06_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72426.1"
                     /translation="MRCTGITCRNRRGTAFLLAMALLVVGGMVASQLVPNEVTIVQRR
                     QEQDLKHQLGKLRMAIQLERIASFSPLYEADWSNRAQFLQYLDALIDRHLIPSIPADP
                     LTPVFQWGTGTGKIYWIPTLNYLASSSFE"
     gene            complement(665..>1435)
                     /locus_tag="PKO06_22145"
     CDS             complement(665..>1435)
                     /locus_tag="PKO06_22145"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV72427.1"
                     /translation="LLSMAVSPQGDIGLLDSSSGRVHVLSATGTLRFSTPAELPGVEP
                     LLKPQGLAFGDDGEIFVADTGNQRVVQFTPRGEVRLLGQAGIFSGQFTLPTALVWSDG
                     YLYVLDSALCRVNLFAPDGTFVASTGGRGTHPGRFRLPTSLAIDETGQLLVIDSLLPG
                     VTRFDSQLTLLQASRLETGTSGFPLSRAAVVGNGLTFYLSKQRQELLVFFENTFIALL
                     QPSSLNLADVFWTDICARENRLYLLDARRARVDLFELE"
BASE COUNT          358 a          389 c          404 g          284 t
ORIGIN      
        1 cttcgaaact cgaagaagcg agataattga gcgtgggaat ccagtagatt tttcccgtcc
       61 cggtacccca ctgaaacaca ggagtcagtg ggtcggcagg gatggacggg atcaggtggc
      121 gatcaatcag tgcatcgaga tactgcagaa attgtgcgcg attcgaccag tcagcctcat
      181 agagtgggga aaaactcgcg attctctcga gttgtatggc catgcgcagc ttcccgagct
      241 ggtgcttgag atcctgctcc tgacgacgtt gtacgatggt cacctcgttg ggaacgagtt
      301 gagaagccac cataccgccg acaacaagca gagccatcgc cagaagaaag gccgttcctc
      361 tgcggttacg acaggtgatt ccggtacagc gcatcctgat gttcctcttg tccttgaaac
      421 ctcacggaaa ttatgtctga agacctgaac aacaacatac tagaagccaa cattgatgtc
      481 aacggaaata ggaaggcttc cgtgattctc aaactgaaaa tcctgggtcc agaatgggaa
      541 gaactgaaac gcctgaacgg cgatctcatc gtcttcgcga cagagttatt cccactgtat
      601 ctccggtctg ctgctgtttt cgggctatgg gttcagcttc gaaacacgtc tgacctttct
      661 gagatcactc gagctcaaac aggtcgacgc gagcgcgacg cgcatcgagc agatagagac
      721 ggttttctcg tgcgcagatg tcggtccaga agacgtcggc aagattcaaa gagcttggct
      781 gcaagagtgc aatgaacgtg ttctcgaaaa acacgagaag ctcctggcgc tgtttcgaaa
      841 ggtagaacgt cagcccgttg cccaccacgg ctgcacgact gagaggaaac ccggacgttc
      901 cggtctcgag gcgacttgcc tgcagaagcg tgagctggct gtcaaagcgc gtgacaccgg
      961 gaaggagact gtcgatgacc aacagttgcc ccgtttcatc gattgccaga cttgttggca
     1021 gccggaacct accggggtga gtaccgcgtc ctccggtcga tgccacgaag gttccatccg
     1081 gagcgaacaa gttcacacga cacagcgcgc tatcgaggac atagagatat ccatcactcc
     1141 agaccagcgc ggtcggcagt gtgaactgcc cgctgaagat cccggcttgt ccaagcaggc
     1201 gcacttcccc gcgtggggtg aactgaacca cacgctgatt gccggtatcc gcgacgaaga
     1261 tctcaccgtc gtcaccaaag gccaagccct ggggtttgag caacggctcg acgccgggta
     1321 actcggcggg agtcgagaaa cgcaatgtcc cagttgctga cagaacatgc acacgtccag
     1381 agctggaatc gagaagtccg atgtctccct gaggtgagac agccatcgaa agcaa
//
LOCUS       DAORHD010001410         1435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_124736_length_1435_cov_4.110145, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001410 DAORHD010000000
VERSION     DAORHD010001410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124736_length_1435_cov_4.110145"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKO06_22150"
     CDS             complement(<1..260)
                     /locus_tag="PKO06_22150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72428.1"
                     /translation="MSADLPPTTQKPAEHWDEPRIVVFLIGLCLIIEAALIYLQAGPS
                     MAMVSGVTGVSGCTLLLFAWLVPLEEEDKQPTVTARGEHKPES"
     gene            complement(286..>1435)
                     /locus_tag="PKO06_22155"
     CDS             complement(286..>1435)
                     /locus_tag="PKO06_22155"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72429.1"
                     /translation="KKACKREIDAAEKRRQAAADRKDRAATEVEQLSKSLLEAEAEIA
                     DEQIRFQELDEQRRILEQALASVQDEYRKGSRELENLDHTYQSRAKIEDSTKNKLSEL
                     EVKIAEIKTHIQNKEAILTGDFGFQIGEPGQGSSRRWESREQILLHLTERKATREALE
                     PVNPLAIEDYEKAKERYDFLNSQVKDLTEAANSLEQVITEIEKISLERFRETYDKINL
                     VFADLFKILFPGGNSCLRLTDPEHPLESNVDIVCQLPGKKQMSLELCSGGEQSMISIA
                     LLFAILQVKPPAFCLLDEVEAALDEANVRRFTRLLRTFADKTQFLVITHNKETMQAVD
                     VIYGVTLEKTGISRPISIRLEDQDKIREFTVNKRHPAATAPQAMAAHS"
BASE COUNT          258 a          394 c          419 g          364 t
ORIGIN      
        1 gattctggtt tatgttcgcc tcttgctgtg actgtgggct gtttatcctc ttcttcaagt
       61 gggacaagcc aggcgaacag caggagtgtg catcccgaga cgccggtgac tccagagacc
      121 atggccatgc tgggaccggc ctgcagatat atgagggccg cttcaatgat cagacacagg
      181 ccgatcagaa agacgacgat gcgcggttcg tcccagtgtt ccgcaggttt ctgagtggtg
      241 gggggcagat cggcgctcat gcaggctcct cctcgttgtt cgagctcacg aatgggcagc
      301 catggcttga ggagccgttg ccgccggatg tcgtttgttc accgtgaatt cacggatctt
      361 gtcctggtct tcgagacgaa tgctgattgg ccgtgaaatg cccgtctttt caagcgtgac
      421 gccgtagatg acgtcgacgg cctgcatggt ctctttattg tgcgtgatga cgagaaactg
      481 ggtcttgtcc gcaaacgtgc gcaacaggcg cgtgaatcgc cgcacgttgg cttcatcgag
      541 tgccgcctcg acctcatcga gcagacagaa agccggcggt ttgacctgca gaatcgcgaa
      601 cagcagggcg atcgaaatca tcgactgttc accgcccgaa cagagttcga gcgacatctg
      661 cttcttgccg ggcagctggc acacgatatc gacgttactt tcgagcgggt gttccggatc
      721 ggtcaggcgc aggcagctgt tcccgcccgg aaagaggatc ttgaagaggt cggcaaacac
      781 cagattgatc ttgtcatagg tttcgcggaa tcgttcgaga gaaatctttt caatctcggt
      841 gatgacctgt tcgagcgagt tcgcggcttc ggtcaagtct ttcacctgac tgttcagaaa
      901 gtcgtatcgc tctttggcct tctcgtagtc ttcgatggca agcggattca ccggttcgag
      961 cgcttcgcgg gtcgccttgc gctcggtcag gtgcagcagg atctgttcgc ggctttccca
     1021 acgacggctg ctcccctgcc ccggttcacc gatctgaaag ccgaagtcac cggtcaggat
     1081 cgcttctttg ttctgaatat gcgtcttgat ctcagcaatc ttcacttcga gctcggacaa
     1141 tttgtttttc gttgagtctt cgattttggc acgactctga tacgtgtggt cgagattctc
     1201 gagttcacgc gagcctttgc gatattcatc ctgtacgctc gccaaggcct gctcgagaat
     1261 acgtcgttgc tcgtcgagtt cctgaaagcg aatctgttcg tcggcaatct cggcttcggc
     1321 ctcgagcagc gacttcgaga gttgctctac ctcggtggcg gcgcgatctt tgcgatccgc
     1381 ggcggcctgg cgccgtttct cggcggcatc gatctcgcgc ttgcaggcct tcttg
//
LOCUS       DAORHD010001411         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_125117_length_1433_cov_2.489840, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001411 DAORHD010000000
VERSION     DAORHD010001411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125117_length_1433_cov_2.489840"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PKO06_22160"
     CDS             <1..719
                     /locus_tag="PKO06_22160"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNV72430.1"
                     /translation="NRGGLPQVYGSDDYAEELKYYVGTYLQEEIQAEALSRNMGAFAE
                     FLEVIALSNGQEINYESLARDCGTSPGTLKNYLQILEDTMLGFSLPGYQKTRKRKAIA
                     RAKHYLFDLGVTNQLCRRGEIRERSELFGSAFEHFLVLELRAYLHYARKNLELNYWRS
                     TSQFEVDVVIGEELAIEVKGANLIQDKHLKGLRALKEEGLHRRYLVVSLDRQKRKTSD
                     DIEIWPWNAFLHELWAGRLL"
     gene            complement(1205..>1433)
                     /locus_tag="PKO06_22165"
     CDS             complement(1205..>1433)
                     /locus_tag="PKO06_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72431.1"
                     /translation="RAAYQKHAILKPKYANDLLHIAIATYANADLLVSWNFKHIVRFD
                     KILLYNSVNLEMGYRQIQIYSPREVTKVEKD"
BASE COUNT          337 a          352 c          368 g          376 t
ORIGIN      
        1 tgaaccgcgg aggtttgccg caggtgtatg ggtctgacga ctatgccgaa gaattgaaat
       61 actatgttgg aacctacctg caggaggaga ttcaagctga agcgctttcg cgtaacatgg
      121 gcgcttttgc tgagtttctc gaggtgattg ccttgtcgaa cggccaggaa atcaattacg
      181 aaagtctggc gcgtgattgc ggaacatcgc cggggacgtt gaaaaactac ctgcagattc
      241 tcgaagacac gatgcttggt ttttccctgc ccggatatca gaaaacccga aaaagaaagg
      301 ctattgctcg cgcaaagcat tatcttttcg atctcggcgt cacaaatcag ttgtgtcgcc
      361 gcggagagat tcgcgaacgg agcgaactgt tcggaagcgc ttttgaacac ttccttgtgc
      421 tcgaactgcg agcgtatctg cactatgccc gaaagaatct cgagttgaac tactggcggt
      481 cgacctctca attcgaggtt gacgtggtca tcggtgagga attggccatc gaagtcaagg
      541 gagcaaacct gatccaggat aaacacctga aggggttacg cgccttgaaa gaggagggtc
      601 tgcaccgaag gtatctggtc gtttccctcg atcgacagaa gcgaaaaaca tcagacgaca
      661 tcgagatctg gccgtggaac gccttcttgc acgagttgtg ggccggcagg cttctctgag
      721 actcttcgag ccatttcaca cggcgacgaa ctccacatcg ccctgtcaga ccctcgaacc
      781 gacggtgatc acgaggaagt gcctttcagc gttttctcga tgacctcgat gccgtgcgtg
      841 gttttgccgg gcgaaagttc aggttttcat gaacgacgga cttctcctgg tcgcaatccc
      901 catttgcgtc gtaaactcac tcggactgtt ggcttgaaca gagattgcgc tgaggaggtc
      961 gcaccggccc ccgccacggg tgaccagatg ccgatcgacg tagtcgatgg cccatcccca
     1021 tttgcggggt tgactgactc gatcgctctg ctatgcggct tttaatcttg ctctcttttt
     1081 tggtttcttt tcaacttcga ttgccggacg cgaatacatc tcaatcaact cttcatttgt
     1141 catgtctttt gtttcaagat acagactatc gcgaatcttg cgagtcatct cgacgcaatt
     1201 tattctaatc tttttcaact ttggtcacct cgcgagggga atatatctgg atctgcctgt
     1261 aacccatttc aaggttaact gagttgtaca atagaatctt gtcgaaacga acaatatgct
     1321 tgaaattcca gctaacaagt agatctgcat tcgcataggt cgcaattgca atatgcagaa
     1381 gatcgttggc atatttgggt ttcaaaattg cgtgcttctg atacgctgct cga
//
LOCUS       DAORHD010001412         1433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_125132_length_1433_cov_2.262700, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001412 DAORHD010000000
VERSION     DAORHD010001412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125132_length_1433_cov_2.262700"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..103)
                     /locus_tag="PKO06_22170"
     CDS             complement(<1..103)
                     /locus_tag="PKO06_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HNV72432.1"
                     /translation="MKTKKSAAKRFRVRAGGTIKRGQAFKRHILTKKT"
     gene            complement(277..798)
                     /gene="infC"
                     /locus_tag="PKO06_22175"
     CDS             complement(277..798)
                     /gene="infC"
                     /locus_tag="PKO06_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNV72433.1"
                     /translation="MATERSHRINGEITAPELRLIGVDNEPLGIVAFRDAIRMAEERE
                     VDLVEIAPTAAPPVCRLMDYGKFKYQEQKKAHEAKLKQKIIQVKEVKFRPGTDDGDYN
                     VKIRNLVRFLDEGDKAKITLRFRGREMAHQEFGVRLLERVRTDLDAVGQVEQIPRLEG
                     RQMVMIIAPKKKK"
     gene            complement(899..>1433)
                     /locus_tag="PKO06_22180"
     CDS             complement(899..>1433)
                     /locus_tag="PKO06_22180"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV72434.1"
                     /translation="TLKDALGRQWQCGTMQVDYNTAERLGAEYVAEDNGRRHPVMLHR
                     AIVGSLERFIGILIENHAGAMPMWLSPLQAVVMSITEAHADYAQSVVQALKKQGFRVQ
                     ADLRNEKINYKIREFSLQKVPYILVVGEKERQAGKVAVRARGGVDLGAIELDAFVERL
                     HQDISAKRSQPELPAKS"
BASE COUNT          231 a          453 c          415 g          334 t
ORIGIN      
        1 tggttttctt ggtgaggatg tggcgcttga acgcctgtcc ccgcttgatc gtgccgccag
       61 cgcggacacg gaaacgcttc gcagcgcttt tcttcgtctt catcttggca tggggccctc
      121 tgacttcctt tttgttgcct tgacaggtgt ccggcggatg ctgccggaac tttcgtgacc
      181 cgcttggctt ctggttgccg ggactttcgc cccggcaccg gccgcccggt gaagggcggc
      241 tcgtcttgcg acccatgcgc ctcgcggcgc gcgggtttac ttcttcttct tgggcgcaat
      301 gatcatgacc atctggcgcc cttccaaccg aggtatctgc tcgacctgtc cgaccgcatc
      361 gagatcggta cgaacccgct cgagcaggcg caccccgaac tcctggtgag ccatttcacg
      421 cccgcgaaac cgcagggtga tcttggcttt gtcaccttcg tcgaggaacc gcacgagatt
      481 gcggatcttg acgttgtagt cgccgtcatc ggttccgggt cggaacttga cttccttgac
      541 ctggatgatc ttctgcttga gcttggcttc gtgcgccttt ttctgttcct gatacttgaa
      601 cttgccatag tccatcaggc ggcagaccgg cggggcagcg gtgggcgcga tctcgacgag
      661 atcgacctca cgctcttcgg ccatgcgaat ggcgtccctg aaagcgacga taccgagtgg
      721 ctcgttatcg accccgatga gtcgcagctc gggcgcggtt atttcgccgt tgatgcgatg
      781 cgaacgttcg gtagcgattg tctcaacctc cagtcaaatg acattcaatg ctgcgcgtcg
      841 ggccaaccgg ccacccgtgg aaaggatgcc catcaccggc agaaccggtg acgcaccttc
      901 atgatttcgc gggcagttcc ggctggcttc gcttggcgga gatgtcctgg tgcagccgct
      961 cgacaaacgc atcgagctcg attgccccca gatccacacc gccgcgcgca cgcacagcca
     1021 ccttgcctgc ttgccgttct ttctcgccga cgacgagaat gtacggaact ttctgcaggc
     1081 taaactctcg gattttatag ttgattttct cgttacgcaa atcggcctgc accctaaacc
     1141 cttgtttttt cagcgcctgg acaacgctct gggcgtaatc ggcgtgggct tcggtgatgc
     1201 tcatgaccac cgcctgcagg ggcgacagcc acatcggcat ggcgccagcg tggttttcga
     1261 tcaggatgcc gatgaaacgc tcgagcgacc cgacgatggc gcgatgcagc atcaccggat
     1321 ggcgccggcc gttgtcctcg gccacgtatt ctgcacccag acgctcggcc gtgttgtagt
     1381 cgacctgcat cgtgccgcac tgccactggc ggcccagcgc atccttcagg gtg
//
LOCUS       DAORHD010001413         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_125465_length_1430_cov_4.191273, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001413 DAORHD010000000
VERSION     DAORHD010001413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125465_length_1430_cov_4.191273"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1430
                     /locus_tag="PKO06_22185"
     CDS             <1..>1430
                     /locus_tag="PKO06_22185"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HNV72435.1"
                     /translation="AATAFAEDGLAGVFASLPSTPEGDATRVRLLAGNEEAWYARWHL
                     IENAKESLTITYFIIDQDIFGYSFLGLLRKKARQGVTIRLMVDDRFLRGAKKLSDYDE
                     LQELAREPNVEIKVYNPIERQLLPALLDLRKVFISNHDKIIIADRRLCLTGGRNIGQD
                     YFVQLGEFKNVFQDLDVVMEGPGVVQQLQKAFDEEWKALPNRVVRRDPINLVSQSAKL
                     DLSYHLMNRYLQGMGVPDLQKLDFPRGLEKTARTLCAQLAGFKKLSSYASFRLFQGER
                     IAPVRILDRHSLLGTRRDIGPNLIRMIDAARDEILIQNAYVVLDEAAEAALRRASARG
                     VKIILCTNSGESTNHPSTVAFFLTDWKRLLATMPTARILVYPKGMPCIHTKAFVFDRQ
                     IAVVGTYNLDPLSMVINSEVVAVIHDTSFARRLALRMDADFAKCLEYKIRKEADGRIT
                     VVFGPDAHTEAEVMKKLERFGRLKFL"
BASE COUNT          284 a          437 c          400 g          309 t
ORIGIN      
        1 cggccgcgac ggcgtttgcc gaggacggtt tggccggggt ctttgcctca ctgccgtcga
       61 cccccgaggg cgatgcgacc cgcgtgcgtc tattggcagg caacgaagag gcttggtatg
      121 cgcgatggca cctgatcgag aacgcgaaag aatcgctgac catcacctac ttcatcatcg
      181 atcaggacat tttcggctac tcgtttctgg gccttctgcg caagaaggcg cgccagggcg
      241 tgacgattcg actgatggtc gacgaccgct ttctgcgcgg cgcgaagaag ctgagcgact
      301 acgatgaact gcaggaactc gcccgtgagc ccaatgtcga gatcaaggtc tataacccga
      361 tcgaacgcca gttactgccg gcgcttctcg atctgcgtaa ggtcttcatc tcgaatcacg
      421 acaagatcat cattgccgat cgtcgtctct gtctgacggg tggccgcaat atcggtcagg
      481 attatttcgt ccaactcggc gagttcaaga acgtgttcca ggatctcgat gtggtcatgg
      541 aaggccctgg cgtcgttcag cagctgcaga aggcgttcga cgaagagtgg aaagccctgc
      601 cgaatcgcgt ggttcggcgc gatccgatca atcttgtctc gcagtcggcc aagctcgatc
      661 tgtcctacca cctgatgaac cgatatctgc aggggatggg cgttcccgac ttgcagaaac
      721 tcgattttcc gcgcggcctc gaaaaaacgg cgcggactct ctgcgcccag ttggccggtt
      781 tcaaaaaact ctcttcgtat gcctcttttc gcctgttcca gggggaacgc atcgccccgg
      841 tgcgtattct cgaccgtcat tctctgctgg gcacccgtcg cgatatcggc cccaatctga
      901 tcaggatgat tgatgccgcc cgcgacgaga tactcattca gaacgcctac gtcgttcttg
      961 acgaggcggc cgaagccgcg ttgcgccgcg cgtccgcgcg tggggtgaag atcattctct
     1021 gcaccaacag cggagaatcg accaaccacc cgtcgacggt ggccttcttc ctcaccgact
     1081 ggaagcgact tctggcgacc atgcccaccg cccgtattct ggtctacccg aagggcatgc
     1141 cctgcattca taccaaagcc ttcgtcttcg atcgacagat tgccgtcgtc ggaacctaca
     1201 acctcgaccc gctcagcatg gtgatcaatt cagaggttgt cgcggttatt catgacacgt
     1261 cattcgcccg ccgcctcgcc ctgcgcatgg atgccgactt cgcaaagtgc cttgaataca
     1321 agattcgcaa ggaagcggac ggtcgcatca ccgtggtctt tggccccgat gcgcacaccg
     1381 aagccgaggt catgaagaag cttgaacgct tcggccgcct gaagtttctg
//
LOCUS       DAORHD010001414         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_125470_length_1430_cov_3.735273, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001414 DAORHD010000000
VERSION     DAORHD010001414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125470_length_1430_cov_3.735273"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PKO06_22190"
     CDS             <1..1008
                     /locus_tag="PKO06_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72436.1"
                     /translation="VHQKYYLWARVWSQIGSLKTTESYLDKALAVKADYPAALHLKGL
                     VHISRRHEDPSRITDGLKSLSAAREYEPRNPRILLSLSLAHEFSHQPAESIRFASQAV
                     SLSPDDPLLWSNLADLLQRHHALNEAAQAYRKALALIPSDPRLLNNLAFTLLELGQES
                     GAALTLARESVRLLPNAAFNLDTLAWAYHKNGMHAEALETIREARTLASGSLEIDFHF
                     GVIAMACGVLANPISFFTELGSRPELALDTQLQHRLVDVLASLTSTIASPSTGPFPKS
                     ETRPATETPRLTHGPASSATSASPLEPIAASSTSASATVAAPSDIPSTAPQSLPESSS
                     P"
     gene            1005..>1430
                     /locus_tag="PKO06_22195"
     CDS             1005..>1430
                     /locus_tag="PKO06_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72437.1"
                     /translation="MNVTRLLPRLLLVFTCLWLSSVTASEAVLSIDTSYDGQYRGQPA
                     FEKILADLPYVYQEALQKINRSLGISIPDKTQIIVVFSDHLTHNGLRLRGKRRSVEGP
                     RGILHYIYLDLEFLMTGQATLLEEMTHELTHAIMSETMGL"
BASE COUNT          303 a          456 c          360 g          311 t
ORIGIN      
        1 gttcatcaga agtactatct ctgggcccgc gtctggagcc agatcggctc actcaagacc
       61 accgagagct accttgacaa agcgcttgcc gtgaaagcag attatccggc cgctcttcac
      121 ctgaaaggac tcgtacacat cagcagacgt catgaagatc cttctcgaat caccgatgga
      181 ctgaaaagcc tgagcgctgc acgcgagtat gagccacgga atccgagaat tcttctttcg
      241 ttgagtctgg cgcatgagtt ctcgcatcag cccgcagaat ccatacgatt cgcctctcag
      301 gcggtatcgc tcagtccgga tgatccgctg ttatggtcga atctggccga tctgctgcag
      361 cgacaccacg cattgaatga agcggcccag gcctatcgca aggcactcgc gctcatccct
      421 tctgacccga gactgttgaa caatctcgca ttcactcttc tcgaactcgg acaggagtca
      481 ggagccgcgc tgaccctggc tcgcgaatcg gtacgactgt tacccaacgc cgccttcaac
      541 ctcgacactc tggcatgggc gtatcacaag aacggaatgc acgctgaggc gctcgagacg
      601 atccgtgaag cacgcaccct ggcatcggga tcgctggaaa tcgactttca cttcggggtc
      661 atcgccatgg cttgcggtgt gctcgcgaac ccgatttcct tctttacgga gttgggctct
      721 cgaccagaac tggcactcga tacccaactg cagcaccgcc tggtcgatgt tctcgcttct
      781 ctcaccagca ccatcgcatc gccttcgacc ggcccgtttc cgaagtcgga gacgcgacct
      841 gccaccgaaa caccgaggct cactcacggc ccagcatcgt cagcaacgtc tgcgtcaccc
      901 ctcgaaccaa ttgccgcatc ttcaacttct gcctcggcga ctgttgccgc gccctccgat
      961 atcccgtcaa ccgcgcctca atctcttcct gagagcagct ccccatgaat gtgactcgac
     1021 tgttgccacg tcttttgttg gtgttcacct gtctgtggct gtcctctgtc accgccagcg
     1081 aagcggttct cagcatcgac accagttacg atggacagta tcgaggccaa ccggccttcg
     1141 aaaagattct ggcggatctg ccgtacgtgt atcaggaagc gcttcagaag atcaaccgct
     1201 ccctgggaat ctcgattcca gacaagacgc agatcatcgt agtgttctcc gatcatctga
     1261 cacacaacgg tcttcgcctg cgtggcaaac ggcgctcggt tgaagggccg cggggtattc
     1321 tgcattacat ttacctcgac ctggagtttc tcatgaccgg tcaggcgacg ttgctcgagg
     1381 aaatgacgca tgaactgacc cacgccatca tgtcggagac catgggactg
//
LOCUS       DAORHD010001415         1429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_125663_length_1429_cov_2.904658, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001415 DAORHD010000000
VERSION     DAORHD010001415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125663_length_1429_cov_2.904658"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1429
                     /locus_tag="PKO06_22200"
     CDS             <1..>1429
                     /locus_tag="PKO06_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV72438.1"
                     /translation="YLSSVPELSAQNAPAGAVSAPVELVLNDTSLGSALQIMAEQAGK
                     KFEAHVPLEDKVNLDLRGLSFEDAVARVMAGKPYMYALDNETLHVFKGTGQPAPVADV
                     PVVQAPIQPQVQMAPPVVEKPKADRIFILKNRKAEELVVILKQLASEVGLVPDVRTNT
                     ILIKGFEEDVDRVASLAAQLDGMEPVRELEDKREYLSEVFSLEYVNDFADLENNLNMI
                     LYGQRGQGQGGQTTGLNQQMTTGGINQQYQEQMGETKPIPVRKEYYLLDKVRRVLMIT
                     AARDKMEIIREYFTRVNTPIAQVQIEAHIVAIDAGFERSLGINWNFQGGYTGPARPTA
                     NPLGIPQSTQQAGNNTNVDPTQGFEFGMWNLSNLSFVLQSAESQNKGRILSQPRVMTL
                     SGNEASIQIGTKYPYKQSTTQSNVGTTENVNYVDVGIILNVTPQVNSKARSIVMKLHP
                     EVSDVLGFRNDAPIITTRQTDTTVEV"
BASE COUNT          329 a          428 c          415 g          257 t
ORIGIN      
        1 gtatctgtcg tccgtgcccg aattgagtgc gcaaaatgcc ccggctggag cggtttcggc
       61 tccggtcgaa ctggtactca acgatacctc gctcggctcg gcgctgcaga tcatggcgga
      121 gcaggccggc aagaagttcg aggcgcacgt cccgctcgag gacaaggtca acctcgacct
      181 gcgcggcctc agcttcgaag acgccgtcgc ccgcgtcatg gccggcaaac cctacatgta
      241 cgctctcgac aacgaaacgc tgcacgtgtt caagggcacc ggacaacccg cgcctgtggc
      301 tgatgtcccg gtagtgcagg ctccgattca gcctcaggtg cagatggcgc cgccggtcgt
      361 cgagaagccc aaagccgacc gcatcttcat attgaagaac cgcaaggctg aagagctggt
      421 ggtgattctg aagcagctgg ccagcgaagt cgggttggtt cccgatgtgc gcaccaatac
      481 gattctcatc aaagggttcg aagaagacgt cgaccgcgtt gccagtctgg cagcccagct
      541 tgacggcatg gaaccggtac gcgagctcga agacaagcgc gagtatctca gcgaggtctt
      601 cagccttgag tatgtcaacg acttcgccga cctcgagaac aatctgaaca tgatcctcta
      661 cggacagcgc ggtcagggcc agggtggaca gacgaccggg ttgaatcagc agatgaccac
      721 gggtggaatc aaccagcagt atcaggagca gatgggcgaa acgaagccga ttcccgttcg
      781 caaagagtac tacctgctcg acaaggttcg ccgtgttttg atgatcaccg ccgcccgcga
      841 caagatggaa atcatccgcg agtatttcac ccgcgtgaac acgccgatcg ctcaggttca
      901 gattgaagca cacatcgtcg ccatcgacgc cggcttcgag cgctcactcg gcatcaactg
      961 gaacttccaa ggtgggtata cggggccggc tcgtccaacc gccaacccgc tgggtattcc
     1021 gcagtcgaca cagcaggccg gcaacaacac gaatgttgac ccgacccagg gcttcgaatt
     1081 tggcatgtgg aacctcagca atctgagctt tgtgctgcag tcggccgagt cgcagaacaa
     1141 gggccgcatc ctttcccagc cgcgcgtgat gactctgagc ggcaacgaag cctcgatcca
     1201 gatcggcacg aagtatccgt acaagcagtc gacgacacag agcaatgtcg gaaccaccga
     1261 gaacgtgaac tatgtcgacg tcggtatcat tctcaacgtt acgccgcagg tgaactcgaa
     1321 ggcgcgatcg atcgtcatga agctgcaccc cgaggtctct gacgtgctcg ggttccgtaa
     1381 cgatgcgcct attatcacga cccgacagac tgatacgacg gtcgaagtg
//
LOCUS       DAORHD010001416         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_126247_length_1425_cov_2.978102, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001416 DAORHD010000000
VERSION     DAORHD010001416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126247_length_1425_cov_2.978102"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1425
                     /locus_tag="PKO06_22205"
     CDS             <1..>1425
                     /locus_tag="PKO06_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNV72439.1"
                     /translation="LPDLAPLPEENSAVSSLETEIEDPNAPVSLDHHPIDKDLKDLAP
                     SIETEDLEPPRKKIELPPLDLLIVPPAREKAFDSRLNERKERILRTLNEFGIQAEIMD
                     VVEGPVVSRFELKPGPAVKAAKITSLETNLAMNLEATSIRIEAPIYGKNAIGIEIPNP
                     HPQPVFFHDLVKNDCFKNDDTCLNLALGVTIDARPVFADLSDMPHLLIAGSTGSGKSV
                     CVNTIIASILYQATAEQVKMVMIDPKMVELSSYNGIPHLISPVVTDPKKASAALLWAV
                     EEMTRRYSVMAKCEVKKISIYNQELERLRRDVDPMLEPMPFLVIIIDELADLMMTASA
                     EVEGSICRLAQMARAVGMHLVIATQRPSVDVLTGIIKANLPSRIAFAVTSQIDSRTIL
                     DTKGAESLLGKGDMLFVPKGKKPIRVQGAYISDRELQNIIAWVKQQGAPNYIDIAPTQ
                     EDDDEVEDPAPEEAGGDEDTRLLST"
BASE COUNT          341 a          417 c          377 g          290 t
ORIGIN      
        1 ttgcctgatc tggcccctct cccagaagag aattctgcag tcagtagcct ggagaccgaa
       61 atcgaggacc caaacgctcc tgtatctctc gaccatcatc ccatcgacaa agacctgaaa
      121 gatctcgctc cgagcatcga aaccgaagat ctcgaacctc cgcgcaagaa gatcgaactg
      181 ccacctctcg atctgctcat cgtgcccccc gcgcgcgaaa aggcgtttga ttcacggctg
      241 aatgagcgca aagagcgcat tctccgcacc ctgaacgagt ttggcattca ggccgagatc
      301 atggatgtgg tcgaaggtcc ggtcgtctca cgttttgaac tcaagcccgg cccggcagtc
      361 aaagcggcga agatcacttc tctggaaaca aatctggcca tgaatcttga agcgacttcg
      421 attcgtattg aagcaccgat ttatggaaag aatgccatcg gcatcgagat tccgaatccg
      481 catccgcaac cggtattctt tcacgatctg gtgaagaatg actgtttcaa gaatgacgac
      541 acctgcctga atctggcgtt gggtgtcacg attgatgccc gccccgtttt tgcagatctg
      601 agcgacatgc cacacctgct gatcgccggt tcaaccgggt caggcaaatc ggtctgcgtg
      661 aacacgatca tcgccagcat tttgtatcaa gccacggcgg agcaggtaaa aatggtgatg
      721 atcgacccga agatggtcga actctcgagt tacaacggga tcccccacct gatttctccg
      781 gtggtcactg atccgaaaaa ggcttcagca gcattgttgt gggcggtcga agagatgacc
      841 cggcgctaca gcgtcatggc caagtgcgag gtcaagaaga tctcgatcta caatcaggag
      901 ctcgagcgcc tgcggcggga cgtcgatccc atgctcgagc caatgccttt cctggtgatc
      961 atcatcgatg agctcgccga tctgatgatg accgcctcgg ctgaggttga aggctcgatc
     1021 tgtcgactgg ctcaaatggc ccgtgccgtc ggcatgcacc tggtcatcgc cacccaacgc
     1081 ccgagcgttg acgttctgac cggtatcatc aaggccaatc tgccctcgcg catcgcgttc
     1141 gcggtcacct cacagatcga ttcccgaacc attctcgata ccaaaggcgc agaatcgctt
     1201 ctcggcaagg gggacatgct gttcgtcccc aaaggcaaaa agccgattcg cgtacagggt
     1261 gcctacatca gcgatcgcga actgcaaaac atcatcgcgt gggtcaaaca gcagggggca
     1321 ccgaattaca tcgacatcgc tccgacgcag gaagatgacg acgaggtgga agatcccgct
     1381 cctgaagagg cgggaggcga tgaagacacc cgtcttctct ccacg
//
LOCUS       DAORHD010001417         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_126671_length_1422_cov_3.464521, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001417 DAORHD010000000
VERSION     DAORHD010001417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126671_length_1422_cov_3.464521"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PKO06_22210"
     CDS             complement(<1..942)
                     /locus_tag="PKO06_22210"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV72440.1"
                     /translation="MASSQLFQARDHGNGVLEIIAVFPDMNAEHPAMQAVVTLARRFP
                     TLILTLESGASIDASGFGKLIEASGMTRLFVATSDQQAHPFARASIRTYATPREALLA
                     VRGEEIARHIVAKMEAIPELHSQAFDLLQKLNQPSLSFEKMGETIAKEPGLATQILKT
                     ANSAFFMRRSKVDTIPAALTLLGLEGLKQILVVNVFKGLTGYFGAQKEVLDHGRVCAQ
                     LAVHLAELKKVPRTNLAKIRLGGLLHDVGSLALVFFYPKEYGEVRALMKTQTKRSYEA
                     ELAVFGIEHQALGKQLAEKWGFPDYLCRIIGDHHAMSD"
     gene            1191..>1422
                     /locus_tag="PKO06_22215"
     CDS             1191..>1422
                     /locus_tag="PKO06_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72441.1"
                     /translation="MRDDAGFKMEPTPVSSGNPFLARYQVSLRTRLPCYEALAMRQKE
                     NHRFYEGSIFPHTAQAGTPFSEPATPAVAGSVS"
BASE COUNT          301 a          433 c          375 g          313 t
ORIGIN      
        1 gtcgctcatc gcatggtgat cgccgatgat gcggcagaga tagtcgggaa acccccactt
       61 ttccgccagc tgctttccca gcgcctgatg ttcgatgcca aagaccgcca gttccgcctc
      121 gtaactgcgt ttcgtctgcg tcttcatcag cgcgcgcact tcgccatact ccttcgggta
      181 aaaaaagacc agggccagac tgccgacatc gtgcagcaac ccgcccagcc tgattttcgc
      241 cagattggtt ctgggcacct tcttcaattc ggcgagatga accgccagtt gagcacacac
      301 tcggccatga tcgagcactt ccttctgcgc cccgaaatac ccggtcaacc ccttgaacac
      361 gttgacgacc agaatctgct tcagtccttc aaggccaagc agggtcagag ccgccggaat
      421 cgtgtcgact ttgctgcgac gcatgaaaaa cgccgaattc gcggtcttca gaatctgcgt
      481 ggccagcccg ggctccttgg cgatcgtttc gcccatcttc tcgaagctca ggctcggctg
      541 attcagcttc tgcagcaggt caaacgcctg cgaatgcagt tccggaatgg cttccatctt
      601 cgccacgata tggcgcgcga tctcttcacc gcgaaccgca agcagagcct cacgcggcgt
      661 ggcatacgta cgaatcgaag cccgagcaaa ggggtgtgcc tgctggtcag aggtggcaac
      721 gaacaaacgc gtcattcccg aagcctcgat cagctttccg aacccggagg catcaatcga
      781 tgctcccgat tcaagcgtca gaatcaacgt gggaaatcgg cgcgccagcg tcacgaccgc
      841 ctgcatcgcc gggtgttccg cgttcatatc gggaaacacg gcgatgatct cgagcacccc
      901 gttgccgtgg tcgcgagctt gaaagagttg tgaagaggcc atgtctgatc ctttgagctc
      961 gaggtaccga ccggcgaacg cccaggccgg caccgccgac agagtgagaa cgaaatcata
     1021 ccatgatcga cgagcgcacg acgaatcccg ggctcgacga cgcttgagat tgccctgttc
     1081 atcgcatttt taccgtctga cggtcaaacc cggtgaattc gctcttgatc cgaagggaat
     1141 cgtgtgatat ctttggttca gtggctcttt gaaaatcatg tccctgcggt gtgcgtgatg
     1201 atgcaggttt taaaatggag ccaacacctg tatcttccgg gaatcctttt ctcgcccgtt
     1261 atcaggtctc cttacgaaca aggcttcctt gttacgaagc cctggcaatg aggcagaagg
     1321 aaaaccatcg tttttacgaa ggttccattt ttcctcacac ggcacaggcg gggacaccct
     1381 tttctgaacc ggccacccct gcggtggccg gttctgtttc tc
//
LOCUS       DAORHD010001418         1422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_126716_length_1422_cov_2.566203, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001418 DAORHD010000000
VERSION     DAORHD010001418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126716_length_1422_cov_2.566203"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PKO06_22220"
     CDS             <1..374
                     /locus_tag="PKO06_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72442.1"
                     /translation="GIPTRSNAGARRPMRFEDLFAIQMIVDEQRPQFEKQARELFDQL
                     TPVQRFFAWAQYHDWRHTKIAEHLNVGLSRVSNLQHQYILRFRKAIDPTWTHEELRAF
                     FEVWYCLLHHEPRPTLPEARS"
     gene            371..1207
                     /locus_tag="PKO06_22225"
     CDS             371..1207
                     /locus_tag="PKO06_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72443.1"
                     /translation="MKHPTPGAIAPLPDESRRSTPFAVMSLIMRTDSHSVLLAPVHAE
                     IELASEDDLVCAAAGSSLKVDLVICMGLARSFPLSAAGESTTSLAPDLLAILLNTSAQ
                     PGRILERRLVRGLHAPPGSPAGKAKRALSEFWQTFRGSQWEKALLALANRQGSASSRE
                     TLLDIAHLVPTLSRVSSAPAAAPAQRKTATASPMAGKGSALRTPDRPPRKSKDAHRTP
                     TGKPKPPASPTHTSAGESPIHRFLEMLRRLGVQVDRIQGSDAGTDYLSLLSDLFALIE
                     RP"
     gene            complement(1214..>1422)
                     /locus_tag="PKO06_22230"
     CDS             complement(1214..>1422)
                     /locus_tag="PKO06_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72444.1"
                     /translation="HDENPIEDDGWYTCTWADLANNRAAAVGDRVFVGVMTPDRRRFL
                     GYRYVTLTEENLRNGYVLVNIVIK"
BASE COUNT          280 a          465 c          376 g          301 t
ORIGIN      
        1 gaggcatacc gactcgatcg aacgcgggtg cgcggcgacc catgcgattc gaagaccttt
       61 tcgccattca gatgatcgtc gacgagcaaa gaccgcagtt cgagaaacag gcgcgagagc
      121 tgttcgacca gctcaccccg gttcagcggt tctttgcctg ggcccagtat cacgactggc
      181 gccacaccaa aatcgcggag catctgaacg ttggtctgtc gcgcgtttcg aaccttcagc
      241 accagtacat tctgcgattc agaaaagcca tcgaccccac ctggacacat gaagagcttc
      301 gggccttctt cgaagtctgg tattgccttc tgcatcacga accccgcccc acccttccgg
      361 aggcccgctc atgaaacacc ctaccccggg cgcgatcgcg ccgctccctg acgagtctcg
      421 acgttcgact cccttcgctg tcatgtctct gatcatgaga acagattcac attcggttct
      481 gttggcgccg gtacacgcgg agatcgaact ggcttctgaa gacgatctcg tctgtgccgc
      541 cgcaggcagc tccttgaagg tcgatctcgt catctgcatg ggcctcgccc gatcgtttcc
      601 gctttcagcc gcaggcgaat cgactacctc gctcgccccc gatctgctcg caattctctt
      661 gaacacatcc gcgcaacccg gtcgaattct tgaacgcaga ctggtgcgcg gattgcacgc
      721 tcctcctggt tcacccgccg ggaaagcgaa acgagcattg tccgagttct ggcagacgtt
      781 tcgcggctcc caatgggaga aagcgttact ggctctggca aatcgccagg gttctgcttc
      841 gtcacgcgag acactgttgg atatcgcgca tctggtgcct accctttccc gcgtgtccag
      901 cgcgcctgcc gcggcaccag cccagcgcaa aacggcgacg gcatcaccca tggccggcaa
      961 agggtcggcg ttgcgcactc ccgatcgtcc gcctcgaaaa tcaaaagacg ctcatcgaac
     1021 gccgactggc aagcccaagc caccagcgtc accgactcat acttccgctg gcgagtcgcc
     1081 gatccatcga ttcctcgaga tgctccgtcg acttggagta caagttgatc gtattcaagg
     1141 ttcagatgca ggaacagatt acctgagcct gctctctgat ttgttcgccc tcatcgagcg
     1201 accgtaaacg agttcatttg atgacgatgt tgaccagcac atatccgttg cgtagattct
     1261 cttccgtcag cgtcacgtac ctgtagccga gaaaccttcg tcgatcaggc gtcatcactc
     1321 cgacgaagac gcggtcgccc actgcggcgg cgcggttatt ggccaggtcg gcccaggtgc
     1381 aggtgtacca gccgtcgtcc tcgatcgggt tctcgtcgtg cg
//
LOCUS       DAORHD010001419         1421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_126845_length_1421_cov_3.199854, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001419 DAORHD010000000
VERSION     DAORHD010001419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126845_length_1421_cov_3.199854"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1421)
                     /locus_tag="PKO06_22235"
     CDS             complement(<1..>1421)
                     /locus_tag="PKO06_22235"
                     /inference="COORDINATES: protein motif:HMM:NF025181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tubulin-like doman-containing protein"
                     /protein_id="HNV72445.1"
                     /translation="FMVNYGPIGKSHDTTTFGAISEETPFDQCYLLGNPELMDIPTYY
                     SIAGELVFTKLATNLRQAQNSMLDNASQVLGQLSSDLEGKQLKAFSSAGYKSFYYPLD
                     TIHQLYTYKLSMDLTYWLKQATDKITVDGMVTEFVESNPEKLDLSPEGLFTLAKGIIQ
                     RKQYWDNPQYHTMRTDPAQQPGESAAAYYARKIDEMERVKVKKDEIRARVKVDFERAA
                     IEAGKYLRTKVIEVINNPARGAQMAIDFLVGFDKHLARFPREVLVQKDNELKGKQAKE
                     QREYQQARDALKSRLESMFGLGGKLLEWGSILKVANIHEEFNKLEKEANEAVDIEYER
                     FCFERAGDFLKFLQVHAQNLRKQVEEFVRKLELISKNGYIERSYEQCLERIKPYTREQ
                     AFVRTYIFEERDIEPFYKYLIGDLTNDDIEKNFLVGIELDKNWDAYTNPTSDSLENDI
                     VKYCSGTISKRLFGGIEEFIHWK"
BASE COUNT          272 a          368 c          395 g          386 t
ORIGIN      
        1 ccttccagtg gatgaattct tcgataccac cgaagagtcg cttgctgatt gtgcctgaac
       61 aatacttgac gatgtcgttt tccagcgaat cactggtcgg attggtgtac gcgtcccagt
      121 tcttgtcgag ctcgatgccg accaggaagt tcttttcgat atcatcattt gtcaggtcgc
      181 cgatcagata cttgtagaac ggctcaatgt cgcgctcttc gaaaatatag gttcggacaa
      241 atgcctgctc gcgcgtatat ggcttgatgc gttcgaggca ctgttcgtaa ctgcgttcga
      301 tgtacccgtt cttcgagatc agttcgagct tgcgcacgaa ttcttcgacc tgtttgcgca
      361 ggttctgggc atgcacctgc aggaacttga gaaaatcgcc ggcgcgttcg aaacagaacc
      421 gctcatactc aatgtcgacg gcttcgttgg cttccttctc gagcttgttg aactcttcgt
      481 gaatattggc gactttgaga atcgaacccc attcgagcag tttgccgccg aggccgaaca
      541 tcgattcgag acgggatttc agcgcgtcgc gggcctgctg atattcgcgt tgttccttgg
      601 cttgtttgcc tttcagctcg ttgtctttct gtaccagcac ttcgcgtggg aaacgcgcca
      661 gatgcttgtc gaaaccgaca aggaagtcga tcgccatctg tgcgccacgg gcgggattgt
      721 tgatgacctc gatgactttt gtgcgtaagt acttgccggc ttcgatggcg gctcgctcaa
      781 aatctacctt gacgcgggcg cgaatctcgt ctttcttcac cttgacccgc tccatctcat
      841 cgatcttgcg cgcgtaatag gcggcggcag attcgcccgg ctgttgcgcc ggatcagtgc
      901 gcatcgtgtg atactgcgga ttatcccaat actgtttgcg ttggatgatg cccttggcga
      961 gggtgaaaag cccttcgggg ctcagatcga gcttttcggg attcgactcg acgaactcgg
     1021 tgaccatgcc gtcgacggtg attttgtccg tcgcctgctt cagccagtag gtgagatcca
     1081 tcgacagttt gtaggtgtac aactggtgaa tcgtgtcgag cgggtagtag aagcttttgt
     1141 aaccggctga actgaaagcc ttgagctgct tgccctcaag gtctgagctc aattgcccca
     1201 ggacctgcga cgcgttgtcc aacatcgagt tctgcgcttg acgcagattg gtggccagtt
     1261 tggtgaagac cagttcgccg gcgatgctgt aatacgtggg gatatccatc aattcagggt
     1321 tgccgagcag atagcactga tcaaatggtg tctcttcgct gatcgcgccg aatgtcgtcg
     1381 tgtcgtgact tttgccgatc gggccgtagt tgaccatgaa c
//
LOCUS       DAORHD010001420         1418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127278_length_1418_cov_3.554659, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001420 DAORHD010000000
VERSION     DAORHD010001420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127278_length_1418_cov_3.554659"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PKO06_22240"
     CDS             <1..649
                     /locus_tag="PKO06_22240"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV72446.1"
                     /translation="EVALVNLAAADLGGDYCDVVRTRNGQVLFVIGDVTGHGVSSALL
                     MAMAKAMVFRFAEEGGELGRFLERLNRMVFRVLHRKKMMTFFACLLDPAEGVLTYSTA
                     GHPYPIVVRANGTLERLRLIHSPLGFRDPCEPFVVQTDRLLPGDLLFLYTDGILEARN
                     ADGEYFGKQRLEEILRRTSSVNPAAVRESVVQSVQAFQQMADDDLTLLAVKRSSE"
     gene            840..>1418
                     /locus_tag="PKO06_22245"
     CDS             840..>1418
                     /locus_tag="PKO06_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72447.1"
                     /translation="MLKKLIVTGLICCATTTAFAHDIFDIFGRGPSFRHGKGTSSPVQ
                     SAPVQQSGQSSQAGQSWQTGQYQPVGQTGWNNHSGHNGQYSGGYAIDNVSVALLELVA
                     NDCEMCISSLRHGAWANNVMSIGYVNNALSALRRSRVHQSYRRLVNELEDRLQRVRFY
                     LLMNDTGDARARLNQISSMVRRVVASIRNGGAN"
BASE COUNT          258 a          375 c          454 g          331 t
ORIGIN      
        1 tgaggtcgcg ctggtgaatc tggccgccgc tgatctgggt ggtgattact gcgatgtggt
       61 gcgtacacga aacggacagg tgttgttcgt cattggtgat gtcaccgggc atggagtcag
      121 ctcggccctg ttgatggcca tggccaaggc gatggtcttt cgttttgccg aggaaggcgg
      181 ggagctgggg cgctttctcg agcgactgaa ccgcatggtg tttcgggtgc tgcatcggaa
      241 aaagatgatg accttcttcg cctgtctgct cgacccggcg gagggggtgc tgacctactc
      301 gacggccgga cacccgtatc cgatcgtggt gcgtgccaat ggcaccctgg aacgcctgcg
      361 gttgattcat tctccgttgg ggttccgcga tccctgcgag ccgtttgtgg tgcagacaga
      421 tcgtttgctg ccgggtgatc tgttgtttct ctataccgat gggattctgg aagcccggaa
      481 cgctgacggc gaatatttcg gaaagcagcg gctggaagag attctgcggc gcaccagctc
      541 ggtgaacccg gcggcggtgc gcgagtccgt tgttcagagt gtgcaggcct ttcagcagat
      601 ggcggatgac gatctgacgc tgctggcggt gaagcgctcg tccgagtagg ggtgcgggaa
      661 actcggcagc ggagtgcccc attttcagca gggccgcgga gagcgttgca tactttttgc
      721 atcatcctcc ctgtcgcgcg cttcctgaca caagattccc gctgtgatcg agtgagccga
      781 aagtctggca tcaatattgc tttacagtcc tcgcatttca ttacgttgga ggacatgcca
      841 tgttgaagaa actcatcgtg accgggctca tttgttgtgc gacgaccacg gcttttgccc
      901 atgacatttt tgatatcttc ggtcgcggac cgagcttccg acacggaaaa ggaacctcgt
      961 caccggtaca atcagcgccg gtgcagcagt cgggtcagtc gtctcaggcg ggtcagtcat
     1021 ggcagaccgg gcagtatcag ccggtcggtc agacgggttg gaataaccac agcggccata
     1081 acggtcagta ttccggcgga tatgcgatcg acaatgtcag cgtcgcgctg ctcgaactgg
     1141 tcgcgaatga ttgcgagatg tgcatcagtt cgctgcgcca cggagcctgg gccaacaacg
     1201 tgatgtcgat cggctatgtg aacaatgctt tgtcggccct gcgccgcagc cgggttcatc
     1261 agtcctatcg ccgcctggtg aacgaacttg aagatcgtct gcagcgtgtg cgcttctatc
     1321 tgctgatgaa tgacacgggt gatgcgcgcg cccgtctcaa tcagatttct tccatggttc
     1381 gccgggtcgt ggcttcgatt cgcaacggcg gagcaaac
//
LOCUS       DAORHD010001421         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127490_length_1417_cov_2.615272, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001421 DAORHD010000000
VERSION     DAORHD010001421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127490_length_1417_cov_2.615272"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKO06_22250"
     CDS             complement(<1..347)
                     /locus_tag="PKO06_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72448.1"
                     /translation="MKRVVWMLLLAAVLMVLPVQAEDDEGYAAVAYPAFDTLTNEIEI
                     LGTFVYTDQQSPDIELKALFRPVMIDVNMKDGQWKTMNANMASRYTPEVTIQNPGTDC
                     SFKLTMDFAKSKE"
     gene            complement(433..>1417)
                     /locus_tag="PKO06_22255"
     CDS             complement(433..>1417)
                     /locus_tag="PKO06_22255"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV72449.1"
                     /translation="APPSSTRPVIRPAGSTPPPSMPLEDPFSNPLPQSKATTPPSVTR
                     TAPAPLPVQTSTGGLPSRYQQITLIGSGGMGMVYKAFDANLQRWVAIKTFREEAASRH
                     PEVLKRFLNESRIMASLDHPTIPKVFDLSVASPYYLSFEFLEGDDLRKVIGSFSTEAP
                     PDLQGCLTLGIQLASGFHHAAEKGILHRDIKPENLILETGGRVRIIDFGLAHLEGSMN
                     MTESGVVIGTPWYLAPERLQGQPASVATELYAFGFALYELLTTVRPYPGDAVTVVFAR
                     DPIPLRQMRPDAPPELESVLARCLSKNPLTRPTNFAEIESLLTGILHQYYG"
BASE COUNT          315 a          409 c          396 g          297 t
ORIGIN      
        1 ttttccttcg acttcgcgaa gtccatcgtc agcttgaatg agcagtccgt tccggggttc
       61 tgaatcgtga cttcgggggt ataacgactg gccatgttgg cattcatcgt cttccactgg
      121 ccatccttca tattgacatc gatcatcacc gggcgaaaca gcgctttcaa ttcaatatcg
      181 ggagactgct gatcggtata gacaaaggtt cccagaatct cgatctcgtt cgtcaaggtg
      241 tcaaaggcag gatacgcgac tgcggcatac ccttcatcgt cttcggcctg caccgggagc
      301 accatcaaca cggctgccaa caagagcatc cacacgactc gtttcatacg cacctcctgt
      361 atcgttcgat ttctggaaac tcaccatctt cccttcatgg tatcacaagc actctctcgg
      421 ggccaaaaac agttaaccgt agtattgatg caggattccg gtcagaagcg actcgatttc
      481 cgcaaagttc gtcgggcgcg tcagagggtt cttgctcaaa caacgggcca gaacagattc
      541 caattccggc ggcgcatccg gccgcatctg cctgagcgga atcggatcgc gcgcaaagac
      601 caccgtcacg gcatcgccag gatacgggcg aaccgtcgtc agcaactcgt acaacgcgaa
      661 accgaacgca tacagttccg tcgcgacaga ggcgggttgc ccctgcaatc gttccggtgc
      721 cagataccac ggagtcccga tcaccacacc agattccgtc atgttcatcg aaccttccag
      781 gtgtgccaga ccgaagtcga tgatccgaac tcgtcctccc gtttccagaa tcaggttctc
      841 gggtttgata tcgcgatgca gaatgccctt ttcagcggcg tgatgaaagc cactcgccaa
      901 ctgaattccc agggtcagac acccctggag atccgggggc gcttcggtgc tgaagctgcc
      961 gatgaccttg cggagatcat caccttcgag aaactcgaat gacaggtaat agggagacgc
     1021 caccgagaga tcgaacacct tgggaatcgt cgggtgatcg agcgaggcca tgatgcgcga
     1081 ctcgttgaga aaacgtttca gtacttccgg atggcggctc gctgcttctt cgcgaaaggt
     1141 cttgatcgcc acccaacgct gcaggttggc atcgaaggcc ttgtagacca tgcccatgcc
     1201 gcccgaccca atcagggtga tctgctgata acgactcggc aggcccccgg tcgaggtctg
     1261 aaccggcaat ggagcgggtg ccgtacgcgt cacggaaggt ggcgttgtag ccttcgactg
     1321 gggcaatggg ttcgagaagg ggtcttccaa tggcatactg ggagggggag tcgagcccgc
     1381 gggacgtatc actggacgcg tggacgacgg gggggct
//
LOCUS       DAORHD010001422         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127507_length_1417_cov_2.442731, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001422 DAORHD010000000
VERSION     DAORHD010001422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127507_length_1417_cov_2.442731"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..981)
                     /locus_tag="PKO06_22260"
     CDS             complement(1..981)
                     /locus_tag="PKO06_22260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72450.1"
                     /translation="MMKSNRHALTLVELIVGLAIFCSIGAVFVTFITSSSKEVKFSGD
                     HFNAVILSQKVSEDVLEELSVNPYGLETLGVDGTKSGYQEVVDGKSVFFSYLEDRVAP
                     WGKIEPTTDGLLNSDMKPLYDHVKKYKFQVAGERMHNTGDDPKRNLMSCDIDFNWSSQ
                     TGRGEFNTSFLLFSPATARKTDLAAIVDQSAIDARIPGEVFFRPGKTVPELAADIGEN
                     VDTLLALGRISLVSRDFMASQYYQKRRQDIKLAKLQLSATPQIDLANQFELRKTIARM
                     WYDLAKTCFQVIAYLEPQFAVLQSQGKFGTGAGTGFSPITTSPYICTKRR"
     gene            complement(978..>1417)
                     /locus_tag="PKO06_22265"
     CDS             complement(978..>1417)
                     /locus_tag="PKO06_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72451.1"
                     /translation="FQSVNKTQKSFSQQVQLQMESRKAFDQVVDQIREGTDVIRPVIG
                     ETLPYLVFKDIVNQTTMLYLEPNDEMSKRLKQRVYRLISYRTDYSGSYKSTLERPLME
                     SIKRIRFSCLSPNSVQVNATIITDKGEYQFLAHVGLMNLGGFE"
BASE COUNT          310 a          364 c          409 g          334 t
ORIGIN      
        1 ttaccgccgt ttcgtacaga tgtacggcga cgtggtgatg ggactgaaac cagtacccgc
       61 gccggtgccg aactttccct ggctctgcag aacggcgaat tgaggttcaa gatacgcgat
      121 aacctgaaaa caggtcttgg ccaggtcata ccacatgcgg gcgatggttt ttcgcaattc
      181 gaactggttg gcgagatcga tctgcggggt tgctgatagt tgcagtttcg ccagtttaat
      241 atcctgccgg cgcttctgat aatactgtga ggccatgaag tcacgcgaga cgagtgagat
      301 acgccccaga gccagcagtg tgtccacgtt ctcgccgatg tccgccgcca gttcgggcac
      361 ggtctttccc ggtcgaaaga agacttcgcc gggaatgcga gcatcgatgg ccgactggtc
      421 gacgattgcc gccagatcgg ttttgcgtgc cgtggcgggc gaaaagagga gaaaactggt
      481 gttgaattct ccgcgcccgg tctggctgga ccagttgaaa tcgatgtcgc aggacatcag
      541 attgcgtttc ggatcatctc cggtattgtg catccgctcg ccggcaacct ggaatttgta
      601 cttcttgaca tgatcgtaga gcggcttcat gtcggagttg agaaggccat cggttgtcgg
      661 ttcgattttt ccccaggggg cgacacggtc ttccaggtac gagaaaaaca ccgacttgcc
      721 atcgacgact tcctgatacc cggacttggt tccgtccacg cccagggttt caagaccgta
      781 agggttgacc gataactcct cgaggacatc ttcgctgact ttttgtgaca gaatgacggc
      841 gttgaagtgg tcccccgaga acttcacctc tttcgaagat gaggtgatga aggtgacgaa
      901 gacggcgccg atggaacaga aaatggcaag accgacgatg agttcgacca gggtcagagc
      961 atgacgattc gatttcatca ttcaaacccc ccaagattca tgaggccgac atgagccaga
     1021 aactggtact cacccttgtc ggtgatgatg gtcgcattga cctggacgct gttcgggctc
     1081 aggcacgaaa aacgaatgcg tttgatcgat tccatcagcg gtcgctccag cgtcgacttg
     1141 tacgatccgg aatagtcggt gcgataggag atcaggcggt agacgcgctg cttcaacctt
     1201 ttcgacatct cgtcatttgg ttcgagatac agcatggtgg tctgattgac gatatccttg
     1261 aacacgagat agggcagggt ctcgccgatc accggtcgaa tcacatctgt tccttcgcgg
     1321 atctggtcga ccacctggtc aaaggctttc cgggattcca tctgcagctg cacctgctgc
     1381 gagaagcttt tctgggtctt gttcacggac tggaaga
//
LOCUS       DAORHD010001423         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127588_length_1416_cov_4.029390, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001423 DAORHD010000000
VERSION     DAORHD010001423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127588_length_1416_cov_4.029390"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKO06_22270"
     CDS             complement(<1..275)
                     /locus_tag="PKO06_22270"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HNV72452.1"
                     /translation="MQNCAESMKKAQETIAEQTVDALKKAAKLTSLSGQDLDVPVGQT
                     PHEVVYRKHRVKLLRYLPTGKTVAQTPILICFALVNRPYVMDLIPGK"
     gene            complement(279..746)
                     /locus_tag="PKO06_22275"
     CDS             complement(279..746)
                     /locus_tag="PKO06_22275"
                     /inference="COORDINATES: protein motif:HMM:NF019494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HNV72453.1"
                     /translation="MSEEKTEKKPILIKKYQNRRLYNTEESRHMTLEEIAKLIREGHD
                     IRVVDSHGNQDITKQILAQIILEEEKNQKDLLPVSLLYQIIRANEDLTRDFFENYLHS
                     MFDSYIAHRKLVEKRLTEMRDLSKLPFELSDLFIRSFGVAGQGGNKPKPDKEG"
     gene            complement(859..>1416)
                     /locus_tag="PKO06_22280"
     CDS             complement(859..>1416)
                     /locus_tag="PKO06_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV72454.1"
                     /translation="DVTNRAQIEAAVDEIMRVDGRIDVLVNNAGITADAMLHKMEEAQ
                     WDRVVNVNLKGVFNCSQIVSKVMRDQSSGSIINTSSVVGVYGNIGQTNYAATKAGLIG
                     MTKTMAKELGMKNIRVNAVAPGFIMTDMTAKMPEKVLDLMKEKAPLRRHGTVEDVANC
                     YLFLASNESSFVTGQVLGVDGGIVL"
BASE COUNT          286 a          406 c          334 g          390 t
ORIGIN      
        1 ctcttgccgg gaatcagatc catgacgtag ggacggttca ccagagcaaa gcagatcaga
       61 atcggtgtct gagccacggt tttgccggtc ggcagatagc gcagcagctt gacccggtgt
      121 ttacgataga caacttcatg cggcgtctgc ccaaccggca catcgaggtc ctgacccgac
      181 agcgatgtca gtttcgccgc cttcttgagc gcatccaccg tctgctcggc gatcgtttcc
      241 tgggctttct tcattgattc agcgcaattt tgcatggttc acccctcctt atcgggtttc
      301 ggtttgttcc ctccctggcc ggcgactcca aagctgcgaa tgaacaggtc gctcagctcg
      361 aagggaagct tgctcagatc tcgcatctcg gtcaggcgct tttcaaccag tttgcgatga
      421 gcgatatacg aatcaaacat ggaatgcagg taattctcga agaaatcgcg ggtcagatct
      481 tcgttggcgc ggataatctg atacaggaga ctcaccggga gcaggtcttt ctggtttttt
      541 tcttcttcga gaatgatctg cgccagaatc tgcttggtga tatcctgatt gccatgtgaa
      601 tctacaactc tgatatcatg accttctcga atgagttttg caatttcttc gagtgtcatg
      661 tggcgacttt cttcggtgtt atacaaccgt cggttctggt atttcttaat gaggatcggc
      721 ttcttttctg ttttttcttc tgacatattc atcacctttc agtaaaatgc gttcccaatt
      781 ccattctggt attttgcatt cgctcttcgc gttcgcgttt cgcctgagcg aagagcgctt
      841 cttacagccc atcgcacact atagaacaat ccctccgtca actccgagca cctgcccggt
      901 aacgaacgac gattcgttcg aagccagaaa cagataacag ttggcgacat cttccaccgt
      961 gccgtgacgt cgcagtggcg ccttctcctt catcagatcg agcaccttct caggcatctt
     1021 cgcggtcatg tcggtcatga tgaagccagg ggccacggcg ttcacccgaa tgtttttcat
     1081 ccccagttcc ttggccatgg tcttggtcat gccaatgagt ccggcttttg tcgcggcgta
     1141 gttcgtctgg ccaatgttgc catacacccc gaccacggaa ctcgtattga tgatcgagcc
     1201 actgctctga tcgcgcatca ccttcgagac gatctgcgag cagttgaaaa cccccttcag
     1261 gttgacgttt accacgcgat cccactgcgc ttcttccatt ttgtgcagca tggcgtcagc
     1321 cgtaataccc gcattgttca ccaacacatc gatacgaccg tcaacccgca tgatttcgtc
     1381 caccgccgct tcgatctgcg cgcgattcgt cacatc
//
LOCUS       DAORHD010001424         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127620_length_1416_cov_3.146951, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001424 DAORHD010000000
VERSION     DAORHD010001424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127620_length_1416_cov_3.146951"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1416)
                     /locus_tag="PKO06_22285"
     CDS             complement(<1..>1416)
                     /locus_tag="PKO06_22285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV72455.1"
                     /translation="GRAEGCTIKLTSIAISREHVLVRYSPDSGASIQDLHSTFGTHVN
                     GHRVQAGVFVPLVTGSRVQLAQDVFLTLERDIAIEADGDKGNETLAATSEPGTEVQVF
                     PFFLNRNVNLVRDTFAQLRDKIPPTARDDLQATETVISSRIRELSAILEVSFALGSIR
                     SFQRLLEYTIDMALQVTGAERGGLILFNENTQRFESAVVRRMGLQDVEKDMQTSESLI
                     QKCFLTGETLVIRDTSVDPLVAGNTSIVINKILSIAVTPLRIQNNVIGVLYLDNRLSA
                     NTFTERVQELLRVFATMASQAIYNSRLLHQATTDSLTGLYNKKHFLQRLSEECARARR
                     HRTDLSLLMLDIDHFKSFNDTHGHVIGDKVLLLVSRSLRDNVRVHDVCARYGGEEFAV
                     ILPQTPVEGAASLAEKLRKSIEQLPLRFGDKKLTITISVGVAGFDSLIMEKPATFVQY
                     ADESLYKAKHSGRNQVQVFSGS"
BASE COUNT          304 a          390 c          417 g          305 t
ORIGIN      
        1 ggaaccggaa aagacctgta cctggtttct tcccgagtgc ttggctttat agagcgattc
       61 gtcggcatac tgcacgaagg tggcgggttt ttccatgatc agcgaatcaa aacccgcgac
      121 tccgacactg atggtgatcg tcagcttctt gtcgccaaaa cgcaggggca gttgttcgat
      181 cgacttgcgc aacttctcgg ccaggctcgc ggcgccctcg acgggggtct ggggaagaat
      241 gacggcgaac tcttctccgc cgtaccgagc gcaaacatcg tgaactcgca cgttatcgcg
      301 cagactgcga ctgaccagta gcagcacctt gtcgccaatg acatgcccgt gcgtgtcatt
      361 gaacgacttg aaatgatcga tatccagcat cagcaggctc aggtcggtgc gatggcgtct
      421 ggcacgagca cactcctcgc tgagacgttg cagaaaatgc tttttgttgt acaagccggt
      481 gaggctgtcc gtcgtggcct ggtgcaaaag acgagagttg tagattgcct gcgaggccat
      541 ggtcgcgaac acgcgcagga gctcctggac ccgttcggta aaggtgttgg ctgacagccg
      601 attgtccagg tagagcacgc cgatcacgtt gttctgaatg cgaagagggg tgaccgcgat
      661 cgacagaatc ttgttgatca cgatactggt gttgcccgcc accagtggat caacactggt
      721 atcacggatc acgagcgttt caccggtgag aaaacatttc tgaatcaggc tctcggaggt
      781 ctgcatgtct ttctcgacgt cctgaagccc catgcgccgc accaccgcgc tttcgaaacg
      841 ctgtgtgttt tcgttgaaca ggatcaggcc tccgcgctcg gcaccagtca cctgcaaggc
      901 catgtcaatg gtgtattcga gcagacgttg aaagctgcga atcgacccga gcgcaaagct
      961 cacttcgaga atcgcactca gctcgcgaat gcggctgctg ataactgttt ccgtggcttg
     1021 cagatcatcg cgggcagttg gcggaatctt gtcgcggagc tgcgcgaaag tgtcgcgaac
     1081 cagattgacg ttgcgattga gaaagaaggg aaagacctgg acctcagtgc cgggctccga
     1141 cgttgccgcg agcgtctcgt tccctttgtc cccatctgcc tcgatggcga tatcgcgttc
     1201 gagtgtcaga aagacatcct gcgccaattg aactcgactg ccggtgacca gcggtacgaa
     1261 gaccccggct tgaacccgat gcccattgac atgggtgccg aacgtgctgt gcagatcctg
     1321 aatcgaagcc cccgagtcgg gagagtatcg taccagcacg tgctcgcgag aaatggcaat
     1381 ggaagtgagt ttgatggtgc agccttccgc acggcc
//
LOCUS       DAORHD010001425         1416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127655_length_1416_cov_2.515062, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001425 DAORHD010000000
VERSION     DAORHD010001425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127655_length_1416_cov_2.515062"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1205
                     /locus_tag="PKO06_22290"
     CDS             177..1205
                     /locus_tag="PKO06_22290"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72456.1"
                     /translation="MIDKLNIENFTVFKKAEFTFSPGLNVIIGENGTGKSHLLKLGYC
                     YLRALHDRQNDVGKPPSASMVLAITKRLYDVFKPEALENLVRRGNGKTTELRFTTRGE
                     NSEISIGANHTECLGKGLAQEVPPPLFIPPKEVLSFFYGFTSMYDLREVSIDGTYNDL
                     CLSIGLPELRDKHREKVTPLIHELEEAIGGRIVIEEGRVYFQPTKGEKMEANLLAEGW
                     RKIGMVALLIRTGSIATGKSVFWDEPEANLNPRQISLLAKVLVALAKNGVQIFLATHS
                     LYLTRELVIQDTLMKNGGASPRFFALSRKKDQVNVSQGNSIEDIDPLSFLDAELEQSG
                     RYLDLDKH"
     gene            1207..>1416
                     /locus_tag="PKO06_22295"
     CDS             1207..>1416
                     /locus_tag="PKO06_22295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72457.1"
                     /translation="MPTITEGDLTFAFPAGWQVTAYDQWSYYRNQFSNVGGEYKKGIK
                     GVDILAKDPQNTTWLIEVKDYRRSCR"
BASE COUNT          358 a          423 c          359 g          276 t
ORIGIN      
        1 gaagcaatcc gccagctcgc ctaccgactc tacactctct gcgaacgcag tagtcgcgcc
       61 gaagatgccc gctattacaa cgaactcatc acctcctggc ccggcatcga atccgccgcc
      121 agcaccatag gccgcgccgg cacccagaaa gatctgtttt gaaagcgagc tgacccatga
      181 tcgacaagct gaacatcgaa aacttcacgg tcttcaagaa agccgagttc accttctccc
      241 ccggcctcaa cgtcatcatc ggcgaaaacg gcaccggcaa aagccacctg ctcaagctcg
      301 gctactgcta cctgcgcgcc ttgcatgacc gccagaacga tgtcggcaag cccccgtctg
      361 caagcatggt tctcgccatc acgaagcgac tgtacgacgt tttcaaacca gaggccctgg
      421 aaaacctcgt tcgccggggc aacggcaaaa ccaccgaact gcgctttacc acacgcggcg
      481 agaattctga aatatcaatc ggagcaaacc acaccgagtg cctcggcaaa gggttggcgc
      541 aagaggtccc gccacctctt ttcataccgc cgaaagaagt cctgtcgttc ttttacggtt
      601 ttaccagtat gtacgatctt cgcgaggtca gcatcgacgg cacatacaac gatctctgcc
      661 tgagcatcgg tcttcccgaa cttcgcgaca agcatcgcga aaaggtcacc ccgttgattc
      721 acgaactcga agaggcaatc ggtggtcgga tcgtcatcga agaggggcgc gtctactttc
      781 agcctacgaa gggtgaaaag atggaggcca acctgcttgc ggaaggttgg cggaagatcg
      841 gtatggtcgc cctgctcatc agaaccggca gtatcgcaac cggcaagtcc gtgttctggg
      901 atgaacccga ggcgaacctc aacccgaggc agatttctct tctggcaaaa gtcctggtcg
      961 ctctcgcgaa gaatggtgtt cagatctttc ttgccaccca cagcttgtat ctgacgcgcg
     1021 aattggtcat tcaggatacg ctcatgaaga acggtggggc gagcccgcga ttcttcgctc
     1081 tcagcagaaa gaaagaccag gtcaatgttt cgcagggtaa ctcaatagag gacatagacc
     1141 cactctcatt ccttgacgct gaattagagc agtcgggtcg ctatctcgac ctggacaaac
     1201 actgatatgc caaccatcac tgaaggggac ctcactttcg ctttcccggc gggctggcag
     1261 gtaaccgctt acgatcagtg gtcatactat cgaaaccagt tcagcaacgt cggcggcgaa
     1321 tacaagaagg gcatcaaggg cgtcgatatc ctcgccaaag acccgcagaa taccacgtgg
     1381 ctgatagaag tgaaggatta ccgaagatcc tgcaga
//
LOCUS       DAORHD010001426         1415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127816_length_1415_cov_2.134559, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001426 DAORHD010000000
VERSION     DAORHD010001426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127816_length_1415_cov_2.134559"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PKO06_22300"
     CDS             complement(<1..1196)
                     /locus_tag="PKO06_22300"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNV72458.1"
                     /translation="MSDTGYKTWSERLGIAPGEGLITLASGVYFLLIILVYYVLKPLR
                     EGLALEVGSDNVPLLNVLSMLSLIPANALYSLIVSHFRRDVFIPTLTRLCGLSLVVFW
                     YIFRDTAVAEARLESLASPHALAIAGYYIWVNLFGLFMVSMFWSFMNDIFSLSQGRRL
                     YTLIGYGGCIGGLGGGFLTSQLVHLLGAANLFLVVVVLLEPTIWLMRYIHRQSQILPN
                     REHKTVEIAAVGSDSSPQETGWRRELNYSLAGVRLTFSSLFVGLMALEMFLYTFGSTL
                     FSYQVNSLMENTIPTRSERTIYWAHLYNYINGLSLLTQFCLTQFTLRSSKPWNGLLLM
                     PLSQVVGSIALLFNPVLGIAAATGVIRYAINYSTGRAVRELFFTPLTREEKYQAKGFI
                     DTLIFRT"
BASE COUNT          384 a          350 c          411 g          270 t
ORIGIN      
        1 gtgcgaaaaa tcagggtgtc gatgaacccc ttggcctgat acttctcttc ccgtgtgagc
       61 ggggtgaaaa acaactctcg aactgcccgc ccggtggaat aattgatggc ataccggata
      121 acgccggtcg cagcggcaat gccgagaacc gggttgaaca ggagtgctat cgaaccgacc
      181 acttgactca agggcatcag gagcaggccg ttccagggtt tggaggaacg aagggtaaac
      241 tgcgtcagac aaaactgtgt cagtaaagac aggccgttga tgtaattgta caagtgagcc
      301 caatagattg tgcgctcgct gcgcgtgggg atggtgtttt ccattaacga gttcacctga
      361 tacgaaaaaa gggtgctccc aaacgtgtag aggaacattt cgagtgccat caggccgacg
      421 aacagcgacg agaaggtcag tcgaactcct gccagtgaat agttgagttc tcgtcgccac
      481 ccggtttcct gaggggagga gtcacttccc acagctgcga tttcgaccgt cttgtgctct
      541 cgattgggca gaatctgtga ttggcgatgg atgtaacgca tgagccagat ggttggttca
      601 agcagaacca ccaccacaag aaacagattg gcagcaccaa gcagatgtac gagttggctg
      661 gtcaggaaac ctccccccag acctccgatg catccgccgt atccgatcag agtatacagc
      721 cgacgtccct gagacagaga gaaaatgtcg ttcatgaacg accagaacat gctgaccatg
      781 aaaagaccga agagattgac ccagatgtag tatccggcaa tagccagagc atgcggggag
      841 gccagacttt cgaggcgagc ttcggctacg gctgtgtccc ggaaaatgta ccagaacacg
      901 accagactca gtccgcaaag ccgggtaagg gttggaatga atacatcacg tcgaaagtgg
      961 ctgacgatca acgaatacag agcattggcc ggaatgaggg aaagcatcga aaggacgttg
     1021 agcaggggca cgttgtctga acctacttcg agcgccagac cttcacgaag tggtttgagg
     1081 acgtagtaaa ccaggatgat cagcaggaag tagacacctg atgccagggt aatgagcccc
     1141 tcacctgggg cgatgcccag gcgctcagac caggtcttgt aaccggtgtc actcaccgca
     1201 gacttctttc tgcgagcggc agagccgaaa aacggcacgc tctgcgcctg cttcgacggg
     1261 gttcaatgcc ggaccaagtg gaaaacaaga tgatcgcgga gtaggtccga gggaaattca
     1321 ccacattcct gaagaaaagg caactctttt actgatctga aaaggaaaag cccccggaaa
     1381 acttccgggg gcgtggggga actttgctga ttagc
//
LOCUS       DAORHD010001427         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_127938_length_1414_cov_2.446652, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001427 DAORHD010000000
VERSION     DAORHD010001427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127938_length_1414_cov_2.446652"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PKO06_22305"
     CDS             complement(<1..263)
                     /locus_tag="PKO06_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72459.1"
                     /translation="MLKKLLALMLVIVALVSLMPRTGQAQDSRGFFRDGQDNAGLERR
                     SGFRYPLPVQQIPFISYVASGTQTVGTATAVRIATLAADARAV"
     gene            complement(277..1272)
                     /locus_tag="PKO06_22310"
     CDS             complement(277..1272)
                     /locus_tag="PKO06_22310"
                     /inference="COORDINATES: protein motif:HMM:NF015802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HNV72460.1"
                     /translation="MDTLFKSSVITAAINAMKAVKTPVLDKVFARKLPQLSPNFAWDI
                     ETSHERILRNLKTGEPAQVTKKLRRARVSCEAPRFAPKRSISGDDMAELRKFGDAALP
                     ELLANRINRELADMRNEIDRTREYMAVRSILGQVIDSEGTVLVDYSFSGAQKPVLTGK
                     QLWTDSESNPLKNIRAWKKLIAKSVGGVDAFIAFCGSAAMDALIDNQKIQNLLQFQAG
                     QQLAEKGRIAFLAETEIEEYFGYYKDSNGDAQDMIPENEFVLVGVSAETLAELYAPVI
                     DFKDPAGVGKGQLASMFFAKSWEEEDPSVRWLKAESRPLPVLFRPECVVRARVVA"
BASE COUNT          279 a          446 c          417 g          272 t
ORIGIN      
        1 tcgaccgctc gagcatcagc cgccagggtg gcaatgcgca ccgccgtcgc cgttcccacc
       61 gtctgcgtgc ccgaagcaac atacgagatg aacgggatct gctgcaccgg cagcgggtat
      121 cgaaacccgg agcggcgttc cagacccgcg ttatcttgac catcgcggaa gaacccgcgc
      181 gaatcctgcg cctgacccgt gcgcggcatc aggctgacca gggcgacgat aaccagcatc
      241 agagcaagaa gttttttcaa cattcgagtg gcctccttac gccacgactc gcgcccgaac
      301 aacgcattcg gggcggaaca gaaccggcag cgggcgcgat tcggccttga gccagcgaac
      361 cgagggatct tcctcttccc agctcttggc aaagaacatc gaggccagct ggcccttgcc
      421 gacaccggca gggtctttga aatcgatgac cggggcatac agctcggcca gggtttccgc
      481 cgacacaccg acgagcacga actcgttttc cgggatcatg tcctgggcgt cgccgttgga
      541 atccttgtaa tagccgaagt attcctcgat ctcggtctcg gccagaaacg cgatgcgccc
      601 cttttcggcc agctgctgac cggcctgaaa ctgcaagagg ttctggatct tctggttgtc
      661 gatcagcgca tccatcgccg ccgagccgca gaaggcgatg aaggcatcaa cgccgccgac
      721 cgacttggcg atcagcttct tccaggcgcg aatgttcttg agcgggttcg attcgctgtc
      781 ggtccacagt tgcttcccgg tcagaacggg cttctgagcg ccggaaaagc tgtaatcgac
      841 cagcacggtg ccttccgagt cgatgacctg accgagaatg gaacgaaccg ccatatactc
      901 gcgggtgcgg tcgatctcgt tgcgcatgtc ggccagttcg cggttgatgc ggttggccag
      961 cagttcaggc aatgcggcat caccgaactt gcgcagttca gccatgtcgt caccgctgat
     1021 gctgcgtttc ggagcgaagc gcggagcctc gcacgagacg cgggcgcggc gcagtttctt
     1081 ggtcacctgg gcgggctcac ccgttttcag gttccgcaga atgcgctcat gactggtctc
     1141 gatatcccag gcgaagttcg ggctcagctg cggcagcttg cgcgcgaaca ccttatcgag
     1201 aaccggggtt ttgaccgcct tcatcgcatt gatggcggca gtgatgacag aagatttgaa
     1261 aagggtgtcc atgattactc ctcacctacg aagattccgg cggcatacag atccgcctcg
     1321 ccgttggcgt cgagcccgac cagatcttcg cgcacgacgt cgccatgaac caacaccaac
     1381 gcctcgactg ccgccgcatg cacgggcacg tctt
//
LOCUS       DAORHD010001428         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128222_length_1412_cov_2.555637, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001428 DAORHD010000000
VERSION     DAORHD010001428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128222_length_1412_cov_2.555637"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PKO06_22315"
     CDS             complement(<1..966)
                     /locus_tag="PKO06_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72461.1"
                     /translation="MKRFVLVALLALLPVCTFAADTPVKAVMINGLRYEASLASQAVA
                     PKTLLEIALGTGKENVGSDEQDAEHRTEGAPYAFCALKDGSVWVLDTINQKLKHFSAE
                     GKCLTEVAFPEHPEKFARIRDFAIGPKESFYFLCSTDGKVERVDGQGKLITEIEGLSD
                     VWEIGADPKGNVLVKNPAMNALMRFNPEGELIESYDGQADYSVYTDADGHPYGVRSDD
                     KNAVLFKLTAASPTASIDLAKFPLELAAGNKAHYYSARVCGVDQTGTIYLELIAGDDD
                     GVIHQNRLLKVSPDGKTLGKLEMLNTPFMAPDLPRHRVVMPDGRHE"
     gene            complement(987..>1412)
                     /locus_tag="PKO06_22320"
     CDS             complement(987..>1412)
                     /locus_tag="PKO06_22320"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV72462.1"
                     /translation="DATITPGQGRFGAAPCSPMPSRSSSEYGPRDLFGHSFHSGIDLP
                     VPTGTRLNALGDGVVTAVGYEAGGGHFIKIRYDNGVESFYCHLKSQSVRQGQRVSMGQ
                     EVARSDNTGQWTTGAHLHMGIKVNGQYVNPRSVRGIPLP"
BASE COUNT          281 a          393 c          434 g          304 t
ORIGIN      
        1 ttcatggcga ccgtcgggca tgaccacgcg gtgtcgggga agatcgggcg ccatgaacgg
       61 agtgttcagc atttcgagct tgccgagagt tttcccatcc ggcgagactt tcaagaggcg
      121 gttctgatga atgacgccgt catcatcgcc ggcgatcagt tccaggtaga tagtgccggt
      181 ttggtcgacg ccgcagacgc gagcgctgta gtaatgggct ttgttgccgg cggccagttc
      241 gagcgggaac ttggccaggt caatcgaggc tgtcggtgaa gcggcggtga gtttgaacag
      301 gacagcattc ttatcgtcag accgcacgcc gtacgggtgc ccgtcggcat cagtgtagac
      361 cgagtaatcc gcctgaccat cgtaggactc gatgagttct ccttcggggt tgaagcgcat
      421 cagagcgttc attgctgggt tcttcaccag gacatttccc ttcgggtcgg caccgatttc
      481 ccacacatcg ctcaggcctt caatctcggt gatcagtttg ccttgtccat cgacgcgttc
      541 aaccttgccg tcagttgagc acaggaaata gaaactctct ttcggcccga tggcgaagtc
      601 gcgaatgcgc gcgaactttt cgggatgctc gggaaaggcc acttctgtca gacattttcc
      661 ttccgccgaa aaatgcttga gtttctgatt gatggtgtcg agaacccaga ccgaaccgtc
      721 tttcagcgcg cagaaggcat acggagcgcc ttcggttcga tgttccgcgt cctgctcatc
      781 gctgccgacg ttctctttcc ccgtgccgag cgcgatctcg agaagggttt tgggcgcgac
      841 cgcctgggac gcaagcgatg cctcgtaacg caggccgttg atcattaccg ccttcacggg
      901 cgtgtcggct gcaaaggtgc agacaggaag cagagcgagc aatgcaacga gaacgaacct
      961 tttcatgacg atctctccaa acctcttcag gggagtggga tgccgcgtac actgcgggga
     1021 ttcacatact gaccattcac tttgatgccc atgtgcaggt gagcgccggt ggtccattgt
     1081 ccagtgttgt ccgagcgggc gacttcctgg cccatcgaga cacgctgacc ctggcgcacg
     1141 ctctgcgatt tcagatggca gtagaacgat tcaacgccat tatcataccg gattttgatg
     1201 aagtgaccac cgccggcttc gtagccgacc gccgtgacga cgccgtcgcc gagcgcgttg
     1261 aggcgggtgc ctgtgggaac cggcaaatcg atgccgctgt gaaagctgtg accgaagaga
     1321 tcacgggggc catactcgga agaagagcga ctgggcatgg gtgagcaggg agccgcaccg
     1381 aaacggccct gcccgggagt gatggtggca tc
//
LOCUS       DAORHD010001429         1411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128327_length_1411_cov_3.393805, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001429 DAORHD010000000
VERSION     DAORHD010001429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128327_length_1411_cov_3.393805"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKO06_22325"
     CDS             complement(<1..272)
                     /locus_tag="PKO06_22325"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV72463.1"
                     /translation="MRKIGRAVRGLISIGVLTTWTLATLFAHWLLPLRFDLCLLFGAV
                     MTVTGPTVILPLLRQIKPDPKVAAILKWEGILVDPVGATLAVLVFEV"
     gene            complement(290..580)
                     /locus_tag="PKO06_22330"
     CDS             complement(290..580)
                     /locus_tag="PKO06_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72464.1"
                     /translation="MFDLFSRVMTQMHHSAFLLYQAASTQIMFNLALILLLGIGAQWL
                     AWVFHAPSILLLLLFGFAVGPGLGLVNPDQLLGQLLTPIVSLRSPSFFSRAA"
     gene            831..1301
                     /locus_tag="PKO06_22335"
     CDS             831..1301
                     /locus_tag="PKO06_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72465.1"
                     /translation="MFLAAALAIQSLRIWTPVIGVLINALMVVLYYRGGLQGALLLAV
                     ATPIGAAIVGVLPVIFIGMVPVLAVGNALLIWVYHSLSQRGAVIRVLVPSLGKSLFIG
                     LAGYGVLSAFAIPEALKPLSLVLLGQQFFTAGIGILFGERVCRAAGVALARATG"
BASE COUNT          294 a          397 c          437 g          283 t
ORIGIN      
        1 acttcgaaca ccagcacggc gagggtggcg ccgaccgggt cgacgagaat cccttcccat
       61 ttcagaatgg cggcgacttt ggggtcaggt ttgatctgac gcagcaacgg aaggatgacc
      121 gtgggcccgg tgacggtcat gacggctccg aagagcaggc agagatcgaa acgtaacggt
      181 agcagccagt gagcgaacag cgtggcgagt gtccaggttg tcagaacgcc aatgctgatc
      241 agtccccgaa cagcgcggcc aatcttgcgc aaatcagata ctttcaggtt caggccgccc
      301 tcgaaaagaa tgacggcgac cgaagcgaca cgatcggggt gagcagttgg ccaagcagct
      361 ggtcaggatt caccagaccg agaccggggc cgaccgcaaa gccgaacagc agcaagagca
      421 gaatcgacgg cgcgtgaaac acccaggcca gccactgcgc gccgatcccc agcagaagaa
      481 tcaacgccag attgaacatg atctgggttg atgcggcttg atacagcaga aaggcactgt
      541 gatgcatctg ggtcatcaca cgggaaaaca aatcgaacat gagccgcctt tcaccaccaa
      601 aaagtctgtc ttcagtatcc gcaatctgtg tccgacaatc aacccttctc tcggaaaatc
      661 cccgatgatc aagagctgac gtagagcggg gcgagtgtga tagaatcaac cgctttctca
      721 gtcgagaccg ctgaagagcg ttacgtattg aacccagact tcttctgtcc aacttcaacc
      781 tttccgcgcc accagcgacg tcacgtctgc gcacggtgac tctcgccggc gtgtttctgg
      841 ccgcggcgct ggcgatacag tcgctgcgca tctggacgcc ggtgatcggc gtgctgatca
      901 atgcgctgat ggtggtgctg tattatcgtg gcgggttgca gggagctctg ttgctggcgg
      961 tggcaacccc catcggcgcg gccatcgtcg gagtgctgcc ggtgatcttc atcggcatgg
     1021 taccggtgct tgccgttggg aacgccctgt tgatctgggt ataccatagt ctctctcaac
     1081 gtggagccgt gatacgagtc ctggttccgt cacttggaaa gtcgctgttc atcggtttgg
     1141 ccgggtatgg cgtcttgtcc gcgttcgcga ttcccgaagc attgaagccg ctttctctgg
     1201 tgctgctcgg gcagcagttc tttaccgccg gtatcggcat tctcttcggt gagcgggtgt
     1261 gtcgagccgc gggagtggcg ctggcacgcg cgacgggctg aggcggcagc ggtgtcggtg
     1321 aggtttcacg acgagcttcc tctcccgggc atctcggtga tcatgccggt tcgaaacgcg
     1381 gcatcaacgc tgcgagaagc gattgacagc c
//
LOCUS       DAORHD010001430         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128534_length_1410_cov_2.466421, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001430 DAORHD010000000
VERSION     DAORHD010001430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128534_length_1410_cov_2.466421"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..146)
                     /locus_tag="PKO06_22340"
     tRNA            complement(74..146)
                     /locus_tag="PKO06_22340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Val,seq:tac)
     gene            complement(235..732)
                     /locus_tag="PKO06_22345"
     CDS             complement(235..732)
                     /locus_tag="PKO06_22345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72466.1"
                     /translation="MSIRRPRGIIRTTAAIQSAPPQGKGQLLSPRSEVTYQIFEDAYY
                     EKRVWMPSANLSNYVMIFTSLSLGHKSRLGRLAFRHLLQTLSESEFVPRILVFWNHAV
                     KACVEGSPLLPCLARIERAGVKILVCTFALEKLKLKSKLRSGKLASNIDLLDAIHKAQ
                     KVVSF"
     gene            877..>1410
                     /locus_tag="PKO06_22350"
     CDS             877..>1410
                     /locus_tag="PKO06_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72467.1"
                     /translation="MFKRLALVLGVLFLVGVAVSASAQDSVLLQYNPKPGTTTKYKMH
                     INGNTVVTAMQRAQKTDLETDMTIEQKVTGVDKSGNVDMTTEIKEGTITVNKTPTQLP
                     AIGQVIRVKMAKNGEVLESEGMDNQSNFQQMQIKFPDKPVKVGDAWEAVISPNPQLPI
                     PLKVKYTFLGKETVDGFE"
BASE COUNT          335 a          371 c          399 g          305 t
ORIGIN      
        1 cgcgacctcc tgcgtgacag gcaggcgttc taaaccgact gaactacatc cccaatcgat
       61 ctgttacggg cggtgggcgg tgccggactt gaaccgacga ccacctgcgt gtaaggcagg
      121 cgctctacca gctgagctaa ccgcccgtgt tcaggctatc aagataccat aaccccgtga
      181 tgctgtcaaa cacttttttg taccccgggt tcttcggtga aaacgcgggc caggtcagaa
      241 gctgacgact ttctgggcct tgtggatggc atcgagaaga tcgatgttgc tggcgagttt
      301 cccagagcgc aatttgctct tgagtttcag tttctccagg gcgaacgtac aaaccagaat
      361 tttaacgccg gctcgctcga tgcgtgccag gcaggggagc agcggactcc cctcgacgca
      421 ggccttgacc gcgtggttcc agaagacgag gatccgggga acgaactcac tctcggaaag
      481 ggtttgcagc agatgacgaa aggccagtcg cccgagccgg cttttgtgac cgagcgagag
      541 ggaggtgaaa atcatgacgt agttggaaag attggccgag ggcatccaga cgcgcttctc
      601 gtagtaggcg tcttcgaaga tctggtaggt gacttcagaa cggggcgaca gaagttgacc
      661 tttcccttgc ggcggggctg attgaattgc ggcagttgtc cgtatgatac cccgaggccg
      721 gcgtattgac aagacattaa tattgcttga cacctctggg tcactatggt atcgtttgcc
      781 tgtcttgggc tctatgagct tctttccgaa gacagtgttc caagacagga tacgtacaag
      841 ttttacagac atacgatgcg aaaggagtct gtgttcatgt tcaaacgtct ggctctcgtt
      901 ctcggtgttc ttttcctggt tggtgtcgcc gtcagcgctt cggctcagga tagtgttctg
      961 cttcagtaca acccgaagcc gggcaccacg accaagtata agatgcacat caacggcaac
     1021 acggtcgtca ctgccatgca gcgcgctcag aagacggatc tcgaaaccga catgaccatc
     1081 gaacagaagg tcactggtgt cgacaagagc ggcaatgtgg atatgaccac ggagatcaaa
     1141 gaaggcacga tcactgtcaa caagactccc acccagctgc cggccattgg tcaggtcatc
     1201 cgcgtgaaga tggccaagaa tggcgaagtg ctcgaatccg aaggcatgga taatcagagc
     1261 aacttccagc agatgcagat caagttcccg gacaaaccgg tcaaagtcgg tgacgcctgg
     1321 gaagccgtga tcagccccaa tcctcagctg ccgattcctc tgaaggtcaa atacaccttc
     1381 ctcggcaaag aaaccgttga tggttttgag
//
LOCUS       DAORHD010001431         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128556_length_1410_cov_2.140221, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001431 DAORHD010000000
VERSION     DAORHD010001431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128556_length_1410_cov_2.140221"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKO06_22355"
     CDS             complement(<1..345)
                     /locus_tag="PKO06_22355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV72468.1"
                     /translation="MFDNNVLKLTQLPVDSKKVALSCDLGGGQWVDMNYLEFKEIIDE
                     VTKVFMMIGLKKNDRVLIISENHYKWLPVFLGITGFGAVAMPIDGGIVDSRLLAIIEN
                     SQPRAIIVSKKYQ"
     gene            complement(373..>1410)
                     /locus_tag="PKO06_22360"
     CDS             complement(373..>1410)
                     /locus_tag="PKO06_22360"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNV72469.1"
                     /translation="GLNMPHRKNLKIAIVINEMSRGGSTKEFLQGNLYKLIESFQKDV
                     GHQIAILLTRSRDHLFKSIISMQNQGYNTFLTYGGDGTINDSIQNLNEGTFLIPISAG
                     NANDFAGRLNVRNTSDIQLAFEDVLAMVEDLIHGRVNVIGLDVGEIQFLDGSGKLCRK
                     RFVNNCGFGVTAQTVKQIEGQVKKNYALSGLQAIMSASAMDLSYLTAFSDEEATVKCL
                     GVEALLCRKVGNYAFMAPFKRENNEAMHFFVINDMSWWFRLMFVLAIKFGTWATLFGM
                     GSYFHDSRRKDKPSKYSMTIEGLSHLTMKLNELVPLHVDGNMVPEFEETIQKQFTVRI
                     LPKFLKTIAPC"
BASE COUNT          326 a          374 c          367 g          343 t
ORIGIN      
        1 ctggtatttc ttcgacacga tgatagcgcg gggttgtgag ttctcgatga tggcgagcag
       61 acgcgagtcg acgattccac cgtcgattgg catagccact gcaccaaacc cggtgattcc
      121 gaggaacacg ggcagccact tatagtggtt ctcgctgatg atcagcaccc gatcgttctt
      181 cttcaagccg atcatcatga agaccttggt gacttcgtcg atgatttcct tgaactcgag
      241 atagttcatg tcgacccact ggccaccgcc cagatcacac gacagcgcga ccttcttcga
      301 gtctacgggt agttgggtga gcttcaatac attattgtcg aacatcctct cctccttgaa
      361 cgggggctgg ggtcagcatg gagcaatcgt tttcaggaac ttgggcagaa tgcgaacggt
      421 gaactgcttc tgaatcgtct cttcgaattc tggaaccatg tttccatcaa catgtaatgg
      481 taccaactcg ttaagcttca tcgtcagatg tgaaaggcct tcgatggtca tcgaatactt
      541 gctcggctta tccttgcggc gactgtcatg gaagtagctg cccatgccga acaaggtcgc
      601 ccaggtgccg aacttgatag ccagcacgaa catgaggcgg aaccaccacg acatgtcgtt
      661 gatgacaaaa aagtgcatgg cctcattgtt ctcgcgcttg aacggcgcca tgaacgcata
      721 gtttccgact tttcgacaca acagagcttc gactccgaga cactttaccg tcgcctcttc
      781 gtcggaaaaa gcggtgagat agctgagatc catggcagag gcggacatga tcgcctgcag
      841 gcccgaaagc gcatagttct tcttcacctg accttcgatc tgtttcacgg tctgcgcggt
      901 cacgccaaac ccgcagttat tgacgaagcg cttgcgacag agctttcctg agccgtcgag
      961 aaactgtatc tctccgacat cgaggccgat gacgttcacc cggccgtgga tcaggtcttc
     1021 caccatggcc agcacgtctt cgaacgccaa ctgaatgtcg ctcgtgttac gaacgttcag
     1081 acgaccggca aaatcattgg cattaccggc cgaaatggga atcagaaagg tgccctcatt
     1141 gagattctga atcgagtcgt tgatcgtgcc gtcaccaccg taggtgagaa aggtgttgta
     1201 tccctgattc tgcatcgaga tgatgctttt gaagaggtga tcgcggctgc gggtcagcag
     1261 aatggcgatc tgatggccga catccttctg aaacgattcg ataagcttgt acaagtttcc
     1321 ctgcaggaat tccttggtac taccgccacg agacatctca ttgataacga tggcgatctt
     1381 gaggttcttg cgatgaggca tgttcaggcc
//
LOCUS       DAORHD010001432         1410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128571_length_1410_cov_1.915129, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001432 DAORHD010000000
VERSION     DAORHD010001432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128571_length_1410_cov_1.915129"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PKO06_22365"
     CDS             complement(<1..333)
                     /locus_tag="PKO06_22365"
                     /inference="COORDINATES: protein motif:HMM:NF017541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HNV72470.1"
                     /translation="MLDSLGLSTSEWANIARQSPWHALRMNLNTFLRHGVFADSELVA
                     FVARRLRDRDDIHRNRVFPYQLLAAYRNVNVEMPGEIKDALQDAMEIAISRVPAEVDP
                     CAPSCVVQS"
     gene            complement(367..1017)
                     /locus_tag="PKO06_22370"
     CDS             complement(367..1017)
                     /locus_tag="PKO06_22370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72471.1"
                     /translation="MNKHLFSTSPGEFTPPADCINEAGGLAYALPPHQALAQLAATGC
                     LGHTYYATAETQLSQVLELCKLVEPEFVARVAVYGRNRGYMKDMPALLCAWLTTVDLE
                     LAKKVFERVIDSGKMLRNFVQVMRSGVIGRKSLGTAPRRFVREWFRQRDDDTIFRQSV
                     GQSPSLGDIVKMVHPRPETKARAALFAWLAGLKHDSQELSGLAREYELYKTGKADG"
BASE COUNT          316 a          424 c          380 g          290 t
ORIGIN      
        1 gctctgcacc acgcaggatg gcgcgcaagg gtcgacctcg gccggcacgc gggagatcgc
       61 aatctccatc gcgtcctgca gggcatcttt gatttcgcca ggcatctcga cgttgacgtt
      121 ccgatacgcc gccagcagtt gataagggaa aaccctgttt cgatgaatgt cgtccctgtc
      181 gcgcaagcgc ctggcaacga aagccaccag ctctgaatca gcaaacaccc catgtcgcaa
      241 gaacgtgttc aggttcatgc gcagagcgtg ccagggcgat tggcgagcga tgttcgccca
      301 ctcactggtc gagaggccca gagaatcgag catctgtaag ggaacagctg gtactgcaac
      361 acctgtctag ccatccgcct ttcctgtttt gtacaactca tactctcgcg ccagcccaga
      421 aagctcttgc gagtcatgct tcaaacccgc gagccacgcg aacaacgccg cacgagcctt
      481 tgtctcaggc ctgggatgaa ccatcttgac aatgtcgccc agcgagggtg actggcccac
      541 cgactgtctg aagatcgtgt catcatcgcg ttgtctgaac cactctctga cgaatcgtcg
      601 aggtgctgtg ccgagagact tccgcccgat gacccccgaa cgcatgacct gaacgaaatt
      661 gcgcagcatt tttcccgaat cgatgacgcg ttcgaacacc ttctttgcca gctcgaggtc
      721 aaccgtcgtc agccacgcac acaagagggc tggcatgtct ttcatgtatc ccctgttgcg
      781 accgtacacc gccaccctgg cgacaaactc tggttcgacg agcttgcaca gctcgagaac
      841 ctgagacaac tgggtttccg ccgtcgcata gtaggtatga cccagacacc ccgttgccgc
      901 caactgtgcc agcgcctgat gagggggcag ggcataggcc agaccgcccg cttcattgat
      961 gcagtccgca ggaggagtga attcccctgg agaagtggaa aacaaatgct tgttcatggt
     1021 ggaagcgcct ccattcgtga tgtgcgagag ttccggtatc agctcatctc aaacggtgtg
     1081 ccaacgggaa gcgcttacgt gccgaccatt tccattctcc tgtttcgcgc gattcgacga
     1141 ctgcggttca tgcatacata ttccggaaaa ttctcaggca ccggcaatgt tcgcgaaact
     1201 gagagtactc gttggcccga cgcgaacagc ccatttttcc ggaatgtgcg tgttgcagca
     1261 gcacgagagg aaaggaactg acatgacgga tcgaatcaac aacgcatcgc ggagtggcgg
     1321 gttcattgtg accacctgtt caccagatcg taaaaccagg cgcaggcaga cgcgatccat
     1381 cagcacctgg ccttactgcg ttctgcgtga
//
LOCUS       DAORHD010001433         1407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_128990_length_1407_cov_3.089497, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001433 DAORHD010000000
VERSION     DAORHD010001433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128990_length_1407_cov_3.089497"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PKO06_22375"
     CDS             <1..729
                     /locus_tag="PKO06_22375"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HNV72472.1"
                     /translation="TREAGGVVYAGLPRALHATSEYQLLENSSFWTGYSEYRQNPLWS
                     SYRLGLEEKGIPAGRISYFKSDSRVRNPVSHSDFAKTGFDRGHLAPNSGIATRYGEVG
                     QLETFVMSNVCPQAPDLNRKLWETIERLEDDKWANSLGEVYVIAGPIFDETRQFLPAK
                     KRGDIEIPDAFFKVIVDETSTDARMLAFLIPQTVTGKERPDQFLVSVDEIERRAGLDL
                     FPALSDERENRLEAHAATELFPVN"
     gene            792..>1407
                     /locus_tag="PKO06_22380"
     CDS             792..>1407
                     /locus_tag="PKO06_22380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72473.1"
                     /translation="MMKKRQRIHWSAHERALLLTGWLMLVVSCGLWWSPVVLSAEPNS
                     AADPMGALRQGSAAEDPLSVLKRQQLGDSGVTAPGGESGPPPDLASGMTDIDLSPLSE
                     PESPLPPLSAEERRVWFERQGEQAQLMEQDLRDRLRDIADELKKEEPVLRQFGPTGAT
                     ETKALMMWYRWSGRFLNSRRLDLWSLIWAGNSPVPLITLVAAGWG"
BASE COUNT          279 a          392 c          456 g          280 t
ORIGIN      
        1 acgcgtgagg ccggcggggt cgtctatgcc gggttaccgc gtgcgctgca tgcgacgagc
       61 gaataccagt tgctcgagaa ttcctcgttc tggaccggct attcggagta tcggcaaaac
      121 ccactctggt catcgtaccg gcttggtctc gaagaaaagg gaattccggc cggacgcatc
      181 tcgtatttca agtccgattc ccgagtgcgc aacccggtca gtcactcgga cttcgccaag
      241 actggcttcg atcgcgggca cctggcgccc aattcaggga tagccacccg gtacggagaa
      301 gtcgggcagc tcgagacatt cgtcatgtcg aacgtctgtc cgcaggctcc tgacctgaac
      361 cgcaagctct gggagaccat cgaacgtctc gaagatgaca aatgggccaa cagtctcggc
      421 gaggtctatg tcattgccgg accgatcttc gatgagacgc gtcagtttct gccggcgaaa
      481 aagcgcggcg atattgagat tcccgacgca ttcttcaagg tgattgtcga tgaaacgtcg
      541 acggatgccc gcatgctggc gttcctgatt ccgcagaccg tgaccggaaa agagcgccct
      601 gaccagtttc tggtctcggt tgacgagatc gagagacgag ccggtctcga cctgtttccg
      661 gcactgagcg atgaacggga aaaccgcctc gaagcacacg cggcaacaga actgtttccg
      721 gtgaattgag ggtcccgatt tccgactgtc acagattggt gctcgcattc gtttgagaga
      781 gaagagaatc gatgatgaag aaacgacaac ggattcactg gtccgctcac gaacgggctt
      841 tgcttctgac cggctggttg atgctggtcg tgagttgcgg gctctggtgg tcgcctgttg
      901 tgctgtcggc tgagccgaac agcgcggctg atcccatggg agcgctgcgt cagggaagtg
      961 ccgccgaaga tccgctcagt gtcctcaagc ggcagcagct gggggacagc ggtgtcactg
     1021 ctccgggcgg ggagagtggg ccgccgccgg atctcgcgtc gggtatgacc gatatcgatc
     1081 tttcgcctct gtccgaaccc gaatctccgc tcccaccctt gagtgcggaa gaacggcgcg
     1141 tctggttcga gcgacagggt gagcaggcgc agctcatgga acaggacctg cgcgaccggt
     1201 tgcgtgacat cgcggacgaa ctgaaaaagg aagaaccggt gctgcggcag ttcgggccga
     1261 ccggagccac ggaaaccaaa gcgctgatga tgtggtatcg ctggtcgggg cggtttctga
     1321 acagccgccg tctcgatctg tggtcgttga tctgggcggg caactcaccg gtcccgttga
     1381 tcacgctggt ggcggccggc tggggag
//
LOCUS       DAORHD010001434         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129095_length_1406_cov_4.122872, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001434 DAORHD010000000
VERSION     DAORHD010001434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129095_length_1406_cov_4.122872"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..585
                     /locus_tag="PKO06_22385"
     CDS             163..585
                     /locus_tag="PKO06_22385"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV72474.1"
                     /translation="MRTIEAYVRQHQEILTLVTDVLGSLDRAAVQRHPSRVLEQIQRL
                     AQALRMHLAQEDLGVYPILLRHADVRVRETAQQFQDTMGGLSHQFEGFLERWKTSDTL
                     QAAPEPFIQETRDLFEALAKRILSEDTILYPLADNPGQ"
     gene            complement(641..1027)
                     /locus_tag="PKO06_22390"
     CDS             complement(641..1027)
                     /locus_tag="PKO06_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004403788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV72475.1"
                     /translation="MKVNLILYVTDQKMSSAFYQQVLQLAPVLDVPGMTEFSLTTDTV
                     LGLMPIAGIRRLLGDALPDPAQAAGVPRAEVYLTVDDPAAYHQRALGAGARELSPLLS
                     RNWGDEAAYSLDPDGHVLVFARRLKL"
     gene            complement(1159..>1406)
                     /locus_tag="PKO06_22395"
     CDS             complement(1159..>1406)
                     /locus_tag="PKO06_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNV72476.1"
                     /translation="ELLELAASLQRELQSWCDPKPFRPHLTLARRRAECARFVPADVS
                     REWLVTEFELIRSELRSAGPEYQTLQCFDLTSSGNTT"
BASE COUNT          302 a          420 c          382 g          302 t
ORIGIN      
        1 gcgcaagctt cgacatgatc cgcaacctgc acgaatcgac cttcggtcga tagatcgcga
       61 aagagtcgtc agtgagagcg ccggggttac ccggcgctct ctcttttcgt cgcggaaagg
      121 aagaaggttg gcattcagag ccaattctgc gataattttc ccatgagaac catcgaagcc
      181 tacgttcgcc agcatcagga aattctgacc ctcgtcacgg acgtgctcgg ttcccttgat
      241 cgagccgccg tgcagcggca tccgagtcgg gtgctggaac agatacagcg attggcccag
      301 gcattgcgca tgcatctggc ccaggaggac ctcggcgtgt atcccatctt acttcgtcac
      361 gcagatgtgc gcgtacgaga aaccgcacag cagtttcagg atacgatggg cggactcagc
      421 catcagttcg aaggttttct cgagcgctgg aagacttccg acaccttgca ggccgctccc
      481 gagccattca ttcaggaaac gcgtgatctt ttcgaggctc ttgccaaacg cattttgagc
      541 gaagatacca ttctgtatcc gctggcagac aatccgggtc agtgattcaa ccggtactca
      601 cgctcttgct ccggctgtcg taatctggca ggagcatcaa tcagagcttc agtcgtcgcg
      661 caaaaaccag cacgtgtcca tccgggtcca ggctgtaggc ggcttcatca ccccaattgc
      721 gagacagaag cggactcagt tcgcgtgctc ccgccccgag cgcacgttga tggtaggcgg
      781 ctggatcatc aaccgtcagg tagacctcgg ctcgcggcac tccggctgcc tgagcggggt
      841 caggcagcgc atctccgagc aagcgcctga ttccggcgat gggcatcaag ccaagcacgg
      901 tgtctgtggt cagagagaac tccgtcatgc ccggcacatc caacaccgga gccagttgca
      961 gcacctgttg atagaaggct gagctcatct tctgatcggt cacataaaga atcaggttca
     1021 ccttcatcgt gtttctccag acttgatttc ttgctgccag cactcttttt tcatgagctt
     1081 tgaagcagat accgactcgt accgaccagg ctcacgctca tcgccacgtc tcgacgggct
     1141 catcgtgcgg ggactatttc aggtcgtgtt ccctgaactc gtcagatcaa aacactgcag
     1201 tgtctgatac tcaggaccag ccgatcgtag ctcactccgt atcaactcga actcggtcac
     1261 cagccattca cgactgacat ccgctggtac aaatcgcgca cattcggcgc ggcggcgcgc
     1321 cagggtcaaa tgcgggcgaa atggttttgg gtcgcaccaa gactgcagct cacgctgcag
     1381 cgaggctgcc agttcgagca gttctg
//
LOCUS       DAORHD010001435         1405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129272_length_1405_cov_3.148148, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001435 DAORHD010000000
VERSION     DAORHD010001435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129272_length_1405_cov_3.148148"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1405
                     /locus_tag="PKO06_22400"
     CDS             <1..>1405
                     /locus_tag="PKO06_22400"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV72477.1"
                     /translation="RWMKELGQKAVIVSLEWSRIEPNPKQYDQAAIDHYREVFKQLRA
                     QGIEPIVVLWDRTLPSWVSAYTGLEVPGVILDFSDFAGQAAKQFGDLVDVWVPVRDPV
                     GAASKAFKHGVNPPGKTDVAAFSKAVISLLAMHRGAREALMANDTTAAGGKKACEVGL
                     IASMRCIRPNRAEHPMDQTLAKTSEHFSRAFVDAIMKGDLTVSSAPTDSGKGKGGFGK
                     MPVYTPAAPPSYDKRLADFLAIQYEGLEELKFNPLFPLFTQKIIPPGAVRDGAGQISF
                     AEGLTQLLASLKKYPVPMMVIVSAFDAKGFDRVAHLREHAAATLKAIGNGCAVKGLFY
                     DSLLSGFEYEYGYSLHKGLLRVNRQVQERKPTMVAKAFARLAKGAALHAPTDEDAADS
                     DTPAAPGGADQPPVPAQPGESAGADPSAQPPASSDSAEQPSLPAQPGESVGADPSAGP
                     GLVPGGADQPPRPATTAD"
BASE COUNT          272 a          434 c          431 g          268 t
ORIGIN      
        1 cgctggatga aagaactcgg tcagaaagcg gtcatcgtct cactcgaatg gagccgcatc
       61 gaaccgaacc ccaagcagta tgatcaggcg gcgatcgatc actatcgcga ggttttcaag
      121 cagttacgcg cccagggcat cgagccgatc gtcgtgttgt gggaccgcac cctcccttcc
      181 tgggtttctg cttacaccgg tctcgaggta ccaggcgtca ttctcgattt cagtgatttt
      241 gccggtcagg ccgccaagca gttcggcgac ctcgtcgatg tctgggtgcc cgtgcgtgac
      301 ccggttggcg cggcatcgaa agccttcaag cacggagtca atcctccggg gaaaaccgat
      361 gtcgccgctt tttcgaaagc cgtcatcagt ctgctcgcca tgcatcgagg tgcgcgcgaa
      421 gctctcatgg cgaacgatac caccgcggca ggcgggaaga aggcctgcga agtcgggttg
      481 attgcctcga tgcgctgcat cagacccaac cgcgcggaac acccgatgga ccagacgctg
      541 gcgaaaacgt cggagcattt ttcgcgcgcc tttgttgatg cgatcatgaa gggcgatctg
      601 accgtcagct ctgcaccgac cgattcgggg aaaggcaagg gcgggttcgg caagatgccg
      661 gtctatactc ccgcggctcc cccgagctac gacaagcgac tggcggactt tctggccatt
      721 caatatgaag ggctcgaaga actgaagttc aacccgttgt tcccgttgtt cacgcagaag
      781 atcatacctc cgggcgctgt gcgtgacggc gcagggcaga tcagtttcgc tgaagggttg
      841 actcagcttc tcgcgagtct gaagaagtat ccggttccga tgatggtgat cgtttccgcg
      901 tttgacgcca aggggttcga tcgagtcgcc cacctgcgcg aacatgcggc cgccacgctg
      961 aaagcgatcg gtaacggatg tgccgtgaaa ggtcttttct acgacagcct gctcagcggg
     1021 ttcgagtatg agtatgggta cagcctgcac aaggggctgc tgcgtgtgaa tcgtcaggtg
     1081 caggaacgca aaccgactat ggtggccaag gctttcgccc gcctcgccaa gggcgcggca
     1141 ctgcacgctc caacggacga agacgctgcc gacagtgaca ccccggccgc tcccggcggc
     1201 gctgaccaac cgccggtacc agcccagcca ggcgaaagtg ccggtgctga cccgtccgcg
     1261 cagccgcccg cgtcatccga ctcggccgaa cagccttctc tgccggcaca acccggtgaa
     1321 agtgtcggtg ccgatccttc tgccggtcct ggcctggtgc cgggcggtgc tgatcagcct
     1381 ccgcgacctg ccacgacggc cgatc
//
LOCUS       DAORHD010001436         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129397_length_1404_cov_4.358784, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001436 DAORHD010000000
VERSION     DAORHD010001436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129397_length_1404_cov_4.358784"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..863
                     /locus_tag="PKO06_22405"
     CDS             6..863
                     /locus_tag="PKO06_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV72478.1"
                     /translation="MILAALLWSTGGVFIKAVDLSPFAISGVRSLFALVTLLVLVGRP
                     RRPTFGAGSHALWLGALFYTLTLFCFVTATKLTTAANAILLQYTAPVYVALMSGFFLG
                     EPIRRSDWTAVIIVMIGMTMFFGDRLDAGGLAGNLLGLISGVFLASMVVSLRSQKDQS
                     THDSIIWGNVLVAIIGTPFLVQGIPSGKGVAALAFLGVFQIGCAYYFYGKAIKKVPAL
                     ESILIPTLEPLLNPVWVWMFIGERPGPWALTGGVIILVTLPVHGLTSAPRKPSEPMVT
                     AEPSSLTTG"
     gene            complement(945..>1404)
                     /locus_tag="PKO06_22410"
     CDS             complement(945..>1404)
                     /locus_tag="PKO06_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72479.1"
                     /translation="EYVRTHRDELLQAAWKEASALLVVKGDSGTSRPGLSGHTVVPKP
                     DSSPKPTGSAAKPSRDEQVQQAIKRFMATEEARAVRNKEDDVKAGKTDTAYKFEWPVP
                     PHVEGDFVVFASVMYQKRDAQSDWFGIGYAGDAQNPCKIAIGSFISQWGK"
BASE COUNT          228 a          424 c          414 g          338 t
ORIGIN      
        1 tgcacctgat tctggcagcc ctgctctgga gcacgggcgg tgtcttcatc aaggccgtcg
       61 atctctcgcc atttgccatt tccggtgtgc gcagcctgtt tgccctggtg acgctgctgg
      121 tgctggtcgg ccggccccgt cggccgacct tcggggccgg ttcacacgcg ctctggcttg
      181 gggcgttgtt ctacacgctc acgctgttct gtttcgtcac cgccaccaag ctcacgacgg
      241 cggccaacgc cattctgctg cagtacaccg cgccggtcta cgtcgcgctc atgagcggct
      301 tcttcctggg cgagccgatc agaagatcag actggaccgc cgtgatcatc gtcatgatcg
      361 gcatgacgat gttcttcggc gatcgactcg atgccggtgg tctggccgga aatctgctcg
      421 gtctgatcag cggcgtgttt ctggcttcga tggtcgtcag cctgcgttcg cagaaagatc
      481 agtcgaccca tgattcgatc atctggggaa atgtcctggt tgcaatcatc ggaacccctt
      541 tcctggtgca aggaataccg agtgggaaag gcgtcgcggc gctcgcgttt ctcggcgtgt
      601 ttcagatcgg ctgcgcatac tacttttatg gcaaggcgat caagaaggtg ccagcgctcg
      661 agtcgattct gattccgact ctcgaaccgc tgttgaaccc ggtctgggtc tggatgttca
      721 ttggcgagcg tccggggccg tgggcgctta cggggggcgt aatcattctg gtcacgctgc
      781 cggtgcatgg cctgacttcc gccccccgca aaccatcaga accaatggtc accgcggaac
      841 cttcttcgct gactaccggt tgatctgccc tcgtcggctg tttccggtgt gctcggttgt
      901 tcccgctgcg gtccgggttg cgattcacga aatccagatc aggtctattt tccccactgt
      961 gaaatgaacg agccgatcgc aattttgcac ggattctgtg cgtcgcccgc gtagccgata
     1021 ccgaaccagt cgctctgggc gtcgcgcttc tgatacatga cactcgcgaa aacgacgaaa
     1081 tccccttcga catgcggcgg aaccggccac tcgaatttat aggccgtatc cgtcttgccg
     1141 gctttgacgt cgtcttcctt gttgcgcacg gctctggctt cctcggtggc catgaagcgc
     1201 ttgatggcct gctgtacctg ctcatcgcga ctgggttttg cagctgaacc tgtgggtttc
     1261 ggcgatgagt ccggctttgg gacaacggta tgaccagaca agcccgggcg tgatgtgccc
     1321 gaatcaccct tcacgacgag cagcgcggaa gcttctttcc aggctgcctg aagcagctca
     1381 tcgcgatgag tgcggacata ctcg
//
LOCUS       DAORHD010001437         1404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129401_length_1404_cov_4.137139, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001437 DAORHD010000000
VERSION     DAORHD010001437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129401_length_1404_cov_4.137139"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..533)
                     /gene="rnhA"
                     /locus_tag="PKO06_22415"
     CDS             complement(54..533)
                     /gene="rnhA"
                     /locus_tag="PKO06_22415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HNV72480.1"
                     /translation="MMTTPSPTPPRKMVQLFCDGACSGNPGPGGWGAILRFGTSEREI
                     SGGAPYTTNNQMELMACISGLQLLKEPCEVLIQTDSQYLAKAFQEGWLEKWQRNGWRT
                     SGKQPVKNRELWEELLKLAAQHQLRWQWIKGHAGHRENERCDELAVKAREQIARGPR"
     gene            complement(530..>1404)
                     /locus_tag="PKO06_22420"
     CDS             complement(530..>1404)
                     /locus_tag="PKO06_22420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72481.1"
                     /translation="EEMTVQLLASQNAQRKAEAEAGIREGTIQVLKTEIARRQKAEED
                     LRAARDRAEAGNIAKSNFLAMVSHELRTPLTAVIAYGKLLADEKGPLGQQTSPDVREF
                     GNRILKSGEHLQGLIDNILSYSELETGQATLKPTLFELESLIEFLEWFVGLQQSRSTH
                     IAFELQRPQQKVQLYSDLMALRQILINVLSNAFKFTHSGKVQFTCAIEDTTLRISVID
                     TGIGIPAAQREQLFQPFFQVSSGKRRRYGGAGVGLAIVKHLVDSLHGSISIDSEEGRG
                     TTVTMTFPDIVRKA"
BASE COUNT          268 a          397 c          403 g          336 t
ORIGIN      
        1 cgaccagaga ttcccgtctg tgaaccgggg gcatcactgc tgttgcatgc tggtcagcgg
       61 gggccacggg cgatctgttc gcgcgccttc accgccagct cgtcacaccg ctcgttttct
      121 cggtgaccgg cgtgtccttt gatccattgc cagcgaagct gatgctgtgc cgcgagcttg
      181 agcagttctt cccagagttc gcgatttttc accggctgct tccctgaggt tctccaaccg
      241 tttcgctgcc atttttcgag ccagccttcc tgaaaggcct tggcgagata ctggctgtca
      301 gtctgaatca gcacttcaca gggctctttc agcagttgaa ggcctgagat gcatgccatc
      361 agctccatct gattattggt cgtatagggg gctccgccac tgatctcccg ttcagaggtc
      421 ccaaaacgga gaatcgctcc ccatccgcca gggcccggat tgccggaaca agctccgtcg
      481 cagaagagct gcaccatttt gcgcggggga gtgggtgaag gcgtcgtcat catgctttgc
      541 ggacgatatc gggaaaggtc atggtcaccg tggtgccccg cccttcctct gaatcaatcg
      601 agatcgaacc gtgtagagaa tcgaccagat gtttgacaat cgccaggccg acccccgccc
      661 cgccatatcg gcgtcgcttg cctgaagaaa cctggaagaa cggttgaaac aactgttcgc
      721 gctgtgctgc cgggatgcca atgccagtat cgatgaccga aatacggagc gtcgtgtctt
      781 cgatggcgca ggtgaattgg actttccctg agtgggtaaa cttgaaggca tttgacagaa
      841 cattgatgag gatctgcctg agagccatca aatcggagta gagctgcacc ttttgctgcg
      901 ggcgctgcag ttcaaaagcg atatgtgtac tgcgagactg ttgcagtccg acaaaccact
      961 cgagaaactc gatcagtgat tccagttcga acagggtggg cttgagcgtg gcctgtccgg
     1021 tctcgagttc agaatagctg agaatgttgt cgatgagacc ctgcaaatgt tctccgctct
     1081 tcagaattcg gtttccgaac tcacgaacgt ccggtgacgt ttgctgaccc agggggcctt
     1141 tctcgtcggc gagcagtttc ccatacgcga tgacagcggt gaggggcgtc cgcagctcgt
     1201 gactcaccat ggcgagaaaa ttcgacttgg cgatgttgcc ggcttctgcc cggtctcgcg
     1261 ccgcgcgcag atcttcttcc gctttctgcc gccgggcgat ttcggtcttg agaacctgga
     1321 tggttccttc gcgaatacct gcctcggctt cggccttgcg ctgggcattt tgcgaagcca
     1381 gaagttgtac ggtcatttcc tcgt
//
LOCUS       DAORHD010001438         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129566_length_1403_cov_3.835312, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001438 DAORHD010000000
VERSION     DAORHD010001438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129566_length_1403_cov_3.835312"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PKO06_22425"
     CDS             complement(<1..1365)
                     /locus_tag="PKO06_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72482.1"
                     /translation="MTKSVYRRALGATTYSLVGSVELGNDKWERFFWVPLTLIGSASY
                     TTINTSNWLGISATPDWHWIDTQPAKDPTPSVGLPTRVQVGVINVAAPPMVFNASGVL
                     DIVGPINDWQTNEVPQGLYTYRVENTPEWERDDFNKKYYNPADPATVTDENSNGTIGG
                     FPSTASKAGTDPSNSRSDHVLYKWVIYQVKDAYGADIPATIVKNSGSVIGIPEEVTNP
                     EIKHYFKAGVYQIMCSARYRYYNYDLLPFGSTVLDKPGVLSAFKNAIVPPKPGMINTS
                     NYPTLAAIPANTVTQMLKVNVQSPIASGTLAADIYRKLPADAAFSAPVVSGVYKYHAI
                     DEIATHVWKMVPENQLFTLPTITDSNKVGAVRWADPVAYYTWSFNLTMPDTSPYPLTL
                     PSKNTSNSADTSVTFGIPIPSDPRLGTLECNAYRTWEYDYRDYDTNGNPLGIKTKSGV
                     ILYRG"
BASE COUNT          277 a          342 c          450 g          334 t
ORIGIN      
        1 gcctcggtac aagatgacgc cggacttggt tttgatgccc agcgggtttc cgttggtatc
       61 gtaatcgcga taatcgtatt cccaggttcg gtaggcgttg cattccaacg ttcccagtct
      121 tggatcagac gggatcggaa tgccgaaggt aaccgaggtg tcggcggagt ttgaggtatt
      181 cttcgagggg agagtcagcg gatacgggct ggtgtcgggc atggtcaggt tgaacgacca
      241 ggtatagtag gcgaccgggt cagcccagcg cacggcacca accttgttgc tgtcggtaat
      301 cgtcggcagg gtgaataact gattctcggg aaccatcttc cagacatgag tcgcgatttc
      361 atcgattgcg tgatacttgt agacgccgga aacaacggga gccgagaaag ccgcgtcagc
      421 cggcagcttg cggtagatgt cggcggccag agtcccagaa gcgatcggcg actggacgtt
      481 caccttgagc atctgcgtga ccgtgttggc tggaatagcc gccagggtgg gatagttgct
      541 ggtgttgatc atgccgggtt ttggcggcac gatcgcgttc ttgaacgctg agagaactcc
      601 gggcttgtcg agaaccgtgc tgccgaacgg cagcagatcg tagttgtaat agcgatagcg
      661 ggcagaacac atgatctgat agacgccggc cttgaagtag tgcttgattt cagggttcgt
      721 aacttcttcc gggattccga tgacggagcc ggagttcttc acgatcgtgg cggggatatc
      781 cgcgccgtag gcatctttga cctgatagat gacccacttg tacaacacgt gatcagaacg
      841 tgaattactc gggtcggtac ctgcttttga agcggtagag gggaatccac cgatagtacc
      901 gttgctgttc tcgtcggtga ccgtggcggg gtcagcgggg ttgtaatatt tcttgttgaa
      961 gtcgtctcgt tcccattcgg gggtgttttc cacccggtag gtgtacaagc cttgcggtac
     1021 ttcgttggtc tgccagtcgt tgatcgggcc gacaatatcc agaacgccgg aggcgttaaa
     1081 caccatgggt ggagcggcga cattgatcac gcctacctgc acgcgagtcg gcaggccgac
     1141 cgacggggtc gggtccttgg ccggctgtgt gtcgatccag tgccagtcag gagtcgctga
     1201 gatacccaac caattcgagg tgttgattgt cgtgtatgag gcgctgccga tgagagtgag
     1261 cggcacccag aagaagcgtt cccatttgtc gtttccgagt tcgacgctgc cgacaagact
     1321 gtaggttgtt gccccgagtg cgcgccgata cacgcttttt gtcactttct gatcataaat
     1381 gaggcgcccg gtggctctat tga
//
LOCUS       DAORHD010001439         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_129580_length_1403_cov_3.248516, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001439 DAORHD010000000
VERSION     DAORHD010001439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129580_length_1403_cov_3.248516"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="PKO06_22430"
     CDS             complement(<1..902)
                     /locus_tag="PKO06_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72483.1"
                     /translation="MRARSSAAYSALSALRHSDSEQSNRLTVSIAKHFTARRRGFLLM
                     EILFAIGLLAILTVYVSGHAGQVGLEADRQKNDMHAVVTGNNMIEIMIATSSTPFDVV
                     TNLLPAPGPDGKIEIAPDSPLWSGGTTTSTVQIVKDADKDNPSSFTVLINNSNSGTQA
                     SYWHVFVPDTALGGTFAANRPPIVLLAATTNLNLVLKGNGKVLSWGRNNFGQAGQGRT
                     GTPAEYDPISSPGLVKGVHGLDSLRDIAFVCAAKNGYTSFAMKSDGTLFGWGKSGIRL
                     FSYQTANQVDVEYPIQIKDHLDRP"
     gene            complement(1087..>1403)
                     /locus_tag="PKO06_22435"
     CDS             complement(1087..>1403)
                     /locus_tag="PKO06_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72484.1"
                     /translation="HVDGERRVCLQRVGKLVLYLAAPGVTSFSYTLQANATFTIDVKY
                     QFTYGDPKHSLLSIATSSVFVDGNRQPSGFEGLKIDATVPLLNLDMPASYSDWHLTTF
                     TP"
BASE COUNT          319 a          362 c          413 g          309 t
ORIGIN      
        1 aacggtcgat cgagatgatc cttgatctgg attggatatt ccacatcgac ctggttcgcc
       61 gtttgatagc tgaacagcct gattcctgac ttcccccatc cgaaaagagt cccatccgac
      121 ttcatggcaa atgaagtgta gccgttcttt gcggcacaga cgaaagcaat gtcacggagg
      181 gaatcaagcc catgaacgcc tttcaccaac cccgggcttg agatcgggtc atactccgct
      241 ggtgttccag tgcgaccttg cccggcttgt ccgaaattgt tgcgacccca cgacaacacc
      301 ttcccatttc ctttgagcac cagattgaga tttgtcgtcg ccgcgagtag tacgataggc
      361 ggccggttgg cggcgaaggt gccgccgagg gctgtgtcgg ggacgaagac gtgccagtaa
      421 ctggcttggg tgccgctgtt ggagttgttg atcaacacag tgaaagagga agggttgtcc
      481 ttatcagcgt cctttacgat ctgcacggtc gaggtggttg tcccacctga ccagagtgga
      541 gaatcaggcg cgatctcgat cttgccgtcg gggccggggg cgggcaggag attggtcacc
      601 acatcaaacg gcgttgacga cgttgcgatc atgatctcga tcatgttgtt gccggtgacg
      661 acggcatgca tgtcgttctt ctggcggtcg gcttcgagcc cgacctggcc ggcgtgaccc
      721 gaaacataca cggtcaaaat ggccaaaagg ccgatggcga acagaatctc catcagcaga
      781 aagccgcgcc gacgggcggt gaaatgcttg gctattgata ccgtcagacg attggattgc
      841 tctgaatctg agtgacgaag tgctgacagc gccgagtaag cggccgatga acgcgccctc
      901 acctgctgac actcatcatg taaggcatcc cgagatgtca tcacctggcc catggctgat
      961 cgagaagcag atgcgatcgg cattgtcagg acattcacaa acgtgttggg ttgcgaagag
     1021 tccgggttcg atcgagtgag aaccttttgt tcaaaaagct tttcaaaggg ttgtttcatg
     1081 aagccctcat ggagtgaacg tcgtgagatg ccagtctgaa tacgaagccg gcatgtctag
     1141 attcagcaat ggaacggtcg cgtcgatctt cagaccttcg aaccccgacg gttgacgatt
     1201 cccatcgacg aagacggatg aggtcgcgat cgagagcagg ctgtgctttg ggtcaccgta
     1261 ggtaaactgg tacttcacgt cgatggtgaa cgtcgcgttg gcctgcaacg tgtacgaaaa
     1321 actggtgacg cctggggcag cgaggtaaag taccagttta cctacacgtt gcaggcaaac
     1381 gcgacgttca ccatcgacgt gaa
//
LOCUS       DAORHD010001440         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130063_length_1400_cov_2.880297, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001440 DAORHD010000000
VERSION     DAORHD010001440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130063_length_1400_cov_2.880297"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PKO06_22440"
     CDS             complement(<1..1011)
                     /locus_tag="PKO06_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72485.1"
                     /translation="MHFDNRRHSLFAAVRSSLASIASHRRGSILFPILGFLLLILPFA
                     LTVLITRRIEREIHRQKYQEARQEVSSIVRRMAARTDPTTHFSELAERIVRAQKWNSS
                     LEALLRGYPPEALEIMRFDAAGKRQGIPGLRLEKQTMTERALTLIRRLSAKTSGTLSK
                     SDERLLDTLLGSHLTAHLLARQPNKLHDLFLFGTRRFSGLYTLGPSHASDGWILIQIH
                     LDPLPRTFYADSTLNSLRATTLPKYQFGIIDLADHTKRHTAFERQLLLDRLSSVFRTG
                     RKLVGLVSLPSGLRLYASREIPAREHPFQVACLFAGAVTGLFLLVCVRLYLVPDLLRS
                     VTL"
BASE COUNT          296 a          380 c          444 g          280 t
ORIGIN      
        1 taaggtcacg gaacgcagca gatcgggaac cagatacaac cgcacacaca ccaacaggaa
       61 gaggccggtg acggcaccgg cgaacaggca ggcaacctga aacggatgtt cgcgggcagg
      121 aatctcacga ctggcgtaga gtctcaagcc gctgggaaga ctcacgagac ctacaagttt
      181 ccgacctgta cgaaacaccg aagacaggcg atcaagcagc agctgtcgct cgaaggcggt
      241 atgacgcttc gtgtggtcgg caagatcgat gatgccgaac tgatacttcg gcaacgtcgt
      301 tgcccgcaga gaattcagcg tcgagtcggc atagaaagtt ctcggaagag ggtcaaggtg
      361 aatttgaatg agaatccagc cgtcagacgc gtgagaggga cccagcgtat acagacctga
      421 aaaacgtcga gtgccgaaca gaaacaggtc atgcagcttg ttgggctggc gtgccagaag
      481 gtgagcggta agatgacttc ccagcagcgt gtcgagcagg cgctcgtcgc tcttcgacaa
      541 cgttccggac gttttggcgc tgagacggcg tatcagggtc agtgctcgtt cggtcatcgt
      601 ctgcttttcg aggcgcagcc caggaatgcc ctgtctcttt cctgcggcat cgaaacgcat
      661 gatttcaagt gcttcaggtg gatagccccg caacaacgcc tcgagcgacg agttccactt
      721 ctgggcacga acgatccgtt ccgcaagttc agaaaagtgc gttgtcggat ccgttcgcgc
      781 tgccatgcga cggacaatcg aactgacttc ctggcgcgcc tcctggtact tctgccggtg
      841 aatctctcgc tctatccgtc tggtgatcag aaccgtcagg gcgaacggca gaatcagcag
      901 caggaacccc aggatgggga acaggatact gcctcggcgg tggctcgcga tcgacgcgag
      961 actgcttctg acagctgcga acagagagtg ccgtcgattg tcgaaatgca ttcgtctacc
     1021 tgaacgtgcg tcgctctgca tctctgatcc gagttcagag cggttcttct attccgttaa
     1081 tgattctctc tgcctaccgc accgctcagg accgtgctgg tccactctcc tgcgctgtac
     1141 ccggcttaaa acggaatatc gattacggcg tgctggcgtc atctagcgtg ggccgggtgg
     1201 gcgatgcggt ggcggttgat gaccaccctg cggcgggggt gggcggtgcg gcggcggaaa
     1261 gggcggggga ggtcggcgca tgtcgcccgg agaggttccg ggtggcgtgc gcgtcgtgga
     1321 agcgagttct gatccagggt ttacgggctg cggcggtttc cctcctccgg gtgggatctt
     1381 gaatcctttg aaatacaggc
//
LOCUS       DAORHD010001441         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130375_length_1398_cov_2.802681, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001441 DAORHD010000000
VERSION     DAORHD010001441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130375_length_1398_cov_2.802681"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="PKO06_22445"
     CDS             <1..717
                     /locus_tag="PKO06_22445"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV72486.1"
                     /translation="RLPVTTTLYRTWEETLPAIENSLRERTQTYYICPRIERTEKSDW
                     GAVRDAETKLQQALPRARLASLTGQDTAEAREATLRAFGAGEIDLLVATTIVEVGMDF
                     PNATLLIVENAERFGMSQLHQLRGRIGRGSKGGTCLIVSDAADENERLQIFCSTSDGF
                     AIAAADLRFRGPGDLVGTSQSGLNHPALAHLDNPDRILQARERARQILQQEPEPVRNW
                     FLEKMQASFGDAWQKFMEGG"
     gene            719..1306
                     /gene="rsmD"
                     /locus_tag="PKO06_22450"
     CDS             719..1306
                     /gene="rsmD"
                     /locus_tag="PKO06_22450"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HNV72487.1"
                     /translation="MRIIGGSAKGRILKMPGGGKTRPAMSRVRGSYFNIIAPRIEESR
                     FLDLFAGSGSVGIEALSRGAAFAVFVDKSPLCTRTIRENLEMTRFLEQSQVFQEDCVG
                     FLKRYTADPFDFVAIDPPYLKGLLDPVLELIPDCSLFHEKTLFMIERQKKDDLHFEKR
                     SRLILTDERNFGDTVLTFFRKRIVEAPPPETPPTA"
BASE COUNT          298 a          385 c          412 g          303 t
ORIGIN      
        1 cggcttcctg tgaccaccac cctgtaccgc acctgggaag aaaccctgcc ggccatcgaa
       61 aacagcctgc gcgaacgaac gcagacgtat tacatctgcc cgcgcatcga acgtaccgag
      121 aaatcagact ggggtgcggt tcgcgatgcc gagacgaaac tgcagcaggc gttgcctcgg
      181 gctcgtcttg cctcgctgac cggtcaggat acagcggaag cgcgcgaagc cacgttgcgg
      241 gcatttggcg cgggggagat cgatctgctc gtcgccacga ccattgtgga agtcggcatg
      301 gattttccca acgcgacgct cttgatcgtc gaaaatgctg agcgctttgg tatgtcccag
      361 ttacaccaac tgcgcgggcg catcgggaga ggaagcaagg gtggaacctg tctgattgtc
      421 agtgacgcgg ccgacgagaa cgaacgattg cagatcttct gctcgaccag cgacggattc
      481 gcgatcgccg cagccgacct gcgctttcgc ggaccgggtg atctggtggg taccagtcag
      541 agcggtctga atcacccggc tctcgcgcat ctcgacaatc ctgatcgaat tctgcaggca
      601 cgcgaacggg cgcggcagat tttgcagcag gaacccgagc cggtgcgaaa ctggtttctc
      661 gaaaagatgc aggcaagctt cggcgatgcc tggcagaaat tcatggaagg aggctgacat
      721 gcgcatcatc ggcggatcag ccaaaggaag aatactcaag atgccaggcg gcggcaagac
      781 gcgcccggcc atgagccggg tgcgtggttc gtatttcaat atcatcgcgc ctcgcatcga
      841 ggagtcgcga tttctcgatc tctttgcagg gtcaggcagt gtcgggatcg aagcgctctc
      901 tcgcggtgct gcctttgccg tctttgtcga taaatcaccg ctgtgcaccc ggacgattcg
      961 tgagaatctc gaaatgacca gatttctcga acagtcgcag gtttttcagg aagattgcgt
     1021 cgggtttctc aagcggtaca ccgctgaccc gttcgacttc gtggctatcg accccccgta
     1081 tctgaaaggc ctgctggacc cggttctcga actgattccc gattgttcgt tgtttcatga
     1141 gaagacgttg ttcatgatcg agcgtcagaa aaaggacgat ctgcattttg aaaaacgatc
     1201 gcgattgatt ctgaccgacg aacgcaattt cggagacacg gttctgactt tttttcgcaa
     1261 acgaattgtt gaagcgccgc cgcctgagac gccacccacg gcttgagtgt agctccggtt
     1321 cacgttttcc gggcatgaaa aaaacccagc cttgcggctg ggtttttttt atctgccggg
     1381 gggattactt gataccgg
//
LOCUS       DAORHD010001442         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130433_length_1398_cov_2.137007, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001442 DAORHD010000000
VERSION     DAORHD010001442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130433_length_1398_cov_2.137007"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PKO06_22455"
     CDS             complement(<1..863)
                     /locus_tag="PKO06_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72488.1"
                     /translation="MKVWLVILLLLGSVPVQACDDTLLALLTASDPNSEFSVSIRKLN
                     AQIAALGAVLNESPDQDVSSLVEQLMTAWLEFSNRYNVNPPEIAREDPDWTGKMAEAA
                     KRIGRISLLIDQKNRFQAHLECLQLSGYLARFFDIVTMSPLKRSFLKAAEKYIALDKA
                     CQMKDVPTLQAGLVDLETFLATYTPMLATATRASFEKTLTALRDVRGTLASTTDAAWA
                     DATQLKIDAARNQFQQFRARLLMNEWFPNIKSVPVEPLTPPLPEMKLVEPDSGAVPAT
                     DSAPTPPVATAS"
     gene            complement(860..>1398)
                     /locus_tag="PKO06_22460"
     CDS             complement(860..>1398)
                     /locus_tag="PKO06_22460"
                     /inference="COORDINATES: protein motif:HMM:NF020318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HNV72489.1"
                     /translation="YRSDLACVLIDTSFSAILGCTFDPRVAALRGPVLYINFGNGHTM
                     ACILDSSGPTGKSTAGSDSLPETVRLLSFFEHHTRILKQKPECLQDYVRRLAEGRLLS
                     DEVFDDDGNGCVTFEKIRFEQLAGVVVTGPQRWLMPRTGIGSYIEAAPGGDMMMTGPL
                     GLLHGMALRPQDFRGVTT"
BASE COUNT          313 a          401 c          401 g          283 t
ORIGIN      
        1 gatgcggtgg caactggcgg ggttggcgcg gagtcggtgg caggcaccgc gccagaatcc
       61 ggctcaacca gtttcatctc gggaagcggt ggtgtgagcg gctcaaccgg cactgacttg
      121 atgttgggaa accactcgtt catcaacagc cgcgcccgaa actgctgaaa ctgatttcgt
      181 gccgcatcga tcttcagctg cgtggcatcc gcccaggcgg catcggtagt tgacgcgaga
      241 gtccctctga catccctcag agccgtgagc gttttttcga aactcgctct cgtcgcggtc
      301 gccagcattg gcgtgtaggt ggcaagaaag gtctcaagat caacaagacc cgcctgcagt
      361 gttggaacat ctttcatctg gcaggctttg tcgagcgcga tgtacttctc tgctgctttg
      421 agaaaggagc gtttgagcgg cgacatggtg acaatatcga aaaaacgtgc cagatatccg
      481 ctcagttgca gacactccag gtgtgcctga aaccggttct tctgatcgat aagcagagaa
      541 atcctgccga tgcgcttggc ggcttcggcc atttttcccg tccagtccgg gtcttcacgg
      601 gcgatctcgg gtgggttgac attgtaccgg ttggaaaact ccaaccaggc cgtcatcagc
      661 tgttccacaa gagaactgac atcctggtct ggcgactcgt tgagcacggc accaagcgcg
      721 gcgatctgcg cgttcagttt gcgaatcgac acactgaact cactgttcgg gtcactggct
      781 gtcaacagcg ccaacagcgt gtcatcgcac gcttgaaccg ggacagaacc gagaaggaga
      841 agaatcacca gccaaacctt catgtggtca ctcctcgaaa atcctgcggt cgcagagcca
      901 tgccatgcaa caatccaagc ggaccggtca tcatcatgtc accaccgggg gcggcctcga
      961 tataggaccc gataccggtt ctcggcatta accagcgctg cgggcccgtc accacaaccc
     1021 cggcgagctg ttcgaaacga atcttctcga acgtgacgca gccgttccca tcgtcatcaa
     1081 acacctcgtc gctgagcagg cggccttccg ccaggcgtcg cacatagtcc tgcaggcatt
     1141 ccggtttctg tttgagaatc cgggtatgat gctcaaagaa tgacagcagg cgcactgttt
     1201 cggggagcga gtcggaacca gcggtcgatt tgccggtcgg gcccgatgaa tcgaggatac
     1261 aggccatggt gtgaccattg ccgaagttga tgtaaagcac cggcccgcga agcgccgcga
     1321 ctctcggatc gaatgtacaa cccagaatcg ctgaaaaaga cgtatcgatg agcacgcagg
     1381 caagatcgga gcgataac
//
LOCUS       DAORHD010001443         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130562_length_1397_cov_2.540984, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001443 DAORHD010000000
VERSION     DAORHD010001443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130562_length_1397_cov_2.540984"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="amrB"
                     /locus_tag="PKO06_22465"
     CDS             complement(<1..537)
                     /gene="amrB"
                     /locus_tag="PKO06_22465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV72490.1"
                     /translation="MKYACVLLMMGLVSSFASPSRVWAGEGPWGLRAPAVAGQFYPGT
                     ADELKAAVSSYLTKAKPPFSCASESRVVALIVPHAGYVFSGATAGLGFRALENVSFDR
                     YCFLGVDHRSGLSTISVWPDGGFDTPLGPAPVDGSFTRRLLENGPPVTAESSQHVAEH
                     SIEVLVPFFQVVFEGKSAT"
     gene            complement(627..>1397)
                     /gene="dapF"
                     /locus_tag="PKO06_22470"
     CDS             complement(627..>1397)
                     /gene="dapF"
                     /locus_tag="PKO06_22470"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV72491.1"
                     /translation="DVRFGVGADGLVFIMSPHDNRHQCRMQVFNADGSEAEMCGNAIR
                     GVAHWYKKHHLGFEPILVETRSGVRQVYSSDVHDGVCMYRVEMGKVNFDLTTSGELLP
                     ESERKPLVWDEETLAPQYANVGNPHAVLFLNQPLSQDALIKMGAWLETHPNHPRRMNI
                     EFVEILSPHEVKATVWERGCGMTHACGTGATAIAATGIKNGKLQSPVTVHMPGGDLIL
                     EQMGDGTMFMTGPVQEVCDGHLAPSFVNVMLNHPPAIK"
BASE COUNT          276 a          432 c          433 g          256 t
ORIGIN      
        1 ggtggcgctc ttgccctcga acaccacctg aaagaaaggc accaggacct cgatcgagtg
       61 ttccgccacg tgttgcgacg actcggccgt gacgggaggc ccgttctcga gcagccgtcg
      121 ggtgaaactg ccgtcgacgg gtgcgggccc gagcggggtg tcgaacccgc cgtcaggcca
      181 gaccgagatc gtcgacagcc cggaacggtg atcgacgccg agaaagcagt accggtcgaa
      241 cgacacgttc tcgagggcgc gaaagcccag acctgccgtc gccccggaaa aaacataccc
      301 ggcatgcgga acgatcagcg cgacgacacg agattcagaa gcgcatgaga agggtggttt
      361 ggccttggtc agataggacg aaaccgccgc tttcagttcg tccgcggtgc ctgggtaaaa
      421 ctgccccgcc acggccggag cacgaagacc ccacggcccc tcgccggccc agactcgtga
      481 cggactcgca aacgacgaga caagacccat catcaacaaa acgcatgcgt atttcaccgc
      541 gtgtactccg gtttcccgat gattcttcga ttgtccgagt ccgtgaacga caacgcgaag
      601 gtgagaagcc tatccgaaga tgtccgttac ttgattgccg gggggtgatt cagcatgacg
      661 ttgacgaagc tcggggcgag atgtccgtca cagacttcct gaaccgggcc ggtcatgaac
      721 atcgtgccgt cgcccatctg ttcgagaatc agatcgccgc ccggcatgtg caccgtgacc
      781 ggcgactgca gttttccgtt cttgatcccg gtggcggcga tcgcggtcgc tccggtgccg
      841 caggcgtgtg tcatgccgca cccgcgctcc cagaccgtcg ctttgacctc gtgcggcgag
      901 agaatctcga cgaactcgat gttcatgcga cgcggatggt tggggtgggt ttcgagccag
      961 gcccccatct ttattagggc atcctgcgag aggggttgat tgagaaacag tacggcatgc
     1021 gggttgccga cgttggcata ctgcggcgcg agggtctctt catcccagac caggggcttg
     1081 cgctcgctct cgggcaagag ctcgccgctg gtggtcagat cgaagttgac cttgcccatc
     1141 tcgacgcgat acatgcagac accatcgtga acgtcgctcg agtagacctg ccgcacgccg
     1201 ctgcgcgttt cgaccagaat cggctcgaat ccgaggtggt gctttttgta ccagtgcgcg
     1261 accccgcgaa tagcgttccc gcacatctcg gcttcgctgc cgtcggcgtt gaagacctgc
     1321 atgcggcact ggtggcgatt gtcatgcggc gacatgatga aaaccagccc gtctgcgccg
     1381 accccgaagc gaacatc
//
LOCUS       DAORHD010001444         1397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130574_length_1397_cov_2.277943, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001444 DAORHD010000000
VERSION     DAORHD010001444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130574_length_1397_cov_2.277943"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..>1397)
                     /locus_tag="PKO06_22475"
     CDS             complement(193..>1397)
                     /locus_tag="PKO06_22475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV72492.1"
                     /translation="QLLDYQWGMFWRSCELFQIPRNRWIIQMPTGSGKTRTAMELIHT
                     VLDAPENHNAIILWLAHSEELCEQAIDCFVSTRQHVGRFPSYVVRMWGNQHRGPLPQG
                     RTFVVITYQKLVAMYRSEQNTEDFRRSRLVILDEAHMAVAPQYNKAIQNLLREGTSTL
                     VGLTATPIRTIESETLKLREFFLDNLVAITDGGKNDLNDLLKRGILSHPTFELIETEQ
                     SFELTTKEISDLENQFDFPPGLLKRIGSHDLRNGMILRRLQELVAEGRSILLFACSVD
                     HSRFLASTLEFLGVRAHHLDGTMDRGARSSAISDFRHKKVRVLCNYGVLTTGFDAPNT
                     DVVFIARPTSSPVLYSQMVGRGMRGPRMGGNTSFRVIDVRDNFFDHGQPLDLFARFQA
                     KWQKCFVV"
BASE COUNT          374 a          324 c          353 g          346 t
ORIGIN      
        1 tcgcttttga tgaacacatc aagaacctgc cagaatttga tttttcaggc ttcgggccgc
       61 caagcgagga agtcatgatg gcacttgaga accgaaaaga gttcccaatg gttttcaaga
      121 atgtcggggc atgaactcaa aagaggcgca cagcgcagac taggttttat cggtggacgg
      181 caacatgttc gctcagacaa caaaacactt ttgccacttt gcctgaaagc gtgcgaagag
      241 atcaagcggc tgaccatgat cgaagaaatt gtcccggaca tcgatgactc ggaatgatgt
      301 gttacccccc attcgagggc cccgcatgcc tcgtccgacc atctgagaat acaaaacagg
      361 tgagctcgtc gggcgagcga tgaagacaac atcagtattt ggtgcatcaa aacctgtggt
      421 tagtacccca tagttgcaga gtacgcgaac cttcttgtgt cgaaagtctg agattgccga
      481 actgcgtgct ccgcggtcca tcgttccatc aagatggtga gcgcgaacgc caagaaactc
      541 gagtgtcgat gcgagaaacc gagaatgatc gacactacac gcaaacaaga ggattgaacg
      601 accttcggca acaagttctt gaaggcgcct gagaatcatc ccatttcgca gatcatgact
      661 accgattctt ttcagcagac cgggcggaaa gtcgaattga ttctcaagat cagaaatttc
      721 ttttgtggtt aactcaaatg attgttcagt ctcgatcagt tcgaatgtag ggtgagagag
      781 aattccacgt tttagcaagt cgttcagatc atttttccca ccatcggtta ttgcgacaag
      841 attgtcgaga aagaattctc gaagtttcag ggtttcactc tcgattgtcc gaatcggtgt
      901 tgccgttaaa ccgacgagtg tgctggtacc ttcacgtaga aggttttgaa tagctttatt
      961 gtactggggt gcaacagcca tgtgagcttc atcaaggata accagtcggc ttctgcgaaa
     1021 atcttcagtg ttctgctcac ttcgatacat ggctacaagc ttctggtagg tgatgacgac
     1081 aaaggtgcgc ccttgtggaa gcgggccgcg atgctggttt ccccacattc gaaccacgta
     1141 gcttgggaaa cggccaacat gctgacgagt agagacgaag caatcaatag cttgttcgca
     1201 tagttcctcg ctgtgcgcta gccagagaat gatggcatta tggttttccg gtgcatcaag
     1261 aacggtatga ataagttcca tcgcggtgcg agtcttgcct gaccccgtcg gcatttgaat
     1321 gatccaacga tttcgcggaa tctgaaatag ttcacaagaa cgccaaaaca tgccccattg
     1381 atagtctaag agttgtt
//
LOCUS       DAORHD010001445         1395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130816_length_1395_cov_3.634328, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001445 DAORHD010000000
VERSION     DAORHD010001445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130816_length_1395_cov_3.634328"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PKO06_22480"
     CDS             <1..1084
                     /locus_tag="PKO06_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HNV72493.1"
                     /translation="TIDLLLDKNRVVVCQVAPAVRVAMGELFGIDEDNVAPRLVDGLH
                     RIGFKHVFDTNFAADLTIMEEGTELVGRVTNKGVLPMITSCSPGWIKFIEHFYPNLLP
                     NLSSCKSPQQMQGAMIKHYWAKKMGIDPAKVAVVSIMPCTAKKFEAQRPEMEVDGIRD
                     VDVVLTTREVAKLFQNFDLDLKVCEPKEFDNPLGESTGAGAIFGATGGVMEAALRTAY
                     KLITGNELEKLDVEAVRGFDGVKEAVVKVGNLDVSIAVASGLQNAHTLLKMVMSGEKK
                     YHFIEIMGCPGGCLNGGGQPTTFDADVLKKRLAKIYSIDKRAKMRRSHENPSIQQVYK
                     EFLEKPNSHTAHKFLHTHYHERSEEI"
     gene            1146..>1395
                     /locus_tag="PKO06_22485"
     CDS             1146..>1395
                     /locus_tag="PKO06_22485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV72494.1"
                     /translation="MGVVKHVGGRCKRCYTCVRNCPVKAICVEGGEATVIEDLCISCG
                     TCVRVCSQKAKQAEAHIDRVFQAFATGTEVVALIAPSFD"
BASE COUNT          315 a          402 c          409 g          269 t
ORIGIN      
        1 caccatcgat ctgctgctcg acaagaaccg cgtggtggtc tgccaggtgg cccccgccgt
       61 gcgggtcgcc atgggcgaac tgttcggcat cgatgaagac aacgtggctc cccgactggt
      121 cgacggtctg catcgcatcg gcttcaagca cgtgttcgat accaacttcg ctgccgatct
      181 gacgatcatg gaagaaggga ccgaactggt cgggcgcgtg accaacaagg gcgtgttacc
      241 gatgatcacc tcgtgcagcc ccggctggat caaattcatc gaacacttct accccaacct
      301 gctgcccaac ctgtcatcgt gcaagtcgcc acagcagatg cagggcgcga tgatcaaaca
      361 ctactgggcc aagaagatgg gtatcgaccc ggcaaaggtg gcggtggttt cgatcatgcc
      421 ctgtaccgcc aagaaattcg aagcccagcg accagagatg gaagtcgacg ggattcgcga
      481 tgtcgacgtc gtgctgacca cgcgcgaagt cgccaagctg tttcagaatt tcgacctcga
      541 tctcaaggtc tgcgaaccga aggaattcga taacccgctc ggtgagtcga ccggtgccgg
      601 cgctatcttc ggcgcgaccg gcggtgtcat ggaagcggct ctgcgcacag cgtacaagtt
      661 gatcaccggc aatgagctcg agaagcttga tgtcgaagcc gttcgcggat tcgacggggt
      721 caaggaagcc gtggtcaaag tcggtaacct cgatgtctcg atcgccgttg cgtcggggtt
      781 gcagaatgcc cacaccctgc tgaaaatggt catgagcggc gaaaagaagt atcacttcat
      841 tgagatcatg ggctgccccg gtggatgtct caatggtggc ggtcagccga cgacgttcga
      901 tgccgatgtg ctcaagaaac gcctggccaa gatctattcg atcgacaaac gggccaaaat
      961 gcgccgatct cacgagaatc cgtcgattca gcaggtctac aaggagttcc tcgaaaagcc
     1021 gaactcgcat acggcccaca agttcctgca cacgcattat cacgagcgca gcgaagagat
     1081 ctgaccgtac cggttgccct cagcgcaatc gctgagggca accagcattt acaggagcag
     1141 acatcatggg cgtggttaaa catgtcggtg gccggtgcaa gcgctgctac acctgcgtgc
     1201 gcaactgtcc ggtgaaagcc atctgcgtcg agggcggaga agcgaccgtc atcgaagatc
     1261 tctgtatctc ctgtggcacc tgcgtgcggg tctgcagcca gaaagccaaa caggccgaag
     1321 cccacatcga tcgcgtcttt caggcatttg cgactgggac tgaagtcgtg gccctgattg
     1381 ccccctcgtt tgatc
//
LOCUS       DAORHD010001446         1394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_130989_length_1394_cov_3.497386, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001446 DAORHD010000000
VERSION     DAORHD010001446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130989_length_1394_cov_3.497386"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PKO06_22490"
     CDS             complement(<1..629)
                     /locus_tag="PKO06_22490"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HNV72495.1"
                     /translation="MSWLLSIGVCFPLWAGPFTFTVSDAVALGDGETIVATLSWSVEP
                     DHYLYRDRMAVKAGNSPVQVSWPTPKEKPDPFDGSLVQMYPVGVHEAGFRLVPMAAQR
                     EIGLRVDYQGCSTTTCFMPTHYTASITVPVAVTPKEPAVKIVSVSESKRDAHATEDAP
                     GGVSTPSGETDFAKVLREQGMLWAMALAFLGGLLVSLTPCVYPMIPITLS"
     gene            complement(698..898)
                     /locus_tag="PKO06_22495"
     CDS             complement(698..898)
                     /locus_tag="PKO06_22495"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HNV72496.1"
                     /translation="MKISWSIPKMKCDGCVQAIAETLLMMEALKEVTVDLSAKKVEFE
                     ATDAASAEEAKRALVRAGFPPA"
     gene            complement(924..>1394)
                     /locus_tag="PKO06_22500"
     CDS             complement(924..>1394)
                     /locus_tag="PKO06_22500"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNV72497.1"
                     /translation="IRDGKVVNRATALNDFVIVKDTISRVIDVSVKISGTHISSYRGD
                     GVIFSSSTGSTAYSLSAGGPIVPPWVNLMIVCPLASHTLNARPVVTSDREILLAELSC
                     SHAQVNLVTDGQEGFPLLSGDQVEVSRAPYTARIVGLKSRNFFKVLRRKMKWGK"
BASE COUNT          329 a          428 c          363 g          274 t
ORIGIN      
        1 gagagagtga tcggaatcat cggatacacg cagggagtca ggctgaccag caggccgccc
       61 agaaatgcca gtgccatggc ccagagcatt ccctgctccc gcagcacctt cgcgaagtcc
      121 gtctcgccgg acggggtgga aacgcccccc ggtgcgtcct cagtcgcatg cgcgtcacgt
      181 tttgattccg ataccgatac tatcttgacg gcaggctcct ttggcgtcac tgcaacaggg
      241 actgttatcg atgccgtata gtgagtcggc atgaaacagg ttgtggtcga acacccctga
      301 taatccaccc gaagaccgat ttcacgctgg gccgccatgg gtaccaggcg aaagcctgct
      361 tcatgcacac cgaccgggta catctgcacc agactaccgt caaagggatc cggtttttct
      421 ttcggagtcg gccaggaaac ctgaaccgga gaattgcctg ctttcacggc catccgatca
      481 cgatacagat agtggtcagg ctcaacagac caactcaacg tggcgacgat cgtttcacca
      541 tctcccaaag caactgcgtc cgagaccgta aacgtgaatg gcccggccca gagaggaaaa
      601 cagacaccga tactcagcag ccagctcacg atcaaaccgg ctcgccacgc gattctgcca
      661 acgaatctac cagtcatgtg cgaatccgtt tccgatatca ggccggcgga aaaccggctc
      721 gcaccagtgc tcgcttggct tcctcagcag acgcggcatc ggtggcctcg aattcgactt
      781 tcttcgccga gaggtctacc gtcacctctt tcagtgcttc catcatcaac agcgtttcag
      841 caattgcctg cacacatccg tcgcatttca ttttcggaat actccaggaa atcttcatcg
      901 tcaccaacct cgcttcgaga gtttcacttt ccccacttca tcttgcggcg cagcactttg
      961 aagaagttgc gcgatttgag cccgacaatg cgagcggtgt atggcgcgcg cgatacttcc
     1021 acctgatcgc cactcagcaa cgggaaccct tcctgaccat cagtcacaag attgacctgg
     1081 gcatgcgagc aggaaagttc ggccagaagg atctcgcgat cggaagtcac caccggccgg
     1141 gcattcagcg tgtgcgaagc caagggacag acgatcatga gattcaccca gggcggcacg
     1201 atcggcccgc ctgccgacag cgagtaggcg gtcgaaccgg tcgaggaaga aaagatcacg
     1261 ccatcgccac gataggaact gatgtgcgta ccagagatct tcaccgagac atcgatgaca
     1321 cgtgagatcg tatctttcac aatgacaaag tcattcagag cggtcgcgcg gttcacgacc
     1381 ttcccgtcgc gaat
//
LOCUS       DAORHD010001447         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131120_length_1393_cov_3.252616, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001447 DAORHD010000000
VERSION     DAORHD010001447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131120_length_1393_cov_3.252616"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1186
                     /locus_tag="PKO06_22505"
     CDS             89..1186
                     /locus_tag="PKO06_22505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV72498.1"
                     /translation="MARKGYILLLVILGAISLGFFGLTLTSLNRGYRSQVVHTRQMQT
                     SFQIGYSGYQRILARLYLKPWEERFFAGSPKAEAGFALYGGTYDTYCIDAPGNPNQAD
                     IYIRVKLEGMTRTYVWRIEHVPSLLDARYVRTILFGEIENADYPSGSAAGYSQKINDL
                     MQTRETNRPKADDLRTKVNGVTTLEDVTSLLNAPRPVLPASHALPIDHPPAEAPAISD
                     PGPLTEAGPGNLIREPGGDTSTPKADELSEKMKANGKIISRILFYPERYDILPESYPI
                     LESVKKMLENMPHIRVSVEGHIALGHDSDSAQVEFSRRRAEVVKQWLVDHGIAASRLV
                     TKGWGDQKPIYENDTATHKKRNRRVEFVRIP"
BASE COUNT          339 a          370 c          403 g          281 t
ORIGIN      
        1 catcttaggt taggcgaatc ttagaaaaca aattaaagga gagtaataaa atgaagagag
       61 gaaaaacaga atgggagagt gccgagttat ggcaaggaaa gggtacattc ttctgctggt
      121 cattctgggc gcgatcagcc tcgggttctt cggcctaacc ctgacctcac tgaaccgggg
      181 gtatcgatct caggtcgtgc acacgcgaca gatgcagacc tcatttcaga tcggatactc
      241 cggctatcag cgcattctcg cccgtctgta tctgaaaccc tgggaagaac gcttttttgc
      301 cggctcgcca aaggctgaag ccggatttgc tctgtacggt ggcacctatg atacctattg
      361 tatcgatgca ccagggaatc cgaatcaggc ggatatctac attcgcgtga agcttgaagg
      421 catgaccaga acgtatgtct ggcgcatcga acacgtgccg tcgttgctcg atgcgcggta
      481 tgtgcgcacc atcctgttcg gtgaaatcga gaatgcggat tatccgtctg gcagcgcggc
      541 aggatatagt cagaagatca atgatctgat gcagactcgc gagacgaacc gcccgaaggc
      601 ggatgacctg cgaacgaagg tgaacggggt gacgacgctc gaagatgtca cgagcctcct
      661 aaacgcgcca agaccggtgt tgccggcaag tcacgcatta cccatcgacc acccgccggc
      721 tgaagcccct gcgatcagcg atccggggcc gctcacagaa gccggcccgg ggaacctgat
      781 cagggaaccg ggcggcgaca ccagtacgcc gaaagcggac gaactgtccg agaagatgaa
      841 ggccaacggc aagatcattt ccagaattct tttctatcct gagcgctacg atatccttcc
      901 ggagtcgtat ccgattctcg aaagtgtgaa gaagatgctc gaaaacatgc cgcacattcg
      961 ggtgtcagtc gagggacata tcgcccttgg tcatgatagc gattccgcac aggtcgagtt
     1021 ttcacgccgc cgtgccgaag tggtcaaaca gtggctggtc gatcacggca tcgcggcctc
     1081 gcgcctggtg acgaaaggct ggggcgacca gaagccgatc tatgagaacg acaccgcgac
     1141 gcacaagaaa cggaatcggc gcgtcgagtt tgtccgtatt ccatgagtgc agaacgattt
     1201 ctgcgagcgg atgacagagc gctgaaaacc tgattacgcg tctctgcttc agctgccgga
     1261 aaccgccgca tactgagaga tgattccctg aatcgtgggt gctgcttgag tcaccacgag
     1321 ctgaatgcgt tgcagaaagc cgtgctgttc agcgagcgtg gctgccgatt gagcccagtg
     1381 cttctcttgc acc
//
LOCUS       DAORHD010001448         1393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131129_length_1393_cov_2.834828, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001448 DAORHD010000000
VERSION     DAORHD010001448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131129_length_1393_cov_2.834828"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKO06_22510"
     CDS             <1..498
                     /locus_tag="PKO06_22510"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV72499.1"
                     /translation="LFAEPLPSMTLTETNGKYRLLSGKELDDETRPAWAPIEAHIKRF
                     LAADIIVISTPMWNFGMPYVLKHYIDVIVQPRYLFKYTENGPVGLAGGRKVIVVTTRG
                     GDYSEGPAKAFDQLEPSLRVVLGFIGISDPIFINAQPMLGSGPEIAAVRIQEGRAVAG
                     NLSLA"
     gene            complement(542..>1393)
                     /locus_tag="PKO06_22515"
     CDS             complement(542..>1393)
                     /locus_tag="PKO06_22515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV72500.1"
                     /translation="LGFKLSEEEAAIRYYREMAAPHLIPFPTREQPKAADPLPEGLEV
                     WDIGSPLDEVDWQGSTMLNPAIIPGFTTMRRVMGTSEGELPEKLPIDLDLYVDSSGSM
                     PDPRHQISYLTLAGTIIALSALRVGARVQATLWSGKREFLKTDGFVRDGTEIMKVLVG
                     FFGGATAFPVHILRDTYLDRKPNDRKVHIMVISDDGVDTMLDNDEKGNSGAVIAAEAL
                     KKGGAGGSLVLNLYGGTIRNPKIKKLGEQGWNLYPISDWPSLTQFARDFSRAVYAAES
                     GSKLLKR"
BASE COUNT          284 a          423 c          398 g          288 t
ORIGIN      
        1 ctcttcgccg aaccgctgcc ttccatgacg ctcaccgaga cgaacggcaa gtaccgactg
       61 ttgagcggca aggagctcga tgacgaaacc cgccccgcct gggcacccat tgaagcgcat
      121 atcaagcgct ttctcgccgc tgacatcatc gtcatcagca ccccgatgtg gaacttcggg
      181 atgccttacg tgctcaagca ctacatcgat gtcatcgtgc agccgcgcta tctcttcaaa
      241 tatacggaaa acggcccggt cgggctggca ggcggcagga aggtgatcgt cgtgacaact
      301 cgcggcgggg actacagcga gggcccggcg aaagccttcg accagctcga acccagtttg
      361 cgcgtcgttc tcggcttcat cggtatctca gacccgatct tcatcaacgc ccagccgatg
      421 ctcggttccg gccccgagat cgccgccgta aggattcagg aaggtcgcgc agtggcgggg
      481 aatctctcac tggcatgact ccggtttcct ctgctgatcc ggctctcaga atccgtattc
      541 ttcagcgttt gagcaacttg gagccggatt cggcggcgta gacggcgcgg ctgaaatcgc
      601 gggcaaattg ggtgagggaa ggccaatcag agatcgggta gagattccag ccctgctcac
      661 cgagcttctt gattttgggg ttgcgaatag tgcccccata caggttgagc accagactgc
      721 cgccggcgcc gccctttttc agcgcttcag ccgcaatcac cgccccagag tttcccttct
      781 cgtcattgtc gagcatggta tccactccgt cgtccgagat gaccatgata tgcaccttgc
      841 gatcgttggg ctttctgtcc aggtaggtgt cgcgtagaat atggacagga aaagcggttg
      901 cgccaccgaa gaatccgacg agaaccttca tgatctctgt cccgtctcgc acgaatccgt
      961 cggtcttgag aaactcgcgt ttgccgctcc acagcgtggc ctgcactctt gcgccgactc
     1021 gcagggccga gagcgcgata atcgtgccgg ccagggtgag atacgagatt tgatgacgag
     1081 ggtcgggcat cgatcccgaa gaatcaacgt acaagtcgag atcaatcggc agcttctcgg
     1141 gaagctcgcc ttcactcgta cccataacgc gacgcatggt ggtgaaacct gggatgatcg
     1201 ccgggttcag catcgtcgat ccttgccagt cgacctcatc gagcggagag ccgatatccc
     1261 agacctcaag cccctcgggc aagggatcgg cggccttcgg ctgttccctg gtcggaaatg
     1321 gaatcaggtg gggagccgcc atctcgcggt agtaacgaat cgccgcttcc tcttcactca
     1381 gtttgaaacc aag
//
LOCUS       DAORHD010001449         1391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131446_length_1391_cov_2.964072, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001449 DAORHD010000000
VERSION     DAORHD010001449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131446_length_1391_cov_2.964072"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..>1391
                     /locus_tag="PKO06_22520"
     CDS             19..>1391
                     /locus_tag="PKO06_22520"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72501.1"
                     /translation="MSFFIMGLGQVYAGHIDRGLTLLIIQILSMSTGYSLYSKGLVYE
                     YLAGYLGPAGIVLIVYILLVFLIVLWVYNVKDAYYLALFADFRDWTEIEKVIGKFLGD
                     GQPRLLGHQGFSSNSPPAPQERRPVHSPEPVIVSAAPRPAMARVVDEEDVDLEPPVQP
                     ARPQTSDKKKKKTRSRHLTEEKLEELVETERLWRFPARATGMVVVLLILLGGGGYFWF
                     LSHQEIQNQRRPAVSFTADWDRMAKSGVATSPPGLLAADRAGSASTDLSAVAPVGSPV
                     WYESALTLMQSGERSAGLGQLEEGLSQYPAPVEVWKTYLSAKREQDVPEAYEEALGRF
                     LEKYPAEVDYWIILAKCQFDRREHVEASRSLLKALEVSPNHARANYWLGVIYRELNLP
                     DDALAPLLKAVKADPLNAEFNRELGMAYLDQFDRANARRYVMKALSVEPTNEDLKTLI
                     KQIDDAEL"
BASE COUNT          286 a          389 c          412 g          304 t
ORIGIN      
        1 ccgggatggg cagcgttgat gtctttcttt atcatgggtc ttggtcaggt ttacgccggt
       61 cacatcgatc gggggctgac cctcttgatc attcagattc tcagcatgtc gaccgggtac
      121 agtctgtaca gcaagggcct ggtgtatgaa tatcttgccg ggtatctcgg gccggcagga
      181 atcgttctga tcgtctatat cttgctcgtg ttcctgatcg tgctctgggt ctacaacgtg
      241 aaagatgcct attacctcgc tctcttcgcg gactttcgag actggaccga gatcgagaaa
      301 gtcattggca agtttcttgg cgatggtcag ccgcgcctgc tcggacatca ggggttcagt
      361 tcgaacagtc cacctgcgcc gcaggaaaga cgccccgtgc attcacctga accggtcatc
      421 gtctcagcgg caccgcgccc ggccatggcc cgagtcgttg acgaggaaga cgtggatctc
      481 gaaccgcctg tacaaccagc tcgtccgcag acatcggaca agaagaagaa aaagacccga
      541 tcacggcatc tcaccgaaga gaagctggaa gaactggttg agaccgagcg cctctggcgg
      601 tttcctgccc gggcaaccgg aatggtcgtc gtgctgctga ttcttctggg tggtggcggg
      661 tacttctggt tcctctcgca tcaggagatt cagaaccagc gccgaccggc tgtatcattc
      721 actgctgact gggacagaat ggcaaaatct ggagtggcga cctctccacc cgggcttctt
      781 gcggccgacc gtgcggggtc agcctccacg gatctctccg ccgtcgcccc cgttggatcg
      841 ccggtgtggt atgagagcgc tctgacgctg atgcagtccg gcgagcgatc ggcggggttg
      901 gggcaactcg aggagggttt gtcccagtat ccagcccccg tcgaggtctg gaagacctat
      961 ctctcggcca agcgcgaaca ggatgttccg gaggcatatg aagaagccct gggacggttt
     1021 ctggaaaagt atcccgctga ggtcgactac tggataattc tggccaaatg ccagttcgat
     1081 cgtcgtgagc atgttgaagc ttcgcggtct cttctcaaag cgctcgaagt ttcacccaat
     1141 catgccaggg ccaactactg gcttggggta atctatcgtg aactcaacct gccggacgat
     1201 gccctggcgc cgctgttgaa ggcggtgaaa gccgacccgc tcaatgcgga attcaaccgc
     1261 gagctcggaa tggcctatct cgatcagttc gatcgggcaa atgcgcgccg atatgtcatg
     1321 aaggcgttga gtgtcgaacc cacgaacgaa gacctgaaaa cgctgatcaa gcagatcgat
     1381 gacgccgaac t
//
LOCUS       DAORHD010001450         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131581_length_1390_cov_3.435206, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001450 DAORHD010000000
VERSION     DAORHD010001450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131581_length_1390_cov_3.435206"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PKO06_22525"
     CDS             complement(<1..946)
                     /locus_tag="PKO06_22525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNV72502.1"
                     /translation="MNGPLHGLVVVDLTRVLAGPFCTMLLADMGAEVLKIERPEGGDD
                     SRAFGPFVKGESAYFMSINRGKKSATLNMKHPEGKKMLKELIAGADVLVENFKPGVMS
                     RLGLGWEHLKELNPRLVYAASSGFGQTGPYSERPAYDLIIQGMGGLMSITGPDAQHPT
                     KVGSSVADIFAGIFSAVGILAALYSRERTGRGQQVDVGMLDCMVAILENAISRFVTSG
                     KNPEPIGNRHPSIAPFCTVTTADGAMNIACGNDELWRKFCLLTGLNELSADERFATNP
                     SRVEHFEALEPLINATTRSRPTAEWLSVLQAGGIPCGPI"
     gene            complement(949..>1390)
                     /locus_tag="PKO06_22530"
     CDS             complement(949..>1390)
                     /locus_tag="PKO06_22530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNV72503.1"
                     /translation="VICYDGDFPELARVTTLQGAEIICRPSAFMRTYDHWELTNRARA
                     YDNHVYWIATNAVGVDASGAYFFGSSMIVHPTGLKTAQARACDEFVWADLDPDPLRTV
                     LPNTSSPQIFDHLEDRNLSSYVGILTQGRSAFEPAKRIPYHRWR"
BASE COUNT          286 a          392 c          434 g          278 t
ORIGIN      
        1 tgatcggccc gcagggaatc ccgcccgcct gcagaaccga gagccactct gcggtggggc
       61 ggctgcgggt ggtcgcatta atcaggggct cgagtgcttc gaagtgctcg acccgtgagg
      121 gattggtggc gaaccgttca tcggctgaaa gctcgttgag ccccgtcagt aagcagaact
      181 tgcgccagag ctcatcgttg ccacaggcga tgttcatcgc accgtcggct gtcgtgacgg
      241 tgcagaaagg tgcaatcgac gggtgccggt tgccgatggg ctcggggttc ttgccactgg
      301 tgacgaagcg tgagatcgca ttctcgagaa tcgcgaccat gcagtcgagc atgccgacat
      361 cgacctgctg accacgaccg gtacgttcgc gtgaatacaa agcggcaaga atgccgaccg
      421 cggaaaagat gcctgcgaaa atgtcggcca ccgaactgcc gactttcgtg ggatgttgcg
      481 catcgggacc agtgatgctc atcaaaccac ccattccttg aatgatcaga tcatacgcgg
      541 ggcgttcact atacggtccg gtctggccga aacctgacga ggcggcatag acgagtcgcg
      601 ggttgagttc tttcagatgt tcccacccaa gtccgaggcg gctcatgacg ccaggtttga
      661 agttctcaac caggacgtct gctccggcaa tcagctcctt gagcatcttt ttcccttccg
      721 ggtgcttcat gttcagcgtc gcgctctttt tcccgcgatt gatgctcatg aaatacgcgc
      781 tttctccctt gacgaaaggc ccgaaggcgc gcgaatcgtc cccaccttcg gggcgctcga
      841 tcttgagcac ttcagcgccc atgtcggcga gtagcatagt acagaatggc ccggcgagaa
      901 cgcgggtcag atcgacgacg acaagtccgt gaaggggtcc gttcatgatt accgccagcg
      961 atgataggga atgcgcttgg ccggctcgaa ggccgagcgg ccctgcgtca gaatgccgac
     1021 atacgaagac aggttgcggt cttcgagatg atcgaaaatc tgaggtgaag aggtgttggg
     1081 aagcacggtg cgcagcggat cggggtcgag gtcggcccag acaaactcat cgcaggctcg
     1141 ggcctgcgcc gttttcagac cggttgggtg cacgatcatc gatgagccga agaaataggc
     1201 accgctcgcg tcgactccga ccgcattggt cgcgatccag tagacgtgat tgtcatacgc
     1261 gcgggcgcga ttggtaagtt cccaatggtc gtaggtgcgc atgaacgccg acggtcggca
     1321 gatgatctcg gccccctgca gcgtggttac gcgcgccagt tctggaaaat cgccgtcata
     1381 acagatcacg
//
LOCUS       DAORHD010001451         1390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131619_length_1390_cov_2.713858, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001451 DAORHD010000000
VERSION     DAORHD010001451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131619_length_1390_cov_2.713858"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..668)
                     /locus_tag="PKO06_22535"
     CDS             complement(297..668)
                     /locus_tag="PKO06_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNV72504.1"
                     /translation="MTRSDVYKCDVCGNVIAVMHVGADALVCCGKPMRQMVENTVDAA
                     KEKHVPVVEKAGQGDQVKVGSVTHPMEEKHYIEWIEVIDGDRHYIRYLKPGEKPEADF
                     PVSGNNLKVREYCNLHGLWKA"
     gene            complement(790..>1390)
                     /locus_tag="PKO06_22540"
     CDS             complement(790..>1390)
                     /locus_tag="PKO06_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV72505.1"
                     /translation="EDMNMLASLMEEVEYEPKELIVGEKQETTHLYFLLSGRAQIYKM
                     SSGQVNILTVLEKNDLFGEVSFVDRQVRSACVSALDKCSVAILKLEHFDVIKKQAPAF
                     GMDLLMALMKELTRRFRAVNEGLDIKSPEYVIQELILSGQQVMVNTKSGQEYICIVKA
                     SDRSQSLPMLKIGIQSGQTLMIPFHEIKAIVFPNKFGKY"
BASE COUNT          289 a          401 c          373 g          327 t
ORIGIN      
        1 gggcgtgcaa ctgcatgatg atgggttgcc atccggcgga acgatcaaga tctccggcgt
       61 tgatccgctg cagaaaagca tgtcgctgct gatgcgcttc ccgtgcgaag tggtgggtga
      121 gccgttgccg ccgatcgata aacgcaaggc ggaaaaccgc gtctcgcgct gatctgaaac
      181 gcgagcgtcc tggacgctta gatatcgcct aaaaccacgg gggcgggttc atgtgaaccc
      241 gcccccgttg ggctaaagaa catccctcat ggtcgaaccc tccgcggatg tttggactac
      301 gccttccaga gcccgtggag gttgcaatac tcgcggactt tcagattgtt cccgctgacc
      361 gggaagtcgg cttctggctt ctcgcccggt ttcagatacc ggatatagtg ccgatcaccg
      421 tcaatcactt cgatccactc gatataatgc ttctcttcca tcgggtgcgt cacgctaccg
      481 actttcacct gatcgccctg accggctttt tcaacgaccg gcacgtgctt ctctttcgcc
      541 gcatcgacgg tgttttcgac catctgccgc atgggcttgc cgcagcacac aagagcgtcg
      601 gcgccaacat gcatgacagc gattacattg ccgcagacat cacatttata cacgtcagaa
      661 cgggtcatgg gtttcctcca ggtgaatttc ggtcgagggg attatacgag tcgacgtacg
      721 cagggtcaat aggccgccaa actctgactc agtgccgatt gatggcgtgg tacaccggct
      781 cttccgctct cagtactttc caaatttatt cggaaaaaca atcgccttga tttcgtgaaa
      841 cggaatcatc agcgtctgcc ccgattggat cccgattttc agcatgggaa gagactgtga
      901 acgatcagag gccttgacga tgcagatgta ttcctgcccg ctcttcgtgt tgaccatgac
      961 ctgctggcca ctcagaatca gctcctgaat gacgtattcc ggcgacttga tgtcgagccc
     1021 ttcgttgaca gcgcggaagc gacgggtgag ttccttcatc agggccatca gcagatccat
     1081 gccgaaggca ggagcctgct tcttgatcac gtcgaaatgc tcgagcttga gaatggcgac
     1141 gctgcacttg tccagcgcgc tgacgcaggc ggagcgcacc tgccggtcga cgaacgagac
     1201 ttccccgaac aggtcgttct tttcgagcac cgtcagaatg ttcacttgtc ccgaggacat
     1261 tttgtagatc tgtgcgcgcc ccgacaaaag gaaatagagg tgcgtggttt cctgcttctc
     1321 gccgacgatc agctctttgg gctcatattc gacttcctcc atcagggaag ccagcatgtt
     1381 catatcttcc
//
LOCUS       DAORHD010001452         1389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131786_length_1389_cov_2.611694, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001452 DAORHD010000000
VERSION     DAORHD010001452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131786_length_1389_cov_2.611694"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKO06_22545"
     CDS             complement(<1..345)
                     /locus_tag="PKO06_22545"
                     /inference="COORDINATES: protein motif:HMM:NF013898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+ dependent nucleoside transporter N-terminal
                     domain-containing protein"
                     /protein_id="HNV72506.1"
                     /translation="MEKAISLLGIAVQIAILYALSTNRKKINWTLVAMGIGLQFGFAL
                     IILKTTPGEQFFKYMNDIIVGLLGFTQRGAAFVFGSMTEPNIPVGIPKAGPPAGFADL
                     LPIPGQTLMARPG"
     gene            complement(537..>1389)
                     /locus_tag="PKO06_22550"
     CDS             complement(537..>1389)
                     /locus_tag="PKO06_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72507.1"
                     /translation="TPVEMLVIRSAASLSETLRQTFPPFLQEGTNAASTPATRVFFPR
                     GDQPVQPAIDAFVGALQAFQADAKNLNETFRARMRAMRLDTQARIGLFTLITVFCALV
                     LAFFFSRQIALPTQRVIDLINRIRDGQYVNLNIRDQTEDEIGRIFGALLHMVSHIATR
                     DQLKLEKIDLEKRRFEVFGNQIKVPFLLLNRDRQVAFANHACLELFRLAWDDVYEIEL
                     AKIAIPPQLKEEISSAITAQKWNEERPLELIGENYAFDLNLRLIPVKGHNSDTQSVIC
                     FLQKMRD"
BASE COUNT          313 a          363 c          419 g          294 t
ORIGIN      
        1 acccggacgc gccatcaggg tctggccggg aatcggcagc agatctgcaa aacctgcagg
       61 tggaccggct tttggaatac cgaccgggat gttgggttcg gtcatactgc cgaacacgaa
      121 ggccgcaccc cgttgtgtaa aaccgagcaa cccgacgatg atgtcgttca tgtacttgaa
      181 aaactgttcg cccggggtgg ttttcagaat gatgagcgca aacccgaact gcagaccaat
      241 gcccatggca accaaggtcc agttgatctt cttgcggttt gtcgacagag catagaggat
      301 ggcgatctga acggcgatgc cgagcaaact gatggctttt tccacgggta cggacctcct
      361 ggcggtgttg cccgtcggct cagaagcgag aaaaccaacg agaccgcagc atcatatcag
      421 aaagcggcgc acagttcacc agaaatgcgg gtagctggac acctcgccac gcaaaactga
      481 tgtgatccga gtttccgtga atggtgtctc acgggatgcg ccgttgtgcg tggtcgtcag
      541 tcgcgcattt tttgcagaaa acagatcacg gactgggtgt cgctattgtg ccctttcacc
      601 gggatcaggc ggagattcag atcaaaggca tagttttcac cgatcagttc aagaggtctt
      661 tcttcgttcc acttctgtgc cgtgatcgca ctcgaaatct cttctttcag ttgcggggga
      721 atggcgatct tcgcgagttc gatctcatag acgtcatccc acgccagacg aaacagctcg
      781 aggcatgcgt ggttcgcaaa agccacctgg cgatcgcgat tgagcaggag gaaagggact
      841 ttgatctggt tgccgaacac ttcgaaacgc cgcttctcga ggtcgatttt ctcgagcttg
      901 agttgatctc gagtggcgat atggctgacc atgtgcagca gggctccgaa gatgcgcccg
      961 atctcgtctt cggtctgatc gcgaatgttc agattgacat actgaccgtc tcgaattcga
     1021 ttgatcagat cgatgacccg ctgtgtcggc aaggcgatct gtcgcgaaaa gaagaacgcc
     1081 agaaccagcg cgcagaagac ggtgatgagc gtaaacagcc cgatgcgagc ctgggtgtcg
     1141 agacgcatcg cccgcatgcg ggcgcgaaac gtttcattga ggttcttcgc atcagcctga
     1201 aaggcctgca gtgcgccgac aaaagcatcg atggccggct gtaccggctg gtcaccgcgg
     1261 gggaaaaaca cgcgggtggc gggtgtcgat gcggcgttgg taccttcttg cagaaacggg
     1321 gggaacgtct gtcgcagggt ctccgagagt gaggcggcag agcgaatcac cagcatttcg
     1381 accggcgtt
//
LOCUS       DAORHD010001453         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_131915_length_1388_cov_2.809452, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001453 DAORHD010000000
VERSION     DAORHD010001453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131915_length_1388_cov_2.809452"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..1007)
                     /locus_tag="PKO06_22555"
     CDS             complement(330..1007)
                     /locus_tag="PKO06_22555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV72508.1"
                     /translation="MKTVPTREGYDLWATCYDTEDNCLIVLEEQHIAPFFADARGKRI
                     LDLGCGTGRHSFALARQGAHVIGLDFSDGMLRLAREKSAPGALPPQFVSGDLHEPLPF
                     HHRQFDAIGCFLVIEHIRDRVGLFREMRRVCRPGGTIILSAMHPAMFLRQTQAGFPDP
                     HTGCKIQPESHPHSISDLFEAGRHAGLTLLDLREAAVTPELVAQSPKAAKYLGWPLLL
                     LFRWQAP"
     gene            complement(1060..>1388)
                     /locus_tag="PKO06_22560"
     CDS             complement(1060..>1388)
                     /locus_tag="PKO06_22560"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNV72509.1"
                     /translation="FEERSARITDNEVQAVIDQEELIWRKFKQSKDLSVHLDVMKKAM
                     NLLRRHAEGKGGLSAQTVSAITAAFQYVLDPMDVIPDAIPEVGYLDDVVVIEKCLEII
                     APVLKS"
BASE COUNT          295 a          366 c          451 g          276 t
ORIGIN      
        1 tcttttttct cggtctggcg cgcggcctca tcggggttct ctgctccggc tcggcaggcc
       61 agactcaaga cgaatgccga aagaaacaac acgcacagga cgatcagggg aaaacgcctc
      121 atcatttcac ctccaggcag attttttcac aggatatcac acggatgcgt gattcgctga
      181 aatgagacgt cgggtcgcga ggtgaagggc tcctgaatgc gtggagcggc gagccatcgg
      241 aaacattcag gttgaatggc ggaaaaggag cggcgcgacg aggaaaagcc cggcaatgat
      301 tgccgtcgct ttcgtgggtt gaggtgccct cagggggctt gccagcgaaa caagagcagc
      361 aggggccatc cgagatactt ggcggccttg ggtgactggg cgacgagttc gggggtgaca
      421 gcggcctcgc gcaggtcgag cagggtgaga ccggcatgac gaccggcttc aaagaggtcg
      481 ctgatcgagt gcggatgact ctcgggctgg atcttgcacc cggtgtgcgg gtcgggaaag
      541 cccgcctggg tctggcgcag aaacatggcc ggatgcatgg cggacagaat gatggttccc
      601 ccggggcggc agacgcgtct catctcacgg aacaacccga ctcgatctcg aatgtgctcg
      661 atgaccagaa aacacccgat ggcatcgaac tgtcggtggt gaaagggcag tggctcgtgc
      721 aggtcacccg agacaaactg aggcggcagc gcacctggag cggacttttc ccgggccagt
      781 cgcagcatgc cgtccgaaaa gtccaggccg ataacgtgcg ctccttgcct ggcaagagcg
      841 aaagagtgcc tgccggttcc gcagccgaga tcgagaatgc gcttgccgcg agcgtcagca
      901 aagaagggcg cgatgtgctg ttcctcgagc acgatcaggc agttgtcttc ggtgtcgtag
      961 caggtggccc acaggtcata tccttcgcgg gtcggcaccg tcttcacggg gcggggatca
     1021 agaggcggct ggttcatgag tgagaagagg ggcgaatagt cagctcttca ggaccggagc
     1081 tatgatttcg aggcactttt cgatcaccac gacatcgtcg aggtacccga cctcgggaat
     1141 ggcatcggga atgacatcca tcggatcgag cacatattga aaggcggcgg tgatagccga
     1201 caccgtttgt gcgctcaacc cgccttttcc ttccgcgtgt cggcgaagca ggttcatggc
     1261 ctttttcatg acatcgaggt gaacggatag atcttttgac tgtttgaact ttcgccagat
     1321 aagctcttct tgatcaatga cagcttgtac ttcgttgtcg gtgatccggg cggatcgttc
     1381 ctcgaaat
//
LOCUS       DAORHD010001454         1387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_132044_length_1387_cov_3.450450, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001454 DAORHD010000000
VERSION     DAORHD010001454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132044_length_1387_cov_3.450450"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="PKO06_22565"
     CDS             <1..1327
                     /locus_tag="PKO06_22565"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV72510.1"
                     /translation="AAIALLGASPLAVLVFEVVLLGTFRDVPQTILSEFFLVMIWGSL
                     IGICGALTLYLLFRHHLAPEYLHNPLTLALVLGTFALSNQIQAESGLLAVTVTGILLG
                     YQRSVPVRHILQFKENLQVLLISVLFILLASRLRLSDLSVIDPSMLLFIALLIFVVRP
                     VAVWLSTIGSDLSHHEIWYLSLFAPRGIVAASVASIFSLKLTEAGNHNAYLLVPYCFA
                     VIIVTVCFYGLASGPLARRLGVSQRNPQGTLLIGAHDWARDMARRLQELGIPILMLDR
                     NTFNILEARRAGLPVLEKSCLAEDISEEIELSSYRRLLCLTSNDNVNALAQMRFQEFF
                     DRLDLYQLPLELTTEMPVDFCGRFLFSPQATFLEISQRYARGHRFRIFLANEWQAFIS
                     RPPVPNEVATQPIVFFRLDARNRLEVLLDPGSLRVQAEDRLVVLCAPEG"
BASE COUNT          222 a          447 c          362 g          356 t
ORIGIN      
        1 cgcggcgatt gcgctgctcg gcgcgtctcc actcgccgtg ctggtgttcg aagtcgttct
       61 gctggggacc tttcgcgacg ttccgcagac cattctgtcc gaatttttcc tggtcatgat
      121 ctggggcagc ctgattggca tctgcggagc gctcactctg tatctcctgt tccgccatca
      181 cctcgccccc gagtatctgc ataatccgct gactctggcc ctggtactcg ggacctttgc
      241 gctgtcgaat cagattcagg ctgagtcagg acttctggcg gtcaccgtca ccgggattct
      301 gctcgggtat cagcgctctg tgcccgtgcg gcatattctg cagttcaagg agaatctgca
      361 ggtcctgctc atctccgtgc ttttcatcct gctggcttca cgcctgcggc tttctgatct
      421 ctcggtgatc gacccgagca tgctgctctt catcgccctg ttgatcttcg tggtgcgtcc
      481 tgttgccgtc tggctttcca ccattggcag cgatctgagc caccacgaaa tctggtatct
      541 ctctctcttt gcgccacgcg gtatcgtggc tgcttccgtc gcttccatct tctcgctcaa
      601 gttgaccgaa gccggcaacc acaatgccta tctgctggtg ccttactgct tcgcggtcat
      661 catcgtcacc gtctgtttct atggtctcgc ttcaggtcca ctcgcccgac gtctgggcgt
      721 ttcgcagcgg aaccctcagg gcactctgct catcggcgct catgactggg ctcgcgacat
      781 ggcccggcga ctgcaggaac tcggaattcc cattctgatg ctcgatcgca acaccttcaa
      841 cattctcgaa gcacgtcgcg ccggcttgcc ggtgcttgaa aagagttgcc tggcggaaga
      901 tatctcggag gagattgaac tcagctcgta ccgtcgtctg ctctgtctga cctcgaacga
      961 caacgtcaat gccctggcgc agatgcgatt tcaggagttt ttcgaccgcc tcgatttgta
     1021 ccagcttcca ctcgagctca cgaccgaaat gcccgttgac ttctgtggtc gttttctctt
     1081 ttcgcctcaa gccacgtttc tcgagatcag tcagcgatat gcgcggggcc accgtttccg
     1141 catctttctc gcgaatgagt ggcaggcctt catcagtaga ccaccggtgc ccaacgaagt
     1201 cgcgacccaa cccatcgtgt tcttccgact cgatgccaga aaccgacttg aggtgcttct
     1261 cgaccccggc agcctgcggg ttcaggcgga agaccgcctc gtcgtgttgt gtgcgcccga
     1321 aggctgaaca accacccccc ttgttgagga gaggatcatg aatactcccg gagccatgcc
     1381 cgctgcc
//
LOCUS       DAORHD010001455         1386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_132258_length_1386_cov_2.728024, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001455 DAORHD010000000
VERSION     DAORHD010001455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132258_length_1386_cov_2.728024"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="PKO06_22570"
     CDS             complement(<1..1022)
                     /locus_tag="PKO06_22570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNV72511.1"
                     /translation="MSDRWAFSPQNPELRVVTTQGIRRRVDGESEWLEVETGVLRQLA
                     AEAFRDLAFYLRPSQLEAWAVICRDPLASDNDRFVAAQLLRNAVISAEGVLPLCQDTG
                     TATIFARRGYRVITAGTDESELLAGVGDAWSEHCLRASQMVPTTLFVESNSGNNLPAM
                     VEIRAVAGTEYELLFMAKGGGSANRTMLFQKTPAILNESAFETFLREQLRELGVAACP
                     PYRLAVVVGGLSPEHNLETLKLATAEALDHLPGAASGATAFRDIAWEERVCRLAAEQP
                     AGAKFGGRWFALSARVIRLPRHAASCFISIGVSCSAHRQMIGRVTAEGAFLERLESAP
                     SRFMPDA"
     gene            1120..>1386
                     /locus_tag="PKO06_22575"
     CDS             1120..>1386
                     /locus_tag="PKO06_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV72512.1"
                     /translation="MTPAPSAASDDPVEVPINGELDLHTFRPEEVGELVPDYIEACLE
                     RGIYSLRIIHGKGKGHLRRSVQAILSRSPHVESFSLADESAGSWG"
BASE COUNT          294 a          438 c          393 g          261 t
ORIGIN      
        1 gcgtcaggca tgaaccgcga tggcgctgac tcgagacgtt caagaaatgc gccctcggca
       61 gtcacccggc cgatcatctg acgatgagcg ctacacgaga ccccgatcga gatgaagcac
      121 gaagcggcat gacgcggcaa acggatcacc cgcgcgctga gcgcgaacca gcgaccgccg
      181 aacttcgccc cagcaggctg ttccgcggcc aatcggcaca cccgttcttc ccaggcgatg
      241 tcacgaaacg ctgtggcacc gctggccgca cccggcagat gatcaagcgc ttcggccgta
      301 gccagcttga gggtttcgag attgtgttcg ggtgagagtc cgccgacaac gaccgccagg
      361 cgatacggcg ggcaggccgc gactccgagt tcacgcaact gctcgcgcag aaacgtctcg
      421 aatgccgact cgttgagaat agccggtgtc ttctgaaaca gcatcgttcg attcgctgat
      481 ccgccgcctt tggccatgaa tagcaactcg tactctgttc cggcgacggc tcgaatctcg
      541 accatggcgg gaagattgtt gccagagttg ctttcgacga acagggttgt cggaaccatc
      601 tgcgaagccc gcagacaatg ctccgaccag gcatccccta ccccggccag aagttcactc
      661 tcgtcagtac ccgccgtgat cacccgataa ccgcgtcggg cgaatatcgt cgctgtgccg
      721 gtatcctggc acagaggcag cactccttcg gccgaaatca cggcatttcg caacagctgc
      781 gcagccacga accggtcatt gtcacttgcc agcggatcgc gacagataac cgcccaggct
      841 tcgagttgag acgggcgcag gtagaatgcc agatctcgaa aggcttctgc cgccaattgc
      901 ctcagaacgc cggtttcgac ttcaagccac tcggactcgc catcgacgcg acgacgaatg
      961 ccctgggtcg tcaccacgcg cagttcgggg ttctgcggcg aaaaggccca gcggtcactc
     1021 acgatgtgta gcctccgtca gaaagtcatg atggtgtatg atacaggtat caggtttcat
     1081 catgcacgca caacgtcacc caacctctgc gaggacttta tgactcctgc cccatcggcc
     1141 gcttcagatg atcccgtcga ggttccgatc aacggggagc ttgacctgca cacgtttcgt
     1201 cccgaggagg tcggcgagct ggttcccgac tatatcgagg cctgtctcga acgcggtatc
     1261 tactcactgc gcatcatcca tggcaaaggg aaaggacatc tgcgacgaag cgtacaagct
     1321 atcctcagtc gttcgcctca tgttgagtcg ttttcactgg ccgacgagtc agccggcagt
     1381 tggggc
//
LOCUS       DAORHD010001456         1385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_132367_length_1385_cov_3.362406, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001456 DAORHD010000000
VERSION     DAORHD010001456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132367_length_1385_cov_3.362406"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PKO06_22580"
     CDS             complement(<1..509)
                     /locus_tag="PKO06_22580"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV72513.1"
                     /translation="MPHNDNQELVTRVTSAVRSALSDAEGGHDWWHTHRVWQMACRLA
                     QAERAELLIVELGALLHDVADAKFHDGDETAGPRKTEAILTNCGATKPVIDTVRNLVA
                     HVSFKGGNHVQEFRSLELDVVQDADRLDALGAIGIARAFSYGGYRGTPLHDPEIKPRL
                     RMTREEYRS"
     gene            complement(526..1179)
                     /locus_tag="PKO06_22585"
     CDS             complement(526..1179)
                     /locus_tag="PKO06_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72514.1"
                     /translation="MNKVMHQFESRLSKQELKSLSGLTTPFRIQEYLDQVGYSDDEFY
                     RCPLRVMRDRKAHCFDGAVFAAMALRRLGLPPLLIELLPNDRDDDHLLALFKIDGCWG
                     AIGKSNFSGLRYREPIHRTLRELVLSYFEPYYNVAYEKTLRGYTVPMNLSRFDRSDWM
                     ANDEPLYQIADALDTMRRYSLLTKAMEKRLNPVDERSYQAGLMGSLASGLYNPLKQS"
BASE COUNT          290 a          413 c          371 g          311 t
ORIGIN      
        1 tgcgaccggt actcttcccg ggtcatacgc aggcgtggct tgatctccgg gtcatggagc
       61 ggcgtcccgc ggtatccccc ataactgaac gctcgagcga ttccgatagc tcccagtgcg
      121 tccagtctat cggcgtcctg aaccacgtcc aattcaagtg atcgaaactc ctgaacgtga
      181 tttccacctt tgaaagaaac atgggcaacg aggtttctga ccgtgtcaat gaccggcttt
      241 gtcgccccac agttcgttaa aatcgcttcg gtttttcggg gtccggcggt ttcatccccg
      301 tcgtgaaact tggcatcagc gacatcatgc agcaaggctc caagttcgac gatgagcagt
      361 tcggcccgtt cggcctgcgc caggcgacaa gccatctgcc agactcggtg cgtgtgccac
      421 cagtcgtggc caccttccgc gtcactcaag gcagagcgaa cggcagacgt cactcgcgtg
      481 accaactcct gattgtcgtt atgcggcatg cgcaggcgac agctctcagg actgtttcaa
      541 cggattgtac aaaccgctgg caagtgaacc catcagcccg gcctggtatg agcgttcgtc
      601 aaccggattc aagcgttttt ccatcgcctt ggtcaacagg gaatagcgac gcatggtatc
      661 gagcgcatct gcgatctgat acagcggttc gtcattggcc atccagtccg agcgatcgaa
      721 acgggaaaga ttcatgggaa cggtgtaccc gcgcagcgtt ttctcatacg cgacattgta
      781 gtacggttca aagtagctga gcaccagctc gcgcagcgtt ctatggatcg gttcgcgata
      841 gcgcagcccg ctgaagttcg atttgccgat cgccccccag cagccgtcga ttttgaacag
      901 ggccagcaga tggtcatcat cacgatcgtt cggcagcaac tcgatcagca acggtggcag
      961 gccgagacgg cgcagagcca tagccgcaaa caccgctcca tcgaaacagt gggccttgcg
     1021 atcgcgcatg acccgcaacg gacagcgata gaattcatcg tccgaatacc caacctggtc
     1081 caggtattcc tgaatccgga acggggtcgt cagcccgctc aggcttttga gctcctgctt
     1141 cgacaaacgt gattcgaact ggtgcatgac tttgttcatc gtgttgctcc cgaaagaagg
     1201 tgttcgtcag tttacttgct ctctgtcgcg atgtcacgca ccctgctcgt ctttcccgtt
     1261 caagccgaca cgcaggctca ccaaccacag gagtttgttc gcccggggtg ctcgattttc
     1321 gcagcagacc cagggtcatt taattctcgc ttattttccg ttcgcaagag cgaaacttgt
     1381 gagcc
//
LOCUS       DAORHD010001457         1382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_132848_length_1382_cov_3.298417, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001457 DAORHD010000000
VERSION     DAORHD010001457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132848_length_1382_cov_3.298417"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PKO06_22590"
     CDS             complement(<1..659)
                     /locus_tag="PKO06_22590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72515.1"
                     /translation="MLRKSWLLCFALVLWAGSSALPVFAQASLADGDTLDLAISIDGV
                     KEGVTCIRDAVKPNQWYYVPKMIRLDTVMDPNLKKERPQFALVKYQGKDPDDPQKLLE
                     GGVLQCSFNLSLPGKSVTELRKKLAEHANVAEKDLLLTPLSMSDGSLSIYTPGGDKMG
                     EAPQAPDIAPTFANQSIPVQINLTRLGADFVDALTKSGGIQVYVVFNYNGLTPKAGFR
                     I"
     gene            complement(731..>1382)
                     /locus_tag="PKO06_22595"
     CDS             complement(731..>1382)
                     /locus_tag="PKO06_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72516.1"
                     /translation="TPETELQRVDLQVRLLYRGQEYERFLLQWMPNSGWQDQDGIPRQ
                     TLKIPLKNLVAADAKALDNAQLELTQTLQLATNDRLTTVQQVPVCTGDLPIATPLETV
                     QALALDFSLLDWTALETDRSRLIRTEVRIRQGTRQLLRSLKPERKAQGKLVIPGIVQW
                     LVPATSLEKPGQIEVNVFFYTADGRRIPWALNGRPLEQDFTTGVWYFTDEDWKGTT"
BASE COUNT          288 a          382 c          412 g          300 t
ORIGIN      
        1 ttgatgcgga acccggcttt cggggtaaga ccgttgtagt tgaataccac gtagacctgg
       61 atgccgccgg acttggtgag cgcatcgacg aaatccgcgc cgagcctggt caggttgatc
      121 tgcacaggaa tcgactgatt cgcgaacgtc ggggcaatgt cgggagcctg gggtgcttcg
      181 cccatcttgt caccgcccgg ggtgtagatg gaaagactgc cgtcagacat ggaaagcgga
      241 gtgagcagca ggtctttctc ggcaacgttg gcgtgttcgg cgagcttctt gcgcagctcg
      301 gtcactgatt tacccggcag cgacaggttg aaggaacact gcagtacgcc gccttcaagc
      361 agcttctgcg gatcgtcggg atccttgccc tgatatttga ccagggcgaa ctgcgggcgt
      421 tccttcttca ggttggggtc catgaccgtg tcgagccgaa tcatcttggg cacgtagtac
      481 cattggttcg gtttgaccgc gtcacggatg caggtgacgc cttctttcac gccatcgatg
      541 ctgatggcga gatcgagcgt gtcgccatcg gccagactgg cctgcgcgaa caccggaagg
      601 gcactactgc ctgcccacag gacaagagca aaacacagca accaactctt acgtaacatt
      661 ccgtccactc ccttatgatt atttgatgag cccctagagg ggcagtaacc cgtaccaaaa
      721 aagaagatct tcaggttgtg cctttccaat cttcatccgt gaaataccac acgccggtgg
      781 tgaaatcctg ttcgagcggg cgcccattca acgcccaggg aatacgccgc ccgtccgcgg
      841 tatagaaaaa gacgttcacc tcgatctgtc ccggcttctc cagagacgtc gctgggacaa
      901 gccattgcac gattcccgga atgaccagtt tcccctgagc cttgcgttca ggtttgagag
      961 agcgcagcag ttgacgggtg ccttgccgaa tgcgcacttc ggtgcgaatc aaacgtgaac
     1021 gatccgtttc cagggcggtc caatccagca ggctgaagtc aagagcgaga gcctgcacgg
     1081 tttcgagtgg agtggctatc ggcagatcgc ccgtgcagac cggcacctgt tgcacggtgg
     1141 tcaggcgatc gttcgtcgcg agttgcaacg tttgagtcag ttcgagttgt gcgttgtcga
     1201 gagccttggc atcagcggcg acgagattct tcaaggggat tttcagggtc tggcggggaa
     1261 taccgtcctg gtcctgccag ccggaattcg gcatccattg caggagaaaa cgctcgtatt
     1321 cctgaccacg atagagaagc cgtacctgca gatcaactcg ctgaagttcc gtctccgggg
     1381 tg
//
LOCUS       DAORHD010001458         1381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_132995_length_1381_cov_3.629713, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001458 DAORHD010000000
VERSION     DAORHD010001458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132995_length_1381_cov_3.629713"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..942)
                     /locus_tag="PKO06_22600"
     CDS             complement(52..942)
                     /locus_tag="PKO06_22600"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72517.1"
                     /translation="MKLSKHPNPEFVDRLLDLRALLARKSCFLFGPRQTGKSLLIKYS
                     LPECRAYNLLDSATFLKLSQNPGRIAQEIVPGDEIIVIDEVQRLPELLNEVHLLIEER
                     GLRFLLIGSSSRKLRRGGTNLLGGRARTQAFHPLTWKELGIGFDLERALERGLIPSHP
                     FSDSPREDLQAYAGSYLQQEIMAEGATRNIPAFSRFLKVAAHCNGTVVNFTNIANDAQ
                     VSRTTVYEYFEILKETLLIHELPAWRKTTRRKPLATSKFYFFDIGVRGRSHYRRSHSG
                     RVQSESHRFRQRSQITSSTG"
     gene            complement(1073..>1381)
                     /locus_tag="PKO06_22605"
     CDS             complement(1073..>1381)
                     /locus_tag="PKO06_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019893070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HNV72518.1"
                     /translation="AALKAQVEHHYPEAKVTTATADTLFDCRIMVKQILAKSLTANLV
                     EGMACPGGCVGGPGTLAPIRKSTAEVGKFVKTTPIQGAWEAPDHPVHPPEPVPPKSDH
                     "
BASE COUNT          284 a          384 c          419 g          294 t
ORIGIN      
        1 cgtggcaccc cttcgcggca gacgcacagg taccgcttaa gctttccctc ttcagccagt
       61 gctcgaagtg atttgagatc gttgtcggaa acggtggctt tcgctttgaa ctcgaccgct
      121 gtgtgatcgc cgataatgag atcgacctcg aaccccgatg tcgaagaagt agaacttcga
      181 ggtcgccaga ggcttgcggc gagtggtttt gcgccaggcg ggcaactcat gaatcagcaa
      241 agtctctttg aggatctcaa aatactcata caccgtggtt cgggaaacct gggcatcgtt
      301 ggcgatgttg gtaaaattga ccaccgtacc attgcaatga gccgccacct tgagaaatcg
      361 gctgaacgcc gggatgttcc tcgtcgcgcc ctccgccatg atctcttgct gaagatagga
      421 tcctgcgtat gcctgaaggt cctcccgcgg agagtcggaa aaagggtgtg aagggatcag
      481 cccgcgttcc aacgcccttt cgagatcgaa acctattccg agttccttcc aggtcagcgg
      541 gtggaatgcc tgggtgcggg ccctgccccc gagaagattg gtaccgcctc gccgaagctt
      601 ccgagaactg gacccgatca ggagaaacct gaggccccgt tcttcgatga gcagatgaac
      661 ttcgttcaac agttcaggaa gacgctgaac ctcatcgatc acaatgattt cgtcaccggg
      721 tacaatctcc tgggcaatgc gaccgggatt ctggctcaac ttcaggaagg ttgcactgtc
      781 cagcaggttg taggcgcgac attccggcag agagtacttg atgagcaagc tctttcccgt
      841 ctgacgcggc ccgaagagga aacaggactt tcgcgcgaga agtgcgcgca ggtcaaggag
      901 acgatcgaca aattccggat tcggatgctt tgacaacttc atcgtcattt gtcagcaaac
      961 aagccattcc ctgttccgtg agcggttgcg acgagagttc ccgctgagtc atgcctatgg
     1021 gcccttggat aggagagcgc ttcagcggac gttgcaggca gtacccgctg ggtcagtggt
     1081 cagacttcgg tggcacgggc tcgggaggat ggacggggtg gtcgggggct tcccaggcgc
     1141 cctggatcgg cgtggtcttc acgaacttgc cgacctcagc ggttgatttg cgaatcggag
     1201 cgagcgtgcc ggggccgccg acgcagccac cgggacaagc catgccttcg accaagttgg
     1261 ccgtgagcga cttcgccaga atctgcttca ccatgatgcg acagtcgaac agcgtgtcgg
     1321 ccgtcgcggt ggtgaccttc gcttccgggt agtggtgttc gacctgcgcc ttcagcgcgg
     1381 c
//
LOCUS       DAORHD010001459         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_133203_length_1380_cov_2.956226, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001459 DAORHD010000000
VERSION     DAORHD010001459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133203_length_1380_cov_2.956226"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PKO06_22610"
     CDS             complement(<1..850)
                     /locus_tag="PKO06_22610"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV72519.1"
                     /translation="MSVQTSLIVKLWGVRGSVPTPLSSDDIRIKQVELLKRLYADGGS
                     DKLFGNPPDQARIDQWLQSLSPAITGTCGGNTTCFEVRGLNSPLIMIDAGTGARAMGQ
                     YLLKRLFSKQPLNPLNTVKASERDIHLFFSHYHWDHLQGFPFFGPAFTYGASKINFIF
                     YGRRDAKQRLSEVLGGQQECPNFPVAWEDMPCNKTFVEMGRLDPHPMQIGDVTVNYQE
                     LNHPDSVFGYAFTCNGKRFVYATDTEHRDMPDPRLMKLAQNADCLYYDSQYTPSEYRG
                     DQGSLTG"
     gene            964..>1380
                     /locus_tag="PKO06_22615"
     CDS             964..>1380
                     /locus_tag="PKO06_22615"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HNV72520.1"
                     /translation="MKKTRKHPIPARTAFIFDMDGTLVDNMAWHTRAWVELLADHGRS
                     LDVAEFSARSGGRKSAEVVRELFGEQLSASEVTDLVNQKELLYRSLYRRHLKLLPGLR
                     RFLTRAVKLGIPMAVATAAPPRNISFLLDGLNLRPFF"
BASE COUNT          281 a          382 c          416 g          301 t
ORIGIN      
        1 tgccggtcag tgagccctga tcgccccgat actcagatgg tgtgtactga ctgtcgtaat
       61 acagacagtc tgcgttctgg gccagcttca tcaggcgcgg gtcgggcatg tcgcgatgtt
      121 cggtgtcggt ggcatacacg aaacgcttgc cattgcaggt gaacgcgtag ccgaagaccg
      181 agtcggggtg gttcagttcc tggtagttga cggtcacatc gccgatctgc atggggtgag
      241 ggtcaagacg acccatctcg acgaaggtct tgttgcaggg catgtcttcc caggcgacgg
      301 gaaagttcgg gcattcctgc tgaccaccga gaacttcgga aagacgttgt ttcgcgtcgc
      361 gtcgcccgta gaaaatgaag ttgatcttcg aagccccata cgtaaatgcc ggcccgaaga
      421 aggggaagcc ctgcaggtga tcccagtggt agtgcgagaa gaacaggtga atgtctcgtt
      481 ccgacgcctt cacggtgttc aacgggttga gcggttgttt ggaaaacagc cgttttaaaa
      541 gatattgccc catcgcacgg gctccggtgc ccgcgtcgat catgatcaaa ggcgaattca
      601 gaccgcgcac ttcgaagcag gtcgtattgc cgccgcaggt accggtgatc gcgggcgaca
      661 gagactgcag ccactggtcg attctggctt gatcgggtgg gttgccgaag agtttgtccg
      721 agccaccatc agcgtaaaga cgcttcaaaa gctcgacttg tttgattcga atgtcgtcac
      781 tggagagagg cgtcggcacg ctgccgcgaa caccccacaa tttgacgatc agactggttt
      841 gaactgacat ggcatcccct ccggcgattg tacgtttcac gcatggtagc agaaaacttc
      901 attggcgcaa acaacttcgt gatccgcttg tctcgcgcag gagaaacgga taaaatcgcc
      961 gccatgaaga agacacggaa acatccgatt cccgcccgca ccgccttcat ctttgacatg
     1021 gatggaacgc tggttgacaa catggcctgg cacacgcgcg cctgggtcga gctgctggcg
     1081 gatcatgggc gctcccttga tgttgccgag ttctcggctc gcagcggcgg ccggaaatcg
     1141 gcggaggtgg tgcgcgagtt gtttggtgaa cagctttcgg cctcagaagt cacagacctg
     1201 gttaatcaga aagagctgct gtaccggtct ctctaccgtc gtcacctgaa gctgctgccc
     1261 gggttgcgcc gcttcctgac tcgcgctgta aagctcggga tcccgatggc cgtggcgacc
     1321 gcggcgccgc cgcgcaacat ttctttcctg ctcgatggcc tgaacctgcg cccctttttc
//
LOCUS       DAORHD010001460         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_133412_length_1379_cov_2.703172, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001460 DAORHD010000000
VERSION     DAORHD010001460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133412_length_1379_cov_2.703172"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKO06_22620"
     CDS             complement(<1..184)
                     /locus_tag="PKO06_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV72521.1"
                     /translation="MPQLRKDPVLKQWVIISPERGKRPSDFKKAEQPPDDPKTCPFCE
                     GNEAMTPPETLSFRTAG"
     gene            complement(200..1195)
                     /gene="galT"
                     /locus_tag="PKO06_22625"
     CDS             complement(200..1195)
                     /gene="galT"
                     /locus_tag="PKO06_22625"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNV72522.1"
                     /translation="MPQLRKDPVTKRWVIILHEQAKTPADFQIPQPPRQQNKCPFCPG
                     HEDMAPPEVMSYRTPETLPNQPGWAVRVIPNKFPALQIEGDLDRTGIGVYDMMNGVGA
                     HEVIIESPNHDDGFHNYTVEQISRILRTYTDRYRDLRRDRRFRYILIFKNQGSAAGAS
                     LDHPHSQLIATPIIPKRAMEEVEGAKRYYYYKERCVFCDIIRQELSSGDRIIHENEQF
                     VAFAPFASRFPFETWIAPKDHKDSFGDIDSNEIEGLAGIMHATLRKLIKTLNNPPFNF
                     IIHTAPCDEHCTDYFHWHLEIMPRLTKVAGFEWGSGFYINPVAPERAARFLINQE"
BASE COUNT          297 a          293 c          397 g          392 t
ORIGIN      
        1 ttccggcggt gcgaaacgac agggtttcgg gcggtgtcat cgcttcattg ccttcgcaaa
       61 aaggacaggt tttagggtcg tcaggaggtt gttctgcctt tttaaagtca gaggggcgtt
      121 tgccgcgttc gggcgaaatg attacccact gtttaagaac gggatctttg cgaagttgtg
      181 gcacgaattg gtctcctgac tattcctggt tgatcagaaa tctggcggcg cgttcggggg
      241 ctacgggatt tatgtagaac ccggatcccc attcaaaacc ggctacttta gtcagtctgg
      301 gcattatttc gagatgccag tgaaaatagt cggtacagtg ttcgtcgcat ggggcggtat
      361 ggatgataaa gttgaatggc gggttattga gtgtttttat cagcttgcga agggttgcgt
      421 gcatgatgcc tgccaaccct tcgatttcat tgctgtcgat gtcgccgaat gagtctttgt
      481 gatctttggg agcgatccag gtttcaaagg gaaaccgtga tgcaaacggt gcaaaagcga
      541 cgaactgttc gttctcgtga atgatgcggt cgccagaact cagttcctgg cgaataatgt
      601 cgcagaaaac gcagcgttct ttgtagtagt aatagcgctt ggctccttca acctcttcca
      661 ttgcacgttt tggaatgatc ggagtggcga tcagctgaga atgagggtga tcgaggctgg
      721 ctccggccgc agaaccctga tttttgaaga ttagaatgta tctgaatctt ctatcgcgcc
      781 tgagatctcg atatcgatca gtatatgtac ggagaattct ggagatctgc tcgacagtgt
      841 aattgtgaaa cccgtcgtcg tgattcggtg attcaataat gacttcgtgg gcgcccacgc
      901 cattcatcat gtcgtagacc ccgatcccgg ttcgatcaag atcaccctcg atctgcaggg
      961 cgggaaactt gttcggtatg actctgactg cccaacccgg ctgatttgga agggtttccg
     1021 gggtgcggta tgacatgact tccggtggag ccatgtcttc gtggccaggg cagaagggac
     1081 atttgttctg ctggcgtggg ggctgtggaa tctggaaatc tgcaggggtt ttagcctgct
     1141 catgcaggat gatgacccaa cgcttggtta ccgggtcttt gcgtagctga ggcatgttga
     1201 tgttcccttc cgcttcattt ttctggtggg attatacaat aagaacaacg gtatgcgcaa
     1261 tttgttcatg attctgcacg acgttcaaag ctgtgtacta tgttcggtca gttctgtaca
     1321 gtcgtgacga aaaaggttga caagttgttg agccttggtt aatctattgc agtcatcag
//
LOCUS       DAORHD010001461         1378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_133513_length_1378_cov_4.495843, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001461 DAORHD010000000
VERSION     DAORHD010001461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133513_length_1378_cov_4.495843"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="PKO06_22630"
     CDS             complement(<1..1038)
                     /locus_tag="PKO06_22630"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV72523.1"
                     /translation="MIGTVINGKYEIRQSLSESHQFEVFSALEVETGTTVVLKLMLAE
                     LAERTDRVRLFADEVAQFARVDHPGVARVLDLDMTGSRPFVVTEFVKGTDLLSWVQNG
                     PVSFNEATKALQGIATVLQAAYDQGILCRSVKLSNVLRTAKGEIKVLSFSLPRLKLVG
                     REDADPAASIQSDLYFLGTAYYELLTGESAIRRRGGLNECWDEKLRQAMRIRHPHLNP
                     EQVDRLVALIDSTLTRDINRRCKDHAAFLVGLADLLHLSESSSRQNKATGKKQFASAA
                     EVVDAIQGRMLATGTGDAVHFSSAQPAPMTGGRMADNVVALNPSAGAQVATLSLNQTL
                     VRGDLSVGVSSD"
     gene            complement(1054..>1378)
                     /locus_tag="PKO06_22635"
     CDS             complement(1054..>1378)
                     /locus_tag="PKO06_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72524.1"
                     /translation="ILPPESPDADLPDALADVPATDEEEEDPETPADVTKPTGPQALV
                     TFVMPDGFMQKEVKFILLSPDGRQEVYSGVHKPLDLVKVKVPKSGQGKIQIYINNIFI
                     EERSL"
BASE COUNT          287 a          365 c          406 g          320 t
ORIGIN      
        1 gtctgacgag acgccgactg agagatcgcc tctgaccaga gtctggttca gactgagcgt
       61 cgcgacctga gcgccagcag acggattcag agcgacgaca ttgtcagcca ttcttccccc
      121 ggtcatgggt gcgggttgag cagatgagaa atgaactgcg tcaccagtac cggttgccag
      181 catgcggccc tggatagcat caaccacctc tgccgctgac gcgaactgct ttttccctgt
      241 cgccttgttc tgtcgactac tgctttcact gaggtgaagc aggtcggcga gcccgacgag
      301 aaatgccgca tgatccttgc aacggcggtt gatgtcgcga gtcagggtcg agtcaatcaa
      361 ggcgaccagg cgatcaacct gctcgggatt cagatgcggg tgacgaatac gcatggcttg
      421 ccggagtttt tcatcccagc attcattcag tccgccgcgc cggcgaatgg ctgattcacc
      481 agtgagaagc tcatagtaag cggttcccag aaaatagaga tcggactgaa tactcgcagc
      541 tgggtcggcg tcttctcgtc cgacgagttt cagacggggg agagaaaagg aaagaacctt
      601 gatctctcct ttggcggtgc gcaggacatt ggacagcttg accgagcggc agagaattcc
      661 ctggtcgtat gcagcctgca gaacggtcgc gattccctgc agggctttcg tcgcttcatt
      721 gaacgagact ggtccgtttt gtacccagga aagcagatcg gtgcctttga cgaactcggt
      781 gaccacgaac ggtcgcgagc ctgtcatgtc gagatcgagt actcgagcaa ctcccggatg
      841 gtcgacccgg gcgaactgtg cgacttcatc ggcgaacagc ctgacacgat cggttcgttc
      901 ggccagttct gcaagcatga gtttgaggac aaccgtcgtg ccggtttcga cttcgagggc
      961 ggagaacacc tcgaactggt ggctctcgga gagcgattgg cgaatctcgt atttcccatt
     1021 gatgacagta ccgatcatgt gtgcctccga tgcttacaga ctgcgttctt cgatgaagat
     1081 gttattgatg tagatctgaa ttttgccctg gccggatttc ggaaccttga ctttcaccag
     1141 atcgagcggt ttgtgaactc cggaatagac ttcctgacgc ccatcgggag acagcagaat
     1201 gaacttcact tctttctgca tgaacccatc gggcatgacg aacgtgacga gcgcttgtgg
     1261 tcctgtgggt tttgtgacgt cggcgggagt ctctggatct tcttcttcct cgtcggtggc
     1321 tggtacatct gccaatgcgt cgggaagatc cgcatcgggt gattccggtg gcagaatc
//
LOCUS       DAORHD010001462         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_134210_length_1374_cov_3.229719, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001462 DAORHD010000000
VERSION     DAORHD010001462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134210_length_1374_cov_3.229719"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKO06_22640"
     CDS             <1..773
                     /locus_tag="PKO06_22640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72525.1"
                     /translation="KHLRFSKKKGGHLLIFLPTDALQEKPIASLSDETLEFLEKRLEE
                     NPDIPTIVFAHAPLEGSYIDADDEEGLPEVHSTAQPAKRIKAILKKHHQVFLWVAGHR
                     HTKPGTRNYFHETNQVGKVTCIHVPNIAPDKGWVLTLAVSPEQALVRTWDLQKSKFIE
                     KYNRRFVHKQKTDVKPEEDASNSDKPADKPADKPIVTEPSTGPTSEPETTTTGTSTVS
                     DGEKSFDAQDLMDWFQKGWKKFTAFVDRFFNALKKFFS"
     gene            complement(796..>1374)
                     /locus_tag="PKO06_22645"
     CDS             complement(796..>1374)
                     /locus_tag="PKO06_22645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005000121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNV72526.1"
                     /translation="AKAFCRCYDFLASLLPYSNAAWEKLSIFLNYLIPKLPTPREEDL
                     TKGILELIDMETYRVEKKAAQAIKLPDQDTKILPSPVDDGGKGFPKNTDKLSNILKSF
                     NEQFGRLFKDSDRVFKRIREDIAPRVAADEAYQNAKKHTPGAAKLEHDKALQSVMLTL
                     LKDDTEVYKHFMQNEAFKQFVTDLVYQLTEAA"
BASE COUNT          316 a          362 c          390 g          306 t
ORIGIN      
        1 agaagcatct gcgcttttcg aagaagaagg gcgggcacct gttgatcttc ctgccgaccg
       61 atgccttgca ggagaagccg attgcctcgc tttctgacga gacgctggag tttctggaaa
      121 agcggctcga ggaaaacccg gatatcccga ccatcgtgtt tgcccatgcg ccgctcgaag
      181 gcagttatat cgatgctgac gatgaagaag gactgccgga ggttcattcg accgcgcagc
      241 ctgccaagcg catcaaagcc attctgaaga agcaccatca ggtgttcctg tgggtcgcgg
      301 gacatcgtca cacgaaacct ggcaccagga attactttca cgaaacgaac caggtgggca
      361 aagtcacctg cattcacgtg ccgaatatcg ccccggacaa aggctgggtg ctgacgctgg
      421 cggtcagccc cgaacaagcg ctggtgcgca cctgggatct tcagaaaagc aaattcattg
      481 aaaagtacaa ccgccgattt gtgcacaagc aaaagaccga tgtgaagcct gaagaggatg
      541 cttcgaactc ggacaagccc gcggataaac ctgcggacaa gccgatagtg accgaaccgt
      601 ctaccggacc aacgtccgag ccagaaacga ccacgaccgg cacgtcgacc gtgtctgacg
      661 gcgaaaagtc ttttgacgct caggatctga tggactggtt ccagaaaggc tggaagaagt
      721 tcacggcttt cgtcgaccgc ttcttcaacg ccctgaaaaa gttcttcagc tgaccgcttt
      781 caccctgccg atacgttatg cagcctcagt cagctggtac acgaggtcgg tgacgaactg
      841 cttgaacgct tcattttgca tgaagtgttt gtagacctcg gtgtcgtctt tcaagagggt
      901 gagcatgacg gactgcaggg ccttgtcgtg ttccagtttg gccgcgcccg gggtgtgttt
      961 tttcgcgttc tgatacgctt cgtcggcggc gacccgcggg gcgatatctt cgcgaatgcg
     1021 cttgaagaca cggtccgaat ccttgaagag ccggccgaac tgttcgttga agctcttcag
     1081 gatgtttgac agtttgtccg tattcttcgg aaagcctttt cccccgtcat cgacgggaga
     1141 agggaggatc ttcgtgtcct gatcaggcag tttgatggct tgagccgctt tcttttccac
     1201 ccggtaggtt tccatgtcga tgagttcgag aatccctttg gtcagatcct cttcgcgggg
     1261 cgtcggcagt ttcggaatca gataattcag aaaaatcgaa agcttctccc aggcggcgtt
     1321 ggaatagggt agcagagagg ccagaaaatc atagcatcga cagaaggctt tggc
//
LOCUS       DAORHD010001463         1373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_134398_length_1373_cov_2.669196, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001463 DAORHD010000000
VERSION     DAORHD010001463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134398_length_1373_cov_2.669196"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="PKO06_22650"
     CDS             <1..1213
                     /locus_tag="PKO06_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNV72527.1"
                     /translation="DIDGRVYPAKLAGKLYPKGIPIRAESELLDLIRKEKVDEVIFSY
                     SDVNHEYIMHHASRITAAGASFKLLGYQQTAIKAKVPVVSVCAVRTGAGKSQTTRRVV
                     QTLKDMGLKVVSIRHPMPYGNLEAQICQRFASYKDLDHHKCTVEEREEYEPHIDMGAV
                     IYSGVDYQKIGEAAEKEADVIVWDGGNNDFPFFQSDLKIVVADPMRPGHESSYYPGEA
                     NVYTADVIVINKIDSCYPDDLDTLRDNLYHMNPKAVVVHGASPIFLDQGEKIRGKRVL
                     VIEDGPTLTHGEMQIGAGYVAARRYGAGEIVDPKEYFVGTLLQALQKYPDIGPILPAL
                     GYGKKQVADLEKTINSVPCDLIISATPIDITRVVKVKKPMLRVRYELQEIGEPTLAEI
                     IKKQLKVKKGK"
BASE COUNT          309 a          392 c          388 g          284 t
ORIGIN      
        1 cgatatcgat gggcgtgtgt atccggcaaa actggccggc aaactgtatc cgaagggcat
       61 tcccattcgc gcggaatccg aactcctcga cctgatccgc aaggagaaag tcgatgaagt
      121 cattttttcc tacagcgatg tgaatcacga atatatcatg caccacgcga gccgcatcac
      181 cgccgcgggt gcctcgttca agctgctggg gtaccagcag accgccatca aggcgaaggt
      241 cccggtcgtt tccgtgtgcg ccgttcgcac cggggccggc aagtcccaga ccacccgccg
      301 cgtcgtgcag acgttgaagg atatgggtct caaggtcgtg tcgattcggc atccgatgcc
      361 ctacggcaat cttgaggcgc agatctgcca gcgtttcgcc tcgtacaaag acctcgacca
      421 tcacaagtgc acggtcgaag agcgcgaaga gtacgaaccg catatcgata tgggcgcggt
      481 catctattcc ggcgtcgact atcagaagat cggcgaagcc gcggaaaaag aagccgatgt
      541 catcgtctgg gacggcggaa acaatgattt tccgttcttc cagtcagatc tcaagatcgt
      601 cgttgccgat ccgatgcgcc cgggccacga atcgtcctat tatcccggtg aagcaaacgt
      661 ctataccgct gatgtcattg tgatcaacaa gatcgattcc tgctacccgg acgatctcga
      721 cacgctgcgc gataacctgt atcacatgaa tccgaaggcg gtcgtggtgc atggcgcttc
      781 gccgatcttc ctcgatcagg gcgaaaagat ccgcggcaaa cgcgtgctgg tcattgaaga
      841 tggtccgacg ctgactcacg gcgaaatgca gattggtgcc gggtatgtcg ccgcccgccg
      901 ctatggcgcc ggtgagatcg tcgatccgaa ggaatatttc gtgggcacgc tgctgcaggc
      961 gttgcagaag tatccggata tcggtcccat cctgccggcc ctcgggtacg gcaagaaaca
     1021 ggtcgccgat ctcgagaaga ccatcaattc cgttccgtgc gatctgatca tctcggcgac
     1081 tccgattgac atcacccgcg tggtcaaggt gaagaagccg atgctgcgcg tgcgctacga
     1141 actgcaggaa atcggcgaac cgacgttggc cgagatcatc aagaagcagc tgaaggtcaa
     1201 gaaagggaag taatcctgtc ggatacgatc tgaacgggcg gccgactggc cgcccgttct
     1261 ctttcaagct gggttcgccc aagagctcga gatgatgaac cgccaaaggt tgatgaacat
     1321 tgctttcctg gtttgactct cttgtaccca ttttcgaaca tggcccctac ctt
//
LOCUS       DAORHD010001464         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_134839_length_1370_cov_4.091255, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001464 DAORHD010000000
VERSION     DAORHD010001464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134839_length_1370_cov_4.091255"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="PKO06_22655"
     CDS             <1..1126
                     /locus_tag="PKO06_22655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72528.1"
                     /translation="IVFGGPSTSNFADHLLAYGDAVLKGRGEVTLPLWLTAVAQNTDP
                     TVIPGIITKCSGKLFRNAQPVPTADAPTRYDLVEGFGPRTRRDGLFGMPKEAVYSLFA
                     STGCVRHCRFCITDRVWNHRSHDSVTRDLEQVLHLHQHRGTARFMLVDDCLFGNLDWT
                     KELLRRIAHTCQGHDVSFSTQFHVQPTADDELMRLFREARFTSLALGFETTSQTTLNH
                     ERKGTTTTQNDQAIEQCRRFGIVPYGYFVVGFDSDDELTVRDVFQYIYDRRLMAQVLP
                     VGLMSRDEDGNPTPDAGRILSETSFGATVFVSHRPARLSASQLQMLINRGYQRISSLR
                     RLGHFSTAYERRFLIGLNRCLAIWHPLMQHHVTWLQNHGV"
     gene            1131..>1370
                     /locus_tag="PKO06_22660"
     CDS             1131..>1370
                     /locus_tag="PKO06_22660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72529.1"
                     /translation="MTISLETLFFQNIFVFLFGSFFLWFYQSYNKVYPGFGFWTAGSL
                     CASASFLCSLLRPFTPLWLSVLFVQSTSILSVLLRF"
BASE COUNT          256 a          418 c          360 g          336 t
ORIGIN      
        1 gattgtgttc ggcggacctt cgacatcaaa ctttgccgac cacctgctgg catacggtga
       61 tgccgtgctg aaggggcgcg gagaggtcac cctgccgctc tggctgaccg cggtcgcgca
      121 gaatacagat ccgaccgtca ttcctggcat catcacgaag tgttcaggaa agctctttcg
      181 caacgcacag ccggtaccca cggcagatgc gcccacgcga tacgatctgg tcgaaggttt
      241 tggaccgcgc accaggcgag atggtctctt cggcatgccc aaggaagccg tgtattcact
      301 gtttgcctcg accggttgcg ttcgacactg ccgtttctgc atcaccgacc gcgtgtggaa
      361 ccatcgttct catgacagcg tcacacgcga tctcgaacag gtgttgcacc tgcatcagca
      421 tcgcggcacc gcgcggttta tgctggtcga cgactgtctg ttcggaaatc tcgattggac
      481 caaggaactg ttgcgccgaa tcgcccatac ctgtcagggg catgacgtca gcttttcgac
      541 ccagtttcac gtgcagccga cagccgatga tgagttgatg cgactgtttc gcgaggcgcg
      601 gttcaccagt ctcgccttgg gtttcgaaac gacttcacag acgacgttga atcacgaacg
      661 caagggaacc acgacgactc aaaacgatca ggcgatcgaa caatgccgac gattcggcat
      721 cgtcccctat ggttactttg tggttggctt cgacagcgat gacgagctca cagtgcgcga
      781 tgtcttccag tacatttacg atcgccgcct catggcccag gtgctgcccg tcggcctgat
      841 gagtcgtgat gaagacggca acccaactcc cgatgccggt cgcattcttt ccgaaaccag
      901 tttcggcgcc accgtcttcg tctcgcaccg accggcgcga ctttctgcat cacagttgca
      961 gatgttgata aatcgtggct atcaacggat ctccagcctg cggcggctgg gccatttttc
     1021 gacggcgtat gaacggcgct ttctcatcgg cctgaaccgt tgcctggcca tctggcatcc
     1081 actcatgcag catcatgtga cgtggctgca gaatcacgga gtgtgatctc atgaccatca
     1141 gtctcgaaac cctgtttttt cagaacatct tcgtttttct cttcggcagc ttctttctct
     1201 ggttctatca atcgtacaac aaagtctacc ccgggttcgg tttctggaca gcgggatcgc
     1261 tctgtgcctc cgcctctttt ctctgttcgc tcctgcgccc gttcaccccg ttgtggctga
     1321 gcgtcctgtt cgtccagtcg acttcgatac tttccgttct cctgcgcttc
//
LOCUS       DAORHD010001465         1370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_134900_length_1370_cov_2.731559, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001465 DAORHD010000000
VERSION     DAORHD010001465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134900_length_1370_cov_2.731559"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKO06_22665"
     CDS             <1..790
                     /locus_tag="PKO06_22665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72530.1"
                     /translation="RLHAHVAHQALQRVQKGDFARRLPICWGAEFDTMARSFNSMTAS
                     AEEGRILGKFVSGSTLALLSTGQGSEAKLSGTNQHVVAMFVELAGFKLVLKSRHPEWI
                     VEKVNWYFETLSRIIRRHGGEIDKFIGDKILAVFRDEENGSSTSRYQRAIEAALEMKR
                     SIQHEVHRLEMDLGVGIVSGPVLAGVMGTQSVRLEFTVIGDTVNLASRLADVAGCLPE
                     GGIVVERALVDALRESGWGGAFESLPVKTVKGKKREVEVFRLNA"
     gene            complement(920..>1370)
                     /locus_tag="PKO06_22670"
     CDS             complement(920..>1370)
                     /locus_tag="PKO06_22670"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNV72531.1"
                     /translation="YVLLVKVSFLLAQLFLVFICLVFGVVTLFKDGPYMFRFADIALE
                     AQSLGKKFMGLRVLKQDGKTQISWNESAQRNMPLAVPYFLVMLTILITAATSFMPLAL
                     QIIPFLVSILVFFICIGIIGFELFNIWKDPQGRRWGDRSAGTTVFMD"
BASE COUNT          320 a          343 c          421 g          286 t
ORIGIN      
        1 tcgcctgcat gcgcacgtgg cgcaccaggc gttgcagcgt gtgcagaagg gagacttcgc
       61 tcggcgtttg cccatctgct ggggggctga gttcgacacc atggcccggt cgttcaactc
      121 gatgaccgcc tcggccgagg aaggacgaat tctgggaaaa ttcgtttcgg gttcgacgct
      181 tgctctactc agcacgggtc aagggtctga agcgaaattg tctgggacaa atcaacatgt
      241 cgtggcgatg tttgtcgagc tggcaggttt caaactcgtt cttaaaagtc ggcatcctga
      301 atggattgtc gagaaagtga actggtactt tgaaaccctg tcgcgcatca tccggcggca
      361 tggtggagag atcgacaagt tcattggcga caaaattctg gcggtgtttc gcgatgaaga
      421 aaacgggtct agcacgtctc gatatcaacg agccattgaa gcggccctcg agatgaagcg
      481 ctcgattcaa cacgaggtgc accgtctgga aatggatctc ggggtgggca ttgtcagcgg
      541 tccggtactg gccggagtca tgggaacgca gtcggtgcgg ctcgagttca cggtcatcgg
      601 cgataccgtc aacctggcat cgcgactggc cgatgtcgcc ggttgtctgc ccgaaggtgg
      661 aatcgtcgtg gaacgggctc tggttgatgc cctgcgcgag agtggctggg ggggggcttt
      721 cgagtcgctg cccgtcaaga ccgtgaaggg aaagaagcgc gaggtcgagg tgtttcggct
      781 gaacgcgtga gtggtttgtc ctacctgata acggcgacta tcagctcttg aaaaagagta
      841 gcattcaagc attcagaaag aaaacacccc cgaacgtttt gcctgaaacg atcgagggtg
      901 ttgctggtat ctcgggaact caatccatga agacggttgt cccggctgat cgatcgcccc
      961 agcggcgccc ctggggatct ttccagatat tgaagagttc gaatccgatg atgccgatgc
     1021 agatgaagaa caccagaatg ctgacgagaa acggaatgat ctgcagagcc agaggcatga
     1081 agctcgtggc ggccgtaatg agaatcgtga gcatcaccag aaaatatggc accgccagtg
     1141 gcatgttgcg ttgtgccgac tcgttccagg agatctgggt tttgccgtcc tgtttcagca
     1201 cgcgcagacc catgaacttc ttcccgagac tctgggcttc gagagcgatg tcggcaaacc
     1261 ggaacatgta cggtccatct ttgaacaagg tcaccacgcc gaacaccagg cagataaaga
     1321 ccaggaacag ctgggccaac aggaacgaaa ccttcaccag aagaacatac
//
LOCUS       DAORHD010001466         1369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135055_length_1369_cov_2.981735, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001466 DAORHD010000000
VERSION     DAORHD010001466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135055_length_1369_cov_2.981735"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKO06_22675"
     CDS             <1..403
                     /locus_tag="PKO06_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72532.1"
                     /translation="HQVGQPSGQHREQEVTEALREFQVTSSYLRVDNGKINRGQTFWI
                     AEKQNGWARIVCLETKNSPAPLVGWVSLVELSQRSTWRGEIPAATYPPPAPVVMYPPV
                     RYEPTVIIIDDDDWHWRRRRPGHHWPHRRRW"
     gene            538..>1369
                     /locus_tag="PKO06_22680"
     CDS             538..>1369
                     /locus_tag="PKO06_22680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72533.1"
                     /translation="MKAGFQKQQRGTGGRRHGTGAGLIGALLLLCALPCLVAGMPASA
                     MKVHFSRARNMLEKDPKAASAEWARGRIVDHAKPKTPRKAATSTRDLPRSTAPTPLVR
                     GSSALDVVRMEAEALFAYAEQYGASAPVLRRARELLKLRECPKLHRMLLFASWASGRR
                     SDALFHFERADLSTLQRAIWFVKLIPVRISTAGLGDWVKFVVWLVLMVLLLGQIRSLR
                     LGTGPREQTRSPLWLENLHTQVQELIAKTVSRLNPSPALKHGGAVLTLELSSGSPGLE
                     R"
BASE COUNT          264 a          399 c          421 g          285 t
ORIGIN      
        1 ccatcaggtc ggacagccgt ccggacagca ccgcgaacag gaagtgacgg aagccctgcg
       61 tgagtttcag gtcacctctt cgtatctacg tgtcgataac ggcaagatca accgcggtca
      121 gacgttctgg atcgcggaga agcagaacgg ctgggctcgg atcgtgtgcc tggaaacgaa
      181 gaattctcct gcgcctctgg tcggttgggt ttcgctggtc gagctgtcac aacgctcgac
      241 ctggcgtggg gagatcccgg ctgcgacgta tccgccgccg gcgcctgtgg tgatgtatcc
      301 accggtacgc tatgagccaa cagtcattat tatcgatgat gacgactggc actggcgccg
      361 tcgtcgccct ggacaccact ggccgcaccg ccggcgctgg taaacaacct tattcagctc
      421 gcgcgctgga taacgcgcga gagtctctcg tctcgcgaaa gaaattgact ccgtcgtctc
      481 gctattcatg cctgtttctg ttgattgttc cccctcttcg cagtacaatg agaaaagatg
      541 aaagcgggtt ttcagaagca acagcgcggc acgggcggac ggagacacgg gacaggagcc
      601 gggttgatcg gtgcgctcct tttgctgtgc gccttgcctt gtctggttgc gggcatgccg
      661 gcttcagcca tgaaagtcca tttttcccgg gcgcgaaaca tgctcgagaa agacccgaaa
      721 gcggcatccg cggagtgggc gagggggcga atcgttgacc atgccaagcc caaaaccccc
      781 agaaaggctg cgacctcgac ccgcgatctt ccgcgttcca ctgctcccac accgctcgtg
      841 cgtgggtctt ccgcattgga tgtcgtccgc atggaagctg aggcgctgtt cgcctatgca
      901 gagcaatacg gggcgtctgc tccggtcttg cggcgagctc gagaactgct gaaacttcgc
      961 gaatgcccga agttacaccg catgctcctg ttcgcctcct gggcctccgg aagacgcagt
     1021 gacgccctgt tccactttga gcgagctgat ctgtcgacgc tgcaacgcgc gatctggttt
     1081 gtgaagttga taccggtgcg aatcagcact gccggcctcg gggactgggt gaagtttgtg
     1141 gtgtggcttg tgttgatggt gctcctgctc gggcagatca gaagtttgag actgggaacg
     1201 ggcccgagag agcagaccag gtcgcccctt tggctggaaa accttcacac tcaggtgcaa
     1261 gagctgatcg cgaagacggt gtcccggctg aatccttctc cagccctgaa gcacggagga
     1321 gccgtgctga cgctcgagct ttcctcagga agtccaggcc tcgaacgca
//
LOCUS       DAORHD010001467         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135361_length_1367_cov_3.351372, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001467 DAORHD010000000
VERSION     DAORHD010001467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135361_length_1367_cov_3.351372"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..886)
                     /locus_tag="PKO06_22685"
     CDS             complement(131..886)
                     /locus_tag="PKO06_22685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72534.1"
                     /translation="MRHTKTLLFRLLLILTGVFSVVTAWAGGSSDSCWSAYRERIEMK
                     PDGSALVKIQARLEGARPANLVIPVTVGKYGSGRLLSPQSLELPGEILGGTTVVRVDL
                     RAYPDELKDVTLEYVATGVFDPQQSRKTDYGNVVGTYKYMQLQPCMISSYTAELLLPE
                     GMQLKAVDEFLPKVKKDDPHDPYAVRQTNDGRQMIVIEVNHLAFGQQIVLKWRQYRKD
                     GQYGLFVGVLLLAVLYLVFFRDLISTTQETQKE"
     gene            complement(898..>1367)
                     /locus_tag="PKO06_22690"
     CDS             complement(898..>1367)
                     /locus_tag="PKO06_22690"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HNV72535.1"
                     /translation="TCPASRSHHHAYAGGLALDNTGAGRWAVNQVFSVLKISPGTNFW
                     LIYIGVISVMMYSHLVFTSKTMRTMILIPFIILMARQLNLSPLALALPAAFTIDWVIT
                     LPISAKPNVILFGTGQYSVLDNIKIGLLVSTIGVILLTIAGMTWFRFLGIIPY"
BASE COUNT          310 a          401 c          382 g          274 t
ORIGIN      
        1 ggcgtggggt cgaacgcaga ggatctgcgt ccgctcggcg aggcgctcac tgcgcagcgg
       61 ccgcaggcct gcgtggtcag cgtgcgctcg cccgatgcat ccggacctcc tcgttaacga
      121 gcgcatcgct tcactccttt tgcgtttcct gtgtggtgct gatcagatca cggaagaaga
      181 cgagatacag tacggcaagc agcaacaccc ccacgaacaa gccatactgt ccgtctttgc
      241 gatactgacg ccacttgagc actatctgct gaccaaaagc gagatgattc acctcgatga
      301 cgatcatctg gcggccgtca ttcgtctgcc gtacagcata cggatcatgc ggatcgtcct
      361 tcttgacttt gggcagaaac tcatcgaccg ccttgagctg cataccctct ggaagcagaa
      421 gttccgctgt atagctgcta atcatgcacg gttgaagctg catatacttg tacgttccca
      481 ccacattgcc gtaatccgtt ttgcgcgact gttgcggatc aaacacccca gtagccacgt
      541 attcaagcgt cacatccttg agttcatcag gatatgctcg cagatcaact cggacaaccg
      601 tggtccctcc aaggatttct ccgggcagtt cgaggctctg cggactcagc aatcggcctg
      661 agccatactt gcccacggtg accggtatca cgaggttcgc tggtcgtgcg ccctcgagtc
      721 gcgcctgaat tttcaccagc gccgaaccat ccggcttcat ttcgatccgt tcacgatagg
      781 cagaccaaca agagtcactt gagccaccag cccaggccgt caccacggaa aacactcccg
      841 tgagaatcag cagcaggcgg aacagaagag tcttcgtgtg tcgcatcgcc acgctcctca
      901 gtaggggatg atgccgagaa accggaacca ggtcatgccg gcgatggtca gcagaatgac
      961 gccgatggtc gagaccagca gcccgatctt gatgttgtcg agaaccgagt actgcccggt
     1021 accgaagagg atcacgttag gcttagcgct gatcggcagc gtgatgaccc agtcgatcgt
     1081 gaacgctgcc ggcagggcca gcgccagagg agagaggttc agttggcgcg ccatcagaat
     1141 gatgaacgga atgagaatca tggttcgcat ggttttgctg gtaaacacca ggtgcgaata
     1201 catcatgacg gaaatgacgc cgatgtagat gagccagaaa ttcgttcctg gggagatttt
     1261 cagcacggaa aacacctgat tgacggccca gcgacccgcc cccgtgttat cgagcgccag
     1321 cccgcccgca taggcatggt ggtgcgaccg cgaagcagga caggtcc
//
LOCUS       DAORHD010001468         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135401_length_1367_cov_2.641006, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001468 DAORHD010000000
VERSION     DAORHD010001468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135401_length_1367_cov_2.641006"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PKO06_22695"
     CDS             <1..372
                     /locus_tag="PKO06_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72536.1"
                     /translation="YEVKAVRLPYRSIAKRTKKQSPRTGKVIPAMTGKPEPRENDLSN
                     TRYLPRMVYDEVEPLPCQALHHAVRLGVPDVARSRSPPQKRNISEKGTSPTNQLYKFV
                     FRQRGFTLSRVIIEEEDARAA"
     gene            complement(755..1003)
                     /locus_tag="PKO06_22700"
     CDS             complement(755..1003)
                     /locus_tag="PKO06_22700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNV72537.1"
                     /translation="MPVETMQENGFLRLSDVLKLFPVSRATWWRGINSGKYPKGVKLG
                     ERVTAWRVSDIRELIERTQAPVTANREKLLDQTGDRQG"
     gene            complement(1007..1261)
                     /locus_tag="PKO06_22705"
     CDS             complement(1007..1261)
                     /locus_tag="PKO06_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72538.1"
                     /translation="MSKKPSSEPVETIDSFKLYDLALGIKNVADLLRMTMELFPRDES
                     LTGDARLVAGNFEQIQTVVVASVRLAEQISTDLLTACGEG"
BASE COUNT          307 a          407 c          353 g          300 t
ORIGIN      
        1 tacgaagtca aagcggttcg gttgccatac cgcagcatcg caaagcgaac gaagaagcag
       61 agcccccgaa cgggcaaggt cataccggcc atgaccggca agccagagcc gcgagaaaat
      121 gacctttcca atacgcggta tctgccccgc atggtctacg atgaagtaga gccgttgccc
      181 tgccaagcct tgcatcatgc cgttcgtctc ggtgttcctg atgtagctcg aagcagaagc
      241 ccgccacaga agcgcaacat cagcgagaaa gggacaagcc ccacgaacca attgtacaag
      301 ttcgtctttc gacaacgtgg gttcaccctc agccgggtca taatcgaaga ggaagacgcc
      361 cgggccgctt gaccagtgca tatcatcaag cgttcgtgcc acgctgccag ggtgaagcgg
      421 tttctctttc ttcgtgacta ccggcccgct cacctggtcg aatacgccaa acgtgaaacc
      481 ggttgtgctg tcggctgctt caaggtatgc cgcgaactca gagagcgttg agaacgcctt
      541 gcgttcgtgc gttcccccgc acatttcccc caggggtgac ttgcagagcc gccccccttc
      601 gaggaagatt ttctttccga tcttctgaga agatcgaaac agggtaaaag caacatcacc
      661 agagcataca cgcgcccggg ttgcctcaac agcttggttc aagggtaggg gatgattcat
      721 gatatgtcgg gccgctcacc aggcccggtc tcccctaccc ttgccggtcg ccggtctggt
      781 cgagaagctt ttcccggttg gccgtcacag gggcttgtgt gcgttctatc aactcgcgaa
      841 tgtccgagac gcgccaagcc gtgacacgct ccccgagctt cacacccttt ggatacttac
      901 cggaattgat cccgcgccac cacgtcgccc ggctcaccgg aaacagcttc aacacatcag
      961 acaaccgcag aaaaccgttt tcttgcattg tctcaaccgg catggttcag ccctccccgc
     1021 aagcggtcag caagtcggta gagatttgtt ctgccagtct gacagaagcg acaaccacgg
     1081 tttgaatctg ttcaaagtta ccagccacaa ggcgagcatc cccggtcagg ctctcatcac
     1141 gtgggaaaag ttccatcgtc attctgagca ggtcagcaac gttttttatg cctaacgcga
     1201 ggtcataaag tttgaaactg tcgatggttt caacgggttc tgatgatggt ttcttactca
     1261 tgatgcaccc ccgcgtttct tttgtgctgg tcgctttcga ggctgttttt cagccggtgc
     1321 aagaccgttg aacagcttta cttcaccccg cttgagaagt cgcaaac
//
LOCUS       DAORHD010001469         1367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135473_length_1367_cov_1.897104, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001469 DAORHD010000000
VERSION     DAORHD010001469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135473_length_1367_cov_1.897104"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PKO06_22710"
     CDS             <1..379
                     /locus_tag="PKO06_22710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72539.1"
                     /translation="CAPAIAWVFTSDPKVSEVLISYIRIISFGYGMMEVHRYCGFILN
                     GVHQPASATTLNVIRVLVLLVPLSFLGAHLWGIRGVFAARLITDLTVGSIGLYWVTRI
                     LRGVKPTAVSIDSLETSDGFPLT"
     gene            581..>1367
                     /locus_tag="PKO06_22715"
     CDS             581..>1367
                     /locus_tag="PKO06_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72540.1"
                     /translation="MSLNKRGLAIGLIVGFGVFLSLIIFAIMRFSSNEIHFTAQSAFV
                     KKATLLAFSGINVAEMALAKGRWYQQPYRPAVDLNGDGTTTRTEAEASSLIDRPNFAE
                     LEFEPDGPGEGQVRIFFQEVAKKDVSLTGNLLYARYKLQTADLLDHIKVYSVAEYRGE
                     RVMVYGKFIMSPSPLLNSPDIELASSTNLPKTLYRVMVEPPQLVKEQIAQGYTPFRNG
                     KVTGVFKKVGDTVGPNDTLFRIADDEPSHKAEGWLATQEANPKA"
BASE COUNT          279 a          397 c          399 g          292 t
ORIGIN      
        1 ctgcgccccg gcgatcgcct gggtgtttac gagtgacccg aaagtgtccg aggtcctcat
       61 ctcgtacatc cgcatcattt ccttcggata cggcatgatg gaagttcacc ggtactgcgg
      121 tttcattctg aatggcgtgc accagcctgc ctcggccacg actctcaatg tcattcgcgt
      181 gctggtcctg cttgtgcccc tgtcgtttct cggcgctcac ctgtggggca ttcgcggagt
      241 attcgccgcc aggctgatca ccgatctcac ggtcggcagc atcggcctct actgggtcac
      301 ccggatcctg cgcggcgtca aaccaaccgc tgtctccatt gactcactgg aaacctcgga
      361 cgggttccca ttgacgtaat ggtgaatcgt tgagcgattg gctggcactc agagatcatg
      421 gagcggtctg cgcgacccgg tctgcgcgac ccggtctgac gagggggagt cgaagagccc
      481 gggcccacgc ggaaaaagga tgcatttgcc ccggggtttc tgcttgcatg tgcccagggc
      541 agggtttaaa ctggtgccag agtgagagtg aggagtctgt atgtcactga ataagcgtgg
      601 tctggccatt ggtctgattg ttgggttcgg agtgtttctg tctctgatca tcttcgcgat
      661 tatgcggttt tccagcaacg agattcattt taccgcgcag agcgccttcg tcaagaaagc
      721 aacactgctg gctttttccg gcatcaatgt cgccgaaatg gcactggcga aggggcgctg
      781 gtatcaacag ccgtaccgac cggcggtcga cctcaacggt gacggaacga caacgagaac
      841 ggaagccgaa gcctcttctc tcatcgaccg accgaacttc gccgaactcg agtttgaacc
      901 tgacggcccg ggagaaggcc aggtgcgcat cttctttcaa gaggtcgcga agaaagatgt
      961 gtcactgacc ggcaacctgc tctatgcacg gtacaaactg cagaccgccg atctgctcga
     1021 tcacatcaag gtctactcgg tcgccgagta tcgcggggaa cgggtcatgg tctacggcaa
     1081 gttcatcatg agtccctcac cgctgctcaa ttcgcccgat atcgaactgg catcgtcgac
     1141 caacctgccg aaaacgctgt atcgggtcat ggtagaaccg ccccagctcg tgaaagaaca
     1201 gatcgcgcag gggtacacac cgtttcggaa tggcaaggtg accggcgtct tcaagaaggt
     1261 cggcgatacc gtcggtccga acgataccct gttccgcatc gctgatgacg aaccttcgca
     1321 caaggccgag gggtggctgg ccacccagga agccaacccg aaggctg
//
LOCUS       DAORHD010001470         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135601_length_1366_cov_2.223494, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001470 DAORHD010000000
VERSION     DAORHD010001470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135601_length_1366_cov_2.223494"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1160
                     /locus_tag="PKO06_22720"
     CDS             270..1160
                     /locus_tag="PKO06_22720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72541.1"
                     /translation="MKAIRLFTFFLLVAISGHAASIGVIDLDVALSFHPLMSTFDFHH
                     GRFLRVTPGTPEAERQQRILSIRETARHQEPALDAQIASLNQTLSALEQQRSLLMGMT
                     TASETASVHARELDLHRLAQKAEQYREQRDDLALVRQYPEMTSASETLAMINRIEQEV
                     HAALIETAQAEQLEVILNAADIDRHTPVGSPERDQPKRSFLESDLYNRFMGQPTPQDP
                     LDRAASQQLPWRWLQSSRYHRTLPPHWRSYPLVLHGGKDVTVTVVERVFRQYACAPEA
                     VRLILEEIRQWRGDQNRDRK"
BASE COUNT          277 a          421 c          351 g          317 t
ORIGIN      
        1 catgacgatc tgacggcagc tatccgcaca tgcccgccac ccgttttgaa tctcttgccg
       61 cgcacccttc ttcctgaaag ctttttcgat gggcccaacc cgtactctca gtttttctct
      121 actcgtgact caggttggct gaagcagtat catgccagac gtaccgccat ccgcgcgctc
      181 tttcccttcg cccgccatcc ggtggttttt ccttcgaacc ctcccacgat cgcccccact
      241 atcgcctcag ccacggagca ttctgcacca tgaaagccat tcgactgttc acgttctttc
      301 ttctggtggc catttccggt catgcggctt cgattggcgt catcgacctg gatgtcgcac
      361 tcagttttca cccgctgatg tcgaccttcg acttccatca cggcaggttt cttcgtgtga
      421 ctcccggcac ccctgaagcc gagcgccagc agcgcattct ctcgatacgc gagaccgccc
      481 ggcaccagga accagcactc gacgctcaga ttgccagcct gaaccagacc ttgtcagctt
      541 tggaacagca gcgttctctc ctgatgggaa tgaccacggc aagcgagact gcttccgtac
      601 atgcccgtga gttggacctg catcggctgg ctcagaaagc ggagcagtat cgggaacagc
      661 gtgatgatct ggctttggtg cgacagtatc ccgaaatgac aagcgccagc gagacactgg
      721 ccatgatcaa tcgcatcgag caggaagttc acgcggccct tatcgagacc gcccaagccg
      781 aacaactcga agtgatcctg aacgccgccg acatcgaccg tcacactccc gttggcagcc
      841 cggaaagaga tcagccaaag cgatcttttc tcgaatctga cttgtacaat cgttttatgg
      901 gacaaccgac tccgcaagac ccgcttgacc gggcggcgtc tcaacaactc ccctggcggt
      961 ggttgcagtc gtcccggtat catcgcacgc tgcccccgca ttggcgctcc tacccgctcg
     1021 tacttcacgg ggggaaggat gtcaccgtga cggtggtcga gcgggtgttt cgccagtatg
     1081 cctgcgctcc cgaggcggtg cgtctgatcc ttgaggagat tcgtcagtgg cggggtgacc
     1141 agaatcgtga cagaaaatga cctgacgtaa aaatttcttt acgagcgttc tcactggtcc
     1201 tcttctagag ggagtcttga gaaggaatta ttcttgcttg ctgggatact atggtagact
     1261 ggattatgaa gtcgtcaatc tcgacaacgg ggctgtctga agtcaccggt tcaaggttcg
     1321 ccctgagctt ggcgagattg acgacttcat aatccagtct accata
//
LOCUS       DAORHD010001471         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_135614_length_1366_cov_2.077803, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001471 DAORHD010000000
VERSION     DAORHD010001471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135614_length_1366_cov_2.077803"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1348
                     /locus_tag="PKO06_22725"
     CDS             173..1348
                     /locus_tag="PKO06_22725"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV72542.1"
                     /translation="MAEEAKKQEKRRPISDGPGEQKRPILSYLNPFYNMISLSEILIF
                     TKYFSTLIKAGIPVLRCLSTLSRQMSTWRFKNTIWTMRNEVEGGIPLHATFKKNEDIF
                     GLLYVNLVRVGEESGRLFNCLDRLGHLLDRELKLRRKVLSAMTYPAIISIVAIGVVTF
                     LMVVVIPQFSKLFSQFGQELPYPTQVVINISNFIKDNILNLIAGSIGFLLVLYQVNQT
                     EGGRNFFDNLKLKIPLFGTLTQKYNIALFARNLSTLFQSGVPIISGMKISIEVVENVV
                     IAQALQKVVREVEGGTPIARALERVEIMPELSTQMIEIGEESGNLDEMLERVAEFYED
                     EINFLIDQMAALIEPAFIVVLGTIVGGIVLAMYLPIFRMARVVSGGGGGGMAGGGAP"
BASE COUNT          312 a          374 c          361 g          319 t
ORIGIN      
        1 tcggcatcgt agtggcagcc acgtcaagct tttcgggagt ctctcccaat ccgtatctgg
       61 ccacgcaggt caccacggtg tatctcaatc gcggcagcgt tggcaatggt cagagtttcc
      121 gcgatatcaa gctttccgga ccatacgcgt cctggtagag aaaggaaccg ccatggcaga
      181 agaggcaaaa aaacaggaga aacggcgccc catcagcgac ggaccagggg aacagaaacg
      241 tccgatcctg tcgtatctga accctttcta caacatgatt tcactcagtg agatcctgat
      301 ctttaccaag tatttttcga ccctgatcaa ggctggtatt ccagtgctgc gctgtctcag
      361 cacgctttca cgccagatga gcacctggcg attcaaaaac acgatctgga ccatgcgcaa
      421 tgaagtcgaa gggggtattc ctctgcatgc caccttcaaa aagaatgaag acatctttgg
      481 ccttctctac gtgaatctgg tgcgcgtcgg tgaagagagc ggccggctgt tcaactgtct
      541 tgatcgtctc gggcacctgc tcgaccgtga attgaaactg cgacgaaaag tgctctccgc
      601 gatgacgtat cccgcaatca tcagcattgt tgccatcggg gtcgtgacgt ttctgatggt
      661 cgtggtcatt cctcagttca gcaaactgtt ctcgcagttc gggcaggaac tgccctaccc
      721 gactcaggtt gtcatcaaca tcagtaactt catcaaggac aacatcctca atctgattgc
      781 aggttccatc ggttttcttc tggtgctgta ccaagtcaac cagaccgaag gaggccgcaa
      841 cttctttgat aatctgaaac tgaagattcc ccttttcggc actctgacgc agaaatacaa
      901 catcgccctc tttgctcgca acctgtcaac cctcttccaa tcaggtgttc cgatcatcag
      961 cggcatgaag atcagtatcg aagtcgtcga gaacgtggtc atcgcccagg ctctccagaa
     1021 ggtcgtccgc gaggttgaag gcggcacgcc cattgccaga gcactcgagc gagtcgagat
     1081 catgccggaa ctttccacgc agatgatcga aattggtgaa gagtccggaa acctcgatga
     1141 aatgctggaa cgggtcgccg agttttatga agacgagatc aactttctga tcgatcagat
     1201 ggcggccttg atcgagccgg ctttcatcgt ggttctcggg accatcgtcg gtggaatcgt
     1261 tctcgccatg tatctgccga ttttccgcat ggcccgggtt gtctccggcg gcgggggtgg
     1321 cggaatggcc ggtggtggtg cgccgtaagc gaagtccggc cgtcca
//
LOCUS       DAORHD010001472         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_136033_length_1363_cov_2.916667, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001472 DAORHD010000000
VERSION     DAORHD010001472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136033_length_1363_cov_2.916667"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1363
                     /locus_tag="PKO06_22730"
     CDS             <1..>1363
                     /locus_tag="PKO06_22730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV72543.1"
                     /translation="SSATEKSEKRGLYQTLLSRQLELVIRERGSLITLNIGDDESISF
                     IDLAVNASGRAEHCFFLMHQKTQMEAQFLHRMSRRLSGERAPLRLFARPKVAGAGLRS
                     FPQGFDRHPELTRVAESALQTDGVVHRQVPFDGVPLILSTLPGREVSDYVLILGTPLQ
                     RILDHSRRLTQRFLAISFLMIVFALYLGWLFSSTLLHPIHLISAGIDDLTHMRYQSSV
                     SIRTGDELETIGQGLNTVLAEFHEVAMARTVQEHLLPAIGVQAGRVYCQGWSRSSAEI
                     GGEIYDYFECAPGRWVILLADSTAYGISAAILMAMTKMAVRLLAEEPQVDSARLVTRL
                     HDFYRLHVPDLQCLYLFLGLFEPASGNLQYTATSGLQVFFQPAGTAGNLQPCGVTAMG
                     AGSAGDLRTHEFRLTPGSRLLVASDGLPDAFSTWCERGRDQPATEIGPWLFQRADRHF
                     ASIA"
BASE COUNT          249 a          403 c          400 g          311 t
ORIGIN      
        1 aagcagcgcg acggaaaaga gcgagaaacg tggtctgtat caaactctgc tgagtcggca
       61 gctcgagctg gtcattcgcg agcgtggttc actgatcacg ctgaacatcg gtgacgacga
      121 gagtatctcg tttattgatc tggcggtcaa cgccagtggt cgtgccgagc actgcttctt
      181 tctcatgcat caaaagactc agatggaggc gcagtttctg catcggatgt ccaggcgctt
      241 gtccggagag cgagcaccgt tgcgcctgtt cgcccgcccg aaggtggccg gagcaggcct
      301 gcgttcgttt ccacagggat ttgatcggca cccggagctc acccgagtcg cggaatcagc
      361 gctgcagaca gatggtgttg ttcaccggca ggtaccgttc gacggggttc cgctgattct
      421 ttcgaccctg cctggtcgcg aagtctcgga ttacgtcttg attctcggca caccattgca
      481 gcgaattctc gaccactctc gccgactcac ccaacgattt ctcgccattt cgttcctgat
      541 gattgtcttc gcgctctatc tgggctggtt gttctccagt actctgctgc atccgattca
      601 cctgatttcg gcgggaatcg acgacctgac ccacatgcgc taccagagca gcgtctctat
      661 tcgcaccgga gacgaactgg aaacgattgg ccaagggttg aacacggttc tggccgagtt
      721 tcatgaggtc gccatggcaa ggacggtgca agagcatctt ctgcccgcca tcggcgtaca
      781 ggccgggcgt gtctactgcc agggttggag ccgttcttcg gcagaaattg gcggagaaat
      841 ctacgattat ttcgagtgcg ctcccgggcg ctgggtgatt ctgctggcag acagtaccgc
      901 ttatggcatc agtgcggcga ttctgatggc catgaccaag atggccgtgc ggctgctcgc
      961 cgaggaacca caggtcgaca gcgctcgcct ggttactcga ctgcacgatt tttatcgact
     1021 tcatgttcct gatctgcagt gtctgtatct gttcctgggc ctcttcgaac cggccagcgg
     1081 gaacctccag tatacggcca cgagtggatt acaggtcttt tttcaacccg ccggcaccgc
     1141 cggcaacctg caaccctgcg gtgtaaccgc gatgggagct ggctctgccg gagatctgcg
     1201 gacacacgag tttcgactga ccccgggaag tcgcctgctc gtcgcctcgg acggactccc
     1261 agacgccttc tccacgtggt gtgaacgcgg gcgcgatcag ccggcgactg agatcgggcc
     1321 ctggctgttt caacgtgcgg atcgccactt tgcctcgatc gcc
//
LOCUS       DAORHD010001473         1363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_136044_length_1363_cov_2.740061, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001473 DAORHD010000000
VERSION     DAORHD010001473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136044_length_1363_cov_2.740061"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="PKO06_22735"
     CDS             <1..1203
                     /locus_tag="PKO06_22735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72544.1"
                     /translation="EGQDSSPVPASNAVLCGEVLPDYSFENLQRIIVEQGLSEAKRLI
                     GQEFLGFNVAVELRGSGSVGVAPDWGVLAEICGFTKRVIAAATIGSPEKAFDNSGTPS
                     GLTVASGGAFTGTRPARYHVKVSTPGASGVAKVDITCLDDATQNAADVTVTTATPIDL
                     GDEGATITFTFASGSLAEDDAWFVYCYTPCVAYASRAPSDAAQSGTVYHYLGEHLFKA
                     VGVRGDVSLAFPAGAIATAQFNLQGLLSDIIDDTTPAGDFFADREIPAVVQSAGVLLG
                     LYDGAVIPELGFTSGNRLVKRSDVNSPRGIKSFRHAKRDPGWSATVEATLEATHPFWG
                     DLRARKEWPLNASVGSVAGNIVQVFVRRASSAENRMQNTDNVLMYGLSGQAHQTPGKY
                     DNIEIIVR"
BASE COUNT          256 a          431 c          424 g          252 t
ORIGIN      
        1 gaagggcagg attcaagccc ggttccggcg tcgaatgccg ttctgtgcgg tgaagtgctg
       61 ccggattatt cgttcgagaa cctgcagcgc atcatcgtcg aacagggcct ttccgaagcc
      121 aagcggctga tcggccagga gtttctcggc ttcaacgttg ccgtcgaact gcgcgggtcg
      181 ggctcggtcg gggttgcccc cgactggggc gtgctggctg agatctgcgg gttcaccaag
      241 cgcgtgatcg ccgccgcgac catcggctcg cccgagaaag ccttcgacaa ctccggcact
      301 ccgtcgggtc tgaccgtcgc cagcggcggg gcgttcaccg gcacccgccc ggcccggtat
      361 cacgtcaagg tctcgacacc tggtgcgtcc ggagtcgcga aggtcgatat cacctgtctc
      421 gacgatgcga cgcagaatgc cgctgatgtg accgtaacca cggcaacgcc gatcgacctg
      481 ggcgatgaag gcgcgaccat cactttcacc tttgcctcgg gtagtctggc ggaagacgac
      541 gcctggttcg tttactgcta caccccctgt gtggcgtatg cctcacgggc tccgtctgat
      601 gcggctcaga gcggcaccgt ctatcactac ctcggcgagc atctgttcaa ggccgtcggc
      661 gtgcgcggcg acgtcagcct ggcgtttccg gccggagcga tcgccacggc gcagttcaac
      721 ctgcaggggc tgttgagcga catcatcgac gatacgactc ctgccggcga tttcttcgcc
      781 gatcgtgaga ttcccgctgt cgtgcaaagc gccggggttc tgctcggtct gtatgacgga
      841 gcagtcattc ccgagctcgg gttcacgtct ggcaaccgtc tggtcaagcg ctctgacgtc
      901 aacagcccgc gcggcatcaa gagctttcgg catgccaagc gtgatccggg gtggagcgcg
      961 acggtcgagg cgaccctcga agcaacgcac ccgttctggg gcgatctgcg tgcccgcaag
     1021 gaatggccgc tgaatgcctc ggtcggcagt gtcgccggga acatcgtgca ggtcttcgtg
     1081 cgccgggcct cgtcggcgga aaaccgcatg cagaacaccg acaacgtctt gatgtatggc
     1141 ctgtcgggcc aggcacatca gactcccggc aaatacgaca acatcgaaat catcgtcaga
     1201 taaggagcaa cacccatgac ccctcaatcg aacgaccagt ggatcttctc gaccctgcgg
     1261 aacggcctgt tcgtgctggt agacggcaaa ccgtacgccc tgaagtgcac caaacaccag
     1321 atggacagct acacggcgta tctgaagacc aaggaagacg agc
//
LOCUS       DAORHD010001474         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_136375_length_1361_cov_3.241194, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001474 DAORHD010000000
VERSION     DAORHD010001474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136375_length_1361_cov_3.241194"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="PKO06_22740"
     CDS             <1..897
                     /locus_tag="PKO06_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72545.1"
                     /translation="STGAEASMFVSSLVGGTARALVAGATHVYTLHYLGASWRISAFA
                     QSNLAETFVVVGASTDEVRVCHSPSDNYVYGLVVSPGFATANLYRYDADLTSPTPIRN
                     LAPDLAGFDITDFAMSITPDGKTLGLTSCSNSTVDSVAHAFFNPTNGALIQKSISDLD
                     TGAAFTTRFGLSADFANNFHYLVQTNVNTVWWHKISAFGAYGAYQALPVESTAHELAF
                     DHEGRTWLLRQRPAGTPNSLIVMDQSAGIIDSNSFVEDDFPIGTPHTNLAYDVVTKKI
                     VVGLRSASNQLQLYTFDTSAKF"
     gene            1019..>1361
                     /locus_tag="PKO06_22745"
     CDS             1019..>1361
                     /locus_tag="PKO06_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72546.1"
                     /translation="MEKTFRFGLILVVALMICHTSFGAAAAERWFGMIPTAKIKEVRS
                     TLSGANVSYTTFGTLTAAIGGERLLTWLKKHPGSGEPVQLAASEKLWLMTTKDPELQT
                     VTFPGVRQLWKG"
BASE COUNT          293 a          377 c          362 g          329 t
ORIGIN      
        1 tccaccggtg ctgaggcttc catgttcgtc agttctctcg ttggcggaac agcgagagct
       61 ttggttgccg gagcaaccca tgtgtatacc cttcattatt taggtgcaag ctggcgcatc
      121 agcgcatttg cgcagtccaa tctggcggaa acttttgtcg ttgttggcgc ttctaccgac
      181 gaggttcgcg tgtgtcactc gccgagtgac aactacgtgt atggacttgt tgtctcgcca
      241 ggtttcgcca ccgcaaatct gtatcgatat gacgccgatc tcacgagccc aacgcccatt
      301 cgcaaccttg ctcctgatct ggcaggcttt gacatcactg atttcgccat gagtatcacc
      361 cctgatggca agaccctcgg tttgacatcg tgcagtaatt cgaccgtcga ctcggtcgca
      421 cacgcttttt tcaatcccac gaacggggcg ctcattcaga agagtatttc cgacctcgac
      481 accggagccg ctttcacaac aaggtttgga ttgtccgctg acttcgcaaa caactttcac
      541 tatctggtgc agacgaatgt caataccgtt tggtggcaca aaatctcagc cttcggagcc
      601 tacggtgcgt atcaggcact tcctgttgag agcaccgctc acgagcttgc atttgatcac
      661 gaaggtcgca cctggctttt gcggcaaaga ccagcgggca ctccaaactc tctgattgtc
      721 atggaccagt ccgccggcat catcgactcg aactcctttg ttgaagatga tttcccgatt
      781 ggaaccccac acaccaacct ggcgtatgac gttgtcacca agaagattgt cgttggctta
      841 aggtctgcga gtaatcagct tcagctgtat acgttcgaca cttcggccaa gttctagcac
      901 acctgaaacc ccatgcgacc ccggcacact gccggggttg tctttttctg catggtgaca
      961 acattccgtt cgactgtgat acaatagtga gaaaccgtct acagggaagg aatagagtat
     1021 ggagaagact ttccggttcg ggttgattct cgttgttgcg ctgatgatct gccacacgag
     1081 ctttggggcg gcagcagctg agcgttggtt cgggatgatc ccgacggcga agatcaagga
     1141 agtgcgctcg acgctgagcg gagcgaacgt ttcgtatacc acctttggca cgctgacggc
     1201 cgcgatcggc ggcgagaggc tactcacgtg gttgaaaaag catcctggca gcggggaacc
     1261 ggtacagttg gccgccagcg aaaagctgtg gttgatgacc accaaagacc ccgaactgca
     1321 gacggtcact tttcccgggg ttcgccaact ctggaagggc t
//
LOCUS       DAORHD010001475         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_136686_length_1359_cov_4.386503, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001475 DAORHD010000000
VERSION     DAORHD010001475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136686_length_1359_cov_4.386503"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="PKO06_22750"
     CDS             <1..1078
                     /locus_tag="PKO06_22750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72547.1"
                     /translation="LNSILGFADLLLGSFQGELNPKQRAYVGTIASAGKHLLDLINDL
                     LDMARIEAGIIKLHREPLSLREIIDSLVQFVQPSTQEKQLMVSITVGNNADQAFADPA
                     RTKQILLNLLSNAIKFTPPQGTITIETREHGGNQVMVTVSDTGIGIESEDQRKVFTTF
                     FQSRVAQKGQFGGTGIGLALCKRLVELQAGTIGFESHPGHGSHFWFTLARVAPPVAQG
                     SETETEHRIGYFPSGRVLIVASDDMVVESIQDLVHLSGCRVDVARDLRQMIDFAIGQP
                     PDLIVLDLRLAGSGPESAPTQLRSQALTARIPLIGIGRSTDAPPRLPLPDDDFSATFN
                     LPIPIAPFSDLLRRLLDNKERKPE"
     gene            1075..>1359
                     /locus_tag="PKO06_22755"
     CDS             1075..>1359
                     /locus_tag="PKO06_22755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72548.1"
                     /translation="MIGTTPLNSCPLPGRARILITDDLEPNRLLLAELCQSLGHLTSE
                     AEDGFVCLEQARANHPDLILLDLLMPRLNGFETLAQLKADPDLLRIPVIII"
BASE COUNT          292 a          433 c          341 g          293 t
ORIGIN      
        1 actcaacagc attctcggtt tcgccgatct gttgctcggt tcatttcaag gcgaactgaa
       61 tcccaaacaa cgcgcctatg tcggaactat cgcaagtgcc ggcaagcatc tgctcgatct
      121 gatcaacgac ctgctcgaca tggcccgcat tgaggccggc atcatcaagc tgcatcgaga
      181 acctctttcg ctgcgtgaaa tcatcgacag tctcgttcaa ttcgtacaac catcaaccca
      241 ggaaaaacag cttatggtca gcattaccgt tggaaacaat gccgatcagg cgtttgccga
      301 cccggccaga accaaacaga ttctgctgaa cctgttaagc aacgcgatca aattcacccc
      361 accgcagggg accatcacca tcgaaacccg cgagcacggg ggcaatcagg tcatggtcac
      421 ggtcagtgat accggcatcg gaatcgaatc cgaagaccaa cgcaaagtct ttaccacgtt
      481 tttccagagt cgtgttgcgc agaaaggcca gtttggcgga acgggaatcg gtctcgcgct
      541 ctgcaagaga ctggtcgaat tgcaggctgg gacgatcggt tttgaaagtc accctgggca
      601 cggcagtcac ttctggttca ctctggctcg cgtggctccc cccgtcgctc agggttccga
      661 aaccgaaacg gaacatcgta tcggctactt tcccagtggt cgcgtattga tcgtggcgtc
      721 tgacgatatg gttgtcgagt cgattcagga tctcgtacat ctcagcggat gtcgcgtcga
      781 tgtcgcccgt gatctgcgcc agatgattga tttcgccatc gggcaaccgc cggacctgat
      841 cgtcctcgat cttcggctgg ccggttccgg ccccgagagt gccccgacac aactgcgttc
      901 acaagccctg acagcgcgaa ttccgctcat cgggatcgga cgctcaaccg atgccccccc
      961 gcgccttcca cttccagatg acgatttttc ggcgaccttc aatctgccga ttccgatcgc
     1021 gccgttctcg gatctgctgc gaagactgct ggacaacaaa gaaaggaaac cggaatgatc
     1081 ggcaccacgc ccctcaactc ctgcccactc ccgggtcgag ctcgcattct gatcaccgat
     1141 gacctcgaac cgaaccggct tcttctggcc gagctgtgtc agtctctcgg ccacctgacc
     1201 tctgaagcgg aagacggttt cgtctgcctg gagcaagctc gcgcgaacca ccccgacctc
     1261 attcttctcg atcttctcat gccccgcctg aacggcttcg agactctggc ccagctcaaa
     1321 gccgatccgg atctcctgcg tattccggtg atcatcatc
//
LOCUS       DAORHD010001476         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_136693_length_1359_cov_3.903374, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001476 DAORHD010000000
VERSION     DAORHD010001476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136693_length_1359_cov_3.903374"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1359
                     /locus_tag="PKO06_22760"
     CDS             29..>1359
                     /locus_tag="PKO06_22760"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdrA family protein"
                     /protein_id="HNV72549.1"
                     /translation="MAIRFVIRKDAYFDSVTLMLINSEVKKLPQVESAVVGMATDYNL
                     DSLKRLGLMQPEMTKATPSDLVLCVKAATEKVAEEALVTAEKLLTARKKGGKTQDLRP
                     STLEKAFQQNPDSNIVLISVPGAFAARETETALRNNRHVMLFSDNVSVADEIRLKELA
                     CKKGLLVMGPDCGTAIINGVPLAFANVVRSGPIGLVAASGTGLQEVTSLIHSLGSGIT
                     QAIGVGGRDLSEKVGGLMTLQAVKALAHDDATKVLVLISKPPAESTMVKLFKELKNVK
                     KPVVVYFIGADRRLIENEGHTAAANLEEAAIQACRLAGVAQAAPLMSDKEIAKLAKRD
                     KIPGKYLRALYSGGTLCDESQRLLHAQLPNMHSNTPVKGVKEMKDPYQSEEHVIIDLG
                     DDQFTRGKAHPMIDPSYRQERIVQEMKDRDVALIAFDVVLGYASLTSERLG"
BASE COUNT          332 a          379 c          398 g          250 t
ORIGIN      
        1 ccgacagaaa gaaaggaata acaatcgcat ggccattcgt ttcgtgattc gcaaagacgc
       61 gtatttcgat tcggtcaccc tgatgctcat caatagcgag gtcaagaaac tcccccaggt
      121 agaaagtgcc gtggtgggca tggcgaccga ctacaacctg gacagtctca aacgtctcgg
      181 gttgatgcag ccggaaatga cgaaagccac gcccagcgat ctggttctct gcgtcaaggc
      241 agcgactgag aaggtcgccg aggaagcgct ggtgacggcg gaaaagctct tgaccgctcg
      301 caagaagggc ggcaaaactc aggacttacg cccgtcaaca ctcgagaagg cttttcaaca
      361 gaacccagac agcaacattg tgctgatctc ggttcccgga gcattcgcgg cacgcgagac
      421 cgaaacggct ctgcgaaaca accgacatgt catgctgttt tccgacaacg tgagcgttgc
      481 cgacgagatt cgtctcaagg aactggcctg caagaaaggg ttactggtca tggggcccga
      541 ctgcggcaca gccatcatca acggcgttcc cctggcattc gccaacgtgg tacgttcggg
      601 tcccatcggc ctggttgctg cttcaggcac tggtctgcag gaagtgacca gcttgattca
      661 ttcgctgggc tccggcatta cgcaggcgat cggggttggc ggtcgcgatc tcagcgaaaa
      721 ggtcggcggt ctgatgaccc tgcaggcagt aaaggctctg gcgcacgatg acgcaacgaa
      781 agtgctggta ctgatttcga agccacccgc cgaatcgacg atggtgaaac tgttcaagga
      841 actgaagaac gtcaagaaac ccgtggtagt ctatttcatc ggcgccgacc gacggctcat
      901 cgagaacgaa ggacatacag ccgccgcgaa ccttgaggag gcggcgatcc aggcctgtcg
      961 gctcgccgga gtggctcaag cggctccgtt gatgagcgac aaggaaatcg ccaaactggc
     1021 caagcgagac aagatacccg gcaagtatct tcgcgcgctc tactcaggcg gcaccctctg
     1081 cgatgaatcg cagcgtctgc tgcacgcaca gctgccgaat atgcattcca acaccccggt
     1141 caaaggggtc aaagaaatga aagaccccta tcagagcgaa gaacacgtga tcatcgatct
     1201 gggtgatgat cagttcaccc gtggcaaagc gcatccgatg atcgacccgt cgtatcgcca
     1261 ggaacgcatt gtccaggaga tgaaagatcg agatgtggca ttgatcgctt tcgacgtggt
     1321 gctcgggtac gcgtccctca cgtccgagcg gctcgggga
//
LOCUS       DAORHD010001477         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_137321_length_1356_cov_2.504228, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001477 DAORHD010000000
VERSION     DAORHD010001477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137321_length_1356_cov_2.504228"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PKO06_22765"
     CDS             complement(<1..1040)
                     /locus_tag="PKO06_22765"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72550.1"
                     /translation="MLEEFGRDLSALAAVGALEPLIGRAEEVRRMIDILSMQGKNNPI
                     IVGPPGVGKTCIVEGFAQVLNVGSVPEPLKGKKIFQLDFTAIVSGTIYRGQLEERIQR
                     LIDELLEHREYIVFIDEFHTIALPTLNRSFDFANVLKPYLAKGEITIIGATTEEEFKK
                     YVEEGDPALARRFVKVRVGEPPKNELKQILQRTSKRMGSRYGMVIPEVTIDRLIDLSD
                     QYIKDRYFPDKAIDLLKEALAEKRFNMSADGMRGDLQVLENILDTEIKAVETGDLPGV
                     EKALAAWDEKKNMIFLRTLTPDDIATMLAKRTGAVIGDNQLVETHKRIQDLDKAFGVS
                     IIGQKRAKDAVLG"
     gene            complement(1147..>1356)
                     /locus_tag="PKO06_22770"
     CDS             complement(1147..>1356)
                     /locus_tag="PKO06_22770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72551.1"
                     /translation="DISQRMQNLPSVPSKYAVLKMTLPPTITCGTVHTLAELCQRISG
                     ADCGVSLGVRFHRNPSLEVAFTQFI"
BASE COUNT          275 a          375 c          385 g          321 t
ORIGIN      
        1 ccgagaaccg cgtctttggc ccgtttttga ccaataatcg aaacgccaaa ggctttgtcc
       61 agatcctgta tgcgcttgtg ggtctcgacc aactggttgt cgccgatgac ggcgccggta
      121 cgtttggcca gcatcgtggc gatatcatcg ggggtgagcg tgcgcagaaa gatcatgttc
      181 tttttttcgt cccaggcggc aagtgctttc tcgaccccgg gcagatcgcc ggtctcgacg
      241 gccttgatct cggtgtcgag gatgttttcc agcacctgca gatcaccgcg catgccgtcg
      301 gccgacatgt tgaagcgttt ctccgccagc gcttctttca acaggtcgat cgccttatct
      361 gggaagtaac gatctttgat gtactggtca gaaaggtcga tcagtcgatc aatggtcact
      421 tcgggaatga ccatgccgta gcgactgccc atgcgtttgc tggtgcgctg aagaatctgc
      481 ttcagctcat tcttgggcgg ttctccaacc cggactttca cgaaacgccg agcgagcgcg
      541 gggtcgcctt cttccacgta tttcttgaat tcttcttccg tggtcgcgcc gatgatcgtg
      601 atttcaccct tggccagata gggcttcagc acgttggcga aatcgaacga acgattcagc
      661 gtcggcaggg cgatcgtgtg aaactcgtcg atgaacacga tgtattctcg atgttcgagc
      721 agttcgtcga tgagacgctg aatgcgctct tcgagctgcc cgcggtaaat cgtgccgctg
      781 acgatggcgg taaagtcgag ctgaaagatc ttcttcccct tcagcggttc aggaacactg
      841 ccgacattca gcacctgcgc aaacccttca acgatgcagg tcttgccgac ccccggcggc
      901 cccacgatga tcgggttgtt cttgccctgc atcgacagaa tgtcgatcat gcgtcgaact
      961 tcctcggcac ggccgatcag gggttcaagg gcgccaacag cagccagggc gctgagatct
     1021 cgcccaaatt cctcgagcat ggtggaactc ctctttggta tgtagtttgc gccatcatac
     1081 cggaatccct ttctctatac aaactgtatc agccggggaa aatgcctgct gtaagacaca
     1141 accgaatcag atgaattgag tgaatgccac ttcgagagaa gggttccggt gaaagcgaac
     1201 gccgagagac acgccgcaat cggctccgct gatccgctgg cacagttccg cgagcgtgtg
     1261 aacggtgccg caggtgatgg tcggtggcag ggtcatcttg aggacggcat attttgacgg
     1321 caccgatggc agattctgca tgcgctgaga gatgtc
//
LOCUS       DAORHD010001478         1354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_137690_length_1354_cov_2.387991, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001478 DAORHD010000000
VERSION     DAORHD010001478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137690_length_1354_cov_2.387991"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1354)
                     /locus_tag="PKO06_22775"
     CDS             complement(<1..>1354)
                     /locus_tag="PKO06_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72552.1"
                     /translation="PLPEAQTLAPYADYPGSEVEPGADERRQYQKHHYIWDFHPSGAR
                     AVAVVHVAEKPTLLRGDGVSGETVYCLREYGPQYKIYNEDVVGAWVAAAGGAEPVSVS
                     ERAVLEVEFHITITGEKDTDFEFAVTEKRTLSNYWYFDAQYAYCDSRLEALGVLAGDL
                     LTDEIRLFGDVEFGNTTPTVFDEFVVTRNQDRGFADVQSVCVAQNRPYRMLDEFRWIE
                     RDDLPTYDGDPPAIYRYYISEYPTPGANALARMVSSDLRSLSKVFHQSRNGDPFGLHV
                     IAFGETRHGSAIPHTTTDSLALGHGLLPAKFLPSGSPWLNGRAVNPDSLEAAHCIGLH
                     RVAWDHITYRYGYDASVHSGICTHPDGHFAVFAFFKNPYNPDAPVEPFDLIEYRRVEL
                     DPDGNPVETFERTTHVEAFSKVFAREFDLPAFVAAITTDKLMVIQRFASWRNIKLPKL
                     A"
BASE COUNT          289 a          403 c          398 g          264 t
ORIGIN      
        1 gccagcttcg gcagcttgat gtttcgccaa ctggcgaacc gctgaataac catcagctta
       61 tcagtagtga tcgccgcaac aaacgcgggt aggtcgaact cgcgcgcaaa caccttggag
      121 aacgcctcaa cgtgtgtcgt gcgctcgaac gtctcgaccg ggttcccatc ggggtccaac
      181 tcgacccgcc tgtattcaat gagatcgaac ggctcgaccg gggcatccgg gttgtacggg
      241 ttcttgaaaa aggcaaacac cgcgaagtgc ccgtctgggt gggtgcagat gccagagtgc
      301 accgatgcgt cataaccata ccgataggtg atgtggtccc acgcgacacg gtgcagcccg
      361 atgcagtgtg cggcctctag actgtcagga ttgacggccc tcccgtttag ccagggtgag
      421 cccgacggga ggaatttggc cggcagcagc ccatggccaa gcgccaatga gtctgtcgtg
      481 gtatggggta ttgccgaacc gtgtcgagtc tcgccaaagg cgataacgtg caagccgaac
      541 gggtcaccgt ttcgactttg gtgaaacacc ttcgacagtg agcgaagatc agacgacacc
      601 atgcgagcga gcgcgttagc ccctggcgtg gggtattcag aaatgtaata tctgtagatg
      661 gccggcgggt cgccgtcata ggtcggaaga tcgtcacgct cgatccaccg gaactcatcg
      721 agcatccggt acggtcggtt ttgcgcaaca cagacgctct gcacgtctgc gaaaccgcgg
      781 tcttggttcc tcgtgacgac aaactcgtca aacaccgtcg gggtcgtgtt tccgaactcg
      841 acatcaccaa acagacgaat ctcgtcagtc agaaggtcac ccgccagcac accgagggcc
      901 tcaagcctgc tgtcgcaata tgcgtattgc gcatcgaaat accagtagtt gctgagcgtg
      961 cgtttctcgg tgacggcgaa ctcaaagtca gtatctttct cacccgtgat cgtgatgtga
     1021 aactcgacct caaggacagc gcgttcactg acgctgacag gttccgcccc gccggccgca
     1081 gcaacccatg cacccacgac gtcctcgttg tagattttgt attgcggccc gtactcgcgc
     1141 aggcaataga ccgtctcgcc actcacaccg tcgccgcgta agagcgtcgg cttctcggca
     1201 acgtggacaa cggccacggc ccgagcacct gacgggtgga agtcccagat gtagtgatgc
     1261 ttctggtact gacggcgctc gtcggccccc ggctcaacct cagaccctgg gtagtcagca
     1321 taaggggcga gcgtctgcgc ctctggaaga gggg
//
LOCUS       DAORHD010001479         1353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_137789_length_1353_cov_3.626348, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001479 DAORHD010000000
VERSION     DAORHD010001479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137789_length_1353_cov_3.626348"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..>1353
                     /locus_tag="PKO06_22780"
     CDS             126..>1353
                     /locus_tag="PKO06_22780"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV72553.1"
                     /translation="MKIKSQIHLLIFVVLIISMMIPMGTTLYLLYRQASSESSRFRTA
                     EIEQARNELCDLVDLVWADIQDSYQDVTRRENLETRVGKTLKVMVSFLEQVLNSKMAQ
                     VKKGERTLENAREYTLGELRSIRLANGGYFWLQDTTQPFPRMVMHPLRPDLEGQILSS
                     TTFNVARHKGDNLFVAAAEEANKPEGGFVDYLWEKSLPDGTQLKNLPKFSYVRALPDW
                     NWVIGIGIFLDEIIEQAAEDIKSELRHLRYNNGNGSFWVTDLKGTVLVHSDAQLIGRN
                     LGQDNALPYVRLAMAQIADHKPAGALEFETTSQTQNANSSFISYYRVFPPLGWIIGSN
                     RNVDFIARNAAQQERQVWQNMWVLIGLMSILFITLLGIPLRVTSHVLGELGLVDPETP
                     ETSITITRRCTSTWVAA"
BASE COUNT          305 a          410 c          351 g          287 t
ORIGIN      
        1 ctctcatcca gtatcgcgat aacctgatcc ccaaagtgct tttctggccc ggtgtcctgg
       61 ggattctcct gctggtcacg atcagccatt tttacccgta acgagattcc acacgaggtt
      121 ccactatgaa aatcaaatct cagatccacc tgctgatctt cgttgttctg atcatctcga
      181 tgatgattcc catgggcacg acgttgtacc tgctctatcg acaggcaagc agcgaatcaa
      241 gccgatttcg gaccgctgaa atcgaacagg cacggaacga gttatgcgat ctcgtcgacc
      301 tcgtgtgggc agacatccag gacagctatc aggatgtcac acgtcgcgag aacctcgaaa
      361 cacgcgtcgg caagacgctc aaagtcatgg tttcctttct cgaacaggtc ttgaacagta
      421 agatggccca agtcaagaaa ggggaacgca cgctcgaaaa cgcccgcgaa tacaccctcg
      481 gcgaactgcg cagcatacgt ctcgccaacg gtggctactt ctggctgcag gatacgacgc
      541 agccgttccc tcggatggtg atgcaccccc tgcgtcccga tctcgaaggg cagattcttt
      601 cgtcgaccac gttcaacgtc gcacgccata aaggcgacaa tctcttcgtt gccgccgccg
      661 aagaagcaaa caaacccgag ggggggtttg tcgactatct ctgggagaaa tccctgcccg
      721 acggcacgca gctgaagaac ctgccgaaat tttcgtacgt tcgtgccctg cccgactgga
      781 actgggtcat cgggatcggc atctttctcg acgagatcat cgaacaggcc gccgaagaca
      841 tcaagtccga actgcgccac cttcgctata acaacgggaa cggctcgttc tgggtcactg
      901 atctgaaagg caccgttctt gtgcactctg atgcgcagtt gatcgggcgc aatctcggcc
      961 aggataacgc tcttccgtat gtccgactcg ccatggccca gatcgcagac cacaagccag
     1021 ccggcgctct cgagtttgag accacgagcc agacccagaa tgccaattcc agctttatca
     1081 gctactatcg cgtctttccg ccgctcggct ggatcatcgg ttccaatcgg aatgtcgact
     1141 tcatcgccag gaacgcggca caacaggaaa ggcaggtctg gcagaacatg tgggtcctca
     1201 tcggcctgat gagcattctg ttcattaccc ttctcgggat tcctttacgg gtcacgagtc
     1261 atgtactcgg tgagctcggg ctggtggacc ccgaaacgcc tgagacgtcg atcacgatca
     1321 cccggcggtg cacgtccacc tgggtggcgg cca
//
LOCUS       DAORHD010001480         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138024_length_1352_cov_2.559753, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001480 DAORHD010000000
VERSION     DAORHD010001480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138024_length_1352_cov_2.559753"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKO06_22785"
     CDS             complement(<1..921)
                     /locus_tag="PKO06_22785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNV72554.1"
                     /translation="MNHPRGYVVLILALVLVLSGVTPAIATDRRMDLTTSTDAVPSAG
                     GGRVALLIGNSDYEHAALPNPVNDVRAFATTLKDIGWDVIQRENCDILAMEEAAKEFN
                     DRLRKCDIGLFYYSGHGCQIDGENFLVPLKANLSKPLEIRHKCFPAKLLVDYMEDAGS
                     KLNIIILDACRDNPFKGSSRSLSSGLAKMDLAPGRQMLLAYATDPGNVAQDGDPGSNS
                     PFMKHMLKNIRTPGLDINKLFREVRKGVRSETGEKQRPWETSCLMDDFFFVPPDAVAP
                     AVGPQATTGLATGTVPPAASDSTTAVSAAAP"
     gene            complement(1046..>1352)
                     /locus_tag="PKO06_22790"
     CDS             complement(1046..>1352)
                     /locus_tag="PKO06_22790"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72555.1"
                     /translation="DDFNRGRQAAGMFTIKTGIGIGSGEVLMGTLGGSGRQDFTVTGI
                     TVNRAAAMEKRSKKARSSFIVVCAETAKRLGAGQSLTQIAADVDTDPAAIEGYELIL"
BASE COUNT          295 a          382 c          392 g          283 t
ORIGIN      
        1 cggcgccgcg gcggaaacgg cggtcgtcga atcggatgcc gcaggaggaa ccgttccggt
       61 cgccaggccg gtcgtggctt gtggaccaac ggccggagcg acagcatcgg ggggaacgaa
      121 gaagaagtcg tccatgaggc acgacgtttc ccaggggcgc tgtttctcac cagtttcgga
      181 gcgaactccc ttgcgcactt cacgaaacag tttgttgata tcgagacccg gcgtgcgaat
      241 gtttttgagc atgtgcttca tgaagggcga gttgctgcct ggatcgccat cctgcgccac
      301 attaccgggg tcggtggcat aggcgagcag catctgtcga ccaggagcga gatccatctt
      361 ggccagaccc gagctgagac tgcgtgaact acccttgaac ggattgtcgc gacaggcatc
      421 gagaatgatg atgttcagct tggaacccgc atcttccatg taatcaacca gcaacttggc
      481 cgggaagcat ttatggcgga tctcgagcgg cttgctcaaa ttcgccttga ggggaaccag
      541 gaagttttcg ccatcgatct gacacccgtg gccggaatag tagaacaggc caatatcgca
      601 tttgcgcagt cgatcgttga attctttcgc ggcttcttcc atggcaagaa tgtcgcagtt
      661 ctcgcgttga atgacatccc acccgatgtc tttcagcgtg gtcgcgaacg cccgcacgtc
      721 gttgaccggg ttgggcaacg cggcatgttc gtagtcgctg ttgccgatca gaagagcaac
      781 gcgcccgccc ccggcagaag gaacggcgtc agtcgaggtc gtcagatcca ttcgacggtc
      841 ggtggcgata gccggggtca cacctgacag aacaagaacc agggcaagga tcagaacaac
      901 gtacccgcgc gggtgattca tatcagactc ccgaagattc tctgaattcg aaacttcaaa
      961 taggcaaatg gctcgcgaag acagtgtacc gcatctacaa ccatacacgc aatggggtgc
     1021 cgacgagacc aggtctggtt ctcgctcaca ggatcagttc atagccttcg attgccgcag
     1081 gatctgtatc gacatcagcg gcgatctgcg tgagagactg accggcaccg agtcgttttg
     1141 ccgtctctgc acagacgaca atgaacgatg agcgtgcctt tttcgaccgc ttttccatcg
     1201 ccgccgctcg gttcacggtg atgcccgtca cggtgaagtc ctgtcgaccg ctgccgccga
     1261 gagtacccat caagacttca ccagatccta ttcctatgcc ggttttgatg gtaaacattc
     1321 cggcagcctg gcgaccacgg ttgaaatcgt cc
//
LOCUS       DAORHD010001481         1351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138158_length_1351_cov_2.913580, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001481 DAORHD010000000
VERSION     DAORHD010001481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138158_length_1351_cov_2.913580"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="PKO06_22795"
     CDS             complement(<1..994)
                     /locus_tag="PKO06_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72556.1"
                     /translation="MRRTRAMTLTEVLVALFIFIGFAGGVYYLIKEVNVKRAIGMARS
                     MATNEAGKVLSVMQQDFAAAKLGSFKNLDEIKPSFKVGVGKVGQEMTVTYEYKKPTLT
                     RTIGPWQWTIAKNLDEFKVSKQLATGQVLIEVKSKVAIDGLAENRAQIHEQNQMVIMR
                     ADNSAMNDPHWRDVGDATGLVSMKGDLIAGIETDMEDFVGNAIDELGELGGTVNVGAL
                     AGASTAELAQKLSSQWQQILGQLDSIDQRIQNTAPEGILKFPEDVWEKIYYNEDNMKS
                     CANAMKTCLVNQKTLSSMSMDPIRNIASGYGYNNRVRETVQGLFDAKKSVFQAGQ"
     gene            complement(1011..>1351)
                     /locus_tag="PKO06_22800"
     CDS             complement(1011..>1351)
                     /locus_tag="PKO06_22800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72557.1"
                     /translation="QSVKEEMLGTPFKTLVSMTSQLQKDTDGKYQLVDGFYQSTNQKV
                     LEIQKKYKDFQIEGKIWYVDRRTDPNAAEVLDCIQVDAKVLWHYPGNESSDGVAKAER
                     TRSFMIVAPK"
BASE COUNT          286 a          413 c          305 g          347 t
ORIGIN      
        1 gctggccggc ctgaaacacg cttttcttcg cgtcgaacaa gccctgcacc gtctcgcgga
       61 ctcgattatt gtacccgtag ccgctggcga tattgcgtat gggatccatg ctcatcgaac
      121 tcagagtttt ctgattcacc aggcaggtct tcatcgcgtt ggcacagctc ttcatgttgt
      181 cttcgttgta ataaatcttt tcccagacat cttcggggaa cttgagaata ccctcgggag
      241 cagtattctg aattcgctga tcaatggaat cgagttgtcc gagaatctgc tgccattgtg
      301 acgacagctt ctgggccagt tctgccgtac tggctcccgc caacgcaccc acgttcacgg
      361 tcccgcccag ttcacccaac tcgtcaatcg cattcccgac aaagtcttcc atgtcggttt
      421 caatgccggc aatcaggtcc cctttcatcg agacgagacc agtggcatca ccgacatcgc
      481 gccaatgcgg gtcattcatg gccgaattgt ctgcacgcat gatgaccatc tgattctgct
      541 cgtgaatctg cgcccggttc tcggcaagcc catcaatcgc aaccttcgac ttcacctcga
      601 tcagcacctg gcccgtggcg agctgcttcg aaaccttgaa ttcatcaaga tttttcgcaa
      661 tcgtccactg ccagggaccg atggtgcggg tcaacgtagg tttcttgtac tcgtaggtaa
      721 ccgtcatttc ctgaccgacc ttgcccacgc ccaccttgaa cgatggcttg atctcatcaa
      781 ggttcttgaa tgatccaagc ttggcggcgg cgaaatcctg ctgcatgacc gaaagcacct
      841 tgccggcctc attggtcgcc atggaacgcg ccatgccaat agcccgtttg acgttgactt
      901 ccttgatgag ataatacacc ccaccggcaa aaccaatgaa aatgaacagg gccaccagaa
      961 cctctgtcag ggtcatcgct cgtgttcgcc gcatatcgcc ctcctttgcc ctacttgggg
     1021 gcaacgatca tgaatgaacg ggttcgttcc gccttggcta caccatccga actttcattt
     1081 ccaggataat gccacagcac tttcgcatcc acctgaatgc aatcaagcac ttcggcggca
     1141 ttcggatctg ttcgccgatc cacataccag atcttgcctt cgatctgaaa gtccttgtac
     1201 tttttctgga tctcgagcac cttctggttc gtcgattgat agaacccgtc aaccaactgg
     1261 tactttccat ccgtgtcctt ctgcaactga gaggtcatcg aaaccaacgt tttgaagggc
     1321 gtccccagca tttcttcctt gacgctctgc a
//
LOCUS       DAORHD010001482         1348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138616_length_1348_cov_3.943542, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001482 DAORHD010000000
VERSION     DAORHD010001482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138616_length_1348_cov_3.943542"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="metK"
                     /locus_tag="PKO06_22805"
     CDS             complement(<1..855)
                     /gene="metK"
                     /locus_tag="PKO06_22805"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNV72558.1"
                     /translation="MSKSSHYFFTSESVTEGHPDKVADQISDAVLDAIIGQDPKARVA
                     CETAVTTGLAVVMGEITTSCYVDVNSIARKTLCEIGYDNAVYGIDGKSCGVMVALDEQ
                     SPDIAQGVNKAQDGSDKDAELNIGAGDQGMMVGFAVRETPELMPAPIFYAHQLCHKLA
                     EARRAKTIGYLRPDGKSQVTIEYDNGKPVRVDAVVVSTQHHPEASHAQITEDVIEKII
                     RPVIPAELLDKKTKFYVNPTGRFVVGGPQGDTGLTGRKIIVDTYGGMAAHGGGAFSGK
                     DPTKVDRSG"
BASE COUNT          267 a          371 c          397 g          313 t
ORIGIN      
        1 gccggagcgg tcaaccttgg tgggatcttt gccggagaac gcgccaccac cgtgcgcggc
       61 catgccgccg taggtgtcga cgataatttt gcgaccggtc aggccggtgt cgccctgggg
      121 gccgcccaca acgaaacgac cggtcggatt cacgtagaac ttggtcttct tgtcgagcag
      181 ttccgcggga atgacggggc gaatgatctt ctcgatcacg tcttcggtga tctgggcatg
      241 actggcttcg ggatggtgct gggtcgacac gacgactgca tcaacgcgaa ccggtttgcc
      301 gttatcatac tcgatcgtga cctggctttt tccgtctggg cgcagatagc cgatggtttt
      361 tgcccggcgg gcttcagcca gtttgtgaca cagttggtgg gcatagaaaa tcggtgcggg
      421 catcagctcg ggggtttcgc gaaccgcgaa cccgaccatc atgccctgat ctccggcacc
      481 gatgttcagt tccgcgtctt tgtccgatcc gtcctgggct ttattgacgc cctgagcgat
      541 gtctggagac tgttcatcga gagcgaccat aacgccgcaa ctctttccat cgattccgta
      601 aacagcattg tcatacccga tttcgcacag tgttttgcgt gcaatactgt tgacatcaac
      661 atagcaggag gtcgtgatct cgcccatgac aaccgccaga ccggtggtga cagcggtttc
      721 gcaggcgacg cgggctttgg gatcctggcc gatgatggcg tcgaggacgg catcggaaat
      781 ctggtcggca accttgtcag gatgcccttc ggtaaccgat tccgaagtga agaaataatg
      841 agaactcttg ctcatgacaa atcctccgcg gataaggtgc ggggattata acagtggccc
      901 gataccccgt caagacgggg attttgtcgg gtacacgaaa gattgggaag ggagtacccc
      961 cggccaagcc gggggtacga tggtcacctc gtctggctgc ctgcaatttg cggtctcgct
     1021 tcggtttggt tcaacattgt cgtaatcttc ttgtacgcga tctcgtagct gcagacgaac
     1081 ccgataactc ccgagagaat gaccaagatg gtggcgatca ccctcatggt agaccacctc
     1141 cttccgtgtg accgaagtat cggcaggagt gtttctttcg gtaatgcgac ggcagaaaaa
     1201 cagaacgttg acccttccct gccgagatga ctataatcgt tgccgatgaa gaatttttgg
     1261 aactgcctgc ttctgacgag ctttctcgtt gccttgctgt gcccggcgcc tcgtctcgcc
     1321 ggccagtcgc cgaccgacgg gaacgggc
//
LOCUS       DAORHD010001483         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138778_length_1347_cov_4.630031, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001483 DAORHD010000000
VERSION     DAORHD010001483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138778_length_1347_cov_4.630031"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKO06_22810"
     CDS             complement(<1..256)
                     /locus_tag="PKO06_22810"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV72559.1"
                     /translation="MQPRCAYPWQQMVIDLSGEVSPCCFWRGYGGDALPLGQARQASL
                     VDIWNGEPWRRLRHDLSHGRLTGYPCATCLSTQWNGGDPPP"
     gene            complement(276..>1347)
                     /locus_tag="PKO06_22815"
     CDS             complement(276..>1347)
                     /locus_tag="PKO06_22815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72560.1"
                     /translation="AASVAETPVAATPVKVVTPPPASVTSMAPAATLMTPPPRDTRQF
                     RALKLESVELAAGLPPVVPVVAAPGAELNRALGGGFAQRGLHLLVGTREEGKAAWLLS
                     LALQSAVRVPVLYLSREHTMAELTLRATSWVSRQAASESIMRSVGTGAEAQAARQKWQ
                     QTFETTCGVLPDSLFVRGSDRSVRLTVPDDIVELLKLLPPGPAGKVVFLESMNLDDLT
                     DEYLAQLRHRAVSHQFTVFLSAHLGSTAWPRPHLIEGPDLDLLARFQGIVDTLTHVTS
                     DRLNLRRFIAMANGKVEPPMVEKLEHQLQQASSGPRLRHDTYALVRLLHARGGSRRLI
                     LSLYQREIGRFWEGPSLPLSRP"
BASE COUNT          274 a          421 c          423 g          229 t
ORIGIN      
        1 acggtggcgg gtctccgccg ttccattgcg tcgacaggca ggtggcgcag gggtacccgg
       61 tgagtcgccc gtgcgacaga tcatggcgca gacggcgcca gggctcaccg ttccagatat
      121 cgacgagaga agcctgccgc gcttgtccaa gcgggagcgc gtctccacca tagccgcgcc
      181 agaaacagca tgggctgacc tctccagaga ggtcgatcac catctgctgc cagggataag
      241 cacatctggg ttgcatgatg gtgtcgtcga tcctgtcagg gccgcgacag cggcagtgac
      301 ggaccttccc agaagcgacc gatctcgcgt tgatagagcg agagaatcag gcgacgcgag
      361 ccacccctgg catgcagcag gcgtaccaag gcataggtgt cgtgacgaag gcgtgggcca
      421 ctgctcgcct gctgtaactg gtgctccagt ttttccacca tcggtggttc taccttgccg
      481 ttcgccatcg caatgaatcg tcggagattg agccgatctg aggtcacatg cgtcagcgta
      541 tcgacgattc cttgaaagcg agccaacaga tcgaggtctg gaccttcgat caggtgcggc
      601 cgcggccacg cagtcgaccc gagatgcgcc gacagaaaga ccgtgaactg atgcgagaca
      661 gcccgatgac gcaactgtgc gagatactcg tccgtgagat cgtccaggtt catcgactcg
      721 agaaagacca ctttaccagc cggaccgggt ggcagaagtt tcagaagctc gacgatgtca
      781 tccggaaccg tcagacgtac agaccgatcg ctgccacgca cgaaaaggga atcaggtaac
      841 acgccgcagg tcgtctcgaa cgtttgctgc cacttctgac gggcggcctg cgcttccgcg
      901 ccggttccca cgctgcgcat gatgctttcc gacgcggcct ggcgactgac ccatgaggtg
      961 gctcgcaacg tgagttccgc catcgtatgt tcgcgcgaca gatagagtac aggaacgcgc
     1021 acggcactct gcagagccag agacaataac caggcagctt tcccctcttc acgggtgccg
     1081 accagcagat gcaacccgcg ctgcgcaaag ccgccgccca aggcccggtt cagttctgct
     1141 cccggagcgg caaccacggg cacgaccggt ggcaggcccg ccgcaagttc cacggactcc
     1201 agtttcagcg cgcgaaactg gcgcgtatcg cgtggcgggg gagtcatcag cgtcgccgcg
     1261 ggtgccatcg aagtgacact ggctggaggt ggcgtcacaa ccttcaccgg agtggccgcg
     1321 acaggggttt ccgccacgct ggccgcg
//
LOCUS       DAORHD010001484         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138819_length_1347_cov_3.010062, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001484 DAORHD010000000
VERSION     DAORHD010001484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138819_length_1347_cov_3.010062"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1132
                     /locus_tag="PKO06_22820"
     CDS             35..1132
                     /locus_tag="PKO06_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV72561.1"
                     /translation="MSRKTEILVAGGGPAGMSAAIVAAQLGAKVLLVDDNRVLGGQLV
                     KQTHRFFGSSARDAGIRGIAIADELQKQVAAQKKQLTVLTETAVVGFYSDNQAGILSH
                     KAYEPVTYERAIVCAGATENSLSFENNDLPGIYGAGAVQTLMNVHGIRPGKKVLMIGS
                     GNIGLIVSYQLLQAGVDVVGLVEALPTIGGYLVHASKIRRLGVPIYTCHTIKQAIGTN
                     HVEGAALVRIDERFQPVPNTEFQVECDCICLAVGLSPLIDLVQQGGAKMVFVPELGGL
                     VPLHDGDMRTTSPGVYVAGDASGIEEATTAILGGRIAGARAYESLHGKKPEAEAIVVD
                     ANNELKTLRSGSTGERIRWGRDRLLKEIAAC"
     gene            1126..>1347
                     /locus_tag="PKO06_22825"
     CDS             1126..>1347
                     /locus_tag="PKO06_22825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HNV72562.1"
                     /translation="MLKNDGIPTREEYETILPTPERMKKGPVAIIECFQDIPCDPCVT
                     ACPTKAIHMQSGITDKPHLEEKRCTGCGMC"
BASE COUNT          291 a          340 c          443 g          273 t
ORIGIN      
        1 tgaatcgcca gaaaggacac ggggtgatca agccatgagt cgaaaaaccg aaattctcgt
       61 ggccggtggt ggtccggccg gtatgtcggc ggccattgtc gcggctcagc taggtgccaa
      121 ggtgctgctg gtcgatgaca accgcgtgct gggtggccag ttggtgaagc agacgcatcg
      181 tttcttcggc agttcggctc gtgatgcggg cattcgtggt attgcgatcg ccgacgaact
      241 gcagaagcag gtggcggcgc agaagaagca gttgacggtg ctgaccgaga ccgcggtggt
      301 cggcttttac agcgataatc aggctggaat tctgtcgcat aaagcgtacg agccggtgac
      361 ctacgagagg gccatcgtgt gcgctggagc gaccgaaaat agtctgtctt ttgaaaacaa
      421 tgatctgccg ggtatctacg gtgccggcgc ggtgcagacc ttgatgaacg ttcacggcat
      481 tcgcccggga aagaaagtgc tgatgatcgg gtcgggaaac atcggcctga tcgtgagtta
      541 tcagttgctg caggcgggcg tcgatgtggt cggcctggtc gaagcgctgc cgacgatcgg
      601 cgggtatctg gtacatgcca gcaagattcg tcgtctggga gttccgatct acacctgtca
      661 cacgatcaag caggcgatcg gtacgaatca tgtcgaagga gctgctctgg ttcgtattga
      721 cgaaaggttc caaccggtac caaacaccga gtttcaggtg gagtgcgact gcatctgtct
      781 ggccgtcggt ctctcacctc tgatcgatct ggtgcagcag ggtggcgcaa aaatggtgtt
      841 tgtaccagaa cttggcggcc tggttccgct tcatgacgga gacatgagga cgacgagccc
      901 gggagtctat gttgccggtg atgcttcggg gatcgaagag gcaacgacgg ccattcttgg
      961 cggccgtatt gccggggcgc gcgcatacga gagtctgcac ggcaagaagc ccgaggctga
     1021 agccatcgtg gtcgatgcca acaacgaact caagaccctg cgcagcggtt cgaccggtga
     1081 acggatccgg tggggccgag atcggctgct gaaggagatt gccgcatgtt gaagaacgac
     1141 ggaattccga cgcgagaaga atacgaaacc attcttccga cccctgaacg catgaagaag
     1201 gggccggttg cgatcattga gtgttttcag gatatcccct gtgacccgtg tgtgaccgcc
     1261 tgcccgacca aggctatcca catgcagagc ggcattacgg acaaaccgca cctcgaggaa
     1321 aagcgctgca ccggctgtgg tatgtgt
//
LOCUS       DAORHD010001485         1347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138868_length_1347_cov_2.474458, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001485 DAORHD010000000
VERSION     DAORHD010001485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138868_length_1347_cov_2.474458"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            550..744
                     /locus_tag="PKO06_22830"
                     /pseudo
     CDS             550..744
                     /locus_tag="PKO06_22830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174499.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
     gene            complement(865..>1347)
                     /locus_tag="PKO06_22835"
     CDS             complement(865..>1347)
                     /locus_tag="PKO06_22835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72563.1"
                     /translation="YSEVGKGTVFRLLLPVSSEVAVSRVENRVPVRGHGRLLLVDDEP
                     GIRDSGRAILTQLGYEVLLAENGADGVDVYQKEHEKIDLVLLDLAMPVMDGQTCLKKM
                     HEIDPSIPVIVCSGFARDDVLTELEPLGVEAFLIKPFRVVELSQAIAQVLVANRPPDH
                     "
BASE COUNT          305 a          360 c          395 g          287 t
ORIGIN      
        1 tggcgctgtt gtcctcaaaa atgcgtttca tggttttcct cttttactac tgcatgatag
       61 aaaggatacg gatagactac cgttatccga ggcaaacggc aactgggcag cgccaaccag
      121 gctctccgtt gtagcgtgcg cttgagatga actcagggat tcctcgcact gaatacgagc
      181 aatgcgtgcc tgattggctg acgagccaga ccggaagata acaggagaat tggcatgagg
      241 ttacccgaat ttgtcgtgac ggcgtttcat ggatttgcac aggatctcta tctcgcagcc
      301 gggcagtttg gcgaatgctg cgacagcagg acaaatcatg gggatgaatt gtccaggctc
      361 gcgactattg ccagccgcat cgaacgcatg aatgaagaca gtgaatacga tgttgaggtg
      421 ggtgaccgac tggcgtttca tccggacaaa gggctcgaac tcatcggaaa gatgcgtccc
      481 gtggcccggg atgttctgac cgcctttctc gatgagtatg aaaaatcact gcagctgatt
      541 tcgcgcgaca tgcagtgtcg gagtgcctgg caacgatttc tggaaggcgg cgactacgat
      601 tatctgaccg aacagtacac gtttgaaaaa agtggtccgc tctcctgttc gttttgcggc
      661 aagacgcagg ccgaagttcg caagctcatt gccgggccgt cggtcgccat ctgtgacgaa
      721 tgtgtcacac tgtgcaacga gatcacggcc gcgggataag cagaaacgtt cgcgggcatt
      781 tgcaacaaac cgctgccagc cacggcagtg gctgcttggc gtagcgacgg gttggataga
      841 cccgcgagcc aggagggctg ggtgtcagtg gtcgggtggg cgattggcga cgagaacctg
      901 ggcgatggcc tggctgagtt caacgacacg gaacggcttg atcagaaagg cctcgacgcc
      961 cagcggttcg agttccgtga gcacatcatc tcgcgcgaac ccagaacaca cgatgacggg
     1021 aatgctcggg tcgatctcgt gcatcttctt gaggcaggtt tgcccatcca tgaccggcat
     1081 ggccagatca agaagaacca ggtcgatctt ttcgtgttcc ttctgataca cgtcgacccc
     1141 gtcagcgccg ttttcggcca gcagcacttc ataaccgagc tgtgtcaaaa tggctctgcc
     1201 ggaatcgcga attccgggct catcatcgac gagcagcagt cgcccgtgac cgcgaaccgg
     1261 aactcggttc tccactcggc tgactgcgac ctcgctcgag actggcaaca agagacgaaa
     1321 gaccgttccc ttcccaactt cgctgta
//
LOCUS       DAORHD010001486         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_138997_length_1346_cov_3.317583, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001486 DAORHD010000000
VERSION     DAORHD010001486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138997_length_1346_cov_3.317583"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="PKO06_22840"
     CDS             complement(<1..677)
                     /locus_tag="PKO06_22840"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNV72564.1"
                     /translation="MVRKTVIGFMVLCLVVLGVLPVSAYEVVKPEEVDAGMPYEVKQV
                     PVENPLALHGLKVALVAAHGFEEVEATYPIEFLVARGAVVDVITPDWIKDRVMAVRFL
                     KPSVWIPVTKQISQARMEDYAAIIVPGGAWNPIIMRTDTAILDFVRRAHSAGVLVASI
                     CHGPQVLLSAGLLSGKEVTGVGDIRQDLRNAGAHVHEDRPVVIDGNLLTSRDPHDLPD
                     FATAIEQ"
     gene            878..>1346
                     /locus_tag="PKO06_22845"
     CDS             878..>1346
                     /locus_tag="PKO06_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HNV72565.1"
                     /translation="MKRSVLWVLWVFTMLSLSVSAAVQEKVVEYSLDGTALEGVLVYD
                     DAVTDKRPGILVIHDWMGVGPYVKSRCRQLAELGYVAFAADIYGKGVRPANAQEAAKQ
                     AGIYKKDRALMRARARAGLAELQKQPHVDANSVAAIGYCFGGTTVIELARSGAD"
BASE COUNT          294 a          385 c          385 g          282 t
ORIGIN      
        1 cactgctcga tggccgtcgc aaaatccggc agatcatgcg ggtcacggct ggtgagcaga
       61 ttgccatcga tcaccacggg acggtcttcg tgcacgtggg ctcctgcatt gcgcaggtcc
      121 tgacgaatgt cacccacacc cgtgacttcc tttcccgaaa gtaacccggc gctgagcagc
      181 acctgagggc cgtgacagat cgaggccacc aggaccccgg ccgaatgggc ccggcgcacg
      241 aaatcgagaa ttgccgtatc cgtgcgcatg atgatcgggt tccaggcacc accaggaacg
      301 ataatcgccg cgtaatcttc catgcgagcc tgcgaaatct gtttcgtcac gggaatccag
      361 acgctgggct tcaggaatcg gacagccatg acccggtctt tgatccaatc cggcgtgata
      421 acgtcaacca cggctcctct cgccaccaga aactcgatcg gatacgtcgc ctcgacttct
      481 tcaaagccgt gagcggcgac gagagccacc ttgaggccgt gcagagcgag cgggttctcg
      541 accggaacct gtttcacctc atagggcatg ccggcgtcca cttcctcagg tttcacgacc
      601 tcgtatgctg ataccgggag aacgccaaga accaccaggc agagaaccat gaaaccgata
      661 actgtctttc gtaccatgtg aagtacctcc tgaaatgttg tgtgtgagca catcagcact
      721 cattcacgaa ctcgattgta gccgggggcg tgccgaaaaa cttttcattc cttgccattc
      781 tggctaaggc tcccaaaaag tcgttcttgt tgcacaaccg atattgatat atatttaggg
      841 tcgtatgaac ttcgaaaatc actcaggagg aaatgatatg aagcgctctg ttctttgggt
      901 gctctgggtt ttcacgatgc tcagcctcag cgtctcggct gcggtgcagg aaaaggtcgt
      961 cgagtattcg cttgacggca cggcactcga aggagtgctc gtgtacgatg acgctgtcac
     1021 cgacaagcgc cccggtattc tggtcattca tgactggatg ggcgtcggac cgtatgtgaa
     1081 aagtcgttgt cggcagttag ccgagctcgg ctatgtggct tttgcggctg acatctacgg
     1141 caagggtgtc aggccggcaa acgctcagga agcagcaaag caggccggta tctacaagaa
     1201 agatcgcgcc ctgatgcgtg cacgcgcccg ggccgggctg gctgagctgc agaagcaacc
     1261 gcacgttgac gcgaactccg tcgcggcgat cgggtattgt tttggtggca ccacggtcat
     1321 tgaactcgcc cgcagcggtg ccgacg
//
LOCUS       DAORHD010001487         1346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139022_length_1346_cov_2.801704, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001487 DAORHD010000000
VERSION     DAORHD010001487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139022_length_1346_cov_2.801704"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PKO06_22850"
     CDS             <1..900
                     /locus_tag="PKO06_22850"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72566.1"
                     /translation="HFGPPFLLSVDRLLARIRNLTVRAMPSGTLFPQEMSQYDTWVIR
                     EALHNCIAHQDYHLRGRITVVETPDSLMFTNVGSFLPGNVEQVIRQDAPPDIYRNPFL
                     AEAMVNLNMIDTQGGGIKRMFRMQIQRFFPLPDYDLTQPERVAVRLTGRIIDEQYTQL
                     LMQRGDITLEQVMLLDRVQKRLPIPREAHQKLKSAGLIEGRYPNSMVSSLVARVTSQK
                     ARHIRERGLDKQYYLDMILALVKEHGPVTREDIDQLLSDKLPDILTPQQKAKKVHNLL
                     CELSRKKQIVNKGSRRISQWFMC"
BASE COUNT          332 a          372 c          347 g          295 t
ORIGIN      
        1 cacttcgggc cgccctttct gctttcggta gaccgcctgc tggcgagaat ccgtaatctg
       61 accgtacgcg ccatgccaag cggaaccctg tttccgcagg aaatgagcca gtatgacacc
      121 tgggtgattc gtgaggctct acacaactgc atcgcccacc aggattacca tctgcgcggg
      181 cgcatcaccg tcgtcgaaac gccagattcc ctgatgttca ccaacgtcgg ttcgtttctg
      241 ccgggcaatg tcgaacaggt gatcagacag gatgcgcctc cggacatcta tcgcaacccg
      301 ttcctagctg aagccatggt caatctgaac atgatcgaca cccagggcgg aggcatcaaa
      361 cgcatgttcc gcatgcagat tcagcgcttt tttccacttc ccgactacga cctgactcaa
      421 ccggagcgag tcgccgtgcg gttaacagga cgaatcatcg acgagcagta tacgcaactg
      481 ctcatgcaga ggggcgatat caccctggaa caggtcatgt tgctggaccg ggtgcagaag
      541 cgcctgccta ttcctcgtga ggctcaccag aagctgaaaa gcgccggtct cattgaaggc
      601 cggtatccga actcgatggt cagtagcctc gtggcacgcg ttacgtctca gaaggcgcga
      661 cacattcgcg aacgaggtct ggacaagcag tattatctcg acatgatcct cgcgctggtc
      721 aaagaacacg gccccgtcac ccgtgaagat atcgaccaac tactttcaga caagctcccc
      781 gacattctca cgccgcaaca gaaggccaag aaagttcata accttctctg cgaactgtct
      841 cgtaaaaagc agatcgtcaa caagggaagt cgtagaattt cacagtggtt tatgtgttaa
      901 aaatgaaacg tgatagtatt aagcaaaact tagcaaagaa aagcaaatga gctttttcct
      961 gcgagatgcg aaagtccgct tccagaaagg cttttcattt gctaggcgtg cggcggaatc
     1021 aaacaataat gtttgtcctg cccaggttga gtttatttga tggtcatttg atgacggagg
     1081 tttctcccca ctcgacatgc gtgggaacag gttgaaaatg agcttcaaca gcgagctttt
     1141 tgcgtttgcg tcttatttga tggtcatttg atgactgcct gttcagggag aaaccagcgg
     1201 ccatgacacc taccgatacc agcgaaaaag ggctcgaaag cctcattgtc accgagatga
     1261 ccgggcgacc ctggccggac ccgactcttt ccacgacttg caccgattcc ggcgccgggt
     1321 tcggcgggac cggctggatc aacagc
//
LOCUS       DAORHD010001488         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139171_length_1345_cov_3.234884, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001488 DAORHD010000000
VERSION     DAORHD010001488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139171_length_1345_cov_3.234884"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..833
                     /locus_tag="PKO06_22855"
     CDS             33..833
                     /locus_tag="PKO06_22855"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72567.1"
                     /translation="MTSRGCPYKCVFCHNSWRPSPVRFHSPEYVIREIRNLYHAYQVQ
                     ALFFFDDNFLSRKSRVLEICELLRQERLPIIWGCQARAEQVSEELLRAIYAVGCRQVT
                     FGLESGSQRVLDSLKTGGVTLEQNRRAVELCHRLGFKTWGTFMIGSPGETYEDIKATR
                     RFMRSLPLSGAMVHVTTAFPGTPLWSQVVERGQTAADPDWKNFTTADASVELGLFSKN
                     TLRKIRRKLMLAEVIFQGKFDWRSIMQLCLRNPKQVLTRFLKILVTDR"
BASE COUNT          285 a          339 c          361 g          360 t
ORIGIN      
        1 tcgttcctaa cggttttcgg acggctgcta tcatgacctc tcgggggtgt ccgtacaagt
       61 gtgtgttctg tcataacagt tggagaccgt cgcctgtgcg atttcattca cctgaatatg
      121 tcatcagaga gatccggaat ctgtatcacg cgtatcaggt gcaggcgctt ttctttttcg
      181 atgataactt tttatctcga aagtcacgtg ttctcgagat ctgtgaattg ttgcgccagg
      241 aacgcctgcc gatcatctgg ggatgccaag ctcgcgctga gcaggtcagc gaggaactgt
      301 tgcgagctat ctatgctgtg ggttgtcgcc aggtcacgtt tgggcttgag agtgggtctc
      361 aaagagtcct tgattcgctg aagaccggag gggtcacgtt ggaacagaac cggcgggctg
      421 tcgaactgtg tcaccgcctg gggttcaaaa cgtggggaac attcatgatt ggttctccgg
      481 gagagactta tgaagacatc aaagcgacgc gccggtttat gagatcactt cctctcagcg
      541 gtgccatggt tcacgtgacg acggcgtttc cgggtactcc tttgtggtct caagtagtag
      601 aaaggggcca gactgcggcc gacccggact ggaagaactt taccactgcg gacgcgtcgg
      661 tggaactggg cttgttctcg aagaacaccc tgcggaaaat tcgccgtaaa ctgatgcttg
      721 ccgaggtgat ttttcaaggg aaattcgact ggcgctcgat tatgcaactc tgtcttcgaa
      781 atccgaaaca ggtgctgaca aggttcctga aaatccttgt gactgatcgt tgaactctcg
      841 tgtgctatga gatttgacac ctcactgatt ctcgtcgagc ccccctcgga tgtgaatctc
      901 aacctcccta atctcgatct tgcttatgct gctacccttc tgagaactcg agtcattgat
      961 tatcacgcct gtccattccc ctcacaacgt ttgctgcgaa aggaatggcg gcaggtggtg
     1021 atatctcagc gcagcttcaa taaatctgca gctgctgaat tgtctcaggc atatgaacgc
     1081 aagtatccgg cgagtgtcgt ccggggattg catggagtcg tcgacattca gtgctgctat
     1141 ccatttcttc gcagtacatc ggatcttcgc gtgactgaaa agttcggaga ttctctccca
     1201 ttccccgagt atcaaagatt cgactcgtac aattacctgc gcgcaaattg gtcgtccgga
     1261 tggtggggct accctattct cacgagcttg ggatgcccct ttacctgtgc attctgtcat
     1321 gcacggcaac gatcatggcg tccac
//
LOCUS       DAORHD010001489         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139177_length_1345_cov_3.130233, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001489 DAORHD010000000
VERSION     DAORHD010001489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139177_length_1345_cov_3.130233"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /gene="fusA"
                     /locus_tag="PKO06_22860"
     CDS             <1..272
                     /gene="fusA"
                     /locus_tag="PKO06_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276850.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV72568.1"
                     /translation="DSYMGTIIGDLNSRRGRIMGMEPLGDGNQLVKATVPLAEMYKYA
                     TDLKSMTQSRADFSMKFDHYEEVPGNVTEQIVKEYGRKTEDEVEE"
     gene            complement(349..>1345)
                     /locus_tag="PKO06_22865"
     CDS             complement(349..>1345)
                     /locus_tag="PKO06_22865"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72569.1"
                     /translation="IAEPFWKFPDFDPYSLNQAVKQRIIDLQRMVSGVVNDVVAFVPV
                     ISDGRMTGGLGLQKPVTQDQLQPKMYLLHALGLMIGNGFSNSMTRGALERRIDQAHLE
                     TAKVQQQQDSLKQAFSRYVSSSVVDEISRNAGSINVGGKRQAITILFADLRGFTALAS
                     KMPNEGLAVLLNDWLERASQRLFQLDGTIDKFLGDGIMALFGAPLPQADHALRAAYTA
                     FNLQEAFENYLSENRKILEDNTIGMGIGIATGTVLVGNFGSRRRMEFTAVGDAVNLAA
                     RLEKVAEDGDIIIDKTTLNLLQDRFAFTTKSKVPLKGKEPMDVYRLTGVKVPWGS"
BASE COUNT          299 a          408 c          342 g          296 t
ORIGIN      
        1 cagattccta catgggcacc atcatcggcg acctcaacag ccgccgcggc cgcatcatgg
       61 gcatggaacc cctgggcgac gggaaccagc tcgtcaaggc cactgtgcct ctggctgaaa
      121 tgtacaagta tgcgaccgac ctcaagtcga tgacccagtc tcgcgccgac ttctcgatga
      181 aattcgacca ctatgaagaa gtgccgggca acgtcaccga gcagatcgtc aaggaatacg
      241 gccgcaaaac tgaagacgaa gtcgaagaat aagacaccaa gaaaaacccc tgctcgcttg
      301 gcgggcaggg gtttttcttt gatgattttc cgttcgacgg cctgaatctc agcttcccca
      361 aggcaccttc acaccggtca accggtacac gtccatgggt tctttgccct tcaacggcac
      421 tttgctcttc gtagtaaacg caaatcggtc ctgcaacaga ttgagcgtcg ttttgtcgat
      481 gataatgtca ccgtcttcgg caactttttc gaggcgtgcc gccaggttca ccgcatcgcc
      541 cacggccgtg aattccatgc ggcggcgcga cccgaagttg ccgaccagca ccgtaccggt
      601 tgcgatcccg attcccatac cgatcgtgtt gtcctcgaga atcttgcggt tttcactgag
      661 atagttttca aaagcctcct gcaggttgaa cgccgtatag gcggctctca acgcgtgatc
      721 agcctgcggc aggggagctc cgaaaagcgc catgatgcca tcgcccagga acttatcgat
      781 cgttccgtca agttgaaaca gtctctgact ggctcgttca agccagtcgt tcagaagcac
      841 cgccaacccc tcgttgggca tctttgaagc caacgccgtg aacccgcgca ggtcagcaaa
      901 cagaatggta atggcttgac gcttgccgcc aacgttgatg ctcccggcgt tacgcgaaat
      961 ttcgtcgacc acgctcgatg agacataccg ggagaaagcc tgtttcagcg aatcctgctg
     1021 ctgctgcact tttgccgttt ccaggtgtgc ctgatcaatt ctgcgctcca acgctccgcg
     1081 agtcatacta ttcgaaaagc cgtttccgat catgagtccg agcgcgtgca acaggtacat
     1141 tttcggctgc agctgatcct gagtcaccgg tttctgcaaa ccgagacctc cggtcatcct
     1201 gccatcgctg atcaccggca cgaaggcgac cacgtcattg acgacgcccg acaccattcg
     1261 ctgcaggtcg atgattcgct gtttcacggc ttgattcagg ctgtatggat cgaaatccgg
     1321 aaacttccag aatggctccg caatc
//
LOCUS       DAORHD010001490         1345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139243_length_1345_cov_2.260465, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001490 DAORHD010000000
VERSION     DAORHD010001490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139243_length_1345_cov_2.260465"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKO06_22870"
     CDS             complement(<1..318)
                     /locus_tag="PKO06_22870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72570.1"
                     /translation="MPGAVITTRSPRFTPRFHRGIVLAILLFLPVFLVSGAGISRFQE
                     YPDTLARQARRTLPVWSPTLIPELSWFLADRFDHHRALVLPPLKPLTSETAANDSAAA
                     GITI"
     gene            479..>1345
                     /locus_tag="PKO06_22875"
     CDS             479..>1345
                     /locus_tag="PKO06_22875"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV72571.1"
                     /translation="MMSNALDTRLADMSDADRDRYLRGKYRELQLINRLDRITDRYSR
                     LPRLMEKSLALMQSFFGVSVAAVQLTSAEQLENRGFYYTGPADASEALQSAARDLLAQ
                     FLSEPQVEIRQRHESGPYAQSGLTNTLIIPMVITSEIAGAFLLANRDEAFSESDVHFL
                     KVFSSQLDNAVIHARVLDDYRKTARDLKQRVRQLNVLYEMSLSLGLGFDFESLAKRII
                     ESTQQIVPFDRCSIMLYDKKSDDLKTEVVVGEKQKIRLVHLSRGKGIAGLALLSKRPI
                     LAEHGSEDERFVP"
BASE COUNT          319 a          352 c          402 g          272 t
ORIGIN      
        1 gatcgttatt cctgctgccg ctgaatcatt ggccgcagtc tcagaggtca ggggcttgag
       61 cggcggaagc accagggcgc gatgatggtc gaatcgatcg gccagaaacc acgacaactc
      121 cggaatgagc gtgggtgacc agacaggcag agttcgccgg gcctgcctgg cgagggtgtc
      181 aggatactcc tgaaaacgcg aaatgcccgc acctgagacc agaaaaaccg gcaggaagag
      241 caggatggca aggacgattc ccctgtgaaa acggggggtg aaacggggac tccgtgtggt
      301 tatgaccgcg ccgggcattg gtgttgttgt ttctaccttt tctggtctgg aaagaagtaa
      361 agataccgaa aaaagagcat actctctctc cgggaaatgt tccagaagtg aattgaccgg
      421 aaccgcaccg gcgagtatcc tacgcacgag agacagcatg accagcggag ggcctgagat
      481 gatgagcaac gcactggata cacgacttgc tgacatgtca gacgcggatc gagaccggta
      541 cctgcgtgga aaatatcgcg aactgcagtt gatcaatcgg ctcgatcgca tcaccgatcg
      601 gtacagtcgc ctgcctcgcc tgatggaaaa gtcgctggcc ctgatgcagt cgttctttgg
      661 tgtcagtgtc gcggcggtgc agttaaccag tgccgaacaa ctcgagaacc gcggttttta
      721 ctacaccggc ccggccgacg cttcagaagc cttgcagagt gcagcccgtg atctgctggc
      781 gcagtttctc agtgaaccgc aggtggagat ccgacagcgg cacgagagcg gtccgtatgc
      841 gcagtccggg ctgaccaata cgctcatcat tcccatggtc attacatccg agattgccgg
      901 agctttcctg ctggcaaatc gcgacgaagc cttttccgaa tcagatgtgc actttctgaa
      961 ggtcttcagt tcgcaactcg acaacgcggt cattcacgcg cgcgtactcg atgattaccg
     1021 caaaaccgct cgcgatctga aacaacgcgt gcgccagctg aacgtcctct atgaaatgag
     1081 tctcagtctg ggattaggct ttgacttcga gagcctcgcc aaacgtatca tcgagagtac
     1141 gcagcagatt gtcccatttg atcgctgttc aatcatgctc tacgacaaga agagcgatga
     1201 tctgaaaacg gaggtggtgg ttggcgagaa gcagaagatt cgattggtgc atttgtcgcg
     1261 cgggaaggga attgccggac tggccctgct gagcaaacgg ccgattctgg cggagcacgg
     1321 tagcgaggac gaacgtttcg tgccc
//
LOCUS       DAORHD010001491         1344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139394_length_1344_cov_2.681924, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001491 DAORHD010000000
VERSION     DAORHD010001491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139394_length_1344_cov_2.681924"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKO06_22880"
     CDS             <1..628
                     /locus_tag="PKO06_22880"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV72572.1"
                     /translation="FTSMFLHGGWFHFLVNMWSLYLFGDDVEDRLGSTRYLTLYLLSG
                     LIAGLSHALLFGDSTIPTIGASGAIAGIMGAWLTLFPRARIDVLVPLFIFLQIWTIPA
                     AFYLPVWFLSQLLSGTLALAAPGFSGVAFWAHIGGFVAGMFLTPWLSQLATDRVSQSM
                     PKPERVEPAHARSARDTSTGAARRVRVVFPDAIVQGEQVYVFRPLHQR"
     gene            778..1338
                     /locus_tag="PKO06_22885"
     CDS             778..1338
                     /locus_tag="PKO06_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015348042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV72573.1"
                     /translation="MDYLEVTPHCWPLVEKLFGAKGASGGCWCQYWRIAKGEKWEAIK
                     GEATRQRLHDGIANGSMHGALALENGEPIGWCTFGPRRSFSRLDRAPSFTCDDADEVW
                     SIPCYFIKPGFRGKGVATGLLSFVIQLLQARHVRVLEGYPSKPGADGKYVAAFAWTGT
                     QGLFAKLGFTLAGNADGGKQRVRRTL"
BASE COUNT          219 a          429 c          392 g          304 t
ORIGIN      
        1 gttcaccagt atgttccttc acgggggctg gttccatttt ctggtgaaca tgtggtcgct
       61 gtatctcttc ggcgacgacg tcgaagaccg cctcgggagc actcgctatc tgactctgta
      121 ccttctgtcc ggactgatcg ccggcctctc gcacgctctc ctgttcggcg actccacgat
      181 tccaacgatc ggcgcatcag gggcgatcgc cggcatcatg ggtgcctggc tcaccctgtt
      241 tccccgcgcc cgcatcgatg tgcttgttcc gctgttcatt tttcttcaga tctggaccat
      301 ccccgcggcg ttctatctgc cggtgtggtt tctgtcgcag ctcctctcgg gaactctggc
      361 tctggccgct ccaggcttct ctggtgttgc gttctgggca cacatcggcg gtttcgtggc
      421 cggcatgttc ctgacaccct ggctttcgca gctcgccact gaccgcgttt cgcagtccat
      481 gccgaagccg gaacgagtcg agccggcgca tgcccgctca gcccgtgaca cgagcacagg
      541 cgcagcccgc cgcgtgcgag tggtgtttcc tgacgcgatc gtgcagggcg aacaggtcta
      601 cgtcttccgc ccgctccacc aacgttgagc agacatgcct ctccatcagc tccctcctct
      661 ccctcgccgc ctcccgacac agaggcggtt ttcttttctc tcccttgcca atcgagtgct
      721 tggtcgacaa agtgtggtat cttcctggct ggtactttgg atgctcagga gttcatcatg
      781 gattacctcg aagtcacacc gcactgctgg cccctggtgg aaaaactgtt cggcgcgaaa
      841 ggtgccagtg gcggctgttg gtgccagtat tggcgcatcg ccaaaggcga gaagtgggaa
      901 gccatcaaag gcgaggccac cagacagaga ctgcacgacg gtatcgccaa cggcagcatg
      961 cacggcgccc tggcactgga gaatggggaa cccatcggct ggtgcacctt cgggccgcgc
     1021 cgcagttttt cacggctcga ccgcgccccc agttttacct gcgacgatgc cgatgaggtc
     1081 tggtcgattc cctgttactt catcaaacca ggctttcgcg gaaaaggcgt ggccaccggc
     1141 ttgctttcct ttgtgattca gctgttgcag gcacgtcacg tgcgcgttct cgaagggtat
     1201 ccgagcaagc cgggggcgga cggaaagtat gttgccgcgt tcgcctggac cggcacccag
     1261 ggtctcttcg ccaaactcgg gtttacgctg gccggcaacg ccgacggtgg aaagcagcgg
     1321 gtcagaagaa ctctgtgacc agca
//
LOCUS       DAORHD010001492         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139781_length_1342_cov_2.637141, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001492 DAORHD010000000
VERSION     DAORHD010001492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139781_length_1342_cov_2.637141"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PKO06_22890"
     CDS             complement(<1..337)
                     /locus_tag="PKO06_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72574.1"
                     /translation="MTVPPASSWWWNVVLRGFVILAFIVLPAFVYWYISFLETVTTAD
                     HEREFLRQQLLAQAQEVPEQIAHERVIGSFLYRALRILGRTGKTGVRDMLERMERDYP
                     GVFQWAFWDH"
     gene            624..>1342
                     /locus_tag="PKO06_22895"
     CDS             624..>1342
                     /locus_tag="PKO06_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72575.1"
                     /translation="MADMRQTFVFDFALRRTLRRFAALSGFAVCLCAFALLGSGCGAG
                     GSGGSGQRASDWVGLTGPVIEGAAPTLTIMPATQTLTTGRQMQVLVIGRNEYGRPLAD
                     GVKLQLSSQLGTTAESAEISTTGGMALFLITGGPSAGLETLTVSGAGVFTTATLQLVA
                     EAIEIPQISISPASDTVKPSGVLPIVVFVRNATGAPLATDVTLLSDLGGTFDKNTGTS
                     AGGYFVSYYTAPVDPGQDVITV"
BASE COUNT          294 a          402 c          387 g          259 t
ORIGIN      
        1 ggtgatccca gaaagcccac tgaaacaccc cgggataatc gcgttccatg cgttcaagca
       61 tatcgcgaac cccggtcttt cccgtgcgcc cgagtatgcg taaagcccga tacaaaaagg
      121 agccaatgac gcgttcgtgc gcaatctgtt cgggaacttc ctgtgcctga gccagaagct
      181 gctggcgcag aaactccctt tcatggtcgg ccgtggtgac cgtttcgaga aatgagatgt
      241 accagtaaac gaatgccgga agtacgatga atgcaagaat gacaaaccca cgcaacacga
      301 cgttccacca ccaggaagaa gcggggggca cggtcattcg agcaaccgta agggaatcgg
      361 tcgatacggg cacgttcgca ctctccctct cgtgaatacc agctcgacta ttgtatcgcg
      421 agtgactcga agcgccaatc tcgaaaagcg aaaggaaatc ctgtctcttc gtctcaaaac
      481 gaatttcccc gcgaaaaaac ttgtctgtac ttacggctgc cggtatagtt ttcgcacacg
      541 gtgcggtaca cgcactgaca agagatgaga tattcagaag gtgggcctcc agaggcctgc
      601 cgacaggaga ccagggagat cccatggcag atatgcgtca gacattcgtt ttcgatttcg
      661 ccctgcggcg taccctgcga agattcgccg cactctccgg attcgccgtt tgcctgtgcg
      721 ccttcgcact gctgggctcg ggttgcgggg caggtggttc gggtggcagc ggtcagcgtg
      781 ccagtgactg ggtcggtctg acgggtccgg tcatcgaagg cgcagcgcca accctgacga
      841 tcatgcctgc cacacagacg ctcacgaccg gccggcagat gcaggttctg gtcattggtc
      901 gcaacgagta tggtcggcca ctggccgacg gtgtaaaact gcagctcagc agccaacttg
      961 gaacgacggc cgagtcagcg gagatctcca ccacaggtgg catggcactg tttctgatca
     1021 ccggcggccc tagtgccggg ctcgaaaccc tgacggtcag cggtgccgga gtcttcacta
     1081 ccgcgaccct tcaactcgtg gccgaagcca tcgagattcc gcagatctcg atttcgccgg
     1141 catctgacac cgtcaagccc tcaggggtgc tgcccatcgt ggtcttcgtg cgaaacgcca
     1201 ccggcgcccc gctggcgacg gacgtcacac tactgtctga tctgggcgga accttcgata
     1261 agaacacggg aaccagtgcc ggcggctact tcgtgtcgta ctacaccgct ccggtagacc
     1321 cagggcagga cgtgatcacc gt
//
LOCUS       DAORHD010001493         1342 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_139794_length_1342_cov_2.498057, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001493 DAORHD010000000
VERSION     DAORHD010001493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139794_length_1342_cov_2.498057"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PKO06_22900"
     CDS             <1..329
                     /locus_tag="PKO06_22900"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNV72576.1"
                     /translation="QKKPLSKIISDLYAKVGTYLTRRLNFKLTQAEKDRLTEKVKADP
                     TEFGGMKVTKVIRVDGTKFLLENDAWILIRMSGTEPVVRFYAEAKEEKTLDQLCKHGE
                     NWVRNA"
     gene            474..809
                     /locus_tag="PKO06_22905"
     CDS             474..809
                     /locus_tag="PKO06_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72577.1"
                     /translation="MTITPIDIKTSLVVNTDASRLRENQKAQESGPGQQLVGLQQQKD
                     QQKTETLQQPQATEDHLIRKEDQDSEKRGNQQEAAPGAKPSQPAEEKKPAEPVPDPAG
                     RGWNIDVKA"
     gene            917..>1342
                     /locus_tag="PKO06_22910"
     CDS             917..>1342
                     /locus_tag="PKO06_22910"
                     /inference="COORDINATES: protein motif:HMM:NF024574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72578.1"
                     /translation="MAEDDARLRNRQGVLHAQRGDLLTAIRSFERARLADPLDDTALT
                     NLSCAHNNMGVLLCRDRRFTEAIAHFEKAKQHKPEDLQIRFNLLSALVLIRDAERVDI
                     EARGILALRPRDPETINKVANAFLKIEDDDSARNLLERIL"
BASE COUNT          332 a          406 c          351 g          253 t
ORIGIN      
        1 tgcagaagaa accgctctcg aagatcattt ccgacttgta tgccaaagtg ggcacctacc
       61 tcacccgtcg cctgaacttc aagctgaccc aggccgagaa agaccgatta accgagaaag
      121 tgaaggccga tcccacagag ttcggcggta tgaaagtcac caaagtcatt cgggttgatg
      181 gcaccaagtt cctgctcgaa aatgacgcct ggatcctgat ccgcatgtcc ggaaccgaac
      241 ccgtcgtgcg tttctacgca gaagccaaag aagaaaagac tctcgaccag ctgtgcaaac
      301 acggcgaaaa ctgggtgcgc aacgcctgaa accgcttctc cagcgggcac ggaacctcat
      361 gattccgtgc ccgcttgctt ttctgggcgg gtcaccgccg gcaaaaaagc tcagattcaa
      421 gcttccttgc gtcgatgagt actttaggta cctttgaagt agcgggtaac cctatgacta
      481 ttacaccgat cgatatcaag accagcctgg tagtcaacac cgacgccagt cgtctgcgcg
      541 agaatcagaa agcgcaggaa agcggccccg gtcagcagct cgtcggcctg cagcagcaaa
      601 aagatcagca gaagacagaa acgctgcaac agcctcaggc gaccgaagat cacctgatcc
      661 gcaaggaaga ccaggattca gaaaagcgcg gcaaccagca ggaagccgct cccggcgcga
      721 agccctctca gcccgccgaa gagaagaagc cggctgaacc tgttcctgac cccgctgggc
      781 gcggttggaa catcgacgtc aaagcctgat ggtcctgacc gccgctcgtt cctgccactc
      841 tgatttctgc aggcgtgttc atgcctgttt attcaccatt tttctgctct cgctctcggg
      901 cgtgggcgcg ttcggcatgg ccgaagacga tgcccgcctg cgaaaccgcc agggagtgct
      961 gcatgcccag cgtggtgacc tgctgaccgc catccggtca ttcgaacggg cacgattggc
     1021 cgaccctctc gatgacacgg cactgaccaa cctttcctgc gctcacaaca acatgggggt
     1081 gctgctctgc cgcgatcgcc gattcacgga agctattgcg cactttgaaa aagccaaaca
     1141 gcacaaaccc gaagatctcc agatcagatt caatctgctt tcagctctgg tactgattcg
     1201 agacgccgaa cgggtcgata tcgaagctcg gggcattctg gcactgagac ctcgtgaccc
     1261 cgagaccatc aacaaagtgg caaatgcgtt tctgaagatt gaagacgatg attccgcccg
     1321 caatctgctc gaacgaattc tc
//
LOCUS       DAORHD010001494         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_140017_length_1341_cov_2.053655, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001494 DAORHD010000000
VERSION     DAORHD010001494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140017_length_1341_cov_2.053655"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..>1341
                     /locus_tag="PKO06_22915"
     CDS             213..>1341
                     /locus_tag="PKO06_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72579.1"
                     /translation="MNGKKLVFLLTIVALFLSGAVFAQAKRAPSNDMGLVDDIPEPPD
                     ASAELPSPIPMVVEYVGGLMAGDFDKCLQHFHVPTFLGLIFGDDVKVMQDADYRELYS
                     YQIQAQRNEFRFMSKVMNRVAKGCKYAYSNPRYHQNVQSKVVVKMQTTKGPHEFVVFS
                     RYVDERWQVYDYSLDGKRFTDSFRAGLNGMKPMDYTAMLRPFYGDQKKFKQVQNDDFG
                     FSMKLPENFQIREKVSPTLLFTAQSFDNQFLMHVQAAVYPQAKGLKEVAAEIKQGLMQ
                     FNPRLYDQWRTDIAGVEIGNVVFRFDKNGKTLFTQMVIVPLGQKLVVLNFYHNSLPLM
                     KNLSNIRDRILESLKLTKIELAAASDVTIPADDTSLPSNSPN"
BASE COUNT          303 a          379 c          361 g          298 t
ORIGIN      
        1 ctcgagtcca tcgccatcgt ctcccgcatg aaggaggagc acggcatcga catcccgttc
       61 ccgcaacgtg tggtgacgct tcaggcggat ccggggctgg ttgcgttcct gtcgagaaag
      121 acctgacgaa atccgtacgc gcatttcgac gttagcctga atggaacggc atctcgtttg
      181 acattccccg aagggtgcgg taccctcaga ctatgaatgg gaaaaaactg gtatttctgc
      241 tgaccattgt ggctttgttc ctgtccgggg cggtgtttgc gcaagccaag cgagcgccgt
      301 caaacgacat ggggctggtc gatgacattc ctgaaccccc cgacgcatcg gcggagctcc
      361 cctccccgat tcccatggtg gtcgagtatg tcggcggcct gatggcaggc gactttgaca
      421 agtgtctgca gcatttccac gtgccgacat ttctcgggct catttttggc gacgatgtca
      481 aagtcatgca ggacgcggat tatcgcgaac tgtattccta ccagatacag gcccagcgca
      541 acgagtttcg cttcatgtca aaggtcatga accgggtggc caagggctgc aaatatgcct
      601 attcgaaccc ccgttaccat cagaacgtgc agtcgaaggt cgtggtcaag atgcagacca
      661 ccaaagggcc gcacgagttc gtggtctttt cgcgctatgt cgacgagcgc tggcaggtgt
      721 acgattacag cctcgacgga aaacggttca ccgacagttt ccgtgccggc ctcaacggca
      781 tgaagcccat ggattatacc gccatgttgc gcccattcta cggcgatcag aagaaattca
      841 aacaggtaca aaacgatgac ttcggcttca gcatgaagct gcctgagaat tttcagattc
      901 gcgaaaaagt ctcccccact ctgctgttca cggcccagtc attcgataac cagtttctca
      961 tgcatgtgca ggcagccgtg taccctcagg ccaagggtct gaaagaagtt gcggccgaga
     1021 tcaaacaggg gctgatgcag ttcaatcccc ggctctacga tcagtggcgc acggatattg
     1081 ccggggtcga gatcggcaac gtcgtgttcc gcttcgacaa gaatggcaag actctgttca
     1141 cgcagatggt cattgttccg cttggccaga agctggtcgt gctgaacttc tatcacaact
     1201 ctctgcctct catgaagaac ctgtcgaaca tccgtgaccg gattctcgaa agcctgaaac
     1261 tgacgaaaat cgagctggcc gcggcatcag atgtcaccat tccagccgat gacacttcgc
     1321 tgccttcgaa ttcaccgaat g
//
LOCUS       DAORHD010001495         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_140487_length_1338_cov_2.515978, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001495 DAORHD010000000
VERSION     DAORHD010001495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140487_length_1338_cov_2.515978"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKO06_22920"
     CDS             <1..326
                     /locus_tag="PKO06_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72580.1"
                     /translation="CENEAFVSHVCLAHGIRVSVLWSRDWFGGRTYGTWLANVLDRLR
                     TRKVFADWLCIPGKRGEPSNRVVEETYPDLMTPSRVTLVKNEDRRWIDEGAHGWIEEY
                     EVRTT"
     gene            complement(346..639)
                     /locus_tag="PKO06_22925"
     CDS             complement(346..639)
                     /locus_tag="PKO06_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72581.1"
                     /translation="MGQANLESENSLVVVLREDRYETQFGDGYFAYFDAVFKTPESAA
                     AYVTTKTTKDTAYHLRNIRLIVRQDRIFIEGTLAENEQITPAQVVKALASDHQ"
     gene            complement(654..>1338)
                     /locus_tag="PKO06_22930"
     CDS             complement(654..>1338)
                     /locus_tag="PKO06_22930"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNV72582.1"
                     /translation="PPTKNPDVIPAEIEIQYPELSSSLKADTAVTGINSAIQTRLLAL
                     ADGEPAKTIDELIERFAAGYAASVKETPEMPGAWSLKCVTTIRHADADLLALETITSI
                     FTGGAHPNSEITYQVYSLKTGKPIELSALIAVDKIDELNRIAEKIFRQVRNLKPDETY
                     EQAGFQFDKGRFALNNNFLVSKSGLTFCYNAYEIAPYALGTTELVIPWPDLKNVVAPK
                     SLAERFLKE"
BASE COUNT          263 a          338 c          413 g          324 t
ORIGIN      
        1 agtgtgaaaa tgaggcgttt gtgtcgcacg tgtgtctggc acatggcatt cgagtttcag
       61 ttctctggtc gcgcgactgg ttcggtggtc gaacctatgg tacctggctg gcgaacgtgc
      121 ttgatcggct gcgcacgcgc aaggtatttg ccgattggct gtgcatcccg ggaaaacgcg
      181 gtgaacccag caatcgcgtg gttgaagaga cctacccgga tctgatgaca ccatcgcgtg
      241 tcaccctcgt gaagaacgaa gaccgaagat ggatcgatga aggcgcgcat gggtggatcg
      301 aggaatacga ggtgcgcaca acctgagggt gtcagcgaag agggctcact ggtggtcgct
      361 ggcgagtgcc ttaacgacct gcgcgggagt aatctgctcg ttttctgcca gcgtgccttc
      421 gatgaagatc cgatcttgcc tgacgatcag ccgaatgttc ctgaggtgat aggccgtatc
      481 cttggttgtt ttcgtcgtga cataggctgc agcgctttcc ggagtcttga acaccgcgtc
      541 gaagtaggca aagtacccgt cgccaaactg cgtctcgtag cgatcttccc gtaacacgac
      601 cacaagggag ttctcgcttt caaggtttgc ctgtcccatg tgaatctcgg gtcctattct
      661 ttcaagaaac gttctgccag gctcttcggc gcaacaacgt tcttgagatc aggccaggga
      721 atgaccagtt cggtggttcc aagtgcgtag ggcgcgatct cgtaggcgtt gtagcagaac
      781 gtcagtccgc ttttcgagac caggaaattg ttgttcagag cgaagcggcc cttgtcgaac
      841 tgaaagccgg cttgctcata cgtttcgtca ggtttcaggt ttcggacctg gcgaaaaatc
      901 ttctcggcaa tgcggttgag ttcatcgatc ttgtcgacgg cgatcagagc gctgagttcg
      961 attggcttgc cggttttcag ggaatagacc tggtaggtga tttccgaatt ggggtgagcg
     1021 cctcccgtga atatggaggt gatcgtctcc agagcgagca ggtcggcatc agcatgacga
     1081 atggtcgtga cacacttgag cgaccaggct cccggcattt cgggtgtttc cttgaccgag
     1141 gcggcgtatc ctgccgcgaa acgctcgatc agttcatcga tcgtctttgc cggctcccca
     1201 tccgcgaggg ccagaaggcg tgtctgaatc gcactgttga tgcccgtaac cgcagtgtcg
     1261 gccttcaggg acgatgacag ttcggggtat tgaatctcga tctcggccgg gataacatcc
     1321 gggttcttgg tgggcggt
//
LOCUS       DAORHD010001496         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_140496_length_1338_cov_2.332034, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001496 DAORHD010000000
VERSION     DAORHD010001496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140496_length_1338_cov_2.332034"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1338)
                     /locus_tag="PKO06_22935"
     CDS             complement(<1..>1338)
                     /locus_tag="PKO06_22935"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNV72583.1"
                     /translation="GYRHIQQPALSLEKGLRWLSLILETKSFGEPFKPDGKVIITTLD
                     AVPVSHRAICYFPGLSHQAMPGRQQPDPVLADHVRHLLPPLQTAERMRLERLGALAVC
                     WAELPVSAQVCLSLSLSDRSGAALSPSGPFMKVLSEVHGNSVEFARLLNTDVLPMVGS
                     LSETASEPLDVREWLWQASKNGVDTEHLRSLFDARFPLYKRFHVRQSERQQGTTPIHS
                     GLPQWQAAEWDYRDNPNRALSASGISELSGCPFAVFLSRVLRCMPYEQLTTVPLSETG
                     WLDAMQRGNVLHEVYETFLRRAGWPIISQHETLLKQVLEETTEKYRALIPPPSPVIFQ
                     SVKADLLRDVLAFFKLECAAAASGRKPIGFEVSFGMSLPEGKADDLGLNTAEPVELPL
                     PDGRKVYLKGRIDRIDEGPNGLEVWDYKTGSATSYGSGGERAGDARVQMALYQY"
BASE COUNT          285 a          422 c          377 g          254 t
ORIGIN      
        1 atactgatac aaggccatct gaactctcgc atcccccgcg cgttcgcccc ctgagccgta
       61 tgatgtcgcg cttcccgtct tgtagtccca gacctcgagc ccattcgggc cttcatcgat
      121 gcggtcgata cgccccttca ggtagacctt ccgcccatcg ggaaggggca actcgaccgg
      181 ctctgccgtg ttcaagccca gatcatcagc cttgccttcg ggcagactca tgccgaagct
      241 cacttcaaaa ccgatcggct ttcggccgga agcggcggcc gcacattcca gcttgaagaa
      301 cgccagcaca tcgcgaagca ggtccgcttt aacgctctgg aagatcaccg gtgacggggg
      361 cggaatcaaa gctcgatact tttcggtcgt ctcctcgagc acctgtttca gcagggtctc
      421 atgctgagat atgatcggcc acccggctct gcgcaaaaac gtctcgtaca cttcatgaag
      481 cacatttccc ctctgcatgg cgtcgagcca tccggtttcc gacaacggaa ccgtcgtcaa
      541 ctgctcgtaa ggcatacagc gaagaacacg gctcagaaac accgcgaacg gacacccgga
      601 aagttcgctg attccgcttg ccgagagagc tcggttcggg ttgtcccggt aatcccactc
      661 tgcggcttgc cattgcggca gaccagaatg tatcggtgtc gttccctgct gtcgctcgga
      721 ctgacgcacg tgaaaacgtt tgtacaacgg gaagcgcgca tcgaagagcg accgcaggtg
      781 ttcggtatcg acgccgttct ttgatgcctg ccacagccac tctcggacat ccagcggttc
      841 cgaagcggtc tcagagagcg aacccaccat cggcaggacg tccgtgttca acagacgagc
      901 aaactcgaca ctgtttccgt gaacttctga caacaccttc atgaaggggc ccgaagggct
      961 caaagcggcc ccggaccgat cagacaggct cagagagagg cagacctgcg ctgaaacggg
     1021 tagttcagcc cagcagacgg ccagggcacc caagcgctca agccgcatgc gttcagccgt
     1081 ctgcagaggc ggcagaaggt gccgcacgtg gtcggccagc accgggtcgg gctgttgtcg
     1141 gcccggcatc gcctgatgcg acaggccggg gaagtaacaa atcgcgcgat ggctgaccgg
     1201 cacggcatca agcgtcgtga tgatcacctt gccgtcaggc ttgaaaggct caccgaacga
     1261 tttcgtctcg agtatcagac tcagccatcg caggcccttt tcgagactga gcgccggctg
     1321 ttgaatgtga cgatagcc
//
LOCUS       DAORHD010001497         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_140749_length_1336_cov_4.736924, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001497 DAORHD010000000
VERSION     DAORHD010001497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140749_length_1336_cov_4.736924"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PKO06_22940"
     CDS             complement(<1..819)
                     /locus_tag="PKO06_22940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNV72584.1"
                     /translation="MQTLIESFLAYAEKERGFSPHTLRAYRTDLEQFLCWFRQRVATP
                     DTSSLARLHAADFRAYWAMMKTRGMAGTSLRRALAALRACFRFGLRRQMLSRNPLEAA
                     ETPKGHRPLPQILSENELSELLNAPDADDLGLRDRAILELLYGSGLRVSELVSLTLAD
                     LDFAGNTVRVMGKGSKERIVPLTADSIRRLRAYLELPRSEQAGTHLFLNCFGTPLTTR
                     GVARILDKHIRRMALSRQVSPHSLRHSFATHLLNNGADLRAVQEMLGHANIASTQ"
     gene            complement(955..1173)
                     /locus_tag="PKO06_22945"
     CDS             complement(955..1173)
                     /locus_tag="PKO06_22945"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72585.1"
                     /translation="MGSRNRLNYTVIGDGVNLASRLESETKSLNRPIVVSESTRKKAG
                     SDFQFEDLGEVTVKGKTEAVRVYALSRS"
BASE COUNT          294 a          367 c          414 g          261 t
ORIGIN      
        1 ctgcgtcgag gcgatgttgg cgtgccccag catttcctgc acggcgcgca ggtcagcgcc
       61 attgttcagc agatgcgtgg caaaagaatg ccgcagagaa tgtgggctca cctgacggga
      121 aagtgccatg cggcgaatgt gcttgtcgag gatgcgcgcc acaccgcgcg tggtcagcgg
      181 cgtgccgaaa cagttcaaaa acaggtgggt gcccgcctgt tctgaacggg gcagttcgag
      241 atacgcgcgc agacgccgaa tcgagtcggc agtcagcgga acgatacgct ctttcgaccc
      301 tttccccatg actcgcaccg tgttaccggc gaaatcgagg tcggccaacg tgagactgac
      361 gagttcagag actcgtaacc cgctaccata gagcagttcg agaatggcgc ggtcgcgcaa
      421 tccaagatcg tcggcatcag gagcgttgag cagctcgctc agttcgtttt cgctgagaat
      481 ctgcgggagt gggcggtgcc ctttaggagt ttcggccgcc tcgagcggat tgcgactcag
      541 catctggcgg cgaaggccga aacgaaagca ggcgcgcaag gcggcgaggg cacgccgtaa
      601 acttgtaccg gccatgccgc gggttttcat catggcccag taggcgcgga aatcggcggc
      661 atggagacgg gcgagagagg aggtgtccgg ggtggccacc cgctggcgaa accagcacag
      721 gaactgctcg agatcggtgc gataggcgcg cagcgtgtgc ggcgaaaagc cgcgttcttt
      781 ctcggcatag gccaggaagc tctcaatcag cgtctgcaag gtgccgtgct ctcaactgca
      841 agggggtagg ggaagatggc agaagaatag cacaacccta cacacgcgac aagtgggggc
      901 accgaaaaaa tgcaaacttt ttttacagac gttctggttg gcctaaaacg cttttcagga
      961 gcgcgaaagc gcgtagacgc gcacggcttc ggttttgcct tttaccgtca cctcgcccag
     1021 gtcttcgaat tgaaaatcgg accccgcctt ttttcgggtg ctttcactga cgacgatcgg
     1081 gcgattcaga cttttggtct cgctctccag tcgcgaagcc aggttcacgc catccccgat
     1141 gacggtgtaa ttgagtcggt tgcgcgaacc catgttgccg gccacttgct gggatcaggt
     1201 gaaagggcgt caaggctgga ataaacatca aaccaatacg ttccgtcgct aatggttacc
     1261 ccgtatcctg cacaccatcc cggataatca ttcattttgg gaagtaaagc actgcccgat
     1321 gtcagaacgt caacgt
//
LOCUS       DAORHD010001498         1336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_140778_length_1336_cov_3.312256, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001498 DAORHD010000000
VERSION     DAORHD010001498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140778_length_1336_cov_3.312256"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PKO06_22950"
     CDS             complement(<1..208)
                     /locus_tag="PKO06_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72586.1"
                     /translation="MFKHVRDRAIGLACLLYLVICFISPVSLSAAPGNAARELQAAFI
                     DVAKTMKQSVVNIRVEKTQSSPTIR"
     gene            286..420
                     /locus_tag="PKO06_22955"
     CDS             286..420
                     /locus_tag="PKO06_22955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72587.1"
                     /translation="MGGFREIAEAFSQRPEELKEQLQQMVSRGILRRHQGVVEEYIIV
                     "
     gene            complement(434..>1336)
                     /locus_tag="PKO06_22960"
     CDS             complement(434..>1336)
                     /locus_tag="PKO06_22960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV72588.1"
                     /translation="RDEVSVKRERVRLKHELGRLDKEAKSRQHEARRADSRKSKRHLD
                     RHDSDGRGRIGLAIVTGKDGVAGKLASQMQARIQRCQTDLATLQVTPEYQLQFRLPGS
                     ISSRNHLLHLEAGQIALGPERTLSFPRLIMGTRDRIALVGPNGSGKSLLLSHLIPHLN
                     VDHERLVYLPQEISLDRSRAVMQELRAQPRQQLGTIFSIISCLGSRPERLLESEQASP
                     GEIRKVLLALGIAREPHLIIMDEPTNHLDLNAIELLEQALQGCPSAMLLISHDRRFLQ
                     NLTVSSWRIVSTGTDQQLVFDNEV"
BASE COUNT          277 a          351 c          407 g          301 t
ORIGIN      
        1 agcgaatcgt tggggaagac tgggtcttct cgacgcgaat gttgaccacg ctttgtttca
       61 tcgttttcgc gacatcgatg aacgcagcct gcagttcgcg ggcggcgttt ccgggtgcag
      121 cagagagtga tacgggcgat atgaaacaga tcaccagata cagaagacag gccaggccga
      181 tggcccggtc acgcacatgt ttgaacatac tcagcctcct cgggttgttt tcgttgagat
      241 gatcgtattg accagcgact cgacgcagag gttcccggaa tttgtatggg aggttttcgt
      301 gagatcgcag aggctttttc acagcggcct gaagaactga aagaacagct gcagcagatg
      361 gtctcccgtg gcattctgcg caggcaccag ggagtggtcg aagaatacat tatcgtgtaa
      421 gagggctcag acctcagacc tcattgtcga acaccaattg ctgatcagtc ccggttgata
      481 cgatgcgcca ggagctcaca gtcaggtttt gcagaaatcg acggtcgtgg ctgatcagaa
      541 gcatcgcgct tggacaaccc tgcagagcct gctcgagaag ctcgatggcg ttcagatcga
      601 gatgattggt gggttcatcc atgatgatca aatgaggttc gcgggcgatt cccagagcca
      661 gcagcacttt tcgaatctcc ccgggacttg cttgctcgct ttcgagaagc cgctctggcc
      721 gcgaaccgag acaactgatg atcgagaaga tggtgccgag ctgctgtcgg ggttgcgctc
      781 gcagctcctg catgaccgca cgtgatctgt ccaaactgat ctcttgcgga agatagacca
      841 ggcgctcgtg atcgacattc agatggggaa tcagatgcga gagcagcaga ctctttccag
      901 acccattggg tccgacgagg gcgatacggt cacgcgttcc catgatcagc cgggggaagg
      961 agagggtgcg ttccggcccg agggcgatct gcccggcttc gagatgcagc aggtgattcc
     1021 gcgacgatat agagccggga agacgaaatt gcagctggta ctccggggtg acctgaagag
     1081 tggccagatc ggtctggcac cgctgaattc gagcctgcat ctgactggcg agtttgccgg
     1141 cgacaccgtc ttttccggtg acaatggcca ggccgatccg tccgcgaccg tcagagtcgt
     1201 gtcgatccag atgccgtttg ctttttcgcg agtcggcgcg acgagcctcg tgttgcctcg
     1261 attttgcttc tttgtccagt cgtccgagct cgtgtttcag tcgcacgcgt tcgcgtttta
     1321 cactcacttc gtcgcg
//
LOCUS       DAORHD010001499         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_141478_length_1332_cov_3.855912, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001499 DAORHD010000000
VERSION     DAORHD010001499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141478_length_1332_cov_3.855912"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1222
                     /locus_tag="PKO06_22965"
                     /pseudo
     CDS             311..1222
                     /locus_tag="PKO06_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942713.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
BASE COUNT          313 a          350 c          385 g          284 t
ORIGIN      
        1 gcggacggcg tgcacgttgt tgatattcat aaacttgcag tagtagaacg ctttttcacg
       61 gggaacggat ctgatgcgat ggccatctgg tacccgccgc cttgaaggcc ggcatgactc
      121 agttgacgcg ttcccgtctt ttccagaaat ttgagtgatc actttttcat tctgttgagt
      181 tcccacgctc agcccgcttt cacacgttct tcgcaccatg cgatcctgcc gcctgacccc
      241 cgagcatgcg cagtacgttc gggggttttt gaggtgccag gaatgcacag aaacctgagg
      301 agctgccagt atgcccagac gcgacgacat caaaaaggtc atgattatcg gttccggtcc
      361 catcatcatc gggcaggctt gtgagtttga ttattcggga acacaggcgt gcaaagctct
      421 caggaggctt ggataccaga tcgtccttgc aaactcgaat ccggcgacaa tcatgacgga
      481 cccgggaatg gcagatttca cctacatcga acccttgaac aaagaaacga tggaggagat
      541 catacgcaag gaacggcccg acgctcttct gccgaatctc ggagggcaat ctggtctgaa
      601 cctttcctct gaactggcag cagcgggtgt tcttgacaaa tacggcgtga aggtcatcgg
      661 ggtgaatgtc gatgcgatca aacgcgggga ggatcgtctc gcgttcaaac agaccatgga
      721 aagcctcgga atcgacatgc cgaaaagtga gatcgccaac agtgtcgaag aggctgaaga
      781 gatcgcgcag cgcctcgatt accctctggt ggtgaggccg gcgtatacca tgggagggac
      841 tggcggaggt tttgtgtaca acctcgaaga gctcaggact atcgtcgccc gcggcctggc
      901 cgcaagtctc gtcgggcagg tgctgataga gcagtcggtg ctcgggtggg aggaactgga
      961 gctggaagtc gtccgcgacg ccaaaaacca gatgattacc gtgtgcttca tcgaaaacgt
     1021 ggatgcgatg ggtgtacaca ccggagattc gttctgcact gccccgatgc tcaccatctc
     1081 ccccgaactc cagcgcaagt tgcaggatta ctcgtatcga attgtcgagg cgatcgaggt
     1141 tatcggtgga acaaacatcc agttcgcgca cgaccccaaa acaggtcgcg tcgtcgtgat
     1201 agagatcaat ccccggacct ccggagaatt caggaaaacg gtgacgggaa accttgtgag
     1261 gaaatttctc ctgtccctcc tccagaactg acggtcgggg gaaagtcccc catgttgatt
     1321 tgggcggaaa ag
//
LOCUS       DAORHD010001500         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_141587_length_1332_cov_2.306969, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001500 DAORHD010000000
VERSION     DAORHD010001500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141587_length_1332_cov_2.306969"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..600)
                     /locus_tag="PKO06_22970"
     CDS             complement(148..600)
                     /locus_tag="PKO06_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72589.1"
                     /translation="MKKIWLIAGMTIFMAHGSLASAEEVKARDISGRIKELRERLSQF
                     QARGEEPKTAGGAVKTQEQDPEKLTDQLEHRQDAKMLVLFHDQGTKAEASGIQNPTLD
                     DERETNSDSVNEAEPSAVEKALQRLRESRLARQTRLAKADLPGTGEKK"
     gene            780..>1332
                     /locus_tag="PKO06_22975"
     CDS             780..>1332
                     /locus_tag="PKO06_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72590.1"
                     /translation="MTLATDRINQNHGAASFTHLMKSGSSDKEASGDFAALLMNLMDQ
                     RPAGAETLSFPARTSLSSFQKRDDFFSRPPSLMPRRSTAETPRATPMVKTEARQEPEA
                     TSAKSNDETEKTASQDTESVAQNSDTVTEADASEVKPVADSTPISMEEEAASLVSMVP
                     VIVNDLLKSLEDDEQVQSLLAGLQ"
BASE COUNT          298 a          400 c          334 g          300 t
ORIGIN      
        1 ccaacgttta ctctcgctga gagactggtt tcgctgtttt ctttcgtcac gcacccatcg
       61 ccagccacga ccactcgatc tccccctcaa agcaaaaggg ccgcgagtcg tttctctgac
      121 ccgcggccct cttcctactc agaaaagtta ctttttctcg ccggttcccg gaagatccgc
      181 tttcgccaat cgcgtctgac gggccagtcg cgactctcgc aggcgctgca acgctttttc
      241 gacggcagag ggttcggctt cgttcacgga atcactgttg gtctcgcgct catcatcaag
      301 agtggggttt tgtattccag aagcttccgc tttcgtcccc tgatcatgga acagaaccag
      361 catcttggca tcctgacggt gttcaagctg atcggtcaat ttttccgggt cctgttcctg
      421 agttttgacc gctccgccag ccgtcttcgg ttcttcgcct cgggcctgaa actgcgacaa
      481 ccgttcgcgt aattccttga tgcgcccgga gatatcccgc gccttcacct cttcagcaga
      541 agccaggctt ccgtgagcca tgaaaatcgt cattccggca atcagccaga tttttttcat
      601 cgtcgttccc tccgtgaacc agtgttcctg agatctatcg gcaatccgac gacgggtcac
      661 cacctcgacg aatcagaaat gttgcgtcga atggaaaaaa agtattcagg ttcaggcgag
      721 aaatgacgat gaagatagtg ggaaagtcta aattgacggc cccgagccag gaggcagaca
      781 tgacattggc aaccgaccgc atcaaccaga accacggtgc ggcatcattc actcatctga
      841 tgaagtccgg aagctccgac aaagaagctt ctggtgattt cgccgctctc ctcatgaatc
      901 tgatggatca gcgaccggca ggtgcggaaa ctctttcctt tcccgcacga acgtcgttat
      961 cttcgttcca gaaacgagat gatttctttt cccgcccccc ctctttgatg ccacggcgct
     1021 caacggctga aaccccgaga gcaactccaa tggtgaagac cgaggcgaga caggagccag
     1081 aagcgacctc tgccaagagc aacgacgaga ccgagaaaac ggcctcgcag gacaccgagt
     1141 ctgtcgcgca gaattccgac acggtcacag aagccgacgc ctcagaagta aaaccggtgg
     1201 cggattcgac acccatctcg atggaagaag aggcggcctc tctcgtctcc atggtgcccg
     1261 ttatcgtcaa tgatctattg aaatcgctcg aggacgatga gcaggtgcag agcctgttgg
     1321 ctggcctgca ac
//
LOCUS       DAORHD010001501         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_141853_length_1330_cov_4.247059, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001501 DAORHD010000000
VERSION     DAORHD010001501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141853_length_1330_cov_4.247059"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1330
                     /locus_tag="PKO06_22980"
     CDS             <1..>1330
                     /locus_tag="PKO06_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72591.1"
                     /translation="NLLPKANITLQVPETPWAIPYTGTTAELATLTIVTTPTTPTGLY
                     TGEIIFWEDSTVNGSRDADEDAWALKVTLAVGVKDLEIIEPALDFGVVAKGSNSTTSS
                     MTIRNKGTVFLSNTRVIPGSLTGGAGTIPPSDLHFGTLQVTPPNLNPGVTRAVSLYIT
                     AAAGLPSGIYTGAQRVFEDLNGNGAIDASEPVDLATVTVMVTSGGALGFEFFNNPGTV
                     DFGIVARSGNATASFQIISLSGSTLSNLQYQKADLMAGPTPLSQANLTFSPAPGYSIP
                     PFATSDATATVTIPPLQLAGTYIGQQWVVDADHLPGASAPLILTVTVPPASLSVSPTT
                     VDFGYQAPGANASTTFTIQNLSANALDIDIVKSPLLRNGVGPESIPITDLSFAPQLWN
                     LQGPPAVPNSTLATLGVMIDATTLPGTYLGTCTFRDLTFPGESQAQLSVLL"
BASE COUNT          269 a          449 c          349 g          263 t
ORIGIN      
        1 gaacctcctg cccaaagcca acatcaccct gcaggtgccc gagaccccct gggccattcc
       61 gtataccgga accaccgccg aactggcgac cctgaccatt gtcaccaccc cgacgacgcc
      121 gacgggcctg tataccggcg agatcatctt ctgggaagat tcaaccgtga acggcagtcg
      181 cgatgcggat gaagatgcct gggcgctgaa ggttaccctc gcggtaggcg tgaaggacct
      241 ggaaatcatc gaacccgccc tggactttgg cgttgtcgcc aaggggagca acagtacgac
      301 ctcgtcgatg actattcgca acaagggcac cgtctttctt tccaatacac gcgtcatacc
      361 aggctctctc accggtggag ctggtactat tccgccatca gaccttcact tcggcacttt
      421 gcaggtgaca ccgccgaatc tgaatccggg cgtcacgcgc gcggtcagcc tgtatatcac
      481 cgctgctgcc ggtctcccgt ctggcatcta caccggagcg caacgggttt tcgaagacct
      541 gaacgggaac ggcgctatcg atgccagcga acccgtggac ctggcaaccg tcaccgtcat
      601 ggtaacttcg ggaggcgcac tgggctttga gttcttcaac aatcccggaa ccgtcgactt
      661 cggtatcgtc gccagaagcg gaaacgcgac agcttcgttc cagatcatca gtctgagtgg
      721 cagcacgctc agcaatctgc agtatcagaa agcggatctg atggccggtc caacaccgct
      781 gtcgcaagcg aatcttacct tctctccggc gcccgggtac tcgattcctc cgttcgccac
      841 ctcggacgca actgcgaccg tcacaatccc accgctgcaa ctggccggca cgtacatcgg
      901 ccaacagtgg gtggttgatg ccgatcatct tccgggagct tccgctccgc tcattctcac
      961 cgtgacggtt ccaccggcca gtctgagcgt cagccccacg acggtcgatt tcggctatca
     1021 agctccgggc gcgaacgcca gtacaacctt caccattcag aacctgagtg ccaacgcact
     1081 cgacatcgac atcgtcaagt ctccgctgtt gcgcaacggt gtggggccgg aatccattcc
     1141 gatcaccgac ctctccttcg ccccccagct ctggaatctc caggggccgc ctgctgtccc
     1201 gaacagcacg ctcgcaactc ttggagtgat gattgacgcc acgactctgc cgggaacgta
     1261 tctcggcacg tgtacctttc gcgatctgac cttcccggga gaaagtcagg cccaactctc
     1321 ggtgcttctg
//
LOCUS       DAORHD010001502         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_141860_length_1330_cov_3.613333, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001502 DAORHD010000000
VERSION     DAORHD010001502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141860_length_1330_cov_3.613333"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..582)
                     /locus_tag="PKO06_22985"
     CDS             complement(139..582)
                     /locus_tag="PKO06_22985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV72592.1"
                     /translation="MAANLNRVFLVGNLTRDPELNYTPQGTAVGKFSIAVNRQYKGSD
                     GEMKKQTDFFNIVVWGKTGETCKKFLTKGRSVLVEGRLQNRSYETQDKQKRTVTEIVA
                     DNVQFLGGSMGGGSRPDDGGYAGGGEPAADETFVDTPPGNDDVPF"
     gene            complement(596..889)
                     /gene="rpsF"
                     /locus_tag="PKO06_22990"
     CDS             complement(596..889)
                     /gene="rpsF"
                     /locus_tag="PKO06_22990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013789108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNV72593.1"
                     /translation="MTVKAYETLFFTEPEISEEALTTLLTKVEGAITRYGGEIEKVNK
                     WGRRPLAYRVDKFNEGYYILVDFKGNPEVLKELERFYLINQNILRFLTTSRLQ"
     gene            1075..>1330
                     /locus_tag="PKO06_22995"
     CDS             1075..>1330
                     /locus_tag="PKO06_22995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72594.1"
                     /translation="MPTFLHSIASRGGILRLFLVIALTPLLLATIGMAATPDDSRVKP
                     HPATRAAAENSAAHGSPSAHIGAPRWDLTSFFPSFSDPSRL"
BASE COUNT          279 a          390 c          345 g          316 t
ORIGIN      
        1 tgcagaaatg gcagatcttg cgatgccgcg ggcggcgcat ttcgtgaccg ccggaagacg
       61 ggccggagga catgggagcg gaagaagggg catgagtgtt ttcagccatg attgaatatc
      121 tccttgaatt tcaggtgatc agaagggaac gtcgtcgttc cctggggggg tgtcgacaaa
      181 agtctcatca gctgcgggtt cgccgccacc ggcatacccg ccgtcatccg gacgactgcc
      241 accgcccatc gagccaccca gaaactgcac gttgtcggcg acgatttccg ttacagtgcg
      301 tttctgcttg tcctgtgtct cgtaggaacg gttctgcaac cgaccttcga ccaaaaccga
      361 ccgacccttg gtgaggaact tcttgcaggt ctcgccggtc ttgccccaga ccacaatatt
      421 gaagaagtcg gtctgttttt tcatttcccc gtcagaacct ttgtactgac ggttcacagc
      481 gatcgagaac ttgccgacgg ccgttccctg cggagtatag ttcaactccg ggtcacgggt
      541 cagattcccg accagaaaga cgcggttgag gttcgctgcc ataatgccgg tgcctctact
      601 gcaagcggct ggtggtcagg aagcgaagaa tgttctggtt gatcagatag aaacgctcga
      661 gttctttcaa aacctcgggg ttccctttga aatcgaccag gatgtagtat ccttcgttga
      721 acttgtcgac ccggtaagcc aacgggcggc ggccccactt gttgactttc tcaatctcgc
      781 caccgtagcg ggtgatcgcg ccttccacct tcgtgagaag agtggtgaga gcctcttcag
      841 aaatttcggg ctcggtaaaa aataaggtct cgtatgcctt cacggtcata cctccctgtg
      901 gattaatagc cttcggataa acccgaaagc aggggacgtg atgggtaagt gtaacacgat
      961 gtacaacctg atgcaacttc cagattcgct tctcttctac gtattcctct ttcgttcctc
     1021 tacctgtcgg gtatatagta acgtctcgtg aaccactcca ccaaggataa aaccatgcct
     1081 actttccttc attctatcgc cagtcgaggg ggtatccttc gcctctttct ggtcatcgct
     1141 cttacacccc tgctgctggc aacgatcggc atggccgcaa cacctgatga ctccagagta
     1201 aagccacacc ccgccacccg cgccgcggcg gaaaacagcg ccgcgcacgg ttcaccatcc
     1261 gctcacatcg gagcgccgcg atgggatctc accagcttct tcccgtcttt ttccgatcct
     1321 tcacggctgc
//
LOCUS       DAORHD010001503         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142245_length_1328_cov_3.662215, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001503 DAORHD010000000
VERSION     DAORHD010001503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142245_length_1328_cov_3.662215"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..682
                     /locus_tag="PKO06_23000"
     CDS             350..682
                     /locus_tag="PKO06_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72595.1"
                     /translation="MQKNRKQGSAILGLLMGVIMIALLSTMYFSGSGGTSAPQAIQHI
                     DDGKAAACGMIRQTLETQVQLWQVQNPGQKPTIENLTGSTIGTAKCPGNGSYTFGADN
                     RIHCNKHK"
     gene            733..>1328
                     /locus_tag="PKO06_23005"
     CDS             733..>1328
                     /locus_tag="PKO06_23005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV72596.1"
                     /translation="MSTETTPSYRGYWALIISQFQGAFNDHAFKNLLLLVALSLDPSV
                     EHSNKWSSLIPAVFIAPFLLFAMHSGFLADRYSKRTIIIGTKWLEIAIMLGAGLAFLS
                     GHLYFGLLMLFLAGTQAAYYGPSKYGILPELVREKHLSWANGIIELTTFAAIILGTFA
                     GTLFFVHFKASLHYAALVLTGLSVLGLAASYRIDEVQAA"
BASE COUNT          286 a          418 c          343 g          281 t
ORIGIN      
        1 ctgcggatca agcccgccaa cctgcgctga gggccagacc gaccaccagg gccacggacc
       61 agatcgtcaa gcgtttcatt cctctaacct ccagcaatat tgactatgga ctgagcttat
      121 caccattcag gtcaccacgg caagaataag accacccggt tcactcatcg gcacggcaac
      181 agagcccccc acccctgccg cttcttatgt tacatgaagt atgcaaacga aacaaaacgc
      241 cctcgctctt gatcacccag cttcgaaacg ttcgagaaag ttggtatgcc gcgagttgta
      301 cgctaccatg ctgtcgcatc gtgacgattt tcagaggggg aggttcggca tgcagaagaa
      361 caggaaacag ggatcggcca ttctcggtct gttgatgggc gtcatcatga tcgctctctt
      421 gagtaccatg tatttttccg gttcaggtgg caccagcgcg cctcaggcca ttcagcatat
      481 tgatgacggc aaggcggctg cctgcggcat gattcgccag acactcgaaa cgcaagtgca
      541 gctgtggcag gtgcagaacc ccggtcagaa gccaaccatc gagaacctga ccggcagcac
      601 gatcggcacc gcgaagtgcc ccgggaacgg gagctatacc ttcggcgccg acaaccgcat
      661 tcactgcaac aagcacaagt aagtcacccc tgatcgacac tcgataaccg ccagacaacg
      721 agaggaacac gcatgagcac tgagaccacc ccttcctatc gaggttactg ggccctgatc
      781 atctcgcagt ttcagggggc cttcaacgat cacgcgttca agaatctgct gcttctggtc
      841 gccttgagcc ttgacccgag tgtcgagcac tccaacaagt ggagctcgct gattcctgcg
      901 gtcttcatcg cgccgtttct gctgttcgcg atgcacagcg gttttctggc tgaccgttac
      961 agcaaacgca ccatcatcat cggtaccaaa tggctcgaga tcgcgatcat gctcggcgcc
     1021 ggcctggcct ttctctccgg gcacctgtac ttcggtctgc tgatgctgtt tctggcgggc
     1081 actcaggcag cctattacgg gccttccaag tacggcattc ttcccgagct ggttcgcgag
     1141 aagcatctgt cgtgggcaaa cggcatcatc gagctgacca cgttcgccgc gattattctg
     1201 ggaacctttg ccggaaccct gttcttcgtc catttcaaag cctcactgca ctacgcggcc
     1261 ctggtactga ccggcctgtc ggtcctgggt ctcgccgcct cctaccgtat cgacgaggtc
     1321 caagccgc
//
LOCUS       DAORHD010001504         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142252_length_1328_cov_3.449332, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001504 DAORHD010000000
VERSION     DAORHD010001504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142252_length_1328_cov_3.449332"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PKO06_23010"
     CDS             <1..1142
                     /locus_tag="PKO06_23010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV72597.1"
                     /translation="KVLAGAQETAMGGITQTKEIAGGAVSSLNMASQVVTWGLLGAVV
                     LGIFIAVTITRSITGPLNTAVNQMSAGAEQVAAASAQISSSSQQLAGGASEQASSLEE
                     SSAALEELAAQAKNNFEKAREATDGAEKAKEAAEKANVAMGQTVQSMNDIKESSGKIS
                     GIIKTIEEIAFQTNLLALNAAVEAARAGEHGKGFAVVAEEVRNLAQRSAVAAKDTAAL
                     IENSVQQANRGADVVKSASDAINQILGVVAGVADNAKQVTTASKEQSEGITQINTAVA
                     EMDKVTQGVAANAEESAAASEELNAQATTMKEIVNSLVALIHGGTGDGQMTADHPAGM
                     PTARRVPHIDLESKPSDFAKKAGKKPAKPAPGGQKPPAPGDVGDF"
BASE COUNT          300 a          382 c          433 g          213 t
ORIGIN      
        1 ggaaggtact ggctggcgcg caggaaactg ccatgggtgg cattacccag accaaggaaa
       61 tcgcgggcgg ggccgtatcg tctttgaaca tggcctctca ggtcgtgacc tggggtctgc
      121 tgggcgccgt cgtgctcggc atcttcatcg ccgtgaccat cacccgctcg atcaccgggc
      181 ctttgaacac cgcggtcaac cagatgagcg ctggggctga acaggtcgcc gccgcctcgg
      241 ctcagatctc gtcgtcgagc cagcagctgg cgggcggagc ttcggaacag gcgtcgagcc
      301 tcgaagaatc gtcggcagcc cttgaagagc tggcggcgca ggcgaagaac aacttcgaaa
      361 aggcccgcga agcgacggat ggcgcggaaa aggccaagga agcggccgaa aaagccaacg
      421 tggccatggg ccagaccgtg cagagcatga atgacatcaa ggaatcgtcg ggcaagatct
      481 cgggcatcat caagaccatc gaagagatcg ccttccagac caacctgctc gccctgaacg
      541 ccgccgtcga ggccgctcgc gcgggtgaac acggcaaggg cttcgcggtt gtggccgaag
      601 aagtgcgcaa tctggcccag cgctcagcgg tcgccgccaa ggataccgcg gcgctgatcg
      661 agaacagcgt gcagcaggcc aatcgtggcg ccgatgtcgt gaagagcgct tctgatgcga
      721 tcaatcagat tctcggcgtg gtggccgggg tcgccgacaa tgccaagcag gtgacgacgg
      781 cctcgaagga acagtccgaa ggcatcacgc agattaacac tgccgtcgcc gagatggata
      841 aagtgactca gggcgtcgcc gccaacgccg aagagtctgc cgcggccagc gaagagctga
      901 atgcacaggc gacgacgatg aaagagattg tgaactctct ggtggccttg attcatggcg
      961 ggaccggcga tgggcagatg accgctgatc accctgccgg catgccgacc gcgcgtcgag
     1021 tcccccacat tgatctcgag tccaagccgt cggattttgc gaagaaagcc ggaaagaaac
     1081 ccgccaagcc tgcgcccggt gggcagaaac cccccgcgcc cggcgacgtc ggtgacttct
     1141 aagaactgaa gtgttgctct cacccgcttc ttacgaggcg ggtgagagtc ttcaggggtg
     1201 aatcagaacc tttcgatttc gtcgaacaca tggtatgata gtgcctgcct gtccgagttt
     1261 gctcgttcag gaagagtgat caacaaagga aatagccatg agcgagagga aggggacgga
     1321 ggaagtcc
//
LOCUS       DAORHD010001505         1328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142271_length_1328_cov_3.112333, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001505 DAORHD010000000
VERSION     DAORHD010001505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142271_length_1328_cov_3.112333"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..638)
                     /locus_tag="PKO06_23015"
     CDS             complement(144..638)
                     /locus_tag="PKO06_23015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV72598.1"
                     /translation="MRRGFSMVEILVALMFVSFAFLPIYNLFRFGHHGTVSNEREIEA
                     TNYASDIINFLRTQKTQIIDQKIPEAATAEYALTDKDFFNRFQLMAEGFSMSTGFTRK
                     IILKKYRGSDKTGPGGFIGARKDWRFNRVSVPNYLVHVHVKFPKGLASNLEDDVFLSS
                     IIMD"
     gene            complement(646..>1328)
                     /locus_tag="PKO06_23020"
     CDS             complement(646..>1328)
                     /locus_tag="PKO06_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72599.1"
                     /translation="DPAPMPDRPLREPLPGGPYEPGFPAIIAGVGGCMVDASHLKAGL
                     RLTLQPGETAQINGQAVYLPEPGQEISIHGRSQGIFTRGALLWHNGDAELSFKLVKPF
                     RVVLGDSFVTIRGTVIHFKGEVQGSVQIQLLSGVAELTTPRGVRLMTVGETVDVRQMP
                     TNPVPRPVSATPTPPIVSPDPSDTTTNPVPVPVGESIPTATGTGVSSVTLQGNPYEDA
                     PVVVPGSQ"
BASE COUNT          308 a          363 c          375 g          282 t
ORIGIN      
        1 tcgcagattg gtgagcttgc tctgattttt gtggaatgat ccccaggtgc cggcgaataa
       61 cttgtacatc gtggagaaaa ccacgatcat cagggcaccc atggccagaa tttcgacgag
      121 agtaaaggcg cgacgcaaca tgatcaatcc atgatgatcg acgacagaaa gacatcgtct
      181 tccagattcg aggcaagacc cttgggaaac ttcacatgaa catggacgag atagttcggc
      241 acggagacgc gattgaacct ccagtccttg cgagcgccga taaatccgcc cggccctgtc
      301 ttgtcgcttc cgcggtattt cttcagaatg atttttcgcg taaaacccgt agacatgctg
      361 aaaccttcgg ccatcaactg aaacctgttg aaaaagtcct tgtcggtaag cgcgtattca
      421 gctgtggctg cctcggggat tttctgatca atgatttgcg tcttctgagt acgcagaaaa
      481 ttgatgatgt cggacgcata attcgtcgcc tcgatttcgc gctcgttgct gacagtcccg
      541 tggtgcccga agcgaaacaa gttgtagatg ggcagaaaag caaaactgac aaacatgagc
      601 gcgaccagaa tctcgaccat ggaaaacccg cgtcgcatgg gcaggttatt gagatccggg
      661 cacaaccacg ggagcgtctt cgtaggggtt cccctgaagg gtgacactcg ataccccggt
      721 gcccgtggca gtcggtatcg actcgccaac cggcaccgga acaggattcg tggttgtatc
      781 ggatgggtcg ggcgacacga ttggtggggt tggcgtcgcg ctaaccggcc gtggcacagg
      841 attcgttggc atctgtcgca cgtcgacggt ctcaccgacg gtcatcagcc gtaccccgcg
      901 aggggtcgtc aactcggcaa ccccgctcaa cagctgtatc tgcacgcttc cctgaacctc
      961 gcccttgaaa tgtatgacgg taccgcgaat ggtgacaaag ctgtctccca gtaccacccg
     1021 aaatggtttc accaatttga aagacagttc ggcatcacca ttgtgccaca aaagagcccc
     1081 gcgtgtgaag ataccctgtg atcggccatg aatcgagatc tcctggccag gttctggcag
     1141 atacacggcc tgaccgttga tctgcgcggt ttcacctggt tgaagcgtca ggcgcaagcc
     1201 ggctttgagg tggctggcat cgaccatgca accgccaacc ccggcaatga tggccggaaa
     1261 ccctggctcg tatggtccgc ccgggagagg ttcacgcaat ggacgatcgg gcatgggcgc
     1321 aggatcag
//
LOCUS       DAORHD010001506         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142468_length_1327_cov_3.287736, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001506 DAORHD010000000
VERSION     DAORHD010001506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142468_length_1327_cov_3.287736"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1327
                     /locus_tag="PKO06_23025"
     CDS             <1..>1327
                     /locus_tag="PKO06_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72600.1"
                     /translation="RGWDTGQYAAGKDLWWNSNNCDSYIQRDGKQPHSGKYSLHLVNR
                     SAKAPHTYGTTQQLLSIVPGQRYRVTLWAKGKQLTSPGSVSIVVDEAWSVRPIVLPQG
                     SFDWTQFTGTFSLGTKQTSLRLLMEGPGEVWLDDLTIEPATDSEPVVNAPVAASEMLN
                     QVDEESDLPADLDLEASGERFDESGQRADLDAGGGAGDVFAEFKRGDSEKPNFDDLPG
                     TGEPGSMDPTDGPGVVSHPGDPDRKPLTVSVSASSARVPASTWVRFVARARGGTKPYT
                     FAWTDDGVAKNSRSANLSLLWKKKSVNVVSVLVTDSSNPPLQARANVTIIVDEPSKEP
                     SRPVLSLQSVILSPASVSPGGQVKVNAIFRLQHWPASAAVIEATFAMETSSRGSVVRK
                     VFRFRCPVKNGQAYSNVWVRFPTRADARPGPIRAAVKLRVSGLVARKMGH"
BASE COUNT          283 a          340 c          416 g          288 t
ORIGIN      
        1 ccgtggctgg gatacggggc agtatgccgc gggcaaagac ctgtggtgga acagcaataa
       61 ctgcgactcg tacattcagc gcgatggaaa acaacctcac agcggaaagt attcgctgca
      121 tttggtgaat cgaagcgcga aagcgccgca tacctatggt acaactcaac agctgctctc
      181 gattgtgccg gggcaacggt atcgagtgac gttgtgggcc aaggggaagc agttgaccag
      241 cccaggttcg gtttccattg ttgtcgatga agcctggagt gtcagaccga tcgttcttcc
      301 tcagggaagc tttgattgga cgcagttcac gggaacgttt tctctcggga cgaagcaaac
      361 ttcccttcgg ctgctgatgg agggcccggg cgaggtgtgg ctggacgatc tcacgattga
      421 gccggcaact gactcagaac ccgtggtgaa cgcccctgtt gctgcttctg agatgctgaa
      481 tcaagtcgac gaagagtcgg atctgccagc ggatctggac ctcgaagctt ccggagagcg
      541 gttcgacgaa tcaggtcaga gagctgatct cgatgcgggc ggaggagccg gcgatgtctt
      601 tgcggagttc aagcgcggtg acagtgagaa gccgaacttt gacgacctgc cgggaacggg
      661 agaacccggt tcgatggatc cgacggatgg tcccggtgtc gtctcgcatc cgggagatcc
      721 cgatcgaaag ccacttacgg tcagtgtctc cgcgagttcc gcgagagtgc ctgcttcaac
      781 ctgggtgaga tttgtcgctc gtgccagagg cggtacgaaa ccatacacgt ttgcctggac
      841 cgacgacggg gttgcgaaaa acagtcgcag cgcgaacctc agtcttctct ggaaaaagaa
      901 aagcgtgaac gttgttagtg tcctggttac agattcatca aatccgccac tgcaggcaag
      961 ggcgaacgtc acgattatcg tcgatgaacc gtcgaaggag ccttccaggc cggtgctgtc
     1021 tctccagtcc gtcattctat ctccagcttc ggtatcccct ggcggtcagg tgaaagtgaa
     1081 cgccattttt cgcttgcagc attggcctgc atctgctgcg gttatcgagg cgacttttgc
     1141 catggaaacc tcaagtcgcg ggtctgttgt tcgcaaagtg ttccgttttc gttgtccggt
     1201 gaagaatgga caggcataca gcaatgtgtg ggtacgattt ccaacgcggg ccgatgccag
     1261 acctgggccg attcgggcgg cggtgaagct gcgtgtgagc ggcctggtcg ccagaaaaat
     1321 gggccat
//
LOCUS       DAORHD010001507         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142591_length_1327_cov_1.873428, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001507 DAORHD010000000
VERSION     DAORHD010001507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142591_length_1327_cov_1.873428"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKO06_23030"
     CDS             complement(<1..815)
                     /locus_tag="PKO06_23030"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV72601.1"
                     /translation="MLDSTTLRNLSIFTGYSHIGWCREFVWIPGDRIGNQKMTSRFIV
                     RGLQCMYDVEALMIRKDLYLRTDEGKEYVVNNFQILDVEDVENIKGIAENCEVLFEKF
                     PEMKSITEGERVVISKQTQVMAFKAVSTVMNSVRMGQGIDGKESKEVVSSMVEDITSN
                     PDAIMNLINIKSFDDYTFTHNINVATISVLIGQQLNLPNADLQELGMGALMHDVGKLK
                     VSLAILNKDGKLTDAEFAEMKRHPKYGYEILCKSNDISERARMVALQHHEKFQG"
     gene            complement(988..>1327)
                     /locus_tag="PKO06_23035"
     CDS             complement(988..>1327)
                     /locus_tag="PKO06_23035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72602.1"
                     /translation="VFLELNFIENPRTIPTGTVILVPTMDEMQKIAKEKDANRRKELM
                     NNIRQGTPGARVDAAGATAEGKKPAVSSNAAAPVPVKIDAKKASFKNVMNAQFTDENA
                     VKHTNVGKDK"
BASE COUNT          296 a          357 c          308 g          366 t
ORIGIN      
        1 ccctgaaatt tttcatggtg ctgcagagcc accatgcgcg ctctctctga aatgtcattc
       61 gatttgcaga ggatctcata cccgtatttt gggtgccgct tcatttcggc gaactccgca
      121 tccgtgagtt tgccgtcttt attgagaatc gccagcgaca ctttcagttt gccaacatcg
      181 tgcatcaggg cacccatgcc taactcctgc aggtctgcat tgggcaaatt cagttgctga
      241 ccaatcagaa cggagatggt ggccacattg atattgtgtg taaacgtgta atcatcgaaa
      301 gatttgatgt tgatcagatt catgatggca tcaggatttg aagtgatatc ctcaaccatc
      361 gaactgacca cttccttgga ttccttgcca tcgataccct gacccatacg cacgctgttc
      421 atgacggtgc tgaccgcctt gaacgccatt acctgcgtct gtttggaaat gacgacgcgc
      481 tccccttccg tgatactctt catttcaggg aacttctcga acagaacctc gcagttctcg
      541 gcgattccct tgatgttctc aacatcttcg acgtcgagaa tctggaagtt attgaccacg
      601 tattccttgc cttcatcggt gcggagatac aagtctttgc ggatcatcag cgcctcgacg
      661 tcatacatgc actgcaaacc gcgcacgatg aaacgtgagg tcattttctg gttgccgatc
      721 cggtcgccgg gaatccagac aaattctcgg caccagccaa tatggctgta accagtaaaa
      781 atcgaaagat tccgcaaggt cgtactatcg agcataattg tgcaccatac taccgcggtt
      841 tttcgaaaaa cacaatggta tgcccgtgag atcaatgatc accgctgtag tttgtcccat
      901 ccctgcgaat gggatcgtcg agaccaaaaa cagcaatcga ggggccgtat cctggcggat
      961 acgacccctc gattgctgtt ctggtcgtta cttgtctttc ccgacgttcg tgtgtttcac
     1021 cgcattttca tcggtaaact gagcattcat gacattttta aatgaggctt ttttcgcatc
     1081 aatcttgacc ggcacaggag cagccgcatt cgaagaaacg gcaggctttt tgccttctgc
     1141 ggtcgcaccg gctgcgtcga cccgcgctcc tggcgtccct tgccgaatgt tgttcatcag
     1201 ctcttttcgg cgattcgcat ctttttcttt ggcaatcttc tgcatttcat ccatggtcgg
     1261 aaccagaatg acggtacccg tcggaatggt ccgcgggttc tcgatgaagt ttagctcgag
     1321 aaacacg
//
LOCUS       DAORHD010001508         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142688_length_1326_cov_2.906373, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001508 DAORHD010000000
VERSION     DAORHD010001508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142688_length_1326_cov_2.906373"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1326)
                     /locus_tag="PKO06_23040"
     CDS             complement(<1..>1326)
                     /locus_tag="PKO06_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72603.1"
                     /translation="FQRLGEYTIEPGKRYIDCRFPRVAARFVRLTVLAHTGGNGFFLG
                     ECEVHGPTSVFGHFGVLHQSDWRNDTIRQVSLRDELVSGRDRGDPNHWPVSESNGCWV
                     GWREAGYTAAFVDTEDPENLGVTVYKRVGKGMIILETQELSYETNLPYSFWKANNLLD
                     LPPNPIQKGPSHTCLNIPSAGAYLLSVQASDANAWASRSYSLNFRFFPASDSFEPNET
                     PETAQRIATGIQETLYPFRDRDWWLFSLATPARYLYHLENLSLSLDPCLTVRAQAQDD
                     QELAQFAGSCRHGFGGDESGSVIATAPGLYLAEVRHSFPRSRSTDGYRLSISEKPINQ
                     SFEPNDTVKLAGRIDSDVPRRASIFPGTDQDWYVLDMATGTLDLTVVSEAEDLDPQIR
                     LYKPFVHKNGFISVLYLVGGMKSWHDFDQSLGTPFKVTRIEYDSPQARDV"
BASE COUNT          295 a          354 c          401 g          276 t
ORIGIN      
        1 acgtctcgag cctgaggact gtcgtattcg atacgtgtga ctttgaaagg ggtgccgaga
       61 gactgatcaa aatcgtgcca cgacttcatg ccgccaacga gatacaatac cgatatgaaa
      121 ccgttcttgt gaacgaaggg cttgtacaat cgaatctgcg gatcaagatc ttctgcttcg
      181 ctgacgacgg tgagatccag tgttcccgtc gccatgtcga ggacatacca gtcctgatct
      241 gtgccaggaa agatactggc ccggcgggga acgtccgaat cgatgcgacc tgcgagtttt
      301 accgtatcgt taggctcaaa cgattgattg atgggctttt ctgagattga caatctgtac
      361 ccgtcagtcg atcggctgcg cggaaatgaa tggcgcactt cggccagata caggccaggt
      421 gcggtggcaa tgacgctgcc agattcatcg ccaccgaacc cgtgccggca ggaaccggca
      481 aactgggcca gttcctgatc atcctgtgcc tgtgcgcgaa cggtcaggca ggggtcgagc
      541 gaaagcgaaa ggttctcgag gtggtacaga tagcgcgcgg gggtcgccag tgaaaacagc
      601 caccagtctc gatctctgaa cggatacagg gtttcctgga ttccggtggc gatccgctgg
      661 gctgtttccg gtgtctcgtt tggctcgaag ctgtcagaag ccgggaaaaa gcggaagttc
      721 agcgaatagg agcgggacgc ccaggcattc gcgtcgctgg cctgtaccga aagcaggtaa
      781 gcacctgccg aagggatgtt caaacacgtg tgactcggac ctttctgtat cggattgggt
      841 ggcaggtcga gcaggttgtt cgctttccag aacgagtagg gaagattcgt ttcgtagctg
      901 agttcctggg tttcgaggat gatcatgccc ttgcccactc gtttatagac cgtcacaccc
      961 agattttccg ggtcttccgt gtcgacgaat gcggcggtgt agccggcttc acgccagcca
     1021 acccagcagc cgttgctctc ggagaccggc cagtgattcg ggtcgccgcg atcgcgcccg
     1081 ctgaccagct catcacgcag agagacctga cgtatcgtgt catttcgcca gtcagactgg
     1141 tgcagaacgc cgaagtggcc gaagacggag gtcggaccat gaacctcgca ctcgccgaga
     1201 aaaaagccgt tgccaccggt atgagccaaa acggtcagtc ggacaaaacg agccgcgaca
     1261 cgtggaaagc ggcaatcgat gtaacgcttt ccgggttcga tcgtgtattc gcccagccgc
     1321 tgaaag
//
LOCUS       DAORHD010001509         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_142835_length_1325_cov_3.629134, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001509 DAORHD010000000
VERSION     DAORHD010001509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142835_length_1325_cov_3.629134"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKO06_23045"
     CDS             complement(<1..236)
                     /locus_tag="PKO06_23045"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HNV72604.1"
                     /translation="MPSASLKDLRFLSGIWRGELHGGTIEEQWSDPLGVCMMGMFRFV
                     KDGKPRFYEFMTLEETGAGLELRIKHFHPGLLGW"
     gene            complement(310..>1325)
                     /gene="ortB"
                     /locus_tag="PKO06_23050"
     CDS             complement(310..>1325)
                     /gene="ortB"
                     /locus_tag="PKO06_23050"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HNV72605.1"
                     /translation="AAQARQRGLKAVILQEVFDSRGIGQPEIVEKGRKCEAYGAEVHK
                     LTVGPELFYCLLTTLEETGFFNASLYTPFGIRGVETLGVELARQVRYRYHRDPDVVVI
                     THAGGGNVTGTARGLISAGCAQTRVVGCSVDLSGLHMASDTDFNRKSFTTGHTGFGVP
                     FATWPDRVDVPRNAARSLRYLDEYWLVNQGEVFYITELLAKLEGLERGPAGNTSLTAA
                     VALARQMDRDQLIVVQETEYTGAGKHHNSQLSFARKMGVEVRRGDPAQHDKPGVCICI
                     PERLSQVRGKTQEMDKLRLSYLKNAVKAYASDKWNDRDFAFLAADVKKPVSWVREQLS
                     GLK"
BASE COUNT          263 a          418 c          383 g          261 t
ORIGIN      
        1 tcccagccga gcaggccggg gtgaaagtgc ttgatgcgca gctcgagacc agcgccggtc
       61 tcttccagcg tcatgaactc gtagaaccga ggtttgccgt ctttgacaaa gcggaacatg
      121 cccatcatgc acacaccgag ggggtccgac cattgttcct cgatcgtccc cccgtgcagt
      181 tcgccacgcc agatgccact gagaaaacgc agatctttca acgatgctga cggcatgtca
      241 ggcctcctga atcgaaaccc ctcgaagttc tcttcgaggg gttttcactg aaatcatcga
      301 cgggaacgtc tacttcaagc cagagagctg ctcgcgcacc cacgacaccg gcttcttcac
      361 gtcagcggcg agaaaggcga agtctcgatc attccattta tcgctcgcat aagccttcac
      421 cgcatttttc agataggaca aacgcagttt atccatctcc tgtgtcttgc cgcgcacctg
      481 gctcagtcgc tcgggaatgc agatgcagac cccgggcttg tcatgctgtg ccgggtctcc
      541 ccgccgcacc tcaaccccca tcttgcgggc gaagctgagt tgcgagttgt gatgcttgcc
      601 ggcaccggtg tattcggttt cctgcaccac gatgagctga tcgcgatcca tttgccgggc
      661 cagggccacg gcggcggtca gactggtgtt gccggccggg ccgcgttcaa gcccttcgag
      721 cttggccagc agttcggtga tatagaacac ttcgccctga ttgaccagcc agtattcgtc
      781 gaggtagcgc agactgcgcg cggcattgcg cggcacgtcg acgcgatcag gccacgtggc
      841 gaacgggaca ccaaagccgg tgtgtccggt ggtgaacgac ttgcggttga aatcggtatc
      901 ggaagccata tgcaggccgc tgagatcgac agaacagccc accacgcgag tctgcgcaca
      961 acctgcactg atcaacccgc gcgccgtgcc cgtgacgttg ccgccaccgg catgcgtgat
     1021 caccacgaca tcggggtcac gatggtaccg gtagcgaacc tgtctcgcga gctcgacacc
     1081 cagggtttcg acaccacgaa tgccaaacgg ggtgtacagg ctggcgttga agaacccggt
     1141 ttcctcgagc gtcgtcaaga ggcaatagaa cagctcgggg ccgacggtca gtttgtgcac
     1201 ttcagcgccg taggcctcgc acttgcgacc tttttcaacg atctcgggct ggccgatgcc
     1261 acgcgagtcg aagacttcct gcaggatgac ggccttcaag ccgcgctgcc gcgcctgtgc
     1321 cgcga
//
LOCUS       DAORHD010001510         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_143030_length_1324_cov_3.121355, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001510 DAORHD010000000
VERSION     DAORHD010001510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143030_length_1324_cov_3.121355"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="PKO06_23055"
     CDS             complement(<1..644)
                     /locus_tag="PKO06_23055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNV72606.1"
                     /translation="MKSIWSRRGKKPDRSYQTLILTVLLLGVSFTAGAEGVRSGTLPR
                     GMESFDTVFPRLMREWGIPGGAVVLAKDGRLLLARGYGYANQDKRVPVDPWKHAFRLA
                     SLSKAITAAAILKLVESGRLKLDAPVFSLLPWVKSAELQVADPRLRQVTVRHLLHHSG
                     GWDAAQGYDPMFATRVIAEARKTSCPPSIRDIVHYMLTQSLSFDPGTAYAYSNFG"
     gene            complement(656..>1324)
                     /locus_tag="PKO06_23060"
     CDS             complement(656..>1324)
                     /locus_tag="PKO06_23060"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV72607.1"
                     /translation="YLLKKGVPVNASFTTGVGHGYTPLHAAAAAPYPDFAAKEVIELL
                     LTNGASLEERPIGGSTPLQEAAKSGSLDNLTTLLERGADPLVTDASGATVLHYLVSPL
                     SRTSTASVDLLLARGVPIDAATRDGKTPLFLAVSAGNRELAEHLLRKGASAAVVTGDG
                     ENLFHAMAAGYDFNPELFETLLAKGVSSSAKNRQGETPLALAQKNGFSQRAYQHFLKK
                     GFVP"
BASE COUNT          267 a          419 c          367 g          271 t
ORIGIN      
        1 ccgaagttgg aatacgcgta cgcggtcccc gggtcaaatg acaggctctg ggtcagcatg
       61 taatgaacaa tgtcacgaat ggacggcggg caggacgttt tccgtgcttc ggcgatcacg
      121 cgggtggcaa acatcggatc gtacccctgc gctgcgtccc acccaccgct gtgatgcagc
      181 agatggcgca ccgtcacctg tctcagtcgg ggatcagcga cctgcagttc cgccgacttg
      241 acccacggca gcagtgagaa taccggcgca tccagcttca gccgccccga ttccaccagc
      301 ttgagaatcg ctgccgccgt gatcgctttc gacagactgg ccaggcgaaa ggcatgtttc
      361 cacggatcga ccggtacacg tttgtcctgg ttggcataac cgtatcctct tgccagaagc
      421 agtcttccat cttttgcaag taccacagcc ccgccaggaa ttccccactc gcgcatcagt
      481 cgtgggaaca cggtgtcgaa actttccatc ccgcgaggca gcgttccgct tcgcacacct
      541 tcggcaccgg ccgtgaaaga caccccgagc agcagaacgg tcagaatcag cgtctgataa
      601 cttctgtcgg gcttctttcc tcttctcgac caaatgcttt tcaaaggagg agtcgtcatg
      661 gtacaaaccc ttttttcagg aaatgctgat atgctcgctg tgagaaacca ttcttttgag
      721 ccagagccag gggcgtctct ccctgtcgat ttttcgctga tgaagaaact ccctttgcca
      781 gaagggtttc gaagagttcc gggttgaaat cgtagcccgc ggccatggca tgaaacagat
      841 tctcgccatc gcccgtgacc accgcggcac tcgcgccttt cctcagcaga tgctcggcca
      901 attcacggtt acccgcagag acggcgagaa acaggggtgt cttcccatcc ctggtcgcgg
      961 catcgatcgg cacgccgcgg gccagcaaca ggtcgacgga tgccgtcgag gttcggctga
     1021 gcgggctcac gagatagtgc aacacggtcg cccctgaggc atcggtcacc agagggtcag
     1081 cgccacgctc cagcagtgtc gtgaggttgt ccaggcttcc gctttttgcc gcttcctgca
     1141 ggggcgtcga ccccccaatc ggccgttctt ccagcgacgc accgttggtc agaagcagtt
     1201 caatcacttc cttcgccgcg aaatccgggt aaggcgcggc ggcagccgca tgcagcgggg
     1261 tatatccatg ccccaccccg gtcgtgaacg aggcattcac gggaacaccc ttcttcagca
     1321 gata
//
LOCUS       DAORHD010001511         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_143201_length_1323_cov_3.261041, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001511 DAORHD010000000
VERSION     DAORHD010001511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143201_length_1323_cov_3.261041"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..577)
                     /locus_tag="PKO06_23065"
     CDS             complement(20..577)
                     /locus_tag="PKO06_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72608.1"
                     /translation="MSLTRSSSPCQTRFYRRILFLTAIWSLCLTTLFASPDFIPEPEA
                     VFTGAEAGAWARVERLSDPHPGFVQETFCPAGEQKDPSIVKWFGNKKTPHSRTFLLHC
                     AKGFDGRTLPEPILLVHGAGDNANRGWIHPYTSVLPEKLPLEERGLALWLSDLGYAVF
                     AVTFAHNQGCNIMQAEQVPLPGAKP"
     gene            1041..>1323
                     /locus_tag="PKO06_23070"
     CDS             1041..>1323
                     /locus_tag="PKO06_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV72609.1"
                     /translation="MVLWVAGWNNPGMLSHRESGERPEETKHQEKETRQMRLHDKVCV
                     ITGAGSGIGKAAALAFAREGGRVWAWDLNPDWLETLKAEAKGTIRGQVVD"
BASE COUNT          322 a          320 c          397 g          284 t
ORIGIN      
        1 cacatttggg aggcaatcat catggcttgg ctcccggcag cggcacctgc tcggcctgca
       61 tgatgttaca gccctgattg tgggcaaagg tcacggcaaa gacggcgtaa cccagatctg
      121 acagccagag agccaagccg cgttcttcca gggggagttt ttcggggagc acgctcgtat
      181 aggggtggat ccagccgcgg ttggcgttgt cgccggcacc gtgaaccagc agaatcggct
      241 cggggagcgt gcgaccatcg aaacccttgg cgcaatgcag taaaaaggtt ctggaatgag
      301 gcgtcttttt gttgccgaac cactttacaa tcgaaggatc tttctgctca ccagccggac
      361 agaaggtttc ctggacaaat cctgggtgag ggtcagacag tcgctcaacc cgcgcccaag
      421 cgcccgcttc cgcgccggta aaaaccgctt ctggctcggg gataaaatcc ggtgaggcga
      481 acagcgtggt cagacacagg gaccagatgg ccgtcaagaa cagaatgcga cggtagaatc
      541 gagtctggca agggctggac gatctcgtca ggctcacgct tccctccttg agtgggtctg
      601 tgacccggaa caactttgtc ttggctcccc agttttcaga aaaacttcga gcagataaaa
      661 gaaattataa gaaccaaagc cgggactgtc aactcccgcg ttcacacaga atttctgagt
      721 gtacgggaat gttggctatg cgcctctgcc ttgaggggct cttgcgctgc aaagtactcg
      781 tcgttgtctc ctgaaaagct cttgaataga gaatcttctg cgagaaatgc agatgcctgg
      841 ggatagaaaa ttttgcagtg caaaaattcg gcttgacagc cgataggttg agtggtactt
      901 tcacaagtaa ggaaagtgtt ggttacatcc atgctttcga accccagacg tgaaaatcgc
      961 agatatgaaa caggtttcgc gttgtctgaa aacaggtttt gtattttgcg gtgcagcgta
     1021 gtgaagcaag aggttatatc atggttctat gggtcgctgg ttggaataat ccgggtatgc
     1081 tctcgcatcg cgaaagcggc gaacgccctg aagaaacgaa gcatcaggaa aaggagacgc
     1141 ggcagatgag acttcacgac aaggtctgcg tgatcaccgg agccggttca ggtatcggaa
     1201 aagccgctgc tctggctttt gcgcgcgaag gtgggcgggt ctgggcctgg gacctcaacc
     1261 cggattggct ggaaacgttg aaagcggaag cgaaaggaac gattcgcggc caggtcgtcg
     1321 atg
//
LOCUS       DAORHD010001512         1322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_143416_length_1322_cov_2.501184, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001512 DAORHD010000000
VERSION     DAORHD010001512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143416_length_1322_cov_2.501184"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PKO06_23075"
     CDS             complement(<1..1167)
                     /locus_tag="PKO06_23075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72610.1"
                     /translation="MSTSHALMENAFRLSSVIDSSHDSLILFDANGRIYYMSAQAQQL
                     FGYGPGAYLHLDVRSLFVDVPETASPPLPLTQSPEQRFSSPREYVAVHHSGSRLPVDI
                     SVSQTELEPERIGYIAMIRDLSQTRRREVEFRQFRQAVEQSPVSVVITDLTGQIEYVN
                     PKFTEVTGYTSSEVIGKNPRLLNSGKTPPETYTSMWAALRNGNTWTGDFINKKKNGDS
                     YYEAASISTVRDHHGKPLFYVAVKEDVTQQRLAEEALRESQRFIRSTLDSLVDQVAIF
                     DVEGMLLDANASWRQHHPAPEPGRRITLDACLGEAFTEDQPRQLARQAAHDIAARRRE
                     RFASEVVLGTPARWYRLELSPFQGDGPMRLIFTLHDIDTQQQAMRALVKARDEAE"
BASE COUNT          261 a          373 c          410 g          278 t
ORIGIN      
        1 ttctgcctcg tcgcgggcct tcaccagcgc gcgcatggcc tgctgctgcg tgtcaatgtc
       61 atgcagagtg aagatcaggc gcatgggccc gtccccctgg aatggggaga gttccaggcg
      121 gtaccaccgt gcgggtgttc ccagcaccac ttcgctggcg aaccgctccc ggcgtcgggc
      181 ggcaatatcg tgcgctgcct gccgagccag ctggcgtggc tggtcttcgg tgaaggcttc
      241 cccgagacag gcatcgaggg tgatacgacg tccgggctca ggagccgggt ggtgctgccg
      301 ccaggaggca ttcgcatcga gaagcatgcc ctcgacatcg aagatcgcca cctgatcgac
      361 cagcgagtcg agggtcgagc gaatgaatcg ctgactttct ctcagggcct cttctgccag
      421 acgctgctgg gtgacatctt ccttgaccgc gacatagaaa agcggtttcc cgtgatgatc
      481 ccggacagtc gaaatcgaag ccgcttcata gtacgagtca ccattcttct ttttgttgat
      541 gaaatcacct gtccaggtgt tgccattcct taaagccgcc cacatcgagg tgtaggtttc
      601 cggcggcgtt tttccggagt tgagcagacg tggatttttc ccgatgactt ccgatgaagt
      661 gtacccggtc acctcggtga acttcgggtt cacatactcg atctgccctg tcagatcggt
      721 gatgacgaca ctgacggggc tttgctcgac cgcctgccga aactggcgaa actcgacctc
      781 acgtcgccgc gtctgcgaga ggtcgcgaat catcgcgatg tagccgattc tctcgggttc
      841 gagttcggtc tggctcacgc tgatgtcgac tggtagacgc gatccggaat gatgtacggc
      901 gacgtattcg cgcggtgacg aaaaacgttg ttccggcgac tgcgtgagag gaagcggggg
      961 tgacgcggtt tcgggcacgt cgacaaacag ggagcgaacg tcgagatgca gataagctcc
     1021 gggaccgtag ccgaaaagtt gctgggcttg cgcgctcatg tagtagatac gcccattcgc
     1081 gtcgaagaga atcaggctgt catgcgaact gtcgataact gatgacagtc tgaaggcgtt
     1141 ttccatgagt gcgtgacttg tactcatcag ctgatcggcc gaggccaggc tatcggcttc
     1201 cagttgtcga acgcgctccc gaagatgatt ctgctcttgg cggagtccct ggaaggacag
     1261 ccagatgccg cccaggagga gcaggccgat cacgaaccag agcaacaccc acggggcgaa
     1321 ca
//
LOCUS       DAORHD010001513         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_143941_length_1319_cov_3.255538, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001513 DAORHD010000000
VERSION     DAORHD010001513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143941_length_1319_cov_3.255538"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..>1319
                     /locus_tag="PKO06_23080"
     CDS             172..>1319
                     /locus_tag="PKO06_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72611.1"
                     /translation="MTTASAQPNFSSPQDELDHLRHLQSIAKEIDSLIEQCIVSQKCL
                     AATVEDVFSFVATKLKPQAQVLRSTDEQLLLATYTRNATPAMVTLLEEEALRTNDRCH
                     KIIDGISWYALSLDMAGESVGVFALGWPVATALQQERIFDCMNIIVEELDSFFFAIRA
                     NRFKHMTILEIQRCLKSKNLLTAIDQAINILGDAVPFEDLLLLYLDEDLEGTNNIQYT
                     LYRHCSKAFDSQEKPMPELEALIREGKEIVIPGNRDLQKIIEIDGSTETVLIDGLVVE
                     TLVGKLLVKPPTGIGLSVSSREILQVFAEALRQRLVDFNREKNLLRQYFSPVVTRQLL
                     QIPDYKNQFLTPRKADIGILFADVSGFTKLSEQVLREPERIARFIDQWA"
BASE COUNT          312 a          378 c          335 g          294 t
ORIGIN      
        1 ttctctgttg gatctgccgt ggcaatggca tcctgacgat tcgcgtatcc ccaaaaaatc
       61 cgcccggaaa cagcagagta ggctggatac aggggagttg ggatgacgat gcttttctga
      121 tagaatgcgg ctgatcattc atttggaatc acagagacta aggaaggtcc catgaccacc
      181 gcatcagccc agccgaactt ctcttctcca caggacgaac tcgaccatct gcgccatctg
      241 cagagcattg ccaaagaaat cgactccctg atcgagcagt gcatcgtcag tcagaagtgt
      301 ctggcggcta cggtggaaga cgtgttttcg tttgtcgcga caaagctcaa accacaggct
      361 caggttctgc gttccactga tgaacaactg ttactggcca cctatacccg aaacgcgacc
      421 ccggccatgg ttacgcttct ggaagaagaa gcgcttcgca ccaacgatcg ctgtcacaag
      481 atcatcgatg gaatctcgtg gtatgcactg tcgctcgaca tggccggcga gtccgtcggt
      541 gtcttcgcgc tcggctggcc tgtagcaact gcgctccagc aggaacgaat cttcgactgt
      601 atgaacatca tcgtcgaaga gctcgacagc ttctttttcg ccattcgtgc caatcgcttc
      661 aagcacatga ccattctgga aattcagcgc tgcctcaaat caaagaatct gctgaccgcg
      721 atcgatcagg ccatcaatat cctcggtgat gcagttccat tcgaagatct tctgcttctg
      781 tacctggacg aagacctcga gggcacgaac aacattcaat ataccctgta tcggcattgc
      841 agcaaggcct tcgactccca ggaaaaaccg atgcccgaac tcgaagccct gatccgcgaa
      901 ggcaaagaga tcgtcattcc cggcaatcgt gatctgcaga agattatcga gatcgatggg
      961 tcaacggaga cggttctgat cgacggcctg gtcgttgaaa ccctggtcgg caagctcctg
     1021 gtcaaaccac cgaccggcat cgggctctcg gtctcaagtc gtgagattct gcaggtcttc
     1081 gccgaagctc tgcgacagcg gctggtcgat ttcaaccgcg agaaaaacct gctgcggcag
     1141 tatttctccc cggtggtcac tcgccagctg ctccagattc ccgattacaa gaaccagttt
     1201 ttaactccgc gcaaagccga tatcggcatc ctgtttgccg atgtctcagg tttcaccaag
     1261 ctgagcgaac aggtgctgcg cgaacccgaa cgcattgcgc ggtttatcga tcagtgggc
//
LOCUS       DAORHD010001514         1319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_143990_length_1319_cov_2.553006, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001514 DAORHD010000000
VERSION     DAORHD010001514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143990_length_1319_cov_2.553006"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PKO06_23085"
     CDS             complement(<1..871)
                     /locus_tag="PKO06_23085"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNV72612.1"
                     /translation="MTNGLILAIFGFWILVLLYIALRGWGLSWAILTAALGLGTSLGQ
                     FTHLPAAAWTVTLDPQVSELLAVIILIAIFSQLLRDSHRMEGVSEYLIRVLQSPRWVL
                     IAVPALVGLIPMPGGAMFTAPITDEVGNTIKLKPADKVFSNYWFRHCWELAFPLYPGI
                     ILAAGLMKVSPAELAWRLMPLAVAALAGGVLVFWHNSGKHGNQEMLHRAENNVSWLVL
                     WPILAVVGLAVVRLPLIPGLAGIILWYALAERIPWRRFVQTCREAVQWPIVLLVWAVF
                     LFGRELQDTGVLSA"
     gene            complement(913..>1319)
                     /locus_tag="PKO06_23090"
     CDS             complement(913..>1319)
                     /locus_tag="PKO06_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72613.1"
                     /translation="IDRADRSRDDTEIMLAMAHASFLHWQNRPDCKPENLSIAYWLLA
                     RVHALAGHFEAARWYASKCLSYSSDKALAPFYIGYAHEAMCRAEKGLKETEAALGDLK
                     LAEGQLEKVTDPEEKKLLADDLRSLREALATP"
BASE COUNT          303 a          410 c          378 g          228 t
ORIGIN      
        1 gagctgacag gactccggtg tcctgtagtt ctcgaccgaa caggaacacc gcccacacca
       61 gcaaaacaat gggccactgc acggcttcac gacatgtctg cacgaatcga cgccagggaa
      121 tgcgttcggc cagagcatac cagagaatga ttcccgccag accgggaatc agcggcagtc
      181 tcaccaccgc caggccgacc acggcaagta tgggccagag taccagccag ctgacgttgt
      241 tctctgcccg gtgaagcatc tcttgattgc catgttttcc tgaattgtgc caaaagacga
      301 gcaccccacc cgcgagagcc gccacggcca gtggcatcaa ccgccaggcc agttcagcag
      361 gcgacacctt catcaggccg gcagccagaa tgatcccggg atacagcgga aacgccagtt
      421 cccagcagtg tcgaaaccag tagttggaaa agaccttgtc cgccggtttc agcttgatcg
      481 tgttccccac ttcgtcggtg atcggagcgg tgaacatggc cccgcccggc atgggaatca
      541 ggccaaccag cgccggaacc gcgatcagga cccagcgcgg actctgcagc acgcgaatga
      601 gatactcgga caccccttcc atgcgatgtg agtcccgcaa gagttgcgag aagatggcga
      661 tgagaatgat cacggccagc agctccgaca cctgcgggtc aagggtgacc gtccaggcag
      721 ccgcgggcag gtgggtgaac tgtcccagcg aggttccaag accgagtgcg gctgtcagaa
      781 tggcccaact gagaccccag ccgcgcaagg caatgtacag caaaacgaga atccagaaac
      841 cgaagatcgc cagaatcaaa ccgttggtca tgggtcacag gggaacgact cgcccgagtc
      901 tcccgcaggc tgtcagggtg tcgccagcgc ttccctgagt gagcgcagat cgtccgcgag
      961 caatttcttt tcttcagggt cagtcacctt ctcgagctgc ccctcagcca gtttcaggtc
     1021 gccaagcgcc gcttcggtct ctttcagtcc cttttcggcg cgacacatgg cctcgtgtgc
     1081 atacccgatg taaaacggtg ccagcgcctt gtcgctcgaa tacgagagac acttggaagc
     1141 gtaccagcgc gcggcctcaa agtgcccagc caatgcgtga acgcgggcaa gcaaccagta
     1201 ggcgatcgac aggttttcag gcttgcagtc tggtcgattc tgccagtgca gaaatgaggc
     1261 atgagccatc gccagcatga tctctgtatc atcgcgcgag cggtcagcgc gatcgatca
//
LOCUS       DAORHD010001515         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_144518_length_1316_cov_3.365583, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001515 DAORHD010000000
VERSION     DAORHD010001515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144518_length_1316_cov_3.365583"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1316)
                     /locus_tag="PKO06_23095"
     CDS             complement(<1..>1316)
                     /locus_tag="PKO06_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72614.1"
                     /translation="WQSLAIPVSLSFALLFPPLLEAGAMDWLQGKLGAKPRSSAAPAE
                     ESGAKPRNSAPAAENATSLPDFFAPSDRLEATGQTLSTLKFVEAHWTHSRQVLETVSR
                     SLAAKVQIKRCSDISAKLFQIVTGLSKESLKNSGGAIQGIAQSARELEQQLLLLGRII
                     TAPGSETLANLESSIQKARTINDRQFSLVKSYLTGAESLISLGSEAYQTLELIPLLSI
                     NALDTYIAGGKQVMKQIRSNGEAMKGLLLNLQTGCEQVNAGLELMTTTLKTTLRFSDH
                     FAFKQFALINLPVPSREKLFSQVGMLKNSLKGMQNTLEIGDSQVKNSTQQYAHLVEEI
                     TSKIRDHLQYQTAVDAASGNLPQISTYAHNQVLGLFQRAKQGIAEMRVGMNRTNREAL
                     NARPPQVAMESSTDSQARVAERVAAGKLPLFLLGGKAATSVSTATP"
BASE COUNT          268 a          348 c          401 g          299 t
ORIGIN      
        1 gggtgtggcc gtagagacag aggttgccgc cttgccgccg agaagaaaca acggaagttt
       61 gcccgcggcg actcgctcag caacgcgtgc ctgagagtcg gtcgaacttt ccatggcgac
      121 ttgcggcggc cgcgcgttca gcgcttcacg gttcgtgcgg ttcatcccaa cccgcatctc
      181 cgcaattccc tgctttgcac gttgaaacag gccgagaacc tgattgtgcg cgtaggttga
      241 gatctggggc agattcccgg aagccgcgtc gaccgcggtc tgatactgaa gatgatcacg
      301 aatcttgctg gtgatttcct cgaccagatg tgcatactgc tgcgtcgagt tcttgacctg
      361 ggaatctccg atttcgagcg tgttctgcat gcctttcagc gagttcttca acatgccgac
      421 ctgcgaaaac agcttctcgc gcgagggaac gggaaggttg atcaaggcaa actgtttgaa
      481 ggcgaagtga tcagaaaagc gaagcgtcgt cttcagagtg gttgtcatca gttccagacc
      541 ggcgttgacc tgttcacagc cggtctgcag gttcagcagc agccccttca tggcttcgcc
      601 gttggaacga atctgtttca tgacctgctt gccgcccgcg atgtaagtgt cgagcgcgtt
      661 gatggaaagc agggggatca actcgagagt ttgataggct tctgacccaa gactgataag
      721 agattccgct ccggtaaggt aggactttac cagactgaat tgccggtcat tgatggtgcg
      781 ggctttctgt atcgagcttt cgagattcgc cagtgtttct gaaccagggg cggttatgat
      841 gcgtccgagt agcaataact gctgttcaag ttctcgagcg ctttgagcaa taccctgaat
      901 cgcacccccg gagtttttca gcgactcttt cgacaagccg gtcacgatct gaaacagctt
      961 ggcggaaatg tcagaacagc gcttgatctg caccttggcc gccagggaac gggatacggt
     1021 ttcgagaacc tgtcgtgagt gcgtccagtg cgcttcgacg aatttcaggg tgctcaacgt
     1081 ctggccggtg gcctcaagtc ggtcgcttgg ggcgaagaag tccgggagag acgtggcgtt
     1141 ctctgcggct ggggccgaat tcctgggctt ggcgccggat tcttcggcgg gagctgccga
     1201 tgatctcggt ttcgcgccaa gtttcccctg cagccaatcc atggcgcccg cttcgagaag
     1261 cgggggaaac agcagagcga aactcagaga tacgggaatg gccagagact gccacc
//
LOCUS       DAORHD010001516         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_144529_length_1316_cov_3.222046, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001516 DAORHD010000000
VERSION     DAORHD010001516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144529_length_1316_cov_3.222046"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="PKO06_23100"
     CDS             <1..923
                     /locus_tag="PKO06_23100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV72615.1"
                     /translation="MESELFGYEKGAFTGATGSKPGVFELAQGGTLFLDELGDMSLEM
                     QAQLLRVLEEREIVRVGGNKKIKVDVRIIAATNKDLRKYIAENRFRDDLFHRIKVIEI
                     KLPPLRERVEDIPALARHFMAMLATQRQGPAKTLSDVAIEALCAFPWPGNVRELRNAV
                     DQAYTLSRDQIMLPEHLPAEIRAISTGMPMPSVRPSEPVAVHAPAVSPPTVGVPVPPL
                     PVMPPGQPSREPVDSAPAASLQSMAEMFVRSHLKEKISGDAYDAVLGMVEKELLNQTL
                     ILFKGNQVQTANYLGITRNTLRAKIEKYGL"
     gene            complement(1003..>1316)
                     /locus_tag="PKO06_23105"
     CDS             complement(1003..>1316)
                     /locus_tag="PKO06_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72616.1"
                     /translation="REDILADPSEGFRDDGSVQANLMNPLMAEFSRRDAQEQLIADTV
                     AQDIAKGDTGILEDFKALYQAADAPTRKKLAGLKQKLNDVVRFEIMQNGHTELQPYLI
                     E"
BASE COUNT          281 a          371 c          381 g          283 t
ORIGIN      
        1 taatggagtc cgagttgttc ggctatgaga agggcgcctt taccggagca acaggttcaa
       61 aacccggtgt gttcgaactg gctcaaggcg gaaccctgtt tctcgatgag ctcggtgaca
      121 tgagcctcga aatgcaggcc cagttgctgc gcgtccttga agaacgcgaa atcgtccgtg
      181 tcggggggaa caagaagatc aaggtcgatg tgcgtatcat cgcggcgacc aacaaagatc
      241 tgcggaagta cattgccgag aatcggttcc gcgacgatct gtttcaccgc atcaaggtca
      301 tcgaaatcaa gttgccccct ctgcgcgagc gggtcgaaga tataccggct ctcgcccgtc
      361 acttcatggc catgctggcc acacaacgtc aggggccggc gaagacgctg tcggatgtcg
      421 cgatcgaagc gctgtgcgca tttccgtggc cgggcaacgt gcgtgaactg cgcaatgcgg
      481 tggatcaggc ctataccttg agtcgtgatc agatcatgct gccggaacac ctgccggcgg
      541 aaattcgtgc gatttcgacc ggaatgccca tgccgagcgt tcgcccgagc gaaccggtcg
      601 ccgtccatgc cccggccgta tcgccgccta ccgtcggggt tccggtgcct cccctgccgg
      661 tcatgccgcc gggacaacct tcgcgcgaac ccgttgattc ggctccagcc gcctctttgc
      721 agagcatggc ggagatgttt gtccgatctc atttgaaaga aaagatctct ggtgatgcat
      781 atgatgccgt acttggcatg gtggaaaagg aactgctcaa ccagacgctg atcctgttca
      841 agggcaatca ggtgcagacc gcgaactatc tcggcatcac gcgaaatact ctgagggcga
      901 aaatcgaaaa gtatgggctc taaaaagtct gaagatcagc agctgcaata aaaacggccc
      961 ggacaatcgt ccgggccgtt ttacgatgac gcgatgtgcg atctactcga tgagataggg
     1021 ttgcagctcc gtatggccgt tctgcatgat ttcaaatcgg acaacatcat tgagcttctg
     1081 cttcagaccg gcaagtttct tccgggtcgg agcatctgca gcctgataca gggctttgaa
     1141 gtcttccaga atgccggtgt cgcccttggc gatatcctgg gccaccgtat cggcgatcag
     1201 ctgttcctgg gcatcacggc ggctgaactc cgccatgagc gggttcatca ggttcgcctg
     1261 caccgaaccg tcgtctcgga acccttctga cggatcagcg agtatgtctt cccgct
//
LOCUS       DAORHD010001517         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_144578_length_1316_cov_2.469469, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001517 DAORHD010000000
VERSION     DAORHD010001517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144578_length_1316_cov_2.469469"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1316)
                     /locus_tag="PKO06_23110"
     CDS             complement(<1..>1316)
                     /locus_tag="PKO06_23110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72617.1"
                     /translation="PGLAEVGDAGGSADELDEAALRRYRERKLRLLDSVAEALLQLNP
                     AQEHVQEFKALIETLKTKVEEAHTVAELHRLFHAESSLLLRFAEMVARVLVKAGFTVD
                     MMRECFNVPVPEYGFAGVTGLDADENAKQTLPALLNASADPAGAANGFLARQEQVLSE
                     LIAVVRKVALAHQVTLSEVQLKAVALLIWVGGAENLNFTLPLPPIHTDPTPISYTLTG
                     TVVKLESAQILKEQAFKYALQAETVTYAVDGAEKCSPAAPCTIAYLTEKDRITLIKID
                     ANGKRTRSLATLEDCVGAGLVEVQGDLAAVPWKFDDPMPRLPEGAEPAVGTANSGASS
                     GSNGDGSSAGTVDVVPVDPAHPLPWVADPLYVVVRAATILPPPPPPPMRIDGVIGTLA
                     HVTSDGTRIAGVYVFKADDATSPCHNAVARFALRWLDSDNWPEFST"
BASE COUNT          266 a          409 c          393 g          248 t
ORIGIN      
        1 ggtggaaaac tcgggccagt tgtccgaatc cagccagcgc agcgcaaaac gggcgaccgc
       61 attgtgacag ggcgaggtcg cgtcatctgc tttgaagaca tagactccgg cgatgcgggt
      121 tccatctgaa gtgacatgcg ccagagtgcc gatcacgcca tcaatgcgca tggggggtgg
      181 cggcgggggc ggaagaatcg ttgcggcgcg cacgacgaca tacagcggat cagcgaccca
      241 cggcagcgga tgcgctggat cgaccggtac gacatcaact gtaccggctg atgaaccgtc
      301 accatttgac ccagagcttg ccccactgtt ggcagtcccg acggcgggtt cggcaccctc
      361 gggcagtctc ggcatcggat catcgaactt ccaggggacc gcggccagat ctccctgcac
      421 ttcgacgaga ccggcaccaa cacaatcttc aagcgtggcc agactcctgg ttcgttttcc
      481 gttcgcatcg atcttgatca acgtgatccg gtctttctcg gtaagatacg caatcgtgca
      541 cggagcagct ggcgagcact tttccgcacc gtcaaccgca taggtcacag tctctgcctg
      601 cagggcatac ttgaacgcct gttccttcag aatctgcgcc gactcgagtt tgacgaccgt
      661 accggtcagc gtgtaggaga tcggtgtcgg atccgtgtga atcgggggca gaggcaaggt
      721 gaagttgagg ttttccgcgc caccgaccca gatgagaagc gccacggctt tgagttgaac
      781 ctcgctcagg gtcacctgat gagccagggc gaccttgcgc acgacggcga tcaattcgct
      841 gagaacctgt tcctgtcggg ccagaaagcc atttgccgcc cccgcggggt cagccgaagc
      901 attgagcagg gcaggcaagg tttgcttcgc gttctcgtcc gcatctaaac cggtcactcc
      961 ggcaaaacca tactccggaa ccggaacgtt gaagcactcg cgcatcatgt cgacggtgaa
     1021 accggctttc acgagcaccc gggccaccat ctcggcaaag cgcagcagca gactgctttc
     1081 ggcgtgaaac aaccgatgca gttccgccac ggtgtgcgcc tcttccacct tcgtcttcag
     1141 cgtctcgatg agcgccttga actcctgcac atgctcctga gcaggattca gctgcaacaa
     1201 cgcttcagcc acggagtcga gcaaccggag cttgcgttcg cgataacggc gcagcgcggc
     1261 ttcgtccagc tcgtcggcag agccgccggc gtcgccgact tcggcgagtc cgggct
//
LOCUS       DAORHD010001518         1314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_144900_length_1314_cov_3.664813, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001518 DAORHD010000000
VERSION     DAORHD010001518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144900_length_1314_cov_3.664813"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PKO06_23115"
     CDS             <1..683
                     /locus_tag="PKO06_23115"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNV72618.1"
                     /translation="LEMGPTWSRFDLVAEGVRQRCAIHLPGPFNIFNAALASGLARTL
                     EIPWSEIVAGLDNLKQVPGRFESIPNERGLTVIVDYAHSPASLENVLRAVRAMTSKRV
                     ISIFGCGGNRSADKRPIMGRLSASLADITIVTSDNPRKEDPQEIVRQIMAGIEQLPSS
                     QRREVCVEVDRRRAIEKGLSMALSGDVVLIAGKGHETGQTFADRTVPFDDREVAREIL
                     QLKEGKHV"
     gene            676..>1314
                     /gene="murF"
                     /locus_tag="PKO06_23120"
     CDS             676..>1314
                     /gene="murF"
                     /locus_tag="PKO06_23120"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNV72619.1"
                     /translation="MSDWTLEQIATAMQGAVEGAPRQNLEVTGFSIDSRTVKPGEVFV
                     AIKGENHDGHRFIPDVLQKEVSAVVASASATLPVDRNGVPVVRVGDCLNALQKLAGWH
                     RKRHPGLFLGITGSNGKTTTKEMLAHLFSRVSSLWATAGNLNNHIGLPLNLVRIPLSC
                     QTAIMEMGMNHAGEIRFLAELARPTSGIITGIGPAHIGNLGSLENIALAKAEM"
BASE COUNT          273 a          343 c          402 g          296 t
ORIGIN      
        1 cactggagat gggcccgacc tggagtcggt ttgatctggt tgccgagggc gtgaggcaga
       61 gatgcgcgat tcacttgccg gggccgttta acatctttaa tgcagcgtta gcttctggac
      121 tggctcggac tctcgagatc ccctggtccg aaatcgtggc gggcctcgac aatctcaagc
      181 aggtgcctgg gcgtttcgaa tcgattccga acgagcgggg tctgacggtt atcgttgact
      241 acgcgcactc gccggcgtcg ctcgagaatg tgttgcgggc ggtgcgtgcc atgacttcca
      301 aacgggtgat ttcgatcttc ggctgtggag gaaaccgatc ggcggacaag cgaccgatca
      361 tggggcgcct ctcggcttca ctggctgata tcaccattgt gaccagtgac aacccccgga
      421 aggaagatcc acaggagatt gtgcggcaga tcatggcggg aatcgagcaa ctcccatcgt
      481 ctcaacgtcg agaggtttgt gttgaggtag atcgacgtcg ggcgatcgag aaagggctct
      541 ccatggcgct ttcgggcgat gtcgtcttga tcgccggcaa aggacatgaa accgggcaga
      601 cgttcgccga tcgcacggta ccattcgatg atcgggaagt tgctcgtgaa atcctgcaat
      661 tgaaggaagg caagcatgtc tgactggacc ttggaacaga tcgccaccgc gatgcaggga
      721 gccgttgaag gggctccgcg tcagaacctt gaagtgacgg ggttttccat cgactcccgc
      781 accgtcaaac caggcgaagt atttgtagcc atcaaaggag aaaatcatga tggccatcgc
      841 ttcatccctg atgttcttca gaaggaggtc tcagcggttg tcgcctctgc gtcagcgacg
      901 ctacccgtgg accgtaacgg ggttcctgtt gtccgagttg gggattgcct gaatgctctt
      961 caaaagctgg caggctggca tcgaaaacga caccctggtt tgtttctcgg catcactggc
     1021 agtaatggga agaccacgac caaggagatg ttggcgcatc tcttttctcg cgtctcttcg
     1081 ctttgggcca ctgctggaaa tctgaataac catattggtc ttcccctgaa tctggtgcgt
     1141 attccgttgt cgtgccagac ggcgatcatg gagatgggca tgaaccatgc tggcgagatt
     1201 cgctttctcg cggagctggc tcgacccact tcgggaatca tcacgggtat tggtccggcg
     1261 catatcggca acctgggcag tctcgaaaac atcgcgctgg ccaaagcgga aatg
//
LOCUS       DAORHD010001519         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145100_length_1313_cov_3.589825, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001519 DAORHD010000000
VERSION     DAORHD010001519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145100_length_1313_cov_3.589825"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKO06_23125"
     CDS             <1..235
                     /locus_tag="PKO06_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72620.1"
                     /translation="TVIGPPGTKEFAVSWGEVTEVDGDALDMSHVAGYANLLLGWWEP
                     GIMAHQGNGPFGDALLTAGYLLEAWASHPPKQG"
     gene            complement(315..644)
                     /locus_tag="PKO06_23130"
     CDS             complement(315..644)
                     /locus_tag="PKO06_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72621.1"
                     /translation="MKFDGRKPTHRDVLDRFLAEFETIWNSSMTVDAWKGRKLAERIV
                     RTLENQDVSYVRGTIERRPGMTIDQLVERLKRSRSAIASELGKLVAANLVKMTHQGDE
                     LSFELTD"
     gene            complement(644..>1313)
                     /locus_tag="PKO06_23135"
     CDS             complement(644..>1313)
                     /locus_tag="PKO06_23135"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV72622.1"
                     /translation="ANGSANLCELTDKVYAEDRVVFFDDPEVSKAFAQEFARLWELYG
                     EPAYGTITPEKLDNSRINSDVRIVFTSLKDKPRAMTVDKLLEKTLDQVDSRRGTIDIA
                     LFAYTHYGITQKLLQMAEAMPKVKIRILLDHSQLRSEGDKKGLMSEYLEREIRARRLK
                     NIEIRYKFRANAYGFDKEAKQNMLVHVRSYLMHHKCLFVNRRYLVTGSYNWSESASER
                     NLEM"
BASE COUNT          260 a          365 c          393 g          295 t
ORIGIN      
        1 aaccgtcatc ggtccgccgg gaaccaagga attcgcggtc tcgtggggtg aagtgaccga
       61 ggtcgacgga gacgccctcg acatgagcca tgtcgccggg tacgcgaacc tgctgctggg
      121 ctggtgggaa cccggaatca tggcacatca gggcaacggc ccgtttggtg atgcgctgct
      181 gacggccggg tatctgctcg aagcctgggc ttcacacccg ccgaaacagg ggtaagcgcc
      241 ggcaacatcg ggcgaagaga acgaaaaaaa acaacgggtg accatcgggt cacccgttgt
      301 gcgtatgagt cagatcaatc ggtcagttcg aaactcagct cgtctccctg gtgggtcatt
      361 ttcacgaggt tggccgcaac cagtttgccc agttcagagg caatggcgct gcgtgagcgc
      421 ttcaagcgct cgacgagctg atcgatggtc atgcccggtc ggcgttcgat cgtgcctcgc
      481 acatacgaaa cgtcctggtt ctcgagcgtg cgcacaatcc gttcggccag ttttcgcccc
      541 ttccaggcgt caaccgtcat cgacgagttc cagatggttt cgaactcggc taagaaccgg
      601 tcgagcacgt cacgatgcgt cggcttgcga ccgtcgaact tcatcacatt tcgagattgc
      661 gttcagaggc cgattctgac cagttgtagc tacccgtcac cagatagcgg cgattgacga
      721 acagacactt gtgatgcatg agatagctgc gcacatgcac gagcatgttc tgcttggcct
      781 ctttgtcgaa gccgtaggcg ttggcgcgga acttgtagcg aatctcgatg ttcttcaggc
      841 gccgcgcgcg aatttcgcgt tcgagatact cgctcatcag gcccttttta tcgccttctg
      901 aacgcaactg ggaatgatcg agcagaatgc ggatcttcac ctttggcatg gcttcggcca
      961 tctgcagcag tttctgggtg atgccgtaat gcgtataggc aaagagcgca atatcgatgg
     1021 tgccgcggcg cgagtcgacc tgatcaaggg tcttttcgag cagcttgtcg acggtcatgg
     1081 ctcgcggttt gtccttcaga ctcgtgaaca cgatgcgtac atccgagttg atgcgactgt
     1141 tgtcgagctt ctcgggggtg atcgttccgt atgccggttc tccgtagagt tcccacagcc
     1201 gcgcgaactc ctgcgcgaac gccttgctga cttcagggtc atcgaagaag acgacgcggt
     1261 cttccgcata gactttgtcg gtcagttcgc agaggtttgc cgaaccattg gcg
//
LOCUS       DAORHD010001520         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145265_length_1312_cov_3.546539, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001520 DAORHD010000000
VERSION     DAORHD010001520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145265_length_1312_cov_3.546539"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="PKO06_23140"
     CDS             <1..1258
                     /locus_tag="PKO06_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72623.1"
                     /translation="NTYLLGGSSDDKCRNTAAPSKSPVTTEVQQQTQDDFFQFPLEQQ
                     LKLLNDWNPATGSDALPVLKKLVETRSTSPMLIAAALRAAIRLDVRLFAGRAEEYLRQ
                     TDMNLVNASLEYMAFADLDKILPTIDRYLTHQQPRLRSTAVLIFKRADPHQALASMQN
                     LLRHRDLDKQEAALAGLIHFDFSLVREMLTQYLRSCKSKRAFESGLCLFQANPDPENL
                     FSLEVLEPAVLNEFKIEVRKARDFIEAAILDSGLMDSQALQAFKSQFPQRFSRQQARL
                     ALKSQGVSAEGKTSNVKISPDTLLEKLGEFKGVFAVVTICLIVFCIGLTSRSSETTST
                     SGQVAVSSVKLGSELRTVITVMEIDPKKGGMVLEDRENNRFLVFPQQDGFTTPSLKST
                     WQVKLVPFRRGDQGIIHARLQELTKP"
BASE COUNT          304 a          389 c          338 g          281 t
ORIGIN      
        1 gaatacctat ctcctgggcg ggtcgtcaga tgataaatgc cgaaacacgg cagcaccgtc
       61 aaagtctccc gtcacgaccg aagtgcagca acaaacccag gatgattttt tccagttccc
      121 cctggagcag caattgaagc ttctcaatga ctggaatccc gcgacgggaa gtgatgcgct
      181 cccggtgctg aaaaaactgg tcgaaacacg gtcaacttct cccatgctca tcgcagcagc
      241 cctgagggcc gccattcggc tcgacgtccg tctgtttgcc ggacgcgccg aggaatacct
      301 tcgccaaacc gacatgaatc tggtgaacgc gtctctcgaa tacatggcct ttgctgattt
      361 agataagatt ctgcccacaa tcgacaggta cttaacccat cagcagcccc gccttcgctc
      421 aacggctgta ctgattttca aacgggcaga ccctcatcaa gcgctcgcct ccatgcagaa
      481 cctgttacgg catcgcgacc tcgacaaaca ggaggccgcg ctggccggac tgatccattt
      541 cgatttctcg ctggtgcgtg aaatgctcac gcagtatctc cgctcctgca agtcgaaacg
      601 ggccttcgaa agcgggctgt gcctgtttca ggccaacccg gatccggaga atctgttttc
      661 tctcgaagtg ctcgaacccg cggttctgaa tgagttcaag atcgaggtcc gcaaggcgcg
      721 cgactttatc gaagcggcca ttctggacag cggcctgatg gacagtcagg ctctacaggc
      781 tttcaagtcc cagtttccgc aacgtttttc gcgccagcag gcacgactcg ccctcaagtc
      841 gcagggagtc tctgcggaag gaaagacctc caacgtcaag atttcgccgg ataccttgct
      901 ggaaaaactt ggagaattca aaggcgtttt cgcggtcgtg acgatctgtc tcatcgtgtt
      961 ctgcatcggt ctgacatctc gttcgtcaga gacgacttcc acctccgggc aggttgccgt
     1021 ttcgtcggtg aaactcggct ccgagttgcg caccgtcatc acggtcatgg agatcgaccc
     1081 caagaagggg ggcatggttc tggaagaccg cgagaacaat cgttttctgg tgttccccca
     1141 acaggatggc ttcacaaccc cttccttaaa gagcacctgg caagtcaaac tggtaccatt
     1201 ccgtcgcggc gaccaaggca ttatccatgc ccgattgcag gaactgacca aaccgtagcg
     1261 tcaagaggga aacatgagct cgttcaccat ttcacacgaa gagaacacgg ga
//
LOCUS       DAORHD010001521         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145325_length_1312_cov_2.583134, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001521 DAORHD010000000
VERSION     DAORHD010001521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145325_length_1312_cov_2.583134"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1312
                     /locus_tag="PKO06_23145"
     CDS             <1..>1312
                     /locus_tag="PKO06_23145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72624.1"
                     /translation="LFWDIASPSYNQRTYVLTGFESMGLVFARSDALAEEFGLSETIQ
                     AFERGTRGTSYPMMAFSAGEPAKLWLDKRLQRFPHLGPEVRRRFLADPTEEFEVGEWL
                     AVTVSFNRQDYPFRCLVLGDLGPIVKRGQQRRINAARVSLTWVLLGIFLMLGPWRVRW
                     IYDSIKVRLGGAFLLAMLLPILGLGWTGASYLESEKSRLLREVRDRCRERIRKLELRY
                     QAFQSRQEVLWQDRMTALVSHADLKLPEIQAGLASMAAEGLFTHYYISEPDGRVALTN
                     YEIQDDSDRRSMQGISLMLKMIMTGRHAQEGDRRNGPPEGKELLQEEVRENIVQTLGT
                     KSNPQKFLRAGRLYATSLGNLNGFSMSLFFTYEGATRLIWVWATIGNVERAFARDEIQ
                     RWKLDTTGDRPLPYRLVFVRPGEYATHDVPATMAIDVNVLVAPRR"
BASE COUNT          258 a          354 c          405 g          295 t
ORIGIN      
        1 ctctcttctg ggacattgcc tcgcccagct acaatcagcg cacctatgtg ctcacgggtt
       61 tcgaatcgat gggcctcgtg ttcgcccgtt ccgacgcctt ggccgaggag ttcgggctat
      121 ccgagacgat tcaggctttc gagcgcggaa cccgaggaac cagttatccg atgatggctt
      181 tttccgcggg agaaccagcg aagctgtggc tcgacaagcg cctgcagaga ttcccgcacc
      241 tcggccctga ggtgcgacgc cgatttctcg ctgatccaac cgaagagttc gaggtgggag
      301 agtggttggc cgtcacggtg tcattcaacc gtcaggatta cccctttcga tgcctggtcc
      361 tgggagatct cgggcccatc gtcaagcgtg ggcagcagcg tcgtatcaac gccgcgcgtg
      421 tcagtctgac ctgggttctg ctcggcattt tccttatgct tggtccatgg cgggtgagat
      481 ggatctatga ttccatcaag gttcgtctcg gtggggcgtt tctgctggcg atgctgttgc
      541 cgattctggg ccttggttgg acgggagcca gttacttgga gtcggagaaa agtcggctgc
      601 tgcgagaagt gcgcgaccgt tgccgcgaac gcatccgaaa actcgaactg cgctaccagg
      661 cgtttcagag ccgccaggaa gttctttggc aggatcgcat gacggcactc gtcagtcatg
      721 cggacctcaa actgccggag attcaagccg gtcttgcttc gatggccgct gaaggattgt
      781 ttacccacta ctacatcagc gaacccgatg gtcgggttgc tctgaccaac tatgagattc
      841 aagatgattc agatcgtcgc agtatgcagg gaatcagcct gatgctgaag atgatcatga
      901 cggggcgtca tgcgcaggag ggggatcggc ggaacggacc accggagggc aaagagctgc
      961 tgcaggaaga agtgcgcgag aacattgttc agacgctcgg aaccaagtcg aatccccaga
     1021 agttcctgcg agctgggcgt ctgtatgcga ccagtctcgg caatctgaat ggcttctcga
     1081 tgagcctctt tttcacctac gagggtgcaa ccagattgat ctgggtgtgg gcaacgatcg
     1141 gaaatgtcga gcgggccttt gctcgcgatg agattcagcg ctggaaactc gatacgacag
     1201 gcgatcgacc gcttccctat cgtcttgtct ttgttcgtcc aggggaatat gccacccacg
     1261 acgtgccagc gacgatggcc atcgacgtga atgtgttggt tgcgccgcgc cg
//
LOCUS       DAORHD010001522         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145467_length_1311_cov_3.534236, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001522 DAORHD010000000
VERSION     DAORHD010001522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145467_length_1311_cov_3.534236"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PKO06_23150"
     CDS             complement(<1..928)
                     /locus_tag="PKO06_23150"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HNV72625.1"
                     /translation="MVNGPNCIFVEKQGRMTATTKTFTNNDHVKTVIDRILMSAGRTV
                     NERTPYVDARLADGSRVHAIIPPLSLTGPMITIRRFSHEPYTLEDLVYRFKTLTPEAG
                     EFLKMCVKFRKNIIVSGGASSGKTTLLNVLSSVIPQTERLIVIEDSAELRLTHDNMGR
                     LESRQQGTEAKNQITIRDLLRNALRMRPDRIIIGECRGDEALDMLQAMNTGHDGSLTT
                     LHANTTRDAIARLETMVLMAGVDLPLRAIREQIASAVSIIVQMTRFSDGTRRCIEIAE
                     VTGIEDTTLLTQPIFQFEKRWINENGAVFGDIV"
     gene            845..>1311
                     /locus_tag="PKO06_23155"
     CDS             845..>1311
                     /locus_tag="PKO06_23155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72626.1"
                     /translation="MVVIGEGFRGGGHAALLFDEDTVRAVDHDLADFGITHELFDRTE
                     AENLVEQKSARFGDDVNSAQERRRIAFHRILDAFFDATSQFGITQGVPLFHFGNDDPA
                     KLTLRANVPEAERAQARKLLAGYYAHCTALDDCFGELRRTLRETGLEETTLLV"
BASE COUNT          246 a          376 c          412 g          277 t
ORIGIN      
        1 ggacaatgtc cccgaagacg gcaccgtttt cgttgatcca ccgcttttcg aactggaaga
       61 tcggctgtgt gagcagggtg gtgtcttcga tgccggtgac ctcggcaatt tcgatacagc
      121 ggcgcgtgcc atccgagaag cgggtcatct gcacgatgat actcaccgcg ctcgcgatct
      181 gttctcgaat ggcgcgcagc ggcagatcga ccccggccat caggaccatg gtttcgagtc
      241 gggcgatcgc atcgcgcgtg gtgttggcat gcagcgtggt cagcgagccg tcatggccgg
      301 tattcatggc ctgcagcatg tcgagagcct catcgccgcg gcactcgccg atgatgatgc
      361 ggtcggggcg catgcgcagt gcgttgcgca gaaggtcgcg aatcgtgatc tgattcttgg
      421 cttctgttcc ctgctggcgg ctttcgagac gtcccatgtt gtcgtgcgtc aggcgcagtt
      481 cagccgagtc ttcgatcacg atcaaacgct cggtttgcgg aatgacggat gaaagcacgt
      541 tcaacaacgt ggttttgccc gaactggcac cgccggagac aatgatgttc ttgcggaact
      601 tgacgcacat cttgaggaac tcgcccgcct cgggagtcaa cgtcttgaaa cggtagacga
      661 ggtcttcaag ggtgtacggt tcgtgcgaga agcggcgaat cgtgatcatc ggcccggtca
      721 gggaaagcgg cggaataatc gcgtgaacac gggacccatc agccaatcgc gcatcgacat
      781 aaggagtgcg ctcgttcacc gtgcgacccg ccgacatgag aatgcggtcg atgaccgttt
      841 tcacatggtc gttattggtg aaggttttcg tggtggcggt catgcggccc tgcttttcga
      901 cgaagataca gttcgggccg ttgaccatga tctcgccgac ttcgggatca cgcatgaact
      961 cttcgatcgg accgaggccg agaatctcgt cgagcagaaa tcggcgcgtt tcggcgatga
     1021 cgtcaactcc gctcaggaga ggcggaggat tgcgtttcat cgcattctcg acgctttctt
     1081 cgacgcgacg tcgcagttcg gcatcactca gggcgtaccc cttttccatt tcggaaacga
     1141 cgaccccgcg aagctgacgc tgcgggcgaa cgtgcccgag gccgagcgcg cccaggcccg
     1201 caagctcctc gccggctact atgcgcattg cacggccctg gacgattgct tcggcgaact
     1261 gcgccgcacc ctgcgcgaga ccgggctgga ggaaaccacg ttgctggtgt t
//
LOCUS       DAORHD010001523         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145750_length_1310_cov_2.337052, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001523 DAORHD010000000
VERSION     DAORHD010001523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145750_length_1310_cov_2.337052"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKO06_23160"
     CDS             complement(<1..657)
                     /locus_tag="PKO06_23160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like FloA family protein"
                     /protein_id="HNV72627.1"
                     /translation="MEGMIVLIIIGGLIGFFTLSYFIPFGLWVSALASGVSVGMLDLI
                     GMRLRGVPQHLIINPAVMAKKGGLEVFLQALESHFLAGGNVSRVVSALIAAQRAGIPL
                     DFKRATAIDLAGRDVLEAVKMCVRPKVIATPEVAAMAKDGIQVKVIARVTVKADISKL
                     IGGATEETIIARVGEGIVTTIGSADTHKEILENPDRISKTIQEKGLDAKTAFEILSID
                     I"
     gene            complement(875..>1310)
                     /locus_tag="PKO06_23165"
     CDS             complement(875..>1310)
                     /locus_tag="PKO06_23165"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV72628.1"
                     /translation="GFGLTGIAGIIVIMSGLGLSFWKLDLRLAVIYTILSILALIGLV
                     FWALYVFPHTATGKKFVLETRISVEDGYTTTLELQKYVGQEGVAASDLRPSGIARIGD
                     ERLDVVSDGDFIPRGTRIKVIQAKQTNLVVAKLPEPESPPKA"
BASE COUNT          283 a          371 c          406 g          250 t
ORIGIN      
        1 aatgtcgatc gagagaatct cgaacgcggt cttggcgtcg aggccctttt cctgaatggt
       61 tttcgagatg cgatcggggt tctcgagaat ctctttgtgt gtgtcggccg aaccgatcgt
      121 ggtgacgatg ccttcaccga cgcgggcgat gatggtttct tccgtggcac caccgatgag
      181 tttcgagatg tcggctttca cggtcacgcg ggcgatgacc ttgacctgaa taccatcttt
      241 cgccatggcg gcgacttcgg gcgtcgcaat gaccttcggg cgcacgcaca ttttgacggc
      301 ctcgagcacg tcacgaccgg ccagatcgat ggcggtggcc cgcttgaaat caagaggaat
      361 accggcgcgc tgggcggcga tgagagccga tacgacgcgc gagacgttgc cgccggcgag
      421 aaagtgcgat tcgagcgcct gcaggaacac ctcaagaccg cctttcttgg ccatgacggc
      481 cgggttgatg atcaggtgct gcggtacgcc gcgaaggcgc atgccgataa gatcgagcat
      541 gccgacgctg acaccggaag ccagagcgct gacccagagg ccgaacggaa tgaagtaact
      601 gagcgtgaag aagccgatca gacctccgat gatgatcaat acgatcatgc cttccatgtg
      661 cagaccctcc tgagacggaa ttgattcgtg cgacaacggt tcagtacacc cggtactatc
      721 ccggaaatcc cgggcaaagt caagctgtgg ttcccgcgac cgtcagcggt tcccgcacgg
      781 atagacgatc acagctggcc ttcagaatct cacggacaca ttccaaccca cgagagctcc
      841 accgacgagg cagacgggaa aaagtcgaag gaggtcaggc ctttggcggg gattcgggct
      901 caggcagttt ggccacgacc agattggtct gtttggcctg aatgactttg atcctggtgc
      961 cgcgtggaat gaagtcgccg tctgagacga cgtcgagccg ctcgtctccg atgcgggcga
     1021 tgccggaggg gcgcaagtcg ctggcggcga caccctcctg accgacgtat ttctgcagtt
     1081 cgagcgtggt ggtatagcca tcctcaacac tgatgcgggt ttccagcacg aactttttcc
     1141 cggtggctgt gtgcggaaac acgtagagcg cccagaagac cagaccgatc agcgccagaa
     1201 tcgacagaat ggtgtagatc acggccagcc gcagatcgag tttccagaag gagaggccca
     1261 gtccggacat gatgacaatg atcccggcga ttcccgtgag gccgaacccc
//
LOCUS       DAORHD010001524         1309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_145844_length_1309_cov_3.744817, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001524 DAORHD010000000
VERSION     DAORHD010001524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145844_length_1309_cov_3.744817"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="PKO06_23170"
     CDS             <1..554
                     /locus_tag="PKO06_23170"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV72629.1"
                     /translation="FGAVLDKDTRDNRFRPSGGIHDTLSVETTGGMLRGPNQYTKYVL
                     ALRRYMPIRKGGETVLALQGVAGRTMIGEGFVPVYDMFSVGGNDTVRGYREREFLGTK
                     ILYGNIEIRQRIAKTFDIVAFYDMGDAWGLDYNQTSKDFDLKKGYGLGIRLQTPLGPV
                     AIDHGISPDRGEGRTYFNFGSSF"
     gene            615..1235
                     /locus_tag="PKO06_23175"
     CDS             615..1235
                     /locus_tag="PKO06_23175"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV72630.1"
                     /translation="MKKMMVSVALVCALFIAGPAWAFNFGLVDASKVFAKFNETQKTK
                     SLLEGEKTKMQQQLDAKKKDVADADAKYLELAKKIQTLRDQKKDAKAKAMEAQLKAQR
                     EALANANSELQKFFEESQKRLYDLEEEKMGNLSKALDEKVDAVIKKIAQAKGLEAVFE
                     KRMCYFPGDDDAKKIDITDDVIAALNTGAAAAPAAAPAAPAKKGGK"
BASE COUNT          322 a          331 c          401 g          255 t
ORIGIN      
        1 gcttcggtgc cgtgctggac aaagacacac gcgacaaccg gttccgcccc agtggtggaa
       61 ttcatgacac gctgtcggtc gagaccacgg gcggaatgct gcgcggaccg aaccagtaca
      121 cgaagtatgt gctggccctg cgacggtata tgccgattcg caagggtggt gagaccgttc
      181 tggcgcttca gggggtggcc ggtcgcacca tgattggtga aggctttgtt ccggtctatg
      241 acatgttcag cgtcggtggc aacgacacgg tgcgtgggta tcgcgagcgt gaattccttg
      301 gaacgaagat tctgtatggc aacatcgaaa ttcgccaacg catcgccaaa accttcgata
      361 tcgtggcatt ttatgacatg ggcgatgcct gggggctcga ctataaccag acttcgaaag
      421 acttcgatct gaaaaaagga tacgggcttg gcattcgcct tcagactcca ctggggccgg
      481 tggctatcga tcatggtatc tcccctgacc gcggtgaagg ccgaacctac ttcaatttcg
      541 gtagttcgtt ctaagagacg aacaccgttt acgcaggtta catacataca tccgttcgaa
      601 ggagagcaga cagcatgaag aagatgatgg tgagtgtggc gttggtttgt gccctgttta
      661 tcgccggacc ggcgtgggcg ttcaacttcg ggttggtcga tgcatcgaag gtttttgcca
      721 agttcaatga gacccagaag acgaagagtc tcctcgaggg ggaaaagacc aagatgcagc
      781 agcagcttga tgccaagaag aaagacgtcg cggatgcgga cgccaaatac ctcgaactgg
      841 ccaagaagat tcagaccctg cgcgaccaga agaaagacgc caaagccaag gccatggagg
      901 cccagctgaa ggctcagcgc gaagcattgg ccaatgccaa ttccgaactg cagaaattct
      961 tcgaggaatc ccagaagaga ttgtacgatc tggaagaaga gaagatgggc aatctgtcta
     1021 aggctctcga tgagaaagtc gatgccgtga tcaagaagat cgcccaggcc aagggtctcg
     1081 aagcggtgtt tgaaaagcgc atgtgctact tcccgggcga tgacgatgcg aagaagatcg
     1141 acatcaccga cgacgtcatc gcggcactca acacgggtgc tgctgcggct ccggccgccg
     1201 ctcctgctgc cccggccaaa aagggtggca agtaagtcgg aggatttgct acatcagcag
     1261 ggggcggcgg ccggagccgc agcagcaccc gtgttgagtg ccgcgatga
//
LOCUS       DAORHD010001525         1308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_146101_length_1308_cov_2.717478, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001525 DAORHD010000000
VERSION     DAORHD010001525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146101_length_1308_cov_2.717478"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKO06_23180"
     CDS             <1..425
                     /locus_tag="PKO06_23180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72631.1"
                     /translation="EGEHVELTFIGRSHPDAADFWDGNWLGTRVTARAGGFRAAFEAD
                     LRAEEIGAFHEALVAMNRSLSGDAEFLTLEQQVALTLSMNHTGQIAVSGTLTDQPGVG
                     NQLLLSFEIDQTYLHDIITRLECILEAFPQIGTPDAEA"
     gene            422..1240
                     /locus_tag="PKO06_23185"
     CDS             422..1240
                     /locus_tag="PKO06_23185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV72632.1"
                     /translation="MTDRCGGYAEHAFIAGLYDHLPAYRNRPDVTFFVEEAVACGGPV
                     LELGCGTGRVLIPTARRGVPVVGLDLSPVMLNTCRQFLRQEPEEVQARVELQEGDLRS
                     FNLGRTFPLITTPFRPFQHLLTVEDQIACLQAMRRHLTPEGRLILDLFNPSIYYLAHQ
                     GHTVEFGDDPEFALPDGSKVRRKFRIPRKDLHQQINDCESIFYVTHPDGSQERLVHTF
                     QIRYLYRFEVEHLLVRCGFKLLHVWCDYQRTPYGARYPGEMIFVATPDATEPAE"
BASE COUNT          245 a          356 c          401 g          306 t
ORIGIN      
        1 ccgaaggcga gcatgtcgaa ctgacgttca tcggccgctc acacccggat gccgcggatt
       61 tctgggatgg aaactggttg ggtacacggg tcacggcgcg ggcgggcggg tttcgagcgg
      121 cgtttgaggc cgatctgcgg gccgaagaaa ttggcgcttt tcatgaagcg ctggtcgcga
      181 tgaatcgctc actgagtggt gatgccgagt ttctcaccct ggaacaacag gtcgcgttaa
      241 ccctcagtat gaatcacacc ggtcagattg ccgtctcggg aaccctcacc gatcaaccgg
      301 gggtcggaaa ccaactcctg ctgtcgtttg agattgacca gacctatctg cacgatatca
      361 ttactcggct cgaatgcatt ttggaggctt ttccacagat cggcaccccg gacgcagagg
      421 catgaccgac cggtgtggcg ggtatgccga acatgcgttt attgcaggtt tgtacgacca
      481 tctgcctgcc taccgcaatc gccccgatgt gacctttttc gtcgaagagg cggtcgcatg
      541 tggcggtccg gtgctcgaac tcgggtgcgg aaccgggcgt gttctgattc cgactgcgcg
      601 acgtggagta ccggttgtcg gtctcgatct ttcgcccgtg atgctgaaca catgtcggca
      661 gttcctgcgg caagaaccgg aggaggtgca ggcgcgcgtc gagctgcagg aaggagactt
      721 gcgatcgttc aatctggggc gcacgtttcc gctgatcacg accccgtttc gtccgtttca
      781 acatctgctg accgtggaag accagattgc ctgcctgcag gcgatgcgac gtcatttgac
      841 ccccgaagga cgattgatcc tcgatctgtt caatccgtcg atctactatc tggctcatca
      901 ggggcatacg gtcgagttcg gcgatgatcc cgagtttgct ctgccagatg gttcgaaggt
      961 ccggcgcaag tttcgcattc cgcgtaaaga tctgcaccag cagatcaatg attgtgagag
     1021 catcttttat gtcactcacc cggacggtag tcaggaacgg ctggttcata cgttccagat
     1081 ccgttacctg taccggttcg aggttgagca tcttctggtt cggtgcgggt tcaagttatt
     1141 gcatgtctgg tgcgactacc aacgcactcc ctatggtgcc aggtatcccg gggagatgat
     1201 tttcgtggcg acgccagatg ccaccgagcc ggctgagtga acagggtctg gcatcgacga
     1261 tggcctcagg ccatgccgtg tcgatggtgg atcggcgtgc tgttgctg
//
LOCUS       DAORHD010001526         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_146276_length_1307_cov_2.859425, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001526 DAORHD010000000
VERSION     DAORHD010001526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146276_length_1307_cov_2.859425"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="PKO06_23190"
     CDS             complement(<1..490)
                     /locus_tag="PKO06_23190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV72633.1"
                     /translation="MWEEIFRARAWGKYPPEELIRFTARAFYRAPDRSSVKILDAGCG
                     PGACCWYLAREGFTVTGVDGSLCALQQARERLAQERLTGTWVRGDLLHLPFPEASFDG
                     VIDIAAVQQNRPAAIRSIIAGVSRILKPGGRFFSMMLAHGSWGDDLGRQIVTQMFISP
                     RVL"
     gene            complement(529..>1307)
                     /locus_tag="PKO06_23195"
     CDS             complement(529..>1307)
                     /locus_tag="PKO06_23195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNV72634.1"
                     /translation="QSLLEAMSGLPRLMAGIIYGTGMRLMECLRLRVKDISFEDGHIL
                     VREGKGRKDRVVPLPRTFAAELADHLAAVRRQHQQDLESGLGEVDLPNALARKFPAAG
                     KDWQWQYVFPSRHVSVDPRSQKIRRHHYHENSLQKAVKAAVREVGLPTTTSVHTLRHS
                     FATHLLEMGYDIRTVQELLGHADVSTTMIYTHVLKRPGVVIISPADMQGSPETGTVPV
                     LPPMVVKDRQTSAGNKDKTRAKKAHPAKTNHLPVKSSSRK"
BASE COUNT          245 a          377 c          410 g          275 t
ORIGIN      
        1 ccagcacccg cggcgagatg aacatctgcg tcacgatctg gcgtccgagg tcgtcgcccc
       61 acgatccgtg agccagcatc atcgaaaaga agcggccccc gggcttgaga atccgagaga
      121 cgccggcaat gatcgagcga atcgctgccg gtcggttctg ctgcacggct gcgatatcga
      181 tgacgccatc gaatgatgct tccggaaacg gcaggtgcag caggtctccg cgcacccagg
      241 tgccggtgag ccgctcttgt gcaagtcgtt cccgcgcctg ctgtaacgcg cacagagagc
      301 cgtcgacgcc cgtcacggtg aacccttcgc gggcgagata ccagcaacag gctcctgggc
      361 cacagcccgc atcgagaatt ttgacggaac ttcgatcggg tgcccgatag aacgcgcgag
      421 ccgtgaaccg aatcagttct tccggaggat acttccccca cgcccgggcg cggaaaattt
      481 cttcccacac cggatcccag ctgctgtttg tgagtgagtt cgagcgactc actttcgcga
      541 actcgacttt acgggaaggt ggttggtctt ggccggatgg gcttttttgg cgcgcgtctt
      601 gtctttgttg ccggcgctcg tctgacggtc tttgacgacc atgggcggaa gcaccggaac
      661 ggtacccgtc tcaggactgc cctgcatgtc ggccggactg atgatgacaa cgccggggcg
      721 tttgagcaca tgggtgtaga tcatggtggt gcttacgtca gcatgtccaa gcagctcctg
      781 aacggttcgg atatcgtatc ccatttcgag caggtgggtg gcgaaggagt gtcgcagggt
      841 gtgcacgctg gttgtggttg gcaggccgac ttcccgaaca gcggctttga ccgccttctg
      901 cagcgagttt tcgtgatagt ggtgtcgtcg gatcttctgt gaacgcggat cgacggaaac
      961 atggcgtgaa ggaaagacat actgccactg ccaatctttt ccggcggcgg gaaattttcg
     1021 ggctaaagca ttcgggagat cgacttcgcc gagaccgctc tcgaggtctt gctggtgttg
     1081 gcgccggact gctgcgaggt ggtcagccaa ctcggcggca aaggttcggg gcagaggaac
     1141 aactcggtct ttgcggccct tgccttcgcg cacgagaatg tgtccgtctt cgaagctgat
     1201 gtctttcacg cgcaggcgca aacactccat gagtcgcatt cccgttccgt agatgatgcc
     1261 agccatcagg cgcggcaacc cgctcatggc ttcgagcaag ctctgca
//
LOCUS       DAORHD010001527         1306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_146441_length_1306_cov_2.952038, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001527 DAORHD010000000
VERSION     DAORHD010001527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146441_length_1306_cov_2.952038"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKO06_23200"
     CDS             <1..319
                     /locus_tag="PKO06_23200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNV72635.1"
                     /translation="LYEAAAVDGAEDWHCFRHIILPQMKPMILFVLVINSIRTWQVFP
                     EVFILTRGSPLGTTDTIVHRLYETAFRFHDLGYASAMAWTLTLLLLLLSWLKTRALRD
                     SDR"
     gene            316..1146
                     /locus_tag="PKO06_23205"
     CDS             316..1146
                     /locus_tag="PKO06_23205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNV72636.1"
                     /translation="MSRLTTAPRPTIGRTLLLTAFTLVWLAPLFWMVVRSLSTTGMDG
                     LSSPSSLSFEQYRLVLFNSGFPRYIAKSVLVLLIVVGGNLVSAVLTGYAFARYRFPWR
                     DQLFGLILLTLMLPKQIIAVPILDVMVRLGLHDTLWALSLPFCVDGFNIFLMRQYLRR
                     MPTELEDAARIDGATEPAILRHVIVPICRPALALIIINTAIVNWNAFFFPLVLTDSAA
                     MRTLPVGLAVLTQGPHATDWGALIAGATISSVPMILLFLLFQREIIEGLTAGALSDQD
                     "
BASE COUNT          217 a          436 c          329 g          324 t
ORIGIN      
        1 gttgtatgaa gcagcggcgg ttgacggcgc tgaggactgg cactgctttc gccacatcat
       61 cctgccgcag atgaaaccga tgattctgtt cgtgctggtc atcaattcca ttcgaacctg
      121 gcaggtgttt cccgaggttt tcattctgac ccgcggaagt cccctcggaa ccaccgacac
      181 tatcgtccat cgcctgtacg aaaccgcctt tcgctttcac gatctcgggt atgcttcggc
      241 catggcctgg acgctcaccc ttctgcttct gctgctttcc tggctgaaaa cccgagccct
      301 gcgggactcc gaccgatgag ccgcctgacg acagcccctc gcccgaccat tgggcgcact
      361 cttctgctga cggcgttcac cctcgtctgg ctggctcccc tcttctggat ggtggttcgg
      421 tccttgtcga caaccggaat ggacggactc tcgtcgccgt cgtccctctc attcgaacag
      481 taccggctgg ttctctttaa cagcggcttc ccacgatata ttgcgaaaag cgttctggtt
      541 cttctcatcg ttgtcggcgg caatctggtg tccgccgtac tcaccggtta tgccttcgcc
      601 cggtatcgct ttccatggcg cgatcagctg ttcggactga ttctgctcac cctgatgctc
      661 ccgaaacaaa tcatcgcggt cccgatcctc gacgtcatgg ttcggctcgg cctgcacgac
      721 acgctctggg cattgtctct gccgttctgt gtcgatgggt tcaatatctt tctgatgcgc
      781 cagtatctgc gccgcatgcc gacggaactc gaagacgcgg ctcgcatcga cggtgcaacg
      841 gaacccgcga tcctgcggca tgtgatcgtt ccgatctgtc gtccggccct cgcgctcatt
      901 atcatcaata ccgcgatcgt caactggaac gcatttttct ttcctctcgt gctcacggat
      961 tctgcggcca tgcgaactct gccggtaggt ctcgccgttc tcacccaggg accgcacgcg
     1021 accgactggg gcgcactcat agccggagcc accatcagtt ccgttcccat gattctgctg
     1081 tttctcctgt ttcagcgcga gatcatcgaa gggctgaccg cgggcgctct cagcgaccag
     1141 gactgatccg cctcagcgcc gtctcctcct tccttctcga cccacgtaat gacgcaacag
     1201 ctttgccgtt cagacaactt cacggccctt gcggcatcct tcacggagca tcgatcttcg
     1261 ataacctgcc tcacgaggaa caacctgtcg atagctttgg gactca
//
LOCUS       DAORHD010001528         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_147661_length_1300_cov_2.542972, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001528 DAORHD010000000
VERSION     DAORHD010001528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147661_length_1300_cov_2.542972"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PKO06_23210"
     CDS             <1..552
                     /locus_tag="PKO06_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72637.1"
                     /translation="ELTAKITANIKAGNRAAAGQLALQLQTVKQQLQENSEQLQMSDK
                     TFRDLEKSRDVTVREAQAKIEKLQNMITETEMLNAQAELQEMAKGMISSIGTSGDTLN
                     RAQEIILENRDKAAGKARVASSQGLDTTEIVMKENEQAALGDQALAEFAAAYGIDMNS
                     GETASQPGQVVAPPVTEREMGPK"
     gene            598..>1300
                     /locus_tag="PKO06_23215"
     CDS             598..>1300
                     /locus_tag="PKO06_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72638.1"
                     /translation="MRMVRFALASLMLIGMLISPLYSQEAPVPPTTAVSGGSANANAA
                     EAPDANQVTTVKEYKFVAQEKLPPVQGTSNYKWDKKDKVVVFPINVWIGWLPIVAANH
                     GFRPSEESVFFKKFGFKVDLKLIDDPVAARDSFAAGNSHVLWGTLDMIALFAGELMKD
                     SRTAPRVVQQIDWSNGGDGIVVRTAIRSVTDLKGKTVAFAQKSPSQYFISNLLINAGM
                     RISDIKPKYTATAFEA"
BASE COUNT          322 a          377 c          371 g          230 t
ORIGIN      
        1 gagttgaccg ccaagatcac cgccaacatc aaggcgggca atcgcgcggc cgccggtcag
       61 ctggctctgc agttgcagac agtgaaacag cagttgcagg aaaattccga acagctgcag
      121 atgtccgaca agacgttcag agacctcgag aagtctcgcg acgtcaccgt tcgcgaggcc
      181 caggccaaga tcgaaaaact gcagaacatg atcaccgaaa ccgaaatgct gaacgctcag
      241 gccgagcttc aggaaatggc gaaaggcatg atctcctcga tcggcacgtc aggcgacacc
      301 ctgaaccgcg ctcaggagat cattctggaa aatcgcgaca aagccgccgg caaggcacgg
      361 gtggcctcga gccagggtct cgatacgacc gagatcgtca tgaaagaaaa cgagcaggcg
      421 gcgttgggcg atcaggcgct ggcggagttt gccgcggcct atggcatcga catgaattcc
      481 ggtgaaacgg cgagtcaacc cggacaagtg gtggcaccgc cggtcaccga acgcgaaatg
      541 ggtccgaaat aagaaagtac aagtcatttg tatactatca ctagaggaga agccaggatg
      601 agaatggttc gttttgctct tgcaagtctg atgctgatcg gcatgctgat cagcccgttg
      661 tacagtcagg aagctcccgt tccgccgacg accgccgttt cgggtggttc ggccaacgcc
      721 aatgccgccg aggcccccga tgccaaccag gtcacaaccg tcaaggaata caagttcgtt
      781 gcccaggaaa agctgccccc agtgcaggga acgtcgaact acaaatggga caaaaaagac
      841 aaagtggtgg ttttcccgat caacgtctgg atcggctggc tgcccatcgt tgccgccaac
      901 cacggctttc ggcccagcga agaaagcgtg ttcttcaaga agttcggctt caaggtcgac
      961 ctgaagctca tcgacgaccc ggtcgccgct cgcgattcgt ttgccgccgg caacagtcat
     1021 gtcctgtggg gcacgctcga catgatcgcg ctcttcgccg gagaactgat gaaagacagt
     1081 cgcaccgccc cccgtgtggt gcaacagatc gactggtcga acggtggtga cggcatcgtc
     1141 gttcgcacgg ccattcgctc ggtcactgac ctgaagggca agaccgtagc cttcgcgcag
     1201 aaaagcccat cgcagtattt catctcaaac ctgctgatca atgccggcat gcgcatttcc
     1261 gacatcaagc cgaaatacac ggccactgct tttgaagccg
//
LOCUS       DAORHD010001529         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_147796_length_1299_cov_3.377010, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001529 DAORHD010000000
VERSION     DAORHD010001529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147796_length_1299_cov_3.377010"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PKO06_23220"
     CDS             complement(<1..615)
                     /locus_tag="PKO06_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72639.1"
                     /translation="MSRPFNTRLLKAQLVISSLLILALVLGWLLLMEYRQTHLRAAMR
                     AWQPRFDEAVNLRDAGFPLGHHVWRFALDARRSFGDQERWREHIEDLQRMLGCRLEFQ
                     EFQHGVRVIVSPGTQASYTRELALLVEALRWSEAEGKARREDYERVSTRFFGRVCPLS
                     VLQRRNGEFQLAFPDGRPVLTFLERGRTGRGLAICLYDLDQKSMS"
     gene            complement(612..>1299)
                     /locus_tag="PKO06_23225"
     CDS             complement(612..>1299)
                     /locus_tag="PKO06_23225"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV72640.1"
                     /translation="VIEEYDLYGDCRFAGDLGGDILECRRLPDGRIFVMIGDVSGHGT
                     ASALLMAYVKAVVTLWIQDPDSRLEDLMLQLDRMLRADQVSGRFLAMFACVLDPRTHE
                     LAWVSGGHPFPLRRQADQTVTFLGHPCYPLGSRDQACQPQVRRFVVEPGESLWLYTDG
                     LVEALDATGRELGYQALADWVSRHGDAGTASERVKALQRLHDERVYKTDDDVTLLVLC
                     RHRRGPGGDA"
BASE COUNT          293 a          437 c          346 g          223 t
ORIGIN      
        1 gctcatcgat ttttggtcga gatcatagag acaaatagcc aacccgcgcc ctgtgcggcc
       61 tcgctcgaga aaggtgagta ccggtcgccc gtcggggaaa gccagttgaa actccccgtt
      121 ccgacgctgc agaaccgaca acgggcacac gcggccaaag aaacgggtcg atacccgttc
      181 gtagtcttcc cggcgtgcct ttccttccgc ttcgctccac cgcagcgctt cgaccagaag
      241 agccagttcg cgcgtatacg aggcctgcgt gccgggcgag acgatgaccc gtaccccgtg
      301 ctgaaactcc tgaaactcca gtcgacatcc cagcatgcgc tgcagatcct cgatgtgttc
      361 ccgccatcgt tcctggtcac cgaagctgcg tcgggcatcg agagcaaaac gccagacatg
      421 atgccccagc ggaaaccctg catcacgcag gttgaccgct tcatcgaaac gcggttgcca
      481 agcccgcatg gcagcccgca gatgcgtttg gcgatactcc atcagcagca gccacccaag
      541 cacgagggcc aggatcagca agctactgat gaccagttgc gctttcagca ggcgggtgtt
      601 gaacggccga ctcacgcgtc acccccaggc ccacgccggt gacgacaaag caccaacagg
      661 gttacatcat catctgtctt gtacacccgc tcatcatgca gtcgctgcaa tgctttgacc
      721 cgctcagaag ccgtaccggc atcaccatga cgagacaccc agtccgccag cgcctggtaa
      781 ccgagttccc gacccgtcgc atccagtgct tccaccaggc cgtcggtata gagccacaga
      841 ctctcacccg gctcgaccac aaaacgccgc acctggggct gacacgcctg atcgcgactt
      901 ccgagcggat agcacgggtg tccgaggaat gtcacggtct gatccgcttg ccgtcgcaat
      961 gggaacggat gaccacccga aacccaggcc aactcatgtg ttcgcggatc gagcacacac
     1021 gcaaacatgg ccaggaatcg cccactgacc tgatcggcgc gcagcatgcg atcgagctgc
     1081 agcatcaggt cttcgagtcg ggagtcgggg tcctgaatcc agagggtgac aaccgccttg
     1141 acatacgcca tgagcagcgc tgaggctgtt ccatgaccgc tgacatctcc gatcatcacg
     1201 aagattcgac catctggcag tcgacgacat tcgagaatat cgccgcccag atcgccggca
     1261 aaacgacagt ctccgtacaa gtcatactcc tcgatgacc
//
LOCUS       DAORHD010001530         1296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_148368_length_1296_cov_4.565673, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001530 DAORHD010000000
VERSION     DAORHD010001530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148368_length_1296_cov_4.565673"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..872
                     /locus_tag="PKO06_23230"
     CDS             246..872
                     /locus_tag="PKO06_23230"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV72641.1"
                     /translation="MKTITQGSLVAIHFTGKLENGEIFDSSAGNEPLKFKVGAGHVID
                     GLDEGIIGMRAGQVRDIKVTPEKGFGSYEDSSVLSVERAAMGKNMQPKPGMLIDIQTE
                     NGQRIPGTIVDVTEDTVKIDLNHPLAGKNLNFNVEVVEVHDEGDPGTENWGEHDHGGC
                     CGEHEDGCCGEHDHGCCGENEGEDDDEGCCGTHGKGGNGGKGGCCGGH"
     gene            complement(978..1199)
                     /locus_tag="PKO06_23235"
     CDS             complement(978..1199)
                     /locus_tag="PKO06_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72642.1"
                     /translation="MSFERVILNVCLAMLLAVCMLRLKGLVQFGRIRYRDRNKISTTA
                     INEGCLFASIIAATVFVCGLAGFIWIYWF"
BASE COUNT          310 a          345 c          409 g          232 t
ORIGIN      
        1 tatcaactgg cgtctcgatg tcggctcgaa ggaaggcggc atgcgctttc aaacgcagtt
       61 cgagattccc gtgttctggt gcacgcgccc cgaattgatc gaacatcgtg cgggaagcga
      121 ggggctgtga tacactcacc agaagtgttt gtaccgtttg ctcagaggga acaccgtgaa
      181 cgttcgcttt ctctgtgcgg tgggcgctaa cacagcatac cagtgggctt ggcccaggag
      241 tcatcatgaa aaccatcacg cagggaagtc tcgtcgccat tcactttacc ggcaagctcg
      301 aaaacggaga aatcttcgat tcaagtgcgg ggaatgaacc gctgaaattc aaggttggcg
      361 ctgggcacgt gatcgacggt ctcgacgaag gcatcatcgg tatgcgagcc ggtcaggttc
      421 gcgatatcaa ggtgacgccg gaaaaaggct tcggttctta cgaagactcg agtgtgttgt
      481 cggttgaacg cgccgccatg ggaaaaaaca tgcagccgaa gccgggaatg ctgatcgaca
      541 ttcagaccga gaatggtcag cgcattcctg ggaccatcgt cgatgtcacc gaagacacgg
      601 tgaagatcga cctgaaccac ccgttggccg gcaagaatct gaattttaac gtcgaggttg
      661 tcgaagtgca tgatgagggc gaccctggaa ccgaaaactg gggcgaacat gatcatggcg
      721 gctgctgcgg cgaacacgag gatggctgct gtggcgaaca tgaccacggg tgctgtggtg
      781 agaatgaagg cgaagacgat gacgaagggt gctgcggcac gcacggcaaa ggcggcaacg
      841 gcggaaaagg tggctgctgc ggcgggcact gatcgatcaa ccgcgccgcg cgcggcgaca
      901 gaatgtattg gcagaacagg ccgatccgtc agggtcggcc tgttctttca gggcggcgca
      961 gagcgaatga gaaccgctca gaaccaatag atccagatga acccggccag cccgcacaca
     1021 aaaaccgtgg cggcgatgat cgaggcaaac aggcagccct cattgatggc tgtggtcgag
     1081 atcttgttcc gatcacgata tcgaatgcgt ccaaactgga ccaacccttt cagacgcagc
     1141 atgcagacgg cgagcagcat ggcgaggcag acgttcagaa tcacccgctc aaaactcatg
     1201 gtcgacccga cgccagtcga ttacggcgtc cactgggcgg tcaggtgctg ctctgcgccg
     1261 agcgcggctg acgcgccgct gccgcacgcg acgatc
//
LOCUS       DAORHD010001531         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_148640_length_1295_cov_2.866129, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001531 DAORHD010000000
VERSION     DAORHD010001531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148640_length_1295_cov_2.866129"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="PKO06_23240"
     CDS             <1..401
                     /locus_tag="PKO06_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72643.1"
                     /translation="LVTKAKPAGVTFERLRVISQSEEGGMSAPQEEEDEEELAAGDAA
                     AGSDATNSPPPGAPPTSAAPVTSFGSDGVENLELRGRILEVKGTCTSLEDLVSFTGQL
                     RASGLVSRLRLVRSKPSGRSTRFTVRGDLP"
     gene            398..1069
                     /locus_tag="PKO06_23245"
     CDS             398..1069
                     /locus_tag="PKO06_23245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72644.1"
                     /translation="MKQKTFLYVFIALLAVSLFLIIGRQLIIAPLLEKTASVRQTSAV
                     QNTQIESLNKQLANLKKDGVDSPQSTRLLPAGSEHQILQTIVSAASTTGFIVEALVLQ
                     PSFQKRNKPGELAESGGTPTLKPGELPPVDENGNAVGMTTEDDDNGPSVEIVPVRVQG
                     KGALRAWGLFLRQIRQELPLFGFRELVMQYTSTEPKGSFEIVVPLKTAVQASSGLPQT
                     LTGGK"
     gene            1071..>1295
                     /locus_tag="PKO06_23250"
     CDS             1071..>1295
                     /locus_tag="PKO06_23250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72645.1"
                     /translation="MGNDTWKWALVLVLSAVFILVAHQAYKSLHNADDQFPPPDDYLK
                     GDSYDLEHGTVPGPVGSTPIDGVTTPAENPI"
BASE COUNT          302 a          380 c          366 g          247 t
ORIGIN      
        1 tcctcgtcac caaagccaaa cccgccggag ttacctttga aagactgcgt gtgatcagcc
       61 agtccgaaga gggcggcatg tccgctcccc aggaggaaga agacgaagag gagcttgccg
      121 caggcgatgc ggcggcagga agtgacgcaa cgaattcgcc accgccggga gcaccgccga
      181 cgtcagcggc ccccgtcact tcgttcggtt cggacggggt cgagaatctc gaactgcgcg
      241 gccgcattct ggaagtcaaa gggacatgca cgagcctcga agatctcgta tcctttaccg
      301 gccaactacg tgcctctggc cttgtcagca gattgcgact agtacgcagc aaaccatcag
      361 ggcgcagcac gcgattcacc gtgcgaggag atctgccatg aaacagaaga ccttcttgta
      421 tgtcttcatt gcgctgcttg ctgtctcgct gtttctgatc atcggtcggc agctgattat
      481 tgcgcccctg ctggaaaaga ccgcctcggt gcgccagacg agtgcggttc agaatactca
      541 gatcgagtcg ttgaacaaac aactggcgaa cctcaagaag gatggcgtgg attcacctca
      601 gtcgacccgg cttctgccag cgggttccga acatcagatt ctacagacga ttgtctcagc
      661 cgcatcgaca accggattca tcgtcgaagc cctggtactg caaccgtcat tccagaaacg
      721 caacaaacca ggagaactcg cggaaagtgg cggcactccg accctgaagc ccggcgaact
      781 gccccccgtc gacgaaaacg gtaatgctgt cggcatgaca actgaagatg acgacaacgg
      841 cccaagcgtt gagattgttc ccgtgcgcgt gcaaggaaag ggagccctga gagcatgggg
      901 attgttcctg cgacagattc gccaggaact gccgctcttc gggttccgtg aattggtgat
      961 gcagtatacg tcgaccgaac cgaaaggttc gtttgagatc gtggttccgc tcaagacggc
     1021 cgtccaggct tcgtcaggcc tgccacagac actgacagga ggaaaatgac atgggcaacg
     1081 acacctggaa atgggctttg gtgctggttt tatctgcagt gttcatcctg gtggcccacc
     1141 aggcgtacaa gtcactgcac aatgccgatg atcagtttcc ccctccggac gactatctga
     1201 agggagactc atacgatctc gaacacggca cggttcccgg accggtcggc agcacaccaa
     1261 tcgacggtgt caccactccc gccgagaatc cgatc
//
LOCUS       DAORHD010001532         1293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_149086_length_1293_cov_2.527464, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001532 DAORHD010000000
VERSION     DAORHD010001532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149086_length_1293_cov_2.527464"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="PKO06_23255"
     CDS             complement(<1..130)
                     /locus_tag="PKO06_23255"
                     /inference="COORDINATES: protein motif:HMM:NF014934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72646.1"
                     /translation="MNGKGAGNTLSRAEIDELGEVAQRYGAKGMAWVKVAADGWHGP"
     gene            158..>1293
                     /locus_tag="PKO06_23260"
     CDS             158..>1293
                     /locus_tag="PKO06_23260"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNV72647.1"
                     /translation="MTALHVVHLRPQVRDLLVKSLVSLGHSALAAAHALEIVHRDLKP
                     SNILIAADGQPKIVDFGLARTADSGDLSSTGHFLGTLRYASPEHFGRGRVPVTARSDL
                     FSLGLVFYEVATLRHPVTATDESGIIGQIVQGDFADPREWNPHLPAGIARAILKSLRP
                     APDDRFADATAMKEAIRLGEKAGSWVGGLKEFFFGLFQSSSRPQPSPTPVRDAPPTAT
                     TAVPDRTPTTPPARQLRRTPPIPPTPPAPLKRSAPSETPEPPSSSARKRPSMAQIPSL
                     LGEARDELLQRFAYGPAIALLEQILDLDPANVDALFLLAFAYSSIGEHQAVKTALLKL
                     EAQAGETLDERETLKLAIIREHYLDLEYSRCLSHALNLSRRWPD"
BASE COUNT          231 a          468 c          339 g          255 t
ORIGIN      
        1 ccgggccatg ccagccgtcg gccgccacct tcacccacgc catgcccttg gcgccgtagc
       61 gctgagcgac ctcgccgagc tcgtcgatct cggcgcgcga caaggtattg ccagcaccct
      121 tgccgttcat cgcttgctgc acaagcacca acgcatcgtg acggcccttc atgtagtaca
      181 tctgcgtccg caagtccgcg atcttctggt caagagcctt gtctcgcttg ggcactctgc
      241 ccttgccgcc gcacacgcac tcgagatcgt gcatcgcgat ctgaaaccct ccaacattct
      301 catcgccgcc gacggccaac ccaagatcgt cgatttcggg ttggcgcgca ccgctgactc
      361 aggtgatctg agcagtaccg gtcattttct cggcaccctg cgctacgcct cgcccgaaca
      421 cttcggccgg gggcgggttc ccgtcaccgc ccgctcggac ctgttcagtc tcggtctggt
      481 tttctacgag gtggcgaccc tgcgacaccc agtcaccgcg acagacgagt cgggaatcat
      541 cgggcagatc gtccagggtg atttcgccga tccgcgggaa tggaacccgc atctgccggc
      601 cggaatcgcc cgggcgattc tcaagagcct gcgtcctgcg cctgacgatc gttttgcaga
      661 cgccacggcc atgaaggaag ccattcggtt gggggaaaag gccggttcct gggttggcgg
      721 cctcaaagag ttcttcttcg gtctgttcca atcgtcttca cgaccacaac cctcgcccac
      781 tcccgtccgc gatgccccgc ctacggcgac aaccgcagta ccagaccgta cgccaaccac
      841 gccccctgcc cgccagctcc gtcgcacccc accgattcca ccgacaccac ccgcacccct
      901 gaaacggtcg gcaccgtctg aaacacctga acctccgagc tcgtccgcac gaaaacgtcc
      961 ttcgatggct cagataccat ctcttctcgg cgaagctcgc gacgaactgc tgcaacgttt
     1021 cgcgtatggt ccggctattg ccctgctcga gcagatcctc gatctggacc cggcgaacgt
     1081 ggacgctctg tttctgctgg cgttcgccta ctcttcgatc ggtgagcatc aggccgtcaa
     1141 gactgcgctg ctgaagctcg aagctcaggc cggcgagacc ctcgatgagc gagaaaccct
     1201 gaaactcgcc attattcgcg aacactatct cgatctcgag tattcccggt gtctgtctca
     1261 cgcgctcaat ctctccagac gctggcccga tga
//
LOCUS       DAORHD010001533         1292 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_149227_length_1292_cov_3.219078, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001533 DAORHD010000000
VERSION     DAORHD010001533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149227_length_1292_cov_3.219078"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PKO06_23265"
     CDS             complement(<1..1069)
                     /locus_tag="PKO06_23265"
                     /inference="COORDINATES: protein motif:HMM:NF017874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vps62-related protein"
                     /protein_id="HNV72648.1"
                     /translation="MSSHSRYCFLLVFFMTGASFAIAQAPSAFEDALAAIVDQSEKPQ
                     KPKTQPAIKSSAGSQKLSSSSQAHSAGLQPTDKYFKNVYDSINKILLRNAIVTPHFHC
                     LYTRSHSLVWRDKGTGGAFDIGIWRPQLNHGWVRLGDIAVPSMSEDCPSAPALIIYWD
                     DRNPAGDEKGPFLKKPIDYRWVWDSEKSGADRKGSIWYPVPPAGYVSLGLVAQNSWSK
                     PDDLGIVRCVRSDIVLPGKWIGTMWTDKKSGAKHDCAVWTQTVADTYAKKPFLVTGTV
                     HAVGSYDSPPSVPPYVLKGLSGEDAYNDARNGYLKLTRNGMLSRMQLAVANARATDQT
                     ELASEANDLQRKLGQVESVAPN"
BASE COUNT          293 a          315 c          368 g          316 t
ORIGIN      
        1 gattgggtgc gacactctcg acttgtccga gtttcctttg caggtcattg gcttcagatg
       61 ccagttctgt ctggtctgtt gcgcgggcgt tcgcgaccgc gagttgcatg cgcgaaagca
      121 tgccgttacg ggtaagtttc aggtagccgt tacgggcatc attgtaggca tcttctcctg
      181 aaaggccttt caaaacgtaa ggaggcacgg agggggggga gtcatagctt cctacggcat
      241 gaacggtacc agttaccaga aagggtttct tggcgtaggt gtcggcaacg gtctgcgtcc
      301 agaccgcgca atcgtgcttg gcacctgatt tcttgtcggt ccacatcgta ccgatccatt
      361 ttccggggag aacgatatct gatctgacac agcgaacgat gcccaggtcg tctggttttg
      421 accagctatt ctgagcgacg agtcccaacg agacatagcc ggctggcgga acgggatacc
      481 agatcgaccc cttccggtca gccccggact tttcgctgtc ccatacccat cgatagtcga
      541 tgggtttctt cagaaaagga cctttctcgt cacccgctgg attcctgtcg tcccagtaaa
      601 tgatgagtgc cggagcgctc ggacagtcct ctgacatgga aggaacggcg atgtcaccga
      661 gcctgaccca gccatggttc agttgagggc gccagatacc gatgtcaaag gctccgcctg
      721 ttcctttgtc tcgccagacc aatgaatgag agcgtgtgta cagacagtga aagtgaggtg
      781 tgacgatggc attcctgagc agtatcttgt tgatcgagtc gtagacattt ttgaagtatt
      841 tgtccgttgg ctgcaggcca gcagagtgcg cctgcgagct gctcgacagc ttttgactac
      901 ccgcagaaga cttgatggcc ggttgtgttt tcggcttttg aggtttctct gactggtcga
      961 caatggcggc cagagcatct tcaaaagcgc tcggagcctg cgctatggca aaactggcac
     1021 cggtcatgaa gaaaacgagc agaaagcaat atctcgaatg tgaggacacg caaatgaacc
     1081 tccgcaataa gttcggcttc agtcagatga ctgaacgttc gaaaagacag tatcgcacag
     1141 acgctcgagt tcgtcatgta catttgaaat ctggaatact caggttagga catgtgtacg
     1201 agtcagagtg agttctgttg ctttataaat gcgtgtgacg gcggcataac aatgcaagtg
     1261 tgtcgtgtcg tcgaaatgag aaaattcgtc ga
//
LOCUS       DAORHD010001534         1289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_149799_length_1289_cov_3.241491, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001534 DAORHD010000000
VERSION     DAORHD010001534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149799_length_1289_cov_3.241491"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..643)
                     /locus_tag="PKO06_23270"
     CDS             complement(44..643)
                     /locus_tag="PKO06_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72649.1"
                     /translation="MKRVSICLCVVLSLMLNLNAFGQQSDLNTTNRVYFQELTNLAFG
                     GYGDLDYWSKQSKLIAFRSAVHLTSVAISINADRDHGDRRMAKFKMIVELNKAMMGKA
                     KDFAFGGYGDTDYWSKRTKHFATEIAMHCSTVAGMSDKVMLKAIQPKIETLLRAAQTA
                     GFAGFGDVDYWSRRCKAFATQAIEILGEISTELATAVVQ"
     gene            complement(805..>1289)
                     /locus_tag="PKO06_23275"
     CDS             complement(805..>1289)
                     /locus_tag="PKO06_23275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV72650.1"
                     /translation="GRDVRGMGEEALTEIRRGRLGFVFQKFHLLPSLSVRENVELPLL
                     FLGRAPDQNFTDEVLGLVDLRDRAEHLPRELSGGQMQRVAIARALITRPPLLIADEPT
                     GNLDRVNGEAVFNLFRRLVREQGKTVIITTHNLALGYLADRVITLEDGRILREETGRH
                     "
BASE COUNT          258 a          351 c          396 g          284 t
ORIGIN      
        1 cccccgaagc cgtggcctcg ggggtgcgtg tcgaacgggg aagttactgg acgacggcgg
       61 tggcgagttc ggtgctgatc tcgccgagaa tttcgatggc ctgcgtggca aaggctttgc
      121 agcggcggct ccagtagtca acgtcgccga atccggcgaa accggcagtc tgggcggctc
      181 tgagaagggt ctcgatcttc ggctgtatgg ctttcagcat gaccttgtcg gacatgccgg
      241 caacggtcga gcagtgcatg gcaatctcgg tcgcgaagtg cttggtgcgc ttgctccaat
      301 aatcagtgtc gccgtatccg ccgaaggcga aatccttggc tttccccatc atggctttgt
      361 tgagttccac gatcatcttg aatttggcca tgcggcggtc tccatggtcg cggtctgcgt
      421 tgatggagat ggcgacagat gtcagatgca cagcggaacg aaaggcgatc agcttggact
      481 gcttcgacca atagtcgaga tcaccatacc cgccgaaagc gaggttcgtc agttcctgaa
      541 aatagacgcg gttggtggtg ttgagatcgg actgctgacc gaaggcattc aggttcagca
      601 tcagagacag gaccacacac aggcagatcg atactcgttt catcactcac tccttacaat
      661 acaacttcgt gtcgttgaac tgacaccagt gtatcagagg ctgtgagttg gttcaattcg
      721 gcatgatcgc cagggtcatt caatgctcgt cctggttttt cgttggcaga agcgaaactt
      781 gtgagccgtc cggtcagggc ggtctcaatg tcgccccgtc tcttcgcgca ggatccttcc
      841 gtcctcgagg gtaatcaccc ggtcggccag atagccgaga gcaaggttgt gggtcgtgat
      901 gatcacggtt tttccctgct cgcgaacgag tcgacggaag agattgaaca ccgcttcacc
      961 gtttacccgg tcgaggttcc cggtcggctc gtctgcgatc agcagaggcg gtcgcgtgat
     1021 caaggccctg gcgatggcca cacgctgcat ctggccgcct gacagctcgc gtggcagatg
     1081 ttcggcgcgg tctcgcaggt caacgagtcc caggacttca tctgtgaagt tctgatcagg
     1141 agcgcgcccc agaaagagaa gaggcagttc gacgttttcg cggaccgaca gggaggggag
     1201 aaggtgaaat ttctgaaata cgaatcccag gcgaccacgt cgaatctcgg tcagcgcttc
     1261 ttcacccatg ccgcgcacgt cgcgtccgg
//
LOCUS       DAORHD010001535         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150201_length_1287_cov_3.443182, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001535 DAORHD010000000
VERSION     DAORHD010001535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150201_length_1287_cov_3.443182"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKO06_23280"
     CDS             complement(<1..532)
                     /locus_tag="PKO06_23280"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNV72651.1"
                     /translation="MSTTYKPSLTPEQFNSLLHEGVLVFDGAMGTNLQRQNLTPEAFG
                     GLEGCSEALNLFCPQAPCKVHADFLRAGCRVVESNSFGATRLVLQEYGLENRVDEINR
                     AAIRIAREAIEEARQPHRCFVAASIGPSTKLPTLGHIGFAAMRDIFAEQVEAQLLGGA
                     DLLIIETCQDLLQIKAA"
     gene            complement(534..>1287)
                     /locus_tag="PKO06_23285"
     CDS             complement(534..>1287)
                     /locus_tag="PKO06_23285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNV72652.1"
                     /translation="INIPDSPRASARMSPLSLAILIEQQTGIETILHYCCRDRNILGI
                     QSDVLGAAAVGLRNILCITGDPPKVGNYPEATAVFDIDAIGLTNVVYRLNHGRDLGGN
                     PIGKPTGFFIGVGANPGAIDLDLEVRRFRWKVDAGAEFAITQPVFDTTLLERFLERIS
                     DIRIPLIAGIWPLASLKNAEFLNNEVPGASVPEPLMERMRAAKTLEQQREEGIRIAQE
                     ALLKLRPWVQGVQISVPFGRAEFVGRVLEVMK"
BASE COUNT          264 a          360 c          396 g          267 t
ORIGIN      
        1 gagctgcctt gatctgcagc agatcctggc aggtttcaat gatcaagaga tcggcaccgc
       61 ccagcagttg tgcctcgacc tgttcggcaa agatgtcgcg catggcggcg aatccgatgt
      121 gtccaagagt cggcagtttc gtgctcgggc cgatcgaggc cgcgacgaag cagcggtggg
      181 gctggcgagc ctcttcgatg gcctcgcggg cgatgcgaat cgcggctcgg ttgatctcat
      241 cgacacgatt ctcgagtccg tattcctgca agaccaggcg tgtcgcgccg aacgagttcg
      301 attcgaccac gcggcaaccg gcgcgcagaa aatcggcatg caccttgcac ggcgcttgag
      361 gacaaaagag attgagcgct tcgctgcatc cttccagacc accgaaggct tctggggtca
      421 gattttgccg ctgcagattg gtgcccatgg cgccgtcgaa cacgagaact ccctcgtgca
      481 atagactgtt gaactgctct ggagtgagtg atggcttgta cgttgtcgac atgctatttc
      541 atgacctcga gaacccggcc gacgaattcc gcccggccga acggcacgct gatctgcaca
      601 ccctgcaccc agggtcgaag cttcagcagc gcttcctgcg cgatgcgaat cccttcctcg
      661 cgttgctgtt cgagagtttt cgctgccctc atgcgctcca taagaggttc tggcacagat
      721 gcgcctggga cttcgttgtt gagaaactcg gcattcttga gactagccag tggccagatg
      781 ccggcaatga gcggaatgcg gatgtcgctg atacgttcga gaaaacgttc gagcagcgtc
      841 gtatcgaaaa ccggttgcgt gatggcgaat tcagctccgg catccacctt ccagcgaaag
      901 cggcgaacct cgagatcaag atcgatggca ccggggtttg ccccaacacc gatgaaaaag
      961 ccggttggct ttccgatggg gttgccgccg agatcacgcc cgtggttgag acgatagacg
     1021 acgtttgtga gaccgatcgc atcaatatcg aagacagccg tggcctcagg gtagttgccg
     1081 accttgggcg gatctccggt gatgcacaga atgttacgca agccgaccgc tgcggcaccg
     1141 agcacgtctg actgaatgcc aagaatgttg cggtcacggc agcagtagtg aaggatcgtc
     1201 tcgatgccgg tttgctgttc gatgagaatc gccagcgaca gtgggctcat gcgcgctgaa
     1261 gcccgtgggc tgtcgggaat gttgatg
//
LOCUS       DAORHD010001536         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150232_length_1287_cov_2.825487, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001536 DAORHD010000000
VERSION     DAORHD010001536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150232_length_1287_cov_2.825487"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKO06_23290"
     CDS             complement(<1..306)
                     /locus_tag="PKO06_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72653.1"
                     /translation="MNKRWLLIGCVFLWCATSTIVCAETTPSQLLLDAGIRHKLQIVA
                     VMPSAGAGDVKEPSASETSTVFDLAKTLGMQVQVKGDLAVFGEKMAGDPNRLGQTLTG
                     "
     gene            complement(380..>1287)
                     /locus_tag="PKO06_23295"
     CDS             complement(380..>1287)
                     /locus_tag="PKO06_23295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV72654.1"
                     /translation="HGAFHGRTFGAMSLTCSKAVQKRHYAPLLPMVDHVPYGYCYRCH
                     YGKTYPKCELHCISVIEKHYFKSQIPAEEVAAFIIEPIQGEGGYVVPPPDYHQNLKKL
                     AEKYGILYVVDEVQSGMGRTGKMFAIEHWGVEPDIVATAKGIASGLPLGAIIAKADVM
                     NAWEPGCHASTFGGNPVACAAANATVALLESELMDNARKMGEVIVKGLKALQKRLMCI
                     GDVRGMGLMIGVELVHDRRTNEPDPKLRDALVTEMYKQGIIVLGCGSHTLRLCPPLVV
                     NREQCDFVVETIGKCLEKLTAGRQP"
BASE COUNT          255 a          402 c          371 g          259 t
ORIGIN      
        1 gcccgtaagc gtctgcccca gacgattcgg gtcgcccgcc atcttctcgc cgaacacggc
       61 caggtcgcct ttgacctgca cctgcattcc gagagttttg gcgagatcga acaccgtgct
      121 cgtctcagaa gccgacggtt ccttgacatc acccgcgcca gcggaaggca tcacggcgac
      181 gatctgcagc ttgtgacgga ttccggcatc gagcagcagt tgagacggag tcgtctctgc
      241 acagacgatc gtactggttg cgcaccagag aaaaacgcac ccgatcaaga gccaacgttt
      301 gttcatacac attcctcccg gattagcaaa cggcccgacc tccggacgga agtcgggccg
      361 cagttgatac ctcgtctgat cagggctggc ggccggcagt cagcttttcg agacacttgc
      421 cgatcgtctc gacgacgaag tcgcactgct cgcgattgac gaccaacggc gggcacaggc
      481 gcaacgtatg gctgccacag cccagaacga tgattccctg cttgtacatt tcggtcacca
      541 gcgcatcgcg cagcttcgga tcgggctcat tggtgcgacg gtcgtgtacc agttcgacac
      601 cgatcatcag ccccatgccg cgcacgtctc cgatgcacat gagtcgtttc tgcagtgcct
      661 tgagcccctt cacgatcacc tcgcccatct tgcgggcgtt gtccatgagc tcactctcga
      721 gcagagcaac cgtcgcgttg gcggccgcgc aagccacggg attgccgccg aaggtgctcg
      781 catgacagcc gggttcccag gcgttcatga catcggcctt ggcgatgatc gcgcccagcg
      841 gcaaacctga ggcaatgccc ttggcggtag cgacgatatc aggctcgacg ccccagtgtt
      901 cgatggcaaa catcttgccg gttcgaccca tgcccgactg cacttcatcg acgacataca
      961 gaatgccgta tttctcggcc agcttcttga gattctgatg gtagtcggga gggggaacga
     1021 cgtatccgcc ttcgccctga atcggctcaa tgatgaacgc cgcgacttct tcggccggaa
     1081 tctgagactt gaagtaatgc ttctcgatga cgctgatgca gtgcagctcg catttcgggt
     1141 aggtcttgcc gtagtggcag cgataacagt agccgtatgg cacgtggtcg accatcggca
     1201 gcaacggcgc atagtgccgc ttctgcacgg ctttcgagca ggtcagggac atcgccccga
     1261 aggtgcggcc gtgaaatgcg ccgtgaa
//
LOCUS       DAORHD010001537         1287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150235_length_1287_cov_2.804383, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001537 DAORHD010000000
VERSION     DAORHD010001537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150235_length_1287_cov_2.804383"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1287
                     /locus_tag="PKO06_23300"
     CDS             17..>1287
                     /locus_tag="PKO06_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72655.1"
                     /translation="MATKISQSLYGSLVTLPDFTPTDDMPRLVQTKGVMKLSIFLPRE
                     ILVSLNASATSDLRATRNATNTGGFDGIWILLDPRLLSCREVEDRVLSFLSRLADQVF
                     IVQRVGGRPRIIRKEGQGGISQQILNRFFQGQTQSTDALGTWIARYIPDERGRYLIAG
                     MRPKRFQGFLDQAAPFIRAACLLLLVFGSRLAFLRLALGRPLAIRLSLLVFAMFALTT
                     VLPLAFVLSQGLSLAHEQARQAQIEWEYRLKEKLTAIDHRHRQFQARRQTVLHELEAR
                     LIEKLPVATGAASPLPEICAGLEKVAVLVHDENGKKLAWSRNLENLLDRGDYPEFQAR
                     YLARQISSEGRTPCADLTGGFLLVSGYALEAVINRLFNESVGFNRLRVRGRNFWQRQA
                     PLEKSTDGTVRTIGARQRERRRGLAIAVDGPG"
BASE COUNT          250 a          383 c          364 g          290 t
ORIGIN      
        1 cgataagcgc tttcacatgg ccaccaagat cagtcagtcc ttgtacggaa gcctggtcac
       61 tctgcccgac ttcacaccca cggatgacat gccccgacta gtacaaacca agggggtcat
      121 gaagctttcg atctttctgc cccgagagat tcttgtatca ctgaatgctt cagcaacctc
      181 agatcttcgc gcgacccgca atgcgacgaa taccgggggg ttcgacggca tctggatcct
      241 cctggaccca aggcttctga gctgtcgaga agttgaagat cgggttcttt cgttcctgtc
      301 caggctggcc gaccaggttt tcatcgtgca gagggtaggg gggcggcctc gaatcattcg
      361 caaagaaggc cagggcggta tctctcagca gatcttgaac cgcttcttcc aggggcagac
      421 gcagagtact gatgctctgg gcacctggat tgcccgctac attcccgacg agagaggtcg
      481 atatctcatt gccggaatgc gtccgaaacg tttccaggga tttctggatc aggcggctcc
      541 cttcattcgt gcggcttgtc ttctcttgtt ggttttcggc tcgcgacttg ccttcctccg
      601 actggccctg gggcgcccgc ttgcgatacg gttgtcgctt ttggtattcg ccatgtttgc
      661 gctgaccacc gtccttccgt tggcttttgt cctatctcaa ggactcagtc ttgctcatga
      721 acaggcccgc caagcacaga tcgaatggga atatcgcctg aaggaaaagc tgaccgccat
      781 tgatcaccgg catcgccagt ttcaggcccg acggcagacc gtccttcacg agcttgaagc
      841 ccggctgatt gagaagcttc ccgtcgccac cggggccgcc tcgccccttc cggaaatctg
      901 cgccggactg gaaaaggttg ccgtactcgt tcatgacgag aacgggaaga agcttgcctg
      961 gagcagaaac ctggagaatc tcctcgatcg aggagactat ccggaattcc aggctcgata
     1021 tctcgcccgc cagatttcgt cggaaggccg gacgccctgc gccgatctca ccgggggctt
     1081 tctgctggtt tcaggatacg cgctggaggc agtcatcaat cgtctgttca atgaatcggt
     1141 ggggttcaac cgcttgcgtg tacgaggtcg caacttctgg caacgacagg ccccgcttga
     1201 gaagtcgact gatggaaccg ttcgcaccat cggcgcacga cagcgcgagc gacgccgtgg
     1261 actcgccatc gccgtcgatg ggcctgg
//
LOCUS       DAORHD010001538         1286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150392_length_1286_cov_3.544273, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001538 DAORHD010000000
VERSION     DAORHD010001538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150392_length_1286_cov_3.544273"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKO06_23305"
     CDS             <1..690
                     /locus_tag="PKO06_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72656.1"
                     /translation="LALNGMKTLPGYRDAIRLVAKTVEMKTWPEAGLYYPQLLIFPYT
                     AARAWRDGGAREPEMQVAMGKLLLDLLDLRDRYAREKPRHRGAFPGGEDRSDHLSTAL
                     GLITLINIGQETAERMGVSERYTQALDEAVLYLIKIGDEAKPHNQTTEARLGRQTRPR
                     RWDSGLFFAASFWDLGHWRSQPFTVAMVLEALTKYALLHERSGKPFGAARLLIQPDPA
                     RSGRLVLAPSL"
     gene            771..>1286
                     /locus_tag="PKO06_23310"
     CDS             771..>1286
                     /locus_tag="PKO06_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV72657.1"
                     /translation="MKKSTVAILRTSPETVVRDYHDLLNLAGYQQVIDKSVDTALKIN
                     ISWHFFFPAASTTPWQLDGVIRALKADGYAPERMHGCHNRTVVIDAHLGERENKQLDV
                     IQTHGLRNVHLYEGEDWIDVRDAVGDLTKDFLCLNEVYPQGFSIPRRFIGENIIHLPT
                     VKTHVFTTTTGA"
BASE COUNT          272 a          410 c          369 g          235 t
ORIGIN      
        1 ctcgcgctga acggcatgaa aacgctgccg ggctatcgcg atgccattcg attagtcgcc
       61 aagaccgtcg agatgaaaac ctggcccgaa gctggcttgt actatccgca actcctgatc
      121 ttcccataca ccgcggcccg tgcctggcgc gatggcggag cgcgcgaacc cgaaatgcag
      181 gtggccatgg gaaaactgct gctcgacctg ctcgatctgc gcgaccgcta tgcccgcgaa
      241 aaaccccggc atcgcggagc gttccctggt ggcgaagacc gcagcgatca tctctcgacc
      301 gccctcggcc tgatcacgct gatcaacatc ggccaggaaa ccgcggagcg gatgggagtc
      361 tctgagcgct atacccaggc tctcgatgag gcagtgctct atctgatcaa gattggcgat
      421 gaagccaagc cgcataacca gaccaccgaa gcccgattgg gtcgccagac ccgaccgcgg
      481 cgctgggaca gcggcctgtt cttcgccgcc tcgttctggg atctcggcca ctggcgctca
      541 cagccgttca ccgtggccat ggttctcgaa gctctgacca agtatgcgct gctgcacgag
      601 cgttccggaa agcccttcgg cgcggcacgc ctgctcatac agcctgatcc cgcccgctcc
      661 gggcggctgg tgctggctcc gagtctctag tacaccaggt atcacggcga ccctgcaaca
      721 agggtacgag ttgccaatga cgattcagcg gaactgaaaa gagaaacacg atgaagaagt
      781 ctacggttgc gattctgcgc acgtcacccg agacggtcgt tcgcgattat catgatctgt
      841 tgaatctggc cggctatcag caggtcatcg acaagtcggt cgataccgcg ctcaagatca
      901 acatctcgtg gcacttcttc tttccggcgg cttcgacgac cccctggcag ctcgacggcg
      961 tgattcgcgc cctgaaagca gacggctacg cacccgaacg aatgcatgga tgccacaatc
     1021 gcaccgtggt catcgacgcg catctgggtg agcgagaaaa caagcagctc gacgtgattc
     1081 aaacccacgg gctgcgcaac gtgcatctgt acgaaggcga agactggatc gacgttcgcg
     1141 atgccgtcgg cgacctgacg aaggactttc tctgcctcaa cgaggtgtat ccgcagggct
     1201 tctcgattcc gcgacggttc atcggcgaga acatcattca cctgccgacc gtgaagacgc
     1261 acgtcttcac gacgacaacc ggggcg
//
LOCUS       DAORHD010001539         1285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150632_length_1285_cov_2.787805, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001539 DAORHD010000000
VERSION     DAORHD010001539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150632_length_1285_cov_2.787805"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            887..>1285
                     /locus_tag="PKO06_23315"
     CDS             887..>1285
                     /locus_tag="PKO06_23315"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV72658.1"
                     /translation="MKQPVHPSHEALPLSSVSGPDSYGRVQAIIRSLSGDRIRLIEAL
                     FQIQEIFGYIPDPVVGMLAQGVQLPRAEILSIATAIPGLFLKPRGRYVIRVCTGISCA
                     ICGNAGFLDVLRRQLKVKDHEVTKDGKFSIC"
BASE COUNT          300 a          318 c          330 g          337 t
ORIGIN      
        1 cgttgacctt gatggattca acggccatca ggggggcttc gaacgcgaag gtggcaatga
       61 ggacaaggca cagaaccttg ctaatcagtc ggatagtatt ttttgacata ggacttactc
      121 ctaactcctc ttgcgaattt tcccgatcca gcaaacgatt gatgccgttt gtaccctcct
      181 ttccttaccc ttaaacgttc acccgatttt cggacctacc cgtagagtgt acccaacact
      241 ctgtcaatca attatactga tcattcccgt ttacgcaacg gctcaaagaa tctttttgtg
      301 tgatcgccga aaatgcgctt gcagagcggt tgtgcaagtt gagattcagc aatggcgcgt
      361 gtggattata tgccgatctg tgagttgaca gtacgttacc atggcagaga cgagcatacg
      421 cgcagggctg cgcttctcta ccagagacga agaggtatca atgccgcctg cgattcgttg
      481 atggaggcct gatttcaagc gcgagtgtcc tttcgcccgg cgctctgtat ggttcgcact
      541 gggcaccgaa aaaatgggtg ggtcgcccgt atcaagcctc gtgaacgagt gctgtttggt
      601 tttcaaactg ggtctcaccc gggcctcaca gtcatggaat gtgcggtcgt ctcactcagt
      661 acagcataga tactcgtcta tattgccagt ggagcgtctc tgttctaaag aacctgcgct
      721 tcaaatagaa atcaagaact gcagaacttg tcgcaggttt cagaaatcca ccccggagga
      781 tgcaactcct tttcagatga tgtgaaataa atctcatatt tgcgaacgct cttcttgacg
      841 aacaaaccaa gatagtgtaa actttcacaa gtggaaagag aagccgatga aacaaccagt
      901 tcatcccagt catgaggcct tgccattgtc ttctgtaagt gggcccgaca gttacggcag
      961 ggtgcaggcg atcattcggt ctctgtctgg tgaccgtatc agattgatcg aggccctttt
     1021 ccagattcag gagatttttg gctatattcc tgacccggtt gtgggaatgc tcgctcaagg
     1081 cgttcagttg cctcgggcgg agatactctc gattgcgacc gcgatcccgg ggttgtttct
     1141 caagcctcgc gggcggtatg tgattcgcgt ctgtaccggc atcagctgcg ctatctgcgg
     1201 caatgccggc tttctcgatg ttctgcgccg gcagctgaaa gtgaaagacc atgaagtcac
     1261 caaagatgga aagttcagta tttgt
//
LOCUS       DAORHD010001540         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150825_length_1284_cov_3.172498, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001540 DAORHD010000000
VERSION     DAORHD010001540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150825_length_1284_cov_3.172498"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PKO06_23320"
     CDS             <1..402
                     /locus_tag="PKO06_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNV72659.1"
                     /translation="ADDNRMRKAFGQVRTYVAVDGSMADHPRTALYGALYSAVIANKA
                     DRRPTQSVAVAGKACESGDMIIYQAQVCDVEPRDILAVFCTGAYHYSMSSNYNRLTRP
                     AVVGIHEGKPRLLVKRETVNDLLRNDLNPYL"
     gene            483..>1284
                     /locus_tag="PKO06_23325"
     CDS             483..>1284
                     /locus_tag="PKO06_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72660.1"
                     /translation="MNARTPFSRVLMLAAGLLSICCGIAVAATIPHEEAQVQIWVPDG
                     WVQKSEGADLTAQTPKGDISIVYSVIEAENLKTALDQAEADIRKEVGEFEISEPKEKT
                     HNGMQMLDFDGTAMEGRMDLSISLFKTPSGKILAIYAFAAAGSVEKHQDDLIKMISEV
                     KPLDTDSARVSAPGRVPALEPVTPNDEPEPVGDGPAQSVESGGQMAPWAKGDSFYMNA
                     DNVNLRAEPNTNSATIMVIKAEQHGGGEVLGTGPKEVIGNHGANWWIKV"
BASE COUNT          303 a          353 c          387 g          241 t
ORIGIN      
        1 gccgacgaca accgcatgcg gaaggccttt ggccaggtgc gcacctatgt cgcggttgac
       61 ggttcgatgg ccgatcatcc gcgtacggcg ctctacggcg cgttgtacag cgcggtcatt
      121 gccaacaaag ccgatcggcg accgacgcaa tcggtggccg tggcggggaa agcctgtgag
      181 agcggcgaca tgatcatcta tcaggcgcag gtctgcgacg tcgaacctcg agacattctg
      241 gccgtgttct gcaccggggc ctaccactac tcgatgagct cgaactacaa tcggttgacg
      301 agaccggccg ttgtcggcat tcacgagggc aaaccgcgcc tgctggtcaa acgcgaaacc
      361 gtcaatgatc tgctgcgcaa tgatctgaat ccgtatctct gacacatgtg cgctttctgc
      421 ctatcatttt gaacctcaac atgtcatact ctgtaaatga catcaattca gggggttatc
      481 tcatgaacgc gcgaacgcca ttttcccggg ttctgatgct tgcggcaggg cttctctcga
      541 tctgttgcgg catagctgtg gccgcgacga ttccgcacga agaggcgcag gttcagatct
      601 gggttcccga cggctgggtg cagaagtctg aaggcgcgga tctgactgcg caaacgccca
      661 aaggtgatat cagcatcgtc tacagcgtga tcgaggcaga gaacctgaag accgcgctgg
      721 atcaagcgga agccgacatt cgcaaggaag tgggcgagtt cgaaatcagt gaaccgaaag
      781 agaaaacgca caacggcatg cagatgctcg acttcgacgg cacggccatg gaaggccgga
      841 tggatctttc catcagtctg ttcaagacgc catcgggcaa gattctggcg atctacgcct
      901 tcgcggcggc cgggtcagtc gaaaaacatc aggacgatct gatcaagatg atcagtgagg
      961 tgaagcctct tgataccgat tcggcccgtg tatcagcgcc cggtcgggtc ccggctctgg
     1021 aacccgtcac cccgaatgac gaacctgaac ccgtaggtga cggaccggcg caatccgtcg
     1081 aaagtggcgg acaaatggct ccgtgggcga aaggtgacag tttctacatg aacgcagaca
     1141 acgtgaacct tcgcgcagaa cccaacacga acagtgccac gatcatggtg attaaagcag
     1201 aacagcatgg gggtggagaa gtgctcggca ccggcccgaa ggaagtgatc gggaaccacg
     1261 gcgcgaactg gtggatcaag gtca
//
LOCUS       DAORHD010001541         1284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_150892_length_1284_cov_2.421481, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001541 DAORHD010000000
VERSION     DAORHD010001541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150892_length_1284_cov_2.421481"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..829)
                     /locus_tag="PKO06_23330"
     CDS             complement(32..829)
                     /locus_tag="PKO06_23330"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV72661.1"
                     /translation="MKASLRRLLRLLRKELAQTLRDPKMLGIIFFMPVLQLFIFGYAV
                     TTDINNIQTAVLDEDHSSASRDFLERLRGSGYFTIKRLLSRPSECDEVLDRAEVQFVL
                     RLPQGFARDLEAGTTTVVQTLFDGTDSMSAGIINGYVNQIIQEHAQQYLQRRLMRLKA
                     SLPSLPAVRAQIRVWFNPELKSVMFNVPGVLCLILFLVTMIMTALSIVKEKEIGTLEQ
                     IIVTPIRPWELILGKTIPYFLIGLIVTCLVISYAALARAARPIALPK"
     gene            complement(831..>1284)
                     /locus_tag="PKO06_23335"
     CDS             complement(831..>1284)
                     /locus_tag="PKO06_23335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV72662.1"
                     /translation="MVGKLIPYVLIAYGDVALVVLTSVFLFSVPLKGSLLLLFALSGT
                     FLTAALGVGLFISARAPNQQIANMAAMVGTQIPAVLLSGFMFPITSMPPSIQWIPNLI
                     PATHFIRILRGLFLKGNGLDVLKTHALILVVFATALVTLSALSFRKKL"
BASE COUNT          287 a          337 c          430 g          230 t
ORIGIN      
        1 aggcccgggc ggcgcggccc ggggataacg gttactttgg cagggcgatc ggccgcgcgg
       61 ctcgggcgag ggcggcgtac gagatcacca ggcaggtgac gatcaacccg atgagaaagt
      121 acggaatggt tttgccgaga atcagctccc atgggcgaat cggggtgacg atgatttgct
      181 cgagcgtgcc gatctccttt tccttgacga tggaaagggc cgtcatgatc atggtgacga
      241 ggaagaggat cagacagagg acacctggaa cattgaacat gacgctcttg agctcggggt
      301 tgaaccagac ccgaatctgc gcgcgaacgg cgggcagcga gggaagcgat gctttcagcc
      361 gcatcaggcg gcgctgcaga tactgctggg catgttcctg aatgatctga ttgacatagc
      421 cgttgatgat gcctgcggac atggagtcgg tcccgtcgaa cagggtttgc acgacggtgg
      481 tggttccggc ttcgaggtcg cgagcgaacc cctggggcag ccgcagaacg aactggacct
      541 cggcgcggtc gagcacctcg tcgcactcgg acggccgtga cagcaggcgt ttgatcgtga
      601 aatagcccga cccgcgcagg cgttcgagaa aatcgcgact ggccgacgag tgatcttcgt
      661 cgagcacggc ggtttggatg ttgttgatgt cggtcgtgac ggcatatccg aagatgaaca
      721 gctgcaacac cggcatgaag aagatgatgc caagcatttt cgggtcgcgc agcgtttgcg
      781 cgagctcttt gcgtaacagg cgcagcaggc ggcgaaggct ggctttcatg ttacagcttc
      841 ttgcgaaagc tcagcgcgct gagcgtgacc agagccgtgg cgaacaccac gagaatcagc
      901 gcgtgagtct tcaggacgtc gagcccgttg cctttcagaa acaggccgcg aagaatacga
      961 atgaagtgcg tcgccggaat cagattcggg atccattgaa tcgacggtgg catgctggtg
     1021 atcggaaaca tgaacccgga caacagcacg gctggtatct gtgtgccgac catggccgcc
     1081 atattggcga tctgttgatt gggggcgcgg gccgagatga acagtccgac accgagcgcg
     1141 gcggtgagaa acgtgccgct cagcgcgaac agcagcagca ggctgccctt gagtggcacc
     1201 gaaaacagaa agacgctggt aagcaccacg agcgcgacat caccgtaggc gatcagtaca
     1261 tacggaatca gtttgccgac catc
//
LOCUS       DAORHD010001542         1282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_151217_length_1282_cov_2.994295, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001542 DAORHD010000000
VERSION     DAORHD010001542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151217_length_1282_cov_2.994295"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKO06_23340"
     CDS             complement(<1..769)
                     /locus_tag="PKO06_23340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72663.1"
                     /translation="MSIGPRRLSMCRHGSVLVLGLGVLICLILFSVNFLQYSLRQKRL
                     GHRLGEHRLMTAPARALALLALHKLQFSPLLNSNSEAGGGPLPAPTAPLGRLFEMLSR
                     PLEQLEDLAESAETEIDLHDPATVNLDYVLQPLLQPLQQQGAVTGHVFASLRRDDFAE
                     CGLNRRGFSREKRGMIRLRVAMRFEKSGMTPIDEEFRFAVRVKITSALTAGLSKFTLY
                     LEKAAPTASANPFYGFNVVQTDEVGDLSRQVGSGIGHD"
     gene            complement(1065..>1282)
                     /locus_tag="PKO06_23345"
     CDS             complement(1065..>1282)
                     /locus_tag="PKO06_23345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72664.1"
                     /translation="GYCWASYLKVTETQPLAPNQPQSLPTLLEISDPATRLDHLEKGK
                     KSLPTANFSAIATLTTPPADPAAVPGY"
BASE COUNT          236 a          343 c          461 g          242 t
ORIGIN      
        1 catcatggcc gatgccggag cctacctggc ggctgaggtc gccgacctcg tcggtctgaa
       61 cgacattgaa cccgtagaat ggatttgcgg aagcggtggg agcggccttc tcgagataca
      121 gggtgaactt cgataacccc gcggtgagag ccgaggtaat ctttactcga acggcaaagc
      181 ggaactcttc gtcgatcggc gtcatgcctg acttctcgaa acgcatggct acgcgcagcc
      241 tgatcatgcc acgtttctcg cgggaaaacc cgcgtcgatt caggccgcac tcggcgaaat
      301 cgtcgcgccg cagcgaggcg aatacgtgac cagtcaccgc accctgttgc tgcagaggtt
      361 ggagcagggg ctgcaagaca tagtcgaggt tgacggtggc cggatcgtgc agatcgatct
      421 cggtctcggc cgattcagcc aggtcttcga gttgctcgag aggtcgtgag agcatctcga
      481 agaggcgccc gagcggagcg gtgggtgccg gcagcggtcc gccgcctgcc tcggagttcg
      541 agttcaggag cgggctgaac tggagtttat gcagagccag cagggccaga gcccgagcgg
      601 gggccgtcat cagccgatgc tcgccgagcc ggtgtccgag tcgcttctga cgcagactgt
      661 attgcagaaa gttgacggaa aagagaatca gacagatgag cacaccaagg cccaggacaa
      721 gaacgctgcc gtgacggcac atgctgagtc ggcgtggacc gatgctcacg cgcgcgagac
      781 ccgacgtcgg tgttggcctg tgtttgcaac cgtacccgtg gagtgagccg cgggtgtgga
      841 gcggtcgaga gcagggcctc cgaacacagt attcgtgttg tcagcggctc tcgctcgtgg
      901 ggaagtctgg gccatggcgc tggtctgccg tagcctgcgc cccgaagccc ttttgtgcaa
      961 gcgaaggttc gcctgtgtcc ggaggctgtg agacggcgga gggccgggcg gagttggagg
     1021 ttgagagtcg gtgaatgaga gagccgtcat ggccgtcggt gtggttagta tccgggaacg
     1081 gcggccgggt cggcgggcgg tgtggtcagc gtggcgatcg cggagaagtt cgcggtgggc
     1141 agcgacttct ttcccttctc cagatggtcg aggcgcgtgg ccggatcgct gatctcgaga
     1201 agagttggga gcgattgcgg ctgatttggt gccagaggct gggtttcagt gactttcagg
     1261 taggacgccc agcagtaccc ct
//
LOCUS       DAORHD010001543         1281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_151415_length_1281_cov_2.890701, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001543 DAORHD010000000
VERSION     DAORHD010001543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151415_length_1281_cov_2.890701"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PKO06_23350"
     CDS             <1..757
                     /locus_tag="PKO06_23350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV72665.1"
                     /translation="ERVLATRTDRLITLSETLKIDLLKRLRSTKPNHIQVVPLGLPLE
                     KLAASPRHTGRFRQSLGVDAKTCLWGIVARLVPVKNHALLLDTFARLARQHDTLHLAI
                     VGGGELADSLRNRVRKLGLSHRVHFTGIFHPIEDVYADLDLLLLTSLNEGTPVVLMES
                     LAAGCPAAATAVGGVADVFRDCGSGTLLPCDPEGLRRRLSVLVQSHSPAQSLSPEIRA
                     RVVERYSCTSLARRTAAVYAPLLAHRVPEYHQL"
     gene            844..>1281
                     /locus_tag="PKO06_23355"
     CDS             844..>1281
                     /locus_tag="PKO06_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV72666.1"
                     /translation="MADLFDIAGPPGAVQYKPLAERARPEALDGLMGQDHILGSGKPL
                     RQLLESGRCPSLLFWGPPGCGKTTLARIISRLVEAEFVQLSAVTSGVRELKECFEQAR
                     IRLNHHGRKTILFIDEIHRFNKAQQDALLPFVESGTVTFIGATT"
BASE COUNT          239 a          415 c          349 g          278 t
ORIGIN      
        1 cgaacgtgtt ctcgcaactc gcacggaccg tctgatcacg ctttcagaga ctctcaaaat
       61 cgatctgctc aaacggctgc gatcgacgaa accaaaccac attcaggttg tgccgctggg
      121 attacctctc gaaaagctgg ccgcttcgcc gcgccacacc ggcagatttc gccagagcct
      181 gggcgtggat gccaagacct gcctctgggg catcgtcgcg cgactggttc cggtcaagaa
      241 tcatgccctg ttgctcgaca ctttcgcccg tctcgcccgc cagcacgata cacttcatct
      301 cgccatagtt ggtggcggag aacttgctga tagtctgcgc aaccgtgttc ggaaactcgg
      361 actttcacat cgcgttcact tcaccggcat ctttcacccg atcgaagatg tgtatgccga
      421 tctcgatctg ctgcttctga cctccctcaa cgagggaacc ccggtcgttc tgatggaatc
      481 gctcgcagcc ggctgcccgg cggcggccac cgccgtcgga ggagttgcgg atgttttccg
      541 cgactgcggc agcggcactc ttctgccgtg cgaccccgag gggctccgac gtcgtttgtc
      601 cgtgttggtc caatcgcatt ctcccgcgca atcgctttcg ccagagatca gagccagagt
      661 cgttgaacga tattcctgta cgtcactcgc ccgacgaacc gcggcagtct atgcccccct
      721 gctggctcat cgcgttcccg aataccatca gctctgatcg ccctttcgac gaacgcggac
      781 tccgacgcct gatgagttgc attgccggtt gcctgctgtg tgcgagtgca gtagcataca
      841 gtcatggctg atttgttcga catcgccggt ccgcccgggg ccgtgcagta caaacctctg
      901 gctgaacgag cccgccctga agctctcgac ggcttgatgg gtcaggatca cattctcggt
      961 tctggcaagc cgcttcgcca gctgctcgaa agcggaagat gcccttcatt gctgttctgg
     1021 gggccacccg ggtgcggcaa gaccacgctc gcccgcatca tctcacggct cgtcgaggcg
     1081 gagttcgtgc aactgtccgc cgtgacttcc ggggtacgcg aactgaagga atgctttgaa
     1141 caggcccgca ttcgattgaa ccatcatggg cgaaaaacca tcctgttcat cgatgaaatt
     1201 caccgtttca acaaagccca gcaagacgcc ctgctgccgt tcgtcgagtc agggacggtc
     1261 acgttcatcg gagccacgac c
//
LOCUS       DAORHD010001544         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_151568_length_1280_cov_3.990204, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001544 DAORHD010000000
VERSION     DAORHD010001544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151568_length_1280_cov_3.990204"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKO06_23360"
     CDS             complement(<1..470)
                     /locus_tag="PKO06_23360"
                     /inference="COORDINATES: protein motif:HMM:NF024674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HNV72667.1"
                     /translation="MKILFIGDIYGRAGRTLVSRELPALRQKLACDWVVANAENVSGG
                     VGLSPGHFKELCRAGVDVCTMGNHLFARRDWPDLVKDERVLRPHNLGGDALPGKGWGW
                     YQGENGKALGVINVAGRVFMEPAECPFRWVEALLERLPHKMPVICDFHAEATSE"
     gene            complement(581..>1280)
                     /locus_tag="PKO06_23365"
     CDS             complement(581..>1280)
                     /locus_tag="PKO06_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007934275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNV72668.1"
                     /translation="YEKAKAEVELRIKEAGEQALLQLGIQSMHSELVDIIGRLNYRTS
                     YGQNVLQHSIEVATLAASLAAEIGADVQLAKRSGLLHDIGKVIESDEGNHASLGADFA
                     RKYQESPRVVNCIGAHHEDLPFESAEAVLIAAADAISASRRGARHESLDAYVKRLSDL
                     ENVARSFPGVASVYAIQAGREVRVIVNPEQIDDNVAPKLCRDIVKKIEGEMEYPGQVK
                     VVLIREVRNVEVAH"
BASE COUNT          284 a          419 c          326 g          251 t
ORIGIN      
        1 ttttcagatg tcgcctcggc atgaaagtcg cagatcaccg gcatcttgtg aggcaaccgc
       61 tccagcaggg cctcgaccca gcgaaacgga cactcggccg gttccatgaa aacgcgaccg
      121 gcaacattga tcacaccaag ggcttttccg ttctcgccct ggtaccaccc ccatcctttc
      181 cctggcaatg cgtcaccgcc cagattgtgg gggcgcaaaa cacgttcgtc tttcaccaga
      241 tccggccagt cacggcgggc aaagagatga tttcccatgg tgcagacatc gactccggct
      301 cgacacagtt ccttgaaatg accgggagac aacccgacac caccgctgac gttttccgcg
      361 ttcgcgacca cccagtcaca agccagtttc tgccgcaatg ccggaagctc ccgactgacc
      421 agggttcgac cggcccgacc atagatgtcg ccgataaaca gaatcttcac gtccatctcc
      481 gtactcgttg atcaattgcc gaaacagctt ctatcaacgg gaagcgagtg aaagaaaaac
      541 aggcggaacc gggatacccc ggcccgcctg cagaccatca tcaatgagca acttcgacgt
      601 tgcgcacctc gcggatcagg accaccttga cctgacccgg atactccatc tcgccttcga
      661 tctttttcac gatatcgcga cacagtttcg gagccacgtt atcgtcgatc tgttccggat
      721 tcacgataac ccgcacctcg cgacccgctt gaatggcata gaccgaagcc acgcccggga
      781 agctgcgtgc aacattttcc agatcggaca aacgcttcac ataggcatcg agagactcgt
      841 gacgcgcgcc acggcgtgac gccgaaatgg catcggctgc cgcgatcaag acggcctcgg
      901 cgctctcaaa cggcagatct tcatgatgag cgccgataca gttgactacc cgcggtgatt
      961 cctgatactt gcgggcaaag tcggcaccca acgaggcatg gttgccctcg tcgctctcga
     1021 tcaccttgcc aatgtcatgc aacaaaccgg agcgtttggc cagctgcaca tccgcgccga
     1081 tttccgccgc gagacttgcc gccagggtcg cgacctcgat ggagtgctgc agaacgttct
     1141 gaccgtagga cgtgcggtag ttaagccgac caatgatgtc gaccagttcc gagtgcatcg
     1201 actggatgcc cagctgcagc agagcctgtt caccggcctc cttgatacgc agttcgacct
     1261 cggctttcgc cttctcgtac
//
LOCUS       DAORHD010001545         1280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_151570_length_1280_cov_3.948571, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001545 DAORHD010000000
VERSION     DAORHD010001545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151570_length_1280_cov_3.948571"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1280
                     /gene="cas10"
                     /locus_tag="PKO06_23370"
     CDS             <1..>1280
                     /gene="cas10"
                     /locus_tag="PKO06_23370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HNV72669.1"
                     /translation="AEFNAAEWGLSDPFINLAAGHHKPETPMQWIITEADRLASGIDR
                     EKYEERNHMADVAKFRVTRMLCPFEEIELPETEDKRKKFKSADDFKWRLPLREVSACA
                     LFPVLRAEAENETSATKEYGDLFFKFVAALEKLEHQDSLALWYEHFDSLYGIYAGLIP
                     AATVDVLADVSLYDHSRAVAAFSAASYQYHQAKGTMTIDAIRDKEEKKYQLILGDFFG
                     IQNFIFSEGGQTRRNAAKLLRGRSFYVSLLTELAADFLLRELGLPVTSLLINAAGKFA
                     ILAPNLEQVKTAIARVRKQVNDWVVKHFMGQASLGIVCIEAAPCDFENGRWSDLWNQA
                     SRALSAVKSRRIDLEAYGGVQHGFIEQFGEFGLCPFCGQRPGDSWVRVGVDNDKVACC
                     GICADQMEMGRSLVRKETRYLAITEIDADLRKEG"
BASE COUNT          294 a          325 c          385 g          276 t
ORIGIN      
        1 tgcagagttc aacgcggcgg aatgggggtt gagcgacccg ttcatcaacc tcgccgccgg
       61 tcaccacaag cccgagactc ccatgcagtg gatcatcacg gaagcggatc gtctggcaag
      121 cggcatcgat cgagagaaat acgaagagcg aaatcacatg gccgatgtgg cgaaattcag
      181 ggtcacccgc atgctctgcc cgttcgaaga gatcgaacta cccgaaactg aggacaagcg
      241 caaaaagttc aagtcagccg acgattttaa gtggcgctta ccattgcgtg aggtatcggc
      301 atgcgccctt tttccggtct tgcgtgcaga ggcggagaac gagacgagtg caacgaagga
      361 atacggagac ctgttcttca aatttgtcgc cgcgcttgag aagctcgaac atcaagacag
      421 cctggctttg tggtacgagc atttcgacag cttgtacgga atctatgccg gcctgattcc
      481 ggccgcgaca gtcgatgtcc tcgccgatgt ctctctgtac gatcactcgc gtgcggtcgc
      541 ggcgttctcg gcggcctcgt atcagtatca tcaggcgaag ggcacgatga cgatcgacgc
      601 gattcgcgac aaggaagaga agaaatacca gctgattctt ggcgacttct tcggaattca
      661 gaatttcatc ttctccgaag gtggccagac gcgtcgcaat gccgcgaaac tgttgcgtgg
      721 tcgatcgttc tatgtatccc ttctcaccga gctggccgcg gattttctgt tgcgcgaact
      781 cggacttcca gttacttcgc tcttgatcaa cgcggccgga aagttcgcca ttctggcacc
      841 gaatctcgaa caagtgaaga cggcgattgc gcgggtgaga aaacaggtga acgattgggt
      901 ggtcaaacat ttcatggggc aggccagcct tggcattgtc tgtattgaag ccgctccgtg
      961 cgatttcgaa aacgggcgat ggagcgattt gtggaatcag gcatctcgcg ccttgtcggc
     1021 ggtcaaaagc cggagaattg atctcgaagc atacgggggg gttcagcatg ggttcatcga
     1081 acagttcggc gagttcggcc tgtgcccgtt ctgtggccag cggcccggag acagctgggt
     1141 gcgagtcggt gtcgataacg ataaagttgc ctgttgcgga atctgtgctg atcaaatgga
     1201 aatgggcaga tcgctggtac gaaaggaaac gcgatatctg gccatcacgg aaatcgatgc
     1261 cgatcttcgc aaggaagggc
//
LOCUS       DAORHD010001546         1278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_151999_length_1278_cov_3.122649, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001546 DAORHD010000000
VERSION     DAORHD010001546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151999_length_1278_cov_3.122649"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKO06_23375"
     CDS             complement(<1..441)
                     /locus_tag="PKO06_23375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72670.1"
                     /translation="MKWSATGYVERLSIRLTRWLIVLVAFGLMPLGIINYGVRQLTED
                     RWLRYVDSQAARLGTIMENAQKTHTPENFYYTVLAQLNDKLDAMDQNLEARRELAQRF
                     TTRWRGVFDVFALDASGSLAFPPDLPGGISLFSLKKLAEALRELP"
     gene            750..>1278
                     /locus_tag="PKO06_23380"
     CDS             750..>1278
                     /locus_tag="PKO06_23380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNV72671.1"
                     /translation="MNTQNQHLHPAIADLRKGRAGRCKIVSAAEAVSIIRDGDTIATG
                     GFVGTGFAEDLAVALEKRFLESGGPRHLTLVYAAGQGDGKERGLNHFGHEGLIKRVIG
                     GHWGLVPKLQKLAFADKIEAYNLPQGVISHLYRDIAAHKPRTITSVGLGTFVDPRLTG
                     GRINPCTKEDLVEVVQ"
BASE COUNT          291 a          370 c          361 g          256 t
ORIGIN      
        1 cggcagttca cgcagagctt cggccagctt tttcaacgaa aagagtgata ttcctcccgg
       61 aagatcgggc ggaaacgcga gcgagcccga ggcatcgagt gcgaagacat cgaatactcc
      121 acgccagcgt gtcgtaaaac gttgtgcgag ttcgcgacgg gcttcgagat tctgatccat
      181 cgcgtccagt ttgtcgttca gctgtgccag caccgtgtaa tagaaattct ccggagtgtg
      241 cgtcttctgg gcattctcca tgatcgtccc gaggcgcgcc gcctgtgaat cgacatagcg
      301 aagccaacga tcctcggtca gttggcgaac accgtaattg atgattccaa gcggcatcag
      361 gccaaaagcc accaggacaa tcaaccaacg ggtaagacga atgctcaggc gctccacgta
      421 gccggttgca ctccatttca cagtagtcag gcatctctca accctgcggt tccgagaaac
      481 agcgctgcga caacgcccat gatatcacgg atggagcgtc gtcacgactg tcactcggga
      541 gcacccaggt catgtgattc tgtccagttt tcctcttggc gtgagcgaat ctttgagcga
      601 tgccaagggg aaaccccaaa ccggagaatc aattgacccc gggctcgggc tggtaacact
      661 tgtcaaaccg gcacaaaatg cctacactac ctggcagttc aagcgcagac gactgtcatg
      721 cgccggtgag atctctcgcg gaggaagcca tgaataccca gaaccaacac ctgcacccgg
      781 caatcgccga tttacgcaaa gggcgcgccg gtcgctgcaa aattgtctcg gctgctgaag
      841 ccgtttcgat cattcgcgac ggagacacga tcgccacggg cggttttgtc ggcacgggtt
      901 tcgcggaaga tctggcggta gcgctcgaaa agcgctttct cgagtcgggc gggccacgac
      961 atctgacgct ggtttacgcc gccggtcagg gcgacggcaa ggagcgtggc ctgaatcatt
     1021 tcggtcacga gggtctgatc aagcgcgtga tcggcggtca ctggggtctg gttccgaaac
     1081 tgcagaaact ggcttttgcc gacaagatcg aggcctacaa cctgccacag ggagtcattt
     1141 cccatctgta tcgcgacatt gccgctcata aaccccgaac catcacgtcg gtcggtctgg
     1201 gcacttttgt tgacccgcgc ctgaccggag gcagaatcaa cccttgcacc aaagaagacc
     1261 tggtggaagt cgttcagt
//
LOCUS       DAORHD010001547         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_152403_length_1276_cov_2.892711, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001547 DAORHD010000000
VERSION     DAORHD010001547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152403_length_1276_cov_2.892711"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PKO06_23385"
     CDS             complement(<1..665)
                     /locus_tag="PKO06_23385"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNV72672.1"
                     /translation="MIQTGLAIASGRDTADVARLVVDRALSQLSTPTFAVLFSSLSYD
                     QKILFDTIRARLGPIPIFGTTSSILISSRGSEPDSVLLLLLQAPDLEFSILSGKCGRN
                     PAEIGSYLASRYLFDAKPTAGDLVSCILTGTEKHHSSFSYLHGLYSVFPWRLPVTGGT
                     TVGRFPSEVYDDIFIGNQYCGNLLTQDHLALLFLRARMRRKRLMRSKPGSSRATWASR
                     NTRS"
     gene            829..>1276
                     /locus_tag="PKO06_23390"
     CDS             829..>1276
                     /locus_tag="PKO06_23390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV72673.1"
                     /translation="MSLEQKLGDFEILKDFTTKELKLMATFMETRDYADGEEIIGKDN
                     ANHSLFFILSGKVRIYRNFSGGASFSTQIGTKEVFGEVAFADQQGRTALAEAVGAVEI
                     ALFDYDHFNIIKKQDPVFGMKLLLELLKLLATKFRVVNKKLDSIFSQ"
BASE COUNT          315 a          310 c          378 g          273 t
ORIGIN      
        1 gatctggtat tccgcgacgc ccatgtcgcg cgactcgatc caggcttcga gcgcatcaag
       61 cgttttctgc gcatgcgtgc gcgcaggaag agcagggcca ggtggtcctg ggtgagcagg
      121 ttgccacaat actgattgcc gatgaagata tcgtcgtaga cctctgaagg aaagcggccg
      181 acggtggttc ctccggttac aggaagacgc cacggaaaaa cagagtacaa gccatgtaaa
      241 taggaaaagc ttgagtgatg cttctcggtg ccggtaagaa tgcaggaaac aaggtcgccg
      301 gccgtcggtt tggcgtcgaa gagatagcga gaagccaggt aactgccgat ttctgccgga
      361 ttcctgccgc actttccgga gagaatcgaa aactcgagat ctggggcctg aaggagcagc
      421 agaagaacgg agtccggttc gcttccacgg cttgagatca agatgctcga agttgttccg
      481 aagatcggta tgggcccgag acgtgcgcga atcgtgtcaa acaggatttt ctggtcgtag
      541 gagagagacg agaacagcac cgcaaacgtt ggcgtggaaa gttgggacaa tgcccgatcg
      601 acaaccagcc gagcgacgtc ggctgtgtcg cgaccactgg caatcgctaa tccggtctga
      661 atcatacgac ctccgtgaag cttgaaccac tgtccgcatt gtagcagata gtgatcacac
      721 aattcctgtc ggttgtctca gaaaaaccag ggcgtacagg cttgtgtctc gaacctgact
      781 gagctacatt acgatacggc tgatttcatt tctgtgagga ggaaagcgat gtcactggaa
      841 cagaagctgg gagactttga gattctgaag gatttcacca cgaaagagct gaagctgatg
      901 gctactttca tggagactcg ggactatgcg gatggtgagg aaatcatcgg gaaggacaac
      961 gcgaaccatt cgctgttttt catcctttcc ggcaaggtgc gcatttaccg gaacttttcc
     1021 ggcggcgcca gtttctcgac tcagatcggc accaaagaag tattcggtga ggtagccttt
     1081 gctgaccagc aggggcgaac cgcgctcgcc gaggccgtcg gtgcggtcga gattgccttg
     1141 ttcgattatg atcacttcaa catcatcaag aaacaggacc cggtgttcgg catgaaactg
     1201 ttgctcgaac tgctcaagct gctggcgacc aagttccggg tggtaaacaa gaagctcgat
     1261 tcgatctttt cgcaga
//
LOCUS       DAORHD010001548         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_152445_length_1276_cov_2.506143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001548 DAORHD010000000
VERSION     DAORHD010001548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152445_length_1276_cov_2.506143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1276
                     /locus_tag="PKO06_23395"
     CDS             <1..>1276
                     /locus_tag="PKO06_23395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72674.1"
                     /translation="KAAAKPVAPSAKTKAELPAGESINAAPLPEASQTKPVIVGGKLV
                     SLPPRPTPAAVVVEEVGDDIDLMAAAAADPQKVSVSAAPAAPAAPEPSPAGAEDLTNR
                     WEYLKRKAAAQAIPPATTVGTARVTTPPPVQSPMVPPAPAPMAVSSRVAVSPEAVAYA
                     PATAAPVPNTPWNRASIGSPTGPIPPAAASAPVNLGPFPPVLEENGISLDFSPERRQD
                     DLISKLKMLAIEDGELRALLLQKNPSHLPALIEYVMLRPEPVRLAFVARELGHYHDPA
                     VTDVLAQLLYHEDVRVVLAAIQGIQLNADPSGILHVSPFISSPDGILSEAARNALMSY
                     GPAKVLQMYARELPRNADDRIREGGVFVLSRMKGGAVTNLLVQMLADRSVEVRKKVIM
                     AMTFQKDPQYLPVLREFMKAAPEEEKKLARKAV"
BASE COUNT          256 a          396 c          372 g          252 t
ORIGIN      
        1 cgaaagcggc ggcaaaaccg gttgctccgt ctgccaaaac gaaagccgag ctccctgccg
       61 gggagtcgat caatgcagca ccattgcctg aagcaagtca gacgaaaccg gtgattgtcg
      121 gcggaaaact ggtctctttg ccgccacgac cgacccctgc ggctgtggtc gttgaggaag
      181 tgggcgacga catcgatctc atggccgcgg ctgcggctga tccgcagaaa gtgtccgttt
      241 ccgcggctcc tgccgccccc gccgcccccg aaccatctcc tgcgggagcg gaggatctca
      301 ccaatcgctg ggagtacttg aaacggaaag ccgcggcaca ggcgattccg ccggcgacga
      361 ccgtcggaac cgcgcgcgtg acaactccac cgccggtcca gtctccgatg gtgcctcctg
      421 cacccgcacc aatggctgtc agttctcgag tggctgtttc ccctgaggcg gtcgcctatg
      481 ctccagcaac ggcagcgccc gtaccgaaca ccccctggaa tcgcgcctcg atcggttctc
      541 cgactggtcc gatcccgcct gccgctgcgt cggcacccgt gaatcttggt ccttttccgc
      601 ccgtactcga agagaacggc atctcgctcg acttttcccc ggaacgccga caagatgatc
      661 tgatcagcaa gctgaagatg ctggccatcg aggatggaga gctgcgtgcc ttgctgttgc
      721 agaaaaaccc gtcacatctt ccggcgttga ttgagtatgt catgcttcgc ccagagccgg
      781 taagactggc ttttgtggct cgtgaactcg gacactatca tgacccggcg gtcacggatg
      841 tgttggcgca actgctgtat catgaagatg ttcgcgtggt gctggcggcc attcaaggta
      901 ttcagctgaa tgccgatccc tcgggaatcc tgcatgtcag tcctttcatt tcctcaccgg
      961 acggcattct cagcgaggcg gcgcgcaatg ccctcatgtc ctacggaccg gcaaaggtgc
     1021 tgcagatgta tgcgcgtgag ctgcctcgca acgccgacga tcgtattcgt gaaggcggag
     1081 tattcgtcct ttcacgcatg aagggcggcg ccgtgaccaa tctgctggtg cagatgctgg
     1141 ccgaccgctc tgtcgaagtg cgaaaaaagg tgatcatggc gatgaccttc caaaaggacc
     1201 ctcagtatct gccagtcctg cgggagttca tgaaagccgc accggaagaa gaaaaaaagc
     1261 tggcacgcaa agccgt
//
LOCUS       DAORHD010001549         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_152471_length_1276_cov_2.171990, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001549 DAORHD010000000
VERSION     DAORHD010001549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152471_length_1276_cov_2.171990"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1122
                     /locus_tag="PKO06_23400"
     CDS             172..1122
                     /locus_tag="PKO06_23400"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV72675.1"
                     /translation="MTPHITISLTKTGWGLILGSLFLYSSARSNDNNLLYLIGAASFA
                     IVVSHFIAGWRNLSGLSVSWQPPDEGFVGSDLNPVLLIDEKAQRSHFQLSMGQDYLEN
                     LGPGQTFSFHGHFRLERRGCERFSQHCLWSLYPFGLARFTVNLPPVTVWAYPQPQECQ
                     IFGVQESMQSSRQPAVDSMGDYWMLREYREGEDARQINWHSSAKTDAEWILVLAQPRQ
                     EPFRIWIDFRGLQDEALETFFRRISGLILRLADQPIAMQLWVAKPPVPFLPGWFDLRE
                     GPNRRIAWRWLAGLNAQDAESSVPPNAHEGFSLSASHLSP"
BASE COUNT          240 a          388 c          344 g          304 t
ORIGIN      
        1 cgagttggtc gcgcggcaac aggcggtcgt cgacattgat ctggtcaagt atctggcccc
       61 acgcgtgttc ggccatcgac tcagtctgcg aactcccgtg aacgctctcg tagaagacac
      121 atcgcaggcg agctgggttg aacgcgagtt gctgatgcga gttcccgttg agtgacgcca
      181 cacatcacca tctccctgac gaaaaccgga tggggactga tcctcggcag tctctttctg
      241 tattcctccg cgcgcagcaa cgataacaat ctcctctatc ttattggcgc ggcctctttc
      301 gcaatcgtcg tcagccactt catcgccggc tggcgcaatc tttctggtct gtccgtgtcc
      361 tggcagcctc cggacgaagg ctttgtcgga agtgatctga acccggtgct cttgatcgat
      421 gagaaggcac agcgcagcca cttccagctc tccatgggcc aggattacct ggaaaatctg
      481 ggaccggggc aaaccttctc ctttcatggt catttcagat tggaacgccg gggatgcgaa
      541 agattttctc agcactgcct ctggtcactt tatcctttcg gcctggcccg tttcacggtc
      601 aacctgcctc ccgtaaccgt ctgggcttat cctcagccgc aggagtgcca gatcttcggg
      661 gttcaggaat ccatgcagag cagtcgccaa ccagccgtcg actcaatggg cgattactgg
      721 atgctgcgcg agtatcgcga aggcgaagat gcgcgccaga tcaactggca ctcgtcagcc
      781 aaaacagatg cggaatggat tctggtgctg gctcaacctc gacaggagcc gtttcgcatc
      841 tggatcgatt ttcgtgggtt gcaggatgaa gctctcgaga cgttcttccg cagaatctcc
      901 gggctgattc tgcgactggc tgaccagcca atcgcgatgc aattgtgggt ggcaaaaccg
      961 ccggtgccct ttcttcccgg atggttcgac cttcgcgagg gtccgaaccg ccgtatcgcc
     1021 tggcgatggt tagccggtct gaatgcacag gatgccgaat cctccgttcc accgaatgcg
     1081 cacgagggtt tttcgttgag cgcttcacac ctgtcaccat gaaacagacc ctgcgttcgc
     1141 tcacccgact tctgccttcg gttaccgagt ggcgatttca catcgcgctt tcgtgcctga
     1201 tcggctacct gacattcttg actacaggcg ccaaccgtct cttcgaagcc cacgcctttc
     1261 tcgttgcact cgtcgt
//
LOCUS       DAORHD010001550         1276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_152472_length_1276_cov_2.163800, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001550 DAORHD010000000
VERSION     DAORHD010001550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152472_length_1276_cov_2.163800"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1138)
                     /locus_tag="PKO06_23405"
     CDS             complement(110..1138)
                     /locus_tag="PKO06_23405"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV72676.1"
                     /translation="MKRLVRIVLTLFLIAGALCLFVAEVVLRQMVRQPHQDPVGRSLL
                     LAALKKAGTDTGAVELGPEWTALTSSDTRRLADPGLKAFSALNVRGQRIWGWYYPGQP
                     DAGAILLCHGHSVSHNELRYVFNFLRPTGYHLLMLDFRAHGASDGEYTSIGLQEWEDV
                     DAILKEAEKLGYLPPNRPLGAYGRSMGAATLVNGARHLPRIHAFLIEAMFPWLRRIGA
                     NDLKAYVGIPDNPFVDVAFWYASWRTGIDYLTNRPENEIGNIAPRPLLIIHDAKDVRL
                     HRSDFERLKTAASGAQTLVFDEAGHVGAHKSEPERFEREFLSFFAAAGIPRPAGTEIV
                     STSSLDLK"
BASE COUNT          291 a          416 c          320 g          249 t
ORIGIN      
        1 gtgacagacc agcacctcgc ccctgccgat cttctcatac ttccagagct tggctcgaca
       61 agcggcacac cgaatcaaga gcatgacatc tctcctgctc tttcagagct tatttgagat
      121 caagagagct cgtactgacg atctccgtgc ccgcagggcg aggaattccg gctgctgcaa
      181 agaacgacag aaactcacgt tcgaaacgct ctggctcgga cttatgcgcg ccgacatgac
      241 ctgcttcatc gaacaccagc gtttgcgcac ccgaagccgc cgttttcaag cgctcgaaat
      301 cggacctatg caatcgcaca tccttcgcat cgtgaatgat cagcagtggt cgcggcgcga
      361 tgttgccaat ctcgttttca gggcggttcg tcaggtagtc gatgcccgtg cgccacgagg
      421 cataccagaa cgccacatcc acaaacggat tgtccggaat gccgacatac gctttcaaat
      481 cattggcccc gatgcgccgt aaccagggaa acatggcctc gatcagaaac gcatgaatgc
      541 gcggcagatg acgtgcaccg ttcaccagcg tcgccgctcc catcgaacgc ccataggcgc
      601 ccagtgggcg attcggcggc agatatccga gtttctccgc ttccttcagg atcgcatcga
      661 catcctccca ctcctgcaga ccgatcgacg tgtattcccc gtcactagcg ccatgggccc
      721 tgaaatccag catcagcaga tggtaccccg tcggcctcag gaagttgaac acataccgca
      781 actcgttgtg actgacactg tgaccgtggc acagcagaat ggcccccgca tcgggctgtc
      841 ccggatagta ccacccccag attcgctgcc cccggacgtt caacgcggaa aaagccttga
      901 gcccaggatc agccaaccgt ctcgtatcac tggaagtcag agcggtccac tccggtccga
      961 gctcgaccgc gccggtatcg gttccggcct ttttcagcgc cgccaacaac agcgacctgc
     1021 cgacgggatc ctggtgcggc tgtcgcacca tctgtcgcaa cacgacctct gccacgaaaa
     1081 ggcagagggc tccagcaatc aggaacaacg tcaggacgat tcgtaccagt cgcttcatat
     1141 ggaattccct tctcgataag cctctcagcg cttgaggaat ttcagatgct gattgaacga
     1201 aaacagttca tcctgcacct tggcaaactc cggagcaaag tccatcttca gcgtgatgcc
     1261 ctccgcccga cagaga
//
LOCUS       DAORHD010001551         1272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_153218_length_1272_cov_2.958915, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001551 DAORHD010000000
VERSION     DAORHD010001551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153218_length_1272_cov_2.958915"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1272
                     /locus_tag="PKO06_23410"
     CDS             28..>1272
                     /locus_tag="PKO06_23410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72677.1"
                     /translation="MKARVTIVAVDTFRIAGESAPYGGQQYHGRLLNHLEIPGLLDCG
                     ESGNPVRSLSIDVRNIDNWIRPAVQNLWGAIVIVDVDGINETWVGKVTGYNQSRTGVL
                     SIDAGEDSLAIFKLPLPDETVHLINWPDAEGEAIDTAVNMPFGGTVAQPVRVKAILVD
                     RVRFIYLISVGTIRQVVRVLKNREELADGYSTTLGTEVQALYPGYAYVRFDADPRDSS
                     GNWPEVLVDVLGLVVGSVDVTECRNPARILKHLLITPRTGACGWGLGVPQSMINEAAF
                     DQAIADCDDAGLLLDGCFFAKREAAYWLNELWMVGRIKLTIEAGKWTPRVEKPGVSER
                     TFTGNSLQPVSWGKGASNERKNLVKLYYFWDISRDRLIRSVKRNDPTGHAGSQDRLGI
                     QETEVEAVLVQDATTAQKICDFL"
BASE COUNT          257 a          361 c          397 g          257 t
ORIGIN      
        1 gttgagctac gctttccggg agaaaccgtg aaggcccgcg tcacgatcgt cgccgtcgat
       61 acgtttcgca tcgccggtga atccgctccg tatggcggtc agcagtatca tggccggctg
      121 cttaatcatc tcgagattcc gggcctgctc gactgcggtg aaagcggaaa ccccgtgcgc
      181 agtctctcga tcgacgtgcg aaacattgac aactggatca gaccggcggt gcagaacctc
      241 tggggcgcga tcgtcatcgt cgatgtcgat ggcatcaacg aaacgtgggt cggcaaggtc
      301 acgggctaca accagagccg aaccggggtt ctctcgatcg acgcgggcga ggattccctg
      361 gcgatcttca aactgcctct gcccgatgag acggtgcatc tcatcaactg gcctgatgcc
      421 gaaggtgagg caatcgatac cgccgtcaac atgccgttcg gcggcacggt tgctcagccg
      481 gtgcgggtga aagcgattct cgtcgatcgc gtgcgcttca tctacctcat cagcgtcggc
      541 accattcggc aggtcgtccg agttctgaag aaccgtgaag agcttgctga tgggtattca
      601 accactctcg gcaccgaagt gcaggcgttg tatccgggtt atgcctatgt gcgcttcgat
      661 gccgacccgc gtgacagctc tggaaactgg cctgaagtgc tggtggacgt gctcggtctg
      721 gtcgtcggct cagtcgatgt gaccgaatgc cgaaaccccg ctcgaattct gaaacatctg
      781 ctgatcacgc cgagaaccgg tgcgtgcggc tgggggttgg gcgttccgca gagcatgatc
      841 aacgaggccg cgttcgacca ggcgatagcc gactgcgatg atgccgggct gctgctggac
      901 ggatgtttct ttgccaagcg cgaagccgcc tactggctga acgaactctg gatggtcggt
      961 cggatcaagc ttaccatcga ggccggaaag tggactcctc gggtcgaaaa gcctggtgtg
     1021 tcggagcgca ccttcaccgg aaactcgttg cagcccgtca gctggggaaa gggcgcgtca
     1081 aacgagcgca agaacctcgt caagctctat tacttctggg acatcagccg tgatcgtctc
     1141 atccgctctg tgaaacgcaa tgacccgaca gggcatgccg gaagccagga cagacttggt
     1201 attcaggaaa ccgaagtcga ggccgttctg gtgcaggatg cgacgacggc gcagaagatc
     1261 tgcgactttc tg
//
LOCUS       DAORHD010001552         1271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_153361_length_1271_cov_4.567434, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001552 DAORHD010000000
VERSION     DAORHD010001552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153361_length_1271_cov_4.567434"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..>1271
                     /locus_tag="PKO06_23415"
     CDS             281..>1271
                     /locus_tag="PKO06_23415"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNV72678.1"
                     /translation="MKKHLFVAVILLGIVSLLQPAAVSAQSPYNDDVVVTPNVAVSDT
                     TQPVAPLTVASDTSRTEEEVASLSRSLWKELRRLYKDSYIGRRGRRDPGKEVCTPTMH
                     HLPGYDNMNIDIDGDNYCGQFAMSTLLNGMGIQTDPQKVYKDTNPAGIFTAPPVIVEY
                     LRSKGVDARMKNRASVSDITKRIDQGKPVMVLVDSGDGTPHWICIYGYDTDASGKVTS
                     VRMRDSYWGTDGPHTMPIADFEKAWRKPFGKGILGDLMSYSNVLIDNNGTMNPSNPLF
                     PGTFGTATEDNIAGAINDVVTGWQNHNVAQVVGGVTKAIVGLPGAITGVASNFL"
BASE COUNT          274 a          367 c          352 g          278 t
ORIGIN      
        1 gccgtacagg cccaggaact cctgcgcaag catcaggaga agattgcccg actcgaagcg
       61 tatcaggttc ccattggcat tgcctgtctg atacttggct tcctgcatct ggtgctgccc
      121 ggctggtggt tgttctgatc tgatctcgaa acgtcacggg gcgactgctg tatcagtcgc
      181 cccgtttttt ttcgttgggt tctcccgtgc gagaaagtga aaactctcag ggcgcaggct
      241 ggtatgatag acaaagaaca ctctgggaaa ggggcgtacc atgaaaaagc atctcttcgt
      301 cgcggtcatt ctcctcggaa tcgtctcact gttgcagccc gccgctgtgt cggcgcaatc
      361 gccgtataac gacgatgtgg tcgtgacacc gaacgtggct gtctccgata cgactcagcc
      421 tgtcgctccg ctgactgtcg cctccgatac ttccagaacc gaagaagaag tcgcctcgct
      481 gagccgctct ctctggaagg aactgcgccg gctctataaa gactcataca tcggtcgtcg
      541 cggtcgacgt gatccgggaa aagaagtctg cacgccgacg atgcatcatc tgcccggata
      601 cgacaacatg aacatcgata tcgatggcga taactactgt ggtcagttcg ccatgagcac
      661 cctgctcaac ggtatgggca ttcagaccga tcctcaaaag gtctacaaag acaccaaccc
      721 ggccggcatc tttacggctc ctccggtcat cgtcgaatac ctgcgctcga agggcgtcga
      781 cgcccgcatg aagaatcgcg cctcggtcag cgatatcacg aagcgcatcg atcagggcaa
      841 accggtcatg gtgcttgttg attctggcga cggaaccccc cactggatct gcatctacgg
      901 ctacgacacc gatgccagcg gcaaggtcac ctctgtgcgc atgcgcgatt cctactgggg
      961 caccgatggt ccgcacacga tgccgattgc cgatttcgag aaagcctggc gcaaaccttt
     1021 tggtaaaggc attctcggag atctgatgtc ctattccaat gtgctgatcg acaataacgg
     1081 cacgatgaac cccagcaatc ctctgtttcc gggcacgttt gggacggcca cggaagacaa
     1141 catcgcgggt gcgatcaacg atgtcgtgac ggggtggcag aatcataatg tggcgcaggt
     1201 tgtcggcggc gtgaccaaag ccattgtcgg tctgcctggg gctatcaccg gtgtggcttc
     1261 gaactttctc a
//
LOCUS       DAORHD010001553         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_153809_length_1269_cov_3.271829, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001553 DAORHD010000000
VERSION     DAORHD010001553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153809_length_1269_cov_3.271829"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1269
                     /gene="pepD"
                     /locus_tag="PKO06_23420"
     CDS             <1..>1269
                     /gene="pepD"
                     /locus_tag="PKO06_23420"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HNV72679.1"
                     /translation="IPRLSGQEGQVRHWISDLARQRQLTWKQDQVGNLTVFVPATPGR
                     ESYPEMILHAHMDMVGDKAADSDHDFSRDPIRCQRDGDWLKAQGTTLGADNGIGLSAM
                     LALLDEPHLQHGPLRLLFTVDEEGGFTGADGIDPAFLAAPRLINLDSEEHREFNIGCA
                     GGVTHRTEVPISLVPVSHDCIPLRISISGLSGGHSGVEIHHGRANAIRLLARLLLVCQ
                     RDGELRLETMTAGTFDTTIPRQAEAVVWVRTTAADSLQKRLAAVSTSLYRDYSATDQS
                     LKITITRTSGDRSRAVWSDQAAQRLPTFLLAIPAGVLVMSETFADQVETSANPGVVTQ
                     EANRLVLLCHLQSSSSYALHYLGKRVTRLAQHVGGICQEVTPYSPWQPSPDSPLLQQA
                     IATHERLFQQPPRLKVVHAGLECGSFARINP"
BASE COUNT          255 a          396 c          364 g          254 t
ORIGIN      
        1 cattccgcgg ctgtccggcc aggaaggaca ggtacgacac tggatcagcg atctggcccg
       61 acagcgtcaa ctgacctgga aacaggatca ggtgggcaac ctcaccgtgt ttgtcccagc
      121 cacgcccgga cgagaaagct atccggagat gattctacac gctcacatgg acatggtcgg
      181 cgacaaagca gccgattcag atcatgattt ttcgcgcgac ccgatacgct gtcagcgcga
      241 tggcgactgg ctgaaagctc agggaacaac cctcggtgcc gacaacggga tcggtctttc
      301 ggcgatgctg gctcttctcg atgaacccca tctgcaacac ggtcctctcc gcctgttgtt
      361 cacggtcgat gaagagggcg gcttcaccgg tgccgacggg atcgacccgg catttctcgc
      421 cgctccacgg ctgatcaacc tggacagtga agagcatcgc gagttcaaca tcggctgcgc
      481 gggtggcgtg acgcaccgca ctgaggtccc catctcgctc gtcccggtgt cacacgactg
      541 tattccgctt cgtatcagca tctcaggatt gtccggggga cattcaggcg tagagattca
      601 ccatggtcgc gccaatgcga ttcgcctgct cgccaggctc ctgctggtgt gtcaacggga
      661 cggcgaacta cgtcttgaaa ccatgactgc cggaactttc gataccacga ttccccggca
      721 ggccgaggcc gtggtctggg tgagaaccac ggcagcggac tccctgcaga aacgactcgc
      781 agccgtgagt acaagtctgt atcgagatta ttctgcaacc gatcagagtt taaagattac
      841 gattacccga acatcgggtg atcgttcccg ggccgtctgg agcgaccagg ccgcgcagcg
      901 actgccgaca tttctgcttg cgattccggc gggtgtcctg gtcatgagcg agacatttgc
      961 ggaccaggtg gaaacctcgg ccaacccggg tgtcgtgact caggaagcga accggctggt
     1021 cctgctgtgc catttgcaga gttcatcgtc gtatgctctg cactatctcg gcaaacgtgt
     1081 gacccgactg gcccagcacg tgggtggcat ctgccaggaa gtcaccccgt attcgccctg
     1141 gcaaccatcc cccgattccc ctctgctgca gcaggccatc gcgacacacg agcgtctctt
     1201 tcagcaaccc ccgcgactga aagtcgtgca tgccggtctg gaatgcggtt cgttcgcacg
     1261 catcaaccc
//
LOCUS       DAORHD010001554         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_153839_length_1269_cov_2.827018, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001554 DAORHD010000000
VERSION     DAORHD010001554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153839_length_1269_cov_2.827018"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKO06_23425"
     CDS             complement(<1..406)
                     /locus_tag="PKO06_23425"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNV72680.1"
                     /translation="MLHKLESMNLLFQQLTEKLSDPALINNQPEYQKTAKRRAELEPL
                     HRAFTEYQELLTRKKGSEEILATETDPELKGMAEEEVKTLQPEIERLEKQLRFMLIPK
                     DAQDSCNTIVEIRAGTGGEEAALFAAELFRMYT"
     gene            complement(410..1225)
                     /locus_tag="PKO06_23430"
     CDS             complement(410..1225)
                     /locus_tag="PKO06_23430"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018249997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNV72681.1"
                     /translation="MKQRIQEAVTKIRQSVQETPDIAVVLGSGLGAFADELKNAVRVE
                     YHEVPHFPRSTVKGHAGKLVFGTLHGKKLVVMQGRFHYYEGYPLSDVTFPIRVMHALG
                     AKDLIITNASGGLNRNFRLGDLMIMSDHINFIGENPLRGPNDETVGPRFPPMQGAYDT
                     GLINIAEEVGRKLGAPIQKGVYLAVSGPSYETAAELRAFRTLGADAVGMSTVPEVIVA
                     KHAGVKRVLGISCITDMATGEGHEDVNHELVIAAAAAAQPYFLGLLRGVLERM"
BASE COUNT          237 a          356 c          369 g          307 t
ORIGIN      
        1 tcgtatacat gcgaaacaac tcggcggcga acagtgcggc ttcttcgcct cccgtccccg
       61 cgcgaatctc gacgatcgtg ttgcaggagt cctgggcgtc tttcggaata agcatgaagc
      121 gaagctgctt ttcgaggcgc tcgatctcag gctgcagcgt tttgacttcc tcttccgcca
      181 tgcccttcag ttcagggtcg gtttcggtcg cgagaatctc ttctgagcct ttcttgcggg
      241 tgagtagttc ctggtattcg gtgaaagcgc ggtgcagagg ctcgagctcg gctcgccgtt
      301 tggcggtctt ctgatactcc ggctgattgt tgatcagggc cggatcagac agtttttcgg
      361 tcaactgctg aaacagcaga ttcatgcttt cgagtttgtg cagcatcgct cacatccttt
      421 cgagcactcc tcgcagcagc ccgaggaaat acggttgcgc ggcggcggcg gcggcgatga
      481 ccagttcatg gttgacgtct tcgtgccctt cgccggtcgc catgtcggta atgcaactga
      541 tgcccaagac gcgcttgacg cctgcatgtt tggcgacgat cacttccgga accgttgaca
      601 tgccgacggc atcggcaccg agggtgcgga acgcccgcag ctccgcggct gtttcataac
      661 tgggcccgga gacggccaga tacacgcctt tctgaatcgg cgctccgagt ttgcgaccca
      721 cttcctcggc gatattgatc aatccggtgt catacgcccc ctgcatggga ggaaaacgtg
      781 gcccgaccgt ttcgtcgttc ggcccgcgca agggattctc gccgatgaag ttgatgtgat
      841 cagacatgat catcaggtca ccgagacgaa agttgcgatt caaaccaccc gaagcgttgg
      901 taatgatcag atctttggcc ccgagtgcgt gcatgacgcg aattggaaaa gtcacgtcag
      961 agagtgggta tccctcgtag tagtgaaagc ggccctgcat gaccacgagt ttcttcccgt
     1021 ggagtgtccc gaataccagt ttgccggcat ggcctttcac ggttgaacgt ggaaaatgcg
     1081 gcacctcgtg atactcgacc cgcacggcgt tcttcagttc atcggcaaat gctcccaaac
     1141 cggaaccgag aaccacggcg atgtcgggcg tttcctgaac ggactgacgg atcttggtaa
     1201 cagcttcctg aatgcgttgt ttcatttgtt cctgcctggt tctgtagttt ctggtgccgt
     1261 gtgcgggtg
//
LOCUS       DAORHD010001555         1266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_154429_length_1266_cov_3.654831, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001555 DAORHD010000000
VERSION     DAORHD010001555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154429_length_1266_cov_3.654831"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKO06_23435"
     CDS             complement(<1..496)
                     /locus_tag="PKO06_23435"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV72682.1"
                     /translation="MSTDPRESLAVFSDKLGFLLGSGVPLLVALETIALETSDPVMAD
                     ATRQVVRHIGAGSSFHDALAAIPAVFSTSYLSMAQAGENQGTLDIMMPKIARGIRDGL
                     IPFIGLPESSPAAPSDGDPRHAFEQELFTLLEEAVERKASHLLLIPLAEGMRVRFRID
                     GQLAD"
     gene            complement(584..1084)
                     /locus_tag="PKO06_23440"
     CDS             complement(584..1084)
                     /locus_tag="PKO06_23440"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV72683.1"
                     /translation="MTTDACLSEQDLLDLFYGESETPADVRRHLDACDSCRASLAALE
                     RQLTALSDPLPHGGERALAGALKTLGLQSSSGRDVPSAATSIPMISTLSNDILTLSEV
                     AAYLRVDLAQVQSLLPDLPHLNVGGAIRVRRSSLEAFLKRMESESTRRPMTEAVAPSR
                     RRFDLI"
     gene            complement(1074..>1266)
                     /locus_tag="PKO06_23445"
     CDS             complement(1074..>1266)
                     /locus_tag="PKO06_23445"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HNV72684.1"
                     /translation="HLMRTALAQLSEAIREAFLLKHFAELTFVEVAEIQGVPVATAKS
                     RVLFAIRKLRELIGDDHDD"
BASE COUNT          257 a          339 c          440 g          230 t
ORIGIN      
        1 cgtccgcgag ttgaccgtcg atgcgaaagc gaacccgcat accctcggcc agcggaatca
       61 gaagcaggtg agaagccttg cgctcaacgg cttcctcgag cagggtgaag agttcctgtt
      121 caaacgcgtg acgcgggtca ccgtcggaag gcgcggccgg cgacgactcc ggtagaccga
      181 tgaaggggat caggccatcg cggatgccac gggcgatctt gggcatcatg atgtcgaggg
      241 ttccctggtt ctcgccggcc tgtgccatgc ttagatacga ggttgagaag acggcgggaa
      301 tggcggcgag ggcgtcgtga aaactactgc cggccccgat gtggcgtacg acttggcgcg
      361 tcgcatcggc catgaccggg tcggaagttt cgagagcgat ggtttcgaga gcgaccagca
      421 ggggaacgcc ggagccgaga aggaatccga gtttgtcgga aaacacggcg agtgattctc
      481 gaggatcggt ggacatggca agcctcctgg atggttgatg atgggcggtc tggtcagatt
      541 tcggtgaaca atcgccggcg cgctcggcga gtgggagcag acatcagatc aggtcgaagc
      601 gtcggcgcga gggggccacc gcttcggtca tcgggcgtcg ggtcgattcg gattccatgc
      661 gcttcagaaa agcttcgaga gaagaccggc gaacccggat ggccccgccg acgttcagat
      721 gaggcagatc gggcagcagg ctctggacct gtgccaggtc gacccgcaga tacgcggcga
      781 cctcggacag cgtcaaaatg tcgttggaca aggtgcttat cattggaata ctggttgctg
      841 ctgacggtac atctcttcct gacgatgatt gcagaccaag tgttttcagc gcgcccgcga
      901 gagctcgttc gccgccgtgc ggcagggggt cactcaaggc ggtcagttgc cgttcgagag
      961 ccgcgagcga ggcacgacag gagtcgcagg cgtcgagatg ccgacggacg tcggcgggcg
     1021 tttccgactc gccgtagaag aggtcgagca gatcctgttc ggaaaggcat gcatcagtcg
     1081 tcatgatcgt ctccaatcag ttcgcggagt ttgcgaatgg cgaacaacac gcgggatttg
     1141 gcggtggcaa ccggtactcc ctggatctcg gccacctcga cgaaggtcag ttcggcgaag
     1201 tgcttcagca gaaacgcctc gcgaatcgct tctgacagtt gtgcgagagc cgtgcgcatg
     1261 agatgc
//
LOCUS       DAORHD010001556         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_154880_length_1264_cov_3.043011, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001556 DAORHD010000000
VERSION     DAORHD010001556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154880_length_1264_cov_3.043011"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>1264
                     /locus_tag="PKO06_23450"
     CDS             28..>1264
                     /locus_tag="PKO06_23450"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV72685.1"
                     /translation="MSAKFSLGTTLLVGLPMVVILFQALSQANMVRKAESSVHEQRLE
                     SQVRSLEQGVAARVLAMRNKLLRMMHLPEMVAGIEKGKLEHLLHEANPAGFLQYVYLH
                     VSGQYHVESFGSDSANNKTQLNFVLNALSGTDFRFPHDKLASSVGTIMASGGMYVLKA
                     DQLSSMFDRLEPQQLGSRDYYMFSSFSRAANGSKTAFLSLSMPFASIRDRFLDESRLE
                     VLEGASGTRIFYEGERSAIPQIDQFLETSGGSERTFHSIIRRHERTYLAIGRPLRGLM
                     RRVLILSPMPESISTTQTYGQLAIILFLALIAALYSSSDLIRVFLEPLRSLLIAVRAV
                     DNGNFTVSLAPQSADELGRLSGRFNGMVEGLLQRSRMSGYLRSDLLGDGHEALVQRAE
                     RRKLVVLFAGVRDFSRLESS"
BASE COUNT          245 a          339 c          356 g          324 t
ORIGIN      
        1 ctcggggtca gaaccttgtc ggtgagcttg tcagcgaagt tctccctcgg aaccactttg
       61 cttgtcggac tgccgatggt ggtcattctg tttcaagccc tgtctcaggc gaacatggtt
      121 cgaaaagccg agagttcagt gcacgaacag cgtctcgagt cacaggtcag atctctcgaa
      181 cagggggtcg cggcgcgcgt tctggcaatg cgcaacaaac tgcttcggat gatgcatctg
      241 ccggaaatgg ttgccggtat cgagaagggg aagcttgagc acctgcttca tgaggctaat
      301 ccggcggggt tcctgcagta tgtctatctg catgtttcgg gacagtacca cgtggaatct
      361 tttggcagcg attcggcaaa caacaagact cagctgaact tcgttctcaa tgctctcagc
      421 gggacagatt tcaggtttcc gcacgacaag cttgcgagtt cggtgggaac catcatggct
      481 tctggaggga tgtatgttct caaggctgat caactgagct caatgttcga tcgtctcgag
      541 ccgcaacaac tgggcagtcg ggactattac atgttttctt cattctctcg tgccgccaat
      601 ggctcgaaga ccgcgtttct gagtctttcc atgccgtttg cctcgatccg cgatcgattt
      661 ctggatgaat cgcggttgga ggttctcgag ggggcttcgg gcacgcgcat tttttacgaa
      721 ggcgaacggt ctgccattcc acaaatcgac cagtttctcg agacatcggg cggttcagag
      781 cggacgtttc actccatcat tcgccgtcac gaacgaacct atctcgcgat cggcagacca
      841 cttcgcggat tgatgcgacg ggttctgata ctgtcaccga tgcctgaaag catctcaacc
      901 acgcagacgt atgggcaatt ggccattatc ctgtttctcg cccttattgc tgcactgtat
      961 tcctcatcgg atctcattcg cgtgtttctc gaacccttac gctcgttgct gatagccgtg
     1021 cgtgcggtcg ataacggaaa ctttaccgtc agtctcgcgc cacagtcggc ggacgaattg
     1081 gggagactgt caggacgctt caacggcatg gtggaaggtt tgctgcaacg ctcgcgcatg
     1141 agcggttacc ttcgctctga ccttctgggt gatggtcatg aggctctcgt gcaacgcgcc
     1201 gagcgtcgca agcttgtcgt gctgtttgcc ggggtgcgtg atttttcgcg cctcgaaagc
     1261 tcgc
//
LOCUS       DAORHD010001557         1264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_154923_length_1264_cov_2.393714, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001557 DAORHD010000000
VERSION     DAORHD010001557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154923_length_1264_cov_2.393714"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..>1264)
                     /locus_tag="PKO06_23455"
     CDS             complement(40..>1264)
                     /locus_tag="PKO06_23455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNV72686.1"
                     /translation="LLTKGQEVIVQITKEPIGTKGPRVTTDVSLPGRFSVLMPYSNHV
                     GVSRKILGWSEKRRLKALAARLRPENCGIIVRTVAEGQGEDDLTRDINDLKRTWERVH
                     RRAKRMKAPALIHSELGMTTSLMRDLLSEDVERVVVDSKSVYNDIIKYLKGTAPQLRD
                     RVLLYDQKEPVFDAYGIEQEIANATNRRVPLRKGGFLVIDHTEAMVTIDVNTGRYVGR
                     NNQEQTIVNINLEAGQEIARQLRLRDIGGIIAIDFIDMAIEANWKKVWEEFNKALRHD
                     RSRINVAERLSEFGVLEMTRQRVRPSLLYTFSEPCPLCKGSGRVQSPDTTVTKLERWL
                     KRLRAAGGEKKLVLEVHPTVGSYLRENDQRILKALRRTTGAQIILEDNPSLEIDAYRF
                     SSLKTAEDLTAKYEA"
BASE COUNT          241 a          383 c          318 g          322 t
ORIGIN      
        1 gaaacagact ttttaagtta ccatcagttg gatcggctgt caagcctcat atttcgccgt
       61 cagatcctcc gctgtcttca aactggagaa ccgataggcg tcgatttcca gcgaagggtt
      121 gtcttcaaga atgatctgag cacccgtggt tcgccgcagt gctttgagaa tcctctggtc
      181 gttttcacgc agatacgagc caaccgtagg atgaacttcg agaaccagtt tcttctcacc
      241 cccggcggct cgcagcctct tcagccaccg ctcgagtttc gtcaccgtag tatcagggct
      301 ctgtacgcga ccagacccct tgcagagcgg acacggttcg ctgaaggtgt atagtaggct
      361 cggacggacc cgctgccgag tcatctcgag aacaccgaac tcgctgagcc gttccgcaac
      421 attgattcgc gaccggtcgt gccgcagcgc cttgttgaac tcttcccaca cctttttcca
      481 gtttgcttca atcgccatgt cgatgaagtc gatggcgatg atgccgccga tgtcgcggag
      541 acgaagctgc cgcgcgattt cctgccctgc ttcgagattg atgttgacga tcgtctgttc
      601 ctgattattc cgccccacat acctccctgt gttcacatcg atcgtaacca tcgcctcggt
      661 gtgatctatc acaagaaacc cgccttttcg cagcggcacg cggcgattcg tcgcgttcgc
      721 gatttcctgt tcaatgccat atgcatcgaa cacgggctcc ttttgatcat agagaagcac
      781 tctatcacgc aactgggggg cggtgccttt caggtatttg atgatgtcgt tgtacacgct
      841 cttcgagtcc accacaaccc gttccacgtc ttcgctgaga aggtcgcgca tcagactcgt
      901 cgtcattccg agttctgagt gaatcagcgc gggcgccttc attcgtttcg cgcggcgatg
      961 gacccgttcc catgtgcgct tcagatcatt gatatcccgc gtcaggtcgt cttcgccctg
     1021 tccctctgca accgttcgaa caatgattcc gcagttttcc ggacgaaggc gcgctgccag
     1081 cgctttcagc ctccgttttt ccgaccagcc gagaattttg cgcgaaacac ccacgtggtt
     1141 ggaatacggc atcagtacgg agaaacgtcc cggcaacgat acgtcggttg tgacacgcgg
     1201 cccttttgtg ccgattggct ctttcgtgat ctgcacgatc acctcctgcc cttttgtcag
     1261 gagt
//
LOCUS       DAORHD010001558         1263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_155014_length_1263_cov_3.791391, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001558 DAORHD010000000
VERSION     DAORHD010001558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155014_length_1263_cov_3.791391"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..>1263
                     /locus_tag="PKO06_23460"
     CDS             20..>1263
                     /locus_tag="PKO06_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72687.1"
                     /translation="MLIIFFRSPTQPVERALTEIVAQVNRQNRNLRLALLHDGEDPGN
                     VVRQMIGSDTLPADFWASLLQHPTETRWFGERLLSKRILMPSVWLIGTTRMTESGRAG
                     VRKSAPAILVFLGLGLFVLLIGLVQPWFSRSVRFKLLAAFIYSSLMPLAIMGVTARSF
                     FLEHRRMLEDVTHDQVEKAIIGFDRSFHERLKSVAWDLNHRYFSEPNALMASFSHFVT
                     NINSCQEQFRFDSCVVFDNRGQAAFEWHRAGQPPLAGKLIESLAQASRLAIERANPQE
                     MLSRLRKSGASDPPEAGQRMSINESLSAKPMINMLEFGRVKFFFALYPLGQHPDRLTH
                     ISFWFWDKENMQYQYLANNLSRINGILGNGHLLAWNHLDPTVFHPRDFPRLAAVRSIL
                     PRVNNRTRSFRGTLTRDGQTWLV"
BASE COUNT          245 a          380 c          339 g          299 t
ORIGIN      
        1 cgcgcctcat tcacgcggca tgctgatcat cttcttccgg tcgccaactc aacccgttga
       61 gcgagctctc accgagattg tcgcgcaggt caatcgccag aaccggaacc tcagactcgc
      121 cctgcttcat gacggtgaag accctggcaa tgtggttcgc cagatgatcg gaagcgacac
      181 cctgcccgct gacttctggg cctcgctgct gcaacatccg accgagaccc ggtggttcgg
      241 ggaacgtctg ctgtcgaagc gtattctcat gccgtccgtc tggcttatcg ggacgacacg
      301 aatgacggaa tcaggtcgtg cgggtgtcag aaagtcagcc ccggcgattc tggtgtttct
      361 aggactcgga ctctttgtgc tgttgattgg gttggtacag ccctggtttt cacggtcggt
      421 tcgattcaag ctgctggcgg cattcatcta ttcttcgctc atgccgcttg cgatcatggg
      481 cgtcacggca cgctccttct ttctcgaaca tcgccgcatg cttgaagatg tcactcacga
      541 tcaggtggaa aaggcgatca tcggtttcga tcgttccttc catgagaggc tgaaatccgt
      601 cgcctgggat ctcaatcacc ggtacttttc cgagccgaat gctctcatgg ccagcttttc
      661 ccactttgtc acgaacatca attcctgtca ggagcagttc cgcttcgatt cgtgcgtggt
      721 ctttgacaat cgcggacaag cggcattcga gtggcatcga gccggacaac caccattggc
      781 cggcaagctg atcgagtctc tcgcgcaggc ttcgcgtctg gccatcgagc gggcgaatcc
      841 gcaggaaatg ctcagcaggc tgagaaaatc cggtgcgtct gacccccccg aagccggaca
      901 gcggatgtcc atcaacgaaa gtctgtccgc gaaaccgatg atcaacatgc tggaattcgg
      961 gcgcgtgaag ttcttttttg ccttgtaccc actcgggcag catccggatc ggctgactca
     1021 catatcgttt tggttctggg acaaggaaaa catgcagtac cagtatctcg cgaacaatct
     1081 ttcgcgaatc aacggtattc tcggcaacgg ccatctcctt gcctggaatc atctcgaccc
     1141 gaccgtgttt caccccagag attttccgcg cctggcggcc gttcgttcca ttcttccccg
     1201 ggtgaacaat cgaacccgca gttttcgcgg cacgctcacg cgcgacgggc aaacctggct
     1261 ggt
//
LOCUS       DAORHD010001559         1261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_155501_length_1261_cov_2.518242, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001559 DAORHD010000000
VERSION     DAORHD010001559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155501_length_1261_cov_2.518242"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKO06_23465"
     CDS             complement(<1..238)
                     /locus_tag="PKO06_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72688.1"
                     /translation="MNDDPLKSRNPDLSSFSQRVDLYIKNKNHIIDVLQEENDRLRQQ
                     LAQLTADAEKTPAGAAVSAPAIPAEITAELQQLRQ"
     gene            complement(335..1129)
                     /locus_tag="PKO06_23470"
     CDS             complement(335..1129)
                     /locus_tag="PKO06_23470"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNV72689.1"
                     /translation="MKFLTLSDKGSVRPNNEDYTEAVQVKWTGPLGQPDVLTALFLAD
                     GMGGAAAGEFASMLAVRTSKDNLLKALFFRPPEELLHVDLQKFLSDSCIQANTAIFAK
                     ATENKEMEGMGTTLVSAVIYRDILSIVHVGDSRAYLYREGKLQAITRDHSLVQELIDQ
                     GKITHEQAKIHPNRNIITRALGVSPNVEPEGRRIPLYTGDIIVICSDGLCGYVEDSTI
                     QHNIESAEPGPKFDYRAVAENLIKVAIMAGGYDNVSVAVYQHVYLP"
BASE COUNT          250 a          323 c          402 g          286 t
ORIGIN      
        1 gctgccgcaa ctgctgcagt tcggcggtga tctctgccgg aatggcggga gcggaaacgg
       61 cggcaccggc aggagtcttc tcggcatcgg ccgtgagctg ggccagttgc tgtcgtaacc
      121 ggtcgttctc ttcctggagg acgtcgatga tgtgattctt gtttttgatg tacaggtcga
      181 cgcgctgcga aaaggacgag agatccgggt tccggctctt cagcggatca tcattcatag
      241 atgtcagaat aacacacggg attgggctcg taaactggaa ttgcgtgcat ttctcgggag
      301 gtcttcaggt gctcggcaag cgaaggacag gttgtcaggg caggtacacg tgctgataca
      361 ccgcgaccga cacgttatca tacccgccgg ccatgatggc gactttgatc aggttttccg
      421 ccacggcgcg gtaatcgaat ttcggcccgg gttcggcgct ctcgatgttg tgctgaatcg
      481 tggagtcttc cacatagccg cacaggccgt cggagcagat gacgatgatg tcgcccgtgt
      541 acagcggaat acgccggcct tcgggttcga cattcggaga gacacccagg gcacgggtga
      601 tgatgttgcg attcgggtga atcttggcct gctcgtgggt gattttgccc tgatcgatca
      661 gttcttgtac cagcgagtgg tcgcgagtaa tggcttgcag cttgccttcg cggtaaagat
      721 aagcccgact gtcgccgaca tggacgatcg agagaatgtc gcggtagatc acggccgaga
      781 cgagcgtggt gcccatccct tccatttcct tgttttcggt ggcctttgcg aagatcgctg
      841 tattagcctg aatgcacgag tcagagagaa acttttgcag atcgacgtgc agcagctctt
      901 cggggggacg gaaaaacagc gctttcagga gattgtcttt cgacgtgcgc acggccagca
      961 tcgaggcgaa ctcgccggca gcggcacccc ccatgccgtc ggccagaaac agagccgtca
     1021 gcacatcggg ctggccgagg gggccggtcc acttgacctg aaccgcttcc gtataatctt
     1081 cgttgttagg gcgtacagag cctttgtcgc tgagtgtgag aaatttcatg gtgtcctccc
     1141 gcaagactca cgcattttac ctgccgtcct ctgattcgtc aacgagatcg ggaagagaat
     1201 cgcttctaat gagagggtga cgacgattcc ggtggaggtg gggaggatga ggatgggacg
     1261 g
//
LOCUS       DAORHD010001560         1260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_155709_length_1260_cov_2.697095, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001560 DAORHD010000000
VERSION     DAORHD010001560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155709_length_1260_cov_2.697095"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..457
                     /locus_tag="PKO06_23475"
     CDS             158..457
                     /locus_tag="PKO06_23475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72690.1"
                     /translation="MKNLSRASIETFFLGLIYLVGLRLLATISIMDLLLAPGGATSVV
                     TLGIAALFLLFRLFILLCLPGFFLARWYWLSYPAACEKLTTSSSSLKDTSDGNLS"
     gene            441..>1260
                     /locus_tag="PKO06_23480"
     CDS             441..>1260
                     /locus_tag="PKO06_23480"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV72691.1"
                     /translation="METYRSLQARLGTWKARLLVATFLGLWLLPAFIRSSTEWAERLS
                     LDAAQMALAPFRSPDPRLLMINIDTATLQAIPHRWPWPRNEYHRLCQQLLSADPRFLV
                     IDILFQHLEGATASTGDLALAELFRQTRRVGLISVVEETPSPEGLQLRHFRNDKLFRE
                     AATLEGFVWGQYDVDHTIRSVTLADERLGAQSCAVQTARHLNPQLPLPQIDRQGLIRG
                     QLAFLRGQGGIPSVSALDVLEGRVASETLKDRVLFLGVTADILHDWHKSPLGVIS"
BASE COUNT          216 a          409 c          344 g          291 t
ORIGIN      
        1 ttcatcgtga tcgcgaagtg ttccgcggaa tctatcggca actcacgctg ttcggcattc
       61 ccctcgacag cgacggtcga cgcagctatg ctcttggcgg accgctcggc aatgctctgg
      121 tgctggcctt gctcaccgca cgtgaggttt cctccccgtg aaaaaccttt cgagagcttc
      181 catcgaaaca ttctttctcg gactcatcta tctcgtcggt ctgcgcctgc tggcgacgat
      241 ctcgatcatg gatctcctgc tggctccggg tggggcgacc tccgtcgtta ccctgggaat
      301 cgcggccctg ttcctgctct ttcgcctgtt catcctcctc tgtctgccag gattttttct
      361 ggcccgctgg tactggttgt cctaccccgc agcatgcgaa aaactgacca catcttcttc
      421 ttcgctgaaa gatacatctg atggaaacct atcgtagtct tcaagcccgt ctgggcacct
      481 ggaaagcacg tctgctggtc gcgaccttcc tcggcctgtg gctgctgccc gccttcattc
      541 ggtcgagcac cgaatgggcg gaacgcttgt cgctggatgc agctcagatg gctctcgcgc
      601 cgtttcggtc tcccgacccg cgactgctga tgatcaacat cgacaccgcg acgctccagg
      661 ccattcctca tcgctggccc tggccgcgca acgaatatca ccgactgtgc cagcagctgc
      721 tgtccgccga tccacgcttt ctggtcatcg acattctgtt tcagcatctg gaaggagcaa
      781 ccgccagcac gggagatctg gccctcgcgg aactgtttcg acaaacccgg cgtgtcggtc
      841 tgatctccgt ggtcgaagaa actccaagcc ccgaaggctt gcagctccgg cattttcgta
      901 acgacaagtt gtttcgcgaa gcggcaacgc tcgagggttt cgtctggggc cagtatgacg
      961 tcgatcacac cattcggtct gtcaccctcg ccgacgaacg gcttggcgcg cagagttgcg
     1021 ccgtgcagac ggctcgacat ctcaacccgc aactgccgct gccacagatt gaccgacagg
     1081 gactgattcg cggacagttg gcctttctgc gcggccaggg cggcattcct tcggtatccg
     1141 ctctcgacgt gctcgagggt cgagtggcca gcgaaaccct gaaagaccgg gtcctgtttc
     1201 tgggcgtgac agcggacatc ctgcacgact ggcacaaaag ccctctgggg gtcatttccg
//
LOCUS       DAORHD010001561         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_155944_length_1259_cov_2.410299, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001561 DAORHD010000000
VERSION     DAORHD010001561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155944_length_1259_cov_2.410299"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1259
                     /locus_tag="PKO06_23485"
     CDS             <1..>1259
                     /locus_tag="PKO06_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72692.1"
                     /translation="QVTLQEHVHAEDRAVLLSEFQALATGSKQTLELELRLKSPKHGD
                     IWALARGWAERDEQGRVVRIQGTTIDITRIKRAEAEKRLLESQVLHAQKLESLGVLAG
                     GIAHDFNNLLMSILGNADMALATLSAVSPAREHLEQIQLTSKRAADLTRQMLAYPGKG
                     RFVVEPLKLKDIVEEMVHLLQITISKKAVLKLTFAEDAPAIEADGTQVRQVIMNLITN
                     ASEAVGDKSGIISLSTGVQQCDRQYLDLTALGADLPEGDYAFVEVSDTGCGMSREVMD
                     RIFEPFFTTKFTGRGLGMAAVLGIVRGHRGTIKVYSEVGKGTSFKVLFPKVKGSIETK
                     PKTVAVDQNWMGKGIVLLVDDEEMVLRTGRRMLERLGFQVITARDGREALQIFNERHA
                     DIVCTVMDLTMPHLNGEEAFREIRLKH"
BASE COUNT          286 a          348 c          370 g          255 t
ORIGIN      
        1 cccaggttac gctgcaggaa catgtgcatg cagaagaccg cgctgtgctt ctttcagagt
       61 ttcaggctct ggcaaccggc agcaaacaga cgctcgagct cgaacttcgg ctcaagtcgc
      121 ccaaacacgg tgatatctgg gcgctggccc gtgggtgggc cgaacgagac gagcaggggc
      181 gggtggtgcg cattcagggc accaccatcg atatcacccg catcaaacgg gctgaagccg
      241 aaaaacgcct gctcgaaagt caggtgcttc atgcacagaa actcgaaagc ctcggggtac
      301 tggccggtgg catagcgcat gacttcaata acctgctcat gtcgattctg ggaaacgccg
      361 acatggctct ggcgacgctg tctgccgtct caccggcccg tgaacacctc gaacagatcc
      421 agctcaccag caaacgtgca gccgatctga cccggcagat gctggcgtat ccaggcaaag
      481 ggcgattcgt cgtcgaaccg ttgaagctga aagacatcgt cgaagagatg gtgcatctgc
      541 tgcagatcac catttcgaaa aaagccgtgc tgaagctgac ctttgccgaa gatgcgccgg
      601 cgatcgaggc tgacggcacc caggtgcggc aggtcatcat gaatctgatc accaatgcgt
      661 ccgaggccgt cggagacaag agcggcatta tcagcctctc aacgggtgta caacagtgcg
      721 atcgccaata tcttgatctg acggctctcg gcgcggatct tcccgaaggc gattatgcgt
      781 tcgttgaagt ctctgatacg ggctgtggca tgagtcgcga ggtcatggac cgcatttttg
      841 aacctttctt caccacgaag ttcaccggcc gtggcttggg catggctgcc gttctcggca
      901 tcgttcgagg tcatcgcggc acgatcaagg tttacagcga ggtcggcaaa ggcacctcct
      961 tcaaggtgct gtttccgaag gtcaagggtt cgattgagac gaagcccaaa acggttgccg
     1021 tcgatcagaa ctggatggga aaggggatcg tgctgctcgt cgatgatgaa gaaatggttc
     1081 tgagaacggg gcgtcgcatg ctcgaacgcc ttggctttca ggtcatcacc gctcgagatg
     1141 gacgcgaggc tctgcagatt ttcaacgaac gtcatgccga catcgtctgc accgtcatgg
     1201 atctcaccat gccgcatctc aatggcgaag aggcttttcg cgaaatccga ctgaagcat
//
LOCUS       DAORHD010001562         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_156623_length_1256_cov_1.924230, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001562 DAORHD010000000
VERSION     DAORHD010001562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156623_length_1256_cov_1.924230"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PKO06_23490"
     CDS             complement(<1..660)
                     /locus_tag="PKO06_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV72693.1"
                     /translation="MRINQNVLAIRTHGQITQTTSRLDKSVEKLSSGLRINRAADDAA
                     GLAISEKLRRQIRGLSRAILNAQDGISMIQTGEGALNESQSILQRMRELAIQSSNDTL
                     TSNDRLEIQKEVNQLRDDLNRIASNTEFNTKKLLDGSQTALFSTSSTAVKGMIVGYGQ
                     NGGDAAVSLALVSGGVSQMQRSQIFSVRGQESALAKGSTQLQSIAQFYDANGVFTLQS
                     SQ"
     gene            complement(1167..>1256)
                     /locus_tag="PKO06_23495"
     CDS             complement(1167..>1256)
                     /locus_tag="PKO06_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV72694.1"
                     /translation="TRNQILGQSGMAMLAQANTLPQSVLQLLQ"
BASE COUNT          237 a          353 c          347 g          319 t
ORIGIN      
        1 ctgcgaactc tgcagcgtga agacaccgtt ggcatcatag aactgggcga tgctctgcag
       61 ctgggtactt cctttcgcca gcgccgattc ctgacctcga acggaaaaga tctgcgaccg
      121 ctgcatctgc gagacgccgc ctgaaaccag ggccagactc accgcggcat cgccgccgtt
      181 ctgtccgtag ccaacgatca tgcccttgac agccgtgctg ctggtgctga agagagcggt
      241 ctgagagcca tccagcaact tcttcgtgtt gaactcggta ttgctggcga ttcggttcag
      301 gtcatcgcgc agctggttca cttccttttg aatctcgagt cggtcattgc tggtcagggt
      361 gtcgttgctg gactgaatcg cgagttcccg catgcgctgc aggatgcttt gcgattcatt
      421 cagggcgcct tcacccgtct gaatcatgga aataccatcc tgagcattga gaatggcgcg
      481 ggaaagacct cgaatctgcc gccggagctt ttcactgata gccaggccgg cagcatcgtc
      541 cgcggcgcgg ttgatacgga gtccggaact cagtttctcg accgatttgt cgagacggct
      601 ggtggtctgt gtgatctgac cgtgagtgcg aatggcgaga acattctgat tgatacgcat
      661 atgattcctc cttgaatctg tgccacgagc atccttgctc taacgggcgt atgcgtgtgg
      721 agtaactggg acctcagcaa agagagtcgc cgcggaatgt gaagattact ggttcggtgt
      781 tcacctcctt actatcttac ttgcctgctt tagggagtat ttttgataga atctccccta
      841 tcttctgtac tttagatcgt cgttcttgat gagaaacacg agagtcgatt ttcagcgatt
      901 ccaggctcag gaagccagat atccgatccg gcttcggtat ttccatcgag acatgctctt
      961 ttttgccgtt tttccgccca ttctttagca tttcgttcat tggtcctgtg tcttgagggg
     1021 gcagagctca ctcgcccggt agcgtcgcag cactctgagt tcgacacgcc cctgctccgt
     1081 caaaccatgc tgcacggact tatcgagctc cgccccggtt ccggggcggc gagtggtgac
     1141 cgtcaccacc cgccgacctc atccggttac tggaggagct gcagcacgct ctgtgggagc
     1201 gtattcgcct gggccagcat cgccatgccc gactgaccaa gaatctgatt gcgtgt
//
LOCUS       DAORHD010001563         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_156709_length_1255_cov_3.406667, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001563 DAORHD010000000
VERSION     DAORHD010001563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156709_length_1255_cov_3.406667"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKO06_23500"
     CDS             <1..608
                     /locus_tag="PKO06_23500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72695.1"
                     /translation="LPAGTGEDALLHDSLRTEIQHLLDEILAYRHLQQAETGKLQMKW
                     SGIHLRGFLDQLVRGCQTQECAQHRSIVIEPPVPEQELLTDDRLLRRILVNLIKNALE
                     ATPTGEAIRVRCRPESEFIRFEVWNPGAIPVVVQYQIFQKSFSTKGMPGRGLGTYSAK
                     LLGERGLRGHLSFNSDALSGTTFTFLHPLNSDKAAPQAQSS"
     gene            complement(729..>1255)
                     /locus_tag="PKO06_23505"
     CDS             complement(729..>1255)
                     /locus_tag="PKO06_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72696.1"
                     /translation="GPQQADSPVFSFFTLPLTAAMPVLRLLRLALQQVTVTTQAETVR
                     LRLPLRVLFAAFSSPLALISLLPIVPMLLAAASRTAKPVVAGVVETLRDGGCSAVRQA
                     VNTACQGYLMDRGASVPVDACPDMLELLAAGCIRADQTCPQGGTFSIRRQHETFVVSC
                     SVHAPERKSDDGAA"
BASE COUNT          265 a          386 c          372 g          232 t
ORIGIN      
        1 ggctaccggc cggcaccggc gaggacgctc tgctgcatga ctcgctgcgc acggaaattc
       61 agcacctgct cgacgagatt cttgcgtatc gccatctgca gcaggccgag acaggcaagc
      121 tgcagatgaa gtggagcggc attcacctgc gcggatttct cgaccagctc gttcgcggtt
      181 gccagaccca ggagtgcgcc cagcatcgct cgatcgtcat cgagccaccg gtacccgagc
      241 aggaactctt gaccgacgac cgtcttctga gacgcattct ggtgaacctg atcaagaacg
      301 cgctagaagc gacgcccacc ggagaggcca ttcgggtacg ttgtcggcca gagtccgagt
      361 tcatacggtt tgaagtctgg aacccaggcg cgattccggt tgtcgttcag tatcagatct
      421 tccagaagtc gttttcgacc aagggcatgc ccggacgcgg acttggcacg tattcggcga
      481 aactgctcgg cgagcgcggt ttgcgcggac acctttcctt caacagcgac gccctgtcag
      541 gtaccacgtt cacgtttctg catccgctca attccgacaa ggctgcacca caagctcagt
      601 cgagctgaga agcaatcgca ggaagacgcg gcggtctctg agacaggttg cacgctgtcg
      661 tgttccctgt gtctgtgcgc gtgtcagtcg ttcgcgcgat cgattgcgca atgcggctgc
      721 agatcatgtt atgccgcacc atcgtcggac ttccgctctg gcgcatgcac cgagcaggaa
      781 accacgaacg tttcgtgctg tcgtcgaatg ctgaatgttc ccccctgcgg gcaggtctga
      841 tcagcgcgaa tacatccggc cgccagcagt tcaagcatat ccgggcaggc atcaaccggt
      901 acacttgcgc cacgatccat gagataccct tgacaggcag tgttgacggc ctgacgtacc
      961 gcggagcaac cgccgtcacg caatgtctcg acgactcccg ccacgaccgg tttcgcggtc
     1021 ctggacgccg ccgccagaag catcggaaca atcggcagca ggctgatcag agccaacggc
     1081 gatgagaatg ccgcaaagag cacgcgtaac ggcaaacgga gccgaaccgt ttccgcctgc
     1141 gtggtcacgg ttacctgctg cagtgccaga cgcagtagac gcagaaccgg cattgcagcg
     1201 gtcagtggca gggtaaaaaa cgagaacacc ggggaatcag cctgttgcgg gccag
//
LOCUS       DAORHD010001564         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_156729_length_1255_cov_2.940833, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001564 DAORHD010000000
VERSION     DAORHD010001564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156729_length_1255_cov_2.940833"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1255
                     /locus_tag="PKO06_23510"
     CDS             <1..>1255
                     /locus_tag="PKO06_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72697.1"
                     /translation="GLQCSTNTIDLGDWRPGDTVATTSITVTNIGNQNLPMVFMQVSS
                     FTSGANWIASDNVKISPNQYIGLMVKGTSSNKSFSVTIPGAQPTGTYVATATFINEKV
                     TVNAIPEPGEASFPIKLTLYITASEGMNLGSTTVILATSTPGAQVATGLIRLRNIGDL
                     GLEKLKIEWTPLTFAGPGTGSLTIASSCLRIVPTSLPVVNKSASTTFSVAVDIPFGVA
                     SSAVGSAFTGVQWIFNDRNGNDIRDAGETSASFTLRLGISRLPAVVVAEELANFGTGV
                     QGAVKTCQISIMNTGNSACSGLKWRVLDQLTDGTNTIASSAITFLPNGIHVASWAFNF
                     PTVTSTTATLTLTIPGVIPAGVYQGRCCLFNDVVTVDNASTTGESYDEFTLRIEVGSG
                     SLRFATDTMDLGAANPYEDSSLRVFS"
BASE COUNT          272 a          373 c          335 g          275 t
ORIGIN      
        1 ggtctgcaat gctcgaccaa caccattgat ctcggagatt ggcgccccgg agataccgtc
       61 gcgaccacct ctatcacggt gacgaacatc ggtaaccaga accttcccat ggtgttcatg
      121 caggtctcgt cgttcaccag cggtgcgaac tggatcgcca gcgacaacgt aaaaatctcg
      181 ccgaatcagt acatcggcct catggtgaag gggacttcga gcaataagtc cttctccgtc
      241 acaattcccg gcgctcaacc gaccggaacc tacgtggcca ctgccacttt catcaatgaa
      301 aaggttaccg tgaacgccat tccagaacct ggcgaagcgt cgtttccgat caagctgacc
      361 ttgtatatta ccgcaagcga aggcatgaat ctcggttcca ccaccgtcat tctggcgacc
      421 agtactccag gagcgcaggt ggcaaccggc ctgatccgcc tgcgcaatat cggcgatctc
      481 ggtcttgaaa aactgaagat cgaatggacc cccctgacgt tcgccgggcc agggaccggc
      541 tcgctgacga tcgcttcaag ctgtcttcgg atcgttccga ccagtcttcc cgtcgtgaat
      601 aaaagtgctt caaccacgtt ctctgtcgcc gtggatattc ccttcggtgt ggcgagcagc
      661 gcggtcggct cggcattcac cggcgtgcag tggattttca acgatcgcaa cggaaacgac
      721 attcgtgacg ccggcgagac gtcagccagt ttcactctgc gactgggtat ttcgcgtctg
      781 cctgcggttg tcgtcgccga agaactggcc aacttcggaa ccggcgtaca gggggcggtg
      841 aaaacctgcc agatcagcat catgaatacc ggaaactctg cctgctcagg gctgaaatgg
      901 cgcgttctcg atcagttaac agatgggaca aacaccatcg ccagttctgc catcaccttt
      961 ctgcccaacg gcattcatgt ggcgagctgg gcgttcaact ttcccaccgt tacctcgacg
     1021 acagcaacat tgacgctgac gatccccggg gttatccccg ccggcgtcta ccaggggagg
     1081 tgttgtctgt tcaacgatgt agtgacggtg gacaatgcgt caaccaccgg cgaatcgtac
     1141 gatgagttca ctctgagaat cgaggtggga agcggaagtt tgcgttttgc cactgacacg
     1201 atggatcttg gagccgctaa tccctacgag gattcttctc tccgcgtctt ttcgg
//
LOCUS       DAORHD010001565         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_156926_length_1254_cov_3.211009, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001565 DAORHD010000000
VERSION     DAORHD010001565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156926_length_1254_cov_3.211009"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..813
                     /locus_tag="PKO06_23515"
     CDS             127..813
                     /locus_tag="PKO06_23515"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HNV72698.1"
                     /translation="MSEQRKTRRILIAAGGSPDQPPPGTAWDLIIAVDHGGDLLRTWN
                     LDADTVIGDLDSLSPEALKHHLDRGAEVVRYSADKNETDLELALRSLPNDPHAEIYLT
                     NLWGGRFDHSIMNLLMMSHFTCKGLLVFPTTDGIGGIMSAGQLAVAAPHHLGTALIAL
                     SSEVTGISSHGVQWPLQKETLRFGESRGVSNRLIRSPWRLQAHTGTLLWLISGVSRAA
                     VEIEWLPAVL"
     gene            complement(758..>1254)
                     /locus_tag="PKO06_23520"
     CDS             complement(758..>1254)
                     /locus_tag="PKO06_23520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72699.1"
                     /translation="DVAGNLSVYPIDGEFLRQNRRCETLRLSEFGTTWGPRVPWVDEW
                     PVEPLGLPPLERLLAYRRIQVPATLVEDIVGRLREAGMEDTVQVVGMETPAVQMGSYL
                     QDLRLMREGHLPMQRVDLLRQDIVNLVVPTYLLPVPESLPDQPEFRELREAIQFRLLH
                     EKPR"
BASE COUNT          281 a          397 c          333 g          243 t
ORIGIN      
        1 gaaaacatcg acggacgcat aacatcgccg ctccagatcc agcagatctg gagcatccgt
       61 gagcgtggcc aaaacgattc cagtggaagt tgattccata agaggaaaaa gtataccttt
      121 gtcatcatga gcgagcaacg gaaaacccgg cgcattctga tcgccgctgg cggttcacca
      181 gaccagccac cccctggtac agcctgggat ctcatcatcg ccgtcgatca cgggggcgac
      241 ctgctgcgca cctggaacct cgacgcggac accgtgatcg gcgatctcga ctcactcagc
      301 ccagaggctc tcaagcatca tctcgaccgt ggagccgagg tggttcgcta ctcggcggac
      361 aagaacgaaa ccgatctcga actcgctctg cgcagcctgc ccaatgaccc gcacgccgag
      421 atctatctga cgaatctctg gggagggcgt ttcgatcaca gcatcatgaa tctgctgatg
      481 atgtcgcatt ttacctgcaa ggggctgctc gtctttccga caaccgacgg catcggcggc
      541 atcatgagcg ccggacagct ggccgtggcg gctccccacc atctgggaac cgcactgatc
      601 gcactttcat ctgaagtcac cggcatctct tcacacggcg ttcagtggcc acttcaaaaa
      661 gagactctcc gttttggtga aagtcgcgga gtcagcaatc gcctgattcg gtcaccctgg
      721 cggctgcagg cgcacaccgg aaccctgctc tggctgatca gcggggtttc tcgtgcagca
      781 gtcgaaattg aatggcttcc cgcagttctc tgaactccgg ttggtcaggg agagattcgg
      841 gaacaggcag cagataggtc ggtaccacca ggttcacgat gtcctgtcgc agcagatcaa
      901 cccgctgcat cggcaagtgc ccctcacgca tcagtcggag atcctgcaga tagctaccca
      961 tctgcacggc cggagtttcc ataccgacaa cttgtacagt atcctccatc cccgcctcgc
     1021 gaagtcttcc cacgatatcc tcgaccaaag tcgcgggcac ctgaattcgg cgatatgcca
     1081 gaagccgctc caaggggggt aaacccaggg gttccaccgg ccattcgtca acccagggca
     1141 ctcgcggtcc ccatgtcgtt ccaaactcag aaagccgcag ggtttcacaa cgtcgattct
     1201 gccgcagaaa ctccccgtcg atgggataga cagaaagatt cccggccacg tctt
//
LOCUS       DAORHD010001566         1254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_156999_length_1254_cov_2.274395, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001566 DAORHD010000000
VERSION     DAORHD010001566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156999_length_1254_cov_2.274395"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..789
                     /locus_tag="PKO06_23525"
     CDS             124..789
                     /locus_tag="PKO06_23525"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV72700.1"
                     /translation="MLIISILGASLSLVLLAPRCPRLHTLIGFLLLGVIAPQFVSLAH
                     IDLIRRLWVSLTAPALPVFILLSTAVVLCLFLPRGYCRLLCPAGHLQDLVSLAVSRNA
                     TERRDSTVKLSDSGHANRPLPIGRLLLWGGMCAFILTPAFPLHHLEVFSALLFRNLGI
                     SGWLLVLALLIGSLSTHRWYCFTLCPLNHLFADFEFFLQSCRRSSSEKENPAVPSEEQ
                     AKP"
     gene            786..>1254
                     /locus_tag="PKO06_23530"
     CDS             786..>1254
                     /locus_tag="PKO06_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72701.1"
                     /translation="MNRRQFLHIASTCLGAGGIAGMLLTDNRGHPLQSSHQAQPSSRE
                     QFDAAEARRTFQHLENAGFTPREALYWERSGANRIRCTLCPTFCELRPYERGHCRVRF
                     GLENRLVTVVYGKPCSVAIDPIEKKPVFHLLPGSTSFSFATAGCLLRCRYCQNW"
BASE COUNT          222 a          391 c          310 g          331 t
ORIGIN      
        1 gcgacagtat ctgtagaggc attctgtcgc actgttcatg aaacactgcg tcgcgcctct
       61 tcccaaggtc aaacggttcc cctggaatcc acttcatccg tacctgttga ttcaactgct
      121 ttcatgctca ttatcagtat tctgggggca agtctctcgc ttgttcttct ggctccccgt
      181 tgtcctcgtc ttcataccct gatcggtttt ctgctcctgg gagtcattgc accacaattt
      241 gtctcgctgg cacacattga tctgatccgc cgtctgtggg tgagtctcac ggcaccggca
      301 ctaccggtgt tcatcctgtt atctacagct gttgtcctct gtcttttcct gccacggggg
      361 tattgccgtc tgttgtgccc ggccggccat cttcaggatc tcgtttcact ggcggtttcg
      421 cgaaacgcaa ccgaaaggcg agattccacc gtgaagctca gcgactctgg ccacgcgaac
      481 aggccacttc caatcgggcg gcttctgctc tggggcggca tgtgtgcctt catcctgact
      541 ccggcctttc cgttgcacca tctggaagtg ttttcggctc tgttgtttcg caacctgggt
      601 atcagcggct ggcttctcgt tctggctctt ttgatcggat ctttgtcaac ccatcgctgg
      661 tactgtttca ctctctgtcc gctcaaccac ctgttcgccg atttcgaatt cttcctgcaa
      721 tcatgtcgca ggtcatcgtc agaaaaagag aaccccgcgg ttccatcaga agagcaggcc
      781 aagccatgaa tcgtcgccag tttcttcata ttgccagcac ctgtctcgga gcgggtggaa
      841 ttgccggcat gctcctgacc gacaatcgcg ggcacccgct tcagagttcc caccaggccc
      901 agccgtcaag ccgcgaacaa ttcgatgcag cagaagcgcg gcgaacgttt caacatctcg
      961 agaacgccgg gttcactccc cgcgaagccc tctactggga aaggagcggg gcgaaccgta
     1021 ttcgctgcac tctctgccca acattctgcg agttgagacc ttatgaacgc ggacactgcc
     1081 gtgtgcgctt tggcctcgaa aaccggttgg ttacggtggt atacggcaaa ccctgctcgg
     1141 tcgccatcga ccccatcgag aaaaaaccgg tattccatct tcttcccggc tcaacctcgt
     1201 tttctttcgc gaccgccggt tgtctgctcc gttgtcgtta ctgccagaac tggc
//
LOCUS       DAORHD010001567         1252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_157328_length_1252_cov_3.519632, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001567 DAORHD010000000
VERSION     DAORHD010001567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157328_length_1252_cov_3.519632"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKO06_23535"
     CDS             <1..837
                     /locus_tag="PKO06_23535"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNV72702.1"
                     /translation="GVVAVSLTIGVCMRLLFPDAESARQVPMAMVEEDTDRTTRQLFL
                     FFAILVCIMLTSTGIFDRPAVRLGQQLGLIDAPVRQQAGDPDSGSASRTGNPEIGSTS
                     ERLAGATSVEANLALPVLAKFLMLGLELAILMLMLKRWFLREEIVGWLKKSWSLFLSI
                     FPMVLIGLFVSGVLAALLPMLKFMTWFRDNTPASNILVSFIGAMSYFGTIVGVNIVAT
                     MNHFGMHIGPCLALLLSGPAVSLPSVLALVPIVGSRKAATYLILVVVCSALTGYIYGT
                     LQ"
     gene            862..>1252
                     /locus_tag="PKO06_23540"
     CDS             862..>1252
                     /locus_tag="PKO06_23540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72703.1"
                     /translation="MNTVETQIEKIQEMMKDGKYDEARPLLEELTSKNPDNPLLLRLL
                     GNTYAYTGFLNKAKKLWRDGCRRFPENVDLIYNLGLAFYLQGNFPRARFLWKKGLKLS
                     PTDGEILFNLGQISRDEGHFRKAIKYWK"
BASE COUNT          259 a          329 c          351 g          313 t
ORIGIN      
        1 ggggtcgtcg cggtttcgct gaccatcggt gtctgcatgc gactgttgtt tccagacgcg
       61 gaatcagcac gtcaggtgcc gatggccatg gtcgaggagg acaccgatcg caccactcgg
      121 caattatttc tcttttttgc gattctcgtc tgcatcatgc ttacctcgac aggcatcttc
      181 gaccgtccgg ctgtgcgcct gggccagcaa ttggggttga ttgacgcgcc cgtcagacag
      241 caggcaggcg atcctgattc tggaagtgct tctcgaacag ggaaccctga gatcggctcg
      301 acgagtgaac ggttggccgg tgcgacatcg gtcgaagcga atctcgccct tccggttctg
      361 gcgaagtttc tgatgctggg gctggaactg gcgattctga tgctgatgct gaagcgttgg
      421 ttcctgcgcg aagagattgt cggttggctc aagaagtcat ggagcctgtt tctcagcatc
      481 tttccgatgg tactcattgg cctgttcgtt tccggggtgc tcgcggctct gcttcccatg
      541 ttgaagttca tgacgtggtt tcgcgacaat acccctgcga gcaacattct tgtcagcttc
      601 atcggcgcca tgagttactt tggaaccatc gtcggcgtga acattgtggc gaccatgaat
      661 catttcggca tgcacatcgg tccgtgcctg gcgctgcttc tttccggacc ggccgtcagc
      721 ctgccgagcg ttctcgccct tgtaccaatc gtcggctcga gaaaggcggc tacgtattta
      781 attttggtgg ttgtctgttc agcgctgaca ggatatattt atggcactct tcagtaagct
      841 cacggaagga atcgataaaa cgtgaacacc gtagaaacac agattgagaa gattcaggaa
      901 atgatgaagg acgggaagta tgacgaagcc cggcctctgc tcgaagaact tacgagcaag
      961 aaccccgata accctttgct gcttcgactg ttggggaata cctacgcata caccggtttt
     1021 ctcaacaagg ccaagaagtt gtggcgtgac gggtgtcgtc ggttcccgga aaacgtcgat
     1081 ctgatctaca acctcggcct tgccttctac ctgcagggca attttcctcg cgcccgtttc
     1141 ctctggaaaa agggcctgaa gctgtcacca accgacggtg agattctgtt caacctcggt
     1201 cagatttccc gagatgaagg gcacttccga aaagcgatca aatactggaa ga
//
LOCUS       DAORHD010001568         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_157843_length_1250_cov_2.454393, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001568 DAORHD010000000
VERSION     DAORHD010001568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157843_length_1250_cov_2.454393"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PKO06_23545"
     CDS             <1..582
                     /locus_tag="PKO06_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNV72704.1"
                     /translation="DYQELTKGIWVDNPLFVIMLGLCPTLATSTSVMNAIGMGIAATF
                     VLIGSNCIISLVRDIVPNNVRIPVYIVVIATFVTMIDKFMAAFTPALSASLGIFIPLI
                     VVNCIILGRAEAFANKNTVWASFLDAIGMGLGFTLALCIIAAFREVLGDGKFAGFTVF
                     RNDPALFMIMAPGGFFVFGLLMALKKHMARKGA"
     gene            585..1163
                     /gene="rsxA"
                     /locus_tag="PKO06_23550"
     CDS             585..1163
                     /gene="rsxA"
                     /locus_tag="PKO06_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005886061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNV72705.1"
                     /translation="MSGSIISIVIIAIFIENFVLNQILGLCPFVGVSNKIDSAVGMGL
                     AVTFVMALAGAVCWAVQFYLLLPFHLEYLQTIAFILVIAALVQFVEMVIRKMAPSLYQ
                     SLGVYLPLITTNCAVLGVALLNLKKGFNLVQSVANSTAAGVGFFFALILMAGIRERLE
                     TADIPLAMRGAPITFITAGLMALAFMGFAGLA"
BASE COUNT          219 a          329 c          356 g          346 t
ORIGIN      
        1 gattatcagg agctgaccaa aggcatctgg gtcgacaacc cgttgtttgt gatcatgctc
       61 gggctctgtc cgacgctcgc gacctcgaca tcggtcatga atgccatcgg catgggtatt
      121 gccgcaacgt ttgtcctgat cggttcaaac tgcatcatct ctctggtgcg cgacatcgtc
      181 ccgaataatg tgcgcattcc ggtctacatc gtggtcattg ccacattcgt cacgatgatc
      241 gacaagttca tggccgcgtt cactccggct ttgtcggcat cgctgggaat cttcattccg
      301 ctgatcgtcg tgaactgcat tattcttggt cgcgcagaag cgtttgccaa caagaatacg
      361 gtgtgggcat cgtttctcga cgcgatcggc atggggttgg gattcactct ggccctgtgc
      421 atcatcgcgg catttcgtga ggttctcggc gatggcaagt tcgctggttt caccgtcttc
      481 aggaacgacc cggcgttgtt catgatcatg gctcccggtg gatttttcgt ctttgggctg
      541 ctcatggcgc tgaagaagca catggcgcgg aagggggcgt aagcatgagt gggagcatca
      601 tcagtatcgt catcatcgcg attttcatcg aaaactttgt tctcaaccag attctcggcc
      661 tgtgtccgtt cgtcggggtc tcgaacaaga ttgattcggc tgtcggcatg gggctggccg
      721 tgaccttcgt gatggctctg gccggggccg tttgctgggc cgtgcagttt tacctgctgt
      781 tgccctttca tctcgagtat ctgcagacca tcgcgttcat tctcgttatt gcggctctgg
      841 tgcagtttgt cgagatggtc attcgaaaga tggcgccttc cttgtaccag tcattgggag
      901 tgtatctccc gctcatcacc acaaactgcg ccgtgctcgg cgtggctctg ctgaacctga
      961 aaaagggttt caatctggta caatccgtcg cgaactcaac cgcagccgga gtcgggttct
     1021 tctttgcgct gattctcatg gcgggtatac gtgagcgtct cgagactgcc gatattccgc
     1081 tggccatgcg gggagcgccc ataaccttca tcaccgcggg cttaatggcg ctggctttca
     1141 tgggctttgc cgggttggcc tgaaaaaata gaggacacaa catatgatgt tcattaatgc
     1201 cattctggtt ctcggggggc tggggtttct cttcggtgca attctcgcgt
//
LOCUS       DAORHD010001569         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_157974_length_1249_cov_3.681742, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001569 DAORHD010000000
VERSION     DAORHD010001569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157974_length_1249_cov_3.681742"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PKO06_23555"
     CDS             complement(<1..921)
                     /locus_tag="PKO06_23555"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNV72706.1"
                     /translation="MKIEITNIDGSVLFEGAITPGKEGVILGNDRSCDVYLPYLAVAQ
                     RHVSIQHASAREAILTDLGSAGAVQVNGRHLQPGFVAHVTIGDKIQLAEGLFLSIRPG
                     ADPEIPAIGQEEGMPTVFPFFIERNERLVKEIFTELRTQLPTSARSVIKSAEDRVSNK
                     VRELSAILEVSFALNSVFNYQRLIEYVLDMAQKVSNTERGAVLLFNEEIDRLETVFLR
                     GGSLKELEKDLKAAATDVLKCFRTGEMVFFPPVNPTQESMRSKRAAASEHAPARAVAL
                     APLRNQSGVIGVLYLDRPYASGVFTPQCHDL"
     gene            1066..>1249
                     /locus_tag="PKO06_23560"
     CDS             1066..>1249
                     /locus_tag="PKO06_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72707.1"
                     /translation="MVFRRDYCLKVLLVLALLAPVALMASEFKQYLRNPDPDRYVQPG
                     YMPVDPKGDAYLIRKID"
BASE COUNT          248 a          379 c          356 g          266 t
ORIGIN      
        1 cagatcgtgg cactgcgggg tgaagacccc cgaggcatac gggcgatcaa gatagagaac
       61 cccgatgacg ccgctctggt ttcgcaacgg tgccagggcc acggcgcgcg ccggggcatg
      121 ctcgctggct gcggcgcgtt tcgaacgcat gctctcctga gtcggattta ccgggggaaa
      181 gaacaccatc tcaccggtac gaaagcattt gagcacgtcg gtggccgccg ccttgagatc
      241 cttctccagt tccttcaggc tgccgccgcg cagaaagacg gtctcgagcc ggtcgatctc
      301 ttcgttgaac agcagcaccg cgccgcgctc ggtgttcgag accttctgcg ccatatcgag
      361 cacatactcg atcagacgct gatagttgaa gaccgaattg agggcgaaac tgacctcgag
      421 aatggccgac agttcgcgaa ccttgttgct gacccgatct tcagcgctct tgatcacgct
      481 gcgagccgag gttggcagtt gggtgcgcag ctcggtgaag atctctttca ccagacgctc
      541 attccgttcg atgaaaaacg gaaacaccgt cggcatccct tcttcctgac cgatggcagg
      601 aatctcggga tcggctccag gacgaatcga gagaaacaat ccctcggcca gctgaatctt
      661 gtcgccgatc gtcacgtggg ccacaaagcc gggctgcagg tgtcgaccat tgacctgcac
      721 ggctcccgcg cttcccagat cggtcagaat ggcctcgcgt gccgaggcat gctgaatgct
      781 cacatgtcgc tgggcaactg cgagatacgg aaggtagaca tcacacgatc ggtcattgcc
      841 cagaatcacg ccttccttgc ccggcgtaat ggcaccctcg aacagcacgg aaccgtcgat
      901 gttggtaatc tcgattttca tggcgtcccc ttccttcgtg tggcattcac tcggtgtatc
      961 tggtggtcag tatagcgcaa ttccctgtct cgttcacctt gacattcctc ttcccagacc
     1021 ccgggggaga tctggtaaaa ttgaccgaaa gggggtaccg aacccatggt ctttcgccgc
     1081 gattattgtc tgaaagtgtt gctggtgctg gctctgttgg ctccggtggc cctcatggcc
     1141 tccgagttca agcagtatct gcgcaacccc gacccggatc gctacgtcca gcccgggtat
     1201 atgccggttg acccgaaggg cgatgcctat ctgatccgca agatcgacc
//
LOCUS       DAORHD010001570         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_158200_length_1248_cov_4.568315, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001570 DAORHD010000000
VERSION     DAORHD010001570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158200_length_1248_cov_4.568315"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1248)
                     /locus_tag="PKO06_23565"
     CDS             complement(<1..>1248)
                     /locus_tag="PKO06_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72708.1"
                     /translation="HKVTFGIYGGTVRDLYLGRRFSTISDFDTIYDSSEPGFPAFRNR
                     VLERARSLKAGIPSPDFHFDLSSLSRPSERQRQYHEEGITATKVGVFCDGTMIDPTGY
                     GVKDLRQRIFRFVPSDKSFIELQNVGRFIRDYVRLPGFRADSATIELMRRSLDRCRKS
                     DTPEGQQTRAAVASCRALPREKDWLTFPALINVFRQDLRWLHNGRSERLSTCYPVNLF
                     LVELFRTVTQADDVKSMQSGFARFDLASFLMAIGLEQEGKLLTDPTLSRDALFDAFQF
                     PGHWPEVPRSISFLEKWTRTVTRYNYRVLFDMMKSEFPADSFEAKVLTWRRDGFLSPG
                     SYRMMNPGTNWREMIESLLDADFNLGTLPREKTMAGLDWFLKVYLPHGETVLKPVSVG
                     QISRSALREIRVGFSPEIRLVSR"
BASE COUNT          314 a          343 c          336 g          255 t
ORIGIN      
        1 gccgagacac gagacgaatt tcaggagaga atccgacgcg gatctcccga agagccgacc
       61 gagaaatctg gccgaccgac accggcttca acaccgtttc accatgcggc agatagactt
      121 tcagaaacca gtcgagtccg gccatcgttt tctcgcgcgg caaggtccca aggttgaaat
      181 ccgcatcgag caggctttca atcatttcgc gccagttggt tccaggattc atcatgcgat
      241 aggatccggg tgacagaaac ccatcgcgcc gccaggtgag caccttcgct tcaaacgaat
      301 cggcggggaa ctccgacttc atcatgtcga acaagacgcg gtaattgtat ctcgtaactg
      361 tccgagtcca tttttcgaga aacgagattg atcgcggcac ttcgggccag tgaccgggaa
      421 actgaaaggc atcgaagaga gcgtcacgcg acagggtcgg atcagtcagc agctttcctt
      481 cctgttccag tccgatggcc atgagaaaac tcgccagatc gaaacgggca aatccagact
      541 gcatgctctt cacgtcatct gcctgagtca ccgtacgaaa cagttcgacc agaaagagat
      601 tcaccgggta acaggtactg agtcgttcgg aacggccgtt gtgcaaccag cggagatcct
      661 ggcgaaagac attaatcaag gctggaaagg tcagccagtc tttctctcgc ggcaaggctc
      721 gacaagaggc taccgcagca cgagtctgct ggccctcagg cgtatcgctt tttcgacatc
      781 ggtcaagtga acgtcgcatc agttcgatcg tcgccgagtc agcgcgaaaa cccggtaagc
      841 gcacgtagtc gcggatgaat cttccgacat tctgcagttc gatgaatgat ttgtccgagg
      901 gaacgaagcg gaatatccgc tgtcgaagat ctttcacccc gtaacctgta ggatcaatca
      961 tggtcccgtc gcaaaacact ccgactttcg tggccgtaat gccttcttcg tgatactggc
     1021 gctgacgttc actcggtcgc gacagcgacg acagatcgaa gtgaaagtcg ggcgacggaa
     1081 tacccgcctt gagactgcgt gccctttcga gcacccggtt gcgaaaagcc ggaaatcccg
     1141 gttccgagga gtcgtagatg gtatcaaaat cggaaatggt tgagaaccga cgacccagat
     1201 acaggtcacg tacagtaccg ccgtagattc caaaagtaac tttgtgtt
//
LOCUS       DAORHD010001571         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_158295_length_1248_cov_2.514669, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001571 DAORHD010000000
VERSION     DAORHD010001571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158295_length_1248_cov_2.514669"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="PKO06_23570"
     CDS             complement(<1..1104)
                     /locus_tag="PKO06_23570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72709.1"
                     /translation="MSVPVPEFVEPLTKKVLTLPRESIASISVLAFHASFHAYRTIIQ
                     GDIPFELWLWSPTRRHLIITTHDNIGFILLCWFGGTWGLLLLRTLLTRHQLALGLRTW
                     LFAFFALIGAIPLSGLYFSGSSRIQTEMQREIEDTVRQTRQELESIDTGSRHLFNTWV
                     DRCRLLFQNTEFVTSITRFDRASLDQAWKKAKDIFGTSGIDTSVICRFGVGIDVLPPP
                     STDDEDRSRHQRARASISGVFPFVSFIAAQLEPTRVHEMFDRFPTLVRSTANIYDQTI
                     DRNAKKDFLLMRAIGDPVQIGDNVVFQYHDAITIDGHLAAWVVLRRRAIDNQQGYLSE
                     ALSRLHLQERNRVFTYGHIRPGYIEFPDTAPVFA"
BASE COUNT          301 a          318 c          389 g          240 t
ORIGIN      
        1 ggcaaagacg ggagcggtgt ccggaaactc gatgtacccc ggacgaatgt gcccgtaggt
       61 aaacactcgg ttgcgttcct gcagatgcag gcgtgaaagc gcttctgaca gatacccctg
      121 ctgattgtcg atcgcacgcc gcctgagaac gacccaggcg gccagatgcc catcgatagt
      181 gattgcatca tgatactgaa agacgacatt gtcgccgatc tgcaccggat caccgatggc
      241 acgcatgagg agaaagtctt tcttggcgtt acgatcgatg gtttgatcat agatgttcgc
      301 cgtggatctg accagggtgg gaaaacggtc gaacatctca tggacgcgag tgggctcaag
      361 ctgcgcggca atgaaggaga cgaaggggaa gactccactg atcgacgctc tcgctcgttg
      421 atgtcgcgaa cgatcttcat cgtcagtgct tggggggggc agaacatcga tgccgacacc
      481 gaaacggcag atcacactgg tgtcgattcc actcgtgccg aaaatatcct tggctttctt
      541 ccatgcctga tcaagactgg cgcgatcgaa tcgggtgatg gaggtcacga actccgtgtt
      601 ctgaaacagc agacgacatc gatcgaccca ggtgttgaac aggtgacgtg atccggtatc
      661 aatgctttcc agttcctgcc gtgtctgacg aaccgtatct tcgatctcgc gttgcatctc
      721 ggtttggatg cgcgaagaac ctgaaaagta gagaccggag agaggaatcg caccgatgag
      781 cgcgaaaaag gcgaacagcc aggtgcgcaa gccaagggcc agctggtgac gggtaagcag
      841 cgtgcgcagg agcagcaacc cccacgttcc tccgaaccag cacaacagga tgaagccgat
      901 attgtcgtga gtcgtaatga tgaggtggcg tcgcgtcggg ctccatagcc agagttcgaa
      961 gggaatgtcg ccctggatga tggtacgata cgcgtgaaac gaggcatgaa aggccagcac
     1021 gctgatcgag gcaatcgact ctcgcggtaa cgtgagaacc ttcttggtga gaggttcgac
     1081 aaactcagga actggtacgg acattgggac catcggctga agtggccgcg ctgagctggc
     1141 gagaggaacc aggacgggga accagcggtt gccgtcgctc acagcagcgc tttccagtgc
     1201 atgacgaagc ggtcgtcccc gggtttcgat cgactcggag aagatgag
//
LOCUS       DAORHD010001572         1247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_158489_length_1247_cov_2.984899, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001572 DAORHD010000000
VERSION     DAORHD010001572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158489_length_1247_cov_2.984899"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..>1247)
                     /locus_tag="PKO06_23575"
     CDS             complement(239..>1247)
                     /locus_tag="PKO06_23575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2181 domain-containing protein"
                     /protein_id="HNV72710.1"
                     /translation="PYRDEPVSPATVPAVGATKPEPPARKSSWFDRVKKPFKSVAKKA
                     TDAVAKTRDLFDTIGDIWDEVTNIPGWVRQGHLDEKKRKELENLARARPSLAWPVNGD
                     IADARNAHRTNTFAEMRDALKGEYDWLECDVRLEGPLRDHVGVPLGDRRPITAHDSFQ
                     TNGLLFEDWVKIAKESGRGIKVDFKDSKALDQVLTLLKNAGVPDHRLILNIGIPDPKP
                     GETDLTKPVSDERLQKIRAAFPQALINLSPGGGTVDGQYSEAQIAQMIRYAKAAGQPV
                     MFPLRAEWVTREVVQKLEPHGKVAIWNSPNTFNPADPAAEIARFRSWGVTGMIDLMTT
                     H"
BASE COUNT          238 a          365 c          355 g          289 t
ORIGIN      
        1 ccgaggctga aacctcgtcg acttcgcatc cctgcgaaga cgacgaggcc gaatcacctc
       61 ctgcgatccg gcagcctgtt ctcggactcc gcccgcttct tgccaaactg ctcacaagtt
      121 tcgctcttgc gaacggaaca tgggtgagtt ttacatgacc ctggtttttc gcagggcgat
      181 tcacctctcc tcaagaagtg gttatcggtc gcatcgcttc acgtgacgtc aacaaagctc
      241 agtgagtggt catgaggtcg atcatgccgg tgacacccca cgaacgaaac ctggcgattt
      301 cagccgcggg atcagcgggg ttgaaggtat tcggagagtt ccagatcgca accttgccgt
      361 gcggctcgag tttttgtact acctcgcggg tcacccactc ggctcgcaga gggaacatca
      421 caggttgccc tgcggctttc gcataccgaa tcatctgcgc gatctgcgct tcagaatact
      481 gaccatcaac agtaccgcca ccggggctca ggttgatgag ggcctgggga aatgccgcgc
      541 ggatcttttg caggcgttca tcggaaaccg gcttggtgag atcggtttcg ccgggtttcg
      601 ggtcgggaat accgatgttg agaatcagcc ggtgatcagg aacaccagca ttttttagca
      661 gagtcaacac ctgatcgagt gctttgctgt ctttgaaatc gactttgatt cctcggcccg
      721 attccttggc aatcttcacc cagtcttcga acagcaaccc attggtctga aacgaatcgt
      781 gcgcggtgat cggccggcga tcgcccagcg gcacgccgac gtggtcacgc agggggccct
      841 cgagacgcac gtcgcattcg agccagtcat actctccttt gagagcatcg cgcatttctg
      901 cgaacgtatt ggtacgatgg gcattgcgag catcggcgat gtcgccgttc accggccagg
      961 ccaggctcgg acgcgcccgt gccaggttct cgagttcctt gcgtttcttc tcgtcgagat
     1021 gcccctgccg cacccaccct ggaatgttcg tcacttcatc ccagatatcg ccgatggtat
     1081 caaagaggtc acgggtcttc gcgacggcat cggttgcttt ctttgcgaca gatttgaagg
     1141 gctttttcac tcggtcgaac caggacgatt tcctggctgg gggttcgggc ttggtcgcgc
     1201 caaccgcagg aacagtcgcg ggagataccg gttcgtctcg atacggc
//
LOCUS       DAORHD010001573         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_159104_length_1244_cov_3.665265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001573 DAORHD010000000
VERSION     DAORHD010001573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159104_length_1244_cov_3.665265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKO06_23580"
     CDS             complement(<1..439)
                     /locus_tag="PKO06_23580"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV72711.1"
                     /translation="MSLFPRLLLMGLFMVVLCVGFLVGAYYEIERAFAPTPVLAQAVT
                     VTIPAGAGSHEIGAALKQAGIIRSSFWFRVMVSWYAIDQRLKPGSYPFRGGEDLNTVI
                     HALVQGREEKVRVTIPEGLKLVEIAQVVERAGIASAATFLQAIQ"
     gene            complement(459..>1244)
                     /locus_tag="PKO06_23585"
     CDS             complement(459..>1244)
                     /locus_tag="PKO06_23585"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV72712.1"
                     /translation="FISPSDMGIAGNGDIIVADSGNNRIQVLAGNDLSCKFSFGEFGS
                     GGNKFLGISGLAVNRLSGEIAVCDLKGHKISKFDSTGKQILYFTDKVRCPLDIEFDKL
                     GNMYVIMTKQPGIYKYNQRGVFQEIVGGKGKAAFVFATSITFANDHLYIADYGAKRIV
                     KMTLSGEVVREVTQKGEYEELQGPRGVGVDDKENYYILDLGEVPISMLTKEGALISRI
                     GVFGKEPGQFLYPRTILARNEGDVLILDYTRNVILNFKKMATK"
BASE COUNT          300 a          355 c          321 g          268 t
ORIGIN      
        1 tctgaatcgc ctgcagaaag gtggccgccg aggcaattcc ggctcgttcc actacctgag
       61 cgatctcgac cagtttgagc ccctcgggaa tcgtgacgcg gactttttct tcgcgaccct
      121 gaaccagggc atgaatcacg gtattgagat cttctccgcc acgaaacggg taggaaccgg
      181 gcttcaaacg ctgatcgatc gcataccacg agaccatgac gcgaaaccag aaactgctgc
      241 gaatgatacc cgcctgcttc aacgcggcac caatctcgtg gctgccggca ccggcgggaa
      301 tggtcactgt caccgcctgt gccagcacgg gagttggagc aaaagccctc tcgatctcat
      361 agtatgcgcc gaccagaaag ccgacgcaca ggacgaccat gaacagcccc atcaggagca
      421 ggcgagggaa aagggacatc aaaacgtgcg ctgaaccgct acttcgtcgc catcttcttg
      481 aaattgagaa tgacgttgcg cgtgtaatcc agaatcagga catcgccctc gttgcgagca
      541 agaatcgttc ggggatagag aaactgcccc ggctctttgc cgaagacgcc aatgcgggag
      601 atcagagccc cttccttggt cagcatcgag atgggaacct cacccaggtc aaggatgtag
      661 taattttctt tatcgtcgac acccacaccg cgagggccct gcagttcctc gtattcacct
      721 ttctgcgtga cttcacgaac aacctcaccc gacaaggtca ttttcacgat ccgcttggct
      781 ccgtaatctg cgatatacag gtggtcattg gcgaacgtaa tgctggtcgc gaacacgaat
      841 gccgccttgc ctttgccacc gacgatctcc tggaacacac cgcgctggtt gtatttgtag
      901 atgccaggct gcttggtcat gatgacgtac atgttcccga gtttgtcgaa ttcgatatcg
      961 agagggcacc gcaccttgtc ggtgaaatac agaatctgtt tccctgtgga gtcgaacttg
     1021 ctgattttat gccccttcag atcgcacacg gcaatttcgc cagacagacg attcacggct
     1081 aatccggaga tgccaaggaa tttgttaccc cccgaaccga attctccgaa tgagaatttg
     1141 caggaaaggt cattgcccgc aagcacctga atgcgattgt ttccggagtc agcaacgatg
     1201 atgtcgccat tgccagcaat acccatgtca gagggtgaga taaa
//
LOCUS       DAORHD010001574         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_159325_length_1243_cov_3.787879, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001574 DAORHD010000000
VERSION     DAORHD010001574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159325_length_1243_cov_3.787879"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKO06_23590"
     CDS             <1..453
                     /locus_tag="PKO06_23590"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HNV72713.1"
                     /translation="APDKPQLVGAMAQGAPEHHRMCRWGPDSHVPPRAPHIMETNAVG
                     LLKHGAALVERLGKAFFQEVHWARHEIDRLISHQVATANREAILQALGIPHDRDFNTF
                     PYLGNMGTVSLPVTAAIAAERGILERGQKVAFIGIGSGLNSMMLGWKW"
     gene            460..>1243
                     /locus_tag="PKO06_23595"
     CDS             460..>1243
                     /locus_tag="PKO06_23595"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV72714.1"
                     /translation="MSFDPSSVPDQYPFKGRYFERHGLRLHYLDEGNVAGQPEKPPLI
                     CVHGNPSWSFLYRGIAKAFSERLRVIVPDHIGCGLSDKPGLDRYDYRLKSRIDDLEAL
                     IDSLKLTQPLTLMVHDWGGMIGFGYAVRHPERVARLVVTNTAAFGLPQGHPFPWPLYA
                     FRNWKLGAWINEQFNAFSEIASYTCTVKPLAPEIRRAFVAPYDTPANRVAVTRFVQDI
                     PLVPGDPSWEALQQTESGLPKLARKPMLICWGRHDFVFDRGFF"
BASE COUNT          226 a          381 c          369 g          267 t
ORIGIN      
        1 gcccccgaca aaccccagct ggtgggtgcc atggcgcagg gagcgcccga acatcaccgc
       61 atgtgccgct ggggtcctga ctcccacgtt cctccccgtg ctccccatat catggaaacc
      121 aacgcggtcg gactgctcaa acacggagcg gccctcgtcg agcggcttgg caaggccttc
      181 tttcaggaag tgcattgggc caggcacgag atcgatcgcc tcatttcgca tcaggtggcg
      241 acagccaacc gcgaagcgat tctgcaggct ctgggcattc cgcatgaccg cgacttcaat
      301 accttcccct accttggcaa catgggtacc gtctcgctcc ctgtgacggc cgcgatagcg
      361 gctgaacgcg gtatcctcga gcggggacag aaagtagcct tcatcggcat cggcagtggt
      421 ctcaacagca tgatgctggg ctggaagtgg tagggtgagg tgagtttcga tccctcgtcc
      481 gtgcccgacc agtacccctt caagggtcgg tatttcgaac gtcacggcct gcggctgcat
      541 tatctcgatg aagggaacgt ggccggccaa cccgagaaac ctccgctgat ctgcgttcac
      601 ggcaatccgt cgtggtcgtt cctctaccgg ggaatcgcca aagcgttttc tgagcgcctg
      661 cgggtgatcg tgcccgatca tatcgggtgt ggtctttctg acaaacccgg tctcgatcgg
      721 tatgattatc gtctgaaatc gcgcatcgac gatctcgaag cgctgatcga ttcgttgaag
      781 ctgacgcagc ccctgaccct gatggtgcat gactggggcg gcatgatcgg gttcggctac
      841 gcggttcggc accctgagcg tgtggcgcgg ttggtggtga ccaacacggc tgcgttcggt
      901 ctgccgcagg ggcatccgtt tccgtggccg ctctatgcct tccgcaactg gaaactcgga
      961 gcctggatca acgaacagtt caatgctttt tccgaaatcg cctcgtacac ctgcacggtg
     1021 aaaccgctgg ctcctgagat tcgacgggca ttcgtggcac cctacgacac ccccgcgaat
     1081 cgcgtggccg ttacgcgatt tgtgcaggat attccgcttg taccgggcga tccatcgtgg
     1141 gaagctcttc agcagaccga atcgggcttg ccgaaactcg cccggaaacc gatgctgatc
     1201 tgctggggtc ggcacgattt cgtcttcgat cgcgggttct tca
//
LOCUS       DAORHD010001575         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_159528_length_1242_cov_4.112890, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001575 DAORHD010000000
VERSION     DAORHD010001575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159528_length_1242_cov_4.112890"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1054)
                     /locus_tag="PKO06_23600"
     CDS             complement(83..1054)
                     /locus_tag="PKO06_23600"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNV72715.1"
                     /translation="MYLANVSKKLLLLVQIALVFLATVAFASEPLLPGDQIHIWVKGE
                     PDLTVDRTVGQEGSINFPLIGSVKVAELIPAEAAKLIARKLDDGYIREPLVQINRIGS
                     RALLSDVRVKPAAARPRVSNPQPQLEETPVVSAEPLATSEELTTTAPVEVPRVQRPVR
                     TVPRVPAASYVEIIDGKTGAGVRSAVMLFNGKIFQTNSAGQMKLDALQGHLILMADGY
                     SVVQGQIQHYLKPGVMPKIVMDPIPLADEIEIRVVDVGTSLPLSDVQVKLNGLRAKTN
                     RSGTFRIKELKAEYSEVELSKKGYRTIRQIIDSKDSRPRTIALVPAN"
     gene            complement(1146..>1242)
                     /locus_tag="PKO06_23605"
     CDS             complement(1146..>1242)
                     /locus_tag="PKO06_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005406579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72716.1"
                     /translation="KAEGIFLARRTVAKYRSELNIQSSSTRKRSV"
BASE COUNT          271 a          306 c          395 g          270 t
ORIGIN      
        1 aacgactgat cgcgtgagac ttcggaaggg aacgcaaaga aaacgatgga aagaaggggg
       61 gctggttgct gagggtgagc cgtcagttcg cgggaaccag agcaatggtg cgcgggcggc
      121 tgtccttgga gtcgatgatc tgacgaatgg tgcgataccc ttttttcgac agttcgactt
      181 cgctgtattc tgctttcagt tctttgatgc ggaaagtacc ggagcgattg gtcttcgccc
      241 ggagaccgtt cagtttcacc tgaacatcag agaggggcag gctggtaccg acgtcgacga
      301 ctctgatttc gatctcatcg gcgagaggaa tgggatccat cacgatcttg ggcatgacac
      361 cgggtttgag atagtgctgg atctgtccct ggacaacgct gtatccgtcc gccatgagaa
      421 tcagatgacc ctgcagagca tcgagcttca tctgaccggc tgagttggtc tgaaagatct
      481 tgccgttaaa gagcatgact gcagacctga caccggcccc ggtcttgcca tcgataatct
      541 caacgtagct ggcagcgggc acccggggga ccgtccgcac ggggcgctgg actcggggga
      601 cttcgacagg ggctgtcgtg gtcagttctt cagaggttgc cagcggttcg gcagatacca
      661 ctggggtttc ttccagctga ggttgtgggt tcgagacgcg gggcctggct gcggcgggct
      721 tcacgcgcac atcggagagc agggcacgac tgccgatgcg attgatctgc acgagcggtt
      781 cacggatgta gccatcatcg agcttgcgcg caatcagctt ggcggcttct gcgggaatga
      841 gttcggcaac cttgaccgat ccgatgagcg ggaagttgat gctgccttcc tgaccgacgg
      901 tgcgatcgac ggtcaggtcg ggttcgcctt tgacccagat atggatctga tcgcctggaa
      961 gcaggggttc ggaggcgaaa gccactgtcg ccagaaacac gagggcgatc tggacgagca
     1021 gtagcaattt ctttgacacg ttagcgagat acatattttc tcaaaagata tcgacggatt
     1081 ctcagcaaac gttaagagaa aaaaagggaa aaacggaaaa agctgtgccc tcggggtgtc
     1141 gccgcttaaa cagaacgttt gcgggtcgaa gaactctgaa tgttcaattc gctgcggtac
     1201 ttcgctaccg tgcgtcgggc cagaaagatg ccctcggctt tg
//
LOCUS       DAORHD010001576         1241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_159924_length_1241_cov_1.911467, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001576 DAORHD010000000
VERSION     DAORHD010001576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_159924_length_1241_cov_1.911467"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1241
                     /gene="dnaB"
                     /locus_tag="PKO06_23610"
     CDS             <1..>1241
                     /gene="dnaB"
                     /locus_tag="PKO06_23610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV72717.1"
                     /translation="NAVAEVIDILRPEAFYKEAHSKIFAAIQQLFHTTQPVDILTVTQ
                     QLRKNGDLELIGGPYFITQLTNRVASAANIEFHARIVLQKYIQRELIRISGETIRDAF
                     EDTSDVLELLDRAERNLFGIAESNIRRKYETMSGLVAKALKEVETAASQKSGVTGVPS
                     GFTDLDRVTAGWQKSDLIIIAARPGMGKTAFVLSLARNAAVQFNKPIAFFSLEMSSIQ
                     LVNRLVSSETEIDTEKLKKGRLESHEWQQLNMRVSPLTNAPIYIDDTPALSVFELRAK
                     CRRLAAEKKIEMVIIDYLQLMVGGQDNNKNGNREQEISHISRSLKGIAKELNIPIIAL
                     SQLSRNVEKSQTKKPQLSDLRESGAIEQDADMVMFIYRPEYYGLTETEDNLPATNLAE
                     IIVAKNRNGALKSVNLKFLGH"
BASE COUNT          365 a          315 c          288 g          273 t
ORIGIN      
        1 aaacgcagta gcggaggtaa ttgatattct tcgtccggaa gccttttaca aagaagcaca
       61 tagtaagatt tttgcggcta ttcagcagct ctttcacact acacaacctg ttgatattct
      121 cacggtaacg caacagttac ggaagaatgg tgatctggaa cttattggag gtccgtattt
      181 catcacacag ctcacgaacc gtgtagcttc tgcagccaac attgaatttc acgcaaggat
      241 tgtcttgcag aaatatatcc agcgagaact gatccggatc tcaggggaaa ctattcgtga
      301 tgcttttgaa gacacctctg acgtactcga gttgttagat cgtgcagagc gaaatctctt
      361 tggaatcgca gaaagtaata tccgtcggaa atacgaaacg atgtcggggc ttgtagcaaa
      421 agcactgaag gaagtagaga ccgcagcttc acagaagagt ggagtaacag gtgtacccag
      481 tggatttacc gacctcgacc gcgtcacagc cggctggcag aaaagtgatc tcatcatcat
      541 tgcagctcga ccgggtatgg gtaaaaccgc cttcgtattg agtctggccc gcaatgctgc
      601 tgtacagttc aacaaaccca tcgcgttttt cagtctggag atgtcatcga tacagctcgt
      661 aaatcgtctc gtctccagtg aaacagaaat cgacaccgag aaactcaaaa aaggacgcct
      721 cgaatcgcat gaatggcaac agctcaacat gcgcgtttcg ccgctcacaa atgcacccat
      781 ctacatcgat gatacacctg cactgagcgt attcgaattg cgtgctaaat gccgacgact
      841 cgcagccgag aagaaaatcg aaatggtcat catcgactat ctccagctga tggtcggtgg
      901 acaggataac aacaagaacg gcaaccgtga acaggagatc tcccatattt cccgatcgct
      961 caaagggata gccaaggagt tgaatattcc catcatcgcc ctctctcagt taagtcgaaa
     1021 cgttgaaaaa agccagacca agaagccgca gctcagtgac ctccgtgaat cgggagcgat
     1081 tgagcaggat gccgacatgg taatgttcat ctaccgccct gaatactacg gactcaccga
     1141 aacggaagac aatctccccg ccaccaacct cgcagagatc attgtcgcca aaaacaggaa
     1201 tggagccctc aagagtgtca acctcaagtt ccttggccat c
//
LOCUS       DAORHD010001577         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_160025_length_1240_cov_3.318143, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001577 DAORHD010000000
VERSION     DAORHD010001577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160025_length_1240_cov_3.318143"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PKO06_23615"
     CDS             <1..705
                     /locus_tag="PKO06_23615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72718.1"
                     /translation="RAWTFLKKQETEQSLREFRQLQTTFPGDPAVLGGLREAERLQLN
                     GALQEAPTAEHLPGLREQGRFFEALQLSDALLAQNSDQRLVLSERAKALMALARWKEA
                     EECFAMLLQKEAAPEYRRERMMALWSLGRLQEAAVEAREIVQTEPNHVLAIKIVADDL
                     YTVGRFEQSLTWYQKLPEDLWAWLGQGWCHLVMGKKELARPLFQKCLMAYPGHTSAIE
                     GLRRSEELATGTNTGH"
     gene            728..>1240
                     /locus_tag="PKO06_23620"
     CDS             728..>1240
                     /locus_tag="PKO06_23620"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNV72719.1"
                     /translation="MKNTFIFLVVCLLCSVAAMSAPPAAVPALNAEIRQLPSLTYLCL
                     EGKGPYTRIPEVAKEFMGYFASSGAKAIDKELLIYWNSPLYVKPAELRWDIGFPVNGG
                     QGDTQRLKAKKFPYRKIAVATHVGPYYTTYLTINALYTWITKNGYKTVGGPCVERYID
                     DPTSSIPDAQR"
BASE COUNT          276 a          320 c          373 g          271 t
ORIGIN      
        1 cgagcctgga cgtttctgaa aaagcaggaa acggaacaat cgctgcgcga gtttcgtcag
       61 ctccaaacaa cttttcccgg tgaccctgcg gtgcttggtg ggttgcggga agcggaacgc
      121 ctacagttga acggcgcatt gcaggaagct cccactgctg agcacctgcc tggacttcgt
      181 gaacaaggtc ggttcttcga agccttgcag ctttctgatg ctctgctggc tcagaattca
      241 gaccagcgtc tcgtactgtc ggaacgagcg aaagcattga tggctctggc gcgctggaag
      301 gaagccgagg agtgttttgc catgctgctg cagaaagagg cagcgcccga gtatcgccgc
      361 gaacggatga tggcattgtg gtctcttgga aggttgcagg aagcggctgt tgaagcgcga
      421 gagattgtac aaacagagcc gaatcatgtt ctggcgataa aaatcgtcgc tgacgatctc
      481 tacacggttg gccggttcga gcagtcgctg acctggtatc agaagcttcc cgaagacctg
      541 tgggcgtggc tcggtcaggg gtggtgccat ctggtcatgg gaaagaagga actcgccaga
      601 ccgttgttcc agaagtgtct gatggcgtat ccgggccata catcggcgat cgagggctta
      661 cggaggtctg aagagcttgc gaccggtaca aacaccggtc attgagattt tcgtgaggag
      721 aaacagcatg aaaaacacgt tcattttcct ggtggtctgt ctgctctgtt ctgttgcggc
      781 gatgtctgca cccccagctg cggttccggc gctcaacgcc gaaatcaggc aactgccttc
      841 tttgacgtat ctgtgcctcg aggggaaggg gccgtatacc cgcattccgg aagtggcaaa
      901 agagttcatg ggctacttcg cctcctccgg tgccaaggcg atcgacaagg aactgctcat
      961 ctattggaac agcccgttgt atgtgaagcc cgccgagttg cgctgggata tcgggtttcc
     1021 ggtcaatggc ggccagggag acacacagcg tctgaaagcg aagaagtttc cgtatcggaa
     1081 aatcgcggtt gcgacacatg ttggcccgta ttacaccacc tacctgacga tcaacgccct
     1141 gtatacctgg atcaccaaaa acggctacaa aacggttggc ggtccctgcg tcgagcgcta
     1201 tatcgatgat ccgaccagtt cgattccgga cgctcagaga
//
LOCUS       DAORHD010001578         1238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_160499_length_1238_cov_2.989856, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001578 DAORHD010000000
VERSION     DAORHD010001578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160499_length_1238_cov_2.989856"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="PKO06_23625"
     CDS             <1..1122
                     /locus_tag="PKO06_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72720.1"
                     /translation="HPCELASSHNPPEAHATEPQEEKRLARELARRLDLQVGCAADRQ
                     TSWLAALATTPGTCREVAPGTEDAFRASLPIVTLGDGGISADTLERLQWLGITTVGCL
                     TRFRRHQLMARFPEGERLFEMARNGDRRPVSLYTPPPEVRGRYLDAAATLTERGEIQG
                     VLRHLLDEAVVRLDARSVGQITLTLAGGGRRLSRRRLLRTATASPRSLRTAIDRLLDD
                     LLSELSASDVLSVGSSGFETSETSISSLVSADRPFAARTPRVRAAGPRGPGASGPWRG
                     QAGQSPRPFLSSPSVSAFEFDEVELVLGRLSVPSSVQGRLWLGPDRERVRFEQNLRLL
                     EKRFPGRLRRIVLHDPDSYLPEDAFTLVSAVPHKEPVCD"
BASE COUNT          171 a          413 c          384 g          270 t
ORIGIN      
        1 catccgtgtg aactggcttc ttcgcacaac ccaccagaag cgcatgctac ggagccccag
       61 gaagaaaaac gtcttgcgcg ggagctggct cgtcggctcg atctgcaggt cgggtgcgcc
      121 gccgatcgtc agacctcgtg gctggcggct ctggctacca cgcccggcac ctgccgcgaa
      181 gtcgctcctg gtacggaaga cgccttccgc gcctctctgc cgatcgtgac cctgggcgac
      241 ggcggcatca gcgccgatac tctcgaacgt ctgcagtggc tcggcatcac cacggtcggt
      301 tgcctgaccc gctttcgccg tcatcagctt atggcccgtt tccccgaagg agagcgactg
      361 ttcgagatgg ctcgcaacgg tgaccgtcgc ccggtttctt tgtatacccc gccgcccgaa
      421 gtgcgggggc gatacctcga tgcggcggcg acgcttactg agcgcggcga gattcagggc
      481 gttctgcgac acctgctcga cgaggccgtg gttcggctcg atgcccgcag tgtcggccag
      541 atcacgctga ccctggcggg cggcggccga cgcctcagcc gccgacgcct gctccgcacc
      601 gcgaccgcgt cgccgcgttc tctgcgcacc gccatcgacc gcctgctcga tgatcttctg
      661 tccgagttat ctgcttctga tgttctctcg gttggctcgt ctggtttcga gacatccgaa
      721 acatctattt cttctcttgt gtctgctgat agaccctttg ccgctcgaac gccccgcgtt
      781 cgagcggcag ggcctcgggg tccaggggcc tccggtccct ggcgggggca agcggggcag
      841 agcccccgtc ctttcttgtc ttctccttct gtttctgcgt tcgagttcga tgaagtcgag
      901 ctggttctgg gtcgtctgtc cgtgccgtcg tccgtccagg gtcgcctctg gctcggccct
      961 gatcgtgagc gggttcggtt tgaacagaat ctgcggcttc tcgaaaagcg ctttccgggg
     1021 cggttgcggc gtatcgttct gcatgatccc gacagttatc ttccggaaga cgccttcacg
     1081 ctggtttcgg ccgttcccca caaggagccc gtatgcgact gatgctcgaa ccgattgtcg
     1141 tgaccctcga acgcgctctg ccgcgcacct tcagctggcg gcggcagaga cgcctgatca
     1201 gtcgcgtgct cgaacagtgg cagtggcgca gtcgctgg
//
LOCUS       DAORHD010001579         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_160929_length_1236_cov_3.011008, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001579 DAORHD010000000
VERSION     DAORHD010001579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160929_length_1236_cov_3.011008"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKO06_23630"
     CDS             <1..238
                     /locus_tag="PKO06_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72721.1"
                     /translation="VISEKKSETIMEDIDRLIFYRFKDSATSGGSTSSIPSAKCVFFD
                     LFMTRLDKEGTVDQKYTGKLTTSVQVRGSMPEGF"
     gene            385..>1236
                     /locus_tag="PKO06_23635"
     CDS             385..>1236
                     /locus_tag="PKO06_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72722.1"
                     /translation="MILIAALFSFSSARIQNTLMESQMLKALAVAEAGLQFAICELYQ
                     DPGFATHDLDANQEWTNTAKAHSNYVSDLGTPLNLKITSGTNGTYEGDFGEGHFKVRV
                     GPINPTSFGDNPNTKNINEATCFLRIDSMGKVKDYIKHVSAVVMRRYPGREFLMYSGD
                     VLSMVYGLKGSGYASQFNVFSTGHLYGHRGIEIGPVMLTQQGSQGVGTRQKLADLELL
                     SSGLGGIFFYDPQGTPIQFRGDSAETTISQNVDPAVFQNGGNFAPGANRELGSFPSSL
                     VDEKGNLV"
BASE COUNT          271 a          354 c          332 g          279 t
ORIGIN      
        1 ggttatttcc gagaagaaat ccgagacgat tatggaagat attgatcgcc tgattttcta
       61 ccgtttcaag gactcggcca cgagcggcgg cagtacttct tcgatcccgt cagcaaaatg
      121 cgtctttttc gatctgttca tgacccggct cgacaaggaa ggtacagtcg atcagaagta
      181 caccggcaaa ttgaccacct cggttcaggt gcgcggctcc atgcccgagg gtttttagcc
      241 atgaaaggga atcctatgct gaaacaacga acacccggca tcgccagttc cggcttccgt
      301 ccaggttggt tcgcattcat tttcgcatca cgtcaacgtt ccgggtcggc cgttcttatg
      361 gcgctcgggt tcggggtcgt attgatgatc ctcatcgccg cgctgttcag cttttcgtct
      421 gcccgcattc agaacacgct catggaaagc cagatgctga aagcattggc ggtagccgag
      481 gccggcctgc agtttgccat ctgtgagttg tatcaggacc ccggattcgc cacccatgat
      541 ctcgacgcga atcaggagtg gacgaatacc gccaaagcgc actcgaacta tgtttccgat
      601 ctggggacgc cactcaacct gaagatcacc agcggcacga acggcaccta cgagggcgac
      661 ttcggcgagg gacacttcaa ggttcgcgtt gggccgatta atccgacgag cttcggcgat
      721 aatccgaaca ccaagaacat caatgaagcc acctgttttt tgcgcatcga ctccatgggg
      781 aaagtcaaag attacatcaa gcacgtttcg gccgtcgtca tgcgccgcta ccccggtcga
      841 gaatttctca tgtactcagg cgatgttttg tcgatggtct acggcttgaa gggcagcggt
      901 tacgcaagcc agttcaatgt cttttccacc ggccacttgt acggtcaccg cggcatcgag
      961 atcggtccgg tcatgctgac acaacaggga tcacagggcg tggggacgcg ccagaaactc
     1021 gctgatctcg agctgttatc gagcggactc ggtggcatct tcttctatga cccgcaggga
     1081 accccgattc agtttcgcgg cgactcggcc gaaaccacta tttcgcagaa cgttgatcca
     1141 gccgtcttcc agaacggcgg gaatttcgct cccggtgcca accgcgagct tggttcattc
     1201 cccagcagcc tggtcgacga aaaaggcaac cttgtc
//
LOCUS       DAORHD010001580         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_161188_length_1235_cov_2.724576, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001580 DAORHD010000000
VERSION     DAORHD010001580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161188_length_1235_cov_2.724576"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..612
                     /locus_tag="PKO06_23640"
     CDS             85..612
                     /locus_tag="PKO06_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV72723.1"
                     /translation="MEALTAISGRRSIRRFAPGPVGADQVKALLTAAMSAPSAGNQQP
                     WHFVVTDDASILARVPAHHPYASMVPSASLAVLVCGDLQLEKFPGFWVQDCAAATENL
                     LVAAHALGLGAVWLGVHPMPDRENGMRRLFELPDEVIPFALIPIGRPAEEKPATDRYQ
                     PLRVYKDRWGTSFGL"
     gene            609..>1235
                     /locus_tag="PKO06_23645"
     CDS             609..>1235
                     /locus_tag="PKO06_23645"
                     /inference="COORDINATES: protein motif:HMM:NF014681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HNV72724.1"
                     /translation="MTTRLTSRRRARNAASSSEPLADLQSHLDHRRLPIARVGIKRLF
                     YPFRVLDRRRTFQDVTGEFEVYVDLTHTERATHMSRFVEIINHHRGEVTSNTIESILK
                     DVKKRLQARRAELAVSFPYFVEKHAPVSGEASLMRYDCRFWAALDRTLDFVLTVTVPV
                     TTLCPCSKAISKASAHNQRSLVTVAIRFRRMVWIEEVIACVEASASAEV"
BASE COUNT          216 a          381 c          373 g          265 t
ORIGIN      
        1 gaaagctttt ccatctgttc atgcttgtgt taccctcgcg cagggttctc ccctgtgtac
       61 catattccgc ccccggagga aaacatggaa gctctcaccg cgatcagcgg ccgccgcagt
      121 attcggcgtt tcgctcccgg cccggtcggc gccgaccagg tcaaagcgct tttgacggcg
      181 gccatgagtg ccccgtcggc tggaaaccag caaccctggc acttcgtggt gaccgacgat
      241 gcctcgattc tggcgcgcgt gcccgcgcac catccgtatg cgtccatggt tccatcagct
      301 tccttggcgg ttctggtctg tggggacctg cagcttgaga agtttccggg tttctgggta
      361 caggattgcg cggccgccac cgaaaacctg ctggttgcgg cacatgcgct gggcctcggg
      421 gcggtctggc tgggggtgca tcccatgcct gatcgcgaga atgggatgcg gcgattgttc
      481 gagcttcccg atgaagtcat tccctttgcg ctgataccga tcggccgacc ggccgaagag
      541 aaacctgcga cagaccgcta ccagccgctt cgtgtctaca aagaccgctg gggaaccagc
      601 ttcggcttat gacaacgcgt ctcacctcgc gtcgccgcgc caggaacgcc gcgtcgtcgt
      661 cagagccgct ggccgatctg cagagccacc tggaccatcg ccgcttgcct atcgcgcggg
      721 ttggtatcaa acgtctgttt tacccttttc gcgtgcttga ccgccggcgc acgtttcagg
      781 atgtcaccgg agaattcgag gtctacgtcg atctgacgca taccgaacgg gcgacgcata
      841 tgagtcgttt cgtcgagatc atcaaccatc atcggggaga ggtcacctcg aacacgatcg
      901 agagcattct gaaggatgtg aaaaaacggt tacaggcgcg tcgtgccgag ttggccgtca
      961 gttttcccta cttcgtcgag aaacacgcgc cggtttcggg cgaggccagt ctcatgcgct
     1021 atgactgccg tttctgggcc gcgctcgatc gtacgctcga tttcgtgttg accgtgacgg
     1081 tgcccgtgac cacgctttgc ccctgcagca aggcgatcag caaagcgtcg gcacacaacc
     1141 agcgcagtct ggtgacggtg gccattcggt ttcggcgcat ggtctggatc gaagaagtca
     1201 tcgcctgcgt cgaggcgtcg gccagtgccg aagtc
//
LOCUS       DAORHD010001581         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162061_length_1231_cov_3.493197, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001581 DAORHD010000000
VERSION     DAORHD010001581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162061_length_1231_cov_3.493197"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PKO06_23650"
     CDS             complement(<1..1082)
                     /locus_tag="PKO06_23650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HNV72725.1"
                     /translation="MIIYTSGTTGKPKGVMLTHKAVCAAVRIGIQVGNLQAGDTMLVL
                     LPLTHVFGLVDSGLCPLAVGARVVMSNSFSPPDIMAAVQRYRVNYINAVPRLAEVLLA
                     VMPPSPAIQALGIKMFIGGAACRPEVIKGLQDRGIKAVQGFGMTETAGGIIASTLSSP
                     ADSVGKPGAEVQIRIDAPAGQVGEILIKSPTNTSGVFGKPEMYDELYHDGFLRTGDLG
                     EFDKDGNLKIRGRLKDLIIPPGGMNVYPDELELRLGRLPFAEEFAVIGFHEGGHEYPA
                     LVVRPNLKEFAGQAMGSVLEIVQQKVQTLIENWPEWEKFRRVICVEAPLPRSPSMKIQ
                     RNVLMQAVGAATPGSDRLPDKAVANGA"
BASE COUNT          266 a          378 c          336 g          251 t
ORIGIN      
        1 gcaccattcg cgacagcttt gtccggcagt cgatcagagc cgggagtcgc agctcccaca
       61 gcctgcatca acacattccg ttgaatcttc atgctgggcg aacggggcaa cggtgcttca
      121 acgcagatga cccggcgaaa cttttcccat tcgggccaat tctcgatgag cgtctggacc
      181 ttttgctgca cgatctcgag aacacttccc atcgcctgcc ccgcgaactc tttcagattc
      241 gggcgcacga ccagtgccgg gtattcgtga ccaccctcgt gaaaaccgat caccgcaaat
      301 tcttcggcga acggcaaacg ccccagacgc agttcgagct cgtctgggta aacattcatg
      361 ccgcccggcg gaatgatcaa gtctttcaac cgaccccgaa ttttcagatt tccatcttta
      421 tcaaactcgc caagatcgcc agttctcaaa aatccatcgt ggtacaattc gtcatacatc
      481 tctggcttac cgaagacacc cgacgtgttt gtcggcgatt tgatcagaat ctcgccgacc
      541 tgcccggccg gcgcatcaat gcgaatctgc acctccgctc ccggtttgcc gaccgaatcg
      601 gccggagatg agagtgttga agcaatgatt ccaccggcgg tttcggtcat gccaaagcct
      661 tgaacggcct tgatgccgcg atcctgcagc cccttgatca cttcagggcg acaggcggct
      721 ccaccgatga acatcttgat gccgagcgcc tgtatggccg ggctcggcgg catcaccgcc
      781 agcagcacct cggcgagtcg aggcaccgca ttgatatagt tcacacgata acgttgcacg
      841 gccgccatga tgtcaggagg gctgaacgaa ttggacatga ccacgcgagc gccgaccgcc
      901 agcggacaga gccccgaatc gaccaagccg aagacatggg tcagcggcag aagcaccagc
      961 atggtgtctc cggcctgcag attgcccacc tgaatgccga tgcgcaccgc agcacacacc
     1021 gccttgtggg tcagcatcac tcccttgggt ttgcccgtcg tgcccgaggt gtagatgatc
     1081 acggccaggt catccggttc cggggggggc ggaggggtgt ggatggactg aatatcttct
     1141 ttgacgcaga cgagatcgaa gtgaaagttg accaggatcg tgttgctctt gatctgggga
     1201 aacagcgcaa cgaggcgttc ctgatacttc t
//
LOCUS       DAORHD010001582         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162075_length_1231_cov_3.299320, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001582 DAORHD010000000
VERSION     DAORHD010001582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162075_length_1231_cov_3.299320"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1231
                     /locus_tag="PKO06_23655"
     CDS             <1..>1231
                     /locus_tag="PKO06_23655"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV72726.1"
                     /translation="ANEAGREDLQIVGGGGGVILPQEGEELQKYGVTRIYSPEDGRKL
                     GLLGMISDILRRTDHALAEWPKRLGEGKISWNEPLRLARALTLLENNVDALPAKIKRE
                     IEKGRRDGNLDKNGFDHKAEPPLVLGVTGPGGAGKSSLTDELIRRFLLDFPDRRLCIF
                     SIDPSKRKTGGALLGDRIRMNAINSPNVFMRSFATRGSRCELNVGINDAISLARQAGF
                     DLIIVETSGIGQGDTGILNVSDAALYVMTGEFGAPMQLEKIDMLDFADLVAINKFDRR
                     GSEDAYRDVVRQMRRSRFHGKQVPENECPVFGTMASKYHDDGMNGLYRELLTILNRRC
                     GQELWKPHLELKTARRSSAVAPVIPQNRTQYLSEIADTVREYHQRTDEQMKQANLCQS
                     FDAALTQNLSESAREEIR"
BASE COUNT          261 a          400 c          361 g          209 t
ORIGIN      
        1 tggccaatga ggccggtcgc gaagatctgc agattgtggg cggcggcggc ggcgtcattc
       61 tgccgcagga aggcgaagaa ctgcagaaat acggcgtcac ccgcatctac tccccggaag
      121 acggccgcaa actcgggctg ctcggcatga tcagcgatat tctgcgccgc accgatcatg
      181 cgctggccga gtggccaaag cgcctcggcg aaggcaagat ttcctggaac gaacccctgc
      241 gcctcgctcg cgcgcttacc ctgctcgaaa acaacgtcga cgcccttccc gcgaagatca
      301 agcgcgagat cgaaaaaggt cgccgcgacg gcaacctcga taagaacggc ttcgaccata
      361 aagcggagcc acctctcgtg ctcggcgtga ccggcccggg cggagccgga aagagcagtc
      421 tcaccgacga attgatccgc cggttcctgc tcgacttccc cgatcgccgc ctgtgcatct
      481 tcagtattga cccgtcgaaa cgcaagaccg gcggggcatt gcttggtgac cgcattcgca
      541 tgaacgccat caactcgccg aacgtcttca tgcgcagttt cgccacccgc ggcagtcgct
      601 gcgaactcaa cgtcggcatc aacgacgcga tcagcctggc ccgccaggcc ggcttcgacc
      661 tgatcatcgt cgaaaccagc ggtatcggcc agggtgacac gggcattctc aacgtcagcg
      721 acgccgcgct ctacgtcatg accggcgagt tcggcgcgcc gatgcagctc gagaagatcg
      781 acatgctcga cttcgccgat ctggtcgcca tcaacaagtt cgaccgacgc ggctctgaag
      841 acgcctatcg cgacgtcgtg cgacagatgc ggcgcagccg ttttcacggc aaacaggtgc
      901 ccgagaacga gtgcccggtg ttcggcacca tggcctcgaa gtatcacgat gacggcatga
      961 acggcttgta tcgcgaactg ctgaccatcc tgaatcgccg ttgcggtcag gagctctgga
     1021 agccgcatct cgaattgaaa accgcccggc gcagttcggc ggtcgccccg gtcattccgc
     1081 agaatcgcac ccagtatctc agcgagattg ccgataccgt gcgcgagtat catcagcgca
     1141 ccgatgagca gatgaagcag gcgaacctct gccagtcttt cgatgcggct ctcacgcaga
     1201 atctgagcga atcagcacgc gaggagattc g
//
LOCUS       DAORHD010001583         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162109_length_1231_cov_2.788265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001583 DAORHD010000000
VERSION     DAORHD010001583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162109_length_1231_cov_2.788265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKO06_23660"
     CDS             complement(<1..656)
                     /locus_tag="PKO06_23660"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72727.1"
                     /translation="MNSETNTALDNEQVIRDERERLTSIRSQLLETHPFWGYLLLQMR
                     LIPDVSLPHLAATDCYRTIWYNPLQTRQLNIRQLGFVLAHEIGHSVFATNERQGVRNP
                     FLWNCATDYAINRIVAEICHPGGWQKRPLYEVPNTKLENGEEIKILLDKQYDGMIAEM
                     IYERLAEDELQKPSIIKLTLRMPKQPGQHDDEDAGSGGDNSDNDPGEELVELPNTLDH
                     "
     gene            complement(675..>1231)
                     /locus_tag="PKO06_23665"
     CDS             complement(675..>1231)
                     /locus_tag="PKO06_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72728.1"
                     /translation="LEWGAANGIHEAVMAYIGNEGIDVLYDNNGDFAFPTPRSWEMAS
                     KVFSQGIDEEKKRLMAACIGPSAADKFFTFQKIYGQVDVEAIVNKGLVMDFSKGRKNE
                     PSFVYAATFAVAAYASNLPTLQPAQASNVVKFLRSPGLDPEYVFLFLRQIKHRGKNFD
                     CLKPVPDFQALAGSLVDIRSKMYK"
BASE COUNT          232 a          324 c          382 g          293 t
ORIGIN      
        1 tgatgatcga gcgtgttggg cagttcgacg agttcttctc ctgggtcatt gtccgagttg
       61 tcgccaccgg agccggcatc ttcatcgtcg tgctgacccg gttgttttgg catgcgcaga
      121 gtgagtttga tgatcgacgg cttctgcagt tcgtcttccg cgagtcgctc gtagatcatc
      181 tcggcgatca tgccgtcgta ctgtttgtcg agcagaatct tgatctcttc gccgttctcc
      241 agtttggtat tcggcacctc atagagcgga cgtttctgcc agccgcccgg gtggcagatt
      301 tcagcgacga tgcgattgat ggcgtagtcg gtggcgcagt tccagagaaa cggattgcga
      361 actccctgcc gttcgttcgt ggcaaagacg ctgtgaccga tctcatgcgc caggacaaac
      421 cccagttgtc gaatgttcaa ctgccgcgtc tgcagaggat tgtaccagat ggtgcgatag
      481 cagtcggtgg cggccagatg cggcagagag acatcgggaa tcaggcgcat ctgcagcagc
      541 agataccccc agaacgggtg ggtctcgagc agttgcgaac gaatggaggt cagccgttcg
      601 cgttcatctc gaatgacctg ttcgttgtcg agagctgtat tcgtctcgct gttcatgggt
      661 gtgtccttta tggatcactt gtacattttc gatcggatat cgaccaggct accggccagc
      721 gcctgaaaat cgggcaccgg cttgaggcaa tcgaagtttt tcccccgatg cttgatttgc
      781 cgcaggaaca gaaagacata ctcggggtcg agtccgggcg agcgcaggaa cttgaccaca
      841 ttcgaggcct gggcgggttg cagggtcggc aggttggagg cgtaggcggc cacggcgaag
      901 gttgcggcgt agacgaacga gggctcgttc ttgcggcctt tggagaaatc catgaccagg
      961 cccttgttga cgattgcttc aacgtcgacc tgaccgtaga ttttctgaaa ggtgaagaac
     1021 ttgtcggcgg ccgaaggccc gatacaggcg gccatgaggc gtttcttctc ttcgtcgatg
     1081 ccctgcgaga agaccttgct cgccatttcc catgagcgcg gggtgggaaa ggcgaagtcg
     1141 ccgttgttgt cgtagagaac atcgatgccc tcattgccga tataggccat cacggcttcg
     1201 tgaatgccgt ttgccgcgcc ccactcgagc c
//
LOCUS       DAORHD010001584         1230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162302_length_1230_cov_3.014468, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001584 DAORHD010000000
VERSION     DAORHD010001584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162302_length_1230_cov_3.014468"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..>1230
                     /locus_tag="PKO06_23670"
     CDS             31..>1230
                     /locus_tag="PKO06_23670"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV72729.1"
                     /translation="MSELDGEQKKGLAAKLPDILAILASLAVFALAWLVFTNKIPKLY
                     RWLYQHFYVLALVGVLGFLGEKISVVIMIFVLPVLLVGLTVGIKFRPEKFPVEDWWDI
                     YALMGVLAAGFCVYLFYRWEETKKMKGLEGGGPLSGRVDPRAAAKYREEQARKDASAN
                     KLDGIKDDESKVKAKKKEEKVAEVVDPNETAEQRFEREMRQMKEEFSRKSMRLSTTLL
                     RMKNLAKSLDRDEIFSTVLEIIAKGMDATRIQLLLMDDKEHKLRVVRAEGMPAKEYKD
                     MVIPMEENSILGYLARVKDTKTLEGGSLGVKEAELDPKTKGLGNAGTLKTVLAAPILV
                     DGKVFGVINVEKMRNPDYTRDDQALIATCADVAGLVMKNAKLYAATMDDLVSTKKLSE
                     EQLKQNEQ"
BASE COUNT          318 a          299 c          367 g          246 t
ORIGIN      
        1 aaaagggtaa aatagaagag agagggctgt atgtcggaac tggatggcga gcaaaagaag
       61 gggttagccg cgaaactgcc cgatattctg gcaattctcg ccagtctggc agtgttcgct
      121 ctcgcctggt tggtgttcac caacaagatc cccaagctgt atcgctggtt ataccagcat
      181 ttctatgtgc tggcgctggt cggagtgctc ggcttcctcg gcgagaagat ctcggtggtc
      241 atcatgatct tcgtgctgcc tgtactgctg gtcgggctga ccgtcggcat caagtttcga
      301 ccggaaaaat tcccggttga agactggtgg gacatctatg ctctgatggg cgttctcgcc
      361 gcgggttttt gcgtctacct gttctatcgc tgggaagaaa ccaaaaagat gaagggcctc
      421 gagggcggag gtccgctttc cgggcgggtc gacccacgtg ccgccgccaa ataccgtgag
      481 gagcaggccc gcaaggatgc ctcggccaac aaacttgatg gcatcaagga cgacgaatcg
      541 aaagtcaaag cgaagaagaa ggaagagaag gtcgcggaag tcgtcgatcc gaacgaaaca
      601 gccgaacagc gtttcgaacg tgaaatgcgc cagatgaaag aagagttcag tcgcaagtcc
      661 atgcgtctgt cgacgacgct gctgcgcatg aaaaatctcg cgaagagtct tgaccgcgat
      721 gaaatctttt cgaccgtgct tgaaatcatc gccaaaggca tggatgccac gcggattcag
      781 ctgctgctca tggacgacaa ggagcataag ctgcgcgtcg tgcgcgccga agggatgcca
      841 gccaaagagt acaaggacat ggtcattccc atggaagaaa acagcattct ggggtatctg
      901 gccagggtga aagatacgaa aaccctcgaa ggaggctcac ttggggtcaa agaagccgag
      961 cttgatccga aaacgaaagg gttgggcaac gccggcacgt tgaaaaccgt tcttgccgcc
     1021 cccattctcg ttgatggcaa agtcttcggg gtcatcaatg tcgagaagat gcgcaatcct
     1081 gactatacgc gcgatgatca ggcattgatt gcgacctgtg ccgatgtcgc cgggctggtt
     1141 atgaagaatg ccaagttgta cgcagcaacc atggatgacc ttgtttcgac gaaaaaactc
     1201 agcgaagaac agctgaagca gaacgagcag
//
LOCUS       DAORHD010001585         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162654_length_1229_cov_1.994037, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001585 DAORHD010000000
VERSION     DAORHD010001585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162654_length_1229_cov_1.994037"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..>1229
                     /locus_tag="PKO06_23675"
     CDS             221..>1229
                     /locus_tag="PKO06_23675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV72730.1"
                     /translation="MQEQHDAANEELQVSSEEVQSANEEFQSVNEELETSKEELESAN
                     EELITVNEEMNHRNSELNVLNNDLINLQTSTKLAIVLLGRDLSIRRFSPQAEKMFDLL
                     ATDVGRPVGHIRHRLVSAPVSNGQRGEGNGSASQTLAMAPTDLEAIAAEVIVSEQEYD
                     CDAQDQARNWYSLRVHPYKSNDGKVNGAVLVLVDITDRKHAAEIVAEYQSRFHQMIDA
                     LPVAVYVTDVQGCLTHFNRVAVQFSGQTPVLGVDKWYVNCKLFHPDGTPMAYDTSPMI
                     HAIKGGKVTPGQTVIVERPDGTRVWGDVYPMMLRSNSGNIIGAINVVVDVTERRQLEE
                     SR"
BASE COUNT          312 a          323 c          343 g          251 t
ORIGIN      
        1 cgaacaaggc agcccgaccc tggtgaatct tgaagtcatc ccgctgaaaa acctgcgtga
       61 acggtgtttt ctggtcatct ttgaagaacc agctcgtatc agcggaccgg tggcaaagaa
      121 cctccgacca ccgttatctg aacaggagca ggcagaagaa cgtggccgcg tttccgaact
      181 cgaaacggat ctctcagaga ttcgcgagtt cctgcaagtg atgcaggagc aacacgacgc
      241 ggcgaacgaa gaacttcagg tttccagcga agaggttcag tctgcaaacg aagaatttca
      301 gtcggtgaat gaagagctgg aaacttcgaa ggaagagctg gagtcagcga acgaagaact
      361 catcacggtc aatgaggaga tgaaccatcg caactccgag ctcaatgttc tcaacaacga
      421 cctgatcaat cttcagacgt cgacaaagct tgccatcgtg ctgcttgggc gtgacttgag
      481 tatccgtaga ttcagcccac aagccgagaa aatgtttgac ctgctcgcga ctgatgtggg
      541 caggcccgtt ggccacattc gtcaccgcct tgtctctgcg cctgtgtcga acggccagcg
      601 aggggagggg aatggttcag cctcacaaac actggcaatg gctccaacag atctggaagc
      661 tattgccgca gaagtcattg tttcggaaca ggaatatgac tgtgatgccc aggatcaggc
      721 acgaaactgg tactctctcc gcgtccatcc gtacaagtcc aatgatggca aggtcaacgg
      781 cgctgtgctt gtactggttg acatcaccga tcgcaagcac gcggcggaaa tcgtggcgga
      841 ataccagtcg cgattccacc agatgatcga tgcgcttcct gtggcggtgt atgtgactga
      901 tgttcagggc tgtctcacgc acttcaatcg cgttgccgtc caattttccg ggcaaacgcc
      961 tgtgctggga gtcgacaagt ggtatgtaaa ctgcaaattg tttcaccctg atgggacgcc
     1021 catggcgtac gacacaagcc ccatgataca tgcgatcaaa ggaggtaaag taactccggg
     1081 tcagaccgtg atcgtcgagc gccccgacgg gactcgggtt tggggtgacg tataccccat
     1141 gatgcttcgc agcaacagtg gcaacatcat cggcgccatc aacgtagtgg tagacgtcac
     1201 cgagcgcagg caacttgagg aatcacgac
//
LOCUS       DAORHD010001586         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162781_length_1228_cov_2.963342, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001586 DAORHD010000000
VERSION     DAORHD010001586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162781_length_1228_cov_2.963342"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PKO06_23680"
     CDS             <1..308
                     /locus_tag="PKO06_23680"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV72731.1"
                     /translation="DSPVTPLDPLLGLHGFINHPNEAERIDLNTALAAFILEPHRFAG
                     TDSDRGYLRPGYLANFVCLSANPFLVRPSELRDIQVTHLYIDGRNVYPTSPTPRGSK"
     gene            305..940
                     /locus_tag="PKO06_23685"
     CDS             305..940
                     /locus_tag="PKO06_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72732.1"
                     /translation="MKLSVSFLLLIAVLFSTGLYAQPVPLETTPNPQVREIQTVVNEI
                     FPLLIKKDSEGFLKKLFEVFPIGEQARISFSNDVKHLIARLQSPLDFEFVGYRMSGQS
                     VRFIQVYYLTIHPTMPLGWEFTFYRPVADGPWQMNFLRYHSDELYEFLNYPKLAFEVM
                     QKTLREELQGPRPTISDVTDFNRIPNRPLPSEVASSPTPAPAGSESPAAPK"
     gene            1078..1228
                     /locus_tag="PKO06_23690"
                     /pseudo
     CDS             1078..1228
                     /locus_tag="PKO06_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ia"
BASE COUNT          263 a          373 c          300 g          292 t
ORIGIN      
        1 cggacagtcc cgtgacccca ctcgacccgc tccttggttt gcatgggttc atcaatcatc
       61 cgaacgaagc cgaacgcatc gatctgaata ccgcgttggc tgccttcatt ctggaaccac
      121 atcgatttgc gggtaccgac agcgaccgtg gctatctgcg acccggatat ctggccaact
      181 ttgtctgtct ctcagccaat ccattcctcg ttcgcccttc tgaattgcga gacattcaag
      241 tcacacactt gtacattgat ggacggaacg tctatccaac atcccccacc ccaagaggat
      301 caaaatgaaa ctgtctgtct cttttctgct tctgatcgcc gttcttttct caactggctt
      361 gtacgctcag cccgtgccgc tcgaaacgac gcctaatcca caggtcagag aaattcagac
      421 tgtggtcaac gagatctttc cccttctgat caaaaaggat tccgagggct ttctgaagaa
      481 actgttcgaa gtgttcccga ttggcgaaca agctcgcatc agtttcagta acgatgtgaa
      541 gcatctgatc gcgcgactgc aatcacctct tgatttcgaa tttgtcgggt atcggatgtc
      601 cgggcagtcg gtgcgtttca ttcaggtgta ctatctcacg attcacccca ccatgccgct
      661 cggctgggag ttcacgttct atcgtccggt cgcagatggc ccctggcaga tgaacttcct
      721 gcgctaccac tcggacgaac tctacgaatt tctgaactat ccgaagttgg cctttgaagt
      781 catgcagaag acattgcgcg aagagttgca gggtcctcga ccgaccattt ccgacgtgac
      841 cgatttcaac cggattccga atcgaccact cccctcggaa gtcgcttcct cccccacccc
      901 ggcgcccgcg ggctctgaaa gccctgccgc accaaagtaa gcatcgccac gtcggtcggc
      961 ttttcgtcac aggatccgat gggatatacg ttccgcccga tcgctgagtc tccggatctt
     1021 cgcgatcagg cggcaaacat tcttcacacg acgttcgcga gccttggcaa cacctgctgg
     1081 cctgatctgg acagcgcgat gaaagaagtt gtcgagtgcc tcgaagcgcc gaacctctgc
     1141 atcggaatct gcgatgacgg agagctgctc ggctgggtcg ggctgagacc aatgtatgcc
     1201 agaacctggg aactgcatcc actggtcg
//
LOCUS       DAORHD010001587         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_162862_length_1228_cov_2.050298, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001587 DAORHD010000000
VERSION     DAORHD010001587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_162862_length_1228_cov_2.050298"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..103
                     /locus_tag="PKO06_23695"
     CDS             <1..103
                     /locus_tag="PKO06_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HNV72733.1"
                     /translation="PVMSGARLFLAEQIGQVIKNGLALLGVEAASEL"
     gene            133..1125
                     /locus_tag="PKO06_23700"
     CDS             133..1125
                     /locus_tag="PKO06_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72734.1"
                     /translation="MSGDEQNSWADQAVGAGKPFDVSAELGAAEELGERLALGEEDSL
                     APCVHRIETRFFGTRAVTHAMAPQARQHRLEAFLAAYPEADPQLIVKALHQLYLICLH
                     ELRDPVAAEGWLKKLEAMPEGADKVRVERLLQVTADRALHDDGMLEQLSALEQALAKK
                     PYDNGYVPVLEPQRLKLLVAQANLLNGQYAAVLPLVKEFSEDVRVRTLPVRLLYALLA
                     DEKDLAYQLFVKHEKAMIAGDRKMIDDFLYPLYLPVRENEKRIIAATALAADHPTTAV
                     KLLLEESKKPRRSMHFDHAFALLAELYQRQGSYAEAQSVWATLRRVFPHSVWTR"
BASE COUNT          257 a          345 c          369 g          257 t
ORIGIN      
        1 cccagtaatg agcggtgccc gtcttttcct cgccgaacaa atcgggcaag taattaagaa
       61 cggtctcgcc ctattggggg ttgaggccgc atcagagctt tgacacgata gttaaccggc
      121 aggggcgcga gcatgagtgg tgacgaacag aattcttggg ccgatcaagc ggtcggcgcg
      181 ggcaagccgt tcgacgtctc ggcggaactg ggcgctgccg aagaacttgg cgaacgtctg
      241 gcgctgggcg aagaagactc gctggctccg tgcgtgcacc gcatcgagac tcgcttcttc
      301 ggaacccgcg cggtgacaca tgccatggct cctcaggcgc gtcaacacag actcgaagcc
      361 tttctggccg cctatcccga agccgaccca cagctgatcg tgaaagcgct gcatcagctc
      421 tacctgatct gtctgcatga gctccgtgac ccggtggccg ctgaaggctg gctgaagaaa
      481 ctcgaagcca tgcccgaggg agccgacaag gtgcgtgttg aacgtctgtt acaggtaacc
      541 gctgatcggg ccctgcatga tgacggaatg ctcgagcaat tgtctgcgct cgaacaggct
      601 ctggcgaaaa aaccgtatga caatggatac gtgcccgtac ttgaaccgca gcggctgaaa
      661 ctgctcgtgg ctcaggccaa tctgctgaat gggcagtatg cggcggtgtt gccgctcgtg
      721 aaagagtttt cggaagacgt acgcgtgcgc acccttcccg tgcgtttgct ctacgcactg
      781 ctggcggatg aaaaggacct ggcctatcaa ctgtttgtca agcacgagaa ggccatgatt
      841 gccggcgacc gcaagatgat cgacgacttc ctgtaccccc tgtatctgcc cgtgcgtgaa
      901 aatgaaaagc gaatcattgc ggcgaccgcc ctcgccgccg atcatccgac aaccgcggtc
      961 aagctgttgc tggaagaatc gaaaaaaccg cgacgttcga tgcacttcga tcacgcgttc
     1021 gctttgctgg ctgaattgta ccagcggcaa ggatcgtatg ctgaggctca gagcgtctgg
     1081 gcgactttgc gacgggtgtt ccctcattcc gtctggacgc ggtagaagac tgacgcacct
     1141 cgtccgttgc caattcggga ggccgaagat gaagcgtatt ctgtttctgt gcctgtttct
     1201 tgcaggatcc atgctgtcag gattggtc
//
LOCUS       DAORHD010001588         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163210_length_1226_cov_3.005124, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001588 DAORHD010000000
VERSION     DAORHD010001588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163210_length_1226_cov_3.005124"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PKO06_23705"
     CDS             complement(<1..932)
                     /locus_tag="PKO06_23705"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV72735.1"
                     /translation="MTSTRNRTIIVGTGIAGTSAAFAMRDAGYTGELTLIGEEPWPAY
                     YRTRLPEMVSGEVTVDKLLVNTPAQYEEKRIRVLVNRPVHTVDADRREVQFADGARQA
                     FDQLLIATGCRPFVPPLPGAEGRAEIFALRSAQDATRIQQFARGRRHAVVVGGGLLGL
                     EAAFHLTKLGLSVEVIETAPRLMPRQLDRRSSQLLKERLEKRGFTFLVGAKANRFSAE
                     CCGGGTSLELIGGHLCQGDLYLISTGVVPRRELVDPLGLACERGIHIDPNGQTSVEGV
                     FAAGDNVQFENRIWGTWMAARHWGLRAGKAMAGQ"
BASE COUNT          238 a          356 c          370 g          262 t
ORIGIN      
        1 tcctgcccgg ccatcgcttt gccagctcgc aggccccagt ggcgggccgc catccaggtg
       61 ccccagatgc gattttcgaa ctgtacgttg tccccggcgg caaaaacccc ttcgaccgac
      121 gtctggccat tggggtcgat gtgaatgcct cgttcgcagg cgagtccgag cggatcgacg
      181 agttctcgac gtggaacgac ccccgtcgag atcagataga gatcaccttg gcagagatgc
      241 ccgccgataa gctcgagcga agtgccgccc ccgcaacact cggcgctgaa acggttcgct
      301 ttggcaccga ccagaaaggt gaaaccgcgt ttctcgaggc gctctttcaa caactgcgac
      361 gagcgccggt cgagttggcg gggcatcagg cggggagcgg tttcaatcac ctccaccgaa
      421 agtccgagtt tggtcagatg aaatgccgct tcaagaccga gaagaccgcc gccgacaacc
      481 accgcatgcc gccgtccgcg agcgaactgc tgaatgcgcg tcgcatcctg tgccgagcgc
      541 agggcgaaaa tctcagcgcg gccttcggct ccgggcagcg ggggaacgaa gggtcgacac
      601 ccggttgcga tcaacaattg gtcaaacgct tgacgcgctc catcggcaaa ctgaacttcg
      661 cgacggtcgg cgtcgacggt atgtaccggt cggttgacca gcacgcgaat ccgtttttcc
      721 tcatactggg ctggggtgtt gaccagcagc ttgtcgacgg tcacttcacc tgagaccatc
      781 tcgggcagtc gggtgcgata atacgcgggc cagggttctt cgccgataag cgtgagttct
      841 cccgtatagc cggcatcacg catggcaaaa gccgcgctgg ttccggcgat tccggtgccg
      901 acgatgatcg tgcgatttcg cgttgaggtc atggcaatct cctctcgatt tcctctcatt
      961 acgatagctg ctctcgccac gtgaagcaag tctcacaact attcaggcga tactgatttt
     1021 gatatggtag gctgtgcgtt gctgtgcaga tgagggggga agatgacatc ccggacttca
     1081 gacgacaacc cgttgaaagg cgttcccgat tgggtcgtca tgttcgttgc ctggttggtt
     1141 ttgaattcga gactgtacga gtggcatctc gattttcttc tggttgcggc aggtatcggt
     1201 tacgtcggtc tcgatgtgat gcgcac
//
LOCUS       DAORHD010001589         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163215_length_1226_cov_2.976089, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001589 DAORHD010000000
VERSION     DAORHD010001589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163215_length_1226_cov_2.976089"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="PKO06_23710"
     CDS             complement(<1..364)
                     /locus_tag="PKO06_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72736.1"
                     /translation="MRLELILLFLCVGQASRVLVLFPLRAQAFKEIFMGMKVELDRST
                     VLVLTYSDLIINWPILALVVVGLLLAAFFGGVRWLVESANRASLSGTIPLARFQVTAT
                     AFLTMSLIVLLSVGAYAEH"
     gene            complement(473..>1226)
                     /locus_tag="PKO06_23715"
     CDS             complement(473..>1226)
                     /locus_tag="PKO06_23715"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV72737.1"
                     /translation="MLPANGTARGDPFVSWTKPFKGQKEVSVIGPFEIGFGQPMEQAT
                     ILDDVEVNAQQEGGGPTVVLNGSQLARLFNLEWVTPTVLRFTPKIPLAPLARYSMLVT
                     KWNARAADGRILRNYTGMFGSFTTGPDNAPSVKSTSPRNGEMNVGRSGPFTVTFDRSM
                     DPTTLTTDLRLRLTHVDSGASVMIDGSTFDSLFSAVWSLDNTVLSLVPKLMMAANAKY
                     LIQIERFSLRSRTGKPMTDTSALWGQFMTGAL"
BASE COUNT          284 a          333 c          371 g          238 t
ORIGIN      
        1 cgtgttcggc ataagcgccg accgagagca gcacgatcag ggacatggtc aggaatgccg
       61 tggcagtgac ctgaaaacgg gccagaggaa tcgttccgct cagactggcg cgattggctg
      121 actcgaccag ccagcgcaca ccgccgaaga atgcggccag aagcaggccg acaacgacca
      181 gcgccagaat cggccaattg atgatcagat cagagtaggt gagcaccaga acagtgctgc
      241 gatcaagttc caccttcatg cccataaaga tctccttgaa cgcctgggct cgcagcggaa
      301 acaacacaag cacgcgcgaa gcctggccga cgcacagaaa caggagaatc agttccagac
      361 gcaccagcgc cagaatctca tagaatgtta acgacctcgg acgattgatc gtactcgttg
      421 agttttgtga ggatgttgat gaagagatgt tgtgggttgt cgaggaagac atttacagag
      481 ccccggtcat gaactgcccc cagagagccg acgtgtccgt catcggcttc cccgtgcgtg
      541 acctcaggga aaagcgttcg atctggatca gatacttggc attcgctgcc atcatgagtt
      601 tgggtaccag cgagagcacg gtgttatcaa ggctccagac ggcgctgaag agagagtcga
      661 aggtcgaccc atcgatcatg acggaggcac cactgtcgac atgagtaagc cgcagacgca
      721 gatcggtcgt cagcgtggtt ggatccatgg atcgatcgaa cgtgaccgtg aaaggacctg
      781 aacggccgac gttcatctcg ccgttgcgcg gggatgtcga tttcacgctg ggggcgttgt
      841 ccgggccggt cgtgaaactg ccgaacatac cggtatagtt gcgcagaatt cgaccgtcag
      901 cggcgcgggc attccatttg gtcaccagca tgctatatct ggccagcggt gcgagaggga
      961 ttttcggagt gaaacgtaga acggtcggtg tcacccattc gaggttgaac aggcgtgcca
     1021 gttgcgatcc attcaaaacc acggtgggtc cgccgccttc ctgttgggcg ttgacttcga
     1081 cgtcgtcaag aatcgtcgcc tgctccatgg gctggccgaa accgatctcg aaggggccga
     1141 tcaccgatac ctctttctgc cctttgaatg gtttggtcca ggatacgaac gggtcaccgc
     1201 gagccgtgcc gtttgccggc agcatc
//
LOCUS       DAORHD010001590         1226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163222_length_1226_cov_2.804441, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001590 DAORHD010000000
VERSION     DAORHD010001590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163222_length_1226_cov_2.804441"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="PKO06_23720"
     CDS             complement(<1..300)
                     /locus_tag="PKO06_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72738.1"
                     /translation="MMTQVQRSVAKTVGLSETFRRYGAVGMLRLIRDVLVSKLVLPQA
                     RIVRYPFYLRGKRHMRVGKGFTAGVGLRLDAFPSDERVCLTIGERVEVNDYVHIGA"
     gene            complement(297..>1226)
                     /locus_tag="PKO06_23725"
     CDS             complement(297..>1226)
                     /locus_tag="PKO06_23725"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV72739.1"
                     /translation="IDMKPLISVLVPVYNWQVSRLSQALLAARKKVYADDLIEIRFLD
                     DSSPNQSVQADNRRYFDTVSEWGVFYRELPENRGRAVVRNTLSMEAQGDWLLFLDADV
                     VPDDELFLLNYCDVIRSDQADAVCGGTSYRQRTMTGRSYDFYVYLVSRAGEKDAGSRN
                     MVPWRTVLTSNMLIKKILFETTPFDARFLGYGYEDQEWGIRLQHKCRLLHIDNTVSHL
                     GLQTKQEVYRKMRESVKNYHLLRRLHPEMFAASGIARIVRFLESGSAHCLRKLDRLLE
                     RAFWALEQPYAISYCTFQLNKAVLLALEGKREP"
BASE COUNT          302 a          367 c          299 g          258 t
ORIGIN      
        1 ggctccgatg tgcacatagt cgttgacctc gacgcgctca ccgatggtga gacagactcg
       61 ctcatcgctc gggaacgcat cgagtcgcaa ccccaccccg gccgtaaaac ccttgccgac
      121 acgcatgtgc cgtttgcccc gcagatagaa agggtatcgc acaatccggg cttgcgggag
      181 tacgagtttc gaaaccagca catcgcgaat caaacgcagc atgccaactg ctccatatcg
      241 ccgaaaagtt tcactcaacc cgacggtttt cgccacactt cgttggacct gcgtcatcat
      301 ggctccctct ttccctcgag tgccaaaagc acggctttgt tcaattgaaa cgtgcaataa
      361 gagatcgcat acggttgttc cagcgcccag aacgcccgtt caagcaatcg gtcaagtttt
      421 cgcagacaat gcgccgagcc gctttccagg aaacgcacga ttcgggcaat gccgctggca
      481 gcaaacatct caggatgcaa ccgtctcaac aagtggtagt tttttacgga ctcgcgcatt
      541 ttacgataca cctcttgctt tgtctgcaag ccaagatgcg acacggtgtt gtcgatatgc
      601 aacaatcggc acttgtgctg caacctgatc ccccactcct ggtcctcgta tccgtaaccg
      661 agaaaacggg catcgaacgg cgttgtctca aacaggattt tcttgatcag catattcgaa
      721 gtcagaaccg ttcgccaagg gaccatattg cgcgacccag cgtctttctc gcccgcccga
      781 ctgaccagat agacgtagaa atcataggag cgcccggtca tggttcgctg acgatagctt
      841 gtgccaccac acacggcatc ggcctggtca gatcgaatga catcgcagta gttcaagaga
      901 aatagttcgt catccggcac gacatcagca tccagaaaca acaaccagtc gccctgagct
      961 tccattgata gtgtgttgcg caccacggcg cgaccgcgat tctcaggcaa ctcacgatag
     1021 aacacccccc attcgctcac cgtatcaaaa tagcgtcgat tgtcagcctg gacactttga
     1081 ttcggcgagg aatcatcgag aaagcggatc tcgatcagat cgtctgcgta caccttcttt
     1141 cgagcggcaa gcagcgcctg gctcaaccgg gagacctgcc agttatagac aggcacgaga
     1201 acggagataa ggggcttcat gtcgat
//
LOCUS       DAORHD010001591         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163610_length_1224_cov_3.698888, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001591 DAORHD010000000
VERSION     DAORHD010001591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163610_length_1224_cov_3.698888"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1224)
                     /gene="gcvPB"
                     /locus_tag="PKO06_23730"
     CDS             complement(<1..>1224)
                     /gene="gcvPB"
                     /locus_tag="PKO06_23730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNV72740.1"
                     /translation="HFTRLSRLNYGVDVGFYPLGSCTMKYNPKINEETARLPGFANLH
                     PYQPDETIQGSLCLLKSFEDWLCELTGLDAFTLLPAAGAHGEFVGLMIIHAYHDQKKN
                     KKTKILIPTAGHGTNPASAAMCGYEVVSVGTDARGGVDIADLKAKLGPDVAGLMLTNP
                     NTLGLFEENIEEIAELVHGVDGLLYYDGANLNAIMGWCRPGDMGFDVMHINVHKTLST
                     PHGGGGPGAGPVGVKKHLEKYLPLPRVHFDGKKYKVEGANKTSIGRTRSFYGNFGVLV
                     RAYTYVCMLGGEGLKEVSTNAVVNANYMMRELQKQYHLPYDRHCKHEFVLSGKPFAEK
                     GIKTLDIAKRLLDEGYHAPTIYFPLIVDEAIMIEPTETEAKPTLDGFVKVMNRIAEEI
                     KTQPEKLKAAPVTTP"
BASE COUNT          235 a          366 c          342 g          281 t
ORIGIN      
        1 ccggagttgt caccggtgcc gctttcagct tttccggctg ggtcttgatc tcttcggcaa
       61 tgcggttcat cactttgacg aagccgtcga gcgttggctt tgcctcggtt tcggtcggct
      121 cgatcatgat cgcctcatcg acgatcaacg ggaagtagat cgtgggcgcg tgatagccct
      181 cgtcgagcag gcgcttggca atatcaagcg tcttgattcc tttctcggcg aacggcttgc
      241 ccgagagaac gaactcatgc ttgcagtgcc gatcatatgg cagatgatac tgcttctgca
      301 gttcccgcat catgtagttc gcgttcacga ccgcgttggt cgagacctct ttcaaccctt
      361 cgccgccgag catgcagaca taggtgtagg cacgcaccag cacgccgaag ttcccgtaaa
      421 acgagcgtgt gcgaccaatg ctcgtcttat tcgcaccttc gaccttgtac ttcttgccat
      481 cgaaatgcac gcgaggcagg ggaagatact tctcgagatg cttcttcacg ccaaccggcc
      541 cagcgcccgg tccgccacca ccgtgcgggg tcgacagagt cttgtgcacg ttgatgtgca
      601 tgacgtcgaa gcccatatcg cccgggcggc accagcccat gattgcgttc agattggcgc
      661 cgtcgtagta cagcaaccca tcgacgccat gaaccagttc ggcaatctct tcgatgttct
      721 cttcgaacag gccgagcgtg ttcgggttgg tcagcatcaa cccggcgaca tccggcccca
      781 gtttcgcttt cagatcagcg atgtcgaccc caccgcgcgc atcagtgccg accgagacga
      841 cttcgtatcc gcacatggca gccgacgccg ggtttgtgcc atgccccgcg gtcggaatca
      901 ggatcttggt ctttttgttc tttttctggt catgatacgc atgaatgatc atcaggccca
      961 cgaactcgcc atgagcgccc gcggcgggca gcagggtgaa tgcgtccagg ccggtaagtt
     1021 cgcacagcca gtcctcgaaa gactttagca ggcacagaga cccctgaatc gtctcatcag
     1081 gctgatacgg atgcagattg gcgaaccctg gcagccgtgc ggtttcttcg ttgatttttg
     1141 ggttgtactt catcgtacaa gacccgagcg ggtagaaccc gacatcgacg ccgtagttga
     1201 gccgtgacag ccgggtgaag tgtc
//
LOCUS       DAORHD010001592         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163660_length_1224_cov_2.731394, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001592 DAORHD010000000
VERSION     DAORHD010001592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163660_length_1224_cov_2.731394"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1224
                     /locus_tag="PKO06_23735"
     CDS             <1..>1224
                     /locus_tag="PKO06_23735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72741.1"
                     /translation="VANLTGFTRKTAHDIYGFRFGSGMANSSDFEVTASIKLREVNPV
                     KPKDGSFAIFDKKEKQGELTVTSRVNYRGRHAKITLLFLLRVVKTFIPPYNYFTLFIR
                     DGSAFGGSDWNSFDSLTGKQDVYLTLDHGWNDIITNFDATKHVADWEKALTQGQVPPG
                     RVYMGQEPQLGAPATTIQATNGAKMLVDRPTLDQSKALQYNGQDNFWLKFDVPWMLFD
                     DYRKKVTEIQGDQAIKKDFFDGIKGLFTDVFGKDVEIRVINVGAGQELTQDVAFGEPA
                     FVRAVQSYIKFRDHRLRAPGQIPFDVKQVMYPEITKSGLHIFGHAPPPSGNINPYGTA
                     DFSKLSPTIVYGQVMRQYNRIVSLRRKPGASNPPFVDPKDPSTPINLEIPFIGTEPSP
                     DGSAPPITVTFNPKSP"
BASE COUNT          278 a          358 c          323 g          265 t
ORIGIN      
        1 ggtcgccaat ctcaccggct tcacgcgcaa aacggcgcac gatatttacg gcttccgctt
       61 cgggtcggga atggcgaact ccagcgattt cgaagtaacc gcatcgatca aactgcgtga
      121 agtcaatccg gtcaagccca aagatggatc attcgccatc ttcgataaga aagagaagca
      181 gggcgaactc accgtaacct cgcgggtcaa ctatcgcggg cggcatgcca agatcaccct
      241 cttgtttctg ctgcgtgtcg tgaaaacgtt tattcctcca tacaactact tcaccctctt
      301 tattcgcgat gggtccgctt tcggtggctc agattggaac tcgttcgatt cgctgaccgg
      361 caagcaggat gtctacctga cactcgatca cgggtggaat gacatcatca ccaacttcga
      421 tgccaccaaa catgttgccg attgggagaa ggccctgacg caggggcagg taccgcccgg
      481 ccgtgtctac atgggccagg aaccgcaact cggggcgccg gcgaccacca ttcaggcaac
      541 gaatggggcc aagatgctgg tcgaccgtcc gacgctcgat cagagcaagg ccttgcagta
      601 taacggtcag gataacttct ggctgaagtt cgatgtcccc tggatgctgt tcgatgatta
      661 ccgcaagaag gtgaccgaga ttcagggcga ccaggcgatc aagaaagatt tctttgatgg
      721 catcaagggg ctgtttaccg atgtcttcgg gaaagacgtc gagattcgcg tgatcaatgt
      781 tggtgccgga caggaattga cccaggatgt cgcattcgga gagccggcgt tcgtcagagc
      841 ggttcaatcg tacatcaagt tccgcgatca ccgtttgcgc gccccggggc agattccctt
      901 cgacgtgaaa caagtgatgt atccagagat tacgaagagc ggtctgcata tcttcgggca
      961 tgccccgccg ccgtcgggaa acatcaaccc ctatggcacc gctgatttca gcaaactttc
     1021 tcccaccatt gtctatgggc aggtcatgcg tcagtacaac cgcattgttt ctctcaggcg
     1081 caaacccggg gcgagtaacc ccccgtttgt cgacccgaag gacccgagca cccccatcaa
     1141 tcttgagatt ccgtttatcg gcaccgaacc ttcgcccgat ggttctgccc ctccgattac
     1201 cgttaccttc aaccccaagt cgcc
//
LOCUS       DAORHD010001593         1224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163688_length_1224_cov_2.408041, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001593 DAORHD010000000
VERSION     DAORHD010001593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163688_length_1224_cov_2.408041"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1224
                     /locus_tag="PKO06_23740"
     CDS             <1..>1224
                     /locus_tag="PKO06_23740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HNV72742.1"
                     /translation="RLREIVLTGLLALPTAVLAMVIDAGRLGLYLQMLCSGLILLTSG
                     RRFFRSGWQAMKNRSPNMDVLVALGVGTSWGYSALMVVQPHLAHDGMAHFEGAALLVF
                     FILVGKGLESWARRLASGELRRLVDLQQRDVRVCDDGVEHLVALDAVKIGQQVRVLPG
                     ERFPTDAIVVEGASAADESLLTGESLPVEKATGSVVLSGSVNLQAPLRVKVTRDGAES
                     TLQTLIRAVRRAQADKPPIQRFADRAAEWFVPVIIGLSCLTALGWWLAGAPVSLAIMH
                     AVTVLVVACPCALGLATPTAVVVACGMAMRHGLLVRRPSALETLAQVRYLLLDKTGTI
                     TQGRPQVQVFHACDEALTCEERGVVAALAGSSNHPLSTTIRAWATETSLSSVPLTEVS
                     EQRGLGLAARDSEGMW"
BASE COUNT          176 a          349 c          421 g          278 t
ORIGIN      
        1 cgtctgcgtg agattgtcct gaccggtctg ctggccctgc cgaccgcggt tctggccatg
       61 gtaatcgatg ccggtcgtct ggggttgtat ctgcagatgc tctgttccgg cctgattctg
      121 cttaccagtg gtcgccgatt ttttcggtct ggctggcagg cgatgaagaa ccgatcaccg
      181 aacatggatg ttctcgtggc cctgggcgtc ggaacttcgt ggggatactc ggcgctgatg
      241 gtggtgcagc cgcatctggc gcacgacggc atggctcact tcgagggggc cgcccttctg
      301 gtgttcttca ttctcgtggg caaagggctg gaatcgtggg ctcgtcggct ggcttcggga
      361 gagttgcgcc ggctggtcga tctgcagcag cgtgacgttc gagtctgtga cgacggagtt
      421 gagcatctcg tagctctcga tgccgtgaaa atcgggcaac aggtgcgagt gctccccgga
      481 gaacgttttc ccaccgacgc gatagtggtc gaaggggctt cggctgccga tgagagtctg
      541 ctgaccggcg agagtcttcc ggtcgagaaa gcgacgggtt cggtcgttct ctctgggagt
      601 gtcaatctgc aggctccgct tcgggtgaaa gtgacgcgtg atggagcgga atcgacgctt
      661 cagacgctca ttcgcgcggt tcgacgcgcg caggcggaca aacccccgat tcaacgcttt
      721 gcggaccggg cggcggaatg gttcgtgccc gtgatcatcg ggctctcctg tttgacggct
      781 cttggctggt ggctcgctgg cgcgcctgtt tcactggcga tcatgcatgc tgtcacggtt
      841 ctggtggtgg cctgcccctg tgctctgggt ctggcgacgc ccacggcggt cgtcgttgcc
      901 tgcggaatgg cgatgcgtca cggtctgctg gtgcgtcgcc catcagcgct cgagacgctg
      961 gcgcaggttc gctatctcct tctcgacaag actggaacca tcacacaggg gcgtccgcag
     1021 gttcaggtgt ttcacgcctg cgacgaagcg ctgacctgtg aggaacgcgg cgttgtggcc
     1081 gcgctggccg ggtcctccaa ccacccgctg tccacgacga ttcgagcgtg ggccaccgaa
     1141 acgtccctgt cgtccgtccc gttgaccgaa gtgtcggagc aacgtgggct gggtcttgcg
     1201 gctcgcgatt cggaaggtat gtgg
//
LOCUS       DAORHD010001594         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163818_length_1223_cov_3.651541, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001594 DAORHD010000000
VERSION     DAORHD010001594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163818_length_1223_cov_3.651541"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKO06_23745"
     CDS             complement(<1..193)
                     /locus_tag="PKO06_23745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72743.1"
                     /translation="MLGEEFLWGMNREAESPGASKRRRTVNESSSWRTKTGQGQTGGI
                     SIVSRTPDEDRTSGNQGPEG"
     gene            215..>1223
                     /locus_tag="PKO06_23750"
     CDS             215..>1223
                     /locus_tag="PKO06_23750"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72744.1"
                     /translation="MIASGFFADEIAEACQKSGWQRYRDFVVYGDLRRFNYQVEVAGM
                     CNLKCISCPRGNWPRHRKPGLMSLDSYRALVDKILTDDPWTGIITLYNWGEPLLNPAL
                     PDIIRLTREKGLLSAVSSNLAMQKDYEEVVKAGPDWFRISNSGWGANYETTHTGGKWD
                     LFLANCRKLAKYRAQHSPTMIVEFFFHIYAHNREDFPKMQQLCDELGFTLRYRHAALA
                     PLDNVSLVDGGQTVSDAAARTREIQFLHVEEVMEIARREKERPCFYLNHLWIDWDRSV
                     SHCMEWYDPSLVLFPDFLAVSLDQIVAARENSTHCRDCMARGIHRAYCVYGDEKLIAA
                     KS"
BASE COUNT          261 a          353 c          321 g          288 t
ORIGIN      
        1 agccctcagg gccttgattg ccggacgtcc tgtcttcatc tggggtgcgc gacacgatgg
       61 atatgccgcc cgtctggccc tgacccgttt tggttcgcca gctgctggat tcattgacag
      121 ttcgccggcg cttcgaggca ccagggcttt cggcctcacg attcattccc cacaggaatt
      181 cttcgcccag cacacctcag agcaagcatt tatcatgatc gcctcaggtt tttttgccga
      241 cgaaattgcc gaggcatgcc agaaatccgg ctggcagaga taccgggatt ttgtggtgta
      301 tggagatctt cgccgattca actatcaggt cgaagttgcc ggcatgtgca atctgaagtg
      361 tatctcctgt ccgcgcggga actggcctcg gcaccggaaa cccggactca tgtctctcga
      421 ctcgtaccgc gctctcgtcg acaagattct gaccgatgat ccatggacgg gaatcatcac
      481 cctctacaac tggggtgaac cgctgctcaa tcccgcactc cccgacatta ttcgactcac
      541 gcgggagaaa ggactgttgt cggctgtttc cagtaatctt gcgatgcaga aagattatga
      601 agaagtcgtg aaggctggcc ccgactggtt tcgcatctcg aattcagggt ggggagccaa
      661 ctatgagacg acgcatactg gtggcaaatg ggacttgttt cttgcaaact gccgcaagtt
      721 ggccaagtat cgcgcacaac acagtcccac catgatcgtg gaattctttt tccatatcta
      781 tgctcacaat cgcgaagatt tcccgaagat gcagcaactc tgcgatgaac tcggattcac
      841 gctgcgctat cgacatgccg ccctggcccc gctcgacaac gtctcgctgg tcgacggcgg
      901 gcaaaccgtt tcagacgctg ctgcccgtac gcgcgaaatt cagtttctgc acgttgaaga
      961 agtgatggaa attgcgcgac gagagaaaga gcgcccctgt ttctacctca accatctctg
     1021 gatcgactgg gatcggtcgg tttcacactg catggaatgg tacgatccat cgctcgtcct
     1081 ctttcccgac tttcttgctg tctcgctcga tcagattgtg gcagcgcggg aaaacagcac
     1141 gcattgccgc gactgtatgg ctcgcggtat ccatcgagca tactgcgtct acggagacga
     1201 gaaactgatt gccgccaaat cca
//
LOCUS       DAORHD010001595         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_163835_length_1223_cov_3.334760, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001595 DAORHD010000000
VERSION     DAORHD010001595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_163835_length_1223_cov_3.334760"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /gene="cas1"
                     /locus_tag="PKO06_23755"
     CDS             complement(<1..639)
                     /gene="cas1"
                     /locus_tag="PKO06_23755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNV72745.1"
                     /translation="MQLILNTYGTTLRVKDGLFLVLIGDRQQEIAPRKVSSIVLTTGV
                     FMSTDVVKLAVEHNIDLLFLDKFGDPIGRVWHDRPGSITTIRRKQLSLDRTAEGLEFV
                     KEWIEAKLDNQISLLEKLRKTRPKHSSAITQAISDLEGHRQSLADIQGLPDGNRDRVM
                     LVEAHAAKVYFSTLSSLLAEKYQFSGRSRNPAQDEFNCLLNYAYGVLYGKVER"
     gene            complement(659..>1223)
                     /locus_tag="PKO06_23760"
     CDS             complement(659..>1223)
                     /locus_tag="PKO06_23760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72746.1"
                     /translation="SCVFSFFSFVTPSTAQDWERWLRTQPYTPPQFDRTPHMHPAPPG
                     KVFPFHQESRSNNLKRFLRQLRREQAVASLTEEDIGPTLSEVRAIEDQLLEAALQAEI
                     NSRTQSLAIQTEPFPESTSGVVASPSDTLVASPTLSDTATAPIVAQNPLASPSIFVPA
                     ATASVKLTMPASVPIFPGSYSAPADNE"
BASE COUNT          268 a          309 c          380 g          266 t
ORIGIN      
        1 ccgttcaacc tttccgtaga gaacaccgta ggcgtaattc aacaggcagt tgaactcgtc
       61 ctgggcaggg ttgcgactgc ggccgctgaa ctggtatttc tccgcgagca agctcgaaag
      121 ggttgaaaaa tacacttttg ccgcgtgagc ctcaaccaac atcacgcgat cgcggttccc
      181 atcgggaaga ccctgaatgt cagccaagct ttgccgatgc ccttccagat cactgatggc
      241 ctgcgtgatt gctgaagagt gcttcggacg tgttttgcgc agtttttcaa gcaaactgat
      301 ttgattgtcc agtttcgctt cgatccattc cttgacaaat tcaaggcctt cagctgttcg
      361 atcaagtgag agttgtttgc ggcgaatcgt ggtgattgag ccggggcggt cgtgccagac
      421 acgaccaatc ggatcgccga atttgtccag gaagagaaga tcgatattgt gttcgaccgc
      481 gagcttgacg acatcagtag acataaagac accggttgtc aggacgatgg agctgacctt
      541 cctgggcgcg atctcctgct ggcggtcgcc gatgagaacg agaaacagtc cgtctttgac
      601 acgcaaagtc gtgccgtacg tgttgagaat caactgcatg gcttacgtgt cactcgctct
      661 actcattgtc ggcgggggcg gagtaggagc cgggaaagat ggggacgctc gccggcatgg
      721 tgagcttcac tgacgccgta gcagctggga caaaaatcga cggcgaagcg agaggattct
      781 gcgcgacgat tggggcggtc gccgtatcag aaagagtggg tgaggccacg agggtgtctg
      841 agggcgaagc gacgacaccg ctcgtcgact cggggaaagg ctcggtctga atcgccagac
      901 tctgcgtgcg gctgttgatc tcagcctgca gagctgcttc gagcagttgg tcctcgatgg
      961 ccctgacttc ggacagcgtt ggaccgatgt cttcttccgt gagcgaggcg acggcctgtt
     1021 cacgtcgaag ctgccgaaga aagcgtttca gattgttcga acgtgattcc tgatgaaagg
     1081 gaaagacctt tcccggtgga gccggatgca tgtgcggcgt gcgatcaaac tgcggcggcg
     1141 tatagggctg ggtgcgcagc cagcgttccc aatcctgggc agtactaggt gtaacgaacg
     1201 aaaagaagct gaacacgcag ctc
//
LOCUS       DAORHD010001596         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_164398_length_1221_cov_2.402230, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001596 DAORHD010000000
VERSION     DAORHD010001596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164398_length_1221_cov_2.402230"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PKO06_23765"
     CDS             complement(<1..590)
                     /locus_tag="PKO06_23765"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNV72747.1"
                     /translation="MSGTIPLMASVPASLAVELASKPISATDVLLFSPTDLATSPFLT
                     APDPISTSIHLLSSLFFVIVLIFGVSWLVQKKAGLGRSTFGKVLGILPLDSKRFIYVT
                     DVLNRVLVLGVTEQQITLLCEVTDKATIDALRLSQQSASAPGLESVFGFLKRKRSEEI
                     TPEDLTRHTENIQNNRQRIQDLLLKNQEKEHDLSVRR"
     gene            complement(667..1032)
                     /locus_tag="PKO06_23770"
     CDS             complement(667..1032)
                     /locus_tag="PKO06_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72748.1"
                     /translation="MPGKSILIVDDAAFMRMMIKDILTKNGYTVLGEAQTGKEAVELY
                     QKLKPDLVTMDITMPEMNGIDAVKAIKGLDPMARIVMCSAMGQQAMVIDAIQAGARDF
                     IVKPFQPARVLEAVQKALR"
     gene            complement(1051..>1221)
                     /locus_tag="PKO06_23775"
     CDS             complement(1051..>1221)
                     /locus_tag="PKO06_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HNV72749.1"
                     /translation="ELGIGSVVELNKLAGEAVEVYVNSKLIAKGEVVVIDENFAVRIT
                     SIVNPQDRLQAI"
BASE COUNT          281 a          304 c          336 g          300 t
ORIGIN      
        1 cgccgtactg acaggtcgtg ttccttctcc tggttcttca gcagaagatc ctggatgcgc
       61 tgacgattat tctgaatgtt ttctgtgtgt cgcgtcaggt cttcgggagt gatttcctcg
      121 cttcgtttgc gcttcagaaa gccgaatacg ctctcgaggc ctggggccga agcgctctgc
      181 tgactgagac gcagggcatc tatggtggcc ttgtcggtca cttcgcagag cagcgtgatc
      241 tgctgctcag tcacaccgag aaccagcact cgattgagaa cgtcggtgac gtagatgaat
      301 cgtttgctgt cgaggggaag aattccgagc acttttccga atgtggaacg cccgagtccg
      361 gctttctttt gtaccagcca tgaaacgcca aaaatcagaa ctatcacgaa aaagagggag
      421 gaaaggaggt ggattgaagt cgagatgggg tcgggagcgg tcaggaatgg tgaggtggcc
      481 aggtcggttg gcgaaaagag aagaacatca gtcgcggaaa tcggcttaga tgccaactcg
      541 accgcaagac ttgctggtac agatgccatc aaaggaatgg tgcccgacat gaccgaaaaa
      601 ctcagttaaa agcacattcg ccccggtggg gcgaatgtga aacagcaaaa ggtgaagtgg
      661 tacagttcaa cggagagctt tctgaacggc ttccagaacg cgtgcgggtt ggaagggttt
      721 cacgataaaa tcgcgagcgc ctgcctgaat ggcgtcgatg accatggctt gttgccccat
      781 ggcgctgcac atgacgatgc gagccattgg atcaagacct ttgatggctt tcaccgcatc
      841 aataccgttc atttccggca tggtgatgtc catggtgact agatcgggct tcagtttctg
      901 atacagctcg acagcttctt tgccggtctg agcctcgccc aagacggtgt agccattctt
      961 ggtaagaatg tctttgatca tcatccgcat gaacgcggca tcgtcgacta tgagtatact
     1021 cttgcctggc atgacttttc gtccgtctcc ttaaatcgcc tgcaggcggt cctggggatt
     1081 tacgatgctg gtaatgcgaa ccgcgaagtt ctcatcgatt accaccactt ctcccttggc
     1141 gatcagtttg ctattgacgt atacctccac tgcttctccg gcaagcttgt tcaactctac
     1201 aacagaacct atacctaatt c
//
LOCUS       DAORHD010001597         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_164753_length_1219_cov_4.227663, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001597 DAORHD010000000
VERSION     DAORHD010001597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_164753_length_1219_cov_4.227663"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKO06_23780"
     CDS             complement(<1..397)
                     /locus_tag="PKO06_23780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72750.1"
                     /translation="MNISSMSSTQSLWKFQMQVGSAPASAPSSPKASGIDKAAEELLS
                     VLGQTPRGASAGASETAAPAESTRPAVPPLDLSGGRIALGLTASSSYDSNSFQFSKED
                     LTALKQKIEKSGQAMSDGLSKLIDQFATTD"
     gene            complement(445..>1219)
                     /locus_tag="PKO06_23785"
     CDS             complement(445..>1219)
                     /locus_tag="PKO06_23785"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV72751.1"
                     /translation="DATTRNQLEEHLATCERCRHDRDLIQKALQVPSESLEPVRPRRD
                     LWPQIERRIQEELARKASGQTGTPVPADQSAIASPADAHRRTATRRNTYLLRLLIIAL
                     LAMSWALYQRQSRPATSPSTTVPVQSRPETQYETEFVSSDLRSIEWLLLSPDAPAFET
                     QLKLEPMQKEHLDNYRRAFTTARATRESHMSAACRGLQQLRAHQSPDKEELSKQQKRV
                     KELSRSLFDARLQEVLRIRDLLNPDQRATLLAHFNSLSR"
BASE COUNT          226 a          318 c          405 g          270 t
ORIGIN      
        1 tgtcggtggt cgcaaactgg tcgatcagtt tggacaaacc atcagacatg gcctggccgg
       61 atttttcgat cttctgcttg agtgcggtca ggtcttcttt cgagaattgg aatgagttgg
      121 aatcgtaaga gctcgaggcc gtcaggccca gggcgatgcg tccgcccgac agatcgagcg
      181 ggggaacggc ggggcgcgtc gattcggcgg gagccgcggt ttcggatgcg ccggcacttg
      241 caccgcgagg ggtttgtccg agaacgctca gcagttcctc ggccgccttg tcgattccgc
      301 tggctttcgg gctgcttgga gctgatgcgg gggcagaacc cacctgcatc tgaaacttcc
      361 agagactctg agtcgatgac atgctgctga tattcataca acctccttgt attgtaggac
      421 ctcagcgaag atcgagtcag aacactacct gctcagagag ttgaaatggg cgagcagggt
      481 ggcgcgttga tcaggattca ggaggtcacg gatccggaga acctcctgca gacgcgcatc
      541 gaacagcgac ctggacaatt ccttcacgcg tttctgttgc ttcgataatt cctccttgtc
      601 aggtgattga tgggcacgca gctgttgcag cccgcggcag gcggctgaca tgtgagattc
      661 gcgggtcgcg cgggcagtcg taaaggcgcg ccgataattg tccaggtgct ctttctgcat
      721 gggctcgagt ttgagctgtg tttcaaatgc gggagcatcg ggcgacagca gaagccattc
      781 gatgctgcga aggtcactcg acacgaattc cgtttcgtat tgcgtttctg gacgcgactg
      841 aactggtaca gtcgtcgacg gcgaggtggc aggtcgagac tgacgctgat agagagccca
      901 ggacatggca agcagtgcga tgatcagcaa ccgtagcaga tacgtgttgc gacgggtggc
      961 tgttcgacgg tgtgcgtcgg cgggggacgc gattgctgac tgatctgcgg gaacgggggt
     1021 gccggtctgt cccgaggctt tgcgggccag ttcttcctga attcggcgtt cgatctgagg
     1081 ccacaggtcg cgacgcggtc gtaccggttc cagcgactca gacggaacct gcagtgcctt
     1141 ctggatgaga tcccgatcgt gtcggcaccg ctcacacgtg gccagatgct cctcgagctg
     1201 gttccgggtg gtcgcgtcg
//
LOCUS       DAORHD010001598         1216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_165570_length_1216_cov_2.361757, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001598 DAORHD010000000
VERSION     DAORHD010001598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165570_length_1216_cov_2.361757"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..312)
                     /locus_tag="PKO06_23790"
     CDS             complement(112..312)
                     /locus_tag="PKO06_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72752.1"
                     /translation="MAAPGTLKRRMTEINKKTAKLADLLTKTTDSSGVTAANRIVGEG
                     ARRHWAQAGVDGIRKDRDEGVR"
     gene            878..>1216
                     /locus_tag="PKO06_23795"
     CDS             878..>1216
                     /locus_tag="PKO06_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV72753.1"
                     /translation="MRDSKLAPHGDREQQIRAFVSSACAGDIDAMRAQVAEGLDLNGR
                     DQFGDTLLELVISELEYCPEAPKYRVVQEMLRLGADPCGLSKDGSSPLFMAILNMDTE
                     MLRILLNGGAD"
BASE COUNT          254 a          346 c          352 g          264 t
ORIGIN      
        1 aattcgaccc gatcgatcag ttggtggatc gcgtcacgca gcctgtccgg gttgtcgttc
       61 agttcagtca gaatggttcg aagcagtttg cgtactgtgt ctgttgtgat ctcagcgcac
      121 gccttcatcg cggtcctttc tgattccatc gacgccagct tgagcccaat gccgtcgcgc
      181 tccttctcca acgattcgat ttgccgcagt aacgccgctt gaatccgtgg tcttggtcag
      241 caggtcagcc agtttagcgg tcttcttgtt gatttctgtc atccggcgct tcaaggtgcc
      301 tggggctgcc acggatctct cgcgctcctt cgacaaccgc tgagaatatt ctgcaatgac
      361 tgcggcgagg tcatcggctt ccagggcgcg cacccgcggt ggcggctttg ctgaccccta
      421 caccgggagt ttttcccgct cccgacagga gcttttccac tggcagcagt cgcttcggaa
      481 cgacagaatg tgaatctgga gcccgagtga ggagttgcca acatgctcga cagaaatgct
      541 gacaggccac agactgcagt atccgccaag caatccctga tgcctgcagc ggcgtgtacc
      601 cacgacaggg ggaaaacgtc cacgtcgatg acagttgcca tccaacacga agttcccgtg
      661 gcagagaaag gcctcgcggc tggccacggc tatcggcctc gatcgaacac ctgcccggtt
      721 ctgtagtcac aacatgtaca aagaccatga gcaggggaga tcggcaatgg cctgctgacg
      781 ctcacatgaa acgttgaacg ttggcccacc ctgtgggcac accatttcag gaatcggccg
      841 cacccaattg cctgcaacat cgagaaaagg acttcggatg agagacagca aactggcacc
      901 gcatggtgac cgcgaacagc agatcagggc cttcgtatcg agtgcgtgtg cgggcgatat
      961 cgacgcaatg cgtgcccagg ttgccgaggg cctggacctg aatggcaggg accagtttgg
     1021 cgatacgctg ctggaactgg tgatcagcga actcgagtac tgtcccgaag cgcccaagta
     1081 cagggtggtt caggaaatgt tgcggcttgg tgcagaccca tgtggtctca gcaaagatgg
     1141 ctcgagtcct cttttcatgg cgattctgaa tatggacacc gagatgttac gcatcttgtt
     1201 gaacggcggc gccgat
//
LOCUS       DAORHD010001599         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_165687_length_1215_cov_4.258621, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001599 DAORHD010000000
VERSION     DAORHD010001599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165687_length_1215_cov_4.258621"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKO06_23800"
     CDS             <1..466
                     /locus_tag="PKO06_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72754.1"
                     /translation="WEGSGETAHVARAYAEKFADAPQDRAGLLTDLYSSASIQGRVAV
                     LAPLFTAAVDAESAWARYHLAQSLQHWVRDLKSLAKAVGFKPTITTKVVGIGGLWSGW
                     PGFRARAVRVMEQTWPGEFTLTEPLLAPEWGPLVLARDTFGLSIADLQHFNR"
     gene            694..>1215
                     /locus_tag="PKO06_23805"
     CDS             694..>1215
                     /locus_tag="PKO06_23805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72755.1"
                     /translation="MNYRHSRLVLLLSVLVLWFGVVSPNIVVGADPSFGQPGWFSAKT
                     DQVLDRVADITQKKIRELAKFLDNRQNLDLELPIVFYDRQLRRVFVEYRGTVRFTGKW
                     PAKIVQDDYYFTSDGTLAWDCSLTNLRVTREAVTFALHGDLVISLDKLLFELASEITQ
                     AATTAAWYQGADQL"
BASE COUNT          212 a          359 c          391 g          253 t
ORIGIN      
        1 ctgggaaggc agcggcgaga cggcgcacgt ggcccgggcg tatgcggaga agttcgccga
       61 tgcgcctcaa gacagagcgg gtctgcttac cgacttgtac tcttctgcgt cgattcaggg
      121 gcgggtggcc gtgctggcgc cgctgtttac cgccgcggtc gacgcggaaa gcgcgtgggc
      181 acggtatcat ctggcgcagt cgctgcaaca ctgggtgcgc gacctcaagt cgctggcgaa
      241 ggcagtgggg ttcaagccga ccatcacgac caaggttgtc ggcatcggcg gcctctggag
      301 cggatggccg ggctttcgcg cgcgtgcggt tcgtgtgatg gaacaaacgt ggcccggcga
      361 gttcaccctg accgagcctt tgctggcccc tgaatggggg ccgctggttc tcgcccgcga
      421 cacgttcggc ctctcgatcg ccgacctgca gcatttcaac cgctgacgcc ggctctcagg
      481 ggtggcaacg gacaggtgca agcttgtccc tctttgccac actcattgtt cccgtctgcc
      541 tgtgcccggg cgtcgtcgaa tcgttgagtg atgccaaggc gaaaacccag gccggagaat
      601 cacatgaccc ggggtcactg ggcacgcgag ttgtcgtttt gccagaggta gattacacta
      661 gagtacagga aatcaaacag gcgaggcatg ctcatgaact accgtcactc gagattggtt
      721 cttctgcttt ccgttctggt gctctggttc ggggtcgtgt ccccgaacat cgtcgtcggt
      781 gccgacccgt cgtttgggca gcctggctgg ttctcggcca agaccgacca ggtgctcgat
      841 cgggttgccg atatcacgca gaagaagatc cgcgaactgg cgaagtttct cgacaatcgc
      901 cagaacctcg atctggaact tcctatcgtc ttttacgatc gccagttgcg ccgggtattc
      961 gtcgagtatc gtggaacagt gcggttcacg ggcaagtggc cggcgaagat tgtccaggat
     1021 gattattact tcacctctga tgggacactg gcctgggact gctcactgac caatctgcgc
     1081 gtgacgcgcg aggcggtgac cttcgccctg catggcgacc tggtgatctc gctcgacaag
     1141 ctgctcttcg agctcgcctc tgaaatcacg caggcggcga cgaccgcggc ctggtatcag
     1201 ggagccgacc agctg
//
LOCUS       DAORHD010001600         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_166230_length_1213_cov_2.588083, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001600 DAORHD010000000
VERSION     DAORHD010001600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166230_length_1213_cov_2.588083"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKO06_23810"
     CDS             complement(<1..188)
                     /locus_tag="PKO06_23810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72756.1"
                     /translation="MSSAKRDVIKAAPIIDGYSYPELFPLVKTFLQTGVSVLVRGHPG
                     VGKSTMARELARDLALPL"
     gene            complement(213..551)
                     /locus_tag="PKO06_23815"
     CDS             complement(213..551)
                     /locus_tag="PKO06_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72757.1"
                     /translation="MGRSRLSRPSLYREDYEERLRKAVRSVLPRYTLVEQHDPKQLGR
                     SWKVYGGKREYVVEILQGQRTSQGIWKRSCTCDDFTVHAPGPTNSCKHILAVILKHPD
                     LSYLLLDVFV"
     gene            complement(983..1213)
                     /locus_tag="PKO06_23820"
                     /pseudo
     CDS             complement(983..1213)
                     /locus_tag="PKO06_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
BASE COUNT          281 a          351 c          317 g          264 t
ORIGIN      
        1 atgagcggca gagccagatc gcgagccagt tcacgggcca tggtcgattt gccgacgccg
       61 gggtgcccgc gcaccagcac cgacacgccc gtctgcagga aggttttgac cagcggaaac
      121 agttccgggt aactgtaccc gtcgataatc ggtgccgctt tgatcacatc gcgttttgcc
      181 gagctcatat ctgcctccca tggttggtgt gttcagacaa agacatcgag cagaagatac
      241 gaaaggtcgg ggtgtttgag gatgacggcc agaatgtgct tgcaggagtt ggtcgggccg
      301 ggcgcgtgta cggtaaagtc gtcgcaagtg cagctgcgtt tccagatacc ttgtgacgtg
      361 cgctgaccct gcaagatctc gacgacatac tcacgtttgc cgccgtacac cttccacgat
      421 cgtccgagtt gtttcgggtc gtgttgctct acgagagtgt atctcggcag aacactccgc
      481 accgctttcc gcagtcgttc ctcgtaatct tcgcgataca gactgggccg tgacaatcga
      541 cttcgcccca tattcccctc cgctgttgaa caggttcact caccctgaac ccgccgaacc
      601 gccaacggtt cacagcaaac cgagaaaata tggggaatag agcacatcct cgccccgtgc
      661 cggcaagcat ctgcgttact tcggcgaccg catgaacatc aaacagctgt ttctcgtgac
      721 caccgaacct cgtctcgact ttatcgaaaa cggcgttcga acgatttcgc tcgcgcgctt
      781 tctcgccggc atggtttaat gatgacgaaa ccctgcgccc agacagccca aaccgaagat
      841 gtcgatggca atcagagcct ttctgagaac catgtccatt tcgggcagtt gatgacaaag
      901 caacgaaccc agagagaaaa gctcgttctt catcattgta ccgtccgaaa tccacttctc
      961 accagggaag aaatgggtcg ccgcctcggt tgaactgcgc tttcatcagt tcggaaacaa
     1021 tgtgggtttc gatgagactc ccccgtaaag ggtggatcga gacctgattg gcggtgcgaa
     1081 ttccgagaag ccatgacgcc agcccaacat catgaaaatt cagcttcggc atcttgcata
     1141 gtcacttgtt gtaattcgcg tgatgcggac gcagaagcaa agccacgcaa ctcgcttcca
     1201 gcaacgatat cca
//
LOCUS       DAORHD010001601         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_166373_length_1212_cov_4.389801, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001601 DAORHD010000000
VERSION     DAORHD010001601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166373_length_1212_cov_4.389801"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..427
                     /locus_tag="PKO06_23825"
     CDS             116..427
                     /locus_tag="PKO06_23825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72758.1"
                     /translation="MQTKLERHEQEYPKQASESQQDHGQGGNFRDEEVEQGFIGVAPP
                     LQQNQKRVADEADEAGKTTPFRNIVMQGYGGTSGFKLRSGHGSVELQLNQIEVRLQIT
                     G"
     gene            complement(362..>1212)
                     /locus_tag="PKO06_23830"
     CDS             complement(362..>1212)
                     /locus_tag="PKO06_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72759.1"
                     /translation="FWQAFMTFVGGDHRRAPQIIDSLFTDSTDKRLQSEARRLVSECR
                     RIERDEAAYYAFIARAKAGSAKGNTADREEALTWERAASGPRLLPETKKQAILLPSFP
                     IGHLGPDTEARVEPLRQLIASHSDSPHLLSEAGVLLAAAGALPDSLSCFEQILSNDEA
                     HPQARLAQARLLQATGAHERLQQELAKAVSLQPGHAGLQTAYALALMRWGDPWRGYLE
                     SRQALQLGSEAPELRLVRAEFFLALGRLPEARREVDLAKTCDDLPRDLQTHLNLIQLQ
                     LDASMP"
BASE COUNT          259 a          350 c          384 g          219 t
ORIGIN      
        1 gacttccaaa tcagatgtcg ttttatcctg ggccatgagc gtcgcgacaa acgtgagggt
       61 cagagccagg gctatcatca gacaacggtg aaaacgcatc gtacagactc ctttaatgca
      121 gaccaaactt gaacgccacg aacaagaata ccccaaacag gcatccgaga gccagcagga
      181 tcacggccag ggcggaaact ttcgtgacga agaagtcgaa cagggtttca tcggggtcgc
      241 gccgccactt cagcagaacc agaaacgagt cgccgatgaa gccgatgagg ctggcaagac
      301 aaccccattc aggaatatcg tcatgcaggg ctatggcgga acctccgggt ttaagcttcg
      361 ctcagggcat ggaagcgtcg agttgcagct gaatcagatt gaggtgcgtc tgcagatcac
      421 ggggtagatc gtcgcaggtt ttggccagat cgacttctcg acgtgcttca ggcagccggc
      481 ccaacgccag aaagaattcg gcccgcacca gacgcagttc aggtgcctct gagcccagct
      541 gcagagcctg acgcgattcg aggtagccgc gccaggggtc accccatcgc atcagagcca
      601 gggcataggc ggtttgcagg ccggcgtgac ctggttgcag cgacacggct ttcgccagtt
      661 cctgttgcag cctctcatgc gccccagtgg cctgcagcag gcgagcctga gccagacgag
      721 cctgcgggtg cgcttcgtcg ttgctcagaa tctgctcgaa acacgagaga ctgtcgggca
      781 acgcgccggc tgcggccagc agaactccgg cttcagacaa cagatgcggc gagtcgctgt
      841 gcgatgcgat cagctgacgc agaggttcga cccgggcttc ggtgtcgggg ccaaggtgtc
      901 cgatggggaa cgagggcagg agaatcgcct gcttttttgt ctcagggagc aggcgtgggc
      961 ccgaagcggc gcgttcccag gtcagtgctt cttcgcgatc ggcggtattc cctttggcgg
     1021 agcccgcctt ggcgcgtgcg ataaacgcat agtaggcggc ttcgtcacgc tcgatgcggc
     1081 ggcattccga aaccagtcgt cgggcctcac tctgcagacg cttgtcggtt gagtcggtga
     1141 acaggctgtc gataatctgc ggagcccgcc ggtgatcacc gccgacgaag gtcatgaacg
     1201 cctgccagaa cg
//
LOCUS       DAORHD010001602         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_167804_length_1206_cov_3.769765, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001602 DAORHD010000000
VERSION     DAORHD010001602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167804_length_1206_cov_3.769765"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PKO06_23835"
     CDS             complement(<1..565)
                     /locus_tag="PKO06_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNV72760.1"
                     /translation="MNTPSINLVAISEQYRGVTASLDQRVIVCAGTGCVANGALRVQA
                     ALEKAIREAGLPVVTQLKVEEKPKGTFVSHSGCQGFCQMGPLVTILPANILYCKVKAD
                     DVAEIVRETLQQGNVIERLLYHDPGTGKHCRGAEEITFYKRQTRTVLRECGLIDPDDI
                     REYISHGGYEGARKAYTELKPEAICEEV"
     gene            complement(562..1074)
                     /gene="nuoE"
                     /locus_tag="PKO06_23840"
     CDS             complement(562..1074)
                     /gene="nuoE"
                     /locus_tag="PKO06_23840"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNV72761.1"
                     /translation="MHAEATNLPSSVRQFEKVCEILDRYDRNATKLVPILQAVQEEYR
                     YLPEEVMAFVATSLRIPPAQVYGVATFYAHFALEPKGKYVVRLCDGTACHVKKSIPIL
                     EALRKRLKLDEGRTTTGDMLFTVETVSCLGACGLAPVLVINDDVHGQMTPESSLKLIE
                     EILKKEAETR"
BASE COUNT          239 a          345 c          358 g          264 t
ORIGIN      
        1 tcacttcttc gcagatcgct tcgggcttca gttcggtgta tgccttgcgt gccccctcat
       61 acccgccgtg cgagatgtac tcgcgaatat cgtccgggtc gatgagaccg cattcgcgaa
      121 gaacggtgcg ggtttgccgt ttataaaacg taatttcctc ggctccacgg cagtgctttc
      181 ccgttccggg gtcgtgatag agaaggcgct cgattacgtt cccctgctgc agcgtctcac
      241 gcacgatctc ggcgacgtca tccgccttga ccttgcagta gagaatgttc gccggcaaga
      301 tcgtcaccag cggacccatc tgacagaagc cctggcaacc gctgtgcgac acgaaggttc
      361 ccttcggttt ctcttcgact ttcagctgcg tgacgaccgg aagccccgct tcacgaatcg
      421 ctttctcgag cgccgcctgc acccggagag cgccgttggc cacgcagccg gttccggcgc
      481 agacaatgac ccgctggtcc agtgacgccg tgacgccacg atactgttcg gaaatcgcga
      541 caaggttgat ggacggcgta ttcatcgggt ttccgcctcc tttttcagaa tctcttcgat
      601 cagtttgagt gacgattcag gggtcatttg tccatgcaca tcatcgttga tcaccagcac
      661 tggagcgagt ccgcaggctc cgagacagga gaccgtctcg acggtgaaca gcatgtcacc
      721 ggtcgtggtg cgcccctcgt cgagtttcag gcgtttgcgc agcgcttcga gaatcggaat
      781 cgacttcttg acgtgacagg cggtaccatc acagagccgc acgacgtatt ttcccttggg
      841 ctcgagcgca aagtgtgcgt agaacgtggc cacgccgtag acctgagcgg gcggaatgcg
      901 cagggaggtc gcgacaaagg ccatgacctc ttcgggcagg tagcgatatt cctcctgaac
      961 cgcctgcagg atgggtacga gcttcgtggc gttgcgatcg taccggtcga gaatttcgca
     1021 gaccttctcg aactgacgca cagaggatgg aagattggtt gcttcagcat gcatatgggg
     1081 gaactcctcc ttggaaaact cgaacgtggt gtgaaattgt gaggtgaaat gaggacaaaa
     1141 gggacctcag ccggagcgga tcaccgcggt gggcgctctg cgtccgtcag atcgatcagg
     1201 aatagg
//
LOCUS       DAORHD010001603         1205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_168077_length_1205_cov_2.916522, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001603 DAORHD010000000
VERSION     DAORHD010001603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168077_length_1205_cov_2.916522"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKO06_23845"
     CDS             <1..384
                     /locus_tag="PKO06_23845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72762.1"
                     /translation="TRNLKFDQLNDVSFQTRFTEPVTVEWRGYIARSFQRYGRHFLLM
                     IKELNQDELDNMGRDRNDYRLNPNFNNQPLFLLIADKGKFPPFMKQGSFITFTGVTDW
                     KKYDILNELGQTVKLPAFEFLSAYP"
     gene            639..1112
                     /locus_tag="PKO06_23850"
     CDS             639..1112
                     /locus_tag="PKO06_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV72763.1"
                     /translation="MSTHFHGSTGENRALATFIKLMRAADTVLAVSRRIYAEAGLTDS
                     QFGVLEALLHLGPLTPGELGKKILKSAGNLTLVIDNLVKHDLIERRESREDRRQRPVS
                     LTAKGRELIQNLFPRHVSQIVHVMSALAEHEQDQLSDLLRKLGKHSSYTKIHLTT"
BASE COUNT          286 a          349 c          292 g          278 t
ORIGIN      
        1 acccgcaatc tgaagttcga tcagctgaac gacgtcagct tccagaccag attcacggaa
       61 ccggtgaccg tcgagtggcg tggctacatc gcccgctcct ttcagaggta tgggcgccat
      121 ttcctgctca tgatcaaaga actgaatcag gacgaactgg acaacatggg cagagatcga
      181 aacgactacc gcctcaatcc caacttcaac aatcagccat tgttcctgct gatcgccgac
      241 aaaggcaagt ttccgccctt catgaagcag gggagcttta tcaccttcac cggtgtcact
      301 gactggaaaa agtatgacat tctgaatgaa ctgggacaaa cggtgaaact gcccgcgttc
      361 gagttcctct ccgcgtatcc ctgaactccc gcactctgtc tatcgtgggc ctcggcgatt
      421 gacgaggccc attctcttca tcggcctgtt cgcctgccgc agagtccatt ctccgttcca
      481 cagccattgt cggtggacaa agcacgcccg atcgggtggc attgattccc tcgcgctcgc
      541 ggcctgtcag aatacgccac gaacaagaat tagataaatc taacattttt cttgacatta
      601 ttttaatatc atatattttg atattaaagg atgttgatat gagcactcac ttccatggtt
      661 cgaccggcga gaatcgtgca cttgccacct tcatcaagct gatgcgggct gctgacaccg
      721 ttcttgccgt cagtcggcgc atctatgccg aggcgggcct gaccgacagc caattcggcg
      781 ttcttgaggc attactgcac cttggcccgc tcactcccgg cgagctcggc aagaagatct
      841 tgaaatccgc cggcaatctg acgctcgtca ttgacaacct ggtgaagcat gatctgatcg
      901 aacgtcgaga atctcgtgaa gatcggcggc agcgaccggt ttccctgaca gcgaagggtc
      961 gagaactgat tcaaaacctg tttccccggc atgtctctca gatcgtacac gtgatgtcgg
     1021 ctctcgcaga gcacgagcag gaccagttgt ctgatctgct gcgcaagctg gggaagcaca
     1081 gctcgtacac gaagatccat ctcactacct aaccatatcg gaggaactct atgaaaaccc
     1141 tgttgcacat cattgccacc ccgcgcgggt cggcttcttc aacgctcaag atcagtgaag
     1201 aaatt
//
LOCUS       DAORHD010001604         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_168286_length_1204_cov_3.053090, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001604 DAORHD010000000
VERSION     DAORHD010001604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168286_length_1204_cov_3.053090"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..>1204
                     /locus_tag="PKO06_23855"
     CDS             203..>1204
                     /locus_tag="PKO06_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72764.1"
                     /translation="MTGLKSSPQASDAAMISSYSSAMKAFVDELMRPIRTTEVPTSLA
                     LVGPGKFFIGWARFVGNPEDALGDQMMNTVMSLICRDHLVRANPQLREEDETDSPTSP
                     GLTQDTVKAGMMKEEFVKMLRDIAGPDTFLHIFKFPQRVTEIGVGSGRMFFCFLPVRW
                     QGVVRYLMYPNWTESIVDLPFITETLSASSSGNIYPGIFACLKNPETQRISQRFDSIP
                     AGLAAHPDLSALVKAVRVSNTPLREYNAKSDRILEAIPARHINLFILAGERSAEHLRD
                     RASHHWNLFLAVLGFILLLSIGVAFSLAEYFLAPLKRVLLGIAEIDAGNYRVHLDGTR
                     "
BASE COUNT          274 a          345 c          319 g          266 t
ORIGIN      
        1 ggctggtgcc tttgggttgg ggaaacgctt catagctgaa aacgaacagc gagaacgcgg
       61 gcgcatagct acccacctga gtcaggatct tattcgtctt gatgacgctt cgcgcctgtt
      121 tcaggggaac ctgattcagc gaatgaaaaa ccttggttcc aacgcgcaac tcgtcaacct
      181 cgtgtatcag gaagagatcc agatgaccgg cttgaagtct tcacctcagg catctgatgc
      241 ggcgatgatc tcgtcatatt cttccgccat gaaagcattt gtcgacgaac tcatgagacc
      301 catacgaacc acagaagtgc caacctcgct ggctctggtc ggcccaggta aattcttcat
      361 cggatgggca cgattcgtgg gcaatccgga agacgctctc ggcgaccaga tgatgaatac
      421 cgttatgagt ctcatctgcc gagaccacct cgtacgcgcg aacccgcaac ttcgagaaga
      481 ggacgagacg gattcaccca cgtcgcctgg cctgacgcag gatacggtca aagcgggcat
      541 gatgaaagaa gagttcgtca aaatgctgcg cgacattgcc ggcccagaca ccttcctgca
      601 catcttcaag tttccgcagc gggtcaccga aatcggcgtg ggaagcggac gcatgttttt
      661 ttgcttcctc ccggtcaggt ggcagggggt cgttcggtat ctgatgtatc caaactggac
      721 ggaaagcatc gtcgatctgc ccttcatcac tgaaacgttg tcagcgagca gttcaggcaa
      781 catctatccc ggtatcttcg cctgtctgaa aaacccagaa acccagagaa tctcacagcg
      841 tttcgacagc atcccagccg gcctggcggc acacccagat ctttccgcgc tcgtgaaggc
      901 cgtgcgcgtc tcgaacactc cgttgagaga gtacaatgcc aagtctgacc gcatactcga
      961 agccattccc gcacgtcata tcaatctgtt tattctggct ggagagcgct ctgccgagca
     1021 tctcagagac cgggcatcac atcattggaa tctttttctg gcagtgctcg gcttcatcct
     1081 gcttctctcg ataggagtcg ccttcagtct ggccgagtac ttcctggcac cgctgaagcg
     1141 ggtgttgctc ggcatcgcgg aaattgacgc agggaattac cgagtgcacc tcgatggcac
     1201 gcgc
//
LOCUS       DAORHD010001605         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_168315_length_1204_cov_2.701480, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001605 DAORHD010000000
VERSION     DAORHD010001605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168315_length_1204_cov_2.701480"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..466
                     /locus_tag="PKO06_23860"
     CDS             119..466
                     /locus_tag="PKO06_23860"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV72765.1"
                     /translation="MGCPDRRSVDRPGALTYSKKVMLTVGSGITALDPFSAMITVFSH
                     SVSLMVFTALHVPVSSSQAIVGSVLGIGWLGGGRTISYRTLWFILIGWLLTPFSGALV
                     AYLMKVGFDRLIG"
     gene            494..1147
                     /locus_tag="PKO06_23865"
     CDS             494..1147
                     /locus_tag="PKO06_23865"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HNV72766.1"
                     /translation="MFFNTSKEDKVIALMGKHLAEVKECLALFQSFMVLYCKEASEQE
                     LKDTSYKVHVKEHDADLKVKEIQRELIEGAFLPFYRENFLNIPELIDRIPGLAVKICK
                     EITLQPLPIPGELKEFLLESTAAVLETFDEFLHLFEYVPNDLEKVIEVSQTVSKCEQK
                     VDTLEWKSKSYLFKTNTTMDRLDKFFFKDLITLIADMADKIENVSDYIQLTMIKMKV"
BASE COUNT          284 a          324 c          307 g          289 t
ORIGIN      
        1 aactgggtgc gaacaatgtc gtggtcacca cgggccccta ctatcaggca gggctcttcg
       61 gcccgcctct gcggggagaa gctttcgatc aggtcagctt ctggaaccac cccgccttct
      121 gggctgccct gatcggcggt ctgtcgatcg ccctggggcg ttaacgtaca gcaagaaggt
      181 catgctcacg gtcggtagcg gcatcaccgc tctcgatccg ttctctgcca tgatcacggt
      241 cttctcgcat tccgtctcat tgatggtgtt taccgcgctg catgttccgg tgtcatcgtc
      301 tcaggccatc gtcggatctg tactcggcat cggctggctg gggggcggac gcaccatcag
      361 ttatcgcaca ctctggttca ttctcatcgg ctggctgctg actccgttct ccggagctct
      421 ggtcgcctat ctcatgaagg tcggcttcga ccgtctgatc ggatgaaacc caatttctga
      481 ggggaggaaa cacatgttct tcaacacttc caaagaggac aaagtcattg cgttgatggg
      541 gaaacatctc gccgaagtca aggaatgcct cgctctgttt cagtcgttca tggttctcta
      601 ttgtaaagaa gcctcagagc aggaattgaa ggatacctcc tacaaggttc atgtcaaaga
      661 gcacgatgct gacctgaaag tgaaagagat acagcgcgaa ctgatcgagg gagcctttct
      721 gcccttctat cgcgaaaact ttctgaacat ccccgaattg atcgatcgca ttcccggtct
      781 ggcagtgaag atctgcaagg aaatcaccct tcagccgttg ccaattcccg gtgaactgaa
      841 ggagtttctg ctcgaatcga ctgccgccgt gctcgaaacg tttgacgagt ttctgcacct
      901 cttcgagtat gtgcccaatg atctcgagaa agtgattgaa gtttcgcaga cagtctcgaa
      961 gtgcgaacag aaggtcgaca cactcgaatg gaagtcgaaa agttatctct tcaagaccaa
     1021 cacgaccatg gacagactgg acaaattctt cttcaaggat ctgatcaccc tgatcgccga
     1081 catggcggac aagatcgaaa acgtctccga ctacattcag ctgaccatga tcaagatgaa
     1141 ggtctgaagc tgatatcaag ccattacatc tcggacgttc aaaaacgtcg agtcagtagt
     1201 agta
//
LOCUS       DAORHD010001606         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_168560_length_1203_cov_2.776132, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001606 DAORHD010000000
VERSION     DAORHD010001606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168560_length_1203_cov_2.776132"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PKO06_23870"
     CDS             complement(<1..485)
                     /locus_tag="PKO06_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72767.1"
                     /translation="MKQIKGLVVLALLMAVAIGCEASALLVTCEVGGAKLERYNETTH
                     RWDRIGGMPYLITVQRYEYVMIRVTAAGFKEFEKGYEVRTDGTTRAHVVLEPENDNPT
                     STSLFSIGGQVTRKSGMSILRGQYTMMARNMTTYRSGTGQMAHDENPIEDDGWYTCTW
                     A"
     gene            618..>1203
                     /locus_tag="PKO06_23875"
     CDS             618..>1203
                     /locus_tag="PKO06_23875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV72768.1"
                     /translation="MHNKLINAGLGSIMGALVGDAAGAYLEFLGRAPSDQEIDRAMSL
                     PGGGTWNVAHGQITDDGELTLCLAQALLESRGFDIETVARWYAKWFASPPFDIGNTTR
                     SSIGCVMHMDVETLTRNKQFAESMTRAAAAKCRGSMANGSLMRISPLGAWGHSLPNAE
                     LARLAGLDSSLSHPNETCCHAVGAYCIAIAELVRH"
BASE COUNT          227 a          379 c          319 g          278 t
ORIGIN      
        1 tcggcccagg tgcaggtgta ccagccgtcg tcctcgatcg ggttctcgtc gtgcgccatc
       61 tggcccgtgc cacttcgata ggtggtcatg ttccgcgcca tcatggtgta ctgcccgcgc
      121 agaatcgaca tgcccgattt tcgtgtcacc tggccgccga tgctgaacag cgacgtcgag
      181 gtcgggttgt cattctcggg ttcgagcacc acatgtgctc tcgtcgttcc atcagttcgg
      241 acttcatacc ccttttcgaa ctccttgaac cctgcggcgg tcacgcgaat catcacatat
      301 tcgtatctct gaaccgtgat cagatagggc atgccaccga ttcgatccca gcggtgcgtg
      361 gtttcgttat accgttcaag cttcgcgccg ccaacctcgc aggtcaccag caacgcgctc
      421 gcctcgcacc ctatcgccac cgccatcaac aacgccagaa ccacgagacc tttgatctgc
      481 ttcatacgtt cctcctgtga cgattttgcc gttttcgttg ccgaccttac atattccgga
      541 aacgatggtt gacttgcttt ttttccgccc cacctgctat ttctggttca tcacacgatc
      601 ctgacggagg cggacccatg cacaacaagc tgatcaacgc cggactcgga agcatcatgg
      661 gcgccctggt tggcgatgcc gcgggcgcat acctcgagtt tctcggtcgg gctccatccg
      721 atcaagaaat cgatcgcgcc atgagtctgc cgggtggagg aacgtggaat gtcgctcacg
      781 ggcagatcac cgacgacggg gaactgacgc tgtgtctggc tcaggccctt ctggaatctc
      841 gcggtttcga catcgagacg gtcgctcgct ggtatgccaa atggtttgcc tcgcctccct
      901 tcgacatcgg gaataccaca cggtcctcta ttggttgtgt catgcacatg gacgtcgaga
      961 ccctcaccag aaacaaacaa tttgcggaat cgatgacccg ggccgcggcg gcaaaatgtc
     1021 gagggtcgat ggctaacggc agtctgatgc gcatttcccc gcttggggcc tggggtcact
     1081 cactcccgaa tgcagaactc gcaagactgg cgggccttga ttcatccctt tctcacccga
     1141 acgaaacctg ttgtcacgca gtcggagctt actgcatcgc catcgcggaa ctggtcaggc
     1201 acg
//
LOCUS       DAORHD010001607         1201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_168992_length_1201_cov_3.388307, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001607 DAORHD010000000
VERSION     DAORHD010001607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168992_length_1201_cov_3.388307"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1201
                     /locus_tag="PKO06_23880"
     CDS             <1..>1201
                     /locus_tag="PKO06_23880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNV72769.1"
                     /translation="TRARGSSAAALTELMQLQPQRACILRDDAEQMVAVDQVRVGDLL
                     IVRPGEKVPVDGLIREGRSTIDESMITGESMPVTREFGEAVIGGTINRDGLLKVEALK
                     TGRDTVLAQIVQMVRQAQAAKAPIQQTTDQIGRYFVPTIVFLAICTFFGWKLVARIDT
                     MGALLNAIAVLVIACPCALGLATPTAITVGMTRGAELGILFRSAEALERAGHADIVVL
                     DKTGTITRGKPEVTDIVPVAGFTEKDLLMLAASAELGSGHPIGLAVIKAAEERGLKPL
                     IPEQSQVLSGTGVEAGIGSRKVFVGNARLISARQINLSKLQDEMTRLQSQGKTTMIVA
                     ETTTGSADVPVVRGLIAVADTVKAGAKEAVAALRTMGLSPIMVTGDHQKTAASMAEQV
                     GITRVVAE"
BASE COUNT          252 a          354 c          373 g          222 t
ORIGIN      
        1 aacgcgagcc cgtggctcat cagccgcggc tctcaccgaa ctgatgcagc tgcaaccgca
       61 acgggcctgc atcttgagag acgacgccga gcagatggtc gccgtcgacc aggttcgggt
      121 cggagacctg ctgatcgtga ggcccggcga gaaggttcca gtcgatggac tgatacgcga
      181 agggcgttcg accattgatg aatcgatgat caccggtgag tcgatgcccg tcacacgaga
      241 attcggtgag gctgtcatcg gcggcaccat caaccgcgac ggacttctga aagtcgaagc
      301 cctgaaaacc ggcagagaca cggttctggc gcagattgtc cagatggttc gacaggccca
      361 ggccgccaag gctccgattc agcagaccac cgatcagatc ggtcgttact tcgtgccgac
      421 catcgtcttt cttgccattt gtaccttctt cggctggaag cttgtcgccc ggatcgacac
      481 catgggagcc ctgctcaatg ccatcgcggt actggtcatt gcctgcccct gcgctctcgg
      541 cctggcaaca ccgacggcaa ttacggtcgg catgacccgg ggtgccgaac tgggcattct
      601 ctttcgctcg gccgaagcgc tcgagcgggc cggtcacgcc gacatcgtgg ttctcgacaa
      661 gaccggcacc atcacgcgcg gcaagcccga agtgaccgac atcgtccccg ttgccgggtt
      721 cacggaaaaa gacctgctga tgcttgcggc atctgccgag cttgggtcgg gtcacccgat
      781 tggtctggcg gtgatcaagg cggcagaaga acgcggcctg aaacctctga ttccggaaca
      841 gtctcaagtg ctttccggta cgggggtaga agccggtatc gggagtagaa aagtcttcgt
      901 cggcaacgcg cggctgataa gcgcgcgaca gattaacctg tccaagctgc aagatgagat
      961 gacccgcctg caaagccagg gtaagacgac catgattgtg gccgagacaa cgactggatc
     1021 ggctgatgta ccagttgttc gcggcctgat cgctgttgct gacacagtca aggcaggtgc
     1081 aaaagaagct gttgcggcat tgcgcaccat gggtctcagc ccgatcatgg tgacgggcga
     1141 ccatcaaaag acggccgcca gtatggccga acaggtgggc atcacccgcg tggtggccga
     1201 g
//
LOCUS       DAORHD010001608         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_170229_length_1196_cov_3.339176, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001608 DAORHD010000000
VERSION     DAORHD010001608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170229_length_1196_cov_3.339176"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1196)
                     /locus_tag="PKO06_23885"
     CDS             complement(<1..>1196)
                     /locus_tag="PKO06_23885"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV72770.1"
                     /translation="SDSTLKQLRQIVPLNLLSSNRYALLFPLIRLHNRVADYMIVFLW
                     NFRTSVRLFLDAALLPANRRESGLRLVIHENATGSFFSSGFKLSANLKQFLQRVNSRD
                     HLFSSVLTLPEGRYLAAGLPAQNLSGFTLCILFPYDRIRGQLSQTRFWLNFLLSVNLL
                     LSLVLSVLIARRFLGPVARLSQAIMLTQRNRSQERVEELAADELGQLAQAFNSALDNF
                     EELNLGRIVQEQLFPVESLDNGGFRLFGRSVAMTDLGGDFFDLIRLDDRRTAIIFGDV
                     AGHGVPAALVMAMAKAFFHERPAETFLATKLLGEFSALLYSLKSGTSRRMMTMMIAIL
                     DSGSGRVDIVNAGQTFPLLVNGDAGTARFLEIPGAPAGTLKKLRLGEATTTLEPGDTL
                     VLYTDG"
BASE COUNT          280 a          343 c          363 g          210 t
ORIGIN      
        1 cccatcggta tacagcacca gggtgtcgcc cggttccaga gtcgtggtgg cctctcccaa
       61 tcgcagtttc ttcagcgtgc ctgccggagc gccgggaatc tcgagaaacc gggccgttcc
      121 cgcatctccg ttcaccagca gcggaaaggt ctggccggcg ttcacgatgt cgacgcgacc
      181 gctcccgctg tccagaatgg cgatcatcat ggtcatcatc cgccgcgatg tgcccgactt
      241 cagagaatac agcaacgcgc tgaactcccc cagcagtttg gttgcgagaa aagtctccgc
      301 ggggcgctcg tgaaaaaagg ctttggccat ggccatgacc agcgcggccg gaacaccgtg
      361 tccggcgaca tctccgaaga tgatggcggt tcgccggtcg tccaggcgaa tcaaatcgaa
      421 gaaatctccg cccaggtcgg tcatcgcgac actgcgtccg aaaagacgaa aacctccgtt
      481 gtccagactc tcaaccggaa aaagctgctc ctgaacaata cgaccgaggt tgagttcctc
      541 gaagttgtcg agagcggaat tgaacgcctg ggccagctgt cccagttcgt cggcggccag
      601 ctcttcgacc cgttcctggc ttcgattgcg ttgtgtcaac atgatcgcct gggacaatcg
      661 cgccaccggg ccgaggaagc ggcgggcaat cagaacgctc agaaccagcg acagcaggag
      721 attgaccgac aggagaaaat tcagccagaa tcgggtctgg ctgagctgac cacggatccg
      781 atcataggga aagaggatgc acagcgtgaa tccggagaga ttctgggctg gcagaccggc
      841 ggccagatag cgaccctccg gcagagtgag gacgctcgaa aagagatggt ctcgcgagtt
      901 aacacgctgc agaaactgtt tcagattcgc gctgagcttg aatcctgacg aaaagaacga
      961 gcccgtggcg ttttcatgaa tcaccagccg cagcccgctc tcgcgtcggt tggccggcag
     1021 cagcgcggca tccagaaaaa gccggacgct ggtgcgaaag ttccagagaa agacgatcat
     1081 gtagtccgcc acccggttgt gaaggcgtat caacgggaac agaagggcat accggttcga
     1141 actgagaagg ttcaggggaa cgatctggcg aagttgcttc agggtcgaat cagagg
//
LOCUS       DAORHD010001609         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_170275_length_1196_cov_2.729185, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001609 DAORHD010000000
VERSION     DAORHD010001609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170275_length_1196_cov_2.729185"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="PKO06_23890"
     CDS             complement(<1..605)
                     /locus_tag="PKO06_23890"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938903.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III"
                     /protein_id="HNV72771.1"
                     /translation="MKFSRVFLEAIGYELPKNVITSTWIEEKLAPLYRKLFMQPGQLE
                     ALTGIRERRWWDPGHTNAEGATKAVQKALADSQVPVEDIGAVVYGGVCRDHFEPATAC
                     QVAAELGVQAGTLIFDISNACLGVMNGIIDIANRIELGQIKAGVVVACETAREITGIM
                     MEQMMTENTMDFFKNALATLTGGSGAVGIVLTDGSYAPDKP"
     gene            complement(634..>1196)
                     /locus_tag="PKO06_23895"
     CDS             complement(634..>1196)
                     /locus_tag="PKO06_23895"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HNV72772.1"
                     /translation="PYQPSEAEIRRAVPLGLEDTVPLTPEDTRSLTLLGAQMPARSWR
                     MNETIDLFIPDGGPHGLGFIRGRKPVDPTEWFFKAHFYQDPVIPGSMGLEAFLQLLRK
                     VALYRWGTDCRGPEWQFEPIAVGQPHVWSYRGQVIPTNKMVEVDAVIEEIDDADKKIR
                     ASGYLKVDGLVIYKVDNFAIRLARGT"
BASE COUNT          249 a          350 c          341 g          256 t
ORIGIN      
        1 tggggtttgt cgggggcata cgaaccgtcg gtgagaacga tacctacggc gcctgagcca
       61 ccggtgagcg tcgccagggc attcttgaag aagtccatgg tgttctcggt catcatctgt
      121 tccatcatga ttcctgtgat ttcccgtgcg gtctcacagg cgaccaccac accggccttg
      181 atctggccga gttcgatgcg gttcgcgatg tcgatgatgc cattcatgac gcccagacag
      241 gcattggata tatcaaaaat cagagtgcca gcttgcactc cgagttcggc ggccacctga
      301 caggcggtgg ccggttcaaa gtggtcccgg cagacgccgc catagacgac ggcaccgata
      361 tcctcgacag ggacctggga atcggcaagg gccttctgaa ctgctttggt ggccccttct
      421 gcgttagtat gacccggatc ccaccagcgg cgctcacgaa ttccggtgag ggcttcgagt
      481 tgtccgggtt gcatgaacag cttgcggtac agcggcgcga gtttttcttc gatccaggtg
      541 gaggtgatga cgtttttcgg cagttcatag ccgattgctt caaggaacac ccgggaaaac
      601 ttcattgcag gacctttcga tgaatataag gaattacgtg ccgcgcgcca accgaatggc
      661 gaaattatct accttatata tcaccaatcc gtcaaccttt aaataccccg aggcacggat
      721 ttttttgtct gcatcatcga tttcttcgat cacggcatcg acttcgacca tcttgttcgt
      781 cggaatcacc tgcccgcgat aggaccagac atgcggctga ccaaccgcga tcggctcgaa
      841 ctgccattcc ggcccacgac aatcggtgcc ccaccggtac agcgcgactt tgcgcagcag
      901 ctgcagaaat gcctcgagcc ccattgagcc gggaatgacg ggatcctggt agaaatgggc
      961 cttgaagaac cattctgtcg gatcaaccgg tttgcggccg cgaatgaaac caagcccgtg
     1021 ggggccgcca tccggaatga acagatcgat ggtctcgttc atgcgccagg accgggccgg
     1081 catctgcgca cccagcaacg tgagactgcg cgtgtcttcc ggagtcagag gcacggtgtc
     1141 ttcgagcccc agcggcaccg cccggcgaat ctcggcctcg ctcggctgat acggag
//
LOCUS       DAORHD010001610         1195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_170456_length_1195_cov_4.267544, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001610 DAORHD010000000
VERSION     DAORHD010001610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170456_length_1195_cov_4.267544"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..870
                     /locus_tag="PKO06_23900"
     CDS             220..870
                     /locus_tag="PKO06_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72773.1"
                     /translation="MQRTSGLPMRALIVVLGLLLGMGTPAQAQIASEQVFDLQWVDLY
                     AELLPERNEAQVIAHLYLDSNGVDPVVFRLEGNFLTWKISSYNNQQPNLAFRRDGFLF
                     WLYNLASGPAEVVFEYQVRRQGWETPDGAVIATNKLELGPGAFWYPRNVASDSHQAIL
                     NLVTPPDYAVNAPASLTRDLPHNFKRLRTYILRTPTQAGLTLSSSASRAAAWGKVP"
     gene            872..1063
                     /locus_tag="PKO06_23905"
     CDS             872..1063
                     /locus_tag="PKO06_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72774.1"
                     /translation="MRRQQRPATPEEQRRGCLVMVVFIGAAIILNGVMLALPITLPEW
                     VWQVVFFLMVTFGIWLFAA"
BASE COUNT          222 a          331 c          350 g          292 t
ORIGIN      
        1 cttttcgctg aaattgctca gcagggacac ccgactcaac caggccagca gttctgtttc
       61 ggacatgtgt tcctcgaatt gaaagtgtct tagtatagca tagaccgcgt cggccgttca
      121 aaggggagga ttcgcttgac aaacgtcttc tgcggaatac tttattgcct caatgacgtt
      181 gtccgcgatg gttggtcacg ggatcaatgg agtaacacga tgcaacgaac gagtgggctg
      241 ccgatgcggg cactgatcgt ggtgctgggg ttgctgctgg gcatgggaac gccggcgcag
      301 gctcagattg cgagtgaaca ggtttttgac ctgcagtggg tcgatctgta tgccgaactt
      361 cttcctgagc ggaacgaagc gcaggtcatt gcgcatctgt atctcgacag caatggcgtg
      421 gatccggtcg tcttcaggct cgagggcaac tttctcacct ggaaaatttc gagttacaac
      481 aatcagcagc cgaacctggc ttttcggcgc gatggcttcc tgttctggtt gtacaatctg
      541 gcatccggtc cggcggaagt ggtcttcgag tatcaggtgc gtcgtcaggg ctgggaaacc
      601 ccggatggcg cagtgatcgc cacgaacaaa ctcgagctcg ggccaggggc cttctggtac
      661 ccgagaaatg tcgcttcaga ttcccatcag gcaattctca atcttgtgac cccacctgac
      721 tatgcggtca acgcaccggc ttcgctgact cgcgatctgc cgcacaattt caaacgcctg
      781 cgcacctaca ttctgcgcac accgacccag gctggcctga ccctgtcgag ttcggcttcc
      841 cgcgcggctg cgtggggtaa ggtgccgtag catgcgtcgc cagcaacgcc cggccactcc
      901 cgaggaacag cgtcgtggct gtctggtcat ggtggttttc atcggtgccg cgataattct
      961 gaacggggtc atgctggctt tgccgatcac tcttcccgag tgggtctggc aggtcgtgtt
     1021 ctttctgatg gttacattcg gcatctggtt gtttgccgcc tgaaaatgtg actgctttac
     1081 ccaccggagc gaacggggta gaatgtgccg acattcttgc tcgccgggag gttcacatgc
     1141 atcgtccgtt catcgctctc ttcgctctgc tgcttctgct ggggccgatc acgac
//
LOCUS       DAORHD010001611         1194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_170832_length_1194_cov_2.387182, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001611 DAORHD010000000
VERSION     DAORHD010001611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170832_length_1194_cov_2.387182"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1194
                     /locus_tag="PKO06_23910"
     CDS             <1..>1194
                     /locus_tag="PKO06_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72775.1"
                     /translation="HDTRLLGLYLTSFSCGPDSFILHFFNHEMNRTGRPYLELELDEH
                     SAGAGVETRMLAFIDVIKNQQEVHTLPTGVAIQTTKAVAPLAGRTIFLPKMSEGSHCL
                     AAAFNGVGAKAKVMETYTREGMEFGKTHTSGKECFPCTVTTGDMFHLIETLKNDGADV
                     ANEIAFFMPETEGPCRFGQYNRLHRILLDKLGLGRIPILSPSSEDSYRCDGLFTDEQA
                     SFFRRLGWEAIVYADLIEKSLWRVRPYEKVPGTTDRVFHEAMKNGVEAIQRGGGLRLL
                     KVARQAAQAFEQIERVNGKAKPLVGIVGEIYVRTHKESNQELVRTLEEMGCETFTSSI
                     SEWIEYTTFSSIEDANDRIRKYGGFGNYKDLARNYVTGRYQRMVSWMLGRPFAGLLKN
                     RFDHET"
BASE COUNT          255 a          360 c          349 g          230 t
ORIGIN      
        1 ccacgacacg aggctgttag gcctgtatct gacctcgttc tcgtgcggcc ctgattcgtt
       61 cattctgcac ttcttcaacc acgagatgaa tcgtaccggt cgcccgtatc tcgaactcga
      121 gctggatgag cattctgctg gagcaggagt cgagacccgc atgctggcgt tcatcgacgt
      181 catcaagaac cagcaggaag tccacaccct gccgaccggg gtggccattc agacaacgaa
      241 ggccgttgcg ccgctcgccg ggcgcacgat tttcctgccg aaaatgtccg agggctcaca
      301 ctgtctcgcg gccgccttca acggggttgg cgctaaagcg aaagtcatgg aaacctatac
      361 ccgcgagggc atggagttcg gcaagacgca cacttcgggc aaggaatgct ttccctgcac
      421 ggtcacgacg ggcgacatgt ttcacctgat cgaaactctc aagaatgacg gcgcagacgt
      481 agccaacgag attgccttct tcatgcccga gaccgaaggt ccgtgtcgat tcggccagta
      541 caatcgcctg catcgcattc tgctcgacaa gctcggcctc gggcgcattc cgattctgtc
      601 gccgagctcc gaagacagct atcgttgtga cgggttgttc accgacgagc aggcgtcgtt
      661 cttccggcga ctgggctggg aagccatcgt ctacgccgac ctgatcgaga agtcgctgtg
      721 gcgcgtgcgg ccgtatgaga aggtgcccgg caccaccgac cgcgtcttcc acgaggcgat
      781 gaagaacggg gtcgaggcga ttcagcgcgg cggcgggctg cgtctgttga aagtcgctcg
      841 tcaagcagct caggcgttcg agcagatcga gcgcgtcaac ggcaaggcca aacccctcgt
      901 cggtatcgtc ggcgagattt acgttcgcac tcacaaagag agcaaccagg aactggtacg
      961 cacgcttgag gagatgggct gcgaaacttt caccagctcg atttcggaat ggatcgaata
     1021 cacgacgttc agcagcatcg aggatgcgaa cgaccgcatt cgcaagtacg gcgggttcgg
     1081 caactacaaa gatctcgccc gcaactatgt gaccggtcga tatcagcgca tggtctcctg
     1141 gatgctcggc cgcccattcg ccggcttgct caagaaccgc ttcgaccacg aaac
//
LOCUS       DAORHD010001612         1188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_172232_length_1188_cov_3.933804, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001612 DAORHD010000000
VERSION     DAORHD010001612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172232_length_1188_cov_3.933804"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKO06_23915"
     CDS             complement(<1..451)
                     /locus_tag="PKO06_23915"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV72776.1"
                     /translation="MILFLSDLHGWYGLVNEQIRHAEARFHEKIAAVIELGDFGLFGP
                     ALHEHFLMRNERFLRPFYFVEGNHEDFTHFETLTRVYHSHLTHIPRGSVQEIGGKRVL
                     GFGGASYMDPRITPHGSVIRDEDIDRCLALPDTTVDIIVSHDCPKGLG"
     gene            complement(465..>1188)
                     /locus_tag="PKO06_23920"
     CDS             complement(465..>1188)
                     /locus_tag="PKO06_23920"
                     /inference="COORDINATES: protein motif:HMM:NF021824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HNV72777.1"
                     /translation="AWFLSLGGMVCEILNQCGFAYCKGEVMANNAKWVQSYSGWEKTF
                     EGWVTALEPKDLLHSKIFFDVRAVVDETGLIPKLYQSLWAMLDRQPRFFGLLAHNIQQ
                     LQPPVGVFGDFVCDPKITDRQAFDIKLVMAIFVDFARIYALRQKIGETNTVDRLDALQ
                     RLGVLTNRSHAEMVQAYVMLMKMRIDVQVKALEQNRDPDNYLEPDSLTSLDQKILKEV
                     FEKVKHLQLKLGHDFTGSVNRL"
BASE COUNT          271 a          331 c          329 g          257 t
ORIGIN      
        1 ccccgagacc cttcgggcag tcgtggctga cgatgatatc gacggtcgta tcaggcagag
       61 ccagacagcg atctatgtct tcatcacgaa tcaccgagcc gtgcggagtg atgcgcgggt
      121 ccatgtacga cgcgccaccg aagccgagaa ctctctttcc gcctatctct tgcacgcttc
      181 ccctcggtat atgcgtcaga tgtgaatggt acacccgcgt cagagtctcg aagtgggtga
      241 aatcttcgtg gttcccttcg acgaagtaga agggtcggag aaaccgctcg ttgcgcatga
      301 gaaagtgttc gtgcagagcc ggaccaaaca acccgaagtc gcccagttca atcacggccg
      361 cgatcttttc gtgaaagcgt gcttcggcat gacggatctg ctcgttgacc agaccgtacc
      421 agccgtgcaa atcgctgaga aagagtatca cggaaagacg aggctcacag tctgttcacc
      481 gaaccggtga agtcatgacc cagcttgagc tgtagatgtt tcaccttttc aaagacttcc
      541 ttcagaatct tctgatcgag tgaagtcaga gaatcgggtt cgagataatt gtccgggtca
      601 cgattctgct cgagcgcctt tacctgcacg tcaatgcgca tcttcatcag catcacgtag
      661 gcttgaacca tttcggcatg gctgcggttc gtaagcacgc ccagccgctg cagggcatcg
      721 agtcgatcga cagtattggt ctcgccgatt ttctgacgca gggcgtagat gcgggcaaaa
      781 tcgacgaaga tggccatgac cagcttgatg tcgaacgcct gccggtcggt gatcttcggg
      841 tcgcagacga aatcgccgaa gactccgact ggcggctgca gctgctggat attatgagcc
      901 agtaacccga agaatcgcgg ctgacggtcg agcatggccc agagggactg gtacagcttc
      961 ggtatgagtc cggtttcatc gacgaccgcc cgcacatcga aaaagatttt tgagtgcagc
     1021 aggtctttgg gttcgagggc ggtgacccag ccttcgaagg ttttctccca gccggaatac
     1081 gactgcaccc acttggcatt gttcgccatc acctcgcctt tgcagtaggc gaagccacat
     1141 tgattcaaga tctcacagac catgcccccc aacgagagaa accaggca
//
LOCUS       DAORHD010001613         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_172505_length_1187_cov_3.141343, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001613 DAORHD010000000
VERSION     DAORHD010001613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172505_length_1187_cov_3.141343"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..>1187
                     /locus_tag="PKO06_23925"
     CDS             48..>1187
                     /locus_tag="PKO06_23925"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV72778.1"
                     /translation="MFTIKGMSNADRELLRKYLVSREVPKNAVLFQSDRPAESMFFLE
                     NGAVKLVERKAADGRDDTVVGIIKPGQFCGEEALLAEDARHQVTAVTIEHCLVFEFSR
                     RALQELMAANVTLGTKILLGISRNYRDASFAPQQYGRILVCYSAKDGEGKTSLAVNLA
                     ARLARGGRKTVVLDADLQMGNAHVLLGLNSAPNLSRLVQLEERLNFDRIKLYLQRGHG
                     VDLLAAPDVPQEAELVTRSNLNQILSELTKNYDEVIVDCQSHIDENTLLLWDNADKLL
                     ITVRPDLAGLTRLSRLMRVFSRLDYPKHKFLFVAMKTEPALAEVLPELRRIAGDPLVE
                     MPRLDDTFQKSLSAGRPWSQEPAPEPLEASMNQLMALLQGSPLQTT"
BASE COUNT          238 a          383 c          363 g          203 t
ORIGIN      
        1 ccgacggttg aggcggccct tcaggggctg aagaagtagg gcctgcgatg ttcaccatca
       61 agggaatgag caacgccgac cgagagctgt tgcgcaagta tctcgtcagt cgcgaagtgc
      121 cgaagaacgc ggtgctgttt cagagcgacc gaccggccga gagcatgttc tttctggaaa
      181 acggcgccgt gaagctggtc gaacgcaaag ccgctgacgg gcgcgacgac acggtcgtgg
      241 gcatcatcaa acccggccag ttttgcggtg aagaggctct gttggccgaa gacgcccggc
      301 atcaggtgac cgcggtgacg atcgaacact gcctggtgtt cgaattctcg cgccgcgccc
      361 tgcaggaact catggctgcg aacgtcaccc tgggaaccaa gatcctgctg ggcatcagcc
      421 gcaactaccg tgacgccagc ttcgcgccac agcagtacgg ccgcattctc gtctgctatt
      481 ccgccaaaga cggcgaggga aagacctcgc tggccgtgaa tctcgcggct cgtctggcgc
      541 gcggcggcag aaagaccgtc gtgctcgacg ccgacctgca gatgggcaac gcccacgtgc
      601 ttctggggct caactccgcg ccgaacctgt cgcggctcgt gcaactcgaa gagcgcctga
      661 acttcgaccg catcaagctg tatctgcaac gcggtcacgg ggtcgatctt ctggccgcgc
      721 ccgatgtgcc gcaggaagcc gagttggtga cccgttccaa cctcaaccag atcctctcgg
      781 agctgacgaa gaactacgac gaggtgatcg tcgactgtca gagccacatc gacgagaata
      841 cgctcctgct ctgggacaac gccgacaaac tgctgatcac ggtgcgcccc gatctggccg
      901 gactgacccg cctgagccgc ctgatgcgcg tgttttcgag actcgactac ccgaagcaca
      961 agtttctctt cgtggcgatg aagaccgaac cggccctcgc cgaagtcctg cccgaactgc
     1021 gcaggatcgc cggtgacccg ctggtcgaga tgccgcgtct cgacgacacc ttccagaaat
     1081 cgctgtctgc cggtcgtcca tggtcgcagg aaccggcccc ggagccgctg gaagcctcga
     1141 tgaatcagct catggcgctg ttgcagggaa gccccctgca gaccacc
//
LOCUS       DAORHD010001614         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_172969_length_1185_cov_3.548673, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001614 DAORHD010000000
VERSION     DAORHD010001614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172969_length_1185_cov_3.548673"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="pgi"
                     /locus_tag="PKO06_23930"
     CDS             complement(<1..714)
                     /gene="pgi"
                     /locus_tag="PKO06_23930"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010635529.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV72779.1"
                     /translation="MNAFTTPIDKQIREKKISLTETPEWRALTDHYEKVRDTQMKELF
                     AQDPKRFDRFHAVFEDLLLDFSKNRVTEETMRLLLNLARASGVKEATEAMFAGKKINW
                     TENRAVLHIALRNRSNRPIMVDGADVMPLVNAVLKKMETFSELVRSGFWRGYTDKPIK
                     TVVNIGIGGSDLGPVMITEALKAYADGPAVRFVSNVDGSDFFAKTRDLDPAETLFIVA
                     SKTFTTQETMTNALTARAWL"
     gene            complement(711..>1185)
                     /locus_tag="PKO06_23935"
     CDS             complement(711..>1185)
                     /locus_tag="PKO06_23935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72780.1"
                     /translation="GDLLDPPADTGSSAGPTGGASIRLDKSTQGQKTFVMPCDGQVKI
                     TACLTTYGPASLEVTHAAKGGAPEVLMSWERRDDKDPSPLLVREQPVPTSINEGKPGD
                     FTPVPASQTFPAREGDTFVLRLQGKFGNANPSLVLEALVPSDSSNHSSSTGETSQ"
BASE COUNT          214 a          339 c          360 g          272 t
ORIGIN      
        1 cagccacgcg cgggcggtga gggcgttggt catcgtctcc tgcgtggtga aggttttcga
       61 cgcgacgatg aacagcgttt cggcggggtc gagatcgcgc gtcttcgcga agaaatcact
      121 gccgtcgacg ttcgagacga agcgcacggc tgggccgtcg gcatacgcct tgagggcttc
      181 ggtgatcatg accgggccga gatctgagcc gccgataccg atgttcacca ccgtcttgat
      241 gggtttgtcg gtgtagccgc gccagaatcc gctgcgtacc agttcggaaa acgtctccat
      301 ctttttcaga accgcattga ccaggggcat gacatcagcc ccgtcgacca tgatcggccg
      361 attcgatcgg ttgcgcaacg cgatgtgcaa caccgcgcga ttctccgtcc agttgatctt
      421 cttgcctgca aacatcgcct ccgtcgcttc cttgaccccg gaggcacggg ccagattcaa
      481 cagcagacgc atcgtctcct cggtcacccg attcttcgag aagtcgagca acagatcttc
      541 aaacacggca tgaaaccgat cgaatcgttt tggatcctgc gcgaacagct ctttcatctg
      601 cgtgtcgcgc actttctcat agtggtcggt gagcgctcgc cattcggggg tctcggtcag
      661 gctgatcttc ttctcgcgga tctgcttgtc gatcggtgtt gtgaacgcgt tcattggctt
      721 gtttctcccg tggaagatga atggtttgaa gaatcgctgg gaacgagagc ttcgagaacg
      781 agagacgggt tggcgttgcc gaatttgccc tgcagtcgca gcacaaaagt gtcaccttca
      841 cgtgccggaa aggtctggga cgctggcact ggagtgaaat cgcccggttt tccctcgttg
      901 atcgaagttg gaacgggttg ttcgcgtacg agcagtggtg aggggtcttt gtcatcgcga
      961 cgttcccagc tcatcagcac ttccggtgcc ccaccctttg ccgcgtgagt gacctcgagt
     1021 gaagccgggc catacgtggt cagacaggcg gtgattttca cctgcccgtc gcacggcatg
     1081 acgaaagtct tctgaccctg cgtcgacttg tcgagacgaa tcgaggcccc tcccgttggt
     1141 cctgcgctgg agccggtgtc tgccggagga tcgaggaggt cgccg
//
LOCUS       DAORHD010001615         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_173992_length_1181_cov_4.174067, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001615 DAORHD010000000
VERSION     DAORHD010001615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173992_length_1181_cov_4.174067"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1181
                     /locus_tag="PKO06_23940"
     CDS             37..>1181
                     /locus_tag="PKO06_23940"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNV72781.1"
                     /translation="MRLRLVCQDLGPTFIKFGQILSTRPDLIPPDVANELKNLQDNIP
                     PFSFVHVKEQIESELGKPLSELFETFEEAPLGTASIGQVHAARLKDPTVGSVEDVVVK
                     IQRPQIRGTIESDIDLMRLLAKSLEKRVKQLKTLDLTGILQEFELAIIKELDYSHERR
                     NGTRFYAAFTGNPDVVIPRVYRSHSAKKVLTMQRIKGVKISDAETVGADRRVLARVAL
                     RAVLQMVFQNGFFHADPHPGNIFALAGNRVAFLDLGMVGRLSDEMRLHLSDLFIALMQ
                     RDTDEIARILCVMGIRQGRVNRASLQRDVIDIMDKVIGVPLEEIQFSEIVKDLMESAR
                     RHQIKIPNEYTLMGKALLTVEGVGKNLDPTIDVEAEITPYIRGLIKDR"
BASE COUNT          260 a          318 c          364 g          239 t
ORIGIN      
        1 gcaacgagtg ctcgaaacga ggatacgaca cctgcagtgc gcctgcgtct cgtctgccag
       61 gacctcggac cgacgttcat caagttcggg cagattctga gtactcgacc cgatctcata
      121 ccacccgacg tcgcgaacga gttgaagaac ctgcaggaca acattccgcc gttttcgttc
      181 gttcacgtga aagagcagat cgaatcggag ctcggcaagc cattgtcgga actcttcgaa
      241 accttcgagg aggcacccct cggtacggct tcgatcgggc aggttcacgc cgcgcggttg
      301 aaggacccga ctgtgggatc ggtcgaagac gtggtggtca agattcaacg cccgcagata
      361 cgtggcacca tcgagagtga catcgacctc atgcgcctgc tggccaagtc tctcgaaaaa
      421 cgggtgaagc agctgaagac cctcgatctc accggaattc tccaggagtt tgagttggcg
      481 atcatcaagg aattggacta ctcgcatgag cgccgcaatg ggacaagatt ttacgcggcg
      541 tttaccggga accccgatgt cgtcattccg cgagtgtacc ggtcgcattc ggcgaagaag
      601 gtcctgacga tgcaacgcat caagggcgtg aagatctccg acgcggaaac ggtgggggct
      661 gatcggcggg tgctggcacg cgtggcactg cgagcggtgc tgcagatggt ttttcagaac
      721 gggttctttc atgccgaccc gcaccccgga aacatctttg ctctcgcggg aaatcgggtg
      781 gcgtttctcg atctgggcat ggtcggacgt ctgagcgacg agatgcgtct gcatctgagt
      841 gatctgttca ttgcgctgat gcagcgtgat accgatgaaa tcgcgcgcat tctctgtgtg
      901 atgggcattc gccagggccg ggtgaaccgg gcctcgctgc agcgtgatgt catcgatatc
      961 atggacaagg tgatcggcgt gccgctcgag gagattcagt tcagtgagat cgtgaaagac
     1021 ctcatggaaa gcgcgcggcg acaccagatc aagattccga acgagtatac gctcatggga
     1081 aaggcgctct tgaccgtcga gggagtcggc aagaacctgg acccgacgat cgatgtcgag
     1141 gccgaaatca ccccgtacat tcgtggtctg atcaaggatc g
//
LOCUS       DAORHD010001616         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_174274_length_1180_cov_3.560000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001616 DAORHD010000000
VERSION     DAORHD010001616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174274_length_1180_cov_3.560000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PKO06_23945"
     CDS             complement(<1..232)
                     /locus_tag="PKO06_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72782.1"
                     /translation="MKTLTRWLGVAALLVFPAWGGSTLQAQEFPGDELPQLSLEGSAY
                     DTRSENLPLPDAPLPDVRSGRTSEDVSFYDPSG"
     gene            431..>1180
                     /locus_tag="PKO06_23950"
     CDS             431..>1180
                     /locus_tag="PKO06_23950"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV72783.1"
                     /translation="MRKTFVLDTNVLLHTHNSLFSFKDNEVVVPLGVIEELDRFKGHN
                     DERGREARSVSRELDKLREKGPLTQGVEINNGAGLLRVMLDTDIEIPQGFDKNKVDNR
                     IIACALDLKKAGKNVFLVSKDINARIKCDAIGVPAEDFETNKVDVDELYTGWRELVVT
                     ARDIDVFQKNQVLPCPVQGMYANEFVLLKAAENENKTALAKYKADSNNLVSLYHVSSK
                     PYSVEPQNLQQKFAIELLLCNEIQLVTMVGRA"
BASE COUNT          273 a          342 c          317 g          248 t
ORIGIN      
        1 ctccggaggg atcgtagaag gagacatcct cactcgtgcg accgcttcgc acatccggca
       61 gcggcgcatc aggcaatgga aggttctccg atcgcgtgtc gtaggctgat ccttccaggc
      121 tgagctgtgg caactcatcc ccagggaact cctgcgcctg gagtgtcgaa cctccccagg
      181 ccggaaaaac cagcagggcg gcaacaccga gccatcgtgt caacgtcttc atcggacagt
      241 tcctttccgg ccgaggcggc cgaggaaaga tatgattccg catctatcat cggcgaggtg
      301 tggcaaaatc ttaatgtttt tccacagact cgctgaccca gctgaccccg tgccgatcgc
      361 aggtttgact ccccccgctg cctgtggtat cgtttgttta tcctgtctca gatccaggga
      421 aggggacttc atgcgcaaaa cctttgtcct tgacaccaat gtactgcttc atactcacaa
      481 ctcactcttt tcattcaagg acaacgaggt ggttgtgcca ctcggggtca tcgaagagct
      541 cgaccgattc aaggggcaca atgatgaacg cggccgcgag gcacgttcgg tctcacgcga
      601 actcgacaaa ctgcgcgaaa aaggtcccct gactcagggc gtcgagatca acaacggtgc
      661 cggcctgctg cgagtcatgc ttgataccga tatcgagatt ccccagggct tcgacaagaa
      721 caaggtcgac aaccgcatca tcgcctgcgc cctcgacctg aagaaagccg gtaagaacgt
      781 gtttctggtt tccaaagaca tcaatgcccg cattaaatgc gacgccatcg gcgtgccggc
      841 cgaggatttc gaaaccaaca aggtcgacgt tgacgagttg tacaccggat ggcgcgaact
      901 ggtggtcacc gcgcgcgata tcgacgtgtt tcagaagaac caggttcttc cctgccccgt
      961 gcagggcatg tatgcaaacg agtttgtgtt gttgaaggcc gctgaaaacg agaacaagac
     1021 cgcactcgcg aagtacaagg ccgactccaa caatttggtt tctttgtatc acgtctcgtc
     1081 gaaaccctac agtgtcgaac cccagaatct gcaacagaag ttcgccatcg agctgctgct
     1141 gtgcaatgag attcagctgg tcaccatggt cggtcgggct
//
LOCUS       DAORHD010001617         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_174313_length_1180_cov_2.772444, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001617 DAORHD010000000
VERSION     DAORHD010001617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174313_length_1180_cov_2.772444"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1180)
                     /locus_tag="PKO06_23955"
     CDS             complement(<1..>1180)
                     /locus_tag="PKO06_23955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72784.1"
                     /translation="ESNRLVQGRFFCGWFAPESREGLRPTELVTRSGLDQTLRAMNAG
                     RSFAEWQSSWLVTHSTESGERLFAVARQPDNMDERRLQWQRWLLVGLLIGLFVATWCH
                     VSLRGLRARLIWQYAIAGGFPLLVFLSMVVIDRENRVFLLEQEFCEGHLAFLSKFDAD
                     YHSVFVPALRHYETVYQKISADPSRLVTQVTPGLEKLIDRLRGLITRLVLVDRREQIL
                     FFGRYGASPTVEGQETLPHGALSVLQNVNLEAPSSSTAGLNNPIMNMLVQTKYAPQWI
                     DEDGILKNNHVGNESVLSYFRLFPINNASYGAILMATHDGRKGQIRYLQSRLGKSLSR
                     FGRRPQVWALPAVPGAWPAFPDESIDRDPLVRKLKDLVVSTGLPQHQRLWLKNRMMLV
                     S"
BASE COUNT          268 a          371 c          320 g          221 t
ORIGIN      
        1 cagaaaccag catcatccga ttcttcaacc acagtcgctg gtgctgcggc aacccggtcg
       61 aaaccaccag gtctttcagt ttgcgcacca gcggatcacg gtcgatcgac tcgtcgggaa
      121 acgccggcca ggctcccgga accgccggca acgcccacac ctgtggtctt cttccgaagc
      181 gcgagagcga ttttcccaac ctggactgca ggtagcgtat ctgccctttg cgtccatcgt
      241 gcgtcgccat cagaatggca ccgtaactgg cgttgttgat cgggaaaagc cggaagtacg
      301 acaacacgct ctcgttgccc acgtgattgt ttttcagaat tccgtcttca tcgatccact
      361 gaggggcata cttggtctgc accagcatgt tcataatggg attgttcaag cccgccgtcg
      421 aactgctggg cgcttcgagg ttcacgttct gcagaacgct cagcgccccg tgcggcaggg
      481 tttcctgacc ctcgaccgtc ggtgatgccc catagcggcc gaaaaagaga atctgctcgc
      541 gacgatcgac aagaaccagt cgagtgatca agccacgcaa ccggtcgatc agtttttcca
      601 gacccggggt tacctgtgtg accagtcgcg acggatcggc gctgatcttc tgatacacgg
      661 tttcatagtg ccgcaaggca gggacgaaca ccgagtgata atcggcatcg aacttggaaa
      721 gaaaggccag atggccctca cagaactcct gttccaacag gaagacccgg ttctcgcgat
      781 cgataaccac catcgagaga aagaccagca gcgggaaacc gccagcgatg gcatactgcc
      841 agatcaggcg cgcccgcagg ccgcgcaatg aaacatggca ccaggtggcc acaaacagac
      901 cgatcagcag cccaaccagc agccagcgtt gccactgcag acggcgttca tccatgttgt
      961 caggctgacg tgccaccgca aacagacgtt ctcctgattc ggtcgagtgg gtcacgagcc
     1021 acgacgactg ccactcagcg aacgatcgtc cggcgttcat ggctctcaat gtctgatcca
     1081 aacccgatct cgtcaccagt tcggtgggac gcaggccctc gcggctctcg ggggcaaacc
     1141 agccgcagaa gaatcgcccc tgcaccagtc gattcgattc
//
LOCUS       DAORHD010001618         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_174596_length_1179_cov_2.802491, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001618 DAORHD010000000
VERSION     DAORHD010001618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174596_length_1179_cov_2.802491"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..510
                     /locus_tag="PKO06_23960"
     CDS             250..510
                     /locus_tag="PKO06_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNV72785.1"
                     /translation="MEVLRVASSSNPKSVAGAIAAVLEHDTKVELLAVGAGAVNQTVK
                     AIAVTRGYVAPKGIDLITVIAFAKIQIDGNEKTAIKFIVEAR"
     gene            542..>1179
                     /locus_tag="PKO06_23965"
     CDS             542..>1179
                     /locus_tag="PKO06_23965"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72786.1"
                     /translation="MNRTTPGLEQPPTTLVSFWSMIRRPALIAIFCSSLIGSPPSLQA
                     QDRIDPAPLLKTLQEDPDDRNALLQMGLYYTMEKNFGKAVEMYFRLLKVDPNNFHAYN
                     NLGIIYKKVGQLKDSLHCYGQAMKLDPSNPWVPYNMGLAYESMGRMQEARESYGKALS
                     LNPDFAQALQRLRDLSGSEQTAPLPPPTSILVADNSDASPRALTAGDLGKQPP"
BASE COUNT          272 a          346 c          317 g          244 t
ORIGIN      
        1 gcacgtcgtg atgcagacga aggttgatca cctgatccag tgcggcatcg tcgaatcatt
       61 cgggttcggc ggccaaaatg cctgtgttgc cttcaaacgg gtttagtgtc ataatggtac
      121 tgaacggggt ttgagcgtat cacctcgtcg ttctttcgtc cccgagagga actcggtttg
      181 ccaaatcact gaagggttac taccatggag tcagaggaat cattcgtcta cacatccgag
      241 gagaggtcaa tggaagttct gcgcgttgca tcatcatcga accccaaatc ggtggccggg
      301 gccatcgcgg cggttcttga gcacgacacc aaggttgaac ttctggctgt cggtgccggt
      361 gcagtcaacc agaccgtgaa agccatcgcg gtcacgcgcg gatacgtcgc ccccaagggc
      421 atcgatttga tcaccgtcat cgccttcgcc aagattcaga tcgacggcaa cgagaaaacg
      481 gctatcaaat tcatcgtcga agcgcgctga acgcttcgcc cccagatcag gcacggatgc
      541 catgaatcgt acgaccccag ggctggagca gccaccgacg actcttgttt cgttctggtc
      601 gatgattcgt cgcccggcgt tgatcgcaat tttctgttcg tcgctgatcg gcagcccccc
      661 ctcattgcag gctcaagacc gcatcgaccc cgcaccgctg ttaaaaacac tgcaggaaga
      721 ccccgatgac cgcaacgctt tgctgcagat ggggttgtac tacaccatgg agaaaaactt
      781 cggcaaagcg gtcgaaatgt actttcgtct gctgaaggtt gatcccaata actttcatgc
      841 ctacaacaat ctgggtatca tctacaagaa agttggccaa ctgaaagaca gcctgcactg
      901 ctacggacag gccatgaaac tcgacccgtc gaatccttgg gttccgtaca atatgggatt
      961 ggcctacgag tcgatgggcc gcatgcagga agcacgagaa agctatggga aggccctttc
     1021 gctgaacccg gacttcgctc aggccttaca gcgactgcgc gatctgtcag gaagcgaaca
     1081 aacggctcct cttccgccgc cgaccagcat tctggtcgcc gacaactcgg acgcaagtcc
     1141 gcgcgcgctg accgcaggcg acctcggcaa gcagccccc
//
LOCUS       DAORHD010001619         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_174608_length_1179_cov_2.652135, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001619 DAORHD010000000
VERSION     DAORHD010001619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_174608_length_1179_cov_2.652135"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..775)
                     /locus_tag="PKO06_23970"
     CDS             complement(122..775)
                     /locus_tag="PKO06_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014777759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV72787.1"
                     /translation="MAGSPRSARETGQTFTYGDYRAWPPEERWELIDGVPHAMTAPST
                     NHQEVLLKLGRLFDEQLEGKPCRPFIAPVDILLPKGDEQDDEVVHVLQPDLVIFCDRD
                     KIRPKNCRGAPNLVVEILSTSTSSFDHLKKKSLYERFGVREYWLVHPDEGVITIYQRE
                     ASGLFGASRVVSRDDTVEVAILPGVTIEFPKVYARIPNPYVAKEPPARAYSAQSATE"
     gene            complement(889..1152)
                     /locus_tag="PKO06_23975"
                     /pseudo
     CDS             complement(889..1152)
                     /locus_tag="PKO06_23975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554004.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT          246 a          299 c          366 g          268 t
ORIGIN      
        1 cgaatctccg gaaacgaggt cagaacctct cgtagctgat catcgagatg gcactgctga
       61 tcatggttca ctcgttgctt ccccttgtct acgaatatcg cgtgtttgtc tctgcaaggg
      121 attactcggt cgcagattga gcggaatagg cgcgggcggg cggttctttg gccacgtagg
      181 ggttgggaat gcgcgcgtag accttcggga attcgatggt cacgccgggc agaatggcga
      241 cctcaaccgt atcgtcgcgc gagacaaccc gcgaggctcc gaagagcccc gaagcttcgc
      301 gctgatagat agtgatgacg ccttcgtcgg ggtggaccag ccagtactca cgcacaccaa
      361 accgctcgta gagagatttc ttcttcagat ggtcgaagga tgaggtcgat gtcgagagga
      421 tctcgaccac gaggttgggt gcgccccgac agttcttcgg ccggattttg tcgcgatcgc
      481 agaagatcac gagatcgggt tgtaagacgt gaacgacttc atcgtcttgc tcgtcgccct
      541 ttggcaaaag aatgtcgacc ggggcgatga agggtcggca aggcttgccc tcgagctgtt
      601 catcgaacag cctgcccagc ttaagaagaa cctcctggtg gttcgtcgag ggggcagtca
      661 tcgcgtgcgg aacgccgtcg atcagctccc agcgttcttc gggcggccag gcgcgatagt
      721 ctccgtacgt gaacgtttga ccagtttcgc gagcacttct gggagatccg gccatagtct
      781 acgtctccat ggtggggtgt cgatttcgtc tgctacgatg atagcataac cggcacgatc
      841 atcgaacggc atgaaggcgc gtcagagatt gattgaacga aaaggagctt aggtgtagtg
      901 aaaacggcac tgggcgatga tcagagagct gttgcgcacg gtgtagacca ggcggtgttc
      961 gtcagtgatt cgtcgtgaac agtagccgga aaggttgcct ttcagcattt cgggtttgcc
     1021 gaggccgtcc ccggcgtgcg ggttgcggag aatttcgtgt ctgagcagat ttgtccgctt
     1081 taaaaccttc tgactggtcg tcggtcagta aagatagtcc ccccaaccga tctcagacaa
     1141 cgcgatgatc acgggtcgat gagtttgtat tgctgaaac
//
LOCUS       DAORHD010001620         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_175444_length_1176_cov_2.181980, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001620 DAORHD010000000
VERSION     DAORHD010001620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175444_length_1176_cov_2.181980"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..321)
                     /locus_tag="PKO06_23980"
     CDS             complement(4..321)
                     /locus_tag="PKO06_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72788.1"
                     /translation="MVDAARTLGARVIVIAPDGALASKADAALTTGGGLNAVFPVEGM
                     DRPFGPVSGIVNMLVFNMLQAETAAKLIASGKKPTVLPAEYLPGGREKRIEALRMFDE
                     HKC"
     gene            328..>1176
                     /locus_tag="PKO06_23985"
     CDS             328..>1176
                     /locus_tag="PKO06_23985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72789.1"
                     /translation="MVASTPVLAGASCSFSVTARDSGNNLLTGYTGTIGLTHDAIGET
                     NLPEIYTFSLADTGVHVFTASAAARFTRTGAFRIRVEDSGRIGFSNAITVNPDTAGVR
                     VSVIPEEYTVELGESFSYRVALEDRFGNPLSGYTGAININYPGSAAPSGPLTYTFQSF
                     ENGSHRFLEQVYPTLLGTFSAWVTEPVSGVVASSAEIQVISGDTLNFDVEPASFTIYA
                     GDTITFNVTASDSAGNLNQGYVGSVRFTATDPLALVPPDYALTLGQGTFDTTFYTAGR
                     YQYTVRD"
BASE COUNT          223 a          383 c          312 g          258 t
ORIGIN      
        1 cgttcagcat ttgtgctcgt cgaacatccg gagcgcttct atcctttttt ccctgccgcc
       61 gggaagatac tcggcaggca aaacggtcgg ctttttcccc gatgcgatga gtttcgcggc
      121 ggtctcggcc tgcagcatgt tgaacacgag catgttcacg atccccgata cgggtccgaa
      181 cggccggtcc attccctcga ccgggaaaac ggcattcaat ccgccgcccg tcgtaagcgc
      241 ggcatcggct tttgatgcca gggcgccgtc cggggcgatg acgatcacac gcgcgccaag
      301 agttcgcgcg gcatcgacca tttcgatgtg gttgcttcga ccccggtcct ggctggagcc
      361 agttgttcct tctccgtcac cgcccgcgac tctgggaaca atctgctgac tggctacaca
      421 ggcacgatcg gactcacaca cgatgcgatt ggcgagacca acctgcccga gatctacacc
      481 ttcagtctgg ctgatacagg ggtgcatgtc ttcaccgcct ctgccgccgc ccgttttact
      541 cgtacgggag cattccgtat ccgcgtcgaa gattctggac gaatcggatt ttcgaatgcc
      601 atcacggtca atccggatac ggctggcgtg agagtgagcg ttatccccga agaatatacg
      661 gtcgaactcg gcgaatcttt cagctatcga gtcgcgctcg aagaccgttt cggaaacccg
      721 ctgagcggat acaccggagc gatcaatatc aactaccccg gttcagcggc accttcaggg
      781 cccctgacct atacctttca gtccttcgaa aatggatcac accgttttct cgagcaggtc
      841 taccccactc tgctcgggac cttctccgcc tgggttacag agccagtctc cggggttgtg
      901 gcctcatccg ccgagattca ggtgatcagc ggcgacaccc tgaacttcga cgtcgaaccc
      961 gcctcgttca ctatttacgc cggcgacacg atcaccttca atgtcaccgc ctccgattcg
     1021 gccggtaacc tgaatcaggg atacgtcgga tcggtacgat tcacggccac cgaccccctc
     1081 gcgctggtcc cgcctgatta tgccctgact ctgggccagg gcactttcga tacgactttc
     1141 tacaccgcgg ggcgttacca gtacaccgtg cgtgac
//
LOCUS       DAORHD010001621         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_175849_length_1174_cov_3.505809, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001621 DAORHD010000000
VERSION     DAORHD010001621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175849_length_1174_cov_3.505809"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..425)
                     /locus_tag="PKO06_23990"
     CDS             complement(24..425)
                     /locus_tag="PKO06_23990"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNV72790.1"
                     /translation="MFKVDESRGIPIYQQIVEGFKRDILAGTWKADAKLPSIRDLALE
                     LKVNPNTIARAFQDLEGQGVIYFKRGLGAFVSPQSREDRMKQAKLELAQHLQQIIELA
                     RQFGIAPDELKSMLDGQLRNSGTSARKEEDA"
     gene            complement(623..>1174)
                     /locus_tag="PKO06_23995"
     CDS             complement(623..>1174)
                     /locus_tag="PKO06_23995"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNV72791.1"
                     /translation="NVPGALTRIDLSRGQTSVRESLATQTVGSLLLLSGFVILARDLA
                     HQRWRDLIQAGRPLPDYLRQYPIWYAGPARTPPGMPIGSVGPTTSGRMDQCFAQLMAQ
                     GGSWLTIGKGQRSATAVDACRAYGGWYLGTIGGVAALLAREHVSSCELIDHADLGMEA
                     VYRVRLRDLPVFVVIDHHGNHMY"
BASE COUNT          245 a          326 c          348 g          255 t
ORIGIN      
        1 cagttccgag atggtgatgg cgttcatgcg tcttcctcct tgcgggctga ggtgccgctg
       61 ttgcgcagtt gtccgtcgag catgctcttc agttcatccg gagcgatgcc aaactggcgg
      121 gccagttcaa taatctgctg aagatgttgt gcgagttcga gttttgcctg tttcatgcgg
      181 tcctcccgtg attgcggcga gacaaacgct cccaggccgc gtttgaagta gatgaccccc
      241 tgcccttcca gatcctgaaa ggcgcgggcg atcgtgttgg gattcacttt gagttcgaga
      301 gccagatcgc ggatcgatgg cagtttcgcg tcggccttcc aggtgccggc cagaatatcg
      361 cgtttgaagc cttcgacgat ctgctggtag ataggtatgc cgcgggactc gtcgactttg
      421 aacacggatc tcacctcctt gatagatgtt cacgcaccgg tgtcgctgtg ttttagtgta
      481 ttaccttact aatacactaa ttcgttcggt catgaaagtc aaggaaagtt ttttcgaggt
      541 atcaatgacg atcaggaacg aggcatgaaa acgcctgtca ctactgcatc tgcatacgtt
      601 gacgacgagg aaaaatccgt ggtcagtaca tgtggttccc gtgatgatca atgacgacaa
      661 acaccggcag atcgcgcaac cgaacccggt ataccgcttc catgcccaga tcggcgtgat
      721 cgatgagctc gcaggagctc acgtgctcac gagccagcag agccgcgacc ccgccgatgg
      781 tgccgagata ccaaccgccg tacgcgcgac aagcatcaac agccgtcgct gagcgctggc
      841 cttttccgat cgtcagccac gagccaccct gagccatcag ttgggcgaaa cattgatcca
      901 tgcgcccgga ggtggttggc ccgacactgc cgattggcat gccaggtggg gtacgggccg
      961 ggccagcgta ccagatcgga tactgtcgaa gatagtctgg caggggtcgt ccggcttgaa
     1021 tcagatcgcg ccaccgctgg tgcgcgagat cgcgggccag aatgacgaac ccagaaagga
     1081 gcagcagcga gccgaccgtc tgtgtcgcga gagactctct caccgatgtc tggccccggg
     1141 aaagatcgat gcgcgtgagt gctcctggga catt
//
LOCUS       DAORHD010001622         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_175861_length_1174_cov_3.352994, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001622 DAORHD010000000
VERSION     DAORHD010001622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175861_length_1174_cov_3.352994"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1174
                     /locus_tag="PKO06_24000"
     CDS             <1..>1174
                     /locus_tag="PKO06_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72792.1"
                     /translation="NPGGPATLSPQVLDDLKNTIVQECLTTLHKRRNKYIRKGLWKWI
                     EREIVDPNTHFFLVILSCIYQGEVGEVLSRRFKSCEDYSKNPEVIADTIFSRDAGVAS
                     EIVKNSDRHRRALKKFLECFAATPPFDYLRSLFLKEFRTNHDSIKSRTAVFDTLKELM
                     QRCGFEGEKEVQYPLEILDELGIFQGFIMGNFTELRVDNAGKKLRQLVPGMEWTPVEI
                     YRLRDQLATRLDLPPSEFNLNAFLPQKFISSRKDVSSLLQPQPRSPRERQAQPPRQHP
                     VPPAPPVPPQSSDGSLNAPMSEPARPPARSNAPVQGLFGSVAAPIPETAPAAPVHPTT
                     PAEPVMAPRPPRPQPPMGTHHQRPPAPAFVPTPPKPNVDPNALAIISGQQRRPMGPE"
BASE COUNT          261 a          397 c          285 g          231 t
ORIGIN      
        1 caacccgggc ggaccagcaa ccctgtctcc acaagttctc gatgacctga aaaacaccat
       61 cgtgcaggaa tgcctgacca ctctgcacaa gcgacgcaac aagtacattc gcaaaggtct
      121 ctggaaatgg atcgagcgcg agatcgtcga ccccaacacg cacttttttc tcgtcattct
      181 ctcgtgcatc tatcaggggg aggtcggtga agtcctgagc cgtcgcttca agtcctgtga
      241 agactacagt aaaaaccctg aagtcattgc tgatacgatc ttttcgcgtg acgccggagt
      301 cgccagcgag atcgtcaaaa actctgatcg acaccgccgc gcactcaaga aattcctcga
      361 atgtttcgcc gcaacgcccc cgtttgacta ccttcgcagc ctttttctga aagaatttcg
      421 caccaatcat gactcgatca agtcccgcac ggccgttttc gacaccctga aggaactcat
      481 gcaacgatgc ggattcgagg gcgagaaaga agtgcagtac ccgctcgaga ttcttgacga
      541 actcggcatc tttcaaggat tcatcatggg gaatttcacc gaactgcgcg tcgacaatgc
      601 cgggaagaag ctacgacagc tggttcccgg tatggaatgg accccggtgg aaatctaccg
      661 tctgcgtgat cagctggcca cgcggctcga tctgccgcca tcagagttca atctcaacgc
      721 ctttctgcct cagaaattca tcagttcacg caaagatgtg tccagtctgc ttcagccaca
      781 acctcgctca ccgcgcgaac gacaggcaca gcctccgcga cagcacccgg ttccaccggc
      841 tccaccggtc ccgcctcaaa gttccgatgg gtcgctgaac gctcccatgt ccgagcccgc
      901 acggccgccg gcccgttcga acgctcccgt ccaggggctg ttcggttcgg ttgccgcccc
      961 cattcctgag acggcaccag cagcaccagt tcatcccacc acgccggcag agccggtcat
     1021 ggccccacgt ccgccgcgcc ctcagcctcc catgggcact catcatcaac gccccccggc
     1081 cccggctttt gttccgacac ctccgaaacc gaatgttgat ccgaatgctc tggccattat
     1141 ctcggggcaa caacgccgac cgatgggccc cgaa
//
LOCUS       DAORHD010001623         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_175937_length_1174_cov_2.641644, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001623 DAORHD010000000
VERSION     DAORHD010001623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175937_length_1174_cov_2.641644"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1070
                     /locus_tag="PKO06_24005"
     CDS             108..1070
                     /locus_tag="PKO06_24005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV72793.1"
                     /translation="MTPFVSIVVVGRNVERTLAECLDSISQLNYPTFEILYIDDGSED
                     RSIAIARRFSSVQQLASQGRGPSAARNLGIRQAHGDFVAFTDADCTVDRNWLKELVEQ
                     LSSHPEVAGVGGRQQPSLRACDFELELYAFMESVSSLIDYVRSPAPDAAIPVAHNPSC
                     NVLYRRDALMHIGGFREHLWPGEDVDLDYRFTRNGQSLLYHAKAIVFHDKPKNLSDWI
                     AMMNRYGWAQGVLCRIHGVFRRLHFVALLTPLLLLFSSRWLRLKVSPIVAGVSIVTLL
                     SQRLQIAITGSTAWLIGFLTGVTVPPDWTKQQQRDLQPLITRST"
BASE COUNT          237 a          332 c          311 g          294 t
ORIGIN      
        1 cgtccgtcct tcatgctgcg ctttctctgg aacaccagaa acttcaagga cttgtatcgc
       61 aagattcgag gcgcacggaa cttcctttcg tatctggcgg gtttttcatg acacccttcg
      121 tttcgattgt tgtcgtggga agaaatgtcg aacgcaccct ggctgagtgt ctcgactcga
      181 tctctcagct gaactatccc acgttcgaga tcctgtacat cgatgacggt tctgaagacc
      241 gatccatcgc cattgccagg cggttcagct cggttcaaca gctcgcttct caaggtcgcg
      301 gcccatcagc agcccgcaat ctgggcattc ggcaagcgca cggagacttt gtcgccttca
      361 cggatgccga ttgcacggtc gaccggaatt ggctgaagga gctcgtggag cagttgtcct
      421 cgcacccaga ggtagcaggg gttggggggc gtcagcagcc ctctctgaga gcctgtgact
      481 tcgagctcga gctgtatgcc ttcatggagt ccgtttcttc actgatcgat tatgtccgtt
      541 ctccggcacc agatgccgcc atcccggttg ctcataatcc atcgtgtaat gttctgtacc
      601 gacgcgatgc gctcatgcac atcggtgggt tccgtgagca tctctggccc ggtgaagatg
      661 tcgacctgga ctatcgtttc accagaaacg gacaatcgct tctgtaccat gccaaagcga
      721 tcgtctttca tgacaaaccg aagaacctgt cagactggat cgccatgatg aaccgatacg
      781 ggtgggctca gggagtgctc tgccgtatcc atggagtttt caggcggctg cacttcgtag
      841 ctttgttgac acccttgctt ctgctgtttt ccagccgatg gcttcgtctg aaggtgtcac
      901 cgattgtggc aggggtaagt attgtgacgc tgctttcgca aaggcttcag atcgctataa
      961 ctggatcgac agcatggttg atcggctttc taactggagt cactgttccg ccagactgga
     1021 caaaacaaca acaacgagac cttcagcctc ttatcacgag gtcgacatga gtaccttttt
     1081 ccccgccacg gccgtcattc tctgcggagg taagggaacc cgcctctggc cccagacgtt
     1141 gatccatcag aaagctatgc tccacgtgca aggc
//
LOCUS       DAORHD010001624         1173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_176244_length_1173_cov_2.263864, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001624 DAORHD010000000
VERSION     DAORHD010001624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176244_length_1173_cov_2.263864"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PKO06_24010"
     CDS             <1..869
                     /locus_tag="PKO06_24010"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV72794.1"
                     /translation="EGGIGLIAGSGLTDEELREEIGIARSLANGGIVGINTLVAASRF
                     IGVMKTAAEAGIDMLVAGAGFSRELFEIGRRHGIPVVPIVSTARLAVLAESLGASAVI
                     VEGKEAGGHLGTTKSSRVLYPLVKEAVKIPVIIAGGITDRADLLEVLNMKVDGVQVGT
                     RFAASHESNVSAEFKERYLQAKEGDVFKIHSPVGLPGNALMTPFYERVQRGEVDPISC
                     DGCLKRCSHSFCIVRALINAKDGKLDQGLVFAGEHVYKIKEILSVPEIFKLFFDGLEF
                     IDETTTPGGTPS"
     gene            866..>1173
                     /locus_tag="PKO06_24015"
     CDS             866..>1173
                     /locus_tag="PKO06_24015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNV72795.1"
                     /translation="MNRVVVTGLGAVTPIGTGCEAFAKSLREGKSGVAPISCFDASKH
                     SVRIAAEVRDFDPTPYINRKEIRRHDRYSQFAMAAAHMAVQQAGLKAEGHDARRIGTM
                     V"
BASE COUNT          233 a          345 c          357 g          238 t
ORIGIN      
        1 gtgaaggcgg tatcggcctg atcgcgggtt cgggtctcac cgacgaggag ctgcgcgaag
       61 agatcggtat cgcgcgcagt ctggccaacg gcggcatcgt tggaatcaac acgctggtcg
      121 ccgcatctcg tttcatcggc gtcatgaaaa ccgctgctga agcaggaatc gatatgctgg
      181 tggccggtgc cggcttttct cgcgaattgt tcgagatcgg ccggcggcac gggattcccg
      241 tcgttcccat agtctcgacg gctcgtctgg cggttctcgc cgagagtctc ggcgcaagtg
      301 ccgtcatcgt tgagggcaag gaagccggcg ggcacctggg cacgacgaaa tcatcgcgag
      361 tcctgtatcc gctggtcaaa gaggcggtca agatcccggt catcattgcc ggggggatta
      421 ccgatcgcgc ggatctcctc gaggttctga acatgaaggt ggatggcgtt caagtgggaa
      481 cgcggttcgc cgccagtcat gaatccaatg tttcagccga attcaaggaa agatatctgc
      541 aggccaaaga gggggatgtt ttcaagatcc acagccccgt gggcctgccc ggaaacgcgc
      601 tgatgactcc gttctacgaa cgcgttcagc gcggcgaggt cgacccgatc tcctgcgacg
      661 ggtgtctcaa gcgctgttcg cacagcttct gcatcgtgcg cgccctcatc aatgcaaaag
      721 acggaaagct tgatcagggg ctggtttttg ccggcgagca cgtgtacaaa atcaaagaga
      781 tcctttccgt acccgaaatt ttcaagctgt tcttcgatgg ccttgagttc atcgatgaaa
      841 ccacgactcc aggaggcact ccctcatgaa tcgcgtcgtc gtcaccggcc ttggcgccgt
      901 cacccctatc ggaaccggct gcgaagcctt tgccaagtct ctgcgcgaag gcaagagcgg
      961 ggttgccccc atcagttgtt ttgacgccag caagcactcc gtgcgcattg cggccgaggt
     1021 tcgtgacttc gacccgactc cgtacatcaa tcgcaaggag atccggcgac acgaccgtta
     1081 ttcgcagttc gccatggccg ccgcgcacat ggcggtccag caagccggcc tgaaggccga
     1141 aggtcatgat gcccgccgca tcggcaccat ggt
//
LOCUS       DAORHD010001625         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_176948_length_1170_cov_2.902242, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001625 DAORHD010000000
VERSION     DAORHD010001625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176948_length_1170_cov_2.902242"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..>1170)
                     /locus_tag="PKO06_24020"
     CDS             complement(196..>1170)
                     /locus_tag="PKO06_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HNV72796.1"
                     /translation="IPCEVKIKPVFATIIQDQRLPQFHISYAEGSVADKQVNEIPSAF
                     QPEFFNDDPHSDLLDREALLEGFRLTHCDAHVAFDDVNAELYQVDIEQVAQGDYSPQF
                     KKVDPIRRKRFMDLVRSLEGRARISALGKRLVELIGPRPEVSERELLAYVCRILDGME
                     PEKLDDCIERSEQYARRIKDKIDLLETDYRMKKFEEFLMTERVFLKPTFSFPKAIQPA
                     GVRSSGITGCLYMEEGDMNGFEQKFINEVANLPTVHFWHRNIERKGFRINGFINHYPD
                     FLVWTKSGRIVVVETKGDDRDNTDSQRKLKLGRAWADKAGSGYRLRHM"
BASE COUNT          294 a          318 c          269 g          289 t
ORIGIN      
        1 aaaacagtgg aagatttaat tgctttttca aaaagtgata atgtaataaa tagtcataaa
       61 aaagagagta aaaatgttta gtcaagaaaa ctatatagaa gttttaaatt ttgcagcaca
      121 tgctcatggt gaacaaaaaa caccgggcgt cgagaggcca cctcagaccg ggcttcctca
      181 ctatctcgcc gctcgctaca tgtgccgcaa ccgataaccc gatcccgcct tatcagccca
      241 agcccggccc agcttcagtt ttcgctggga gtccgtgtta tctcgatcat cccctttggt
      301 ttcgaccacg acgatccggc ccgattttgt ccacaccaga aaatccggat aatgattgat
      361 gaacccgttg attcggaacc cttttcgctc aatgttccga tgccagaaat gaaccgtcgg
      421 aaggttggca acctcattga tgaacttctg ctcaaaaccg ttcatgtccc cttcttccat
      481 gtaaagacag ccggttatgc ccgacgatcg cacccccgcg ggttgaatgg ctttgggaaa
      541 cgaaaaggtc ggcttgagga agacacgctc ggtcatcagg aactcctcaa acttcttcat
      601 gcgatagtcc gtctcaagca gatcgatctt gtccttgatt cgcctggcat actgttctga
      661 acgctcgatg caatcgtcca gtttttcggg ttccatcccg tcgagaattc tgcacacata
      721 cgccagaagt tcccgctctg aaacctccgg cctcggtccg atgagttcga caagtctttt
      781 gccaagggca gaaatgcgcg ctcttccttc aagagagcgc acgagatcca tgaaacgctt
      841 tcgcctgatc gggtcgactt tcttgaactg cgggctgtag tctccctgtg cgacttgctc
      901 aatgtccacc tggtacaact cagcgttcac gtcatcaaaa gccacgtgtg catcgcagtg
      961 cgtgagtcga aagccttcca gcagggcttc tcgatcaagg aggtcggagt gcggatcatc
     1021 attgaagaac tccggctgaa aagcgctcgg tatttcattc acctgtttat ccgcaacgga
     1081 accctctgca tacgagatat gaaactgcgg caaccgctgg tcctgaatga tcgtggcaaa
     1141 gaccggtttg atcttcacct cacagggaat
//
LOCUS       DAORHD010001626         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_177290_length_1169_cov_2.328546, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001626 DAORHD010000000
VERSION     DAORHD010001626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177290_length_1169_cov_2.328546"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1169)
                     /locus_tag="PKO06_24025"
     CDS             complement(<1..>1169)
                     /locus_tag="PKO06_24025"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV72797.1"
                     /translation="NGVSDGSSSSPWSSAWISWLTLLVAGLSLYKLWRQDPRALSGFI
                     QLLGAWWLQALLAIPLFPSSAPMLSGLFQGVLGAWSLLIRGLLLLTTALVIRSHAPDL
                     FATSPDLKSRLTRARHRFAGLLTFILISATIGAFLVNRTQLQRCLQDGTRDTLLIAGY
                     TQQLIEQTQAGATLLSTSPALQALFDDHASDQLKLTNVALDRYKRTYRDSLVYVMNAS
                     GTVLASSNRAERNSLVGRNFEFRPYFFEAMKGTETLYFALGKITMERGLYCSAPVFNA
                     SNTVILGVATIKISLNHLDHRFTMAAPTFLVDEKGIVLSGNRPELFLRPLWPQTADQL
                     NSYVNSNQYGDLNGTPILEQAPTIGRELAFDGNRALYVSTVLPGSTWSLVQLTPT"
BASE COUNT          277 a          285 c          388 g          219 t
ORIGIN      
        1 aacgtgggag tgagctggac cagagaccac gtgcttcccg gcagaaccgt tgacacgtag
       61 agagcccggt tgccgtcgaa ggccagttcc cggccaatgg ttggggcttg ttcgagtatc
      121 ggcgtgccgt tcagatcacc gtactggttg ctgttgacgt aggaattgag ttgatcggca
      181 gtctgtggcc agagtgggcg aaggaagagc tcgggacggt tccccgacaa cacgattccc
      241 ttttcgtcga ccagaaacgt gggtgctgcc atggtgaaac ggtggtcgag atgattcaga
      301 gagattttga tggtcgccac gccgagaatg accgtatttg acgcgttgaa gactggggcg
      361 gaacagtaca agccgcgttc catggtgatc ttgcccaggg caaagtagag cgtttccgtg
      421 cccttcatcg cctcgaaaaa atagggccga aactcgaagt tgcgcccgac cagactgttt
      481 cgctcggcgc ggttcgaaga ggccaggacc gtgcctgaag cgttcatgac atagaccaac
      541 gaatcacgat aggtgcgttt gtaccggtcg agagcgacat ttgtcagctt caattgatcg
      601 gaagcatggt cgtcgaagag agcttggagg gcaggcgaag ttgataacaa ggttgcgccg
      661 gcctgggtct gttcgatgag ttgctgggta tacccggcga tgagaagggt gtcgcgggtt
      721 ccatcctgca gacagcgctg cagctgggtg cggttgacca gaaacgctcc aatggttgca
      781 gaaatgagaa tgaaagtcag aagcccggca aaacggtggc gggctctggt cagacgactc
      841 ttcagatccg gtgaggtggc gaaaaggtca ggtgcgtgac ttctgatgac cagggctgtc
      901 gtcagcagca gaaggccacg aataagcagg gaccaggcac caagcactcc ctggaacaga
      961 ccggaaagca taggcgcgga agatgggaag agcgggattg cgagcagggc ttgcaaccac
     1021 caggcaccca ggagctggat gaaaccagac aaggctcggg ggtcttgacg ccagagcttg
     1081 tagagagaaa gcccggcgac cagaagtgtg agccaggaga tccaggcgga actccagggg
     1141 ctggaactgc tcccatcact cactccgtt
//
LOCUS       DAORHD010001627         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_178883_length_1163_cov_2.147112, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001627 DAORHD010000000
VERSION     DAORHD010001627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_178883_length_1163_cov_2.147112"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PKO06_24030"
     CDS             <1..841
                     /locus_tag="PKO06_24030"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNV72798.1"
                     /translation="SRGAGKNPGWIESYLKEPETFADRVTAGDSHDVNPARVLWRGIL
                     PGLLDMPDHEVNTFINGYLQTYVERDVRVLGEIRELGAFGTFIGILGGLTAQEINHSL
                     LGREVGISPVTARKWLDLLMATFQWHELPGFSGNVLKRVSGKRKGHFADTGLACALQR
                     LGSPEAVPVARFAGSLFESFVVNDIRGQLAALPSLPDLYHWRTSGGAEVDLILDYNGM
                     LFPIEMKFTTRLSGNDQRGIRAFRDTYPRRNVAPGLIVYVGSEVRRVSDVATAIPWTA
                     TIG"
BASE COUNT          229 a          304 c          344 g          286 t
ORIGIN      
        1 gtctcgaggg gccggcaaga atcccgggtg gattgagtcc tatctgaaag agccggaaac
       61 ttttgctgat cgggtgacgg ctggcgattc gcacgacgta aaccctgcgc gtgtcctgtg
      121 gcgcggcata ctccccggac tgctcgacat gcctgaccat gaggtgaaca cgttcatcaa
      181 cgggtatctg caaacgtatg ttgagcgtga cgtcagagta ctgggggaga ttcgtgaact
      241 gggggccttt gggacattca tcggcattct tggggggctc accgcacagg agatcaacca
      301 ctctctcctt ggtcgggaag ttggcattag cccggtcacg gctcgaaagt ggctcgatct
      361 cctcatggct acgtttcaat ggcatgagct tccgggtttt tctggaaacg tcttgaagcg
      421 tgtctcagga aagcgcaaag ggcatttcgc tgatacaggg ctggcctgtg ctcttcagag
      481 actgggttct ccggaggctg ttcccgttgc tcgatttgcc gggtcattgt tcgaatcgtt
      541 cgtggtgaat gacatccgag gacagttggc ggctcttcct tcccttccgg atttgtatca
      601 ctggcgcacg agtggaggcg cggaagtcga tctgattctc gactacaacg gcatgctgtt
      661 ccccatcgaa atgaagttta ccacccggtt gtcaggaaat gatcagcggg gtattcgtgc
      721 atttcgcgac acctaccccc ggcgtaacgt tgctcctggc ctgatagtct acgttggctc
      781 ggaagttcgc cgggtttcag acgtcgccac agccattccc tggacggcca ccatcggata
      841 gactcatggg ttgcaccagc tccgcagcca tggcgcatga gcgattgtga ccatggaata
      901 tggcggaaag aaaccatttg aagtagggcc gggacttcgg tatcatgcaa cggttctcaa
      961 tagtacggtt ctctaaaact gaaggaggat gtttatgtct atcaaaaaga tgcttctcgc
     1021 gattgtttgt tgtgtctctc tcctcgccgt cggctgcggc ggcggtggcg gcggcggtgg
     1081 cgctacaacc ccgaatcagg tgacgttaac aacgaatgtt gttgttccgc cccctgtcgc
     1141 cccggaaacc agtattcgtg cat
//
LOCUS       DAORHD010001628         1162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179059_length_1162_cov_3.064137, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001628 DAORHD010000000
VERSION     DAORHD010001628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179059_length_1162_cov_3.064137"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1162
                     /locus_tag="PKO06_24035"
     CDS             <1..>1162
                     /locus_tag="PKO06_24035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72799.1"
                     /translation="RARQQETIFRTLFETNPSSITIQRASDAGFLMVNPAFQHQIGLP
                     LDQILGRTGEELGIKIAADSANTIQTQLQSTGRVDNILAHTRLPNGQEMVSLFSSRHI
                     DFDGQPCILSVALDVTEVKRLQEQMHHVQKLDAIGQLAGGVAHDFNNMLTGILGSAEC
                     LSLELDKNQTGREYLDMIIEATRRAADLTRNLLTFARKNRLEMTEIDVHLVITNSVKL
                     LQRTLNKSVKIETSLDNRNPKIRGDASQLMNALINLGINAGHAMPDGGTLKFSTREVM
                     LDEVFCQASSFDLRPGIYIVVSIEDSGTGISPEHLPHIFEPFFTTKEKDKGTGLGLAA
                     VFGTVCQHRGAITVYSEPGNGTIFHVYLPSFGAAHDTDAKPDRDAEAVAFRGS"
BASE COUNT          267 a          361 c          313 g          221 t
ORIGIN      
        1 cgggcgcgac agcaggaaac cattttccga acgctgttcg agaccaaccc ctcgagcatc
       61 acgattcaac gagccagcga tgccggcttc ctcatggtca acccggcgtt tcagcatcag
      121 atcggcctgc ccctggatca gattctcggt cgcacgggcg aagaactggg tatcaagatc
      181 gccgccgact cggcgaatac cattcaaacc cagttgcagt cgaccggtcg cgtcgacaac
      241 attctggcac acaccaggct gccgaacggt caggaaatgg tcagcctctt ctcttcacgt
      301 cacattgact tcgatggcca gccctgcatt ctgagcgttg ctctcgatgt caccgaggtc
      361 aagcgactgc aggagcagat gcatcacgtg cagaagctcg acgcgatcgg acaactggcg
      421 ggtggggtcg cgcacgattt caacaacatg ctgaccggca ttctcggatc agccgagtgc
      481 ctgagcctgg aactcgataa aaatcagacc ggtcgcgaat atctcgacat gatcatcgaa
      541 gccactcgcc gtgcggccga tttgacacgc aatctgctca ccttcgcccg caagaatcgt
      601 ctcgagatga ccgaaatcga cgtgcatctg gtgatcacca attcagtcaa gttactgcag
      661 cgaacactga acaagagtgt caagatcgaa accagtctgg acaatcgcaa cccgaagatt
      721 cggggagacg cctcgcagct catgaatgcc ctgatcaatc tcggcatcaa cgcgggtcac
      781 gccatgcccg acgggggaac gctcaagttc tcgacgcgag aggtcatgct ggacgaagtg
      841 ttctgtcagg catcgtcatt cgatctgcgc cccggcatct acatcgtcgt ctcgatcgaa
      901 gacagcggca ccgggatttc gcccgaacat ctcccgcaca tcttcgaacc ctttttcacc
      961 accaaggaga aggacaaagg caccggtctt ggtctggcgg cggtctttgg caccgtctgc
     1021 cagcaccgtg gcgcgatcac ggtgtatagc gaaccgggca atggcaccat ctttcatgtg
     1081 tatcttccgt cattcggagc cgcccacgac acggatgcca agcccgacag ggacgcggaa
     1141 gccgtcgcat tccgcgggag cg
//
LOCUS       DAORHD010001629         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179260_length_1161_cov_4.058770, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001629 DAORHD010000000
VERSION     DAORHD010001629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179260_length_1161_cov_4.058770"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKO06_24040"
     CDS             <1..273
                     /locus_tag="PKO06_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72800.1"
                     /translation="VKYFNALPVLEDPQTGGQLDWGYGSPSAYFEDFDSDGRFDIDTE
                     FAYTGASNAYASSTDRVISRALSATGSTFIPLDYVDVTVTTSEGPM"
     gene            354..>1161
                     /locus_tag="PKO06_24045"
     CDS             354..>1161
                     /locus_tag="PKO06_24045"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV72801.1"
                     /translation="MSAEFLKRKRIGEILIEMNAITPSQLELALKRQPSARKMIGEIL
                     IEDKAVTEETLFRGLSKQHGIEFVNLNGAELNRELIRQIPESLIKTYKFIPLKKEPKS
                     LTIAVFDPSNTKMFDSIKLATGYMFIKWVLAMKSQIISIINENIYRPQNVLENVPVED
                     LLRDVKLEFSKDETSVMTGTSGTTSEVNLEELKRESDQTSIVTLVNKIILDAVKMRAS
                     DIHIEAFENIIQIRYRVDGILYDMMPIEKAAHQAIISRLKIMANLDITERI"
BASE COUNT          308 a          294 c          322 g          237 t
ORIGIN      
        1 gtgaaatact ttaacgcgct gccggttctt gaagacccac agaccggagg tcaattggac
       61 tggggatacg gcagtccatc ggcgtatttc gaagacttcg attctgacgg acgtttcgat
      121 atcgataccg agttcgcgta taccggagcc tcaaacgcgt atgcttcgag cactgatcgc
      181 gtgatctctc gggcgttgtc ggcgacgggc agtaccttta tcccgctcga ttacgtcgat
      241 gtgaccgtga caacgagtga ggggccgatg tgaggtgagc cgggaacggt tcggtgaaag
      301 ctcaactttc gcgggtttgc ggtcgataac acagtgaaag gataccctga accatgtctg
      361 ctgagtttct gaaacgaaaa cggatcggtg agattctcat cgagatgaat gccatcacgc
      421 catcgcaact cgagttggcg ttgaagcggc aacccagcgc ccgcaagatg atcggggaaa
      481 tcctcatcga ggacaaggcg gtcacggaag agacgttgtt tcgcggtctg tcgaaacagc
      541 atgggatcga gttcgtgaat ctcaacggcg cagaactgaa tcgtgaactg attcggcaga
      601 ttccggaaag cctgatcaaa acgtacaagt tcattccgct gaagaaagag ccgaaatcgc
      661 tgacgatcgc ggtctttgac ccaagtaata ccaagatgtt cgacagcatc aagctggcaa
      721 ccggatacat gttcatcaag tgggtgctgg cgatgaagag tcagatcatc agcatcatca
      781 acgagaacat ctatcgacca cagaacgtgc tcgagaacgt accggtcgaa gacctgctgc
      841 gcgacgtcaa gctcgaattc agcaaggacg agacgtcggt catgacgggg accagtggca
      901 cgaccagcga agtgaacctg gaagaactca agcgcgagtc ggaccagacc tcgatcgtca
      961 cgctggtgaa caagatcatt ctcgacgcgg tcaaaatgcg cgcgtcagac attcatatcg
     1021 aagccttcga aaatatcatt cagatccgct atcgcgttga cggtatcttg tacgacatga
     1081 tgccgatcga gaaagcggca catcaggcga tcatttcgcg cctcaagatc atggcgaacc
     1141 tcgatatcac cgaacgcatc a
//
LOCUS       DAORHD010001630         1161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179284_length_1161_cov_3.254973, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001630 DAORHD010000000
VERSION     DAORHD010001630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179284_length_1161_cov_3.254973"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PKO06_24050"
     CDS             complement(<1..1072)
                     /locus_tag="PKO06_24050"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72802.1"
                     /translation="MRLVQHGVAQTRAGTMQVDSPSSGVVRRLRHLPYVAVCLSQTRD
                     SPALALTTRWLPMCAAFAWLAGIVWLASRFLARGFLLPMHRLAHAGSEVARGEYRITL
                     DEEQSGREFQAISRAFNRMTHGLREGRLLSRFVSSQATSTSDQTEEAKVESLSLGGDR
                     RTVIVMFSHVADFVRVTAHLNPHQIMEMLNQYFSRMEPLIERFGGRIDKYIGDAIMAV
                     FPTDESQESSLHAIQAALAMRQALAEFNRSSDESWPRLTTGIGMAEGEVIAGRVGSRT
                     RRLDYTVIGDVVNLAARLEALRERLPAGAVIINETLATRVSHQIKVERSGYLDIKGKV
                     QRVPVFFVPEPELGFALAAIAPL"
BASE COUNT          271 a          363 c          309 g          218 t
ORIGIN      
        1 ccagcggcgc gatcgccgcg agcgcgaagc cgagctccgg ctccggaacg aagaatactg
       61 gcacccgttg tacttttccc ttgatgtcca gatatccaga tcgttccacc ttgatctgat
      121 gagatacacg agtcgcaagc gtttcattga tgatgacggc accagcaggc agacgctcgc
      181 gcaacgcctc gagacgggca gccagattca cgacgtcgcc aatgacggtg tagtcgagtc
      241 ggcgagtgcg cgaaccgact cgaccggcaa tgacttctcc ctctgccatc ccgattccgg
      301 ttgtcaggcg aggccagctt tcgtccgagc tgcgattgaa ttccgcgagt gcctgacgca
      361 tggcaagagc tgcctgaatg gcgtgaagag aactttcctg agactcgtct gtcggaaaca
      421 cggccataat ggcatcgccg atatacttgt caatgcgacc gccgaatctc tcgatcagcg
      481 gctccattcg cgagaaatac tgattcagca tttccatgat ctgatgagga ttcaaatgtg
      541 cggtaacccg gacaaaatcc gcaacatgtg aaaacatcac gatcacggta cgccggtctc
      601 cgccgagaga gagactctca accttcgctt cttccgtctg gtcagaagtc gaagttgcct
      661 gtgatgacac aaaccgcgac agcagacgcc cttcccgcag cccatgcgtc atgcgattga
      721 aagcacgtga gatcgcctga aattctcttc cgctctgctc ttcatcaaga gttatccggt
      781 actctccacg agcgacttca ctgccagcat gggccagacg atgcatcggc aacagaaatc
      841 cccgggcaag aaatcgcgat gccagccaga caataccggc cagccaggca aatgcagcac
      901 acatcggaag ccaccgggtg gtcagggcca gcgccgggga atcccttgtc tgcgacaggc
      961 agacggccac gtatggcaga tgccgcagac ggcgaaccac cccactcgat ggcgagtcaa
     1021 cctgcatcgt accagcgcgt gtctgggcaa ctccgtgctg taccaggcgc accacctcat
     1081 gatttctgtc gattccaggc ggccagcaac ggacgagttg tcctccctcc ggctcgacac
     1141 gaaagaccgc catcaaccag g
//
LOCUS       DAORHD010001631         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179486_length_1160_cov_4.168326, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001631 DAORHD010000000
VERSION     DAORHD010001631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179486_length_1160_cov_4.168326"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKO06_24055"
     CDS             complement(<1..367)
                     /locus_tag="PKO06_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72803.1"
                     /translation="MNQVLGVIGLCRRRDERLELRCRQLSGIRWVEIKFDVSAGWTGN
                     KNQPVWDAGDAPQCLTDLLKMLIGTQRPRVSRPGGWMFMITKGTDDVMNLSVEPDMTL
                     KVGLKSACFGEEFAPQNADN"
     gene            467..>1160
                     /locus_tag="PKO06_24060"
     CDS             467..>1160
                     /locus_tag="PKO06_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72804.1"
                     /translation="MRPITSVHHRFGSGYVGTWHKPSPSRDPLAKTPGALLALYHINE
                     LPLRAIERFARRRANPGFKREIRFEPIAPLPRFLSGEHDLTLVTSLVDTDFAQQYHRT
                     HRAKMLQGLQPVLIVHGKKHRSAHTSSWVGSFIPLGYLTLVCSLLLLWRMSWLDLLGT
                     WNLTSRLRTGFALCLIPVIGMLVFASASWLQLQRTNEQVEIRRFLTTHLDHLNQTIHF
                     FIDKFAQVLSHRS"
BASE COUNT          228 a          385 c          273 g          274 t
ORIGIN      
        1 tgttgtcagc attctgcggg gcgaactctt cgccgaagca cgcgcttttc aatccgacct
       61 tcagtgtcat gtctggctca acgctcaaat tcatcacgtc atccgtgcct ttggttatca
      121 tgaacatcca accgccaggg cgagagacac gtggtctctg ggtgccgatc agcattttca
      181 acagatctgt caggcactga ggcgcgtcac cggcgtccca cactggctga ttcttgtttc
      241 cggtccagcc ggcagagaca tcaaacttga tctcaaccca gcgtatgccg gaaagctgcc
      301 gacatcggag ttccagtctc tcatcgcgtc ggcggcacag gcctattact cccagcacct
      361 gattcatcaa agcactccga atgatctgga ccttgtgacc cgaatcaact tgagattgaa
      421 ccccctgatc ggttgcaacg cctttctttt tcaatccctc tccttcatgc ggcctatcac
      481 ctcggtccat catcggtttg gcagcggcta tgtcggaacc tggcacaagc cctcccccag
      541 ccgagaccca ctggcgaaaa cacccggggc cctgctcgct ctctaccaca tcaacgagtt
      601 acccctgcgc gcgatcgaac gcttcgccag gcgacgagcg aaccccggtt tcaagcgtga
      661 gatccgtttc gaacccattg ctcctctgcc ccgctttctc tctggcgaac acgacctgac
      721 tctggtgacc agcctggtcg ataccgactt tgcacagcag tatcaccgca ctcatcgggc
      781 aaagatgctt cagggtctcc aaccagttct gatcgtgcac ggcaagaaac atcgctctgc
      841 tcacacctca tcctgggtgg gctcattcat tcccctcggg tatctgaccc tcgtttgcag
      901 cctcctgctg ttgtggcgca tgtcgtggct tgacctcctc ggcacatgga atctcacctc
      961 acgcctccgc accggattcg ctctctgtct cattccggtc atcgggatgc tggtgttcgc
     1021 ctctgcgagc tggctgcagc tgcaacggac aaacgagcag gtcgagattc ggcgatttct
     1081 gaccacccat ctcgatcacc tcaatcagac aattcacttt ttcatcgaca agttcgcgca
     1141 agtgctgagt caccggagtg
//
LOCUS       DAORHD010001632         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179746_length_1159_cov_4.183877, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001632 DAORHD010000000
VERSION     DAORHD010001632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179746_length_1159_cov_4.183877"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKO06_24065"
     CDS             <1..960
                     /locus_tag="PKO06_24065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV72805.1"
                     /translation="QAGTNDLKTVICLTDGRDETPGGKSPFSSKKPEELIEFARRARV
                     RLITVGLGNGIDTNLLQSCATRTNGWYLHVPSPGQLAKAYQELSRKMTMERVYHFRYT
                     TPEAKPDGTRRDLTVNSKAKGEKDQGKGYYIAPTVEQLKSLQERAAGSTGTSGGKTNV
                     KLNPPDLGKPGSVGRLGPADLGTTGQVGHIGSQSVGSLGQIGSLGPGLEHVETEVNQM
                     FKQLDQQNQQHLDNGINQGNQAIDTANESNAAIDAANQQQLDQTISDTNTSLEQTDQT
                     NQDAAQDAQDAVNQSYDQMNQQMQDAMPDVPSMPDMPDTSGDD"
BASE COUNT          281 a          381 c          323 g          174 t
ORIGIN      
        1 caggccggca cgaacgacct gaaaaccgtg atctgtctca ccgacggtcg tgacgagact
       61 ccgggcggca aatcgccgtt ttcctcgaag aaaccggaag aactgatcga gttcgcccga
      121 cgcgcccgcg tgcgcctgat caccgtcggg ctcggcaacg gcatcgacac caacctgctt
      181 caatcgtgcg cgaccagaac gaacggctgg tacctgcatg ttcccagccc cgggcagttg
      241 gccaaagcgt accaggaact cagccggaag atgacgatgg aacgcgtgta tcacttcagg
      301 tatacgacgc ccgaagcaaa gcccgacggc acgcgccgcg acctcaccgt gaacagcaag
      361 gcgaagggtg aaaaggatca ggggaagggc tactacatcg ccccgaccgt cgaacagctc
      421 aagagcctgc aggagcgggc ggcgggtagc accggcacga gcggcggcaa gaccaacgtg
      481 aaactgaacc cgcctgatct cggcaagccc ggcagtgtcg gtcgccttgg ccctgccgac
      541 ctcggcacca ccggacaagt cggtcacatc gggtctcaga gcgtcggctc actcggacaa
      601 atcggctcgc tcgggcccgg gctcgagcac gtcgaaaccg aagtgaatca gatgttcaaa
      661 cagctcgacc aacagaatca gcagcatctc gacaacggca tcaaccaggg caaccaggcc
      721 atcgacacgg cgaacgagag caatgccgcc atcgatgcgg cgaaccagca acagctcgac
      781 cagacgatct ccgacaccaa cacttccctc gagcaaaccg accagaccaa ccaggatgcc
      841 gctcaagatg cgcaggacgc ggtcaaccag tcttacgacc agatgaacca gcagatgcag
      901 gacgcgatgc ccgatgtgcc cagcatgcct gacatgccag acacctcagg cgacgactga
      961 cccagacacc accctctctc gcgaccgtta tcttctgcag tgggagaaga tcccgaacaa
     1021 gggccgttac cttgcctgta cggtctgctg tatctgcgtc gaactccatc aacgattcgg
     1081 attttctgaa accctgtcct tgagaatcgc cagatagcct ggtgttttcc gaggtcgccc
     1141 ttgtcgccaa tcgtcgggt
//
LOCUS       DAORHD010001633         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179772_length_1159_cov_3.418478, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001633 DAORHD010000000
VERSION     DAORHD010001633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179772_length_1159_cov_3.418478"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1159
                     /locus_tag="PKO06_24070"
     CDS             <1..>1159
                     /locus_tag="PKO06_24070"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72806.1"
                     /translation="LLAAAEAVLDPEQALFDYAQFPANQLDRLWLFGFDDDRDDPVLE
                     ALFVAFDAISRFKKNLESQPQLAQARARLSIGLALGPVTRFIRGPLGHTTLAGKTVYL
                     AETLAEAAGDFQIYIDQEIHHLSQPLFDFREWKPMKLRPILPQLSFFEVVGLNKREEI
                     FAFASHKEAVFRQAVAISYRYLEMSDVTPLLSLVADSDERVALEALKTVSFLGDQRAQ
                     GILKKYLSETKNAGLRAAVIEALSATGKEDIVPLLLAASKDVHWQVRFKAAQGLYTIL
                     RQDSIKHLAPLRQDDDGAVRSAIHEIYFKETGERSEVQALGNLLTDLSVRARKAAMEA
                     LARIGNDDAMQLLAQSFRQQDHDLRKAMVRVVAGSRSPQSHACLLKMFANCD"
BASE COUNT          227 a          374 c          333 g          225 t
ORIGIN      
        1 gctgctcgcc gccgccgaag ccgttctcga cccagagcag gccctcttcg attacgcgca
       61 gtttcccgcg aatcaactcg accgcctgtg gctgttcggg ttcgacgatg accgtgatga
      121 tccggttctg gaagccctct tcgtcgcctt cgacgccatc agtcgtttca agaagaacct
      181 cgaaagtcag ccccagctcg cgcaggcccg agcccgtctt tccatcggtc tggcactcgg
      241 tccagtcacg cgtttcattc gcgggccact cggacacacg accctcgccg gcaagacggt
      301 ctatctggct gagacgctgg ccgaggccgc aggagacttt cagatttaca ttgatcagga
      361 aattcaccac ctgtcgcagc ccctgtttga cttccgcgaa tggaagccga tgaaactgcg
      421 gccgattctg ccgcaactgt cgttcttcga agtcgtcggt ctgaacaagc gcgaagagat
      481 cttcgccttt gcttcgcaca aggaagccgt gttccgccag gctgtcgcca tctcctaccg
      541 ctatctggaa atgagcgacg tcacccccct gctgagtctg gtcgccgact cggacgagcg
      601 ggtcgccctc gaggccctga aaacggtgtc attcctcgga gatcagcggg cgcagggcat
      661 tctaaagaag tatctcagcg agacgaagaa tgccggcctg cgtgccgcgg tcatcgaggc
      721 cctcagcgcg accggcaaag aagacatcgt gccgcttctg ctcgccgctt ccaaggacgt
      781 gcactggcag gttcgcttca aggccgccca agggttgtat accattctcc gccaggacag
      841 catcaagcat ctggcgccgc tgcggcagga cgatgatggg gcggtgcgat cagccattca
      901 cgagatctat ttcaaggaaa ccggtgagcg cagcgaagta caggctctgg gcaatctgct
      961 gaccgatctg tcggtgcgcg cccgcaaagc cgccatggaa gcgctggcgc gcatcggcaa
     1021 cgacgatgcc atgcaactgc tggcccagtc tttccgacaa caggaccacg acctgcgcaa
     1081 agccatggta cgagtggtcg cgggctctcg ttcgcctcag agccacgcct gcctgctgaa
     1141 gatgttcgcg aactgtgac
//
LOCUS       DAORHD010001634         1159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_179823_length_1159_cov_2.762681, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001634 DAORHD010000000
VERSION     DAORHD010001634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_179823_length_1159_cov_2.762681"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKO06_24075"
     CDS             complement(<1..836)
                     /locus_tag="PKO06_24075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72807.1"
                     /translation="MKRIVFVSILLLALAVTVQAQSNPYADQEITGTQGKSDRIAAVP
                     SSTAVATTDTAATALPKTVLDKLVLPIQENGKVRLLKGAAAWAYLQQKNPKLAQALQK
                     LCNSVSSRPDLNAKLTDLLQRVTSLQITDGTIVLSLPGSRFLVIDRDGLDRVLPFSYA
                     ALDADEKRILDNSRIPEMIGPEGQPLQGRALFEELARRNPKALLAFTNLCARLRTPEY
                     TVTTPEGSKSALSMIQGLFYQERDRFRAFCDQRLMQLIASNRQFSKAPGHGDHFPTSF
                     KQ"
BASE COUNT          245 a          295 c          360 g          259 t
ORIGIN      
        1 ttctgtttga aactcgtggg aaagtggtcg ccgtgtccgg gagccttgct gaactgtcga
       61 ttgctcgcaa tcagttgcat caagcgctgg tcgcagaaag cgcgaaagcg atcgcgctcc
      121 tgataaaaca acccctgaat catgctgagc gcggatttcg acccttcggg cgtggtgacg
      181 gtgtattcgg gagtgcgcag tcgggcacag aggttcgtga aggccagcag tgctttcggg
      241 ttccggcggg ccagttcctc gaacagcgct cttccctgca gtggttgtcc ttcaggcccg
      301 atcatttccg gaatccggga attatcgagt atccgttttt cgtcagcatc aagggcggca
      361 taggaaaacg ggagcacgcg gtcgagaccg tcgcggtcga tgaccaggaa ccgtgacccc
      421 ggcaacgaaa gaacaatcgt gccatccgtg atctgaaggc tggtgacgcg ttgcagcagg
      481 tccgtgagtt tcgcgttgag atccggccgg ctcgatacgg agttgcagag cttctgcagg
      541 gcctgagcca gcttcgggtt cttctgctgc agataggccc acgcggccgc gcccttgagc
      601 agtcgcacct tgccgttttc ctgaatcggc agcacgagct tgtcgagcac ggttttcggc
      661 aatgctgtcg ccgctgtgtc ggtagtcgca accgctgtgc ttgagggcac tgcggcaatc
      721 cgatcgcttt ttccctgcgt gccggtgatt tcttgatcgg cgtaggggtt cgactgcgcc
      781 tgaactgtga ccgccagggc gagcaacaag atgctgacga agacgattcg tttcataggc
      841 ttcctcagaa taggatatgt tcgattatag tgaagaattc aggttatggc cagtcgtggc
      901 gagaaattga gcctgcccaa tcacgatcga ggaagaggga attccggaaa acagccgtct
      961 gaaagggcgg gagcggaagt cgcctgtatc ttttccgccc gtgatgcggt ataatgacgc
     1021 aaaatcaaca ggggtgggct ggggtatgaa aatgcgtatt ctgcacatcg gtgacgacgt
     1081 attacgcgct ccgtcagagc cggtgaaaga aatcaacgac gatctgcgca aactggtcaa
     1141 ggatatgttt gaaaccatg
//
LOCUS       DAORHD010001635         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_180297_length_1157_cov_3.622505, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001635 DAORHD010000000
VERSION     DAORHD010001635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180297_length_1157_cov_3.622505"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKO06_24080"
     CDS             complement(<1..763)
                     /locus_tag="PKO06_24080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV72808.1"
                     /translation="MKRRQSITVGIGGMILLVFVLFFGTQARVAAAATGWEKEVLYFV
                     LLDRFENGTTMNDFDVRRRDPRAFHGGDLHGLIQRLDYLQDLGVTAIWLSPIYQNRPM
                     PFYGQGAYHGYWPWDFFAVDARFGNDFKVMELRQELRKRNMKLVLDLVVNHAGYDAPL
                     VQTHPILFHANPEISDWNDRNQVENFGVFGLPDFASERAVVQRFHQAVSSHWLELAEP
                     DGYRLDAVKHVPLSFWKTFNRRLADTQGDDFLLLGE"
     gene            complement(807..>1157)
                     /locus_tag="PKO06_24085"
     CDS             complement(807..>1157)
                     /locus_tag="PKO06_24085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV72809.1"
                     /translation="AFLCLRLELKNQDGTAIALDSLREQLMGEIGRVLLLHQRPNDLS
                     ARFSTDKLIVLLYEATKEGARAFHRRIQKDVEKSLATFQEGRDLRATLHIAVLSFPED
                     TLEREGMLALIEKS"
BASE COUNT          245 a          357 c          311 g          244 t
ORIGIN      
        1 gttcgcccag cagcagaaag tcgtccccct gcgtgtcggc caaccgtcgg ttaaatgtct
       61 tccaaaacga gagaggcaca tgttttaccg cgtcgaggcg atatccatca ggctcagcca
      121 gttcgagcca atgggagctg accgcctgat gaaaacgctg cactacggca cgctctgagg
      181 cgaaatccgg cagaccaaag acaccgaagt tctcgacctg attccggtcg ttccagtcag
      241 aaatctcggg gttggcatgg aacaggatgg gatgcgtctg aaccagcggc gcatcgtatc
      301 ccgcgtggtt caccaccaga tcgagtacga gtttcatatt tcgctttcgc agttcctgcc
      361 tcagttccat taccttgaaa tcgttgccga aacgcgcatc gaccgcgaag aaatcccagg
      421 gccagtagcc atgatacgcc ccctgcccgt agaacggcat ggggcgattc tgatagatcg
      481 gactcagcca gatcgcggtc actccaagat cctgcaggta gtcgagacgt tggatcagtc
      541 cgtgcaggtc tccgccgtga aaagcgcgtg gatctcggcg tctgacatcg aagtcgttca
      601 tcgtggtgcc gttctcgaac cggtcgagca gcacgaagta caacacctct ttctcccacc
      661 cggtagccgc ggctgcgact cgtgcctgcg tgccgaagaa cagtacgaaa accaggagta
      721 tcatccctcc gattcccacc gtgattgatt gtcgtcgttt cattcccctg ccctgccttt
      781 ccgtgctctt tccgctcgcg gcggcgtcac gatttttcga tcagtgccag catcccttcc
      841 cgttcgagcg tgtcttccgg aaaggagagc acggcaatat gcaacgtggc ccgcagatcg
      901 cgcccttcct gaaacgtcgc cagcgacttc tcgacatctt tctgaatgcg ccggtgaaac
      961 gcgcgagccc cttccttggt cgcttcgtag agaagtacga tgagcttgtc ggtagaaaaa
     1021 cgggccgaca gatcgttcgg gcgctgatgc agcagcaaca cgcgaccgat ttctcccatc
     1081 agctgctccc gcagactgtc gagcgcaatc gccgtgccgt cctggttctt cagctcaaga
     1141 cgcagacaga gaaaggc
//
LOCUS       DAORHD010001636         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_180501_length_1157_cov_1.562613, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001636 DAORHD010000000
VERSION     DAORHD010001636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180501_length_1157_cov_1.562613"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PKO06_24090"
     CDS             complement(<1..425)
                     /locus_tag="PKO06_24090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72810.1"
                     /translation="MLILHHRNGFVGFMLTMALGVALGAATLVVSNEVIKGAKKEGTK
                     PATSQSSPFKDRANALKVAWKNYDQTRQAYEDALAQRSPDVATKRETMLRAKRILERA
                     ILGATPGLRARGRVPCGCGRAGTRLSSVGHGRGRKAGSGS"
     gene            676..>1157
                     /locus_tag="PKO06_24095"
     CDS             676..>1157
                     /locus_tag="PKO06_24095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HNV72811.1"
                     /translation="MKYRVIQVFIVSLLCFPLFVPATVSAAKPLVLFDEGHAQRAGNA
                     DWVIDGGFSDFADVFKSLGCEVRAVKQLTASHLVGASVIVLPEPNSVYTPEEEAAIVE
                     FVNGGGGLYAIADHDRSDRNGDGIDSVGVLNRFLPKLGLQLNKLYFTEAPDAGSYKET
                     P"
BASE COUNT          235 a          302 c          335 g          285 t
ORIGIN      
        1 gagccactcc cggccttcct gccgcgcccg tgaccgacgg aggacaggcg agttccggca
       61 cgaccgcaac cgcagggaac tctgcccctg gcacgaaggc cgggagtggc tccgaggatg
      121 gcgcgttcga gaattcgttt ggcgcgcagc atggtttcac gcttggtggc cacgtctggc
      181 gatcgctgcg ccagagcgtc ttcataggcc tgacgagtct ggtcatagtt tttccaggcg
      241 actttcagag cattggcccg gtctttgaat ggcgaagatt gagaggttgc cggtttcgtg
      301 ccctctttct tggccccctt gatgacttca ttgcttacga cgagtgtcgc ggctccgaga
      361 gcgacgccca gagccatggt cagcatgaat ccaacgaatc cgtttcggtg gtgaagaatc
      421 aacatgagcg cacctcgctt tccgggaaat cccggcgacc agatcaagta ttctcagtat
      481 acaaattttc ggcctccggc ggaaccgggt cagacaaaac ctgttttgtc catcgaaata
      541 caggttatgt tgtttgtgaa acgcacttga atgggaaaaa tcgtcattga cgcttggtgg
      601 tcatcactct atactgaata tatctggaga gccttctccg gacgtttttc catcctggga
      661 aaagaaggac cgagtatgaa gtatcgagta atccaggttt tcattgtcag tctgctttgt
      721 ttcccgttgt ttgtaccagc aactgtgtct gctgcgaagc ctctggtgct gttcgatgag
      781 gggcatgccc agcgtgccgg aaatgctgac tgggtcatcg atggagggtt ctctgacttc
      841 gccgatgttt tcaagagcct ggggtgtgag gtgcgggcgg tgaaacaact gacggcttct
      901 catcttgtgg gcgcttcggt cattgtgctc cctgaaccga acagtgtgta tactcccgaa
      961 gaagaagcgg ccattgtcga gtttgtcaac gggggtggcg gtttgtatgc catcgccgat
     1021 cacgatcgtt cagaccgcaa cggcgacgga atcgactcgg tcggcgtctt gaatcgcttt
     1081 ctgcctaaac tgggcttaca gttgaacaag ctgtatttca ccgaagcgcc ggacgccggc
     1141 tcatacaagg aaactcc
//
LOCUS       DAORHD010001637         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_181437_length_1153_cov_2.838798, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001637 DAORHD010000000
VERSION     DAORHD010001637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181437_length_1153_cov_2.838798"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1153
                     /gene="asnB"
                     /locus_tag="PKO06_24100"
     CDS             <1..>1153
                     /gene="asnB"
                     /locus_tag="PKO06_24100"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HNV72812.1"
                     /translation="ELRKQLQRPHPFQTESDCEVILYLYDEHGPEFLNRLNGIFAFIL
                     WDPKNNDYLIARDHIGIIPLYIGWDTDGNMYVASEMKAIADRCKRLEEFPPGHYYKGS
                     EGKMVKYYRPAWAEPNVVPQKPLDLGFLRQSLEEAVRRQLMCDVPYGVLISGGVDSSI
                     ISAVAARYCARRVESAGKEQAWWPTLHSFSVGLKESPDLKMARKVAEYIKSTHHEIHF
                     SVQEGLDALSDVIYHLETFDITTIRAATPMYLMARKIRAMGIKMILSGEGADEVFGGY
                     LYFHKAPNKVELHEETVRKLSMLHKYDCLRANKAMAAWGVEARVPFLDREFLDVAMTV
                     DPAEKMCGGGKIEKKMLREAFKGYIPDEILWRQKEQFSDGVGYSWIDTL"
BASE COUNT          263 a          312 c          333 g          245 t
ORIGIN      
        1 aggagctgcg gaaacagctg cagcggccgc acccgtttca gaccgaatcg gactgcgaag
       61 tcattctcta tttgtatgat gaacacgggc ccgagttcct caacagactg aacggcattt
      121 ttgccttcat cctctgggat ccgaaaaaca acgattatct cattgccaga gatcatatcg
      181 gcattattcc gctctacatc ggctgggaca ccgatgggaa catgtacgtt gcgtcagaaa
      241 tgaaggcgat tgccgatcgc tgcaagcgtc tcgaggagtt tccgcccggg cattactaca
      301 agggcagcga aggcaagatg gtcaaatact atcgtccggc atgggccgag ccgaatgtgg
      361 ttccccagaa gcctctcgac ctcggctttc tgcgacagag tcttgaggag gccgtgcgac
      421 gtcagctgat gtgcgatgtt ccatacggag ttcttatctc cggtggcgtc gattcctcga
      481 tcatttcggc ggttgcggcc cggtactgtg cccgccgtgt cgaatcggca ggcaaggaac
      541 aggcctggtg gccaaccctg cactcgtttt cggtcggtct gaaagagtcc cccgatctga
      601 agatggcgcg aaaggtcgcc gagtacatca aatcgacgca ccatgaaatt cacttctcag
      661 tccaggaagg cctcgacgcc ctcagcgatg tcatctatca tctcgaaacc ttcgatatca
      721 cgaccatccg ggcggcaact cccatgtatc tgatggcgcg caagattcgg gcgatgggaa
      781 tcaagatgat tctttccggt gaaggggccg acgaagtctt cggcggttac ttgtactttc
      841 acaaagcacc gaacaaagtc gagctgcacg aagagacggt tcgcaagctc agcatgctgc
      901 acaaatacga ctgtctgcgg gccaacaagg cgatggccgc ctggggcgtc gaagcgcgcg
      961 ttccgttcct cgatcgggag ttcctcgacg tggccatgac ggttgatccg gccgagaaaa
     1021 tgtgcggcgg gggcaagatc gaaaagaaga tgctgcgtga ggcgttcaaa ggctacattc
     1081 cggatgaaat tctctggcgc cagaaggaac agttctcgga tggcgtgggc tactcctgga
     1141 tcgatacgct gaa
//
LOCUS       DAORHD010001638         1153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_181472_length_1153_cov_2.512750, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001638 DAORHD010000000
VERSION     DAORHD010001638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181472_length_1153_cov_2.512750"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PKO06_24105"
     CDS             <1..247
                     /locus_tag="PKO06_24105"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNV72813.1"
                     /translation="PFPTQKLAALVEASKGRVLVLEEHSPFGGLGEATASALAGKIRE
                     FHHVAVDKVPRSGSPEEMLESFGLSAKTIATRIKSLL"
     gene            569..>1153
                     /gene="udk"
                     /locus_tag="PKO06_24110"
     CDS             569..>1153
                     /gene="udk"
                     /locus_tag="PKO06_24110"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNV72814.1"
                     /translation="MYIIGICGLSGSGKSSVSRVIEERYPGKVAVLQHDMYYHAKKNR
                     PNLTNYDQPDALETDLLISHIHELRSEKAIERPIYDFKVSDRQKETVRVEPHPILVVE
                     GLLIFCIPELMPLFDLRIYIDVPLDVAFIRRIKRDHIDRGRTVKSIVDQYEKTVRDSA
                     INLVQPSRYVADVIIPGGVSNRVGLEVLFGKLNDL"
BASE COUNT          254 a          287 c          303 g          309 t
ORIGIN      
        1 tccgtttccg actcagaaac tggcggcatt ggtggaggct tcaaaggggc gcgtgctggt
       61 actcgaagaa cactctccgt ttggtggtct tggggaagcc acagccagcg ctcttgccgg
      121 caagattcgc gagtttcacc atgtggcggt cgacaaggtt cctcgatctg ggtcgccgga
      181 ggaaatgctc gaatcgttcg gactttcggc gaagacgatc gctaccagaa tcaagtcact
      241 tctgtaacct gatcgctcgc aggggccggg acttcgtctc tgcccctgct ttttttttct
      301 ttttttggcg ctgtatacga ctgtaggatt ctgcacatgt ctgagtttgt ctgttttcgt
      361 tgtcgaatgt ctctgggcga cactgcagaa atcgttaaca aaaggtcatt tccggccgaa
      421 aaggttcatg gatttgtttg cctccttata acttcagacc ggggaatcat ccccggtctc
      481 tttttgtaca ctcgattccg ttcccggtag tgaagaattg ctctaatcat ggtatgatcc
      541 tgcccgggaa taatcccggg ggtgaatttt gtacattatt ggaatctgcg ggctctctgg
      601 ttctgggaaa agcagcgtgt cgagggtcat cgaagagcga tatcccggca aagtggcggt
      661 tcttcagcat gatatgtact atcatgccaa gaaaaaccgt ccgaacctca caaactacga
      721 tcagccagat gcactcgaga ccgatctgct gatctcacac atacacgaac tgcgcagcga
      781 gaaagcgatt gaacgcccga tctatgattt caaagtttct gaccggcaaa aagagactgt
      841 gcgcgtcgag ccgcatccca ttctcgtggt cgaagggttg ctcattttct gcattcctga
      901 actgatgcca ttgtttgacc tgcgcatcta tatcgatgtt cctctcgacg tggctttcat
      961 acgtcgtatc aagcgagatc acatcgatcg tggccgaacc gtgaagagca ttgttgatca
     1021 gtatgagaaa acggtgcgtg attcagccat caatctggtg cagccgtcgc gctacgtcgc
     1081 cgatgtgatt attccgggtg gtgtcagcaa ccgcgtcgga ctggaagtac tattcgggaa
     1141 actgaacgat ctt
//
LOCUS       DAORHD010001639         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_181931_length_1151_cov_2.994526, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001639 DAORHD010000000
VERSION     DAORHD010001639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181931_length_1151_cov_2.994526"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKO06_24115"
     CDS             <1..771
                     /locus_tag="PKO06_24115"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV72815.1"
                     /translation="RNRETAEWLKTNGVEVFFDPEDCTTHAKAVCADNQTLLVGSTNL
                     SNSSVGRNNETNVLIRSPEIGAGFAEYFHLLIHDQPANQSWIDPTGTATLLADASYLE
                     ALHEMIRTASDSLDVIMYSCSFSDRRRNEVWGILEALAARAKDGVKVRLLFDQSRSFS
                     RHITWGNLRAAAYLRQMGVETVSFDDPTRFTHAKAVVKDAREVLLGSTNWYFTDHGRN
                     RQLNVLIRNPETARWFQEYIANKTTTDGQAPHESFRSE"
     gene            877..>1151
                     /locus_tag="PKO06_24120"
     CDS             877..>1151
                     /locus_tag="PKO06_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72816.1"
                     /translation="MKKLSMLVVCALVLSCLFVPGAAQAQDAKIRLVDNTHVPQILLV
                     IHTYGTAQFVRDLFSVVKHINESNQLPPAEQLKLHIISSGGDPLSQLG"
BASE COUNT          253 a          313 c          325 g          260 t
ORIGIN      
        1 cggaatcgtg aaaccgcgga atggttgaag acaaatgggg tcgaagtgtt ttttgacccg
       61 gaagattgta cgactcatgc gaaggcggtg tgcgctgata atcagacatt gcttgtcgga
      121 tcgacgaatc tttccaatag ttccgttggt cgcaacaacg aaacgaacgt gttgatacgc
      181 tcgccggaaa tcggcgcggg cttcgccgaa tatttccatc tgctcattca tgatcaaccg
      241 gcgaaccagt cgtggatcga cccgacgggt acagcgaccc tgctggccga tgcgtcctat
      301 cttgaagccc tgcatgagat gatcagaacc gcatctgaca gtctcgatgt catcatgtat
      361 tcctgttcgt tttccgacag gcgtcggaac gaggtctggg gtatactcga agcgctcgcc
      421 gcgagagcga aagacggagt gaaagttcga ttgctgtttg accagagtcg ttcgttttcg
      481 cgtcacatca cctgggggaa cctgcgagct gccgcctatc tgcggcagat gggggtcgaa
      541 acggtgagct tcgatgatcc caccagattt acccatgcca aggcggtcgt gaaagatgca
      601 cgcgaggtgc ttctcggctc gacgaactgg tacttcaccg accatggccg caaccggcag
      661 ttgaatgtgc tgatcagaaa ccctgagacc gcccgctggt tccaggaata catcgccaac
      721 aaaaccacga ccgacgggca ggcgccccac gagtcttttc gctccgagtg agccatgaag
      781 cccggcccac gccaccatct cgaaccgatt tgccaagatg aaattggggg agtatactcg
      841 tttgtacaga ttgttaccca caaggagcgg aggagtatga agaagctgag tatgttggtt
      901 gtgtgtgccc ttgttctctc ttgtctgttc gtgccgggcg cggcacaggc ccaggatgcc
      961 aagattcgcc tggtcgacaa cacgcatgtg ccgcagattt tgctggtgat ccacacctac
     1021 ggcacggcgc agtttgttcg cgatctgttc agtgtcgtca agcacatcaa cgagtccaat
     1081 cagcttccgc ccgctgagca gttgaagctg catatcatct cttccggcgg tgatccgctt
     1141 tcgcaactcg g
//
LOCUS       DAORHD010001640         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_181942_length_1151_cov_2.892336, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001640 DAORHD010000000
VERSION     DAORHD010001640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_181942_length_1151_cov_2.892336"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PKO06_24125"
     CDS             complement(<1..408)
                     /locus_tag="PKO06_24125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72817.1"
                     /translation="MKRLLLLLTLGILFSTTLTAAEQTLVPGTSQRLHLSKDVPKAAI
                     TFWYPASRAVAVRVKANVPGTDVSLSGKREEKFGSLKVAAKFQDSFVTDVLADECFDS
                     IPPLPGTWTIELTLRGRKPDTSLEISLEEVGVLP"
     gene            556..>1151
                     /locus_tag="PKO06_24130"
     CDS             556..>1151
                     /locus_tag="PKO06_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72818.1"
                     /translation="MMIQDLVPGEYWPLVRAVPQMLAAVTSLAIGVFALVKAPKKPLN
                     RILAGLAAASFHYNLFQVLFNFFPSITLTRIGYVGAVFLIPLLAEFPNALISSEYASR
                     RTRTITLFCTGFFLAALPTEYLFLPDLDTSASLPLALGGPLMPVFAVVVGLVLVRVAF
                     KLVQALWRCTDDIMRRRLEFVLLGEAIYAVCALHDVLLR"
BASE COUNT          209 a          318 c          351 g          273 t
ORIGIN      
        1 cggcaacacc ccgacttctt cgagagagat ctccagtgac gtgtcgggtt tgcgtccccg
       61 cagtgtcagc tcgatcgtcc aggttcccgg aagcggtgga atgctgtcga agcactcatc
      121 cgcgagaaca tccgtgacaa acgaatcctg aaacttcgcg gccaccttca gagatccgaa
      181 cttttcctcg cgttttcccg acagggagac atcggttccg ggcacgtttg ctttcacgcg
      241 cacggcgacc gcacgtgagg ccggatacca gaacgtgatg gcagccttgg gaacgtcctt
      301 gctcaggtgc agacgttgag atgttcctgg gacaagcgtc tgttcggcgg cggtgagagt
      361 cgtggagaag aggattccga gagtgaggag caggagaagg cgtttcattg gatggtccat
      421 cgtcgtgaga attgtcggca accgggaaaa acaataccgt ttcgacttgt tcgtgaggtt
      481 cgttatagta agaaaagccg ctcgaaaagg caagcgggtt gacctgacga gggaatcagt
      541 gtagtcctgt aacacatgat gattcaggat ctggttccgg gcgaatactg gccgctggtg
      601 cgggcggtgc cgcagatgct cgcggccgtg acctcactgg cgatcggtgt cttcgcgctg
      661 gtgaaagcac cgaagaaacc tctcaaccgt attctcgccg ggctggccgc tgcttccttt
      721 cactacaatc tgtttcaggt gctcttcaat ttcttcccgt cgatcactct gacacgcatc
      781 ggctacgttg gtgccgtctt tctgattccc ttgctggcag agttcccaaa tgcgttgatc
      841 agcagcgagt atgcgtcgag acgaactcgg acgatcactc tattctgtac cgggttcttt
      901 ctggccgccc tgccgaccga gtatctgttt ctccctgacc tcgacacctc cgcgtcgttg
      961 ccactggcgc ttggcgggcc gctgatgccg gtgttcgccg tggtcgtggg gcttgtgctc
     1021 gtgcgcgtcg cgttcaagct ggtgcaggcc ctgtggcgat gcacggacga catcatgcga
     1081 cgacgcctgg agttcgtgct gctcggtgag gcgatctatg ccgtgtgtgc cctgcatgat
     1141 gtgttgctgc g
//
LOCUS       DAORHD010001641         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_182743_length_1148_cov_3.319305, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001641 DAORHD010000000
VERSION     DAORHD010001641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182743_length_1148_cov_3.319305"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKO06_24135"
     CDS             <1..261
                     /locus_tag="PKO06_24135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HNV72819.1"
                     /translation="ASADNEQNSIGIDDNGYKVLVKQIAGIVARRIVCWADTGQSLAR
                     GERFGLIRFGSRTELFLPLDARIEVTPGQKVEGGATIIGRRH"
     gene            245..1117
                     /gene="pssA"
                     /locus_tag="PKO06_24140"
     CDS             245..1117
                     /gene="pssA"
                     /locus_tag="PKO06_24140"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNV72820.1"
                     /translation="MADVTDTQNGQTSAPTPGSTPVTTPGIAPVATSLPAAENEPRLK
                     GKWRHLSILPNVCTSMNLFCGYLSILFTSRQEFLEAGWMIVLAVIFDILDGRIARLTS
                     VSSRFGAELDSLADLVSFGVAPAFLVYSRYLSDYRIIGLISTSMFVLCGALRLARFNI
                     TPPSEKDVFIGLPIPAGAGILCTLTIFEMQFVQVFRVPDVIIPIVVIITSLLMVSQIE
                     YPAMKKSKKTGAQRQLLVVFFVAALVIAPPVSLFCYSWGFAIYGLVMFFFRKLIGLFR
                     RRPQEPGVMPSGTI"
BASE COUNT          201 a          336 c          314 g          297 t
ORIGIN      
        1 gcctcggcag ataatgaaca gaactcgatc ggcatcgatg acaacgggta caaagtcttg
       61 gtgaagcaga tcgccggcat tgtggctcgc cgcattgtct gctgggcgga taccgggcag
      121 agtctggctc ggggtgagcg ttttggcttg attcgctttg gctcgcgcac tgaactgttt
      181 ctgccgctcg atgcgcgcat cgaagtgacc cccggacaaa aagtcgaggg aggagcaacc
      241 atcattggca gacgtcactg acacacagaa cggacaaacc tcggctccca cacctggaag
      301 tactcccgtc accacgcctg gaatcgcgcc ggtggcgact tctctgccag cggcggagaa
      361 tgagccccgc ttgaagggaa agtggcgcca tctgtcgatt ctgccgaatg tctgtacctc
      421 gatgaacctg ttctgcgggt atctgtcgat tctgttcacc tcgcgccagg agttcctcga
      481 agccggatgg atgatcgttc tggccgttat tttcgatatt ctcgatggcc gcattgcgcg
      541 tctgacgtcc gtcagttccc gcttcggtgc cgagcttgat tcgctggccg atctggtcag
      601 tttcggagtc gctccggctt ttctcgtcta ttcacgctat ctctctgact atcgcatcat
      661 cgggctcatc tcgacctcga tgttcgttct gtgcggcgct ctgcgtctgg cccgcttcaa
      721 catcaccccg ccatccgaaa aagacgtctt catcggtctg cccattccag ccggtgcagg
      781 aattctgtgc acgctgacga tctttgaaat gcagttcgtt caggtgttcc gcgttcctga
      841 tgtcattatt ccgatcgtcg tgatcattac ctcactcttg atggtcagcc agatcgaata
      901 cccggccatg aaaaagtcga agaaaaccgg ggcacaacga cagcttctgg tggtcttctt
      961 cgtggcggcc ctggtgattg ctcctccggt gagcctgttc tgctattcgt ggggctttgc
     1021 catctacggg ctggtcatgt tcttctttcg gaaactcatc ggtctgtttc gccgtcgacc
     1081 gcaagagcct ggggtcatgc cgtccggcac gatctgaccc tttcggcggc ttctcttcat
     1141 ctctgttg
//
LOCUS       DAORHD010001642         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_182755_length_1148_cov_3.212260, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001642 DAORHD010000000
VERSION     DAORHD010001642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_182755_length_1148_cov_3.212260"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKO06_24145"
     CDS             complement(<1..546)
                     /locus_tag="PKO06_24145"
                     /inference="COORDINATES: protein motif:HMM:NF024412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV72821.1"
                     /translation="MSPKAIDRLFLVRQVIEDRYTVNEAARAMKLSERQVYRLLARFS
                     KEGPTCLIHGNRGRSPSHSTSAEVKEHIVALAQGVFRGANFTHFSELLEEQFQIVRSP
                     KTIGRILKKKGIVSTKSHSKPRRFRKRKRRPREGELVQMDASPFAWLEDRGPMMSLHG
                     AIDDATGKVLGLYFRPTEDTLG"
     gene            667..>1148
                     /locus_tag="PKO06_24150"
     CDS             667..>1148
                     /locus_tag="PKO06_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72822.1"
                     /translation="MKRLLFIRALVALLILSLSLAQPLLAREEGGLFSKLKAKASEKI
                     SQLKSKDWWKITLSGKATAFVGGKVIGLAGMALGGALGLLAGPAGAAVGALVGYRLGS
                     IAGSTFARPIGKDLARDKIAGDDDLTWSDVKDSWNSQDKKALAVEAGAGIVGDFLGEV
                     "
BASE COUNT          249 a          299 c          331 g          269 t
ORIGIN      
        1 accaagagtg tcttcggttg ggcgaaaata caagccgaga accttcccgg tcgcatcatc
       61 gatggctcca tggagagaca tcatcggacc tcgatcttca agccaggcaa atggactggc
      121 gtccatttgt acaagttctc cctcgcgggg gcggcgcttt ctcttccgaa accttcgagg
      181 ctttgaatga gatttcgtcg acacaattcc cttcttcttg agaatgcggc cgatggtttt
      241 cgggctcctg acaatctgga actgctcttc aagcagttct gaaaagtgag tgaagtttgc
      301 accccggaag actccttggg cgagagccac aatgtgttct ttcacttctg ccgaggttga
      361 gtgagaagga gatcgaccac ggtttccgtg aatcagacac gttgggcctt ctttactgaa
      421 ccgcgcaagt agacggtaga cctgtcgctc ggataacttc atagcccttg cggcttcatt
      481 cacggtgtaa cgatcttcga taacctgcct cacgaggaac aacctgtcga tagctttggg
      541 actcaagctc atcgtttcca taccctgaca ttttcaatgt cccgttaaca ggcgtgattc
      601 gggggcttcc ggggaggggg ggattctgtg tacaatagga aaaatctgtc gttcggagag
      661 accgttatga agcgcctgct gttcattcgc gctcttgtcg ctctgctgat cttgagtctc
      721 agtctggctc aacctctgct ggcgagagaa gaaggcgggc ttttctcgaa actgaaagcc
      781 aaggcttcag aaaagatttc acagctgaaa agcaaggatt ggtggaagat tactctgagc
      841 gggaaggcca cggcgtttgt cggtggaaaa gtgatcggcc ttgccggaat ggctctcggt
      901 ggcgcactcg gactcctggc cggaccagcc ggggccgcgg tcggcgcact cgtcggatac
      961 cggctcggca gcatcgctgg cagcaccttt gcgcgcccca tcggcaaaga cctggcccgc
     1021 gacaagattg ccggagacga cgatcttacc tggtcggacg tcaaggattc ctggaacagt
     1081 caggataaaa aggcactggc tgtcgaagcg ggtgcaggca tcgtcggcga tttcctgggg
     1141 gaagtctt
//
LOCUS       DAORHD010001643         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_183660_length_1145_cov_2.850459, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001643 DAORHD010000000
VERSION     DAORHD010001643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_183660_length_1145_cov_2.850459"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKO06_24155"
     CDS             <1..249
                     /locus_tag="PKO06_24155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72823.1"
                     /translation="DACNSFSAQEGQRLVQTMHRDFSRDAEIVEVNHKNYTDPANELF
                     SQVNNMVCVYNARLRKASPALFGGSDVAATIRRLLDER"
     gene            280..921
                     /locus_tag="PKO06_24160"
     CDS             280..921
                     /locus_tag="PKO06_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72824.1"
                     /translation="MDSSGAVYAKFPAGGVEALEQAVSEARKASRHTSPIKVLGGDPI
                     RHAYAVVDRWGRSDNMPIWAEGPDLTTAFELVELSRLVGDVVAFYTIDDGLSMGIYGA
                     WKDGRLVRNLQWMDDQWQVVEGDPQPWEKPLFSSESREACLECARDNGWDESEVQKMF
                     QAGVLQPGFRFPIAHGLVRHINAACPGPLYGFEPWPARRTLLEDLGLVKKRPR"
BASE COUNT          248 a          321 c          360 g          216 t
ORIGIN      
        1 gatgcctgca acagtttcag cgctcaggaa ggacagcgac tggtgcagac gatgcaccga
       61 gacttctcgc gcgatgccga gatcgtcgaa gtgaaccaca agaactacac cgatccggcc
      121 aatgagctgt tttcgcaggt caacaacatg gtctgcgtct acaacgctcg attgcggaaa
      181 gcctctccgg cgctgttcgg tggttcagat gtcgcggcga ccattcgccg actgctcgac
      241 gaacgttgat gatcgaaaac cctggaagga gaacgaaaga tggatagcag cggagccgtg
      301 tatgccaagt ttccggccgg gggagtggaa gccctcgaac aggcggtgag cgaggcgcga
      361 aaagcgagcc gacataccag cccgatcaag gtgctggggg gtgaccccat ccgtcatgcc
      421 tacgctgtgg tcgatcgctg gggtcgctcg gacaacatgc cgatctgggc cgaagggcct
      481 gatctgacga cggcgttcga gttggtcgaa ctgtcgcgcc tggtcggcga cgtcgttgcg
      541 ttctatacga ttgatgacgg cttgtccatg ggaatctacg gggcctggaa ggacgggcga
      601 ctcgtacgca acctgcaatg gatggacgac cagtggcagg ttgtcgaagg cgaccctcaa
      661 ccgtgggaaa aacccctgtt cagttcagag tcacgggaag cctgtctcga gtgcgcccgc
      721 gacaacgggt gggacgaaag cgaagttcag aagatgtttc aggccggtgt ccttcagccc
      781 ggatttcggt tcccgattgc acacggtctc gtgcgccata tcaacgccgc ctgtcccggc
      841 ccgctgtacg gctttgaacc gtggccggcc cgccgaaccc tgcttgaaga cctcggcctg
      901 gtcaaaaaac gcccgagata agcccgcgag agaatcactc accatgcgcg aagctcaatc
      961 cgacaggatg aagatccagc gggggttgag gttgatctcg agcgcggtgt tctcggcgat
     1021 accaatggtg cggcggatta tctgcgaggg taggggtgaa gcgtcagccc ctgaatgaaa
     1081 gaaaagtctc tcttgtttca ttcaggggct gacgcttcac ccctaccctc gcagataatc
     1141 cgccg
//
LOCUS       DAORHD010001644         1144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_184015_length_1144_cov_2.386593, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001644 DAORHD010000000
VERSION     DAORHD010001644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184015_length_1144_cov_2.386593"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..>1144
                     /locus_tag="PKO06_24165"
     CDS             210..>1144
                     /locus_tag="PKO06_24165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV72825.1"
                     /translation="MSINKRLLALFSIIILFACASLVQAANKNPLLRVTFLDVGQGDS
                     VLIRTAEKTILLDAGDDRSNAANVSIIPYLKKEGIKKIDTCIISHPHRDHFGGFIDLV
                     QAIPIGEFLYAADFMTNANDGESSGNDAQLYQKLHFAIMEKGIAYNQVTEETKFDWGT
                     GIKVDLFHVAESVGIRDEVASAPKVDGWKLNANNQSLIFRVSAGKISYMFSGDAEKEV
                     EANMVAKFGSKLRATVMKSGHHGSKTSNSPSFLENVFGGPNEVTGPRYAVISVGAKNS
                     FKHPNQETLDNYNYWKVKYFRTDLDGPVDSFTDGQT"
BASE COUNT          274 a          336 c          287 g          247 t
ORIGIN      
        1 gtttcaacga attcggtcgc aaagaagcgc gccaagcctg acttaccatg gcctggcgcg
       61 cttttatttg acaccaagac agtatcctgt acttcgcaat tttttgcttc tgaagcgaat
      121 ttttctgggt acatccattg acaccctcag gaatcattcg tagaattgac atatatcgta
      181 ccctgtgccg ctataaggag tatctggtaa tgagtatcaa taagcggttg ctggcactct
      241 tcagcatcat cattttgttc gcttgcgcgt cgctggttca ggcggccaac aagaaccccc
      301 tgctgcgcgt cacctttctt gacgtcggtc agggtgactc ggttctcatt cgcaccgccg
      361 agaaaaccat tcttctcgat gctggcgacg accgctccaa cgcggcgaat gtctcgatca
      421 tcccgtatct gaaaaaagag ggcatcaaga agatcgacac ctgcatcatc tcgcacccgc
      481 accgcgacca cttcggcggg tttatcgacc tcgtgcaggc cattcccatc ggcgagtttc
      541 tgtatgccgc ggacttcatg accaacgcga acgacggcga atcaagcggc aacgacgccc
      601 agctgtatca gaagctgcat ttcgcgatca tggaaaaggg cattgcgtac aatcaggtca
      661 ccgaagagac caagttcgac tggggcaccg gcatcaaggt cgatctgttt catgttgccg
      721 aatcagtcgg cattcgcgat gaggtcgctt cagcgccgaa ggttgacggc tggaagctga
      781 acgccaacaa ccagtcgctc atctttcgcg tctcagccgg aaagatcagc tacatgttca
      841 gcggcgatgc cgaaaaagaa gttgaagcca acatggtggc caagttcggc agcaaactgc
      901 gcgccacagt catgaagtcc ggccatcacg gcagcaagac ctcgaactct ccgtcgttcc
      961 tcgaaaacgt gttcggcggc ccgaacgagg tcaccggccc gcgctacgcc gttatctcgg
     1021 taggcgccaa gaactcgttc aaacacccca accaggaaac cctcgacaac tacaattact
     1081 ggaaggtgaa gtatttccgc accgatctcg atggcccggt cgacagcttc accgatggcc
     1141 agac
//
LOCUS       DAORHD010001645         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_184217_length_1143_cov_3.474265, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001645 DAORHD010000000
VERSION     DAORHD010001645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184217_length_1143_cov_3.474265"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..>1143
                     /locus_tag="PKO06_24170"
     CDS             149..>1143
                     /locus_tag="PKO06_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72826.1"
                     /translation="MISSIQWTKRWLELLKKAGDWRHKMTIARAEAMLSAGQALLAGV
                     NVPPAPVEVVEVPEPTTARPSTPSPTGQEQTAADDSPALADPQVNPVPPAQNAKLLEI
                     NYGLTRIQQRFQQRESIFKVLESIHWTDSRIRDFESNVQDEDGRKQAIEARETMRSLR
                     DRAAQVLISDITYGLQKFEERQRANESPATLRNTISWTELRLRELKRLNDPASRAFTA
                     DAEQRLQQARTRVPAAAPSVPYLGQLNNTLNPYGTCQNTSIAMTLRAYGWQGTPDDLT
                     REFGNGLGKEPNGVAEIINTIAARNGLKIKAVPHTQATPTEFRESISRGPVVTHG"
BASE COUNT          285 a          320 c          322 g          216 t
ORIGIN      
        1 gattgtctcg ttagcatcag caaggtagtt ggagacatag acctcaaagc cgtagatgct
       61 gtagataaat cgcatctgat taccaaagcc ggcattgata acgttatccc aaatcggacg
      121 agcgctgaat gcagcgtcac cggccgagtt gatttcaagc atccagtgga ccaaacgctg
      181 gctcgaactg ctgaaaaagg cgggtgactg gcgccacaaa atgaccattg cccgtgctga
      241 agccatgctg tcggctgggc aggcgcttct ggccggggtc aacgtgcccc cggcaccagt
      301 tgaagtggtc gaggttcctg aaccgacgac ggcccgtccc tcgacgccat cgccaaccgg
      361 tcaagaacaa acggctgctg acgattcccc ggcacttgct gatccgcagg tgaaccccgt
      421 gccgccagcg cagaatgcga aactgcttga gattaattat ggtctgaccc gtattcaaca
      481 gcgttttcaa cagcgggaaa gcatcttcaa ggttctcgaa tcgattcatt ggacagactc
      541 gcgcattcgg gacttcgaat cgaacgtaca agatgaagac ggtaggaaac aagcaataga
      601 ggctcgagag accatgcggt cattgagaga tcgtgccgcg caggtgttga tcagtgacat
      661 tacctatggt ctgcagaagt tcgaagaacg tcagcgcgcg aatgagtccc cagcaactct
      721 gcggaacacc atctcctgga cagagctgcg cctgcgggag ctgaaacgcc tgaacgaccc
      781 ggcatcgcgt gcatttaccg ccgatgctga acagagactg cagcaggctc gcacccgggt
      841 tccagctgct gccccttcgg tgccgtatct gggacagctc aacaatactc tgaatccgta
      901 tggaacctgc cagaacacct cgattgccat gacattacga gcctatggat ggcagggcac
      961 gcctgacgat ctcacgcgag agtttggtaa cggactcggc aaggaaccga acggcgtggc
     1021 agagatcatc aacacgatcg ccgcgcgcaa tgggttgaag ataaaagcgg tgccgcacac
     1081 tcaggcgacg ccaaccgagt ttcgtgaatc gatcagtcgc ggtccagtcg tgacccacgg
     1141 atg
//
LOCUS       DAORHD010001646         1143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_184417_length_1143_cov_1.756434, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001646 DAORHD010000000
VERSION     DAORHD010001646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184417_length_1143_cov_1.756434"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..234)
                     /locus_tag="PKO06_24175"
     CDS             complement(7..234)
                     /locus_tag="PKO06_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72827.1"
                     /translation="MSNPVINIKYMLHDSDNPCDIGRCLDDLHAYLSVQPCMADFARL
                     NDFFGIDNSYEFEQLLSDLFQYCDAHGIEVD"
     gene            complement(411..701)
                     /locus_tag="PKO06_24180"
     CDS             complement(411..701)
                     /locus_tag="PKO06_24180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72828.1"
                     /translation="MNTQARHAAPPRRAEFRIIDHAPPSLEEAEAFVGGKVELLALKD
                     DDEDIQILMNAQWMEKNLPINHPATDVSGTMVFGNAILLVEDALWKNWQGYS"
     gene            complement(779..>1143)
                     /locus_tag="PKO06_24185"
     CDS             complement(779..>1143)
                     /locus_tag="PKO06_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72829.1"
                     /translation="AEDAVKRIKICYRPQAQLKGHLEVTEAIFDALVECARARDELCI
                     LVTERCHTNKQSLRLMRERVVQLMKQDNVCAYTALITWCEELYEPRKREQFIQDWMLE
                     RQVSREAAEAAYEFWGGF"
BASE COUNT          261 a          304 c          291 g          287 t
ORIGIN      
        1 gagtggtcag tcgacttcga tgccgtgagc gtcgcagtac tggaagagat ctgagagcag
       61 ttgttcgaat tcgtacgagt tgtcgatgcc gaagaagtcg ttgagacgag caaagtctgc
      121 catgcagggc tgaacactga gataagcgtg cagatcgtcg agacatcgac cgatatcgca
      181 ggggttgtcg ctgtcatgaa gcatgtactt gatgttgatc acggggttgg acataccttc
      241 ctcctcgcct cgcttatgat tttgggtcgc cgctatccat gacattccgg aaaacaacgg
      301 ttgtatgttt caatgatcac gaacagcggt caggcggcca tccaatcaca cgaggaaatg
      361 gatcggtgcg atacatggcc tccatggcct cgcggtctgg acatggttgc tcaagaatat
      421 ccctgccagt ttttccagag cgcatcttct accagcagaa tggcatttcc gaagaccatc
      481 gttccagaaa cgtctgttgc tggatggttg atgggcaagt tcttttccat ccattgggca
      541 ttcatgagaa tctggatgtc ttcatcgtcg tccttgaggg caagaagctc taccttgccg
      601 ccgacaaacg cttccgcctc ttcaagcgag ggaggagcgt gatcaatgat cctgaactct
      661 gcccgtcgag gtggggctgc atgtctggcc tgagtattca tatgtcctcc atgaacaacc
      721 tgatttgatc tctcgatgaa catactgcaa acgagtgtga caggaaaatc ggctcgagtc
      781 agaagccacc ccagaactcg tatgccgctt ccgctgcctc acgcgaaacc tgcctttcga
      841 gcatccaatc ctgaatgaac tgttcacgct tgcgaggttc atacagttcc tcacaccacg
      901 tgatcaatgc agtgtaggcg cagacgttgt cttgcttcat cagctgaacc acacgctctc
      961 gcatgagcct cagagattgt ttgttcgtat gacatcgctc tgtgaccaga atgcacaact
     1021 cgtctctggc cctcgcgcat tcgaccagag catcgaagat ggcttccgtg acttcaagat
     1081 gtcccttcaa ctgtgcctga ggtcgataac agatcttgat gcgcttcact gcatcttccg
     1141 cac
//
LOCUS       DAORHD010001647         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_184632_length_1142_cov_2.197792, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001647 DAORHD010000000
VERSION     DAORHD010001647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_184632_length_1142_cov_2.197792"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PKO06_24190"
     CDS             complement(<1..1124)
                     /locus_tag="PKO06_24190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV72830.1"
                     /translation="MGFAVWLQQRLVSNQDALEALLSRQPPAPDPETSPAAAEAFLRT
                     LFDQGSFPAPALGHSGLNKLAQRWEARFYADLLGHSGKRQPWRALADRLSLEPAPARS
                     WASWPTQPLPWIGEPETPALRLPFRKVHGHVSSDGLPVLSMSHLLGMAEGRTHPVTAL
                     RAPSVDLYVQTRHHRVQVVYHPEARGSCVIEGQITDAFGTPASNAHVLALRLEGEYWA
                     TGTVDGNGRYRLTDLPPGTYSLEITVGYQFGRIQAVHETGVYVATETVVDRVFLPPAS
                     ASLHLSVAPSTTPRELVLSGDALWVAWTGPEGTLPGDHPPPGFDLTVLDSDETFVWQS
                     GKLRDASGTARPAMKAALTETQPVWKVAWNAHHPIDAGQK"
BASE COUNT          222 a          332 c          357 g          231 t
ORIGIN      
        1 tgtttttgcc cggcatcgat gggatgatgc gcattccagg cgaccttcca gacaggttgt
       61 gtctcggtta gagccgcttt cattgccggt cgcgccgttc ccgaggcatc acgtaacttc
      121 ccggactgcc acacgaacgt ctcgtcggaa tcgagaacgg tgagatcgaa accgggcggt
      181 ggatggtctc ccggcagtgt gccttcgggc cccgtccagg ctacccagag cgcatctccg
      241 cttaaaacaa gttctctcgg cgtcgtggag ggggcgacgc tcagatggag ggatgccgaa
      301 gccggcggca gaaacaccct gtcgacgact gtttccgtgg ctacatacac acccgtctcg
      361 tggacggcct gaatacgccc gaactgatag ccgacggtga tttccagcga ataggttccc
      421 ggcggcagat ctgtcagccg ataccggcca ttcccatcca ctgtcccggt tgcccagtac
      481 tcaccctcga ggcgaagggc aagaacatgc gcgttgctcg ccggtgtgcc gaatgcatcc
      541 gtgatttgtc cctcgatgac acaggatcct cgcgcttccg ggtgataaac aacctggacg
      601 cgatggtgcc tggtctgaac gtacagatcg acactgggcg ccctgagtgc cgtcaccggg
      661 tgcgttcggc cttcggccat gcctagcagg tggctcatcg acagtaccgg cagtccgtca
      721 cttgagacgt gaccgtgcac cttgcggaat ggcagacgca gtgccggagt ttcaggttct
      781 ccgatccagg gaagcggttg agtcggccag gaagcccatg atctggcggg agctggctcg
      841 agagacagac gatcggcaag agctcgccag ggctggcgtt taccggaatg tccgaggaga
      901 tccgcgtaaa agcgcgcctc ccatcgttgt gccagtttgt tcaaccctga gtgtccaagt
      961 gcaggcgccg gaaacgatcc ttgatcgaag agggttcgca gaaatgcttc ggcggcggct
     1021 ggggatgtct cggggtccgg ggccggtggc tgtcgactca ggagtgcctc aagagcatcc
     1081 tgattgctga caagtctttg ttgaagccag actgcgaatc ccaaagatgg tacaaattcc
     1141 gt
//
LOCUS       DAORHD010001648         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_185610_length_1138_cov_4.292705, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001648 DAORHD010000000
VERSION     DAORHD010001648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185610_length_1138_cov_4.292705"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1138)
                     /gene="murJ"
                     /locus_tag="PKO06_24195"
     CDS             complement(<1..>1138)
                     /gene="murJ"
                     /locus_tag="PKO06_24195"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNV72831.1"
                     /translation="PEKYDTTVTMTRIMFPFLLEVSWAAVVMGMLNSFGSFFVPAIAP
                     VFLNLAMIAAGFVLCPLAVWYGWPAIVGMAIGAMLGGFLQFLVQIPALWKMGFRPGWS
                     WNLSDPGLQRIFALMIPGTIGMAATQINVVVSTWLATSQGTGAVSWLNYAFRLMQLPL
                     GLFGVAIAQATLPVVSRQAADGKLAEMKQTLADSLRLTVFINLSASLALVACAEPIIR
                     LLFEHGRFTPDDTLATAAALRAYAVGLLFFSVIKVMGPSFYALGDTRAPVRASLSSVV
                     ANVILNLALIGPLGYWGLALGGSLASGLNAGMLFWSIRRHLGGWSETGLFGAMGKTLI
                     CSTISSFVMYYSLGLLPSLFAVSDQIKLVRLLIEAGSLGIAGILD"
BASE COUNT          284 a          364 c          329 g          161 t
ORIGIN      
        1 gatcaagaat acccgcgata ccaagacttc cggcctcgat cagcaggcgt accagtttta
       61 tctggtctga aaccgcgaac aaagacggca gcaggccaag ggaatagtac atcacgaacg
      121 aggagatggt gctgcagatc agtgttttgc ccatcgcgcc gaacagtccg gtctcggacc
      181 atccgcccag atgccgacga atgctccaga acagcatgcc ggcgttcaac ccggaagcca
      241 gcgatccgcc cagtgccagg ccccagtatc ccagcggccc gatcaaggcc aggttgagaa
      301 tcacgttggc caccaccgac gacagactcg cgcgcacggg ggcccgggta tcgccaagcg
      361 catagaacga cgggcccatc accttgatca ccgaaaagaa cagcaggccg accgcatacg
      421 cgcgcaatgc cgccgcggtc gccagcgtat cgtcaggcgt gaaccgtcca tgttcgaaga
      481 gcaggcggat gatcggctcg gcacacgcaa cgagagccag cgaggccgaa agattgatga
      541 acacggtcaa tcgcaatgag tcggccagcg tctgcttcat ctcggcgagt ttgccatcgg
      601 cagcctggcg cgaaaccacc ggtagcgttg cctgtgcgat cgccacgccg aacagcccga
      661 gcggtagctg catcaatcga aaggcatagt tcagccacga gaccgcgccg gtgccctgac
      721 tcgtcgccag ccaggtgctg accacgacgt tgatctgcgt cgccgccatg ccgatcgtcc
      781 cagggatcat caacgcgaaa atgcgctgca agccaggatc agagagattc caggaccagc
      841 ccggccggaa acccatcttc cagagagcgg gaatctgcac caaaaactgc agaaagccgc
      901 cgagcatcgc cccgatcgcc atgccgacga tggccggcca gccataccag acggccagtg
      961 gacacagaac aaaaccagcc gcaatcatgg ccaggttgag aaacaccggg gcaatcgccg
     1021 gaacgaagaa gctgccgaac gagttgagca ttcccatgac cacggccgcc cacgagactt
     1081 cgagcagaaa cgggaacatg atgcgggtca tggtcaccgt ggtgtcatac ttttcggg
//
LOCUS       DAORHD010001649         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_185714_length_1138_cov_2.464451, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001649 DAORHD010000000
VERSION     DAORHD010001649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_185714_length_1138_cov_2.464451"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1138
                     /locus_tag="PKO06_24200"
     CDS             <1..>1138
                     /locus_tag="PKO06_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNV72832.1"
                     /translation="VQALPYLMEFPHECSEQTFNRIYANSLARRIAMADPKIKKVFET
                     WKAMEAQGGTALKSNLEKNESLKNALLIETPWVLDAQDETARKHRVGLLFDSNRVDSE
                     LQRGLNRLRQMQLSDGAFPWFPGGRPSSFITLYITTGFGRLRHLGVDIEMDLALKAVG
                     HLDSWIRDVYNHLKRLDLLSQVNIGYETALYLYGRSFFLKERPIPSASKEAVDYFLGQ
                     AEKYWLKVGSRMSQAHVALGLQRFGRKEVPAKIVASLKERSVNDEELGLFWRDTEESW
                     WWYRAPIETQALMIEVFDEIANDAQAVEDAKVWLIKQKQTQDWKTSKATADAIYGLLL
                     KGVDLLASDKLVRAKLGGTDIQPPANVEAGTGYYEVKYAPAEIKP"
BASE COUNT          273 a          326 c          328 g          211 t
ORIGIN      
        1 gtacaagctc tgccgtatct gatggagttc ccgcacgaat gttcggaaca aacgttcaac
       61 cgcatttacg cgaactccct ggcacgacga atcgccatgg cggacccgaa aatcaagaag
      121 gtgtttgaga cctggaaggc catggaagct caaggcggta ccgctctgaa atcgaatctc
      181 gaaaagaacg agtcactcaa gaatgcgttg ctgatcgaaa cgccctgggt actcgacgcg
      241 caggatgaaa ccgcacggaa gcatcgcgtc ggcctgctgt tcgacagcaa tcgcgtcgac
      301 agcgagttgc agcggggcct gaaccgcctt cgccagatgc agctgagcga cggtgcattc
      361 ccgtggttcc ccggtggacg tccgagttcg ttcattacgc tctacatcac gacgggattc
      421 ggccgtctgc gccatctggg ggtcgacatc gaaatggatc tggctctcaa ggccgtcgga
      481 cacctcgatt cctggattcg cgacgtgtac aatcacctga agcgcctcga ccttctgagc
      541 caggtcaaca tcggctatga aaccgccctc tatctctacg gacgcagctt cttcctgaag
      601 gagcggccga ttccttctgc gagcaaggaa gcggtcgact acttcctcgg acaagcggaa
      661 aagtactggc tcaaagtcgg ctcgcgcatg tcacaggccc acgtcgctct gggtctgcaa
      721 cggttcggtc gcaaggaagt gcccgcgaaa atcgtggcga gcctgaaaga acgcagtgtc
      781 aacgacgaag aactcggact cttctggcgc gacaccgagg aatcatggtg gtggtatcgc
      841 gctccgatcg agacccaggc cctgatgatc gaagtgttcg atgagatcgc caacgatgcg
      901 caggcggtcg aagacgccaa ggtctggttg atcaaacaga agcagaccca ggactggaaa
      961 acctcaaagg ccacggcaga tgcgatttac ggcctgttgc tgaaaggcgt cgatctgctc
     1021 gcctctgaca aactggtacg cgccaaactt ggcggcaccg acattcaacc gccggcgaat
     1081 gtcgaggcag gtacaggcta ctatgaggtg aaatacgctc cagcagagat caaaccgg
//
LOCUS       DAORHD010001650         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_186235_length_1136_cov_3.135060, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001650 DAORHD010000000
VERSION     DAORHD010001650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186235_length_1136_cov_3.135060"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="PKO06_24205"
     CDS             complement(<1..940)
                     /locus_tag="PKO06_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72833.1"
                     /translation="MIFANLHDTSRHTVRATPVHHPYICLLLLVLSLTCGTALFAERA
                     VTERIASAPAPTPTRVASESSSDDRVPPEIRKLREMLKESDPAFSTADVERVFPQIVP
                     LVEKAAGRTFKKTPVWKIANRQQVAEVLAADILPQLDNLLPNATPEQRREVGLKQAQS
                     MAALMLGKYGFSDRTFYLLPRNFLPLFRVTKVPPSQVSSILQLIIAHELTHALQDQEI
                     GLLSKIANIRDNETGLAFNATIEGHAVVTMEKVGHELGLASSVIEASKLFAAGSVTFD
                     DPALQMINKVMSSQYESIYLGGKRFIEYWSQKSGPAM"
     gene            920..>1136
                     /locus_tag="PKO06_24210"
     CDS             920..>1136
                     /locus_tag="PKO06_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72834.1"
                     /translation="MQIRKNHDSPPENFGWVHDTANEEKGQKKCLTLWVGLLRGADFS
                     TTILEVPVRRLLMNELNQNAKATNRYNL"
BASE COUNT          244 a          300 c          355 g          237 t
ORIGIN      
        1 ccatggcggg gcctgacttc tgcgaccagt actcgatgaa tcgtttgcca ccgagataga
       61 tggattcata ctgcgaagac atcactttgt tgatcatctg cagcgctggg tcatcgaagg
      121 tgaccgaacc ggcggcaaac agcttcgaag cttcgatgac cgaactcgcc aggcccagct
      181 cgtgaccgac cttttccatg gtcacgacgg catggccttc gatagtggcg ttgaaagcca
      241 aaccggtctc gttgtcacga atgttcgcga tcttcgagag cagtccgatc tcctgatcct
      301 gcagggcgtg cgtgagttca tgggcgatga tcagttgcag aatgctggaa acctgactcg
      361 ggggcacctt cgttacgcga aacaagggca gaaaatttcg cggcagcagg taaaaggtcc
      421 tgtctgaaaa gccgtatttc cccaacatca gagccgccat cgactgggcc tgtttcagac
      481 caacctcgcg ccgctgttcc ggcgtcgcgt tgggcaacag attgtcaagt tgcggcagga
      541 tgtcggccgc gagcacttcg gccacctgct gacgattggc gatcttccag acgggagttt
      601 tcttgaacgt gcgaccagca gccttttcca ccaggggcac gatctgcgga aacacgcgtt
      661 cgacgtcggc ggtgctgaac gcggggtcgc tttccttgag catctcacga agttttcgga
      721 tttcgggtgg gactcggtcg tcagaactcg attcgctggc gacgcgggtg ggcgttggcg
      781 ctggagcgga ggcgattcgc tccgtgacgg cgcgctcggc gaagagggcg gttccgcacg
      841 tcaggctgag caccagaaga agcaggcaga tgtatgggtg gtgaaccggt gtggcgcgaa
      901 cggtgtggcg gctcgtgtcg tgcagattcg caaaaatcat gacagtcctc ctgaaaactt
      961 cggctgggtt cacgataccg caaacgaaga gaaggggcaa aagaaatgcc tgacgctctg
     1021 ggtggggctc ttgcgagggg cggatttttc cactacaata ctggaggttc ctgtcagaag
     1081 gctgctgatg aatgaactga accagaatgc gaaggcaacc aaccggtaca atctcg
//
LOCUS       DAORHD010001651         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_186287_length_1136_cov_2.639223, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001651 DAORHD010000000
VERSION     DAORHD010001651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186287_length_1136_cov_2.639223"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1136
                     /locus_tag="PKO06_24215"
     CDS             <1..>1136
                     /locus_tag="PKO06_24215"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72835.1"
                     /translation="MTSLGDTQRIVSDYACEVLARKANLKIDLLLEHLETEDENVRFQ
                     VIKTIGQIGGLAFNPIIKILQNGKKEERLFLLGVLQKINPNDKLIEALVQLLGDPGWP
                     IRNAAANCLRSYGEKAVSAVVRVLNSSNDDVQFWSRRVLLSMGSAAVIVLTQILRENS
                     DPAMTPHIISALLSMNNTEAVPAVLAFLENSDDSRVKEVMGGIPEISSREVVDTILNL
                     INHPVDRVVYWLAEILKKVSKPNLRRIVLLGLNHSEDRSRHFVLEAISGWKNVSDPEL
                     KTVVRQLEVEKSPRNILDIARVLGRNPSGALIEPLKTFLGNCEPDLMLDFMLSLMESE
                     RPGYEGVLAETLKTRSHGIKMDDVEKVGRILGLVYKSKTEGTIT"
BASE COUNT          281 a          271 c          308 g          276 t
ORIGIN      
        1 gatgacaagc ctcggagaca cccagcgcat cgtttcggac tacgcctgcg aggttctggc
       61 acggaaggcc aatttaaaga tcgatctgct gctggaacac ctcgagacag aagatgagaa
      121 tgtcaggttt caagttatca agaccattgg ccagattggt gggttggctt ttaatcctat
      181 tatcaaaata ttgcaaaacg gcaagaaaga agaacgtctg ttcctgcttg gcgtcttaca
      241 gaagatcaat ccaaatgaca agctgatcga agcgctggtt caattgctgg gtgatcctgg
      301 gtggccgatt cgcaatgccg ccgccaattg cctgcgatca tacggagaga aagctgtctc
      361 ggcggtcgtc cgtgtcctca actcttcaaa cgacgatgtg cagttttggt cgcgacgagt
      421 gctgttatcc atgggatcgg ctgcggtgat cgtgctgacg cagattctga gagaaaattc
      481 agatcctgcg atgactcccc acatcatttc ggccctgctt tccatgaaca acaccgaagc
      541 tgttccggcg gtgctcgctt tcctcgagaa ctctgacgac tcgcgtgtca aagaggtcat
      601 gggcggcata ccagagattt cctcgcgtga agtcgttgat actatcttaa atttgataaa
      661 tcatccggtg gaccgtgttg tttattggct tgctgagatt ctgaaaaaag tcagtaagcc
      721 gaatttgaga cgcattgtcc tcctcggtct gaaccactct gaggatcgca gtcgacattt
      781 cgtcctcgaa gcgatctctg ggtggaagaa tgtgagtgat cccgaactga aaacggtggt
      841 gcgacaactt gaggtcgaga agtctcctcg aaacattctg gacatcgctc gagtgcttgg
      901 tcgcaacccg agcggggctc tgatcgagcc actgaagaca tttctgggaa actgtgagcc
      961 agacttgatg ctggatttca tgctcagcct catggaatca gaacggccgg ggtatgaagg
     1021 ggtgctggcg gaaactctca agactcgttc tcatggcatc aaaatggatg atgttgagaa
     1081 agtcggccgc attctcgggc tcgtctacaa gagcaaaact gaaggaacca ttaccg
//
LOCUS       DAORHD010001652         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_186798_length_1134_cov_2.797034, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001652 DAORHD010000000
VERSION     DAORHD010001652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186798_length_1134_cov_2.797034"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PKO06_24220"
     CDS             <1..507
                     /locus_tag="PKO06_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72836.1"
                     /translation="KAYYGDHLNDDNMNQTITKVANGANSVNDLGVIMNEGMSHLNKR
                     FDELNKYQSEFLETHKAYQQKYAVDPSATTARSTQSPSSSVRPVAQSSGTTVRVQSPY
                     NGTSPTGTQGSYQISVAKAQENYQKAYRDYTQVLSNPSASATAREQAIENLRQARQLL
                     DLAKSSSK"
     gene            608..>1134
                     /locus_tag="PKO06_24225"
     CDS             608..>1134
                     /locus_tag="PKO06_24225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72837.1"
                     /translation="MTVLEHVLRQEIKPALGCTEPVAIAIAARHASLQAGGTVEALEI
                     TLSTNLFKNALEVGIPGTGGERGIPLAAALGCVIPLQTPTLTILEHVTPELLQQARHL
                     VERRAIKLSLAHDRRGVFIDARARGHSIGRAVIEGTHEHLVHLSRDGVVLLEESAPAV
                     HATTSETSSRSTLASL"
BASE COUNT          262 a          344 c          307 g          221 t
ORIGIN      
        1 aaggcctatt acggcgatca cctcaacgac gacaacatga accagacgat cacgaaggtc
       61 gcgaatggcg ctaactcggt gaacgatctg ggtgtgatca tgaatgaagg aatgtcacat
      121 ctgaacaagc ggttcgacga gttgaacaag tatcagagcg agttcctcga aacgcacaaa
      181 gcgtatcagc agaagtatgc cgtcgacccc agtgcgacca cagcgcgcag cacgcagagc
      241 ccctcttcgt cggtgcgacc tgttgcgcaa agttcaggca cgaccgttcg tgtacagtcg
      301 ccctacaacg gcacctcgcc aacgggcacc cagggttcat atcagatctc cgtggcaaaa
      361 gcccaggaaa actaccagaa ggcttaccgg gactatactc aggtcctcag caatccttcg
      421 gcatcagcca ccgctcggga acaagcgatc gagaatctgc gccaagcccg acagctgctg
      481 gatctggcca agagtagttc gaagtaagag cgcgtaaccg tccgccttca gagggcgcca
      541 gttgcagaac tggcgccctt tttcatgcct gtgatatact gctcaaaatc attggccgag
      601 gattttcatg accgttctcg aacatgtttt acgtcaggaa atcaaacccg cgcttgggtg
      661 caccgagccc gtggcaattg cgattgcagc gcgtcatgct tctctgcagg ctggtggcac
      721 cgtggaagct cttgagatca cgctttccac caacctgttc aagaacgctc tggaagtagg
      781 catccccgga accggcggcg aacgtggcat tcctctggcc gcggccctgg gctgcgtgat
      841 tccactgcag accccaacct tgaccattct cgagcacgtc acccccgaac tgctgcaaca
      901 ggcacgccat ctggtcgaac gtcgggcgat caagctgtcg ctggctcacg atcggcgtgg
      961 cgtattcatc gacgcccgtg ctcgcggcca cagtataggt cgggcggtca ttgagggaac
     1021 tcacgaacat ctggtacatc tgtcgcgaga cggagtcgtt cttctggaag agtcagctcc
     1081 cgctgtccat gccacgacgt cagagacttc cagtcgctcc acgctggcaa gcct
//
LOCUS       DAORHD010001653         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_187084_length_1133_cov_2.339518, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001653 DAORHD010000000
VERSION     DAORHD010001653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187084_length_1133_cov_2.339518"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1133)
                     /locus_tag="PKO06_24230"
     CDS             complement(<1..>1133)
                     /locus_tag="PKO06_24230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72838.1"
                     /translation="YTTRQALTESERQYRALADLAHDMIFMVSPEFRVLYVNSFAAQH
                     LRATPEQLAGHHLSELFPKDNAERMIENLKQVFATGTARYIEDRMIFPGREIWLDSRL
                     VPLHDEQGKIYAIYGLSRDVSARRAAEEATRTSEERFRMLFERASEAIFVYNQRGLIE
                     EANPRATELSGFPHEQLIGMNPIDFAPMEQRPALAQVFQRLQTEREVTVELQQFRKDG
                     QARDVSATVSLLPDGRYLGMVRDITEQRRLEDELMRSQKLESLGVLAGGIAHDFNNIM
                     TVVFGHLSLSRLLLKTGADIDGKLVEAEKAALRARDLTQQLMSFSKAGTPSKKPTSLT
                     ELLTESSSFVLHGTPFTCEYQIPQNLWAIDADDDSSEPREFDD"
BASE COUNT          252 a          300 c          320 g          261 t
ORIGIN      
        1 tgtcatcaaa ctcgcgaggt tcggacgagt cgtcgtcggc atcgatcgcc cacaggttct
       61 gagggatttg atattcacaa gtgaaaggcg ttccatgaag aacgaaactg gacgattccg
      121 tcagaagttc ggtcaaggat gtcggttttt tactgggcgt gccggccttc gaaaaggaca
      181 tcaactgctg cgtcagatcg cgtgcgcgca gggctgcctt ctccgcttcg acaagcttgc
      241 cgtcaatgtc cgctccggtc ttgaggagca gtctggagag gctcagatgg ccgaatacca
      301 cggtcatgat gttattgaaa tcatgcgcga tgccaccggc aagcacgccg agcgactcga
      361 gtttctgtga gcgcatcaat tcatcctcga gtcggcgttg ctcggtaatg tcgcgaacca
      421 tgcccagata tcgtccatca ggcaacagag agacggttgc ggaaacgtcc cgtgcctgcc
      481 catcctttct gaactgctgc aattcaaccg tgacctctcg ttcggtctgc aggcgctgaa
      541 acacctgtgc gagagcgggg cgttgttcca tgggcgcgaa gtcgatcggg ttcatgccga
      601 tcaactgctc gtgtgggaat ccggacaatt ccgtggcacg aggatttgct tcttcgatca
      661 gtccacgttg attatagacg aatatcgctt cagaagcccg ttcgaacaac atgcgaaatc
      721 gttcttcgct ggttcgcgta gcttcctctg cggcgcgacg ggccgagaca tcgcgtgaaa
      781 gaccgtagat ggcgtagatc ttcccctgtt cgtcatgcag gggaaccagt cgagaatcaa
      841 gccagatttc acggccggga aaaatcatcc gatcttcgat gtatcgagcc gtaccagtcg
      901 caaaaacctg tttcaggttc tcgatcatgc gttcggcatt gtctttggga aaaagctcgc
      961 tgagatgatg ccccgctaac tgttccggtg tcgcgcgcag atgctgcgcg gcaaatgaat
     1021 tgacatacag aacgcgaaat tcgggcgaga ccatgaaaat catgtcgtga gccagatcgg
     1081 ccagagcgcg atactggcgc tcgctttccg ttaaagcctg acgagttgta tag
//
LOCUS       DAORHD010001654         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_187106_length_1133_cov_2.205937, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001654 DAORHD010000000
VERSION     DAORHD010001654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187106_length_1133_cov_2.205937"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="PKO06_24235"
     CDS             complement(<1..657)
                     /locus_tag="PKO06_24235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72839.1"
                     /translation="MPHQCLVLILLICAVTCCPLAAWERDVDLRDGTRLYKGAAIYGW
                     HQKLLGDIDVLGLKVDLSGDAAFDDRVGMGASFFLPVTRRTTLLGGYDEFDHSGKVTK
                     TVNFRRKQYRPGARIDLQHEWLDLTGASRVRQWEGGFGDFLYGMKVCRSELNLRGNDP
                     ITNAFQKAAWDTMFPIPYLGFGGGARVTSHMLILGRFKAIVLPSTGEDLFTSYDAELT
                     G"
     gene            complement(843..>1133)
                     /locus_tag="PKO06_24240"
     CDS             complement(843..>1133)
                     /locus_tag="PKO06_24240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV72840.1"
                     /translation="GVLQHHERVDGKGYPDGLKDEEIPVGSRIIMVSDTFDAITSHRP
                     YRKPLTFAEAFAEVQRCRGTQFDPLVVDVFVNLRSTPYCPDWLRKDALGEND"
BASE COUNT          251 a          328 c          335 g          219 t
ORIGIN      
        1 cccggtaagt tcggcgtcat acgaggtgaa aaggtcttcg ccggtcgagg gaagcacgat
       61 cgccttgaat cggcccagga tcagcatatg cgacgtgacc cgcgctccgc caccaaatcc
      121 cagataggga atcgggaaca tcgtatccca ggcggctttc tgaaaggcat tggtgatcgg
      181 gtcgttgccg cgtagattca gttcgctgcg acaaactttc atgccgtaga gaaagtcgcc
      241 gaagccgcct tcccattggc gcacccggct ggcgccggtc agatcgagcc attcgtgctg
      301 tagatcaatg cgcgcacccg gcctgtactg cttgcggcga aagttgaccg tcttcgtaac
      361 tttgccggaa tgatcgaact cgtcgtagcc gcccagcaga gtcgtgcggc gtgtcaccgg
      421 cagaaagaaa gacgccccca ttccgacgcg atcgtcgaat gccgcatcac ccgagaggtc
      481 caccttcaga ccaagaacgt cgatgtcgcc caacagcttc tgatgccaac catagatcgc
      541 cgcgcctttg tacagccgcg tgccgtcgcg cagatcgaca tcgcgttccc aggccgccag
      601 cgggcaacac gttaccgcgc agatcaggag aatcagcacc aggcattgat gaggcatcaa
      661 tcgaaacata atattgatgc ccgctctatc ggccgaaagt caaagtttag gtatgggtta
      721 ccgtaatagc acgcgaaaat cagccgagcg tgtgacggtc gtttcgggct ccccgtcgtc
      781 agaccgctga tctcactcca gaatcggaca attctccgcg aaagattggg aaaaaagtgg
      841 ggtcagtcgt tttcgccgag ggcgtctttg cgcagccagt cggggcagta gggggtgctg
      901 cgcaggttca cgaagacatc gaccacgagg gggtcgaact gtgtgccgcg gcagcgctgc
      961 acttcggcga aggcttcggc gaaggtcagg ggcttgcgat aggggcgatg cgaggtaatc
     1021 gcatcgaacg tgtcagaaac catgatgata cgcgagccaa cgggaatctc ttcgtctttg
     1081 agaccgtcgg gatacccctt tccatccacg cgttcatggt gctgaagcac gcc
//
LOCUS       DAORHD010001655         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_187523_length_1131_cov_3.762082, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001655 DAORHD010000000
VERSION     DAORHD010001655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187523_length_1131_cov_3.762082"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..>1131
                     /locus_tag="PKO06_24245"
     CDS             158..>1131
                     /locus_tag="PKO06_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72841.1"
                     /translation="MIDDKRTRRLVPFPMQAVLLIFFPLALLVVIGWYGIRLAEEQHE
                     RESLRDLTRRLQQLGQTCAPSFYFAREWGTLFGELQRSAHLYDQQVKLVRDWSRRQNL
                     EIEVYRLSAAGEVRVLTPELTRAQAIIKDFWNFLRTGKEEYRYHFYAPPAVRALFGRV
                     FAYDECFAHRGEVLEISHAGKTGFLYWNSSENPDGGGVIIVAWAVPGLPSLLDRFRDH
                     ILGSDTALVVQPADETLFLRQPPTNALAAGTEVERYAWSSFRIDDVRVYAGIPRTRLR
                     LSLERQLLAGFAATVGVLLLILLWRWNRGEQARFLSIRWKLAGPFLYAL"
BASE COUNT          216 a          301 c          353 g          261 t
ORIGIN      
        1 ccaagctcac gaatgttctg atttctccaa gaacgcgaga gaagttggga gcctttgccg
       61 gggtggcatt tctggatgaa gtggtcgtca agggaaaaga aaagcccgtg gcattgtacg
      121 aattgacagg tctggaacca ttcacggaga gcatggtgtg atcgacgata aacgaacgag
      181 acgattggtg cccttcccca tgcaggccgt cctgctgatc ttcttcccgt tggctctgct
      241 cgtggttatc ggctggtacg gcattcgtct ggccgaagaa cagcatgaac gtgaatcatt
      301 acgtgatctg actcgccggt tgcaacaact gggacaaacc tgtgcgccgt cgttctattt
      361 cgcgcgagag tggggaacat tgttcggcga actgcagcgg tcggcgcatc tgtacgatca
      421 gcaagtaaaa ctggtgcgcg actggagccg gcgacagaac cttgaaatcg aggtctatcg
      481 actctctgct gccggcgagg tgcgggtgct gaccccggaa ctcacgcggg cgcaggcgat
      541 catcaaggat ttctggaact ttctgcgcac cgggaaagag gaatacaggt atcacttcta
      601 cgctccgccg gccgttcgag cgctttttgg acgggtattc gcctacgacg agtgtttcgc
      661 ccatcgcggc gaggttctcg agatctctca cgcgggcaag acgggattcc tgtattggaa
      721 ctcgtctgaa aacccggacg gagggggagt gatcattgtg gcgtgggccg ttccgggcct
      781 gccttctctg cttgaccggt ttcgtgatca cattctcggg tcggatacgg ctctggtggt
      841 gcagccggcg gacgaaacgc tcttcttgag acaaccaccc acgaacgctc tcgcggcggg
      901 aaccgaggtc gagaggtatg cctggagctc atttcggatc gatgacgtcc gggtctatgc
      961 agggattcct cgaacgcgcc tgcgactttc gcttgaacgg cagctccttg ccggcttcgc
     1021 cgcgaccgtg ggggttctgc tgctgatcct tctctggcgc tggaatcgag gcgaacaggc
     1081 caggtttctt tccattcgct ggaagttggc gggtccgttt ctgtatgcgc t
//
LOCUS       DAORHD010001656         1131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_187591_length_1131_cov_2.758364, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001656 DAORHD010000000
VERSION     DAORHD010001656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187591_length_1131_cov_2.758364"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1131
                     /locus_tag="PKO06_24250"
     CDS             <1..>1131
                     /locus_tag="PKO06_24250"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNV72842.1"
                     /translation="ASSPPPRRDPTYDHRIGFPAETALLLRLHQDHGTLALPQIMAPA
                     IRAAQTGFIVTGYLETQIAKGLKKFSDPEARALFAPNGRPIRAGERLRQTALARTLMR
                     IAADHGESFYRGDDAHIMVRDLQARGAKFVTDDFASFRPRPMVPLTLEVGAYRLIGTP
                     PPSSAIVALALIREWFRRRWNLDDPADAIDHLRVVRALINLKHSRLASCWQKPLRFLE
                     LADDAVRQTTMQLDDENALSTSEDEPPEHTTHLVVWDKTGMIVTMTLTLGTHWGTKDF
                     CPLGFFYNSEGLLFRHAGHTFPADYPTTVGPISAKSPVLVLKDRKPLMAIGGAGSNRI
                     VANVGTIAGAVLFGGHRLEEALHAPRRMPDPQKLYLEWGTPDT"
BASE COUNT          215 a          348 c          326 g          242 t
ORIGIN      
        1 tgcctccagt ccgccgccgc ggcgagaccc gacctatgac catcgcatcg gtttccctgc
       61 cgaaaccgcg ctgttgttac gactgcacca ggatcatgga accctggctc tgccgcagat
      121 catggctcct gctattcgcg ctgcccagac tggtttcatc gtcaccggtt acctggaaac
      181 acagatcgcc aaagggttga agaagttctc tgaccccgag gcccgtgcgc tcttcgcccc
      241 gaacgggcgc ccgattcgag cgggtgagcg tttgcggcag acggcgctgg ccaggaccct
      301 gatgaggatt gccgctgatc atggggaatc gttctatcga ggtgatgatg cccacatcat
      361 ggttcgtgat ctgcaagctc gtggagcgaa gttcgtcacg gatgacttcg cgtcctttcg
      421 tccacgcccc atggtgccgc tcaccctcga ggttggtgcg tatcgcctga ttggcacacc
      481 ccctccgtcg tcggcgatcg ttgcgctcgc tttgatccgt gaatggttcc gccggcgttg
      541 gaatctcgac gacccggccg atgccatcga tcatctgcgc gtagtccgcg ccctgatcaa
      601 tctgaaacac tcccggctgg catcgtgttg gcagaaacct ctgcgatttc ttgaactggc
      661 cgatgatgcc gttcggcaga cgacgatgca gctagacgat gaaaatgccc tatcgaccag
      721 tgaagatgag ccgccggagc atacgacgca tctggtcgtc tgggacaaaa caggaatgat
      781 tgtgaccatg accttgaccc tgggcacgca ctgggggaca aaggacttct gtccgctcgg
      841 attcttttac aatagtgaag gcctgttgtt tcgccatgcc ggccacacct tccccgccga
      901 ttacccgacc accgtcgggc ccatctcggc caagtctccg gtactggttc tgaaggaccg
      961 aaaaccactg atggctatcg gaggagccgg cagcaatcgc attgtggcca atgtggggac
     1021 cattgccgga gccgtgctct ttggtgggca tcgtctcgaa gaagcgctgc atgcgccacg
     1081 tcggatgcct gacccgcaga agctgtatct cgaatgggga acgcctgata c
//
LOCUS       DAORHD010001657         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_187811_length_1130_cov_5.697674, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001657 DAORHD010000000
VERSION     DAORHD010001657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187811_length_1130_cov_5.697674"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PKO06_24255"
     CDS             complement(<1..648)
                     /locus_tag="PKO06_24255"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV72843.1"
                     /translation="MKHLFYACLILNLLGIGGAMAQEKTFSWDPLVIEKLDNGITVIV
                     KEDRARPLAVTDVWFKVGSRNEVERTNGIAHFLEHTLFKGTKKRGIGEIARDIEAMGG
                     RTNAATSLDFTHYYIECEADKIMQALEIHADVFRNSLLDPKAIDDERGVIIEEIKRGE
                     DTPGSVLWNTLTANVFSSHPYRRPIIGPRENIASGGITRDDMHEFFTTWYNTGNMY"
     gene            complement(697..>1130)
                     /locus_tag="PKO06_24260"
     CDS             complement(697..>1130)
                     /locus_tag="PKO06_24260"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV72844.1"
                     /translation="FDELRAFRQLADELWPGRFPPAEILKLVTTSPAAIFRVAKQCGS
                     LEAGKLANFLVFPDTQQDPWASFFELTAKSLSLVVHRGALAYGDERFRSVCSVRFDHY
                     SEVLVEGVPKLIWGKPIQLLERIEHKLGESRRFPFLPVNEL"
BASE COUNT          253 a          332 c          301 g          244 t
ORIGIN      
        1 atacatgttt cccgtgttgt accaggtcgt gaaaaactca tgcatgtcgt cacgcgtgat
       61 tccgccagac gcaatgttct cacgcggccc gataatcggt cgacggtacg ggtgactgct
      121 gaaaacattc gccgtcagag tgttccagag aacgctgccc ggggtatcct cgccgcgttt
      181 gatctcttca atgatgactc cgcgctcgtc gtcgatcgct ttcggatcga gcagagagtt
      241 gcgaaacaca tcggcgtgaa tctcgagcgc ctgcatgatc ttgtccgctt cacactcgat
      301 atagtagtgc gtgaaatcaa gagaggtcgc ggcattggtt cggccaccca tcgcttcgat
      361 atcgcgcgcg atttcgccaa taccacgctt cttggttccc ttgaacagcg tatgttcgag
      421 gaagtgggca atgccgttgg tgcgttccac ctcattacga ctgcccactt tgaaccagac
      481 gtccgtgacc gcaagcggtc tggctcggtc ttccttgacg atgacggtga taccattatc
      541 gagtttctcg atgacgagcg gatcccacga aaatgtcttc tcttgagcca tggcccctcc
      601 gattccaagc agattgagga tcaagcaggc atagaacagg tgtttcatca gtgaacctcc
      661 aaacagagta ccagatacga tagaggaatt acgtctctac aactcgttga ccggcaggaa
      721 cggaaatcgt cgcgattcgc cgagtttgtg ctcgatgcgc tccagcaact gaatcggctt
      781 tccccagatc agcttgggaa cgccttccac aagcacttcg gagtaatgat cgaaacgcac
      841 cgagcagaca ctgcgaaagc gttcgtctcc gtatgccagg gctccccggt gcacgaccag
      901 agacagcgac ttcgccgtca gttcgaaaaa actggcccac ggatcctgct gggtgtcggg
      961 gaagaccagg aaattcgcga gtttccccgc ctcgagcgac ccacactgct ttgccacccg
     1021 aaagatcgcc gccggcgagg tcgtcaccag cttcagaatc tctgccggcg ggaagcgtcc
     1081 gggccacaac tcgtcggcca gctgacgaaa cgcgcgcagc tcatcgaaga
//
LOCUS       DAORHD010001658         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_188687_length_1127_cov_3.837687, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001658 DAORHD010000000
VERSION     DAORHD010001658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188687_length_1127_cov_3.837687"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PKO06_24265"
     CDS             complement(<1..322)
                     /locus_tag="PKO06_24265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV72845.1"
                     /translation="MKERKAKADKRRQILEAACSCFAQNGFAATRLDDIAEKAKVAKG
                     TIYLHFENKDSLYLALFRETLDELFDTIDSAVMAVDSFAEKAQCAIRLYLEFFEHNRE
                     KFSIF"
     gene            complement(468..1061)
                     /locus_tag="PKO06_24270"
     CDS             complement(468..1061)
                     /locus_tag="PKO06_24270"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV72846.1"
                     /translation="MPEVVQQIWNRASVKRSPAEWALRWVWDHPEVTVVLSGMTLDEH
                     LEENLRIADQAKPQSLSAEERQVVADAAAAYRRLMQIACTGCQYCMPCPAGVNIPTCF
                     EVYNTHALFQDKQASMMYLVRLAGLLGTPAQASKCTSCGACLKKCPQNLPIPTLLKQV
                     ANTFEGPMSSIKLFAFRSFLKLERFWSMFRHRFLHRR"
BASE COUNT          243 a          288 c          359 g          237 t
ORIGIN      
        1 gaaagatcga gaacttctcg cgattgtgct cgaagaactc gagatacagg cgaatggcgc
       61 attgagcctt ctcggcaaaa ctgtcgactg ccatgaccgc tgaatcgatc gtgtcgaaca
      121 gttcatcgag ggtctcgcga aacagggcga gatacaggct gtctttgttt tcgaagtgca
      181 gatagattgt gcccttggcc acttttgcct tttcggcgat gtcatcgagg cgggtggcgg
      241 caaacccgtt ctgcgcgaaa cacgaacagg cagcttcgag aatctggcgg cgtttgtctg
      301 ccttggcttt gcgttctttc atgaagaggc cccgttcgga aaatggtcat caagaaaact
      361 gaccggtcag tcattaatta tgataacaga gagtttcgca aaaagcaagg gcgcagagca
      421 acggctgccg tgtgcggtgg tcgttggtcg tgagggggga tgaatggcta gcggcgatgc
      481 agaaagcggt gtcgaaacat ggaccagaaa cgttccagct tcaggaagct gcgaaaggcg
      541 aagagtttga tcgagctcat ggggccttcg aacgtgttgg ccacctgctt gagcagggtg
      601 ggaatcggca gattctgcgg gcacttcttg agacaggcgc cacaggaggt gcacttcgat
      661 gcttgcgccg gggtgccgag cagtccggcg agcctgacca gatacatcat cgaggcttgc
      721 ttgtcctgaa agagcgcatg ggtgttatag acttcgaaac aggtgggaat attgaccccg
      781 gcgggacagg gcatacagta ctggcagcca gtgcaggcga tctgcatcag acgccgatag
      841 gcggctgccg catccgcgac gacctgtcgc tcttcggcgg aaagtgattg cggtttggcc
      901 tggtcggcaa tgcgcaggtt ctcttcgaga tgctcatcga gcgtcattcc tgagagcacc
      961 accgtgactt cagggtgatc ccagacccag cgcagagccc attctgccgg gctgcgcttc
     1021 accgacgccc ggttccagat ctgctgaacg acttcaggca cgcgtccggc gaggttgaag
     1081 cgcagcccgg cagaatgggc tctgcgctgg gtctgggatc accctga
//
LOCUS       DAORHD010001659         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_188962_length_1126_cov_3.659197, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001659 DAORHD010000000
VERSION     DAORHD010001659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188962_length_1126_cov_3.659197"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKO06_24275"
     CDS             complement(<1..506)
                     /locus_tag="PKO06_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72847.1"
                     /translation="MIRLFLAGLIGSLASGLVAGWLRYWLGFMVIFHGLAFALLATWV
                     FNRLRPTPTPDLLWTNRAAWRSAPVFLLLFWVGQMIGLGLAQPVFDPWQFFWQILAGS
                     EGESFVAMTQHRTYSGGLTGLFWAFTNLLDSGLMLIFFATMMRDDEEPETSTETEAEG
                     DDDDADAV"
     gene            complement(756..>1126)
                     /locus_tag="PKO06_24280"
     CDS             complement(756..>1126)
                     /locus_tag="PKO06_24280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNV72848.1"
                     /translation="IATQEVIEELIALARSMSAATKRGEDLGLNPDEVAFYDALAANE
                     SAVKAMGNAELKVIAAELVTQVRKSVTIDWTMRESARARIKVMVKRILKKYGYPPDLQ
                     EEATRTVLAQAELLCADWVS"
BASE COUNT          224 a          354 c          333 g          215 t
ORIGIN      
        1 aacacggcat ccgcgtcgtc gtcgtcgccc tctgcttcgg tttcggtcga ggtctcgggt
       61 tcttcgtcgt cacgcatcat ggttgcgaaa aagatcagca tgaggcccga atcgagcagg
      121 ttggtgaagg cccagaaaag gccggtgagg ccgccactgt aggttcggtg ctgcgtcatg
      181 gcgacgaagc tttcgccctc gctgccggcc aggatttgcc agaagaactg ccacggatcg
      241 aaaaccggct gtgccagtcc cagcccgatc atctgcccca cccagaacaa cagcaggaaa
      301 accggcgccg accgccaggc cgcgcggttg gtccagagca ggtcaggggt cggggtcggg
      361 cgaagtcggt tgaagaccca ggtggccagc aacgcaaacg ccagcccgtg aaaaatgacc
      421 atgaatccga gccagtagcg cagccacccg gccacgaggc ccgaagcgag actgccgatc
      481 aatcctgcca gaaagagcct gatcatgcct ctccccctcg ttcggtgttc gtcgttgcca
      541 gcttaccaca ggcacgtgtc atctgcacgg ttctgagtca ggccgacagg atcgtttcag
      601 tctcccatct ggattttccc cttggcatcg ctcaacgatt cgctcacacc aagaggagaa
      661 cgaaacagga tggaatgtcc atgctgcggg caatcgggaa acttttctgc gtgcgacagg
      721 cttttggtct cacccgcgcc aagacgccgg cggggtcagg aaacccagtc ggcgcagagc
      781 agttcggcct gcgcgagaac cgtccgagtc gcttcctcct gtaaatcagg cgggtagccg
      841 tatttcttga gaatgcgctt caccatgacc ttgatacggg cacgcgcact ttcgcgcatg
      901 gtccagtcga tggtcacgct cttgcgcacc tgggtcacca gttccgccgc gatgactttc
      961 agctcggcgt tgcccatggc cttgacggcg ctctcgttgg cggccagcgc atcatagaaa
     1021 gcgacctcat cagggttcaa cccgagatcc tcgccgcgtt tcgtcgcggc actcatcgac
     1081 cgggcgagag cgatcagctc ctcgatgact tcctgagtgg cgatgg
//
LOCUS       DAORHD010001660         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_189222_length_1125_cov_4.140187, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001660 DAORHD010000000
VERSION     DAORHD010001660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189222_length_1125_cov_4.140187"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1125)
                     /locus_tag="PKO06_24285"
     CDS             complement(<1..>1125)
                     /locus_tag="PKO06_24285"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNV72849.1"
                     /translation="ELSLHDFIHIIRKRYLVFLLTVSLVTIGGVLITLNLQPIYQSKA
                     TIMIERPPITSEWQLYTEEKDFKMVMQLMNSQSLLQEAMEQAGIASSAMRDMRLNAKM
                     IPDSQMIDITIEDVLPRRLVTVFRHFLPIVTDRIAKTESQNLVRKVDFIKMQLKVVEE
                     RLAATDKILQGYAEREMDVSVSDKMGKINADIAKIQDERRMSEVDLSVIGDVLTDLQK
                     KALSVTRGTRSWDTTLQELVELKTTMLQLQRERIKLTSLYQPSHPDVKSLDDQIDFVS
                     RQIEEKSTSVATSSLILDSSGQTGPYVFQKLLDLEAQQKTLVLKIAQMDTLLAEKTAV
                     FKSLLARLEEFNSLSRRQKVDEQIHLSLIEKLNQAEVDRGT"
BASE COUNT          229 a          334 c          283 g          279 t
ORIGIN      
        1 ggtgccccga tcgacttcgg cctggttcag tttttcgatg agactgagat gaatctgctc
       61 atcaactttc tgacggcgcg acaggctgtt gaactcttcc agacgcgcca gcagagactt
      121 gaacacggct gtcttctccg ccagcagtgt atccatctgc gcgatcttga gaaccagcgt
      181 cttctgctgc gcttccaggt ccagcagctt ctgaaagaca tatggccccg tctgtcctga
      241 tgaatccagt atcagactac tcgtcgccac ggacgttgac ttctcttcga tctgtcgact
      301 gacaaaatcg atctggtcat ccaggctctt cacatccggg tgcgaaggct ggtacaggct
      361 ggtcaacttg atccgttcgc gttgcaactg cagcatggtg gtcttgagtt cgacgagttc
      421 ctgcagcgtc gtatcccacg accgggttcc ccgggtcacc gacaacgcct ttttctgcag
      481 gtccgtcaac acgtcgccaa tgacactcag atcgacttct gacatgcgcc gctcatcctg
      541 aatcttcgcg atgtcggcgt tgatcttccc catcttgtcc gagaccgaga catccatttc
      601 ccgttccgcg tatccctgca ggatcttgtc cgtcgcggcc aggcgttcct cgaccacttt
      661 caactgcatc ttgatgaagt cgaccttgcg caccagattc tgactttcgg ttttcgcgat
      721 gcgatcggtg acaatcggca gaaagtggcg aaacaccgtg accagacgcc gcggcagcac
      781 gtcttcaata gtgatgtcga tcatctgact gtctggaatc atcttcgcgt tcaggcgcat
      841 gtcacgcatg gcgctcgagg cgatgcccgc ctgttccatc gcttcctgca acagcgactg
      901 agagttcatc agctgcatca ccatcttgaa atctttctct tcggtataca gctgccactc
      961 gctggtgatc ggagggcgct cgatcatgat cgtcgccttc gactgataga tcggctgtag
     1021 attcagggtg atcagcacgc cgccaatcgt gaccagactg accgtcaaca gaaaaacgag
     1081 ataccgcttg cgtatgatgt gaatgaaatc atgcagggaa agctc
//
LOCUS       DAORHD010001661         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_189235_length_1125_cov_3.650467, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001661 DAORHD010000000
VERSION     DAORHD010001661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189235_length_1125_cov_3.650467"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1125)
                     /locus_tag="PKO06_24290"
     CDS             complement(<1..>1125)
                     /locus_tag="PKO06_24290"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV72850.1"
                     /translation="KTLGQTTTMAMRPGKVLWDPLQKKIRELTEKKKPAAPVKKGPRK
                     RLGDIIVESKYATEEQMKEAIEGAKKEKLRLGAYLVKKGTLSNKQLSIALSKQFDIPY
                     IDLEESLVDPVLATLLPKKLCYEHILCPVKKEENKIVIAMTDPTDIITIDHIEMMTGM
                     RLSPVVSSELAIVSALDKLYGETADALADQVGNKEESNDLPDELAGDLNENAAPIIKL
                     VNLVLTQAAQTGTSDIHIEPFEHEVKIRFRKDGMMKMFMTPNKAAHAAIVSRIKVMSN
                     LDIAETRLPQDGRIKMTLGSRRVDLRVSCVPCVWGEKVCMRLLDQGNLKVNLNDLGFE
                     PRALDNFMEGVKSPNGIVLVTGPTGSGKTTTLYSCLHLLN"
BASE COUNT          217 a          318 c          291 g          299 t
ORIGIN      
        1 ttgttgagca gatgcagaca ggaatacagc gtcgtggttt ttccggaccc ggtcggcccc
       61 gtcaccagta cgatcccgtt cggcgacttc acgccttcca tgaaattgtc gagtgctcgt
      121 ggttcgaacc ccaggtcatt cagattgacc ttcaggttgc cctgatcgag cagacgcata
      181 cagactttct caccccagac acagggaaca cacgaaacgc gcagatcgac ccgccgggac
      241 ccgagggtca tcttgatgcg gccatcctgc ggcagacggg tttccgcgat gtcgaggttc
      301 gacataacct tgatacgact gacaatcgca gcatgcgccg ccttgttcgg ggtcatgaac
      361 atcttcatca tgccgtcttt acgaaagcga atcttgactt catgttcaaa cggttcgatg
      421 tgaatgtccg aggtaccggt ctgggctgcc tgcgtcagga caaggtttac cagcttgatg
      481 atcggagcgg cattctcatt cagatcaccg gccaactcat caggcaggtc gttgctttct
      541 tctttgttgc ccacctgatc ggccagagca tcggcggttt ctccgtacaa cttatcgaga
      601 gctgaaacaa tagccaattc gctgctgacg accggtgaaa gtctcatgcc cgtcatcatc
      661 tcgatgtgat cgatcgtgat aatgtcggtc ggatcggtca tcgcgatgac gattttgttc
      721 tcttcctttt tgaccgggca gagaatgtgc tcatagcaca gtttcttcgg cagcagagtc
      781 gccagtaccg gatcgacaag actttcttcg aggtcgatgt agggaatatc gaactgtttc
      841 gacaacgcga tcgacaactg cttgttcgac agcgtccctt tcttgaccag ataggctccg
      901 agtcgcagct tttctttctt ggcaccctcg atcgcctctt tcatctgttc ttcggtggcg
      961 tatttcgact cgacgatgat atcgcccagt cgcttgcgcg gccccttctt gaccggtgct
     1021 gccggttttt tcttttcggt cagctcacgg atttttttct gcagcgggtc ccagaggacc
     1081 tttccggggc gcatggccat cgtggtcgtc tgacccagcg ttttc
//
LOCUS       DAORHD010001662         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_189259_length_1125_cov_3.271963, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001662 DAORHD010000000
VERSION     DAORHD010001662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189259_length_1125_cov_3.271963"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>1125
                     /locus_tag="PKO06_24295"
     CDS             271..>1125
                     /locus_tag="PKO06_24295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV72851.1"
                     /translation="MRFLYSALIFLASLASLLGFLLFPPVVVLAMAQRRGTCSRLPVV
                     LGFCIAVVCHCGLEYEANTFFRDDSRPMMALYRWTSLGTWLVRRDDFWYDAAERFACD
                     TPMTFLLTHGASPTEGLRQILRQLSLYGNTHATPFVPPLLSSGADPNVLIFGQSALAL
                     TIDRGKFDLATLLLKAGATPSPATDQWGKTLLHRSAAAGAEPEFLLLLADKMGSGSWN
                     LLDARGRAPIHELSTVIQLTLLASMPETLLTTGRDGGTLIHYAIEQGRERLIPRLLEL
                     GFTWQTPDS"
BASE COUNT          207 a          349 c          301 g          268 t
ORIGIN      
        1 tcgccgggtg aaaacccttc ttgttcagat gctccgccac gcccagaacc ttcatggggc
       61 atcgcgcgat acaaatgccg caacccttgc acaggtcttc attaatcagc cacttcgaca
      121 cggcaatcat tcctcctgga ggatgtttga atggccaatc ataccgcaaa acagcggctt
      181 tcacaacgaa cgagacgcga gattatcccg gcgtttttcg cgtgaggtat aatcgtcgta
      241 tcatctcggc acggcacgtg agaggcagtc atgcgctttc tgtattccgc tctgattttt
      301 cttgccagcc tggcttcact gctcgggttt ctgctgtttc cccccgtggt ggttctggcg
      361 atggcccagc gtcgcggcac gtgttcgaga cttcctgtgg tcttgggctt ctgcattgcc
      421 gttgtctgtc attgcgggct tgagtatgag gccaacacgt tttttcgcga cgacagccgc
      481 cccatgatgg ctctctatcg ctggacttcg ctggggacct ggctcgtcag gcgggacgac
      541 ttctggtatg acgccgcgga gcgcttcgcg tgcgatacac ccatgacgtt tctcctgacg
      601 cacggcgctt cgcccacaga aggtttgcgc cagattctgc gacaactcag tctctacggt
      661 aacacccatg ccaccccatt cgttccacct ctgctgagtt ccggagctga tcccaatgtt
      721 ctgatattcg ggcaatctgc tcttgcactg acgatcgatc gcgggaagtt cgacctcgcg
      781 accctgttgc tcaaagcggg agccaccccc tcgcccgcca cggaccaatg ggggaaaacg
      841 ctgctgcatc gctctgccgc agcgggcgcg gaacccgagt ttctgctcct gctggcggac
      901 aaaatgggct ctggttcctg gaatcttctc gacgcgcgag gacgagcccc catccatgaa
      961 ctgagcacag taattcagct cactctcttg gcatcgatgc ctgaaacctt gttgacgacg
     1021 ggaagagacg gcggcactct gatccactat gcgattgaac aaggtcgcga gcgtttgatc
     1081 ccgcggctgc tcgagctcgg attcacctgg cagactcctg attcc
//
LOCUS       DAORHD010001663         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_189363_length_1125_cov_2.229907, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001663 DAORHD010000000
VERSION     DAORHD010001663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189363_length_1125_cov_2.229907"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PKO06_24300"
     CDS             complement(<1..530)
                     /locus_tag="PKO06_24300"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72852.1"
                     /translation="MIIAPDEIIRKAQKALKEGKPQASALTAIGMAYFEKGLLDKAIF
                     HYQRALEADARFAPAYAGLGNVYAKKGKVTESVFNLREAIKLAPNCGLLYNWLGDAYF
                     DQGKLEDAIREYSRATELNTLDSNAHNDLADAYRLRGDWEQALSNYRKTLEIDPGDTN
                     AVLELTQVLLQLDRPA"
     gene            complement(574..>1125)
                     /locus_tag="PKO06_24305"
     CDS             complement(574..>1125)
                     /locus_tag="PKO06_24305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72853.1"
                     /translation="DDAHWGVVSAYADPLSTGIWPVFRRRSGFLGPALIPDAAALKRW
                     IETTSASDHQTSLDVAWFRRHRAELRSYLSRETDTAFRTRIKQDLERSLADCHGWHDG
                     DTHALVWPWGSTGPASDAAACDAGYNLAFLTATGANVPGTDPRHAYRFPVKKPDLFRF
                     ALGVWMRESPWLARLYGLLRGTV"
BASE COUNT          245 a          340 c          310 g          230 t
ORIGIN      
        1 tctgccggac gatcgagttg caacagcacc tgagtcagct cgagaacggc attcgtatca
       61 cccggatcaa tttcgagcgt tttccgatag tttgacaatg cctgctccca gtcgccccgc
      121 aggcggtagg catcggcgag atcattgtgc gcattcgaat cgagcgtgtt gagttcggtc
      181 gcccgcgaat actcacgaat ggcatcttcg agctttccct gatcgaaata ggcatctccg
      241 agccagttgt acagcaaacc acagttcggt gccagtttga tcgcctcgcg cagattgaac
      301 accgactcgg tgactttccc ttttttggca tacacgttac ccagaccagc ataggcaggg
      361 gcaaagcggg catcggcttc gagcgctcgt tgatagtgaa agatcgcctt gtcgagcagc
      421 cctttttcaa agtacgccat accgatcgcc gtcagggcgc tcgcctgcgg tttcccttcc
      481 ttgagggctt tctgagcctt gcgaatgatt tcgtcaggag caataatcat gctctgtcat
      541 tataccatag tgacaatgcg ttcttcagcc aactcacacc gtgccgcgca gcaagccata
      601 cagacgggcc aaccagggcg actcacgcat ccagactcca agagcgaagc gaaacaagtc
      661 cggctttttc accgggaaac gatacgcatg ccttggatcg gtccccggaa cgttcgctcc
      721 ggttgccgtg agaaatgcca gattgtaccc cgcgtcgcag gctgccgcgt ctgatgcagg
      781 ccccgttgag ccccacggcc agacgagcgc atgggtgtcg ccgtcatgcc agccgtggca
      841 gtctgcgagc gaccgctcga gatcctgctt gatccgggtt cgaaaagcgg tatcggtttc
      901 gcggctcaag tatgaccgaa gctcggcgcg atgacgacga aaccacgcca catcgaggct
      961 ggtctggtga tccgaagcgc tcgtcgtctc gatccaccgt tttaatgcag ccgcatcagg
     1021 aatcagagcc gggccgagaa accctgagcg acggcgaaac actggccaga tacccgtcga
     1081 caaaggatca gcatacgcgg agacgacacc ccagtgagcg tcgtc
//
LOCUS       DAORHD010001664         1124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_189498_length_1124_cov_3.588400, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001664 DAORHD010000000
VERSION     DAORHD010001664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_189498_length_1124_cov_3.588400"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PKO06_24310"
     CDS             complement(<1..838)
                     /locus_tag="PKO06_24310"
                     /inference="COORDINATES: protein motif:HMM:NF040131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV72854.1"
                     /translation="MNPILAENLRLISRHPRLVKTLEEFRSRPDAAEYLLEDARNGQK
                     CLQVREKGKAITYHSRYDPLQEARKQITGLRDDVSHVLVLGLGLGYQLEAILESLPSG
                     SQKPTILVVEPDPKVMLLALGCRSLARILADERIQWCVAQTPDDIGDHWSTYLDWASL
                     EHLCIIEHPPSLMRFHGYFERLKEKLRYLSNRSKGNLVTLMNAGFEFHTNNFVNCAVS
                     ASLPGVRRLFGRFAGLPAVVVAAGPSLEKNVALLAEIKDRFLIIAVDTAFRQLTVRGL
                     KPD"
     gene            complement(846..>1124)
                     /locus_tag="PKO06_24315"
     CDS             complement(846..>1124)
                     /locus_tag="PKO06_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72855.1"
                     /translation="KVREGMQHLVDVLDGMEWLSTILEHLDYSGKGLEENALALKKTE
                     LLKQIETQMTNLHASQEQQDWVGVADILEYEIQERFREGAELFRQVKG"
BASE COUNT          249 a          350 c          284 g          241 t
ORIGIN      
        1 tatccggttt caagcctcgc acggtgagct gacgaaaagc ggtatccacc gcgatgatca
       61 ggaagcgatc cttgatctca gccagcagcg cgacgttttt ctcaagagac gggccagcgg
      121 ccacgacaac ggccggcaga ccggcaaatc gcccgaacaa ccgcctgact cccggcagag
      181 aggcactgac agcacagttc acaaagttgt tggtatgaaa ctcgaaccca gcgttcatga
      241 gtgtcaccag gttccccttc gagcggttac tgagataacg cagcttttcc ttcaagcgtt
      301 cgaaatagcc atgaaaacgc atcagactgg gcggatgctc aatgatgcag aggtgctcca
      361 gtgacgccca gtccaggtat gtggaccagt ggtcgccgat gtcgtcgggg gtctgcgcca
      421 cacaccattg gatgcgctcg tctgccagaa tccgcgccaa agaacggcaa cccagggcca
      481 gcagcatgac cttcggatca ggctcaacga ccagaatcgt aggcttttgc gatccgctcg
      541 gcaggctctc cagaatcgct tctaactggt acccgagccc cagacccaaa acaagaacat
      601 gagacacgtc gtcgcgcagt ccagtaatct gcttgcgagc ttcctgaagc ggatcatagc
      661 ggctgtggta ggtgatcgcc tttcccttct cacgcacctg caggcacttc tgcccattgc
      721 gggcgtcttc cagcagatac tcggcggcat ccggtcgact tcggaattct tccaaagtct
      781 tgaccagccg tggatgacgg ctgatcagac gcagattttc cgccaggatc ggattcatgg
      841 atggattacc ccttcacctg tcgaaacagt tccgcccctt cgcgaaatcg ttcctgaatc
      901 tcatattcca ggatgtcagc cacacccacc cagtcctgct gttcctgcga ggcatgcaga
      961 ttggtcatct gcgtctcgat ctgtttgagc agttccgtct tcttcagggc caacgcattc
     1021 tcttcaagcc cctttcccga gtagtcgaga tgctcgagaa tcgttgacag ccattccata
     1081 ccgtccagca catccacgag atgctgcatc ccttcccgca cctt
//
LOCUS       DAORHD010001665         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191049_length_1119_cov_3.226504, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001665 DAORHD010000000
VERSION     DAORHD010001665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191049_length_1119_cov_3.226504"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1119
                     /locus_tag="PKO06_24320"
     CDS             39..>1119
                     /locus_tag="PKO06_24320"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV72856.1"
                     /translation="MTQTDTLPLMDPVSPTPSTLPVNAGSRGWKVFYAGLMIFAAFSG
                     FSISGMQIGLGIAFLGWCFCRWGEEVECKPVPFAWPYMALFAVSVLSLTNAVDLWRGF
                     TELKKYLIIIVFLMPFSARFSETQWKRLLALLVFSGALAGGIGLAGSIKNMLAQAQDF
                     RARGFYSMSITFGECQAMFLLLTLSWIKANLTYAKSNRWLWPAAILQFIGLLSSYSRG
                     AFIGFGVGLVLLIGPNWKRLVAIFAVLGLVIIAAAGIFRPNLDVYQVESQKPQSIGGN
                     LRWSIWKNGLSFWQQFPLLGVGLNNIKPHYKAFLKDHPDWPDKEAYGHLHNNFLQFLV
                     MTGILGLLTFVWLVLAIGKYAYYAWG"
BASE COUNT          204 a          286 c          313 g          316 t
ORIGIN      
        1 gttgcaattt ccctctgacg atgtgataat cgatccccat gacacagacg gacacgttgc
       61 cattgatgga tccggtgtct cctacaccct ctacacttcc agttaatgct ggttcaagag
      121 gctggaaggt cttttatgcc gggttgatga tcttcgccgc gttcagcgga ttctcgatct
      181 cgggcatgca gatcggcctg ggaattgcct ttctcggctg gtgtttctgt cgctggggcg
      241 aagaggtcga gtgcaagccg gttccctttg cctggccgta catggctttg ttcgccgtgt
      301 ccgtattgtc gctgacgaat gcggtcgatc tctggcgtgg cttcaccgaa ctgaaaaagt
      361 atctcatcat catcgtcttt ctcatgccgt tctcggcccg cttttctgaa actcaatgga
      421 agcgtctgct ggcactgctg gttttttcgg gtgcgctggc cggcggcatc ggtctcgcgg
      481 gctccatcaa gaacatgctg gcgcaggcac aggattttcg cgctcgcggt ttctattcga
      541 tgagtatcac gtttggtgag tgtcaggcga tgtttttgct cctgacgttg tcgtggatca
      601 aagccaattt gacgtatgcc aagtcgaatc gatggctgtg gccggcggca atcctgcagt
      661 tcatcggctt gctgtcgtcg tattctcgtg gagcttttat tggtttcggt gtcggactcg
      721 ttctgttgat cggcccgaac tggaagagac tggttgcgat ctttgcggtg cttgggcttg
      781 tcatcattgc cgccgcggga atctttcgcc ccaatctcga tgtgtaccag gtggaatctc
      841 agaaaccgca gagtattggc ggaaatctac gctggtcgat ttggaaaaac ggtctgtcat
      901 tctggcagca gtttccgctg ctgggagtgg gactcaacaa tatcaagccg cactacaaag
      961 cattcctcaa agaccatccg gattggcctg acaaagaagc ctacggtcat ttacacaaca
     1021 acttcctgca gttcctggtg atgaccggga tccttgggct tctcacgttt gtgtggctgg
     1081 tactcgccat tggaaagtat gcctattacg cctggggca
//
LOCUS       DAORHD010001666         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191109_length_1119_cov_2.574248, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001666 DAORHD010000000
VERSION     DAORHD010001666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191109_length_1119_cov_2.574248"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..418
                     /locus_tag="PKO06_24325"
     CDS             116..418
                     /locus_tag="PKO06_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72857.1"
                     /translation="MLKEMDVEQIIEEAKKFSRDTRFTDALAVLRVGMEKAPDNPQLM
                     MTAANIYTDLFLSKNDREAGKMARDLFTKIASLVPPDSKEGKLAQSMLQELSTRMQ"
     gene            complement(704..>1119)
                     /locus_tag="PKO06_24330"
     CDS             complement(704..>1119)
                     /locus_tag="PKO06_24330"
                     /inference="COORDINATES: protein motif:HMM:NF025179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HNV72858.1"
                     /translation="PELVRVEAQKVSEEAKQKTLNEAIAKVEKKLDSLPTLGLQYARL
                     MRDLKVREKVFATLTEQFEMAKIAEAEEGSSFEIIDRPRIPERKSKPSRALICILSAV
                     TAVMLSVFLAFFLEFLEKRRREEEKRQHAAVPQPS"
BASE COUNT          245 a          340 c          287 g          247 t
ORIGIN      
        1 gtgaagccgc caaacaggcc gccgcaaaag ccgccgaaaa agccgctctc gcccgctctc
       61 ggccttcctc gagaaacccg agaccgcctc gttcacccca cgcccgaagg atgtggtgct
      121 gaaggaaatg gatgtcgagc agatcatcga agaagccaag aagttttcgc gcgacacccg
      181 tttcaccgac gctctcgccg ttctgcgtgt cggcatggaa aaagcccccg acaacccgca
      241 actgatgatg accgccgcca acatttacac cgatcttttt ctcagcaaaa atgatcgcga
      301 agccggcaaa atggcccgcg atctctttac caaaatcgcc tcacttgtac cccccgactc
      361 caaagaaggc aaacttgccc agagcatgct gcaagaactc tccacccgca tgcagtagac
      421 tcgcgaacgc ttcctctccg ctttcgcgat tcactcccaa tcaggctcac atctggccca
      481 tcactgttgc gttaagttcg tcatcaccgg cgctgtcgtt tgtggcgcgc attacgcgac
      541 tattactcgc ctcccgtcgt tgtgagtggc ggtgacttcg caaacgtagg ttctgagtct
      601 ttcgtgtttc gtgatccgga gtttcctgtg agatggtcgt aacaggacaa gccccgaaac
      661 acacgtttcg gggcacagag acacacaaga ggacggtgca gcctcagctc ggctgtggaa
      721 ccgccgcatg ttggcgtttt tcttcttcgc ggcggcgttt ttcgaggaac tcgagaaaga
      781 acgccaggaa aacggaaagc atgaccgcgg ttacggcgct caggatacag ataagagcgc
      841 gactcggctt ggactttcgt tcagggatgc gaggacgatc gatgatctcg aacgagctgc
      901 cttcttcggc ttcggcgatc ttggccatct cgaactgttc ggtcagagtg gcgaacactt
      961 tttcgcgaac cttgagatct cgcatcagac gggcgtattg aagccccagg gtcggcaatg
     1021 aatcgagctt cttctcgacc ttggcaatcg cttcattcag cgttttctgt ttcgcttctt
     1081 ccgagacctt ctgcgcttcg acgcgtacca actcaggca
//
LOCUS       DAORHD010001667         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191357_length_1118_cov_3.218250, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001667 DAORHD010000000
VERSION     DAORHD010001667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191357_length_1118_cov_3.218250"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1118)
                     /locus_tag="PKO06_24335"
     CDS             complement(<1..>1118)
                     /locus_tag="PKO06_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNV72859.1"
                     /translation="SKVWDGQKRRVQKNVELIAKDLLELYAKREAHEGFAFPPDCELQ
                     EDLERRFPFEETPDQNKAIEDTKLDMQKSTPMDRLVCGDVGFGKTEVALRAAFKAVCA
                     GKQVAILAPTTLLAFQHEQTFKNRLADFPVRVGMVSRLVKPRDQKDLLEKAKDGRLDI
                     LIGTHRLLSADIGFKDLGLLIVDEEQRFGVKSKERLKQLRTTVDVLTLTATPIPRTMQ
                     MALSGIRQISIIDTPPEDRRPIQTYVAPFEAAWAKRALLDELRRGGQVYYVFNRVEKI
                     DQKAAFLRELVPEARIVIAHGQMDEELVEKTMLSFIRKEFDVLLATTIIESGLDIPNV
                     NTLIVEEADRLGLSQMYQLRGRVGRSYRQAYAYFFYSK"
BASE COUNT          220 a          332 c          306 g          260 t
ORIGIN      
        1 cttggagtag aaaaagtagg cgtatgcctg tctgtatgag cgcccaaccc gcccgcgcaa
       61 ctggtacatt tgagacagtc ccagtcgatc cgcttcctcg acgatcagtg tattgacatt
      121 cggaatgtcc aagcccgact cgatgatcgt cgtggccaga agcacgtcaa actccttgcg
      181 aatgaaactc agcatggttt tttccaccag ctcttcgtcc atctgcccgt gagcgatgac
      241 gatgcgtgct tcgggcacga gttcgcgcag aaacgcggct ttctgatcga tcttctcgac
      301 gcggttgaac acgtagtaca cctgcccccc gcggcgtaac tcatcgagca gcgcccgctt
      361 ggcccaggcc gcctcgaagg gggccacata cgtctggatc ggtcgccgat cttcgggagg
      421 ggtgtcgatg atgctgatct gtcgtatccc tgacagagcc atctgcatgg tgcgcggaat
      481 cggagttgcc gtcaatgtca gaacatcgac ggtcgtacgc agctgcttga ggcgttcctt
      541 gctcttcacg ccgaagcgct gttcttcatc gacgatcagc agaccgagat ccttgaaccc
      601 gatgtcggct gacaagagtc gatgcgtacc aatcagaatg tccagtctgc cgtctttcgc
      661 tttctcgagc agatctttct gatcacgggg tttgaccagt cgcgagacca tgccgacacg
      721 cacgggaaag tcggccagac gattcttgaa tgtctgctca tgctgaaagg ccagaagcgt
      781 ggtcggcgcc agtatcgcca cctgcttccc ggcacagacg gctttgaacg cggcgcgtag
      841 cgcaacttcg gttttgccga agccgacgtc gccgcagacg aggcgatcca tgggagtgct
      901 cttctgcatg tccagtttcg tatcttcgat tgccttgttc tgatcaggcg tttcttcaaa
      961 gggaaaccgc cgctccaggt cttcctgcaa ctcgcagtca ggcggaaaag caaagccctc
     1021 atgggcctcc cgcttggcat aaagttcgag cagatctttc gcgatcagtt cgacgttctt
     1081 ctgtacacgt cgcttctggc cgtcccagac cttgctgt
//
LOCUS       DAORHD010001668         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191625_length_1117_cov_3.955744, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001668 DAORHD010000000
VERSION     DAORHD010001668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191625_length_1117_cov_3.955744"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="PKO06_24340"
     CDS             complement(<1..1108)
                     /locus_tag="PKO06_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72860.1"
                     /translation="MADLRESHRRRKGAVLIERCRSLLGDRAPERRRMALVKLVELRQ
                     AGFTESQALLQAHRAVESSDELVLLINQALGETTESAVTTKQQELLTQLVTGDARAWR
                     EFETFAWSEKHDILRRGLALGDARADSFLKQVLLCVDPAVRSAAIDLAAGLVPTVLQP
                     FLTGLLQGEDSEIQGAALRALAVVDRARALPVFETFLFSAHAVRRAVAFDCLDSFDFS
                     TVRPLFVRAVCREQNDENRTCIETILMQHLEESLFHEVFACWRSDSVPVPALEPILKR
                     WAESLVQAGATRATDALSLWRTAAHLAEERRQRESSGAEYSVERIQSLRSSSKPEAEL
                     VVSVPLSAELVDLARPGATARFLTLWRMNAGNWPA"
BASE COUNT          252 a          358 c          306 g          201 t
ORIGIN      
        1 cggccggcca attaccagca ttcatgcgcc agagcgtcag gaaacgcgcc gtcgctcccg
       61 gacgcgccag gtcgaccaac tccgccgaca atggcaccga gaccaccagc tccgcttcag
      121 gcttcgatga cgaacgcagg ctctgaatac gttccaccga gtattccgcc ccggaacttt
      181 cacgctgtct gcgttcttcg gccagatggg cggcggtgcg ccacagcgag agagcgtcag
      241 tcgcccgcgt cgctccagcc tgcaccagac tctcggccca gcgcttcaaa atcggctcca
      301 atgccggaac gggaacggaa tccgaccgcc agcaggcaaa cacctcgtga aacagagatt
      361 cttcgagatg ctgcatcaga atggtttcga tgcatgtccg gttttcatca ttctgttcac
      421 gacagacggc tcggacaaac aacggtcgaa cggttgaaaa atcgaagctg tcgagacagt
      481 cgaaagccac ggcgcgtcgt actgcgtgcg ccgaaaagag aaacgtttcg aaaaccggta
      541 acgcccgcgc tcgatcgacg actgccagag cgcgcaatgc ggcgccttgg atctcggaat
      601 cctcaccctg caaaagaccg gtcagaaagg gttgcagcac ggtcggaacc agcccggcag
      661 ccagatcgat ggcagcagag cgtacagcag gatcgacaca gagcagaacc tgcttcagaa
      721 agctgtcggc acgggcgtca cccagagcca acccacgtcg caggatgtcg tgtttctcgc
      781 tccaggcaaa ggtttcgaac tcacgccacg ccctggcgtc tccggtcacc agttgtgtca
      841 gcagttcctg ctgtttcgtg gtgactgccg attcggtggt ctctcccaac gcctggttga
      901 tcaacagtac taactcgtct gacgattcaa ccgctcgatg tgcctgcaag agagcctgag
      961 attcagtgaa accagcctga cgcaactcga ccagtttcac cagagccatg cggcgacgtt
     1021 ccggcgcacg gtccccgagc aatgatcgac atcgttcgat cagcacagct cctttgcggc
     1081 gacgatgaga ttcgcgcagg tctgccatca cctcttc
//
LOCUS       DAORHD010001669         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191929_length_1116_cov_4.132893, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001669 DAORHD010000000
VERSION     DAORHD010001669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191929_length_1116_cov_4.132893"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1116)
                     /locus_tag="PKO06_24345"
     CDS             complement(<1..>1116)
                     /locus_tag="PKO06_24345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72861.1"
                     /translation="VNARNVETLIREKEAHRLARFSLDHANDIVFWALADGRVMDVNQ
                     RAETALGHSREVLTRLTTTQLASLQGDLAWNLLWQKLQLKGSVVFEAALRRSDGALLP
                     TEFSVFGFQFEGIDYACVFARDISERLKIEEDRRRLMQRMQQSQKLEGLGTLAGGIAH
                     DFNNLLMVITGNAYLGQEEVEREDSAYKRFQAVIEAADRAADLCRQMLAYAGKAPARF
                     ESLSTNETIAGMVGLLRTLVGKRARLDIHLDARNPFIRGDRTQVQQVLLAIVTNSLEA
                     CSEMNGEIGVMTGIVELSRADFEAIDFEEPPTEGMFAFIEVSDNGCGMTDEQRHRIFD
                     PFFTTKFQGRGLGLAAVAGIVRAHRGGRQVKSRPGEGT"
BASE COUNT          233 a          358 c          294 g          231 t
ORIGIN      
        1 gttccctcgc cgggtctact cttgacctgc cgcccgcccc gatgcgcgcg cacgatgccg
       61 gccactgccg ccagcccgag accgcgccct tgaaatttcg ttgtgaagaa cggatcgaaa
      121 atgcgatgtc gttgctcatc ggtcatgccg caaccgttat ctgaaacctc gatgaaggca
      181 aacatgccct cggtcggcgg ttcttcgaaa tcgatggctt cgaagtctgc tcgactcagc
      241 tcgacgattc ccgtcatgac gccgatctca ccgttcatct cggaacacgc ctcaagcgag
      301 ttcgtgacaa tggccagcag tacctgttgc acctgcgttc gatcgccgcg aataaacggg
      361 ttgcgtgcat cgagatgaat atcgagcctg gcccgcttgc caacaagtgt tcgcagcaga
      421 ccaaccattc ccgcaatcgt ttcgttggtc gagagactct cgaagcgagc cggagccttt
      481 cccgcatagg caagcatctg tcgacacagg tccgccgcgc gatctgccgc ctcgatcact
      541 gcctgaaagc gcttgtaggc actgtcttcc cgctcgacct cctcctgtcc gagataggcg
      601 tttcccgtga tcaccatcag caggttattg aaatcatgcg caataccacc cgccaaagtt
      661 ccgagaccct cgagtttctg agactgctgc atgcgctgca taagtcgacg ccggtcttct
      721 tcgatcttca ggcgttctga gatatcgcgc gcaaacacgc aggcatagtc gatgccctcg
      781 aactgaaaac cgaacacgct gaattccgtg ggcaacaagg ctccgtctga tcgacgcagg
      841 gcggcttcga acacgacgct gcccttgagc tgaagcttct gccaaagcag gttccaggcc
      901 agatcgccct gcagagaggc caattgcgtg gtggtcaggc gggtcagcac ttcgcgagaa
      961 tgcccgagag ccgtctccgc tctctgattc acatccatga cccgaccgtc agccagcgcc
     1021 cagaacacga tatcgttcgc atgatcgagc gaaaagcgag cgagacggtg ggcttccttt
     1081 tcgcgaatca gagtctcgac gttgcgcgca ttcacc
//
LOCUS       DAORHD010001670         1116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_191961_length_1116_cov_3.232799, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001670 DAORHD010000000
VERSION     DAORHD010001670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_191961_length_1116_cov_3.232799"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PKO06_24350"
     CDS             complement(<1..211)
                     /locus_tag="PKO06_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72862.1"
                     /translation="MKRHLSSFSVAHQRWLALLSMCLLALCWLRPLTDSEQRLVKAPT
                     NNWQVLRSVAPATLTVTLATEPVPHL"
     gene            complement(348..>1116)
                     /locus_tag="PKO06_24355"
     CDS             complement(348..>1116)
                     /locus_tag="PKO06_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72863.1"
                     /translation="FFGQKASYGMIPFLTIGALIGCLTIIYVVAQRQQTFGRPGRVCS
                     FLASAAVFIQLGIMLSYYWGNLGHRYAYRFGIIYVPVIIILNLFLVNLLDTSKWRTRI
                     LVAVACLGILIYYRPVAGMNASAQGMLIYREFQQVRTFLEHRYPQQNILLISHRPSLF
                     TPFRWGAISTDFARLHADQIQQRWQKGSFRDIVIHQCIRHRDGLIVDPDTDQQIQLTS
                     TATASSSATSLYAKTELLSVTELSEDYQLRLLRVCPP"
BASE COUNT          288 a          271 c          330 g          227 t
ORIGIN      
        1 caagatgggg aacgggttct gtcgcgaggg tcacggtcag cgtcgcggga gcaacggagc
       61 gaagaacctg ccagttgttc gtgggcgctt tcaccaggcg ctgttccgaa tccgtcagag
      121 ggcgtaacca gcagagcgcc agcaggcaca tgctcagaag agccagccat cgttggtggg
      181 ccacggaaaa tgaggagagg tgacgtttca tttcaacctg ctgcccgaac ggtgtttcct
      241 cttgagaaaa tcgttaggcg cattgtacgc catctgaagc gccgaatgcc aggagatggg
      301 agagatgatc gtgcgcgaac gggacctcaa ccccttcact caccacatca tggtgggcag
      361 acgcgcagca gtcgcagctg atagtcttcg ctcaactcgg tgaccgacag caattcggtc
      421 ttcgcataga gcgaggttgc cgaggatgac gcagtggctg tcgaagtgag ttgtatctgt
      481 tgatcggtat ccgggtcaac gatcaaccca tctcgatgtc gaatgcactg atgaatgacg
      541 atgtcgcgaa aggagccttt ctgccagcgc tgttgaatct gatcggcgtg aagtctggca
      601 aaatccgttg atatggctcc ccagcggaac ggagtgaaca acgaaggacg gtgggagatg
      661 agcagaatat tctgctgggg ataccgatgc tcgagaaagg tgcgaacctg ctgaaactca
      721 cggtaaatca acatgccttg agcactggca ttcatgcctg ccaccggacg atagtagatg
      781 agtatgccca ggcaggcgac tgccaccaga atgcgcgtgc gccattttga tgtgtctaac
      841 aagttcacca gaaaaagatt gagaatgata atgactggta cgtaaatgat accgaatcgg
      901 tatgcgtagc ggtgtcccag gtttccccaa tagtagctga gcatgattcc tagctgaata
      961 aaaacagctg cagatgcaag gaacgaacaa acccttcctg gacggccgaa agtttgctgc
     1021 cgttgggcca caacatagat tatggtcaga catccgatga gagccccgat ggtcagaaag
     1081 gggatcatgc cgtaggacgc tttctggccg aaaaaa
//
LOCUS       DAORHD010001671         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_192257_length_1115_cov_3.145283, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001671 DAORHD010000000
VERSION     DAORHD010001671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192257_length_1115_cov_3.145283"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..894
                     /locus_tag="PKO06_24360"
     CDS             187..894
                     /locus_tag="PKO06_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72864.1"
                     /translation="MPFSPCLRDDPDLICQPADDPIRALLDLVCKWVFYLVVVLLFIN
                     FDTKHRMPYSLMPYIWKLSYFGLGALILKHTTDNHYAIDLNAKTISYHFHVLFFRRVL
                     HICKFKDVDRIMVDVIKRNPKDAFDLEYQIILLHYYGHAMQMRSFSELSQDKEDLTSR
                     AIELSQMTGIPVGDWGRGFFAHPTRKRMLDPIQQHLLRRSQPIHDLDLKILIHVVAGA
                     IIALAGFSAWLLVRFFP"
BASE COUNT          235 a          341 c          260 g          279 t
ORIGIN      
        1 ccgggcggaa ccgtgcgggc tagtcttggc gcgctttctt ctcacgaaga tatcgaatct
       61 ctggtacaag ctatgcaagt tcttcctatt tgacagaaac tcggacattc agcatgatgc
      121 aaacggtgat caaagagttc ttcgccttcc gtgacggggc gcattcatca agtgagggac
      181 catttcgtgc cattttctcc gtgtctgcgc gatgatcctg acctgatctg ccagccggcg
      241 gatgacccca ttcgcgctct tctcgacctc gtctgcaaat gggtcttcta tctcgtggtc
      301 gtgctgctgt tcatcaactt cgacaccaag catcgcatgc cctattccct catgccgtat
      361 atctggaaac tgtcatactt cggcctcggc gcgctgattc tcaaacacac cactgacaat
      421 cattatgcga tcgacctgaa cgcgaaaacc atctcgtacc attttcatgt tctgttcttt
      481 cgccgcgtcc tgcacatctg caagttcaag gatgtcgacc gaatcatggt cgatgtgatc
      541 aagcgaaacc cgaaagacgc atttgatctc gaatatcaga tcatcctcct ccactactac
      601 ggacatgcca tgcaaatgcg ctcgttttcc gagctgtcac aggacaagga agatctcacc
      661 tctcgcgcga tcgaactctc ccaaatgacg gggattcccg tcggtgattg ggggcgtggc
      721 ttcttcgctc acccgacccg caaacgcatg ctcgacccca ttcagcagca tcttctgagg
      781 cgatcccagc cgattcatga cctcgacctg aaaattctca ttcatgtcgt tgccggtgcc
      841 atcattgcgt tggccggctt cagtgcctgg cttctggtgc gctttttccc atagggcacg
      901 cggccagaaa acgtgccacg cccagcagac gagatggcgt cacgacatcg gcctcagcgc
      961 gtcgtcttcg ttcccgaact ggtaaaaccc ttcccgatca ttttgagatg atcacccttg
     1021 tcgatcgcca cggcgttccc gcagacacag aacacgcgtt cttcgtcgcg aaccagttca
     1081 tacattcggg tgatgcgatc tttgtgacag accag
//
LOCUS       DAORHD010001672         1115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_192318_length_1115_cov_2.648113, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001672 DAORHD010000000
VERSION     DAORHD010001672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192318_length_1115_cov_2.648113"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..>1115
                     /locus_tag="PKO06_24365"
     CDS             78..>1115
                     /locus_tag="PKO06_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72865.1"
                     /translation="MAIEIRETDGSRWFAVVELTAAWQKHILQPGDFKYWPDSPTRNR
                     GSSGDRMNSQNATVISFGLAQSHTSAVKPGSHTFFVDQVGTAADPFPAEVRKKTETAS
                     PLIETISPAYKVYELSDVAAVRAAPEQGIIPEHLDVALPSSATSPHVRLAGRGYTDRA
                     KWRFIPLLEAFDKNNHKLGVPFSLLITHTDEFKASVTANLGISGASIQDPSLTAAVVS
                     AVDRIQHGLFLFNAGSQQFAYWPGQEVILGCRVINASPLARTAQIRITVLQTDSAETV
                     FTRTVRLDTAPRAVAEFACDWQPPSSPARYIVRTELLNEGQVHDAIRHELTVLPELRP
                     ARNDFVTVRNGD"
BASE COUNT          227 a          388 c          298 g          202 t
ORIGIN      
        1 cgccgataac cgaattgttc acccccggtc attcgctgct gtatctgcgg gcgcggggcg
       61 gccccgaaac cccacaaatg gccatcgaga tccgcgagac cgacggctct cgctggtttg
      121 ccgtagtgga actgactgcg gcgtggcaga agcacatcct ccaacccggc gatttcaaat
      181 actggcctga ctcccccacc cgcaaccgcg gctcatccgg cgaccgcatg aactcacaga
      241 atgccacggt tatcagtttc gggctggcgc aatcgcacac ttcggcagtg aagcccggct
      301 cgcacacctt cttcgtggac caggtcggca ccgctgccga tcccttcccc gccgaggtcc
      361 gcaagaagac cgaaaccgcc tcgccgctga tcgagaccat cagcccggca tacaaagtct
      421 acgagctgag cgacgtggcc gccgtcagag cggcacccga acagggaatc atcccggaac
      481 atctcgacgt cgctctgccg agttcggcga cgtcgcccca tgtgagactt gccggccgag
      541 gctatacgga cagggccaag tggcgattca tccccctgct cgaggcgttc gacaaaaaca
      601 accacaagct cggagtacct ttctcgcttc tcatcacaca cacagatgaa ttcaaggcct
      661 ccgtcacggc gaatctcggc atcagcgggg catcgatcca ggacccgagt ctgacggcgg
      721 cagttgtctc tgctgtcgat cgaatccaac acggtctctt cctcttcaac gcaggctccc
      781 agcagtttgc atactggccg ggtcaggagg ttatcctcgg ctgccgcgtg atcaacgcat
      841 cgcctctcgc cagaaccgcc cagatcagaa tcaccgtttt gcagaccgac tcggccgaaa
      901 cggtcttcac gcgaacggtc cgcctggata ccgcgccccg tgctgttgct gaattcgcat
      961 gtgactggca gccaccatcc tcgccggccc gctacattgt ccgcaccgaa ttgctcaacg
     1021 aagggcaggt ccatgacgca atcaggcacg agttgactgt cctgccggaa ctccggccgg
     1081 ctcgcaatga cttcgtcacc gttcgcaatg gcgac
//
LOCUS       DAORHD010001673         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_192529_length_1114_cov_3.898017, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001673 DAORHD010000000
VERSION     DAORHD010001673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192529_length_1114_cov_3.898017"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..>1114
                     /gene="murD"
                     /locus_tag="PKO06_24370"
     CDS             312..>1114
                     /gene="murD"
                     /locus_tag="PKO06_24370"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNV72866.1"
                     /translation="MEIRGRQVSVIGAGKSGLVASKVLAELGARVLLSDSAPRDRLET
                     VLHQAALPESIEIESGGHTERVLSADFIVLSPGVPGTIPILQKAQQRGIPILSEIEIA
                     YRLSRGRRLVITGSNGKTTTTTLLGKFCETGFPRVFVGGNIGTPMMEFALSTRENDIQ
                     VLEVSSFQLETISEFTPDIAMITNFYENHLDRYVSYEAYKAAKERIALNMRGDQWLIL
                     NADQRESREVASRAQCSIAWFGWQLEDRRPGMTFEGTTLIFVSADGKRTP"
BASE COUNT          246 a          281 c          325 g          262 t
ORIGIN      
        1 aaggtatgga gaacgtgaat cagccttctg tcatccccga gagcacaaac gctcctcaag
       61 ggatgacaga aggctgattc acgttctcca ttccttggcc gaaacgacaa cgaaacattg
      121 attgttgtga ggtcagtgcc ctgttgtgtc cgaaaaggtg aagccgtcta tctgcatcat
      181 ctgctggttt ggccatgtcg gttgtgtcgc gatgagtcgg aaaaaacctg aacaacgtgc
      241 attgttcgtg cccctttcct gtgctagtat gatcccttcc ctgaatctgt tgggtcgaag
      301 gaagtctcgt catggaaata cgtgggcgtc aggtcagcgt catcggtgct ggaaaaagtg
      361 gtttggtagc atcaaaagtg cttgccgagc ttggggcacg ggttctgctt tcggattccg
      421 cgcctcgaga ccgcctcgag accgttctgc accaggcggc attgcctgaa tctatcgaga
      481 ttgagagcgg agggcacacg gaaagagtgc tctcggcgga tttcatcgtt ctgagcccgg
      541 gagtgccagg caccattccg atcctgcaga aggcgcaaca gcgcggcatt ccgattctct
      601 ccgagattga gattgcctat cggctttcgc gcgggaggcg gttggtgatc accggcagca
      661 atggaaagac cacgacgacg accttgctgg gaaagttctg cgaaaccggc tttccacgtg
      721 tgtttgtcgg agggaacatt ggcaccccga tgatggaatt cgctctcagc acgcgcgaaa
      781 acgacattca ggtgctcgag gtcagcagtt ttcaattgga gacgatctct gagtttacgc
      841 ctgacattgc gatgatcacg aatttctacg agaatcatct cgatcggtat gtttcatatg
      901 aagcatacaa agcggcaaaa gagcgcatcg ctctgaatat gcgcggtgat cagtggctga
      961 ttctcaatgc cgaccagcgc gaatcgcgcg aggttgcctc tcgggcgcag tgcagtattg
     1021 cctggtttgg ctggcaactc gaggatcgcc gaccgggaat gactttcgag ggcacgacgc
     1081 tgatctttgt cagtgctgat ggcaagcgca cccc
//
LOCUS       DAORHD010001674         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_192542_length_1114_cov_3.552408, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001674 DAORHD010000000
VERSION     DAORHD010001674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192542_length_1114_cov_3.552408"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..>1114
                     /locus_tag="PKO06_24375"
     CDS             175..>1114
                     /locus_tag="PKO06_24375"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955628.1"
                     /note="Catalyzes a two-step reaction, first charging a
                     glycine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HNV72867.1"
                     /translation="MVDLDKIISLCKRRGFLFQGSEQYGGLQGTYDYGPLGVELKNNV
                     KRAWWRRMVYERDDMEGLDCAILMNRLVLKYSGHEETFSDPMVDCRKCKRRFRADQVD
                     ADEKCAEGGKHDLTEPRPFNLMFKTIVGPVQDDGNIAYLRPETAQGIFVNFKNVLDST
                     NRKLPFGIAQIGKAYRNEITPKNFIFRVREFEQMEIEFFVKPGSDDEWHRRWVEDRYN
                     WYISLGIKPENLRKYEQKPQELAHYAKATTDLLYRFFPDRPDEAKQFDEVEGIANRTD
                     FDLTCHSKGGKLVLKDGRVIPNEHAVAKLTYFDHETN"
BASE COUNT          278 a          296 c          323 g          217 t
ORIGIN      
        1 gatgtcgggg gcgacgttcg ggctggatgc gcagtagatg cagacggcac ggggatcgag
       61 aggaaattca cgggtttggt gcatgtcgca acagtaccat gttgaaagcg gcctgacaag
      121 catctataat gagccacact ttacgcctta cggcagatca ggagaaacag cgtcatggtc
      181 gatctcgata aaatcatctc cctgtgcaaa cgccggggat tcctgttcca gggaagcgaa
      241 caatacggcg gcctgcaggg aacgtatgac tacggcccgc tcggggtcga actgaagaac
      301 aacgtcaaac gcgcgtggtg gcgtcgcatg gtctatgagc gcgatgacat ggaaggtctc
      361 gactgcgcca ttctcatgaa tcgtctggtg cttaaatact cgggtcacga agaaaccttc
      421 tcggacccca tggttgattg ccgcaaatgc aaacgccggt tccgcgccga ccaggtggat
      481 gccgacgaga agtgcgccga aggcggaaaa catgatctga ccgagccgcg ccccttcaat
      541 ctgatgttca agaccatcgt cgggccggtg caggatgacg ggaacatcgc ttatctgcgc
      601 cccgaaacgg cgcagggcat cttcgtcaac ttcaagaacg tgctcgattc gacgaatcgc
      661 aagctgccct tcggcatcgc gcagatcggc aaagcgtatc gcaacgaaat cacgccgaag
      721 aacttcatct tccgcgtgcg cgagttcgaa cagatggaga tcgagttctt tgtgaaaccc
      781 ggttccgacg acgaatggca tcgccgctgg gttgaagatc gctacaactg gtacatctca
      841 ctgggtatca aacccgagaa cctgcgcaag tacgagcaga agccgcagga gctggcgcat
      901 tacgccaagg cgaccaccga tctgttgtat cgctttttcc ccgatcggcc tgatgaagcc
      961 aagcagtttg atgaagtcga gggaattgcg aaccgcacgg atttcgatct gacctgccat
     1021 agcaagggtg gcaaactggt actcaaagat ggacgggtga ttccaaacga gcatgccgtg
     1081 gcgaagctga cgtatttcga tcatgaaacc aatc
//
LOCUS       DAORHD010001675         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_192547_length_1114_cov_3.416431, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001675 DAORHD010000000
VERSION     DAORHD010001675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_192547_length_1114_cov_3.416431"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1114
                     /locus_tag="PKO06_24380"
     CDS             <1..>1114
                     /locus_tag="PKO06_24380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV72868.1"
                     /translation="FVIDLFLPSLMMMVQFALVRFMQMYWSLQEAYGSLQERTVDLEQ
                     TNRTLDQQVRDLSQLNLAGNRFSTTLEIDSLCQEVMSTFRSLWESPVCVIGVFDQETE
                     QIQLLRQTGGASDEEVRLTLYDPGVVRSLQRAVESRQVVVEMNANRWFNCFVPLPLGA
                     RVWGALLLNSPRPREILQDREDFWSTLSGLAATALENSRLYNLATVDTLTKMYVRRYL
                     QVTIDQEFKRARRFNHAISVLMIDIDQFKSFNDTFGHQQGDVVLREVSQVVRRSLRDI
                     DIPARYGGEEFCVVLPETNADGALIVAERIRRNVEQLCVPRLNSPGEPLRVTVSIGGT
                     AFPELNAENADQMIGVADAALYDAKRQGRNRTIISGG"
BASE COUNT          236 a          284 c          337 g          257 t
ORIGIN      
        1 tttcgtcatc gatctgtttc tgccaagcct gatgatgatg gtgcaattcg ctctggtgcg
       61 tttcatgcag atgtactgga gcctgcagga agcctatgga tcgctgcagg aaagaaccgt
      121 ggatctcgaa cagaccaatc gaactctcga tcagcaggtt cgagatttgt cccagttgaa
      181 tcttgcggga aatcgctttt caacaacact tgaaattgat tcgttgtgcc aggaagtgat
      241 gagcacgttt cgatcgctgt gggaatcgcc ggtgtgcgtg atcggggtgt ttgaccagga
      301 gacagaacag attcagctgc tgcgacaaac cggtggcgct tcggacgaag aggtgcgcct
      361 gacactctat gatccgggag tggtgcgcag tctgcagaga gccgtggaat cgcggcaggt
      421 cgttgttgag atgaatgcca accgctggtt caactgcttt gtgccgttgc ccttgggggc
      481 gcgggtgtgg ggagccttgc tgttgaacag cccccgtcca cgcgagattc tgcaggaccg
      541 cgaggatttc tggtcgacac tgtcggggct tgcagccacc gcgcttgaga actctcggtt
      601 gtacaacctc gccaccgtcg atacgttgac caagatgtat gtgcgccgct atctgcaggt
      661 aacgatcgat caggagttca agcgggcacg ccgcttcaat catgccatct cggtactgat
      721 gatcgatatt gatcagttca aatcgttcaa cgacacgttt gggcaccagc agggagatgt
      781 cgttctgcgt gaagtctcac aagtggtgcg ccgctctctg cgcgacatcg acattcccgc
      841 acgatacggt ggcgaggaat tctgcgtcgt gttgccggaa accaatgccg atggggcatt
      901 gattgtcgcg gagcggatca gacgcaatgt cgaacagttg tgcgtccctc gtctcaactc
      961 tcccggagaa cccctgcgcg tgacggtcag tatcggcggc acagctttcc cggaactgaa
     1021 tgccgaaaat gctgatcaaa tgattggtgt cgctgacgct gccctgtacg atgcgaaaag
     1081 gcaggggcga aatcgtacga tcatcagcgg cgga
//
LOCUS       DAORHD010001676         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_193173_length_1112_cov_3.035951, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001676 DAORHD010000000
VERSION     DAORHD010001676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193173_length_1112_cov_3.035951"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..>1112)
                     /locus_tag="PKO06_24385"
     CDS             complement(29..>1112)
                     /locus_tag="PKO06_24385"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV72869.1"
                     /translation="RCRLTGALALYTIPVIKGSRRPYMKKHAMTLLICASVLVGPAFG
                     AEKSGAVKGSYVNIRKTNAFSGEVVGKKLRGEKYTILFEESGWRKVQFEDGISGWIFM
                     GSKGGEGGEGNEETDSVDGTVNAKGSDSGIATLTSLASLEDEYRSPSQPTEVKEPPKE
                     KKEDGKGKKPTKSKKPKGTSFGLATAPAKILVSSKTAEEWYNEGIEKFEQKKYPEALE
                     CNQEALKLAPGNAEILNNLANCLFKMGRVDEALTQWKEALSIAPRSAKICNNMGIAYY
                     QKEDNAKAVDYYKKAVLFEPQFPDPYYNLGSVYGFMGKYKEALENYKKYLEFSPDPTM
                     RSLTEERIEYCQKQLGAKKAPASTKK"
BASE COUNT          207 a          307 c          274 g          324 t
ORIGIN      
        1 cgtgtctttt ccggcgcatc ggcagcgcct attttttggt cgatgccggg gctttcttgg
       61 ctccaagctg tttctggcag tattcgatgc gttcttcggt cagcgaccgc attgtcgggt
      121 caggtgaaaa ctcgagatac ttcttgtaat tctcgagagc ttctttgtac tttcccatga
      181 agccgtaaac agaacccaga ttgtaatatg ggtcaggaaa ctggggctca aacagcacgg
      241 ccttcttgta ataatcgacg gccttggcat tgtcttcctt ctgataataa gcgatgccca
      301 tattattgca gatcttggct gagcgcggcg caatggaaag agcttctttc cattgggtca
      361 acgcttcatc aacacggccc attttgaaaa gacaattcgc gaggttattg agaatctccg
      421 cgtttccagg agccagcttc aacgcttcct gattgcattc cagcgcttca ggatactttt
      481 tctgttcaaa cttttcgata ccctcgttgt accattcctc ggcggtcttg ctcgagacca
      541 gaatcttcgc gggggcagtt gccaagccga acgaggtgcc cttgggcttc ttcgactttg
      601 tcggtttctt acctttgccg tcttctttct tctctttcgg tggctctttt acttcggtgg
      661 gttgagacgg gcttcgatac tcgtcttcca gcgatgccag cgaggtcaga gtggcaatgc
      721 cgctgtcgct gcctttggca ttgacggttc cgtcgacgct gtcggtctct tcgtttcctt
      781 cgccgccctc gccgcccttg ctgcccatga agatccagcc gctgatgcca tcttcgaact
      841 gcactttgcg ccaaccggat tcttcgaaca gaatcgtgta tttttcgccg cgaagttttt
      901 tcccgacaac ttctccggaa aaagcattgg ttttgcggat gttgacgtaa ctccccttca
      961 cggcaccgga cttttctgcg ccaaatgccg gcccgaccag cacagaggcg caaatcagca
     1021 gtgtcatcgc gtgttttttc atatacggtc tccttgagcc cttgatcacg gggatggtat
     1081 acaacgcgag agcgcctgtc aagcgacatc tg
//
LOCUS       DAORHD010001677         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_193246_length_1112_cov_2.412488, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001677 DAORHD010000000
VERSION     DAORHD010001677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193246_length_1112_cov_2.412488"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKO06_24390"
     CDS             complement(<1..319)
                     /locus_tag="PKO06_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HNV72870.1"
                     /translation="MNFLFLSPHFPPNFTCFSEQLNLAGFNVLGMGDAPWHELPDRLK
                     WSLKEYCQIQNMEDYDQVYRATAYLISRFGRIDRVGSQNEHWLRLEARLRDDFSIPGM
                     RPLE"
     gene            477..>1112
                     /locus_tag="PKO06_24395"
     CDS             477..>1112
                     /locus_tag="PKO06_24395"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HNV72871.1"
                     /translation="MTRVFASILLVACALCLCFPGSVAAGSLEETLSTSALTEAQLKG
                     KTPAELDILRNHIYARHGYIFKRGDLKQYFSEFGWYKPEISDSAAIEKKLNATEKQNI
                     ALLKKLESQKPSDSSAGKTGSSPATCGEFIDNESNIRSEPAVKAKLLGVSKTGERFDV
                     LDSKDPWYYIKTTGGITGWTHKKNIKLSLCRPASTEGVETPNTPDEAGPPND"
BASE COUNT          279 a          332 c          288 g          213 t
ORIGIN      
        1 cttcgagagg gcgcataccg ggaatgctga agtcgtcgcg cagtcgcgct tcgagtctca
       61 accaatgctc gttctgagag ccaacccgat cgatgcggcc aaaacggctg atcaggtagg
      121 cggtcgctcg atacacctga tcgtaatctt ccatgttctg aatctgacaa tactctttca
      181 gcgaccactt cagacggtca ggcagttcgt gccacggcgc gtctcccatt ccaagaacgt
      241 tgaacccggc cagattgagc tgttcactga agcaggtgaa gttcggcggg aaatggggtg
      301 acaaaaagag aaaattcatg gtttggctcc tcgcgagtgt cgggttccga aaccggccga
      361 cggaatcgga ccttccgatc ttaccacaaa acccacacaa ccggcagcgg aatgcgatac
      421 aataccgacc agcattcgct gacttcatca cgcactctgc gacaacggag gatttcatga
      481 cacgcgtctt tgcctcgatt ctgctggttg cctgcgccct gtgcctctgc ttccctggtt
      541 cggtcgcggc tgggtcactc gaagaaaccc tgagcacctc ggctctcacc gaagctcaac
      601 tcaaggggaa gacccccgcc gagctcgaca ttctgcgcaa ccacatctat gcccgccatg
      661 ggtatatctt caagcgcggc gacctgaagc agtatttctc tgagttcggc tggtacaaac
      721 ccgaaatcag cgattccgcg gcaatcgaga aaaagctgaa cgccacggag aaacagaata
      781 tcgctctctt gaagaaactc gagagccaga agccgtctga cagcagtgcc ggaaaaaccg
      841 gttcgtcacc ggcaacgtgc ggcgagttca tcgataacga aagcaacatt cgttcagagc
      901 cggctgtgaa agccaagctg cttggggttt ccaaaaccgg cgaacgattc gacgttctcg
      961 acagcaaaga tccctggtat tacatcaaga ccaccggtgg catcaccggc tggacccaca
     1021 agaagaacat caagttgagt ctctgccgac ccgcttccac cgaaggggtt gaaacaccga
     1081 acacccccga tgaagccggg ccgccaaacg ac
//
LOCUS       DAORHD010001678         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_193816_length_1110_cov_2.582938, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001678 DAORHD010000000
VERSION     DAORHD010001678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193816_length_1110_cov_2.582938"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1110
                     /locus_tag="PKO06_24400"
     CDS             <1..>1110
                     /locus_tag="PKO06_24400"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV72872.1"
                     /translation="NFRSTPAVIQACNRLFADDFFSGEGIRYDNPVLPGRPDLRLTDH
                     GGQTQPAIELLPLPATATTVAQRQTILARAISNSIQRLLSPSGALYLFENGKTRTRIQ
                     GNSIFILTRKAAESRLIARTLREDGIPCAFFKEEGLFQSPEARAVAELLDAILHPEDQ
                     TRINRALLTPFFGCRPEALLAFRESAHGREAFSRLASWHQLALNGDVAVLLDRIRTES
                     GVVRRLLLMEDSYRTLANILHLFEILTEEAESGRAAVADLWRDLVHWSTLSGETIGGL
                     NNLQRLETDLEAVQILTMHKSKGLEAGIVYLFGGFTDFRDSLPLVIRAGASRAVSGPL
                     HPRDALVLDAVRRSENERLLYVALTRAKGRLCLPVE"
BASE COUNT          208 a          352 c          311 g          239 t
ORIGIN      
        1 aacttccgct caaccccggc ggtcattcag gcctgcaacc gtcttttcgc cgatgacttc
       61 ttttcgggag agggaatccg gtatgacaac cccgtccttc ccggtcgccc tgatctgcgg
      121 ttgaccgatc atggcggtca gacgcagccc gccatcgaac tcctgccact gccggcaacc
      181 gcgacaacgg tcgcccagcg ccagaccatc ctggcgcgtg ccatctcgaa cagcattcag
      241 cgcttgctgt caccgagcgg agctctctat ctgttcgaaa acggcaaaac ccggacacgg
      301 atacagggca acagcatttt tatcctgacc cgcaaagcgg cggaaagccg gttgatcgcc
      361 cgcaccctgc gtgaggacgg cattccctgc gcgtttttca aagaagaagg gctgtttcaa
      421 agcccggaag cgcgtgccgt ggcggaactg ctggatgcca ttctgcatcc cgaggatcaa
      481 acccgtatca atcgtgcgtt gttaacgcca ttctttggtt gccgtccaga agctctgctg
      541 gcgttccggg aatcggctca cggccgcgaa gcgttctccc gtctggcatc ctggcatcag
      601 ctggctctga acggagatgt cgccgttctg ctcgatcgca ttcgcaccga gtcgggagtc
      661 gttcgccgac ttctgctcat ggaagactca taccgcaccc tggccaatat cctgcatttg
      721 ttcgagattc tgaccgaaga ggcggagtcg ggtcgagccg ccgttgcaga cctctggcgt
      781 gatctggttc attggtcaac gctttcgggt gagaccatcg gtggtctcaa taacctgcag
      841 cgcctcgaga ccgatctcga agcagtgcag attctcacca tgcataagag caaggggctc
      901 gaggcgggca tcgtgtatct attcggagga ttcaccgact ttcgcgattc tctgcccctg
      961 gtgattcgcg ctggcgcatc acgagccgtc tccggcccat tgcatccccg tgacgcgctc
     1021 gttctggatg ccgtgcgccg cagcgaaaac gagcggcttc tctacgtagc cttgacccgt
     1081 gccaaaggcc gattgtgcct gccagtcgag
//
LOCUS       DAORHD010001679         1110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_193829_length_1110_cov_2.450237, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001679 DAORHD010000000
VERSION     DAORHD010001679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_193829_length_1110_cov_2.450237"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PKO06_24405"
     CDS             <1..211
                     /locus_tag="PKO06_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72873.1"
                     /translation="AEAKKKMNKLDADASGRIILLRTLLSPYEEDVTRLDGEKIKNEA
                     TELSRIIDEMRVQKDRISRMERDLE"
     gene            211..621
                     /locus_tag="PKO06_24410"
     CDS             211..621
                     /locus_tag="PKO06_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72874.1"
                     /translation="MAKKQAYFAEAERLYVVEQMTLTEIAARLKLCERTVRTWKDEGS
                     WETRRAQFMASKESFHTELYDFARKLMRTIRDDIDAKEKVDAGRLYTFARLLPRLTKI
                     KEYEDVVGGEKPKEQKPLDQKELVDAVQKALLGE"
     gene            624..>1110
                     /locus_tag="PKO06_24415"
     CDS             624..>1110
                     /locus_tag="PKO06_24415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNV72875.1"
                     /translation="MKPRYFLPYQVAWLKDRSRLKIWEKSRRIGATYTQSYEDVEDCV
                     NKAVPAVWFSSADESAAREYIEYCKHWAKLFNVAAKTLGEELFNEDKDVKTFGIEFPA
                     VGRRINALSSNPKAFRSKGGKIVLDEFAHHTDQKALWAAARPCITWGFPLRVISTHNG
                     FQ"
BASE COUNT          276 a          325 c          329 g          180 t
ORIGIN      
        1 ggccgaggcg aagaagaaga tgaacaagct cgatgccgat gcatccggcc gcatcatcct
       61 gctgcgcacg ctgttgagtc cctacgaaga ggatgtcacc aggctcgacg gcgagaagat
      121 caagaacgag gcaaccgagc tgtcgcgcat catcgacgag atgcgggtgc agaaagaccg
      181 catcagccgc atggagaggg acctcgaata atggcgaaga aacaggcata tttcgccgaa
      241 gccgagcggc tctacgtcgt cgaacagatg accctgaccg agatcgccgc gcgcctcaag
      301 ctctgcgagc gcaccgtgcg cacctggaag gacgaaggca gctgggaaac ccgccgcgct
      361 cagttcatgg cttcaaagga atcgttccac accgaactgt atgactttgc ccgcaagctg
      421 atgcgaacca tcagagacga catcgacgcc aaagaaaagg tcgatgccgg gcggctctat
      481 accttcgcca ggctgctgcc tcggctgacc aagatcaagg aatacgaaga cgtggtcggc
      541 ggcgagaagc ccaaagagca gaaaccgctc gatcagaaag agcttgttga cgccgtgcag
      601 aaagcgcttc tcggcgagta atcatgaaac cacgctactt tctgccgtat caggtcgcct
      661 ggctcaaaga ccgatcgcgc ctcaagatct gggaaaagtc ccggcgcatc ggggcgacct
      721 atacgcagtc gtatgaagac gtcgaagact gcgtcaacaa ggctgtacct gccgtctggt
      781 tctcgagtgc cgatgagagc gccgcgagag agtatatcga atactgcaag cactgggcga
      841 agctgttcaa cgtcgcggcg aagacgctgg gcgaagagct gttcaacgaa gacaaggatg
      901 taaagacctt cggcattgag ttccccgccg tcggtcgccg catcaatgcg ctcagcagca
      961 acccgaaagc gttccgctcg aagggcggca agatcgtgct cgacgagttc gcgcatcaca
     1021 ccgatcagaa agcgctctgg gccgccgccc gaccctgtat cacctggggc tttccgctcc
     1081 gcgtcatttc gacgcacaac gggttccagg
//
LOCUS       DAORHD010001680         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_194447_length_1108_cov_2.705603, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001680 DAORHD010000000
VERSION     DAORHD010001680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194447_length_1108_cov_2.705603"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKO06_24420"
     CDS             complement(<1..1051)
                     /locus_tag="PKO06_24420"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HNV72876.1"
                     /translation="MAHESFEDAEVASLLNQTFVCIKVDREERPDIDHIFMNVCQILT
                     GGGGWPLSIFLLPDRRPFFAGTYFPRHSRFGRVGMIELIGRINEALHRNPGQLEKAAD
                     DIVRALQQISADAPPDRPLTSATLDLAFKQFVGSFDRELGGFGKAPKFPTPHNLLFLL
                     RYGHGTGNPTATAMAEKTLISMSRGGICDQIGFGFHRYSVDSEWLVPHFEKMLYDQAL
                     LAYTFVEAYQSTRHHDWSETAKQIFTYVLRDLRSPQGTFYSAEDADTEGEEGRYYVWT
                     ESELARILTESDLQLVKTLLTVTEKGNFVEIGHSTTPGRNVLAVVRPRDETANLLELT
                     TDQLHTRWENIRERLF"
BASE COUNT          223 a          307 c          345 g          233 t
ORIGIN      
        1 caaagagcct ctcccggatg ttctcccagc gggtgtgtaa ctggtcggtc gtcagttcga
       61 gcaggttcgc ggtttcatcg cgcggacgga ccacggcgag cacatttcgt cccggtgtgg
      121 tgctgtgtcc gatctcaacg aagtttccct tctcggtcac ggtcagaagc gtttttacca
      181 gctgcaggtc ggactcggtc agaatgcgcg cgagctccga ttccgtccag acgtaatatc
      241 gtccttcttc gccttccgtg tcggcatcct cggcggaata gaaggttccc tgcggtgatc
      301 gtaggtcacg cagaacatag gtgaagatct gctttgccgt ctcggaccag tcatgatgac
      361 gagtcgactg gtacgcttcg acaaaggtgt aagccagaag ggcctggtca tacagcatct
      421 tttcgaagtg gggaacgagc cattccgagt cgaccgagta gcgatggaag ccgaacccga
      481 tctggtcgca gatgccgccg cgactcatgg aaatcagggt cttttcggcc atcgccgtgg
      541 cggtgggatt gccggtgcca tgcccgtacc gcagcagaaa cagcaggttg tgcggggtgg
      601 gaaacttggg tgctttgccg aatccgccga gttcccggtc gaacgaaccg acgaactgct
      661 tgaacgcgag atcgagtgtc gccgaagtca gcggtcgatc gggcggggca tcggccgaaa
      721 tctgctgcag cgcacgcacg atatcatccg cggccttttc gagttgccct gggttgcgat
      781 gaagggcctc gttgatacgc ccgataagct caatcatgcc cacgcgtccg aatcggctgt
      841 gccggggaaa atacgtgccg gcaaagaaag gtcgccggtc cggcagcaga aagatgctca
      901 gtggccaccc accaccgcca gtcaggatct ggcagacgtt catgaagatg tggtcgatgt
      961 ccgggcgctc ttcgcggtcg accttgatac agacaaaggt ctggttcaga agtgacgcga
     1021 cttcggcgtc ttcgaacgat tcgtgagcca tcacgtggca ccagtgacag gtgctgtacc
     1081 cgatcgagag aaagatcggc ttgtgttc
//
LOCUS       DAORHD010001681         1108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_194479_length_1108_cov_2.387464, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001681 DAORHD010000000
VERSION     DAORHD010001681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194479_length_1108_cov_2.387464"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKO06_24425"
     CDS             complement(<1..922)
                     /locus_tag="PKO06_24425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV72877.1"
                     /translation="MSEASLIVVAVAVLVMVCPLLGAEIQCVALLSDLEGNRERFDHF
                     LAHHRAFYLGDDRRWHLRSDAYFVFGGDAPDRFPGERHVLRELLRLKAESPDRVILIA
                     GNRDVNKLRLPLELSDVAMKTSPRKGKREYHVWCDEQKRVDSKATRLRWMLAKTMGSP
                     DAFELRRNELACETAVDQCAVSDQAVVDSYLEDACPGGLFHRYLKAACLMHRQGQTLY
                     VHAGMPQASLGHVPGERHRVARLDEWMKRLNEWYQEQLHTWETAYSSWDGSAPRPADA
                     LIAYAERWGQQPMNPFSVLYGRSTDVEGKVD"
BASE COUNT          244 a          347 c          273 g          244 t
ORIGIN      
        1 gatcgacctt tccttcgacg tcagtcgatc tgccatatag tacgctgaac ggattcatgg
       61 gctgctgccc ccacctttcc gcgtaagcga tgagagcatc tgccgggcgc ggcgcgctgc
      121 cgtcccagct ggaataggct gtttcccacg tgtgaagctg ctcttgatac cactcgttca
      181 gacgtttcat ccattcgtcg agacgagcga cgcgatgccg ctctcccggg acatgtccaa
      241 gagaggcttg cggcataccg gcatgtacgt agagagtctg tccctgccgg tgcatcaggc
      301 aggccgcttt tagatatctg tgaaacaggc ctcccgggca ggcatcttcc agatagctgt
      361 cgacaactgc ctgatcactt accgcacact gatcaacagc ggtttcgcag gcgagctcgt
      421 tgcgccgtaa ctcgaaggcg tccggggaac ccatggtctt tgccagcatc cagcgcagcc
      481 ttgtagcctt cgagtccaca cgtttctgtt cgtcgcacca gacatggtac tcgcgctttc
      541 ctttccgagg ggacgttttc atcgctacgt cagagagttc gagtggcaaa cggagcttgt
      601 tcacatcacg gttcccggcg atcagtatga cgcgatccgg agactctgct ttcagccgca
      661 acaactcgcg cagaacatga cgttctccag gaaaccggtc gggcgcatca cccccaaaca
      721 caaaatacgc atcgctgcgc agatgccagc gccgatcatc tccgagataa aaggctcgat
      781 gatgagcaag gaaatggtcg aaacgttcac ggttgccctc cagatctgag agcagagcta
      841 cacattgaat ctcggcaccc agtaagggac acaccatgac aagcacggca acggcgacaa
      901 caatcaagga agcctcactc acttgtttcc cccctcaccc gttccagaaa aacctgtcgt
      961 ttctcatcag caccatcata cgccacgtcg tgtcgattgc caactgaacc cctttttcgg
     1021 tctgacatcg ccacccaccg ctctgcgatt tgcttcttct gctggtttgt ttctcacatc
     1081 acaagcatca acaggacctt cagcgatg
//
LOCUS       DAORHD010001682         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_195258_length_1105_cov_3.490476, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001682 DAORHD010000000
VERSION     DAORHD010001682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195258_length_1105_cov_3.490476"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PKO06_24430"
     CDS             complement(<1..215)
                     /locus_tag="PKO06_24430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNV72878.1"
                     /translation="MTHQPPQPVLDKAYNSSLVEERVYAAWEAAGAFHADEKASQQKF
                     CIVIPPPNVTGMLTMGHVLNNTLQDIL"
     gene            complement(231..>1105)
                     /gene="grpE"
                     /locus_tag="PKO06_24435"
     CDS             complement(231..>1105)
                     /gene="grpE"
                     /locus_tag="PKO06_24435"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV72879.1"
                     /translation="EVLRDHQDQLARKDKEINELNMRLAAAVVTETPIKITNESSDIV
                     ASFIDFFDELDAMLSQNPNPQLQTLHKKLLERLIVPNEIQYQPVISEQYDANRHIATD
                     YFRSESFPERCIVFEVEKGYRRGDHMVKKSKVWVVQNLFNCQSCGVMQASSESRFCHK
                     CGEKIMAPNGLPADSLPDFQPTPNTYLRFAEAMLEKKDHSRARDYLTQGLKLDPSFVP
                     LLVKMADVHAHCSEFAEAIDVLKKASLIKPDPKIQEQINALGVRKTIFEQARNLNLPP
                     EEFEKLMGLLQQKN"
BASE COUNT          223 a          277 c          318 g          287 t
ORIGIN      
        1 atgaggatat cctgcagggt gttgttcaac acgtggccca tcgtcagcat gccggtgacg
       61 ttgggcggcg gaatcacgat gcagaacttc tgttgcgagg ctttctcatc ggcatgaaaa
      121 gctccagcgg cttcccatgc tgcatagacg cgctcttcga cgagagagga gttgtacgct
      181 ttgtcaagga ccggttgtgg aggttgatgt gtcatgagtg ccgtgattgg tcagtttttc
      241 tgttggagca gccccatcag tttttcgaat tcttccggcg gcagattcag gttgcgcgcc
      301 tgttcaaaga tcgtcttgcg cacgcccagc gcgttgatct gttcctggat cttgggatcg
      361 ggtttaatca gcgacgcctt cttcagaacg tcgatggctt ctgcgaactc gctgcaatga
      421 gcatggacat cggccatttt caccagcaga ggcacaaagc tcggatcgag tttcaggcct
      481 tgggtgagat agtcacgagc ccgcgagtga tctttctttt cgagcatggc ttccgcaaaa
      541 cgcaggtagg tgttcggcgt gggttgaaag tcgggcagag agtcggccgg taaaccgttg
      601 ggagccatga tcttctcgcc acacttatgg cagaatcggc tttcagagct agcctgcatg
      661 acaccacacg attgacagtt aaacaggttc tgcacgaccc agaccttcga tttcttcacc
      721 atgtgatcgc cacgtcgata gcctttctcg acttcgaaga cgatacagcg ctcgggaaag
      781 ctttcagagc ggaaatagtc ggttgcaatg tgacggttgg catcgtattg ctcggaaatg
      841 actggctgat actgaatttc gttcggcacg atcaaccgct cgagcagttt cttgtgcagc
      901 gtctgcagct gcgggttggg gttctgcgac agcatggcat cgagctcatc gaagaagtcg
      961 atgaagctgg cgacaatgtc tgagctttca ttggtgatct tgatcggggt ctcggttacc
     1021 accgcggccg ccaaacgcat attcagctcg ttgatttctt tgtctttccg ggccagctga
     1081 tcctggtggt cacgcagaac ttcct
//
LOCUS       DAORHD010001683         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_195946_length_1103_cov_2.462786, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001683 DAORHD010000000
VERSION     DAORHD010001683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_195946_length_1103_cov_2.462786"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKO06_24440"
     CDS             complement(<1..820)
                     /locus_tag="PKO06_24440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV72880.1"
                     /translation="MDSYWLIVQTFSPTIRRMLAAQGLVGLGAGIFAVLLNLYLRGVG
                     YHEQTIGRFLAVQSVAAAFASIPMGWLADRTSRRTTYLVGLAFLVAGFALIVVSYSPS
                     AVLAACIINGIGNGAMMVTIQPFLQENSRRRQRPFLFSLNFSMGLVMSIFAGLLAGWL
                     PRLFHFFQIIPAHDETGALRLTLGVGVAFLILAVWPAFRLPRGVLGLAKEPTASVPVA
                     KRKGDRGEESENAASMQSADSVAPFPLMARFMITSGLIGLGAGLIVPYFNLFFRD"
BASE COUNT          299 a          315 c          292 g          197 t
ORIGIN      
        1 agtcgcgaaa aaacaagttg aagtagggaa cgatcaaacc tgcaccgaga ccaatcaagc
       61 ccgatgtgat catgaaacgc gccatcaatg gaaacggtgc aacagagtca gcagattgca
      121 tcgaggccgc gttttccgat tcctcacccc gatccccctt tcgcttcgcc accggaacgc
      181 tggccgtggg ttcctttgcc agaccgagaa ctccgcgcgg cagtctgaat gcgggccaga
      241 cggccaggat cagaaatgcg acgccaaccc caagggttaa ccgcagcgct cccgtctcgt
      301 catgggcagg aataatctga aaaaaatgaa acagcctcgg cagccaacca gccagcaacc
      361 ctgcgaaaat gctcatcacc aggcccatcg aaaaattcaa actgaagaga aatggccgct
      421 gacgacgacg gctgttttcc tgcaggaacg gctggatcgt caccatcatc gctccgtttc
      481 cgatgccatt gatgatacag gcggccagca cagcggatgg tgaatacgag acgacaatca
      541 gtgcaaaacc ggccaccaga aaggcaagcc cgacgagata ggtagtacgt cgcgaggtgc
      601 gatcagccaa ccaacccatg ggaatcgaag caaaggccgc cgccaccgac tgcacggcca
      661 gaaaccggcc aatggtctgc tcgtgatatc cgacgccgcg aagatagaga ttcaggagaa
      721 ccgcgaagat tccagcgccc agacccacca ggccttgtgc ggcaagcatg cgtctaatgg
      781 tgggagaaaa ggtttgaacg atcagccagt atgaatccaa ggaagacctt tccagcgtta
      841 ttgcttcatg tcagtcgcct ggcgcgaggg agagcactct gttctctcat ggctcagcag
      901 ggaagtcact cagcacttct ttccggccaa agtccggggg aggctatgat atactaggaa
      961 tgtagaaggc attcaagctc aaactcatca ttcatggagg catgtccaat gaacaggctt
     1021 gtactggtac tcgtggcttt tgcgctctgc atttctccgc tgctcgcgga aaaagcgccc
     1081 tccgtcgctc agaaaaaaga act
//
LOCUS       DAORHD010001684         1102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196182_length_1102_cov_3.076409, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001684 DAORHD010000000
VERSION     DAORHD010001684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196182_length_1102_cov_3.076409"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKO06_24445"
     CDS             complement(<1..236)
                     /locus_tag="PKO06_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72881.1"
                     /translation="MKKWCLLSLCCCWAVMGERSVNAAESVPPKSSKWDVVKEYLRRD
                     REGGGRDVSMRPVATDSSTLPGSDGSANDPWAVQ"
     gene            complement(316..>1102)
                     /locus_tag="PKO06_24450"
     CDS             complement(316..>1102)
                     /locus_tag="PKO06_24450"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV72882.1"
                     /translation="PETLTHDELADLSESFNTTLEKLQELSVARTLQESLFPVAAVRI
                     GAYEVFGKSVAATELGGDYFDYFSVGDHQVMLLIGDVAGHGTGSALQMAMAKTGMHFA
                     VQIDPSPARVCERLNQMILSCMRKKQMMTFFCALVDTQSGVVRYANAGHNNPMLIRVD
                     GTIEEMGSGGYPLGVRKTVSYGEKELQVNPGDRIWCYTDGLPECRDAHDQDLGYDRLK
                     AILRSASAETALDTCDQVLRHCHDYRKGAPQGDDMTLILLKRS"
BASE COUNT          229 a          360 c          289 g          224 t
ORIGIN      
        1 ttctgtacgg cccaggggtc gttcgcagag ccatcgctac cgggaagggt cgagctgtca
       61 gtcgccaccg ggcgcatgga gacatcccgg ccaccgccct cgcggtcacg ccgtagatac
      121 tctttcacga cgtcccactt ggagcttttg ggaggaacgg actccgccgc gttcaccgag
      181 cgctctccca tcaccgccca gcaacaacac aatgaaagca ggcaccactt cttcatccgg
      241 tttctcccaa cgagcatcaa ttcgccatcg attgtaccgc cttgtgggcc acgcgtccag
      301 tccgacactc gcgggtcagg aacgtttcaa caggatcaac gtcatgtcgt ccccttgtgg
      361 tgcgccttta cggtaatcat ggcaatgccg taacacctga tcgcaggtat ctagcgctgt
      421 ctctgctgat gcagagcgaa gaatcgcttt caggcggtcg taccccagat cctggtcatg
      481 cgcatcgcgg cactccggca ggccgtcggt gtagcaccag atacgatcgc cgggattcac
      541 ctgcagttct ttctcgccat acgaaaccgt ctttctgaca ccgagcggat acccgccgct
      601 tcccatttcc tcgatcgtcc cgtcgacgcg aatcagcatc gggttgttat gaccggcatt
      661 cgcatagcgc acaacccccg actgcgtatc gaccaacgca cagaagaagg tcatcatctg
      721 cttcttccgc atgcacgaga ggatcatctg gttcaagcgc tcacacaccc tggccggcga
      781 cggatcaatc tggacggcaa agtgcatgcc ggttttggcc atggccatct gcaacgctga
      841 accggtgcca tgcccggcca catcgccgat gagcagcatc acctggtggt ccccgaccga
      901 aaaatagtcg aaatagtcgc cgccaagctc tgtcgcagcc acggatttgc cgaaaacctc
      961 gtatgctccg atccgaacgg ccgccaccgg gaacaagctt tcctgaagcg tgcgggccac
     1021 ggaaagttcc tgcagctttt cgagcgtggt attgaatgat tccgaaagat cggccaactc
     1081 gtcgtgcgtc aacgtctcgg ga
//
LOCUS       DAORHD010001685         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196466_length_1101_cov_3.663480, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001685 DAORHD010000000
VERSION     DAORHD010001685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196466_length_1101_cov_3.663480"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PKO06_24455"
     CDS             complement(<1..1072)
                     /locus_tag="PKO06_24455"
                     /inference="COORDINATES: protein motif:HMM:NF021642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor SrfC family protein"
                     /protein_id="HNV72883.1"
                     /translation="MGSDAKARQGRKNLLFQVFTKFDSDLIWKKGQDPQVRWDSRLKT
                     NFEEFFGKAGTWVEDWDGVTAFQNCYWVRNPNVQQTVFGRDQQGNEFVREETQLNEIM
                     NQYLANELVRRHFKNPQESWKRAASPGHPGIPWLVENVRASVEPLAKVMQLKQNLAQI
                     LAGLKSHLQPHFVGDDVAKARQQAEERARKRLAALSKEMAFKYSLPQILDRERFSISD
                     RLVSMVFDRVVNPMNDEAEAASTGGVAQPEAQVFEADIFAVGGDTPAAATPAVPSKAP
                     PRKGETFAQGVLNLWQEQLVRLAKDKTLQQTTGLDADWFAEVTQEIIKGASRLKLSED
                     LAALSETFLNAPNPGRFVRKTGV"
BASE COUNT          216 a          347 c          289 g          249 t
ORIGIN      
        1 gcactccggt cttgcgcaca aatctcccgg gattcggagc attcaggaag gtttcgctca
       61 acgccgcgag atcttcggaa agcttcaggc gtgaagcccc cttgatgatc tcctgtgtga
      121 cttcggcgaa ccaatcggca tcgagaccgg tcgtctgctg caacgtctta tccttggcca
      181 ggcgcaccaa ttgttcctgc cacagattga gcactccctg ggcaaaggtc tcgcccttgc
      241 gcggcggtgc cttgctcggt accgccggag ttgcggccgc gggggtatca ccaccgacag
      301 caaagatgtc cgcttcgaac acctgggctt cgggttgcgc cacgccgccg gtcgaggcag
      361 cctcggcttc atcattcatg gggttcacaa cgcggtcaaa aaccatactg accagtctgt
      421 cagagatcga aaaccgctcg cgatcgagaa tctgcggcag agaatacttg aaggccatct
      481 ccttgctcaa cgccgcgaga cgtttgcggg cgcgttcttc ggcctgctgt ctggctttcg
      541 ccacgtcgtc gccgacgaaa tgcggctgca gatgactttt cagaccggcg agaatctgcg
      601 ccagattctg tttcaactgc atcacttttg ccaatggttc gaccgaggca cgcacgttct
      661 cgaccaacca cggaatgccg ggatgcccgg gagaagcagc gcgcttccag gattcctgcg
      721 ggttcttgaa gtgacggcga accagttcat tcgccaggta ctggttcatg atctcgttca
      781 gctgcgtctc ttcgcgtacg aactcattgc cctgctgatc tcgcccaaag acggtctgct
      841 gcacattcgg attgcgcacc cagtaacagt tctgaaacgc ggtgacgccg tcccagtcct
      901 caacccaggt gccggcttta ccgaaaaact cctcaaagtt ggtcttcaga cggctgtccc
      961 accgaacctg cggatcctgc ccctttttcc agatcagatc actgtcgaac ttggtgaaaa
     1021 cctgaaacag caggtttttc ctgccctgac gcgccttcgc atccgatccc atgctccatt
     1081 cgacccactt gttgatcatg t
//
LOCUS       DAORHD010001686         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196524_length_1101_cov_2.727533, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001686 DAORHD010000000
VERSION     DAORHD010001686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196524_length_1101_cov_2.727533"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKO06_24460"
     CDS             complement(<1..839)
                     /locus_tag="PKO06_24460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72884.1"
                     /translation="MGSSVLATGALTIDDTNGAGGGSIVSQLVEDLAQALMRGWYAYL
                     AVLVWLILCERLTSKRVRVTGSARARLALSVFEAVTGALILFLLFPKNTLQFGEEYAT
                     WLSFIVLPHFLLPGLAVCAAWTVRRHPLLMLESFPFFCLVWPLTLFAVDYHEYPMHLY
                     SAIDFHQGLPCICAAHAGVSRWPYYLLRTLMQMCESTMHGMVSATALYGLSSLILRVP
                     GSEYARQANSEKTVLVEKWLWRAVIWFWAVFVLWIAVGLSAWFFGVDTRWFIPLLLLV
                     PAI"
     gene            complement(840..>1101)
                     /locus_tag="PKO06_24465"
     CDS             complement(840..>1101)
                     /locus_tag="PKO06_24465"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV72885.1"
                     /translation="PLDYAGDTGTVYDTTGLEQICMLGSYMNQLPTPEALKALDALVR
                     RDMKALKFTRDQIFAHRELAQTDCPGHELYKWYKDVFKETLP"
BASE COUNT          295 a          309 c          295 g          202 t
ORIGIN      
        1 catatagcgg gcacaaggag caagagtgga atgaaccagc gcgtatcgac cccgaaaaac
       61 caggctgaca agccgacggc gatccacaga acaaacacgg cccaaaacca gataaccgcc
      121 ctccacaacc atttctcgac cagtacggtt ttctcactgt tcgcttgtcg cgcatactct
      181 gagccgggca cccgaagaat cagagaggac aagccgtaca atgcggtagc agaaaccatt
      241 ccgtgcatcg tcgactcgca catctgcatg agcgtgcgaa gaaggtagta tggccagcgc
      301 gaaactcctg catgagcggc acagatacat ggcaatcctt gatggaagtc gatcgcggag
      361 tagaggtgca tcggatactc gtggtaatcg accgcaaaca gagtaagtgg ccagaccaga
      421 cagaagaacg ggaatgactc gagcatcagc agaggatgac ggcgaacagt ccatgccgcg
      481 caaaccgcca gtcccggaag aagaaaatgc ggcagaacaa tgaacgagag ccacgtagca
      541 tattcttctc cgaactgtag cgtgttcttc ggaaacaaca ggaaaagaat caacgcacca
      601 gtcaccgcct cgaaaacgga aagagcgagt cgcgcacgcg ccgaccccgt cactcgcact
      661 ctcttgctgg tcaatctttc gcacagaatc agccagacga gaacagccag ataagcatac
      721 caaccgcgca tcagagcttg tgccagatct tctacgagtt gtgacacaat acttcccccg
      781 cccgcgccgt tcgtgtcatc gatggtgagc gccccagtcg ccagtaccga gctacccatt
      841 cacggcagtg tttctttgaa cacatctttg taccacttgt acagttcgtg acccggacag
      901 tcggtttgtg caagttcacg atgggcgaag atttgatcgc gagtgaattt caaggctttc
      961 atatcgcggc gcaccagggc atcgagagct ttcagagctt cgggcgtggg cagctgattc
     1021 atgtaactgc cgagcatgca gatctgttcg agaccggtgg tgtcgtagac cgtgcctgta
     1081 tcgccggcgt aatcgagcgg g
//
LOCUS       DAORHD010001687         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196531_length_1101_cov_2.630019, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001687 DAORHD010000000
VERSION     DAORHD010001687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196531_length_1101_cov_2.630019"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..>1101)
                     /locus_tag="PKO06_24470"
     CDS             complement(75..>1101)
                     /locus_tag="PKO06_24470"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV72886.1"
                     /translation="ENSYWMALVPHPAEGVPATIYIDRNRDGDFLNDNPPLYRGTGTS
                     RSAAQIQIKYRRRDGSSFPLHQWLWTSVEWPLVAKPHWFNFYTQCWKQGTLSLPENAE
                     VAARNDEYQVWAKDPENTGDFANQRVVIDWNRNKKTEEPEWLEIGKTVRMGEHEIRLV
                     SISPYSDEVCFAVRQLPTAERTVQDVQMTLSQEPIPGNVPPPLDVHDLSGRRISLGNY
                     RGKVVLLDFWATWCGPCLRKLPEIKGLRQTFPESELVIIGISLDKDLDKLRQFCTAED
                     LTWPQHCDGEGWKNDVVRRYRVQGIPALFLIGRDGVIRAVNPRESQIHREIKRHPSPV
                     KKSGSNR"
BASE COUNT          226 a          305 c          303 g          267 t
ORIGIN      
        1 ccgcttgaga tcaaagtgag tcatgggaag aaggtacctc cttgatttcc gcggagttga
       61 tgattgaact gaattcagcg gttagagcca ctttttttca ccggggaagg atgccttttt
      121 atctcacgat ggatctgaga ttctctggga ttaactgccc gaatcacacc gtcacggccg
      181 atcagaaaca gagcgggaat accctgaacc cggtatcgcc tgacgacatc gtttttccat
      241 ccttcgccgt cgcaatgctg gggccatgtt aaatcctcgg ccgtgcagaa ttgtcgaagc
      301 ttgtccagat ccttgtcaag actgatgccg atgatgacca gttcgctctc cggaaacgtc
      361 tgccgcagac ccttgatttc aggaagcttg cgaagacatg gaccgcacca ggttgcccag
      421 aagtccagca acacgacctt tccccgataa ttgccgaggc tgatcctacg cccggaaaga
      481 tcgtggacgt cgagtggagg gggaacgttg cctggaatgg gctcctggct cagtgtcatc
      541 tgaacatctt gaacggttcg ttccgcggtc ggcagctgtc tgacagcgaa acagacctcg
      601 tcggaatatg gactaatact cacgaggcgt atctcatgct ctcccattcg cacggttttc
      661 ccaatctcga gccactctgg ttcctcggtc ttcttgttgc gattccagtc gatgacgacc
      721 cgttggttgg caaaatcacc cgtgttttcc ggatccttgg cccacacctg gtattcgtcg
      781 ttcctggccg cgacctcggc attttccggc agagagagag ttccctgttt ccagcattgg
      841 gtgtagaagt tgaaccaatg gggctttgcg accagcggcc actcgaccga cgtccacaac
      901 cattgatgca gggggaagga acttccgtcc cgccgtcgat atttgatctg gatctgcgcc
      961 gccgagcgcg atgtcccggt tccgcgatag agcgggggat tgtcgttgag gaaatccccg
     1021 tcgcgatttc ggtcgatgta gatcgtggca ggcactcctt cggcaggatg cggcacgagg
     1081 gccatccagt atgagttctc t
//
LOCUS       DAORHD010001688         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196595_length_1101_cov_2.240918, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001688 DAORHD010000000
VERSION     DAORHD010001688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196595_length_1101_cov_2.240918"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PKO06_24475"
     CDS             complement(<1..1063)
                     /locus_tag="PKO06_24475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72887.1"
                     /translation="MHLRNTVSARTVTIAEQAETLRRSEEDLRITLHSIGDAVISTDT
                     DGRIVRMNEVAEKLTGWSQAEAVGKPSTDVFRIVQGEARQQCANPVEKVLASGEHVEL
                     ANHTVLVSRTGREYQIADSGSPIRDRDGQIVGVVLVFRDVTEQYVLEERLRQSQKLEA
                     VGQLAGGVAHDFNNMLGGIIGGAELLEMGMTPNDEQRKALDIIINAAERARELTRKLL
                     VFSRKGRLNLVPIDVHVAIRETVQILERTIDKRIKIEVALDAADSTINGDISQIQNAL
                     LNLGINASHAMPEGGTIRFATRNTVIEDLFCRTNTFDLKPGPFVDIEVRDTGCGIVPE
                     NLKRVFEPFFTTKGVGKGTG"
BASE COUNT          220 a          316 c          307 g          258 t
ORIGIN      
        1 gacccgttcc cttgccgacg cccttggtcg tgaagaatgg ttcgaacacg cgtttgagat
       61 tctcggggac gataccgcac ccggtatcac ggacctcgat atcgacaaat ggcccaggtt
      121 tcagatcaaa cgtattcgta cgacagaaga ggtcttcgat cacagtgttt cgtgtggcaa
      181 aacgaatagt accaccttca ggcatggcgt gcgaagcatt gatgccgaga ttcaacagcg
      241 cattctgaat ctggctgata tcgccgttga tcgtcgagtc ggccgcgtcg agtgcgactt
      301 cgattttgat gcgcttgtcg atcgtccgct cgagaatttg aaccgtttcg cgaatggcca
      361 cgtgtacatc gatgggaacc agattgagtc ggcctttgcg cgagaagacc agcagttttc
      421 gtgtcagttc gcgtgcccgt tcagccgcgt tgatgatgat gtcgagcgct ttccgctgtt
      481 cgtcgttcgg ggtcatgccc atttcaagaa gttccgcccc tccgatgata ccgcccagca
      541 tgttgttgaa gtcatgtgca acaccaccgg ccagttgccc gaccgcttcc agcttctggc
      601 tctggcggag tcgttcttcg agcacatact gttcggtgac atcgcgaaag acgagcacca
      661 cgccgacgat ttgaccatcg cggtcgcgaa tgggtgatcc gctgtcggcg atctgatact
      721 ctcggcccgt gcgtgaaacg agaacggtgt gatttgccag ttcgacatgc tcgccgctgg
      781 ccagcacctt ttcgaccggg ttggcacact gctgccgcgc ttccccctgg acaatgcgaa
      841 agacatcggt cgacggcttg ccgaccgctt ccgcctgtga ccagcccgtg agtttttcgg
      901 caacctcgtt catgcgaacg atgcgaccgt ccgtatctgt cgagatcacc gcatcgccaa
      961 tcgaatgcag ggtgatccgc agatcctctt cgctccgtcg gagtgtttcc gcctgctcgg
     1021 cgatggtcac ggtgcgggca ctgacggtat tgcgcagatg caagctgagt atcaagcaca
     1081 aaaaatgaca cgaactattg a
//
LOCUS       DAORHD010001689         1101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196620_length_1101_cov_2.085086, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001689 DAORHD010000000
VERSION     DAORHD010001689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196620_length_1101_cov_2.085086"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKO06_24480"
     CDS             <1..548
                     /locus_tag="PKO06_24480"
                     /inference="COORDINATES: protein motif:HMM:NF021926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HNV72888.1"
                     /translation="VSEAFKVTGASGNTIETLDWKPAFDVYRQVVERHDGRSFDHTPF
                     FDVAKAYPFGIAKYGAEMVVRDPLMIKEKSLVCVGRVPQESFVHILHGSPESLIKAAG
                     KARDLAYASLRGAEPSAMIFIDCISRVLFLGTEFDRELQTVCLEGLPMVGALTIGEIA
                     NCGHEFLEFYNKTSVVGLLSS"
     gene            624..926
                     /locus_tag="PKO06_24485"
     CDS             624..926
                     /locus_tag="PKO06_24485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HNV72889.1"
                     /translation="MYVTLVKVVVRSLMVAEFIEACRVNHEHSIHEPGNCRFDILQSA
                     DDPTKFVLYEAYESEEAAMAHKKTSHYLQWRERVADMMAEPRRGDVYVGLYPLGTR"
BASE COUNT          218 a          274 c          348 g          261 t
ORIGIN      
        1 aagtttctga ggcgttcaag gtgacggggg cctcgggaaa caccatcgaa acccttgact
       61 ggaagccggc atttgacgtc tatcgccagg tggttgagcg gcacgacggg cgttcgttcg
      121 atcatacgcc gtttttcgac gttgccaagg cgtatccgtt cgggatcgcc aagtatggtg
      181 ccgaaatggt ggtccgtgat ccgctgatga tcaaggaaaa gagtctggtg tgcgttggtc
      241 gggtcccgca ggaatcattt gtccatatcc tgcatggcag ccctgaaagc ttgatcaagg
      301 ccgcgggcaa ggcgcgggat ctcgcgtatg cctcgttgcg gggagcggag ccctcggcga
      361 tgatcttcat cgactgcatc tcccgggtgc tgtttctcgg gacggagttc gaccgtgaac
      421 tgcagacggt gtgtctcgaa ggacttccca tggtcggcgc attaaccatt ggtgagatcg
      481 ccaactgtgg gcatgagttt ctcgagtttt acaacaagac gtccgtggtt gggctgctgt
      541 catcgtgagt gtctcgttcg atggcctgtc gcggttgcat cgacaacgaa gatcgaaggg
      601 gaacgcgatc gaaggaggag atcatgtatg tgacactggt caaagtcgtc gttcgatctc
      661 tcatggtagc tgagttcatc gaagcctgtc gggtaaatca tgaacactcg attcacgaac
      721 cgggtaactg tcgctttgac attctgcaat ctgccgatga tccgacgaaa ttcgttttgt
      781 acgaagccta cgagtccgaa gaggcggcca tggctcacaa gaaaacctcg cactatctgc
      841 agtggcgtga gcgggtggct gacatgatgg ccgagccgcg gcgtggcgat gtctatgtcg
      901 ggttgtaccc gttgggaact cgttaagtga gcctatgaga agctttagcg cgggaaggat
      961 tccgcgcatc gtgtttcgtg atggcgccgt gacatccctg gtggatgaga tcaggccgta
     1021 tggccgtcgg gtactgctgc tcaccggcaa gcgctcattc tgtcacggac cttcgtggcc
     1081 gtcgacctgt gaacagcttc g
//
LOCUS       DAORHD010001690         1100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_196871_length_1100_cov_2.667943, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001690 DAORHD010000000
VERSION     DAORHD010001690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196871_length_1100_cov_2.667943"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..>1100
                     /locus_tag="PKO06_24490"
     CDS             223..>1100
                     /locus_tag="PKO06_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitropropane dioxygenase"
                     /protein_id="HNV72890.1"
                     /translation="MHTATNAPVSPGSSASALYQRQSVGVWHGAVDSLAPVETHLAAQ
                     LQRLSEPCFLTLRDGVPMVSAQGSATLGTLPGHDEGFPLHAWLNPVALEDLGDKQFCR
                     DLGIRFPVVGGSMAHGISSPEIAEALGRQGMISFIGTAGQSPASVEQLILRMKHLNPA
                     VPFGFNLIHSPNETGLEETLVELYIRHDLHLIEASAFLTVTLPLVRYRLHGIHRAPDG
                     RIVVPNRIMAKVSRIEVAAKFFAPPPEKMVQELVQRGVLTSEQAQLAAQIPLAQDITA
                     EADSGGHTDNRPAVTLL"
BASE COUNT          189 a          341 c          339 g          231 t
ORIGIN      
        1 gattcagtat ggatggttga cccgtggtcg gcgtcgatat cgctgtcccg ggatcgaggt
       61 gcaggacatc atcgcccaga agcactgaag gggcgtgggc ctgcgactgc tttccatctc
      121 gtccgatgat ggcaccgggt tgtgtttttg tcagctgacg ggtacactcg gcgccttgaa
      181 gatttcgttc cgattacgtc atcaggagtg aggctgacca tcgtgcatac cgcaacaaac
      241 gcccctgttt ctcccggctc ttccgcatcc gctctgtatc agcgtcagtc cgtcggagtc
      301 tggcacggcg cggtcgactc gctggcgccg gttgagacac atctggcagc acagctgcag
      361 cgcttgtccg aaccctgctt tctgacgctg cgtgacggag tgccgatggt gtcggcgcaa
      421 gggtcggcga cgctcggtac tctgcccggc catgacgaag gcttcccgct gcatgcctgg
      481 ttgaacccgg tggcgctcga ggacctgggc gacaagcagt tttgtcgcga tcttggcatc
      541 cgctttcccg tggtcggcgg ctcgatggcg catggcatca gttcgcccga gatcgccgaa
      601 gcgctcggcc gacagggcat gatttcgttt atcgggaccg ccggtcagtc gccggcttcc
      661 gtggaacagc tgattctgcg catgaagcac ctgaatccgg ccgtgccgtt tgggttcaac
      721 ctgattcaca gccccaacga aaccggcctc gaagaaacac tggtggaact gtacattcgt
      781 catgacctgc atttgatcga ggcctcggcc tttctcaccg ttaccctgcc gctggtgcgc
      841 tatcgcctgc acgggattca ccgtgccccg gacggtagga tcgtcgtgcc gaaccgcatc
      901 atggccaaag tgtcgcgcat cgaagtcgcg gcgaagttct ttgccccgcc gccggaaaag
      961 atggttcagg aactggttca acgcggagtc ctgacctctg aacaggcaca gctggcggcg
     1021 cagattccgc tggcccagga catcacggcc gaagccgact cgggcgggca tacggacaat
     1081 cgcccggccg tcacgctgct
//
LOCUS       DAORHD010001691         1099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_197096_length_1099_cov_4.587165, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001691 DAORHD010000000
VERSION     DAORHD010001691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197096_length_1099_cov_4.587165"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKO06_24495"
     CDS             complement(<1..226)
                     /locus_tag="PKO06_24495"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNV72891.1"
                     /translation="MACNADQLQKYLDSDLDTAELASFQDHLRTCSSCRARVERQQQR
                     EKLIRDRVRAGLPLGQLVPQVMPRLTPETAP"
     gene            complement(216..785)
                     /locus_tag="PKO06_24500"
     CDS             complement(216..785)
                     /locus_tag="PKO06_24500"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV72892.1"
                     /translation="MTEDVRSDAELVRLILQGESQRFQSLVERYERLMYSYLLPQVHH
                     WQEVEDIAQEAFLKAFRHLNSFDTARKFSAWLLKIARNILIDRYRKNAQNRREAEAYA
                     TFLQRFARTSETNTAQVVEKDDEFRRLFLDVFDLPEEFRVPLLLRIVEELSYEEIADI
                     IDAPLQTVKNRIFKARRQLREKWERSDGL"
     gene            complement(822..>1099)
                     /locus_tag="PKO06_24505"
     CDS             complement(822..>1099)
                     /locus_tag="PKO06_24505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72893.1"
                     /translation="GGGTGGKLPELHAFLIAMNSSGDCRVKTDNPFHLTGGIATDNIE
                     DLVKVGGIIEWGFQRQDVSVSKGNYSKQQCFYGLAMGPRDIEVVSED"
BASE COUNT          240 a          319 c          293 g          247 t
ORIGIN      
        1 tcggagcggt ttcgggagtc agacgaggca tcacctgggg aaccagctga cccagcggga
       61 gaccagcgcg cacccggtct cgaatcagtt tctcgcgctg ttgctgccgc tcaacgcggg
      121 cacggcacga cgaacaggtg cgcagatgat cctggaaact cgccaactcg gctgtatcca
      181 ggtcggaatc gagatacttc tgcagttgat cggcattaca ggccatcaga acgctcccac
      241 ttttcacgca gttgccggcg ggccttgaag atacggtttt tcacggtctg caacggagca
      301 tcgatgatgt cggcaatctc ttcatacgac agttcttcga cgatacgaag cagcagggga
      361 acccgaaact cttcggggag atcgaacaca tcaaggaaca agcggcgaaa ctcatcgtct
      421 ttctcgacca cctgagccgt attcgtttcg ctggttcgcg caaaccgctg cagaaaagtc
      481 gcgtaggctt cggcttcacg gcgattctgc gcattcttgc ggtagcgatc aatcaggata
      541 ttgcgggcga tcttcaacaa ccaggccgaa aacttgcgag ccgtatcgaa tgagttgagg
      601 tgacgaaagg ctttcaggaa tgcttcctgc gcgatgtcct cgacttcctg ccagtggtgc
      661 acctgcggca gaagatagct gtacatgagc cgttcatacc tttcgaccag cgattggaag
      721 cgctgcgact cgccctgaag aatcaggcgt acaagttcgg catcgcttct cacgtcttcg
      781 gtcatctcat tctaccgacc gttttcgaaa aagtttcacg gtcaatcttc tgagacgact
      841 tcgatgtccc gcggtcccat cgccagaccg tagaagcact gctgcttgct gtaatttccc
      901 ttggagacac tgacatcctg ccgttgaaag ccccactcga tgatgccgcc taccttcacg
      961 aggtcttcga tgttgtcggt ggcaatccca ccggtcagat gaaacgggtt atccgttttc
     1021 acccggcagt cacctgaaga gttcatcgcg atcaggaacg catgcagctc tggaagcttg
     1081 ccgccggttc ctcctccga
//
LOCUS       DAORHD010001692         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_197467_length_1098_cov_3.345158, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001692 DAORHD010000000
VERSION     DAORHD010001692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197467_length_1098_cov_3.345158"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..619)
                     /gene="hpt"
                     /locus_tag="PKO06_24510"
     CDS             complement(77..619)
                     /gene="hpt"
                     /locus_tag="PKO06_24510"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNV72894.1"
                     /translation="MHPDISRVLIDEQTIQSRIAELGKQISSDFKGEELTLVCILKGA
                     FLFMADLCRHIDLPLNTEFMSISSYGHSTVSSGMVRILKDLNESIEGKNVLVVEDIVD
                     TGLTLSYLVELLGTRKPKRLKLCSLTNKPQCHKKPLTVDYVGFTLPSEFVVGYGLDYR
                     DYYRNLPYIGILKPHVYEHS"
     gene            complement(625..>1098)
                     /locus_tag="PKO06_24515"
     CDS             complement(625..>1098)
                     /locus_tag="PKO06_24515"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019987177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV72895.1"
                     /translation="AIMMAYHTYHQVDTQIVRIFNTYGPRMRLNDGRVVPAFISQALA
                     GEPLTIFGDGSQTRSFCYVSDLVDGLIRLMEKGDHQPVNIGNPVEITILEFAERIRDL
                     FGKGCTIAYRPLPADDPKRRKPDITRARTLLGWEPKVALSEGLAQTVSWAKEVVK"
BASE COUNT          240 a          313 c          309 g          236 t
ORIGIN      
        1 gaccagatac gcattggttt cgagcggccc gagcggatgc gtttcgacga tcagatccgt
       61 gtccgcgtgg ccggaatcag gagtgctcat agacgtgtgg tttgagaatg ccaatgtacg
      121 gcaagttgcg gtagtagtca cgatagtcca ggccgtaccc gacgacaaac tcgctgggaa
      181 gcgtaaagcc gacgtagtcg accgtcaacg gtttcttgtg acactgcggc ttgttcgtca
      241 gggaacacaa cttcaggcgc ttcggtttgc gcgttccgag caactcgacc agatacgaca
      301 gcgtcagccc cgtatcgacg atatcttcaa cgaccagcac gttctttccc tcgatgctct
      361 cgttcagatc tttcagaatg cgcaccatgc ccgaactcac cgtcgaatga ccgtaactgc
      421 tgatactcat gaattcggta ttcagcggca gatcgatatg gcggcacagg tcggccatga
      481 acaggaacgc gcccttgaga atgcagacga gcgtcagttc ttcgccctta aagtcgctgc
      541 tgatctgctt gccaagttcg gctattctgg actgaatcgt ctgttcatcg ataagaacac
      601 gcgaaatgtc agggtgcagg gtcgtcactt caccacctct ttcgcccaac tgacggtctg
      661 cgccaggcct tcggacagag caactttcgg ttcccagccg agaagcgttc gggcacgcgt
      721 gatatcgggc ttgcggcgct tcggatcatc agcgggcaac ggacgatacg cgatggtaca
      781 gcctttgcca aacaagtcgc gaatccgctc cgcgaactcg agaatcgtga tttcgaccgg
      841 gttaccgatg ttcaccggct gatgatcccc tttttccatc aggcgaatca acccgtcaac
      901 caggtcgctg acatagcaga agctgcgggt ctgcgagccg tcaccaaaga tcgtcagcgg
      961 ctcgccggcc agcgcctgcg aaatgaaggc cggcacaacg cgcccatcgt tgagccgcat
     1021 gcgtgggccg taggtgttga agatgcgcac gatctgcgta tcgacctgat gataggtgtg
     1081 ataggccatc atgatggc
//
LOCUS       DAORHD010001693         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_197488_length_1098_cov_3.068073, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001693 DAORHD010000000
VERSION     DAORHD010001693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197488_length_1098_cov_3.068073"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1098
                     /locus_tag="PKO06_24520"
     CDS             <1..>1098
                     /locus_tag="PKO06_24520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72896.1"
                     /translation="PVSLTAGPSIGKTPTVVSWGKAVSVVFPSNSLSASQVVAIGPPT
                     HAPPIASGARILSACTISLSGAPLQSPAAIALRFDRAALPQDAVPEAIMAIRWDEQTK
                     QWETRPTSVDMKQGVAVVTTEQFSLWYLIYIAQMCVVENTENFRLVYVRPGWVETAGS
                     TYGQGETFVIQTPSVRFVPGPEAAFMVRQFLERAYAAYQQAGLPRLFAARRTQAEAAA
                     AAVLGLNLRPFTEWVFLNCNPAGFALNRQEGPAGINRRSGITGAIILGRDYDDLDDLR
                     STCAHELFHAIQNEYSNLYTQALHEWWADATADYAACRIAWNGQIELDPIAQPIGWLE
                     IPLWETAANHAYYGDHLVAHILETVPGATFL"
BASE COUNT          202 a          349 c          323 g          224 t
ORIGIN      
        1 agcctgtgtc gctgacggcc ggcccttcca tcgggaaaac gccgaccgtg gtttcatggg
       61 gaaaagcggt cagtgtcgtg tttccgtcaa actccctgtc ggcttctcaa gtggtcgcca
      121 tcggcccgcc gactcacgct ccgcccattg cctcgggggc tcgaattctg tctgcctgca
      181 ctatcagcct gagcggggca ccccttcagt cacctgccgc gatcgcactg cgttttgacc
      241 gggcagccct gccacaagac gctgttcccg aggccatcat ggccatacga tgggacgaac
      301 agaccaaaca atgggaaacc cgtccgacgt cggtcgatat gaagcagggc gtcgcggtcg
      361 tcaccaccga acagttctct ctctggtatc tcatctacat cgcgcagatg tgcgtggtcg
      421 agaacaccga aaatttccga ctggtctatg tccggccggg gtgggtcgaa acggcgggca
      481 gcacatacgg tcagggggaa acctttgtca ttcagacgcc gagcgttcgg tttgtcccgg
      541 ggcccgaggc cgctttcatg gtgcgacagt ttctggagcg cgcgtatgcg gcgtaccagc
      601 aagcgggtct gccccggctg ttcgccgctc gacgcacgca ggcagaagca gccgcggccg
      661 cggttcttgg tctgaatctg cgcccgttca ccgagtgggt ctttctgaac tgtaaccccg
      721 ccgggttcgc cctcaaccgg caggaggggc cggccggcat caatcgtcga tcaggaatca
      781 ccggcgccat tatcctgggc cgagattatg acgacctcga cgatctgcga tccacctgtg
      841 cgcacgagct gtttcacgcc atccagaacg agtattccaa cttgtacacg caggccctgc
      901 acgagtggtg ggcagacgcc accgctgatt acgctgcctg ccgcattgcc tggaacggtc
      961 agatcgaact cgatccgatc gctcaaccga tcggctggct cgagattccc ttgtgggaaa
     1021 ccgcggctaa ccatgcctat tacggcgatc atctggtagc acacattctt gagacggttc
     1081 ccggggcgac atttctgt
//
LOCUS       DAORHD010001694         1098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_197531_length_1098_cov_2.650048, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001694 DAORHD010000000
VERSION     DAORHD010001694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197531_length_1098_cov_2.650048"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /gene="gpmI"
                     /locus_tag="PKO06_24525"
     CDS             complement(<1..931)
                     /gene="gpmI"
                     /locus_tag="PKO06_24525"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNV72897.1"
                     /translation="MNKKKVLLLILDGFGLSEKTEGNAVRQANPEFLNKLFKERPLAR
                     LTASGPAIGLPAGQKGNSEVGHLAIAAGRAVQQDITRINRAIEDGTFQENPVLRQFFS
                     QTLERGRALHLIGLIGTGGIHSHQRHLCACLEAAKQAGLRQMFVHVVTDGKDTSPTAG
                     ITFVQELQAYLQKLGVGRIATISGRYYAMDRDQRWDRTEKAYLAMVHGVGNRISEPVL
                     AMEASYKANLTDEFVLPVVIEGPDGSVATINDEDSVLSFNFRADRVRQLTQALSAETF
                     TGFSRKTLNIHFLSLGVTPIWAVIAEPERPATRT"
BASE COUNT          231 a          330 c          312 g          225 t
ORIGIN      
        1 cggttctggt ggccgggcgt tccggttcgg caatgacggc ccagatcggc gtgacgccaa
       61 gactgaggaa gtgaatgttg agagttttgc gggaaaaccc ggtaaaggtt tcagccgaca
      121 acgcctgtgt cagctggcgc actcggtcag cccgaaaatt aaacgagagc acagaatctt
      181 cgtcgttaat cgtggctacg gatccatcgg gaccctcgat gaccaccggc aacacgaact
      241 catcggtcag attcgccttg taggaggcct ccatggcgag taccggttct gagatccgat
      301 tgcccacgcc gtgaaccatc gccagatacg ccttttccgt gcgatcccat cgctgatccc
      361 ggtccatggc gtaataccga ccactgatcg tcgcgatacg gcccacacca agcttctgca
      421 gatacgcctg caattcctgc acgaacgtaa tgcccgccgt gggcgatgta tccttcccat
      481 cggtgaccac gtgcacaaac atctgacgca gtcccgcttg tttcgccgct tccaggcagg
      541 cacagagatg tcgttgatgc gagtgtatgc caccggttcc gatcaggcca atcaggtgaa
      601 gggcgcgacc acgctccagg gtctgggaaa aaaactgtcg gagaaccggg ttctcctgga
      661 aggtaccatc ttcgatcgcc cggttgatgc gggtgatatc ctgctgcacc gcgcgaccgg
      721 cagcaatcgc cagatgcccg acctccgagt tccctttctg gcctgccgga agaccgattg
      781 ccggcccaga ggctgtcagt cgggccaacg ggcgctcctt gaagagcttg ttcaggaact
      841 ccggattcgc ctgacgcacg gcattccctt cggttttctc cgaaaggccg aatccgtcga
      901 gaatgagcag aagtactttc tttttattca tgatcgggaa tgtcagggca aagaagatgg
      961 cggattatag cttgcctcaa cgttcaagtc aaacgcctgc cgtagcgccc tctggcgctg
     1021 tgctctttga gttgccggcc ccgaagggca aaacccgcca ctcaggcaac cggaagggcc
     1081 cgtgaatgcg acccgggt
//
LOCUS       DAORHD010001695         1096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198184_length_1096_cov_2.697406, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001695 DAORHD010000000
VERSION     DAORHD010001695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198184_length_1096_cov_2.697406"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..>1096
                     /locus_tag="PKO06_24530"
     CDS             91..>1096
                     /locus_tag="PKO06_24530"
                     /inference="COORDINATES: protein motif:HMM:NF037273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72898.1"
                     /translation="MNGANPAMIDRLRVAIKRRLVALGLLSLPLPFWPAWWCGQEADA
                     VFEARSFSVFAPLVAEVTRNLDADIGVNRFQTGSWQYDGEWNLVTWQATAVGLCQLIL
                     RFPEQKASLAPLVERALDRLLATETRRFDTRSWGEDALAGLDGNHGHLAFLGYANVAL
                     SLWRSIEPQNRFADWNDRMSEALEKRFAASQLGLVETYPREVYPVDNTPALASLGLWH
                     RATGRGDQDLLKRLLQNFRERYRDSGSGLLFQCVRVDNGEPLDKPRASGTAFAAFWLS
                     LIDPTLSRELYQSLKESCQANFFGFTAFTEYPPSVEPGTGDVDSGPVIFGLSFSGTAF
                     G"
BASE COUNT          199 a          322 c          333 g          242 t
ORIGIN      
        1 cgtccacgcg cgtacgtgga tggcgatggc cattgccttc acgggcatcg tggtaaactc
       61 agtgcatgaa acgtcggtgg ttacaatcgt atgaatgggg ctaacccggc catgatcgat
      121 cgtttacggg tggccatcaa gcggcggctg gtcgcgctgg ggctgctgag tctgccgttg
      181 ccgttctggc ctgcctggtg gtgcggtcag gaggcagatg ccgtttttga ggcgcgatcg
      241 ttttccgtct tcgcgccgct ggtcgcggaa gtcacccgga acctcgatgc cgacatcggc
      301 gtaaatcgtt tccaaaccgg cagctggcaa tatgatgggg aatggaatct cgtcacctgg
      361 caggcaacgg ctgtcgggct ttgtcagctg atactgcggt ttcccgagca gaaggcgtca
      421 ctcgcccctc tggttgaacg cgctctcgat cgcctgctgg cgacggagac tcgaagattt
      481 gacacacggt cgtggggtga ggatgccctc gccggcctcg acggcaatca tggacacctg
      541 gcctttctcg ggtatgccaa cgtggctctc agtctctggc gcagtatcga accccagaat
      601 cgcttcgccg actggaacga tcgcatgagc gaggctctgg aaaaacgttt tgccgcgagc
      661 caactcggac tcgtcgaaac ctacccacgg gaagtatacc ccgtggacaa cacccccgcg
      721 ctggcgtcac tcggtctctg gcatcgcgcc accggacgcg gggatcagga tctgttgaag
      781 cggctgctgc agaattttcg cgaacgatat cgcgattcgg gatctggctt gttgtttcag
      841 tgcgtccgag tcgacaatgg cgaaccactt gataaaccac gtgccagcgg caccgcattt
      901 gctgcattct ggctttccct gatcgacccg acattgtcgc gagagctgta tcaatctctc
      961 aaggagtcct gtcaggccaa cttcttcggg ttcacggcat tcacggagta tccgccgagc
     1021 gttgaaccgg gcaccggaga cgtggattca ggcccggtca tctttggctt gagcttctcc
     1081 ggcacagcct tcggcc
//
LOCUS       DAORHD010001696         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198390_length_1095_cov_4.488462, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001696 DAORHD010000000
VERSION     DAORHD010001696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198390_length_1095_cov_4.488462"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PKO06_24535"
     CDS             <1..655
                     /locus_tag="PKO06_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNV72899.1"
                     /translation="YLQSDTPLTLAETASVFGEMLTFHRLMGRQTNPAVKLALLCSKI
                     EDAFATVFRQACMTRFEQKLHAARRKEGELTPETIGKFWMETNRAMFGDSVDLTDNYS
                     FWWCYIPHFIHTPFYCYAYSFGELLVLSLYARYRKEGKSFVPKYLELLASGGSDNPVN
                     LVKKMGLDIAKPSFWQDGMNIIEEMLTEAEHLAGVVAKGGVRSSASPKVKKVAGRRK"
     gene            886..>1095
                     /locus_tag="PKO06_24540"
     CDS             886..>1095
                     /locus_tag="PKO06_24540"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV72900.1"
                     /translation="MERMTFTAECENGVFVLSVSGELDIYTLPKVKQAAEMIIERGVR
                     SIVLNLSGLVHLDSTAIGFLVQLDRR"
BASE COUNT          236 a          293 c          326 g          240 t
ORIGIN      
        1 gtatctgcag agtgataccc cgttgaccct ggctgagacg gccagcgtat ttggcgaaat
       61 gctgacgttc catcgtctga tgggcaggca gaccaatccg gcggtgaagc tggcgcttct
      121 gtgcagcaag atcgaagatg ccttcgccac cgtgtttcgg caggcctgca tgacgcgctt
      181 cgagcagaaa ctgcatgcgg cgcgccgcaa agagggcgag ctgactcccg aaacgatcgg
      241 gaaattctgg atggaaacca atcgcgccat gttcggagac tcggtcgatc ttaccgacaa
      301 ctacagcttc tggtggtgct acattccgca tttcatccat accccgttct actgctacgc
      361 gtactcgttc ggcgagctgc tggtactctc gctctatgcc cgttaccgga aggaaggaaa
      421 gtcgttcgtg cccaagtatc tcgagctgct ggcttccggg gggtcagaca acccggtgaa
      481 tctggtgaag aagatgggcc tcgacatcgc caagccgtcg ttctggcagg acgggatgaa
      541 tatcatcgag gaaatgctga ccgaggccga gcatctggcc ggggtggtcg cgaaaggcgg
      601 agttcgctct tcggcgtccc caaaagtgaa aaaggtggct ggccgtcgca agtgatcagc
      661 gaatgctgat gaacagttga agtcacgtcc aaacgcccgg gtgcaggaaa accatgaccc
      721 gggcgtttgt cgtttatgac catccttccc gggcgctgat cttcagatgc cgccgtggtc
      781 actgtgcccc tcagatcctc cccgagcggg agttgcattc cgagatggaa gtacagtaga
      841 tttgaagact gtattcattc tcgatggaga gggtccgtca atcccatgga acgaatgaca
      901 tttaccgcgg aatgcgaaaa cggggtgttt gtgctctcgg tctcaggaga gctcgatatc
      961 tacaccctgc caaaagtgaa acaggctgcg gagatgatta tcgagcgagg ggttcgcagc
     1021 atcgttctga acctttccgg gttggttcac ctcgattcca cggccattgg ctttctcgtc
     1081 cagctggatc gaagg
//
LOCUS       DAORHD010001697         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198541_length_1095_cov_2.388462, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001697 DAORHD010000000
VERSION     DAORHD010001697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198541_length_1095_cov_2.388462"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..356)
                     /locus_tag="PKO06_24545"
     CDS             complement(90..356)
                     /locus_tag="PKO06_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72901.1"
                     /translation="MPKKPIHLTPDIRDSLKAVALSTGKTEEQLILEALDQYLARLEE
                     KQGLDKLLAVAGMWKGRRDLTPLKNLRSTWKLRIDQRNSQGHTP"
     gene            448..>1095
                     /locus_tag="PKO06_24550"
     CDS             448..>1095
                     /locus_tag="PKO06_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNV72902.1"
                     /translation="MPTLHWIGKDKVVNHHLDVPVRDLKALLPEFEGRVDCIYIDPPY
                     NTGNEGWDCPFAYILASLASRSSAVDVEQIVGRVLRQPFVTRHSNPLLNMSYVFTASE
                     KFQQTLESVVKGLNKAGFSQREYRAYGEEDVPVATGDDGPATGTGSGTQPLLFTGARK
                     DANNAESAFVDTAVDGDPAGFELRNDPSESPREADEEIDKIVQVAGQAHSDFEKLA"
BASE COUNT          235 a          274 c          325 g          261 t
ORIGIN      
        1 ggggtagatc cgcttgatga agtcaacggc atcaggataa ccgcgcaaga agtcgatgaa
       61 gacgtctgta tcgacgagga tgcctttctt caaggagtat ggccttggct gtttctctga
      121 tcgatgcgga gcttccaggt gctgcgaagg ttcttgagcg gcgtgagatc tcttctcccc
      181 ttccacatac ctgcgacggc aagcagtttg tcgagaccct gtttttcttc gagtctcgcc
      241 agatactgat caagtgcttc gagaatgagc tgttcttccg tttttccggt ggaaagggcg
      301 acagccttca atgaatcccg gatatccggt gtcagatgaa tcggtttttt tggcatgtga
      361 cattttatca tggaagtgct ttgaggtgtc gcgccggtgg tacaggaact gcttggttca
      421 gattccgggt tggtggatga tgcgcgcatg ccgacgttac actggatcgg aaaggacaag
      481 gtggtcaatc accatcttga cgtgcccgtc agagacttga aagcactgct ccccgagttt
      541 gaaggacgag tcgactgcat ctacattgat ccgccttaca acacggggaa cgaaggctgg
      601 gattgcccgt ttgcgtacat tctggcgtca ttggcatcac gctcttcggc cgttgatgtc
      661 gagcagattg tcgggcgagt gttgcgccag ccctttgtca cccggcattc caaccccctc
      721 ttgaacatga gctatgtgtt cacggcatcg gaaaagtttc agcagacgct ggaatccgtg
      781 gtcaagggcc tcaacaaagc cgggttcagc cagcgagagt atcgggccta cggtgaagag
      841 gacgttcccg tcgccaccgg tgatgatggc cctgcaaccg gtaccgggag cggaactcaa
      901 ccgcttctct tcacaggcgc ccggaaggat gcgaacaatg ctgaaagtgc gttcgtcgat
      961 accgcggtcg atggtgatcc ggcaggcttt gaattgagaa acgacccctc tgagtcgcct
     1021 cgggaagccg atgaagagat cgacaagatc gtacaggtgg cgggtcaggc tcacagcgat
     1081 tttgaaaagt tggcg
//
LOCUS       DAORHD010001698         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198685_length_1095_cov_1.431731, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001698 DAORHD010000000
VERSION     DAORHD010001698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198685_length_1095_cov_1.431731"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKO06_24555"
     CDS             complement(<1..486)
                     /locus_tag="PKO06_24555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72903.1"
                     /translation="MIQKNLHEKYSPMYHLGALGSGGLAISFFLYLNFLLPHESGIIS
                     FDQLWPVLTDGPSLKALWIGLCLLVVFALSAMHIALMIWNTREFGLFKKTEAYHKLRD
                     SSAEISLMVVPLTMAMTVNVLFVNSSLLIPGLWEVIEYLFPVAMTAFVAIGIYALRIL
                     GD"
     gene            complement(577..864)
                     /locus_tag="PKO06_24560"
     CDS             complement(577..864)
                     /locus_tag="PKO06_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72904.1"
                     /translation="MHQCGQKQKPHIVYLFTETVNERIKGDLRILEILEKKDEQANFN
                     LGDPSAFLNVYDPDKEAYIRQPGGGAAELSDELNFAYNLSWFENTWADTFG"
BASE COUNT          265 a          286 c          288 g          256 t
ORIGIN      
        1 gtcacccaga atacgcaagg catagatgcc aatcgcgacg aatgctgtca tcgccaccgg
       61 gaacagatat tcgatcactt cccacagacc gggaatcagc agtgaggaat tcacgaacaa
      121 cacattgacc gtcatcgcca tggtcagcgg caccaccatc aggctgatct cggcgctgga
      181 gtcccgcaac ttgtgatacg cctcggtttt cttgaacagg ccaaattcac gcgtgttcca
      241 gatcatcaac gcaatatgca tggccgacag cgcgaacacg accagcaggc aaagaccgat
      301 ccacaatgcc ttcaggcttg gtccgtcggt aagtaccggc cagagttgat cgaaggaaat
      361 gatgccgctt tcatgtggca gtaggaagtt gaggtaaaga aagaacgaga tggccaagcc
      421 ccccgatcca agcgcgccca agtgatacat cgggctgtat ttttcgtgca ggttcttttg
      481 gatcattttt gtctcctcac atccacgggt tatacctcca gccgaaaggg ctgaattcca
      541 acgaatccgg gacgccttgc agcgccccga atcaggttat ccgaacgtat ccgcccaggt
      601 attttcaaac caagacaagt tgtaggcgaa gttcagctcg tcgctgagtt ccgccgcgcc
      661 gccccccggt tgccgaatgt aggcttcctt gtcggggtcg tagacgttca ggaaggccga
      721 cggatcaccc aagttgaagt tggcctgctc gtccttcttc tctaggattt caagaatgcg
      781 caagtcgccc ttgatgcgct cgttcaccgt ctcggtgaag aggtagacga tgtgcggctt
      841 ttgcttctgg ccgcattgat gcattcttgt gctgaatggc gagcttgaaa taattttcta
      901 gtttttctcg ttgttcagtg agagggtcgc cgatatctcg cactgaggcc atgctattga
      961 agacaacacc ggtatagatg ccagttggag tttcggcaaa agtgtaagga aagattctgc
     1021 aagtaatcgg tttctcgttt tcgccgagat ggccatgaat gaaacaaaga ttgtcgatga
     1081 gaaacgggca gctac
//
LOCUS       DAORHD010001699         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198807_length_1094_cov_2.809432, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001699 DAORHD010000000
VERSION     DAORHD010001699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198807_length_1094_cov_2.809432"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PKO06_24565"
     CDS             <1..433
                     /locus_tag="PKO06_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005212822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNV72905.1"
                     /translation="QQPLGGKAQFGGQRFGEMEVWALEAYGAAHVLQELLTVKSDDVE
                     GRVQVYETIIKGKTIMKPGIPESFKVVVSELQSLCLQVDLKKDSREVTILEGGGHLLP
                     TEGESDSDSWDEEHEEDEDLTSGTEEGEDDDDGEEADDDAE"
     gene            473..>1094
                     /locus_tag="PKO06_24570"
     CDS             473..>1094
                     /locus_tag="PKO06_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV72906.1"
                     /translation="MLEMDKFDAIQISIASPDKIRSWSYGEVKKPETINYRTHKPERD
                     GLFCERIFGPTKDFECFCGKYKSIRYKGIICERCNVEITKSAVRRERMGHIQLVTPVA
                     HIWYGKGSPNYIALLLDISARDLEKVLYFQSYIVIDPGNLPLSKKQLLPEEGTNGEMG
                     YRELREKYGDMFVAKMGAEAIRELLAEIDLKKLEGELNQQLKTSTGT"
BASE COUNT          281 a          277 c          331 g          205 t
ORIGIN      
        1 gcagcagcct ctgggcggta aggcgcagtt tggtggacag cgcttcggag aaatggaagt
       61 ctgggcactc gaagcctacg gagccgcgca tgtgctgcag gaattgctga cggtcaagtc
      121 ggacgatgtc gaaggccgtg tgcaggtcta tgaaaccatc atcaagggca agaccatcat
      181 gaagcccggc attcccgagt cgttcaaagt tgtggtctcg gaactgcaga gcctctgtct
      241 gcaggtggac ctcaagaaag attcgcgtga agtcacgatt ctggaaggtg gcggacatct
      301 gctgccgacc gagggtgaaa gtgattctga ctcgtgggat gaagaacacg aggaagatga
      361 agatctgacc agtggtaccg aagagggtga agacgacgac gacggcgaag aagccgacga
      421 tgatgccgaa taatcgagca ctcggcacac agacgaagga gggaaaatag tcatgttaga
      481 aatggataag tttgatgcga ttcaaatcag catcgcgtct ccggataaga tccgctcctg
      541 gtcgtatggt gaggtgaaga agcccgaaac gatcaactat cgcacgcaca agcccgagcg
      601 cgatgggttg ttctgtgagc gcatcttcgg ccccaccaag gatttcgagt gcttctgcgg
      661 caagtacaag agtattcgct acaagggcat catctgcgaa cgttgtaacg tcgaaatcac
      721 caaatcagcg gtgcgccgcg agcgcatggg tcatattcag ctcgtgacgc cagtcgcgca
      781 catctggtat ggcaagggca gcccgaacta catcgctctg ctgctcgaca tctcggcgcg
      841 cgatctcgag aaggttctct acttccagag ttatatcgtc atcgacccgg gcaacctgcc
      901 cctttcgaag aagcagctgc tgcctgaaga aggcacgaac ggcgagatgg gctatcgcga
      961 actgcgcgag aaatacggcg acatgttcgt cgccaaaatg ggcgctgaag cgattcgcga
     1021 gttgctggcc gagatcgacc tcaagaagct cgagggcgaa ctcaatcagc agctgaagac
     1081 ctcaaccggc acca
//
LOCUS       DAORHD010001700         1094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_198896_length_1094_cov_2.189605, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001700 DAORHD010000000
VERSION     DAORHD010001700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_198896_length_1094_cov_2.189605"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="PKO06_24575"
     CDS             complement(<1..500)
                     /locus_tag="PKO06_24575"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNV72907.1"
                     /translation="MALIDPPANGGVSWLRTHVILVVIVIVSIVLFREYHDEIRDAAK
                     FLVTRFGFPMMFFLVFLADGLVQPVPPDLIVFGSGFGGANPLKAGIVAGLGSSCGGVL
                     GYLIGRLINPWTFRRLFGSKLMRNGRDLYRSYGTLTIAIAAVTPIPYSFSCYLGGLYR
                     MSLPSL"
     gene            812..>1094
                     /locus_tag="PKO06_24580"
     CDS             812..>1094
                     /locus_tag="PKO06_24580"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72908.1"
                     /translation="MLKTLARGTKGEVLATMNGWSYVRFSNGLVGWVRSDLLSSGTIT
                     PPPASDADKASLDKSFTRWDKHLKGSYLNFDNLSRRDPVSTTRKDCRSSD"
BASE COUNT          272 a          318 c          296 g          208 t
ORIGIN      
        1 aaaagtgatg gcagtgacat gcggtacagg ccgcccagat agcacgagaa gctgtaggga
       61 atgggtgtca ccgcggcaat ggcaatggtc agcgttccgt acgatctata caggtctcgc
      121 ccattgcgca tcaacttgct gccaaacagc cgacggaagg tccagggatt gatcagtcgg
      181 ccgatcagat accccagtac accaccacag gaactgccca acccggcaac gatgcctgcc
      241 ttcagagggt tcgcgccgcc gaatccgctg ccgaagacga tcaggtctgg cggaaccggt
      301 tgcaccaagc cgtcagccag aaacacgaga aagaacatca tcgggaatcc gaaccgggtc
      361 accagaaatt tggcggcatc acgaatctca tcatggtact cacggaagag gacgatcgag
      421 actataacaa tgacgaccag aatgacgtgc gtgcgcagcc acgaaacgcc gccattcgca
      481 gggggatcga tcagtgccat gacgatagac agttccttct gttcccgaga ggttattgac
      541 gagtccccag gagattcata aaatgaagac atatgccacg cctcttggag ggaccaccta
      601 tgagtatact gaaaaacgcg ctttttttcc tcctgattct ctgcctcctc tcggcggcac
      661 ccgtacaggc tcaaaccaac ccttacgggg acaatttcac agtcacgcag tcgggtcgtt
      721 ccggcacaag ttcagcaact gccgttgaca acgccgtcgc gatcggcaag cctgggtcaa
      781 tgtgcgctcc ggcccgggca cgagtcactc ggtgttgaaa accctggcac gcggcaccaa
      841 aggtgaagtg ctggccacga tgaacggctg gtcatacgtg cgtttcagta acgggttggt
      901 cgggtgggtg cgatcagacc tgctctcttc cggcacgatc actcctcctc cggcgagcga
      961 tgcggacaag gcctctctcg acaagagctt tacccgatgg gacaagcatc tcaagggttc
     1021 atatctgaac ttcgacaatc tttccaggcg cgacccagtt tccaccacca ggaaagattg
     1081 tcgaagttca gata
//
LOCUS       DAORHD010001701         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_199668_length_1091_cov_4.223938, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001701 DAORHD010000000
VERSION     DAORHD010001701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199668_length_1091_cov_4.223938"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKO06_24585"
     CDS             complement(<1..664)
                     /locus_tag="PKO06_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72909.1"
                     /translation="MSSLFLPADHQSAASRLLRLCLAVVVVWLLYCPALEAAPPGRKV
                     HREPMVRESTHVAGLTTESQTKPGAEAVSRASDDDTDTDEQVKVPRWMSFWREERAAA
                     ARRGWAWYLESSGEYSQTLKDPAGGHNRSYYHDAYLELSVDVKTAKLGTAGTAFLSLV
                     GGREHVFSAAEGASPQEDEHTPTDTNPGAGGSFQLYELWYERPVGKALWLIGLRDISS
                     DFG"
     gene            complement(723..1004)
                     /locus_tag="PKO06_24590"
     CDS             complement(723..1004)
                     /locus_tag="PKO06_24590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV72910.1"
                     /translation="MKTATASHILVATEARCLELKAQIEAGADFATVARENSKCPSGR
                     DGGSLGSFSKGDMVPEFDKVVFSQELKKVHGPIKTQFGYHLIWIESRQD"
BASE COUNT          221 a          341 c          308 g          221 t
ORIGIN      
        1 cgccaaagtc cgagctgata tcgcgcaagc cgattaacca gagagctttt ccgaccggac
       61 gttcgtacca cagctcatac aactgaaacg aaccacccgc gcccggattc gtatcggtcg
      121 gcgtgtgttc atcctcctgc gggctggctc cttcggcggc tgaaaacaca tgttccctgc
      181 cgcccaccag agagagaaat gccgttcccg ccgtacccag tttcgccgtc tttacatcca
      241 cgctgagttc gagataggca tcgtggtagt aactgcgatt gtgcccgcca gcagggtctt
      301 tcagtgtctg cgagtattcg cctgaacttt ccagatacca ggcccaacca cgacgagcag
      361 cagcggcgcg ttcttcacgc cagaagctca tccaccgcgg cacctttacc tgttcgtccg
      421 tgtcagtgtc gtcgtcactc gcccgcgaca cggcttccgc tcccggcttc gtctgcgatt
      481 cggtcgtcag gccggccacg tgagtcgatt cacgcaccat gggctcgcga tgaaccttcc
      541 ggcccggagg agcggcttcg agcgcagggc aatacagcag ccagaccacg accaccgcca
      601 gacagaggcg cagcaggcgt gatgcggcag actggtgatc ggcaggcaaa aagagtgatg
      661 acaagggtga gcagttccgg tgcagccgcg gtctcgagcg ctttccgctc tcgaggagag
      721 ccttagtcct ggcggctctc gatccagatc aggtgatacc cgaactgcgt cttgatgggg
      781 ccgtgcactt tcttcagttc ctgggaaaac acgaccttgt cgaattcggg caccatgtct
      841 cctttgctga atgagccaag actgcctccg tcgcggccgg aggggcactt cgagttttca
      901 cgagccaccg tcgcaaaatc ggcacccgct tcgatttgag ccttgagctc gaggcagcgg
      961 gcttccgtgg caaccagaat gtgagaggca gtagcggttt tcatgacaac tcctgagagt
     1021 gaaatcgaaa gagcacaaga gtatgcgtga accccgcccc cgattcaagt gtttcgttac
     1081 ccccgatggt c
//
LOCUS       DAORHD010001702         1091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_199738_length_1091_cov_2.838803, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001702 DAORHD010000000
VERSION     DAORHD010001702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_199738_length_1091_cov_2.838803"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..963)
                     /locus_tag="PKO06_24595"
     CDS             complement(172..963)
                     /locus_tag="PKO06_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72911.1"
                     /translation="MTMRRWVLMVLVCVGACQGMVFGQAIEAPFVLWMANNEARSMML
                     LAQDRQLLDRMSCPLGLLTLREMPGVRVEEALWAQHATMYRMLPEVSEVFRLARRFGS
                     GAFMRVVEQARPGYATVQAFFAGPGNMGRTLAPILDKRFQEDFLTAVGLPKDSEQDLS
                     KRLEQVLRSVRDSERRLLEQYLDNFLALYPKNRPTRYLASLVKNGLNREFAKGMTQMV
                     NEVNQAVAASPEPADAASGGDKPPAGDAVATGTEPAGEDPFAIFK"
BASE COUNT          233 a          357 c          296 g          205 t
ORIGIN      
        1 caagttccgc cagtcgcttc agcaactcgc gcagagcctc agggtcgatg tgtccggtgt
       61 tcaaccgaaa acgtgaggtg accagtcgca acatctgaag aaccggagcg gcattcgatg
      121 cggggccgat cgtacaaacg gcatcaaaca tctctgattc tccagacgtg gtcacttgaa
      181 aatggcgaac gggtcttctc cagcaggctc tgtgccggtg gcgacggcat ctcccgcagg
      241 cggcttgtca ccgcctgacg cggcatcggc cggttcaggg ctcgcggcca ccgcctggtt
      301 cacttcattc accatctggg tcatcccttt ggcaaactct cgattgaggc cattcttgac
      361 caatgacgcc agatagcgcg tcgggcggtt cttcggatac aaggccagaa aattatcgag
      421 atactgttcg agcagacgtc gttcactgtc gcgcaccgaa cgcagcacct gttcgagacg
      481 tttcgagaga tcctgctcag aatccttcgg caggcccacc gcggtcagaa agtcttcctg
      541 aaagcgcttg tcgagaatcg gtgccagagt gcgccccatg ttccccggac cggcaaaaaa
      601 ggcctgcacg gtcgcatacc ccggacgcgc ctgctccaca acccgcatga aggctcccga
      661 gccgaaacga cgagccagac gaaacacttc actgacttcc ggcagcatgc gatacatggt
      721 tgcgtgctgt gcccagagag cttcttcgac gcgcacgccc ggcatctcac gcaaggtaag
      781 cagccccaga ggacagctca tgcgatcgag cagctgcctg tcctgagcca gcaacatcat
      841 cgagcgcgct tcgttgttgg ccatccagag cacgaacggc gcttcgatcg cctgcccgaa
      901 caccatgccc tggcacgctc cgacacacac cagcaccatc aggacccagc gtcgcatcgt
      961 catttcgtca cctccagcag gcggaacacg atcttccggg tttcattgac ggcccagaga
     1021 gggcgtatga cggcaaactc cttgttcgcg gtttcgagcg cataggtcag cgtccactct
     1081 cccgcatcgc a
//
LOCUS       DAORHD010001703         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_200008_length_1090_cov_3.648309, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001703 DAORHD010000000
VERSION     DAORHD010001703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200008_length_1090_cov_3.648309"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..581
                     /locus_tag="PKO06_24600"
     CDS             141..581
                     /locus_tag="PKO06_24600"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72912.1"
                     /translation="MLAIELKILLLTGIYFVGWFLFEKVFSRLWESWGNREPVSRKTP
                     LTKAPSMASITPLDQTARYLKGLKNPDWRIRRISAVQLGDKRGTSVVQGLIEALNDPR
                     EEVSLAAGEALAKIGDPQAIAALSDHCNTLEKRMDETYERLRAA"
     gene            858..1001
                     /locus_tag="PKO06_24605"
     CDS             858..1001
                     /locus_tag="PKO06_24605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72913.1"
                     /translation="MNGLTEMVHEAMGKVVTGSAAFGLMLFTYIVAPFELVIRALFFR
                     KET"
BASE COUNT          240 a          306 c          299 g          245 t
ORIGIN      
        1 ggccgtttca ggagtagaga aaccggggct gcgcctcaga gagcgttgag aagccacatc
       61 cgggagaacc ggtaaaaagt ctcaggacca tcgccgatca tgccgatagc ttcccagaga
      121 tacattctga ggaggcatac atgctggcca ttgaattgaa aattctcttg ctgaccggta
      181 tctacttcgt cggctggttc ctgttcgaaa aagtgttctc ccggctctgg gaatcgtggg
      241 gcaaccgcga gccggtctct cgcaaaaccc cgctgaccaa ggctccctcg atggcgtcga
      301 ttactccact tgatcaaaca gcgcgatatc tcaagggtct caagaatccc gactggcgca
      361 ttcgccgcat ctcggccgtt caacttggag ataagcgcgg caccagtgtg gttcagggtt
      421 tgatcgaagc gctgaacgac ccgcgcgaag aagtcagtct ggcggctggc gaagcgctgg
      481 ccaaaatcgg cgatccgcaa gccattgccg ccttgagtga ccactgcaac acactcgaaa
      541 agcgcatgga tgaaacatac gaacgattac gggcggccta agggtatgaa cccatgacag
      601 aaaactctct tccatcgtca gagtcttcct catcatctgc tgccaggtct cccgcggctc
      661 ccaccacagg ccagctgctc gtcgcagttc tgatgagctt gttcgtctgc cctgggctag
      721 gccaccggta tctgggaaga aatcgtgcct acattacggt tctgattctg ttcttcggtt
      781 cgtttttcgt cgttgccaac acggtgtata ctgtagcgag agatttcttt tcgcgacacc
      841 caggcgaagg actggggatg aacggtctca cagagatggt gcacgaagcg atgggaaaag
      901 tggtcaccgg gtcggccgcc tttggcctga tgctcttcac ttacatcgtg gcaccgttcg
      961 aactcgtgat tcgcgccctg tttttccgaa aagagacctg atggaacatc tcacatgact
     1021 cgtcaggcat tcatcgaact cgttcgcaga cacccgcatc gcgcactggg atgcggggtt
     1081 ctgttcatgg
//
LOCUS       DAORHD010001704         1088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_200681_length_1088_cov_2.859632, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001704 DAORHD010000000
VERSION     DAORHD010001704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200681_length_1088_cov_2.859632"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..948)
                     /locus_tag="PKO06_24610"
     CDS             complement(157..948)
                     /locus_tag="PKO06_24610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV72914.1"
                     /translation="MKLGNQAEFLRRYFFALLRIPFLFSFGVAQRSHRQLIAAICRHF
                     GYFTRMQGVPTIKLKELLPDFQYGEIHDPIGTYGSPAAEEILIINQLVKRAQPKVLFE
                     IGTFTGRTTLNLAACSPEDARVLTLDLKPEDLRQTKFPMYASEELYVNKPESGTSYTS
                     SPLASKITQLYGDSAVFDFSPWRGQVDFIFIDGAHTYEYVHSDTRRALELLRNGCGTI
                     IWHDYLMVEGLTRALNELAAQEPRLKGMRHIEGTTMTIWQSGTCG"
BASE COUNT          259 a          299 c          269 g          261 t
ORIGIN      
        1 ttcaaagata tgcgcgatct gctttcgtca ctggtgacac aacttcgcca aaacgtcggc
       61 aaagtttctc cctaatcgtt gctgagctcg agatcgtgcg ggctcattac agcccctcag
      121 tcatcgcatc ggaaacaccc gatcaggccc acttcatcaa ccacaggttc cgctttgcca
      181 gatggtcatg gttgtgccct cgatgtgcct catccccttc agtcgaggct cttgcgccgc
      241 caattcgttc agcgcacggg tgagcccttc aaccatcagg taatcatgcc agatgatggt
      301 tccgcagccg ttgcgcagca gttccaaagc acggcgggta tcgctgtgaa catactcgta
      361 ggtgtgcgca ccgtcgataa agatgaaatc aacctgacct cgccagggag aaaaatcgaa
      421 caccgcggaa tcaccataca gctgagtaat cttgctggcc aacggcgagg aagtatagct
      481 ggttcctgat tcaggtttgt tcacatacaa ttcttcactg gcgtacatgg ggaacttcgt
      541 ctgtcgaaga tcttcaggct tcagatccaa cgtgaggacc cgggcatctt caggcgaaca
      601 cgctgcgagg ttgagggtcg ttcttcccgt gaaggttccg atctcaaaca gaactttggg
      661 ctgagctctt ttcacgagtt gattgatgat gagtatctct tcggccgccg gactgccgta
      721 agtcccaatg gggtcgtgaa tctcgccgta ttggaaatcc ggcagcagct ccttcagctt
      781 gatggtcgga accccctgca tacgcgtaaa atagccaaaa tggcgacaaa tagcggcaat
      841 gagctgccga tgacttcgct gcgccacccc gaaactgaaa agaaagggga tacggagcaa
      901 cgcaaagaaa tatcgcctca gaaactctgc ctggtttccc aatttcatcg ttttctcctt
      961 tgctgctacc ctttcacgag actgaataca cactttatca gaggtcggct ctcttcgatc
     1021 atttgccgtg tatcactctg acagcgatgt tgtcgcctgc agaaatggac ggaaagtttc
     1081 cgaaatga
//
LOCUS       DAORHD010001705         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_200970_length_1087_cov_3.412791, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001705 DAORHD010000000
VERSION     DAORHD010001705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200970_length_1087_cov_3.412791"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="PKO06_24615"
     CDS             <1..>1087
                     /locus_tag="PKO06_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72915.1"
                     /translation="ASEKTFHYIQRVQRLEDPVIAKVDQRLIESFLGSISAIPLLAHG
                     PNQLVDLSRTGTPRLGAFYRLTLPNHSSMYLLVLIDRRRIAQQELINSALATISRLAG
                     PDYQFGWLDLRDPTHRGLATGRPVPAAWVPFLSGYHFSTLHQTPNRLFCQTTIDRTIR
                     LFGTRRLPPIDPRVPFLRQILVISSAGALLVFLWFWSYQAFPTVPLRVQLLFLFGCIG
                     TAGILCLLSTARQYRENREQVLIREQQTRATRILEKVDSSFLPAFQTLIARYRQLLDT
                     LYEDAGNLDRACDQLRHWRRDGSLLAGLVVRRDGQTILRIPEENALGAQSALASKGEG
                     LLGHFAKRIIEVFNGQEKSSATEKSEKR"
BASE COUNT          222 a          338 c          286 g          241 t
ORIGIN      
        1 cagcatcaga aaagactttt cactatattc aacgagtaca aagactggaa gatcccgtca
       61 ttgcaaaagt cgaccagcga ttgatcgagt cgtttctcgg ttccatcagt gccattccgc
      121 tacttgctca tggcccgaac cagctcgttg acctgagccg taccggaacc ccacgcctgg
      181 gggcattcta tcgcctgacc cttcccaacc acagctccat gtatctgctc gtcctgatcg
      241 atcggcgtcg cattgcgcaa caggagttga tcaattccgc cctggcaacc atcagtcggt
      301 tggccggacc agactaccag tttggctggc tcgacctgcg cgaccccaca catcgcggtc
      361 tggcaacagg gcgtcccgtg cctgccgcgt gggtcccttt tctctcggga tatcattttt
      421 ccaccctgca ccagacccct aaccgcctct tctgtcagac caccatcgat cgtaccatcc
      481 ggttgttcgg caccagacgc ctgcccccga ttgacccgcg cgttcctttt ctgcgacaga
      541 ttctggttat cagctcagcc ggagcgctgc tggtctttct ctggttctgg agttatcagg
      601 cgttcccgac ggttccgctg cgcgtgcagt tgctctttct cttcggttgc atcggtaccg
      661 ctggaattct ctgcctgtta tcgacagccc gacagtatcg ggaaaaccga gaacaggtct
      721 tgattcgcga acagcagaca cgcgctacgc gaattctgga gaaagtcgac agttcatttc
      781 tgccggcttt tcaaaccctg attgcccgat accgtcagct tctcgacacc ctgtatgaag
      841 acgccggcaa tctcgatcgg gcctgcgacc aactgcggca ctggcgacgc gacggttcgc
      901 tgctggccgg actggttgtt cggcgcgatg gccagacgat cctgaggatt ccggaagaga
      961 acgcgctggg cgcccagagc gcactcgcgt caaaagggga aggactgttg ggacactttg
     1021 ccaagcgcat catcgaggtg ttcaacgggc aggaaaaaag cagcgcgacg gaaaaaagtg
     1081 agaaacg
//
LOCUS       DAORHD010001706         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_201593_length_1085_cov_3.388350, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001706 DAORHD010000000
VERSION     DAORHD010001706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201593_length_1085_cov_3.388350"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKO06_24620"
     CDS             <1..714
                     /locus_tag="PKO06_24620"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNV72916.1"
                     /translation="GPEMLDIPAEAVSSAFLPWWFDRFWRFPDELQERIRHPFVWDDT
                     GSVAHDSSTLNRLFRQVLSSRLLTATHQGAGGTEALNLTAGQLYRRLTGRGAHESLIP
                     GEPLLMTRNEYLYDVFNGDQGLLLWAITPTSPSPALHAVFPRHHGFQAIPFEALRHDL
                     EPAYALTVHKSQGSEFDRVFLVLPPVDHPLLTREILYTGVTRARSGVVLVGSREALNS
                     GMRRSIKRRSRLQLASPTT"
     gene            complement(728..>1085)
                     /locus_tag="PKO06_24625"
     CDS             complement(728..>1085)
                     /locus_tag="PKO06_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72917.1"
                     /translation="ENGPGRIGTPVFLGRQPGDDLTIVGESHGVTFDGEHFKPLPSMA
                     PLGITAKLTCAVFDDLNPANLWVGFSAASEGGVALQQGAGWLSFPLPAAVRHVAVSEP
                     FLFLATTREVVRRAKR"
BASE COUNT          210 a          354 c          301 g          220 t
ORIGIN      
        1 gggccggaga tgctcgatat tccagccgaa gccgtctcca gcgccttcct gccctggtgg
       61 ttcgacagat tctggagatt ccctgacgag cttcaagaaa ggattcgaca tccattcgtc
      121 tgggacgata ctggttctgt ggcacacgac tcgtcgacct tgaaccgcct gtttcggcag
      181 gtgctttcga gtcgactgct gaccgccaca caccagggag cgggtgggac tgaagctctg
      241 aacctgaccg ccgggcaact ctatcgccga ctcaccggcc gaggtgctca cgaatcgctg
      301 attcccggcg aaccgctgct catgacccgt aacgagtatc tgtacgatgt cttcaacggg
      361 gatcaggggt tgctgctctg ggcaatcacc ccgacctcgc ccagcccggc tctgcacgcc
      421 gtgttcccaa gacaccacgg gtttcaggcg attccctttg aagctctgcg tcacgatctt
      481 gaaccggctt atgcgcttac cgtccacaag agccagggtt ccgagttcga tcgtgtgttc
      541 ctcgtcctcc cccccgttga ccatccgtta ctgacccgtg agattctgta taccggcgtc
      601 acgcgcgcca ggtcaggcgt cgtgctggtt ggctctcgag aagccctcaa cagcggcatg
      661 cggcgttcca ttaaacgtcg cagtcggttg cagctcgcct cacccacgac atagtctttt
      721 ctgaccgtca gcgttttgcg cgccgcacaa cctctcgggt tgtggcgaga aacaaaaacg
      781 gttcggacac cgccacgtgt cgaacggccg caggcagggg aaaactgagc caaccggcac
      841 cctgctgcaa ggcgactccc ccttcactcg cggccgagaa accgacccag aggttcgccg
      901 ggttcagatc gtcaaacaca gcgcaggtca gcttcgccgt gatccccagc ggagccatgg
      961 aaggcagggg tttgaagtgc tcgccatcaa acgtgactcc atgactctca ccgacgatgg
     1021 tcagatcatc acccggctgt cgaccgagaa aaacgggagt gccgatcctg cccggtccgt
     1081 tttcc
//
LOCUS       DAORHD010001707         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_201624_length_1085_cov_3.021359, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001707 DAORHD010000000
VERSION     DAORHD010001707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201624_length_1085_cov_3.021359"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKO06_24630"
     CDS             complement(<1..486)
                     /locus_tag="PKO06_24630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72918.1"
                     /translation="MTRTLQLLLFLSLVTLLVGVCLIEARKPGPVTFLVRDDGQGVLF
                     LESIPPDYDQLTATTTLDIGVDPGPLTAASGLASGEYHIRFPYPLLASEDQLSSLADR
                     FVKDAMSSGTFFIQVEARKTRLGYLYLYRWEYGRFSEFKDLTDIAHILSVLIDPWRRV
                     IF"
     gene            590..>1085
                     /locus_tag="PKO06_24635"
     CDS             590..>1085
                     /locus_tag="PKO06_24635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72919.1"
                     /translation="MMRRIFSLFLVFQLLSVGRVTAEPMRTTSDEEYTTETAEEIATP
                     ATSLASAPADVIDAIRHVPISTDLLGGFVNTDIDFKDPAFYDKLLMATRTNIVVEDLP
                     HGYVQITKGEFSAADNGIIMPQTLEYALFKGTNQRWVLVGSWENDHPDGYWSEVEAWL
                     WEKGV"
BASE COUNT          283 a          314 c          278 g          210 t
ORIGIN      
        1 gaagataacc cgccgccacg ggtcgatcag cacgctcagg atatgcgcaa tatctgtcag
       61 atccttgaat tcagagaatc ggccatattc ccaccggtac aaatacaggt aaccgagtct
      121 tgtcttgcgc gcttcgacct gaataaaaaa cgtgcccgaa ctcatggcat ctttcacgaa
      181 gcgatcggcc aaagacgaca gctgatcttc acttgccagc aacgggtacg gaaaacgtat
      241 gtgatattcg cccgatgcca agccagaggc agcggtcaga ggccccggat caacaccgat
      301 gtccagtgtc gtcgtggccg tgagctggtc ataatcaggc ggaatcgact cgagaaacag
      361 caccccctgt ccatcgtcgc gcacaagaaa agtgactggc ccgggtttgc gtgcctcaat
      421 gagacaaacg ccaaccagta aggtcaccag tgacaggaac agaagcagct gcaaggttct
      481 ggtcatggac cgaccaatga tctgagacga accattccgc cagacacctc cccatgctcg
      541 gattcacagg gcaaaagatg agatcagaat ctatggtaac atggtgtcca tgatgcgacg
      601 aatcttctcc ctgttccttg tgttccagct gctctctgtc ggtcgggtaa ccgccgaacc
      661 gatgagaaca acttccgatg aggaatacac aacggaaacc gcggaggaaa tcgccacccc
      721 cgcaacgtct ctcgcctcgg cccccgcaga tgtcatcgat gcgatccggc atgtgcccat
      781 ctcgaccgat ctcctcggcg gatttgtcaa taccgacatc gacttcaagg accccgcttt
      841 ttacgacaaa ctactcatgg caacccgcac gaacatcgtc gttgaagatc tccctcacgg
      901 atacgtgcag atcaccaaag gtgagttcag cgcagcagac aatggaatca tcatgccgca
      961 gaccctcgaa tatgctctgt tcaagggcac aaaccaacgg tgggttctgg tgggcagctg
     1021 ggaaaatgat cacccggacg gctactggtc ggaagtcgag gcctggttgt gggaaaaagg
     1081 cgtct
//
LOCUS       DAORHD010001708         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_201660_length_1085_cov_2.674757, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001708 DAORHD010000000
VERSION     DAORHD010001708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201660_length_1085_cov_2.674757"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PKO06_24640"
     CDS             complement(<1..138)
                     /locus_tag="PKO06_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV72920.1"
                     /translation="MRIVVTGGAGFIGSHLTDRLLAEGHRVIVIDNFVTGSRRNLSPH
                     EQ"
     gene            371..>1085
                     /locus_tag="PKO06_24645"
     CDS             371..>1085
                     /locus_tag="PKO06_24645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72921.1"
                     /translation="MKPAPPSLARTALFLASVVLLLLPAVICQQALQKTEEQLSSQAA
                     ADALSNSWDMLKLLRLNSLPRLLLEERLDGIVERIADTYTDNHSGTELGRLQQAVHDT
                     LSHEFLGITTTSSLPLDGEATPPASGSVFMALGVWGFQTEGASSSRVIPLVYSGLPKE
                     YMSLFNQMMYLCIICNLGTADTVRVVSAADDLLEKIRPVIGGLYQGMYHVAYFLRGSI
                     IPGTFQGRDCWLFWQILHNH"
BASE COUNT          221 a          332 c          287 g          245 t
ORIGIN      
        1 ctgctcgtgc ggcgacaagt tgcgccgact gccggtaacg aaattgtcga tgacaatgac
       61 ccgatgccct tccgcgagca gtcggtcggt gagatgagac ccgatgaaac cggcgccgcc
      121 ggtgaccaca atgcgcatga tcgttccttt ttcacttgtg tggattgaat caattcatta
      181 cactgccaga gatgaaaggt caagcccgga actctcacca tcaggctttc tggcaactcc
      241 accagggtgt gcgccgggca gatctccccc tcacgcgtcg accttcgcac ctggtccccg
      301 tcgtcggcac tcacgaggca cagctctgcc gcagacccaa gttcagccac cccttcgcgg
      361 aaaccacggc gtgaaacccg caccgccgtc tctggctcga accgccttat tcctggcttc
      421 ggtggttctc ctgctgcttc cggccgtcat ttgtcagcag gcactgcaga agaccgaaga
      481 acagctttcc agccaggcgg cggctgatgc tctgtcaaac agttgggata tgctcaagct
      541 gctgcgtctg aactccctgc ctcgactgct gctggaagaa cgcctcgacg ggattgtcga
      601 gcgcattgcc gacacctata ccgataacca cagcggtacg gaactcggac gtctgcagca
      661 ggcggttcac gacactctgt cccatgagtt tcttggcatc accaccacaa gctctttacc
      721 cctcgatggc gaggccaccc ctcccgcctc cggatccgtg ttcatggccc tcggcgtgtg
      781 gggttttcaa acggagggtg catcgagtag tcgggtcatt ccgctggtgt attcaggtct
      841 tcccaaagag tacatgtcgc tattcaatca gatgatgtat ttatgcatca tctgcaatct
      901 gggaacagca gacacagtgc gtgtggtcag cgcagccgac gatctgctcg aaaaaatcag
      961 accggtgatc ggcggcctgt atcaaggtat gtatcatgtg gcctatttcc tgcgcggttc
     1021 gattattcca ggaacatttc aaggtcgtga ctgctggctg ttctggcaga tcctgcacaa
     1081 tcatc
//
LOCUS       DAORHD010001709         1084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_201999_length_1084_cov_2.351798, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001709 DAORHD010000000
VERSION     DAORHD010001709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_201999_length_1084_cov_2.351798"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PKO06_24650"
     CDS             complement(<1..446)
                     /locus_tag="PKO06_24650"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72922.1"
                     /translation="MMTTTTRNNRSRRTTRTRFERVGFTLIEMLVSIAIGLMAMFMIY
                     NFLGSTRMHYMYGTVNLQNLQDARLAINYLRRDFSSSCFFVSPSDAFKTVQLARMSGF
                     KSGTQWSGGNSHLIEITNKKLSFHRFDFGIDQAMGTPTVKLVEYEF"
     gene            complement(449..973)
                     /locus_tag="PKO06_24655"
     CDS             complement(449..973)
                     /locus_tag="PKO06_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV72923.1"
                     /translation="MRRRAGFTFAEIMVAILVAAVLAVPLFYLVTSTRTDTSKAINYL
                     RAMELANEAIEWANVIPFHKIKELEGTGGSLVEDLGGNFVPVKLAVVNNGMWAGLAPQ
                     IQYSDQYPTAFFHREIIVGSITQSVPWRDAMAKVTVTVSWNEGKKPDILDSTDRMRKI
                     TLYALIFNDRKLDY"
BASE COUNT          235 a          313 c          282 g          254 t
ORIGIN      
        1 tcgaactcgt attcgaccag tttgaccgtc ggcgtgccca tcgcttgatc aatgccgaaa
       61 tcgaatcgat ggaacgagag tttcttgttc gtgatctcga tcaggtgtga gttgcctccc
      121 gaccactgtg tgccgctttt gaagccgctc atgcgggcca gctgaaccgt cttgaaggca
      181 tcagacggag aaacaaaaaa gcagctcgag gaaaaatctc gtcgcagata attgatcgcc
      241 agacgagcgt cctgcagatt ctgcaggttc accgtgccgt acatgtagtg catccgggtt
      301 gagcccagaa agttgtagat catgaacatc gccatcaaac cgatggcaat gctgaccagc
      361 atttcgatca gggtaaaccc cactcgctcg aagcgcgtgc gagttgtgcg gcggctccgg
      421 ttgttgcgtg tcgtggtggt catcatggtc aatagtccag tttgcgatcg ttaaagatca
      481 gcgcatagag agtaatcttg cgcattcgat ctgtcgaatc gagaatatcc ggtttcttgc
      541 cctcgttcca ggagaccgtc accgtcacct tggccatggc atcacgccag ggaacgctct
      601 gagtgatcga ccccacgatg atttcgcgat gaaaaaacgc ggtcggatac tggtcggaat
      661 actggatctg aggagccaag ccggcccaca tcccattatt gacgaccgcc agtttcacgg
      721 gcacgaagtt tccccccagg tcttcgacca gcgagccgcc tgtcccttcc agttccttga
      781 ttttgtggaa ggggatgacg ttcgcccatt cgatcgcctc attcgccagt tccatcgctc
      841 gcagatagtt gatcgccttc gacgtatccg tccgcgtcga agtgaccaga tagaacagcg
      901 gtaccgccag cacggcggca accagaatcg caaccatgat ttcggcaaac gtaaatccag
      961 ctcttcgtct cactttttct cttccttggc tggtactatc gcatcggctg cagaggccag
     1021 ctgcgccagc tccttgagcc cgcggcgcct ctcgtctccc tgcttgaact gttgcaccag
     1081 atca
//
LOCUS       DAORHD010001710         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_202516_length_1082_cov_3.075949, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001710 DAORHD010000000
VERSION     DAORHD010001710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202516_length_1082_cov_3.075949"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /gene="amrS"
                     /locus_tag="PKO06_24660"
     CDS             complement(<1..948)
                     /gene="amrS"
                     /locus_tag="PKO06_24660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV72924.1"
                     /translation="MNRRQFLQSCTGLCALASSGWVSFPALGAEIISEPHDAEYWELL
                     PDGRVHCLLCPNGCVRGDRERSLCRAREPRNGALKSLVWGNPCVLALDPIEKCPLFHY
                     QLPGPAFSIATAGCNLACSYCQNWQFSQKPPEETRNFSMRPSDVVAKALEYKASAIAF
                     FYTEPTIYIEYMKDVARAARSKSLTTVMVTAGSINEKPLADLLELIDAFTIGLKGFTD
                     EYYRTVIKGSLQPVLAAIKQVKASGRHLELVNLLVPTLNDAEGDVSKLVDWVGDHLGT
                     EVPLHFTRFNPQFQLKSLPPTPVPVLERARRLAQKKGLRF"
BASE COUNT          203 a          308 c          336 g          235 t
ORIGIN      
        1 gaaacgcagt cccttcttct gagccagacg ccgtgcgcgc tccagcaccg gaaccggggt
       61 tgggggcaga gacttgagct gaaactgtgg attgaagcgc gtaaagtgca ggggcacctc
      121 cgtcccgaga tgatctccca cccagtcgac gagcttgctc acgtcgcctt cggcgtcatt
      181 cagggtgggc acgagcagat tgaccagctc gagatggcga cccgaagcct tgacctgttt
      241 gatggcagcc agaacgggct gcagcgaccc tttgatgacg gtgcggtagt actcgtccgt
      301 gaaccccttc agaccgatgg taaacgcatc gatgagttcg agcaggtctg ccagcggctt
      361 ttcgttgatc gaaccggccg tcaccatcac cgtggtgagc gacttgcttc gggccgcccg
      421 cgcgacgtct ttcatgtatt cgatgtagat cgtcggctcg gtgtagaaga aggcgatcgc
      481 tgacgccttg tattcgagcg ctttggcaac gacatccgag ggacgcatgg aaaagttgcg
      541 cgtttcttcc gggggcttct gactgaactg ccaattctgg cagtaactgc acgcgaggtt
      601 gcagccggcg gtagcgatcg agaaggccgg accgggaagc tgataatgaa acaacggaca
      661 cttctcgatt gggtcgagag cgagcacgca ggggtttccc cacacaagcg atttgagcgc
      721 gccgttccga ggttctcgtg cccgacagag actgcgttct cgatcacctc ttacacaccc
      781 gttcggacag agaagacagt gtacgcgccc gtctggaagc agttcccagt attcggcgtc
      841 atgcggctct gaaatgatct ccgcgcccag ggccgggaag gagacccatc ctgaagaggc
      901 cagggcgcag agccccgtgc aggactgcag aaactgtcgc cggttcattg gtatctcccc
      961 tttctctcct gcttcctttt cgatcctcgg ggtggctctg tggttacggt tttttttcgg
     1021 tcgaatctgg tttgagcgtg cccgtcggcg gggttgccgg cttgacacct ggtttggcgg
     1081 tc
//
LOCUS       DAORHD010001711         1082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_202530_length_1082_cov_2.933788, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001711 DAORHD010000000
VERSION     DAORHD010001711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202530_length_1082_cov_2.933788"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1082)
                     /locus_tag="PKO06_24665"
     CDS             complement(<1..>1082)
                     /locus_tag="PKO06_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNV72925.1"
                     /translation="DRYNFTVNEAEPLEKEVAIDPEMLGKVFENLIEENRRKGLGAYY
                     TPREIVHYMCQESLINYLDTALNKEKALVPRKDIETFIHTGDQAAHYEAARISGTKSY
                     KPELPKPFETHARLIDDKLQDITVCDPAVGSGAFPVGMMTEIVRTRSAITPYFNDVHE
                     RTPYHFKRHAIQNCLYGVDIDPGAVEIAKLRLWLSLVVDEEDVKQVRPLPNLDYKIMS
                     GNSLFGVEKDLFNVEAFKQLELLKPRFFDETDDVRKKIIKGQIDRLIVELTNGEKQFD
                     FEIMFSEVFHKTQGFDAVIANPPYVRQESIKEFKPQLKQAFGGFYCGTADLYTYFYKR
                     GLEILKPAGHLCFIAPNKFMRAGYGE"
BASE COUNT          232 a          265 c          300 g          285 t
ORIGIN      
        1 tctccccgta gccggcgcgc atgaacttgt tgggtgcgat gaagcagagg tggcccgcgg
       61 gcttgagaat ctccaggccg cgtttgtaga agtaggtgta aagatcggca gtaccgcaat
      121 agaaaccacc gaaggcctgc ttcaattgag gcttgaactc cttgatggat tcctgacgca
      181 catagggtgg gttggcgatg actgcatcaa agccttgcgt cttatggaaa acttccgaga
      241 acatgatctc gaagtcaaac tgcttttcgc cattcgtcag ttcgacgatg agtctgtcga
      301 tctggccctt gatgatcttc ttcctgacgt cgtcggtctc atcaaagaat cgtggtttca
      361 gaagttcgag ttgtttaaac gcctcgacgt tgaagagatc tttctcgacg ccgaaaagcg
      421 agttgccgga catgatcttg taatccagat tcggcagagg tctgacctgc tttacatctt
      481 cttcgtccac cacaagcgac agccagaggc ggagcttggc gatctcgact gcgccggggt
      541 cgatgtccac accatagagg cagttctgga tagcatgacg cttgaaatgg tagggggtac
      601 gttcgtgcac gtcattgaag taaggtgtaa tggcggaacg ggtgcgcacg atttcggtca
      661 tcatgcccac cgggaaggcg cccgacccga cggcagggtc gcaaacagtg atgtcttgca
      721 acttgtcgtc aatcaatcga gcatgtgttt cgaaaggttt aggcaattcc ggtttatagc
      781 ttttcgtgcc tgatatacgc gcagcttcgt aatgggcggc ctgatcgcct gtatgaataa
      841 aggtttctat gtcctttctc gggaccagcg ccttttcttt gttcagtgcg gtgtcgagat
      901 aattgatcag actctcctgg cacatgtaat ggacaatttc gcgtggcgtg taataagcgc
      961 ccaacccttt ccggcggttt tcctcgatca ggttttcaaa aaccttgccc agcatttcgg
     1021 gatcaatggc gacttctttt tccaggggct cggcctcgtt gacggtgaag ttgtaccggt
     1081 cg
//
LOCUS       DAORHD010001712         1081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_202996_length_1081_cov_2.118908, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001712 DAORHD010000000
VERSION     DAORHD010001712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_202996_length_1081_cov_2.118908"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKO06_24670"
     CDS             <1..622
                     /locus_tag="PKO06_24670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV72926.1"
                     /translation="VGHFAEGLQYKFIKIVRGPIEIASDGQWAELEVPRGGVGELLVA
                     GEHVCRAYYNNPEAFKTSKIRDHNDIVWHRTGDLGRLDEKGNVWIVGRIHNVIVRDGT
                     YLVPVQAEVVLKRQPYTRLAAYLGVPDLKLGEKTVCVIAPKDNADIKNTKLCAERETE
                     IRRVMTKNEIPVDQVIFHPDIPMDLRHRSKVEYGILREELKKSGLV"
     gene            complement(746..>1081)
                     /locus_tag="PKO06_24675"
     CDS             complement(746..>1081)
                     /locus_tag="PKO06_24675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72927.1"
                     /translation="RGKYVSELKFSDFTVDDNIQLLPDTVTLIHKDQQGRIIPHETVS
                     HDLDETPKSRDRLPSYRKVYDAETLPDDITEEIVCEFLIKGTRERVAFTLPIKKVARY
                     SGYDVLMGV"
BASE COUNT          251 a          308 c          302 g          220 t
ORIGIN      
        1 cgtcggtcac ttcgccgagg gtctgcagta caagttcatc aaaatcgtac gaggccccat
       61 cgaaatcgcg tcagacgggc agtgggccga gctcgaagtg ccgcgcggag gcgtcggcga
      121 actgctggtc gccggcgaac acgtctgccg cgcgtattac aacaaccccg aggcgttcaa
      181 gacctcgaaa attcgtgacc acaacgacat cgtctggcat cgaaccggcg atctcgggcg
      241 cctcgacgag aaggggaacg tctggatcgt cggtcgcatt cacaacgtga tcgtgcgcga
      301 cggcacgtat ctcgtcccgg tgcaggccga agtcgtgctg aagcgccaac cctatacccg
      361 tctggccgcg tatctcggcg tgcctgattt gaagctcggc gaaaagaccg tctgcgtgat
      421 cgccccaaaa gacaacgccg acatcaagaa tacgaaactc tgcgccgaac gcgaaaccga
      481 gattcgccgg gtcatgacga agaacgaaat cccggtagat caggtgatct tccaccccga
      541 catccccatg gatctccgcc atcgctcgaa ggtcgaatac ggcattctcc gcgaggaatt
      601 gaaaaagtcc gggttggtgt gaagtcagac ggagatccat actgacttga acgagtaaag
      661 aagcgacagc gcaagggtct gtcgtgcaac cagacctgct gaggccctcg aatctcatct
      721 ggagtttcgg gggcttgttt cgcatctaaa cacccatcag gacatcgtaa ccagagtaac
      781 gcgccacttt cttgatggga agcgtgaaag cgacccgttc ccttgttccc ttgatcagga
      841 actcgcagac aatctcttcg gtgatgtcat cgggtaaagt ctccgcatca tacacttttc
      901 tgtaggaggg gaggcgatca cggctctttg gcgtttcgtc cagatcatgg ctcaccgttt
      961 catgaggaat gatccggccc tgctgatcct tgtgaatcag ggtaaccgtg tccgggagca
     1021 actggatgtt gtcatccacg gtgaaatccg agaacttgag ctctgaaacg tactttccgc
     1081 g
//
LOCUS       DAORHD010001713         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_203236_length_1080_cov_2.844878, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001713 DAORHD010000000
VERSION     DAORHD010001713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203236_length_1080_cov_2.844878"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PKO06_24680"
     CDS             complement(<1..1029)
                     /locus_tag="PKO06_24680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV72928.1"
                     /translation="MTRPASETSVVQAFDCDLLIGSLTTIATFPRESPVATGTVDVSF
                     SFRDTQRPHTVTLIGTDGSEPRNCSAAASLTLPAGTYRLLIEDSDGCRGVFDQIASRL
                     NANPVFIGTHLPEDQDFRICPLNFWDTGFPTIYGLNLHGQLFSALSRQDFLSSTFVHE
                     DRYPRAWPLITLLVLLPLIVLADTVFLRHGAIRGGLLTLVLAGAWIFVSISLFLNQQL
                     LPIALPLIVFFSFGTSRGYFAYMQARRREQQTRGSFSRFVSARMVDEILKNPNALKPG
                     GEKCELSVMFTDLAGFTSISEMLSPEELTRLMNEYLGEMTKILIQYGGTLDKYIGDAV
                     MAFWNAPMN"
BASE COUNT          283 a          273 c          316 g          208 t
ORIGIN      
        1 attcattggg gcattccaaa acgccatgac ggcatcaccg atgtatttat cgagagttcc
       61 gccatactgg atgaggatct tggtcatctc accgagatat tcattcatca accgcgtcag
      121 ttcttctggg gaaagcattt ccgaaatact ggtgaagccg gcaagatcgg taaacatcac
      181 cgacagttcg cacttttcgc cacccggttt cagcgcattc gggtttttca gaatctcatc
      241 aaccatgcgt gcggacacga aacggctgaa gctgccacgg gtctgttgct cgcggcgtct
      301 ggcctgcata taggcaaagt aaccgcgtga ggtgccgaag ctgaaaaaca cgatcagagg
      361 cagggcaatc ggcaacaact gttgattcag aaacagcgag atactgacga agatccaggc
      421 gccggcaaga acgagcgtga gcaggcctcc tcgaatcgct ccgtggcgca gaaaaacggt
      481 gtcggcaagc acgatcagag gcagaagcac caacagcgtg atcagcggcc aggcacgcgg
      541 atagcggtcc tcatgtacga acgtcgagga gagaaagtcc tggcgactca aagccgaaaa
      601 tagctgccca tgcaaattca aaccataaat cgtgggaaaa cccgtatccc agaaattcag
      661 ggggcagatg cgaaagtcct gatcttccgg taagtgggtt ccgatgaata cagggtttgc
      721 attcaaacga gaggcaatct ggtcaaagac tccccgacat ccgtcggaat cttcaatcag
      781 caggcgatac gtgccagctg gaagcgtcag agaagcggcg gcagagcagt ttctcggctc
      841 tgatccatcg gtgccgatca gagtgacggt gtgcgggcgc tgtgtgtcgc gaaagctgaa
      901 tgagacgtcg actgttcccg tcgcgacagg cgactcccgg ggaaaggtgg cgatggtggt
      961 gaggctgccg atgagaagat cgcagtcgaa ggcctgaacg acagacgttt ccgacgctgg
     1021 tcgagtcaac aggtatctgg cgccgagaga agaatacaac ccgtgaatgg tgatctgttt
//
LOCUS       DAORHD010001714         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_204066_length_1078_cov_2.088954, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001714 DAORHD010000000
VERSION     DAORHD010001714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204066_length_1078_cov_2.088954"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKO06_24685"
     CDS             <1..1041
                     /locus_tag="PKO06_24685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72929.1"
                     /translation="QVESHLARDRGLEQLGRFLIQLLDVNDLVREMSQLLETSISRKV
                     ALDLCFADPIPPIRGDATQIRQVVMNLIINASDAIGERSGHVTVRTGVTKIDNEIWDD
                     NYFLERIADGLYVFFEVADDGCGMSEVTMRRIFDPFFTTKFTGRGLGLAATLGIIRSH
                     KGAISVKSVVDQGTTFRVLIPASEGVATKPVSLTATARPAPRGISEPVPQQSEPLLSS
                     KGLILLADDEAAIRSLAVKMVKRCGYDLILAADGVEACRLFRERHNEIGLIVLDLTMP
                     RMSGEEAFRALRELDSEIPILISSGFDENDTMTRFVGAEHVAYLQKPFLFQDFSARLD
                     EMIRGKRPTEPR"
BASE COUNT          204 a          322 c          328 g          224 t
ORIGIN      
        1 caggtcgaga gccaccttgc gcgagatcga ggtctcgagc aactgggacg tttcctgatt
       61 cagttgctgg atgtcaacga tctggttcgc gaaatgtccc agttgctcga gacctcgatc
      121 tcgcgcaagg tggctctcga cctgtgcttc gccgacccga taccgccgat tcgcggcgac
      181 gcgacgcaga ttcgtcaggt cgtgatgaat ctgatcatca acgcctctga cgccatcggc
      241 gagcgctcgg ggcacgtcac ggtcaggacc ggcgtgacca agatcgacaa cgagatctgg
      301 gacgacaact acttcctgga gcgaattgcc gacggcctct acgtgttttt cgaagtggcc
      361 gacgatggct gcgggatgtc cgaagtgacg atgcgacgca tcttcgatcc gttcttcacc
      421 accaagttta ccggccgggg gttgggactg gcagccactc tgggcattat acgcagccat
      481 aagggggcca tctcggtcaa gagcgttgtc gatcagggaa cgacgtttcg cgtactgatt
      541 ccggcttctg aaggggtggc gaccaagccg gtttcgctca cggcgaccgc gcgcccggcc
      601 ccgaggggta tctctgagcc ggtgccgcaa cagtctgagc cattgctctc atccaaaggc
      661 ctgattctgc tggcagacga cgaagcggcg attcgctccc tggcggtcaa gatggtgaaa
      721 cgctgtggct atgatctgat tctcgccgcc gacggagtcg aagcgtgccg tctgtttcgt
      781 gaacgccaca acgagatcgg gctgatcgtg ctcgatctga ccatgccgcg catgagtggg
      841 gaagaggcat ttcgggccct gcgcgagctc gattcagaga tccccatctt gatctcgagc
      901 ggcttcgacg aaaacgatac catgacccgt tttgtcgggg ccgaacatgt cgcctacctg
      961 cagaaaccgt tcctgttcca ggacttttcc gcccgcctcg acgagatgat tcgcggaaaa
     1021 cgtccgaccg aaccgcgctg atggcgtgat ctgtgctccc cggccattgt ccgccggg
//
LOCUS       DAORHD010001715         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_204511_length_1077_cov_1.563601, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001715 DAORHD010000000
VERSION     DAORHD010001715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204511_length_1077_cov_1.563601"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PKO06_24690"
     CDS             <1..845
                     /locus_tag="PKO06_24690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72930.1"
                     /translation="ESLKAASGEFTRVSWARLFVFHPWFPVFDLGVNEYGSFGIQDSV
                     VGLSPDPELKTKPEFTWQPVETLPTRHSKAVVPGALLILLYLALGALTVVGPYLGLEW
                     LFQRDRALAERALLDTELAILDNQLESAENMLNSWNPGSISWYGGYENTIYDTRKREL
                     ATWVKIRRATASAEDVSGLYSGSPADLIKAEHLVRTGNASDAVTLYGGLMTYEGEHWR
                     LFPSLMDSIRWLLKKGDVSSATALAHRCLAHLPEWKRQDPTTEEFRLFLNFCKENPPP
                     AEAH"
BASE COUNT          241 a          321 c          282 g          233 t
ORIGIN      
        1 cagagtcact caaggccgca tccggggagt ttacccgggt ctcctgggcc cggttgttcg
       61 tgtttcatcc gtggtttcca gttttcgatc tcggcgtcaa tgagtatggc tccttcggca
      121 ttcaggattc cgtcgttggt ctgagccctg accccgagct gaaaacaaaa cccgagttca
      181 cctggcaacc cgttgaaaca ctgccgacgc gtcattcgaa agcagtcgtt cccggagcgt
      241 tgctcattct gctctatctt gcactcggtg cgctcacagt cgttggtccg tatctcggcc
      301 tggaatggct gtttcaacga gatcgcgctc tggcggaacg tgctctgctc gataccgaac
      361 tcgccattct cgataaccag cttgaatccg ccgaaaacat gctcaattcg tggaatcccg
      421 gctcaatcag ctggtacgga ggctacgaaa acacgatcta cgacacgaga aaacgcgaac
      481 tcgccacctg ggtgaaaatc cgacgtgcca cggcttcagc tgaggatgtt tcgggtctct
      541 actcaggatc cccggccgac ctgatcaaag ccgaacatct ggttcgcacc ggcaacgcct
      601 ctgatgctgt gactctctac ggcgggctca tgacctatga aggtgaacat tggcgtctgt
      661 ttcccagcct catggactcg attcgttggc tgttgaagaa aggtgatgta tcttcagcaa
      721 ccgcgcttgc ccatcggtgc ctcgcccacc tgcccgaatg gaaacgccag gatccgacga
      781 cggaagaatt ccgactcttt ctgaacttct gcaaggaaaa cccgccaccc gctgaagcgc
      841 attgatcact cgaatcgcca acccgaacag gcggcagcgg ggagaggtat cagagacatc
      901 gcactttatc gagcgccgag aacctcgccc agaaagtggc gcaacagacg gcgggcccag
      961 aatacccagg gaaagatgaa gatctgcagc ggtaccagca gtgtcagggc aaacaacatt
     1021 ttctgttcca gaagcagcgt ctgacactgc tggtaccgct gcagatcttc atctttc
//
LOCUS       DAORHD010001716         1076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_204608_length_1076_cov_3.166503, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001716 DAORHD010000000
VERSION     DAORHD010001716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204608_length_1076_cov_3.166503"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PKO06_24695"
     CDS             complement(<1..1020)
                     /locus_tag="PKO06_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72931.1"
                     /translation="MRWRRLLSFAVCFICLSVWSALAARSWFLFDQPPREGSEPLTAL
                     ATLIEGDDTWVGYAGYGLVRYNYFGQITVSLTVKDGLPGPTVTDLAKRDKDLWIATSD
                     GLAVRNEAGTLRVFTTKDGLPDNGITCLTVRDDTVYAGTMKGLVRFRGDSFDILDENH
                     GLPAAHITALGVCDNGIMIGTPKGWGVLRGSQIEAHTPQIDGLPFEWITAVAHYRYRK
                     LAMMDANANTADEYYVLGTAGGGLIYYRGGQYTVVGPGDDGPGAAWITCLTYVPEEQK
                     LWVGTQEGLAICTVADGTWERFTPQNSGLASPMVKDISIKVIDETVRDYEDQILKGYG
                     LACPCA"
BASE COUNT          206 a          348 c          323 g          199 t
ORIGIN      
        1 cgcgcatggg caagccagcc cgtatccttt cagaatctga tcctcataat cgcgcaccgt
       61 ttcgtcgatg actttgatcg aaatgtcctt gaccatcggg ctggccaacc ccgagttctg
      121 cggggtgaac cgctcccagg ttccgtcggc gaccgtgcag atggccaggc cttcctgcgt
      181 gccgacccag agcttctgtt cctcgggcac gtaggtgagg caggtgatcc aggcggcgcc
      241 ggggccgtcg tcgcccgggc cgacaaccgt gtactggccg ccgcgatagt agatcagccc
      301 gccaccagcg gttccgagca cgtaatactc gtccgcggtg ttggcgttcg catccatcat
      361 ggccagtttg cgatagcgat agtgagccac ggcggtgatc cactcgaacg gcaacccgtc
      421 gatctgcggg gtgtgcgcct cgatctgact gccgcgaaga accccccagc ctttcggcgt
      481 accgatcatg atgccgttat cacagacacc gagtgcggta atgtgcgcgg cgggcagtcc
      541 gtgattctcg tcgagaatgt caaaactgtc gccccgaaat cgtaccagtc ccttcatggt
      601 gcccgcatag acggtgtcat cgcgcacggt cagacaggta atgccgttgt ccggcagccc
      661 gtctttcgtg gtgaacaccc gcagagtgcc ggcttcgtta cgcactgcca gtccatctga
      721 cgtagcgatc cacaggtcct tgtcgcgctt cgccagatcg gtcacggtcg ggcccggcag
      781 cccgtctttc acggtcagcg aaaccgtgat ctgaccaaag tagttgtaac gtaccagccc
      841 gtacccggca tacccgaccc aggtgtcgtc cccttcaatc aacgttgcca gcgccgtcag
      901 cggctcgctt ccctctcgcg ggggctggtc gaagagaaac cacgaccgag cggcgagggc
      961 cgaccagaca gaaagacaga tgaagcagac tgcgaaagac agcaggcgcc gccaccgcat
     1021 tggggggaac cggcgaaaga gggggggcga ttgatccggg ccttcaccac acatgc
//
LOCUS       DAORHD010001717         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_204927_length_1075_cov_3.546078, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001717 DAORHD010000000
VERSION     DAORHD010001717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204927_length_1075_cov_3.546078"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKO06_24700"
     CDS             <1..901
                     /locus_tag="PKO06_24700"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HNV72932.1"
                     /translation="LFAVFSSPIAHLGKPRRLDFSTVTNATATPLLSTPSLKVAVTKF
                     FLLAGNDTFIRALFSEFERRQTTGTTHSLAFGISSERLYEQLCQNKVDLALVGAGVTA
                     LLPASSEYELLATPVANGTTTSAVCLVVPASSTVRSIADLRDKTFIFTDSLSYSGYAF
                     PRKLVSDLGYLPESFFRHTLISPSHENTVYLLKKGFGDGAVIDRPVLEWAMRAHPELR
                     AHFRIVTEWLGMPMPQMLVSKSLPVSYRTALREFFLTLHETPSGRIILGNTFIERFVA
                     ADAQVYENLRSSMIQFGSLKISP"
BASE COUNT          205 a          335 c          270 g          265 t
ORIGIN      
        1 gttgttcgcc gtcttttcgt cgcccatcgc ccatctgggc aaaccgcgcc gccttgattt
       61 ttcgactgtc accaacgcaa cagccacacc cctgctctcg accccgtcac tcaaggtggc
      121 tgtcaccaag ttctttctgc tcgcaggaaa cgatacgttt atccgagcac ttttcagcga
      181 gttcgagcgt cgacagacga cgggaacgac gcattcgctc gcgttcggca tttcgtctga
      241 acgtctgtat gagcagctct gtcaaaacaa ggtcgatctc gctctcgtcg gagccggtgt
      301 gaccgcgctt ctgccggctt cgagcgagta tgaactgctg gccaccccgg tcgcgaatgg
      361 caccacgacg tctgccgtct gcctggtcgt gcctgcgtcg tcaacggtgc gcagcatagc
      421 cgatctgcgc gataaaacat tcatcttcac agattcgcta tcctattcag gatacgcatt
      481 tccgcgaaaa ctggtgagtg acctcggtta tctgcccgag tcgttcttcc gacacacgtt
      541 gatctcgccc agtcacgaga acaccgtgta tcttctgaaa aagggattcg gcgacggagc
      601 agtgattgat cgcccggttc tcgagtgggc catgcgagcc catccggagt tgcgggccca
      661 ctttcgtatc gttaccgaat ggctcgggat gccgatgccg cagatgctcg tctcgaagtc
      721 tctgcccgta tcctatcgca ccgctcttcg cgagtttttt ctcaccctgc atgaaacgcc
      781 ctcgggtcgc atcattctcg ggaacacgtt catcgagcgt tttgtcgctg ccgacgctca
      841 ggtctatgag aacctgcgat cgtcgatgat tcagttcgga tccctgaaga tttcgccatg
      901 agtatcagac gaaccgtcac cctctcgttt ctctttctgt cagcactgct gatcatcggc
      961 gttaccggtg tcctgttcct gcagacaaga aactttctgc tcagccagca acttcgagga
     1021 accgaagccc tctctcagct cgcggccaac agaattgagg aactggtact gctct
//
LOCUS       DAORHD010001718         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_205582_length_1073_cov_3.361493, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001718 DAORHD010000000
VERSION     DAORHD010001718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205582_length_1073_cov_3.361493"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PKO06_24705"
     CDS             <1..217
                     /locus_tag="PKO06_24705"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002685498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase, FolE2/MptA family"
                     /protein_id="HNV72933.1"
                     /translation="DEKELTERAYARPRFAEDLVREVALRLDAQPEITWFTVETENLE
                     SIHAHSAYAFLKKDKRRSRVGRSTGAR"
     gene            complement(230..>1073)
                     /locus_tag="PKO06_24710"
     CDS             complement(230..>1073)
                     /locus_tag="PKO06_24710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72934.1"
                     /translation="KRAPTLGGPARQQRLDGFSLPPDTRIPDGLKPLLNDRRYPRQGV
                     STTGYTGSLQVPSPGVLEPGKSGVGLHMTAFNLYGINDQRYDDDAYFDTNVSLLYGAF
                     DGFEIGIDKTFSNQDRFDVPEPAFVNCKYQVPGNVTLGGSFSTDSQAAYHSAWISAGV
                     PVLWVGAGVNFGAGDFQFSYVGRDRLKRAKFGGYNYDYNKARGYADPAFFMIGGAVPL
                     NRYLNFAYDFNGDKFSLGFRFNYQNTVYFDAAYLADGDYERLPGAIAHKRLRNFVFGT
                     SIRY"
BASE COUNT          238 a          310 c          319 g          206 t
ORIGIN      
        1 tgacgagaag gaactgacgg agcgcgccta cgcacgcccc cgctttgccg aagatctggt
       61 gcgcgaggta gcgcttcgtc tcgatgcgca gcccgagatc acctggttta ccgtcgaaac
      121 cgaaaacctc gagagtattc atgcgcacag tgcctatgcc tttctgaaaa aggacaagcg
      181 ccggtctcga gtgggacgat cgaccggcgc tcgttgagac gaaacgactt cagtaccgaa
      241 tgctggttcc gaaaacgaag ttgcgaagcc gcttgtgggc gatggctccc ggaagtcgtt
      301 cgtaatctcc gtcggccaga tacgcggcgt cgaagtagac cgtgttctga tagttgaacc
      361 gaaaacccag actgaacttg tcgccattga aatcgtaggc gaagttcaga taccggttca
      421 gcggcaccgc gccgccgatc atgaagaacg cggggtcggc atacccgcgg gctttgttgt
      481 aatcgtagtt gtagccaccg aacttggcgc gcttcaggcg gtcgcgaccg acatacgaaa
      541 actgaaaatc tccggcgccg aagttcaccc cggcaccgac ccacaagacg ggcacgccgg
      601 cgctgatcca ggcgctgtga taggccgcct gtgaatccgt gctgaaactg cctcccagcg
      661 tgacattgcc ggggacctga tacttgcagt tgacgaacgc cggctcggga acatcgaacc
      721 gatcctggtt gctgaaggtc ttgtcgatgc cgatctcgaa cccgtcaaaa gcgccataca
      781 gcagactgac gttggtatcg aaatacgcat catcgtcata ccgctgatca ttgattccgt
      841 acaggttgaa ggcggtcatg tgaagcccaa caccgctctt tccgggttcg agaaccccag
      901 gtgaaggaac ctggagggaa ccggtgtagc cggtcgtcga aactccctgc cgcggatatc
      961 gacgatcgtt gagcagcggc ttcaacccat cggggatccg ggtgtcgggc ggcagagaaa
     1021 agccgtcaag tctctgttgg cgtgccggac cacccagcgt cggcgcgcgt ttt
//
LOCUS       DAORHD010001719         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_206720_length_1070_cov_2.382266, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001719 DAORHD010000000
VERSION     DAORHD010001719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_206720_length_1070_cov_2.382266"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..>1070
                     /locus_tag="PKO06_24715"
     CDS             261..>1070
                     /locus_tag="PKO06_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72935.1"
                     /translation="MSEKRQAQQRVSSGGSGPISAHVKPSRHIFQAIPWEHVSPVPSN
                     EGFLPSLKRLALFLKDIAKADAVGIRLQQNSDFPFYFTRGFSRDFVRAENRLCRLDEQ
                     GNFVCDRLGVPLLECMCGFVLRHRPRRLVRGFSRRGSFLSTSRQEFHEILREVGETVP
                     LHGRCHAEGFETIGIMPLRRGKRTFGVLQFNWRRTAAITWEKFLFLERVAENAARSLF
                     LQERHEHLQERLHAHHCAEESQGEVVLRLTPDGTMAYVNPAFCEAVGVARSA"
BASE COUNT          215 a          283 c          342 g          230 t
ORIGIN      
        1 gagaacgcgt cagcaccggt cgccgtcgat gtcccgggag ttgagaacca ctgaacaggt
       61 gtgagaccag cctgacgggc gcctgttgga tgtgctgatg cttgagatgc ttgtggtcta
      121 gagcgattgg ctggctcaca cgtttcgcac gccaggagaa aaagggagga gaacgaaatg
      181 accctgatga gtcggggtcg cgcttgcggg atgggtggag agcaggtaaa atcaaacgta
      241 ctctgagaga gtgagcaggt atgtcggaga aacgtcaggc ccaacagcgc gtctcatccg
      301 gcggttctgg cccgatcagc gcacacgtca aaccatcgag acacatcttt caagccatcc
      361 cctgggaaca cgtttccccg gtaccgtcga atgaaggttt tctgccgagc ctgaaacggc
      421 tggctttgtt cttgaaagac attgcaaagg cagatgcagt cggcattcgg ctgcaacaga
      481 actcggactt tcccttttac ttcacgcggg ggttctcgcg cgatttcgta cgcgctgaaa
      541 accgtctctg ccgactcgat gaacagggca acttcgtctg cgatcgtctt ggagtgccgc
      601 ttctggagtg catgtgcggg tttgtcctgc gtcaccggcc acggcgactg gtgcgcggct
      661 tttcgcgtcg cggcagtttt ctgtcgacca gccggcagga gtttcacgag attctgcggg
      721 aggtcgggga aacggtgccg ttgcacgggc gctgtcatgc ggaaggcttc gaaaccatcg
      781 gcatcatgcc gttgcgtcgt ggcaagcgaa ctttcggggt gttgcagttc aactggcgcc
      841 gcacagctgc catcacctgg gaaaaattcc tgtttcttga acgggtggct gagaacgccg
      901 cacgatcgct gtttctgcag gaacgacatg agcatctgca ggaacgtctg catgcgcacc
      961 attgcgccga agaatctcag ggcgaggttg tgctgcgact gactcccgat ggcacgatgg
     1021 cgtatgtgaa ccccgcgttc tgcgaagcgg ttggcgtggc tagatcggcg
//
LOCUS       DAORHD010001720         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_207925_length_1066_cov_4.498516, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001720 DAORHD010000000
VERSION     DAORHD010001720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207925_length_1066_cov_4.498516"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKO06_24720"
     CDS             <1..203
                     /locus_tag="PKO06_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV72936.1"
                     /translation="LKLTRGRTCLIIAHRLSTIRHCDRILVLHQGRLVESGTHDELLA
                     RKGTYFKLYELQFKDQNGNGHG"
     gene            200..853
                     /locus_tag="PKO06_24725"
     CDS             200..853
                     /locus_tag="PKO06_24725"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HNV72937.1"
                     /translation="MTEIDQASGMTCSDLKACHHCFSIQRLPDQVPPGHEVVCCGCGA
                     PFLTRGQVNQAWEKTSAFALTALILLLPSLFLPIMELETFGHRHASGIIAGVTSLFRE
                     GHFVLGGLILVCSVLFPLAKLSLLLFLSHSRDRLSTGGQVHLLHLLETAGRWGMLDVL
                     LIAILVAAVKLGNMLHITPGPAALLFTLAVCFSLLAGSAVNPDLVWHSTHRKETDDV"
     gene            846..>1066
                     /locus_tag="PKO06_24730"
     CDS             846..>1066
                     /locus_tag="PKO06_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72938.1"
                     /translation="MSETPAPFGPSPSPVPVASTRPVSSGSWIRWLPVPALLLVLGLL
                     IHSWWSRGPAVQIEFRQGYGLKSGDVIRFR"
BASE COUNT          167 a          341 c          299 g          259 t
ORIGIN      
        1 ttctgaaact gactcgcggt cgcacctgcc tgatcatcgc gcaccgactc tcgaccattc
       61 gccactgcga ccgtattctg gtgcttcacc agggccgtct ggtagaatca ggcactcatg
      121 acgagctcct tgcgcgcaaa gggacgtatt tcaagttgta cgagctgcaa ttcaaagatc
      181 agaatggaaa cggacacgga tgacggagat cgatcaggcc agcggtatga cctgcagcga
      241 cctgaaagcc tgtcatcact gtttttccat tcaacgactt cccgatcagg tgccccctgg
      301 tcacgaggtc gtctgttgcg gctgcggcgc tccgtttctg acccgcgggc aggtcaacca
      361 ggcctgggag aaaacctcgg cgttcgctct gacagcactg attcttctgc tgcccagtct
      421 gtttctgcca atcatggaac tcgaaacctt cggtcatcgt catgccagcg gcatcattgc
      481 cggggtcacg tcgttgttcc gcgagggtca cttcgtgctt ggcgggttga tacttgtctg
      541 ctccgttctc tttccgctgg ctaagctttc actgctgttg tttctcagcc actcccgcga
      601 tcgtctctcg accggtggtc aggttcatct gctgcatctg ctcgaaaccg ccggacgttg
      661 gggcatgctc gacgtgctgc tgatcgcgat tctcgtggct gccgtgaaac tcggcaacat
      721 gctgcacatt accccggggc cggcggccct gctgtttacg ctcgccgtct gcttcagtct
      781 gctggccggg tcggcggtca accccgatct ggtctggcac tcgacccacc gaaaggaaac
      841 cgacgatgtc tgaaactccg gccccctttg gtccgtcacc gtcgcccgtt cccgttgcct
      901 ctacccgtcc ggtctcttcc ggttcgtgga tccggtggct gcccgtgccg gccctgctgc
      961 tcgtcctggg cctgttgatt cactcctggt ggtcacgagg cccggcggtg cagatcgagt
     1021 ttcgtcaggg ctacggactc aagtcggggg acgtcatccg ctttcg
//
LOCUS       DAORHD010001721         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_207941_length_1066_cov_3.816024, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001721 DAORHD010000000
VERSION     DAORHD010001721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207941_length_1066_cov_3.816024"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1066
                     /locus_tag="PKO06_24735"
     CDS             <1..>1066
                     /locus_tag="PKO06_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72939.1"
                     /translation="ILSGLATRFVVDALGFAWTFLGVGVLLVAADLLLRRLGAFIQTH
                     ELPTPTVTPTGSAVAGDSWSRLPMATRHFSLLLIGFSFLAIFGRMLMDFLYNSQMAAA
                     FPSASELAGFLGLFWAAIDVISLLLQTLAGSWVFANLRLGTVMSVRAGAMTLIALIGA
                     WYPGPATAAAGWLALMTLTKAFVNPGFVIMLEPIPKHSRVMIRRYISIADASANMAAG
                     ALLLFLKAGGGRGSEAFLFLLVAVLFGIPLLAARLLDRWYAQTVEETLQQIDAEGDIE
                     IIHALRFVAPVERQSRMARLLQHQDPEVRFQTILETAELPPEPMADLLLTALVNEKEP
                     RNIAAMVRTILLRLGPMGEE"
BASE COUNT          164 a          293 c          343 g          266 t
ORIGIN      
        1 cgattctgag cggactggcg acccgatttg tggtcgatgc tctgggcttt gcctggactt
       61 ttctgggagt gggagtgctc ctggtcgcgg ctgatctgct gttgcgtcga ctgggggcgt
      121 ttatccagac tcatgaactg ccgacaccga ctgtgactcc gacaggaagt gccgtggccg
      181 gtgattcctg gtccagactg cccatggcga cacgccactt ttccctgctg ttgatcgggt
      241 tttcgtttct ggcgattttc ggtcgcatgc tgatggactt tctctacaac agccagatgg
      301 cggcggcctt tccttcggcc agtgaactcg cgggatttct tggtctgttc tgggcggcga
      361 tcgatgtgat ttccctgctc ctgcagacac tcgcgggcag ttgggtgttc gcgaatctcc
      421 gcctcggaac ggtcatgtcg gtgcgcgctg gagccatgac gctgattgct ctgattgggg
      481 cctggtatcc cggtccggcc acggccgcgg cggggtggct ggcactgatg acgctgacca
      541 aagcgttcgt caatccggga tttgtgatca tgttggagcc aattcccaag cactcgcgcg
      601 tgatgatacg tcggtacatc agcattgccg acgcttccgc gaatatggca gccggagccc
      661 tgctgctctt tctgaaagca ggtggcgggc gcggcagcga agcgtttctc tttctcctcg
      721 tggcggtgct gtttggcatt ccgttgttgg cggcgcgtct tctcgatcga tggtatgcgc
      781 agacggttga ggaaacgttg cagcagattg atgccgaggg ggacattgag atcattcacg
      841 ctctgcgttt cgtggctccc gtcgaaaggc agtcgcgcat ggcccgtctg cttcagcacc
      901 aggatcctga ggttcgcttt cagactattc tggagacggc tgagttgccc ccggagccca
      961 tggccgatct gctgttgacc gctctggtca acgagaagga accgcgcaac attgccgcga
     1021 tggtgcgcac catcttgctg cggctgggac cgatgggaga agagtt
//
LOCUS       DAORHD010001722         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_208330_length_1065_cov_3.221782, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001722 DAORHD010000000
VERSION     DAORHD010001722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208330_length_1065_cov_3.221782"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PKO06_24740"
     CDS             complement(<1..283)
                     /locus_tag="PKO06_24740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72940.1"
                     /translation="MPAETIRLRLHVLSPLHIGCGEVYEPTGFVIDERQMKLRAFDEF
                     AFVKSLPEDRRKRFGDICMTGTPLEVFKFVREHYLSDKVPPVRTVDVAAG"
     gene            complement(273..>1065)
                     /locus_tag="PKO06_24745"
     CDS             complement(273..>1065)
                     /locus_tag="PKO06_24745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72941.1"
                     /translation="AEGPAPENSAEGMKSRKQAKHNKWLVVNAENPVKPTTPHECKPE
                     TEIRDLIIAELPDADRRGLNRQSEKKLQISHPRLHCRVSRVTGATGAGALYKSDEVYY
                     LPGMRLAVFAGLADDLPAEGLKTAFERLGLQGYGRDASSGLGRFTVESCVPFELAKLG
                     AKGDDAWYVLGPCVPEKGVWTNTWFQPFVRFGRHGDVLARSRNPFKNPVIMADEGAVL
                     AGRRNRVEPYVGTALTGLSFEPETVGQGYALCLPIKVGEVATNAR"
BASE COUNT          225 a          333 c          286 g          221 t
ORIGIN      
        1 gaccagccgc cacatcgacg gtgcgtaccg gcggaacctt gtcagacagg taatgctcac
       61 ggacgaactt gaagacctca agcggagttc ccgtcatgca gatatcaccg aaccgcttgc
      121 ggcgatcttc aggcagagat ttcacgaacg cgaactcgtc aaacgctcgc agtttcatct
      181 gtcgttcgtc gatgacgaac ccggtcggtt cgtacacctc gccgcaaccg atatgtaacg
      241 gagacagcac gtgcaaacgc aaacgaatgg tttcagcggg cattggttgc cacctccccg
      301 accttgatcg gcagacagag cgcataaccc tgcccgaccg tttccggctc gaacgacaaa
      361 ccggtcagag cggtgccgac gtatggttcg acccggttcc ttctgcctgc cagaaccgct
      421 ccttcatcgg ccatgatgac cggattctta aacggattgc gtgagcgggc caggacatcc
      481 ccatgtctgc cgaaacgaac gaacggttga aaccaggtgt tggtccaaac gcctttctcg
      541 ggaacacacg gccccagcac ataccaggcg tcgtcgcctt tcgctcccag tttcgccagt
      601 tcgaacggaa cgcaactctc gaccgtgaat cgtccgagcc ctgaagaggc gtcacgccca
      661 tagccttgca atcccaaacg ttcgaaagcg gttttcaacc cctcggccgg cagatcatca
      721 gccagacccg cgaacacggc gagacgcatt ccgggcagat agtatacctc atcagacttg
      781 tacagagcgc cggcaccggt agcgccggtg acacgactga cccggcagtg caatcgcggg
      841 tgagagatct gcagtttctt ttcactctgt cggttgaggc cccgtcgatc agcgtcaggc
      901 aactcggcga tgatgagatc gcgaatttca gtttcaggct tgcactcatg aggcgtcgta
      961 ggtttcaccg ggttttcggc gttgactacg agccacttgt tatgcttcgc ctgtttgcgg
     1021 cttttcatgc cctcggcgct gttttccgga gcaggtcctt ccgcc
//
LOCUS       DAORHD010001723         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_208673_length_1064_cov_3.287413, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001723 DAORHD010000000
VERSION     DAORHD010001723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208673_length_1064_cov_3.287413"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PKO06_24750"
     CDS             complement(<1..784)
                     /locus_tag="PKO06_24750"
                     /inference="COORDINATES: protein motif:HMM:NF017893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HNV72942.1"
                     /translation="MTTNTSESQLSSFLKAPLVKIVVLGILVMVLMLPVVLIDGVISE
                     RRSSRDEVVREVTSSWGYSQRLVGPWIVVPYDYRWRETDKDGKEITRKETRYGWFLPE
                     NLEVSGTQTSEIRYRGIYKVPLYRVELKFNGRFPKPDLSSWVADPADVQWGNCHLVMR
                     VSDVRALVSRAFLQWNQSNLEFQPGTRESVSNFPGIHVPLTKEAFGESNEFSFSLAIL
                     GSDGLYFAPFGRDTKVVLKGNWPDPSFQGQWLPADRKITKEGY"
BASE COUNT          254 a          296 c          265 g          249 t
ORIGIN      
        1 gatagccttc cttggtgatc tttctgtcgg cgggcagcca ctgtccttga aagctcggat
       61 caggccagtt gcctttcaga acgactttgg tatcgcgacc gaacggcgcg aagtagagcc
      121 catcactccc cagaatcgcc agtgaaaacg aaaactcgtt cgattctccg aacgcttctt
      181 tcgtcagagg aacatggatt cccggaaaat tcgacacgga ttcacgcgtt ccaggctgaa
      241 actcgagatt cgactgattc cactgcagaa aggcgcgact gaccagagcc cggacatctg
      301 acactctcat caccagatga cagtttcccc attgaacatc cgccggatcg gcgacccagg
      361 acgagagatc aggcttggga aaccggccgt tgaacttgag ttcgacgcgg tacaggggaa
      421 ctttatagat cccccggtat ctgatttcag aggtctgcgt gccgctcact tcgagattct
      481 cgggaagaaa ccagccgtat cgggtttctt ttcgggtgat ctcttttccg tctttgtcgg
      541 tctcgcgcca gcgataatcg tagggaacca cgatccaggg tccgaccagt ctctgactgt
      601 atccccagct cgaggtcacc tctctcacga cttcatccct cgaacttcgg cgttcggata
      661 tcaccccatc gatcaggacg accggcaaca tcagcaccat gacgagaatt ccaaggacga
      721 caatcttgac cagaggcgcc ttgagaaacg aactcaactg ggactcagac gtgttggtgg
      781 tcatggaaga attccttgtt tgaactcggg agcgactcag agccgccccc tgaacccgac
      841 catcgtaaca ggtcacagtc aagagaagca atgaccaacg catcaaggtc gagtcatttg
      901 attctctggc ttgggttttt ctcttggcat cacgcaacga tttggtgtcg cggcagagag
      961 tgcaatattt gatctcgaag ctcaatatct gcgtttatca gtttttacca caaacctctg
     1021 ctctttctga agtcacgatg cagagaaggt tcagatgtga gcac
//
LOCUS       DAORHD010001724         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_210061_length_1060_cov_3.252736, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001724 DAORHD010000000
VERSION     DAORHD010001724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210061_length_1060_cov_3.252736"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKO06_24755"
     CDS             complement(<1..739)
                     /locus_tag="PKO06_24755"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009194547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNV72943.1"
                     /translation="MTNQARKIYYTLTDEAPALATVSLLPIIKAFASPAGVEVELRDI
                     SLAGRILAAFPESLTEKQRQNDDLAFLGQLATTPDANIIKLPNISASIPQLAAAIKEL
                     QEHGYKVPDYPMDPKTDQEKEIKNRYGKILGSAVNPVLREGNSDRRSAACVKEYAKKH
                     PHSMGKWEKTSKCHVAHMTENDFFGSETSHIAAQDGAVKIVHVGKDGKETILKDKIAL
                     QAGDVIDGASMSKKALRSFIEAQIKEAK"
     gene            complement(826..>1060)
                     /locus_tag="PKO06_24760"
     CDS             complement(826..>1060)
                     /locus_tag="PKO06_24760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72944.1"
                     /translation="PLEAHCSVSELLRLLARRRPVQTALARGSHRPAAAGHHPSQVSE
                     SATVVDSAGSARRRRTILKSAADRWPDEGLVFE"
BASE COUNT          199 a          310 c          292 g          259 t
ORIGIN      
        1 cttttgcttc cttgatctgc gcttcaatga acgaccgcag cgctttcttg ctcatgctcg
       61 cgccatcgat gacatcgccg gcctgcagcg caattttatc cttgagaatc gtttccttgc
      121 cgtccttgcc gacatgcacg atcttcaccg ctccgtcctg agcggcaatg tgcgaggttt
      181 cactgccgaa gaagtcgttt tcggtcatgt gagccacatg acacttcgag gttttttccc
      241 acttgcccat cgaatgcggg tgctttttcg cgtattcctt gacgcaggcg gccgaacgac
      301 ggtccgagtt gccttcgcgc agaaccggat tcaccgcgct tcccagaatc ttgccatagc
      361 gattcttgat ctctttttcc tggtcggttt ttgggtccat tgggtaatcg ggaaccttgt
      421 aaccgtgttc ctgcagttct ttgatcgccg cggccaactg cggaatcgat gcgctgatat
      481 tggggagctt gatgatattg gcgtcagggg tcgtggcgag ctgccccaaa aaagccagat
      541 catcgttctg gcgctgtttt tcggtcagag actcaggaaa cgccgcgaga atgcgaccag
      601 ccagcgaaat atcacgcagc tccacctcaa ctccagccgg actggcaaac gccttgatga
      661 tcggcagcaa cgatacggtc gccagcgcag gcgcttcgtc ggtgagtgtg taatagatct
      721 tacgagcctg gttcgtcatg atggtccatt cctttcagtt ctgtacgtat gttttttaag
      781 ggcaggccag aggatgactc gtgtcacccc ggaataccat agtttttact cgaataccaa
      841 tccttcatcg ggccagcgat cggcagccga tttcagaatc gttcgacgtc gccgcgcact
      901 cccggcagag tcgacaaccg tcgcgctttc tgaaacctga ctcggatggt gccccgcggc
      961 ggccggccga tgagagcccc gggcgagcgc ggtctgcact ggtcgacgcc gcgccagcag
     1021 gcgcagcagc tctgacacgg aacaatgcgc ttcgaggggg
//
LOCUS       DAORHD010001725         1060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_210108_length_1060_cov_2.777114, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001725 DAORHD010000000
VERSION     DAORHD010001725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210108_length_1060_cov_2.777114"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>1060
                     /locus_tag="PKO06_24765"
     CDS             176..>1060
                     /locus_tag="PKO06_24765"
                     /inference="COORDINATES: protein motif:HMM:NF018530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNV72945.1"
                     /translation="MHQLLLSSPYLLLFTVIGAGYVLGRINLGGLHLNVAAVLFVGLA
                     FGAWDKRFQIPEQVQMLGLILYIYAIGLQAGPIFFTVFRERGLRFNLLAIGTLAFSFL
                     VTLVVGRVIGVPSDLLAGLFTGGLTNTPALAAATDALRQSCLAAGQATEDCVIGPTLG
                     YSVSYPFGVLGVILAIRLLTSRWGIAFGAARETARQRGESGEPAIEVHAFRVENERLF
                     GTSLRENELGRMTGLVFTRLHHGPFTTLATLETILQQGDLLVGVGSAEAISKATALIG
                     PCTSFPLDELTMHVQYHDL"
BASE COUNT          170 a          316 c          338 g          236 t
ORIGIN      
        1 cggagtctgg tgggcagggg cccgccttgt cagacggaac gctcaccgga accgtcaagg
       61 ccggacactg tcactgctac ggctggtgaa ccaccgtcgc gtgacgctcg gggtaccgtt
      121 tgcgactgtc gcggacgtga agcgatgtcc gccggcaggc gtcgggacag tctgaatgca
      181 tcaactgctg ctttcctcgc cgtacctgtt gttgttcacg gtcatcggcg ccggatacgt
      241 tttgggtcgg atcaacctgg gcgggctgca tttgaacgtc gccgcggttc tctttgtcgg
      301 cctggcattt ggcgcatggg ataagcgttt tcagatacct gagcaggtac agatgctcgg
      361 gttgattctc tacatctatg ccatcgggct gcaggcgggg ccgatcttct tcacagtgtt
      421 ccgtgagcgg gggttgcgat tcaatctgct cgccatcgga accctcgcct tctcctttct
      481 cgtgaccctc gttgtcgggc gcgtgattgg cgttcccagt gacctgctcg ccggcctgtt
      541 caccggcggg ttgaccaata ccccggcttt ggccgctgca accgatgctc tgcggcagag
      601 ttgtcttgct gccggacagg cgacagaaga ctgtgtgatc ggcccgaccc tcggctacag
      661 cgtctcatac ccgttcggcg tactcggcgt gatactcgcc atcaggcttt tgaccagtcg
      721 ctggggcatc gcgttcggag ccgctcgcga gacggcgcgt cagcgcgggg agtccggtga
      781 gccggccatt gaggttcatg cctttcgggt cgagaacgaa cggttgttcg gaacgagcct
      841 gcgggaaaac gaactcggac gcatgaccgg gctggtgttc accagacttc atcatggtcc
      901 cttcaccacg ctggcgacgc tcgagacgat tctgcagcag ggggacctgc tggttggcgt
      961 cggctccgcc gaggcgatca gcaaagcgac ggccctgatc ggaccgtgta ccagtttccc
     1021 actcgatgaa ttgacgatgc atgtgcagta tcacgacctg
//
LOCUS       DAORHD010001726         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_210449_length_1059_cov_2.712151, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001726 DAORHD010000000
VERSION     DAORHD010001726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_210449_length_1059_cov_2.712151"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PKO06_24770"
     CDS             complement(<1..372)
                     /locus_tag="PKO06_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005960802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV72946.1"
                     /translation="MSDKDSVPLPTPEELLALTGTRRCLFGDARQIRQICEIVRKVAR
                     SSGPVLIRGESGTGKEVLASAIHRQSPRADKPFVPLHAGAIPRELFESELFGYLKGAF
                     TGADRDRSGAFDQADNGTLFLD"
     gene            525..>1059
                     /locus_tag="PKO06_24775"
     CDS             525..>1059
                     /locus_tag="PKO06_24775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNV72947.1"
                     /translation="MAEFILNAEVRSKTGKGESRRARSQGMLPSVIYGQGVEKPVHCV
                     FKRRDVEIAFTKIPHRNSIMKIDFAGKANACEVIIRDYQKNPLNHQFEHIDFQAIDRK
                     APLKVEVDINLVGDPIGKKAGAILTTQLKTLRVECLPEKIPAKIDLDVSALDVGDSLH
                     VSDIPKSEIKIISNPKLT"
BASE COUNT          258 a          288 c          297 g          216 t
ORIGIN      
        1 gtcgagaaac agagtgccat tgtccgcctg atcgaaggcc ccggatcgat cccggtcagc
       61 gccggtgaaa gcgcccttga gatacccgaa cagctcactt tcgaacaatt cgcgcggaat
      121 agcgccggca tgaagaggaa caaacggctt gtcggctcgt ggactctgtc gatgaatggc
      181 ggacgcaaga acctccttgc cggtacccga ttcgccgcga atcagtactg gccccgaact
      241 gcgggccacc ttgcggacga tctcgcagat ctgcctgatc tggcgcgcat ctccgaacag
      301 gcagcgtcgt gtcccagtca gggccagaag ctcttcgggg gtcggcaggg gaacggagtc
      361 tttgtcgctc atgcccacga gcataccgca agagcgcgat caatgccaga aggaaggcaa
      421 gcctctctgt caggctcgtt tgggggtgtc ggtattgctg atttgaccac gggttcaaaa
      481 ggtgctacca taacaaaacc aaatcaacct gtaaaggagc ttctatggca gagtttattt
      541 tgaacgctga agtaagaagc aagaccggca agggcgagtc tcgacgcgcc cgcagccagg
      601 gcatgcttcc ctcggttatc tatgggcagg gcgtcgaaaa gcctgttcat tgtgtcttca
      661 agcgccgtga tgttgagatc gcttttacga agattccgca tcgcaacagc atcatgaaga
      721 tcgatttcgc gggcaaagcg aatgcgtgcg aagtcatcat tcgcgattat cagaagaacc
      781 cgctcaatca ccagtttgaa cacatcgatt ttcaggcgat cgaccgcaag gccccgctga
      841 aagtcgaagt ggacatcaat ctagttggcg atccgatcgg caaaaaagcc ggcgccattc
      901 tgaccactca gttgaaaacg ctgcgtgtcg aatgtctgcc ggaaaagatc ccggccaaga
      961 tcgatctcga tgtgtcggct ctcgatgtcg gcgattcact gcacgtgtcc gatattccga
     1021 agagtgaaat caagatcatc tcgaatccga agctgacgg
//
LOCUS       DAORHD010001727         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_211832_length_1055_cov_3.666000, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001727 DAORHD010000000
VERSION     DAORHD010001727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_211832_length_1055_cov_3.666000"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKO06_24780"
     CDS             complement(<1..476)
                     /locus_tag="PKO06_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72948.1"
                     /translation="MKMRLLLVIWMVLGLVSVGWSGPANSAHIAENAVWVGHAGVEKM
                     CALPLVAECPFMSGTETKSARHLQAFIEKTGLDPRKDVRAVTAWATQYEGDVGVMIIY
                     AQHVDRARLVAFLREKRSEVKEETVSGYDLYTWNMRYMRKQLEVTGTFVDDQTILVG"
     gene            complement(505..>1055)
                     /locus_tag="PKO06_24785"
     CDS             complement(505..>1055)
                     /locus_tag="PKO06_24785"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNV72949.1"
                     /translation="AQHHLENAAKAESQEICFIPENDYLEFLRRFLPPDHPGFQNGDL
                     VDATGTVIGRHQGYLGYTIGQRKGLGGGHGRRLFVTGIDAARRRVFVGEESELVCPRL
                     RVRGMNVLAPEAVTRGETYQIQFRHRSKPVPAQLKEMTDECWSLEFLETAKSVTPGQS
                     AVLYQGDLVVAGGRIAELVTPR"
BASE COUNT          237 a          338 c          253 g          227 t
ORIGIN      
        1 ccaaccagaa tcgtctggtc atcgacaaac gtaccggtga cttcgagctg cttgcgcatg
       61 tagcgcatgt tccaggtgta aagatcataa ccggagaccg tttcttcctt tacctctgag
      121 cgtttttcac gcagaaaagc caccagtctg gctcgatcga cgtgctgcgc atagatgatc
      181 atgacgccga cgtcgccctc atactgcgtc gcccaagccg tcaccgcacg tacgtccttg
      241 cgcggatcaa gaccggtttt ctcgataaac gcctgcaggt gtcgagcgct tttcgtttcg
      301 gttccgctca tgaacggaca ctcggcgacc aacggcaacg cgcacatctt ttccacaccg
      361 gcatgtccca cccacaccgc attttcagcg atatgcgcag aattcgccgg tcccgaccat
      421 ccgacgctca ccagtcccag aaccatccag atcaccagca acagccgcat cttcatacga
      481 tcctccttac aggaaattca cctctcaccg cggggtgacc agttcggcaa tgcgcccgcc
      541 agccaccacc agatcacctt gatacaggac agcggactgt ccgggagtga cagatttcgc
      601 cgtttcgaga aactcgagac tccagcattc atcagtcatt tccttcagtt gcgctggtac
      661 cggtttcgat cggtgacgaa actgtatctg ataggtctca cctcgagtca ccgcttcggg
      721 cgcgagcacg ttcattcccc tcaccctcaa gcgagggcaa accagttctg attcctcgcc
      781 aacaaataca cgacgacggg cggcatcgat gccggtgacg aagaggcgtc ggccatggcc
      841 tccacccaac cctttccgtt gaccgatcgt gtagcccaga tacccctggt gcctgccgat
      901 gactgtaccg gtcgcatcaa ccaaatcacc gttctgaaag ccaggatgat cgggcggcaa
      961 aaagcgtcgg agaaactcca gataatcatt ttcggggatg aaacagattt cctgagattc
     1021 tgctttagcg gcattctcca ggtgatgctg tgccg
//
LOCUS       DAORHD010001728         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_212171_length_1054_cov_3.817818, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001728 DAORHD010000000
VERSION     DAORHD010001728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212171_length_1054_cov_3.817818"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PKO06_24790"
     CDS             complement(<1..976)
                     /locus_tag="PKO06_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72950.1"
                     /translation="MRRHAHRLVCALCVSLLLVLASDVSLRAAPKIPLELEEIREDIS
                     LLNLLRGLYLSKDQLGQLLDLANQAQRIRQSTLQPFLDRKDQIIGDFRSLRDSLYLAP
                     GNEKKAQEKAREVDQQMKEAVGGVNDLIYALEEKASSILTSAQICILDDFKACLIPPK
                     DLGNPVRVGQAGAAEGLLGKLTDLIHCAPDRLWKERGDRLLKRVSEKLEEKSGEISPA
                     LKQDLNRRLHEIANKIRACNDVDFTLKKHDLAKELLVINPDKGLKHGHRKNGPVARWL
                     LADVTVRVLPQWLKAMERVDFTAAGDDATDPPLALEKTEALARLLVTLR"
BASE COUNT          212 a          297 c          315 g          230 t
ORIGIN      
        1 gtcgcaaggt aaccaacaga cgtgccagtg cttcggtttt ttcgagagcc agcggcggat
       61 cggtcgcgtc gtcgcccgcg gcggtgaagt ccacacgttc cattgctttg agccactggg
      121 gcagcacacg aacggtgacg tccgcgagca gccagcgggc aaccggacca ttcttgcgat
      181 ggccgtgctt caagcctttg tccgggttga tgacgagcag ttctttggcc agatcatgtt
      241 tcttcaaggt gaaatcgaca tcgttgcagg ctcggatctt gttcgcgatc tcatgcaggc
      301 ggcgattcag atcctgtttc agcgcggggc tgatctctcc ggacttttcc tcgagtttct
      361 cagaaacgcg ctttaacagc cgatctccgc gttccttcca cagccggtcg ggtgcgcagt
      421 gaatcagatc ggtcagcttt cccagcagac cttcggcggc gcccgcctga ccgacgcgta
      481 ccgggttgcc caggtccttc ggcggaatca gacaggcttt gaaatcatcg agaatgcaaa
      541 tctgagccga tgtcaggatc gacgaggcct tttcctcgag agcgtagatg agatcgttga
      601 ctcccccgac ggcttccttc atctgctggt cgacttctcg agccttttcc tgggccttct
      661 tctcgttccc gggagcgaga tacaggctgt cgcgcaacga acggaaatcg ccgatgatct
      721 gatccttgcg gtcgagaaac ggctgaaggg tgctttggcg aatccgctga gcctggttcg
      781 ccaggtcgag caattgtccg agttgatctt ttgaaagata gagtccgcga agcaggttga
      841 gcagggaaat atcttcgcgg atctcttcga gttccagcgg aatcttcggc gccgcgcgca
      901 gcgacacgtc agaagccagg acgagcagca gtgagacaca gagagcgcac acgagacggt
      961 gagcgtgacg acgcatgatg aaacctcctc gagatgtcag ggttgccagt gaccgggaat
     1021 cttctgagta cagtccggac aagcgccgga aacc
//
LOCUS       DAORHD010001729         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_212936_length_1052_cov_3.561685, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001729 DAORHD010000000
VERSION     DAORHD010001729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212936_length_1052_cov_3.561685"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="PKO06_24795"
     CDS             <1..796
                     /locus_tag="PKO06_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72951.1"
                     /translation="GTSRERFTIGTTIQLVDEVCAVWGNRAWDYLIGALTAGAPKLFL
                     MILLFYIDVDRRGFGSAMTLDPWLLFALILVYPLFFRPFAWELLGKTFLSGWPERSIR
                     QRFQRRLTFGSYTLLLRRNLFPAMINVLLLGLFLVVPSTNLNLEVLYLGLILFFWVIP
                     LNFCRLPLTLLDPLTPTSVEHASELGSAHAPTIRSSLTDFTWFIAYWYLAIAISFVFV
                     ASYFVRSTSGMHSLCGVLLADILLDPLWIGFHLTLTYLLKQQKAAA"
     gene            793..>1052
                     /locus_tag="PKO06_24800"
     CDS             793..>1052
                     /locus_tag="PKO06_24800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72952.1"
                     /translation="MRFRATVLSLILLLASVSLLAESLPDFSRRVLALRGRTPTLETA
                     KYVLDSTTEGLMTDLSLINEVSTPGGAIGIELSWINDIVQDLR"
BASE COUNT          170 a          341 c          259 g          282 t
ORIGIN      
        1 gggaaccagc cgggagcgtt tcacgatcgg gacaacaatc cagctggtcg atgaagtctg
       61 cgccgtctgg ggcaatcgtg cctgggatta cctgattggt gcgctgactg ccggcgcacc
      121 aaagctgttt ctgatgattc tgctgttcta catcgacgtg gacaggcgtg gattcggcag
      181 tgcgatgacc ctcgacccct ggctgctgtt cgccctgatt cttgtctatc cgctgttttt
      241 ccgtcccttc gcctgggaac tcctcggcaa gacatttcta tccgggtggc cagaacgttc
      301 catcagacag cggtttcagc gtcgcctgac ttttggttcc tacaccctgc tgctccgtcg
      361 caacctgttc cctgccatga tcaatgtcct acttctggga ctctttctcg tcgttccctc
      421 taccaacttg aatcttgagg ttctgtatct cggactgatc ctcttcttct gggtgattcc
      481 cctgaacttc tgtcgattgc cactcacctt actggacccc ctgaccccga cctccgtcga
      541 gcacgcgtcc gagttaggct ccgcccatgc acccaccatt cgcagctcgc tcaccgactt
      601 cacgtggttc atcgcctact ggtacctggc catcgccatc tcgttcgtct tcgtggcttc
      661 gtatttcgtc cgctcgacca gcggcatgca ttctctctgc ggtgttttgc tggctgacat
      721 tctgctcgac cctctctgga tcggctttca tctcacgctg acgtatctgc tcaagcaaca
      781 gaaggccgcc gcatgagatt ccgcgcgact gttctgtcac ttatcctgtt actcgcgagc
      841 gtttcgctcc tggccgagtc tctgccggat ttctcacgcc gggtcctcgc cctgcgaggt
      901 cgcactccga cactcgaaac agcgaagtac gttctcgatt ccaccaccga aggcctgatg
      961 acagatctga gtctcatcaa tgaggtgtcg actcccggtg gcgcaatcgg aatcgagctt
     1021 tcgtggatca atgacatcgt ccaggatctg cg
//
LOCUS       DAORHD010001730         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_212973_length_1052_cov_3.026078, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001730 DAORHD010000000
VERSION     DAORHD010001730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212973_length_1052_cov_3.026078"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKO06_24805"
     CDS             <1..412
                     /locus_tag="PKO06_24805"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNV72953.1"
                     /translation="EIFLTNSVNVQKSYWQSPHLKQENIEEALTAGLEQAGDTYRPRL
                     HWRRFFRELVEQDLARLARDTRALLASPADAVTFPLPTTEPTTIVIGPETGFSPDETR
                     TFLEAGFAPCSLGPRILRVETALPALVSRLVPSL"
     gene            complement(419..>1052)
                     /locus_tag="PKO06_24810"
     CDS             complement(419..>1052)
                     /locus_tag="PKO06_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HNV72954.1"
                     /translation="KHGPNTILGVNTVFGMDSVQALLRTFCDAMKFSLDQPEIAGADG
                     QTLHRLFRSVVEYALQDVPPHYLNPSEKQVFERIFSEHNFFRSLYRNYPLVFLTSPQE
                     TDINLTISQINTHKIRGADVFMIAEDNEHLREAVSMRPAGDDRYRHGYVTLPRTDSPI
                     LAIFSMTVVLQMLALQMSRRKMEFLDRLEINEHGVHPDVPKNVSKSITVD"
BASE COUNT          213 a          300 c          314 g          225 t
ORIGIN      
        1 ggagatcttc ctgaccaact cggtcaatgt ccagaaaagc tattggcaaa gcccgcatct
       61 gaagcaggag aacatcgagg aagccctgac cgcggggctg gaacaggccg gtgacacgta
      121 tcgaccacgc ctgcactggc ggcggttttt ccgcgaactg gtggaacagg atcttgcacg
      181 tctggcgcgc gacacccgcg cattgcttgc ttctcccgcc gacgccgtca cttttccgct
      241 gcccaccacg gaaccaacga ccattgtcat cggccccgag acagggtttt cgccagatga
      301 aacccggaca tttctcgaag ccgggttcgc accctgttct ctcggcccgc gcattctccg
      361 cgtcgagacc gcgttgcccg ctctggtcag tcggctggtt ccgtctctct gatcaccgtc
      421 agtcgacggt aatcgatttc gagacgttct tgggaacgtc aggatggacg ccgtgttcgt
      481 tgatttcgag acggtccaga aactccatct tccggcggct catctgcagc gccagcatct
      541 gcagcacgac ggtcatcgag aagatcgcca gaatcggtga gtcggtgcgg ggcagagtca
      601 cgtagccatg tcggtaccga tcgtcgccgg ccggtcgcat tgaaacggct tcgcgcagat
      661 gctcattgtc ttccgcgatc atgaatacat cggccccgcg gatcttgtgg gtattgatct
      721 gcgagatggt gagattgatg tcggtttcct gcggactggt cagaaagacg agcgggtagt
      781 tgcgatacag actgcgaaag aaattgtgtt cggaaaagat gcgttcgaac acctgcttct
      841 ctgaggggtt caggtagtga ggcggtacat cctgcagggc gtactcgacc accgagcgaa
      901 acaaccggtg cagagtctgg ccatcagccc cggcgatttc gggttgatcg aggctgaact
      961 tcatggcgtc gcagaacgtg cgcaacaggg cttgtacgga atccatgccg aagaccgtgt
     1021 tcacgccaag aatcgtgttg ggaccatgtt tg
//
LOCUS       DAORHD010001731         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_212987_length_1052_cov_2.876630, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001731 DAORHD010000000
VERSION     DAORHD010001731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212987_length_1052_cov_2.876630"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PKO06_24815"
     CDS             complement(<1..386)
                     /locus_tag="PKO06_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020468908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV72955.1"
                     /translation="MKKYVYSFAGGKADGNASMKELLGGKGANLAEMCVIGLPVPAGF
                     TVTTECCVEYFDLGGKYPKELEKQVEEGLKAIEKAMGMKFGDTENPLLLSCRSGARKS
                     MPGMMETVLNVGLTTKTIPALIKKTN"
     gene            complement(569..973)
                     /locus_tag="PKO06_24820"
     CDS             complement(569..973)
                     /locus_tag="PKO06_24820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72956.1"
                     /translation="MDNHESGGQRKKIFILDDEEPIILLLKTILNVYGYDGCESSSPQ
                     EAVARVIQEKPDLVILDIAMPEMDGYQVCRQLKANPETQKIPVVMITALALEQDRKMA
                     MDAGADGFILKPFDPRDVVAEIQRLTREPKNK"
BASE COUNT          240 a          287 c          266 g          259 t
ORIGIN      
        1 ttgttggtct ttttgatcag agccggaatc gtcttggtcg tcagaccgac gttgagaacg
       61 gtttccatca tgcccggcat ggacttgcga gcaccggaac gacaagagag caacagcggg
      121 ttctcggtgt caccgaactt catgcccatg gctttttcaa tcgccttcag gccttcttcg
      181 acctgctttt cgagttcttt aggatatttg ccgccgagat caaaatactc gacgcagcat
      241 tcggtggtaa cggtgaagcc ggcgggaacc ggcagtccga tcacgcacat ttcggcgagg
      301 ttcgcgccct tgccgccgag cagttctttc atgctcgcgt tgccatcggc cttgccacca
      361 gcaaaagagt acacatactt cttcatcacg gggcctccgt tgtagagatt tcgcaaaagg
      421 ttcgcctttg cagttgccgc agaggatatc acgtcgacag cctgaattca agcgggtacg
      481 tgagaaaatc aaaagaaaat tccctgaaat gatcagcatt tcagacaaag cggaatggga
      541 accccctgat ggggaaagga acggccggtt acttattctt cggttcgcgg gtcagacgct
      601 gaatttcggc caccacatcg cgcggatcaa acggtttcag aatgaaacca tcagcaccag
      661 catccatggc catcttgcga tcttgttcca gcgcaagtgc ggtaatcatg accacgggaa
      721 tcttctgcgt ttcggggttc gctttcagct ggcggcacac ctgatatccg tccatctcgg
      781 gcatggcgat gtcgagaatc acgaggtccg gcttttcctg aataacgcgc gcaaccgctt
      841 cttgcgggga tgacgactcg cacccgtcat atccgtacac attcagaatg gtcttgagaa
      901 gcagaatgat cggctcttca tcatcaagaa tgaagatctt cttgcgctgg ccgcctgatt
      961 cgtggttatc catgaaacac cttacaacag ctcgttccaa atacgatatg gttctttact
     1021 accacgggtt tctctcacct gtcaacgcgt tg
//
LOCUS       DAORHD010001732         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_213052_length_1052_cov_2.480441, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001732 DAORHD010000000
VERSION     DAORHD010001732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213052_length_1052_cov_2.480441"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKO06_24825"
     CDS             complement(<1..329)
                     /locus_tag="PKO06_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72957.1"
                     /translation="MSAKNVPMNEQAICADPEAVLSYHAAEPSGKITVCPSKPCSTRE
                     ELSLAYTPGVAVPCLRIQKNPEAVWEYTARGNLVAVITNGTAVLGLGDIGAAAGKPVM
                     EGKAVLF"
     gene            472..>1052
                     /locus_tag="PKO06_24830"
     CDS             472..>1052
                     /locus_tag="PKO06_24830"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HNV72958.1"
                     /translation="MSAPARVGTISQYLAVGLGALAVFGLLVGTLFSLTRAYVLSKAE
                     DEIEGMLLQHKGVHHYVQRKNHPELYRFIASGSIKPEAYTPVLFSSSFMVRNMHVFYN
                     EEREKAGMPPLYYKMAASNPRNPVNTADEYEARLIAFFNTHPEEKEYREIKEVDGKQY
                     LYVAIPFLRNDRNCLKCHGKREESPPGLQALYPG"
BASE COUNT          235 a          294 c          279 g          244 t
ORIGIN      
        1 ttgaacagca cggcttttcc ttccataacc ggcttgccgg cggccgcacc gatgtcaccg
       61 agccccagca ccgcggtacc gttcgtgatg acggcgacga gattgccacg cgcggtgtat
      121 tcccaaaccg cttctgggtt tttctgaatg cgcaggcagg gcacagccac gccaggcgta
      181 taggccaggc tgagttcttc acgcgttgaa cagggtttcg aggggcatac tgtgattttg
      241 ccgctcggtt cagccgcatg ataggacaac accgcctcag ggtcagcaca gatcgcctgc
      301 tcgttcatcg gaacgttttt cgcgctcatt gaagtcctcc actgcccgcc agtcggacac
      361 atgtttcggg tatgatcatt cctgttgcta cctacgggac actgcgatta tagtagaagt
      421 gtagattgtt ttcattcgtg agtgatcaac tgtagataga ggggcatatc tatgtctgca
      481 cctgcgcgtg tcggcaccat ttcgcagtat ctggctgtcg ggctcggcgc attggcggtt
      541 ttcgggttgc tcgtcggaac cctgttttcg ctgaccagag cctacgtgct cagcaaggcc
      601 gaagacgaga tcgaaggaat gctgctgcag cataagggag tgcatcatta cgtacagcga
      661 aaaaatcatc ctgaactgta tcgtttcatc gcgtccggtt ccatcaagcc ggaagcctat
      721 acgccagtcc tcttctcctc ttcgttcatg gtgcgcaaca tgcatgtctt ctataacgaa
      781 gaacgcgaga aggcaggaat gccgccactc tattataaaa tggcggcctc gaatccacgc
      841 aacccggtca acaccgctga cgaatacgaa gctcgtctga tcgccttttt caacacccat
      901 cccgaagaaa aagaatatcg tgagatcaag gaagttgatg gcaagcagta tctgtacgtc
      961 gcgattccgt ttctgcgaaa cgaccggaac tgcctgaaat gtcacggcaa gcgggaagaa
     1021 tcgccaccgg gtctgcaagc cctctacccg gg
//
LOCUS       DAORHD010001733         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_213325_length_1051_cov_3.165663, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001733 DAORHD010000000
VERSION     DAORHD010001733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213325_length_1051_cov_3.165663"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1051
                     /locus_tag="PKO06_24835"
     CDS             <1..>1051
                     /locus_tag="PKO06_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNV72959.1"
                     /translation="YARQFLQRMEKPEVDLIEGLSPAISIEQKATSHNPRSTVGTVTE
                     IHDYLRLLFARVGTPYCPEHQQPLEAQTVSQMVDHVLALPEGSRLMILAPVVTNRKGE
                     QLELIAELRAQGFARLRIDGQIYEIDAVPKLAKTHKHSVDVVVDRLKIRDDIRQRLAE
                     SFETALRHADGRAIAYEMDNEREHFFSARFACPVCSYSLSELEPRIFSFNSPLGACPK
                     CDGLGVIQFFDPKRIVSHPERSLAAGAIRGWDRRNPFYFQMLCSLAAYYAFAIDTPFN
                     ELPENVQQILLYGSGRVQVPFNYVNERGQMTLREHAFEGIVVNLERRYKETDSLAVRE
                     DLARLISNQTCPTCQG"
BASE COUNT          203 a          334 c          290 g          224 t
ORIGIN      
        1 ctatgcgcgg cagtttctgc agcgtatgga gaaacccgag gtcgatctga tcgagggact
       61 ttccccggca atctcgatcg agcagaaagc gaccagtcac aatccgcgtt cgacggtcgg
      121 cacggtcacc gaaatccacg actacctgcg tctgctcttc gcccgcgtcg gcacgccgta
      181 ctgtcccgaa caccagcagc cgctcgaagc gcagacggta tcgcagatgg tcgatcatgt
      241 tctggccttg cccgagggga gccggctgat gatcctcgca ccggtggtca ccaaccgcaa
      301 gggcgagcag cttgagctga ttgccgagtt gcgggcacag ggttttgccc gcctgcggat
      361 tgacggccag atttacgaga tcgatgccgt tcccaaactc gccaagacac acaaacacag
      421 cgtcgacgtg gtcgttgacc ggctcaagat tcgtgacgac atccgccagc gccttgccga
      481 gtcttttgaa accgcgctgc gccacgccga tggccgcgcc attgcctacg agatggacaa
      541 cgaacgcgag catttcttct ctgcccggtt tgcctgtccg gtctgttcgt actcgctatc
      601 cgaactcgaa ccccggattt tctcgttcaa cagcccactg ggtgcctgcc cgaagtgcga
      661 cggccttggc gtcatccagt tttttgatcc gaaacgcatt gtgtctcatc cggaacggtc
      721 tctggctgcc ggcgccattc gcggctggga ccggcgcaat cccttctact tccagatgct
      781 ctgctcgctc gccgcatact atgcctttgc gatcgatact ccgttcaacg aactgcccga
      841 gaacgtccag cagattctgc tttatggttc ggggcgcgtg caagtgccgt tcaactatgt
      901 caacgaacgt ggtcagatga ccctgcgcga gcatgctttt gaaggcatcg tggttaacct
      961 cgaacgccgt tacaaggaaa ccgactcact cgccgttcgt gaggatctgg cgcgattgat
     1021 cagcaatcag acctgcccga cctgccaggg c
//
LOCUS       DAORHD010001734         1051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_213500_length_1051_cov_2.034137, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001734 DAORHD010000000
VERSION     DAORHD010001734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213500_length_1051_cov_2.034137"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..>1051
                     /locus_tag="PKO06_24840"
     CDS             122..>1051
                     /locus_tag="PKO06_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015812982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV72960.1"
                     /translation="MNTALVTGSAGLIGAESVRFLSRKGFKKVVGIDNDLRKYFFGDE
                     ASTDWNRRRLQEEWPGYEHHAIDIRDVSALEKIYRTYGQTLDLVIHAAAQPSHDWAAR
                     EPFTDFTVNANGTLNLLEMTRLYCPDAVFIFTSTNKVYGDTPNRLPLVEKEQRWELEE
                     SHPWYTHGIDESMSIDQSKHSIFGASKASADLMVQEYGRYFGIKTGCFRGGCLTGPDH
                     SGTQLHGFLAYLMKCAVTSHPYTVFGYKGKQVRDNIHSHDLVNMFWHFFQNPRAGEVY
                     NAGGGRHSNCSLLEAINLCQEITGRKMNWSYSET"
BASE COUNT          255 a          280 c          295 g          221 t
ORIGIN      
        1 cattggaagc cggaacgaac accggcacag atcgtcgagg aaatcgtaca atggatatca
       61 gatcatcgtt ctgttctgca gccgattctt gccgcatgat cagctctcgg caaaggctgc
      121 catgaatacc gctctcgtga caggttccgc cggactcatc ggcgctgaaa gtgtacgttt
      181 cctctcacgg aagggcttca agaaggtggt cggcatcgac aacgacctgc gaaagtattt
      241 cttcggcgac gaagcgtcga cggactggaa ccggagaagg ctgcaggaag aatggccggg
      301 gtatgaacat cacgcgatcg atatccgcga tgtgtcggcg ctggagaaga tctaccggac
      361 ctacggtcag actctcgatc tcgtgatcca cgcggcggcc cagccctcgc acgactgggc
      421 ggcacgtgaa ccgttcacgg acttcacggt gaatgcgaac ggtacgctga acctgctcga
      481 gatgactcgt ctgtattgtc ccgacgctgt tttcatcttt acctcgacca acaaggtgta
      541 tggagacact ccgaaccgtc tcccgctggt ggaaaaggaa cagcgttggg aactggagga
      601 gtcacacccg tggtacacgc atggcatcga tgaaagcatg agtatcgatc agtcgaagca
      661 cagcatcttc ggtgcttcaa aagcttctgc agacctcatg gtacaagaat acggccggta
      721 cttcgggata aaaaccggtt gttttcgcgg tggctgtctg accggccccg atcactcagg
      781 aacgcagttg cacgggtttc tggcctatct gatgaaatgc gccgtcacca gccatccgta
      841 caccgtcttc gggtacaaag ggaaacaggt tcgagacaac atacacagtc atgatctggt
      901 caatatgttc tggcactttt ttcagaaccc gcgagcggga gaagtgtata atgcgggcgg
      961 cggtcgtcac tcgaactgct ctctgctcga agccatcaat ctgtgccagg agataaccgg
     1021 cagaaagatg aactggagct attcagagac t
//
LOCUS       DAORHD010001735         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_213702_length_1050_cov_2.873367, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001735 DAORHD010000000
VERSION     DAORHD010001735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213702_length_1050_cov_2.873367"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PKO06_24845"
     CDS             complement(<1..321)
                     /locus_tag="PKO06_24845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV72961.1"
                     /translation="MSGEFLLDTNIVIALFADEPLLMKRLTPNRKIFVSTIVLGELFY
                     GAHKAARSAENLKRISEFAEKSAIVVPDYETADSFGQIKSRLLSKGRPIPDNDIWIAA
                     TAIQH"
     gene            complement(311..541)
                     /locus_tag="PKO06_24850"
     CDS             complement(311..541)
                     /locus_tag="PKO06_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72962.1"
                     /translation="MKSTALKKEIVADLNRLPIDRQQQVRDFVKALIVSLPRGTPASE
                     LKVFAGTISKKDLDAMANAVKEGCEQVFPSEW"
     gene            complement(584..>1050)
                     /locus_tag="PKO06_24855"
     CDS             complement(584..>1050)
                     /locus_tag="PKO06_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002646161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HNV72963.1"
                     /translation="RVSSGCIRMFPEDITRIFGEFREVTRDGVPISRIFKNEDFGYRT
                     ITVERPQRDEHGKVVLSTKGKTKGKPVPDANLRDTENVPLSEDVEVYFKREVLPHVAD
                     AWIDEEKTKVGYEIPFNRHFYVFKPPRPLAEIDAELKIVTDRILTMIGELSK"
BASE COUNT          217 a          289 c          288 g          256 t
ORIGIN      
        1 gtgctgaatc gcggttgcgg cgatccagat gtcgttgtct ggaatcgggc ggcctttgct
       61 gagaaggcgg gatttgatct gtccgaacga gtcggcggtt tcgtaatcgg ggacgacgat
      121 tgccgacttt tccgcgaact cagagattcg tttgagattc tctgccgatc tggccgcctt
      181 gtgggcaccg taaaacaatt cccccaagac gatcgttgac acaaagattt ttcgattcgg
      241 ggtcagtctt ttcatgagca gaggctcgtc agcgaacaac gcaatgacga tgttggtgtc
      301 gagaagaaac tcaccactca ctggggaaca cctgctcaca gccttccttc acggcattcg
      361 ccatcgcgtc gagatctttc ttggaaatgg ttccagcgaa gactttcagc tccgatgccg
      421 gggtgccgcg aggcaaagac acaatcaagg ctttaacaaa atctcgaacc tgctgttggc
      481 ggtcgatcgg aagcctattc agatccgcaa cgatttcttt cttcaaagct gtcgacttca
      541 tgcgtttcac tcccaatcag gatttacggc agtatagcaa agttcacttc gacagttcgc
      601 cgatcatggt cagaatgcgg tcggtgacga tcttcagttc ggcgtcgatc tcggcgagcg
      661 ggcgaggcgg tttgaacacg tagaagtgtc ggttgaacgg gatctcatac cccaccttcg
      721 tcttctcctc gtcgatccac gcgtcagcga cgtgcggcag aacctcacgc ttgaaataca
      781 cttcgacatc ctcgctcaac ggcacatttt cagtatctcg caggttggca tctgggacag
      841 gcttcccttt ggtcttgccc ttcgtgctga gaaccacctt gccgtgctca tctcgctggg
      901 ggcgctcgac cgtgatagtg cggtagccga aatcctcgtt cttgaagatg cgactgatcg
      961 gcactccgtc tcgcgttacc tcgcggaatt caccgaagat gcgggtgatg tcctcgggga
     1021 acatgcggat gcagcccgag ctgacgcggc
//
LOCUS       DAORHD010001736         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_213707_length_1050_cov_2.830151, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001736 DAORHD010000000
VERSION     DAORHD010001736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213707_length_1050_cov_2.830151"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1050
                     /locus_tag="PKO06_24860"
     CDS             17..>1050
                     /locus_tag="PKO06_24860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV72964.1"
                     /translation="MGTIGADLIISSWLDWLHALKVSDDGYLFVLDAEGRPLMFPARA
                     IQDLVGDPAQKRALLEAIKPIASQTWNPELCAALQQSLPQVATGELQLLFARMQRQET
                     GVIQHSGTEQNIIAYAPVGAASWSVGICMPLIKTLSISETARRRMDEATSLYGKFFVG
                     VLIFAAFVSGLIGFWSSRAISRPILGMIASIRELSRGGEWKPLVAETSDELRVLADSA
                     NVMVRTLRDNEKLLADIFNGVSDAIFLHDGETGKILRVNRKMCEMFKCTEEQALSASL
                     DQLTAGIEPYNAAQAREHIKQALNGHLPPFQWRSRDYHGNVFWTEVSLTRAQTGDLVV
                     ILAVVRDISER"
BASE COUNT          220 a          289 c          317 g          224 t
ORIGIN      
        1 tgacggagat cgcttcatgg gaaccattgg cgctgatctc atcatttcct catggcttga
       61 ctggttgcat gcgctgaaag tcagcgacga cggatatctg ttcgtactcg atgccgaggg
      121 acgaccgctg atgttccctg caagggcgat tcaggatctg gtcggcgacc cggctcagaa
      181 gcgagcgttg ctcgaagcca tcaaaccgat cgccagtcag acgtggaacc ctgaactgtg
      241 cgcggctctg caacagtcac tgccgcaagt cgcgaccggc gagctgcagc tgctgtttgc
      301 ccgcatgcag cgacaggaga ccggggtaat ccagcattcc ggaaccgagc agaacatcat
      361 cgcgtatgca ccggtcggag ctgccagctg gagcgtcggc atctgcatgc cgttgatcaa
      421 gactctcagt atctcggaga ccgctcgacg gcgcatggat gaagcgacga gtctctatgg
      481 aaagttcttt gtcggggttc tgatcttcgc cgcgttcgtg tccggtctga tcggcttctg
      541 gagcagtcgc gccatcagtc gccccattct gggaatgatc gcgtcgattc gcgaactcag
      601 tcgcggcggg gaatggaaac ctctcgtggc cgaaacgagt gacgagttac gggttctggc
      661 cgacagcgcg aatgtcatgg tgcgcaccct gcgtgacaac gagaagcttc tggctgacat
      721 cttcaacggc gtcagcgatg ccattttcct gcatgacgga gagacgggaa aaattctgcg
      781 ggtgaatcga aagatgtgcg agatgttcaa gtgcaccgaa gaacaggctc tctccgcgtc
      841 tcttgatcag ctgactgctg gcatcgaacc atacaatgcg gcgcaggccc gcgaacacat
      901 caagcaggct ctgaatggcc atctgccacc gtttcagtgg cgtagtcgtg attatcacgg
      961 aaatgtgttc tggaccgaag tcagtttaac ccgtgcgcaa accggcgacc tggtggtgat
     1021 tctcgctgtg gtgcgcgata tctcggaacg
//
LOCUS       DAORHD010001737         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_214061_length_1049_cov_2.914487, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001737 DAORHD010000000
VERSION     DAORHD010001737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214061_length_1049_cov_2.914487"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKO06_24865"
     CDS             <1..457
                     /locus_tag="PKO06_24865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72965.1"
                     /translation="PAIGRLFFEKAITCAQKALEISPDELILLDQLFTCMMYLRRTED
                     AGKIREQILLVSPNFLTTWERADDQSWCFIASYAYQDTPEHIELFREFRRRCLLPWLP
                     GRAVNGLYIRISPLLVLLARRGGIPAPAVRRLLEPLRRLLSRAYSRHTR"
     gene            487..>1049
                     /locus_tag="PKO06_24870"
     CDS             487..>1049
                     /locus_tag="PKO06_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HNV72966.1"
                     /translation="MTSTIPIEVPLAAGRQIIDLNNLFINAADPLRYVRIAEAAGFPR
                     NCCAWIRGLYGLIQDLRIRRVIFVTGGDCSNTHALMETLKPDLSEMLTFSYPYPGEKE
                     ALSREIDRFCSAFAITRTQAEQKGTELSKIRQMLAELDRLTWQTDTISGEDNFRWLVS
                     SSDFQGNPATYQSALSLFRCLKGRPGSG"
BASE COUNT          203 a          323 c          266 g          257 t
ORIGIN      
        1 gccggcaatt ggcaggctgt tcttcgaaaa ggcgattacc tgtgcccaga aagccctcga
       61 gatctcacct gacgagctca ttctgctcga tcagctgttc acctgcatga tgtatctgcg
      121 gcgtacagaa gatgccggta agattcgcga acagattctt ctcgtcagtc ccaactttct
      181 gaccacctgg gaacgcgccg atgaccaaag ttggtgtttc atcgcctcgt acgcttatca
      241 ggacacaccc gagcacatcg aactctttcg cgaattccga cgccgctgtc tgctgccatg
      301 gctgcctggg cgtgcggtca acggactcta catcagaatc tcccccctgc tcgttcttct
      361 cgcgcggcgg gggggtattc cggcacctgc cgttcgtcgg ctgctcgagc cgctccggcg
      421 actgctcagc cgcgcatact cccgtcacac gcgatgaacc ccgtctttcc accggaagcc
      481 cttggcgtga cttccacgat tcccatcgag gttccgctcg ctgctggtcg ccagatcatc
      541 gatctgaaca atcttttcat caacgctgcc gatcccttgc ggtatgttcg aattgctgaa
      601 gcagccggtt tcccccgcaa ctgctgtgcc tggattcgtg ggttgtatgg tctgatccag
      661 gatctccgca ttcgtcgagt gattttcgtc accggtggtg actgttcaaa cacccatgcg
      721 ctcatggaaa cgctcaagcc tgatctttcc gaaatgctga ctttttcgta tccctatcca
      781 ggcgagaaag aggcgctttc tcgcgaaatc gatcgattct gttctgcctt tgcgatcacc
      841 agaacccagg ctgaacaaaa gggaaccgag ctctcaaaga ttcggcagat gctggctgag
      901 cttgatcgcc tgacctggca gaccgatacg atctccgggg aggacaactt tcgatggctg
      961 gtgtcttcat ctgattttca ggggaatccc gcgacgtatc aatcagccct ttcactcttc
     1021 cgctgcttaa aagggcgacc cggctcagg
//
LOCUS       DAORHD010001738         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_214798_length_1047_cov_3.077621, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001738 DAORHD010000000
VERSION     DAORHD010001738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214798_length_1047_cov_3.077621"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..>1047)
                     /locus_tag="PKO06_24875"
     CDS             complement(133..>1047)
                     /locus_tag="PKO06_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72967.1"
                     /translation="LEKVATVEADEECQDLLQQAIDTVRARLARAPATVETTEPVAQP
                     LSPDHFVTLSPQERATILTRVSPEQRRLSLALAPELLRRESHPLIAARIVRLFGADWP
                     EPQLSELTARLDVSSTALRMAIIDTLVRRAPDLLKPRLAELLTAADPMIRSQAIRVLI
                     RVDKDEAIEQLRALLLSPERALRLAGLQNCLYLPFPEIQDLMIAYLAAETDSELIERA
                     GLLFAINPDPRLPFHLYELASTATGTKSHSLRQVFEIACQLLKDSNILGDRFQAYEVK
                     LQNWIAMKAAHRFVQQCLDQLPAPPETW"
BASE COUNT          203 a          270 c          354 g          220 t
ORIGIN      
        1 gagataggta ttcaagagag tttcgatggc gaggcgtgct tcgtgagaaa tcggccactc
       61 cagagacttc tggaaagcca cgaggacggg cggcttttcc agttcgcgac gagtcgtggt
      121 gatcatttca tctcaccagg tctcgggcgg ggcgggcaat tgatcgagac actgctggac
      181 aaatcgatga gccgctttca tggcgatcca gttctgcagc ttgacttcgt aggcctggaa
      241 tcgatccccc aggatgttcg agtctttgag aagttggcag gcgatctcga atacctgccg
      301 caatgaatgg ctttttgtac ctgtcgccgt tgatgcgagc tcgtacaaat gaaatggcag
      361 gcggggatcg gggttgatgg caaacagtaa cccggcgcgt tcgatgagtt cgctatctgt
      421 ctcggccgcc aggtaagcga tcatcaggtc ctgaatctct ggaaacggga gatacagaca
      481 gttctgtagc ccggcaaggc gcagggcgcg ttctggagag aggaggagag cacgcagctg
      541 ctcgatggct tcgtctttat cgacgcggat caggacacga atggcctgtg accgaatcat
      601 ggggtcggcg gctgtcagca gctcggccag gcgcggcttc aggagatcgg gagcccggcg
      661 caccagggta tcgatgatcg ccatgcggag ggcggtgctc gagacatcca gacgggcggt
      721 cagttcggac agctgcggtt ccggccagtc tgcgccgaac agtcgcacga ttcgtgccgc
      781 gatcagtggg tggctctccc ggcgaagcag ttccggggca agtgccagcg acagtcgccg
      841 ttgttccggg ctcacccgag tcagaatcgt tgcccgttcc tgaggggaaa gggttacgaa
      901 atggtcgggg gaaaggggtt gggcgaccgg ttccgtggtc tcgacggtcg caggagctct
      961 ggccagtctc gctctcacgg tatcgatggc ttgttgcaga aggtcctggc attcttcatc
     1021 ggcctcgacg gtggcgacct tttcaag
//
LOCUS       DAORHD010001739         1047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_214866_length_1047_cov_2.511089, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001739 DAORHD010000000
VERSION     DAORHD010001739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_214866_length_1047_cov_2.511089"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1047
                     /locus_tag="PKO06_24880"
     CDS             <1..>1047
                     /locus_tag="PKO06_24880"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV72968.1"
                     /translation="RWNSRELFMLAVVGLGLGIGYLTHLAGLSLAFGAFVTGLILNES
                     EYGHKAMQEMMPIRDLFSLLFFVSVGMLLDPAVIWQHLYTVVFLTVVVCGGRGLLLAV
                     TAYGFGYRRIIPVAVFFGMLPISEIAFIVIRVGGSAGTLSADIYALILNAVVISMLAG
                     PLAAGLVTPFYAGLRRLWPEETINAVNLPAAPLGNHAIIAGGGKLARYVASALRESGI
                     PYVIIEPLYQSFLDGKALNLVMIYGEPGQDRVLEMAGLERARLVLFTGSDRQDIEEIL
                     ERRSVGKLSFRCLIQTDSGNEPPDLRLQEVDAIISPDLEGGLEMARRSMQTLGMATDV
                     VSQVLERVRRQQHS"
BASE COUNT          175 a          288 c          329 g          255 t
ORIGIN      
        1 cccgctggaa ctcgcgcgag ctgttcatgc tggcggtcgt cgggcttggt ctgggcatcg
       61 gttacctgac gcatctcgct ggtctttcgc tggcattcgg cgcgttcgtc accggcctga
      121 tcttgaatga gtccgagtat gggcacaagg cgatgcagga aatgatgccg attcgagatc
      181 tgttcagtct gctgtttttc gtgtcggtcg gcatgctgct tgacccggct gttatctggc
      241 aacacctgta caccgtggtc tttctgacag tggtggtctg cggcggtcgc ggtctgcttc
      301 tggccgtcac cgcctacgga ttcggctatc gacgtatcat tccggttgcc gtgttcttcg
      361 ggatgctacc gatttctgaa attgccttca tcgtgattcg cgtcgggggc agtgcgggta
      421 ccctgtcggc cgatatctat gctctgattc tgaacgccgt cgtgatctcg atgcttgccg
      481 gacctctggc cgctggtctg gtcaccccct tctatgctgg gttgcgtcgc ctctggccgg
      541 aagaaacgat caatgcggtg aacctgccgg cggcgccgct ggggaatcat gcgatcatcg
      601 ccggcggcgg gaaactggcg cgttacgtgg cgtcggctct gcgtgagagc ggtattccct
      661 acgtcatcat cgagccgctc tatcaatcct ttcttgatgg aaaagccctc aatctggtga
      721 tgatctatgg cgaacccgga caagatcggg tgctggaaat ggccggtctt gaacgtgcgc
      781 gactggtgct cttcaccgga tctgaccgcc aggacattga agagattctt gagcggcgga
      841 gcgtcggcaa actttcgttt cgatgcctga ttcagaccga ttccggcaac gaaccaccgg
      901 atctgcgcct gcaggaggtc gatgccatca tctcgccgga cctggaaggg gggctggaaa
      961 tggcgcgcag aagtatgcag acgctcggta tggcgactga cgttgtatcg caggtgctcg
     1021 agcgagtcag aaggcagcag cattcag
//
LOCUS       DAORHD010001740         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_215090_length_1046_cov_4.500505, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001740 DAORHD010000000
VERSION     DAORHD010001740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215090_length_1046_cov_4.500505"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PKO06_24885"
     CDS             <1..204
                     /locus_tag="PKO06_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72969.1"
                     /translation="DLTDKPDQRVLPGGAASPGPALVTPAPASLQGANRQPIPLRREP
                     RPVSLNNLCSCSSILFDDCMESF"
     gene            329..925
                     /locus_tag="PKO06_24890"
     CDS             329..925
                     /locus_tag="PKO06_24890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV72970.1"
                     /translation="MMRRFALLLALLCAALPAFAQDAAPVAAPAAAPVAAPAAEPAPA
                     VEKTPATPTSSAWNTFFPDGLINSAGEKVELSVLDNKIVGLYFSAHWCPPCRAFSPKL
                     VEFRNKNKDQFEVVFISSDKNETAQFEYMKEVGMEWPTMKHRSEAANALAEKFGVQGI
                     PTLIILSPTGETVSTSGRMDVTDNPDGCIAKWKEGPAK"
BASE COUNT          194 a          328 c          289 g          235 t
ORIGIN      
        1 gacctgacag acaaacccga tcagcgggtt cttcccggcg gagcggcctc gccaggaccg
       61 gcgctggtaa cgcctgctcc cgcgagcctt cagggcgcga accggcagcc cataccgctg
      121 cggcgcgagc caagaccggt gtcgctgaac aatctctgct cctgttcatc cattctcttc
      181 gatgactgca tggaatcatt ctgatttcct ctcttttccc gtctcggcct gccgatccgc
      241 tttcggcagg ctttttttct tggcgggcga atgtgctatc ataccgctat cgttttgatc
      301 gaatatcact tctggaggct caacacgtat gatgcgtcgt ttcgctctgc tgctcgctct
      361 gctttgtgcc gctctgccgg cttttgccca ggatgccgct cccgtcgcgg cacccgcagc
      421 tgctccggtt gccgctccag ccgcggaacc ggctccggca gtcgagaaga ctccggccac
      481 gccgaccagt tccgcctgga acacgttctt ccctgacggc ctgatcaaca gcgccggcga
      541 gaaagttgaa ctgagcgtgc tcgacaacaa gatcgtcggg ttgtactttt ccgctcactg
      601 gtgccccccc tgccgcgcgt tttcgccgaa gctggtcgag ttccgcaaca agaacaaaga
      661 tcagttcgag gtcgtgttca tcagctcaga caagaatgaa accgcccagt ttgagtacat
      721 gaaagaagtc ggcatggaat ggcccacgat gaagcatcgc tctgaagcgg cgaacgccct
      781 ggccgagaaa ttcggcgtgc agggaattcc gacgctgatc atcctctcgc ccaccggtga
      841 aaccgtttct accagcggcc gcatggatgt caccgacaat ccggatggct gcatcgccaa
      901 atggaaagaa ggccctgcca agtaacgctc tggctccgaa aaaaacaccc cgactctggt
      961 gagccggggt gttttttttc tgcgaaaacg ttcagtggtc gcgtgctttt tcgtcttttg
     1021 cggggggagc cgctggggtt tcggtc
//
LOCUS       DAORHD010001741         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_215494_length_1045_cov_3.740404, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001741 DAORHD010000000
VERSION     DAORHD010001741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215494_length_1045_cov_3.740404"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PKO06_24895"
     CDS             complement(<1..463)
                     /locus_tag="PKO06_24895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72971.1"
                     /translation="MSMKRVLVAAVVLLFWLSMAVAQAQSSSTGYVPSYKIGDKWFVE
                     ATYRDMKTPGEPWMTPIVWTFDVKNIKPIAGQNAYVIHVYPKNRGLKMQAILYLATND
                     LRPLKVIDIFPRFLGVKHQVRDLDPNTPEPLLSENTLVPYDLPVFPLQKASV"
     gene            complement(713..>1045)
                     /locus_tag="PKO06_24900"
     CDS             complement(713..>1045)
                     /locus_tag="PKO06_24900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV72972.1"
                     /translation="RVLEMVGFEVVGVSDAREALKFFEAEPAEYALVLTDLTMPGMTG
                     LEMARSMRQTRQDIPIILWTGYSDKCYLEDSNATPESLGVDDLLNKPVSIEQLIRAVK
                     NTLSRRKT"
BASE COUNT          241 a          304 c          280 g          220 t
ORIGIN      
        1 gcaccgaagc cttctgcagc ggaaacaccg gcagatcata gggaaccagc gtgttttcgc
       61 tcagcaacgg ctcaggagtg ttcgggtcga gatcgcgcac ctgatgtttc actccaagga
      121 aacgcggaaa aatgtcgatc actttcagtg ggcgcaggtc gttggtcgcc agatacagaa
      181 tcgcctgcat cttcagccca cgatttttcg ggtacacatg aatgacatag gcattctgcc
      241 cggcgatcgg cttgatgttt ttcacatcga acgtccagac gattggtgtc atccagggtt
      301 cgcccggggt cttcatatcg cggtaggtgg cctcgacaaa ccacttgtca ccgattttgt
      361 aggacggaac gtacccagtc gaagagcttt gggcttgagc caccgccatc gacagccaaa
      421 acagcagcac cacagccgca accagcacgc gtttcatcga catcacccac ctcctgccac
      481 aaaaagtgcg cattgacaga cctttttcat tgtaccctct gtttggaaaa gcgtcaacca
      541 gcaggcgcag ccatgggaaa tgcacacttt tcctgcgtgg gctcgggctt ttcgactcac
      601 ccgcgtgcag agcagaggga ccgcatcaca ggagcctgca gcgacagggt cgcaggatcc
      661 ttcgggggaa aagaggaaaa aagcgggcat cgaccggctc tgtcgaacga gctcaggtct
      721 ttcgtctact caaagtattc ttcacggccc tgatgagttg ttcgatggaa acgggcttgt
      781 tcagaaggtc atcgaccccc agggactccg gagtggcatt cgagtcttcc agatagcatt
      841 tgtcgctgta acccgtccac aggatgatgg gaatgtcctg gcgcgtctgg cgcatactcc
      901 gcgccatttc gagaccggtc atgccgggca tggtcaaatc tgtcaacacc agagcatact
      961 cggctggctc tgcctcaaag aacttcagcg cttcgcgcgc gtcactgacg ccgacgactt
     1021 cgaaaccaac catttcgagc acacg
//
LOCUS       DAORHD010001742         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_215552_length_1045_cov_2.854545, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001742 DAORHD010000000
VERSION     DAORHD010001742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215552_length_1045_cov_2.854545"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1045)
                     /gene="fliD"
                     /locus_tag="PKO06_24905"
     CDS             complement(<1..>1045)
                     /gene="fliD"
                     /locus_tag="PKO06_24905"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNV72973.1"
                     /translation="IGSSNDTSNFLSVTGLVAGSQATSAAKESSASVGAITANQANTV
                     TLSALPLVPAVGAGTNYLRVTVNGVATDVAYQNTDTLNKVLDEIRKVEGIEDAYYDET
                     TQKVKIVTSSTGTSASLKIEDVAGGTLGAGLKISADVATGTLLGDTMVGGRPLSAIST
                     TKNMGAGGFATPITSGNFTINGVSFLVSDPNSVSLQTIIDTINNNTRVGVTAKYDESS
                     GSLILTSKGTGNTSISLGASTDTSNFLSAVGLMNTPQQIGQNAIYRVKGMFGDQDQVS
                     QSNEIRTIATGLTVTLKSETDAAGETVNVQADTSVARKAIDDFITSYNETMTMVYTKL
                     SEKRDYTLEALSET"
BASE COUNT          205 a          319 c          269 g          252 t
ORIGIN      
        1 gtttcactga gcgcttcgag tgtgtagtca cgcttctcag agagtttggt atacaccatc
       61 gtcatcgtct cgttatacga cgtgatgaaa tcatcgatgg ctttgcgcgc gacactggtg
      121 tcggcctgca cgttcacggt ttcaccggcg gcatcagtct cgctcttgag ggtcacggtc
      181 aaccccgtcg cgatggtcct gatctcattc gactgactga cctggtcctg gtccccgaac
      241 atccccttca cgcgatagat cgcattctgg ccgatctgct gcggcgtatt catcaacccg
      301 accgccgaca ggaaattgct cgtgtctgtc gatgccccga ggctgatact ggtgttgccg
      361 gttcctttcg aggtcaggat cagcgagccc gagctctcat catattttgc cgtgacgcca
      421 acccgcgtgt tgttattgat cgtatcgatg atcgtctgaa gtgagacaga attgggatcc
      481 gatacaagaa aactcacacc gttgatggtg aagtttccgg acgtaatggg cgtggcaaat
      541 cccccggctc ccatgttctt cgtggtggaa atcgccgaca gcggccgtcc tccaaccatc
      601 gtatcgccaa gcagcgttcc ggtggctaca tcggcactga tcttcaaccc agctcccagc
      661 gttcctcccg ccacatcctc gatcttcagc gaagctgaag tcccagtcga ggaggtgacg
      721 atcttcacct tctgagtcgt ctcatcgtag taggcgtctt cgatgccttc aaccttgcga
      781 atctcatcca gaaccttgtt cagggtatca gtgttctgat aggccacatc cgtcgcgaca
      841 ccattgaccg tcacccgcag atagttcgtc ccggcgccaa ctgccggaac caggggcaat
      901 gcggacagag tgaccgtatt cgcctggttc gcggtaatcg ccccaacgct ggcggaactc
      961 tctttcgccg ctgacgtcgc ttgactaccg gcaaccaatc cggtcaccga caggaagttc
     1021 gacgtatcgt ttgacgaccc gatac
//
LOCUS       DAORHD010001743         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_216132_length_1044_cov_1.498483, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001743 DAORHD010000000
VERSION     DAORHD010001743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216132_length_1044_cov_1.498483"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1044
                     /locus_tag="PKO06_24910"
     CDS             <1..>1044
                     /locus_tag="PKO06_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008697349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV72974.1"
                     /translation="CMASAVTGFLATYGVDEPAGCYDDLDACDVLIMWGNNPAEMHPV
                     LFSRVIDRRSRGEKVTLIDIGTRRTRTTEACQHYLEMKGHGDLAIANGIAHLLIKNGT
                     YDQKFVESFCSFKAPDDANPNLHGKVISFAEYKTLLEPYTPEHVEQLSGVPASQIRML
                     ADLFGNRKLRITSLWCMGMNQHTRGTAINTMVHGIHLLSGHFGTPGNAPTSLTGQPSA
                     CGTVREVGTLSHALPGGLVVTDAENRTKAEEIWQVPPGRINSVPGHHTVKMWESFCTP
                     TSEGGDIGTIWVQVTNPGQTLPNLNKLFNPKGDSKAGLEDKFLIVSDVYPTATTRLAD
                     LILPSAMWVEKNGM"
BASE COUNT          254 a          275 c          291 g          224 t
ORIGIN      
        1 tgcatggctt cagcggttac aggatttctg gcaacgtatg gcgttgatga gcccgcaggc
       61 tgttatgacg acctggacgc atgtgatgtg ttgatcatgt ggggcaataa cccggccgaa
      121 atgcatccag tgcttttctc acgagtcatc gaccggcgca gtcgcgggga aaaggtgacg
      181 ttgatcgata ttggcactcg gcgaactcga accacagaag catgccagca ttacctcgaa
      241 atgaaagggc acggcgacct tgcgatcgcc aacgggattg ctcacctttt gatcaagaac
      301 ggcacctatg atcagaagtt tgtggagtca ttctgcagct tcaaggctcc ggatgatgcc
      361 aatccgaatt tgcacggcaa ggtcatctcg ttcgccgaat acaagacact gctcgagcct
      421 tacacgcctg agcacgtcga gcagctgtct ggcgttcctg cctctcagat tcggatgctg
      481 gcagatttat ttggcaaccg gaaactcagg atcacatcgc tgtggtgtat ggggatgaac
      541 cagcacactc gcggcactgc gatcaatacg atggttcacg gcatccattt gctcagcgga
      601 cattttggaa cacccggcaa tgcaccgaca agtctgaccg ggcaaccatc ggcctgcggg
      661 acagttcgag aagtgggaac tctttcgcac gcgctgcctg gtggcctcgt tgttactgac
      721 gctgagaatc gaaccaaagc agaggaaatt tggcaggttc ctccggggcg aatcaactca
      781 gttcccggac atcacaccgt aaaaatgtgg gagagtttct gtactccgac aagcgaaggc
      841 ggggacatcg gaactatctg ggttcaggtc acgaatccag gacaaactct gcccaacctg
      901 aacaagctgt tcaacccgaa gggagacagc aaggcaggtc tggaagacaa gttcctgatt
      961 gtcagcgacg tctatcccac cgcgacaacg cgactggccg atttgattct gccttctgcc
     1021 atgtgggttg aaaagaacgg catg
//
LOCUS       DAORHD010001744         1042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_216635_length_1042_cov_3.223911, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001744 DAORHD010000000
VERSION     DAORHD010001744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_216635_length_1042_cov_3.223911"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKO06_24915"
     CDS             complement(<1..419)
                     /locus_tag="PKO06_24915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV72975.1"
                     /translation="MPVPLFYTAPVNTTGGLLWSCNFAVKRSVFILVGGFDENFRFAH
                     LEDVDLRIRLQQHGYRMAFVPQAVVHHPPRPFDDDFSIPWLYESEFYFRSKHRISIAK
                     AGLNLRNIFGFHARLIKNSRSCSDLFRILRRFLFKYG"
BASE COUNT          281 a          240 c          282 g          239 t
ORIGIN      
        1 catccgtatt tgaaaagaaa gcgccgcaga attcgaaaaa gatcggaaca acttcgagaa
       61 ttcttgatga gcctcgcgtg aaaaccgaaa atgtttctca ggttgagacc ggctttcgca
      121 attgagattc gatgttttga tcgaaagtag aattcagatt catagagcca gggaatggaa
      181 aagtcatcgt cgaatggtcg gggaggatga tgaacaacag cctgaggcac gaaggccatg
      241 cgatatccgt gctgctgcag gcgaatgcga agatcaacat cttcgaggtg agcaaaacgg
      301 aagttttcgt caaagccgcc aacaagaata aacaccgatc gcttcacggc aaagttacag
      361 ctccacaata gaccacccgt ggtgttcacc ggtgcagtat aaaaaagtgg aacgggcacg
      421 tccgtttcgg ttttcccctc cagtactggt tccgactctc gcgatgcccg atcgtatgcg
      481 gaaagccacg tggatgaagg caggcaatcg tcatcgataa ataccagcca ttgtccacgc
      541 gcgtgatgag cgccacagtt gcgattcgac gccgggcctc ttcgtggtcc ctgaatccag
      601 cgcacgcttg gaaatctccg actgatcaaa tgctgcgttt gaaagtctcg gctgtcgtcg
      661 gaaacaatga tctcgtattg ctctcgcctg aagtcctgct gatgaaggag tttttccaga
      721 cagtgactca aaagctctgg tcgattgcag gtgggaacga tgacggagaa atggattgaa
      781 tctatgagag attggaccat tcgagtaaaa aggccttcaa tcctttcttg actgatccga
      841 tcagacgctg gaagagcggg agtcgctttg ggggagggaa tcggctcaaa aatagctgaa
      901 gctcagcgtc agtgaaatac tgaaacatga gacgatctat gggatgatct cctgtaggtt
      961 tttgtcccgc ccaatggatg agaagcggct ttcgagcttc atcatgccag tagtgggaat
     1021 actcgcctgg gtagtctcct gc
//
LOCUS       DAORHD010001745         1040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_217373_length_1040_cov_3.475127, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001745 DAORHD010000000
VERSION     DAORHD010001745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217373_length_1040_cov_3.475127"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..>1040
                     /locus_tag="PKO06_24920"
     CDS             155..>1040
                     /locus_tag="PKO06_24920"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNV72976.1"
                     /translation="MRLKEILVRVPKETGVREKLYLSGNHSALGRWQLPGIPLERIGE
                     GLYLARVAVPDGQTIECKVHRGNWKKVEKDAAGGDILNHRLIMKPGRRQEIRVARWKP
                     RDKPANSTALESVRYHRRFVSKHLGNSRSLAVYLPPQYESEPQRRFPVLYMHDGQNLF
                     DVATAFAGVEWGADKTAQRLIEAGRLEPLIIVGIYNNEDRLNEYAPISELSRQAGGRG
                     ADYARFVVEEVKPFIDHTYRTLPQPEHTGVAGSSLGGLISLYLGLEYPHVFSRLGVIS
                     PALWWGRQWILSQARRKGR"
BASE COUNT          210 a          285 c          332 g          213 t
ORIGIN      
        1 ccgtcgacgc ccatgtatcg ccccaataac tgaacgcaac tgatcgacgc gccgacctgc
       61 gggtcggcgt tttctttttc cggcatttca ccagcgccga gaatgtggta caagaaggcg
      121 cacaattcaa gccacggttc gaggaggttt gcgcatgcgt ctgaaggaaa ttctggtgcg
      181 ggtgccgaag gaaaccggag tccgcgagaa gctgtacctg tccggaaatc attccgcgct
      241 gggccgctgg cagctgcccg gtattccatt ggaacgcatc ggggaagggc tgtatctcgc
      301 tcgagtggcc gttccggatg gtcagacgat cgagtgcaag gtgcatcgtg ggaactggaa
      361 gaaggtggaa aaggatgccg caggcggcga tattctgaat caccgcctga tcatgaaacc
      421 tggtcgtcgc caggagattc gggtggctcg ttggaaaccg cgcgacaaac cggcgaactc
      481 cacggctctc gagagtgttc gctatcatcg gcgtttcgta tcgaaacatc tggggaactc
      541 tcgttctctg gcggtgtacc tgccaccgca gtatgaaagt gagccacaga gacgattccc
      601 ggtgctgtat atgcacgatg gccagaatct gttcgatgtg gcgactgcct ttgccggggt
      661 cgaatggggg gccgacaaga ccgcgcagcg attgatcgag gccggacgtc tcgagccgct
      721 gatcatcgtc ggcatctaca ataacgagga ccgtctgaat gagtatgcgc ccatttctga
      781 actgagtcga caggcggggg gcaggggggc cgactacgca cgcttcgtcg tcgaagaggt
      841 caagccattc atcgatcaca cctatcgtac tctgccacag cccgaacaca ccggcgtggc
      901 cggttcgtcg ctcggggggc tgatctcgct ctacctgggg ctcgagtatc cgcacgtgtt
      961 ttcacgtctg ggagtcatct cgccggctct gtggtgggga cggcagtgga ttctgtcaca
     1021 ggcgagacgt aagggccgct
//
LOCUS       DAORHD010001746         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_218556_length_1037_cov_2.977597, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001746 DAORHD010000000
VERSION     DAORHD010001746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218556_length_1037_cov_2.977597"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..>1037)
                     /locus_tag="PKO06_24925"
     CDS             complement(61..>1037)
                     /locus_tag="PKO06_24925"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV72977.1"
                     /translation="DPGLPELRQTLSAVLRQRLSHADLTPENLLITAGANSAYLMTVM
                     GIMRSGDRMGLLTPWYFNHEMSVRLLGHETIPIALSSAQAFTLDTERILDAVDRYKLQ
                     ALTLVNPNNPTGTTYAPEHLTHLALELQRRGVWLIADETYAFFSGTDHRPFSLGSLAD
                     RLDRLVVIGSFSKTFAMTGWRVGYLAARATLIRELLKIQDTMLICASHPGQRLALICL
                     RDGWNWLAERILDLEQRQRQFIAEWPIDCPWRIVSSGLFFAWLEGPTAGRELVLRLLE
                     TAGVILIPGDIFGPGLDRTIRVSLGCVDDQGFAEGKQRLFRSLQTSVS"
BASE COUNT          251 a          280 c          318 g          188 t
ORIGIN      
        1 caaggatcat cagaaacaga ccgattttca acccttgcga aaatgacatg aagtgctccc
       61 tcaagacacg gatgtctgca gactgcgaaa caggcgctgt tttccctcgg caaaaccttg
      121 atcatcaacg catccgagtg aaacacgaat agtacggtca agccccgggc cgaaaatatc
      181 acccgggatc agaatcacac cggcggtctc gagtaatcgc aggaccagtt cgcgtcccgc
      241 ggttgggcct tccagccagg caaagaagag accggaagag acgattcgcc agggacagtc
      301 gatcggccac tcggcgataa actgccgctg acgttgctcg agatccaaga tgcgctcggc
      361 aagccagttc cagccatcgc gcaggcagat gagggccagg cgttgaccgg ggtgggaggc
      421 gcagatgagc atggtatcct gaatcttcaa caactcgcga atcagggtgg cgcgtgccgc
      481 cagatagccg actcgccacc ccgtcatggc gaaggtcttc gaaaaactgc cgatcacgac
      541 aagtcggtcg agtcggtcag cgagtgagcc cagcgaaaat ggtcgatgat cggttcctga
      601 aaaaaaggcg taggtctcat cggcgatcaa ccagaccccg cgtcgctgca gttcaagtgc
      661 cagatgtgtc aggtgttccg gggcataggt cgtgccggtg ggattattgg gattgacgag
      721 agtcagggct tgaagtttgt accgatcgac agcgtcgaga atccgctcgg tatcaagagt
      781 gaatgcctgt gcggatgaaa gcgctatcgg gatcgtctcg tggccgagca ggcgcaccga
      841 catctcatga ttaaaatacc agggggtgag aagtcccatc cgatcgcctg accgcatgat
      901 tcccatgacc gtcatcaggt aggcggaatt cgcgccggca gtgatcagca ggttttccgg
      961 ggtcaggtcg gcatgcgaca ggcgttggcg tagaacggca gaaagcgtct ggcgcagttc
     1021 cggcagcccg ggatcga
//
LOCUS       DAORHD010001747         1034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_219677_length_1034_cov_3.673136, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001747 DAORHD010000000
VERSION     DAORHD010001747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_219677_length_1034_cov_3.673136"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..>1034
                     /locus_tag="PKO06_24930"
     CDS             40..>1034
                     /locus_tag="PKO06_24930"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV72978.1"
                     /translation="MRRYIDVLEDYIAQLENEVLKDSHNSSRPPSSDRYPIKREKKTS
                     GRFPGGQPGHMGVTRTLQVADTTIQYAPETCIHCGVSFLEDNPVIGKPMIRQQAELAE
                     KPVLIIQREYHQRKCPCCGRLSRATPAPGEEQWLGPRLKALANFLTTECHVSNSKATA
                     FFSEGLGVTAATGTWFKTRAALSEALTYSTRTVFQTVQVASEKNVDETSWKQGAHREY
                     LWTISTANATFYAILPTRGGIAAQSLLGEKPIGNITTDRFVVYDFIPPAQHAYCWSHL
                     DRDFQWFAEREDSERKDFGKQGLRAVDALFTAWRRWRDGETTEWEYEDAMNAVRR"
BASE COUNT          262 a          261 c          283 g          228 t
ORIGIN      
        1 aaggttcctc gagaagacct ggagaaaaca cctcccaccg tgaggcggta tatcgacgtt
       61 ctcgaggatt acatcgctca gttggaaaac gaggtcttga aagattcaca caattcatca
      121 cgacctccct cgagcgatcg atatccgatc aagcgagaaa agaaaaccag cggtcgtttc
      181 ccgggtgggc aacctggaca catgggagtc actcggacat tgcaggttgc tgacacgacc
      241 attcaatatg ctccagaaac ctgtatccat tgcggagtgt cgttccttga agataacccc
      301 gtcattggca agccgatgat ccgccagcag gcggaattgg ccgaaaagcc tgtgttgatc
      361 attcaaaggg aataccatca gcggaaatgc ccgtgctgcg gtcgcttgtc tcgtgcaact
      421 cctgcgcccg gagaagaaca gtggcttggt ccaagactga aggctctggc caactttctg
      481 accacggagt gtcatgtcag taactccaag gcgacggcgt ttttcagcga aggtcttgga
      541 gtaacggccg ctactggaac atggttcaag acacgtgcag ccctttctga agctctgacg
      601 tattccacca gaacggtgtt tcaaaccgtc caggttgcat cggagaagaa tgttgatgaa
      661 acttcgtgga agcagggggc tcatcgagaa tatctctgga cgatcagtac ggcaaacgcg
      721 accttttatg ccattcttcc cactcgtggc ggtattgccg ctcaaagcct gcttggggaa
      781 aagcccatcg ggaacattac gactgatcga ttcgttgtct atgacttcat tccgccagcc
      841 cagcatgcct attgttggtc tcacctggac cgagactttc agtggtttgc agaacgggaa
      901 gacagcgaga gaaaagactt tggaaaacaa ggtttgagag ctgttgatgc cctcttcacg
      961 gcttggcgcc gctggcgaga cggggaaacg acggaatggg aatacgaaga tgcgatgaat
     1021 gccgttcgtc gaag
//
LOCUS       DAORHD010001748         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_220154_length_1033_cov_2.343558, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001748 DAORHD010000000
VERSION     DAORHD010001748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220154_length_1033_cov_2.343558"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKO06_24935"
     CDS             <1..283
                     /locus_tag="PKO06_24935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72979.1"
                     /translation="TGCITCRKSGYISRTAIHEIMTINDEIHSLVINNASARIIRTAA
                     VHSGMRTMREDGLRKMLRGVTTYDEVIRLTRRDDVEARETASSRQAPAA"
     gene            280..>1033
                     /locus_tag="PKO06_24940"
     CDS             280..>1033
                     /locus_tag="PKO06_24940"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV72980.1"
                     /translation="MSKRLFQYRAIDSRGKTMEGSMEAQSPEEVGTWLTERQYYVVDI
                     HQSTLGTLASIPRIRSIPRPELNYFLIQLSSLIEAGCPLLLSLQALHRQLSPGPLKSL
                     VKDLKEKIEVGKSFSEALKSYPDVFSQLFITLVEVGEIGGILSEVLDRYAKIHDAMYR
                     IRSKVIKSMIYPALLLTLTIVFTWVLLVWVFPTFIEKFQEGGIPLPLPTHILLTVSNF
                     LRHNWLALGFGVVALWQAFRLFRQSKPGGQFLG"
BASE COUNT          212 a          311 c          275 g          235 t
ORIGIN      
        1 caccggctgc atcacctgtc gcaagagcgg ttacatcagc cgaaccgcta tccacgagat
       61 catgacgatc aatgatgaga ttcactccct cgtcatcaac aatgcctcag cccgcatcat
      121 tcgcaccgcc gccgttcact cgggcatgcg caccatgcgc gaagacgggc tgcgcaaaat
      181 gctcaggggt gtcaccacct acgacgaagt gattcggctc acgcggcgcg acgacgtcga
      241 agcccgcgaa acggcttcct ccagacaagc accggcggca tgagcaagcg gctctttcag
      301 tatcgggcca tcgacagccg gggcaagacc atggaaggct ccatggaagc ccagtctcca
      361 gaagaagtcg gcacctggct gactgagcgt cagtattatg ttgtcgatat ccatcaatcg
      421 acactcggca ctctggcctc gatcccgcgg atccgttcga ttcctcgccc ggagcttaac
      481 tactttctga ttcagctctc tagcctcatc gaggctggtt gtccgctgct gctcagtctg
      541 caggctctgc atcgccagtt atcgcccggt ccgttgaagt cgctggtgaa agatctgaaa
      601 gagaagatcg aggtgggcaa atcgttttcc gaagcgctga agtcttatcc agacgtcttt
      661 tcgcaactgt tcattacgct ggttgaggtc ggtgaaatcg gcggcatcct gtcagaagtc
      721 ctcgatcggt atgccaagat ccacgacgcg atgtatcgaa tccggtcgaa ggtcatcaaa
      781 tcgatgatct acccagcgtt gctgctcacc ctgaccattg ttttcacctg ggtgctcctg
      841 gtctgggttt ttccgacctt catcgaaaaa tttcaggaag ggggcattcc tcttcctctg
      901 ccgacgcaca tcctgttgac cgtatcaaac tttctgcgcc acaactggct ggccctgggc
      961 ttcggcgtgg tggcactctg gcaggcattt cggctgttcc ggcagtcgaa gccaggaggc
     1021 cagtttctcg gtg
//
LOCUS       DAORHD010001749         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_220460_length_1032_cov_2.794268, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001749 DAORHD010000000
VERSION     DAORHD010001749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220460_length_1032_cov_2.794268"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKO06_24945"
     CDS             complement(<1..556)
                     /locus_tag="PKO06_24945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV72981.1"
                     /translation="MSSQTATDQRITTRSPRSLNGIRLLLALGTLCLLYYHLGTLALT
                     ESSEARYGSIARTMLDTGDWINPVMNGLPHYSKPPLAYWSSALGMKLCGINEFGARFF
                     LPIAGVLTVLGCFEIGLLLGSLRLALCSSLALVTSLMFLVLFRGLTADPWLAAWETWM
                     IWALLAHDRRPESRYAIGFWFTVSP"
     gene            complement(534..818)
                     /locus_tag="PKO06_24950"
     CDS             complement(534..818)
                     /locus_tag="PKO06_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HNV72982.1"
                     /translation="MSARFWLIFGLVGQTMFFMRFLVQWIVSEREKRSVIPVSFWYFS
                     LLGSAILAVYAFHIQDPVFILGQSLGFVIYIRNLMLIKNQQETNELSNGN"
     gene            complement(808..>1032)
                     /locus_tag="PKO06_24955"
     CDS             complement(808..>1032)
                     /locus_tag="PKO06_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV72983.1"
                     /translation="LRRIPLFEGMHRFFPALVKLEGGSVIEVKVSHHPRTQGVSKYNV
                     LNRVFRSFLDLLAFAWMRWRHLGYKVTHEC"
BASE COUNT          270 a          288 c          307 g          167 t
ORIGIN      
        1 tgggggatac cgtgaaccag aacccgatgg cataacgcga ttcaggacgg cgatcatgcg
       61 ccagcagggc ccagatcatc caggtttccc aggccgccag ccacgggtcg gcggtcaacc
      121 cgcgaaacag caccagaaac atcaggctgg tgaccaatgc caggctcgaa cagagcgcca
      181 ggcgcaaact gccgagcagc aacccgatct cgaaacaccc gagtacggtc agaaccccgg
      241 cgattggcag gaaaaaacgg gcgccgaatt cgttgatccc acagagtttc atgcccagcg
      301 ccgaagacca gtaggccagc gggggcttcg agtaatgcgg cagtccgttc atcaccgggt
      361 tgatccagtc acccgtgtcg agcatggtgc gggcgatcga accatacctc gcttctgagc
      421 tttccgtcag cgccagcgtg cccagatggt agtacaacag acagagcgtg ccgagagcca
      481 gaagcaggcg aataccgttg agagagcgag gagagcgggt agtgatcctc tgatcagttg
      541 ccgtttgaga gctcattggt ttcctgttga tttttgatga gcatgagatt gcggatgtag
      601 atcacgaaac cgaggctctg gccgagaatg aagacgggat cctgaatgtg aaaggcgtag
      661 acggccagaa ttgccgaacc cagaagactg aaataccaga aagaaacggg aatgacgctg
      721 cgtttctcgc gttccgaaac gatccattgc acgagaaagc gcatgaagaa catggtctgt
      781 ccgaccagac cgaagatcag ccagaatcta gcactcatga gtcaccttgt agccgagatg
      841 gcgccagcgc atccacgcga acgcgagcag atcgagaaaa gaccggaaca cccggttcaa
      901 cacgttgtat ttggaaacgc cctgggtgcg cggatgatgg gaaaccttca cctcgatgac
      961 cgagccgccc tcgagcttga ccagcgccgg aaagaaccgg tgcatgccct cgaacagcgg
     1021 aatccgccgg ag
//
LOCUS       DAORHD010001750         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_220782_length_1031_cov_4.031762, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001750 DAORHD010000000
VERSION     DAORHD010001750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220782_length_1031_cov_4.031762"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PKO06_24960"
     CDS             complement(<1..185)
                     /locus_tag="PKO06_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72984.1"
                     /translation="MHLAFFLAALALVTHFVLGCLVLVRAPSNPAHRTFSGMLFLFFV
                     WSAAELLLLTYPTRSVFR"
     gene            complement(193..813)
                     /locus_tag="PKO06_24965"
     CDS             complement(193..813)
                     /locus_tag="PKO06_24965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV72985.1"
                     /translation="MRRRAAFTLVELLIGMALTILMGGTLYLFQSQGLSTVTKGTIRL
                     NLQSEIRRKLEKLVIDLRACNEVLEIQPDMIKFTRFRSSTEESDAGEINLDTITYTLA
                     KVSNRWSLLRAENNQQPLELISHDHIEGNLFFPLYEDTGDPGADTPPQLLPFDMNAND
                     SGQRKRITFIRLRIRVRQAREFVGFTTAVTLRAPYQRLQQPHWKFR"
     gene            complement(810..>1031)
                     /locus_tag="PKO06_24970"
     CDS             complement(810..>1031)
                     /locus_tag="PKO06_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72986.1"
                     /translation="KEIATRLMKKYETAFRRPYEIYPDEAGSFRRCLIVDDKYDRKSP
                     PRLKKVTVRVLDKRGHRMAEVVTLVGRHE"
BASE COUNT          220 a          277 c          311 g          223 t
ORIGIN      
        1 cgaaagacgc ttcgtgtggg atacgtcagc agcagtaact cggcggccga ccagacgaag
       61 aaaagaaaca acatgccgct gaaggtgcgg tgagccggat tgctgggggc tcgcacgaga
      121 accagacacc ccaggacaaa gtgcgtgacc agggccagag cggcaagaaa aaaggcgaga
      181 tgcatggttc cgctagcgga acttccagtg cggttgctgc aacctctggt agggagcgcg
      241 cagtgtcacc gcggtggtaa agcccacaaa ctcgcgagcc tgacgcaccc ggatgcgcag
      301 acgaatgaat gtaatgcgct tgcgttgtcc cgaatcgttg gcattcatgt cgaagggcag
      361 gagttgcggt ggcgtgtccg cgcccggatc gcccgtgtct tcatacaggg gaaagaagag
      421 gttgccctcg atatggtcgt ggctgatcag ttccagaggc tgctgattgt tttctgcccg
      481 cagcagagac cagcggttgc tgactttggc gagggtgtag gtgatggtgt cgaggttgat
      541 ctctccggcg tcgctctctt cggtggatga acgaaaccgg gtgaacttga tcatgtccgg
      601 ttgaatctcg aggacttcgt tacaggcacg cagatcaatt accagtttct cgagttttcg
      661 tctgatttcc gactgcaggt tcagacggat cgtccccttg gtaacggtgc tcaggccctg
      721 actctgaaac agatacagcg tgccccccat caggatcgtg agcgccatcc cgatcaggag
      781 ttctaccaga gtgaatgccg cacgtcgtct cattcgtgtc ttcctaccag ggtgacgacc
      841 tcggccatgc gatgaccgcg tttgtccagg acgcgaaccg tgactttctt gagccggggc
      901 ggactcttgc ggtcatattt gtcatccaca atcagacagc gacgaaacga ccccgcctcg
      961 tcaggataga tttcatacgg ccgccggaac gcggtttcat actttttcat caagcgcgtg
     1021 gcgatctctt t
//
LOCUS       DAORHD010001751         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_220880_length_1031_cov_2.734631, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001751 DAORHD010000000
VERSION     DAORHD010001751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220880_length_1031_cov_2.734631"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PKO06_24975"
     CDS             complement(<1..229)
                     /locus_tag="PKO06_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNV72987.1"
                     /translation="MNEFHTLQDVLFVGDTMKMTVDGKVHYFKLKEVSLTLLHASEAE
                     RTRFEISPSGYGIHWPLLDEDLSIDGLLGIVH"
     gene            complement(232..483)
                     /locus_tag="PKO06_24980"
     CDS             complement(232..483)
                     /locus_tag="PKO06_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNV72988.1"
                     /translation="MPTVIYLFGWRFFFYVNEGNEPIHIHCKNAEKEAKFWLDTENFG
                     VEEAYSINFSPRDKRLVKKIIFNHFEYIEERWLELQRRK"
     gene            complement(532..750)
                     /locus_tag="PKO06_24985"
                     /pseudo
     CDS             complement(532..750)
                     /locus_tag="PKO06_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002801971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
     gene            complement(751..>1031)
                     /locus_tag="PKO06_24990"
     CDS             complement(751..>1031)
                     /locus_tag="PKO06_24990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72989.1"
                     /translation="GVPRDGKEPVLEGDALKAFAPRVFESIQLKGFVRGQNGVWSGVF
                     QAPDGRPLILSPGMTREGITLVSTDGKSAQIRYGALLRDLTLNKPAEK"
BASE COUNT          245 a          290 c          261 g          235 t
ORIGIN      
        1 cgtgaacaat acccagcaag ccatcaatag agagatcttc atccaacaac ggccaatgaa
       61 ttccataccc tgacggcgag atctcaaacc gggtacgctc ggcctcggac gcatgcaata
      121 aagtcagcga aacttctttc agtttgaagt agtgcacctt gccatcgacg gtcatcttca
      181 tggtgtcgcc aacaaacaac acatcctgca gggtatgaaa ctcgttcatg actacttcct
      241 tcgctgtagc tcgagccatc gctcttcgat atactcgaaa tggttgaaga taatcttctt
      301 cacgaggcgc ttgtccctcg gcgagaagtt gattgaatac gcctcttcaa caccgaagtt
      361 ctctgtatca agccagaact tggcttcttt ctcagcgttc ttgcaatgaa tgtgaatagg
      421 ttcgttccct tcattcacgt aaaaaaagaa acgccagcca aacaaataga tgacggtcgg
      481 catgatcttg acccctggat tcggtctcgt ttcgttaaac ggtttcgtca gcatgtaaac
      541 cgtatccgga cagatatcct gctcgttcgg ccaggaaatc gtatcgccta cgaccgctac
      601 ctgacgaaag taatcttcat ctcgaagttc ccggaacacc cccgagtcga gatacgttga
      661 gcaatcaaaa atcccttctt ctccatttgt gaacgtgatg tgaagacgat actgctcgag
      721 cggagtcacg gaggcaacac gcgggttcat ctacttctcc gccggtttgt tcaaggtgag
      781 gtcgcggagg agggcgccgt agcggatctg ggcgctcttg ccgtcggtgg agacgagggt
      841 gatgccctcg cgggtcatgc cgggcgagag gatcagcgga cggccgtcag gtgcctggaa
      901 gaccccggac cagaccccgt tctgaccgcg aacgaagccc ttcagctgaa tgctctcgaa
      961 gacgcgcggc gcaaacgctt tcagtgcgtc gccctcgagc accggctctt ttccgtcgcg
     1021 gggaacaccg g
//
LOCUS       DAORHD010001752         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_221980_length_1028_cov_3.156218, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001752 DAORHD010000000
VERSION     DAORHD010001752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221980_length_1028_cov_3.156218"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKO06_24995"
     CDS             complement(<1..201)
                     /locus_tag="PKO06_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72990.1"
                     /translation="MSILLLVLLILTSVRITTRVSFSPAREKSCYANMRVVLGAVEMY
                     NMDNSIFLEEVGTGTVEWLRSLS"
     gene            complement(378..>1028)
                     /locus_tag="PKO06_25000"
     CDS             complement(378..>1028)
                     /locus_tag="PKO06_25000"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV72991.1"
                     /translation="DGAAVAAWLNQAGITCVLLKYRVPHSGCYWDDKAKKHVTPRVPK
                     ALQDAQRAISLVRSRAATYDLDPKKIGVMGFSAGGNLAVLSSTAFKTRAYEPIDEIDT
                     VSCRPDFAIPVYPGHLTMEHKNLTPKKTAAALLNTDIVISPEIPPTLLIHAKDDPVDP
                     VHYSMVYERELKKAGVSVKLNLYKTGGHAFGVRKSGKDTDRWVDDAVAWLKDIGVL"
BASE COUNT          201 a          323 c          290 g          214 t
ORIGIN      
        1 agagagagaa cgcagccact cgacggtgcc cgtgcctact tcttccagaa aaatcgagtt
       61 gtccatgttg tacatctcga cggcgcccag aacgactcgc atattggcgt aacaggactt
      121 ttcccgcgcg ggactgaagc ttacccgagt cgtgatgcga acggaggtga gaatcaggag
      181 cacgagcagc agaatactca cgcctcctat gatcacgtct ctgactttca agccactcct
      241 cctgaactca cgaactcaca gagccaaacc cacttcggcg accttcatgc agacgtcatc
      301 cgttgctgat cttaccaagg ccgacgttct gaatcaacgg ctgtctttgc ctctcccggt
      361 cgaagcggca ggaacgctca cagcacgccg atgtctttga gccaggcgac ggcgtcgtcg
      421 acccagcggt cggtgtcttt gcccgatttg cggacgccga acgcgtgacc gccggttttg
      481 tacaagttca gtttcaccga aacgccggcc ttcttcagtt cccgctcgta caccatcgaa
      541 tagtgaaccg ggtcgaccgg gtcgtccttt gcgtgaatca gcagggtcgg cggaatctcg
      601 ggcgagatga cgatatcggt gttcagcagt gctgcggccg tcttcttcgg cgtcaggttc
      661 ttgtgttcca tggtcagatg gcccgggtag acgggaatgg cgaagtcggg gcgacaactc
      721 accgtatcga tctcgtcgat cggctcgtac gcccgcgttt tgaaggccgt actcgacagc
      781 accgccagat tgccgcccgc cgagaagccc atgacgccga tcttcttcgg gtcgagatcg
      841 tacgtcgcgg cccgcgacct gacgagagag atagctctct gcgcatcctg cagagctttc
      901 ggcacccggg gcgtcacgtg cttcttcgcc ttgtcgtccc agtaacagcc cgagtgcggc
      961 acgcgatact tcagcagcac gcaggtgatt cccgcctggt tcagccacgc ggcgacagct
     1021 gcaccgtc
//
LOCUS       DAORHD010001753         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223124_length_1025_cov_3.242268, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001753 DAORHD010000000
VERSION     DAORHD010001753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223124_length_1025_cov_3.242268"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKO06_25005"
     CDS             complement(<1..246)
                     /locus_tag="PKO06_25005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72992.1"
                     /translation="MASQAEKNTSPALPHPETSAAVNRRTQIVGWFLLFLVFILIPGI
                     IFDRVVSDIANQYEEDLRQTHAQKASELYSAVENEFNV"
     gene            423..>1025
                     /gene="neuC"
                     /locus_tag="PKO06_25010"
     CDS             423..>1025
                     /gene="neuC"
                     /locus_tag="PKO06_25010"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HNV72993.1"
                     /translation="MNSRKLCIVTGTRADYGHLYWLMKDILADTDLTLQVVVTGMHLA
                     PEFGKTENNLLEDGFPIHERVDLKMTGDTPVDICRSMGLAVIGCGEAFQRLQPDLVLI
                     YGDRFEMLAAAQAAIVCRLPIAHIAGGETSEGAMDEMFRHAITKMSTYHFVTAEPHRE
                     RVIQLGEDPARVWNVGEPGLDHLTRSTFLSRRELEQSLSLA"
BASE COUNT          228 a          278 c          280 g          239 t
ORIGIN      
        1 gacgttgaac tcgttctcaa cggcggaata gagttcagaa gctttttggg catgtgtttg
       61 tcgcaggtct tcctcatact gattcgcgat atcgctgaca acgcgatcga agatgatgcc
      121 gggaatcaga atgaagacca ggaatagcag aaaccaaccg acgatctgcg tgcgtcggtt
      181 cacagcggcg ctggtttccg ggtgcggaag agcgggtgat gtgttctttt cagcctgaga
      241 agccatgaaa ctctcgatgc ggtaccattc tgtctcagaa tattttgatt ggcattttca
      301 tcacgatttt aaaacacgaa gcctcgaaaa gatagtcatt cccctcggga agaagtttcg
      361 tgtagactat cgctgacatg gccctttgcc catcactcag atcgcacgca ccggaggccc
      421 cgatgaattc gagaaaactg tgtatcgtca ccggtacgcg tgccgattac ggccacctgt
      481 actggttgat gaaagacatt ctggccgaca ccgatctcac gctgcaggtc gtggtcaccg
      541 gcatgcatct ggcacccgag tttgggaaga ccgagaacaa tctcctcgaa gatgggtttc
      601 ctattcacga gcgagtcgac ctcaagatga ccggtgatac cccagtcgac atctgccgtt
      661 caatgggact ggccgtcatc ggctgtggtg aagcgtttca gcgattacag ccggatctgg
      721 tgctcatcta cggggatcga ttcgagatgc ttgccgccgc gcaggcagcc atcgtctgtc
      781 gtctgcccat cgcccacatc gccggtggag aaacctcgga aggggctatg gatgagatgt
      841 tccggcatgc catcaccaag atgtccacct atcatttcgt caccgccgaa ccgcaccgcg
      901 aacgggttat ccaactcggc gaagatccgg cgcgggtgtg gaacgtggga gaacctggtc
      961 tggatcatct gacccgctcc acctttcttt ctcgtcgtga gcttgaacag agtctttcac
     1021 tcgcg
//
LOCUS       DAORHD010001754         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223191_length_1025_cov_2.601031, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001754 DAORHD010000000
VERSION     DAORHD010001754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223191_length_1025_cov_2.601031"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PKO06_25015"
     CDS             <1..240
                     /locus_tag="PKO06_25015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72994.1"
                     /translation="QEKKVPDRQHADALTAYRFSGEAFQVELFDSRAGQNLTQVWQEG
                     SPWAVTSESHSRKARLLPPSAPVSLPPTPNNGGER"
     gene            240..>1025
                     /locus_tag="PKO06_25020"
     CDS             240..>1025
                     /locus_tag="PKO06_25020"
                     /inference="COORDINATES: protein motif:HMM:NF027993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV72995.1"
                     /translation="MKRTILPLILACLALYSTGPLFAEDGSARGEIKPAPWSCDWWSR
                     SKGFLVKGWPGHSPSPFERYDQYVISRTGKNPGVHAWELDTRNQHYNPKAQQWEGHCN
                     GWAAASMLTPEPRERRVRNGITFETADQKGLLCEQFMNTYCMFFGKRYWSKKDDMDDI
                     YPDQFHQLLLEYIGSGKSAVIVDASCDEQVWNYPIYKFESTWSSGWLNSEKLKVKTTC
                     YFSDDNVKPDFLGTKWFSVTYTYNLFTDDNGNIIVGANGRAKRE"
BASE COUNT          230 a          295 c          289 g          211 t
ORIGIN      
        1 caggaaaaga aagtgcccga ccgccagcat gctgacgctc tcacagccta ccgattctca
       61 ggtgaggctt tccaggtcga attgtttgat tcccgtgccg gccagaatct gactcaggtg
      121 tggcaggaag gatcgccgtg ggccgtgacg agcgaatcgc actctcgcaa ggcgcgtttg
      181 ttgcccccct ctgcccctgt ctcgctgccg ccgacgccga acaacggagg tgaacgctga
      241 tgaaacgcac cattctccca ctgattttgg cctgtctcgc tctctacagc accggccccc
      301 tgtttgcgga agacggcagc gcgcgcggtg agatcaagcc ggctccctgg tcatgtgact
      361 ggtggtcacg cagcaaaggc tttctcgtca agggctggcc cggacattcc ccctcgccct
      421 tcgagcgcta cgatcagtat gtgatcagtc gcaccggcaa gaaccccggg gtgcacgcct
      481 gggaacttga tacccgaaac cagcactaca atccgaaagc tcagcagtgg gaaggccact
      541 gcaatggctg ggccgcggcg tcgatgctga ctcccgaacc gcgtgaacgg cgggtgcgca
      601 acggcattac cttcgaaacc gctgaccaga aaggattgct gtgcgagcag ttcatgaaca
      661 cctactgcat gttcttcggc aagcgctact ggtcgaagaa ggacgacatg gatgacatct
      721 atcctgatca gttccatcag ttgttgctgg aatacatcgg ttccggcaag agcgcggtga
      781 ttgtcgatgc ctcgtgcgac gaacaggtct ggaactaccc gatctacaag ttcgaatcaa
      841 cctggtcgag cggttggctt aactcggaaa aactgaaagt caagacgacc tgctacttct
      901 ctgacgacaa cgtgaaaccg gattttctgg gtacgaaatg gttttcggtg acgtatacgt
      961 acaatctgtt caccgatgac aacgggaaca ttattgttgg ggctaacggt cgggccaaac
     1021 gcgaa
//
LOCUS       DAORHD010001755         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223203_length_1025_cov_2.534021, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001755 DAORHD010000000
VERSION     DAORHD010001755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223203_length_1025_cov_2.534021"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKO06_25025"
     CDS             complement(<1..328)
                     /locus_tag="PKO06_25025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV72996.1"
                     /translation="MPIYEYFCGACRHRFTVFCKITDLEEHPACDKCRSTSTRRLVSR
                     FKTVRSEDQIMESMADPSVLSGIDENDPRSIARWAKKMAKEMGEDMGDEIEAMAEEEL
                     SKAGKTG"
     gene            419..>1025
                     /locus_tag="PKO06_25030"
     CDS             419..>1025
                     /locus_tag="PKO06_25030"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV72997.1"
                     /translation="MVEKSPYELIKSFDESLWRQGLEALVVERSPESCQAIVGVLSDK
                     IWKKRETAAKFLVEWGSDITPILCSHTATRNFDELYWLLHILGHFDHPQAAQEVRKFL
                     SHTDPEVRGYAVRALAQQKKVEQSRWLLPLLNDQNWAVRKLAFECLMGFGQPLLAELR
                     PLLFAEKDEPQHSIVALYVKIGGDAVLQDLNQLYKDSTFAVR"
BASE COUNT          233 a          261 c          271 g          260 t
ORIGIN      
        1 cgcctgtttt tcccgctttt gacaactctt cttcggccat ggcttctatt tcgtctccca
       61 tatcttcacc catttctttt gccatttttt tcgcccaacg ggcgattgac cgcggatcgt
      121 tttcatcgat tccggaaaga acggatggat cggccatgga ttccatgatc tggtcttctg
      181 agcgcacagt cttgaagcgg gaaaccagac gccgggtgct ggtcgagcgg cacttatcac
      241 aggcggggtg ctcttcgaga tctgtgatct tgcagaagac ggtgaaacga tgacggcatg
      301 cgccacaaaa atactcgtaa ataggcatca tcgaactcct ctggttttct ttctccaaga
      361 tacggattat aatagattaa agtgtcgcag gcaatatgac cgataatgta cagagagtat
      421 ggtcgaaaaa tcgccgtatg agctgatcaa gagctttgat gaatcgctct ggcgccaggg
      481 cctggaggcc ttggtggtcg agcgttcgcc agaatcctgt caggctatcg tcggggttct
      541 ttccgacaaa atctggaaaa agcgcgaaac tgccgcaaaa ttcctggtgg aatggggcag
      601 tgatattacc ccgatactct gctcccacac cgcgaccaga aacttcgacg aattgtactg
      661 gctgttgcac attcttggcc atttcgatca tccgcaggct gcgcaagagg tcagaaagtt
      721 cttgtctcac accgacccag aggttcgtgg ttacgctgtt cgggccctgg cacagcagaa
      781 gaaggtcgag caaagccgct ggctgttgcc tttgctgaac gatcagaact gggcggttcg
      841 caagctggcg tttgaatgcc tcatgggatt cggacagccg ttgctggcag agctgcgtcc
      901 attgctcttt gccgagaaag acgagcctca acattcaatt gtcgccctct acgtgaaaat
      961 cggtggcgac gcggttctgc aagatctcaa tcagttgtat aaagacagca cgtttgccgt
     1021 gcgat
//
LOCUS       DAORHD010001756         1025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223254_length_1025_cov_2.225773, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001756 DAORHD010000000
VERSION     DAORHD010001756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223254_length_1025_cov_2.225773"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PKO06_25035"
     CDS             complement(<1..758)
                     /locus_tag="PKO06_25035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV72998.1"
                     /translation="MNSQSIGVGFVSGASLTLTRRCRHRCGYCSFRLQETGVPLLSLP
                     QIETDLNAIVRMGITEVTFEAGEAPQEFPEVVLALRENGCESLGEYLTKVSAACLARN
                     LLPVFSLGEISPLEARQLAQSAAMQKICVVAADLRAPGEAHEEARGRHPAAARHQIEI
                     AHEAGLPYRLSFLIGIGEHHEERERAIVDFGRFCAADPLLQDVELHPFQPQSVTAMRG
                     RPPLPFAAVAQAMSQLAKAFPVHCRSIAPHLFYH"
BASE COUNT          227 a          299 c          317 g          182 t
ORIGIN      
        1 taatgataga acaggtgcgg ggcgatcgag cggcaatgaa ccggaaaggc tttcgcaagt
       61 tgtgacatgg cctgggcaac agcggcgaaa ggcaggggcg gccgcccacg catcgcggtg
      121 acactctgcg gctgaaacgg atgcagttcg acatcctgca gcagagggtc ggccgcgcag
      181 aatcgaccaa agtcgacaat ggctcgttcc cgctcttcgt ggtgttcccc gatgccgatc
      241 agaaacgaca accgatacgg caacccggct tcgtgtgcga tctcgatctg atgacgagcc
      301 gcggccggat gccgcccgcg cgcctcttcg tgggcttcac caggtgccct gagatcggcg
      361 gcgaccacgc agattttctg catggccgca gactgtgcga gctgcctcgc ttcaagcgga
      421 ctgatctcgc ccagcgaaaa gaccggaagc agattgcggg ccagacaggc ggcagagacc
      481 ttggtgagat actcaccgag cgattcacag ccattctcgc gcagggcgag gacgacctcc
      541 gggaactcct gtggggcttc tccggcttca aaggtgactt cggtgattcc catgcggacg
      601 atggcgttca aatcggtttc gatctgcggc agcgatagca gcggaacacc ggtctcctgc
      661 aggcggaacg aacagtagcc acagcggtgc cggcaccggc gggtgagcgt caggctggcg
      721 ccggaaacga atccgacacc aatcgattgg gaattcatgt ccggatttta acacaccgac
      781 tgaccggtgg aaacttttcg cgaggcttgc atcatccgtg tcgaattcgg ggggacgttg
      841 ttgccatcac acgagctcgt cgacggtctg acaatctcgc gttacgagct ggtactgctt
      901 ccgccgaacg accacaacag atacgaagca agggtgcgca accctcccgt aagcccgaga
      961 aaagcgttca tcggaatcag acatttcaag accagagtcg cacggaccgg aattgtccag
     1021 tagtg
//
LOCUS       DAORHD010001757         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223479_length_1024_cov_3.694530, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001757 DAORHD010000000
VERSION     DAORHD010001757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223479_length_1024_cov_3.694530"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1024
                     /locus_tag="PKO06_25040"
     CDS             12..>1024
                     /locus_tag="PKO06_25040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV72999.1"
                     /translation="MVKHLSEVMGGKLYAEILPQVESCLGGKRMTFETERDHPILGRR
                     NVALSYFPLLGTGTQPIGMVSYLKDITDRKRREAEARRRQDQLAQVEKLVSLGTMVSG
                     VAHEINNPNSFVMTNSTILAKVWEGTLPILDAWFRENGDFLLGGVPYGKQRERVAELI
                     NGIRSGGERIRNIVDELRRFSRSEAGQEQIRLNLNEPIMAAVTLTSNVIRKATHRFSM
                     SLATSLPLIMGSPQRLEQLLINLIQNGCQALTSPEQSVRVESGVDQSSRRVFVRVQDD
                     GAGIPAHLLQRICDPFFTTRRDSGGTGLGLSISARIVEEHRGEMRFDSVPGRGTTVTI
                     YLPP"
BASE COUNT          220 a          288 c          308 g          208 t
ORIGIN      
        1 gagaagaggt catggtgaaa catctttccg aggtcatggg ggggaaactc tatgccgaga
       61 ttctgcctca ggtcgagagt tgtctgggcg ggaagcgcat gaccttcgaa accgaacgag
      121 accatccgat tcttgggcgg agaaatgtcg cactgagtta ttttcctctg cttggaactg
      181 ggactcagcc gatcggcatg gtttcctatc tgaaggacat cacggatcgc aaacgccgtg
      241 aggcggaggc ccgccgtcgc caggatcaac tggctcaagt ggaaaagctg gtgtcactgg
      301 gaacaatggt ctccggcgtt gcccatgaaa tcaacaatcc caactctttc gtcatgacga
      361 actcgaccat tctggccaag gtgtgggaag gaactctccc gattctcgat gcctggttcc
      421 gcgagaacgg cgactttctt ctgggaggag tcccatacgg caaacaacga gaacgagtcg
      481 ccgagctgat taacggcata cgcagtggtg gcgagcgcat ccgcaacatc gtggacgaac
      541 tccgtcggtt ttcgcgcagc gaggcagggc aggaacagat tcgtctgaat ctgaacgaac
      601 ccatcatggc ggccgtgacg ctgaccagta acgtcattcg caaggccaca caccggttca
      661 gcatgagtct ggcgacatcg ctgccactga tcatgggctc gccccagcgg cttgaacagt
      721 tgttgatcaa cctgattcag aatggatgtc aggcgttgac gtcgcccgaa cagtccgtgc
      781 gggtcgagtc cggtgtcgac cagtcgagtc gccgggtgtt tgtacgcgta caggacgacg
      841 gggctgggat tcccgcgcac cttctgcaga gaatatgtga cccgttcttc acgaccagac
      901 gtgattccgg cggcacggga ctgggacttt cgatctcggc ccgcatcgtc gaggaacatc
      961 gcggcgagat gcgcttcgac tcggtccccg gtcgtgggac caccgtgacc atctacctgc
     1021 cccc
//
LOCUS       DAORHD010001758         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_223906_length_1023_cov_3.113636, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001758 DAORHD010000000
VERSION     DAORHD010001758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_223906_length_1023_cov_3.113636"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PKO06_25045"
     CDS             complement(<1..981)
                     /locus_tag="PKO06_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73000.1"
                     /translation="MIGFLRLIQLDESRLALFPVSHDSETMMGTNWLIFILVWGAWII
                     VMGLLLLGNIDNQSTMSLANKGALLLIIAGILPTLGLYWSYRSLSRWQSVLATSQGYN
                     ILNQRLINFESQQEQKISRVVTEIQRFLEHPMWVKGDYTPEKIASLTHEINKDVVQSL
                     EMPIRPNIVHRETGTVFLAAKYFGYANTLVEVNTIELIENLLVALQQTFARASGKQAI
                     SMGNVKATVLLDALQSLLGAANLYRLAMSTDRLDGHRLMGETLWSFLHLMRDEQRKVI
                     MSFFITLERKGLQRRQIYSWNEEMRQSFNERPQEQVPLCGFGLNMQSDYII"
BASE COUNT          237 a          274 c          275 g          237 t
ORIGIN      
        1 aatgatgtaa tcgctctgca tgttgagacc aaagccacag agtgggacct gctcctgcgg
       61 tcgctcgtta aaactctgcc gcatctcttc gttccaggaa tagatctgac ggcgttgcag
      121 cccttttcgc tcaagcgtga tgaagaacga cataatgact ttgcgttgtt cgtcgcgcat
      181 cagatgtaaa aaggaccaga gagtttctcc catcagtcgg tggccatcga ggcggtccgt
      241 gctcatggcc agccggtaca aattcgctgc gccgagcagt gactgcaagg catcgagcag
      301 aacggttgcc ttgacgttgc ccatggagat cgcctgttta ccgctggctc gggcgaaggt
      361 ttgctggagc gcaaccaaca gattctcgat cagctcgatg gtattgacct caaccagcgt
      421 gtttgcatag ccgaaatatt tggccgcgag gaataccgta cctgtttcgc gatgaacgat
      481 gttcggtctg atcggcatct ccagtgactg aacgacatcc ttgttgatct cgtgcgtgag
      541 actggcgatc ttctctggag tatagtcgcc cttcacccac atcggatgct cgagaaagcg
      601 ctgaatctcg gtaacgaccc gggaaatctt ttgttcctgc tggctctcga aattgatgag
      661 ccgctggttg agtatgttgt atccctgaga tgtggcaagc actgactgcc agcggctcaa
      721 agaacgatac gaccagtaca atccaagcgt tggcagaatt ccggcaatga tcagcagcaa
      781 agcgcccttg ttcgcgagcg acatggtgga ctggttgtcg atgttgccca gcaacaggag
      841 ccccatcaca atgatccacg ccccccagac caggatgaag atcaaccagt tggtccccat
      901 catggtttct gaatcgtgtg aaaccggaaa cagagcgagg cgactctcgt cgagctggat
      961 caaacggaga aagccgatca tctgactttc gctgtccacg atccctgaac ttgtcgatgc
     1021 cag
//
LOCUS       DAORHD010001759         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_224634_length_1021_cov_3.135611, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001759 DAORHD010000000
VERSION     DAORHD010001759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_224634_length_1021_cov_3.135611"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKO06_25050"
     CDS             <1..196
                     /locus_tag="PKO06_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNV73001.1"
                     /translation="EKQHEKRYKKLLQNLESGKVFKKDQKTTWQCRNCGFVHEGPEAP
                     QACSACLHPQAYFEMIAENW"
     gene            complement(266..>1021)
                     /locus_tag="PKO06_25055"
     CDS             complement(266..>1021)
                     /locus_tag="PKO06_25055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73002.1"
                     /translation="KAPELSMRPFALSLSVTAESRPSDGKVLFFCTMLFKLTHGTQAD
                     KLAIDMKHDLLSTVFEQIKGEPGVKKKVQVEGVNTWLTNLRISTEKSGNRMQITGYAF
                     GFQEMSKVYRGLLDSGKAPQTMLSNCNANTYYGFPVLRFDLVSGDSTKAEAQVALSQA
                     VIAMNDAVEVANTSIQDLRISPAIKTDKGFDYPMEVSFKQMEIDQWEQVKTKLSGYAS
                     HGAKVQKIGELKALADEGSKMVQLIVTCDLDAK"
BASE COUNT          233 a          306 c          252 g          230 t
ORIGIN      
        1 cgagaaacag cacgagaagc gctacaagaa gctcctgcag aacctcgaat ccggcaaggt
       61 gttcaagaaa gaccagaaaa ccacctggca gtgccgcaac tgcggtttcg tgcatgaagg
      121 ccccgaagct ccgcaagctt gctctgcctg tctgcacccg caggcgtact tcgaaatgat
      181 tgctgaaaac tggtaagcaa ccgcaataac tgttaaaaaa gcgaggggcg caggcctctc
      241 gcttttttat tccggtcaga tcgtcttact ttgcgtcgag atcacaggtg acgatcaact
      301 gaaccatctt actgccttca tccgcaagag ccttcagctc gccgattttc tgcaccttgg
      361 ctccgtgtga cgcgtagccg ctcaacttcg ttttcacctg ctcccactgg tcgatctcca
      421 tctgtttgaa cgagacttcc atcgggtaat cgaaaccctt gtccgtcttg atggcgggcg
      481 aaatgcgcag atcctgaatc gacgtgttgg caacctctac cgcgtcgttc atggcaatga
      541 cggcctgaga cagagcgacc tgagcctcag ccttggtgct atctccggac accaggtcaa
      601 aacgaagcac cggaaacccg tagtacgtat tggcgttgca gttcgatagc atggtttgcg
      661 gggctttgcc gctatccaac agcccgcggt acaccttgct catttcctga aagccgaacg
      721 cgtaccccgt gatctgcatg cggtttccag acttctcggt cgagatgcgc aggttggtca
      781 accacgtatt cacgccctca acctggactt tctttttcac ccccggctcg cccttgatct
      841 gttcgaacac ggtcgacaac agatcatgtt tcatatcgat ggccagttta tccgcctgcg
      901 ttccatgcgt gagcttgaac aacatggtac aaaagaagag aaccttgcca tcactcgggc
      961 gagactcagc cgtcaccgac aacgacagcg caaacgggcg catcgacagc tccggggcct
     1021 t
//
LOCUS       DAORHD010001760         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_225016_length_1020_cov_2.862176, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001760 DAORHD010000000
VERSION     DAORHD010001760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225016_length_1020_cov_2.862176"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKO06_25060"
     CDS             <1..310
                     /locus_tag="PKO06_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73003.1"
                     /translation="LFKKMRGAIIVCAPTRFEATLDTYRMVVVPHPTNPKKVLSSFSG
                     DDPQVPQQPIGAVITTNGTTAKIIGDDGTTVEVTYNGGVSYTFVSNKLPVKVSAKYVD
                     "
     gene            393..>1020
                     /locus_tag="PKO06_25065"
     CDS             393..>1020
                     /locus_tag="PKO06_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73004.1"
                     /translation="MSNPSLTIIQGGKLLCLESGALTEHLSPFAQEKVAQEARDTSRH
                     AWKTGDEAGNQHLFSRSQLWGGKGEAQPVRPPRFAWAQRQSSILWYVLNLSRTRGLFR
                     FDVKAGQESRLFHHDGFDPHGLCLASDGGFFTTRTNADGAMHIVRLDETGSAKQVVTD
                     GDCLDEFPCLNGNDLYYQTSGLGRNDQGFVCAQAATAVYRIDLTSGRTE"
BASE COUNT          209 a          297 c          301 g          213 t
ORIGIN      
        1 tctgttcaag aaaatgagag gggctatcat cgtctgcgcc ccgacccgtt tcgaagcgac
       61 cctcgacacc tatcgcatgg tcgtggtgcc gcacccgacc aatccgaaga aggtgctttc
      121 gagcttttcc ggtgacgatc ctcaggtgcc tcagcaaccg atcggagcgg tgattaccac
      181 gaacggcacg accgcgaaga tcatcgggga cgacggcacg accgtcgaag tcacctataa
      241 cggcggcgtg tcctatacgt tcgtcagcaa caagcttccc gtcaaggttt cggcgaaata
      301 cgtcgactga tcagttcgca cgcgaaaacc gctggaatgg ccatttcggc ggttttttcc
      361 tgtcggaacg ttcgtgatag actgtgcccg tcatgagcaa cccaagtctc accatcattc
      421 aagggggcaa gcttctctgc ctcgaatccg gcgcgctgac ggaacatctc agcccctttg
      481 cccaggaaaa agtcgcgcag gaagctcgtg atacctcgcg tcacgcctgg aaaaccggcg
      541 acgaggccgg caatcagcat ctgttttcgc gcagtcagtt gtggggtggc aagggagaag
      601 cccagccggt tcgtcccccg cgctttgcct gggctcaacg acaaagctcg atactgtggt
      661 atgtgctgaa cctgtcacgg acccgcgggc tgttccgctt cgatgtcaaa gcgggccagg
      721 aatcccggct gtttcatcac gacgggtttg atccgcacgg gctctgtctg gcttctgacg
      781 gaggcttctt cacgactcgt acaaacgcgg acggcgccat gcatattgtg cgcctcgatg
      841 aaacgggttc ggcgaagcag gtcgtgacag atggtgattg ccttgatgaa ttcccctgtt
      901 tgaacggcaa tgacttgtac tatcagacct ccgggctggg gcgcaatgat caggggtttg
      961 tctgcgcgca ggcggcaacg gcggtctacc gcatcgatct gacgagtggg cgcaccgagg
//
LOCUS       DAORHD010001761         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_225940_length_1018_cov_2.116303, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001761 DAORHD010000000
VERSION     DAORHD010001761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225940_length_1018_cov_2.116303"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1018
                     /locus_tag="PKO06_25070"
     CDS             <1..>1018
                     /locus_tag="PKO06_25070"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV73005.1"
                     /translation="NLCRLLSDPDEWIVINVLEILSQLKESEAIPALVGQFEIAQDAR
                     LKAIIISSLSTFREPKLLHLFEEGLKSFDPRIQANAVEALANLKIPALELKRKLKKFL
                     THTNNRVRANAAIALSPIEPEAVKTEIEKMLNSSDVPTRRSAAYVLSKVQMADRDLMI
                     DRLLADSSYMVRKIALKAALVMDTEIGTNRILPLLKDKNPWVRKEAVECGRKISRFPV
                     DPVLELFKGENNPPVIESILDFVVDRRLNQAVALIVAKIKAQPEESLGRMIATLGRLQ
                     AREALFDVRKYLPPSDDTIMSRFYVALLLHGELNVFQELQQSLAGRPRDTDLLMLIKA
                     AGEIG"
BASE COUNT          221 a          289 c          291 g          217 t
ORIGIN      
        1 gaatctgtgt cgcctgcttt ccgaccctga cgagtggatc gtcatcaacg tgctcgagat
       61 tctgtcgcaa ctgaaagagt ccgaggcgat tccggcgctg gtcgggcagt tcgaaatcgc
      121 gcaggatgcg cgtctcaagg ctatcatcat ttcctcgctg agcacctttc gcgaaccgaa
      181 gttgctgcat ctgttcgaag aagggttgaa gtcgttcgac ccgcgcattc aggccaatgc
      241 cgtcgaagcg ctggcgaatc tgaaaattcc ggccctcgaa ctcaagcgca aactgaagaa
      301 gtttctcacg cacaccaata atcgcgtgcg ggccaatgcc gcgatcgcgc tttccccgat
      361 cgaacctgag gcggtcaaaa ccgaaatcga gaagatgctg aacagcagcg acgtgccgac
      421 ccgtcgttcg gcggcctatg tgttgtcgaa agtgcagatg gccgatcgcg atctgatgat
      481 cgaccgtctg ctggccgatt cgtcctacat ggtgcgtaag atagctctga aagcggcttt
      541 ggtcatggat actgaaatcg gcaccaatcg tattctgccc ctgttgaaag acaagaatcc
      601 gtgggtgcgc aaagaagcgg tcgagtgcgg tcgcaagatc tcgcggtttc ccgtcgaccc
      661 cgtgctcgag ctgttcaagg gtgagaacaa cccgccggtc atcgaaagca tcctcgactt
      721 cgtcgtcgat cgacgtctga atcaggccgt tgccctgatt gtcgccaaaa tcaaagcgca
      781 gcccgaagag tcgctcggca ggatgattgc cactctgggc cgtctgcagg ctcgtgaagc
      841 gctgttcgac gtgcgaaaat acctgccgcc gtcagacgat acgatcatgt ctcggttcta
      901 tgtggccctg ctgttgcatg gcgaactcaa cgtctttcag gaactgcaac agagcctggc
      961 tggtcggccg cgcgacaccg atctgctgat gctgatcaag gcagccggtg aaatcggc
//
LOCUS       DAORHD010001762         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_226904_length_1015_cov_4.519792, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001762 DAORHD010000000
VERSION     DAORHD010001762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_226904_length_1015_cov_4.519792"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..>1015
                     /locus_tag="PKO06_25075"
     CDS             297..>1015
                     /locus_tag="PKO06_25075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73006.1"
                     /translation="MTQALPPKPSVEPKPVSAAAPTRSVTSNQVAAEPATGLCRDIDV
                     LIRARYPILYVVTHEEERAEFALFQIARAQNKQCLSWSVTRGLMPTGTDMQSKKKISE
                     MTRDPLAALESVVENLEHALFIFKDFHPYLQDTTIIRKVRELAGFLRDSPRTLIFISP
                     VLRIPTELEKEITVLEFGLPMPKEIAQKLDEMVDQVKDNPKVSISLTQEDREKLVKAC
                     TGLTLREVENVLAKTIISTNQ"
BASE COUNT          253 a          260 c          296 g          206 t
ORIGIN      
        1 tgacaatggc aatgaccaga ccgaggaaga gcacgagtcg ccagcctgga gaaagagggg
       61 ttggtctgac gaagcgcttc ggaggcatat ggtgctcctg tgtaaggtcg aggcgacagt
      121 ggatcagtga tagcacgcag atcgatgaaa attcaatgcc gtacagcagg ctgcgatgtc
      181 tgaagcgaaa caggtcatgg gaatggaaat gaccttgatt ggccgcaaat gattttgact
      241 ttcacgctgc acgattgcta gaattcagca tcttgagctg aatctgcgag ggagacatga
      301 cccaggccct tccaccgaaa ccgtctgtcg aaccgaaacc agtctctgcc gctgccccca
      361 cccgcagcgt gacgtcgaat caggtcgccg ccgagccggc tacgggactg tgccgcgaca
      421 tcgatgtgct gattcgggcc cgatacccga ttctgtatgt cgtcactcat gaagaggaac
      481 gagcggagtt tgccctgttt cagattgccc gggcacagaa caagcagtgc ctgagctggt
      541 cggtgacccg tggtctgatg ccgaccggaa ccgatatgca gtcgaagaaa aagatttcgg
      601 agatgacccg agatccgctg gcggcgctgg aaagcgtggt ggaaaatctc gaacatgcgt
      661 tgttcatctt caaggacttt cacccgtatc tgcaggatac gacgatcatt cgcaaggtcc
      721 gcgaattggc gggttttctc cgtgacagtc cgcgcacgct gattttcatt tcacccgttc
      781 tgcgtattcc cacggaactc gaaaaagaga tcacggtact cgagttcggg cttcccatgc
      841 cgaaagagat tgctcagaaa ctggacgaaa tggtcgatca ggtgaaggac aacccgaagg
      901 tgagcatctc actgactcag gaagaccgcg aaaagctggt gaaggcttgc accgggttga
      961 ccctgcgtga agtcgagaat gtgctggcga agacgatcat ctccaccaat caaag
//
LOCUS       DAORHD010001763         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_228149_length_1012_cov_3.940439, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001763 DAORHD010000000
VERSION     DAORHD010001763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228149_length_1012_cov_3.940439"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1012
                     /locus_tag="PKO06_25080"
     CDS             29..>1012
                     /locus_tag="PKO06_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73007.1"
                     /translation="MRFRIGVLDSATGAGAPGFPVQARVIDPSGITVIKRDVVIGYQG
                     TGDFRALFHRRSRAGTYRFEFVAGDRTLVWEIPLLASPDEKVIERRVNQFLRGFLPIP
                     LGSANAAERFVTRFESPHPDPEREVTLEVQKEPRFWVFADRAPHDVGDVWECWFRNRF
                     LMTSGLAHPLPLKASIERTGDRTGILAFSRWNVRGPGREQRFLFPAMAETTPARECFR
                     DLTLMVENPRDEVYRALLNPGDPSSIEGRIYGGDSKTVVWVRRSGRILLAFLGCIIGL
                     YLLIPAYMPGVAATQLAGLLLLAFLVAGRQALPDWLFVCLCGVLFVAGFWPA"
BASE COUNT          165 a          301 c          304 g          242 t
ORIGIN      
        1 ccctggaccg ctacaactcg gtgacgacgt gcgttttcgc attggggtgc tcgacagcgc
       61 gaccggagcg ggtgcgccag gttttccggt gcaggcccgc gtcatcgacc cttcgggaat
      121 cacggtgatc aagcgcgatg tcgtgatcgg gtatcagggc accggtgatt ttcgcgccct
      181 gtttcatcgc cgcagcagag cgggaaccta tcgtttcgaa ttcgttgccg gggatcggac
      241 gctggtttgg gaaattccgc tgctggcctc tcctgacgag aaggtaatcg aacgtcgcgt
      301 gaatcagttc ctgcgcggtt ttttgccgat tcccctgggg tcagccaacg cggcggaacg
      361 cttcgtgacc cgcttcgaat ccccccatcc ggacccggaa agagaagtca ccctggaggt
      421 tcagaaagaa cctcgtttct gggtgtttgc cgaccgtgcg cctcacgacg tgggtgatgt
      481 ctgggaatgc tggtttcgca accgtttcct gatgacctct ggcctcgctc atcccctgcc
      541 cctgaaggcc tcgatcgaac gcaccggtga tcgcaccgga attctggcat tctctcgctg
      601 gaatgttcga ggtccgggca gagaacagcg gttcctcttc ccagccatgg ctgagaccac
      661 acccgctcgc gagtgtttcc gcgatctcac gctgatggtc gaaaatcctc gtgatgaagt
      721 ctatcgcgcg ttgctgaacc cgggagatcc gtcatcgatc gaaggacgta tatacggtgg
      781 cgacagcaaa accgttgtct gggtcagacg ttccggccgc attctcctgg catttcttgg
      841 ctgcatcatc ggcttgtatc tgctcattcc ggcatacatg ccgggtgtcg cggcgacaca
      901 acttgccgga ctgttgctgc tcgcgtttct ggtggccgga cgacaggctc tccctgactg
      961 gctttttgtc tgtctgtgcg gagtgctctt cgtagccggt ttctggccgg cg
//
LOCUS       DAORHD010001764         1010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_228968_length_1010_cov_3.493194, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001764 DAORHD010000000
VERSION     DAORHD010001764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_228968_length_1010_cov_3.493194"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1010
                     /gene="flgL"
                     /locus_tag="PKO06_25085"
     CDS             <1..>1010
                     /gene="flgL"
                     /locus_tag="PKO06_25085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNV73008.1"
                     /translation="QLQISSGKNYLTPSQNVVGNALSMHYKTEIVEHTQYMSNASRSV
                     DWLNQTDTALTTIESVLQRARELAVQGANDTLVQEDRDAIAKEINQLMEHLVGVANTD
                     IGGEFLFAGAKVNTKPFDSITGQKPNTMKDIVTFSAGKTRTDLNLNSLLDVGYLGNNL
                     KLVSEIEKGIVLERSLTGLEVFYGDDQVSPQPSFVESMPPISKSLSLQALNNGRGVQA
                     GIILATDSNGVETAIDLKSARTIDDVIQIIKLTRGFEAGLEEVPSQAAEALGLLRNVG
                     PSSLLIGQSDAAMLSTGTALSSLNNGLGVPGGYLSINTSDGKNHRIDLSTAGTVQDVL
                     DL"
BASE COUNT          238 a          281 c          297 g          194 t
ORIGIN      
        1 cgcagctgca gatcagttcg ggaaaaaact atctgacgcc ttctcagaac gttgtcggca
       61 atgcgctgtc gatgcactac aaaacggaga tcgtcgagca tacgcagtac atgtcgaatg
      121 cgtccaggtc ggtcgattgg ctcaaccaga cggatacggc gttgaccacg attgaatcgg
      181 tgctgcagcg agcccgcgag ctggcagtgc agggtgccaa cgacacgctg gtgcaggaag
      241 accgtgatgc gattgccaaa gagatcaatc agctgatgga gcacctggtc ggagttgcca
      301 acacggacat tggcggggag ttcctgttcg ccggggccaa ggtgaacacg aagccgttcg
      361 actcgatcac cggccagaag ccgaacacga tgaaagacat tgtgaccttc tctgccggca
      421 agacacgcac ggacctgaac ctgaacagcc tgctcgacgt cggctatctc ggcaacaatc
      481 tgaaactggt ttccgagatc gagaaaggaa ttgtcctcga gcgcagtctg accggtctcg
      541 aagtgttcta tggagacgat caggtctcgc cgcagccttc ctttgtcgaa tcgatgccgc
      601 ccatttcaaa atcgctcagc ctgcaggcac tgaacaatgg caggggcgta caggccggca
      661 tcattcttgc cactgattcc aacggagtcg agacggcgat cgatctgaaa tcggcacgca
      721 cgatcgacga cgtcattcag atcatcaagc tgacccgtgg gtttgaagca gggctcgaag
      781 aggtgccaag tcaggcagca gaagccctgg ggcttctgcg caacgtcggg cccagcagtc
      841 tgctgattgg tcagtccgat gcggcgatgc tttcgaccgg cacggcattg agcagcctta
      901 acaacggtct gggtgttccc ggcgggtatc tcagcatcaa caccagcgat ggcaagaacc
      961 accgcatcga tctttcaacg gctggaaccg tgcaggatgt gctcgatctg
//
LOCUS       DAORHD010001765         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229788_length_1008_cov_3.809024, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001765 DAORHD010000000
VERSION     DAORHD010001765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229788_length_1008_cov_3.809024"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /gene="ccsA"
                     /locus_tag="PKO06_25090"
     CDS             <1..572
                     /gene="ccsA"
                     /locus_tag="PKO06_25090"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV73009.1"
                     /translation="AASLAIEWRSREVYFSLFVLPLSIPMVLLAMLRTNMLEGPEFGG
                     PWFLLHIFGTIAGECFFFLAALASATYLYLVRNLKQKNRGRALFFFPPLSRLDSLITA
                     FTGVGLGLFFLGLFTGGIWSMNRYGALALHHPKVQGALAIFLLVGGLLSARLSTGWAG
                     PRWAWACLLSFCLSIILIFGIDGSLHWRP"
     gene            569..>1008
                     /locus_tag="PKO06_25095"
     CDS             569..>1008
                     /locus_tag="PKO06_25095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNV73010.1"
                     /translation="MNTMEFFPLFLRLHDQPVLVIGGGRVAERKVRHLVAAGARVTVI
                     APTVSAAITAIAHSCHVRPATPDDIQPDFRLVILATDSSALHDQLSKRCSQMNILYNR
                     GDDALQSEFVIGSPIDSSPFVGAVIASGSPGVGRLARARMEAVL"
BASE COUNT          163 a          300 c          285 g          260 t
ORIGIN      
        1 ttgccgccag ccttgccatc gagtggcgaa gccgcgaagt gtacttcagc ctgtttgttc
       61 ttcccctgtc aattccgatg gtgcttctgg caatgctgcg caccaacatg ctcgaaggtc
      121 ccgagtttgg cggcccctgg tttctgctgc acattttcgg cacgatcgcc ggtgagtgct
      181 tcttctttct cgccgccttg gcgtcggcaa cctatctcta tctcgtacgc aatctgaaac
      241 agaagaaccg gggaagagca ctgttcttct tcccgccgct ttcgcgactt gattcgctga
      301 tcaccgcctt caccggggtt gggttaggcc tcttctttct cggcctgttc acgggcggaa
      361 tctggtcgat gaaccggtac ggagctctcg ccttgcatca cccgaaagtg cagggagcgc
      421 tggcgatttt tctgctggtt ggcgggttgc tgagtgcgcg tctcagtacc ggctgggctg
      481 gaccgagatg ggcctgggcg tgtctattga gcttctgcct ctcaatcatc ctgattttcg
      541 gcatcgatgg aagtctgcat tggagaccat gaacacgatg gaattttttc cgttgtttct
      601 gcgactgcat gaccagcccg tgctggtgat cggcggcggt cgtgtcgcag aacgaaaggt
      661 tcgtcatctc gtcgccgcag gagctcgggt aacggtcatt gcccccactg tttcggccgc
      721 aatcacagcc atcgcccact cctgccacgt tcgtcccgcc acgcctgatg acattcagcc
      781 cgatttcaga ctcgtcattc tcgccaccga tagctctgcg cttcacgatc agctatcgaa
      841 gagatgttcg cagatgaata tcttgtacaa tcgcggcgac gacgcccttc agtccgagtt
      901 cgtgatcggt tctccgattg acagttctcc gtttgtgggc gcggtgatcg cttctggctc
      961 accgggggtc gggcgactgg cgcgagcacg catggaagcg gtcctcga
//
LOCUS       DAORHD010001766         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229791_length_1008_cov_3.728227, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001766 DAORHD010000000
VERSION     DAORHD010001766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229791_length_1008_cov_3.728227"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PKO06_25100"
     CDS             complement(<1..213)
                     /locus_tag="PKO06_25100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73011.1"
                     /translation="MHLLFRSDRRRWPLLLNCRLVIFGLVFCCLLITGAWGQELKRLP
                     VVPDFDFDRLIASYPGFAEIASIAREH"
     gene            complement(216..>1008)
                     /locus_tag="PKO06_25105"
     CDS             complement(216..>1008)
                     /locus_tag="PKO06_25105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73012.1"
                     /translation="IGVDAYTDQSILLLKTHIHLLRRLGLRAEPSYTFADIEAVLSGI
                     EQEGLTSRCFLLLNNPFLDDGDRLRTFYHLYRLALRLPGFRIDYESSSRVNELKPFPG
                     APLTLVADRLPGLVHGDRFLVPTWLPQLEHILGFELFGERRFDESAQARLVAGALEVR
                     WQICEYLRQMVAVQKEPAQSAAVAAACDFLDLEEWLKPLLRDAMRGMPGLQTREQNLA
                     EERARLKLAVSAYPSPSPTRSTERLEEFFRLLKEVVPHQEQAQGG"
BASE COUNT          251 a          313 c          267 g          177 t
ORIGIN      
        1 gtgttcacga gctatggaag ctatttccgc aaaacctgga taggacgcaa tcaaccgatc
       61 aaagtcaaaa tcgggcacga cgggcaagcg cttgagttcc tggccccatg caccggtgat
      121 cagcaaacag cagaagacca acccgaagat cacaagtcgg cagttcagca gaagtggcca
      181 ccgtcgacgg tcagaacgga agagaagatg catactcaac ccccttgtgc ttgctcctga
      241 tgcggaacaa cttctttcaa cagacggaag aactcctcaa gacgctcggt cgatcgcgtt
      301 ggtgatggcg agggatacgc actcaccgcc agtttcaatc gcgcccgctc ttcagccaga
      361 ttctgttcgc gagtctgcag gccgggcatt ccgcgcatgg catcccgcaa cagaggtttc
      421 agccactctt ccaggtcaag gaagtcacag gccgccgcca cggcagcgct ctgcgccggc
      481 tctttctgaa ccgcgaccat ctgacgcaga tactcgcaga tctgccatcg cacctccagg
      541 gctcccgcca ccagacgcgc ctgagcggac tcatcgaagc gtcgctcccc aaagagctcg
      601 aaaccaagaa tatgctccag ttgcggcaac caggtcggaa ccaggaaacg atccccatgc
      661 accagcccgg gcagacgatc ggcaacgagt gtcagaggag ctcccgggaa cggcttcaac
      721 tcgttgaccc gcgacgacga ttcgtaatcg atacgaaatc ccggcagacg caatgccaat
      781 cggtacaagt gataaaacgt tctcaacctg tctccgtcat cgagaaaggg gttgttgagc
      841 aacagaaagc accgtgacgt cagcccttcc tgttcgatgc ccgacaacac cgcctcgatg
      901 tccgcaaagg tgtaggatgg ttctgcccga agccccagcc ggcgcagcag atgtatatgg
      961 gtcttcagca gcagaatcga ctggtctgtg tacgcatcga cgccaatg
//
LOCUS       DAORHD010001767         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229832_length_1008_cov_3.084995, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001767 DAORHD010000000
VERSION     DAORHD010001767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229832_length_1008_cov_3.084995"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1008)
                     /locus_tag="PKO06_25110"
     CDS             complement(<1..>1008)
                     /locus_tag="PKO06_25110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73013.1"
                     /translation="YFYLSAPDRKGMLILFCRTPEQPVLPALSEVAAEVQRRYRGIRL
                     NIIPSEGEPPSEWLASAATSVPASEAWNLLQRNASGSVWLDGTLISRQILSPSWWAIG
                     TATLARQSGRGASRREWLGWALVLAGICCLAWFLEPWFAGSIRPKLLGAFLYASMIPL
                     LIMGFAARQFLEERRMILEQERHHEVEQGLFRLDRLYSKEVNRLSWAFLEQYAWSSTR
                     LVGVENRLAQVIPEFAKQWGFEYCAVLGSRGESLFTFRAPDGRTMSPKFLQYLGKLAG
                     ESMKRSILGEQSDRSGVRDLGTLEFLGLTPQVKQLDWGASLNIVGFFPLGTDTGRVTH
                     VA"
BASE COUNT          230 a          332 c          276 g          170 t
ORIGIN      
        1 cgctacgtgg gtgacccgac cggtatccgt cccgagcgga aagaagccca cgatgttcaa
       61 cgacgcgccc caatcaagct gcttgacctg cggtgtcagt cccagaaact ccagagtacc
      121 aagatctcgc accccactgc gatcagattg ctctcccaga atcgagcgct tcatcgactc
      181 tccggccagt tttcccaggt actggagaaa cttcggactc atcgttctgc catcgggagc
      241 acgaaacgtg aacagggact ctccgcggct tcccagaacc gcacagtatt caaaacccca
      301 ctgcttcgcg aactccggta ttacctgagc caaacggttt tcgactccaa ccagccgggt
      361 ggaggaccac gcatactgct cgagaaaagc ccagctcaac cggttcacct ctttggagta
      421 caagcgatcg agacgaaaga gaccctgctc gacttcgtga tgacgctcct gttcgagaat
      481 catgcgccgc tcttcgagaa attggcgggc ggcgaatccc atgatcagca gcgggatcat
      541 cgaagcgtac aagaaagctc ccagcagttt cgggcgaatc gaaccggcaa accagggctc
      601 gaggaaccag gccagacaac agatacccgc aagcaccagc gcccagccga gccactcgcg
      661 ccgactcgct cctcgccccg actgccgggc caaggttgct gttccgatcg cccaccacga
      721 cggcgaaagg atctgcctgg agatcaaagt cccgtccagc cacacggacc ccgacgcgtt
      781 gcgctgcagc aggttccagg cttcagaggc cgggacactc gttgccgcgc ttgccagcca
      841 ttcgctcgga ggctcgcctt ccgacgggat gatgttcagg cgtatcccac ggtaccggcg
      901 ctgcacctcc gcggccactt ctgaaagcgc cggcagaacc ggctgttcgg gggttcggca
      961 gaagagaatg agcatgccct tgcgatcggg ggcagacaga tagaaata
//
LOCUS       DAORHD010001768         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229834_length_1008_cov_3.076600, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001768 DAORHD010000000
VERSION     DAORHD010001768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229834_length_1008_cov_3.076600"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKO06_25115"
     CDS             complement(<1..236)
                     /locus_tag="PKO06_25115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73014.1"
                     /translation="MTRPAPGARTNEDCLLVGRQPVPHAVVCDGAGNAGGAAHRACRL
                     FKQLLAATSAQDLAAVETWLGFLTVLDAHVIGIG"
     gene            348..>1008
                     /locus_tag="PKO06_25120"
     CDS             348..>1008
                     /locus_tag="PKO06_25120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNV73015.1"
                     /translation="MEADLEFMEQMLSRLVQLEPGCHLHLEPFETEWLVRLSDGRQMT
                     ELAHMTHHQGIMLAQFLLKGADIFEQASKLPATGALKLSVGEREQWLQVSSTPVIWGA
                     KLVVGIMQTVLPRKNLDELGLEQSCLEMVQRAIAVRRGVVIVGGRSRSGRSTTERAML
                     SACVTDGMRGAIIEYADQGPLHGVESHIIGGPGAVAVAEVMRSLRSVPIEVIGTSIDS
                     GE"
BASE COUNT          196 a          283 c          342 g          187 t
ORIGIN      
        1 tcgccgatgc cgatcacatg ggcgtcgagc accgtgagaa accccagcca ggtctcgacc
       61 gcggcgaggt cttgcgccga agtcgcggcc agaagctgct tgaacagccg acaggcccga
      121 tgagccgcgc ccccggcgtt accggccccg tcgcagacga cggcatgcgg cacgggttgc
      181 cggccgacga gcagacagtc ttcgttcgtg cgggctcccg gagccggtct tgtcattccg
      241 tgaacgtcag gcatacaata gaatctacgc cgggatgatt cacggagcaa gtggcgttgt
      301 cgaagttcat tgccgaaagt cccggtcatt cgacaaggat acagccaatg gaagcagatc
      361 tcgagttcat ggaacagatg ctgtcgcggc tggtgcagct cgaacccggc tgccatctgc
      421 atcttgaacc gttcgaaacc gaatggctcg tgcggctcag cgatggccgg cagatgaccg
      481 aactggcaca tatgacgcat catcagggaa tcatgctggc gcagttcctg ctgaaagggg
      541 cggatatttt cgagcaggcg agcaaactgc cggcaaccgg ggccctgaaa ctttcggtcg
      601 gtgagcgtga gcagtggttg caggtttcca gcactccggt catctgggga gccaagctgg
      661 tcgtcggaat catgcagacc gtgctgcccc ggaagaacct ggacgaactt gggctcgaac
      721 agtcgtgtct cgagatggtg cagcgggcga tcgccgtaag gcgaggggtg gtgatcgtcg
      781 gcggacgttc gcggtcgggg cgctcgacca cggaacgggc gatgctgtct gcctgtgtta
      841 ctgacggcat gcggggtgcc atcatcgagt atgccgatca ggggccgctt cacggagtcg
      901 aatcgcacat catcggtggg ccgggtgccg ttgcggtcgc tgaggtgatg cggtcgctgc
      961 gaagtgtgcc gatcgaagtg attggcacct caatcgactc tggcgaag
//
LOCUS       DAORHD010001769         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229930_length_1008_cov_2.313746, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001769 DAORHD010000000
VERSION     DAORHD010001769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229930_length_1008_cov_2.313746"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PKO06_25125"
     CDS             complement(<1..280)
                     /locus_tag="PKO06_25125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV73016.1"
                     /translation="MNPGELLAPETEARLIKEVQAGRAESFSGLVESHWPRVRAVVRR
                     MVRDEGAVEDLCQEAFLRAYSKIEQFDVSRRFSPWLMKIAVNLVSEHFR"
     gene            complement(286..>1008)
                     /locus_tag="PKO06_25130"
     CDS             complement(286..>1008)
                     /locus_tag="PKO06_25130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73017.1"
                     /translation="AAPGSSGNASAPGAVGSPSQDISKPMSAGMSNVMTAPSPQGKSM
                     TGQEKSDYVYKTDAETDELQSAIAEFNRRFCPPQTPITEINRESIGILQKYEVLKEAP
                     QSLNDLVIRDGKVYRREKRDAAQQQQLGENPGSLFGSFGATAKSLDDQAPKSQGNKIV
                     DNPNLVEKRVTVRQKSGDYEEIKYTVPQVPGRVKSPPKKVPVRTRSDIVRRQILAPRY
                     KATVPIQPTDRPVTASSTAKTQ"
BASE COUNT          192 a          308 c          261 g          247 t
ORIGIN      
        1 gacgaaaatg ctctgagacc agattcacgg caattttcat caaccagggc gagaaacgtc
       61 tcgacacgtc gaactgttcg atcttgctgt aggcacgcag aaaagcctcc tggcagaggt
      121 cttccaccgc accttcgtca cgcaccatgc gcctcacgac ggctcgcacc cgtggccaat
      181 gactttccac cagtccagaa aaactctctg cccggccagc ctgcacttcc ttgatcaggc
      241 gcgcttcagt ttccggtgcc agcagttccc ctggattcat gcccgctact gcgttttcgc
      301 cgtactgctc gccgtgacgg gtctatccgt gggctggata ggaacggtcg ccttgtatct
      361 cggggccagg atctgcctgc gcacaatatc tgaacgcgtt cgaacgggga ctttcttcgg
      421 cggagacttc acccgccccg gcacttgtgg caccgtatac ttgatctctt catagtctcc
      481 agacttctgc cgcacggtca cacgcttttc gaccagattc ggattatcaa cgatcttgtt
      541 cccctgactc ttcggtgcct gatcatcaag cgatttggcc gtggctccaa aggaaccgaa
      601 cagagaccca ggattctccc ccagttgctg ctgttgcgca gcatcgcgtt tttcacgacg
      661 atagaccttg ccatcgcgaa tgacgaggtc attcagggac tggggagctt ctttcaacac
      721 ttcatatttc tgcagaatac cgatcgactc gcgattgatc tccgtgatcg gcgtctgcgg
      781 agggcagaaa cgccggttga attcggcgat tgcgctctgc agttcatcgg tctcagcgtc
      841 ggttttatag acatagtcag atttttcctg accggtcatg gatttcccct gcgggcttgg
      901 agcggtcatg acatttgaca tgcctgccga cattggcttg ctgatgtctt gcgacggact
      961 gccgacagct cccggagcgc tggcattgcc cgagcttcct ggcgcagc
//
LOCUS       DAORHD010001770         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_229972_length_1008_cov_2.103882, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001770 DAORHD010000000
VERSION     DAORHD010001770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229972_length_1008_cov_2.103882"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKO06_25135"
     CDS             <1..299
                     /locus_tag="PKO06_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73018.1"
                     /translation="LVGEIVKKVYDTLAALVPASVRANDSSEQQLARSMVHASDLRVV
                     LHLEQPAKHSKLFPRVYDPNHIYMKLKQLLKPIDPHPFVVETNRMGSLHWEVSS"
     gene            296..>1008
                     /locus_tag="PKO06_25140"
     CDS             296..>1008
                     /locus_tag="PKO06_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HNV73019.1"
                     /translation="MTTLKPWRETAIPHEDVLKGTFQQAEFAADISRVHDGTAVPEYA
                     DPVKFFARTFITEGMRLLLESVIKRISGKGGDPVIQLQTAFGGGKTHTLLAVYHLARG
                     LADPAQLDGVPDLLKSAGAKSLPQAKVAVLDGVKLSPSQPQKHGKHQTRTLWGELAWQ
                     LGGEAAYEQLKNADISGTSPGKDTLAKLLSEHAPCVILMDELVGYIRQFAEGTGLSGG
                     TYDSNLSFIQALTEAMKAVP"
BASE COUNT          222 a          306 c          273 g          207 t
ORIGIN      
        1 ctctggttgg agaaatcgtc aagaaagtct acgatacgtt ggccgcactc gtgccagcaa
       61 gtgtccgtgc taacgattcc tcagaacagc agttggcgcg cagtatggtc catgcttctg
      121 atcttcgggt tgtgttgcat ctcgaacagc ccgcgaagca ctccaagttg ttcccgcgcg
      181 tttatgatcc aaaccacatt tatatgaaac tgaaacagct tctgaagccg attgatcccc
      241 atcctttcgt cgtagaaaca aatcgcatgg gttcattaca ctgggaggta tcatcatgac
      301 aacgctgaaa ccctggcgtg aaacggctat tccgcatgaa gacgtgctga agggaacctt
      361 tcagcaggcg gagttcgcgg ctgacatctc gcgtgtgcat gatggcaccg ctgttcccga
      421 atacgctgac ccggtcaaat tctttgcccg caccttcatc accgagggca tgcgcctgct
      481 tctcgaatcg gtcatcaagc gcatctccgg caaaggcggc gacccggtca ttcaacttca
      541 gaccgcattc ggcggtggca aaacccacac cctgctcgcg gtctaccacc tggcccgtgg
      601 cctggctgac cccgcccagc tcgacggcgt tcccgatctg ctgaaatcag ccggtgccaa
      661 atctctgccg caagccaagg tcgccgttct cgacggggtg aagctctcgc ccagccagcc
      721 acagaagcat ggcaagcacc agacgcgcac gctctgggga gagctggcct ggcaactggg
      781 tggcgaagcc gcgtatgaac agctgaagaa cgccgacatc tcgggtacct caccgggaaa
      841 agacacgctg gcgaaactgt tgtccgagca cgctccatgc gtcatcctca tggatgagct
      901 ggtcggttac attcggcagt ttgccgaagg cacagggttg tcaggcggca cgtacgactc
      961 gaacctctcg ttcattcaag cgctgaccga ggcgatgaaa gctgtccc
//
LOCUS       DAORHD010001771         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_231445_length_1004_cov_2.874605, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001771 DAORHD010000000
VERSION     DAORHD010001771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231445_length_1004_cov_2.874605"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PKO06_25145"
     CDS             complement(<1..311)
                     /locus_tag="PKO06_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNV73020.1"
                     /translation="MGYSATLPSDIITRLGDIAEIINGSQRFLITAHLRPDGDATGSV
                     LGLARALRLAGKQADITLHDPIPERFRFLLGEELILRPDQLQSGYDTVLVLDSGDADR
                     TG"
     gene            complement(283..>1004)
                     /locus_tag="PKO06_25150"
     CDS             complement(283..>1004)
                     /locus_tag="PKO06_25150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNV73021.1"
                     /translation="MDDAFTKSHLHEEPAVKALKVMQDMLKAGTAYEGQGFDPQNDFK
                     SQRVAMIDQSIVSKVHMETGSGLSFPYGIAPLPGQATNAVILSGSNINIFNNGNPDKI
                     KGAWDFVKWFTSTEVGAEWARDTTYLPVRKSSIQHQILQDKLVQDPNLRAPFESLAYA
                     QFEPRIFCWFEIRDMMADHLERATLRPRSKLRDLADWAGLARLGLERFLPAPVADLPE
                     PGPHLLNLSKDIDGILRHSAK"
BASE COUNT          196 a          284 c          319 g          205 t
ORIGIN      
        1 cccgttcggt cggcgtctcc ggagtcaaga accaggaccg tgtcgtatcc gctctgcagc
       61 tgatctgggc gcagaatcaa ctcctcgccc agaagaaagc ggaatcgctc gggaatcggg
      121 tcatgcagcg tgatgtctgc ctgcttgccc gcgagccgca gcgcgcgcgc cagcccgaga
      181 accgaaccgg tcgcatcacc gtccggacgc agatgggcgg taatcagaaa acgctgcgag
      241 ccgttgatga tctcggcaat gtcgccgaga cgggtgatga tgtcacttgg cagagtggcg
      301 gagtatccca tcgatgtcct tgctcagatt gagaagatgc ggccccggtt caggcagatc
      361 agccacgggg gcgggaagaa aacgctcaag acccagcctg gccagaccgg cccagtctgc
      421 cagatcgcga agcttgctgc gggggcgcag cgtggcgcgt tcgaggtgat cggccatcat
      481 gtcgcgaatt tcgaaccagc agaagatgcg cggttcgaac tgggcgtagg cgagcgattc
      541 gaacggtgcg cgcaggttcg ggtcttgtac cagtttgtcc tgcaaaatct ggtgctgaat
      601 cgagcttttg cgcacgggca gatacgtcgt gtcgcgagcc cattccgcac cgacttcggt
      661 cgaggtgaac cacttgacga aatcccatgc tcccttgatt ttgtccgggt tgccgttgtt
      721 gaagatgttg atgttggaac cggagagaat gaccgcgttg gtcgcctgac cgggcagtgg
      781 ggcgattccg taagggaacg acagaccgct gcccgtttcc atgtgcacct tgctgacgat
      841 gctctgatcg atcatggcga cgcgctgcga cttgaaatcg ttctgcggat cgaacccctg
      901 cccttcatag gcggtacccg ccttgagcat gtcctgcatg actttcaggg ccttgacggc
      961 gggctcttca tgcagatgag acttggtgaa cgcgtcatcc atga
//
LOCUS       DAORHD010001772         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Ozemobacteraceae bacterium isolate
            AMR_MDS_1213 NODE_231789_length_1003_cov_3.400844, whole genome
            shotgun sequence.
ACCESSION   DAORHD010001772 DAORHD010000000
VERSION     DAORHD010001772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854418
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Ozemobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Candidatus Ozemobacteraceae bacterium
            Bacteria; Candidatus Ozemobacteria; Candidatus Ozemobacterales;
            Candidatus Ozemobacteraceae.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 19:38:07
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,031
            CDSs (total)                      :: 5,002
            Genes (coding)                    :: 4,978
            CDSs (with protein)               :: 4,978
            Genes (RNA)                       :: 29
            tRNAs                             :: 28
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 24
            CDSs (without protein)            :: 24
            Pseudo Genes (ambiguous residues) :: 0 of 24
            Pseudo Genes (frameshifted)       :: 2 of 24
            Pseudo Genes (incomplete)         :: 21 of 24
            Pseudo Genes (internal stop)      :: 2 of 24
            Pseudo Genes (multiple problems)  :: 1 of 24
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Candidatus Ozemobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231789_length_1003_cov_3.400844"
                     /isolate="AMR_MDS_1213"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2818468"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1003
                     /locus_tag="PKO06_25155"
     CDS             <1..>1003
                     /locus_tag="PKO06_25155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV73022.1"
                     /translation="DAPAAHGAVAEPAAHGAPTTDVAHEAESGHGEQGGHAAAKAAKV
                     TLVESGRLKALDEDRHREQKIDKVELPKAEPRYGIIGVIVLLALGFGLVVFLSAQSGA
                     FAKYSLAFKLYAGFAAIIVLMICMAVASNSFASQTQEALHQVAAATEMEMMAMDIKSL
                     LFEYLVYGLEDQKRADEVVKKAHEELDEFSTDAKDMQKGASDPKESEALDRVVAIAAG
                     YRKGFDSLVTAFDRVRSENAELRKAGQEVEHAVAEQLHHHEKELQEIQKRKGTDPRYI
                     SQQTELVTMLSEIELRVTQIESRESAFLLEKSLTDVHELEQLFGELNSQLQAAIDLLL
                     "
BASE COUNT          239 a          251 c          318 g          195 t
ORIGIN      
        1 gatgcacccg cggctcacgg agcggttgcc gagccggccg cccatggggc gccgacgacg
       61 gatgttgcgc atgaagctga atcaggccac ggagagcagg gaggccacgc tgcggcgaag
      121 gctgccaaag tcactctggt cgagagcggg cgattgaaag ccctggatga agatcgtcac
      181 cgcgagcaga aaattgacaa agtcgaactg ccgaaagccg agcctcggta tggcatcatc
      241 ggtgtgattg ttctgctggc gttgggtttc gggttggtgg tgtttctgtc ggcgcagagc
      301 ggggcgtttg ccaagtacag tctggccttc aagctgtatg cgggctttgc ggcgattatc
      361 gtgttgatga tctgcatggc ggtggcctca aattcgtttg cctcacagac acaagaggct
      421 ctgcatcagg ttgccgcagc caccgaaatg gaaatgatgg cgatggacat caagtcgctg
      481 ttatttgaat atctcgtcta cggactcgaa gaccagaagc gtgccgacga ggtagtcaag
      541 aaggcgcacg aagagctgga tgagttttcc accgacgcga aagacatgca aaaaggtgcg
      601 agcgatccta aagagtccga ggcgctcgat cgcgttgtcg cgatcgcggc cgggtatcga
      661 aaagggtttg acagcctcgt gaccgccttc gatcgggtgc gcagcgagaa tgccgaactg
      721 cgaaaagccg gtcaggaggt cgaacacgca gtcgctgaac aactgcatca tcacgagaaa
      781 gaacttcagg aaattcagaa acgcaaggga accgacccgc ggtatatctc tcaacagacg
      841 gaactggtga cgatgctgtc cgagatcgaa ttgcgggtca ctcagattga aagccgcgag
      901 tcggcctttc tgctcgagaa atctctgacc gatgtgcacg agcttgaaca gctgtttgga
      961 gaactgaaca gccaactgca ggctgcgatc gatctgctgc tga
//
